Nedd4 cDNA ORF clone, Drosophila melanogaster(fruit fly)

The following Nedd4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nedd4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OFa21017 NM_168737.2
Latest version!
Drosophila melanogaster Nedd4, transcript variant F (Nedd4), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OFa21018 NM_206394.2
Latest version!
Drosophila melanogaster Nedd4, transcript variant E (Nedd4), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OFa18832 NM_001275061.1
Latest version!
Drosophila melanogaster Nedd4, transcript variant L (Nedd4), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OFa18833 NM_001144492.2
Latest version!
Drosophila melanogaster Nedd4, transcript variant I (Nedd4), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OFa18834 NM_001144493.3
Latest version!
Drosophila melanogaster Nedd4, transcript variant H (Nedd4), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OFa18835 NM_001275062.1
Latest version!
Drosophila melanogaster Nedd4, transcript variant M (Nedd4), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OFa21013 NM_140736.3
Latest version!
Drosophila melanogaster Nedd4, transcript variant J (Nedd4), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OFa21014 NM_168738.4
Latest version!
Drosophila melanogaster Nedd4, transcript variant G (Nedd4), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OFa21015 NM_168736.4
Latest version!
Drosophila melanogaster Nedd4, transcript variant K (Nedd4), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OFa21017
    Clone ID Related Accession (Same CDS sequence) NM_168737.2
    Accession Version NM_168737.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2871bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product Nedd4, isoform F
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_168737.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGCCGAGT CAACAACCAC ATCGCCATCG GTGACGAGCG AAGATGGCCA AATCCATGGC 
    TGCAATAACA GTGATAACGA CTCTGGGGAC TCGTGTCACC TGCGAATCGT CGTACTCACC
    GGCCAGTCGC TGGCCAAAAA GGATATATTC GGCGCCAGCG ACCCGTATGT CAGAATCGAT
    TTGAACACAA TCAACGGTGA TATTAATATT GACTCTGTTT TGACGAAGAC CAAAAAGAAG
    ACACTGAATC CAACCTGGAA TGAAGAGTTC ATATTTAGAG TTAAACCGTC TGAGCATAAG
    CTTGTGTTTC AAGTATTTGA CGAGAATCGC CTGACACGCG ACGACTTCCT GGGCATGGTG
    GAGCTGACCC TGGTGAATCT GCCTACCGAG CAGGAAGGTC GCACCATCGG AGAACAAAGC
    TACACTCTGC GCCCGCGCAG GTCAGTAGGC GCCAAATCCC GCATCAAGGG CACACTGCGC
    ATCTACCACG CCTTTATCCG GGAGACGCGG GAGCAGAGCG AGCCATCTAG TGGCAATAGC
    GATGGCGAGT GGGAGCACGT TGAGGCCACC AATGCCGGTG AGACGTCTGC CCAGCCGCAC
    CCCTTTCCTA CTGGTGGCCA CGATGCCCTA CCCGCTGGAT GGGAAGAACG CCAGGATGCC
    AACGGGCGCA CGTACTATGT GAATCACACG GCGAGGACGA CGCAATGGGA TAGGCCTACT
    GTCTTAAACA GCCACAGTAG CCAATCCACT GACGACCAGT TGGCCTCGGA TTTCCAGAGA
    CGTTTCCACA TCAGCGTGGA CGACACGGAG TCAGGACGTT CGGCGGACTC CATCAGTCAC
    AATAGTATAG AGGACAATAA CAATGCTGCT GGCTTAGCAT ATACTCCAAA AACAGCAGCC
    ACCTCATCGG CACCACCCAA CACCCCAACA AACAACAATG GAATTCTCGC ACAAATTGCC
    ATGCAATATC GCGCAGAGGA AGATCAAGAT CCAACAGTGG ACCATACCAG GCAGCCCGAA
    ATCTCAGCGA CCAGTTTGCA GAACGACCTG CGGCCGGTAC GGGAGGCTCC CGGTGTGCCG
    GATATAGCCA TAACCAACCC GTTCACGCGG CGGGCCGCGG GCAATATGGC CGGCGGTGCG
    GGATGGCAGC AAGAACGGCG GCGACAGCAA ATGCAACTGC ATATCCAGCA GCATCAGCAG
    CGGCAACAGC AACAACAGCA GAATAGAATA TTGCTTGATG TGGATCATCG TCAGCAGGAG
    CCGCAACATC GGGGTCAGAG ACACCAGCAG CAACATCGGC CATCGAATGA AGACACCGAT
    CACACAGACA GCCACAATCC CTCTGACATC TCAGCTCCGT CCACACGACG CAATTCCGAG
    GAAGACAACG CGGCTGTGCC TCCCATGGAA CAAAATACTG GTGGTGAAGA GGAACCCCTG
    CCACCGCGCT GGTCGATGCA GGTAGCCCCC AACGGAAGGA CATTCTTCAT TGATCATGCG
    TCCCGGAGGA CTACTTGGAT AGACCCGCGC AACGGACGGG CCAGCCCTAT GCCAAATCAA
    ACTCGTCGCG TCGAGGACGA CCTGGGTCCT TTACCAGAGG GCTGGGAGGA GCGAGTACAC
    ACCGACGGAC GCGTATTCTA TATAGATCAT AACACGCGAA CTACGCAGTG GGAAGATCCT
    CGCTTGTCTA ATCCAAATAT TGCCGGTCAG GCTGTGCCCT ATTCTCGAGA TTACAAGCAA
    AAATACGAGT ATTTCAAGAG TCACATTAGA AAGCCTACAA ATGTACCAAA TAAATTTGAA
    ATACGTATTC GTCGTACGTC GATACTGGAG GATTCTTACA GAATTATTAG TTCGGTAACG
    AAGACCGATT TACTAAAGAC TAAATTATGG GTAGAGTTTG AAGGAGAAAC TGGCTTAGAT
    TATGGAGGCC TCGCAAGGGA ATGGTTCTAT TTATTATCAA AAGAAATGTT TAATCCGTAC
    TACGGACTAT TCGAGTACTC GGCCATGGAC AACTACACGC TTCAGATAAA CAATGGTAGT
    GGTTTGTGCA ACGAGGAGCA TTTAAGTTAC TTTAAATTCA TTGGCCGTAT TGCGGGCATG
    GCTGTGTATC ATGGAAAGCT GCTAGATGCC TTCTTCATTC GTCCATTCTA CAAGATGATG
    CTGCAGAAGC CGATTGACCT AAAGGACATG GAGTCTGTGG ACACGGAGTA CTACAACTCG
    CTGATGTGGA TTAAGGAGAA CGATCCACGC ATTCTGGAAC TGACTTTCTG CCTGGATGAA
    GACGTTTTTG GCCAGAAGAG TCAGCACGAA CTAAAGCCCG GCGGTGCCAA CATAGACGTG
    ACTAACGAAA ACAAGGATGA ATACATTAAA TTGGTTATCG AATGGCGCTT TGTGGCACGT
    GTCAAGGAAC AGATGTCGTC CTTCCTTGAT GGTTTTGGAT CGATAATTCC TCTGAATCTT
    ATTAAGATTT TCGACGAGCA CGAACTGGAG CTACTGATGT GTGGCATACA GAACATCGAT
    GTGAAGGATT GGCGCGAGAA TACTCTGTAC AAGGGCGACT ACCACATGAA TCATATTATT
    ATTCAGTGGT TCTGGAGGGC TGTACTTTCC TTCTCCAACG AGATGCGTTC TCGTCTGCTG
    CAATTTGTTA CGGGCACTTC ACGCGTGCCC ATGAACGGTT TCAAGGAGCT GTACGGCTCA
    AATGGCCCCC AGATGTTTAC TATCGAAAAG TGGGGCACTC CTAATAACTT TCCAAGGGCA
    CATACCTGTT TCAATCGCCT GGACCTGCCA CCCTATGAAG GCTACCTGCA ACTGAAGGAC
    AAACTGATCA AGGCCATCGA GGGCAGCCAA GGATTTGCTG GAGTTGATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_730283.1
    CDS281..3151
    Translation

    Target ORF information:

    RefSeq Version NM_168737.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster Nedd4, transcript variant F (Nedd4), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_168737.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGCCGAGT CAACAACCAC ATCGCCATCG GTGACGAGCG AAGATGGCCA AATCCATGGC 
    TGCAATAACA GTGATAACGA CTCTGGGGAC TCGTGTCACC TGCGAATCGT CGTACTCACC
    GGCCAGTCGC TGGCCAAAAA GGATATATTC GGCGCCAGCG ACCCGTATGT CAGAATCGAT
    TTGAACACAA TCAACGGTGA TATTAATATT GACTCTGTTT TGACGAAGAC CAAAAAGAAG
    ACACTGAATC CAACCTGGAA TGAAGAGTTC ATATTTAGAG TTAAACCGTC TGAGCATAAG
    CTTGTGTTTC AAGTATTTGA CGAGAATCGC CTGACACGCG ACGACTTCCT GGGCATGGTG
    GAGCTGACCC TGGTGAATCT GCCTACCGAG CAGGAAGGTC GCACCATCGG AGAACAAAGC
    TACACTCTGC GCCCGCGCAG GTCAGTAGGC GCCAAATCCC GCATCAAGGG CACACTGCGC
    ATCTACCACG CCTTTATCCG GGAGACGCGG GAGCAGAGCG AGCCATCTAG TGGCAATAGC
    GATGGCGAGT GGGAGCACGT TGAGGCCACC AATGCCGGTG AGACGTCTGC CCAGCCGCAC
    CCCTTTCCTA CTGGTGGCCA CGATGCCCTA CCCGCTGGAT GGGAAGAACG CCAGGATGCC
    AACGGGCGCA CGTACTATGT GAATCACACG GCGAGGACGA CGCAATGGGA TAGGCCTACT
    GTCTTAAACA GCCACAGTAG CCAATCCACT GACGACCAGT TGGCCTCGGA TTTCCAGAGA
    CGTTTCCACA TCAGCGTGGA CGACACGGAG TCAGGACGTT CGGCGGACTC CATCAGTCAC
    AATAGTATAG AGGACAATAA CAATGCTGCT GGCTTAGCAT ATACTCCAAA AACAGCAGCC
    ACCTCATCGG CACCACCCAA CACCCCAACA AACAACAATG GAATTCTCGC ACAAATTGCC
    ATGCAATATC GCGCAGAGGA AGATCAAGAT CCAACAGTGG ACCATACCAG GCAGCCCGAA
    ATCTCAGCGA CCAGTTTGCA GAACGACCTG CGGCCGGTAC GGGAGGCTCC CGGTGTGCCG
    GATATAGCCA TAACCAACCC GTTCACGCGG CGGGCCGCGG GCAATATGGC CGGCGGTGCG
    GGATGGCAGC AAGAACGGCG GCGACAGCAA ATGCAACTGC ATATCCAGCA GCATCAGCAG
    CGGCAACAGC AACAACAGCA GAATAGAATA TTGCTTGATG TGGATCATCG TCAGCAGGAG
    CCGCAACATC GGGGTCAGAG ACACCAGCAG CAACATCGGC CATCGAATGA AGACACCGAT
    CACACAGACA GCCACAATCC CTCTGACATC TCAGCTCCGT CCACACGACG CAATTCCGAG
    GAAGACAACG CGGCTGTGCC TCCCATGGAA CAAAATACTG GTGGTGAAGA GGAACCCCTG
    CCACCGCGCT GGTCGATGCA GGTAGCCCCC AACGGAAGGA CATTCTTCAT TGATCATGCG
    TCCCGGAGGA CTACTTGGAT AGACCCGCGC AACGGACGGG CCAGCCCTAT GCCAAATCAA
    ACTCGTCGCG TCGAGGACGA CCTGGGTCCT TTACCAGAGG GCTGGGAGGA GCGAGTACAC
    ACCGACGGAC GCGTATTCTA TATAGATCAT AACACGCGAA CTACGCAGTG GGAAGATCCT
    CGCTTGTCTA ATCCAAATAT TGCCGGTCAG GCTGTGCCCT ATTCTCGAGA TTACAAGCAA
    AAATACGAGT ATTTCAAGAG TCACATTAGA AAGCCTACAA ATGTACCAAA TAAATTTGAA
    ATACGTATTC GTCGTACGTC GATACTGGAG GATTCTTACA GAATTATTAG TTCGGTAACG
    AAGACCGATT TACTAAAGAC TAAATTATGG GTAGAGTTTG AAGGAGAAAC TGGCTTAGAT
    TATGGAGGCC TCGCAAGGGA ATGGTTCTAT TTATTATCAA AAGAAATGTT TAATCCGTAC
    TACGGACTAT TCGAGTACTC GGCCATGGAC AACTACACGC TTCAGATAAA CAATGGTAGT
    GGTTTGTGCA ACGAGGAGCA TTTAAGTTAC TTTAAATTCA TTGGCCGTAT TGCGGGCATG
    GCTGTGTATC ATGGAAAGCT GCTAGATGCC TTCTTCATTC GTCCATTCTA CAAGATGATG
    CTGCAGAAGC CGATTGACCT AAAGGACATG GAGTCTGTGG ACACGGAGTA CTACAACTCG
    CTGATGTGGA TTAAGGAGAA CGATCCACGC ATTCTGGAAC TGACTTTCTG CCTGGATGAA
    GACGTTTTTG GCCAGAAGAG TCAGCACGAA CTAAAGCCCG GCGGTGCCAA CATAGACGTG
    ACTAACGAAA ACAAGGATGA ATACATTAAA TTGGTTATCG AATGGCGCTT TGTGGCACGT
    GTCAAGGAAC AGATGTCGTC CTTCCTTGAT GGTTTTGGAT CGATAATTCC TCTGAATCTT
    ATTAAGATTT TCGACGAGCA CGAACTGGAG CTACTGATGT GTGGCATACA GAACATCGAT
    GTGAAGGATT GGCGCGAGAA TACTCTGTAC AAGGGCGACT ACCACATGAA TCATATTATT
    ATTCAGTGGT TCTGGAGGGC TGTACTTTCC TTCTCCAACG AGATGCGTTC TCGTCTGCTG
    CAATTTGTTA CGGGCACTTC ACGCGTGCCC ATGAACGGTT TCAAGGAGCT GTACGGCTCA
    AATGGCCCCC AGATGTTTAC TATCGAAAAG TGGGGCACTC CTAATAACTT TCCAAGGGCA
    CATACCTGTT TCAATCGCCT GGACCTGCCA CCCTATGAAG GCTACCTGCA ACTGAAGGAC
    AAACTGATCA AGGCCATCGA GGGCAGCCAA GGATTTGCTG GAGTTGATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa21018
    Clone ID Related Accession (Same CDS sequence) NM_206394.2
    Accession Version NM_206394.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2382bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product Nedd4, isoform E
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_206394.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGCCGAGT CAACAACCAC ATCGCCATCG GTGACGAGCG AAGATGGCCA AATCCATGGC 
    TGCAATAACA GTGATAACGA CTCTGGGGAC TCGTGTCACC TGCGAATCGT CGTACTCACC
    GGCCAGTCGC TGGCCAAAAA GGATATATTC GGCGCCAGCG ACCCGTATGT CAGAATCGAT
    TTGAACACAA TCAACGGTGA TATTAATATT GACTCTGTTT TGACGAAGAC CAAAAAGAAG
    ACACTGAATC CAACCTGGAA TGAAGAGTTC ATATTTAGAG TTAAACCGTC TGAGCATAAG
    CTTGTGTTTC AAGTATTTGA CGAGAATCGC CTGACACGCG ACGACTTCCT GGGCATGGTG
    GAGCTGACCC TGGTGAATCT GCCTACCGAG CAGGAAGGTC GCACCATCGG AGAACAAAGC
    TACACTCTGC GCCCGCGCAG CGCCAAATCC CGCATCAAGG GCACACTGCG CATCTACCAC
    GCCTTTATCC GGGAGACGCG GGAGCAGAGC GAGCCATCTA GTGGCAATAG CGATGGCGAG
    TGGGAGCACG TTGAGGCCAC CAATGCCGGT GAGACGTCTG CCCAGCCGCA CCCCTTTCCT
    ACTGGTGGCC ACGATGCCCT ACCCGCTGGA TGGGAAGAAC GCCAGGATGC CAACGGGCGC
    ACGTACTATG TGAATCACAC GGCGAGGACG ACGCAATGGG ATAGGCCTAC TGTCTTAAAC
    AGCCACAGTA GCCAATCCAC TGACGACCAG TTGGCCTCGG ATTTCCAGAG ACGTTTCCAC
    ATCAGCGTGG ACGACACGGA GTCAGGACGT TCGGCGAATG AAGACACCGA TCACACAGAC
    AGCCACAATC CCTCTGACAT CTCAGCTCCG TCCACACGAC GCAATTCCGA GGAAGACAAC
    GCGGCTGTGC CTCCCATGGA ACAAAATACT GGTGGTGAAG AGGAACCCCT GCCACCGCGC
    TGGTCGATGC AGGTAGCCCC CAACGGAAGG ACATTCTTCA TTGATCATGC GTCCCGGAGG
    ACTACTTGGA TAGACCCGCG CAACGGACGG GCCAGCCCTA TGCCAAATCA AACTCGTCGC
    GTCGAGGACG ACCTGGGTCC TTTACCAGAG GGCTGGGAGG AGCGAGTACA CACCGACGGA
    CGCGTATTCT ATATAGATCA TAACACGCGA ACTACGCAGT GGGAAGATCC TCGCTTGTCT
    AATCCAAATA TTGCCGGTCA GGCTGTGCCC TATTCTCGAG ATTACAAGCA AAAATACGAG
    TATTTCAAGA GTCACATTAG AAAGCCTACA AATGTACCAA ATAAATTTGA AATACGTATT
    CGTCGTACGT CGATACTGGA GGATTCTTAC AGAATTATTA GTTCGGTAAC GAAGACCGAT
    TTACTAAAGA CTAAATTATG GGTAGAGTTT GAAGGAGAAA CTGGCTTAGA TTATGGAGGC
    CTCGCAAGGG AATGGTTCTA TTTATTATCA AAAGAAATGT TTAATCCGTA CTACGGACTA
    TTCGAGTACT CGGCCATGGA CAACTACACG CTTCAGATAA ACAATGGTAG TGGTTTGTGC
    AACGAGGAGC ATTTAAGTTA CTTTAAATTC ATTGGCCGTA TTGCGGGCAT GGCTGTGTAT
    CATGGAAAGC TGCTAGATGC CTTCTTCATT CGTCCATTCT ACAAGATGAT GCTGCAGAAG
    CCGATTGACC TAAAGGACAT GGAGTCTGTG GACACGGAGT ACTACAACTC GCTGATGTGG
    ATTAAGGAGA ACGATCCACG CATTCTGGAA CTGACTTTCT GCCTGGATGA AGACGTTTTT
    GGCCAGAAGA GTCAGCACGA ACTAAAGCCC GGCGGTGCCA ACATAGACGT GACTAACGAA
    AACAAGGATG AATACATTAA ATTGGTTATC GAATGGCGCT TTGTGGCACG TGTCAAGGAA
    CAGATGTCGT CCTTCCTTGA TGGTTTTGGA TCGATAATTC CTCTGAATCT TATTAAGATT
    TTCGACGAGC ACGAACTGGA GCTACTGATG TGTGGCATAC AGAACATCGA TGTGAAGGAT
    TGGCGCGAGA ATACTCTGTA CAAGGGCGAC TACCACATGA ATCATATTAT TATTCAGTGG
    TTCTGGAGGG CTGTACTTTC CTTCTCCAAC GAGATGCGTT CTCGTCTGCT GCAATTTGTT
    ACGGGCACTT CACGCGTGCC CATGAACGGT TTCAAGGAGC TGTACGGCTC AAATGGCCCC
    CAGATGTTTA CTATCGAAAA GTGGGGCACT CCTAATAACT TTCCAAGGGC ACATACCTGT
    TTCAATCGCC TGGACCTGCC ACCCTATGAA GGCTACCTGC AACTGAAGGA CAAACTGATC
    AAGGCCATCG AGGGCAGCCA AGGATTTGCT GGAGTTGATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_996116.1
    CDS237..2618
    Translation

    Target ORF information:

    RefSeq Version NM_206394.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster Nedd4, transcript variant E (Nedd4), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_206394.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGCCGAGT CAACAACCAC ATCGCCATCG GTGACGAGCG AAGATGGCCA AATCCATGGC 
    TGCAATAACA GTGATAACGA CTCTGGGGAC TCGTGTCACC TGCGAATCGT CGTACTCACC
    GGCCAGTCGC TGGCCAAAAA GGATATATTC GGCGCCAGCG ACCCGTATGT CAGAATCGAT
    TTGAACACAA TCAACGGTGA TATTAATATT GACTCTGTTT TGACGAAGAC CAAAAAGAAG
    ACACTGAATC CAACCTGGAA TGAAGAGTTC ATATTTAGAG TTAAACCGTC TGAGCATAAG
    CTTGTGTTTC AAGTATTTGA CGAGAATCGC CTGACACGCG ACGACTTCCT GGGCATGGTG
    GAGCTGACCC TGGTGAATCT GCCTACCGAG CAGGAAGGTC GCACCATCGG AGAACAAAGC
    TACACTCTGC GCCCGCGCAG CGCCAAATCC CGCATCAAGG GCACACTGCG CATCTACCAC
    GCCTTTATCC GGGAGACGCG GGAGCAGAGC GAGCCATCTA GTGGCAATAG CGATGGCGAG
    TGGGAGCACG TTGAGGCCAC CAATGCCGGT GAGACGTCTG CCCAGCCGCA CCCCTTTCCT
    ACTGGTGGCC ACGATGCCCT ACCCGCTGGA TGGGAAGAAC GCCAGGATGC CAACGGGCGC
    ACGTACTATG TGAATCACAC GGCGAGGACG ACGCAATGGG ATAGGCCTAC TGTCTTAAAC
    AGCCACAGTA GCCAATCCAC TGACGACCAG TTGGCCTCGG ATTTCCAGAG ACGTTTCCAC
    ATCAGCGTGG ACGACACGGA GTCAGGACGT TCGGCGAATG AAGACACCGA TCACACAGAC
    AGCCACAATC CCTCTGACAT CTCAGCTCCG TCCACACGAC GCAATTCCGA GGAAGACAAC
    GCGGCTGTGC CTCCCATGGA ACAAAATACT GGTGGTGAAG AGGAACCCCT GCCACCGCGC
    TGGTCGATGC AGGTAGCCCC CAACGGAAGG ACATTCTTCA TTGATCATGC GTCCCGGAGG
    ACTACTTGGA TAGACCCGCG CAACGGACGG GCCAGCCCTA TGCCAAATCA AACTCGTCGC
    GTCGAGGACG ACCTGGGTCC TTTACCAGAG GGCTGGGAGG AGCGAGTACA CACCGACGGA
    CGCGTATTCT ATATAGATCA TAACACGCGA ACTACGCAGT GGGAAGATCC TCGCTTGTCT
    AATCCAAATA TTGCCGGTCA GGCTGTGCCC TATTCTCGAG ATTACAAGCA AAAATACGAG
    TATTTCAAGA GTCACATTAG AAAGCCTACA AATGTACCAA ATAAATTTGA AATACGTATT
    CGTCGTACGT CGATACTGGA GGATTCTTAC AGAATTATTA GTTCGGTAAC GAAGACCGAT
    TTACTAAAGA CTAAATTATG GGTAGAGTTT GAAGGAGAAA CTGGCTTAGA TTATGGAGGC
    CTCGCAAGGG AATGGTTCTA TTTATTATCA AAAGAAATGT TTAATCCGTA CTACGGACTA
    TTCGAGTACT CGGCCATGGA CAACTACACG CTTCAGATAA ACAATGGTAG TGGTTTGTGC
    AACGAGGAGC ATTTAAGTTA CTTTAAATTC ATTGGCCGTA TTGCGGGCAT GGCTGTGTAT
    CATGGAAAGC TGCTAGATGC CTTCTTCATT CGTCCATTCT ACAAGATGAT GCTGCAGAAG
    CCGATTGACC TAAAGGACAT GGAGTCTGTG GACACGGAGT ACTACAACTC GCTGATGTGG
    ATTAAGGAGA ACGATCCACG CATTCTGGAA CTGACTTTCT GCCTGGATGA AGACGTTTTT
    GGCCAGAAGA GTCAGCACGA ACTAAAGCCC GGCGGTGCCA ACATAGACGT GACTAACGAA
    AACAAGGATG AATACATTAA ATTGGTTATC GAATGGCGCT TTGTGGCACG TGTCAAGGAA
    CAGATGTCGT CCTTCCTTGA TGGTTTTGGA TCGATAATTC CTCTGAATCT TATTAAGATT
    TTCGACGAGC ACGAACTGGA GCTACTGATG TGTGGCATAC AGAACATCGA TGTGAAGGAT
    TGGCGCGAGA ATACTCTGTA CAAGGGCGAC TACCACATGA ATCATATTAT TATTCAGTGG
    TTCTGGAGGG CTGTACTTTC CTTCTCCAAC GAGATGCGTT CTCGTCTGCT GCAATTTGTT
    ACGGGCACTT CACGCGTGCC CATGAACGGT TTCAAGGAGC TGTACGGCTC AAATGGCCCC
    CAGATGTTTA CTATCGAAAA GTGGGGCACT CCTAATAACT TTCCAAGGGC ACATACCTGT
    TTCAATCGCC TGGACCTGCC ACCCTATGAA GGCTACCTGC AACTGAAGGA CAAACTGATC
    AAGGCCATCG AGGGCAGCCA AGGATTTGCT GGAGTTGATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa18832
    Clone ID Related Accession (Same CDS sequence) NM_001275061.1
    Accession Version NM_001275061.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3018bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product Nedd4, isoform L
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGTCGGCAC GTTCCAGCGG CTTGGTGGCC GCGGCGGCCC TTCCCGTCCC GTCGTCTTCT 
    TCCTCGGTGG CCGGCGGCGA CGTGCCACGC CCGCCGCCCC GCCGGCGAGC GGCCTCTGTT
    GCGGGACAAC AGCAAACGCG CCAGGAATTT GGAAATGGTT ACACTCCTCG CAGATCGTTG
    GCAGCAGTGA ACGACTCTGG GGACTCGTGT CACCTGCGAA TCGTCGTACT CACCGGCCAG
    TCGCTGGCCA AAAAGGATAT ATTCGGCGCC AGCGACCCGT ATGTCAGAAT CGATTTGAAC
    ACAATCAACG GTGATATTAA TATTGACTCT GTTTTGACGA AGACCAAAAA GAAGACACTG
    AATCCAACCT GGAATGAAGA GTTCATATTT AGAGTTAAAC CGTCTGAGCA TAAGCTTGTG
    TTTCAAGTAT TTGACGAGAA TCGCCTGACA CGCGACGACT TCCTGGGCAT GGTGGAGCTG
    ACCCTGGTGA ATCTGCCTAC CGAGCAGGAA GGTCGCACCA TCGGAGAACA AAGCTACACT
    CTGCGCCCGC GCAGGTCAGT AGGCAGCGCC AAATCCCGCA TCAAGGGCAC ACTGCGCATC
    TACCACGCCT TTATCCGGGA GACGCGGGAG CAGAGCGAGC CATCTAGTGG CAATAGCGAT
    GGCGAGTGGG AGCACGTTGA GGCCACCAAT GCCGGTGAGA CGTCTGCCCA GCCGCACCCC
    TTTCCTACTG GTGGCCACGA TGCCCTACCC GCTGGATGGG AAGAACGCCA GGATGCCAAC
    GGGCGCACGT ACTATGTGAA TCACACGGCG AGGACGACGC AATGGGATAG GCCTACTGTC
    CACAGTAGCC AATCCACTGA CGACCAGTTG GCCTCGGATT TCCAGAGACG TTTCCACATC
    AGCGTGGACG ACACGGAGTC AGGACGTTCG GCGGACTCCA TCAGTCACAA TAGTATAGAG
    GACAATAACA ATGCTGCTGG CTTAGCATAT ACTCCAAAAA CAGCAGCCAC CTCATCGGCA
    CCACCCAACA CCCCAACAAA CAACAATGGA ATTCTCGCAC AAATTGCCAT GCAATATCGC
    GCAGAGGAAG ATCAAGATCC AACAGTGGAC CATACCAGTT TTGTTTACAA TTCCCTGCGA
    CATCCTGTCG CCCATAGGCA GCCCGAAATC TCAGCGACCA GTTTGCAGAA CGACCTGCGG
    CCGGTACGGG AGGCTCCCGG TGTGCCGGAT ATAGCCATAA CCAACCCGTT CACGCGGCGG
    GCCGCGGGCA ATATGGCCGG CGGTGCGGGA TGGCAGCAAG AACGGCGGCG ACAGCAAATG
    CAACTGCATA TCCAGCAGCA TCAGCAGCGG CAACAGCAAC AACAGCAGAA TAGAATATTG
    CTTGATGTGG ATCATCGTCA GCAGGAGCCG CAACATCGGG GTCAGAGACA CCAGCAGCAA
    CATCGGCCAT CGAATGAAGA CACCGATCAC ACAGACAGCC ACAATCCCTC TGACATCTCA
    GCTCCGTCCA CACGACGCAA TTCCGAGGAA GACAACGCGG CTGTGCCTCC CATGGAACAA
    AATACTGGTG GTGAAGAGGA ACCCCTGCCA CCGCGCTGGT CGATGCAGGT AGCCCCCAAC
    GGAAGGACAT TCTTCATTGA TCATGCGTCC CGGAGGACTA CTTGGATAGA CCCGCGCAAC
    GGACGGGCCA GCCCTATGCC AAATCAAACT CGTCGCGTCG AGGACGACCT GGGTCCTTTA
    CCAGAGGGCT GGGAGGAGCG AGTACACACC GACGGACGCG TATTCTATAT AGATCATAAC
    ACGCGAACTA CGCAGTGGGA AGATCCTCGC TTGTCTAATC CAAATATTGC CGGTCAGGCT
    GTGCCCTATT CTCGAGATTA CAAGCAAAAA TACGAGTATT TCAAGAGTCA CATTAGAAAG
    CCTACAAATG TACCAAATAA ATTTGAAATA CGTATTCGTC GTACGTCGAT ACTGGAGGAT
    TCTTACAGAA TTATTAGTTC GGTAACGAAG ACCGATTTAC TAAAGACTAA ATTATGGGTA
    GAGTTTGAAG GAGAAACTGG CTTAGATTAT GGAGGCCTCG CAAGGGAATG GTTCTATTTA
    TTATCAAAAG AAATGTTTAA TCCGTACTAC GGACTATTCG AGTACTCGGC CATGGACAAC
    TACACGCTTC AGATAAACAA TGGTAGTGGT TTGTGCAACG AGGAGCATTT AAGTTACTTT
    AAATTCATTG GCCGTATTGC GGGCATGGCT GTGTATCATG GAAAGCTGCT AGATGCCTTC
    TTCATTCGTC CATTCTACAA GATGATGCTG CAGAAGCCGA TTGACCTAAA GGACATGGAG
    TCTGTGGACA CGGAGTACTA CAACTCGCTG ATGTGGATTA AGGAGAACGA TCCACGCATT
    CTGGAACTGA CTTTCTGCCT GGATGAAGAC GTTTTTGGCC AGAAGAGTCA GCACGAACTA
    AAGCCCGGCG GTGCCAACAT AGACGTGACT AACGAAAACA AGGATGAATA CATTAAATTG
    GTTATCGAAT GGCGCTTTGT GGCACGTGTC AAGGAACAGA TGTCGTCCTT CCTTGATGGT
    TTTGGATCGA TAATTCCTCT GAATCTTATT AAGATTTTCG ACGAGCACGA ACTGGAGCTA
    CTGATGTGTG GCATACAGAA CATCGATGTG AAGGATTGGC GCGAGAATAC TCTGTACAAG
    GGCGACTACC ACATGAATCA TATTATTATT CAGTGGTTCT GGAGGGCTGT ACTTTCCTTC
    TCCAACGAGA TGCGTTCTCG TCTGCTGCAA TTTGTTACGG GCACTTCACG CGTGCCCATG
    AACGGTTTCA AGGAGCTGTA CGGCTCAAAT GGCCCCCAGA TGTTTACTAT CGAAAAGTGG
    GGCACTCCTA ATAACTTTCC AAGGGCACAT ACCTGTTTCA ATCGCCTGGA CCTGCCACCC
    TATGAAGGCT ACCTGCAACT GAAGGACAAA CTGATCAAGG CCATCGAGGG CAGCCAAGGA
    TTTGCTGGAG TTGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001261990.1
    CDS381..3398
    Translation

    Target ORF information:

    RefSeq Version NM_001275061.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster Nedd4, transcript variant L (Nedd4), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001275061.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGTCGGCAC GTTCCAGCGG CTTGGTGGCC GCGGCGGCCC TTCCCGTCCC GTCGTCTTCT 
    TCCTCGGTGG CCGGCGGCGA CGTGCCACGC CCGCCGCCCC GCCGGCGAGC GGCCTCTGTT
    GCGGGACAAC AGCAAACGCG CCAGGAATTT GGAAATGGTT ACACTCCTCG CAGATCGTTG
    GCAGCAGTGA ACGACTCTGG GGACTCGTGT CACCTGCGAA TCGTCGTACT CACCGGCCAG
    TCGCTGGCCA AAAAGGATAT ATTCGGCGCC AGCGACCCGT ATGTCAGAAT CGATTTGAAC
    ACAATCAACG GTGATATTAA TATTGACTCT GTTTTGACGA AGACCAAAAA GAAGACACTG
    AATCCAACCT GGAATGAAGA GTTCATATTT AGAGTTAAAC CGTCTGAGCA TAAGCTTGTG
    TTTCAAGTAT TTGACGAGAA TCGCCTGACA CGCGACGACT TCCTGGGCAT GGTGGAGCTG
    ACCCTGGTGA ATCTGCCTAC CGAGCAGGAA GGTCGCACCA TCGGAGAACA AAGCTACACT
    CTGCGCCCGC GCAGGTCAGT AGGCAGCGCC AAATCCCGCA TCAAGGGCAC ACTGCGCATC
    TACCACGCCT TTATCCGGGA GACGCGGGAG CAGAGCGAGC CATCTAGTGG CAATAGCGAT
    GGCGAGTGGG AGCACGTTGA GGCCACCAAT GCCGGTGAGA CGTCTGCCCA GCCGCACCCC
    TTTCCTACTG GTGGCCACGA TGCCCTACCC GCTGGATGGG AAGAACGCCA GGATGCCAAC
    GGGCGCACGT ACTATGTGAA TCACACGGCG AGGACGACGC AATGGGATAG GCCTACTGTC
    CACAGTAGCC AATCCACTGA CGACCAGTTG GCCTCGGATT TCCAGAGACG TTTCCACATC
    AGCGTGGACG ACACGGAGTC AGGACGTTCG GCGGACTCCA TCAGTCACAA TAGTATAGAG
    GACAATAACA ATGCTGCTGG CTTAGCATAT ACTCCAAAAA CAGCAGCCAC CTCATCGGCA
    CCACCCAACA CCCCAACAAA CAACAATGGA ATTCTCGCAC AAATTGCCAT GCAATATCGC
    GCAGAGGAAG ATCAAGATCC AACAGTGGAC CATACCAGTT TTGTTTACAA TTCCCTGCGA
    CATCCTGTCG CCCATAGGCA GCCCGAAATC TCAGCGACCA GTTTGCAGAA CGACCTGCGG
    CCGGTACGGG AGGCTCCCGG TGTGCCGGAT ATAGCCATAA CCAACCCGTT CACGCGGCGG
    GCCGCGGGCA ATATGGCCGG CGGTGCGGGA TGGCAGCAAG AACGGCGGCG ACAGCAAATG
    CAACTGCATA TCCAGCAGCA TCAGCAGCGG CAACAGCAAC AACAGCAGAA TAGAATATTG
    CTTGATGTGG ATCATCGTCA GCAGGAGCCG CAACATCGGG GTCAGAGACA CCAGCAGCAA
    CATCGGCCAT CGAATGAAGA CACCGATCAC ACAGACAGCC ACAATCCCTC TGACATCTCA
    GCTCCGTCCA CACGACGCAA TTCCGAGGAA GACAACGCGG CTGTGCCTCC CATGGAACAA
    AATACTGGTG GTGAAGAGGA ACCCCTGCCA CCGCGCTGGT CGATGCAGGT AGCCCCCAAC
    GGAAGGACAT TCTTCATTGA TCATGCGTCC CGGAGGACTA CTTGGATAGA CCCGCGCAAC
    GGACGGGCCA GCCCTATGCC AAATCAAACT CGTCGCGTCG AGGACGACCT GGGTCCTTTA
    CCAGAGGGCT GGGAGGAGCG AGTACACACC GACGGACGCG TATTCTATAT AGATCATAAC
    ACGCGAACTA CGCAGTGGGA AGATCCTCGC TTGTCTAATC CAAATATTGC CGGTCAGGCT
    GTGCCCTATT CTCGAGATTA CAAGCAAAAA TACGAGTATT TCAAGAGTCA CATTAGAAAG
    CCTACAAATG TACCAAATAA ATTTGAAATA CGTATTCGTC GTACGTCGAT ACTGGAGGAT
    TCTTACAGAA TTATTAGTTC GGTAACGAAG ACCGATTTAC TAAAGACTAA ATTATGGGTA
    GAGTTTGAAG GAGAAACTGG CTTAGATTAT GGAGGCCTCG CAAGGGAATG GTTCTATTTA
    TTATCAAAAG AAATGTTTAA TCCGTACTAC GGACTATTCG AGTACTCGGC CATGGACAAC
    TACACGCTTC AGATAAACAA TGGTAGTGGT TTGTGCAACG AGGAGCATTT AAGTTACTTT
    AAATTCATTG GCCGTATTGC GGGCATGGCT GTGTATCATG GAAAGCTGCT AGATGCCTTC
    TTCATTCGTC CATTCTACAA GATGATGCTG CAGAAGCCGA TTGACCTAAA GGACATGGAG
    TCTGTGGACA CGGAGTACTA CAACTCGCTG ATGTGGATTA AGGAGAACGA TCCACGCATT
    CTGGAACTGA CTTTCTGCCT GGATGAAGAC GTTTTTGGCC AGAAGAGTCA GCACGAACTA
    AAGCCCGGCG GTGCCAACAT AGACGTGACT AACGAAAACA AGGATGAATA CATTAAATTG
    GTTATCGAAT GGCGCTTTGT GGCACGTGTC AAGGAACAGA TGTCGTCCTT CCTTGATGGT
    TTTGGATCGA TAATTCCTCT GAATCTTATT AAGATTTTCG ACGAGCACGA ACTGGAGCTA
    CTGATGTGTG GCATACAGAA CATCGATGTG AAGGATTGGC GCGAGAATAC TCTGTACAAG
    GGCGACTACC ACATGAATCA TATTATTATT CAGTGGTTCT GGAGGGCTGT ACTTTCCTTC
    TCCAACGAGA TGCGTTCTCG TCTGCTGCAA TTTGTTACGG GCACTTCACG CGTGCCCATG
    AACGGTTTCA AGGAGCTGTA CGGCTCAAAT GGCCCCCAGA TGTTTACTAT CGAAAAGTGG
    GGCACTCCTA ATAACTTTCC AAGGGCACAT ACCTGTTTCA ATCGCCTGGA CCTGCCACCC
    TATGAAGGCT ACCTGCAACT GAAGGACAAA CTGATCAAGG CCATCGAGGG CAGCCAAGGA
    TTTGCTGGAG TTGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa18833
    Clone ID Related Accession (Same CDS sequence) NM_001144492.2
    Accession Version NM_001144492.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2952bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product Nedd4, isoform I
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_001144492.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTCGGCAC GTTCCAGCGG CTTGGTGGCC GCGGCGGCCC TTCCCGTCCC GTCGTCTTCT 
    TCCTCGGTGG CCGGCGGCGA CGTGCCACGC CCGCCGCCCC GCCGGCGAGC GGCCTCTGTT
    GCGGGACAAC AGCAAACGCG CCAGGAATTT GGAAATGGTT ACACTCCTCG CAGATCGTTG
    GCAGCAGTGA ACGACTCTGG GGACTCGTGT CACCTGCGAA TCGTCGTACT CACCGGCCAG
    TCGCTGGCCA AAAAGGATAT ATTCGGCGCC AGCGACCCGT ATGTCAGAAT CGATTTGAAC
    ACAATCAACG GTGATATTAA TATTGACTCT GTTTTGACGA AGACCAAAAA GAAGACACTG
    AATCCAACCT GGAATGAAGA GTTCATATTT AGAGTTAAAC CGTCTGAGCA TAAGCTTGTG
    TTTCAAGTAT TTGACGAGAA TCGCCTGACA CGCGACGACT TCCTGGGCAT GGTGGAGCTG
    ACCCTGGTGA ATCTGCCTAC CGAGCAGGAA GGTCGCACCA TCGGAGAACA AAGCTACACT
    CTGCGCCCGC GCAGGTCAGT AGGCGCCAAA TCCCGCATCA AGGGCACACT GCGCATCTAC
    CACGCCTTTA TCCGGGAGAC GCGGGAGCAG AGCGAGCCAT CTAGTGGCAA TAGCGATGGC
    GAGTGGGAGC ACGTTGAGGC CACCAATGCC GGTGAGACGT CTGCCCAGCC GCACCCCTTT
    CCTACTGGTG GCCACGATGC CCTACCCGCT GGATGGGAAG AACGCCAGGA TGCCAACGGG
    CGCACGTACT ATGTGAATCA CACGGCGAGG ACGACGCAAT GGGATAGGCC TACTGTCTTA
    AACAGCCACA GTAGCCAATC CACTGACGAC CAGTTGGCCT CGGATTTCCA GAGACGTTTC
    CACATCAGCG TGGACGACAC GGAGTCAGGA CGTTCGGCGG ACTCCATCAG TCACAATAGT
    ATAGAGGACA ATAACAATGC TGCTGGCTTA GCATATACTC CAAAAACAGC AGCCACCTCA
    TCGGCACCAC CCAACACCCC AACAAACAAC AATGGAATTC TCGCACAAAT TGCCATGCAA
    TATCGCGCAG AGGAAGATCA AGATCCAACA GTGGACCATA CCAGTTTTGT TTACAATTCC
    CTGCGACATC CTGTCGCCCA TAGGCAGCCC GAAATCTCAG CGACCAGTTT GCAGAACGAC
    CTGCGGCCGG TACGGGAGGC TCCCGGTGTG CCGGATATAG CCATAACCAA CCCGTTCACG
    CGGCGGGCCG CGGGCAATAT GGCCGGCGGT GCGGGATGGC AGCAAGAACG GCGGCGACAG
    CAAATGCAAC TGCATATCCA GCAGCATCAG CAGCGGCAAC AGCAACAACA GCAGAATAGA
    ATATTGAATG AAGACACCGA TCACACAGAC AGCCACAATC CCTCTGACAT CTCAGCTCCG
    TCCACACGAC GCAATTCCGA GGAAGACAAC GCGGCTGTGC CTCCCATGGA ACAAAATACT
    GGTGGTGAAG AGGAACCCCT GCCACCGCGC TGGTCGATGC AGGTAGCCCC CAACGGAAGG
    ACATTCTTCA TTGATCATGC GTCCCGGAGG ACTACTTGGA TAGACCCGCG CAACGGACGG
    GCCAGCCCTA TGCCAAATCA AACTCGTCGC GTCGAGGACG ACCTGGGTCC TTTACCAGAG
    GGCTGGGAGG AGCGAGTACA CACCGACGGA CGCGTATTCT ATATAGATCA TAACACGCGA
    ACTACGCAGT GGGAAGATCC TCGCTTGTCT AATCCAAATA TTGCCGGTCA GGCTGTGCCC
    TATTCTCGAG ATTACAAGCA AAAATACGAG TATTTCAAGA GTCACATTAG AAAGCCTACA
    AATGTACCAA ATAAATTTGA AATACGTATT CGTCGTACGT CGATACTGGA GGATTCTTAC
    AGAATTATTA GTTCGGTAAC GAAGACCGAT TTACTAAAGA CTAAATTATG GGTAGAGTTT
    GAAGGAGAAA CTGGCTTAGA TTATGGAGGC CTCGCAAGGG AATGGTTCTA TTTATTATCA
    AAAGAAATGT TTAATCCGTA CTACGGACTA TTCGAGTACT CGGCCATGGA CAACTACACG
    CTTCAGATAA ACAATGGTAG TGGTTTGTGC AACGAGGAGC ATTTAAGTTA CTTTAAATTC
    ATTGGCCGTA TTGCGGGCAT GGCTGTGTAT CATGGAAAGC TGCTAGATGC CTTCTTCATT
    CGTCCATTCT ACAAGATGAT GCTGCAGAAG CCGATTGACC TAAAGGACAT GGAGTCTGTG
    GACACGGAGT ACTACAACTC GCTGATGTGG ATTAAGGAGA ACGATCCACG CATTCTGGAA
    CTGACTTTCT GCCTGGATGA AGACGTTTTT GGCCAGAAGA GTCAGCACGA ACTAAAGCCC
    GGCGGTGCCA ACATAGACGT GACTAACGAA AACAAGGATG AATACATTAA ATTGGTTATC
    GAATGGCGCT TTGTGGCACG TGTCAAGGAA CAGATGTCGT CCTTCCTTGA TGGTTTTGGA
    TCGATAATTC CTCTGAATCT TATTAAGATT TTCGACGAGC ACGAACTGGA GCTACTGATG
    TGTGGCATAC AGAACATCGA TGTGAAGGAT TGGCGCGAGA ATACTCTGTA CAAGGGCGAC
    TACCACATGA ATCATATTAT TATTCAGTGG TTCTGGAGGG CTGTACTTTC CTTCTCCAAC
    GAGATGCGTT CTCGTCTGCT GCAATTTGTT ACGGGCACTT CACGCGTGCC CATGAACGGT
    TTCAAGGAGC TGTACGGCTC AAATGGCCCC CAGATGTTTA CTATCGAAAA GTGGGGCACT
    CCTAATAACT TTCCAAGGGC ACATACCTGT TTCAATCGCC TGGACCTGCC ACCCTATGAA
    GGCTACCTGC AACTGAAGGA CAAACTGATC AAGGCCATCG AGGGCAGCCA AGGATTTGCT
    GGAGTTGATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001137964.1
    CDS381..3332
    Translation

    Target ORF information:

    RefSeq Version NM_001144492.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster Nedd4, transcript variant I (Nedd4), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001144492.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTCGGCAC GTTCCAGCGG CTTGGTGGCC GCGGCGGCCC TTCCCGTCCC GTCGTCTTCT 
    TCCTCGGTGG CCGGCGGCGA CGTGCCACGC CCGCCGCCCC GCCGGCGAGC GGCCTCTGTT
    GCGGGACAAC AGCAAACGCG CCAGGAATTT GGAAATGGTT ACACTCCTCG CAGATCGTTG
    GCAGCAGTGA ACGACTCTGG GGACTCGTGT CACCTGCGAA TCGTCGTACT CACCGGCCAG
    TCGCTGGCCA AAAAGGATAT ATTCGGCGCC AGCGACCCGT ATGTCAGAAT CGATTTGAAC
    ACAATCAACG GTGATATTAA TATTGACTCT GTTTTGACGA AGACCAAAAA GAAGACACTG
    AATCCAACCT GGAATGAAGA GTTCATATTT AGAGTTAAAC CGTCTGAGCA TAAGCTTGTG
    TTTCAAGTAT TTGACGAGAA TCGCCTGACA CGCGACGACT TCCTGGGCAT GGTGGAGCTG
    ACCCTGGTGA ATCTGCCTAC CGAGCAGGAA GGTCGCACCA TCGGAGAACA AAGCTACACT
    CTGCGCCCGC GCAGGTCAGT AGGCGCCAAA TCCCGCATCA AGGGCACACT GCGCATCTAC
    CACGCCTTTA TCCGGGAGAC GCGGGAGCAG AGCGAGCCAT CTAGTGGCAA TAGCGATGGC
    GAGTGGGAGC ACGTTGAGGC CACCAATGCC GGTGAGACGT CTGCCCAGCC GCACCCCTTT
    CCTACTGGTG GCCACGATGC CCTACCCGCT GGATGGGAAG AACGCCAGGA TGCCAACGGG
    CGCACGTACT ATGTGAATCA CACGGCGAGG ACGACGCAAT GGGATAGGCC TACTGTCTTA
    AACAGCCACA GTAGCCAATC CACTGACGAC CAGTTGGCCT CGGATTTCCA GAGACGTTTC
    CACATCAGCG TGGACGACAC GGAGTCAGGA CGTTCGGCGG ACTCCATCAG TCACAATAGT
    ATAGAGGACA ATAACAATGC TGCTGGCTTA GCATATACTC CAAAAACAGC AGCCACCTCA
    TCGGCACCAC CCAACACCCC AACAAACAAC AATGGAATTC TCGCACAAAT TGCCATGCAA
    TATCGCGCAG AGGAAGATCA AGATCCAACA GTGGACCATA CCAGTTTTGT TTACAATTCC
    CTGCGACATC CTGTCGCCCA TAGGCAGCCC GAAATCTCAG CGACCAGTTT GCAGAACGAC
    CTGCGGCCGG TACGGGAGGC TCCCGGTGTG CCGGATATAG CCATAACCAA CCCGTTCACG
    CGGCGGGCCG CGGGCAATAT GGCCGGCGGT GCGGGATGGC AGCAAGAACG GCGGCGACAG
    CAAATGCAAC TGCATATCCA GCAGCATCAG CAGCGGCAAC AGCAACAACA GCAGAATAGA
    ATATTGAATG AAGACACCGA TCACACAGAC AGCCACAATC CCTCTGACAT CTCAGCTCCG
    TCCACACGAC GCAATTCCGA GGAAGACAAC GCGGCTGTGC CTCCCATGGA ACAAAATACT
    GGTGGTGAAG AGGAACCCCT GCCACCGCGC TGGTCGATGC AGGTAGCCCC CAACGGAAGG
    ACATTCTTCA TTGATCATGC GTCCCGGAGG ACTACTTGGA TAGACCCGCG CAACGGACGG
    GCCAGCCCTA TGCCAAATCA AACTCGTCGC GTCGAGGACG ACCTGGGTCC TTTACCAGAG
    GGCTGGGAGG AGCGAGTACA CACCGACGGA CGCGTATTCT ATATAGATCA TAACACGCGA
    ACTACGCAGT GGGAAGATCC TCGCTTGTCT AATCCAAATA TTGCCGGTCA GGCTGTGCCC
    TATTCTCGAG ATTACAAGCA AAAATACGAG TATTTCAAGA GTCACATTAG AAAGCCTACA
    AATGTACCAA ATAAATTTGA AATACGTATT CGTCGTACGT CGATACTGGA GGATTCTTAC
    AGAATTATTA GTTCGGTAAC GAAGACCGAT TTACTAAAGA CTAAATTATG GGTAGAGTTT
    GAAGGAGAAA CTGGCTTAGA TTATGGAGGC CTCGCAAGGG AATGGTTCTA TTTATTATCA
    AAAGAAATGT TTAATCCGTA CTACGGACTA TTCGAGTACT CGGCCATGGA CAACTACACG
    CTTCAGATAA ACAATGGTAG TGGTTTGTGC AACGAGGAGC ATTTAAGTTA CTTTAAATTC
    ATTGGCCGTA TTGCGGGCAT GGCTGTGTAT CATGGAAAGC TGCTAGATGC CTTCTTCATT
    CGTCCATTCT ACAAGATGAT GCTGCAGAAG CCGATTGACC TAAAGGACAT GGAGTCTGTG
    GACACGGAGT ACTACAACTC GCTGATGTGG ATTAAGGAGA ACGATCCACG CATTCTGGAA
    CTGACTTTCT GCCTGGATGA AGACGTTTTT GGCCAGAAGA GTCAGCACGA ACTAAAGCCC
    GGCGGTGCCA ACATAGACGT GACTAACGAA AACAAGGATG AATACATTAA ATTGGTTATC
    GAATGGCGCT TTGTGGCACG TGTCAAGGAA CAGATGTCGT CCTTCCTTGA TGGTTTTGGA
    TCGATAATTC CTCTGAATCT TATTAAGATT TTCGACGAGC ACGAACTGGA GCTACTGATG
    TGTGGCATAC AGAACATCGA TGTGAAGGAT TGGCGCGAGA ATACTCTGTA CAAGGGCGAC
    TACCACATGA ATCATATTAT TATTCAGTGG TTCTGGAGGG CTGTACTTTC CTTCTCCAAC
    GAGATGCGTT CTCGTCTGCT GCAATTTGTT ACGGGCACTT CACGCGTGCC CATGAACGGT
    TTCAAGGAGC TGTACGGCTC AAATGGCCCC CAGATGTTTA CTATCGAAAA GTGGGGCACT
    CCTAATAACT TTCCAAGGGC ACATACCTGT TTCAATCGCC TGGACCTGCC ACCCTATGAA
    GGCTACCTGC AACTGAAGGA CAAACTGATC AAGGCCATCG AGGGCAGCCA AGGATTTGCT
    GGAGTTGATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa18834
    Clone ID Related Accession (Same CDS sequence) NM_001144493.3
    Accession Version NM_001144493.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2985bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product Nedd4, isoform H
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_001144493.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTCGGCAC GTTCCAGCGG CTTGGTGGCC GCGGCGGCCC TTCCCGTCCC GTCGTCTTCT 
    TCCTCGGTGG CCGGCGGCGA CGTGCCACGC CCGCCGCCCC GCCGGCGAGC GGCCTCTGTT
    GCGGGACAAC AGCAAACGCG CCAGGAATTT GGAAATGGTT ACACTCCTCG CAGATCGTTG
    GCAGCAGTGA ACGACTCTGG GGACTCGTGT CACCTGCGAA TCGTCGTACT CACCGGCCAG
    TCGCTGGCCA AAAAGGATAT ATTCGGCGCC AGCGACCCGT ATGTCAGAAT CGATTTGAAC
    ACAATCAACG GTGATATTAA TATTGACTCT GTTTTGACGA AGACCAAAAA GAAGACACTG
    AATCCAACCT GGAATGAAGA GTTCATATTT AGAGTTAAAC CGTCTGAGCA TAAGCTTGTG
    TTTCAAGTAT TTGACGAGAA TCGCCTGACA CGCGACGACT TCCTGGGCAT GGTGGAGCTG
    ACCCTGGTGA ATCTGCCTAC CGAGCAGGAA GGTCGCACCA TCGGAGAACA AAGCTACACT
    CTGCGCCCGC GCAGGTCAGT AGGCGCCAAA TCCCGCATCA AGGGCACACT GCGCATCTAC
    CACGCCTTTA TCCGGGAGAC GCGGGAGCAG AGCGAGCCAT CTAGTGGCAA TAGCGATGGC
    GAGTGGGAGC ACGTTGAGGC CACCAATGCC GGTGAGACGT CTGCCCAGCC GCACCCCTTT
    CCTACTGGTG GCCACGATGC CCTACCCGCT GGATGGGAAG AACGCCAGGA TGCCAACGGG
    CGCACGTACT ATGTGAATCA CACGGCGAGG ACGACGCAAT GGGATAGGCC TACTGTCTTA
    AACAGCCACA GTAGCCAATC CACTGACGAC CAGTTGGCCT CGGATTTCCA GAGACGTTTC
    CACATCAGCG TGGACGACAC GGAGTCAGGA CGTTCGGCGG ACTCCATCAG TCACAATAGT
    ATAGAGGACA ATAACAATGC TGCTGGCTTA GCATATACTC CAAAAACAGC AGCCACCTCA
    TCGGCACCAC CCAACACCCC AACAAACAAC AATGGAATTC TCGCACAAAT TGCCATGCAA
    TATCGCGCAG AGGAAGATCA AGATCCAACA GTGGACCATA CCAGGCAGCC CGAAATCTCA
    GCGACCAGTT TGCAGAACGA CCTGCGGCCG GTACGGGAGG CTCCCGGTGT GCCGGATATA
    GCCATAACCA ACCCGTTCAC GCGGCGGGCC GCGGGCAATA TGGCCGGCGG TGCGGGATGG
    CAGCAAGAAC GGCGGCGACA GCAAATGCAA CTGCATATCC AGCAGCATCA GCAGCGGCAA
    CAGCAACAAC AGCAGAATAG AATATTGCTT GATGTGGATC ATCGTCAGCA GGAGCCGCAA
    CATCGGGGTC AGAGACACCA GCAGCAACAT CGGCCATCGA ATGAAGACAC CGATCACACA
    GACAGCCACA ATCCCTCTGA CATCTCAGCT CCGTCCACAC GACGCAATTC CGAGGAAGAC
    AACGCGGCTG TGCCTCCCAT GGAACAAAAT ACTGGTGGTG AAGAGGAACC CCTGCCACCG
    CGCTGGTCGA TGCAGGTAGC CCCCAACGGA AGGACATTCT TCATTGATCA TGCGTCCCGG
    AGGACTACTT GGATAGACCC GCGCAACGGA CGGGCCAGCC CTATGCCAAA TCAAACTCGT
    CGCGTCGAGG ACGACCTGGG TCCTTTACCA GAGGGCTGGG AGGAGCGAGT ACACACCGAC
    GGACGCGTAT TCTATATAGA TCATAACACG CGAACTACGC AGTGGGAAGA TCCTCGCTTG
    TCTAATCCAA ATATTGCCGG TCAGGCTGTG CCCTATTCTC GAGATTACAA GCAAAAATAC
    GAGTATTTCA AGAGTCACAT TAGAAAGCCT ACAAATGTAC CAAATAAATT TGAAATACGT
    ATTCGTCGTA CGTCGATACT GGAGGATTCT TACAGAATTA TTAGTTCGGT AACGAAGACC
    GATTTACTAA AGACTAAATT ATGGGTAGAG TTTGAAGGAG AAACTGGCTT AGATTATGGA
    GGCCTCGCAA GGGAATGGTT CTATTTATTA TCAAAAGAAA TGTTTAATCC GTACTACGGA
    CTATTCGAGT ACTCGGCCAT GGACAACTAC ACGCTTCAGA TAAACAATGG TAGTGGTTTG
    TGCAACGAGG AGCATTTAAG TTACTTTAAA TTCATTGGCC GTATTGCGGG CATGGCTGTG
    TATCATGGAA AGCTGCTAGA TGCCTTCTTC ATTCGTCCAT TCTACAAGAT GATGCTGCAG
    AAGCCGATTG ACCTAAAGGA CATGGAGTCT GTGGACACGG AGTACTACAA CTCGCTGATG
    TGGATTAAGG AGAACGATCC ACGCATTCTG GAACTGACTT TCTGCCTGGA TGAAGACGTT
    TTTGGCCAGA AGAGTCAGCA CGAACTAAAG CCCGGCGGTG CCAACATAGA CGTGACTAAC
    GAAAACAAGG ATGAATACAT TAAATTGGTT ATCGAATGGC GCTTTGTGGC ACGTGTCAAG
    GAACAGATGT CGTCCTTCCT TGATGGTTTT GGATCGATAA TTCCTCTGAA TCTTATTAAG
    ATTTTCGACG AGCACGAACT GGAGCTACTG ATGTGTGGCA TACAGAACAT CGATGTGAAG
    GATTGGCGCG AGAATACTCT GTACAAGGGC GACTACCACA TGAATCATAT TATTATTCAG
    TGGTTCTGGA GGGCTGTACT TTCCTTCTCC AACGAGATGC GTTCTCGTCT GCTGCAATTT
    GTTACGGGCA CTTCACGCGT GCCCATGAAC GGTTTCAAGG AGCTGTACGG CTCAAATGGC
    CCCCAGATGT TTACTATCGA AAAGTGGGGC ACTCCTAATA ACTTTCCAAG GGCACATACC
    TGTTTCAATC GCCTGGACCT GCCACCCTAT GAAGGCTACC TGCAACTGAA GGACAAACTG
    ATCAAGGCCA TCGAGGGCAG CCAAGGATTT GCTGGAGTTG ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001137965.1
    CDS381..3365
    Translation

    Target ORF information:

    RefSeq Version NM_001144493.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster Nedd4, transcript variant H (Nedd4), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001144493.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTCGGCAC GTTCCAGCGG CTTGGTGGCC GCGGCGGCCC TTCCCGTCCC GTCGTCTTCT 
    TCCTCGGTGG CCGGCGGCGA CGTGCCACGC CCGCCGCCCC GCCGGCGAGC GGCCTCTGTT
    GCGGGACAAC AGCAAACGCG CCAGGAATTT GGAAATGGTT ACACTCCTCG CAGATCGTTG
    GCAGCAGTGA ACGACTCTGG GGACTCGTGT CACCTGCGAA TCGTCGTACT CACCGGCCAG
    TCGCTGGCCA AAAAGGATAT ATTCGGCGCC AGCGACCCGT ATGTCAGAAT CGATTTGAAC
    ACAATCAACG GTGATATTAA TATTGACTCT GTTTTGACGA AGACCAAAAA GAAGACACTG
    AATCCAACCT GGAATGAAGA GTTCATATTT AGAGTTAAAC CGTCTGAGCA TAAGCTTGTG
    TTTCAAGTAT TTGACGAGAA TCGCCTGACA CGCGACGACT TCCTGGGCAT GGTGGAGCTG
    ACCCTGGTGA ATCTGCCTAC CGAGCAGGAA GGTCGCACCA TCGGAGAACA AAGCTACACT
    CTGCGCCCGC GCAGGTCAGT AGGCGCCAAA TCCCGCATCA AGGGCACACT GCGCATCTAC
    CACGCCTTTA TCCGGGAGAC GCGGGAGCAG AGCGAGCCAT CTAGTGGCAA TAGCGATGGC
    GAGTGGGAGC ACGTTGAGGC CACCAATGCC GGTGAGACGT CTGCCCAGCC GCACCCCTTT
    CCTACTGGTG GCCACGATGC CCTACCCGCT GGATGGGAAG AACGCCAGGA TGCCAACGGG
    CGCACGTACT ATGTGAATCA CACGGCGAGG ACGACGCAAT GGGATAGGCC TACTGTCTTA
    AACAGCCACA GTAGCCAATC CACTGACGAC CAGTTGGCCT CGGATTTCCA GAGACGTTTC
    CACATCAGCG TGGACGACAC GGAGTCAGGA CGTTCGGCGG ACTCCATCAG TCACAATAGT
    ATAGAGGACA ATAACAATGC TGCTGGCTTA GCATATACTC CAAAAACAGC AGCCACCTCA
    TCGGCACCAC CCAACACCCC AACAAACAAC AATGGAATTC TCGCACAAAT TGCCATGCAA
    TATCGCGCAG AGGAAGATCA AGATCCAACA GTGGACCATA CCAGGCAGCC CGAAATCTCA
    GCGACCAGTT TGCAGAACGA CCTGCGGCCG GTACGGGAGG CTCCCGGTGT GCCGGATATA
    GCCATAACCA ACCCGTTCAC GCGGCGGGCC GCGGGCAATA TGGCCGGCGG TGCGGGATGG
    CAGCAAGAAC GGCGGCGACA GCAAATGCAA CTGCATATCC AGCAGCATCA GCAGCGGCAA
    CAGCAACAAC AGCAGAATAG AATATTGCTT GATGTGGATC ATCGTCAGCA GGAGCCGCAA
    CATCGGGGTC AGAGACACCA GCAGCAACAT CGGCCATCGA ATGAAGACAC CGATCACACA
    GACAGCCACA ATCCCTCTGA CATCTCAGCT CCGTCCACAC GACGCAATTC CGAGGAAGAC
    AACGCGGCTG TGCCTCCCAT GGAACAAAAT ACTGGTGGTG AAGAGGAACC CCTGCCACCG
    CGCTGGTCGA TGCAGGTAGC CCCCAACGGA AGGACATTCT TCATTGATCA TGCGTCCCGG
    AGGACTACTT GGATAGACCC GCGCAACGGA CGGGCCAGCC CTATGCCAAA TCAAACTCGT
    CGCGTCGAGG ACGACCTGGG TCCTTTACCA GAGGGCTGGG AGGAGCGAGT ACACACCGAC
    GGACGCGTAT TCTATATAGA TCATAACACG CGAACTACGC AGTGGGAAGA TCCTCGCTTG
    TCTAATCCAA ATATTGCCGG TCAGGCTGTG CCCTATTCTC GAGATTACAA GCAAAAATAC
    GAGTATTTCA AGAGTCACAT TAGAAAGCCT ACAAATGTAC CAAATAAATT TGAAATACGT
    ATTCGTCGTA CGTCGATACT GGAGGATTCT TACAGAATTA TTAGTTCGGT AACGAAGACC
    GATTTACTAA AGACTAAATT ATGGGTAGAG TTTGAAGGAG AAACTGGCTT AGATTATGGA
    GGCCTCGCAA GGGAATGGTT CTATTTATTA TCAAAAGAAA TGTTTAATCC GTACTACGGA
    CTATTCGAGT ACTCGGCCAT GGACAACTAC ACGCTTCAGA TAAACAATGG TAGTGGTTTG
    TGCAACGAGG AGCATTTAAG TTACTTTAAA TTCATTGGCC GTATTGCGGG CATGGCTGTG
    TATCATGGAA AGCTGCTAGA TGCCTTCTTC ATTCGTCCAT TCTACAAGAT GATGCTGCAG
    AAGCCGATTG ACCTAAAGGA CATGGAGTCT GTGGACACGG AGTACTACAA CTCGCTGATG
    TGGATTAAGG AGAACGATCC ACGCATTCTG GAACTGACTT TCTGCCTGGA TGAAGACGTT
    TTTGGCCAGA AGAGTCAGCA CGAACTAAAG CCCGGCGGTG CCAACATAGA CGTGACTAAC
    GAAAACAAGG ATGAATACAT TAAATTGGTT ATCGAATGGC GCTTTGTGGC ACGTGTCAAG
    GAACAGATGT CGTCCTTCCT TGATGGTTTT GGATCGATAA TTCCTCTGAA TCTTATTAAG
    ATTTTCGACG AGCACGAACT GGAGCTACTG ATGTGTGGCA TACAGAACAT CGATGTGAAG
    GATTGGCGCG AGAATACTCT GTACAAGGGC GACTACCACA TGAATCATAT TATTATTCAG
    TGGTTCTGGA GGGCTGTACT TTCCTTCTCC AACGAGATGC GTTCTCGTCT GCTGCAATTT
    GTTACGGGCA CTTCACGCGT GCCCATGAAC GGTTTCAAGG AGCTGTACGG CTCAAATGGC
    CCCCAGATGT TTACTATCGA AAAGTGGGGC ACTCCTAATA ACTTTCCAAG GGCACATACC
    TGTTTCAATC GCCTGGACCT GCCACCCTAT GAAGGCTACC TGCAACTGAA GGACAAACTG
    ATCAAGGCCA TCGAGGGCAG CCAAGGATTT GCTGGAGTTG ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa18835
    Clone ID Related Accession (Same CDS sequence) NM_001275062.1
    Accession Version NM_001275062.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3000bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product Nedd4, isoform M
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTCGGCAC GTTCCAGCGG CTTGGTGGCC GCGGCGGCCC TTCCCGTCCC GTCGTCTTCT 
    TCCTCGGTGG CCGGCGGCGA CGTGCCACGC CCGCCGCCCC GCCGGCGAGC GGCCTCTGTT
    GCGGGACAAC AGCAAACGCG CCAGGAATTT GGAAATGGTT ACACTCCTCG CAGATCGTTG
    GCAGCAGTGA ACGACTCTGG GGACTCGTGT CACCTGCGAA TCGTCGTACT CACCGGCCAG
    TCGCTGGCCA AAAAGGATAT ATTCGGCGCC AGCGACCCGT ATGTCAGAAT CGATTTGAAC
    ACAATCAACG GTGATATTAA TATTGACTCT GTTTTGACGA AGACCAAAAA GAAGACACTG
    AATCCAACCT GGAATGAAGA GTTCATATTT AGAGTTAAAC CGTCTGAGCA TAAGCTTGTG
    TTTCAAGTAT TTGACGAGAA TCGCCTGACA CGCGACGACT TCCTGGGCAT GGTGGAGCTG
    ACCCTGGTGA ATCTGCCTAC CGAGCAGGAA GGTCGCACCA TCGGAGAACA AAGCTACACT
    CTGCGCCCGC GCAGCGCCAA ATCCCGCATC AAGGGCACAC TGCGCATCTA CCACGCCTTT
    ATCCGGGAGA CGCGGGAGCA GAGCGAGCCA TCTAGTGGCA ATAGCGATGG CGAGTGGGAG
    CACGTTGAGG CCACCAATGC CGGTGAGACG TCTGCCCAGC CGCACCCCTT TCCTACTGGT
    GGCCACGATG CCCTACCCGC TGGATGGGAA GAACGCCAGG ATGCCAACGG GCGCACGTAC
    TATGTGAATC ACACGGCGAG GACGACGCAA TGGGATAGGC CTACTGTCTT AAACAGCCAC
    AGTAGCCAAT CCACTGACGA CCAGTTGGCC TCGGATTTCC AGAGACGTTT CCACATCAGC
    GTGGACGACA CGGAGTCAGG ACGTTCGGCG GACTCCATCA GTCACAATAG TATAGAGGAC
    AATAACAATG CTGCTGGCTT AGCATATACT CCAAAAACAG CAGCCACCTC ATCGGCACCA
    CCCAACACCC CAACAAACAA CAATGGAATT CTCGCACAAA TTGCCATGCA ATATCGCGCA
    GAGGAAGATC AAGATCCAAC AGTGGACCAT ACCAGTTTTG TTTACAATTC CCTGCGACAT
    CCTGTCGCCC ATAGGCAGCC CGAAATCTCA GCGACCAGTT TGCAGAACGA CCTGCGGCCG
    GTACGGGAGG CTCCCGGTGT GCCGGATATA GCCATAACCA ACCCGTTCAC GCGGCGGGCC
    GCGGGCAATA TGGCCGGCGG TGCGGGATGG CAGCAAGAAC GGCGGCGACA GCAAATGCAA
    CTGCATATCC AGCAGCATCA GCAGCGGCAA CAGCAACAAC AGCAGAATAG AATATTGATA
    AATACACATA TATTTGTTTG TTTTAGGACA TGGGGGATTT CAGTGGGCGG AGCGAATGAA
    GACACCGATC ACACAGACAG CCACAATCCC TCTGACATCT CAGCTCCGTC CACACGACGC
    AATTCCGAGG AAGACAACGC GGCTGTGCCT CCCATGGAAC AAAATACTGG TGGTGAAGAG
    GAACCCCTGC CACCGCGCTG GTCGATGCAG GTAGCCCCCA ACGGAAGGAC ATTCTTCATT
    GATCATGCGT CCCGGAGGAC TACTTGGATA GACCCGCGCA ACGGACGGGC CAGCCCTATG
    CCAAATCAAA CTCGTCGCGT CGAGGACGAC CTGGGTCCTT TACCAGAGGG CTGGGAGGAG
    CGAGTACACA CCGACGGACG CGTATTCTAT ATAGATCATA ACACGCGAAC TACGCAGTGG
    GAAGATCCTC GCTTGTCTAA TCCAAATATT GCCGGTCAGG CTGTGCCCTA TTCTCGAGAT
    TACAAGCAAA AATACGAGTA TTTCAAGAGT CACATTAGAA AGCCTACAAA TGTACCAAAT
    AAATTTGAAA TACGTATTCG TCGTACGTCG ATACTGGAGG ATTCTTACAG AATTATTAGT
    TCGGTAACGA AGACCGATTT ACTAAAGACT AAATTATGGG TAGAGTTTGA AGGAGAAACT
    GGCTTAGATT ATGGAGGCCT CGCAAGGGAA TGGTTCTATT TATTATCAAA AGAAATGTTT
    AATCCGTACT ACGGACTATT CGAGTACTCG GCCATGGACA ACTACACGCT TCAGATAAAC
    AATGGTAGTG GTTTGTGCAA CGAGGAGCAT TTAAGTTACT TTAAATTCAT TGGCCGTATT
    GCGGGCATGG CTGTGTATCA TGGAAAGCTG CTAGATGCCT TCTTCATTCG TCCATTCTAC
    AAGATGATGC TGCAGAAGCC GATTGACCTA AAGGACATGG AGTCTGTGGA CACGGAGTAC
    TACAACTCGC TGATGTGGAT TAAGGAGAAC GATCCACGCA TTCTGGAACT GACTTTCTGC
    CTGGATGAAG ACGTTTTTGG CCAGAAGAGT CAGCACGAAC TAAAGCCCGG CGGTGCCAAC
    ATAGACGTGA CTAACGAAAA CAAGGATGAA TACATTAAAT TGGTTATCGA ATGGCGCTTT
    GTGGCACGTG TCAAGGAACA GATGTCGTCC TTCCTTGATG GTTTTGGATC GATAATTCCT
    CTGAATCTTA TTAAGATTTT CGACGAGCAC GAACTGGAGC TACTGATGTG TGGCATACAG
    AACATCGATG TGAAGGATTG GCGCGAGAAT ACTCTGTACA AGGGCGACTA CCACATGAAT
    CATATTATTA TTCAGTGGTT CTGGAGGGCT GTACTTTCCT TCTCCAACGA GATGCGTTCT
    CGTCTGCTGC AATTTGTTAC GGGCACTTCA CGCGTGCCCA TGAACGGTTT CAAGGAGCTG
    TACGGCTCAA ATGGCCCCCA GATGTTTACT ATCGAAAAGT GGGGCACTCC TAATAACTTT
    CCAAGGGCAC ATACCTGTTT CAATCGCCTG GACCTGCCAC CCTATGAAGG CTACCTGCAA
    CTGAAGGACA AACTGATCAA GGCCATCGAG GGCAGCCAAG GATTTGCTGG AGTTGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001261991.1
    CDS381..3380
    Translation

    Target ORF information:

    RefSeq Version NM_001275062.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster Nedd4, transcript variant M (Nedd4), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001275062.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTCGGCAC GTTCCAGCGG CTTGGTGGCC GCGGCGGCCC TTCCCGTCCC GTCGTCTTCT 
    TCCTCGGTGG CCGGCGGCGA CGTGCCACGC CCGCCGCCCC GCCGGCGAGC GGCCTCTGTT
    GCGGGACAAC AGCAAACGCG CCAGGAATTT GGAAATGGTT ACACTCCTCG CAGATCGTTG
    GCAGCAGTGA ACGACTCTGG GGACTCGTGT CACCTGCGAA TCGTCGTACT CACCGGCCAG
    TCGCTGGCCA AAAAGGATAT ATTCGGCGCC AGCGACCCGT ATGTCAGAAT CGATTTGAAC
    ACAATCAACG GTGATATTAA TATTGACTCT GTTTTGACGA AGACCAAAAA GAAGACACTG
    AATCCAACCT GGAATGAAGA GTTCATATTT AGAGTTAAAC CGTCTGAGCA TAAGCTTGTG
    TTTCAAGTAT TTGACGAGAA TCGCCTGACA CGCGACGACT TCCTGGGCAT GGTGGAGCTG
    ACCCTGGTGA ATCTGCCTAC CGAGCAGGAA GGTCGCACCA TCGGAGAACA AAGCTACACT
    CTGCGCCCGC GCAGCGCCAA ATCCCGCATC AAGGGCACAC TGCGCATCTA CCACGCCTTT
    ATCCGGGAGA CGCGGGAGCA GAGCGAGCCA TCTAGTGGCA ATAGCGATGG CGAGTGGGAG
    CACGTTGAGG CCACCAATGC CGGTGAGACG TCTGCCCAGC CGCACCCCTT TCCTACTGGT
    GGCCACGATG CCCTACCCGC TGGATGGGAA GAACGCCAGG ATGCCAACGG GCGCACGTAC
    TATGTGAATC ACACGGCGAG GACGACGCAA TGGGATAGGC CTACTGTCTT AAACAGCCAC
    AGTAGCCAAT CCACTGACGA CCAGTTGGCC TCGGATTTCC AGAGACGTTT CCACATCAGC
    GTGGACGACA CGGAGTCAGG ACGTTCGGCG GACTCCATCA GTCACAATAG TATAGAGGAC
    AATAACAATG CTGCTGGCTT AGCATATACT CCAAAAACAG CAGCCACCTC ATCGGCACCA
    CCCAACACCC CAACAAACAA CAATGGAATT CTCGCACAAA TTGCCATGCA ATATCGCGCA
    GAGGAAGATC AAGATCCAAC AGTGGACCAT ACCAGTTTTG TTTACAATTC CCTGCGACAT
    CCTGTCGCCC ATAGGCAGCC CGAAATCTCA GCGACCAGTT TGCAGAACGA CCTGCGGCCG
    GTACGGGAGG CTCCCGGTGT GCCGGATATA GCCATAACCA ACCCGTTCAC GCGGCGGGCC
    GCGGGCAATA TGGCCGGCGG TGCGGGATGG CAGCAAGAAC GGCGGCGACA GCAAATGCAA
    CTGCATATCC AGCAGCATCA GCAGCGGCAA CAGCAACAAC AGCAGAATAG AATATTGATA
    AATACACATA TATTTGTTTG TTTTAGGACA TGGGGGATTT CAGTGGGCGG AGCGAATGAA
    GACACCGATC ACACAGACAG CCACAATCCC TCTGACATCT CAGCTCCGTC CACACGACGC
    AATTCCGAGG AAGACAACGC GGCTGTGCCT CCCATGGAAC AAAATACTGG TGGTGAAGAG
    GAACCCCTGC CACCGCGCTG GTCGATGCAG GTAGCCCCCA ACGGAAGGAC ATTCTTCATT
    GATCATGCGT CCCGGAGGAC TACTTGGATA GACCCGCGCA ACGGACGGGC CAGCCCTATG
    CCAAATCAAA CTCGTCGCGT CGAGGACGAC CTGGGTCCTT TACCAGAGGG CTGGGAGGAG
    CGAGTACACA CCGACGGACG CGTATTCTAT ATAGATCATA ACACGCGAAC TACGCAGTGG
    GAAGATCCTC GCTTGTCTAA TCCAAATATT GCCGGTCAGG CTGTGCCCTA TTCTCGAGAT
    TACAAGCAAA AATACGAGTA TTTCAAGAGT CACATTAGAA AGCCTACAAA TGTACCAAAT
    AAATTTGAAA TACGTATTCG TCGTACGTCG ATACTGGAGG ATTCTTACAG AATTATTAGT
    TCGGTAACGA AGACCGATTT ACTAAAGACT AAATTATGGG TAGAGTTTGA AGGAGAAACT
    GGCTTAGATT ATGGAGGCCT CGCAAGGGAA TGGTTCTATT TATTATCAAA AGAAATGTTT
    AATCCGTACT ACGGACTATT CGAGTACTCG GCCATGGACA ACTACACGCT TCAGATAAAC
    AATGGTAGTG GTTTGTGCAA CGAGGAGCAT TTAAGTTACT TTAAATTCAT TGGCCGTATT
    GCGGGCATGG CTGTGTATCA TGGAAAGCTG CTAGATGCCT TCTTCATTCG TCCATTCTAC
    AAGATGATGC TGCAGAAGCC GATTGACCTA AAGGACATGG AGTCTGTGGA CACGGAGTAC
    TACAACTCGC TGATGTGGAT TAAGGAGAAC GATCCACGCA TTCTGGAACT GACTTTCTGC
    CTGGATGAAG ACGTTTTTGG CCAGAAGAGT CAGCACGAAC TAAAGCCCGG CGGTGCCAAC
    ATAGACGTGA CTAACGAAAA CAAGGATGAA TACATTAAAT TGGTTATCGA ATGGCGCTTT
    GTGGCACGTG TCAAGGAACA GATGTCGTCC TTCCTTGATG GTTTTGGATC GATAATTCCT
    CTGAATCTTA TTAAGATTTT CGACGAGCAC GAACTGGAGC TACTGATGTG TGGCATACAG
    AACATCGATG TGAAGGATTG GCGCGAGAAT ACTCTGTACA AGGGCGACTA CCACATGAAT
    CATATTATTA TTCAGTGGTT CTGGAGGGCT GTACTTTCCT TCTCCAACGA GATGCGTTCT
    CGTCTGCTGC AATTTGTTAC GGGCACTTCA CGCGTGCCCA TGAACGGTTT CAAGGAGCTG
    TACGGCTCAA ATGGCCCCCA GATGTTTACT ATCGAAAAGT GGGGCACTCC TAATAACTTT
    CCAAGGGCAC ATACCTGTTT CAATCGCCTG GACCTGCCAC CCTATGAAGG CTACCTGCAA
    CTGAAGGACA AACTGATCAA GGCCATCGAG GGCAGCCAAG GATTTGCTGG AGTTGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa21013
    Clone ID Related Accession (Same CDS sequence) NM_140736.3
    Accession Version NM_140736.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3024bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product Nedd4, isoform J
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_140736.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGTCGGCAC GTTCCAGCGG CTTGGTGGCC GCGGCGGCCC TTCCCGTCCC GTCGTCTTCT 
    TCCTCGGTGG CCGGCGGCGA CGTGCCACGC CCGCCGCCCC GCCGGCGAGC GGCCTCTGTT
    GCGGGACAAC AGCAAACGCG CCAGGAATTT GGAAATGGTT ACACTCCTCG CAGATCGTTG
    GCAGCAGTGA ACGACTCTGG GGACTCGTGT CACCTGCGAA TCGTCGTACT CACCGGCCAG
    TCGCTGGCCA AAAAGGATAT ATTCGGCGCC AGCGACCCGT ATGTCAGAAT CGATTTGAAC
    ACAATCAACG GTGATATTAA TATTGACTCT GTTTTGACGA AGACCAAAAA GAAGACACTG
    AATCCAACCT GGAATGAAGA GTTCATATTT AGAGTTAAAC CGTCTGAGCA TAAGCTTGTG
    TTTCAAGTAT TTGACGAGAA TCGCCTGACA CGCGACGACT TCCTGGGCAT GGTGGAGCTG
    ACCCTGGTGA ATCTGCCTAC CGAGCAGGAA GGTCGCACCA TCGGAGAACA AAGCTACACT
    CTGCGCCCGC GCAGGTCAGT AGGCGCCAAA TCCCGCATCA AGGGCACACT GCGCATCTAC
    CACGCCTTTA TCCGGGAGAC GCGGGAGCAG AGCGAGCCAT CTAGTGGCAA TAGCGATGGC
    GAGTGGGAGC ACGTTGAGGC CACCAATGCC GGTGAGACGT CTGCCCAGCC GCACCCCTTT
    CCTACTGGTG GCCACGATGC CCTACCCGCT GGATGGGAAG AACGCCAGGA TGCCAACGGG
    CGCACGTACT ATGTGAATCA CACGGCGAGG ACGACGCAAT GGGATAGGCC TACTGTCTTA
    AACAGCCACA GTAGCCAATC CACTGACGAC CAGTTGGCCT CGGATTTCCA GAGACGTTTC
    CACATCAGCG TGGACGACAC GGAGTCAGGA CGTTCGGCGG ACTCCATCAG TCACAATAGT
    ATAGAGGACA ATAACAATGC TGCTGGCTTA GCATATACTC CAAAAACAGC AGCCACCTCA
    TCGGCACCAC CCAACACCCC AACAAACAAC AATGGAATTC TCGCACAAAT TGCCATGCAA
    TATCGCGCAG AGGAAGATCA AGATCCAACA GTGGACCATA CCAGTTTTGT TTACAATTCC
    CTGCGACATC CTGTCGCCCA TAGGCAGCCC GAAATCTCAG CGACCAGTTT GCAGAACGAC
    CTGCGGCCGG TACGGGAGGC TCCCGGTGTG CCGGATATAG CCATAACCAA CCCGTTCACG
    CGGCGGGCCG CGGGCAATAT GGCCGGCGGT GCGGGATGGC AGCAAGAACG GCGGCGACAG
    CAAATGCAAC TGCATATCCA GCAGCATCAG CAGCGGCAAC AGCAACAACA GCAGAATAGA
    ATATTGCTTG ATGTGGATCA TCGTCAGCAG GAGCCGCAAC ATCGGGGTCA GAGACACCAG
    CAGCAACATC GGCCATCGAA TGAAGACACC GATCACACAG ACAGCCACAA TCCCTCTGAC
    ATCTCAGCTC CGTCCACACG ACGCAATTCC GAGGAAGACA ACGCGGCTGT GCCTCCCATG
    GAACAAAATA CTGGTGGTGA AGAGGAACCC CTGCCACCGC GCTGGTCGAT GCAGGTAGCC
    CCCAACGGAA GGACATTCTT CATTGATCAT GCGTCCCGGA GGACTACTTG GATAGACCCG
    CGCAACGGAC GGGCCAGCCC TATGCCAAAT CAAACTCGTC GCGTCGAGGA CGACCTGGGT
    CCTTTACCAG AGGGCTGGGA GGAGCGAGTA CACACCGACG GACGCGTATT CTATATAGAT
    CATAACACGC GAACTACGCA GTGGGAAGAT CCTCGCTTGT CTAATCCAAA TATTGCCGGT
    CAGGCTGTGC CCTATTCTCG AGATTACAAG CAAAAATACG AGTATTTCAA GAGTCACATT
    AGAAAGCCTA CAAATGTACC AAATAAATTT GAAATACGTA TTCGTCGTAC GTCGATACTG
    GAGGATTCTT ACAGAATTAT TAGTTCGGTA ACGAAGACCG ATTTACTAAA GACTAAATTA
    TGGGTAGAGT TTGAAGGAGA AACTGGCTTA GATTATGGAG GCCTCGCAAG GGAATGGTTC
    TATTTATTAT CAAAAGAAAT GTTTAATCCG TACTACGGAC TATTCGAGTA CTCGGCCATG
    GACAACTACA CGCTTCAGAT AAACAATGGT AGTGGTTTGT GCAACGAGGA GCATTTAAGT
    TACTTTAAAT TCATTGGCCG TATTGCGGGC ATGGCTGTGT ATCATGGAAA GCTGCTAGAT
    GCCTTCTTCA TTCGTCCATT CTACAAGATG ATGCTGCAGA AGCCGATTGA CCTAAAGGAC
    ATGGAGTCTG TGGACACGGA GTACTACAAC TCGCTGATGT GGATTAAGGA GAACGATCCA
    CGCATTCTGG AACTGACTTT CTGCCTGGAT GAAGACGTTT TTGGCCAGAA GAGTCAGCAC
    GAACTAAAGC CCGGCGGTGC CAACATAGAC GTGACTAACG AAAACAAGGA TGAATACATT
    AAATTGGTTA TCGAATGGCG CTTTGTGGCA CGTGTCAAGG AACAGATGTC GTCCTTCCTT
    GATGGTTTTG GATCGATAAT TCCTCTGAAT CTTATTAAGA TTTTCGACGA GCACGAACTG
    GAGCTACTGA TGTGTGGCAT ACAGAACATC GATGTGAAGG ATTGGCGCGA GAATACTCTG
    TACAAGGGCG ACTACCACAT GAATCATATT ATTATTCAGT GGTTCTGGAG GGCTGTACTT
    TCCTTCTCCA ACGAGATGCG TTCTCGTCTG CTGCAATTTG TTACGGGCAC TTCACGCGTG
    CCCATGAACG GTTTCAAGGA GCTGTACGGC TCAAATGGCC CCCAGATGTT TACTATCGAA
    AAGTGGGGCA CTCCTAATAA CTTTCCAAGG GCACATACCT GTTTCAATCG CCTGGACCTG
    CCACCCTATG AAGGCTACCT GCAACTGAAG GACAAACTGA TCAAGGCCAT CGAGGGCAGC
    CAAGGATTTG CTGGAGTTGA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_648993.1
    CDS381..3404
    Translation

    Target ORF information:

    RefSeq Version NM_140736.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster Nedd4, transcript variant J (Nedd4), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_140736.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGTCGGCAC GTTCCAGCGG CTTGGTGGCC GCGGCGGCCC TTCCCGTCCC GTCGTCTTCT 
    TCCTCGGTGG CCGGCGGCGA CGTGCCACGC CCGCCGCCCC GCCGGCGAGC GGCCTCTGTT
    GCGGGACAAC AGCAAACGCG CCAGGAATTT GGAAATGGTT ACACTCCTCG CAGATCGTTG
    GCAGCAGTGA ACGACTCTGG GGACTCGTGT CACCTGCGAA TCGTCGTACT CACCGGCCAG
    TCGCTGGCCA AAAAGGATAT ATTCGGCGCC AGCGACCCGT ATGTCAGAAT CGATTTGAAC
    ACAATCAACG GTGATATTAA TATTGACTCT GTTTTGACGA AGACCAAAAA GAAGACACTG
    AATCCAACCT GGAATGAAGA GTTCATATTT AGAGTTAAAC CGTCTGAGCA TAAGCTTGTG
    TTTCAAGTAT TTGACGAGAA TCGCCTGACA CGCGACGACT TCCTGGGCAT GGTGGAGCTG
    ACCCTGGTGA ATCTGCCTAC CGAGCAGGAA GGTCGCACCA TCGGAGAACA AAGCTACACT
    CTGCGCCCGC GCAGGTCAGT AGGCGCCAAA TCCCGCATCA AGGGCACACT GCGCATCTAC
    CACGCCTTTA TCCGGGAGAC GCGGGAGCAG AGCGAGCCAT CTAGTGGCAA TAGCGATGGC
    GAGTGGGAGC ACGTTGAGGC CACCAATGCC GGTGAGACGT CTGCCCAGCC GCACCCCTTT
    CCTACTGGTG GCCACGATGC CCTACCCGCT GGATGGGAAG AACGCCAGGA TGCCAACGGG
    CGCACGTACT ATGTGAATCA CACGGCGAGG ACGACGCAAT GGGATAGGCC TACTGTCTTA
    AACAGCCACA GTAGCCAATC CACTGACGAC CAGTTGGCCT CGGATTTCCA GAGACGTTTC
    CACATCAGCG TGGACGACAC GGAGTCAGGA CGTTCGGCGG ACTCCATCAG TCACAATAGT
    ATAGAGGACA ATAACAATGC TGCTGGCTTA GCATATACTC CAAAAACAGC AGCCACCTCA
    TCGGCACCAC CCAACACCCC AACAAACAAC AATGGAATTC TCGCACAAAT TGCCATGCAA
    TATCGCGCAG AGGAAGATCA AGATCCAACA GTGGACCATA CCAGTTTTGT TTACAATTCC
    CTGCGACATC CTGTCGCCCA TAGGCAGCCC GAAATCTCAG CGACCAGTTT GCAGAACGAC
    CTGCGGCCGG TACGGGAGGC TCCCGGTGTG CCGGATATAG CCATAACCAA CCCGTTCACG
    CGGCGGGCCG CGGGCAATAT GGCCGGCGGT GCGGGATGGC AGCAAGAACG GCGGCGACAG
    CAAATGCAAC TGCATATCCA GCAGCATCAG CAGCGGCAAC AGCAACAACA GCAGAATAGA
    ATATTGCTTG ATGTGGATCA TCGTCAGCAG GAGCCGCAAC ATCGGGGTCA GAGACACCAG
    CAGCAACATC GGCCATCGAA TGAAGACACC GATCACACAG ACAGCCACAA TCCCTCTGAC
    ATCTCAGCTC CGTCCACACG ACGCAATTCC GAGGAAGACA ACGCGGCTGT GCCTCCCATG
    GAACAAAATA CTGGTGGTGA AGAGGAACCC CTGCCACCGC GCTGGTCGAT GCAGGTAGCC
    CCCAACGGAA GGACATTCTT CATTGATCAT GCGTCCCGGA GGACTACTTG GATAGACCCG
    CGCAACGGAC GGGCCAGCCC TATGCCAAAT CAAACTCGTC GCGTCGAGGA CGACCTGGGT
    CCTTTACCAG AGGGCTGGGA GGAGCGAGTA CACACCGACG GACGCGTATT CTATATAGAT
    CATAACACGC GAACTACGCA GTGGGAAGAT CCTCGCTTGT CTAATCCAAA TATTGCCGGT
    CAGGCTGTGC CCTATTCTCG AGATTACAAG CAAAAATACG AGTATTTCAA GAGTCACATT
    AGAAAGCCTA CAAATGTACC AAATAAATTT GAAATACGTA TTCGTCGTAC GTCGATACTG
    GAGGATTCTT ACAGAATTAT TAGTTCGGTA ACGAAGACCG ATTTACTAAA GACTAAATTA
    TGGGTAGAGT TTGAAGGAGA AACTGGCTTA GATTATGGAG GCCTCGCAAG GGAATGGTTC
    TATTTATTAT CAAAAGAAAT GTTTAATCCG TACTACGGAC TATTCGAGTA CTCGGCCATG
    GACAACTACA CGCTTCAGAT AAACAATGGT AGTGGTTTGT GCAACGAGGA GCATTTAAGT
    TACTTTAAAT TCATTGGCCG TATTGCGGGC ATGGCTGTGT ATCATGGAAA GCTGCTAGAT
    GCCTTCTTCA TTCGTCCATT CTACAAGATG ATGCTGCAGA AGCCGATTGA CCTAAAGGAC
    ATGGAGTCTG TGGACACGGA GTACTACAAC TCGCTGATGT GGATTAAGGA GAACGATCCA
    CGCATTCTGG AACTGACTTT CTGCCTGGAT GAAGACGTTT TTGGCCAGAA GAGTCAGCAC
    GAACTAAAGC CCGGCGGTGC CAACATAGAC GTGACTAACG AAAACAAGGA TGAATACATT
    AAATTGGTTA TCGAATGGCG CTTTGTGGCA CGTGTCAAGG AACAGATGTC GTCCTTCCTT
    GATGGTTTTG GATCGATAAT TCCTCTGAAT CTTATTAAGA TTTTCGACGA GCACGAACTG
    GAGCTACTGA TGTGTGGCAT ACAGAACATC GATGTGAAGG ATTGGCGCGA GAATACTCTG
    TACAAGGGCG ACTACCACAT GAATCATATT ATTATTCAGT GGTTCTGGAG GGCTGTACTT
    TCCTTCTCCA ACGAGATGCG TTCTCGTCTG CTGCAATTTG TTACGGGCAC TTCACGCGTG
    CCCATGAACG GTTTCAAGGA GCTGTACGGC TCAAATGGCC CCCAGATGTT TACTATCGAA
    AAGTGGGGCA CTCCTAATAA CTTTCCAAGG GCACATACCT GTTTCAATCG CCTGGACCTG
    CCACCCTATG AAGGCTACCT GCAACTGAAG GACAAACTGA TCAAGGCCAT CGAGGGCAGC
    CAAGGATTTG CTGGAGTTGA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa21014
    Clone ID Related Accession (Same CDS sequence) NM_168738.4
    Accession Version NM_168738.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2796bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product Nedd4, isoform G
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_168738.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGTCGGCAC GTTCCAGCGG CTTGGTGGCC GCGGCGGCCC TTCCCGTCCC GTCGTCTTCT 
    TCCTCGGTGG CCGGCGGCGA CGTGCCACGC CCGCCGCCCC GCCGGCGAGC GGCCTCTGTT
    GCGGGACAAC AGCAAACGCG CCAGGAATTT GGAAATGGTT ACACTCCTCG CAGATCGTTG
    GCAGCAGTGA ACGACTCTGG GGACTCGTGT CACCTGCGAA TCGTCGTACT CACCGGCCAG
    TCGCTGGCCA AAAAGGATAT ATTCGGCGCC AGCGACCCGT ATGTCAGAAT CGATTTGAAC
    ACAATCAACG GTGATATTAA TATTGACTCT GTTTTGACGA AGACCAAAAA GAAGACACTG
    AATCCAACCT GGAATGAAGA GTTCATATTT AGAGTTAAAC CGTCTGAGCA TAAGCTTGTG
    TTTCAAGTAT TTGACGAGAA TCGCCTGACA CGCGACGACT TCCTGGGCAT GGTGGAGCTG
    ACCCTGGTGA ATCTGCCTAC CGAGCAGGAA GGTCGCACCA TCGGAGAACA AAGCTACACT
    CTGCGCCCGC GCAGGTCAGT AGGCGCCAAA TCCCGCATCA AGGGCACACT GCGCATCTAC
    CACGCCTTTA TCCGGGAGAC GCGGGAGCAG AGCGAGCCAT CTAGTGGCAA TAGCGATGGC
    GAGTGGGAGC ACGTTGAGGC CACCAATGCC GGTGAGACGT CTGCCCAGCC GCACCCCTTT
    CCTACTGGTG GCCACGATGC CCTACCCGCT GGATGGGAAG AACGCCAGGA TGCCAACGGG
    CGCACGTACT ATGTGAATCA CACGGCGAGG ACGACGCAAT GGGATAGGCC TACTGTCTTA
    AACAGCCACA GTAGCCAATC CACTGACGAC CAGTTGGCCT CGGATTTCCA GAGACGTTTC
    CACATCAGCG TGGACGACAC GGAGTCAGGA CGTTCGGCGA GGCCAAGGAA CGCTGCTCAC
    ATGGAGACTG TGGCCAGCGA TGAGAGCAGC GAGGATGAGG ATGTGGCTGA GAATGGCGAG
    GAGATGGTGG ATCTTCAGAA TAATCAGGCC AATTGTCTGC ACTGCGGCGA GCTGCATGAC
    AATGACGAGG CAGAGGAGTA TGTTGATGAG GAGGAGTATA ATGCAGAGCT GCAGCCTCCA
    ACACCTGACC CAGATCAGCC GTCAGCCACA TCCCAAAGCG CGCAAACATG TGATATACTC
    GATAGGAACT TGTACTTGAA ACGCATATTC AATGAAGACA CCGATCACAC AGACAGCCAC
    AATCCCTCTG ACATCTCAGC TCCGTCCACA CGACGCAATT CCGAGGAAGA CAACGCGGCT
    GTGCCTCCCA TGGAACAAAA TACTGGTGGT GAAGAGGAAC CCCTGCCACC GCGCTGGTCG
    ATGCAGGTAG CCCCCAACGG AAGGACATTC TTCATTGATC ATGCGTCCCG GAGGACTACT
    TGGATAGACC CGCGCAACGG ACGGGCCAGC CCTATGCCAA ATCAAACTCG TCGCGTCGAG
    GACGACCTGG GTCCTTTACC AGAGGGCTGG GAGGAGCGAG TACACACCGA CGGACGCGTA
    TTCTATATAG ATCATAACAC GCGAACTACG CAGTGGGAAG ATCCTCGCTT GTCTAATCCA
    AATATTGCCG GTCAGGCTGT GCCCTATTCT CGAGATTACA AGCAAAAATA CGAGTATTTC
    AAGAGTCACA TTAGAAAGCC TACAAATGTA CCAAATAAAT TTGAAATACG TATTCGTCGT
    ACGTCGATAC TGGAGGATTC TTACAGAATT ATTAGTTCGG TAACGAAGAC CGATTTACTA
    AAGACTAAAT TATGGGTAGA GTTTGAAGGA GAAACTGGCT TAGATTATGG AGGCCTCGCA
    AGGGAATGGT TCTATTTATT ATCAAAAGAA ATGTTTAATC CGTACTACGG ACTATTCGAG
    TACTCGGCCA TGGACAACTA CACGCTTCAG ATAAACAATG GTAGTGGTTT GTGCAACGAG
    GAGCATTTAA GTTACTTTAA ATTCATTGGC CGTATTGCGG GCATGGCTGT GTATCATGGA
    AAGCTGCTAG ATGCCTTCTT CATTCGTCCA TTCTACAAGA TGATGCTGCA GAAGCCGATT
    GACCTAAAGG ACATGGAGTC TGTGGACACG GAGTACTACA ACTCGCTGAT GTGGATTAAG
    GAGAACGATC CACGCATTCT GGAACTGACT TTCTGCCTGG ATGAAGACGT TTTTGGCCAG
    AAGAGTCAGC ACGAACTAAA GCCCGGCGGT GCCAACATAG ACGTGACTAA CGAAAACAAG
    GATGAATACA TTAAATTGGT TATCGAATGG CGCTTTGTGG CACGTGTCAA GGAACAGATG
    TCGTCCTTCC TTGATGGTTT TGGATCGATA ATTCCTCTGA ATCTTATTAA GATTTTCGAC
    GAGCACGAAC TGGAGCTACT GATGTGTGGC ATACAGAACA TCGATGTGAA GGATTGGCGC
    GAGAATACTC TGTACAAGGG CGACTACCAC ATGAATCATA TTATTATTCA GTGGTTCTGG
    AGGGCTGTAC TTTCCTTCTC CAACGAGATG CGTTCTCGTC TGCTGCAATT TGTTACGGGC
    ACTTCACGCG TGCCCATGAA CGGTTTCAAG GAGCTGTACG GCTCAAATGG CCCCCAGATG
    TTTACTATCG AAAAGTGGGG CACTCCTAAT AACTTTCCAA GGGCACATAC CTGTTTCAAT
    CGCCTGGACC TGCCACCCTA TGAAGGCTAC CTGCAACTGA AGGACAAACT GATCAAGGCC
    ATCGAGGGCA GCCAAGGATT TGCTGGAGTT GATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_730284.2
    CDS381..3176
    Translation

    Target ORF information:

    RefSeq Version NM_168738.4
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster Nedd4, transcript variant G (Nedd4), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_168738.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGTCGGCAC GTTCCAGCGG CTTGGTGGCC GCGGCGGCCC TTCCCGTCCC GTCGTCTTCT 
    TCCTCGGTGG CCGGCGGCGA CGTGCCACGC CCGCCGCCCC GCCGGCGAGC GGCCTCTGTT
    GCGGGACAAC AGCAAACGCG CCAGGAATTT GGAAATGGTT ACACTCCTCG CAGATCGTTG
    GCAGCAGTGA ACGACTCTGG GGACTCGTGT CACCTGCGAA TCGTCGTACT CACCGGCCAG
    TCGCTGGCCA AAAAGGATAT ATTCGGCGCC AGCGACCCGT ATGTCAGAAT CGATTTGAAC
    ACAATCAACG GTGATATTAA TATTGACTCT GTTTTGACGA AGACCAAAAA GAAGACACTG
    AATCCAACCT GGAATGAAGA GTTCATATTT AGAGTTAAAC CGTCTGAGCA TAAGCTTGTG
    TTTCAAGTAT TTGACGAGAA TCGCCTGACA CGCGACGACT TCCTGGGCAT GGTGGAGCTG
    ACCCTGGTGA ATCTGCCTAC CGAGCAGGAA GGTCGCACCA TCGGAGAACA AAGCTACACT
    CTGCGCCCGC GCAGGTCAGT AGGCGCCAAA TCCCGCATCA AGGGCACACT GCGCATCTAC
    CACGCCTTTA TCCGGGAGAC GCGGGAGCAG AGCGAGCCAT CTAGTGGCAA TAGCGATGGC
    GAGTGGGAGC ACGTTGAGGC CACCAATGCC GGTGAGACGT CTGCCCAGCC GCACCCCTTT
    CCTACTGGTG GCCACGATGC CCTACCCGCT GGATGGGAAG AACGCCAGGA TGCCAACGGG
    CGCACGTACT ATGTGAATCA CACGGCGAGG ACGACGCAAT GGGATAGGCC TACTGTCTTA
    AACAGCCACA GTAGCCAATC CACTGACGAC CAGTTGGCCT CGGATTTCCA GAGACGTTTC
    CACATCAGCG TGGACGACAC GGAGTCAGGA CGTTCGGCGA GGCCAAGGAA CGCTGCTCAC
    ATGGAGACTG TGGCCAGCGA TGAGAGCAGC GAGGATGAGG ATGTGGCTGA GAATGGCGAG
    GAGATGGTGG ATCTTCAGAA TAATCAGGCC AATTGTCTGC ACTGCGGCGA GCTGCATGAC
    AATGACGAGG CAGAGGAGTA TGTTGATGAG GAGGAGTATA ATGCAGAGCT GCAGCCTCCA
    ACACCTGACC CAGATCAGCC GTCAGCCACA TCCCAAAGCG CGCAAACATG TGATATACTC
    GATAGGAACT TGTACTTGAA ACGCATATTC AATGAAGACA CCGATCACAC AGACAGCCAC
    AATCCCTCTG ACATCTCAGC TCCGTCCACA CGACGCAATT CCGAGGAAGA CAACGCGGCT
    GTGCCTCCCA TGGAACAAAA TACTGGTGGT GAAGAGGAAC CCCTGCCACC GCGCTGGTCG
    ATGCAGGTAG CCCCCAACGG AAGGACATTC TTCATTGATC ATGCGTCCCG GAGGACTACT
    TGGATAGACC CGCGCAACGG ACGGGCCAGC CCTATGCCAA ATCAAACTCG TCGCGTCGAG
    GACGACCTGG GTCCTTTACC AGAGGGCTGG GAGGAGCGAG TACACACCGA CGGACGCGTA
    TTCTATATAG ATCATAACAC GCGAACTACG CAGTGGGAAG ATCCTCGCTT GTCTAATCCA
    AATATTGCCG GTCAGGCTGT GCCCTATTCT CGAGATTACA AGCAAAAATA CGAGTATTTC
    AAGAGTCACA TTAGAAAGCC TACAAATGTA CCAAATAAAT TTGAAATACG TATTCGTCGT
    ACGTCGATAC TGGAGGATTC TTACAGAATT ATTAGTTCGG TAACGAAGAC CGATTTACTA
    AAGACTAAAT TATGGGTAGA GTTTGAAGGA GAAACTGGCT TAGATTATGG AGGCCTCGCA
    AGGGAATGGT TCTATTTATT ATCAAAAGAA ATGTTTAATC CGTACTACGG ACTATTCGAG
    TACTCGGCCA TGGACAACTA CACGCTTCAG ATAAACAATG GTAGTGGTTT GTGCAACGAG
    GAGCATTTAA GTTACTTTAA ATTCATTGGC CGTATTGCGG GCATGGCTGT GTATCATGGA
    AAGCTGCTAG ATGCCTTCTT CATTCGTCCA TTCTACAAGA TGATGCTGCA GAAGCCGATT
    GACCTAAAGG ACATGGAGTC TGTGGACACG GAGTACTACA ACTCGCTGAT GTGGATTAAG
    GAGAACGATC CACGCATTCT GGAACTGACT TTCTGCCTGG ATGAAGACGT TTTTGGCCAG
    AAGAGTCAGC ACGAACTAAA GCCCGGCGGT GCCAACATAG ACGTGACTAA CGAAAACAAG
    GATGAATACA TTAAATTGGT TATCGAATGG CGCTTTGTGG CACGTGTCAA GGAACAGATG
    TCGTCCTTCC TTGATGGTTT TGGATCGATA ATTCCTCTGA ATCTTATTAA GATTTTCGAC
    GAGCACGAAC TGGAGCTACT GATGTGTGGC ATACAGAACA TCGATGTGAA GGATTGGCGC
    GAGAATACTC TGTACAAGGG CGACTACCAC ATGAATCATA TTATTATTCA GTGGTTCTGG
    AGGGCTGTAC TTTCCTTCTC CAACGAGATG CGTTCTCGTC TGCTGCAATT TGTTACGGGC
    ACTTCACGCG TGCCCATGAA CGGTTTCAAG GAGCTGTACG GCTCAAATGG CCCCCAGATG
    TTTACTATCG AAAAGTGGGG CACTCCTAAT AACTTTCCAA GGGCACATAC CTGTTTCAAT
    CGCCTGGACC TGCCACCCTA TGAAGGCTAC CTGCAACTGA AGGACAAACT GATCAAGGCC
    ATCGAGGGCA GCCAAGGATT TGCTGGAGTT GATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa21015
    Clone ID Related Accession (Same CDS sequence) NM_168736.4
    Accession Version NM_168736.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2505bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product Nedd4, isoform K
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_168736.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGTCGGCAC GTTCCAGCGG CTTGGTGGCC GCGGCGGCCC TTCCCGTCCC GTCGTCTTCT 
    TCCTCGGTGG CCGGCGGCGA CGTGCCACGC CCGCCGCCCC GCCGGCGAGC GGCCTCTGTT
    GCGGGACAAC AGCAAACGCG CCAGGAATTT GGAAATGGTT ACACTCCTCG CAGATCGTTG
    GCAGCAGTGA ACGACTCTGG GGACTCGTGT CACCTGCGAA TCGTCGTACT CACCGGCCAG
    TCGCTGGCCA AAAAGGATAT ATTCGGCGCC AGCGACCCGT ATGTCAGAAT CGATTTGAAC
    ACAATCAACG GTGATATTAA TATTGACTCT GTTTTGACGA AGACCAAAAA GAAGACACTG
    AATCCAACCT GGAATGAAGA GTTCATATTT AGAGTTAAAC CGTCTGAGCA TAAGCTTGTG
    TTTCAAGTAT TTGACGAGAA TCGCCTGACA CGCGACGACT TCCTGGGCAT GGTGGAGCTG
    ACCCTGGTGA ATCTGCCTAC CGAGCAGGAA GGTCGCACCA TCGGAGAACA AAGCTACACT
    CTGCGCCCGC GCAGGTCAGT AGGCGCCAAA TCCCGCATCA AGGGCACACT GCGCATCTAC
    CACGCCTTTA TCCGGGAGAC GCGGGAGCAG AGCGAGCCAT CTAGTGGCAA TAGCGATGGC
    GAGTGGGAGC ACGTTGAGGC CACCAATGCC GGTGAGACGT CTGCCCAGCC GCACCCCTTT
    CCTACTGGTG GCCACGATGC CCTACCCGCT GGATGGGAAG AACGCCAGGA TGCCAACGGG
    CGCACGTACT ATGTGAATCA CACGGCGAGG ACGACGCAAT GGGATAGGCC TACTGTCTTA
    AACAGCCACA GTAGCCAATC CACTGACGAC CAGTTGGCCT CGGATTTCCA GAGACGTTTC
    CACATCAGCG TGGACGACAC GGAGTCAGGA CGTTCGGCGA ATGAAGACAC CGATCACACA
    GACAGCCACA ATCCCTCTGA CATCTCAGCT CCGTCCACAC GACGCAATTC CGAGGAAGAC
    AACGCGGCTG TGCCTCCCAT GGAACAAAAT ACTGGTGGTG AAGAGGAACC CCTGCCACCG
    CGCTGGTCGA TGCAGGTAGC CCCCAACGGA AGGACATTCT TCATTGATCA TGCGTCCCGG
    AGGACTACTT GGATAGACCC GCGCAACGGA CGGGCCAGCC CTATGCCAAA TCAAACTCGT
    CGCGTCGAGG ACGACCTGGG TCCTTTACCA GAGGGCTGGG AGGAGCGAGT ACACACCGAC
    GGACGCGTAT TCTATATAGA TCATAACACG CGAACTACGC AGTGGGAAGA TCCTCGCTTG
    TCTAATCCAA ATATTGCCGG TCAGGCTGTG CCCTATTCTC GAGATTACAA GCAAAAATAC
    GAGTATTTCA AGAGTCACAT TAGAAAGCCT ACAAATGTAC CAAATAAATT TGAAATACGT
    ATTCGTCGTA CGTCGATACT GGAGGATTCT TACAGAATTA TTAGTTCGGT AACGAAGACC
    GATTTACTAA AGACTAAATT ATGGGTAGAG TTTGAAGGAG AAACTGGCTT AGATTATGGA
    GGCCTCGCAA GGGAATGGTT CTATTTATTA TCAAAAGAAA TGTTTAATCC GTACTACGGA
    CTATTCGAGT ACTCGGCCAT GGACAACTAC ACGCTTCAGA TAAACAATGG TAGTGGTTTG
    TGCAACGAGG AGCATTTAAG TTACTTTAAA TTCATTGGCC GTATTGCGGG CATGGCTGTG
    TATCATGGAA AGCTGCTAGA TGCCTTCTTC ATTCGTCCAT TCTACAAGAT GATGCTGCAG
    AAGCCGATTG ACCTAAAGGA CATGGAGTCT GTGGACACGG AGTACTACAA CTCGCTGATG
    TGGATTAAGG AGAACGATCC ACGCATTCTG GAACTGACTT TCTGCCTGGA TGAAGACGTT
    TTTGGCCAGA AGAGTCAGCA CGAACTAAAG CCCGGCGGTG CCAACATAGA CGTGACTAAC
    GAAAACAAGG ATGAATACAT TAAATTGGTT ATCGAATGGC GCTTTGTGGC ACGTGTCAAG
    GAACAGATGT CGTCCTTCCT TGATGGTTTT GGATCGATAA TTCCTCTGAA TCTTATTAAG
    ATTTTCGACG AGCACGAACT GGAGCTACTG ATGTGTGGCA TACAGAACAT CGATGTGAAG
    GATTGGCGCG AGAATACTCT GTACAAGGGC GACTACCACA TGAATCATAT TATTATTCAG
    TGGTTCTGGA GGGCTGTACT TTCCTTCTCC AACGAGATGC GTTCTCGTCT GCTGCAATTT
    GTTACGGGCA CTTCACGCGT GCCCATGAAC GGTTTCAAGG AGCTGTACGG CTCAAATGGC
    CCCCAGATGT TTACTATCGA AAAGTGGGGC ACTCCTAATA ACTTTCCAAG GGCACATACC
    TGTTTCAATC GCCTGGACCT GCCACCCTAT GAAGGCTACC TGCAACTGAA GGACAAACTG
    ATCAAGGCCA TCGAGGGCAG CCAAGGATTT GCTGGAGTTG ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_730282.1
    CDS381..2885
    Translation

    Target ORF information:

    RefSeq Version NM_168736.4
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster Nedd4, transcript variant K (Nedd4), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_168736.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGTCGGCAC GTTCCAGCGG CTTGGTGGCC GCGGCGGCCC TTCCCGTCCC GTCGTCTTCT 
    TCCTCGGTGG CCGGCGGCGA CGTGCCACGC CCGCCGCCCC GCCGGCGAGC GGCCTCTGTT
    GCGGGACAAC AGCAAACGCG CCAGGAATTT GGAAATGGTT ACACTCCTCG CAGATCGTTG
    GCAGCAGTGA ACGACTCTGG GGACTCGTGT CACCTGCGAA TCGTCGTACT CACCGGCCAG
    TCGCTGGCCA AAAAGGATAT ATTCGGCGCC AGCGACCCGT ATGTCAGAAT CGATTTGAAC
    ACAATCAACG GTGATATTAA TATTGACTCT GTTTTGACGA AGACCAAAAA GAAGACACTG
    AATCCAACCT GGAATGAAGA GTTCATATTT AGAGTTAAAC CGTCTGAGCA TAAGCTTGTG
    TTTCAAGTAT TTGACGAGAA TCGCCTGACA CGCGACGACT TCCTGGGCAT GGTGGAGCTG
    ACCCTGGTGA ATCTGCCTAC CGAGCAGGAA GGTCGCACCA TCGGAGAACA AAGCTACACT
    CTGCGCCCGC GCAGGTCAGT AGGCGCCAAA TCCCGCATCA AGGGCACACT GCGCATCTAC
    CACGCCTTTA TCCGGGAGAC GCGGGAGCAG AGCGAGCCAT CTAGTGGCAA TAGCGATGGC
    GAGTGGGAGC ACGTTGAGGC CACCAATGCC GGTGAGACGT CTGCCCAGCC GCACCCCTTT
    CCTACTGGTG GCCACGATGC CCTACCCGCT GGATGGGAAG AACGCCAGGA TGCCAACGGG
    CGCACGTACT ATGTGAATCA CACGGCGAGG ACGACGCAAT GGGATAGGCC TACTGTCTTA
    AACAGCCACA GTAGCCAATC CACTGACGAC CAGTTGGCCT CGGATTTCCA GAGACGTTTC
    CACATCAGCG TGGACGACAC GGAGTCAGGA CGTTCGGCGA ATGAAGACAC CGATCACACA
    GACAGCCACA ATCCCTCTGA CATCTCAGCT CCGTCCACAC GACGCAATTC CGAGGAAGAC
    AACGCGGCTG TGCCTCCCAT GGAACAAAAT ACTGGTGGTG AAGAGGAACC CCTGCCACCG
    CGCTGGTCGA TGCAGGTAGC CCCCAACGGA AGGACATTCT TCATTGATCA TGCGTCCCGG
    AGGACTACTT GGATAGACCC GCGCAACGGA CGGGCCAGCC CTATGCCAAA TCAAACTCGT
    CGCGTCGAGG ACGACCTGGG TCCTTTACCA GAGGGCTGGG AGGAGCGAGT ACACACCGAC
    GGACGCGTAT TCTATATAGA TCATAACACG CGAACTACGC AGTGGGAAGA TCCTCGCTTG
    TCTAATCCAA ATATTGCCGG TCAGGCTGTG CCCTATTCTC GAGATTACAA GCAAAAATAC
    GAGTATTTCA AGAGTCACAT TAGAAAGCCT ACAAATGTAC CAAATAAATT TGAAATACGT
    ATTCGTCGTA CGTCGATACT GGAGGATTCT TACAGAATTA TTAGTTCGGT AACGAAGACC
    GATTTACTAA AGACTAAATT ATGGGTAGAG TTTGAAGGAG AAACTGGCTT AGATTATGGA
    GGCCTCGCAA GGGAATGGTT CTATTTATTA TCAAAAGAAA TGTTTAATCC GTACTACGGA
    CTATTCGAGT ACTCGGCCAT GGACAACTAC ACGCTTCAGA TAAACAATGG TAGTGGTTTG
    TGCAACGAGG AGCATTTAAG TTACTTTAAA TTCATTGGCC GTATTGCGGG CATGGCTGTG
    TATCATGGAA AGCTGCTAGA TGCCTTCTTC ATTCGTCCAT TCTACAAGAT GATGCTGCAG
    AAGCCGATTG ACCTAAAGGA CATGGAGTCT GTGGACACGG AGTACTACAA CTCGCTGATG
    TGGATTAAGG AGAACGATCC ACGCATTCTG GAACTGACTT TCTGCCTGGA TGAAGACGTT
    TTTGGCCAGA AGAGTCAGCA CGAACTAAAG CCCGGCGGTG CCAACATAGA CGTGACTAAC
    GAAAACAAGG ATGAATACAT TAAATTGGTT ATCGAATGGC GCTTTGTGGC ACGTGTCAAG
    GAACAGATGT CGTCCTTCCT TGATGGTTTT GGATCGATAA TTCCTCTGAA TCTTATTAAG
    ATTTTCGACG AGCACGAACT GGAGCTACTG ATGTGTGGCA TACAGAACAT CGATGTGAAG
    GATTGGCGCG AGAATACTCT GTACAAGGGC GACTACCACA TGAATCATAT TATTATTCAG
    TGGTTCTGGA GGGCTGTACT TTCCTTCTCC AACGAGATGC GTTCTCGTCT GCTGCAATTT
    GTTACGGGCA CTTCACGCGT GCCCATGAAC GGTTTCAAGG AGCTGTACGG CTCAAATGGC
    CCCCAGATGT TTACTATCGA AAAGTGGGGC ACTCCTAATA ACTTTCCAAG GGCACATACC
    TGTTTCAATC GCCTGGACCT GCCACCCTAT GAAGGCTACC TGCAACTGAA GGACAAACTG
    ATCAAGGCCA TCGAGGGCAG CCAAGGATTT GCTGGAGTTG ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.