nedd4l gene cDNA ORF clone, Danio rerio(zebrafish)

mRNA	Protein	Name
XM_021469019.1	XP_021324694.1	E3 ubiquitin-protein ligase NEDD4-like isoform X8
XM_021469020.1	XP_021324695.1	E3 ubiquitin-protein ligase NEDD4-like isoform X9
XM_021469023.1	XP_021324698.1	E3 ubiquitin-protein ligase NEDD4-like isoform X12
XM_021469025.1	XP_021324700.1	E3 ubiquitin-protein ligase NEDD4-like isoform X14
XM_005161087.2	XP_005161144.1	E3 ubiquitin-protein ligase NEDD4-like isoform X16
XM_009295228.2	XP_009293503.1	E3 ubiquitin-protein ligase NEDD4-like isoform X3
XM_009295231.2	XP_009293506.1	E3 ubiquitin-protein ligase NEDD4-like isoform X6
XM_005161088.2	XP_005161145.1	E3 ubiquitin-protein ligase NEDD4-like isoform X9
XM_005161083.3	XP_005161140.1	E3 ubiquitin-protein ligase NEDD4-like isoform X1
XM_009295229.2	XP_009293504.1	E3 ubiquitin-protein ligase NEDD4-like isoform X4
XM_021469014.1	XP_021324689.1	E3 ubiquitin-protein ligase NEDD4-like isoform X3
XM_021469024.1	XP_021324699.1	E3 ubiquitin-protein ligase NEDD4-like isoform X13
XM_009295230.2	XP_009293505.1	E3 ubiquitin-protein ligase NEDD4-like isoform X5
XM_005161085.2	XP_005161142.1	E3 ubiquitin-protein ligase NEDD4-like isoform X7
XM_021469013.1	XP_021324688.1	E3 ubiquitin-protein ligase NEDD4-like isoform X2
XM_021469016.1	XP_021324691.1	E3 ubiquitin-protein ligase NEDD4-like isoform X5
XM_021469017.1	XP_021324692.1	E3 ubiquitin-protein ligase NEDD4-like isoform X6
XM_021469022.1	XP_021324697.1	E3 ubiquitin-protein ligase NEDD4-like isoform X11
XM_009295227.2	XP_009293502.1	E3 ubiquitin-protein ligase NEDD4-like isoform X2
XM_021469021.1	XP_021324696.1	E3 ubiquitin-protein ligase NEDD4-like isoform X10
XM_682997.7	XP_688089.3	E3 ubiquitin-protein ligase NEDD4-like isoform X15
XM_021469012.1	XP_021324687.1	E3 ubiquitin-protein ligase NEDD4-like isoform X1
XM_021469015.1	XP_021324690.1	E3 ubiquitin-protein ligase NEDD4-like isoform X4
XM_021469018.1	XP_021324693.1	E3 ubiquitin-protein ligase NEDD4-like isoform X7

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa25659	XM_005161087.2 Latest version!	Danio rerio neural precursor cell expressed, developmentally down-regulated 4-like (nedd4l), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa25660	XM_005161088.2 Latest version!	Danio rerio neural precursor cell expressed, developmentally down-regulated 4-like (nedd4l), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa25661	XM_682997.7 Latest version!	Danio rerio neural precursor cell expressed, developmentally down-regulated 4-like (nedd4l), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa151011	XM_021469012.1 Latest version!	Danio rerio neural precursor cell expressed, developmentally down-regulated 4-like (nedd4l), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
ODa151012	XM_021469013.1 Latest version!	Danio rerio neural precursor cell expressed, developmentally down-regulated 4-like (nedd4l), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
ODa151013	XM_021469014.1 Latest version!	Danio rerio neural precursor cell expressed, developmentally down-regulated 4-like (nedd4l), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
ODa151014	XM_021469015.1 Latest version!	Danio rerio neural precursor cell expressed, developmentally down-regulated 4-like (nedd4l), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
ODa151015	XM_021469016.1 Latest version!	Danio rerio neural precursor cell expressed, developmentally down-regulated 4-like (nedd4l), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
ODa151016	XM_021469017.1 Latest version!	Danio rerio neural precursor cell expressed, developmentally down-regulated 4-like (nedd4l), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
ODa151017	XM_021469018.1 Latest version!	Danio rerio neural precursor cell expressed, developmentally down-regulated 4-like (nedd4l), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
ODa151018	XM_021469019.1 Latest version!	Danio rerio neural precursor cell expressed, developmentally down-regulated 4-like (nedd4l), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
ODa151019	XM_021469020.1 Latest version!	Danio rerio neural precursor cell expressed, developmentally down-regulated 4-like (nedd4l), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
ODa151020	XM_021469021.1 Latest version!	Danio rerio neural precursor cell expressed, developmentally down-regulated 4-like (nedd4l), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
ODa151021	XM_021469022.1 Latest version!	Danio rerio neural precursor cell expressed, developmentally down-regulated 4-like (nedd4l), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
ODa151022	XM_021469023.1 Latest version!	Danio rerio neural precursor cell expressed, developmentally down-regulated 4-like (nedd4l), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
ODa151023	XM_021469024.1 Latest version!	Danio rerio neural precursor cell expressed, developmentally down-regulated 4-like (nedd4l), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
ODa151024	XM_021469025.1 Latest version!	Danio rerio neural precursor cell expressed, developmentally down-regulated 4-like (nedd4l), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
ODa25656	XM_005161083.3 Latest version!	Danio rerio neural precursor cell expressed, developmentally down-regulated 4-like (nedd4l), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa25658	XM_005161085.2 Latest version!	Danio rerio neural precursor cell expressed, developmentally down-regulated 4-like (nedd4l), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa43142	XM_009295227.2 Latest version!	Danio rerio neural precursor cell expressed, developmentally down-regulated 4-like (nedd4l), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa43143	XM_009295228.2 Latest version!	Danio rerio neural precursor cell expressed, developmentally down-regulated 4-like (nedd4l), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa43144	XM_009295229.2 Latest version!	Danio rerio neural precursor cell expressed, developmentally down-regulated 4-like (nedd4l), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa43145	XM_009295230.2 Latest version!	Danio rerio neural precursor cell expressed, developmentally down-regulated 4-like (nedd4l), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa43146	XM_009295231.2 Latest version!	Danio rerio neural precursor cell expressed, developmentally down-regulated 4-like (nedd4l), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

nedd4l ( XM_005161087.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005161144.1 Danio rerio neural precursor cell expressed, developmentally down-regulated 4-like (nedd4l), transcript variant X16, mRNA.

Price & Availability↑

CloneID	ODa25659
Clone ID Related Accession (Same CDS sequence)	XM_005161087.2
Accession Version	XM_005161087.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2991bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase NEDD4-like isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 24, 2014 this sequence version replaced XM_005161087.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTACGA ATTTTGAACC CATTTATGGA CTTTCAGAGG ATGAAAACGA ATCTCGTATT 
CTTCGCGTCA AGGTCATTGC TGGCATTGAT CTTGCGAAGA AGGACATCAT TGGTGCCAGT 
GATCCATACG TCAAACTCTC CCTGTATGTC GCTGATGAAA ACCGCGAGCT TGCCTTAGTA 
CAAACGAAAA CCATCAAAAA GACTCTTAAT CCCAAATGGA ACGAGGAATT CTATTTCAGA 
GTCTGCCCAC AAAACCACAG GCTTCTTTTC GAGGTGTTTG ACGAAAACCG CTTGACCAGA 
GATGACTTCC TGGGGCAGGT GGACGTGCCT CTTACACACT TACCGACTGA AGACCCTGCC 
ATGGAGCGTC CATACACGTT TAAAGACTTT CTCCTGCGTC CAAGAAGTCA TAAATCTCGG 
GTGAAAGGTT ACCTGAGGCT CAAGATGGCC TACCTGCCAA AGAATGGAGG ACATGAGGAG 
GAAAGCGGTG AAATGAGAGA GGAGGCTGAG GGTTGGGAAG TGACAGACTC CACAGATCCA 
GGATCTCAGA GACCTCAGCA GCTCCTGCCT CCTCTTCCTC CGGGCTGGGA AGAGAAAGTG 
GATAACCTGG GACGGACGTA CTATGTGAAC CACAACAACC GCACCACACA GTGGAAGAGG 
CCCAGCAGTG TGGATGTGGT TTCAGAAACG GAAAATGATA ATCAATTGCG GCAGATTAAC 
CAGGAAGCCC ACAGAGTTTT CCGCTCTCGA CGGCACATTA GCGAAGACCT GGAAAATGAG 
CAGCTGGACA TCAGGGACAT AGACGACTCA TGGGAGCCCA TATCAGAGGA AGATCCGACT 
TTATTAACAG ATGGTCTGAA CCATTCTCTG TCGGGACCCT CCTCTCTGGC AATGCCCCTC 
TCCAGCACTC CAGACTTCTC AGATGAGATC AGCCTTAGAT TGTCTCTCGC TCCAGACACC 
AACGGAGAAA TCCCGGGACC CAGCTCTGCT GTGCAGAGTC ATCTTTCCTC TCGGCTACGC 
TCCTCTAGTA TGACTGATGG AGTCAGTGAT CAAGCTCAAC CTCCCACACC AACGCAGAGC 
TCCCAGACCC GACGAACAAG AGCACAAACC GTCACAGGCG TTGAGGACAC CATGTCTCCT 
TCGGCCTCTT ACGCCTTGAC CACGCCAGGC CTTCCACCAG GCTGGGAGGA ACGCAAAGAC 
CCCAAGGGAC GAACGTACTA CGTTAATCAT AACAACCGTA GCACCACATG GACAAGGCCC 
ATTCTGCAGC AGCACACTGA GGATGGAGCC AGCACCTCGG TTGCAGCAGT AGGGGGCGCT 
ACAGCAGCTA CACCCCCTGC TTCAACCCCT TCCACCTCCA GCGGCCATCT AAGCGAACCA 
CAAGTGCGCA GACCCCGTAG CCTCAGTTCC CCTACCGTCA CTCTCTCTTC CCCACTAGAG 
GGAGCCAATA ACGTCCCTAT ACGGCGAGCC GTAAAGGACA CATTCTCCAA CCCTCAGTCT 
CCACAGCCTT CGCCCTACAG CTCACCTAAA ACCCAGCATA AAGCCAACCA GAGCTTCCTG 
CCACCGGGAT GGGAAATGAG GATCGCACCC AACGGACGGC CGTTCTTCAT CGACCACAAC 
AGCAGAACCA CCACATGGGA GGATCCCCGC TTGAAGTATC CCGTTCACAT GAGGACAAAA 
GCGTCTCTAG ACCCGGGCGA CCTCGGCCCA CTTCCTCCTG GGTGGGAGGA ACGAGTGCAT 
GCTGATGGAA GAACCTTTTA CATCGATCAC AACAACAAAA AAACACAGTG GGAAGACCCT 
CGTCTCCAGA GTCCTGCCAT AACTGGACCC GCGGTGCCGT ATTCAAGAGA ATTCAAGCAG 
AAATACGACT ACTTCAGGAA GAAGTTAAAG AAACCGGCAG ATATCCCCAA CCGCTTTGAG 
ATGAAGCTGC ACAGGAATAA CATCTTTGAG GAGTCCTACA GACGAATCAT GTCCCTCAAG 
AGACCTGACA GCCTGAAGGC CCGTCTGTGG ATTGAGTTTG AGTCAGAGAA GGGTCTGGAT 
TATGGCGGTG TGGCCCGAGA ATGGTTCTTC CTGCTCTCTA AGGAGATGTT CAACCCTTAT 
TATGGGCTGT TCGAGTACTC GGCCACAGAT AACTACACCC TTCAGATCAA TCCCAACTCT 
GGCCTGTGTA ATGAGGATCA CCTCTCATAC TTCAAGTTCA TTGGTCGAGT GGCCGGCATG 
GCTGTTTATC ATGGCAAACT CTTGGACGGT TTCTTCATTC GACCGTTTTA TAAGATGATG 
CTGGGCAAAC AGATCACACT GAATGACATG GAGTCAGTGG ACAGCGAATA CTACAACTCC 
CTGAAGTGGA TTCTGGAAAA CGATCCCACA GAATTAGACT TACGGTTTTG TATTGATGAG 
GACAACTTTG GACAGACATA TCAGGTAGAT TTGAAGCCAA GCGGATCAGA CATGGTTGTA 
ACCAACGACA ACAAGAAGGA ATACATCGAT CTGGTGATTC AGTGGAGGTT TGTGAACAGG 
GTCCAGAAAC AGATGAACGC CTTTTTGGAA GGCTTCACTG AACTCATTCC CATCGACTTA 
ATAAAGATTT TTGATGAAAA CGAACTGGAG CTGTTGATGT GTGGTCTGGG AGATGTGGAT 
GTCAATGACT GGAGGCAACA CACTGTCTAT AAGAACGGCT ACTGTCCAAA TCACCCCGTC 
ATCCAGTGGT TCTGGAAGGC TGTGTTGTTA ATGGATGCAG AAAAGCGCAT CAGGCTTCTA 
CAGTTTGTCA CTGGAACATC CAGAGTGCCA ATGAACGGCT TTGCTGAACT CTACGGTTCC 
AATGGGCCGC AGTTGTTTAC TATTGAGCAG TGGGGAACTC CTGACAAACT CCCTCGGGCT 
CACACCTGCT TTAATCGCTT GGACCTCCCA ATGTATGAAA CGTTTGAAGA CCTGCGAGAG 
AAGCTTCTGA TGGCGGTGGA AAACGCTCAA GGCTTTGAGG GCGTCGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005161144.1
CDS	304..3294
Translation	MATNFEPIYGLSEDENESRILRVKVIAGIDLAKKDIIGASDPYVKLSLYVADENRELALVQTKTIKKTLNPKWNEEFYFRVCPQNHRLLFEVFDENRLTRDDFLGQVDVPLTHLPTEDPAMERPYTFKDFLLRPRSHKSRVKGYLRLKMAYLPKNGGHEEESGEMREEAEGWEVTDSTDPGSQRPQQLLPPLPPGWEEKVDNLGRTYYVNHNNRTTQWKRPSSVDVVSETENDNQLRQINQEAHRVFRSRRHISEDLENEQLDIRDIDDSWEPISEEDPTLLTDGLNHSLSGPSSLAMPLSSTPDFSDEISLRLSLAPDTNGEIPGPSSAVQSHLSSRLRSSSMTDGVSDQAQPPTPTQSSQTRRTRAQTVTGVEDTMSPSASYALTTPGLPPGWEERKDPKGRTYYVNHNNRSTTWTRPILQQHTEDGASTSVAAVGGATAATPPASTPSTSSGHLSEPQVRRPRSLSSPTVTLSSPLEGANNVPIRRAVKDTFSNPQSPQPSPYSSPKTQHKANQSFLPPGWEMRIAPNGRPFFIDHNSRTTTWEDPRLKYPVHMRTKASLDPGDLGPLPPGWEERVHADGRTFYIDHNNKKTQWEDPRLQSPAITGPAVPYSREFKQKYDYFRKKLKKPADIPNRFEMKLHRNNIFEESYRRIMSLKRPDSLKARLWIEFESEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFKFIGRVAGMAVYHGKLLDGFFIRPFYKMMLGKQITLNDMESVDSEYYNSLKWILENDPTELDLRFCIDEDNFGQTYQVDLKPSGSDMVVTNDNKKEYIDLVIQWRFVNRVQKQMNAFLEGFTELIPIDLIKIFDENELELLMCGLGDVDVNDWRQHTVYKNGYCPNHPVIQWFWKAVLLMDAEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGTPDKLPRAHTCFNRLDLPMYETFEDLREKLLMAVENAQGFEGVD

Target ORF information:

RefSeq Version	XM_005161087.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio neural precursor cell expressed, developmentally down-regulated 4-like (nedd4l), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005161087.2

ORF Insert Sequence:

ATGGCTACGA ATTTTGAACC CATTTATGGA CTTTCAGAGG ATGAAAACGA ATCTCGTATT 
CTTCGCGTCA AGGTCATTGC TGGCATTGAT CTTGCGAAGA AGGACATCAT TGGTGCCAGT 
GATCCATACG TCAAACTCTC CCTGTATGTC GCTGATGAAA ACCGCGAGCT TGCCTTAGTA 
CAAACGAAAA CCATCAAAAA GACTCTTAAT CCCAAATGGA ACGAGGAATT CTATTTCAGA 
GTCTGCCCAC AAAACCACAG GCTTCTTTTC GAGGTGTTTG ACGAAAACCG CTTGACCAGA 
GATGACTTCC TGGGGCAGGT GGACGTGCCT CTTACACACT TACCGACTGA AGACCCTGCC 
ATGGAGCGTC CATACACGTT TAAAGACTTT CTCCTGCGTC CAAGAAGTCA TAAATCTCGG 
GTGAAAGGTT ACCTGAGGCT CAAGATGGCC TACCTGCCAA AGAATGGAGG ACATGAGGAG 
GAAAGCGGTG AAATGAGAGA GGAGGCTGAG GGTTGGGAAG TGACAGACTC CACAGATCCA 
GGATCTCAGA GACCTCAGCA GCTCCTGCCT CCTCTTCCTC CGGGCTGGGA AGAGAAAGTG 
GATAACCTGG GACGGACGTA CTATGTGAAC CACAACAACC GCACCACACA GTGGAAGAGG 
CCCAGCAGTG TGGATGTGGT TTCAGAAACG GAAAATGATA ATCAATTGCG GCAGATTAAC 
CAGGAAGCCC ACAGAGTTTT CCGCTCTCGA CGGCACATTA GCGAAGACCT GGAAAATGAG 
CAGCTGGACA TCAGGGACAT AGACGACTCA TGGGAGCCCA TATCAGAGGA AGATCCGACT 
TTATTAACAG ATGGTCTGAA CCATTCTCTG TCGGGACCCT CCTCTCTGGC AATGCCCCTC 
TCCAGCACTC CAGACTTCTC AGATGAGATC AGCCTTAGAT TGTCTCTCGC TCCAGACACC 
AACGGAGAAA TCCCGGGACC CAGCTCTGCT GTGCAGAGTC ATCTTTCCTC TCGGCTACGC 
TCCTCTAGTA TGACTGATGG AGTCAGTGAT CAAGCTCAAC CTCCCACACC AACGCAGAGC 
TCCCAGACCC GACGAACAAG AGCACAAACC GTCACAGGCG TTGAGGACAC CATGTCTCCT 
TCGGCCTCTT ACGCCTTGAC CACGCCAGGC CTTCCACCAG GCTGGGAGGA ACGCAAAGAC 
CCCAAGGGAC GAACGTACTA CGTTAATCAT AACAACCGTA GCACCACATG GACAAGGCCC 
ATTCTGCAGC AGCACACTGA GGATGGAGCC AGCACCTCGG TTGCAGCAGT AGGGGGCGCT 
ACAGCAGCTA CACCCCCTGC TTCAACCCCT TCCACCTCCA GCGGCCATCT AAGCGAACCA 
CAAGTGCGCA GACCCCGTAG CCTCAGTTCC CCTACCGTCA CTCTCTCTTC CCCACTAGAG 
GGAGCCAATA ACGTCCCTAT ACGGCGAGCC GTAAAGGACA CATTCTCCAA CCCTCAGTCT 
CCACAGCCTT CGCCCTACAG CTCACCTAAA ACCCAGCATA AAGCCAACCA GAGCTTCCTG 
CCACCGGGAT GGGAAATGAG GATCGCACCC AACGGACGGC CGTTCTTCAT CGACCACAAC 
AGCAGAACCA CCACATGGGA GGATCCCCGC TTGAAGTATC CCGTTCACAT GAGGACAAAA 
GCGTCTCTAG ACCCGGGCGA CCTCGGCCCA CTTCCTCCTG GGTGGGAGGA ACGAGTGCAT 
GCTGATGGAA GAACCTTTTA CATCGATCAC AACAACAAAA AAACACAGTG GGAAGACCCT 
CGTCTCCAGA GTCCTGCCAT AACTGGACCC GCGGTGCCGT ATTCAAGAGA ATTCAAGCAG 
AAATACGACT ACTTCAGGAA GAAGTTAAAG AAACCGGCAG ATATCCCCAA CCGCTTTGAG 
ATGAAGCTGC ACAGGAATAA CATCTTTGAG GAGTCCTACA GACGAATCAT GTCCCTCAAG 
AGACCTGACA GCCTGAAGGC CCGTCTGTGG ATTGAGTTTG AGTCAGAGAA GGGTCTGGAT 
TATGGCGGTG TGGCCCGAGA ATGGTTCTTC CTGCTCTCTA AGGAGATGTT CAACCCTTAT 
TATGGGCTGT TCGAGTACTC GGCCACAGAT AACTACACCC TTCAGATCAA TCCCAACTCT 
GGCCTGTGTA ATGAGGATCA CCTCTCATAC TTCAAGTTCA TTGGTCGAGT GGCCGGCATG 
GCTGTTTATC ATGGCAAACT CTTGGACGGT TTCTTCATTC GACCGTTTTA TAAGATGATG 
CTGGGCAAAC AGATCACACT GAATGACATG GAGTCAGTGG ACAGCGAATA CTACAACTCC 
CTGAAGTGGA TTCTGGAAAA CGATCCCACA GAATTAGACT TACGGTTTTG TATTGATGAG 
GACAACTTTG GACAGACATA TCAGGTAGAT TTGAAGCCAA GCGGATCAGA CATGGTTGTA 
ACCAACGACA ACAAGAAGGA ATACATCGAT CTGGTGATTC AGTGGAGGTT TGTGAACAGG 
GTCCAGAAAC AGATGAACGC CTTTTTGGAA GGCTTCACTG AACTCATTCC CATCGACTTA 
ATAAAGATTT TTGATGAAAA CGAACTGGAG CTGTTGATGT GTGGTCTGGG AGATGTGGAT 
GTCAATGACT GGAGGCAACA CACTGTCTAT AAGAACGGCT ACTGTCCAAA TCACCCCGTC 
ATCCAGTGGT TCTGGAAGGC TGTGTTGTTA ATGGATGCAG AAAAGCGCAT CAGGCTTCTA 
CAGTTTGTCA CTGGAACATC CAGAGTGCCA ATGAACGGCT TTGCTGAACT CTACGGTTCC 
AATGGGCCGC AGTTGTTTAC TATTGAGCAG TGGGGAACTC CTGACAAACT CCCTCGGGCT 
CACACCTGCT TTAATCGCTT GGACCTCCCA ATGTATGAAA CGTTTGAAGA CCTGCGAGAG 
AAGCTTCTGA TGGCGGTGGA AAACGCTCAA GGCTTTGAGG GCGTCGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nedd4l ( XM_005161088.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005161145.1 Danio rerio neural precursor cell expressed, developmentally down-regulated 4-like (nedd4l), transcript variant X9, mRNA.

Price & Availability↑

CloneID	ODa25660
Clone ID Related Accession (Same CDS sequence)	XM_005161088.2
Accession Version	XM_005161088.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2988bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	E3 ubiquitin-protein ligase NEDD4-like isoform X9
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 24, 2014 this sequence version replaced XM_005161088.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTACGA ATTTTGAACC CATTTATGGA CTTTCAGAGG ATGAAAACGA ATCTCGTATT 
CTTCGCGTCA AGGTCATTGC TGGCATTGAT CTTGCGAAGA AGGACATCAT TGGTGCCAGT 
GATCCATACG TCAAACTCTC CCTGTATGTC GCTGATGAAA ACCGCGAGCT TGCCTTAGTA 
CAAACGAAAA CCATCAAAAA GACTCTTAAT CCCAAATGGA ACGAGGAATT CTATTTCAGA 
GTCTGCCCAC AAAACCACAG GCTTCTTTTC GAGGTGTTTG ACGAAAACCG CTTGACCAGA 
GATGACTTCC TGGGGCAGGT GGACGTGCCT CTTACACACT TACCGACTGA AGACCCTGCC 
ATGGAGCGTC CATACACGTT TAAAGACTTT CTCCTGCGTC CAAGAAGTCA TAAATCTCGG 
GTGAAAGGTT ACCTGAGGCT CAAGATGGCC TACCTGCCAA AGAATGGAGG ACATGAGGAG 
GAAAGCGGTG AAATGAGAGA GGAGGCTGAG GGTTGGGAAG TGACAGACTC CACAGATCCA 
GGATCTCAGA GACCTCAGCA GCTCCTGCCT CCTCTTCCTC CGGGCTGGGA AGAGAAAGTG 
GATAACCTGG GACGGACGTA CTATGTGAAC CACAACAACC GCACCACACA GTGGAAGAGG 
CCCAGCAGTG TGGATGTGGT TTCAGAAACG GAAAATGATA ATCAATTGCG GCAGATTAAC 
CAGGAAGCCC ACAGAGTTTT CCGCTCTCGA CGGCACATTA GCGAAGACCT GGAAAATGAG 
CAGCTGGACA TCAGGGACAT AGACGACTCA TGGGAGCCCA TATCAGAGGA AGATCCGACT 
TTATTAACAG ATGGTCTGAA CCATTCTCTG TCGGGACCCT CCTCTCTGGC AATGCCCCTC 
TCCAGCACTC CAGACTTCTC AGATGAGATC AGCCTTAGAT TGTCTCTCGC TCCAGACACC 
AACGGAGAAA TCCCGGGACC CAGCTCTGCT GTGCAGAGTC ATCTTTCCTC TCGGCTACGC 
TCCTCTAGTA TGACTGATGG AGTCAGTGAT CAAGCTCAAC CTCCCACACC AACGAGCTCC 
CAGACCCGAC GAACAAGAGC ACAAACCGTC ACAGGCGTTG AGGACACCAT GTCTCCTTCG 
GCCTCTTACG CCTTGACCAC GCCAGGCCTT CCACCAGGCT GGGAGGAACG CAAAGACCCC 
AAGGGACGAA CGTACTACGT TAATCATAAC AACCGTAGCA CCACATGGAC AAGGCCCATT 
CTGCAGCAGC ACACTGAGGA TGGAGCCAGC ACCTCGGTTG CAGCAGTAGG GGGCGCTACA 
GCAGCTACAC CCCCTGCTTC AACCCCTTCC ACCTCCAGCG GCCATCTAAG CGAACCACAA 
GTGCGCAGAC CCCGTAGCCT CAGTTCCCCT ACCGTCACTC TCTCTTCCCC ACTAGAGGGA 
GCCAATAACG TCCCTATACG GCGAGCCGTA AAGGACACAT TCTCCAACCC TCAGTCTCCA 
CAGCCTTCGC CCTACAGCTC ACCTAAAACC CAGCATAAAG CCAACCAGAG CTTCCTGCCA 
CCGGGATGGG AAATGAGGAT CGCACCCAAC GGACGGCCGT TCTTCATCGA CCACAACAGC 
AGAACCACCA CATGGGAGGA TCCCCGCTTG AAGTATCCCG TTCACATGAG GACAAAAGCG 
TCTCTAGACC CGGGCGACCT CGGCCCACTT CCTCCTGGGT GGGAGGAACG AGTGCATGCT 
GATGGAAGAA CCTTTTACAT CGATCACAAC AACAAAAAAA CACAGTGGGA AGACCCTCGT 
CTCCAGAGTC CTGCCATAAC TGGACCCGCG GTGCCGTATT CAAGAGAATT CAAGCAGAAA 
TACGACTACT TCAGGAAGAA GTTAAAGAAA CCGGCAGATA TCCCCAACCG CTTTGAGATG 
AAGCTGCACA GGAATAACAT CTTTGAGGAG TCCTACAGAC GAATCATGTC CCTCAAGAGA 
CCTGACAGCC TGAAGGCCCG TCTGTGGATT GAGTTTGAGT CAGAGAAGGG TCTGGATTAT 
GGCGGTGTGG CCCGAGAATG GTTCTTCCTG CTCTCTAAGG AGATGTTCAA CCCTTATTAT 
GGGCTGTTCG AGTACTCGGC CACAGATAAC TACACCCTTC AGATCAATCC CAACTCTGGC 
CTGTGTAATG AGGATCACCT CTCATACTTC AAGTTCATTG GTCGAGTGGC CGGCATGGCT 
GTTTATCATG GCAAACTCTT GGACGGTTTC TTCATTCGAC CGTTTTATAA GATGATGCTG 
GGCAAACAGA TCACACTGAA TGACATGGAG TCAGTGGACA GCGAATACTA CAACTCCCTG 
AAGTGGATTC TGGAAAACGA TCCCACAGAA TTAGACTTAC GGTTTTGTAT TGATGAGGAC 
AACTTTGGAC AGACATATCA GGTAGATTTG AAGCCAAGCG GATCAGACAT GGTTGTAACC 
AACGACAACA AGAAGGAATA CATCGATCTG GTGATTCAGT GGAGGTTTGT GAACAGGGTC 
CAGAAACAGA TGAACGCCTT TTTGGAAGGC TTCACTGAAC TCATTCCCAT CGACTTAATA 
AAGATTTTTG ATGAAAACGA ACTGGAGCTG TTGATGTGTG GTCTGGGAGA TGTGGATGTC 
AATGACTGGA GGCAACACAC TGTCTATAAG AACGGCTACT GTCCAAATCA CCCCGTCATC 
CAGTGGTTCT GGAAGGCTGT GTTGTTAATG GATGCAGAAA AGCGCATCAG GCTTCTACAG 
TTTGTCACTG GAACATCCAG AGTGCCAATG AACGGCTTTG CTGAACTCTA CGGTTCCAAT 
GGGCCGCAGT TGTTTACTAT TGAGCAGTGG GGAACTCCTG ACAAACTCCC TCGGGCTCAC 
ACCTGCTTTA ATCGCTTGGA CCTCCCAATG TATGAAACGT TTGAAGACCT GCGAGAGAAG 
CTTCTGATGG CGGTGGAAAA CGCTCAAGGC TTTGAGGGCG TCGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005161145.1
CDS	304..3291
Translation	MATNFEPIYGLSEDENESRILRVKVIAGIDLAKKDIIGASDPYVKLSLYVADENRELALVQTKTIKKTLNPKWNEEFYFRVCPQNHRLLFEVFDENRLTRDDFLGQVDVPLTHLPTEDPAMERPYTFKDFLLRPRSHKSRVKGYLRLKMAYLPKNGGHEEESGEMREEAEGWEVTDSTDPGSQRPQQLLPPLPPGWEEKVDNLGRTYYVNHNNRTTQWKRPSSVDVVSETENDNQLRQINQEAHRVFRSRRHISEDLENEQLDIRDIDDSWEPISEEDPTLLTDGLNHSLSGPSSLAMPLSSTPDFSDEISLRLSLAPDTNGEIPGPSSAVQSHLSSRLRSSSMTDGVSDQAQPPTPTSSQTRRTRAQTVTGVEDTMSPSASYALTTPGLPPGWEERKDPKGRTYYVNHNNRSTTWTRPILQQHTEDGASTSVAAVGGATAATPPASTPSTSSGHLSEPQVRRPRSLSSPTVTLSSPLEGANNVPIRRAVKDTFSNPQSPQPSPYSSPKTQHKANQSFLPPGWEMRIAPNGRPFFIDHNSRTTTWEDPRLKYPVHMRTKASLDPGDLGPLPPGWEERVHADGRTFYIDHNNKKTQWEDPRLQSPAITGPAVPYSREFKQKYDYFRKKLKKPADIPNRFEMKLHRNNIFEESYRRIMSLKRPDSLKARLWIEFESEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFKFIGRVAGMAVYHGKLLDGFFIRPFYKMMLGKQITLNDMESVDSEYYNSLKWILENDPTELDLRFCIDEDNFGQTYQVDLKPSGSDMVVTNDNKKEYIDLVIQWRFVNRVQKQMNAFLEGFTELIPIDLIKIFDENELELLMCGLGDVDVNDWRQHTVYKNGYCPNHPVIQWFWKAVLLMDAEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGTPDKLPRAHTCFNRLDLPMYETFEDLREKLLMAVENAQGFEGVD

Target ORF information:

RefSeq Version	XM_005161088.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio neural precursor cell expressed, developmentally down-regulated 4-like (nedd4l), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005161088.2

ORF Insert Sequence:

ATGGCTACGA ATTTTGAACC CATTTATGGA CTTTCAGAGG ATGAAAACGA ATCTCGTATT 
CTTCGCGTCA AGGTCATTGC TGGCATTGAT CTTGCGAAGA AGGACATCAT TGGTGCCAGT 
GATCCATACG TCAAACTCTC CCTGTATGTC GCTGATGAAA ACCGCGAGCT TGCCTTAGTA 
CAAACGAAAA CCATCAAAAA GACTCTTAAT CCCAAATGGA ACGAGGAATT CTATTTCAGA 
GTCTGCCCAC AAAACCACAG GCTTCTTTTC GAGGTGTTTG ACGAAAACCG CTTGACCAGA 
GATGACTTCC TGGGGCAGGT GGACGTGCCT CTTACACACT TACCGACTGA AGACCCTGCC 
ATGGAGCGTC CATACACGTT TAAAGACTTT CTCCTGCGTC CAAGAAGTCA TAAATCTCGG 
GTGAAAGGTT ACCTGAGGCT CAAGATGGCC TACCTGCCAA AGAATGGAGG ACATGAGGAG 
GAAAGCGGTG AAATGAGAGA GGAGGCTGAG GGTTGGGAAG TGACAGACTC CACAGATCCA 
GGATCTCAGA GACCTCAGCA GCTCCTGCCT CCTCTTCCTC CGGGCTGGGA AGAGAAAGTG 
GATAACCTGG GACGGACGTA CTATGTGAAC CACAACAACC GCACCACACA GTGGAAGAGG 
CCCAGCAGTG TGGATGTGGT TTCAGAAACG GAAAATGATA ATCAATTGCG GCAGATTAAC 
CAGGAAGCCC ACAGAGTTTT CCGCTCTCGA CGGCACATTA GCGAAGACCT GGAAAATGAG 
CAGCTGGACA TCAGGGACAT AGACGACTCA TGGGAGCCCA TATCAGAGGA AGATCCGACT 
TTATTAACAG ATGGTCTGAA CCATTCTCTG TCGGGACCCT CCTCTCTGGC AATGCCCCTC 
TCCAGCACTC CAGACTTCTC AGATGAGATC AGCCTTAGAT TGTCTCTCGC TCCAGACACC 
AACGGAGAAA TCCCGGGACC CAGCTCTGCT GTGCAGAGTC ATCTTTCCTC TCGGCTACGC 
TCCTCTAGTA TGACTGATGG AGTCAGTGAT CAAGCTCAAC CTCCCACACC AACGAGCTCC 
CAGACCCGAC GAACAAGAGC ACAAACCGTC ACAGGCGTTG AGGACACCAT GTCTCCTTCG 
GCCTCTTACG CCTTGACCAC GCCAGGCCTT CCACCAGGCT GGGAGGAACG CAAAGACCCC 
AAGGGACGAA CGTACTACGT TAATCATAAC AACCGTAGCA CCACATGGAC AAGGCCCATT 
CTGCAGCAGC ACACTGAGGA TGGAGCCAGC ACCTCGGTTG CAGCAGTAGG GGGCGCTACA 
GCAGCTACAC CCCCTGCTTC AACCCCTTCC ACCTCCAGCG GCCATCTAAG CGAACCACAA 
GTGCGCAGAC CCCGTAGCCT CAGTTCCCCT ACCGTCACTC TCTCTTCCCC ACTAGAGGGA 
GCCAATAACG TCCCTATACG GCGAGCCGTA AAGGACACAT TCTCCAACCC TCAGTCTCCA 
CAGCCTTCGC CCTACAGCTC ACCTAAAACC CAGCATAAAG CCAACCAGAG CTTCCTGCCA 
CCGGGATGGG AAATGAGGAT CGCACCCAAC GGACGGCCGT TCTTCATCGA CCACAACAGC 
AGAACCACCA CATGGGAGGA TCCCCGCTTG AAGTATCCCG TTCACATGAG GACAAAAGCG 
TCTCTAGACC CGGGCGACCT CGGCCCACTT CCTCCTGGGT GGGAGGAACG AGTGCATGCT 
GATGGAAGAA CCTTTTACAT CGATCACAAC AACAAAAAAA CACAGTGGGA AGACCCTCGT 
CTCCAGAGTC CTGCCATAAC TGGACCCGCG GTGCCGTATT CAAGAGAATT CAAGCAGAAA 
TACGACTACT TCAGGAAGAA GTTAAAGAAA CCGGCAGATA TCCCCAACCG CTTTGAGATG 
AAGCTGCACA GGAATAACAT CTTTGAGGAG TCCTACAGAC GAATCATGTC CCTCAAGAGA 
CCTGACAGCC TGAAGGCCCG TCTGTGGATT GAGTTTGAGT CAGAGAAGGG TCTGGATTAT 
GGCGGTGTGG CCCGAGAATG GTTCTTCCTG CTCTCTAAGG AGATGTTCAA CCCTTATTAT 
GGGCTGTTCG AGTACTCGGC CACAGATAAC TACACCCTTC AGATCAATCC CAACTCTGGC 
CTGTGTAATG AGGATCACCT CTCATACTTC AAGTTCATTG GTCGAGTGGC CGGCATGGCT 
GTTTATCATG GCAAACTCTT GGACGGTTTC TTCATTCGAC CGTTTTATAA GATGATGCTG 
GGCAAACAGA TCACACTGAA TGACATGGAG TCAGTGGACA GCGAATACTA CAACTCCCTG 
AAGTGGATTC TGGAAAACGA TCCCACAGAA TTAGACTTAC GGTTTTGTAT TGATGAGGAC 
AACTTTGGAC AGACATATCA GGTAGATTTG AAGCCAAGCG GATCAGACAT GGTTGTAACC 
AACGACAACA AGAAGGAATA CATCGATCTG GTGATTCAGT GGAGGTTTGT GAACAGGGTC 
CAGAAACAGA TGAACGCCTT TTTGGAAGGC TTCACTGAAC TCATTCCCAT CGACTTAATA 
AAGATTTTTG ATGAAAACGA ACTGGAGCTG TTGATGTGTG GTCTGGGAGA TGTGGATGTC 
AATGACTGGA GGCAACACAC TGTCTATAAG AACGGCTACT GTCCAAATCA CCCCGTCATC 
CAGTGGTTCT GGAAGGCTGT GTTGTTAATG GATGCAGAAA AGCGCATCAG GCTTCTACAG 
TTTGTCACTG GAACATCCAG AGTGCCAATG AACGGCTTTG CTGAACTCTA CGGTTCCAAT 
GGGCCGCAGT TGTTTACTAT TGAGCAGTGG GGAACTCCTG ACAAACTCCC TCGGGCTCAC 
ACCTGCTTTA ATCGCTTGGA CCTCCCAATG TATGAAACGT TTGAAGACCT GCGAGAGAAG 
CTTCTGATGG CGGTGGAAAA CGCTCAAGGC TTTGAGGGCG TCGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nedd4l ( XM_682997.7 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_688089.3 Danio rerio neural precursor cell expressed, developmentally down-regulated 4-like (nedd4l), transcript variant X15, mRNA.

Price & Availability↑

CloneID	ODa25661
Clone ID Related Accession (Same CDS sequence)	XM_682997.7
Accession Version	XM_682997.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2985bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase NEDD4-like isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 24, 2014 this sequence version replaced XM_682997.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTACGA ATTTTGAACC CATTTATGGA CTTTCAGAGG ATGAAAACGA ATCTCGTATT 
CTTCGCGTCA AGGTCATTGC TGGCATTGAT CTTGCGAAGA AGGACATCAT TGGTGCCAGT 
GATCCATACG TCAAACTCTC CCTGTATGTC GCTGATGAAA ACCGCGAGCT TGCCTTAGTA 
CAAACGAAAA CCATCAAAAA GACTCTTAAT CCCAAATGGA ACGAGGAATT CTATTTCAGA 
GTCTGCCCAC AAAACCACAG GCTTCTTTTC GAGGTGTTTG ACGAAAACCG CTTGACCAGA 
GATGACTTCC TGGGGCAGGT GGACGTGCCT CTTACACACT TACCGACTGA AGACCCTGCC 
ATGGAGCGTC CATACACGTT TAAAGACTTT CTCCTGCGTC CAAGAAGTCA TAAATCTCGG 
GTGAAAGGTT ACCTGAGGCT CAAGATGGCC TACCTGCCAA AGAATGGAGG ACATGAGGAG 
GAAAGCGGTG AAATGAGAGA GGAGGCTGAG GGTTGGGAAG TGACAGACTC CACAGATCCA 
GGATCTCAGA GACCTCAGCA GCTCCTGCCT CCTCTTCCTC CGGGCTGGGA AGAGAAAGTG 
GATAACCTGG GACGGACGTA CTATGTGAAC CACAACAACC GCACCACACA GTGGAAGAGG 
CCCAGCAGTG TGGATGTGGT TTCAGAAACG GAAAATGATA ATCAATTGCG GCAGATTAAC 
CAGGAAGCCC ACAGAGTTTT CCGCTCTCGA CGGCACATTA GCGAAGACCT GGAAAATGAG 
CAGCTGGACA TCAGGGACAT AGACGACTCA TGGGAGCCCA TATCAGAGGA AGATCCGACT 
TTATTAACAG ATGGTCTGAA CCATTCTCTG TCGGGACCCT CCTCTCTGGC AATGCCCCTC 
TCCAGCACTC CAGACTTCTC AGATGAGATC AGCCTTAGAT TGTCTCTCGC TCCAGACACC 
AACGGAGAAA TCCCGGGACC CAGCTCTGCT GTGCAGAGTC ATCTTTCCTC TCGGCTACGC 
TCCTCTAGTA TGACTGATGG AGTCAGTGAT CAAGCTCAAC CTCCCACACC AACGAGCTCC 
CAGACCCGAC GAACAAGAGC ACAAACCGTC ACAGGCGTTG AGGACACCAT GTCTCCTTCG 
GCCTCTTACG CCTTGACCAC GCCAGGCCTT CCACCAGGCT GGGAGGAACG CAAAGACCCC 
AAGGGACGAA CGTACTACGT TAATCATAAC AACCGTAGCA CCACATGGAC AAGGCCCATT 
CTGCAGCACA CTGAGGATGG AGCCAGCACC TCGGTTGCAG CAGTAGGGGG CGCTACAGCA 
GCTACACCCC CTGCTTCAAC CCCTTCCACC TCCAGCGGCC ATCTAAGCGA ACCACAAGTG 
CGCAGACCCC GTAGCCTCAG TTCCCCTACC GTCACTCTCT CTTCCCCACT AGAGGGAGCC 
AATAACGTCC CTATACGGCG AGCCGTAAAG GACACATTCT CCAACCCTCA GTCTCCACAG 
CCTTCGCCCT ACAGCTCACC TAAAACCCAG CATAAAGCCA ACCAGAGCTT CCTGCCACCG 
GGATGGGAAA TGAGGATCGC ACCCAACGGA CGGCCGTTCT TCATCGACCA CAACAGCAGA 
ACCACCACAT GGGAGGATCC CCGCTTGAAG TATCCCGTTC ACATGAGGAC AAAAGCGTCT 
CTAGACCCGG GCGACCTCGG CCCACTTCCT CCTGGGTGGG AGGAACGAGT GCATGCTGAT 
GGAAGAACCT TTTACATCGA TCACAACAAC AAAAAAACAC AGTGGGAAGA CCCTCGTCTC 
CAGAGTCCTG CCATAACTGG ACCCGCGGTG CCGTATTCAA GAGAATTCAA GCAGAAATAC 
GACTACTTCA GGAAGAAGTT AAAGAAACCG GCAGATATCC CCAACCGCTT TGAGATGAAG 
CTGCACAGGA ATAACATCTT TGAGGAGTCC TACAGACGAA TCATGTCCCT CAAGAGACCT 
GACAGCCTGA AGGCCCGTCT GTGGATTGAG TTTGAGTCAG AGAAGGGTCT GGATTATGGC 
GGTGTGGCCC GAGAATGGTT CTTCCTGCTC TCTAAGGAGA TGTTCAACCC TTATTATGGG 
CTGTTCGAGT ACTCGGCCAC AGATAACTAC ACCCTTCAGA TCAATCCCAA CTCTGGCCTG 
TGTAATGAGG ATCACCTCTC ATACTTCAAG TTCATTGGTC GAGTGGCCGG CATGGCTGTT 
TATCATGGCA AACTCTTGGA CGGTTTCTTC ATTCGACCGT TTTATAAGAT GATGCTGGGC 
AAACAGATCA CACTGAATGA CATGGAGTCA GTGGACAGCG AATACTACAA CTCCCTGAAG 
TGGATTCTGG AAAACGATCC CACAGAATTA GACTTACGGT TTTGTATTGA TGAGGACAAC 
TTTGGACAGA CATATCAGGT AGATTTGAAG CCAAGCGGAT CAGACATGGT TGTAACCAAC 
GACAACAAGA AGGAATACAT CGATCTGGTG ATTCAGTGGA GGTTTGTGAA CAGGGTCCAG 
AAACAGATGA ACGCCTTTTT GGAAGGCTTC ACTGAACTCA TTCCCATCGA CTTAATAAAG 
ATTTTTGATG AAAACGAACT GGAGCTGTTG ATGTGTGGTC TGGGAGATGT GGATGTCAAT 
GACTGGAGGC AACACACTGT CTATAAGAAC GGCTACTGTC CAAATCACCC CGTCATCCAG 
TGGTTCTGGA AGGCTGTGTT GTTAATGGAT GCAGAAAAGC GCATCAGGCT TCTACAGTTT 
GTCACTGGAA CATCCAGAGT GCCAATGAAC GGCTTTGCTG AACTCTACGG TTCCAATGGG 
CCGCAGTTGT TTACTATTGA GCAGTGGGGA ACTCCTGACA AACTCCCTCG GGCTCACACC 
TGCTTTAATC GCTTGGACCT CCCAATGTAT GAAACGTTTG AAGACCTGCG AGAGAAGCTT 
CTGATGGCGG TGGAAAACGC TCAAGGCTTT GAGGGCGTCG ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_688089.3
CDS	304..3288
Translation	MATNFEPIYGLSEDENESRILRVKVIAGIDLAKKDIIGASDPYVKLSLYVADENRELALVQTKTIKKTLNPKWNEEFYFRVCPQNHRLLFEVFDENRLTRDDFLGQVDVPLTHLPTEDPAMERPYTFKDFLLRPRSHKSRVKGYLRLKMAYLPKNGGHEEESGEMREEAEGWEVTDSTDPGSQRPQQLLPPLPPGWEEKVDNLGRTYYVNHNNRTTQWKRPSSVDVVSETENDNQLRQINQEAHRVFRSRRHISEDLENEQLDIRDIDDSWEPISEEDPTLLTDGLNHSLSGPSSLAMPLSSTPDFSDEISLRLSLAPDTNGEIPGPSSAVQSHLSSRLRSSSMTDGVSDQAQPPTPTSSQTRRTRAQTVTGVEDTMSPSASYALTTPGLPPGWEERKDPKGRTYYVNHNNRSTTWTRPILQHTEDGASTSVAAVGGATAATPPASTPSTSSGHLSEPQVRRPRSLSSPTVTLSSPLEGANNVPIRRAVKDTFSNPQSPQPSPYSSPKTQHKANQSFLPPGWEMRIAPNGRPFFIDHNSRTTTWEDPRLKYPVHMRTKASLDPGDLGPLPPGWEERVHADGRTFYIDHNNKKTQWEDPRLQSPAITGPAVPYSREFKQKYDYFRKKLKKPADIPNRFEMKLHRNNIFEESYRRIMSLKRPDSLKARLWIEFESEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFKFIGRVAGMAVYHGKLLDGFFIRPFYKMMLGKQITLNDMESVDSEYYNSLKWILENDPTELDLRFCIDEDNFGQTYQVDLKPSGSDMVVTNDNKKEYIDLVIQWRFVNRVQKQMNAFLEGFTELIPIDLIKIFDENELELLMCGLGDVDVNDWRQHTVYKNGYCPNHPVIQWFWKAVLLMDAEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGTPDKLPRAHTCFNRLDLPMYETFEDLREKLLMAVENAQGFEGVD

Target ORF information:

RefSeq Version	XM_682997.7
Organism	Danio rerio(zebrafish)
Definition	Danio rerio neural precursor cell expressed, developmentally down-regulated 4-like (nedd4l), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_682997.7

ORF Insert Sequence:

ATGGCTACGA ATTTTGAACC CATTTATGGA CTTTCAGAGG ATGAAAACGA ATCTCGTATT 
CTTCGCGTCA AGGTCATTGC TGGCATTGAT CTTGCGAAGA AGGACATCAT TGGTGCCAGT 
GATCCATACG TCAAACTCTC CCTGTATGTC GCTGATGAAA ACCGCGAGCT TGCCTTAGTA 
CAAACGAAAA CCATCAAAAA GACTCTTAAT CCCAAATGGA ACGAGGAATT CTATTTCAGA 
GTCTGCCCAC AAAACCACAG GCTTCTTTTC GAGGTGTTTG ACGAAAACCG CTTGACCAGA 
GATGACTTCC TGGGGCAGGT GGACGTGCCT CTTACACACT TACCGACTGA AGACCCTGCC 
ATGGAGCGTC CATACACGTT TAAAGACTTT CTCCTGCGTC CAAGAAGTCA TAAATCTCGG 
GTGAAAGGTT ACCTGAGGCT CAAGATGGCC TACCTGCCAA AGAATGGAGG ACATGAGGAG 
GAAAGCGGTG AAATGAGAGA GGAGGCTGAG GGTTGGGAAG TGACAGACTC CACAGATCCA 
GGATCTCAGA GACCTCAGCA GCTCCTGCCT CCTCTTCCTC CGGGCTGGGA AGAGAAAGTG 
GATAACCTGG GACGGACGTA CTATGTGAAC CACAACAACC GCACCACACA GTGGAAGAGG 
CCCAGCAGTG TGGATGTGGT TTCAGAAACG GAAAATGATA ATCAATTGCG GCAGATTAAC 
CAGGAAGCCC ACAGAGTTTT CCGCTCTCGA CGGCACATTA GCGAAGACCT GGAAAATGAG 
CAGCTGGACA TCAGGGACAT AGACGACTCA TGGGAGCCCA TATCAGAGGA AGATCCGACT 
TTATTAACAG ATGGTCTGAA CCATTCTCTG TCGGGACCCT CCTCTCTGGC AATGCCCCTC 
TCCAGCACTC CAGACTTCTC AGATGAGATC AGCCTTAGAT TGTCTCTCGC TCCAGACACC 
AACGGAGAAA TCCCGGGACC CAGCTCTGCT GTGCAGAGTC ATCTTTCCTC TCGGCTACGC 
TCCTCTAGTA TGACTGATGG AGTCAGTGAT CAAGCTCAAC CTCCCACACC AACGAGCTCC 
CAGACCCGAC GAACAAGAGC ACAAACCGTC ACAGGCGTTG AGGACACCAT GTCTCCTTCG 
GCCTCTTACG CCTTGACCAC GCCAGGCCTT CCACCAGGCT GGGAGGAACG CAAAGACCCC 
AAGGGACGAA CGTACTACGT TAATCATAAC AACCGTAGCA CCACATGGAC AAGGCCCATT 
CTGCAGCACA CTGAGGATGG AGCCAGCACC TCGGTTGCAG CAGTAGGGGG CGCTACAGCA 
GCTACACCCC CTGCTTCAAC CCCTTCCACC TCCAGCGGCC ATCTAAGCGA ACCACAAGTG 
CGCAGACCCC GTAGCCTCAG TTCCCCTACC GTCACTCTCT CTTCCCCACT AGAGGGAGCC 
AATAACGTCC CTATACGGCG AGCCGTAAAG GACACATTCT CCAACCCTCA GTCTCCACAG 
CCTTCGCCCT ACAGCTCACC TAAAACCCAG CATAAAGCCA ACCAGAGCTT CCTGCCACCG 
GGATGGGAAA TGAGGATCGC ACCCAACGGA CGGCCGTTCT TCATCGACCA CAACAGCAGA 
ACCACCACAT GGGAGGATCC CCGCTTGAAG TATCCCGTTC ACATGAGGAC AAAAGCGTCT 
CTAGACCCGG GCGACCTCGG CCCACTTCCT CCTGGGTGGG AGGAACGAGT GCATGCTGAT 
GGAAGAACCT TTTACATCGA TCACAACAAC AAAAAAACAC AGTGGGAAGA CCCTCGTCTC 
CAGAGTCCTG CCATAACTGG ACCCGCGGTG CCGTATTCAA GAGAATTCAA GCAGAAATAC 
GACTACTTCA GGAAGAAGTT AAAGAAACCG GCAGATATCC CCAACCGCTT TGAGATGAAG 
CTGCACAGGA ATAACATCTT TGAGGAGTCC TACAGACGAA TCATGTCCCT CAAGAGACCT 
GACAGCCTGA AGGCCCGTCT GTGGATTGAG TTTGAGTCAG AGAAGGGTCT GGATTATGGC 
GGTGTGGCCC GAGAATGGTT CTTCCTGCTC TCTAAGGAGA TGTTCAACCC TTATTATGGG 
CTGTTCGAGT ACTCGGCCAC AGATAACTAC ACCCTTCAGA TCAATCCCAA CTCTGGCCTG 
TGTAATGAGG ATCACCTCTC ATACTTCAAG TTCATTGGTC GAGTGGCCGG CATGGCTGTT 
TATCATGGCA AACTCTTGGA CGGTTTCTTC ATTCGACCGT TTTATAAGAT GATGCTGGGC 
AAACAGATCA CACTGAATGA CATGGAGTCA GTGGACAGCG AATACTACAA CTCCCTGAAG 
TGGATTCTGG AAAACGATCC CACAGAATTA GACTTACGGT TTTGTATTGA TGAGGACAAC 
TTTGGACAGA CATATCAGGT AGATTTGAAG CCAAGCGGAT CAGACATGGT TGTAACCAAC 
GACAACAAGA AGGAATACAT CGATCTGGTG ATTCAGTGGA GGTTTGTGAA CAGGGTCCAG 
AAACAGATGA ACGCCTTTTT GGAAGGCTTC ACTGAACTCA TTCCCATCGA CTTAATAAAG 
ATTTTTGATG AAAACGAACT GGAGCTGTTG ATGTGTGGTC TGGGAGATGT GGATGTCAAT 
GACTGGAGGC AACACACTGT CTATAAGAAC GGCTACTGTC CAAATCACCC CGTCATCCAG 
TGGTTCTGGA AGGCTGTGTT GTTAATGGAT GCAGAAAAGC GCATCAGGCT TCTACAGTTT 
GTCACTGGAA CATCCAGAGT GCCAATGAAC GGCTTTGCTG AACTCTACGG TTCCAATGGG 
CCGCAGTTGT TTACTATTGA GCAGTGGGGA ACTCCTGACA AACTCCCTCG GGCTCACACC 
TGCTTTAATC GCTTGGACCT CCCAATGTAT GAAACGTTTG AAGACCTGCG AGAGAAGCTT 
CTGATGGCGG TGGAAAACGC TCAAGGCTTT GAGGGCGTCG ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nedd4l ( XM_021469012.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324687.1 Danio rerio neural precursor cell expressed, developmentally down-regulated 4-like (nedd4l), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa151011
Clone ID Related Accession (Same CDS sequence)	XM_021469012.1
Accession Version	XM_021469012.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3840bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase NEDD4-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCACC GGCTTCGGTT GTATTTTGGA TCTGGTCGCA GTAACACTGC TCCAGAGTTG 
GAAGAGCTGA ATGGGACAGT GGAGGAGGAA GGTGACATAA GCTACAACAG TACAGGAGCC 
ATGGTGGTCC ATGCACAACC ACGTAACGGA GGACATGCAG AAAGTCCAGA ACCAGGCACA 
GTTGTTGGTT CGGATGCCTC TCTAAAGCGA AGCTCTTCCA TGTTCATCCC ACAGCTCCAG 
GGTCAAGCTG AGTCACGGAC AACCAAGAGC ACCTCTGTTC AAATATCTCT TCAACGCACA 
ACTGCCCCTC ATGAAGATGC ATTACCCTTT GATCCACCTC CAGCGTACCC CCAGGATCCA 
GCTCCACCAT ACACTGAACC TGGTGAAAGT TTGCCCTTAC CTCCACCTCC AACCTACTAC 
AAATCCTGCC AAAATGTGGA ACAAACTCAA AAAGATACTG AACCTAAGAG GCAAGTCTTT 
TGTGTCCGGC CATGTGATGG TCAAAATGGT TATACCCATG GAAATCCTCA AGGGATCACT 
ATTGGAGGTG TCTGTATTAG AAGAAGATCT GCAGATGGCT CGGATGTCCA GCAGTTCAGG 
ATTATGCCAT ATAGCGAGGA TGGGCACAAT GGGCACAATT GGTCTGTCCA GCAATGCTCA 
GACAGAGGTC CTGGTGCACA AAGACTAATT TTTCAGCTCC AAAAGGACCA AGGACGGCAA 
CCAAGCTCTA CCTCTCAGCC TGACGTGAGT GATTTAGGCA TCACAGGTTC TAAAGGTTCC 
CGCTTGGTGC GGTACCCCAG GATTCGACTT GAGCGGAGCA CGTCACAGCC ATTGCAGCCT 
AGAGGAGCTC CTTCACGCCT TGGATCAATG CCAAACCTTC ATGCTCCTAT CTCAGAAATG 
GAAACGGAGA GTGATTTTAT TGAGAATGGT GCTCAAGGTT GCACTTTCAA AATTAGGAAG 
GAACAGAATT CCCAACAACC GAAGTTTAAG ATTTATTTCA GCCAGGGCGG CGAGCACAGT 
CCCATCCTCG AAAGGAATAT TAGCATTGGA AAGGCGCCTA CCTGCAGCAA TCCACAGGCT 
CACATTGGTC TTGATCGGCC CAAGAACGGT CATTTCAACA ATGGTCAGAC CAGAGATGAC 
TTCCTGGGGC AGGTGGACGT GCCTCTTACA CACTTACCGA CTGAAGACCC TGCCATGGAG 
CGTCCATACA CGTTTAAAGA CTTTCTCCTG CGTCCAAGAA GTCATAAATC TCGGGTGAAA 
GGTTACCTGA GGCTCAAGAT GGCCTACCTG CCAAAGAATG GAGGACATGA GGAGGAAAGC 
GGTGAAATGA GAGAGGAGGC TGAGGGTTGG GAAGTGACAG ACTCCACAGA TCCAGGATCT 
CAGAGACCTC AGCAGCTCCT GCCTCCTCTT CCTCCGGGCT GGGAAGAGAA AGTGGATAAC 
CTGGGACGGA CGTACTATGT GAACCACAAC AACCGCACCA CACAGTGGAA GAGGCCCAGC 
AGTGTGGATG TGGTTTCAGA AACGGAAAAT GATAATCAAT TGCGGCAGAT TAACCAGGAA 
GCCCACAGAG TTTTCCGCTC TCGACGGCAC ATTAGCGAAG ACCTGGAAAA TGAGCAGCTG 
GACATCAGGG ACATAGACGA CTCATGGGAG CCCATATCAG AGGAAGATCC GACTTTATTA 
ACAGATGGTC TGAACCATTC TCTGTCGGGA CCCTCCTCTC TGGCAATGCC CCTCTCCAGC 
ACTCCAGACT TCTCAGATGA GATCAGCCTT AGATTGTCTC TCGCTCCAGA CACCAACGGA 
GAAATCCCGG GACCCAGCTC TGCTGTGCAG AGTCATCTTT CCTCTCGGCT ACGCTCCTCT 
AGTATGACTG ATGGAGTCAG TGATCAAGCT CAACCTCCCA CACCAACGCA GAGCTCCCAG 
ACCCGACGAA CAAGAGCACA AACCGTCACA GGCGTTGAGG ACACCATGTC TCCTTCGGCC 
TCTTACGCCT TGACCACGCC AGGCCTTCCA CCAGGCTGGG AGGAACGCAA AGACCCCAAG 
GGACGAACGT ACTACGTTAA TCATAACAAC CGTAGCACCA CATGGACAAG GCCCATTCTG 
CAGCAGCACA CTGAGGATGG AGCCAGCACC TCGGTTGCAG CAGTAGGGGG CGCTACAGCA 
GCTACACCCC CTGCTTCAAC CCCTTCCACC TCCAGCGGCC ATCTAAGCGA ACCACAAGTG 
CGCAGACCCC GTAGCCTCAG TTCCCCTACC GTCACTCTCT CTTCCCCACT AGAGGGAGCC 
AATAACGTCC CTATACGGCG AGCCGTAAAG GACACATTCT CCAACCCTCA GTCTCCACAG 
CCTTCGCCCT ACAGCTCACC TAAAACCCAG CATAAAGCCA ACCAGAGCTT CCTGCCACCG 
GGATGGGAAA TGAGGATCGC ACCCAACGGA CGGCCGTTCT TCATCGACCA CAACAGCAGA 
ACCACCACAT GGGAGGATCC CCGCTTGAAG TATCCCGTTC ACATGAGGAC AAAAGCGTCT 
CTAGACCCGG GCGACCTCGG CCCACTTCCT AATCTTCCAG AGGAGCCTGG GTGGGAGGAA 
CGAGTGCATG CTGATGGAAG AACCTTTTAC ATCGATCACA ACAACAAAAA AACACAGTGG 
GAAGACCCTC GTCTCCAGAG TCCTGCCATA ACTGGACCCG CGGTGCCGTA TTCAAGAGAA 
TTCAAGCAGA AATACGACTA CTTCAGGAAG AAGTTAAAGA AACCGGCAGA TATCCCCAAC 
CGCTTTGAGA TGAAGCTGCA CAGGAATAAC ATCTTTGAGG AGTCCTACAG ACGAATCATG 
TCCCTCAAGA GACCTGACAG CCTGAAGGCC CGTCTGTGGA TTGAGTTTGA GTCAGAGAAG 
GGTCTGGATT ATGGCGGTGT GGCCCGAGAA TGGTTCTTCC TGCTCTCTAA GGAGATGTTC 
AACCCTTATT ATGGGCTGTT CGAGTACTCG GCCACAGATA ACTACACCCT TCAGATCAAT 
CCCAACTCTG GCCTGTGTAA TGAGGATCAC CTCTCATACT TCAAGTTCAT TGGTCGAGTG 
GCCGGCATGG CTGTTTATCA TGGCAAACTC TTGGACGGTT TCTTCATTCG ACCGTTTTAT 
AAGATGATGC TGGGCAAACA GATCACACTG AATGACATGG AGTCAGTGGA CAGCGAATAC 
TACAACTCCC TGAAGTGGAT TCTGGAAAAC GATCCCACAG AATTAGACTT ACGGTTTTGT 
ATTGATGAGG ACAACTTTGG ACAGACATAT CAGGTAGATT TGAAGCCAAG CGGATCAGAC 
ATGGTTGTAA CCAACGACAA CAAGAAGGAA TACATCGATC TGGTGATTCA GTGGAGGTTT 
GTGAACAGGG TCCAGAAACA GATGAACGCC TTTTTGGAAG GCTTCACTGA ACTCATTCCC 
ATCGACTTAA TAAAGATTTT TGATGAAAAC GAACTGGAGC TGTTGATGTG TGGTCTGGGA 
GATGTGGATG TCAATGACTG GAGGCAACAC ACTGTCTATA AGAACGGCTA CTGTCCAAAT 
CACCCCGTCA TCCAGTGGTT CTGGAAGGCT GTGTTGTTAA TGGATGCAGA AAAGCGCATC 
AGGCTTCTAC AGTTTGTCAC TGGAACATCC AGAGTGCCAA TGAACGGCTT TGCTGAACTC 
TACGGTTCCA ATGGGCCGCA GTTGTTTACT ATTGAGCAGT GGGGAACTCC TGACAAACTC 
CCTCGGGCTC ACACCTGCTT TAATCGCTTG GACCTCCCAA TGTATGAAAC GTTTGAAGAC 
CTGCGAGAGA AGCTTCTGAT GGCGGTGGAA AACGCTCAAG GCTTTGAGGG CGTCGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324687.1
CDS	342..4181
Translation	MSHRLRLYFGSGRSNTAPELEELNGTVEEEGDISYNSTGAMVVHAQPRNGGHAESPEPGTVVGSDASLKRSSSMFIPQLQGQAESRTTKSTSVQISLQRTTAPHEDALPFDPPPAYPQDPAPPYTEPGESLPLPPPPTYYKSCQNVEQTQKDTEPKRQVFCVRPCDGQNGYTHGNPQGITIGGVCIRRRSADGSDVQQFRIMPYSEDGHNGHNWSVQQCSDRGPGAQRLIFQLQKDQGRQPSSTSQPDVSDLGITGSKGSRLVRYPRIRLERSTSQPLQPRGAPSRLGSMPNLHAPISEMETESDFIENGAQGCTFKIRKEQNSQQPKFKIYFSQGGEHSPILERNISIGKAPTCSNPQAHIGLDRPKNGHFNNGQTRDDFLGQVDVPLTHLPTEDPAMERPYTFKDFLLRPRSHKSRVKGYLRLKMAYLPKNGGHEEESGEMREEAEGWEVTDSTDPGSQRPQQLLPPLPPGWEEKVDNLGRTYYVNHNNRTTQWKRPSSVDVVSETENDNQLRQINQEAHRVFRSRRHISEDLENEQLDIRDIDDSWEPISEEDPTLLTDGLNHSLSGPSSLAMPLSSTPDFSDEISLRLSLAPDTNGEIPGPSSAVQSHLSSRLRSSSMTDGVSDQAQPPTPTQSSQTRRTRAQTVTGVEDTMSPSASYALTTPGLPPGWEERKDPKGRTYYVNHNNRSTTWTRPILQQHTEDGASTSVAAVGGATAATPPASTPSTSSGHLSEPQVRRPRSLSSPTVTLSSPLEGANNVPIRRAVKDTFSNPQSPQPSPYSSPKTQHKANQSFLPPGWEMRIAPNGRPFFIDHNSRTTTWEDPRLKYPVHMRTKASLDPGDLGPLPNLPEEPGWEERVHADGRTFYIDHNNKKTQWEDPRLQSPAITGPAVPYSREFKQKYDYFRKKLKKPADIPNRFEMKLHRNNIFEESYRRIMSLKRPDSLKARLWIEFESEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFKFIGRVAGMAVYHGKLLDGFFIRPFYKMMLGKQITLNDMESVDSEYYNSLKWILENDPTELDLRFCIDEDNFGQTYQVDLKPSGSDMVVTNDNKKEYIDLVIQWRFVNRVQKQMNAFLEGFTELIPIDLIKIFDENELELLMCGLGDVDVNDWRQHTVYKNGYCPNHPVIQWFWKAVLLMDAEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGTPDKLPRAHTCFNRLDLPMYETFEDLREKLLMAVENAQGFEGVD

Target ORF information:

RefSeq Version	XM_021469012.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio neural precursor cell expressed, developmentally down-regulated 4-like (nedd4l), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021469012.1

ORF Insert Sequence:

ATGTCTCACC GGCTTCGGTT GTATTTTGGA TCTGGTCGCA GTAACACTGC TCCAGAGTTG 
GAAGAGCTGA ATGGGACAGT GGAGGAGGAA GGTGACATAA GCTACAACAG TACAGGAGCC 
ATGGTGGTCC ATGCACAACC ACGTAACGGA GGACATGCAG AAAGTCCAGA ACCAGGCACA 
GTTGTTGGTT CGGATGCCTC TCTAAAGCGA AGCTCTTCCA TGTTCATCCC ACAGCTCCAG 
GGTCAAGCTG AGTCACGGAC AACCAAGAGC ACCTCTGTTC AAATATCTCT TCAACGCACA 
ACTGCCCCTC ATGAAGATGC ATTACCCTTT GATCCACCTC CAGCGTACCC CCAGGATCCA 
GCTCCACCAT ACACTGAACC TGGTGAAAGT TTGCCCTTAC CTCCACCTCC AACCTACTAC 
AAATCCTGCC AAAATGTGGA ACAAACTCAA AAAGATACTG AACCTAAGAG GCAAGTCTTT 
TGTGTCCGGC CATGTGATGG TCAAAATGGT TATACCCATG GAAATCCTCA AGGGATCACT 
ATTGGAGGTG TCTGTATTAG AAGAAGATCT GCAGATGGCT CGGATGTCCA GCAGTTCAGG 
ATTATGCCAT ATAGCGAGGA TGGGCACAAT GGGCACAATT GGTCTGTCCA GCAATGCTCA 
GACAGAGGTC CTGGTGCACA AAGACTAATT TTTCAGCTCC AAAAGGACCA AGGACGGCAA 
CCAAGCTCTA CCTCTCAGCC TGACGTGAGT GATTTAGGCA TCACAGGTTC TAAAGGTTCC 
CGCTTGGTGC GGTACCCCAG GATTCGACTT GAGCGGAGCA CGTCACAGCC ATTGCAGCCT 
AGAGGAGCTC CTTCACGCCT TGGATCAATG CCAAACCTTC ATGCTCCTAT CTCAGAAATG 
GAAACGGAGA GTGATTTTAT TGAGAATGGT GCTCAAGGTT GCACTTTCAA AATTAGGAAG 
GAACAGAATT CCCAACAACC GAAGTTTAAG ATTTATTTCA GCCAGGGCGG CGAGCACAGT 
CCCATCCTCG AAAGGAATAT TAGCATTGGA AAGGCGCCTA CCTGCAGCAA TCCACAGGCT 
CACATTGGTC TTGATCGGCC CAAGAACGGT CATTTCAACA ATGGTCAGAC CAGAGATGAC 
TTCCTGGGGC AGGTGGACGT GCCTCTTACA CACTTACCGA CTGAAGACCC TGCCATGGAG 
CGTCCATACA CGTTTAAAGA CTTTCTCCTG CGTCCAAGAA GTCATAAATC TCGGGTGAAA 
GGTTACCTGA GGCTCAAGAT GGCCTACCTG CCAAAGAATG GAGGACATGA GGAGGAAAGC 
GGTGAAATGA GAGAGGAGGC TGAGGGTTGG GAAGTGACAG ACTCCACAGA TCCAGGATCT 
CAGAGACCTC AGCAGCTCCT GCCTCCTCTT CCTCCGGGCT GGGAAGAGAA AGTGGATAAC 
CTGGGACGGA CGTACTATGT GAACCACAAC AACCGCACCA CACAGTGGAA GAGGCCCAGC 
AGTGTGGATG TGGTTTCAGA AACGGAAAAT GATAATCAAT TGCGGCAGAT TAACCAGGAA 
GCCCACAGAG TTTTCCGCTC TCGACGGCAC ATTAGCGAAG ACCTGGAAAA TGAGCAGCTG 
GACATCAGGG ACATAGACGA CTCATGGGAG CCCATATCAG AGGAAGATCC GACTTTATTA 
ACAGATGGTC TGAACCATTC TCTGTCGGGA CCCTCCTCTC TGGCAATGCC CCTCTCCAGC 
ACTCCAGACT TCTCAGATGA GATCAGCCTT AGATTGTCTC TCGCTCCAGA CACCAACGGA 
GAAATCCCGG GACCCAGCTC TGCTGTGCAG AGTCATCTTT CCTCTCGGCT ACGCTCCTCT 
AGTATGACTG ATGGAGTCAG TGATCAAGCT CAACCTCCCA CACCAACGCA GAGCTCCCAG 
ACCCGACGAA CAAGAGCACA AACCGTCACA GGCGTTGAGG ACACCATGTC TCCTTCGGCC 
TCTTACGCCT TGACCACGCC AGGCCTTCCA CCAGGCTGGG AGGAACGCAA AGACCCCAAG 
GGACGAACGT ACTACGTTAA TCATAACAAC CGTAGCACCA CATGGACAAG GCCCATTCTG 
CAGCAGCACA CTGAGGATGG AGCCAGCACC TCGGTTGCAG CAGTAGGGGG CGCTACAGCA 
GCTACACCCC CTGCTTCAAC CCCTTCCACC TCCAGCGGCC ATCTAAGCGA ACCACAAGTG 
CGCAGACCCC GTAGCCTCAG TTCCCCTACC GTCACTCTCT CTTCCCCACT AGAGGGAGCC 
AATAACGTCC CTATACGGCG AGCCGTAAAG GACACATTCT CCAACCCTCA GTCTCCACAG 
CCTTCGCCCT ACAGCTCACC TAAAACCCAG CATAAAGCCA ACCAGAGCTT CCTGCCACCG 
GGATGGGAAA TGAGGATCGC ACCCAACGGA CGGCCGTTCT TCATCGACCA CAACAGCAGA 
ACCACCACAT GGGAGGATCC CCGCTTGAAG TATCCCGTTC ACATGAGGAC AAAAGCGTCT 
CTAGACCCGG GCGACCTCGG CCCACTTCCT AATCTTCCAG AGGAGCCTGG GTGGGAGGAA 
CGAGTGCATG CTGATGGAAG AACCTTTTAC ATCGATCACA ACAACAAAAA AACACAGTGG 
GAAGACCCTC GTCTCCAGAG TCCTGCCATA ACTGGACCCG CGGTGCCGTA TTCAAGAGAA 
TTCAAGCAGA AATACGACTA CTTCAGGAAG AAGTTAAAGA AACCGGCAGA TATCCCCAAC 
CGCTTTGAGA TGAAGCTGCA CAGGAATAAC ATCTTTGAGG AGTCCTACAG ACGAATCATG 
TCCCTCAAGA GACCTGACAG CCTGAAGGCC CGTCTGTGGA TTGAGTTTGA GTCAGAGAAG 
GGTCTGGATT ATGGCGGTGT GGCCCGAGAA TGGTTCTTCC TGCTCTCTAA GGAGATGTTC 
AACCCTTATT ATGGGCTGTT CGAGTACTCG GCCACAGATA ACTACACCCT TCAGATCAAT 
CCCAACTCTG GCCTGTGTAA TGAGGATCAC CTCTCATACT TCAAGTTCAT TGGTCGAGTG 
GCCGGCATGG CTGTTTATCA TGGCAAACTC TTGGACGGTT TCTTCATTCG ACCGTTTTAT 
AAGATGATGC TGGGCAAACA GATCACACTG AATGACATGG AGTCAGTGGA CAGCGAATAC 
TACAACTCCC TGAAGTGGAT TCTGGAAAAC GATCCCACAG AATTAGACTT ACGGTTTTGT 
ATTGATGAGG ACAACTTTGG ACAGACATAT CAGGTAGATT TGAAGCCAAG CGGATCAGAC 
ATGGTTGTAA CCAACGACAA CAAGAAGGAA TACATCGATC TGGTGATTCA GTGGAGGTTT 
GTGAACAGGG TCCAGAAACA GATGAACGCC TTTTTGGAAG GCTTCACTGA ACTCATTCCC 
ATCGACTTAA TAAAGATTTT TGATGAAAAC GAACTGGAGC TGTTGATGTG TGGTCTGGGA 
GATGTGGATG TCAATGACTG GAGGCAACAC ACTGTCTATA AGAACGGCTA CTGTCCAAAT 
CACCCCGTCA TCCAGTGGTT CTGGAAGGCT GTGTTGTTAA TGGATGCAGA AAAGCGCATC 
AGGCTTCTAC AGTTTGTCAC TGGAACATCC AGAGTGCCAA TGAACGGCTT TGCTGAACTC 
TACGGTTCCA ATGGGCCGCA GTTGTTTACT ATTGAGCAGT GGGGAACTCC TGACAAACTC 
CCTCGGGCTC ACACCTGCTT TAATCGCTTG GACCTCCCAA TGTATGAAAC GTTTGAAGAC 
CTGCGAGAGA AGCTTCTGAT GGCGGTGGAA AACGCTCAAG GCTTTGAGGG CGTCGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nedd4l ( XM_021469013.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324688.1 Danio rerio neural precursor cell expressed, developmentally down-regulated 4-like (nedd4l), transcript variant X2, mRNA.

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CloneID	ODa151012
Clone ID Related Accession (Same CDS sequence)	XM_021469013.1
Accession Version	XM_021469013.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3837bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase NEDD4-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCACC GGCTTCGGTT GTATTTTGGA TCTGGTCGCA GTAACACTGC TCCAGAGTTG 
GAAGAGCTGA ATGGGACAGT GGAGGAGGAA GGTGACATAA GCTACAACAG TACAGGAGCC 
ATGGTGGTCC ATGCACAACC ACGTAACGGA GGACATGCAG AAAGTCCAGA ACCAGGCACA 
GTTGTTGGTT CGGATGCCTC TCTAAAGCGA AGCTCTTCCA TGTTCATCCC ACAGCTCCAG 
GGTCAAGCTG AGTCACGGAC AACCAAGAGC ACCTCTGTTC AAATATCTCT TCAACGCACA 
ACTGCCCCTC ATGAAGATGC ATTACCCTTT GATCCACCTC CAGCGTACCC CCAGGATCCA 
GCTCCACCAT ACACTGAACC TGGTGAAAGT TTGCCCTTAC CTCCACCTCC AACCTACTAC 
AAATCCTGCC AAAATGTGGA ACAAACTCAA AAAGATACTG AACCTAAGAG GCAAGTCTTT 
TGTGTCCGGC CATGTGATGG TCAAAATGGT TATACCCATG GAAATCCTCA AGGGATCACT 
ATTGGAGGTG TCTGTATTAG AAGAAGATCT GCAGATGGCT CGGATGTCCA GCAGTTCAGG 
ATTATGCCAT ATAGCGAGGA TGGGCACAAT GGGCACAATT GGTCTGTCCA GCAATGCTCA 
GACAGAGGTC CTGGTGCACA AAGACTAATT TTTCAGCTCC AAAAGGACCA AGGACGGCAA 
CCAAGCTCTA CCTCTCAGCC TGACGTGAGT GATTTAGGCA TCACAGGTTC TAAAGGTTCC 
CGCTTGGTGC GGTACCCCAG GATTCGACTT GAGCGGAGCA CGTCACAGCC ATTGCAGCCT 
AGAGGAGCTC CTTCACGCCT TGGATCAATG CCAAACCTTC ATGCTCCTAT CTCAGAAATG 
GAAACGGAGA GTGATTTTAT TGAGAATGGT GCTCAAGGTT GCACTTTCAA AATTAGGAAG 
GAACAGAATT CCCAACAACC GAAGTTTAAG ATTTATTTCA GCCAGGGCGG CGAGCACAGT 
CCCATCCTCG AAAGGAATAT TAGCATTGGA AAGGCGCCTA CCTGCAGCAA TCCACAGGCT 
CACATTGGTC TTGATCGGCC CAAGAACGGT CATTTCAACA ATGGTCAGAC CAGAGATGAC 
TTCCTGGGGC AGGTGGACGT GCCTCTTACA CACTTACCGA CTGAAGACCC TGCCATGGAG 
CGTCCATACA CGTTTAAAGA CTTTCTCCTG CGTCCAAGAA GTCATAAATC TCGGGTGAAA 
GGTTACCTGA GGCTCAAGAT GGCCTACCTG CCAAAGAATG GAGGACATGA GGAGGAAAGC 
GGTGAAATGA GAGAGGAGGC TGAGGGTTGG GAAGTGACAG ACTCCACAGA TCCAGGATCT 
CAGAGACCTC AGCAGCTCCT GCCTCCTCTT CCTCCGGGCT GGGAAGAGAA AGTGGATAAC 
CTGGGACGGA CGTACTATGT GAACCACAAC AACCGCACCA CACAGTGGAA GAGGCCCAGC 
AGTGTGGATG TGGTTTCAGA AACGGAAAAT GATAATCAAT TGCGGCAGAT TAACCAGGAA 
GCCCACAGAG TTTTCCGCTC TCGACGGCAC ATTAGCGAAG ACCTGGAAAA TGAGCAGCTG 
GACATCAGGG ACATAGACGA CTCATGGGAG CCCATATCAG AGGAAGATCC GACTTTATTA 
ACAGATGGTC TGAACCATTC TCTGTCGGGA CCCTCCTCTC TGGCAATGCC CCTCTCCAGC 
ACTCCAGACT TCTCAGATGA GATCAGCCTT AGATTGTCTC TCGCTCCAGA CACCAACGGA 
GAAATCCCGG GACCCAGCTC TGCTGTGCAG AGTCATCTTT CCTCTCGGCT ACGCTCCTCT 
AGTATGACTG ATGGAGTCAG TGATCAAGCT CAACCTCCCA CACCAACGAG CTCCCAGACC 
CGACGAACAA GAGCACAAAC CGTCACAGGC GTTGAGGACA CCATGTCTCC TTCGGCCTCT 
TACGCCTTGA CCACGCCAGG CCTTCCACCA GGCTGGGAGG AACGCAAAGA CCCCAAGGGA 
CGAACGTACT ACGTTAATCA TAACAACCGT AGCACCACAT GGACAAGGCC CATTCTGCAG 
CAGCACACTG AGGATGGAGC CAGCACCTCG GTTGCAGCAG TAGGGGGCGC TACAGCAGCT 
ACACCCCCTG CTTCAACCCC TTCCACCTCC AGCGGCCATC TAAGCGAACC ACAAGTGCGC 
AGACCCCGTA GCCTCAGTTC CCCTACCGTC ACTCTCTCTT CCCCACTAGA GGGAGCCAAT 
AACGTCCCTA TACGGCGAGC CGTAAAGGAC ACATTCTCCA ACCCTCAGTC TCCACAGCCT 
TCGCCCTACA GCTCACCTAA AACCCAGCAT AAAGCCAACC AGAGCTTCCT GCCACCGGGA 
TGGGAAATGA GGATCGCACC CAACGGACGG CCGTTCTTCA TCGACCACAA CAGCAGAACC 
ACCACATGGG AGGATCCCCG CTTGAAGTAT CCCGTTCACA TGAGGACAAA AGCGTCTCTA 
GACCCGGGCG ACCTCGGCCC ACTTCCTAAT CTTCCAGAGG AGCCTGGGTG GGAGGAACGA 
GTGCATGCTG ATGGAAGAAC CTTTTACATC GATCACAACA ACAAAAAAAC ACAGTGGGAA 
GACCCTCGTC TCCAGAGTCC TGCCATAACT GGACCCGCGG TGCCGTATTC AAGAGAATTC 
AAGCAGAAAT ACGACTACTT CAGGAAGAAG TTAAAGAAAC CGGCAGATAT CCCCAACCGC 
TTTGAGATGA AGCTGCACAG GAATAACATC TTTGAGGAGT CCTACAGACG AATCATGTCC 
CTCAAGAGAC CTGACAGCCT GAAGGCCCGT CTGTGGATTG AGTTTGAGTC AGAGAAGGGT 
CTGGATTATG GCGGTGTGGC CCGAGAATGG TTCTTCCTGC TCTCTAAGGA GATGTTCAAC 
CCTTATTATG GGCTGTTCGA GTACTCGGCC ACAGATAACT ACACCCTTCA GATCAATCCC 
AACTCTGGCC TGTGTAATGA GGATCACCTC TCATACTTCA AGTTCATTGG TCGAGTGGCC 
GGCATGGCTG TTTATCATGG CAAACTCTTG GACGGTTTCT TCATTCGACC GTTTTATAAG 
ATGATGCTGG GCAAACAGAT CACACTGAAT GACATGGAGT CAGTGGACAG CGAATACTAC 
AACTCCCTGA AGTGGATTCT GGAAAACGAT CCCACAGAAT TAGACTTACG GTTTTGTATT 
GATGAGGACA ACTTTGGACA GACATATCAG GTAGATTTGA AGCCAAGCGG ATCAGACATG 
GTTGTAACCA ACGACAACAA GAAGGAATAC ATCGATCTGG TGATTCAGTG GAGGTTTGTG 
AACAGGGTCC AGAAACAGAT GAACGCCTTT TTGGAAGGCT TCACTGAACT CATTCCCATC 
GACTTAATAA AGATTTTTGA TGAAAACGAA CTGGAGCTGT TGATGTGTGG TCTGGGAGAT 
GTGGATGTCA ATGACTGGAG GCAACACACT GTCTATAAGA ACGGCTACTG TCCAAATCAC 
CCCGTCATCC AGTGGTTCTG GAAGGCTGTG TTGTTAATGG ATGCAGAAAA GCGCATCAGG 
CTTCTACAGT TTGTCACTGG AACATCCAGA GTGCCAATGA ACGGCTTTGC TGAACTCTAC 
GGTTCCAATG GGCCGCAGTT GTTTACTATT GAGCAGTGGG GAACTCCTGA CAAACTCCCT 
CGGGCTCACA CCTGCTTTAA TCGCTTGGAC CTCCCAATGT ATGAAACGTT TGAAGACCTG 
CGAGAGAAGC TTCTGATGGC GGTGGAAAAC GCTCAAGGCT TTGAGGGCGT CGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324688.1
CDS	341..4177
Translation	MSHRLRLYFGSGRSNTAPELEELNGTVEEEGDISYNSTGAMVVHAQPRNGGHAESPEPGTVVGSDASLKRSSSMFIPQLQGQAESRTTKSTSVQISLQRTTAPHEDALPFDPPPAYPQDPAPPYTEPGESLPLPPPPTYYKSCQNVEQTQKDTEPKRQVFCVRPCDGQNGYTHGNPQGITIGGVCIRRRSADGSDVQQFRIMPYSEDGHNGHNWSVQQCSDRGPGAQRLIFQLQKDQGRQPSSTSQPDVSDLGITGSKGSRLVRYPRIRLERSTSQPLQPRGAPSRLGSMPNLHAPISEMETESDFIENGAQGCTFKIRKEQNSQQPKFKIYFSQGGEHSPILERNISIGKAPTCSNPQAHIGLDRPKNGHFNNGQTRDDFLGQVDVPLTHLPTEDPAMERPYTFKDFLLRPRSHKSRVKGYLRLKMAYLPKNGGHEEESGEMREEAEGWEVTDSTDPGSQRPQQLLPPLPPGWEEKVDNLGRTYYVNHNNRTTQWKRPSSVDVVSETENDNQLRQINQEAHRVFRSRRHISEDLENEQLDIRDIDDSWEPISEEDPTLLTDGLNHSLSGPSSLAMPLSSTPDFSDEISLRLSLAPDTNGEIPGPSSAVQSHLSSRLRSSSMTDGVSDQAQPPTPTSSQTRRTRAQTVTGVEDTMSPSASYALTTPGLPPGWEERKDPKGRTYYVNHNNRSTTWTRPILQQHTEDGASTSVAAVGGATAATPPASTPSTSSGHLSEPQVRRPRSLSSPTVTLSSPLEGANNVPIRRAVKDTFSNPQSPQPSPYSSPKTQHKANQSFLPPGWEMRIAPNGRPFFIDHNSRTTTWEDPRLKYPVHMRTKASLDPGDLGPLPNLPEEPGWEERVHADGRTFYIDHNNKKTQWEDPRLQSPAITGPAVPYSREFKQKYDYFRKKLKKPADIPNRFEMKLHRNNIFEESYRRIMSLKRPDSLKARLWIEFESEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFKFIGRVAGMAVYHGKLLDGFFIRPFYKMMLGKQITLNDMESVDSEYYNSLKWILENDPTELDLRFCIDEDNFGQTYQVDLKPSGSDMVVTNDNKKEYIDLVIQWRFVNRVQKQMNAFLEGFTELIPIDLIKIFDENELELLMCGLGDVDVNDWRQHTVYKNGYCPNHPVIQWFWKAVLLMDAEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGTPDKLPRAHTCFNRLDLPMYETFEDLREKLLMAVENAQGFEGVD

Target ORF information:

RefSeq Version	XM_021469013.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio neural precursor cell expressed, developmentally down-regulated 4-like (nedd4l), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021469013.1

ORF Insert Sequence:

ATGTCTCACC GGCTTCGGTT GTATTTTGGA TCTGGTCGCA GTAACACTGC TCCAGAGTTG 
GAAGAGCTGA ATGGGACAGT GGAGGAGGAA GGTGACATAA GCTACAACAG TACAGGAGCC 
ATGGTGGTCC ATGCACAACC ACGTAACGGA GGACATGCAG AAAGTCCAGA ACCAGGCACA 
GTTGTTGGTT CGGATGCCTC TCTAAAGCGA AGCTCTTCCA TGTTCATCCC ACAGCTCCAG 
GGTCAAGCTG AGTCACGGAC AACCAAGAGC ACCTCTGTTC AAATATCTCT TCAACGCACA 
ACTGCCCCTC ATGAAGATGC ATTACCCTTT GATCCACCTC CAGCGTACCC CCAGGATCCA 
GCTCCACCAT ACACTGAACC TGGTGAAAGT TTGCCCTTAC CTCCACCTCC AACCTACTAC 
AAATCCTGCC AAAATGTGGA ACAAACTCAA AAAGATACTG AACCTAAGAG GCAAGTCTTT 
TGTGTCCGGC CATGTGATGG TCAAAATGGT TATACCCATG GAAATCCTCA AGGGATCACT 
ATTGGAGGTG TCTGTATTAG AAGAAGATCT GCAGATGGCT CGGATGTCCA GCAGTTCAGG 
ATTATGCCAT ATAGCGAGGA TGGGCACAAT GGGCACAATT GGTCTGTCCA GCAATGCTCA 
GACAGAGGTC CTGGTGCACA AAGACTAATT TTTCAGCTCC AAAAGGACCA AGGACGGCAA 
CCAAGCTCTA CCTCTCAGCC TGACGTGAGT GATTTAGGCA TCACAGGTTC TAAAGGTTCC 
CGCTTGGTGC GGTACCCCAG GATTCGACTT GAGCGGAGCA CGTCACAGCC ATTGCAGCCT 
AGAGGAGCTC CTTCACGCCT TGGATCAATG CCAAACCTTC ATGCTCCTAT CTCAGAAATG 
GAAACGGAGA GTGATTTTAT TGAGAATGGT GCTCAAGGTT GCACTTTCAA AATTAGGAAG 
GAACAGAATT CCCAACAACC GAAGTTTAAG ATTTATTTCA GCCAGGGCGG CGAGCACAGT 
CCCATCCTCG AAAGGAATAT TAGCATTGGA AAGGCGCCTA CCTGCAGCAA TCCACAGGCT 
CACATTGGTC TTGATCGGCC CAAGAACGGT CATTTCAACA ATGGTCAGAC CAGAGATGAC 
TTCCTGGGGC AGGTGGACGT GCCTCTTACA CACTTACCGA CTGAAGACCC TGCCATGGAG 
CGTCCATACA CGTTTAAAGA CTTTCTCCTG CGTCCAAGAA GTCATAAATC TCGGGTGAAA 
GGTTACCTGA GGCTCAAGAT GGCCTACCTG CCAAAGAATG GAGGACATGA GGAGGAAAGC 
GGTGAAATGA GAGAGGAGGC TGAGGGTTGG GAAGTGACAG ACTCCACAGA TCCAGGATCT 
CAGAGACCTC AGCAGCTCCT GCCTCCTCTT CCTCCGGGCT GGGAAGAGAA AGTGGATAAC 
CTGGGACGGA CGTACTATGT GAACCACAAC AACCGCACCA CACAGTGGAA GAGGCCCAGC 
AGTGTGGATG TGGTTTCAGA AACGGAAAAT GATAATCAAT TGCGGCAGAT TAACCAGGAA 
GCCCACAGAG TTTTCCGCTC TCGACGGCAC ATTAGCGAAG ACCTGGAAAA TGAGCAGCTG 
GACATCAGGG ACATAGACGA CTCATGGGAG CCCATATCAG AGGAAGATCC GACTTTATTA 
ACAGATGGTC TGAACCATTC TCTGTCGGGA CCCTCCTCTC TGGCAATGCC CCTCTCCAGC 
ACTCCAGACT TCTCAGATGA GATCAGCCTT AGATTGTCTC TCGCTCCAGA CACCAACGGA 
GAAATCCCGG GACCCAGCTC TGCTGTGCAG AGTCATCTTT CCTCTCGGCT ACGCTCCTCT 
AGTATGACTG ATGGAGTCAG TGATCAAGCT CAACCTCCCA CACCAACGAG CTCCCAGACC 
CGACGAACAA GAGCACAAAC CGTCACAGGC GTTGAGGACA CCATGTCTCC TTCGGCCTCT 
TACGCCTTGA CCACGCCAGG CCTTCCACCA GGCTGGGAGG AACGCAAAGA CCCCAAGGGA 
CGAACGTACT ACGTTAATCA TAACAACCGT AGCACCACAT GGACAAGGCC CATTCTGCAG 
CAGCACACTG AGGATGGAGC CAGCACCTCG GTTGCAGCAG TAGGGGGCGC TACAGCAGCT 
ACACCCCCTG CTTCAACCCC TTCCACCTCC AGCGGCCATC TAAGCGAACC ACAAGTGCGC 
AGACCCCGTA GCCTCAGTTC CCCTACCGTC ACTCTCTCTT CCCCACTAGA GGGAGCCAAT 
AACGTCCCTA TACGGCGAGC CGTAAAGGAC ACATTCTCCA ACCCTCAGTC TCCACAGCCT 
TCGCCCTACA GCTCACCTAA AACCCAGCAT AAAGCCAACC AGAGCTTCCT GCCACCGGGA 
TGGGAAATGA GGATCGCACC CAACGGACGG CCGTTCTTCA TCGACCACAA CAGCAGAACC 
ACCACATGGG AGGATCCCCG CTTGAAGTAT CCCGTTCACA TGAGGACAAA AGCGTCTCTA 
GACCCGGGCG ACCTCGGCCC ACTTCCTAAT CTTCCAGAGG AGCCTGGGTG GGAGGAACGA 
GTGCATGCTG ATGGAAGAAC CTTTTACATC GATCACAACA ACAAAAAAAC ACAGTGGGAA 
GACCCTCGTC TCCAGAGTCC TGCCATAACT GGACCCGCGG TGCCGTATTC AAGAGAATTC 
AAGCAGAAAT ACGACTACTT CAGGAAGAAG TTAAAGAAAC CGGCAGATAT CCCCAACCGC 
TTTGAGATGA AGCTGCACAG GAATAACATC TTTGAGGAGT CCTACAGACG AATCATGTCC 
CTCAAGAGAC CTGACAGCCT GAAGGCCCGT CTGTGGATTG AGTTTGAGTC AGAGAAGGGT 
CTGGATTATG GCGGTGTGGC CCGAGAATGG TTCTTCCTGC TCTCTAAGGA GATGTTCAAC 
CCTTATTATG GGCTGTTCGA GTACTCGGCC ACAGATAACT ACACCCTTCA GATCAATCCC 
AACTCTGGCC TGTGTAATGA GGATCACCTC TCATACTTCA AGTTCATTGG TCGAGTGGCC 
GGCATGGCTG TTTATCATGG CAAACTCTTG GACGGTTTCT TCATTCGACC GTTTTATAAG 
ATGATGCTGG GCAAACAGAT CACACTGAAT GACATGGAGT CAGTGGACAG CGAATACTAC 
AACTCCCTGA AGTGGATTCT GGAAAACGAT CCCACAGAAT TAGACTTACG GTTTTGTATT 
GATGAGGACA ACTTTGGACA GACATATCAG GTAGATTTGA AGCCAAGCGG ATCAGACATG 
GTTGTAACCA ACGACAACAA GAAGGAATAC ATCGATCTGG TGATTCAGTG GAGGTTTGTG 
AACAGGGTCC AGAAACAGAT GAACGCCTTT TTGGAAGGCT TCACTGAACT CATTCCCATC 
GACTTAATAA AGATTTTTGA TGAAAACGAA CTGGAGCTGT TGATGTGTGG TCTGGGAGAT 
GTGGATGTCA ATGACTGGAG GCAACACACT GTCTATAAGA ACGGCTACTG TCCAAATCAC 
CCCGTCATCC AGTGGTTCTG GAAGGCTGTG TTGTTAATGG ATGCAGAAAA GCGCATCAGG 
CTTCTACAGT TTGTCACTGG AACATCCAGA GTGCCAATGA ACGGCTTTGC TGAACTCTAC 
GGTTCCAATG GGCCGCAGTT GTTTACTATT GAGCAGTGGG GAACTCCTGA CAAACTCCCT 
CGGGCTCACA CCTGCTTTAA TCGCTTGGAC CTCCCAATGT ATGAAACGTT TGAAGACCTG 
CGAGAGAAGC TTCTGATGGC GGTGGAAAAC GCTCAAGGCT TTGAGGGCGT CGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nedd4l ( XM_021469014.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324689.1 Danio rerio neural precursor cell expressed, developmentally down-regulated 4-like (nedd4l), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa151013
Clone ID Related Accession (Same CDS sequence)	XM_021469014.1
Accession Version	XM_021469014.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3837bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase NEDD4-like isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCACC GGCTTCGGTT GTATTTTGGA TCTGGTCGCA GTAACACTGC TCCAGAGTTG 
GAAGAGCTGA ATGGGACAGT GGAGGAGGAA GGTGACATAA GCTACAACAG TACAGGAGCC 
ATGGTGGTCC ATGCACAACC ACGTAACGGA GGACATGCAG AAAGTCCAGA ACCAGGCACA 
GTTGTTGGTT CGGATGCCTC TCTAAAGCGA AGCTCTTCCA TGTTCATCCC ACAGCTCCAG 
GGTCAAGCTG AGTCACGGAC AACCAAGAGC ACCTCTGTTC AAATATCTCT TCAACGCACA 
ACTGCCCCTC ATGAAGATGC ATTACCCTTT GATCCACCTC CAGCGTACCC CCAGGATCCA 
GCTCCACCAT ACACTGAACC TGGTGAAAGT TTGCCCTTAC CTCCACCTCC AACCTACTAC 
AAATCCTGCC AAAATGTGGA ACAAACTCAA AAAGATACTG AACCTAAGAG GCAAGTCTTT 
TGTGTCCGGC CATGTGATGG TCAAAATGGT TATACCCATG GAAATCCTCA AGGGATCACT 
ATTGGAGGTG TCTGTATTAG AAGAAGATCT GCAGATGGCT CGGATGTCCA GCAGTTCAGG 
ATTATGCCAT ATAGCGAGGA TGGGCACAAT GGGCACAATT GGTCTGTCCA GCAATGCTCA 
GACAGAGGTC CTGGTGCACA AAGACTAATT TTTCAGCTCC AAAAGGACCA AGGACGGCAA 
CCAAGCTCTA CCTCTCAGCC TGACGTGAGT GATTTAGGCA TCACAGGTTC TAAAGGTTCC 
CGCTTGGTGC GGTACCCCAG GATTCGACTT GAGCGGAGCA CGTCACAGCC ATTGCAGCCT 
AGAGGAGCTC CTTCACGCCT TGGATCAATG CCAAACCTTC ATGCTCCTAT CTCAGAAATG 
GAAACGGAGA GTGATTTTAT TGAGAATGGT GCTCAAGGTT GCACTTTCAA AATTAGGAAG 
GAACAGAATT CCCAACAACC GAAGTTTAAG ATTTATTTCA GCCAGGGCGG CGAGCACAGT 
CCCATCCTCG AAAGGAATAT TAGCATTGGA AAGGCGCCTA CCTGCAGCAA TCCACAGGCT 
CACATTGGTC TTGATCGGCC CAAGAACGGT CATTTCAACA ATGGTCAGAC CAGAGATGAC 
TTCCTGGGGC AGGTGGACGT GCCTCTTACA CACTTACCGA CTGAAGACCC TGCCATGGAG 
CGTCCATACA CGTTTAAAGA CTTTCTCCTG CGTCCAAGAA GTCATAAATC TCGGGTGAAA 
GGTTACCTGA GGCTCAAGAT GGCCTACCTG CCAAAGAATG GAGGACATGA GGAGGAAAGC 
GGTGAAATGA GAGAGGAGGC TGAGGGTTGG GAAGTGACAG ACTCCACAGA TCCAGGATCT 
CAGAGACCTC AGCAGCTCCT GCCTCCTCTT CCTCCGGGCT GGGAAGAGAA AGTGGATAAC 
CTGGGACGGA CGTACTATGT GAACCACAAC AACCGCACCA CACAGTGGAA GAGGCCCAGC 
AGTGTGGATG TGGTTTCAGA AACGGAAAAT GATAATCAAT TGCGGCAGAT TAACCAGGAA 
GCCCACAGAG TTTTCCGCTC TCGACGGCAC ATTAGCGAAG ACCTGGAAAA TGAGCAGCTG 
GACATCAGGG ACATAGACGA CTCATGGGAG CCCATATCAG AGGAAGATCC GACTTTATTA 
ACAGATGGTC TGAACCATTC TCTGTCGGGA CCCTCCTCTC TGGCAATGCC CCTCTCCAGC 
ACTCCAGACT TCTCAGATGA GATCAGCCTT AGATTGTCTC TCGCTCCAGA CACCAACGGA 
GAAATCCCGG GACCCAGCTC TGCTGTGCAG AGTCATCTTT CCTCTCGGCT ACGCTCCTCT 
AGTATGACTG ATGGAGTCAG TGATCAAGCT CAACCTCCCA CACCAACGCA GAGCTCCCAG 
ACCCGACGAA CAAGAGCACA AACCGTCACA GGCGTTGAGG ACACCATGTC TCCTTCGGCC 
TCTTACGCCT TGACCACGCC AGGCCTTCCA CCAGGCTGGG AGGAACGCAA AGACCCCAAG 
GGACGAACGT ACTACGTTAA TCATAACAAC CGTAGCACCA CATGGACAAG GCCCATTCTG 
CAGCACACTG AGGATGGAGC CAGCACCTCG GTTGCAGCAG TAGGGGGCGC TACAGCAGCT 
ACACCCCCTG CTTCAACCCC TTCCACCTCC AGCGGCCATC TAAGCGAACC ACAAGTGCGC 
AGACCCCGTA GCCTCAGTTC CCCTACCGTC ACTCTCTCTT CCCCACTAGA GGGAGCCAAT 
AACGTCCCTA TACGGCGAGC CGTAAAGGAC ACATTCTCCA ACCCTCAGTC TCCACAGCCT 
TCGCCCTACA GCTCACCTAA AACCCAGCAT AAAGCCAACC AGAGCTTCCT GCCACCGGGA 
TGGGAAATGA GGATCGCACC CAACGGACGG CCGTTCTTCA TCGACCACAA CAGCAGAACC 
ACCACATGGG AGGATCCCCG CTTGAAGTAT CCCGTTCACA TGAGGACAAA AGCGTCTCTA 
GACCCGGGCG ACCTCGGCCC ACTTCCTAAT CTTCCAGAGG AGCCTGGGTG GGAGGAACGA 
GTGCATGCTG ATGGAAGAAC CTTTTACATC GATCACAACA ACAAAAAAAC ACAGTGGGAA 
GACCCTCGTC TCCAGAGTCC TGCCATAACT GGACCCGCGG TGCCGTATTC AAGAGAATTC 
AAGCAGAAAT ACGACTACTT CAGGAAGAAG TTAAAGAAAC CGGCAGATAT CCCCAACCGC 
TTTGAGATGA AGCTGCACAG GAATAACATC TTTGAGGAGT CCTACAGACG AATCATGTCC 
CTCAAGAGAC CTGACAGCCT GAAGGCCCGT CTGTGGATTG AGTTTGAGTC AGAGAAGGGT 
CTGGATTATG GCGGTGTGGC CCGAGAATGG TTCTTCCTGC TCTCTAAGGA GATGTTCAAC 
CCTTATTATG GGCTGTTCGA GTACTCGGCC ACAGATAACT ACACCCTTCA GATCAATCCC 
AACTCTGGCC TGTGTAATGA GGATCACCTC TCATACTTCA AGTTCATTGG TCGAGTGGCC 
GGCATGGCTG TTTATCATGG CAAACTCTTG GACGGTTTCT TCATTCGACC GTTTTATAAG 
ATGATGCTGG GCAAACAGAT CACACTGAAT GACATGGAGT CAGTGGACAG CGAATACTAC 
AACTCCCTGA AGTGGATTCT GGAAAACGAT CCCACAGAAT TAGACTTACG GTTTTGTATT 
GATGAGGACA ACTTTGGACA GACATATCAG GTAGATTTGA AGCCAAGCGG ATCAGACATG 
GTTGTAACCA ACGACAACAA GAAGGAATAC ATCGATCTGG TGATTCAGTG GAGGTTTGTG 
AACAGGGTCC AGAAACAGAT GAACGCCTTT TTGGAAGGCT TCACTGAACT CATTCCCATC 
GACTTAATAA AGATTTTTGA TGAAAACGAA CTGGAGCTGT TGATGTGTGG TCTGGGAGAT 
GTGGATGTCA ATGACTGGAG GCAACACACT GTCTATAAGA ACGGCTACTG TCCAAATCAC 
CCCGTCATCC AGTGGTTCTG GAAGGCTGTG TTGTTAATGG ATGCAGAAAA GCGCATCAGG 
CTTCTACAGT TTGTCACTGG AACATCCAGA GTGCCAATGA ACGGCTTTGC TGAACTCTAC 
GGTTCCAATG GGCCGCAGTT GTTTACTATT GAGCAGTGGG GAACTCCTGA CAAACTCCCT 
CGGGCTCACA CCTGCTTTAA TCGCTTGGAC CTCCCAATGT ATGAAACGTT TGAAGACCTG 
CGAGAGAAGC TTCTGATGGC GGTGGAAAAC GCTCAAGGCT TTGAGGGCGT CGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324689.1
CDS	340..4176
Translation	MSHRLRLYFGSGRSNTAPELEELNGTVEEEGDISYNSTGAMVVHAQPRNGGHAESPEPGTVVGSDASLKRSSSMFIPQLQGQAESRTTKSTSVQISLQRTTAPHEDALPFDPPPAYPQDPAPPYTEPGESLPLPPPPTYYKSCQNVEQTQKDTEPKRQVFCVRPCDGQNGYTHGNPQGITIGGVCIRRRSADGSDVQQFRIMPYSEDGHNGHNWSVQQCSDRGPGAQRLIFQLQKDQGRQPSSTSQPDVSDLGITGSKGSRLVRYPRIRLERSTSQPLQPRGAPSRLGSMPNLHAPISEMETESDFIENGAQGCTFKIRKEQNSQQPKFKIYFSQGGEHSPILERNISIGKAPTCSNPQAHIGLDRPKNGHFNNGQTRDDFLGQVDVPLTHLPTEDPAMERPYTFKDFLLRPRSHKSRVKGYLRLKMAYLPKNGGHEEESGEMREEAEGWEVTDSTDPGSQRPQQLLPPLPPGWEEKVDNLGRTYYVNHNNRTTQWKRPSSVDVVSETENDNQLRQINQEAHRVFRSRRHISEDLENEQLDIRDIDDSWEPISEEDPTLLTDGLNHSLSGPSSLAMPLSSTPDFSDEISLRLSLAPDTNGEIPGPSSAVQSHLSSRLRSSSMTDGVSDQAQPPTPTQSSQTRRTRAQTVTGVEDTMSPSASYALTTPGLPPGWEERKDPKGRTYYVNHNNRSTTWTRPILQHTEDGASTSVAAVGGATAATPPASTPSTSSGHLSEPQVRRPRSLSSPTVTLSSPLEGANNVPIRRAVKDTFSNPQSPQPSPYSSPKTQHKANQSFLPPGWEMRIAPNGRPFFIDHNSRTTTWEDPRLKYPVHMRTKASLDPGDLGPLPNLPEEPGWEERVHADGRTFYIDHNNKKTQWEDPRLQSPAITGPAVPYSREFKQKYDYFRKKLKKPADIPNRFEMKLHRNNIFEESYRRIMSLKRPDSLKARLWIEFESEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFKFIGRVAGMAVYHGKLLDGFFIRPFYKMMLGKQITLNDMESVDSEYYNSLKWILENDPTELDLRFCIDEDNFGQTYQVDLKPSGSDMVVTNDNKKEYIDLVIQWRFVNRVQKQMNAFLEGFTELIPIDLIKIFDENELELLMCGLGDVDVNDWRQHTVYKNGYCPNHPVIQWFWKAVLLMDAEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGTPDKLPRAHTCFNRLDLPMYETFEDLREKLLMAVENAQGFEGVD

Target ORF information:

RefSeq Version	XM_021469014.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio neural precursor cell expressed, developmentally down-regulated 4-like (nedd4l), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021469014.1

ORF Insert Sequence:

ATGTCTCACC GGCTTCGGTT GTATTTTGGA TCTGGTCGCA GTAACACTGC TCCAGAGTTG 
GAAGAGCTGA ATGGGACAGT GGAGGAGGAA GGTGACATAA GCTACAACAG TACAGGAGCC 
ATGGTGGTCC ATGCACAACC ACGTAACGGA GGACATGCAG AAAGTCCAGA ACCAGGCACA 
GTTGTTGGTT CGGATGCCTC TCTAAAGCGA AGCTCTTCCA TGTTCATCCC ACAGCTCCAG 
GGTCAAGCTG AGTCACGGAC AACCAAGAGC ACCTCTGTTC AAATATCTCT TCAACGCACA 
ACTGCCCCTC ATGAAGATGC ATTACCCTTT GATCCACCTC CAGCGTACCC CCAGGATCCA 
GCTCCACCAT ACACTGAACC TGGTGAAAGT TTGCCCTTAC CTCCACCTCC AACCTACTAC 
AAATCCTGCC AAAATGTGGA ACAAACTCAA AAAGATACTG AACCTAAGAG GCAAGTCTTT 
TGTGTCCGGC CATGTGATGG TCAAAATGGT TATACCCATG GAAATCCTCA AGGGATCACT 
ATTGGAGGTG TCTGTATTAG AAGAAGATCT GCAGATGGCT CGGATGTCCA GCAGTTCAGG 
ATTATGCCAT ATAGCGAGGA TGGGCACAAT GGGCACAATT GGTCTGTCCA GCAATGCTCA 
GACAGAGGTC CTGGTGCACA AAGACTAATT TTTCAGCTCC AAAAGGACCA AGGACGGCAA 
CCAAGCTCTA CCTCTCAGCC TGACGTGAGT GATTTAGGCA TCACAGGTTC TAAAGGTTCC 
CGCTTGGTGC GGTACCCCAG GATTCGACTT GAGCGGAGCA CGTCACAGCC ATTGCAGCCT 
AGAGGAGCTC CTTCACGCCT TGGATCAATG CCAAACCTTC ATGCTCCTAT CTCAGAAATG 
GAAACGGAGA GTGATTTTAT TGAGAATGGT GCTCAAGGTT GCACTTTCAA AATTAGGAAG 
GAACAGAATT CCCAACAACC GAAGTTTAAG ATTTATTTCA GCCAGGGCGG CGAGCACAGT 
CCCATCCTCG AAAGGAATAT TAGCATTGGA AAGGCGCCTA CCTGCAGCAA TCCACAGGCT 
CACATTGGTC TTGATCGGCC CAAGAACGGT CATTTCAACA ATGGTCAGAC CAGAGATGAC 
TTCCTGGGGC AGGTGGACGT GCCTCTTACA CACTTACCGA CTGAAGACCC TGCCATGGAG 
CGTCCATACA CGTTTAAAGA CTTTCTCCTG CGTCCAAGAA GTCATAAATC TCGGGTGAAA 
GGTTACCTGA GGCTCAAGAT GGCCTACCTG CCAAAGAATG GAGGACATGA GGAGGAAAGC 
GGTGAAATGA GAGAGGAGGC TGAGGGTTGG GAAGTGACAG ACTCCACAGA TCCAGGATCT 
CAGAGACCTC AGCAGCTCCT GCCTCCTCTT CCTCCGGGCT GGGAAGAGAA AGTGGATAAC 
CTGGGACGGA CGTACTATGT GAACCACAAC AACCGCACCA CACAGTGGAA GAGGCCCAGC 
AGTGTGGATG TGGTTTCAGA AACGGAAAAT GATAATCAAT TGCGGCAGAT TAACCAGGAA 
GCCCACAGAG TTTTCCGCTC TCGACGGCAC ATTAGCGAAG ACCTGGAAAA TGAGCAGCTG 
GACATCAGGG ACATAGACGA CTCATGGGAG CCCATATCAG AGGAAGATCC GACTTTATTA 
ACAGATGGTC TGAACCATTC TCTGTCGGGA CCCTCCTCTC TGGCAATGCC CCTCTCCAGC 
ACTCCAGACT TCTCAGATGA GATCAGCCTT AGATTGTCTC TCGCTCCAGA CACCAACGGA 
GAAATCCCGG GACCCAGCTC TGCTGTGCAG AGTCATCTTT CCTCTCGGCT ACGCTCCTCT 
AGTATGACTG ATGGAGTCAG TGATCAAGCT CAACCTCCCA CACCAACGCA GAGCTCCCAG 
ACCCGACGAA CAAGAGCACA AACCGTCACA GGCGTTGAGG ACACCATGTC TCCTTCGGCC 
TCTTACGCCT TGACCACGCC AGGCCTTCCA CCAGGCTGGG AGGAACGCAA AGACCCCAAG 
GGACGAACGT ACTACGTTAA TCATAACAAC CGTAGCACCA CATGGACAAG GCCCATTCTG 
CAGCACACTG AGGATGGAGC CAGCACCTCG GTTGCAGCAG TAGGGGGCGC TACAGCAGCT 
ACACCCCCTG CTTCAACCCC TTCCACCTCC AGCGGCCATC TAAGCGAACC ACAAGTGCGC 
AGACCCCGTA GCCTCAGTTC CCCTACCGTC ACTCTCTCTT CCCCACTAGA GGGAGCCAAT 
AACGTCCCTA TACGGCGAGC CGTAAAGGAC ACATTCTCCA ACCCTCAGTC TCCACAGCCT 
TCGCCCTACA GCTCACCTAA AACCCAGCAT AAAGCCAACC AGAGCTTCCT GCCACCGGGA 
TGGGAAATGA GGATCGCACC CAACGGACGG CCGTTCTTCA TCGACCACAA CAGCAGAACC 
ACCACATGGG AGGATCCCCG CTTGAAGTAT CCCGTTCACA TGAGGACAAA AGCGTCTCTA 
GACCCGGGCG ACCTCGGCCC ACTTCCTAAT CTTCCAGAGG AGCCTGGGTG GGAGGAACGA 
GTGCATGCTG ATGGAAGAAC CTTTTACATC GATCACAACA ACAAAAAAAC ACAGTGGGAA 
GACCCTCGTC TCCAGAGTCC TGCCATAACT GGACCCGCGG TGCCGTATTC AAGAGAATTC 
AAGCAGAAAT ACGACTACTT CAGGAAGAAG TTAAAGAAAC CGGCAGATAT CCCCAACCGC 
TTTGAGATGA AGCTGCACAG GAATAACATC TTTGAGGAGT CCTACAGACG AATCATGTCC 
CTCAAGAGAC CTGACAGCCT GAAGGCCCGT CTGTGGATTG AGTTTGAGTC AGAGAAGGGT 
CTGGATTATG GCGGTGTGGC CCGAGAATGG TTCTTCCTGC TCTCTAAGGA GATGTTCAAC 
CCTTATTATG GGCTGTTCGA GTACTCGGCC ACAGATAACT ACACCCTTCA GATCAATCCC 
AACTCTGGCC TGTGTAATGA GGATCACCTC TCATACTTCA AGTTCATTGG TCGAGTGGCC 
GGCATGGCTG TTTATCATGG CAAACTCTTG GACGGTTTCT TCATTCGACC GTTTTATAAG 
ATGATGCTGG GCAAACAGAT CACACTGAAT GACATGGAGT CAGTGGACAG CGAATACTAC 
AACTCCCTGA AGTGGATTCT GGAAAACGAT CCCACAGAAT TAGACTTACG GTTTTGTATT 
GATGAGGACA ACTTTGGACA GACATATCAG GTAGATTTGA AGCCAAGCGG ATCAGACATG 
GTTGTAACCA ACGACAACAA GAAGGAATAC ATCGATCTGG TGATTCAGTG GAGGTTTGTG 
AACAGGGTCC AGAAACAGAT GAACGCCTTT TTGGAAGGCT TCACTGAACT CATTCCCATC 
GACTTAATAA AGATTTTTGA TGAAAACGAA CTGGAGCTGT TGATGTGTGG TCTGGGAGAT 
GTGGATGTCA ATGACTGGAG GCAACACACT GTCTATAAGA ACGGCTACTG TCCAAATCAC 
CCCGTCATCC AGTGGTTCTG GAAGGCTGTG TTGTTAATGG ATGCAGAAAA GCGCATCAGG 
CTTCTACAGT TTGTCACTGG AACATCCAGA GTGCCAATGA ACGGCTTTGC TGAACTCTAC 
GGTTCCAATG GGCCGCAGTT GTTTACTATT GAGCAGTGGG GAACTCCTGA CAAACTCCCT 
CGGGCTCACA CCTGCTTTAA TCGCTTGGAC CTCCCAATGT ATGAAACGTT TGAAGACCTG 
CGAGAGAAGC TTCTGATGGC GGTGGAAAAC GCTCAAGGCT TTGAGGGCGT CGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nedd4l ( XM_021469015.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324690.1 Danio rerio neural precursor cell expressed, developmentally down-regulated 4-like (nedd4l), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa151014
Clone ID Related Accession (Same CDS sequence)	XM_021469015.1
Accession Version	XM_021469015.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3837bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase NEDD4-like isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCACC GGCTTCGGTT GTATTTTGGA TCTGGTCGCA GTAACACTGC TCCAGAGTTG 
GAAGAGCTGA ATGGGACAGT GGAGGAGGAA GGTGACATAA GCTACAACAG TACAGGAGCC 
ATGGTGGTCC ATGCACAACC ACGTAACGGA GGACATGCAG AAAGTCCAGA ACCAGGCACA 
GTTGTTGGTT CGGATGCCTC TCTAAAGCGA AGCTCTTCCA TGTTCATCCC ACAGCTCCAG 
GGTCAAGCTG AGTCACGGAC AACCAAGAGC ACCTCTGTTC AAATATCTCT TCAACGCACA 
ACTGCCCCTC ATGAAGATGC ATTACCCTTT GATCCACCTC CAGCGTACCC CCAGGATCCA 
GCTCCACCAT ACACTGAACC TGGTGAAAGT TTGCCCTTAC CTCCACCTCC AACCTACTAC 
AAATCCTGCC AAAATGTGGA ACAAACTCAA AAAGATACTG AACCTAAGAG GCAAGTCTTT 
TGTGTCCGGC CATGTGATGG TCAAAATGGT TATACCCATG GAAATCCTCA AGGGATCACT 
ATTGGAGGTG TCTGTATTAG AAGAAGATCT GCAGATGGCT CGGATGTCCA GCAGTTCAGG 
ATTATGCCAT ATAGCGAGGA TGGGCACAAT GGGCACAATT GGTCTGTCCA GCAATGCTCA 
GACAGAGGTC CTGGTGCACA AAGACTAATT TTTCAGCTCC AAAAGGACCA AGGACGGCAA 
CCAAGCTCTA CCTCTCAGCC TGACGTGAGT GATTTAGGCA TCACAGGTTC TAAAGGTTCC 
CGCTTGGTGC GGTACCCCAG GATTCGACTT GAGCGGAGCA CGTCACAGCC ATTGCAGCCT 
AGAGGAGCTC CTTCACGCCT TGGATCAATG CCAAACCTTC ATGCTCCTAT CTCAGAAATG 
GAAACGGAGA GTGATTTTAT TGAGAATGGT GCTCAAGGTT GCACTTTCAA AATTAGGAAG 
GAACAGAATT CCCAACAACC GAAGTTTAAG ATTTATTTCA GCCAGGGCGG CGAGCACAGT 
CCCATCCTCG AAAGGAATAT TAGCATTGGA AAGGCGCCTA CCTGCAGCAA TCCACAGGCT 
CACATTGGTC TTGATCGGCC CAAGAACGGT CATTTCAACA ATGGTCAGAC CAGAGATGAC 
TTCCTGGGGC AGGTGGACGT GCCTCTTACA CACTTACCGA CTGAAGACCC TGCCATGGAG 
CGTCCATACA CGTTTAAAGA CTTTCTCCTG CGTCCAAGAA GTCATAAATC TCGGGTGAAA 
GGTTACCTGA GGCTCAAGAT GGCCTACCTG CCAAAGAATG GAGGACATGA GGAGGAAAGC 
GGTGAAATGA GAGAGGAGGC TGAGGGTTGG GAAGTGACAG ACTCCACAGA TCCAGGATCT 
CAGAGACCTC AGCAGCTCCT GCCTCCTCTT CCTCCGGGCT GGGAAGAGAA AGTGGATAAC 
CTGGGACGGA CGTACTATGT GAACCACAAC AACCGCACCA CACAGTGGAA GAGGCCCAGC 
AGTGTGGATG TGGTTTCAGA AACGGAAAAT GATAATCAAT TGCGGCAGAT TAACCAGGAA 
GCCCACAGAG TTTTCCGCTC TCGACGGCAC ATTAGCGAAG ACCTGGAAAA TGAGCAGCTG 
GACATCAGGG ACATAGACGA CTCATGGGAG CCCATATCAG AGGAAGATCC GACTTTATTA 
ACAGATGGTC TGAACCATTC TCTGTCGGGA CCCTCCTCTC TGGCAATGCC CCTCTCCAGC 
ACTCCAGACT TCTCAGATGA GATCAGCCTT AGATTGTCTC TCGCTCCAGA CACCAACGGA 
GAAATCCCGG GACCCAGCTC TGCTGTGAGT CATCTTTCCT CTCGGCTACG CTCCTCTAGT 
ATGACTGATG GAGTCAGTGA TCAAGCTCAA CCTCCCACAC CAACGCAGAG CTCCCAGACC 
CGACGAACAA GAGCACAAAC CGTCACAGGC GTTGAGGACA CCATGTCTCC TTCGGCCTCT 
TACGCCTTGA CCACGCCAGG CCTTCCACCA GGCTGGGAGG AACGCAAAGA CCCCAAGGGA 
CGAACGTACT ACGTTAATCA TAACAACCGT AGCACCACAT GGACAAGGCC CATTCTGCAG 
CAGCACACTG AGGATGGAGC CAGCACCTCG GTTGCAGCAG TAGGGGGCGC TACAGCAGCT 
ACACCCCCTG CTTCAACCCC TTCCACCTCC AGCGGCCATC TAAGCGAACC ACAAGTGCGC 
AGACCCCGTA GCCTCAGTTC CCCTACCGTC ACTCTCTCTT CCCCACTAGA GGGAGCCAAT 
AACGTCCCTA TACGGCGAGC CGTAAAGGAC ACATTCTCCA ACCCTCAGTC TCCACAGCCT 
TCGCCCTACA GCTCACCTAA AACCCAGCAT AAAGCCAACC AGAGCTTCCT GCCACCGGGA 
TGGGAAATGA GGATCGCACC CAACGGACGG CCGTTCTTCA TCGACCACAA CAGCAGAACC 
ACCACATGGG AGGATCCCCG CTTGAAGTAT CCCGTTCACA TGAGGACAAA AGCGTCTCTA 
GACCCGGGCG ACCTCGGCCC ACTTCCTAAT CTTCCAGAGG AGCCTGGGTG GGAGGAACGA 
GTGCATGCTG ATGGAAGAAC CTTTTACATC GATCACAACA ACAAAAAAAC ACAGTGGGAA 
GACCCTCGTC TCCAGAGTCC TGCCATAACT GGACCCGCGG TGCCGTATTC AAGAGAATTC 
AAGCAGAAAT ACGACTACTT CAGGAAGAAG TTAAAGAAAC CGGCAGATAT CCCCAACCGC 
TTTGAGATGA AGCTGCACAG GAATAACATC TTTGAGGAGT CCTACAGACG AATCATGTCC 
CTCAAGAGAC CTGACAGCCT GAAGGCCCGT CTGTGGATTG AGTTTGAGTC AGAGAAGGGT 
CTGGATTATG GCGGTGTGGC CCGAGAATGG TTCTTCCTGC TCTCTAAGGA GATGTTCAAC 
CCTTATTATG GGCTGTTCGA GTACTCGGCC ACAGATAACT ACACCCTTCA GATCAATCCC 
AACTCTGGCC TGTGTAATGA GGATCACCTC TCATACTTCA AGTTCATTGG TCGAGTGGCC 
GGCATGGCTG TTTATCATGG CAAACTCTTG GACGGTTTCT TCATTCGACC GTTTTATAAG 
ATGATGCTGG GCAAACAGAT CACACTGAAT GACATGGAGT CAGTGGACAG CGAATACTAC 
AACTCCCTGA AGTGGATTCT GGAAAACGAT CCCACAGAAT TAGACTTACG GTTTTGTATT 
GATGAGGACA ACTTTGGACA GACATATCAG GTAGATTTGA AGCCAAGCGG ATCAGACATG 
GTTGTAACCA ACGACAACAA GAAGGAATAC ATCGATCTGG TGATTCAGTG GAGGTTTGTG 
AACAGGGTCC AGAAACAGAT GAACGCCTTT TTGGAAGGCT TCACTGAACT CATTCCCATC 
GACTTAATAA AGATTTTTGA TGAAAACGAA CTGGAGCTGT TGATGTGTGG TCTGGGAGAT 
GTGGATGTCA ATGACTGGAG GCAACACACT GTCTATAAGA ACGGCTACTG TCCAAATCAC 
CCCGTCATCC AGTGGTTCTG GAAGGCTGTG TTGTTAATGG ATGCAGAAAA GCGCATCAGG 
CTTCTACAGT TTGTCACTGG AACATCCAGA GTGCCAATGA ACGGCTTTGC TGAACTCTAC 
GGTTCCAATG GGCCGCAGTT GTTTACTATT GAGCAGTGGG GAACTCCTGA CAAACTCCCT 
CGGGCTCACA CCTGCTTTAA TCGCTTGGAC CTCCCAATGT ATGAAACGTT TGAAGACCTG 
CGAGAGAAGC TTCTGATGGC GGTGGAAAAC GCTCAAGGCT TTGAGGGCGT CGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324690.1
CDS	345..4181
Translation	MSHRLRLYFGSGRSNTAPELEELNGTVEEEGDISYNSTGAMVVHAQPRNGGHAESPEPGTVVGSDASLKRSSSMFIPQLQGQAESRTTKSTSVQISLQRTTAPHEDALPFDPPPAYPQDPAPPYTEPGESLPLPPPPTYYKSCQNVEQTQKDTEPKRQVFCVRPCDGQNGYTHGNPQGITIGGVCIRRRSADGSDVQQFRIMPYSEDGHNGHNWSVQQCSDRGPGAQRLIFQLQKDQGRQPSSTSQPDVSDLGITGSKGSRLVRYPRIRLERSTSQPLQPRGAPSRLGSMPNLHAPISEMETESDFIENGAQGCTFKIRKEQNSQQPKFKIYFSQGGEHSPILERNISIGKAPTCSNPQAHIGLDRPKNGHFNNGQTRDDFLGQVDVPLTHLPTEDPAMERPYTFKDFLLRPRSHKSRVKGYLRLKMAYLPKNGGHEEESGEMREEAEGWEVTDSTDPGSQRPQQLLPPLPPGWEEKVDNLGRTYYVNHNNRTTQWKRPSSVDVVSETENDNQLRQINQEAHRVFRSRRHISEDLENEQLDIRDIDDSWEPISEEDPTLLTDGLNHSLSGPSSLAMPLSSTPDFSDEISLRLSLAPDTNGEIPGPSSAVSHLSSRLRSSSMTDGVSDQAQPPTPTQSSQTRRTRAQTVTGVEDTMSPSASYALTTPGLPPGWEERKDPKGRTYYVNHNNRSTTWTRPILQQHTEDGASTSVAAVGGATAATPPASTPSTSSGHLSEPQVRRPRSLSSPTVTLSSPLEGANNVPIRRAVKDTFSNPQSPQPSPYSSPKTQHKANQSFLPPGWEMRIAPNGRPFFIDHNSRTTTWEDPRLKYPVHMRTKASLDPGDLGPLPNLPEEPGWEERVHADGRTFYIDHNNKKTQWEDPRLQSPAITGPAVPYSREFKQKYDYFRKKLKKPADIPNRFEMKLHRNNIFEESYRRIMSLKRPDSLKARLWIEFESEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFKFIGRVAGMAVYHGKLLDGFFIRPFYKMMLGKQITLNDMESVDSEYYNSLKWILENDPTELDLRFCIDEDNFGQTYQVDLKPSGSDMVVTNDNKKEYIDLVIQWRFVNRVQKQMNAFLEGFTELIPIDLIKIFDENELELLMCGLGDVDVNDWRQHTVYKNGYCPNHPVIQWFWKAVLLMDAEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGTPDKLPRAHTCFNRLDLPMYETFEDLREKLLMAVENAQGFEGVD

Target ORF information:

RefSeq Version	XM_021469015.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio neural precursor cell expressed, developmentally down-regulated 4-like (nedd4l), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021469015.1

ORF Insert Sequence:

ATGTCTCACC GGCTTCGGTT GTATTTTGGA TCTGGTCGCA GTAACACTGC TCCAGAGTTG 
GAAGAGCTGA ATGGGACAGT GGAGGAGGAA GGTGACATAA GCTACAACAG TACAGGAGCC 
ATGGTGGTCC ATGCACAACC ACGTAACGGA GGACATGCAG AAAGTCCAGA ACCAGGCACA 
GTTGTTGGTT CGGATGCCTC TCTAAAGCGA AGCTCTTCCA TGTTCATCCC ACAGCTCCAG 
GGTCAAGCTG AGTCACGGAC AACCAAGAGC ACCTCTGTTC AAATATCTCT TCAACGCACA 
ACTGCCCCTC ATGAAGATGC ATTACCCTTT GATCCACCTC CAGCGTACCC CCAGGATCCA 
GCTCCACCAT ACACTGAACC TGGTGAAAGT TTGCCCTTAC CTCCACCTCC AACCTACTAC 
AAATCCTGCC AAAATGTGGA ACAAACTCAA AAAGATACTG AACCTAAGAG GCAAGTCTTT 
TGTGTCCGGC CATGTGATGG TCAAAATGGT TATACCCATG GAAATCCTCA AGGGATCACT 
ATTGGAGGTG TCTGTATTAG AAGAAGATCT GCAGATGGCT CGGATGTCCA GCAGTTCAGG 
ATTATGCCAT ATAGCGAGGA TGGGCACAAT GGGCACAATT GGTCTGTCCA GCAATGCTCA 
GACAGAGGTC CTGGTGCACA AAGACTAATT TTTCAGCTCC AAAAGGACCA AGGACGGCAA 
CCAAGCTCTA CCTCTCAGCC TGACGTGAGT GATTTAGGCA TCACAGGTTC TAAAGGTTCC 
CGCTTGGTGC GGTACCCCAG GATTCGACTT GAGCGGAGCA CGTCACAGCC ATTGCAGCCT 
AGAGGAGCTC CTTCACGCCT TGGATCAATG CCAAACCTTC ATGCTCCTAT CTCAGAAATG 
GAAACGGAGA GTGATTTTAT TGAGAATGGT GCTCAAGGTT GCACTTTCAA AATTAGGAAG 
GAACAGAATT CCCAACAACC GAAGTTTAAG ATTTATTTCA GCCAGGGCGG CGAGCACAGT 
CCCATCCTCG AAAGGAATAT TAGCATTGGA AAGGCGCCTA CCTGCAGCAA TCCACAGGCT 
CACATTGGTC TTGATCGGCC CAAGAACGGT CATTTCAACA ATGGTCAGAC CAGAGATGAC 
TTCCTGGGGC AGGTGGACGT GCCTCTTACA CACTTACCGA CTGAAGACCC TGCCATGGAG 
CGTCCATACA CGTTTAAAGA CTTTCTCCTG CGTCCAAGAA GTCATAAATC TCGGGTGAAA 
GGTTACCTGA GGCTCAAGAT GGCCTACCTG CCAAAGAATG GAGGACATGA GGAGGAAAGC 
GGTGAAATGA GAGAGGAGGC TGAGGGTTGG GAAGTGACAG ACTCCACAGA TCCAGGATCT 
CAGAGACCTC AGCAGCTCCT GCCTCCTCTT CCTCCGGGCT GGGAAGAGAA AGTGGATAAC 
CTGGGACGGA CGTACTATGT GAACCACAAC AACCGCACCA CACAGTGGAA GAGGCCCAGC 
AGTGTGGATG TGGTTTCAGA AACGGAAAAT GATAATCAAT TGCGGCAGAT TAACCAGGAA 
GCCCACAGAG TTTTCCGCTC TCGACGGCAC ATTAGCGAAG ACCTGGAAAA TGAGCAGCTG 
GACATCAGGG ACATAGACGA CTCATGGGAG CCCATATCAG AGGAAGATCC GACTTTATTA 
ACAGATGGTC TGAACCATTC TCTGTCGGGA CCCTCCTCTC TGGCAATGCC CCTCTCCAGC 
ACTCCAGACT TCTCAGATGA GATCAGCCTT AGATTGTCTC TCGCTCCAGA CACCAACGGA 
GAAATCCCGG GACCCAGCTC TGCTGTGAGT CATCTTTCCT CTCGGCTACG CTCCTCTAGT 
ATGACTGATG GAGTCAGTGA TCAAGCTCAA CCTCCCACAC CAACGCAGAG CTCCCAGACC 
CGACGAACAA GAGCACAAAC CGTCACAGGC GTTGAGGACA CCATGTCTCC TTCGGCCTCT 
TACGCCTTGA CCACGCCAGG CCTTCCACCA GGCTGGGAGG AACGCAAAGA CCCCAAGGGA 
CGAACGTACT ACGTTAATCA TAACAACCGT AGCACCACAT GGACAAGGCC CATTCTGCAG 
CAGCACACTG AGGATGGAGC CAGCACCTCG GTTGCAGCAG TAGGGGGCGC TACAGCAGCT 
ACACCCCCTG CTTCAACCCC TTCCACCTCC AGCGGCCATC TAAGCGAACC ACAAGTGCGC 
AGACCCCGTA GCCTCAGTTC CCCTACCGTC ACTCTCTCTT CCCCACTAGA GGGAGCCAAT 
AACGTCCCTA TACGGCGAGC CGTAAAGGAC ACATTCTCCA ACCCTCAGTC TCCACAGCCT 
TCGCCCTACA GCTCACCTAA AACCCAGCAT AAAGCCAACC AGAGCTTCCT GCCACCGGGA 
TGGGAAATGA GGATCGCACC CAACGGACGG CCGTTCTTCA TCGACCACAA CAGCAGAACC 
ACCACATGGG AGGATCCCCG CTTGAAGTAT CCCGTTCACA TGAGGACAAA AGCGTCTCTA 
GACCCGGGCG ACCTCGGCCC ACTTCCTAAT CTTCCAGAGG AGCCTGGGTG GGAGGAACGA 
GTGCATGCTG ATGGAAGAAC CTTTTACATC GATCACAACA ACAAAAAAAC ACAGTGGGAA 
GACCCTCGTC TCCAGAGTCC TGCCATAACT GGACCCGCGG TGCCGTATTC AAGAGAATTC 
AAGCAGAAAT ACGACTACTT CAGGAAGAAG TTAAAGAAAC CGGCAGATAT CCCCAACCGC 
TTTGAGATGA AGCTGCACAG GAATAACATC TTTGAGGAGT CCTACAGACG AATCATGTCC 
CTCAAGAGAC CTGACAGCCT GAAGGCCCGT CTGTGGATTG AGTTTGAGTC AGAGAAGGGT 
CTGGATTATG GCGGTGTGGC CCGAGAATGG TTCTTCCTGC TCTCTAAGGA GATGTTCAAC 
CCTTATTATG GGCTGTTCGA GTACTCGGCC ACAGATAACT ACACCCTTCA GATCAATCCC 
AACTCTGGCC TGTGTAATGA GGATCACCTC TCATACTTCA AGTTCATTGG TCGAGTGGCC 
GGCATGGCTG TTTATCATGG CAAACTCTTG GACGGTTTCT TCATTCGACC GTTTTATAAG 
ATGATGCTGG GCAAACAGAT CACACTGAAT GACATGGAGT CAGTGGACAG CGAATACTAC 
AACTCCCTGA AGTGGATTCT GGAAAACGAT CCCACAGAAT TAGACTTACG GTTTTGTATT 
GATGAGGACA ACTTTGGACA GACATATCAG GTAGATTTGA AGCCAAGCGG ATCAGACATG 
GTTGTAACCA ACGACAACAA GAAGGAATAC ATCGATCTGG TGATTCAGTG GAGGTTTGTG 
AACAGGGTCC AGAAACAGAT GAACGCCTTT TTGGAAGGCT TCACTGAACT CATTCCCATC 
GACTTAATAA AGATTTTTGA TGAAAACGAA CTGGAGCTGT TGATGTGTGG TCTGGGAGAT 
GTGGATGTCA ATGACTGGAG GCAACACACT GTCTATAAGA ACGGCTACTG TCCAAATCAC 
CCCGTCATCC AGTGGTTCTG GAAGGCTGTG TTGTTAATGG ATGCAGAAAA GCGCATCAGG 
CTTCTACAGT TTGTCACTGG AACATCCAGA GTGCCAATGA ACGGCTTTGC TGAACTCTAC 
GGTTCCAATG GGCCGCAGTT GTTTACTATT GAGCAGTGGG GAACTCCTGA CAAACTCCCT 
CGGGCTCACA CCTGCTTTAA TCGCTTGGAC CTCCCAATGT ATGAAACGTT TGAAGACCTG 
CGAGAGAAGC TTCTGATGGC GGTGGAAAAC GCTCAAGGCT TTGAGGGCGT CGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nedd4l ( XM_021469016.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324691.1 Danio rerio neural precursor cell expressed, developmentally down-regulated 4-like (nedd4l), transcript variant X5, mRNA.

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CloneID	ODa151015
Clone ID Related Accession (Same CDS sequence)	XM_021469016.1
Accession Version	XM_021469016.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3834bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase NEDD4-like isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCACC GGCTTCGGTT GTATTTTGGA TCTGGTCGCA GTAACACTGC TCCAGAGTTG 
GAAGAGCTGA ATGGGACAGT GGAGGAGGAA GGTGACATAA GCTACAACAG TACAGGAGCC 
ATGGTGGTCC ATGCACAACC ACGTAACGGA GGACATGCAG AAAGTCCAGA ACCAGGCACA 
GTTGTTGGTT CGGATGCCTC TCTAAAGCGA AGCTCTTCCA TGTTCATCCC ACAGCTCCAG 
GGTCAAGCTG AGTCACGGAC AACCAAGAGC ACCTCTGTTC AAATATCTCT TCAACGCACA 
ACTGCCCCTC ATGAAGATGC ATTACCCTTT GATCCACCTC CAGCGTACCC CCAGGATCCA 
GCTCCACCAT ACACTGAACC TGGTGAAAGT TTGCCCTTAC CTCCACCTCC AACCTACTAC 
AAATCCTGCC AAAATGTGGA ACAAACTCAA AAAGATACTG AACCTAAGAG GCAAGTCTTT 
TGTGTCCGGC CATGTGATGG TCAAAATGGT TATACCCATG GAAATCCTCA AGGGATCACT 
ATTGGAGGTG TCTGTATTAG AAGAAGATCT GCAGATGGCT CGGATGTCCA GCAGTTCAGG 
ATTATGCCAT ATAGCGAGGA TGGGCACAAT GGGCACAATT GGTCTGTCCA GCAATGCTCA 
GACAGAGGTC CTGGTGCACA AAGACTAATT TTTCAGCTCC AAAAGGACCA AGGACGGCAA 
CCAAGCTCTA CCTCTCAGCC TGACGTGAGT GATTTAGGCA TCACAGGTTC TAAAGGTTCC 
CGCTTGGTGC GGTACCCCAG GATTCGACTT GAGCGGAGCA CGTCACAGCC ATTGCAGCCT 
AGAGGAGCTC CTTCACGCCT TGGATCAATG CCAAACCTTC ATGCTCCTAT CTCAGAAATG 
GAAACGGAGA GTGATTTTAT TGAGAATGGT GCTCAAGGTT GCACTTTCAA AATTAGGAAG 
GAACAGAATT CCCAACAACC GAAGTTTAAG ATTTATTTCA GCCAGGGCGG CGAGCACAGT 
CCCATCCTCG AAAGGAATAT TAGCATTGGA AAGGCGCCTA CCTGCAGCAA TCCACAGGCT 
CACATTGGTC TTGATCGGCC CAAGAACGGT CATTTCAACA ATGGTCAGAC CAGAGATGAC 
TTCCTGGGGC AGGTGGACGT GCCTCTTACA CACTTACCGA CTGAAGACCC TGCCATGGAG 
CGTCCATACA CGTTTAAAGA CTTTCTCCTG CGTCCAAGAA GTCATAAATC TCGGGTGAAA 
GGTTACCTGA GGCTCAAGAT GGCCTACCTG CCAAAGAATG GAGGACATGA GGAGGAAAGC 
GGTGAAATGA GAGAGGAGGC TGAGGGTTGG GAAGTGACAG ACTCCACAGA TCCAGGATCT 
CAGAGACCTC AGCAGCTCCT GCCTCCTCTT CCTCCGGGCT GGGAAGAGAA AGTGGATAAC 
CTGGGACGGA CGTACTATGT GAACCACAAC AACCGCACCA CACAGTGGAA GAGGCCCAGC 
AGTGTGGATG TGGTTTCAGA AACGGAAAAT GATAATCAAT TGCGGCAGAT TAACCAGGAA 
GCCCACAGAG TTTTCCGCTC TCGACGGCAC ATTAGCGAAG ACCTGGAAAA TGAGCAGCTG 
GACATCAGGG ACATAGACGA CTCATGGGAG CCCATATCAG AGGAAGATCC GACTTTATTA 
ACAGATGGTC TGAACCATTC TCTGTCGGGA CCCTCCTCTC TGGCAATGCC CCTCTCCAGC 
ACTCCAGACT TCTCAGATGA GATCAGCCTT AGATTGTCTC TCGCTCCAGA CACCAACGGA 
GAAATCCCGG GACCCAGCTC TGCTGTGCAG AGTCATCTTT CCTCTCGGCT ACGCTCCTCT 
AGTATGACTG ATGGAGTCAG TGATCAAGCT CAACCTCCCA CACCAACGAG CTCCCAGACC 
CGACGAACAA GAGCACAAAC CGTCACAGGC GTTGAGGACA CCATGTCTCC TTCGGCCTCT 
TACGCCTTGA CCACGCCAGG CCTTCCACCA GGCTGGGAGG AACGCAAAGA CCCCAAGGGA 
CGAACGTACT ACGTTAATCA TAACAACCGT AGCACCACAT GGACAAGGCC CATTCTGCAG 
CACACTGAGG ATGGAGCCAG CACCTCGGTT GCAGCAGTAG GGGGCGCTAC AGCAGCTACA 
CCCCCTGCTT CAACCCCTTC CACCTCCAGC GGCCATCTAA GCGAACCACA AGTGCGCAGA 
CCCCGTAGCC TCAGTTCCCC TACCGTCACT CTCTCTTCCC CACTAGAGGG AGCCAATAAC 
GTCCCTATAC GGCGAGCCGT AAAGGACACA TTCTCCAACC CTCAGTCTCC ACAGCCTTCG 
CCCTACAGCT CACCTAAAAC CCAGCATAAA GCCAACCAGA GCTTCCTGCC ACCGGGATGG 
GAAATGAGGA TCGCACCCAA CGGACGGCCG TTCTTCATCG ACCACAACAG CAGAACCACC 
ACATGGGAGG ATCCCCGCTT GAAGTATCCC GTTCACATGA GGACAAAAGC GTCTCTAGAC 
CCGGGCGACC TCGGCCCACT TCCTAATCTT CCAGAGGAGC CTGGGTGGGA GGAACGAGTG 
CATGCTGATG GAAGAACCTT TTACATCGAT CACAACAACA AAAAAACACA GTGGGAAGAC 
CCTCGTCTCC AGAGTCCTGC CATAACTGGA CCCGCGGTGC CGTATTCAAG AGAATTCAAG 
CAGAAATACG ACTACTTCAG GAAGAAGTTA AAGAAACCGG CAGATATCCC CAACCGCTTT 
GAGATGAAGC TGCACAGGAA TAACATCTTT GAGGAGTCCT ACAGACGAAT CATGTCCCTC 
AAGAGACCTG ACAGCCTGAA GGCCCGTCTG TGGATTGAGT TTGAGTCAGA GAAGGGTCTG 
GATTATGGCG GTGTGGCCCG AGAATGGTTC TTCCTGCTCT CTAAGGAGAT GTTCAACCCT 
TATTATGGGC TGTTCGAGTA CTCGGCCACA GATAACTACA CCCTTCAGAT CAATCCCAAC 
TCTGGCCTGT GTAATGAGGA TCACCTCTCA TACTTCAAGT TCATTGGTCG AGTGGCCGGC 
ATGGCTGTTT ATCATGGCAA ACTCTTGGAC GGTTTCTTCA TTCGACCGTT TTATAAGATG 
ATGCTGGGCA AACAGATCAC ACTGAATGAC ATGGAGTCAG TGGACAGCGA ATACTACAAC 
TCCCTGAAGT GGATTCTGGA AAACGATCCC ACAGAATTAG ACTTACGGTT TTGTATTGAT 
GAGGACAACT TTGGACAGAC ATATCAGGTA GATTTGAAGC CAAGCGGATC AGACATGGTT 
GTAACCAACG ACAACAAGAA GGAATACATC GATCTGGTGA TTCAGTGGAG GTTTGTGAAC 
AGGGTCCAGA AACAGATGAA CGCCTTTTTG GAAGGCTTCA CTGAACTCAT TCCCATCGAC 
TTAATAAAGA TTTTTGATGA AAACGAACTG GAGCTGTTGA TGTGTGGTCT GGGAGATGTG 
GATGTCAATG ACTGGAGGCA ACACACTGTC TATAAGAACG GCTACTGTCC AAATCACCCC 
GTCATCCAGT GGTTCTGGAA GGCTGTGTTG TTAATGGATG CAGAAAAGCG CATCAGGCTT 
CTACAGTTTG TCACTGGAAC ATCCAGAGTG CCAATGAACG GCTTTGCTGA ACTCTACGGT 
TCCAATGGGC CGCAGTTGTT TACTATTGAG CAGTGGGGAA CTCCTGACAA ACTCCCTCGG 
GCTCACACCT GCTTTAATCG CTTGGACCTC CCAATGTATG AAACGTTTGA AGACCTGCGA 
GAGAAGCTTC TGATGGCGGT GGAAAACGCT CAAGGCTTTG AGGGCGTCGA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324691.1
CDS	339..4172
Translation	MSHRLRLYFGSGRSNTAPELEELNGTVEEEGDISYNSTGAMVVHAQPRNGGHAESPEPGTVVGSDASLKRSSSMFIPQLQGQAESRTTKSTSVQISLQRTTAPHEDALPFDPPPAYPQDPAPPYTEPGESLPLPPPPTYYKSCQNVEQTQKDTEPKRQVFCVRPCDGQNGYTHGNPQGITIGGVCIRRRSADGSDVQQFRIMPYSEDGHNGHNWSVQQCSDRGPGAQRLIFQLQKDQGRQPSSTSQPDVSDLGITGSKGSRLVRYPRIRLERSTSQPLQPRGAPSRLGSMPNLHAPISEMETESDFIENGAQGCTFKIRKEQNSQQPKFKIYFSQGGEHSPILERNISIGKAPTCSNPQAHIGLDRPKNGHFNNGQTRDDFLGQVDVPLTHLPTEDPAMERPYTFKDFLLRPRSHKSRVKGYLRLKMAYLPKNGGHEEESGEMREEAEGWEVTDSTDPGSQRPQQLLPPLPPGWEEKVDNLGRTYYVNHNNRTTQWKRPSSVDVVSETENDNQLRQINQEAHRVFRSRRHISEDLENEQLDIRDIDDSWEPISEEDPTLLTDGLNHSLSGPSSLAMPLSSTPDFSDEISLRLSLAPDTNGEIPGPSSAVQSHLSSRLRSSSMTDGVSDQAQPPTPTSSQTRRTRAQTVTGVEDTMSPSASYALTTPGLPPGWEERKDPKGRTYYVNHNNRSTTWTRPILQHTEDGASTSVAAVGGATAATPPASTPSTSSGHLSEPQVRRPRSLSSPTVTLSSPLEGANNVPIRRAVKDTFSNPQSPQPSPYSSPKTQHKANQSFLPPGWEMRIAPNGRPFFIDHNSRTTTWEDPRLKYPVHMRTKASLDPGDLGPLPNLPEEPGWEERVHADGRTFYIDHNNKKTQWEDPRLQSPAITGPAVPYSREFKQKYDYFRKKLKKPADIPNRFEMKLHRNNIFEESYRRIMSLKRPDSLKARLWIEFESEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFKFIGRVAGMAVYHGKLLDGFFIRPFYKMMLGKQITLNDMESVDSEYYNSLKWILENDPTELDLRFCIDEDNFGQTYQVDLKPSGSDMVVTNDNKKEYIDLVIQWRFVNRVQKQMNAFLEGFTELIPIDLIKIFDENELELLMCGLGDVDVNDWRQHTVYKNGYCPNHPVIQWFWKAVLLMDAEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGTPDKLPRAHTCFNRLDLPMYETFEDLREKLLMAVENAQGFEGVD

Target ORF information:

RefSeq Version	XM_021469016.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio neural precursor cell expressed, developmentally down-regulated 4-like (nedd4l), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021469016.1

ORF Insert Sequence:

ATGTCTCACC GGCTTCGGTT GTATTTTGGA TCTGGTCGCA GTAACACTGC TCCAGAGTTG 
GAAGAGCTGA ATGGGACAGT GGAGGAGGAA GGTGACATAA GCTACAACAG TACAGGAGCC 
ATGGTGGTCC ATGCACAACC ACGTAACGGA GGACATGCAG AAAGTCCAGA ACCAGGCACA 
GTTGTTGGTT CGGATGCCTC TCTAAAGCGA AGCTCTTCCA TGTTCATCCC ACAGCTCCAG 
GGTCAAGCTG AGTCACGGAC AACCAAGAGC ACCTCTGTTC AAATATCTCT TCAACGCACA 
ACTGCCCCTC ATGAAGATGC ATTACCCTTT GATCCACCTC CAGCGTACCC CCAGGATCCA 
GCTCCACCAT ACACTGAACC TGGTGAAAGT TTGCCCTTAC CTCCACCTCC AACCTACTAC 
AAATCCTGCC AAAATGTGGA ACAAACTCAA AAAGATACTG AACCTAAGAG GCAAGTCTTT 
TGTGTCCGGC CATGTGATGG TCAAAATGGT TATACCCATG GAAATCCTCA AGGGATCACT 
ATTGGAGGTG TCTGTATTAG AAGAAGATCT GCAGATGGCT CGGATGTCCA GCAGTTCAGG 
ATTATGCCAT ATAGCGAGGA TGGGCACAAT GGGCACAATT GGTCTGTCCA GCAATGCTCA 
GACAGAGGTC CTGGTGCACA AAGACTAATT TTTCAGCTCC AAAAGGACCA AGGACGGCAA 
CCAAGCTCTA CCTCTCAGCC TGACGTGAGT GATTTAGGCA TCACAGGTTC TAAAGGTTCC 
CGCTTGGTGC GGTACCCCAG GATTCGACTT GAGCGGAGCA CGTCACAGCC ATTGCAGCCT 
AGAGGAGCTC CTTCACGCCT TGGATCAATG CCAAACCTTC ATGCTCCTAT CTCAGAAATG 
GAAACGGAGA GTGATTTTAT TGAGAATGGT GCTCAAGGTT GCACTTTCAA AATTAGGAAG 
GAACAGAATT CCCAACAACC GAAGTTTAAG ATTTATTTCA GCCAGGGCGG CGAGCACAGT 
CCCATCCTCG AAAGGAATAT TAGCATTGGA AAGGCGCCTA CCTGCAGCAA TCCACAGGCT 
CACATTGGTC TTGATCGGCC CAAGAACGGT CATTTCAACA ATGGTCAGAC CAGAGATGAC 
TTCCTGGGGC AGGTGGACGT GCCTCTTACA CACTTACCGA CTGAAGACCC TGCCATGGAG 
CGTCCATACA CGTTTAAAGA CTTTCTCCTG CGTCCAAGAA GTCATAAATC TCGGGTGAAA 
GGTTACCTGA GGCTCAAGAT GGCCTACCTG CCAAAGAATG GAGGACATGA GGAGGAAAGC 
GGTGAAATGA GAGAGGAGGC TGAGGGTTGG GAAGTGACAG ACTCCACAGA TCCAGGATCT 
CAGAGACCTC AGCAGCTCCT GCCTCCTCTT CCTCCGGGCT GGGAAGAGAA AGTGGATAAC 
CTGGGACGGA CGTACTATGT GAACCACAAC AACCGCACCA CACAGTGGAA GAGGCCCAGC 
AGTGTGGATG TGGTTTCAGA AACGGAAAAT GATAATCAAT TGCGGCAGAT TAACCAGGAA 
GCCCACAGAG TTTTCCGCTC TCGACGGCAC ATTAGCGAAG ACCTGGAAAA TGAGCAGCTG 
GACATCAGGG ACATAGACGA CTCATGGGAG CCCATATCAG AGGAAGATCC GACTTTATTA 
ACAGATGGTC TGAACCATTC TCTGTCGGGA CCCTCCTCTC TGGCAATGCC CCTCTCCAGC 
ACTCCAGACT TCTCAGATGA GATCAGCCTT AGATTGTCTC TCGCTCCAGA CACCAACGGA 
GAAATCCCGG GACCCAGCTC TGCTGTGCAG AGTCATCTTT CCTCTCGGCT ACGCTCCTCT 
AGTATGACTG ATGGAGTCAG TGATCAAGCT CAACCTCCCA CACCAACGAG CTCCCAGACC 
CGACGAACAA GAGCACAAAC CGTCACAGGC GTTGAGGACA CCATGTCTCC TTCGGCCTCT 
TACGCCTTGA CCACGCCAGG CCTTCCACCA GGCTGGGAGG AACGCAAAGA CCCCAAGGGA 
CGAACGTACT ACGTTAATCA TAACAACCGT AGCACCACAT GGACAAGGCC CATTCTGCAG 
CACACTGAGG ATGGAGCCAG CACCTCGGTT GCAGCAGTAG GGGGCGCTAC AGCAGCTACA 
CCCCCTGCTT CAACCCCTTC CACCTCCAGC GGCCATCTAA GCGAACCACA AGTGCGCAGA 
CCCCGTAGCC TCAGTTCCCC TACCGTCACT CTCTCTTCCC CACTAGAGGG AGCCAATAAC 
GTCCCTATAC GGCGAGCCGT AAAGGACACA TTCTCCAACC CTCAGTCTCC ACAGCCTTCG 
CCCTACAGCT CACCTAAAAC CCAGCATAAA GCCAACCAGA GCTTCCTGCC ACCGGGATGG 
GAAATGAGGA TCGCACCCAA CGGACGGCCG TTCTTCATCG ACCACAACAG CAGAACCACC 
ACATGGGAGG ATCCCCGCTT GAAGTATCCC GTTCACATGA GGACAAAAGC GTCTCTAGAC 
CCGGGCGACC TCGGCCCACT TCCTAATCTT CCAGAGGAGC CTGGGTGGGA GGAACGAGTG 
CATGCTGATG GAAGAACCTT TTACATCGAT CACAACAACA AAAAAACACA GTGGGAAGAC 
CCTCGTCTCC AGAGTCCTGC CATAACTGGA CCCGCGGTGC CGTATTCAAG AGAATTCAAG 
CAGAAATACG ACTACTTCAG GAAGAAGTTA AAGAAACCGG CAGATATCCC CAACCGCTTT 
GAGATGAAGC TGCACAGGAA TAACATCTTT GAGGAGTCCT ACAGACGAAT CATGTCCCTC 
AAGAGACCTG ACAGCCTGAA GGCCCGTCTG TGGATTGAGT TTGAGTCAGA GAAGGGTCTG 
GATTATGGCG GTGTGGCCCG AGAATGGTTC TTCCTGCTCT CTAAGGAGAT GTTCAACCCT 
TATTATGGGC TGTTCGAGTA CTCGGCCACA GATAACTACA CCCTTCAGAT CAATCCCAAC 
TCTGGCCTGT GTAATGAGGA TCACCTCTCA TACTTCAAGT TCATTGGTCG AGTGGCCGGC 
ATGGCTGTTT ATCATGGCAA ACTCTTGGAC GGTTTCTTCA TTCGACCGTT TTATAAGATG 
ATGCTGGGCA AACAGATCAC ACTGAATGAC ATGGAGTCAG TGGACAGCGA ATACTACAAC 
TCCCTGAAGT GGATTCTGGA AAACGATCCC ACAGAATTAG ACTTACGGTT TTGTATTGAT 
GAGGACAACT TTGGACAGAC ATATCAGGTA GATTTGAAGC CAAGCGGATC AGACATGGTT 
GTAACCAACG ACAACAAGAA GGAATACATC GATCTGGTGA TTCAGTGGAG GTTTGTGAAC 
AGGGTCCAGA AACAGATGAA CGCCTTTTTG GAAGGCTTCA CTGAACTCAT TCCCATCGAC 
TTAATAAAGA TTTTTGATGA AAACGAACTG GAGCTGTTGA TGTGTGGTCT GGGAGATGTG 
GATGTCAATG ACTGGAGGCA ACACACTGTC TATAAGAACG GCTACTGTCC AAATCACCCC 
GTCATCCAGT GGTTCTGGAA GGCTGTGTTG TTAATGGATG CAGAAAAGCG CATCAGGCTT 
CTACAGTTTG TCACTGGAAC ATCCAGAGTG CCAATGAACG GCTTTGCTGA ACTCTACGGT 
TCCAATGGGC CGCAGTTGTT TACTATTGAG CAGTGGGGAA CTCCTGACAA ACTCCCTCGG 
GCTCACACCT GCTTTAATCG CTTGGACCTC CCAATGTATG AAACGTTTGA AGACCTGCGA 
GAGAAGCTTC TGATGGCGGT GGAAAACGCT CAAGGCTTTG AGGGCGTCGA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nedd4l ( XM_021469017.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324692.1 Danio rerio neural precursor cell expressed, developmentally down-regulated 4-like (nedd4l), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ODa151016
Clone ID Related Accession (Same CDS sequence)	XM_021469017.1
Accession Version	XM_021469017.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3834bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase NEDD4-like isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCACC GGCTTCGGTT GTATTTTGGA TCTGGTCGCA GTAACACTGC TCCAGAGTTG 
GAAGAGCTGA ATGGGACAGT GGAGGAGGAA GGTGACATAA GCTACAACAG TACAGGAGCC 
ATGGTGGTCC ATGCACAACC ACGTAACGGA GGACATGCAG AAAGTCCAGA ACCAGGCACA 
GTTGTTGGTT CGGATGCCTC TCTAAAGCGA AGCTCTTCCA TGTTCATCCC ACAGCTCCAG 
GGTCAAGCTG AGTCACGGAC AACCAAGAGC ACCTCTGTTC AAATATCTCT TCAACGCACA 
ACTGCCCCTC ATGAAGATGC ATTACCCTTT GATCCACCTC CAGCGTACCC CCAGGATCCA 
GCTCCACCAT ACACTGAACC TGGTGAAAGT TTGCCCTTAC CTCCACCTCC AACCTACTAC 
AAATCCTGCC AAAATGTGGA ACAAACTCAA AAAGATACTG AACCTAAGAG GCAAGTCTTT 
TGTGTCCGGC CATGTGATGG TCAAAATGGT TATACCCATG GAAATCCTCA AGGGATCACT 
ATTGGAGGTG TCTGTATTAG AAGAAGATCT GCAGATGGCT CGGATGTCCA GCAGTTCAGG 
ATTATGCCAT ATAGCGAGGA TGGGCACAAT GGGCACAATT GGTCTGTCCA GCAATGCTCA 
GACAGAGGTC CTGGTGCACA AAGACTAATT TTTCAGCTCC AAAAGGACCA AGGACGGCAA 
CCAAGCTCTA CCTCTCAGCC TGACGTGAGT GATTTAGGCA TCACAGGTTC TAAAGGTTCC 
CGCTTGGTGC GGTACCCCAG GATTCGACTT GAGCGGAGCA CGTCACAGCC ATTGCAGCCT 
AGAGGAGCTC CTTCACGCCT TGGATCAATG CCAAACCTTC ATGCTCCTAT CTCAGAAATG 
GAAACGGAGA GTGATTTTAT TGAGAATGGT GCTCAAGGTT GCACTTTCAA AATTAGGAAG 
GAACAGAATT CCCAACAACC GAAGTTTAAG ATTTATTTCA GCCAGGGCGG CGAGCACAGT 
CCCATCCTCG AAAGGAATAT TAGCATTGGA AAGGCGCCTA CCTGCAGCAA TCCACAGGCT 
CACATTGGTC TTGATCGGCC CAAGAACGGT CATTTCAACA ATGGTCAGAC CAGAGATGAC 
TTCCTGGGGC AGGTGGACGT GCCTCTTACA CACTTACCGA CTGAAGACCC TGCCATGGAG 
CGTCCATACA CGTTTAAAGA CTTTCTCCTG CGTCCAAGAA GTCATAAATC TCGGGTGAAA 
GGTTACCTGA GGCTCAAGAT GGCCTACCTG CCAAAGAATG GAGGACATGA GGAGGAAAGC 
GGTGAAATGA GAGAGGAGGC TGAGGGTTGG GAAGTGACAG ACTCCACAGA TCCAGGATCT 
CAGAGACCTC AGCAGCTCCT GCCTCCTCTT CCTCCGGGCT GGGAAGAGAA AGTGGATAAC 
CTGGGACGGA CGTACTATGT GAACCACAAC AACCGCACCA CACAGTGGAA GAGGCCCAGC 
AGTGTGGATG TGGTTTCAGA AACGGAAAAT GATAATCAAT TGCGGCAGAT TAACCAGGAA 
GCCCACAGAG TTTTCCGCTC TCGACGGCAC ATTAGCGAAG ACCTGGAAAA TGAGCAGCTG 
GACATCAGGG ACATAGACGA CTCATGGGAG CCCATATCAG AGGAAGATCC GACTTTATTA 
ACAGATGGTC TGAACCATTC TCTGTCGGGA CCCTCCTCTC TGGCAATGCC CCTCTCCAGC 
ACTCCAGACT TCTCAGATGA GATCAGCCTT AGATTGTCTC TCGCTCCAGA CACCAACGGA 
GAAATCCCGG GACCCAGCTC TGCTGTGAGT CATCTTTCCT CTCGGCTACG CTCCTCTAGT 
ATGACTGATG GAGTCAGTGA TCAAGCTCAA CCTCCCACAC CAACGAGCTC CCAGACCCGA 
CGAACAAGAG CACAAACCGT CACAGGCGTT GAGGACACCA TGTCTCCTTC GGCCTCTTAC 
GCCTTGACCA CGCCAGGCCT TCCACCAGGC TGGGAGGAAC GCAAAGACCC CAAGGGACGA 
ACGTACTACG TTAATCATAA CAACCGTAGC ACCACATGGA CAAGGCCCAT TCTGCAGCAG 
CACACTGAGG ATGGAGCCAG CACCTCGGTT GCAGCAGTAG GGGGCGCTAC AGCAGCTACA 
CCCCCTGCTT CAACCCCTTC CACCTCCAGC GGCCATCTAA GCGAACCACA AGTGCGCAGA 
CCCCGTAGCC TCAGTTCCCC TACCGTCACT CTCTCTTCCC CACTAGAGGG AGCCAATAAC 
GTCCCTATAC GGCGAGCCGT AAAGGACACA TTCTCCAACC CTCAGTCTCC ACAGCCTTCG 
CCCTACAGCT CACCTAAAAC CCAGCATAAA GCCAACCAGA GCTTCCTGCC ACCGGGATGG 
GAAATGAGGA TCGCACCCAA CGGACGGCCG TTCTTCATCG ACCACAACAG CAGAACCACC 
ACATGGGAGG ATCCCCGCTT GAAGTATCCC GTTCACATGA GGACAAAAGC GTCTCTAGAC 
CCGGGCGACC TCGGCCCACT TCCTAATCTT CCAGAGGAGC CTGGGTGGGA GGAACGAGTG 
CATGCTGATG GAAGAACCTT TTACATCGAT CACAACAACA AAAAAACACA GTGGGAAGAC 
CCTCGTCTCC AGAGTCCTGC CATAACTGGA CCCGCGGTGC CGTATTCAAG AGAATTCAAG 
CAGAAATACG ACTACTTCAG GAAGAAGTTA AAGAAACCGG CAGATATCCC CAACCGCTTT 
GAGATGAAGC TGCACAGGAA TAACATCTTT GAGGAGTCCT ACAGACGAAT CATGTCCCTC 
AAGAGACCTG ACAGCCTGAA GGCCCGTCTG TGGATTGAGT TTGAGTCAGA GAAGGGTCTG 
GATTATGGCG GTGTGGCCCG AGAATGGTTC TTCCTGCTCT CTAAGGAGAT GTTCAACCCT 
TATTATGGGC TGTTCGAGTA CTCGGCCACA GATAACTACA CCCTTCAGAT CAATCCCAAC 
TCTGGCCTGT GTAATGAGGA TCACCTCTCA TACTTCAAGT TCATTGGTCG AGTGGCCGGC 
ATGGCTGTTT ATCATGGCAA ACTCTTGGAC GGTTTCTTCA TTCGACCGTT TTATAAGATG 
ATGCTGGGCA AACAGATCAC ACTGAATGAC ATGGAGTCAG TGGACAGCGA ATACTACAAC 
TCCCTGAAGT GGATTCTGGA AAACGATCCC ACAGAATTAG ACTTACGGTT TTGTATTGAT 
GAGGACAACT TTGGACAGAC ATATCAGGTA GATTTGAAGC CAAGCGGATC AGACATGGTT 
GTAACCAACG ACAACAAGAA GGAATACATC GATCTGGTGA TTCAGTGGAG GTTTGTGAAC 
AGGGTCCAGA AACAGATGAA CGCCTTTTTG GAAGGCTTCA CTGAACTCAT TCCCATCGAC 
TTAATAAAGA TTTTTGATGA AAACGAACTG GAGCTGTTGA TGTGTGGTCT GGGAGATGTG 
GATGTCAATG ACTGGAGGCA ACACACTGTC TATAAGAACG GCTACTGTCC AAATCACCCC 
GTCATCCAGT GGTTCTGGAA GGCTGTGTTG TTAATGGATG CAGAAAAGCG CATCAGGCTT 
CTACAGTTTG TCACTGGAAC ATCCAGAGTG CCAATGAACG GCTTTGCTGA ACTCTACGGT 
TCCAATGGGC CGCAGTTGTT TACTATTGAG CAGTGGGGAA CTCCTGACAA ACTCCCTCGG 
GCTCACACCT GCTTTAATCG CTTGGACCTC CCAATGTATG AAACGTTTGA AGACCTGCGA 
GAGAAGCTTC TGATGGCGGT GGAAAACGCT CAAGGCTTTG AGGGCGTCGA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324692.1
CDS	343..4176
Translation	MSHRLRLYFGSGRSNTAPELEELNGTVEEEGDISYNSTGAMVVHAQPRNGGHAESPEPGTVVGSDASLKRSSSMFIPQLQGQAESRTTKSTSVQISLQRTTAPHEDALPFDPPPAYPQDPAPPYTEPGESLPLPPPPTYYKSCQNVEQTQKDTEPKRQVFCVRPCDGQNGYTHGNPQGITIGGVCIRRRSADGSDVQQFRIMPYSEDGHNGHNWSVQQCSDRGPGAQRLIFQLQKDQGRQPSSTSQPDVSDLGITGSKGSRLVRYPRIRLERSTSQPLQPRGAPSRLGSMPNLHAPISEMETESDFIENGAQGCTFKIRKEQNSQQPKFKIYFSQGGEHSPILERNISIGKAPTCSNPQAHIGLDRPKNGHFNNGQTRDDFLGQVDVPLTHLPTEDPAMERPYTFKDFLLRPRSHKSRVKGYLRLKMAYLPKNGGHEEESGEMREEAEGWEVTDSTDPGSQRPQQLLPPLPPGWEEKVDNLGRTYYVNHNNRTTQWKRPSSVDVVSETENDNQLRQINQEAHRVFRSRRHISEDLENEQLDIRDIDDSWEPISEEDPTLLTDGLNHSLSGPSSLAMPLSSTPDFSDEISLRLSLAPDTNGEIPGPSSAVSHLSSRLRSSSMTDGVSDQAQPPTPTSSQTRRTRAQTVTGVEDTMSPSASYALTTPGLPPGWEERKDPKGRTYYVNHNNRSTTWTRPILQQHTEDGASTSVAAVGGATAATPPASTPSTSSGHLSEPQVRRPRSLSSPTVTLSSPLEGANNVPIRRAVKDTFSNPQSPQPSPYSSPKTQHKANQSFLPPGWEMRIAPNGRPFFIDHNSRTTTWEDPRLKYPVHMRTKASLDPGDLGPLPNLPEEPGWEERVHADGRTFYIDHNNKKTQWEDPRLQSPAITGPAVPYSREFKQKYDYFRKKLKKPADIPNRFEMKLHRNNIFEESYRRIMSLKRPDSLKARLWIEFESEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFKFIGRVAGMAVYHGKLLDGFFIRPFYKMMLGKQITLNDMESVDSEYYNSLKWILENDPTELDLRFCIDEDNFGQTYQVDLKPSGSDMVVTNDNKKEYIDLVIQWRFVNRVQKQMNAFLEGFTELIPIDLIKIFDENELELLMCGLGDVDVNDWRQHTVYKNGYCPNHPVIQWFWKAVLLMDAEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGTPDKLPRAHTCFNRLDLPMYETFEDLREKLLMAVENAQGFEGVD

Target ORF information:

RefSeq Version	XM_021469017.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio neural precursor cell expressed, developmentally down-regulated 4-like (nedd4l), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021469017.1

ORF Insert Sequence:

ATGTCTCACC GGCTTCGGTT GTATTTTGGA TCTGGTCGCA GTAACACTGC TCCAGAGTTG 
GAAGAGCTGA ATGGGACAGT GGAGGAGGAA GGTGACATAA GCTACAACAG TACAGGAGCC 
ATGGTGGTCC ATGCACAACC ACGTAACGGA GGACATGCAG AAAGTCCAGA ACCAGGCACA 
GTTGTTGGTT CGGATGCCTC TCTAAAGCGA AGCTCTTCCA TGTTCATCCC ACAGCTCCAG 
GGTCAAGCTG AGTCACGGAC AACCAAGAGC ACCTCTGTTC AAATATCTCT TCAACGCACA 
ACTGCCCCTC ATGAAGATGC ATTACCCTTT GATCCACCTC CAGCGTACCC CCAGGATCCA 
GCTCCACCAT ACACTGAACC TGGTGAAAGT TTGCCCTTAC CTCCACCTCC AACCTACTAC 
AAATCCTGCC AAAATGTGGA ACAAACTCAA AAAGATACTG AACCTAAGAG GCAAGTCTTT 
TGTGTCCGGC CATGTGATGG TCAAAATGGT TATACCCATG GAAATCCTCA AGGGATCACT 
ATTGGAGGTG TCTGTATTAG AAGAAGATCT GCAGATGGCT CGGATGTCCA GCAGTTCAGG 
ATTATGCCAT ATAGCGAGGA TGGGCACAAT GGGCACAATT GGTCTGTCCA GCAATGCTCA 
GACAGAGGTC CTGGTGCACA AAGACTAATT TTTCAGCTCC AAAAGGACCA AGGACGGCAA 
CCAAGCTCTA CCTCTCAGCC TGACGTGAGT GATTTAGGCA TCACAGGTTC TAAAGGTTCC 
CGCTTGGTGC GGTACCCCAG GATTCGACTT GAGCGGAGCA CGTCACAGCC ATTGCAGCCT 
AGAGGAGCTC CTTCACGCCT TGGATCAATG CCAAACCTTC ATGCTCCTAT CTCAGAAATG 
GAAACGGAGA GTGATTTTAT TGAGAATGGT GCTCAAGGTT GCACTTTCAA AATTAGGAAG 
GAACAGAATT CCCAACAACC GAAGTTTAAG ATTTATTTCA GCCAGGGCGG CGAGCACAGT 
CCCATCCTCG AAAGGAATAT TAGCATTGGA AAGGCGCCTA CCTGCAGCAA TCCACAGGCT 
CACATTGGTC TTGATCGGCC CAAGAACGGT CATTTCAACA ATGGTCAGAC CAGAGATGAC 
TTCCTGGGGC AGGTGGACGT GCCTCTTACA CACTTACCGA CTGAAGACCC TGCCATGGAG 
CGTCCATACA CGTTTAAAGA CTTTCTCCTG CGTCCAAGAA GTCATAAATC TCGGGTGAAA 
GGTTACCTGA GGCTCAAGAT GGCCTACCTG CCAAAGAATG GAGGACATGA GGAGGAAAGC 
GGTGAAATGA GAGAGGAGGC TGAGGGTTGG GAAGTGACAG ACTCCACAGA TCCAGGATCT 
CAGAGACCTC AGCAGCTCCT GCCTCCTCTT CCTCCGGGCT GGGAAGAGAA AGTGGATAAC 
CTGGGACGGA CGTACTATGT GAACCACAAC AACCGCACCA CACAGTGGAA GAGGCCCAGC 
AGTGTGGATG TGGTTTCAGA AACGGAAAAT GATAATCAAT TGCGGCAGAT TAACCAGGAA 
GCCCACAGAG TTTTCCGCTC TCGACGGCAC ATTAGCGAAG ACCTGGAAAA TGAGCAGCTG 
GACATCAGGG ACATAGACGA CTCATGGGAG CCCATATCAG AGGAAGATCC GACTTTATTA 
ACAGATGGTC TGAACCATTC TCTGTCGGGA CCCTCCTCTC TGGCAATGCC CCTCTCCAGC 
ACTCCAGACT TCTCAGATGA GATCAGCCTT AGATTGTCTC TCGCTCCAGA CACCAACGGA 
GAAATCCCGG GACCCAGCTC TGCTGTGAGT CATCTTTCCT CTCGGCTACG CTCCTCTAGT 
ATGACTGATG GAGTCAGTGA TCAAGCTCAA CCTCCCACAC CAACGAGCTC CCAGACCCGA 
CGAACAAGAG CACAAACCGT CACAGGCGTT GAGGACACCA TGTCTCCTTC GGCCTCTTAC 
GCCTTGACCA CGCCAGGCCT TCCACCAGGC TGGGAGGAAC GCAAAGACCC CAAGGGACGA 
ACGTACTACG TTAATCATAA CAACCGTAGC ACCACATGGA CAAGGCCCAT TCTGCAGCAG 
CACACTGAGG ATGGAGCCAG CACCTCGGTT GCAGCAGTAG GGGGCGCTAC AGCAGCTACA 
CCCCCTGCTT CAACCCCTTC CACCTCCAGC GGCCATCTAA GCGAACCACA AGTGCGCAGA 
CCCCGTAGCC TCAGTTCCCC TACCGTCACT CTCTCTTCCC CACTAGAGGG AGCCAATAAC 
GTCCCTATAC GGCGAGCCGT AAAGGACACA TTCTCCAACC CTCAGTCTCC ACAGCCTTCG 
CCCTACAGCT CACCTAAAAC CCAGCATAAA GCCAACCAGA GCTTCCTGCC ACCGGGATGG 
GAAATGAGGA TCGCACCCAA CGGACGGCCG TTCTTCATCG ACCACAACAG CAGAACCACC 
ACATGGGAGG ATCCCCGCTT GAAGTATCCC GTTCACATGA GGACAAAAGC GTCTCTAGAC 
CCGGGCGACC TCGGCCCACT TCCTAATCTT CCAGAGGAGC CTGGGTGGGA GGAACGAGTG 
CATGCTGATG GAAGAACCTT TTACATCGAT CACAACAACA AAAAAACACA GTGGGAAGAC 
CCTCGTCTCC AGAGTCCTGC CATAACTGGA CCCGCGGTGC CGTATTCAAG AGAATTCAAG 
CAGAAATACG ACTACTTCAG GAAGAAGTTA AAGAAACCGG CAGATATCCC CAACCGCTTT 
GAGATGAAGC TGCACAGGAA TAACATCTTT GAGGAGTCCT ACAGACGAAT CATGTCCCTC 
AAGAGACCTG ACAGCCTGAA GGCCCGTCTG TGGATTGAGT TTGAGTCAGA GAAGGGTCTG 
GATTATGGCG GTGTGGCCCG AGAATGGTTC TTCCTGCTCT CTAAGGAGAT GTTCAACCCT 
TATTATGGGC TGTTCGAGTA CTCGGCCACA GATAACTACA CCCTTCAGAT CAATCCCAAC 
TCTGGCCTGT GTAATGAGGA TCACCTCTCA TACTTCAAGT TCATTGGTCG AGTGGCCGGC 
ATGGCTGTTT ATCATGGCAA ACTCTTGGAC GGTTTCTTCA TTCGACCGTT TTATAAGATG 
ATGCTGGGCA AACAGATCAC ACTGAATGAC ATGGAGTCAG TGGACAGCGA ATACTACAAC 
TCCCTGAAGT GGATTCTGGA AAACGATCCC ACAGAATTAG ACTTACGGTT TTGTATTGAT 
GAGGACAACT TTGGACAGAC ATATCAGGTA GATTTGAAGC CAAGCGGATC AGACATGGTT 
GTAACCAACG ACAACAAGAA GGAATACATC GATCTGGTGA TTCAGTGGAG GTTTGTGAAC 
AGGGTCCAGA AACAGATGAA CGCCTTTTTG GAAGGCTTCA CTGAACTCAT TCCCATCGAC 
TTAATAAAGA TTTTTGATGA AAACGAACTG GAGCTGTTGA TGTGTGGTCT GGGAGATGTG 
GATGTCAATG ACTGGAGGCA ACACACTGTC TATAAGAACG GCTACTGTCC AAATCACCCC 
GTCATCCAGT GGTTCTGGAA GGCTGTGTTG TTAATGGATG CAGAAAAGCG CATCAGGCTT 
CTACAGTTTG TCACTGGAAC ATCCAGAGTG CCAATGAACG GCTTTGCTGA ACTCTACGGT 
TCCAATGGGC CGCAGTTGTT TACTATTGAG CAGTGGGGAA CTCCTGACAA ACTCCCTCGG 
GCTCACACCT GCTTTAATCG CTTGGACCTC CCAATGTATG AAACGTTTGA AGACCTGCGA 
GAGAAGCTTC TGATGGCGGT GGAAAACGCT CAAGGCTTTG AGGGCGTCGA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nedd4l ( XM_021469018.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324693.1 Danio rerio neural precursor cell expressed, developmentally down-regulated 4-like (nedd4l), transcript variant X7, mRNA.

Price & Availability↑

CloneID	ODa151017
Clone ID Related Accession (Same CDS sequence)	XM_021469018.1
Accession Version	XM_021469018.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3825bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase NEDD4-like isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCACC GGCTTCGGTT GTATTTTGGA TCTGGTCGCA GTAACACTGC TCCAGAGTTG 
GAAGAGCTGA ATGGGACAGT GGAGGAGGAA GGTGACATAA GCTACAACAG TACAGGAGCC 
ATGGTGGTCC ATGCACAACC ACGTAACGGA GGACATGCAG AAAGTCCAGA ACCAGGCACA 
GTTGTTGGTT CGGATGCCTC TCTAAAGCGA AGCTCTTCCA TGTTCATCCC ACAGCTCCAG 
GGTCAAGCTG AGTCACGGAC AACCAAGAGC ACCTCTGTTC AAATATCTCT TCAACGCACA 
ACTGCCCCTC ATGAAGATGC ATTACCCTTT GATCCACCTC CAGCGTACCC CCAGGATCCA 
GCTCCACCAT ACACTGAACC TGGTGAAAGT TTGCCCTTAC CTCCACCTCC AACCTACTAC 
AAATCCTGCC AAAATGTGGA ACAAACTCAA AAAGATACTG AACCTAAGAG GCAAGTCTTT 
TGTGTCCGGC CATGTGATGG TCAAAATGGT TATACCCATG GAAATCCTCA AGGGATCACT 
ATTGGAGGTG TCTGTATTAG AAGAAGATCT GCAGATGGCT CGGATGTCCA GCAGTTCAGG 
ATTATGCCAT ATAGCGAGGA TGGGCACAAT GGGCACAATT GGTCTGTCCA GCAATGCTCA 
GACAGAGGTC CTGGTGCACA AAGACTAATT TTTCAGCTCC AAAAGGACCA AGGACGGCAA 
CCAAGCTCTA CCTCTCAGCC TGACGTGAGT GATTTAGGCA TCACAGGTTC TAAAGGTTCC 
CGCTTGGTGC GGTACCCCAG GATTCGACTT GAGCGGAGCA CGTCACAGCC ATTGCAGCCT 
AGAGGAGCTC CTTCACGCCT TGGATCAATG CCAAACCTTC ATGCTCCTAT CTCAGAAATG 
GAAACGGAGA GTGATTTTAT TGAGAATGGT GCTCAAGGTT GCACTTTCAA AATTAGGAAG 
GAACAGAATT CCCAACAACC GAAGTTTAAG ATTTATTTCA GCCAGGGCGG CGAGCACAGT 
CCCATCCTCG AAAGGAATAT TAGCATTGGA AAGGCGCCTA CCTGCAGCAA TCCACAGGCT 
CACATTGGTC TTGATCGGCC CAAGAACGGT CATTTCAACA ATGGTCAGAC CAGAGATGAC 
TTCCTGGGGC AGGTGGACGT GCCTCTTACA CACTTACCGA CTGAAGACCC TGCCATGGAG 
CGTCCATACA CGTTTAAAGA CTTTCTCCTG CGTCCAAGAA GTCATAAATC TCGGGTGAAA 
GGTTACCTGA GGCTCAAGAT GGCCTACCTG CCAAAGAATG GAGGACATGA GGAGGAAAGC 
GGTGAAATGA GAGAGGAGGC TGAGGGTTGG GAAGTGACAG ACTCCACAGA TCCAGGATCT 
CAGAGACCTC AGCAGCTCCT GCCTCCTCTT CCTCCGGGCT GGGAAGAGAA AGTGGATAAC 
CTGGGACGGA CGTACTATGT GAACCACAAC AACCGCACCA CACAGTGGAA GAGGCCCAGC 
AGTGTGGATG TGGTTTCAGA AACGGAAAAT GATAATCAAT TGCGGCAGAT TAACCAGGAA 
GCCCACAGAG TTTTCCGCTC TCGACGGCAC ATTAGCGAAG ACCTGGAAAA TGAGCAGCTG 
GACATCAGGG ACATAGACGA CTCATGGGAG CCCATATCAG AGGAAGATCC GACTTTATTA 
ACAGATGGTC TGAACCATTC TCTGTCGGGA CCCTCCTCTC TGGCAATGCC CCTCTCCAGC 
ACTCCAGACT TCTCAGATGA GATCAGCCTT AGATTGTCTC TCGCTCCAGA CACCAACGGA 
GAAATCCCGG GACCCAGCTC TGCTGTGCAG AGTCATCTTT CCTCTCGGCT ACGCTCCTCT 
AGTATGACTG ATGGAGTCAG TGATCAAGCT CAACCTCCCA CACCAACGCA GAGCTCCCAG 
ACCCGACGAA CAAGAGCACA AACCGTCACA GGCGTTGAGG ACACCATGTC TCCTTCGGCC 
TCTTACGCCT TGACCACGCC AGGCCTTCCA CCAGGCTGGG AGGAACGCAA AGACCCCAAG 
GGACGAACGT ACTACGTTAA TCATAACAAC CGTAGCACCA CATGGACAAG GCCCATTCTG 
CAGCAGCACA CTGAGGATGG AGCCAGCACC TCGGTTGCAG CAGTAGGGGG CGCTACAGCA 
GCTACACCCC CTGCTTCAAC CCCTTCCACC TCCAGCGGCC ATCTAAGCGA ACCACAAGTG 
CGCAGACCCC GTAGCCTCAG TTCCCCTACC GTCACTCTCT CTTCCCCACT AGAGGGAGCC 
AATAACGTCC CTATACGGCG AGCCGTAAAG GACACATTCT CCAACCCTCA GTCTCCACAG 
CCTTCGCCCT ACAGCTCACC TAAAACCCAG CATAAAGCCA ACCAGAGCTT CCTGCCACCG 
GGATGGGAAA TGAGGATCGC ACCCAACGGA CGGCCGTTCT TCATCGACCA CAACAGCAGA 
ACCACCACAT GGGAGGATCC CCGCTTGAAG TATCCCGTTC ACATGAGGAC AAAAGCGTCT 
CTAGACCCGG GCGACCTCGG CCCACTTCCT CCTGGGTGGG AGGAACGAGT GCATGCTGAT 
GGAAGAACCT TTTACATCGA TCACAACAAC AAAAAAACAC AGTGGGAAGA CCCTCGTCTC 
CAGAGTCCTG CCATAACTGG ACCCGCGGTG CCGTATTCAA GAGAATTCAA GCAGAAATAC 
GACTACTTCA GGAAGAAGTT AAAGAAACCG GCAGATATCC CCAACCGCTT TGAGATGAAG 
CTGCACAGGA ATAACATCTT TGAGGAGTCC TACAGACGAA TCATGTCCCT CAAGAGACCT 
GACAGCCTGA AGGCCCGTCT GTGGATTGAG TTTGAGTCAG AGAAGGGTCT GGATTATGGC 
GGTGTGGCCC GAGAATGGTT CTTCCTGCTC TCTAAGGAGA TGTTCAACCC TTATTATGGG 
CTGTTCGAGT ACTCGGCCAC AGATAACTAC ACCCTTCAGA TCAATCCCAA CTCTGGCCTG 
TGTAATGAGG ATCACCTCTC ATACTTCAAG TTCATTGGTC GAGTGGCCGG CATGGCTGTT 
TATCATGGCA AACTCTTGGA CGGTTTCTTC ATTCGACCGT TTTATAAGAT GATGCTGGGC 
AAACAGATCA CACTGAATGA CATGGAGTCA GTGGACAGCG AATACTACAA CTCCCTGAAG 
TGGATTCTGG AAAACGATCC CACAGAATTA GACTTACGGT TTTGTATTGA TGAGGACAAC 
TTTGGACAGA CATATCAGGT AGATTTGAAG CCAAGCGGAT CAGACATGGT TGTAACCAAC 
GACAACAAGA AGGAATACAT CGATCTGGTG ATTCAGTGGA GGTTTGTGAA CAGGGTCCAG 
AAACAGATGA ACGCCTTTTT GGAAGGCTTC ACTGAACTCA TTCCCATCGA CTTAATAAAG 
ATTTTTGATG AAAACGAACT GGAGCTGTTG ATGTGTGGTC TGGGAGATGT GGATGTCAAT 
GACTGGAGGC AACACACTGT CTATAAGAAC GGCTACTGTC CAAATCACCC CGTCATCCAG 
TGGTTCTGGA AGGCTGTGTT GTTAATGGAT GCAGAAAAGC GCATCAGGCT TCTACAGTTT 
GTCACTGGAA CATCCAGAGT GCCAATGAAC GGCTTTGCTG AACTCTACGG TTCCAATGGG 
CCGCAGTTGT TTACTATTGA GCAGTGGGGA ACTCCTGACA AACTCCCTCG GGCTCACACC 
TGCTTTAATC GCTTGGACCT CCCAATGTAT GAAACGTTTG AAGACCTGCG AGAGAAGCTT 
CTGATGGCGG TGGAAAACGC TCAAGGCTTT GAGGGCGTCG ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324693.1
CDS	339..4163
Translation	MSHRLRLYFGSGRSNTAPELEELNGTVEEEGDISYNSTGAMVVHAQPRNGGHAESPEPGTVVGSDASLKRSSSMFIPQLQGQAESRTTKSTSVQISLQRTTAPHEDALPFDPPPAYPQDPAPPYTEPGESLPLPPPPTYYKSCQNVEQTQKDTEPKRQVFCVRPCDGQNGYTHGNPQGITIGGVCIRRRSADGSDVQQFRIMPYSEDGHNGHNWSVQQCSDRGPGAQRLIFQLQKDQGRQPSSTSQPDVSDLGITGSKGSRLVRYPRIRLERSTSQPLQPRGAPSRLGSMPNLHAPISEMETESDFIENGAQGCTFKIRKEQNSQQPKFKIYFSQGGEHSPILERNISIGKAPTCSNPQAHIGLDRPKNGHFNNGQTRDDFLGQVDVPLTHLPTEDPAMERPYTFKDFLLRPRSHKSRVKGYLRLKMAYLPKNGGHEEESGEMREEAEGWEVTDSTDPGSQRPQQLLPPLPPGWEEKVDNLGRTYYVNHNNRTTQWKRPSSVDVVSETENDNQLRQINQEAHRVFRSRRHISEDLENEQLDIRDIDDSWEPISEEDPTLLTDGLNHSLSGPSSLAMPLSSTPDFSDEISLRLSLAPDTNGEIPGPSSAVQSHLSSRLRSSSMTDGVSDQAQPPTPTQSSQTRRTRAQTVTGVEDTMSPSASYALTTPGLPPGWEERKDPKGRTYYVNHNNRSTTWTRPILQQHTEDGASTSVAAVGGATAATPPASTPSTSSGHLSEPQVRRPRSLSSPTVTLSSPLEGANNVPIRRAVKDTFSNPQSPQPSPYSSPKTQHKANQSFLPPGWEMRIAPNGRPFFIDHNSRTTTWEDPRLKYPVHMRTKASLDPGDLGPLPPGWEERVHADGRTFYIDHNNKKTQWEDPRLQSPAITGPAVPYSREFKQKYDYFRKKLKKPADIPNRFEMKLHRNNIFEESYRRIMSLKRPDSLKARLWIEFESEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFKFIGRVAGMAVYHGKLLDGFFIRPFYKMMLGKQITLNDMESVDSEYYNSLKWILENDPTELDLRFCIDEDNFGQTYQVDLKPSGSDMVVTNDNKKEYIDLVIQWRFVNRVQKQMNAFLEGFTELIPIDLIKIFDENELELLMCGLGDVDVNDWRQHTVYKNGYCPNHPVIQWFWKAVLLMDAEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGTPDKLPRAHTCFNRLDLPMYETFEDLREKLLMAVENAQGFEGVD

Target ORF information:

RefSeq Version	XM_021469018.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio neural precursor cell expressed, developmentally down-regulated 4-like (nedd4l), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021469018.1

ORF Insert Sequence:

ATGTCTCACC GGCTTCGGTT GTATTTTGGA TCTGGTCGCA GTAACACTGC TCCAGAGTTG 
GAAGAGCTGA ATGGGACAGT GGAGGAGGAA GGTGACATAA GCTACAACAG TACAGGAGCC 
ATGGTGGTCC ATGCACAACC ACGTAACGGA GGACATGCAG AAAGTCCAGA ACCAGGCACA 
GTTGTTGGTT CGGATGCCTC TCTAAAGCGA AGCTCTTCCA TGTTCATCCC ACAGCTCCAG 
GGTCAAGCTG AGTCACGGAC AACCAAGAGC ACCTCTGTTC AAATATCTCT TCAACGCACA 
ACTGCCCCTC ATGAAGATGC ATTACCCTTT GATCCACCTC CAGCGTACCC CCAGGATCCA 
GCTCCACCAT ACACTGAACC TGGTGAAAGT TTGCCCTTAC CTCCACCTCC AACCTACTAC 
AAATCCTGCC AAAATGTGGA ACAAACTCAA AAAGATACTG AACCTAAGAG GCAAGTCTTT 
TGTGTCCGGC CATGTGATGG TCAAAATGGT TATACCCATG GAAATCCTCA AGGGATCACT 
ATTGGAGGTG TCTGTATTAG AAGAAGATCT GCAGATGGCT CGGATGTCCA GCAGTTCAGG 
ATTATGCCAT ATAGCGAGGA TGGGCACAAT GGGCACAATT GGTCTGTCCA GCAATGCTCA 
GACAGAGGTC CTGGTGCACA AAGACTAATT TTTCAGCTCC AAAAGGACCA AGGACGGCAA 
CCAAGCTCTA CCTCTCAGCC TGACGTGAGT GATTTAGGCA TCACAGGTTC TAAAGGTTCC 
CGCTTGGTGC GGTACCCCAG GATTCGACTT GAGCGGAGCA CGTCACAGCC ATTGCAGCCT 
AGAGGAGCTC CTTCACGCCT TGGATCAATG CCAAACCTTC ATGCTCCTAT CTCAGAAATG 
GAAACGGAGA GTGATTTTAT TGAGAATGGT GCTCAAGGTT GCACTTTCAA AATTAGGAAG 
GAACAGAATT CCCAACAACC GAAGTTTAAG ATTTATTTCA GCCAGGGCGG CGAGCACAGT 
CCCATCCTCG AAAGGAATAT TAGCATTGGA AAGGCGCCTA CCTGCAGCAA TCCACAGGCT 
CACATTGGTC TTGATCGGCC CAAGAACGGT CATTTCAACA ATGGTCAGAC CAGAGATGAC 
TTCCTGGGGC AGGTGGACGT GCCTCTTACA CACTTACCGA CTGAAGACCC TGCCATGGAG 
CGTCCATACA CGTTTAAAGA CTTTCTCCTG CGTCCAAGAA GTCATAAATC TCGGGTGAAA 
GGTTACCTGA GGCTCAAGAT GGCCTACCTG CCAAAGAATG GAGGACATGA GGAGGAAAGC 
GGTGAAATGA GAGAGGAGGC TGAGGGTTGG GAAGTGACAG ACTCCACAGA TCCAGGATCT 
CAGAGACCTC AGCAGCTCCT GCCTCCTCTT CCTCCGGGCT GGGAAGAGAA AGTGGATAAC 
CTGGGACGGA CGTACTATGT GAACCACAAC AACCGCACCA CACAGTGGAA GAGGCCCAGC 
AGTGTGGATG TGGTTTCAGA AACGGAAAAT GATAATCAAT TGCGGCAGAT TAACCAGGAA 
GCCCACAGAG TTTTCCGCTC TCGACGGCAC ATTAGCGAAG ACCTGGAAAA TGAGCAGCTG 
GACATCAGGG ACATAGACGA CTCATGGGAG CCCATATCAG AGGAAGATCC GACTTTATTA 
ACAGATGGTC TGAACCATTC TCTGTCGGGA CCCTCCTCTC TGGCAATGCC CCTCTCCAGC 
ACTCCAGACT TCTCAGATGA GATCAGCCTT AGATTGTCTC TCGCTCCAGA CACCAACGGA 
GAAATCCCGG GACCCAGCTC TGCTGTGCAG AGTCATCTTT CCTCTCGGCT ACGCTCCTCT 
AGTATGACTG ATGGAGTCAG TGATCAAGCT CAACCTCCCA CACCAACGCA GAGCTCCCAG 
ACCCGACGAA CAAGAGCACA AACCGTCACA GGCGTTGAGG ACACCATGTC TCCTTCGGCC 
TCTTACGCCT TGACCACGCC AGGCCTTCCA CCAGGCTGGG AGGAACGCAA AGACCCCAAG 
GGACGAACGT ACTACGTTAA TCATAACAAC CGTAGCACCA CATGGACAAG GCCCATTCTG 
CAGCAGCACA CTGAGGATGG AGCCAGCACC TCGGTTGCAG CAGTAGGGGG CGCTACAGCA 
GCTACACCCC CTGCTTCAAC CCCTTCCACC TCCAGCGGCC ATCTAAGCGA ACCACAAGTG 
CGCAGACCCC GTAGCCTCAG TTCCCCTACC GTCACTCTCT CTTCCCCACT AGAGGGAGCC 
AATAACGTCC CTATACGGCG AGCCGTAAAG GACACATTCT CCAACCCTCA GTCTCCACAG 
CCTTCGCCCT ACAGCTCACC TAAAACCCAG CATAAAGCCA ACCAGAGCTT CCTGCCACCG 
GGATGGGAAA TGAGGATCGC ACCCAACGGA CGGCCGTTCT TCATCGACCA CAACAGCAGA 
ACCACCACAT GGGAGGATCC CCGCTTGAAG TATCCCGTTC ACATGAGGAC AAAAGCGTCT 
CTAGACCCGG GCGACCTCGG CCCACTTCCT CCTGGGTGGG AGGAACGAGT GCATGCTGAT 
GGAAGAACCT TTTACATCGA TCACAACAAC AAAAAAACAC AGTGGGAAGA CCCTCGTCTC 
CAGAGTCCTG CCATAACTGG ACCCGCGGTG CCGTATTCAA GAGAATTCAA GCAGAAATAC 
GACTACTTCA GGAAGAAGTT AAAGAAACCG GCAGATATCC CCAACCGCTT TGAGATGAAG 
CTGCACAGGA ATAACATCTT TGAGGAGTCC TACAGACGAA TCATGTCCCT CAAGAGACCT 
GACAGCCTGA AGGCCCGTCT GTGGATTGAG TTTGAGTCAG AGAAGGGTCT GGATTATGGC 
GGTGTGGCCC GAGAATGGTT CTTCCTGCTC TCTAAGGAGA TGTTCAACCC TTATTATGGG 
CTGTTCGAGT ACTCGGCCAC AGATAACTAC ACCCTTCAGA TCAATCCCAA CTCTGGCCTG 
TGTAATGAGG ATCACCTCTC ATACTTCAAG TTCATTGGTC GAGTGGCCGG CATGGCTGTT 
TATCATGGCA AACTCTTGGA CGGTTTCTTC ATTCGACCGT TTTATAAGAT GATGCTGGGC 
AAACAGATCA CACTGAATGA CATGGAGTCA GTGGACAGCG AATACTACAA CTCCCTGAAG 
TGGATTCTGG AAAACGATCC CACAGAATTA GACTTACGGT TTTGTATTGA TGAGGACAAC 
TTTGGACAGA CATATCAGGT AGATTTGAAG CCAAGCGGAT CAGACATGGT TGTAACCAAC 
GACAACAAGA AGGAATACAT CGATCTGGTG ATTCAGTGGA GGTTTGTGAA CAGGGTCCAG 
AAACAGATGA ACGCCTTTTT GGAAGGCTTC ACTGAACTCA TTCCCATCGA CTTAATAAAG 
ATTTTTGATG AAAACGAACT GGAGCTGTTG ATGTGTGGTC TGGGAGATGT GGATGTCAAT 
GACTGGAGGC AACACACTGT CTATAAGAAC GGCTACTGTC CAAATCACCC CGTCATCCAG 
TGGTTCTGGA AGGCTGTGTT GTTAATGGAT GCAGAAAAGC GCATCAGGCT TCTACAGTTT 
GTCACTGGAA CATCCAGAGT GCCAATGAAC GGCTTTGCTG AACTCTACGG TTCCAATGGG 
CCGCAGTTGT TTACTATTGA GCAGTGGGGA ACTCCTGACA AACTCCCTCG GGCTCACACC 
TGCTTTAATC GCTTGGACCT CCCAATGTAT GAAACGTTTG AAGACCTGCG AGAGAAGCTT 
CTGATGGCGG TGGAAAACGC TCAAGGCTTT GAGGGCGTCG ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nedd4l ( XM_021469019.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324694.1 Danio rerio neural precursor cell expressed, developmentally down-regulated 4-like (nedd4l), transcript variant X8, mRNA.

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CloneID	ODa151018
Clone ID Related Accession (Same CDS sequence)	XM_021469019.1
Accession Version	XM_021469019.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3822bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase NEDD4-like isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCACC GGCTTCGGTT GTATTTTGGA TCTGGTCGCA GTAACACTGC TCCAGAGTTG 
GAAGAGCTGA ATGGGACAGT GGAGGAGGAA GGTGACATAA GCTACAACAG TACAGGAGCC 
ATGGTGGTCC ATGCACAACC ACGTAACGGA GGACATGCAG AAAGTCCAGA ACCAGGCACA 
GTTGTTGGTT CGGATGCCTC TCTAAAGCGA AGCTCTTCCA TGTTCATCCC ACAGCTCCAG 
GGTCAAGCTG AGTCACGGAC AACCAAGAGC ACCTCTGTTC AAATATCTCT TCAACGCACA 
ACTGCCCCTC ATGAAGATGC ATTACCCTTT GATCCACCTC CAGCGTACCC CCAGGATCCA 
GCTCCACCAT ACACTGAACC TGGTGAAAGT TTGCCCTTAC CTCCACCTCC AACCTACTAC 
AAATCCTGCC AAAATGTGGA ACAAACTCAA AAAGATACTG AACCTAAGAG GCAAGTCTTT 
TGTGTCCGGC CATGTGATGG TCAAAATGGT TATACCCATG GAAATCCTCA AGGGATCACT 
ATTGGAGGTG TCTGTATTAG AAGAAGATCT GCAGATGGCT CGGATGTCCA GCAGTTCAGG 
ATTATGCCAT ATAGCGAGGA TGGGCACAAT GGGCACAATT GGTCTGTCCA GCAATGCTCA 
GACAGAGGTC CTGGTGCACA AAGACTAATT TTTCAGCTCC AAAAGGACCA AGGACGGCAA 
CCAAGCTCTA CCTCTCAGCC TGACGTGAGT GATTTAGGCA TCACAGGTTC TAAAGGTTCC 
CGCTTGGTGC GGTACCCCAG GATTCGACTT GAGCGGAGCA CGTCACAGCC ATTGCAGCCT 
AGAGGAGCTC CTTCACGCCT TGGATCAATG CCAAACCTTC ATGCTCCTAT CTCAGAAATG 
GAAACGGAGA GTGATTTTAT TGAGAATGGT GCTCAAGGTT GCACTTTCAA AATTAGGAAG 
GAACAGAATT CCCAACAACC GAAGTTTAAG ATTTATTTCA GCCAGGGCGG CGAGCACAGT 
CCCATCCTCG AAAGGAATAT TAGCATTGGA AAGGCGCCTA CCTGCAGCAA TCCACAGGCT 
CACATTGGTC TTGATCGGCC CAAGAACGGT CATTTCAACA ATGGTCAGAC CAGAGATGAC 
TTCCTGGGGC AGGTGGACGT GCCTCTTACA CACTTACCGA CTGAAGACCC TGCCATGGAG 
CGTCCATACA CGTTTAAAGA CTTTCTCCTG CGTCCAAGAA GTCATAAATC TCGGGTGAAA 
GGTTACCTGA GGCTCAAGAT GGCCTACCTG CCAAAGAATG GAGGACATGA GGAGGAAAGC 
GGTGAAATGA GAGAGGAGGC TGAGGGTTGG GAAGTGACAG ACTCCACAGA TCCAGGATCT 
CAGAGACCTC AGCAGCTCCT GCCTCCTCTT CCTCCGGGCT GGGAAGAGAA AGTGGATAAC 
CTGGGACGGA CGTACTATGT GAACCACAAC AACCGCACCA CACAGTGGAA GAGGCCCAGC 
AGTGTGGATG TGGTTTCAGA AACGGAAAAT GATAATCAAT TGCGGCAGAT TAACCAGGAA 
GCCCACAGAG TTTTCCGCTC TCGACGGCAC ATTAGCGAAG ACCTGGAAAA TGAGCAGCTG 
GACATCAGGG ACATAGACGA CTCATGGGAG CCCATATCAG AGGAAGATCC GACTTTATTA 
ACAGATGGTC TGAACCATTC TCTGTCGGGA CCCTCCTCTC TGGCAATGCC CCTCTCCAGC 
ACTCCAGACT TCTCAGATGA GATCAGCCTT AGATTGTCTC TCGCTCCAGA CACCAACGGA 
GAAATCCCGG GACCCAGCTC TGCTGTGCAG AGTCATCTTT CCTCTCGGCT ACGCTCCTCT 
AGTATGACTG ATGGAGTCAG TGATCAAGCT CAACCTCCCA CACCAACGCA GAGCTCCCAG 
ACCCGACGAA CAAGAGCACA AACCGTCACA GGCGTTGAGG ACACCATGTC TCCTTCGGCC 
TCTTACGCCT TGACCACGCC AGGCCTTCCA CCAGGCTGGG AGGAACGCAA AGACCCCAAG 
GGACGAACGT ACTACGTTAA TCATAACAAC CGTAGCACCA CATGGACAAG GCCCATTCTG 
CAGCACACTG AGGATGGAGC CAGCACCTCG GTTGCAGCAG TAGGGGGCGC TACAGCAGCT 
ACACCCCCTG CTTCAACCCC TTCCACCTCC AGCGGCCATC TAAGCGAACC ACAAGTGCGC 
AGACCCCGTA GCCTCAGTTC CCCTACCGTC ACTCTCTCTT CCCCACTAGA GGGAGCCAAT 
AACGTCCCTA TACGGCGAGC CGTAAAGGAC ACATTCTCCA ACCCTCAGTC TCCACAGCCT 
TCGCCCTACA GCTCACCTAA AACCCAGCAT AAAGCCAACC AGAGCTTCCT GCCACCGGGA 
TGGGAAATGA GGATCGCACC CAACGGACGG CCGTTCTTCA TCGACCACAA CAGCAGAACC 
ACCACATGGG AGGATCCCCG CTTGAAGTAT CCCGTTCACA TGAGGACAAA AGCGTCTCTA 
GACCCGGGCG ACCTCGGCCC ACTTCCTCCT GGGTGGGAGG AACGAGTGCA TGCTGATGGA 
AGAACCTTTT ACATCGATCA CAACAACAAA AAAACACAGT GGGAAGACCC TCGTCTCCAG 
AGTCCTGCCA TAACTGGACC CGCGGTGCCG TATTCAAGAG AATTCAAGCA GAAATACGAC 
TACTTCAGGA AGAAGTTAAA GAAACCGGCA GATATCCCCA ACCGCTTTGA GATGAAGCTG 
CACAGGAATA ACATCTTTGA GGAGTCCTAC AGACGAATCA TGTCCCTCAA GAGACCTGAC 
AGCCTGAAGG CCCGTCTGTG GATTGAGTTT GAGTCAGAGA AGGGTCTGGA TTATGGCGGT 
GTGGCCCGAG AATGGTTCTT CCTGCTCTCT AAGGAGATGT TCAACCCTTA TTATGGGCTG 
TTCGAGTACT CGGCCACAGA TAACTACACC CTTCAGATCA ATCCCAACTC TGGCCTGTGT 
AATGAGGATC ACCTCTCATA CTTCAAGTTC ATTGGTCGAG TGGCCGGCAT GGCTGTTTAT 
CATGGCAAAC TCTTGGACGG TTTCTTCATT CGACCGTTTT ATAAGATGAT GCTGGGCAAA 
CAGATCACAC TGAATGACAT GGAGTCAGTG GACAGCGAAT ACTACAACTC CCTGAAGTGG 
ATTCTGGAAA ACGATCCCAC AGAATTAGAC TTACGGTTTT GTATTGATGA GGACAACTTT 
GGACAGACAT ATCAGGTAGA TTTGAAGCCA AGCGGATCAG ACATGGTTGT AACCAACGAC 
AACAAGAAGG AATACATCGA TCTGGTGATT CAGTGGAGGT TTGTGAACAG GGTCCAGAAA 
CAGATGAACG CCTTTTTGGA AGGCTTCACT GAACTCATTC CCATCGACTT AATAAAGATT 
TTTGATGAAA ACGAACTGGA GCTGTTGATG TGTGGTCTGG GAGATGTGGA TGTCAATGAC 
TGGAGGCAAC ACACTGTCTA TAAGAACGGC TACTGTCCAA ATCACCCCGT CATCCAGTGG 
TTCTGGAAGG CTGTGTTGTT AATGGATGCA GAAAAGCGCA TCAGGCTTCT ACAGTTTGTC 
ACTGGAACAT CCAGAGTGCC AATGAACGGC TTTGCTGAAC TCTACGGTTC CAATGGGCCG 
CAGTTGTTTA CTATTGAGCA GTGGGGAACT CCTGACAAAC TCCCTCGGGC TCACACCTGC 
TTTAATCGCT TGGACCTCCC AATGTATGAA ACGTTTGAAG ACCTGCGAGA GAAGCTTCTG 
ATGGCGGTGG AAAACGCTCA AGGCTTTGAG GGCGTCGACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324694.1
CDS	337..4158
Translation	MSHRLRLYFGSGRSNTAPELEELNGTVEEEGDISYNSTGAMVVHAQPRNGGHAESPEPGTVVGSDASLKRSSSMFIPQLQGQAESRTTKSTSVQISLQRTTAPHEDALPFDPPPAYPQDPAPPYTEPGESLPLPPPPTYYKSCQNVEQTQKDTEPKRQVFCVRPCDGQNGYTHGNPQGITIGGVCIRRRSADGSDVQQFRIMPYSEDGHNGHNWSVQQCSDRGPGAQRLIFQLQKDQGRQPSSTSQPDVSDLGITGSKGSRLVRYPRIRLERSTSQPLQPRGAPSRLGSMPNLHAPISEMETESDFIENGAQGCTFKIRKEQNSQQPKFKIYFSQGGEHSPILERNISIGKAPTCSNPQAHIGLDRPKNGHFNNGQTRDDFLGQVDVPLTHLPTEDPAMERPYTFKDFLLRPRSHKSRVKGYLRLKMAYLPKNGGHEEESGEMREEAEGWEVTDSTDPGSQRPQQLLPPLPPGWEEKVDNLGRTYYVNHNNRTTQWKRPSSVDVVSETENDNQLRQINQEAHRVFRSRRHISEDLENEQLDIRDIDDSWEPISEEDPTLLTDGLNHSLSGPSSLAMPLSSTPDFSDEISLRLSLAPDTNGEIPGPSSAVQSHLSSRLRSSSMTDGVSDQAQPPTPTQSSQTRRTRAQTVTGVEDTMSPSASYALTTPGLPPGWEERKDPKGRTYYVNHNNRSTTWTRPILQHTEDGASTSVAAVGGATAATPPASTPSTSSGHLSEPQVRRPRSLSSPTVTLSSPLEGANNVPIRRAVKDTFSNPQSPQPSPYSSPKTQHKANQSFLPPGWEMRIAPNGRPFFIDHNSRTTTWEDPRLKYPVHMRTKASLDPGDLGPLPPGWEERVHADGRTFYIDHNNKKTQWEDPRLQSPAITGPAVPYSREFKQKYDYFRKKLKKPADIPNRFEMKLHRNNIFEESYRRIMSLKRPDSLKARLWIEFESEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFKFIGRVAGMAVYHGKLLDGFFIRPFYKMMLGKQITLNDMESVDSEYYNSLKWILENDPTELDLRFCIDEDNFGQTYQVDLKPSGSDMVVTNDNKKEYIDLVIQWRFVNRVQKQMNAFLEGFTELIPIDLIKIFDENELELLMCGLGDVDVNDWRQHTVYKNGYCPNHPVIQWFWKAVLLMDAEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGTPDKLPRAHTCFNRLDLPMYETFEDLREKLLMAVENAQGFEGVD

Target ORF information:

RefSeq Version	XM_021469019.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio neural precursor cell expressed, developmentally down-regulated 4-like (nedd4l), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021469019.1

ORF Insert Sequence:

ATGTCTCACC GGCTTCGGTT GTATTTTGGA TCTGGTCGCA GTAACACTGC TCCAGAGTTG 
GAAGAGCTGA ATGGGACAGT GGAGGAGGAA GGTGACATAA GCTACAACAG TACAGGAGCC 
ATGGTGGTCC ATGCACAACC ACGTAACGGA GGACATGCAG AAAGTCCAGA ACCAGGCACA 
GTTGTTGGTT CGGATGCCTC TCTAAAGCGA AGCTCTTCCA TGTTCATCCC ACAGCTCCAG 
GGTCAAGCTG AGTCACGGAC AACCAAGAGC ACCTCTGTTC AAATATCTCT TCAACGCACA 
ACTGCCCCTC ATGAAGATGC ATTACCCTTT GATCCACCTC CAGCGTACCC CCAGGATCCA 
GCTCCACCAT ACACTGAACC TGGTGAAAGT TTGCCCTTAC CTCCACCTCC AACCTACTAC 
AAATCCTGCC AAAATGTGGA ACAAACTCAA AAAGATACTG AACCTAAGAG GCAAGTCTTT 
TGTGTCCGGC CATGTGATGG TCAAAATGGT TATACCCATG GAAATCCTCA AGGGATCACT 
ATTGGAGGTG TCTGTATTAG AAGAAGATCT GCAGATGGCT CGGATGTCCA GCAGTTCAGG 
ATTATGCCAT ATAGCGAGGA TGGGCACAAT GGGCACAATT GGTCTGTCCA GCAATGCTCA 
GACAGAGGTC CTGGTGCACA AAGACTAATT TTTCAGCTCC AAAAGGACCA AGGACGGCAA 
CCAAGCTCTA CCTCTCAGCC TGACGTGAGT GATTTAGGCA TCACAGGTTC TAAAGGTTCC 
CGCTTGGTGC GGTACCCCAG GATTCGACTT GAGCGGAGCA CGTCACAGCC ATTGCAGCCT 
AGAGGAGCTC CTTCACGCCT TGGATCAATG CCAAACCTTC ATGCTCCTAT CTCAGAAATG 
GAAACGGAGA GTGATTTTAT TGAGAATGGT GCTCAAGGTT GCACTTTCAA AATTAGGAAG 
GAACAGAATT CCCAACAACC GAAGTTTAAG ATTTATTTCA GCCAGGGCGG CGAGCACAGT 
CCCATCCTCG AAAGGAATAT TAGCATTGGA AAGGCGCCTA CCTGCAGCAA TCCACAGGCT 
CACATTGGTC TTGATCGGCC CAAGAACGGT CATTTCAACA ATGGTCAGAC CAGAGATGAC 
TTCCTGGGGC AGGTGGACGT GCCTCTTACA CACTTACCGA CTGAAGACCC TGCCATGGAG 
CGTCCATACA CGTTTAAAGA CTTTCTCCTG CGTCCAAGAA GTCATAAATC TCGGGTGAAA 
GGTTACCTGA GGCTCAAGAT GGCCTACCTG CCAAAGAATG GAGGACATGA GGAGGAAAGC 
GGTGAAATGA GAGAGGAGGC TGAGGGTTGG GAAGTGACAG ACTCCACAGA TCCAGGATCT 
CAGAGACCTC AGCAGCTCCT GCCTCCTCTT CCTCCGGGCT GGGAAGAGAA AGTGGATAAC 
CTGGGACGGA CGTACTATGT GAACCACAAC AACCGCACCA CACAGTGGAA GAGGCCCAGC 
AGTGTGGATG TGGTTTCAGA AACGGAAAAT GATAATCAAT TGCGGCAGAT TAACCAGGAA 
GCCCACAGAG TTTTCCGCTC TCGACGGCAC ATTAGCGAAG ACCTGGAAAA TGAGCAGCTG 
GACATCAGGG ACATAGACGA CTCATGGGAG CCCATATCAG AGGAAGATCC GACTTTATTA 
ACAGATGGTC TGAACCATTC TCTGTCGGGA CCCTCCTCTC TGGCAATGCC CCTCTCCAGC 
ACTCCAGACT TCTCAGATGA GATCAGCCTT AGATTGTCTC TCGCTCCAGA CACCAACGGA 
GAAATCCCGG GACCCAGCTC TGCTGTGCAG AGTCATCTTT CCTCTCGGCT ACGCTCCTCT 
AGTATGACTG ATGGAGTCAG TGATCAAGCT CAACCTCCCA CACCAACGCA GAGCTCCCAG 
ACCCGACGAA CAAGAGCACA AACCGTCACA GGCGTTGAGG ACACCATGTC TCCTTCGGCC 
TCTTACGCCT TGACCACGCC AGGCCTTCCA CCAGGCTGGG AGGAACGCAA AGACCCCAAG 
GGACGAACGT ACTACGTTAA TCATAACAAC CGTAGCACCA CATGGACAAG GCCCATTCTG 
CAGCACACTG AGGATGGAGC CAGCACCTCG GTTGCAGCAG TAGGGGGCGC TACAGCAGCT 
ACACCCCCTG CTTCAACCCC TTCCACCTCC AGCGGCCATC TAAGCGAACC ACAAGTGCGC 
AGACCCCGTA GCCTCAGTTC CCCTACCGTC ACTCTCTCTT CCCCACTAGA GGGAGCCAAT 
AACGTCCCTA TACGGCGAGC CGTAAAGGAC ACATTCTCCA ACCCTCAGTC TCCACAGCCT 
TCGCCCTACA GCTCACCTAA AACCCAGCAT AAAGCCAACC AGAGCTTCCT GCCACCGGGA 
TGGGAAATGA GGATCGCACC CAACGGACGG CCGTTCTTCA TCGACCACAA CAGCAGAACC 
ACCACATGGG AGGATCCCCG CTTGAAGTAT CCCGTTCACA TGAGGACAAA AGCGTCTCTA 
GACCCGGGCG ACCTCGGCCC ACTTCCTCCT GGGTGGGAGG AACGAGTGCA TGCTGATGGA 
AGAACCTTTT ACATCGATCA CAACAACAAA AAAACACAGT GGGAAGACCC TCGTCTCCAG 
AGTCCTGCCA TAACTGGACC CGCGGTGCCG TATTCAAGAG AATTCAAGCA GAAATACGAC 
TACTTCAGGA AGAAGTTAAA GAAACCGGCA GATATCCCCA ACCGCTTTGA GATGAAGCTG 
CACAGGAATA ACATCTTTGA GGAGTCCTAC AGACGAATCA TGTCCCTCAA GAGACCTGAC 
AGCCTGAAGG CCCGTCTGTG GATTGAGTTT GAGTCAGAGA AGGGTCTGGA TTATGGCGGT 
GTGGCCCGAG AATGGTTCTT CCTGCTCTCT AAGGAGATGT TCAACCCTTA TTATGGGCTG 
TTCGAGTACT CGGCCACAGA TAACTACACC CTTCAGATCA ATCCCAACTC TGGCCTGTGT 
AATGAGGATC ACCTCTCATA CTTCAAGTTC ATTGGTCGAG TGGCCGGCAT GGCTGTTTAT 
CATGGCAAAC TCTTGGACGG TTTCTTCATT CGACCGTTTT ATAAGATGAT GCTGGGCAAA 
CAGATCACAC TGAATGACAT GGAGTCAGTG GACAGCGAAT ACTACAACTC CCTGAAGTGG 
ATTCTGGAAA ACGATCCCAC AGAATTAGAC TTACGGTTTT GTATTGATGA GGACAACTTT 
GGACAGACAT ATCAGGTAGA TTTGAAGCCA AGCGGATCAG ACATGGTTGT AACCAACGAC 
AACAAGAAGG AATACATCGA TCTGGTGATT CAGTGGAGGT TTGTGAACAG GGTCCAGAAA 
CAGATGAACG CCTTTTTGGA AGGCTTCACT GAACTCATTC CCATCGACTT AATAAAGATT 
TTTGATGAAA ACGAACTGGA GCTGTTGATG TGTGGTCTGG GAGATGTGGA TGTCAATGAC 
TGGAGGCAAC ACACTGTCTA TAAGAACGGC TACTGTCCAA ATCACCCCGT CATCCAGTGG 
TTCTGGAAGG CTGTGTTGTT AATGGATGCA GAAAAGCGCA TCAGGCTTCT ACAGTTTGTC 
ACTGGAACAT CCAGAGTGCC AATGAACGGC TTTGCTGAAC TCTACGGTTC CAATGGGCCG 
CAGTTGTTTA CTATTGAGCA GTGGGGAACT CCTGACAAAC TCCCTCGGGC TCACACCTGC 
TTTAATCGCT TGGACCTCCC AATGTATGAA ACGTTTGAAG ACCTGCGAGA GAAGCTTCTG 
ATGGCGGTGG AAAACGCTCA AGGCTTTGAG GGCGTCGACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nedd4l ( XM_021469020.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324695.1 Danio rerio neural precursor cell expressed, developmentally down-regulated 4-like (nedd4l), transcript variant X9, mRNA.

Price & Availability↑

CloneID	ODa151019
Clone ID Related Accession (Same CDS sequence)	XM_021469020.1
Accession Version	XM_021469020.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3789bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase NEDD4-like isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCACC GGCTTCGGTT GTATTTTGGA TCTGGTCGCA GTAACACTGC TCCAGAGTTG 
GAAGAGCTGA ATGGGACAGT GGAGGAGGAA GGTGACATAA GCTACAACAG TACAGGAGCC 
ATGGTGGTCC ATGCACAACC ACGTAACGGA GGACATGCAG AAAGTCCAGA ACCAGGCACA 
GTTGTTGGTT CGGATGCCTC TCTAAAGCGA AGCTCTTCCA TGTTCATCCC ACAGCTCCAG 
GGTCAAGCTG AGTCACGGAC AACCAAGAGC ACCTCTGTTC AAATATCTCT TCAACGCACA 
ACTGCCCCTC ATGAAGATGC ATTACCCTTT GATCCACCTC CAGCGTACCC CCAGGATCCA 
GCTCCACCAT ACACTGAACC TGGTGAAAGT TTGCCCTTAC CTCCACCTCC AACCTACTAC 
AAATCCTGCC AAAATGTGGA ACAAACTCAA AAAGATACTG AACCTAAGAG GCAAGTCTTT 
TGTGTCCGGC CATGTGATGG TCAAAATGGT TATACCCATG GAAATCCTCA AGGGATCACT 
ATTGGAGGTG TCTGTATTAG AAGAAGATCT GCAGATGGCT CGGATGTCCA GCAGTTCAGG 
ATTATGCCAT ATAGCGAGGA TGGGCACAAT GGGCACAATT GGTCTGTCCA GCAATGCTCA 
GACAGAGGTC CTGGTGCACA AAGACTAATT TTTCAGCTCC AAAAGGACCA AGGACGGCAA 
CCAAGCTCTA CCTCTCAGCC TGACGTGAGT GATTTAGGCA TCACAGGTTC TAAAGGTTCC 
CGCTTGGTGC GGTACCCCAG GATTCGACTT GAGCGGAGCA CGTCACAGCC ATTGCAGCCT 
AGAGGAGCTC CTTCACGCCT TGGATCAATG CCAAACCTTC ATGCTCCTAT CTCAGAAATG 
GAAACGGAGA GTGATTTTAT TGAGAATGGT GCTCAAGGTT GCACTTTCAA AATTAGGAAG 
GAACAGAATT CCCAACAACC GAAGTTTAAG ATTTATTTCA GCCAGGGCGG CGAGCACAGT 
CCCATCCTCG AAAGGAATAT TAGCATTGGA AAGGCGCCTA CCTGCAGCAA TCCACAGACC 
AGAGATGACT TCCTGGGGCA GGTGGACGTG CCTCTTACAC ACTTACCGAC TGAAGACCCT 
GCCATGGAGC GTCCATACAC GTTTAAAGAC TTTCTCCTGC GTCCAAGAAG TCATAAATCT 
CGGGTGAAAG GTTACCTGAG GCTCAAGATG GCCTACCTGC CAAAGAATGG AGGACATGAG 
GAGGAAAGCG GTGAAATGAG AGAGGAGGCT GAGGGTTGGG AAGTGACAGA CTCCACAGAT 
CCAGGATCTC AGAGACCTCA GCAGCTCCTG CCTCCTCTTC CTCCGGGCTG GGAAGAGAAA 
GTGGATAACC TGGGACGGAC GTACTATGTG AACCACAACA ACCGCACCAC ACAGTGGAAG 
AGGCCCAGCA GTGTGGATGT GGTTTCAGAA ACGGAAAATG ATAATCAATT GCGGCAGATT 
AACCAGGAAG CCCACAGAGT TTTCCGCTCT CGACGGCACA TTAGCGAAGA CCTGGAAAAT 
GAGCAGCTGG ACATCAGGGA CATAGACGAC TCATGGGAGC CCATATCAGA GGAAGATCCG 
ACTTTATTAA CAGATGGTCT GAACCATTCT CTGTCGGGAC CCTCCTCTCT GGCAATGCCC 
CTCTCCAGCA CTCCAGACTT CTCAGATGAG ATCAGCCTTA GATTGTCTCT CGCTCCAGAC 
ACCAACGGAG AAATCCCGGG ACCCAGCTCT GCTGTGCAGA GTCATCTTTC CTCTCGGCTA 
CGCTCCTCTA GTATGACTGA TGGAGTCAGT GATCAAGCTC AACCTCCCAC ACCAACGCAG 
AGCTCCCAGA CCCGACGAAC AAGAGCACAA ACCGTCACAG GCGTTGAGGA CACCATGTCT 
CCTTCGGCCT CTTACGCCTT GACCACGCCA GGCCTTCCAC CAGGCTGGGA GGAACGCAAA 
GACCCCAAGG GACGAACGTA CTACGTTAAT CATAACAACC GTAGCACCAC ATGGACAAGG 
CCCATTCTGC AGCAGCACAC TGAGGATGGA GCCAGCACCT CGGTTGCAGC AGTAGGGGGC 
GCTACAGCAG CTACACCCCC TGCTTCAACC CCTTCCACCT CCAGCGGCCA TCTAAGCGAA 
CCACAAGTGC GCAGACCCCG TAGCCTCAGT TCCCCTACCG TCACTCTCTC TTCCCCACTA 
GAGGGAGCCA ATAACGTCCC TATACGGCGA GCCGTAAAGG ACACATTCTC CAACCCTCAG 
TCTCCACAGC CTTCGCCCTA CAGCTCACCT AAAACCCAGC ATAAAGCCAA CCAGAGCTTC 
CTGCCACCGG GATGGGAAAT GAGGATCGCA CCCAACGGAC GGCCGTTCTT CATCGACCAC 
AACAGCAGAA CCACCACATG GGAGGATCCC CGCTTGAAGT ATCCCGTTCA CATGAGGACA 
AAAGCGTCTC TAGACCCGGG CGACCTCGGC CCACTTCCTA ATCTTCCAGA GGAGCCTGGG 
TGGGAGGAAC GAGTGCATGC TGATGGAAGA ACCTTTTACA TCGATCACAA CAACAAAAAA 
ACACAGTGGG AAGACCCTCG TCTCCAGAGT CCTGCCATAA CTGGACCCGC GGTGCCGTAT 
TCAAGAGAAT TCAAGCAGAA ATACGACTAC TTCAGGAAGA AGTTAAAGAA ACCGGCAGAT 
ATCCCCAACC GCTTTGAGAT GAAGCTGCAC AGGAATAACA TCTTTGAGGA GTCCTACAGA 
CGAATCATGT CCCTCAAGAG ACCTGACAGC CTGAAGGCCC GTCTGTGGAT TGAGTTTGAG 
TCAGAGAAGG GTCTGGATTA TGGCGGTGTG GCCCGAGAAT GGTTCTTCCT GCTCTCTAAG 
GAGATGTTCA ACCCTTATTA TGGGCTGTTC GAGTACTCGG CCACAGATAA CTACACCCTT 
CAGATCAATC CCAACTCTGG CCTGTGTAAT GAGGATCACC TCTCATACTT CAAGTTCATT 
GGTCGAGTGG CCGGCATGGC TGTTTATCAT GGCAAACTCT TGGACGGTTT CTTCATTCGA 
CCGTTTTATA AGATGATGCT GGGCAAACAG ATCACACTGA ATGACATGGA GTCAGTGGAC 
AGCGAATACT ACAACTCCCT GAAGTGGATT CTGGAAAACG ATCCCACAGA ATTAGACTTA 
CGGTTTTGTA TTGATGAGGA CAACTTTGGA CAGACATATC AGGTAGATTT GAAGCCAAGC 
GGATCAGACA TGGTTGTAAC CAACGACAAC AAGAAGGAAT ACATCGATCT GGTGATTCAG 
TGGAGGTTTG TGAACAGGGT CCAGAAACAG ATGAACGCCT TTTTGGAAGG CTTCACTGAA 
CTCATTCCCA TCGACTTAAT AAAGATTTTT GATGAAAACG AACTGGAGCT GTTGATGTGT 
GGTCTGGGAG ATGTGGATGT CAATGACTGG AGGCAACACA CTGTCTATAA GAACGGCTAC 
TGTCCAAATC ACCCCGTCAT CCAGTGGTTC TGGAAGGCTG TGTTGTTAAT GGATGCAGAA 
AAGCGCATCA GGCTTCTACA GTTTGTCACT GGAACATCCA GAGTGCCAAT GAACGGCTTT 
GCTGAACTCT ACGGTTCCAA TGGGCCGCAG TTGTTTACTA TTGAGCAGTG GGGAACTCCT 
GACAAACTCC CTCGGGCTCA CACCTGCTTT AATCGCTTGG ACCTCCCAAT GTATGAAACG 
TTTGAAGACC TGCGAGAGAA GCTTCTGATG GCGGTGGAAA ACGCTCAAGG CTTTGAGGGC 
GTCGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324695.1
CDS	342..4130
Translation	MSHRLRLYFGSGRSNTAPELEELNGTVEEEGDISYNSTGAMVVHAQPRNGGHAESPEPGTVVGSDASLKRSSSMFIPQLQGQAESRTTKSTSVQISLQRTTAPHEDALPFDPPPAYPQDPAPPYTEPGESLPLPPPPTYYKSCQNVEQTQKDTEPKRQVFCVRPCDGQNGYTHGNPQGITIGGVCIRRRSADGSDVQQFRIMPYSEDGHNGHNWSVQQCSDRGPGAQRLIFQLQKDQGRQPSSTSQPDVSDLGITGSKGSRLVRYPRIRLERSTSQPLQPRGAPSRLGSMPNLHAPISEMETESDFIENGAQGCTFKIRKEQNSQQPKFKIYFSQGGEHSPILERNISIGKAPTCSNPQTRDDFLGQVDVPLTHLPTEDPAMERPYTFKDFLLRPRSHKSRVKGYLRLKMAYLPKNGGHEEESGEMREEAEGWEVTDSTDPGSQRPQQLLPPLPPGWEEKVDNLGRTYYVNHNNRTTQWKRPSSVDVVSETENDNQLRQINQEAHRVFRSRRHISEDLENEQLDIRDIDDSWEPISEEDPTLLTDGLNHSLSGPSSLAMPLSSTPDFSDEISLRLSLAPDTNGEIPGPSSAVQSHLSSRLRSSSMTDGVSDQAQPPTPTQSSQTRRTRAQTVTGVEDTMSPSASYALTTPGLPPGWEERKDPKGRTYYVNHNNRSTTWTRPILQQHTEDGASTSVAAVGGATAATPPASTPSTSSGHLSEPQVRRPRSLSSPTVTLSSPLEGANNVPIRRAVKDTFSNPQSPQPSPYSSPKTQHKANQSFLPPGWEMRIAPNGRPFFIDHNSRTTTWEDPRLKYPVHMRTKASLDPGDLGPLPNLPEEPGWEERVHADGRTFYIDHNNKKTQWEDPRLQSPAITGPAVPYSREFKQKYDYFRKKLKKPADIPNRFEMKLHRNNIFEESYRRIMSLKRPDSLKARLWIEFESEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFKFIGRVAGMAVYHGKLLDGFFIRPFYKMMLGKQITLNDMESVDSEYYNSLKWILENDPTELDLRFCIDEDNFGQTYQVDLKPSGSDMVVTNDNKKEYIDLVIQWRFVNRVQKQMNAFLEGFTELIPIDLIKIFDENELELLMCGLGDVDVNDWRQHTVYKNGYCPNHPVIQWFWKAVLLMDAEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGTPDKLPRAHTCFNRLDLPMYETFEDLREKLLMAVENAQGFEGVD

Target ORF information:

RefSeq Version	XM_021469020.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio neural precursor cell expressed, developmentally down-regulated 4-like (nedd4l), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021469020.1

ORF Insert Sequence:

ATGTCTCACC GGCTTCGGTT GTATTTTGGA TCTGGTCGCA GTAACACTGC TCCAGAGTTG 
GAAGAGCTGA ATGGGACAGT GGAGGAGGAA GGTGACATAA GCTACAACAG TACAGGAGCC 
ATGGTGGTCC ATGCACAACC ACGTAACGGA GGACATGCAG AAAGTCCAGA ACCAGGCACA 
GTTGTTGGTT CGGATGCCTC TCTAAAGCGA AGCTCTTCCA TGTTCATCCC ACAGCTCCAG 
GGTCAAGCTG AGTCACGGAC AACCAAGAGC ACCTCTGTTC AAATATCTCT TCAACGCACA 
ACTGCCCCTC ATGAAGATGC ATTACCCTTT GATCCACCTC CAGCGTACCC CCAGGATCCA 
GCTCCACCAT ACACTGAACC TGGTGAAAGT TTGCCCTTAC CTCCACCTCC AACCTACTAC 
AAATCCTGCC AAAATGTGGA ACAAACTCAA AAAGATACTG AACCTAAGAG GCAAGTCTTT 
TGTGTCCGGC CATGTGATGG TCAAAATGGT TATACCCATG GAAATCCTCA AGGGATCACT 
ATTGGAGGTG TCTGTATTAG AAGAAGATCT GCAGATGGCT CGGATGTCCA GCAGTTCAGG 
ATTATGCCAT ATAGCGAGGA TGGGCACAAT GGGCACAATT GGTCTGTCCA GCAATGCTCA 
GACAGAGGTC CTGGTGCACA AAGACTAATT TTTCAGCTCC AAAAGGACCA AGGACGGCAA 
CCAAGCTCTA CCTCTCAGCC TGACGTGAGT GATTTAGGCA TCACAGGTTC TAAAGGTTCC 
CGCTTGGTGC GGTACCCCAG GATTCGACTT GAGCGGAGCA CGTCACAGCC ATTGCAGCCT 
AGAGGAGCTC CTTCACGCCT TGGATCAATG CCAAACCTTC ATGCTCCTAT CTCAGAAATG 
GAAACGGAGA GTGATTTTAT TGAGAATGGT GCTCAAGGTT GCACTTTCAA AATTAGGAAG 
GAACAGAATT CCCAACAACC GAAGTTTAAG ATTTATTTCA GCCAGGGCGG CGAGCACAGT 
CCCATCCTCG AAAGGAATAT TAGCATTGGA AAGGCGCCTA CCTGCAGCAA TCCACAGACC 
AGAGATGACT TCCTGGGGCA GGTGGACGTG CCTCTTACAC ACTTACCGAC TGAAGACCCT 
GCCATGGAGC GTCCATACAC GTTTAAAGAC TTTCTCCTGC GTCCAAGAAG TCATAAATCT 
CGGGTGAAAG GTTACCTGAG GCTCAAGATG GCCTACCTGC CAAAGAATGG AGGACATGAG 
GAGGAAAGCG GTGAAATGAG AGAGGAGGCT GAGGGTTGGG AAGTGACAGA CTCCACAGAT 
CCAGGATCTC AGAGACCTCA GCAGCTCCTG CCTCCTCTTC CTCCGGGCTG GGAAGAGAAA 
GTGGATAACC TGGGACGGAC GTACTATGTG AACCACAACA ACCGCACCAC ACAGTGGAAG 
AGGCCCAGCA GTGTGGATGT GGTTTCAGAA ACGGAAAATG ATAATCAATT GCGGCAGATT 
AACCAGGAAG CCCACAGAGT TTTCCGCTCT CGACGGCACA TTAGCGAAGA CCTGGAAAAT 
GAGCAGCTGG ACATCAGGGA CATAGACGAC TCATGGGAGC CCATATCAGA GGAAGATCCG 
ACTTTATTAA CAGATGGTCT GAACCATTCT CTGTCGGGAC CCTCCTCTCT GGCAATGCCC 
CTCTCCAGCA CTCCAGACTT CTCAGATGAG ATCAGCCTTA GATTGTCTCT CGCTCCAGAC 
ACCAACGGAG AAATCCCGGG ACCCAGCTCT GCTGTGCAGA GTCATCTTTC CTCTCGGCTA 
CGCTCCTCTA GTATGACTGA TGGAGTCAGT GATCAAGCTC AACCTCCCAC ACCAACGCAG 
AGCTCCCAGA CCCGACGAAC AAGAGCACAA ACCGTCACAG GCGTTGAGGA CACCATGTCT 
CCTTCGGCCT CTTACGCCTT GACCACGCCA GGCCTTCCAC CAGGCTGGGA GGAACGCAAA 
GACCCCAAGG GACGAACGTA CTACGTTAAT CATAACAACC GTAGCACCAC ATGGACAAGG 
CCCATTCTGC AGCAGCACAC TGAGGATGGA GCCAGCACCT CGGTTGCAGC AGTAGGGGGC 
GCTACAGCAG CTACACCCCC TGCTTCAACC CCTTCCACCT CCAGCGGCCA TCTAAGCGAA 
CCACAAGTGC GCAGACCCCG TAGCCTCAGT TCCCCTACCG TCACTCTCTC TTCCCCACTA 
GAGGGAGCCA ATAACGTCCC TATACGGCGA GCCGTAAAGG ACACATTCTC CAACCCTCAG 
TCTCCACAGC CTTCGCCCTA CAGCTCACCT AAAACCCAGC ATAAAGCCAA CCAGAGCTTC 
CTGCCACCGG GATGGGAAAT GAGGATCGCA CCCAACGGAC GGCCGTTCTT CATCGACCAC 
AACAGCAGAA CCACCACATG GGAGGATCCC CGCTTGAAGT ATCCCGTTCA CATGAGGACA 
AAAGCGTCTC TAGACCCGGG CGACCTCGGC CCACTTCCTA ATCTTCCAGA GGAGCCTGGG 
TGGGAGGAAC GAGTGCATGC TGATGGAAGA ACCTTTTACA TCGATCACAA CAACAAAAAA 
ACACAGTGGG AAGACCCTCG TCTCCAGAGT CCTGCCATAA CTGGACCCGC GGTGCCGTAT 
TCAAGAGAAT TCAAGCAGAA ATACGACTAC TTCAGGAAGA AGTTAAAGAA ACCGGCAGAT 
ATCCCCAACC GCTTTGAGAT GAAGCTGCAC AGGAATAACA TCTTTGAGGA GTCCTACAGA 
CGAATCATGT CCCTCAAGAG ACCTGACAGC CTGAAGGCCC GTCTGTGGAT TGAGTTTGAG 
TCAGAGAAGG GTCTGGATTA TGGCGGTGTG GCCCGAGAAT GGTTCTTCCT GCTCTCTAAG 
GAGATGTTCA ACCCTTATTA TGGGCTGTTC GAGTACTCGG CCACAGATAA CTACACCCTT 
CAGATCAATC CCAACTCTGG CCTGTGTAAT GAGGATCACC TCTCATACTT CAAGTTCATT 
GGTCGAGTGG CCGGCATGGC TGTTTATCAT GGCAAACTCT TGGACGGTTT CTTCATTCGA 
CCGTTTTATA AGATGATGCT GGGCAAACAG ATCACACTGA ATGACATGGA GTCAGTGGAC 
AGCGAATACT ACAACTCCCT GAAGTGGATT CTGGAAAACG ATCCCACAGA ATTAGACTTA 
CGGTTTTGTA TTGATGAGGA CAACTTTGGA CAGACATATC AGGTAGATTT GAAGCCAAGC 
GGATCAGACA TGGTTGTAAC CAACGACAAC AAGAAGGAAT ACATCGATCT GGTGATTCAG 
TGGAGGTTTG TGAACAGGGT CCAGAAACAG ATGAACGCCT TTTTGGAAGG CTTCACTGAA 
CTCATTCCCA TCGACTTAAT AAAGATTTTT GATGAAAACG AACTGGAGCT GTTGATGTGT 
GGTCTGGGAG ATGTGGATGT CAATGACTGG AGGCAACACA CTGTCTATAA GAACGGCTAC 
TGTCCAAATC ACCCCGTCAT CCAGTGGTTC TGGAAGGCTG TGTTGTTAAT GGATGCAGAA 
AAGCGCATCA GGCTTCTACA GTTTGTCACT GGAACATCCA GAGTGCCAAT GAACGGCTTT 
GCTGAACTCT ACGGTTCCAA TGGGCCGCAG TTGTTTACTA TTGAGCAGTG GGGAACTCCT 
GACAAACTCC CTCGGGCTCA CACCTGCTTT AATCGCTTGG ACCTCCCAAT GTATGAAACG 
TTTGAAGACC TGCGAGAGAA GCTTCTGATG GCGGTGGAAA ACGCTCAAGG CTTTGAGGGC 
GTCGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nedd4l ( XM_021469021.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324696.1 Danio rerio neural precursor cell expressed, developmentally down-regulated 4-like (nedd4l), transcript variant X10, mRNA.

Price & Availability↑

CloneID	ODa151020
Clone ID Related Accession (Same CDS sequence)	XM_021469021.1
Accession Version	XM_021469021.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3780bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase NEDD4-like isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCACC GGCTTCGGTT GTATTTTGGA TCTGGTCGCA GTAACACTGC TCCAGAGTTG 
GAAGAGCTGA ATGGGACAGT GGAGGAGGAA GGTGACATAA GCTACAACAG TACAGGAGCC 
ATGGTGGTCC ATGCACAACC ACGTAACGGA GGACATGCAG AAAGTCCAGA ACCAGGCACA 
GTTGTTGGTT CGGATGCCTC TCTAAAGCGA AGCTCTTCCA TGTTCATCCC ACAGCTCCAG 
GGTCAAGCTG AGTCACGGAC AACCAAGAGC ACCTCTGTTC AAATATCTCT TCAACGCACA 
ACTGCCCCTC ATGAAGATGC ATTACCCTTT GATCCACCTC CAGCGTACCC CCAGGATCCA 
GCTCCACCAT ACACTGAACC TGGTGAAAGT TTGCCCTTAC CTCCACCTCC AACCTACTAC 
AAATCCTGCC AAAATGTGGA ACAAACTCAA AAAGATACTG AACCTAAGAG GCAAGTCTTT 
TGTGTCCGGC CATGTGATGG TCAAAATGGT TATACCCATG GAAATCCTCA AGGGATCACT 
ATTGGAGGTG TCTGTATTAG AAGAAGATCT GCAGATGGCT CGGATGTCCA GCAGTTCAGG 
ATTATGCCAT ATAGCGAGGA TGGGCACAAT GGGCACAATT GGTCTGTCCA GCAATGCTCA 
GACAGAGGTC CTGGTGCACA AAGACTAATT TTTCAGCTCC AAAAGGACCA AGGACGGCAA 
CCAAGCTCTA CCTCTCAGCC TGACGTGAGT GATTTAGGCA TCACAGGTTC TAAAGGTTCC 
CGCTTGGTGC GGTACCCCAG GATTCGACTT GAGCGGAGCA CGTCACAGCC ATTGCAGCCT 
AGAGGAGCTC CTTCACGCCT TGGATCAATG CCAAACCTTC ATGCTCCTAT CTCAGAAATG 
GAAACGGAGA GTGATTTTAT TGAGAATGGT GCTCAAGGTT GCACTTTCAA AATTAGGAAG 
GAACAGAATT CCCAACAACC GAAGTTTAAG ATTTATTTCA GCCAGGGCGG CGAGCACAGT 
CCCATCCTCG AAAGGAATAT TAGCATTGGA AAGGCGCCTA CCTGCAGCAA TCCACAGGCT 
CACATTGGTC TTGATCGGCC CAAGAACGGT CATTTCAACA ATGGTCAGAC CAGAGATGAC 
TTCCTGGGGC AGGTGGACGT GCCTCTTACA CACTTACCGA CTGAAGACCC TGCCATGGAG 
CGTCCATACA CGTTTAAAGA CTTTCTCCTG CGTCCAAGAA GTCATAAATC TCGGGTGAAA 
GGTTACCTGA GGCTCAAGAT GGCCTACCTG CCAAAGAATG GAGGACATGA GGAGGAAAGC 
GGTGAAATGA GAGAGGAGGC TGAGGGTTGG GAAGTGACAG ACTCCACAGA TCCAGGATCT 
CAGAGACCTC AGCAGCTCCT GCCTCCTCTT CCTCCGGGCT GGGAAGAGAA AGTGGATAAC 
CTGGGACGGA CGTACTATGT GAACCACAAC AACCGCACCA CACAGTGGAA GAGGCCCAGC 
AGTGTGGATG TGGTTTCAGA AACGGAAAAT GATAATCAAT TGCGGCAGAT TAACCAGGAA 
GCCCACAGAG TTTTCCGCTC TCGACGGCAC ATTAGCGAAG ACCTGGAAAA TGAGCAGCTG 
GACATCAGGG ACATAGACGA CTCATGGGAG CCCATATCAG AGGAAGATCC GACTTTATTA 
ACAGATGGTC TGAACCATTC TCTGTCGGGA CCCTCCTCTC TGGCAATGCC CCTCTCCAGC 
ACTCCAGACT TCTCAGATGA GATCAGCCTT AGATTGTCTC TCGCTCCAGA CACCAACGGA 
GAAATCCCGG GACCCAGCTC TGCTGTGCAG AGTCATCTTT CCTCTCGGCT ACGCTCCTCT 
AGTATGACTG ATGGAGTCAG TGATCAAGCT CAACCTCCCA CACCAACGTC TCCTTCGGCC 
TCTTACGCCT TGACCACGCC AGGCCTTCCA CCAGGCTGGG AGGAACGCAA AGACCCCAAG 
GGACGAACGT ACTACGTTAA TCATAACAAC CGTAGCACCA CATGGACAAG GCCCATTCTG 
CAGCAGCACA CTGAGGATGG AGCCAGCACC TCGGTTGCAG CAGTAGGGGG CGCTACAGCA 
GCTACACCCC CTGCTTCAAC CCCTTCCACC TCCAGCGGCC ATCTAAGCGA ACCACAAGTG 
CGCAGACCCC GTAGCCTCAG TTCCCCTACC GTCACTCTCT CTTCCCCACT AGAGGGAGCC 
AATAACGTCC CTATACGGCG AGCCGTAAAG GACACATTCT CCAACCCTCA GTCTCCACAG 
CCTTCGCCCT ACAGCTCACC TAAAACCCAG CATAAAGCCA ACCAGAGCTT CCTGCCACCG 
GGATGGGAAA TGAGGATCGC ACCCAACGGA CGGCCGTTCT TCATCGACCA CAACAGCAGA 
ACCACCACAT GGGAGGATCC CCGCTTGAAG TATCCCGTTC ACATGAGGAC AAAAGCGTCT 
CTAGACCCGG GCGACCTCGG CCCACTTCCT AATCTTCCAG AGGAGCCTGG GTGGGAGGAA 
CGAGTGCATG CTGATGGAAG AACCTTTTAC ATCGATCACA ACAACAAAAA AACACAGTGG 
GAAGACCCTC GTCTCCAGAG TCCTGCCATA ACTGGACCCG CGGTGCCGTA TTCAAGAGAA 
TTCAAGCAGA AATACGACTA CTTCAGGAAG AAGTTAAAGA AACCGGCAGA TATCCCCAAC 
CGCTTTGAGA TGAAGCTGCA CAGGAATAAC ATCTTTGAGG AGTCCTACAG ACGAATCATG 
TCCCTCAAGA GACCTGACAG CCTGAAGGCC CGTCTGTGGA TTGAGTTTGA GTCAGAGAAG 
GGTCTGGATT ATGGCGGTGT GGCCCGAGAA TGGTTCTTCC TGCTCTCTAA GGAGATGTTC 
AACCCTTATT ATGGGCTGTT CGAGTACTCG GCCACAGATA ACTACACCCT TCAGATCAAT 
CCCAACTCTG GCCTGTGTAA TGAGGATCAC CTCTCATACT TCAAGTTCAT TGGTCGAGTG 
GCCGGCATGG CTGTTTATCA TGGCAAACTC TTGGACGGTT TCTTCATTCG ACCGTTTTAT 
AAGATGATGC TGGGCAAACA GATCACACTG AATGACATGG AGTCAGTGGA CAGCGAATAC 
TACAACTCCC TGAAGTGGAT TCTGGAAAAC GATCCCACAG AATTAGACTT ACGGTTTTGT 
ATTGATGAGG ACAACTTTGG ACAGACATAT CAGGTAGATT TGAAGCCAAG CGGATCAGAC 
ATGGTTGTAA CCAACGACAA CAAGAAGGAA TACATCGATC TGGTGATTCA GTGGAGGTTT 
GTGAACAGGG TCCAGAAACA GATGAACGCC TTTTTGGAAG GCTTCACTGA ACTCATTCCC 
ATCGACTTAA TAAAGATTTT TGATGAAAAC GAACTGGAGC TGTTGATGTG TGGTCTGGGA 
GATGTGGATG TCAATGACTG GAGGCAACAC ACTGTCTATA AGAACGGCTA CTGTCCAAAT 
CACCCCGTCA TCCAGTGGTT CTGGAAGGCT GTGTTGTTAA TGGATGCAGA AAAGCGCATC 
AGGCTTCTAC AGTTTGTCAC TGGAACATCC AGAGTGCCAA TGAACGGCTT TGCTGAACTC 
TACGGTTCCA ATGGGCCGCA GTTGTTTACT ATTGAGCAGT GGGGAACTCC TGACAAACTC 
CCTCGGGCTC ACACCTGCTT TAATCGCTTG GACCTCCCAA TGTATGAAAC GTTTGAAGAC 
CTGCGAGAGA AGCTTCTGAT GGCGGTGGAA AACGCTCAAG GCTTTGAGGG CGTCGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324696.1
CDS	340..4119
Translation	MSHRLRLYFGSGRSNTAPELEELNGTVEEEGDISYNSTGAMVVHAQPRNGGHAESPEPGTVVGSDASLKRSSSMFIPQLQGQAESRTTKSTSVQISLQRTTAPHEDALPFDPPPAYPQDPAPPYTEPGESLPLPPPPTYYKSCQNVEQTQKDTEPKRQVFCVRPCDGQNGYTHGNPQGITIGGVCIRRRSADGSDVQQFRIMPYSEDGHNGHNWSVQQCSDRGPGAQRLIFQLQKDQGRQPSSTSQPDVSDLGITGSKGSRLVRYPRIRLERSTSQPLQPRGAPSRLGSMPNLHAPISEMETESDFIENGAQGCTFKIRKEQNSQQPKFKIYFSQGGEHSPILERNISIGKAPTCSNPQAHIGLDRPKNGHFNNGQTRDDFLGQVDVPLTHLPTEDPAMERPYTFKDFLLRPRSHKSRVKGYLRLKMAYLPKNGGHEEESGEMREEAEGWEVTDSTDPGSQRPQQLLPPLPPGWEEKVDNLGRTYYVNHNNRTTQWKRPSSVDVVSETENDNQLRQINQEAHRVFRSRRHISEDLENEQLDIRDIDDSWEPISEEDPTLLTDGLNHSLSGPSSLAMPLSSTPDFSDEISLRLSLAPDTNGEIPGPSSAVQSHLSSRLRSSSMTDGVSDQAQPPTPTSPSASYALTTPGLPPGWEERKDPKGRTYYVNHNNRSTTWTRPILQQHTEDGASTSVAAVGGATAATPPASTPSTSSGHLSEPQVRRPRSLSSPTVTLSSPLEGANNVPIRRAVKDTFSNPQSPQPSPYSSPKTQHKANQSFLPPGWEMRIAPNGRPFFIDHNSRTTTWEDPRLKYPVHMRTKASLDPGDLGPLPNLPEEPGWEERVHADGRTFYIDHNNKKTQWEDPRLQSPAITGPAVPYSREFKQKYDYFRKKLKKPADIPNRFEMKLHRNNIFEESYRRIMSLKRPDSLKARLWIEFESEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFKFIGRVAGMAVYHGKLLDGFFIRPFYKMMLGKQITLNDMESVDSEYYNSLKWILENDPTELDLRFCIDEDNFGQTYQVDLKPSGSDMVVTNDNKKEYIDLVIQWRFVNRVQKQMNAFLEGFTELIPIDLIKIFDENELELLMCGLGDVDVNDWRQHTVYKNGYCPNHPVIQWFWKAVLLMDAEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGTPDKLPRAHTCFNRLDLPMYETFEDLREKLLMAVENAQGFEGVD

Target ORF information:

RefSeq Version	XM_021469021.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio neural precursor cell expressed, developmentally down-regulated 4-like (nedd4l), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021469021.1

ORF Insert Sequence:

ATGTCTCACC GGCTTCGGTT GTATTTTGGA TCTGGTCGCA GTAACACTGC TCCAGAGTTG 
GAAGAGCTGA ATGGGACAGT GGAGGAGGAA GGTGACATAA GCTACAACAG TACAGGAGCC 
ATGGTGGTCC ATGCACAACC ACGTAACGGA GGACATGCAG AAAGTCCAGA ACCAGGCACA 
GTTGTTGGTT CGGATGCCTC TCTAAAGCGA AGCTCTTCCA TGTTCATCCC ACAGCTCCAG 
GGTCAAGCTG AGTCACGGAC AACCAAGAGC ACCTCTGTTC AAATATCTCT TCAACGCACA 
ACTGCCCCTC ATGAAGATGC ATTACCCTTT GATCCACCTC CAGCGTACCC CCAGGATCCA 
GCTCCACCAT ACACTGAACC TGGTGAAAGT TTGCCCTTAC CTCCACCTCC AACCTACTAC 
AAATCCTGCC AAAATGTGGA ACAAACTCAA AAAGATACTG AACCTAAGAG GCAAGTCTTT 
TGTGTCCGGC CATGTGATGG TCAAAATGGT TATACCCATG GAAATCCTCA AGGGATCACT 
ATTGGAGGTG TCTGTATTAG AAGAAGATCT GCAGATGGCT CGGATGTCCA GCAGTTCAGG 
ATTATGCCAT ATAGCGAGGA TGGGCACAAT GGGCACAATT GGTCTGTCCA GCAATGCTCA 
GACAGAGGTC CTGGTGCACA AAGACTAATT TTTCAGCTCC AAAAGGACCA AGGACGGCAA 
CCAAGCTCTA CCTCTCAGCC TGACGTGAGT GATTTAGGCA TCACAGGTTC TAAAGGTTCC 
CGCTTGGTGC GGTACCCCAG GATTCGACTT GAGCGGAGCA CGTCACAGCC ATTGCAGCCT 
AGAGGAGCTC CTTCACGCCT TGGATCAATG CCAAACCTTC ATGCTCCTAT CTCAGAAATG 
GAAACGGAGA GTGATTTTAT TGAGAATGGT GCTCAAGGTT GCACTTTCAA AATTAGGAAG 
GAACAGAATT CCCAACAACC GAAGTTTAAG ATTTATTTCA GCCAGGGCGG CGAGCACAGT 
CCCATCCTCG AAAGGAATAT TAGCATTGGA AAGGCGCCTA CCTGCAGCAA TCCACAGGCT 
CACATTGGTC TTGATCGGCC CAAGAACGGT CATTTCAACA ATGGTCAGAC CAGAGATGAC 
TTCCTGGGGC AGGTGGACGT GCCTCTTACA CACTTACCGA CTGAAGACCC TGCCATGGAG 
CGTCCATACA CGTTTAAAGA CTTTCTCCTG CGTCCAAGAA GTCATAAATC TCGGGTGAAA 
GGTTACCTGA GGCTCAAGAT GGCCTACCTG CCAAAGAATG GAGGACATGA GGAGGAAAGC 
GGTGAAATGA GAGAGGAGGC TGAGGGTTGG GAAGTGACAG ACTCCACAGA TCCAGGATCT 
CAGAGACCTC AGCAGCTCCT GCCTCCTCTT CCTCCGGGCT GGGAAGAGAA AGTGGATAAC 
CTGGGACGGA CGTACTATGT GAACCACAAC AACCGCACCA CACAGTGGAA GAGGCCCAGC 
AGTGTGGATG TGGTTTCAGA AACGGAAAAT GATAATCAAT TGCGGCAGAT TAACCAGGAA 
GCCCACAGAG TTTTCCGCTC TCGACGGCAC ATTAGCGAAG ACCTGGAAAA TGAGCAGCTG 
GACATCAGGG ACATAGACGA CTCATGGGAG CCCATATCAG AGGAAGATCC GACTTTATTA 
ACAGATGGTC TGAACCATTC TCTGTCGGGA CCCTCCTCTC TGGCAATGCC CCTCTCCAGC 
ACTCCAGACT TCTCAGATGA GATCAGCCTT AGATTGTCTC TCGCTCCAGA CACCAACGGA 
GAAATCCCGG GACCCAGCTC TGCTGTGCAG AGTCATCTTT CCTCTCGGCT ACGCTCCTCT 
AGTATGACTG ATGGAGTCAG TGATCAAGCT CAACCTCCCA CACCAACGTC TCCTTCGGCC 
TCTTACGCCT TGACCACGCC AGGCCTTCCA CCAGGCTGGG AGGAACGCAA AGACCCCAAG 
GGACGAACGT ACTACGTTAA TCATAACAAC CGTAGCACCA CATGGACAAG GCCCATTCTG 
CAGCAGCACA CTGAGGATGG AGCCAGCACC TCGGTTGCAG CAGTAGGGGG CGCTACAGCA 
GCTACACCCC CTGCTTCAAC CCCTTCCACC TCCAGCGGCC ATCTAAGCGA ACCACAAGTG 
CGCAGACCCC GTAGCCTCAG TTCCCCTACC GTCACTCTCT CTTCCCCACT AGAGGGAGCC 
AATAACGTCC CTATACGGCG AGCCGTAAAG GACACATTCT CCAACCCTCA GTCTCCACAG 
CCTTCGCCCT ACAGCTCACC TAAAACCCAG CATAAAGCCA ACCAGAGCTT CCTGCCACCG 
GGATGGGAAA TGAGGATCGC ACCCAACGGA CGGCCGTTCT TCATCGACCA CAACAGCAGA 
ACCACCACAT GGGAGGATCC CCGCTTGAAG TATCCCGTTC ACATGAGGAC AAAAGCGTCT 
CTAGACCCGG GCGACCTCGG CCCACTTCCT AATCTTCCAG AGGAGCCTGG GTGGGAGGAA 
CGAGTGCATG CTGATGGAAG AACCTTTTAC ATCGATCACA ACAACAAAAA AACACAGTGG 
GAAGACCCTC GTCTCCAGAG TCCTGCCATA ACTGGACCCG CGGTGCCGTA TTCAAGAGAA 
TTCAAGCAGA AATACGACTA CTTCAGGAAG AAGTTAAAGA AACCGGCAGA TATCCCCAAC 
CGCTTTGAGA TGAAGCTGCA CAGGAATAAC ATCTTTGAGG AGTCCTACAG ACGAATCATG 
TCCCTCAAGA GACCTGACAG CCTGAAGGCC CGTCTGTGGA TTGAGTTTGA GTCAGAGAAG 
GGTCTGGATT ATGGCGGTGT GGCCCGAGAA TGGTTCTTCC TGCTCTCTAA GGAGATGTTC 
AACCCTTATT ATGGGCTGTT CGAGTACTCG GCCACAGATA ACTACACCCT TCAGATCAAT 
CCCAACTCTG GCCTGTGTAA TGAGGATCAC CTCTCATACT TCAAGTTCAT TGGTCGAGTG 
GCCGGCATGG CTGTTTATCA TGGCAAACTC TTGGACGGTT TCTTCATTCG ACCGTTTTAT 
AAGATGATGC TGGGCAAACA GATCACACTG AATGACATGG AGTCAGTGGA CAGCGAATAC 
TACAACTCCC TGAAGTGGAT TCTGGAAAAC GATCCCACAG AATTAGACTT ACGGTTTTGT 
ATTGATGAGG ACAACTTTGG ACAGACATAT CAGGTAGATT TGAAGCCAAG CGGATCAGAC 
ATGGTTGTAA CCAACGACAA CAAGAAGGAA TACATCGATC TGGTGATTCA GTGGAGGTTT 
GTGAACAGGG TCCAGAAACA GATGAACGCC TTTTTGGAAG GCTTCACTGA ACTCATTCCC 
ATCGACTTAA TAAAGATTTT TGATGAAAAC GAACTGGAGC TGTTGATGTG TGGTCTGGGA 
GATGTGGATG TCAATGACTG GAGGCAACAC ACTGTCTATA AGAACGGCTA CTGTCCAAAT 
CACCCCGTCA TCCAGTGGTT CTGGAAGGCT GTGTTGTTAA TGGATGCAGA AAAGCGCATC 
AGGCTTCTAC AGTTTGTCAC TGGAACATCC AGAGTGCCAA TGAACGGCTT TGCTGAACTC 
TACGGTTCCA ATGGGCCGCA GTTGTTTACT ATTGAGCAGT GGGGAACTCC TGACAAACTC 
CCTCGGGCTC ACACCTGCTT TAATCGCTTG GACCTCCCAA TGTATGAAAC GTTTGAAGAC 
CTGCGAGAGA AGCTTCTGAT GGCGGTGGAA AACGCTCAAG GCTTTGAGGG CGTCGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nedd4l ( XM_021469022.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324697.1 Danio rerio neural precursor cell expressed, developmentally down-regulated 4-like (nedd4l), transcript variant X11, mRNA.

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CloneID	ODa151021
Clone ID Related Accession (Same CDS sequence)	XM_021469022.1
Accession Version	XM_021469022.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3777bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase NEDD4-like isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCACC GGCTTCGGTT GTATTTTGGA TCTGGTCGCA GTAACACTGC TCCAGAGTTG 
GAAGAGCTGA ATGGGACAGT GGAGGAGGAA GGTGACATAA GCTACAACAG TACAGGAGCC 
ATGGTGGTCC ATGCACAACC ACGTAACGGA GGACATGCAG AAAGTCCAGA ACCAGGCACA 
GTTGTTGGTT CGGATGCCTC TCTAAAGCGA AGCTCTTCCA TGTTCATCCC ACAGCTCCAG 
GGTCAAGCTG AGTCACGGAC AACCAAGAGC ACCTCTGTTC AAATATCTCT TCAACGCACA 
ACTGCCCCTC ATGAAGATGC ATTACCCTTT GATCCACCTC CAGCGTACCC CCAGGATCCA 
GCTCCACCAT ACACTGAACC TGGTGAAAGT TTGCCCTTAC CTCCACCTCC AACCTACTAC 
AAATCCTGCC AAAATGTGGA ACAAACTCAA AAAGATACTG AACCTAAGAG GCAAGTCTTT 
TGTGTCCGGC CATGTGATGG TCAAAATGGT TATACCCATG GAAATCCTCA AGGGATCACT 
ATTGGAGGTG TCTGTATTAG AAGAAGATCT GCAGATGGCT CGGATGTCCA GCAGTTCAGG 
ATTATGCCAT ATAGCGAGGA TGGGCACAAT GGGCACAATT GGTCTGTCCA GCAATGCTCA 
GACAGAGGTC CTGGTGCACA AAGACTAATT TTTCAGCTCC AAAAGGACCA AGGACGGCAA 
CCAAGCTCTA CCTCTCAGCC TGACGTGAGT GATTTAGGCA TCACAGGTTC TAAAGGTTCC 
CGCTTGGTGC GGTACCCCAG GATTCGACTT GAGCGGAGCA CGTCACAGCC ATTGCAGCCT 
AGAGGAGCTC CTTCACGCCT TGGATCAATG CCAAACCTTC ATGCTCCTAT CTCAGAAATG 
GAAACGGAGA GTGATTTTAT TGAGAATGGT GCTCAAGGTT GCACTTTCAA AATTAGGAAG 
GAACAGAATT CCCAACAACC GAAGTTTAAG ATTTATTTCA GCCAGGGCGG CGAGCACAGT 
CCCATCCTCG AAAGGAATAT TAGCATTGGA AAGGCGCCTA CCTGCAGCAA TCCACAGGCT 
CACATTGGTC TTGATCGGCC CAAGAACGGT CATTTCAACA ATGGTCAGAC CAGAGATGAC 
TTCCTGGGGC AGGTGGACGT GCCTCTTACA CACTTACCGA CTGAAGACCC TGCCATGGAG 
CGTCCATACA CGTTTAAAGA CTTTCTCCTG CGTCCAAGAA GTCATAAATC TCGGGTGAAA 
GGTTACCTGA GGCTCAAGAT GGCCTACCTG CCAAAGAATG GAGGACATGA GGAGGAAAGC 
GGTGAAATGA GAGAGGAGGC TGAGGGTTGG GAAGTGACAG ACTCCACAGA TCCAGGATCT 
CAGAGACCTC AGCAGCTCCT GCCTCCTCTT CCTCCGGGCT GGGAAGAGAA AGTGGATAAC 
CTGGGACGGA CGTACTATGT GAACCACAAC AACCGCACCA CACAGTGGAA GAGGCCCAGC 
AGTGTGGATG TGGTTTCAGA AACGGAAAAT GATAATCAAT TGCGGCAGAT TAACCAGGAA 
GCCCACAGAG TTTTCCGCTC TCGACGGCAC ATTAGCGAAG ACCTGGAAAA TGAGCAGCTG 
GACATCAGGG ACATAGACGA CTCATGGGAG CCCATATCAG AGGAAGATCC GACTTTATTA 
ACAGATGGTC TGAACCATTC TCTGTCGGGA CCCTCCTCTC TGGCAATGCC CCTCTCCAGC 
ACTCCAGACT TCTCAGATGA GATCAGCCTT AGATTGTCTC TCGCTCCAGA CACCAACGGA 
GAAATCCCGG GACCCAGCTC TGCTGTGAGT CATCTTTCCT CTCGGCTACG CTCCTCTAGT 
ATGACTGATG GAGTCAGTGA TCAAGCTCAA CCTCCCACAC CAACGTCTCC TTCGGCCTCT 
TACGCCTTGA CCACGCCAGG CCTTCCACCA GGCTGGGAGG AACGCAAAGA CCCCAAGGGA 
CGAACGTACT ACGTTAATCA TAACAACCGT AGCACCACAT GGACAAGGCC CATTCTGCAG 
CAGCACACTG AGGATGGAGC CAGCACCTCG GTTGCAGCAG TAGGGGGCGC TACAGCAGCT 
ACACCCCCTG CTTCAACCCC TTCCACCTCC AGCGGCCATC TAAGCGAACC ACAAGTGCGC 
AGACCCCGTA GCCTCAGTTC CCCTACCGTC ACTCTCTCTT CCCCACTAGA GGGAGCCAAT 
AACGTCCCTA TACGGCGAGC CGTAAAGGAC ACATTCTCCA ACCCTCAGTC TCCACAGCCT 
TCGCCCTACA GCTCACCTAA AACCCAGCAT AAAGCCAACC AGAGCTTCCT GCCACCGGGA 
TGGGAAATGA GGATCGCACC CAACGGACGG CCGTTCTTCA TCGACCACAA CAGCAGAACC 
ACCACATGGG AGGATCCCCG CTTGAAGTAT CCCGTTCACA TGAGGACAAA AGCGTCTCTA 
GACCCGGGCG ACCTCGGCCC ACTTCCTAAT CTTCCAGAGG AGCCTGGGTG GGAGGAACGA 
GTGCATGCTG ATGGAAGAAC CTTTTACATC GATCACAACA ACAAAAAAAC ACAGTGGGAA 
GACCCTCGTC TCCAGAGTCC TGCCATAACT GGACCCGCGG TGCCGTATTC AAGAGAATTC 
AAGCAGAAAT ACGACTACTT CAGGAAGAAG TTAAAGAAAC CGGCAGATAT CCCCAACCGC 
TTTGAGATGA AGCTGCACAG GAATAACATC TTTGAGGAGT CCTACAGACG AATCATGTCC 
CTCAAGAGAC CTGACAGCCT GAAGGCCCGT CTGTGGATTG AGTTTGAGTC AGAGAAGGGT 
CTGGATTATG GCGGTGTGGC CCGAGAATGG TTCTTCCTGC TCTCTAAGGA GATGTTCAAC 
CCTTATTATG GGCTGTTCGA GTACTCGGCC ACAGATAACT ACACCCTTCA GATCAATCCC 
AACTCTGGCC TGTGTAATGA GGATCACCTC TCATACTTCA AGTTCATTGG TCGAGTGGCC 
GGCATGGCTG TTTATCATGG CAAACTCTTG GACGGTTTCT TCATTCGACC GTTTTATAAG 
ATGATGCTGG GCAAACAGAT CACACTGAAT GACATGGAGT CAGTGGACAG CGAATACTAC 
AACTCCCTGA AGTGGATTCT GGAAAACGAT CCCACAGAAT TAGACTTACG GTTTTGTATT 
GATGAGGACA ACTTTGGACA GACATATCAG GTAGATTTGA AGCCAAGCGG ATCAGACATG 
GTTGTAACCA ACGACAACAA GAAGGAATAC ATCGATCTGG TGATTCAGTG GAGGTTTGTG 
AACAGGGTCC AGAAACAGAT GAACGCCTTT TTGGAAGGCT TCACTGAACT CATTCCCATC 
GACTTAATAA AGATTTTTGA TGAAAACGAA CTGGAGCTGT TGATGTGTGG TCTGGGAGAT 
GTGGATGTCA ATGACTGGAG GCAACACACT GTCTATAAGA ACGGCTACTG TCCAAATCAC 
CCCGTCATCC AGTGGTTCTG GAAGGCTGTG TTGTTAATGG ATGCAGAAAA GCGCATCAGG 
CTTCTACAGT TTGTCACTGG AACATCCAGA GTGCCAATGA ACGGCTTTGC TGAACTCTAC 
GGTTCCAATG GGCCGCAGTT GTTTACTATT GAGCAGTGGG GAACTCCTGA CAAACTCCCT 
CGGGCTCACA CCTGCTTTAA TCGCTTGGAC CTCCCAATGT ATGAAACGTT TGAAGACCTG 
CGAGAGAAGC TTCTGATGGC GGTGGAAAAC GCTCAAGGCT TTGAGGGCGT CGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324697.1
CDS	343..4119
Translation	MSHRLRLYFGSGRSNTAPELEELNGTVEEEGDISYNSTGAMVVHAQPRNGGHAESPEPGTVVGSDASLKRSSSMFIPQLQGQAESRTTKSTSVQISLQRTTAPHEDALPFDPPPAYPQDPAPPYTEPGESLPLPPPPTYYKSCQNVEQTQKDTEPKRQVFCVRPCDGQNGYTHGNPQGITIGGVCIRRRSADGSDVQQFRIMPYSEDGHNGHNWSVQQCSDRGPGAQRLIFQLQKDQGRQPSSTSQPDVSDLGITGSKGSRLVRYPRIRLERSTSQPLQPRGAPSRLGSMPNLHAPISEMETESDFIENGAQGCTFKIRKEQNSQQPKFKIYFSQGGEHSPILERNISIGKAPTCSNPQAHIGLDRPKNGHFNNGQTRDDFLGQVDVPLTHLPTEDPAMERPYTFKDFLLRPRSHKSRVKGYLRLKMAYLPKNGGHEEESGEMREEAEGWEVTDSTDPGSQRPQQLLPPLPPGWEEKVDNLGRTYYVNHNNRTTQWKRPSSVDVVSETENDNQLRQINQEAHRVFRSRRHISEDLENEQLDIRDIDDSWEPISEEDPTLLTDGLNHSLSGPSSLAMPLSSTPDFSDEISLRLSLAPDTNGEIPGPSSAVSHLSSRLRSSSMTDGVSDQAQPPTPTSPSASYALTTPGLPPGWEERKDPKGRTYYVNHNNRSTTWTRPILQQHTEDGASTSVAAVGGATAATPPASTPSTSSGHLSEPQVRRPRSLSSPTVTLSSPLEGANNVPIRRAVKDTFSNPQSPQPSPYSSPKTQHKANQSFLPPGWEMRIAPNGRPFFIDHNSRTTTWEDPRLKYPVHMRTKASLDPGDLGPLPNLPEEPGWEERVHADGRTFYIDHNNKKTQWEDPRLQSPAITGPAVPYSREFKQKYDYFRKKLKKPADIPNRFEMKLHRNNIFEESYRRIMSLKRPDSLKARLWIEFESEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFKFIGRVAGMAVYHGKLLDGFFIRPFYKMMLGKQITLNDMESVDSEYYNSLKWILENDPTELDLRFCIDEDNFGQTYQVDLKPSGSDMVVTNDNKKEYIDLVIQWRFVNRVQKQMNAFLEGFTELIPIDLIKIFDENELELLMCGLGDVDVNDWRQHTVYKNGYCPNHPVIQWFWKAVLLMDAEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGTPDKLPRAHTCFNRLDLPMYETFEDLREKLLMAVENAQGFEGVD

Target ORF information:

RefSeq Version	XM_021469022.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio neural precursor cell expressed, developmentally down-regulated 4-like (nedd4l), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021469022.1

ORF Insert Sequence:

ATGTCTCACC GGCTTCGGTT GTATTTTGGA TCTGGTCGCA GTAACACTGC TCCAGAGTTG 
GAAGAGCTGA ATGGGACAGT GGAGGAGGAA GGTGACATAA GCTACAACAG TACAGGAGCC 
ATGGTGGTCC ATGCACAACC ACGTAACGGA GGACATGCAG AAAGTCCAGA ACCAGGCACA 
GTTGTTGGTT CGGATGCCTC TCTAAAGCGA AGCTCTTCCA TGTTCATCCC ACAGCTCCAG 
GGTCAAGCTG AGTCACGGAC AACCAAGAGC ACCTCTGTTC AAATATCTCT TCAACGCACA 
ACTGCCCCTC ATGAAGATGC ATTACCCTTT GATCCACCTC CAGCGTACCC CCAGGATCCA 
GCTCCACCAT ACACTGAACC TGGTGAAAGT TTGCCCTTAC CTCCACCTCC AACCTACTAC 
AAATCCTGCC AAAATGTGGA ACAAACTCAA AAAGATACTG AACCTAAGAG GCAAGTCTTT 
TGTGTCCGGC CATGTGATGG TCAAAATGGT TATACCCATG GAAATCCTCA AGGGATCACT 
ATTGGAGGTG TCTGTATTAG AAGAAGATCT GCAGATGGCT CGGATGTCCA GCAGTTCAGG 
ATTATGCCAT ATAGCGAGGA TGGGCACAAT GGGCACAATT GGTCTGTCCA GCAATGCTCA 
GACAGAGGTC CTGGTGCACA AAGACTAATT TTTCAGCTCC AAAAGGACCA AGGACGGCAA 
CCAAGCTCTA CCTCTCAGCC TGACGTGAGT GATTTAGGCA TCACAGGTTC TAAAGGTTCC 
CGCTTGGTGC GGTACCCCAG GATTCGACTT GAGCGGAGCA CGTCACAGCC ATTGCAGCCT 
AGAGGAGCTC CTTCACGCCT TGGATCAATG CCAAACCTTC ATGCTCCTAT CTCAGAAATG 
GAAACGGAGA GTGATTTTAT TGAGAATGGT GCTCAAGGTT GCACTTTCAA AATTAGGAAG 
GAACAGAATT CCCAACAACC GAAGTTTAAG ATTTATTTCA GCCAGGGCGG CGAGCACAGT 
CCCATCCTCG AAAGGAATAT TAGCATTGGA AAGGCGCCTA CCTGCAGCAA TCCACAGGCT 
CACATTGGTC TTGATCGGCC CAAGAACGGT CATTTCAACA ATGGTCAGAC CAGAGATGAC 
TTCCTGGGGC AGGTGGACGT GCCTCTTACA CACTTACCGA CTGAAGACCC TGCCATGGAG 
CGTCCATACA CGTTTAAAGA CTTTCTCCTG CGTCCAAGAA GTCATAAATC TCGGGTGAAA 
GGTTACCTGA GGCTCAAGAT GGCCTACCTG CCAAAGAATG GAGGACATGA GGAGGAAAGC 
GGTGAAATGA GAGAGGAGGC TGAGGGTTGG GAAGTGACAG ACTCCACAGA TCCAGGATCT 
CAGAGACCTC AGCAGCTCCT GCCTCCTCTT CCTCCGGGCT GGGAAGAGAA AGTGGATAAC 
CTGGGACGGA CGTACTATGT GAACCACAAC AACCGCACCA CACAGTGGAA GAGGCCCAGC 
AGTGTGGATG TGGTTTCAGA AACGGAAAAT GATAATCAAT TGCGGCAGAT TAACCAGGAA 
GCCCACAGAG TTTTCCGCTC TCGACGGCAC ATTAGCGAAG ACCTGGAAAA TGAGCAGCTG 
GACATCAGGG ACATAGACGA CTCATGGGAG CCCATATCAG AGGAAGATCC GACTTTATTA 
ACAGATGGTC TGAACCATTC TCTGTCGGGA CCCTCCTCTC TGGCAATGCC CCTCTCCAGC 
ACTCCAGACT TCTCAGATGA GATCAGCCTT AGATTGTCTC TCGCTCCAGA CACCAACGGA 
GAAATCCCGG GACCCAGCTC TGCTGTGAGT CATCTTTCCT CTCGGCTACG CTCCTCTAGT 
ATGACTGATG GAGTCAGTGA TCAAGCTCAA CCTCCCACAC CAACGTCTCC TTCGGCCTCT 
TACGCCTTGA CCACGCCAGG CCTTCCACCA GGCTGGGAGG AACGCAAAGA CCCCAAGGGA 
CGAACGTACT ACGTTAATCA TAACAACCGT AGCACCACAT GGACAAGGCC CATTCTGCAG 
CAGCACACTG AGGATGGAGC CAGCACCTCG GTTGCAGCAG TAGGGGGCGC TACAGCAGCT 
ACACCCCCTG CTTCAACCCC TTCCACCTCC AGCGGCCATC TAAGCGAACC ACAAGTGCGC 
AGACCCCGTA GCCTCAGTTC CCCTACCGTC ACTCTCTCTT CCCCACTAGA GGGAGCCAAT 
AACGTCCCTA TACGGCGAGC CGTAAAGGAC ACATTCTCCA ACCCTCAGTC TCCACAGCCT 
TCGCCCTACA GCTCACCTAA AACCCAGCAT AAAGCCAACC AGAGCTTCCT GCCACCGGGA 
TGGGAAATGA GGATCGCACC CAACGGACGG CCGTTCTTCA TCGACCACAA CAGCAGAACC 
ACCACATGGG AGGATCCCCG CTTGAAGTAT CCCGTTCACA TGAGGACAAA AGCGTCTCTA 
GACCCGGGCG ACCTCGGCCC ACTTCCTAAT CTTCCAGAGG AGCCTGGGTG GGAGGAACGA 
GTGCATGCTG ATGGAAGAAC CTTTTACATC GATCACAACA ACAAAAAAAC ACAGTGGGAA 
GACCCTCGTC TCCAGAGTCC TGCCATAACT GGACCCGCGG TGCCGTATTC AAGAGAATTC 
AAGCAGAAAT ACGACTACTT CAGGAAGAAG TTAAAGAAAC CGGCAGATAT CCCCAACCGC 
TTTGAGATGA AGCTGCACAG GAATAACATC TTTGAGGAGT CCTACAGACG AATCATGTCC 
CTCAAGAGAC CTGACAGCCT GAAGGCCCGT CTGTGGATTG AGTTTGAGTC AGAGAAGGGT 
CTGGATTATG GCGGTGTGGC CCGAGAATGG TTCTTCCTGC TCTCTAAGGA GATGTTCAAC 
CCTTATTATG GGCTGTTCGA GTACTCGGCC ACAGATAACT ACACCCTTCA GATCAATCCC 
AACTCTGGCC TGTGTAATGA GGATCACCTC TCATACTTCA AGTTCATTGG TCGAGTGGCC 
GGCATGGCTG TTTATCATGG CAAACTCTTG GACGGTTTCT TCATTCGACC GTTTTATAAG 
ATGATGCTGG GCAAACAGAT CACACTGAAT GACATGGAGT CAGTGGACAG CGAATACTAC 
AACTCCCTGA AGTGGATTCT GGAAAACGAT CCCACAGAAT TAGACTTACG GTTTTGTATT 
GATGAGGACA ACTTTGGACA GACATATCAG GTAGATTTGA AGCCAAGCGG ATCAGACATG 
GTTGTAACCA ACGACAACAA GAAGGAATAC ATCGATCTGG TGATTCAGTG GAGGTTTGTG 
AACAGGGTCC AGAAACAGAT GAACGCCTTT TTGGAAGGCT TCACTGAACT CATTCCCATC 
GACTTAATAA AGATTTTTGA TGAAAACGAA CTGGAGCTGT TGATGTGTGG TCTGGGAGAT 
GTGGATGTCA ATGACTGGAG GCAACACACT GTCTATAAGA ACGGCTACTG TCCAAATCAC 
CCCGTCATCC AGTGGTTCTG GAAGGCTGTG TTGTTAATGG ATGCAGAAAA GCGCATCAGG 
CTTCTACAGT TTGTCACTGG AACATCCAGA GTGCCAATGA ACGGCTTTGC TGAACTCTAC 
GGTTCCAATG GGCCGCAGTT GTTTACTATT GAGCAGTGGG GAACTCCTGA CAAACTCCCT 
CGGGCTCACA CCTGCTTTAA TCGCTTGGAC CTCCCAATGT ATGAAACGTT TGAAGACCTG 
CGAGAGAAGC TTCTGATGGC GGTGGAAAAC GCTCAAGGCT TTGAGGGCGT CGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nedd4l ( XM_021469023.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324698.1 Danio rerio neural precursor cell expressed, developmentally down-regulated 4-like (nedd4l), transcript variant X12, mRNA.

Price & Availability↑

CloneID	ODa151022
Clone ID Related Accession (Same CDS sequence)	XM_021469023.1
Accession Version	XM_021469023.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3057bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase NEDD4-like isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTACGA ATTTTGAACC CATTTATGGA CTTTCAGAGG ATGAAAACGA ATCTCGTATT 
CTTCGCGTCA AGGTCATTGC TGGCATTGAT CTTGCGAAGA AGGACATCAT TGGTGCCAGT 
GATCCATACG TCAAACTCTC CCTGTATGTC GCTGATGAAA ACCGCGAGCT TGCCTTAGTA 
CAAACGAAAA CCATCAAAAA GACTCTTAAT CCCAAATGGA ACGAGGAATT CTATTTCAGA 
GTCTGCCCAC AAAACCACAG GCTTCTTTTC GAGGTGTTTG ACGAAAACCG CTTGGCTCAC 
ATTGGTCTTG ATCGGCCCAA GAACGGTCAT TTCAACAATG GTCAGACCAG AGATGACTTC 
CTGGGGCAGG TGGACGTGCC TCTTACACAC TTACCGACTG AAGACCCTGC CATGGAGCGT 
CCATACACGT TTAAAGACTT TCTCCTGCGT CCAAGAAGTC ATAAATCTCG GGTGAAAGGT 
TACCTGAGGC TCAAGATGGC CTACCTGCCA AAGAATGGAG GACATGAGGA GGAAAGCGGT 
GAAATGAGAG AGGAGGCTGA GGGTTGGGAA GTGACAGACT CCACAGATCC AGGATCTCAG 
AGACCTCAGC AGCTCCTGCC TCCTCTTCCT CCGGGCTGGG AAGAGAAAGT GGATAACCTG 
GGACGGACGT ACTATGTGAA CCACAACAAC CGCACCACAC AGTGGAAGAG GCCCAGCAGT 
GTGGATGTGG TTTCAGAAAC GGAAAATGAT AATCAATTGC GGCAGATTAA CCAGGAAGCC 
CACAGAGTTT TCCGCTCTCG ACGGCACATT AGCGAAGACC TGGAAAATGA GCAGCTGGAC 
ATCAGGGACA TAGACGACTC ATGGGAGCCC ATATCAGAGG AAGATCCGAC TTTATTAACA 
GATGGTCTGA ACCATTCTCT GTCGGGACCC TCCTCTCTGG CAATGCCCCT CTCCAGCACT 
CCAGACTTCT CAGATGAGAT CAGCCTTAGA TTGTCTCTCG CTCCAGACAC CAACGGAGAA 
ATCCCGGGAC CCAGCTCTGC TGTGCAGAGT CATCTTTCCT CTCGGCTACG CTCCTCTAGT 
ATGACTGATG GAGTCAGTGA TCAAGCTCAA CCTCCCACAC CAACGCAGAG CTCCCAGACC 
CGACGAACAA GAGCACAAAC CGTCACAGGC GTTGAGGACA CCATGTCTCC TTCGGCCTCT 
TACGCCTTGA CCACGCCAGG CCTTCCACCA GGCTGGGAGG AACGCAAAGA CCCCAAGGGA 
CGAACGTACT ACGTTAATCA TAACAACCGT AGCACCACAT GGACAAGGCC CATTCTGCAG 
CAGCACACTG AGGATGGAGC CAGCACCTCG GTTGCAGCAG TAGGGGGCGC TACAGCAGCT 
ACACCCCCTG CTTCAACCCC TTCCACCTCC AGCGGCCATC TAAGCGAACC ACAAGTGCGC 
AGACCCCGTA GCCTCAGTTC CCCTACCGTC ACTCTCTCTT CCCCACTAGA GGGAGCCAAT 
AACGTCCCTA TACGGCGAGC CGTAAAGGAC ACATTCTCCA ACCCTCAGTC TCCACAGCCT 
TCGCCCTACA GCTCACCTAA AACCCAGCAT AAAGCCAACC AGAGCTTCCT GCCACCGGGA 
TGGGAAATGA GGATCGCACC CAACGGACGG CCGTTCTTCA TCGACCACAA CAGCAGAACC 
ACCACATGGG AGGATCCCCG CTTGAAGTAT CCCGTTCACA TGAGGACAAA AGCGTCTCTA 
GACCCGGGCG ACCTCGGCCC ACTTCCTAAT CTTCCAGAGG AGCCTGGGTG GGAGGAACGA 
GTGCATGCTG ATGGAAGAAC CTTTTACATC GATCACAACA ACAAAAAAAC ACAGTGGGAA 
GACCCTCGTC TCCAGAGTCC TGCCATAACT GGACCCGCGG TGCCGTATTC AAGAGAATTC 
AAGCAGAAAT ACGACTACTT CAGGAAGAAG TTAAAGAAAC CGGCAGATAT CCCCAACCGC 
TTTGAGATGA AGCTGCACAG GAATAACATC TTTGAGGAGT CCTACAGACG AATCATGTCC 
CTCAAGAGAC CTGACAGCCT GAAGGCCCGT CTGTGGATTG AGTTTGAGTC AGAGAAGGGT 
CTGGATTATG GCGGTGTGGC CCGAGAATGG TTCTTCCTGC TCTCTAAGGA GATGTTCAAC 
CCTTATTATG GGCTGTTCGA GTACTCGGCC ACAGATAACT ACACCCTTCA GATCAATCCC 
AACTCTGGCC TGTGTAATGA GGATCACCTC TCATACTTCA AGTTCATTGG TCGAGTGGCC 
GGCATGGCTG TTTATCATGG CAAACTCTTG GACGGTTTCT TCATTCGACC GTTTTATAAG 
ATGATGCTGG GCAAACAGAT CACACTGAAT GACATGGAGT CAGTGGACAG CGAATACTAC 
AACTCCCTGA AGTGGATTCT GGAAAACGAT CCCACAGAAT TAGACTTACG GTTTTGTATT 
GATGAGGACA ACTTTGGACA GACATATCAG GTAGATTTGA AGCCAAGCGG ATCAGACATG 
GTTGTAACCA ACGACAACAA GAAGGAATAC ATCGATCTGG TGATTCAGTG GAGGTTTGTG 
AACAGGGTCC AGAAACAGAT GAACGCCTTT TTGGAAGGCT TCACTGAACT CATTCCCATC 
GACTTAATAA AGATTTTTGA TGAAAACGAA CTGGAGCTGT TGATGTGTGG TCTGGGAGAT 
GTGGATGTCA ATGACTGGAG GCAACACACT GTCTATAAGA ACGGCTACTG TCCAAATCAC 
CCCGTCATCC AGTGGTTCTG GAAGGCTGTG TTGTTAATGG ATGCAGAAAA GCGCATCAGG 
CTTCTACAGT TTGTCACTGG AACATCCAGA GTGCCAATGA ACGGCTTTGC TGAACTCTAC 
GGTTCCAATG GGCCGCAGTT GTTTACTATT GAGCAGTGGG GAACTCCTGA CAAACTCCCT 
CGGGCTCACA CCTGCTTTAA TCGCTTGGAC CTCCCAATGT ATGAAACGTT TGAAGACCTG 
CGAGAGAAGC TTCTGATGGC GGTGGAAAAC GCTCAAGGCT TTGAGGGCGT CGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324698.1
CDS	304..3360
Translation	MATNFEPIYGLSEDENESRILRVKVIAGIDLAKKDIIGASDPYVKLSLYVADENRELALVQTKTIKKTLNPKWNEEFYFRVCPQNHRLLFEVFDENRLAHIGLDRPKNGHFNNGQTRDDFLGQVDVPLTHLPTEDPAMERPYTFKDFLLRPRSHKSRVKGYLRLKMAYLPKNGGHEEESGEMREEAEGWEVTDSTDPGSQRPQQLLPPLPPGWEEKVDNLGRTYYVNHNNRTTQWKRPSSVDVVSETENDNQLRQINQEAHRVFRSRRHISEDLENEQLDIRDIDDSWEPISEEDPTLLTDGLNHSLSGPSSLAMPLSSTPDFSDEISLRLSLAPDTNGEIPGPSSAVQSHLSSRLRSSSMTDGVSDQAQPPTPTQSSQTRRTRAQTVTGVEDTMSPSASYALTTPGLPPGWEERKDPKGRTYYVNHNNRSTTWTRPILQQHTEDGASTSVAAVGGATAATPPASTPSTSSGHLSEPQVRRPRSLSSPTVTLSSPLEGANNVPIRRAVKDTFSNPQSPQPSPYSSPKTQHKANQSFLPPGWEMRIAPNGRPFFIDHNSRTTTWEDPRLKYPVHMRTKASLDPGDLGPLPNLPEEPGWEERVHADGRTFYIDHNNKKTQWEDPRLQSPAITGPAVPYSREFKQKYDYFRKKLKKPADIPNRFEMKLHRNNIFEESYRRIMSLKRPDSLKARLWIEFESEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFKFIGRVAGMAVYHGKLLDGFFIRPFYKMMLGKQITLNDMESVDSEYYNSLKWILENDPTELDLRFCIDEDNFGQTYQVDLKPSGSDMVVTNDNKKEYIDLVIQWRFVNRVQKQMNAFLEGFTELIPIDLIKIFDENELELLMCGLGDVDVNDWRQHTVYKNGYCPNHPVIQWFWKAVLLMDAEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGTPDKLPRAHTCFNRLDLPMYETFEDLREKLLMAVENAQGFEGVD

Target ORF information:

RefSeq Version	XM_021469023.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio neural precursor cell expressed, developmentally down-regulated 4-like (nedd4l), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021469023.1

ORF Insert Sequence:

ATGGCTACGA ATTTTGAACC CATTTATGGA CTTTCAGAGG ATGAAAACGA ATCTCGTATT 
CTTCGCGTCA AGGTCATTGC TGGCATTGAT CTTGCGAAGA AGGACATCAT TGGTGCCAGT 
GATCCATACG TCAAACTCTC CCTGTATGTC GCTGATGAAA ACCGCGAGCT TGCCTTAGTA 
CAAACGAAAA CCATCAAAAA GACTCTTAAT CCCAAATGGA ACGAGGAATT CTATTTCAGA 
GTCTGCCCAC AAAACCACAG GCTTCTTTTC GAGGTGTTTG ACGAAAACCG CTTGGCTCAC 
ATTGGTCTTG ATCGGCCCAA GAACGGTCAT TTCAACAATG GTCAGACCAG AGATGACTTC 
CTGGGGCAGG TGGACGTGCC TCTTACACAC TTACCGACTG AAGACCCTGC CATGGAGCGT 
CCATACACGT TTAAAGACTT TCTCCTGCGT CCAAGAAGTC ATAAATCTCG GGTGAAAGGT 
TACCTGAGGC TCAAGATGGC CTACCTGCCA AAGAATGGAG GACATGAGGA GGAAAGCGGT 
GAAATGAGAG AGGAGGCTGA GGGTTGGGAA GTGACAGACT CCACAGATCC AGGATCTCAG 
AGACCTCAGC AGCTCCTGCC TCCTCTTCCT CCGGGCTGGG AAGAGAAAGT GGATAACCTG 
GGACGGACGT ACTATGTGAA CCACAACAAC CGCACCACAC AGTGGAAGAG GCCCAGCAGT 
GTGGATGTGG TTTCAGAAAC GGAAAATGAT AATCAATTGC GGCAGATTAA CCAGGAAGCC 
CACAGAGTTT TCCGCTCTCG ACGGCACATT AGCGAAGACC TGGAAAATGA GCAGCTGGAC 
ATCAGGGACA TAGACGACTC ATGGGAGCCC ATATCAGAGG AAGATCCGAC TTTATTAACA 
GATGGTCTGA ACCATTCTCT GTCGGGACCC TCCTCTCTGG CAATGCCCCT CTCCAGCACT 
CCAGACTTCT CAGATGAGAT CAGCCTTAGA TTGTCTCTCG CTCCAGACAC CAACGGAGAA 
ATCCCGGGAC CCAGCTCTGC TGTGCAGAGT CATCTTTCCT CTCGGCTACG CTCCTCTAGT 
ATGACTGATG GAGTCAGTGA TCAAGCTCAA CCTCCCACAC CAACGCAGAG CTCCCAGACC 
CGACGAACAA GAGCACAAAC CGTCACAGGC GTTGAGGACA CCATGTCTCC TTCGGCCTCT 
TACGCCTTGA CCACGCCAGG CCTTCCACCA GGCTGGGAGG AACGCAAAGA CCCCAAGGGA 
CGAACGTACT ACGTTAATCA TAACAACCGT AGCACCACAT GGACAAGGCC CATTCTGCAG 
CAGCACACTG AGGATGGAGC CAGCACCTCG GTTGCAGCAG TAGGGGGCGC TACAGCAGCT 
ACACCCCCTG CTTCAACCCC TTCCACCTCC AGCGGCCATC TAAGCGAACC ACAAGTGCGC 
AGACCCCGTA GCCTCAGTTC CCCTACCGTC ACTCTCTCTT CCCCACTAGA GGGAGCCAAT 
AACGTCCCTA TACGGCGAGC CGTAAAGGAC ACATTCTCCA ACCCTCAGTC TCCACAGCCT 
TCGCCCTACA GCTCACCTAA AACCCAGCAT AAAGCCAACC AGAGCTTCCT GCCACCGGGA 
TGGGAAATGA GGATCGCACC CAACGGACGG CCGTTCTTCA TCGACCACAA CAGCAGAACC 
ACCACATGGG AGGATCCCCG CTTGAAGTAT CCCGTTCACA TGAGGACAAA AGCGTCTCTA 
GACCCGGGCG ACCTCGGCCC ACTTCCTAAT CTTCCAGAGG AGCCTGGGTG GGAGGAACGA 
GTGCATGCTG ATGGAAGAAC CTTTTACATC GATCACAACA ACAAAAAAAC ACAGTGGGAA 
GACCCTCGTC TCCAGAGTCC TGCCATAACT GGACCCGCGG TGCCGTATTC AAGAGAATTC 
AAGCAGAAAT ACGACTACTT CAGGAAGAAG TTAAAGAAAC CGGCAGATAT CCCCAACCGC 
TTTGAGATGA AGCTGCACAG GAATAACATC TTTGAGGAGT CCTACAGACG AATCATGTCC 
CTCAAGAGAC CTGACAGCCT GAAGGCCCGT CTGTGGATTG AGTTTGAGTC AGAGAAGGGT 
CTGGATTATG GCGGTGTGGC CCGAGAATGG TTCTTCCTGC TCTCTAAGGA GATGTTCAAC 
CCTTATTATG GGCTGTTCGA GTACTCGGCC ACAGATAACT ACACCCTTCA GATCAATCCC 
AACTCTGGCC TGTGTAATGA GGATCACCTC TCATACTTCA AGTTCATTGG TCGAGTGGCC 
GGCATGGCTG TTTATCATGG CAAACTCTTG GACGGTTTCT TCATTCGACC GTTTTATAAG 
ATGATGCTGG GCAAACAGAT CACACTGAAT GACATGGAGT CAGTGGACAG CGAATACTAC 
AACTCCCTGA AGTGGATTCT GGAAAACGAT CCCACAGAAT TAGACTTACG GTTTTGTATT 
GATGAGGACA ACTTTGGACA GACATATCAG GTAGATTTGA AGCCAAGCGG ATCAGACATG 
GTTGTAACCA ACGACAACAA GAAGGAATAC ATCGATCTGG TGATTCAGTG GAGGTTTGTG 
AACAGGGTCC AGAAACAGAT GAACGCCTTT TTGGAAGGCT TCACTGAACT CATTCCCATC 
GACTTAATAA AGATTTTTGA TGAAAACGAA CTGGAGCTGT TGATGTGTGG TCTGGGAGAT 
GTGGATGTCA ATGACTGGAG GCAACACACT GTCTATAAGA ACGGCTACTG TCCAAATCAC 
CCCGTCATCC AGTGGTTCTG GAAGGCTGTG TTGTTAATGG ATGCAGAAAA GCGCATCAGG 
CTTCTACAGT TTGTCACTGG AACATCCAGA GTGCCAATGA ACGGCTTTGC TGAACTCTAC 
GGTTCCAATG GGCCGCAGTT GTTTACTATT GAGCAGTGGG GAACTCCTGA CAAACTCCCT 
CGGGCTCACA CCTGCTTTAA TCGCTTGGAC CTCCCAATGT ATGAAACGTT TGAAGACCTG 
CGAGAGAAGC TTCTGATGGC GGTGGAAAAC GCTCAAGGCT TTGAGGGCGT CGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nedd4l ( XM_021469024.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324699.1 Danio rerio neural precursor cell expressed, developmentally down-regulated 4-like (nedd4l), transcript variant X13, mRNA.

Price & Availability↑

CloneID	ODa151023
Clone ID Related Accession (Same CDS sequence)	XM_021469024.1
Accession Version	XM_021469024.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3006bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase NEDD4-like isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTACGA ATTTTGAACC CATTTATGGA CTTTCAGAGG ATGAAAACGA ATCTCGTATT 
CTTCGCGTCA AGGTCATTGC TGGCATTGAT CTTGCGAAGA AGGACATCAT TGGTGCCAGT 
GATCCATACG TCAAACTCTC CCTGTATGTC GCTGATGAAA ACCGCGAGCT TGCCTTAGTA 
CAAACGAAAA CCATCAAAAA GACTCTTAAT CCCAAATGGA ACGAGGAATT CTATTTCAGA 
GTCTGCCCAC AAAACCACAG GCTTCTTTTC GAGGTGTTTG ACGAAAACCG CTTGACCAGA 
GATGACTTCC TGGGGCAGGT GGACGTGCCT CTTACACACT TACCGACTGA AGACCCTGCC 
ATGGAGCGTC CATACACGTT TAAAGACTTT CTCCTGCGTC CAAGAAGTCA TAAATCTCGG 
GTGAAAGGTT ACCTGAGGCT CAAGATGGCC TACCTGCCAA AGAATGGAGG ACATGAGGAG 
GAAAGCGGTG AAATGAGAGA GGAGGCTGAG GGTTGGGAAG TGACAGACTC CACAGATCCA 
GGATCTCAGA GACCTCAGCA GCTCCTGCCT CCTCTTCCTC CGGGCTGGGA AGAGAAAGTG 
GATAACCTGG GACGGACGTA CTATGTGAAC CACAACAACC GCACCACACA GTGGAAGAGG 
CCCAGCAGTG TGGATGTGGT TTCAGAAACG GAAAATGATA ATCAATTGCG GCAGATTAAC 
CAGGAAGCCC ACAGAGTTTT CCGCTCTCGA CGGCACATTA GCGAAGACCT GGAAAATGAG 
CAGCTGGACA TCAGGGACAT AGACGACTCA TGGGAGCCCA TATCAGAGGA AGATCCGACT 
TTATTAACAG ATGGTCTGAA CCATTCTCTG TCGGGACCCT CCTCTCTGGC AATGCCCCTC 
TCCAGCACTC CAGACTTCTC AGATGAGATC AGCCTTAGAT TGTCTCTCGC TCCAGACACC 
AACGGAGAAA TCCCGGGACC CAGCTCTGCT GTGCAGAGTC ATCTTTCCTC TCGGCTACGC 
TCCTCTAGTA TGACTGATGG AGTCAGTGAT CAAGCTCAAC CTCCCACACC AACGCAGAGC 
TCCCAGACCC GACGAACAAG AGCACAAACC GTCACAGGCG TTGAGGACAC CATGTCTCCT 
TCGGCCTCTT ACGCCTTGAC CACGCCAGGC CTTCCACCAG GCTGGGAGGA ACGCAAAGAC 
CCCAAGGGAC GAACGTACTA CGTTAATCAT AACAACCGTA GCACCACATG GACAAGGCCC 
ATTCTGCAGC AGCACACTGA GGATGGAGCC AGCACCTCGG TTGCAGCAGT AGGGGGCGCT 
ACAGCAGCTA CACCCCCTGC TTCAACCCCT TCCACCTCCA GCGGCCATCT AAGCGAACCA 
CAAGTGCGCA GACCCCGTAG CCTCAGTTCC CCTACCGTCA CTCTCTCTTC CCCACTAGAG 
GGAGCCAATA ACGTCCCTAT ACGGCGAGCC GTAAAGGACA CATTCTCCAA CCCTCAGTCT 
CCACAGCCTT CGCCCTACAG CTCACCTAAA ACCCAGCATA AAGCCAACCA GAGCTTCCTG 
CCACCGGGAT GGGAAATGAG GATCGCACCC AACGGACGGC CGTTCTTCAT CGACCACAAC 
AGCAGAACCA CCACATGGGA GGATCCCCGC TTGAAGTATC CCGTTCACAT GAGGACAAAA 
GCGTCTCTAG ACCCGGGCGA CCTCGGCCCA CTTCCTAATC TTCCAGAGGA GCCTGGGTGG 
GAGGAACGAG TGCATGCTGA TGGAAGAACC TTTTACATCG ATCACAACAA CAAAAAAACA 
CAGTGGGAAG ACCCTCGTCT CCAGAGTCCT GCCATAACTG GACCCGCGGT GCCGTATTCA 
AGAGAATTCA AGCAGAAATA CGACTACTTC AGGAAGAAGT TAAAGAAACC GGCAGATATC 
CCCAACCGCT TTGAGATGAA GCTGCACAGG AATAACATCT TTGAGGAGTC CTACAGACGA 
ATCATGTCCC TCAAGAGACC TGACAGCCTG AAGGCCCGTC TGTGGATTGA GTTTGAGTCA 
GAGAAGGGTC TGGATTATGG CGGTGTGGCC CGAGAATGGT TCTTCCTGCT CTCTAAGGAG 
ATGTTCAACC CTTATTATGG GCTGTTCGAG TACTCGGCCA CAGATAACTA CACCCTTCAG 
ATCAATCCCA ACTCTGGCCT GTGTAATGAG GATCACCTCT CATACTTCAA GTTCATTGGT 
CGAGTGGCCG GCATGGCTGT TTATCATGGC AAACTCTTGG ACGGTTTCTT CATTCGACCG 
TTTTATAAGA TGATGCTGGG CAAACAGATC ACACTGAATG ACATGGAGTC AGTGGACAGC 
GAATACTACA ACTCCCTGAA GTGGATTCTG GAAAACGATC CCACAGAATT AGACTTACGG 
TTTTGTATTG ATGAGGACAA CTTTGGACAG ACATATCAGG TAGATTTGAA GCCAAGCGGA 
TCAGACATGG TTGTAACCAA CGACAACAAG AAGGAATACA TCGATCTGGT GATTCAGTGG 
AGGTTTGTGA ACAGGGTCCA GAAACAGATG AACGCCTTTT TGGAAGGCTT CACTGAACTC 
ATTCCCATCG ACTTAATAAA GATTTTTGAT GAAAACGAAC TGGAGCTGTT GATGTGTGGT 
CTGGGAGATG TGGATGTCAA TGACTGGAGG CAACACACTG TCTATAAGAA CGGCTACTGT 
CCAAATCACC CCGTCATCCA GTGGTTCTGG AAGGCTGTGT TGTTAATGGA TGCAGAAAAG 
CGCATCAGGC TTCTACAGTT TGTCACTGGA ACATCCAGAG TGCCAATGAA CGGCTTTGCT 
GAACTCTACG GTTCCAATGG GCCGCAGTTG TTTACTATTG AGCAGTGGGG AACTCCTGAC 
AAACTCCCTC GGGCTCACAC CTGCTTTAAT CGCTTGGACC TCCCAATGTA TGAAACGTTT 
GAAGACCTGC GAGAGAAGCT TCTGATGGCG GTGGAAAACG CTCAAGGCTT TGAGGGCGTC 
GACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324699.1
CDS	304..3309
Translation	MATNFEPIYGLSEDENESRILRVKVIAGIDLAKKDIIGASDPYVKLSLYVADENRELALVQTKTIKKTLNPKWNEEFYFRVCPQNHRLLFEVFDENRLTRDDFLGQVDVPLTHLPTEDPAMERPYTFKDFLLRPRSHKSRVKGYLRLKMAYLPKNGGHEEESGEMREEAEGWEVTDSTDPGSQRPQQLLPPLPPGWEEKVDNLGRTYYVNHNNRTTQWKRPSSVDVVSETENDNQLRQINQEAHRVFRSRRHISEDLENEQLDIRDIDDSWEPISEEDPTLLTDGLNHSLSGPSSLAMPLSSTPDFSDEISLRLSLAPDTNGEIPGPSSAVQSHLSSRLRSSSMTDGVSDQAQPPTPTQSSQTRRTRAQTVTGVEDTMSPSASYALTTPGLPPGWEERKDPKGRTYYVNHNNRSTTWTRPILQQHTEDGASTSVAAVGGATAATPPASTPSTSSGHLSEPQVRRPRSLSSPTVTLSSPLEGANNVPIRRAVKDTFSNPQSPQPSPYSSPKTQHKANQSFLPPGWEMRIAPNGRPFFIDHNSRTTTWEDPRLKYPVHMRTKASLDPGDLGPLPNLPEEPGWEERVHADGRTFYIDHNNKKTQWEDPRLQSPAITGPAVPYSREFKQKYDYFRKKLKKPADIPNRFEMKLHRNNIFEESYRRIMSLKRPDSLKARLWIEFESEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFKFIGRVAGMAVYHGKLLDGFFIRPFYKMMLGKQITLNDMESVDSEYYNSLKWILENDPTELDLRFCIDEDNFGQTYQVDLKPSGSDMVVTNDNKKEYIDLVIQWRFVNRVQKQMNAFLEGFTELIPIDLIKIFDENELELLMCGLGDVDVNDWRQHTVYKNGYCPNHPVIQWFWKAVLLMDAEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGTPDKLPRAHTCFNRLDLPMYETFEDLREKLLMAVENAQGFEGVD

Target ORF information:

RefSeq Version	XM_021469024.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio neural precursor cell expressed, developmentally down-regulated 4-like (nedd4l), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021469024.1

ORF Insert Sequence:

ATGGCTACGA ATTTTGAACC CATTTATGGA CTTTCAGAGG ATGAAAACGA ATCTCGTATT 
CTTCGCGTCA AGGTCATTGC TGGCATTGAT CTTGCGAAGA AGGACATCAT TGGTGCCAGT 
GATCCATACG TCAAACTCTC CCTGTATGTC GCTGATGAAA ACCGCGAGCT TGCCTTAGTA 
CAAACGAAAA CCATCAAAAA GACTCTTAAT CCCAAATGGA ACGAGGAATT CTATTTCAGA 
GTCTGCCCAC AAAACCACAG GCTTCTTTTC GAGGTGTTTG ACGAAAACCG CTTGACCAGA 
GATGACTTCC TGGGGCAGGT GGACGTGCCT CTTACACACT TACCGACTGA AGACCCTGCC 
ATGGAGCGTC CATACACGTT TAAAGACTTT CTCCTGCGTC CAAGAAGTCA TAAATCTCGG 
GTGAAAGGTT ACCTGAGGCT CAAGATGGCC TACCTGCCAA AGAATGGAGG ACATGAGGAG 
GAAAGCGGTG AAATGAGAGA GGAGGCTGAG GGTTGGGAAG TGACAGACTC CACAGATCCA 
GGATCTCAGA GACCTCAGCA GCTCCTGCCT CCTCTTCCTC CGGGCTGGGA AGAGAAAGTG 
GATAACCTGG GACGGACGTA CTATGTGAAC CACAACAACC GCACCACACA GTGGAAGAGG 
CCCAGCAGTG TGGATGTGGT TTCAGAAACG GAAAATGATA ATCAATTGCG GCAGATTAAC 
CAGGAAGCCC ACAGAGTTTT CCGCTCTCGA CGGCACATTA GCGAAGACCT GGAAAATGAG 
CAGCTGGACA TCAGGGACAT AGACGACTCA TGGGAGCCCA TATCAGAGGA AGATCCGACT 
TTATTAACAG ATGGTCTGAA CCATTCTCTG TCGGGACCCT CCTCTCTGGC AATGCCCCTC 
TCCAGCACTC CAGACTTCTC AGATGAGATC AGCCTTAGAT TGTCTCTCGC TCCAGACACC 
AACGGAGAAA TCCCGGGACC CAGCTCTGCT GTGCAGAGTC ATCTTTCCTC TCGGCTACGC 
TCCTCTAGTA TGACTGATGG AGTCAGTGAT CAAGCTCAAC CTCCCACACC AACGCAGAGC 
TCCCAGACCC GACGAACAAG AGCACAAACC GTCACAGGCG TTGAGGACAC CATGTCTCCT 
TCGGCCTCTT ACGCCTTGAC CACGCCAGGC CTTCCACCAG GCTGGGAGGA ACGCAAAGAC 
CCCAAGGGAC GAACGTACTA CGTTAATCAT AACAACCGTA GCACCACATG GACAAGGCCC 
ATTCTGCAGC AGCACACTGA GGATGGAGCC AGCACCTCGG TTGCAGCAGT AGGGGGCGCT 
ACAGCAGCTA CACCCCCTGC TTCAACCCCT TCCACCTCCA GCGGCCATCT AAGCGAACCA 
CAAGTGCGCA GACCCCGTAG CCTCAGTTCC CCTACCGTCA CTCTCTCTTC CCCACTAGAG 
GGAGCCAATA ACGTCCCTAT ACGGCGAGCC GTAAAGGACA CATTCTCCAA CCCTCAGTCT 
CCACAGCCTT CGCCCTACAG CTCACCTAAA ACCCAGCATA AAGCCAACCA GAGCTTCCTG 
CCACCGGGAT GGGAAATGAG GATCGCACCC AACGGACGGC CGTTCTTCAT CGACCACAAC 
AGCAGAACCA CCACATGGGA GGATCCCCGC TTGAAGTATC CCGTTCACAT GAGGACAAAA 
GCGTCTCTAG ACCCGGGCGA CCTCGGCCCA CTTCCTAATC TTCCAGAGGA GCCTGGGTGG 
GAGGAACGAG TGCATGCTGA TGGAAGAACC TTTTACATCG ATCACAACAA CAAAAAAACA 
CAGTGGGAAG ACCCTCGTCT CCAGAGTCCT GCCATAACTG GACCCGCGGT GCCGTATTCA 
AGAGAATTCA AGCAGAAATA CGACTACTTC AGGAAGAAGT TAAAGAAACC GGCAGATATC 
CCCAACCGCT TTGAGATGAA GCTGCACAGG AATAACATCT TTGAGGAGTC CTACAGACGA 
ATCATGTCCC TCAAGAGACC TGACAGCCTG AAGGCCCGTC TGTGGATTGA GTTTGAGTCA 
GAGAAGGGTC TGGATTATGG CGGTGTGGCC CGAGAATGGT TCTTCCTGCT CTCTAAGGAG 
ATGTTCAACC CTTATTATGG GCTGTTCGAG TACTCGGCCA CAGATAACTA CACCCTTCAG 
ATCAATCCCA ACTCTGGCCT GTGTAATGAG GATCACCTCT CATACTTCAA GTTCATTGGT 
CGAGTGGCCG GCATGGCTGT TTATCATGGC AAACTCTTGG ACGGTTTCTT CATTCGACCG 
TTTTATAAGA TGATGCTGGG CAAACAGATC ACACTGAATG ACATGGAGTC AGTGGACAGC 
GAATACTACA ACTCCCTGAA GTGGATTCTG GAAAACGATC CCACAGAATT AGACTTACGG 
TTTTGTATTG ATGAGGACAA CTTTGGACAG ACATATCAGG TAGATTTGAA GCCAAGCGGA 
TCAGACATGG TTGTAACCAA CGACAACAAG AAGGAATACA TCGATCTGGT GATTCAGTGG 
AGGTTTGTGA ACAGGGTCCA GAAACAGATG AACGCCTTTT TGGAAGGCTT CACTGAACTC 
ATTCCCATCG ACTTAATAAA GATTTTTGAT GAAAACGAAC TGGAGCTGTT GATGTGTGGT 
CTGGGAGATG TGGATGTCAA TGACTGGAGG CAACACACTG TCTATAAGAA CGGCTACTGT 
CCAAATCACC CCGTCATCCA GTGGTTCTGG AAGGCTGTGT TGTTAATGGA TGCAGAAAAG 
CGCATCAGGC TTCTACAGTT TGTCACTGGA ACATCCAGAG TGCCAATGAA CGGCTTTGCT 
GAACTCTACG GTTCCAATGG GCCGCAGTTG TTTACTATTG AGCAGTGGGG AACTCCTGAC 
AAACTCCCTC GGGCTCACAC CTGCTTTAAT CGCTTGGACC TCCCAATGTA TGAAACGTTT 
GAAGACCTGC GAGAGAAGCT TCTGATGGCG GTGGAAAACG CTCAAGGCTT TGAGGGCGTC 
GACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nedd4l ( XM_021469025.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324700.1 Danio rerio neural precursor cell expressed, developmentally down-regulated 4-like (nedd4l), transcript variant X14, mRNA.

Price & Availability↑

CloneID	ODa151024
Clone ID Related Accession (Same CDS sequence)	XM_021469025.1
Accession Version	XM_021469025.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3003bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase NEDD4-like isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTACGA ATTTTGAACC CATTTATGGA CTTTCAGAGG ATGAAAACGA ATCTCGTATT 
CTTCGCGTCA AGGTCATTGC TGGCATTGAT CTTGCGAAGA AGGACATCAT TGGTGCCAGT 
GATCCATACG TCAAACTCTC CCTGTATGTC GCTGATGAAA ACCGCGAGCT TGCCTTAGTA 
CAAACGAAAA CCATCAAAAA GACTCTTAAT CCCAAATGGA ACGAGGAATT CTATTTCAGA 
GTCTGCCCAC AAAACCACAG GCTTCTTTTC GAGGTGTTTG ACGAAAACCG CTTGACCAGA 
GATGACTTCC TGGGGCAGGT GGACGTGCCT CTTACACACT TACCGACTGA AGACCCTGCC 
ATGGAGCGTC CATACACGTT TAAAGACTTT CTCCTGCGTC CAAGAAGTCA TAAATCTCGG 
GTGAAAGGTT ACCTGAGGCT CAAGATGGCC TACCTGCCAA AGAATGGAGG ACATGAGGAG 
GAAAGCGGTG AAATGAGAGA GGAGGCTGAG GGTTGGGAAG TGACAGACTC CACAGATCCA 
GGATCTCAGA GACCTCAGCA GCTCCTGCCT CCTCTTCCTC CGGGCTGGGA AGAGAAAGTG 
GATAACCTGG GACGGACGTA CTATGTGAAC CACAACAACC GCACCACACA GTGGAAGAGG 
CCCAGCAGTG TGGATGTGGT TTCAGAAACG GAAAATGATA ATCAATTGCG GCAGATTAAC 
CAGGAAGCCC ACAGAGTTTT CCGCTCTCGA CGGCACATTA GCGAAGACCT GGAAAATGAG 
CAGCTGGACA TCAGGGACAT AGACGACTCA TGGGAGCCCA TATCAGAGGA AGATCCGACT 
TTATTAACAG ATGGTCTGAA CCATTCTCTG TCGGGACCCT CCTCTCTGGC AATGCCCCTC 
TCCAGCACTC CAGACTTCTC AGATGAGATC AGCCTTAGAT TGTCTCTCGC TCCAGACACC 
AACGGAGAAA TCCCGGGACC CAGCTCTGCT GTGCAGAGTC ATCTTTCCTC TCGGCTACGC 
TCCTCTAGTA TGACTGATGG AGTCAGTGAT CAAGCTCAAC CTCCCACACC AACGAGCTCC 
CAGACCCGAC GAACAAGAGC ACAAACCGTC ACAGGCGTTG AGGACACCAT GTCTCCTTCG 
GCCTCTTACG CCTTGACCAC GCCAGGCCTT CCACCAGGCT GGGAGGAACG CAAAGACCCC 
AAGGGACGAA CGTACTACGT TAATCATAAC AACCGTAGCA CCACATGGAC AAGGCCCATT 
CTGCAGCAGC ACACTGAGGA TGGAGCCAGC ACCTCGGTTG CAGCAGTAGG GGGCGCTACA 
GCAGCTACAC CCCCTGCTTC AACCCCTTCC ACCTCCAGCG GCCATCTAAG CGAACCACAA 
GTGCGCAGAC CCCGTAGCCT CAGTTCCCCT ACCGTCACTC TCTCTTCCCC ACTAGAGGGA 
GCCAATAACG TCCCTATACG GCGAGCCGTA AAGGACACAT TCTCCAACCC TCAGTCTCCA 
CAGCCTTCGC CCTACAGCTC ACCTAAAACC CAGCATAAAG CCAACCAGAG CTTCCTGCCA 
CCGGGATGGG AAATGAGGAT CGCACCCAAC GGACGGCCGT TCTTCATCGA CCACAACAGC 
AGAACCACCA CATGGGAGGA TCCCCGCTTG AAGTATCCCG TTCACATGAG GACAAAAGCG 
TCTCTAGACC CGGGCGACCT CGGCCCACTT CCTAATCTTC CAGAGGAGCC TGGGTGGGAG 
GAACGAGTGC ATGCTGATGG AAGAACCTTT TACATCGATC ACAACAACAA AAAAACACAG 
TGGGAAGACC CTCGTCTCCA GAGTCCTGCC ATAACTGGAC CCGCGGTGCC GTATTCAAGA 
GAATTCAAGC AGAAATACGA CTACTTCAGG AAGAAGTTAA AGAAACCGGC AGATATCCCC 
AACCGCTTTG AGATGAAGCT GCACAGGAAT AACATCTTTG AGGAGTCCTA CAGACGAATC 
ATGTCCCTCA AGAGACCTGA CAGCCTGAAG GCCCGTCTGT GGATTGAGTT TGAGTCAGAG 
AAGGGTCTGG ATTATGGCGG TGTGGCCCGA GAATGGTTCT TCCTGCTCTC TAAGGAGATG 
TTCAACCCTT ATTATGGGCT GTTCGAGTAC TCGGCCACAG ATAACTACAC CCTTCAGATC 
AATCCCAACT CTGGCCTGTG TAATGAGGAT CACCTCTCAT ACTTCAAGTT CATTGGTCGA 
GTGGCCGGCA TGGCTGTTTA TCATGGCAAA CTCTTGGACG GTTTCTTCAT TCGACCGTTT 
TATAAGATGA TGCTGGGCAA ACAGATCACA CTGAATGACA TGGAGTCAGT GGACAGCGAA 
TACTACAACT CCCTGAAGTG GATTCTGGAA AACGATCCCA CAGAATTAGA CTTACGGTTT 
TGTATTGATG AGGACAACTT TGGACAGACA TATCAGGTAG ATTTGAAGCC AAGCGGATCA 
GACATGGTTG TAACCAACGA CAACAAGAAG GAATACATCG ATCTGGTGAT TCAGTGGAGG 
TTTGTGAACA GGGTCCAGAA ACAGATGAAC GCCTTTTTGG AAGGCTTCAC TGAACTCATT 
CCCATCGACT TAATAAAGAT TTTTGATGAA AACGAACTGG AGCTGTTGAT GTGTGGTCTG 
GGAGATGTGG ATGTCAATGA CTGGAGGCAA CACACTGTCT ATAAGAACGG CTACTGTCCA 
AATCACCCCG TCATCCAGTG GTTCTGGAAG GCTGTGTTGT TAATGGATGC AGAAAAGCGC 
ATCAGGCTTC TACAGTTTGT CACTGGAACA TCCAGAGTGC CAATGAACGG CTTTGCTGAA 
CTCTACGGTT CCAATGGGCC GCAGTTGTTT ACTATTGAGC AGTGGGGAAC TCCTGACAAA 
CTCCCTCGGG CTCACACCTG CTTTAATCGC TTGGACCTCC CAATGTATGA AACGTTTGAA 
GACCTGCGAG AGAAGCTTCT GATGGCGGTG GAAAACGCTC AAGGCTTTGA GGGCGTCGAC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324700.1
CDS	304..3306
Translation	MATNFEPIYGLSEDENESRILRVKVIAGIDLAKKDIIGASDPYVKLSLYVADENRELALVQTKTIKKTLNPKWNEEFYFRVCPQNHRLLFEVFDENRLTRDDFLGQVDVPLTHLPTEDPAMERPYTFKDFLLRPRSHKSRVKGYLRLKMAYLPKNGGHEEESGEMREEAEGWEVTDSTDPGSQRPQQLLPPLPPGWEEKVDNLGRTYYVNHNNRTTQWKRPSSVDVVSETENDNQLRQINQEAHRVFRSRRHISEDLENEQLDIRDIDDSWEPISEEDPTLLTDGLNHSLSGPSSLAMPLSSTPDFSDEISLRLSLAPDTNGEIPGPSSAVQSHLSSRLRSSSMTDGVSDQAQPPTPTSSQTRRTRAQTVTGVEDTMSPSASYALTTPGLPPGWEERKDPKGRTYYVNHNNRSTTWTRPILQQHTEDGASTSVAAVGGATAATPPASTPSTSSGHLSEPQVRRPRSLSSPTVTLSSPLEGANNVPIRRAVKDTFSNPQSPQPSPYSSPKTQHKANQSFLPPGWEMRIAPNGRPFFIDHNSRTTTWEDPRLKYPVHMRTKASLDPGDLGPLPNLPEEPGWEERVHADGRTFYIDHNNKKTQWEDPRLQSPAITGPAVPYSREFKQKYDYFRKKLKKPADIPNRFEMKLHRNNIFEESYRRIMSLKRPDSLKARLWIEFESEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFKFIGRVAGMAVYHGKLLDGFFIRPFYKMMLGKQITLNDMESVDSEYYNSLKWILENDPTELDLRFCIDEDNFGQTYQVDLKPSGSDMVVTNDNKKEYIDLVIQWRFVNRVQKQMNAFLEGFTELIPIDLIKIFDENELELLMCGLGDVDVNDWRQHTVYKNGYCPNHPVIQWFWKAVLLMDAEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGTPDKLPRAHTCFNRLDLPMYETFEDLREKLLMAVENAQGFEGVD

Target ORF information:

RefSeq Version	XM_021469025.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio neural precursor cell expressed, developmentally down-regulated 4-like (nedd4l), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021469025.1

ORF Insert Sequence:

ATGGCTACGA ATTTTGAACC CATTTATGGA CTTTCAGAGG ATGAAAACGA ATCTCGTATT 
CTTCGCGTCA AGGTCATTGC TGGCATTGAT CTTGCGAAGA AGGACATCAT TGGTGCCAGT 
GATCCATACG TCAAACTCTC CCTGTATGTC GCTGATGAAA ACCGCGAGCT TGCCTTAGTA 
CAAACGAAAA CCATCAAAAA GACTCTTAAT CCCAAATGGA ACGAGGAATT CTATTTCAGA 
GTCTGCCCAC AAAACCACAG GCTTCTTTTC GAGGTGTTTG ACGAAAACCG CTTGACCAGA 
GATGACTTCC TGGGGCAGGT GGACGTGCCT CTTACACACT TACCGACTGA AGACCCTGCC 
ATGGAGCGTC CATACACGTT TAAAGACTTT CTCCTGCGTC CAAGAAGTCA TAAATCTCGG 
GTGAAAGGTT ACCTGAGGCT CAAGATGGCC TACCTGCCAA AGAATGGAGG ACATGAGGAG 
GAAAGCGGTG AAATGAGAGA GGAGGCTGAG GGTTGGGAAG TGACAGACTC CACAGATCCA 
GGATCTCAGA GACCTCAGCA GCTCCTGCCT CCTCTTCCTC CGGGCTGGGA AGAGAAAGTG 
GATAACCTGG GACGGACGTA CTATGTGAAC CACAACAACC GCACCACACA GTGGAAGAGG 
CCCAGCAGTG TGGATGTGGT TTCAGAAACG GAAAATGATA ATCAATTGCG GCAGATTAAC 
CAGGAAGCCC ACAGAGTTTT CCGCTCTCGA CGGCACATTA GCGAAGACCT GGAAAATGAG 
CAGCTGGACA TCAGGGACAT AGACGACTCA TGGGAGCCCA TATCAGAGGA AGATCCGACT 
TTATTAACAG ATGGTCTGAA CCATTCTCTG TCGGGACCCT CCTCTCTGGC AATGCCCCTC 
TCCAGCACTC CAGACTTCTC AGATGAGATC AGCCTTAGAT TGTCTCTCGC TCCAGACACC 
AACGGAGAAA TCCCGGGACC CAGCTCTGCT GTGCAGAGTC ATCTTTCCTC TCGGCTACGC 
TCCTCTAGTA TGACTGATGG AGTCAGTGAT CAAGCTCAAC CTCCCACACC AACGAGCTCC 
CAGACCCGAC GAACAAGAGC ACAAACCGTC ACAGGCGTTG AGGACACCAT GTCTCCTTCG 
GCCTCTTACG CCTTGACCAC GCCAGGCCTT CCACCAGGCT GGGAGGAACG CAAAGACCCC 
AAGGGACGAA CGTACTACGT TAATCATAAC AACCGTAGCA CCACATGGAC AAGGCCCATT 
CTGCAGCAGC ACACTGAGGA TGGAGCCAGC ACCTCGGTTG CAGCAGTAGG GGGCGCTACA 
GCAGCTACAC CCCCTGCTTC AACCCCTTCC ACCTCCAGCG GCCATCTAAG CGAACCACAA 
GTGCGCAGAC CCCGTAGCCT CAGTTCCCCT ACCGTCACTC TCTCTTCCCC ACTAGAGGGA 
GCCAATAACG TCCCTATACG GCGAGCCGTA AAGGACACAT TCTCCAACCC TCAGTCTCCA 
CAGCCTTCGC CCTACAGCTC ACCTAAAACC CAGCATAAAG CCAACCAGAG CTTCCTGCCA 
CCGGGATGGG AAATGAGGAT CGCACCCAAC GGACGGCCGT TCTTCATCGA CCACAACAGC 
AGAACCACCA CATGGGAGGA TCCCCGCTTG AAGTATCCCG TTCACATGAG GACAAAAGCG 
TCTCTAGACC CGGGCGACCT CGGCCCACTT CCTAATCTTC CAGAGGAGCC TGGGTGGGAG 
GAACGAGTGC ATGCTGATGG AAGAACCTTT TACATCGATC ACAACAACAA AAAAACACAG 
TGGGAAGACC CTCGTCTCCA GAGTCCTGCC ATAACTGGAC CCGCGGTGCC GTATTCAAGA 
GAATTCAAGC AGAAATACGA CTACTTCAGG AAGAAGTTAA AGAAACCGGC AGATATCCCC 
AACCGCTTTG AGATGAAGCT GCACAGGAAT AACATCTTTG AGGAGTCCTA CAGACGAATC 
ATGTCCCTCA AGAGACCTGA CAGCCTGAAG GCCCGTCTGT GGATTGAGTT TGAGTCAGAG 
AAGGGTCTGG ATTATGGCGG TGTGGCCCGA GAATGGTTCT TCCTGCTCTC TAAGGAGATG 
TTCAACCCTT ATTATGGGCT GTTCGAGTAC TCGGCCACAG ATAACTACAC CCTTCAGATC 
AATCCCAACT CTGGCCTGTG TAATGAGGAT CACCTCTCAT ACTTCAAGTT CATTGGTCGA 
GTGGCCGGCA TGGCTGTTTA TCATGGCAAA CTCTTGGACG GTTTCTTCAT TCGACCGTTT 
TATAAGATGA TGCTGGGCAA ACAGATCACA CTGAATGACA TGGAGTCAGT GGACAGCGAA 
TACTACAACT CCCTGAAGTG GATTCTGGAA AACGATCCCA CAGAATTAGA CTTACGGTTT 
TGTATTGATG AGGACAACTT TGGACAGACA TATCAGGTAG ATTTGAAGCC AAGCGGATCA 
GACATGGTTG TAACCAACGA CAACAAGAAG GAATACATCG ATCTGGTGAT TCAGTGGAGG 
TTTGTGAACA GGGTCCAGAA ACAGATGAAC GCCTTTTTGG AAGGCTTCAC TGAACTCATT 
CCCATCGACT TAATAAAGAT TTTTGATGAA AACGAACTGG AGCTGTTGAT GTGTGGTCTG 
GGAGATGTGG ATGTCAATGA CTGGAGGCAA CACACTGTCT ATAAGAACGG CTACTGTCCA 
AATCACCCCG TCATCCAGTG GTTCTGGAAG GCTGTGTTGT TAATGGATGC AGAAAAGCGC 
ATCAGGCTTC TACAGTTTGT CACTGGAACA TCCAGAGTGC CAATGAACGG CTTTGCTGAA 
CTCTACGGTT CCAATGGGCC GCAGTTGTTT ACTATTGAGC AGTGGGGAAC TCCTGACAAA 
CTCCCTCGGG CTCACACCTG CTTTAATCGC TTGGACCTCC CAATGTATGA AACGTTTGAA 
GACCTGCGAG AGAAGCTTCT GATGGCGGTG GAAAACGCTC AAGGCTTTGA GGGCGTCGAC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nedd4l ( XM_005161083.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005161140.1 Danio rerio neural precursor cell expressed, developmentally down-regulated 4-like (nedd4l), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa25656
Clone ID Related Accession (Same CDS sequence)	XM_005161083.3
Accession Version	XM_005161083.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3774bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	E3 ubiquitin-protein ligase NEDD4-like isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005161083.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCACC GGCTTCGGTT GTATTTTGGA TCTGGTCGCA GTAACACTGC TCCAGAGTTG 
GAAGAGCTGA ATGGGACAGT GGAGGAGGAA GGTGACATAA GCTACAACAG TACAGGAGCC 
ATGGTGGTCC ATGCACAACC ACGTAACGGA GGACATGCAG AAAGTCCAGA ACCAGGCACA 
GTTGTTGGTT CGGATGCCTC TCTAAAGCGA AGCTCTTCCA TGTTCATCCC ACAGCTCCAG 
GGTCAAGCTG AGTCACGGAC AACCAAGAGC ACCTCTGTTC AAATATCTCT TCAACGCACA 
ACTGCCCCTC ATGAAGATGC ATTACCCTTT GATCCACCTC CAGCGTACCC CCAGGATCCA 
GCTCCACCAT ACACTGAACC TGGTGAAAGT TTGCCCTTAC CTCCACCTCC AACCTACTAC 
AAATCCTGCC AAAATGTGGA ACAAACTCAA AAAGATACTG AACCTAAGAG GCAAGTCTTT 
TGTGTCCGGC CATGTGATGG TCAAAATGGT TATACCCATG GAAATCCTCA AGGGATCACT 
ATTGGAGGTG TCTGTATTAG AAGAAGATCT GCAGATGGCT CGGATGTCCA GCAGTTCAGG 
ATTATGCCAT ATAGCGAGGA TGGGCACAAT GGGCACAATT GGTCTGTCCA GCAATGCTCA 
GACAGAGGTC CTGGTGCACA AAGACTAATT TTTCAGCTCC AAAAGGACCA AGGACGGCAA 
CCAAGCTCTA CCTCTCAGCC TGACGTGAGT GATTTAGGCA TCACAGGTTC TAAAGGTTCC 
CGCTTGGTGC GGTACCCCAG GATTCGACTT GAGCGGAGCA CGTCACAGCC ATTGCAGCCT 
AGAGGAGCTC CTTCACGCCT TGGATCAATG CCAAACCTTC ATGCTCCTAT CTCAGAAATG 
GAAACGGAGA GTGATTTTAT TGAGAATGGT GCTCAAGGTT GCACTTTCAA AATTAGGAAG 
GAACAGAATT CCCAACAACC GAAGTTTAAG ATTTATTTCA GCCAGGGCGG CGAGCACAGT 
CCCATCCTCG AAAGGAATAT TAGCATTGGA AAGGCGCCTA CCTGCAGCAA TCCACAGACC 
AGAGATGACT TCCTGGGGCA GGTGGACGTG CCTCTTACAC ACTTACCGAC TGAAGACCCT 
GCCATGGAGC GTCCATACAC GTTTAAAGAC TTTCTCCTGC GTCCAAGAAG TCATAAATCT 
CGGGTGAAAG GTTACCTGAG GCTCAAGATG GCCTACCTGC CAAAGAATGG AGGACATGAG 
GAGGAAAGCG GTGAAATGAG AGAGGAGGCT GAGGGTTGGG AAGTGACAGA CTCCACAGAT 
CCAGGATCTC AGAGACCTCA GCAGCTCCTG CCTCCTCTTC CTCCGGGCTG GGAAGAGAAA 
GTGGATAACC TGGGACGGAC GTACTATGTG AACCACAACA ACCGCACCAC ACAGTGGAAG 
AGGCCCAGCA GTGTGGATGT GGTTTCAGAA ACGGAAAATG ATAATCAATT GCGGCAGATT 
AACCAGGAAG CCCACAGAGT TTTCCGCTCT CGACGGCACA TTAGCGAAGA CCTGGAAAAT 
GAGCAGCTGG ACATCAGGGA CATAGACGAC TCATGGGAGC CCATATCAGA GGAAGATCCG 
ACTTTATTAA CAGATGGTCT GAACCATTCT CTGTCGGGAC CCTCCTCTCT GGCAATGCCC 
CTCTCCAGCA CTCCAGACTT CTCAGATGAG ATCAGCCTTA GATTGTCTCT CGCTCCAGAC 
ACCAACGGAG AAATCCCGGG ACCCAGCTCT GCTGTGCAGA GTCATCTTTC CTCTCGGCTA 
CGCTCCTCTA GTATGACTGA TGGAGTCAGT GATCAAGCTC AACCTCCCAC ACCAACGCAG 
AGCTCCCAGA CCCGACGAAC AAGAGCACAA ACCGTCACAG GCGTTGAGGA CACCATGTCT 
CCTTCGGCCT CTTACGCCTT GACCACGCCA GGCCTTCCAC CAGGCTGGGA GGAACGCAAA 
GACCCCAAGG GACGAACGTA CTACGTTAAT CATAACAACC GTAGCACCAC ATGGACAAGG 
CCCATTCTGC AGCAGCACAC TGAGGATGGA GCCAGCACCT CGGTTGCAGC AGTAGGGGGC 
GCTACAGCAG CTACACCCCC TGCTTCAACC CCTTCCACCT CCAGCGGCCA TCTAAGCGAA 
CCACAAGTGC GCAGACCCCG TAGCCTCAGT TCCCCTACCG TCACTCTCTC TTCCCCACTA 
GAGGGAGCCA ATAACGTCCC TATACGGCGA GCCGTAAAGG ACACATTCTC CAACCCTCAG 
TCTCCACAGC CTTCGCCCTA CAGCTCACCT AAAACCCAGC ATAAAGCCAA CCAGAGCTTC 
CTGCCACCGG GATGGGAAAT GAGGATCGCA CCCAACGGAC GGCCGTTCTT CATCGACCAC 
AACAGCAGAA CCACCACATG GGAGGATCCC CGCTTGAAGT ATCCCGTTCA CATGAGGACA 
AAAGCGTCTC TAGACCCGGG CGACCTCGGC CCACTTCCTC CTGGGTGGGA GGAACGAGTG 
CATGCTGATG GAAGAACCTT TTACATCGAT CACAACAACA AAAAAACACA GTGGGAAGAC 
CCTCGTCTCC AGAGTCCTGC CATAACTGGA CCCGCGGTGC CGTATTCAAG AGAATTCAAG 
CAGAAATACG ACTACTTCAG GAAGAAGTTA AAGAAACCGG CAGATATCCC CAACCGCTTT 
GAGATGAAGC TGCACAGGAA TAACATCTTT GAGGAGTCCT ACAGACGAAT CATGTCCCTC 
AAGAGACCTG ACAGCCTGAA GGCCCGTCTG TGGATTGAGT TTGAGTCAGA GAAGGGTCTG 
GATTATGGCG GTGTGGCCCG AGAATGGTTC TTCCTGCTCT CTAAGGAGAT GTTCAACCCT 
TATTATGGGC TGTTCGAGTA CTCGGCCACA GATAACTACA CCCTTCAGAT CAATCCCAAC 
TCTGGCCTGT GTAATGAGGA TCACCTCTCA TACTTCAAGT TCATTGGTCG AGTGGCCGGC 
ATGGCTGTTT ATCATGGCAA ACTCTTGGAC GGTTTCTTCA TTCGACCGTT TTATAAGATG 
ATGCTGGGCA AACAGATCAC ACTGAATGAC ATGGAGTCAG TGGACAGCGA ATACTACAAC 
TCCCTGAAGT GGATTCTGGA AAACGATCCC ACAGAATTAG ACTTACGGTT TTGTATTGAT 
GAGGACAACT TTGGACAGAC ATATCAGGTA GATTTGAAGC CAAGCGGATC AGACATGGTT 
GTAACCAACG ACAACAAGAA GGAATACATC GATCTGGTGA TTCAGTGGAG GTTTGTGAAC 
AGGGTCCAGA AACAGATGAA CGCCTTTTTG GAAGGCTTCA CTGAACTCAT TCCCATCGAC 
TTAATAAAGA TTTTTGATGA AAACGAACTG GAGCTGTTGA TGTGTGGTCT GGGAGATGTG 
GATGTCAATG ACTGGAGGCA ACACACTGTC TATAAGAACG GCTACTGTCC AAATCACCCC 
GTCATCCAGT GGTTCTGGAA GGCTGTGTTG TTAATGGATG CAGAAAAGCG CATCAGGCTT 
CTACAGTTTG TCACTGGAAC ATCCAGAGTG CCAATGAACG GCTTTGCTGA ACTCTACGGT 
TCCAATGGGC CGCAGTTGTT TACTATTGAG CAGTGGGGAA CTCCTGACAA ACTCCCTCGG 
GCTCACACCT GCTTTAATCG CTTGGACCTC CCAATGTATG AAACGTTTGA AGACCTGCGA 
GAGAAGCTTC TGATGGCGGT GGAAAACGCT CAAGGCTTTG AGGGCGTCGA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005161140.1
CDS	359..4132
Translation	MSHRLRLYFGSGRSNTAPELEELNGTVEEEGDISYNSTGAMVVHAQPRNGGHAESPEPGTVVGSDASLKRSSSMFIPQLQGQAESRTTKSTSVQISLQRTTAPHEDALPFDPPPAYPQDPAPPYTEPGESLPLPPPPTYYKSCQNVEQTQKDTEPKRQVFCVRPCDGQNGYTHGNPQGITIGGVCIRRRSADGSDVQQFRIMPYSEDGHNGHNWSVQQCSDRGPGAQRLIFQLQKDQGRQPSSTSQPDVSDLGITGSKGSRLVRYPRIRLERSTSQPLQPRGAPSRLGSMPNLHAPISEMETESDFIENGAQGCTFKIRKEQNSQQPKFKIYFSQGGEHSPILERNISIGKAPTCSNPQTRDDFLGQVDVPLTHLPTEDPAMERPYTFKDFLLRPRSHKSRVKGYLRLKMAYLPKNGGHEEESGEMREEAEGWEVTDSTDPGSQRPQQLLPPLPPGWEEKVDNLGRTYYVNHNNRTTQWKRPSSVDVVSETENDNQLRQINQEAHRVFRSRRHISEDLENEQLDIRDIDDSWEPISEEDPTLLTDGLNHSLSGPSSLAMPLSSTPDFSDEISLRLSLAPDTNGEIPGPSSAVQSHLSSRLRSSSMTDGVSDQAQPPTPTQSSQTRRTRAQTVTGVEDTMSPSASYALTTPGLPPGWEERKDPKGRTYYVNHNNRSTTWTRPILQQHTEDGASTSVAAVGGATAATPPASTPSTSSGHLSEPQVRRPRSLSSPTVTLSSPLEGANNVPIRRAVKDTFSNPQSPQPSPYSSPKTQHKANQSFLPPGWEMRIAPNGRPFFIDHNSRTTTWEDPRLKYPVHMRTKASLDPGDLGPLPPGWEERVHADGRTFYIDHNNKKTQWEDPRLQSPAITGPAVPYSREFKQKYDYFRKKLKKPADIPNRFEMKLHRNNIFEESYRRIMSLKRPDSLKARLWIEFESEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFKFIGRVAGMAVYHGKLLDGFFIRPFYKMMLGKQITLNDMESVDSEYYNSLKWILENDPTELDLRFCIDEDNFGQTYQVDLKPSGSDMVVTNDNKKEYIDLVIQWRFVNRVQKQMNAFLEGFTELIPIDLIKIFDENELELLMCGLGDVDVNDWRQHTVYKNGYCPNHPVIQWFWKAVLLMDAEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGTPDKLPRAHTCFNRLDLPMYETFEDLREKLLMAVENAQGFEGVD

Target ORF information:

RefSeq Version	XM_005161083.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio neural precursor cell expressed, developmentally down-regulated 4-like (nedd4l), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005161083.3

ORF Insert Sequence:

ATGTCTCACC GGCTTCGGTT GTATTTTGGA TCTGGTCGCA GTAACACTGC TCCAGAGTTG 
GAAGAGCTGA ATGGGACAGT GGAGGAGGAA GGTGACATAA GCTACAACAG TACAGGAGCC 
ATGGTGGTCC ATGCACAACC ACGTAACGGA GGACATGCAG AAAGTCCAGA ACCAGGCACA 
GTTGTTGGTT CGGATGCCTC TCTAAAGCGA AGCTCTTCCA TGTTCATCCC ACAGCTCCAG 
GGTCAAGCTG AGTCACGGAC AACCAAGAGC ACCTCTGTTC AAATATCTCT TCAACGCACA 
ACTGCCCCTC ATGAAGATGC ATTACCCTTT GATCCACCTC CAGCGTACCC CCAGGATCCA 
GCTCCACCAT ACACTGAACC TGGTGAAAGT TTGCCCTTAC CTCCACCTCC AACCTACTAC 
AAATCCTGCC AAAATGTGGA ACAAACTCAA AAAGATACTG AACCTAAGAG GCAAGTCTTT 
TGTGTCCGGC CATGTGATGG TCAAAATGGT TATACCCATG GAAATCCTCA AGGGATCACT 
ATTGGAGGTG TCTGTATTAG AAGAAGATCT GCAGATGGCT CGGATGTCCA GCAGTTCAGG 
ATTATGCCAT ATAGCGAGGA TGGGCACAAT GGGCACAATT GGTCTGTCCA GCAATGCTCA 
GACAGAGGTC CTGGTGCACA AAGACTAATT TTTCAGCTCC AAAAGGACCA AGGACGGCAA 
CCAAGCTCTA CCTCTCAGCC TGACGTGAGT GATTTAGGCA TCACAGGTTC TAAAGGTTCC 
CGCTTGGTGC GGTACCCCAG GATTCGACTT GAGCGGAGCA CGTCACAGCC ATTGCAGCCT 
AGAGGAGCTC CTTCACGCCT TGGATCAATG CCAAACCTTC ATGCTCCTAT CTCAGAAATG 
GAAACGGAGA GTGATTTTAT TGAGAATGGT GCTCAAGGTT GCACTTTCAA AATTAGGAAG 
GAACAGAATT CCCAACAACC GAAGTTTAAG ATTTATTTCA GCCAGGGCGG CGAGCACAGT 
CCCATCCTCG AAAGGAATAT TAGCATTGGA AAGGCGCCTA CCTGCAGCAA TCCACAGACC 
AGAGATGACT TCCTGGGGCA GGTGGACGTG CCTCTTACAC ACTTACCGAC TGAAGACCCT 
GCCATGGAGC GTCCATACAC GTTTAAAGAC TTTCTCCTGC GTCCAAGAAG TCATAAATCT 
CGGGTGAAAG GTTACCTGAG GCTCAAGATG GCCTACCTGC CAAAGAATGG AGGACATGAG 
GAGGAAAGCG GTGAAATGAG AGAGGAGGCT GAGGGTTGGG AAGTGACAGA CTCCACAGAT 
CCAGGATCTC AGAGACCTCA GCAGCTCCTG CCTCCTCTTC CTCCGGGCTG GGAAGAGAAA 
GTGGATAACC TGGGACGGAC GTACTATGTG AACCACAACA ACCGCACCAC ACAGTGGAAG 
AGGCCCAGCA GTGTGGATGT GGTTTCAGAA ACGGAAAATG ATAATCAATT GCGGCAGATT 
AACCAGGAAG CCCACAGAGT TTTCCGCTCT CGACGGCACA TTAGCGAAGA CCTGGAAAAT 
GAGCAGCTGG ACATCAGGGA CATAGACGAC TCATGGGAGC CCATATCAGA GGAAGATCCG 
ACTTTATTAA CAGATGGTCT GAACCATTCT CTGTCGGGAC CCTCCTCTCT GGCAATGCCC 
CTCTCCAGCA CTCCAGACTT CTCAGATGAG ATCAGCCTTA GATTGTCTCT CGCTCCAGAC 
ACCAACGGAG AAATCCCGGG ACCCAGCTCT GCTGTGCAGA GTCATCTTTC CTCTCGGCTA 
CGCTCCTCTA GTATGACTGA TGGAGTCAGT GATCAAGCTC AACCTCCCAC ACCAACGCAG 
AGCTCCCAGA CCCGACGAAC AAGAGCACAA ACCGTCACAG GCGTTGAGGA CACCATGTCT 
CCTTCGGCCT CTTACGCCTT GACCACGCCA GGCCTTCCAC CAGGCTGGGA GGAACGCAAA 
GACCCCAAGG GACGAACGTA CTACGTTAAT CATAACAACC GTAGCACCAC ATGGACAAGG 
CCCATTCTGC AGCAGCACAC TGAGGATGGA GCCAGCACCT CGGTTGCAGC AGTAGGGGGC 
GCTACAGCAG CTACACCCCC TGCTTCAACC CCTTCCACCT CCAGCGGCCA TCTAAGCGAA 
CCACAAGTGC GCAGACCCCG TAGCCTCAGT TCCCCTACCG TCACTCTCTC TTCCCCACTA 
GAGGGAGCCA ATAACGTCCC TATACGGCGA GCCGTAAAGG ACACATTCTC CAACCCTCAG 
TCTCCACAGC CTTCGCCCTA CAGCTCACCT AAAACCCAGC ATAAAGCCAA CCAGAGCTTC 
CTGCCACCGG GATGGGAAAT GAGGATCGCA CCCAACGGAC GGCCGTTCTT CATCGACCAC 
AACAGCAGAA CCACCACATG GGAGGATCCC CGCTTGAAGT ATCCCGTTCA CATGAGGACA 
AAAGCGTCTC TAGACCCGGG CGACCTCGGC CCACTTCCTC CTGGGTGGGA GGAACGAGTG 
CATGCTGATG GAAGAACCTT TTACATCGAT CACAACAACA AAAAAACACA GTGGGAAGAC 
CCTCGTCTCC AGAGTCCTGC CATAACTGGA CCCGCGGTGC CGTATTCAAG AGAATTCAAG 
CAGAAATACG ACTACTTCAG GAAGAAGTTA AAGAAACCGG CAGATATCCC CAACCGCTTT 
GAGATGAAGC TGCACAGGAA TAACATCTTT GAGGAGTCCT ACAGACGAAT CATGTCCCTC 
AAGAGACCTG ACAGCCTGAA GGCCCGTCTG TGGATTGAGT TTGAGTCAGA GAAGGGTCTG 
GATTATGGCG GTGTGGCCCG AGAATGGTTC TTCCTGCTCT CTAAGGAGAT GTTCAACCCT 
TATTATGGGC TGTTCGAGTA CTCGGCCACA GATAACTACA CCCTTCAGAT CAATCCCAAC 
TCTGGCCTGT GTAATGAGGA TCACCTCTCA TACTTCAAGT TCATTGGTCG AGTGGCCGGC 
ATGGCTGTTT ATCATGGCAA ACTCTTGGAC GGTTTCTTCA TTCGACCGTT TTATAAGATG 
ATGCTGGGCA AACAGATCAC ACTGAATGAC ATGGAGTCAG TGGACAGCGA ATACTACAAC 
TCCCTGAAGT GGATTCTGGA AAACGATCCC ACAGAATTAG ACTTACGGTT TTGTATTGAT 
GAGGACAACT TTGGACAGAC ATATCAGGTA GATTTGAAGC CAAGCGGATC AGACATGGTT 
GTAACCAACG ACAACAAGAA GGAATACATC GATCTGGTGA TTCAGTGGAG GTTTGTGAAC 
AGGGTCCAGA AACAGATGAA CGCCTTTTTG GAAGGCTTCA CTGAACTCAT TCCCATCGAC 
TTAATAAAGA TTTTTGATGA AAACGAACTG GAGCTGTTGA TGTGTGGTCT GGGAGATGTG 
GATGTCAATG ACTGGAGGCA ACACACTGTC TATAAGAACG GCTACTGTCC AAATCACCCC 
GTCATCCAGT GGTTCTGGAA GGCTGTGTTG TTAATGGATG CAGAAAAGCG CATCAGGCTT 
CTACAGTTTG TCACTGGAAC ATCCAGAGTG CCAATGAACG GCTTTGCTGA ACTCTACGGT 
TCCAATGGGC CGCAGTTGTT TACTATTGAG CAGTGGGGAA CTCCTGACAA ACTCCCTCGG 
GCTCACACCT GCTTTAATCG CTTGGACCTC CCAATGTATG AAACGTTTGA AGACCTGCGA 
GAGAAGCTTC TGATGGCGGT GGAAAACGCT CAAGGCTTTG AGGGCGTCGA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nedd4l ( XM_005161085.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005161142.1 Danio rerio neural precursor cell expressed, developmentally down-regulated 4-like (nedd4l), transcript variant X7, mRNA.

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CloneID	ODa25658
Clone ID Related Accession (Same CDS sequence)	XM_005161085.2
Accession Version	XM_005161085.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3042bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	E3 ubiquitin-protein ligase NEDD4-like isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 24, 2014 this sequence version replaced XM_005161085.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTACGA ATTTTGAACC CATTTATGGA CTTTCAGAGG ATGAAAACGA ATCTCGTATT 
CTTCGCGTCA AGGTCATTGC TGGCATTGAT CTTGCGAAGA AGGACATCAT TGGTGCCAGT 
GATCCATACG TCAAACTCTC CCTGTATGTC GCTGATGAAA ACCGCGAGCT TGCCTTAGTA 
CAAACGAAAA CCATCAAAAA GACTCTTAAT CCCAAATGGA ACGAGGAATT CTATTTCAGA 
GTCTGCCCAC AAAACCACAG GCTTCTTTTC GAGGTGTTTG ACGAAAACCG CTTGGCTCAC 
ATTGGTCTTG ATCGGCCCAA GAACGGTCAT TTCAACAATG GTCAGACCAG AGATGACTTC 
CTGGGGCAGG TGGACGTGCC TCTTACACAC TTACCGACTG AAGACCCTGC CATGGAGCGT 
CCATACACGT TTAAAGACTT TCTCCTGCGT CCAAGAAGTC ATAAATCTCG GGTGAAAGGT 
TACCTGAGGC TCAAGATGGC CTACCTGCCA AAGAATGGAG GACATGAGGA GGAAAGCGGT 
GAAATGAGAG AGGAGGCTGA GGGTTGGGAA GTGACAGACT CCACAGATCC AGGATCTCAG 
AGACCTCAGC AGCTCCTGCC TCCTCTTCCT CCGGGCTGGG AAGAGAAAGT GGATAACCTG 
GGACGGACGT ACTATGTGAA CCACAACAAC CGCACCACAC AGTGGAAGAG GCCCAGCAGT 
GTGGATGTGG TTTCAGAAAC GGAAAATGAT AATCAATTGC GGCAGATTAA CCAGGAAGCC 
CACAGAGTTT TCCGCTCTCG ACGGCACATT AGCGAAGACC TGGAAAATGA GCAGCTGGAC 
ATCAGGGACA TAGACGACTC ATGGGAGCCC ATATCAGAGG AAGATCCGAC TTTATTAACA 
GATGGTCTGA ACCATTCTCT GTCGGGACCC TCCTCTCTGG CAATGCCCCT CTCCAGCACT 
CCAGACTTCT CAGATGAGAT CAGCCTTAGA TTGTCTCTCG CTCCAGACAC CAACGGAGAA 
ATCCCGGGAC CCAGCTCTGC TGTGCAGAGT CATCTTTCCT CTCGGCTACG CTCCTCTAGT 
ATGACTGATG GAGTCAGTGA TCAAGCTCAA CCTCCCACAC CAACGCAGAG CTCCCAGACC 
CGACGAACAA GAGCACAAAC CGTCACAGGC GTTGAGGACA CCATGTCTCC TTCGGCCTCT 
TACGCCTTGA CCACGCCAGG CCTTCCACCA GGCTGGGAGG AACGCAAAGA CCCCAAGGGA 
CGAACGTACT ACGTTAATCA TAACAACCGT AGCACCACAT GGACAAGGCC CATTCTGCAG 
CAGCACACTG AGGATGGAGC CAGCACCTCG GTTGCAGCAG TAGGGGGCGC TACAGCAGCT 
ACACCCCCTG CTTCAACCCC TTCCACCTCC AGCGGCCATC TAAGCGAACC ACAAGTGCGC 
AGACCCCGTA GCCTCAGTTC CCCTACCGTC ACTCTCTCTT CCCCACTAGA GGGAGCCAAT 
AACGTCCCTA TACGGCGAGC CGTAAAGGAC ACATTCTCCA ACCCTCAGTC TCCACAGCCT 
TCGCCCTACA GCTCACCTAA AACCCAGCAT AAAGCCAACC AGAGCTTCCT GCCACCGGGA 
TGGGAAATGA GGATCGCACC CAACGGACGG CCGTTCTTCA TCGACCACAA CAGCAGAACC 
ACCACATGGG AGGATCCCCG CTTGAAGTAT CCCGTTCACA TGAGGACAAA AGCGTCTCTA 
GACCCGGGCG ACCTCGGCCC ACTTCCTCCT GGGTGGGAGG AACGAGTGCA TGCTGATGGA 
AGAACCTTTT ACATCGATCA CAACAACAAA AAAACACAGT GGGAAGACCC TCGTCTCCAG 
AGTCCTGCCA TAACTGGACC CGCGGTGCCG TATTCAAGAG AATTCAAGCA GAAATACGAC 
TACTTCAGGA AGAAGTTAAA GAAACCGGCA GATATCCCCA ACCGCTTTGA GATGAAGCTG 
CACAGGAATA ACATCTTTGA GGAGTCCTAC AGACGAATCA TGTCCCTCAA GAGACCTGAC 
AGCCTGAAGG CCCGTCTGTG GATTGAGTTT GAGTCAGAGA AGGGTCTGGA TTATGGCGGT 
GTGGCCCGAG AATGGTTCTT CCTGCTCTCT AAGGAGATGT TCAACCCTTA TTATGGGCTG 
TTCGAGTACT CGGCCACAGA TAACTACACC CTTCAGATCA ATCCCAACTC TGGCCTGTGT 
AATGAGGATC ACCTCTCATA CTTCAAGTTC ATTGGTCGAG TGGCCGGCAT GGCTGTTTAT 
CATGGCAAAC TCTTGGACGG TTTCTTCATT CGACCGTTTT ATAAGATGAT GCTGGGCAAA 
CAGATCACAC TGAATGACAT GGAGTCAGTG GACAGCGAAT ACTACAACTC CCTGAAGTGG 
ATTCTGGAAA ACGATCCCAC AGAATTAGAC TTACGGTTTT GTATTGATGA GGACAACTTT 
GGACAGACAT ATCAGGTAGA TTTGAAGCCA AGCGGATCAG ACATGGTTGT AACCAACGAC 
AACAAGAAGG AATACATCGA TCTGGTGATT CAGTGGAGGT TTGTGAACAG GGTCCAGAAA 
CAGATGAACG CCTTTTTGGA AGGCTTCACT GAACTCATTC CCATCGACTT AATAAAGATT 
TTTGATGAAA ACGAACTGGA GCTGTTGATG TGTGGTCTGG GAGATGTGGA TGTCAATGAC 
TGGAGGCAAC ACACTGTCTA TAAGAACGGC TACTGTCCAA ATCACCCCGT CATCCAGTGG 
TTCTGGAAGG CTGTGTTGTT AATGGATGCA GAAAAGCGCA TCAGGCTTCT ACAGTTTGTC 
ACTGGAACAT CCAGAGTGCC AATGAACGGC TTTGCTGAAC TCTACGGTTC CAATGGGCCG 
CAGTTGTTTA CTATTGAGCA GTGGGGAACT CCTGACAAAC TCCCTCGGGC TCACACCTGC 
TTTAATCGCT TGGACCTCCC AATGTATGAA ACGTTTGAAG ACCTGCGAGA GAAGCTTCTG 
ATGGCGGTGG AAAACGCTCA AGGCTTTGAG GGCGTCGACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005161142.1
CDS	304..3345
Translation	MATNFEPIYGLSEDENESRILRVKVIAGIDLAKKDIIGASDPYVKLSLYVADENRELALVQTKTIKKTLNPKWNEEFYFRVCPQNHRLLFEVFDENRLAHIGLDRPKNGHFNNGQTRDDFLGQVDVPLTHLPTEDPAMERPYTFKDFLLRPRSHKSRVKGYLRLKMAYLPKNGGHEEESGEMREEAEGWEVTDSTDPGSQRPQQLLPPLPPGWEEKVDNLGRTYYVNHNNRTTQWKRPSSVDVVSETENDNQLRQINQEAHRVFRSRRHISEDLENEQLDIRDIDDSWEPISEEDPTLLTDGLNHSLSGPSSLAMPLSSTPDFSDEISLRLSLAPDTNGEIPGPSSAVQSHLSSRLRSSSMTDGVSDQAQPPTPTQSSQTRRTRAQTVTGVEDTMSPSASYALTTPGLPPGWEERKDPKGRTYYVNHNNRSTTWTRPILQQHTEDGASTSVAAVGGATAATPPASTPSTSSGHLSEPQVRRPRSLSSPTVTLSSPLEGANNVPIRRAVKDTFSNPQSPQPSPYSSPKTQHKANQSFLPPGWEMRIAPNGRPFFIDHNSRTTTWEDPRLKYPVHMRTKASLDPGDLGPLPPGWEERVHADGRTFYIDHNNKKTQWEDPRLQSPAITGPAVPYSREFKQKYDYFRKKLKKPADIPNRFEMKLHRNNIFEESYRRIMSLKRPDSLKARLWIEFESEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFKFIGRVAGMAVYHGKLLDGFFIRPFYKMMLGKQITLNDMESVDSEYYNSLKWILENDPTELDLRFCIDEDNFGQTYQVDLKPSGSDMVVTNDNKKEYIDLVIQWRFVNRVQKQMNAFLEGFTELIPIDLIKIFDENELELLMCGLGDVDVNDWRQHTVYKNGYCPNHPVIQWFWKAVLLMDAEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGTPDKLPRAHTCFNRLDLPMYETFEDLREKLLMAVENAQGFEGVD

Target ORF information:

RefSeq Version	XM_005161085.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio neural precursor cell expressed, developmentally down-regulated 4-like (nedd4l), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005161085.2

ORF Insert Sequence:

ATGGCTACGA ATTTTGAACC CATTTATGGA CTTTCAGAGG ATGAAAACGA ATCTCGTATT 
CTTCGCGTCA AGGTCATTGC TGGCATTGAT CTTGCGAAGA AGGACATCAT TGGTGCCAGT 
GATCCATACG TCAAACTCTC CCTGTATGTC GCTGATGAAA ACCGCGAGCT TGCCTTAGTA 
CAAACGAAAA CCATCAAAAA GACTCTTAAT CCCAAATGGA ACGAGGAATT CTATTTCAGA 
GTCTGCCCAC AAAACCACAG GCTTCTTTTC GAGGTGTTTG ACGAAAACCG CTTGGCTCAC 
ATTGGTCTTG ATCGGCCCAA GAACGGTCAT TTCAACAATG GTCAGACCAG AGATGACTTC 
CTGGGGCAGG TGGACGTGCC TCTTACACAC TTACCGACTG AAGACCCTGC CATGGAGCGT 
CCATACACGT TTAAAGACTT TCTCCTGCGT CCAAGAAGTC ATAAATCTCG GGTGAAAGGT 
TACCTGAGGC TCAAGATGGC CTACCTGCCA AAGAATGGAG GACATGAGGA GGAAAGCGGT 
GAAATGAGAG AGGAGGCTGA GGGTTGGGAA GTGACAGACT CCACAGATCC AGGATCTCAG 
AGACCTCAGC AGCTCCTGCC TCCTCTTCCT CCGGGCTGGG AAGAGAAAGT GGATAACCTG 
GGACGGACGT ACTATGTGAA CCACAACAAC CGCACCACAC AGTGGAAGAG GCCCAGCAGT 
GTGGATGTGG TTTCAGAAAC GGAAAATGAT AATCAATTGC GGCAGATTAA CCAGGAAGCC 
CACAGAGTTT TCCGCTCTCG ACGGCACATT AGCGAAGACC TGGAAAATGA GCAGCTGGAC 
ATCAGGGACA TAGACGACTC ATGGGAGCCC ATATCAGAGG AAGATCCGAC TTTATTAACA 
GATGGTCTGA ACCATTCTCT GTCGGGACCC TCCTCTCTGG CAATGCCCCT CTCCAGCACT 
CCAGACTTCT CAGATGAGAT CAGCCTTAGA TTGTCTCTCG CTCCAGACAC CAACGGAGAA 
ATCCCGGGAC CCAGCTCTGC TGTGCAGAGT CATCTTTCCT CTCGGCTACG CTCCTCTAGT 
ATGACTGATG GAGTCAGTGA TCAAGCTCAA CCTCCCACAC CAACGCAGAG CTCCCAGACC 
CGACGAACAA GAGCACAAAC CGTCACAGGC GTTGAGGACA CCATGTCTCC TTCGGCCTCT 
TACGCCTTGA CCACGCCAGG CCTTCCACCA GGCTGGGAGG AACGCAAAGA CCCCAAGGGA 
CGAACGTACT ACGTTAATCA TAACAACCGT AGCACCACAT GGACAAGGCC CATTCTGCAG 
CAGCACACTG AGGATGGAGC CAGCACCTCG GTTGCAGCAG TAGGGGGCGC TACAGCAGCT 
ACACCCCCTG CTTCAACCCC TTCCACCTCC AGCGGCCATC TAAGCGAACC ACAAGTGCGC 
AGACCCCGTA GCCTCAGTTC CCCTACCGTC ACTCTCTCTT CCCCACTAGA GGGAGCCAAT 
AACGTCCCTA TACGGCGAGC CGTAAAGGAC ACATTCTCCA ACCCTCAGTC TCCACAGCCT 
TCGCCCTACA GCTCACCTAA AACCCAGCAT AAAGCCAACC AGAGCTTCCT GCCACCGGGA 
TGGGAAATGA GGATCGCACC CAACGGACGG CCGTTCTTCA TCGACCACAA CAGCAGAACC 
ACCACATGGG AGGATCCCCG CTTGAAGTAT CCCGTTCACA TGAGGACAAA AGCGTCTCTA 
GACCCGGGCG ACCTCGGCCC ACTTCCTCCT GGGTGGGAGG AACGAGTGCA TGCTGATGGA 
AGAACCTTTT ACATCGATCA CAACAACAAA AAAACACAGT GGGAAGACCC TCGTCTCCAG 
AGTCCTGCCA TAACTGGACC CGCGGTGCCG TATTCAAGAG AATTCAAGCA GAAATACGAC 
TACTTCAGGA AGAAGTTAAA GAAACCGGCA GATATCCCCA ACCGCTTTGA GATGAAGCTG 
CACAGGAATA ACATCTTTGA GGAGTCCTAC AGACGAATCA TGTCCCTCAA GAGACCTGAC 
AGCCTGAAGG CCCGTCTGTG GATTGAGTTT GAGTCAGAGA AGGGTCTGGA TTATGGCGGT 
GTGGCCCGAG AATGGTTCTT CCTGCTCTCT AAGGAGATGT TCAACCCTTA TTATGGGCTG 
TTCGAGTACT CGGCCACAGA TAACTACACC CTTCAGATCA ATCCCAACTC TGGCCTGTGT 
AATGAGGATC ACCTCTCATA CTTCAAGTTC ATTGGTCGAG TGGCCGGCAT GGCTGTTTAT 
CATGGCAAAC TCTTGGACGG TTTCTTCATT CGACCGTTTT ATAAGATGAT GCTGGGCAAA 
CAGATCACAC TGAATGACAT GGAGTCAGTG GACAGCGAAT ACTACAACTC CCTGAAGTGG 
ATTCTGGAAA ACGATCCCAC AGAATTAGAC TTACGGTTTT GTATTGATGA GGACAACTTT 
GGACAGACAT ATCAGGTAGA TTTGAAGCCA AGCGGATCAG ACATGGTTGT AACCAACGAC 
AACAAGAAGG AATACATCGA TCTGGTGATT CAGTGGAGGT TTGTGAACAG GGTCCAGAAA 
CAGATGAACG CCTTTTTGGA AGGCTTCACT GAACTCATTC CCATCGACTT AATAAAGATT 
TTTGATGAAA ACGAACTGGA GCTGTTGATG TGTGGTCTGG GAGATGTGGA TGTCAATGAC 
TGGAGGCAAC ACACTGTCTA TAAGAACGGC TACTGTCCAA ATCACCCCGT CATCCAGTGG 
TTCTGGAAGG CTGTGTTGTT AATGGATGCA GAAAAGCGCA TCAGGCTTCT ACAGTTTGTC 
ACTGGAACAT CCAGAGTGCC AATGAACGGC TTTGCTGAAC TCTACGGTTC CAATGGGCCG 
CAGTTGTTTA CTATTGAGCA GTGGGGAACT CCTGACAAAC TCCCTCGGGC TCACACCTGC 
TTTAATCGCT TGGACCTCCC AATGTATGAA ACGTTTGAAG ACCTGCGAGA GAAGCTTCTG 
ATGGCGGTGG AAAACGCTCA AGGCTTTGAG GGCGTCGACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nedd4l ( XM_009295227.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009293502.1 Danio rerio neural precursor cell expressed, developmentally down-regulated 4-like (nedd4l), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa43142
Clone ID Related Accession (Same CDS sequence)	XM_009295227.2
Accession Version	XM_009295227.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3771bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	E3 ubiquitin-protein ligase NEDD4-like isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009295227.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCACC GGCTTCGGTT GTATTTTGGA TCTGGTCGCA GTAACACTGC TCCAGAGTTG 
GAAGAGCTGA ATGGGACAGT GGAGGAGGAA GGTGACATAA GCTACAACAG TACAGGAGCC 
ATGGTGGTCC ATGCACAACC ACGTAACGGA GGACATGCAG AAAGTCCAGA ACCAGGCACA 
GTTGTTGGTT CGGATGCCTC TCTAAAGCGA AGCTCTTCCA TGTTCATCCC ACAGCTCCAG 
GGTCAAGCTG AGTCACGGAC AACCAAGAGC ACCTCTGTTC AAATATCTCT TCAACGCACA 
ACTGCCCCTC ATGAAGATGC ATTACCCTTT GATCCACCTC CAGCGTACCC CCAGGATCCA 
GCTCCACCAT ACACTGAACC TGGTGAAAGT TTGCCCTTAC CTCCACCTCC AACCTACTAC 
AAATCCTGCC AAAATGTGGA ACAAACTCAA AAAGATACTG AACCTAAGAG GCAAGTCTTT 
TGTGTCCGGC CATGTGATGG TCAAAATGGT TATACCCATG GAAATCCTCA AGGGATCACT 
ATTGGAGGTG TCTGTATTAG AAGAAGATCT GCAGATGGCT CGGATGTCCA GCAGTTCAGG 
ATTATGCCAT ATAGCGAGGA TGGGCACAAT GGGCACAATT GGTCTGTCCA GCAATGCTCA 
GACAGAGGTC CTGGTGCACA AAGACTAATT TTTCAGCTCC AAAAGGACCA AGGACGGCAA 
CCAAGCTCTA CCTCTCAGCC TGACGTGAGT GATTTAGGCA TCACAGGTTC TAAAGGTTCC 
CGCTTGGTGC GGTACCCCAG GATTCGACTT GAGCGGAGCA CGTCACAGCC ATTGCAGCCT 
AGAGGAGCTC CTTCACGCCT TGGATCAATG CCAAACCTTC ATGCTCCTAT CTCAGAAATG 
GAAACGGAGA GTGATTTTAT TGAGAATGGT GCTCAAGGTT GCACTTTCAA AATTAGGAAG 
GAACAGAATT CCCAACAACC GAAGTTTAAG ATTTATTTCA GCCAGGGCGG CGAGCACAGT 
CCCATCCTCG AAAGGAATAT TAGCATTGGA AAGGCGCCTA CCTGCAGCAA TCCACAGACC 
AGAGATGACT TCCTGGGGCA GGTGGACGTG CCTCTTACAC ACTTACCGAC TGAAGACCCT 
GCCATGGAGC GTCCATACAC GTTTAAAGAC TTTCTCCTGC GTCCAAGAAG TCATAAATCT 
CGGGTGAAAG GTTACCTGAG GCTCAAGATG GCCTACCTGC CAAAGAATGG AGGACATGAG 
GAGGAAAGCG GTGAAATGAG AGAGGAGGCT GAGGGTTGGG AAGTGACAGA CTCCACAGAT 
CCAGGATCTC AGAGACCTCA GCAGCTCCTG CCTCCTCTTC CTCCGGGCTG GGAAGAGAAA 
GTGGATAACC TGGGACGGAC GTACTATGTG AACCACAACA ACCGCACCAC ACAGTGGAAG 
AGGCCCAGCA GTGTGGATGT GGTTTCAGAA ACGGAAAATG ATAATCAATT GCGGCAGATT 
AACCAGGAAG CCCACAGAGT TTTCCGCTCT CGACGGCACA TTAGCGAAGA CCTGGAAAAT 
GAGCAGCTGG ACATCAGGGA CATAGACGAC TCATGGGAGC CCATATCAGA GGAAGATCCG 
ACTTTATTAA CAGATGGTCT GAACCATTCT CTGTCGGGAC CCTCCTCTCT GGCAATGCCC 
CTCTCCAGCA CTCCAGACTT CTCAGATGAG ATCAGCCTTA GATTGTCTCT CGCTCCAGAC 
ACCAACGGAG AAATCCCGGG ACCCAGCTCT GCTGTGCAGA GTCATCTTTC CTCTCGGCTA 
CGCTCCTCTA GTATGACTGA TGGAGTCAGT GATCAAGCTC AACCTCCCAC ACCAACGAGC 
TCCCAGACCC GACGAACAAG AGCACAAACC GTCACAGGCG TTGAGGACAC CATGTCTCCT 
TCGGCCTCTT ACGCCTTGAC CACGCCAGGC CTTCCACCAG GCTGGGAGGA ACGCAAAGAC 
CCCAAGGGAC GAACGTACTA CGTTAATCAT AACAACCGTA GCACCACATG GACAAGGCCC 
ATTCTGCAGC AGCACACTGA GGATGGAGCC AGCACCTCGG TTGCAGCAGT AGGGGGCGCT 
ACAGCAGCTA CACCCCCTGC TTCAACCCCT TCCACCTCCA GCGGCCATCT AAGCGAACCA 
CAAGTGCGCA GACCCCGTAG CCTCAGTTCC CCTACCGTCA CTCTCTCTTC CCCACTAGAG 
GGAGCCAATA ACGTCCCTAT ACGGCGAGCC GTAAAGGACA CATTCTCCAA CCCTCAGTCT 
CCACAGCCTT CGCCCTACAG CTCACCTAAA ACCCAGCATA AAGCCAACCA GAGCTTCCTG 
CCACCGGGAT GGGAAATGAG GATCGCACCC AACGGACGGC CGTTCTTCAT CGACCACAAC 
AGCAGAACCA CCACATGGGA GGATCCCCGC TTGAAGTATC CCGTTCACAT GAGGACAAAA 
GCGTCTCTAG ACCCGGGCGA CCTCGGCCCA CTTCCTCCTG GGTGGGAGGA ACGAGTGCAT 
GCTGATGGAA GAACCTTTTA CATCGATCAC AACAACAAAA AAACACAGTG GGAAGACCCT 
CGTCTCCAGA GTCCTGCCAT AACTGGACCC GCGGTGCCGT ATTCAAGAGA ATTCAAGCAG 
AAATACGACT ACTTCAGGAA GAAGTTAAAG AAACCGGCAG ATATCCCCAA CCGCTTTGAG 
ATGAAGCTGC ACAGGAATAA CATCTTTGAG GAGTCCTACA GACGAATCAT GTCCCTCAAG 
AGACCTGACA GCCTGAAGGC CCGTCTGTGG ATTGAGTTTG AGTCAGAGAA GGGTCTGGAT 
TATGGCGGTG TGGCCCGAGA ATGGTTCTTC CTGCTCTCTA AGGAGATGTT CAACCCTTAT 
TATGGGCTGT TCGAGTACTC GGCCACAGAT AACTACACCC TTCAGATCAA TCCCAACTCT 
GGCCTGTGTA ATGAGGATCA CCTCTCATAC TTCAAGTTCA TTGGTCGAGT GGCCGGCATG 
GCTGTTTATC ATGGCAAACT CTTGGACGGT TTCTTCATTC GACCGTTTTA TAAGATGATG 
CTGGGCAAAC AGATCACACT GAATGACATG GAGTCAGTGG ACAGCGAATA CTACAACTCC 
CTGAAGTGGA TTCTGGAAAA CGATCCCACA GAATTAGACT TACGGTTTTG TATTGATGAG 
GACAACTTTG GACAGACATA TCAGGTAGAT TTGAAGCCAA GCGGATCAGA CATGGTTGTA 
ACCAACGACA ACAAGAAGGA ATACATCGAT CTGGTGATTC AGTGGAGGTT TGTGAACAGG 
GTCCAGAAAC AGATGAACGC CTTTTTGGAA GGCTTCACTG AACTCATTCC CATCGACTTA 
ATAAAGATTT TTGATGAAAA CGAACTGGAG CTGTTGATGT GTGGTCTGGG AGATGTGGAT 
GTCAATGACT GGAGGCAACA CACTGTCTAT AAGAACGGCT ACTGTCCAAA TCACCCCGTC 
ATCCAGTGGT TCTGGAAGGC TGTGTTGTTA ATGGATGCAG AAAAGCGCAT CAGGCTTCTA 
CAGTTTGTCA CTGGAACATC CAGAGTGCCA ATGAACGGCT TTGCTGAACT CTACGGTTCC 
AATGGGCCGC AGTTGTTTAC TATTGAGCAG TGGGGAACTC CTGACAAACT CCCTCGGGCT 
CACACCTGCT TTAATCGCTT GGACCTCCCA ATGTATGAAA CGTTTGAAGA CCTGCGAGAG 
AAGCTTCTGA TGGCGGTGGA AAACGCTCAA GGCTTTGAGG GCGTCGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009293502.1
CDS	358..4128
Translation	MSHRLRLYFGSGRSNTAPELEELNGTVEEEGDISYNSTGAMVVHAQPRNGGHAESPEPGTVVGSDASLKRSSSMFIPQLQGQAESRTTKSTSVQISLQRTTAPHEDALPFDPPPAYPQDPAPPYTEPGESLPLPPPPTYYKSCQNVEQTQKDTEPKRQVFCVRPCDGQNGYTHGNPQGITIGGVCIRRRSADGSDVQQFRIMPYSEDGHNGHNWSVQQCSDRGPGAQRLIFQLQKDQGRQPSSTSQPDVSDLGITGSKGSRLVRYPRIRLERSTSQPLQPRGAPSRLGSMPNLHAPISEMETESDFIENGAQGCTFKIRKEQNSQQPKFKIYFSQGGEHSPILERNISIGKAPTCSNPQTRDDFLGQVDVPLTHLPTEDPAMERPYTFKDFLLRPRSHKSRVKGYLRLKMAYLPKNGGHEEESGEMREEAEGWEVTDSTDPGSQRPQQLLPPLPPGWEEKVDNLGRTYYVNHNNRTTQWKRPSSVDVVSETENDNQLRQINQEAHRVFRSRRHISEDLENEQLDIRDIDDSWEPISEEDPTLLTDGLNHSLSGPSSLAMPLSSTPDFSDEISLRLSLAPDTNGEIPGPSSAVQSHLSSRLRSSSMTDGVSDQAQPPTPTSSQTRRTRAQTVTGVEDTMSPSASYALTTPGLPPGWEERKDPKGRTYYVNHNNRSTTWTRPILQQHTEDGASTSVAAVGGATAATPPASTPSTSSGHLSEPQVRRPRSLSSPTVTLSSPLEGANNVPIRRAVKDTFSNPQSPQPSPYSSPKTQHKANQSFLPPGWEMRIAPNGRPFFIDHNSRTTTWEDPRLKYPVHMRTKASLDPGDLGPLPPGWEERVHADGRTFYIDHNNKKTQWEDPRLQSPAITGPAVPYSREFKQKYDYFRKKLKKPADIPNRFEMKLHRNNIFEESYRRIMSLKRPDSLKARLWIEFESEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFKFIGRVAGMAVYHGKLLDGFFIRPFYKMMLGKQITLNDMESVDSEYYNSLKWILENDPTELDLRFCIDEDNFGQTYQVDLKPSGSDMVVTNDNKKEYIDLVIQWRFVNRVQKQMNAFLEGFTELIPIDLIKIFDENELELLMCGLGDVDVNDWRQHTVYKNGYCPNHPVIQWFWKAVLLMDAEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGTPDKLPRAHTCFNRLDLPMYETFEDLREKLLMAVENAQGFEGVD

Target ORF information:

RefSeq Version	XM_009295227.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio neural precursor cell expressed, developmentally down-regulated 4-like (nedd4l), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009295227.2

ORF Insert Sequence:

ATGTCTCACC GGCTTCGGTT GTATTTTGGA TCTGGTCGCA GTAACACTGC TCCAGAGTTG 
GAAGAGCTGA ATGGGACAGT GGAGGAGGAA GGTGACATAA GCTACAACAG TACAGGAGCC 
ATGGTGGTCC ATGCACAACC ACGTAACGGA GGACATGCAG AAAGTCCAGA ACCAGGCACA 
GTTGTTGGTT CGGATGCCTC TCTAAAGCGA AGCTCTTCCA TGTTCATCCC ACAGCTCCAG 
GGTCAAGCTG AGTCACGGAC AACCAAGAGC ACCTCTGTTC AAATATCTCT TCAACGCACA 
ACTGCCCCTC ATGAAGATGC ATTACCCTTT GATCCACCTC CAGCGTACCC CCAGGATCCA 
GCTCCACCAT ACACTGAACC TGGTGAAAGT TTGCCCTTAC CTCCACCTCC AACCTACTAC 
AAATCCTGCC AAAATGTGGA ACAAACTCAA AAAGATACTG AACCTAAGAG GCAAGTCTTT 
TGTGTCCGGC CATGTGATGG TCAAAATGGT TATACCCATG GAAATCCTCA AGGGATCACT 
ATTGGAGGTG TCTGTATTAG AAGAAGATCT GCAGATGGCT CGGATGTCCA GCAGTTCAGG 
ATTATGCCAT ATAGCGAGGA TGGGCACAAT GGGCACAATT GGTCTGTCCA GCAATGCTCA 
GACAGAGGTC CTGGTGCACA AAGACTAATT TTTCAGCTCC AAAAGGACCA AGGACGGCAA 
CCAAGCTCTA CCTCTCAGCC TGACGTGAGT GATTTAGGCA TCACAGGTTC TAAAGGTTCC 
CGCTTGGTGC GGTACCCCAG GATTCGACTT GAGCGGAGCA CGTCACAGCC ATTGCAGCCT 
AGAGGAGCTC CTTCACGCCT TGGATCAATG CCAAACCTTC ATGCTCCTAT CTCAGAAATG 
GAAACGGAGA GTGATTTTAT TGAGAATGGT GCTCAAGGTT GCACTTTCAA AATTAGGAAG 
GAACAGAATT CCCAACAACC GAAGTTTAAG ATTTATTTCA GCCAGGGCGG CGAGCACAGT 
CCCATCCTCG AAAGGAATAT TAGCATTGGA AAGGCGCCTA CCTGCAGCAA TCCACAGACC 
AGAGATGACT TCCTGGGGCA GGTGGACGTG CCTCTTACAC ACTTACCGAC TGAAGACCCT 
GCCATGGAGC GTCCATACAC GTTTAAAGAC TTTCTCCTGC GTCCAAGAAG TCATAAATCT 
CGGGTGAAAG GTTACCTGAG GCTCAAGATG GCCTACCTGC CAAAGAATGG AGGACATGAG 
GAGGAAAGCG GTGAAATGAG AGAGGAGGCT GAGGGTTGGG AAGTGACAGA CTCCACAGAT 
CCAGGATCTC AGAGACCTCA GCAGCTCCTG CCTCCTCTTC CTCCGGGCTG GGAAGAGAAA 
GTGGATAACC TGGGACGGAC GTACTATGTG AACCACAACA ACCGCACCAC ACAGTGGAAG 
AGGCCCAGCA GTGTGGATGT GGTTTCAGAA ACGGAAAATG ATAATCAATT GCGGCAGATT 
AACCAGGAAG CCCACAGAGT TTTCCGCTCT CGACGGCACA TTAGCGAAGA CCTGGAAAAT 
GAGCAGCTGG ACATCAGGGA CATAGACGAC TCATGGGAGC CCATATCAGA GGAAGATCCG 
ACTTTATTAA CAGATGGTCT GAACCATTCT CTGTCGGGAC CCTCCTCTCT GGCAATGCCC 
CTCTCCAGCA CTCCAGACTT CTCAGATGAG ATCAGCCTTA GATTGTCTCT CGCTCCAGAC 
ACCAACGGAG AAATCCCGGG ACCCAGCTCT GCTGTGCAGA GTCATCTTTC CTCTCGGCTA 
CGCTCCTCTA GTATGACTGA TGGAGTCAGT GATCAAGCTC AACCTCCCAC ACCAACGAGC 
TCCCAGACCC GACGAACAAG AGCACAAACC GTCACAGGCG TTGAGGACAC CATGTCTCCT 
TCGGCCTCTT ACGCCTTGAC CACGCCAGGC CTTCCACCAG GCTGGGAGGA ACGCAAAGAC 
CCCAAGGGAC GAACGTACTA CGTTAATCAT AACAACCGTA GCACCACATG GACAAGGCCC 
ATTCTGCAGC AGCACACTGA GGATGGAGCC AGCACCTCGG TTGCAGCAGT AGGGGGCGCT 
ACAGCAGCTA CACCCCCTGC TTCAACCCCT TCCACCTCCA GCGGCCATCT AAGCGAACCA 
CAAGTGCGCA GACCCCGTAG CCTCAGTTCC CCTACCGTCA CTCTCTCTTC CCCACTAGAG 
GGAGCCAATA ACGTCCCTAT ACGGCGAGCC GTAAAGGACA CATTCTCCAA CCCTCAGTCT 
CCACAGCCTT CGCCCTACAG CTCACCTAAA ACCCAGCATA AAGCCAACCA GAGCTTCCTG 
CCACCGGGAT GGGAAATGAG GATCGCACCC AACGGACGGC CGTTCTTCAT CGACCACAAC 
AGCAGAACCA CCACATGGGA GGATCCCCGC TTGAAGTATC CCGTTCACAT GAGGACAAAA 
GCGTCTCTAG ACCCGGGCGA CCTCGGCCCA CTTCCTCCTG GGTGGGAGGA ACGAGTGCAT 
GCTGATGGAA GAACCTTTTA CATCGATCAC AACAACAAAA AAACACAGTG GGAAGACCCT 
CGTCTCCAGA GTCCTGCCAT AACTGGACCC GCGGTGCCGT ATTCAAGAGA ATTCAAGCAG 
AAATACGACT ACTTCAGGAA GAAGTTAAAG AAACCGGCAG ATATCCCCAA CCGCTTTGAG 
ATGAAGCTGC ACAGGAATAA CATCTTTGAG GAGTCCTACA GACGAATCAT GTCCCTCAAG 
AGACCTGACA GCCTGAAGGC CCGTCTGTGG ATTGAGTTTG AGTCAGAGAA GGGTCTGGAT 
TATGGCGGTG TGGCCCGAGA ATGGTTCTTC CTGCTCTCTA AGGAGATGTT CAACCCTTAT 
TATGGGCTGT TCGAGTACTC GGCCACAGAT AACTACACCC TTCAGATCAA TCCCAACTCT 
GGCCTGTGTA ATGAGGATCA CCTCTCATAC TTCAAGTTCA TTGGTCGAGT GGCCGGCATG 
GCTGTTTATC ATGGCAAACT CTTGGACGGT TTCTTCATTC GACCGTTTTA TAAGATGATG 
CTGGGCAAAC AGATCACACT GAATGACATG GAGTCAGTGG ACAGCGAATA CTACAACTCC 
CTGAAGTGGA TTCTGGAAAA CGATCCCACA GAATTAGACT TACGGTTTTG TATTGATGAG 
GACAACTTTG GACAGACATA TCAGGTAGAT TTGAAGCCAA GCGGATCAGA CATGGTTGTA 
ACCAACGACA ACAAGAAGGA ATACATCGAT CTGGTGATTC AGTGGAGGTT TGTGAACAGG 
GTCCAGAAAC AGATGAACGC CTTTTTGGAA GGCTTCACTG AACTCATTCC CATCGACTTA 
ATAAAGATTT TTGATGAAAA CGAACTGGAG CTGTTGATGT GTGGTCTGGG AGATGTGGAT 
GTCAATGACT GGAGGCAACA CACTGTCTAT AAGAACGGCT ACTGTCCAAA TCACCCCGTC 
ATCCAGTGGT TCTGGAAGGC TGTGTTGTTA ATGGATGCAG AAAAGCGCAT CAGGCTTCTA 
CAGTTTGTCA CTGGAACATC CAGAGTGCCA ATGAACGGCT TTGCTGAACT CTACGGTTCC 
AATGGGCCGC AGTTGTTTAC TATTGAGCAG TGGGGAACTC CTGACAAACT CCCTCGGGCT 
CACACCTGCT TTAATCGCTT GGACCTCCCA ATGTATGAAA CGTTTGAAGA CCTGCGAGAG 
AAGCTTCTGA TGGCGGTGGA AAACGCTCAA GGCTTTGAGG GCGTCGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nedd4l ( XM_009295228.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009293503.1 Danio rerio neural precursor cell expressed, developmentally down-regulated 4-like (nedd4l), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa43143
Clone ID Related Accession (Same CDS sequence)	XM_009295228.2
Accession Version	XM_009295228.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3771bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	E3 ubiquitin-protein ligase NEDD4-like isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009295228.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCACC GGCTTCGGTT GTATTTTGGA TCTGGTCGCA GTAACACTGC TCCAGAGTTG 
GAAGAGCTGA ATGGGACAGT GGAGGAGGAA GGTGACATAA GCTACAACAG TACAGGAGCC 
ATGGTGGTCC ATGCACAACC ACGTAACGGA GGACATGCAG AAAGTCCAGA ACCAGGCACA 
GTTGTTGGTT CGGATGCCTC TCTAAAGCGA AGCTCTTCCA TGTTCATCCC ACAGCTCCAG 
GGTCAAGCTG AGTCACGGAC AACCAAGAGC ACCTCTGTTC AAATATCTCT TCAACGCACA 
ACTGCCCCTC ATGAAGATGC ATTACCCTTT GATCCACCTC CAGCGTACCC CCAGGATCCA 
GCTCCACCAT ACACTGAACC TGGTGAAAGT TTGCCCTTAC CTCCACCTCC AACCTACTAC 
AAATCCTGCC AAAATGTGGA ACAAACTCAA AAAGATACTG AACCTAAGAG GCAAGTCTTT 
TGTGTCCGGC CATGTGATGG TCAAAATGGT TATACCCATG GAAATCCTCA AGGGATCACT 
ATTGGAGGTG TCTGTATTAG AAGAAGATCT GCAGATGGCT CGGATGTCCA GCAGTTCAGG 
ATTATGCCAT ATAGCGAGGA TGGGCACAAT GGGCACAATT GGTCTGTCCA GCAATGCTCA 
GACAGAGGTC CTGGTGCACA AAGACTAATT TTTCAGCTCC AAAAGGACCA AGGACGGCAA 
CCAAGCTCTA CCTCTCAGCC TGACGTGAGT GATTTAGGCA TCACAGGTTC TAAAGGTTCC 
CGCTTGGTGC GGTACCCCAG GATTCGACTT GAGCGGAGCA CGTCACAGCC ATTGCAGCCT 
AGAGGAGCTC CTTCACGCCT TGGATCAATG CCAAACCTTC ATGCTCCTAT CTCAGAAATG 
GAAACGGAGA GTGATTTTAT TGAGAATGGT GCTCAAGGTT GCACTTTCAA AATTAGGAAG 
GAACAGAATT CCCAACAACC GAAGTTTAAG ATTTATTTCA GCCAGGGCGG CGAGCACAGT 
CCCATCCTCG AAAGGAATAT TAGCATTGGA AAGGCGCCTA CCTGCAGCAA TCCACAGACC 
AGAGATGACT TCCTGGGGCA GGTGGACGTG CCTCTTACAC ACTTACCGAC TGAAGACCCT 
GCCATGGAGC GTCCATACAC GTTTAAAGAC TTTCTCCTGC GTCCAAGAAG TCATAAATCT 
CGGGTGAAAG GTTACCTGAG GCTCAAGATG GCCTACCTGC CAAAGAATGG AGGACATGAG 
GAGGAAAGCG GTGAAATGAG AGAGGAGGCT GAGGGTTGGG AAGTGACAGA CTCCACAGAT 
CCAGGATCTC AGAGACCTCA GCAGCTCCTG CCTCCTCTTC CTCCGGGCTG GGAAGAGAAA 
GTGGATAACC TGGGACGGAC GTACTATGTG AACCACAACA ACCGCACCAC ACAGTGGAAG 
AGGCCCAGCA GTGTGGATGT GGTTTCAGAA ACGGAAAATG ATAATCAATT GCGGCAGATT 
AACCAGGAAG CCCACAGAGT TTTCCGCTCT CGACGGCACA TTAGCGAAGA CCTGGAAAAT 
GAGCAGCTGG ACATCAGGGA CATAGACGAC TCATGGGAGC CCATATCAGA GGAAGATCCG 
ACTTTATTAA CAGATGGTCT GAACCATTCT CTGTCGGGAC CCTCCTCTCT GGCAATGCCC 
CTCTCCAGCA CTCCAGACTT CTCAGATGAG ATCAGCCTTA GATTGTCTCT CGCTCCAGAC 
ACCAACGGAG AAATCCCGGG ACCCAGCTCT GCTGTGCAGA GTCATCTTTC CTCTCGGCTA 
CGCTCCTCTA GTATGACTGA TGGAGTCAGT GATCAAGCTC AACCTCCCAC ACCAACGCAG 
AGCTCCCAGA CCCGACGAAC AAGAGCACAA ACCGTCACAG GCGTTGAGGA CACCATGTCT 
CCTTCGGCCT CTTACGCCTT GACCACGCCA GGCCTTCCAC CAGGCTGGGA GGAACGCAAA 
GACCCCAAGG GACGAACGTA CTACGTTAAT CATAACAACC GTAGCACCAC ATGGACAAGG 
CCCATTCTGC AGCACACTGA GGATGGAGCC AGCACCTCGG TTGCAGCAGT AGGGGGCGCT 
ACAGCAGCTA CACCCCCTGC TTCAACCCCT TCCACCTCCA GCGGCCATCT AAGCGAACCA 
CAAGTGCGCA GACCCCGTAG CCTCAGTTCC CCTACCGTCA CTCTCTCTTC CCCACTAGAG 
GGAGCCAATA ACGTCCCTAT ACGGCGAGCC GTAAAGGACA CATTCTCCAA CCCTCAGTCT 
CCACAGCCTT CGCCCTACAG CTCACCTAAA ACCCAGCATA AAGCCAACCA GAGCTTCCTG 
CCACCGGGAT GGGAAATGAG GATCGCACCC AACGGACGGC CGTTCTTCAT CGACCACAAC 
AGCAGAACCA CCACATGGGA GGATCCCCGC TTGAAGTATC CCGTTCACAT GAGGACAAAA 
GCGTCTCTAG ACCCGGGCGA CCTCGGCCCA CTTCCTCCTG GGTGGGAGGA ACGAGTGCAT 
GCTGATGGAA GAACCTTTTA CATCGATCAC AACAACAAAA AAACACAGTG GGAAGACCCT 
CGTCTCCAGA GTCCTGCCAT AACTGGACCC GCGGTGCCGT ATTCAAGAGA ATTCAAGCAG 
AAATACGACT ACTTCAGGAA GAAGTTAAAG AAACCGGCAG ATATCCCCAA CCGCTTTGAG 
ATGAAGCTGC ACAGGAATAA CATCTTTGAG GAGTCCTACA GACGAATCAT GTCCCTCAAG 
AGACCTGACA GCCTGAAGGC CCGTCTGTGG ATTGAGTTTG AGTCAGAGAA GGGTCTGGAT 
TATGGCGGTG TGGCCCGAGA ATGGTTCTTC CTGCTCTCTA AGGAGATGTT CAACCCTTAT 
TATGGGCTGT TCGAGTACTC GGCCACAGAT AACTACACCC TTCAGATCAA TCCCAACTCT 
GGCCTGTGTA ATGAGGATCA CCTCTCATAC TTCAAGTTCA TTGGTCGAGT GGCCGGCATG 
GCTGTTTATC ATGGCAAACT CTTGGACGGT TTCTTCATTC GACCGTTTTA TAAGATGATG 
CTGGGCAAAC AGATCACACT GAATGACATG GAGTCAGTGG ACAGCGAATA CTACAACTCC 
CTGAAGTGGA TTCTGGAAAA CGATCCCACA GAATTAGACT TACGGTTTTG TATTGATGAG 
GACAACTTTG GACAGACATA TCAGGTAGAT TTGAAGCCAA GCGGATCAGA CATGGTTGTA 
ACCAACGACA ACAAGAAGGA ATACATCGAT CTGGTGATTC AGTGGAGGTT TGTGAACAGG 
GTCCAGAAAC AGATGAACGC CTTTTTGGAA GGCTTCACTG AACTCATTCC CATCGACTTA 
ATAAAGATTT TTGATGAAAA CGAACTGGAG CTGTTGATGT GTGGTCTGGG AGATGTGGAT 
GTCAATGACT GGAGGCAACA CACTGTCTAT AAGAACGGCT ACTGTCCAAA TCACCCCGTC 
ATCCAGTGGT TCTGGAAGGC TGTGTTGTTA ATGGATGCAG AAAAGCGCAT CAGGCTTCTA 
CAGTTTGTCA CTGGAACATC CAGAGTGCCA ATGAACGGCT TTGCTGAACT CTACGGTTCC 
AATGGGCCGC AGTTGTTTAC TATTGAGCAG TGGGGAACTC CTGACAAACT CCCTCGGGCT 
CACACCTGCT TTAATCGCTT GGACCTCCCA ATGTATGAAA CGTTTGAAGA CCTGCGAGAG 
AAGCTTCTGA TGGCGGTGGA AAACGCTCAA GGCTTTGAGG GCGTCGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009293503.1
CDS	359..4129
Translation	MSHRLRLYFGSGRSNTAPELEELNGTVEEEGDISYNSTGAMVVHAQPRNGGHAESPEPGTVVGSDASLKRSSSMFIPQLQGQAESRTTKSTSVQISLQRTTAPHEDALPFDPPPAYPQDPAPPYTEPGESLPLPPPPTYYKSCQNVEQTQKDTEPKRQVFCVRPCDGQNGYTHGNPQGITIGGVCIRRRSADGSDVQQFRIMPYSEDGHNGHNWSVQQCSDRGPGAQRLIFQLQKDQGRQPSSTSQPDVSDLGITGSKGSRLVRYPRIRLERSTSQPLQPRGAPSRLGSMPNLHAPISEMETESDFIENGAQGCTFKIRKEQNSQQPKFKIYFSQGGEHSPILERNISIGKAPTCSNPQTRDDFLGQVDVPLTHLPTEDPAMERPYTFKDFLLRPRSHKSRVKGYLRLKMAYLPKNGGHEEESGEMREEAEGWEVTDSTDPGSQRPQQLLPPLPPGWEEKVDNLGRTYYVNHNNRTTQWKRPSSVDVVSETENDNQLRQINQEAHRVFRSRRHISEDLENEQLDIRDIDDSWEPISEEDPTLLTDGLNHSLSGPSSLAMPLSSTPDFSDEISLRLSLAPDTNGEIPGPSSAVQSHLSSRLRSSSMTDGVSDQAQPPTPTQSSQTRRTRAQTVTGVEDTMSPSASYALTTPGLPPGWEERKDPKGRTYYVNHNNRSTTWTRPILQHTEDGASTSVAAVGGATAATPPASTPSTSSGHLSEPQVRRPRSLSSPTVTLSSPLEGANNVPIRRAVKDTFSNPQSPQPSPYSSPKTQHKANQSFLPPGWEMRIAPNGRPFFIDHNSRTTTWEDPRLKYPVHMRTKASLDPGDLGPLPPGWEERVHADGRTFYIDHNNKKTQWEDPRLQSPAITGPAVPYSREFKQKYDYFRKKLKKPADIPNRFEMKLHRNNIFEESYRRIMSLKRPDSLKARLWIEFESEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFKFIGRVAGMAVYHGKLLDGFFIRPFYKMMLGKQITLNDMESVDSEYYNSLKWILENDPTELDLRFCIDEDNFGQTYQVDLKPSGSDMVVTNDNKKEYIDLVIQWRFVNRVQKQMNAFLEGFTELIPIDLIKIFDENELELLMCGLGDVDVNDWRQHTVYKNGYCPNHPVIQWFWKAVLLMDAEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGTPDKLPRAHTCFNRLDLPMYETFEDLREKLLMAVENAQGFEGVD

Target ORF information:

RefSeq Version	XM_009295228.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio neural precursor cell expressed, developmentally down-regulated 4-like (nedd4l), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009295228.2

ORF Insert Sequence:

ATGTCTCACC GGCTTCGGTT GTATTTTGGA TCTGGTCGCA GTAACACTGC TCCAGAGTTG 
GAAGAGCTGA ATGGGACAGT GGAGGAGGAA GGTGACATAA GCTACAACAG TACAGGAGCC 
ATGGTGGTCC ATGCACAACC ACGTAACGGA GGACATGCAG AAAGTCCAGA ACCAGGCACA 
GTTGTTGGTT CGGATGCCTC TCTAAAGCGA AGCTCTTCCA TGTTCATCCC ACAGCTCCAG 
GGTCAAGCTG AGTCACGGAC AACCAAGAGC ACCTCTGTTC AAATATCTCT TCAACGCACA 
ACTGCCCCTC ATGAAGATGC ATTACCCTTT GATCCACCTC CAGCGTACCC CCAGGATCCA 
GCTCCACCAT ACACTGAACC TGGTGAAAGT TTGCCCTTAC CTCCACCTCC AACCTACTAC 
AAATCCTGCC AAAATGTGGA ACAAACTCAA AAAGATACTG AACCTAAGAG GCAAGTCTTT 
TGTGTCCGGC CATGTGATGG TCAAAATGGT TATACCCATG GAAATCCTCA AGGGATCACT 
ATTGGAGGTG TCTGTATTAG AAGAAGATCT GCAGATGGCT CGGATGTCCA GCAGTTCAGG 
ATTATGCCAT ATAGCGAGGA TGGGCACAAT GGGCACAATT GGTCTGTCCA GCAATGCTCA 
GACAGAGGTC CTGGTGCACA AAGACTAATT TTTCAGCTCC AAAAGGACCA AGGACGGCAA 
CCAAGCTCTA CCTCTCAGCC TGACGTGAGT GATTTAGGCA TCACAGGTTC TAAAGGTTCC 
CGCTTGGTGC GGTACCCCAG GATTCGACTT GAGCGGAGCA CGTCACAGCC ATTGCAGCCT 
AGAGGAGCTC CTTCACGCCT TGGATCAATG CCAAACCTTC ATGCTCCTAT CTCAGAAATG 
GAAACGGAGA GTGATTTTAT TGAGAATGGT GCTCAAGGTT GCACTTTCAA AATTAGGAAG 
GAACAGAATT CCCAACAACC GAAGTTTAAG ATTTATTTCA GCCAGGGCGG CGAGCACAGT 
CCCATCCTCG AAAGGAATAT TAGCATTGGA AAGGCGCCTA CCTGCAGCAA TCCACAGACC 
AGAGATGACT TCCTGGGGCA GGTGGACGTG CCTCTTACAC ACTTACCGAC TGAAGACCCT 
GCCATGGAGC GTCCATACAC GTTTAAAGAC TTTCTCCTGC GTCCAAGAAG TCATAAATCT 
CGGGTGAAAG GTTACCTGAG GCTCAAGATG GCCTACCTGC CAAAGAATGG AGGACATGAG 
GAGGAAAGCG GTGAAATGAG AGAGGAGGCT GAGGGTTGGG AAGTGACAGA CTCCACAGAT 
CCAGGATCTC AGAGACCTCA GCAGCTCCTG CCTCCTCTTC CTCCGGGCTG GGAAGAGAAA 
GTGGATAACC TGGGACGGAC GTACTATGTG AACCACAACA ACCGCACCAC ACAGTGGAAG 
AGGCCCAGCA GTGTGGATGT GGTTTCAGAA ACGGAAAATG ATAATCAATT GCGGCAGATT 
AACCAGGAAG CCCACAGAGT TTTCCGCTCT CGACGGCACA TTAGCGAAGA CCTGGAAAAT 
GAGCAGCTGG ACATCAGGGA CATAGACGAC TCATGGGAGC CCATATCAGA GGAAGATCCG 
ACTTTATTAA CAGATGGTCT GAACCATTCT CTGTCGGGAC CCTCCTCTCT GGCAATGCCC 
CTCTCCAGCA CTCCAGACTT CTCAGATGAG ATCAGCCTTA GATTGTCTCT CGCTCCAGAC 
ACCAACGGAG AAATCCCGGG ACCCAGCTCT GCTGTGCAGA GTCATCTTTC CTCTCGGCTA 
CGCTCCTCTA GTATGACTGA TGGAGTCAGT GATCAAGCTC AACCTCCCAC ACCAACGCAG 
AGCTCCCAGA CCCGACGAAC AAGAGCACAA ACCGTCACAG GCGTTGAGGA CACCATGTCT 
CCTTCGGCCT CTTACGCCTT GACCACGCCA GGCCTTCCAC CAGGCTGGGA GGAACGCAAA 
GACCCCAAGG GACGAACGTA CTACGTTAAT CATAACAACC GTAGCACCAC ATGGACAAGG 
CCCATTCTGC AGCACACTGA GGATGGAGCC AGCACCTCGG TTGCAGCAGT AGGGGGCGCT 
ACAGCAGCTA CACCCCCTGC TTCAACCCCT TCCACCTCCA GCGGCCATCT AAGCGAACCA 
CAAGTGCGCA GACCCCGTAG CCTCAGTTCC CCTACCGTCA CTCTCTCTTC CCCACTAGAG 
GGAGCCAATA ACGTCCCTAT ACGGCGAGCC GTAAAGGACA CATTCTCCAA CCCTCAGTCT 
CCACAGCCTT CGCCCTACAG CTCACCTAAA ACCCAGCATA AAGCCAACCA GAGCTTCCTG 
CCACCGGGAT GGGAAATGAG GATCGCACCC AACGGACGGC CGTTCTTCAT CGACCACAAC 
AGCAGAACCA CCACATGGGA GGATCCCCGC TTGAAGTATC CCGTTCACAT GAGGACAAAA 
GCGTCTCTAG ACCCGGGCGA CCTCGGCCCA CTTCCTCCTG GGTGGGAGGA ACGAGTGCAT 
GCTGATGGAA GAACCTTTTA CATCGATCAC AACAACAAAA AAACACAGTG GGAAGACCCT 
CGTCTCCAGA GTCCTGCCAT AACTGGACCC GCGGTGCCGT ATTCAAGAGA ATTCAAGCAG 
AAATACGACT ACTTCAGGAA GAAGTTAAAG AAACCGGCAG ATATCCCCAA CCGCTTTGAG 
ATGAAGCTGC ACAGGAATAA CATCTTTGAG GAGTCCTACA GACGAATCAT GTCCCTCAAG 
AGACCTGACA GCCTGAAGGC CCGTCTGTGG ATTGAGTTTG AGTCAGAGAA GGGTCTGGAT 
TATGGCGGTG TGGCCCGAGA ATGGTTCTTC CTGCTCTCTA AGGAGATGTT CAACCCTTAT 
TATGGGCTGT TCGAGTACTC GGCCACAGAT AACTACACCC TTCAGATCAA TCCCAACTCT 
GGCCTGTGTA ATGAGGATCA CCTCTCATAC TTCAAGTTCA TTGGTCGAGT GGCCGGCATG 
GCTGTTTATC ATGGCAAACT CTTGGACGGT TTCTTCATTC GACCGTTTTA TAAGATGATG 
CTGGGCAAAC AGATCACACT GAATGACATG GAGTCAGTGG ACAGCGAATA CTACAACTCC 
CTGAAGTGGA TTCTGGAAAA CGATCCCACA GAATTAGACT TACGGTTTTG TATTGATGAG 
GACAACTTTG GACAGACATA TCAGGTAGAT TTGAAGCCAA GCGGATCAGA CATGGTTGTA 
ACCAACGACA ACAAGAAGGA ATACATCGAT CTGGTGATTC AGTGGAGGTT TGTGAACAGG 
GTCCAGAAAC AGATGAACGC CTTTTTGGAA GGCTTCACTG AACTCATTCC CATCGACTTA 
ATAAAGATTT TTGATGAAAA CGAACTGGAG CTGTTGATGT GTGGTCTGGG AGATGTGGAT 
GTCAATGACT GGAGGCAACA CACTGTCTAT AAGAACGGCT ACTGTCCAAA TCACCCCGTC 
ATCCAGTGGT TCTGGAAGGC TGTGTTGTTA ATGGATGCAG AAAAGCGCAT CAGGCTTCTA 
CAGTTTGTCA CTGGAACATC CAGAGTGCCA ATGAACGGCT TTGCTGAACT CTACGGTTCC 
AATGGGCCGC AGTTGTTTAC TATTGAGCAG TGGGGAACTC CTGACAAACT CCCTCGGGCT 
CACACCTGCT TTAATCGCTT GGACCTCCCA ATGTATGAAA CGTTTGAAGA CCTGCGAGAG 
AAGCTTCTGA TGGCGGTGGA AAACGCTCAA GGCTTTGAGG GCGTCGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nedd4l ( XM_009295229.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009293504.1 Danio rerio neural precursor cell expressed, developmentally down-regulated 4-like (nedd4l), transcript variant X4, mRNA.

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CloneID	ODa43144
Clone ID Related Accession (Same CDS sequence)	XM_009295229.2
Accession Version	XM_009295229.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3771bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	E3 ubiquitin-protein ligase NEDD4-like isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009295229.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCACC GGCTTCGGTT GTATTTTGGA TCTGGTCGCA GTAACACTGC TCCAGAGTTG 
GAAGAGCTGA ATGGGACAGT GGAGGAGGAA GGTGACATAA GCTACAACAG TACAGGAGCC 
ATGGTGGTCC ATGCACAACC ACGTAACGGA GGACATGCAG AAAGTCCAGA ACCAGGCACA 
GTTGTTGGTT CGGATGCCTC TCTAAAGCGA AGCTCTTCCA TGTTCATCCC ACAGCTCCAG 
GGTCAAGCTG AGTCACGGAC AACCAAGAGC ACCTCTGTTC AAATATCTCT TCAACGCACA 
ACTGCCCCTC ATGAAGATGC ATTACCCTTT GATCCACCTC CAGCGTACCC CCAGGATCCA 
GCTCCACCAT ACACTGAACC TGGTGAAAGT TTGCCCTTAC CTCCACCTCC AACCTACTAC 
AAATCCTGCC AAAATGTGGA ACAAACTCAA AAAGATACTG AACCTAAGAG GCAAGTCTTT 
TGTGTCCGGC CATGTGATGG TCAAAATGGT TATACCCATG GAAATCCTCA AGGGATCACT 
ATTGGAGGTG TCTGTATTAG AAGAAGATCT GCAGATGGCT CGGATGTCCA GCAGTTCAGG 
ATTATGCCAT ATAGCGAGGA TGGGCACAAT GGGCACAATT GGTCTGTCCA GCAATGCTCA 
GACAGAGGTC CTGGTGCACA AAGACTAATT TTTCAGCTCC AAAAGGACCA AGGACGGCAA 
CCAAGCTCTA CCTCTCAGCC TGACGTGAGT GATTTAGGCA TCACAGGTTC TAAAGGTTCC 
CGCTTGGTGC GGTACCCCAG GATTCGACTT GAGCGGAGCA CGTCACAGCC ATTGCAGCCT 
AGAGGAGCTC CTTCACGCCT TGGATCAATG CCAAACCTTC ATGCTCCTAT CTCAGAAATG 
GAAACGGAGA GTGATTTTAT TGAGAATGGT GCTCAAGGTT GCACTTTCAA AATTAGGAAG 
GAACAGAATT CCCAACAACC GAAGTTTAAG ATTTATTTCA GCCAGGGCGG CGAGCACAGT 
CCCATCCTCG AAAGGAATAT TAGCATTGGA AAGGCGCCTA CCTGCAGCAA TCCACAGACC 
AGAGATGACT TCCTGGGGCA GGTGGACGTG CCTCTTACAC ACTTACCGAC TGAAGACCCT 
GCCATGGAGC GTCCATACAC GTTTAAAGAC TTTCTCCTGC GTCCAAGAAG TCATAAATCT 
CGGGTGAAAG GTTACCTGAG GCTCAAGATG GCCTACCTGC CAAAGAATGG AGGACATGAG 
GAGGAAAGCG GTGAAATGAG AGAGGAGGCT GAGGGTTGGG AAGTGACAGA CTCCACAGAT 
CCAGGATCTC AGAGACCTCA GCAGCTCCTG CCTCCTCTTC CTCCGGGCTG GGAAGAGAAA 
GTGGATAACC TGGGACGGAC GTACTATGTG AACCACAACA ACCGCACCAC ACAGTGGAAG 
AGGCCCAGCA GTGTGGATGT GGTTTCAGAA ACGGAAAATG ATAATCAATT GCGGCAGATT 
AACCAGGAAG CCCACAGAGT TTTCCGCTCT CGACGGCACA TTAGCGAAGA CCTGGAAAAT 
GAGCAGCTGG ACATCAGGGA CATAGACGAC TCATGGGAGC CCATATCAGA GGAAGATCCG 
ACTTTATTAA CAGATGGTCT GAACCATTCT CTGTCGGGAC CCTCCTCTCT GGCAATGCCC 
CTCTCCAGCA CTCCAGACTT CTCAGATGAG ATCAGCCTTA GATTGTCTCT CGCTCCAGAC 
ACCAACGGAG AAATCCCGGG ACCCAGCTCT GCTGTGAGTC ATCTTTCCTC TCGGCTACGC 
TCCTCTAGTA TGACTGATGG AGTCAGTGAT CAAGCTCAAC CTCCCACACC AACGCAGAGC 
TCCCAGACCC GACGAACAAG AGCACAAACC GTCACAGGCG TTGAGGACAC CATGTCTCCT 
TCGGCCTCTT ACGCCTTGAC CACGCCAGGC CTTCCACCAG GCTGGGAGGA ACGCAAAGAC 
CCCAAGGGAC GAACGTACTA CGTTAATCAT AACAACCGTA GCACCACATG GACAAGGCCC 
ATTCTGCAGC AGCACACTGA GGATGGAGCC AGCACCTCGG TTGCAGCAGT AGGGGGCGCT 
ACAGCAGCTA CACCCCCTGC TTCAACCCCT TCCACCTCCA GCGGCCATCT AAGCGAACCA 
CAAGTGCGCA GACCCCGTAG CCTCAGTTCC CCTACCGTCA CTCTCTCTTC CCCACTAGAG 
GGAGCCAATA ACGTCCCTAT ACGGCGAGCC GTAAAGGACA CATTCTCCAA CCCTCAGTCT 
CCACAGCCTT CGCCCTACAG CTCACCTAAA ACCCAGCATA AAGCCAACCA GAGCTTCCTG 
CCACCGGGAT GGGAAATGAG GATCGCACCC AACGGACGGC CGTTCTTCAT CGACCACAAC 
AGCAGAACCA CCACATGGGA GGATCCCCGC TTGAAGTATC CCGTTCACAT GAGGACAAAA 
GCGTCTCTAG ACCCGGGCGA CCTCGGCCCA CTTCCTCCTG GGTGGGAGGA ACGAGTGCAT 
GCTGATGGAA GAACCTTTTA CATCGATCAC AACAACAAAA AAACACAGTG GGAAGACCCT 
CGTCTCCAGA GTCCTGCCAT AACTGGACCC GCGGTGCCGT ATTCAAGAGA ATTCAAGCAG 
AAATACGACT ACTTCAGGAA GAAGTTAAAG AAACCGGCAG ATATCCCCAA CCGCTTTGAG 
ATGAAGCTGC ACAGGAATAA CATCTTTGAG GAGTCCTACA GACGAATCAT GTCCCTCAAG 
AGACCTGACA GCCTGAAGGC CCGTCTGTGG ATTGAGTTTG AGTCAGAGAA GGGTCTGGAT 
TATGGCGGTG TGGCCCGAGA ATGGTTCTTC CTGCTCTCTA AGGAGATGTT CAACCCTTAT 
TATGGGCTGT TCGAGTACTC GGCCACAGAT AACTACACCC TTCAGATCAA TCCCAACTCT 
GGCCTGTGTA ATGAGGATCA CCTCTCATAC TTCAAGTTCA TTGGTCGAGT GGCCGGCATG 
GCTGTTTATC ATGGCAAACT CTTGGACGGT TTCTTCATTC GACCGTTTTA TAAGATGATG 
CTGGGCAAAC AGATCACACT GAATGACATG GAGTCAGTGG ACAGCGAATA CTACAACTCC 
CTGAAGTGGA TTCTGGAAAA CGATCCCACA GAATTAGACT TACGGTTTTG TATTGATGAG 
GACAACTTTG GACAGACATA TCAGGTAGAT TTGAAGCCAA GCGGATCAGA CATGGTTGTA 
ACCAACGACA ACAAGAAGGA ATACATCGAT CTGGTGATTC AGTGGAGGTT TGTGAACAGG 
GTCCAGAAAC AGATGAACGC CTTTTTGGAA GGCTTCACTG AACTCATTCC CATCGACTTA 
ATAAAGATTT TTGATGAAAA CGAACTGGAG CTGTTGATGT GTGGTCTGGG AGATGTGGAT 
GTCAATGACT GGAGGCAACA CACTGTCTAT AAGAACGGCT ACTGTCCAAA TCACCCCGTC 
ATCCAGTGGT TCTGGAAGGC TGTGTTGTTA ATGGATGCAG AAAAGCGCAT CAGGCTTCTA 
CAGTTTGTCA CTGGAACATC CAGAGTGCCA ATGAACGGCT TTGCTGAACT CTACGGTTCC 
AATGGGCCGC AGTTGTTTAC TATTGAGCAG TGGGGAACTC CTGACAAACT CCCTCGGGCT 
CACACCTGCT TTAATCGCTT GGACCTCCCA ATGTATGAAA CGTTTGAAGA CCTGCGAGAG 
AAGCTTCTGA TGGCGGTGGA AAACGCTCAA GGCTTTGAGG GCGTCGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009293504.1
CDS	361..4131
Translation	MSHRLRLYFGSGRSNTAPELEELNGTVEEEGDISYNSTGAMVVHAQPRNGGHAESPEPGTVVGSDASLKRSSSMFIPQLQGQAESRTTKSTSVQISLQRTTAPHEDALPFDPPPAYPQDPAPPYTEPGESLPLPPPPTYYKSCQNVEQTQKDTEPKRQVFCVRPCDGQNGYTHGNPQGITIGGVCIRRRSADGSDVQQFRIMPYSEDGHNGHNWSVQQCSDRGPGAQRLIFQLQKDQGRQPSSTSQPDVSDLGITGSKGSRLVRYPRIRLERSTSQPLQPRGAPSRLGSMPNLHAPISEMETESDFIENGAQGCTFKIRKEQNSQQPKFKIYFSQGGEHSPILERNISIGKAPTCSNPQTRDDFLGQVDVPLTHLPTEDPAMERPYTFKDFLLRPRSHKSRVKGYLRLKMAYLPKNGGHEEESGEMREEAEGWEVTDSTDPGSQRPQQLLPPLPPGWEEKVDNLGRTYYVNHNNRTTQWKRPSSVDVVSETENDNQLRQINQEAHRVFRSRRHISEDLENEQLDIRDIDDSWEPISEEDPTLLTDGLNHSLSGPSSLAMPLSSTPDFSDEISLRLSLAPDTNGEIPGPSSAVSHLSSRLRSSSMTDGVSDQAQPPTPTQSSQTRRTRAQTVTGVEDTMSPSASYALTTPGLPPGWEERKDPKGRTYYVNHNNRSTTWTRPILQQHTEDGASTSVAAVGGATAATPPASTPSTSSGHLSEPQVRRPRSLSSPTVTLSSPLEGANNVPIRRAVKDTFSNPQSPQPSPYSSPKTQHKANQSFLPPGWEMRIAPNGRPFFIDHNSRTTTWEDPRLKYPVHMRTKASLDPGDLGPLPPGWEERVHADGRTFYIDHNNKKTQWEDPRLQSPAITGPAVPYSREFKQKYDYFRKKLKKPADIPNRFEMKLHRNNIFEESYRRIMSLKRPDSLKARLWIEFESEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFKFIGRVAGMAVYHGKLLDGFFIRPFYKMMLGKQITLNDMESVDSEYYNSLKWILENDPTELDLRFCIDEDNFGQTYQVDLKPSGSDMVVTNDNKKEYIDLVIQWRFVNRVQKQMNAFLEGFTELIPIDLIKIFDENELELLMCGLGDVDVNDWRQHTVYKNGYCPNHPVIQWFWKAVLLMDAEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGTPDKLPRAHTCFNRLDLPMYETFEDLREKLLMAVENAQGFEGVD

Target ORF information:

RefSeq Version	XM_009295229.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio neural precursor cell expressed, developmentally down-regulated 4-like (nedd4l), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009295229.2

ORF Insert Sequence:

ATGTCTCACC GGCTTCGGTT GTATTTTGGA TCTGGTCGCA GTAACACTGC TCCAGAGTTG 
GAAGAGCTGA ATGGGACAGT GGAGGAGGAA GGTGACATAA GCTACAACAG TACAGGAGCC 
ATGGTGGTCC ATGCACAACC ACGTAACGGA GGACATGCAG AAAGTCCAGA ACCAGGCACA 
GTTGTTGGTT CGGATGCCTC TCTAAAGCGA AGCTCTTCCA TGTTCATCCC ACAGCTCCAG 
GGTCAAGCTG AGTCACGGAC AACCAAGAGC ACCTCTGTTC AAATATCTCT TCAACGCACA 
ACTGCCCCTC ATGAAGATGC ATTACCCTTT GATCCACCTC CAGCGTACCC CCAGGATCCA 
GCTCCACCAT ACACTGAACC TGGTGAAAGT TTGCCCTTAC CTCCACCTCC AACCTACTAC 
AAATCCTGCC AAAATGTGGA ACAAACTCAA AAAGATACTG AACCTAAGAG GCAAGTCTTT 
TGTGTCCGGC CATGTGATGG TCAAAATGGT TATACCCATG GAAATCCTCA AGGGATCACT 
ATTGGAGGTG TCTGTATTAG AAGAAGATCT GCAGATGGCT CGGATGTCCA GCAGTTCAGG 
ATTATGCCAT ATAGCGAGGA TGGGCACAAT GGGCACAATT GGTCTGTCCA GCAATGCTCA 
GACAGAGGTC CTGGTGCACA AAGACTAATT TTTCAGCTCC AAAAGGACCA AGGACGGCAA 
CCAAGCTCTA CCTCTCAGCC TGACGTGAGT GATTTAGGCA TCACAGGTTC TAAAGGTTCC 
CGCTTGGTGC GGTACCCCAG GATTCGACTT GAGCGGAGCA CGTCACAGCC ATTGCAGCCT 
AGAGGAGCTC CTTCACGCCT TGGATCAATG CCAAACCTTC ATGCTCCTAT CTCAGAAATG 
GAAACGGAGA GTGATTTTAT TGAGAATGGT GCTCAAGGTT GCACTTTCAA AATTAGGAAG 
GAACAGAATT CCCAACAACC GAAGTTTAAG ATTTATTTCA GCCAGGGCGG CGAGCACAGT 
CCCATCCTCG AAAGGAATAT TAGCATTGGA AAGGCGCCTA CCTGCAGCAA TCCACAGACC 
AGAGATGACT TCCTGGGGCA GGTGGACGTG CCTCTTACAC ACTTACCGAC TGAAGACCCT 
GCCATGGAGC GTCCATACAC GTTTAAAGAC TTTCTCCTGC GTCCAAGAAG TCATAAATCT 
CGGGTGAAAG GTTACCTGAG GCTCAAGATG GCCTACCTGC CAAAGAATGG AGGACATGAG 
GAGGAAAGCG GTGAAATGAG AGAGGAGGCT GAGGGTTGGG AAGTGACAGA CTCCACAGAT 
CCAGGATCTC AGAGACCTCA GCAGCTCCTG CCTCCTCTTC CTCCGGGCTG GGAAGAGAAA 
GTGGATAACC TGGGACGGAC GTACTATGTG AACCACAACA ACCGCACCAC ACAGTGGAAG 
AGGCCCAGCA GTGTGGATGT GGTTTCAGAA ACGGAAAATG ATAATCAATT GCGGCAGATT 
AACCAGGAAG CCCACAGAGT TTTCCGCTCT CGACGGCACA TTAGCGAAGA CCTGGAAAAT 
GAGCAGCTGG ACATCAGGGA CATAGACGAC TCATGGGAGC CCATATCAGA GGAAGATCCG 
ACTTTATTAA CAGATGGTCT GAACCATTCT CTGTCGGGAC CCTCCTCTCT GGCAATGCCC 
CTCTCCAGCA CTCCAGACTT CTCAGATGAG ATCAGCCTTA GATTGTCTCT CGCTCCAGAC 
ACCAACGGAG AAATCCCGGG ACCCAGCTCT GCTGTGAGTC ATCTTTCCTC TCGGCTACGC 
TCCTCTAGTA TGACTGATGG AGTCAGTGAT CAAGCTCAAC CTCCCACACC AACGCAGAGC 
TCCCAGACCC GACGAACAAG AGCACAAACC GTCACAGGCG TTGAGGACAC CATGTCTCCT 
TCGGCCTCTT ACGCCTTGAC CACGCCAGGC CTTCCACCAG GCTGGGAGGA ACGCAAAGAC 
CCCAAGGGAC GAACGTACTA CGTTAATCAT AACAACCGTA GCACCACATG GACAAGGCCC 
ATTCTGCAGC AGCACACTGA GGATGGAGCC AGCACCTCGG TTGCAGCAGT AGGGGGCGCT 
ACAGCAGCTA CACCCCCTGC TTCAACCCCT TCCACCTCCA GCGGCCATCT AAGCGAACCA 
CAAGTGCGCA GACCCCGTAG CCTCAGTTCC CCTACCGTCA CTCTCTCTTC CCCACTAGAG 
GGAGCCAATA ACGTCCCTAT ACGGCGAGCC GTAAAGGACA CATTCTCCAA CCCTCAGTCT 
CCACAGCCTT CGCCCTACAG CTCACCTAAA ACCCAGCATA AAGCCAACCA GAGCTTCCTG 
CCACCGGGAT GGGAAATGAG GATCGCACCC AACGGACGGC CGTTCTTCAT CGACCACAAC 
AGCAGAACCA CCACATGGGA GGATCCCCGC TTGAAGTATC CCGTTCACAT GAGGACAAAA 
GCGTCTCTAG ACCCGGGCGA CCTCGGCCCA CTTCCTCCTG GGTGGGAGGA ACGAGTGCAT 
GCTGATGGAA GAACCTTTTA CATCGATCAC AACAACAAAA AAACACAGTG GGAAGACCCT 
CGTCTCCAGA GTCCTGCCAT AACTGGACCC GCGGTGCCGT ATTCAAGAGA ATTCAAGCAG 
AAATACGACT ACTTCAGGAA GAAGTTAAAG AAACCGGCAG ATATCCCCAA CCGCTTTGAG 
ATGAAGCTGC ACAGGAATAA CATCTTTGAG GAGTCCTACA GACGAATCAT GTCCCTCAAG 
AGACCTGACA GCCTGAAGGC CCGTCTGTGG ATTGAGTTTG AGTCAGAGAA GGGTCTGGAT 
TATGGCGGTG TGGCCCGAGA ATGGTTCTTC CTGCTCTCTA AGGAGATGTT CAACCCTTAT 
TATGGGCTGT TCGAGTACTC GGCCACAGAT AACTACACCC TTCAGATCAA TCCCAACTCT 
GGCCTGTGTA ATGAGGATCA CCTCTCATAC TTCAAGTTCA TTGGTCGAGT GGCCGGCATG 
GCTGTTTATC ATGGCAAACT CTTGGACGGT TTCTTCATTC GACCGTTTTA TAAGATGATG 
CTGGGCAAAC AGATCACACT GAATGACATG GAGTCAGTGG ACAGCGAATA CTACAACTCC 
CTGAAGTGGA TTCTGGAAAA CGATCCCACA GAATTAGACT TACGGTTTTG TATTGATGAG 
GACAACTTTG GACAGACATA TCAGGTAGAT TTGAAGCCAA GCGGATCAGA CATGGTTGTA 
ACCAACGACA ACAAGAAGGA ATACATCGAT CTGGTGATTC AGTGGAGGTT TGTGAACAGG 
GTCCAGAAAC AGATGAACGC CTTTTTGGAA GGCTTCACTG AACTCATTCC CATCGACTTA 
ATAAAGATTT TTGATGAAAA CGAACTGGAG CTGTTGATGT GTGGTCTGGG AGATGTGGAT 
GTCAATGACT GGAGGCAACA CACTGTCTAT AAGAACGGCT ACTGTCCAAA TCACCCCGTC 
ATCCAGTGGT TCTGGAAGGC TGTGTTGTTA ATGGATGCAG AAAAGCGCAT CAGGCTTCTA 
CAGTTTGTCA CTGGAACATC CAGAGTGCCA ATGAACGGCT TTGCTGAACT CTACGGTTCC 
AATGGGCCGC AGTTGTTTAC TATTGAGCAG TGGGGAACTC CTGACAAACT CCCTCGGGCT 
CACACCTGCT TTAATCGCTT GGACCTCCCA ATGTATGAAA CGTTTGAAGA CCTGCGAGAG 
AAGCTTCTGA TGGCGGTGGA AAACGCTCAA GGCTTTGAGG GCGTCGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nedd4l ( XM_009295230.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009293505.1 Danio rerio neural precursor cell expressed, developmentally down-regulated 4-like (nedd4l), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ODa43145
Clone ID Related Accession (Same CDS sequence)	XM_009295230.2
Accession Version	XM_009295230.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3768bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	E3 ubiquitin-protein ligase NEDD4-like isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009295230.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCACC GGCTTCGGTT GTATTTTGGA TCTGGTCGCA GTAACACTGC TCCAGAGTTG 
GAAGAGCTGA ATGGGACAGT GGAGGAGGAA GGTGACATAA GCTACAACAG TACAGGAGCC 
ATGGTGGTCC ATGCACAACC ACGTAACGGA GGACATGCAG AAAGTCCAGA ACCAGGCACA 
GTTGTTGGTT CGGATGCCTC TCTAAAGCGA AGCTCTTCCA TGTTCATCCC ACAGCTCCAG 
GGTCAAGCTG AGTCACGGAC AACCAAGAGC ACCTCTGTTC AAATATCTCT TCAACGCACA 
ACTGCCCCTC ATGAAGATGC ATTACCCTTT GATCCACCTC CAGCGTACCC CCAGGATCCA 
GCTCCACCAT ACACTGAACC TGGTGAAAGT TTGCCCTTAC CTCCACCTCC AACCTACTAC 
AAATCCTGCC AAAATGTGGA ACAAACTCAA AAAGATACTG AACCTAAGAG GCAAGTCTTT 
TGTGTCCGGC CATGTGATGG TCAAAATGGT TATACCCATG GAAATCCTCA AGGGATCACT 
ATTGGAGGTG TCTGTATTAG AAGAAGATCT GCAGATGGCT CGGATGTCCA GCAGTTCAGG 
ATTATGCCAT ATAGCGAGGA TGGGCACAAT GGGCACAATT GGTCTGTCCA GCAATGCTCA 
GACAGAGGTC CTGGTGCACA AAGACTAATT TTTCAGCTCC AAAAGGACCA AGGACGGCAA 
CCAAGCTCTA CCTCTCAGCC TGACGTGAGT GATTTAGGCA TCACAGGTTC TAAAGGTTCC 
CGCTTGGTGC GGTACCCCAG GATTCGACTT GAGCGGAGCA CGTCACAGCC ATTGCAGCCT 
AGAGGAGCTC CTTCACGCCT TGGATCAATG CCAAACCTTC ATGCTCCTAT CTCAGAAATG 
GAAACGGAGA GTGATTTTAT TGAGAATGGT GCTCAAGGTT GCACTTTCAA AATTAGGAAG 
GAACAGAATT CCCAACAACC GAAGTTTAAG ATTTATTTCA GCCAGGGCGG CGAGCACAGT 
CCCATCCTCG AAAGGAATAT TAGCATTGGA AAGGCGCCTA CCTGCAGCAA TCCACAGACC 
AGAGATGACT TCCTGGGGCA GGTGGACGTG CCTCTTACAC ACTTACCGAC TGAAGACCCT 
GCCATGGAGC GTCCATACAC GTTTAAAGAC TTTCTCCTGC GTCCAAGAAG TCATAAATCT 
CGGGTGAAAG GTTACCTGAG GCTCAAGATG GCCTACCTGC CAAAGAATGG AGGACATGAG 
GAGGAAAGCG GTGAAATGAG AGAGGAGGCT GAGGGTTGGG AAGTGACAGA CTCCACAGAT 
CCAGGATCTC AGAGACCTCA GCAGCTCCTG CCTCCTCTTC CTCCGGGCTG GGAAGAGAAA 
GTGGATAACC TGGGACGGAC GTACTATGTG AACCACAACA ACCGCACCAC ACAGTGGAAG 
AGGCCCAGCA GTGTGGATGT GGTTTCAGAA ACGGAAAATG ATAATCAATT GCGGCAGATT 
AACCAGGAAG CCCACAGAGT TTTCCGCTCT CGACGGCACA TTAGCGAAGA CCTGGAAAAT 
GAGCAGCTGG ACATCAGGGA CATAGACGAC TCATGGGAGC CCATATCAGA GGAAGATCCG 
ACTTTATTAA CAGATGGTCT GAACCATTCT CTGTCGGGAC CCTCCTCTCT GGCAATGCCC 
CTCTCCAGCA CTCCAGACTT CTCAGATGAG ATCAGCCTTA GATTGTCTCT CGCTCCAGAC 
ACCAACGGAG AAATCCCGGG ACCCAGCTCT GCTGTGCAGA GTCATCTTTC CTCTCGGCTA 
CGCTCCTCTA GTATGACTGA TGGAGTCAGT GATCAAGCTC AACCTCCCAC ACCAACGAGC 
TCCCAGACCC GACGAACAAG AGCACAAACC GTCACAGGCG TTGAGGACAC CATGTCTCCT 
TCGGCCTCTT ACGCCTTGAC CACGCCAGGC CTTCCACCAG GCTGGGAGGA ACGCAAAGAC 
CCCAAGGGAC GAACGTACTA CGTTAATCAT AACAACCGTA GCACCACATG GACAAGGCCC 
ATTCTGCAGC ACACTGAGGA TGGAGCCAGC ACCTCGGTTG CAGCAGTAGG GGGCGCTACA 
GCAGCTACAC CCCCTGCTTC AACCCCTTCC ACCTCCAGCG GCCATCTAAG CGAACCACAA 
GTGCGCAGAC CCCGTAGCCT CAGTTCCCCT ACCGTCACTC TCTCTTCCCC ACTAGAGGGA 
GCCAATAACG TCCCTATACG GCGAGCCGTA AAGGACACAT TCTCCAACCC TCAGTCTCCA 
CAGCCTTCGC CCTACAGCTC ACCTAAAACC CAGCATAAAG CCAACCAGAG CTTCCTGCCA 
CCGGGATGGG AAATGAGGAT CGCACCCAAC GGACGGCCGT TCTTCATCGA CCACAACAGC 
AGAACCACCA CATGGGAGGA TCCCCGCTTG AAGTATCCCG TTCACATGAG GACAAAAGCG 
TCTCTAGACC CGGGCGACCT CGGCCCACTT CCTCCTGGGT GGGAGGAACG AGTGCATGCT 
GATGGAAGAA CCTTTTACAT CGATCACAAC AACAAAAAAA CACAGTGGGA AGACCCTCGT 
CTCCAGAGTC CTGCCATAAC TGGACCCGCG GTGCCGTATT CAAGAGAATT CAAGCAGAAA 
TACGACTACT TCAGGAAGAA GTTAAAGAAA CCGGCAGATA TCCCCAACCG CTTTGAGATG 
AAGCTGCACA GGAATAACAT CTTTGAGGAG TCCTACAGAC GAATCATGTC CCTCAAGAGA 
CCTGACAGCC TGAAGGCCCG TCTGTGGATT GAGTTTGAGT CAGAGAAGGG TCTGGATTAT 
GGCGGTGTGG CCCGAGAATG GTTCTTCCTG CTCTCTAAGG AGATGTTCAA CCCTTATTAT 
GGGCTGTTCG AGTACTCGGC CACAGATAAC TACACCCTTC AGATCAATCC CAACTCTGGC 
CTGTGTAATG AGGATCACCT CTCATACTTC AAGTTCATTG GTCGAGTGGC CGGCATGGCT 
GTTTATCATG GCAAACTCTT GGACGGTTTC TTCATTCGAC CGTTTTATAA GATGATGCTG 
GGCAAACAGA TCACACTGAA TGACATGGAG TCAGTGGACA GCGAATACTA CAACTCCCTG 
AAGTGGATTC TGGAAAACGA TCCCACAGAA TTAGACTTAC GGTTTTGTAT TGATGAGGAC 
AACTTTGGAC AGACATATCA GGTAGATTTG AAGCCAAGCG GATCAGACAT GGTTGTAACC 
AACGACAACA AGAAGGAATA CATCGATCTG GTGATTCAGT GGAGGTTTGT GAACAGGGTC 
CAGAAACAGA TGAACGCCTT TTTGGAAGGC TTCACTGAAC TCATTCCCAT CGACTTAATA 
AAGATTTTTG ATGAAAACGA ACTGGAGCTG TTGATGTGTG GTCTGGGAGA TGTGGATGTC 
AATGACTGGA GGCAACACAC TGTCTATAAG AACGGCTACT GTCCAAATCA CCCCGTCATC 
CAGTGGTTCT GGAAGGCTGT GTTGTTAATG GATGCAGAAA AGCGCATCAG GCTTCTACAG 
TTTGTCACTG GAACATCCAG AGTGCCAATG AACGGCTTTG CTGAACTCTA CGGTTCCAAT 
GGGCCGCAGT TGTTTACTAT TGAGCAGTGG GGAACTCCTG ACAAACTCCC TCGGGCTCAC 
ACCTGCTTTA ATCGCTTGGA CCTCCCAATG TATGAAACGT TTGAAGACCT GCGAGAGAAG 
CTTCTGATGG CGGTGGAAAA CGCTCAAGGC TTTGAGGGCG TCGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009293505.1
CDS	358..4125
Translation	MSHRLRLYFGSGRSNTAPELEELNGTVEEEGDISYNSTGAMVVHAQPRNGGHAESPEPGTVVGSDASLKRSSSMFIPQLQGQAESRTTKSTSVQISLQRTTAPHEDALPFDPPPAYPQDPAPPYTEPGESLPLPPPPTYYKSCQNVEQTQKDTEPKRQVFCVRPCDGQNGYTHGNPQGITIGGVCIRRRSADGSDVQQFRIMPYSEDGHNGHNWSVQQCSDRGPGAQRLIFQLQKDQGRQPSSTSQPDVSDLGITGSKGSRLVRYPRIRLERSTSQPLQPRGAPSRLGSMPNLHAPISEMETESDFIENGAQGCTFKIRKEQNSQQPKFKIYFSQGGEHSPILERNISIGKAPTCSNPQTRDDFLGQVDVPLTHLPTEDPAMERPYTFKDFLLRPRSHKSRVKGYLRLKMAYLPKNGGHEEESGEMREEAEGWEVTDSTDPGSQRPQQLLPPLPPGWEEKVDNLGRTYYVNHNNRTTQWKRPSSVDVVSETENDNQLRQINQEAHRVFRSRRHISEDLENEQLDIRDIDDSWEPISEEDPTLLTDGLNHSLSGPSSLAMPLSSTPDFSDEISLRLSLAPDTNGEIPGPSSAVQSHLSSRLRSSSMTDGVSDQAQPPTPTSSQTRRTRAQTVTGVEDTMSPSASYALTTPGLPPGWEERKDPKGRTYYVNHNNRSTTWTRPILQHTEDGASTSVAAVGGATAATPPASTPSTSSGHLSEPQVRRPRSLSSPTVTLSSPLEGANNVPIRRAVKDTFSNPQSPQPSPYSSPKTQHKANQSFLPPGWEMRIAPNGRPFFIDHNSRTTTWEDPRLKYPVHMRTKASLDPGDLGPLPPGWEERVHADGRTFYIDHNNKKTQWEDPRLQSPAITGPAVPYSREFKQKYDYFRKKLKKPADIPNRFEMKLHRNNIFEESYRRIMSLKRPDSLKARLWIEFESEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFKFIGRVAGMAVYHGKLLDGFFIRPFYKMMLGKQITLNDMESVDSEYYNSLKWILENDPTELDLRFCIDEDNFGQTYQVDLKPSGSDMVVTNDNKKEYIDLVIQWRFVNRVQKQMNAFLEGFTELIPIDLIKIFDENELELLMCGLGDVDVNDWRQHTVYKNGYCPNHPVIQWFWKAVLLMDAEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGTPDKLPRAHTCFNRLDLPMYETFEDLREKLLMAVENAQGFEGVD

Target ORF information:

RefSeq Version	XM_009295230.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio neural precursor cell expressed, developmentally down-regulated 4-like (nedd4l), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009295230.2

ORF Insert Sequence:

ATGTCTCACC GGCTTCGGTT GTATTTTGGA TCTGGTCGCA GTAACACTGC TCCAGAGTTG 
GAAGAGCTGA ATGGGACAGT GGAGGAGGAA GGTGACATAA GCTACAACAG TACAGGAGCC 
ATGGTGGTCC ATGCACAACC ACGTAACGGA GGACATGCAG AAAGTCCAGA ACCAGGCACA 
GTTGTTGGTT CGGATGCCTC TCTAAAGCGA AGCTCTTCCA TGTTCATCCC ACAGCTCCAG 
GGTCAAGCTG AGTCACGGAC AACCAAGAGC ACCTCTGTTC AAATATCTCT TCAACGCACA 
ACTGCCCCTC ATGAAGATGC ATTACCCTTT GATCCACCTC CAGCGTACCC CCAGGATCCA 
GCTCCACCAT ACACTGAACC TGGTGAAAGT TTGCCCTTAC CTCCACCTCC AACCTACTAC 
AAATCCTGCC AAAATGTGGA ACAAACTCAA AAAGATACTG AACCTAAGAG GCAAGTCTTT 
TGTGTCCGGC CATGTGATGG TCAAAATGGT TATACCCATG GAAATCCTCA AGGGATCACT 
ATTGGAGGTG TCTGTATTAG AAGAAGATCT GCAGATGGCT CGGATGTCCA GCAGTTCAGG 
ATTATGCCAT ATAGCGAGGA TGGGCACAAT GGGCACAATT GGTCTGTCCA GCAATGCTCA 
GACAGAGGTC CTGGTGCACA AAGACTAATT TTTCAGCTCC AAAAGGACCA AGGACGGCAA 
CCAAGCTCTA CCTCTCAGCC TGACGTGAGT GATTTAGGCA TCACAGGTTC TAAAGGTTCC 
CGCTTGGTGC GGTACCCCAG GATTCGACTT GAGCGGAGCA CGTCACAGCC ATTGCAGCCT 
AGAGGAGCTC CTTCACGCCT TGGATCAATG CCAAACCTTC ATGCTCCTAT CTCAGAAATG 
GAAACGGAGA GTGATTTTAT TGAGAATGGT GCTCAAGGTT GCACTTTCAA AATTAGGAAG 
GAACAGAATT CCCAACAACC GAAGTTTAAG ATTTATTTCA GCCAGGGCGG CGAGCACAGT 
CCCATCCTCG AAAGGAATAT TAGCATTGGA AAGGCGCCTA CCTGCAGCAA TCCACAGACC 
AGAGATGACT TCCTGGGGCA GGTGGACGTG CCTCTTACAC ACTTACCGAC TGAAGACCCT 
GCCATGGAGC GTCCATACAC GTTTAAAGAC TTTCTCCTGC GTCCAAGAAG TCATAAATCT 
CGGGTGAAAG GTTACCTGAG GCTCAAGATG GCCTACCTGC CAAAGAATGG AGGACATGAG 
GAGGAAAGCG GTGAAATGAG AGAGGAGGCT GAGGGTTGGG AAGTGACAGA CTCCACAGAT 
CCAGGATCTC AGAGACCTCA GCAGCTCCTG CCTCCTCTTC CTCCGGGCTG GGAAGAGAAA 
GTGGATAACC TGGGACGGAC GTACTATGTG AACCACAACA ACCGCACCAC ACAGTGGAAG 
AGGCCCAGCA GTGTGGATGT GGTTTCAGAA ACGGAAAATG ATAATCAATT GCGGCAGATT 
AACCAGGAAG CCCACAGAGT TTTCCGCTCT CGACGGCACA TTAGCGAAGA CCTGGAAAAT 
GAGCAGCTGG ACATCAGGGA CATAGACGAC TCATGGGAGC CCATATCAGA GGAAGATCCG 
ACTTTATTAA CAGATGGTCT GAACCATTCT CTGTCGGGAC CCTCCTCTCT GGCAATGCCC 
CTCTCCAGCA CTCCAGACTT CTCAGATGAG ATCAGCCTTA GATTGTCTCT CGCTCCAGAC 
ACCAACGGAG AAATCCCGGG ACCCAGCTCT GCTGTGCAGA GTCATCTTTC CTCTCGGCTA 
CGCTCCTCTA GTATGACTGA TGGAGTCAGT GATCAAGCTC AACCTCCCAC ACCAACGAGC 
TCCCAGACCC GACGAACAAG AGCACAAACC GTCACAGGCG TTGAGGACAC CATGTCTCCT 
TCGGCCTCTT ACGCCTTGAC CACGCCAGGC CTTCCACCAG GCTGGGAGGA ACGCAAAGAC 
CCCAAGGGAC GAACGTACTA CGTTAATCAT AACAACCGTA GCACCACATG GACAAGGCCC 
ATTCTGCAGC ACACTGAGGA TGGAGCCAGC ACCTCGGTTG CAGCAGTAGG GGGCGCTACA 
GCAGCTACAC CCCCTGCTTC AACCCCTTCC ACCTCCAGCG GCCATCTAAG CGAACCACAA 
GTGCGCAGAC CCCGTAGCCT CAGTTCCCCT ACCGTCACTC TCTCTTCCCC ACTAGAGGGA 
GCCAATAACG TCCCTATACG GCGAGCCGTA AAGGACACAT TCTCCAACCC TCAGTCTCCA 
CAGCCTTCGC CCTACAGCTC ACCTAAAACC CAGCATAAAG CCAACCAGAG CTTCCTGCCA 
CCGGGATGGG AAATGAGGAT CGCACCCAAC GGACGGCCGT TCTTCATCGA CCACAACAGC 
AGAACCACCA CATGGGAGGA TCCCCGCTTG AAGTATCCCG TTCACATGAG GACAAAAGCG 
TCTCTAGACC CGGGCGACCT CGGCCCACTT CCTCCTGGGT GGGAGGAACG AGTGCATGCT 
GATGGAAGAA CCTTTTACAT CGATCACAAC AACAAAAAAA CACAGTGGGA AGACCCTCGT 
CTCCAGAGTC CTGCCATAAC TGGACCCGCG GTGCCGTATT CAAGAGAATT CAAGCAGAAA 
TACGACTACT TCAGGAAGAA GTTAAAGAAA CCGGCAGATA TCCCCAACCG CTTTGAGATG 
AAGCTGCACA GGAATAACAT CTTTGAGGAG TCCTACAGAC GAATCATGTC CCTCAAGAGA 
CCTGACAGCC TGAAGGCCCG TCTGTGGATT GAGTTTGAGT CAGAGAAGGG TCTGGATTAT 
GGCGGTGTGG CCCGAGAATG GTTCTTCCTG CTCTCTAAGG AGATGTTCAA CCCTTATTAT 
GGGCTGTTCG AGTACTCGGC CACAGATAAC TACACCCTTC AGATCAATCC CAACTCTGGC 
CTGTGTAATG AGGATCACCT CTCATACTTC AAGTTCATTG GTCGAGTGGC CGGCATGGCT 
GTTTATCATG GCAAACTCTT GGACGGTTTC TTCATTCGAC CGTTTTATAA GATGATGCTG 
GGCAAACAGA TCACACTGAA TGACATGGAG TCAGTGGACA GCGAATACTA CAACTCCCTG 
AAGTGGATTC TGGAAAACGA TCCCACAGAA TTAGACTTAC GGTTTTGTAT TGATGAGGAC 
AACTTTGGAC AGACATATCA GGTAGATTTG AAGCCAAGCG GATCAGACAT GGTTGTAACC 
AACGACAACA AGAAGGAATA CATCGATCTG GTGATTCAGT GGAGGTTTGT GAACAGGGTC 
CAGAAACAGA TGAACGCCTT TTTGGAAGGC TTCACTGAAC TCATTCCCAT CGACTTAATA 
AAGATTTTTG ATGAAAACGA ACTGGAGCTG TTGATGTGTG GTCTGGGAGA TGTGGATGTC 
AATGACTGGA GGCAACACAC TGTCTATAAG AACGGCTACT GTCCAAATCA CCCCGTCATC 
CAGTGGTTCT GGAAGGCTGT GTTGTTAATG GATGCAGAAA AGCGCATCAG GCTTCTACAG 
TTTGTCACTG GAACATCCAG AGTGCCAATG AACGGCTTTG CTGAACTCTA CGGTTCCAAT 
GGGCCGCAGT TGTTTACTAT TGAGCAGTGG GGAACTCCTG ACAAACTCCC TCGGGCTCAC 
ACCTGCTTTA ATCGCTTGGA CCTCCCAATG TATGAAACGT TTGAAGACCT GCGAGAGAAG 
CTTCTGATGG CGGTGGAAAA CGCTCAAGGC TTTGAGGGCG TCGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nedd4l ( XM_009295231.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009293506.1 Danio rerio neural precursor cell expressed, developmentally down-regulated 4-like (nedd4l), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ODa43146
Clone ID Related Accession (Same CDS sequence)	XM_009295231.2
Accession Version	XM_009295231.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3714bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	E3 ubiquitin-protein ligase NEDD4-like isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009295231.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCACC GGCTTCGGTT GTATTTTGGA TCTGGTCGCA GTAACACTGC TCCAGAGTTG 
GAAGAGCTGA ATGGGACAGT GGAGGAGGAA GGTGACATAA GCTACAACAG TACAGGAGCC 
ATGGTGGTCC ATGCACAACC ACGTAACGGA GGACATGCAG AAAGTCCAGA ACCAGGCACA 
GTTGTTGGTT CGGATGCCTC TCTAAAGCGA AGCTCTTCCA TGTTCATCCC ACAGCTCCAG 
GGTCAAGCTG AGTCACGGAC AACCAAGAGC ACCTCTGTTC AAATATCTCT TCAACGCACA 
ACTGCCCCTC ATGAAGATGC ATTACCCTTT GATCCACCTC CAGCGTACCC CCAGGATCCA 
GCTCCACCAT ACACTGAACC TGGTGAAAGT TTGCCCTTAC CTCCACCTCC AACCTACTAC 
AAATCCTGCC AAAATGTGGA ACAAACTCAA AAAGATACTG AACCTAAGAG GCAAGTCTTT 
TGTGTCCGGC CATGTGATGG TCAAAATGGT TATACCCATG GAAATCCTCA AGGGATCACT 
ATTGGAGGTG TCTGTATTAG AAGAAGATCT GCAGATGGCT CGGATGTCCA GCAGTTCAGG 
ATTATGCCAT ATAGCGAGGA TGGGCACAAT GGGCACAATT GGTCTGTCCA GCAATGCTCA 
GACAGAGGTC CTGGTGCACA AAGACTAATT TTTCAGCTCC AAAAGGACCA AGGACGGCAA 
CCAAGCTCTA CCTCTCAGCC TGACGTGAGT GATTTAGGCA TCACAGGTTC TAAAGGTTCC 
CGCTTGGTGC GGTACCCCAG GATTCGACTT GAGCGGAGCA CGTCACAGCC ATTGCAGCCT 
AGAGGAGCTC CTTCACGCCT TGGATCAATG CCAAACCTTC ATGCTCCTAT CTCAGAAATG 
GAAACGGAGA GTGATTTTAT TGAGAATGGT GCTCAAGGTT GCACTTTCAA AATTAGGAAG 
GAACAGAATT CCCAACAACC GAAGTTTAAG ATTTATTTCA GCCAGGGCGG CGAGCACAGT 
CCCATCCTCG AAAGGAATAT TAGCATTGGA AAGGCGCCTA CCTGCAGCAA TCCACAGACC 
AGAGATGACT TCCTGGGGCA GGTGGACGTG CCTCTTACAC ACTTACCGAC TGAAGACCCT 
GCCATGGAGC GTCCATACAC GTTTAAAGAC TTTCTCCTGC GTCCAAGAAG TCATAAATCT 
CGGGTGAAAG GTTACCTGAG GCTCAAGATG GCCTACCTGC CAAAGAATGG AGGACATGAG 
GAGGAAAGCG GTGAAATGAG AGAGGAGGCT GAGGGTTGGG AAGTGACAGA CTCCACAGAT 
CCAGGATCTC AGAGACCTCA GCAGCTCCTG CCTCCTCTTC CTCCGGGCTG GGAAGAGAAA 
GTGGATAACC TGGGACGGAC GTACTATGTG AACCACAACA ACCGCACCAC ACAGTGGAAG 
AGGCCCAGCA GTGTGGATGT GGTTTCAGAA ACGGAAAATG ATAATCAATT GCGGCAGATT 
AACCAGGAAG CCCACAGAGT TTTCCGCTCT CGACGGCACA TTAGCGAAGA CCTGGAAAAT 
GAGCAGCTGG ACATCAGGGA CATAGACGAC TCATGGGAGC CCATATCAGA GGAAGATCCG 
ACTTTATTAA CAGATGGTCT GAACCATTCT CTGTCGGGAC CCTCCTCTCT GGCAATGCCC 
CTCTCCAGCA CTCCAGACTT CTCAGATGAG ATCAGCCTTA GATTGTCTCT CGCTCCAGAC 
ACCAACGGAG AAATCCCGGG ACCCAGCTCT GCTGTGCAGA GTCATCTTTC CTCTCGGCTA 
CGCTCCTCTA GTATGACTGA TGGAGTCAGT GATCAAGCTC AACCTCCCAC ACCAACGTCT 
CCTTCGGCCT CTTACGCCTT GACCACGCCA GGCCTTCCAC CAGGCTGGGA GGAACGCAAA 
GACCCCAAGG GACGAACGTA CTACGTTAAT CATAACAACC GTAGCACCAC ATGGACAAGG 
CCCATTCTGC AGCAGCACAC TGAGGATGGA GCCAGCACCT CGGTTGCAGC AGTAGGGGGC 
GCTACAGCAG CTACACCCCC TGCTTCAACC CCTTCCACCT CCAGCGGCCA TCTAAGCGAA 
CCACAAGTGC GCAGACCCCG TAGCCTCAGT TCCCCTACCG TCACTCTCTC TTCCCCACTA 
GAGGGAGCCA ATAACGTCCC TATACGGCGA GCCGTAAAGG ACACATTCTC CAACCCTCAG 
TCTCCACAGC CTTCGCCCTA CAGCTCACCT AAAACCCAGC ATAAAGCCAA CCAGAGCTTC 
CTGCCACCGG GATGGGAAAT GAGGATCGCA CCCAACGGAC GGCCGTTCTT CATCGACCAC 
AACAGCAGAA CCACCACATG GGAGGATCCC CGCTTGAAGT ATCCCGTTCA CATGAGGACA 
AAAGCGTCTC TAGACCCGGG CGACCTCGGC CCACTTCCTC CTGGGTGGGA GGAACGAGTG 
CATGCTGATG GAAGAACCTT TTACATCGAT CACAACAACA AAAAAACACA GTGGGAAGAC 
CCTCGTCTCC AGAGTCCTGC CATAACTGGA CCCGCGGTGC CGTATTCAAG AGAATTCAAG 
CAGAAATACG ACTACTTCAG GAAGAAGTTA AAGAAACCGG CAGATATCCC CAACCGCTTT 
GAGATGAAGC TGCACAGGAA TAACATCTTT GAGGAGTCCT ACAGACGAAT CATGTCCCTC 
AAGAGACCTG ACAGCCTGAA GGCCCGTCTG TGGATTGAGT TTGAGTCAGA GAAGGGTCTG 
GATTATGGCG GTGTGGCCCG AGAATGGTTC TTCCTGCTCT CTAAGGAGAT GTTCAACCCT 
TATTATGGGC TGTTCGAGTA CTCGGCCACA GATAACTACA CCCTTCAGAT CAATCCCAAC 
TCTGGCCTGT GTAATGAGGA TCACCTCTCA TACTTCAAGT TCATTGGTCG AGTGGCCGGC 
ATGGCTGTTT ATCATGGCAA ACTCTTGGAC GGTTTCTTCA TTCGACCGTT TTATAAGATG 
ATGCTGGGCA AACAGATCAC ACTGAATGAC ATGGAGTCAG TGGACAGCGA ATACTACAAC 
TCCCTGAAGT GGATTCTGGA AAACGATCCC ACAGAATTAG ACTTACGGTT TTGTATTGAT 
GAGGACAACT TTGGACAGAC ATATCAGGTA GATTTGAAGC CAAGCGGATC AGACATGGTT 
GTAACCAACG ACAACAAGAA GGAATACATC GATCTGGTGA TTCAGTGGAG GTTTGTGAAC 
AGGGTCCAGA AACAGATGAA CGCCTTTTTG GAAGGCTTCA CTGAACTCAT TCCCATCGAC 
TTAATAAAGA TTTTTGATGA AAACGAACTG GAGCTGTTGA TGTGTGGTCT GGGAGATGTG 
GATGTCAATG ACTGGAGGCA ACACACTGTC TATAAGAACG GCTACTGTCC AAATCACCCC 
GTCATCCAGT GGTTCTGGAA GGCTGTGTTG TTAATGGATG CAGAAAAGCG CATCAGGCTT 
CTACAGTTTG TCACTGGAAC ATCCAGAGTG CCAATGAACG GCTTTGCTGA ACTCTACGGT 
TCCAATGGGC CGCAGTTGTT TACTATTGAG CAGTGGGGAA CTCCTGACAA ACTCCCTCGG 
GCTCACACCT GCTTTAATCG CTTGGACCTC CCAATGTATG AAACGTTTGA AGACCTGCGA 
GAGAAGCTTC TGATGGCGGT GGAAAACGCT CAAGGCTTTG AGGGCGTCGA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009293506.1
CDS	359..4072
Translation	MSHRLRLYFGSGRSNTAPELEELNGTVEEEGDISYNSTGAMVVHAQPRNGGHAESPEPGTVVGSDASLKRSSSMFIPQLQGQAESRTTKSTSVQISLQRTTAPHEDALPFDPPPAYPQDPAPPYTEPGESLPLPPPPTYYKSCQNVEQTQKDTEPKRQVFCVRPCDGQNGYTHGNPQGITIGGVCIRRRSADGSDVQQFRIMPYSEDGHNGHNWSVQQCSDRGPGAQRLIFQLQKDQGRQPSSTSQPDVSDLGITGSKGSRLVRYPRIRLERSTSQPLQPRGAPSRLGSMPNLHAPISEMETESDFIENGAQGCTFKIRKEQNSQQPKFKIYFSQGGEHSPILERNISIGKAPTCSNPQTRDDFLGQVDVPLTHLPTEDPAMERPYTFKDFLLRPRSHKSRVKGYLRLKMAYLPKNGGHEEESGEMREEAEGWEVTDSTDPGSQRPQQLLPPLPPGWEEKVDNLGRTYYVNHNNRTTQWKRPSSVDVVSETENDNQLRQINQEAHRVFRSRRHISEDLENEQLDIRDIDDSWEPISEEDPTLLTDGLNHSLSGPSSLAMPLSSTPDFSDEISLRLSLAPDTNGEIPGPSSAVQSHLSSRLRSSSMTDGVSDQAQPPTPTSPSASYALTTPGLPPGWEERKDPKGRTYYVNHNNRSTTWTRPILQQHTEDGASTSVAAVGGATAATPPASTPSTSSGHLSEPQVRRPRSLSSPTVTLSSPLEGANNVPIRRAVKDTFSNPQSPQPSPYSSPKTQHKANQSFLPPGWEMRIAPNGRPFFIDHNSRTTTWEDPRLKYPVHMRTKASLDPGDLGPLPPGWEERVHADGRTFYIDHNNKKTQWEDPRLQSPAITGPAVPYSREFKQKYDYFRKKLKKPADIPNRFEMKLHRNNIFEESYRRIMSLKRPDSLKARLWIEFESEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFKFIGRVAGMAVYHGKLLDGFFIRPFYKMMLGKQITLNDMESVDSEYYNSLKWILENDPTELDLRFCIDEDNFGQTYQVDLKPSGSDMVVTNDNKKEYIDLVIQWRFVNRVQKQMNAFLEGFTELIPIDLIKIFDENELELLMCGLGDVDVNDWRQHTVYKNGYCPNHPVIQWFWKAVLLMDAEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGTPDKLPRAHTCFNRLDLPMYETFEDLREKLLMAVENAQGFEGVD

Target ORF information:

RefSeq Version	XM_009295231.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio neural precursor cell expressed, developmentally down-regulated 4-like (nedd4l), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009295231.2

ORF Insert Sequence:

ATGTCTCACC GGCTTCGGTT GTATTTTGGA TCTGGTCGCA GTAACACTGC TCCAGAGTTG 
GAAGAGCTGA ATGGGACAGT GGAGGAGGAA GGTGACATAA GCTACAACAG TACAGGAGCC 
ATGGTGGTCC ATGCACAACC ACGTAACGGA GGACATGCAG AAAGTCCAGA ACCAGGCACA 
GTTGTTGGTT CGGATGCCTC TCTAAAGCGA AGCTCTTCCA TGTTCATCCC ACAGCTCCAG 
GGTCAAGCTG AGTCACGGAC AACCAAGAGC ACCTCTGTTC AAATATCTCT TCAACGCACA 
ACTGCCCCTC ATGAAGATGC ATTACCCTTT GATCCACCTC CAGCGTACCC CCAGGATCCA 
GCTCCACCAT ACACTGAACC TGGTGAAAGT TTGCCCTTAC CTCCACCTCC AACCTACTAC 
AAATCCTGCC AAAATGTGGA ACAAACTCAA AAAGATACTG AACCTAAGAG GCAAGTCTTT 
TGTGTCCGGC CATGTGATGG TCAAAATGGT TATACCCATG GAAATCCTCA AGGGATCACT 
ATTGGAGGTG TCTGTATTAG AAGAAGATCT GCAGATGGCT CGGATGTCCA GCAGTTCAGG 
ATTATGCCAT ATAGCGAGGA TGGGCACAAT GGGCACAATT GGTCTGTCCA GCAATGCTCA 
GACAGAGGTC CTGGTGCACA AAGACTAATT TTTCAGCTCC AAAAGGACCA AGGACGGCAA 
CCAAGCTCTA CCTCTCAGCC TGACGTGAGT GATTTAGGCA TCACAGGTTC TAAAGGTTCC 
CGCTTGGTGC GGTACCCCAG GATTCGACTT GAGCGGAGCA CGTCACAGCC ATTGCAGCCT 
AGAGGAGCTC CTTCACGCCT TGGATCAATG CCAAACCTTC ATGCTCCTAT CTCAGAAATG 
GAAACGGAGA GTGATTTTAT TGAGAATGGT GCTCAAGGTT GCACTTTCAA AATTAGGAAG 
GAACAGAATT CCCAACAACC GAAGTTTAAG ATTTATTTCA GCCAGGGCGG CGAGCACAGT 
CCCATCCTCG AAAGGAATAT TAGCATTGGA AAGGCGCCTA CCTGCAGCAA TCCACAGACC 
AGAGATGACT TCCTGGGGCA GGTGGACGTG CCTCTTACAC ACTTACCGAC TGAAGACCCT 
GCCATGGAGC GTCCATACAC GTTTAAAGAC TTTCTCCTGC GTCCAAGAAG TCATAAATCT 
CGGGTGAAAG GTTACCTGAG GCTCAAGATG GCCTACCTGC CAAAGAATGG AGGACATGAG 
GAGGAAAGCG GTGAAATGAG AGAGGAGGCT GAGGGTTGGG AAGTGACAGA CTCCACAGAT 
CCAGGATCTC AGAGACCTCA GCAGCTCCTG CCTCCTCTTC CTCCGGGCTG GGAAGAGAAA 
GTGGATAACC TGGGACGGAC GTACTATGTG AACCACAACA ACCGCACCAC ACAGTGGAAG 
AGGCCCAGCA GTGTGGATGT GGTTTCAGAA ACGGAAAATG ATAATCAATT GCGGCAGATT 
AACCAGGAAG CCCACAGAGT TTTCCGCTCT CGACGGCACA TTAGCGAAGA CCTGGAAAAT 
GAGCAGCTGG ACATCAGGGA CATAGACGAC TCATGGGAGC CCATATCAGA GGAAGATCCG 
ACTTTATTAA CAGATGGTCT GAACCATTCT CTGTCGGGAC CCTCCTCTCT GGCAATGCCC 
CTCTCCAGCA CTCCAGACTT CTCAGATGAG ATCAGCCTTA GATTGTCTCT CGCTCCAGAC 
ACCAACGGAG AAATCCCGGG ACCCAGCTCT GCTGTGCAGA GTCATCTTTC CTCTCGGCTA 
CGCTCCTCTA GTATGACTGA TGGAGTCAGT GATCAAGCTC AACCTCCCAC ACCAACGTCT 
CCTTCGGCCT CTTACGCCTT GACCACGCCA GGCCTTCCAC CAGGCTGGGA GGAACGCAAA 
GACCCCAAGG GACGAACGTA CTACGTTAAT CATAACAACC GTAGCACCAC ATGGACAAGG 
CCCATTCTGC AGCAGCACAC TGAGGATGGA GCCAGCACCT CGGTTGCAGC AGTAGGGGGC 
GCTACAGCAG CTACACCCCC TGCTTCAACC CCTTCCACCT CCAGCGGCCA TCTAAGCGAA 
CCACAAGTGC GCAGACCCCG TAGCCTCAGT TCCCCTACCG TCACTCTCTC TTCCCCACTA 
GAGGGAGCCA ATAACGTCCC TATACGGCGA GCCGTAAAGG ACACATTCTC CAACCCTCAG 
TCTCCACAGC CTTCGCCCTA CAGCTCACCT AAAACCCAGC ATAAAGCCAA CCAGAGCTTC 
CTGCCACCGG GATGGGAAAT GAGGATCGCA CCCAACGGAC GGCCGTTCTT CATCGACCAC 
AACAGCAGAA CCACCACATG GGAGGATCCC CGCTTGAAGT ATCCCGTTCA CATGAGGACA 
AAAGCGTCTC TAGACCCGGG CGACCTCGGC CCACTTCCTC CTGGGTGGGA GGAACGAGTG 
CATGCTGATG GAAGAACCTT TTACATCGAT CACAACAACA AAAAAACACA GTGGGAAGAC 
CCTCGTCTCC AGAGTCCTGC CATAACTGGA CCCGCGGTGC CGTATTCAAG AGAATTCAAG 
CAGAAATACG ACTACTTCAG GAAGAAGTTA AAGAAACCGG CAGATATCCC CAACCGCTTT 
GAGATGAAGC TGCACAGGAA TAACATCTTT GAGGAGTCCT ACAGACGAAT CATGTCCCTC 
AAGAGACCTG ACAGCCTGAA GGCCCGTCTG TGGATTGAGT TTGAGTCAGA GAAGGGTCTG 
GATTATGGCG GTGTGGCCCG AGAATGGTTC TTCCTGCTCT CTAAGGAGAT GTTCAACCCT 
TATTATGGGC TGTTCGAGTA CTCGGCCACA GATAACTACA CCCTTCAGAT CAATCCCAAC 
TCTGGCCTGT GTAATGAGGA TCACCTCTCA TACTTCAAGT TCATTGGTCG AGTGGCCGGC 
ATGGCTGTTT ATCATGGCAA ACTCTTGGAC GGTTTCTTCA TTCGACCGTT TTATAAGATG 
ATGCTGGGCA AACAGATCAC ACTGAATGAC ATGGAGTCAG TGGACAGCGA ATACTACAAC 
TCCCTGAAGT GGATTCTGGA AAACGATCCC ACAGAATTAG ACTTACGGTT TTGTATTGAT 
GAGGACAACT TTGGACAGAC ATATCAGGTA GATTTGAAGC CAAGCGGATC AGACATGGTT 
GTAACCAACG ACAACAAGAA GGAATACATC GATCTGGTGA TTCAGTGGAG GTTTGTGAAC 
AGGGTCCAGA AACAGATGAA CGCCTTTTTG GAAGGCTTCA CTGAACTCAT TCCCATCGAC 
TTAATAAAGA TTTTTGATGA AAACGAACTG GAGCTGTTGA TGTGTGGTCT GGGAGATGTG 
GATGTCAATG ACTGGAGGCA ACACACTGTC TATAAGAACG GCTACTGTCC AAATCACCCC 
GTCATCCAGT GGTTCTGGAA GGCTGTGTTG TTAATGGATG CAGAAAAGCG CATCAGGCTT 
CTACAGTTTG TCACTGGAAC ATCCAGAGTG CCAATGAACG GCTTTGCTGA ACTCTACGGT 
TCCAATGGGC CGCAGTTGTT TACTATTGAG CAGTGGGGAA CTCCTGACAA ACTCCCTCGG 
GCTCACACCT GCTTTAATCG CTTGGACCTC CCAATGTATG AAACGTTTGA AGACCTGCGA 
GAGAAGCTTC TGATGGCGGT GGAAAACGCT CAAGGCTTTG AGGGCGTCGA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	nedd4l
Entrez Gene ID	559634
Full Name	neural precursor cell expressed, developmentally down-regulated 4-like
General protein information	Preferred Names neural precursor cell expressed, developmentally down-regulated 4-like Names E3 ubiquitin-protein ligase NEDD4-like nedd4b
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

Pan troglodytes (chimpanzee)	NEDD4L	XP_001140979.1
Canis lupus familiaris (dog)	NEDD4L	XP_005615425.1
Bos taurus (cattle)	NEDD4L	XP_005224408.1
Schizosaccharomyces pombe (fission yeast)	pub1	NP_594396.1
	NEDD4L	XP_001088661.2
Gallus gallus (chicken)	NEDD4L	XP_004949055.1
Eremothecium gossypii	AGOS_ADL055C	NP_984041.1
Neurospora crassa	NCU03947	XP_957586.2
Homo sapiens (human)	NEDD4L	NP_001138439.1
Drosophila melanogaster (fruit fly)	Nedd4	NP_730284.2
Xenopus tropicalis (tropical clawed frog)	nedd4l	NP_001006727.1
Caenorhabditis elegans (roundworm)	Y92H12A.2	NP_490865.3
Magnaporthe oryzae (rice blast fungus)	MGG_07255	XP_003715474.1
Mus musculus (house mouse)	Nedd4l	NP_001107858.1
Rattus norvegicus (Norway rat)	Nedd4l	NP_001008301.2
Danio rerio (zebrafish)	nedd4l	XP_005161145.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000787	XP_311327.5
Saccharomyces cerevisiae (baker's yeast)	RSP5	NP_011051.3
Kluyveromyces lactis	KLLA0E13575g	XP_454562.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

nedd4l cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

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