NEDD4L cDNA ORF clone, Bos taurus(cattle)

The following NEDD4L gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NEDD4L cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa14469 XM_015460154.2
Latest version!
Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa47560 XM_010799400.2
Latest version!
Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa47560 XM_010819011.3
Latest version!
Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa47561 XM_024984476.1
Latest version!
Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa47561 XM_015469185.1
Latest version!
Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa47561 XM_015469186.1
Latest version!
Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa47561 XM_024984478.1
Latest version!
Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa47561 XM_024984479.1
Latest version!
Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa47561 XM_010799401.2
Latest version!
Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa47561 XM_024984477.1
Latest version!
Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa47561 XM_010819012.3
Latest version!
Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X25, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa47561 XM_015460155.1
Latest version!
Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa47561 XM_005224351.4
Latest version!
Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa47561 XM_015460157.1
Latest version!
Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa47561 XM_005199752.3
Latest version!
Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa47561 XM_015469184.1
Latest version!
Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa47561 XM_015460156.2
Latest version!
Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa230043 XM_024984471.1
Latest version!
Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa230044 XM_024984472.1
Latest version!
Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa230045 XM_024984473.1
Latest version!
Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa230046 XM_024984475.1
Latest version!
Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa230047 XM_024984486.1
Latest version!
Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X26, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa88365 XM_015460158.1
Latest version!
Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa88365 XM_005199753.3
Latest version!
Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa88365 XM_015469187.1
Latest version!
Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa88365 XM_002697776.6
Latest version!
Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa88365 XM_024984480.1
Latest version!
Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X20, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa88365 XM_024984481.1
Latest version!
Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X21, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa88366 XM_005199754.3
Latest version!
Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa88366 XM_005224352.4
Latest version!
Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa88366 XM_024984482.1
Latest version!
Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X22, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa88367 XM_015469183.1
Latest version!
Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa88367 XM_015460153.2
Latest version!
Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa88368 XM_024984485.1
Latest version!
Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X24, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa88368 XM_015460159.1
Latest version!
Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa88368 XM_024984483.1
Latest version!
Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X23, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa88368 XM_005224353.4
Latest version!
Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa88368 XM_005199755.3
Latest version!
Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa88368 XM_015469188.1
Latest version!
Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa88369 XM_005224354.4
Latest version!
Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OBa88369 XM_005199756.3
Latest version!
Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OBa88370 XM_005199757.3
Latest version!
Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OBa88370 XM_005224355.4
Latest version!
Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OBa88371 XM_005224356.4
Latest version!
Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OBa88371 XM_005199758.3
Latest version!
Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
View Old Accession Versions >>
OBa88365 XM_002697776.5 Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa88368 XM_005224353.3 Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa88367 XM_015460153.1 Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa47560 XM_010819011.2 Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa47561 XM_015460156.1 Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa47561 XM_010819012.2 Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa88366 XM_005224352.3 Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa47561 XM_005224351.3 Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa88369 XM_005224354.3 Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OBa88370 XM_005224355.3 Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OBa88371 XM_005224356.3 Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OBa14469 XM_015460154.1 Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa14469
    Clone ID Related Accession (Same CDS sequence) XM_015460154.1 , XM_015460154.2
    Accession Version XM_015460154.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2829bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104569.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAACCTTG AGGACAATTA TAGTGATCCA TACGTGAAAC TTTCACTGTA CGTAGCGGAT 
    GAGAATCGGG AACTTGCTTT GGTACAAACA AAAACAATTA AAAAGACGCT GAACCCAAAG
    TGGAATGAAG AATTTTATTT TAGAGTAAAT CCATCTAATC ACAGACTCCT GTTTGAAGTA
    TTTGATGAAA ACAGACTGAC ACGGGACGAC TTCCTGGGAC AGGTGGACAT ACCCCTTAGT
    CATCTCCCGA CAGAAGACCC AACCATGGAG CGACCCTACA CATTTAAGGA CTTTCTCCTC
    CGGCCAAGAA GTCATAAATC TCGGGTTAAG GGATTTTTGC GATTGAAAAT GGCCTATATG
    CCCAAAAACG GAGGCCAAGA TGAAGAGAAC AGTGAGCAGC GGGATGAGAT GGAGCCCGGG
    TGGGAAGTCG TTGACTCCAA TGACTCGGCT TCTCAGCACC AGGAGGAGCT GCCCCCGCCT
    CCGCTGCCCC CCGGGTGGGA AGAGAAGGTG GACAACCTGG GCCGCACGTA CTACGTCAAC
    CACAACAACC GGACCACCCA GTGGCACCGG CCCAGCCTCA TGGACGTGTC GTCCGAGTCC
    GACAGCAACA TCCGGCAGAT CAACCAGGAG GCCGCGCATC GCCGCTTCCG CTCGCGCAGA
    CATATCAGCG AGGACCTGGA GCCGGAGCCC AGCGAGGGCG GGGAGGGCCC CGAGCCTTGG
    GAGACCATTT CAGAGGAAGT GAACATGAGC GGAGACTCGC TCAGCCTGGC GCTGCCCCCG
    CCACCGGCCT CGCCCGTGTC CCGGACGAGC CCCCAGGAGC TGTCCGAGGA GCTGAGCAGA
    AGGCTGCAGG TCACTCCCGA CTCCAACGGC GAGCAGTTTG GGTCTTTGAT TCAGAGAGAG
    CCGTCCTCCA GGCTGAGGTC CTGCAGTGTC ACCGACGCAG TCGCGGAGCA GGCTCACCTC
    CCGCCGCCCA GTGCCCCAGC TGGGAGAGCA CGTTCATCAA CTGTCACTGG TGGTGAGGCG
    CCAACGCCAT CAGTGGCCTA TGTACACACC ACGCCGGGCC TGCCCTCGGG CTGGGAAGAG
    AGGAAAGATG CTAAGGGACG CACATACTAT GTCAATCATA ACAATCGAAC CACAACTTGG
    ACTCGGCCTA TCATGCAGCT CGCAGAAGAC GGCGCGTCCG GATCCGCCAC AAACAGTAAC
    AACCACCTAA TCGAGCCTCA GATCCGCCGG CCTCGTAGCC TCAGCTCGCC AACAGTAACT
    TTATCCGCCC CGCTGGAGGG CGCCAAGGAC TCACCCGTAC GTCGGGCTGT GAAAGATACC
    CTTTCCAACC CACAGTCCCC ACAGCCATCA CCTTACAACT CCCCCAAACC ACAACACAAA
    GTCACACAGA GCTTCCTGCC GCCCGGCTGG GAGATGAGGA TAGCGCCCAA CGGCCGGCCC
    TTCTTCATTG ACCACAACAC AAAGACCACG ACGTGGGAAG ATCCACGCCT GAAATTTCCA
    GTACACATGC GGTCAAAGGC ATCCTTAAAC CCCAATGACC TTGGCCCTCT TCCTCCTGGC
    TGGGAAGAAA GGATCCACTT GGATGGCCGG ACGTTTTACA TTGACCACAA TAGCAAAATT
    ACTCAGTGGG AAGACCCAAG ACTGCAGAAC CCAGCTATTA CTGGTCCGGC TGTTCCTTAC
    TCCAGAGAAT TTAAGCAGAA ATATGACTAC TTTAGAAAGA AATTAAAGAA ACCTGCGGAT
    ATTCCAAATA GGTTTGAAAT GAAACTTCAC AGAAATAACA TATTTGAAGA GTCCTATCGG
    AGAATCATGT CTGTGAAAAG ACCAGATGTC CTGAAAGCTA GGCTGTGGAT TGAGTTTGAA
    TCGGAGAAAG GTCTGGACTA CGGGGGCGTG GCCAGAGAGT GGTTCTTCCT ACTGTCCAAG
    GAGATGTTCA ACCCTTACTA CGGCCTCTTC GAGTATTCGG CAACGGACAA CTACACACTT
    CAGATCAATC CCAATTCAGG CCTCTGTAAT GAAGATCATT TGTCCTATTT CACTTTCATT
    GGAAGAGTTG CTGGGCTGGC AGTATTTCAT GGGAAACTTC TAGATGGTTT CTTCATTCGA
    CCGTTTTATA AAATGATGCT GGGAAAGCAG ATAACTCTGA ATGACATGGA ATCTGTGGAT
    AGCGAATATT ACAACTCTTT GAAATGGATC TTAGAAAATG ACCCAACTGA ACTGGACCTT
    ATGTTCTGCA TTGATGAAGA GAACTTTGGG CAGACATATC AAGTGGATCT GAAGCCCAAC
    GGGTCAGAAA TAATGGTAAC GAACGAAAAC AAGAGAGAGT ACATCGACCT CGTCATCCAG
    TGGAGGTTCG TGAACAGGGT CCAGAAGCAG ATGAACGCCT TCCTGGAGGG ATTCACGGAA
    CTGCTCCCTA TTGATTTGAT TAAAATCTTT GATGAAAATG AACTGGAGTT GCTGATGTGT
    GGCCTTGGCG ACGTGGATGT GAACGACTGG CGACAACATT CTATTTACAA GAATGGCTAC
    TGCCCGAACC ACCCCGTGAT CCAGTGGTTC TGGAAGGCTG TGCTGCTGAT GGACGCCGAG
    AAGCGCATCC GGTTGCTGCA GTTTGTCACA GGGACGTCCC GAGTACCTAT GAATGGATTT
    GCCGAGCTTT ATGGTTCCAA TGGTCCTCAG CTGTTTACAA TAGAGCAATG GGGAAGTCCT
    GAAAAACTGC CCAGAGCTCA CACGTGCTTT AATCGCCTTG ACTTACCTCC GTATGAAACC
    TTCGAAGATT TACGAGAGAA GCTCCTCATG GCTGTGGAAA ACGCCCAAGG CTTCGAGGGG
    GTGGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015315640.1
    CDS227..3055
    Translation

    Target ORF information:

    RefSeq Version XM_015460154.1
    Organism Bos taurus(cattle)
    Definition Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015460154.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAACCTTG AGGACAATTA TAGTGATCCA TACGTGAAAC TTTCACTGTA CGTAGCGGAT 
    GAGAATCGGG AACTTGCTTT GGTACAAACA AAAACAATTA AAAAGACGCT GAACCCAAAG
    TGGAATGAAG AATTTTATTT TAGAGTAAAT CCATCTAATC ACAGACTCCT GTTTGAAGTA
    TTTGATGAAA ACAGACTGAC ACGGGACGAC TTCCTGGGAC AGGTGGACAT ACCCCTTAGT
    CATCTCCCGA CAGAAGACCC AACCATGGAG CGACCCTACA CATTTAAGGA CTTTCTCCTC
    CGGCCAAGAA GTCATAAATC TCGGGTTAAG GGATTTTTGC GATTGAAAAT GGCCTATATG
    CCCAAAAACG GAGGCCAAGA TGAAGAGAAC AGTGAGCAGC GGGATGAGAT GGAGCCCGGG
    TGGGAAGTCG TTGACTCCAA TGACTCGGCT TCTCAGCACC AGGAGGAGCT GCCCCCGCCT
    CCGCTGCCCC CCGGGTGGGA AGAGAAGGTG GACAACCTGG GCCGCACGTA CTACGTCAAC
    CACAACAACC GGACCACCCA GTGGCACCGG CCCAGCCTCA TGGACGTGTC GTCCGAGTCC
    GACAGCAACA TCCGGCAGAT CAACCAGGAG GCCGCGCATC GCCGCTTCCG CTCGCGCAGA
    CATATCAGCG AGGACCTGGA GCCGGAGCCC AGCGAGGGCG GGGAGGGCCC CGAGCCTTGG
    GAGACCATTT CAGAGGAAGT GAACATGAGC GGAGACTCGC TCAGCCTGGC GCTGCCCCCG
    CCACCGGCCT CGCCCGTGTC CCGGACGAGC CCCCAGGAGC TGTCCGAGGA GCTGAGCAGA
    AGGCTGCAGG TCACTCCCGA CTCCAACGGC GAGCAGTTTG GGTCTTTGAT TCAGAGAGAG
    CCGTCCTCCA GGCTGAGGTC CTGCAGTGTC ACCGACGCAG TCGCGGAGCA GGCTCACCTC
    CCGCCGCCCA GTGCCCCAGC TGGGAGAGCA CGTTCATCAA CTGTCACTGG TGGTGAGGCG
    CCAACGCCAT CAGTGGCCTA TGTACACACC ACGCCGGGCC TGCCCTCGGG CTGGGAAGAG
    AGGAAAGATG CTAAGGGACG CACATACTAT GTCAATCATA ACAATCGAAC CACAACTTGG
    ACTCGGCCTA TCATGCAGCT CGCAGAAGAC GGCGCGTCCG GATCCGCCAC AAACAGTAAC
    AACCACCTAA TCGAGCCTCA GATCCGCCGG CCTCGTAGCC TCAGCTCGCC AACAGTAACT
    TTATCCGCCC CGCTGGAGGG CGCCAAGGAC TCACCCGTAC GTCGGGCTGT GAAAGATACC
    CTTTCCAACC CACAGTCCCC ACAGCCATCA CCTTACAACT CCCCCAAACC ACAACACAAA
    GTCACACAGA GCTTCCTGCC GCCCGGCTGG GAGATGAGGA TAGCGCCCAA CGGCCGGCCC
    TTCTTCATTG ACCACAACAC AAAGACCACG ACGTGGGAAG ATCCACGCCT GAAATTTCCA
    GTACACATGC GGTCAAAGGC ATCCTTAAAC CCCAATGACC TTGGCCCTCT TCCTCCTGGC
    TGGGAAGAAA GGATCCACTT GGATGGCCGG ACGTTTTACA TTGACCACAA TAGCAAAATT
    ACTCAGTGGG AAGACCCAAG ACTGCAGAAC CCAGCTATTA CTGGTCCGGC TGTTCCTTAC
    TCCAGAGAAT TTAAGCAGAA ATATGACTAC TTTAGAAAGA AATTAAAGAA ACCTGCGGAT
    ATTCCAAATA GGTTTGAAAT GAAACTTCAC AGAAATAACA TATTTGAAGA GTCCTATCGG
    AGAATCATGT CTGTGAAAAG ACCAGATGTC CTGAAAGCTA GGCTGTGGAT TGAGTTTGAA
    TCGGAGAAAG GTCTGGACTA CGGGGGCGTG GCCAGAGAGT GGTTCTTCCT ACTGTCCAAG
    GAGATGTTCA ACCCTTACTA CGGCCTCTTC GAGTATTCGG CAACGGACAA CTACACACTT
    CAGATCAATC CCAATTCAGG CCTCTGTAAT GAAGATCATT TGTCCTATTT CACTTTCATT
    GGAAGAGTTG CTGGGCTGGC AGTATTTCAT GGGAAACTTC TAGATGGTTT CTTCATTCGA
    CCGTTTTATA AAATGATGCT GGGAAAGCAG ATAACTCTGA ATGACATGGA ATCTGTGGAT
    AGCGAATATT ACAACTCTTT GAAATGGATC TTAGAAAATG ACCCAACTGA ACTGGACCTT
    ATGTTCTGCA TTGATGAAGA GAACTTTGGG CAGACATATC AAGTGGATCT GAAGCCCAAC
    GGGTCAGAAA TAATGGTAAC GAACGAAAAC AAGAGAGAGT ACATCGACCT CGTCATCCAG
    TGGAGGTTCG TGAACAGGGT CCAGAAGCAG ATGAACGCCT TCCTGGAGGG ATTCACGGAA
    CTGCTCCCTA TTGATTTGAT TAAAATCTTT GATGAAAATG AACTGGAGTT GCTGATGTGT
    GGCCTTGGCG ACGTGGATGT GAACGACTGG CGACAACATT CTATTTACAA GAATGGCTAC
    TGCCCGAACC ACCCCGTGAT CCAGTGGTTC TGGAAGGCTG TGCTGCTGAT GGACGCCGAG
    AAGCGCATCC GGTTGCTGCA GTTTGTCACA GGGACGTCCC GAGTACCTAT GAATGGATTT
    GCCGAGCTTT ATGGTTCCAA TGGTCCTCAG CTGTTTACAA TAGAGCAATG GGGAAGTCCT
    GAAAAACTGC CCAGAGCTCA CACGTGCTTT AATCGCCTTG ACTTACCTCC GTATGAAACC
    TTCGAAGATT TACGAGAGAA GCTCCTCATG GCTGTGGAAA ACGCCCAAGG CTTCGAGGGG
    GTGGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa14469
    Clone ID Related Accession (Same CDS sequence) XM_015460154.1 , XM_015460154.2
    Accession Version XM_015460154.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2829bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015460154.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAACCTTG AGGACAATTA TAGTGATCCA TACGTGAAAC TTTCACTGTA CGTAGCGGAT 
    GAGAATCGGG AACTTGCTTT GGTACAAACA AAAACAATTA AAAAGACGCT GAACCCAAAG
    TGGAATGAAG AATTTTATTT TAGAGTAAAT CCATCTAATC ACAGACTCCT GTTTGAAGTA
    TTTGATGAAA ACAGACTGAC ACGGGACGAC TTCCTGGGAC AGGTGGACAT ACCCCTTAGT
    CATCTCCCGA CAGAAGACCC AACCATGGAG CGACCCTACA CATTTAAGGA CTTTCTCCTC
    CGGCCAAGAA GTCATAAATC TCGGGTTAAG GGATTTTTGC GATTGAAAAT GGCCTATATG
    CCCAAAAACG GAGGCCAAGA TGAAGAGAAC AGTGAGCAGC GGGATGAGAT GGAGCCCGGG
    TGGGAAGTCG TTGACTCCAA TGACTCGGCT TCTCAGCACC AGGAGGAGCT GCCCCCGCCT
    CCGCTGCCCC CCGGGTGGGA AGAGAAGGTG GACAACCTGG GCCGCACGTA CTACGTCAAC
    CACAACAACC GGACCACCCA GTGGCACCGG CCCAGCCTCA TGGACGTGTC GTCCGAGTCC
    GACAGCAACA TCCGGCAGAT CAACCAGGAG GCCGCGCATC GCCGCTTCCG CTCGCGCAGA
    CATATCAGCG AGGACCTGGA GCCGGAGCCC AGCGAGGGCG GGGAGGGCCC CGAGCCTTGG
    GAGACCATTT CAGAGGAAGT GAACATGAGC GGAGACTCGC TCAGCCTGGC GCTGCCCCCG
    CCACCGGCCT CGCCCGTGTC CCGGACGAGC CCCCAGGAGC TGTCCGAGGA GCTGAGCAGA
    AGGCTGCAGG TCACTCCCGA CTCCAACGGC GAGCAGTTTG GGTCTTTGAT TCAGAGAGAG
    CCGTCCTCCA GGCTGAGGTC CTGCAGTGTC ACCGACGCAG TCGCGGAGCA GGCTCACCTC
    CCGCCGCCCA GTGCCCCAGC TGGGAGAGCA CGTTCATCAA CTGTCACTGG TGGTGAGGCG
    CCAACGCCAT CAGTGGCCTA TGTACACACC ACGCCGGGCC TGCCCTCGGG CTGGGAAGAG
    AGGAAAGATG CTAAGGGACG CACATACTAT GTCAATCATA ACAATCGAAC CACAACTTGG
    ACTCGGCCTA TCATGCAGCT CGCAGAAGAC GGCGCGTCCG GATCCGCCAC AAACAGTAAC
    AACCACCTAA TCGAGCCTCA GATCCGCCGG CCTCGTAGCC TCAGCTCGCC AACAGTAACT
    TTATCCGCCC CGCTGGAGGG CGCCAAGGAC TCACCCGTAC GTCGGGCTGT GAAAGATACC
    CTTTCCAACC CACAGTCCCC ACAGCCATCA CCTTACAACT CCCCCAAACC ACAACACAAA
    GTCACACAGA GCTTCCTGCC GCCCGGCTGG GAGATGAGGA TAGCGCCCAA CGGCCGGCCC
    TTCTTCATTG ACCACAACAC AAAGACCACG ACGTGGGAAG ATCCACGCCT GAAATTTCCA
    GTACACATGC GGTCAAAGGC ATCCTTAAAC CCCAATGACC TTGGCCCTCT TCCTCCTGGC
    TGGGAAGAAA GGATCCACTT GGATGGCCGG ACGTTTTACA TTGACCACAA TAGCAAAATT
    ACTCAGTGGG AAGACCCAAG ACTGCAGAAC CCAGCTATTA CTGGTCCGGC TGTTCCTTAC
    TCCAGAGAAT TTAAGCAGAA ATATGACTAC TTTAGAAAGA AATTAAAGAA ACCTGCGGAT
    ATTCCAAATA GGTTTGAAAT GAAACTTCAC AGAAATAACA TATTTGAAGA GTCCTATCGG
    AGAATCATGT CTGTGAAAAG ACCAGATGTC CTGAAAGCTA GGCTGTGGAT TGAGTTTGAA
    TCGGAGAAAG GTCTGGACTA CGGGGGCGTG GCCAGAGAGT GGTTCTTCCT ACTGTCCAAG
    GAGATGTTCA ACCCTTACTA CGGCCTCTTC GAGTATTCGG CAACGGACAA CTACACACTT
    CAGATCAATC CCAATTCAGG CCTCTGTAAT GAAGATCATT TGTCCTATTT CACTTTCATT
    GGAAGAGTTG CTGGGCTGGC AGTATTTCAT GGGAAACTTC TAGATGGTTT CTTCATTCGA
    CCGTTTTATA AAATGATGCT GGGAAAGCAG ATAACTCTGA ATGACATGGA ATCTGTGGAT
    AGCGAATATT ACAACTCTTT GAAATGGATC TTAGAAAATG ACCCAACTGA ACTGGACCTT
    ATGTTCTGCA TTGATGAAGA GAACTTTGGG CAGACATATC AAGTGGATCT GAAGCCCAAC
    GGGTCAGAAA TAATGGTAAC GAACGAAAAC AAGAGAGAGT ACATCGACCT CGTCATCCAG
    TGGAGGTTCG TGAACAGGGT CCAGAAGCAG ATGAACGCCT TCCTGGAGGG ATTCACGGAA
    CTGCTCCCTA TTGATTTGAT TAAAATCTTT GATGAAAATG AACTGGAGTT GCTGATGTGT
    GGCCTTGGCG ACGTGGATGT GAACGACTGG CGACAACATT CTATTTACAA GAATGGCTAC
    TGCCCGAACC ACCCCGTGAT CCAGTGGTTC TGGAAGGCTG TGCTGCTGAT GGACGCCGAG
    AAGCGCATCC GGTTGCTGCA GTTTGTCACA GGGACGTCCC GAGTACCTAT GAATGGATTT
    GCCGAGCTTT ATGGTTCCAA TGGTCCTCAG CTGTTTACAA TAGAGCAATG GGGAAGTCCT
    GAAAAACTGC CCAGAGCTCA CACGTGCTTT AATCGCCTTG ACTTACCTCC GTATGAAACC
    TTCGAAGATT TACGAGAGAA GCTCCTCATG GCTGTGGAAA ACGCCCAAGG CTTCGAGGGG
    GTGGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015315640.1
    CDS288..3116
    Translation

    Target ORF information:

    RefSeq Version XM_015460154.2
    Organism Bos taurus(cattle)
    Definition Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015460154.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAACCTTG AGGACAATTA TAGTGATCCA TACGTGAAAC TTTCACTGTA CGTAGCGGAT 
    GAGAATCGGG AACTTGCTTT GGTACAAACA AAAACAATTA AAAAGACGCT GAACCCAAAG
    TGGAATGAAG AATTTTATTT TAGAGTAAAT CCATCTAATC ACAGACTCCT GTTTGAAGTA
    TTTGATGAAA ACAGACTGAC ACGGGACGAC TTCCTGGGAC AGGTGGACAT ACCCCTTAGT
    CATCTCCCGA CAGAAGACCC AACCATGGAG CGACCCTACA CATTTAAGGA CTTTCTCCTC
    CGGCCAAGAA GTCATAAATC TCGGGTTAAG GGATTTTTGC GATTGAAAAT GGCCTATATG
    CCCAAAAACG GAGGCCAAGA TGAAGAGAAC AGTGAGCAGC GGGATGAGAT GGAGCCCGGG
    TGGGAAGTCG TTGACTCCAA TGACTCGGCT TCTCAGCACC AGGAGGAGCT GCCCCCGCCT
    CCGCTGCCCC CCGGGTGGGA AGAGAAGGTG GACAACCTGG GCCGCACGTA CTACGTCAAC
    CACAACAACC GGACCACCCA GTGGCACCGG CCCAGCCTCA TGGACGTGTC GTCCGAGTCC
    GACAGCAACA TCCGGCAGAT CAACCAGGAG GCCGCGCATC GCCGCTTCCG CTCGCGCAGA
    CATATCAGCG AGGACCTGGA GCCGGAGCCC AGCGAGGGCG GGGAGGGCCC CGAGCCTTGG
    GAGACCATTT CAGAGGAAGT GAACATGAGC GGAGACTCGC TCAGCCTGGC GCTGCCCCCG
    CCACCGGCCT CGCCCGTGTC CCGGACGAGC CCCCAGGAGC TGTCCGAGGA GCTGAGCAGA
    AGGCTGCAGG TCACTCCCGA CTCCAACGGC GAGCAGTTTG GGTCTTTGAT TCAGAGAGAG
    CCGTCCTCCA GGCTGAGGTC CTGCAGTGTC ACCGACGCAG TCGCGGAGCA GGCTCACCTC
    CCGCCGCCCA GTGCCCCAGC TGGGAGAGCA CGTTCATCAA CTGTCACTGG TGGTGAGGCG
    CCAACGCCAT CAGTGGCCTA TGTACACACC ACGCCGGGCC TGCCCTCGGG CTGGGAAGAG
    AGGAAAGATG CTAAGGGACG CACATACTAT GTCAATCATA ACAATCGAAC CACAACTTGG
    ACTCGGCCTA TCATGCAGCT CGCAGAAGAC GGCGCGTCCG GATCCGCCAC AAACAGTAAC
    AACCACCTAA TCGAGCCTCA GATCCGCCGG CCTCGTAGCC TCAGCTCGCC AACAGTAACT
    TTATCCGCCC CGCTGGAGGG CGCCAAGGAC TCACCCGTAC GTCGGGCTGT GAAAGATACC
    CTTTCCAACC CACAGTCCCC ACAGCCATCA CCTTACAACT CCCCCAAACC ACAACACAAA
    GTCACACAGA GCTTCCTGCC GCCCGGCTGG GAGATGAGGA TAGCGCCCAA CGGCCGGCCC
    TTCTTCATTG ACCACAACAC AAAGACCACG ACGTGGGAAG ATCCACGCCT GAAATTTCCA
    GTACACATGC GGTCAAAGGC ATCCTTAAAC CCCAATGACC TTGGCCCTCT TCCTCCTGGC
    TGGGAAGAAA GGATCCACTT GGATGGCCGG ACGTTTTACA TTGACCACAA TAGCAAAATT
    ACTCAGTGGG AAGACCCAAG ACTGCAGAAC CCAGCTATTA CTGGTCCGGC TGTTCCTTAC
    TCCAGAGAAT TTAAGCAGAA ATATGACTAC TTTAGAAAGA AATTAAAGAA ACCTGCGGAT
    ATTCCAAATA GGTTTGAAAT GAAACTTCAC AGAAATAACA TATTTGAAGA GTCCTATCGG
    AGAATCATGT CTGTGAAAAG ACCAGATGTC CTGAAAGCTA GGCTGTGGAT TGAGTTTGAA
    TCGGAGAAAG GTCTGGACTA CGGGGGCGTG GCCAGAGAGT GGTTCTTCCT ACTGTCCAAG
    GAGATGTTCA ACCCTTACTA CGGCCTCTTC GAGTATTCGG CAACGGACAA CTACACACTT
    CAGATCAATC CCAATTCAGG CCTCTGTAAT GAAGATCATT TGTCCTATTT CACTTTCATT
    GGAAGAGTTG CTGGGCTGGC AGTATTTCAT GGGAAACTTC TAGATGGTTT CTTCATTCGA
    CCGTTTTATA AAATGATGCT GGGAAAGCAG ATAACTCTGA ATGACATGGA ATCTGTGGAT
    AGCGAATATT ACAACTCTTT GAAATGGATC TTAGAAAATG ACCCAACTGA ACTGGACCTT
    ATGTTCTGCA TTGATGAAGA GAACTTTGGG CAGACATATC AAGTGGATCT GAAGCCCAAC
    GGGTCAGAAA TAATGGTAAC GAACGAAAAC AAGAGAGAGT ACATCGACCT CGTCATCCAG
    TGGAGGTTCG TGAACAGGGT CCAGAAGCAG ATGAACGCCT TCCTGGAGGG ATTCACGGAA
    CTGCTCCCTA TTGATTTGAT TAAAATCTTT GATGAAAATG AACTGGAGTT GCTGATGTGT
    GGCCTTGGCG ACGTGGATGT GAACGACTGG CGACAACATT CTATTTACAA GAATGGCTAC
    TGCCCGAACC ACCCCGTGAT CCAGTGGTTC TGGAAGGCTG TGCTGCTGAT GGACGCCGAG
    AAGCGCATCC GGTTGCTGCA GTTTGTCACA GGGACGTCCC GAGTACCTAT GAATGGATTT
    GCCGAGCTTT ATGGTTCCAA TGGTCCTCAG CTGTTTACAA TAGAGCAATG GGGAAGTCCT
    GAAAAACTGC CCAGAGCTCA CACGTGCTTT AATCGCCTTG ACTTACCTCC GTATGAAACC
    TTCGAAGATT TACGAGAGAA GCTCCTCATG GCTGTGGAAA ACGCCCAAGG CTTCGAGGGG
    GTGGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa47560
    Clone ID Related Accession (Same CDS sequence) XM_010799400.2 , XM_010819011.2 , XM_010819011.3
    Accession Version XM_010799400.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2883bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646636.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010799400.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAACCTTG AGGACAATTA TAGTGATCCA TACGTGAAAC TTTCACTGTA CGTAGCGGAT 
    GAGAATCGGG AACTTGCTTT GGTACAAACA AAAACAATTA AAAAGACGCT GAACCCAAAG
    TGGAATGAAG AATTTTATTT TAGAGTAAAT CCATCTAATC ACAGACTCCT GTTTGAAGTA
    TTTGATGAAA ACAGACTGAC ACGGGACGAC TTCCTGGGAC AGGTGGACAT ACCCCTTAGT
    CATCTCCCGA CAGAAGACCC AACCATGGAG CGACCCTACA CATTTAAGGA CTTTCTCCTC
    CGGCCAAGAA GTCATAAATC TCGGGTTAAG GGATTTTTGC GATTGAAAAT GGCCTATATG
    CCCAAAAACG GAGGCCAAGA TGAAGAGAAC AGTGAGCAGC GGGATGAGAT GGAGCCCGGG
    TGGGAAGTCG TTGACTCCAA TGACTCGGCT TCTCAGCACC AGGAGGAGCT GCCCCCGCCT
    CCGCTGCCCC CCGGGTGGGA AGAGAAGGTG GACAACCTGG GCCGCACGTA CTACGTCAAC
    CACAACAACC GGACCACCCA GTGGCACCGG CCCAGCCTCA TGGACGTGTC GTCCGAGTCC
    GACAGCAACA TCCGGCAGAT CAACCAGGAG GCCGCGCATC GCCGCTTCCG CTCGCGCAGA
    CATATCAGCG AGGACCTGGA GCCGGAGCCC AGCGAGGGCG GGGAGGGCCC CGAGCCTTGG
    GAGACCATTT CAGAGGAAGT GAACATGAGC GGAGACTCGC TCAGCCTGGC GCTGCCCCCG
    CCACCGGCCT CGCCCGTGTC CCGGACGAGC CCCCAGGAGC TGTCCGAGGA GCTGAGCAGA
    AGGCTGCAGG TCACTCCCGA CTCCAACGGC GAGCAGTTTG GGTCTTTGAT TCAGAGAGAG
    CCGTCCTCCA GGCTGAGGTC CTGCAGTGTC ACCGACGCAG TCGCGGAGCA GGCTCACCTC
    CCGCCGCCCA GTGCCCCAGC TGGGAGAGCA CGTTCATCAA CTGTCACTGG TGGTGAGGCG
    CCAACGCCAT CAGTGGCCTA TGTACACACC ACGCCGGGCC TGCCCTCGGG CTGGGAAGAG
    AGGAAAGATG CTAAGGGACG CACATACTAT GTCAATCATA ACAATCGAAC CACAACTTGG
    ACTCGGCCTA TCATGCAGCT CGCAGAAGAC GGCGCGTCCG GATCCGCCAC AAACAGTAAC
    AACCACCTAA TCGAGCCTCA GATCCGCCGG CCTCGTAGCC TCAGCTCGCC AACAGTAACT
    TTATCCGCCC CGCTGGAGGG CGCCAAGGAC TCACCCGTAC GTCGGGCTGT GAAAGATACC
    CTTTCCAACC CACAGTCCCC ACAGCCATCA CCTTACAACT CCCCCAAACC ACAACACAAA
    GTCACACAGA GCTTCCTGCC GCCCGGCTGG GAGATGAGGA TAGCGCCCAA CGGCCGGCCC
    TTCTTCATTG ACCACAACAC AAAGACCACG ACGTGGGAAG ATCCACGCCT GAAATTTCCA
    GTACACATGC GGTCAAAGGC ATCCTTAAAC CCCAATGACC TTGGCCCTCT TCCTCCTGGC
    TGGGAAGAAA GGATCCACTT GGATGGCCGG ACGTTTTACA TTGACCACAG CAGCCAGGTG
    TTATGTGGCG AGGACGATGC CAGAGACTCA GTGCCCTCGT ACGATAGCAA AATTACTCAG
    TGGGAAGACC CAAGACTGCA GAACCCAGCT ATTACTGGTC CGGCTGTTCC TTACTCCAGA
    GAATTTAAGC AGAAATATGA CTACTTTAGA AAGAAATTAA AGAAACCTGC GGATATTCCA
    AATAGGTTTG AAATGAAACT TCACAGAAAT AACATATTTG AAGAGTCCTA TCGGAGAATC
    ATGTCTGTGA AAAGACCAGA TGTCCTGAAA GCTAGGCTGT GGATTGAGTT TGAATCGGAG
    AAAGGTCTGG ACTACGGGGG CGTGGCCAGA GAGTGGTTCT TCCTACTGTC CAAGGAGATG
    TTCAACCCTT ACTACGGCCT CTTCGAGTAT TCGGCAACGG ACAACTACAC ACTTCAGATC
    AATCCCAATT CAGGCCTCTG TAATGAAGAT CATTTGTCCT ATTTCACTTT CATTGGAAGA
    GTTGCTGGGC TGGCAGTATT TCATGGGAAA CTTCTAGATG GTTTCTTCAT TCGACCGTTT
    TATAAAATGA TGCTGGGAAA GCAGATAACT CTGAATGACA TGGAATCTGT GGATAGCGAA
    TATTACAACT CTTTGAAATG GATCTTAGAA AATGACCCAA CTGAACTGGA CCTTATGTTC
    TGCATTGATG AAGAGAACTT TGGGCAGACA TATCAAGTGG ATCTGAAGCC CAACGGGTCA
    GAAATAATGG TAACGAACGA AAACAAGAGA GAGTACATCG ACCTCGTCAT CCAGTGGAGG
    TTCGTGAACA GGGTCCAGAA GCAGATGAAC GCCTTCCTGG AGGGATTCAC GGAACTGCTC
    CCTATTGATT TGATTAAAAT CTTTGATGAA AATGAACTGG AGTTGCTGAT GTGTGGCCTT
    GGCGACGTGG ATGTGAACGA CTGGCGACAA CATTCTATTT ACAAGAATGG CTACTGCCCG
    AACCACCCCG TGATCCAGTG GTTCTGGAAG GCTGTGCTGC TGATGGACGC CGAGAAGCGC
    ATCCGGTTGC TGCAGTTTGT CACAGGGACG TCCCGAGTAC CTATGAATGG ATTTGCCGAG
    CTTTATGGTT CCAATGGTCC TCAGCTGTTT ACAATAGAGC AATGGGGAAG TCCTGAAAAA
    CTGCCCAGAG CTCACACGTG CTTTAATCGC CTTGACTTAC CTCCGTATGA AACCTTCGAA
    GATTTACGAG AGAAGCTCCT CATGGCTGTG GAAAACGCCC AAGGCTTCGA GGGGGTGGAT
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010797702.1
    CDS228..3110
    Translation

    Target ORF information:

    RefSeq Version XM_010799400.2
    Organism Bos taurus(cattle)
    Definition Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010799400.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAACCTTG AGGACAATTA TAGTGATCCA TACGTGAAAC TTTCACTGTA CGTAGCGGAT 
    GAGAATCGGG AACTTGCTTT GGTACAAACA AAAACAATTA AAAAGACGCT GAACCCAAAG
    TGGAATGAAG AATTTTATTT TAGAGTAAAT CCATCTAATC ACAGACTCCT GTTTGAAGTA
    TTTGATGAAA ACAGACTGAC ACGGGACGAC TTCCTGGGAC AGGTGGACAT ACCCCTTAGT
    CATCTCCCGA CAGAAGACCC AACCATGGAG CGACCCTACA CATTTAAGGA CTTTCTCCTC
    CGGCCAAGAA GTCATAAATC TCGGGTTAAG GGATTTTTGC GATTGAAAAT GGCCTATATG
    CCCAAAAACG GAGGCCAAGA TGAAGAGAAC AGTGAGCAGC GGGATGAGAT GGAGCCCGGG
    TGGGAAGTCG TTGACTCCAA TGACTCGGCT TCTCAGCACC AGGAGGAGCT GCCCCCGCCT
    CCGCTGCCCC CCGGGTGGGA AGAGAAGGTG GACAACCTGG GCCGCACGTA CTACGTCAAC
    CACAACAACC GGACCACCCA GTGGCACCGG CCCAGCCTCA TGGACGTGTC GTCCGAGTCC
    GACAGCAACA TCCGGCAGAT CAACCAGGAG GCCGCGCATC GCCGCTTCCG CTCGCGCAGA
    CATATCAGCG AGGACCTGGA GCCGGAGCCC AGCGAGGGCG GGGAGGGCCC CGAGCCTTGG
    GAGACCATTT CAGAGGAAGT GAACATGAGC GGAGACTCGC TCAGCCTGGC GCTGCCCCCG
    CCACCGGCCT CGCCCGTGTC CCGGACGAGC CCCCAGGAGC TGTCCGAGGA GCTGAGCAGA
    AGGCTGCAGG TCACTCCCGA CTCCAACGGC GAGCAGTTTG GGTCTTTGAT TCAGAGAGAG
    CCGTCCTCCA GGCTGAGGTC CTGCAGTGTC ACCGACGCAG TCGCGGAGCA GGCTCACCTC
    CCGCCGCCCA GTGCCCCAGC TGGGAGAGCA CGTTCATCAA CTGTCACTGG TGGTGAGGCG
    CCAACGCCAT CAGTGGCCTA TGTACACACC ACGCCGGGCC TGCCCTCGGG CTGGGAAGAG
    AGGAAAGATG CTAAGGGACG CACATACTAT GTCAATCATA ACAATCGAAC CACAACTTGG
    ACTCGGCCTA TCATGCAGCT CGCAGAAGAC GGCGCGTCCG GATCCGCCAC AAACAGTAAC
    AACCACCTAA TCGAGCCTCA GATCCGCCGG CCTCGTAGCC TCAGCTCGCC AACAGTAACT
    TTATCCGCCC CGCTGGAGGG CGCCAAGGAC TCACCCGTAC GTCGGGCTGT GAAAGATACC
    CTTTCCAACC CACAGTCCCC ACAGCCATCA CCTTACAACT CCCCCAAACC ACAACACAAA
    GTCACACAGA GCTTCCTGCC GCCCGGCTGG GAGATGAGGA TAGCGCCCAA CGGCCGGCCC
    TTCTTCATTG ACCACAACAC AAAGACCACG ACGTGGGAAG ATCCACGCCT GAAATTTCCA
    GTACACATGC GGTCAAAGGC ATCCTTAAAC CCCAATGACC TTGGCCCTCT TCCTCCTGGC
    TGGGAAGAAA GGATCCACTT GGATGGCCGG ACGTTTTACA TTGACCACAG CAGCCAGGTG
    TTATGTGGCG AGGACGATGC CAGAGACTCA GTGCCCTCGT ACGATAGCAA AATTACTCAG
    TGGGAAGACC CAAGACTGCA GAACCCAGCT ATTACTGGTC CGGCTGTTCC TTACTCCAGA
    GAATTTAAGC AGAAATATGA CTACTTTAGA AAGAAATTAA AGAAACCTGC GGATATTCCA
    AATAGGTTTG AAATGAAACT TCACAGAAAT AACATATTTG AAGAGTCCTA TCGGAGAATC
    ATGTCTGTGA AAAGACCAGA TGTCCTGAAA GCTAGGCTGT GGATTGAGTT TGAATCGGAG
    AAAGGTCTGG ACTACGGGGG CGTGGCCAGA GAGTGGTTCT TCCTACTGTC CAAGGAGATG
    TTCAACCCTT ACTACGGCCT CTTCGAGTAT TCGGCAACGG ACAACTACAC ACTTCAGATC
    AATCCCAATT CAGGCCTCTG TAATGAAGAT CATTTGTCCT ATTTCACTTT CATTGGAAGA
    GTTGCTGGGC TGGCAGTATT TCATGGGAAA CTTCTAGATG GTTTCTTCAT TCGACCGTTT
    TATAAAATGA TGCTGGGAAA GCAGATAACT CTGAATGACA TGGAATCTGT GGATAGCGAA
    TATTACAACT CTTTGAAATG GATCTTAGAA AATGACCCAA CTGAACTGGA CCTTATGTTC
    TGCATTGATG AAGAGAACTT TGGGCAGACA TATCAAGTGG ATCTGAAGCC CAACGGGTCA
    GAAATAATGG TAACGAACGA AAACAAGAGA GAGTACATCG ACCTCGTCAT CCAGTGGAGG
    TTCGTGAACA GGGTCCAGAA GCAGATGAAC GCCTTCCTGG AGGGATTCAC GGAACTGCTC
    CCTATTGATT TGATTAAAAT CTTTGATGAA AATGAACTGG AGTTGCTGAT GTGTGGCCTT
    GGCGACGTGG ATGTGAACGA CTGGCGACAA CATTCTATTT ACAAGAATGG CTACTGCCCG
    AACCACCCCG TGATCCAGTG GTTCTGGAAG GCTGTGCTGC TGATGGACGC CGAGAAGCGC
    ATCCGGTTGC TGCAGTTTGT CACAGGGACG TCCCGAGTAC CTATGAATGG ATTTGCCGAG
    CTTTATGGTT CCAATGGTCC TCAGCTGTTT ACAATAGAGC AATGGGGAAG TCCTGAAAAA
    CTGCCCAGAG CTCACACGTG CTTTAATCGC CTTGACTTAC CTCCGTATGA AACCTTCGAA
    GATTTACGAG AGAAGCTCCT CATGGCTGTG GAAAACGCCC AAGGCTTCGA GGGGGTGGAT
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa47560
    Clone ID Related Accession (Same CDS sequence) XM_010799400.2 , XM_010819011.2 , XM_010819011.3
    Accession Version XM_010819011.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2883bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104569.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010819011.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAACCTTG AGGACAATTA TAGTGATCCA TACGTGAAAC TTTCACTGTA CGTAGCGGAT 
    GAGAATCGGG AACTTGCTTT GGTACAAACA AAAACAATTA AAAAGACGCT GAACCCAAAG
    TGGAATGAAG AATTTTATTT TAGAGTAAAT CCATCTAATC ACAGACTCCT GTTTGAAGTA
    TTTGATGAAA ACAGACTGAC ACGGGACGAC TTCCTGGGAC AGGTGGACAT ACCCCTTAGT
    CATCTCCCGA CAGAAGACCC AACCATGGAG CGACCCTACA CATTTAAGGA CTTTCTCCTC
    CGGCCAAGAA GTCATAAATC TCGGGTTAAG GGATTTTTGC GATTGAAAAT GGCCTATATG
    CCCAAAAACG GAGGCCAAGA TGAAGAGAAC AGTGAGCAGC GGGATGAGAT GGAGCCCGGG
    TGGGAAGTCG TTGACTCCAA TGACTCGGCT TCTCAGCACC AGGAGGAGCT GCCCCCGCCT
    CCGCTGCCCC CCGGGTGGGA AGAGAAGGTG GACAACCTGG GCCGCACGTA CTACGTCAAC
    CACAACAACC GGACCACCCA GTGGCACCGG CCCAGCCTCA TGGACGTGTC GTCCGAGTCC
    GACAGCAACA TCCGGCAGAT CAACCAGGAG GCCGCGCATC GCCGCTTCCG CTCGCGCAGA
    CATATCAGCG AGGACCTGGA GCCGGAGCCC AGCGAGGGCG GGGAGGGCCC CGAGCCTTGG
    GAGACCATTT CAGAGGAAGT GAACATGAGC GGAGACTCGC TCAGCCTGGC GCTGCCCCCG
    CCACCGGCCT CGCCCGTGTC CCGGACGAGC CCCCAGGAGC TGTCCGAGGA GCTGAGCAGA
    AGGCTGCAGG TCACTCCCGA CTCCAACGGC GAGCAGTTTG GGTCTTTGAT TCAGAGAGAG
    CCGTCCTCCA GGCTGAGGTC CTGCAGTGTC ACCGACGCAG TCGCGGAGCA GGCTCACCTC
    CCGCCGCCCA GTGCCCCAGC TGGGAGAGCA CGTTCATCAA CTGTCACTGG TGGTGAGGCG
    CCAACGCCAT CAGTGGCCTA TGTACACACC ACGCCGGGCC TGCCCTCGGG CTGGGAAGAG
    AGGAAAGATG CTAAGGGACG CACATACTAT GTCAATCATA ACAATCGAAC CACAACTTGG
    ACTCGGCCTA TCATGCAGCT CGCAGAAGAC GGCGCGTCCG GATCCGCCAC AAACAGTAAC
    AACCACCTAA TCGAGCCTCA GATCCGCCGG CCTCGTAGCC TCAGCTCGCC AACAGTAACT
    TTATCCGCCC CGCTGGAGGG CGCCAAGGAC TCACCCGTAC GTCGGGCTGT GAAAGATACC
    CTTTCCAACC CACAGTCCCC ACAGCCATCA CCTTACAACT CCCCCAAACC ACAACACAAA
    GTCACACAGA GCTTCCTGCC GCCCGGCTGG GAGATGAGGA TAGCGCCCAA CGGCCGGCCC
    TTCTTCATTG ACCACAACAC AAAGACCACG ACGTGGGAAG ATCCACGCCT GAAATTTCCA
    GTACACATGC GGTCAAAGGC ATCCTTAAAC CCCAATGACC TTGGCCCTCT TCCTCCTGGC
    TGGGAAGAAA GGATCCACTT GGATGGCCGG ACGTTTTACA TTGACCACAG CAGCCAGGTG
    TTATGTGGCG AGGACGATGC CAGAGACTCA GTGCCCTCGT ACGATAGCAA AATTACTCAG
    TGGGAAGACC CAAGACTGCA GAACCCAGCT ATTACTGGTC CGGCTGTTCC TTACTCCAGA
    GAATTTAAGC AGAAATATGA CTACTTTAGA AAGAAATTAA AGAAACCTGC GGATATTCCA
    AATAGGTTTG AAATGAAACT TCACAGAAAT AACATATTTG AAGAGTCCTA TCGGAGAATC
    ATGTCTGTGA AAAGACCAGA TGTCCTGAAA GCTAGGCTGT GGATTGAGTT TGAATCGGAG
    AAAGGTCTGG ACTACGGGGG CGTGGCCAGA GAGTGGTTCT TCCTACTGTC CAAGGAGATG
    TTCAACCCTT ACTACGGCCT CTTCGAGTAT TCGGCAACGG ACAACTACAC ACTTCAGATC
    AATCCCAATT CAGGCCTCTG TAATGAAGAT CATTTGTCCT ATTTCACTTT CATTGGAAGA
    GTTGCTGGGC TGGCAGTATT TCATGGGAAA CTTCTAGATG GTTTCTTCAT TCGACCGTTT
    TATAAAATGA TGCTGGGAAA GCAGATAACT CTGAATGACA TGGAATCTGT GGATAGCGAA
    TATTACAACT CTTTGAAATG GATCTTAGAA AATGACCCAA CTGAACTGGA CCTTATGTTC
    TGCATTGATG AAGAGAACTT TGGGCAGACA TATCAAGTGG ATCTGAAGCC CAACGGGTCA
    GAAATAATGG TAACGAACGA AAACAAGAGA GAGTACATCG ACCTCGTCAT CCAGTGGAGG
    TTCGTGAACA GGGTCCAGAA GCAGATGAAC GCCTTCCTGG AGGGATTCAC GGAACTGCTC
    CCTATTGATT TGATTAAAAT CTTTGATGAA AATGAACTGG AGTTGCTGAT GTGTGGCCTT
    GGCGACGTGG ATGTGAACGA CTGGCGACAA CATTCTATTT ACAAGAATGG CTACTGCCCG
    AACCACCCCG TGATCCAGTG GTTCTGGAAG GCTGTGCTGC TGATGGACGC CGAGAAGCGC
    ATCCGGTTGC TGCAGTTTGT CACAGGGACG TCCCGAGTAC CTATGAATGG ATTTGCCGAG
    CTTTATGGTT CCAATGGTCC TCAGCTGTTT ACAATAGAGC AATGGGGAAG TCCTGAAAAA
    CTGCCCAGAG CTCACACGTG CTTTAATCGC CTTGACTTAC CTCCGTATGA AACCTTCGAA
    GATTTACGAG AGAAGCTCCT CATGGCTGTG GAAAACGCCC AAGGCTTCGA GGGGGTGGAT
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010817313.1
    CDS228..3110
    Translation

    Target ORF information:

    RefSeq Version XM_010819011.2
    Organism Bos taurus(cattle)
    Definition Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010819011.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAACCTTG AGGACAATTA TAGTGATCCA TACGTGAAAC TTTCACTGTA CGTAGCGGAT 
    GAGAATCGGG AACTTGCTTT GGTACAAACA AAAACAATTA AAAAGACGCT GAACCCAAAG
    TGGAATGAAG AATTTTATTT TAGAGTAAAT CCATCTAATC ACAGACTCCT GTTTGAAGTA
    TTTGATGAAA ACAGACTGAC ACGGGACGAC TTCCTGGGAC AGGTGGACAT ACCCCTTAGT
    CATCTCCCGA CAGAAGACCC AACCATGGAG CGACCCTACA CATTTAAGGA CTTTCTCCTC
    CGGCCAAGAA GTCATAAATC TCGGGTTAAG GGATTTTTGC GATTGAAAAT GGCCTATATG
    CCCAAAAACG GAGGCCAAGA TGAAGAGAAC AGTGAGCAGC GGGATGAGAT GGAGCCCGGG
    TGGGAAGTCG TTGACTCCAA TGACTCGGCT TCTCAGCACC AGGAGGAGCT GCCCCCGCCT
    CCGCTGCCCC CCGGGTGGGA AGAGAAGGTG GACAACCTGG GCCGCACGTA CTACGTCAAC
    CACAACAACC GGACCACCCA GTGGCACCGG CCCAGCCTCA TGGACGTGTC GTCCGAGTCC
    GACAGCAACA TCCGGCAGAT CAACCAGGAG GCCGCGCATC GCCGCTTCCG CTCGCGCAGA
    CATATCAGCG AGGACCTGGA GCCGGAGCCC AGCGAGGGCG GGGAGGGCCC CGAGCCTTGG
    GAGACCATTT CAGAGGAAGT GAACATGAGC GGAGACTCGC TCAGCCTGGC GCTGCCCCCG
    CCACCGGCCT CGCCCGTGTC CCGGACGAGC CCCCAGGAGC TGTCCGAGGA GCTGAGCAGA
    AGGCTGCAGG TCACTCCCGA CTCCAACGGC GAGCAGTTTG GGTCTTTGAT TCAGAGAGAG
    CCGTCCTCCA GGCTGAGGTC CTGCAGTGTC ACCGACGCAG TCGCGGAGCA GGCTCACCTC
    CCGCCGCCCA GTGCCCCAGC TGGGAGAGCA CGTTCATCAA CTGTCACTGG TGGTGAGGCG
    CCAACGCCAT CAGTGGCCTA TGTACACACC ACGCCGGGCC TGCCCTCGGG CTGGGAAGAG
    AGGAAAGATG CTAAGGGACG CACATACTAT GTCAATCATA ACAATCGAAC CACAACTTGG
    ACTCGGCCTA TCATGCAGCT CGCAGAAGAC GGCGCGTCCG GATCCGCCAC AAACAGTAAC
    AACCACCTAA TCGAGCCTCA GATCCGCCGG CCTCGTAGCC TCAGCTCGCC AACAGTAACT
    TTATCCGCCC CGCTGGAGGG CGCCAAGGAC TCACCCGTAC GTCGGGCTGT GAAAGATACC
    CTTTCCAACC CACAGTCCCC ACAGCCATCA CCTTACAACT CCCCCAAACC ACAACACAAA
    GTCACACAGA GCTTCCTGCC GCCCGGCTGG GAGATGAGGA TAGCGCCCAA CGGCCGGCCC
    TTCTTCATTG ACCACAACAC AAAGACCACG ACGTGGGAAG ATCCACGCCT GAAATTTCCA
    GTACACATGC GGTCAAAGGC ATCCTTAAAC CCCAATGACC TTGGCCCTCT TCCTCCTGGC
    TGGGAAGAAA GGATCCACTT GGATGGCCGG ACGTTTTACA TTGACCACAG CAGCCAGGTG
    TTATGTGGCG AGGACGATGC CAGAGACTCA GTGCCCTCGT ACGATAGCAA AATTACTCAG
    TGGGAAGACC CAAGACTGCA GAACCCAGCT ATTACTGGTC CGGCTGTTCC TTACTCCAGA
    GAATTTAAGC AGAAATATGA CTACTTTAGA AAGAAATTAA AGAAACCTGC GGATATTCCA
    AATAGGTTTG AAATGAAACT TCACAGAAAT AACATATTTG AAGAGTCCTA TCGGAGAATC
    ATGTCTGTGA AAAGACCAGA TGTCCTGAAA GCTAGGCTGT GGATTGAGTT TGAATCGGAG
    AAAGGTCTGG ACTACGGGGG CGTGGCCAGA GAGTGGTTCT TCCTACTGTC CAAGGAGATG
    TTCAACCCTT ACTACGGCCT CTTCGAGTAT TCGGCAACGG ACAACTACAC ACTTCAGATC
    AATCCCAATT CAGGCCTCTG TAATGAAGAT CATTTGTCCT ATTTCACTTT CATTGGAAGA
    GTTGCTGGGC TGGCAGTATT TCATGGGAAA CTTCTAGATG GTTTCTTCAT TCGACCGTTT
    TATAAAATGA TGCTGGGAAA GCAGATAACT CTGAATGACA TGGAATCTGT GGATAGCGAA
    TATTACAACT CTTTGAAATG GATCTTAGAA AATGACCCAA CTGAACTGGA CCTTATGTTC
    TGCATTGATG AAGAGAACTT TGGGCAGACA TATCAAGTGG ATCTGAAGCC CAACGGGTCA
    GAAATAATGG TAACGAACGA AAACAAGAGA GAGTACATCG ACCTCGTCAT CCAGTGGAGG
    TTCGTGAACA GGGTCCAGAA GCAGATGAAC GCCTTCCTGG AGGGATTCAC GGAACTGCTC
    CCTATTGATT TGATTAAAAT CTTTGATGAA AATGAACTGG AGTTGCTGAT GTGTGGCCTT
    GGCGACGTGG ATGTGAACGA CTGGCGACAA CATTCTATTT ACAAGAATGG CTACTGCCCG
    AACCACCCCG TGATCCAGTG GTTCTGGAAG GCTGTGCTGC TGATGGACGC CGAGAAGCGC
    ATCCGGTTGC TGCAGTTTGT CACAGGGACG TCCCGAGTAC CTATGAATGG ATTTGCCGAG
    CTTTATGGTT CCAATGGTCC TCAGCTGTTT ACAATAGAGC AATGGGGAAG TCCTGAAAAA
    CTGCCCAGAG CTCACACGTG CTTTAATCGC CTTGACTTAC CTCCGTATGA AACCTTCGAA
    GATTTACGAG AGAAGCTCCT CATGGCTGTG GAAAACGCCC AAGGCTTCGA GGGGGTGGAT
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa47560
    Clone ID Related Accession (Same CDS sequence) XM_010799400.2 , XM_010819011.2 , XM_010819011.3
    Accession Version XM_010819011.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2883bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010819011.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAACCTTG AGGACAATTA TAGTGATCCA TACGTGAAAC TTTCACTGTA CGTAGCGGAT 
    GAGAATCGGG AACTTGCTTT GGTACAAACA AAAACAATTA AAAAGACGCT GAACCCAAAG
    TGGAATGAAG AATTTTATTT TAGAGTAAAT CCATCTAATC ACAGACTCCT GTTTGAAGTA
    TTTGATGAAA ACAGACTGAC ACGGGACGAC TTCCTGGGAC AGGTGGACAT ACCCCTTAGT
    CATCTCCCGA CAGAAGACCC AACCATGGAG CGACCCTACA CATTTAAGGA CTTTCTCCTC
    CGGCCAAGAA GTCATAAATC TCGGGTTAAG GGATTTTTGC GATTGAAAAT GGCCTATATG
    CCCAAAAACG GAGGCCAAGA TGAAGAGAAC AGTGAGCAGC GGGATGAGAT GGAGCCCGGG
    TGGGAAGTCG TTGACTCCAA TGACTCGGCT TCTCAGCACC AGGAGGAGCT GCCCCCGCCT
    CCGCTGCCCC CCGGGTGGGA AGAGAAGGTG GACAACCTGG GCCGCACGTA CTACGTCAAC
    CACAACAACC GGACCACCCA GTGGCACCGG CCCAGCCTCA TGGACGTGTC GTCCGAGTCC
    GACAGCAACA TCCGGCAGAT CAACCAGGAG GCCGCGCATC GCCGCTTCCG CTCGCGCAGA
    CATATCAGCG AGGACCTGGA GCCGGAGCCC AGCGAGGGCG GGGAGGGCCC CGAGCCTTGG
    GAGACCATTT CAGAGGAAGT GAACATGAGC GGAGACTCGC TCAGCCTGGC GCTGCCCCCG
    CCACCGGCCT CGCCCGTGTC CCGGACGAGC CCCCAGGAGC TGTCCGAGGA GCTGAGCAGA
    AGGCTGCAGG TCACTCCCGA CTCCAACGGC GAGCAGTTTG GGTCTTTGAT TCAGAGAGAG
    CCGTCCTCCA GGCTGAGGTC CTGCAGTGTC ACCGACGCAG TCGCGGAGCA GGCTCACCTC
    CCGCCGCCCA GTGCCCCAGC TGGGAGAGCA CGTTCATCAA CTGTCACTGG TGGTGAGGCG
    CCAACGCCAT CAGTGGCCTA TGTACACACC ACGCCGGGCC TGCCCTCGGG CTGGGAAGAG
    AGGAAAGATG CTAAGGGACG CACATACTAT GTCAATCATA ACAATCGAAC CACAACTTGG
    ACTCGGCCTA TCATGCAGCT CGCAGAAGAC GGCGCGTCCG GATCCGCCAC AAACAGTAAC
    AACCACCTAA TCGAGCCTCA GATCCGCCGG CCTCGTAGCC TCAGCTCGCC AACAGTAACT
    TTATCCGCCC CGCTGGAGGG CGCCAAGGAC TCACCCGTAC GTCGGGCTGT GAAAGATACC
    CTTTCCAACC CACAGTCCCC ACAGCCATCA CCTTACAACT CCCCCAAACC ACAACACAAA
    GTCACACAGA GCTTCCTGCC GCCCGGCTGG GAGATGAGGA TAGCGCCCAA CGGCCGGCCC
    TTCTTCATTG ACCACAACAC AAAGACCACG ACGTGGGAAG ATCCACGCCT GAAATTTCCA
    GTACACATGC GGTCAAAGGC ATCCTTAAAC CCCAATGACC TTGGCCCTCT TCCTCCTGGC
    TGGGAAGAAA GGATCCACTT GGATGGCCGG ACGTTTTACA TTGACCACAG CAGCCAGGTG
    TTATGTGGCG AGGACGATGC CAGAGACTCA GTGCCCTCGT ACGATAGCAA AATTACTCAG
    TGGGAAGACC CAAGACTGCA GAACCCAGCT ATTACTGGTC CGGCTGTTCC TTACTCCAGA
    GAATTTAAGC AGAAATATGA CTACTTTAGA AAGAAATTAA AGAAACCTGC GGATATTCCA
    AATAGGTTTG AAATGAAACT TCACAGAAAT AACATATTTG AAGAGTCCTA TCGGAGAATC
    ATGTCTGTGA AAAGACCAGA TGTCCTGAAA GCTAGGCTGT GGATTGAGTT TGAATCGGAG
    AAAGGTCTGG ACTACGGGGG CGTGGCCAGA GAGTGGTTCT TCCTACTGTC CAAGGAGATG
    TTCAACCCTT ACTACGGCCT CTTCGAGTAT TCGGCAACGG ACAACTACAC ACTTCAGATC
    AATCCCAATT CAGGCCTCTG TAATGAAGAT CATTTGTCCT ATTTCACTTT CATTGGAAGA
    GTTGCTGGGC TGGCAGTATT TCATGGGAAA CTTCTAGATG GTTTCTTCAT TCGACCGTTT
    TATAAAATGA TGCTGGGAAA GCAGATAACT CTGAATGACA TGGAATCTGT GGATAGCGAA
    TATTACAACT CTTTGAAATG GATCTTAGAA AATGACCCAA CTGAACTGGA CCTTATGTTC
    TGCATTGATG AAGAGAACTT TGGGCAGACA TATCAAGTGG ATCTGAAGCC CAACGGGTCA
    GAAATAATGG TAACGAACGA AAACAAGAGA GAGTACATCG ACCTCGTCAT CCAGTGGAGG
    TTCGTGAACA GGGTCCAGAA GCAGATGAAC GCCTTCCTGG AGGGATTCAC GGAACTGCTC
    CCTATTGATT TGATTAAAAT CTTTGATGAA AATGAACTGG AGTTGCTGAT GTGTGGCCTT
    GGCGACGTGG ATGTGAACGA CTGGCGACAA CATTCTATTT ACAAGAATGG CTACTGCCCG
    AACCACCCCG TGATCCAGTG GTTCTGGAAG GCTGTGCTGC TGATGGACGC CGAGAAGCGC
    ATCCGGTTGC TGCAGTTTGT CACAGGGACG TCCCGAGTAC CTATGAATGG ATTTGCCGAG
    CTTTATGGTT CCAATGGTCC TCAGCTGTTT ACAATAGAGC AATGGGGAAG TCCTGAAAAA
    CTGCCCAGAG CTCACACGTG CTTTAATCGC CTTGACTTAC CTCCGTATGA AACCTTCGAA
    GATTTACGAG AGAAGCTCCT CATGGCTGTG GAAAACGCCC AAGGCTTCGA GGGGGTGGAT
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010817313.1
    CDS227..3109
    Translation

    Target ORF information:

    RefSeq Version XM_010819011.3
    Organism Bos taurus(cattle)
    Definition Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010819011.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAACCTTG AGGACAATTA TAGTGATCCA TACGTGAAAC TTTCACTGTA CGTAGCGGAT 
    GAGAATCGGG AACTTGCTTT GGTACAAACA AAAACAATTA AAAAGACGCT GAACCCAAAG
    TGGAATGAAG AATTTTATTT TAGAGTAAAT CCATCTAATC ACAGACTCCT GTTTGAAGTA
    TTTGATGAAA ACAGACTGAC ACGGGACGAC TTCCTGGGAC AGGTGGACAT ACCCCTTAGT
    CATCTCCCGA CAGAAGACCC AACCATGGAG CGACCCTACA CATTTAAGGA CTTTCTCCTC
    CGGCCAAGAA GTCATAAATC TCGGGTTAAG GGATTTTTGC GATTGAAAAT GGCCTATATG
    CCCAAAAACG GAGGCCAAGA TGAAGAGAAC AGTGAGCAGC GGGATGAGAT GGAGCCCGGG
    TGGGAAGTCG TTGACTCCAA TGACTCGGCT TCTCAGCACC AGGAGGAGCT GCCCCCGCCT
    CCGCTGCCCC CCGGGTGGGA AGAGAAGGTG GACAACCTGG GCCGCACGTA CTACGTCAAC
    CACAACAACC GGACCACCCA GTGGCACCGG CCCAGCCTCA TGGACGTGTC GTCCGAGTCC
    GACAGCAACA TCCGGCAGAT CAACCAGGAG GCCGCGCATC GCCGCTTCCG CTCGCGCAGA
    CATATCAGCG AGGACCTGGA GCCGGAGCCC AGCGAGGGCG GGGAGGGCCC CGAGCCTTGG
    GAGACCATTT CAGAGGAAGT GAACATGAGC GGAGACTCGC TCAGCCTGGC GCTGCCCCCG
    CCACCGGCCT CGCCCGTGTC CCGGACGAGC CCCCAGGAGC TGTCCGAGGA GCTGAGCAGA
    AGGCTGCAGG TCACTCCCGA CTCCAACGGC GAGCAGTTTG GGTCTTTGAT TCAGAGAGAG
    CCGTCCTCCA GGCTGAGGTC CTGCAGTGTC ACCGACGCAG TCGCGGAGCA GGCTCACCTC
    CCGCCGCCCA GTGCCCCAGC TGGGAGAGCA CGTTCATCAA CTGTCACTGG TGGTGAGGCG
    CCAACGCCAT CAGTGGCCTA TGTACACACC ACGCCGGGCC TGCCCTCGGG CTGGGAAGAG
    AGGAAAGATG CTAAGGGACG CACATACTAT GTCAATCATA ACAATCGAAC CACAACTTGG
    ACTCGGCCTA TCATGCAGCT CGCAGAAGAC GGCGCGTCCG GATCCGCCAC AAACAGTAAC
    AACCACCTAA TCGAGCCTCA GATCCGCCGG CCTCGTAGCC TCAGCTCGCC AACAGTAACT
    TTATCCGCCC CGCTGGAGGG CGCCAAGGAC TCACCCGTAC GTCGGGCTGT GAAAGATACC
    CTTTCCAACC CACAGTCCCC ACAGCCATCA CCTTACAACT CCCCCAAACC ACAACACAAA
    GTCACACAGA GCTTCCTGCC GCCCGGCTGG GAGATGAGGA TAGCGCCCAA CGGCCGGCCC
    TTCTTCATTG ACCACAACAC AAAGACCACG ACGTGGGAAG ATCCACGCCT GAAATTTCCA
    GTACACATGC GGTCAAAGGC ATCCTTAAAC CCCAATGACC TTGGCCCTCT TCCTCCTGGC
    TGGGAAGAAA GGATCCACTT GGATGGCCGG ACGTTTTACA TTGACCACAG CAGCCAGGTG
    TTATGTGGCG AGGACGATGC CAGAGACTCA GTGCCCTCGT ACGATAGCAA AATTACTCAG
    TGGGAAGACC CAAGACTGCA GAACCCAGCT ATTACTGGTC CGGCTGTTCC TTACTCCAGA
    GAATTTAAGC AGAAATATGA CTACTTTAGA AAGAAATTAA AGAAACCTGC GGATATTCCA
    AATAGGTTTG AAATGAAACT TCACAGAAAT AACATATTTG AAGAGTCCTA TCGGAGAATC
    ATGTCTGTGA AAAGACCAGA TGTCCTGAAA GCTAGGCTGT GGATTGAGTT TGAATCGGAG
    AAAGGTCTGG ACTACGGGGG CGTGGCCAGA GAGTGGTTCT TCCTACTGTC CAAGGAGATG
    TTCAACCCTT ACTACGGCCT CTTCGAGTAT TCGGCAACGG ACAACTACAC ACTTCAGATC
    AATCCCAATT CAGGCCTCTG TAATGAAGAT CATTTGTCCT ATTTCACTTT CATTGGAAGA
    GTTGCTGGGC TGGCAGTATT TCATGGGAAA CTTCTAGATG GTTTCTTCAT TCGACCGTTT
    TATAAAATGA TGCTGGGAAA GCAGATAACT CTGAATGACA TGGAATCTGT GGATAGCGAA
    TATTACAACT CTTTGAAATG GATCTTAGAA AATGACCCAA CTGAACTGGA CCTTATGTTC
    TGCATTGATG AAGAGAACTT TGGGCAGACA TATCAAGTGG ATCTGAAGCC CAACGGGTCA
    GAAATAATGG TAACGAACGA AAACAAGAGA GAGTACATCG ACCTCGTCAT CCAGTGGAGG
    TTCGTGAACA GGGTCCAGAA GCAGATGAAC GCCTTCCTGG AGGGATTCAC GGAACTGCTC
    CCTATTGATT TGATTAAAAT CTTTGATGAA AATGAACTGG AGTTGCTGAT GTGTGGCCTT
    GGCGACGTGG ATGTGAACGA CTGGCGACAA CATTCTATTT ACAAGAATGG CTACTGCCCG
    AACCACCCCG TGATCCAGTG GTTCTGGAAG GCTGTGCTGC TGATGGACGC CGAGAAGCGC
    ATCCGGTTGC TGCAGTTTGT CACAGGGACG TCCCGAGTAC CTATGAATGG ATTTGCCGAG
    CTTTATGGTT CCAATGGTCC TCAGCTGTTT ACAATAGAGC AATGGGGAAG TCCTGAAAAA
    CTGCCCAGAG CTCACACGTG CTTTAATCGC CTTGACTTAC CTCCGTATGA AACCTTCGAA
    GATTTACGAG AGAAGCTCCT CATGGCTGTG GAAAACGCCC AAGGCTTCGA GGGGGTGGAT
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa47561
    Clone ID Related Accession (Same CDS sequence) XM_005224351.3 , XM_010819012.2 , XM_015469185.1 , XM_010799401.2 , XM_024984479.1 , XM_015460157.1 , XM_024984477.1 , XM_024984478.1 , XM_015469184.1 , XM_015460156.1 , XM_015460155.1 , XM_015469186.1 , XM_015460156.2 , XM_024984476.1 , XM_010819012.3 , XM_005199752.3 , XM_005224351.4
    Accession Version XM_005224351.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2619bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104569.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005224351.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGCCCAGTGC CCCAGCTGGG
    AGAGCACGTT CATCAACTGT CACTGGTGGT GAGGCGCCAA CGCCATCAGT GGCCTATGTA
    CACACCACGC CGGGCCTGCC CTCGGGCTGG GAAGAGAGGA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCTATCAT GCAGCTCGCA
    GAAGACGGCG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CCGCCCCGCT GGAGGGCGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GATACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAGA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA CAACACAAAG
    ACCACGACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ACATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGCTGGG AAGAAAGGAT CCACTTGGAT
    GGCCGGACGT TTTACATTGA CCACAGCAGC CAGGTGTTAT GTGGCGAGGA CGATGCCAGA
    GACTCAGTGC CCTCGTACGA TAGCAAAATT ACTCAGTGGG AAGACCCAAG ACTGCAGAAC
    CCAGCTATTA CTGGTCCGGC TGTTCCTTAC TCCAGAGAAT TTAAGCAGAA ATATGACTAC
    TTTAGAAAGA AATTAAAGAA ACCTGCGGAT ATTCCAAATA GGTTTGAAAT GAAACTTCAC
    AGAAATAACA TATTTGAAGA GTCCTATCGG AGAATCATGT CTGTGAAAAG ACCAGATGTC
    CTGAAAGCTA GGCTGTGGAT TGAGTTTGAA TCGGAGAAAG GTCTGGACTA CGGGGGCGTG
    GCCAGAGAGT GGTTCTTCCT ACTGTCCAAG GAGATGTTCA ACCCTTACTA CGGCCTCTTC
    GAGTATTCGG CAACGGACAA CTACACACTT CAGATCAATC CCAATTCAGG CCTCTGTAAT
    GAAGATCATT TGTCCTATTT CACTTTCATT GGAAGAGTTG CTGGGCTGGC AGTATTTCAT
    GGGAAACTTC TAGATGGTTT CTTCATTCGA CCGTTTTATA AAATGATGCT GGGAAAGCAG
    ATAACTCTGA ATGACATGGA ATCTGTGGAT AGCGAATATT ACAACTCTTT GAAATGGATC
    TTAGAAAATG ACCCAACTGA ACTGGACCTT ATGTTCTGCA TTGATGAAGA GAACTTTGGG
    CAGACATATC AAGTGGATCT GAAGCCCAAC GGGTCAGAAA TAATGGTAAC GAACGAAAAC
    AAGAGAGAGT ACATCGACCT CGTCATCCAG TGGAGGTTCG TGAACAGGGT CCAGAAGCAG
    ATGAACGCCT TCCTGGAGGG ATTCACGGAA CTGCTCCCTA TTGATTTGAT TAAAATCTTT
    GATGAAAATG AACTGGAGTT GCTGATGTGT GGCCTTGGCG ACGTGGATGT GAACGACTGG
    CGACAACATT CTATTTACAA GAATGGCTAC TGCCCGAACC ACCCCGTGAT CCAGTGGTTC
    TGGAAGGCTG TGCTGCTGAT GGACGCCGAG AAGCGCATCC GGTTGCTGCA GTTTGTCACA
    GGGACGTCCC GAGTACCTAT GAATGGATTT GCCGAGCTTT ATGGTTCCAA TGGTCCTCAG
    CTGTTTACAA TAGAGCAATG GGGAAGTCCT GAAAAACTGC CCAGAGCTCA CACGTGCTTT
    AATCGCCTTG ACTTACCTCC GTATGAAACC TTCGAAGATT TACGAGAGAA GCTCCTCATG
    GCTGTGGAAA ACGCCCAAGG CTTCGAGGGG GTGGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005224408.3
    CDS559..3177
    Translation

    Target ORF information:

    RefSeq Version XM_005224351.3
    Organism Bos taurus(cattle)
    Definition Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005224351.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGCCCAGTGC CCCAGCTGGG
    AGAGCACGTT CATCAACTGT CACTGGTGGT GAGGCGCCAA CGCCATCAGT GGCCTATGTA
    CACACCACGC CGGGCCTGCC CTCGGGCTGG GAAGAGAGGA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCTATCAT GCAGCTCGCA
    GAAGACGGCG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CCGCCCCGCT GGAGGGCGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GATACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAGA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA CAACACAAAG
    ACCACGACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ACATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGCTGGG AAGAAAGGAT CCACTTGGAT
    GGCCGGACGT TTTACATTGA CCACAGCAGC CAGGTGTTAT GTGGCGAGGA CGATGCCAGA
    GACTCAGTGC CCTCGTACGA TAGCAAAATT ACTCAGTGGG AAGACCCAAG ACTGCAGAAC
    CCAGCTATTA CTGGTCCGGC TGTTCCTTAC TCCAGAGAAT TTAAGCAGAA ATATGACTAC
    TTTAGAAAGA AATTAAAGAA ACCTGCGGAT ATTCCAAATA GGTTTGAAAT GAAACTTCAC
    AGAAATAACA TATTTGAAGA GTCCTATCGG AGAATCATGT CTGTGAAAAG ACCAGATGTC
    CTGAAAGCTA GGCTGTGGAT TGAGTTTGAA TCGGAGAAAG GTCTGGACTA CGGGGGCGTG
    GCCAGAGAGT GGTTCTTCCT ACTGTCCAAG GAGATGTTCA ACCCTTACTA CGGCCTCTTC
    GAGTATTCGG CAACGGACAA CTACACACTT CAGATCAATC CCAATTCAGG CCTCTGTAAT
    GAAGATCATT TGTCCTATTT CACTTTCATT GGAAGAGTTG CTGGGCTGGC AGTATTTCAT
    GGGAAACTTC TAGATGGTTT CTTCATTCGA CCGTTTTATA AAATGATGCT GGGAAAGCAG
    ATAACTCTGA ATGACATGGA ATCTGTGGAT AGCGAATATT ACAACTCTTT GAAATGGATC
    TTAGAAAATG ACCCAACTGA ACTGGACCTT ATGTTCTGCA TTGATGAAGA GAACTTTGGG
    CAGACATATC AAGTGGATCT GAAGCCCAAC GGGTCAGAAA TAATGGTAAC GAACGAAAAC
    AAGAGAGAGT ACATCGACCT CGTCATCCAG TGGAGGTTCG TGAACAGGGT CCAGAAGCAG
    ATGAACGCCT TCCTGGAGGG ATTCACGGAA CTGCTCCCTA TTGATTTGAT TAAAATCTTT
    GATGAAAATG AACTGGAGTT GCTGATGTGT GGCCTTGGCG ACGTGGATGT GAACGACTGG
    CGACAACATT CTATTTACAA GAATGGCTAC TGCCCGAACC ACCCCGTGAT CCAGTGGTTC
    TGGAAGGCTG TGCTGCTGAT GGACGCCGAG AAGCGCATCC GGTTGCTGCA GTTTGTCACA
    GGGACGTCCC GAGTACCTAT GAATGGATTT GCCGAGCTTT ATGGTTCCAA TGGTCCTCAG
    CTGTTTACAA TAGAGCAATG GGGAAGTCCT GAAAAACTGC CCAGAGCTCA CACGTGCTTT
    AATCGCCTTG ACTTACCTCC GTATGAAACC TTCGAAGATT TACGAGAGAA GCTCCTCATG
    GCTGTGGAAA ACGCCCAAGG CTTCGAGGGG GTGGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa47561
    Clone ID Related Accession (Same CDS sequence) XM_005224351.3 , XM_010819012.2 , XM_015469185.1 , XM_010799401.2 , XM_024984479.1 , XM_015460157.1 , XM_024984477.1 , XM_024984478.1 , XM_015469184.1 , XM_015460156.1 , XM_015460155.1 , XM_015469186.1 , XM_015460156.2 , XM_024984476.1 , XM_010819012.3 , XM_005199752.3 , XM_005224351.4
    Accession Version XM_010819012.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2619bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104569.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010819012.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGCCCAGTGC CCCAGCTGGG
    AGAGCACGTT CATCAACTGT CACTGGTGGT GAGGCGCCAA CGCCATCAGT GGCCTATGTA
    CACACCACGC CGGGCCTGCC CTCGGGCTGG GAAGAGAGGA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCTATCAT GCAGCTCGCA
    GAAGACGGCG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CCGCCCCGCT GGAGGGCGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GATACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAGA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA CAACACAAAG
    ACCACGACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ACATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGCTGGG AAGAAAGGAT CCACTTGGAT
    GGCCGGACGT TTTACATTGA CCACAGCAGC CAGGTGTTAT GTGGCGAGGA CGATGCCAGA
    GACTCAGTGC CCTCGTACGA TAGCAAAATT ACTCAGTGGG AAGACCCAAG ACTGCAGAAC
    CCAGCTATTA CTGGTCCGGC TGTTCCTTAC TCCAGAGAAT TTAAGCAGAA ATATGACTAC
    TTTAGAAAGA AATTAAAGAA ACCTGCGGAT ATTCCAAATA GGTTTGAAAT GAAACTTCAC
    AGAAATAACA TATTTGAAGA GTCCTATCGG AGAATCATGT CTGTGAAAAG ACCAGATGTC
    CTGAAAGCTA GGCTGTGGAT TGAGTTTGAA TCGGAGAAAG GTCTGGACTA CGGGGGCGTG
    GCCAGAGAGT GGTTCTTCCT ACTGTCCAAG GAGATGTTCA ACCCTTACTA CGGCCTCTTC
    GAGTATTCGG CAACGGACAA CTACACACTT CAGATCAATC CCAATTCAGG CCTCTGTAAT
    GAAGATCATT TGTCCTATTT CACTTTCATT GGAAGAGTTG CTGGGCTGGC AGTATTTCAT
    GGGAAACTTC TAGATGGTTT CTTCATTCGA CCGTTTTATA AAATGATGCT GGGAAAGCAG
    ATAACTCTGA ATGACATGGA ATCTGTGGAT AGCGAATATT ACAACTCTTT GAAATGGATC
    TTAGAAAATG ACCCAACTGA ACTGGACCTT ATGTTCTGCA TTGATGAAGA GAACTTTGGG
    CAGACATATC AAGTGGATCT GAAGCCCAAC GGGTCAGAAA TAATGGTAAC GAACGAAAAC
    AAGAGAGAGT ACATCGACCT CGTCATCCAG TGGAGGTTCG TGAACAGGGT CCAGAAGCAG
    ATGAACGCCT TCCTGGAGGG ATTCACGGAA CTGCTCCCTA TTGATTTGAT TAAAATCTTT
    GATGAAAATG AACTGGAGTT GCTGATGTGT GGCCTTGGCG ACGTGGATGT GAACGACTGG
    CGACAACATT CTATTTACAA GAATGGCTAC TGCCCGAACC ACCCCGTGAT CCAGTGGTTC
    TGGAAGGCTG TGCTGCTGAT GGACGCCGAG AAGCGCATCC GGTTGCTGCA GTTTGTCACA
    GGGACGTCCC GAGTACCTAT GAATGGATTT GCCGAGCTTT ATGGTTCCAA TGGTCCTCAG
    CTGTTTACAA TAGAGCAATG GGGAAGTCCT GAAAAACTGC CCAGAGCTCA CACGTGCTTT
    AATCGCCTTG ACTTACCTCC GTATGAAACC TTCGAAGATT TACGAGAGAA GCTCCTCATG
    GCTGTGGAAA ACGCCCAAGG CTTCGAGGGG GTGGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010817314.1
    CDS302..2920
    Translation

    Target ORF information:

    RefSeq Version XM_010819012.2
    Organism Bos taurus(cattle)
    Definition Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010819012.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGCCCAGTGC CCCAGCTGGG
    AGAGCACGTT CATCAACTGT CACTGGTGGT GAGGCGCCAA CGCCATCAGT GGCCTATGTA
    CACACCACGC CGGGCCTGCC CTCGGGCTGG GAAGAGAGGA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCTATCAT GCAGCTCGCA
    GAAGACGGCG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CCGCCCCGCT GGAGGGCGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GATACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAGA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA CAACACAAAG
    ACCACGACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ACATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGCTGGG AAGAAAGGAT CCACTTGGAT
    GGCCGGACGT TTTACATTGA CCACAGCAGC CAGGTGTTAT GTGGCGAGGA CGATGCCAGA
    GACTCAGTGC CCTCGTACGA TAGCAAAATT ACTCAGTGGG AAGACCCAAG ACTGCAGAAC
    CCAGCTATTA CTGGTCCGGC TGTTCCTTAC TCCAGAGAAT TTAAGCAGAA ATATGACTAC
    TTTAGAAAGA AATTAAAGAA ACCTGCGGAT ATTCCAAATA GGTTTGAAAT GAAACTTCAC
    AGAAATAACA TATTTGAAGA GTCCTATCGG AGAATCATGT CTGTGAAAAG ACCAGATGTC
    CTGAAAGCTA GGCTGTGGAT TGAGTTTGAA TCGGAGAAAG GTCTGGACTA CGGGGGCGTG
    GCCAGAGAGT GGTTCTTCCT ACTGTCCAAG GAGATGTTCA ACCCTTACTA CGGCCTCTTC
    GAGTATTCGG CAACGGACAA CTACACACTT CAGATCAATC CCAATTCAGG CCTCTGTAAT
    GAAGATCATT TGTCCTATTT CACTTTCATT GGAAGAGTTG CTGGGCTGGC AGTATTTCAT
    GGGAAACTTC TAGATGGTTT CTTCATTCGA CCGTTTTATA AAATGATGCT GGGAAAGCAG
    ATAACTCTGA ATGACATGGA ATCTGTGGAT AGCGAATATT ACAACTCTTT GAAATGGATC
    TTAGAAAATG ACCCAACTGA ACTGGACCTT ATGTTCTGCA TTGATGAAGA GAACTTTGGG
    CAGACATATC AAGTGGATCT GAAGCCCAAC GGGTCAGAAA TAATGGTAAC GAACGAAAAC
    AAGAGAGAGT ACATCGACCT CGTCATCCAG TGGAGGTTCG TGAACAGGGT CCAGAAGCAG
    ATGAACGCCT TCCTGGAGGG ATTCACGGAA CTGCTCCCTA TTGATTTGAT TAAAATCTTT
    GATGAAAATG AACTGGAGTT GCTGATGTGT GGCCTTGGCG ACGTGGATGT GAACGACTGG
    CGACAACATT CTATTTACAA GAATGGCTAC TGCCCGAACC ACCCCGTGAT CCAGTGGTTC
    TGGAAGGCTG TGCTGCTGAT GGACGCCGAG AAGCGCATCC GGTTGCTGCA GTTTGTCACA
    GGGACGTCCC GAGTACCTAT GAATGGATTT GCCGAGCTTT ATGGTTCCAA TGGTCCTCAG
    CTGTTTACAA TAGAGCAATG GGGAAGTCCT GAAAAACTGC CCAGAGCTCA CACGTGCTTT
    AATCGCCTTG ACTTACCTCC GTATGAAACC TTCGAAGATT TACGAGAGAA GCTCCTCATG
    GCTGTGGAAA ACGCCCAAGG CTTCGAGGGG GTGGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa47561
    Clone ID Related Accession (Same CDS sequence) XM_005224351.3 , XM_010819012.2 , XM_015469185.1 , XM_010799401.2 , XM_024984479.1 , XM_015460157.1 , XM_024984477.1 , XM_024984478.1 , XM_015469184.1 , XM_015460156.1 , XM_015460155.1 , XM_015469186.1 , XM_015460156.2 , XM_024984476.1 , XM_010819012.3 , XM_005199752.3 , XM_005224351.4
    Accession Version XM_015469185.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2619bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646636.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGCCCAGTGC CCCAGCTGGG
    AGAGCACGTT CATCAACTGT CACTGGTGGT GAGGCGCCAA CGCCATCAGT GGCCTATGTA
    CACACCACGC CGGGCCTGCC CTCGGGCTGG GAAGAGAGGA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCTATCAT GCAGCTCGCA
    GAAGACGGCG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CCGCCCCGCT GGAGGGCGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GATACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAGA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA CAACACAAAG
    ACCACGACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ACATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGCTGGG AAGAAAGGAT CCACTTGGAT
    GGCCGGACGT TTTACATTGA CCACAGCAGC CAGGTGTTAT GTGGCGAGGA CGATGCCAGA
    GACTCAGTGC CCTCGTACGA TAGCAAAATT ACTCAGTGGG AAGACCCAAG ACTGCAGAAC
    CCAGCTATTA CTGGTCCGGC TGTTCCTTAC TCCAGAGAAT TTAAGCAGAA ATATGACTAC
    TTTAGAAAGA AATTAAAGAA ACCTGCGGAT ATTCCAAATA GGTTTGAAAT GAAACTTCAC
    AGAAATAACA TATTTGAAGA GTCCTATCGG AGAATCATGT CTGTGAAAAG ACCAGATGTC
    CTGAAAGCTA GGCTGTGGAT TGAGTTTGAA TCGGAGAAAG GTCTGGACTA CGGGGGCGTG
    GCCAGAGAGT GGTTCTTCCT ACTGTCCAAG GAGATGTTCA ACCCTTACTA CGGCCTCTTC
    GAGTATTCGG CAACGGACAA CTACACACTT CAGATCAATC CCAATTCAGG CCTCTGTAAT
    GAAGATCATT TGTCCTATTT CACTTTCATT GGAAGAGTTG CTGGGCTGGC AGTATTTCAT
    GGGAAACTTC TAGATGGTTT CTTCATTCGA CCGTTTTATA AAATGATGCT GGGAAAGCAG
    ATAACTCTGA ATGACATGGA ATCTGTGGAT AGCGAATATT ACAACTCTTT GAAATGGATC
    TTAGAAAATG ACCCAACTGA ACTGGACCTT ATGTTCTGCA TTGATGAAGA GAACTTTGGG
    CAGACATATC AAGTGGATCT GAAGCCCAAC GGGTCAGAAA TAATGGTAAC GAACGAAAAC
    AAGAGAGAGT ACATCGACCT CGTCATCCAG TGGAGGTTCG TGAACAGGGT CCAGAAGCAG
    ATGAACGCCT TCCTGGAGGG ATTCACGGAA CTGCTCCCTA TTGATTTGAT TAAAATCTTT
    GATGAAAATG AACTGGAGTT GCTGATGTGT GGCCTTGGCG ACGTGGATGT GAACGACTGG
    CGACAACATT CTATTTACAA GAATGGCTAC TGCCCGAACC ACCCCGTGAT CCAGTGGTTC
    TGGAAGGCTG TGCTGCTGAT GGACGCCGAG AAGCGCATCC GGTTGCTGCA GTTTGTCACA
    GGGACGTCCC GAGTACCTAT GAATGGATTT GCCGAGCTTT ATGGTTCCAA TGGTCCTCAG
    CTGTTTACAA TAGAGCAATG GGGAAGTCCT GAAAAACTGC CCAGAGCTCA CACGTGCTTT
    AATCGCCTTG ACTTACCTCC GTATGAAACC TTCGAAGATT TACGAGAGAA GCTCCTCATG
    GCTGTGGAAA ACGCCCAAGG CTTCGAGGGG GTGGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015324671.1
    CDS1086..3704
    Translation

    Target ORF information:

    RefSeq Version XM_015469185.1
    Organism Bos taurus(cattle)
    Definition Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015469185.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGCCCAGTGC CCCAGCTGGG
    AGAGCACGTT CATCAACTGT CACTGGTGGT GAGGCGCCAA CGCCATCAGT GGCCTATGTA
    CACACCACGC CGGGCCTGCC CTCGGGCTGG GAAGAGAGGA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCTATCAT GCAGCTCGCA
    GAAGACGGCG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CCGCCCCGCT GGAGGGCGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GATACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAGA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA CAACACAAAG
    ACCACGACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ACATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGCTGGG AAGAAAGGAT CCACTTGGAT
    GGCCGGACGT TTTACATTGA CCACAGCAGC CAGGTGTTAT GTGGCGAGGA CGATGCCAGA
    GACTCAGTGC CCTCGTACGA TAGCAAAATT ACTCAGTGGG AAGACCCAAG ACTGCAGAAC
    CCAGCTATTA CTGGTCCGGC TGTTCCTTAC TCCAGAGAAT TTAAGCAGAA ATATGACTAC
    TTTAGAAAGA AATTAAAGAA ACCTGCGGAT ATTCCAAATA GGTTTGAAAT GAAACTTCAC
    AGAAATAACA TATTTGAAGA GTCCTATCGG AGAATCATGT CTGTGAAAAG ACCAGATGTC
    CTGAAAGCTA GGCTGTGGAT TGAGTTTGAA TCGGAGAAAG GTCTGGACTA CGGGGGCGTG
    GCCAGAGAGT GGTTCTTCCT ACTGTCCAAG GAGATGTTCA ACCCTTACTA CGGCCTCTTC
    GAGTATTCGG CAACGGACAA CTACACACTT CAGATCAATC CCAATTCAGG CCTCTGTAAT
    GAAGATCATT TGTCCTATTT CACTTTCATT GGAAGAGTTG CTGGGCTGGC AGTATTTCAT
    GGGAAACTTC TAGATGGTTT CTTCATTCGA CCGTTTTATA AAATGATGCT GGGAAAGCAG
    ATAACTCTGA ATGACATGGA ATCTGTGGAT AGCGAATATT ACAACTCTTT GAAATGGATC
    TTAGAAAATG ACCCAACTGA ACTGGACCTT ATGTTCTGCA TTGATGAAGA GAACTTTGGG
    CAGACATATC AAGTGGATCT GAAGCCCAAC GGGTCAGAAA TAATGGTAAC GAACGAAAAC
    AAGAGAGAGT ACATCGACCT CGTCATCCAG TGGAGGTTCG TGAACAGGGT CCAGAAGCAG
    ATGAACGCCT TCCTGGAGGG ATTCACGGAA CTGCTCCCTA TTGATTTGAT TAAAATCTTT
    GATGAAAATG AACTGGAGTT GCTGATGTGT GGCCTTGGCG ACGTGGATGT GAACGACTGG
    CGACAACATT CTATTTACAA GAATGGCTAC TGCCCGAACC ACCCCGTGAT CCAGTGGTTC
    TGGAAGGCTG TGCTGCTGAT GGACGCCGAG AAGCGCATCC GGTTGCTGCA GTTTGTCACA
    GGGACGTCCC GAGTACCTAT GAATGGATTT GCCGAGCTTT ATGGTTCCAA TGGTCCTCAG
    CTGTTTACAA TAGAGCAATG GGGAAGTCCT GAAAAACTGC CCAGAGCTCA CACGTGCTTT
    AATCGCCTTG ACTTACCTCC GTATGAAACC TTCGAAGATT TACGAGAGAA GCTCCTCATG
    GCTGTGGAAA ACGCCCAAGG CTTCGAGGGG GTGGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa47561
    Clone ID Related Accession (Same CDS sequence) XM_005224351.3 , XM_010819012.2 , XM_015469185.1 , XM_010799401.2 , XM_024984479.1 , XM_015460157.1 , XM_024984477.1 , XM_024984478.1 , XM_015469184.1 , XM_015460156.1 , XM_015460155.1 , XM_015469186.1 , XM_015460156.2 , XM_024984476.1 , XM_010819012.3 , XM_005199752.3 , XM_005224351.4
    Accession Version XM_010799401.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2619bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646636.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010799401.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGCCCAGTGC CCCAGCTGGG
    AGAGCACGTT CATCAACTGT CACTGGTGGT GAGGCGCCAA CGCCATCAGT GGCCTATGTA
    CACACCACGC CGGGCCTGCC CTCGGGCTGG GAAGAGAGGA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCTATCAT GCAGCTCGCA
    GAAGACGGCG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CCGCCCCGCT GGAGGGCGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GATACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAGA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA CAACACAAAG
    ACCACGACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ACATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGCTGGG AAGAAAGGAT CCACTTGGAT
    GGCCGGACGT TTTACATTGA CCACAGCAGC CAGGTGTTAT GTGGCGAGGA CGATGCCAGA
    GACTCAGTGC CCTCGTACGA TAGCAAAATT ACTCAGTGGG AAGACCCAAG ACTGCAGAAC
    CCAGCTATTA CTGGTCCGGC TGTTCCTTAC TCCAGAGAAT TTAAGCAGAA ATATGACTAC
    TTTAGAAAGA AATTAAAGAA ACCTGCGGAT ATTCCAAATA GGTTTGAAAT GAAACTTCAC
    AGAAATAACA TATTTGAAGA GTCCTATCGG AGAATCATGT CTGTGAAAAG ACCAGATGTC
    CTGAAAGCTA GGCTGTGGAT TGAGTTTGAA TCGGAGAAAG GTCTGGACTA CGGGGGCGTG
    GCCAGAGAGT GGTTCTTCCT ACTGTCCAAG GAGATGTTCA ACCCTTACTA CGGCCTCTTC
    GAGTATTCGG CAACGGACAA CTACACACTT CAGATCAATC CCAATTCAGG CCTCTGTAAT
    GAAGATCATT TGTCCTATTT CACTTTCATT GGAAGAGTTG CTGGGCTGGC AGTATTTCAT
    GGGAAACTTC TAGATGGTTT CTTCATTCGA CCGTTTTATA AAATGATGCT GGGAAAGCAG
    ATAACTCTGA ATGACATGGA ATCTGTGGAT AGCGAATATT ACAACTCTTT GAAATGGATC
    TTAGAAAATG ACCCAACTGA ACTGGACCTT ATGTTCTGCA TTGATGAAGA GAACTTTGGG
    CAGACATATC AAGTGGATCT GAAGCCCAAC GGGTCAGAAA TAATGGTAAC GAACGAAAAC
    AAGAGAGAGT ACATCGACCT CGTCATCCAG TGGAGGTTCG TGAACAGGGT CCAGAAGCAG
    ATGAACGCCT TCCTGGAGGG ATTCACGGAA CTGCTCCCTA TTGATTTGAT TAAAATCTTT
    GATGAAAATG AACTGGAGTT GCTGATGTGT GGCCTTGGCG ACGTGGATGT GAACGACTGG
    CGACAACATT CTATTTACAA GAATGGCTAC TGCCCGAACC ACCCCGTGAT CCAGTGGTTC
    TGGAAGGCTG TGCTGCTGAT GGACGCCGAG AAGCGCATCC GGTTGCTGCA GTTTGTCACA
    GGGACGTCCC GAGTACCTAT GAATGGATTT GCCGAGCTTT ATGGTTCCAA TGGTCCTCAG
    CTGTTTACAA TAGAGCAATG GGGAAGTCCT GAAAAACTGC CCAGAGCTCA CACGTGCTTT
    AATCGCCTTG ACTTACCTCC GTATGAAACC TTCGAAGATT TACGAGAGAA GCTCCTCATG
    GCTGTGGAAA ACGCCCAAGG CTTCGAGGGG GTGGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010797703.1
    CDS302..2920
    Translation

    Target ORF information:

    RefSeq Version XM_010799401.2
    Organism Bos taurus(cattle)
    Definition Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010799401.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGCCCAGTGC CCCAGCTGGG
    AGAGCACGTT CATCAACTGT CACTGGTGGT GAGGCGCCAA CGCCATCAGT GGCCTATGTA
    CACACCACGC CGGGCCTGCC CTCGGGCTGG GAAGAGAGGA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCTATCAT GCAGCTCGCA
    GAAGACGGCG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CCGCCCCGCT GGAGGGCGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GATACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAGA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA CAACACAAAG
    ACCACGACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ACATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGCTGGG AAGAAAGGAT CCACTTGGAT
    GGCCGGACGT TTTACATTGA CCACAGCAGC CAGGTGTTAT GTGGCGAGGA CGATGCCAGA
    GACTCAGTGC CCTCGTACGA TAGCAAAATT ACTCAGTGGG AAGACCCAAG ACTGCAGAAC
    CCAGCTATTA CTGGTCCGGC TGTTCCTTAC TCCAGAGAAT TTAAGCAGAA ATATGACTAC
    TTTAGAAAGA AATTAAAGAA ACCTGCGGAT ATTCCAAATA GGTTTGAAAT GAAACTTCAC
    AGAAATAACA TATTTGAAGA GTCCTATCGG AGAATCATGT CTGTGAAAAG ACCAGATGTC
    CTGAAAGCTA GGCTGTGGAT TGAGTTTGAA TCGGAGAAAG GTCTGGACTA CGGGGGCGTG
    GCCAGAGAGT GGTTCTTCCT ACTGTCCAAG GAGATGTTCA ACCCTTACTA CGGCCTCTTC
    GAGTATTCGG CAACGGACAA CTACACACTT CAGATCAATC CCAATTCAGG CCTCTGTAAT
    GAAGATCATT TGTCCTATTT CACTTTCATT GGAAGAGTTG CTGGGCTGGC AGTATTTCAT
    GGGAAACTTC TAGATGGTTT CTTCATTCGA CCGTTTTATA AAATGATGCT GGGAAAGCAG
    ATAACTCTGA ATGACATGGA ATCTGTGGAT AGCGAATATT ACAACTCTTT GAAATGGATC
    TTAGAAAATG ACCCAACTGA ACTGGACCTT ATGTTCTGCA TTGATGAAGA GAACTTTGGG
    CAGACATATC AAGTGGATCT GAAGCCCAAC GGGTCAGAAA TAATGGTAAC GAACGAAAAC
    AAGAGAGAGT ACATCGACCT CGTCATCCAG TGGAGGTTCG TGAACAGGGT CCAGAAGCAG
    ATGAACGCCT TCCTGGAGGG ATTCACGGAA CTGCTCCCTA TTGATTTGAT TAAAATCTTT
    GATGAAAATG AACTGGAGTT GCTGATGTGT GGCCTTGGCG ACGTGGATGT GAACGACTGG
    CGACAACATT CTATTTACAA GAATGGCTAC TGCCCGAACC ACCCCGTGAT CCAGTGGTTC
    TGGAAGGCTG TGCTGCTGAT GGACGCCGAG AAGCGCATCC GGTTGCTGCA GTTTGTCACA
    GGGACGTCCC GAGTACCTAT GAATGGATTT GCCGAGCTTT ATGGTTCCAA TGGTCCTCAG
    CTGTTTACAA TAGAGCAATG GGGAAGTCCT GAAAAACTGC CCAGAGCTCA CACGTGCTTT
    AATCGCCTTG ACTTACCTCC GTATGAAACC TTCGAAGATT TACGAGAGAA GCTCCTCATG
    GCTGTGGAAA ACGCCCAAGG CTTCGAGGGG GTGGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa47561
    Clone ID Related Accession (Same CDS sequence) XM_005224351.3 , XM_010819012.2 , XM_015469185.1 , XM_010799401.2 , XM_024984479.1 , XM_015460157.1 , XM_024984477.1 , XM_024984478.1 , XM_015469184.1 , XM_015460156.1 , XM_015460155.1 , XM_015469186.1 , XM_015460156.2 , XM_024984476.1 , XM_010819012.3 , XM_005199752.3 , XM_005224351.4
    Accession Version XM_024984479.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2619bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGCCCAGTGC CCCAGCTGGG
    AGAGCACGTT CATCAACTGT CACTGGTGGT GAGGCGCCAA CGCCATCAGT GGCCTATGTA
    CACACCACGC CGGGCCTGCC CTCGGGCTGG GAAGAGAGGA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCTATCAT GCAGCTCGCA
    GAAGACGGCG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CCGCCCCGCT GGAGGGCGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GATACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAGA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA CAACACAAAG
    ACCACGACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ACATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGCTGGG AAGAAAGGAT CCACTTGGAT
    GGCCGGACGT TTTACATTGA CCACAGCAGC CAGGTGTTAT GTGGCGAGGA CGATGCCAGA
    GACTCAGTGC CCTCGTACGA TAGCAAAATT ACTCAGTGGG AAGACCCAAG ACTGCAGAAC
    CCAGCTATTA CTGGTCCGGC TGTTCCTTAC TCCAGAGAAT TTAAGCAGAA ATATGACTAC
    TTTAGAAAGA AATTAAAGAA ACCTGCGGAT ATTCCAAATA GGTTTGAAAT GAAACTTCAC
    AGAAATAACA TATTTGAAGA GTCCTATCGG AGAATCATGT CTGTGAAAAG ACCAGATGTC
    CTGAAAGCTA GGCTGTGGAT TGAGTTTGAA TCGGAGAAAG GTCTGGACTA CGGGGGCGTG
    GCCAGAGAGT GGTTCTTCCT ACTGTCCAAG GAGATGTTCA ACCCTTACTA CGGCCTCTTC
    GAGTATTCGG CAACGGACAA CTACACACTT CAGATCAATC CCAATTCAGG CCTCTGTAAT
    GAAGATCATT TGTCCTATTT CACTTTCATT GGAAGAGTTG CTGGGCTGGC AGTATTTCAT
    GGGAAACTTC TAGATGGTTT CTTCATTCGA CCGTTTTATA AAATGATGCT GGGAAAGCAG
    ATAACTCTGA ATGACATGGA ATCTGTGGAT AGCGAATATT ACAACTCTTT GAAATGGATC
    TTAGAAAATG ACCCAACTGA ACTGGACCTT ATGTTCTGCA TTGATGAAGA GAACTTTGGG
    CAGACATATC AAGTGGATCT GAAGCCCAAC GGGTCAGAAA TAATGGTAAC GAACGAAAAC
    AAGAGAGAGT ACATCGACCT CGTCATCCAG TGGAGGTTCG TGAACAGGGT CCAGAAGCAG
    ATGAACGCCT TCCTGGAGGG ATTCACGGAA CTGCTCCCTA TTGATTTGAT TAAAATCTTT
    GATGAAAATG AACTGGAGTT GCTGATGTGT GGCCTTGGCG ACGTGGATGT GAACGACTGG
    CGACAACATT CTATTTACAA GAATGGCTAC TGCCCGAACC ACCCCGTGAT CCAGTGGTTC
    TGGAAGGCTG TGCTGCTGAT GGACGCCGAG AAGCGCATCC GGTTGCTGCA GTTTGTCACA
    GGGACGTCCC GAGTACCTAT GAATGGATTT GCCGAGCTTT ATGGTTCCAA TGGTCCTCAG
    CTGTTTACAA TAGAGCAATG GGGAAGTCCT GAAAAACTGC CCAGAGCTCA CACGTGCTTT
    AATCGCCTTG ACTTACCTCC GTATGAAACC TTCGAAGATT TACGAGAGAA GCTCCTCATG
    GCTGTGGAAA ACGCCCAAGG CTTCGAGGGG GTGGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024840247.1
    CDS239..2857
    Translation

    Target ORF information:

    RefSeq Version XM_024984479.1
    Organism Bos taurus(cattle)
    Definition Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024984479.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGCCCAGTGC CCCAGCTGGG
    AGAGCACGTT CATCAACTGT CACTGGTGGT GAGGCGCCAA CGCCATCAGT GGCCTATGTA
    CACACCACGC CGGGCCTGCC CTCGGGCTGG GAAGAGAGGA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCTATCAT GCAGCTCGCA
    GAAGACGGCG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CCGCCCCGCT GGAGGGCGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GATACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAGA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA CAACACAAAG
    ACCACGACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ACATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGCTGGG AAGAAAGGAT CCACTTGGAT
    GGCCGGACGT TTTACATTGA CCACAGCAGC CAGGTGTTAT GTGGCGAGGA CGATGCCAGA
    GACTCAGTGC CCTCGTACGA TAGCAAAATT ACTCAGTGGG AAGACCCAAG ACTGCAGAAC
    CCAGCTATTA CTGGTCCGGC TGTTCCTTAC TCCAGAGAAT TTAAGCAGAA ATATGACTAC
    TTTAGAAAGA AATTAAAGAA ACCTGCGGAT ATTCCAAATA GGTTTGAAAT GAAACTTCAC
    AGAAATAACA TATTTGAAGA GTCCTATCGG AGAATCATGT CTGTGAAAAG ACCAGATGTC
    CTGAAAGCTA GGCTGTGGAT TGAGTTTGAA TCGGAGAAAG GTCTGGACTA CGGGGGCGTG
    GCCAGAGAGT GGTTCTTCCT ACTGTCCAAG GAGATGTTCA ACCCTTACTA CGGCCTCTTC
    GAGTATTCGG CAACGGACAA CTACACACTT CAGATCAATC CCAATTCAGG CCTCTGTAAT
    GAAGATCATT TGTCCTATTT CACTTTCATT GGAAGAGTTG CTGGGCTGGC AGTATTTCAT
    GGGAAACTTC TAGATGGTTT CTTCATTCGA CCGTTTTATA AAATGATGCT GGGAAAGCAG
    ATAACTCTGA ATGACATGGA ATCTGTGGAT AGCGAATATT ACAACTCTTT GAAATGGATC
    TTAGAAAATG ACCCAACTGA ACTGGACCTT ATGTTCTGCA TTGATGAAGA GAACTTTGGG
    CAGACATATC AAGTGGATCT GAAGCCCAAC GGGTCAGAAA TAATGGTAAC GAACGAAAAC
    AAGAGAGAGT ACATCGACCT CGTCATCCAG TGGAGGTTCG TGAACAGGGT CCAGAAGCAG
    ATGAACGCCT TCCTGGAGGG ATTCACGGAA CTGCTCCCTA TTGATTTGAT TAAAATCTTT
    GATGAAAATG AACTGGAGTT GCTGATGTGT GGCCTTGGCG ACGTGGATGT GAACGACTGG
    CGACAACATT CTATTTACAA GAATGGCTAC TGCCCGAACC ACCCCGTGAT CCAGTGGTTC
    TGGAAGGCTG TGCTGCTGAT GGACGCCGAG AAGCGCATCC GGTTGCTGCA GTTTGTCACA
    GGGACGTCCC GAGTACCTAT GAATGGATTT GCCGAGCTTT ATGGTTCCAA TGGTCCTCAG
    CTGTTTACAA TAGAGCAATG GGGAAGTCCT GAAAAACTGC CCAGAGCTCA CACGTGCTTT
    AATCGCCTTG ACTTACCTCC GTATGAAACC TTCGAAGATT TACGAGAGAA GCTCCTCATG
    GCTGTGGAAA ACGCCCAAGG CTTCGAGGGG GTGGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa47561
    Clone ID Related Accession (Same CDS sequence) XM_005224351.3 , XM_010819012.2 , XM_015469185.1 , XM_010799401.2 , XM_024984479.1 , XM_015460157.1 , XM_024984477.1 , XM_024984478.1 , XM_015469184.1 , XM_015460156.1 , XM_015460155.1 , XM_015469186.1 , XM_015460156.2 , XM_024984476.1 , XM_010819012.3 , XM_005199752.3 , XM_005224351.4
    Accession Version XM_015460157.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2619bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104569.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGCCCAGTGC CCCAGCTGGG
    AGAGCACGTT CATCAACTGT CACTGGTGGT GAGGCGCCAA CGCCATCAGT GGCCTATGTA
    CACACCACGC CGGGCCTGCC CTCGGGCTGG GAAGAGAGGA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCTATCAT GCAGCTCGCA
    GAAGACGGCG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CCGCCCCGCT GGAGGGCGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GATACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAGA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA CAACACAAAG
    ACCACGACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ACATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGCTGGG AAGAAAGGAT CCACTTGGAT
    GGCCGGACGT TTTACATTGA CCACAGCAGC CAGGTGTTAT GTGGCGAGGA CGATGCCAGA
    GACTCAGTGC CCTCGTACGA TAGCAAAATT ACTCAGTGGG AAGACCCAAG ACTGCAGAAC
    CCAGCTATTA CTGGTCCGGC TGTTCCTTAC TCCAGAGAAT TTAAGCAGAA ATATGACTAC
    TTTAGAAAGA AATTAAAGAA ACCTGCGGAT ATTCCAAATA GGTTTGAAAT GAAACTTCAC
    AGAAATAACA TATTTGAAGA GTCCTATCGG AGAATCATGT CTGTGAAAAG ACCAGATGTC
    CTGAAAGCTA GGCTGTGGAT TGAGTTTGAA TCGGAGAAAG GTCTGGACTA CGGGGGCGTG
    GCCAGAGAGT GGTTCTTCCT ACTGTCCAAG GAGATGTTCA ACCCTTACTA CGGCCTCTTC
    GAGTATTCGG CAACGGACAA CTACACACTT CAGATCAATC CCAATTCAGG CCTCTGTAAT
    GAAGATCATT TGTCCTATTT CACTTTCATT GGAAGAGTTG CTGGGCTGGC AGTATTTCAT
    GGGAAACTTC TAGATGGTTT CTTCATTCGA CCGTTTTATA AAATGATGCT GGGAAAGCAG
    ATAACTCTGA ATGACATGGA ATCTGTGGAT AGCGAATATT ACAACTCTTT GAAATGGATC
    TTAGAAAATG ACCCAACTGA ACTGGACCTT ATGTTCTGCA TTGATGAAGA GAACTTTGGG
    CAGACATATC AAGTGGATCT GAAGCCCAAC GGGTCAGAAA TAATGGTAAC GAACGAAAAC
    AAGAGAGAGT ACATCGACCT CGTCATCCAG TGGAGGTTCG TGAACAGGGT CCAGAAGCAG
    ATGAACGCCT TCCTGGAGGG ATTCACGGAA CTGCTCCCTA TTGATTTGAT TAAAATCTTT
    GATGAAAATG AACTGGAGTT GCTGATGTGT GGCCTTGGCG ACGTGGATGT GAACGACTGG
    CGACAACATT CTATTTACAA GAATGGCTAC TGCCCGAACC ACCCCGTGAT CCAGTGGTTC
    TGGAAGGCTG TGCTGCTGAT GGACGCCGAG AAGCGCATCC GGTTGCTGCA GTTTGTCACA
    GGGACGTCCC GAGTACCTAT GAATGGATTT GCCGAGCTTT ATGGTTCCAA TGGTCCTCAG
    CTGTTTACAA TAGAGCAATG GGGAAGTCCT GAAAAACTGC CCAGAGCTCA CACGTGCTTT
    AATCGCCTTG ACTTACCTCC GTATGAAACC TTCGAAGATT TACGAGAGAA GCTCCTCATG
    GCTGTGGAAA ACGCCCAAGG CTTCGAGGGG GTGGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015315643.1
    CDS392..3010
    Translation

    Target ORF information:

    RefSeq Version XM_015460157.1
    Organism Bos taurus(cattle)
    Definition Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015460157.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGCCCAGTGC CCCAGCTGGG
    AGAGCACGTT CATCAACTGT CACTGGTGGT GAGGCGCCAA CGCCATCAGT GGCCTATGTA
    CACACCACGC CGGGCCTGCC CTCGGGCTGG GAAGAGAGGA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCTATCAT GCAGCTCGCA
    GAAGACGGCG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CCGCCCCGCT GGAGGGCGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GATACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAGA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA CAACACAAAG
    ACCACGACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ACATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGCTGGG AAGAAAGGAT CCACTTGGAT
    GGCCGGACGT TTTACATTGA CCACAGCAGC CAGGTGTTAT GTGGCGAGGA CGATGCCAGA
    GACTCAGTGC CCTCGTACGA TAGCAAAATT ACTCAGTGGG AAGACCCAAG ACTGCAGAAC
    CCAGCTATTA CTGGTCCGGC TGTTCCTTAC TCCAGAGAAT TTAAGCAGAA ATATGACTAC
    TTTAGAAAGA AATTAAAGAA ACCTGCGGAT ATTCCAAATA GGTTTGAAAT GAAACTTCAC
    AGAAATAACA TATTTGAAGA GTCCTATCGG AGAATCATGT CTGTGAAAAG ACCAGATGTC
    CTGAAAGCTA GGCTGTGGAT TGAGTTTGAA TCGGAGAAAG GTCTGGACTA CGGGGGCGTG
    GCCAGAGAGT GGTTCTTCCT ACTGTCCAAG GAGATGTTCA ACCCTTACTA CGGCCTCTTC
    GAGTATTCGG CAACGGACAA CTACACACTT CAGATCAATC CCAATTCAGG CCTCTGTAAT
    GAAGATCATT TGTCCTATTT CACTTTCATT GGAAGAGTTG CTGGGCTGGC AGTATTTCAT
    GGGAAACTTC TAGATGGTTT CTTCATTCGA CCGTTTTATA AAATGATGCT GGGAAAGCAG
    ATAACTCTGA ATGACATGGA ATCTGTGGAT AGCGAATATT ACAACTCTTT GAAATGGATC
    TTAGAAAATG ACCCAACTGA ACTGGACCTT ATGTTCTGCA TTGATGAAGA GAACTTTGGG
    CAGACATATC AAGTGGATCT GAAGCCCAAC GGGTCAGAAA TAATGGTAAC GAACGAAAAC
    AAGAGAGAGT ACATCGACCT CGTCATCCAG TGGAGGTTCG TGAACAGGGT CCAGAAGCAG
    ATGAACGCCT TCCTGGAGGG ATTCACGGAA CTGCTCCCTA TTGATTTGAT TAAAATCTTT
    GATGAAAATG AACTGGAGTT GCTGATGTGT GGCCTTGGCG ACGTGGATGT GAACGACTGG
    CGACAACATT CTATTTACAA GAATGGCTAC TGCCCGAACC ACCCCGTGAT CCAGTGGTTC
    TGGAAGGCTG TGCTGCTGAT GGACGCCGAG AAGCGCATCC GGTTGCTGCA GTTTGTCACA
    GGGACGTCCC GAGTACCTAT GAATGGATTT GCCGAGCTTT ATGGTTCCAA TGGTCCTCAG
    CTGTTTACAA TAGAGCAATG GGGAAGTCCT GAAAAACTGC CCAGAGCTCA CACGTGCTTT
    AATCGCCTTG ACTTACCTCC GTATGAAACC TTCGAAGATT TACGAGAGAA GCTCCTCATG
    GCTGTGGAAA ACGCCCAAGG CTTCGAGGGG GTGGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa47561
    Clone ID Related Accession (Same CDS sequence) XM_005224351.3 , XM_010819012.2 , XM_015469185.1 , XM_010799401.2 , XM_024984479.1 , XM_015460157.1 , XM_024984477.1 , XM_024984478.1 , XM_015469184.1 , XM_015460156.1 , XM_015460155.1 , XM_015469186.1 , XM_015460156.2 , XM_024984476.1 , XM_010819012.3 , XM_005199752.3 , XM_005224351.4
    Accession Version XM_024984477.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2619bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGCCCAGTGC CCCAGCTGGG
    AGAGCACGTT CATCAACTGT CACTGGTGGT GAGGCGCCAA CGCCATCAGT GGCCTATGTA
    CACACCACGC CGGGCCTGCC CTCGGGCTGG GAAGAGAGGA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCTATCAT GCAGCTCGCA
    GAAGACGGCG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CCGCCCCGCT GGAGGGCGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GATACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAGA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA CAACACAAAG
    ACCACGACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ACATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGCTGGG AAGAAAGGAT CCACTTGGAT
    GGCCGGACGT TTTACATTGA CCACAGCAGC CAGGTGTTAT GTGGCGAGGA CGATGCCAGA
    GACTCAGTGC CCTCGTACGA TAGCAAAATT ACTCAGTGGG AAGACCCAAG ACTGCAGAAC
    CCAGCTATTA CTGGTCCGGC TGTTCCTTAC TCCAGAGAAT TTAAGCAGAA ATATGACTAC
    TTTAGAAAGA AATTAAAGAA ACCTGCGGAT ATTCCAAATA GGTTTGAAAT GAAACTTCAC
    AGAAATAACA TATTTGAAGA GTCCTATCGG AGAATCATGT CTGTGAAAAG ACCAGATGTC
    CTGAAAGCTA GGCTGTGGAT TGAGTTTGAA TCGGAGAAAG GTCTGGACTA CGGGGGCGTG
    GCCAGAGAGT GGTTCTTCCT ACTGTCCAAG GAGATGTTCA ACCCTTACTA CGGCCTCTTC
    GAGTATTCGG CAACGGACAA CTACACACTT CAGATCAATC CCAATTCAGG CCTCTGTAAT
    GAAGATCATT TGTCCTATTT CACTTTCATT GGAAGAGTTG CTGGGCTGGC AGTATTTCAT
    GGGAAACTTC TAGATGGTTT CTTCATTCGA CCGTTTTATA AAATGATGCT GGGAAAGCAG
    ATAACTCTGA ATGACATGGA ATCTGTGGAT AGCGAATATT ACAACTCTTT GAAATGGATC
    TTAGAAAATG ACCCAACTGA ACTGGACCTT ATGTTCTGCA TTGATGAAGA GAACTTTGGG
    CAGACATATC AAGTGGATCT GAAGCCCAAC GGGTCAGAAA TAATGGTAAC GAACGAAAAC
    AAGAGAGAGT ACATCGACCT CGTCATCCAG TGGAGGTTCG TGAACAGGGT CCAGAAGCAG
    ATGAACGCCT TCCTGGAGGG ATTCACGGAA CTGCTCCCTA TTGATTTGAT TAAAATCTTT
    GATGAAAATG AACTGGAGTT GCTGATGTGT GGCCTTGGCG ACGTGGATGT GAACGACTGG
    CGACAACATT CTATTTACAA GAATGGCTAC TGCCCGAACC ACCCCGTGAT CCAGTGGTTC
    TGGAAGGCTG TGCTGCTGAT GGACGCCGAG AAGCGCATCC GGTTGCTGCA GTTTGTCACA
    GGGACGTCCC GAGTACCTAT GAATGGATTT GCCGAGCTTT ATGGTTCCAA TGGTCCTCAG
    CTGTTTACAA TAGAGCAATG GGGAAGTCCT GAAAAACTGC CCAGAGCTCA CACGTGCTTT
    AATCGCCTTG ACTTACCTCC GTATGAAACC TTCGAAGATT TACGAGAGAA GCTCCTCATG
    GCTGTGGAAA ACGCCCAAGG CTTCGAGGGG GTGGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024840245.1
    CDS346..2964
    Translation

    Target ORF information:

    RefSeq Version XM_024984477.1
    Organism Bos taurus(cattle)
    Definition Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024984477.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGCCCAGTGC CCCAGCTGGG
    AGAGCACGTT CATCAACTGT CACTGGTGGT GAGGCGCCAA CGCCATCAGT GGCCTATGTA
    CACACCACGC CGGGCCTGCC CTCGGGCTGG GAAGAGAGGA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCTATCAT GCAGCTCGCA
    GAAGACGGCG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CCGCCCCGCT GGAGGGCGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GATACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAGA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA CAACACAAAG
    ACCACGACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ACATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGCTGGG AAGAAAGGAT CCACTTGGAT
    GGCCGGACGT TTTACATTGA CCACAGCAGC CAGGTGTTAT GTGGCGAGGA CGATGCCAGA
    GACTCAGTGC CCTCGTACGA TAGCAAAATT ACTCAGTGGG AAGACCCAAG ACTGCAGAAC
    CCAGCTATTA CTGGTCCGGC TGTTCCTTAC TCCAGAGAAT TTAAGCAGAA ATATGACTAC
    TTTAGAAAGA AATTAAAGAA ACCTGCGGAT ATTCCAAATA GGTTTGAAAT GAAACTTCAC
    AGAAATAACA TATTTGAAGA GTCCTATCGG AGAATCATGT CTGTGAAAAG ACCAGATGTC
    CTGAAAGCTA GGCTGTGGAT TGAGTTTGAA TCGGAGAAAG GTCTGGACTA CGGGGGCGTG
    GCCAGAGAGT GGTTCTTCCT ACTGTCCAAG GAGATGTTCA ACCCTTACTA CGGCCTCTTC
    GAGTATTCGG CAACGGACAA CTACACACTT CAGATCAATC CCAATTCAGG CCTCTGTAAT
    GAAGATCATT TGTCCTATTT CACTTTCATT GGAAGAGTTG CTGGGCTGGC AGTATTTCAT
    GGGAAACTTC TAGATGGTTT CTTCATTCGA CCGTTTTATA AAATGATGCT GGGAAAGCAG
    ATAACTCTGA ATGACATGGA ATCTGTGGAT AGCGAATATT ACAACTCTTT GAAATGGATC
    TTAGAAAATG ACCCAACTGA ACTGGACCTT ATGTTCTGCA TTGATGAAGA GAACTTTGGG
    CAGACATATC AAGTGGATCT GAAGCCCAAC GGGTCAGAAA TAATGGTAAC GAACGAAAAC
    AAGAGAGAGT ACATCGACCT CGTCATCCAG TGGAGGTTCG TGAACAGGGT CCAGAAGCAG
    ATGAACGCCT TCCTGGAGGG ATTCACGGAA CTGCTCCCTA TTGATTTGAT TAAAATCTTT
    GATGAAAATG AACTGGAGTT GCTGATGTGT GGCCTTGGCG ACGTGGATGT GAACGACTGG
    CGACAACATT CTATTTACAA GAATGGCTAC TGCCCGAACC ACCCCGTGAT CCAGTGGTTC
    TGGAAGGCTG TGCTGCTGAT GGACGCCGAG AAGCGCATCC GGTTGCTGCA GTTTGTCACA
    GGGACGTCCC GAGTACCTAT GAATGGATTT GCCGAGCTTT ATGGTTCCAA TGGTCCTCAG
    CTGTTTACAA TAGAGCAATG GGGAAGTCCT GAAAAACTGC CCAGAGCTCA CACGTGCTTT
    AATCGCCTTG ACTTACCTCC GTATGAAACC TTCGAAGATT TACGAGAGAA GCTCCTCATG
    GCTGTGGAAA ACGCCCAAGG CTTCGAGGGG GTGGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa47561
    Clone ID Related Accession (Same CDS sequence) XM_005224351.3 , XM_010819012.2 , XM_015469185.1 , XM_010799401.2 , XM_024984479.1 , XM_015460157.1 , XM_024984477.1 , XM_024984478.1 , XM_015469184.1 , XM_015460156.1 , XM_015460155.1 , XM_015469186.1 , XM_015460156.2 , XM_024984476.1 , XM_010819012.3 , XM_005199752.3 , XM_005224351.4
    Accession Version XM_024984478.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2619bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGCCCAGTGC CCCAGCTGGG
    AGAGCACGTT CATCAACTGT CACTGGTGGT GAGGCGCCAA CGCCATCAGT GGCCTATGTA
    CACACCACGC CGGGCCTGCC CTCGGGCTGG GAAGAGAGGA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCTATCAT GCAGCTCGCA
    GAAGACGGCG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CCGCCCCGCT GGAGGGCGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GATACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAGA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA CAACACAAAG
    ACCACGACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ACATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGCTGGG AAGAAAGGAT CCACTTGGAT
    GGCCGGACGT TTTACATTGA CCACAGCAGC CAGGTGTTAT GTGGCGAGGA CGATGCCAGA
    GACTCAGTGC CCTCGTACGA TAGCAAAATT ACTCAGTGGG AAGACCCAAG ACTGCAGAAC
    CCAGCTATTA CTGGTCCGGC TGTTCCTTAC TCCAGAGAAT TTAAGCAGAA ATATGACTAC
    TTTAGAAAGA AATTAAAGAA ACCTGCGGAT ATTCCAAATA GGTTTGAAAT GAAACTTCAC
    AGAAATAACA TATTTGAAGA GTCCTATCGG AGAATCATGT CTGTGAAAAG ACCAGATGTC
    CTGAAAGCTA GGCTGTGGAT TGAGTTTGAA TCGGAGAAAG GTCTGGACTA CGGGGGCGTG
    GCCAGAGAGT GGTTCTTCCT ACTGTCCAAG GAGATGTTCA ACCCTTACTA CGGCCTCTTC
    GAGTATTCGG CAACGGACAA CTACACACTT CAGATCAATC CCAATTCAGG CCTCTGTAAT
    GAAGATCATT TGTCCTATTT CACTTTCATT GGAAGAGTTG CTGGGCTGGC AGTATTTCAT
    GGGAAACTTC TAGATGGTTT CTTCATTCGA CCGTTTTATA AAATGATGCT GGGAAAGCAG
    ATAACTCTGA ATGACATGGA ATCTGTGGAT AGCGAATATT ACAACTCTTT GAAATGGATC
    TTAGAAAATG ACCCAACTGA ACTGGACCTT ATGTTCTGCA TTGATGAAGA GAACTTTGGG
    CAGACATATC AAGTGGATCT GAAGCCCAAC GGGTCAGAAA TAATGGTAAC GAACGAAAAC
    AAGAGAGAGT ACATCGACCT CGTCATCCAG TGGAGGTTCG TGAACAGGGT CCAGAAGCAG
    ATGAACGCCT TCCTGGAGGG ATTCACGGAA CTGCTCCCTA TTGATTTGAT TAAAATCTTT
    GATGAAAATG AACTGGAGTT GCTGATGTGT GGCCTTGGCG ACGTGGATGT GAACGACTGG
    CGACAACATT CTATTTACAA GAATGGCTAC TGCCCGAACC ACCCCGTGAT CCAGTGGTTC
    TGGAAGGCTG TGCTGCTGAT GGACGCCGAG AAGCGCATCC GGTTGCTGCA GTTTGTCACA
    GGGACGTCCC GAGTACCTAT GAATGGATTT GCCGAGCTTT ATGGTTCCAA TGGTCCTCAG
    CTGTTTACAA TAGAGCAATG GGGAAGTCCT GAAAAACTGC CCAGAGCTCA CACGTGCTTT
    AATCGCCTTG ACTTACCTCC GTATGAAACC TTCGAAGATT TACGAGAGAA GCTCCTCATG
    GCTGTGGAAA ACGCCCAAGG CTTCGAGGGG GTGGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024840246.1
    CDS365..2983
    Translation

    Target ORF information:

    RefSeq Version XM_024984478.1
    Organism Bos taurus(cattle)
    Definition Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024984478.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGCCCAGTGC CCCAGCTGGG
    AGAGCACGTT CATCAACTGT CACTGGTGGT GAGGCGCCAA CGCCATCAGT GGCCTATGTA
    CACACCACGC CGGGCCTGCC CTCGGGCTGG GAAGAGAGGA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCTATCAT GCAGCTCGCA
    GAAGACGGCG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CCGCCCCGCT GGAGGGCGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GATACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAGA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA CAACACAAAG
    ACCACGACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ACATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGCTGGG AAGAAAGGAT CCACTTGGAT
    GGCCGGACGT TTTACATTGA CCACAGCAGC CAGGTGTTAT GTGGCGAGGA CGATGCCAGA
    GACTCAGTGC CCTCGTACGA TAGCAAAATT ACTCAGTGGG AAGACCCAAG ACTGCAGAAC
    CCAGCTATTA CTGGTCCGGC TGTTCCTTAC TCCAGAGAAT TTAAGCAGAA ATATGACTAC
    TTTAGAAAGA AATTAAAGAA ACCTGCGGAT ATTCCAAATA GGTTTGAAAT GAAACTTCAC
    AGAAATAACA TATTTGAAGA GTCCTATCGG AGAATCATGT CTGTGAAAAG ACCAGATGTC
    CTGAAAGCTA GGCTGTGGAT TGAGTTTGAA TCGGAGAAAG GTCTGGACTA CGGGGGCGTG
    GCCAGAGAGT GGTTCTTCCT ACTGTCCAAG GAGATGTTCA ACCCTTACTA CGGCCTCTTC
    GAGTATTCGG CAACGGACAA CTACACACTT CAGATCAATC CCAATTCAGG CCTCTGTAAT
    GAAGATCATT TGTCCTATTT CACTTTCATT GGAAGAGTTG CTGGGCTGGC AGTATTTCAT
    GGGAAACTTC TAGATGGTTT CTTCATTCGA CCGTTTTATA AAATGATGCT GGGAAAGCAG
    ATAACTCTGA ATGACATGGA ATCTGTGGAT AGCGAATATT ACAACTCTTT GAAATGGATC
    TTAGAAAATG ACCCAACTGA ACTGGACCTT ATGTTCTGCA TTGATGAAGA GAACTTTGGG
    CAGACATATC AAGTGGATCT GAAGCCCAAC GGGTCAGAAA TAATGGTAAC GAACGAAAAC
    AAGAGAGAGT ACATCGACCT CGTCATCCAG TGGAGGTTCG TGAACAGGGT CCAGAAGCAG
    ATGAACGCCT TCCTGGAGGG ATTCACGGAA CTGCTCCCTA TTGATTTGAT TAAAATCTTT
    GATGAAAATG AACTGGAGTT GCTGATGTGT GGCCTTGGCG ACGTGGATGT GAACGACTGG
    CGACAACATT CTATTTACAA GAATGGCTAC TGCCCGAACC ACCCCGTGAT CCAGTGGTTC
    TGGAAGGCTG TGCTGCTGAT GGACGCCGAG AAGCGCATCC GGTTGCTGCA GTTTGTCACA
    GGGACGTCCC GAGTACCTAT GAATGGATTT GCCGAGCTTT ATGGTTCCAA TGGTCCTCAG
    CTGTTTACAA TAGAGCAATG GGGAAGTCCT GAAAAACTGC CCAGAGCTCA CACGTGCTTT
    AATCGCCTTG ACTTACCTCC GTATGAAACC TTCGAAGATT TACGAGAGAA GCTCCTCATG
    GCTGTGGAAA ACGCCCAAGG CTTCGAGGGG GTGGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa47561
    Clone ID Related Accession (Same CDS sequence) XM_005224351.3 , XM_010819012.2 , XM_015469185.1 , XM_010799401.2 , XM_024984479.1 , XM_015460157.1 , XM_024984477.1 , XM_024984478.1 , XM_015469184.1 , XM_015460156.1 , XM_015460155.1 , XM_015469186.1 , XM_015460156.2 , XM_024984476.1 , XM_010819012.3 , XM_005199752.3 , XM_005224351.4
    Accession Version XM_015469184.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2619bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646636.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGCCCAGTGC CCCAGCTGGG
    AGAGCACGTT CATCAACTGT CACTGGTGGT GAGGCGCCAA CGCCATCAGT GGCCTATGTA
    CACACCACGC CGGGCCTGCC CTCGGGCTGG GAAGAGAGGA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCTATCAT GCAGCTCGCA
    GAAGACGGCG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CCGCCCCGCT GGAGGGCGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GATACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAGA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA CAACACAAAG
    ACCACGACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ACATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGCTGGG AAGAAAGGAT CCACTTGGAT
    GGCCGGACGT TTTACATTGA CCACAGCAGC CAGGTGTTAT GTGGCGAGGA CGATGCCAGA
    GACTCAGTGC CCTCGTACGA TAGCAAAATT ACTCAGTGGG AAGACCCAAG ACTGCAGAAC
    CCAGCTATTA CTGGTCCGGC TGTTCCTTAC TCCAGAGAAT TTAAGCAGAA ATATGACTAC
    TTTAGAAAGA AATTAAAGAA ACCTGCGGAT ATTCCAAATA GGTTTGAAAT GAAACTTCAC
    AGAAATAACA TATTTGAAGA GTCCTATCGG AGAATCATGT CTGTGAAAAG ACCAGATGTC
    CTGAAAGCTA GGCTGTGGAT TGAGTTTGAA TCGGAGAAAG GTCTGGACTA CGGGGGCGTG
    GCCAGAGAGT GGTTCTTCCT ACTGTCCAAG GAGATGTTCA ACCCTTACTA CGGCCTCTTC
    GAGTATTCGG CAACGGACAA CTACACACTT CAGATCAATC CCAATTCAGG CCTCTGTAAT
    GAAGATCATT TGTCCTATTT CACTTTCATT GGAAGAGTTG CTGGGCTGGC AGTATTTCAT
    GGGAAACTTC TAGATGGTTT CTTCATTCGA CCGTTTTATA AAATGATGCT GGGAAAGCAG
    ATAACTCTGA ATGACATGGA ATCTGTGGAT AGCGAATATT ACAACTCTTT GAAATGGATC
    TTAGAAAATG ACCCAACTGA ACTGGACCTT ATGTTCTGCA TTGATGAAGA GAACTTTGGG
    CAGACATATC AAGTGGATCT GAAGCCCAAC GGGTCAGAAA TAATGGTAAC GAACGAAAAC
    AAGAGAGAGT ACATCGACCT CGTCATCCAG TGGAGGTTCG TGAACAGGGT CCAGAAGCAG
    ATGAACGCCT TCCTGGAGGG ATTCACGGAA CTGCTCCCTA TTGATTTGAT TAAAATCTTT
    GATGAAAATG AACTGGAGTT GCTGATGTGT GGCCTTGGCG ACGTGGATGT GAACGACTGG
    CGACAACATT CTATTTACAA GAATGGCTAC TGCCCGAACC ACCCCGTGAT CCAGTGGTTC
    TGGAAGGCTG TGCTGCTGAT GGACGCCGAG AAGCGCATCC GGTTGCTGCA GTTTGTCACA
    GGGACGTCCC GAGTACCTAT GAATGGATTT GCCGAGCTTT ATGGTTCCAA TGGTCCTCAG
    CTGTTTACAA TAGAGCAATG GGGAAGTCCT GAAAAACTGC CCAGAGCTCA CACGTGCTTT
    AATCGCCTTG ACTTACCTCC GTATGAAACC TTCGAAGATT TACGAGAGAA GCTCCTCATG
    GCTGTGGAAA ACGCCCAAGG CTTCGAGGGG GTGGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015324670.1
    CDS377..2995
    Translation

    Target ORF information:

    RefSeq Version XM_015469184.1
    Organism Bos taurus(cattle)
    Definition Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015469184.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGCCCAGTGC CCCAGCTGGG
    AGAGCACGTT CATCAACTGT CACTGGTGGT GAGGCGCCAA CGCCATCAGT GGCCTATGTA
    CACACCACGC CGGGCCTGCC CTCGGGCTGG GAAGAGAGGA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCTATCAT GCAGCTCGCA
    GAAGACGGCG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CCGCCCCGCT GGAGGGCGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GATACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAGA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA CAACACAAAG
    ACCACGACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ACATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGCTGGG AAGAAAGGAT CCACTTGGAT
    GGCCGGACGT TTTACATTGA CCACAGCAGC CAGGTGTTAT GTGGCGAGGA CGATGCCAGA
    GACTCAGTGC CCTCGTACGA TAGCAAAATT ACTCAGTGGG AAGACCCAAG ACTGCAGAAC
    CCAGCTATTA CTGGTCCGGC TGTTCCTTAC TCCAGAGAAT TTAAGCAGAA ATATGACTAC
    TTTAGAAAGA AATTAAAGAA ACCTGCGGAT ATTCCAAATA GGTTTGAAAT GAAACTTCAC
    AGAAATAACA TATTTGAAGA GTCCTATCGG AGAATCATGT CTGTGAAAAG ACCAGATGTC
    CTGAAAGCTA GGCTGTGGAT TGAGTTTGAA TCGGAGAAAG GTCTGGACTA CGGGGGCGTG
    GCCAGAGAGT GGTTCTTCCT ACTGTCCAAG GAGATGTTCA ACCCTTACTA CGGCCTCTTC
    GAGTATTCGG CAACGGACAA CTACACACTT CAGATCAATC CCAATTCAGG CCTCTGTAAT
    GAAGATCATT TGTCCTATTT CACTTTCATT GGAAGAGTTG CTGGGCTGGC AGTATTTCAT
    GGGAAACTTC TAGATGGTTT CTTCATTCGA CCGTTTTATA AAATGATGCT GGGAAAGCAG
    ATAACTCTGA ATGACATGGA ATCTGTGGAT AGCGAATATT ACAACTCTTT GAAATGGATC
    TTAGAAAATG ACCCAACTGA ACTGGACCTT ATGTTCTGCA TTGATGAAGA GAACTTTGGG
    CAGACATATC AAGTGGATCT GAAGCCCAAC GGGTCAGAAA TAATGGTAAC GAACGAAAAC
    AAGAGAGAGT ACATCGACCT CGTCATCCAG TGGAGGTTCG TGAACAGGGT CCAGAAGCAG
    ATGAACGCCT TCCTGGAGGG ATTCACGGAA CTGCTCCCTA TTGATTTGAT TAAAATCTTT
    GATGAAAATG AACTGGAGTT GCTGATGTGT GGCCTTGGCG ACGTGGATGT GAACGACTGG
    CGACAACATT CTATTTACAA GAATGGCTAC TGCCCGAACC ACCCCGTGAT CCAGTGGTTC
    TGGAAGGCTG TGCTGCTGAT GGACGCCGAG AAGCGCATCC GGTTGCTGCA GTTTGTCACA
    GGGACGTCCC GAGTACCTAT GAATGGATTT GCCGAGCTTT ATGGTTCCAA TGGTCCTCAG
    CTGTTTACAA TAGAGCAATG GGGAAGTCCT GAAAAACTGC CCAGAGCTCA CACGTGCTTT
    AATCGCCTTG ACTTACCTCC GTATGAAACC TTCGAAGATT TACGAGAGAA GCTCCTCATG
    GCTGTGGAAA ACGCCCAAGG CTTCGAGGGG GTGGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa47561
    Clone ID Related Accession (Same CDS sequence) XM_005224351.3 , XM_010819012.2 , XM_015469185.1 , XM_010799401.2 , XM_024984479.1 , XM_015460157.1 , XM_024984477.1 , XM_024984478.1 , XM_015469184.1 , XM_015460156.1 , XM_015460155.1 , XM_015469186.1 , XM_015460156.2 , XM_024984476.1 , XM_010819012.3 , XM_005199752.3 , XM_005224351.4
    Accession Version XM_015460156.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2619bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104569.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGCCCAGTGC CCCAGCTGGG
    AGAGCACGTT CATCAACTGT CACTGGTGGT GAGGCGCCAA CGCCATCAGT GGCCTATGTA
    CACACCACGC CGGGCCTGCC CTCGGGCTGG GAAGAGAGGA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCTATCAT GCAGCTCGCA
    GAAGACGGCG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CCGCCCCGCT GGAGGGCGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GATACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAGA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA CAACACAAAG
    ACCACGACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ACATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGCTGGG AAGAAAGGAT CCACTTGGAT
    GGCCGGACGT TTTACATTGA CCACAGCAGC CAGGTGTTAT GTGGCGAGGA CGATGCCAGA
    GACTCAGTGC CCTCGTACGA TAGCAAAATT ACTCAGTGGG AAGACCCAAG ACTGCAGAAC
    CCAGCTATTA CTGGTCCGGC TGTTCCTTAC TCCAGAGAAT TTAAGCAGAA ATATGACTAC
    TTTAGAAAGA AATTAAAGAA ACCTGCGGAT ATTCCAAATA GGTTTGAAAT GAAACTTCAC
    AGAAATAACA TATTTGAAGA GTCCTATCGG AGAATCATGT CTGTGAAAAG ACCAGATGTC
    CTGAAAGCTA GGCTGTGGAT TGAGTTTGAA TCGGAGAAAG GTCTGGACTA CGGGGGCGTG
    GCCAGAGAGT GGTTCTTCCT ACTGTCCAAG GAGATGTTCA ACCCTTACTA CGGCCTCTTC
    GAGTATTCGG CAACGGACAA CTACACACTT CAGATCAATC CCAATTCAGG CCTCTGTAAT
    GAAGATCATT TGTCCTATTT CACTTTCATT GGAAGAGTTG CTGGGCTGGC AGTATTTCAT
    GGGAAACTTC TAGATGGTTT CTTCATTCGA CCGTTTTATA AAATGATGCT GGGAAAGCAG
    ATAACTCTGA ATGACATGGA ATCTGTGGAT AGCGAATATT ACAACTCTTT GAAATGGATC
    TTAGAAAATG ACCCAACTGA ACTGGACCTT ATGTTCTGCA TTGATGAAGA GAACTTTGGG
    CAGACATATC AAGTGGATCT GAAGCCCAAC GGGTCAGAAA TAATGGTAAC GAACGAAAAC
    AAGAGAGAGT ACATCGACCT CGTCATCCAG TGGAGGTTCG TGAACAGGGT CCAGAAGCAG
    ATGAACGCCT TCCTGGAGGG ATTCACGGAA CTGCTCCCTA TTGATTTGAT TAAAATCTTT
    GATGAAAATG AACTGGAGTT GCTGATGTGT GGCCTTGGCG ACGTGGATGT GAACGACTGG
    CGACAACATT CTATTTACAA GAATGGCTAC TGCCCGAACC ACCCCGTGAT CCAGTGGTTC
    TGGAAGGCTG TGCTGCTGAT GGACGCCGAG AAGCGCATCC GGTTGCTGCA GTTTGTCACA
    GGGACGTCCC GAGTACCTAT GAATGGATTT GCCGAGCTTT ATGGTTCCAA TGGTCCTCAG
    CTGTTTACAA TAGAGCAATG GGGAAGTCCT GAAAAACTGC CCAGAGCTCA CACGTGCTTT
    AATCGCCTTG ACTTACCTCC GTATGAAACC TTCGAAGATT TACGAGAGAA GCTCCTCATG
    GCTGTGGAAA ACGCCCAAGG CTTCGAGGGG GTGGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015315642.1
    CDS1086..3704
    Translation

    Target ORF information:

    RefSeq Version XM_015460156.1
    Organism Bos taurus(cattle)
    Definition Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015460156.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGCCCAGTGC CCCAGCTGGG
    AGAGCACGTT CATCAACTGT CACTGGTGGT GAGGCGCCAA CGCCATCAGT GGCCTATGTA
    CACACCACGC CGGGCCTGCC CTCGGGCTGG GAAGAGAGGA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCTATCAT GCAGCTCGCA
    GAAGACGGCG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CCGCCCCGCT GGAGGGCGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GATACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAGA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA CAACACAAAG
    ACCACGACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ACATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGCTGGG AAGAAAGGAT CCACTTGGAT
    GGCCGGACGT TTTACATTGA CCACAGCAGC CAGGTGTTAT GTGGCGAGGA CGATGCCAGA
    GACTCAGTGC CCTCGTACGA TAGCAAAATT ACTCAGTGGG AAGACCCAAG ACTGCAGAAC
    CCAGCTATTA CTGGTCCGGC TGTTCCTTAC TCCAGAGAAT TTAAGCAGAA ATATGACTAC
    TTTAGAAAGA AATTAAAGAA ACCTGCGGAT ATTCCAAATA GGTTTGAAAT GAAACTTCAC
    AGAAATAACA TATTTGAAGA GTCCTATCGG AGAATCATGT CTGTGAAAAG ACCAGATGTC
    CTGAAAGCTA GGCTGTGGAT TGAGTTTGAA TCGGAGAAAG GTCTGGACTA CGGGGGCGTG
    GCCAGAGAGT GGTTCTTCCT ACTGTCCAAG GAGATGTTCA ACCCTTACTA CGGCCTCTTC
    GAGTATTCGG CAACGGACAA CTACACACTT CAGATCAATC CCAATTCAGG CCTCTGTAAT
    GAAGATCATT TGTCCTATTT CACTTTCATT GGAAGAGTTG CTGGGCTGGC AGTATTTCAT
    GGGAAACTTC TAGATGGTTT CTTCATTCGA CCGTTTTATA AAATGATGCT GGGAAAGCAG
    ATAACTCTGA ATGACATGGA ATCTGTGGAT AGCGAATATT ACAACTCTTT GAAATGGATC
    TTAGAAAATG ACCCAACTGA ACTGGACCTT ATGTTCTGCA TTGATGAAGA GAACTTTGGG
    CAGACATATC AAGTGGATCT GAAGCCCAAC GGGTCAGAAA TAATGGTAAC GAACGAAAAC
    AAGAGAGAGT ACATCGACCT CGTCATCCAG TGGAGGTTCG TGAACAGGGT CCAGAAGCAG
    ATGAACGCCT TCCTGGAGGG ATTCACGGAA CTGCTCCCTA TTGATTTGAT TAAAATCTTT
    GATGAAAATG AACTGGAGTT GCTGATGTGT GGCCTTGGCG ACGTGGATGT GAACGACTGG
    CGACAACATT CTATTTACAA GAATGGCTAC TGCCCGAACC ACCCCGTGAT CCAGTGGTTC
    TGGAAGGCTG TGCTGCTGAT GGACGCCGAG AAGCGCATCC GGTTGCTGCA GTTTGTCACA
    GGGACGTCCC GAGTACCTAT GAATGGATTT GCCGAGCTTT ATGGTTCCAA TGGTCCTCAG
    CTGTTTACAA TAGAGCAATG GGGAAGTCCT GAAAAACTGC CCAGAGCTCA CACGTGCTTT
    AATCGCCTTG ACTTACCTCC GTATGAAACC TTCGAAGATT TACGAGAGAA GCTCCTCATG
    GCTGTGGAAA ACGCCCAAGG CTTCGAGGGG GTGGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa47561
    Clone ID Related Accession (Same CDS sequence) XM_005224351.3 , XM_010819012.2 , XM_015469185.1 , XM_010799401.2 , XM_024984479.1 , XM_015460157.1 , XM_024984477.1 , XM_024984478.1 , XM_015469184.1 , XM_015460156.1 , XM_015460155.1 , XM_015469186.1 , XM_015460156.2 , XM_024984476.1 , XM_010819012.3 , XM_005199752.3 , XM_005224351.4
    Accession Version XM_015460155.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2619bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104569.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGCCCAGTGC CCCAGCTGGG
    AGAGCACGTT CATCAACTGT CACTGGTGGT GAGGCGCCAA CGCCATCAGT GGCCTATGTA
    CACACCACGC CGGGCCTGCC CTCGGGCTGG GAAGAGAGGA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCTATCAT GCAGCTCGCA
    GAAGACGGCG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CCGCCCCGCT GGAGGGCGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GATACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAGA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA CAACACAAAG
    ACCACGACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ACATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGCTGGG AAGAAAGGAT CCACTTGGAT
    GGCCGGACGT TTTACATTGA CCACAGCAGC CAGGTGTTAT GTGGCGAGGA CGATGCCAGA
    GACTCAGTGC CCTCGTACGA TAGCAAAATT ACTCAGTGGG AAGACCCAAG ACTGCAGAAC
    CCAGCTATTA CTGGTCCGGC TGTTCCTTAC TCCAGAGAAT TTAAGCAGAA ATATGACTAC
    TTTAGAAAGA AATTAAAGAA ACCTGCGGAT ATTCCAAATA GGTTTGAAAT GAAACTTCAC
    AGAAATAACA TATTTGAAGA GTCCTATCGG AGAATCATGT CTGTGAAAAG ACCAGATGTC
    CTGAAAGCTA GGCTGTGGAT TGAGTTTGAA TCGGAGAAAG GTCTGGACTA CGGGGGCGTG
    GCCAGAGAGT GGTTCTTCCT ACTGTCCAAG GAGATGTTCA ACCCTTACTA CGGCCTCTTC
    GAGTATTCGG CAACGGACAA CTACACACTT CAGATCAATC CCAATTCAGG CCTCTGTAAT
    GAAGATCATT TGTCCTATTT CACTTTCATT GGAAGAGTTG CTGGGCTGGC AGTATTTCAT
    GGGAAACTTC TAGATGGTTT CTTCATTCGA CCGTTTTATA AAATGATGCT GGGAAAGCAG
    ATAACTCTGA ATGACATGGA ATCTGTGGAT AGCGAATATT ACAACTCTTT GAAATGGATC
    TTAGAAAATG ACCCAACTGA ACTGGACCTT ATGTTCTGCA TTGATGAAGA GAACTTTGGG
    CAGACATATC AAGTGGATCT GAAGCCCAAC GGGTCAGAAA TAATGGTAAC GAACGAAAAC
    AAGAGAGAGT ACATCGACCT CGTCATCCAG TGGAGGTTCG TGAACAGGGT CCAGAAGCAG
    ATGAACGCCT TCCTGGAGGG ATTCACGGAA CTGCTCCCTA TTGATTTGAT TAAAATCTTT
    GATGAAAATG AACTGGAGTT GCTGATGTGT GGCCTTGGCG ACGTGGATGT GAACGACTGG
    CGACAACATT CTATTTACAA GAATGGCTAC TGCCCGAACC ACCCCGTGAT CCAGTGGTTC
    TGGAAGGCTG TGCTGCTGAT GGACGCCGAG AAGCGCATCC GGTTGCTGCA GTTTGTCACA
    GGGACGTCCC GAGTACCTAT GAATGGATTT GCCGAGCTTT ATGGTTCCAA TGGTCCTCAG
    CTGTTTACAA TAGAGCAATG GGGAAGTCCT GAAAAACTGC CCAGAGCTCA CACGTGCTTT
    AATCGCCTTG ACTTACCTCC GTATGAAACC TTCGAAGATT TACGAGAGAA GCTCCTCATG
    GCTGTGGAAA ACGCCCAAGG CTTCGAGGGG GTGGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015315641.1
    CDS377..2995
    Translation

    Target ORF information:

    RefSeq Version XM_015460155.1
    Organism Bos taurus(cattle)
    Definition Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015460155.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGCCCAGTGC CCCAGCTGGG
    AGAGCACGTT CATCAACTGT CACTGGTGGT GAGGCGCCAA CGCCATCAGT GGCCTATGTA
    CACACCACGC CGGGCCTGCC CTCGGGCTGG GAAGAGAGGA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCTATCAT GCAGCTCGCA
    GAAGACGGCG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CCGCCCCGCT GGAGGGCGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GATACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAGA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA CAACACAAAG
    ACCACGACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ACATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGCTGGG AAGAAAGGAT CCACTTGGAT
    GGCCGGACGT TTTACATTGA CCACAGCAGC CAGGTGTTAT GTGGCGAGGA CGATGCCAGA
    GACTCAGTGC CCTCGTACGA TAGCAAAATT ACTCAGTGGG AAGACCCAAG ACTGCAGAAC
    CCAGCTATTA CTGGTCCGGC TGTTCCTTAC TCCAGAGAAT TTAAGCAGAA ATATGACTAC
    TTTAGAAAGA AATTAAAGAA ACCTGCGGAT ATTCCAAATA GGTTTGAAAT GAAACTTCAC
    AGAAATAACA TATTTGAAGA GTCCTATCGG AGAATCATGT CTGTGAAAAG ACCAGATGTC
    CTGAAAGCTA GGCTGTGGAT TGAGTTTGAA TCGGAGAAAG GTCTGGACTA CGGGGGCGTG
    GCCAGAGAGT GGTTCTTCCT ACTGTCCAAG GAGATGTTCA ACCCTTACTA CGGCCTCTTC
    GAGTATTCGG CAACGGACAA CTACACACTT CAGATCAATC CCAATTCAGG CCTCTGTAAT
    GAAGATCATT TGTCCTATTT CACTTTCATT GGAAGAGTTG CTGGGCTGGC AGTATTTCAT
    GGGAAACTTC TAGATGGTTT CTTCATTCGA CCGTTTTATA AAATGATGCT GGGAAAGCAG
    ATAACTCTGA ATGACATGGA ATCTGTGGAT AGCGAATATT ACAACTCTTT GAAATGGATC
    TTAGAAAATG ACCCAACTGA ACTGGACCTT ATGTTCTGCA TTGATGAAGA GAACTTTGGG
    CAGACATATC AAGTGGATCT GAAGCCCAAC GGGTCAGAAA TAATGGTAAC GAACGAAAAC
    AAGAGAGAGT ACATCGACCT CGTCATCCAG TGGAGGTTCG TGAACAGGGT CCAGAAGCAG
    ATGAACGCCT TCCTGGAGGG ATTCACGGAA CTGCTCCCTA TTGATTTGAT TAAAATCTTT
    GATGAAAATG AACTGGAGTT GCTGATGTGT GGCCTTGGCG ACGTGGATGT GAACGACTGG
    CGACAACATT CTATTTACAA GAATGGCTAC TGCCCGAACC ACCCCGTGAT CCAGTGGTTC
    TGGAAGGCTG TGCTGCTGAT GGACGCCGAG AAGCGCATCC GGTTGCTGCA GTTTGTCACA
    GGGACGTCCC GAGTACCTAT GAATGGATTT GCCGAGCTTT ATGGTTCCAA TGGTCCTCAG
    CTGTTTACAA TAGAGCAATG GGGAAGTCCT GAAAAACTGC CCAGAGCTCA CACGTGCTTT
    AATCGCCTTG ACTTACCTCC GTATGAAACC TTCGAAGATT TACGAGAGAA GCTCCTCATG
    GCTGTGGAAA ACGCCCAAGG CTTCGAGGGG GTGGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa47561
    Clone ID Related Accession (Same CDS sequence) XM_005224351.3 , XM_010819012.2 , XM_015469185.1 , XM_010799401.2 , XM_024984479.1 , XM_015460157.1 , XM_024984477.1 , XM_024984478.1 , XM_015469184.1 , XM_015460156.1 , XM_015460155.1 , XM_015469186.1 , XM_015460156.2 , XM_024984476.1 , XM_010819012.3 , XM_005199752.3 , XM_005224351.4
    Accession Version XM_015469186.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2619bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646636.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGCCCAGTGC CCCAGCTGGG
    AGAGCACGTT CATCAACTGT CACTGGTGGT GAGGCGCCAA CGCCATCAGT GGCCTATGTA
    CACACCACGC CGGGCCTGCC CTCGGGCTGG GAAGAGAGGA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCTATCAT GCAGCTCGCA
    GAAGACGGCG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CCGCCCCGCT GGAGGGCGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GATACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAGA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA CAACACAAAG
    ACCACGACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ACATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGCTGGG AAGAAAGGAT CCACTTGGAT
    GGCCGGACGT TTTACATTGA CCACAGCAGC CAGGTGTTAT GTGGCGAGGA CGATGCCAGA
    GACTCAGTGC CCTCGTACGA TAGCAAAATT ACTCAGTGGG AAGACCCAAG ACTGCAGAAC
    CCAGCTATTA CTGGTCCGGC TGTTCCTTAC TCCAGAGAAT TTAAGCAGAA ATATGACTAC
    TTTAGAAAGA AATTAAAGAA ACCTGCGGAT ATTCCAAATA GGTTTGAAAT GAAACTTCAC
    AGAAATAACA TATTTGAAGA GTCCTATCGG AGAATCATGT CTGTGAAAAG ACCAGATGTC
    CTGAAAGCTA GGCTGTGGAT TGAGTTTGAA TCGGAGAAAG GTCTGGACTA CGGGGGCGTG
    GCCAGAGAGT GGTTCTTCCT ACTGTCCAAG GAGATGTTCA ACCCTTACTA CGGCCTCTTC
    GAGTATTCGG CAACGGACAA CTACACACTT CAGATCAATC CCAATTCAGG CCTCTGTAAT
    GAAGATCATT TGTCCTATTT CACTTTCATT GGAAGAGTTG CTGGGCTGGC AGTATTTCAT
    GGGAAACTTC TAGATGGTTT CTTCATTCGA CCGTTTTATA AAATGATGCT GGGAAAGCAG
    ATAACTCTGA ATGACATGGA ATCTGTGGAT AGCGAATATT ACAACTCTTT GAAATGGATC
    TTAGAAAATG ACCCAACTGA ACTGGACCTT ATGTTCTGCA TTGATGAAGA GAACTTTGGG
    CAGACATATC AAGTGGATCT GAAGCCCAAC GGGTCAGAAA TAATGGTAAC GAACGAAAAC
    AAGAGAGAGT ACATCGACCT CGTCATCCAG TGGAGGTTCG TGAACAGGGT CCAGAAGCAG
    ATGAACGCCT TCCTGGAGGG ATTCACGGAA CTGCTCCCTA TTGATTTGAT TAAAATCTTT
    GATGAAAATG AACTGGAGTT GCTGATGTGT GGCCTTGGCG ACGTGGATGT GAACGACTGG
    CGACAACATT CTATTTACAA GAATGGCTAC TGCCCGAACC ACCCCGTGAT CCAGTGGTTC
    TGGAAGGCTG TGCTGCTGAT GGACGCCGAG AAGCGCATCC GGTTGCTGCA GTTTGTCACA
    GGGACGTCCC GAGTACCTAT GAATGGATTT GCCGAGCTTT ATGGTTCCAA TGGTCCTCAG
    CTGTTTACAA TAGAGCAATG GGGAAGTCCT GAAAAACTGC CCAGAGCTCA CACGTGCTTT
    AATCGCCTTG ACTTACCTCC GTATGAAACC TTCGAAGATT TACGAGAGAA GCTCCTCATG
    GCTGTGGAAA ACGCCCAAGG CTTCGAGGGG GTGGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015324672.1
    CDS392..3010
    Translation

    Target ORF information:

    RefSeq Version XM_015469186.1
    Organism Bos taurus(cattle)
    Definition Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015469186.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGCCCAGTGC CCCAGCTGGG
    AGAGCACGTT CATCAACTGT CACTGGTGGT GAGGCGCCAA CGCCATCAGT GGCCTATGTA
    CACACCACGC CGGGCCTGCC CTCGGGCTGG GAAGAGAGGA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCTATCAT GCAGCTCGCA
    GAAGACGGCG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CCGCCCCGCT GGAGGGCGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GATACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAGA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA CAACACAAAG
    ACCACGACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ACATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGCTGGG AAGAAAGGAT CCACTTGGAT
    GGCCGGACGT TTTACATTGA CCACAGCAGC CAGGTGTTAT GTGGCGAGGA CGATGCCAGA
    GACTCAGTGC CCTCGTACGA TAGCAAAATT ACTCAGTGGG AAGACCCAAG ACTGCAGAAC
    CCAGCTATTA CTGGTCCGGC TGTTCCTTAC TCCAGAGAAT TTAAGCAGAA ATATGACTAC
    TTTAGAAAGA AATTAAAGAA ACCTGCGGAT ATTCCAAATA GGTTTGAAAT GAAACTTCAC
    AGAAATAACA TATTTGAAGA GTCCTATCGG AGAATCATGT CTGTGAAAAG ACCAGATGTC
    CTGAAAGCTA GGCTGTGGAT TGAGTTTGAA TCGGAGAAAG GTCTGGACTA CGGGGGCGTG
    GCCAGAGAGT GGTTCTTCCT ACTGTCCAAG GAGATGTTCA ACCCTTACTA CGGCCTCTTC
    GAGTATTCGG CAACGGACAA CTACACACTT CAGATCAATC CCAATTCAGG CCTCTGTAAT
    GAAGATCATT TGTCCTATTT CACTTTCATT GGAAGAGTTG CTGGGCTGGC AGTATTTCAT
    GGGAAACTTC TAGATGGTTT CTTCATTCGA CCGTTTTATA AAATGATGCT GGGAAAGCAG
    ATAACTCTGA ATGACATGGA ATCTGTGGAT AGCGAATATT ACAACTCTTT GAAATGGATC
    TTAGAAAATG ACCCAACTGA ACTGGACCTT ATGTTCTGCA TTGATGAAGA GAACTTTGGG
    CAGACATATC AAGTGGATCT GAAGCCCAAC GGGTCAGAAA TAATGGTAAC GAACGAAAAC
    AAGAGAGAGT ACATCGACCT CGTCATCCAG TGGAGGTTCG TGAACAGGGT CCAGAAGCAG
    ATGAACGCCT TCCTGGAGGG ATTCACGGAA CTGCTCCCTA TTGATTTGAT TAAAATCTTT
    GATGAAAATG AACTGGAGTT GCTGATGTGT GGCCTTGGCG ACGTGGATGT GAACGACTGG
    CGACAACATT CTATTTACAA GAATGGCTAC TGCCCGAACC ACCCCGTGAT CCAGTGGTTC
    TGGAAGGCTG TGCTGCTGAT GGACGCCGAG AAGCGCATCC GGTTGCTGCA GTTTGTCACA
    GGGACGTCCC GAGTACCTAT GAATGGATTT GCCGAGCTTT ATGGTTCCAA TGGTCCTCAG
    CTGTTTACAA TAGAGCAATG GGGAAGTCCT GAAAAACTGC CCAGAGCTCA CACGTGCTTT
    AATCGCCTTG ACTTACCTCC GTATGAAACC TTCGAAGATT TACGAGAGAA GCTCCTCATG
    GCTGTGGAAA ACGCCCAAGG CTTCGAGGGG GTGGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa47561
    Clone ID Related Accession (Same CDS sequence) XM_005224351.3 , XM_010819012.2 , XM_015469185.1 , XM_010799401.2 , XM_024984479.1 , XM_015460157.1 , XM_024984477.1 , XM_024984478.1 , XM_015469184.1 , XM_015460156.1 , XM_015460155.1 , XM_015469186.1 , XM_015460156.2 , XM_024984476.1 , XM_010819012.3 , XM_005199752.3 , XM_005224351.4
    Accession Version XM_015460156.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2619bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015460156.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGCCCAGTGC CCCAGCTGGG
    AGAGCACGTT CATCAACTGT CACTGGTGGT GAGGCGCCAA CGCCATCAGT GGCCTATGTA
    CACACCACGC CGGGCCTGCC CTCGGGCTGG GAAGAGAGGA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCTATCAT GCAGCTCGCA
    GAAGACGGCG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CCGCCCCGCT GGAGGGCGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GATACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAGA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA CAACACAAAG
    ACCACGACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ACATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGCTGGG AAGAAAGGAT CCACTTGGAT
    GGCCGGACGT TTTACATTGA CCACAGCAGC CAGGTGTTAT GTGGCGAGGA CGATGCCAGA
    GACTCAGTGC CCTCGTACGA TAGCAAAATT ACTCAGTGGG AAGACCCAAG ACTGCAGAAC
    CCAGCTATTA CTGGTCCGGC TGTTCCTTAC TCCAGAGAAT TTAAGCAGAA ATATGACTAC
    TTTAGAAAGA AATTAAAGAA ACCTGCGGAT ATTCCAAATA GGTTTGAAAT GAAACTTCAC
    AGAAATAACA TATTTGAAGA GTCCTATCGG AGAATCATGT CTGTGAAAAG ACCAGATGTC
    CTGAAAGCTA GGCTGTGGAT TGAGTTTGAA TCGGAGAAAG GTCTGGACTA CGGGGGCGTG
    GCCAGAGAGT GGTTCTTCCT ACTGTCCAAG GAGATGTTCA ACCCTTACTA CGGCCTCTTC
    GAGTATTCGG CAACGGACAA CTACACACTT CAGATCAATC CCAATTCAGG CCTCTGTAAT
    GAAGATCATT TGTCCTATTT CACTTTCATT GGAAGAGTTG CTGGGCTGGC AGTATTTCAT
    GGGAAACTTC TAGATGGTTT CTTCATTCGA CCGTTTTATA AAATGATGCT GGGAAAGCAG
    ATAACTCTGA ATGACATGGA ATCTGTGGAT AGCGAATATT ACAACTCTTT GAAATGGATC
    TTAGAAAATG ACCCAACTGA ACTGGACCTT ATGTTCTGCA TTGATGAAGA GAACTTTGGG
    CAGACATATC AAGTGGATCT GAAGCCCAAC GGGTCAGAAA TAATGGTAAC GAACGAAAAC
    AAGAGAGAGT ACATCGACCT CGTCATCCAG TGGAGGTTCG TGAACAGGGT CCAGAAGCAG
    ATGAACGCCT TCCTGGAGGG ATTCACGGAA CTGCTCCCTA TTGATTTGAT TAAAATCTTT
    GATGAAAATG AACTGGAGTT GCTGATGTGT GGCCTTGGCG ACGTGGATGT GAACGACTGG
    CGACAACATT CTATTTACAA GAATGGCTAC TGCCCGAACC ACCCCGTGAT CCAGTGGTTC
    TGGAAGGCTG TGCTGCTGAT GGACGCCGAG AAGCGCATCC GGTTGCTGCA GTTTGTCACA
    GGGACGTCCC GAGTACCTAT GAATGGATTT GCCGAGCTTT ATGGTTCCAA TGGTCCTCAG
    CTGTTTACAA TAGAGCAATG GGGAAGTCCT GAAAAACTGC CCAGAGCTCA CACGTGCTTT
    AATCGCCTTG ACTTACCTCC GTATGAAACC TTCGAAGATT TACGAGAGAA GCTCCTCATG
    GCTGTGGAAA ACGCCCAAGG CTTCGAGGGG GTGGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015315642.1
    CDS437..3055
    Translation

    Target ORF information:

    RefSeq Version XM_015460156.2
    Organism Bos taurus(cattle)
    Definition Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015460156.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGCCCAGTGC CCCAGCTGGG
    AGAGCACGTT CATCAACTGT CACTGGTGGT GAGGCGCCAA CGCCATCAGT GGCCTATGTA
    CACACCACGC CGGGCCTGCC CTCGGGCTGG GAAGAGAGGA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCTATCAT GCAGCTCGCA
    GAAGACGGCG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CCGCCCCGCT GGAGGGCGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GATACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAGA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA CAACACAAAG
    ACCACGACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ACATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGCTGGG AAGAAAGGAT CCACTTGGAT
    GGCCGGACGT TTTACATTGA CCACAGCAGC CAGGTGTTAT GTGGCGAGGA CGATGCCAGA
    GACTCAGTGC CCTCGTACGA TAGCAAAATT ACTCAGTGGG AAGACCCAAG ACTGCAGAAC
    CCAGCTATTA CTGGTCCGGC TGTTCCTTAC TCCAGAGAAT TTAAGCAGAA ATATGACTAC
    TTTAGAAAGA AATTAAAGAA ACCTGCGGAT ATTCCAAATA GGTTTGAAAT GAAACTTCAC
    AGAAATAACA TATTTGAAGA GTCCTATCGG AGAATCATGT CTGTGAAAAG ACCAGATGTC
    CTGAAAGCTA GGCTGTGGAT TGAGTTTGAA TCGGAGAAAG GTCTGGACTA CGGGGGCGTG
    GCCAGAGAGT GGTTCTTCCT ACTGTCCAAG GAGATGTTCA ACCCTTACTA CGGCCTCTTC
    GAGTATTCGG CAACGGACAA CTACACACTT CAGATCAATC CCAATTCAGG CCTCTGTAAT
    GAAGATCATT TGTCCTATTT CACTTTCATT GGAAGAGTTG CTGGGCTGGC AGTATTTCAT
    GGGAAACTTC TAGATGGTTT CTTCATTCGA CCGTTTTATA AAATGATGCT GGGAAAGCAG
    ATAACTCTGA ATGACATGGA ATCTGTGGAT AGCGAATATT ACAACTCTTT GAAATGGATC
    TTAGAAAATG ACCCAACTGA ACTGGACCTT ATGTTCTGCA TTGATGAAGA GAACTTTGGG
    CAGACATATC AAGTGGATCT GAAGCCCAAC GGGTCAGAAA TAATGGTAAC GAACGAAAAC
    AAGAGAGAGT ACATCGACCT CGTCATCCAG TGGAGGTTCG TGAACAGGGT CCAGAAGCAG
    ATGAACGCCT TCCTGGAGGG ATTCACGGAA CTGCTCCCTA TTGATTTGAT TAAAATCTTT
    GATGAAAATG AACTGGAGTT GCTGATGTGT GGCCTTGGCG ACGTGGATGT GAACGACTGG
    CGACAACATT CTATTTACAA GAATGGCTAC TGCCCGAACC ACCCCGTGAT CCAGTGGTTC
    TGGAAGGCTG TGCTGCTGAT GGACGCCGAG AAGCGCATCC GGTTGCTGCA GTTTGTCACA
    GGGACGTCCC GAGTACCTAT GAATGGATTT GCCGAGCTTT ATGGTTCCAA TGGTCCTCAG
    CTGTTTACAA TAGAGCAATG GGGAAGTCCT GAAAAACTGC CCAGAGCTCA CACGTGCTTT
    AATCGCCTTG ACTTACCTCC GTATGAAACC TTCGAAGATT TACGAGAGAA GCTCCTCATG
    GCTGTGGAAA ACGCCCAAGG CTTCGAGGGG GTGGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa47561
    Clone ID Related Accession (Same CDS sequence) XM_005224351.3 , XM_010819012.2 , XM_015469185.1 , XM_010799401.2 , XM_024984479.1 , XM_015460157.1 , XM_024984477.1 , XM_024984478.1 , XM_015469184.1 , XM_015460156.1 , XM_015460155.1 , XM_015469186.1 , XM_015460156.2 , XM_024984476.1 , XM_010819012.3 , XM_005199752.3 , XM_005224351.4
    Accession Version XM_024984476.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2619bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGCCCAGTGC CCCAGCTGGG
    AGAGCACGTT CATCAACTGT CACTGGTGGT GAGGCGCCAA CGCCATCAGT GGCCTATGTA
    CACACCACGC CGGGCCTGCC CTCGGGCTGG GAAGAGAGGA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCTATCAT GCAGCTCGCA
    GAAGACGGCG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CCGCCCCGCT GGAGGGCGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GATACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAGA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA CAACACAAAG
    ACCACGACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ACATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGCTGGG AAGAAAGGAT CCACTTGGAT
    GGCCGGACGT TTTACATTGA CCACAGCAGC CAGGTGTTAT GTGGCGAGGA CGATGCCAGA
    GACTCAGTGC CCTCGTACGA TAGCAAAATT ACTCAGTGGG AAGACCCAAG ACTGCAGAAC
    CCAGCTATTA CTGGTCCGGC TGTTCCTTAC TCCAGAGAAT TTAAGCAGAA ATATGACTAC
    TTTAGAAAGA AATTAAAGAA ACCTGCGGAT ATTCCAAATA GGTTTGAAAT GAAACTTCAC
    AGAAATAACA TATTTGAAGA GTCCTATCGG AGAATCATGT CTGTGAAAAG ACCAGATGTC
    CTGAAAGCTA GGCTGTGGAT TGAGTTTGAA TCGGAGAAAG GTCTGGACTA CGGGGGCGTG
    GCCAGAGAGT GGTTCTTCCT ACTGTCCAAG GAGATGTTCA ACCCTTACTA CGGCCTCTTC
    GAGTATTCGG CAACGGACAA CTACACACTT CAGATCAATC CCAATTCAGG CCTCTGTAAT
    GAAGATCATT TGTCCTATTT CACTTTCATT GGAAGAGTTG CTGGGCTGGC AGTATTTCAT
    GGGAAACTTC TAGATGGTTT CTTCATTCGA CCGTTTTATA AAATGATGCT GGGAAAGCAG
    ATAACTCTGA ATGACATGGA ATCTGTGGAT AGCGAATATT ACAACTCTTT GAAATGGATC
    TTAGAAAATG ACCCAACTGA ACTGGACCTT ATGTTCTGCA TTGATGAAGA GAACTTTGGG
    CAGACATATC AAGTGGATCT GAAGCCCAAC GGGTCAGAAA TAATGGTAAC GAACGAAAAC
    AAGAGAGAGT ACATCGACCT CGTCATCCAG TGGAGGTTCG TGAACAGGGT CCAGAAGCAG
    ATGAACGCCT TCCTGGAGGG ATTCACGGAA CTGCTCCCTA TTGATTTGAT TAAAATCTTT
    GATGAAAATG AACTGGAGTT GCTGATGTGT GGCCTTGGCG ACGTGGATGT GAACGACTGG
    CGACAACATT CTATTTACAA GAATGGCTAC TGCCCGAACC ACCCCGTGAT CCAGTGGTTC
    TGGAAGGCTG TGCTGCTGAT GGACGCCGAG AAGCGCATCC GGTTGCTGCA GTTTGTCACA
    GGGACGTCCC GAGTACCTAT GAATGGATTT GCCGAGCTTT ATGGTTCCAA TGGTCCTCAG
    CTGTTTACAA TAGAGCAATG GGGAAGTCCT GAAAAACTGC CCAGAGCTCA CACGTGCTTT
    AATCGCCTTG ACTTACCTCC GTATGAAACC TTCGAAGATT TACGAGAGAA GCTCCTCATG
    GCTGTGGAAA ACGCCCAAGG CTTCGAGGGG GTGGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024840244.1
    CDS392..3010
    Translation

    Target ORF information:

    RefSeq Version XM_024984476.1
    Organism Bos taurus(cattle)
    Definition Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024984476.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGCCCAGTGC CCCAGCTGGG
    AGAGCACGTT CATCAACTGT CACTGGTGGT GAGGCGCCAA CGCCATCAGT GGCCTATGTA
    CACACCACGC CGGGCCTGCC CTCGGGCTGG GAAGAGAGGA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCTATCAT GCAGCTCGCA
    GAAGACGGCG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CCGCCCCGCT GGAGGGCGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GATACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAGA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA CAACACAAAG
    ACCACGACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ACATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGCTGGG AAGAAAGGAT CCACTTGGAT
    GGCCGGACGT TTTACATTGA CCACAGCAGC CAGGTGTTAT GTGGCGAGGA CGATGCCAGA
    GACTCAGTGC CCTCGTACGA TAGCAAAATT ACTCAGTGGG AAGACCCAAG ACTGCAGAAC
    CCAGCTATTA CTGGTCCGGC TGTTCCTTAC TCCAGAGAAT TTAAGCAGAA ATATGACTAC
    TTTAGAAAGA AATTAAAGAA ACCTGCGGAT ATTCCAAATA GGTTTGAAAT GAAACTTCAC
    AGAAATAACA TATTTGAAGA GTCCTATCGG AGAATCATGT CTGTGAAAAG ACCAGATGTC
    CTGAAAGCTA GGCTGTGGAT TGAGTTTGAA TCGGAGAAAG GTCTGGACTA CGGGGGCGTG
    GCCAGAGAGT GGTTCTTCCT ACTGTCCAAG GAGATGTTCA ACCCTTACTA CGGCCTCTTC
    GAGTATTCGG CAACGGACAA CTACACACTT CAGATCAATC CCAATTCAGG CCTCTGTAAT
    GAAGATCATT TGTCCTATTT CACTTTCATT GGAAGAGTTG CTGGGCTGGC AGTATTTCAT
    GGGAAACTTC TAGATGGTTT CTTCATTCGA CCGTTTTATA AAATGATGCT GGGAAAGCAG
    ATAACTCTGA ATGACATGGA ATCTGTGGAT AGCGAATATT ACAACTCTTT GAAATGGATC
    TTAGAAAATG ACCCAACTGA ACTGGACCTT ATGTTCTGCA TTGATGAAGA GAACTTTGGG
    CAGACATATC AAGTGGATCT GAAGCCCAAC GGGTCAGAAA TAATGGTAAC GAACGAAAAC
    AAGAGAGAGT ACATCGACCT CGTCATCCAG TGGAGGTTCG TGAACAGGGT CCAGAAGCAG
    ATGAACGCCT TCCTGGAGGG ATTCACGGAA CTGCTCCCTA TTGATTTGAT TAAAATCTTT
    GATGAAAATG AACTGGAGTT GCTGATGTGT GGCCTTGGCG ACGTGGATGT GAACGACTGG
    CGACAACATT CTATTTACAA GAATGGCTAC TGCCCGAACC ACCCCGTGAT CCAGTGGTTC
    TGGAAGGCTG TGCTGCTGAT GGACGCCGAG AAGCGCATCC GGTTGCTGCA GTTTGTCACA
    GGGACGTCCC GAGTACCTAT GAATGGATTT GCCGAGCTTT ATGGTTCCAA TGGTCCTCAG
    CTGTTTACAA TAGAGCAATG GGGAAGTCCT GAAAAACTGC CCAGAGCTCA CACGTGCTTT
    AATCGCCTTG ACTTACCTCC GTATGAAACC TTCGAAGATT TACGAGAGAA GCTCCTCATG
    GCTGTGGAAA ACGCCCAAGG CTTCGAGGGG GTGGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa47561
    Clone ID Related Accession (Same CDS sequence) XM_005224351.3 , XM_010819012.2 , XM_015469185.1 , XM_010799401.2 , XM_024984479.1 , XM_015460157.1 , XM_024984477.1 , XM_024984478.1 , XM_015469184.1 , XM_015460156.1 , XM_015460155.1 , XM_015469186.1 , XM_015460156.2 , XM_024984476.1 , XM_010819012.3 , XM_005199752.3 , XM_005224351.4
    Accession Version XM_010819012.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2619bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010819012.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGCCCAGTGC CCCAGCTGGG
    AGAGCACGTT CATCAACTGT CACTGGTGGT GAGGCGCCAA CGCCATCAGT GGCCTATGTA
    CACACCACGC CGGGCCTGCC CTCGGGCTGG GAAGAGAGGA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCTATCAT GCAGCTCGCA
    GAAGACGGCG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CCGCCCCGCT GGAGGGCGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GATACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAGA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA CAACACAAAG
    ACCACGACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ACATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGCTGGG AAGAAAGGAT CCACTTGGAT
    GGCCGGACGT TTTACATTGA CCACAGCAGC CAGGTGTTAT GTGGCGAGGA CGATGCCAGA
    GACTCAGTGC CCTCGTACGA TAGCAAAATT ACTCAGTGGG AAGACCCAAG ACTGCAGAAC
    CCAGCTATTA CTGGTCCGGC TGTTCCTTAC TCCAGAGAAT TTAAGCAGAA ATATGACTAC
    TTTAGAAAGA AATTAAAGAA ACCTGCGGAT ATTCCAAATA GGTTTGAAAT GAAACTTCAC
    AGAAATAACA TATTTGAAGA GTCCTATCGG AGAATCATGT CTGTGAAAAG ACCAGATGTC
    CTGAAAGCTA GGCTGTGGAT TGAGTTTGAA TCGGAGAAAG GTCTGGACTA CGGGGGCGTG
    GCCAGAGAGT GGTTCTTCCT ACTGTCCAAG GAGATGTTCA ACCCTTACTA CGGCCTCTTC
    GAGTATTCGG CAACGGACAA CTACACACTT CAGATCAATC CCAATTCAGG CCTCTGTAAT
    GAAGATCATT TGTCCTATTT CACTTTCATT GGAAGAGTTG CTGGGCTGGC AGTATTTCAT
    GGGAAACTTC TAGATGGTTT CTTCATTCGA CCGTTTTATA AAATGATGCT GGGAAAGCAG
    ATAACTCTGA ATGACATGGA ATCTGTGGAT AGCGAATATT ACAACTCTTT GAAATGGATC
    TTAGAAAATG ACCCAACTGA ACTGGACCTT ATGTTCTGCA TTGATGAAGA GAACTTTGGG
    CAGACATATC AAGTGGATCT GAAGCCCAAC GGGTCAGAAA TAATGGTAAC GAACGAAAAC
    AAGAGAGAGT ACATCGACCT CGTCATCCAG TGGAGGTTCG TGAACAGGGT CCAGAAGCAG
    ATGAACGCCT TCCTGGAGGG ATTCACGGAA CTGCTCCCTA TTGATTTGAT TAAAATCTTT
    GATGAAAATG AACTGGAGTT GCTGATGTGT GGCCTTGGCG ACGTGGATGT GAACGACTGG
    CGACAACATT CTATTTACAA GAATGGCTAC TGCCCGAACC ACCCCGTGAT CCAGTGGTTC
    TGGAAGGCTG TGCTGCTGAT GGACGCCGAG AAGCGCATCC GGTTGCTGCA GTTTGTCACA
    GGGACGTCCC GAGTACCTAT GAATGGATTT GCCGAGCTTT ATGGTTCCAA TGGTCCTCAG
    CTGTTTACAA TAGAGCAATG GGGAAGTCCT GAAAAACTGC CCAGAGCTCA CACGTGCTTT
    AATCGCCTTG ACTTACCTCC GTATGAAACC TTCGAAGATT TACGAGAGAA GCTCCTCATG
    GCTGTGGAAA ACGCCCAAGG CTTCGAGGGG GTGGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010817314.1
    CDS305..2923
    Translation

    Target ORF information:

    RefSeq Version XM_010819012.3
    Organism Bos taurus(cattle)
    Definition Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X25, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010819012.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGCCCAGTGC CCCAGCTGGG
    AGAGCACGTT CATCAACTGT CACTGGTGGT GAGGCGCCAA CGCCATCAGT GGCCTATGTA
    CACACCACGC CGGGCCTGCC CTCGGGCTGG GAAGAGAGGA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCTATCAT GCAGCTCGCA
    GAAGACGGCG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CCGCCCCGCT GGAGGGCGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GATACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAGA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA CAACACAAAG
    ACCACGACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ACATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGCTGGG AAGAAAGGAT CCACTTGGAT
    GGCCGGACGT TTTACATTGA CCACAGCAGC CAGGTGTTAT GTGGCGAGGA CGATGCCAGA
    GACTCAGTGC CCTCGTACGA TAGCAAAATT ACTCAGTGGG AAGACCCAAG ACTGCAGAAC
    CCAGCTATTA CTGGTCCGGC TGTTCCTTAC TCCAGAGAAT TTAAGCAGAA ATATGACTAC
    TTTAGAAAGA AATTAAAGAA ACCTGCGGAT ATTCCAAATA GGTTTGAAAT GAAACTTCAC
    AGAAATAACA TATTTGAAGA GTCCTATCGG AGAATCATGT CTGTGAAAAG ACCAGATGTC
    CTGAAAGCTA GGCTGTGGAT TGAGTTTGAA TCGGAGAAAG GTCTGGACTA CGGGGGCGTG
    GCCAGAGAGT GGTTCTTCCT ACTGTCCAAG GAGATGTTCA ACCCTTACTA CGGCCTCTTC
    GAGTATTCGG CAACGGACAA CTACACACTT CAGATCAATC CCAATTCAGG CCTCTGTAAT
    GAAGATCATT TGTCCTATTT CACTTTCATT GGAAGAGTTG CTGGGCTGGC AGTATTTCAT
    GGGAAACTTC TAGATGGTTT CTTCATTCGA CCGTTTTATA AAATGATGCT GGGAAAGCAG
    ATAACTCTGA ATGACATGGA ATCTGTGGAT AGCGAATATT ACAACTCTTT GAAATGGATC
    TTAGAAAATG ACCCAACTGA ACTGGACCTT ATGTTCTGCA TTGATGAAGA GAACTTTGGG
    CAGACATATC AAGTGGATCT GAAGCCCAAC GGGTCAGAAA TAATGGTAAC GAACGAAAAC
    AAGAGAGAGT ACATCGACCT CGTCATCCAG TGGAGGTTCG TGAACAGGGT CCAGAAGCAG
    ATGAACGCCT TCCTGGAGGG ATTCACGGAA CTGCTCCCTA TTGATTTGAT TAAAATCTTT
    GATGAAAATG AACTGGAGTT GCTGATGTGT GGCCTTGGCG ACGTGGATGT GAACGACTGG
    CGACAACATT CTATTTACAA GAATGGCTAC TGCCCGAACC ACCCCGTGAT CCAGTGGTTC
    TGGAAGGCTG TGCTGCTGAT GGACGCCGAG AAGCGCATCC GGTTGCTGCA GTTTGTCACA
    GGGACGTCCC GAGTACCTAT GAATGGATTT GCCGAGCTTT ATGGTTCCAA TGGTCCTCAG
    CTGTTTACAA TAGAGCAATG GGGAAGTCCT GAAAAACTGC CCAGAGCTCA CACGTGCTTT
    AATCGCCTTG ACTTACCTCC GTATGAAACC TTCGAAGATT TACGAGAGAA GCTCCTCATG
    GCTGTGGAAA ACGCCCAAGG CTTCGAGGGG GTGGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa47561
    Clone ID Related Accession (Same CDS sequence) XM_005224351.3 , XM_010819012.2 , XM_015469185.1 , XM_010799401.2 , XM_024984479.1 , XM_015460157.1 , XM_024984477.1 , XM_024984478.1 , XM_015469184.1 , XM_015460156.1 , XM_015460155.1 , XM_015469186.1 , XM_015460156.2 , XM_024984476.1 , XM_010819012.3 , XM_005199752.3 , XM_005224351.4
    Accession Version XM_005199752.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2619bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646636.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005199752.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGCCCAGTGC CCCAGCTGGG
    AGAGCACGTT CATCAACTGT CACTGGTGGT GAGGCGCCAA CGCCATCAGT GGCCTATGTA
    CACACCACGC CGGGCCTGCC CTCGGGCTGG GAAGAGAGGA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCTATCAT GCAGCTCGCA
    GAAGACGGCG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CCGCCCCGCT GGAGGGCGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GATACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAGA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA CAACACAAAG
    ACCACGACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ACATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGCTGGG AAGAAAGGAT CCACTTGGAT
    GGCCGGACGT TTTACATTGA CCACAGCAGC CAGGTGTTAT GTGGCGAGGA CGATGCCAGA
    GACTCAGTGC CCTCGTACGA TAGCAAAATT ACTCAGTGGG AAGACCCAAG ACTGCAGAAC
    CCAGCTATTA CTGGTCCGGC TGTTCCTTAC TCCAGAGAAT TTAAGCAGAA ATATGACTAC
    TTTAGAAAGA AATTAAAGAA ACCTGCGGAT ATTCCAAATA GGTTTGAAAT GAAACTTCAC
    AGAAATAACA TATTTGAAGA GTCCTATCGG AGAATCATGT CTGTGAAAAG ACCAGATGTC
    CTGAAAGCTA GGCTGTGGAT TGAGTTTGAA TCGGAGAAAG GTCTGGACTA CGGGGGCGTG
    GCCAGAGAGT GGTTCTTCCT ACTGTCCAAG GAGATGTTCA ACCCTTACTA CGGCCTCTTC
    GAGTATTCGG CAACGGACAA CTACACACTT CAGATCAATC CCAATTCAGG CCTCTGTAAT
    GAAGATCATT TGTCCTATTT CACTTTCATT GGAAGAGTTG CTGGGCTGGC AGTATTTCAT
    GGGAAACTTC TAGATGGTTT CTTCATTCGA CCGTTTTATA AAATGATGCT GGGAAAGCAG
    ATAACTCTGA ATGACATGGA ATCTGTGGAT AGCGAATATT ACAACTCTTT GAAATGGATC
    TTAGAAAATG ACCCAACTGA ACTGGACCTT ATGTTCTGCA TTGATGAAGA GAACTTTGGG
    CAGACATATC AAGTGGATCT GAAGCCCAAC GGGTCAGAAA TAATGGTAAC GAACGAAAAC
    AAGAGAGAGT ACATCGACCT CGTCATCCAG TGGAGGTTCG TGAACAGGGT CCAGAAGCAG
    ATGAACGCCT TCCTGGAGGG ATTCACGGAA CTGCTCCCTA TTGATTTGAT TAAAATCTTT
    GATGAAAATG AACTGGAGTT GCTGATGTGT GGCCTTGGCG ACGTGGATGT GAACGACTGG
    CGACAACATT CTATTTACAA GAATGGCTAC TGCCCGAACC ACCCCGTGAT CCAGTGGTTC
    TGGAAGGCTG TGCTGCTGAT GGACGCCGAG AAGCGCATCC GGTTGCTGCA GTTTGTCACA
    GGGACGTCCC GAGTACCTAT GAATGGATTT GCCGAGCTTT ATGGTTCCAA TGGTCCTCAG
    CTGTTTACAA TAGAGCAATG GGGAAGTCCT GAAAAACTGC CCAGAGCTCA CACGTGCTTT
    AATCGCCTTG ACTTACCTCC GTATGAAACC TTCGAAGATT TACGAGAGAA GCTCCTCATG
    GCTGTGGAAA ACGCCCAAGG CTTCGAGGGG GTGGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005199809.3
    CDS559..3177
    Translation

    Target ORF information:

    RefSeq Version XM_005199752.3
    Organism Bos taurus(cattle)
    Definition Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005199752.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGCCCAGTGC CCCAGCTGGG
    AGAGCACGTT CATCAACTGT CACTGGTGGT GAGGCGCCAA CGCCATCAGT GGCCTATGTA
    CACACCACGC CGGGCCTGCC CTCGGGCTGG GAAGAGAGGA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCTATCAT GCAGCTCGCA
    GAAGACGGCG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CCGCCCCGCT GGAGGGCGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GATACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAGA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA CAACACAAAG
    ACCACGACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ACATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGCTGGG AAGAAAGGAT CCACTTGGAT
    GGCCGGACGT TTTACATTGA CCACAGCAGC CAGGTGTTAT GTGGCGAGGA CGATGCCAGA
    GACTCAGTGC CCTCGTACGA TAGCAAAATT ACTCAGTGGG AAGACCCAAG ACTGCAGAAC
    CCAGCTATTA CTGGTCCGGC TGTTCCTTAC TCCAGAGAAT TTAAGCAGAA ATATGACTAC
    TTTAGAAAGA AATTAAAGAA ACCTGCGGAT ATTCCAAATA GGTTTGAAAT GAAACTTCAC
    AGAAATAACA TATTTGAAGA GTCCTATCGG AGAATCATGT CTGTGAAAAG ACCAGATGTC
    CTGAAAGCTA GGCTGTGGAT TGAGTTTGAA TCGGAGAAAG GTCTGGACTA CGGGGGCGTG
    GCCAGAGAGT GGTTCTTCCT ACTGTCCAAG GAGATGTTCA ACCCTTACTA CGGCCTCTTC
    GAGTATTCGG CAACGGACAA CTACACACTT CAGATCAATC CCAATTCAGG CCTCTGTAAT
    GAAGATCATT TGTCCTATTT CACTTTCATT GGAAGAGTTG CTGGGCTGGC AGTATTTCAT
    GGGAAACTTC TAGATGGTTT CTTCATTCGA CCGTTTTATA AAATGATGCT GGGAAAGCAG
    ATAACTCTGA ATGACATGGA ATCTGTGGAT AGCGAATATT ACAACTCTTT GAAATGGATC
    TTAGAAAATG ACCCAACTGA ACTGGACCTT ATGTTCTGCA TTGATGAAGA GAACTTTGGG
    CAGACATATC AAGTGGATCT GAAGCCCAAC GGGTCAGAAA TAATGGTAAC GAACGAAAAC
    AAGAGAGAGT ACATCGACCT CGTCATCCAG TGGAGGTTCG TGAACAGGGT CCAGAAGCAG
    ATGAACGCCT TCCTGGAGGG ATTCACGGAA CTGCTCCCTA TTGATTTGAT TAAAATCTTT
    GATGAAAATG AACTGGAGTT GCTGATGTGT GGCCTTGGCG ACGTGGATGT GAACGACTGG
    CGACAACATT CTATTTACAA GAATGGCTAC TGCCCGAACC ACCCCGTGAT CCAGTGGTTC
    TGGAAGGCTG TGCTGCTGAT GGACGCCGAG AAGCGCATCC GGTTGCTGCA GTTTGTCACA
    GGGACGTCCC GAGTACCTAT GAATGGATTT GCCGAGCTTT ATGGTTCCAA TGGTCCTCAG
    CTGTTTACAA TAGAGCAATG GGGAAGTCCT GAAAAACTGC CCAGAGCTCA CACGTGCTTT
    AATCGCCTTG ACTTACCTCC GTATGAAACC TTCGAAGATT TACGAGAGAA GCTCCTCATG
    GCTGTGGAAA ACGCCCAAGG CTTCGAGGGG GTGGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa47561
    Clone ID Related Accession (Same CDS sequence) XM_005224351.3 , XM_010819012.2 , XM_015469185.1 , XM_010799401.2 , XM_024984479.1 , XM_015460157.1 , XM_024984477.1 , XM_024984478.1 , XM_015469184.1 , XM_015460156.1 , XM_015460155.1 , XM_015469186.1 , XM_015460156.2 , XM_024984476.1 , XM_010819012.3 , XM_005199752.3 , XM_005224351.4
    Accession Version XM_005224351.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2619bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005224351.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGCCCAGTGC CCCAGCTGGG
    AGAGCACGTT CATCAACTGT CACTGGTGGT GAGGCGCCAA CGCCATCAGT GGCCTATGTA
    CACACCACGC CGGGCCTGCC CTCGGGCTGG GAAGAGAGGA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCTATCAT GCAGCTCGCA
    GAAGACGGCG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CCGCCCCGCT GGAGGGCGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GATACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAGA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA CAACACAAAG
    ACCACGACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ACATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGCTGGG AAGAAAGGAT CCACTTGGAT
    GGCCGGACGT TTTACATTGA CCACAGCAGC CAGGTGTTAT GTGGCGAGGA CGATGCCAGA
    GACTCAGTGC CCTCGTACGA TAGCAAAATT ACTCAGTGGG AAGACCCAAG ACTGCAGAAC
    CCAGCTATTA CTGGTCCGGC TGTTCCTTAC TCCAGAGAAT TTAAGCAGAA ATATGACTAC
    TTTAGAAAGA AATTAAAGAA ACCTGCGGAT ATTCCAAATA GGTTTGAAAT GAAACTTCAC
    AGAAATAACA TATTTGAAGA GTCCTATCGG AGAATCATGT CTGTGAAAAG ACCAGATGTC
    CTGAAAGCTA GGCTGTGGAT TGAGTTTGAA TCGGAGAAAG GTCTGGACTA CGGGGGCGTG
    GCCAGAGAGT GGTTCTTCCT ACTGTCCAAG GAGATGTTCA ACCCTTACTA CGGCCTCTTC
    GAGTATTCGG CAACGGACAA CTACACACTT CAGATCAATC CCAATTCAGG CCTCTGTAAT
    GAAGATCATT TGTCCTATTT CACTTTCATT GGAAGAGTTG CTGGGCTGGC AGTATTTCAT
    GGGAAACTTC TAGATGGTTT CTTCATTCGA CCGTTTTATA AAATGATGCT GGGAAAGCAG
    ATAACTCTGA ATGACATGGA ATCTGTGGAT AGCGAATATT ACAACTCTTT GAAATGGATC
    TTAGAAAATG ACCCAACTGA ACTGGACCTT ATGTTCTGCA TTGATGAAGA GAACTTTGGG
    CAGACATATC AAGTGGATCT GAAGCCCAAC GGGTCAGAAA TAATGGTAAC GAACGAAAAC
    AAGAGAGAGT ACATCGACCT CGTCATCCAG TGGAGGTTCG TGAACAGGGT CCAGAAGCAG
    ATGAACGCCT TCCTGGAGGG ATTCACGGAA CTGCTCCCTA TTGATTTGAT TAAAATCTTT
    GATGAAAATG AACTGGAGTT GCTGATGTGT GGCCTTGGCG ACGTGGATGT GAACGACTGG
    CGACAACATT CTATTTACAA GAATGGCTAC TGCCCGAACC ACCCCGTGAT CCAGTGGTTC
    TGGAAGGCTG TGCTGCTGAT GGACGCCGAG AAGCGCATCC GGTTGCTGCA GTTTGTCACA
    GGGACGTCCC GAGTACCTAT GAATGGATTT GCCGAGCTTT ATGGTTCCAA TGGTCCTCAG
    CTGTTTACAA TAGAGCAATG GGGAAGTCCT GAAAAACTGC CCAGAGCTCA CACGTGCTTT
    AATCGCCTTG ACTTACCTCC GTATGAAACC TTCGAAGATT TACGAGAGAA GCTCCTCATG
    GCTGTGGAAA ACGCCCAAGG CTTCGAGGGG GTGGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005224408.3
    CDS559..3177
    Translation

    Target ORF information:

    RefSeq Version XM_005224351.4
    Organism Bos taurus(cattle)
    Definition Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005224351.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGCCCAGTGC CCCAGCTGGG
    AGAGCACGTT CATCAACTGT CACTGGTGGT GAGGCGCCAA CGCCATCAGT GGCCTATGTA
    CACACCACGC CGGGCCTGCC CTCGGGCTGG GAAGAGAGGA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCTATCAT GCAGCTCGCA
    GAAGACGGCG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CCGCCCCGCT GGAGGGCGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GATACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAGA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA CAACACAAAG
    ACCACGACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ACATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGCTGGG AAGAAAGGAT CCACTTGGAT
    GGCCGGACGT TTTACATTGA CCACAGCAGC CAGGTGTTAT GTGGCGAGGA CGATGCCAGA
    GACTCAGTGC CCTCGTACGA TAGCAAAATT ACTCAGTGGG AAGACCCAAG ACTGCAGAAC
    CCAGCTATTA CTGGTCCGGC TGTTCCTTAC TCCAGAGAAT TTAAGCAGAA ATATGACTAC
    TTTAGAAAGA AATTAAAGAA ACCTGCGGAT ATTCCAAATA GGTTTGAAAT GAAACTTCAC
    AGAAATAACA TATTTGAAGA GTCCTATCGG AGAATCATGT CTGTGAAAAG ACCAGATGTC
    CTGAAAGCTA GGCTGTGGAT TGAGTTTGAA TCGGAGAAAG GTCTGGACTA CGGGGGCGTG
    GCCAGAGAGT GGTTCTTCCT ACTGTCCAAG GAGATGTTCA ACCCTTACTA CGGCCTCTTC
    GAGTATTCGG CAACGGACAA CTACACACTT CAGATCAATC CCAATTCAGG CCTCTGTAAT
    GAAGATCATT TGTCCTATTT CACTTTCATT GGAAGAGTTG CTGGGCTGGC AGTATTTCAT
    GGGAAACTTC TAGATGGTTT CTTCATTCGA CCGTTTTATA AAATGATGCT GGGAAAGCAG
    ATAACTCTGA ATGACATGGA ATCTGTGGAT AGCGAATATT ACAACTCTTT GAAATGGATC
    TTAGAAAATG ACCCAACTGA ACTGGACCTT ATGTTCTGCA TTGATGAAGA GAACTTTGGG
    CAGACATATC AAGTGGATCT GAAGCCCAAC GGGTCAGAAA TAATGGTAAC GAACGAAAAC
    AAGAGAGAGT ACATCGACCT CGTCATCCAG TGGAGGTTCG TGAACAGGGT CCAGAAGCAG
    ATGAACGCCT TCCTGGAGGG ATTCACGGAA CTGCTCCCTA TTGATTTGAT TAAAATCTTT
    GATGAAAATG AACTGGAGTT GCTGATGTGT GGCCTTGGCG ACGTGGATGT GAACGACTGG
    CGACAACATT CTATTTACAA GAATGGCTAC TGCCCGAACC ACCCCGTGAT CCAGTGGTTC
    TGGAAGGCTG TGCTGCTGAT GGACGCCGAG AAGCGCATCC GGTTGCTGCA GTTTGTCACA
    GGGACGTCCC GAGTACCTAT GAATGGATTT GCCGAGCTTT ATGGTTCCAA TGGTCCTCAG
    CTGTTTACAA TAGAGCAATG GGGAAGTCCT GAAAAACTGC CCAGAGCTCA CACGTGCTTT
    AATCGCCTTG ACTTACCTCC GTATGAAACC TTCGAAGATT TACGAGAGAA GCTCCTCATG
    GCTGTGGAAA ACGCCCAAGG CTTCGAGGGG GTGGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa230043
    Clone ID Related Accession (Same CDS sequence) XM_024984471.1
    Accession Version XM_024984471.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2982bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCGACCG GGCTCGGGGA GCCGGTCTAC GGACTTTCGG AAGAAGAGGG AGAGTCGCGT 
    ATTCTCAGAG TGAAGGTTGT TTCTGGAATT GATCTCGCCA AGAAGGACAT CTTCGGAGCC
    AGTGATCCAT ACGTGAAACT TTCACTGTAC GTAGCGGATG AGAATCGGGA ACTTGCTTTG
    GTACAAACAA AAACAATTAA AAAGACGCTG AACCCAAAGT GGAATGAAGA ATTTTATTTT
    AGAGTAAATC CATCTAATCA CAGACTCCTG TTTGAAGTAT TTGATGAAAA CAGACTGACA
    CGGGACGACT TCCTGGGACA GGTGGACATA CCCCTTAGTC ATCTCCCGAC AGAAGACCCA
    ACCATGGAGC GACCCTACAC ATTTAAGGAC TTTCTCCTCC GGCCAAGAAG TCATAAATCT
    CGGGTTAAGG GATTTTTGCG ATTGAAAATG GCCTATATGC CCAAAAACGG AGGCCAAGAT
    GAAGAGAACA GTGAGCAGCG GGATGAGATG GAGCCCGGGT GGGAAGTCGT TGACTCCAAT
    GACTCGGCTT CTCAGCACCA GGAGGAGCTG CCCCCGCCTC CGCTGCCCCC CGGGTGGGAA
    GAGAAGGTGG ACAACCTGGG CCGCACGTAC TACGTCAACC ACAACAACCG GACCACCCAG
    TGGCACCGGC CCAGCCTCAT GGACGTGTCG TCCGAGTCCG ACAGCAACAT CCGGCAGATC
    AACCAGGAGG CCGCGCATCG CCGCTTCCGC TCGCGCAGAC ATATCAGCGA GGACCTGGAG
    CCGGAGCCCA GCGAGGGCGG GGAGGGCCCC GAGCCTTGGG AGACCATTTC AGAGGAAGTG
    AACATGAGCG GAGACTCGCT CAGCCTGGCG CTGCCCCCGC CACCGGCCTC GCCCGTGTCC
    CGGACGAGCC CCCAGGAGCT GTCCGAGGAG CTGAGCAGAA GGCTGCAGGT CACTCCCGAC
    TCCAACGGCG AGCAGTTTGG GTCTTTGATT CAGAGAGAGC CGTCCTCCAG GCTGAGGTCC
    TGCAGTGTCA CCGACGCAGT CGCGGAGCAG GCTCACCTCC CGCCGCCCAG TGCCCCAGCT
    GGGAGAGCAC GTTCATCAAC TGTCACTGGT GGTGAGGCGC CAACGCCATC AGTGGCCTAT
    GTACACACCA CGCCGGGCCT GCCCTCGGGC TGGGAAGAGA GGAAAGATGC TAAGGGACGC
    ACATACTATG TCAATCATAA CAATCGAACC ACAACTTGGA CTCGGCCTAT CATGCAGCTC
    GCAGAAGACG GCGCGTCCGG ATCCGCCACA AACAGTAACA ACCACCTAAT CGAGCCTCAG
    ATCCGCCGGC CTCGTAGCCT CAGCTCGCCA ACAGTAACTT TATCCGCCCC GCTGGAGGGC
    GCCAAGGACT CACCCGTACG TCGGGCTGTG AAAGATACCC TTTCCAACCC ACAGTCCCCA
    CAGCCATCAC CTTACAACTC CCCCAAACCA CAACACAAAG TCACACAGAG CTTCCTGCCG
    CCCGGCTGGG AGATGAGGAT AGCGCCCAAC GGCCGGCCCT TCTTCATTGA CCACAACACA
    AAGACCACGA CGTGGGAAGA TCCACGCCTG AAATTTCCAG TACACATGCG GTCAAAGGCA
    TCCTTAAACC CCAATGACCT TGGCCCTCTT CCTCCTGGCT GGGAAGAAAG GATCCACTTG
    GATGGCCGGA CGTTTTACAT TGACCACAGC AGCCAGGTGT TATGTGGCGA GGACGATGCC
    AGAGACTCAG TGCCCTCGTA CGATAGCAAA ATTACTCAGT GGGAAGACCC AAGACTGCAG
    AACCCAGCTA TTACTGGTCC GGCTGTTCCT TACTCCAGAG AATTTAAGCA GAAATATGAC
    TACTTTAGAA AGAAATTAAA GAAACCTGCG GATATTCCAA ATAGGTTTGA AATGAAACTT
    CACAGAAATA ACATATTTGA AGAGTCCTAT CGGAGAATCA TGTCTGTGAA AAGACCAGAT
    GTCCTGAAAG CTAGGCTGTG GATTGAGTTT GAATCGGAGA AAGGTCTGGA CTACGGGGGC
    GTGGCCAGAG AGTGGTTCTT CCTACTGTCC AAGGAGATGT TCAACCCTTA CTACGGCCTC
    TTCGAGTATT CGGCAACGGA CAACTACACA CTTCAGATCA ATCCCAATTC AGGCCTCTGT
    AATGAAGATC ATTTGTCCTA TTTCACTTTC ATTGGAAGAG TTGCTGGGCT GGCAGTATTT
    CATGGGAAAC TTCTAGATGG TTTCTTCATT CGACCGTTTT ATAAAATGAT GCTGGGAAAG
    CAGATAACTC TGAATGACAT GGAATCTGTG GATAGCGAAT ATTACAACTC TTTGAAATGG
    ATCTTAGAAA ATGACCCAAC TGAACTGGAC CTTATGTTCT GCATTGATGA AGAGAACTTT
    GGGCAGACAT ATCAAGTGGA TCTGAAGCCC AACGGGTCAG AAATAATGGT AACGAACGAA
    AACAAGAGAG AGTACATCGA CCTCGTCATC CAGTGGAGGT TCGTGAACAG GGTCCAGAAG
    CAGATGAACG CCTTCCTGGA GGGATTCACG GAACTGCTCC CTATTGATTT GATTAAAATC
    TTTGATGAAA ATGAACTGGA GTTGCTGATG TGTGGCCTTG GCGACGTGGA TGTGAACGAC
    TGGCGACAAC ATTCTATTTA CAAGAATGGC TACTGCCCGA ACCACCCCGT GATCCAGTGG
    TTCTGGAAGG CTGTGCTGCT GATGGACGCC GAGAAGCGCA TCCGGTTGCT GCAGTTTGTC
    ACAGGGACGT CCCGAGTACC TATGAATGGA TTTGCCGAGC TTTATGGTTC CAATGGTCCT
    CAGCTGTTTA CAATAGAGCA ATGGGGAAGT CCTGAAAAAC TGCCCAGAGC TCACACGTGC
    TTTAATCGCC TTGACTTACC TCCGTATGAA ACCTTCGAAG ATTTACGAGA GAAGCTCCTC
    ATGGCTGTGG AAAACGCCCA AGGCTTCGAG GGGGTGGATT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024840239.1
    CDS169..3150
    Translation

    Target ORF information:

    RefSeq Version XM_024984471.1
    Organism Bos taurus(cattle)
    Definition Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024984471.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCGACCG GGCTCGGGGA GCCGGTCTAC GGACTTTCGG AAGAAGAGGG AGAGTCGCGT 
    ATTCTCAGAG TGAAGGTTGT TTCTGGAATT GATCTCGCCA AGAAGGACAT CTTCGGAGCC
    AGTGATCCAT ACGTGAAACT TTCACTGTAC GTAGCGGATG AGAATCGGGA ACTTGCTTTG
    GTACAAACAA AAACAATTAA AAAGACGCTG AACCCAAAGT GGAATGAAGA ATTTTATTTT
    AGAGTAAATC CATCTAATCA CAGACTCCTG TTTGAAGTAT TTGATGAAAA CAGACTGACA
    CGGGACGACT TCCTGGGACA GGTGGACATA CCCCTTAGTC ATCTCCCGAC AGAAGACCCA
    ACCATGGAGC GACCCTACAC ATTTAAGGAC TTTCTCCTCC GGCCAAGAAG TCATAAATCT
    CGGGTTAAGG GATTTTTGCG ATTGAAAATG GCCTATATGC CCAAAAACGG AGGCCAAGAT
    GAAGAGAACA GTGAGCAGCG GGATGAGATG GAGCCCGGGT GGGAAGTCGT TGACTCCAAT
    GACTCGGCTT CTCAGCACCA GGAGGAGCTG CCCCCGCCTC CGCTGCCCCC CGGGTGGGAA
    GAGAAGGTGG ACAACCTGGG CCGCACGTAC TACGTCAACC ACAACAACCG GACCACCCAG
    TGGCACCGGC CCAGCCTCAT GGACGTGTCG TCCGAGTCCG ACAGCAACAT CCGGCAGATC
    AACCAGGAGG CCGCGCATCG CCGCTTCCGC TCGCGCAGAC ATATCAGCGA GGACCTGGAG
    CCGGAGCCCA GCGAGGGCGG GGAGGGCCCC GAGCCTTGGG AGACCATTTC AGAGGAAGTG
    AACATGAGCG GAGACTCGCT CAGCCTGGCG CTGCCCCCGC CACCGGCCTC GCCCGTGTCC
    CGGACGAGCC CCCAGGAGCT GTCCGAGGAG CTGAGCAGAA GGCTGCAGGT CACTCCCGAC
    TCCAACGGCG AGCAGTTTGG GTCTTTGATT CAGAGAGAGC CGTCCTCCAG GCTGAGGTCC
    TGCAGTGTCA CCGACGCAGT CGCGGAGCAG GCTCACCTCC CGCCGCCCAG TGCCCCAGCT
    GGGAGAGCAC GTTCATCAAC TGTCACTGGT GGTGAGGCGC CAACGCCATC AGTGGCCTAT
    GTACACACCA CGCCGGGCCT GCCCTCGGGC TGGGAAGAGA GGAAAGATGC TAAGGGACGC
    ACATACTATG TCAATCATAA CAATCGAACC ACAACTTGGA CTCGGCCTAT CATGCAGCTC
    GCAGAAGACG GCGCGTCCGG ATCCGCCACA AACAGTAACA ACCACCTAAT CGAGCCTCAG
    ATCCGCCGGC CTCGTAGCCT CAGCTCGCCA ACAGTAACTT TATCCGCCCC GCTGGAGGGC
    GCCAAGGACT CACCCGTACG TCGGGCTGTG AAAGATACCC TTTCCAACCC ACAGTCCCCA
    CAGCCATCAC CTTACAACTC CCCCAAACCA CAACACAAAG TCACACAGAG CTTCCTGCCG
    CCCGGCTGGG AGATGAGGAT AGCGCCCAAC GGCCGGCCCT TCTTCATTGA CCACAACACA
    AAGACCACGA CGTGGGAAGA TCCACGCCTG AAATTTCCAG TACACATGCG GTCAAAGGCA
    TCCTTAAACC CCAATGACCT TGGCCCTCTT CCTCCTGGCT GGGAAGAAAG GATCCACTTG
    GATGGCCGGA CGTTTTACAT TGACCACAGC AGCCAGGTGT TATGTGGCGA GGACGATGCC
    AGAGACTCAG TGCCCTCGTA CGATAGCAAA ATTACTCAGT GGGAAGACCC AAGACTGCAG
    AACCCAGCTA TTACTGGTCC GGCTGTTCCT TACTCCAGAG AATTTAAGCA GAAATATGAC
    TACTTTAGAA AGAAATTAAA GAAACCTGCG GATATTCCAA ATAGGTTTGA AATGAAACTT
    CACAGAAATA ACATATTTGA AGAGTCCTAT CGGAGAATCA TGTCTGTGAA AAGACCAGAT
    GTCCTGAAAG CTAGGCTGTG GATTGAGTTT GAATCGGAGA AAGGTCTGGA CTACGGGGGC
    GTGGCCAGAG AGTGGTTCTT CCTACTGTCC AAGGAGATGT TCAACCCTTA CTACGGCCTC
    TTCGAGTATT CGGCAACGGA CAACTACACA CTTCAGATCA ATCCCAATTC AGGCCTCTGT
    AATGAAGATC ATTTGTCCTA TTTCACTTTC ATTGGAAGAG TTGCTGGGCT GGCAGTATTT
    CATGGGAAAC TTCTAGATGG TTTCTTCATT CGACCGTTTT ATAAAATGAT GCTGGGAAAG
    CAGATAACTC TGAATGACAT GGAATCTGTG GATAGCGAAT ATTACAACTC TTTGAAATGG
    ATCTTAGAAA ATGACCCAAC TGAACTGGAC CTTATGTTCT GCATTGATGA AGAGAACTTT
    GGGCAGACAT ATCAAGTGGA TCTGAAGCCC AACGGGTCAG AAATAATGGT AACGAACGAA
    AACAAGAGAG AGTACATCGA CCTCGTCATC CAGTGGAGGT TCGTGAACAG GGTCCAGAAG
    CAGATGAACG CCTTCCTGGA GGGATTCACG GAACTGCTCC CTATTGATTT GATTAAAATC
    TTTGATGAAA ATGAACTGGA GTTGCTGATG TGTGGCCTTG GCGACGTGGA TGTGAACGAC
    TGGCGACAAC ATTCTATTTA CAAGAATGGC TACTGCCCGA ACCACCCCGT GATCCAGTGG
    TTCTGGAAGG CTGTGCTGCT GATGGACGCC GAGAAGCGCA TCCGGTTGCT GCAGTTTGTC
    ACAGGGACGT CCCGAGTACC TATGAATGGA TTTGCCGAGC TTTATGGTTC CAATGGTCCT
    CAGCTGTTTA CAATAGAGCA ATGGGGAAGT CCTGAAAAAC TGCCCAGAGC TCACACGTGC
    TTTAATCGCC TTGACTTACC TCCGTATGAA ACCTTCGAAG ATTTACGAGA GAAGCTCCTC
    ATGGCTGTGG AAAACGCCCA AGGCTTCGAG GGGGTGGATT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa230044
    Clone ID Related Accession (Same CDS sequence) XM_024984472.1
    Accession Version XM_024984472.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2928bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGCGACCG GGCTCGGGGA GCCGGTCTAC GGACTTTCGG AAGAAGAGGG AGAGTCGCGT 
    ATTCTCAGAG TGAAGGTTGT TTCTGGAATT GATCTCGCCA AGAAGGACAT CTTCGGAGCC
    AGTGATCCAT ACGTGAAACT TTCACTGTAC GTAGCGGATG AGAATCGGGA ACTTGCTTTG
    GTACAAACAA AAACAATTAA AAAGACGCTG AACCCAAAGT GGAATGAAGA ATTTTATTTT
    AGAGTAAATC CATCTAATCA CAGACTCCTG TTTGAAGTAT TTGATGAAAA CAGACTGACA
    CGGGACGACT TCCTGGGACA GGTGGACATA CCCCTTAGTC ATCTCCCGAC AGAAGACCCA
    ACCATGGAGC GACCCTACAC ATTTAAGGAC TTTCTCCTCC GGCCAAGAAG TCATAAATCT
    CGGGTTAAGG GATTTTTGCG ATTGAAAATG GCCTATATGC CCAAAAACGG AGGCCAAGAT
    GAAGAGAACA GTGAGCAGCG GGATGAGATG GAGCCCGGGT GGGAAGTCGT TGACTCCAAT
    GACTCGGCTT CTCAGCACCA GGAGGAGCTG CCCCCGCCTC CGCTGCCCCC CGGGTGGGAA
    GAGAAGGTGG ACAACCTGGG CCGCACGTAC TACGTCAACC ACAACAACCG GACCACCCAG
    TGGCACCGGC CCAGCCTCAT GGACGTGTCG TCCGAGTCCG ACAGCAACAT CCGGCAGATC
    AACCAGGAGG CCGCGCATCG CCGCTTCCGC TCGCGCAGAC ATATCAGCGA GGACCTGGAG
    CCGGAGCCCA GCGAGGGCGG GGAGGGCCCC GAGCCTTGGG AGACCATTTC AGAGGAAGTG
    AACATGAGCG GAGACTCGCT CAGCCTGGCG CTGCCCCCGC CACCGGCCTC GCCCGTGTCC
    CGGACGAGCC CCCAGGAGCT GTCCGAGGAG CTGAGCAGAA GGCTGCAGGT CACTCCCGAC
    TCCAACGGCG AGCAGTTTGG GTCTTTGATT CAGAGAGAGC CGTCCTCCAG GCTGAGGTCC
    TGCAGTGTCA CCGACGCAGT CGCGGAGCAG GCTCACCTCC CGCCGCCCAG TGCCCCAGCT
    GGGAGAGCAC GTTCATCAAC TGTCACTGGT GGTGAGGCGC CAACGCCATC AGTGGCCTAT
    GTACACACCA CGCCGGGCCT GCCCTCGGGC TGGGAAGAGA GGAAAGATGC TAAGGGACGC
    ACATACTATG TCAATCATAA CAATCGAACC ACAACTTGGA CTCGGCCTAT CATGCAGCTC
    GCAGAAGACG GCGCGTCCGG ATCCGCCACA AACAGTAACA ACCACCTAAT CGAGCCTCAG
    ATCCGCCGGC CTCGTAGCCT CAGCTCGCCA ACAGTAACTT TATCCGCCCC GCTGGAGGGC
    GCCAAGGACT CACCCGTACG TCGGGCTGTG AAAGATACCC TTTCCAACCC ACAGTCCCCA
    CAGCCATCAC CTTACAACTC CCCCAAACCA CAACACAAAG TCACACAGAG CTTCCTGCCG
    CCCGGCTGGG AGATGAGGAT AGCGCCCAAC GGCCGGCCCT TCTTCATTGA CCACAACACA
    AAGACCACGA CGTGGGAAGA TCCACGCCTG AAATTTCCAG TACACATGCG GTCAAAGGCA
    TCCTTAAACC CCAATGACCT TGGCCCTCTT CCTCCTGGCT GGGAAGAAAG GATCCACTTG
    GATGGCCGGA CGTTTTACAT TGACCACAAT AGCAAAATTA CTCAGTGGGA AGACCCAAGA
    CTGCAGAACC CAGCTATTAC TGGTCCGGCT GTTCCTTACT CCAGAGAATT TAAGCAGAAA
    TATGACTACT TTAGAAAGAA ATTAAAGAAA CCTGCGGATA TTCCAAATAG GTTTGAAATG
    AAACTTCACA GAAATAACAT ATTTGAAGAG TCCTATCGGA GAATCATGTC TGTGAAAAGA
    CCAGATGTCC TGAAAGCTAG GCTGTGGATT GAGTTTGAAT CGGAGAAAGG TCTGGACTAC
    GGGGGCGTGG CCAGAGAGTG GTTCTTCCTA CTGTCCAAGG AGATGTTCAA CCCTTACTAC
    GGCCTCTTCG AGTATTCGGC AACGGACAAC TACACACTTC AGATCAATCC CAATTCAGGC
    CTCTGTAATG AAGATCATTT GTCCTATTTC ACTTTCATTG GAAGAGTTGC TGGGCTGGCA
    GTATTTCATG GGAAACTTCT AGATGGTTTC TTCATTCGAC CGTTTTATAA AATGATGCTG
    GGAAAGCAGA TAACTCTGAA TGACATGGAA TCTGTGGATA GCGAATATTA CAACTCTTTG
    AAATGGATCT TAGAAAATGA CCCAACTGAA CTGGACCTTA TGTTCTGCAT TGATGAAGAG
    AACTTTGGGC AGACATATCA AGTGGATCTG AAGCCCAACG GGTCAGAAAT AATGGTAACG
    AACGAAAACA AGAGAGAGTA CATCGACCTC GTCATCCAGT GGAGGTTCGT GAACAGGGTC
    CAGAAGCAGA TGAACGCCTT CCTGGAGGGA TTCACGGAAC TGCTCCCTAT TGATTTGATT
    AAAATCTTTG ATGAAAATGA ACTGGAGTTG CTGATGTGTG GCCTTGGCGA CGTGGATGTG
    AACGACTGGC GACAACATTC TATTTACAAG AATGGCTACT GCCCGAACCA CCCCGTGATC
    CAGTGGTTCT GGAAGGCTGT GCTGCTGATG GACGCCGAGA AGCGCATCCG GTTGCTGCAG
    TTTGTCACAG GGACGTCCCG AGTACCTATG AATGGATTTG CCGAGCTTTA TGGTTCCAAT
    GGTCCTCAGC TGTTTACAAT AGAGCAATGG GGAAGTCCTG AAAAACTGCC CAGAGCTCAC
    ACGTGCTTTA ATCGCCTTGA CTTACCTCCG TATGAAACCT TCGAAGATTT ACGAGAGAAG
    CTCCTCATGG CTGTGGAAAA CGCCCAAGGC TTCGAGGGGG TGGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024840240.1
    CDS165..3092
    Translation

    Target ORF information:

    RefSeq Version XM_024984472.1
    Organism Bos taurus(cattle)
    Definition Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024984472.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGCGACCG GGCTCGGGGA GCCGGTCTAC GGACTTTCGG AAGAAGAGGG AGAGTCGCGT 
    ATTCTCAGAG TGAAGGTTGT TTCTGGAATT GATCTCGCCA AGAAGGACAT CTTCGGAGCC
    AGTGATCCAT ACGTGAAACT TTCACTGTAC GTAGCGGATG AGAATCGGGA ACTTGCTTTG
    GTACAAACAA AAACAATTAA AAAGACGCTG AACCCAAAGT GGAATGAAGA ATTTTATTTT
    AGAGTAAATC CATCTAATCA CAGACTCCTG TTTGAAGTAT TTGATGAAAA CAGACTGACA
    CGGGACGACT TCCTGGGACA GGTGGACATA CCCCTTAGTC ATCTCCCGAC AGAAGACCCA
    ACCATGGAGC GACCCTACAC ATTTAAGGAC TTTCTCCTCC GGCCAAGAAG TCATAAATCT
    CGGGTTAAGG GATTTTTGCG ATTGAAAATG GCCTATATGC CCAAAAACGG AGGCCAAGAT
    GAAGAGAACA GTGAGCAGCG GGATGAGATG GAGCCCGGGT GGGAAGTCGT TGACTCCAAT
    GACTCGGCTT CTCAGCACCA GGAGGAGCTG CCCCCGCCTC CGCTGCCCCC CGGGTGGGAA
    GAGAAGGTGG ACAACCTGGG CCGCACGTAC TACGTCAACC ACAACAACCG GACCACCCAG
    TGGCACCGGC CCAGCCTCAT GGACGTGTCG TCCGAGTCCG ACAGCAACAT CCGGCAGATC
    AACCAGGAGG CCGCGCATCG CCGCTTCCGC TCGCGCAGAC ATATCAGCGA GGACCTGGAG
    CCGGAGCCCA GCGAGGGCGG GGAGGGCCCC GAGCCTTGGG AGACCATTTC AGAGGAAGTG
    AACATGAGCG GAGACTCGCT CAGCCTGGCG CTGCCCCCGC CACCGGCCTC GCCCGTGTCC
    CGGACGAGCC CCCAGGAGCT GTCCGAGGAG CTGAGCAGAA GGCTGCAGGT CACTCCCGAC
    TCCAACGGCG AGCAGTTTGG GTCTTTGATT CAGAGAGAGC CGTCCTCCAG GCTGAGGTCC
    TGCAGTGTCA CCGACGCAGT CGCGGAGCAG GCTCACCTCC CGCCGCCCAG TGCCCCAGCT
    GGGAGAGCAC GTTCATCAAC TGTCACTGGT GGTGAGGCGC CAACGCCATC AGTGGCCTAT
    GTACACACCA CGCCGGGCCT GCCCTCGGGC TGGGAAGAGA GGAAAGATGC TAAGGGACGC
    ACATACTATG TCAATCATAA CAATCGAACC ACAACTTGGA CTCGGCCTAT CATGCAGCTC
    GCAGAAGACG GCGCGTCCGG ATCCGCCACA AACAGTAACA ACCACCTAAT CGAGCCTCAG
    ATCCGCCGGC CTCGTAGCCT CAGCTCGCCA ACAGTAACTT TATCCGCCCC GCTGGAGGGC
    GCCAAGGACT CACCCGTACG TCGGGCTGTG AAAGATACCC TTTCCAACCC ACAGTCCCCA
    CAGCCATCAC CTTACAACTC CCCCAAACCA CAACACAAAG TCACACAGAG CTTCCTGCCG
    CCCGGCTGGG AGATGAGGAT AGCGCCCAAC GGCCGGCCCT TCTTCATTGA CCACAACACA
    AAGACCACGA CGTGGGAAGA TCCACGCCTG AAATTTCCAG TACACATGCG GTCAAAGGCA
    TCCTTAAACC CCAATGACCT TGGCCCTCTT CCTCCTGGCT GGGAAGAAAG GATCCACTTG
    GATGGCCGGA CGTTTTACAT TGACCACAAT AGCAAAATTA CTCAGTGGGA AGACCCAAGA
    CTGCAGAACC CAGCTATTAC TGGTCCGGCT GTTCCTTACT CCAGAGAATT TAAGCAGAAA
    TATGACTACT TTAGAAAGAA ATTAAAGAAA CCTGCGGATA TTCCAAATAG GTTTGAAATG
    AAACTTCACA GAAATAACAT ATTTGAAGAG TCCTATCGGA GAATCATGTC TGTGAAAAGA
    CCAGATGTCC TGAAAGCTAG GCTGTGGATT GAGTTTGAAT CGGAGAAAGG TCTGGACTAC
    GGGGGCGTGG CCAGAGAGTG GTTCTTCCTA CTGTCCAAGG AGATGTTCAA CCCTTACTAC
    GGCCTCTTCG AGTATTCGGC AACGGACAAC TACACACTTC AGATCAATCC CAATTCAGGC
    CTCTGTAATG AAGATCATTT GTCCTATTTC ACTTTCATTG GAAGAGTTGC TGGGCTGGCA
    GTATTTCATG GGAAACTTCT AGATGGTTTC TTCATTCGAC CGTTTTATAA AATGATGCTG
    GGAAAGCAGA TAACTCTGAA TGACATGGAA TCTGTGGATA GCGAATATTA CAACTCTTTG
    AAATGGATCT TAGAAAATGA CCCAACTGAA CTGGACCTTA TGTTCTGCAT TGATGAAGAG
    AACTTTGGGC AGACATATCA AGTGGATCTG AAGCCCAACG GGTCAGAAAT AATGGTAACG
    AACGAAAACA AGAGAGAGTA CATCGACCTC GTCATCCAGT GGAGGTTCGT GAACAGGGTC
    CAGAAGCAGA TGAACGCCTT CCTGGAGGGA TTCACGGAAC TGCTCCCTAT TGATTTGATT
    AAAATCTTTG ATGAAAATGA ACTGGAGTTG CTGATGTGTG GCCTTGGCGA CGTGGATGTG
    AACGACTGGC GACAACATTC TATTTACAAG AATGGCTACT GCCCGAACCA CCCCGTGATC
    CAGTGGTTCT GGAAGGCTGT GCTGCTGATG GACGCCGAGA AGCGCATCCG GTTGCTGCAG
    TTTGTCACAG GGACGTCCCG AGTACCTATG AATGGATTTG CCGAGCTTTA TGGTTCCAAT
    GGTCCTCAGC TGTTTACAAT AGAGCAATGG GGAAGTCCTG AAAAACTGCC CAGAGCTCAC
    ACGTGCTTTA ATCGCCTTGA CTTACCTCCG TATGAAACCT TCGAAGATTT ACGAGAGAAG
    CTCCTCATGG CTGTGGAAAA CGCCCAAGGC TTCGAGGGGG TGGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa230045
    Clone ID Related Accession (Same CDS sequence) XM_024984473.1
    Accession Version XM_024984473.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2868bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGCGACCG GGCTCGGGGA GCCGGTCTAC GGACTTTCGG AAGAAGAGGG AGAGTCGCGT 
    ATTCTCAGAG TGAAGGTTGT TTCTGGAATT GATCTCGCCA AGAAGGACAT CTTCGGAGCC
    AGTGATCCAT ACGTGAAACT TTCACTGTAC GTAGCGGATG AGAATCGGGA ACTTGCTTTG
    GTACAAACAA AAACAATTAA AAAGACGCTG AACCCAAAGT GGAATGAAGA ATTTTATTTT
    AGAGTAAATC CATCTAATCA CAGACTCCTG TTTGAAGTAT TTGATGAAAA CAGACTGACA
    CGGGACGACT TCCTGGGACA GGTGGACATA CCCCTTAGTC ATCTCCCGAC AGAAGACCCA
    ACCATGGAGC GACCCTACAC ATTTAAGGAC TTTCTCCTCC GGCCAAGAAG TCATAAATCT
    CGGGTTAAGG GATTTTTGCG ATTGAAAATG GCCTATATGC CCAAAAACGG AGGCCAAGAT
    GAAGAGAACA GTGAGCAGCG GGATGAGATG GAGCCCGGGT GGGAAGTCGT TGACTCCAAT
    GACTCGGCTT CTCAGCACCA GGAGGAGCTG CCCCCGCCTC CGCTGCCCCC CGGGTGGGAA
    GAGAAGGTGG ACAACCTGGG CCGCACGTAC TACGTCAACC ACAACAACCG GACCACCCAG
    TGGCACCGGC CCAGCCTCAT GGACGTGTCG TCCGAGTCCG ACAGCAACAT CCGGCAGATC
    AACCAGGAGG CCGCGCATCG CCGCTTCCGC TCGCGCAGAC ATATCAGCGA GGACCTGGAG
    CCGGAGCCCA GCGAGGGCGG GGAGGGCCCC GAGCCTTGGG AGACCATTTC AGAGGAAGTG
    AACATGAGCG GAGACTCGCT CAGCCTGGCG CTGCCCCCGC CACCGGCCTC GCCCGTGTCC
    CGGACGAGCC CCCAGGAGCT GTCCGAGGAG CTGAGCAGAA GGCTGCAGGT CACTCCCGAC
    TCCAACGGCG AGCAGTTTGG GTCTTTGATT CAGAGAGAGC CGTCCTCCAG GCTGAGGTCC
    TGCAGTGTCA CCGACGCAGT CGCGGAGCAG GCTCACCTCC CGCCGCCATC AGTGGCCTAT
    GTACACACCA CGCCGGGCCT GCCCTCGGGC TGGGAAGAGA GGAAAGATGC TAAGGGACGC
    ACATACTATG TCAATCATAA CAATCGAACC ACAACTTGGA CTCGGCCTAT CATGCAGCTC
    GCAGAAGACG GCGCGTCCGG ATCCGCCACA AACAGTAACA ACCACCTAAT CGAGCCTCAG
    ATCCGCCGGC CTCGTAGCCT CAGCTCGCCA ACAGTAACTT TATCCGCCCC GCTGGAGGGC
    GCCAAGGACT CACCCGTACG TCGGGCTGTG AAAGATACCC TTTCCAACCC ACAGTCCCCA
    CAGCCATCAC CTTACAACTC CCCCAAACCA CAACACAAAG TCACACAGAG CTTCCTGCCG
    CCCGGCTGGG AGATGAGGAT AGCGCCCAAC GGCCGGCCCT TCTTCATTGA CCACAACACA
    AAGACCACGA CGTGGGAAGA TCCACGCCTG AAATTTCCAG TACACATGCG GTCAAAGGCA
    TCCTTAAACC CCAATGACCT TGGCCCTCTT CCTCCTGGCT GGGAAGAAAG GATCCACTTG
    GATGGCCGGA CGTTTTACAT TGACCACAAT AGCAAAATTA CTCAGTGGGA AGACCCAAGA
    CTGCAGAACC CAGCTATTAC TGGTCCGGCT GTTCCTTACT CCAGAGAATT TAAGCAGAAA
    TATGACTACT TTAGAAAGAA ATTAAAGAAA CCTGCGGATA TTCCAAATAG GTTTGAAATG
    AAACTTCACA GAAATAACAT ATTTGAAGAG TCCTATCGGA GAATCATGTC TGTGAAAAGA
    CCAGATGTCC TGAAAGCTAG GCTGTGGATT GAGTTTGAAT CGGAGAAAGG TCTGGACTAC
    GGGGGCGTGG CCAGAGAGTG GTTCTTCCTA CTGTCCAAGG AGATGTTCAA CCCTTACTAC
    GGCCTCTTCG AGTATTCGGC AACGGACAAC TACACACTTC AGATCAATCC CAATTCAGGC
    CTCTGTAATG AAGATCATTT GTCCTATTTC ACTTTCATTG GAAGAGTTGC TGGGCTGGCA
    GTATTTCATG GGAAACTTCT AGATGGTTTC TTCATTCGAC CGTTTTATAA AATGATGCTG
    GGAAAGCAGA TAACTCTGAA TGACATGGAA TCTGTGGATA GCGAATATTA CAACTCTTTG
    AAATGGATCT TAGAAAATGA CCCAACTGAA CTGGACCTTA TGTTCTGCAT TGATGAAGAG
    AACTTTGGGC AGACATATCA AGTGGATCTG AAGCCCAACG GGTCAGAAAT AATGGTAACG
    AACGAAAACA AGAGAGAGTA CATCGACCTC GTCATCCAGT GGAGGTTCGT GAACAGGGTC
    CAGAAGCAGA TGAACGCCTT CCTGGAGGGA TTCACGGAAC TGCTCCCTAT TGATTTGATT
    AAAATCTTTG ATGAAAATGA ACTGGAGTTG CTGATGTGTG GCCTTGGCGA CGTGGATGTG
    AACGACTGGC GACAACATTC TATTTACAAG AATGGCTACT GCCCGAACCA CCCCGTGATC
    CAGTGGTTCT GGAAGGCTGT GCTGCTGATG GACGCCGAGA AGCGCATCCG GTTGCTGCAG
    TTTGTCACAG GGACGTCCCG AGTACCTATG AATGGATTTG CCGAGCTTTA TGGTTCCAAT
    GGTCCTCAGC TGTTTACAAT AGAGCAATGG GGAAGTCCTG AAAAACTGCC CAGAGCTCAC
    ACGTGCTTTA ATCGCCTTGA CTTACCTCCG TATGAAACCT TCGAAGATTT ACGAGAGAAG
    CTCCTCATGG CTGTGGAAAA CGCCCAAGGC TTCGAGGGGG TGGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024840241.1
    CDS163..3030
    Translation

    Target ORF information:

    RefSeq Version XM_024984473.1
    Organism Bos taurus(cattle)
    Definition Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024984473.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGCGACCG GGCTCGGGGA GCCGGTCTAC GGACTTTCGG AAGAAGAGGG AGAGTCGCGT 
    ATTCTCAGAG TGAAGGTTGT TTCTGGAATT GATCTCGCCA AGAAGGACAT CTTCGGAGCC
    AGTGATCCAT ACGTGAAACT TTCACTGTAC GTAGCGGATG AGAATCGGGA ACTTGCTTTG
    GTACAAACAA AAACAATTAA AAAGACGCTG AACCCAAAGT GGAATGAAGA ATTTTATTTT
    AGAGTAAATC CATCTAATCA CAGACTCCTG TTTGAAGTAT TTGATGAAAA CAGACTGACA
    CGGGACGACT TCCTGGGACA GGTGGACATA CCCCTTAGTC ATCTCCCGAC AGAAGACCCA
    ACCATGGAGC GACCCTACAC ATTTAAGGAC TTTCTCCTCC GGCCAAGAAG TCATAAATCT
    CGGGTTAAGG GATTTTTGCG ATTGAAAATG GCCTATATGC CCAAAAACGG AGGCCAAGAT
    GAAGAGAACA GTGAGCAGCG GGATGAGATG GAGCCCGGGT GGGAAGTCGT TGACTCCAAT
    GACTCGGCTT CTCAGCACCA GGAGGAGCTG CCCCCGCCTC CGCTGCCCCC CGGGTGGGAA
    GAGAAGGTGG ACAACCTGGG CCGCACGTAC TACGTCAACC ACAACAACCG GACCACCCAG
    TGGCACCGGC CCAGCCTCAT GGACGTGTCG TCCGAGTCCG ACAGCAACAT CCGGCAGATC
    AACCAGGAGG CCGCGCATCG CCGCTTCCGC TCGCGCAGAC ATATCAGCGA GGACCTGGAG
    CCGGAGCCCA GCGAGGGCGG GGAGGGCCCC GAGCCTTGGG AGACCATTTC AGAGGAAGTG
    AACATGAGCG GAGACTCGCT CAGCCTGGCG CTGCCCCCGC CACCGGCCTC GCCCGTGTCC
    CGGACGAGCC CCCAGGAGCT GTCCGAGGAG CTGAGCAGAA GGCTGCAGGT CACTCCCGAC
    TCCAACGGCG AGCAGTTTGG GTCTTTGATT CAGAGAGAGC CGTCCTCCAG GCTGAGGTCC
    TGCAGTGTCA CCGACGCAGT CGCGGAGCAG GCTCACCTCC CGCCGCCATC AGTGGCCTAT
    GTACACACCA CGCCGGGCCT GCCCTCGGGC TGGGAAGAGA GGAAAGATGC TAAGGGACGC
    ACATACTATG TCAATCATAA CAATCGAACC ACAACTTGGA CTCGGCCTAT CATGCAGCTC
    GCAGAAGACG GCGCGTCCGG ATCCGCCACA AACAGTAACA ACCACCTAAT CGAGCCTCAG
    ATCCGCCGGC CTCGTAGCCT CAGCTCGCCA ACAGTAACTT TATCCGCCCC GCTGGAGGGC
    GCCAAGGACT CACCCGTACG TCGGGCTGTG AAAGATACCC TTTCCAACCC ACAGTCCCCA
    CAGCCATCAC CTTACAACTC CCCCAAACCA CAACACAAAG TCACACAGAG CTTCCTGCCG
    CCCGGCTGGG AGATGAGGAT AGCGCCCAAC GGCCGGCCCT TCTTCATTGA CCACAACACA
    AAGACCACGA CGTGGGAAGA TCCACGCCTG AAATTTCCAG TACACATGCG GTCAAAGGCA
    TCCTTAAACC CCAATGACCT TGGCCCTCTT CCTCCTGGCT GGGAAGAAAG GATCCACTTG
    GATGGCCGGA CGTTTTACAT TGACCACAAT AGCAAAATTA CTCAGTGGGA AGACCCAAGA
    CTGCAGAACC CAGCTATTAC TGGTCCGGCT GTTCCTTACT CCAGAGAATT TAAGCAGAAA
    TATGACTACT TTAGAAAGAA ATTAAAGAAA CCTGCGGATA TTCCAAATAG GTTTGAAATG
    AAACTTCACA GAAATAACAT ATTTGAAGAG TCCTATCGGA GAATCATGTC TGTGAAAAGA
    CCAGATGTCC TGAAAGCTAG GCTGTGGATT GAGTTTGAAT CGGAGAAAGG TCTGGACTAC
    GGGGGCGTGG CCAGAGAGTG GTTCTTCCTA CTGTCCAAGG AGATGTTCAA CCCTTACTAC
    GGCCTCTTCG AGTATTCGGC AACGGACAAC TACACACTTC AGATCAATCC CAATTCAGGC
    CTCTGTAATG AAGATCATTT GTCCTATTTC ACTTTCATTG GAAGAGTTGC TGGGCTGGCA
    GTATTTCATG GGAAACTTCT AGATGGTTTC TTCATTCGAC CGTTTTATAA AATGATGCTG
    GGAAAGCAGA TAACTCTGAA TGACATGGAA TCTGTGGATA GCGAATATTA CAACTCTTTG
    AAATGGATCT TAGAAAATGA CCCAACTGAA CTGGACCTTA TGTTCTGCAT TGATGAAGAG
    AACTTTGGGC AGACATATCA AGTGGATCTG AAGCCCAACG GGTCAGAAAT AATGGTAACG
    AACGAAAACA AGAGAGAGTA CATCGACCTC GTCATCCAGT GGAGGTTCGT GAACAGGGTC
    CAGAAGCAGA TGAACGCCTT CCTGGAGGGA TTCACGGAAC TGCTCCCTAT TGATTTGATT
    AAAATCTTTG ATGAAAATGA ACTGGAGTTG CTGATGTGTG GCCTTGGCGA CGTGGATGTG
    AACGACTGGC GACAACATTC TATTTACAAG AATGGCTACT GCCCGAACCA CCCCGTGATC
    CAGTGGTTCT GGAAGGCTGT GCTGCTGATG GACGCCGAGA AGCGCATCCG GTTGCTGCAG
    TTTGTCACAG GGACGTCCCG AGTACCTATG AATGGATTTG CCGAGCTTTA TGGTTCCAAT
    GGTCCTCAGC TGTTTACAAT AGAGCAATGG GGAAGTCCTG AAAAACTGCC CAGAGCTCAC
    ACGTGCTTTA ATCGCCTTGA CTTACCTCCG TATGAAACCT TCGAAGATTT ACGAGAGAAG
    CTCCTCATGG CTGTGGAAAA CGCCCAAGGC TTCGAGGGGG TGGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa230046
    Clone ID Related Accession (Same CDS sequence) XM_024984475.1
    Accession Version XM_024984475.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2628bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGCCGCCG TATGTGGTCG CTACAGAGCA CTTGAAACCC AGATGGTCCA GAGCGAGACA 
    CGGGACGACT TCCTGGGACA GGTGGACATA CCCCTTAGTC ATCTCCCGAC AGAAGACCCA
    ACCATGGAGC GACCCTACAC ATTTAAGGAC TTTCTCCTCC GGCCAAGAAG TCATAAATCT
    CGGGTTAAGG GATTTTTGCG ATTGAAAATG GCCTATATGC CCAAAAACGG AGGCCAAGAT
    GAAGAGAACA GTGAGCAGCG GGATGAGATG GAGCCCGGGT GGGAAGTCGT TGACTCCAAT
    GACTCGGCTT CTCAGCACCA GGAGGAGCTG CCCCCGCCTC CGCTGCCCCC CGGGTGGGAA
    GAGAAGGTGG ACAACCTGGG CCGCACGTAC TACGTCAACC ACAACAACCG GACCACCCAG
    TGGCACCGGC CCAGCCTCAT GGACGTGTCG TCCGAGTCCG ACAGCAACAT CCGGCAGATC
    AACCAGGAGG CCGCGCATCG CCGCTTCCGC TCGCGCAGAC ATATCAGCGA GGACCTGGAG
    CCGGAGCCCA GCGAGGGCGG GGAGGGCCCC GAGCCTTGGG AGACCATTTC AGAGGAAGTG
    AACATGAGCG GAGACTCGCT CAGCCTGGCG CTGCCCCCGC CACCGGCCTC GCCCGTGTCC
    CGGACGAGCC CCCAGGAGCT GTCCGAGGAG CTGAGCAGAA GGCTGCAGGT CACTCCCGAC
    TCCAACGGCG AGCAGTTTGG GTCTTTGATT CAGAGAGAGC CGTCCTCCAG GCTGAGGTCC
    TGCAGTGTCA CCGACGCAGT CGCGGAGCAG GCTCACCTCC CGCCGCCATC AGTGGCCTAT
    GTACACACCA CGCCGGGCCT GCCCTCGGGC TGGGAAGAGA GGAAAGATGC TAAGGGACGC
    ACATACTATG TCAATCATAA CAATCGAACC ACAACTTGGA CTCGGCCTAT CATGCAGCTC
    GCAGAAGACG GCGCGTCCGG ATCCGCCACA AACAGTAACA ACCACCTAAT CGAGCCTCAG
    ATCCGCCGGC CTCGTAGCCT CAGCTCGCCA ACAGTAACTT TATCCGCCCC GCTGGAGGGC
    GCCAAGGACT CACCCGTACG TCGGGCTGTG AAAGATACCC TTTCCAACCC ACAGTCCCCA
    CAGCCATCAC CTTACAACTC CCCCAAACCA CAACACAAAG TCACACAGAG CTTCCTGCCG
    CCCGGCTGGG AGATGAGGAT AGCGCCCAAC GGCCGGCCCT TCTTCATTGA CCACAACACA
    AAGACCACGA CGTGGGAAGA TCCACGCCTG AAATTTCCAG TACACATGCG GTCAAAGGCA
    TCCTTAAACC CCAATGACCT TGGCCCTCTT CCTCCTGGCT GGGAAGAAAG GATCCACTTG
    GATGGCCGGA CGTTTTACAT TGACCACAAT AGCAAAATTA CTCAGTGGGA AGACCCAAGA
    CTGCAGAACC CAGCTATTAC TGGTCCGGCT GTTCCTTACT CCAGAGAATT TAAGCAGAAA
    TATGACTACT TTAGAAAGAA ATTAAAGAAA CCTGCGGATA TTCCAAATAG GTTTGAAATG
    AAACTTCACA GAAATAACAT ATTTGAAGAG TCCTATCGGA GAATCATGTC TGTGAAAAGA
    CCAGATGTCC TGAAAGCTAG GCTGTGGATT GAGTTTGAAT CGGAGAAAGG TCTGGACTAC
    GGGGGCGTGG CCAGAGAGTG GTTCTTCCTA CTGTCCAAGG AGATGTTCAA CCCTTACTAC
    GGCCTCTTCG AGTATTCGGC AACGGACAAC TACACACTTC AGATCAATCC CAATTCAGGC
    CTCTGTAATG AAGATCATTT GTCCTATTTC ACTTTCATTG GAAGAGTTGC TGGGCTGGCA
    GTATTTCATG GGAAACTTCT AGATGGTTTC TTCATTCGAC CGTTTTATAA AATGATGCTG
    GGAAAGCAGA TAACTCTGAA TGACATGGAA TCTGTGGATA GCGAATATTA CAACTCTTTG
    AAATGGATCT TAGAAAATGA CCCAACTGAA CTGGACCTTA TGTTCTGCAT TGATGAAGAG
    AACTTTGGGC AGACATATCA AGTGGATCTG AAGCCCAACG GGTCAGAAAT AATGGTAACG
    AACGAAAACA AGAGAGAGTA CATCGACCTC GTCATCCAGT GGAGGTTCGT GAACAGGGTC
    CAGAAGCAGA TGAACGCCTT CCTGGAGGGA TTCACGGAAC TGCTCCCTAT TGATTTGATT
    AAAATCTTTG ATGAAAATGA ACTGGAGTTG CTGATGTGTG GCCTTGGCGA CGTGGATGTG
    AACGACTGGC GACAACATTC TATTTACAAG AATGGCTACT GCCCGAACCA CCCCGTGATC
    CAGTGGTTCT GGAAGGCTGT GCTGCTGATG GACGCCGAGA AGCGCATCCG GTTGCTGCAG
    TTTGTCACAG GGACGTCCCG AGTACCTATG AATGGATTTG CCGAGCTTTA TGGTTCCAAT
    GGTCCTCAGC TGTTTACAAT AGAGCAATGG GGAAGTCCTG AAAAACTGCC CAGAGCTCAC
    ACGTGCTTTA ATCGCCTTGA CTTACCTCCG TATGAAACCT TCGAAGATTT ACGAGAGAAG
    CTCCTCATGG CTGTGGAAAA CGCCCAAGGC TTCGAGGGGG TGGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024840243.1
    CDS27..2654
    Translation

    Target ORF information:

    RefSeq Version XM_024984475.1
    Organism Bos taurus(cattle)
    Definition Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024984475.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGCCGCCG TATGTGGTCG CTACAGAGCA CTTGAAACCC AGATGGTCCA GAGCGAGACA 
    CGGGACGACT TCCTGGGACA GGTGGACATA CCCCTTAGTC ATCTCCCGAC AGAAGACCCA
    ACCATGGAGC GACCCTACAC ATTTAAGGAC TTTCTCCTCC GGCCAAGAAG TCATAAATCT
    CGGGTTAAGG GATTTTTGCG ATTGAAAATG GCCTATATGC CCAAAAACGG AGGCCAAGAT
    GAAGAGAACA GTGAGCAGCG GGATGAGATG GAGCCCGGGT GGGAAGTCGT TGACTCCAAT
    GACTCGGCTT CTCAGCACCA GGAGGAGCTG CCCCCGCCTC CGCTGCCCCC CGGGTGGGAA
    GAGAAGGTGG ACAACCTGGG CCGCACGTAC TACGTCAACC ACAACAACCG GACCACCCAG
    TGGCACCGGC CCAGCCTCAT GGACGTGTCG TCCGAGTCCG ACAGCAACAT CCGGCAGATC
    AACCAGGAGG CCGCGCATCG CCGCTTCCGC TCGCGCAGAC ATATCAGCGA GGACCTGGAG
    CCGGAGCCCA GCGAGGGCGG GGAGGGCCCC GAGCCTTGGG AGACCATTTC AGAGGAAGTG
    AACATGAGCG GAGACTCGCT CAGCCTGGCG CTGCCCCCGC CACCGGCCTC GCCCGTGTCC
    CGGACGAGCC CCCAGGAGCT GTCCGAGGAG CTGAGCAGAA GGCTGCAGGT CACTCCCGAC
    TCCAACGGCG AGCAGTTTGG GTCTTTGATT CAGAGAGAGC CGTCCTCCAG GCTGAGGTCC
    TGCAGTGTCA CCGACGCAGT CGCGGAGCAG GCTCACCTCC CGCCGCCATC AGTGGCCTAT
    GTACACACCA CGCCGGGCCT GCCCTCGGGC TGGGAAGAGA GGAAAGATGC TAAGGGACGC
    ACATACTATG TCAATCATAA CAATCGAACC ACAACTTGGA CTCGGCCTAT CATGCAGCTC
    GCAGAAGACG GCGCGTCCGG ATCCGCCACA AACAGTAACA ACCACCTAAT CGAGCCTCAG
    ATCCGCCGGC CTCGTAGCCT CAGCTCGCCA ACAGTAACTT TATCCGCCCC GCTGGAGGGC
    GCCAAGGACT CACCCGTACG TCGGGCTGTG AAAGATACCC TTTCCAACCC ACAGTCCCCA
    CAGCCATCAC CTTACAACTC CCCCAAACCA CAACACAAAG TCACACAGAG CTTCCTGCCG
    CCCGGCTGGG AGATGAGGAT AGCGCCCAAC GGCCGGCCCT TCTTCATTGA CCACAACACA
    AAGACCACGA CGTGGGAAGA TCCACGCCTG AAATTTCCAG TACACATGCG GTCAAAGGCA
    TCCTTAAACC CCAATGACCT TGGCCCTCTT CCTCCTGGCT GGGAAGAAAG GATCCACTTG
    GATGGCCGGA CGTTTTACAT TGACCACAAT AGCAAAATTA CTCAGTGGGA AGACCCAAGA
    CTGCAGAACC CAGCTATTAC TGGTCCGGCT GTTCCTTACT CCAGAGAATT TAAGCAGAAA
    TATGACTACT TTAGAAAGAA ATTAAAGAAA CCTGCGGATA TTCCAAATAG GTTTGAAATG
    AAACTTCACA GAAATAACAT ATTTGAAGAG TCCTATCGGA GAATCATGTC TGTGAAAAGA
    CCAGATGTCC TGAAAGCTAG GCTGTGGATT GAGTTTGAAT CGGAGAAAGG TCTGGACTAC
    GGGGGCGTGG CCAGAGAGTG GTTCTTCCTA CTGTCCAAGG AGATGTTCAA CCCTTACTAC
    GGCCTCTTCG AGTATTCGGC AACGGACAAC TACACACTTC AGATCAATCC CAATTCAGGC
    CTCTGTAATG AAGATCATTT GTCCTATTTC ACTTTCATTG GAAGAGTTGC TGGGCTGGCA
    GTATTTCATG GGAAACTTCT AGATGGTTTC TTCATTCGAC CGTTTTATAA AATGATGCTG
    GGAAAGCAGA TAACTCTGAA TGACATGGAA TCTGTGGATA GCGAATATTA CAACTCTTTG
    AAATGGATCT TAGAAAATGA CCCAACTGAA CTGGACCTTA TGTTCTGCAT TGATGAAGAG
    AACTTTGGGC AGACATATCA AGTGGATCTG AAGCCCAACG GGTCAGAAAT AATGGTAACG
    AACGAAAACA AGAGAGAGTA CATCGACCTC GTCATCCAGT GGAGGTTCGT GAACAGGGTC
    CAGAAGCAGA TGAACGCCTT CCTGGAGGGA TTCACGGAAC TGCTCCCTAT TGATTTGATT
    AAAATCTTTG ATGAAAATGA ACTGGAGTTG CTGATGTGTG GCCTTGGCGA CGTGGATGTG
    AACGACTGGC GACAACATTC TATTTACAAG AATGGCTACT GCCCGAACCA CCCCGTGATC
    CAGTGGTTCT GGAAGGCTGT GCTGCTGATG GACGCCGAGA AGCGCATCCG GTTGCTGCAG
    TTTGTCACAG GGACGTCCCG AGTACCTATG AATGGATTTG CCGAGCTTTA TGGTTCCAAT
    GGTCCTCAGC TGTTTACAAT AGAGCAATGG GGAAGTCCTG AAAAACTGCC CAGAGCTCAC
    ACGTGCTTTA ATCGCCTTGA CTTACCTCCG TATGAAACCT TCGAAGATTT ACGAGAGAAG
    CTCCTCATGG CTGTGGAAAA CGCCCAAGGC TTCGAGGGGG TGGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa230047
    Clone ID Related Accession (Same CDS sequence) XM_024984486.1
    Accession Version XM_024984486.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2376bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGCCGCCG TATGTGGTCG CTACAGAGCA CTTGAAACCC AGATGGTCCA GAGCGAGACA 
    CGGGACGACT TCCTGGGACA GGTGGACATA CCCCTTAGTC ATCTCCCGAC AGAAGACCCA
    ACCATGGAGC GACCCTACAC ATTTAAGGAC TTTCTCCTCC GGCCAAGAAG TCATAAATCT
    CGGGTTAAGG GATTTTTGCG ATTGAAAATG GCCTATATGC CCAAAAACGG AGGCCAAGAT
    GAAGAGAACA GTGAGCAGCG GGATGAGATG GAGCCCGGGT GGGAAGTCGT TGACTCCAAT
    GACTCGGCTT CTCAGCACCA GGAGGAGCTG CCCCCGCCTC CGCTGCCCCC CGGGTGGGAA
    GAGAAGGTGG ACAACCTGGG CCGCACGTAC TACGTCAACC ACAACAACCG GACCACCCAG
    TGGCACCGGC CCAGCCTCAT GGACGTGTCG TCCGAGTCCG ACAGCAACAT CCGGCAGATC
    AACCAGGAGG CCGCGCATCG CCGCTTCCGC TCGCGCAGAC ATATCAGCGA GGACCTGGAG
    CCGGAGCCCA GCGAGGGCGG GGAGGGCCCC GAGCCTTGGG AGACCATTTC AGAGGAAGTG
    AACATGAGCG GAGACTCGCT CAGCCTGGCG CTGCCCCCGC CACCGGCCTC GCCCGTGTCC
    CGGACGAGCC CCCAGGAGCT GTCCGAGGAG CTGAGCAGAA GGCTGCAGGT CACTCCCGAC
    TCCAACGGCG AGCAGTTTGG GTCTTTGATT CAGAGAGAGC CGTCCTCCAG GCTGAGGTCC
    TGCAGTGTCA CCGACGCAGT CGCGGAGCAG GCTCACCTCC CGCCGGGCGC CAAGGACTCA
    CCCGTACGTC GGGCTGTGAA AGATACCCTT TCCAACCCAC AGTCCCCACA GCCATCACCT
    TACAACTCCC CCAAACCACA ACACAAAGTC ACACAGAGCT TCCTGCCGCC CGGCTGGGAG
    ATGAGGATAG CGCCCAACGG CCGGCCCTTC TTCATTGACC ACAACACAAA GACCACGACG
    TGGGAAGATC CACGCCTGAA ATTTCCAGTA CACATGCGGT CAAAGGCATC CTTAAACCCC
    AATGACCTTG GCCCTCTTCC TCCTGGCTGG GAAGAAAGGA TCCACTTGGA TGGCCGGACG
    TTTTACATTG ACCACAATAG CAAAATTACT CAGTGGGAAG ACCCAAGACT GCAGAACCCA
    GCTATTACTG GTCCGGCTGT TCCTTACTCC AGAGAATTTA AGCAGAAATA TGACTACTTT
    AGAAAGAAAT TAAAGAAACC TGCGGATATT CCAAATAGGT TTGAAATGAA ACTTCACAGA
    AATAACATAT TTGAAGAGTC CTATCGGAGA ATCATGTCTG TGAAAAGACC AGATGTCCTG
    AAAGCTAGGC TGTGGATTGA GTTTGAATCG GAGAAAGGTC TGGACTACGG GGGCGTGGCC
    AGAGAGTGGT TCTTCCTACT GTCCAAGGAG ATGTTCAACC CTTACTACGG CCTCTTCGAG
    TATTCGGCAA CGGACAACTA CACACTTCAG ATCAATCCCA ATTCAGGCCT CTGTAATGAA
    GATCATTTGT CCTATTTCAC TTTCATTGGA AGAGTTGCTG GGCTGGCAGT ATTTCATGGG
    AAACTTCTAG ATGGTTTCTT CATTCGACCG TTTTATAAAA TGATGCTGGG AAAGCAGATA
    ACTCTGAATG ACATGGAATC TGTGGATAGC GAATATTACA ACTCTTTGAA ATGGATCTTA
    GAAAATGACC CAACTGAACT GGACCTTATG TTCTGCATTG ATGAAGAGAA CTTTGGGCAG
    ACATATCAAG TGGATCTGAA GCCCAACGGG TCAGAAATAA TGGTAACGAA CGAAAACAAG
    AGAGAGTACA TCGACCTCGT CATCCAGTGG AGGTTCGTGA ACAGGGTCCA GAAGCAGATG
    AACGCCTTCC TGGAGGGATT CACGGAACTG CTCCCTATTG ATTTGATTAA AATCTTTGAT
    GAAAATGAAC TGGAGTTGCT GATGTGTGGC CTTGGCGACG TGGATGTGAA CGACTGGCGA
    CAACATTCTA TTTACAAGAA TGGCTACTGC CCGAACCACC CCGTGATCCA GTGGTTCTGG
    AAGGCTGTGC TGCTGATGGA CGCCGAGAAG CGCATCCGGT TGCTGCAGTT TGTCACAGGG
    ACGTCCCGAG TACCTATGAA TGGATTTGCC GAGCTTTATG GTTCCAATGG TCCTCAGCTG
    TTTACAATAG AGCAATGGGG AAGTCCTGAA AAACTGCCCA GAGCTCACAC GTGCTTTAAT
    CGCCTTGACT TACCTCCGTA TGAAACCTTC GAAGATTTAC GAGAGAAGCT CCTCATGGCT
    GTGGAAAACG CCCAAGGCTT CGAGGGGGTG GATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024840254.1
    CDS21..2396
    Translation

    Target ORF information:

    RefSeq Version XM_024984486.1
    Organism Bos taurus(cattle)
    Definition Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X26, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024984486.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGCCGCCG TATGTGGTCG CTACAGAGCA CTTGAAACCC AGATGGTCCA GAGCGAGACA 
    CGGGACGACT TCCTGGGACA GGTGGACATA CCCCTTAGTC ATCTCCCGAC AGAAGACCCA
    ACCATGGAGC GACCCTACAC ATTTAAGGAC TTTCTCCTCC GGCCAAGAAG TCATAAATCT
    CGGGTTAAGG GATTTTTGCG ATTGAAAATG GCCTATATGC CCAAAAACGG AGGCCAAGAT
    GAAGAGAACA GTGAGCAGCG GGATGAGATG GAGCCCGGGT GGGAAGTCGT TGACTCCAAT
    GACTCGGCTT CTCAGCACCA GGAGGAGCTG CCCCCGCCTC CGCTGCCCCC CGGGTGGGAA
    GAGAAGGTGG ACAACCTGGG CCGCACGTAC TACGTCAACC ACAACAACCG GACCACCCAG
    TGGCACCGGC CCAGCCTCAT GGACGTGTCG TCCGAGTCCG ACAGCAACAT CCGGCAGATC
    AACCAGGAGG CCGCGCATCG CCGCTTCCGC TCGCGCAGAC ATATCAGCGA GGACCTGGAG
    CCGGAGCCCA GCGAGGGCGG GGAGGGCCCC GAGCCTTGGG AGACCATTTC AGAGGAAGTG
    AACATGAGCG GAGACTCGCT CAGCCTGGCG CTGCCCCCGC CACCGGCCTC GCCCGTGTCC
    CGGACGAGCC CCCAGGAGCT GTCCGAGGAG CTGAGCAGAA GGCTGCAGGT CACTCCCGAC
    TCCAACGGCG AGCAGTTTGG GTCTTTGATT CAGAGAGAGC CGTCCTCCAG GCTGAGGTCC
    TGCAGTGTCA CCGACGCAGT CGCGGAGCAG GCTCACCTCC CGCCGGGCGC CAAGGACTCA
    CCCGTACGTC GGGCTGTGAA AGATACCCTT TCCAACCCAC AGTCCCCACA GCCATCACCT
    TACAACTCCC CCAAACCACA ACACAAAGTC ACACAGAGCT TCCTGCCGCC CGGCTGGGAG
    ATGAGGATAG CGCCCAACGG CCGGCCCTTC TTCATTGACC ACAACACAAA GACCACGACG
    TGGGAAGATC CACGCCTGAA ATTTCCAGTA CACATGCGGT CAAAGGCATC CTTAAACCCC
    AATGACCTTG GCCCTCTTCC TCCTGGCTGG GAAGAAAGGA TCCACTTGGA TGGCCGGACG
    TTTTACATTG ACCACAATAG CAAAATTACT CAGTGGGAAG ACCCAAGACT GCAGAACCCA
    GCTATTACTG GTCCGGCTGT TCCTTACTCC AGAGAATTTA AGCAGAAATA TGACTACTTT
    AGAAAGAAAT TAAAGAAACC TGCGGATATT CCAAATAGGT TTGAAATGAA ACTTCACAGA
    AATAACATAT TTGAAGAGTC CTATCGGAGA ATCATGTCTG TGAAAAGACC AGATGTCCTG
    AAAGCTAGGC TGTGGATTGA GTTTGAATCG GAGAAAGGTC TGGACTACGG GGGCGTGGCC
    AGAGAGTGGT TCTTCCTACT GTCCAAGGAG ATGTTCAACC CTTACTACGG CCTCTTCGAG
    TATTCGGCAA CGGACAACTA CACACTTCAG ATCAATCCCA ATTCAGGCCT CTGTAATGAA
    GATCATTTGT CCTATTTCAC TTTCATTGGA AGAGTTGCTG GGCTGGCAGT ATTTCATGGG
    AAACTTCTAG ATGGTTTCTT CATTCGACCG TTTTATAAAA TGATGCTGGG AAAGCAGATA
    ACTCTGAATG ACATGGAATC TGTGGATAGC GAATATTACA ACTCTTTGAA ATGGATCTTA
    GAAAATGACC CAACTGAACT GGACCTTATG TTCTGCATTG ATGAAGAGAA CTTTGGGCAG
    ACATATCAAG TGGATCTGAA GCCCAACGGG TCAGAAATAA TGGTAACGAA CGAAAACAAG
    AGAGAGTACA TCGACCTCGT CATCCAGTGG AGGTTCGTGA ACAGGGTCCA GAAGCAGATG
    AACGCCTTCC TGGAGGGATT CACGGAACTG CTCCCTATTG ATTTGATTAA AATCTTTGAT
    GAAAATGAAC TGGAGTTGCT GATGTGTGGC CTTGGCGACG TGGATGTGAA CGACTGGCGA
    CAACATTCTA TTTACAAGAA TGGCTACTGC CCGAACCACC CCGTGATCCA GTGGTTCTGG
    AAGGCTGTGC TGCTGATGGA CGCCGAGAAG CGCATCCGGT TGCTGCAGTT TGTCACAGGG
    ACGTCCCGAG TACCTATGAA TGGATTTGCC GAGCTTTATG GTTCCAATGG TCCTCAGCTG
    TTTACAATAG AGCAATGGGG AAGTCCTGAA AAACTGCCCA GAGCTCACAC GTGCTTTAAT
    CGCCTTGACT TACCTCCGTA TGAAACCTTC GAAGATTTAC GAGAGAAGCT CCTCATGGCT
    GTGGAAAACG CCCAAGGCTT CGAGGGGGTG GATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88365
    Clone ID Related Accession (Same CDS sequence) XM_002697776.5 , XM_002697776.6 , XM_005199753.3 , XM_015469187.1 , XM_015460158.1 , XM_024984480.1 , XM_024984481.1
    Accession Version XM_002697776.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2565bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104569.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002697776.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGCCCAGTGC CCCAGCTGGG
    AGAGCACGTT CATCAACTGT CACTGGTGGT GAGGCGCCAA CGCCATCAGT GGCCTATGTA
    CACACCACGC CGGGCCTGCC CTCGGGCTGG GAAGAGAGGA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCTATCAT GCAGCTCGCA
    GAAGACGGCG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CCGCCCCGCT GGAGGGCGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GATACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAGA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA CAACACAAAG
    ACCACGACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ACATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGCTGGG AAGAAAGGAT CCACTTGGAT
    GGCCGGACGT TTTACATTGA CCACAATAGC AAAATTACTC AGTGGGAAGA CCCAAGACTG
    CAGAACCCAG CTATTACTGG TCCGGCTGTT CCTTACTCCA GAGAATTTAA GCAGAAATAT
    GACTACTTTA GAAAGAAATT AAAGAAACCT GCGGATATTC CAAATAGGTT TGAAATGAAA
    CTTCACAGAA ATAACATATT TGAAGAGTCC TATCGGAGAA TCATGTCTGT GAAAAGACCA
    GATGTCCTGA AAGCTAGGCT GTGGATTGAG TTTGAATCGG AGAAAGGTCT GGACTACGGG
    GGCGTGGCCA GAGAGTGGTT CTTCCTACTG TCCAAGGAGA TGTTCAACCC TTACTACGGC
    CTCTTCGAGT ATTCGGCAAC GGACAACTAC ACACTTCAGA TCAATCCCAA TTCAGGCCTC
    TGTAATGAAG ATCATTTGTC CTATTTCACT TTCATTGGAA GAGTTGCTGG GCTGGCAGTA
    TTTCATGGGA AACTTCTAGA TGGTTTCTTC ATTCGACCGT TTTATAAAAT GATGCTGGGA
    AAGCAGATAA CTCTGAATGA CATGGAATCT GTGGATAGCG AATATTACAA CTCTTTGAAA
    TGGATCTTAG AAAATGACCC AACTGAACTG GACCTTATGT TCTGCATTGA TGAAGAGAAC
    TTTGGGCAGA CATATCAAGT GGATCTGAAG CCCAACGGGT CAGAAATAAT GGTAACGAAC
    GAAAACAAGA GAGAGTACAT CGACCTCGTC ATCCAGTGGA GGTTCGTGAA CAGGGTCCAG
    AAGCAGATGA ACGCCTTCCT GGAGGGATTC ACGGAACTGC TCCCTATTGA TTTGATTAAA
    ATCTTTGATG AAAATGAACT GGAGTTGCTG ATGTGTGGCC TTGGCGACGT GGATGTGAAC
    GACTGGCGAC AACATTCTAT TTACAAGAAT GGCTACTGCC CGAACCACCC CGTGATCCAG
    TGGTTCTGGA AGGCTGTGCT GCTGATGGAC GCCGAGAAGC GCATCCGGTT GCTGCAGTTT
    GTCACAGGGA CGTCCCGAGT ACCTATGAAT GGATTTGCCG AGCTTTATGG TTCCAATGGT
    CCTCAGCTGT TTACAATAGA GCAATGGGGA AGTCCTGAAA AACTGCCCAG AGCTCACACG
    TGCTTTAATC GCCTTGACTT ACCTCCGTAT GAAACCTTCG AAGATTTACG AGAGAAGCTC
    CTCATGGCTG TGGAAAACGC CCAAGGCTTC GAGGGGGTGG ATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002697822.5
    CDS554..3118
    Translation

    Target ORF information:

    RefSeq Version XM_002697776.5
    Organism Bos taurus(cattle)
    Definition Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002697776.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGCCCAGTGC CCCAGCTGGG
    AGAGCACGTT CATCAACTGT CACTGGTGGT GAGGCGCCAA CGCCATCAGT GGCCTATGTA
    CACACCACGC CGGGCCTGCC CTCGGGCTGG GAAGAGAGGA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCTATCAT GCAGCTCGCA
    GAAGACGGCG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CCGCCCCGCT GGAGGGCGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GATACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAGA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA CAACACAAAG
    ACCACGACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ACATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGCTGGG AAGAAAGGAT CCACTTGGAT
    GGCCGGACGT TTTACATTGA CCACAATAGC AAAATTACTC AGTGGGAAGA CCCAAGACTG
    CAGAACCCAG CTATTACTGG TCCGGCTGTT CCTTACTCCA GAGAATTTAA GCAGAAATAT
    GACTACTTTA GAAAGAAATT AAAGAAACCT GCGGATATTC CAAATAGGTT TGAAATGAAA
    CTTCACAGAA ATAACATATT TGAAGAGTCC TATCGGAGAA TCATGTCTGT GAAAAGACCA
    GATGTCCTGA AAGCTAGGCT GTGGATTGAG TTTGAATCGG AGAAAGGTCT GGACTACGGG
    GGCGTGGCCA GAGAGTGGTT CTTCCTACTG TCCAAGGAGA TGTTCAACCC TTACTACGGC
    CTCTTCGAGT ATTCGGCAAC GGACAACTAC ACACTTCAGA TCAATCCCAA TTCAGGCCTC
    TGTAATGAAG ATCATTTGTC CTATTTCACT TTCATTGGAA GAGTTGCTGG GCTGGCAGTA
    TTTCATGGGA AACTTCTAGA TGGTTTCTTC ATTCGACCGT TTTATAAAAT GATGCTGGGA
    AAGCAGATAA CTCTGAATGA CATGGAATCT GTGGATAGCG AATATTACAA CTCTTTGAAA
    TGGATCTTAG AAAATGACCC AACTGAACTG GACCTTATGT TCTGCATTGA TGAAGAGAAC
    TTTGGGCAGA CATATCAAGT GGATCTGAAG CCCAACGGGT CAGAAATAAT GGTAACGAAC
    GAAAACAAGA GAGAGTACAT CGACCTCGTC ATCCAGTGGA GGTTCGTGAA CAGGGTCCAG
    AAGCAGATGA ACGCCTTCCT GGAGGGATTC ACGGAACTGC TCCCTATTGA TTTGATTAAA
    ATCTTTGATG AAAATGAACT GGAGTTGCTG ATGTGTGGCC TTGGCGACGT GGATGTGAAC
    GACTGGCGAC AACATTCTAT TTACAAGAAT GGCTACTGCC CGAACCACCC CGTGATCCAG
    TGGTTCTGGA AGGCTGTGCT GCTGATGGAC GCCGAGAAGC GCATCCGGTT GCTGCAGTTT
    GTCACAGGGA CGTCCCGAGT ACCTATGAAT GGATTTGCCG AGCTTTATGG TTCCAATGGT
    CCTCAGCTGT TTACAATAGA GCAATGGGGA AGTCCTGAAA AACTGCCCAG AGCTCACACG
    TGCTTTAATC GCCTTGACTT ACCTCCGTAT GAAACCTTCG AAGATTTACG AGAGAAGCTC
    CTCATGGCTG TGGAAAACGC CCAAGGCTTC GAGGGGGTGG ATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88365
    Clone ID Related Accession (Same CDS sequence) XM_002697776.5 , XM_002697776.6 , XM_005199753.3 , XM_015469187.1 , XM_015460158.1 , XM_024984480.1 , XM_024984481.1
    Accession Version XM_002697776.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2565bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002697776.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGCCCAGTGC CCCAGCTGGG
    AGAGCACGTT CATCAACTGT CACTGGTGGT GAGGCGCCAA CGCCATCAGT GGCCTATGTA
    CACACCACGC CGGGCCTGCC CTCGGGCTGG GAAGAGAGGA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCTATCAT GCAGCTCGCA
    GAAGACGGCG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CCGCCCCGCT GGAGGGCGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GATACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAGA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA CAACACAAAG
    ACCACGACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ACATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGCTGGG AAGAAAGGAT CCACTTGGAT
    GGCCGGACGT TTTACATTGA CCACAATAGC AAAATTACTC AGTGGGAAGA CCCAAGACTG
    CAGAACCCAG CTATTACTGG TCCGGCTGTT CCTTACTCCA GAGAATTTAA GCAGAAATAT
    GACTACTTTA GAAAGAAATT AAAGAAACCT GCGGATATTC CAAATAGGTT TGAAATGAAA
    CTTCACAGAA ATAACATATT TGAAGAGTCC TATCGGAGAA TCATGTCTGT GAAAAGACCA
    GATGTCCTGA AAGCTAGGCT GTGGATTGAG TTTGAATCGG AGAAAGGTCT GGACTACGGG
    GGCGTGGCCA GAGAGTGGTT CTTCCTACTG TCCAAGGAGA TGTTCAACCC TTACTACGGC
    CTCTTCGAGT ATTCGGCAAC GGACAACTAC ACACTTCAGA TCAATCCCAA TTCAGGCCTC
    TGTAATGAAG ATCATTTGTC CTATTTCACT TTCATTGGAA GAGTTGCTGG GCTGGCAGTA
    TTTCATGGGA AACTTCTAGA TGGTTTCTTC ATTCGACCGT TTTATAAAAT GATGCTGGGA
    AAGCAGATAA CTCTGAATGA CATGGAATCT GTGGATAGCG AATATTACAA CTCTTTGAAA
    TGGATCTTAG AAAATGACCC AACTGAACTG GACCTTATGT TCTGCATTGA TGAAGAGAAC
    TTTGGGCAGA CATATCAAGT GGATCTGAAG CCCAACGGGT CAGAAATAAT GGTAACGAAC
    GAAAACAAGA GAGAGTACAT CGACCTCGTC ATCCAGTGGA GGTTCGTGAA CAGGGTCCAG
    AAGCAGATGA ACGCCTTCCT GGAGGGATTC ACGGAACTGC TCCCTATTGA TTTGATTAAA
    ATCTTTGATG AAAATGAACT GGAGTTGCTG ATGTGTGGCC TTGGCGACGT GGATGTGAAC
    GACTGGCGAC AACATTCTAT TTACAAGAAT GGCTACTGCC CGAACCACCC CGTGATCCAG
    TGGTTCTGGA AGGCTGTGCT GCTGATGGAC GCCGAGAAGC GCATCCGGTT GCTGCAGTTT
    GTCACAGGGA CGTCCCGAGT ACCTATGAAT GGATTTGCCG AGCTTTATGG TTCCAATGGT
    CCTCAGCTGT TTACAATAGA GCAATGGGGA AGTCCTGAAA AACTGCCCAG AGCTCACACG
    TGCTTTAATC GCCTTGACTT ACCTCCGTAT GAAACCTTCG AAGATTTACG AGAGAAGCTC
    CTCATGGCTG TGGAAAACGC CCAAGGCTTC GAGGGGGTGG ATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002697822.5
    CDS552..3116
    Translation

    Target ORF information:

    RefSeq Version XM_002697776.6
    Organism Bos taurus(cattle)
    Definition Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002697776.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGCCCAGTGC CCCAGCTGGG
    AGAGCACGTT CATCAACTGT CACTGGTGGT GAGGCGCCAA CGCCATCAGT GGCCTATGTA
    CACACCACGC CGGGCCTGCC CTCGGGCTGG GAAGAGAGGA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCTATCAT GCAGCTCGCA
    GAAGACGGCG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CCGCCCCGCT GGAGGGCGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GATACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAGA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA CAACACAAAG
    ACCACGACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ACATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGCTGGG AAGAAAGGAT CCACTTGGAT
    GGCCGGACGT TTTACATTGA CCACAATAGC AAAATTACTC AGTGGGAAGA CCCAAGACTG
    CAGAACCCAG CTATTACTGG TCCGGCTGTT CCTTACTCCA GAGAATTTAA GCAGAAATAT
    GACTACTTTA GAAAGAAATT AAAGAAACCT GCGGATATTC CAAATAGGTT TGAAATGAAA
    CTTCACAGAA ATAACATATT TGAAGAGTCC TATCGGAGAA TCATGTCTGT GAAAAGACCA
    GATGTCCTGA AAGCTAGGCT GTGGATTGAG TTTGAATCGG AGAAAGGTCT GGACTACGGG
    GGCGTGGCCA GAGAGTGGTT CTTCCTACTG TCCAAGGAGA TGTTCAACCC TTACTACGGC
    CTCTTCGAGT ATTCGGCAAC GGACAACTAC ACACTTCAGA TCAATCCCAA TTCAGGCCTC
    TGTAATGAAG ATCATTTGTC CTATTTCACT TTCATTGGAA GAGTTGCTGG GCTGGCAGTA
    TTTCATGGGA AACTTCTAGA TGGTTTCTTC ATTCGACCGT TTTATAAAAT GATGCTGGGA
    AAGCAGATAA CTCTGAATGA CATGGAATCT GTGGATAGCG AATATTACAA CTCTTTGAAA
    TGGATCTTAG AAAATGACCC AACTGAACTG GACCTTATGT TCTGCATTGA TGAAGAGAAC
    TTTGGGCAGA CATATCAAGT GGATCTGAAG CCCAACGGGT CAGAAATAAT GGTAACGAAC
    GAAAACAAGA GAGAGTACAT CGACCTCGTC ATCCAGTGGA GGTTCGTGAA CAGGGTCCAG
    AAGCAGATGA ACGCCTTCCT GGAGGGATTC ACGGAACTGC TCCCTATTGA TTTGATTAAA
    ATCTTTGATG AAAATGAACT GGAGTTGCTG ATGTGTGGCC TTGGCGACGT GGATGTGAAC
    GACTGGCGAC AACATTCTAT TTACAAGAAT GGCTACTGCC CGAACCACCC CGTGATCCAG
    TGGTTCTGGA AGGCTGTGCT GCTGATGGAC GCCGAGAAGC GCATCCGGTT GCTGCAGTTT
    GTCACAGGGA CGTCCCGAGT ACCTATGAAT GGATTTGCCG AGCTTTATGG TTCCAATGGT
    CCTCAGCTGT TTACAATAGA GCAATGGGGA AGTCCTGAAA AACTGCCCAG AGCTCACACG
    TGCTTTAATC GCCTTGACTT ACCTCCGTAT GAAACCTTCG AAGATTTACG AGAGAAGCTC
    CTCATGGCTG TGGAAAACGC CCAAGGCTTC GAGGGGGTGG ATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88365
    Clone ID Related Accession (Same CDS sequence) XM_002697776.5 , XM_002697776.6 , XM_005199753.3 , XM_015469187.1 , XM_015460158.1 , XM_024984480.1 , XM_024984481.1
    Accession Version XM_005199753.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2565bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646636.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005199753.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGCCCAGTGC CCCAGCTGGG
    AGAGCACGTT CATCAACTGT CACTGGTGGT GAGGCGCCAA CGCCATCAGT GGCCTATGTA
    CACACCACGC CGGGCCTGCC CTCGGGCTGG GAAGAGAGGA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCTATCAT GCAGCTCGCA
    GAAGACGGCG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CCGCCCCGCT GGAGGGCGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GATACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAGA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA CAACACAAAG
    ACCACGACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ACATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGCTGGG AAGAAAGGAT CCACTTGGAT
    GGCCGGACGT TTTACATTGA CCACAATAGC AAAATTACTC AGTGGGAAGA CCCAAGACTG
    CAGAACCCAG CTATTACTGG TCCGGCTGTT CCTTACTCCA GAGAATTTAA GCAGAAATAT
    GACTACTTTA GAAAGAAATT AAAGAAACCT GCGGATATTC CAAATAGGTT TGAAATGAAA
    CTTCACAGAA ATAACATATT TGAAGAGTCC TATCGGAGAA TCATGTCTGT GAAAAGACCA
    GATGTCCTGA AAGCTAGGCT GTGGATTGAG TTTGAATCGG AGAAAGGTCT GGACTACGGG
    GGCGTGGCCA GAGAGTGGTT CTTCCTACTG TCCAAGGAGA TGTTCAACCC TTACTACGGC
    CTCTTCGAGT ATTCGGCAAC GGACAACTAC ACACTTCAGA TCAATCCCAA TTCAGGCCTC
    TGTAATGAAG ATCATTTGTC CTATTTCACT TTCATTGGAA GAGTTGCTGG GCTGGCAGTA
    TTTCATGGGA AACTTCTAGA TGGTTTCTTC ATTCGACCGT TTTATAAAAT GATGCTGGGA
    AAGCAGATAA CTCTGAATGA CATGGAATCT GTGGATAGCG AATATTACAA CTCTTTGAAA
    TGGATCTTAG AAAATGACCC AACTGAACTG GACCTTATGT TCTGCATTGA TGAAGAGAAC
    TTTGGGCAGA CATATCAAGT GGATCTGAAG CCCAACGGGT CAGAAATAAT GGTAACGAAC
    GAAAACAAGA GAGAGTACAT CGACCTCGTC ATCCAGTGGA GGTTCGTGAA CAGGGTCCAG
    AAGCAGATGA ACGCCTTCCT GGAGGGATTC ACGGAACTGC TCCCTATTGA TTTGATTAAA
    ATCTTTGATG AAAATGAACT GGAGTTGCTG ATGTGTGGCC TTGGCGACGT GGATGTGAAC
    GACTGGCGAC AACATTCTAT TTACAAGAAT GGCTACTGCC CGAACCACCC CGTGATCCAG
    TGGTTCTGGA AGGCTGTGCT GCTGATGGAC GCCGAGAAGC GCATCCGGTT GCTGCAGTTT
    GTCACAGGGA CGTCCCGAGT ACCTATGAAT GGATTTGCCG AGCTTTATGG TTCCAATGGT
    CCTCAGCTGT TTACAATAGA GCAATGGGGA AGTCCTGAAA AACTGCCCAG AGCTCACACG
    TGCTTTAATC GCCTTGACTT ACCTCCGTAT GAAACCTTCG AAGATTTACG AGAGAAGCTC
    CTCATGGCTG TGGAAAACGC CCAAGGCTTC GAGGGGGTGG ATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005199810.3
    CDS554..3118
    Translation

    Target ORF information:

    RefSeq Version XM_005199753.3
    Organism Bos taurus(cattle)
    Definition Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005199753.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGCCCAGTGC CCCAGCTGGG
    AGAGCACGTT CATCAACTGT CACTGGTGGT GAGGCGCCAA CGCCATCAGT GGCCTATGTA
    CACACCACGC CGGGCCTGCC CTCGGGCTGG GAAGAGAGGA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCTATCAT GCAGCTCGCA
    GAAGACGGCG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CCGCCCCGCT GGAGGGCGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GATACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAGA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA CAACACAAAG
    ACCACGACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ACATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGCTGGG AAGAAAGGAT CCACTTGGAT
    GGCCGGACGT TTTACATTGA CCACAATAGC AAAATTACTC AGTGGGAAGA CCCAAGACTG
    CAGAACCCAG CTATTACTGG TCCGGCTGTT CCTTACTCCA GAGAATTTAA GCAGAAATAT
    GACTACTTTA GAAAGAAATT AAAGAAACCT GCGGATATTC CAAATAGGTT TGAAATGAAA
    CTTCACAGAA ATAACATATT TGAAGAGTCC TATCGGAGAA TCATGTCTGT GAAAAGACCA
    GATGTCCTGA AAGCTAGGCT GTGGATTGAG TTTGAATCGG AGAAAGGTCT GGACTACGGG
    GGCGTGGCCA GAGAGTGGTT CTTCCTACTG TCCAAGGAGA TGTTCAACCC TTACTACGGC
    CTCTTCGAGT ATTCGGCAAC GGACAACTAC ACACTTCAGA TCAATCCCAA TTCAGGCCTC
    TGTAATGAAG ATCATTTGTC CTATTTCACT TTCATTGGAA GAGTTGCTGG GCTGGCAGTA
    TTTCATGGGA AACTTCTAGA TGGTTTCTTC ATTCGACCGT TTTATAAAAT GATGCTGGGA
    AAGCAGATAA CTCTGAATGA CATGGAATCT GTGGATAGCG AATATTACAA CTCTTTGAAA
    TGGATCTTAG AAAATGACCC AACTGAACTG GACCTTATGT TCTGCATTGA TGAAGAGAAC
    TTTGGGCAGA CATATCAAGT GGATCTGAAG CCCAACGGGT CAGAAATAAT GGTAACGAAC
    GAAAACAAGA GAGAGTACAT CGACCTCGTC ATCCAGTGGA GGTTCGTGAA CAGGGTCCAG
    AAGCAGATGA ACGCCTTCCT GGAGGGATTC ACGGAACTGC TCCCTATTGA TTTGATTAAA
    ATCTTTGATG AAAATGAACT GGAGTTGCTG ATGTGTGGCC TTGGCGACGT GGATGTGAAC
    GACTGGCGAC AACATTCTAT TTACAAGAAT GGCTACTGCC CGAACCACCC CGTGATCCAG
    TGGTTCTGGA AGGCTGTGCT GCTGATGGAC GCCGAGAAGC GCATCCGGTT GCTGCAGTTT
    GTCACAGGGA CGTCCCGAGT ACCTATGAAT GGATTTGCCG AGCTTTATGG TTCCAATGGT
    CCTCAGCTGT TTACAATAGA GCAATGGGGA AGTCCTGAAA AACTGCCCAG AGCTCACACG
    TGCTTTAATC GCCTTGACTT ACCTCCGTAT GAAACCTTCG AAGATTTACG AGAGAAGCTC
    CTCATGGCTG TGGAAAACGC CCAAGGCTTC GAGGGGGTGG ATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88365
    Clone ID Related Accession (Same CDS sequence) XM_002697776.5 , XM_002697776.6 , XM_005199753.3 , XM_015469187.1 , XM_015460158.1 , XM_024984480.1 , XM_024984481.1
    Accession Version XM_015469187.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2565bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646636.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGCCCAGTGC CCCAGCTGGG
    AGAGCACGTT CATCAACTGT CACTGGTGGT GAGGCGCCAA CGCCATCAGT GGCCTATGTA
    CACACCACGC CGGGCCTGCC CTCGGGCTGG GAAGAGAGGA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCTATCAT GCAGCTCGCA
    GAAGACGGCG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CCGCCCCGCT GGAGGGCGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GATACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAGA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA CAACACAAAG
    ACCACGACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ACATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGCTGGG AAGAAAGGAT CCACTTGGAT
    GGCCGGACGT TTTACATTGA CCACAATAGC AAAATTACTC AGTGGGAAGA CCCAAGACTG
    CAGAACCCAG CTATTACTGG TCCGGCTGTT CCTTACTCCA GAGAATTTAA GCAGAAATAT
    GACTACTTTA GAAAGAAATT AAAGAAACCT GCGGATATTC CAAATAGGTT TGAAATGAAA
    CTTCACAGAA ATAACATATT TGAAGAGTCC TATCGGAGAA TCATGTCTGT GAAAAGACCA
    GATGTCCTGA AAGCTAGGCT GTGGATTGAG TTTGAATCGG AGAAAGGTCT GGACTACGGG
    GGCGTGGCCA GAGAGTGGTT CTTCCTACTG TCCAAGGAGA TGTTCAACCC TTACTACGGC
    CTCTTCGAGT ATTCGGCAAC GGACAACTAC ACACTTCAGA TCAATCCCAA TTCAGGCCTC
    TGTAATGAAG ATCATTTGTC CTATTTCACT TTCATTGGAA GAGTTGCTGG GCTGGCAGTA
    TTTCATGGGA AACTTCTAGA TGGTTTCTTC ATTCGACCGT TTTATAAAAT GATGCTGGGA
    AAGCAGATAA CTCTGAATGA CATGGAATCT GTGGATAGCG AATATTACAA CTCTTTGAAA
    TGGATCTTAG AAAATGACCC AACTGAACTG GACCTTATGT TCTGCATTGA TGAAGAGAAC
    TTTGGGCAGA CATATCAAGT GGATCTGAAG CCCAACGGGT CAGAAATAAT GGTAACGAAC
    GAAAACAAGA GAGAGTACAT CGACCTCGTC ATCCAGTGGA GGTTCGTGAA CAGGGTCCAG
    AAGCAGATGA ACGCCTTCCT GGAGGGATTC ACGGAACTGC TCCCTATTGA TTTGATTAAA
    ATCTTTGATG AAAATGAACT GGAGTTGCTG ATGTGTGGCC TTGGCGACGT GGATGTGAAC
    GACTGGCGAC AACATTCTAT TTACAAGAAT GGCTACTGCC CGAACCACCC CGTGATCCAG
    TGGTTCTGGA AGGCTGTGCT GCTGATGGAC GCCGAGAAGC GCATCCGGTT GCTGCAGTTT
    GTCACAGGGA CGTCCCGAGT ACCTATGAAT GGATTTGCCG AGCTTTATGG TTCCAATGGT
    CCTCAGCTGT TTACAATAGA GCAATGGGGA AGTCCTGAAA AACTGCCCAG AGCTCACACG
    TGCTTTAATC GCCTTGACTT ACCTCCGTAT GAAACCTTCG AAGATTTACG AGAGAAGCTC
    CTCATGGCTG TGGAAAACGC CCAAGGCTTC GAGGGGGTGG ATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015324673.1
    CDS316..2880
    Translation

    Target ORF information:

    RefSeq Version XM_015469187.1
    Organism Bos taurus(cattle)
    Definition Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015469187.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGCCCAGTGC CCCAGCTGGG
    AGAGCACGTT CATCAACTGT CACTGGTGGT GAGGCGCCAA CGCCATCAGT GGCCTATGTA
    CACACCACGC CGGGCCTGCC CTCGGGCTGG GAAGAGAGGA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCTATCAT GCAGCTCGCA
    GAAGACGGCG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CCGCCCCGCT GGAGGGCGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GATACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAGA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA CAACACAAAG
    ACCACGACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ACATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGCTGGG AAGAAAGGAT CCACTTGGAT
    GGCCGGACGT TTTACATTGA CCACAATAGC AAAATTACTC AGTGGGAAGA CCCAAGACTG
    CAGAACCCAG CTATTACTGG TCCGGCTGTT CCTTACTCCA GAGAATTTAA GCAGAAATAT
    GACTACTTTA GAAAGAAATT AAAGAAACCT GCGGATATTC CAAATAGGTT TGAAATGAAA
    CTTCACAGAA ATAACATATT TGAAGAGTCC TATCGGAGAA TCATGTCTGT GAAAAGACCA
    GATGTCCTGA AAGCTAGGCT GTGGATTGAG TTTGAATCGG AGAAAGGTCT GGACTACGGG
    GGCGTGGCCA GAGAGTGGTT CTTCCTACTG TCCAAGGAGA TGTTCAACCC TTACTACGGC
    CTCTTCGAGT ATTCGGCAAC GGACAACTAC ACACTTCAGA TCAATCCCAA TTCAGGCCTC
    TGTAATGAAG ATCATTTGTC CTATTTCACT TTCATTGGAA GAGTTGCTGG GCTGGCAGTA
    TTTCATGGGA AACTTCTAGA TGGTTTCTTC ATTCGACCGT TTTATAAAAT GATGCTGGGA
    AAGCAGATAA CTCTGAATGA CATGGAATCT GTGGATAGCG AATATTACAA CTCTTTGAAA
    TGGATCTTAG AAAATGACCC AACTGAACTG GACCTTATGT TCTGCATTGA TGAAGAGAAC
    TTTGGGCAGA CATATCAAGT GGATCTGAAG CCCAACGGGT CAGAAATAAT GGTAACGAAC
    GAAAACAAGA GAGAGTACAT CGACCTCGTC ATCCAGTGGA GGTTCGTGAA CAGGGTCCAG
    AAGCAGATGA ACGCCTTCCT GGAGGGATTC ACGGAACTGC TCCCTATTGA TTTGATTAAA
    ATCTTTGATG AAAATGAACT GGAGTTGCTG ATGTGTGGCC TTGGCGACGT GGATGTGAAC
    GACTGGCGAC AACATTCTAT TTACAAGAAT GGCTACTGCC CGAACCACCC CGTGATCCAG
    TGGTTCTGGA AGGCTGTGCT GCTGATGGAC GCCGAGAAGC GCATCCGGTT GCTGCAGTTT
    GTCACAGGGA CGTCCCGAGT ACCTATGAAT GGATTTGCCG AGCTTTATGG TTCCAATGGT
    CCTCAGCTGT TTACAATAGA GCAATGGGGA AGTCCTGAAA AACTGCCCAG AGCTCACACG
    TGCTTTAATC GCCTTGACTT ACCTCCGTAT GAAACCTTCG AAGATTTACG AGAGAAGCTC
    CTCATGGCTG TGGAAAACGC CCAAGGCTTC GAGGGGGTGG ATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88365
    Clone ID Related Accession (Same CDS sequence) XM_002697776.5 , XM_002697776.6 , XM_005199753.3 , XM_015469187.1 , XM_015460158.1 , XM_024984480.1 , XM_024984481.1
    Accession Version XM_015460158.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2565bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104569.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGCCCAGTGC CCCAGCTGGG
    AGAGCACGTT CATCAACTGT CACTGGTGGT GAGGCGCCAA CGCCATCAGT GGCCTATGTA
    CACACCACGC CGGGCCTGCC CTCGGGCTGG GAAGAGAGGA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCTATCAT GCAGCTCGCA
    GAAGACGGCG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CCGCCCCGCT GGAGGGCGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GATACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAGA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA CAACACAAAG
    ACCACGACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ACATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGCTGGG AAGAAAGGAT CCACTTGGAT
    GGCCGGACGT TTTACATTGA CCACAATAGC AAAATTACTC AGTGGGAAGA CCCAAGACTG
    CAGAACCCAG CTATTACTGG TCCGGCTGTT CCTTACTCCA GAGAATTTAA GCAGAAATAT
    GACTACTTTA GAAAGAAATT AAAGAAACCT GCGGATATTC CAAATAGGTT TGAAATGAAA
    CTTCACAGAA ATAACATATT TGAAGAGTCC TATCGGAGAA TCATGTCTGT GAAAAGACCA
    GATGTCCTGA AAGCTAGGCT GTGGATTGAG TTTGAATCGG AGAAAGGTCT GGACTACGGG
    GGCGTGGCCA GAGAGTGGTT CTTCCTACTG TCCAAGGAGA TGTTCAACCC TTACTACGGC
    CTCTTCGAGT ATTCGGCAAC GGACAACTAC ACACTTCAGA TCAATCCCAA TTCAGGCCTC
    TGTAATGAAG ATCATTTGTC CTATTTCACT TTCATTGGAA GAGTTGCTGG GCTGGCAGTA
    TTTCATGGGA AACTTCTAGA TGGTTTCTTC ATTCGACCGT TTTATAAAAT GATGCTGGGA
    AAGCAGATAA CTCTGAATGA CATGGAATCT GTGGATAGCG AATATTACAA CTCTTTGAAA
    TGGATCTTAG AAAATGACCC AACTGAACTG GACCTTATGT TCTGCATTGA TGAAGAGAAC
    TTTGGGCAGA CATATCAAGT GGATCTGAAG CCCAACGGGT CAGAAATAAT GGTAACGAAC
    GAAAACAAGA GAGAGTACAT CGACCTCGTC ATCCAGTGGA GGTTCGTGAA CAGGGTCCAG
    AAGCAGATGA ACGCCTTCCT GGAGGGATTC ACGGAACTGC TCCCTATTGA TTTGATTAAA
    ATCTTTGATG AAAATGAACT GGAGTTGCTG ATGTGTGGCC TTGGCGACGT GGATGTGAAC
    GACTGGCGAC AACATTCTAT TTACAAGAAT GGCTACTGCC CGAACCACCC CGTGATCCAG
    TGGTTCTGGA AGGCTGTGCT GCTGATGGAC GCCGAGAAGC GCATCCGGTT GCTGCAGTTT
    GTCACAGGGA CGTCCCGAGT ACCTATGAAT GGATTTGCCG AGCTTTATGG TTCCAATGGT
    CCTCAGCTGT TTACAATAGA GCAATGGGGA AGTCCTGAAA AACTGCCCAG AGCTCACACG
    TGCTTTAATC GCCTTGACTT ACCTCCGTAT GAAACCTTCG AAGATTTACG AGAGAAGCTC
    CTCATGGCTG TGGAAAACGC CCAAGGCTTC GAGGGGGTGG ATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015315644.1
    CDS316..2880
    Translation

    Target ORF information:

    RefSeq Version XM_015460158.1
    Organism Bos taurus(cattle)
    Definition Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015460158.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGCCCAGTGC CCCAGCTGGG
    AGAGCACGTT CATCAACTGT CACTGGTGGT GAGGCGCCAA CGCCATCAGT GGCCTATGTA
    CACACCACGC CGGGCCTGCC CTCGGGCTGG GAAGAGAGGA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCTATCAT GCAGCTCGCA
    GAAGACGGCG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CCGCCCCGCT GGAGGGCGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GATACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAGA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA CAACACAAAG
    ACCACGACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ACATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGCTGGG AAGAAAGGAT CCACTTGGAT
    GGCCGGACGT TTTACATTGA CCACAATAGC AAAATTACTC AGTGGGAAGA CCCAAGACTG
    CAGAACCCAG CTATTACTGG TCCGGCTGTT CCTTACTCCA GAGAATTTAA GCAGAAATAT
    GACTACTTTA GAAAGAAATT AAAGAAACCT GCGGATATTC CAAATAGGTT TGAAATGAAA
    CTTCACAGAA ATAACATATT TGAAGAGTCC TATCGGAGAA TCATGTCTGT GAAAAGACCA
    GATGTCCTGA AAGCTAGGCT GTGGATTGAG TTTGAATCGG AGAAAGGTCT GGACTACGGG
    GGCGTGGCCA GAGAGTGGTT CTTCCTACTG TCCAAGGAGA TGTTCAACCC TTACTACGGC
    CTCTTCGAGT ATTCGGCAAC GGACAACTAC ACACTTCAGA TCAATCCCAA TTCAGGCCTC
    TGTAATGAAG ATCATTTGTC CTATTTCACT TTCATTGGAA GAGTTGCTGG GCTGGCAGTA
    TTTCATGGGA AACTTCTAGA TGGTTTCTTC ATTCGACCGT TTTATAAAAT GATGCTGGGA
    AAGCAGATAA CTCTGAATGA CATGGAATCT GTGGATAGCG AATATTACAA CTCTTTGAAA
    TGGATCTTAG AAAATGACCC AACTGAACTG GACCTTATGT TCTGCATTGA TGAAGAGAAC
    TTTGGGCAGA CATATCAAGT GGATCTGAAG CCCAACGGGT CAGAAATAAT GGTAACGAAC
    GAAAACAAGA GAGAGTACAT CGACCTCGTC ATCCAGTGGA GGTTCGTGAA CAGGGTCCAG
    AAGCAGATGA ACGCCTTCCT GGAGGGATTC ACGGAACTGC TCCCTATTGA TTTGATTAAA
    ATCTTTGATG AAAATGAACT GGAGTTGCTG ATGTGTGGCC TTGGCGACGT GGATGTGAAC
    GACTGGCGAC AACATTCTAT TTACAAGAAT GGCTACTGCC CGAACCACCC CGTGATCCAG
    TGGTTCTGGA AGGCTGTGCT GCTGATGGAC GCCGAGAAGC GCATCCGGTT GCTGCAGTTT
    GTCACAGGGA CGTCCCGAGT ACCTATGAAT GGATTTGCCG AGCTTTATGG TTCCAATGGT
    CCTCAGCTGT TTACAATAGA GCAATGGGGA AGTCCTGAAA AACTGCCCAG AGCTCACACG
    TGCTTTAATC GCCTTGACTT ACCTCCGTAT GAAACCTTCG AAGATTTACG AGAGAAGCTC
    CTCATGGCTG TGGAAAACGC CCAAGGCTTC GAGGGGGTGG ATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88365
    Clone ID Related Accession (Same CDS sequence) XM_002697776.5 , XM_002697776.6 , XM_005199753.3 , XM_015469187.1 , XM_015460158.1 , XM_024984480.1 , XM_024984481.1
    Accession Version XM_024984480.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2565bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGCCCAGTGC CCCAGCTGGG
    AGAGCACGTT CATCAACTGT CACTGGTGGT GAGGCGCCAA CGCCATCAGT GGCCTATGTA
    CACACCACGC CGGGCCTGCC CTCGGGCTGG GAAGAGAGGA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCTATCAT GCAGCTCGCA
    GAAGACGGCG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CCGCCCCGCT GGAGGGCGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GATACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAGA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA CAACACAAAG
    ACCACGACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ACATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGCTGGG AAGAAAGGAT CCACTTGGAT
    GGCCGGACGT TTTACATTGA CCACAATAGC AAAATTACTC AGTGGGAAGA CCCAAGACTG
    CAGAACCCAG CTATTACTGG TCCGGCTGTT CCTTACTCCA GAGAATTTAA GCAGAAATAT
    GACTACTTTA GAAAGAAATT AAAGAAACCT GCGGATATTC CAAATAGGTT TGAAATGAAA
    CTTCACAGAA ATAACATATT TGAAGAGTCC TATCGGAGAA TCATGTCTGT GAAAAGACCA
    GATGTCCTGA AAGCTAGGCT GTGGATTGAG TTTGAATCGG AGAAAGGTCT GGACTACGGG
    GGCGTGGCCA GAGAGTGGTT CTTCCTACTG TCCAAGGAGA TGTTCAACCC TTACTACGGC
    CTCTTCGAGT ATTCGGCAAC GGACAACTAC ACACTTCAGA TCAATCCCAA TTCAGGCCTC
    TGTAATGAAG ATCATTTGTC CTATTTCACT TTCATTGGAA GAGTTGCTGG GCTGGCAGTA
    TTTCATGGGA AACTTCTAGA TGGTTTCTTC ATTCGACCGT TTTATAAAAT GATGCTGGGA
    AAGCAGATAA CTCTGAATGA CATGGAATCT GTGGATAGCG AATATTACAA CTCTTTGAAA
    TGGATCTTAG AAAATGACCC AACTGAACTG GACCTTATGT TCTGCATTGA TGAAGAGAAC
    TTTGGGCAGA CATATCAAGT GGATCTGAAG CCCAACGGGT CAGAAATAAT GGTAACGAAC
    GAAAACAAGA GAGAGTACAT CGACCTCGTC ATCCAGTGGA GGTTCGTGAA CAGGGTCCAG
    AAGCAGATGA ACGCCTTCCT GGAGGGATTC ACGGAACTGC TCCCTATTGA TTTGATTAAA
    ATCTTTGATG AAAATGAACT GGAGTTGCTG ATGTGTGGCC TTGGCGACGT GGATGTGAAC
    GACTGGCGAC AACATTCTAT TTACAAGAAT GGCTACTGCC CGAACCACCC CGTGATCCAG
    TGGTTCTGGA AGGCTGTGCT GCTGATGGAC GCCGAGAAGC GCATCCGGTT GCTGCAGTTT
    GTCACAGGGA CGTCCCGAGT ACCTATGAAT GGATTTGCCG AGCTTTATGG TTCCAATGGT
    CCTCAGCTGT TTACAATAGA GCAATGGGGA AGTCCTGAAA AACTGCCCAG AGCTCACACG
    TGCTTTAATC GCCTTGACTT ACCTCCGTAT GAAACCTTCG AAGATTTACG AGAGAAGCTC
    CTCATGGCTG TGGAAAACGC CCAAGGCTTC GAGGGGGTGG ATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024840248.1
    CDS390..2954
    Translation

    Target ORF information:

    RefSeq Version XM_024984480.1
    Organism Bos taurus(cattle)
    Definition Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X20, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024984480.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGCCCAGTGC CCCAGCTGGG
    AGAGCACGTT CATCAACTGT CACTGGTGGT GAGGCGCCAA CGCCATCAGT GGCCTATGTA
    CACACCACGC CGGGCCTGCC CTCGGGCTGG GAAGAGAGGA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCTATCAT GCAGCTCGCA
    GAAGACGGCG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CCGCCCCGCT GGAGGGCGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GATACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAGA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA CAACACAAAG
    ACCACGACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ACATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGCTGGG AAGAAAGGAT CCACTTGGAT
    GGCCGGACGT TTTACATTGA CCACAATAGC AAAATTACTC AGTGGGAAGA CCCAAGACTG
    CAGAACCCAG CTATTACTGG TCCGGCTGTT CCTTACTCCA GAGAATTTAA GCAGAAATAT
    GACTACTTTA GAAAGAAATT AAAGAAACCT GCGGATATTC CAAATAGGTT TGAAATGAAA
    CTTCACAGAA ATAACATATT TGAAGAGTCC TATCGGAGAA TCATGTCTGT GAAAAGACCA
    GATGTCCTGA AAGCTAGGCT GTGGATTGAG TTTGAATCGG AGAAAGGTCT GGACTACGGG
    GGCGTGGCCA GAGAGTGGTT CTTCCTACTG TCCAAGGAGA TGTTCAACCC TTACTACGGC
    CTCTTCGAGT ATTCGGCAAC GGACAACTAC ACACTTCAGA TCAATCCCAA TTCAGGCCTC
    TGTAATGAAG ATCATTTGTC CTATTTCACT TTCATTGGAA GAGTTGCTGG GCTGGCAGTA
    TTTCATGGGA AACTTCTAGA TGGTTTCTTC ATTCGACCGT TTTATAAAAT GATGCTGGGA
    AAGCAGATAA CTCTGAATGA CATGGAATCT GTGGATAGCG AATATTACAA CTCTTTGAAA
    TGGATCTTAG AAAATGACCC AACTGAACTG GACCTTATGT TCTGCATTGA TGAAGAGAAC
    TTTGGGCAGA CATATCAAGT GGATCTGAAG CCCAACGGGT CAGAAATAAT GGTAACGAAC
    GAAAACAAGA GAGAGTACAT CGACCTCGTC ATCCAGTGGA GGTTCGTGAA CAGGGTCCAG
    AAGCAGATGA ACGCCTTCCT GGAGGGATTC ACGGAACTGC TCCCTATTGA TTTGATTAAA
    ATCTTTGATG AAAATGAACT GGAGTTGCTG ATGTGTGGCC TTGGCGACGT GGATGTGAAC
    GACTGGCGAC AACATTCTAT TTACAAGAAT GGCTACTGCC CGAACCACCC CGTGATCCAG
    TGGTTCTGGA AGGCTGTGCT GCTGATGGAC GCCGAGAAGC GCATCCGGTT GCTGCAGTTT
    GTCACAGGGA CGTCCCGAGT ACCTATGAAT GGATTTGCCG AGCTTTATGG TTCCAATGGT
    CCTCAGCTGT TTACAATAGA GCAATGGGGA AGTCCTGAAA AACTGCCCAG AGCTCACACG
    TGCTTTAATC GCCTTGACTT ACCTCCGTAT GAAACCTTCG AAGATTTACG AGAGAAGCTC
    CTCATGGCTG TGGAAAACGC CCAAGGCTTC GAGGGGGTGG ATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88365
    Clone ID Related Accession (Same CDS sequence) XM_002697776.5 , XM_002697776.6 , XM_005199753.3 , XM_015469187.1 , XM_015460158.1 , XM_024984480.1 , XM_024984481.1
    Accession Version XM_024984481.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2565bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGCCCAGTGC CCCAGCTGGG
    AGAGCACGTT CATCAACTGT CACTGGTGGT GAGGCGCCAA CGCCATCAGT GGCCTATGTA
    CACACCACGC CGGGCCTGCC CTCGGGCTGG GAAGAGAGGA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCTATCAT GCAGCTCGCA
    GAAGACGGCG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CCGCCCCGCT GGAGGGCGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GATACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAGA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA CAACACAAAG
    ACCACGACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ACATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGCTGGG AAGAAAGGAT CCACTTGGAT
    GGCCGGACGT TTTACATTGA CCACAATAGC AAAATTACTC AGTGGGAAGA CCCAAGACTG
    CAGAACCCAG CTATTACTGG TCCGGCTGTT CCTTACTCCA GAGAATTTAA GCAGAAATAT
    GACTACTTTA GAAAGAAATT AAAGAAACCT GCGGATATTC CAAATAGGTT TGAAATGAAA
    CTTCACAGAA ATAACATATT TGAAGAGTCC TATCGGAGAA TCATGTCTGT GAAAAGACCA
    GATGTCCTGA AAGCTAGGCT GTGGATTGAG TTTGAATCGG AGAAAGGTCT GGACTACGGG
    GGCGTGGCCA GAGAGTGGTT CTTCCTACTG TCCAAGGAGA TGTTCAACCC TTACTACGGC
    CTCTTCGAGT ATTCGGCAAC GGACAACTAC ACACTTCAGA TCAATCCCAA TTCAGGCCTC
    TGTAATGAAG ATCATTTGTC CTATTTCACT TTCATTGGAA GAGTTGCTGG GCTGGCAGTA
    TTTCATGGGA AACTTCTAGA TGGTTTCTTC ATTCGACCGT TTTATAAAAT GATGCTGGGA
    AAGCAGATAA CTCTGAATGA CATGGAATCT GTGGATAGCG AATATTACAA CTCTTTGAAA
    TGGATCTTAG AAAATGACCC AACTGAACTG GACCTTATGT TCTGCATTGA TGAAGAGAAC
    TTTGGGCAGA CATATCAAGT GGATCTGAAG CCCAACGGGT CAGAAATAAT GGTAACGAAC
    GAAAACAAGA GAGAGTACAT CGACCTCGTC ATCCAGTGGA GGTTCGTGAA CAGGGTCCAG
    AAGCAGATGA ACGCCTTCCT GGAGGGATTC ACGGAACTGC TCCCTATTGA TTTGATTAAA
    ATCTTTGATG AAAATGAACT GGAGTTGCTG ATGTGTGGCC TTGGCGACGT GGATGTGAAC
    GACTGGCGAC AACATTCTAT TTACAAGAAT GGCTACTGCC CGAACCACCC CGTGATCCAG
    TGGTTCTGGA AGGCTGTGCT GCTGATGGAC GCCGAGAAGC GCATCCGGTT GCTGCAGTTT
    GTCACAGGGA CGTCCCGAGT ACCTATGAAT GGATTTGCCG AGCTTTATGG TTCCAATGGT
    CCTCAGCTGT TTACAATAGA GCAATGGGGA AGTCCTGAAA AACTGCCCAG AGCTCACACG
    TGCTTTAATC GCCTTGACTT ACCTCCGTAT GAAACCTTCG AAGATTTACG AGAGAAGCTC
    CTCATGGCTG TGGAAAACGC CCAAGGCTTC GAGGGGGTGG ATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024840249.1
    CDS458..3022
    Translation

    Target ORF information:

    RefSeq Version XM_024984481.1
    Organism Bos taurus(cattle)
    Definition Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X21, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024984481.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGCCCAGTGC CCCAGCTGGG
    AGAGCACGTT CATCAACTGT CACTGGTGGT GAGGCGCCAA CGCCATCAGT GGCCTATGTA
    CACACCACGC CGGGCCTGCC CTCGGGCTGG GAAGAGAGGA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCTATCAT GCAGCTCGCA
    GAAGACGGCG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CCGCCCCGCT GGAGGGCGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GATACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAGA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA CAACACAAAG
    ACCACGACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ACATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGCTGGG AAGAAAGGAT CCACTTGGAT
    GGCCGGACGT TTTACATTGA CCACAATAGC AAAATTACTC AGTGGGAAGA CCCAAGACTG
    CAGAACCCAG CTATTACTGG TCCGGCTGTT CCTTACTCCA GAGAATTTAA GCAGAAATAT
    GACTACTTTA GAAAGAAATT AAAGAAACCT GCGGATATTC CAAATAGGTT TGAAATGAAA
    CTTCACAGAA ATAACATATT TGAAGAGTCC TATCGGAGAA TCATGTCTGT GAAAAGACCA
    GATGTCCTGA AAGCTAGGCT GTGGATTGAG TTTGAATCGG AGAAAGGTCT GGACTACGGG
    GGCGTGGCCA GAGAGTGGTT CTTCCTACTG TCCAAGGAGA TGTTCAACCC TTACTACGGC
    CTCTTCGAGT ATTCGGCAAC GGACAACTAC ACACTTCAGA TCAATCCCAA TTCAGGCCTC
    TGTAATGAAG ATCATTTGTC CTATTTCACT TTCATTGGAA GAGTTGCTGG GCTGGCAGTA
    TTTCATGGGA AACTTCTAGA TGGTTTCTTC ATTCGACCGT TTTATAAAAT GATGCTGGGA
    AAGCAGATAA CTCTGAATGA CATGGAATCT GTGGATAGCG AATATTACAA CTCTTTGAAA
    TGGATCTTAG AAAATGACCC AACTGAACTG GACCTTATGT TCTGCATTGA TGAAGAGAAC
    TTTGGGCAGA CATATCAAGT GGATCTGAAG CCCAACGGGT CAGAAATAAT GGTAACGAAC
    GAAAACAAGA GAGAGTACAT CGACCTCGTC ATCCAGTGGA GGTTCGTGAA CAGGGTCCAG
    AAGCAGATGA ACGCCTTCCT GGAGGGATTC ACGGAACTGC TCCCTATTGA TTTGATTAAA
    ATCTTTGATG AAAATGAACT GGAGTTGCTG ATGTGTGGCC TTGGCGACGT GGATGTGAAC
    GACTGGCGAC AACATTCTAT TTACAAGAAT GGCTACTGCC CGAACCACCC CGTGATCCAG
    TGGTTCTGGA AGGCTGTGCT GCTGATGGAC GCCGAGAAGC GCATCCGGTT GCTGCAGTTT
    GTCACAGGGA CGTCCCGAGT ACCTATGAAT GGATTTGCCG AGCTTTATGG TTCCAATGGT
    CCTCAGCTGT TTACAATAGA GCAATGGGGA AGTCCTGAAA AACTGCCCAG AGCTCACACG
    TGCTTTAATC GCCTTGACTT ACCTCCGTAT GAAACCTTCG AAGATTTACG AGAGAAGCTC
    CTCATGGCTG TGGAAAACGC CCAAGGCTTC GAGGGGGTGG ATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88366
    Clone ID Related Accession (Same CDS sequence) XM_024984482.1 , XM_005199754.3 , XM_005224352.4 , XM_005224352.3
    Accession Version XM_024984482.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2559bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGCCATCAGT GGCCTATGTA
    CACACCACGC CGGGCCTGCC CTCGGGCTGG GAAGAGAGGA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCTATCAT GCAGCTCGCA
    GAAGACGGCG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CCGCCCCGCT GGAGGGCGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GATACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAGA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA CAACACAAAG
    ACCACGACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ACATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGCTGGG AAGAAAGGAT CCACTTGGAT
    GGCCGGACGT TTTACATTGA CCACAGCAGC CAGGTGTTAT GTGGCGAGGA CGATGCCAGA
    GACTCAGTGC CCTCGTACGA TAGCAAAATT ACTCAGTGGG AAGACCCAAG ACTGCAGAAC
    CCAGCTATTA CTGGTCCGGC TGTTCCTTAC TCCAGAGAAT TTAAGCAGAA ATATGACTAC
    TTTAGAAAGA AATTAAAGAA ACCTGCGGAT ATTCCAAATA GGTTTGAAAT GAAACTTCAC
    AGAAATAACA TATTTGAAGA GTCCTATCGG AGAATCATGT CTGTGAAAAG ACCAGATGTC
    CTGAAAGCTA GGCTGTGGAT TGAGTTTGAA TCGGAGAAAG GTCTGGACTA CGGGGGCGTG
    GCCAGAGAGT GGTTCTTCCT ACTGTCCAAG GAGATGTTCA ACCCTTACTA CGGCCTCTTC
    GAGTATTCGG CAACGGACAA CTACACACTT CAGATCAATC CCAATTCAGG CCTCTGTAAT
    GAAGATCATT TGTCCTATTT CACTTTCATT GGAAGAGTTG CTGGGCTGGC AGTATTTCAT
    GGGAAACTTC TAGATGGTTT CTTCATTCGA CCGTTTTATA AAATGATGCT GGGAAAGCAG
    ATAACTCTGA ATGACATGGA ATCTGTGGAT AGCGAATATT ACAACTCTTT GAAATGGATC
    TTAGAAAATG ACCCAACTGA ACTGGACCTT ATGTTCTGCA TTGATGAAGA GAACTTTGGG
    CAGACATATC AAGTGGATCT GAAGCCCAAC GGGTCAGAAA TAATGGTAAC GAACGAAAAC
    AAGAGAGAGT ACATCGACCT CGTCATCCAG TGGAGGTTCG TGAACAGGGT CCAGAAGCAG
    ATGAACGCCT TCCTGGAGGG ATTCACGGAA CTGCTCCCTA TTGATTTGAT TAAAATCTTT
    GATGAAAATG AACTGGAGTT GCTGATGTGT GGCCTTGGCG ACGTGGATGT GAACGACTGG
    CGACAACATT CTATTTACAA GAATGGCTAC TGCCCGAACC ACCCCGTGAT CCAGTGGTTC
    TGGAAGGCTG TGCTGCTGAT GGACGCCGAG AAGCGCATCC GGTTGCTGCA GTTTGTCACA
    GGGACGTCCC GAGTACCTAT GAATGGATTT GCCGAGCTTT ATGGTTCCAA TGGTCCTCAG
    CTGTTTACAA TAGAGCAATG GGGAAGTCCT GAAAAACTGC CCAGAGCTCA CACGTGCTTT
    AATCGCCTTG ACTTACCTCC GTATGAAACC TTCGAAGATT TACGAGAGAA GCTCCTCATG
    GCTGTGGAAA ACGCCCAAGG CTTCGAGGGG GTGGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024840250.1
    CDS267..2825
    Translation

    Target ORF information:

    RefSeq Version XM_024984482.1
    Organism Bos taurus(cattle)
    Definition Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X22, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024984482.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGCCATCAGT GGCCTATGTA
    CACACCACGC CGGGCCTGCC CTCGGGCTGG GAAGAGAGGA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCTATCAT GCAGCTCGCA
    GAAGACGGCG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CCGCCCCGCT GGAGGGCGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GATACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAGA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA CAACACAAAG
    ACCACGACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ACATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGCTGGG AAGAAAGGAT CCACTTGGAT
    GGCCGGACGT TTTACATTGA CCACAGCAGC CAGGTGTTAT GTGGCGAGGA CGATGCCAGA
    GACTCAGTGC CCTCGTACGA TAGCAAAATT ACTCAGTGGG AAGACCCAAG ACTGCAGAAC
    CCAGCTATTA CTGGTCCGGC TGTTCCTTAC TCCAGAGAAT TTAAGCAGAA ATATGACTAC
    TTTAGAAAGA AATTAAAGAA ACCTGCGGAT ATTCCAAATA GGTTTGAAAT GAAACTTCAC
    AGAAATAACA TATTTGAAGA GTCCTATCGG AGAATCATGT CTGTGAAAAG ACCAGATGTC
    CTGAAAGCTA GGCTGTGGAT TGAGTTTGAA TCGGAGAAAG GTCTGGACTA CGGGGGCGTG
    GCCAGAGAGT GGTTCTTCCT ACTGTCCAAG GAGATGTTCA ACCCTTACTA CGGCCTCTTC
    GAGTATTCGG CAACGGACAA CTACACACTT CAGATCAATC CCAATTCAGG CCTCTGTAAT
    GAAGATCATT TGTCCTATTT CACTTTCATT GGAAGAGTTG CTGGGCTGGC AGTATTTCAT
    GGGAAACTTC TAGATGGTTT CTTCATTCGA CCGTTTTATA AAATGATGCT GGGAAAGCAG
    ATAACTCTGA ATGACATGGA ATCTGTGGAT AGCGAATATT ACAACTCTTT GAAATGGATC
    TTAGAAAATG ACCCAACTGA ACTGGACCTT ATGTTCTGCA TTGATGAAGA GAACTTTGGG
    CAGACATATC AAGTGGATCT GAAGCCCAAC GGGTCAGAAA TAATGGTAAC GAACGAAAAC
    AAGAGAGAGT ACATCGACCT CGTCATCCAG TGGAGGTTCG TGAACAGGGT CCAGAAGCAG
    ATGAACGCCT TCCTGGAGGG ATTCACGGAA CTGCTCCCTA TTGATTTGAT TAAAATCTTT
    GATGAAAATG AACTGGAGTT GCTGATGTGT GGCCTTGGCG ACGTGGATGT GAACGACTGG
    CGACAACATT CTATTTACAA GAATGGCTAC TGCCCGAACC ACCCCGTGAT CCAGTGGTTC
    TGGAAGGCTG TGCTGCTGAT GGACGCCGAG AAGCGCATCC GGTTGCTGCA GTTTGTCACA
    GGGACGTCCC GAGTACCTAT GAATGGATTT GCCGAGCTTT ATGGTTCCAA TGGTCCTCAG
    CTGTTTACAA TAGAGCAATG GGGAAGTCCT GAAAAACTGC CCAGAGCTCA CACGTGCTTT
    AATCGCCTTG ACTTACCTCC GTATGAAACC TTCGAAGATT TACGAGAGAA GCTCCTCATG
    GCTGTGGAAA ACGCCCAAGG CTTCGAGGGG GTGGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88366
    Clone ID Related Accession (Same CDS sequence) XM_024984482.1 , XM_005199754.3 , XM_005224352.4 , XM_005224352.3
    Accession Version XM_005199754.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2559bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646636.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005199754.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGCCATCAGT GGCCTATGTA
    CACACCACGC CGGGCCTGCC CTCGGGCTGG GAAGAGAGGA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCTATCAT GCAGCTCGCA
    GAAGACGGCG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CCGCCCCGCT GGAGGGCGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GATACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAGA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA CAACACAAAG
    ACCACGACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ACATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGCTGGG AAGAAAGGAT CCACTTGGAT
    GGCCGGACGT TTTACATTGA CCACAGCAGC CAGGTGTTAT GTGGCGAGGA CGATGCCAGA
    GACTCAGTGC CCTCGTACGA TAGCAAAATT ACTCAGTGGG AAGACCCAAG ACTGCAGAAC
    CCAGCTATTA CTGGTCCGGC TGTTCCTTAC TCCAGAGAAT TTAAGCAGAA ATATGACTAC
    TTTAGAAAGA AATTAAAGAA ACCTGCGGAT ATTCCAAATA GGTTTGAAAT GAAACTTCAC
    AGAAATAACA TATTTGAAGA GTCCTATCGG AGAATCATGT CTGTGAAAAG ACCAGATGTC
    CTGAAAGCTA GGCTGTGGAT TGAGTTTGAA TCGGAGAAAG GTCTGGACTA CGGGGGCGTG
    GCCAGAGAGT GGTTCTTCCT ACTGTCCAAG GAGATGTTCA ACCCTTACTA CGGCCTCTTC
    GAGTATTCGG CAACGGACAA CTACACACTT CAGATCAATC CCAATTCAGG CCTCTGTAAT
    GAAGATCATT TGTCCTATTT CACTTTCATT GGAAGAGTTG CTGGGCTGGC AGTATTTCAT
    GGGAAACTTC TAGATGGTTT CTTCATTCGA CCGTTTTATA AAATGATGCT GGGAAAGCAG
    ATAACTCTGA ATGACATGGA ATCTGTGGAT AGCGAATATT ACAACTCTTT GAAATGGATC
    TTAGAAAATG ACCCAACTGA ACTGGACCTT ATGTTCTGCA TTGATGAAGA GAACTTTGGG
    CAGACATATC AAGTGGATCT GAAGCCCAAC GGGTCAGAAA TAATGGTAAC GAACGAAAAC
    AAGAGAGAGT ACATCGACCT CGTCATCCAG TGGAGGTTCG TGAACAGGGT CCAGAAGCAG
    ATGAACGCCT TCCTGGAGGG ATTCACGGAA CTGCTCCCTA TTGATTTGAT TAAAATCTTT
    GATGAAAATG AACTGGAGTT GCTGATGTGT GGCCTTGGCG ACGTGGATGT GAACGACTGG
    CGACAACATT CTATTTACAA GAATGGCTAC TGCCCGAACC ACCCCGTGAT CCAGTGGTTC
    TGGAAGGCTG TGCTGCTGAT GGACGCCGAG AAGCGCATCC GGTTGCTGCA GTTTGTCACA
    GGGACGTCCC GAGTACCTAT GAATGGATTT GCCGAGCTTT ATGGTTCCAA TGGTCCTCAG
    CTGTTTACAA TAGAGCAATG GGGAAGTCCT GAAAAACTGC CCAGAGCTCA CACGTGCTTT
    AATCGCCTTG ACTTACCTCC GTATGAAACC TTCGAAGATT TACGAGAGAA GCTCCTCATG
    GCTGTGGAAA ACGCCCAAGG CTTCGAGGGG GTGGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005199811.3
    CDS556..3114
    Translation

    Target ORF information:

    RefSeq Version XM_005199754.3
    Organism Bos taurus(cattle)
    Definition Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005199754.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGCCATCAGT GGCCTATGTA
    CACACCACGC CGGGCCTGCC CTCGGGCTGG GAAGAGAGGA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCTATCAT GCAGCTCGCA
    GAAGACGGCG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CCGCCCCGCT GGAGGGCGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GATACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAGA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA CAACACAAAG
    ACCACGACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ACATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGCTGGG AAGAAAGGAT CCACTTGGAT
    GGCCGGACGT TTTACATTGA CCACAGCAGC CAGGTGTTAT GTGGCGAGGA CGATGCCAGA
    GACTCAGTGC CCTCGTACGA TAGCAAAATT ACTCAGTGGG AAGACCCAAG ACTGCAGAAC
    CCAGCTATTA CTGGTCCGGC TGTTCCTTAC TCCAGAGAAT TTAAGCAGAA ATATGACTAC
    TTTAGAAAGA AATTAAAGAA ACCTGCGGAT ATTCCAAATA GGTTTGAAAT GAAACTTCAC
    AGAAATAACA TATTTGAAGA GTCCTATCGG AGAATCATGT CTGTGAAAAG ACCAGATGTC
    CTGAAAGCTA GGCTGTGGAT TGAGTTTGAA TCGGAGAAAG GTCTGGACTA CGGGGGCGTG
    GCCAGAGAGT GGTTCTTCCT ACTGTCCAAG GAGATGTTCA ACCCTTACTA CGGCCTCTTC
    GAGTATTCGG CAACGGACAA CTACACACTT CAGATCAATC CCAATTCAGG CCTCTGTAAT
    GAAGATCATT TGTCCTATTT CACTTTCATT GGAAGAGTTG CTGGGCTGGC AGTATTTCAT
    GGGAAACTTC TAGATGGTTT CTTCATTCGA CCGTTTTATA AAATGATGCT GGGAAAGCAG
    ATAACTCTGA ATGACATGGA ATCTGTGGAT AGCGAATATT ACAACTCTTT GAAATGGATC
    TTAGAAAATG ACCCAACTGA ACTGGACCTT ATGTTCTGCA TTGATGAAGA GAACTTTGGG
    CAGACATATC AAGTGGATCT GAAGCCCAAC GGGTCAGAAA TAATGGTAAC GAACGAAAAC
    AAGAGAGAGT ACATCGACCT CGTCATCCAG TGGAGGTTCG TGAACAGGGT CCAGAAGCAG
    ATGAACGCCT TCCTGGAGGG ATTCACGGAA CTGCTCCCTA TTGATTTGAT TAAAATCTTT
    GATGAAAATG AACTGGAGTT GCTGATGTGT GGCCTTGGCG ACGTGGATGT GAACGACTGG
    CGACAACATT CTATTTACAA GAATGGCTAC TGCCCGAACC ACCCCGTGAT CCAGTGGTTC
    TGGAAGGCTG TGCTGCTGAT GGACGCCGAG AAGCGCATCC GGTTGCTGCA GTTTGTCACA
    GGGACGTCCC GAGTACCTAT GAATGGATTT GCCGAGCTTT ATGGTTCCAA TGGTCCTCAG
    CTGTTTACAA TAGAGCAATG GGGAAGTCCT GAAAAACTGC CCAGAGCTCA CACGTGCTTT
    AATCGCCTTG ACTTACCTCC GTATGAAACC TTCGAAGATT TACGAGAGAA GCTCCTCATG
    GCTGTGGAAA ACGCCCAAGG CTTCGAGGGG GTGGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88366
    Clone ID Related Accession (Same CDS sequence) XM_024984482.1 , XM_005199754.3 , XM_005224352.4 , XM_005224352.3
    Accession Version XM_005224352.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2559bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005224352.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGCCATCAGT GGCCTATGTA
    CACACCACGC CGGGCCTGCC CTCGGGCTGG GAAGAGAGGA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCTATCAT GCAGCTCGCA
    GAAGACGGCG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CCGCCCCGCT GGAGGGCGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GATACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAGA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA CAACACAAAG
    ACCACGACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ACATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGCTGGG AAGAAAGGAT CCACTTGGAT
    GGCCGGACGT TTTACATTGA CCACAGCAGC CAGGTGTTAT GTGGCGAGGA CGATGCCAGA
    GACTCAGTGC CCTCGTACGA TAGCAAAATT ACTCAGTGGG AAGACCCAAG ACTGCAGAAC
    CCAGCTATTA CTGGTCCGGC TGTTCCTTAC TCCAGAGAAT TTAAGCAGAA ATATGACTAC
    TTTAGAAAGA AATTAAAGAA ACCTGCGGAT ATTCCAAATA GGTTTGAAAT GAAACTTCAC
    AGAAATAACA TATTTGAAGA GTCCTATCGG AGAATCATGT CTGTGAAAAG ACCAGATGTC
    CTGAAAGCTA GGCTGTGGAT TGAGTTTGAA TCGGAGAAAG GTCTGGACTA CGGGGGCGTG
    GCCAGAGAGT GGTTCTTCCT ACTGTCCAAG GAGATGTTCA ACCCTTACTA CGGCCTCTTC
    GAGTATTCGG CAACGGACAA CTACACACTT CAGATCAATC CCAATTCAGG CCTCTGTAAT
    GAAGATCATT TGTCCTATTT CACTTTCATT GGAAGAGTTG CTGGGCTGGC AGTATTTCAT
    GGGAAACTTC TAGATGGTTT CTTCATTCGA CCGTTTTATA AAATGATGCT GGGAAAGCAG
    ATAACTCTGA ATGACATGGA ATCTGTGGAT AGCGAATATT ACAACTCTTT GAAATGGATC
    TTAGAAAATG ACCCAACTGA ACTGGACCTT ATGTTCTGCA TTGATGAAGA GAACTTTGGG
    CAGACATATC AAGTGGATCT GAAGCCCAAC GGGTCAGAAA TAATGGTAAC GAACGAAAAC
    AAGAGAGAGT ACATCGACCT CGTCATCCAG TGGAGGTTCG TGAACAGGGT CCAGAAGCAG
    ATGAACGCCT TCCTGGAGGG ATTCACGGAA CTGCTCCCTA TTGATTTGAT TAAAATCTTT
    GATGAAAATG AACTGGAGTT GCTGATGTGT GGCCTTGGCG ACGTGGATGT GAACGACTGG
    CGACAACATT CTATTTACAA GAATGGCTAC TGCCCGAACC ACCCCGTGAT CCAGTGGTTC
    TGGAAGGCTG TGCTGCTGAT GGACGCCGAG AAGCGCATCC GGTTGCTGCA GTTTGTCACA
    GGGACGTCCC GAGTACCTAT GAATGGATTT GCCGAGCTTT ATGGTTCCAA TGGTCCTCAG
    CTGTTTACAA TAGAGCAATG GGGAAGTCCT GAAAAACTGC CCAGAGCTCA CACGTGCTTT
    AATCGCCTTG ACTTACCTCC GTATGAAACC TTCGAAGATT TACGAGAGAA GCTCCTCATG
    GCTGTGGAAA ACGCCCAAGG CTTCGAGGGG GTGGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005224409.3
    CDS554..3112
    Translation

    Target ORF information:

    RefSeq Version XM_005224352.4
    Organism Bos taurus(cattle)
    Definition Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005224352.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGCCATCAGT GGCCTATGTA
    CACACCACGC CGGGCCTGCC CTCGGGCTGG GAAGAGAGGA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCTATCAT GCAGCTCGCA
    GAAGACGGCG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CCGCCCCGCT GGAGGGCGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GATACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAGA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA CAACACAAAG
    ACCACGACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ACATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGCTGGG AAGAAAGGAT CCACTTGGAT
    GGCCGGACGT TTTACATTGA CCACAGCAGC CAGGTGTTAT GTGGCGAGGA CGATGCCAGA
    GACTCAGTGC CCTCGTACGA TAGCAAAATT ACTCAGTGGG AAGACCCAAG ACTGCAGAAC
    CCAGCTATTA CTGGTCCGGC TGTTCCTTAC TCCAGAGAAT TTAAGCAGAA ATATGACTAC
    TTTAGAAAGA AATTAAAGAA ACCTGCGGAT ATTCCAAATA GGTTTGAAAT GAAACTTCAC
    AGAAATAACA TATTTGAAGA GTCCTATCGG AGAATCATGT CTGTGAAAAG ACCAGATGTC
    CTGAAAGCTA GGCTGTGGAT TGAGTTTGAA TCGGAGAAAG GTCTGGACTA CGGGGGCGTG
    GCCAGAGAGT GGTTCTTCCT ACTGTCCAAG GAGATGTTCA ACCCTTACTA CGGCCTCTTC
    GAGTATTCGG CAACGGACAA CTACACACTT CAGATCAATC CCAATTCAGG CCTCTGTAAT
    GAAGATCATT TGTCCTATTT CACTTTCATT GGAAGAGTTG CTGGGCTGGC AGTATTTCAT
    GGGAAACTTC TAGATGGTTT CTTCATTCGA CCGTTTTATA AAATGATGCT GGGAAAGCAG
    ATAACTCTGA ATGACATGGA ATCTGTGGAT AGCGAATATT ACAACTCTTT GAAATGGATC
    TTAGAAAATG ACCCAACTGA ACTGGACCTT ATGTTCTGCA TTGATGAAGA GAACTTTGGG
    CAGACATATC AAGTGGATCT GAAGCCCAAC GGGTCAGAAA TAATGGTAAC GAACGAAAAC
    AAGAGAGAGT ACATCGACCT CGTCATCCAG TGGAGGTTCG TGAACAGGGT CCAGAAGCAG
    ATGAACGCCT TCCTGGAGGG ATTCACGGAA CTGCTCCCTA TTGATTTGAT TAAAATCTTT
    GATGAAAATG AACTGGAGTT GCTGATGTGT GGCCTTGGCG ACGTGGATGT GAACGACTGG
    CGACAACATT CTATTTACAA GAATGGCTAC TGCCCGAACC ACCCCGTGAT CCAGTGGTTC
    TGGAAGGCTG TGCTGCTGAT GGACGCCGAG AAGCGCATCC GGTTGCTGCA GTTTGTCACA
    GGGACGTCCC GAGTACCTAT GAATGGATTT GCCGAGCTTT ATGGTTCCAA TGGTCCTCAG
    CTGTTTACAA TAGAGCAATG GGGAAGTCCT GAAAAACTGC CCAGAGCTCA CACGTGCTTT
    AATCGCCTTG ACTTACCTCC GTATGAAACC TTCGAAGATT TACGAGAGAA GCTCCTCATG
    GCTGTGGAAA ACGCCCAAGG CTTCGAGGGG GTGGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88366
    Clone ID Related Accession (Same CDS sequence) XM_024984482.1 , XM_005199754.3 , XM_005224352.4 , XM_005224352.3
    Accession Version XM_005224352.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2559bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104569.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005224352.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGCCATCAGT GGCCTATGTA
    CACACCACGC CGGGCCTGCC CTCGGGCTGG GAAGAGAGGA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCTATCAT GCAGCTCGCA
    GAAGACGGCG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CCGCCCCGCT GGAGGGCGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GATACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAGA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA CAACACAAAG
    ACCACGACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ACATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGCTGGG AAGAAAGGAT CCACTTGGAT
    GGCCGGACGT TTTACATTGA CCACAGCAGC CAGGTGTTAT GTGGCGAGGA CGATGCCAGA
    GACTCAGTGC CCTCGTACGA TAGCAAAATT ACTCAGTGGG AAGACCCAAG ACTGCAGAAC
    CCAGCTATTA CTGGTCCGGC TGTTCCTTAC TCCAGAGAAT TTAAGCAGAA ATATGACTAC
    TTTAGAAAGA AATTAAAGAA ACCTGCGGAT ATTCCAAATA GGTTTGAAAT GAAACTTCAC
    AGAAATAACA TATTTGAAGA GTCCTATCGG AGAATCATGT CTGTGAAAAG ACCAGATGTC
    CTGAAAGCTA GGCTGTGGAT TGAGTTTGAA TCGGAGAAAG GTCTGGACTA CGGGGGCGTG
    GCCAGAGAGT GGTTCTTCCT ACTGTCCAAG GAGATGTTCA ACCCTTACTA CGGCCTCTTC
    GAGTATTCGG CAACGGACAA CTACACACTT CAGATCAATC CCAATTCAGG CCTCTGTAAT
    GAAGATCATT TGTCCTATTT CACTTTCATT GGAAGAGTTG CTGGGCTGGC AGTATTTCAT
    GGGAAACTTC TAGATGGTTT CTTCATTCGA CCGTTTTATA AAATGATGCT GGGAAAGCAG
    ATAACTCTGA ATGACATGGA ATCTGTGGAT AGCGAATATT ACAACTCTTT GAAATGGATC
    TTAGAAAATG ACCCAACTGA ACTGGACCTT ATGTTCTGCA TTGATGAAGA GAACTTTGGG
    CAGACATATC AAGTGGATCT GAAGCCCAAC GGGTCAGAAA TAATGGTAAC GAACGAAAAC
    AAGAGAGAGT ACATCGACCT CGTCATCCAG TGGAGGTTCG TGAACAGGGT CCAGAAGCAG
    ATGAACGCCT TCCTGGAGGG ATTCACGGAA CTGCTCCCTA TTGATTTGAT TAAAATCTTT
    GATGAAAATG AACTGGAGTT GCTGATGTGT GGCCTTGGCG ACGTGGATGT GAACGACTGG
    CGACAACATT CTATTTACAA GAATGGCTAC TGCCCGAACC ACCCCGTGAT CCAGTGGTTC
    TGGAAGGCTG TGCTGCTGAT GGACGCCGAG AAGCGCATCC GGTTGCTGCA GTTTGTCACA
    GGGACGTCCC GAGTACCTAT GAATGGATTT GCCGAGCTTT ATGGTTCCAA TGGTCCTCAG
    CTGTTTACAA TAGAGCAATG GGGAAGTCCT GAAAAACTGC CCAGAGCTCA CACGTGCTTT
    AATCGCCTTG ACTTACCTCC GTATGAAACC TTCGAAGATT TACGAGAGAA GCTCCTCATG
    GCTGTGGAAA ACGCCCAAGG CTTCGAGGGG GTGGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005224409.3
    CDS556..3114
    Translation

    Target ORF information:

    RefSeq Version XM_005224352.3
    Organism Bos taurus(cattle)
    Definition Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005224352.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGCCATCAGT GGCCTATGTA
    CACACCACGC CGGGCCTGCC CTCGGGCTGG GAAGAGAGGA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCTATCAT GCAGCTCGCA
    GAAGACGGCG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CCGCCCCGCT GGAGGGCGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GATACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAGA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA CAACACAAAG
    ACCACGACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ACATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGCTGGG AAGAAAGGAT CCACTTGGAT
    GGCCGGACGT TTTACATTGA CCACAGCAGC CAGGTGTTAT GTGGCGAGGA CGATGCCAGA
    GACTCAGTGC CCTCGTACGA TAGCAAAATT ACTCAGTGGG AAGACCCAAG ACTGCAGAAC
    CCAGCTATTA CTGGTCCGGC TGTTCCTTAC TCCAGAGAAT TTAAGCAGAA ATATGACTAC
    TTTAGAAAGA AATTAAAGAA ACCTGCGGAT ATTCCAAATA GGTTTGAAAT GAAACTTCAC
    AGAAATAACA TATTTGAAGA GTCCTATCGG AGAATCATGT CTGTGAAAAG ACCAGATGTC
    CTGAAAGCTA GGCTGTGGAT TGAGTTTGAA TCGGAGAAAG GTCTGGACTA CGGGGGCGTG
    GCCAGAGAGT GGTTCTTCCT ACTGTCCAAG GAGATGTTCA ACCCTTACTA CGGCCTCTTC
    GAGTATTCGG CAACGGACAA CTACACACTT CAGATCAATC CCAATTCAGG CCTCTGTAAT
    GAAGATCATT TGTCCTATTT CACTTTCATT GGAAGAGTTG CTGGGCTGGC AGTATTTCAT
    GGGAAACTTC TAGATGGTTT CTTCATTCGA CCGTTTTATA AAATGATGCT GGGAAAGCAG
    ATAACTCTGA ATGACATGGA ATCTGTGGAT AGCGAATATT ACAACTCTTT GAAATGGATC
    TTAGAAAATG ACCCAACTGA ACTGGACCTT ATGTTCTGCA TTGATGAAGA GAACTTTGGG
    CAGACATATC AAGTGGATCT GAAGCCCAAC GGGTCAGAAA TAATGGTAAC GAACGAAAAC
    AAGAGAGAGT ACATCGACCT CGTCATCCAG TGGAGGTTCG TGAACAGGGT CCAGAAGCAG
    ATGAACGCCT TCCTGGAGGG ATTCACGGAA CTGCTCCCTA TTGATTTGAT TAAAATCTTT
    GATGAAAATG AACTGGAGTT GCTGATGTGT GGCCTTGGCG ACGTGGATGT GAACGACTGG
    CGACAACATT CTATTTACAA GAATGGCTAC TGCCCGAACC ACCCCGTGAT CCAGTGGTTC
    TGGAAGGCTG TGCTGCTGAT GGACGCCGAG AAGCGCATCC GGTTGCTGCA GTTTGTCACA
    GGGACGTCCC GAGTACCTAT GAATGGATTT GCCGAGCTTT ATGGTTCCAA TGGTCCTCAG
    CTGTTTACAA TAGAGCAATG GGGAAGTCCT GAAAAACTGC CCAGAGCTCA CACGTGCTTT
    AATCGCCTTG ACTTACCTCC GTATGAAACC TTCGAAGATT TACGAGAGAA GCTCCTCATG
    GCTGTGGAAA ACGCCCAAGG CTTCGAGGGG GTGGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88367
    Clone ID Related Accession (Same CDS sequence) XM_015469183.1 , XM_015460153.2 , XM_015460153.1
    Accession Version XM_015469183.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2517bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646636.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGCCCAGTGC CCCAGCTGGG
    AGAGCACGTT CATCAACTGT CACTGGTGGT GAGGCGCCAA CGCCATCAGT GGCCTATGTA
    CACACCACGC CGGGCCTGCC CTCGGGCTGG GAAGAGAGGA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCTATCAT GCAGCTCGCA
    GAAGACGGCG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CCGCCCCGCT GGAGGGCGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GATACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAGA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA CAACACAAAG
    ACCACGACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ACATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGCTGGG AAGAAAGGAT CCACTTGGAT
    GGCCGGACGT TTTACATTGA CCACAGCAGC CAGGTGTTAT GTGGCGAGGA CGATGCCAGA
    GACTCAGTGC CCTCGTACGA TAGCAAAATT ACTCAGTGGG AAGACCCAAG ACTGCAGAAC
    CCAGCTATTA CTGGTCCGGC TGTTCCTTAC TCCAGAGAAT TTAAGCAGAA ATATGACTAC
    TTTAGAAAGA AATTAAAGAA ACCTGCGGAT ATTCCAAATA GGTTTGAAAT GAAACTTCAC
    AGAAATAACA TATTTGAAGA GTCCTATCGG AGAATCATGT CTGTGAAAAG ACCAGATGTC
    CTGAAAGCTA GGCTGTGGAT TGAGTTTGAA TCGGAGAAAG GTCTGGACTA CGGGGGCGTG
    GCCAGAGAGT GGTTCTTCCT ACTGTCCAAG GAGATGTTCA ACCCTTACTA CGGCCTCTTC
    GAGTATTCGG CAACGGACAA CTACACACTT CAGATCAATC CCAATTCAGG CCTCTGTAAT
    GAAGATCATT TGTCCTATTT CACTTTCATT GGAAGAGTTG CTGGGCTGGC AGTATTTCAT
    GGGAAACTTC TAGATGGTTT CTTCATTCGA CCGTTTTATA AAATGATGCT GGGAAAGCAG
    ATAACTCTGA ATGACATGGA ATCTGTGGAT AGCGAATATT ACAACTCTTT GAAATGGATC
    TTAGAAAATG ACCCAACTGA ACTGGACCTT ATGTTCTGCA TTGATGAAGA GAACTTTGGG
    CAGACATATC AAGTGGATCT GAAGCCCAAC GGGTCAGAAA TAATGGTAAC GAACGAAAAC
    AAGAGAGAGT ACATCGACCT CGTCATCCAG TGGAGGTTCG TGAACAGGGT CCAGAAGCAG
    ATGAACGCCT TCCTGGAGGG ATTCACGGAA CTGCTCCCTA TTGATTTGAT TAAAATCTTT
    GATGAAAATG AACTGGAGTT GCTGATGTGT GGCCTTGGCG ACGTGGATGT GAACGACTGG
    CGACAACATT CTATTTACAA GAATGGCTAC TGCCCGAACC ACCCCGTGAT CCAGTGGTTC
    TGGAAGGCTG TGCTGCTGAT GGACGCCGAG AAGCGCATCC GGTTGCTGCA GTTTGTCACA
    GGGACGTCCC GAGTACCTAT GAATGGATTT GCCGAGCTTT ATGGTTCCAA TGGTCCTCAG
    CTGTTTACAA TAGAGCAATG GGGAAGTCCT GAAAAACTGC CCAGAGCTCA CACGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015324669.1
    CDS562..3078
    Translation

    Target ORF information:

    RefSeq Version XM_015469183.1
    Organism Bos taurus(cattle)
    Definition Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015469183.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGCCCAGTGC CCCAGCTGGG
    AGAGCACGTT CATCAACTGT CACTGGTGGT GAGGCGCCAA CGCCATCAGT GGCCTATGTA
    CACACCACGC CGGGCCTGCC CTCGGGCTGG GAAGAGAGGA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCTATCAT GCAGCTCGCA
    GAAGACGGCG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CCGCCCCGCT GGAGGGCGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GATACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAGA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA CAACACAAAG
    ACCACGACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ACATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGCTGGG AAGAAAGGAT CCACTTGGAT
    GGCCGGACGT TTTACATTGA CCACAGCAGC CAGGTGTTAT GTGGCGAGGA CGATGCCAGA
    GACTCAGTGC CCTCGTACGA TAGCAAAATT ACTCAGTGGG AAGACCCAAG ACTGCAGAAC
    CCAGCTATTA CTGGTCCGGC TGTTCCTTAC TCCAGAGAAT TTAAGCAGAA ATATGACTAC
    TTTAGAAAGA AATTAAAGAA ACCTGCGGAT ATTCCAAATA GGTTTGAAAT GAAACTTCAC
    AGAAATAACA TATTTGAAGA GTCCTATCGG AGAATCATGT CTGTGAAAAG ACCAGATGTC
    CTGAAAGCTA GGCTGTGGAT TGAGTTTGAA TCGGAGAAAG GTCTGGACTA CGGGGGCGTG
    GCCAGAGAGT GGTTCTTCCT ACTGTCCAAG GAGATGTTCA ACCCTTACTA CGGCCTCTTC
    GAGTATTCGG CAACGGACAA CTACACACTT CAGATCAATC CCAATTCAGG CCTCTGTAAT
    GAAGATCATT TGTCCTATTT CACTTTCATT GGAAGAGTTG CTGGGCTGGC AGTATTTCAT
    GGGAAACTTC TAGATGGTTT CTTCATTCGA CCGTTTTATA AAATGATGCT GGGAAAGCAG
    ATAACTCTGA ATGACATGGA ATCTGTGGAT AGCGAATATT ACAACTCTTT GAAATGGATC
    TTAGAAAATG ACCCAACTGA ACTGGACCTT ATGTTCTGCA TTGATGAAGA GAACTTTGGG
    CAGACATATC AAGTGGATCT GAAGCCCAAC GGGTCAGAAA TAATGGTAAC GAACGAAAAC
    AAGAGAGAGT ACATCGACCT CGTCATCCAG TGGAGGTTCG TGAACAGGGT CCAGAAGCAG
    ATGAACGCCT TCCTGGAGGG ATTCACGGAA CTGCTCCCTA TTGATTTGAT TAAAATCTTT
    GATGAAAATG AACTGGAGTT GCTGATGTGT GGCCTTGGCG ACGTGGATGT GAACGACTGG
    CGACAACATT CTATTTACAA GAATGGCTAC TGCCCGAACC ACCCCGTGAT CCAGTGGTTC
    TGGAAGGCTG TGCTGCTGAT GGACGCCGAG AAGCGCATCC GGTTGCTGCA GTTTGTCACA
    GGGACGTCCC GAGTACCTAT GAATGGATTT GCCGAGCTTT ATGGTTCCAA TGGTCCTCAG
    CTGTTTACAA TAGAGCAATG GGGAAGTCCT GAAAAACTGC CCAGAGCTCA CACGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88367
    Clone ID Related Accession (Same CDS sequence) XM_015469183.1 , XM_015460153.2 , XM_015460153.1
    Accession Version XM_015460153.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2517bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015460153.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGCCCAGTGC CCCAGCTGGG
    AGAGCACGTT CATCAACTGT CACTGGTGGT GAGGCGCCAA CGCCATCAGT GGCCTATGTA
    CACACCACGC CGGGCCTGCC CTCGGGCTGG GAAGAGAGGA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCTATCAT GCAGCTCGCA
    GAAGACGGCG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CCGCCCCGCT GGAGGGCGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GATACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAGA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA CAACACAAAG
    ACCACGACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ACATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGCTGGG AAGAAAGGAT CCACTTGGAT
    GGCCGGACGT TTTACATTGA CCACAGCAGC CAGGTGTTAT GTGGCGAGGA CGATGCCAGA
    GACTCAGTGC CCTCGTACGA TAGCAAAATT ACTCAGTGGG AAGACCCAAG ACTGCAGAAC
    CCAGCTATTA CTGGTCCGGC TGTTCCTTAC TCCAGAGAAT TTAAGCAGAA ATATGACTAC
    TTTAGAAAGA AATTAAAGAA ACCTGCGGAT ATTCCAAATA GGTTTGAAAT GAAACTTCAC
    AGAAATAACA TATTTGAAGA GTCCTATCGG AGAATCATGT CTGTGAAAAG ACCAGATGTC
    CTGAAAGCTA GGCTGTGGAT TGAGTTTGAA TCGGAGAAAG GTCTGGACTA CGGGGGCGTG
    GCCAGAGAGT GGTTCTTCCT ACTGTCCAAG GAGATGTTCA ACCCTTACTA CGGCCTCTTC
    GAGTATTCGG CAACGGACAA CTACACACTT CAGATCAATC CCAATTCAGG CCTCTGTAAT
    GAAGATCATT TGTCCTATTT CACTTTCATT GGAAGAGTTG CTGGGCTGGC AGTATTTCAT
    GGGAAACTTC TAGATGGTTT CTTCATTCGA CCGTTTTATA AAATGATGCT GGGAAAGCAG
    ATAACTCTGA ATGACATGGA ATCTGTGGAT AGCGAATATT ACAACTCTTT GAAATGGATC
    TTAGAAAATG ACCCAACTGA ACTGGACCTT ATGTTCTGCA TTGATGAAGA GAACTTTGGG
    CAGACATATC AAGTGGATCT GAAGCCCAAC GGGTCAGAAA TAATGGTAAC GAACGAAAAC
    AAGAGAGAGT ACATCGACCT CGTCATCCAG TGGAGGTTCG TGAACAGGGT CCAGAAGCAG
    ATGAACGCCT TCCTGGAGGG ATTCACGGAA CTGCTCCCTA TTGATTTGAT TAAAATCTTT
    GATGAAAATG AACTGGAGTT GCTGATGTGT GGCCTTGGCG ACGTGGATGT GAACGACTGG
    CGACAACATT CTATTTACAA GAATGGCTAC TGCCCGAACC ACCCCGTGAT CCAGTGGTTC
    TGGAAGGCTG TGCTGCTGAT GGACGCCGAG AAGCGCATCC GGTTGCTGCA GTTTGTCACA
    GGGACGTCCC GAGTACCTAT GAATGGATTT GCCGAGCTTT ATGGTTCCAA TGGTCCTCAG
    CTGTTTACAA TAGAGCAATG GGGAAGTCCT GAAAAACTGC CCAGAGCTCA CACGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015315639.1
    CDS562..3078
    Translation

    Target ORF information:

    RefSeq Version XM_015460153.2
    Organism Bos taurus(cattle)
    Definition Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015460153.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGCCCAGTGC CCCAGCTGGG
    AGAGCACGTT CATCAACTGT CACTGGTGGT GAGGCGCCAA CGCCATCAGT GGCCTATGTA
    CACACCACGC CGGGCCTGCC CTCGGGCTGG GAAGAGAGGA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCTATCAT GCAGCTCGCA
    GAAGACGGCG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CCGCCCCGCT GGAGGGCGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GATACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAGA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA CAACACAAAG
    ACCACGACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ACATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGCTGGG AAGAAAGGAT CCACTTGGAT
    GGCCGGACGT TTTACATTGA CCACAGCAGC CAGGTGTTAT GTGGCGAGGA CGATGCCAGA
    GACTCAGTGC CCTCGTACGA TAGCAAAATT ACTCAGTGGG AAGACCCAAG ACTGCAGAAC
    CCAGCTATTA CTGGTCCGGC TGTTCCTTAC TCCAGAGAAT TTAAGCAGAA ATATGACTAC
    TTTAGAAAGA AATTAAAGAA ACCTGCGGAT ATTCCAAATA GGTTTGAAAT GAAACTTCAC
    AGAAATAACA TATTTGAAGA GTCCTATCGG AGAATCATGT CTGTGAAAAG ACCAGATGTC
    CTGAAAGCTA GGCTGTGGAT TGAGTTTGAA TCGGAGAAAG GTCTGGACTA CGGGGGCGTG
    GCCAGAGAGT GGTTCTTCCT ACTGTCCAAG GAGATGTTCA ACCCTTACTA CGGCCTCTTC
    GAGTATTCGG CAACGGACAA CTACACACTT CAGATCAATC CCAATTCAGG CCTCTGTAAT
    GAAGATCATT TGTCCTATTT CACTTTCATT GGAAGAGTTG CTGGGCTGGC AGTATTTCAT
    GGGAAACTTC TAGATGGTTT CTTCATTCGA CCGTTTTATA AAATGATGCT GGGAAAGCAG
    ATAACTCTGA ATGACATGGA ATCTGTGGAT AGCGAATATT ACAACTCTTT GAAATGGATC
    TTAGAAAATG ACCCAACTGA ACTGGACCTT ATGTTCTGCA TTGATGAAGA GAACTTTGGG
    CAGACATATC AAGTGGATCT GAAGCCCAAC GGGTCAGAAA TAATGGTAAC GAACGAAAAC
    AAGAGAGAGT ACATCGACCT CGTCATCCAG TGGAGGTTCG TGAACAGGGT CCAGAAGCAG
    ATGAACGCCT TCCTGGAGGG ATTCACGGAA CTGCTCCCTA TTGATTTGAT TAAAATCTTT
    GATGAAAATG AACTGGAGTT GCTGATGTGT GGCCTTGGCG ACGTGGATGT GAACGACTGG
    CGACAACATT CTATTTACAA GAATGGCTAC TGCCCGAACC ACCCCGTGAT CCAGTGGTTC
    TGGAAGGCTG TGCTGCTGAT GGACGCCGAG AAGCGCATCC GGTTGCTGCA GTTTGTCACA
    GGGACGTCCC GAGTACCTAT GAATGGATTT GCCGAGCTTT ATGGTTCCAA TGGTCCTCAG
    CTGTTTACAA TAGAGCAATG GGGAAGTCCT GAAAAACTGC CCAGAGCTCA CACGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88367
    Clone ID Related Accession (Same CDS sequence) XM_015469183.1 , XM_015460153.2 , XM_015460153.1
    Accession Version XM_015460153.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2517bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104569.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGCCCAGTGC CCCAGCTGGG
    AGAGCACGTT CATCAACTGT CACTGGTGGT GAGGCGCCAA CGCCATCAGT GGCCTATGTA
    CACACCACGC CGGGCCTGCC CTCGGGCTGG GAAGAGAGGA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCTATCAT GCAGCTCGCA
    GAAGACGGCG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CCGCCCCGCT GGAGGGCGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GATACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAGA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA CAACACAAAG
    ACCACGACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ACATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGCTGGG AAGAAAGGAT CCACTTGGAT
    GGCCGGACGT TTTACATTGA CCACAGCAGC CAGGTGTTAT GTGGCGAGGA CGATGCCAGA
    GACTCAGTGC CCTCGTACGA TAGCAAAATT ACTCAGTGGG AAGACCCAAG ACTGCAGAAC
    CCAGCTATTA CTGGTCCGGC TGTTCCTTAC TCCAGAGAAT TTAAGCAGAA ATATGACTAC
    TTTAGAAAGA AATTAAAGAA ACCTGCGGAT ATTCCAAATA GGTTTGAAAT GAAACTTCAC
    AGAAATAACA TATTTGAAGA GTCCTATCGG AGAATCATGT CTGTGAAAAG ACCAGATGTC
    CTGAAAGCTA GGCTGTGGAT TGAGTTTGAA TCGGAGAAAG GTCTGGACTA CGGGGGCGTG
    GCCAGAGAGT GGTTCTTCCT ACTGTCCAAG GAGATGTTCA ACCCTTACTA CGGCCTCTTC
    GAGTATTCGG CAACGGACAA CTACACACTT CAGATCAATC CCAATTCAGG CCTCTGTAAT
    GAAGATCATT TGTCCTATTT CACTTTCATT GGAAGAGTTG CTGGGCTGGC AGTATTTCAT
    GGGAAACTTC TAGATGGTTT CTTCATTCGA CCGTTTTATA AAATGATGCT GGGAAAGCAG
    ATAACTCTGA ATGACATGGA ATCTGTGGAT AGCGAATATT ACAACTCTTT GAAATGGATC
    TTAGAAAATG ACCCAACTGA ACTGGACCTT ATGTTCTGCA TTGATGAAGA GAACTTTGGG
    CAGACATATC AAGTGGATCT GAAGCCCAAC GGGTCAGAAA TAATGGTAAC GAACGAAAAC
    AAGAGAGAGT ACATCGACCT CGTCATCCAG TGGAGGTTCG TGAACAGGGT CCAGAAGCAG
    ATGAACGCCT TCCTGGAGGG ATTCACGGAA CTGCTCCCTA TTGATTTGAT TAAAATCTTT
    GATGAAAATG AACTGGAGTT GCTGATGTGT GGCCTTGGCG ACGTGGATGT GAACGACTGG
    CGACAACATT CTATTTACAA GAATGGCTAC TGCCCGAACC ACCCCGTGAT CCAGTGGTTC
    TGGAAGGCTG TGCTGCTGAT GGACGCCGAG AAGCGCATCC GGTTGCTGCA GTTTGTCACA
    GGGACGTCCC GAGTACCTAT GAATGGATTT GCCGAGCTTT ATGGTTCCAA TGGTCCTCAG
    CTGTTTACAA TAGAGCAATG GGGAAGTCCT GAAAAACTGC CCAGAGCTCA CACGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015315639.1
    CDS562..3078
    Translation

    Target ORF information:

    RefSeq Version XM_015460153.1
    Organism Bos taurus(cattle)
    Definition Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015460153.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGCCCAGTGC CCCAGCTGGG
    AGAGCACGTT CATCAACTGT CACTGGTGGT GAGGCGCCAA CGCCATCAGT GGCCTATGTA
    CACACCACGC CGGGCCTGCC CTCGGGCTGG GAAGAGAGGA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCTATCAT GCAGCTCGCA
    GAAGACGGCG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CCGCCCCGCT GGAGGGCGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GATACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAGA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA CAACACAAAG
    ACCACGACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ACATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGCTGGG AAGAAAGGAT CCACTTGGAT
    GGCCGGACGT TTTACATTGA CCACAGCAGC CAGGTGTTAT GTGGCGAGGA CGATGCCAGA
    GACTCAGTGC CCTCGTACGA TAGCAAAATT ACTCAGTGGG AAGACCCAAG ACTGCAGAAC
    CCAGCTATTA CTGGTCCGGC TGTTCCTTAC TCCAGAGAAT TTAAGCAGAA ATATGACTAC
    TTTAGAAAGA AATTAAAGAA ACCTGCGGAT ATTCCAAATA GGTTTGAAAT GAAACTTCAC
    AGAAATAACA TATTTGAAGA GTCCTATCGG AGAATCATGT CTGTGAAAAG ACCAGATGTC
    CTGAAAGCTA GGCTGTGGAT TGAGTTTGAA TCGGAGAAAG GTCTGGACTA CGGGGGCGTG
    GCCAGAGAGT GGTTCTTCCT ACTGTCCAAG GAGATGTTCA ACCCTTACTA CGGCCTCTTC
    GAGTATTCGG CAACGGACAA CTACACACTT CAGATCAATC CCAATTCAGG CCTCTGTAAT
    GAAGATCATT TGTCCTATTT CACTTTCATT GGAAGAGTTG CTGGGCTGGC AGTATTTCAT
    GGGAAACTTC TAGATGGTTT CTTCATTCGA CCGTTTTATA AAATGATGCT GGGAAAGCAG
    ATAACTCTGA ATGACATGGA ATCTGTGGAT AGCGAATATT ACAACTCTTT GAAATGGATC
    TTAGAAAATG ACCCAACTGA ACTGGACCTT ATGTTCTGCA TTGATGAAGA GAACTTTGGG
    CAGACATATC AAGTGGATCT GAAGCCCAAC GGGTCAGAAA TAATGGTAAC GAACGAAAAC
    AAGAGAGAGT ACATCGACCT CGTCATCCAG TGGAGGTTCG TGAACAGGGT CCAGAAGCAG
    ATGAACGCCT TCCTGGAGGG ATTCACGGAA CTGCTCCCTA TTGATTTGAT TAAAATCTTT
    GATGAAAATG AACTGGAGTT GCTGATGTGT GGCCTTGGCG ACGTGGATGT GAACGACTGG
    CGACAACATT CTATTTACAA GAATGGCTAC TGCCCGAACC ACCCCGTGAT CCAGTGGTTC
    TGGAAGGCTG TGCTGCTGAT GGACGCCGAG AAGCGCATCC GGTTGCTGCA GTTTGTCACA
    GGGACGTCCC GAGTACCTAT GAATGGATTT GCCGAGCTTT ATGGTTCCAA TGGTCCTCAG
    CTGTTTACAA TAGAGCAATG GGGAAGTCCT GAAAAACTGC CCAGAGCTCA CACGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88368
    Clone ID Related Accession (Same CDS sequence) XM_005224353.4 , XM_005224353.3 , XM_024984483.1 , XM_005199755.3 , XM_024984485.1 , XM_015460159.1 , XM_015469188.1
    Accession Version XM_005224353.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2505bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005224353.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGCCATCAGT GGCCTATGTA
    CACACCACGC CGGGCCTGCC CTCGGGCTGG GAAGAGAGGA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCTATCAT GCAGCTCGCA
    GAAGACGGCG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CCGCCCCGCT GGAGGGCGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GATACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAGA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA CAACACAAAG
    ACCACGACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ACATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGCTGGG AAGAAAGGAT CCACTTGGAT
    GGCCGGACGT TTTACATTGA CCACAATAGC AAAATTACTC AGTGGGAAGA CCCAAGACTG
    CAGAACCCAG CTATTACTGG TCCGGCTGTT CCTTACTCCA GAGAATTTAA GCAGAAATAT
    GACTACTTTA GAAAGAAATT AAAGAAACCT GCGGATATTC CAAATAGGTT TGAAATGAAA
    CTTCACAGAA ATAACATATT TGAAGAGTCC TATCGGAGAA TCATGTCTGT GAAAAGACCA
    GATGTCCTGA AAGCTAGGCT GTGGATTGAG TTTGAATCGG AGAAAGGTCT GGACTACGGG
    GGCGTGGCCA GAGAGTGGTT CTTCCTACTG TCCAAGGAGA TGTTCAACCC TTACTACGGC
    CTCTTCGAGT ATTCGGCAAC GGACAACTAC ACACTTCAGA TCAATCCCAA TTCAGGCCTC
    TGTAATGAAG ATCATTTGTC CTATTTCACT TTCATTGGAA GAGTTGCTGG GCTGGCAGTA
    TTTCATGGGA AACTTCTAGA TGGTTTCTTC ATTCGACCGT TTTATAAAAT GATGCTGGGA
    AAGCAGATAA CTCTGAATGA CATGGAATCT GTGGATAGCG AATATTACAA CTCTTTGAAA
    TGGATCTTAG AAAATGACCC AACTGAACTG GACCTTATGT TCTGCATTGA TGAAGAGAAC
    TTTGGGCAGA CATATCAAGT GGATCTGAAG CCCAACGGGT CAGAAATAAT GGTAACGAAC
    GAAAACAAGA GAGAGTACAT CGACCTCGTC ATCCAGTGGA GGTTCGTGAA CAGGGTCCAG
    AAGCAGATGA ACGCCTTCCT GGAGGGATTC ACGGAACTGC TCCCTATTGA TTTGATTAAA
    ATCTTTGATG AAAATGAACT GGAGTTGCTG ATGTGTGGCC TTGGCGACGT GGATGTGAAC
    GACTGGCGAC AACATTCTAT TTACAAGAAT GGCTACTGCC CGAACCACCC CGTGATCCAG
    TGGTTCTGGA AGGCTGTGCT GCTGATGGAC GCCGAGAAGC GCATCCGGTT GCTGCAGTTT
    GTCACAGGGA CGTCCCGAGT ACCTATGAAT GGATTTGCCG AGCTTTATGG TTCCAATGGT
    CCTCAGCTGT TTACAATAGA GCAATGGGGA AGTCCTGAAA AACTGCCCAG AGCTCACACG
    TGCTTTAATC GCCTTGACTT ACCTCCGTAT GAAACCTTCG AAGATTTACG AGAGAAGCTC
    CTCATGGCTG TGGAAAACGC CCAAGGCTTC GAGGGGGTGG ATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005224410.3
    CDS547..3051
    Translation

    Target ORF information:

    RefSeq Version XM_005224353.4
    Organism Bos taurus(cattle)
    Definition Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005224353.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGCCATCAGT GGCCTATGTA
    CACACCACGC CGGGCCTGCC CTCGGGCTGG GAAGAGAGGA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCTATCAT GCAGCTCGCA
    GAAGACGGCG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CCGCCCCGCT GGAGGGCGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GATACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAGA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA CAACACAAAG
    ACCACGACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ACATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGCTGGG AAGAAAGGAT CCACTTGGAT
    GGCCGGACGT TTTACATTGA CCACAATAGC AAAATTACTC AGTGGGAAGA CCCAAGACTG
    CAGAACCCAG CTATTACTGG TCCGGCTGTT CCTTACTCCA GAGAATTTAA GCAGAAATAT
    GACTACTTTA GAAAGAAATT AAAGAAACCT GCGGATATTC CAAATAGGTT TGAAATGAAA
    CTTCACAGAA ATAACATATT TGAAGAGTCC TATCGGAGAA TCATGTCTGT GAAAAGACCA
    GATGTCCTGA AAGCTAGGCT GTGGATTGAG TTTGAATCGG AGAAAGGTCT GGACTACGGG
    GGCGTGGCCA GAGAGTGGTT CTTCCTACTG TCCAAGGAGA TGTTCAACCC TTACTACGGC
    CTCTTCGAGT ATTCGGCAAC GGACAACTAC ACACTTCAGA TCAATCCCAA TTCAGGCCTC
    TGTAATGAAG ATCATTTGTC CTATTTCACT TTCATTGGAA GAGTTGCTGG GCTGGCAGTA
    TTTCATGGGA AACTTCTAGA TGGTTTCTTC ATTCGACCGT TTTATAAAAT GATGCTGGGA
    AAGCAGATAA CTCTGAATGA CATGGAATCT GTGGATAGCG AATATTACAA CTCTTTGAAA
    TGGATCTTAG AAAATGACCC AACTGAACTG GACCTTATGT TCTGCATTGA TGAAGAGAAC
    TTTGGGCAGA CATATCAAGT GGATCTGAAG CCCAACGGGT CAGAAATAAT GGTAACGAAC
    GAAAACAAGA GAGAGTACAT CGACCTCGTC ATCCAGTGGA GGTTCGTGAA CAGGGTCCAG
    AAGCAGATGA ACGCCTTCCT GGAGGGATTC ACGGAACTGC TCCCTATTGA TTTGATTAAA
    ATCTTTGATG AAAATGAACT GGAGTTGCTG ATGTGTGGCC TTGGCGACGT GGATGTGAAC
    GACTGGCGAC AACATTCTAT TTACAAGAAT GGCTACTGCC CGAACCACCC CGTGATCCAG
    TGGTTCTGGA AGGCTGTGCT GCTGATGGAC GCCGAGAAGC GCATCCGGTT GCTGCAGTTT
    GTCACAGGGA CGTCCCGAGT ACCTATGAAT GGATTTGCCG AGCTTTATGG TTCCAATGGT
    CCTCAGCTGT TTACAATAGA GCAATGGGGA AGTCCTGAAA AACTGCCCAG AGCTCACACG
    TGCTTTAATC GCCTTGACTT ACCTCCGTAT GAAACCTTCG AAGATTTACG AGAGAAGCTC
    CTCATGGCTG TGGAAAACGC CCAAGGCTTC GAGGGGGTGG ATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88368
    Clone ID Related Accession (Same CDS sequence) XM_005224353.4 , XM_005224353.3 , XM_024984483.1 , XM_005199755.3 , XM_024984485.1 , XM_015460159.1 , XM_015469188.1
    Accession Version XM_005224353.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2505bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104569.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005224353.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGCCATCAGT GGCCTATGTA
    CACACCACGC CGGGCCTGCC CTCGGGCTGG GAAGAGAGGA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCTATCAT GCAGCTCGCA
    GAAGACGGCG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CCGCCCCGCT GGAGGGCGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GATACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAGA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA CAACACAAAG
    ACCACGACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ACATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGCTGGG AAGAAAGGAT CCACTTGGAT
    GGCCGGACGT TTTACATTGA CCACAATAGC AAAATTACTC AGTGGGAAGA CCCAAGACTG
    CAGAACCCAG CTATTACTGG TCCGGCTGTT CCTTACTCCA GAGAATTTAA GCAGAAATAT
    GACTACTTTA GAAAGAAATT AAAGAAACCT GCGGATATTC CAAATAGGTT TGAAATGAAA
    CTTCACAGAA ATAACATATT TGAAGAGTCC TATCGGAGAA TCATGTCTGT GAAAAGACCA
    GATGTCCTGA AAGCTAGGCT GTGGATTGAG TTTGAATCGG AGAAAGGTCT GGACTACGGG
    GGCGTGGCCA GAGAGTGGTT CTTCCTACTG TCCAAGGAGA TGTTCAACCC TTACTACGGC
    CTCTTCGAGT ATTCGGCAAC GGACAACTAC ACACTTCAGA TCAATCCCAA TTCAGGCCTC
    TGTAATGAAG ATCATTTGTC CTATTTCACT TTCATTGGAA GAGTTGCTGG GCTGGCAGTA
    TTTCATGGGA AACTTCTAGA TGGTTTCTTC ATTCGACCGT TTTATAAAAT GATGCTGGGA
    AAGCAGATAA CTCTGAATGA CATGGAATCT GTGGATAGCG AATATTACAA CTCTTTGAAA
    TGGATCTTAG AAAATGACCC AACTGAACTG GACCTTATGT TCTGCATTGA TGAAGAGAAC
    TTTGGGCAGA CATATCAAGT GGATCTGAAG CCCAACGGGT CAGAAATAAT GGTAACGAAC
    GAAAACAAGA GAGAGTACAT CGACCTCGTC ATCCAGTGGA GGTTCGTGAA CAGGGTCCAG
    AAGCAGATGA ACGCCTTCCT GGAGGGATTC ACGGAACTGC TCCCTATTGA TTTGATTAAA
    ATCTTTGATG AAAATGAACT GGAGTTGCTG ATGTGTGGCC TTGGCGACGT GGATGTGAAC
    GACTGGCGAC AACATTCTAT TTACAAGAAT GGCTACTGCC CGAACCACCC CGTGATCCAG
    TGGTTCTGGA AGGCTGTGCT GCTGATGGAC GCCGAGAAGC GCATCCGGTT GCTGCAGTTT
    GTCACAGGGA CGTCCCGAGT ACCTATGAAT GGATTTGCCG AGCTTTATGG TTCCAATGGT
    CCTCAGCTGT TTACAATAGA GCAATGGGGA AGTCCTGAAA AACTGCCCAG AGCTCACACG
    TGCTTTAATC GCCTTGACTT ACCTCCGTAT GAAACCTTCG AAGATTTACG AGAGAAGCTC
    CTCATGGCTG TGGAAAACGC CCAAGGCTTC GAGGGGGTGG ATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005224410.3
    CDS551..3055
    Translation

    Target ORF information:

    RefSeq Version XM_005224353.3
    Organism Bos taurus(cattle)
    Definition Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005224353.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGCCATCAGT GGCCTATGTA
    CACACCACGC CGGGCCTGCC CTCGGGCTGG GAAGAGAGGA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCTATCAT GCAGCTCGCA
    GAAGACGGCG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CCGCCCCGCT GGAGGGCGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GATACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAGA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA CAACACAAAG
    ACCACGACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ACATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGCTGGG AAGAAAGGAT CCACTTGGAT
    GGCCGGACGT TTTACATTGA CCACAATAGC AAAATTACTC AGTGGGAAGA CCCAAGACTG
    CAGAACCCAG CTATTACTGG TCCGGCTGTT CCTTACTCCA GAGAATTTAA GCAGAAATAT
    GACTACTTTA GAAAGAAATT AAAGAAACCT GCGGATATTC CAAATAGGTT TGAAATGAAA
    CTTCACAGAA ATAACATATT TGAAGAGTCC TATCGGAGAA TCATGTCTGT GAAAAGACCA
    GATGTCCTGA AAGCTAGGCT GTGGATTGAG TTTGAATCGG AGAAAGGTCT GGACTACGGG
    GGCGTGGCCA GAGAGTGGTT CTTCCTACTG TCCAAGGAGA TGTTCAACCC TTACTACGGC
    CTCTTCGAGT ATTCGGCAAC GGACAACTAC ACACTTCAGA TCAATCCCAA TTCAGGCCTC
    TGTAATGAAG ATCATTTGTC CTATTTCACT TTCATTGGAA GAGTTGCTGG GCTGGCAGTA
    TTTCATGGGA AACTTCTAGA TGGTTTCTTC ATTCGACCGT TTTATAAAAT GATGCTGGGA
    AAGCAGATAA CTCTGAATGA CATGGAATCT GTGGATAGCG AATATTACAA CTCTTTGAAA
    TGGATCTTAG AAAATGACCC AACTGAACTG GACCTTATGT TCTGCATTGA TGAAGAGAAC
    TTTGGGCAGA CATATCAAGT GGATCTGAAG CCCAACGGGT CAGAAATAAT GGTAACGAAC
    GAAAACAAGA GAGAGTACAT CGACCTCGTC ATCCAGTGGA GGTTCGTGAA CAGGGTCCAG
    AAGCAGATGA ACGCCTTCCT GGAGGGATTC ACGGAACTGC TCCCTATTGA TTTGATTAAA
    ATCTTTGATG AAAATGAACT GGAGTTGCTG ATGTGTGGCC TTGGCGACGT GGATGTGAAC
    GACTGGCGAC AACATTCTAT TTACAAGAAT GGCTACTGCC CGAACCACCC CGTGATCCAG
    TGGTTCTGGA AGGCTGTGCT GCTGATGGAC GCCGAGAAGC GCATCCGGTT GCTGCAGTTT
    GTCACAGGGA CGTCCCGAGT ACCTATGAAT GGATTTGCCG AGCTTTATGG TTCCAATGGT
    CCTCAGCTGT TTACAATAGA GCAATGGGGA AGTCCTGAAA AACTGCCCAG AGCTCACACG
    TGCTTTAATC GCCTTGACTT ACCTCCGTAT GAAACCTTCG AAGATTTACG AGAGAAGCTC
    CTCATGGCTG TGGAAAACGC CCAAGGCTTC GAGGGGGTGG ATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88368
    Clone ID Related Accession (Same CDS sequence) XM_005224353.4 , XM_005224353.3 , XM_024984483.1 , XM_005199755.3 , XM_024984485.1 , XM_015460159.1 , XM_015469188.1
    Accession Version XM_024984483.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2505bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGCCATCAGT GGCCTATGTA
    CACACCACGC CGGGCCTGCC CTCGGGCTGG GAAGAGAGGA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCTATCAT GCAGCTCGCA
    GAAGACGGCG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CCGCCCCGCT GGAGGGCGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GATACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAGA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA CAACACAAAG
    ACCACGACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ACATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGCTGGG AAGAAAGGAT CCACTTGGAT
    GGCCGGACGT TTTACATTGA CCACAATAGC AAAATTACTC AGTGGGAAGA CCCAAGACTG
    CAGAACCCAG CTATTACTGG TCCGGCTGTT CCTTACTCCA GAGAATTTAA GCAGAAATAT
    GACTACTTTA GAAAGAAATT AAAGAAACCT GCGGATATTC CAAATAGGTT TGAAATGAAA
    CTTCACAGAA ATAACATATT TGAAGAGTCC TATCGGAGAA TCATGTCTGT GAAAAGACCA
    GATGTCCTGA AAGCTAGGCT GTGGATTGAG TTTGAATCGG AGAAAGGTCT GGACTACGGG
    GGCGTGGCCA GAGAGTGGTT CTTCCTACTG TCCAAGGAGA TGTTCAACCC TTACTACGGC
    CTCTTCGAGT ATTCGGCAAC GGACAACTAC ACACTTCAGA TCAATCCCAA TTCAGGCCTC
    TGTAATGAAG ATCATTTGTC CTATTTCACT TTCATTGGAA GAGTTGCTGG GCTGGCAGTA
    TTTCATGGGA AACTTCTAGA TGGTTTCTTC ATTCGACCGT TTTATAAAAT GATGCTGGGA
    AAGCAGATAA CTCTGAATGA CATGGAATCT GTGGATAGCG AATATTACAA CTCTTTGAAA
    TGGATCTTAG AAAATGACCC AACTGAACTG GACCTTATGT TCTGCATTGA TGAAGAGAAC
    TTTGGGCAGA CATATCAAGT GGATCTGAAG CCCAACGGGT CAGAAATAAT GGTAACGAAC
    GAAAACAAGA GAGAGTACAT CGACCTCGTC ATCCAGTGGA GGTTCGTGAA CAGGGTCCAG
    AAGCAGATGA ACGCCTTCCT GGAGGGATTC ACGGAACTGC TCCCTATTGA TTTGATTAAA
    ATCTTTGATG AAAATGAACT GGAGTTGCTG ATGTGTGGCC TTGGCGACGT GGATGTGAAC
    GACTGGCGAC AACATTCTAT TTACAAGAAT GGCTACTGCC CGAACCACCC CGTGATCCAG
    TGGTTCTGGA AGGCTGTGCT GCTGATGGAC GCCGAGAAGC GCATCCGGTT GCTGCAGTTT
    GTCACAGGGA CGTCCCGAGT ACCTATGAAT GGATTTGCCG AGCTTTATGG TTCCAATGGT
    CCTCAGCTGT TTACAATAGA GCAATGGGGA AGTCCTGAAA AACTGCCCAG AGCTCACACG
    TGCTTTAATC GCCTTGACTT ACCTCCGTAT GAAACCTTCG AAGATTTACG AGAGAAGCTC
    CTCATGGCTG TGGAAAACGC CCAAGGCTTC GAGGGGGTGG ATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024840251.1
    CDS420..2924
    Translation

    Target ORF information:

    RefSeq Version XM_024984483.1
    Organism Bos taurus(cattle)
    Definition Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X23, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024984483.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGCCATCAGT GGCCTATGTA
    CACACCACGC CGGGCCTGCC CTCGGGCTGG GAAGAGAGGA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCTATCAT GCAGCTCGCA
    GAAGACGGCG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CCGCCCCGCT GGAGGGCGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GATACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAGA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA CAACACAAAG
    ACCACGACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ACATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGCTGGG AAGAAAGGAT CCACTTGGAT
    GGCCGGACGT TTTACATTGA CCACAATAGC AAAATTACTC AGTGGGAAGA CCCAAGACTG
    CAGAACCCAG CTATTACTGG TCCGGCTGTT CCTTACTCCA GAGAATTTAA GCAGAAATAT
    GACTACTTTA GAAAGAAATT AAAGAAACCT GCGGATATTC CAAATAGGTT TGAAATGAAA
    CTTCACAGAA ATAACATATT TGAAGAGTCC TATCGGAGAA TCATGTCTGT GAAAAGACCA
    GATGTCCTGA AAGCTAGGCT GTGGATTGAG TTTGAATCGG AGAAAGGTCT GGACTACGGG
    GGCGTGGCCA GAGAGTGGTT CTTCCTACTG TCCAAGGAGA TGTTCAACCC TTACTACGGC
    CTCTTCGAGT ATTCGGCAAC GGACAACTAC ACACTTCAGA TCAATCCCAA TTCAGGCCTC
    TGTAATGAAG ATCATTTGTC CTATTTCACT TTCATTGGAA GAGTTGCTGG GCTGGCAGTA
    TTTCATGGGA AACTTCTAGA TGGTTTCTTC ATTCGACCGT TTTATAAAAT GATGCTGGGA
    AAGCAGATAA CTCTGAATGA CATGGAATCT GTGGATAGCG AATATTACAA CTCTTTGAAA
    TGGATCTTAG AAAATGACCC AACTGAACTG GACCTTATGT TCTGCATTGA TGAAGAGAAC
    TTTGGGCAGA CATATCAAGT GGATCTGAAG CCCAACGGGT CAGAAATAAT GGTAACGAAC
    GAAAACAAGA GAGAGTACAT CGACCTCGTC ATCCAGTGGA GGTTCGTGAA CAGGGTCCAG
    AAGCAGATGA ACGCCTTCCT GGAGGGATTC ACGGAACTGC TCCCTATTGA TTTGATTAAA
    ATCTTTGATG AAAATGAACT GGAGTTGCTG ATGTGTGGCC TTGGCGACGT GGATGTGAAC
    GACTGGCGAC AACATTCTAT TTACAAGAAT GGCTACTGCC CGAACCACCC CGTGATCCAG
    TGGTTCTGGA AGGCTGTGCT GCTGATGGAC GCCGAGAAGC GCATCCGGTT GCTGCAGTTT
    GTCACAGGGA CGTCCCGAGT ACCTATGAAT GGATTTGCCG AGCTTTATGG TTCCAATGGT
    CCTCAGCTGT TTACAATAGA GCAATGGGGA AGTCCTGAAA AACTGCCCAG AGCTCACACG
    TGCTTTAATC GCCTTGACTT ACCTCCGTAT GAAACCTTCG AAGATTTACG AGAGAAGCTC
    CTCATGGCTG TGGAAAACGC CCAAGGCTTC GAGGGGGTGG ATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88368
    Clone ID Related Accession (Same CDS sequence) XM_005224353.4 , XM_005224353.3 , XM_024984483.1 , XM_005199755.3 , XM_024984485.1 , XM_015460159.1 , XM_015469188.1
    Accession Version XM_005199755.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2505bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646636.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005199755.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGCCATCAGT GGCCTATGTA
    CACACCACGC CGGGCCTGCC CTCGGGCTGG GAAGAGAGGA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCTATCAT GCAGCTCGCA
    GAAGACGGCG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CCGCCCCGCT GGAGGGCGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GATACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAGA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA CAACACAAAG
    ACCACGACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ACATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGCTGGG AAGAAAGGAT CCACTTGGAT
    GGCCGGACGT TTTACATTGA CCACAATAGC AAAATTACTC AGTGGGAAGA CCCAAGACTG
    CAGAACCCAG CTATTACTGG TCCGGCTGTT CCTTACTCCA GAGAATTTAA GCAGAAATAT
    GACTACTTTA GAAAGAAATT AAAGAAACCT GCGGATATTC CAAATAGGTT TGAAATGAAA
    CTTCACAGAA ATAACATATT TGAAGAGTCC TATCGGAGAA TCATGTCTGT GAAAAGACCA
    GATGTCCTGA AAGCTAGGCT GTGGATTGAG TTTGAATCGG AGAAAGGTCT GGACTACGGG
    GGCGTGGCCA GAGAGTGGTT CTTCCTACTG TCCAAGGAGA TGTTCAACCC TTACTACGGC
    CTCTTCGAGT ATTCGGCAAC GGACAACTAC ACACTTCAGA TCAATCCCAA TTCAGGCCTC
    TGTAATGAAG ATCATTTGTC CTATTTCACT TTCATTGGAA GAGTTGCTGG GCTGGCAGTA
    TTTCATGGGA AACTTCTAGA TGGTTTCTTC ATTCGACCGT TTTATAAAAT GATGCTGGGA
    AAGCAGATAA CTCTGAATGA CATGGAATCT GTGGATAGCG AATATTACAA CTCTTTGAAA
    TGGATCTTAG AAAATGACCC AACTGAACTG GACCTTATGT TCTGCATTGA TGAAGAGAAC
    TTTGGGCAGA CATATCAAGT GGATCTGAAG CCCAACGGGT CAGAAATAAT GGTAACGAAC
    GAAAACAAGA GAGAGTACAT CGACCTCGTC ATCCAGTGGA GGTTCGTGAA CAGGGTCCAG
    AAGCAGATGA ACGCCTTCCT GGAGGGATTC ACGGAACTGC TCCCTATTGA TTTGATTAAA
    ATCTTTGATG AAAATGAACT GGAGTTGCTG ATGTGTGGCC TTGGCGACGT GGATGTGAAC
    GACTGGCGAC AACATTCTAT TTACAAGAAT GGCTACTGCC CGAACCACCC CGTGATCCAG
    TGGTTCTGGA AGGCTGTGCT GCTGATGGAC GCCGAGAAGC GCATCCGGTT GCTGCAGTTT
    GTCACAGGGA CGTCCCGAGT ACCTATGAAT GGATTTGCCG AGCTTTATGG TTCCAATGGT
    CCTCAGCTGT TTACAATAGA GCAATGGGGA AGTCCTGAAA AACTGCCCAG AGCTCACACG
    TGCTTTAATC GCCTTGACTT ACCTCCGTAT GAAACCTTCG AAGATTTACG AGAGAAGCTC
    CTCATGGCTG TGGAAAACGC CCAAGGCTTC GAGGGGGTGG ATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005199812.3
    CDS551..3055
    Translation

    Target ORF information:

    RefSeq Version XM_005199755.3
    Organism Bos taurus(cattle)
    Definition Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005199755.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGCCATCAGT GGCCTATGTA
    CACACCACGC CGGGCCTGCC CTCGGGCTGG GAAGAGAGGA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCTATCAT GCAGCTCGCA
    GAAGACGGCG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CCGCCCCGCT GGAGGGCGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GATACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAGA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA CAACACAAAG
    ACCACGACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ACATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGCTGGG AAGAAAGGAT CCACTTGGAT
    GGCCGGACGT TTTACATTGA CCACAATAGC AAAATTACTC AGTGGGAAGA CCCAAGACTG
    CAGAACCCAG CTATTACTGG TCCGGCTGTT CCTTACTCCA GAGAATTTAA GCAGAAATAT
    GACTACTTTA GAAAGAAATT AAAGAAACCT GCGGATATTC CAAATAGGTT TGAAATGAAA
    CTTCACAGAA ATAACATATT TGAAGAGTCC TATCGGAGAA TCATGTCTGT GAAAAGACCA
    GATGTCCTGA AAGCTAGGCT GTGGATTGAG TTTGAATCGG AGAAAGGTCT GGACTACGGG
    GGCGTGGCCA GAGAGTGGTT CTTCCTACTG TCCAAGGAGA TGTTCAACCC TTACTACGGC
    CTCTTCGAGT ATTCGGCAAC GGACAACTAC ACACTTCAGA TCAATCCCAA TTCAGGCCTC
    TGTAATGAAG ATCATTTGTC CTATTTCACT TTCATTGGAA GAGTTGCTGG GCTGGCAGTA
    TTTCATGGGA AACTTCTAGA TGGTTTCTTC ATTCGACCGT TTTATAAAAT GATGCTGGGA
    AAGCAGATAA CTCTGAATGA CATGGAATCT GTGGATAGCG AATATTACAA CTCTTTGAAA
    TGGATCTTAG AAAATGACCC AACTGAACTG GACCTTATGT TCTGCATTGA TGAAGAGAAC
    TTTGGGCAGA CATATCAAGT GGATCTGAAG CCCAACGGGT CAGAAATAAT GGTAACGAAC
    GAAAACAAGA GAGAGTACAT CGACCTCGTC ATCCAGTGGA GGTTCGTGAA CAGGGTCCAG
    AAGCAGATGA ACGCCTTCCT GGAGGGATTC ACGGAACTGC TCCCTATTGA TTTGATTAAA
    ATCTTTGATG AAAATGAACT GGAGTTGCTG ATGTGTGGCC TTGGCGACGT GGATGTGAAC
    GACTGGCGAC AACATTCTAT TTACAAGAAT GGCTACTGCC CGAACCACCC CGTGATCCAG
    TGGTTCTGGA AGGCTGTGCT GCTGATGGAC GCCGAGAAGC GCATCCGGTT GCTGCAGTTT
    GTCACAGGGA CGTCCCGAGT ACCTATGAAT GGATTTGCCG AGCTTTATGG TTCCAATGGT
    CCTCAGCTGT TTACAATAGA GCAATGGGGA AGTCCTGAAA AACTGCCCAG AGCTCACACG
    TGCTTTAATC GCCTTGACTT ACCTCCGTAT GAAACCTTCG AAGATTTACG AGAGAAGCTC
    CTCATGGCTG TGGAAAACGC CCAAGGCTTC GAGGGGGTGG ATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88368
    Clone ID Related Accession (Same CDS sequence) XM_005224353.4 , XM_005224353.3 , XM_024984483.1 , XM_005199755.3 , XM_024984485.1 , XM_015460159.1 , XM_015469188.1
    Accession Version XM_024984485.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2505bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGCCATCAGT GGCCTATGTA
    CACACCACGC CGGGCCTGCC CTCGGGCTGG GAAGAGAGGA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCTATCAT GCAGCTCGCA
    GAAGACGGCG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CCGCCCCGCT GGAGGGCGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GATACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAGA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA CAACACAAAG
    ACCACGACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ACATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGCTGGG AAGAAAGGAT CCACTTGGAT
    GGCCGGACGT TTTACATTGA CCACAATAGC AAAATTACTC AGTGGGAAGA CCCAAGACTG
    CAGAACCCAG CTATTACTGG TCCGGCTGTT CCTTACTCCA GAGAATTTAA GCAGAAATAT
    GACTACTTTA GAAAGAAATT AAAGAAACCT GCGGATATTC CAAATAGGTT TGAAATGAAA
    CTTCACAGAA ATAACATATT TGAAGAGTCC TATCGGAGAA TCATGTCTGT GAAAAGACCA
    GATGTCCTGA AAGCTAGGCT GTGGATTGAG TTTGAATCGG AGAAAGGTCT GGACTACGGG
    GGCGTGGCCA GAGAGTGGTT CTTCCTACTG TCCAAGGAGA TGTTCAACCC TTACTACGGC
    CTCTTCGAGT ATTCGGCAAC GGACAACTAC ACACTTCAGA TCAATCCCAA TTCAGGCCTC
    TGTAATGAAG ATCATTTGTC CTATTTCACT TTCATTGGAA GAGTTGCTGG GCTGGCAGTA
    TTTCATGGGA AACTTCTAGA TGGTTTCTTC ATTCGACCGT TTTATAAAAT GATGCTGGGA
    AAGCAGATAA CTCTGAATGA CATGGAATCT GTGGATAGCG AATATTACAA CTCTTTGAAA
    TGGATCTTAG AAAATGACCC AACTGAACTG GACCTTATGT TCTGCATTGA TGAAGAGAAC
    TTTGGGCAGA CATATCAAGT GGATCTGAAG CCCAACGGGT CAGAAATAAT GGTAACGAAC
    GAAAACAAGA GAGAGTACAT CGACCTCGTC ATCCAGTGGA GGTTCGTGAA CAGGGTCCAG
    AAGCAGATGA ACGCCTTCCT GGAGGGATTC ACGGAACTGC TCCCTATTGA TTTGATTAAA
    ATCTTTGATG AAAATGAACT GGAGTTGCTG ATGTGTGGCC TTGGCGACGT GGATGTGAAC
    GACTGGCGAC AACATTCTAT TTACAAGAAT GGCTACTGCC CGAACCACCC CGTGATCCAG
    TGGTTCTGGA AGGCTGTGCT GCTGATGGAC GCCGAGAAGC GCATCCGGTT GCTGCAGTTT
    GTCACAGGGA CGTCCCGAGT ACCTATGAAT GGATTTGCCG AGCTTTATGG TTCCAATGGT
    CCTCAGCTGT TTACAATAGA GCAATGGGGA AGTCCTGAAA AACTGCCCAG AGCTCACACG
    TGCTTTAATC GCCTTGACTT ACCTCCGTAT GAAACCTTCG AAGATTTACG AGAGAAGCTC
    CTCATGGCTG TGGAAAACGC CCAAGGCTTC GAGGGGGTGG ATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024840253.1
    CDS270..2774
    Translation

    Target ORF information:

    RefSeq Version XM_024984485.1
    Organism Bos taurus(cattle)
    Definition Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X24, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024984485.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGCCATCAGT GGCCTATGTA
    CACACCACGC CGGGCCTGCC CTCGGGCTGG GAAGAGAGGA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCTATCAT GCAGCTCGCA
    GAAGACGGCG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CCGCCCCGCT GGAGGGCGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GATACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAGA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA CAACACAAAG
    ACCACGACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ACATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGCTGGG AAGAAAGGAT CCACTTGGAT
    GGCCGGACGT TTTACATTGA CCACAATAGC AAAATTACTC AGTGGGAAGA CCCAAGACTG
    CAGAACCCAG CTATTACTGG TCCGGCTGTT CCTTACTCCA GAGAATTTAA GCAGAAATAT
    GACTACTTTA GAAAGAAATT AAAGAAACCT GCGGATATTC CAAATAGGTT TGAAATGAAA
    CTTCACAGAA ATAACATATT TGAAGAGTCC TATCGGAGAA TCATGTCTGT GAAAAGACCA
    GATGTCCTGA AAGCTAGGCT GTGGATTGAG TTTGAATCGG AGAAAGGTCT GGACTACGGG
    GGCGTGGCCA GAGAGTGGTT CTTCCTACTG TCCAAGGAGA TGTTCAACCC TTACTACGGC
    CTCTTCGAGT ATTCGGCAAC GGACAACTAC ACACTTCAGA TCAATCCCAA TTCAGGCCTC
    TGTAATGAAG ATCATTTGTC CTATTTCACT TTCATTGGAA GAGTTGCTGG GCTGGCAGTA
    TTTCATGGGA AACTTCTAGA TGGTTTCTTC ATTCGACCGT TTTATAAAAT GATGCTGGGA
    AAGCAGATAA CTCTGAATGA CATGGAATCT GTGGATAGCG AATATTACAA CTCTTTGAAA
    TGGATCTTAG AAAATGACCC AACTGAACTG GACCTTATGT TCTGCATTGA TGAAGAGAAC
    TTTGGGCAGA CATATCAAGT GGATCTGAAG CCCAACGGGT CAGAAATAAT GGTAACGAAC
    GAAAACAAGA GAGAGTACAT CGACCTCGTC ATCCAGTGGA GGTTCGTGAA CAGGGTCCAG
    AAGCAGATGA ACGCCTTCCT GGAGGGATTC ACGGAACTGC TCCCTATTGA TTTGATTAAA
    ATCTTTGATG AAAATGAACT GGAGTTGCTG ATGTGTGGCC TTGGCGACGT GGATGTGAAC
    GACTGGCGAC AACATTCTAT TTACAAGAAT GGCTACTGCC CGAACCACCC CGTGATCCAG
    TGGTTCTGGA AGGCTGTGCT GCTGATGGAC GCCGAGAAGC GCATCCGGTT GCTGCAGTTT
    GTCACAGGGA CGTCCCGAGT ACCTATGAAT GGATTTGCCG AGCTTTATGG TTCCAATGGT
    CCTCAGCTGT TTACAATAGA GCAATGGGGA AGTCCTGAAA AACTGCCCAG AGCTCACACG
    TGCTTTAATC GCCTTGACTT ACCTCCGTAT GAAACCTTCG AAGATTTACG AGAGAAGCTC
    CTCATGGCTG TGGAAAACGC CCAAGGCTTC GAGGGGGTGG ATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88368
    Clone ID Related Accession (Same CDS sequence) XM_005224353.4 , XM_005224353.3 , XM_024984483.1 , XM_005199755.3 , XM_024984485.1 , XM_015460159.1 , XM_015469188.1
    Accession Version XM_015460159.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2505bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104569.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGCCATCAGT GGCCTATGTA
    CACACCACGC CGGGCCTGCC CTCGGGCTGG GAAGAGAGGA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCTATCAT GCAGCTCGCA
    GAAGACGGCG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CCGCCCCGCT GGAGGGCGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GATACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAGA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA CAACACAAAG
    ACCACGACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ACATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGCTGGG AAGAAAGGAT CCACTTGGAT
    GGCCGGACGT TTTACATTGA CCACAATAGC AAAATTACTC AGTGGGAAGA CCCAAGACTG
    CAGAACCCAG CTATTACTGG TCCGGCTGTT CCTTACTCCA GAGAATTTAA GCAGAAATAT
    GACTACTTTA GAAAGAAATT AAAGAAACCT GCGGATATTC CAAATAGGTT TGAAATGAAA
    CTTCACAGAA ATAACATATT TGAAGAGTCC TATCGGAGAA TCATGTCTGT GAAAAGACCA
    GATGTCCTGA AAGCTAGGCT GTGGATTGAG TTTGAATCGG AGAAAGGTCT GGACTACGGG
    GGCGTGGCCA GAGAGTGGTT CTTCCTACTG TCCAAGGAGA TGTTCAACCC TTACTACGGC
    CTCTTCGAGT ATTCGGCAAC GGACAACTAC ACACTTCAGA TCAATCCCAA TTCAGGCCTC
    TGTAATGAAG ATCATTTGTC CTATTTCACT TTCATTGGAA GAGTTGCTGG GCTGGCAGTA
    TTTCATGGGA AACTTCTAGA TGGTTTCTTC ATTCGACCGT TTTATAAAAT GATGCTGGGA
    AAGCAGATAA CTCTGAATGA CATGGAATCT GTGGATAGCG AATATTACAA CTCTTTGAAA
    TGGATCTTAG AAAATGACCC AACTGAACTG GACCTTATGT TCTGCATTGA TGAAGAGAAC
    TTTGGGCAGA CATATCAAGT GGATCTGAAG CCCAACGGGT CAGAAATAAT GGTAACGAAC
    GAAAACAAGA GAGAGTACAT CGACCTCGTC ATCCAGTGGA GGTTCGTGAA CAGGGTCCAG
    AAGCAGATGA ACGCCTTCCT GGAGGGATTC ACGGAACTGC TCCCTATTGA TTTGATTAAA
    ATCTTTGATG AAAATGAACT GGAGTTGCTG ATGTGTGGCC TTGGCGACGT GGATGTGAAC
    GACTGGCGAC AACATTCTAT TTACAAGAAT GGCTACTGCC CGAACCACCC CGTGATCCAG
    TGGTTCTGGA AGGCTGTGCT GCTGATGGAC GCCGAGAAGC GCATCCGGTT GCTGCAGTTT
    GTCACAGGGA CGTCCCGAGT ACCTATGAAT GGATTTGCCG AGCTTTATGG TTCCAATGGT
    CCTCAGCTGT TTACAATAGA GCAATGGGGA AGTCCTGAAA AACTGCCCAG AGCTCACACG
    TGCTTTAATC GCCTTGACTT ACCTCCGTAT GAAACCTTCG AAGATTTACG AGAGAAGCTC
    CTCATGGCTG TGGAAAACGC CCAAGGCTTC GAGGGGGTGG ATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015315645.1
    CDS316..2820
    Translation

    Target ORF information:

    RefSeq Version XM_015460159.1
    Organism Bos taurus(cattle)
    Definition Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015460159.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGCCATCAGT GGCCTATGTA
    CACACCACGC CGGGCCTGCC CTCGGGCTGG GAAGAGAGGA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCTATCAT GCAGCTCGCA
    GAAGACGGCG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CCGCCCCGCT GGAGGGCGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GATACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAGA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA CAACACAAAG
    ACCACGACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ACATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGCTGGG AAGAAAGGAT CCACTTGGAT
    GGCCGGACGT TTTACATTGA CCACAATAGC AAAATTACTC AGTGGGAAGA CCCAAGACTG
    CAGAACCCAG CTATTACTGG TCCGGCTGTT CCTTACTCCA GAGAATTTAA GCAGAAATAT
    GACTACTTTA GAAAGAAATT AAAGAAACCT GCGGATATTC CAAATAGGTT TGAAATGAAA
    CTTCACAGAA ATAACATATT TGAAGAGTCC TATCGGAGAA TCATGTCTGT GAAAAGACCA
    GATGTCCTGA AAGCTAGGCT GTGGATTGAG TTTGAATCGG AGAAAGGTCT GGACTACGGG
    GGCGTGGCCA GAGAGTGGTT CTTCCTACTG TCCAAGGAGA TGTTCAACCC TTACTACGGC
    CTCTTCGAGT ATTCGGCAAC GGACAACTAC ACACTTCAGA TCAATCCCAA TTCAGGCCTC
    TGTAATGAAG ATCATTTGTC CTATTTCACT TTCATTGGAA GAGTTGCTGG GCTGGCAGTA
    TTTCATGGGA AACTTCTAGA TGGTTTCTTC ATTCGACCGT TTTATAAAAT GATGCTGGGA
    AAGCAGATAA CTCTGAATGA CATGGAATCT GTGGATAGCG AATATTACAA CTCTTTGAAA
    TGGATCTTAG AAAATGACCC AACTGAACTG GACCTTATGT TCTGCATTGA TGAAGAGAAC
    TTTGGGCAGA CATATCAAGT GGATCTGAAG CCCAACGGGT CAGAAATAAT GGTAACGAAC
    GAAAACAAGA GAGAGTACAT CGACCTCGTC ATCCAGTGGA GGTTCGTGAA CAGGGTCCAG
    AAGCAGATGA ACGCCTTCCT GGAGGGATTC ACGGAACTGC TCCCTATTGA TTTGATTAAA
    ATCTTTGATG AAAATGAACT GGAGTTGCTG ATGTGTGGCC TTGGCGACGT GGATGTGAAC
    GACTGGCGAC AACATTCTAT TTACAAGAAT GGCTACTGCC CGAACCACCC CGTGATCCAG
    TGGTTCTGGA AGGCTGTGCT GCTGATGGAC GCCGAGAAGC GCATCCGGTT GCTGCAGTTT
    GTCACAGGGA CGTCCCGAGT ACCTATGAAT GGATTTGCCG AGCTTTATGG TTCCAATGGT
    CCTCAGCTGT TTACAATAGA GCAATGGGGA AGTCCTGAAA AACTGCCCAG AGCTCACACG
    TGCTTTAATC GCCTTGACTT ACCTCCGTAT GAAACCTTCG AAGATTTACG AGAGAAGCTC
    CTCATGGCTG TGGAAAACGC CCAAGGCTTC GAGGGGGTGG ATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88368
    Clone ID Related Accession (Same CDS sequence) XM_005224353.4 , XM_005224353.3 , XM_024984483.1 , XM_005199755.3 , XM_024984485.1 , XM_015460159.1 , XM_015469188.1
    Accession Version XM_015469188.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2505bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646636.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGCCATCAGT GGCCTATGTA
    CACACCACGC CGGGCCTGCC CTCGGGCTGG GAAGAGAGGA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCTATCAT GCAGCTCGCA
    GAAGACGGCG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CCGCCCCGCT GGAGGGCGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GATACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAGA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA CAACACAAAG
    ACCACGACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ACATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGCTGGG AAGAAAGGAT CCACTTGGAT
    GGCCGGACGT TTTACATTGA CCACAATAGC AAAATTACTC AGTGGGAAGA CCCAAGACTG
    CAGAACCCAG CTATTACTGG TCCGGCTGTT CCTTACTCCA GAGAATTTAA GCAGAAATAT
    GACTACTTTA GAAAGAAATT AAAGAAACCT GCGGATATTC CAAATAGGTT TGAAATGAAA
    CTTCACAGAA ATAACATATT TGAAGAGTCC TATCGGAGAA TCATGTCTGT GAAAAGACCA
    GATGTCCTGA AAGCTAGGCT GTGGATTGAG TTTGAATCGG AGAAAGGTCT GGACTACGGG
    GGCGTGGCCA GAGAGTGGTT CTTCCTACTG TCCAAGGAGA TGTTCAACCC TTACTACGGC
    CTCTTCGAGT ATTCGGCAAC GGACAACTAC ACACTTCAGA TCAATCCCAA TTCAGGCCTC
    TGTAATGAAG ATCATTTGTC CTATTTCACT TTCATTGGAA GAGTTGCTGG GCTGGCAGTA
    TTTCATGGGA AACTTCTAGA TGGTTTCTTC ATTCGACCGT TTTATAAAAT GATGCTGGGA
    AAGCAGATAA CTCTGAATGA CATGGAATCT GTGGATAGCG AATATTACAA CTCTTTGAAA
    TGGATCTTAG AAAATGACCC AACTGAACTG GACCTTATGT TCTGCATTGA TGAAGAGAAC
    TTTGGGCAGA CATATCAAGT GGATCTGAAG CCCAACGGGT CAGAAATAAT GGTAACGAAC
    GAAAACAAGA GAGAGTACAT CGACCTCGTC ATCCAGTGGA GGTTCGTGAA CAGGGTCCAG
    AAGCAGATGA ACGCCTTCCT GGAGGGATTC ACGGAACTGC TCCCTATTGA TTTGATTAAA
    ATCTTTGATG AAAATGAACT GGAGTTGCTG ATGTGTGGCC TTGGCGACGT GGATGTGAAC
    GACTGGCGAC AACATTCTAT TTACAAGAAT GGCTACTGCC CGAACCACCC CGTGATCCAG
    TGGTTCTGGA AGGCTGTGCT GCTGATGGAC GCCGAGAAGC GCATCCGGTT GCTGCAGTTT
    GTCACAGGGA CGTCCCGAGT ACCTATGAAT GGATTTGCCG AGCTTTATGG TTCCAATGGT
    CCTCAGCTGT TTACAATAGA GCAATGGGGA AGTCCTGAAA AACTGCCCAG AGCTCACACG
    TGCTTTAATC GCCTTGACTT ACCTCCGTAT GAAACCTTCG AAGATTTACG AGAGAAGCTC
    CTCATGGCTG TGGAAAACGC CCAAGGCTTC GAGGGGGTGG ATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015324674.1
    CDS316..2820
    Translation

    Target ORF information:

    RefSeq Version XM_015469188.1
    Organism Bos taurus(cattle)
    Definition Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015469188.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGCCATCAGT GGCCTATGTA
    CACACCACGC CGGGCCTGCC CTCGGGCTGG GAAGAGAGGA AAGATGCTAA GGGACGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCTATCAT GCAGCTCGCA
    GAAGACGGCG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CCGCCCCGCT GGAGGGCGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GATACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC
    GGCTGGGAGA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA CAACACAAAG
    ACCACGACGT GGGAAGATCC ACGCCTGAAA TTTCCAGTAC ACATGCGGTC AAAGGCATCC
    TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGCTGGG AAGAAAGGAT CCACTTGGAT
    GGCCGGACGT TTTACATTGA CCACAATAGC AAAATTACTC AGTGGGAAGA CCCAAGACTG
    CAGAACCCAG CTATTACTGG TCCGGCTGTT CCTTACTCCA GAGAATTTAA GCAGAAATAT
    GACTACTTTA GAAAGAAATT AAAGAAACCT GCGGATATTC CAAATAGGTT TGAAATGAAA
    CTTCACAGAA ATAACATATT TGAAGAGTCC TATCGGAGAA TCATGTCTGT GAAAAGACCA
    GATGTCCTGA AAGCTAGGCT GTGGATTGAG TTTGAATCGG AGAAAGGTCT GGACTACGGG
    GGCGTGGCCA GAGAGTGGTT CTTCCTACTG TCCAAGGAGA TGTTCAACCC TTACTACGGC
    CTCTTCGAGT ATTCGGCAAC GGACAACTAC ACACTTCAGA TCAATCCCAA TTCAGGCCTC
    TGTAATGAAG ATCATTTGTC CTATTTCACT TTCATTGGAA GAGTTGCTGG GCTGGCAGTA
    TTTCATGGGA AACTTCTAGA TGGTTTCTTC ATTCGACCGT TTTATAAAAT GATGCTGGGA
    AAGCAGATAA CTCTGAATGA CATGGAATCT GTGGATAGCG AATATTACAA CTCTTTGAAA
    TGGATCTTAG AAAATGACCC AACTGAACTG GACCTTATGT TCTGCATTGA TGAAGAGAAC
    TTTGGGCAGA CATATCAAGT GGATCTGAAG CCCAACGGGT CAGAAATAAT GGTAACGAAC
    GAAAACAAGA GAGAGTACAT CGACCTCGTC ATCCAGTGGA GGTTCGTGAA CAGGGTCCAG
    AAGCAGATGA ACGCCTTCCT GGAGGGATTC ACGGAACTGC TCCCTATTGA TTTGATTAAA
    ATCTTTGATG AAAATGAACT GGAGTTGCTG ATGTGTGGCC TTGGCGACGT GGATGTGAAC
    GACTGGCGAC AACATTCTAT TTACAAGAAT GGCTACTGCC CGAACCACCC CGTGATCCAG
    TGGTTCTGGA AGGCTGTGCT GCTGATGGAC GCCGAGAAGC GCATCCGGTT GCTGCAGTTT
    GTCACAGGGA CGTCCCGAGT ACCTATGAAT GGATTTGCCG AGCTTTATGG TTCCAATGGT
    CCTCAGCTGT TTACAATAGA GCAATGGGGA AGTCCTGAAA AACTGCCCAG AGCTCACACG
    TGCTTTAATC GCCTTGACTT ACCTCCGTAT GAAACCTTCG AAGATTTACG AGAGAAGCTC
    CTCATGGCTG TGGAAAACGC CCAAGGCTTC GAGGGGGTGG ATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88369
    Clone ID Related Accession (Same CDS sequence) XM_005224354.3 , XM_005199756.3 , XM_005224354.4
    Accession Version XM_005224354.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2307bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104569.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005224354.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGGGCGCCAA GGACTCACCC
    GTACGTCGGG CTGTGAAAGA TACCCTTTCC AACCCACAGT CCCCACAGCC ATCACCTTAC
    AACTCCCCCA AACCACAACA CAAAGTCACA CAGAGCTTCC TGCCGCCCGG CTGGGAGATG
    AGGATAGCGC CCAACGGCCG GCCCTTCTTC ATTGACCACA ACACAAAGAC CACGACGTGG
    GAAGATCCAC GCCTGAAATT TCCAGTACAC ATGCGGTCAA AGGCATCCTT AAACCCCAAT
    GACCTTGGCC CTCTTCCTCC TGGCTGGGAA GAAAGGATCC ACTTGGATGG CCGGACGTTT
    TACATTGACC ACAGCAGCCA GGTGTTATGT GGCGAGGACG ATGCCAGAGA CTCAGTGCCC
    TCGTACGATA GCAAAATTAC TCAGTGGGAA GACCCAAGAC TGCAGAACCC AGCTATTACT
    GGTCCGGCTG TTCCTTACTC CAGAGAATTT AAGCAGAAAT ATGACTACTT TAGAAAGAAA
    TTAAAGAAAC CTGCGGATAT TCCAAATAGG TTTGAAATGA AACTTCACAG AAATAACATA
    TTTGAAGAGT CCTATCGGAG AATCATGTCT GTGAAAAGAC CAGATGTCCT GAAAGCTAGG
    CTGTGGATTG AGTTTGAATC GGAGAAAGGT CTGGACTACG GGGGCGTGGC CAGAGAGTGG
    TTCTTCCTAC TGTCCAAGGA GATGTTCAAC CCTTACTACG GCCTCTTCGA GTATTCGGCA
    ACGGACAACT ACACACTTCA GATCAATCCC AATTCAGGCC TCTGTAATGA AGATCATTTG
    TCCTATTTCA CTTTCATTGG AAGAGTTGCT GGGCTGGCAG TATTTCATGG GAAACTTCTA
    GATGGTTTCT TCATTCGACC GTTTTATAAA ATGATGCTGG GAAAGCAGAT AACTCTGAAT
    GACATGGAAT CTGTGGATAG CGAATATTAC AACTCTTTGA AATGGATCTT AGAAAATGAC
    CCAACTGAAC TGGACCTTAT GTTCTGCATT GATGAAGAGA ACTTTGGGCA GACATATCAA
    GTGGATCTGA AGCCCAACGG GTCAGAAATA ATGGTAACGA ACGAAAACAA GAGAGAGTAC
    ATCGACCTCG TCATCCAGTG GAGGTTCGTG AACAGGGTCC AGAAGCAGAT GAACGCCTTC
    CTGGAGGGAT TCACGGAACT GCTCCCTATT GATTTGATTA AAATCTTTGA TGAAAATGAA
    CTGGAGTTGC TGATGTGTGG CCTTGGCGAC GTGGATGTGA ACGACTGGCG ACAACATTCT
    ATTTACAAGA ATGGCTACTG CCCGAACCAC CCCGTGATCC AGTGGTTCTG GAAGGCTGTG
    CTGCTGATGG ACGCCGAGAA GCGCATCCGG TTGCTGCAGT TTGTCACAGG GACGTCCCGA
    GTACCTATGA ATGGATTTGC CGAGCTTTAT GGTTCCAATG GTCCTCAGCT GTTTACAATA
    GAGCAATGGG GAAGTCCTGA AAAACTGCCC AGAGCTCACA CGTGCTTTAA TCGCCTTGAC
    TTACCTCCGT ATGAAACCTT CGAAGATTTA CGAGAGAAGC TCCTCATGGC TGTGGAAAAC
    GCCCAAGGCT TCGAGGGGGT GGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005224411.3
    CDS563..2869
    Translation

    Target ORF information:

    RefSeq Version XM_005224354.3
    Organism Bos taurus(cattle)
    Definition Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005224354.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGGGCGCCAA GGACTCACCC
    GTACGTCGGG CTGTGAAAGA TACCCTTTCC AACCCACAGT CCCCACAGCC ATCACCTTAC
    AACTCCCCCA AACCACAACA CAAAGTCACA CAGAGCTTCC TGCCGCCCGG CTGGGAGATG
    AGGATAGCGC CCAACGGCCG GCCCTTCTTC ATTGACCACA ACACAAAGAC CACGACGTGG
    GAAGATCCAC GCCTGAAATT TCCAGTACAC ATGCGGTCAA AGGCATCCTT AAACCCCAAT
    GACCTTGGCC CTCTTCCTCC TGGCTGGGAA GAAAGGATCC ACTTGGATGG CCGGACGTTT
    TACATTGACC ACAGCAGCCA GGTGTTATGT GGCGAGGACG ATGCCAGAGA CTCAGTGCCC
    TCGTACGATA GCAAAATTAC TCAGTGGGAA GACCCAAGAC TGCAGAACCC AGCTATTACT
    GGTCCGGCTG TTCCTTACTC CAGAGAATTT AAGCAGAAAT ATGACTACTT TAGAAAGAAA
    TTAAAGAAAC CTGCGGATAT TCCAAATAGG TTTGAAATGA AACTTCACAG AAATAACATA
    TTTGAAGAGT CCTATCGGAG AATCATGTCT GTGAAAAGAC CAGATGTCCT GAAAGCTAGG
    CTGTGGATTG AGTTTGAATC GGAGAAAGGT CTGGACTACG GGGGCGTGGC CAGAGAGTGG
    TTCTTCCTAC TGTCCAAGGA GATGTTCAAC CCTTACTACG GCCTCTTCGA GTATTCGGCA
    ACGGACAACT ACACACTTCA GATCAATCCC AATTCAGGCC TCTGTAATGA AGATCATTTG
    TCCTATTTCA CTTTCATTGG AAGAGTTGCT GGGCTGGCAG TATTTCATGG GAAACTTCTA
    GATGGTTTCT TCATTCGACC GTTTTATAAA ATGATGCTGG GAAAGCAGAT AACTCTGAAT
    GACATGGAAT CTGTGGATAG CGAATATTAC AACTCTTTGA AATGGATCTT AGAAAATGAC
    CCAACTGAAC TGGACCTTAT GTTCTGCATT GATGAAGAGA ACTTTGGGCA GACATATCAA
    GTGGATCTGA AGCCCAACGG GTCAGAAATA ATGGTAACGA ACGAAAACAA GAGAGAGTAC
    ATCGACCTCG TCATCCAGTG GAGGTTCGTG AACAGGGTCC AGAAGCAGAT GAACGCCTTC
    CTGGAGGGAT TCACGGAACT GCTCCCTATT GATTTGATTA AAATCTTTGA TGAAAATGAA
    CTGGAGTTGC TGATGTGTGG CCTTGGCGAC GTGGATGTGA ACGACTGGCG ACAACATTCT
    ATTTACAAGA ATGGCTACTG CCCGAACCAC CCCGTGATCC AGTGGTTCTG GAAGGCTGTG
    CTGCTGATGG ACGCCGAGAA GCGCATCCGG TTGCTGCAGT TTGTCACAGG GACGTCCCGA
    GTACCTATGA ATGGATTTGC CGAGCTTTAT GGTTCCAATG GTCCTCAGCT GTTTACAATA
    GAGCAATGGG GAAGTCCTGA AAAACTGCCC AGAGCTCACA CGTGCTTTAA TCGCCTTGAC
    TTACCTCCGT ATGAAACCTT CGAAGATTTA CGAGAGAAGC TCCTCATGGC TGTGGAAAAC
    GCCCAAGGCT TCGAGGGGGT GGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88369
    Clone ID Related Accession (Same CDS sequence) XM_005224354.3 , XM_005199756.3 , XM_005224354.4
    Accession Version XM_005199756.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2307bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646636.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005199756.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGGGCGCCAA GGACTCACCC
    GTACGTCGGG CTGTGAAAGA TACCCTTTCC AACCCACAGT CCCCACAGCC ATCACCTTAC
    AACTCCCCCA AACCACAACA CAAAGTCACA CAGAGCTTCC TGCCGCCCGG CTGGGAGATG
    AGGATAGCGC CCAACGGCCG GCCCTTCTTC ATTGACCACA ACACAAAGAC CACGACGTGG
    GAAGATCCAC GCCTGAAATT TCCAGTACAC ATGCGGTCAA AGGCATCCTT AAACCCCAAT
    GACCTTGGCC CTCTTCCTCC TGGCTGGGAA GAAAGGATCC ACTTGGATGG CCGGACGTTT
    TACATTGACC ACAGCAGCCA GGTGTTATGT GGCGAGGACG ATGCCAGAGA CTCAGTGCCC
    TCGTACGATA GCAAAATTAC TCAGTGGGAA GACCCAAGAC TGCAGAACCC AGCTATTACT
    GGTCCGGCTG TTCCTTACTC CAGAGAATTT AAGCAGAAAT ATGACTACTT TAGAAAGAAA
    TTAAAGAAAC CTGCGGATAT TCCAAATAGG TTTGAAATGA AACTTCACAG AAATAACATA
    TTTGAAGAGT CCTATCGGAG AATCATGTCT GTGAAAAGAC CAGATGTCCT GAAAGCTAGG
    CTGTGGATTG AGTTTGAATC GGAGAAAGGT CTGGACTACG GGGGCGTGGC CAGAGAGTGG
    TTCTTCCTAC TGTCCAAGGA GATGTTCAAC CCTTACTACG GCCTCTTCGA GTATTCGGCA
    ACGGACAACT ACACACTTCA GATCAATCCC AATTCAGGCC TCTGTAATGA AGATCATTTG
    TCCTATTTCA CTTTCATTGG AAGAGTTGCT GGGCTGGCAG TATTTCATGG GAAACTTCTA
    GATGGTTTCT TCATTCGACC GTTTTATAAA ATGATGCTGG GAAAGCAGAT AACTCTGAAT
    GACATGGAAT CTGTGGATAG CGAATATTAC AACTCTTTGA AATGGATCTT AGAAAATGAC
    CCAACTGAAC TGGACCTTAT GTTCTGCATT GATGAAGAGA ACTTTGGGCA GACATATCAA
    GTGGATCTGA AGCCCAACGG GTCAGAAATA ATGGTAACGA ACGAAAACAA GAGAGAGTAC
    ATCGACCTCG TCATCCAGTG GAGGTTCGTG AACAGGGTCC AGAAGCAGAT GAACGCCTTC
    CTGGAGGGAT TCACGGAACT GCTCCCTATT GATTTGATTA AAATCTTTGA TGAAAATGAA
    CTGGAGTTGC TGATGTGTGG CCTTGGCGAC GTGGATGTGA ACGACTGGCG ACAACATTCT
    ATTTACAAGA ATGGCTACTG CCCGAACCAC CCCGTGATCC AGTGGTTCTG GAAGGCTGTG
    CTGCTGATGG ACGCCGAGAA GCGCATCCGG TTGCTGCAGT TTGTCACAGG GACGTCCCGA
    GTACCTATGA ATGGATTTGC CGAGCTTTAT GGTTCCAATG GTCCTCAGCT GTTTACAATA
    GAGCAATGGG GAAGTCCTGA AAAACTGCCC AGAGCTCACA CGTGCTTTAA TCGCCTTGAC
    TTACCTCCGT ATGAAACCTT CGAAGATTTA CGAGAGAAGC TCCTCATGGC TGTGGAAAAC
    GCCCAAGGCT TCGAGGGGGT GGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005199813.3
    CDS563..2869
    Translation

    Target ORF information:

    RefSeq Version XM_005199756.3
    Organism Bos taurus(cattle)
    Definition Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005199756.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGGGCGCCAA GGACTCACCC
    GTACGTCGGG CTGTGAAAGA TACCCTTTCC AACCCACAGT CCCCACAGCC ATCACCTTAC
    AACTCCCCCA AACCACAACA CAAAGTCACA CAGAGCTTCC TGCCGCCCGG CTGGGAGATG
    AGGATAGCGC CCAACGGCCG GCCCTTCTTC ATTGACCACA ACACAAAGAC CACGACGTGG
    GAAGATCCAC GCCTGAAATT TCCAGTACAC ATGCGGTCAA AGGCATCCTT AAACCCCAAT
    GACCTTGGCC CTCTTCCTCC TGGCTGGGAA GAAAGGATCC ACTTGGATGG CCGGACGTTT
    TACATTGACC ACAGCAGCCA GGTGTTATGT GGCGAGGACG ATGCCAGAGA CTCAGTGCCC
    TCGTACGATA GCAAAATTAC TCAGTGGGAA GACCCAAGAC TGCAGAACCC AGCTATTACT
    GGTCCGGCTG TTCCTTACTC CAGAGAATTT AAGCAGAAAT ATGACTACTT TAGAAAGAAA
    TTAAAGAAAC CTGCGGATAT TCCAAATAGG TTTGAAATGA AACTTCACAG AAATAACATA
    TTTGAAGAGT CCTATCGGAG AATCATGTCT GTGAAAAGAC CAGATGTCCT GAAAGCTAGG
    CTGTGGATTG AGTTTGAATC GGAGAAAGGT CTGGACTACG GGGGCGTGGC CAGAGAGTGG
    TTCTTCCTAC TGTCCAAGGA GATGTTCAAC CCTTACTACG GCCTCTTCGA GTATTCGGCA
    ACGGACAACT ACACACTTCA GATCAATCCC AATTCAGGCC TCTGTAATGA AGATCATTTG
    TCCTATTTCA CTTTCATTGG AAGAGTTGCT GGGCTGGCAG TATTTCATGG GAAACTTCTA
    GATGGTTTCT TCATTCGACC GTTTTATAAA ATGATGCTGG GAAAGCAGAT AACTCTGAAT
    GACATGGAAT CTGTGGATAG CGAATATTAC AACTCTTTGA AATGGATCTT AGAAAATGAC
    CCAACTGAAC TGGACCTTAT GTTCTGCATT GATGAAGAGA ACTTTGGGCA GACATATCAA
    GTGGATCTGA AGCCCAACGG GTCAGAAATA ATGGTAACGA ACGAAAACAA GAGAGAGTAC
    ATCGACCTCG TCATCCAGTG GAGGTTCGTG AACAGGGTCC AGAAGCAGAT GAACGCCTTC
    CTGGAGGGAT TCACGGAACT GCTCCCTATT GATTTGATTA AAATCTTTGA TGAAAATGAA
    CTGGAGTTGC TGATGTGTGG CCTTGGCGAC GTGGATGTGA ACGACTGGCG ACAACATTCT
    ATTTACAAGA ATGGCTACTG CCCGAACCAC CCCGTGATCC AGTGGTTCTG GAAGGCTGTG
    CTGCTGATGG ACGCCGAGAA GCGCATCCGG TTGCTGCAGT TTGTCACAGG GACGTCCCGA
    GTACCTATGA ATGGATTTGC CGAGCTTTAT GGTTCCAATG GTCCTCAGCT GTTTACAATA
    GAGCAATGGG GAAGTCCTGA AAAACTGCCC AGAGCTCACA CGTGCTTTAA TCGCCTTGAC
    TTACCTCCGT ATGAAACCTT CGAAGATTTA CGAGAGAAGC TCCTCATGGC TGTGGAAAAC
    GCCCAAGGCT TCGAGGGGGT GGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88369
    Clone ID Related Accession (Same CDS sequence) XM_005224354.3 , XM_005199756.3 , XM_005224354.4
    Accession Version XM_005224354.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2307bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005224354.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGGGCGCCAA GGACTCACCC
    GTACGTCGGG CTGTGAAAGA TACCCTTTCC AACCCACAGT CCCCACAGCC ATCACCTTAC
    AACTCCCCCA AACCACAACA CAAAGTCACA CAGAGCTTCC TGCCGCCCGG CTGGGAGATG
    AGGATAGCGC CCAACGGCCG GCCCTTCTTC ATTGACCACA ACACAAAGAC CACGACGTGG
    GAAGATCCAC GCCTGAAATT TCCAGTACAC ATGCGGTCAA AGGCATCCTT AAACCCCAAT
    GACCTTGGCC CTCTTCCTCC TGGCTGGGAA GAAAGGATCC ACTTGGATGG CCGGACGTTT
    TACATTGACC ACAGCAGCCA GGTGTTATGT GGCGAGGACG ATGCCAGAGA CTCAGTGCCC
    TCGTACGATA GCAAAATTAC TCAGTGGGAA GACCCAAGAC TGCAGAACCC AGCTATTACT
    GGTCCGGCTG TTCCTTACTC CAGAGAATTT AAGCAGAAAT ATGACTACTT TAGAAAGAAA
    TTAAAGAAAC CTGCGGATAT TCCAAATAGG TTTGAAATGA AACTTCACAG AAATAACATA
    TTTGAAGAGT CCTATCGGAG AATCATGTCT GTGAAAAGAC CAGATGTCCT GAAAGCTAGG
    CTGTGGATTG AGTTTGAATC GGAGAAAGGT CTGGACTACG GGGGCGTGGC CAGAGAGTGG
    TTCTTCCTAC TGTCCAAGGA GATGTTCAAC CCTTACTACG GCCTCTTCGA GTATTCGGCA
    ACGGACAACT ACACACTTCA GATCAATCCC AATTCAGGCC TCTGTAATGA AGATCATTTG
    TCCTATTTCA CTTTCATTGG AAGAGTTGCT GGGCTGGCAG TATTTCATGG GAAACTTCTA
    GATGGTTTCT TCATTCGACC GTTTTATAAA ATGATGCTGG GAAAGCAGAT AACTCTGAAT
    GACATGGAAT CTGTGGATAG CGAATATTAC AACTCTTTGA AATGGATCTT AGAAAATGAC
    CCAACTGAAC TGGACCTTAT GTTCTGCATT GATGAAGAGA ACTTTGGGCA GACATATCAA
    GTGGATCTGA AGCCCAACGG GTCAGAAATA ATGGTAACGA ACGAAAACAA GAGAGAGTAC
    ATCGACCTCG TCATCCAGTG GAGGTTCGTG AACAGGGTCC AGAAGCAGAT GAACGCCTTC
    CTGGAGGGAT TCACGGAACT GCTCCCTATT GATTTGATTA AAATCTTTGA TGAAAATGAA
    CTGGAGTTGC TGATGTGTGG CCTTGGCGAC GTGGATGTGA ACGACTGGCG ACAACATTCT
    ATTTACAAGA ATGGCTACTG CCCGAACCAC CCCGTGATCC AGTGGTTCTG GAAGGCTGTG
    CTGCTGATGG ACGCCGAGAA GCGCATCCGG TTGCTGCAGT TTGTCACAGG GACGTCCCGA
    GTACCTATGA ATGGATTTGC CGAGCTTTAT GGTTCCAATG GTCCTCAGCT GTTTACAATA
    GAGCAATGGG GAAGTCCTGA AAAACTGCCC AGAGCTCACA CGTGCTTTAA TCGCCTTGAC
    TTACCTCCGT ATGAAACCTT CGAAGATTTA CGAGAGAAGC TCCTCATGGC TGTGGAAAAC
    GCCCAAGGCT TCGAGGGGGT GGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005224411.3
    CDS566..2872
    Translation

    Target ORF information:

    RefSeq Version XM_005224354.4
    Organism Bos taurus(cattle)
    Definition Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005224354.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGGGCGCCAA GGACTCACCC
    GTACGTCGGG CTGTGAAAGA TACCCTTTCC AACCCACAGT CCCCACAGCC ATCACCTTAC
    AACTCCCCCA AACCACAACA CAAAGTCACA CAGAGCTTCC TGCCGCCCGG CTGGGAGATG
    AGGATAGCGC CCAACGGCCG GCCCTTCTTC ATTGACCACA ACACAAAGAC CACGACGTGG
    GAAGATCCAC GCCTGAAATT TCCAGTACAC ATGCGGTCAA AGGCATCCTT AAACCCCAAT
    GACCTTGGCC CTCTTCCTCC TGGCTGGGAA GAAAGGATCC ACTTGGATGG CCGGACGTTT
    TACATTGACC ACAGCAGCCA GGTGTTATGT GGCGAGGACG ATGCCAGAGA CTCAGTGCCC
    TCGTACGATA GCAAAATTAC TCAGTGGGAA GACCCAAGAC TGCAGAACCC AGCTATTACT
    GGTCCGGCTG TTCCTTACTC CAGAGAATTT AAGCAGAAAT ATGACTACTT TAGAAAGAAA
    TTAAAGAAAC CTGCGGATAT TCCAAATAGG TTTGAAATGA AACTTCACAG AAATAACATA
    TTTGAAGAGT CCTATCGGAG AATCATGTCT GTGAAAAGAC CAGATGTCCT GAAAGCTAGG
    CTGTGGATTG AGTTTGAATC GGAGAAAGGT CTGGACTACG GGGGCGTGGC CAGAGAGTGG
    TTCTTCCTAC TGTCCAAGGA GATGTTCAAC CCTTACTACG GCCTCTTCGA GTATTCGGCA
    ACGGACAACT ACACACTTCA GATCAATCCC AATTCAGGCC TCTGTAATGA AGATCATTTG
    TCCTATTTCA CTTTCATTGG AAGAGTTGCT GGGCTGGCAG TATTTCATGG GAAACTTCTA
    GATGGTTTCT TCATTCGACC GTTTTATAAA ATGATGCTGG GAAAGCAGAT AACTCTGAAT
    GACATGGAAT CTGTGGATAG CGAATATTAC AACTCTTTGA AATGGATCTT AGAAAATGAC
    CCAACTGAAC TGGACCTTAT GTTCTGCATT GATGAAGAGA ACTTTGGGCA GACATATCAA
    GTGGATCTGA AGCCCAACGG GTCAGAAATA ATGGTAACGA ACGAAAACAA GAGAGAGTAC
    ATCGACCTCG TCATCCAGTG GAGGTTCGTG AACAGGGTCC AGAAGCAGAT GAACGCCTTC
    CTGGAGGGAT TCACGGAACT GCTCCCTATT GATTTGATTA AAATCTTTGA TGAAAATGAA
    CTGGAGTTGC TGATGTGTGG CCTTGGCGAC GTGGATGTGA ACGACTGGCG ACAACATTCT
    ATTTACAAGA ATGGCTACTG CCCGAACCAC CCCGTGATCC AGTGGTTCTG GAAGGCTGTG
    CTGCTGATGG ACGCCGAGAA GCGCATCCGG TTGCTGCAGT TTGTCACAGG GACGTCCCGA
    GTACCTATGA ATGGATTTGC CGAGCTTTAT GGTTCCAATG GTCCTCAGCT GTTTACAATA
    GAGCAATGGG GAAGTCCTGA AAAACTGCCC AGAGCTCACA CGTGCTTTAA TCGCCTTGAC
    TTACCTCCGT ATGAAACCTT CGAAGATTTA CGAGAGAAGC TCCTCATGGC TGTGGAAAAC
    GCCCAAGGCT TCGAGGGGGT GGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88370
    Clone ID Related Accession (Same CDS sequence) XM_005224355.3 , XM_005199757.3 , XM_005224355.4
    Accession Version XM_005224355.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2109bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104569.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005224355.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGGAAGATCC ACGCCTGAAA
    TTTCCAGTAC ACATGCGGTC AAAGGCATCC TTAAACCCCA ATGACCTTGG CCCTCTTCCT
    CCTGGCTGGG AAGAAAGGAT CCACTTGGAT GGCCGGACGT TTTACATTGA CCACAGCAGC
    CAGGTGTTAT GTGGCGAGGA CGATGCCAGA GACTCAGTGC CCTCGTACGA TAGCAAAATT
    ACTCAGTGGG AAGACCCAAG ACTGCAGAAC CCAGCTATTA CTGGTCCGGC TGTTCCTTAC
    TCCAGAGAAT TTAAGCAGAA ATATGACTAC TTTAGAAAGA AATTAAAGAA ACCTGCGGAT
    ATTCCAAATA GGTTTGAAAT GAAACTTCAC AGAAATAACA TATTTGAAGA GTCCTATCGG
    AGAATCATGT CTGTGAAAAG ACCAGATGTC CTGAAAGCTA GGCTGTGGAT TGAGTTTGAA
    TCGGAGAAAG GTCTGGACTA CGGGGGCGTG GCCAGAGAGT GGTTCTTCCT ACTGTCCAAG
    GAGATGTTCA ACCCTTACTA CGGCCTCTTC GAGTATTCGG CAACGGACAA CTACACACTT
    CAGATCAATC CCAATTCAGG CCTCTGTAAT GAAGATCATT TGTCCTATTT CACTTTCATT
    GGAAGAGTTG CTGGGCTGGC AGTATTTCAT GGGAAACTTC TAGATGGTTT CTTCATTCGA
    CCGTTTTATA AAATGATGCT GGGAAAGCAG ATAACTCTGA ATGACATGGA ATCTGTGGAT
    AGCGAATATT ACAACTCTTT GAAATGGATC TTAGAAAATG ACCCAACTGA ACTGGACCTT
    ATGTTCTGCA TTGATGAAGA GAACTTTGGG CAGACATATC AAGTGGATCT GAAGCCCAAC
    GGGTCAGAAA TAATGGTAAC GAACGAAAAC AAGAGAGAGT ACATCGACCT CGTCATCCAG
    TGGAGGTTCG TGAACAGGGT CCAGAAGCAG ATGAACGCCT TCCTGGAGGG ATTCACGGAA
    CTGCTCCCTA TTGATTTGAT TAAAATCTTT GATGAAAATG AACTGGAGTT GCTGATGTGT
    GGCCTTGGCG ACGTGGATGT GAACGACTGG CGACAACATT CTATTTACAA GAATGGCTAC
    TGCCCGAACC ACCCCGTGAT CCAGTGGTTC TGGAAGGCTG TGCTGCTGAT GGACGCCGAG
    AAGCGCATCC GGTTGCTGCA GTTTGTCACA GGGACGTCCC GAGTACCTAT GAATGGATTT
    GCCGAGCTTT ATGGTTCCAA TGGTCCTCAG CTGTTTACAA TAGAGCAATG GGGAAGTCCT
    GAAAAACTGC CCAGAGCTCA CACGTGCTTT AATCGCCTTG ACTTACCTCC GTATGAAACC
    TTCGAAGATT TACGAGAGAA GCTCCTCATG GCTGTGGAAA ACGCCCAAGG CTTCGAGGGG
    GTGGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005224412.3
    CDS565..2673
    Translation

    Target ORF information:

    RefSeq Version XM_005224355.3
    Organism Bos taurus(cattle)
    Definition Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005224355.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGGAAGATCC ACGCCTGAAA
    TTTCCAGTAC ACATGCGGTC AAAGGCATCC TTAAACCCCA ATGACCTTGG CCCTCTTCCT
    CCTGGCTGGG AAGAAAGGAT CCACTTGGAT GGCCGGACGT TTTACATTGA CCACAGCAGC
    CAGGTGTTAT GTGGCGAGGA CGATGCCAGA GACTCAGTGC CCTCGTACGA TAGCAAAATT
    ACTCAGTGGG AAGACCCAAG ACTGCAGAAC CCAGCTATTA CTGGTCCGGC TGTTCCTTAC
    TCCAGAGAAT TTAAGCAGAA ATATGACTAC TTTAGAAAGA AATTAAAGAA ACCTGCGGAT
    ATTCCAAATA GGTTTGAAAT GAAACTTCAC AGAAATAACA TATTTGAAGA GTCCTATCGG
    AGAATCATGT CTGTGAAAAG ACCAGATGTC CTGAAAGCTA GGCTGTGGAT TGAGTTTGAA
    TCGGAGAAAG GTCTGGACTA CGGGGGCGTG GCCAGAGAGT GGTTCTTCCT ACTGTCCAAG
    GAGATGTTCA ACCCTTACTA CGGCCTCTTC GAGTATTCGG CAACGGACAA CTACACACTT
    CAGATCAATC CCAATTCAGG CCTCTGTAAT GAAGATCATT TGTCCTATTT CACTTTCATT
    GGAAGAGTTG CTGGGCTGGC AGTATTTCAT GGGAAACTTC TAGATGGTTT CTTCATTCGA
    CCGTTTTATA AAATGATGCT GGGAAAGCAG ATAACTCTGA ATGACATGGA ATCTGTGGAT
    AGCGAATATT ACAACTCTTT GAAATGGATC TTAGAAAATG ACCCAACTGA ACTGGACCTT
    ATGTTCTGCA TTGATGAAGA GAACTTTGGG CAGACATATC AAGTGGATCT GAAGCCCAAC
    GGGTCAGAAA TAATGGTAAC GAACGAAAAC AAGAGAGAGT ACATCGACCT CGTCATCCAG
    TGGAGGTTCG TGAACAGGGT CCAGAAGCAG ATGAACGCCT TCCTGGAGGG ATTCACGGAA
    CTGCTCCCTA TTGATTTGAT TAAAATCTTT GATGAAAATG AACTGGAGTT GCTGATGTGT
    GGCCTTGGCG ACGTGGATGT GAACGACTGG CGACAACATT CTATTTACAA GAATGGCTAC
    TGCCCGAACC ACCCCGTGAT CCAGTGGTTC TGGAAGGCTG TGCTGCTGAT GGACGCCGAG
    AAGCGCATCC GGTTGCTGCA GTTTGTCACA GGGACGTCCC GAGTACCTAT GAATGGATTT
    GCCGAGCTTT ATGGTTCCAA TGGTCCTCAG CTGTTTACAA TAGAGCAATG GGGAAGTCCT
    GAAAAACTGC CCAGAGCTCA CACGTGCTTT AATCGCCTTG ACTTACCTCC GTATGAAACC
    TTCGAAGATT TACGAGAGAA GCTCCTCATG GCTGTGGAAA ACGCCCAAGG CTTCGAGGGG
    GTGGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88370
    Clone ID Related Accession (Same CDS sequence) XM_005224355.3 , XM_005199757.3 , XM_005224355.4
    Accession Version XM_005199757.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2109bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646636.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005199757.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGGAAGATCC ACGCCTGAAA
    TTTCCAGTAC ACATGCGGTC AAAGGCATCC TTAAACCCCA ATGACCTTGG CCCTCTTCCT
    CCTGGCTGGG AAGAAAGGAT CCACTTGGAT GGCCGGACGT TTTACATTGA CCACAGCAGC
    CAGGTGTTAT GTGGCGAGGA CGATGCCAGA GACTCAGTGC CCTCGTACGA TAGCAAAATT
    ACTCAGTGGG AAGACCCAAG ACTGCAGAAC CCAGCTATTA CTGGTCCGGC TGTTCCTTAC
    TCCAGAGAAT TTAAGCAGAA ATATGACTAC TTTAGAAAGA AATTAAAGAA ACCTGCGGAT
    ATTCCAAATA GGTTTGAAAT GAAACTTCAC AGAAATAACA TATTTGAAGA GTCCTATCGG
    AGAATCATGT CTGTGAAAAG ACCAGATGTC CTGAAAGCTA GGCTGTGGAT TGAGTTTGAA
    TCGGAGAAAG GTCTGGACTA CGGGGGCGTG GCCAGAGAGT GGTTCTTCCT ACTGTCCAAG
    GAGATGTTCA ACCCTTACTA CGGCCTCTTC GAGTATTCGG CAACGGACAA CTACACACTT
    CAGATCAATC CCAATTCAGG CCTCTGTAAT GAAGATCATT TGTCCTATTT CACTTTCATT
    GGAAGAGTTG CTGGGCTGGC AGTATTTCAT GGGAAACTTC TAGATGGTTT CTTCATTCGA
    CCGTTTTATA AAATGATGCT GGGAAAGCAG ATAACTCTGA ATGACATGGA ATCTGTGGAT
    AGCGAATATT ACAACTCTTT GAAATGGATC TTAGAAAATG ACCCAACTGA ACTGGACCTT
    ATGTTCTGCA TTGATGAAGA GAACTTTGGG CAGACATATC AAGTGGATCT GAAGCCCAAC
    GGGTCAGAAA TAATGGTAAC GAACGAAAAC AAGAGAGAGT ACATCGACCT CGTCATCCAG
    TGGAGGTTCG TGAACAGGGT CCAGAAGCAG ATGAACGCCT TCCTGGAGGG ATTCACGGAA
    CTGCTCCCTA TTGATTTGAT TAAAATCTTT GATGAAAATG AACTGGAGTT GCTGATGTGT
    GGCCTTGGCG ACGTGGATGT GAACGACTGG CGACAACATT CTATTTACAA GAATGGCTAC
    TGCCCGAACC ACCCCGTGAT CCAGTGGTTC TGGAAGGCTG TGCTGCTGAT GGACGCCGAG
    AAGCGCATCC GGTTGCTGCA GTTTGTCACA GGGACGTCCC GAGTACCTAT GAATGGATTT
    GCCGAGCTTT ATGGTTCCAA TGGTCCTCAG CTGTTTACAA TAGAGCAATG GGGAAGTCCT
    GAAAAACTGC CCAGAGCTCA CACGTGCTTT AATCGCCTTG ACTTACCTCC GTATGAAACC
    TTCGAAGATT TACGAGAGAA GCTCCTCATG GCTGTGGAAA ACGCCCAAGG CTTCGAGGGG
    GTGGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005199814.3
    CDS565..2673
    Translation

    Target ORF information:

    RefSeq Version XM_005199757.3
    Organism Bos taurus(cattle)
    Definition Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005199757.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGGAAGATCC ACGCCTGAAA
    TTTCCAGTAC ACATGCGGTC AAAGGCATCC TTAAACCCCA ATGACCTTGG CCCTCTTCCT
    CCTGGCTGGG AAGAAAGGAT CCACTTGGAT GGCCGGACGT TTTACATTGA CCACAGCAGC
    CAGGTGTTAT GTGGCGAGGA CGATGCCAGA GACTCAGTGC CCTCGTACGA TAGCAAAATT
    ACTCAGTGGG AAGACCCAAG ACTGCAGAAC CCAGCTATTA CTGGTCCGGC TGTTCCTTAC
    TCCAGAGAAT TTAAGCAGAA ATATGACTAC TTTAGAAAGA AATTAAAGAA ACCTGCGGAT
    ATTCCAAATA GGTTTGAAAT GAAACTTCAC AGAAATAACA TATTTGAAGA GTCCTATCGG
    AGAATCATGT CTGTGAAAAG ACCAGATGTC CTGAAAGCTA GGCTGTGGAT TGAGTTTGAA
    TCGGAGAAAG GTCTGGACTA CGGGGGCGTG GCCAGAGAGT GGTTCTTCCT ACTGTCCAAG
    GAGATGTTCA ACCCTTACTA CGGCCTCTTC GAGTATTCGG CAACGGACAA CTACACACTT
    CAGATCAATC CCAATTCAGG CCTCTGTAAT GAAGATCATT TGTCCTATTT CACTTTCATT
    GGAAGAGTTG CTGGGCTGGC AGTATTTCAT GGGAAACTTC TAGATGGTTT CTTCATTCGA
    CCGTTTTATA AAATGATGCT GGGAAAGCAG ATAACTCTGA ATGACATGGA ATCTGTGGAT
    AGCGAATATT ACAACTCTTT GAAATGGATC TTAGAAAATG ACCCAACTGA ACTGGACCTT
    ATGTTCTGCA TTGATGAAGA GAACTTTGGG CAGACATATC AAGTGGATCT GAAGCCCAAC
    GGGTCAGAAA TAATGGTAAC GAACGAAAAC AAGAGAGAGT ACATCGACCT CGTCATCCAG
    TGGAGGTTCG TGAACAGGGT CCAGAAGCAG ATGAACGCCT TCCTGGAGGG ATTCACGGAA
    CTGCTCCCTA TTGATTTGAT TAAAATCTTT GATGAAAATG AACTGGAGTT GCTGATGTGT
    GGCCTTGGCG ACGTGGATGT GAACGACTGG CGACAACATT CTATTTACAA GAATGGCTAC
    TGCCCGAACC ACCCCGTGAT CCAGTGGTTC TGGAAGGCTG TGCTGCTGAT GGACGCCGAG
    AAGCGCATCC GGTTGCTGCA GTTTGTCACA GGGACGTCCC GAGTACCTAT GAATGGATTT
    GCCGAGCTTT ATGGTTCCAA TGGTCCTCAG CTGTTTACAA TAGAGCAATG GGGAAGTCCT
    GAAAAACTGC CCAGAGCTCA CACGTGCTTT AATCGCCTTG ACTTACCTCC GTATGAAACC
    TTCGAAGATT TACGAGAGAA GCTCCTCATG GCTGTGGAAA ACGCCCAAGG CTTCGAGGGG
    GTGGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88370
    Clone ID Related Accession (Same CDS sequence) XM_005224355.3 , XM_005199757.3 , XM_005224355.4
    Accession Version XM_005224355.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2109bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005224355.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGGAAGATCC ACGCCTGAAA
    TTTCCAGTAC ACATGCGGTC AAAGGCATCC TTAAACCCCA ATGACCTTGG CCCTCTTCCT
    CCTGGCTGGG AAGAAAGGAT CCACTTGGAT GGCCGGACGT TTTACATTGA CCACAGCAGC
    CAGGTGTTAT GTGGCGAGGA CGATGCCAGA GACTCAGTGC CCTCGTACGA TAGCAAAATT
    ACTCAGTGGG AAGACCCAAG ACTGCAGAAC CCAGCTATTA CTGGTCCGGC TGTTCCTTAC
    TCCAGAGAAT TTAAGCAGAA ATATGACTAC TTTAGAAAGA AATTAAAGAA ACCTGCGGAT
    ATTCCAAATA GGTTTGAAAT GAAACTTCAC AGAAATAACA TATTTGAAGA GTCCTATCGG
    AGAATCATGT CTGTGAAAAG ACCAGATGTC CTGAAAGCTA GGCTGTGGAT TGAGTTTGAA
    TCGGAGAAAG GTCTGGACTA CGGGGGCGTG GCCAGAGAGT GGTTCTTCCT ACTGTCCAAG
    GAGATGTTCA ACCCTTACTA CGGCCTCTTC GAGTATTCGG CAACGGACAA CTACACACTT
    CAGATCAATC CCAATTCAGG CCTCTGTAAT GAAGATCATT TGTCCTATTT CACTTTCATT
    GGAAGAGTTG CTGGGCTGGC AGTATTTCAT GGGAAACTTC TAGATGGTTT CTTCATTCGA
    CCGTTTTATA AAATGATGCT GGGAAAGCAG ATAACTCTGA ATGACATGGA ATCTGTGGAT
    AGCGAATATT ACAACTCTTT GAAATGGATC TTAGAAAATG ACCCAACTGA ACTGGACCTT
    ATGTTCTGCA TTGATGAAGA GAACTTTGGG CAGACATATC AAGTGGATCT GAAGCCCAAC
    GGGTCAGAAA TAATGGTAAC GAACGAAAAC AAGAGAGAGT ACATCGACCT CGTCATCCAG
    TGGAGGTTCG TGAACAGGGT CCAGAAGCAG ATGAACGCCT TCCTGGAGGG ATTCACGGAA
    CTGCTCCCTA TTGATTTGAT TAAAATCTTT GATGAAAATG AACTGGAGTT GCTGATGTGT
    GGCCTTGGCG ACGTGGATGT GAACGACTGG CGACAACATT CTATTTACAA GAATGGCTAC
    TGCCCGAACC ACCCCGTGAT CCAGTGGTTC TGGAAGGCTG TGCTGCTGAT GGACGCCGAG
    AAGCGCATCC GGTTGCTGCA GTTTGTCACA GGGACGTCCC GAGTACCTAT GAATGGATTT
    GCCGAGCTTT ATGGTTCCAA TGGTCCTCAG CTGTTTACAA TAGAGCAATG GGGAAGTCCT
    GAAAAACTGC CCAGAGCTCA CACGTGCTTT AATCGCCTTG ACTTACCTCC GTATGAAACC
    TTCGAAGATT TACGAGAGAA GCTCCTCATG GCTGTGGAAA ACGCCCAAGG CTTCGAGGGG
    GTGGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005224412.3
    CDS568..2676
    Translation

    Target ORF information:

    RefSeq Version XM_005224355.4
    Organism Bos taurus(cattle)
    Definition Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005224355.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGGAAGATCC ACGCCTGAAA
    TTTCCAGTAC ACATGCGGTC AAAGGCATCC TTAAACCCCA ATGACCTTGG CCCTCTTCCT
    CCTGGCTGGG AAGAAAGGAT CCACTTGGAT GGCCGGACGT TTTACATTGA CCACAGCAGC
    CAGGTGTTAT GTGGCGAGGA CGATGCCAGA GACTCAGTGC CCTCGTACGA TAGCAAAATT
    ACTCAGTGGG AAGACCCAAG ACTGCAGAAC CCAGCTATTA CTGGTCCGGC TGTTCCTTAC
    TCCAGAGAAT TTAAGCAGAA ATATGACTAC TTTAGAAAGA AATTAAAGAA ACCTGCGGAT
    ATTCCAAATA GGTTTGAAAT GAAACTTCAC AGAAATAACA TATTTGAAGA GTCCTATCGG
    AGAATCATGT CTGTGAAAAG ACCAGATGTC CTGAAAGCTA GGCTGTGGAT TGAGTTTGAA
    TCGGAGAAAG GTCTGGACTA CGGGGGCGTG GCCAGAGAGT GGTTCTTCCT ACTGTCCAAG
    GAGATGTTCA ACCCTTACTA CGGCCTCTTC GAGTATTCGG CAACGGACAA CTACACACTT
    CAGATCAATC CCAATTCAGG CCTCTGTAAT GAAGATCATT TGTCCTATTT CACTTTCATT
    GGAAGAGTTG CTGGGCTGGC AGTATTTCAT GGGAAACTTC TAGATGGTTT CTTCATTCGA
    CCGTTTTATA AAATGATGCT GGGAAAGCAG ATAACTCTGA ATGACATGGA ATCTGTGGAT
    AGCGAATATT ACAACTCTTT GAAATGGATC TTAGAAAATG ACCCAACTGA ACTGGACCTT
    ATGTTCTGCA TTGATGAAGA GAACTTTGGG CAGACATATC AAGTGGATCT GAAGCCCAAC
    GGGTCAGAAA TAATGGTAAC GAACGAAAAC AAGAGAGAGT ACATCGACCT CGTCATCCAG
    TGGAGGTTCG TGAACAGGGT CCAGAAGCAG ATGAACGCCT TCCTGGAGGG ATTCACGGAA
    CTGCTCCCTA TTGATTTGAT TAAAATCTTT GATGAAAATG AACTGGAGTT GCTGATGTGT
    GGCCTTGGCG ACGTGGATGT GAACGACTGG CGACAACATT CTATTTACAA GAATGGCTAC
    TGCCCGAACC ACCCCGTGAT CCAGTGGTTC TGGAAGGCTG TGCTGCTGAT GGACGCCGAG
    AAGCGCATCC GGTTGCTGCA GTTTGTCACA GGGACGTCCC GAGTACCTAT GAATGGATTT
    GCCGAGCTTT ATGGTTCCAA TGGTCCTCAG CTGTTTACAA TAGAGCAATG GGGAAGTCCT
    GAAAAACTGC CCAGAGCTCA CACGTGCTTT AATCGCCTTG ACTTACCTCC GTATGAAACC
    TTCGAAGATT TACGAGAGAA GCTCCTCATG GCTGTGGAAA ACGCCCAAGG CTTCGAGGGG
    GTGGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88371
    Clone ID Related Accession (Same CDS sequence) XM_005224356.3 , XM_005199758.3 , XM_005224356.4
    Accession Version XM_005224356.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2055bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104569.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005224356.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGGAAGATCC ACGCCTGAAA
    TTTCCAGTAC ACATGCGGTC AAAGGCATCC TTAAACCCCA ATGACCTTGG CCCTCTTCCT
    CCTGGCTGGG AAGAAAGGAT CCACTTGGAT GGCCGGACGT TTTACATTGA CCACAATAGC
    AAAATTACTC AGTGGGAAGA CCCAAGACTG CAGAACCCAG CTATTACTGG TCCGGCTGTT
    CCTTACTCCA GAGAATTTAA GCAGAAATAT GACTACTTTA GAAAGAAATT AAAGAAACCT
    GCGGATATTC CAAATAGGTT TGAAATGAAA CTTCACAGAA ATAACATATT TGAAGAGTCC
    TATCGGAGAA TCATGTCTGT GAAAAGACCA GATGTCCTGA AAGCTAGGCT GTGGATTGAG
    TTTGAATCGG AGAAAGGTCT GGACTACGGG GGCGTGGCCA GAGAGTGGTT CTTCCTACTG
    TCCAAGGAGA TGTTCAACCC TTACTACGGC CTCTTCGAGT ATTCGGCAAC GGACAACTAC
    ACACTTCAGA TCAATCCCAA TTCAGGCCTC TGTAATGAAG ATCATTTGTC CTATTTCACT
    TTCATTGGAA GAGTTGCTGG GCTGGCAGTA TTTCATGGGA AACTTCTAGA TGGTTTCTTC
    ATTCGACCGT TTTATAAAAT GATGCTGGGA AAGCAGATAA CTCTGAATGA CATGGAATCT
    GTGGATAGCG AATATTACAA CTCTTTGAAA TGGATCTTAG AAAATGACCC AACTGAACTG
    GACCTTATGT TCTGCATTGA TGAAGAGAAC TTTGGGCAGA CATATCAAGT GGATCTGAAG
    CCCAACGGGT CAGAAATAAT GGTAACGAAC GAAAACAAGA GAGAGTACAT CGACCTCGTC
    ATCCAGTGGA GGTTCGTGAA CAGGGTCCAG AAGCAGATGA ACGCCTTCCT GGAGGGATTC
    ACGGAACTGC TCCCTATTGA TTTGATTAAA ATCTTTGATG AAAATGAACT GGAGTTGCTG
    ATGTGTGGCC TTGGCGACGT GGATGTGAAC GACTGGCGAC AACATTCTAT TTACAAGAAT
    GGCTACTGCC CGAACCACCC CGTGATCCAG TGGTTCTGGA AGGCTGTGCT GCTGATGGAC
    GCCGAGAAGC GCATCCGGTT GCTGCAGTTT GTCACAGGGA CGTCCCGAGT ACCTATGAAT
    GGATTTGCCG AGCTTTATGG TTCCAATGGT CCTCAGCTGT TTACAATAGA GCAATGGGGA
    AGTCCTGAAA AACTGCCCAG AGCTCACACG TGCTTTAATC GCCTTGACTT ACCTCCGTAT
    GAAACCTTCG AAGATTTACG AGAGAAGCTC CTCATGGCTG TGGAAAACGC CCAAGGCTTC
    GAGGGGGTGG ATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005224413.3
    CDS559..2613
    Translation

    Target ORF information:

    RefSeq Version XM_005224356.3
    Organism Bos taurus(cattle)
    Definition Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005224356.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGGAAGATCC ACGCCTGAAA
    TTTCCAGTAC ACATGCGGTC AAAGGCATCC TTAAACCCCA ATGACCTTGG CCCTCTTCCT
    CCTGGCTGGG AAGAAAGGAT CCACTTGGAT GGCCGGACGT TTTACATTGA CCACAATAGC
    AAAATTACTC AGTGGGAAGA CCCAAGACTG CAGAACCCAG CTATTACTGG TCCGGCTGTT
    CCTTACTCCA GAGAATTTAA GCAGAAATAT GACTACTTTA GAAAGAAATT AAAGAAACCT
    GCGGATATTC CAAATAGGTT TGAAATGAAA CTTCACAGAA ATAACATATT TGAAGAGTCC
    TATCGGAGAA TCATGTCTGT GAAAAGACCA GATGTCCTGA AAGCTAGGCT GTGGATTGAG
    TTTGAATCGG AGAAAGGTCT GGACTACGGG GGCGTGGCCA GAGAGTGGTT CTTCCTACTG
    TCCAAGGAGA TGTTCAACCC TTACTACGGC CTCTTCGAGT ATTCGGCAAC GGACAACTAC
    ACACTTCAGA TCAATCCCAA TTCAGGCCTC TGTAATGAAG ATCATTTGTC CTATTTCACT
    TTCATTGGAA GAGTTGCTGG GCTGGCAGTA TTTCATGGGA AACTTCTAGA TGGTTTCTTC
    ATTCGACCGT TTTATAAAAT GATGCTGGGA AAGCAGATAA CTCTGAATGA CATGGAATCT
    GTGGATAGCG AATATTACAA CTCTTTGAAA TGGATCTTAG AAAATGACCC AACTGAACTG
    GACCTTATGT TCTGCATTGA TGAAGAGAAC TTTGGGCAGA CATATCAAGT GGATCTGAAG
    CCCAACGGGT CAGAAATAAT GGTAACGAAC GAAAACAAGA GAGAGTACAT CGACCTCGTC
    ATCCAGTGGA GGTTCGTGAA CAGGGTCCAG AAGCAGATGA ACGCCTTCCT GGAGGGATTC
    ACGGAACTGC TCCCTATTGA TTTGATTAAA ATCTTTGATG AAAATGAACT GGAGTTGCTG
    ATGTGTGGCC TTGGCGACGT GGATGTGAAC GACTGGCGAC AACATTCTAT TTACAAGAAT
    GGCTACTGCC CGAACCACCC CGTGATCCAG TGGTTCTGGA AGGCTGTGCT GCTGATGGAC
    GCCGAGAAGC GCATCCGGTT GCTGCAGTTT GTCACAGGGA CGTCCCGAGT ACCTATGAAT
    GGATTTGCCG AGCTTTATGG TTCCAATGGT CCTCAGCTGT TTACAATAGA GCAATGGGGA
    AGTCCTGAAA AACTGCCCAG AGCTCACACG TGCTTTAATC GCCTTGACTT ACCTCCGTAT
    GAAACCTTCG AAGATTTACG AGAGAAGCTC CTCATGGCTG TGGAAAACGC CCAAGGCTTC
    GAGGGGGTGG ATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88371
    Clone ID Related Accession (Same CDS sequence) XM_005224356.3 , XM_005199758.3 , XM_005224356.4
    Accession Version XM_005199758.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2055bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646636.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005199758.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGGAAGATCC ACGCCTGAAA
    TTTCCAGTAC ACATGCGGTC AAAGGCATCC TTAAACCCCA ATGACCTTGG CCCTCTTCCT
    CCTGGCTGGG AAGAAAGGAT CCACTTGGAT GGCCGGACGT TTTACATTGA CCACAATAGC
    AAAATTACTC AGTGGGAAGA CCCAAGACTG CAGAACCCAG CTATTACTGG TCCGGCTGTT
    CCTTACTCCA GAGAATTTAA GCAGAAATAT GACTACTTTA GAAAGAAATT AAAGAAACCT
    GCGGATATTC CAAATAGGTT TGAAATGAAA CTTCACAGAA ATAACATATT TGAAGAGTCC
    TATCGGAGAA TCATGTCTGT GAAAAGACCA GATGTCCTGA AAGCTAGGCT GTGGATTGAG
    TTTGAATCGG AGAAAGGTCT GGACTACGGG GGCGTGGCCA GAGAGTGGTT CTTCCTACTG
    TCCAAGGAGA TGTTCAACCC TTACTACGGC CTCTTCGAGT ATTCGGCAAC GGACAACTAC
    ACACTTCAGA TCAATCCCAA TTCAGGCCTC TGTAATGAAG ATCATTTGTC CTATTTCACT
    TTCATTGGAA GAGTTGCTGG GCTGGCAGTA TTTCATGGGA AACTTCTAGA TGGTTTCTTC
    ATTCGACCGT TTTATAAAAT GATGCTGGGA AAGCAGATAA CTCTGAATGA CATGGAATCT
    GTGGATAGCG AATATTACAA CTCTTTGAAA TGGATCTTAG AAAATGACCC AACTGAACTG
    GACCTTATGT TCTGCATTGA TGAAGAGAAC TTTGGGCAGA CATATCAAGT GGATCTGAAG
    CCCAACGGGT CAGAAATAAT GGTAACGAAC GAAAACAAGA GAGAGTACAT CGACCTCGTC
    ATCCAGTGGA GGTTCGTGAA CAGGGTCCAG AAGCAGATGA ACGCCTTCCT GGAGGGATTC
    ACGGAACTGC TCCCTATTGA TTTGATTAAA ATCTTTGATG AAAATGAACT GGAGTTGCTG
    ATGTGTGGCC TTGGCGACGT GGATGTGAAC GACTGGCGAC AACATTCTAT TTACAAGAAT
    GGCTACTGCC CGAACCACCC CGTGATCCAG TGGTTCTGGA AGGCTGTGCT GCTGATGGAC
    GCCGAGAAGC GCATCCGGTT GCTGCAGTTT GTCACAGGGA CGTCCCGAGT ACCTATGAAT
    GGATTTGCCG AGCTTTATGG TTCCAATGGT CCTCAGCTGT TTACAATAGA GCAATGGGGA
    AGTCCTGAAA AACTGCCCAG AGCTCACACG TGCTTTAATC GCCTTGACTT ACCTCCGTAT
    GAAACCTTCG AAGATTTACG AGAGAAGCTC CTCATGGCTG TGGAAAACGC CCAAGGCTTC
    GAGGGGGTGG ATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005199815.3
    CDS559..2613
    Translation

    Target ORF information:

    RefSeq Version XM_005199758.3
    Organism Bos taurus(cattle)
    Definition Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005199758.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGGAAGATCC ACGCCTGAAA
    TTTCCAGTAC ACATGCGGTC AAAGGCATCC TTAAACCCCA ATGACCTTGG CCCTCTTCCT
    CCTGGCTGGG AAGAAAGGAT CCACTTGGAT GGCCGGACGT TTTACATTGA CCACAATAGC
    AAAATTACTC AGTGGGAAGA CCCAAGACTG CAGAACCCAG CTATTACTGG TCCGGCTGTT
    CCTTACTCCA GAGAATTTAA GCAGAAATAT GACTACTTTA GAAAGAAATT AAAGAAACCT
    GCGGATATTC CAAATAGGTT TGAAATGAAA CTTCACAGAA ATAACATATT TGAAGAGTCC
    TATCGGAGAA TCATGTCTGT GAAAAGACCA GATGTCCTGA AAGCTAGGCT GTGGATTGAG
    TTTGAATCGG AGAAAGGTCT GGACTACGGG GGCGTGGCCA GAGAGTGGTT CTTCCTACTG
    TCCAAGGAGA TGTTCAACCC TTACTACGGC CTCTTCGAGT ATTCGGCAAC GGACAACTAC
    ACACTTCAGA TCAATCCCAA TTCAGGCCTC TGTAATGAAG ATCATTTGTC CTATTTCACT
    TTCATTGGAA GAGTTGCTGG GCTGGCAGTA TTTCATGGGA AACTTCTAGA TGGTTTCTTC
    ATTCGACCGT TTTATAAAAT GATGCTGGGA AAGCAGATAA CTCTGAATGA CATGGAATCT
    GTGGATAGCG AATATTACAA CTCTTTGAAA TGGATCTTAG AAAATGACCC AACTGAACTG
    GACCTTATGT TCTGCATTGA TGAAGAGAAC TTTGGGCAGA CATATCAAGT GGATCTGAAG
    CCCAACGGGT CAGAAATAAT GGTAACGAAC GAAAACAAGA GAGAGTACAT CGACCTCGTC
    ATCCAGTGGA GGTTCGTGAA CAGGGTCCAG AAGCAGATGA ACGCCTTCCT GGAGGGATTC
    ACGGAACTGC TCCCTATTGA TTTGATTAAA ATCTTTGATG AAAATGAACT GGAGTTGCTG
    ATGTGTGGCC TTGGCGACGT GGATGTGAAC GACTGGCGAC AACATTCTAT TTACAAGAAT
    GGCTACTGCC CGAACCACCC CGTGATCCAG TGGTTCTGGA AGGCTGTGCT GCTGATGGAC
    GCCGAGAAGC GCATCCGGTT GCTGCAGTTT GTCACAGGGA CGTCCCGAGT ACCTATGAAT
    GGATTTGCCG AGCTTTATGG TTCCAATGGT CCTCAGCTGT TTACAATAGA GCAATGGGGA
    AGTCCTGAAA AACTGCCCAG AGCTCACACG TGCTTTAATC GCCTTGACTT ACCTCCGTAT
    GAAACCTTCG AAGATTTACG AGAGAAGCTC CTCATGGCTG TGGAAAACGC CCAAGGCTTC
    GAGGGGGTGG ATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88371
    Clone ID Related Accession (Same CDS sequence) XM_005224356.3 , XM_005199758.3 , XM_005224356.4
    Accession Version XM_005224356.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2055bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005224356.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGGAAGATCC ACGCCTGAAA
    TTTCCAGTAC ACATGCGGTC AAAGGCATCC TTAAACCCCA ATGACCTTGG CCCTCTTCCT
    CCTGGCTGGG AAGAAAGGAT CCACTTGGAT GGCCGGACGT TTTACATTGA CCACAATAGC
    AAAATTACTC AGTGGGAAGA CCCAAGACTG CAGAACCCAG CTATTACTGG TCCGGCTGTT
    CCTTACTCCA GAGAATTTAA GCAGAAATAT GACTACTTTA GAAAGAAATT AAAGAAACCT
    GCGGATATTC CAAATAGGTT TGAAATGAAA CTTCACAGAA ATAACATATT TGAAGAGTCC
    TATCGGAGAA TCATGTCTGT GAAAAGACCA GATGTCCTGA AAGCTAGGCT GTGGATTGAG
    TTTGAATCGG AGAAAGGTCT GGACTACGGG GGCGTGGCCA GAGAGTGGTT CTTCCTACTG
    TCCAAGGAGA TGTTCAACCC TTACTACGGC CTCTTCGAGT ATTCGGCAAC GGACAACTAC
    ACACTTCAGA TCAATCCCAA TTCAGGCCTC TGTAATGAAG ATCATTTGTC CTATTTCACT
    TTCATTGGAA GAGTTGCTGG GCTGGCAGTA TTTCATGGGA AACTTCTAGA TGGTTTCTTC
    ATTCGACCGT TTTATAAAAT GATGCTGGGA AAGCAGATAA CTCTGAATGA CATGGAATCT
    GTGGATAGCG AATATTACAA CTCTTTGAAA TGGATCTTAG AAAATGACCC AACTGAACTG
    GACCTTATGT TCTGCATTGA TGAAGAGAAC TTTGGGCAGA CATATCAAGT GGATCTGAAG
    CCCAACGGGT CAGAAATAAT GGTAACGAAC GAAAACAAGA GAGAGTACAT CGACCTCGTC
    ATCCAGTGGA GGTTCGTGAA CAGGGTCCAG AAGCAGATGA ACGCCTTCCT GGAGGGATTC
    ACGGAACTGC TCCCTATTGA TTTGATTAAA ATCTTTGATG AAAATGAACT GGAGTTGCTG
    ATGTGTGGCC TTGGCGACGT GGATGTGAAC GACTGGCGAC AACATTCTAT TTACAAGAAT
    GGCTACTGCC CGAACCACCC CGTGATCCAG TGGTTCTGGA AGGCTGTGCT GCTGATGGAC
    GCCGAGAAGC GCATCCGGTT GCTGCAGTTT GTCACAGGGA CGTCCCGAGT ACCTATGAAT
    GGATTTGCCG AGCTTTATGG TTCCAATGGT CCTCAGCTGT TTACAATAGA GCAATGGGGA
    AGTCCTGAAA AACTGCCCAG AGCTCACACG TGCTTTAATC GCCTTGACTT ACCTCCGTAT
    GAAACCTTCG AAGATTTACG AGAGAAGCTC CTCATGGCTG TGGAAAACGC CCAAGGCTTC
    GAGGGGGTGG ATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005224413.3
    CDS561..2615
    Translation

    Target ORF information:

    RefSeq Version XM_005224356.4
    Organism Bos taurus(cattle)
    Definition Bos taurus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005224356.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCCA AAAACGGAGG CCAAGATGAA
    GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC
    TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAG
    AAGGTGGACA ACCTGGGCCG CACGTACTAC GTCAACCACA ACAACCGGAC CACCCAGTGG
    CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC
    CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG
    GAGCCCAGCG AGGGCGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC
    ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CGGCCTCGCC CGTGTCCCGG
    ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC
    AACGGCGAGC AGTTTGGGTC TTTGATTCAG AGAGAGCCGT CCTCCAGGCT GAGGTCCTGC
    AGTGTCACCG ACGCAGTCGC GGAGCAGGCT CACCTCCCGC CGGAAGATCC ACGCCTGAAA
    TTTCCAGTAC ACATGCGGTC AAAGGCATCC TTAAACCCCA ATGACCTTGG CCCTCTTCCT
    CCTGGCTGGG AAGAAAGGAT CCACTTGGAT GGCCGGACGT TTTACATTGA CCACAATAGC
    AAAATTACTC AGTGGGAAGA CCCAAGACTG CAGAACCCAG CTATTACTGG TCCGGCTGTT
    CCTTACTCCA GAGAATTTAA GCAGAAATAT GACTACTTTA GAAAGAAATT AAAGAAACCT
    GCGGATATTC CAAATAGGTT TGAAATGAAA CTTCACAGAA ATAACATATT TGAAGAGTCC
    TATCGGAGAA TCATGTCTGT GAAAAGACCA GATGTCCTGA AAGCTAGGCT GTGGATTGAG
    TTTGAATCGG AGAAAGGTCT GGACTACGGG GGCGTGGCCA GAGAGTGGTT CTTCCTACTG
    TCCAAGGAGA TGTTCAACCC TTACTACGGC CTCTTCGAGT ATTCGGCAAC GGACAACTAC
    ACACTTCAGA TCAATCCCAA TTCAGGCCTC TGTAATGAAG ATCATTTGTC CTATTTCACT
    TTCATTGGAA GAGTTGCTGG GCTGGCAGTA TTTCATGGGA AACTTCTAGA TGGTTTCTTC
    ATTCGACCGT TTTATAAAAT GATGCTGGGA AAGCAGATAA CTCTGAATGA CATGGAATCT
    GTGGATAGCG AATATTACAA CTCTTTGAAA TGGATCTTAG AAAATGACCC AACTGAACTG
    GACCTTATGT TCTGCATTGA TGAAGAGAAC TTTGGGCAGA CATATCAAGT GGATCTGAAG
    CCCAACGGGT CAGAAATAAT GGTAACGAAC GAAAACAAGA GAGAGTACAT CGACCTCGTC
    ATCCAGTGGA GGTTCGTGAA CAGGGTCCAG AAGCAGATGA ACGCCTTCCT GGAGGGATTC
    ACGGAACTGC TCCCTATTGA TTTGATTAAA ATCTTTGATG AAAATGAACT GGAGTTGCTG
    ATGTGTGGCC TTGGCGACGT GGATGTGAAC GACTGGCGAC AACATTCTAT TTACAAGAAT
    GGCTACTGCC CGAACCACCC CGTGATCCAG TGGTTCTGGA AGGCTGTGCT GCTGATGGAC
    GCCGAGAAGC GCATCCGGTT GCTGCAGTTT GTCACAGGGA CGTCCCGAGT ACCTATGAAT
    GGATTTGCCG AGCTTTATGG TTCCAATGGT CCTCAGCTGT TTACAATAGA GCAATGGGGA
    AGTCCTGAAA AACTGCCCAG AGCTCACACG TGCTTTAATC GCCTTGACTT ACCTCCGTAT
    GAAACCTTCG AAGATTTACG AGAGAAGCTC CTCATGGCTG TGGAAAACGC CCAAGGCTTC
    GAGGGGGTGG ATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.