unc13a cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following unc13a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the unc13a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa42009 XM_018096799.1
Latest version!
Xenopus tropicalis unc-13 homolog A (unc13a), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OXa42010 XM_018096803.1
Latest version!
Xenopus tropicalis unc-13 homolog A (unc13a), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OXa42011 XM_018096807.1
Latest version!
Xenopus tropicalis unc-13 homolog A (unc13a), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OXa42012 XM_018096811.1
Latest version!
Xenopus tropicalis unc-13 homolog A (unc13a), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OXa42013 XM_018096818.1
Latest version!
Xenopus tropicalis unc-13 homolog A (unc13a), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OXa48429 XM_031904660.1
Latest version!
Xenopus tropicalis unc-13 homolog A (unc13a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa48430 XM_012954733.3
Latest version!
Xenopus tropicalis unc-13 homolog A (unc13a), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa48431 XM_031904665.1
Latest version!
Xenopus tropicalis unc-13 homolog A (unc13a), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa48432 XM_031904666.1
Latest version!
Xenopus tropicalis unc-13 homolog A (unc13a), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa48433 XM_031904667.1
Latest version!
Xenopus tropicalis unc-13 homolog A (unc13a), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa48434 XM_031904668.1
Latest version!
Xenopus tropicalis unc-13 homolog A (unc13a), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa48435 XM_012954752.3
Latest version!
Xenopus tropicalis unc-13 homolog A (unc13a), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa48436 XM_031904669.1
Latest version!
Xenopus tropicalis unc-13 homolog A (unc13a), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa48437 XM_012954756.3
Latest version!
Xenopus tropicalis unc-13 homolog A (unc13a), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa35340 XM_012954733.2
Latest version!
Xenopus tropicalis unc-13 homolog A (unc13a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OXa35345 XM_012954752.2
Latest version!
Xenopus tropicalis unc-13 homolog A (unc13a), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OXa35346 XM_012954756.2
Latest version!
Xenopus tropicalis unc-13 homolog A (unc13a), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa42009
    Clone ID Related Accession (Same CDS sequence) XM_018096799.1
    Accession Version XM_018096799.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5187bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein unc-13 homolog A isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGTCTTTGC TTTGCGTGGG AGTAAAAAAG GCCACGTTTG ATGGAGCACA AGAGAAGTTT 
    AATGCCTACG TAACCTTGAA GGTGCAAAAT GTGAAGAGCA CAACGATAGC GGTGCGGGGA
    AACCAGCCCT GCTGGGAACA GGATTTCATG TTTGAGATTA ATCGTTTAGA CCTTGGTCTA
    ACAGTGGAGG TCTGGAACAA AGGATTGATC TGGGACACCA TGGTGGGAAC AGTATGGATT
    CCTTTAAGGA CAATCCGACA ATCCAATGAG GAGGGTCCTG GGGAATGGCT GACACTTGAT
    TCACAGGTCA TAATGACAGA TAATGAGATT TGTGGTACCA AAGATCCAAC CTTCCACCGC
    ATCTTACTGG ATACCCGATT TGAGCTTCCT CTAGATATAC CAGAAGAAGA AGCTCGATAC
    TGGGCAAAGA AACTCGAGCA ACTTAATGCA ATGAGGGACC AGGATGATTA TGTTTTTCAA
    GAAGAAATTC CGGAGAATCC TCTTCCTGTC CATGGATCTC AATGTTGTAA CTGGAATTAT
    TTCGGCTGGG GGGAGCAGCA CAATGAGGAT CCCGATAGTG CAGTGGATGA TCGGGATAGT
    GATTACCGCA GTGAAACCAG CAACAGTATC CCGCCCCCCT ACTACTCCAC CTCACAGCCT
    AATGCCTCTG TGCACCAGTA CCCTGTACGA CAGCAACACA GTTCTCATGG ATCTTACACT
    GATTCAGTGC ACAGCTATGA GATGGACTTC TCTGACCATC GTCCAATCAG GCGTTACGAT
    AGCGCAGACT CTGCCACTAA CGGGCGCAGT GATGTGGAGT CTGTCTCCGG TTCGAGCCGC
    CTCACCAGCC GGCAGCACTC TCTGGAGAGC CGGCAGTCCT TAGACCTGTC CGATAGCCCC
    ACTGGAAGTA GTCGATATGC TTCATCAGGG GAGATTAGTC GTGGCTCCTC ACAGCTCAGT
    GATGACTTTG ACCCAGATGA TGACAGCTTG CAAGGTTCAG AAATAGAGGA AGAAAGGGAT
    AGAGACTCTT ATCACTCCTG TCATAGTTCT GTCAGCTGCC GCAAGGACTC GCCAAATTGG
    GAAGGAGAGG AGGAGCGTGA AGTTTATAGT GAAGATGAGG AAGAATATCC TGACAGTAGG
    GGGCAGCTCT CTGACCAGGA GCTGTATGAT GATGACCAGC TTGAGGAAGA AGAGAAAAAA
    GGAAGATTAG CATATGGGCA GGAGGATTCC ATCTATCAGT CATTGGAGGC TGAGGAGAAG
    GAACCCCTGG ATATTCCATT GGAGAAGGAC AGGACACCAG AAGCTGTGCC AGAGGAGGTA
    GTAGAGCCAA CAGTACCAGC AGTCCAGTCT CCCCAAGCTG CAGACAGTTA CAGAGAAGAA
    GAACCTGGAA TGTCCAGGGC CAAATCTAAT TGGTTAAGAG CATTCAACAA GGTGCGCCTG
    CAGCTGCAAG AGGCACGAGG TGAAGGGACT GGGGAGATGT CCAAATCCCT CTGGTTTAAG
    GATGGGCCAG CTGGTGGTCT TATAATTATA GACAGTATGC CAGACATACG AAAGAGGAAA
    CCAATCCCGT TGGTCAGTGA CCTGGCAATG TCACTGGTTC AGTCACGGAA GGCTGGGATT
    ACTTCTGCTC TTGCAACAAG CACCTTAAAC AATGAAGAGC TAAAAAACCA TGTTTACAAA
    AAGACCCTGC AAGCTTTAAT ATACCCAATA TCCAGCACCA CTCCCCACAA CTTTGAAGTT
    TGGACTGCCA CAACTCCTAC CTATTGTTAT GAATGCGAAG GGCTTCTGTG GGGAATCGCT
    CGGCAGGGCA TGCGTTGCAC AGAGTGTGGT GTCAAGTGCC ATGAGAAGTG CCAGGATCTG
    CTGAATGCAG ATTGTCTTCA AAGAGCTGCA GAGAAGAGCT CAAAACATGG TGCAGAAGAC
    CGGACTCAGA ATATTATTAT GGTCTTGAAG GATCGTATGA AGATCCGGGA GAGGAACAAG
    CCAGAAATAT TTGAGCTGAT CCAGGAAGTA TTTGGGGTAT TAAAGGCTAC ACACGCTCAG
    CAGATAAAGG CTGTGAAACA GAGTGTGCTG GATGGTACTT CCAAATGGTC AGCAAAGATC
    AGCATAACTG TTGTATGTGC ACAGGGTCTA CAGGCCAAAG ATAAGACAGG CTCCAGTGAC
    CCATATGTTA CTGTTCAAGT TGGGAAAACC AAGAAAAGAA CCAAAACAAT CTATGGAAAT
    CTTAATCCTG TCTGGGAAGA GAATTTTCAC TTTGAATGCC ACAACTCCTC AGATCGTATT
    AAAGTACGCG TGTGGGATGA AGATGATGAT ATCAAATCTC GTGTGAAGCA GAGATTTAAA
    AGAGAATCAG ATGACTTCCT TGGACAAACA ATTATTGAAG TCAGAACACT TAGCGGAGAG
    ATGGATGTGT GGTATAACTT GGATAAGAGA ACAGATAAAT CCGCTGTCTC TGGGGCAATC
    CGTCTTCATA TAAGTGTGGA AATAAAAGGA GAGGAAAAGG TGGCTCCCTA TCACGTGCAA
    TACACATGTC TACATGAGAA CTTATTCCAT TACCTGACGG ATGTGCAGAA TAATGGTGTG
    GTCAAGATTC CAGATGCAAA AGGAGACGAC GCCTGGAAAG TTTATTTTGA TGAGACAGCC
    CAGGAAATTG TGGATGAATT TGCAATGAGA TATGGAGTGG AGTCCATATA CCAGGCCATG
    ACGCATTTTG CCTGCCTTTC ATCTAAATAT ATGTGTCCAG GAGTACCAGC TGTAATGAGT
    ACTCTTCTTG CCAACATTAA TGCTTACTAT GCGCACACAA CTGCATCTAC TAATGTGTCT
    GCCTCCGACC GATTCTCTGC CTCAAACTTT GGGAAGGAGA GATTTGTAAA GCTGCTTGAC
    CAGTTGCACA ATTCCTTACG CATTGACCTG TCCATGTATC GGAACAACTT CCCTGCTAGC
    AGCCCTGAAA GACTCCAAGA TCTCAAGTCC ACTGTAGATT TACTAACTAG CATAACATTT
    TTCCGGATGA AGGTACAAGA GCTTCAGAGC CCACCACGGG CAAGCCAAGT TGTTAAGGAC
    TGTGTGAAGG CATGCCTTAA CTCAACCTAC GAATATATCT TTAACAACTG CCATGAACTT
    TATAGCCGAG AATATCAAAG TGACCCTGCT AAAAAAGGGG AGGTACCCCC AGAAGAACAG
    GGGCCAAGTA TAAAGAACTT AGATTTTTGG TCCAAACTAA TCACATTGAT TGTGTCTATC
    ATAGAGGAAG ATAAGAACTC ATATACACCT TGTCTAAACC AGTTTCCTCA GGAGCTAAAT
    GTTGGTAAGA TTAGTGCTGA GGTCATGTGG AGTCTCTTTG CTCAAGACAT GAGATATGCT
    ATGGAAGAAC ACGACAAACA CCGCCTGTGT AAGAGTGCAG ACTACATGAA CTTGCATTTT
    AAGGTCAAGT GGTTGTACAA TGAATATGTA ACTGAGCTAC CATCTTTTAA GAGCAAGGTT
    CCAGAATATC CCGCCTGGTT TGAGCCTTTT GTAATTCAGT GGCTGGATGA AAATGAAGAG
    GTCTCCCGGG ATTTTCTGCA TGGAGCACTT GAAAGAGATA AGAAGGATGG GTTCCAGCAG
    ACCTCTGAAC ATGCCCTGTT CTCCTGCTCT GTGGTAGATG TTTTCTCACA ACTTAACCAG
    AGCTTTGAGA TCATTAAGAA GTTGGAGTGT CCAGACCCAC AAATTGTGGG GCATTACATG
    CGTAGATTTG CCAAGACGAT CAGTAATGTT CTGCTGCAGT ATGCTGAAAT TATTTCCAAA
    GATTTTGCAT CTCATTGCTC CAAAGAGAAG AAAGAAGAAA AAGTGCCCTG TATTCTTATG
    AACAATATCC AGCAACTTCG AGTGCAACTA GAAAAAATGT TTGAAGCAAT GGGAGGAAAG
    GAGCTGGATG CAGAAACAAG TGACATTCTT AAAGAGCTGC AGGTGAAACT CAACAATGTC
    CTAGATGAGC TAAGCAGAGT GTTTACCACC AGTTTTCAGG CACAAATTGA GGAGTGTGTG
    AAGCAGATGG GTGACATTCT GAGCCAGGTA AAAGGGACCG GAAATGTACC AGCCAGTAAC
    TGCAGCAGTG TGGCTCAGGA TGCAGATAAT GTATTGCAGC CTATCCTGGA TCCTCTGGAC
    AGCAACCTGA CACTATTTGC CAAGATCTGT GAAAAGACAG TCCTGAAGCG GGTACTAAAA
    GAACTCTGGA AGCTTGTGAT GAATACAATG GAGAAAACGA TTGTCCTTCC ACCACTAACA
    GACCAAACAA TGATAAGCAG GGTGAAGCTG CCGTACCACG CAGATGGAAC CCACCTTATC
    ATTAATGCAG CCAAGGAACT GGGCCAGTTA TCCAAACTAA AGGATCACAT GGTGCGAGAG
    GAAGCCAAGA GCCTCACCCC TAAGCAGTGT GCAGTGGTGG AGCTTGCACT TGATACAATT
    AAGCAATATT TCCATGCTGG AGGTGTTGGT CTCAAGAAGA CATTCCTGGA GAAGAGTCCA
    GACCTACAAT CTCTACGCTA TGCACTTTCT CTTTACACAC AAGCAACCGA TACGCTAATC
    AAAACCTTCG TCCAGACACA GTCTGCTCAG GTTCATGGTG GTAAAGGTAT TAGGTTTACA
    CTTAATGAAG AGACTTATCC AGAAAAGGGA TCTGGGGTGG ATGATTCTGT TGGGGAAGTT
    TCTATCCATG TGGAGTTATT TACTCACCCA GGAACAGGCG AGCACAAGAT CAGCGTAAAG
    GTTGTAGCGG CCAATGACCT TAAATGGCAG ACGTCAGGGA TTTTCCGTCC CTTTATTGAG
    GTAAACATGA TTGGTCCGAA TCTGAGTGAC AAAAAAAGGA AATTTACCAC CAAGTCCAAG
    AACAACAGCT GGGCCCCAAA ATATAACGAG AGCTTCCAAT TTACCCTGGG TAATGAGACT
    GGTCCAGAGA GTTATGAGCT GCAAGTATGT GTGAAAGATT ACTGTTTTGC TCGGGAAGAC
    CGCACAGTGG GAATGGCTGT CATGCAACTG CGAGATCTGG CACAAAGGGG TAGCTGTGCC
    TGTTGGCTCC CTCTTGGCCG CCGCATTCAT ATGGATGAGA CAGGACTGAC CATCTTGCGC
    ATACTATCAC AAAGAAACAA TGATGAGGTG GCCAAAGAGT TTGTCAAACT CAAATCTGAC
    ACCCGCTCTC CAGAAGAGGG GAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017952288.1
    CDS188..5374
    Translation

    Target ORF information:

    RefSeq Version XM_018096799.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis unc-13 homolog A (unc13a), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018096799.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGTCTTTGC TTTGCGTGGG AGTAAAAAAG GCCACGTTTG ATGGAGCACA AGAGAAGTTT 
    AATGCCTACG TAACCTTGAA GGTGCAAAAT GTGAAGAGCA CAACGATAGC GGTGCGGGGA
    AACCAGCCCT GCTGGGAACA GGATTTCATG TTTGAGATTA ATCGTTTAGA CCTTGGTCTA
    ACAGTGGAGG TCTGGAACAA AGGATTGATC TGGGACACCA TGGTGGGAAC AGTATGGATT
    CCTTTAAGGA CAATCCGACA ATCCAATGAG GAGGGTCCTG GGGAATGGCT GACACTTGAT
    TCACAGGTCA TAATGACAGA TAATGAGATT TGTGGTACCA AAGATCCAAC CTTCCACCGC
    ATCTTACTGG ATACCCGATT TGAGCTTCCT CTAGATATAC CAGAAGAAGA AGCTCGATAC
    TGGGCAAAGA AACTCGAGCA ACTTAATGCA ATGAGGGACC AGGATGATTA TGTTTTTCAA
    GAAGAAATTC CGGAGAATCC TCTTCCTGTC CATGGATCTC AATGTTGTAA CTGGAATTAT
    TTCGGCTGGG GGGAGCAGCA CAATGAGGAT CCCGATAGTG CAGTGGATGA TCGGGATAGT
    GATTACCGCA GTGAAACCAG CAACAGTATC CCGCCCCCCT ACTACTCCAC CTCACAGCCT
    AATGCCTCTG TGCACCAGTA CCCTGTACGA CAGCAACACA GTTCTCATGG ATCTTACACT
    GATTCAGTGC ACAGCTATGA GATGGACTTC TCTGACCATC GTCCAATCAG GCGTTACGAT
    AGCGCAGACT CTGCCACTAA CGGGCGCAGT GATGTGGAGT CTGTCTCCGG TTCGAGCCGC
    CTCACCAGCC GGCAGCACTC TCTGGAGAGC CGGCAGTCCT TAGACCTGTC CGATAGCCCC
    ACTGGAAGTA GTCGATATGC TTCATCAGGG GAGATTAGTC GTGGCTCCTC ACAGCTCAGT
    GATGACTTTG ACCCAGATGA TGACAGCTTG CAAGGTTCAG AAATAGAGGA AGAAAGGGAT
    AGAGACTCTT ATCACTCCTG TCATAGTTCT GTCAGCTGCC GCAAGGACTC GCCAAATTGG
    GAAGGAGAGG AGGAGCGTGA AGTTTATAGT GAAGATGAGG AAGAATATCC TGACAGTAGG
    GGGCAGCTCT CTGACCAGGA GCTGTATGAT GATGACCAGC TTGAGGAAGA AGAGAAAAAA
    GGAAGATTAG CATATGGGCA GGAGGATTCC ATCTATCAGT CATTGGAGGC TGAGGAGAAG
    GAACCCCTGG ATATTCCATT GGAGAAGGAC AGGACACCAG AAGCTGTGCC AGAGGAGGTA
    GTAGAGCCAA CAGTACCAGC AGTCCAGTCT CCCCAAGCTG CAGACAGTTA CAGAGAAGAA
    GAACCTGGAA TGTCCAGGGC CAAATCTAAT TGGTTAAGAG CATTCAACAA GGTGCGCCTG
    CAGCTGCAAG AGGCACGAGG TGAAGGGACT GGGGAGATGT CCAAATCCCT CTGGTTTAAG
    GATGGGCCAG CTGGTGGTCT TATAATTATA GACAGTATGC CAGACATACG AAAGAGGAAA
    CCAATCCCGT TGGTCAGTGA CCTGGCAATG TCACTGGTTC AGTCACGGAA GGCTGGGATT
    ACTTCTGCTC TTGCAACAAG CACCTTAAAC AATGAAGAGC TAAAAAACCA TGTTTACAAA
    AAGACCCTGC AAGCTTTAAT ATACCCAATA TCCAGCACCA CTCCCCACAA CTTTGAAGTT
    TGGACTGCCA CAACTCCTAC CTATTGTTAT GAATGCGAAG GGCTTCTGTG GGGAATCGCT
    CGGCAGGGCA TGCGTTGCAC AGAGTGTGGT GTCAAGTGCC ATGAGAAGTG CCAGGATCTG
    CTGAATGCAG ATTGTCTTCA AAGAGCTGCA GAGAAGAGCT CAAAACATGG TGCAGAAGAC
    CGGACTCAGA ATATTATTAT GGTCTTGAAG GATCGTATGA AGATCCGGGA GAGGAACAAG
    CCAGAAATAT TTGAGCTGAT CCAGGAAGTA TTTGGGGTAT TAAAGGCTAC ACACGCTCAG
    CAGATAAAGG CTGTGAAACA GAGTGTGCTG GATGGTACTT CCAAATGGTC AGCAAAGATC
    AGCATAACTG TTGTATGTGC ACAGGGTCTA CAGGCCAAAG ATAAGACAGG CTCCAGTGAC
    CCATATGTTA CTGTTCAAGT TGGGAAAACC AAGAAAAGAA CCAAAACAAT CTATGGAAAT
    CTTAATCCTG TCTGGGAAGA GAATTTTCAC TTTGAATGCC ACAACTCCTC AGATCGTATT
    AAAGTACGCG TGTGGGATGA AGATGATGAT ATCAAATCTC GTGTGAAGCA GAGATTTAAA
    AGAGAATCAG ATGACTTCCT TGGACAAACA ATTATTGAAG TCAGAACACT TAGCGGAGAG
    ATGGATGTGT GGTATAACTT GGATAAGAGA ACAGATAAAT CCGCTGTCTC TGGGGCAATC
    CGTCTTCATA TAAGTGTGGA AATAAAAGGA GAGGAAAAGG TGGCTCCCTA TCACGTGCAA
    TACACATGTC TACATGAGAA CTTATTCCAT TACCTGACGG ATGTGCAGAA TAATGGTGTG
    GTCAAGATTC CAGATGCAAA AGGAGACGAC GCCTGGAAAG TTTATTTTGA TGAGACAGCC
    CAGGAAATTG TGGATGAATT TGCAATGAGA TATGGAGTGG AGTCCATATA CCAGGCCATG
    ACGCATTTTG CCTGCCTTTC ATCTAAATAT ATGTGTCCAG GAGTACCAGC TGTAATGAGT
    ACTCTTCTTG CCAACATTAA TGCTTACTAT GCGCACACAA CTGCATCTAC TAATGTGTCT
    GCCTCCGACC GATTCTCTGC CTCAAACTTT GGGAAGGAGA GATTTGTAAA GCTGCTTGAC
    CAGTTGCACA ATTCCTTACG CATTGACCTG TCCATGTATC GGAACAACTT CCCTGCTAGC
    AGCCCTGAAA GACTCCAAGA TCTCAAGTCC ACTGTAGATT TACTAACTAG CATAACATTT
    TTCCGGATGA AGGTACAAGA GCTTCAGAGC CCACCACGGG CAAGCCAAGT TGTTAAGGAC
    TGTGTGAAGG CATGCCTTAA CTCAACCTAC GAATATATCT TTAACAACTG CCATGAACTT
    TATAGCCGAG AATATCAAAG TGACCCTGCT AAAAAAGGGG AGGTACCCCC AGAAGAACAG
    GGGCCAAGTA TAAAGAACTT AGATTTTTGG TCCAAACTAA TCACATTGAT TGTGTCTATC
    ATAGAGGAAG ATAAGAACTC ATATACACCT TGTCTAAACC AGTTTCCTCA GGAGCTAAAT
    GTTGGTAAGA TTAGTGCTGA GGTCATGTGG AGTCTCTTTG CTCAAGACAT GAGATATGCT
    ATGGAAGAAC ACGACAAACA CCGCCTGTGT AAGAGTGCAG ACTACATGAA CTTGCATTTT
    AAGGTCAAGT GGTTGTACAA TGAATATGTA ACTGAGCTAC CATCTTTTAA GAGCAAGGTT
    CCAGAATATC CCGCCTGGTT TGAGCCTTTT GTAATTCAGT GGCTGGATGA AAATGAAGAG
    GTCTCCCGGG ATTTTCTGCA TGGAGCACTT GAAAGAGATA AGAAGGATGG GTTCCAGCAG
    ACCTCTGAAC ATGCCCTGTT CTCCTGCTCT GTGGTAGATG TTTTCTCACA ACTTAACCAG
    AGCTTTGAGA TCATTAAGAA GTTGGAGTGT CCAGACCCAC AAATTGTGGG GCATTACATG
    CGTAGATTTG CCAAGACGAT CAGTAATGTT CTGCTGCAGT ATGCTGAAAT TATTTCCAAA
    GATTTTGCAT CTCATTGCTC CAAAGAGAAG AAAGAAGAAA AAGTGCCCTG TATTCTTATG
    AACAATATCC AGCAACTTCG AGTGCAACTA GAAAAAATGT TTGAAGCAAT GGGAGGAAAG
    GAGCTGGATG CAGAAACAAG TGACATTCTT AAAGAGCTGC AGGTGAAACT CAACAATGTC
    CTAGATGAGC TAAGCAGAGT GTTTACCACC AGTTTTCAGG CACAAATTGA GGAGTGTGTG
    AAGCAGATGG GTGACATTCT GAGCCAGGTA AAAGGGACCG GAAATGTACC AGCCAGTAAC
    TGCAGCAGTG TGGCTCAGGA TGCAGATAAT GTATTGCAGC CTATCCTGGA TCCTCTGGAC
    AGCAACCTGA CACTATTTGC CAAGATCTGT GAAAAGACAG TCCTGAAGCG GGTACTAAAA
    GAACTCTGGA AGCTTGTGAT GAATACAATG GAGAAAACGA TTGTCCTTCC ACCACTAACA
    GACCAAACAA TGATAAGCAG GGTGAAGCTG CCGTACCACG CAGATGGAAC CCACCTTATC
    ATTAATGCAG CCAAGGAACT GGGCCAGTTA TCCAAACTAA AGGATCACAT GGTGCGAGAG
    GAAGCCAAGA GCCTCACCCC TAAGCAGTGT GCAGTGGTGG AGCTTGCACT TGATACAATT
    AAGCAATATT TCCATGCTGG AGGTGTTGGT CTCAAGAAGA CATTCCTGGA GAAGAGTCCA
    GACCTACAAT CTCTACGCTA TGCACTTTCT CTTTACACAC AAGCAACCGA TACGCTAATC
    AAAACCTTCG TCCAGACACA GTCTGCTCAG GTTCATGGTG GTAAAGGTAT TAGGTTTACA
    CTTAATGAAG AGACTTATCC AGAAAAGGGA TCTGGGGTGG ATGATTCTGT TGGGGAAGTT
    TCTATCCATG TGGAGTTATT TACTCACCCA GGAACAGGCG AGCACAAGAT CAGCGTAAAG
    GTTGTAGCGG CCAATGACCT TAAATGGCAG ACGTCAGGGA TTTTCCGTCC CTTTATTGAG
    GTAAACATGA TTGGTCCGAA TCTGAGTGAC AAAAAAAGGA AATTTACCAC CAAGTCCAAG
    AACAACAGCT GGGCCCCAAA ATATAACGAG AGCTTCCAAT TTACCCTGGG TAATGAGACT
    GGTCCAGAGA GTTATGAGCT GCAAGTATGT GTGAAAGATT ACTGTTTTGC TCGGGAAGAC
    CGCACAGTGG GAATGGCTGT CATGCAACTG CGAGATCTGG CACAAAGGGG TAGCTGTGCC
    TGTTGGCTCC CTCTTGGCCG CCGCATTCAT ATGGATGAGA CAGGACTGAC CATCTTGCGC
    ATACTATCAC AAAGAAACAA TGATGAGGTG GCCAAAGAGT TTGTCAAACT CAAATCTGAC
    ACCCGCTCTC CAGAAGAGGG GAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa42010
    Clone ID Related Accession (Same CDS sequence) XM_018096803.1
    Accession Version XM_018096803.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5169bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein unc-13 homolog A isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGTCTTTGC TTTGCGTGGG AGTAAAAAAG GCCACGTTTG ATGGAGCACA AGAGAAGTTT 
    AATGCCTACG TAACCTTGAA GGTGCAAAAT GTGAAGAGCA CAACGATAGC GGTGCGGGGA
    AACCAGCCCT GCTGGGAACA GGATTTCATG TTTGAGATTA ATCGTTTAGA CCTTGGTCTA
    ACAGTGGAGG TCTGGAACAA AGGATTGATC TGGGACACCA TGGTGGGAAC AGTATGGATT
    CCTTTAAGGA CAATCCGACA ATCCAATGAG GAGGGTCCTG GGGAATGGCT GACACTTGAT
    TCACAGGTCA TAATGACAGA TAATGAGATT TGTGGTACCA AAGATCCAAC CTTCCACCGC
    ATCTTACTGG ATACCCGATT TGAGCTTCCT CTAGATATAC CAGAAGAAGA AGCTCGATAC
    TGGGCAAAGA AACTCGAGCA ACTTAATGCA ATGAGGGACC AGGATGATTA TGTTTTTCAA
    GAAGAAATTC CGGAGAATCC TCTTCCTGTC CATGGATCTC AATGTTGTAA CTGGAATTAT
    TTCGGCTGGG GGGAGCAGCA CAATGAGGAT CCCGATAGTG CAGTGGATGA TCGGGATAGT
    GATTACCGCA GTGAAACCAG CAACAGTATC CCGCCCCCCT ACTACTCCAC CTCACAGCCT
    AATGCCTCTG TGCACCAGTA CCCTGTACGA CAGCAACACA GTTCTCATGG ATCTTACACT
    GATTCAGTGC ACAGCTATGA GATGGACTTC TCTGACCATC GTCCAATCAG GCGTTACGAT
    AGCGCAGACT CTGCCACTAA CGGGCGCAGT GATGTGGAGT CTGTCTCCGG TTCGAGCCGC
    CTCACCAGCC GGCAGCACTC TCTGGAGAGC CGGCAGTCCT TAGACCTGTC CGATAGCCCC
    ACTGGAAGTA GTCGATATGC TTCATCAGGG GAGATTAGTC GTGGCTCCTC ACAGCTCAGT
    GATGACTTTG ACCCAGATGA TGACAGCTTG CAAGGTTCAG AAATAGAGGA AGAAAGGGAT
    AGAGACTCTT ATCACTCCTG TCATAGTTCT GTCAGCTGCC GCAAGGACTC GCCAAATTGG
    GAAGGAGAGG AGGAGCGTGA AGTTTATAGT GAAGATGAGG AAGAATATCC TGACAGTAGG
    GGGCAGCTCT CTGACCAGGA GCTGTATGAT GATGACCAGC TTGAGGAAGA AGAGAAAAAA
    GGAAGATTAG CATATGGGCA GGAGGATTCC ATCTATCAGT CATTGGAGGC TGAGGAGAAG
    GAACCCCTGG ATATTCCATT GGAGAAGGAC AGGACACCAG AAGCTGTGCC AGAGGAGGTA
    GTAGAGCCAA CAGTACCAGC AGTCCAGTCT CCCCAAGCTG CAGACAGTTA CAGAGAAGAA
    GAACCTGGAA TGTCCAGGGC CAAATCTAAT TGGTTAAGAG CATTCAACAA GGTGCGCCTG
    CAGCTGCAAG AGGCACGAGG TGAAGGGACT GGGGAGATGT CCAAATCCCT CTGGTTTAAG
    GATGGGCCAG CTGGTGGTCT TATAATTATA GACAGTATGC CAGACATACG AAAGAGGAAA
    CCAATCCCGT TGGTCAGTGA CCTGGCAATG TCACTGGTTC AGTCACGGAA GGCTGGGATT
    ACTTCTGCTC TTGCAACAAG CACCTTAAAC AATGAAGAGC TAAAAAACCA TGTTTACAAA
    AAGACCCTGC AAGCTTTAAT ATACCCAATA TCCAGCACCA CTCCCCACAA CTTTGAAGTT
    TGGACTGCCA CAACTCCTAC CTATTGTTAT GAATGCGAAG GGCTTCTGTG GGGAATCGCT
    CGGCAGGGCA TGCGTTGCAC AGAGTGTGGT GTCAAGTGCC ATGAGAAGTG CCAGGATCTG
    CTGAATGCAG ATTGTCTTCA AAGAGCTGCA GAGAAGAGCT CAAAACATGG TGCAGAAGAC
    CGGACTCAGA ATATTATTAT GGTCTTGAAG GATCGTATGA AGATCCGGGA GAGGAACAAG
    CCAGAAATAT TTGAGCTGAT CCAGGAAGTA TTTGGGGTAT TAAAGGCTAC ACACGCTCAG
    CAGATAAAGG CTGTGAAACA GAGTGTGCTG GATGGTACTT CCAAATGGTC AGCAAAGATC
    AGCATAACTG TTGTATGTGC ACAGGGTCTA CAGGCCAAAG ATAAGACAGG CTCCAGTGAC
    CCATATGTTA CTGTTCAAGT TGGGAAAACC AAGAAAAGAA CCAAAACAAT CTATGGAAAT
    CTTAATCCTG TCTGGGAAGA GAATTTTCAC TTTGAATGCC ACAACTCCTC AGATCGTATT
    AAAGTACGCG TGTGGGATGA AGATGATGAT ATCAAATCTC GTGTGAAGCA GAGATTTAAA
    AGAGAATCAG ATGACTTCCT TGGACAAACA ATTATTGAAG TCAGAACACT TAGCGGAGAG
    ATGGATGTGT GGTATAACTT GGATAAGAGA ACAGATAAAT CCGCTGTCTC TGGGGCAATC
    CGTCTTCATA TAAGTGTGGA AATAAAAGGA GAGGAAAAGG TGGCTCCCTA TCACGTGCAA
    TACACATGTC TACATGAGAA CTTATTCCAT TACCTGACGG ATGTGCAGAA TAATGGTGTG
    GTCAAGATTC CAGATGCAAA AGGAGACGAC GCCTGGAAAG TTTATTTTGA TGAGACAGCC
    CAGGAAATTG TGGATGAATT TGCAATGAGA TATGGAGTGG AGTCCATATA CCAGGCCATG
    ACGCATTTTG CCTGCCTTTC ATCTAAATAT ATGTGTCCAG GAGTACCAGC TGTAATGAGT
    ACTCTTCTTG CCAACATTAA TGCTTACTAT GCGCACACAA CTGCATCTAC TAATGTGTCT
    GCCTCCGACC GATTCTCTGC CTCAAACTTT GGGAAGGAGA GATTTGTAAA GCTGCTTGAC
    CAGTTGCACA ATTCCTTACG CATTGACCTG TCCATGTATC GGAACAACTT CCCTGCTAGC
    AGCCCTGAAA GACTCCAAGA TCTCAAGTCC ACTGTAGATT TACTAACTAG CATAACATTT
    TTCCGGATGA AGGTACAAGA GCTTCAGAGC CCACCACGGG CAAGCCAAGT TGTTAAGGAC
    TGTGTGAAGG CATGCCTTAA CTCAACCTAC GAATATATCT TTAACAACTG CCATGAACTT
    TATAGCCGAG AATATCAAAG TGACCCTGCT AAAAAAGGGG AGGTACCCCC AGAAGAACAG
    GGGCCAAGTA TAAAGAACTT AGATTTTTGG TCCAAACTAA TCACATTGAT TGTGTCTATC
    ATAGAGGAAG ATAAGAACTC ATATACACCT TGTCTAAACC AGTTTCCTCA GGAGCTAAAT
    GTTGGTAAGA TTAGTGCTGA GGTCATGTGG AGTCTCTTTG CTCAAGACAT GAGATATGCT
    ATGGAAGAAC ACGACAAACA CCGCCTGTGT AAGAGTGCAG ACTACATGAA CTTGCATTTT
    AAGGTCAAGT GGTTGTACAA TGAATATGTA ACTGAGCTAC CATCTTTTAA GAGCAAGGTT
    CCAGAATATC CCGCCTGGTT TGAGCCTTTT GTAATTCAGT GGCTGGATGA AAATGAAGAG
    GTCTCCCGGG ATTTTCTGCA TGGAGCACTT GAAAGAGATA AGAAGGATGG GTTCCAGCAG
    ACCTCTGAAC ATGCCCTGTT CTCCTGCTCT GTGGTAGATG TTTTCTCACA ACTTAACCAG
    AGCTTTGAGA TCATTAAGAA GTTGGAGTGT CCAGACCCAC AAATTGTGGG GCATTACATG
    CGTAGATTTG CCAAGACGAT CAGTAATGTT CTGCTGCAGT ATGCTGAAAT TATTTCCAAA
    GATTTTGCAT CTCATTGCTC CAAAGAGAAG AAAGAAGAAA AAGTGCCCTG TATTCTTATG
    AACAATATCC AGCAACTTCG AGTGCAACTA GAAAAAATGT TTGAAGCAAT GGGAGGAAAG
    GAGCTGGATG CAGAAACAAG TGACATTCTT AAAGAGCTGC AGGTGAAACT CAACAATGTC
    CTAGATGAGC TAAGCAGAGT GTTTACCACC AGTTTTCAGG CACAAATTGA GGAGTGTGTG
    AAGCAGATGG GTGACATTCT GAGCCAGGTA AAAGGGACCG GAAATGTACC AGCCAGTAAC
    TGCAGCAGTG TGGCTCAGGA TGCAGATAAT GTATTGCAGC CTATCCTGGA TCCTCTGGAC
    AGCAACCTGA CACTATTTGC CAAGATCTGT GAAAAGACAG TCCTGAAGCG GGTACTAAAA
    GAACTCTGGA AGCTTGTGAT GAATACAATG GAGAAAACGA TTGTCCTTCC ACCACTAACA
    GACCAAACAA TGATAGGGAC GCTGCTGAGA AAACATGGCA AGGGAATAGA GAAGAGCAGG
    GTGAAGCTGC CGTACCACGC AGATGGAACC CACCTTATCA TTAATGCAGC CAAGGAACTG
    GGCCAGTTAT CCAAACTAAA GGATCACATG GTGCGAGAGG AAGCCAAGAG CCTCACCCCT
    AAGCAGTGTG CAGTGGTGGA GCTTGCACTT GATACAATTA AGCAATATTT CCATGCTGGA
    GGTGTTGGTC TCAAGAAGAC ATTCCTGGAG AAGAGTCCAG ACCTACAATC TCTACGCTAT
    GCACTTTCTC TTTACACACA AGCAACCGAT ACGCTAATCA AAACCTTCGT CCAGACACAG
    TCTGCTCAGG GATCTGGGGT GGATGATTCT GTTGGGGAAG TTTCTATCCA TGTGGAGTTA
    TTTACTCACC CAGGAACAGG CGAGCACAAG ATCAGCGTAA AGGTTGTAGC GGCCAATGAC
    CTTAAATGGC AGACGTCAGG GATTTTCCGT CCCTTTATTG AGGTAAACAT GATTGGTCCG
    AATCTGAGTG ACAAAAAAAG GAAATTTACC ACCAAGTCCA AGAACAACAG CTGGGCCCCA
    AAATATAACG AGAGCTTCCA ATTTACCCTG GGTAATGAGA CTGGTCCAGA GAGTTATGAG
    CTGCAAGTAT GTGTGAAAGA TTACTGTTTT GCTCGGGAAG ACCGCACAGT GGGAATGGCT
    GTCATGCAAC TGCGAGATCT GGCACAAAGG GGTAGCTGTG CCTGTTGGCT CCCTCTTGGC
    CGCCGCATTC ATATGGATGA GACAGGACTG ACCATCTTGC GCATACTATC ACAAAGAAAC
    AATGATGAGG TGGCCAAAGA GTTTGTCAAA CTCAAATCTG ACACCCGCTC TCCAGAAGAG
    GGGAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017952292.1
    CDS188..5356
    Translation

    Target ORF information:

    RefSeq Version XM_018096803.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis unc-13 homolog A (unc13a), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018096803.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGTCTTTGC TTTGCGTGGG AGTAAAAAAG GCCACGTTTG ATGGAGCACA AGAGAAGTTT 
    AATGCCTACG TAACCTTGAA GGTGCAAAAT GTGAAGAGCA CAACGATAGC GGTGCGGGGA
    AACCAGCCCT GCTGGGAACA GGATTTCATG TTTGAGATTA ATCGTTTAGA CCTTGGTCTA
    ACAGTGGAGG TCTGGAACAA AGGATTGATC TGGGACACCA TGGTGGGAAC AGTATGGATT
    CCTTTAAGGA CAATCCGACA ATCCAATGAG GAGGGTCCTG GGGAATGGCT GACACTTGAT
    TCACAGGTCA TAATGACAGA TAATGAGATT TGTGGTACCA AAGATCCAAC CTTCCACCGC
    ATCTTACTGG ATACCCGATT TGAGCTTCCT CTAGATATAC CAGAAGAAGA AGCTCGATAC
    TGGGCAAAGA AACTCGAGCA ACTTAATGCA ATGAGGGACC AGGATGATTA TGTTTTTCAA
    GAAGAAATTC CGGAGAATCC TCTTCCTGTC CATGGATCTC AATGTTGTAA CTGGAATTAT
    TTCGGCTGGG GGGAGCAGCA CAATGAGGAT CCCGATAGTG CAGTGGATGA TCGGGATAGT
    GATTACCGCA GTGAAACCAG CAACAGTATC CCGCCCCCCT ACTACTCCAC CTCACAGCCT
    AATGCCTCTG TGCACCAGTA CCCTGTACGA CAGCAACACA GTTCTCATGG ATCTTACACT
    GATTCAGTGC ACAGCTATGA GATGGACTTC TCTGACCATC GTCCAATCAG GCGTTACGAT
    AGCGCAGACT CTGCCACTAA CGGGCGCAGT GATGTGGAGT CTGTCTCCGG TTCGAGCCGC
    CTCACCAGCC GGCAGCACTC TCTGGAGAGC CGGCAGTCCT TAGACCTGTC CGATAGCCCC
    ACTGGAAGTA GTCGATATGC TTCATCAGGG GAGATTAGTC GTGGCTCCTC ACAGCTCAGT
    GATGACTTTG ACCCAGATGA TGACAGCTTG CAAGGTTCAG AAATAGAGGA AGAAAGGGAT
    AGAGACTCTT ATCACTCCTG TCATAGTTCT GTCAGCTGCC GCAAGGACTC GCCAAATTGG
    GAAGGAGAGG AGGAGCGTGA AGTTTATAGT GAAGATGAGG AAGAATATCC TGACAGTAGG
    GGGCAGCTCT CTGACCAGGA GCTGTATGAT GATGACCAGC TTGAGGAAGA AGAGAAAAAA
    GGAAGATTAG CATATGGGCA GGAGGATTCC ATCTATCAGT CATTGGAGGC TGAGGAGAAG
    GAACCCCTGG ATATTCCATT GGAGAAGGAC AGGACACCAG AAGCTGTGCC AGAGGAGGTA
    GTAGAGCCAA CAGTACCAGC AGTCCAGTCT CCCCAAGCTG CAGACAGTTA CAGAGAAGAA
    GAACCTGGAA TGTCCAGGGC CAAATCTAAT TGGTTAAGAG CATTCAACAA GGTGCGCCTG
    CAGCTGCAAG AGGCACGAGG TGAAGGGACT GGGGAGATGT CCAAATCCCT CTGGTTTAAG
    GATGGGCCAG CTGGTGGTCT TATAATTATA GACAGTATGC CAGACATACG AAAGAGGAAA
    CCAATCCCGT TGGTCAGTGA CCTGGCAATG TCACTGGTTC AGTCACGGAA GGCTGGGATT
    ACTTCTGCTC TTGCAACAAG CACCTTAAAC AATGAAGAGC TAAAAAACCA TGTTTACAAA
    AAGACCCTGC AAGCTTTAAT ATACCCAATA TCCAGCACCA CTCCCCACAA CTTTGAAGTT
    TGGACTGCCA CAACTCCTAC CTATTGTTAT GAATGCGAAG GGCTTCTGTG GGGAATCGCT
    CGGCAGGGCA TGCGTTGCAC AGAGTGTGGT GTCAAGTGCC ATGAGAAGTG CCAGGATCTG
    CTGAATGCAG ATTGTCTTCA AAGAGCTGCA GAGAAGAGCT CAAAACATGG TGCAGAAGAC
    CGGACTCAGA ATATTATTAT GGTCTTGAAG GATCGTATGA AGATCCGGGA GAGGAACAAG
    CCAGAAATAT TTGAGCTGAT CCAGGAAGTA TTTGGGGTAT TAAAGGCTAC ACACGCTCAG
    CAGATAAAGG CTGTGAAACA GAGTGTGCTG GATGGTACTT CCAAATGGTC AGCAAAGATC
    AGCATAACTG TTGTATGTGC ACAGGGTCTA CAGGCCAAAG ATAAGACAGG CTCCAGTGAC
    CCATATGTTA CTGTTCAAGT TGGGAAAACC AAGAAAAGAA CCAAAACAAT CTATGGAAAT
    CTTAATCCTG TCTGGGAAGA GAATTTTCAC TTTGAATGCC ACAACTCCTC AGATCGTATT
    AAAGTACGCG TGTGGGATGA AGATGATGAT ATCAAATCTC GTGTGAAGCA GAGATTTAAA
    AGAGAATCAG ATGACTTCCT TGGACAAACA ATTATTGAAG TCAGAACACT TAGCGGAGAG
    ATGGATGTGT GGTATAACTT GGATAAGAGA ACAGATAAAT CCGCTGTCTC TGGGGCAATC
    CGTCTTCATA TAAGTGTGGA AATAAAAGGA GAGGAAAAGG TGGCTCCCTA TCACGTGCAA
    TACACATGTC TACATGAGAA CTTATTCCAT TACCTGACGG ATGTGCAGAA TAATGGTGTG
    GTCAAGATTC CAGATGCAAA AGGAGACGAC GCCTGGAAAG TTTATTTTGA TGAGACAGCC
    CAGGAAATTG TGGATGAATT TGCAATGAGA TATGGAGTGG AGTCCATATA CCAGGCCATG
    ACGCATTTTG CCTGCCTTTC ATCTAAATAT ATGTGTCCAG GAGTACCAGC TGTAATGAGT
    ACTCTTCTTG CCAACATTAA TGCTTACTAT GCGCACACAA CTGCATCTAC TAATGTGTCT
    GCCTCCGACC GATTCTCTGC CTCAAACTTT GGGAAGGAGA GATTTGTAAA GCTGCTTGAC
    CAGTTGCACA ATTCCTTACG CATTGACCTG TCCATGTATC GGAACAACTT CCCTGCTAGC
    AGCCCTGAAA GACTCCAAGA TCTCAAGTCC ACTGTAGATT TACTAACTAG CATAACATTT
    TTCCGGATGA AGGTACAAGA GCTTCAGAGC CCACCACGGG CAAGCCAAGT TGTTAAGGAC
    TGTGTGAAGG CATGCCTTAA CTCAACCTAC GAATATATCT TTAACAACTG CCATGAACTT
    TATAGCCGAG AATATCAAAG TGACCCTGCT AAAAAAGGGG AGGTACCCCC AGAAGAACAG
    GGGCCAAGTA TAAAGAACTT AGATTTTTGG TCCAAACTAA TCACATTGAT TGTGTCTATC
    ATAGAGGAAG ATAAGAACTC ATATACACCT TGTCTAAACC AGTTTCCTCA GGAGCTAAAT
    GTTGGTAAGA TTAGTGCTGA GGTCATGTGG AGTCTCTTTG CTCAAGACAT GAGATATGCT
    ATGGAAGAAC ACGACAAACA CCGCCTGTGT AAGAGTGCAG ACTACATGAA CTTGCATTTT
    AAGGTCAAGT GGTTGTACAA TGAATATGTA ACTGAGCTAC CATCTTTTAA GAGCAAGGTT
    CCAGAATATC CCGCCTGGTT TGAGCCTTTT GTAATTCAGT GGCTGGATGA AAATGAAGAG
    GTCTCCCGGG ATTTTCTGCA TGGAGCACTT GAAAGAGATA AGAAGGATGG GTTCCAGCAG
    ACCTCTGAAC ATGCCCTGTT CTCCTGCTCT GTGGTAGATG TTTTCTCACA ACTTAACCAG
    AGCTTTGAGA TCATTAAGAA GTTGGAGTGT CCAGACCCAC AAATTGTGGG GCATTACATG
    CGTAGATTTG CCAAGACGAT CAGTAATGTT CTGCTGCAGT ATGCTGAAAT TATTTCCAAA
    GATTTTGCAT CTCATTGCTC CAAAGAGAAG AAAGAAGAAA AAGTGCCCTG TATTCTTATG
    AACAATATCC AGCAACTTCG AGTGCAACTA GAAAAAATGT TTGAAGCAAT GGGAGGAAAG
    GAGCTGGATG CAGAAACAAG TGACATTCTT AAAGAGCTGC AGGTGAAACT CAACAATGTC
    CTAGATGAGC TAAGCAGAGT GTTTACCACC AGTTTTCAGG CACAAATTGA GGAGTGTGTG
    AAGCAGATGG GTGACATTCT GAGCCAGGTA AAAGGGACCG GAAATGTACC AGCCAGTAAC
    TGCAGCAGTG TGGCTCAGGA TGCAGATAAT GTATTGCAGC CTATCCTGGA TCCTCTGGAC
    AGCAACCTGA CACTATTTGC CAAGATCTGT GAAAAGACAG TCCTGAAGCG GGTACTAAAA
    GAACTCTGGA AGCTTGTGAT GAATACAATG GAGAAAACGA TTGTCCTTCC ACCACTAACA
    GACCAAACAA TGATAGGGAC GCTGCTGAGA AAACATGGCA AGGGAATAGA GAAGAGCAGG
    GTGAAGCTGC CGTACCACGC AGATGGAACC CACCTTATCA TTAATGCAGC CAAGGAACTG
    GGCCAGTTAT CCAAACTAAA GGATCACATG GTGCGAGAGG AAGCCAAGAG CCTCACCCCT
    AAGCAGTGTG CAGTGGTGGA GCTTGCACTT GATACAATTA AGCAATATTT CCATGCTGGA
    GGTGTTGGTC TCAAGAAGAC ATTCCTGGAG AAGAGTCCAG ACCTACAATC TCTACGCTAT
    GCACTTTCTC TTTACACACA AGCAACCGAT ACGCTAATCA AAACCTTCGT CCAGACACAG
    TCTGCTCAGG GATCTGGGGT GGATGATTCT GTTGGGGAAG TTTCTATCCA TGTGGAGTTA
    TTTACTCACC CAGGAACAGG CGAGCACAAG ATCAGCGTAA AGGTTGTAGC GGCCAATGAC
    CTTAAATGGC AGACGTCAGG GATTTTCCGT CCCTTTATTG AGGTAAACAT GATTGGTCCG
    AATCTGAGTG ACAAAAAAAG GAAATTTACC ACCAAGTCCA AGAACAACAG CTGGGCCCCA
    AAATATAACG AGAGCTTCCA ATTTACCCTG GGTAATGAGA CTGGTCCAGA GAGTTATGAG
    CTGCAAGTAT GTGTGAAAGA TTACTGTTTT GCTCGGGAAG ACCGCACAGT GGGAATGGCT
    GTCATGCAAC TGCGAGATCT GGCACAAAGG GGTAGCTGTG CCTGTTGGCT CCCTCTTGGC
    CGCCGCATTC ATATGGATGA GACAGGACTG ACCATCTTGC GCATACTATC ACAAAGAAAC
    AATGATGAGG TGGCCAAAGA GTTTGTCAAA CTCAAATCTG ACACCCGCTC TCCAGAAGAG
    GGGAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa42011
    Clone ID Related Accession (Same CDS sequence) XM_018096807.1
    Accession Version XM_018096807.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5157bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein unc-13 homolog A isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCTTTGC TTTGCGTGGG AGTAAAAAAG GCCACGTTTG ATGGAGCACA AGAGAAGTTT 
    AATGCCTACG TAACCTTGAA GGTGCAAAAT GTGAAGAGCA CAACGATAGC GGTGCGGGGA
    AACCAGCCCT GCTGGGAACA GGATTTCATG TTTGAGATTA ATCGTTTAGA CCTTGGTCTA
    ACAGTGGAGG TCTGGAACAA AGGATTGATC TGGGACACCA TGGTGGGAAC AGTATGGATT
    CCTTTAAGGA CAATCCGACA ATCCAATGAG GAGGGTCCTG GGGAATGGCT GACACTTGAT
    TCACAGGTCA TAATGACAGA TAATGAGATT TGTGGTACCA AAGATCCAAC CTTCCACCGC
    ATCTTACTGG ATACCCGATT TGAGCTTCCT CTAGATATAC CAGAAGAAGA AGCTCGATAC
    TGGGCAAAGA AACTCGAGCA ACTTAATGCA ATGAGGGACC AGGATGATTA TGTTTTTCAA
    GAAGAAATTC CGGAGAATCC TCTTCCTGTC CATGGATCTC AATGTTGTAA CTGGAATTAT
    TTCGGCTGGG GGGAGCAGCA CAATGAGGAT CCCGATAGTG CAGTGGATGA TCGGGATAGT
    GATTACCGCA GTGAAACCAG CAACAGTATC CCGCCCCCCT ACTACTCCAC CTCACAGCCT
    AATGCCTCTG TGCACCAGTA CCCTGTACGA CAGCAACACA GTTCTCATGG ATCTTACACT
    GATTCAGTGC ACAGCTATGA GATGGACTTC TCTGACCATC GTCCAATCAG GCGTTACGAT
    AGCGCAGACT CTGCCACTAA CGGGCGCAGT GATGTGGAGT CTGTCTCCGG TTCGAGCCGC
    CTCACCAGCC GGCAGCACTC TCTGGAGAGC CGGCAGTCCT TAGACCTGTC CGATAGCCCC
    ACTGGAAGTA GTCGATATGC TTCATCAGGG GAGATTAGTC GTGGCTCCTC ACAGCTCAGT
    GATGACTTTG ACCCAGATGA TGACAGCTTG CAAGGTTCAG AAATAGAGGA AGAAAGGGAT
    AGAGACTCTT ATCACTCCTG TCATAGTTCT GTCAGCTGCC GCAAGGACTC GCCAAATTGG
    GAAGGAGAGG AGGAGCGTGA AGTTTATAGT GAAGATGAGG AAGAATATCC TGACAGTAGG
    GGGCAGCTCT CTGACCAGGA GCTGTATGAT GATGACCAGC TTGAGGAAGA AGAGAAAAAA
    GGAAGATTAG CATATGGGCA GGAGGATTCC ATCTATCAGT CATTGGAGGC TGAGGAGAAG
    GAACCCCTGG ATATTCCATT GGAGAAGGAC AGGACACCAG AAGCTGTGCC AGAGGAGGTA
    GTAGAGCCAA CAGTACCAGC AGTCCAGTCT CCCCAAGCTG CAGACAGTTA CAGAGAAGAA
    GAACCTGGAA TGTCCAGGGC CAAATCTAAT TGGTTAAGAG CATTCAACAA GGTGCGCCTG
    CAGCTGCAAG AGGCACGAGG TGAAGGGACT GGGGAGATGT CCAAATCCCT CTGGTTTAAG
    GATGGGCCAG CTGGTGGTCT TATAATTATA GACAGTATGC CAGACATACG AAAGAGGAAA
    CCAATCCCGT TGGTCAGTGA CCTGGCAATG TCACTGGTTC AGTCACGGAA GGCTGGGATT
    ACTTCTGCTC TTGCAACAAG CACCTTAAAC AATGAAGAGC TAAAAAACCA TGTTTACAAA
    AAGACCCTGC AAGCTTTAAT ATACCCAATA TCCAGCACCA CTCCCCACAA CTTTGAAGTT
    TGGACTGCCA CAACTCCTAC CTATTGTTAT GAATGCGAAG GGCTTCTGTG GGGAATCGCT
    CGGCAGGGCA TGCGTTGCAC AGAGTGTGGT GTCAAGTGCC ATGAGAAGTG CCAGGATCTG
    CTGAATGCAG ATTGTCTTCA AAGAGCTGCA GAGAAGAGCT CAAAACATGG TGCAGAAGAC
    CGGACTCAGA ATATTATTAT GGTCTTGAAG GATCGTATGA AGATCCGGGA GAGGAACAAG
    CCAGAAATAT TTGAGCTGAT CCAGGAAGTA TTTGGGGTAT TAAAGGCTAC ACACGCTCAG
    CAGATAAAGG CTGTGAAACA GAGTGTGCTG GATGGTACTT CCAAATGGTC AGCAAAGATC
    AGCATAACTG TTGTATGTGC ACAGGGTCTA CAGGCCAAAG ATAAGACAGG CTCCAGTGAC
    CCATATGTTA CTGTTCAAGT TGGGAAAACC AAGAAAAGAA CCAAAACAAT CTATGGAAAT
    CTTAATCCTG TCTGGGAAGA GAATTTTCAC TTTGAATGCC ACAACTCCTC AGATCGTATT
    AAAGTACGCG TGTGGGATGA AGATGATGAT ATCAAATCTC GTGTGAAGCA GAGATTTAAA
    AGAGAATCAG ATGACTTCCT TGGACAAACA ATTATTGAAG TCAGAACACT TAGCGGAGAG
    ATGGATGTGT GGTATAACTT GGATAAGAGA ACAGATAAAT CCGCTGTCTC TGGGGCAATC
    CGTCTTCATA TAAGTGTGGA AATAAAAGGA GAGGAAAAGG TGGCTCCCTA TCACGTGCAA
    TACACATGTC TACATGAGAA CTTATTCCAT TACCTGACGG ATGTGCAGAA TAATGGTGTG
    GTCAAGATTC CAGATGCAAA AGGAGACGAC GCCTGGAAAG TTTATTTTGA TGAGACAGCC
    CAGGAAATTG TGGATGAATT TGCAATGAGA TATGGAGTGG AGTCCATATA CCAGGCCATG
    ACGCATTTTG CCTGCCTTTC ATCTAAATAT ATGTGTCCAG GAGTACCAGC TGTAATGAGT
    ACTCTTCTTG CCAACATTAA TGCTTACTAT GCGCACACAA CTGCATCTAC TAATGTGTCT
    GCCTCCGACC GATTCTCTGC CTCAAACTTT GGGAAGGAGA GATTTGTAAA GCTGCTTGAC
    CAGTTGCACA ATTCCTTACG CATTGACCTG TCCATGTATC GGAACAACTT CCCTGCTAGC
    AGCCCTGAAA GACTCCAAGA TCTCAAGTCC ACTGTAGATT TACTAACTAG CATAACATTT
    TTCCGGATGA AGGTACAAGA GCTTCAGAGC CCACCACGGG CAAGCCAAGT TGTTAAGGAC
    TGTGTGAAGG CATGCCTTAA CTCAACCTAC GAATATATCT TTAACAACTG CCATGAACTT
    TATAGCCGAG AATATCAAAG TGACCCTGCT AAAAAAGGGG AGGTACCCCC AGAAGAACAG
    GGGCCAAGTA TAAAGAACTT AGATTTTTGG TCCAAACTAA TCACATTGAT TGTGTCTATC
    ATAGAGGAAG ATAAGAACTC ATATACACCT TGTCTAAACC AGTTTCCTCA GGAGCTAAAT
    GTTGGTAAGA TTAGTGCTGA GGTCATGTGG AGTCTCTTTG CTCAAGACAT GAGATATGCT
    ATGGAAGAAC ACGACAAACA CCGCCTGTGT AAGAGTGCAG ACTACATGAA CTTGCATTTT
    AAGGTCAAGT GGTTGTACAA TGAATATGTA ACTGAGCTAC CATCTTTTAA GAGCAAGGTT
    CCAGAATATC CCGCCTGGTT TGAGCCTTTT GTAATTCAGT GGCTGGATGA AAATGAAGAG
    GTCTCCCGGG ATTTTCTGCA TGGAGCACTT GAAAGAGATA AGAAGGATGG GTTCCAGCAG
    ACCTCTGAAC ATGCCCTGTT CTCCTGCTCT GTGGTAGATG TTTTCTCACA ACTTAACCAG
    AGCTTTGAGA TCATTAAGAA GTTGGAGTGT CCAGACCCAC AAATTGTGGG GCATTACATG
    CGTAGATTTG CCAAGACGAT CAGTAATGTT CTGCTGCAGT ATGCTGAAAT TATTTCCAAA
    GATTTTGCAT CTCATTGCTC CAAAGAGAAG AAAGAAGAAA AAGTGCCCTG TATTCTTATG
    AACAATATCC AGCAACTTCG AGTGCAACTA GAAAAAATGT TTGAAGCAAT GGGAGGAAAG
    GAGCTGGATG CAGAAACAAG TGACATTCTT AAAGAGCTGC AGGTGAAACT CAACAATGTC
    CTAGATGAGC TAAGCAGAGT GTTTACCACC AGTTTTCAGG CACAAATTGA GGAGTGTGTG
    AAGCAGATGG GTGACATTCT GAGCCAGGTA AAAGGGACCG GAAATGTACC AGCCAGTAAC
    TGCAGCAGTG TGGCTCAGGA TGCAGATAAT GTATTGCAGC CTATCCTGGA TCCTCTGGAC
    AGCAACCTGA CACTATTTGC CAAGATCTGT GAAAAGACAG TCCTGAAGCG GGTACTAAAA
    GAACTCTGGA AGCTTGTGAT GAATACAATG GAGAAAACGA TTGTCCTTCC ACCACTAACA
    GACCAAACAA TGATAGGAAC CCACCTTATC ATTAATGCAG CCAAGGAACT GGGCCAGTTA
    TCCAAACTAA AGGATCACAT GGTGCGAGAG GAAGCCAAGA GCCTCACCCC TAAGCAGTGT
    GCAGTGGTGG AGCTTGCACT TGATACAATT AAGCAATATT TCCATGCTGG AGGTGTTGGT
    CTCAAGAAGA CATTCCTGGA GAAGAGTCCA GACCTACAAT CTCTACGCTA TGCACTTTCT
    CTTTACACAC AAGCAACCGA TACGCTAATC AAAACCTTCG TCCAGACACA GTCTGCTCAG
    GTTCATGGTG GTAAAGGTAT TAGGTTTACA CTTAATGAAG AGACTTATCC AGAAAAGGGA
    TCTGGGGTGG ATGATTCTGT TGGGGAAGTT TCTATCCATG TGGAGTTATT TACTCACCCA
    GGAACAGGCG AGCACAAGAT CAGCGTAAAG GTTGTAGCGG CCAATGACCT TAAATGGCAG
    ACGTCAGGGA TTTTCCGTCC CTTTATTGAG GTAAACATGA TTGGTCCGAA TCTGAGTGAC
    AAAAAAAGGA AATTTACCAC CAAGTCCAAG AACAACAGCT GGGCCCCAAA ATATAACGAG
    AGCTTCCAAT TTACCCTGGG TAATGAGACT GGTCCAGAGA GTTATGAGCT GCAAGTATGT
    GTGAAAGATT ACTGTTTTGC TCGGGAAGAC CGCACAGTGG GAATGGCTGT CATGCAACTG
    CGAGATCTGG CACAAAGGGG TAGCTGTGCC TGTTGGCTCC CTCTTGGCCG CCGCATTCAT
    ATGGATGAGA CAGGACTGAC CATCTTGCGC ATACTATCAC AAAGAAACAA TGATGAGGTG
    GCCAAAGAGT TTGTCAAACT CAAATCTGAC ACCCGCTCTC CAGAAGAGGG GAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017952296.1
    CDS188..5344
    Translation

    Target ORF information:

    RefSeq Version XM_018096807.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis unc-13 homolog A (unc13a), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018096807.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCTTTGC TTTGCGTGGG AGTAAAAAAG GCCACGTTTG ATGGAGCACA AGAGAAGTTT 
    AATGCCTACG TAACCTTGAA GGTGCAAAAT GTGAAGAGCA CAACGATAGC GGTGCGGGGA
    AACCAGCCCT GCTGGGAACA GGATTTCATG TTTGAGATTA ATCGTTTAGA CCTTGGTCTA
    ACAGTGGAGG TCTGGAACAA AGGATTGATC TGGGACACCA TGGTGGGAAC AGTATGGATT
    CCTTTAAGGA CAATCCGACA ATCCAATGAG GAGGGTCCTG GGGAATGGCT GACACTTGAT
    TCACAGGTCA TAATGACAGA TAATGAGATT TGTGGTACCA AAGATCCAAC CTTCCACCGC
    ATCTTACTGG ATACCCGATT TGAGCTTCCT CTAGATATAC CAGAAGAAGA AGCTCGATAC
    TGGGCAAAGA AACTCGAGCA ACTTAATGCA ATGAGGGACC AGGATGATTA TGTTTTTCAA
    GAAGAAATTC CGGAGAATCC TCTTCCTGTC CATGGATCTC AATGTTGTAA CTGGAATTAT
    TTCGGCTGGG GGGAGCAGCA CAATGAGGAT CCCGATAGTG CAGTGGATGA TCGGGATAGT
    GATTACCGCA GTGAAACCAG CAACAGTATC CCGCCCCCCT ACTACTCCAC CTCACAGCCT
    AATGCCTCTG TGCACCAGTA CCCTGTACGA CAGCAACACA GTTCTCATGG ATCTTACACT
    GATTCAGTGC ACAGCTATGA GATGGACTTC TCTGACCATC GTCCAATCAG GCGTTACGAT
    AGCGCAGACT CTGCCACTAA CGGGCGCAGT GATGTGGAGT CTGTCTCCGG TTCGAGCCGC
    CTCACCAGCC GGCAGCACTC TCTGGAGAGC CGGCAGTCCT TAGACCTGTC CGATAGCCCC
    ACTGGAAGTA GTCGATATGC TTCATCAGGG GAGATTAGTC GTGGCTCCTC ACAGCTCAGT
    GATGACTTTG ACCCAGATGA TGACAGCTTG CAAGGTTCAG AAATAGAGGA AGAAAGGGAT
    AGAGACTCTT ATCACTCCTG TCATAGTTCT GTCAGCTGCC GCAAGGACTC GCCAAATTGG
    GAAGGAGAGG AGGAGCGTGA AGTTTATAGT GAAGATGAGG AAGAATATCC TGACAGTAGG
    GGGCAGCTCT CTGACCAGGA GCTGTATGAT GATGACCAGC TTGAGGAAGA AGAGAAAAAA
    GGAAGATTAG CATATGGGCA GGAGGATTCC ATCTATCAGT CATTGGAGGC TGAGGAGAAG
    GAACCCCTGG ATATTCCATT GGAGAAGGAC AGGACACCAG AAGCTGTGCC AGAGGAGGTA
    GTAGAGCCAA CAGTACCAGC AGTCCAGTCT CCCCAAGCTG CAGACAGTTA CAGAGAAGAA
    GAACCTGGAA TGTCCAGGGC CAAATCTAAT TGGTTAAGAG CATTCAACAA GGTGCGCCTG
    CAGCTGCAAG AGGCACGAGG TGAAGGGACT GGGGAGATGT CCAAATCCCT CTGGTTTAAG
    GATGGGCCAG CTGGTGGTCT TATAATTATA GACAGTATGC CAGACATACG AAAGAGGAAA
    CCAATCCCGT TGGTCAGTGA CCTGGCAATG TCACTGGTTC AGTCACGGAA GGCTGGGATT
    ACTTCTGCTC TTGCAACAAG CACCTTAAAC AATGAAGAGC TAAAAAACCA TGTTTACAAA
    AAGACCCTGC AAGCTTTAAT ATACCCAATA TCCAGCACCA CTCCCCACAA CTTTGAAGTT
    TGGACTGCCA CAACTCCTAC CTATTGTTAT GAATGCGAAG GGCTTCTGTG GGGAATCGCT
    CGGCAGGGCA TGCGTTGCAC AGAGTGTGGT GTCAAGTGCC ATGAGAAGTG CCAGGATCTG
    CTGAATGCAG ATTGTCTTCA AAGAGCTGCA GAGAAGAGCT CAAAACATGG TGCAGAAGAC
    CGGACTCAGA ATATTATTAT GGTCTTGAAG GATCGTATGA AGATCCGGGA GAGGAACAAG
    CCAGAAATAT TTGAGCTGAT CCAGGAAGTA TTTGGGGTAT TAAAGGCTAC ACACGCTCAG
    CAGATAAAGG CTGTGAAACA GAGTGTGCTG GATGGTACTT CCAAATGGTC AGCAAAGATC
    AGCATAACTG TTGTATGTGC ACAGGGTCTA CAGGCCAAAG ATAAGACAGG CTCCAGTGAC
    CCATATGTTA CTGTTCAAGT TGGGAAAACC AAGAAAAGAA CCAAAACAAT CTATGGAAAT
    CTTAATCCTG TCTGGGAAGA GAATTTTCAC TTTGAATGCC ACAACTCCTC AGATCGTATT
    AAAGTACGCG TGTGGGATGA AGATGATGAT ATCAAATCTC GTGTGAAGCA GAGATTTAAA
    AGAGAATCAG ATGACTTCCT TGGACAAACA ATTATTGAAG TCAGAACACT TAGCGGAGAG
    ATGGATGTGT GGTATAACTT GGATAAGAGA ACAGATAAAT CCGCTGTCTC TGGGGCAATC
    CGTCTTCATA TAAGTGTGGA AATAAAAGGA GAGGAAAAGG TGGCTCCCTA TCACGTGCAA
    TACACATGTC TACATGAGAA CTTATTCCAT TACCTGACGG ATGTGCAGAA TAATGGTGTG
    GTCAAGATTC CAGATGCAAA AGGAGACGAC GCCTGGAAAG TTTATTTTGA TGAGACAGCC
    CAGGAAATTG TGGATGAATT TGCAATGAGA TATGGAGTGG AGTCCATATA CCAGGCCATG
    ACGCATTTTG CCTGCCTTTC ATCTAAATAT ATGTGTCCAG GAGTACCAGC TGTAATGAGT
    ACTCTTCTTG CCAACATTAA TGCTTACTAT GCGCACACAA CTGCATCTAC TAATGTGTCT
    GCCTCCGACC GATTCTCTGC CTCAAACTTT GGGAAGGAGA GATTTGTAAA GCTGCTTGAC
    CAGTTGCACA ATTCCTTACG CATTGACCTG TCCATGTATC GGAACAACTT CCCTGCTAGC
    AGCCCTGAAA GACTCCAAGA TCTCAAGTCC ACTGTAGATT TACTAACTAG CATAACATTT
    TTCCGGATGA AGGTACAAGA GCTTCAGAGC CCACCACGGG CAAGCCAAGT TGTTAAGGAC
    TGTGTGAAGG CATGCCTTAA CTCAACCTAC GAATATATCT TTAACAACTG CCATGAACTT
    TATAGCCGAG AATATCAAAG TGACCCTGCT AAAAAAGGGG AGGTACCCCC AGAAGAACAG
    GGGCCAAGTA TAAAGAACTT AGATTTTTGG TCCAAACTAA TCACATTGAT TGTGTCTATC
    ATAGAGGAAG ATAAGAACTC ATATACACCT TGTCTAAACC AGTTTCCTCA GGAGCTAAAT
    GTTGGTAAGA TTAGTGCTGA GGTCATGTGG AGTCTCTTTG CTCAAGACAT GAGATATGCT
    ATGGAAGAAC ACGACAAACA CCGCCTGTGT AAGAGTGCAG ACTACATGAA CTTGCATTTT
    AAGGTCAAGT GGTTGTACAA TGAATATGTA ACTGAGCTAC CATCTTTTAA GAGCAAGGTT
    CCAGAATATC CCGCCTGGTT TGAGCCTTTT GTAATTCAGT GGCTGGATGA AAATGAAGAG
    GTCTCCCGGG ATTTTCTGCA TGGAGCACTT GAAAGAGATA AGAAGGATGG GTTCCAGCAG
    ACCTCTGAAC ATGCCCTGTT CTCCTGCTCT GTGGTAGATG TTTTCTCACA ACTTAACCAG
    AGCTTTGAGA TCATTAAGAA GTTGGAGTGT CCAGACCCAC AAATTGTGGG GCATTACATG
    CGTAGATTTG CCAAGACGAT CAGTAATGTT CTGCTGCAGT ATGCTGAAAT TATTTCCAAA
    GATTTTGCAT CTCATTGCTC CAAAGAGAAG AAAGAAGAAA AAGTGCCCTG TATTCTTATG
    AACAATATCC AGCAACTTCG AGTGCAACTA GAAAAAATGT TTGAAGCAAT GGGAGGAAAG
    GAGCTGGATG CAGAAACAAG TGACATTCTT AAAGAGCTGC AGGTGAAACT CAACAATGTC
    CTAGATGAGC TAAGCAGAGT GTTTACCACC AGTTTTCAGG CACAAATTGA GGAGTGTGTG
    AAGCAGATGG GTGACATTCT GAGCCAGGTA AAAGGGACCG GAAATGTACC AGCCAGTAAC
    TGCAGCAGTG TGGCTCAGGA TGCAGATAAT GTATTGCAGC CTATCCTGGA TCCTCTGGAC
    AGCAACCTGA CACTATTTGC CAAGATCTGT GAAAAGACAG TCCTGAAGCG GGTACTAAAA
    GAACTCTGGA AGCTTGTGAT GAATACAATG GAGAAAACGA TTGTCCTTCC ACCACTAACA
    GACCAAACAA TGATAGGAAC CCACCTTATC ATTAATGCAG CCAAGGAACT GGGCCAGTTA
    TCCAAACTAA AGGATCACAT GGTGCGAGAG GAAGCCAAGA GCCTCACCCC TAAGCAGTGT
    GCAGTGGTGG AGCTTGCACT TGATACAATT AAGCAATATT TCCATGCTGG AGGTGTTGGT
    CTCAAGAAGA CATTCCTGGA GAAGAGTCCA GACCTACAAT CTCTACGCTA TGCACTTTCT
    CTTTACACAC AAGCAACCGA TACGCTAATC AAAACCTTCG TCCAGACACA GTCTGCTCAG
    GTTCATGGTG GTAAAGGTAT TAGGTTTACA CTTAATGAAG AGACTTATCC AGAAAAGGGA
    TCTGGGGTGG ATGATTCTGT TGGGGAAGTT TCTATCCATG TGGAGTTATT TACTCACCCA
    GGAACAGGCG AGCACAAGAT CAGCGTAAAG GTTGTAGCGG CCAATGACCT TAAATGGCAG
    ACGTCAGGGA TTTTCCGTCC CTTTATTGAG GTAAACATGA TTGGTCCGAA TCTGAGTGAC
    AAAAAAAGGA AATTTACCAC CAAGTCCAAG AACAACAGCT GGGCCCCAAA ATATAACGAG
    AGCTTCCAAT TTACCCTGGG TAATGAGACT GGTCCAGAGA GTTATGAGCT GCAAGTATGT
    GTGAAAGATT ACTGTTTTGC TCGGGAAGAC CGCACAGTGG GAATGGCTGT CATGCAACTG
    CGAGATCTGG CACAAAGGGG TAGCTGTGCC TGTTGGCTCC CTCTTGGCCG CCGCATTCAT
    ATGGATGAGA CAGGACTGAC CATCTTGCGC ATACTATCAC AAAGAAACAA TGATGAGGTG
    GCCAAAGAGT TTGTCAAACT CAAATCTGAC ACCCGCTCTC CAGAAGAGGG GAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa42012
    Clone ID Related Accession (Same CDS sequence) XM_018096811.1
    Accession Version XM_018096811.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5151bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein unc-13 homolog A isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCTTTGC TTTGCGTGGG AGTAAAAAAG GCCACGTTTG ATGGAGCACA AGAGAAGTTT 
    AATGCCTACG TAACCTTGAA GGTGCAAAAT GTGAAGAGCA CAACGATAGC GGTGCGGGGA
    AACCAGCCCT GCTGGGAACA GGATTTCATG TTTGAGATTA ATCGTTTAGA CCTTGGTCTA
    ACAGTGGAGG TCTGGAACAA AGGATTGATC TGGGACACCA TGGTGGGAAC AGTATGGATT
    CCTTTAAGGA CAATCCGACA ATCCAATGAG GAGGGTCCTG GGGAATGGCT GACACTTGAT
    TCACAGGTCA TAATGACAGA TAATGAGATT TGTGGTACCA AAGATCCAAC CTTCCACCGC
    ATCTTACTGG ATACCCGATT TGAGCTTCCT CTAGATATAC CAGAAGAAGA AGCTCGATAC
    TGGGCAAAGA AACTCGAGCA ACTTAATGCA ATGAGGGACC AGGATGATTA TGTTTTTCAA
    GAAGAAATTC CGGAGAATCC TCTTCCTGTC CATGGATCTC AATGTTGTAA CTGGAATTAT
    TTCGGCTGGG GGGAGCAGCA CAATGAGGAT CCCGATAGTG CAGTGGATGA TCGGGATAGT
    GATTACCGCA GTGAAACCAG CAACAGTATC CCGCCCCCCT ACTACTCCAC CTCACAGCCT
    AATGCCTCTG TGCACCAGTA CCCTGTACGA CAGCAACACA GTTCTCATGG ATCTTACACT
    GATTCAGTGC ACAGCTATGA GATGGACTTC TCTGACCATC GTCCAATCAG GCGTTACGAT
    AGCGCAGACT CTGCCACTAA CGGGCGCAGT GATGTGGAGT CTGTCTCCGG TTCGAGCCGC
    CTCACCAGCC GGCAGCACTC TCTGGAGAGC CGGCAGTCCT TAGACCTGTC CGATAGCCCC
    ACTGGAAGTA GTCGATATGC TTCATCAGGG GAGATTAGTC GTGGCTCCTC ACAGCTCAGT
    GATGACTTTG ACCCAGATGA TGACAGCTTG CAAGGTTCAG AAATAGAGGA AGAAAGGGAT
    AGAGACTCTT ATCACTCCTG TCATAGTTCT GTCAGCTGCC GCAAGGACTC GCCAAATTGG
    GAAGGAGAGG AGGAGCGTGA AGTTTATAGT GAAGATGAGG AAGAATATCC TGACAGTAGG
    GGGCAGCTCT CTGACCAGGA GCTGTATGAT GATGACCAGC TTGAGGAAGA AGAGAAAAAA
    GGAAGATTAG CATATGGGCA GGAGGATTCC ATCTATCAGT CATTGGAGGC TGAGGAGAAG
    GAACCCCTGG ATATTCCATT GGAGAAGGAC AGGACACCAG AAGCTGTGCC AGAGGAGGTA
    GTAGAGCCAA CAGTACCAGC AGTCCAGTCT CCCCAAGCTG CAGACAGTTA CAGAGAAGAA
    GAACCTGGAA TGTCCAGGGC CAAATCTAAT TGGTTAAGAG CATTCAACAA GGTGCGCCTG
    CAGCTGCAAG AGGCACGAGG TGAAGGGACT GGGGAGATGT CCAAATCCCT CTGGTTTAAG
    GATGGGCCAG CTGGTGGTCT TATAATTATA GACAGTATGC CAGACATACG AAAGAGGAAA
    CCAATCCCGT TGGTCAGTGA CCTGGCAATG TCACTGGTTC AGTCACGGAA GGCTGGGATT
    ACTTCTGCTC TTGCAACAAG CACCTTAAAC AATGAAGAGC TAAAAAACCA TGTTTACAAA
    AAGACCCTGC AAGCTTTAAT ATACCCAATA TCCAGCACCA CTCCCCACAA CTTTGAAGTT
    TGGACTGCCA CAACTCCTAC CTATTGTTAT GAATGCGAAG GGCTTCTGTG GGGAATCGCT
    CGGCAGGGCA TGCGTTGCAC AGAGTGTGGT GTCAAGTGCC ATGAGAAGTG CCAGGATCTG
    CTGAATGCAG ATTGTCTTCA AAGAGCTGCA GAGAAGAGCT CAAAACATGG TGCAGAAGAC
    CGGACTCAGA ATATTATTAT GGTCTTGAAG GATCGTATGA AGATCCGGGA GAGGAACAAG
    CCAGAAATAT TTGAGCTGAT CCAGGAAGTA TTTGGGGTAT TAAAGGCTAC ACACGCTCAG
    CAGATAAAGG CTGTGAAACA GAGTGTGCTG GATGGTACTT CCAAATGGTC AGCAAAGATC
    AGCATAACTG TTGTATGTGC ACAGGGTCTA CAGGCCAAAG ATAAGACAGG CTCCAGTGAC
    CCATATGTTA CTGTTCAAGT TGGGAAAACC AAGAAAAGAA CCAAAACAAT CTATGGAAAT
    CTTAATCCTG TCTGGGAAGA GAATTTTCAC TTTGAATGCC ACAACTCCTC AGATCGTATT
    AAAGTACGCG TGTGGGATGA AGATGATGAT ATCAAATCTC GTGTGAAGCA GAGATTTAAA
    AGAGAATCAG ATGACTTCCT TGGACAAACA ATTATTGAAG TCAGAACACT TAGCGGAGAG
    ATGGATGTGT GGTATAACTT GGATAAGAGA ACAGATAAAT CCGCTGTCTC TGGGGCAATC
    CGTCTTCATA TAAGTGTGGA AATAAAAGGA GAGGAAAAGG TGGCTCCCTA TCACGTGCAA
    TACACATGTC TACATGAGAA CTTATTCCAT TACCTGACGG ATGTGCAGAA TAATGGTGTG
    GTCAAGATTC CAGATGCAAA AGGAGACGAC GCCTGGAAAG TTTATTTTGA TGAGACAGCC
    CAGGAAATTG TGGATGAATT TGCAATGAGA TATGGAGTGG AGTCCATATA CCAGGCCATG
    ACGCATTTTG CCTGCCTTTC ATCTAAATAT ATGTGTCCAG GAGTACCAGC TGTAATGAGT
    ACTCTTCTTG CCAACATTAA TGCTTACTAT GCGCACACAA CTGCATCTAC TAATGTGTCT
    GCCTCCGACC GATTCTCTGC CTCAAACTTT GGGAAGGAGA GATTTGTAAA GCTGCTTGAC
    CAGTTGCACA ATTCCTTACG CATTGACCTG TCCATGTATC GGAACAACTT CCCTGCTAGC
    AGCCCTGAAA GACTCCAAGA TCTCAAGTCC ACTGTAGATT TACTAACTAG CATAACATTT
    TTCCGGATGA AGGTACAAGA GCTTCAGAGC CCACCACGGG CAAGCCAAGT TGTTAAGGAC
    TGTGTGAAGG CATGCCTTAA CTCAACCTAC GAATATATCT TTAACAACTG CCATGAACTT
    TATAGCCGAG AATATCAAAG TGACCCTGCT AAAAAAGGGG AGGTACCCCC AGAAGAACAG
    GGGCCAAGTA TAAAGAACTT AGATTTTTGG TCCAAACTAA TCACATTGAT TGTGTCTATC
    ATAGAGGAAG ATAAGAACTC ATATACACCT TGTCTAAACC AGTTTCCTCA GGAGCTAAAT
    GTTGGTAAGA TTAGTGCTGA GGTCATGTGG AGTCTCTTTG CTCAAGACAT GAGATATGCT
    ATGGAAGAAC ACGACAAACA CCGCCTGTGT AAGAGTGCAG ACTACATGAA CTTGCATTTT
    AAGGTCAAGT GGTTGTACAA TGAATATGTA ACTGAGCTAC CATCTTTTAA GAGCAAGGTT
    CCAGAATATC CCGCCTGGTT TGAGCCTTTT GTAATTCAGT GGCTGGATGA AAATGAAGAG
    GTCTCCCGGG ATTTTCTGCA TGGAGCACTT GAAAGAGATA AGAAGGATGG GTTCCAGCAG
    ACCTCTGAAC ATGCCCTGTT CTCCTGCTCT GTGGTAGATG TTTTCTCACA ACTTAACCAG
    AGCTTTGAGA TCATTAAGAA GTTGGAGTGT CCAGACCCAC AAATTGTGGG GCATTACATG
    CGTAGATTTG CCAAGACGAT CAGTAATGTT CTGCTGCAGT ATGCTGAAAT TATTTCCAAA
    GATTTTGCAT CTCATTGCTC CAAAGAGAAG AAAGAAGAAA AAGTGCCCTG TATTCTTATG
    AACAATATCC AGCAACTTCG AGTGCAACTA GAAAAAATGT TTGAAGCAAT GGGAGGAAAG
    GAGCTGGATG CAGAAACAAG TGACATTCTT AAAGAGCTGC AGGTGAAACT CAACAATGTC
    CTAGATGAGC TAAGCAGAGT GTTTACCACC AGTTTTCAGG CACAAATTGA GGAGTGTGTG
    AAGCAGATGG GTGACATTCT GAGCCAGGTA AAAGGGACCG GAAATGTACC AGCCAGTAAC
    TGCAGCAGTG TGGCTCAGGA TGCAGATAAT GTATTGCAGC CTATCCTGGA TCCTCTGGAC
    AGCAACCTGA CACTATTTGC CAAGATCTGT GAAAAGACAG TCCTGAAGCG GGTACTAAAA
    GAACTCTGGA AGCTTGTGAT GAATACAATG GAGAAAACGA TTGTCCTTCC ACCACTAACA
    GACCAAACAG GAACCCACCT TATCATTAAT GCAGCCAAGG AACTGGGCCA GTTATCCAAA
    CTAAAGGATC ACATGGTGCG AGAGGAAGCC AAGAGCCTCA CCCCTAAGCA GTGTGCAGTG
    GTGGAGCTTG CACTTGATAC AATTAAGCAA TATTTCCATG CTGGAGGTGT TGGTCTCAAG
    AAGACATTCC TGGAGAAGAG TCCAGACCTA CAATCTCTAC GCTATGCACT TTCTCTTTAC
    ACACAAGCAA CCGATACGCT AATCAAAACC TTCGTCCAGA CACAGTCTGC TCAGGTTCAT
    GGTGGTAAAG GTATTAGGTT TACACTTAAT GAAGAGACTT ATCCAGAAAA GGGATCTGGG
    GTGGATGATT CTGTTGGGGA AGTTTCTATC CATGTGGAGT TATTTACTCA CCCAGGAACA
    GGCGAGCACA AGATCAGCGT AAAGGTTGTA GCGGCCAATG ACCTTAAATG GCAGACGTCA
    GGGATTTTCC GTCCCTTTAT TGAGGTAAAC ATGATTGGTC CGAATCTGAG TGACAAAAAA
    AGGAAATTTA CCACCAAGTC CAAGAACAAC AGCTGGGCCC CAAAATATAA CGAGAGCTTC
    CAATTTACCC TGGGTAATGA GACTGGTCCA GAGAGTTATG AGCTGCAAGT ATGTGTGAAA
    GATTACTGTT TTGCTCGGGA AGACCGCACA GTGGGAATGG CTGTCATGCA ACTGCGAGAT
    CTGGCACAAA GGGGTAGCTG TGCCTGTTGG CTCCCTCTTG GCCGCCGCAT TCATATGGAT
    GAGACAGGAC TGACCATCTT GCGCATACTA TCACAAAGAA ACAATGATGA GGTGGCCAAA
    GAGTTTGTCA AACTCAAATC TGACACCCGC TCTCCAGAAG AGGGGAGCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017952300.1
    CDS188..5338
    Translation

    Target ORF information:

    RefSeq Version XM_018096811.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis unc-13 homolog A (unc13a), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018096811.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCTTTGC TTTGCGTGGG AGTAAAAAAG GCCACGTTTG ATGGAGCACA AGAGAAGTTT 
    AATGCCTACG TAACCTTGAA GGTGCAAAAT GTGAAGAGCA CAACGATAGC GGTGCGGGGA
    AACCAGCCCT GCTGGGAACA GGATTTCATG TTTGAGATTA ATCGTTTAGA CCTTGGTCTA
    ACAGTGGAGG TCTGGAACAA AGGATTGATC TGGGACACCA TGGTGGGAAC AGTATGGATT
    CCTTTAAGGA CAATCCGACA ATCCAATGAG GAGGGTCCTG GGGAATGGCT GACACTTGAT
    TCACAGGTCA TAATGACAGA TAATGAGATT TGTGGTACCA AAGATCCAAC CTTCCACCGC
    ATCTTACTGG ATACCCGATT TGAGCTTCCT CTAGATATAC CAGAAGAAGA AGCTCGATAC
    TGGGCAAAGA AACTCGAGCA ACTTAATGCA ATGAGGGACC AGGATGATTA TGTTTTTCAA
    GAAGAAATTC CGGAGAATCC TCTTCCTGTC CATGGATCTC AATGTTGTAA CTGGAATTAT
    TTCGGCTGGG GGGAGCAGCA CAATGAGGAT CCCGATAGTG CAGTGGATGA TCGGGATAGT
    GATTACCGCA GTGAAACCAG CAACAGTATC CCGCCCCCCT ACTACTCCAC CTCACAGCCT
    AATGCCTCTG TGCACCAGTA CCCTGTACGA CAGCAACACA GTTCTCATGG ATCTTACACT
    GATTCAGTGC ACAGCTATGA GATGGACTTC TCTGACCATC GTCCAATCAG GCGTTACGAT
    AGCGCAGACT CTGCCACTAA CGGGCGCAGT GATGTGGAGT CTGTCTCCGG TTCGAGCCGC
    CTCACCAGCC GGCAGCACTC TCTGGAGAGC CGGCAGTCCT TAGACCTGTC CGATAGCCCC
    ACTGGAAGTA GTCGATATGC TTCATCAGGG GAGATTAGTC GTGGCTCCTC ACAGCTCAGT
    GATGACTTTG ACCCAGATGA TGACAGCTTG CAAGGTTCAG AAATAGAGGA AGAAAGGGAT
    AGAGACTCTT ATCACTCCTG TCATAGTTCT GTCAGCTGCC GCAAGGACTC GCCAAATTGG
    GAAGGAGAGG AGGAGCGTGA AGTTTATAGT GAAGATGAGG AAGAATATCC TGACAGTAGG
    GGGCAGCTCT CTGACCAGGA GCTGTATGAT GATGACCAGC TTGAGGAAGA AGAGAAAAAA
    GGAAGATTAG CATATGGGCA GGAGGATTCC ATCTATCAGT CATTGGAGGC TGAGGAGAAG
    GAACCCCTGG ATATTCCATT GGAGAAGGAC AGGACACCAG AAGCTGTGCC AGAGGAGGTA
    GTAGAGCCAA CAGTACCAGC AGTCCAGTCT CCCCAAGCTG CAGACAGTTA CAGAGAAGAA
    GAACCTGGAA TGTCCAGGGC CAAATCTAAT TGGTTAAGAG CATTCAACAA GGTGCGCCTG
    CAGCTGCAAG AGGCACGAGG TGAAGGGACT GGGGAGATGT CCAAATCCCT CTGGTTTAAG
    GATGGGCCAG CTGGTGGTCT TATAATTATA GACAGTATGC CAGACATACG AAAGAGGAAA
    CCAATCCCGT TGGTCAGTGA CCTGGCAATG TCACTGGTTC AGTCACGGAA GGCTGGGATT
    ACTTCTGCTC TTGCAACAAG CACCTTAAAC AATGAAGAGC TAAAAAACCA TGTTTACAAA
    AAGACCCTGC AAGCTTTAAT ATACCCAATA TCCAGCACCA CTCCCCACAA CTTTGAAGTT
    TGGACTGCCA CAACTCCTAC CTATTGTTAT GAATGCGAAG GGCTTCTGTG GGGAATCGCT
    CGGCAGGGCA TGCGTTGCAC AGAGTGTGGT GTCAAGTGCC ATGAGAAGTG CCAGGATCTG
    CTGAATGCAG ATTGTCTTCA AAGAGCTGCA GAGAAGAGCT CAAAACATGG TGCAGAAGAC
    CGGACTCAGA ATATTATTAT GGTCTTGAAG GATCGTATGA AGATCCGGGA GAGGAACAAG
    CCAGAAATAT TTGAGCTGAT CCAGGAAGTA TTTGGGGTAT TAAAGGCTAC ACACGCTCAG
    CAGATAAAGG CTGTGAAACA GAGTGTGCTG GATGGTACTT CCAAATGGTC AGCAAAGATC
    AGCATAACTG TTGTATGTGC ACAGGGTCTA CAGGCCAAAG ATAAGACAGG CTCCAGTGAC
    CCATATGTTA CTGTTCAAGT TGGGAAAACC AAGAAAAGAA CCAAAACAAT CTATGGAAAT
    CTTAATCCTG TCTGGGAAGA GAATTTTCAC TTTGAATGCC ACAACTCCTC AGATCGTATT
    AAAGTACGCG TGTGGGATGA AGATGATGAT ATCAAATCTC GTGTGAAGCA GAGATTTAAA
    AGAGAATCAG ATGACTTCCT TGGACAAACA ATTATTGAAG TCAGAACACT TAGCGGAGAG
    ATGGATGTGT GGTATAACTT GGATAAGAGA ACAGATAAAT CCGCTGTCTC TGGGGCAATC
    CGTCTTCATA TAAGTGTGGA AATAAAAGGA GAGGAAAAGG TGGCTCCCTA TCACGTGCAA
    TACACATGTC TACATGAGAA CTTATTCCAT TACCTGACGG ATGTGCAGAA TAATGGTGTG
    GTCAAGATTC CAGATGCAAA AGGAGACGAC GCCTGGAAAG TTTATTTTGA TGAGACAGCC
    CAGGAAATTG TGGATGAATT TGCAATGAGA TATGGAGTGG AGTCCATATA CCAGGCCATG
    ACGCATTTTG CCTGCCTTTC ATCTAAATAT ATGTGTCCAG GAGTACCAGC TGTAATGAGT
    ACTCTTCTTG CCAACATTAA TGCTTACTAT GCGCACACAA CTGCATCTAC TAATGTGTCT
    GCCTCCGACC GATTCTCTGC CTCAAACTTT GGGAAGGAGA GATTTGTAAA GCTGCTTGAC
    CAGTTGCACA ATTCCTTACG CATTGACCTG TCCATGTATC GGAACAACTT CCCTGCTAGC
    AGCCCTGAAA GACTCCAAGA TCTCAAGTCC ACTGTAGATT TACTAACTAG CATAACATTT
    TTCCGGATGA AGGTACAAGA GCTTCAGAGC CCACCACGGG CAAGCCAAGT TGTTAAGGAC
    TGTGTGAAGG CATGCCTTAA CTCAACCTAC GAATATATCT TTAACAACTG CCATGAACTT
    TATAGCCGAG AATATCAAAG TGACCCTGCT AAAAAAGGGG AGGTACCCCC AGAAGAACAG
    GGGCCAAGTA TAAAGAACTT AGATTTTTGG TCCAAACTAA TCACATTGAT TGTGTCTATC
    ATAGAGGAAG ATAAGAACTC ATATACACCT TGTCTAAACC AGTTTCCTCA GGAGCTAAAT
    GTTGGTAAGA TTAGTGCTGA GGTCATGTGG AGTCTCTTTG CTCAAGACAT GAGATATGCT
    ATGGAAGAAC ACGACAAACA CCGCCTGTGT AAGAGTGCAG ACTACATGAA CTTGCATTTT
    AAGGTCAAGT GGTTGTACAA TGAATATGTA ACTGAGCTAC CATCTTTTAA GAGCAAGGTT
    CCAGAATATC CCGCCTGGTT TGAGCCTTTT GTAATTCAGT GGCTGGATGA AAATGAAGAG
    GTCTCCCGGG ATTTTCTGCA TGGAGCACTT GAAAGAGATA AGAAGGATGG GTTCCAGCAG
    ACCTCTGAAC ATGCCCTGTT CTCCTGCTCT GTGGTAGATG TTTTCTCACA ACTTAACCAG
    AGCTTTGAGA TCATTAAGAA GTTGGAGTGT CCAGACCCAC AAATTGTGGG GCATTACATG
    CGTAGATTTG CCAAGACGAT CAGTAATGTT CTGCTGCAGT ATGCTGAAAT TATTTCCAAA
    GATTTTGCAT CTCATTGCTC CAAAGAGAAG AAAGAAGAAA AAGTGCCCTG TATTCTTATG
    AACAATATCC AGCAACTTCG AGTGCAACTA GAAAAAATGT TTGAAGCAAT GGGAGGAAAG
    GAGCTGGATG CAGAAACAAG TGACATTCTT AAAGAGCTGC AGGTGAAACT CAACAATGTC
    CTAGATGAGC TAAGCAGAGT GTTTACCACC AGTTTTCAGG CACAAATTGA GGAGTGTGTG
    AAGCAGATGG GTGACATTCT GAGCCAGGTA AAAGGGACCG GAAATGTACC AGCCAGTAAC
    TGCAGCAGTG TGGCTCAGGA TGCAGATAAT GTATTGCAGC CTATCCTGGA TCCTCTGGAC
    AGCAACCTGA CACTATTTGC CAAGATCTGT GAAAAGACAG TCCTGAAGCG GGTACTAAAA
    GAACTCTGGA AGCTTGTGAT GAATACAATG GAGAAAACGA TTGTCCTTCC ACCACTAACA
    GACCAAACAG GAACCCACCT TATCATTAAT GCAGCCAAGG AACTGGGCCA GTTATCCAAA
    CTAAAGGATC ACATGGTGCG AGAGGAAGCC AAGAGCCTCA CCCCTAAGCA GTGTGCAGTG
    GTGGAGCTTG CACTTGATAC AATTAAGCAA TATTTCCATG CTGGAGGTGT TGGTCTCAAG
    AAGACATTCC TGGAGAAGAG TCCAGACCTA CAATCTCTAC GCTATGCACT TTCTCTTTAC
    ACACAAGCAA CCGATACGCT AATCAAAACC TTCGTCCAGA CACAGTCTGC TCAGGTTCAT
    GGTGGTAAAG GTATTAGGTT TACACTTAAT GAAGAGACTT ATCCAGAAAA GGGATCTGGG
    GTGGATGATT CTGTTGGGGA AGTTTCTATC CATGTGGAGT TATTTACTCA CCCAGGAACA
    GGCGAGCACA AGATCAGCGT AAAGGTTGTA GCGGCCAATG ACCTTAAATG GCAGACGTCA
    GGGATTTTCC GTCCCTTTAT TGAGGTAAAC ATGATTGGTC CGAATCTGAG TGACAAAAAA
    AGGAAATTTA CCACCAAGTC CAAGAACAAC AGCTGGGCCC CAAAATATAA CGAGAGCTTC
    CAATTTACCC TGGGTAATGA GACTGGTCCA GAGAGTTATG AGCTGCAAGT ATGTGTGAAA
    GATTACTGTT TTGCTCGGGA AGACCGCACA GTGGGAATGG CTGTCATGCA ACTGCGAGAT
    CTGGCACAAA GGGGTAGCTG TGCCTGTTGG CTCCCTCTTG GCCGCCGCAT TCATATGGAT
    GAGACAGGAC TGACCATCTT GCGCATACTA TCACAAAGAA ACAATGATGA GGTGGCCAAA
    GAGTTTGTCA AACTCAAATC TGACACCCGC TCTCCAGAAG AGGGGAGCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa42013
    Clone ID Related Accession (Same CDS sequence) XM_018096818.1
    Accession Version XM_018096818.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5094bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein unc-13 homolog A isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCTTTGC TTTGCGTGGG AGTAAAAAAG GCCACGTTTG ATGGAGCACA AGAGAAGTTT 
    AATGCCTACG TAACCTTGAA GGTGCAAAAT GTGAAGAGCA CAACGATAGC GGTGCGGGGA
    AACCAGCCCT GCTGGGAACA GGATTTCATG TTTGAGATTA ATCGTTTAGA CCTTGGTCTA
    ACAGTGGAGG TCTGGAACAA AGGATTGATC TGGGACACCA TGGTGGGAAC AGTATGGATT
    CCTTTAAGGA CAATCCGACA ATCCAATGAG GAGGGTCCTG GGGAATGGCT GACACTTGAT
    TCACAGGTCA TAATGACAGA TAATGAGATT TGTGGTACCA AAGATCCAAC CTTCCACCGC
    ATCTTACTGG ATACCCGATT TGAGCTTCCT CTAGATATAC CAGAAGAAGA AGCTCGATAC
    TGGGCAAAGA AACTCGAGCA ACTTAATGCA ATGAGGGACC AGGATGATTA TGTTTTTCAA
    GAAGAAATTC CGGAGAATCC TCTTCCTGTC CATGGATCTC AATGTTGTAA CTGGAATTAT
    TTCGGCTGGG GGGAGCAGCA CAATGAGGAT CCCGATAGTG CAGTGGATGA TCGGGATAGT
    GATTACCGCA GTGAAACCAG CAACAGTATC CCGCCCCCCT ACTACTCCAC CTCACAGCCT
    AATGCCTCTG TGCACCAGTA CCCTGTACGA CAGCAACACA GTTCTCATGG ATCTTACACT
    GATTCAGTGC ACAGCTATGA GATGGACTTC TCTGACCATC GTCCAATCAG GCGTTACGAT
    AGCGCAGACT CTGCCACTAA CGGGCGCAGT GATGTGGAGT CTGTCTCCGG TTCGAGCCGC
    CTCACCAGCC GGCAGCACTC TCTGGAGAGC CGGCAGTCCT TAGACCTGTC CGATAGCCCC
    ACTGGAAGTA GTCGATATGC TTCATCAGGG GAGATTAGTC GTGGCTCCTC ACAGCTCAGT
    GATGACTTTG ACCCAGATGA TGACAGCTTG CAAGGTTCAG AAATAGAGGA AGAAAGGGAT
    AGAGACTCTT ATCACTCCTG TCATAGTTCT GTCAGCTGCC GCAAGGACTC GCCAAATTGG
    GAAGGAGAGG AGGAGCGTGA AGTTTATAGT GAAGATGAGG AAGAATATCC TGACAGTAGG
    GGGCAGCTCT CTGACCAGGA GCTGTATGAT GATGACCAGC TTGAGGAAGA AGAGAAAAAA
    GGAAGATTAG CATATGGGCA GGAGGATTCC ATCTATCAGT CATTGGAGGC TGAGGAGAAG
    GAACCCCTGG ATATTCCATT GGAGAAGGAC AGGACACCAG AAGCTGTGCC AGAGGAGGTA
    GTAGAGCCAA CAGTACCAGC AGTCCAGTCT CCCCAAGCTG CAGACAGTTA CAGAGAAGAA
    GAACCTGGAA TGTCCAGGGC CAAATCTAAT TGGTTAAGAG CATTCAACAA GGTGCGCCTG
    CAGCTGCAAG AGGCACGAGG TGAAGGGACT GGGGAGATGT CCAAATCCCT CTGGTTTAAG
    GATGGGCCAG CTGGTGGTCT TATAATTATA GACAGTATGC CAGACATACG AAAGAGGAAA
    CCAATCCCGT TGGTCAGTGA CCTGGCAATG TCACTGGTTC AGTCACGGAA GGCTGGGATT
    ACTTCTGCTC TTGCAACAAG CACCTTAAAC AATGAAGAGC TAAAAAACCA TGTTTACAAA
    AAGACCCTGC AAGCTTTAAT ATACCCAATA TCCAGCACCA CTCCCCACAA CTTTGAAGTT
    TGGACTGCCA CAACTCCTAC CTATTGTTAT GAATGCGAAG GGCTTCTGTG GGGAATCGCT
    CGGCAGGGCA TGCGTTGCAC AGAGTGTGGT GTCAAGTGCC ATGAGAAGTG CCAGGATCTG
    CTGAATGCAG ATTGTCTTCA AAGAGCTGCA GAGAAGAGCT CAAAACATGG TGCAGAAGAC
    CGGACTCAGA ATATTATTAT GGTCTTGAAG GATCGTATGA AGATCCGGGA GAGGAACAAG
    CCAGAAATAT TTGAGCTGAT CCAGGAAGTA TTTGGGGTAT TAAAGGCTAC ACACGCTCAG
    CAGATAAAGG CTGTGAAACA GAGTGTGCTG GATGGTACTT CCAAATGGTC AGCAAAGATC
    AGCATAACTG TTGTATGTGC ACAGGGTCTA CAGGCCAAAG ATAAGACAGG CTCCAGTGAC
    CCATATGTTA CTGTTCAAGT TGGGAAAACC AAGAAAAGAA CCAAAACAAT CTATGGAAAT
    CTTAATCCTG TCTGGGAAGA GAATTTTCAC TTTGAATGCC ACAACTCCTC AGATCGTATT
    AAAGTACGCG TGTGGGATGA AGATGATGAT ATCAAATCTC GTGTGAAGCA GAGATTTAAA
    AGAGAATCAG ATGACTTCCT TGGACAAACA ATTATTGAAG TCAGAACACT TAGCGGAGAG
    ATGGATGTGT GGTATAACTT GGATAAGAGA ACAGATAAAT CCGCTGTCTC TGGGGCAATC
    CGTCTTCATA TAAGTGTGGA AATAAAAGGA GAGGAAAAGG TGGCTCCCTA TCACGTGCAA
    TACACATGTC TACATGAGAA CTTATTCCAT TACCTGACGG ATGTGCAGAA TAATGGTGTG
    GTCAAGATTC CAGATGCAAA AGGAGACGAC GCCTGGAAAG TTTATTTTGA TGAGACAGCC
    CAGGAAATTG TGGATGAATT TGCAATGAGA TATGGAGTGG AGTCCATATA CCAGGCCATG
    ACGCATTTTG CCTGCCTTTC ATCTAAATAT ATGTGTCCAG GAGTACCAGC TGTAATGAGT
    ACTCTTCTTG CCAACATTAA TGCTTACTAT GCGCACACAA CTGCATCTAC TAATGTGTCT
    GCCTCCGACC GATTCTCTGC CTCAAACTTT GGGAAGGAGA GATTTGTAAA GCTGCTTGAC
    CAGTTGCACA ATTCCTTACG CATTGACCTG TCCATGTATC GGAACAACTT CCCTGCTAGC
    AGCCCTGAAA GACTCCAAGA TCTCAAGTCC ACTGTAGATT TACTAACTAG CATAACATTT
    TTCCGGATGA AGGTACAAGA GCTTCAGAGC CCACCACGGG CAAGCCAAGT TGTTAAGGAC
    TGTGTGAAGG CATGCCTTAA CTCAACCTAC GAATATATCT TTAACAACTG CCATGAACTT
    TATAGCCGAG AATATCAAAG TGACCCTGCT AAAAAAGGGG AGGTACCCCC AGAAGAACAG
    GGGCCAAGTA TAAAGAACTT AGATTTTTGG TCCAAACTAA TCACATTGAT TGTGTCTATC
    ATAGAGGAAG ATAAGAACTC ATATACACCT TGTCTAAACC AGTTTCCTCA GGAGCTAAAT
    GTTGGTAAGA TTAGTGCTGA GGTCATGTGG AGTCTCTTTG CTCAAGACAT GAGATATGCT
    ATGGAAGAAC ACGACAAACA CCGCCTGTGT AAGAGTGCAG ACTACATGAA CTTGCATTTT
    AAGGTCAAGT GGTTGTACAA TGAATATGTA ACTGAGCTAC CATCTTTTAA GAGCAAGGTT
    CCAGAATATC CCGCCTGGTT TGAGCCTTTT GTAATTCAGT GGCTGGATGA AAATGAAGAG
    GTCTCCCGGG ATTTTCTGCA TGGAGCACTT GAAAGAGATA AGAAGGATGG GTTCCAGCAG
    ACCTCTGAAC ATGCCCTGTT CTCCTGCTCT GTGGTAGATG TTTTCTCACA ACTTAACCAG
    AGCTTTGAGA TCATTAAGAA GTTGGAGTGT CCAGACCCAC AAATTGTGGG GCATTACATG
    CGTAGATTTG CCAAGACGAT CAGTAATGTT CTGCTGCAGT ATGCTGAAAT TATTTCCAAA
    GATTTTGCAT CTCATTGCTC CAAAGAGAAG AAAGAAGAAA AAGTGCCCTG TATTCTTATG
    AACAATATCC AGCAACTTCG AGTGCAACTA GAAAAAATGT TTGAAGCAAT GGGAGGAAAG
    GAGCTGGATG CAGAAACAAG TGACATTCTT AAAGAGCTGC AGGTGAAACT CAACAATGTC
    CTAGATGAGC TAAGCAGAGT GTTTACCACC AGTTTTCAGG CACAAATTGA GGAGTGTGTG
    AAGCAGATGG GTGACATTCT GAGCCAGGTA AAAGGGACCG GAAATGTACC AGCCAGTAAC
    TGCAGCAGTG TGGCTCAGGA TGCAGATAAT GTATTGCAGC CTATCCTGGA TCCTCTGGAC
    AGCAACCTGA CACTATTTGC CAAGATCTGT GAAAAGACAG TCCTGAAGCG GGTACTAAAA
    GAACTCTGGA AGCTTGTGAT GAATACAATG GAGAAAACGA TTGTCCTTCC ACCACTAACA
    GACCAAACAG GAACCCACCT TATCATTAAT GCAGCCAAGG AACTGGGCCA GTTATCCAAA
    CTAAAGGATC ACATGGTGCG AGAGGAAGCC AAGAGCCTCA CCCCTAAGCA GTGTGCAGTG
    GTGGAGCTTG CACTTGATAC AATTAAGCAA TATTTCCATG CTGGAGGTGT TGGTCTCAAG
    AAGACATTCC TGGAGAAGAG TCCAGACCTA CAATCTCTAC GCTATGCACT TTCTCTTTAC
    ACACAAGCAA CCGATACGCT AATCAAAACC TTCGTCCAGA CACAGTCTGC TCAGGGATCT
    GGGGTGGATG ATTCTGTTGG GGAAGTTTCT ATCCATGTGG AGTTATTTAC TCACCCAGGA
    ACAGGCGAGC ACAAGATCAG CGTAAAGGTT GTAGCGGCCA ATGACCTTAA ATGGCAGACG
    TCAGGGATTT TCCGTCCCTT TATTGAGGTA AACATGATTG GTCCGAATCT GAGTGACAAA
    AAAAGGAAAT TTACCACCAA GTCCAAGAAC AACAGCTGGG CCCCAAAATA TAACGAGAGC
    TTCCAATTTA CCCTGGGTAA TGAGACTGGT CCAGAGAGTT ATGAGCTGCA AGTATGTGTG
    AAAGATTACT GTTTTGCTCG GGAAGACCGC ACAGTGGGAA TGGCTGTCAT GCAACTGCGA
    GATCTGGCAC AAAGGGGTAG CTGTGCCTGT TGGCTCCCTC TTGGCCGCCG CATTCATATG
    GATGAGACAG GACTGACCAT CTTGCGCATA CTATCACAAA GAAACAATGA TGAGGTGGCC
    AAAGAGTTTG TCAAACTCAA ATCTGACACC CGCTCTCCAG AAGAGGGGAG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017952307.1
    CDS188..5281
    Translation

    Target ORF information:

    RefSeq Version XM_018096818.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis unc-13 homolog A (unc13a), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018096818.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCTTTGC TTTGCGTGGG AGTAAAAAAG GCCACGTTTG ATGGAGCACA AGAGAAGTTT 
    AATGCCTACG TAACCTTGAA GGTGCAAAAT GTGAAGAGCA CAACGATAGC GGTGCGGGGA
    AACCAGCCCT GCTGGGAACA GGATTTCATG TTTGAGATTA ATCGTTTAGA CCTTGGTCTA
    ACAGTGGAGG TCTGGAACAA AGGATTGATC TGGGACACCA TGGTGGGAAC AGTATGGATT
    CCTTTAAGGA CAATCCGACA ATCCAATGAG GAGGGTCCTG GGGAATGGCT GACACTTGAT
    TCACAGGTCA TAATGACAGA TAATGAGATT TGTGGTACCA AAGATCCAAC CTTCCACCGC
    ATCTTACTGG ATACCCGATT TGAGCTTCCT CTAGATATAC CAGAAGAAGA AGCTCGATAC
    TGGGCAAAGA AACTCGAGCA ACTTAATGCA ATGAGGGACC AGGATGATTA TGTTTTTCAA
    GAAGAAATTC CGGAGAATCC TCTTCCTGTC CATGGATCTC AATGTTGTAA CTGGAATTAT
    TTCGGCTGGG GGGAGCAGCA CAATGAGGAT CCCGATAGTG CAGTGGATGA TCGGGATAGT
    GATTACCGCA GTGAAACCAG CAACAGTATC CCGCCCCCCT ACTACTCCAC CTCACAGCCT
    AATGCCTCTG TGCACCAGTA CCCTGTACGA CAGCAACACA GTTCTCATGG ATCTTACACT
    GATTCAGTGC ACAGCTATGA GATGGACTTC TCTGACCATC GTCCAATCAG GCGTTACGAT
    AGCGCAGACT CTGCCACTAA CGGGCGCAGT GATGTGGAGT CTGTCTCCGG TTCGAGCCGC
    CTCACCAGCC GGCAGCACTC TCTGGAGAGC CGGCAGTCCT TAGACCTGTC CGATAGCCCC
    ACTGGAAGTA GTCGATATGC TTCATCAGGG GAGATTAGTC GTGGCTCCTC ACAGCTCAGT
    GATGACTTTG ACCCAGATGA TGACAGCTTG CAAGGTTCAG AAATAGAGGA AGAAAGGGAT
    AGAGACTCTT ATCACTCCTG TCATAGTTCT GTCAGCTGCC GCAAGGACTC GCCAAATTGG
    GAAGGAGAGG AGGAGCGTGA AGTTTATAGT GAAGATGAGG AAGAATATCC TGACAGTAGG
    GGGCAGCTCT CTGACCAGGA GCTGTATGAT GATGACCAGC TTGAGGAAGA AGAGAAAAAA
    GGAAGATTAG CATATGGGCA GGAGGATTCC ATCTATCAGT CATTGGAGGC TGAGGAGAAG
    GAACCCCTGG ATATTCCATT GGAGAAGGAC AGGACACCAG AAGCTGTGCC AGAGGAGGTA
    GTAGAGCCAA CAGTACCAGC AGTCCAGTCT CCCCAAGCTG CAGACAGTTA CAGAGAAGAA
    GAACCTGGAA TGTCCAGGGC CAAATCTAAT TGGTTAAGAG CATTCAACAA GGTGCGCCTG
    CAGCTGCAAG AGGCACGAGG TGAAGGGACT GGGGAGATGT CCAAATCCCT CTGGTTTAAG
    GATGGGCCAG CTGGTGGTCT TATAATTATA GACAGTATGC CAGACATACG AAAGAGGAAA
    CCAATCCCGT TGGTCAGTGA CCTGGCAATG TCACTGGTTC AGTCACGGAA GGCTGGGATT
    ACTTCTGCTC TTGCAACAAG CACCTTAAAC AATGAAGAGC TAAAAAACCA TGTTTACAAA
    AAGACCCTGC AAGCTTTAAT ATACCCAATA TCCAGCACCA CTCCCCACAA CTTTGAAGTT
    TGGACTGCCA CAACTCCTAC CTATTGTTAT GAATGCGAAG GGCTTCTGTG GGGAATCGCT
    CGGCAGGGCA TGCGTTGCAC AGAGTGTGGT GTCAAGTGCC ATGAGAAGTG CCAGGATCTG
    CTGAATGCAG ATTGTCTTCA AAGAGCTGCA GAGAAGAGCT CAAAACATGG TGCAGAAGAC
    CGGACTCAGA ATATTATTAT GGTCTTGAAG GATCGTATGA AGATCCGGGA GAGGAACAAG
    CCAGAAATAT TTGAGCTGAT CCAGGAAGTA TTTGGGGTAT TAAAGGCTAC ACACGCTCAG
    CAGATAAAGG CTGTGAAACA GAGTGTGCTG GATGGTACTT CCAAATGGTC AGCAAAGATC
    AGCATAACTG TTGTATGTGC ACAGGGTCTA CAGGCCAAAG ATAAGACAGG CTCCAGTGAC
    CCATATGTTA CTGTTCAAGT TGGGAAAACC AAGAAAAGAA CCAAAACAAT CTATGGAAAT
    CTTAATCCTG TCTGGGAAGA GAATTTTCAC TTTGAATGCC ACAACTCCTC AGATCGTATT
    AAAGTACGCG TGTGGGATGA AGATGATGAT ATCAAATCTC GTGTGAAGCA GAGATTTAAA
    AGAGAATCAG ATGACTTCCT TGGACAAACA ATTATTGAAG TCAGAACACT TAGCGGAGAG
    ATGGATGTGT GGTATAACTT GGATAAGAGA ACAGATAAAT CCGCTGTCTC TGGGGCAATC
    CGTCTTCATA TAAGTGTGGA AATAAAAGGA GAGGAAAAGG TGGCTCCCTA TCACGTGCAA
    TACACATGTC TACATGAGAA CTTATTCCAT TACCTGACGG ATGTGCAGAA TAATGGTGTG
    GTCAAGATTC CAGATGCAAA AGGAGACGAC GCCTGGAAAG TTTATTTTGA TGAGACAGCC
    CAGGAAATTG TGGATGAATT TGCAATGAGA TATGGAGTGG AGTCCATATA CCAGGCCATG
    ACGCATTTTG CCTGCCTTTC ATCTAAATAT ATGTGTCCAG GAGTACCAGC TGTAATGAGT
    ACTCTTCTTG CCAACATTAA TGCTTACTAT GCGCACACAA CTGCATCTAC TAATGTGTCT
    GCCTCCGACC GATTCTCTGC CTCAAACTTT GGGAAGGAGA GATTTGTAAA GCTGCTTGAC
    CAGTTGCACA ATTCCTTACG CATTGACCTG TCCATGTATC GGAACAACTT CCCTGCTAGC
    AGCCCTGAAA GACTCCAAGA TCTCAAGTCC ACTGTAGATT TACTAACTAG CATAACATTT
    TTCCGGATGA AGGTACAAGA GCTTCAGAGC CCACCACGGG CAAGCCAAGT TGTTAAGGAC
    TGTGTGAAGG CATGCCTTAA CTCAACCTAC GAATATATCT TTAACAACTG CCATGAACTT
    TATAGCCGAG AATATCAAAG TGACCCTGCT AAAAAAGGGG AGGTACCCCC AGAAGAACAG
    GGGCCAAGTA TAAAGAACTT AGATTTTTGG TCCAAACTAA TCACATTGAT TGTGTCTATC
    ATAGAGGAAG ATAAGAACTC ATATACACCT TGTCTAAACC AGTTTCCTCA GGAGCTAAAT
    GTTGGTAAGA TTAGTGCTGA GGTCATGTGG AGTCTCTTTG CTCAAGACAT GAGATATGCT
    ATGGAAGAAC ACGACAAACA CCGCCTGTGT AAGAGTGCAG ACTACATGAA CTTGCATTTT
    AAGGTCAAGT GGTTGTACAA TGAATATGTA ACTGAGCTAC CATCTTTTAA GAGCAAGGTT
    CCAGAATATC CCGCCTGGTT TGAGCCTTTT GTAATTCAGT GGCTGGATGA AAATGAAGAG
    GTCTCCCGGG ATTTTCTGCA TGGAGCACTT GAAAGAGATA AGAAGGATGG GTTCCAGCAG
    ACCTCTGAAC ATGCCCTGTT CTCCTGCTCT GTGGTAGATG TTTTCTCACA ACTTAACCAG
    AGCTTTGAGA TCATTAAGAA GTTGGAGTGT CCAGACCCAC AAATTGTGGG GCATTACATG
    CGTAGATTTG CCAAGACGAT CAGTAATGTT CTGCTGCAGT ATGCTGAAAT TATTTCCAAA
    GATTTTGCAT CTCATTGCTC CAAAGAGAAG AAAGAAGAAA AAGTGCCCTG TATTCTTATG
    AACAATATCC AGCAACTTCG AGTGCAACTA GAAAAAATGT TTGAAGCAAT GGGAGGAAAG
    GAGCTGGATG CAGAAACAAG TGACATTCTT AAAGAGCTGC AGGTGAAACT CAACAATGTC
    CTAGATGAGC TAAGCAGAGT GTTTACCACC AGTTTTCAGG CACAAATTGA GGAGTGTGTG
    AAGCAGATGG GTGACATTCT GAGCCAGGTA AAAGGGACCG GAAATGTACC AGCCAGTAAC
    TGCAGCAGTG TGGCTCAGGA TGCAGATAAT GTATTGCAGC CTATCCTGGA TCCTCTGGAC
    AGCAACCTGA CACTATTTGC CAAGATCTGT GAAAAGACAG TCCTGAAGCG GGTACTAAAA
    GAACTCTGGA AGCTTGTGAT GAATACAATG GAGAAAACGA TTGTCCTTCC ACCACTAACA
    GACCAAACAG GAACCCACCT TATCATTAAT GCAGCCAAGG AACTGGGCCA GTTATCCAAA
    CTAAAGGATC ACATGGTGCG AGAGGAAGCC AAGAGCCTCA CCCCTAAGCA GTGTGCAGTG
    GTGGAGCTTG CACTTGATAC AATTAAGCAA TATTTCCATG CTGGAGGTGT TGGTCTCAAG
    AAGACATTCC TGGAGAAGAG TCCAGACCTA CAATCTCTAC GCTATGCACT TTCTCTTTAC
    ACACAAGCAA CCGATACGCT AATCAAAACC TTCGTCCAGA CACAGTCTGC TCAGGGATCT
    GGGGTGGATG ATTCTGTTGG GGAAGTTTCT ATCCATGTGG AGTTATTTAC TCACCCAGGA
    ACAGGCGAGC ACAAGATCAG CGTAAAGGTT GTAGCGGCCA ATGACCTTAA ATGGCAGACG
    TCAGGGATTT TCCGTCCCTT TATTGAGGTA AACATGATTG GTCCGAATCT GAGTGACAAA
    AAAAGGAAAT TTACCACCAA GTCCAAGAAC AACAGCTGGG CCCCAAAATA TAACGAGAGC
    TTCCAATTTA CCCTGGGTAA TGAGACTGGT CCAGAGAGTT ATGAGCTGCA AGTATGTGTG
    AAAGATTACT GTTTTGCTCG GGAAGACCGC ACAGTGGGAA TGGCTGTCAT GCAACTGCGA
    GATCTGGCAC AAAGGGGTAG CTGTGCCTGT TGGCTCCCTC TTGGCCGCCG CATTCATATG
    GATGAGACAG GACTGACCAT CTTGCGCATA CTATCACAAA GAAACAATGA TGAGGTGGCC
    AAAGAGTTTG TCAAACTCAA ATCTGACACC CGCTCTCCAG AAGAGGGGAG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa48429
    Clone ID Related Accession (Same CDS sequence) XM_031904660.1
    Accession Version XM_031904660.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5229bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein unc-13 homolog A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGTCTTTGC TTTGCGTGGG AGTAAAAAAG GCCACGTTTG ATGGAGCACA AGAGAAGTTT 
    AATGCCTACG TAACCTTGAA GGTGCAAAAT GTGAAGAGCA CAACGATAGC GGTGCGGGGA
    AACCAGCCCT GCTGGGAACA GGATTTCATG TTTGAGATTA ATCGTTTAGA CCTTGGTCTA
    ACAGTGGAGG TCTGGAACAA AGGATTGATC TGGGACACCA TGGTGGGAAC AGTATGGATT
    CCTTTAAGGA CAATCCGACA ATCCAATGAG GAGGGTCCTG GGGAATGGCT GACACTTGAT
    TCACAGGTCA TAATGACAGA TAATGAGATT TGTGGTACCA AAGATCCAAC CTTCCACCGC
    ATCTTACTGG ATACCCGATT TGAACTTCCT CTAGATATAC CAGAAGAAGA AGCTCGATAC
    TGGGCAAAGA AACTTGAGCA ACTTAATGCA ATGAGGGACC AGGATGATTA TGTTTTTCAA
    GAAGAAATTC CGGAGAATCC TCTTCCCGTC CATGGATCTC AATGTTGTAA CTGGAATTAT
    TTCGGCTGGG GGGAGCAGCA CAATGAGGAT CCCGATAGTG CAGTGGATGA TCGGGATAGT
    GATTACCGCA GTGAAACCAG CAACAGTATC CCGCCCCCCT ACTACTCCAC CTCACAGCCT
    AATGCCTCTG TGCACCAGTA CCCTGTACGA CAGCAACACA GTTCTCATGG ATCTTACACT
    GATTCAGTGC ACAGCTATGA GATGGACTTC TCTGACCATC GTCCAATCAG CAGGCGTTAC
    GATAGCGCAG ACTCTGCCAC TAACGGGCGC AGTGATGTGG AGTCTGTCTC CGGTTCGAGC
    CGCCTCACCA GCCGGCAGCA CTCTCTGGAG AGCCGGCAGT CCTTAGACCT GTCCGATAGC
    CCCACTGGAA GTAGTCGATA TGCTTCATCA GGGGAGATTA GTCGTGGCTC CTCACAGCTC
    AGTGATGACT TTGACCCAGA TGATGACAGC TTGCAAGGTT CAGAAATAGA GGAAGAAAGG
    GATAGAGACT CTTATCACTC CTGTCATAGT TCTGTCAGCT GCCGCAAGGA CTCGCCAAAT
    TGGGAAGGAG AGGAGGAGCC TGAAGTTTAT AGTGAAGATG AGGAAGAATA TCCTGACAGT
    AGGGGGCAGC TCTCTGACCA GGAGCTGTAT GATGATGACC AGCTTGAGGA AGAAGAGAAA
    AAAGGAAGAT TAGCATATGG GCAGGAGGAT TCCATCTATC AGTCATTGGA GGCTGAGGAG
    AAGGAACCCC TGGATATTCC ATTGGAGAAG GACAGGACAC CAGAAGCTGT GCCAGAGGAG
    GTAGTAGAGC CAACAGTACC AGCAGTCCAG TCTCCCCAAG CTGCAGACAG TTACAGAGAA
    GAAGAACCTG GAATGTCCAG GGCCAAATCT AATTGGTTAA GAGCATTCAA CAAGGTGCGC
    CTGCAGCTGC AAGAGGCACG AGGTGAAGGG ACTGGGGAGA TGTCCAAATC CCTCTGGTTT
    AAGGATGGGC CAGCTGGTGG TCTTATAATT ATAGACAGTA TGCCAGACAT ACGAAAGAGG
    AAACCAATCC CGTTGGTCAG TGACCTGGCA ATGTCACTGG TTCAGTCACG GAAGGCTGGG
    ATTACTTCTG CTCTTGCAAC AAGCACCTTA AACAATGAAG AGCTAAAAAA CCATGTTTAC
    AAAAAGACCC TGCAAGCTTT AATATACCCA ATATCCAGCA CCACTCCCCA CAACTTTGAA
    GTTTGGACTG CCACAACTCC TACCTATTGT TATGAATGCG AAGGGCTTCT GTGGGGAATC
    GCTCGGCAGG GCATGCGTTG CACAGAGTGT GGTGTCAAGT GCCATGAGAA GTGCCAGGAT
    CTGCTGAATG CAGATTGTCT TCAAAGAGCT GCAGAGAAGA GCTCAAAACA TGGTGCAGAA
    GACCGGACTC AGAATATTAT TATGGTCTTG AAGGATCGTA TGAAGATCCG GGAGAGGAAC
    AAGCCAGAAA TATTTGAGCT GATCCAGGAA GTATTTGGGG TATTAAAGGC TACACACGCT
    CAGCAGATAA AGGCTGTGAA ACAGAGTGTG CTGGATGGTA CTTCCAAATG GTCAGCAAAG
    ATCAGCATAA CTGTTGTATG TGCACAGGGT CTACAGGCCA AAGATAAGAC AGGCTCCAGT
    GACCCATATG TTACTGTTCA AGTTGGGAAA ACCAAGAAAA GAACCAAAAC AATCTATGGA
    AATCTTAATC CTGTCTGGGA AGAGAATTTT CACTTTGAAT GCCACAACTC CTCAGATCGT
    ATTAAAGTAC GCGTGTGGGA TGAAGATGAT GATATCAAAT CTCGTGTGAA GCAGAGATTT
    AAAAGAGAAT CAGATGACTT CCTTGGACAA ACAATTATTG AAGTCAGAAC ACTTAGCGGA
    GAGATGGATG TGTGGTATAA CTTGGATAAG AGAACAGATA AATCCGCTGT CTCTGGGGCA
    ATCCGTCTTC ATATAAGTGT GGAAATAAAA GGAGAGGAAA AGGTGGCTCC CTATCACGTG
    CAATACACAT GTCTACATGA GAACTTATTC CATTACCTGA CGGATGTGCA GAATAATGGT
    GTGGTCAAGA TTCCAGATGC AAAAGGAGAC GACGCCTGGA AAGTTTATTT TGATGAGACA
    GCCCAGGAAA TTGTGGATGA ATTTGCAATG AGATATGGAG TGGAGTCCAT ATATCAGGCC
    ATGACGCATT TTGCCTGCCT TTCATCTAAA TATATGTGTC CAGGAGTACC AGCTGTAATG
    AGTACTCTTC TTGCCAACAT TAATGCTTAC TATGCGCACA CAACTGCATC CACTAATGTG
    TCTGCCTCCG ACCGATTCTC TGCCTCAAAC TTTGGGAAGG AGAGATTTGT AAAGCTGCTT
    GACCAGTTGC ACAATTCCTT ACGCATTGAC CTGTCCATGT ATCGGAACAA CTTCCCTGCT
    AGCAGCCCTG AAAGACTCCA AGATCTCAAG TCCACTGTAG ATTTACTAAC TAGCATAACA
    TTTTTCCGGA TGAAGGTACA AGAGCTTCAG AGCCCACCAC GGGCAAGCCA AGTTGTTAAG
    GACTGTGTGA AGGCATGCCT TAACTCAACC TACGAATATA TCTTTAACAA CTGCCATGAA
    CTTTATAGCC GAGAATATCA AAGTGACCCT GCTAAAAAAG GGGAGGTACC CCCAGAAGAA
    CAGGGGCCAA GTATAAAGAA CTTAGATTTT TGGTCCAAAC TAATCACATT GATTGTGTCT
    ATCATAGAGG AAGATAAGAA CTCATATACA CCTTGTCTAA ACCAGTTTCC TCAGGAGCTA
    AATGTTGGTA AGATTAGTGC TGAGGTCATG TGGAGTCTCT TTGCTCAAGA CATGAGATAT
    GCTATGGAAG AACACGACAA ACACCGCCTG TGTAAGAGTG CAGACTACAT GAACTTGCAT
    TTTAAGGTCA AGTGGTTGTA CAATGAATAT GTAACTGAGC TACCATCTTT TAAGAGCAAG
    GTTCCAGAAT ATCCCGCCTG GTTTGAGCCT TTTGTAATTC AGTGGCTGGA TGAAAATGAA
    GAGGTCTCCC GGGATTTTCT GCATGGAGCA CTTGAAAGAG ATAAGAAGGA TGGGTTCCAG
    CAGACCTCTG AACATGCCCT GTTCTCCTGC TCTGTGGTGG ATGTTTTCTC ACAACTTAAC
    CAGAGCTTTG AGATCATTAA GAAGTTGGAG TGTCCAGACC CACAAATTGT GGGGCATTAC
    ATGCGTAGAT TTGCCAAGAC GATCAGTAAT GTTCTGCTGC AGTATGCTGA AATTATTTCC
    AAAGATTTTG CATCTCATTG CTCCAAAGAG AAGAAAGAAG AAAAAGTGCC CTGTATTCTT
    ATGAACAACA TCCAGCAACT TCGAGTGCAA CTAGAAAAAA TGTTTGAAGC AATGGGAGGA
    AAGGAGCTGG ATGCAGAAAC AAGTGACATT CTTAAAGAGC TGCAGGTGAA ACTCAACAAT
    GTCCTAGATG AGCTAAGCAG AGTGTTTACC ACCAGTTTTC AGGCACAAAT TGAGGAGTGT
    GTGAAGCAGA TGGGTGACAT CCTGAGCCAG GTAAAAGGGA CCGGAAATGT ACCAGCCAGT
    AACTGCAGCA GTGTGGCTCA GGATGCAGAT AATGTATTGC AGCCTATCCT GGATCCTCTG
    GACAGCAACC TGACACTATT TGCCAAGATC TGTGAAAAGA CAGTCCTGAA GCGGGTACTA
    AAAGAACTCT GGAAGCTTGT GATGAATACA ATGGAGAAAA CGATTGTCCT TCCACCACTA
    ACAGACCAAA CAATGATAGG GACGCTGCTG AGAAAACATG GCAAGGGAAT AGAGAAGAGC
    AGGGTGAAGC TGCCGTACCA CGCAGATGGA ACCCACCTTA TCATTAATGC AGCCAAGGAA
    CTGGGCCAGT TATCCAAACT AAAGGATCAC ATGGTGCGAG AGGAAGCCAA GAGCCTCACC
    CCTAAGCAGT GTGCAGTGGT GGAGCTTGCA CTTGATACAA TTAAGCAATA TTTCCATGCT
    GGAGGTGTTG GTCTCAAGAA GACATTCCTG GAGAAGAGTC CAGACCTACA ATCTCTACGC
    TATGCACTTT CTCTTTACAC ACAAGCAACC GATACGCTAA TCAAAACCTT CGTCCAGACA
    CAGTCTGCTC AGGTTCATGG TGGTAAAGGT ATTAGGTTTA CACTTAATGA AGAGACTTAT
    CCAGAAAAGG GATCTGGGGT GGATGATTCT GTTGGGGAAG TTTCTATCCA TGTGGAGTTA
    TTTACTCACC CAGGAACAGG CGAGCACAAG ATCAGCGTAA AGGTTGTAGC GGCCAATGAC
    CTTAAATGGC AGACGTCAGG GATTTTCCGT CCCTTTATTG AGGTAAACAT GATTGGTCCG
    AATCTGAGTG ACAAAAAAAG GAAATTTACC ACCAAGTCCA AGAACAACAG CTGGGCCCCA
    AAATATAACG AGAGCTTCCA ATTTACCCTG GGTAATGAGA CTGGTCCAGA GAGTTATGAG
    CTGCAAGTAT GTGTGAAAGA TTACTGTTTT GCTCGGGAAG ACCGCACAGT GGGAATGGCT
    GTCATGCAAC TGCGAGATCT GGCACAAAGG GGTAGCTGTG CCTGTTGGCT CCCTCTTGGC
    CGCCGCATTC ATATGGATGA GACAGGACTG ACCATCTTGC GCATACTATC ACAAAGAAAC
    AATGATGAGG TGGCCAAAGA GTTTGTCAAA CTCAAATCTG ACACCCGCTC TCCAGAAGAG
    GGGAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031760520.1
    CDS188..5416
    Translation

    Target ORF information:

    RefSeq Version XM_031904660.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis unc-13 homolog A (unc13a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031904660.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGTCTTTGC TTTGCGTGGG AGTAAAAAAG GCCACGTTTG ATGGAGCACA AGAGAAGTTT 
    AATGCCTACG TAACCTTGAA GGTGCAAAAT GTGAAGAGCA CAACGATAGC GGTGCGGGGA
    AACCAGCCCT GCTGGGAACA GGATTTCATG TTTGAGATTA ATCGTTTAGA CCTTGGTCTA
    ACAGTGGAGG TCTGGAACAA AGGATTGATC TGGGACACCA TGGTGGGAAC AGTATGGATT
    CCTTTAAGGA CAATCCGACA ATCCAATGAG GAGGGTCCTG GGGAATGGCT GACACTTGAT
    TCACAGGTCA TAATGACAGA TAATGAGATT TGTGGTACCA AAGATCCAAC CTTCCACCGC
    ATCTTACTGG ATACCCGATT TGAACTTCCT CTAGATATAC CAGAAGAAGA AGCTCGATAC
    TGGGCAAAGA AACTTGAGCA ACTTAATGCA ATGAGGGACC AGGATGATTA TGTTTTTCAA
    GAAGAAATTC CGGAGAATCC TCTTCCCGTC CATGGATCTC AATGTTGTAA CTGGAATTAT
    TTCGGCTGGG GGGAGCAGCA CAATGAGGAT CCCGATAGTG CAGTGGATGA TCGGGATAGT
    GATTACCGCA GTGAAACCAG CAACAGTATC CCGCCCCCCT ACTACTCCAC CTCACAGCCT
    AATGCCTCTG TGCACCAGTA CCCTGTACGA CAGCAACACA GTTCTCATGG ATCTTACACT
    GATTCAGTGC ACAGCTATGA GATGGACTTC TCTGACCATC GTCCAATCAG CAGGCGTTAC
    GATAGCGCAG ACTCTGCCAC TAACGGGCGC AGTGATGTGG AGTCTGTCTC CGGTTCGAGC
    CGCCTCACCA GCCGGCAGCA CTCTCTGGAG AGCCGGCAGT CCTTAGACCT GTCCGATAGC
    CCCACTGGAA GTAGTCGATA TGCTTCATCA GGGGAGATTA GTCGTGGCTC CTCACAGCTC
    AGTGATGACT TTGACCCAGA TGATGACAGC TTGCAAGGTT CAGAAATAGA GGAAGAAAGG
    GATAGAGACT CTTATCACTC CTGTCATAGT TCTGTCAGCT GCCGCAAGGA CTCGCCAAAT
    TGGGAAGGAG AGGAGGAGCC TGAAGTTTAT AGTGAAGATG AGGAAGAATA TCCTGACAGT
    AGGGGGCAGC TCTCTGACCA GGAGCTGTAT GATGATGACC AGCTTGAGGA AGAAGAGAAA
    AAAGGAAGAT TAGCATATGG GCAGGAGGAT TCCATCTATC AGTCATTGGA GGCTGAGGAG
    AAGGAACCCC TGGATATTCC ATTGGAGAAG GACAGGACAC CAGAAGCTGT GCCAGAGGAG
    GTAGTAGAGC CAACAGTACC AGCAGTCCAG TCTCCCCAAG CTGCAGACAG TTACAGAGAA
    GAAGAACCTG GAATGTCCAG GGCCAAATCT AATTGGTTAA GAGCATTCAA CAAGGTGCGC
    CTGCAGCTGC AAGAGGCACG AGGTGAAGGG ACTGGGGAGA TGTCCAAATC CCTCTGGTTT
    AAGGATGGGC CAGCTGGTGG TCTTATAATT ATAGACAGTA TGCCAGACAT ACGAAAGAGG
    AAACCAATCC CGTTGGTCAG TGACCTGGCA ATGTCACTGG TTCAGTCACG GAAGGCTGGG
    ATTACTTCTG CTCTTGCAAC AAGCACCTTA AACAATGAAG AGCTAAAAAA CCATGTTTAC
    AAAAAGACCC TGCAAGCTTT AATATACCCA ATATCCAGCA CCACTCCCCA CAACTTTGAA
    GTTTGGACTG CCACAACTCC TACCTATTGT TATGAATGCG AAGGGCTTCT GTGGGGAATC
    GCTCGGCAGG GCATGCGTTG CACAGAGTGT GGTGTCAAGT GCCATGAGAA GTGCCAGGAT
    CTGCTGAATG CAGATTGTCT TCAAAGAGCT GCAGAGAAGA GCTCAAAACA TGGTGCAGAA
    GACCGGACTC AGAATATTAT TATGGTCTTG AAGGATCGTA TGAAGATCCG GGAGAGGAAC
    AAGCCAGAAA TATTTGAGCT GATCCAGGAA GTATTTGGGG TATTAAAGGC TACACACGCT
    CAGCAGATAA AGGCTGTGAA ACAGAGTGTG CTGGATGGTA CTTCCAAATG GTCAGCAAAG
    ATCAGCATAA CTGTTGTATG TGCACAGGGT CTACAGGCCA AAGATAAGAC AGGCTCCAGT
    GACCCATATG TTACTGTTCA AGTTGGGAAA ACCAAGAAAA GAACCAAAAC AATCTATGGA
    AATCTTAATC CTGTCTGGGA AGAGAATTTT CACTTTGAAT GCCACAACTC CTCAGATCGT
    ATTAAAGTAC GCGTGTGGGA TGAAGATGAT GATATCAAAT CTCGTGTGAA GCAGAGATTT
    AAAAGAGAAT CAGATGACTT CCTTGGACAA ACAATTATTG AAGTCAGAAC ACTTAGCGGA
    GAGATGGATG TGTGGTATAA CTTGGATAAG AGAACAGATA AATCCGCTGT CTCTGGGGCA
    ATCCGTCTTC ATATAAGTGT GGAAATAAAA GGAGAGGAAA AGGTGGCTCC CTATCACGTG
    CAATACACAT GTCTACATGA GAACTTATTC CATTACCTGA CGGATGTGCA GAATAATGGT
    GTGGTCAAGA TTCCAGATGC AAAAGGAGAC GACGCCTGGA AAGTTTATTT TGATGAGACA
    GCCCAGGAAA TTGTGGATGA ATTTGCAATG AGATATGGAG TGGAGTCCAT ATATCAGGCC
    ATGACGCATT TTGCCTGCCT TTCATCTAAA TATATGTGTC CAGGAGTACC AGCTGTAATG
    AGTACTCTTC TTGCCAACAT TAATGCTTAC TATGCGCACA CAACTGCATC CACTAATGTG
    TCTGCCTCCG ACCGATTCTC TGCCTCAAAC TTTGGGAAGG AGAGATTTGT AAAGCTGCTT
    GACCAGTTGC ACAATTCCTT ACGCATTGAC CTGTCCATGT ATCGGAACAA CTTCCCTGCT
    AGCAGCCCTG AAAGACTCCA AGATCTCAAG TCCACTGTAG ATTTACTAAC TAGCATAACA
    TTTTTCCGGA TGAAGGTACA AGAGCTTCAG AGCCCACCAC GGGCAAGCCA AGTTGTTAAG
    GACTGTGTGA AGGCATGCCT TAACTCAACC TACGAATATA TCTTTAACAA CTGCCATGAA
    CTTTATAGCC GAGAATATCA AAGTGACCCT GCTAAAAAAG GGGAGGTACC CCCAGAAGAA
    CAGGGGCCAA GTATAAAGAA CTTAGATTTT TGGTCCAAAC TAATCACATT GATTGTGTCT
    ATCATAGAGG AAGATAAGAA CTCATATACA CCTTGTCTAA ACCAGTTTCC TCAGGAGCTA
    AATGTTGGTA AGATTAGTGC TGAGGTCATG TGGAGTCTCT TTGCTCAAGA CATGAGATAT
    GCTATGGAAG AACACGACAA ACACCGCCTG TGTAAGAGTG CAGACTACAT GAACTTGCAT
    TTTAAGGTCA AGTGGTTGTA CAATGAATAT GTAACTGAGC TACCATCTTT TAAGAGCAAG
    GTTCCAGAAT ATCCCGCCTG GTTTGAGCCT TTTGTAATTC AGTGGCTGGA TGAAAATGAA
    GAGGTCTCCC GGGATTTTCT GCATGGAGCA CTTGAAAGAG ATAAGAAGGA TGGGTTCCAG
    CAGACCTCTG AACATGCCCT GTTCTCCTGC TCTGTGGTGG ATGTTTTCTC ACAACTTAAC
    CAGAGCTTTG AGATCATTAA GAAGTTGGAG TGTCCAGACC CACAAATTGT GGGGCATTAC
    ATGCGTAGAT TTGCCAAGAC GATCAGTAAT GTTCTGCTGC AGTATGCTGA AATTATTTCC
    AAAGATTTTG CATCTCATTG CTCCAAAGAG AAGAAAGAAG AAAAAGTGCC CTGTATTCTT
    ATGAACAACA TCCAGCAACT TCGAGTGCAA CTAGAAAAAA TGTTTGAAGC AATGGGAGGA
    AAGGAGCTGG ATGCAGAAAC AAGTGACATT CTTAAAGAGC TGCAGGTGAA ACTCAACAAT
    GTCCTAGATG AGCTAAGCAG AGTGTTTACC ACCAGTTTTC AGGCACAAAT TGAGGAGTGT
    GTGAAGCAGA TGGGTGACAT CCTGAGCCAG GTAAAAGGGA CCGGAAATGT ACCAGCCAGT
    AACTGCAGCA GTGTGGCTCA GGATGCAGAT AATGTATTGC AGCCTATCCT GGATCCTCTG
    GACAGCAACC TGACACTATT TGCCAAGATC TGTGAAAAGA CAGTCCTGAA GCGGGTACTA
    AAAGAACTCT GGAAGCTTGT GATGAATACA ATGGAGAAAA CGATTGTCCT TCCACCACTA
    ACAGACCAAA CAATGATAGG GACGCTGCTG AGAAAACATG GCAAGGGAAT AGAGAAGAGC
    AGGGTGAAGC TGCCGTACCA CGCAGATGGA ACCCACCTTA TCATTAATGC AGCCAAGGAA
    CTGGGCCAGT TATCCAAACT AAAGGATCAC ATGGTGCGAG AGGAAGCCAA GAGCCTCACC
    CCTAAGCAGT GTGCAGTGGT GGAGCTTGCA CTTGATACAA TTAAGCAATA TTTCCATGCT
    GGAGGTGTTG GTCTCAAGAA GACATTCCTG GAGAAGAGTC CAGACCTACA ATCTCTACGC
    TATGCACTTT CTCTTTACAC ACAAGCAACC GATACGCTAA TCAAAACCTT CGTCCAGACA
    CAGTCTGCTC AGGTTCATGG TGGTAAAGGT ATTAGGTTTA CACTTAATGA AGAGACTTAT
    CCAGAAAAGG GATCTGGGGT GGATGATTCT GTTGGGGAAG TTTCTATCCA TGTGGAGTTA
    TTTACTCACC CAGGAACAGG CGAGCACAAG ATCAGCGTAA AGGTTGTAGC GGCCAATGAC
    CTTAAATGGC AGACGTCAGG GATTTTCCGT CCCTTTATTG AGGTAAACAT GATTGGTCCG
    AATCTGAGTG ACAAAAAAAG GAAATTTACC ACCAAGTCCA AGAACAACAG CTGGGCCCCA
    AAATATAACG AGAGCTTCCA ATTTACCCTG GGTAATGAGA CTGGTCCAGA GAGTTATGAG
    CTGCAAGTAT GTGTGAAAGA TTACTGTTTT GCTCGGGAAG ACCGCACAGT GGGAATGGCT
    GTCATGCAAC TGCGAGATCT GGCACAAAGG GGTAGCTGTG CCTGTTGGCT CCCTCTTGGC
    CGCCGCATTC ATATGGATGA GACAGGACTG ACCATCTTGC GCATACTATC ACAAAGAAAC
    AATGATGAGG TGGCCAAAGA GTTTGTCAAA CTCAAATCTG ACACCCGCTC TCCAGAAGAG
    GGGAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa48430
    Clone ID Related Accession (Same CDS sequence) XM_012954733.3
    Accession Version XM_012954733.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5226bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein unc-13 homolog A isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012954733.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGTCTTTGC TTTGCGTGGG AGTAAAAAAG GCCACGTTTG ATGGAGCACA AGAGAAGTTT 
    AATGCCTACG TAACCTTGAA GGTGCAAAAT GTGAAGAGCA CAACGATAGC GGTGCGGGGA
    AACCAGCCCT GCTGGGAACA GGATTTCATG TTTGAGATTA ATCGTTTAGA CCTTGGTCTA
    ACAGTGGAGG TCTGGAACAA AGGATTGATC TGGGACACCA TGGTGGGAAC AGTATGGATT
    CCTTTAAGGA CAATCCGACA ATCCAATGAG GAGGGTCCTG GGGAATGGCT GACACTTGAT
    TCACAGGTCA TAATGACAGA TAATGAGATT TGTGGTACCA AAGATCCAAC CTTCCACCGC
    ATCTTACTGG ATACCCGATT TGAACTTCCT CTAGATATAC CAGAAGAAGA AGCTCGATAC
    TGGGCAAAGA AACTTGAGCA ACTTAATGCA ATGAGGGACC AGGATGATTA TGTTTTTCAA
    GAAGAAATTC CGGAGAATCC TCTTCCCGTC CATGGATCTC AATGTTGTAA CTGGAATTAT
    TTCGGCTGGG GGGAGCAGCA CAATGAGGAT CCCGATAGTG CAGTGGATGA TCGGGATAGT
    GATTACCGCA GTGAAACCAG CAACAGTATC CCGCCCCCCT ACTACTCCAC CTCACAGCCT
    AATGCCTCTG TGCACCAGTA CCCTGTACGA CAGCAACACA GTTCTCATGG ATCTTACACT
    GATTCAGTGC ACAGCTATGA GATGGACTTC TCTGACCATC GTCCAATCAG GCGTTACGAT
    AGCGCAGACT CTGCCACTAA CGGGCGCAGT GATGTGGAGT CTGTCTCCGG TTCGAGCCGC
    CTCACCAGCC GGCAGCACTC TCTGGAGAGC CGGCAGTCCT TAGACCTGTC CGATAGCCCC
    ACTGGAAGTA GTCGATATGC TTCATCAGGG GAGATTAGTC GTGGCTCCTC ACAGCTCAGT
    GATGACTTTG ACCCAGATGA TGACAGCTTG CAAGGTTCAG AAATAGAGGA AGAAAGGGAT
    AGAGACTCTT ATCACTCCTG TCATAGTTCT GTCAGCTGCC GCAAGGACTC GCCAAATTGG
    GAAGGAGAGG AGGAGCCTGA AGTTTATAGT GAAGATGAGG AAGAATATCC TGACAGTAGG
    GGGCAGCTCT CTGACCAGGA GCTGTATGAT GATGACCAGC TTGAGGAAGA AGAGAAAAAA
    GGAAGATTAG CATATGGGCA GGAGGATTCC ATCTATCAGT CATTGGAGGC TGAGGAGAAG
    GAACCCCTGG ATATTCCATT GGAGAAGGAC AGGACACCAG AAGCTGTGCC AGAGGAGGTA
    GTAGAGCCAA CAGTACCAGC AGTCCAGTCT CCCCAAGCTG CAGACAGTTA CAGAGAAGAA
    GAACCTGGAA TGTCCAGGGC CAAATCTAAT TGGTTAAGAG CATTCAACAA GGTGCGCCTG
    CAGCTGCAAG AGGCACGAGG TGAAGGGACT GGGGAGATGT CCAAATCCCT CTGGTTTAAG
    GATGGGCCAG CTGGTGGTCT TATAATTATA GACAGTATGC CAGACATACG AAAGAGGAAA
    CCAATCCCGT TGGTCAGTGA CCTGGCAATG TCACTGGTTC AGTCACGGAA GGCTGGGATT
    ACTTCTGCTC TTGCAACAAG CACCTTAAAC AATGAAGAGC TAAAAAACCA TGTTTACAAA
    AAGACCCTGC AAGCTTTAAT ATACCCAATA TCCAGCACCA CTCCCCACAA CTTTGAAGTT
    TGGACTGCCA CAACTCCTAC CTATTGTTAT GAATGCGAAG GGCTTCTGTG GGGAATCGCT
    CGGCAGGGCA TGCGTTGCAC AGAGTGTGGT GTCAAGTGCC ATGAGAAGTG CCAGGATCTG
    CTGAATGCAG ATTGTCTTCA AAGAGCTGCA GAGAAGAGCT CAAAACATGG TGCAGAAGAC
    CGGACTCAGA ATATTATTAT GGTCTTGAAG GATCGTATGA AGATCCGGGA GAGGAACAAG
    CCAGAAATAT TTGAGCTGAT CCAGGAAGTA TTTGGGGTAT TAAAGGCTAC ACACGCTCAG
    CAGATAAAGG CTGTGAAACA GAGTGTGCTG GATGGTACTT CCAAATGGTC AGCAAAGATC
    AGCATAACTG TTGTATGTGC ACAGGGTCTA CAGGCCAAAG ATAAGACAGG CTCCAGTGAC
    CCATATGTTA CTGTTCAAGT TGGGAAAACC AAGAAAAGAA CCAAAACAAT CTATGGAAAT
    CTTAATCCTG TCTGGGAAGA GAATTTTCAC TTTGAATGCC ACAACTCCTC AGATCGTATT
    AAAGTACGCG TGTGGGATGA AGATGATGAT ATCAAATCTC GTGTGAAGCA GAGATTTAAA
    AGAGAATCAG ATGACTTCCT TGGACAAACA ATTATTGAAG TCAGAACACT TAGCGGAGAG
    ATGGATGTGT GGTATAACTT GGATAAGAGA ACAGATAAAT CCGCTGTCTC TGGGGCAATC
    CGTCTTCATA TAAGTGTGGA AATAAAAGGA GAGGAAAAGG TGGCTCCCTA TCACGTGCAA
    TACACATGTC TACATGAGAA CTTATTCCAT TACCTGACGG ATGTGCAGAA TAATGGTGTG
    GTCAAGATTC CAGATGCAAA AGGAGACGAC GCCTGGAAAG TTTATTTTGA TGAGACAGCC
    CAGGAAATTG TGGATGAATT TGCAATGAGA TATGGAGTGG AGTCCATATA TCAGGCCATG
    ACGCATTTTG CCTGCCTTTC ATCTAAATAT ATGTGTCCAG GAGTACCAGC TGTAATGAGT
    ACTCTTCTTG CCAACATTAA TGCTTACTAT GCGCACACAA CTGCATCCAC TAATGTGTCT
    GCCTCCGACC GATTCTCTGC CTCAAACTTT GGGAAGGAGA GATTTGTAAA GCTGCTTGAC
    CAGTTGCACA ATTCCTTACG CATTGACCTG TCCATGTATC GGAACAACTT CCCTGCTAGC
    AGCCCTGAAA GACTCCAAGA TCTCAAGTCC ACTGTAGATT TACTAACTAG CATAACATTT
    TTCCGGATGA AGGTACAAGA GCTTCAGAGC CCACCACGGG CAAGCCAAGT TGTTAAGGAC
    TGTGTGAAGG CATGCCTTAA CTCAACCTAC GAATATATCT TTAACAACTG CCATGAACTT
    TATAGCCGAG AATATCAAAG TGACCCTGCT AAAAAAGGGG AGGTACCCCC AGAAGAACAG
    GGGCCAAGTA TAAAGAACTT AGATTTTTGG TCCAAACTAA TCACATTGAT TGTGTCTATC
    ATAGAGGAAG ATAAGAACTC ATATACACCT TGTCTAAACC AGTTTCCTCA GGAGCTAAAT
    GTTGGTAAGA TTAGTGCTGA GGTCATGTGG AGTCTCTTTG CTCAAGACAT GAGATATGCT
    ATGGAAGAAC ACGACAAACA CCGCCTGTGT AAGAGTGCAG ACTACATGAA CTTGCATTTT
    AAGGTCAAGT GGTTGTACAA TGAATATGTA ACTGAGCTAC CATCTTTTAA GAGCAAGGTT
    CCAGAATATC CCGCCTGGTT TGAGCCTTTT GTAATTCAGT GGCTGGATGA AAATGAAGAG
    GTCTCCCGGG ATTTTCTGCA TGGAGCACTT GAAAGAGATA AGAAGGATGG GTTCCAGCAG
    ACCTCTGAAC ATGCCCTGTT CTCCTGCTCT GTGGTGGATG TTTTCTCACA ACTTAACCAG
    AGCTTTGAGA TCATTAAGAA GTTGGAGTGT CCAGACCCAC AAATTGTGGG GCATTACATG
    CGTAGATTTG CCAAGACGAT CAGTAATGTT CTGCTGCAGT ATGCTGAAAT TATTTCCAAA
    GATTTTGCAT CTCATTGCTC CAAAGAGAAG AAAGAAGAAA AAGTGCCCTG TATTCTTATG
    AACAACATCC AGCAACTTCG AGTGCAACTA GAAAAAATGT TTGAAGCAAT GGGAGGAAAG
    GAGCTGGATG CAGAAACAAG TGACATTCTT AAAGAGCTGC AGGTGAAACT CAACAATGTC
    CTAGATGAGC TAAGCAGAGT GTTTACCACC AGTTTTCAGG CACAAATTGA GGAGTGTGTG
    AAGCAGATGG GTGACATCCT GAGCCAGGTA AAAGGGACCG GAAATGTACC AGCCAGTAAC
    TGCAGCAGTG TGGCTCAGGA TGCAGATAAT GTATTGCAGC CTATCCTGGA TCCTCTGGAC
    AGCAACCTGA CACTATTTGC CAAGATCTGT GAAAAGACAG TCCTGAAGCG GGTACTAAAA
    GAACTCTGGA AGCTTGTGAT GAATACAATG GAGAAAACGA TTGTCCTTCC ACCACTAACA
    GACCAAACAA TGATAGGGAC GCTGCTGAGA AAACATGGCA AGGGAATAGA GAAGAGCAGG
    GTGAAGCTGC CGTACCACGC AGATGGAACC CACCTTATCA TTAATGCAGC CAAGGAACTG
    GGCCAGTTAT CCAAACTAAA GGATCACATG GTGCGAGAGG AAGCCAAGAG CCTCACCCCT
    AAGCAGTGTG CAGTGGTGGA GCTTGCACTT GATACAATTA AGCAATATTT CCATGCTGGA
    GGTGTTGGTC TCAAGAAGAC ATTCCTGGAG AAGAGTCCAG ACCTACAATC TCTACGCTAT
    GCACTTTCTC TTTACACACA AGCAACCGAT ACGCTAATCA AAACCTTCGT CCAGACACAG
    TCTGCTCAGG TTCATGGTGG TAAAGGTATT AGGTTTACAC TTAATGAAGA GACTTATCCA
    GAAAAGGGAT CTGGGGTGGA TGATTCTGTT GGGGAAGTTT CTATCCATGT GGAGTTATTT
    ACTCACCCAG GAACAGGCGA GCACAAGATC AGCGTAAAGG TTGTAGCGGC CAATGACCTT
    AAATGGCAGA CGTCAGGGAT TTTCCGTCCC TTTATTGAGG TAAACATGAT TGGTCCGAAT
    CTGAGTGACA AAAAAAGGAA ATTTACCACC AAGTCCAAGA ACAACAGCTG GGCCCCAAAA
    TATAACGAGA GCTTCCAATT TACCCTGGGT AATGAGACTG GTCCAGAGAG TTATGAGCTG
    CAAGTATGTG TGAAAGATTA CTGTTTTGCT CGGGAAGACC GCACAGTGGG AATGGCTGTC
    ATGCAACTGC GAGATCTGGC ACAAAGGGGT AGCTGTGCCT GTTGGCTCCC TCTTGGCCGC
    CGCATTCATA TGGATGAGAC AGGACTGACC ATCTTGCGCA TACTATCACA AAGAAACAAT
    GATGAGGTGG CCAAAGAGTT TGTCAAACTC AAATCTGACA CCCGCTCTCC AGAAGAGGGG
    AGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012810187.2
    CDS188..5413
    Translation

    Target ORF information:

    RefSeq Version XM_012954733.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis unc-13 homolog A (unc13a), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012954733.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGTCTTTGC TTTGCGTGGG AGTAAAAAAG GCCACGTTTG ATGGAGCACA AGAGAAGTTT 
    AATGCCTACG TAACCTTGAA GGTGCAAAAT GTGAAGAGCA CAACGATAGC GGTGCGGGGA
    AACCAGCCCT GCTGGGAACA GGATTTCATG TTTGAGATTA ATCGTTTAGA CCTTGGTCTA
    ACAGTGGAGG TCTGGAACAA AGGATTGATC TGGGACACCA TGGTGGGAAC AGTATGGATT
    CCTTTAAGGA CAATCCGACA ATCCAATGAG GAGGGTCCTG GGGAATGGCT GACACTTGAT
    TCACAGGTCA TAATGACAGA TAATGAGATT TGTGGTACCA AAGATCCAAC CTTCCACCGC
    ATCTTACTGG ATACCCGATT TGAACTTCCT CTAGATATAC CAGAAGAAGA AGCTCGATAC
    TGGGCAAAGA AACTTGAGCA ACTTAATGCA ATGAGGGACC AGGATGATTA TGTTTTTCAA
    GAAGAAATTC CGGAGAATCC TCTTCCCGTC CATGGATCTC AATGTTGTAA CTGGAATTAT
    TTCGGCTGGG GGGAGCAGCA CAATGAGGAT CCCGATAGTG CAGTGGATGA TCGGGATAGT
    GATTACCGCA GTGAAACCAG CAACAGTATC CCGCCCCCCT ACTACTCCAC CTCACAGCCT
    AATGCCTCTG TGCACCAGTA CCCTGTACGA CAGCAACACA GTTCTCATGG ATCTTACACT
    GATTCAGTGC ACAGCTATGA GATGGACTTC TCTGACCATC GTCCAATCAG GCGTTACGAT
    AGCGCAGACT CTGCCACTAA CGGGCGCAGT GATGTGGAGT CTGTCTCCGG TTCGAGCCGC
    CTCACCAGCC GGCAGCACTC TCTGGAGAGC CGGCAGTCCT TAGACCTGTC CGATAGCCCC
    ACTGGAAGTA GTCGATATGC TTCATCAGGG GAGATTAGTC GTGGCTCCTC ACAGCTCAGT
    GATGACTTTG ACCCAGATGA TGACAGCTTG CAAGGTTCAG AAATAGAGGA AGAAAGGGAT
    AGAGACTCTT ATCACTCCTG TCATAGTTCT GTCAGCTGCC GCAAGGACTC GCCAAATTGG
    GAAGGAGAGG AGGAGCCTGA AGTTTATAGT GAAGATGAGG AAGAATATCC TGACAGTAGG
    GGGCAGCTCT CTGACCAGGA GCTGTATGAT GATGACCAGC TTGAGGAAGA AGAGAAAAAA
    GGAAGATTAG CATATGGGCA GGAGGATTCC ATCTATCAGT CATTGGAGGC TGAGGAGAAG
    GAACCCCTGG ATATTCCATT GGAGAAGGAC AGGACACCAG AAGCTGTGCC AGAGGAGGTA
    GTAGAGCCAA CAGTACCAGC AGTCCAGTCT CCCCAAGCTG CAGACAGTTA CAGAGAAGAA
    GAACCTGGAA TGTCCAGGGC CAAATCTAAT TGGTTAAGAG CATTCAACAA GGTGCGCCTG
    CAGCTGCAAG AGGCACGAGG TGAAGGGACT GGGGAGATGT CCAAATCCCT CTGGTTTAAG
    GATGGGCCAG CTGGTGGTCT TATAATTATA GACAGTATGC CAGACATACG AAAGAGGAAA
    CCAATCCCGT TGGTCAGTGA CCTGGCAATG TCACTGGTTC AGTCACGGAA GGCTGGGATT
    ACTTCTGCTC TTGCAACAAG CACCTTAAAC AATGAAGAGC TAAAAAACCA TGTTTACAAA
    AAGACCCTGC AAGCTTTAAT ATACCCAATA TCCAGCACCA CTCCCCACAA CTTTGAAGTT
    TGGACTGCCA CAACTCCTAC CTATTGTTAT GAATGCGAAG GGCTTCTGTG GGGAATCGCT
    CGGCAGGGCA TGCGTTGCAC AGAGTGTGGT GTCAAGTGCC ATGAGAAGTG CCAGGATCTG
    CTGAATGCAG ATTGTCTTCA AAGAGCTGCA GAGAAGAGCT CAAAACATGG TGCAGAAGAC
    CGGACTCAGA ATATTATTAT GGTCTTGAAG GATCGTATGA AGATCCGGGA GAGGAACAAG
    CCAGAAATAT TTGAGCTGAT CCAGGAAGTA TTTGGGGTAT TAAAGGCTAC ACACGCTCAG
    CAGATAAAGG CTGTGAAACA GAGTGTGCTG GATGGTACTT CCAAATGGTC AGCAAAGATC
    AGCATAACTG TTGTATGTGC ACAGGGTCTA CAGGCCAAAG ATAAGACAGG CTCCAGTGAC
    CCATATGTTA CTGTTCAAGT TGGGAAAACC AAGAAAAGAA CCAAAACAAT CTATGGAAAT
    CTTAATCCTG TCTGGGAAGA GAATTTTCAC TTTGAATGCC ACAACTCCTC AGATCGTATT
    AAAGTACGCG TGTGGGATGA AGATGATGAT ATCAAATCTC GTGTGAAGCA GAGATTTAAA
    AGAGAATCAG ATGACTTCCT TGGACAAACA ATTATTGAAG TCAGAACACT TAGCGGAGAG
    ATGGATGTGT GGTATAACTT GGATAAGAGA ACAGATAAAT CCGCTGTCTC TGGGGCAATC
    CGTCTTCATA TAAGTGTGGA AATAAAAGGA GAGGAAAAGG TGGCTCCCTA TCACGTGCAA
    TACACATGTC TACATGAGAA CTTATTCCAT TACCTGACGG ATGTGCAGAA TAATGGTGTG
    GTCAAGATTC CAGATGCAAA AGGAGACGAC GCCTGGAAAG TTTATTTTGA TGAGACAGCC
    CAGGAAATTG TGGATGAATT TGCAATGAGA TATGGAGTGG AGTCCATATA TCAGGCCATG
    ACGCATTTTG CCTGCCTTTC ATCTAAATAT ATGTGTCCAG GAGTACCAGC TGTAATGAGT
    ACTCTTCTTG CCAACATTAA TGCTTACTAT GCGCACACAA CTGCATCCAC TAATGTGTCT
    GCCTCCGACC GATTCTCTGC CTCAAACTTT GGGAAGGAGA GATTTGTAAA GCTGCTTGAC
    CAGTTGCACA ATTCCTTACG CATTGACCTG TCCATGTATC GGAACAACTT CCCTGCTAGC
    AGCCCTGAAA GACTCCAAGA TCTCAAGTCC ACTGTAGATT TACTAACTAG CATAACATTT
    TTCCGGATGA AGGTACAAGA GCTTCAGAGC CCACCACGGG CAAGCCAAGT TGTTAAGGAC
    TGTGTGAAGG CATGCCTTAA CTCAACCTAC GAATATATCT TTAACAACTG CCATGAACTT
    TATAGCCGAG AATATCAAAG TGACCCTGCT AAAAAAGGGG AGGTACCCCC AGAAGAACAG
    GGGCCAAGTA TAAAGAACTT AGATTTTTGG TCCAAACTAA TCACATTGAT TGTGTCTATC
    ATAGAGGAAG ATAAGAACTC ATATACACCT TGTCTAAACC AGTTTCCTCA GGAGCTAAAT
    GTTGGTAAGA TTAGTGCTGA GGTCATGTGG AGTCTCTTTG CTCAAGACAT GAGATATGCT
    ATGGAAGAAC ACGACAAACA CCGCCTGTGT AAGAGTGCAG ACTACATGAA CTTGCATTTT
    AAGGTCAAGT GGTTGTACAA TGAATATGTA ACTGAGCTAC CATCTTTTAA GAGCAAGGTT
    CCAGAATATC CCGCCTGGTT TGAGCCTTTT GTAATTCAGT GGCTGGATGA AAATGAAGAG
    GTCTCCCGGG ATTTTCTGCA TGGAGCACTT GAAAGAGATA AGAAGGATGG GTTCCAGCAG
    ACCTCTGAAC ATGCCCTGTT CTCCTGCTCT GTGGTGGATG TTTTCTCACA ACTTAACCAG
    AGCTTTGAGA TCATTAAGAA GTTGGAGTGT CCAGACCCAC AAATTGTGGG GCATTACATG
    CGTAGATTTG CCAAGACGAT CAGTAATGTT CTGCTGCAGT ATGCTGAAAT TATTTCCAAA
    GATTTTGCAT CTCATTGCTC CAAAGAGAAG AAAGAAGAAA AAGTGCCCTG TATTCTTATG
    AACAACATCC AGCAACTTCG AGTGCAACTA GAAAAAATGT TTGAAGCAAT GGGAGGAAAG
    GAGCTGGATG CAGAAACAAG TGACATTCTT AAAGAGCTGC AGGTGAAACT CAACAATGTC
    CTAGATGAGC TAAGCAGAGT GTTTACCACC AGTTTTCAGG CACAAATTGA GGAGTGTGTG
    AAGCAGATGG GTGACATCCT GAGCCAGGTA AAAGGGACCG GAAATGTACC AGCCAGTAAC
    TGCAGCAGTG TGGCTCAGGA TGCAGATAAT GTATTGCAGC CTATCCTGGA TCCTCTGGAC
    AGCAACCTGA CACTATTTGC CAAGATCTGT GAAAAGACAG TCCTGAAGCG GGTACTAAAA
    GAACTCTGGA AGCTTGTGAT GAATACAATG GAGAAAACGA TTGTCCTTCC ACCACTAACA
    GACCAAACAA TGATAGGGAC GCTGCTGAGA AAACATGGCA AGGGAATAGA GAAGAGCAGG
    GTGAAGCTGC CGTACCACGC AGATGGAACC CACCTTATCA TTAATGCAGC CAAGGAACTG
    GGCCAGTTAT CCAAACTAAA GGATCACATG GTGCGAGAGG AAGCCAAGAG CCTCACCCCT
    AAGCAGTGTG CAGTGGTGGA GCTTGCACTT GATACAATTA AGCAATATTT CCATGCTGGA
    GGTGTTGGTC TCAAGAAGAC ATTCCTGGAG AAGAGTCCAG ACCTACAATC TCTACGCTAT
    GCACTTTCTC TTTACACACA AGCAACCGAT ACGCTAATCA AAACCTTCGT CCAGACACAG
    TCTGCTCAGG TTCATGGTGG TAAAGGTATT AGGTTTACAC TTAATGAAGA GACTTATCCA
    GAAAAGGGAT CTGGGGTGGA TGATTCTGTT GGGGAAGTTT CTATCCATGT GGAGTTATTT
    ACTCACCCAG GAACAGGCGA GCACAAGATC AGCGTAAAGG TTGTAGCGGC CAATGACCTT
    AAATGGCAGA CGTCAGGGAT TTTCCGTCCC TTTATTGAGG TAAACATGAT TGGTCCGAAT
    CTGAGTGACA AAAAAAGGAA ATTTACCACC AAGTCCAAGA ACAACAGCTG GGCCCCAAAA
    TATAACGAGA GCTTCCAATT TACCCTGGGT AATGAGACTG GTCCAGAGAG TTATGAGCTG
    CAAGTATGTG TGAAAGATTA CTGTTTTGCT CGGGAAGACC GCACAGTGGG AATGGCTGTC
    ATGCAACTGC GAGATCTGGC ACAAAGGGGT AGCTGTGCCT GTTGGCTCCC TCTTGGCCGC
    CGCATTCATA TGGATGAGAC AGGACTGACC ATCTTGCGCA TACTATCACA AAGAAACAAT
    GATGAGGTGG CCAAAGAGTT TGTCAAACTC AAATCTGACA CCCGCTCTCC AGAAGAGGGG
    AGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa48431
    Clone ID Related Accession (Same CDS sequence) XM_031904665.1
    Accession Version XM_031904665.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5190bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein unc-13 homolog A isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGTCTTTGC TTTGCGTGGG AGTAAAAAAG GCCACGTTTG ATGGAGCACA AGAGAAGTTT 
    AATGCCTACG TAACCTTGAA GGTGCAAAAT GTGAAGAGCA CAACGATAGC GGTGCGGGGA
    AACCAGCCCT GCTGGGAACA GGATTTCATG TTTGAGATTA ATCGTTTAGA CCTTGGTCTA
    ACAGTGGAGG TCTGGAACAA AGGATTGATC TGGGACACCA TGGTGGGAAC AGTATGGATT
    CCTTTAAGGA CAATCCGACA ATCCAATGAG GAGGGTCCTG GGGAATGGCT GACACTTGAT
    TCACAGGTCA TAATGACAGA TAATGAGATT TGTGGTACCA AAGATCCAAC CTTCCACCGC
    ATCTTACTGG ATACCCGATT TGAACTTCCT CTAGATATAC CAGAAGAAGA AGCTCGATAC
    TGGGCAAAGA AACTTGAGCA ACTTAATGCA ATGAGGGACC AGGATGATTA TGTTTTTCAA
    GAAGAAATTC CGGAGAATCC TCTTCCCGTC CATGGATCTC AATGTTGTAA CTGGAATTAT
    TTCGGCTGGG GGGAGCAGCA CAATGAGGAT CCCGATAGTG CAGTGGATGA TCGGGATAGT
    GATTACCGCA GTGAAACCAG CAACAGTATC CCGCCCCCCT ACTACTCCAC CTCACAGCCT
    AATGCCTCTG TGCACCAGTA CCCTGTACGA CAGCAACACA GTTCTCATGG ATCTTACACT
    GATTCAGTGC ACAGCTATGA GATGGACTTC TCTGACCATC GTCCAATCAG CAGGCGTTAC
    GATAGCGCAG ACTCTGCCAC TAACGGGCGC AGTGATGTGG AGTCTGTCTC CGGTTCGAGC
    CGCCTCACCA GCCGGCAGCA CTCTCTGGAG AGCCGGCAGT CCTTAGACCT GTCCGATAGC
    CCCACTGGAA GTAGTCGATA TGCTTCATCA GGGGAGATTA GTCGTGGCTC CTCACAGCTC
    AGTGATGACT TTGACCCAGA TGATGACAGC TTGCAAGGTT CAGAAATAGA GGAAGAAAGG
    GATAGAGACT CTTATCACTC CTGTCATAGT TCTGTCAGCT GCCGCAAGGA CTCGCCAAAT
    TGGGAAGGAG AGGAGGAGCC TGAAGTTTAT AGTGAAGATG AGGAAGAATA TCCTGACAGT
    AGGGGGCAGC TCTCTGACCA GGAGCTGTAT GATGATGACC AGCTTGAGGA AGAAGAGAAA
    AAAGGAAGAT TAGCATATGG GCAGGAGGAT TCCATCTATC AGTCATTGGA GGCTGAGGAG
    AAGGAACCCC TGGATATTCC ATTGGAGAAG GACAGGACAC CAGAAGCTGT GCCAGAGGAG
    GTAGTAGAGC CAACAGTACC AGCAGTCCAG TCTCCCCAAG CTGCAGACAG TTACAGAGAA
    GAAGAACCTG GAATGTCCAG GGCCAAATCT AATTGGTTAA GAGCATTCAA CAAGGTGCGC
    CTGCAGCTGC AAGAGGCACG AGGTGAAGGG ACTGGGGAGA TGTCCAAATC CCTCTGGTTT
    AAGGATGGGC CAGCTGGTGG TCTTATAATT ATAGACAGTA TGCCAGACAT ACGAAAGAGG
    AAACCAATCC CGTTGGTCAG TGACCTGGCA ATGTCACTGG TTCAGTCACG GAAGGCTGGG
    ATTACTTCTG CTCTTGCAAC AAGCACCTTA AACAATGAAG AGCTAAAAAA CCATGTTTAC
    AAAAAGACCC TGCAAGCTTT AATATACCCA ATATCCAGCA CCACTCCCCA CAACTTTGAA
    GTTTGGACTG CCACAACTCC TACCTATTGT TATGAATGCG AAGGGCTTCT GTGGGGAATC
    GCTCGGCAGG GCATGCGTTG CACAGAGTGT GGTGTCAAGT GCCATGAGAA GTGCCAGGAT
    CTGCTGAATG CAGATTGTCT TCAAAGAGCT GCAGAGAAGA GCTCAAAACA TGGTGCAGAA
    GACCGGACTC AGAATATTAT TATGGTCTTG AAGGATCGTA TGAAGATCCG GGAGAGGAAC
    AAGCCAGAAA TATTTGAGCT GATCCAGGAA GTATTTGGGG TATTAAAGGC TACACACGCT
    CAGCAGATAA AGGCTGTGAA ACAGAGTGTG CTGGATGGTA CTTCCAAATG GTCAGCAAAG
    ATCAGCATAA CTGTTGTATG TGCACAGGGT CTACAGGCCA AAGATAAGAC AGGCTCCAGT
    GACCCATATG TTACTGTTCA AGTTGGGAAA ACCAAGAAAA GAACCAAAAC AATCTATGGA
    AATCTTAATC CTGTCTGGGA AGAGAATTTT CACTTTGAAT GCCACAACTC CTCAGATCGT
    ATTAAAGTAC GCGTGTGGGA TGAAGATGAT GATATCAAAT CTCGTGTGAA GCAGAGATTT
    AAAAGAGAAT CAGATGACTT CCTTGGACAA ACAATTATTG AAGTCAGAAC ACTTAGCGGA
    GAGATGGATG TGTGGTATAA CTTGGATAAG AGAACAGATA AATCCGCTGT CTCTGGGGCA
    ATCCGTCTTC ATATAAGTGT GGAAATAAAA GGAGAGGAAA AGGTGGCTCC CTATCACGTG
    CAATACACAT GTCTACATGA GAACTTATTC CATTACCTGA CGGATGTGCA GAATAATGGT
    GTGGTCAAGA TTCCAGATGC AAAAGGAGAC GACGCCTGGA AAGTTTATTT TGATGAGACA
    GCCCAGGAAA TTGTGGATGA ATTTGCAATG AGATATGGAG TGGAGTCCAT ATATCAGGCC
    ATGACGCATT TTGCCTGCCT TTCATCTAAA TATATGTGTC CAGGAGTACC AGCTGTAATG
    AGTACTCTTC TTGCCAACAT TAATGCTTAC TATGCGCACA CAACTGCATC CACTAATGTG
    TCTGCCTCCG ACCGATTCTC TGCCTCAAAC TTTGGGAAGG AGAGATTTGT AAAGCTGCTT
    GACCAGTTGC ACAATTCCTT ACGCATTGAC CTGTCCATGT ATCGGAACAA CTTCCCTGCT
    AGCAGCCCTG AAAGACTCCA AGATCTCAAG TCCACTGTAG ATTTACTAAC TAGCATAACA
    TTTTTCCGGA TGAAGGTACA AGAGCTTCAG AGCCCACCAC GGGCAAGCCA AGTTGTTAAG
    GACTGTGTGA AGGCATGCCT TAACTCAACC TACGAATATA TCTTTAACAA CTGCCATGAA
    CTTTATAGCC GAGAATATCA AAGTGACCCT GCTAAAAAAG GGGAGGTACC CCCAGAAGAA
    CAGGGGCCAA GTATAAAGAA CTTAGATTTT TGGTCCAAAC TAATCACATT GATTGTGTCT
    ATCATAGAGG AAGATAAGAA CTCATATACA CCTTGTCTAA ACCAGTTTCC TCAGGAGCTA
    AATGTTGGTA AGATTAGTGC TGAGGTCATG TGGAGTCTCT TTGCTCAAGA CATGAGATAT
    GCTATGGAAG AACACGACAA ACACCGCCTG TGTAAGAGTG CAGACTACAT GAACTTGCAT
    TTTAAGGTCA AGTGGTTGTA CAATGAATAT GTAACTGAGC TACCATCTTT TAAGAGCAAG
    GTTCCAGAAT ATCCCGCCTG GTTTGAGCCT TTTGTAATTC AGTGGCTGGA TGAAAATGAA
    GAGGTCTCCC GGGATTTTCT GCATGGAGCA CTTGAAAGAG ATAAGAAGGA TGGGTTCCAG
    CAGACCTCTG AACATGCCCT GTTCTCCTGC TCTGTGGTGG ATGTTTTCTC ACAACTTAAC
    CAGAGCTTTG AGATCATTAA GAAGTTGGAG TGTCCAGACC CACAAATTGT GGGGCATTAC
    ATGCGTAGAT TTGCCAAGAC GATCAGTAAT GTTCTGCTGC AGTATGCTGA AATTATTTCC
    AAAGATTTTG CATCTCATTG CTCCAAAGAG AAGAAAGAAG AAAAAGTGCC CTGTATTCTT
    ATGAACAACA TCCAGCAACT TCGAGTGCAA CTAGAAAAAA TGTTTGAAGC AATGGGAGGA
    AAGGAGCTGG ATGCAGAAAC AAGTGACATT CTTAAAGAGC TGCAGGTGAA ACTCAACAAT
    GTCCTAGATG AGCTAAGCAG AGTGTTTACC ACCAGTTTTC AGGCACAAAT TGAGGAGTGT
    GTGAAGCAGA TGGGTGACAT CCTGAGCCAG GTAAAAGGGA CCGGAAATGT ACCAGCCAGT
    AACTGCAGCA GTGTGGCTCA GGATGCAGAT AATGTATTGC AGCCTATCCT GGATCCTCTG
    GACAGCAACC TGACACTATT TGCCAAGATC TGTGAAAAGA CAGTCCTGAA GCGGGTACTA
    AAAGAACTCT GGAAGCTTGT GATGAATACA ATGGAGAAAA CGATTGTCCT TCCACCACTA
    ACAGACCAAA CAATGATAAG CAGGGTGAAG CTGCCGTACC ACGCAGATGG AACCCACCTT
    ATCATTAATG CAGCCAAGGA ACTGGGCCAG TTATCCAAAC TAAAGGATCA CATGGTGCGA
    GAGGAAGCCA AGAGCCTCAC CCCTAAGCAG TGTGCAGTGG TGGAGCTTGC ACTTGATACA
    ATTAAGCAAT ATTTCCATGC TGGAGGTGTT GGTCTCAAGA AGACATTCCT GGAGAAGAGT
    CCAGACCTAC AATCTCTACG CTATGCACTT TCTCTTTACA CACAAGCAAC CGATACGCTA
    ATCAAAACCT TCGTCCAGAC ACAGTCTGCT CAGGTTCATG GTGGTAAAGG TATTAGGTTT
    ACACTTAATG AAGAGACTTA TCCAGAAAAG GGATCTGGGG TGGATGATTC TGTTGGGGAA
    GTTTCTATCC ATGTGGAGTT ATTTACTCAC CCAGGAACAG GCGAGCACAA GATCAGCGTA
    AAGGTTGTAG CGGCCAATGA CCTTAAATGG CAGACGTCAG GGATTTTCCG TCCCTTTATT
    GAGGTAAACA TGATTGGTCC GAATCTGAGT GACAAAAAAA GGAAATTTAC CACCAAGTCC
    AAGAACAACA GCTGGGCCCC AAAATATAAC GAGAGCTTCC AATTTACCCT GGGTAATGAG
    ACTGGTCCAG AGAGTTATGA GCTGCAAGTA TGTGTGAAAG ATTACTGTTT TGCTCGGGAA
    GACCGCACAG TGGGAATGGC TGTCATGCAA CTGCGAGATC TGGCACAAAG GGGTAGCTGT
    GCCTGTTGGC TCCCTCTTGG CCGCCGCATT CATATGGATG AGACAGGACT GACCATCTTG
    CGCATACTAT CACAAAGAAA CAATGATGAG GTGGCCAAAG AGTTTGTCAA ACTCAAATCT
    GACACCCGCT CTCCAGAAGA GGGGAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031760525.1
    CDS188..5377
    Translation

    Target ORF information:

    RefSeq Version XM_031904665.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis unc-13 homolog A (unc13a), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031904665.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGTCTTTGC TTTGCGTGGG AGTAAAAAAG GCCACGTTTG ATGGAGCACA AGAGAAGTTT 
    AATGCCTACG TAACCTTGAA GGTGCAAAAT GTGAAGAGCA CAACGATAGC GGTGCGGGGA
    AACCAGCCCT GCTGGGAACA GGATTTCATG TTTGAGATTA ATCGTTTAGA CCTTGGTCTA
    ACAGTGGAGG TCTGGAACAA AGGATTGATC TGGGACACCA TGGTGGGAAC AGTATGGATT
    CCTTTAAGGA CAATCCGACA ATCCAATGAG GAGGGTCCTG GGGAATGGCT GACACTTGAT
    TCACAGGTCA TAATGACAGA TAATGAGATT TGTGGTACCA AAGATCCAAC CTTCCACCGC
    ATCTTACTGG ATACCCGATT TGAACTTCCT CTAGATATAC CAGAAGAAGA AGCTCGATAC
    TGGGCAAAGA AACTTGAGCA ACTTAATGCA ATGAGGGACC AGGATGATTA TGTTTTTCAA
    GAAGAAATTC CGGAGAATCC TCTTCCCGTC CATGGATCTC AATGTTGTAA CTGGAATTAT
    TTCGGCTGGG GGGAGCAGCA CAATGAGGAT CCCGATAGTG CAGTGGATGA TCGGGATAGT
    GATTACCGCA GTGAAACCAG CAACAGTATC CCGCCCCCCT ACTACTCCAC CTCACAGCCT
    AATGCCTCTG TGCACCAGTA CCCTGTACGA CAGCAACACA GTTCTCATGG ATCTTACACT
    GATTCAGTGC ACAGCTATGA GATGGACTTC TCTGACCATC GTCCAATCAG CAGGCGTTAC
    GATAGCGCAG ACTCTGCCAC TAACGGGCGC AGTGATGTGG AGTCTGTCTC CGGTTCGAGC
    CGCCTCACCA GCCGGCAGCA CTCTCTGGAG AGCCGGCAGT CCTTAGACCT GTCCGATAGC
    CCCACTGGAA GTAGTCGATA TGCTTCATCA GGGGAGATTA GTCGTGGCTC CTCACAGCTC
    AGTGATGACT TTGACCCAGA TGATGACAGC TTGCAAGGTT CAGAAATAGA GGAAGAAAGG
    GATAGAGACT CTTATCACTC CTGTCATAGT TCTGTCAGCT GCCGCAAGGA CTCGCCAAAT
    TGGGAAGGAG AGGAGGAGCC TGAAGTTTAT AGTGAAGATG AGGAAGAATA TCCTGACAGT
    AGGGGGCAGC TCTCTGACCA GGAGCTGTAT GATGATGACC AGCTTGAGGA AGAAGAGAAA
    AAAGGAAGAT TAGCATATGG GCAGGAGGAT TCCATCTATC AGTCATTGGA GGCTGAGGAG
    AAGGAACCCC TGGATATTCC ATTGGAGAAG GACAGGACAC CAGAAGCTGT GCCAGAGGAG
    GTAGTAGAGC CAACAGTACC AGCAGTCCAG TCTCCCCAAG CTGCAGACAG TTACAGAGAA
    GAAGAACCTG GAATGTCCAG GGCCAAATCT AATTGGTTAA GAGCATTCAA CAAGGTGCGC
    CTGCAGCTGC AAGAGGCACG AGGTGAAGGG ACTGGGGAGA TGTCCAAATC CCTCTGGTTT
    AAGGATGGGC CAGCTGGTGG TCTTATAATT ATAGACAGTA TGCCAGACAT ACGAAAGAGG
    AAACCAATCC CGTTGGTCAG TGACCTGGCA ATGTCACTGG TTCAGTCACG GAAGGCTGGG
    ATTACTTCTG CTCTTGCAAC AAGCACCTTA AACAATGAAG AGCTAAAAAA CCATGTTTAC
    AAAAAGACCC TGCAAGCTTT AATATACCCA ATATCCAGCA CCACTCCCCA CAACTTTGAA
    GTTTGGACTG CCACAACTCC TACCTATTGT TATGAATGCG AAGGGCTTCT GTGGGGAATC
    GCTCGGCAGG GCATGCGTTG CACAGAGTGT GGTGTCAAGT GCCATGAGAA GTGCCAGGAT
    CTGCTGAATG CAGATTGTCT TCAAAGAGCT GCAGAGAAGA GCTCAAAACA TGGTGCAGAA
    GACCGGACTC AGAATATTAT TATGGTCTTG AAGGATCGTA TGAAGATCCG GGAGAGGAAC
    AAGCCAGAAA TATTTGAGCT GATCCAGGAA GTATTTGGGG TATTAAAGGC TACACACGCT
    CAGCAGATAA AGGCTGTGAA ACAGAGTGTG CTGGATGGTA CTTCCAAATG GTCAGCAAAG
    ATCAGCATAA CTGTTGTATG TGCACAGGGT CTACAGGCCA AAGATAAGAC AGGCTCCAGT
    GACCCATATG TTACTGTTCA AGTTGGGAAA ACCAAGAAAA GAACCAAAAC AATCTATGGA
    AATCTTAATC CTGTCTGGGA AGAGAATTTT CACTTTGAAT GCCACAACTC CTCAGATCGT
    ATTAAAGTAC GCGTGTGGGA TGAAGATGAT GATATCAAAT CTCGTGTGAA GCAGAGATTT
    AAAAGAGAAT CAGATGACTT CCTTGGACAA ACAATTATTG AAGTCAGAAC ACTTAGCGGA
    GAGATGGATG TGTGGTATAA CTTGGATAAG AGAACAGATA AATCCGCTGT CTCTGGGGCA
    ATCCGTCTTC ATATAAGTGT GGAAATAAAA GGAGAGGAAA AGGTGGCTCC CTATCACGTG
    CAATACACAT GTCTACATGA GAACTTATTC CATTACCTGA CGGATGTGCA GAATAATGGT
    GTGGTCAAGA TTCCAGATGC AAAAGGAGAC GACGCCTGGA AAGTTTATTT TGATGAGACA
    GCCCAGGAAA TTGTGGATGA ATTTGCAATG AGATATGGAG TGGAGTCCAT ATATCAGGCC
    ATGACGCATT TTGCCTGCCT TTCATCTAAA TATATGTGTC CAGGAGTACC AGCTGTAATG
    AGTACTCTTC TTGCCAACAT TAATGCTTAC TATGCGCACA CAACTGCATC CACTAATGTG
    TCTGCCTCCG ACCGATTCTC TGCCTCAAAC TTTGGGAAGG AGAGATTTGT AAAGCTGCTT
    GACCAGTTGC ACAATTCCTT ACGCATTGAC CTGTCCATGT ATCGGAACAA CTTCCCTGCT
    AGCAGCCCTG AAAGACTCCA AGATCTCAAG TCCACTGTAG ATTTACTAAC TAGCATAACA
    TTTTTCCGGA TGAAGGTACA AGAGCTTCAG AGCCCACCAC GGGCAAGCCA AGTTGTTAAG
    GACTGTGTGA AGGCATGCCT TAACTCAACC TACGAATATA TCTTTAACAA CTGCCATGAA
    CTTTATAGCC GAGAATATCA AAGTGACCCT GCTAAAAAAG GGGAGGTACC CCCAGAAGAA
    CAGGGGCCAA GTATAAAGAA CTTAGATTTT TGGTCCAAAC TAATCACATT GATTGTGTCT
    ATCATAGAGG AAGATAAGAA CTCATATACA CCTTGTCTAA ACCAGTTTCC TCAGGAGCTA
    AATGTTGGTA AGATTAGTGC TGAGGTCATG TGGAGTCTCT TTGCTCAAGA CATGAGATAT
    GCTATGGAAG AACACGACAA ACACCGCCTG TGTAAGAGTG CAGACTACAT GAACTTGCAT
    TTTAAGGTCA AGTGGTTGTA CAATGAATAT GTAACTGAGC TACCATCTTT TAAGAGCAAG
    GTTCCAGAAT ATCCCGCCTG GTTTGAGCCT TTTGTAATTC AGTGGCTGGA TGAAAATGAA
    GAGGTCTCCC GGGATTTTCT GCATGGAGCA CTTGAAAGAG ATAAGAAGGA TGGGTTCCAG
    CAGACCTCTG AACATGCCCT GTTCTCCTGC TCTGTGGTGG ATGTTTTCTC ACAACTTAAC
    CAGAGCTTTG AGATCATTAA GAAGTTGGAG TGTCCAGACC CACAAATTGT GGGGCATTAC
    ATGCGTAGAT TTGCCAAGAC GATCAGTAAT GTTCTGCTGC AGTATGCTGA AATTATTTCC
    AAAGATTTTG CATCTCATTG CTCCAAAGAG AAGAAAGAAG AAAAAGTGCC CTGTATTCTT
    ATGAACAACA TCCAGCAACT TCGAGTGCAA CTAGAAAAAA TGTTTGAAGC AATGGGAGGA
    AAGGAGCTGG ATGCAGAAAC AAGTGACATT CTTAAAGAGC TGCAGGTGAA ACTCAACAAT
    GTCCTAGATG AGCTAAGCAG AGTGTTTACC ACCAGTTTTC AGGCACAAAT TGAGGAGTGT
    GTGAAGCAGA TGGGTGACAT CCTGAGCCAG GTAAAAGGGA CCGGAAATGT ACCAGCCAGT
    AACTGCAGCA GTGTGGCTCA GGATGCAGAT AATGTATTGC AGCCTATCCT GGATCCTCTG
    GACAGCAACC TGACACTATT TGCCAAGATC TGTGAAAAGA CAGTCCTGAA GCGGGTACTA
    AAAGAACTCT GGAAGCTTGT GATGAATACA ATGGAGAAAA CGATTGTCCT TCCACCACTA
    ACAGACCAAA CAATGATAAG CAGGGTGAAG CTGCCGTACC ACGCAGATGG AACCCACCTT
    ATCATTAATG CAGCCAAGGA ACTGGGCCAG TTATCCAAAC TAAAGGATCA CATGGTGCGA
    GAGGAAGCCA AGAGCCTCAC CCCTAAGCAG TGTGCAGTGG TGGAGCTTGC ACTTGATACA
    ATTAAGCAAT ATTTCCATGC TGGAGGTGTT GGTCTCAAGA AGACATTCCT GGAGAAGAGT
    CCAGACCTAC AATCTCTACG CTATGCACTT TCTCTTTACA CACAAGCAAC CGATACGCTA
    ATCAAAACCT TCGTCCAGAC ACAGTCTGCT CAGGTTCATG GTGGTAAAGG TATTAGGTTT
    ACACTTAATG AAGAGACTTA TCCAGAAAAG GGATCTGGGG TGGATGATTC TGTTGGGGAA
    GTTTCTATCC ATGTGGAGTT ATTTACTCAC CCAGGAACAG GCGAGCACAA GATCAGCGTA
    AAGGTTGTAG CGGCCAATGA CCTTAAATGG CAGACGTCAG GGATTTTCCG TCCCTTTATT
    GAGGTAAACA TGATTGGTCC GAATCTGAGT GACAAAAAAA GGAAATTTAC CACCAAGTCC
    AAGAACAACA GCTGGGCCCC AAAATATAAC GAGAGCTTCC AATTTACCCT GGGTAATGAG
    ACTGGTCCAG AGAGTTATGA GCTGCAAGTA TGTGTGAAAG ATTACTGTTT TGCTCGGGAA
    GACCGCACAG TGGGAATGGC TGTCATGCAA CTGCGAGATC TGGCACAAAG GGGTAGCTGT
    GCCTGTTGGC TCCCTCTTGG CCGCCGCATT CATATGGATG AGACAGGACT GACCATCTTG
    CGCATACTAT CACAAAGAAA CAATGATGAG GTGGCCAAAG AGTTTGTCAA ACTCAAATCT
    GACACCCGCT CTCCAGAAGA GGGGAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa48432
    Clone ID Related Accession (Same CDS sequence) XM_031904666.1
    Accession Version XM_031904666.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5172bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein unc-13 homolog A isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGTCTTTGC TTTGCGTGGG AGTAAAAAAG GCCACGTTTG ATGGAGCACA AGAGAAGTTT 
    AATGCCTACG TAACCTTGAA GGTGCAAAAT GTGAAGAGCA CAACGATAGC GGTGCGGGGA
    AACCAGCCCT GCTGGGAACA GGATTTCATG TTTGAGATTA ATCGTTTAGA CCTTGGTCTA
    ACAGTGGAGG TCTGGAACAA AGGATTGATC TGGGACACCA TGGTGGGAAC AGTATGGATT
    CCTTTAAGGA CAATCCGACA ATCCAATGAG GAGGGTCCTG GGGAATGGCT GACACTTGAT
    TCACAGGTCA TAATGACAGA TAATGAGATT TGTGGTACCA AAGATCCAAC CTTCCACCGC
    ATCTTACTGG ATACCCGATT TGAACTTCCT CTAGATATAC CAGAAGAAGA AGCTCGATAC
    TGGGCAAAGA AACTTGAGCA ACTTAATGCA ATGAGGGACC AGGATGATTA TGTTTTTCAA
    GAAGAAATTC CGGAGAATCC TCTTCCCGTC CATGGATCTC AATGTTGTAA CTGGAATTAT
    TTCGGCTGGG GGGAGCAGCA CAATGAGGAT CCCGATAGTG CAGTGGATGA TCGGGATAGT
    GATTACCGCA GTGAAACCAG CAACAGTATC CCGCCCCCCT ACTACTCCAC CTCACAGCCT
    AATGCCTCTG TGCACCAGTA CCCTGTACGA CAGCAACACA GTTCTCATGG ATCTTACACT
    GATTCAGTGC ACAGCTATGA GATGGACTTC TCTGACCATC GTCCAATCAG CAGGCGTTAC
    GATAGCGCAG ACTCTGCCAC TAACGGGCGC AGTGATGTGG AGTCTGTCTC CGGTTCGAGC
    CGCCTCACCA GCCGGCAGCA CTCTCTGGAG AGCCGGCAGT CCTTAGACCT GTCCGATAGC
    CCCACTGGAA GTAGTCGATA TGCTTCATCA GGGGAGATTA GTCGTGGCTC CTCACAGCTC
    AGTGATGACT TTGACCCAGA TGATGACAGC TTGCAAGGTT CAGAAATAGA GGAAGAAAGG
    GATAGAGACT CTTATCACTC CTGTCATAGT TCTGTCAGCT GCCGCAAGGA CTCGCCAAAT
    TGGGAAGGAG AGGAGGAGCC TGAAGTTTAT AGTGAAGATG AGGAAGAATA TCCTGACAGT
    AGGGGGCAGC TCTCTGACCA GGAGCTGTAT GATGATGACC AGCTTGAGGA AGAAGAGAAA
    AAAGGAAGAT TAGCATATGG GCAGGAGGAT TCCATCTATC AGTCATTGGA GGCTGAGGAG
    AAGGAACCCC TGGATATTCC ATTGGAGAAG GACAGGACAC CAGAAGCTGT GCCAGAGGAG
    GTAGTAGAGC CAACAGTACC AGCAGTCCAG TCTCCCCAAG CTGCAGACAG TTACAGAGAA
    GAAGAACCTG GAATGTCCAG GGCCAAATCT AATTGGTTAA GAGCATTCAA CAAGGTGCGC
    CTGCAGCTGC AAGAGGCACG AGGTGAAGGG ACTGGGGAGA TGTCCAAATC CCTCTGGTTT
    AAGGATGGGC CAGCTGGTGG TCTTATAATT ATAGACAGTA TGCCAGACAT ACGAAAGAGG
    AAACCAATCC CGTTGGTCAG TGACCTGGCA ATGTCACTGG TTCAGTCACG GAAGGCTGGG
    ATTACTTCTG CTCTTGCAAC AAGCACCTTA AACAATGAAG AGCTAAAAAA CCATGTTTAC
    AAAAAGACCC TGCAAGCTTT AATATACCCA ATATCCAGCA CCACTCCCCA CAACTTTGAA
    GTTTGGACTG CCACAACTCC TACCTATTGT TATGAATGCG AAGGGCTTCT GTGGGGAATC
    GCTCGGCAGG GCATGCGTTG CACAGAGTGT GGTGTCAAGT GCCATGAGAA GTGCCAGGAT
    CTGCTGAATG CAGATTGTCT TCAAAGAGCT GCAGAGAAGA GCTCAAAACA TGGTGCAGAA
    GACCGGACTC AGAATATTAT TATGGTCTTG AAGGATCGTA TGAAGATCCG GGAGAGGAAC
    AAGCCAGAAA TATTTGAGCT GATCCAGGAA GTATTTGGGG TATTAAAGGC TACACACGCT
    CAGCAGATAA AGGCTGTGAA ACAGAGTGTG CTGGATGGTA CTTCCAAATG GTCAGCAAAG
    ATCAGCATAA CTGTTGTATG TGCACAGGGT CTACAGGCCA AAGATAAGAC AGGCTCCAGT
    GACCCATATG TTACTGTTCA AGTTGGGAAA ACCAAGAAAA GAACCAAAAC AATCTATGGA
    AATCTTAATC CTGTCTGGGA AGAGAATTTT CACTTTGAAT GCCACAACTC CTCAGATCGT
    ATTAAAGTAC GCGTGTGGGA TGAAGATGAT GATATCAAAT CTCGTGTGAA GCAGAGATTT
    AAAAGAGAAT CAGATGACTT CCTTGGACAA ACAATTATTG AAGTCAGAAC ACTTAGCGGA
    GAGATGGATG TGTGGTATAA CTTGGATAAG AGAACAGATA AATCCGCTGT CTCTGGGGCA
    ATCCGTCTTC ATATAAGTGT GGAAATAAAA GGAGAGGAAA AGGTGGCTCC CTATCACGTG
    CAATACACAT GTCTACATGA GAACTTATTC CATTACCTGA CGGATGTGCA GAATAATGGT
    GTGGTCAAGA TTCCAGATGC AAAAGGAGAC GACGCCTGGA AAGTTTATTT TGATGAGACA
    GCCCAGGAAA TTGTGGATGA ATTTGCAATG AGATATGGAG TGGAGTCCAT ATATCAGGCC
    ATGACGCATT TTGCCTGCCT TTCATCTAAA TATATGTGTC CAGGAGTACC AGCTGTAATG
    AGTACTCTTC TTGCCAACAT TAATGCTTAC TATGCGCACA CAACTGCATC CACTAATGTG
    TCTGCCTCCG ACCGATTCTC TGCCTCAAAC TTTGGGAAGG AGAGATTTGT AAAGCTGCTT
    GACCAGTTGC ACAATTCCTT ACGCATTGAC CTGTCCATGT ATCGGAACAA CTTCCCTGCT
    AGCAGCCCTG AAAGACTCCA AGATCTCAAG TCCACTGTAG ATTTACTAAC TAGCATAACA
    TTTTTCCGGA TGAAGGTACA AGAGCTTCAG AGCCCACCAC GGGCAAGCCA AGTTGTTAAG
    GACTGTGTGA AGGCATGCCT TAACTCAACC TACGAATATA TCTTTAACAA CTGCCATGAA
    CTTTATAGCC GAGAATATCA AAGTGACCCT GCTAAAAAAG GGGAGGTACC CCCAGAAGAA
    CAGGGGCCAA GTATAAAGAA CTTAGATTTT TGGTCCAAAC TAATCACATT GATTGTGTCT
    ATCATAGAGG AAGATAAGAA CTCATATACA CCTTGTCTAA ACCAGTTTCC TCAGGAGCTA
    AATGTTGGTA AGATTAGTGC TGAGGTCATG TGGAGTCTCT TTGCTCAAGA CATGAGATAT
    GCTATGGAAG AACACGACAA ACACCGCCTG TGTAAGAGTG CAGACTACAT GAACTTGCAT
    TTTAAGGTCA AGTGGTTGTA CAATGAATAT GTAACTGAGC TACCATCTTT TAAGAGCAAG
    GTTCCAGAAT ATCCCGCCTG GTTTGAGCCT TTTGTAATTC AGTGGCTGGA TGAAAATGAA
    GAGGTCTCCC GGGATTTTCT GCATGGAGCA CTTGAAAGAG ATAAGAAGGA TGGGTTCCAG
    CAGACCTCTG AACATGCCCT GTTCTCCTGC TCTGTGGTGG ATGTTTTCTC ACAACTTAAC
    CAGAGCTTTG AGATCATTAA GAAGTTGGAG TGTCCAGACC CACAAATTGT GGGGCATTAC
    ATGCGTAGAT TTGCCAAGAC GATCAGTAAT GTTCTGCTGC AGTATGCTGA AATTATTTCC
    AAAGATTTTG CATCTCATTG CTCCAAAGAG AAGAAAGAAG AAAAAGTGCC CTGTATTCTT
    ATGAACAACA TCCAGCAACT TCGAGTGCAA CTAGAAAAAA TGTTTGAAGC AATGGGAGGA
    AAGGAGCTGG ATGCAGAAAC AAGTGACATT CTTAAAGAGC TGCAGGTGAA ACTCAACAAT
    GTCCTAGATG AGCTAAGCAG AGTGTTTACC ACCAGTTTTC AGGCACAAAT TGAGGAGTGT
    GTGAAGCAGA TGGGTGACAT CCTGAGCCAG GTAAAAGGGA CCGGAAATGT ACCAGCCAGT
    AACTGCAGCA GTGTGGCTCA GGATGCAGAT AATGTATTGC AGCCTATCCT GGATCCTCTG
    GACAGCAACC TGACACTATT TGCCAAGATC TGTGAAAAGA CAGTCCTGAA GCGGGTACTA
    AAAGAACTCT GGAAGCTTGT GATGAATACA ATGGAGAAAA CGATTGTCCT TCCACCACTA
    ACAGACCAAA CAATGATAGG GACGCTGCTG AGAAAACATG GCAAGGGAAT AGAGAAGAGC
    AGGGTGAAGC TGCCGTACCA CGCAGATGGA ACCCACCTTA TCATTAATGC AGCCAAGGAA
    CTGGGCCAGT TATCCAAACT AAAGGATCAC ATGGTGCGAG AGGAAGCCAA GAGCCTCACC
    CCTAAGCAGT GTGCAGTGGT GGAGCTTGCA CTTGATACAA TTAAGCAATA TTTCCATGCT
    GGAGGTGTTG GTCTCAAGAA GACATTCCTG GAGAAGAGTC CAGACCTACA ATCTCTACGC
    TATGCACTTT CTCTTTACAC ACAAGCAACC GATACGCTAA TCAAAACCTT CGTCCAGACA
    CAGTCTGCTC AGGGATCTGG GGTGGATGAT TCTGTTGGGG AAGTTTCTAT CCATGTGGAG
    TTATTTACTC ACCCAGGAAC AGGCGAGCAC AAGATCAGCG TAAAGGTTGT AGCGGCCAAT
    GACCTTAAAT GGCAGACGTC AGGGATTTTC CGTCCCTTTA TTGAGGTAAA CATGATTGGT
    CCGAATCTGA GTGACAAAAA AAGGAAATTT ACCACCAAGT CCAAGAACAA CAGCTGGGCC
    CCAAAATATA ACGAGAGCTT CCAATTTACC CTGGGTAATG AGACTGGTCC AGAGAGTTAT
    GAGCTGCAAG TATGTGTGAA AGATTACTGT TTTGCTCGGG AAGACCGCAC AGTGGGAATG
    GCTGTCATGC AACTGCGAGA TCTGGCACAA AGGGGTAGCT GTGCCTGTTG GCTCCCTCTT
    GGCCGCCGCA TTCATATGGA TGAGACAGGA CTGACCATCT TGCGCATACT ATCACAAAGA
    AACAATGATG AGGTGGCCAA AGAGTTTGTC AAACTCAAAT CTGACACCCG CTCTCCAGAA
    GAGGGGAGCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031760526.1
    CDS187..5358
    Translation

    Target ORF information:

    RefSeq Version XM_031904666.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis unc-13 homolog A (unc13a), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031904666.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGTCTTTGC TTTGCGTGGG AGTAAAAAAG GCCACGTTTG ATGGAGCACA AGAGAAGTTT 
    AATGCCTACG TAACCTTGAA GGTGCAAAAT GTGAAGAGCA CAACGATAGC GGTGCGGGGA
    AACCAGCCCT GCTGGGAACA GGATTTCATG TTTGAGATTA ATCGTTTAGA CCTTGGTCTA
    ACAGTGGAGG TCTGGAACAA AGGATTGATC TGGGACACCA TGGTGGGAAC AGTATGGATT
    CCTTTAAGGA CAATCCGACA ATCCAATGAG GAGGGTCCTG GGGAATGGCT GACACTTGAT
    TCACAGGTCA TAATGACAGA TAATGAGATT TGTGGTACCA AAGATCCAAC CTTCCACCGC
    ATCTTACTGG ATACCCGATT TGAACTTCCT CTAGATATAC CAGAAGAAGA AGCTCGATAC
    TGGGCAAAGA AACTTGAGCA ACTTAATGCA ATGAGGGACC AGGATGATTA TGTTTTTCAA
    GAAGAAATTC CGGAGAATCC TCTTCCCGTC CATGGATCTC AATGTTGTAA CTGGAATTAT
    TTCGGCTGGG GGGAGCAGCA CAATGAGGAT CCCGATAGTG CAGTGGATGA TCGGGATAGT
    GATTACCGCA GTGAAACCAG CAACAGTATC CCGCCCCCCT ACTACTCCAC CTCACAGCCT
    AATGCCTCTG TGCACCAGTA CCCTGTACGA CAGCAACACA GTTCTCATGG ATCTTACACT
    GATTCAGTGC ACAGCTATGA GATGGACTTC TCTGACCATC GTCCAATCAG CAGGCGTTAC
    GATAGCGCAG ACTCTGCCAC TAACGGGCGC AGTGATGTGG AGTCTGTCTC CGGTTCGAGC
    CGCCTCACCA GCCGGCAGCA CTCTCTGGAG AGCCGGCAGT CCTTAGACCT GTCCGATAGC
    CCCACTGGAA GTAGTCGATA TGCTTCATCA GGGGAGATTA GTCGTGGCTC CTCACAGCTC
    AGTGATGACT TTGACCCAGA TGATGACAGC TTGCAAGGTT CAGAAATAGA GGAAGAAAGG
    GATAGAGACT CTTATCACTC CTGTCATAGT TCTGTCAGCT GCCGCAAGGA CTCGCCAAAT
    TGGGAAGGAG AGGAGGAGCC TGAAGTTTAT AGTGAAGATG AGGAAGAATA TCCTGACAGT
    AGGGGGCAGC TCTCTGACCA GGAGCTGTAT GATGATGACC AGCTTGAGGA AGAAGAGAAA
    AAAGGAAGAT TAGCATATGG GCAGGAGGAT TCCATCTATC AGTCATTGGA GGCTGAGGAG
    AAGGAACCCC TGGATATTCC ATTGGAGAAG GACAGGACAC CAGAAGCTGT GCCAGAGGAG
    GTAGTAGAGC CAACAGTACC AGCAGTCCAG TCTCCCCAAG CTGCAGACAG TTACAGAGAA
    GAAGAACCTG GAATGTCCAG GGCCAAATCT AATTGGTTAA GAGCATTCAA CAAGGTGCGC
    CTGCAGCTGC AAGAGGCACG AGGTGAAGGG ACTGGGGAGA TGTCCAAATC CCTCTGGTTT
    AAGGATGGGC CAGCTGGTGG TCTTATAATT ATAGACAGTA TGCCAGACAT ACGAAAGAGG
    AAACCAATCC CGTTGGTCAG TGACCTGGCA ATGTCACTGG TTCAGTCACG GAAGGCTGGG
    ATTACTTCTG CTCTTGCAAC AAGCACCTTA AACAATGAAG AGCTAAAAAA CCATGTTTAC
    AAAAAGACCC TGCAAGCTTT AATATACCCA ATATCCAGCA CCACTCCCCA CAACTTTGAA
    GTTTGGACTG CCACAACTCC TACCTATTGT TATGAATGCG AAGGGCTTCT GTGGGGAATC
    GCTCGGCAGG GCATGCGTTG CACAGAGTGT GGTGTCAAGT GCCATGAGAA GTGCCAGGAT
    CTGCTGAATG CAGATTGTCT TCAAAGAGCT GCAGAGAAGA GCTCAAAACA TGGTGCAGAA
    GACCGGACTC AGAATATTAT TATGGTCTTG AAGGATCGTA TGAAGATCCG GGAGAGGAAC
    AAGCCAGAAA TATTTGAGCT GATCCAGGAA GTATTTGGGG TATTAAAGGC TACACACGCT
    CAGCAGATAA AGGCTGTGAA ACAGAGTGTG CTGGATGGTA CTTCCAAATG GTCAGCAAAG
    ATCAGCATAA CTGTTGTATG TGCACAGGGT CTACAGGCCA AAGATAAGAC AGGCTCCAGT
    GACCCATATG TTACTGTTCA AGTTGGGAAA ACCAAGAAAA GAACCAAAAC AATCTATGGA
    AATCTTAATC CTGTCTGGGA AGAGAATTTT CACTTTGAAT GCCACAACTC CTCAGATCGT
    ATTAAAGTAC GCGTGTGGGA TGAAGATGAT GATATCAAAT CTCGTGTGAA GCAGAGATTT
    AAAAGAGAAT CAGATGACTT CCTTGGACAA ACAATTATTG AAGTCAGAAC ACTTAGCGGA
    GAGATGGATG TGTGGTATAA CTTGGATAAG AGAACAGATA AATCCGCTGT CTCTGGGGCA
    ATCCGTCTTC ATATAAGTGT GGAAATAAAA GGAGAGGAAA AGGTGGCTCC CTATCACGTG
    CAATACACAT GTCTACATGA GAACTTATTC CATTACCTGA CGGATGTGCA GAATAATGGT
    GTGGTCAAGA TTCCAGATGC AAAAGGAGAC GACGCCTGGA AAGTTTATTT TGATGAGACA
    GCCCAGGAAA TTGTGGATGA ATTTGCAATG AGATATGGAG TGGAGTCCAT ATATCAGGCC
    ATGACGCATT TTGCCTGCCT TTCATCTAAA TATATGTGTC CAGGAGTACC AGCTGTAATG
    AGTACTCTTC TTGCCAACAT TAATGCTTAC TATGCGCACA CAACTGCATC CACTAATGTG
    TCTGCCTCCG ACCGATTCTC TGCCTCAAAC TTTGGGAAGG AGAGATTTGT AAAGCTGCTT
    GACCAGTTGC ACAATTCCTT ACGCATTGAC CTGTCCATGT ATCGGAACAA CTTCCCTGCT
    AGCAGCCCTG AAAGACTCCA AGATCTCAAG TCCACTGTAG ATTTACTAAC TAGCATAACA
    TTTTTCCGGA TGAAGGTACA AGAGCTTCAG AGCCCACCAC GGGCAAGCCA AGTTGTTAAG
    GACTGTGTGA AGGCATGCCT TAACTCAACC TACGAATATA TCTTTAACAA CTGCCATGAA
    CTTTATAGCC GAGAATATCA AAGTGACCCT GCTAAAAAAG GGGAGGTACC CCCAGAAGAA
    CAGGGGCCAA GTATAAAGAA CTTAGATTTT TGGTCCAAAC TAATCACATT GATTGTGTCT
    ATCATAGAGG AAGATAAGAA CTCATATACA CCTTGTCTAA ACCAGTTTCC TCAGGAGCTA
    AATGTTGGTA AGATTAGTGC TGAGGTCATG TGGAGTCTCT TTGCTCAAGA CATGAGATAT
    GCTATGGAAG AACACGACAA ACACCGCCTG TGTAAGAGTG CAGACTACAT GAACTTGCAT
    TTTAAGGTCA AGTGGTTGTA CAATGAATAT GTAACTGAGC TACCATCTTT TAAGAGCAAG
    GTTCCAGAAT ATCCCGCCTG GTTTGAGCCT TTTGTAATTC AGTGGCTGGA TGAAAATGAA
    GAGGTCTCCC GGGATTTTCT GCATGGAGCA CTTGAAAGAG ATAAGAAGGA TGGGTTCCAG
    CAGACCTCTG AACATGCCCT GTTCTCCTGC TCTGTGGTGG ATGTTTTCTC ACAACTTAAC
    CAGAGCTTTG AGATCATTAA GAAGTTGGAG TGTCCAGACC CACAAATTGT GGGGCATTAC
    ATGCGTAGAT TTGCCAAGAC GATCAGTAAT GTTCTGCTGC AGTATGCTGA AATTATTTCC
    AAAGATTTTG CATCTCATTG CTCCAAAGAG AAGAAAGAAG AAAAAGTGCC CTGTATTCTT
    ATGAACAACA TCCAGCAACT TCGAGTGCAA CTAGAAAAAA TGTTTGAAGC AATGGGAGGA
    AAGGAGCTGG ATGCAGAAAC AAGTGACATT CTTAAAGAGC TGCAGGTGAA ACTCAACAAT
    GTCCTAGATG AGCTAAGCAG AGTGTTTACC ACCAGTTTTC AGGCACAAAT TGAGGAGTGT
    GTGAAGCAGA TGGGTGACAT CCTGAGCCAG GTAAAAGGGA CCGGAAATGT ACCAGCCAGT
    AACTGCAGCA GTGTGGCTCA GGATGCAGAT AATGTATTGC AGCCTATCCT GGATCCTCTG
    GACAGCAACC TGACACTATT TGCCAAGATC TGTGAAAAGA CAGTCCTGAA GCGGGTACTA
    AAAGAACTCT GGAAGCTTGT GATGAATACA ATGGAGAAAA CGATTGTCCT TCCACCACTA
    ACAGACCAAA CAATGATAGG GACGCTGCTG AGAAAACATG GCAAGGGAAT AGAGAAGAGC
    AGGGTGAAGC TGCCGTACCA CGCAGATGGA ACCCACCTTA TCATTAATGC AGCCAAGGAA
    CTGGGCCAGT TATCCAAACT AAAGGATCAC ATGGTGCGAG AGGAAGCCAA GAGCCTCACC
    CCTAAGCAGT GTGCAGTGGT GGAGCTTGCA CTTGATACAA TTAAGCAATA TTTCCATGCT
    GGAGGTGTTG GTCTCAAGAA GACATTCCTG GAGAAGAGTC CAGACCTACA ATCTCTACGC
    TATGCACTTT CTCTTTACAC ACAAGCAACC GATACGCTAA TCAAAACCTT CGTCCAGACA
    CAGTCTGCTC AGGGATCTGG GGTGGATGAT TCTGTTGGGG AAGTTTCTAT CCATGTGGAG
    TTATTTACTC ACCCAGGAAC AGGCGAGCAC AAGATCAGCG TAAAGGTTGT AGCGGCCAAT
    GACCTTAAAT GGCAGACGTC AGGGATTTTC CGTCCCTTTA TTGAGGTAAA CATGATTGGT
    CCGAATCTGA GTGACAAAAA AAGGAAATTT ACCACCAAGT CCAAGAACAA CAGCTGGGCC
    CCAAAATATA ACGAGAGCTT CCAATTTACC CTGGGTAATG AGACTGGTCC AGAGAGTTAT
    GAGCTGCAAG TATGTGTGAA AGATTACTGT TTTGCTCGGG AAGACCGCAC AGTGGGAATG
    GCTGTCATGC AACTGCGAGA TCTGGCACAA AGGGGTAGCT GTGCCTGTTG GCTCCCTCTT
    GGCCGCCGCA TTCATATGGA TGAGACAGGA CTGACCATCT TGCGCATACT ATCACAAAGA
    AACAATGATG AGGTGGCCAA AGAGTTTGTC AAACTCAAAT CTGACACCCG CTCTCCAGAA
    GAGGGGAGCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa48433
    Clone ID Related Accession (Same CDS sequence) XM_031904667.1
    Accession Version XM_031904667.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5160bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein unc-13 homolog A isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCTTTGC TTTGCGTGGG AGTAAAAAAG GCCACGTTTG ATGGAGCACA AGAGAAGTTT 
    AATGCCTACG TAACCTTGAA GGTGCAAAAT GTGAAGAGCA CAACGATAGC GGTGCGGGGA
    AACCAGCCCT GCTGGGAACA GGATTTCATG TTTGAGATTA ATCGTTTAGA CCTTGGTCTA
    ACAGTGGAGG TCTGGAACAA AGGATTGATC TGGGACACCA TGGTGGGAAC AGTATGGATT
    CCTTTAAGGA CAATCCGACA ATCCAATGAG GAGGGTCCTG GGGAATGGCT GACACTTGAT
    TCACAGGTCA TAATGACAGA TAATGAGATT TGTGGTACCA AAGATCCAAC CTTCCACCGC
    ATCTTACTGG ATACCCGATT TGAACTTCCT CTAGATATAC CAGAAGAAGA AGCTCGATAC
    TGGGCAAAGA AACTTGAGCA ACTTAATGCA ATGAGGGACC AGGATGATTA TGTTTTTCAA
    GAAGAAATTC CGGAGAATCC TCTTCCCGTC CATGGATCTC AATGTTGTAA CTGGAATTAT
    TTCGGCTGGG GGGAGCAGCA CAATGAGGAT CCCGATAGTG CAGTGGATGA TCGGGATAGT
    GATTACCGCA GTGAAACCAG CAACAGTATC CCGCCCCCCT ACTACTCCAC CTCACAGCCT
    AATGCCTCTG TGCACCAGTA CCCTGTACGA CAGCAACACA GTTCTCATGG ATCTTACACT
    GATTCAGTGC ACAGCTATGA GATGGACTTC TCTGACCATC GTCCAATCAG CAGGCGTTAC
    GATAGCGCAG ACTCTGCCAC TAACGGGCGC AGTGATGTGG AGTCTGTCTC CGGTTCGAGC
    CGCCTCACCA GCCGGCAGCA CTCTCTGGAG AGCCGGCAGT CCTTAGACCT GTCCGATAGC
    CCCACTGGAA GTAGTCGATA TGCTTCATCA GGGGAGATTA GTCGTGGCTC CTCACAGCTC
    AGTGATGACT TTGACCCAGA TGATGACAGC TTGCAAGGTT CAGAAATAGA GGAAGAAAGG
    GATAGAGACT CTTATCACTC CTGTCATAGT TCTGTCAGCT GCCGCAAGGA CTCGCCAAAT
    TGGGAAGGAG AGGAGGAGCC TGAAGTTTAT AGTGAAGATG AGGAAGAATA TCCTGACAGT
    AGGGGGCAGC TCTCTGACCA GGAGCTGTAT GATGATGACC AGCTTGAGGA AGAAGAGAAA
    AAAGGAAGAT TAGCATATGG GCAGGAGGAT TCCATCTATC AGTCATTGGA GGCTGAGGAG
    AAGGAACCCC TGGATATTCC ATTGGAGAAG GACAGGACAC CAGAAGCTGT GCCAGAGGAG
    GTAGTAGAGC CAACAGTACC AGCAGTCCAG TCTCCCCAAG CTGCAGACAG TTACAGAGAA
    GAAGAACCTG GAATGTCCAG GGCCAAATCT AATTGGTTAA GAGCATTCAA CAAGGTGCGC
    CTGCAGCTGC AAGAGGCACG AGGTGAAGGG ACTGGGGAGA TGTCCAAATC CCTCTGGTTT
    AAGGATGGGC CAGCTGGTGG TCTTATAATT ATAGACAGTA TGCCAGACAT ACGAAAGAGG
    AAACCAATCC CGTTGGTCAG TGACCTGGCA ATGTCACTGG TTCAGTCACG GAAGGCTGGG
    ATTACTTCTG CTCTTGCAAC AAGCACCTTA AACAATGAAG AGCTAAAAAA CCATGTTTAC
    AAAAAGACCC TGCAAGCTTT AATATACCCA ATATCCAGCA CCACTCCCCA CAACTTTGAA
    GTTTGGACTG CCACAACTCC TACCTATTGT TATGAATGCG AAGGGCTTCT GTGGGGAATC
    GCTCGGCAGG GCATGCGTTG CACAGAGTGT GGTGTCAAGT GCCATGAGAA GTGCCAGGAT
    CTGCTGAATG CAGATTGTCT TCAAAGAGCT GCAGAGAAGA GCTCAAAACA TGGTGCAGAA
    GACCGGACTC AGAATATTAT TATGGTCTTG AAGGATCGTA TGAAGATCCG GGAGAGGAAC
    AAGCCAGAAA TATTTGAGCT GATCCAGGAA GTATTTGGGG TATTAAAGGC TACACACGCT
    CAGCAGATAA AGGCTGTGAA ACAGAGTGTG CTGGATGGTA CTTCCAAATG GTCAGCAAAG
    ATCAGCATAA CTGTTGTATG TGCACAGGGT CTACAGGCCA AAGATAAGAC AGGCTCCAGT
    GACCCATATG TTACTGTTCA AGTTGGGAAA ACCAAGAAAA GAACCAAAAC AATCTATGGA
    AATCTTAATC CTGTCTGGGA AGAGAATTTT CACTTTGAAT GCCACAACTC CTCAGATCGT
    ATTAAAGTAC GCGTGTGGGA TGAAGATGAT GATATCAAAT CTCGTGTGAA GCAGAGATTT
    AAAAGAGAAT CAGATGACTT CCTTGGACAA ACAATTATTG AAGTCAGAAC ACTTAGCGGA
    GAGATGGATG TGTGGTATAA CTTGGATAAG AGAACAGATA AATCCGCTGT CTCTGGGGCA
    ATCCGTCTTC ATATAAGTGT GGAAATAAAA GGAGAGGAAA AGGTGGCTCC CTATCACGTG
    CAATACACAT GTCTACATGA GAACTTATTC CATTACCTGA CGGATGTGCA GAATAATGGT
    GTGGTCAAGA TTCCAGATGC AAAAGGAGAC GACGCCTGGA AAGTTTATTT TGATGAGACA
    GCCCAGGAAA TTGTGGATGA ATTTGCAATG AGATATGGAG TGGAGTCCAT ATATCAGGCC
    ATGACGCATT TTGCCTGCCT TTCATCTAAA TATATGTGTC CAGGAGTACC AGCTGTAATG
    AGTACTCTTC TTGCCAACAT TAATGCTTAC TATGCGCACA CAACTGCATC CACTAATGTG
    TCTGCCTCCG ACCGATTCTC TGCCTCAAAC TTTGGGAAGG AGAGATTTGT AAAGCTGCTT
    GACCAGTTGC ACAATTCCTT ACGCATTGAC CTGTCCATGT ATCGGAACAA CTTCCCTGCT
    AGCAGCCCTG AAAGACTCCA AGATCTCAAG TCCACTGTAG ATTTACTAAC TAGCATAACA
    TTTTTCCGGA TGAAGGTACA AGAGCTTCAG AGCCCACCAC GGGCAAGCCA AGTTGTTAAG
    GACTGTGTGA AGGCATGCCT TAACTCAACC TACGAATATA TCTTTAACAA CTGCCATGAA
    CTTTATAGCC GAGAATATCA AAGTGACCCT GCTAAAAAAG GGGAGGTACC CCCAGAAGAA
    CAGGGGCCAA GTATAAAGAA CTTAGATTTT TGGTCCAAAC TAATCACATT GATTGTGTCT
    ATCATAGAGG AAGATAAGAA CTCATATACA CCTTGTCTAA ACCAGTTTCC TCAGGAGCTA
    AATGTTGGTA AGATTAGTGC TGAGGTCATG TGGAGTCTCT TTGCTCAAGA CATGAGATAT
    GCTATGGAAG AACACGACAA ACACCGCCTG TGTAAGAGTG CAGACTACAT GAACTTGCAT
    TTTAAGGTCA AGTGGTTGTA CAATGAATAT GTAACTGAGC TACCATCTTT TAAGAGCAAG
    GTTCCAGAAT ATCCCGCCTG GTTTGAGCCT TTTGTAATTC AGTGGCTGGA TGAAAATGAA
    GAGGTCTCCC GGGATTTTCT GCATGGAGCA CTTGAAAGAG ATAAGAAGGA TGGGTTCCAG
    CAGACCTCTG AACATGCCCT GTTCTCCTGC TCTGTGGTGG ATGTTTTCTC ACAACTTAAC
    CAGAGCTTTG AGATCATTAA GAAGTTGGAG TGTCCAGACC CACAAATTGT GGGGCATTAC
    ATGCGTAGAT TTGCCAAGAC GATCAGTAAT GTTCTGCTGC AGTATGCTGA AATTATTTCC
    AAAGATTTTG CATCTCATTG CTCCAAAGAG AAGAAAGAAG AAAAAGTGCC CTGTATTCTT
    ATGAACAACA TCCAGCAACT TCGAGTGCAA CTAGAAAAAA TGTTTGAAGC AATGGGAGGA
    AAGGAGCTGG ATGCAGAAAC AAGTGACATT CTTAAAGAGC TGCAGGTGAA ACTCAACAAT
    GTCCTAGATG AGCTAAGCAG AGTGTTTACC ACCAGTTTTC AGGCACAAAT TGAGGAGTGT
    GTGAAGCAGA TGGGTGACAT CCTGAGCCAG GTAAAAGGGA CCGGAAATGT ACCAGCCAGT
    AACTGCAGCA GTGTGGCTCA GGATGCAGAT AATGTATTGC AGCCTATCCT GGATCCTCTG
    GACAGCAACC TGACACTATT TGCCAAGATC TGTGAAAAGA CAGTCCTGAA GCGGGTACTA
    AAAGAACTCT GGAAGCTTGT GATGAATACA ATGGAGAAAA CGATTGTCCT TCCACCACTA
    ACAGACCAAA CAATGATAGG AACCCACCTT ATCATTAATG CAGCCAAGGA ACTGGGCCAG
    TTATCCAAAC TAAAGGATCA CATGGTGCGA GAGGAAGCCA AGAGCCTCAC CCCTAAGCAG
    TGTGCAGTGG TGGAGCTTGC ACTTGATACA ATTAAGCAAT ATTTCCATGC TGGAGGTGTT
    GGTCTCAAGA AGACATTCCT GGAGAAGAGT CCAGACCTAC AATCTCTACG CTATGCACTT
    TCTCTTTACA CACAAGCAAC CGATACGCTA ATCAAAACCT TCGTCCAGAC ACAGTCTGCT
    CAGGTTCATG GTGGTAAAGG TATTAGGTTT ACACTTAATG AAGAGACTTA TCCAGAAAAG
    GGATCTGGGG TGGATGATTC TGTTGGGGAA GTTTCTATCC ATGTGGAGTT ATTTACTCAC
    CCAGGAACAG GCGAGCACAA GATCAGCGTA AAGGTTGTAG CGGCCAATGA CCTTAAATGG
    CAGACGTCAG GGATTTTCCG TCCCTTTATT GAGGTAAACA TGATTGGTCC GAATCTGAGT
    GACAAAAAAA GGAAATTTAC CACCAAGTCC AAGAACAACA GCTGGGCCCC AAAATATAAC
    GAGAGCTTCC AATTTACCCT GGGTAATGAG ACTGGTCCAG AGAGTTATGA GCTGCAAGTA
    TGTGTGAAAG ATTACTGTTT TGCTCGGGAA GACCGCACAG TGGGAATGGC TGTCATGCAA
    CTGCGAGATC TGGCACAAAG GGGTAGCTGT GCCTGTTGGC TCCCTCTTGG CCGCCGCATT
    CATATGGATG AGACAGGACT GACCATCTTG CGCATACTAT CACAAAGAAA CAATGATGAG
    GTGGCCAAAG AGTTTGTCAA ACTCAAATCT GACACCCGCT CTCCAGAAGA GGGGAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031760527.1
    CDS188..5347
    Translation

    Target ORF information:

    RefSeq Version XM_031904667.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis unc-13 homolog A (unc13a), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031904667.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCTTTGC TTTGCGTGGG AGTAAAAAAG GCCACGTTTG ATGGAGCACA AGAGAAGTTT 
    AATGCCTACG TAACCTTGAA GGTGCAAAAT GTGAAGAGCA CAACGATAGC GGTGCGGGGA
    AACCAGCCCT GCTGGGAACA GGATTTCATG TTTGAGATTA ATCGTTTAGA CCTTGGTCTA
    ACAGTGGAGG TCTGGAACAA AGGATTGATC TGGGACACCA TGGTGGGAAC AGTATGGATT
    CCTTTAAGGA CAATCCGACA ATCCAATGAG GAGGGTCCTG GGGAATGGCT GACACTTGAT
    TCACAGGTCA TAATGACAGA TAATGAGATT TGTGGTACCA AAGATCCAAC CTTCCACCGC
    ATCTTACTGG ATACCCGATT TGAACTTCCT CTAGATATAC CAGAAGAAGA AGCTCGATAC
    TGGGCAAAGA AACTTGAGCA ACTTAATGCA ATGAGGGACC AGGATGATTA TGTTTTTCAA
    GAAGAAATTC CGGAGAATCC TCTTCCCGTC CATGGATCTC AATGTTGTAA CTGGAATTAT
    TTCGGCTGGG GGGAGCAGCA CAATGAGGAT CCCGATAGTG CAGTGGATGA TCGGGATAGT
    GATTACCGCA GTGAAACCAG CAACAGTATC CCGCCCCCCT ACTACTCCAC CTCACAGCCT
    AATGCCTCTG TGCACCAGTA CCCTGTACGA CAGCAACACA GTTCTCATGG ATCTTACACT
    GATTCAGTGC ACAGCTATGA GATGGACTTC TCTGACCATC GTCCAATCAG CAGGCGTTAC
    GATAGCGCAG ACTCTGCCAC TAACGGGCGC AGTGATGTGG AGTCTGTCTC CGGTTCGAGC
    CGCCTCACCA GCCGGCAGCA CTCTCTGGAG AGCCGGCAGT CCTTAGACCT GTCCGATAGC
    CCCACTGGAA GTAGTCGATA TGCTTCATCA GGGGAGATTA GTCGTGGCTC CTCACAGCTC
    AGTGATGACT TTGACCCAGA TGATGACAGC TTGCAAGGTT CAGAAATAGA GGAAGAAAGG
    GATAGAGACT CTTATCACTC CTGTCATAGT TCTGTCAGCT GCCGCAAGGA CTCGCCAAAT
    TGGGAAGGAG AGGAGGAGCC TGAAGTTTAT AGTGAAGATG AGGAAGAATA TCCTGACAGT
    AGGGGGCAGC TCTCTGACCA GGAGCTGTAT GATGATGACC AGCTTGAGGA AGAAGAGAAA
    AAAGGAAGAT TAGCATATGG GCAGGAGGAT TCCATCTATC AGTCATTGGA GGCTGAGGAG
    AAGGAACCCC TGGATATTCC ATTGGAGAAG GACAGGACAC CAGAAGCTGT GCCAGAGGAG
    GTAGTAGAGC CAACAGTACC AGCAGTCCAG TCTCCCCAAG CTGCAGACAG TTACAGAGAA
    GAAGAACCTG GAATGTCCAG GGCCAAATCT AATTGGTTAA GAGCATTCAA CAAGGTGCGC
    CTGCAGCTGC AAGAGGCACG AGGTGAAGGG ACTGGGGAGA TGTCCAAATC CCTCTGGTTT
    AAGGATGGGC CAGCTGGTGG TCTTATAATT ATAGACAGTA TGCCAGACAT ACGAAAGAGG
    AAACCAATCC CGTTGGTCAG TGACCTGGCA ATGTCACTGG TTCAGTCACG GAAGGCTGGG
    ATTACTTCTG CTCTTGCAAC AAGCACCTTA AACAATGAAG AGCTAAAAAA CCATGTTTAC
    AAAAAGACCC TGCAAGCTTT AATATACCCA ATATCCAGCA CCACTCCCCA CAACTTTGAA
    GTTTGGACTG CCACAACTCC TACCTATTGT TATGAATGCG AAGGGCTTCT GTGGGGAATC
    GCTCGGCAGG GCATGCGTTG CACAGAGTGT GGTGTCAAGT GCCATGAGAA GTGCCAGGAT
    CTGCTGAATG CAGATTGTCT TCAAAGAGCT GCAGAGAAGA GCTCAAAACA TGGTGCAGAA
    GACCGGACTC AGAATATTAT TATGGTCTTG AAGGATCGTA TGAAGATCCG GGAGAGGAAC
    AAGCCAGAAA TATTTGAGCT GATCCAGGAA GTATTTGGGG TATTAAAGGC TACACACGCT
    CAGCAGATAA AGGCTGTGAA ACAGAGTGTG CTGGATGGTA CTTCCAAATG GTCAGCAAAG
    ATCAGCATAA CTGTTGTATG TGCACAGGGT CTACAGGCCA AAGATAAGAC AGGCTCCAGT
    GACCCATATG TTACTGTTCA AGTTGGGAAA ACCAAGAAAA GAACCAAAAC AATCTATGGA
    AATCTTAATC CTGTCTGGGA AGAGAATTTT CACTTTGAAT GCCACAACTC CTCAGATCGT
    ATTAAAGTAC GCGTGTGGGA TGAAGATGAT GATATCAAAT CTCGTGTGAA GCAGAGATTT
    AAAAGAGAAT CAGATGACTT CCTTGGACAA ACAATTATTG AAGTCAGAAC ACTTAGCGGA
    GAGATGGATG TGTGGTATAA CTTGGATAAG AGAACAGATA AATCCGCTGT CTCTGGGGCA
    ATCCGTCTTC ATATAAGTGT GGAAATAAAA GGAGAGGAAA AGGTGGCTCC CTATCACGTG
    CAATACACAT GTCTACATGA GAACTTATTC CATTACCTGA CGGATGTGCA GAATAATGGT
    GTGGTCAAGA TTCCAGATGC AAAAGGAGAC GACGCCTGGA AAGTTTATTT TGATGAGACA
    GCCCAGGAAA TTGTGGATGA ATTTGCAATG AGATATGGAG TGGAGTCCAT ATATCAGGCC
    ATGACGCATT TTGCCTGCCT TTCATCTAAA TATATGTGTC CAGGAGTACC AGCTGTAATG
    AGTACTCTTC TTGCCAACAT TAATGCTTAC TATGCGCACA CAACTGCATC CACTAATGTG
    TCTGCCTCCG ACCGATTCTC TGCCTCAAAC TTTGGGAAGG AGAGATTTGT AAAGCTGCTT
    GACCAGTTGC ACAATTCCTT ACGCATTGAC CTGTCCATGT ATCGGAACAA CTTCCCTGCT
    AGCAGCCCTG AAAGACTCCA AGATCTCAAG TCCACTGTAG ATTTACTAAC TAGCATAACA
    TTTTTCCGGA TGAAGGTACA AGAGCTTCAG AGCCCACCAC GGGCAAGCCA AGTTGTTAAG
    GACTGTGTGA AGGCATGCCT TAACTCAACC TACGAATATA TCTTTAACAA CTGCCATGAA
    CTTTATAGCC GAGAATATCA AAGTGACCCT GCTAAAAAAG GGGAGGTACC CCCAGAAGAA
    CAGGGGCCAA GTATAAAGAA CTTAGATTTT TGGTCCAAAC TAATCACATT GATTGTGTCT
    ATCATAGAGG AAGATAAGAA CTCATATACA CCTTGTCTAA ACCAGTTTCC TCAGGAGCTA
    AATGTTGGTA AGATTAGTGC TGAGGTCATG TGGAGTCTCT TTGCTCAAGA CATGAGATAT
    GCTATGGAAG AACACGACAA ACACCGCCTG TGTAAGAGTG CAGACTACAT GAACTTGCAT
    TTTAAGGTCA AGTGGTTGTA CAATGAATAT GTAACTGAGC TACCATCTTT TAAGAGCAAG
    GTTCCAGAAT ATCCCGCCTG GTTTGAGCCT TTTGTAATTC AGTGGCTGGA TGAAAATGAA
    GAGGTCTCCC GGGATTTTCT GCATGGAGCA CTTGAAAGAG ATAAGAAGGA TGGGTTCCAG
    CAGACCTCTG AACATGCCCT GTTCTCCTGC TCTGTGGTGG ATGTTTTCTC ACAACTTAAC
    CAGAGCTTTG AGATCATTAA GAAGTTGGAG TGTCCAGACC CACAAATTGT GGGGCATTAC
    ATGCGTAGAT TTGCCAAGAC GATCAGTAAT GTTCTGCTGC AGTATGCTGA AATTATTTCC
    AAAGATTTTG CATCTCATTG CTCCAAAGAG AAGAAAGAAG AAAAAGTGCC CTGTATTCTT
    ATGAACAACA TCCAGCAACT TCGAGTGCAA CTAGAAAAAA TGTTTGAAGC AATGGGAGGA
    AAGGAGCTGG ATGCAGAAAC AAGTGACATT CTTAAAGAGC TGCAGGTGAA ACTCAACAAT
    GTCCTAGATG AGCTAAGCAG AGTGTTTACC ACCAGTTTTC AGGCACAAAT TGAGGAGTGT
    GTGAAGCAGA TGGGTGACAT CCTGAGCCAG GTAAAAGGGA CCGGAAATGT ACCAGCCAGT
    AACTGCAGCA GTGTGGCTCA GGATGCAGAT AATGTATTGC AGCCTATCCT GGATCCTCTG
    GACAGCAACC TGACACTATT TGCCAAGATC TGTGAAAAGA CAGTCCTGAA GCGGGTACTA
    AAAGAACTCT GGAAGCTTGT GATGAATACA ATGGAGAAAA CGATTGTCCT TCCACCACTA
    ACAGACCAAA CAATGATAGG AACCCACCTT ATCATTAATG CAGCCAAGGA ACTGGGCCAG
    TTATCCAAAC TAAAGGATCA CATGGTGCGA GAGGAAGCCA AGAGCCTCAC CCCTAAGCAG
    TGTGCAGTGG TGGAGCTTGC ACTTGATACA ATTAAGCAAT ATTTCCATGC TGGAGGTGTT
    GGTCTCAAGA AGACATTCCT GGAGAAGAGT CCAGACCTAC AATCTCTACG CTATGCACTT
    TCTCTTTACA CACAAGCAAC CGATACGCTA ATCAAAACCT TCGTCCAGAC ACAGTCTGCT
    CAGGTTCATG GTGGTAAAGG TATTAGGTTT ACACTTAATG AAGAGACTTA TCCAGAAAAG
    GGATCTGGGG TGGATGATTC TGTTGGGGAA GTTTCTATCC ATGTGGAGTT ATTTACTCAC
    CCAGGAACAG GCGAGCACAA GATCAGCGTA AAGGTTGTAG CGGCCAATGA CCTTAAATGG
    CAGACGTCAG GGATTTTCCG TCCCTTTATT GAGGTAAACA TGATTGGTCC GAATCTGAGT
    GACAAAAAAA GGAAATTTAC CACCAAGTCC AAGAACAACA GCTGGGCCCC AAAATATAAC
    GAGAGCTTCC AATTTACCCT GGGTAATGAG ACTGGTCCAG AGAGTTATGA GCTGCAAGTA
    TGTGTGAAAG ATTACTGTTT TGCTCGGGAA GACCGCACAG TGGGAATGGC TGTCATGCAA
    CTGCGAGATC TGGCACAAAG GGGTAGCTGT GCCTGTTGGC TCCCTCTTGG CCGCCGCATT
    CATATGGATG AGACAGGACT GACCATCTTG CGCATACTAT CACAAAGAAA CAATGATGAG
    GTGGCCAAAG AGTTTGTCAA ACTCAAATCT GACACCCGCT CTCCAGAAGA GGGGAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa48434
    Clone ID Related Accession (Same CDS sequence) XM_031904668.1
    Accession Version XM_031904668.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5154bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein unc-13 homolog A isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCTTTGC TTTGCGTGGG AGTAAAAAAG GCCACGTTTG ATGGAGCACA AGAGAAGTTT 
    AATGCCTACG TAACCTTGAA GGTGCAAAAT GTGAAGAGCA CAACGATAGC GGTGCGGGGA
    AACCAGCCCT GCTGGGAACA GGATTTCATG TTTGAGATTA ATCGTTTAGA CCTTGGTCTA
    ACAGTGGAGG TCTGGAACAA AGGATTGATC TGGGACACCA TGGTGGGAAC AGTATGGATT
    CCTTTAAGGA CAATCCGACA ATCCAATGAG GAGGGTCCTG GGGAATGGCT GACACTTGAT
    TCACAGGTCA TAATGACAGA TAATGAGATT TGTGGTACCA AAGATCCAAC CTTCCACCGC
    ATCTTACTGG ATACCCGATT TGAACTTCCT CTAGATATAC CAGAAGAAGA AGCTCGATAC
    TGGGCAAAGA AACTTGAGCA ACTTAATGCA ATGAGGGACC AGGATGATTA TGTTTTTCAA
    GAAGAAATTC CGGAGAATCC TCTTCCCGTC CATGGATCTC AATGTTGTAA CTGGAATTAT
    TTCGGCTGGG GGGAGCAGCA CAATGAGGAT CCCGATAGTG CAGTGGATGA TCGGGATAGT
    GATTACCGCA GTGAAACCAG CAACAGTATC CCGCCCCCCT ACTACTCCAC CTCACAGCCT
    AATGCCTCTG TGCACCAGTA CCCTGTACGA CAGCAACACA GTTCTCATGG ATCTTACACT
    GATTCAGTGC ACAGCTATGA GATGGACTTC TCTGACCATC GTCCAATCAG CAGGCGTTAC
    GATAGCGCAG ACTCTGCCAC TAACGGGCGC AGTGATGTGG AGTCTGTCTC CGGTTCGAGC
    CGCCTCACCA GCCGGCAGCA CTCTCTGGAG AGCCGGCAGT CCTTAGACCT GTCCGATAGC
    CCCACTGGAA GTAGTCGATA TGCTTCATCA GGGGAGATTA GTCGTGGCTC CTCACAGCTC
    AGTGATGACT TTGACCCAGA TGATGACAGC TTGCAAGGTT CAGAAATAGA GGAAGAAAGG
    GATAGAGACT CTTATCACTC CTGTCATAGT TCTGTCAGCT GCCGCAAGGA CTCGCCAAAT
    TGGGAAGGAG AGGAGGAGCC TGAAGTTTAT AGTGAAGATG AGGAAGAATA TCCTGACAGT
    AGGGGGCAGC TCTCTGACCA GGAGCTGTAT GATGATGACC AGCTTGAGGA AGAAGAGAAA
    AAAGGAAGAT TAGCATATGG GCAGGAGGAT TCCATCTATC AGTCATTGGA GGCTGAGGAG
    AAGGAACCCC TGGATATTCC ATTGGAGAAG GACAGGACAC CAGAAGCTGT GCCAGAGGAG
    GTAGTAGAGC CAACAGTACC AGCAGTCCAG TCTCCCCAAG CTGCAGACAG TTACAGAGAA
    GAAGAACCTG GAATGTCCAG GGCCAAATCT AATTGGTTAA GAGCATTCAA CAAGGTGCGC
    CTGCAGCTGC AAGAGGCACG AGGTGAAGGG ACTGGGGAGA TGTCCAAATC CCTCTGGTTT
    AAGGATGGGC CAGCTGGTGG TCTTATAATT ATAGACAGTA TGCCAGACAT ACGAAAGAGG
    AAACCAATCC CGTTGGTCAG TGACCTGGCA ATGTCACTGG TTCAGTCACG GAAGGCTGGG
    ATTACTTCTG CTCTTGCAAC AAGCACCTTA AACAATGAAG AGCTAAAAAA CCATGTTTAC
    AAAAAGACCC TGCAAGCTTT AATATACCCA ATATCCAGCA CCACTCCCCA CAACTTTGAA
    GTTTGGACTG CCACAACTCC TACCTATTGT TATGAATGCG AAGGGCTTCT GTGGGGAATC
    GCTCGGCAGG GCATGCGTTG CACAGAGTGT GGTGTCAAGT GCCATGAGAA GTGCCAGGAT
    CTGCTGAATG CAGATTGTCT TCAAAGAGCT GCAGAGAAGA GCTCAAAACA TGGTGCAGAA
    GACCGGACTC AGAATATTAT TATGGTCTTG AAGGATCGTA TGAAGATCCG GGAGAGGAAC
    AAGCCAGAAA TATTTGAGCT GATCCAGGAA GTATTTGGGG TATTAAAGGC TACACACGCT
    CAGCAGATAA AGGCTGTGAA ACAGAGTGTG CTGGATGGTA CTTCCAAATG GTCAGCAAAG
    ATCAGCATAA CTGTTGTATG TGCACAGGGT CTACAGGCCA AAGATAAGAC AGGCTCCAGT
    GACCCATATG TTACTGTTCA AGTTGGGAAA ACCAAGAAAA GAACCAAAAC AATCTATGGA
    AATCTTAATC CTGTCTGGGA AGAGAATTTT CACTTTGAAT GCCACAACTC CTCAGATCGT
    ATTAAAGTAC GCGTGTGGGA TGAAGATGAT GATATCAAAT CTCGTGTGAA GCAGAGATTT
    AAAAGAGAAT CAGATGACTT CCTTGGACAA ACAATTATTG AAGTCAGAAC ACTTAGCGGA
    GAGATGGATG TGTGGTATAA CTTGGATAAG AGAACAGATA AATCCGCTGT CTCTGGGGCA
    ATCCGTCTTC ATATAAGTGT GGAAATAAAA GGAGAGGAAA AGGTGGCTCC CTATCACGTG
    CAATACACAT GTCTACATGA GAACTTATTC CATTACCTGA CGGATGTGCA GAATAATGGT
    GTGGTCAAGA TTCCAGATGC AAAAGGAGAC GACGCCTGGA AAGTTTATTT TGATGAGACA
    GCCCAGGAAA TTGTGGATGA ATTTGCAATG AGATATGGAG TGGAGTCCAT ATATCAGGCC
    ATGACGCATT TTGCCTGCCT TTCATCTAAA TATATGTGTC CAGGAGTACC AGCTGTAATG
    AGTACTCTTC TTGCCAACAT TAATGCTTAC TATGCGCACA CAACTGCATC CACTAATGTG
    TCTGCCTCCG ACCGATTCTC TGCCTCAAAC TTTGGGAAGG AGAGATTTGT AAAGCTGCTT
    GACCAGTTGC ACAATTCCTT ACGCATTGAC CTGTCCATGT ATCGGAACAA CTTCCCTGCT
    AGCAGCCCTG AAAGACTCCA AGATCTCAAG TCCACTGTAG ATTTACTAAC TAGCATAACA
    TTTTTCCGGA TGAAGGTACA AGAGCTTCAG AGCCCACCAC GGGCAAGCCA AGTTGTTAAG
    GACTGTGTGA AGGCATGCCT TAACTCAACC TACGAATATA TCTTTAACAA CTGCCATGAA
    CTTTATAGCC GAGAATATCA AAGTGACCCT GCTAAAAAAG GGGAGGTACC CCCAGAAGAA
    CAGGGGCCAA GTATAAAGAA CTTAGATTTT TGGTCCAAAC TAATCACATT GATTGTGTCT
    ATCATAGAGG AAGATAAGAA CTCATATACA CCTTGTCTAA ACCAGTTTCC TCAGGAGCTA
    AATGTTGGTA AGATTAGTGC TGAGGTCATG TGGAGTCTCT TTGCTCAAGA CATGAGATAT
    GCTATGGAAG AACACGACAA ACACCGCCTG TGTAAGAGTG CAGACTACAT GAACTTGCAT
    TTTAAGGTCA AGTGGTTGTA CAATGAATAT GTAACTGAGC TACCATCTTT TAAGAGCAAG
    GTTCCAGAAT ATCCCGCCTG GTTTGAGCCT TTTGTAATTC AGTGGCTGGA TGAAAATGAA
    GAGGTCTCCC GGGATTTTCT GCATGGAGCA CTTGAAAGAG ATAAGAAGGA TGGGTTCCAG
    CAGACCTCTG AACATGCCCT GTTCTCCTGC TCTGTGGTGG ATGTTTTCTC ACAACTTAAC
    CAGAGCTTTG AGATCATTAA GAAGTTGGAG TGTCCAGACC CACAAATTGT GGGGCATTAC
    ATGCGTAGAT TTGCCAAGAC GATCAGTAAT GTTCTGCTGC AGTATGCTGA AATTATTTCC
    AAAGATTTTG CATCTCATTG CTCCAAAGAG AAGAAAGAAG AAAAAGTGCC CTGTATTCTT
    ATGAACAACA TCCAGCAACT TCGAGTGCAA CTAGAAAAAA TGTTTGAAGC AATGGGAGGA
    AAGGAGCTGG ATGCAGAAAC AAGTGACATT CTTAAAGAGC TGCAGGTGAA ACTCAACAAT
    GTCCTAGATG AGCTAAGCAG AGTGTTTACC ACCAGTTTTC AGGCACAAAT TGAGGAGTGT
    GTGAAGCAGA TGGGTGACAT CCTGAGCCAG GTAAAAGGGA CCGGAAATGT ACCAGCCAGT
    AACTGCAGCA GTGTGGCTCA GGATGCAGAT AATGTATTGC AGCCTATCCT GGATCCTCTG
    GACAGCAACC TGACACTATT TGCCAAGATC TGTGAAAAGA CAGTCCTGAA GCGGGTACTA
    AAAGAACTCT GGAAGCTTGT GATGAATACA ATGGAGAAAA CGATTGTCCT TCCACCACTA
    ACAGACCAAA CAGGAACCCA CCTTATCATT AATGCAGCCA AGGAACTGGG CCAGTTATCC
    AAACTAAAGG ATCACATGGT GCGAGAGGAA GCCAAGAGCC TCACCCCTAA GCAGTGTGCA
    GTGGTGGAGC TTGCACTTGA TACAATTAAG CAATATTTCC ATGCTGGAGG TGTTGGTCTC
    AAGAAGACAT TCCTGGAGAA GAGTCCAGAC CTACAATCTC TACGCTATGC ACTTTCTCTT
    TACACACAAG CAACCGATAC GCTAATCAAA ACCTTCGTCC AGACACAGTC TGCTCAGGTT
    CATGGTGGTA AAGGTATTAG GTTTACACTT AATGAAGAGA CTTATCCAGA AAAGGGATCT
    GGGGTGGATG ATTCTGTTGG GGAAGTTTCT ATCCATGTGG AGTTATTTAC TCACCCAGGA
    ACAGGCGAGC ACAAGATCAG CGTAAAGGTT GTAGCGGCCA ATGACCTTAA ATGGCAGACG
    TCAGGGATTT TCCGTCCCTT TATTGAGGTA AACATGATTG GTCCGAATCT GAGTGACAAA
    AAAAGGAAAT TTACCACCAA GTCCAAGAAC AACAGCTGGG CCCCAAAATA TAACGAGAGC
    TTCCAATTTA CCCTGGGTAA TGAGACTGGT CCAGAGAGTT ATGAGCTGCA AGTATGTGTG
    AAAGATTACT GTTTTGCTCG GGAAGACCGC ACAGTGGGAA TGGCTGTCAT GCAACTGCGA
    GATCTGGCAC AAAGGGGTAG CTGTGCCTGT TGGCTCCCTC TTGGCCGCCG CATTCATATG
    GATGAGACAG GACTGACCAT CTTGCGCATA CTATCACAAA GAAACAATGA TGAGGTGGCC
    AAAGAGTTTG TCAAACTCAA ATCTGACACC CGCTCTCCAG AAGAGGGGAG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031760528.1
    CDS188..5341
    Translation

    Target ORF information:

    RefSeq Version XM_031904668.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis unc-13 homolog A (unc13a), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031904668.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCTTTGC TTTGCGTGGG AGTAAAAAAG GCCACGTTTG ATGGAGCACA AGAGAAGTTT 
    AATGCCTACG TAACCTTGAA GGTGCAAAAT GTGAAGAGCA CAACGATAGC GGTGCGGGGA
    AACCAGCCCT GCTGGGAACA GGATTTCATG TTTGAGATTA ATCGTTTAGA CCTTGGTCTA
    ACAGTGGAGG TCTGGAACAA AGGATTGATC TGGGACACCA TGGTGGGAAC AGTATGGATT
    CCTTTAAGGA CAATCCGACA ATCCAATGAG GAGGGTCCTG GGGAATGGCT GACACTTGAT
    TCACAGGTCA TAATGACAGA TAATGAGATT TGTGGTACCA AAGATCCAAC CTTCCACCGC
    ATCTTACTGG ATACCCGATT TGAACTTCCT CTAGATATAC CAGAAGAAGA AGCTCGATAC
    TGGGCAAAGA AACTTGAGCA ACTTAATGCA ATGAGGGACC AGGATGATTA TGTTTTTCAA
    GAAGAAATTC CGGAGAATCC TCTTCCCGTC CATGGATCTC AATGTTGTAA CTGGAATTAT
    TTCGGCTGGG GGGAGCAGCA CAATGAGGAT CCCGATAGTG CAGTGGATGA TCGGGATAGT
    GATTACCGCA GTGAAACCAG CAACAGTATC CCGCCCCCCT ACTACTCCAC CTCACAGCCT
    AATGCCTCTG TGCACCAGTA CCCTGTACGA CAGCAACACA GTTCTCATGG ATCTTACACT
    GATTCAGTGC ACAGCTATGA GATGGACTTC TCTGACCATC GTCCAATCAG CAGGCGTTAC
    GATAGCGCAG ACTCTGCCAC TAACGGGCGC AGTGATGTGG AGTCTGTCTC CGGTTCGAGC
    CGCCTCACCA GCCGGCAGCA CTCTCTGGAG AGCCGGCAGT CCTTAGACCT GTCCGATAGC
    CCCACTGGAA GTAGTCGATA TGCTTCATCA GGGGAGATTA GTCGTGGCTC CTCACAGCTC
    AGTGATGACT TTGACCCAGA TGATGACAGC TTGCAAGGTT CAGAAATAGA GGAAGAAAGG
    GATAGAGACT CTTATCACTC CTGTCATAGT TCTGTCAGCT GCCGCAAGGA CTCGCCAAAT
    TGGGAAGGAG AGGAGGAGCC TGAAGTTTAT AGTGAAGATG AGGAAGAATA TCCTGACAGT
    AGGGGGCAGC TCTCTGACCA GGAGCTGTAT GATGATGACC AGCTTGAGGA AGAAGAGAAA
    AAAGGAAGAT TAGCATATGG GCAGGAGGAT TCCATCTATC AGTCATTGGA GGCTGAGGAG
    AAGGAACCCC TGGATATTCC ATTGGAGAAG GACAGGACAC CAGAAGCTGT GCCAGAGGAG
    GTAGTAGAGC CAACAGTACC AGCAGTCCAG TCTCCCCAAG CTGCAGACAG TTACAGAGAA
    GAAGAACCTG GAATGTCCAG GGCCAAATCT AATTGGTTAA GAGCATTCAA CAAGGTGCGC
    CTGCAGCTGC AAGAGGCACG AGGTGAAGGG ACTGGGGAGA TGTCCAAATC CCTCTGGTTT
    AAGGATGGGC CAGCTGGTGG TCTTATAATT ATAGACAGTA TGCCAGACAT ACGAAAGAGG
    AAACCAATCC CGTTGGTCAG TGACCTGGCA ATGTCACTGG TTCAGTCACG GAAGGCTGGG
    ATTACTTCTG CTCTTGCAAC AAGCACCTTA AACAATGAAG AGCTAAAAAA CCATGTTTAC
    AAAAAGACCC TGCAAGCTTT AATATACCCA ATATCCAGCA CCACTCCCCA CAACTTTGAA
    GTTTGGACTG CCACAACTCC TACCTATTGT TATGAATGCG AAGGGCTTCT GTGGGGAATC
    GCTCGGCAGG GCATGCGTTG CACAGAGTGT GGTGTCAAGT GCCATGAGAA GTGCCAGGAT
    CTGCTGAATG CAGATTGTCT TCAAAGAGCT GCAGAGAAGA GCTCAAAACA TGGTGCAGAA
    GACCGGACTC AGAATATTAT TATGGTCTTG AAGGATCGTA TGAAGATCCG GGAGAGGAAC
    AAGCCAGAAA TATTTGAGCT GATCCAGGAA GTATTTGGGG TATTAAAGGC TACACACGCT
    CAGCAGATAA AGGCTGTGAA ACAGAGTGTG CTGGATGGTA CTTCCAAATG GTCAGCAAAG
    ATCAGCATAA CTGTTGTATG TGCACAGGGT CTACAGGCCA AAGATAAGAC AGGCTCCAGT
    GACCCATATG TTACTGTTCA AGTTGGGAAA ACCAAGAAAA GAACCAAAAC AATCTATGGA
    AATCTTAATC CTGTCTGGGA AGAGAATTTT CACTTTGAAT GCCACAACTC CTCAGATCGT
    ATTAAAGTAC GCGTGTGGGA TGAAGATGAT GATATCAAAT CTCGTGTGAA GCAGAGATTT
    AAAAGAGAAT CAGATGACTT CCTTGGACAA ACAATTATTG AAGTCAGAAC ACTTAGCGGA
    GAGATGGATG TGTGGTATAA CTTGGATAAG AGAACAGATA AATCCGCTGT CTCTGGGGCA
    ATCCGTCTTC ATATAAGTGT GGAAATAAAA GGAGAGGAAA AGGTGGCTCC CTATCACGTG
    CAATACACAT GTCTACATGA GAACTTATTC CATTACCTGA CGGATGTGCA GAATAATGGT
    GTGGTCAAGA TTCCAGATGC AAAAGGAGAC GACGCCTGGA AAGTTTATTT TGATGAGACA
    GCCCAGGAAA TTGTGGATGA ATTTGCAATG AGATATGGAG TGGAGTCCAT ATATCAGGCC
    ATGACGCATT TTGCCTGCCT TTCATCTAAA TATATGTGTC CAGGAGTACC AGCTGTAATG
    AGTACTCTTC TTGCCAACAT TAATGCTTAC TATGCGCACA CAACTGCATC CACTAATGTG
    TCTGCCTCCG ACCGATTCTC TGCCTCAAAC TTTGGGAAGG AGAGATTTGT AAAGCTGCTT
    GACCAGTTGC ACAATTCCTT ACGCATTGAC CTGTCCATGT ATCGGAACAA CTTCCCTGCT
    AGCAGCCCTG AAAGACTCCA AGATCTCAAG TCCACTGTAG ATTTACTAAC TAGCATAACA
    TTTTTCCGGA TGAAGGTACA AGAGCTTCAG AGCCCACCAC GGGCAAGCCA AGTTGTTAAG
    GACTGTGTGA AGGCATGCCT TAACTCAACC TACGAATATA TCTTTAACAA CTGCCATGAA
    CTTTATAGCC GAGAATATCA AAGTGACCCT GCTAAAAAAG GGGAGGTACC CCCAGAAGAA
    CAGGGGCCAA GTATAAAGAA CTTAGATTTT TGGTCCAAAC TAATCACATT GATTGTGTCT
    ATCATAGAGG AAGATAAGAA CTCATATACA CCTTGTCTAA ACCAGTTTCC TCAGGAGCTA
    AATGTTGGTA AGATTAGTGC TGAGGTCATG TGGAGTCTCT TTGCTCAAGA CATGAGATAT
    GCTATGGAAG AACACGACAA ACACCGCCTG TGTAAGAGTG CAGACTACAT GAACTTGCAT
    TTTAAGGTCA AGTGGTTGTA CAATGAATAT GTAACTGAGC TACCATCTTT TAAGAGCAAG
    GTTCCAGAAT ATCCCGCCTG GTTTGAGCCT TTTGTAATTC AGTGGCTGGA TGAAAATGAA
    GAGGTCTCCC GGGATTTTCT GCATGGAGCA CTTGAAAGAG ATAAGAAGGA TGGGTTCCAG
    CAGACCTCTG AACATGCCCT GTTCTCCTGC TCTGTGGTGG ATGTTTTCTC ACAACTTAAC
    CAGAGCTTTG AGATCATTAA GAAGTTGGAG TGTCCAGACC CACAAATTGT GGGGCATTAC
    ATGCGTAGAT TTGCCAAGAC GATCAGTAAT GTTCTGCTGC AGTATGCTGA AATTATTTCC
    AAAGATTTTG CATCTCATTG CTCCAAAGAG AAGAAAGAAG AAAAAGTGCC CTGTATTCTT
    ATGAACAACA TCCAGCAACT TCGAGTGCAA CTAGAAAAAA TGTTTGAAGC AATGGGAGGA
    AAGGAGCTGG ATGCAGAAAC AAGTGACATT CTTAAAGAGC TGCAGGTGAA ACTCAACAAT
    GTCCTAGATG AGCTAAGCAG AGTGTTTACC ACCAGTTTTC AGGCACAAAT TGAGGAGTGT
    GTGAAGCAGA TGGGTGACAT CCTGAGCCAG GTAAAAGGGA CCGGAAATGT ACCAGCCAGT
    AACTGCAGCA GTGTGGCTCA GGATGCAGAT AATGTATTGC AGCCTATCCT GGATCCTCTG
    GACAGCAACC TGACACTATT TGCCAAGATC TGTGAAAAGA CAGTCCTGAA GCGGGTACTA
    AAAGAACTCT GGAAGCTTGT GATGAATACA ATGGAGAAAA CGATTGTCCT TCCACCACTA
    ACAGACCAAA CAGGAACCCA CCTTATCATT AATGCAGCCA AGGAACTGGG CCAGTTATCC
    AAACTAAAGG ATCACATGGT GCGAGAGGAA GCCAAGAGCC TCACCCCTAA GCAGTGTGCA
    GTGGTGGAGC TTGCACTTGA TACAATTAAG CAATATTTCC ATGCTGGAGG TGTTGGTCTC
    AAGAAGACAT TCCTGGAGAA GAGTCCAGAC CTACAATCTC TACGCTATGC ACTTTCTCTT
    TACACACAAG CAACCGATAC GCTAATCAAA ACCTTCGTCC AGACACAGTC TGCTCAGGTT
    CATGGTGGTA AAGGTATTAG GTTTACACTT AATGAAGAGA CTTATCCAGA AAAGGGATCT
    GGGGTGGATG ATTCTGTTGG GGAAGTTTCT ATCCATGTGG AGTTATTTAC TCACCCAGGA
    ACAGGCGAGC ACAAGATCAG CGTAAAGGTT GTAGCGGCCA ATGACCTTAA ATGGCAGACG
    TCAGGGATTT TCCGTCCCTT TATTGAGGTA AACATGATTG GTCCGAATCT GAGTGACAAA
    AAAAGGAAAT TTACCACCAA GTCCAAGAAC AACAGCTGGG CCCCAAAATA TAACGAGAGC
    TTCCAATTTA CCCTGGGTAA TGAGACTGGT CCAGAGAGTT ATGAGCTGCA AGTATGTGTG
    AAAGATTACT GTTTTGCTCG GGAAGACCGC ACAGTGGGAA TGGCTGTCAT GCAACTGCGA
    GATCTGGCAC AAAGGGGTAG CTGTGCCTGT TGGCTCCCTC TTGGCCGCCG CATTCATATG
    GATGAGACAG GACTGACCAT CTTGCGCATA CTATCACAAA GAAACAATGA TGAGGTGGCC
    AAAGAGTTTG TCAAACTCAA ATCTGACACC CGCTCTCCAG AAGAGGGGAG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa48435
    Clone ID Related Accession (Same CDS sequence) XM_012954752.3
    Accession Version XM_012954752.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5100bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein unc-13 homolog A isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012954752.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCTTTGC TTTGCGTGGG AGTAAAAAAG GCCACGTTTG ATGGAGCACA AGAGAAGTTT 
    AATGCCTACG TAACCTTGAA GGTGCAAAAT GTGAAGAGCA CAACGATAGC GGTGCGGGGA
    AACCAGCCCT GCTGGGAACA GGATTTCATG TTTGAGATTA ATCGTTTAGA CCTTGGTCTA
    ACAGTGGAGG TCTGGAACAA AGGATTGATC TGGGACACCA TGGTGGGAAC AGTATGGATT
    CCTTTAAGGA CAATCCGACA ATCCAATGAG GAGGGTCCTG GGGAATGGCT GACACTTGAT
    TCACAGGTCA TAATGACAGA TAATGAGATT TGTGGTACCA AAGATCCAAC CTTCCACCGC
    ATCTTACTGG ATACCCGATT TGAACTTCCT CTAGATATAC CAGAAGAAGA AGCTCGATAC
    TGGGCAAAGA AACTTGAGCA ACTTAATGCA ATGAGGGACC AGGATGATTA TGTTTTTCAA
    GAAGAAATTC CGGAGAATCC TCTTCCCGTC CATGGATCTC AATGTTGTAA CTGGAATTAT
    TTCGGCTGGG GGGAGCAGCA CAATGAGGAT CCCGATAGTG CAGTGGATGA TCGGGATAGT
    GATTACCGCA GTGAAACCAG CAACAGTATC CCGCCCCCCT ACTACTCCAC CTCACAGCCT
    AATGCCTCTG TGCACCAGTA CCCTGTACGA CAGCAACACA GTTCTCATGG ATCTTACACT
    GATTCAGTGC ACAGCTATGA GATGGACTTC TCTGACCATC GTCCAATCAG CCCCACTGGA
    AGTAGTCGAT ATGCTTCATC AGGGGAGATT AGTCGTGGCT CCTCACAGCT CAGTGATGAC
    TTTGACCCAG ATGATGACAG CTTGCAAGGT TCAGAAATAG AGGAAGAAAG GGATAGAGAC
    TCTTATCACT CCTGTCATAG TTCTGTCAGC TGCCGCAAGG ACTCGCCAAA TTGGGAAGGA
    GAGGAGGAGC CTGAAGTTTA TAGTGAAGAT GAGGAAGAAT ATCCTGACAG TAGGGGGCAG
    CTCTCTGACC AGGAGCTGTA TGATGATGAC CAGCTTGAGG AAGAAGAGAA AAAAGGAAGA
    TTAGCATATG GGCAGGAGGA TTCCATCTAT CAGTCATTGG AGGCTGAGGA GAAGGAACCC
    CTGGATATTC CATTGGAGAA GGACAGGACA CCAGAAGCTG TGCCAGAGGA GGTAGTAGAG
    CCAACAGTAC CAGCAGTCCA GTCTCCCCAA GCTGCAGACA GTTACAGAGA AGAAGAACCT
    GGAATGTCCA GGGCCAAATC TAATTGGTTA AGAGCATTCA ACAAGGTGCG CCTGCAGCTG
    CAAGAGGCAC GAGGTGAAGG GACTGGGGAG ATGTCCAAAT CCCTCTGGTT TAAGGATGGG
    CCAGCTGGTG GTCTTATAAT TATAGACAGT ATGCCAGACA TACGAAAGAG GAAACCAATC
    CCGTTGGTCA GTGACCTGGC AATGTCACTG GTTCAGTCAC GGAAGGCTGG GATTACTTCT
    GCTCTTGCAA CAAGCACCTT AAACAATGAA GAGCTAAAAA ACCATGTTTA CAAAAAGACC
    CTGCAAGCTT TAATATACCC AATATCCAGC ACCACTCCCC ACAACTTTGA AGTTTGGACT
    GCCACAACTC CTACCTATTG TTATGAATGC GAAGGGCTTC TGTGGGGAAT CGCTCGGCAG
    GGCATGCGTT GCACAGAGTG TGGTGTCAAG TGCCATGAGA AGTGCCAGGA TCTGCTGAAT
    GCAGATTGTC TTCAAAGAGC TGCAGAGAAG AGCTCAAAAC ATGGTGCAGA AGACCGGACT
    CAGAATATTA TTATGGTCTT GAAGGATCGT ATGAAGATCC GGGAGAGGAA CAAGCCAGAA
    ATATTTGAGC TGATCCAGGA AGTATTTGGG GTATTAAAGG CTACACACGC TCAGCAGATA
    AAGGCTGTGA AACAGAGTGT GCTGGATGGT ACTTCCAAAT GGTCAGCAAA GATCAGCATA
    ACTGTTGTAT GTGCACAGGG TCTACAGGCC AAAGATAAGA CAGGCTCCAG TGACCCATAT
    GTTACTGTTC AAGTTGGGAA AACCAAGAAA AGAACCAAAA CAATCTATGG AAATCTTAAT
    CCTGTCTGGG AAGAGAATTT TCACTTTGAA TGCCACAACT CCTCAGATCG TATTAAAGTA
    CGCGTGTGGG ATGAAGATGA TGATATCAAA TCTCGTGTGA AGCAGAGATT TAAAAGAGAA
    TCAGATGACT TCCTTGGACA AACAATTATT GAAGTCAGAA CACTTAGCGG AGAGATGGAT
    GTGTGGTATA ACTTGGATAA GAGAACAGAT AAATCCGCTG TCTCTGGGGC AATCCGTCTT
    CATATAAGTG TGGAAATAAA AGGAGAGGAA AAGGTGGCTC CCTATCACGT GCAATACACA
    TGTCTACATG AGAACTTATT CCATTACCTG ACGGATGTGC AGAATAATGG TGTGGTCAAG
    ATTCCAGATG CAAAAGGAGA CGACGCCTGG AAAGTTTATT TTGATGAGAC AGCCCAGGAA
    ATTGTGGATG AATTTGCAAT GAGATATGGA GTGGAGTCCA TATATCAGGC CATGACGCAT
    TTTGCCTGCC TTTCATCTAA ATATATGTGT CCAGGAGTAC CAGCTGTAAT GAGTACTCTT
    CTTGCCAACA TTAATGCTTA CTATGCGCAC ACAACTGCAT CCACTAATGT GTCTGCCTCC
    GACCGATTCT CTGCCTCAAA CTTTGGGAAG GAGAGATTTG TAAAGCTGCT TGACCAGTTG
    CACAATTCCT TACGCATTGA CCTGTCCATG TATCGGAACA ACTTCCCTGC TAGCAGCCCT
    GAAAGACTCC AAGATCTCAA GTCCACTGTA GATTTACTAA CTAGCATAAC ATTTTTCCGG
    ATGAAGGTAC AAGAGCTTCA GAGCCCACCA CGGGCAAGCC AAGTTGTTAA GGACTGTGTG
    AAGGCATGCC TTAACTCAAC CTACGAATAT ATCTTTAACA ACTGCCATGA ACTTTATAGC
    CGAGAATATC AAAGTGACCC TGCTAAAAAA GGGGAGGTAC CCCCAGAAGA ACAGGGGCCA
    AGTATAAAGA ACTTAGATTT TTGGTCCAAA CTAATCACAT TGATTGTGTC TATCATAGAG
    GAAGATAAGA ACTCATATAC ACCTTGTCTA AACCAGTTTC CTCAGGAGCT AAATGTTGGT
    AAGATTAGTG CTGAGGTCAT GTGGAGTCTC TTTGCTCAAG ACATGAGATA TGCTATGGAA
    GAACACGACA AACACCGCCT GTGTAAGAGT GCAGACTACA TGAACTTGCA TTTTAAGGTC
    AAGTGGTTGT ACAATGAATA TGTAACTGAG CTACCATCTT TTAAGAGCAA GGTTCCAGAA
    TATCCCGCCT GGTTTGAGCC TTTTGTAATT CAGTGGCTGG ATGAAAATGA AGAGGTCTCC
    CGGGATTTTC TGCATGGAGC ACTTGAAAGA GATAAGAAGG ATGGGTTCCA GCAGACCTCT
    GAACATGCCC TGTTCTCCTG CTCTGTGGTG GATGTTTTCT CACAACTTAA CCAGAGCTTT
    GAGATCATTA AGAAGTTGGA GTGTCCAGAC CCACAAATTG TGGGGCATTA CATGCGTAGA
    TTTGCCAAGA CGATCAGTAA TGTTCTGCTG CAGTATGCTG AAATTATTTC CAAAGATTTT
    GCATCTCATT GCTCCAAAGA GAAGAAAGAA GAAAAAGTGC CCTGTATTCT TATGAACAAC
    ATCCAGCAAC TTCGAGTGCA ACTAGAAAAA ATGTTTGAAG CAATGGGAGG AAAGGAGCTG
    GATGCAGAAA CAAGTGACAT TCTTAAAGAG CTGCAGGTGA AACTCAACAA TGTCCTAGAT
    GAGCTAAGCA GAGTGTTTAC CACCAGTTTT CAGGCACAAA TTGAGGAGTG TGTGAAGCAG
    ATGGGTGACA TCCTGAGCCA GGTAAAAGGG ACCGGAAATG TACCAGCCAG TAACTGCAGC
    AGTGTGGCTC AGGATGCAGA TAATGTATTG CAGCCTATCC TGGATCCTCT GGACAGCAAC
    CTGACACTAT TTGCCAAGAT CTGTGAAAAG ACAGTCCTGA AGCGGGTACT AAAAGAACTC
    TGGAAGCTTG TGATGAATAC AATGGAGAAA ACGATTGTCC TTCCACCACT AACAGACCAA
    ACAATGATAG GGACGCTGCT GAGAAAACAT GGCAAGGGAA TAGAGAAGAG CAGGGTGAAG
    CTGCCGTACC ACGCAGATGG AACCCACCTT ATCATTAATG CAGCCAAGGA ACTGGGCCAG
    TTATCCAAAC TAAAGGATCA CATGGTGCGA GAGGAAGCCA AGAGCCTCAC CCCTAAGCAG
    TGTGCAGTGG TGGAGCTTGC ACTTGATACA ATTAAGCAAT ATTTCCATGC TGGAGGTGTT
    GGTCTCAAGA AGACATTCCT GGAGAAGAGT CCAGACCTAC AATCTCTACG CTATGCACTT
    TCTCTTTACA CACAAGCAAC CGATACGCTA ATCAAAACCT TCGTCCAGAC ACAGTCTGCT
    CAGGTTCATG GTGGTAAAGG TATTAGGTTT ACACTTAATG AAGAGACTTA TCCAGAAAAG
    GGATCTGGGG TGGATGATTC TGTTGGGGAA GTTTCTATCC ATGTGGAGTT ATTTACTCAC
    CCAGGAACAG GCGAGCACAA GATCAGCGTA AAGGTTGTAG CGGCCAATGA CCTTAAATGG
    CAGACGTCAG GGATTTTCCG TCCCTTTATT GAGGTAAACA TGATTGGTCC GAATCTGAGT
    GACAAAAAAA GGAAATTTAC CACCAAGTCC AAGAACAACA GCTGGGCCCC AAAATATAAC
    GAGAGCTTCC AATTTACCCT GGGTAATGAG ACTGGTCCAG AGAGTTATGA GCTGCAAGTA
    TGTGTGAAAG ATTACTGTTT TGCTCGGGAA GACCGCACAG TGGGAATGGC TGTCATGCAA
    CTGCGAGATC TGGCACAAAG GGGTAGCTGT GCCTGTTGGC TCCCTCTTGG CCGCCGCATT
    CATATGGATG AGACAGGACT GACCATCTTG CGCATACTAT CACAAAGAAA CAATGATGAG
    GTGGCCAAAG AGTTTGTCAA ACTCAAATCT GACACCCGCT CTCCAGAAGA GGGGAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012810206.2
    CDS187..5286
    Translation

    Target ORF information:

    RefSeq Version XM_012954752.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis unc-13 homolog A (unc13a), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012954752.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCTTTGC TTTGCGTGGG AGTAAAAAAG GCCACGTTTG ATGGAGCACA AGAGAAGTTT 
    AATGCCTACG TAACCTTGAA GGTGCAAAAT GTGAAGAGCA CAACGATAGC GGTGCGGGGA
    AACCAGCCCT GCTGGGAACA GGATTTCATG TTTGAGATTA ATCGTTTAGA CCTTGGTCTA
    ACAGTGGAGG TCTGGAACAA AGGATTGATC TGGGACACCA TGGTGGGAAC AGTATGGATT
    CCTTTAAGGA CAATCCGACA ATCCAATGAG GAGGGTCCTG GGGAATGGCT GACACTTGAT
    TCACAGGTCA TAATGACAGA TAATGAGATT TGTGGTACCA AAGATCCAAC CTTCCACCGC
    ATCTTACTGG ATACCCGATT TGAACTTCCT CTAGATATAC CAGAAGAAGA AGCTCGATAC
    TGGGCAAAGA AACTTGAGCA ACTTAATGCA ATGAGGGACC AGGATGATTA TGTTTTTCAA
    GAAGAAATTC CGGAGAATCC TCTTCCCGTC CATGGATCTC AATGTTGTAA CTGGAATTAT
    TTCGGCTGGG GGGAGCAGCA CAATGAGGAT CCCGATAGTG CAGTGGATGA TCGGGATAGT
    GATTACCGCA GTGAAACCAG CAACAGTATC CCGCCCCCCT ACTACTCCAC CTCACAGCCT
    AATGCCTCTG TGCACCAGTA CCCTGTACGA CAGCAACACA GTTCTCATGG ATCTTACACT
    GATTCAGTGC ACAGCTATGA GATGGACTTC TCTGACCATC GTCCAATCAG CCCCACTGGA
    AGTAGTCGAT ATGCTTCATC AGGGGAGATT AGTCGTGGCT CCTCACAGCT CAGTGATGAC
    TTTGACCCAG ATGATGACAG CTTGCAAGGT TCAGAAATAG AGGAAGAAAG GGATAGAGAC
    TCTTATCACT CCTGTCATAG TTCTGTCAGC TGCCGCAAGG ACTCGCCAAA TTGGGAAGGA
    GAGGAGGAGC CTGAAGTTTA TAGTGAAGAT GAGGAAGAAT ATCCTGACAG TAGGGGGCAG
    CTCTCTGACC AGGAGCTGTA TGATGATGAC CAGCTTGAGG AAGAAGAGAA AAAAGGAAGA
    TTAGCATATG GGCAGGAGGA TTCCATCTAT CAGTCATTGG AGGCTGAGGA GAAGGAACCC
    CTGGATATTC CATTGGAGAA GGACAGGACA CCAGAAGCTG TGCCAGAGGA GGTAGTAGAG
    CCAACAGTAC CAGCAGTCCA GTCTCCCCAA GCTGCAGACA GTTACAGAGA AGAAGAACCT
    GGAATGTCCA GGGCCAAATC TAATTGGTTA AGAGCATTCA ACAAGGTGCG CCTGCAGCTG
    CAAGAGGCAC GAGGTGAAGG GACTGGGGAG ATGTCCAAAT CCCTCTGGTT TAAGGATGGG
    CCAGCTGGTG GTCTTATAAT TATAGACAGT ATGCCAGACA TACGAAAGAG GAAACCAATC
    CCGTTGGTCA GTGACCTGGC AATGTCACTG GTTCAGTCAC GGAAGGCTGG GATTACTTCT
    GCTCTTGCAA CAAGCACCTT AAACAATGAA GAGCTAAAAA ACCATGTTTA CAAAAAGACC
    CTGCAAGCTT TAATATACCC AATATCCAGC ACCACTCCCC ACAACTTTGA AGTTTGGACT
    GCCACAACTC CTACCTATTG TTATGAATGC GAAGGGCTTC TGTGGGGAAT CGCTCGGCAG
    GGCATGCGTT GCACAGAGTG TGGTGTCAAG TGCCATGAGA AGTGCCAGGA TCTGCTGAAT
    GCAGATTGTC TTCAAAGAGC TGCAGAGAAG AGCTCAAAAC ATGGTGCAGA AGACCGGACT
    CAGAATATTA TTATGGTCTT GAAGGATCGT ATGAAGATCC GGGAGAGGAA CAAGCCAGAA
    ATATTTGAGC TGATCCAGGA AGTATTTGGG GTATTAAAGG CTACACACGC TCAGCAGATA
    AAGGCTGTGA AACAGAGTGT GCTGGATGGT ACTTCCAAAT GGTCAGCAAA GATCAGCATA
    ACTGTTGTAT GTGCACAGGG TCTACAGGCC AAAGATAAGA CAGGCTCCAG TGACCCATAT
    GTTACTGTTC AAGTTGGGAA AACCAAGAAA AGAACCAAAA CAATCTATGG AAATCTTAAT
    CCTGTCTGGG AAGAGAATTT TCACTTTGAA TGCCACAACT CCTCAGATCG TATTAAAGTA
    CGCGTGTGGG ATGAAGATGA TGATATCAAA TCTCGTGTGA AGCAGAGATT TAAAAGAGAA
    TCAGATGACT TCCTTGGACA AACAATTATT GAAGTCAGAA CACTTAGCGG AGAGATGGAT
    GTGTGGTATA ACTTGGATAA GAGAACAGAT AAATCCGCTG TCTCTGGGGC AATCCGTCTT
    CATATAAGTG TGGAAATAAA AGGAGAGGAA AAGGTGGCTC CCTATCACGT GCAATACACA
    TGTCTACATG AGAACTTATT CCATTACCTG ACGGATGTGC AGAATAATGG TGTGGTCAAG
    ATTCCAGATG CAAAAGGAGA CGACGCCTGG AAAGTTTATT TTGATGAGAC AGCCCAGGAA
    ATTGTGGATG AATTTGCAAT GAGATATGGA GTGGAGTCCA TATATCAGGC CATGACGCAT
    TTTGCCTGCC TTTCATCTAA ATATATGTGT CCAGGAGTAC CAGCTGTAAT GAGTACTCTT
    CTTGCCAACA TTAATGCTTA CTATGCGCAC ACAACTGCAT CCACTAATGT GTCTGCCTCC
    GACCGATTCT CTGCCTCAAA CTTTGGGAAG GAGAGATTTG TAAAGCTGCT TGACCAGTTG
    CACAATTCCT TACGCATTGA CCTGTCCATG TATCGGAACA ACTTCCCTGC TAGCAGCCCT
    GAAAGACTCC AAGATCTCAA GTCCACTGTA GATTTACTAA CTAGCATAAC ATTTTTCCGG
    ATGAAGGTAC AAGAGCTTCA GAGCCCACCA CGGGCAAGCC AAGTTGTTAA GGACTGTGTG
    AAGGCATGCC TTAACTCAAC CTACGAATAT ATCTTTAACA ACTGCCATGA ACTTTATAGC
    CGAGAATATC AAAGTGACCC TGCTAAAAAA GGGGAGGTAC CCCCAGAAGA ACAGGGGCCA
    AGTATAAAGA ACTTAGATTT TTGGTCCAAA CTAATCACAT TGATTGTGTC TATCATAGAG
    GAAGATAAGA ACTCATATAC ACCTTGTCTA AACCAGTTTC CTCAGGAGCT AAATGTTGGT
    AAGATTAGTG CTGAGGTCAT GTGGAGTCTC TTTGCTCAAG ACATGAGATA TGCTATGGAA
    GAACACGACA AACACCGCCT GTGTAAGAGT GCAGACTACA TGAACTTGCA TTTTAAGGTC
    AAGTGGTTGT ACAATGAATA TGTAACTGAG CTACCATCTT TTAAGAGCAA GGTTCCAGAA
    TATCCCGCCT GGTTTGAGCC TTTTGTAATT CAGTGGCTGG ATGAAAATGA AGAGGTCTCC
    CGGGATTTTC TGCATGGAGC ACTTGAAAGA GATAAGAAGG ATGGGTTCCA GCAGACCTCT
    GAACATGCCC TGTTCTCCTG CTCTGTGGTG GATGTTTTCT CACAACTTAA CCAGAGCTTT
    GAGATCATTA AGAAGTTGGA GTGTCCAGAC CCACAAATTG TGGGGCATTA CATGCGTAGA
    TTTGCCAAGA CGATCAGTAA TGTTCTGCTG CAGTATGCTG AAATTATTTC CAAAGATTTT
    GCATCTCATT GCTCCAAAGA GAAGAAAGAA GAAAAAGTGC CCTGTATTCT TATGAACAAC
    ATCCAGCAAC TTCGAGTGCA ACTAGAAAAA ATGTTTGAAG CAATGGGAGG AAAGGAGCTG
    GATGCAGAAA CAAGTGACAT TCTTAAAGAG CTGCAGGTGA AACTCAACAA TGTCCTAGAT
    GAGCTAAGCA GAGTGTTTAC CACCAGTTTT CAGGCACAAA TTGAGGAGTG TGTGAAGCAG
    ATGGGTGACA TCCTGAGCCA GGTAAAAGGG ACCGGAAATG TACCAGCCAG TAACTGCAGC
    AGTGTGGCTC AGGATGCAGA TAATGTATTG CAGCCTATCC TGGATCCTCT GGACAGCAAC
    CTGACACTAT TTGCCAAGAT CTGTGAAAAG ACAGTCCTGA AGCGGGTACT AAAAGAACTC
    TGGAAGCTTG TGATGAATAC AATGGAGAAA ACGATTGTCC TTCCACCACT AACAGACCAA
    ACAATGATAG GGACGCTGCT GAGAAAACAT GGCAAGGGAA TAGAGAAGAG CAGGGTGAAG
    CTGCCGTACC ACGCAGATGG AACCCACCTT ATCATTAATG CAGCCAAGGA ACTGGGCCAG
    TTATCCAAAC TAAAGGATCA CATGGTGCGA GAGGAAGCCA AGAGCCTCAC CCCTAAGCAG
    TGTGCAGTGG TGGAGCTTGC ACTTGATACA ATTAAGCAAT ATTTCCATGC TGGAGGTGTT
    GGTCTCAAGA AGACATTCCT GGAGAAGAGT CCAGACCTAC AATCTCTACG CTATGCACTT
    TCTCTTTACA CACAAGCAAC CGATACGCTA ATCAAAACCT TCGTCCAGAC ACAGTCTGCT
    CAGGTTCATG GTGGTAAAGG TATTAGGTTT ACACTTAATG AAGAGACTTA TCCAGAAAAG
    GGATCTGGGG TGGATGATTC TGTTGGGGAA GTTTCTATCC ATGTGGAGTT ATTTACTCAC
    CCAGGAACAG GCGAGCACAA GATCAGCGTA AAGGTTGTAG CGGCCAATGA CCTTAAATGG
    CAGACGTCAG GGATTTTCCG TCCCTTTATT GAGGTAAACA TGATTGGTCC GAATCTGAGT
    GACAAAAAAA GGAAATTTAC CACCAAGTCC AAGAACAACA GCTGGGCCCC AAAATATAAC
    GAGAGCTTCC AATTTACCCT GGGTAATGAG ACTGGTCCAG AGAGTTATGA GCTGCAAGTA
    TGTGTGAAAG ATTACTGTTT TGCTCGGGAA GACCGCACAG TGGGAATGGC TGTCATGCAA
    CTGCGAGATC TGGCACAAAG GGGTAGCTGT GCCTGTTGGC TCCCTCTTGG CCGCCGCATT
    CATATGGATG AGACAGGACT GACCATCTTG CGCATACTAT CACAAAGAAA CAATGATGAG
    GTGGCCAAAG AGTTTGTCAA ACTCAAATCT GACACCCGCT CTCCAGAAGA GGGGAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa48436
    Clone ID Related Accession (Same CDS sequence) XM_031904669.1
    Accession Version XM_031904669.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5097bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein unc-13 homolog A isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCTTTGC TTTGCGTGGG AGTAAAAAAG GCCACGTTTG ATGGAGCACA AGAGAAGTTT 
    AATGCCTACG TAACCTTGAA GGTGCAAAAT GTGAAGAGCA CAACGATAGC GGTGCGGGGA
    AACCAGCCCT GCTGGGAACA GGATTTCATG TTTGAGATTA ATCGTTTAGA CCTTGGTCTA
    ACAGTGGAGG TCTGGAACAA AGGATTGATC TGGGACACCA TGGTGGGAAC AGTATGGATT
    CCTTTAAGGA CAATCCGACA ATCCAATGAG GAGGGTCCTG GGGAATGGCT GACACTTGAT
    TCACAGGTCA TAATGACAGA TAATGAGATT TGTGGTACCA AAGATCCAAC CTTCCACCGC
    ATCTTACTGG ATACCCGATT TGAACTTCCT CTAGATATAC CAGAAGAAGA AGCTCGATAC
    TGGGCAAAGA AACTTGAGCA ACTTAATGCA ATGAGGGACC AGGATGATTA TGTTTTTCAA
    GAAGAAATTC CGGAGAATCC TCTTCCCGTC CATGGATCTC AATGTTGTAA CTGGAATTAT
    TTCGGCTGGG GGGAGCAGCA CAATGAGGAT CCCGATAGTG CAGTGGATGA TCGGGATAGT
    GATTACCGCA GTGAAACCAG CAACAGTATC CCGCCCCCCT ACTACTCCAC CTCACAGCCT
    AATGCCTCTG TGCACCAGTA CCCTGTACGA CAGCAACACA GTTCTCATGG ATCTTACACT
    GATTCAGTGC ACAGCTATGA GATGGACTTC TCTGACCATC GTCCAATCAG CAGGCGTTAC
    GATAGCGCAG ACTCTGCCAC TAACGGGCGC AGTGATGTGG AGTCTGTCTC CGGTTCGAGC
    CGCCTCACCA GCCGGCAGCA CTCTCTGGAG AGCCGGCAGT CCTTAGACCT GTCCGATAGC
    CCCACTGGAA GTAGTCGATA TGCTTCATCA GGGGAGATTA GTCGTGGCTC CTCACAGCTC
    AGTGATGACT TTGACCCAGA TGATGACAGC TTGCAAGGTT CAGAAATAGA GGAAGAAAGG
    GATAGAGACT CTTATCACTC CTGTCATAGT TCTGTCAGCT GCCGCAAGGA CTCGCCAAAT
    TGGGAAGGAG AGGAGGAGCC TGAAGTTTAT AGTGAAGATG AGGAAGAATA TCCTGACAGT
    AGGGGGCAGC TCTCTGACCA GGAGCTGTAT GATGATGACC AGCTTGAGGA AGAAGAGAAA
    AAAGGAAGAT TAGCATATGG GCAGGAGGAT TCCATCTATC AGTCATTGGA GGCTGAGGAG
    AAGGAACCCC TGGATATTCC ATTGGAGAAG GACAGGACAC CAGAAGCTGT GCCAGAGGAG
    GTAGTAGAGC CAACAGTACC AGCAGTCCAG TCTCCCCAAG CTGCAGACAG TTACAGAGAA
    GAAGAACCTG GAATGTCCAG GGCCAAATCT AATTGGTTAA GAGCATTCAA CAAGGTGCGC
    CTGCAGCTGC AAGAGGCACG AGGTGAAGGG ACTGGGGAGA TGTCCAAATC CCTCTGGTTT
    AAGGATGGGC CAGCTGGTGG TCTTATAATT ATAGACAGTA TGCCAGACAT ACGAAAGAGG
    AAACCAATCC CGTTGGTCAG TGACCTGGCA ATGTCACTGG TTCAGTCACG GAAGGCTGGG
    ATTACTTCTG CTCTTGCAAC AAGCACCTTA AACAATGAAG AGCTAAAAAA CCATGTTTAC
    AAAAAGACCC TGCAAGCTTT AATATACCCA ATATCCAGCA CCACTCCCCA CAACTTTGAA
    GTTTGGACTG CCACAACTCC TACCTATTGT TATGAATGCG AAGGGCTTCT GTGGGGAATC
    GCTCGGCAGG GCATGCGTTG CACAGAGTGT GGTGTCAAGT GCCATGAGAA GTGCCAGGAT
    CTGCTGAATG CAGATTGTCT TCAAAGAGCT GCAGAGAAGA GCTCAAAACA TGGTGCAGAA
    GACCGGACTC AGAATATTAT TATGGTCTTG AAGGATCGTA TGAAGATCCG GGAGAGGAAC
    AAGCCAGAAA TATTTGAGCT GATCCAGGAA GTATTTGGGG TATTAAAGGC TACACACGCT
    CAGCAGATAA AGGCTGTGAA ACAGAGTGTG CTGGATGGTA CTTCCAAATG GTCAGCAAAG
    ATCAGCATAA CTGTTGTATG TGCACAGGGT CTACAGGCCA AAGATAAGAC AGGCTCCAGT
    GACCCATATG TTACTGTTCA AGTTGGGAAA ACCAAGAAAA GAACCAAAAC AATCTATGGA
    AATCTTAATC CTGTCTGGGA AGAGAATTTT CACTTTGAAT GCCACAACTC CTCAGATCGT
    ATTAAAGTAC GCGTGTGGGA TGAAGATGAT GATATCAAAT CTCGTGTGAA GCAGAGATTT
    AAAAGAGAAT CAGATGACTT CCTTGGACAA ACAATTATTG AAGTCAGAAC ACTTAGCGGA
    GAGATGGATG TGTGGTATAA CTTGGATAAG AGAACAGATA AATCCGCTGT CTCTGGGGCA
    ATCCGTCTTC ATATAAGTGT GGAAATAAAA GGAGAGGAAA AGGTGGCTCC CTATCACGTG
    CAATACACAT GTCTACATGA GAACTTATTC CATTACCTGA CGGATGTGCA GAATAATGGT
    GTGGTCAAGA TTCCAGATGC AAAAGGAGAC GACGCCTGGA AAGTTTATTT TGATGAGACA
    GCCCAGGAAA TTGTGGATGA ATTTGCAATG AGATATGGAG TGGAGTCCAT ATATCAGGCC
    ATGACGCATT TTGCCTGCCT TTCATCTAAA TATATGTGTC CAGGAGTACC AGCTGTAATG
    AGTACTCTTC TTGCCAACAT TAATGCTTAC TATGCGCACA CAACTGCATC CACTAATGTG
    TCTGCCTCCG ACCGATTCTC TGCCTCAAAC TTTGGGAAGG AGAGATTTGT AAAGCTGCTT
    GACCAGTTGC ACAATTCCTT ACGCATTGAC CTGTCCATGT ATCGGAACAA CTTCCCTGCT
    AGCAGCCCTG AAAGACTCCA AGATCTCAAG TCCACTGTAG ATTTACTAAC TAGCATAACA
    TTTTTCCGGA TGAAGGTACA AGAGCTTCAG AGCCCACCAC GGGCAAGCCA AGTTGTTAAG
    GACTGTGTGA AGGCATGCCT TAACTCAACC TACGAATATA TCTTTAACAA CTGCCATGAA
    CTTTATAGCC GAGAATATCA AAGTGACCCT GCTAAAAAAG GGGAGGTACC CCCAGAAGAA
    CAGGGGCCAA GTATAAAGAA CTTAGATTTT TGGTCCAAAC TAATCACATT GATTGTGTCT
    ATCATAGAGG AAGATAAGAA CTCATATACA CCTTGTCTAA ACCAGTTTCC TCAGGAGCTA
    AATGTTGGTA AGATTAGTGC TGAGGTCATG TGGAGTCTCT TTGCTCAAGA CATGAGATAT
    GCTATGGAAG AACACGACAA ACACCGCCTG TGTAAGAGTG CAGACTACAT GAACTTGCAT
    TTTAAGGTCA AGTGGTTGTA CAATGAATAT GTAACTGAGC TACCATCTTT TAAGAGCAAG
    GTTCCAGAAT ATCCCGCCTG GTTTGAGCCT TTTGTAATTC AGTGGCTGGA TGAAAATGAA
    GAGGTCTCCC GGGATTTTCT GCATGGAGCA CTTGAAAGAG ATAAGAAGGA TGGGTTCCAG
    CAGACCTCTG AACATGCCCT GTTCTCCTGC TCTGTGGTGG ATGTTTTCTC ACAACTTAAC
    CAGAGCTTTG AGATCATTAA GAAGTTGGAG TGTCCAGACC CACAAATTGT GGGGCATTAC
    ATGCGTAGAT TTGCCAAGAC GATCAGTAAT GTTCTGCTGC AGTATGCTGA AATTATTTCC
    AAAGATTTTG CATCTCATTG CTCCAAAGAG AAGAAAGAAG AAAAAGTGCC CTGTATTCTT
    ATGAACAACA TCCAGCAACT TCGAGTGCAA CTAGAAAAAA TGTTTGAAGC AATGGGAGGA
    AAGGAGCTGG ATGCAGAAAC AAGTGACATT CTTAAAGAGC TGCAGGTGAA ACTCAACAAT
    GTCCTAGATG AGCTAAGCAG AGTGTTTACC ACCAGTTTTC AGGCACAAAT TGAGGAGTGT
    GTGAAGCAGA TGGGTGACAT CCTGAGCCAG GTAAAAGGGA CCGGAAATGT ACCAGCCAGT
    AACTGCAGCA GTGTGGCTCA GGATGCAGAT AATGTATTGC AGCCTATCCT GGATCCTCTG
    GACAGCAACC TGACACTATT TGCCAAGATC TGTGAAAAGA CAGTCCTGAA GCGGGTACTA
    AAAGAACTCT GGAAGCTTGT GATGAATACA ATGGAGAAAA CGATTGTCCT TCCACCACTA
    ACAGACCAAA CAGGAACCCA CCTTATCATT AATGCAGCCA AGGAACTGGG CCAGTTATCC
    AAACTAAAGG ATCACATGGT GCGAGAGGAA GCCAAGAGCC TCACCCCTAA GCAGTGTGCA
    GTGGTGGAGC TTGCACTTGA TACAATTAAG CAATATTTCC ATGCTGGAGG TGTTGGTCTC
    AAGAAGACAT TCCTGGAGAA GAGTCCAGAC CTACAATCTC TACGCTATGC ACTTTCTCTT
    TACACACAAG CAACCGATAC GCTAATCAAA ACCTTCGTCC AGACACAGTC TGCTCAGGGA
    TCTGGGGTGG ATGATTCTGT TGGGGAAGTT TCTATCCATG TGGAGTTATT TACTCACCCA
    GGAACAGGCG AGCACAAGAT CAGCGTAAAG GTTGTAGCGG CCAATGACCT TAAATGGCAG
    ACGTCAGGGA TTTTCCGTCC CTTTATTGAG GTAAACATGA TTGGTCCGAA TCTGAGTGAC
    AAAAAAAGGA AATTTACCAC CAAGTCCAAG AACAACAGCT GGGCCCCAAA ATATAACGAG
    AGCTTCCAAT TTACCCTGGG TAATGAGACT GGTCCAGAGA GTTATGAGCT GCAAGTATGT
    GTGAAAGATT ACTGTTTTGC TCGGGAAGAC CGCACAGTGG GAATGGCTGT CATGCAACTG
    CGAGATCTGG CACAAAGGGG TAGCTGTGCC TGTTGGCTCC CTCTTGGCCG CCGCATTCAT
    ATGGATGAGA CAGGACTGAC CATCTTGCGC ATACTATCAC AAAGAAACAA TGATGAGGTG
    GCCAAAGAGT TTGTCAAACT CAAATCTGAC ACCCGCTCTC CAGAAGAGGG GAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031760529.1
    CDS187..5283
    Translation

    Target ORF information:

    RefSeq Version XM_031904669.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis unc-13 homolog A (unc13a), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031904669.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCTTTGC TTTGCGTGGG AGTAAAAAAG GCCACGTTTG ATGGAGCACA AGAGAAGTTT 
    AATGCCTACG TAACCTTGAA GGTGCAAAAT GTGAAGAGCA CAACGATAGC GGTGCGGGGA
    AACCAGCCCT GCTGGGAACA GGATTTCATG TTTGAGATTA ATCGTTTAGA CCTTGGTCTA
    ACAGTGGAGG TCTGGAACAA AGGATTGATC TGGGACACCA TGGTGGGAAC AGTATGGATT
    CCTTTAAGGA CAATCCGACA ATCCAATGAG GAGGGTCCTG GGGAATGGCT GACACTTGAT
    TCACAGGTCA TAATGACAGA TAATGAGATT TGTGGTACCA AAGATCCAAC CTTCCACCGC
    ATCTTACTGG ATACCCGATT TGAACTTCCT CTAGATATAC CAGAAGAAGA AGCTCGATAC
    TGGGCAAAGA AACTTGAGCA ACTTAATGCA ATGAGGGACC AGGATGATTA TGTTTTTCAA
    GAAGAAATTC CGGAGAATCC TCTTCCCGTC CATGGATCTC AATGTTGTAA CTGGAATTAT
    TTCGGCTGGG GGGAGCAGCA CAATGAGGAT CCCGATAGTG CAGTGGATGA TCGGGATAGT
    GATTACCGCA GTGAAACCAG CAACAGTATC CCGCCCCCCT ACTACTCCAC CTCACAGCCT
    AATGCCTCTG TGCACCAGTA CCCTGTACGA CAGCAACACA GTTCTCATGG ATCTTACACT
    GATTCAGTGC ACAGCTATGA GATGGACTTC TCTGACCATC GTCCAATCAG CAGGCGTTAC
    GATAGCGCAG ACTCTGCCAC TAACGGGCGC AGTGATGTGG AGTCTGTCTC CGGTTCGAGC
    CGCCTCACCA GCCGGCAGCA CTCTCTGGAG AGCCGGCAGT CCTTAGACCT GTCCGATAGC
    CCCACTGGAA GTAGTCGATA TGCTTCATCA GGGGAGATTA GTCGTGGCTC CTCACAGCTC
    AGTGATGACT TTGACCCAGA TGATGACAGC TTGCAAGGTT CAGAAATAGA GGAAGAAAGG
    GATAGAGACT CTTATCACTC CTGTCATAGT TCTGTCAGCT GCCGCAAGGA CTCGCCAAAT
    TGGGAAGGAG AGGAGGAGCC TGAAGTTTAT AGTGAAGATG AGGAAGAATA TCCTGACAGT
    AGGGGGCAGC TCTCTGACCA GGAGCTGTAT GATGATGACC AGCTTGAGGA AGAAGAGAAA
    AAAGGAAGAT TAGCATATGG GCAGGAGGAT TCCATCTATC AGTCATTGGA GGCTGAGGAG
    AAGGAACCCC TGGATATTCC ATTGGAGAAG GACAGGACAC CAGAAGCTGT GCCAGAGGAG
    GTAGTAGAGC CAACAGTACC AGCAGTCCAG TCTCCCCAAG CTGCAGACAG TTACAGAGAA
    GAAGAACCTG GAATGTCCAG GGCCAAATCT AATTGGTTAA GAGCATTCAA CAAGGTGCGC
    CTGCAGCTGC AAGAGGCACG AGGTGAAGGG ACTGGGGAGA TGTCCAAATC CCTCTGGTTT
    AAGGATGGGC CAGCTGGTGG TCTTATAATT ATAGACAGTA TGCCAGACAT ACGAAAGAGG
    AAACCAATCC CGTTGGTCAG TGACCTGGCA ATGTCACTGG TTCAGTCACG GAAGGCTGGG
    ATTACTTCTG CTCTTGCAAC AAGCACCTTA AACAATGAAG AGCTAAAAAA CCATGTTTAC
    AAAAAGACCC TGCAAGCTTT AATATACCCA ATATCCAGCA CCACTCCCCA CAACTTTGAA
    GTTTGGACTG CCACAACTCC TACCTATTGT TATGAATGCG AAGGGCTTCT GTGGGGAATC
    GCTCGGCAGG GCATGCGTTG CACAGAGTGT GGTGTCAAGT GCCATGAGAA GTGCCAGGAT
    CTGCTGAATG CAGATTGTCT TCAAAGAGCT GCAGAGAAGA GCTCAAAACA TGGTGCAGAA
    GACCGGACTC AGAATATTAT TATGGTCTTG AAGGATCGTA TGAAGATCCG GGAGAGGAAC
    AAGCCAGAAA TATTTGAGCT GATCCAGGAA GTATTTGGGG TATTAAAGGC TACACACGCT
    CAGCAGATAA AGGCTGTGAA ACAGAGTGTG CTGGATGGTA CTTCCAAATG GTCAGCAAAG
    ATCAGCATAA CTGTTGTATG TGCACAGGGT CTACAGGCCA AAGATAAGAC AGGCTCCAGT
    GACCCATATG TTACTGTTCA AGTTGGGAAA ACCAAGAAAA GAACCAAAAC AATCTATGGA
    AATCTTAATC CTGTCTGGGA AGAGAATTTT CACTTTGAAT GCCACAACTC CTCAGATCGT
    ATTAAAGTAC GCGTGTGGGA TGAAGATGAT GATATCAAAT CTCGTGTGAA GCAGAGATTT
    AAAAGAGAAT CAGATGACTT CCTTGGACAA ACAATTATTG AAGTCAGAAC ACTTAGCGGA
    GAGATGGATG TGTGGTATAA CTTGGATAAG AGAACAGATA AATCCGCTGT CTCTGGGGCA
    ATCCGTCTTC ATATAAGTGT GGAAATAAAA GGAGAGGAAA AGGTGGCTCC CTATCACGTG
    CAATACACAT GTCTACATGA GAACTTATTC CATTACCTGA CGGATGTGCA GAATAATGGT
    GTGGTCAAGA TTCCAGATGC AAAAGGAGAC GACGCCTGGA AAGTTTATTT TGATGAGACA
    GCCCAGGAAA TTGTGGATGA ATTTGCAATG AGATATGGAG TGGAGTCCAT ATATCAGGCC
    ATGACGCATT TTGCCTGCCT TTCATCTAAA TATATGTGTC CAGGAGTACC AGCTGTAATG
    AGTACTCTTC TTGCCAACAT TAATGCTTAC TATGCGCACA CAACTGCATC CACTAATGTG
    TCTGCCTCCG ACCGATTCTC TGCCTCAAAC TTTGGGAAGG AGAGATTTGT AAAGCTGCTT
    GACCAGTTGC ACAATTCCTT ACGCATTGAC CTGTCCATGT ATCGGAACAA CTTCCCTGCT
    AGCAGCCCTG AAAGACTCCA AGATCTCAAG TCCACTGTAG ATTTACTAAC TAGCATAACA
    TTTTTCCGGA TGAAGGTACA AGAGCTTCAG AGCCCACCAC GGGCAAGCCA AGTTGTTAAG
    GACTGTGTGA AGGCATGCCT TAACTCAACC TACGAATATA TCTTTAACAA CTGCCATGAA
    CTTTATAGCC GAGAATATCA AAGTGACCCT GCTAAAAAAG GGGAGGTACC CCCAGAAGAA
    CAGGGGCCAA GTATAAAGAA CTTAGATTTT TGGTCCAAAC TAATCACATT GATTGTGTCT
    ATCATAGAGG AAGATAAGAA CTCATATACA CCTTGTCTAA ACCAGTTTCC TCAGGAGCTA
    AATGTTGGTA AGATTAGTGC TGAGGTCATG TGGAGTCTCT TTGCTCAAGA CATGAGATAT
    GCTATGGAAG AACACGACAA ACACCGCCTG TGTAAGAGTG CAGACTACAT GAACTTGCAT
    TTTAAGGTCA AGTGGTTGTA CAATGAATAT GTAACTGAGC TACCATCTTT TAAGAGCAAG
    GTTCCAGAAT ATCCCGCCTG GTTTGAGCCT TTTGTAATTC AGTGGCTGGA TGAAAATGAA
    GAGGTCTCCC GGGATTTTCT GCATGGAGCA CTTGAAAGAG ATAAGAAGGA TGGGTTCCAG
    CAGACCTCTG AACATGCCCT GTTCTCCTGC TCTGTGGTGG ATGTTTTCTC ACAACTTAAC
    CAGAGCTTTG AGATCATTAA GAAGTTGGAG TGTCCAGACC CACAAATTGT GGGGCATTAC
    ATGCGTAGAT TTGCCAAGAC GATCAGTAAT GTTCTGCTGC AGTATGCTGA AATTATTTCC
    AAAGATTTTG CATCTCATTG CTCCAAAGAG AAGAAAGAAG AAAAAGTGCC CTGTATTCTT
    ATGAACAACA TCCAGCAACT TCGAGTGCAA CTAGAAAAAA TGTTTGAAGC AATGGGAGGA
    AAGGAGCTGG ATGCAGAAAC AAGTGACATT CTTAAAGAGC TGCAGGTGAA ACTCAACAAT
    GTCCTAGATG AGCTAAGCAG AGTGTTTACC ACCAGTTTTC AGGCACAAAT TGAGGAGTGT
    GTGAAGCAGA TGGGTGACAT CCTGAGCCAG GTAAAAGGGA CCGGAAATGT ACCAGCCAGT
    AACTGCAGCA GTGTGGCTCA GGATGCAGAT AATGTATTGC AGCCTATCCT GGATCCTCTG
    GACAGCAACC TGACACTATT TGCCAAGATC TGTGAAAAGA CAGTCCTGAA GCGGGTACTA
    AAAGAACTCT GGAAGCTTGT GATGAATACA ATGGAGAAAA CGATTGTCCT TCCACCACTA
    ACAGACCAAA CAGGAACCCA CCTTATCATT AATGCAGCCA AGGAACTGGG CCAGTTATCC
    AAACTAAAGG ATCACATGGT GCGAGAGGAA GCCAAGAGCC TCACCCCTAA GCAGTGTGCA
    GTGGTGGAGC TTGCACTTGA TACAATTAAG CAATATTTCC ATGCTGGAGG TGTTGGTCTC
    AAGAAGACAT TCCTGGAGAA GAGTCCAGAC CTACAATCTC TACGCTATGC ACTTTCTCTT
    TACACACAAG CAACCGATAC GCTAATCAAA ACCTTCGTCC AGACACAGTC TGCTCAGGGA
    TCTGGGGTGG ATGATTCTGT TGGGGAAGTT TCTATCCATG TGGAGTTATT TACTCACCCA
    GGAACAGGCG AGCACAAGAT CAGCGTAAAG GTTGTAGCGG CCAATGACCT TAAATGGCAG
    ACGTCAGGGA TTTTCCGTCC CTTTATTGAG GTAAACATGA TTGGTCCGAA TCTGAGTGAC
    AAAAAAAGGA AATTTACCAC CAAGTCCAAG AACAACAGCT GGGCCCCAAA ATATAACGAG
    AGCTTCCAAT TTACCCTGGG TAATGAGACT GGTCCAGAGA GTTATGAGCT GCAAGTATGT
    GTGAAAGATT ACTGTTTTGC TCGGGAAGAC CGCACAGTGG GAATGGCTGT CATGCAACTG
    CGAGATCTGG CACAAAGGGG TAGCTGTGCC TGTTGGCTCC CTCTTGGCCG CCGCATTCAT
    ATGGATGAGA CAGGACTGAC CATCTTGCGC ATACTATCAC AAAGAAACAA TGATGAGGTG
    GCCAAAGAGT TTGTCAAACT CAAATCTGAC ACCCGCTCTC CAGAAGAGGG GAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa48437
    Clone ID Related Accession (Same CDS sequence) XM_012954756.3
    Accession Version XM_012954756.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5043bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein unc-13 homolog A isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012954756.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCTTTGC TTTGCGTGGG AGTAAAAAAG GCCACGTTTG ATGGAGCACA AGAGAAGTTT 
    AATGCCTACG TAACCTTGAA GGTGCAAAAT GTGAAGAGCA CAACGATAGC GGTGCGGGGA
    AACCAGCCCT GCTGGGAACA GGATTTCATG TTTGAGATTA ATCGTTTAGA CCTTGGTCTA
    ACAGTGGAGG TCTGGAACAA AGGATTGATC TGGGACACCA TGGTGGGAAC AGTATGGATT
    CCTTTAAGGA CAATCCGACA ATCCAATGAG GAGGGTCCTG GGGAATGGCT GACACTTGAT
    TCACAGGTCA TAATGACAGA TAATGAGATT TGTGGTACCA AAGATCCAAC CTTCCACCGC
    ATCTTACTGG ATACCCGATT TGAACTTCCT CTAGATATAC CAGAAGAAGA AGCTCGATAC
    TGGGCAAAGA AACTTGAGCA ACTTAATGCA ATGAGGGACC AGGATGATTA TGTTTTTCAA
    GAAGAAATTC CGGAGAATCC TCTTCCCGTC CATGGATCTC AATGTTGTAA CTGGAATTAT
    TTCGGCTGGG GGGAGCAGCA CAATGAGGAT CCCGATAGTG CAGTGGATGA TCGGGATAGT
    GATTACCGCA GTGAAACCAG CAACAGTATC CCGCCCCCCT ACTACTCCAC CTCACAGCCT
    AATGCCTCTG TGCACCAGTA CCCTGTACGA CAGCAACACA GTTCTCATGG ATCTTACACT
    GATTCAGTGC ACAGCTATGA GATGGACTTC TCTGACCATC GTCCAATCAG CCCCACTGGA
    AGTAGTCGAT ATGCTTCATC AGGGGAGATT AGTCGTGGCT CCTCACAGCT CAGTGATGAC
    TTTGACCCAG ATGATGACAG CTTGCAAGGT TCAGAAATAG AGGAAGAAAG GGATAGAGAC
    TCTTATCACT CCTGTCATAG TTCTGTCAGC TGCCGCAAGG ACTCGCCAAA TTGGGAAGGA
    GAGGAGGAGC CTGAAGTTTA TAGTGAAGAT GAGGAAGAAT ATCCTGACAG TAGGGGGCAG
    CTCTCTGACC AGGAGCTGTA TGATGATGAC CAGCTTGAGG AAGAAGAGAA AAAAGGAAGA
    TTAGCATATG GGCAGGAGGA TTCCATCTAT CAGTCATTGG AGGCTGAGGA GAAGGAACCC
    CTGGATATTC CATTGGAGAA GGACAGGACA CCAGAAGCTG TGCCAGAGGA GGTAGTAGAG
    CCAACAGTAC CAGCAGTCCA GTCTCCCCAA GCTGCAGACA GTTACAGAGA AGAAGAACCT
    GGAATGTCCA GGGCCAAATC TAATTGGTTA AGAGCATTCA ACAAGGTGCG CCTGCAGCTG
    CAAGAGGCAC GAGGTGAAGG GACTGGGGAG ATGTCCAAAT CCCTCTGGTT TAAGGATGGG
    CCAGCTGGTG GTCTTATAAT TATAGACAGT ATGCCAGACA TACGAAAGAG GAAACCAATC
    CCGTTGGTCA GTGACCTGGC AATGTCACTG GTTCAGTCAC GGAAGGCTGG GATTACTTCT
    GCTCTTGCAA CAAGCACCTT AAACAATGAA GAGCTAAAAA ACCATGTTTA CAAAAAGACC
    CTGCAAGCTT TAATATACCC AATATCCAGC ACCACTCCCC ACAACTTTGA AGTTTGGACT
    GCCACAACTC CTACCTATTG TTATGAATGC GAAGGGCTTC TGTGGGGAAT CGCTCGGCAG
    GGCATGCGTT GCACAGAGTG TGGTGTCAAG TGCCATGAGA AGTGCCAGGA TCTGCTGAAT
    GCAGATTGTC TTCAAAGAGC TGCAGAGAAG AGCTCAAAAC ATGGTGCAGA AGACCGGACT
    CAGAATATTA TTATGGTCTT GAAGGATCGT ATGAAGATCC GGGAGAGGAA CAAGCCAGAA
    ATATTTGAGC TGATCCAGGA AGTATTTGGG GTATTAAAGG CTACACACGC TCAGCAGATA
    AAGGCTGTGA AACAGAGTGT GCTGGATGGT ACTTCCAAAT GGTCAGCAAA GATCAGCATA
    ACTGTTGTAT GTGCACAGGG TCTACAGGCC AAAGATAAGA CAGGCTCCAG TGACCCATAT
    GTTACTGTTC AAGTTGGGAA AACCAAGAAA AGAACCAAAA CAATCTATGG AAATCTTAAT
    CCTGTCTGGG AAGAGAATTT TCACTTTGAA TGCCACAACT CCTCAGATCG TATTAAAGTA
    CGCGTGTGGG ATGAAGATGA TGATATCAAA TCTCGTGTGA AGCAGAGATT TAAAAGAGAA
    TCAGATGACT TCCTTGGACA AACAATTATT GAAGTCAGAA CACTTAGCGG AGAGATGGAT
    GTGTGGTATA ACTTGGATAA GAGAACAGAT AAATCCGCTG TCTCTGGGGC AATCCGTCTT
    CATATAAGTG TGGAAATAAA AGGAGAGGAA AAGGTGGCTC CCTATCACGT GCAATACACA
    TGTCTACATG AGAACTTATT CCATTACCTG ACGGATGTGC AGAATAATGG TGTGGTCAAG
    ATTCCAGATG CAAAAGGAGA CGACGCCTGG AAAGTTTATT TTGATGAGAC AGCCCAGGAA
    ATTGTGGATG AATTTGCAAT GAGATATGGA GTGGAGTCCA TATATCAGGC CATGACGCAT
    TTTGCCTGCC TTTCATCTAA ATATATGTGT CCAGGAGTAC CAGCTGTAAT GAGTACTCTT
    CTTGCCAACA TTAATGCTTA CTATGCGCAC ACAACTGCAT CCACTAATGT GTCTGCCTCC
    GACCGATTCT CTGCCTCAAA CTTTGGGAAG GAGAGATTTG TAAAGCTGCT TGACCAGTTG
    CACAATTCCT TACGCATTGA CCTGTCCATG TATCGGAACA ACTTCCCTGC TAGCAGCCCT
    GAAAGACTCC AAGATCTCAA GTCCACTGTA GATTTACTAA CTAGCATAAC ATTTTTCCGG
    ATGAAGGTAC AAGAGCTTCA GAGCCCACCA CGGGCAAGCC AAGTTGTTAA GGACTGTGTG
    AAGGCATGCC TTAACTCAAC CTACGAATAT ATCTTTAACA ACTGCCATGA ACTTTATAGC
    CGAGAATATC AAAGTGACCC TGCTAAAAAA GGGGAGGTAC CCCCAGAAGA ACAGGGGCCA
    AGTATAAAGA ACTTAGATTT TTGGTCCAAA CTAATCACAT TGATTGTGTC TATCATAGAG
    GAAGATAAGA ACTCATATAC ACCTTGTCTA AACCAGTTTC CTCAGGAGCT AAATGTTGGT
    AAGATTAGTG CTGAGGTCAT GTGGAGTCTC TTTGCTCAAG ACATGAGATA TGCTATGGAA
    GAACACGACA AACACCGCCT GTGTAAGAGT GCAGACTACA TGAACTTGCA TTTTAAGGTC
    AAGTGGTTGT ACAATGAATA TGTAACTGAG CTACCATCTT TTAAGAGCAA GGTTCCAGAA
    TATCCCGCCT GGTTTGAGCC TTTTGTAATT CAGTGGCTGG ATGAAAATGA AGAGGTCTCC
    CGGGATTTTC TGCATGGAGC ACTTGAAAGA GATAAGAAGG ATGGGTTCCA GCAGACCTCT
    GAACATGCCC TGTTCTCCTG CTCTGTGGTG GATGTTTTCT CACAACTTAA CCAGAGCTTT
    GAGATCATTA AGAAGTTGGA GTGTCCAGAC CCACAAATTG TGGGGCATTA CATGCGTAGA
    TTTGCCAAGA CGATCAGTAA TGTTCTGCTG CAGTATGCTG AAATTATTTC CAAAGATTTT
    GCATCTCATT GCTCCAAAGA GAAGAAAGAA GAAAAAGTGC CCTGTATTCT TATGAACAAC
    ATCCAGCAAC TTCGAGTGCA ACTAGAAAAA ATGTTTGAAG CAATGGGAGG AAAGGAGCTG
    GATGCAGAAA CAAGTGACAT TCTTAAAGAG CTGCAGGTGA AACTCAACAA TGTCCTAGAT
    GAGCTAAGCA GAGTGTTTAC CACCAGTTTT CAGGCACAAA TTGAGGAGTG TGTGAAGCAG
    ATGGGTGACA TCCTGAGCCA GGTAAAAGGG ACCGGAAATG TACCAGCCAG TAACTGCAGC
    AGTGTGGCTC AGGATGCAGA TAATGTATTG CAGCCTATCC TGGATCCTCT GGACAGCAAC
    CTGACACTAT TTGCCAAGAT CTGTGAAAAG ACAGTCCTGA AGCGGGTACT AAAAGAACTC
    TGGAAGCTTG TGATGAATAC AATGGAGAAA ACGATTGTCC TTCCACCACT AACAGACCAA
    ACAATGATAG GGACGCTGCT GAGAAAACAT GGCAAGGGAA TAGAGAAGAG CAGGGTGAAG
    CTGCCGTACC ACGCAGATGG AACCCACCTT ATCATTAATG CAGCCAAGGA ACTGGGCCAG
    TTATCCAAAC TAAAGGATCA CATGGTGCGA GAGGAAGCCA AGAGCCTCAC CCCTAAGCAG
    TGTGCAGTGG TGGAGCTTGC ACTTGATACA ATTAAGCAAT ATTTCCATGC TGGAGGTGTT
    GGTCTCAAGA AGACATTCCT GGAGAAGAGT CCAGACCTAC AATCTCTACG CTATGCACTT
    TCTCTTTACA CACAAGCAAC CGATACGCTA ATCAAAACCT TCGTCCAGAC ACAGTCTGCT
    CAGGGATCTG GGGTGGATGA TTCTGTTGGG GAAGTTTCTA TCCATGTGGA GTTATTTACT
    CACCCAGGAA CAGGCGAGCA CAAGATCAGC GTAAAGGTTG TAGCGGCCAA TGACCTTAAA
    TGGCAGACGT CAGGGATTTT CCGTCCCTTT ATTGAGGTAA ACATGATTGG TCCGAATCTG
    AGTGACAAAA AAAGGAAATT TACCACCAAG TCCAAGAACA ACAGCTGGGC CCCAAAATAT
    AACGAGAGCT TCCAATTTAC CCTGGGTAAT GAGACTGGTC CAGAGAGTTA TGAGCTGCAA
    GTATGTGTGA AAGATTACTG TTTTGCTCGG GAAGACCGCA CAGTGGGAAT GGCTGTCATG
    CAACTGCGAG ATCTGGCACA AAGGGGTAGC TGTGCCTGTT GGCTCCCTCT TGGCCGCCGC
    ATTCATATGG ATGAGACAGG ACTGACCATC TTGCGCATAC TATCACAAAG AAACAATGAT
    GAGGTGGCCA AAGAGTTTGT CAAACTCAAA TCTGACACCC GCTCTCCAGA AGAGGGGAGC
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012810210.2
    CDS186..5228
    Translation

    Target ORF information:

    RefSeq Version XM_012954756.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis unc-13 homolog A (unc13a), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012954756.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCTTTGC TTTGCGTGGG AGTAAAAAAG GCCACGTTTG ATGGAGCACA AGAGAAGTTT 
    AATGCCTACG TAACCTTGAA GGTGCAAAAT GTGAAGAGCA CAACGATAGC GGTGCGGGGA
    AACCAGCCCT GCTGGGAACA GGATTTCATG TTTGAGATTA ATCGTTTAGA CCTTGGTCTA
    ACAGTGGAGG TCTGGAACAA AGGATTGATC TGGGACACCA TGGTGGGAAC AGTATGGATT
    CCTTTAAGGA CAATCCGACA ATCCAATGAG GAGGGTCCTG GGGAATGGCT GACACTTGAT
    TCACAGGTCA TAATGACAGA TAATGAGATT TGTGGTACCA AAGATCCAAC CTTCCACCGC
    ATCTTACTGG ATACCCGATT TGAACTTCCT CTAGATATAC CAGAAGAAGA AGCTCGATAC
    TGGGCAAAGA AACTTGAGCA ACTTAATGCA ATGAGGGACC AGGATGATTA TGTTTTTCAA
    GAAGAAATTC CGGAGAATCC TCTTCCCGTC CATGGATCTC AATGTTGTAA CTGGAATTAT
    TTCGGCTGGG GGGAGCAGCA CAATGAGGAT CCCGATAGTG CAGTGGATGA TCGGGATAGT
    GATTACCGCA GTGAAACCAG CAACAGTATC CCGCCCCCCT ACTACTCCAC CTCACAGCCT
    AATGCCTCTG TGCACCAGTA CCCTGTACGA CAGCAACACA GTTCTCATGG ATCTTACACT
    GATTCAGTGC ACAGCTATGA GATGGACTTC TCTGACCATC GTCCAATCAG CCCCACTGGA
    AGTAGTCGAT ATGCTTCATC AGGGGAGATT AGTCGTGGCT CCTCACAGCT CAGTGATGAC
    TTTGACCCAG ATGATGACAG CTTGCAAGGT TCAGAAATAG AGGAAGAAAG GGATAGAGAC
    TCTTATCACT CCTGTCATAG TTCTGTCAGC TGCCGCAAGG ACTCGCCAAA TTGGGAAGGA
    GAGGAGGAGC CTGAAGTTTA TAGTGAAGAT GAGGAAGAAT ATCCTGACAG TAGGGGGCAG
    CTCTCTGACC AGGAGCTGTA TGATGATGAC CAGCTTGAGG AAGAAGAGAA AAAAGGAAGA
    TTAGCATATG GGCAGGAGGA TTCCATCTAT CAGTCATTGG AGGCTGAGGA GAAGGAACCC
    CTGGATATTC CATTGGAGAA GGACAGGACA CCAGAAGCTG TGCCAGAGGA GGTAGTAGAG
    CCAACAGTAC CAGCAGTCCA GTCTCCCCAA GCTGCAGACA GTTACAGAGA AGAAGAACCT
    GGAATGTCCA GGGCCAAATC TAATTGGTTA AGAGCATTCA ACAAGGTGCG CCTGCAGCTG
    CAAGAGGCAC GAGGTGAAGG GACTGGGGAG ATGTCCAAAT CCCTCTGGTT TAAGGATGGG
    CCAGCTGGTG GTCTTATAAT TATAGACAGT ATGCCAGACA TACGAAAGAG GAAACCAATC
    CCGTTGGTCA GTGACCTGGC AATGTCACTG GTTCAGTCAC GGAAGGCTGG GATTACTTCT
    GCTCTTGCAA CAAGCACCTT AAACAATGAA GAGCTAAAAA ACCATGTTTA CAAAAAGACC
    CTGCAAGCTT TAATATACCC AATATCCAGC ACCACTCCCC ACAACTTTGA AGTTTGGACT
    GCCACAACTC CTACCTATTG TTATGAATGC GAAGGGCTTC TGTGGGGAAT CGCTCGGCAG
    GGCATGCGTT GCACAGAGTG TGGTGTCAAG TGCCATGAGA AGTGCCAGGA TCTGCTGAAT
    GCAGATTGTC TTCAAAGAGC TGCAGAGAAG AGCTCAAAAC ATGGTGCAGA AGACCGGACT
    CAGAATATTA TTATGGTCTT GAAGGATCGT ATGAAGATCC GGGAGAGGAA CAAGCCAGAA
    ATATTTGAGC TGATCCAGGA AGTATTTGGG GTATTAAAGG CTACACACGC TCAGCAGATA
    AAGGCTGTGA AACAGAGTGT GCTGGATGGT ACTTCCAAAT GGTCAGCAAA GATCAGCATA
    ACTGTTGTAT GTGCACAGGG TCTACAGGCC AAAGATAAGA CAGGCTCCAG TGACCCATAT
    GTTACTGTTC AAGTTGGGAA AACCAAGAAA AGAACCAAAA CAATCTATGG AAATCTTAAT
    CCTGTCTGGG AAGAGAATTT TCACTTTGAA TGCCACAACT CCTCAGATCG TATTAAAGTA
    CGCGTGTGGG ATGAAGATGA TGATATCAAA TCTCGTGTGA AGCAGAGATT TAAAAGAGAA
    TCAGATGACT TCCTTGGACA AACAATTATT GAAGTCAGAA CACTTAGCGG AGAGATGGAT
    GTGTGGTATA ACTTGGATAA GAGAACAGAT AAATCCGCTG TCTCTGGGGC AATCCGTCTT
    CATATAAGTG TGGAAATAAA AGGAGAGGAA AAGGTGGCTC CCTATCACGT GCAATACACA
    TGTCTACATG AGAACTTATT CCATTACCTG ACGGATGTGC AGAATAATGG TGTGGTCAAG
    ATTCCAGATG CAAAAGGAGA CGACGCCTGG AAAGTTTATT TTGATGAGAC AGCCCAGGAA
    ATTGTGGATG AATTTGCAAT GAGATATGGA GTGGAGTCCA TATATCAGGC CATGACGCAT
    TTTGCCTGCC TTTCATCTAA ATATATGTGT CCAGGAGTAC CAGCTGTAAT GAGTACTCTT
    CTTGCCAACA TTAATGCTTA CTATGCGCAC ACAACTGCAT CCACTAATGT GTCTGCCTCC
    GACCGATTCT CTGCCTCAAA CTTTGGGAAG GAGAGATTTG TAAAGCTGCT TGACCAGTTG
    CACAATTCCT TACGCATTGA CCTGTCCATG TATCGGAACA ACTTCCCTGC TAGCAGCCCT
    GAAAGACTCC AAGATCTCAA GTCCACTGTA GATTTACTAA CTAGCATAAC ATTTTTCCGG
    ATGAAGGTAC AAGAGCTTCA GAGCCCACCA CGGGCAAGCC AAGTTGTTAA GGACTGTGTG
    AAGGCATGCC TTAACTCAAC CTACGAATAT ATCTTTAACA ACTGCCATGA ACTTTATAGC
    CGAGAATATC AAAGTGACCC TGCTAAAAAA GGGGAGGTAC CCCCAGAAGA ACAGGGGCCA
    AGTATAAAGA ACTTAGATTT TTGGTCCAAA CTAATCACAT TGATTGTGTC TATCATAGAG
    GAAGATAAGA ACTCATATAC ACCTTGTCTA AACCAGTTTC CTCAGGAGCT AAATGTTGGT
    AAGATTAGTG CTGAGGTCAT GTGGAGTCTC TTTGCTCAAG ACATGAGATA TGCTATGGAA
    GAACACGACA AACACCGCCT GTGTAAGAGT GCAGACTACA TGAACTTGCA TTTTAAGGTC
    AAGTGGTTGT ACAATGAATA TGTAACTGAG CTACCATCTT TTAAGAGCAA GGTTCCAGAA
    TATCCCGCCT GGTTTGAGCC TTTTGTAATT CAGTGGCTGG ATGAAAATGA AGAGGTCTCC
    CGGGATTTTC TGCATGGAGC ACTTGAAAGA GATAAGAAGG ATGGGTTCCA GCAGACCTCT
    GAACATGCCC TGTTCTCCTG CTCTGTGGTG GATGTTTTCT CACAACTTAA CCAGAGCTTT
    GAGATCATTA AGAAGTTGGA GTGTCCAGAC CCACAAATTG TGGGGCATTA CATGCGTAGA
    TTTGCCAAGA CGATCAGTAA TGTTCTGCTG CAGTATGCTG AAATTATTTC CAAAGATTTT
    GCATCTCATT GCTCCAAAGA GAAGAAAGAA GAAAAAGTGC CCTGTATTCT TATGAACAAC
    ATCCAGCAAC TTCGAGTGCA ACTAGAAAAA ATGTTTGAAG CAATGGGAGG AAAGGAGCTG
    GATGCAGAAA CAAGTGACAT TCTTAAAGAG CTGCAGGTGA AACTCAACAA TGTCCTAGAT
    GAGCTAAGCA GAGTGTTTAC CACCAGTTTT CAGGCACAAA TTGAGGAGTG TGTGAAGCAG
    ATGGGTGACA TCCTGAGCCA GGTAAAAGGG ACCGGAAATG TACCAGCCAG TAACTGCAGC
    AGTGTGGCTC AGGATGCAGA TAATGTATTG CAGCCTATCC TGGATCCTCT GGACAGCAAC
    CTGACACTAT TTGCCAAGAT CTGTGAAAAG ACAGTCCTGA AGCGGGTACT AAAAGAACTC
    TGGAAGCTTG TGATGAATAC AATGGAGAAA ACGATTGTCC TTCCACCACT AACAGACCAA
    ACAATGATAG GGACGCTGCT GAGAAAACAT GGCAAGGGAA TAGAGAAGAG CAGGGTGAAG
    CTGCCGTACC ACGCAGATGG AACCCACCTT ATCATTAATG CAGCCAAGGA ACTGGGCCAG
    TTATCCAAAC TAAAGGATCA CATGGTGCGA GAGGAAGCCA AGAGCCTCAC CCCTAAGCAG
    TGTGCAGTGG TGGAGCTTGC ACTTGATACA ATTAAGCAAT ATTTCCATGC TGGAGGTGTT
    GGTCTCAAGA AGACATTCCT GGAGAAGAGT CCAGACCTAC AATCTCTACG CTATGCACTT
    TCTCTTTACA CACAAGCAAC CGATACGCTA ATCAAAACCT TCGTCCAGAC ACAGTCTGCT
    CAGGGATCTG GGGTGGATGA TTCTGTTGGG GAAGTTTCTA TCCATGTGGA GTTATTTACT
    CACCCAGGAA CAGGCGAGCA CAAGATCAGC GTAAAGGTTG TAGCGGCCAA TGACCTTAAA
    TGGCAGACGT CAGGGATTTT CCGTCCCTTT ATTGAGGTAA ACATGATTGG TCCGAATCTG
    AGTGACAAAA AAAGGAAATT TACCACCAAG TCCAAGAACA ACAGCTGGGC CCCAAAATAT
    AACGAGAGCT TCCAATTTAC CCTGGGTAAT GAGACTGGTC CAGAGAGTTA TGAGCTGCAA
    GTATGTGTGA AAGATTACTG TTTTGCTCGG GAAGACCGCA CAGTGGGAAT GGCTGTCATG
    CAACTGCGAG ATCTGGCACA AAGGGGTAGC TGTGCCTGTT GGCTCCCTCT TGGCCGCCGC
    ATTCATATGG ATGAGACAGG ACTGACCATC TTGCGCATAC TATCACAAAG AAACAATGAT
    GAGGTGGCCA AAGAGTTTGT CAAACTCAAA TCTGACACCC GCTCTCCAGA AGAGGGGAGC
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa35340
    Clone ID Related Accession (Same CDS sequence) XM_012954733.2
    Accession Version XM_012954733.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5226bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein unc-13 homolog A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012954733.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGTCTTTGC TTTGCGTGGG AGTAAAAAAG GCCACGTTTG ATGGAGCACA AGAGAAGTTT 
    AATGCCTACG TAACCTTGAA GGTGCAAAAT GTGAAGAGCA CAACGATAGC GGTGCGGGGA
    AACCAGCCCT GCTGGGAACA GGATTTCATG TTTGAGATTA ATCGTTTAGA CCTTGGTCTA
    ACAGTGGAGG TCTGGAACAA AGGATTGATC TGGGACACCA TGGTGGGAAC AGTATGGATT
    CCTTTAAGGA CAATCCGACA ATCCAATGAG GAGGGTCCTG GGGAATGGCT GACACTTGAT
    TCACAGGTCA TAATGACAGA TAATGAGATT TGTGGTACCA AAGATCCAAC CTTCCACCGC
    ATCTTACTGG ATACCCGATT TGAGCTTCCT CTAGATATAC CAGAAGAAGA AGCTCGATAC
    TGGGCAAAGA AACTCGAGCA ACTTAATGCA ATGAGGGACC AGGATGATTA TGTTTTTCAA
    GAAGAAATTC CGGAGAATCC TCTTCCTGTC CATGGATCTC AATGTTGTAA CTGGAATTAT
    TTCGGCTGGG GGGAGCAGCA CAATGAGGAT CCCGATAGTG CAGTGGATGA TCGGGATAGT
    GATTACCGCA GTGAAACCAG CAACAGTATC CCGCCCCCCT ACTACTCCAC CTCACAGCCT
    AATGCCTCTG TGCACCAGTA CCCTGTACGA CAGCAACACA GTTCTCATGG ATCTTACACT
    GATTCAGTGC ACAGCTATGA GATGGACTTC TCTGACCATC GTCCAATCAG GCGTTACGAT
    AGCGCAGACT CTGCCACTAA CGGGCGCAGT GATGTGGAGT CTGTCTCCGG TTCGAGCCGC
    CTCACCAGCC GGCAGCACTC TCTGGAGAGC CGGCAGTCCT TAGACCTGTC CGATAGCCCC
    ACTGGAAGTA GTCGATATGC TTCATCAGGG GAGATTAGTC GTGGCTCCTC ACAGCTCAGT
    GATGACTTTG ACCCAGATGA TGACAGCTTG CAAGGTTCAG AAATAGAGGA AGAAAGGGAT
    AGAGACTCTT ATCACTCCTG TCATAGTTCT GTCAGCTGCC GCAAGGACTC GCCAAATTGG
    GAAGGAGAGG AGGAGCGTGA AGTTTATAGT GAAGATGAGG AAGAATATCC TGACAGTAGG
    GGGCAGCTCT CTGACCAGGA GCTGTATGAT GATGACCAGC TTGAGGAAGA AGAGAAAAAA
    GGAAGATTAG CATATGGGCA GGAGGATTCC ATCTATCAGT CATTGGAGGC TGAGGAGAAG
    GAACCCCTGG ATATTCCATT GGAGAAGGAC AGGACACCAG AAGCTGTGCC AGAGGAGGTA
    GTAGAGCCAA CAGTACCAGC AGTCCAGTCT CCCCAAGCTG CAGACAGTTA CAGAGAAGAA
    GAACCTGGAA TGTCCAGGGC CAAATCTAAT TGGTTAAGAG CATTCAACAA GGTGCGCCTG
    CAGCTGCAAG AGGCACGAGG TGAAGGGACT GGGGAGATGT CCAAATCCCT CTGGTTTAAG
    GATGGGCCAG CTGGTGGTCT TATAATTATA GACAGTATGC CAGACATACG AAAGAGGAAA
    CCAATCCCGT TGGTCAGTGA CCTGGCAATG TCACTGGTTC AGTCACGGAA GGCTGGGATT
    ACTTCTGCTC TTGCAACAAG CACCTTAAAC AATGAAGAGC TAAAAAACCA TGTTTACAAA
    AAGACCCTGC AAGCTTTAAT ATACCCAATA TCCAGCACCA CTCCCCACAA CTTTGAAGTT
    TGGACTGCCA CAACTCCTAC CTATTGTTAT GAATGCGAAG GGCTTCTGTG GGGAATCGCT
    CGGCAGGGCA TGCGTTGCAC AGAGTGTGGT GTCAAGTGCC ATGAGAAGTG CCAGGATCTG
    CTGAATGCAG ATTGTCTTCA AAGAGCTGCA GAGAAGAGCT CAAAACATGG TGCAGAAGAC
    CGGACTCAGA ATATTATTAT GGTCTTGAAG GATCGTATGA AGATCCGGGA GAGGAACAAG
    CCAGAAATAT TTGAGCTGAT CCAGGAAGTA TTTGGGGTAT TAAAGGCTAC ACACGCTCAG
    CAGATAAAGG CTGTGAAACA GAGTGTGCTG GATGGTACTT CCAAATGGTC AGCAAAGATC
    AGCATAACTG TTGTATGTGC ACAGGGTCTA CAGGCCAAAG ATAAGACAGG CTCCAGTGAC
    CCATATGTTA CTGTTCAAGT TGGGAAAACC AAGAAAAGAA CCAAAACAAT CTATGGAAAT
    CTTAATCCTG TCTGGGAAGA GAATTTTCAC TTTGAATGCC ACAACTCCTC AGATCGTATT
    AAAGTACGCG TGTGGGATGA AGATGATGAT ATCAAATCTC GTGTGAAGCA GAGATTTAAA
    AGAGAATCAG ATGACTTCCT TGGACAAACA ATTATTGAAG TCAGAACACT TAGCGGAGAG
    ATGGATGTGT GGTATAACTT GGATAAGAGA ACAGATAAAT CCGCTGTCTC TGGGGCAATC
    CGTCTTCATA TAAGTGTGGA AATAAAAGGA GAGGAAAAGG TGGCTCCCTA TCACGTGCAA
    TACACATGTC TACATGAGAA CTTATTCCAT TACCTGACGG ATGTGCAGAA TAATGGTGTG
    GTCAAGATTC CAGATGCAAA AGGAGACGAC GCCTGGAAAG TTTATTTTGA TGAGACAGCC
    CAGGAAATTG TGGATGAATT TGCAATGAGA TATGGAGTGG AGTCCATATA CCAGGCCATG
    ACGCATTTTG CCTGCCTTTC ATCTAAATAT ATGTGTCCAG GAGTACCAGC TGTAATGAGT
    ACTCTTCTTG CCAACATTAA TGCTTACTAT GCGCACACAA CTGCATCTAC TAATGTGTCT
    GCCTCCGACC GATTCTCTGC CTCAAACTTT GGGAAGGAGA GATTTGTAAA GCTGCTTGAC
    CAGTTGCACA ATTCCTTACG CATTGACCTG TCCATGTATC GGAACAACTT CCCTGCTAGC
    AGCCCTGAAA GACTCCAAGA TCTCAAGTCC ACTGTAGATT TACTAACTAG CATAACATTT
    TTCCGGATGA AGGTACAAGA GCTTCAGAGC CCACCACGGG CAAGCCAAGT TGTTAAGGAC
    TGTGTGAAGG CATGCCTTAA CTCAACCTAC GAATATATCT TTAACAACTG CCATGAACTT
    TATAGCCGAG AATATCAAAG TGACCCTGCT AAAAAAGGGG AGGTACCCCC AGAAGAACAG
    GGGCCAAGTA TAAAGAACTT AGATTTTTGG TCCAAACTAA TCACATTGAT TGTGTCTATC
    ATAGAGGAAG ATAAGAACTC ATATACACCT TGTCTAAACC AGTTTCCTCA GGAGCTAAAT
    GTTGGTAAGA TTAGTGCTGA GGTCATGTGG AGTCTCTTTG CTCAAGACAT GAGATATGCT
    ATGGAAGAAC ACGACAAACA CCGCCTGTGT AAGAGTGCAG ACTACATGAA CTTGCATTTT
    AAGGTCAAGT GGTTGTACAA TGAATATGTA ACTGAGCTAC CATCTTTTAA GAGCAAGGTT
    CCAGAATATC CCGCCTGGTT TGAGCCTTTT GTAATTCAGT GGCTGGATGA AAATGAAGAG
    GTCTCCCGGG ATTTTCTGCA TGGAGCACTT GAAAGAGATA AGAAGGATGG GTTCCAGCAG
    ACCTCTGAAC ATGCCCTGTT CTCCTGCTCT GTGGTAGATG TTTTCTCACA ACTTAACCAG
    AGCTTTGAGA TCATTAAGAA GTTGGAGTGT CCAGACCCAC AAATTGTGGG GCATTACATG
    CGTAGATTTG CCAAGACGAT CAGTAATGTT CTGCTGCAGT ATGCTGAAAT TATTTCCAAA
    GATTTTGCAT CTCATTGCTC CAAAGAGAAG AAAGAAGAAA AAGTGCCCTG TATTCTTATG
    AACAATATCC AGCAACTTCG AGTGCAACTA GAAAAAATGT TTGAAGCAAT GGGAGGAAAG
    GAGCTGGATG CAGAAACAAG TGACATTCTT AAAGAGCTGC AGGTGAAACT CAACAATGTC
    CTAGATGAGC TAAGCAGAGT GTTTACCACC AGTTTTCAGG CACAAATTGA GGAGTGTGTG
    AAGCAGATGG GTGACATTCT GAGCCAGGTA AAAGGGACCG GAAATGTACC AGCCAGTAAC
    TGCAGCAGTG TGGCTCAGGA TGCAGATAAT GTATTGCAGC CTATCCTGGA TCCTCTGGAC
    AGCAACCTGA CACTATTTGC CAAGATCTGT GAAAAGACAG TCCTGAAGCG GGTACTAAAA
    GAACTCTGGA AGCTTGTGAT GAATACAATG GAGAAAACGA TTGTCCTTCC ACCACTAACA
    GACCAAACAA TGATAGGGAC GCTGCTGAGA AAACATGGCA AGGGAATAGA GAAGAGCAGG
    GTGAAGCTGC CGTACCACGC AGATGGAACC CACCTTATCA TTAATGCAGC CAAGGAACTG
    GGCCAGTTAT CCAAACTAAA GGATCACATG GTGCGAGAGG AAGCCAAGAG CCTCACCCCT
    AAGCAGTGTG CAGTGGTGGA GCTTGCACTT GATACAATTA AGCAATATTT CCATGCTGGA
    GGTGTTGGTC TCAAGAAGAC ATTCCTGGAG AAGAGTCCAG ACCTACAATC TCTACGCTAT
    GCACTTTCTC TTTACACACA AGCAACCGAT ACGCTAATCA AAACCTTCGT CCAGACACAG
    TCTGCTCAGG TTCATGGTGG TAAAGGTATT AGGTTTACAC TTAATGAAGA GACTTATCCA
    GAAAAGGGAT CTGGGGTGGA TGATTCTGTT GGGGAAGTTT CTATCCATGT GGAGTTATTT
    ACTCACCCAG GAACAGGCGA GCACAAGATC AGCGTAAAGG TTGTAGCGGC CAATGACCTT
    AAATGGCAGA CGTCAGGGAT TTTCCGTCCC TTTATTGAGG TAAACATGAT TGGTCCGAAT
    CTGAGTGACA AAAAAAGGAA ATTTACCACC AAGTCCAAGA ACAACAGCTG GGCCCCAAAA
    TATAACGAGA GCTTCCAATT TACCCTGGGT AATGAGACTG GTCCAGAGAG TTATGAGCTG
    CAAGTATGTG TGAAAGATTA CTGTTTTGCT CGGGAAGACC GCACAGTGGG AATGGCTGTC
    ATGCAACTGC GAGATCTGGC ACAAAGGGGT AGCTGTGCCT GTTGGCTCCC TCTTGGCCGC
    CGCATTCATA TGGATGAGAC AGGACTGACC ATCTTGCGCA TACTATCACA AAGAAACAAT
    GATGAGGTGG CCAAAGAGTT TGTCAAACTC AAATCTGACA CCCGCTCTCC AGAAGAGGGG
    AGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012810187.1
    CDS188..5413
    Translation

    Target ORF information:

    RefSeq Version XM_012954733.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis unc-13 homolog A (unc13a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012954733.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGTCTTTGC TTTGCGTGGG AGTAAAAAAG GCCACGTTTG ATGGAGCACA AGAGAAGTTT 
    AATGCCTACG TAACCTTGAA GGTGCAAAAT GTGAAGAGCA CAACGATAGC GGTGCGGGGA
    AACCAGCCCT GCTGGGAACA GGATTTCATG TTTGAGATTA ATCGTTTAGA CCTTGGTCTA
    ACAGTGGAGG TCTGGAACAA AGGATTGATC TGGGACACCA TGGTGGGAAC AGTATGGATT
    CCTTTAAGGA CAATCCGACA ATCCAATGAG GAGGGTCCTG GGGAATGGCT GACACTTGAT
    TCACAGGTCA TAATGACAGA TAATGAGATT TGTGGTACCA AAGATCCAAC CTTCCACCGC
    ATCTTACTGG ATACCCGATT TGAGCTTCCT CTAGATATAC CAGAAGAAGA AGCTCGATAC
    TGGGCAAAGA AACTCGAGCA ACTTAATGCA ATGAGGGACC AGGATGATTA TGTTTTTCAA
    GAAGAAATTC CGGAGAATCC TCTTCCTGTC CATGGATCTC AATGTTGTAA CTGGAATTAT
    TTCGGCTGGG GGGAGCAGCA CAATGAGGAT CCCGATAGTG CAGTGGATGA TCGGGATAGT
    GATTACCGCA GTGAAACCAG CAACAGTATC CCGCCCCCCT ACTACTCCAC CTCACAGCCT
    AATGCCTCTG TGCACCAGTA CCCTGTACGA CAGCAACACA GTTCTCATGG ATCTTACACT
    GATTCAGTGC ACAGCTATGA GATGGACTTC TCTGACCATC GTCCAATCAG GCGTTACGAT
    AGCGCAGACT CTGCCACTAA CGGGCGCAGT GATGTGGAGT CTGTCTCCGG TTCGAGCCGC
    CTCACCAGCC GGCAGCACTC TCTGGAGAGC CGGCAGTCCT TAGACCTGTC CGATAGCCCC
    ACTGGAAGTA GTCGATATGC TTCATCAGGG GAGATTAGTC GTGGCTCCTC ACAGCTCAGT
    GATGACTTTG ACCCAGATGA TGACAGCTTG CAAGGTTCAG AAATAGAGGA AGAAAGGGAT
    AGAGACTCTT ATCACTCCTG TCATAGTTCT GTCAGCTGCC GCAAGGACTC GCCAAATTGG
    GAAGGAGAGG AGGAGCGTGA AGTTTATAGT GAAGATGAGG AAGAATATCC TGACAGTAGG
    GGGCAGCTCT CTGACCAGGA GCTGTATGAT GATGACCAGC TTGAGGAAGA AGAGAAAAAA
    GGAAGATTAG CATATGGGCA GGAGGATTCC ATCTATCAGT CATTGGAGGC TGAGGAGAAG
    GAACCCCTGG ATATTCCATT GGAGAAGGAC AGGACACCAG AAGCTGTGCC AGAGGAGGTA
    GTAGAGCCAA CAGTACCAGC AGTCCAGTCT CCCCAAGCTG CAGACAGTTA CAGAGAAGAA
    GAACCTGGAA TGTCCAGGGC CAAATCTAAT TGGTTAAGAG CATTCAACAA GGTGCGCCTG
    CAGCTGCAAG AGGCACGAGG TGAAGGGACT GGGGAGATGT CCAAATCCCT CTGGTTTAAG
    GATGGGCCAG CTGGTGGTCT TATAATTATA GACAGTATGC CAGACATACG AAAGAGGAAA
    CCAATCCCGT TGGTCAGTGA CCTGGCAATG TCACTGGTTC AGTCACGGAA GGCTGGGATT
    ACTTCTGCTC TTGCAACAAG CACCTTAAAC AATGAAGAGC TAAAAAACCA TGTTTACAAA
    AAGACCCTGC AAGCTTTAAT ATACCCAATA TCCAGCACCA CTCCCCACAA CTTTGAAGTT
    TGGACTGCCA CAACTCCTAC CTATTGTTAT GAATGCGAAG GGCTTCTGTG GGGAATCGCT
    CGGCAGGGCA TGCGTTGCAC AGAGTGTGGT GTCAAGTGCC ATGAGAAGTG CCAGGATCTG
    CTGAATGCAG ATTGTCTTCA AAGAGCTGCA GAGAAGAGCT CAAAACATGG TGCAGAAGAC
    CGGACTCAGA ATATTATTAT GGTCTTGAAG GATCGTATGA AGATCCGGGA GAGGAACAAG
    CCAGAAATAT TTGAGCTGAT CCAGGAAGTA TTTGGGGTAT TAAAGGCTAC ACACGCTCAG
    CAGATAAAGG CTGTGAAACA GAGTGTGCTG GATGGTACTT CCAAATGGTC AGCAAAGATC
    AGCATAACTG TTGTATGTGC ACAGGGTCTA CAGGCCAAAG ATAAGACAGG CTCCAGTGAC
    CCATATGTTA CTGTTCAAGT TGGGAAAACC AAGAAAAGAA CCAAAACAAT CTATGGAAAT
    CTTAATCCTG TCTGGGAAGA GAATTTTCAC TTTGAATGCC ACAACTCCTC AGATCGTATT
    AAAGTACGCG TGTGGGATGA AGATGATGAT ATCAAATCTC GTGTGAAGCA GAGATTTAAA
    AGAGAATCAG ATGACTTCCT TGGACAAACA ATTATTGAAG TCAGAACACT TAGCGGAGAG
    ATGGATGTGT GGTATAACTT GGATAAGAGA ACAGATAAAT CCGCTGTCTC TGGGGCAATC
    CGTCTTCATA TAAGTGTGGA AATAAAAGGA GAGGAAAAGG TGGCTCCCTA TCACGTGCAA
    TACACATGTC TACATGAGAA CTTATTCCAT TACCTGACGG ATGTGCAGAA TAATGGTGTG
    GTCAAGATTC CAGATGCAAA AGGAGACGAC GCCTGGAAAG TTTATTTTGA TGAGACAGCC
    CAGGAAATTG TGGATGAATT TGCAATGAGA TATGGAGTGG AGTCCATATA CCAGGCCATG
    ACGCATTTTG CCTGCCTTTC ATCTAAATAT ATGTGTCCAG GAGTACCAGC TGTAATGAGT
    ACTCTTCTTG CCAACATTAA TGCTTACTAT GCGCACACAA CTGCATCTAC TAATGTGTCT
    GCCTCCGACC GATTCTCTGC CTCAAACTTT GGGAAGGAGA GATTTGTAAA GCTGCTTGAC
    CAGTTGCACA ATTCCTTACG CATTGACCTG TCCATGTATC GGAACAACTT CCCTGCTAGC
    AGCCCTGAAA GACTCCAAGA TCTCAAGTCC ACTGTAGATT TACTAACTAG CATAACATTT
    TTCCGGATGA AGGTACAAGA GCTTCAGAGC CCACCACGGG CAAGCCAAGT TGTTAAGGAC
    TGTGTGAAGG CATGCCTTAA CTCAACCTAC GAATATATCT TTAACAACTG CCATGAACTT
    TATAGCCGAG AATATCAAAG TGACCCTGCT AAAAAAGGGG AGGTACCCCC AGAAGAACAG
    GGGCCAAGTA TAAAGAACTT AGATTTTTGG TCCAAACTAA TCACATTGAT TGTGTCTATC
    ATAGAGGAAG ATAAGAACTC ATATACACCT TGTCTAAACC AGTTTCCTCA GGAGCTAAAT
    GTTGGTAAGA TTAGTGCTGA GGTCATGTGG AGTCTCTTTG CTCAAGACAT GAGATATGCT
    ATGGAAGAAC ACGACAAACA CCGCCTGTGT AAGAGTGCAG ACTACATGAA CTTGCATTTT
    AAGGTCAAGT GGTTGTACAA TGAATATGTA ACTGAGCTAC CATCTTTTAA GAGCAAGGTT
    CCAGAATATC CCGCCTGGTT TGAGCCTTTT GTAATTCAGT GGCTGGATGA AAATGAAGAG
    GTCTCCCGGG ATTTTCTGCA TGGAGCACTT GAAAGAGATA AGAAGGATGG GTTCCAGCAG
    ACCTCTGAAC ATGCCCTGTT CTCCTGCTCT GTGGTAGATG TTTTCTCACA ACTTAACCAG
    AGCTTTGAGA TCATTAAGAA GTTGGAGTGT CCAGACCCAC AAATTGTGGG GCATTACATG
    CGTAGATTTG CCAAGACGAT CAGTAATGTT CTGCTGCAGT ATGCTGAAAT TATTTCCAAA
    GATTTTGCAT CTCATTGCTC CAAAGAGAAG AAAGAAGAAA AAGTGCCCTG TATTCTTATG
    AACAATATCC AGCAACTTCG AGTGCAACTA GAAAAAATGT TTGAAGCAAT GGGAGGAAAG
    GAGCTGGATG CAGAAACAAG TGACATTCTT AAAGAGCTGC AGGTGAAACT CAACAATGTC
    CTAGATGAGC TAAGCAGAGT GTTTACCACC AGTTTTCAGG CACAAATTGA GGAGTGTGTG
    AAGCAGATGG GTGACATTCT GAGCCAGGTA AAAGGGACCG GAAATGTACC AGCCAGTAAC
    TGCAGCAGTG TGGCTCAGGA TGCAGATAAT GTATTGCAGC CTATCCTGGA TCCTCTGGAC
    AGCAACCTGA CACTATTTGC CAAGATCTGT GAAAAGACAG TCCTGAAGCG GGTACTAAAA
    GAACTCTGGA AGCTTGTGAT GAATACAATG GAGAAAACGA TTGTCCTTCC ACCACTAACA
    GACCAAACAA TGATAGGGAC GCTGCTGAGA AAACATGGCA AGGGAATAGA GAAGAGCAGG
    GTGAAGCTGC CGTACCACGC AGATGGAACC CACCTTATCA TTAATGCAGC CAAGGAACTG
    GGCCAGTTAT CCAAACTAAA GGATCACATG GTGCGAGAGG AAGCCAAGAG CCTCACCCCT
    AAGCAGTGTG CAGTGGTGGA GCTTGCACTT GATACAATTA AGCAATATTT CCATGCTGGA
    GGTGTTGGTC TCAAGAAGAC ATTCCTGGAG AAGAGTCCAG ACCTACAATC TCTACGCTAT
    GCACTTTCTC TTTACACACA AGCAACCGAT ACGCTAATCA AAACCTTCGT CCAGACACAG
    TCTGCTCAGG TTCATGGTGG TAAAGGTATT AGGTTTACAC TTAATGAAGA GACTTATCCA
    GAAAAGGGAT CTGGGGTGGA TGATTCTGTT GGGGAAGTTT CTATCCATGT GGAGTTATTT
    ACTCACCCAG GAACAGGCGA GCACAAGATC AGCGTAAAGG TTGTAGCGGC CAATGACCTT
    AAATGGCAGA CGTCAGGGAT TTTCCGTCCC TTTATTGAGG TAAACATGAT TGGTCCGAAT
    CTGAGTGACA AAAAAAGGAA ATTTACCACC AAGTCCAAGA ACAACAGCTG GGCCCCAAAA
    TATAACGAGA GCTTCCAATT TACCCTGGGT AATGAGACTG GTCCAGAGAG TTATGAGCTG
    CAAGTATGTG TGAAAGATTA CTGTTTTGCT CGGGAAGACC GCACAGTGGG AATGGCTGTC
    ATGCAACTGC GAGATCTGGC ACAAAGGGGT AGCTGTGCCT GTTGGCTCCC TCTTGGCCGC
    CGCATTCATA TGGATGAGAC AGGACTGACC ATCTTGCGCA TACTATCACA AAGAAACAAT
    GATGAGGTGG CCAAAGAGTT TGTCAAACTC AAATCTGACA CCCGCTCTCC AGAAGAGGGG
    AGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa35345
    Clone ID Related Accession (Same CDS sequence) XM_012954752.2
    Accession Version XM_012954752.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5100bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein unc-13 homolog A isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012954752.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCTTTGC TTTGCGTGGG AGTAAAAAAG GCCACGTTTG ATGGAGCACA AGAGAAGTTT 
    AATGCCTACG TAACCTTGAA GGTGCAAAAT GTGAAGAGCA CAACGATAGC GGTGCGGGGA
    AACCAGCCCT GCTGGGAACA GGATTTCATG TTTGAGATTA ATCGTTTAGA CCTTGGTCTA
    ACAGTGGAGG TCTGGAACAA AGGATTGATC TGGGACACCA TGGTGGGAAC AGTATGGATT
    CCTTTAAGGA CAATCCGACA ATCCAATGAG GAGGGTCCTG GGGAATGGCT GACACTTGAT
    TCACAGGTCA TAATGACAGA TAATGAGATT TGTGGTACCA AAGATCCAAC CTTCCACCGC
    ATCTTACTGG ATACCCGATT TGAGCTTCCT CTAGATATAC CAGAAGAAGA AGCTCGATAC
    TGGGCAAAGA AACTCGAGCA ACTTAATGCA ATGAGGGACC AGGATGATTA TGTTTTTCAA
    GAAGAAATTC CGGAGAATCC TCTTCCTGTC CATGGATCTC AATGTTGTAA CTGGAATTAT
    TTCGGCTGGG GGGAGCAGCA CAATGAGGAT CCCGATAGTG CAGTGGATGA TCGGGATAGT
    GATTACCGCA GTGAAACCAG CAACAGTATC CCGCCCCCCT ACTACTCCAC CTCACAGCCT
    AATGCCTCTG TGCACCAGTA CCCTGTACGA CAGCAACACA GTTCTCATGG ATCTTACACT
    GATTCAGTGC ACAGCTATGA GATGGACTTC TCTGACCATC GTCCAATCAG CCCCACTGGA
    AGTAGTCGAT ATGCTTCATC AGGGGAGATT AGTCGTGGCT CCTCACAGCT CAGTGATGAC
    TTTGACCCAG ATGATGACAG CTTGCAAGGT TCAGAAATAG AGGAAGAAAG GGATAGAGAC
    TCTTATCACT CCTGTCATAG TTCTGTCAGC TGCCGCAAGG ACTCGCCAAA TTGGGAAGGA
    GAGGAGGAGC GTGAAGTTTA TAGTGAAGAT GAGGAAGAAT ATCCTGACAG TAGGGGGCAG
    CTCTCTGACC AGGAGCTGTA TGATGATGAC CAGCTTGAGG AAGAAGAGAA AAAAGGAAGA
    TTAGCATATG GGCAGGAGGA TTCCATCTAT CAGTCATTGG AGGCTGAGGA GAAGGAACCC
    CTGGATATTC CATTGGAGAA GGACAGGACA CCAGAAGCTG TGCCAGAGGA GGTAGTAGAG
    CCAACAGTAC CAGCAGTCCA GTCTCCCCAA GCTGCAGACA GTTACAGAGA AGAAGAACCT
    GGAATGTCCA GGGCCAAATC TAATTGGTTA AGAGCATTCA ACAAGGTGCG CCTGCAGCTG
    CAAGAGGCAC GAGGTGAAGG GACTGGGGAG ATGTCCAAAT CCCTCTGGTT TAAGGATGGG
    CCAGCTGGTG GTCTTATAAT TATAGACAGT ATGCCAGACA TACGAAAGAG GAAACCAATC
    CCGTTGGTCA GTGACCTGGC AATGTCACTG GTTCAGTCAC GGAAGGCTGG GATTACTTCT
    GCTCTTGCAA CAAGCACCTT AAACAATGAA GAGCTAAAAA ACCATGTTTA CAAAAAGACC
    CTGCAAGCTT TAATATACCC AATATCCAGC ACCACTCCCC ACAACTTTGA AGTTTGGACT
    GCCACAACTC CTACCTATTG TTATGAATGC GAAGGGCTTC TGTGGGGAAT CGCTCGGCAG
    GGCATGCGTT GCACAGAGTG TGGTGTCAAG TGCCATGAGA AGTGCCAGGA TCTGCTGAAT
    GCAGATTGTC TTCAAAGAGC TGCAGAGAAG AGCTCAAAAC ATGGTGCAGA AGACCGGACT
    CAGAATATTA TTATGGTCTT GAAGGATCGT ATGAAGATCC GGGAGAGGAA CAAGCCAGAA
    ATATTTGAGC TGATCCAGGA AGTATTTGGG GTATTAAAGG CTACACACGC TCAGCAGATA
    AAGGCTGTGA AACAGAGTGT GCTGGATGGT ACTTCCAAAT GGTCAGCAAA GATCAGCATA
    ACTGTTGTAT GTGCACAGGG TCTACAGGCC AAAGATAAGA CAGGCTCCAG TGACCCATAT
    GTTACTGTTC AAGTTGGGAA AACCAAGAAA AGAACCAAAA CAATCTATGG AAATCTTAAT
    CCTGTCTGGG AAGAGAATTT TCACTTTGAA TGCCACAACT CCTCAGATCG TATTAAAGTA
    CGCGTGTGGG ATGAAGATGA TGATATCAAA TCTCGTGTGA AGCAGAGATT TAAAAGAGAA
    TCAGATGACT TCCTTGGACA AACAATTATT GAAGTCAGAA CACTTAGCGG AGAGATGGAT
    GTGTGGTATA ACTTGGATAA GAGAACAGAT AAATCCGCTG TCTCTGGGGC AATCCGTCTT
    CATATAAGTG TGGAAATAAA AGGAGAGGAA AAGGTGGCTC CCTATCACGT GCAATACACA
    TGTCTACATG AGAACTTATT CCATTACCTG ACGGATGTGC AGAATAATGG TGTGGTCAAG
    ATTCCAGATG CAAAAGGAGA CGACGCCTGG AAAGTTTATT TTGATGAGAC AGCCCAGGAA
    ATTGTGGATG AATTTGCAAT GAGATATGGA GTGGAGTCCA TATACCAGGC CATGACGCAT
    TTTGCCTGCC TTTCATCTAA ATATATGTGT CCAGGAGTAC CAGCTGTAAT GAGTACTCTT
    CTTGCCAACA TTAATGCTTA CTATGCGCAC ACAACTGCAT CTACTAATGT GTCTGCCTCC
    GACCGATTCT CTGCCTCAAA CTTTGGGAAG GAGAGATTTG TAAAGCTGCT TGACCAGTTG
    CACAATTCCT TACGCATTGA CCTGTCCATG TATCGGAACA ACTTCCCTGC TAGCAGCCCT
    GAAAGACTCC AAGATCTCAA GTCCACTGTA GATTTACTAA CTAGCATAAC ATTTTTCCGG
    ATGAAGGTAC AAGAGCTTCA GAGCCCACCA CGGGCAAGCC AAGTTGTTAA GGACTGTGTG
    AAGGCATGCC TTAACTCAAC CTACGAATAT ATCTTTAACA ACTGCCATGA ACTTTATAGC
    CGAGAATATC AAAGTGACCC TGCTAAAAAA GGGGAGGTAC CCCCAGAAGA ACAGGGGCCA
    AGTATAAAGA ACTTAGATTT TTGGTCCAAA CTAATCACAT TGATTGTGTC TATCATAGAG
    GAAGATAAGA ACTCATATAC ACCTTGTCTA AACCAGTTTC CTCAGGAGCT AAATGTTGGT
    AAGATTAGTG CTGAGGTCAT GTGGAGTCTC TTTGCTCAAG ACATGAGATA TGCTATGGAA
    GAACACGACA AACACCGCCT GTGTAAGAGT GCAGACTACA TGAACTTGCA TTTTAAGGTC
    AAGTGGTTGT ACAATGAATA TGTAACTGAG CTACCATCTT TTAAGAGCAA GGTTCCAGAA
    TATCCCGCCT GGTTTGAGCC TTTTGTAATT CAGTGGCTGG ATGAAAATGA AGAGGTCTCC
    CGGGATTTTC TGCATGGAGC ACTTGAAAGA GATAAGAAGG ATGGGTTCCA GCAGACCTCT
    GAACATGCCC TGTTCTCCTG CTCTGTGGTA GATGTTTTCT CACAACTTAA CCAGAGCTTT
    GAGATCATTA AGAAGTTGGA GTGTCCAGAC CCACAAATTG TGGGGCATTA CATGCGTAGA
    TTTGCCAAGA CGATCAGTAA TGTTCTGCTG CAGTATGCTG AAATTATTTC CAAAGATTTT
    GCATCTCATT GCTCCAAAGA GAAGAAAGAA GAAAAAGTGC CCTGTATTCT TATGAACAAT
    ATCCAGCAAC TTCGAGTGCA ACTAGAAAAA ATGTTTGAAG CAATGGGAGG AAAGGAGCTG
    GATGCAGAAA CAAGTGACAT TCTTAAAGAG CTGCAGGTGA AACTCAACAA TGTCCTAGAT
    GAGCTAAGCA GAGTGTTTAC CACCAGTTTT CAGGCACAAA TTGAGGAGTG TGTGAAGCAG
    ATGGGTGACA TTCTGAGCCA GGTAAAAGGG ACCGGAAATG TACCAGCCAG TAACTGCAGC
    AGTGTGGCTC AGGATGCAGA TAATGTATTG CAGCCTATCC TGGATCCTCT GGACAGCAAC
    CTGACACTAT TTGCCAAGAT CTGTGAAAAG ACAGTCCTGA AGCGGGTACT AAAAGAACTC
    TGGAAGCTTG TGATGAATAC AATGGAGAAA ACGATTGTCC TTCCACCACT AACAGACCAA
    ACAATGATAG GGACGCTGCT GAGAAAACAT GGCAAGGGAA TAGAGAAGAG CAGGGTGAAG
    CTGCCGTACC ACGCAGATGG AACCCACCTT ATCATTAATG CAGCCAAGGA ACTGGGCCAG
    TTATCCAAAC TAAAGGATCA CATGGTGCGA GAGGAAGCCA AGAGCCTCAC CCCTAAGCAG
    TGTGCAGTGG TGGAGCTTGC ACTTGATACA ATTAAGCAAT ATTTCCATGC TGGAGGTGTT
    GGTCTCAAGA AGACATTCCT GGAGAAGAGT CCAGACCTAC AATCTCTACG CTATGCACTT
    TCTCTTTACA CACAAGCAAC CGATACGCTA ATCAAAACCT TCGTCCAGAC ACAGTCTGCT
    CAGGTTCATG GTGGTAAAGG TATTAGGTTT ACACTTAATG AAGAGACTTA TCCAGAAAAG
    GGATCTGGGG TGGATGATTC TGTTGGGGAA GTTTCTATCC ATGTGGAGTT ATTTACTCAC
    CCAGGAACAG GCGAGCACAA GATCAGCGTA AAGGTTGTAG CGGCCAATGA CCTTAAATGG
    CAGACGTCAG GGATTTTCCG TCCCTTTATT GAGGTAAACA TGATTGGTCC GAATCTGAGT
    GACAAAAAAA GGAAATTTAC CACCAAGTCC AAGAACAACA GCTGGGCCCC AAAATATAAC
    GAGAGCTTCC AATTTACCCT GGGTAATGAG ACTGGTCCAG AGAGTTATGA GCTGCAAGTA
    TGTGTGAAAG ATTACTGTTT TGCTCGGGAA GACCGCACAG TGGGAATGGC TGTCATGCAA
    CTGCGAGATC TGGCACAAAG GGGTAGCTGT GCCTGTTGGC TCCCTCTTGG CCGCCGCATT
    CATATGGATG AGACAGGACT GACCATCTTG CGCATACTAT CACAAAGAAA CAATGATGAG
    GTGGCCAAAG AGTTTGTCAA ACTCAAATCT GACACCCGCT CTCCAGAAGA GGGGAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012810206.1
    CDS188..5287
    Translation

    Target ORF information:

    RefSeq Version XM_012954752.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis unc-13 homolog A (unc13a), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012954752.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCTTTGC TTTGCGTGGG AGTAAAAAAG GCCACGTTTG ATGGAGCACA AGAGAAGTTT 
    AATGCCTACG TAACCTTGAA GGTGCAAAAT GTGAAGAGCA CAACGATAGC GGTGCGGGGA
    AACCAGCCCT GCTGGGAACA GGATTTCATG TTTGAGATTA ATCGTTTAGA CCTTGGTCTA
    ACAGTGGAGG TCTGGAACAA AGGATTGATC TGGGACACCA TGGTGGGAAC AGTATGGATT
    CCTTTAAGGA CAATCCGACA ATCCAATGAG GAGGGTCCTG GGGAATGGCT GACACTTGAT
    TCACAGGTCA TAATGACAGA TAATGAGATT TGTGGTACCA AAGATCCAAC CTTCCACCGC
    ATCTTACTGG ATACCCGATT TGAGCTTCCT CTAGATATAC CAGAAGAAGA AGCTCGATAC
    TGGGCAAAGA AACTCGAGCA ACTTAATGCA ATGAGGGACC AGGATGATTA TGTTTTTCAA
    GAAGAAATTC CGGAGAATCC TCTTCCTGTC CATGGATCTC AATGTTGTAA CTGGAATTAT
    TTCGGCTGGG GGGAGCAGCA CAATGAGGAT CCCGATAGTG CAGTGGATGA TCGGGATAGT
    GATTACCGCA GTGAAACCAG CAACAGTATC CCGCCCCCCT ACTACTCCAC CTCACAGCCT
    AATGCCTCTG TGCACCAGTA CCCTGTACGA CAGCAACACA GTTCTCATGG ATCTTACACT
    GATTCAGTGC ACAGCTATGA GATGGACTTC TCTGACCATC GTCCAATCAG CCCCACTGGA
    AGTAGTCGAT ATGCTTCATC AGGGGAGATT AGTCGTGGCT CCTCACAGCT CAGTGATGAC
    TTTGACCCAG ATGATGACAG CTTGCAAGGT TCAGAAATAG AGGAAGAAAG GGATAGAGAC
    TCTTATCACT CCTGTCATAG TTCTGTCAGC TGCCGCAAGG ACTCGCCAAA TTGGGAAGGA
    GAGGAGGAGC GTGAAGTTTA TAGTGAAGAT GAGGAAGAAT ATCCTGACAG TAGGGGGCAG
    CTCTCTGACC AGGAGCTGTA TGATGATGAC CAGCTTGAGG AAGAAGAGAA AAAAGGAAGA
    TTAGCATATG GGCAGGAGGA TTCCATCTAT CAGTCATTGG AGGCTGAGGA GAAGGAACCC
    CTGGATATTC CATTGGAGAA GGACAGGACA CCAGAAGCTG TGCCAGAGGA GGTAGTAGAG
    CCAACAGTAC CAGCAGTCCA GTCTCCCCAA GCTGCAGACA GTTACAGAGA AGAAGAACCT
    GGAATGTCCA GGGCCAAATC TAATTGGTTA AGAGCATTCA ACAAGGTGCG CCTGCAGCTG
    CAAGAGGCAC GAGGTGAAGG GACTGGGGAG ATGTCCAAAT CCCTCTGGTT TAAGGATGGG
    CCAGCTGGTG GTCTTATAAT TATAGACAGT ATGCCAGACA TACGAAAGAG GAAACCAATC
    CCGTTGGTCA GTGACCTGGC AATGTCACTG GTTCAGTCAC GGAAGGCTGG GATTACTTCT
    GCTCTTGCAA CAAGCACCTT AAACAATGAA GAGCTAAAAA ACCATGTTTA CAAAAAGACC
    CTGCAAGCTT TAATATACCC AATATCCAGC ACCACTCCCC ACAACTTTGA AGTTTGGACT
    GCCACAACTC CTACCTATTG TTATGAATGC GAAGGGCTTC TGTGGGGAAT CGCTCGGCAG
    GGCATGCGTT GCACAGAGTG TGGTGTCAAG TGCCATGAGA AGTGCCAGGA TCTGCTGAAT
    GCAGATTGTC TTCAAAGAGC TGCAGAGAAG AGCTCAAAAC ATGGTGCAGA AGACCGGACT
    CAGAATATTA TTATGGTCTT GAAGGATCGT ATGAAGATCC GGGAGAGGAA CAAGCCAGAA
    ATATTTGAGC TGATCCAGGA AGTATTTGGG GTATTAAAGG CTACACACGC TCAGCAGATA
    AAGGCTGTGA AACAGAGTGT GCTGGATGGT ACTTCCAAAT GGTCAGCAAA GATCAGCATA
    ACTGTTGTAT GTGCACAGGG TCTACAGGCC AAAGATAAGA CAGGCTCCAG TGACCCATAT
    GTTACTGTTC AAGTTGGGAA AACCAAGAAA AGAACCAAAA CAATCTATGG AAATCTTAAT
    CCTGTCTGGG AAGAGAATTT TCACTTTGAA TGCCACAACT CCTCAGATCG TATTAAAGTA
    CGCGTGTGGG ATGAAGATGA TGATATCAAA TCTCGTGTGA AGCAGAGATT TAAAAGAGAA
    TCAGATGACT TCCTTGGACA AACAATTATT GAAGTCAGAA CACTTAGCGG AGAGATGGAT
    GTGTGGTATA ACTTGGATAA GAGAACAGAT AAATCCGCTG TCTCTGGGGC AATCCGTCTT
    CATATAAGTG TGGAAATAAA AGGAGAGGAA AAGGTGGCTC CCTATCACGT GCAATACACA
    TGTCTACATG AGAACTTATT CCATTACCTG ACGGATGTGC AGAATAATGG TGTGGTCAAG
    ATTCCAGATG CAAAAGGAGA CGACGCCTGG AAAGTTTATT TTGATGAGAC AGCCCAGGAA
    ATTGTGGATG AATTTGCAAT GAGATATGGA GTGGAGTCCA TATACCAGGC CATGACGCAT
    TTTGCCTGCC TTTCATCTAA ATATATGTGT CCAGGAGTAC CAGCTGTAAT GAGTACTCTT
    CTTGCCAACA TTAATGCTTA CTATGCGCAC ACAACTGCAT CTACTAATGT GTCTGCCTCC
    GACCGATTCT CTGCCTCAAA CTTTGGGAAG GAGAGATTTG TAAAGCTGCT TGACCAGTTG
    CACAATTCCT TACGCATTGA CCTGTCCATG TATCGGAACA ACTTCCCTGC TAGCAGCCCT
    GAAAGACTCC AAGATCTCAA GTCCACTGTA GATTTACTAA CTAGCATAAC ATTTTTCCGG
    ATGAAGGTAC AAGAGCTTCA GAGCCCACCA CGGGCAAGCC AAGTTGTTAA GGACTGTGTG
    AAGGCATGCC TTAACTCAAC CTACGAATAT ATCTTTAACA ACTGCCATGA ACTTTATAGC
    CGAGAATATC AAAGTGACCC TGCTAAAAAA GGGGAGGTAC CCCCAGAAGA ACAGGGGCCA
    AGTATAAAGA ACTTAGATTT TTGGTCCAAA CTAATCACAT TGATTGTGTC TATCATAGAG
    GAAGATAAGA ACTCATATAC ACCTTGTCTA AACCAGTTTC CTCAGGAGCT AAATGTTGGT
    AAGATTAGTG CTGAGGTCAT GTGGAGTCTC TTTGCTCAAG ACATGAGATA TGCTATGGAA
    GAACACGACA AACACCGCCT GTGTAAGAGT GCAGACTACA TGAACTTGCA TTTTAAGGTC
    AAGTGGTTGT ACAATGAATA TGTAACTGAG CTACCATCTT TTAAGAGCAA GGTTCCAGAA
    TATCCCGCCT GGTTTGAGCC TTTTGTAATT CAGTGGCTGG ATGAAAATGA AGAGGTCTCC
    CGGGATTTTC TGCATGGAGC ACTTGAAAGA GATAAGAAGG ATGGGTTCCA GCAGACCTCT
    GAACATGCCC TGTTCTCCTG CTCTGTGGTA GATGTTTTCT CACAACTTAA CCAGAGCTTT
    GAGATCATTA AGAAGTTGGA GTGTCCAGAC CCACAAATTG TGGGGCATTA CATGCGTAGA
    TTTGCCAAGA CGATCAGTAA TGTTCTGCTG CAGTATGCTG AAATTATTTC CAAAGATTTT
    GCATCTCATT GCTCCAAAGA GAAGAAAGAA GAAAAAGTGC CCTGTATTCT TATGAACAAT
    ATCCAGCAAC TTCGAGTGCA ACTAGAAAAA ATGTTTGAAG CAATGGGAGG AAAGGAGCTG
    GATGCAGAAA CAAGTGACAT TCTTAAAGAG CTGCAGGTGA AACTCAACAA TGTCCTAGAT
    GAGCTAAGCA GAGTGTTTAC CACCAGTTTT CAGGCACAAA TTGAGGAGTG TGTGAAGCAG
    ATGGGTGACA TTCTGAGCCA GGTAAAAGGG ACCGGAAATG TACCAGCCAG TAACTGCAGC
    AGTGTGGCTC AGGATGCAGA TAATGTATTG CAGCCTATCC TGGATCCTCT GGACAGCAAC
    CTGACACTAT TTGCCAAGAT CTGTGAAAAG ACAGTCCTGA AGCGGGTACT AAAAGAACTC
    TGGAAGCTTG TGATGAATAC AATGGAGAAA ACGATTGTCC TTCCACCACT AACAGACCAA
    ACAATGATAG GGACGCTGCT GAGAAAACAT GGCAAGGGAA TAGAGAAGAG CAGGGTGAAG
    CTGCCGTACC ACGCAGATGG AACCCACCTT ATCATTAATG CAGCCAAGGA ACTGGGCCAG
    TTATCCAAAC TAAAGGATCA CATGGTGCGA GAGGAAGCCA AGAGCCTCAC CCCTAAGCAG
    TGTGCAGTGG TGGAGCTTGC ACTTGATACA ATTAAGCAAT ATTTCCATGC TGGAGGTGTT
    GGTCTCAAGA AGACATTCCT GGAGAAGAGT CCAGACCTAC AATCTCTACG CTATGCACTT
    TCTCTTTACA CACAAGCAAC CGATACGCTA ATCAAAACCT TCGTCCAGAC ACAGTCTGCT
    CAGGTTCATG GTGGTAAAGG TATTAGGTTT ACACTTAATG AAGAGACTTA TCCAGAAAAG
    GGATCTGGGG TGGATGATTC TGTTGGGGAA GTTTCTATCC ATGTGGAGTT ATTTACTCAC
    CCAGGAACAG GCGAGCACAA GATCAGCGTA AAGGTTGTAG CGGCCAATGA CCTTAAATGG
    CAGACGTCAG GGATTTTCCG TCCCTTTATT GAGGTAAACA TGATTGGTCC GAATCTGAGT
    GACAAAAAAA GGAAATTTAC CACCAAGTCC AAGAACAACA GCTGGGCCCC AAAATATAAC
    GAGAGCTTCC AATTTACCCT GGGTAATGAG ACTGGTCCAG AGAGTTATGA GCTGCAAGTA
    TGTGTGAAAG ATTACTGTTT TGCTCGGGAA GACCGCACAG TGGGAATGGC TGTCATGCAA
    CTGCGAGATC TGGCACAAAG GGGTAGCTGT GCCTGTTGGC TCCCTCTTGG CCGCCGCATT
    CATATGGATG AGACAGGACT GACCATCTTG CGCATACTAT CACAAAGAAA CAATGATGAG
    GTGGCCAAAG AGTTTGTCAA ACTCAAATCT GACACCCGCT CTCCAGAAGA GGGGAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa35346
    Clone ID Related Accession (Same CDS sequence) XM_012954756.2
    Accession Version XM_012954756.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5043bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein unc-13 homolog A isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012954756.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCTTTGC TTTGCGTGGG AGTAAAAAAG GCCACGTTTG ATGGAGCACA AGAGAAGTTT 
    AATGCCTACG TAACCTTGAA GGTGCAAAAT GTGAAGAGCA CAACGATAGC GGTGCGGGGA
    AACCAGCCCT GCTGGGAACA GGATTTCATG TTTGAGATTA ATCGTTTAGA CCTTGGTCTA
    ACAGTGGAGG TCTGGAACAA AGGATTGATC TGGGACACCA TGGTGGGAAC AGTATGGATT
    CCTTTAAGGA CAATCCGACA ATCCAATGAG GAGGGTCCTG GGGAATGGCT GACACTTGAT
    TCACAGGTCA TAATGACAGA TAATGAGATT TGTGGTACCA AAGATCCAAC CTTCCACCGC
    ATCTTACTGG ATACCCGATT TGAGCTTCCT CTAGATATAC CAGAAGAAGA AGCTCGATAC
    TGGGCAAAGA AACTCGAGCA ACTTAATGCA ATGAGGGACC AGGATGATTA TGTTTTTCAA
    GAAGAAATTC CGGAGAATCC TCTTCCTGTC CATGGATCTC AATGTTGTAA CTGGAATTAT
    TTCGGCTGGG GGGAGCAGCA CAATGAGGAT CCCGATAGTG CAGTGGATGA TCGGGATAGT
    GATTACCGCA GTGAAACCAG CAACAGTATC CCGCCCCCCT ACTACTCCAC CTCACAGCCT
    AATGCCTCTG TGCACCAGTA CCCTGTACGA CAGCAACACA GTTCTCATGG ATCTTACACT
    GATTCAGTGC ACAGCTATGA GATGGACTTC TCTGACCATC GTCCAATCAG CCCCACTGGA
    AGTAGTCGAT ATGCTTCATC AGGGGAGATT AGTCGTGGCT CCTCACAGCT CAGTGATGAC
    TTTGACCCAG ATGATGACAG CTTGCAAGGT TCAGAAATAG AGGAAGAAAG GGATAGAGAC
    TCTTATCACT CCTGTCATAG TTCTGTCAGC TGCCGCAAGG ACTCGCCAAA TTGGGAAGGA
    GAGGAGGAGC GTGAAGTTTA TAGTGAAGAT GAGGAAGAAT ATCCTGACAG TAGGGGGCAG
    CTCTCTGACC AGGAGCTGTA TGATGATGAC CAGCTTGAGG AAGAAGAGAA AAAAGGAAGA
    TTAGCATATG GGCAGGAGGA TTCCATCTAT CAGTCATTGG AGGCTGAGGA GAAGGAACCC
    CTGGATATTC CATTGGAGAA GGACAGGACA CCAGAAGCTG TGCCAGAGGA GGTAGTAGAG
    CCAACAGTAC CAGCAGTCCA GTCTCCCCAA GCTGCAGACA GTTACAGAGA AGAAGAACCT
    GGAATGTCCA GGGCCAAATC TAATTGGTTA AGAGCATTCA ACAAGGTGCG CCTGCAGCTG
    CAAGAGGCAC GAGGTGAAGG GACTGGGGAG ATGTCCAAAT CCCTCTGGTT TAAGGATGGG
    CCAGCTGGTG GTCTTATAAT TATAGACAGT ATGCCAGACA TACGAAAGAG GAAACCAATC
    CCGTTGGTCA GTGACCTGGC AATGTCACTG GTTCAGTCAC GGAAGGCTGG GATTACTTCT
    GCTCTTGCAA CAAGCACCTT AAACAATGAA GAGCTAAAAA ACCATGTTTA CAAAAAGACC
    CTGCAAGCTT TAATATACCC AATATCCAGC ACCACTCCCC ACAACTTTGA AGTTTGGACT
    GCCACAACTC CTACCTATTG TTATGAATGC GAAGGGCTTC TGTGGGGAAT CGCTCGGCAG
    GGCATGCGTT GCACAGAGTG TGGTGTCAAG TGCCATGAGA AGTGCCAGGA TCTGCTGAAT
    GCAGATTGTC TTCAAAGAGC TGCAGAGAAG AGCTCAAAAC ATGGTGCAGA AGACCGGACT
    CAGAATATTA TTATGGTCTT GAAGGATCGT ATGAAGATCC GGGAGAGGAA CAAGCCAGAA
    ATATTTGAGC TGATCCAGGA AGTATTTGGG GTATTAAAGG CTACACACGC TCAGCAGATA
    AAGGCTGTGA AACAGAGTGT GCTGGATGGT ACTTCCAAAT GGTCAGCAAA GATCAGCATA
    ACTGTTGTAT GTGCACAGGG TCTACAGGCC AAAGATAAGA CAGGCTCCAG TGACCCATAT
    GTTACTGTTC AAGTTGGGAA AACCAAGAAA AGAACCAAAA CAATCTATGG AAATCTTAAT
    CCTGTCTGGG AAGAGAATTT TCACTTTGAA TGCCACAACT CCTCAGATCG TATTAAAGTA
    CGCGTGTGGG ATGAAGATGA TGATATCAAA TCTCGTGTGA AGCAGAGATT TAAAAGAGAA
    TCAGATGACT TCCTTGGACA AACAATTATT GAAGTCAGAA CACTTAGCGG AGAGATGGAT
    GTGTGGTATA ACTTGGATAA GAGAACAGAT AAATCCGCTG TCTCTGGGGC AATCCGTCTT
    CATATAAGTG TGGAAATAAA AGGAGAGGAA AAGGTGGCTC CCTATCACGT GCAATACACA
    TGTCTACATG AGAACTTATT CCATTACCTG ACGGATGTGC AGAATAATGG TGTGGTCAAG
    ATTCCAGATG CAAAAGGAGA CGACGCCTGG AAAGTTTATT TTGATGAGAC AGCCCAGGAA
    ATTGTGGATG AATTTGCAAT GAGATATGGA GTGGAGTCCA TATACCAGGC CATGACGCAT
    TTTGCCTGCC TTTCATCTAA ATATATGTGT CCAGGAGTAC CAGCTGTAAT GAGTACTCTT
    CTTGCCAACA TTAATGCTTA CTATGCGCAC ACAACTGCAT CTACTAATGT GTCTGCCTCC
    GACCGATTCT CTGCCTCAAA CTTTGGGAAG GAGAGATTTG TAAAGCTGCT TGACCAGTTG
    CACAATTCCT TACGCATTGA CCTGTCCATG TATCGGAACA ACTTCCCTGC TAGCAGCCCT
    GAAAGACTCC AAGATCTCAA GTCCACTGTA GATTTACTAA CTAGCATAAC ATTTTTCCGG
    ATGAAGGTAC AAGAGCTTCA GAGCCCACCA CGGGCAAGCC AAGTTGTTAA GGACTGTGTG
    AAGGCATGCC TTAACTCAAC CTACGAATAT ATCTTTAACA ACTGCCATGA ACTTTATAGC
    CGAGAATATC AAAGTGACCC TGCTAAAAAA GGGGAGGTAC CCCCAGAAGA ACAGGGGCCA
    AGTATAAAGA ACTTAGATTT TTGGTCCAAA CTAATCACAT TGATTGTGTC TATCATAGAG
    GAAGATAAGA ACTCATATAC ACCTTGTCTA AACCAGTTTC CTCAGGAGCT AAATGTTGGT
    AAGATTAGTG CTGAGGTCAT GTGGAGTCTC TTTGCTCAAG ACATGAGATA TGCTATGGAA
    GAACACGACA AACACCGCCT GTGTAAGAGT GCAGACTACA TGAACTTGCA TTTTAAGGTC
    AAGTGGTTGT ACAATGAATA TGTAACTGAG CTACCATCTT TTAAGAGCAA GGTTCCAGAA
    TATCCCGCCT GGTTTGAGCC TTTTGTAATT CAGTGGCTGG ATGAAAATGA AGAGGTCTCC
    CGGGATTTTC TGCATGGAGC ACTTGAAAGA GATAAGAAGG ATGGGTTCCA GCAGACCTCT
    GAACATGCCC TGTTCTCCTG CTCTGTGGTA GATGTTTTCT CACAACTTAA CCAGAGCTTT
    GAGATCATTA AGAAGTTGGA GTGTCCAGAC CCACAAATTG TGGGGCATTA CATGCGTAGA
    TTTGCCAAGA CGATCAGTAA TGTTCTGCTG CAGTATGCTG AAATTATTTC CAAAGATTTT
    GCATCTCATT GCTCCAAAGA GAAGAAAGAA GAAAAAGTGC CCTGTATTCT TATGAACAAT
    ATCCAGCAAC TTCGAGTGCA ACTAGAAAAA ATGTTTGAAG CAATGGGAGG AAAGGAGCTG
    GATGCAGAAA CAAGTGACAT TCTTAAAGAG CTGCAGGTGA AACTCAACAA TGTCCTAGAT
    GAGCTAAGCA GAGTGTTTAC CACCAGTTTT CAGGCACAAA TTGAGGAGTG TGTGAAGCAG
    ATGGGTGACA TTCTGAGCCA GGTAAAAGGG ACCGGAAATG TACCAGCCAG TAACTGCAGC
    AGTGTGGCTC AGGATGCAGA TAATGTATTG CAGCCTATCC TGGATCCTCT GGACAGCAAC
    CTGACACTAT TTGCCAAGAT CTGTGAAAAG ACAGTCCTGA AGCGGGTACT AAAAGAACTC
    TGGAAGCTTG TGATGAATAC AATGGAGAAA ACGATTGTCC TTCCACCACT AACAGACCAA
    ACAATGATAG GGACGCTGCT GAGAAAACAT GGCAAGGGAA TAGAGAAGAG CAGGGTGAAG
    CTGCCGTACC ACGCAGATGG AACCCACCTT ATCATTAATG CAGCCAAGGA ACTGGGCCAG
    TTATCCAAAC TAAAGGATCA CATGGTGCGA GAGGAAGCCA AGAGCCTCAC CCCTAAGCAG
    TGTGCAGTGG TGGAGCTTGC ACTTGATACA ATTAAGCAAT ATTTCCATGC TGGAGGTGTT
    GGTCTCAAGA AGACATTCCT GGAGAAGAGT CCAGACCTAC AATCTCTACG CTATGCACTT
    TCTCTTTACA CACAAGCAAC CGATACGCTA ATCAAAACCT TCGTCCAGAC ACAGTCTGCT
    CAGGGATCTG GGGTGGATGA TTCTGTTGGG GAAGTTTCTA TCCATGTGGA GTTATTTACT
    CACCCAGGAA CAGGCGAGCA CAAGATCAGC GTAAAGGTTG TAGCGGCCAA TGACCTTAAA
    TGGCAGACGT CAGGGATTTT CCGTCCCTTT ATTGAGGTAA ACATGATTGG TCCGAATCTG
    AGTGACAAAA AAAGGAAATT TACCACCAAG TCCAAGAACA ACAGCTGGGC CCCAAAATAT
    AACGAGAGCT TCCAATTTAC CCTGGGTAAT GAGACTGGTC CAGAGAGTTA TGAGCTGCAA
    GTATGTGTGA AAGATTACTG TTTTGCTCGG GAAGACCGCA CAGTGGGAAT GGCTGTCATG
    CAACTGCGAG ATCTGGCACA AAGGGGTAGC TGTGCCTGTT GGCTCCCTCT TGGCCGCCGC
    ATTCATATGG ATGAGACAGG ACTGACCATC TTGCGCATAC TATCACAAAG AAACAATGAT
    GAGGTGGCCA AAGAGTTTGT CAAACTCAAA TCTGACACCC GCTCTCCAGA AGAGGGGAGC
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012810210.1
    CDS188..5230
    Translation

    Target ORF information:

    RefSeq Version XM_012954756.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis unc-13 homolog A (unc13a), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012954756.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCTTTGC TTTGCGTGGG AGTAAAAAAG GCCACGTTTG ATGGAGCACA AGAGAAGTTT 
    AATGCCTACG TAACCTTGAA GGTGCAAAAT GTGAAGAGCA CAACGATAGC GGTGCGGGGA
    AACCAGCCCT GCTGGGAACA GGATTTCATG TTTGAGATTA ATCGTTTAGA CCTTGGTCTA
    ACAGTGGAGG TCTGGAACAA AGGATTGATC TGGGACACCA TGGTGGGAAC AGTATGGATT
    CCTTTAAGGA CAATCCGACA ATCCAATGAG GAGGGTCCTG GGGAATGGCT GACACTTGAT
    TCACAGGTCA TAATGACAGA TAATGAGATT TGTGGTACCA AAGATCCAAC CTTCCACCGC
    ATCTTACTGG ATACCCGATT TGAGCTTCCT CTAGATATAC CAGAAGAAGA AGCTCGATAC
    TGGGCAAAGA AACTCGAGCA ACTTAATGCA ATGAGGGACC AGGATGATTA TGTTTTTCAA
    GAAGAAATTC CGGAGAATCC TCTTCCTGTC CATGGATCTC AATGTTGTAA CTGGAATTAT
    TTCGGCTGGG GGGAGCAGCA CAATGAGGAT CCCGATAGTG CAGTGGATGA TCGGGATAGT
    GATTACCGCA GTGAAACCAG CAACAGTATC CCGCCCCCCT ACTACTCCAC CTCACAGCCT
    AATGCCTCTG TGCACCAGTA CCCTGTACGA CAGCAACACA GTTCTCATGG ATCTTACACT
    GATTCAGTGC ACAGCTATGA GATGGACTTC TCTGACCATC GTCCAATCAG CCCCACTGGA
    AGTAGTCGAT ATGCTTCATC AGGGGAGATT AGTCGTGGCT CCTCACAGCT CAGTGATGAC
    TTTGACCCAG ATGATGACAG CTTGCAAGGT TCAGAAATAG AGGAAGAAAG GGATAGAGAC
    TCTTATCACT CCTGTCATAG TTCTGTCAGC TGCCGCAAGG ACTCGCCAAA TTGGGAAGGA
    GAGGAGGAGC GTGAAGTTTA TAGTGAAGAT GAGGAAGAAT ATCCTGACAG TAGGGGGCAG
    CTCTCTGACC AGGAGCTGTA TGATGATGAC CAGCTTGAGG AAGAAGAGAA AAAAGGAAGA
    TTAGCATATG GGCAGGAGGA TTCCATCTAT CAGTCATTGG AGGCTGAGGA GAAGGAACCC
    CTGGATATTC CATTGGAGAA GGACAGGACA CCAGAAGCTG TGCCAGAGGA GGTAGTAGAG
    CCAACAGTAC CAGCAGTCCA GTCTCCCCAA GCTGCAGACA GTTACAGAGA AGAAGAACCT
    GGAATGTCCA GGGCCAAATC TAATTGGTTA AGAGCATTCA ACAAGGTGCG CCTGCAGCTG
    CAAGAGGCAC GAGGTGAAGG GACTGGGGAG ATGTCCAAAT CCCTCTGGTT TAAGGATGGG
    CCAGCTGGTG GTCTTATAAT TATAGACAGT ATGCCAGACA TACGAAAGAG GAAACCAATC
    CCGTTGGTCA GTGACCTGGC AATGTCACTG GTTCAGTCAC GGAAGGCTGG GATTACTTCT
    GCTCTTGCAA CAAGCACCTT AAACAATGAA GAGCTAAAAA ACCATGTTTA CAAAAAGACC
    CTGCAAGCTT TAATATACCC AATATCCAGC ACCACTCCCC ACAACTTTGA AGTTTGGACT
    GCCACAACTC CTACCTATTG TTATGAATGC GAAGGGCTTC TGTGGGGAAT CGCTCGGCAG
    GGCATGCGTT GCACAGAGTG TGGTGTCAAG TGCCATGAGA AGTGCCAGGA TCTGCTGAAT
    GCAGATTGTC TTCAAAGAGC TGCAGAGAAG AGCTCAAAAC ATGGTGCAGA AGACCGGACT
    CAGAATATTA TTATGGTCTT GAAGGATCGT ATGAAGATCC GGGAGAGGAA CAAGCCAGAA
    ATATTTGAGC TGATCCAGGA AGTATTTGGG GTATTAAAGG CTACACACGC TCAGCAGATA
    AAGGCTGTGA AACAGAGTGT GCTGGATGGT ACTTCCAAAT GGTCAGCAAA GATCAGCATA
    ACTGTTGTAT GTGCACAGGG TCTACAGGCC AAAGATAAGA CAGGCTCCAG TGACCCATAT
    GTTACTGTTC AAGTTGGGAA AACCAAGAAA AGAACCAAAA CAATCTATGG AAATCTTAAT
    CCTGTCTGGG AAGAGAATTT TCACTTTGAA TGCCACAACT CCTCAGATCG TATTAAAGTA
    CGCGTGTGGG ATGAAGATGA TGATATCAAA TCTCGTGTGA AGCAGAGATT TAAAAGAGAA
    TCAGATGACT TCCTTGGACA AACAATTATT GAAGTCAGAA CACTTAGCGG AGAGATGGAT
    GTGTGGTATA ACTTGGATAA GAGAACAGAT AAATCCGCTG TCTCTGGGGC AATCCGTCTT
    CATATAAGTG TGGAAATAAA AGGAGAGGAA AAGGTGGCTC CCTATCACGT GCAATACACA
    TGTCTACATG AGAACTTATT CCATTACCTG ACGGATGTGC AGAATAATGG TGTGGTCAAG
    ATTCCAGATG CAAAAGGAGA CGACGCCTGG AAAGTTTATT TTGATGAGAC AGCCCAGGAA
    ATTGTGGATG AATTTGCAAT GAGATATGGA GTGGAGTCCA TATACCAGGC CATGACGCAT
    TTTGCCTGCC TTTCATCTAA ATATATGTGT CCAGGAGTAC CAGCTGTAAT GAGTACTCTT
    CTTGCCAACA TTAATGCTTA CTATGCGCAC ACAACTGCAT CTACTAATGT GTCTGCCTCC
    GACCGATTCT CTGCCTCAAA CTTTGGGAAG GAGAGATTTG TAAAGCTGCT TGACCAGTTG
    CACAATTCCT TACGCATTGA CCTGTCCATG TATCGGAACA ACTTCCCTGC TAGCAGCCCT
    GAAAGACTCC AAGATCTCAA GTCCACTGTA GATTTACTAA CTAGCATAAC ATTTTTCCGG
    ATGAAGGTAC AAGAGCTTCA GAGCCCACCA CGGGCAAGCC AAGTTGTTAA GGACTGTGTG
    AAGGCATGCC TTAACTCAAC CTACGAATAT ATCTTTAACA ACTGCCATGA ACTTTATAGC
    CGAGAATATC AAAGTGACCC TGCTAAAAAA GGGGAGGTAC CCCCAGAAGA ACAGGGGCCA
    AGTATAAAGA ACTTAGATTT TTGGTCCAAA CTAATCACAT TGATTGTGTC TATCATAGAG
    GAAGATAAGA ACTCATATAC ACCTTGTCTA AACCAGTTTC CTCAGGAGCT AAATGTTGGT
    AAGATTAGTG CTGAGGTCAT GTGGAGTCTC TTTGCTCAAG ACATGAGATA TGCTATGGAA
    GAACACGACA AACACCGCCT GTGTAAGAGT GCAGACTACA TGAACTTGCA TTTTAAGGTC
    AAGTGGTTGT ACAATGAATA TGTAACTGAG CTACCATCTT TTAAGAGCAA GGTTCCAGAA
    TATCCCGCCT GGTTTGAGCC TTTTGTAATT CAGTGGCTGG ATGAAAATGA AGAGGTCTCC
    CGGGATTTTC TGCATGGAGC ACTTGAAAGA GATAAGAAGG ATGGGTTCCA GCAGACCTCT
    GAACATGCCC TGTTCTCCTG CTCTGTGGTA GATGTTTTCT CACAACTTAA CCAGAGCTTT
    GAGATCATTA AGAAGTTGGA GTGTCCAGAC CCACAAATTG TGGGGCATTA CATGCGTAGA
    TTTGCCAAGA CGATCAGTAA TGTTCTGCTG CAGTATGCTG AAATTATTTC CAAAGATTTT
    GCATCTCATT GCTCCAAAGA GAAGAAAGAA GAAAAAGTGC CCTGTATTCT TATGAACAAT
    ATCCAGCAAC TTCGAGTGCA ACTAGAAAAA ATGTTTGAAG CAATGGGAGG AAAGGAGCTG
    GATGCAGAAA CAAGTGACAT TCTTAAAGAG CTGCAGGTGA AACTCAACAA TGTCCTAGAT
    GAGCTAAGCA GAGTGTTTAC CACCAGTTTT CAGGCACAAA TTGAGGAGTG TGTGAAGCAG
    ATGGGTGACA TTCTGAGCCA GGTAAAAGGG ACCGGAAATG TACCAGCCAG TAACTGCAGC
    AGTGTGGCTC AGGATGCAGA TAATGTATTG CAGCCTATCC TGGATCCTCT GGACAGCAAC
    CTGACACTAT TTGCCAAGAT CTGTGAAAAG ACAGTCCTGA AGCGGGTACT AAAAGAACTC
    TGGAAGCTTG TGATGAATAC AATGGAGAAA ACGATTGTCC TTCCACCACT AACAGACCAA
    ACAATGATAG GGACGCTGCT GAGAAAACAT GGCAAGGGAA TAGAGAAGAG CAGGGTGAAG
    CTGCCGTACC ACGCAGATGG AACCCACCTT ATCATTAATG CAGCCAAGGA ACTGGGCCAG
    TTATCCAAAC TAAAGGATCA CATGGTGCGA GAGGAAGCCA AGAGCCTCAC CCCTAAGCAG
    TGTGCAGTGG TGGAGCTTGC ACTTGATACA ATTAAGCAAT ATTTCCATGC TGGAGGTGTT
    GGTCTCAAGA AGACATTCCT GGAGAAGAGT CCAGACCTAC AATCTCTACG CTATGCACTT
    TCTCTTTACA CACAAGCAAC CGATACGCTA ATCAAAACCT TCGTCCAGAC ACAGTCTGCT
    CAGGGATCTG GGGTGGATGA TTCTGTTGGG GAAGTTTCTA TCCATGTGGA GTTATTTACT
    CACCCAGGAA CAGGCGAGCA CAAGATCAGC GTAAAGGTTG TAGCGGCCAA TGACCTTAAA
    TGGCAGACGT CAGGGATTTT CCGTCCCTTT ATTGAGGTAA ACATGATTGG TCCGAATCTG
    AGTGACAAAA AAAGGAAATT TACCACCAAG TCCAAGAACA ACAGCTGGGC CCCAAAATAT
    AACGAGAGCT TCCAATTTAC CCTGGGTAAT GAGACTGGTC CAGAGAGTTA TGAGCTGCAA
    GTATGTGTGA AAGATTACTG TTTTGCTCGG GAAGACCGCA CAGTGGGAAT GGCTGTCATG
    CAACTGCGAG ATCTGGCACA AAGGGGTAGC TGTGCCTGTT GGCTCCCTCT TGGCCGCCGC
    ATTCATATGG ATGAGACAGG ACTGACCATC TTGCGCATAC TATCACAAAG AAACAATGAT
    GAGGTGGCCA AAGAGTTTGT CAAACTCAAA TCTGACACCC GCTCTCCAGA AGAGGGGAGC
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.