NEDD4L cDNA ORF clone, Canis lupus familiaris(dog)

The following NEDD4L gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NEDD4L cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe00763 XM_005615368.3
Latest version!
Canis lupus familiaris neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe00764 XM_005615369.3
Latest version!
Canis lupus familiaris neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe00766 XM_005615370.3
Latest version!
Canis lupus familiaris neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe00768 XM_533393.5
Latest version!
Canis lupus familiaris neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe00769 XM_005615375.2
Latest version!
Canis lupus familiaris neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe285819 XM_022416697.1
Latest version!
Canis lupus familiaris neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OCe34411 XM_014108785.2
Latest version!
Canis lupus familiaris neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OCe34412 XM_014108971.2
Latest version!
Canis lupus familiaris neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OCe34412 XM_014108939.2
Latest version!
Canis lupus familiaris neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OCe34412 XM_014109014.1
Latest version!
Canis lupus familiaris neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OCe34412 XM_014109029.1
Latest version!
Canis lupus familiaris neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
View Old Accession Versions >>
OCe34412 XM_014108971.1 Canis lupus familiaris neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OCe34411 XM_014108785.1 Canis lupus familiaris neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OCe00763 XM_005615368.2 Canis lupus familiaris neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe34412 XM_014108939.1 Canis lupus familiaris neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OCe00764 XM_005615369.2 Canis lupus familiaris neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe00766 XM_005615370.2 Canis lupus familiaris neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe00763
    Clone ID Related Accession (Same CDS sequence) XM_005615368.2 , XM_005615368.3
    Accession Version XM_005615368.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2832bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726046.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005615368.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAGCCTTG AGGACAATTA CAGTGATCCG TATGTGAAAC TTTCATTGTA CGTAGCAGAT 
    GAGAATAGAG AACTCGCTTT GGTACAGACA AAAACCATTA AAAAGACGCT GAACCCAAAG
    TGGAATGAAG AATTTTATTT TAGAGTAAAC CCATCTAATC ACAGACTCCT GTTTGAAGTA
    TTTGATGAAA ACAGATTGAC ACGAGACGAC TTCCTGGGAC AGGTGGACAT ACCCCTTAGT
    CACCTCCCGA CTGAAGATCC GACCATGGAG CGACCCTATA CATTTAAGGA CTTTCTCCTT
    CGACCAAGAA GTCATAAATC TCGAGTTAAG GGATTTTTGC GATTGAAAAT GGCCTATATG
    CCAAAAAATG GCGGTCAAGA TGAAGAAAAC AGTGAACAGA GGGATGATAT GGAGCATGGC
    TGGGAGGTTG TGGACTCGAA TGACTCGGCC TCTCAGCACC AGGAGGAGCT TCCTCCACCC
    CCACTGCCCC CAGGCTGGGA AGAGAAGGTG GACAACCTTG GTCGTACCTA TTATGTCAAC
    CACAACAACC GGACCACCCA GTGGCACCGA CCAAGCTTGA TGGATGTGTC CTCCGAGTCC
    GACAGCAACA TCAGGCAGAT CAACCAGGAG GCCGCGCACC GGCGCTTCCG CTCACGCAGG
    CACATCAGCG AGGACCTGGA GCCCGAGCCT GGCGAGGGTG GAGACGGCCC AGAGCCTTGG
    GAGACCATCT CCGAAGAAGT GAACATCACG GGAGACCCCC TCAGTCTGGC GCTGCCCCCA
    CCACCATCCT CTCCAGTATC TCGGACCAGC CCTCAGGAGC TGTCTGAGGA ACTGAGCAGA
    AGGCTTCAGA TCACTCCAGA CTCAAATGGG GAGCAATTTG GTTCTTTGAT GCAAAGAGAG
    CCCTCCTCAA GGCTGCGGTC CTGCAGTGTC ACCGACGGGG TCGCCGAGCA GGCTCATCTA
    CCACCGCCGA GTGTCCCAAC TAGGAGAGCG CGGTCATCCA CCGTCACGGG TGGTGAGGAG
    CCAACGCCAT CAGTGGCCTA TGCACACACC ACGCCGGGCC TACCTTCAGG CTGGGAAGAA
    AGAAAAGATG CTAAGGGACG CACATACTAT GTCAATCATA ACAATCGAAC CACAACTTGG
    ACTCGACCTA TCATGCAGCT CGCCGAAGAC GGTGCGTCCG GATCCGCCAC AAACAGTAAC
    AACCACCTAA TCGAGCCTCA GATCCGCCGG CCCCGTAGCC TCAGCTCGCC GACCGTAACC
    TTATCTGCCC CACTGGAGGG TGCCAAGGAT TCGCCCATAC GTCGGGCTGT GAAAGATACC
    CTTTCCAATC CACAGTCCCC ACAGCCATCA CCTTACAACT CCCCCAAACC ACAACACAAA
    GTCACACAGA GCTTCCTGCC GCCCGGCTGG GAAATGAGGA TAGCGCCCAA CGGCCGGCCC
    TTCTTCATTG ACCATAACAC AAAGACTACA ACGTGGGAAG ATCCACGCCT GAAATTTCCA
    GTACATATGC GGTCAAAGGC ATCCTTAAAC CCCAATGACC TTGGCCCTCT TCCTCCTGGT
    TGGGAAGAAA GAATTCACTT GGATGGCCGA ACGTTTTATA TTGACCATAA TAGCAAAATT
    ACTCAGTGGG AAGATCCAAG ACTGCAGAAT CCAGCTATTA CTGGTCCGAA GGCTGTTCCT
    TACTCCAGAG AATTTAAGCA GAAATATGAC TACTTTAGGA AGAAATTAAA GAAACCTGCT
    GATATTCCAA ATAGGTTTGA AATGAAACTT CACAGAAATA ACATCTTTGA AGAGTCCTAT
    CGGAGAATCA TGTCTGTGAA AAGACCGGAT GTCCTGAAAG CTAGACTGTG GATCGAATTT
    GAATCAGAGA AAGGTCTTGA CTATGGGGGT GTGGCCAGAG AGTGGTTCTT CCTCCTGTCC
    AAAGAGATGT TCAACCCCTA CTACGGGCTT TTTGAGTACT CTGCAACGGA CAACTACACA
    CTTCAGATCA ATCCCAATTC AGGCCTCTGT AATGAAGACC ATTTGTCCTA TTTCACTTTT
    ATTGGAAGAG TTGCTGGTCT GGCAGTATTT CATGGGAAAC TTCTAGATGG TTTCTTCATT
    AGACCGTTTT ACAAAATGAT GTTGGGAAAG CAGATAACTC TGAATGACAT GGAATCTGTG
    GATAGTGAAT ATTACAACTC TTTGAAATGG ATCTTGGAAA ATGATCCTAC TGAACTGGAC
    CTCATGTTCT GTATCGATGA AGAAAACTTT GGGCAGACCT ATCAAGTGGA TCTGAAGCCC
    AATGGGTCAG AAATAATGGT GACAAATGAA AACAAGAGAG AATATATTGA CTTAGTCATC
    CAGTGGAGGT TTGTGAACAG GGTCCAGAAG CAGATGAACG CCTTCTTGGA GGGATTCACA
    GAACTGCTTC CTATTGATTT GATTAAAATT TTCGATGAAA ATGAGCTGGA GTTGCTGATG
    TGCGGCCTTG GCGATGTGGA CGTGAACGAT TGGAGACAGC ATTCTATTTA CAAGAATGGC
    TACTGCCCCA ACCACCCTGT CATTCAGTGG TTCTGGAAGG CTGTGCTGCT CATGGACGCG
    GAGAAGCGGA TCCGGCTACT GCAGTTCGTC ACGGGGACGT CCCGGGTACC TATGAACGGA
    TTCGCCGAAC TTTATGGTTC CAATGGTCCT CAGCTGTTTA CAATAGAGCA ATGGGGGAGT
    CCTGACAAGC TGCCCAGAGC TCACACGTGC TTTAATCGCC TTGACTTACC TCCATATGAA
    ACCTTTGAAG ATTTACGAGA GAAACTCCTC ATGGCTGTGG AAAACGCTCA AGGATTTGAG
    GGGGTGGATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005615425.1
    CDS223..3054
    Translation

    Target ORF information:

    RefSeq Version XM_005615368.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005615368.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAGCCTTG AGGACAATTA CAGTGATCCG TATGTGAAAC TTTCATTGTA CGTAGCAGAT 
    GAGAATAGAG AACTCGCTTT GGTACAGACA AAAACCATTA AAAAGACGCT GAACCCAAAG
    TGGAATGAAG AATTTTATTT TAGAGTAAAC CCATCTAATC ACAGACTCCT GTTTGAAGTA
    TTTGATGAAA ACAGATTGAC ACGAGACGAC TTCCTGGGAC AGGTGGACAT ACCCCTTAGT
    CACCTCCCGA CTGAAGATCC GACCATGGAG CGACCCTATA CATTTAAGGA CTTTCTCCTT
    CGACCAAGAA GTCATAAATC TCGAGTTAAG GGATTTTTGC GATTGAAAAT GGCCTATATG
    CCAAAAAATG GCGGTCAAGA TGAAGAAAAC AGTGAACAGA GGGATGATAT GGAGCATGGC
    TGGGAGGTTG TGGACTCGAA TGACTCGGCC TCTCAGCACC AGGAGGAGCT TCCTCCACCC
    CCACTGCCCC CAGGCTGGGA AGAGAAGGTG GACAACCTTG GTCGTACCTA TTATGTCAAC
    CACAACAACC GGACCACCCA GTGGCACCGA CCAAGCTTGA TGGATGTGTC CTCCGAGTCC
    GACAGCAACA TCAGGCAGAT CAACCAGGAG GCCGCGCACC GGCGCTTCCG CTCACGCAGG
    CACATCAGCG AGGACCTGGA GCCCGAGCCT GGCGAGGGTG GAGACGGCCC AGAGCCTTGG
    GAGACCATCT CCGAAGAAGT GAACATCACG GGAGACCCCC TCAGTCTGGC GCTGCCCCCA
    CCACCATCCT CTCCAGTATC TCGGACCAGC CCTCAGGAGC TGTCTGAGGA ACTGAGCAGA
    AGGCTTCAGA TCACTCCAGA CTCAAATGGG GAGCAATTTG GTTCTTTGAT GCAAAGAGAG
    CCCTCCTCAA GGCTGCGGTC CTGCAGTGTC ACCGACGGGG TCGCCGAGCA GGCTCATCTA
    CCACCGCCGA GTGTCCCAAC TAGGAGAGCG CGGTCATCCA CCGTCACGGG TGGTGAGGAG
    CCAACGCCAT CAGTGGCCTA TGCACACACC ACGCCGGGCC TACCTTCAGG CTGGGAAGAA
    AGAAAAGATG CTAAGGGACG CACATACTAT GTCAATCATA ACAATCGAAC CACAACTTGG
    ACTCGACCTA TCATGCAGCT CGCCGAAGAC GGTGCGTCCG GATCCGCCAC AAACAGTAAC
    AACCACCTAA TCGAGCCTCA GATCCGCCGG CCCCGTAGCC TCAGCTCGCC GACCGTAACC
    TTATCTGCCC CACTGGAGGG TGCCAAGGAT TCGCCCATAC GTCGGGCTGT GAAAGATACC
    CTTTCCAATC CACAGTCCCC ACAGCCATCA CCTTACAACT CCCCCAAACC ACAACACAAA
    GTCACACAGA GCTTCCTGCC GCCCGGCTGG GAAATGAGGA TAGCGCCCAA CGGCCGGCCC
    TTCTTCATTG ACCATAACAC AAAGACTACA ACGTGGGAAG ATCCACGCCT GAAATTTCCA
    GTACATATGC GGTCAAAGGC ATCCTTAAAC CCCAATGACC TTGGCCCTCT TCCTCCTGGT
    TGGGAAGAAA GAATTCACTT GGATGGCCGA ACGTTTTATA TTGACCATAA TAGCAAAATT
    ACTCAGTGGG AAGATCCAAG ACTGCAGAAT CCAGCTATTA CTGGTCCGAA GGCTGTTCCT
    TACTCCAGAG AATTTAAGCA GAAATATGAC TACTTTAGGA AGAAATTAAA GAAACCTGCT
    GATATTCCAA ATAGGTTTGA AATGAAACTT CACAGAAATA ACATCTTTGA AGAGTCCTAT
    CGGAGAATCA TGTCTGTGAA AAGACCGGAT GTCCTGAAAG CTAGACTGTG GATCGAATTT
    GAATCAGAGA AAGGTCTTGA CTATGGGGGT GTGGCCAGAG AGTGGTTCTT CCTCCTGTCC
    AAAGAGATGT TCAACCCCTA CTACGGGCTT TTTGAGTACT CTGCAACGGA CAACTACACA
    CTTCAGATCA ATCCCAATTC AGGCCTCTGT AATGAAGACC ATTTGTCCTA TTTCACTTTT
    ATTGGAAGAG TTGCTGGTCT GGCAGTATTT CATGGGAAAC TTCTAGATGG TTTCTTCATT
    AGACCGTTTT ACAAAATGAT GTTGGGAAAG CAGATAACTC TGAATGACAT GGAATCTGTG
    GATAGTGAAT ATTACAACTC TTTGAAATGG ATCTTGGAAA ATGATCCTAC TGAACTGGAC
    CTCATGTTCT GTATCGATGA AGAAAACTTT GGGCAGACCT ATCAAGTGGA TCTGAAGCCC
    AATGGGTCAG AAATAATGGT GACAAATGAA AACAAGAGAG AATATATTGA CTTAGTCATC
    CAGTGGAGGT TTGTGAACAG GGTCCAGAAG CAGATGAACG CCTTCTTGGA GGGATTCACA
    GAACTGCTTC CTATTGATTT GATTAAAATT TTCGATGAAA ATGAGCTGGA GTTGCTGATG
    TGCGGCCTTG GCGATGTGGA CGTGAACGAT TGGAGACAGC ATTCTATTTA CAAGAATGGC
    TACTGCCCCA ACCACCCTGT CATTCAGTGG TTCTGGAAGG CTGTGCTGCT CATGGACGCG
    GAGAAGCGGA TCCGGCTACT GCAGTTCGTC ACGGGGACGT CCCGGGTACC TATGAACGGA
    TTCGCCGAAC TTTATGGTTC CAATGGTCCT CAGCTGTTTA CAATAGAGCA ATGGGGGAGT
    CCTGACAAGC TGCCCAGAGC TCACACGTGC TTTAATCGCC TTGACTTACC TCCATATGAA
    ACCTTTGAAG ATTTACGAGA GAAACTCCTC ATGGCTGTGG AAAACGCTCA AGGATTTGAG
    GGGGTGGATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe00763
    Clone ID Related Accession (Same CDS sequence) XM_005615368.2 , XM_005615368.3
    Accession Version XM_005615368.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2832bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005615368.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAGCCTTG AGGACAATTA CAGTGATCCG TATGTGAAAC TTTCATTGTA CGTAGCAGAT 
    GAGAATAGAG AACTCGCTTT GGTACAGACA AAAACCATTA AAAAGACGCT GAACCCAAAG
    TGGAATGAAG AATTTTATTT TAGAGTAAAC CCATCTAATC ACAGACTCCT GTTTGAAGTA
    TTTGATGAAA ACAGATTGAC ACGAGACGAC TTCCTGGGAC AGGTGGACAT ACCCCTTAGT
    CACCTCCCGA CTGAAGATCC GACCATGGAG CGACCCTATA CATTTAAGGA CTTTCTCCTT
    CGACCAAGAA GTCATAAATC TCGAGTTAAG GGATTTTTGC GATTGAAAAT GGCCTATATG
    CCAAAAAATG GCGGTCAAGA TGAAGAAAAC AGTGAACAGA GGGATGATAT GGAGCATGGC
    TGGGAGGTTG TGGACTCGAA TGACTCGGCC TCTCAGCACC AGGAGGAGCT TCCTCCACCC
    CCACTGCCCC CAGGCTGGGA AGAGAAGGTG GACAACCTTG GTCGTACCTA TTATGTCAAC
    CACAACAACC GGACCACCCA GTGGCACCGA CCAAGCTTGA TGGATGTGTC CTCCGAGTCC
    GACAGCAACA TCAGGCAGAT CAACCAGGAG GCCGCGCACC GGCGCTTCCG CTCACGCAGG
    CACATCAGCG AGGACCTGGA GCCCGAGCCT GGCGAGGGTG GAGACGGCCC AGAGCCTTGG
    GAGACCATCT CCGAAGAAGT GAACATCACG GGAGACCCCC TCAGTCTGGC GCTGCCCCCA
    CCACCATCCT CTCCAGTATC TCGGACCAGC CCTCAGGAGC TGTCTGAGGA ACTGAGCAGA
    AGGCTTCAGA TCACTCCAGA CTCAAATGGG GAGCAATTTG GTTCTTTGAT GCAAAGAGAG
    CCCTCCTCAA GGCTGCGGTC CTGCAGTGTC ACCGACGGGG TCGCCGAGCA GGCTCATCTA
    CCACCGCCGA GTGTCCCAAC TAGGAGAGCG CGGTCATCCA CCGTCACGGG TGGTGAGGAG
    CCAACGCCAT CAGTGGCCTA TGCACACACC ACGCCGGGCC TACCTTCAGG CTGGGAAGAA
    AGAAAAGATG CTAAGGGACG CACATACTAT GTCAATCATA ACAATCGAAC CACAACTTGG
    ACTCGACCTA TCATGCAGCT CGCCGAAGAC GGTGCGTCCG GATCCGCCAC AAACAGTAAC
    AACCACCTAA TCGAGCCTCA GATCCGCCGG CCCCGTAGCC TCAGCTCGCC GACCGTAACC
    TTATCTGCCC CACTGGAGGG TGCCAAGGAT TCGCCCATAC GTCGGGCTGT GAAAGATACC
    CTTTCCAATC CACAGTCCCC ACAGCCATCA CCTTACAACT CCCCCAAACC ACAACACAAA
    GTCACACAGA GCTTCCTGCC GCCCGGCTGG GAAATGAGGA TAGCGCCCAA CGGCCGGCCC
    TTCTTCATTG ACCATAACAC AAAGACTACA ACGTGGGAAG ATCCACGCCT GAAATTTCCA
    GTACATATGC GGTCAAAGGC ATCCTTAAAC CCCAATGACC TTGGCCCTCT TCCTCCTGGT
    TGGGAAGAAA GAATTCACTT GGATGGCCGA ACGTTTTATA TTGACCATAA TAGCAAAATT
    ACTCAGTGGG AAGATCCAAG ACTGCAGAAT CCAGCTATTA CTGGTCCGAA GGCTGTTCCT
    TACTCCAGAG AATTTAAGCA GAAATATGAC TACTTTAGGA AGAAATTAAA GAAACCTGCT
    GATATTCCAA ATAGGTTTGA AATGAAACTT CACAGAAATA ACATCTTTGA AGAGTCCTAT
    CGGAGAATCA TGTCTGTGAA AAGACCGGAT GTCCTGAAAG CTAGACTGTG GATCGAATTT
    GAATCAGAGA AAGGTCTTGA CTATGGGGGT GTGGCCAGAG AGTGGTTCTT CCTCCTGTCC
    AAAGAGATGT TCAACCCCTA CTACGGGCTT TTTGAGTACT CTGCAACGGA CAACTACACA
    CTTCAGATCA ATCCCAATTC AGGCCTCTGT AATGAAGACC ATTTGTCCTA TTTCACTTTT
    ATTGGAAGAG TTGCTGGTCT GGCAGTATTT CATGGGAAAC TTCTAGATGG TTTCTTCATT
    AGACCGTTTT ACAAAATGAT GTTGGGAAAG CAGATAACTC TGAATGACAT GGAATCTGTG
    GATAGTGAAT ATTACAACTC TTTGAAATGG ATCTTGGAAA ATGATCCTAC TGAACTGGAC
    CTCATGTTCT GTATCGATGA AGAAAACTTT GGGCAGACCT ATCAAGTGGA TCTGAAGCCC
    AATGGGTCAG AAATAATGGT GACAAATGAA AACAAGAGAG AATATATTGA CTTAGTCATC
    CAGTGGAGGT TTGTGAACAG GGTCCAGAAG CAGATGAACG CCTTCTTGGA GGGATTCACA
    GAACTGCTTC CTATTGATTT GATTAAAATT TTCGATGAAA ATGAGCTGGA GTTGCTGATG
    TGCGGCCTTG GCGATGTGGA CGTGAACGAT TGGAGACAGC ATTCTATTTA CAAGAATGGC
    TACTGCCCCA ACCACCCTGT CATTCAGTGG TTCTGGAAGG CTGTGCTGCT CATGGACGCG
    GAGAAGCGGA TCCGGCTACT GCAGTTCGTC ACGGGGACGT CCCGGGTACC TATGAACGGA
    TTCGCCGAAC TTTATGGTTC CAATGGTCCT CAGCTGTTTA CAATAGAGCA ATGGGGGAGT
    CCTGACAAGC TGCCCAGAGC TCACACGTGC TTTAATCGCC TTGACTTACC TCCATATGAA
    ACCTTTGAAG ATTTACGAGA GAAACTCCTC ATGGCTGTGG AAAACGCTCA AGGATTTGAG
    GGGGTGGATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005615425.1
    CDS208..3039
    Translation

    Target ORF information:

    RefSeq Version XM_005615368.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005615368.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAGCCTTG AGGACAATTA CAGTGATCCG TATGTGAAAC TTTCATTGTA CGTAGCAGAT 
    GAGAATAGAG AACTCGCTTT GGTACAGACA AAAACCATTA AAAAGACGCT GAACCCAAAG
    TGGAATGAAG AATTTTATTT TAGAGTAAAC CCATCTAATC ACAGACTCCT GTTTGAAGTA
    TTTGATGAAA ACAGATTGAC ACGAGACGAC TTCCTGGGAC AGGTGGACAT ACCCCTTAGT
    CACCTCCCGA CTGAAGATCC GACCATGGAG CGACCCTATA CATTTAAGGA CTTTCTCCTT
    CGACCAAGAA GTCATAAATC TCGAGTTAAG GGATTTTTGC GATTGAAAAT GGCCTATATG
    CCAAAAAATG GCGGTCAAGA TGAAGAAAAC AGTGAACAGA GGGATGATAT GGAGCATGGC
    TGGGAGGTTG TGGACTCGAA TGACTCGGCC TCTCAGCACC AGGAGGAGCT TCCTCCACCC
    CCACTGCCCC CAGGCTGGGA AGAGAAGGTG GACAACCTTG GTCGTACCTA TTATGTCAAC
    CACAACAACC GGACCACCCA GTGGCACCGA CCAAGCTTGA TGGATGTGTC CTCCGAGTCC
    GACAGCAACA TCAGGCAGAT CAACCAGGAG GCCGCGCACC GGCGCTTCCG CTCACGCAGG
    CACATCAGCG AGGACCTGGA GCCCGAGCCT GGCGAGGGTG GAGACGGCCC AGAGCCTTGG
    GAGACCATCT CCGAAGAAGT GAACATCACG GGAGACCCCC TCAGTCTGGC GCTGCCCCCA
    CCACCATCCT CTCCAGTATC TCGGACCAGC CCTCAGGAGC TGTCTGAGGA ACTGAGCAGA
    AGGCTTCAGA TCACTCCAGA CTCAAATGGG GAGCAATTTG GTTCTTTGAT GCAAAGAGAG
    CCCTCCTCAA GGCTGCGGTC CTGCAGTGTC ACCGACGGGG TCGCCGAGCA GGCTCATCTA
    CCACCGCCGA GTGTCCCAAC TAGGAGAGCG CGGTCATCCA CCGTCACGGG TGGTGAGGAG
    CCAACGCCAT CAGTGGCCTA TGCACACACC ACGCCGGGCC TACCTTCAGG CTGGGAAGAA
    AGAAAAGATG CTAAGGGACG CACATACTAT GTCAATCATA ACAATCGAAC CACAACTTGG
    ACTCGACCTA TCATGCAGCT CGCCGAAGAC GGTGCGTCCG GATCCGCCAC AAACAGTAAC
    AACCACCTAA TCGAGCCTCA GATCCGCCGG CCCCGTAGCC TCAGCTCGCC GACCGTAACC
    TTATCTGCCC CACTGGAGGG TGCCAAGGAT TCGCCCATAC GTCGGGCTGT GAAAGATACC
    CTTTCCAATC CACAGTCCCC ACAGCCATCA CCTTACAACT CCCCCAAACC ACAACACAAA
    GTCACACAGA GCTTCCTGCC GCCCGGCTGG GAAATGAGGA TAGCGCCCAA CGGCCGGCCC
    TTCTTCATTG ACCATAACAC AAAGACTACA ACGTGGGAAG ATCCACGCCT GAAATTTCCA
    GTACATATGC GGTCAAAGGC ATCCTTAAAC CCCAATGACC TTGGCCCTCT TCCTCCTGGT
    TGGGAAGAAA GAATTCACTT GGATGGCCGA ACGTTTTATA TTGACCATAA TAGCAAAATT
    ACTCAGTGGG AAGATCCAAG ACTGCAGAAT CCAGCTATTA CTGGTCCGAA GGCTGTTCCT
    TACTCCAGAG AATTTAAGCA GAAATATGAC TACTTTAGGA AGAAATTAAA GAAACCTGCT
    GATATTCCAA ATAGGTTTGA AATGAAACTT CACAGAAATA ACATCTTTGA AGAGTCCTAT
    CGGAGAATCA TGTCTGTGAA AAGACCGGAT GTCCTGAAAG CTAGACTGTG GATCGAATTT
    GAATCAGAGA AAGGTCTTGA CTATGGGGGT GTGGCCAGAG AGTGGTTCTT CCTCCTGTCC
    AAAGAGATGT TCAACCCCTA CTACGGGCTT TTTGAGTACT CTGCAACGGA CAACTACACA
    CTTCAGATCA ATCCCAATTC AGGCCTCTGT AATGAAGACC ATTTGTCCTA TTTCACTTTT
    ATTGGAAGAG TTGCTGGTCT GGCAGTATTT CATGGGAAAC TTCTAGATGG TTTCTTCATT
    AGACCGTTTT ACAAAATGAT GTTGGGAAAG CAGATAACTC TGAATGACAT GGAATCTGTG
    GATAGTGAAT ATTACAACTC TTTGAAATGG ATCTTGGAAA ATGATCCTAC TGAACTGGAC
    CTCATGTTCT GTATCGATGA AGAAAACTTT GGGCAGACCT ATCAAGTGGA TCTGAAGCCC
    AATGGGTCAG AAATAATGGT GACAAATGAA AACAAGAGAG AATATATTGA CTTAGTCATC
    CAGTGGAGGT TTGTGAACAG GGTCCAGAAG CAGATGAACG CCTTCTTGGA GGGATTCACA
    GAACTGCTTC CTATTGATTT GATTAAAATT TTCGATGAAA ATGAGCTGGA GTTGCTGATG
    TGCGGCCTTG GCGATGTGGA CGTGAACGAT TGGAGACAGC ATTCTATTTA CAAGAATGGC
    TACTGCCCCA ACCACCCTGT CATTCAGTGG TTCTGGAAGG CTGTGCTGCT CATGGACGCG
    GAGAAGCGGA TCCGGCTACT GCAGTTCGTC ACGGGGACGT CCCGGGTACC TATGAACGGA
    TTCGCCGAAC TTTATGGTTC CAATGGTCCT CAGCTGTTTA CAATAGAGCA ATGGGGGAGT
    CCTGACAAGC TGCCCAGAGC TCACACGTGC TTTAATCGCC TTGACTTACC TCCATATGAA
    ACCTTTGAAG ATTTACGAGA GAAACTCCTC ATGGCTGTGG AAAACGCTCA AGGATTTGAG
    GGGGTGGATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe00764
    Clone ID Related Accession (Same CDS sequence) XM_005615369.2 , XM_005615369.3
    Accession Version XM_005615369.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2829bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726046.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005615369.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAGCCTTG AGGACAATTA CAGTGATCCG TATGTGAAAC TTTCATTGTA CGTAGCAGAT 
    GAGAATAGAG AACTCGCTTT GGTACAGACA AAAACCATTA AAAAGACGCT GAACCCAAAG
    TGGAATGAAG AATTTTATTT TAGAGTAAAC CCATCTAATC ACAGACTCCT GTTTGAAGTA
    TTTGATGAAA ACAGATTGAC ACGAGACGAC TTCCTGGGAC AGGTGGACAT ACCCCTTAGT
    CACCTCCCGA CTGAAGATCC GACCATGGAG CGACCCTATA CATTTAAGGA CTTTCTCCTT
    CGACCAAGAA GTCATAAATC TCGAGTTAAG GGATTTTTGC GATTGAAAAT GGCCTATATG
    CCAAAAAATG GCGGTCAAGA TGAAGAAAAC AGTGAACAGA GGGATGATAT GGAGCATGGC
    TGGGAGGTTG TGGACTCGAA TGACTCGGCC TCTCAGCACC AGGAGGAGCT TCCTCCACCC
    CCACTGCCCC CAGGCTGGGA AGAGAAGGTG GACAACCTTG GTCGTACCTA TTATGTCAAC
    CACAACAACC GGACCACCCA GTGGCACCGA CCAAGCTTGA TGGATGTGTC CTCCGAGTCC
    GACAGCAACA TCAGGCAGAT CAACCAGGAG GCCGCGCACC GGCGCTTCCG CTCACGCAGG
    CACATCAGCG AGGACCTGGA GCCCGAGCCT GGCGAGGGTG GAGACGGCCC AGAGCCTTGG
    GAGACCATCT CCGAAGAAGT GAACATCACG GGAGACCCCC TCAGTCTGGC GCTGCCCCCA
    CCACCATCCT CTCCAGTATC TCGGACCAGC CCTCAGGAGC TGTCTGAGGA ACTGAGCAGA
    AGGCTTCAGA TCACTCCAGA CTCAAATGGG GAGCAATTTG GTTCTTTGAT GCAAAGAGAG
    CCCTCCTCAA GGCTGCGGTC CTGCAGTGTC ACCGACGGGG TCGCCGAGCA GGCTCATCTA
    CCACCGCCGA GTGTCCCAAC TAGGAGAGCG CGGTCATCCA CCGTCACGGG TGGTGAGGAG
    CCAACGCCAT CAGTGGCCTA TGCACACACC ACGCCGGGCC TACCTTCAGG CTGGGAAGAA
    AGAAAAGATG CTAAGGGACG CACATACTAT GTCAATCATA ACAATCGAAC CACAACTTGG
    ACTCGACCTA TCATGCAGCT CGCCGAAGAC GGTGCGTCCG GATCCGCCAC AAACAGTAAC
    AACCACCTAA TCGAGCCTCA GATCCGCCGG CCCCGTAGCC TCAGCTCGCC GACCGTAACC
    TTATCTGCCC CACTGGAGGG TGCCAAGGAT TCGCCCATAC GTCGGGCTGT GAAAGATACC
    CTTTCCAATC CACAGTCCCC ACAGCCATCA CCTTACAACT CCCCCAAACC ACAACACAAA
    GTCACACAGA GCTTCCTGCC GCCCGGCTGG GAAATGAGGA TAGCGCCCAA CGGCCGGCCC
    TTCTTCATTG ACCATAACAC AAAGACTACA ACGTGGGAAG ATCCACGCCT GAAATTTCCA
    GTACATATGC GGTCAAAGGC ATCCTTAAAC CCCAATGACC TTGGCCCTCT TCCTCCTGGT
    TGGGAAGAAA GAATTCACTT GGATGGCCGA ACGTTTTATA TTGACCATAA TAGCAAAATT
    ACTCAGTGGG AAGATCCAAG ACTGCAGAAT CCAGCTATTA CTGGTCCGGC TGTTCCTTAC
    TCCAGAGAAT TTAAGCAGAA ATATGACTAC TTTAGGAAGA AATTAAAGAA ACCTGCTGAT
    ATTCCAAATA GGTTTGAAAT GAAACTTCAC AGAAATAACA TCTTTGAAGA GTCCTATCGG
    AGAATCATGT CTGTGAAAAG ACCGGATGTC CTGAAAGCTA GACTGTGGAT CGAATTTGAA
    TCAGAGAAAG GTCTTGACTA TGGGGGTGTG GCCAGAGAGT GGTTCTTCCT CCTGTCCAAA
    GAGATGTTCA ACCCCTACTA CGGGCTTTTT GAGTACTCTG CAACGGACAA CTACACACTT
    CAGATCAATC CCAATTCAGG CCTCTGTAAT GAAGACCATT TGTCCTATTT CACTTTTATT
    GGAAGAGTTG CTGGTCTGGC AGTATTTCAT GGGAAACTTC TAGATGGTTT CTTCATTAGA
    CCGTTTTACA AAATGATGTT GGGAAAGCAG ATAACTCTGA ATGACATGGA ATCTGTGGAT
    AGTGAATATT ACAACTCTTT GAAATGGATC TTGGAAAATG ATCCTACTGA ACTGGACCTC
    ATGTTCTGTA TCGATGAAGA AAACTTTGGG CAGACCTATC AAGTGGATCT GAAGCCCAAT
    GGGTCAGAAA TAATGGTGAC AAATGAAAAC AAGAGAGAAT ATATTGACTT AGTCATCCAG
    TGGAGGTTTG TGAACAGGGT CCAGAAGCAG ATGAACGCCT TCTTGGAGGG ATTCACAGAA
    CTGCTTCCTA TTGATTTGAT TAAAATTTTC GATGAAAATG AGCTGGAGTT GCTGATGTGC
    GGCCTTGGCG ATGTGGACGT GAACGATTGG AGACAGCATT CTATTTACAA GAATGGCTAC
    TGCCCCAACC ACCCTGTCAT TCAGTGGTTC TGGAAGGCTG TGCTGCTCAT GGACGCGGAG
    AAGCGGATCC GGCTACTGCA GTTCGTCACG GGGACGTCCC GGGTACCTAT GAACGGATTC
    GCCGAACTTT ATGGTTCCAA TGGTCCTCAG CTGTTTACAA TAGAGCAATG GGGGAGTCCT
    GACAAGCTGC CCAGAGCTCA CACGTGCTTT AATCGCCTTG ACTTACCTCC ATATGAAACC
    TTTGAAGATT TACGAGAGAA ACTCCTCATG GCTGTGGAAA ACGCTCAAGG ATTTGAGGGG
    GTGGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005615426.1
    CDS223..3051
    Translation

    Target ORF information:

    RefSeq Version XM_005615369.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005615369.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAGCCTTG AGGACAATTA CAGTGATCCG TATGTGAAAC TTTCATTGTA CGTAGCAGAT 
    GAGAATAGAG AACTCGCTTT GGTACAGACA AAAACCATTA AAAAGACGCT GAACCCAAAG
    TGGAATGAAG AATTTTATTT TAGAGTAAAC CCATCTAATC ACAGACTCCT GTTTGAAGTA
    TTTGATGAAA ACAGATTGAC ACGAGACGAC TTCCTGGGAC AGGTGGACAT ACCCCTTAGT
    CACCTCCCGA CTGAAGATCC GACCATGGAG CGACCCTATA CATTTAAGGA CTTTCTCCTT
    CGACCAAGAA GTCATAAATC TCGAGTTAAG GGATTTTTGC GATTGAAAAT GGCCTATATG
    CCAAAAAATG GCGGTCAAGA TGAAGAAAAC AGTGAACAGA GGGATGATAT GGAGCATGGC
    TGGGAGGTTG TGGACTCGAA TGACTCGGCC TCTCAGCACC AGGAGGAGCT TCCTCCACCC
    CCACTGCCCC CAGGCTGGGA AGAGAAGGTG GACAACCTTG GTCGTACCTA TTATGTCAAC
    CACAACAACC GGACCACCCA GTGGCACCGA CCAAGCTTGA TGGATGTGTC CTCCGAGTCC
    GACAGCAACA TCAGGCAGAT CAACCAGGAG GCCGCGCACC GGCGCTTCCG CTCACGCAGG
    CACATCAGCG AGGACCTGGA GCCCGAGCCT GGCGAGGGTG GAGACGGCCC AGAGCCTTGG
    GAGACCATCT CCGAAGAAGT GAACATCACG GGAGACCCCC TCAGTCTGGC GCTGCCCCCA
    CCACCATCCT CTCCAGTATC TCGGACCAGC CCTCAGGAGC TGTCTGAGGA ACTGAGCAGA
    AGGCTTCAGA TCACTCCAGA CTCAAATGGG GAGCAATTTG GTTCTTTGAT GCAAAGAGAG
    CCCTCCTCAA GGCTGCGGTC CTGCAGTGTC ACCGACGGGG TCGCCGAGCA GGCTCATCTA
    CCACCGCCGA GTGTCCCAAC TAGGAGAGCG CGGTCATCCA CCGTCACGGG TGGTGAGGAG
    CCAACGCCAT CAGTGGCCTA TGCACACACC ACGCCGGGCC TACCTTCAGG CTGGGAAGAA
    AGAAAAGATG CTAAGGGACG CACATACTAT GTCAATCATA ACAATCGAAC CACAACTTGG
    ACTCGACCTA TCATGCAGCT CGCCGAAGAC GGTGCGTCCG GATCCGCCAC AAACAGTAAC
    AACCACCTAA TCGAGCCTCA GATCCGCCGG CCCCGTAGCC TCAGCTCGCC GACCGTAACC
    TTATCTGCCC CACTGGAGGG TGCCAAGGAT TCGCCCATAC GTCGGGCTGT GAAAGATACC
    CTTTCCAATC CACAGTCCCC ACAGCCATCA CCTTACAACT CCCCCAAACC ACAACACAAA
    GTCACACAGA GCTTCCTGCC GCCCGGCTGG GAAATGAGGA TAGCGCCCAA CGGCCGGCCC
    TTCTTCATTG ACCATAACAC AAAGACTACA ACGTGGGAAG ATCCACGCCT GAAATTTCCA
    GTACATATGC GGTCAAAGGC ATCCTTAAAC CCCAATGACC TTGGCCCTCT TCCTCCTGGT
    TGGGAAGAAA GAATTCACTT GGATGGCCGA ACGTTTTATA TTGACCATAA TAGCAAAATT
    ACTCAGTGGG AAGATCCAAG ACTGCAGAAT CCAGCTATTA CTGGTCCGGC TGTTCCTTAC
    TCCAGAGAAT TTAAGCAGAA ATATGACTAC TTTAGGAAGA AATTAAAGAA ACCTGCTGAT
    ATTCCAAATA GGTTTGAAAT GAAACTTCAC AGAAATAACA TCTTTGAAGA GTCCTATCGG
    AGAATCATGT CTGTGAAAAG ACCGGATGTC CTGAAAGCTA GACTGTGGAT CGAATTTGAA
    TCAGAGAAAG GTCTTGACTA TGGGGGTGTG GCCAGAGAGT GGTTCTTCCT CCTGTCCAAA
    GAGATGTTCA ACCCCTACTA CGGGCTTTTT GAGTACTCTG CAACGGACAA CTACACACTT
    CAGATCAATC CCAATTCAGG CCTCTGTAAT GAAGACCATT TGTCCTATTT CACTTTTATT
    GGAAGAGTTG CTGGTCTGGC AGTATTTCAT GGGAAACTTC TAGATGGTTT CTTCATTAGA
    CCGTTTTACA AAATGATGTT GGGAAAGCAG ATAACTCTGA ATGACATGGA ATCTGTGGAT
    AGTGAATATT ACAACTCTTT GAAATGGATC TTGGAAAATG ATCCTACTGA ACTGGACCTC
    ATGTTCTGTA TCGATGAAGA AAACTTTGGG CAGACCTATC AAGTGGATCT GAAGCCCAAT
    GGGTCAGAAA TAATGGTGAC AAATGAAAAC AAGAGAGAAT ATATTGACTT AGTCATCCAG
    TGGAGGTTTG TGAACAGGGT CCAGAAGCAG ATGAACGCCT TCTTGGAGGG ATTCACAGAA
    CTGCTTCCTA TTGATTTGAT TAAAATTTTC GATGAAAATG AGCTGGAGTT GCTGATGTGC
    GGCCTTGGCG ATGTGGACGT GAACGATTGG AGACAGCATT CTATTTACAA GAATGGCTAC
    TGCCCCAACC ACCCTGTCAT TCAGTGGTTC TGGAAGGCTG TGCTGCTCAT GGACGCGGAG
    AAGCGGATCC GGCTACTGCA GTTCGTCACG GGGACGTCCC GGGTACCTAT GAACGGATTC
    GCCGAACTTT ATGGTTCCAA TGGTCCTCAG CTGTTTACAA TAGAGCAATG GGGGAGTCCT
    GACAAGCTGC CCAGAGCTCA CACGTGCTTT AATCGCCTTG ACTTACCTCC ATATGAAACC
    TTTGAAGATT TACGAGAGAA ACTCCTCATG GCTGTGGAAA ACGCTCAAGG ATTTGAGGGG
    GTGGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe00764
    Clone ID Related Accession (Same CDS sequence) XM_005615369.2 , XM_005615369.3
    Accession Version XM_005615369.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2829bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005615369.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAGCCTTG AGGACAATTA CAGTGATCCG TATGTGAAAC TTTCATTGTA CGTAGCAGAT 
    GAGAATAGAG AACTCGCTTT GGTACAGACA AAAACCATTA AAAAGACGCT GAACCCAAAG
    TGGAATGAAG AATTTTATTT TAGAGTAAAC CCATCTAATC ACAGACTCCT GTTTGAAGTA
    TTTGATGAAA ACAGATTGAC ACGAGACGAC TTCCTGGGAC AGGTGGACAT ACCCCTTAGT
    CACCTCCCGA CTGAAGATCC GACCATGGAG CGACCCTATA CATTTAAGGA CTTTCTCCTT
    CGACCAAGAA GTCATAAATC TCGAGTTAAG GGATTTTTGC GATTGAAAAT GGCCTATATG
    CCAAAAAATG GCGGTCAAGA TGAAGAAAAC AGTGAACAGA GGGATGATAT GGAGCATGGC
    TGGGAGGTTG TGGACTCGAA TGACTCGGCC TCTCAGCACC AGGAGGAGCT TCCTCCACCC
    CCACTGCCCC CAGGCTGGGA AGAGAAGGTG GACAACCTTG GTCGTACCTA TTATGTCAAC
    CACAACAACC GGACCACCCA GTGGCACCGA CCAAGCTTGA TGGATGTGTC CTCCGAGTCC
    GACAGCAACA TCAGGCAGAT CAACCAGGAG GCCGCGCACC GGCGCTTCCG CTCACGCAGG
    CACATCAGCG AGGACCTGGA GCCCGAGCCT GGCGAGGGTG GAGACGGCCC AGAGCCTTGG
    GAGACCATCT CCGAAGAAGT GAACATCACG GGAGACCCCC TCAGTCTGGC GCTGCCCCCA
    CCACCATCCT CTCCAGTATC TCGGACCAGC CCTCAGGAGC TGTCTGAGGA ACTGAGCAGA
    AGGCTTCAGA TCACTCCAGA CTCAAATGGG GAGCAATTTG GTTCTTTGAT GCAAAGAGAG
    CCCTCCTCAA GGCTGCGGTC CTGCAGTGTC ACCGACGGGG TCGCCGAGCA GGCTCATCTA
    CCACCGCCGA GTGTCCCAAC TAGGAGAGCG CGGTCATCCA CCGTCACGGG TGGTGAGGAG
    CCAACGCCAT CAGTGGCCTA TGCACACACC ACGCCGGGCC TACCTTCAGG CTGGGAAGAA
    AGAAAAGATG CTAAGGGACG CACATACTAT GTCAATCATA ACAATCGAAC CACAACTTGG
    ACTCGACCTA TCATGCAGCT CGCCGAAGAC GGTGCGTCCG GATCCGCCAC AAACAGTAAC
    AACCACCTAA TCGAGCCTCA GATCCGCCGG CCCCGTAGCC TCAGCTCGCC GACCGTAACC
    TTATCTGCCC CACTGGAGGG TGCCAAGGAT TCGCCCATAC GTCGGGCTGT GAAAGATACC
    CTTTCCAATC CACAGTCCCC ACAGCCATCA CCTTACAACT CCCCCAAACC ACAACACAAA
    GTCACACAGA GCTTCCTGCC GCCCGGCTGG GAAATGAGGA TAGCGCCCAA CGGCCGGCCC
    TTCTTCATTG ACCATAACAC AAAGACTACA ACGTGGGAAG ATCCACGCCT GAAATTTCCA
    GTACATATGC GGTCAAAGGC ATCCTTAAAC CCCAATGACC TTGGCCCTCT TCCTCCTGGT
    TGGGAAGAAA GAATTCACTT GGATGGCCGA ACGTTTTATA TTGACCATAA TAGCAAAATT
    ACTCAGTGGG AAGATCCAAG ACTGCAGAAT CCAGCTATTA CTGGTCCGGC TGTTCCTTAC
    TCCAGAGAAT TTAAGCAGAA ATATGACTAC TTTAGGAAGA AATTAAAGAA ACCTGCTGAT
    ATTCCAAATA GGTTTGAAAT GAAACTTCAC AGAAATAACA TCTTTGAAGA GTCCTATCGG
    AGAATCATGT CTGTGAAAAG ACCGGATGTC CTGAAAGCTA GACTGTGGAT CGAATTTGAA
    TCAGAGAAAG GTCTTGACTA TGGGGGTGTG GCCAGAGAGT GGTTCTTCCT CCTGTCCAAA
    GAGATGTTCA ACCCCTACTA CGGGCTTTTT GAGTACTCTG CAACGGACAA CTACACACTT
    CAGATCAATC CCAATTCAGG CCTCTGTAAT GAAGACCATT TGTCCTATTT CACTTTTATT
    GGAAGAGTTG CTGGTCTGGC AGTATTTCAT GGGAAACTTC TAGATGGTTT CTTCATTAGA
    CCGTTTTACA AAATGATGTT GGGAAAGCAG ATAACTCTGA ATGACATGGA ATCTGTGGAT
    AGTGAATATT ACAACTCTTT GAAATGGATC TTGGAAAATG ATCCTACTGA ACTGGACCTC
    ATGTTCTGTA TCGATGAAGA AAACTTTGGG CAGACCTATC AAGTGGATCT GAAGCCCAAT
    GGGTCAGAAA TAATGGTGAC AAATGAAAAC AAGAGAGAAT ATATTGACTT AGTCATCCAG
    TGGAGGTTTG TGAACAGGGT CCAGAAGCAG ATGAACGCCT TCTTGGAGGG ATTCACAGAA
    CTGCTTCCTA TTGATTTGAT TAAAATTTTC GATGAAAATG AGCTGGAGTT GCTGATGTGC
    GGCCTTGGCG ATGTGGACGT GAACGATTGG AGACAGCATT CTATTTACAA GAATGGCTAC
    TGCCCCAACC ACCCTGTCAT TCAGTGGTTC TGGAAGGCTG TGCTGCTCAT GGACGCGGAG
    AAGCGGATCC GGCTACTGCA GTTCGTCACG GGGACGTCCC GGGTACCTAT GAACGGATTC
    GCCGAACTTT ATGGTTCCAA TGGTCCTCAG CTGTTTACAA TAGAGCAATG GGGGAGTCCT
    GACAAGCTGC CCAGAGCTCA CACGTGCTTT AATCGCCTTG ACTTACCTCC ATATGAAACC
    TTTGAAGATT TACGAGAGAA ACTCCTCATG GCTGTGGAAA ACGCTCAAGG ATTTGAGGGG
    GTGGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005615426.1
    CDS207..3035
    Translation

    Target ORF information:

    RefSeq Version XM_005615369.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005615369.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAGCCTTG AGGACAATTA CAGTGATCCG TATGTGAAAC TTTCATTGTA CGTAGCAGAT 
    GAGAATAGAG AACTCGCTTT GGTACAGACA AAAACCATTA AAAAGACGCT GAACCCAAAG
    TGGAATGAAG AATTTTATTT TAGAGTAAAC CCATCTAATC ACAGACTCCT GTTTGAAGTA
    TTTGATGAAA ACAGATTGAC ACGAGACGAC TTCCTGGGAC AGGTGGACAT ACCCCTTAGT
    CACCTCCCGA CTGAAGATCC GACCATGGAG CGACCCTATA CATTTAAGGA CTTTCTCCTT
    CGACCAAGAA GTCATAAATC TCGAGTTAAG GGATTTTTGC GATTGAAAAT GGCCTATATG
    CCAAAAAATG GCGGTCAAGA TGAAGAAAAC AGTGAACAGA GGGATGATAT GGAGCATGGC
    TGGGAGGTTG TGGACTCGAA TGACTCGGCC TCTCAGCACC AGGAGGAGCT TCCTCCACCC
    CCACTGCCCC CAGGCTGGGA AGAGAAGGTG GACAACCTTG GTCGTACCTA TTATGTCAAC
    CACAACAACC GGACCACCCA GTGGCACCGA CCAAGCTTGA TGGATGTGTC CTCCGAGTCC
    GACAGCAACA TCAGGCAGAT CAACCAGGAG GCCGCGCACC GGCGCTTCCG CTCACGCAGG
    CACATCAGCG AGGACCTGGA GCCCGAGCCT GGCGAGGGTG GAGACGGCCC AGAGCCTTGG
    GAGACCATCT CCGAAGAAGT GAACATCACG GGAGACCCCC TCAGTCTGGC GCTGCCCCCA
    CCACCATCCT CTCCAGTATC TCGGACCAGC CCTCAGGAGC TGTCTGAGGA ACTGAGCAGA
    AGGCTTCAGA TCACTCCAGA CTCAAATGGG GAGCAATTTG GTTCTTTGAT GCAAAGAGAG
    CCCTCCTCAA GGCTGCGGTC CTGCAGTGTC ACCGACGGGG TCGCCGAGCA GGCTCATCTA
    CCACCGCCGA GTGTCCCAAC TAGGAGAGCG CGGTCATCCA CCGTCACGGG TGGTGAGGAG
    CCAACGCCAT CAGTGGCCTA TGCACACACC ACGCCGGGCC TACCTTCAGG CTGGGAAGAA
    AGAAAAGATG CTAAGGGACG CACATACTAT GTCAATCATA ACAATCGAAC CACAACTTGG
    ACTCGACCTA TCATGCAGCT CGCCGAAGAC GGTGCGTCCG GATCCGCCAC AAACAGTAAC
    AACCACCTAA TCGAGCCTCA GATCCGCCGG CCCCGTAGCC TCAGCTCGCC GACCGTAACC
    TTATCTGCCC CACTGGAGGG TGCCAAGGAT TCGCCCATAC GTCGGGCTGT GAAAGATACC
    CTTTCCAATC CACAGTCCCC ACAGCCATCA CCTTACAACT CCCCCAAACC ACAACACAAA
    GTCACACAGA GCTTCCTGCC GCCCGGCTGG GAAATGAGGA TAGCGCCCAA CGGCCGGCCC
    TTCTTCATTG ACCATAACAC AAAGACTACA ACGTGGGAAG ATCCACGCCT GAAATTTCCA
    GTACATATGC GGTCAAAGGC ATCCTTAAAC CCCAATGACC TTGGCCCTCT TCCTCCTGGT
    TGGGAAGAAA GAATTCACTT GGATGGCCGA ACGTTTTATA TTGACCATAA TAGCAAAATT
    ACTCAGTGGG AAGATCCAAG ACTGCAGAAT CCAGCTATTA CTGGTCCGGC TGTTCCTTAC
    TCCAGAGAAT TTAAGCAGAA ATATGACTAC TTTAGGAAGA AATTAAAGAA ACCTGCTGAT
    ATTCCAAATA GGTTTGAAAT GAAACTTCAC AGAAATAACA TCTTTGAAGA GTCCTATCGG
    AGAATCATGT CTGTGAAAAG ACCGGATGTC CTGAAAGCTA GACTGTGGAT CGAATTTGAA
    TCAGAGAAAG GTCTTGACTA TGGGGGTGTG GCCAGAGAGT GGTTCTTCCT CCTGTCCAAA
    GAGATGTTCA ACCCCTACTA CGGGCTTTTT GAGTACTCTG CAACGGACAA CTACACACTT
    CAGATCAATC CCAATTCAGG CCTCTGTAAT GAAGACCATT TGTCCTATTT CACTTTTATT
    GGAAGAGTTG CTGGTCTGGC AGTATTTCAT GGGAAACTTC TAGATGGTTT CTTCATTAGA
    CCGTTTTACA AAATGATGTT GGGAAAGCAG ATAACTCTGA ATGACATGGA ATCTGTGGAT
    AGTGAATATT ACAACTCTTT GAAATGGATC TTGGAAAATG ATCCTACTGA ACTGGACCTC
    ATGTTCTGTA TCGATGAAGA AAACTTTGGG CAGACCTATC AAGTGGATCT GAAGCCCAAT
    GGGTCAGAAA TAATGGTGAC AAATGAAAAC AAGAGAGAAT ATATTGACTT AGTCATCCAG
    TGGAGGTTTG TGAACAGGGT CCAGAAGCAG ATGAACGCCT TCTTGGAGGG ATTCACAGAA
    CTGCTTCCTA TTGATTTGAT TAAAATTTTC GATGAAAATG AGCTGGAGTT GCTGATGTGC
    GGCCTTGGCG ATGTGGACGT GAACGATTGG AGACAGCATT CTATTTACAA GAATGGCTAC
    TGCCCCAACC ACCCTGTCAT TCAGTGGTTC TGGAAGGCTG TGCTGCTCAT GGACGCGGAG
    AAGCGGATCC GGCTACTGCA GTTCGTCACG GGGACGTCCC GGGTACCTAT GAACGGATTC
    GCCGAACTTT ATGGTTCCAA TGGTCCTCAG CTGTTTACAA TAGAGCAATG GGGGAGTCCT
    GACAAGCTGC CCAGAGCTCA CACGTGCTTT AATCGCCTTG ACTTACCTCC ATATGAAACC
    TTTGAAGATT TACGAGAGAA ACTCCTCATG GCTGTGGAAA ACGCTCAAGG ATTTGAGGGG
    GTGGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe00766
    Clone ID Related Accession (Same CDS sequence) XM_005615370.2 , XM_005615370.3
    Accession Version XM_005615370.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2769bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726046.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005615370.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAGCCTTG AGGACAATTA CAGTGATCCG TATGTGAAAC TTTCATTGTA CGTAGCAGAT 
    GAGAATAGAG AACTCGCTTT GGTACAGACA AAAACCATTA AAAAGACGCT GAACCCAAAG
    TGGAATGAAG AATTTTATTT TAGAGTAAAC CCATCTAATC ACAGACTCCT GTTTGAAGTA
    TTTGATGAAA ACAGATTGAC ACGAGACGAC TTCCTGGGAC AGGTGGACAT ACCCCTTAGT
    CACCTCCCGA CTGAAGATCC GACCATGGAG CGACCCTATA CATTTAAGGA CTTTCTCCTT
    CGACCAAGAA GTCATAAATC TCGAGTTAAG GGATTTTTGC GATTGAAAAT GGCCTATATG
    CCAAAAAATG GCGGTCAAGA TGAAGAAAAC AGTGAACAGA GGGATGATAT GGAGCATGGC
    TGGGAGGTTG TGGACTCGAA TGACTCGGCC TCTCAGCACC AGGAGGAGCT TCCTCCACCC
    CCACTGCCCC CAGGCTGGGA AGAGAAGGTG GACAACCTTG GTCGTACCTA TTATGTCAAC
    CACAACAACC GGACCACCCA GTGGCACCGA CCAAGCTTGA TGGATGTGTC CTCCGAGTCC
    GACAGCAACA TCAGGCAGAT CAACCAGGAG GCCGCGCACC GGCGCTTCCG CTCACGCAGG
    CACATCAGCG AGGACCTGGA GCCCGAGCCT GGCGAGGGTG GAGACGGCCC AGAGCCTTGG
    GAGACCATCT CCGAAGAAGT GAACATCACG GGAGACCCCC TCAGTCTGGC GCTGCCCCCA
    CCACCATCCT CTCCAGTATC TCGGACCAGC CCTCAGGAGC TGTCTGAGGA ACTGAGCAGA
    AGGCTTCAGA TCACTCCAGA CTCAAATGGG GAGCAATTTG GTTCTTTGAT GCAAAGAGAG
    CCCTCCTCAA GGCTGCGGTC CTGCAGTGTC ACCGACGGGG TCGCCGAGCA GGCTCATCTA
    CCACCGCCAT CAGTGGCCTA TGCACACACC ACGCCGGGCC TACCTTCAGG CTGGGAAGAA
    AGAAAAGATG CTAAGGGACG CACATACTAT GTCAATCATA ACAATCGAAC CACAACTTGG
    ACTCGACCTA TCATGCAGCT CGCCGAAGAC GGTGCGTCCG GATCCGCCAC AAACAGTAAC
    AACCACCTAA TCGAGCCTCA GATCCGCCGG CCCCGTAGCC TCAGCTCGCC GACCGTAACC
    TTATCTGCCC CACTGGAGGG TGCCAAGGAT TCGCCCATAC GTCGGGCTGT GAAAGATACC
    CTTTCCAATC CACAGTCCCC ACAGCCATCA CCTTACAACT CCCCCAAACC ACAACACAAA
    GTCACACAGA GCTTCCTGCC GCCCGGCTGG GAAATGAGGA TAGCGCCCAA CGGCCGGCCC
    TTCTTCATTG ACCATAACAC AAAGACTACA ACGTGGGAAG ATCCACGCCT GAAATTTCCA
    GTACATATGC GGTCAAAGGC ATCCTTAAAC CCCAATGACC TTGGCCCTCT TCCTCCTGGT
    TGGGAAGAAA GAATTCACTT GGATGGCCGA ACGTTTTATA TTGACCATAA TAGCAAAATT
    ACTCAGTGGG AAGATCCAAG ACTGCAGAAT CCAGCTATTA CTGGTCCGGC TGTTCCTTAC
    TCCAGAGAAT TTAAGCAGAA ATATGACTAC TTTAGGAAGA AATTAAAGAA ACCTGCTGAT
    ATTCCAAATA GGTTTGAAAT GAAACTTCAC AGAAATAACA TCTTTGAAGA GTCCTATCGG
    AGAATCATGT CTGTGAAAAG ACCGGATGTC CTGAAAGCTA GACTGTGGAT CGAATTTGAA
    TCAGAGAAAG GTCTTGACTA TGGGGGTGTG GCCAGAGAGT GGTTCTTCCT CCTGTCCAAA
    GAGATGTTCA ACCCCTACTA CGGGCTTTTT GAGTACTCTG CAACGGACAA CTACACACTT
    CAGATCAATC CCAATTCAGG CCTCTGTAAT GAAGACCATT TGTCCTATTT CACTTTTATT
    GGAAGAGTTG CTGGTCTGGC AGTATTTCAT GGGAAACTTC TAGATGGTTT CTTCATTAGA
    CCGTTTTACA AAATGATGTT GGGAAAGCAG ATAACTCTGA ATGACATGGA ATCTGTGGAT
    AGTGAATATT ACAACTCTTT GAAATGGATC TTGGAAAATG ATCCTACTGA ACTGGACCTC
    ATGTTCTGTA TCGATGAAGA AAACTTTGGG CAGACCTATC AAGTGGATCT GAAGCCCAAT
    GGGTCAGAAA TAATGGTGAC AAATGAAAAC AAGAGAGAAT ATATTGACTT AGTCATCCAG
    TGGAGGTTTG TGAACAGGGT CCAGAAGCAG ATGAACGCCT TCTTGGAGGG ATTCACAGAA
    CTGCTTCCTA TTGATTTGAT TAAAATTTTC GATGAAAATG AGCTGGAGTT GCTGATGTGC
    GGCCTTGGCG ATGTGGACGT GAACGATTGG AGACAGCATT CTATTTACAA GAATGGCTAC
    TGCCCCAACC ACCCTGTCAT TCAGTGGTTC TGGAAGGCTG TGCTGCTCAT GGACGCGGAG
    AAGCGGATCC GGCTACTGCA GTTCGTCACG GGGACGTCCC GGGTACCTAT GAACGGATTC
    GCCGAACTTT ATGGTTCCAA TGGTCCTCAG CTGTTTACAA TAGAGCAATG GGGGAGTCCT
    GACAAGCTGC CCAGAGCTCA CACGTGCTTT AATCGCCTTG ACTTACCTCC ATATGAAACC
    TTTGAAGATT TACGAGAGAA ACTCCTCATG GCTGTGGAAA ACGCTCAAGG ATTTGAGGGG
    GTGGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005615427.1
    CDS222..2990
    Translation

    Target ORF information:

    RefSeq Version XM_005615370.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005615370.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAGCCTTG AGGACAATTA CAGTGATCCG TATGTGAAAC TTTCATTGTA CGTAGCAGAT 
    GAGAATAGAG AACTCGCTTT GGTACAGACA AAAACCATTA AAAAGACGCT GAACCCAAAG
    TGGAATGAAG AATTTTATTT TAGAGTAAAC CCATCTAATC ACAGACTCCT GTTTGAAGTA
    TTTGATGAAA ACAGATTGAC ACGAGACGAC TTCCTGGGAC AGGTGGACAT ACCCCTTAGT
    CACCTCCCGA CTGAAGATCC GACCATGGAG CGACCCTATA CATTTAAGGA CTTTCTCCTT
    CGACCAAGAA GTCATAAATC TCGAGTTAAG GGATTTTTGC GATTGAAAAT GGCCTATATG
    CCAAAAAATG GCGGTCAAGA TGAAGAAAAC AGTGAACAGA GGGATGATAT GGAGCATGGC
    TGGGAGGTTG TGGACTCGAA TGACTCGGCC TCTCAGCACC AGGAGGAGCT TCCTCCACCC
    CCACTGCCCC CAGGCTGGGA AGAGAAGGTG GACAACCTTG GTCGTACCTA TTATGTCAAC
    CACAACAACC GGACCACCCA GTGGCACCGA CCAAGCTTGA TGGATGTGTC CTCCGAGTCC
    GACAGCAACA TCAGGCAGAT CAACCAGGAG GCCGCGCACC GGCGCTTCCG CTCACGCAGG
    CACATCAGCG AGGACCTGGA GCCCGAGCCT GGCGAGGGTG GAGACGGCCC AGAGCCTTGG
    GAGACCATCT CCGAAGAAGT GAACATCACG GGAGACCCCC TCAGTCTGGC GCTGCCCCCA
    CCACCATCCT CTCCAGTATC TCGGACCAGC CCTCAGGAGC TGTCTGAGGA ACTGAGCAGA
    AGGCTTCAGA TCACTCCAGA CTCAAATGGG GAGCAATTTG GTTCTTTGAT GCAAAGAGAG
    CCCTCCTCAA GGCTGCGGTC CTGCAGTGTC ACCGACGGGG TCGCCGAGCA GGCTCATCTA
    CCACCGCCAT CAGTGGCCTA TGCACACACC ACGCCGGGCC TACCTTCAGG CTGGGAAGAA
    AGAAAAGATG CTAAGGGACG CACATACTAT GTCAATCATA ACAATCGAAC CACAACTTGG
    ACTCGACCTA TCATGCAGCT CGCCGAAGAC GGTGCGTCCG GATCCGCCAC AAACAGTAAC
    AACCACCTAA TCGAGCCTCA GATCCGCCGG CCCCGTAGCC TCAGCTCGCC GACCGTAACC
    TTATCTGCCC CACTGGAGGG TGCCAAGGAT TCGCCCATAC GTCGGGCTGT GAAAGATACC
    CTTTCCAATC CACAGTCCCC ACAGCCATCA CCTTACAACT CCCCCAAACC ACAACACAAA
    GTCACACAGA GCTTCCTGCC GCCCGGCTGG GAAATGAGGA TAGCGCCCAA CGGCCGGCCC
    TTCTTCATTG ACCATAACAC AAAGACTACA ACGTGGGAAG ATCCACGCCT GAAATTTCCA
    GTACATATGC GGTCAAAGGC ATCCTTAAAC CCCAATGACC TTGGCCCTCT TCCTCCTGGT
    TGGGAAGAAA GAATTCACTT GGATGGCCGA ACGTTTTATA TTGACCATAA TAGCAAAATT
    ACTCAGTGGG AAGATCCAAG ACTGCAGAAT CCAGCTATTA CTGGTCCGGC TGTTCCTTAC
    TCCAGAGAAT TTAAGCAGAA ATATGACTAC TTTAGGAAGA AATTAAAGAA ACCTGCTGAT
    ATTCCAAATA GGTTTGAAAT GAAACTTCAC AGAAATAACA TCTTTGAAGA GTCCTATCGG
    AGAATCATGT CTGTGAAAAG ACCGGATGTC CTGAAAGCTA GACTGTGGAT CGAATTTGAA
    TCAGAGAAAG GTCTTGACTA TGGGGGTGTG GCCAGAGAGT GGTTCTTCCT CCTGTCCAAA
    GAGATGTTCA ACCCCTACTA CGGGCTTTTT GAGTACTCTG CAACGGACAA CTACACACTT
    CAGATCAATC CCAATTCAGG CCTCTGTAAT GAAGACCATT TGTCCTATTT CACTTTTATT
    GGAAGAGTTG CTGGTCTGGC AGTATTTCAT GGGAAACTTC TAGATGGTTT CTTCATTAGA
    CCGTTTTACA AAATGATGTT GGGAAAGCAG ATAACTCTGA ATGACATGGA ATCTGTGGAT
    AGTGAATATT ACAACTCTTT GAAATGGATC TTGGAAAATG ATCCTACTGA ACTGGACCTC
    ATGTTCTGTA TCGATGAAGA AAACTTTGGG CAGACCTATC AAGTGGATCT GAAGCCCAAT
    GGGTCAGAAA TAATGGTGAC AAATGAAAAC AAGAGAGAAT ATATTGACTT AGTCATCCAG
    TGGAGGTTTG TGAACAGGGT CCAGAAGCAG ATGAACGCCT TCTTGGAGGG ATTCACAGAA
    CTGCTTCCTA TTGATTTGAT TAAAATTTTC GATGAAAATG AGCTGGAGTT GCTGATGTGC
    GGCCTTGGCG ATGTGGACGT GAACGATTGG AGACAGCATT CTATTTACAA GAATGGCTAC
    TGCCCCAACC ACCCTGTCAT TCAGTGGTTC TGGAAGGCTG TGCTGCTCAT GGACGCGGAG
    AAGCGGATCC GGCTACTGCA GTTCGTCACG GGGACGTCCC GGGTACCTAT GAACGGATTC
    GCCGAACTTT ATGGTTCCAA TGGTCCTCAG CTGTTTACAA TAGAGCAATG GGGGAGTCCT
    GACAAGCTGC CCAGAGCTCA CACGTGCTTT AATCGCCTTG ACTTACCTCC ATATGAAACC
    TTTGAAGATT TACGAGAGAA ACTCCTCATG GCTGTGGAAA ACGCTCAAGG ATTTGAGGGG
    GTGGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe00766
    Clone ID Related Accession (Same CDS sequence) XM_005615370.2 , XM_005615370.3
    Accession Version XM_005615370.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2769bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005615370.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAGCCTTG AGGACAATTA CAGTGATCCG TATGTGAAAC TTTCATTGTA CGTAGCAGAT 
    GAGAATAGAG AACTCGCTTT GGTACAGACA AAAACCATTA AAAAGACGCT GAACCCAAAG
    TGGAATGAAG AATTTTATTT TAGAGTAAAC CCATCTAATC ACAGACTCCT GTTTGAAGTA
    TTTGATGAAA ACAGATTGAC ACGAGACGAC TTCCTGGGAC AGGTGGACAT ACCCCTTAGT
    CACCTCCCGA CTGAAGATCC GACCATGGAG CGACCCTATA CATTTAAGGA CTTTCTCCTT
    CGACCAAGAA GTCATAAATC TCGAGTTAAG GGATTTTTGC GATTGAAAAT GGCCTATATG
    CCAAAAAATG GCGGTCAAGA TGAAGAAAAC AGTGAACAGA GGGATGATAT GGAGCATGGC
    TGGGAGGTTG TGGACTCGAA TGACTCGGCC TCTCAGCACC AGGAGGAGCT TCCTCCACCC
    CCACTGCCCC CAGGCTGGGA AGAGAAGGTG GACAACCTTG GTCGTACCTA TTATGTCAAC
    CACAACAACC GGACCACCCA GTGGCACCGA CCAAGCTTGA TGGATGTGTC CTCCGAGTCC
    GACAGCAACA TCAGGCAGAT CAACCAGGAG GCCGCGCACC GGCGCTTCCG CTCACGCAGG
    CACATCAGCG AGGACCTGGA GCCCGAGCCT GGCGAGGGTG GAGACGGCCC AGAGCCTTGG
    GAGACCATCT CCGAAGAAGT GAACATCACG GGAGACCCCC TCAGTCTGGC GCTGCCCCCA
    CCACCATCCT CTCCAGTATC TCGGACCAGC CCTCAGGAGC TGTCTGAGGA ACTGAGCAGA
    AGGCTTCAGA TCACTCCAGA CTCAAATGGG GAGCAATTTG GTTCTTTGAT GCAAAGAGAG
    CCCTCCTCAA GGCTGCGGTC CTGCAGTGTC ACCGACGGGG TCGCCGAGCA GGCTCATCTA
    CCACCGCCAT CAGTGGCCTA TGCACACACC ACGCCGGGCC TACCTTCAGG CTGGGAAGAA
    AGAAAAGATG CTAAGGGACG CACATACTAT GTCAATCATA ACAATCGAAC CACAACTTGG
    ACTCGACCTA TCATGCAGCT CGCCGAAGAC GGTGCGTCCG GATCCGCCAC AAACAGTAAC
    AACCACCTAA TCGAGCCTCA GATCCGCCGG CCCCGTAGCC TCAGCTCGCC GACCGTAACC
    TTATCTGCCC CACTGGAGGG TGCCAAGGAT TCGCCCATAC GTCGGGCTGT GAAAGATACC
    CTTTCCAATC CACAGTCCCC ACAGCCATCA CCTTACAACT CCCCCAAACC ACAACACAAA
    GTCACACAGA GCTTCCTGCC GCCCGGCTGG GAAATGAGGA TAGCGCCCAA CGGCCGGCCC
    TTCTTCATTG ACCATAACAC AAAGACTACA ACGTGGGAAG ATCCACGCCT GAAATTTCCA
    GTACATATGC GGTCAAAGGC ATCCTTAAAC CCCAATGACC TTGGCCCTCT TCCTCCTGGT
    TGGGAAGAAA GAATTCACTT GGATGGCCGA ACGTTTTATA TTGACCATAA TAGCAAAATT
    ACTCAGTGGG AAGATCCAAG ACTGCAGAAT CCAGCTATTA CTGGTCCGGC TGTTCCTTAC
    TCCAGAGAAT TTAAGCAGAA ATATGACTAC TTTAGGAAGA AATTAAAGAA ACCTGCTGAT
    ATTCCAAATA GGTTTGAAAT GAAACTTCAC AGAAATAACA TCTTTGAAGA GTCCTATCGG
    AGAATCATGT CTGTGAAAAG ACCGGATGTC CTGAAAGCTA GACTGTGGAT CGAATTTGAA
    TCAGAGAAAG GTCTTGACTA TGGGGGTGTG GCCAGAGAGT GGTTCTTCCT CCTGTCCAAA
    GAGATGTTCA ACCCCTACTA CGGGCTTTTT GAGTACTCTG CAACGGACAA CTACACACTT
    CAGATCAATC CCAATTCAGG CCTCTGTAAT GAAGACCATT TGTCCTATTT CACTTTTATT
    GGAAGAGTTG CTGGTCTGGC AGTATTTCAT GGGAAACTTC TAGATGGTTT CTTCATTAGA
    CCGTTTTACA AAATGATGTT GGGAAAGCAG ATAACTCTGA ATGACATGGA ATCTGTGGAT
    AGTGAATATT ACAACTCTTT GAAATGGATC TTGGAAAATG ATCCTACTGA ACTGGACCTC
    ATGTTCTGTA TCGATGAAGA AAACTTTGGG CAGACCTATC AAGTGGATCT GAAGCCCAAT
    GGGTCAGAAA TAATGGTGAC AAATGAAAAC AAGAGAGAAT ATATTGACTT AGTCATCCAG
    TGGAGGTTTG TGAACAGGGT CCAGAAGCAG ATGAACGCCT TCTTGGAGGG ATTCACAGAA
    CTGCTTCCTA TTGATTTGAT TAAAATTTTC GATGAAAATG AGCTGGAGTT GCTGATGTGC
    GGCCTTGGCG ATGTGGACGT GAACGATTGG AGACAGCATT CTATTTACAA GAATGGCTAC
    TGCCCCAACC ACCCTGTCAT TCAGTGGTTC TGGAAGGCTG TGCTGCTCAT GGACGCGGAG
    AAGCGGATCC GGCTACTGCA GTTCGTCACG GGGACGTCCC GGGTACCTAT GAACGGATTC
    GCCGAACTTT ATGGTTCCAA TGGTCCTCAG CTGTTTACAA TAGAGCAATG GGGGAGTCCT
    GACAAGCTGC CCAGAGCTCA CACGTGCTTT AATCGCCTTG ACTTACCTCC ATATGAAACC
    TTTGAAGATT TACGAGAGAA ACTCCTCATG GCTGTGGAAA ACGCTCAAGG ATTTGAGGGG
    GTGGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005615427.1
    CDS205..2973
    Translation

    Target ORF information:

    RefSeq Version XM_005615370.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005615370.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAGCCTTG AGGACAATTA CAGTGATCCG TATGTGAAAC TTTCATTGTA CGTAGCAGAT 
    GAGAATAGAG AACTCGCTTT GGTACAGACA AAAACCATTA AAAAGACGCT GAACCCAAAG
    TGGAATGAAG AATTTTATTT TAGAGTAAAC CCATCTAATC ACAGACTCCT GTTTGAAGTA
    TTTGATGAAA ACAGATTGAC ACGAGACGAC TTCCTGGGAC AGGTGGACAT ACCCCTTAGT
    CACCTCCCGA CTGAAGATCC GACCATGGAG CGACCCTATA CATTTAAGGA CTTTCTCCTT
    CGACCAAGAA GTCATAAATC TCGAGTTAAG GGATTTTTGC GATTGAAAAT GGCCTATATG
    CCAAAAAATG GCGGTCAAGA TGAAGAAAAC AGTGAACAGA GGGATGATAT GGAGCATGGC
    TGGGAGGTTG TGGACTCGAA TGACTCGGCC TCTCAGCACC AGGAGGAGCT TCCTCCACCC
    CCACTGCCCC CAGGCTGGGA AGAGAAGGTG GACAACCTTG GTCGTACCTA TTATGTCAAC
    CACAACAACC GGACCACCCA GTGGCACCGA CCAAGCTTGA TGGATGTGTC CTCCGAGTCC
    GACAGCAACA TCAGGCAGAT CAACCAGGAG GCCGCGCACC GGCGCTTCCG CTCACGCAGG
    CACATCAGCG AGGACCTGGA GCCCGAGCCT GGCGAGGGTG GAGACGGCCC AGAGCCTTGG
    GAGACCATCT CCGAAGAAGT GAACATCACG GGAGACCCCC TCAGTCTGGC GCTGCCCCCA
    CCACCATCCT CTCCAGTATC TCGGACCAGC CCTCAGGAGC TGTCTGAGGA ACTGAGCAGA
    AGGCTTCAGA TCACTCCAGA CTCAAATGGG GAGCAATTTG GTTCTTTGAT GCAAAGAGAG
    CCCTCCTCAA GGCTGCGGTC CTGCAGTGTC ACCGACGGGG TCGCCGAGCA GGCTCATCTA
    CCACCGCCAT CAGTGGCCTA TGCACACACC ACGCCGGGCC TACCTTCAGG CTGGGAAGAA
    AGAAAAGATG CTAAGGGACG CACATACTAT GTCAATCATA ACAATCGAAC CACAACTTGG
    ACTCGACCTA TCATGCAGCT CGCCGAAGAC GGTGCGTCCG GATCCGCCAC AAACAGTAAC
    AACCACCTAA TCGAGCCTCA GATCCGCCGG CCCCGTAGCC TCAGCTCGCC GACCGTAACC
    TTATCTGCCC CACTGGAGGG TGCCAAGGAT TCGCCCATAC GTCGGGCTGT GAAAGATACC
    CTTTCCAATC CACAGTCCCC ACAGCCATCA CCTTACAACT CCCCCAAACC ACAACACAAA
    GTCACACAGA GCTTCCTGCC GCCCGGCTGG GAAATGAGGA TAGCGCCCAA CGGCCGGCCC
    TTCTTCATTG ACCATAACAC AAAGACTACA ACGTGGGAAG ATCCACGCCT GAAATTTCCA
    GTACATATGC GGTCAAAGGC ATCCTTAAAC CCCAATGACC TTGGCCCTCT TCCTCCTGGT
    TGGGAAGAAA GAATTCACTT GGATGGCCGA ACGTTTTATA TTGACCATAA TAGCAAAATT
    ACTCAGTGGG AAGATCCAAG ACTGCAGAAT CCAGCTATTA CTGGTCCGGC TGTTCCTTAC
    TCCAGAGAAT TTAAGCAGAA ATATGACTAC TTTAGGAAGA AATTAAAGAA ACCTGCTGAT
    ATTCCAAATA GGTTTGAAAT GAAACTTCAC AGAAATAACA TCTTTGAAGA GTCCTATCGG
    AGAATCATGT CTGTGAAAAG ACCGGATGTC CTGAAAGCTA GACTGTGGAT CGAATTTGAA
    TCAGAGAAAG GTCTTGACTA TGGGGGTGTG GCCAGAGAGT GGTTCTTCCT CCTGTCCAAA
    GAGATGTTCA ACCCCTACTA CGGGCTTTTT GAGTACTCTG CAACGGACAA CTACACACTT
    CAGATCAATC CCAATTCAGG CCTCTGTAAT GAAGACCATT TGTCCTATTT CACTTTTATT
    GGAAGAGTTG CTGGTCTGGC AGTATTTCAT GGGAAACTTC TAGATGGTTT CTTCATTAGA
    CCGTTTTACA AAATGATGTT GGGAAAGCAG ATAACTCTGA ATGACATGGA ATCTGTGGAT
    AGTGAATATT ACAACTCTTT GAAATGGATC TTGGAAAATG ATCCTACTGA ACTGGACCTC
    ATGTTCTGTA TCGATGAAGA AAACTTTGGG CAGACCTATC AAGTGGATCT GAAGCCCAAT
    GGGTCAGAAA TAATGGTGAC AAATGAAAAC AAGAGAGAAT ATATTGACTT AGTCATCCAG
    TGGAGGTTTG TGAACAGGGT CCAGAAGCAG ATGAACGCCT TCTTGGAGGG ATTCACAGAA
    CTGCTTCCTA TTGATTTGAT TAAAATTTTC GATGAAAATG AGCTGGAGTT GCTGATGTGC
    GGCCTTGGCG ATGTGGACGT GAACGATTGG AGACAGCATT CTATTTACAA GAATGGCTAC
    TGCCCCAACC ACCCTGTCAT TCAGTGGTTC TGGAAGGCTG TGCTGCTCAT GGACGCGGAG
    AAGCGGATCC GGCTACTGCA GTTCGTCACG GGGACGTCCC GGGTACCTAT GAACGGATTC
    GCCGAACTTT ATGGTTCCAA TGGTCCTCAG CTGTTTACAA TAGAGCAATG GGGGAGTCCT
    GACAAGCTGC CCAGAGCTCA CACGTGCTTT AATCGCCTTG ACTTACCTCC ATATGAAACC
    TTTGAAGATT TACGAGAGAA ACTCCTCATG GCTGTGGAAA ACGCTCAAGG ATTTGAGGGG
    GTGGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe00768
    Clone ID Related Accession (Same CDS sequence) XM_533393.5
    Accession Version XM_533393.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2565bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_533393.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAGCGAC CCTATACATT TAAGGACTTT CTCCTTCGAC CAAGAAGTCA TAAATCTCGA 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCAA AAAATGGCGG TCAAGATGAA
    GAAAACAGTG AACAGAGGGA TGATATGGAG CATGGCTGGG AGGTTGTGGA CTCGAATGAC
    TCGGCCTCTC AGCACCAGGA GGAGCTTCCT CCACCCCCAC TGCCCCCAGG CTGGGAAGAG
    AAGGTGGACA ACCTTGGTCG TACCTATTAT GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGACCAA GCTTGATGGA TGTGTCCTCC GAGTCCGACA GCAACATCAG GCAGATCAAC
    CAGGAGGCCG CGCACCGGCG CTTCCGCTCA CGCAGGCACA TCAGCGAGGA CCTGGAGCCC
    GAGCCTGGCG AGGGTGGAGA CGGCCCAGAG CCTTGGGAGA CCATCTCCGA AGAAGTGAAC
    ATCACGGGAG ACCCCCTCAG TCTGGCGCTG CCCCCACCAC CATCCTCTCC AGTATCTCGG
    ACCAGCCCTC AGGAGCTGTC TGAGGAACTG AGCAGAAGGC TTCAGATCAC TCCAGACTCA
    AATGGGGAGC AATTTGGTTC TTTGATGCAA AGAGAGCCCT CCTCAAGGCT GCGGTCCTGC
    AGTGTCACCG ACGGGGTCGC CGAGCAGGCT CATCTACCAC CGCCGAGTGT CCCAACTAGG
    AGAGCGCGGT CATCCACCGT CACGGGTGGT GAGGAGCCAA CGCCATCAGT GGCCTATGCA
    CACACCACGC CGGGCCTACC TTCAGGCTGG GAAGAAAGAA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GACCTATCAT GCAGCTCGCC
    GAAGACGGTG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCCC GTAGCCTCAG CTCGCCGACC GTAACCTTAT CTGCCCCACT GGAGGGTGCC
    AAGGATTCGC CCATACGTCG GGCTGTGAAA GATACCCTTT CCAATCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAAA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA TAACACAAAG
    ACTACAACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ATATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGTTGGG AAGAAAGAAT TCACTTGGAT
    GGCCGAACGT TTTATATTGA CCATAATAGC AAAATTACTC AGTGGGAAGA TCCAAGACTG
    CAGAATCCAG CTATTACTGG TCCGGCTGTT CCTTACTCCA GAGAATTTAA GCAGAAATAT
    GACTACTTTA GGAAGAAATT AAAGAAACCT GCTGATATTC CAAATAGGTT TGAAATGAAA
    CTTCACAGAA ATAACATCTT TGAAGAGTCC TATCGGAGAA TCATGTCTGT GAAAAGACCG
    GATGTCCTGA AAGCTAGACT GTGGATCGAA TTTGAATCAG AGAAAGGTCT TGACTATGGG
    GGTGTGGCCA GAGAGTGGTT CTTCCTCCTG TCCAAAGAGA TGTTCAACCC CTACTACGGG
    CTTTTTGAGT ACTCTGCAAC GGACAACTAC ACACTTCAGA TCAATCCCAA TTCAGGCCTC
    TGTAATGAAG ACCATTTGTC CTATTTCACT TTTATTGGAA GAGTTGCTGG TCTGGCAGTA
    TTTCATGGGA AACTTCTAGA TGGTTTCTTC ATTAGACCGT TTTACAAAAT GATGTTGGGA
    AAGCAGATAA CTCTGAATGA CATGGAATCT GTGGATAGTG AATATTACAA CTCTTTGAAA
    TGGATCTTGG AAAATGATCC TACTGAACTG GACCTCATGT TCTGTATCGA TGAAGAAAAC
    TTTGGGCAGA CCTATCAAGT GGATCTGAAG CCCAATGGGT CAGAAATAAT GGTGACAAAT
    GAAAACAAGA GAGAATATAT TGACTTAGTC ATCCAGTGGA GGTTTGTGAA CAGGGTCCAG
    AAGCAGATGA ACGCCTTCTT GGAGGGATTC ACAGAACTGC TTCCTATTGA TTTGATTAAA
    ATTTTCGATG AAAATGAGCT GGAGTTGCTG ATGTGCGGCC TTGGCGATGT GGACGTGAAC
    GATTGGAGAC AGCATTCTAT TTACAAGAAT GGCTACTGCC CCAACCACCC TGTCATTCAG
    TGGTTCTGGA AGGCTGTGCT GCTCATGGAC GCGGAGAAGC GGATCCGGCT ACTGCAGTTC
    GTCACGGGGA CGTCCCGGGT ACCTATGAAC GGATTCGCCG AACTTTATGG TTCCAATGGT
    CCTCAGCTGT TTACAATAGA GCAATGGGGG AGTCCTGACA AGCTGCCCAG AGCTCACACG
    TGCTTTAATC GCCTTGACTT ACCTCCATAT GAAACCTTTG AAGATTTACG AGAGAAACTC
    CTCATGGCTG TGGAAAACGC TCAAGGATTT GAGGGGGTGG ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_533393.3
    CDS316..2880
    Translation

    Target ORF information:

    RefSeq Version XM_533393.5
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_533393.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAGCGAC CCTATACATT TAAGGACTTT CTCCTTCGAC CAAGAAGTCA TAAATCTCGA 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCAA AAAATGGCGG TCAAGATGAA
    GAAAACAGTG AACAGAGGGA TGATATGGAG CATGGCTGGG AGGTTGTGGA CTCGAATGAC
    TCGGCCTCTC AGCACCAGGA GGAGCTTCCT CCACCCCCAC TGCCCCCAGG CTGGGAAGAG
    AAGGTGGACA ACCTTGGTCG TACCTATTAT GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGACCAA GCTTGATGGA TGTGTCCTCC GAGTCCGACA GCAACATCAG GCAGATCAAC
    CAGGAGGCCG CGCACCGGCG CTTCCGCTCA CGCAGGCACA TCAGCGAGGA CCTGGAGCCC
    GAGCCTGGCG AGGGTGGAGA CGGCCCAGAG CCTTGGGAGA CCATCTCCGA AGAAGTGAAC
    ATCACGGGAG ACCCCCTCAG TCTGGCGCTG CCCCCACCAC CATCCTCTCC AGTATCTCGG
    ACCAGCCCTC AGGAGCTGTC TGAGGAACTG AGCAGAAGGC TTCAGATCAC TCCAGACTCA
    AATGGGGAGC AATTTGGTTC TTTGATGCAA AGAGAGCCCT CCTCAAGGCT GCGGTCCTGC
    AGTGTCACCG ACGGGGTCGC CGAGCAGGCT CATCTACCAC CGCCGAGTGT CCCAACTAGG
    AGAGCGCGGT CATCCACCGT CACGGGTGGT GAGGAGCCAA CGCCATCAGT GGCCTATGCA
    CACACCACGC CGGGCCTACC TTCAGGCTGG GAAGAAAGAA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GACCTATCAT GCAGCTCGCC
    GAAGACGGTG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCCC GTAGCCTCAG CTCGCCGACC GTAACCTTAT CTGCCCCACT GGAGGGTGCC
    AAGGATTCGC CCATACGTCG GGCTGTGAAA GATACCCTTT CCAATCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAAA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA TAACACAAAG
    ACTACAACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ATATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGTTGGG AAGAAAGAAT TCACTTGGAT
    GGCCGAACGT TTTATATTGA CCATAATAGC AAAATTACTC AGTGGGAAGA TCCAAGACTG
    CAGAATCCAG CTATTACTGG TCCGGCTGTT CCTTACTCCA GAGAATTTAA GCAGAAATAT
    GACTACTTTA GGAAGAAATT AAAGAAACCT GCTGATATTC CAAATAGGTT TGAAATGAAA
    CTTCACAGAA ATAACATCTT TGAAGAGTCC TATCGGAGAA TCATGTCTGT GAAAAGACCG
    GATGTCCTGA AAGCTAGACT GTGGATCGAA TTTGAATCAG AGAAAGGTCT TGACTATGGG
    GGTGTGGCCA GAGAGTGGTT CTTCCTCCTG TCCAAAGAGA TGTTCAACCC CTACTACGGG
    CTTTTTGAGT ACTCTGCAAC GGACAACTAC ACACTTCAGA TCAATCCCAA TTCAGGCCTC
    TGTAATGAAG ACCATTTGTC CTATTTCACT TTTATTGGAA GAGTTGCTGG TCTGGCAGTA
    TTTCATGGGA AACTTCTAGA TGGTTTCTTC ATTAGACCGT TTTACAAAAT GATGTTGGGA
    AAGCAGATAA CTCTGAATGA CATGGAATCT GTGGATAGTG AATATTACAA CTCTTTGAAA
    TGGATCTTGG AAAATGATCC TACTGAACTG GACCTCATGT TCTGTATCGA TGAAGAAAAC
    TTTGGGCAGA CCTATCAAGT GGATCTGAAG CCCAATGGGT CAGAAATAAT GGTGACAAAT
    GAAAACAAGA GAGAATATAT TGACTTAGTC ATCCAGTGGA GGTTTGTGAA CAGGGTCCAG
    AAGCAGATGA ACGCCTTCTT GGAGGGATTC ACAGAACTGC TTCCTATTGA TTTGATTAAA
    ATTTTCGATG AAAATGAGCT GGAGTTGCTG ATGTGCGGCC TTGGCGATGT GGACGTGAAC
    GATTGGAGAC AGCATTCTAT TTACAAGAAT GGCTACTGCC CCAACCACCC TGTCATTCAG
    TGGTTCTGGA AGGCTGTGCT GCTCATGGAC GCGGAGAAGC GGATCCGGCT ACTGCAGTTC
    GTCACGGGGA CGTCCCGGGT ACCTATGAAC GGATTCGCCG AACTTTATGG TTCCAATGGT
    CCTCAGCTGT TTACAATAGA GCAATGGGGG AGTCCTGACA AGCTGCCCAG AGCTCACACG
    TGCTTTAATC GCCTTGACTT ACCTCCATAT GAAACCTTTG AAGATTTACG AGAGAAACTC
    CTCATGGCTG TGGAAAACGC TCAAGGATTT GAGGGGGTGG ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe00769
    Clone ID Related Accession (Same CDS sequence) XM_005615375.2
    Accession Version XM_005615375.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2505bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005615375.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGAGCGAC CCTATACATT TAAGGACTTT CTCCTTCGAC CAAGAAGTCA TAAATCTCGA 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCAA AAAATGGCGG TCAAGATGAA
    GAAAACAGTG AACAGAGGGA TGATATGGAG CATGGCTGGG AGGTTGTGGA CTCGAATGAC
    TCGGCCTCTC AGCACCAGGA GGAGCTTCCT CCACCCCCAC TGCCCCCAGG CTGGGAAGAG
    AAGGTGGACA ACCTTGGTCG TACCTATTAT GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGACCAA GCTTGATGGA TGTGTCCTCC GAGTCCGACA GCAACATCAG GCAGATCAAC
    CAGGAGGCCG CGCACCGGCG CTTCCGCTCA CGCAGGCACA TCAGCGAGGA CCTGGAGCCC
    GAGCCTGGCG AGGGTGGAGA CGGCCCAGAG CCTTGGGAGA CCATCTCCGA AGAAGTGAAC
    ATCACGGGAG ACCCCCTCAG TCTGGCGCTG CCCCCACCAC CATCCTCTCC AGTATCTCGG
    ACCAGCCCTC AGGAGCTGTC TGAGGAACTG AGCAGAAGGC TTCAGATCAC TCCAGACTCA
    AATGGGGAGC AATTTGGTTC TTTGATGCAA AGAGAGCCCT CCTCAAGGCT GCGGTCCTGC
    AGTGTCACCG ACGGGGTCGC CGAGCAGGCT CATCTACCAC CGCCATCAGT GGCCTATGCA
    CACACCACGC CGGGCCTACC TTCAGGCTGG GAAGAAAGAA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GACCTATCAT GCAGCTCGCC
    GAAGACGGTG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCCC GTAGCCTCAG CTCGCCGACC GTAACCTTAT CTGCCCCACT GGAGGGTGCC
    AAGGATTCGC CCATACGTCG GGCTGTGAAA GATACCCTTT CCAATCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAAA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA TAACACAAAG
    ACTACAACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ATATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGTTGGG AAGAAAGAAT TCACTTGGAT
    GGCCGAACGT TTTATATTGA CCATAATAGC AAAATTACTC AGTGGGAAGA TCCAAGACTG
    CAGAATCCAG CTATTACTGG TCCGGCTGTT CCTTACTCCA GAGAATTTAA GCAGAAATAT
    GACTACTTTA GGAAGAAATT AAAGAAACCT GCTGATATTC CAAATAGGTT TGAAATGAAA
    CTTCACAGAA ATAACATCTT TGAAGAGTCC TATCGGAGAA TCATGTCTGT GAAAAGACCG
    GATGTCCTGA AAGCTAGACT GTGGATCGAA TTTGAATCAG AGAAAGGTCT TGACTATGGG
    GGTGTGGCCA GAGAGTGGTT CTTCCTCCTG TCCAAAGAGA TGTTCAACCC CTACTACGGG
    CTTTTTGAGT ACTCTGCAAC GGACAACTAC ACACTTCAGA TCAATCCCAA TTCAGGCCTC
    TGTAATGAAG ACCATTTGTC CTATTTCACT TTTATTGGAA GAGTTGCTGG TCTGGCAGTA
    TTTCATGGGA AACTTCTAGA TGGTTTCTTC ATTAGACCGT TTTACAAAAT GATGTTGGGA
    AAGCAGATAA CTCTGAATGA CATGGAATCT GTGGATAGTG AATATTACAA CTCTTTGAAA
    TGGATCTTGG AAAATGATCC TACTGAACTG GACCTCATGT TCTGTATCGA TGAAGAAAAC
    TTTGGGCAGA CCTATCAAGT GGATCTGAAG CCCAATGGGT CAGAAATAAT GGTGACAAAT
    GAAAACAAGA GAGAATATAT TGACTTAGTC ATCCAGTGGA GGTTTGTGAA CAGGGTCCAG
    AAGCAGATGA ACGCCTTCTT GGAGGGATTC ACAGAACTGC TTCCTATTGA TTTGATTAAA
    ATTTTCGATG AAAATGAGCT GGAGTTGCTG ATGTGCGGCC TTGGCGATGT GGACGTGAAC
    GATTGGAGAC AGCATTCTAT TTACAAGAAT GGCTACTGCC CCAACCACCC TGTCATTCAG
    TGGTTCTGGA AGGCTGTGCT GCTCATGGAC GCGGAGAAGC GGATCCGGCT ACTGCAGTTC
    GTCACGGGGA CGTCCCGGGT ACCTATGAAC GGATTCGCCG AACTTTATGG TTCCAATGGT
    CCTCAGCTGT TTACAATAGA GCAATGGGGG AGTCCTGACA AGCTGCCCAG AGCTCACACG
    TGCTTTAATC GCCTTGACTT ACCTCCATAT GAAACCTTTG AAGATTTACG AGAGAAACTC
    CTCATGGCTG TGGAAAACGC TCAAGGATTT GAGGGGGTGG ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005615432.1
    CDS316..2820
    Translation

    Target ORF information:

    RefSeq Version XM_005615375.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005615375.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGAGCGAC CCTATACATT TAAGGACTTT CTCCTTCGAC CAAGAAGTCA TAAATCTCGA 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCAA AAAATGGCGG TCAAGATGAA
    GAAAACAGTG AACAGAGGGA TGATATGGAG CATGGCTGGG AGGTTGTGGA CTCGAATGAC
    TCGGCCTCTC AGCACCAGGA GGAGCTTCCT CCACCCCCAC TGCCCCCAGG CTGGGAAGAG
    AAGGTGGACA ACCTTGGTCG TACCTATTAT GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGACCAA GCTTGATGGA TGTGTCCTCC GAGTCCGACA GCAACATCAG GCAGATCAAC
    CAGGAGGCCG CGCACCGGCG CTTCCGCTCA CGCAGGCACA TCAGCGAGGA CCTGGAGCCC
    GAGCCTGGCG AGGGTGGAGA CGGCCCAGAG CCTTGGGAGA CCATCTCCGA AGAAGTGAAC
    ATCACGGGAG ACCCCCTCAG TCTGGCGCTG CCCCCACCAC CATCCTCTCC AGTATCTCGG
    ACCAGCCCTC AGGAGCTGTC TGAGGAACTG AGCAGAAGGC TTCAGATCAC TCCAGACTCA
    AATGGGGAGC AATTTGGTTC TTTGATGCAA AGAGAGCCCT CCTCAAGGCT GCGGTCCTGC
    AGTGTCACCG ACGGGGTCGC CGAGCAGGCT CATCTACCAC CGCCATCAGT GGCCTATGCA
    CACACCACGC CGGGCCTACC TTCAGGCTGG GAAGAAAGAA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GACCTATCAT GCAGCTCGCC
    GAAGACGGTG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCCC GTAGCCTCAG CTCGCCGACC GTAACCTTAT CTGCCCCACT GGAGGGTGCC
    AAGGATTCGC CCATACGTCG GGCTGTGAAA GATACCCTTT CCAATCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAAA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA TAACACAAAG
    ACTACAACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ATATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGTTGGG AAGAAAGAAT TCACTTGGAT
    GGCCGAACGT TTTATATTGA CCATAATAGC AAAATTACTC AGTGGGAAGA TCCAAGACTG
    CAGAATCCAG CTATTACTGG TCCGGCTGTT CCTTACTCCA GAGAATTTAA GCAGAAATAT
    GACTACTTTA GGAAGAAATT AAAGAAACCT GCTGATATTC CAAATAGGTT TGAAATGAAA
    CTTCACAGAA ATAACATCTT TGAAGAGTCC TATCGGAGAA TCATGTCTGT GAAAAGACCG
    GATGTCCTGA AAGCTAGACT GTGGATCGAA TTTGAATCAG AGAAAGGTCT TGACTATGGG
    GGTGTGGCCA GAGAGTGGTT CTTCCTCCTG TCCAAAGAGA TGTTCAACCC CTACTACGGG
    CTTTTTGAGT ACTCTGCAAC GGACAACTAC ACACTTCAGA TCAATCCCAA TTCAGGCCTC
    TGTAATGAAG ACCATTTGTC CTATTTCACT TTTATTGGAA GAGTTGCTGG TCTGGCAGTA
    TTTCATGGGA AACTTCTAGA TGGTTTCTTC ATTAGACCGT TTTACAAAAT GATGTTGGGA
    AAGCAGATAA CTCTGAATGA CATGGAATCT GTGGATAGTG AATATTACAA CTCTTTGAAA
    TGGATCTTGG AAAATGATCC TACTGAACTG GACCTCATGT TCTGTATCGA TGAAGAAAAC
    TTTGGGCAGA CCTATCAAGT GGATCTGAAG CCCAATGGGT CAGAAATAAT GGTGACAAAT
    GAAAACAAGA GAGAATATAT TGACTTAGTC ATCCAGTGGA GGTTTGTGAA CAGGGTCCAG
    AAGCAGATGA ACGCCTTCTT GGAGGGATTC ACAGAACTGC TTCCTATTGA TTTGATTAAA
    ATTTTCGATG AAAATGAGCT GGAGTTGCTG ATGTGCGGCC TTGGCGATGT GGACGTGAAC
    GATTGGAGAC AGCATTCTAT TTACAAGAAT GGCTACTGCC CCAACCACCC TGTCATTCAG
    TGGTTCTGGA AGGCTGTGCT GCTCATGGAC GCGGAGAAGC GGATCCGGCT ACTGCAGTTC
    GTCACGGGGA CGTCCCGGGT ACCTATGAAC GGATTCGCCG AACTTTATGG TTCCAATGGT
    CCTCAGCTGT TTACAATAGA GCAATGGGGG AGTCCTGACA AGCTGCCCAG AGCTCACACG
    TGCTTTAATC GCCTTGACTT ACCTCCATAT GAAACCTTTG AAGATTTACG AGAGAAACTC
    CTCATGGCTG TGGAAAACGC TCAAGGATTT GAGGGGGTGG ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe285819
    Clone ID Related Accession (Same CDS sequence) XM_022416697.1
    Accession Version XM_022416697.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2757bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGAAAACAG ATTGGTACCC ACAAACCAAG TGTTCCAGCT ATGAATGTGA ATCCGTTATT 
    ACATTCTGGA CACGAGACGA CTTCCTGGGA CAGGTGGACA TACCCCTTAG TCACCTCCCG
    ACTGAAGATC CGACCATGGA GCGACCCTAT ACATTTAAGG ACTTTCTCCT TCGACCAAGA
    AGTCATAAAT CTCGAGTTAA GGGATTTTTG CGATTGAAAA TGGCCTATAT GCCAAAAAAT
    GGCGGTCAAG ATGAAGAAAA CAGTGAACAG AGGGATGATA TGGAGCATGG CTGGGAGGTT
    GTGGACTCGA ATGACTCGGC CTCTCAGCAC CAGGAGGAGC TTCCTCCACC CCCACTGCCC
    CCAGGCTGGG AAGAGAAGGT GGACAACCTT GGTCGTACCT ATTATGTCAA CCACAACAAC
    CGGACCACCC AGTGGCACCG ACCAAGCTTG ATGGATGTGT CCTCCGAGTC CGACAGCAAC
    ATCAGGCAGA TCAACCAGGA GGCCGCGCAC CGGCGCTTCC GCTCACGCAG GCACATCAGC
    GAGGACCTGG AGCCCGAGCC TGGCGAGGGT GGAGACGGCC CAGAGCCTTG GGAGACCATC
    TCCGAAGAAG TGAACATCAC GGGAGACCCC CTCAGTCTGG CGCTGCCCCC ACCACCATCC
    TCTCCAGTAT CTCGGACCAG CCCTCAGGAG CTGTCTGAGG AACTGAGCAG AAGGCTTCAG
    ATCACTCCAG ACTCAAATGG GGAGCAATTT GGTTCTTTGA TGCAAAGAGA GCCCTCCTCA
    AGGCTGCGGT CCTGCAGTGT CACCGACGGG GTCGCCGAGC AGGCTCATCT ACCACCGCCG
    AGTGTCCCAA CTAGGAGAGC GCGGTCATCC ACCGTCACGG GTGGTGAGGA GCCAACGCCA
    TCAGTGGCCT ATGCACACAC CACGCCGGGC CTACCTTCAG GCTGGGAAGA AAGAAAAGAT
    GCTAAGGGAC GCACATACTA TGTCAATCAT AACAATCGAA CCACAACTTG GACTCGACCT
    ATCATGCAGC TCGCCGAAGA CGGTGCGTCC GGATCCGCCA CAAACAGTAA CAACCACCTA
    ATCGAGCCTC AGATCCGCCG GCCCCGTAGC CTCAGCTCGC CGACCGTAAC CTTATCTGCC
    CCACTGGAGG GTGCCAAGGA TTCGCCCATA CGTCGGGCTG TGAAAGATAC CCTTTCCAAT
    CCACAGTCCC CACAGCCATC ACCTTACAAC TCCCCCAAAC CACAACACAA AGTCACACAG
    AGCTTCCTGC CGCCCGGCTG GGAAATGAGG ATAGCGCCCA ACGGCCGGCC CTTCTTCATT
    GACCATAACA CAAAGACTAC AACGTGGGAA GATCCACGCC TGAAATTTCC AGTACATATG
    CGGTCAAAGG CATCCTTAAA CCCCAATGAC CTTGGCCCTC TTCCTCCTGG TTGGGAAGAA
    AGAATTCACT TGGATGGCCG AACGTTTTAT ATTGACCATA GCAGCCAGGT GTTATGTGGA
    GAGAATGATG CCAGAGAATC AGTGCCCTCA TACGATAGCA AAATTACTCA GTGGGAAGAT
    CCAAGACTGC AGAATCCAGC TATTACTGGT CCGAAGGCTG TTCCTTACTC CAGAGAATTT
    AAGCAGAAAT ATGACTACTT TAGGAAGAAA TTAAAGAAAC CTGCTGATAT TCCAAATAGG
    TTTGAAATGA AACTTCACAG AAATAACATC TTTGAAGAGT CCTATCGGAG AATCATGTCT
    GTGAAAAGAC CGGATGTCCT GAAAGCTAGA CTGTGGATCG AATTTGAATC AGAGAAAGGT
    CTTGACTATG GGGGTGTGGC CAGAGAGTGG TTCTTCCTCC TGTCCAAAGA GATGTTCAAC
    CCCTACTACG GGCTTTTTGA GTACTCTGCA ACGGACAACT ACACACTTCA GATCAATCCC
    AATTCAGGCC TCTGTAATGA AGACCATTTG TCCTATTTCA CTTTTATTGG AAGAGTTGCT
    GGTCTGGCAG TATTTCATGG GAAACTTCTA GATGGTTTCT TCATTAGACC GTTTTACAAA
    ATGATGTTGG GAAAGCAGAT AACTCTGAAT GACATGGAAT CTGTGGATAG TGAATATTAC
    AACTCTTTGA AATGGATCTT GGAAAATGAT CCTACTGAAC TGGACCTCAT GTTCTGTATC
    GATGAAGAAA ACTTTGGGCA GACCTATCAA GTGGATCTGA AGCCCAATGG GTCAGAAATA
    ATGGTGACAA ATGAAAACAA GAGAGAATAT ATTGACTTAG TCATCCAGTG GAGGTTTGTG
    AACAGGGTCC AGAAGCAGAT GAACGCCTTC TTGGAGGGAT TCACAGAACT GCTTCCTATT
    GATTTGATTA AAATTTTCGA TGAAAATGAG CTGGAGTTGC TGATGTGCGG CCTTGGCGAT
    GTGGACGTGA ACGATTGGAG ACAGCATTCT ATTTACAAGA ATGGCTACTG CCCCAACCAC
    CCTGTCATTC AGTGGTTCTG GAAGGCTGTG CTGCTCATGG ACGCGGAGAA GCGGATCCGG
    CTACTGCAGT TCGTCACGGG GACGTCCCGG GTACCTATGA ACGGATTCGC CGAACTTTAT
    GGTTCCAATG GTCCTCAGCT GTTTACAATA GAGCAATGGG GGAGTCCTGA CAAGCTGCCC
    AGAGCTCACA CGTGCTTTAA TCGCCTTGAC TTACCTCCAT ATGAAACCTT TGAAGATTTA
    CGAGAGAAAC TCCTCATGGC TGTGGAAAAC GCTCAAGGAT TTGAGGGGGT GGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022272405.1
    CDS144..2900
    Translation

    Target ORF information:

    RefSeq Version XM_022416697.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022416697.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGAAAACAG ATTGGTACCC ACAAACCAAG TGTTCCAGCT ATGAATGTGA ATCCGTTATT 
    ACATTCTGGA CACGAGACGA CTTCCTGGGA CAGGTGGACA TACCCCTTAG TCACCTCCCG
    ACTGAAGATC CGACCATGGA GCGACCCTAT ACATTTAAGG ACTTTCTCCT TCGACCAAGA
    AGTCATAAAT CTCGAGTTAA GGGATTTTTG CGATTGAAAA TGGCCTATAT GCCAAAAAAT
    GGCGGTCAAG ATGAAGAAAA CAGTGAACAG AGGGATGATA TGGAGCATGG CTGGGAGGTT
    GTGGACTCGA ATGACTCGGC CTCTCAGCAC CAGGAGGAGC TTCCTCCACC CCCACTGCCC
    CCAGGCTGGG AAGAGAAGGT GGACAACCTT GGTCGTACCT ATTATGTCAA CCACAACAAC
    CGGACCACCC AGTGGCACCG ACCAAGCTTG ATGGATGTGT CCTCCGAGTC CGACAGCAAC
    ATCAGGCAGA TCAACCAGGA GGCCGCGCAC CGGCGCTTCC GCTCACGCAG GCACATCAGC
    GAGGACCTGG AGCCCGAGCC TGGCGAGGGT GGAGACGGCC CAGAGCCTTG GGAGACCATC
    TCCGAAGAAG TGAACATCAC GGGAGACCCC CTCAGTCTGG CGCTGCCCCC ACCACCATCC
    TCTCCAGTAT CTCGGACCAG CCCTCAGGAG CTGTCTGAGG AACTGAGCAG AAGGCTTCAG
    ATCACTCCAG ACTCAAATGG GGAGCAATTT GGTTCTTTGA TGCAAAGAGA GCCCTCCTCA
    AGGCTGCGGT CCTGCAGTGT CACCGACGGG GTCGCCGAGC AGGCTCATCT ACCACCGCCG
    AGTGTCCCAA CTAGGAGAGC GCGGTCATCC ACCGTCACGG GTGGTGAGGA GCCAACGCCA
    TCAGTGGCCT ATGCACACAC CACGCCGGGC CTACCTTCAG GCTGGGAAGA AAGAAAAGAT
    GCTAAGGGAC GCACATACTA TGTCAATCAT AACAATCGAA CCACAACTTG GACTCGACCT
    ATCATGCAGC TCGCCGAAGA CGGTGCGTCC GGATCCGCCA CAAACAGTAA CAACCACCTA
    ATCGAGCCTC AGATCCGCCG GCCCCGTAGC CTCAGCTCGC CGACCGTAAC CTTATCTGCC
    CCACTGGAGG GTGCCAAGGA TTCGCCCATA CGTCGGGCTG TGAAAGATAC CCTTTCCAAT
    CCACAGTCCC CACAGCCATC ACCTTACAAC TCCCCCAAAC CACAACACAA AGTCACACAG
    AGCTTCCTGC CGCCCGGCTG GGAAATGAGG ATAGCGCCCA ACGGCCGGCC CTTCTTCATT
    GACCATAACA CAAAGACTAC AACGTGGGAA GATCCACGCC TGAAATTTCC AGTACATATG
    CGGTCAAAGG CATCCTTAAA CCCCAATGAC CTTGGCCCTC TTCCTCCTGG TTGGGAAGAA
    AGAATTCACT TGGATGGCCG AACGTTTTAT ATTGACCATA GCAGCCAGGT GTTATGTGGA
    GAGAATGATG CCAGAGAATC AGTGCCCTCA TACGATAGCA AAATTACTCA GTGGGAAGAT
    CCAAGACTGC AGAATCCAGC TATTACTGGT CCGAAGGCTG TTCCTTACTC CAGAGAATTT
    AAGCAGAAAT ATGACTACTT TAGGAAGAAA TTAAAGAAAC CTGCTGATAT TCCAAATAGG
    TTTGAAATGA AACTTCACAG AAATAACATC TTTGAAGAGT CCTATCGGAG AATCATGTCT
    GTGAAAAGAC CGGATGTCCT GAAAGCTAGA CTGTGGATCG AATTTGAATC AGAGAAAGGT
    CTTGACTATG GGGGTGTGGC CAGAGAGTGG TTCTTCCTCC TGTCCAAAGA GATGTTCAAC
    CCCTACTACG GGCTTTTTGA GTACTCTGCA ACGGACAACT ACACACTTCA GATCAATCCC
    AATTCAGGCC TCTGTAATGA AGACCATTTG TCCTATTTCA CTTTTATTGG AAGAGTTGCT
    GGTCTGGCAG TATTTCATGG GAAACTTCTA GATGGTTTCT TCATTAGACC GTTTTACAAA
    ATGATGTTGG GAAAGCAGAT AACTCTGAAT GACATGGAAT CTGTGGATAG TGAATATTAC
    AACTCTTTGA AATGGATCTT GGAAAATGAT CCTACTGAAC TGGACCTCAT GTTCTGTATC
    GATGAAGAAA ACTTTGGGCA GACCTATCAA GTGGATCTGA AGCCCAATGG GTCAGAAATA
    ATGGTGACAA ATGAAAACAA GAGAGAATAT ATTGACTTAG TCATCCAGTG GAGGTTTGTG
    AACAGGGTCC AGAAGCAGAT GAACGCCTTC TTGGAGGGAT TCACAGAACT GCTTCCTATT
    GATTTGATTA AAATTTTCGA TGAAAATGAG CTGGAGTTGC TGATGTGCGG CCTTGGCGAT
    GTGGACGTGA ACGATTGGAG ACAGCATTCT ATTTACAAGA ATGGCTACTG CCCCAACCAC
    CCTGTCATTC AGTGGTTCTG GAAGGCTGTG CTGCTCATGG ACGCGGAGAA GCGGATCCGG
    CTACTGCAGT TCGTCACGGG GACGTCCCGG GTACCTATGA ACGGATTCGC CGAACTTTAT
    GGTTCCAATG GTCCTCAGCT GTTTACAATA GAGCAATGGG GGAGTCCTGA CAAGCTGCCC
    AGAGCTCACA CGTGCTTTAA TCGCCTTGAC TTACCTCCAT ATGAAACCTT TGAAGATTTA
    CGAGAGAAAC TCCTCATGGC TGTGGAAAAC GCTCAAGGAT TTGAGGGGGT GGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe34411
    Clone ID Related Accession (Same CDS sequence) XM_014108785.2 , XM_014108785.1
    Accession Version XM_014108785.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2883bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014108785.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAGCCTTG AGGACAATTA CAGTGATCCG TATGTGAAAC TTTCATTGTA CGTAGCAGAT 
    GAGAATAGAG AACTCGCTTT GGTACAGACA AAAACCATTA AAAAGACGCT GAACCCAAAG
    TGGAATGAAG AATTTTATTT TAGAGTAAAC CCATCTAATC ACAGACTCCT GTTTGAAGTA
    TTTGATGAAA ACAGATTGAC ACGAGACGAC TTCCTGGGAC AGGTGGACAT ACCCCTTAGT
    CACCTCCCGA CTGAAGATCC GACCATGGAG CGACCCTATA CATTTAAGGA CTTTCTCCTT
    CGACCAAGAA GTCATAAATC TCGAGTTAAG GGATTTTTGC GATTGAAAAT GGCCTATATG
    CCAAAAAATG GCGGTCAAGA TGAAGAAAAC AGTGAACAGA GGGATGATAT GGAGCATGGC
    TGGGAGGTTG TGGACTCGAA TGACTCGGCC TCTCAGCACC AGGAGGAGCT TCCTCCACCC
    CCACTGCCCC CAGGCTGGGA AGAGAAGGTG GACAACCTTG GTCGTACCTA TTATGTCAAC
    CACAACAACC GGACCACCCA GTGGCACCGA CCAAGCTTGA TGGATGTGTC CTCCGAGTCC
    GACAGCAACA TCAGGCAGAT CAACCAGGAG GCCGCGCACC GGCGCTTCCG CTCACGCAGG
    CACATCAGCG AGGACCTGGA GCCCGAGCCT GGCGAGGGTG GAGACGGCCC AGAGCCTTGG
    GAGACCATCT CCGAAGAAGT GAACATCACG GGAGACCCCC TCAGTCTGGC GCTGCCCCCA
    CCACCATCCT CTCCAGTATC TCGGACCAGC CCTCAGGAGC TGTCTGAGGA ACTGAGCAGA
    AGGCTTCAGA TCACTCCAGA CTCAAATGGG GAGCAATTTG GTTCTTTGAT GCAAAGAGAG
    CCCTCCTCAA GGCTGCGGTC CTGCAGTGTC ACCGACGGGG TCGCCGAGCA GGCTCATCTA
    CCACCGCCGA GTGTCCCAAC TAGGAGAGCG CGGTCATCCA CCGTCACGGG TGGTGAGGAG
    CCAACGCCAT CAGTGGCCTA TGCACACACC ACGCCGGGCC TACCTTCAGG CTGGGAAGAA
    AGAAAAGATG CTAAGGGACG CACATACTAT GTCAATCATA ACAATCGAAC CACAACTTGG
    ACTCGACCTA TCATGCAGCT CGCCGAAGAC GGTGCGTCCG GATCCGCCAC AAACAGTAAC
    AACCACCTAA TCGAGCCTCA GATCCGCCGG CCCCGTAGCC TCAGCTCGCC GACCGTAACC
    TTATCTGCCC CACTGGAGGG TGCCAAGGAT TCGCCCATAC GTCGGGCTGT GAAAGATACC
    CTTTCCAATC CACAGTCCCC ACAGCCATCA CCTTACAACT CCCCCAAACC ACAACACAAA
    GTCACACAGA GCTTCCTGCC GCCCGGCTGG GAAATGAGGA TAGCGCCCAA CGGCCGGCCC
    TTCTTCATTG ACCATAACAC AAAGACTACA ACGTGGGAAG ATCCACGCCT GAAATTTCCA
    GTACATATGC GGTCAAAGGC ATCCTTAAAC CCCAATGACC TTGGCCCTCT TCCTCCTGGT
    TGGGAAGAAA GAATTCACTT GGATGGCCGA ACGTTTTATA TTGACCATAG CAGCCAGGTG
    TTATGTGGAG AGAATGATGC CAGAGAATCA GTGCCCTCAT ACGATAGCAA AATTACTCAG
    TGGGAAGATC CAAGACTGCA GAATCCAGCT ATTACTGGTC CGGCTGTTCC TTACTCCAGA
    GAATTTAAGC AGAAATATGA CTACTTTAGG AAGAAATTAA AGAAACCTGC TGATATTCCA
    AATAGGTTTG AAATGAAACT TCACAGAAAT AACATCTTTG AAGAGTCCTA TCGGAGAATC
    ATGTCTGTGA AAAGACCGGA TGTCCTGAAA GCTAGACTGT GGATCGAATT TGAATCAGAG
    AAAGGTCTTG ACTATGGGGG TGTGGCCAGA GAGTGGTTCT TCCTCCTGTC CAAAGAGATG
    TTCAACCCCT ACTACGGGCT TTTTGAGTAC TCTGCAACGG ACAACTACAC ACTTCAGATC
    AATCCCAATT CAGGCCTCTG TAATGAAGAC CATTTGTCCT ATTTCACTTT TATTGGAAGA
    GTTGCTGGTC TGGCAGTATT TCATGGGAAA CTTCTAGATG GTTTCTTCAT TAGACCGTTT
    TACAAAATGA TGTTGGGAAA GCAGATAACT CTGAATGACA TGGAATCTGT GGATAGTGAA
    TATTACAACT CTTTGAAATG GATCTTGGAA AATGATCCTA CTGAACTGGA CCTCATGTTC
    TGTATCGATG AAGAAAACTT TGGGCAGACC TATCAAGTGG ATCTGAAGCC CAATGGGTCA
    GAAATAATGG TGACAAATGA AAACAAGAGA GAATATATTG ACTTAGTCAT CCAGTGGAGG
    TTTGTGAACA GGGTCCAGAA GCAGATGAAC GCCTTCTTGG AGGGATTCAC AGAACTGCTT
    CCTATTGATT TGATTAAAAT TTTCGATGAA AATGAGCTGG AGTTGCTGAT GTGCGGCCTT
    GGCGATGTGG ACGTGAACGA TTGGAGACAG CATTCTATTT ACAAGAATGG CTACTGCCCC
    AACCACCCTG TCATTCAGTG GTTCTGGAAG GCTGTGCTGC TCATGGACGC GGAGAAGCGG
    ATCCGGCTAC TGCAGTTCGT CACGGGGACG TCCCGGGTAC CTATGAACGG ATTCGCCGAA
    CTTTATGGTT CCAATGGTCC TCAGCTGTTT ACAATAGAGC AATGGGGGAG TCCTGACAAG
    CTGCCCAGAG CTCACACGTG CTTTAATCGC CTTGACTTAC CTCCATATGA AACCTTTGAA
    GATTTACGAG AGAAACTCCT CATGGCTGTG GAAAACGCTC AAGGATTTGA GGGGGTGGAT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013964260.1
    CDS209..3091
    Translation

    Target ORF information:

    RefSeq Version XM_014108785.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014108785.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAGCCTTG AGGACAATTA CAGTGATCCG TATGTGAAAC TTTCATTGTA CGTAGCAGAT 
    GAGAATAGAG AACTCGCTTT GGTACAGACA AAAACCATTA AAAAGACGCT GAACCCAAAG
    TGGAATGAAG AATTTTATTT TAGAGTAAAC CCATCTAATC ACAGACTCCT GTTTGAAGTA
    TTTGATGAAA ACAGATTGAC ACGAGACGAC TTCCTGGGAC AGGTGGACAT ACCCCTTAGT
    CACCTCCCGA CTGAAGATCC GACCATGGAG CGACCCTATA CATTTAAGGA CTTTCTCCTT
    CGACCAAGAA GTCATAAATC TCGAGTTAAG GGATTTTTGC GATTGAAAAT GGCCTATATG
    CCAAAAAATG GCGGTCAAGA TGAAGAAAAC AGTGAACAGA GGGATGATAT GGAGCATGGC
    TGGGAGGTTG TGGACTCGAA TGACTCGGCC TCTCAGCACC AGGAGGAGCT TCCTCCACCC
    CCACTGCCCC CAGGCTGGGA AGAGAAGGTG GACAACCTTG GTCGTACCTA TTATGTCAAC
    CACAACAACC GGACCACCCA GTGGCACCGA CCAAGCTTGA TGGATGTGTC CTCCGAGTCC
    GACAGCAACA TCAGGCAGAT CAACCAGGAG GCCGCGCACC GGCGCTTCCG CTCACGCAGG
    CACATCAGCG AGGACCTGGA GCCCGAGCCT GGCGAGGGTG GAGACGGCCC AGAGCCTTGG
    GAGACCATCT CCGAAGAAGT GAACATCACG GGAGACCCCC TCAGTCTGGC GCTGCCCCCA
    CCACCATCCT CTCCAGTATC TCGGACCAGC CCTCAGGAGC TGTCTGAGGA ACTGAGCAGA
    AGGCTTCAGA TCACTCCAGA CTCAAATGGG GAGCAATTTG GTTCTTTGAT GCAAAGAGAG
    CCCTCCTCAA GGCTGCGGTC CTGCAGTGTC ACCGACGGGG TCGCCGAGCA GGCTCATCTA
    CCACCGCCGA GTGTCCCAAC TAGGAGAGCG CGGTCATCCA CCGTCACGGG TGGTGAGGAG
    CCAACGCCAT CAGTGGCCTA TGCACACACC ACGCCGGGCC TACCTTCAGG CTGGGAAGAA
    AGAAAAGATG CTAAGGGACG CACATACTAT GTCAATCATA ACAATCGAAC CACAACTTGG
    ACTCGACCTA TCATGCAGCT CGCCGAAGAC GGTGCGTCCG GATCCGCCAC AAACAGTAAC
    AACCACCTAA TCGAGCCTCA GATCCGCCGG CCCCGTAGCC TCAGCTCGCC GACCGTAACC
    TTATCTGCCC CACTGGAGGG TGCCAAGGAT TCGCCCATAC GTCGGGCTGT GAAAGATACC
    CTTTCCAATC CACAGTCCCC ACAGCCATCA CCTTACAACT CCCCCAAACC ACAACACAAA
    GTCACACAGA GCTTCCTGCC GCCCGGCTGG GAAATGAGGA TAGCGCCCAA CGGCCGGCCC
    TTCTTCATTG ACCATAACAC AAAGACTACA ACGTGGGAAG ATCCACGCCT GAAATTTCCA
    GTACATATGC GGTCAAAGGC ATCCTTAAAC CCCAATGACC TTGGCCCTCT TCCTCCTGGT
    TGGGAAGAAA GAATTCACTT GGATGGCCGA ACGTTTTATA TTGACCATAG CAGCCAGGTG
    TTATGTGGAG AGAATGATGC CAGAGAATCA GTGCCCTCAT ACGATAGCAA AATTACTCAG
    TGGGAAGATC CAAGACTGCA GAATCCAGCT ATTACTGGTC CGGCTGTTCC TTACTCCAGA
    GAATTTAAGC AGAAATATGA CTACTTTAGG AAGAAATTAA AGAAACCTGC TGATATTCCA
    AATAGGTTTG AAATGAAACT TCACAGAAAT AACATCTTTG AAGAGTCCTA TCGGAGAATC
    ATGTCTGTGA AAAGACCGGA TGTCCTGAAA GCTAGACTGT GGATCGAATT TGAATCAGAG
    AAAGGTCTTG ACTATGGGGG TGTGGCCAGA GAGTGGTTCT TCCTCCTGTC CAAAGAGATG
    TTCAACCCCT ACTACGGGCT TTTTGAGTAC TCTGCAACGG ACAACTACAC ACTTCAGATC
    AATCCCAATT CAGGCCTCTG TAATGAAGAC CATTTGTCCT ATTTCACTTT TATTGGAAGA
    GTTGCTGGTC TGGCAGTATT TCATGGGAAA CTTCTAGATG GTTTCTTCAT TAGACCGTTT
    TACAAAATGA TGTTGGGAAA GCAGATAACT CTGAATGACA TGGAATCTGT GGATAGTGAA
    TATTACAACT CTTTGAAATG GATCTTGGAA AATGATCCTA CTGAACTGGA CCTCATGTTC
    TGTATCGATG AAGAAAACTT TGGGCAGACC TATCAAGTGG ATCTGAAGCC CAATGGGTCA
    GAAATAATGG TGACAAATGA AAACAAGAGA GAATATATTG ACTTAGTCAT CCAGTGGAGG
    TTTGTGAACA GGGTCCAGAA GCAGATGAAC GCCTTCTTGG AGGGATTCAC AGAACTGCTT
    CCTATTGATT TGATTAAAAT TTTCGATGAA AATGAGCTGG AGTTGCTGAT GTGCGGCCTT
    GGCGATGTGG ACGTGAACGA TTGGAGACAG CATTCTATTT ACAAGAATGG CTACTGCCCC
    AACCACCCTG TCATTCAGTG GTTCTGGAAG GCTGTGCTGC TCATGGACGC GGAGAAGCGG
    ATCCGGCTAC TGCAGTTCGT CACGGGGACG TCCCGGGTAC CTATGAACGG ATTCGCCGAA
    CTTTATGGTT CCAATGGTCC TCAGCTGTTT ACAATAGAGC AATGGGGGAG TCCTGACAAG
    CTGCCCAGAG CTCACACGTG CTTTAATCGC CTTGACTTAC CTCCATATGA AACCTTTGAA
    GATTTACGAG AGAAACTCCT CATGGCTGTG GAAAACGCTC AAGGATTTGA GGGGGTGGAT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe34411
    Clone ID Related Accession (Same CDS sequence) XM_014108785.2 , XM_014108785.1
    Accession Version XM_014108785.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2883bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726046.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAGCCTTG AGGACAATTA CAGTGATCCG TATGTGAAAC TTTCATTGTA CGTAGCAGAT 
    GAGAATAGAG AACTCGCTTT GGTACAGACA AAAACCATTA AAAAGACGCT GAACCCAAAG
    TGGAATGAAG AATTTTATTT TAGAGTAAAC CCATCTAATC ACAGACTCCT GTTTGAAGTA
    TTTGATGAAA ACAGATTGAC ACGAGACGAC TTCCTGGGAC AGGTGGACAT ACCCCTTAGT
    CACCTCCCGA CTGAAGATCC GACCATGGAG CGACCCTATA CATTTAAGGA CTTTCTCCTT
    CGACCAAGAA GTCATAAATC TCGAGTTAAG GGATTTTTGC GATTGAAAAT GGCCTATATG
    CCAAAAAATG GCGGTCAAGA TGAAGAAAAC AGTGAACAGA GGGATGATAT GGAGCATGGC
    TGGGAGGTTG TGGACTCGAA TGACTCGGCC TCTCAGCACC AGGAGGAGCT TCCTCCACCC
    CCACTGCCCC CAGGCTGGGA AGAGAAGGTG GACAACCTTG GTCGTACCTA TTATGTCAAC
    CACAACAACC GGACCACCCA GTGGCACCGA CCAAGCTTGA TGGATGTGTC CTCCGAGTCC
    GACAGCAACA TCAGGCAGAT CAACCAGGAG GCCGCGCACC GGCGCTTCCG CTCACGCAGG
    CACATCAGCG AGGACCTGGA GCCCGAGCCT GGCGAGGGTG GAGACGGCCC AGAGCCTTGG
    GAGACCATCT CCGAAGAAGT GAACATCACG GGAGACCCCC TCAGTCTGGC GCTGCCCCCA
    CCACCATCCT CTCCAGTATC TCGGACCAGC CCTCAGGAGC TGTCTGAGGA ACTGAGCAGA
    AGGCTTCAGA TCACTCCAGA CTCAAATGGG GAGCAATTTG GTTCTTTGAT GCAAAGAGAG
    CCCTCCTCAA GGCTGCGGTC CTGCAGTGTC ACCGACGGGG TCGCCGAGCA GGCTCATCTA
    CCACCGCCGA GTGTCCCAAC TAGGAGAGCG CGGTCATCCA CCGTCACGGG TGGTGAGGAG
    CCAACGCCAT CAGTGGCCTA TGCACACACC ACGCCGGGCC TACCTTCAGG CTGGGAAGAA
    AGAAAAGATG CTAAGGGACG CACATACTAT GTCAATCATA ACAATCGAAC CACAACTTGG
    ACTCGACCTA TCATGCAGCT CGCCGAAGAC GGTGCGTCCG GATCCGCCAC AAACAGTAAC
    AACCACCTAA TCGAGCCTCA GATCCGCCGG CCCCGTAGCC TCAGCTCGCC GACCGTAACC
    TTATCTGCCC CACTGGAGGG TGCCAAGGAT TCGCCCATAC GTCGGGCTGT GAAAGATACC
    CTTTCCAATC CACAGTCCCC ACAGCCATCA CCTTACAACT CCCCCAAACC ACAACACAAA
    GTCACACAGA GCTTCCTGCC GCCCGGCTGG GAAATGAGGA TAGCGCCCAA CGGCCGGCCC
    TTCTTCATTG ACCATAACAC AAAGACTACA ACGTGGGAAG ATCCACGCCT GAAATTTCCA
    GTACATATGC GGTCAAAGGC ATCCTTAAAC CCCAATGACC TTGGCCCTCT TCCTCCTGGT
    TGGGAAGAAA GAATTCACTT GGATGGCCGA ACGTTTTATA TTGACCATAG CAGCCAGGTG
    TTATGTGGAG AGAATGATGC CAGAGAATCA GTGCCCTCAT ACGATAGCAA AATTACTCAG
    TGGGAAGATC CAAGACTGCA GAATCCAGCT ATTACTGGTC CGGCTGTTCC TTACTCCAGA
    GAATTTAAGC AGAAATATGA CTACTTTAGG AAGAAATTAA AGAAACCTGC TGATATTCCA
    AATAGGTTTG AAATGAAACT TCACAGAAAT AACATCTTTG AAGAGTCCTA TCGGAGAATC
    ATGTCTGTGA AAAGACCGGA TGTCCTGAAA GCTAGACTGT GGATCGAATT TGAATCAGAG
    AAAGGTCTTG ACTATGGGGG TGTGGCCAGA GAGTGGTTCT TCCTCCTGTC CAAAGAGATG
    TTCAACCCCT ACTACGGGCT TTTTGAGTAC TCTGCAACGG ACAACTACAC ACTTCAGATC
    AATCCCAATT CAGGCCTCTG TAATGAAGAC CATTTGTCCT ATTTCACTTT TATTGGAAGA
    GTTGCTGGTC TGGCAGTATT TCATGGGAAA CTTCTAGATG GTTTCTTCAT TAGACCGTTT
    TACAAAATGA TGTTGGGAAA GCAGATAACT CTGAATGACA TGGAATCTGT GGATAGTGAA
    TATTACAACT CTTTGAAATG GATCTTGGAA AATGATCCTA CTGAACTGGA CCTCATGTTC
    TGTATCGATG AAGAAAACTT TGGGCAGACC TATCAAGTGG ATCTGAAGCC CAATGGGTCA
    GAAATAATGG TGACAAATGA AAACAAGAGA GAATATATTG ACTTAGTCAT CCAGTGGAGG
    TTTGTGAACA GGGTCCAGAA GCAGATGAAC GCCTTCTTGG AGGGATTCAC AGAACTGCTT
    CCTATTGATT TGATTAAAAT TTTCGATGAA AATGAGCTGG AGTTGCTGAT GTGCGGCCTT
    GGCGATGTGG ACGTGAACGA TTGGAGACAG CATTCTATTT ACAAGAATGG CTACTGCCCC
    AACCACCCTG TCATTCAGTG GTTCTGGAAG GCTGTGCTGC TCATGGACGC GGAGAAGCGG
    ATCCGGCTAC TGCAGTTCGT CACGGGGACG TCCCGGGTAC CTATGAACGG ATTCGCCGAA
    CTTTATGGTT CCAATGGTCC TCAGCTGTTT ACAATAGAGC AATGGGGGAG TCCTGACAAG
    CTGCCCAGAG CTCACACGTG CTTTAATCGC CTTGACTTAC CTCCATATGA AACCTTTGAA
    GATTTACGAG AGAAACTCCT CATGGCTGTG GAAAACGCTC AAGGATTTGA GGGGGTGGAT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013964260.1
    CDS224..3106
    Translation

    Target ORF information:

    RefSeq Version XM_014108785.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014108785.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAGCCTTG AGGACAATTA CAGTGATCCG TATGTGAAAC TTTCATTGTA CGTAGCAGAT 
    GAGAATAGAG AACTCGCTTT GGTACAGACA AAAACCATTA AAAAGACGCT GAACCCAAAG
    TGGAATGAAG AATTTTATTT TAGAGTAAAC CCATCTAATC ACAGACTCCT GTTTGAAGTA
    TTTGATGAAA ACAGATTGAC ACGAGACGAC TTCCTGGGAC AGGTGGACAT ACCCCTTAGT
    CACCTCCCGA CTGAAGATCC GACCATGGAG CGACCCTATA CATTTAAGGA CTTTCTCCTT
    CGACCAAGAA GTCATAAATC TCGAGTTAAG GGATTTTTGC GATTGAAAAT GGCCTATATG
    CCAAAAAATG GCGGTCAAGA TGAAGAAAAC AGTGAACAGA GGGATGATAT GGAGCATGGC
    TGGGAGGTTG TGGACTCGAA TGACTCGGCC TCTCAGCACC AGGAGGAGCT TCCTCCACCC
    CCACTGCCCC CAGGCTGGGA AGAGAAGGTG GACAACCTTG GTCGTACCTA TTATGTCAAC
    CACAACAACC GGACCACCCA GTGGCACCGA CCAAGCTTGA TGGATGTGTC CTCCGAGTCC
    GACAGCAACA TCAGGCAGAT CAACCAGGAG GCCGCGCACC GGCGCTTCCG CTCACGCAGG
    CACATCAGCG AGGACCTGGA GCCCGAGCCT GGCGAGGGTG GAGACGGCCC AGAGCCTTGG
    GAGACCATCT CCGAAGAAGT GAACATCACG GGAGACCCCC TCAGTCTGGC GCTGCCCCCA
    CCACCATCCT CTCCAGTATC TCGGACCAGC CCTCAGGAGC TGTCTGAGGA ACTGAGCAGA
    AGGCTTCAGA TCACTCCAGA CTCAAATGGG GAGCAATTTG GTTCTTTGAT GCAAAGAGAG
    CCCTCCTCAA GGCTGCGGTC CTGCAGTGTC ACCGACGGGG TCGCCGAGCA GGCTCATCTA
    CCACCGCCGA GTGTCCCAAC TAGGAGAGCG CGGTCATCCA CCGTCACGGG TGGTGAGGAG
    CCAACGCCAT CAGTGGCCTA TGCACACACC ACGCCGGGCC TACCTTCAGG CTGGGAAGAA
    AGAAAAGATG CTAAGGGACG CACATACTAT GTCAATCATA ACAATCGAAC CACAACTTGG
    ACTCGACCTA TCATGCAGCT CGCCGAAGAC GGTGCGTCCG GATCCGCCAC AAACAGTAAC
    AACCACCTAA TCGAGCCTCA GATCCGCCGG CCCCGTAGCC TCAGCTCGCC GACCGTAACC
    TTATCTGCCC CACTGGAGGG TGCCAAGGAT TCGCCCATAC GTCGGGCTGT GAAAGATACC
    CTTTCCAATC CACAGTCCCC ACAGCCATCA CCTTACAACT CCCCCAAACC ACAACACAAA
    GTCACACAGA GCTTCCTGCC GCCCGGCTGG GAAATGAGGA TAGCGCCCAA CGGCCGGCCC
    TTCTTCATTG ACCATAACAC AAAGACTACA ACGTGGGAAG ATCCACGCCT GAAATTTCCA
    GTACATATGC GGTCAAAGGC ATCCTTAAAC CCCAATGACC TTGGCCCTCT TCCTCCTGGT
    TGGGAAGAAA GAATTCACTT GGATGGCCGA ACGTTTTATA TTGACCATAG CAGCCAGGTG
    TTATGTGGAG AGAATGATGC CAGAGAATCA GTGCCCTCAT ACGATAGCAA AATTACTCAG
    TGGGAAGATC CAAGACTGCA GAATCCAGCT ATTACTGGTC CGGCTGTTCC TTACTCCAGA
    GAATTTAAGC AGAAATATGA CTACTTTAGG AAGAAATTAA AGAAACCTGC TGATATTCCA
    AATAGGTTTG AAATGAAACT TCACAGAAAT AACATCTTTG AAGAGTCCTA TCGGAGAATC
    ATGTCTGTGA AAAGACCGGA TGTCCTGAAA GCTAGACTGT GGATCGAATT TGAATCAGAG
    AAAGGTCTTG ACTATGGGGG TGTGGCCAGA GAGTGGTTCT TCCTCCTGTC CAAAGAGATG
    TTCAACCCCT ACTACGGGCT TTTTGAGTAC TCTGCAACGG ACAACTACAC ACTTCAGATC
    AATCCCAATT CAGGCCTCTG TAATGAAGAC CATTTGTCCT ATTTCACTTT TATTGGAAGA
    GTTGCTGGTC TGGCAGTATT TCATGGGAAA CTTCTAGATG GTTTCTTCAT TAGACCGTTT
    TACAAAATGA TGTTGGGAAA GCAGATAACT CTGAATGACA TGGAATCTGT GGATAGTGAA
    TATTACAACT CTTTGAAATG GATCTTGGAA AATGATCCTA CTGAACTGGA CCTCATGTTC
    TGTATCGATG AAGAAAACTT TGGGCAGACC TATCAAGTGG ATCTGAAGCC CAATGGGTCA
    GAAATAATGG TGACAAATGA AAACAAGAGA GAATATATTG ACTTAGTCAT CCAGTGGAGG
    TTTGTGAACA GGGTCCAGAA GCAGATGAAC GCCTTCTTGG AGGGATTCAC AGAACTGCTT
    CCTATTGATT TGATTAAAAT TTTCGATGAA AATGAGCTGG AGTTGCTGAT GTGCGGCCTT
    GGCGATGTGG ACGTGAACGA TTGGAGACAG CATTCTATTT ACAAGAATGG CTACTGCCCC
    AACCACCCTG TCATTCAGTG GTTCTGGAAG GCTGTGCTGC TCATGGACGC GGAGAAGCGG
    ATCCGGCTAC TGCAGTTCGT CACGGGGACG TCCCGGGTAC CTATGAACGG ATTCGCCGAA
    CTTTATGGTT CCAATGGTCC TCAGCTGTTT ACAATAGAGC AATGGGGGAG TCCTGACAAG
    CTGCCCAGAG CTCACACGTG CTTTAATCGC CTTGACTTAC CTCCATATGA AACCTTTGAA
    GATTTACGAG AGAAACTCCT CATGGCTGTG GAAAACGCTC AAGGATTTGA GGGGGTGGAT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe34412
    Clone ID Related Accession (Same CDS sequence) XM_014108939.2 , XM_014108971.1 , XM_014109029.1 , XM_014108971.2 , XM_014109014.1 , XM_014108939.1
    Accession Version XM_014108939.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2622bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014108939.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGCGAC CCTATACATT TAAGGACTTT CTCCTTCGAC CAAGAAGTCA TAAATCTCGA 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCAA AAAATGGCGG TCAAGATGAA
    GAAAACAGTG AACAGAGGGA TGATATGGAG CATGGCTGGG AGGTTGTGGA CTCGAATGAC
    TCGGCCTCTC AGCACCAGGA GGAGCTTCCT CCACCCCCAC TGCCCCCAGG CTGGGAAGAG
    AAGGTGGACA ACCTTGGTCG TACCTATTAT GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGACCAA GCTTGATGGA TGTGTCCTCC GAGTCCGACA GCAACATCAG GCAGATCAAC
    CAGGAGGCCG CGCACCGGCG CTTCCGCTCA CGCAGGCACA TCAGCGAGGA CCTGGAGCCC
    GAGCCTGGCG AGGGTGGAGA CGGCCCAGAG CCTTGGGAGA CCATCTCCGA AGAAGTGAAC
    ATCACGGGAG ACCCCCTCAG TCTGGCGCTG CCCCCACCAC CATCCTCTCC AGTATCTCGG
    ACCAGCCCTC AGGAGCTGTC TGAGGAACTG AGCAGAAGGC TTCAGATCAC TCCAGACTCA
    AATGGGGAGC AATTTGGTTC TTTGATGCAA AGAGAGCCCT CCTCAAGGCT GCGGTCCTGC
    AGTGTCACCG ACGGGGTCGC CGAGCAGGCT CATCTACCAC CGCCGAGTGT CCCAACTAGG
    AGAGCGCGGT CATCCACCGT CACGGGTGGT GAGGAGCCAA CGCCATCAGT GGCCTATGCA
    CACACCACGC CGGGCCTACC TTCAGGCTGG GAAGAAAGAA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GACCTATCAT GCAGCTCGCC
    GAAGACGGTG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCCC GTAGCCTCAG CTCGCCGACC GTAACCTTAT CTGCCCCACT GGAGGGTGCC
    AAGGATTCGC CCATACGTCG GGCTGTGAAA GATACCCTTT CCAATCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAAA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA TAACACAAAG
    ACTACAACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ATATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGTTGGG AAGAAAGAAT TCACTTGGAT
    GGCCGAACGT TTTATATTGA CCATAGCAGC CAGGTGTTAT GTGGAGAGAA TGATGCCAGA
    GAATCAGTGC CCTCATACGA TAGCAAAATT ACTCAGTGGG AAGATCCAAG ACTGCAGAAT
    CCAGCTATTA CTGGTCCGAA GGCTGTTCCT TACTCCAGAG AATTTAAGCA GAAATATGAC
    TACTTTAGGA AGAAATTAAA GAAACCTGCT GATATTCCAA ATAGGTTTGA AATGAAACTT
    CACAGAAATA ACATCTTTGA AGAGTCCTAT CGGAGAATCA TGTCTGTGAA AAGACCGGAT
    GTCCTGAAAG CTAGACTGTG GATCGAATTT GAATCAGAGA AAGGTCTTGA CTATGGGGGT
    GTGGCCAGAG AGTGGTTCTT CCTCCTGTCC AAAGAGATGT TCAACCCCTA CTACGGGCTT
    TTTGAGTACT CTGCAACGGA CAACTACACA CTTCAGATCA ATCCCAATTC AGGCCTCTGT
    AATGAAGACC ATTTGTCCTA TTTCACTTTT ATTGGAAGAG TTGCTGGTCT GGCAGTATTT
    CATGGGAAAC TTCTAGATGG TTTCTTCATT AGACCGTTTT ACAAAATGAT GTTGGGAAAG
    CAGATAACTC TGAATGACAT GGAATCTGTG GATAGTGAAT ATTACAACTC TTTGAAATGG
    ATCTTGGAAA ATGATCCTAC TGAACTGGAC CTCATGTTCT GTATCGATGA AGAAAACTTT
    GGGCAGACCT ATCAAGTGGA TCTGAAGCCC AATGGGTCAG AAATAATGGT GACAAATGAA
    AACAAGAGAG AATATATTGA CTTAGTCATC CAGTGGAGGT TTGTGAACAG GGTCCAGAAG
    CAGATGAACG CCTTCTTGGA GGGATTCACA GAACTGCTTC CTATTGATTT GATTAAAATT
    TTCGATGAAA ATGAGCTGGA GTTGCTGATG TGCGGCCTTG GCGATGTGGA CGTGAACGAT
    TGGAGACAGC ATTCTATTTA CAAGAATGGC TACTGCCCCA ACCACCCTGT CATTCAGTGG
    TTCTGGAAGG CTGTGCTGCT CATGGACGCG GAGAAGCGGA TCCGGCTACT GCAGTTCGTC
    ACGGGGACGT CCCGGGTACC TATGAACGGA TTCGCCGAAC TTTATGGTTC CAATGGTCCT
    CAGCTGTTTA CAATAGAGCA ATGGGGGAGT CCTGACAAGC TGCCCAGAGC TCACACGTGC
    TTTAATCGCC TTGACTTACC TCCATATGAA ACCTTTGAAG ATTTACGAGA GAAACTCCTC
    ATGGCTGTGG AAAACGCTCA AGGATTTGAG GGGGTGGATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013964414.1
    CDS91..2712
    Translation

    Target ORF information:

    RefSeq Version XM_014108939.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014108939.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGCGAC CCTATACATT TAAGGACTTT CTCCTTCGAC CAAGAAGTCA TAAATCTCGA 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCAA AAAATGGCGG TCAAGATGAA
    GAAAACAGTG AACAGAGGGA TGATATGGAG CATGGCTGGG AGGTTGTGGA CTCGAATGAC
    TCGGCCTCTC AGCACCAGGA GGAGCTTCCT CCACCCCCAC TGCCCCCAGG CTGGGAAGAG
    AAGGTGGACA ACCTTGGTCG TACCTATTAT GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGACCAA GCTTGATGGA TGTGTCCTCC GAGTCCGACA GCAACATCAG GCAGATCAAC
    CAGGAGGCCG CGCACCGGCG CTTCCGCTCA CGCAGGCACA TCAGCGAGGA CCTGGAGCCC
    GAGCCTGGCG AGGGTGGAGA CGGCCCAGAG CCTTGGGAGA CCATCTCCGA AGAAGTGAAC
    ATCACGGGAG ACCCCCTCAG TCTGGCGCTG CCCCCACCAC CATCCTCTCC AGTATCTCGG
    ACCAGCCCTC AGGAGCTGTC TGAGGAACTG AGCAGAAGGC TTCAGATCAC TCCAGACTCA
    AATGGGGAGC AATTTGGTTC TTTGATGCAA AGAGAGCCCT CCTCAAGGCT GCGGTCCTGC
    AGTGTCACCG ACGGGGTCGC CGAGCAGGCT CATCTACCAC CGCCGAGTGT CCCAACTAGG
    AGAGCGCGGT CATCCACCGT CACGGGTGGT GAGGAGCCAA CGCCATCAGT GGCCTATGCA
    CACACCACGC CGGGCCTACC TTCAGGCTGG GAAGAAAGAA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GACCTATCAT GCAGCTCGCC
    GAAGACGGTG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCCC GTAGCCTCAG CTCGCCGACC GTAACCTTAT CTGCCCCACT GGAGGGTGCC
    AAGGATTCGC CCATACGTCG GGCTGTGAAA GATACCCTTT CCAATCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAAA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA TAACACAAAG
    ACTACAACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ATATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGTTGGG AAGAAAGAAT TCACTTGGAT
    GGCCGAACGT TTTATATTGA CCATAGCAGC CAGGTGTTAT GTGGAGAGAA TGATGCCAGA
    GAATCAGTGC CCTCATACGA TAGCAAAATT ACTCAGTGGG AAGATCCAAG ACTGCAGAAT
    CCAGCTATTA CTGGTCCGAA GGCTGTTCCT TACTCCAGAG AATTTAAGCA GAAATATGAC
    TACTTTAGGA AGAAATTAAA GAAACCTGCT GATATTCCAA ATAGGTTTGA AATGAAACTT
    CACAGAAATA ACATCTTTGA AGAGTCCTAT CGGAGAATCA TGTCTGTGAA AAGACCGGAT
    GTCCTGAAAG CTAGACTGTG GATCGAATTT GAATCAGAGA AAGGTCTTGA CTATGGGGGT
    GTGGCCAGAG AGTGGTTCTT CCTCCTGTCC AAAGAGATGT TCAACCCCTA CTACGGGCTT
    TTTGAGTACT CTGCAACGGA CAACTACACA CTTCAGATCA ATCCCAATTC AGGCCTCTGT
    AATGAAGACC ATTTGTCCTA TTTCACTTTT ATTGGAAGAG TTGCTGGTCT GGCAGTATTT
    CATGGGAAAC TTCTAGATGG TTTCTTCATT AGACCGTTTT ACAAAATGAT GTTGGGAAAG
    CAGATAACTC TGAATGACAT GGAATCTGTG GATAGTGAAT ATTACAACTC TTTGAAATGG
    ATCTTGGAAA ATGATCCTAC TGAACTGGAC CTCATGTTCT GTATCGATGA AGAAAACTTT
    GGGCAGACCT ATCAAGTGGA TCTGAAGCCC AATGGGTCAG AAATAATGGT GACAAATGAA
    AACAAGAGAG AATATATTGA CTTAGTCATC CAGTGGAGGT TTGTGAACAG GGTCCAGAAG
    CAGATGAACG CCTTCTTGGA GGGATTCACA GAACTGCTTC CTATTGATTT GATTAAAATT
    TTCGATGAAA ATGAGCTGGA GTTGCTGATG TGCGGCCTTG GCGATGTGGA CGTGAACGAT
    TGGAGACAGC ATTCTATTTA CAAGAATGGC TACTGCCCCA ACCACCCTGT CATTCAGTGG
    TTCTGGAAGG CTGTGCTGCT CATGGACGCG GAGAAGCGGA TCCGGCTACT GCAGTTCGTC
    ACGGGGACGT CCCGGGTACC TATGAACGGA TTCGCCGAAC TTTATGGTTC CAATGGTCCT
    CAGCTGTTTA CAATAGAGCA ATGGGGGAGT CCTGACAAGC TGCCCAGAGC TCACACGTGC
    TTTAATCGCC TTGACTTACC TCCATATGAA ACCTTTGAAG ATTTACGAGA GAAACTCCTC
    ATGGCTGTGG AAAACGCTCA AGGATTTGAG GGGGTGGATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe34412
    Clone ID Related Accession (Same CDS sequence) XM_014108939.2 , XM_014108971.1 , XM_014109029.1 , XM_014108971.2 , XM_014109014.1 , XM_014108939.1
    Accession Version XM_014108971.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2622bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726046.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGCGAC CCTATACATT TAAGGACTTT CTCCTTCGAC CAAGAAGTCA TAAATCTCGA 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCAA AAAATGGCGG TCAAGATGAA
    GAAAACAGTG AACAGAGGGA TGATATGGAG CATGGCTGGG AGGTTGTGGA CTCGAATGAC
    TCGGCCTCTC AGCACCAGGA GGAGCTTCCT CCACCCCCAC TGCCCCCAGG CTGGGAAGAG
    AAGGTGGACA ACCTTGGTCG TACCTATTAT GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGACCAA GCTTGATGGA TGTGTCCTCC GAGTCCGACA GCAACATCAG GCAGATCAAC
    CAGGAGGCCG CGCACCGGCG CTTCCGCTCA CGCAGGCACA TCAGCGAGGA CCTGGAGCCC
    GAGCCTGGCG AGGGTGGAGA CGGCCCAGAG CCTTGGGAGA CCATCTCCGA AGAAGTGAAC
    ATCACGGGAG ACCCCCTCAG TCTGGCGCTG CCCCCACCAC CATCCTCTCC AGTATCTCGG
    ACCAGCCCTC AGGAGCTGTC TGAGGAACTG AGCAGAAGGC TTCAGATCAC TCCAGACTCA
    AATGGGGAGC AATTTGGTTC TTTGATGCAA AGAGAGCCCT CCTCAAGGCT GCGGTCCTGC
    AGTGTCACCG ACGGGGTCGC CGAGCAGGCT CATCTACCAC CGCCGAGTGT CCCAACTAGG
    AGAGCGCGGT CATCCACCGT CACGGGTGGT GAGGAGCCAA CGCCATCAGT GGCCTATGCA
    CACACCACGC CGGGCCTACC TTCAGGCTGG GAAGAAAGAA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GACCTATCAT GCAGCTCGCC
    GAAGACGGTG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCCC GTAGCCTCAG CTCGCCGACC GTAACCTTAT CTGCCCCACT GGAGGGTGCC
    AAGGATTCGC CCATACGTCG GGCTGTGAAA GATACCCTTT CCAATCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAAA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA TAACACAAAG
    ACTACAACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ATATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGTTGGG AAGAAAGAAT TCACTTGGAT
    GGCCGAACGT TTTATATTGA CCATAGCAGC CAGGTGTTAT GTGGAGAGAA TGATGCCAGA
    GAATCAGTGC CCTCATACGA TAGCAAAATT ACTCAGTGGG AAGATCCAAG ACTGCAGAAT
    CCAGCTATTA CTGGTCCGAA GGCTGTTCCT TACTCCAGAG AATTTAAGCA GAAATATGAC
    TACTTTAGGA AGAAATTAAA GAAACCTGCT GATATTCCAA ATAGGTTTGA AATGAAACTT
    CACAGAAATA ACATCTTTGA AGAGTCCTAT CGGAGAATCA TGTCTGTGAA AAGACCGGAT
    GTCCTGAAAG CTAGACTGTG GATCGAATTT GAATCAGAGA AAGGTCTTGA CTATGGGGGT
    GTGGCCAGAG AGTGGTTCTT CCTCCTGTCC AAAGAGATGT TCAACCCCTA CTACGGGCTT
    TTTGAGTACT CTGCAACGGA CAACTACACA CTTCAGATCA ATCCCAATTC AGGCCTCTGT
    AATGAAGACC ATTTGTCCTA TTTCACTTTT ATTGGAAGAG TTGCTGGTCT GGCAGTATTT
    CATGGGAAAC TTCTAGATGG TTTCTTCATT AGACCGTTTT ACAAAATGAT GTTGGGAAAG
    CAGATAACTC TGAATGACAT GGAATCTGTG GATAGTGAAT ATTACAACTC TTTGAAATGG
    ATCTTGGAAA ATGATCCTAC TGAACTGGAC CTCATGTTCT GTATCGATGA AGAAAACTTT
    GGGCAGACCT ATCAAGTGGA TCTGAAGCCC AATGGGTCAG AAATAATGGT GACAAATGAA
    AACAAGAGAG AATATATTGA CTTAGTCATC CAGTGGAGGT TTGTGAACAG GGTCCAGAAG
    CAGATGAACG CCTTCTTGGA GGGATTCACA GAACTGCTTC CTATTGATTT GATTAAAATT
    TTCGATGAAA ATGAGCTGGA GTTGCTGATG TGCGGCCTTG GCGATGTGGA CGTGAACGAT
    TGGAGACAGC ATTCTATTTA CAAGAATGGC TACTGCCCCA ACCACCCTGT CATTCAGTGG
    TTCTGGAAGG CTGTGCTGCT CATGGACGCG GAGAAGCGGA TCCGGCTACT GCAGTTCGTC
    ACGGGGACGT CCCGGGTACC TATGAACGGA TTCGCCGAAC TTTATGGTTC CAATGGTCCT
    CAGCTGTTTA CAATAGAGCA ATGGGGGAGT CCTGACAAGC TGCCCAGAGC TCACACGTGC
    TTTAATCGCC TTGACTTACC TCCATATGAA ACCTTTGAAG ATTTACGAGA GAAACTCCTC
    ATGGCTGTGG AAAACGCTCA AGGATTTGAG GGGGTGGATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013964446.1
    CDS227..2848
    Translation

    Target ORF information:

    RefSeq Version XM_014108971.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014108971.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGCGAC CCTATACATT TAAGGACTTT CTCCTTCGAC CAAGAAGTCA TAAATCTCGA 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCAA AAAATGGCGG TCAAGATGAA
    GAAAACAGTG AACAGAGGGA TGATATGGAG CATGGCTGGG AGGTTGTGGA CTCGAATGAC
    TCGGCCTCTC AGCACCAGGA GGAGCTTCCT CCACCCCCAC TGCCCCCAGG CTGGGAAGAG
    AAGGTGGACA ACCTTGGTCG TACCTATTAT GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGACCAA GCTTGATGGA TGTGTCCTCC GAGTCCGACA GCAACATCAG GCAGATCAAC
    CAGGAGGCCG CGCACCGGCG CTTCCGCTCA CGCAGGCACA TCAGCGAGGA CCTGGAGCCC
    GAGCCTGGCG AGGGTGGAGA CGGCCCAGAG CCTTGGGAGA CCATCTCCGA AGAAGTGAAC
    ATCACGGGAG ACCCCCTCAG TCTGGCGCTG CCCCCACCAC CATCCTCTCC AGTATCTCGG
    ACCAGCCCTC AGGAGCTGTC TGAGGAACTG AGCAGAAGGC TTCAGATCAC TCCAGACTCA
    AATGGGGAGC AATTTGGTTC TTTGATGCAA AGAGAGCCCT CCTCAAGGCT GCGGTCCTGC
    AGTGTCACCG ACGGGGTCGC CGAGCAGGCT CATCTACCAC CGCCGAGTGT CCCAACTAGG
    AGAGCGCGGT CATCCACCGT CACGGGTGGT GAGGAGCCAA CGCCATCAGT GGCCTATGCA
    CACACCACGC CGGGCCTACC TTCAGGCTGG GAAGAAAGAA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GACCTATCAT GCAGCTCGCC
    GAAGACGGTG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCCC GTAGCCTCAG CTCGCCGACC GTAACCTTAT CTGCCCCACT GGAGGGTGCC
    AAGGATTCGC CCATACGTCG GGCTGTGAAA GATACCCTTT CCAATCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAAA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA TAACACAAAG
    ACTACAACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ATATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGTTGGG AAGAAAGAAT TCACTTGGAT
    GGCCGAACGT TTTATATTGA CCATAGCAGC CAGGTGTTAT GTGGAGAGAA TGATGCCAGA
    GAATCAGTGC CCTCATACGA TAGCAAAATT ACTCAGTGGG AAGATCCAAG ACTGCAGAAT
    CCAGCTATTA CTGGTCCGAA GGCTGTTCCT TACTCCAGAG AATTTAAGCA GAAATATGAC
    TACTTTAGGA AGAAATTAAA GAAACCTGCT GATATTCCAA ATAGGTTTGA AATGAAACTT
    CACAGAAATA ACATCTTTGA AGAGTCCTAT CGGAGAATCA TGTCTGTGAA AAGACCGGAT
    GTCCTGAAAG CTAGACTGTG GATCGAATTT GAATCAGAGA AAGGTCTTGA CTATGGGGGT
    GTGGCCAGAG AGTGGTTCTT CCTCCTGTCC AAAGAGATGT TCAACCCCTA CTACGGGCTT
    TTTGAGTACT CTGCAACGGA CAACTACACA CTTCAGATCA ATCCCAATTC AGGCCTCTGT
    AATGAAGACC ATTTGTCCTA TTTCACTTTT ATTGGAAGAG TTGCTGGTCT GGCAGTATTT
    CATGGGAAAC TTCTAGATGG TTTCTTCATT AGACCGTTTT ACAAAATGAT GTTGGGAAAG
    CAGATAACTC TGAATGACAT GGAATCTGTG GATAGTGAAT ATTACAACTC TTTGAAATGG
    ATCTTGGAAA ATGATCCTAC TGAACTGGAC CTCATGTTCT GTATCGATGA AGAAAACTTT
    GGGCAGACCT ATCAAGTGGA TCTGAAGCCC AATGGGTCAG AAATAATGGT GACAAATGAA
    AACAAGAGAG AATATATTGA CTTAGTCATC CAGTGGAGGT TTGTGAACAG GGTCCAGAAG
    CAGATGAACG CCTTCTTGGA GGGATTCACA GAACTGCTTC CTATTGATTT GATTAAAATT
    TTCGATGAAA ATGAGCTGGA GTTGCTGATG TGCGGCCTTG GCGATGTGGA CGTGAACGAT
    TGGAGACAGC ATTCTATTTA CAAGAATGGC TACTGCCCCA ACCACCCTGT CATTCAGTGG
    TTCTGGAAGG CTGTGCTGCT CATGGACGCG GAGAAGCGGA TCCGGCTACT GCAGTTCGTC
    ACGGGGACGT CCCGGGTACC TATGAACGGA TTCGCCGAAC TTTATGGTTC CAATGGTCCT
    CAGCTGTTTA CAATAGAGCA ATGGGGGAGT CCTGACAAGC TGCCCAGAGC TCACACGTGC
    TTTAATCGCC TTGACTTACC TCCATATGAA ACCTTTGAAG ATTTACGAGA GAAACTCCTC
    ATGGCTGTGG AAAACGCTCA AGGATTTGAG GGGGTGGATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe34412
    Clone ID Related Accession (Same CDS sequence) XM_014108939.2 , XM_014108971.1 , XM_014109029.1 , XM_014108971.2 , XM_014109014.1 , XM_014108939.1
    Accession Version XM_014109029.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2622bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726046.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGCGAC CCTATACATT TAAGGACTTT CTCCTTCGAC CAAGAAGTCA TAAATCTCGA 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCAA AAAATGGCGG TCAAGATGAA
    GAAAACAGTG AACAGAGGGA TGATATGGAG CATGGCTGGG AGGTTGTGGA CTCGAATGAC
    TCGGCCTCTC AGCACCAGGA GGAGCTTCCT CCACCCCCAC TGCCCCCAGG CTGGGAAGAG
    AAGGTGGACA ACCTTGGTCG TACCTATTAT GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGACCAA GCTTGATGGA TGTGTCCTCC GAGTCCGACA GCAACATCAG GCAGATCAAC
    CAGGAGGCCG CGCACCGGCG CTTCCGCTCA CGCAGGCACA TCAGCGAGGA CCTGGAGCCC
    GAGCCTGGCG AGGGTGGAGA CGGCCCAGAG CCTTGGGAGA CCATCTCCGA AGAAGTGAAC
    ATCACGGGAG ACCCCCTCAG TCTGGCGCTG CCCCCACCAC CATCCTCTCC AGTATCTCGG
    ACCAGCCCTC AGGAGCTGTC TGAGGAACTG AGCAGAAGGC TTCAGATCAC TCCAGACTCA
    AATGGGGAGC AATTTGGTTC TTTGATGCAA AGAGAGCCCT CCTCAAGGCT GCGGTCCTGC
    AGTGTCACCG ACGGGGTCGC CGAGCAGGCT CATCTACCAC CGCCGAGTGT CCCAACTAGG
    AGAGCGCGGT CATCCACCGT CACGGGTGGT GAGGAGCCAA CGCCATCAGT GGCCTATGCA
    CACACCACGC CGGGCCTACC TTCAGGCTGG GAAGAAAGAA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GACCTATCAT GCAGCTCGCC
    GAAGACGGTG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCCC GTAGCCTCAG CTCGCCGACC GTAACCTTAT CTGCCCCACT GGAGGGTGCC
    AAGGATTCGC CCATACGTCG GGCTGTGAAA GATACCCTTT CCAATCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAAA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA TAACACAAAG
    ACTACAACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ATATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGTTGGG AAGAAAGAAT TCACTTGGAT
    GGCCGAACGT TTTATATTGA CCATAGCAGC CAGGTGTTAT GTGGAGAGAA TGATGCCAGA
    GAATCAGTGC CCTCATACGA TAGCAAAATT ACTCAGTGGG AAGATCCAAG ACTGCAGAAT
    CCAGCTATTA CTGGTCCGAA GGCTGTTCCT TACTCCAGAG AATTTAAGCA GAAATATGAC
    TACTTTAGGA AGAAATTAAA GAAACCTGCT GATATTCCAA ATAGGTTTGA AATGAAACTT
    CACAGAAATA ACATCTTTGA AGAGTCCTAT CGGAGAATCA TGTCTGTGAA AAGACCGGAT
    GTCCTGAAAG CTAGACTGTG GATCGAATTT GAATCAGAGA AAGGTCTTGA CTATGGGGGT
    GTGGCCAGAG AGTGGTTCTT CCTCCTGTCC AAAGAGATGT TCAACCCCTA CTACGGGCTT
    TTTGAGTACT CTGCAACGGA CAACTACACA CTTCAGATCA ATCCCAATTC AGGCCTCTGT
    AATGAAGACC ATTTGTCCTA TTTCACTTTT ATTGGAAGAG TTGCTGGTCT GGCAGTATTT
    CATGGGAAAC TTCTAGATGG TTTCTTCATT AGACCGTTTT ACAAAATGAT GTTGGGAAAG
    CAGATAACTC TGAATGACAT GGAATCTGTG GATAGTGAAT ATTACAACTC TTTGAAATGG
    ATCTTGGAAA ATGATCCTAC TGAACTGGAC CTCATGTTCT GTATCGATGA AGAAAACTTT
    GGGCAGACCT ATCAAGTGGA TCTGAAGCCC AATGGGTCAG AAATAATGGT GACAAATGAA
    AACAAGAGAG AATATATTGA CTTAGTCATC CAGTGGAGGT TTGTGAACAG GGTCCAGAAG
    CAGATGAACG CCTTCTTGGA GGGATTCACA GAACTGCTTC CTATTGATTT GATTAAAATT
    TTCGATGAAA ATGAGCTGGA GTTGCTGATG TGCGGCCTTG GCGATGTGGA CGTGAACGAT
    TGGAGACAGC ATTCTATTTA CAAGAATGGC TACTGCCCCA ACCACCCTGT CATTCAGTGG
    TTCTGGAAGG CTGTGCTGCT CATGGACGCG GAGAAGCGGA TCCGGCTACT GCAGTTCGTC
    ACGGGGACGT CCCGGGTACC TATGAACGGA TTCGCCGAAC TTTATGGTTC CAATGGTCCT
    CAGCTGTTTA CAATAGAGCA ATGGGGGAGT CCTGACAAGC TGCCCAGAGC TCACACGTGC
    TTTAATCGCC TTGACTTACC TCCATATGAA ACCTTTGAAG ATTTACGAGA GAAACTCCTC
    ATGGCTGTGG AAAACGCTCA AGGATTTGAG GGGGTGGATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013964504.1
    CDS408..3029
    Translation

    Target ORF information:

    RefSeq Version XM_014109029.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014109029.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGCGAC CCTATACATT TAAGGACTTT CTCCTTCGAC CAAGAAGTCA TAAATCTCGA 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCAA AAAATGGCGG TCAAGATGAA
    GAAAACAGTG AACAGAGGGA TGATATGGAG CATGGCTGGG AGGTTGTGGA CTCGAATGAC
    TCGGCCTCTC AGCACCAGGA GGAGCTTCCT CCACCCCCAC TGCCCCCAGG CTGGGAAGAG
    AAGGTGGACA ACCTTGGTCG TACCTATTAT GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGACCAA GCTTGATGGA TGTGTCCTCC GAGTCCGACA GCAACATCAG GCAGATCAAC
    CAGGAGGCCG CGCACCGGCG CTTCCGCTCA CGCAGGCACA TCAGCGAGGA CCTGGAGCCC
    GAGCCTGGCG AGGGTGGAGA CGGCCCAGAG CCTTGGGAGA CCATCTCCGA AGAAGTGAAC
    ATCACGGGAG ACCCCCTCAG TCTGGCGCTG CCCCCACCAC CATCCTCTCC AGTATCTCGG
    ACCAGCCCTC AGGAGCTGTC TGAGGAACTG AGCAGAAGGC TTCAGATCAC TCCAGACTCA
    AATGGGGAGC AATTTGGTTC TTTGATGCAA AGAGAGCCCT CCTCAAGGCT GCGGTCCTGC
    AGTGTCACCG ACGGGGTCGC CGAGCAGGCT CATCTACCAC CGCCGAGTGT CCCAACTAGG
    AGAGCGCGGT CATCCACCGT CACGGGTGGT GAGGAGCCAA CGCCATCAGT GGCCTATGCA
    CACACCACGC CGGGCCTACC TTCAGGCTGG GAAGAAAGAA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GACCTATCAT GCAGCTCGCC
    GAAGACGGTG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCCC GTAGCCTCAG CTCGCCGACC GTAACCTTAT CTGCCCCACT GGAGGGTGCC
    AAGGATTCGC CCATACGTCG GGCTGTGAAA GATACCCTTT CCAATCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAAA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA TAACACAAAG
    ACTACAACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ATATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGTTGGG AAGAAAGAAT TCACTTGGAT
    GGCCGAACGT TTTATATTGA CCATAGCAGC CAGGTGTTAT GTGGAGAGAA TGATGCCAGA
    GAATCAGTGC CCTCATACGA TAGCAAAATT ACTCAGTGGG AAGATCCAAG ACTGCAGAAT
    CCAGCTATTA CTGGTCCGAA GGCTGTTCCT TACTCCAGAG AATTTAAGCA GAAATATGAC
    TACTTTAGGA AGAAATTAAA GAAACCTGCT GATATTCCAA ATAGGTTTGA AATGAAACTT
    CACAGAAATA ACATCTTTGA AGAGTCCTAT CGGAGAATCA TGTCTGTGAA AAGACCGGAT
    GTCCTGAAAG CTAGACTGTG GATCGAATTT GAATCAGAGA AAGGTCTTGA CTATGGGGGT
    GTGGCCAGAG AGTGGTTCTT CCTCCTGTCC AAAGAGATGT TCAACCCCTA CTACGGGCTT
    TTTGAGTACT CTGCAACGGA CAACTACACA CTTCAGATCA ATCCCAATTC AGGCCTCTGT
    AATGAAGACC ATTTGTCCTA TTTCACTTTT ATTGGAAGAG TTGCTGGTCT GGCAGTATTT
    CATGGGAAAC TTCTAGATGG TTTCTTCATT AGACCGTTTT ACAAAATGAT GTTGGGAAAG
    CAGATAACTC TGAATGACAT GGAATCTGTG GATAGTGAAT ATTACAACTC TTTGAAATGG
    ATCTTGGAAA ATGATCCTAC TGAACTGGAC CTCATGTTCT GTATCGATGA AGAAAACTTT
    GGGCAGACCT ATCAAGTGGA TCTGAAGCCC AATGGGTCAG AAATAATGGT GACAAATGAA
    AACAAGAGAG AATATATTGA CTTAGTCATC CAGTGGAGGT TTGTGAACAG GGTCCAGAAG
    CAGATGAACG CCTTCTTGGA GGGATTCACA GAACTGCTTC CTATTGATTT GATTAAAATT
    TTCGATGAAA ATGAGCTGGA GTTGCTGATG TGCGGCCTTG GCGATGTGGA CGTGAACGAT
    TGGAGACAGC ATTCTATTTA CAAGAATGGC TACTGCCCCA ACCACCCTGT CATTCAGTGG
    TTCTGGAAGG CTGTGCTGCT CATGGACGCG GAGAAGCGGA TCCGGCTACT GCAGTTCGTC
    ACGGGGACGT CCCGGGTACC TATGAACGGA TTCGCCGAAC TTTATGGTTC CAATGGTCCT
    CAGCTGTTTA CAATAGAGCA ATGGGGGAGT CCTGACAAGC TGCCCAGAGC TCACACGTGC
    TTTAATCGCC TTGACTTACC TCCATATGAA ACCTTTGAAG ATTTACGAGA GAAACTCCTC
    ATGGCTGTGG AAAACGCTCA AGGATTTGAG GGGGTGGATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe34412
    Clone ID Related Accession (Same CDS sequence) XM_014108939.2 , XM_014108971.1 , XM_014109029.1 , XM_014108971.2 , XM_014109014.1 , XM_014108939.1
    Accession Version XM_014108971.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2622bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014108971.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGCGAC CCTATACATT TAAGGACTTT CTCCTTCGAC CAAGAAGTCA TAAATCTCGA 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCAA AAAATGGCGG TCAAGATGAA
    GAAAACAGTG AACAGAGGGA TGATATGGAG CATGGCTGGG AGGTTGTGGA CTCGAATGAC
    TCGGCCTCTC AGCACCAGGA GGAGCTTCCT CCACCCCCAC TGCCCCCAGG CTGGGAAGAG
    AAGGTGGACA ACCTTGGTCG TACCTATTAT GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGACCAA GCTTGATGGA TGTGTCCTCC GAGTCCGACA GCAACATCAG GCAGATCAAC
    CAGGAGGCCG CGCACCGGCG CTTCCGCTCA CGCAGGCACA TCAGCGAGGA CCTGGAGCCC
    GAGCCTGGCG AGGGTGGAGA CGGCCCAGAG CCTTGGGAGA CCATCTCCGA AGAAGTGAAC
    ATCACGGGAG ACCCCCTCAG TCTGGCGCTG CCCCCACCAC CATCCTCTCC AGTATCTCGG
    ACCAGCCCTC AGGAGCTGTC TGAGGAACTG AGCAGAAGGC TTCAGATCAC TCCAGACTCA
    AATGGGGAGC AATTTGGTTC TTTGATGCAA AGAGAGCCCT CCTCAAGGCT GCGGTCCTGC
    AGTGTCACCG ACGGGGTCGC CGAGCAGGCT CATCTACCAC CGCCGAGTGT CCCAACTAGG
    AGAGCGCGGT CATCCACCGT CACGGGTGGT GAGGAGCCAA CGCCATCAGT GGCCTATGCA
    CACACCACGC CGGGCCTACC TTCAGGCTGG GAAGAAAGAA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GACCTATCAT GCAGCTCGCC
    GAAGACGGTG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCCC GTAGCCTCAG CTCGCCGACC GTAACCTTAT CTGCCCCACT GGAGGGTGCC
    AAGGATTCGC CCATACGTCG GGCTGTGAAA GATACCCTTT CCAATCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAAA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA TAACACAAAG
    ACTACAACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ATATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGTTGGG AAGAAAGAAT TCACTTGGAT
    GGCCGAACGT TTTATATTGA CCATAGCAGC CAGGTGTTAT GTGGAGAGAA TGATGCCAGA
    GAATCAGTGC CCTCATACGA TAGCAAAATT ACTCAGTGGG AAGATCCAAG ACTGCAGAAT
    CCAGCTATTA CTGGTCCGAA GGCTGTTCCT TACTCCAGAG AATTTAAGCA GAAATATGAC
    TACTTTAGGA AGAAATTAAA GAAACCTGCT GATATTCCAA ATAGGTTTGA AATGAAACTT
    CACAGAAATA ACATCTTTGA AGAGTCCTAT CGGAGAATCA TGTCTGTGAA AAGACCGGAT
    GTCCTGAAAG CTAGACTGTG GATCGAATTT GAATCAGAGA AAGGTCTTGA CTATGGGGGT
    GTGGCCAGAG AGTGGTTCTT CCTCCTGTCC AAAGAGATGT TCAACCCCTA CTACGGGCTT
    TTTGAGTACT CTGCAACGGA CAACTACACA CTTCAGATCA ATCCCAATTC AGGCCTCTGT
    AATGAAGACC ATTTGTCCTA TTTCACTTTT ATTGGAAGAG TTGCTGGTCT GGCAGTATTT
    CATGGGAAAC TTCTAGATGG TTTCTTCATT AGACCGTTTT ACAAAATGAT GTTGGGAAAG
    CAGATAACTC TGAATGACAT GGAATCTGTG GATAGTGAAT ATTACAACTC TTTGAAATGG
    ATCTTGGAAA ATGATCCTAC TGAACTGGAC CTCATGTTCT GTATCGATGA AGAAAACTTT
    GGGCAGACCT ATCAAGTGGA TCTGAAGCCC AATGGGTCAG AAATAATGGT GACAAATGAA
    AACAAGAGAG AATATATTGA CTTAGTCATC CAGTGGAGGT TTGTGAACAG GGTCCAGAAG
    CAGATGAACG CCTTCTTGGA GGGATTCACA GAACTGCTTC CTATTGATTT GATTAAAATT
    TTCGATGAAA ATGAGCTGGA GTTGCTGATG TGCGGCCTTG GCGATGTGGA CGTGAACGAT
    TGGAGACAGC ATTCTATTTA CAAGAATGGC TACTGCCCCA ACCACCCTGT CATTCAGTGG
    TTCTGGAAGG CTGTGCTGCT CATGGACGCG GAGAAGCGGA TCCGGCTACT GCAGTTCGTC
    ACGGGGACGT CCCGGGTACC TATGAACGGA TTCGCCGAAC TTTATGGTTC CAATGGTCCT
    CAGCTGTTTA CAATAGAGCA ATGGGGGAGT CCTGACAAGC TGCCCAGAGC TCACACGTGC
    TTTAATCGCC TTGACTTACC TCCATATGAA ACCTTTGAAG ATTTACGAGA GAAACTCCTC
    ATGGCTGTGG AAAACGCTCA AGGATTTGAG GGGGTGGATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013964446.1
    CDS181..2802
    Translation

    Target ORF information:

    RefSeq Version XM_014108971.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014108971.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGCGAC CCTATACATT TAAGGACTTT CTCCTTCGAC CAAGAAGTCA TAAATCTCGA 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCAA AAAATGGCGG TCAAGATGAA
    GAAAACAGTG AACAGAGGGA TGATATGGAG CATGGCTGGG AGGTTGTGGA CTCGAATGAC
    TCGGCCTCTC AGCACCAGGA GGAGCTTCCT CCACCCCCAC TGCCCCCAGG CTGGGAAGAG
    AAGGTGGACA ACCTTGGTCG TACCTATTAT GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGACCAA GCTTGATGGA TGTGTCCTCC GAGTCCGACA GCAACATCAG GCAGATCAAC
    CAGGAGGCCG CGCACCGGCG CTTCCGCTCA CGCAGGCACA TCAGCGAGGA CCTGGAGCCC
    GAGCCTGGCG AGGGTGGAGA CGGCCCAGAG CCTTGGGAGA CCATCTCCGA AGAAGTGAAC
    ATCACGGGAG ACCCCCTCAG TCTGGCGCTG CCCCCACCAC CATCCTCTCC AGTATCTCGG
    ACCAGCCCTC AGGAGCTGTC TGAGGAACTG AGCAGAAGGC TTCAGATCAC TCCAGACTCA
    AATGGGGAGC AATTTGGTTC TTTGATGCAA AGAGAGCCCT CCTCAAGGCT GCGGTCCTGC
    AGTGTCACCG ACGGGGTCGC CGAGCAGGCT CATCTACCAC CGCCGAGTGT CCCAACTAGG
    AGAGCGCGGT CATCCACCGT CACGGGTGGT GAGGAGCCAA CGCCATCAGT GGCCTATGCA
    CACACCACGC CGGGCCTACC TTCAGGCTGG GAAGAAAGAA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GACCTATCAT GCAGCTCGCC
    GAAGACGGTG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCCC GTAGCCTCAG CTCGCCGACC GTAACCTTAT CTGCCCCACT GGAGGGTGCC
    AAGGATTCGC CCATACGTCG GGCTGTGAAA GATACCCTTT CCAATCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAAA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA TAACACAAAG
    ACTACAACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ATATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGTTGGG AAGAAAGAAT TCACTTGGAT
    GGCCGAACGT TTTATATTGA CCATAGCAGC CAGGTGTTAT GTGGAGAGAA TGATGCCAGA
    GAATCAGTGC CCTCATACGA TAGCAAAATT ACTCAGTGGG AAGATCCAAG ACTGCAGAAT
    CCAGCTATTA CTGGTCCGAA GGCTGTTCCT TACTCCAGAG AATTTAAGCA GAAATATGAC
    TACTTTAGGA AGAAATTAAA GAAACCTGCT GATATTCCAA ATAGGTTTGA AATGAAACTT
    CACAGAAATA ACATCTTTGA AGAGTCCTAT CGGAGAATCA TGTCTGTGAA AAGACCGGAT
    GTCCTGAAAG CTAGACTGTG GATCGAATTT GAATCAGAGA AAGGTCTTGA CTATGGGGGT
    GTGGCCAGAG AGTGGTTCTT CCTCCTGTCC AAAGAGATGT TCAACCCCTA CTACGGGCTT
    TTTGAGTACT CTGCAACGGA CAACTACACA CTTCAGATCA ATCCCAATTC AGGCCTCTGT
    AATGAAGACC ATTTGTCCTA TTTCACTTTT ATTGGAAGAG TTGCTGGTCT GGCAGTATTT
    CATGGGAAAC TTCTAGATGG TTTCTTCATT AGACCGTTTT ACAAAATGAT GTTGGGAAAG
    CAGATAACTC TGAATGACAT GGAATCTGTG GATAGTGAAT ATTACAACTC TTTGAAATGG
    ATCTTGGAAA ATGATCCTAC TGAACTGGAC CTCATGTTCT GTATCGATGA AGAAAACTTT
    GGGCAGACCT ATCAAGTGGA TCTGAAGCCC AATGGGTCAG AAATAATGGT GACAAATGAA
    AACAAGAGAG AATATATTGA CTTAGTCATC CAGTGGAGGT TTGTGAACAG GGTCCAGAAG
    CAGATGAACG CCTTCTTGGA GGGATTCACA GAACTGCTTC CTATTGATTT GATTAAAATT
    TTCGATGAAA ATGAGCTGGA GTTGCTGATG TGCGGCCTTG GCGATGTGGA CGTGAACGAT
    TGGAGACAGC ATTCTATTTA CAAGAATGGC TACTGCCCCA ACCACCCTGT CATTCAGTGG
    TTCTGGAAGG CTGTGCTGCT CATGGACGCG GAGAAGCGGA TCCGGCTACT GCAGTTCGTC
    ACGGGGACGT CCCGGGTACC TATGAACGGA TTCGCCGAAC TTTATGGTTC CAATGGTCCT
    CAGCTGTTTA CAATAGAGCA ATGGGGGAGT CCTGACAAGC TGCCCAGAGC TCACACGTGC
    TTTAATCGCC TTGACTTACC TCCATATGAA ACCTTTGAAG ATTTACGAGA GAAACTCCTC
    ATGGCTGTGG AAAACGCTCA AGGATTTGAG GGGGTGGATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe34412
    Clone ID Related Accession (Same CDS sequence) XM_014108939.2 , XM_014108971.1 , XM_014109029.1 , XM_014108971.2 , XM_014109014.1 , XM_014108939.1
    Accession Version XM_014109014.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2622bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGCGAC CCTATACATT TAAGGACTTT CTCCTTCGAC CAAGAAGTCA TAAATCTCGA 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCAA AAAATGGCGG TCAAGATGAA
    GAAAACAGTG AACAGAGGGA TGATATGGAG CATGGCTGGG AGGTTGTGGA CTCGAATGAC
    TCGGCCTCTC AGCACCAGGA GGAGCTTCCT CCACCCCCAC TGCCCCCAGG CTGGGAAGAG
    AAGGTGGACA ACCTTGGTCG TACCTATTAT GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGACCAA GCTTGATGGA TGTGTCCTCC GAGTCCGACA GCAACATCAG GCAGATCAAC
    CAGGAGGCCG CGCACCGGCG CTTCCGCTCA CGCAGGCACA TCAGCGAGGA CCTGGAGCCC
    GAGCCTGGCG AGGGTGGAGA CGGCCCAGAG CCTTGGGAGA CCATCTCCGA AGAAGTGAAC
    ATCACGGGAG ACCCCCTCAG TCTGGCGCTG CCCCCACCAC CATCCTCTCC AGTATCTCGG
    ACCAGCCCTC AGGAGCTGTC TGAGGAACTG AGCAGAAGGC TTCAGATCAC TCCAGACTCA
    AATGGGGAGC AATTTGGTTC TTTGATGCAA AGAGAGCCCT CCTCAAGGCT GCGGTCCTGC
    AGTGTCACCG ACGGGGTCGC CGAGCAGGCT CATCTACCAC CGCCGAGTGT CCCAACTAGG
    AGAGCGCGGT CATCCACCGT CACGGGTGGT GAGGAGCCAA CGCCATCAGT GGCCTATGCA
    CACACCACGC CGGGCCTACC TTCAGGCTGG GAAGAAAGAA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GACCTATCAT GCAGCTCGCC
    GAAGACGGTG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCCC GTAGCCTCAG CTCGCCGACC GTAACCTTAT CTGCCCCACT GGAGGGTGCC
    AAGGATTCGC CCATACGTCG GGCTGTGAAA GATACCCTTT CCAATCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAAA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA TAACACAAAG
    ACTACAACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ATATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGTTGGG AAGAAAGAAT TCACTTGGAT
    GGCCGAACGT TTTATATTGA CCATAGCAGC CAGGTGTTAT GTGGAGAGAA TGATGCCAGA
    GAATCAGTGC CCTCATACGA TAGCAAAATT ACTCAGTGGG AAGATCCAAG ACTGCAGAAT
    CCAGCTATTA CTGGTCCGAA GGCTGTTCCT TACTCCAGAG AATTTAAGCA GAAATATGAC
    TACTTTAGGA AGAAATTAAA GAAACCTGCT GATATTCCAA ATAGGTTTGA AATGAAACTT
    CACAGAAATA ACATCTTTGA AGAGTCCTAT CGGAGAATCA TGTCTGTGAA AAGACCGGAT
    GTCCTGAAAG CTAGACTGTG GATCGAATTT GAATCAGAGA AAGGTCTTGA CTATGGGGGT
    GTGGCCAGAG AGTGGTTCTT CCTCCTGTCC AAAGAGATGT TCAACCCCTA CTACGGGCTT
    TTTGAGTACT CTGCAACGGA CAACTACACA CTTCAGATCA ATCCCAATTC AGGCCTCTGT
    AATGAAGACC ATTTGTCCTA TTTCACTTTT ATTGGAAGAG TTGCTGGTCT GGCAGTATTT
    CATGGGAAAC TTCTAGATGG TTTCTTCATT AGACCGTTTT ACAAAATGAT GTTGGGAAAG
    CAGATAACTC TGAATGACAT GGAATCTGTG GATAGTGAAT ATTACAACTC TTTGAAATGG
    ATCTTGGAAA ATGATCCTAC TGAACTGGAC CTCATGTTCT GTATCGATGA AGAAAACTTT
    GGGCAGACCT ATCAAGTGGA TCTGAAGCCC AATGGGTCAG AAATAATGGT GACAAATGAA
    AACAAGAGAG AATATATTGA CTTAGTCATC CAGTGGAGGT TTGTGAACAG GGTCCAGAAG
    CAGATGAACG CCTTCTTGGA GGGATTCACA GAACTGCTTC CTATTGATTT GATTAAAATT
    TTCGATGAAA ATGAGCTGGA GTTGCTGATG TGCGGCCTTG GCGATGTGGA CGTGAACGAT
    TGGAGACAGC ATTCTATTTA CAAGAATGGC TACTGCCCCA ACCACCCTGT CATTCAGTGG
    TTCTGGAAGG CTGTGCTGCT CATGGACGCG GAGAAGCGGA TCCGGCTACT GCAGTTCGTC
    ACGGGGACGT CCCGGGTACC TATGAACGGA TTCGCCGAAC TTTATGGTTC CAATGGTCCT
    CAGCTGTTTA CAATAGAGCA ATGGGGGAGT CCTGACAAGC TGCCCAGAGC TCACACGTGC
    TTTAATCGCC TTGACTTACC TCCATATGAA ACCTTTGAAG ATTTACGAGA GAAACTCCTC
    ATGGCTGTGG AAAACGCTCA AGGATTTGAG GGGGTGGATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013964489.1
    CDS316..2937
    Translation

    Target ORF information:

    RefSeq Version XM_014109014.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014109014.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGCGAC CCTATACATT TAAGGACTTT CTCCTTCGAC CAAGAAGTCA TAAATCTCGA 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCAA AAAATGGCGG TCAAGATGAA
    GAAAACAGTG AACAGAGGGA TGATATGGAG CATGGCTGGG AGGTTGTGGA CTCGAATGAC
    TCGGCCTCTC AGCACCAGGA GGAGCTTCCT CCACCCCCAC TGCCCCCAGG CTGGGAAGAG
    AAGGTGGACA ACCTTGGTCG TACCTATTAT GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGACCAA GCTTGATGGA TGTGTCCTCC GAGTCCGACA GCAACATCAG GCAGATCAAC
    CAGGAGGCCG CGCACCGGCG CTTCCGCTCA CGCAGGCACA TCAGCGAGGA CCTGGAGCCC
    GAGCCTGGCG AGGGTGGAGA CGGCCCAGAG CCTTGGGAGA CCATCTCCGA AGAAGTGAAC
    ATCACGGGAG ACCCCCTCAG TCTGGCGCTG CCCCCACCAC CATCCTCTCC AGTATCTCGG
    ACCAGCCCTC AGGAGCTGTC TGAGGAACTG AGCAGAAGGC TTCAGATCAC TCCAGACTCA
    AATGGGGAGC AATTTGGTTC TTTGATGCAA AGAGAGCCCT CCTCAAGGCT GCGGTCCTGC
    AGTGTCACCG ACGGGGTCGC CGAGCAGGCT CATCTACCAC CGCCGAGTGT CCCAACTAGG
    AGAGCGCGGT CATCCACCGT CACGGGTGGT GAGGAGCCAA CGCCATCAGT GGCCTATGCA
    CACACCACGC CGGGCCTACC TTCAGGCTGG GAAGAAAGAA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GACCTATCAT GCAGCTCGCC
    GAAGACGGTG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCCC GTAGCCTCAG CTCGCCGACC GTAACCTTAT CTGCCCCACT GGAGGGTGCC
    AAGGATTCGC CCATACGTCG GGCTGTGAAA GATACCCTTT CCAATCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAAA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA TAACACAAAG
    ACTACAACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ATATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGTTGGG AAGAAAGAAT TCACTTGGAT
    GGCCGAACGT TTTATATTGA CCATAGCAGC CAGGTGTTAT GTGGAGAGAA TGATGCCAGA
    GAATCAGTGC CCTCATACGA TAGCAAAATT ACTCAGTGGG AAGATCCAAG ACTGCAGAAT
    CCAGCTATTA CTGGTCCGAA GGCTGTTCCT TACTCCAGAG AATTTAAGCA GAAATATGAC
    TACTTTAGGA AGAAATTAAA GAAACCTGCT GATATTCCAA ATAGGTTTGA AATGAAACTT
    CACAGAAATA ACATCTTTGA AGAGTCCTAT CGGAGAATCA TGTCTGTGAA AAGACCGGAT
    GTCCTGAAAG CTAGACTGTG GATCGAATTT GAATCAGAGA AAGGTCTTGA CTATGGGGGT
    GTGGCCAGAG AGTGGTTCTT CCTCCTGTCC AAAGAGATGT TCAACCCCTA CTACGGGCTT
    TTTGAGTACT CTGCAACGGA CAACTACACA CTTCAGATCA ATCCCAATTC AGGCCTCTGT
    AATGAAGACC ATTTGTCCTA TTTCACTTTT ATTGGAAGAG TTGCTGGTCT GGCAGTATTT
    CATGGGAAAC TTCTAGATGG TTTCTTCATT AGACCGTTTT ACAAAATGAT GTTGGGAAAG
    CAGATAACTC TGAATGACAT GGAATCTGTG GATAGTGAAT ATTACAACTC TTTGAAATGG
    ATCTTGGAAA ATGATCCTAC TGAACTGGAC CTCATGTTCT GTATCGATGA AGAAAACTTT
    GGGCAGACCT ATCAAGTGGA TCTGAAGCCC AATGGGTCAG AAATAATGGT GACAAATGAA
    AACAAGAGAG AATATATTGA CTTAGTCATC CAGTGGAGGT TTGTGAACAG GGTCCAGAAG
    CAGATGAACG CCTTCTTGGA GGGATTCACA GAACTGCTTC CTATTGATTT GATTAAAATT
    TTCGATGAAA ATGAGCTGGA GTTGCTGATG TGCGGCCTTG GCGATGTGGA CGTGAACGAT
    TGGAGACAGC ATTCTATTTA CAAGAATGGC TACTGCCCCA ACCACCCTGT CATTCAGTGG
    TTCTGGAAGG CTGTGCTGCT CATGGACGCG GAGAAGCGGA TCCGGCTACT GCAGTTCGTC
    ACGGGGACGT CCCGGGTACC TATGAACGGA TTCGCCGAAC TTTATGGTTC CAATGGTCCT
    CAGCTGTTTA CAATAGAGCA ATGGGGGAGT CCTGACAAGC TGCCCAGAGC TCACACGTGC
    TTTAATCGCC TTGACTTACC TCCATATGAA ACCTTTGAAG ATTTACGAGA GAAACTCCTC
    ATGGCTGTGG AAAACGCTCA AGGATTTGAG GGGGTGGATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe34412
    Clone ID Related Accession (Same CDS sequence) XM_014108939.2 , XM_014108971.1 , XM_014109029.1 , XM_014108971.2 , XM_014109014.1 , XM_014108939.1
    Accession Version XM_014108939.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2622bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726046.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGCGAC CCTATACATT TAAGGACTTT CTCCTTCGAC CAAGAAGTCA TAAATCTCGA 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCAA AAAATGGCGG TCAAGATGAA
    GAAAACAGTG AACAGAGGGA TGATATGGAG CATGGCTGGG AGGTTGTGGA CTCGAATGAC
    TCGGCCTCTC AGCACCAGGA GGAGCTTCCT CCACCCCCAC TGCCCCCAGG CTGGGAAGAG
    AAGGTGGACA ACCTTGGTCG TACCTATTAT GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGACCAA GCTTGATGGA TGTGTCCTCC GAGTCCGACA GCAACATCAG GCAGATCAAC
    CAGGAGGCCG CGCACCGGCG CTTCCGCTCA CGCAGGCACA TCAGCGAGGA CCTGGAGCCC
    GAGCCTGGCG AGGGTGGAGA CGGCCCAGAG CCTTGGGAGA CCATCTCCGA AGAAGTGAAC
    ATCACGGGAG ACCCCCTCAG TCTGGCGCTG CCCCCACCAC CATCCTCTCC AGTATCTCGG
    ACCAGCCCTC AGGAGCTGTC TGAGGAACTG AGCAGAAGGC TTCAGATCAC TCCAGACTCA
    AATGGGGAGC AATTTGGTTC TTTGATGCAA AGAGAGCCCT CCTCAAGGCT GCGGTCCTGC
    AGTGTCACCG ACGGGGTCGC CGAGCAGGCT CATCTACCAC CGCCGAGTGT CCCAACTAGG
    AGAGCGCGGT CATCCACCGT CACGGGTGGT GAGGAGCCAA CGCCATCAGT GGCCTATGCA
    CACACCACGC CGGGCCTACC TTCAGGCTGG GAAGAAAGAA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GACCTATCAT GCAGCTCGCC
    GAAGACGGTG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCCC GTAGCCTCAG CTCGCCGACC GTAACCTTAT CTGCCCCACT GGAGGGTGCC
    AAGGATTCGC CCATACGTCG GGCTGTGAAA GATACCCTTT CCAATCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAAA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA TAACACAAAG
    ACTACAACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ATATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGTTGGG AAGAAAGAAT TCACTTGGAT
    GGCCGAACGT TTTATATTGA CCATAGCAGC CAGGTGTTAT GTGGAGAGAA TGATGCCAGA
    GAATCAGTGC CCTCATACGA TAGCAAAATT ACTCAGTGGG AAGATCCAAG ACTGCAGAAT
    CCAGCTATTA CTGGTCCGAA GGCTGTTCCT TACTCCAGAG AATTTAAGCA GAAATATGAC
    TACTTTAGGA AGAAATTAAA GAAACCTGCT GATATTCCAA ATAGGTTTGA AATGAAACTT
    CACAGAAATA ACATCTTTGA AGAGTCCTAT CGGAGAATCA TGTCTGTGAA AAGACCGGAT
    GTCCTGAAAG CTAGACTGTG GATCGAATTT GAATCAGAGA AAGGTCTTGA CTATGGGGGT
    GTGGCCAGAG AGTGGTTCTT CCTCCTGTCC AAAGAGATGT TCAACCCCTA CTACGGGCTT
    TTTGAGTACT CTGCAACGGA CAACTACACA CTTCAGATCA ATCCCAATTC AGGCCTCTGT
    AATGAAGACC ATTTGTCCTA TTTCACTTTT ATTGGAAGAG TTGCTGGTCT GGCAGTATTT
    CATGGGAAAC TTCTAGATGG TTTCTTCATT AGACCGTTTT ACAAAATGAT GTTGGGAAAG
    CAGATAACTC TGAATGACAT GGAATCTGTG GATAGTGAAT ATTACAACTC TTTGAAATGG
    ATCTTGGAAA ATGATCCTAC TGAACTGGAC CTCATGTTCT GTATCGATGA AGAAAACTTT
    GGGCAGACCT ATCAAGTGGA TCTGAAGCCC AATGGGTCAG AAATAATGGT GACAAATGAA
    AACAAGAGAG AATATATTGA CTTAGTCATC CAGTGGAGGT TTGTGAACAG GGTCCAGAAG
    CAGATGAACG CCTTCTTGGA GGGATTCACA GAACTGCTTC CTATTGATTT GATTAAAATT
    TTCGATGAAA ATGAGCTGGA GTTGCTGATG TGCGGCCTTG GCGATGTGGA CGTGAACGAT
    TGGAGACAGC ATTCTATTTA CAAGAATGGC TACTGCCCCA ACCACCCTGT CATTCAGTGG
    TTCTGGAAGG CTGTGCTGCT CATGGACGCG GAGAAGCGGA TCCGGCTACT GCAGTTCGTC
    ACGGGGACGT CCCGGGTACC TATGAACGGA TTCGCCGAAC TTTATGGTTC CAATGGTCCT
    CAGCTGTTTA CAATAGAGCA ATGGGGGAGT CCTGACAAGC TGCCCAGAGC TCACACGTGC
    TTTAATCGCC TTGACTTACC TCCATATGAA ACCTTTGAAG ATTTACGAGA GAAACTCCTC
    ATGGCTGTGG AAAACGCTCA AGGATTTGAG GGGGTGGATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013964414.1
    CDS394..3015
    Translation

    Target ORF information:

    RefSeq Version XM_014108939.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014108939.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGCGAC CCTATACATT TAAGGACTTT CTCCTTCGAC CAAGAAGTCA TAAATCTCGA 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCAA AAAATGGCGG TCAAGATGAA
    GAAAACAGTG AACAGAGGGA TGATATGGAG CATGGCTGGG AGGTTGTGGA CTCGAATGAC
    TCGGCCTCTC AGCACCAGGA GGAGCTTCCT CCACCCCCAC TGCCCCCAGG CTGGGAAGAG
    AAGGTGGACA ACCTTGGTCG TACCTATTAT GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGACCAA GCTTGATGGA TGTGTCCTCC GAGTCCGACA GCAACATCAG GCAGATCAAC
    CAGGAGGCCG CGCACCGGCG CTTCCGCTCA CGCAGGCACA TCAGCGAGGA CCTGGAGCCC
    GAGCCTGGCG AGGGTGGAGA CGGCCCAGAG CCTTGGGAGA CCATCTCCGA AGAAGTGAAC
    ATCACGGGAG ACCCCCTCAG TCTGGCGCTG CCCCCACCAC CATCCTCTCC AGTATCTCGG
    ACCAGCCCTC AGGAGCTGTC TGAGGAACTG AGCAGAAGGC TTCAGATCAC TCCAGACTCA
    AATGGGGAGC AATTTGGTTC TTTGATGCAA AGAGAGCCCT CCTCAAGGCT GCGGTCCTGC
    AGTGTCACCG ACGGGGTCGC CGAGCAGGCT CATCTACCAC CGCCGAGTGT CCCAACTAGG
    AGAGCGCGGT CATCCACCGT CACGGGTGGT GAGGAGCCAA CGCCATCAGT GGCCTATGCA
    CACACCACGC CGGGCCTACC TTCAGGCTGG GAAGAAAGAA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GACCTATCAT GCAGCTCGCC
    GAAGACGGTG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCCC GTAGCCTCAG CTCGCCGACC GTAACCTTAT CTGCCCCACT GGAGGGTGCC
    AAGGATTCGC CCATACGTCG GGCTGTGAAA GATACCCTTT CCAATCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAAA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA TAACACAAAG
    ACTACAACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ATATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGTTGGG AAGAAAGAAT TCACTTGGAT
    GGCCGAACGT TTTATATTGA CCATAGCAGC CAGGTGTTAT GTGGAGAGAA TGATGCCAGA
    GAATCAGTGC CCTCATACGA TAGCAAAATT ACTCAGTGGG AAGATCCAAG ACTGCAGAAT
    CCAGCTATTA CTGGTCCGAA GGCTGTTCCT TACTCCAGAG AATTTAAGCA GAAATATGAC
    TACTTTAGGA AGAAATTAAA GAAACCTGCT GATATTCCAA ATAGGTTTGA AATGAAACTT
    CACAGAAATA ACATCTTTGA AGAGTCCTAT CGGAGAATCA TGTCTGTGAA AAGACCGGAT
    GTCCTGAAAG CTAGACTGTG GATCGAATTT GAATCAGAGA AAGGTCTTGA CTATGGGGGT
    GTGGCCAGAG AGTGGTTCTT CCTCCTGTCC AAAGAGATGT TCAACCCCTA CTACGGGCTT
    TTTGAGTACT CTGCAACGGA CAACTACACA CTTCAGATCA ATCCCAATTC AGGCCTCTGT
    AATGAAGACC ATTTGTCCTA TTTCACTTTT ATTGGAAGAG TTGCTGGTCT GGCAGTATTT
    CATGGGAAAC TTCTAGATGG TTTCTTCATT AGACCGTTTT ACAAAATGAT GTTGGGAAAG
    CAGATAACTC TGAATGACAT GGAATCTGTG GATAGTGAAT ATTACAACTC TTTGAAATGG
    ATCTTGGAAA ATGATCCTAC TGAACTGGAC CTCATGTTCT GTATCGATGA AGAAAACTTT
    GGGCAGACCT ATCAAGTGGA TCTGAAGCCC AATGGGTCAG AAATAATGGT GACAAATGAA
    AACAAGAGAG AATATATTGA CTTAGTCATC CAGTGGAGGT TTGTGAACAG GGTCCAGAAG
    CAGATGAACG CCTTCTTGGA GGGATTCACA GAACTGCTTC CTATTGATTT GATTAAAATT
    TTCGATGAAA ATGAGCTGGA GTTGCTGATG TGCGGCCTTG GCGATGTGGA CGTGAACGAT
    TGGAGACAGC ATTCTATTTA CAAGAATGGC TACTGCCCCA ACCACCCTGT CATTCAGTGG
    TTCTGGAAGG CTGTGCTGCT CATGGACGCG GAGAAGCGGA TCCGGCTACT GCAGTTCGTC
    ACGGGGACGT CCCGGGTACC TATGAACGGA TTCGCCGAAC TTTATGGTTC CAATGGTCCT
    CAGCTGTTTA CAATAGAGCA ATGGGGGAGT CCTGACAAGC TGCCCAGAGC TCACACGTGC
    TTTAATCGCC TTGACTTACC TCCATATGAA ACCTTTGAAG ATTTACGAGA GAAACTCCTC
    ATGGCTGTGG AAAACGCTCA AGGATTTGAG GGGGTGGATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.