NEDD4L cDNA ORF clone, Homo sapiens(human)

The following NEDD4L gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NEDD4L cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu14306 NM_001144966.3
Latest version!
Homo sapiens NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant a, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$339.50-$475.30
$679.00
OHu14306 NM_001144964.1
Latest version!
Homo sapiens NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant b, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$339.50-$475.30
$679.00
OHu14306 NM_001144965.2
Latest version!
Homo sapiens NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant f, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$339.50-$475.30
$679.00
OHu21352 NM_001144967.3
Latest version!
Homo sapiens NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant j, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$339.50-$475.30
$679.00
OHu14306 XM_017025679.2
Latest version!
Homo sapiens NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant X20, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$339.50-$475.30
$679.00
OHu14306 XM_024451129.1
Latest version!
Homo sapiens NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$339.50-$475.30
$679.00
OHu14254 NM_001144971.2
Latest version!
Homo sapiens NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant k, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $587.30
$839.00
OHu14254 NM_001144970.3
Latest version!
Homo sapiens NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant c, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $587.30
$839.00
OHu14336 NM_001144968.2
Latest version!
Homo sapiens NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant g, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $657.30
$939.00
OHu14499 NM_001144969.2
Latest version!
Homo sapiens NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant h, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $657.30
$939.00
OHu14655 NM_001243960.2
Latest version!
Homo sapiens NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant i, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu14742 NM_015277.6
Latest version!
Homo sapiens NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant d, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $657.30
$939.00
OHu14254 XM_024451137.1
Latest version!
Homo sapiens NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant X23, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $587.30
$839.00
OHu14254 XM_024451136.1
Latest version!
Homo sapiens NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant X22, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $587.30
$839.00
OHu14254 XM_024451135.1
Latest version!
Homo sapiens NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant X21, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $587.30
$839.00
OHu14254 XM_024451131.1
Latest version!
Homo sapiens NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $587.30
$839.00
OHu51146 XM_006722428.4
Latest version!
Homo sapiens NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu51148 XM_006722430.4
Latest version!
Homo sapiens NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu51148 XM_024451134.1
Latest version!
Homo sapiens NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu51148 XM_024451133.1
Latest version!
Homo sapiens NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu65973 XM_005266658.4
Latest version!
Homo sapiens NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu65974 XM_005266660.4
Latest version!
Homo sapiens NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu65975 XM_006722421.4
Latest version!
Homo sapiens NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu65976 XM_005266663.4
Latest version!
Homo sapiens NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu65977 XM_006722424.3
Latest version!
Homo sapiens NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu65978 XM_006722425.3
Latest version!
Homo sapiens NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu104534 XM_024451130.1
Latest version!
Homo sapiens NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OHu87831 XM_017025676.1
Latest version!
Homo sapiens NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OHu87832 XM_017025677.1
Latest version!
Homo sapiens NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OHu87833 XM_017025678.2
Latest version!
Homo sapiens NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OHu87834 XM_024451132.1
Latest version!
Homo sapiens NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OHu87834 XM_017025680.2
Latest version!
Homo sapiens NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant X24, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OHu87835 XM_017025681.2
Latest version!
Homo sapiens NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant X25, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OHu51144 XM_006722426.4
Latest version!
Homo sapiens NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OHu65979 XM_011525887.3
Latest version!
Homo sapiens NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
View Old Accession Versions >>
OHu87834 XM_017025680.1 Homo sapiens neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OHu14306 NM_001144966.2 Homo sapiens neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant a, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$339.50-$475.30
$679.00
OHu87835 XM_017025681.1 Homo sapiens neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OHu14254 NM_001144971.1 Homo sapiens neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant k, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $587.30
$839.00
OHu14254 NM_001144970.2 Homo sapiens neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant c, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $587.30
$839.00
OHu65975 XM_006722421.3 Homo sapiens neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu65979 XM_011525887.2 Homo sapiens neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OHu14655 NM_001243960.1 Homo sapiens neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant i, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu14499 NM_001144969.1 Homo sapiens neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant h, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $657.30
$939.00
OHu51148 XM_006722430.3 Homo sapiens neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu51144 XM_006722426.3 Homo sapiens neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OHu87833 XM_017025678.1 Homo sapiens neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OHu14306 XM_017025679.1 Homo sapiens neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$339.50-$475.30
$679.00
OHu51146 XM_006722428.3 Homo sapiens neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu14336 NM_001144968.1 Homo sapiens neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant g, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $657.30
$939.00
OHu14742 NM_015277.5 Homo sapiens neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant d, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $657.30
$939.00
OHu14306 NM_001144965.1 Homo sapiens neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant f, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$339.50-$475.30
$679.00
OHu21352 NM_001144967.2 Homo sapiens neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant j, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$339.50-$475.30
$679.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu14306
    Clone ID Related Accession (Same CDS sequence) NM_001144965.1 , NM_001144966.2 , XM_017025679.2 , NM_001144966.3 , NM_001144964.1 , XM_017025679.1 , XM_024451129.1 , NM_001144965.2
    Accession Version NM_001144965.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2565bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-03
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY112983.1, BC032597.2 and AC090236.4. Transcript Variant: This variant (f) uses an alternate first exon, compared to variant j, which results in the use of a downstream in-frame start codon. The encoded protein (isoform 2) has a shorter N-terminus that lacks a C2 domain, compared to isoform 1. Variants a, b, and f encode the same isoform (2). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY112983.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAGCGAC CCTATACATT TAAGGACTTT CTCCTCAGAC CAAGAAGTCA TAAGTCTCGA 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCAA AAAATGGAGG TCAAGATGAA
    GAAAACAGTG ACCAGAGGGA TGACATGGAG CATGGATGGG AAGTTGTTGA CTCAAATGAC
    TCGGCTTCTC AGCACCAAGA GGAACTTCCT CCTCCTCCTC TGCCTCCCGG GTGGGAAGAA
    AAAGTGGACA ATTTAGGCCG AACTTACTAT GTCAACCACA ACAACCGGAC CACTCAGTGG
    CACAGACCAA GCCTGATGGA CGTGTCCTCG GAGTCGGACA ATAACATCAG ACAGATCAAC
    CAGGAGGCAG CACACCGGCG CTTCCGCTCC CGCAGGCACA TCAGCGAAGA CTTGGAGCCC
    GAGCCCTCGG AGGGCGGGGA TGTCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAT
    ATCGCTGGAG ACTCTCTCGG TCTGGCTCTG CCCCCACCAC CGGCCTCCCC AGGATCTCGG
    ACCAGCCCTC AGGAGCTGTC AGAGGAACTA AGCAGAAGGC TTCAGATCAC TCCAGACTCC
    AATGGGGAAC AGTTCAGCTC TTTGATTCAA AGAGAACCCT CCTCAAGGTT GAGGTCATGC
    AGTGTCACCG ACGCAGTTGC AGAACAGGGC CATCTACCAC CGCCCAGTGC CCCAGCTGGG
    AGAGCGCGTT CATCAACTGT CACGGGTGGT GAGGAACCAA CGCCATCAGT GGCCTATGTA
    CATACCACGC CGGGTCTGCC TTCAGGCTGG GAAGAAAGAA AAGATGCTAA GGGGCGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GACCTATCAT GCAGCTTGCA
    GAAGATGGTG CGTCCGGATC AGCCACAAAC AGTAACAACC ATCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CTGCCCCGCT GGAGGGTGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GACACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CTTGCCACCC
    GGCTGGGAAA TGAGGATAGC GCCAAACGGC CGGCCCTTCT TCATTGATCA TAACACAAAG
    ACTACAACCT GGGAAGATCC ACGTTTGAAA TTTCCAGTAC ATATGCGGTC AAAGACATCT
    TTAAACCCCA ATGACCTTGG CCCCCTTCCT CCTGGCTGGG AAGAAAGAAT TCACTTGGAT
    GGCCGAACGT TTTATATTGA TCATAATAGC AAAATTACTC AGTGGGAAGA CCCAAGACTG
    CAGAACCCAG CTATTACTGG TCCGGCTGTC CCTTACTCCA GAGAATTTAA GCAGAAATAT
    GACTACTTCA GGAAGAAATT AAAGAAACCT GCTGATATCC CCAATAGGTT TGAAATGAAA
    CTTCACAGAA ATAACATATT TGAAGAGTCC TATCGGAGAA TTATGTCCGT GAAAAGACCA
    GATGTCCTAA AAGCTAGACT GTGGATTGAG TTTGAATCAG AGAAAGGTCT TGACTATGGG
    GGTGTGGCCA GAGAATGGTT CTTCTTACTG TCCAAAGAGA TGTTCAACCC CTACTACGGC
    CTCTTTGAGT ACTCTGCCAC GGACAACTAC ACCCTTCAGA TCAACCCTAA TTCAGGCCTC
    TGTAATGAGG ATCATTTGTC CTACTTCACT TTTATTGGAA GAGTTGCTGG TCTGGCCGTA
    TTTCATGGGA AGCTCTTAGA TGGTTTCTTC ATTAGACCAT TTTACAAGAT GATGTTGGGA
    AAGCAGATAA CCCTGAATGA CATGGAATCT GTGGATAGTG AATATTACAA CTCTTTGAAA
    TGGATCCTGG AGAATGACCC TACTGAGCTG GACCTCATGT TCTGCATAGA CGAAGAAAAC
    TTTGGACAGA CATATCAAGT GGATTTGAAG CCCAATGGGT CAGAAATAAT GGTCACAAAT
    GAAAACAAAA GGGAATATAT CGACTTAGTC ATCCAGTGGA GATTTGTGAA CAGGGTCCAG
    AAGCAGATGA ACGCCTTCTT GGAGGGATTC ACAGAACTAC TTCCTATTGA TTTGATTAAA
    ATTTTTGATG AAAATGAGCT GGAGTTGCTC ATGTGCGGCC TCGGTGATGT GGATGTGAAT
    GACTGGAGAC AGCATTCTAT TTACAAGAAC GGCTACTGCC CAAACCACCC CGTCATTCAG
    TGGTTCTGGA AGGCTGTGCT ACTCATGGAC GCCGAAAAGC GTATCCGGTT ACTGCAGTTT
    GTCACAGGGA CATCGCGAGT ACCTATGAAT GGATTTGCCG AACTTTATGG TTCCAATGGT
    CCTCAGCTGT TTACAATAGA GCAATGGGGC AGTCCTGAGA AACTGCCCAG AGCTCACACA
    TGCTTTAATC GCCTTGACTT ACCTCCATAT GAAACCTTTG AAGATTTACG AGAGAAACTT
    CTCATGGCCG TGGAAAATGC TCAAGGATTT GAAGGGGTGG ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001138437.1
    CDS553..3117
    Translation

    Target ORF information:

    RefSeq Version NM_001144965.1
    Organism Homo sapiens(human)
    Definition Homo sapiens neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant f, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001144965.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAGCGAC CCTATACATT TAAGGACTTT CTCCTCAGAC CAAGAAGTCA TAAGTCTCGA 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCAA AAAATGGAGG TCAAGATGAA
    GAAAACAGTG ACCAGAGGGA TGACATGGAG CATGGATGGG AAGTTGTTGA CTCAAATGAC
    TCGGCTTCTC AGCACCAAGA GGAACTTCCT CCTCCTCCTC TGCCTCCCGG GTGGGAAGAA
    AAAGTGGACA ATTTAGGCCG AACTTACTAT GTCAACCACA ACAACCGGAC CACTCAGTGG
    CACAGACCAA GCCTGATGGA CGTGTCCTCG GAGTCGGACA ATAACATCAG ACAGATCAAC
    CAGGAGGCAG CACACCGGCG CTTCCGCTCC CGCAGGCACA TCAGCGAAGA CTTGGAGCCC
    GAGCCCTCGG AGGGCGGGGA TGTCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAT
    ATCGCTGGAG ACTCTCTCGG TCTGGCTCTG CCCCCACCAC CGGCCTCCCC AGGATCTCGG
    ACCAGCCCTC AGGAGCTGTC AGAGGAACTA AGCAGAAGGC TTCAGATCAC TCCAGACTCC
    AATGGGGAAC AGTTCAGCTC TTTGATTCAA AGAGAACCCT CCTCAAGGTT GAGGTCATGC
    AGTGTCACCG ACGCAGTTGC AGAACAGGGC CATCTACCAC CGCCCAGTGC CCCAGCTGGG
    AGAGCGCGTT CATCAACTGT CACGGGTGGT GAGGAACCAA CGCCATCAGT GGCCTATGTA
    CATACCACGC CGGGTCTGCC TTCAGGCTGG GAAGAAAGAA AAGATGCTAA GGGGCGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GACCTATCAT GCAGCTTGCA
    GAAGATGGTG CGTCCGGATC AGCCACAAAC AGTAACAACC ATCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CTGCCCCGCT GGAGGGTGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GACACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CTTGCCACCC
    GGCTGGGAAA TGAGGATAGC GCCAAACGGC CGGCCCTTCT TCATTGATCA TAACACAAAG
    ACTACAACCT GGGAAGATCC ACGTTTGAAA TTTCCAGTAC ATATGCGGTC AAAGACATCT
    TTAAACCCCA ATGACCTTGG CCCCCTTCCT CCTGGCTGGG AAGAAAGAAT TCACTTGGAT
    GGCCGAACGT TTTATATTGA TCATAATAGC AAAATTACTC AGTGGGAAGA CCCAAGACTG
    CAGAACCCAG CTATTACTGG TCCGGCTGTC CCTTACTCCA GAGAATTTAA GCAGAAATAT
    GACTACTTCA GGAAGAAATT AAAGAAACCT GCTGATATCC CCAATAGGTT TGAAATGAAA
    CTTCACAGAA ATAACATATT TGAAGAGTCC TATCGGAGAA TTATGTCCGT GAAAAGACCA
    GATGTCCTAA AAGCTAGACT GTGGATTGAG TTTGAATCAG AGAAAGGTCT TGACTATGGG
    GGTGTGGCCA GAGAATGGTT CTTCTTACTG TCCAAAGAGA TGTTCAACCC CTACTACGGC
    CTCTTTGAGT ACTCTGCCAC GGACAACTAC ACCCTTCAGA TCAACCCTAA TTCAGGCCTC
    TGTAATGAGG ATCATTTGTC CTACTTCACT TTTATTGGAA GAGTTGCTGG TCTGGCCGTA
    TTTCATGGGA AGCTCTTAGA TGGTTTCTTC ATTAGACCAT TTTACAAGAT GATGTTGGGA
    AAGCAGATAA CCCTGAATGA CATGGAATCT GTGGATAGTG AATATTACAA CTCTTTGAAA
    TGGATCCTGG AGAATGACCC TACTGAGCTG GACCTCATGT TCTGCATAGA CGAAGAAAAC
    TTTGGACAGA CATATCAAGT GGATTTGAAG CCCAATGGGT CAGAAATAAT GGTCACAAAT
    GAAAACAAAA GGGAATATAT CGACTTAGTC ATCCAGTGGA GATTTGTGAA CAGGGTCCAG
    AAGCAGATGA ACGCCTTCTT GGAGGGATTC ACAGAACTAC TTCCTATTGA TTTGATTAAA
    ATTTTTGATG AAAATGAGCT GGAGTTGCTC ATGTGCGGCC TCGGTGATGT GGATGTGAAT
    GACTGGAGAC AGCATTCTAT TTACAAGAAC GGCTACTGCC CAAACCACCC CGTCATTCAG
    TGGTTCTGGA AGGCTGTGCT ACTCATGGAC GCCGAAAAGC GTATCCGGTT ACTGCAGTTT
    GTCACAGGGA CATCGCGAGT ACCTATGAAT GGATTTGCCG AACTTTATGG TTCCAATGGT
    CCTCAGCTGT TTACAATAGA GCAATGGGGC AGTCCTGAGA AACTGCCCAG AGCTCACACA
    TGCTTTAATC GCCTTGACTT ACCTCCATAT GAAACCTTTG AAGATTTACG AGAGAAACTT
    CTCATGGCCG TGGAAAATGC TCAAGGATTT GAAGGGGTGG ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu14306
    Clone ID Related Accession (Same CDS sequence) NM_001144965.1 , NM_001144966.2 , XM_017025679.2 , NM_001144966.3 , NM_001144964.1 , XM_017025679.1 , XM_024451129.1 , NM_001144965.2
    Accession Version NM_001144966.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2565bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-03
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC381911.1, AF210730.1, AK300444.1 and AC090236.4. On Sep 3, 2011 this sequence version replaced NM_001144966.1. Transcript Variant: This variant (a) uses an alternate first exon, compared to variant j, which results in the use of a downstream in-frame start codon. The encoded protein (isoform 2) has a shorter N-terminus that lacks a C2 domain, compared to isoform 1. Variant a is also known as NEDD4-2A. Variants a, b, and f encode the same isoform (2). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK300444.1, AF210730.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAGCGAC CCTATACATT TAAGGACTTT CTCCTCAGAC CAAGAAGTCA TAAGTCTCGA 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCAA AAAATGGAGG TCAAGATGAA
    GAAAACAGTG ACCAGAGGGA TGACATGGAG CATGGATGGG AAGTTGTTGA CTCAAATGAC
    TCGGCTTCTC AGCACCAAGA GGAACTTCCT CCTCCTCCTC TGCCTCCCGG GTGGGAAGAA
    AAAGTGGACA ATTTAGGCCG AACTTACTAT GTCAACCACA ACAACCGGAC CACTCAGTGG
    CACAGACCAA GCCTGATGGA CGTGTCCTCG GAGTCGGACA ATAACATCAG ACAGATCAAC
    CAGGAGGCAG CACACCGGCG CTTCCGCTCC CGCAGGCACA TCAGCGAAGA CTTGGAGCCC
    GAGCCCTCGG AGGGCGGGGA TGTCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAT
    ATCGCTGGAG ACTCTCTCGG TCTGGCTCTG CCCCCACCAC CGGCCTCCCC AGGATCTCGG
    ACCAGCCCTC AGGAGCTGTC AGAGGAACTA AGCAGAAGGC TTCAGATCAC TCCAGACTCC
    AATGGGGAAC AGTTCAGCTC TTTGATTCAA AGAGAACCCT CCTCAAGGTT GAGGTCATGC
    AGTGTCACCG ACGCAGTTGC AGAACAGGGC CATCTACCAC CGCCCAGTGC CCCAGCTGGG
    AGAGCGCGTT CATCAACTGT CACGGGTGGT GAGGAACCAA CGCCATCAGT GGCCTATGTA
    CATACCACGC CGGGTCTGCC TTCAGGCTGG GAAGAAAGAA AAGATGCTAA GGGGCGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GACCTATCAT GCAGCTTGCA
    GAAGATGGTG CGTCCGGATC AGCCACAAAC AGTAACAACC ATCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CTGCCCCGCT GGAGGGTGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GACACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CTTGCCACCC
    GGCTGGGAAA TGAGGATAGC GCCAAACGGC CGGCCCTTCT TCATTGATCA TAACACAAAG
    ACTACAACCT GGGAAGATCC ACGTTTGAAA TTTCCAGTAC ATATGCGGTC AAAGACATCT
    TTAAACCCCA ATGACCTTGG CCCCCTTCCT CCTGGCTGGG AAGAAAGAAT TCACTTGGAT
    GGCCGAACGT TTTATATTGA TCATAATAGC AAAATTACTC AGTGGGAAGA CCCAAGACTG
    CAGAACCCAG CTATTACTGG TCCGGCTGTC CCTTACTCCA GAGAATTTAA GCAGAAATAT
    GACTACTTCA GGAAGAAATT AAAGAAACCT GCTGATATCC CCAATAGGTT TGAAATGAAA
    CTTCACAGAA ATAACATATT TGAAGAGTCC TATCGGAGAA TTATGTCCGT GAAAAGACCA
    GATGTCCTAA AAGCTAGACT GTGGATTGAG TTTGAATCAG AGAAAGGTCT TGACTATGGG
    GGTGTGGCCA GAGAATGGTT CTTCTTACTG TCCAAAGAGA TGTTCAACCC CTACTACGGC
    CTCTTTGAGT ACTCTGCCAC GGACAACTAC ACCCTTCAGA TCAACCCTAA TTCAGGCCTC
    TGTAATGAGG ATCATTTGTC CTACTTCACT TTTATTGGAA GAGTTGCTGG TCTGGCCGTA
    TTTCATGGGA AGCTCTTAGA TGGTTTCTTC ATTAGACCAT TTTACAAGAT GATGTTGGGA
    AAGCAGATAA CCCTGAATGA CATGGAATCT GTGGATAGTG AATATTACAA CTCTTTGAAA
    TGGATCCTGG AGAATGACCC TACTGAGCTG GACCTCATGT TCTGCATAGA CGAAGAAAAC
    TTTGGACAGA CATATCAAGT GGATTTGAAG CCCAATGGGT CAGAAATAAT GGTCACAAAT
    GAAAACAAAA GGGAATATAT CGACTTAGTC ATCCAGTGGA GATTTGTGAA CAGGGTCCAG
    AAGCAGATGA ACGCCTTCTT GGAGGGATTC ACAGAACTAC TTCCTATTGA TTTGATTAAA
    ATTTTTGATG AAAATGAGCT GGAGTTGCTC ATGTGCGGCC TCGGTGATGT GGATGTGAAT
    GACTGGAGAC AGCATTCTAT TTACAAGAAC GGCTACTGCC CAAACCACCC CGTCATTCAG
    TGGTTCTGGA AGGCTGTGCT ACTCATGGAC GCCGAAAAGC GTATCCGGTT ACTGCAGTTT
    GTCACAGGGA CATCGCGAGT ACCTATGAAT GGATTTGCCG AACTTTATGG TTCCAATGGT
    CCTCAGCTGT TTACAATAGA GCAATGGGGC AGTCCTGAGA AACTGCCCAG AGCTCACACA
    TGCTTTAATC GCCTTGACTT ACCTCCATAT GAAACCTTTG AAGATTTACG AGAGAAACTT
    CTCATGGCCG TGGAAAATGC TCAAGGATTT GAAGGGGTGG ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001138438.1
    CDS478..3042
    Translation

    Target ORF information:

    RefSeq Version NM_001144966.2
    Organism Homo sapiens(human)
    Definition Homo sapiens neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant a, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001144966.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAGCGAC CCTATACATT TAAGGACTTT CTCCTCAGAC CAAGAAGTCA TAAGTCTCGA 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCAA AAAATGGAGG TCAAGATGAA
    GAAAACAGTG ACCAGAGGGA TGACATGGAG CATGGATGGG AAGTTGTTGA CTCAAATGAC
    TCGGCTTCTC AGCACCAAGA GGAACTTCCT CCTCCTCCTC TGCCTCCCGG GTGGGAAGAA
    AAAGTGGACA ATTTAGGCCG AACTTACTAT GTCAACCACA ACAACCGGAC CACTCAGTGG
    CACAGACCAA GCCTGATGGA CGTGTCCTCG GAGTCGGACA ATAACATCAG ACAGATCAAC
    CAGGAGGCAG CACACCGGCG CTTCCGCTCC CGCAGGCACA TCAGCGAAGA CTTGGAGCCC
    GAGCCCTCGG AGGGCGGGGA TGTCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAT
    ATCGCTGGAG ACTCTCTCGG TCTGGCTCTG CCCCCACCAC CGGCCTCCCC AGGATCTCGG
    ACCAGCCCTC AGGAGCTGTC AGAGGAACTA AGCAGAAGGC TTCAGATCAC TCCAGACTCC
    AATGGGGAAC AGTTCAGCTC TTTGATTCAA AGAGAACCCT CCTCAAGGTT GAGGTCATGC
    AGTGTCACCG ACGCAGTTGC AGAACAGGGC CATCTACCAC CGCCCAGTGC CCCAGCTGGG
    AGAGCGCGTT CATCAACTGT CACGGGTGGT GAGGAACCAA CGCCATCAGT GGCCTATGTA
    CATACCACGC CGGGTCTGCC TTCAGGCTGG GAAGAAAGAA AAGATGCTAA GGGGCGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GACCTATCAT GCAGCTTGCA
    GAAGATGGTG CGTCCGGATC AGCCACAAAC AGTAACAACC ATCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CTGCCCCGCT GGAGGGTGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GACACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CTTGCCACCC
    GGCTGGGAAA TGAGGATAGC GCCAAACGGC CGGCCCTTCT TCATTGATCA TAACACAAAG
    ACTACAACCT GGGAAGATCC ACGTTTGAAA TTTCCAGTAC ATATGCGGTC AAAGACATCT
    TTAAACCCCA ATGACCTTGG CCCCCTTCCT CCTGGCTGGG AAGAAAGAAT TCACTTGGAT
    GGCCGAACGT TTTATATTGA TCATAATAGC AAAATTACTC AGTGGGAAGA CCCAAGACTG
    CAGAACCCAG CTATTACTGG TCCGGCTGTC CCTTACTCCA GAGAATTTAA GCAGAAATAT
    GACTACTTCA GGAAGAAATT AAAGAAACCT GCTGATATCC CCAATAGGTT TGAAATGAAA
    CTTCACAGAA ATAACATATT TGAAGAGTCC TATCGGAGAA TTATGTCCGT GAAAAGACCA
    GATGTCCTAA AAGCTAGACT GTGGATTGAG TTTGAATCAG AGAAAGGTCT TGACTATGGG
    GGTGTGGCCA GAGAATGGTT CTTCTTACTG TCCAAAGAGA TGTTCAACCC CTACTACGGC
    CTCTTTGAGT ACTCTGCCAC GGACAACTAC ACCCTTCAGA TCAACCCTAA TTCAGGCCTC
    TGTAATGAGG ATCATTTGTC CTACTTCACT TTTATTGGAA GAGTTGCTGG TCTGGCCGTA
    TTTCATGGGA AGCTCTTAGA TGGTTTCTTC ATTAGACCAT TTTACAAGAT GATGTTGGGA
    AAGCAGATAA CCCTGAATGA CATGGAATCT GTGGATAGTG AATATTACAA CTCTTTGAAA
    TGGATCCTGG AGAATGACCC TACTGAGCTG GACCTCATGT TCTGCATAGA CGAAGAAAAC
    TTTGGACAGA CATATCAAGT GGATTTGAAG CCCAATGGGT CAGAAATAAT GGTCACAAAT
    GAAAACAAAA GGGAATATAT CGACTTAGTC ATCCAGTGGA GATTTGTGAA CAGGGTCCAG
    AAGCAGATGA ACGCCTTCTT GGAGGGATTC ACAGAACTAC TTCCTATTGA TTTGATTAAA
    ATTTTTGATG AAAATGAGCT GGAGTTGCTC ATGTGCGGCC TCGGTGATGT GGATGTGAAT
    GACTGGAGAC AGCATTCTAT TTACAAGAAC GGCTACTGCC CAAACCACCC CGTCATTCAG
    TGGTTCTGGA AGGCTGTGCT ACTCATGGAC GCCGAAAAGC GTATCCGGTT ACTGCAGTTT
    GTCACAGGGA CATCGCGAGT ACCTATGAAT GGATTTGCCG AACTTTATGG TTCCAATGGT
    CCTCAGCTGT TTACAATAGA GCAATGGGGC AGTCCTGAGA AACTGCCCAG AGCTCACACA
    TGCTTTAATC GCCTTGACTT ACCTCCATAT GAAACCTTTG AAGATTTACG AGAGAAACTT
    CTCATGGCCG TGGAAAATGC TCAAGGATTT GAAGGGGTGG ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu14306
    Clone ID Related Accession (Same CDS sequence) NM_001144965.1 , NM_001144966.2 , XM_017025679.2 , NM_001144966.3 , NM_001144964.1 , XM_017025679.1 , XM_024451129.1 , NM_001144965.2
    Accession Version NM_001144966.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2565bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-20
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC381911.1, AF210730.1, AK300444.1 and AC090236.4. On May 31, 2019 this sequence version replaced NM_001144966.2. Transcript Variant: This variant (a) uses an alternate first exon, compared to variant j, which results in the use of a downstream in-frame start codon. The encoded protein (isoform 2) has a shorter N-terminus that lacks a C2 domain, compared to isoform 1. Variant a is also known as NEDD4-2A. Variants a, b, and f encode the same isoform (2). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK300444.1, AF210730.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAGCGAC CCTATACATT TAAGGACTTT CTCCTCAGAC CAAGAAGTCA TAAGTCTCGA 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCAA AAAATGGAGG TCAAGATGAA
    GAAAACAGTG ACCAGAGGGA TGACATGGAG CATGGATGGG AAGTTGTTGA CTCAAATGAC
    TCGGCTTCTC AGCACCAAGA GGAACTTCCT CCTCCTCCTC TGCCTCCCGG GTGGGAAGAA
    AAAGTGGACA ATTTAGGCCG AACTTACTAT GTCAACCACA ACAACCGGAC CACTCAGTGG
    CACAGACCAA GCCTGATGGA CGTGTCCTCG GAGTCGGACA ATAACATCAG ACAGATCAAC
    CAGGAGGCAG CACACCGGCG CTTCCGCTCC CGCAGGCACA TCAGCGAAGA CTTGGAGCCC
    GAGCCCTCGG AGGGCGGGGA TGTCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAT
    ATCGCTGGAG ACTCTCTCGG TCTGGCTCTG CCCCCACCAC CGGCCTCCCC AGGATCTCGG
    ACCAGCCCTC AGGAGCTGTC AGAGGAACTA AGCAGAAGGC TTCAGATCAC TCCAGACTCC
    AATGGGGAAC AGTTCAGCTC TTTGATTCAA AGAGAACCCT CCTCAAGGTT GAGGTCATGC
    AGTGTCACCG ACGCAGTTGC AGAACAGGGC CATCTACCAC CGCCCAGTGC CCCAGCTGGG
    AGAGCGCGTT CATCAACTGT CACGGGTGGT GAGGAACCAA CGCCATCAGT GGCCTATGTA
    CATACCACGC CGGGTCTGCC TTCAGGCTGG GAAGAAAGAA AAGATGCTAA GGGGCGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GACCTATCAT GCAGCTTGCA
    GAAGATGGTG CGTCCGGATC AGCCACAAAC AGTAACAACC ATCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CTGCCCCGCT GGAGGGTGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GACACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CTTGCCACCC
    GGCTGGGAAA TGAGGATAGC GCCAAACGGC CGGCCCTTCT TCATTGATCA TAACACAAAG
    ACTACAACCT GGGAAGATCC ACGTTTGAAA TTTCCAGTAC ATATGCGGTC AAAGACATCT
    TTAAACCCCA ATGACCTTGG CCCCCTTCCT CCTGGCTGGG AAGAAAGAAT TCACTTGGAT
    GGCCGAACGT TTTATATTGA TCATAATAGC AAAATTACTC AGTGGGAAGA CCCAAGACTG
    CAGAACCCAG CTATTACTGG TCCGGCTGTC CCTTACTCCA GAGAATTTAA GCAGAAATAT
    GACTACTTCA GGAAGAAATT AAAGAAACCT GCTGATATCC CCAATAGGTT TGAAATGAAA
    CTTCACAGAA ATAACATATT TGAAGAGTCC TATCGGAGAA TTATGTCCGT GAAAAGACCA
    GATGTCCTAA AAGCTAGACT GTGGATTGAG TTTGAATCAG AGAAAGGTCT TGACTATGGG
    GGTGTGGCCA GAGAATGGTT CTTCTTACTG TCCAAAGAGA TGTTCAACCC CTACTACGGC
    CTCTTTGAGT ACTCTGCCAC GGACAACTAC ACCCTTCAGA TCAACCCTAA TTCAGGCCTC
    TGTAATGAGG ATCATTTGTC CTACTTCACT TTTATTGGAA GAGTTGCTGG TCTGGCCGTA
    TTTCATGGGA AGCTCTTAGA TGGTTTCTTC ATTAGACCAT TTTACAAGAT GATGTTGGGA
    AAGCAGATAA CCCTGAATGA CATGGAATCT GTGGATAGTG AATATTACAA CTCTTTGAAA
    TGGATCCTGG AGAATGACCC TACTGAGCTG GACCTCATGT TCTGCATAGA CGAAGAAAAC
    TTTGGACAGA CATATCAAGT GGATTTGAAG CCCAATGGGT CAGAAATAAT GGTCACAAAT
    GAAAACAAAA GGGAATATAT CGACTTAGTC ATCCAGTGGA GATTTGTGAA CAGGGTCCAG
    AAGCAGATGA ACGCCTTCTT GGAGGGATTC ACAGAACTAC TTCCTATTGA TTTGATTAAA
    ATTTTTGATG AAAATGAGCT GGAGTTGCTC ATGTGCGGCC TCGGTGATGT GGATGTGAAT
    GACTGGAGAC AGCATTCTAT TTACAAGAAC GGCTACTGCC CAAACCACCC CGTCATTCAG
    TGGTTCTGGA AGGCTGTGCT ACTCATGGAC GCCGAAAAGC GTATCCGGTT ACTGCAGTTT
    GTCACAGGGA CATCGCGAGT ACCTATGAAT GGATTTGCCG AACTTTATGG TTCCAATGGT
    CCTCAGCTGT TTACAATAGA GCAATGGGGC AGTCCTGAGA AACTGCCCAG AGCTCACACA
    TGCTTTAATC GCCTTGACTT ACCTCCATAT GAAACCTTTG AAGATTTACG AGAGAAACTT
    CTCATGGCCG TGGAAAATGC TCAAGGATTT GAAGGGGTGG ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001138438.1
    CDS429..2993
    Translation

    Target ORF information:

    RefSeq Version NM_001144966.3
    Organism Homo sapiens(human)
    Definition Homo sapiens NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant a, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001144966.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAGCGAC CCTATACATT TAAGGACTTT CTCCTCAGAC CAAGAAGTCA TAAGTCTCGA 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCAA AAAATGGAGG TCAAGATGAA
    GAAAACAGTG ACCAGAGGGA TGACATGGAG CATGGATGGG AAGTTGTTGA CTCAAATGAC
    TCGGCTTCTC AGCACCAAGA GGAACTTCCT CCTCCTCCTC TGCCTCCCGG GTGGGAAGAA
    AAAGTGGACA ATTTAGGCCG AACTTACTAT GTCAACCACA ACAACCGGAC CACTCAGTGG
    CACAGACCAA GCCTGATGGA CGTGTCCTCG GAGTCGGACA ATAACATCAG ACAGATCAAC
    CAGGAGGCAG CACACCGGCG CTTCCGCTCC CGCAGGCACA TCAGCGAAGA CTTGGAGCCC
    GAGCCCTCGG AGGGCGGGGA TGTCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAT
    ATCGCTGGAG ACTCTCTCGG TCTGGCTCTG CCCCCACCAC CGGCCTCCCC AGGATCTCGG
    ACCAGCCCTC AGGAGCTGTC AGAGGAACTA AGCAGAAGGC TTCAGATCAC TCCAGACTCC
    AATGGGGAAC AGTTCAGCTC TTTGATTCAA AGAGAACCCT CCTCAAGGTT GAGGTCATGC
    AGTGTCACCG ACGCAGTTGC AGAACAGGGC CATCTACCAC CGCCCAGTGC CCCAGCTGGG
    AGAGCGCGTT CATCAACTGT CACGGGTGGT GAGGAACCAA CGCCATCAGT GGCCTATGTA
    CATACCACGC CGGGTCTGCC TTCAGGCTGG GAAGAAAGAA AAGATGCTAA GGGGCGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GACCTATCAT GCAGCTTGCA
    GAAGATGGTG CGTCCGGATC AGCCACAAAC AGTAACAACC ATCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CTGCCCCGCT GGAGGGTGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GACACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CTTGCCACCC
    GGCTGGGAAA TGAGGATAGC GCCAAACGGC CGGCCCTTCT TCATTGATCA TAACACAAAG
    ACTACAACCT GGGAAGATCC ACGTTTGAAA TTTCCAGTAC ATATGCGGTC AAAGACATCT
    TTAAACCCCA ATGACCTTGG CCCCCTTCCT CCTGGCTGGG AAGAAAGAAT TCACTTGGAT
    GGCCGAACGT TTTATATTGA TCATAATAGC AAAATTACTC AGTGGGAAGA CCCAAGACTG
    CAGAACCCAG CTATTACTGG TCCGGCTGTC CCTTACTCCA GAGAATTTAA GCAGAAATAT
    GACTACTTCA GGAAGAAATT AAAGAAACCT GCTGATATCC CCAATAGGTT TGAAATGAAA
    CTTCACAGAA ATAACATATT TGAAGAGTCC TATCGGAGAA TTATGTCCGT GAAAAGACCA
    GATGTCCTAA AAGCTAGACT GTGGATTGAG TTTGAATCAG AGAAAGGTCT TGACTATGGG
    GGTGTGGCCA GAGAATGGTT CTTCTTACTG TCCAAAGAGA TGTTCAACCC CTACTACGGC
    CTCTTTGAGT ACTCTGCCAC GGACAACTAC ACCCTTCAGA TCAACCCTAA TTCAGGCCTC
    TGTAATGAGG ATCATTTGTC CTACTTCACT TTTATTGGAA GAGTTGCTGG TCTGGCCGTA
    TTTCATGGGA AGCTCTTAGA TGGTTTCTTC ATTAGACCAT TTTACAAGAT GATGTTGGGA
    AAGCAGATAA CCCTGAATGA CATGGAATCT GTGGATAGTG AATATTACAA CTCTTTGAAA
    TGGATCCTGG AGAATGACCC TACTGAGCTG GACCTCATGT TCTGCATAGA CGAAGAAAAC
    TTTGGACAGA CATATCAAGT GGATTTGAAG CCCAATGGGT CAGAAATAAT GGTCACAAAT
    GAAAACAAAA GGGAATATAT CGACTTAGTC ATCCAGTGGA GATTTGTGAA CAGGGTCCAG
    AAGCAGATGA ACGCCTTCTT GGAGGGATTC ACAGAACTAC TTCCTATTGA TTTGATTAAA
    ATTTTTGATG AAAATGAGCT GGAGTTGCTC ATGTGCGGCC TCGGTGATGT GGATGTGAAT
    GACTGGAGAC AGCATTCTAT TTACAAGAAC GGCTACTGCC CAAACCACCC CGTCATTCAG
    TGGTTCTGGA AGGCTGTGCT ACTCATGGAC GCCGAAAAGC GTATCCGGTT ACTGCAGTTT
    GTCACAGGGA CATCGCGAGT ACCTATGAAT GGATTTGCCG AACTTTATGG TTCCAATGGT
    CCTCAGCTGT TTACAATAGA GCAATGGGGC AGTCCTGAGA AACTGCCCAG AGCTCACACA
    TGCTTTAATC GCCTTGACTT ACCTCCATAT GAAACCTTTG AAGATTTACG AGAGAAACTT
    CTCATGGCCG TGGAAAATGC TCAAGGATTT GAAGGGGTGG ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu14306
    Clone ID Related Accession (Same CDS sequence) NM_001144965.1 , NM_001144966.2 , XM_017025679.2 , NM_001144966.3 , NM_001144964.1 , XM_017025679.1 , XM_024451129.1 , NM_001144965.2
    Accession Version NM_001144964.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2565bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-20
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF385931.1, BC019345.1, BP260852.1, AF070601.1, DA759940.1, AC090236.4 and DB317092.1. Transcript Variant: This variant (b) uses an alternate first exon, compared to variant j, which results in the use of a downstream in-frame start codon. The encoded protein (isoform 2) has a shorter N-terminus that lacks a C2 domain, compared to isoform 1. Variant b is also known as NEDD4-2C7. Variants a, b, and f encode the same isoform (2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF385931.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145240, SAMEA2154665 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAGCGAC CCTATACATT TAAGGACTTT CTCCTCAGAC CAAGAAGTCA TAAGTCTCGA 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCAA AAAATGGAGG TCAAGATGAA
    GAAAACAGTG ACCAGAGGGA TGACATGGAG CATGGATGGG AAGTTGTTGA CTCAAATGAC
    TCGGCTTCTC AGCACCAAGA GGAACTTCCT CCTCCTCCTC TGCCTCCCGG GTGGGAAGAA
    AAAGTGGACA ATTTAGGCCG AACTTACTAT GTCAACCACA ACAACCGGAC CACTCAGTGG
    CACAGACCAA GCCTGATGGA CGTGTCCTCG GAGTCGGACA ATAACATCAG ACAGATCAAC
    CAGGAGGCAG CACACCGGCG CTTCCGCTCC CGCAGGCACA TCAGCGAAGA CTTGGAGCCC
    GAGCCCTCGG AGGGCGGGGA TGTCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAT
    ATCGCTGGAG ACTCTCTCGG TCTGGCTCTG CCCCCACCAC CGGCCTCCCC AGGATCTCGG
    ACCAGCCCTC AGGAGCTGTC AGAGGAACTA AGCAGAAGGC TTCAGATCAC TCCAGACTCC
    AATGGGGAAC AGTTCAGCTC TTTGATTCAA AGAGAACCCT CCTCAAGGTT GAGGTCATGC
    AGTGTCACCG ACGCAGTTGC AGAACAGGGC CATCTACCAC CGCCCAGTGC CCCAGCTGGG
    AGAGCGCGTT CATCAACTGT CACGGGTGGT GAGGAACCAA CGCCATCAGT GGCCTATGTA
    CATACCACGC CGGGTCTGCC TTCAGGCTGG GAAGAAAGAA AAGATGCTAA GGGGCGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GACCTATCAT GCAGCTTGCA
    GAAGATGGTG CGTCCGGATC AGCCACAAAC AGTAACAACC ATCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CTGCCCCGCT GGAGGGTGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GACACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CTTGCCACCC
    GGCTGGGAAA TGAGGATAGC GCCAAACGGC CGGCCCTTCT TCATTGATCA TAACACAAAG
    ACTACAACCT GGGAAGATCC ACGTTTGAAA TTTCCAGTAC ATATGCGGTC AAAGACATCT
    TTAAACCCCA ATGACCTTGG CCCCCTTCCT CCTGGCTGGG AAGAAAGAAT TCACTTGGAT
    GGCCGAACGT TTTATATTGA TCATAATAGC AAAATTACTC AGTGGGAAGA CCCAAGACTG
    CAGAACCCAG CTATTACTGG TCCGGCTGTC CCTTACTCCA GAGAATTTAA GCAGAAATAT
    GACTACTTCA GGAAGAAATT AAAGAAACCT GCTGATATCC CCAATAGGTT TGAAATGAAA
    CTTCACAGAA ATAACATATT TGAAGAGTCC TATCGGAGAA TTATGTCCGT GAAAAGACCA
    GATGTCCTAA AAGCTAGACT GTGGATTGAG TTTGAATCAG AGAAAGGTCT TGACTATGGG
    GGTGTGGCCA GAGAATGGTT CTTCTTACTG TCCAAAGAGA TGTTCAACCC CTACTACGGC
    CTCTTTGAGT ACTCTGCCAC GGACAACTAC ACCCTTCAGA TCAACCCTAA TTCAGGCCTC
    TGTAATGAGG ATCATTTGTC CTACTTCACT TTTATTGGAA GAGTTGCTGG TCTGGCCGTA
    TTTCATGGGA AGCTCTTAGA TGGTTTCTTC ATTAGACCAT TTTACAAGAT GATGTTGGGA
    AAGCAGATAA CCCTGAATGA CATGGAATCT GTGGATAGTG AATATTACAA CTCTTTGAAA
    TGGATCCTGG AGAATGACCC TACTGAGCTG GACCTCATGT TCTGCATAGA CGAAGAAAAC
    TTTGGACAGA CATATCAAGT GGATTTGAAG CCCAATGGGT CAGAAATAAT GGTCACAAAT
    GAAAACAAAA GGGAATATAT CGACTTAGTC ATCCAGTGGA GATTTGTGAA CAGGGTCCAG
    AAGCAGATGA ACGCCTTCTT GGAGGGATTC ACAGAACTAC TTCCTATTGA TTTGATTAAA
    ATTTTTGATG AAAATGAGCT GGAGTTGCTC ATGTGCGGCC TCGGTGATGT GGATGTGAAT
    GACTGGAGAC AGCATTCTAT TTACAAGAAC GGCTACTGCC CAAACCACCC CGTCATTCAG
    TGGTTCTGGA AGGCTGTGCT ACTCATGGAC GCCGAAAAGC GTATCCGGTT ACTGCAGTTT
    GTCACAGGGA CATCGCGAGT ACCTATGAAT GGATTTGCCG AACTTTATGG TTCCAATGGT
    CCTCAGCTGT TTACAATAGA GCAATGGGGC AGTCCTGAGA AACTGCCCAG AGCTCACACA
    TGCTTTAATC GCCTTGACTT ACCTCCATAT GAAACCTTTG AAGATTTACG AGAGAAACTT
    CTCATGGCCG TGGAAAATGC TCAAGGATTT GAAGGGGTGG ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001138436.1
    CDS601..3165
    Translation

    Target ORF information:

    RefSeq Version NM_001144964.1
    Organism Homo sapiens(human)
    Definition Homo sapiens NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant b, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001144964.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAGCGAC CCTATACATT TAAGGACTTT CTCCTCAGAC CAAGAAGTCA TAAGTCTCGA 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCAA AAAATGGAGG TCAAGATGAA
    GAAAACAGTG ACCAGAGGGA TGACATGGAG CATGGATGGG AAGTTGTTGA CTCAAATGAC
    TCGGCTTCTC AGCACCAAGA GGAACTTCCT CCTCCTCCTC TGCCTCCCGG GTGGGAAGAA
    AAAGTGGACA ATTTAGGCCG AACTTACTAT GTCAACCACA ACAACCGGAC CACTCAGTGG
    CACAGACCAA GCCTGATGGA CGTGTCCTCG GAGTCGGACA ATAACATCAG ACAGATCAAC
    CAGGAGGCAG CACACCGGCG CTTCCGCTCC CGCAGGCACA TCAGCGAAGA CTTGGAGCCC
    GAGCCCTCGG AGGGCGGGGA TGTCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAT
    ATCGCTGGAG ACTCTCTCGG TCTGGCTCTG CCCCCACCAC CGGCCTCCCC AGGATCTCGG
    ACCAGCCCTC AGGAGCTGTC AGAGGAACTA AGCAGAAGGC TTCAGATCAC TCCAGACTCC
    AATGGGGAAC AGTTCAGCTC TTTGATTCAA AGAGAACCCT CCTCAAGGTT GAGGTCATGC
    AGTGTCACCG ACGCAGTTGC AGAACAGGGC CATCTACCAC CGCCCAGTGC CCCAGCTGGG
    AGAGCGCGTT CATCAACTGT CACGGGTGGT GAGGAACCAA CGCCATCAGT GGCCTATGTA
    CATACCACGC CGGGTCTGCC TTCAGGCTGG GAAGAAAGAA AAGATGCTAA GGGGCGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GACCTATCAT GCAGCTTGCA
    GAAGATGGTG CGTCCGGATC AGCCACAAAC AGTAACAACC ATCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CTGCCCCGCT GGAGGGTGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GACACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CTTGCCACCC
    GGCTGGGAAA TGAGGATAGC GCCAAACGGC CGGCCCTTCT TCATTGATCA TAACACAAAG
    ACTACAACCT GGGAAGATCC ACGTTTGAAA TTTCCAGTAC ATATGCGGTC AAAGACATCT
    TTAAACCCCA ATGACCTTGG CCCCCTTCCT CCTGGCTGGG AAGAAAGAAT TCACTTGGAT
    GGCCGAACGT TTTATATTGA TCATAATAGC AAAATTACTC AGTGGGAAGA CCCAAGACTG
    CAGAACCCAG CTATTACTGG TCCGGCTGTC CCTTACTCCA GAGAATTTAA GCAGAAATAT
    GACTACTTCA GGAAGAAATT AAAGAAACCT GCTGATATCC CCAATAGGTT TGAAATGAAA
    CTTCACAGAA ATAACATATT TGAAGAGTCC TATCGGAGAA TTATGTCCGT GAAAAGACCA
    GATGTCCTAA AAGCTAGACT GTGGATTGAG TTTGAATCAG AGAAAGGTCT TGACTATGGG
    GGTGTGGCCA GAGAATGGTT CTTCTTACTG TCCAAAGAGA TGTTCAACCC CTACTACGGC
    CTCTTTGAGT ACTCTGCCAC GGACAACTAC ACCCTTCAGA TCAACCCTAA TTCAGGCCTC
    TGTAATGAGG ATCATTTGTC CTACTTCACT TTTATTGGAA GAGTTGCTGG TCTGGCCGTA
    TTTCATGGGA AGCTCTTAGA TGGTTTCTTC ATTAGACCAT TTTACAAGAT GATGTTGGGA
    AAGCAGATAA CCCTGAATGA CATGGAATCT GTGGATAGTG AATATTACAA CTCTTTGAAA
    TGGATCCTGG AGAATGACCC TACTGAGCTG GACCTCATGT TCTGCATAGA CGAAGAAAAC
    TTTGGACAGA CATATCAAGT GGATTTGAAG CCCAATGGGT CAGAAATAAT GGTCACAAAT
    GAAAACAAAA GGGAATATAT CGACTTAGTC ATCCAGTGGA GATTTGTGAA CAGGGTCCAG
    AAGCAGATGA ACGCCTTCTT GGAGGGATTC ACAGAACTAC TTCCTATTGA TTTGATTAAA
    ATTTTTGATG AAAATGAGCT GGAGTTGCTC ATGTGCGGCC TCGGTGATGT GGATGTGAAT
    GACTGGAGAC AGCATTCTAT TTACAAGAAC GGCTACTGCC CAAACCACCC CGTCATTCAG
    TGGTTCTGGA AGGCTGTGCT ACTCATGGAC GCCGAAAAGC GTATCCGGTT ACTGCAGTTT
    GTCACAGGGA CATCGCGAGT ACCTATGAAT GGATTTGCCG AACTTTATGG TTCCAATGGT
    CCTCAGCTGT TTACAATAGA GCAATGGGGC AGTCCTGAGA AACTGCCCAG AGCTCACACA
    TGCTTTAATC GCCTTGACTT ACCTCCATAT GAAACCTTTG AAGATTTACG AGAGAAACTT
    CTCATGGCCG TGGAAAATGC TCAAGGATTT GAAGGGGTGG ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu14306
    Clone ID Related Accession (Same CDS sequence) NM_001144965.1 , NM_001144966.2 , XM_017025679.2 , NM_001144966.3 , NM_001144964.1 , XM_017025679.1 , XM_024451129.1 , NM_001144965.2
    Accession Version NM_001144965.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2565bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-20
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC107896.7, AY112983.1, BC032597.2 and AC090236.4. On May 31, 2019 this sequence version replaced NM_001144965.1. Transcript Variant: This variant (f) uses an alternate first exon, compared to variant j, which results in the use of a downstream in-frame start codon. The encoded protein (isoform 2) has a shorter N-terminus that lacks a C2 domain, compared to isoform 1. Variants a, b, and f encode the same isoform (2). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY112983.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAGCGAC CCTATACATT TAAGGACTTT CTCCTCAGAC CAAGAAGTCA TAAGTCTCGA 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCAA AAAATGGAGG TCAAGATGAA
    GAAAACAGTG ACCAGAGGGA TGACATGGAG CATGGATGGG AAGTTGTTGA CTCAAATGAC
    TCGGCTTCTC AGCACCAAGA GGAACTTCCT CCTCCTCCTC TGCCTCCCGG GTGGGAAGAA
    AAAGTGGACA ATTTAGGCCG AACTTACTAT GTCAACCACA ACAACCGGAC CACTCAGTGG
    CACAGACCAA GCCTGATGGA CGTGTCCTCG GAGTCGGACA ATAACATCAG ACAGATCAAC
    CAGGAGGCAG CACACCGGCG CTTCCGCTCC CGCAGGCACA TCAGCGAAGA CTTGGAGCCC
    GAGCCCTCGG AGGGCGGGGA TGTCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAT
    ATCGCTGGAG ACTCTCTCGG TCTGGCTCTG CCCCCACCAC CGGCCTCCCC AGGATCTCGG
    ACCAGCCCTC AGGAGCTGTC AGAGGAACTA AGCAGAAGGC TTCAGATCAC TCCAGACTCC
    AATGGGGAAC AGTTCAGCTC TTTGATTCAA AGAGAACCCT CCTCAAGGTT GAGGTCATGC
    AGTGTCACCG ACGCAGTTGC AGAACAGGGC CATCTACCAC CGCCCAGTGC CCCAGCTGGG
    AGAGCGCGTT CATCAACTGT CACGGGTGGT GAGGAACCAA CGCCATCAGT GGCCTATGTA
    CATACCACGC CGGGTCTGCC TTCAGGCTGG GAAGAAAGAA AAGATGCTAA GGGGCGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GACCTATCAT GCAGCTTGCA
    GAAGATGGTG CGTCCGGATC AGCCACAAAC AGTAACAACC ATCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CTGCCCCGCT GGAGGGTGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GACACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CTTGCCACCC
    GGCTGGGAAA TGAGGATAGC GCCAAACGGC CGGCCCTTCT TCATTGATCA TAACACAAAG
    ACTACAACCT GGGAAGATCC ACGTTTGAAA TTTCCAGTAC ATATGCGGTC AAAGACATCT
    TTAAACCCCA ATGACCTTGG CCCCCTTCCT CCTGGCTGGG AAGAAAGAAT TCACTTGGAT
    GGCCGAACGT TTTATATTGA TCATAATAGC AAAATTACTC AGTGGGAAGA CCCAAGACTG
    CAGAACCCAG CTATTACTGG TCCGGCTGTC CCTTACTCCA GAGAATTTAA GCAGAAATAT
    GACTACTTCA GGAAGAAATT AAAGAAACCT GCTGATATCC CCAATAGGTT TGAAATGAAA
    CTTCACAGAA ATAACATATT TGAAGAGTCC TATCGGAGAA TTATGTCCGT GAAAAGACCA
    GATGTCCTAA AAGCTAGACT GTGGATTGAG TTTGAATCAG AGAAAGGTCT TGACTATGGG
    GGTGTGGCCA GAGAATGGTT CTTCTTACTG TCCAAAGAGA TGTTCAACCC CTACTACGGC
    CTCTTTGAGT ACTCTGCCAC GGACAACTAC ACCCTTCAGA TCAACCCTAA TTCAGGCCTC
    TGTAATGAGG ATCATTTGTC CTACTTCACT TTTATTGGAA GAGTTGCTGG TCTGGCCGTA
    TTTCATGGGA AGCTCTTAGA TGGTTTCTTC ATTAGACCAT TTTACAAGAT GATGTTGGGA
    AAGCAGATAA CCCTGAATGA CATGGAATCT GTGGATAGTG AATATTACAA CTCTTTGAAA
    TGGATCCTGG AGAATGACCC TACTGAGCTG GACCTCATGT TCTGCATAGA CGAAGAAAAC
    TTTGGACAGA CATATCAAGT GGATTTGAAG CCCAATGGGT CAGAAATAAT GGTCACAAAT
    GAAAACAAAA GGGAATATAT CGACTTAGTC ATCCAGTGGA GATTTGTGAA CAGGGTCCAG
    AAGCAGATGA ACGCCTTCTT GGAGGGATTC ACAGAACTAC TTCCTATTGA TTTGATTAAA
    ATTTTTGATG AAAATGAGCT GGAGTTGCTC ATGTGCGGCC TCGGTGATGT GGATGTGAAT
    GACTGGAGAC AGCATTCTAT TTACAAGAAC GGCTACTGCC CAAACCACCC CGTCATTCAG
    TGGTTCTGGA AGGCTGTGCT ACTCATGGAC GCCGAAAAGC GTATCCGGTT ACTGCAGTTT
    GTCACAGGGA CATCGCGAGT ACCTATGAAT GGATTTGCCG AACTTTATGG TTCCAATGGT
    CCTCAGCTGT TTACAATAGA GCAATGGGGC AGTCCTGAGA AACTGCCCAG AGCTCACACA
    TGCTTTAATC GCCTTGACTT ACCTCCATAT GAAACCTTTG AAGATTTACG AGAGAAACTT
    CTCATGGCCG TGGAAAATGC TCAAGGATTT GAAGGGGTGG ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001138437.1
    CDS566..3130
    Translation

    Target ORF information:

    RefSeq Version NM_001144965.2
    Organism Homo sapiens(human)
    Definition Homo sapiens NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant f, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001144965.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAGCGAC CCTATACATT TAAGGACTTT CTCCTCAGAC CAAGAAGTCA TAAGTCTCGA 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCAA AAAATGGAGG TCAAGATGAA
    GAAAACAGTG ACCAGAGGGA TGACATGGAG CATGGATGGG AAGTTGTTGA CTCAAATGAC
    TCGGCTTCTC AGCACCAAGA GGAACTTCCT CCTCCTCCTC TGCCTCCCGG GTGGGAAGAA
    AAAGTGGACA ATTTAGGCCG AACTTACTAT GTCAACCACA ACAACCGGAC CACTCAGTGG
    CACAGACCAA GCCTGATGGA CGTGTCCTCG GAGTCGGACA ATAACATCAG ACAGATCAAC
    CAGGAGGCAG CACACCGGCG CTTCCGCTCC CGCAGGCACA TCAGCGAAGA CTTGGAGCCC
    GAGCCCTCGG AGGGCGGGGA TGTCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAT
    ATCGCTGGAG ACTCTCTCGG TCTGGCTCTG CCCCCACCAC CGGCCTCCCC AGGATCTCGG
    ACCAGCCCTC AGGAGCTGTC AGAGGAACTA AGCAGAAGGC TTCAGATCAC TCCAGACTCC
    AATGGGGAAC AGTTCAGCTC TTTGATTCAA AGAGAACCCT CCTCAAGGTT GAGGTCATGC
    AGTGTCACCG ACGCAGTTGC AGAACAGGGC CATCTACCAC CGCCCAGTGC CCCAGCTGGG
    AGAGCGCGTT CATCAACTGT CACGGGTGGT GAGGAACCAA CGCCATCAGT GGCCTATGTA
    CATACCACGC CGGGTCTGCC TTCAGGCTGG GAAGAAAGAA AAGATGCTAA GGGGCGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GACCTATCAT GCAGCTTGCA
    GAAGATGGTG CGTCCGGATC AGCCACAAAC AGTAACAACC ATCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CTGCCCCGCT GGAGGGTGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GACACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CTTGCCACCC
    GGCTGGGAAA TGAGGATAGC GCCAAACGGC CGGCCCTTCT TCATTGATCA TAACACAAAG
    ACTACAACCT GGGAAGATCC ACGTTTGAAA TTTCCAGTAC ATATGCGGTC AAAGACATCT
    TTAAACCCCA ATGACCTTGG CCCCCTTCCT CCTGGCTGGG AAGAAAGAAT TCACTTGGAT
    GGCCGAACGT TTTATATTGA TCATAATAGC AAAATTACTC AGTGGGAAGA CCCAAGACTG
    CAGAACCCAG CTATTACTGG TCCGGCTGTC CCTTACTCCA GAGAATTTAA GCAGAAATAT
    GACTACTTCA GGAAGAAATT AAAGAAACCT GCTGATATCC CCAATAGGTT TGAAATGAAA
    CTTCACAGAA ATAACATATT TGAAGAGTCC TATCGGAGAA TTATGTCCGT GAAAAGACCA
    GATGTCCTAA AAGCTAGACT GTGGATTGAG TTTGAATCAG AGAAAGGTCT TGACTATGGG
    GGTGTGGCCA GAGAATGGTT CTTCTTACTG TCCAAAGAGA TGTTCAACCC CTACTACGGC
    CTCTTTGAGT ACTCTGCCAC GGACAACTAC ACCCTTCAGA TCAACCCTAA TTCAGGCCTC
    TGTAATGAGG ATCATTTGTC CTACTTCACT TTTATTGGAA GAGTTGCTGG TCTGGCCGTA
    TTTCATGGGA AGCTCTTAGA TGGTTTCTTC ATTAGACCAT TTTACAAGAT GATGTTGGGA
    AAGCAGATAA CCCTGAATGA CATGGAATCT GTGGATAGTG AATATTACAA CTCTTTGAAA
    TGGATCCTGG AGAATGACCC TACTGAGCTG GACCTCATGT TCTGCATAGA CGAAGAAAAC
    TTTGGACAGA CATATCAAGT GGATTTGAAG CCCAATGGGT CAGAAATAAT GGTCACAAAT
    GAAAACAAAA GGGAATATAT CGACTTAGTC ATCCAGTGGA GATTTGTGAA CAGGGTCCAG
    AAGCAGATGA ACGCCTTCTT GGAGGGATTC ACAGAACTAC TTCCTATTGA TTTGATTAAA
    ATTTTTGATG AAAATGAGCT GGAGTTGCTC ATGTGCGGCC TCGGTGATGT GGATGTGAAT
    GACTGGAGAC AGCATTCTAT TTACAAGAAC GGCTACTGCC CAAACCACCC CGTCATTCAG
    TGGTTCTGGA AGGCTGTGCT ACTCATGGAC GCCGAAAAGC GTATCCGGTT ACTGCAGTTT
    GTCACAGGGA CATCGCGAGT ACCTATGAAT GGATTTGCCG AACTTTATGG TTCCAATGGT
    CCTCAGCTGT TTACAATAGA GCAATGGGGC AGTCCTGAGA AACTGCCCAG AGCTCACACA
    TGCTTTAATC GCCTTGACTT ACCTCCATAT GAAACCTTTG AAGATTTACG AGAGAAACTT
    CTCATGGCCG TGGAAAATGC TCAAGGATTT GAAGGGGTGG ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu21352
    Clone ID Related Accession (Same CDS sequence) NM_001144967.2 , NM_001144967.3
    Accession Version NM_001144967.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2928bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-12-31
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC107896.7, AY312514.1, AC090236.4 and BQ215431.1. This sequence is a reference standard in the RefSeqGene project. On Sep 3, 2011 this sequence version replaced NM_001144967.1. Transcript Variant: This variant (j) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY312514.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGCGACCG GGCTCGGGGA GCCGGTCTAT GGACTTTCCG AAGACGAGGG AGAGTCCCGT 
    ATTCTCAGAG TAAAAGTTGT TTCTGGAATT GATCTCGCCA AAAAGGACAT CTTTGGAGCC
    AGTGATCCGT ATGTGAAACT TTCATTGTAC GTAGCGGATG AGAATAGAGA ACTTGCTTTG
    GTCCAGACAA AAACAATTAA AAAGACACTG AACCCAAAAT GGAATGAAGA ATTTTATTTC
    AGGGTAAACC CATCTAATCA CAGACTCCTA TTTGAAGTAT TTGACGAAAA TAGACTGACA
    CGAGACGACT TCCTGGGCCA GGTGGACGTG CCCCTTAGTC ACCTTCCGAC AGAAGATCCA
    ACCATGGAGC GACCCTATAC ATTTAAGGAC TTTCTCCTCA GACCAAGAAG TCATAAGTCT
    CGAGTTAAGG GATTTTTGCG ATTGAAAATG GCCTATATGC CAAAAAATGG AGGTCAAGAT
    GAAGAAAACA GTGACCAGAG GGATGACATG GAGCATGGAT GGGAAGTTGT TGACTCAAAT
    GACTCGGCTT CTCAGCACCA AGAGGAACTT CCTCCTCCTC CTCTGCCTCC CGGGTGGGAA
    GAAAAAGTGG ACAATTTAGG CCGAACTTAC TATGTCAACC ACAACAACCG GACCACTCAG
    TGGCACAGAC CAAGCCTGAT GGACGTGTCC TCGGAGTCGG ACAATAACAT CAGACAGATC
    AACCAGGAGG CAGCACACCG GCGCTTCCGC TCCCGCAGGC ACATCAGCGA AGACTTGGAG
    CCCGAGCCCT CGGAGGGCGG GGATGTCCCC GAGCCTTGGG AGACCATTTC AGAGGAAGTG
    AATATCGCTG GAGACTCTCT CGGTCTGGCT CTGCCCCCAC CACCGGCCTC CCCAGGATCT
    CGGACCAGCC CTCAGGAGCT GTCAGAGGAA CTAAGCAGAA GGCTTCAGAT CACTCCAGAC
    TCCAATGGGG AACAGTTCAG CTCTTTGATT CAAAGAGAAC CCTCCTCAAG GTTGAGGTCA
    TGCAGTGTCA CCGACGCAGT TGCAGAACAG GGCCATCTAC CACCGCCCAG TGCCCCAGCT
    GGGAGAGCGC GTTCATCAAC TGTCACGGGT GGTGAGGAAC CAACGCCATC AGTGGCCTAT
    GTACATACCA CGCCGGGTCT GCCTTCAGGC TGGGAAGAAA GAAAAGATGC TAAGGGGCGC
    ACATACTATG TCAATCATAA CAATCGAACC ACAACTTGGA CTCGACCTAT CATGCAGCTT
    GCAGAAGATG GTGCGTCCGG ATCAGCCACA AACAGTAACA ACCATCTAAT CGAGCCTCAG
    ATCCGCCGGC CTCGTAGCCT CAGCTCGCCA ACAGTAACTT TATCTGCCCC GCTGGAGGGT
    GCCAAGGACT CACCCGTACG TCGGGCTGTG AAAGACACCC TTTCCAACCC ACAGTCCCCA
    CAGCCATCAC CTTACAACTC CCCCAAACCA CAACACAAAG TCACACAGAG CTTCTTGCCA
    CCCGGCTGGG AAATGAGGAT AGCGCCAAAC GGCCGGCCCT TCTTCATTGA TCATAACACA
    AAGACTACAA CCTGGGAAGA TCCACGTTTG AAATTTCCAG TACATATGCG GTCAAAGACA
    TCTTTAAACC CCAATGACCT TGGCCCCCTT CCTCCTGGCT GGGAAGAAAG AATTCACTTG
    GATGGCCGAA CGTTTTATAT TGATCATAAT AGCAAAATTA CTCAGTGGGA AGACCCAAGA
    CTGCAGAACC CAGCTATTAC TGGTCCGGCT GTCCCTTACT CCAGAGAATT TAAGCAGAAA
    TATGACTACT TCAGGAAGAA ATTAAAGAAA CCTGCTGATA TCCCCAATAG GTTTGAAATG
    AAACTTCACA GAAATAACAT ATTTGAAGAG TCCTATCGGA GAATTATGTC CGTGAAAAGA
    CCAGATGTCC TAAAAGCTAG ACTGTGGATT GAGTTTGAAT CAGAGAAAGG TCTTGACTAT
    GGGGGTGTGG CCAGAGAATG GTTCTTCTTA CTGTCCAAAG AGATGTTCAA CCCCTACTAC
    GGCCTCTTTG AGTACTCTGC CACGGACAAC TACACCCTTC AGATCAACCC TAATTCAGGC
    CTCTGTAATG AGGATCATTT GTCCTACTTC ACTTTTATTG GAAGAGTTGC TGGTCTGGCC
    GTATTTCATG GGAAGCTCTT AGATGGTTTC TTCATTAGAC CATTTTACAA GATGATGTTG
    GGAAAGCAGA TAACCCTGAA TGACATGGAA TCTGTGGATA GTGAATATTA CAACTCTTTG
    AAATGGATCC TGGAGAATGA CCCTACTGAG CTGGACCTCA TGTTCTGCAT AGACGAAGAA
    AACTTTGGAC AGACATATCA AGTGGATTTG AAGCCCAATG GGTCAGAAAT AATGGTCACA
    AATGAAAACA AAAGGGAATA TATCGACTTA GTCATCCAGT GGAGATTTGT GAACAGGGTC
    CAGAAGCAGA TGAACGCCTT CTTGGAGGGA TTCACAGAAC TACTTCCTAT TGATTTGATT
    AAAATTTTTG ATGAAAATGA GCTGGAGTTG CTCATGTGCG GCCTCGGTGA TGTGGATGTG
    AATGACTGGA GACAGCATTC TATTTACAAG AACGGCTACT GCCCAAACCA CCCCGTCATT
    CAGTGGTTCT GGAAGGCTGT GCTACTCATG GACGCCGAAA AGCGTATCCG GTTACTGCAG
    TTTGTCACAG GGACATCGCG AGTACCTATG AATGGATTTG CCGAACTTTA TGGTTCCAAT
    GGTCCTCAGC TGTTTACAAT AGAGCAATGG GGCAGTCCTG AGAAACTGCC CAGAGCTCAC
    ACATGCTTTA ATCGCCTTGA CTTACCTCCA TATGAAACCT TTGAAGATTT ACGAGAGAAA
    CTTCTCATGG CCGTGGAAAA TGCTCAAGGA TTTGAAGGGG TGGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001138439.1
    CDS284..3211
    Translation

    Target ORF information:

    RefSeq Version NM_001144967.2
    Organism Homo sapiens(human)
    Definition Homo sapiens neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant j, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001144967.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGCGACCG GGCTCGGGGA GCCGGTCTAT GGACTTTCCG AAGACGAGGG AGAGTCCCGT 
    ATTCTCAGAG TAAAAGTTGT TTCTGGAATT GATCTCGCCA AAAAGGACAT CTTTGGAGCC
    AGTGATCCGT ATGTGAAACT TTCATTGTAC GTAGCGGATG AGAATAGAGA ACTTGCTTTG
    GTCCAGACAA AAACAATTAA AAAGACACTG AACCCAAAAT GGAATGAAGA ATTTTATTTC
    AGGGTAAACC CATCTAATCA CAGACTCCTA TTTGAAGTAT TTGACGAAAA TAGACTGACA
    CGAGACGACT TCCTGGGCCA GGTGGACGTG CCCCTTAGTC ACCTTCCGAC AGAAGATCCA
    ACCATGGAGC GACCCTATAC ATTTAAGGAC TTTCTCCTCA GACCAAGAAG TCATAAGTCT
    CGAGTTAAGG GATTTTTGCG ATTGAAAATG GCCTATATGC CAAAAAATGG AGGTCAAGAT
    GAAGAAAACA GTGACCAGAG GGATGACATG GAGCATGGAT GGGAAGTTGT TGACTCAAAT
    GACTCGGCTT CTCAGCACCA AGAGGAACTT CCTCCTCCTC CTCTGCCTCC CGGGTGGGAA
    GAAAAAGTGG ACAATTTAGG CCGAACTTAC TATGTCAACC ACAACAACCG GACCACTCAG
    TGGCACAGAC CAAGCCTGAT GGACGTGTCC TCGGAGTCGG ACAATAACAT CAGACAGATC
    AACCAGGAGG CAGCACACCG GCGCTTCCGC TCCCGCAGGC ACATCAGCGA AGACTTGGAG
    CCCGAGCCCT CGGAGGGCGG GGATGTCCCC GAGCCTTGGG AGACCATTTC AGAGGAAGTG
    AATATCGCTG GAGACTCTCT CGGTCTGGCT CTGCCCCCAC CACCGGCCTC CCCAGGATCT
    CGGACCAGCC CTCAGGAGCT GTCAGAGGAA CTAAGCAGAA GGCTTCAGAT CACTCCAGAC
    TCCAATGGGG AACAGTTCAG CTCTTTGATT CAAAGAGAAC CCTCCTCAAG GTTGAGGTCA
    TGCAGTGTCA CCGACGCAGT TGCAGAACAG GGCCATCTAC CACCGCCCAG TGCCCCAGCT
    GGGAGAGCGC GTTCATCAAC TGTCACGGGT GGTGAGGAAC CAACGCCATC AGTGGCCTAT
    GTACATACCA CGCCGGGTCT GCCTTCAGGC TGGGAAGAAA GAAAAGATGC TAAGGGGCGC
    ACATACTATG TCAATCATAA CAATCGAACC ACAACTTGGA CTCGACCTAT CATGCAGCTT
    GCAGAAGATG GTGCGTCCGG ATCAGCCACA AACAGTAACA ACCATCTAAT CGAGCCTCAG
    ATCCGCCGGC CTCGTAGCCT CAGCTCGCCA ACAGTAACTT TATCTGCCCC GCTGGAGGGT
    GCCAAGGACT CACCCGTACG TCGGGCTGTG AAAGACACCC TTTCCAACCC ACAGTCCCCA
    CAGCCATCAC CTTACAACTC CCCCAAACCA CAACACAAAG TCACACAGAG CTTCTTGCCA
    CCCGGCTGGG AAATGAGGAT AGCGCCAAAC GGCCGGCCCT TCTTCATTGA TCATAACACA
    AAGACTACAA CCTGGGAAGA TCCACGTTTG AAATTTCCAG TACATATGCG GTCAAAGACA
    TCTTTAAACC CCAATGACCT TGGCCCCCTT CCTCCTGGCT GGGAAGAAAG AATTCACTTG
    GATGGCCGAA CGTTTTATAT TGATCATAAT AGCAAAATTA CTCAGTGGGA AGACCCAAGA
    CTGCAGAACC CAGCTATTAC TGGTCCGGCT GTCCCTTACT CCAGAGAATT TAAGCAGAAA
    TATGACTACT TCAGGAAGAA ATTAAAGAAA CCTGCTGATA TCCCCAATAG GTTTGAAATG
    AAACTTCACA GAAATAACAT ATTTGAAGAG TCCTATCGGA GAATTATGTC CGTGAAAAGA
    CCAGATGTCC TAAAAGCTAG ACTGTGGATT GAGTTTGAAT CAGAGAAAGG TCTTGACTAT
    GGGGGTGTGG CCAGAGAATG GTTCTTCTTA CTGTCCAAAG AGATGTTCAA CCCCTACTAC
    GGCCTCTTTG AGTACTCTGC CACGGACAAC TACACCCTTC AGATCAACCC TAATTCAGGC
    CTCTGTAATG AGGATCATTT GTCCTACTTC ACTTTTATTG GAAGAGTTGC TGGTCTGGCC
    GTATTTCATG GGAAGCTCTT AGATGGTTTC TTCATTAGAC CATTTTACAA GATGATGTTG
    GGAAAGCAGA TAACCCTGAA TGACATGGAA TCTGTGGATA GTGAATATTA CAACTCTTTG
    AAATGGATCC TGGAGAATGA CCCTACTGAG CTGGACCTCA TGTTCTGCAT AGACGAAGAA
    AACTTTGGAC AGACATATCA AGTGGATTTG AAGCCCAATG GGTCAGAAAT AATGGTCACA
    AATGAAAACA AAAGGGAATA TATCGACTTA GTCATCCAGT GGAGATTTGT GAACAGGGTC
    CAGAAGCAGA TGAACGCCTT CTTGGAGGGA TTCACAGAAC TACTTCCTAT TGATTTGATT
    AAAATTTTTG ATGAAAATGA GCTGGAGTTG CTCATGTGCG GCCTCGGTGA TGTGGATGTG
    AATGACTGGA GACAGCATTC TATTTACAAG AACGGCTACT GCCCAAACCA CCCCGTCATT
    CAGTGGTTCT GGAAGGCTGT GCTACTCATG GACGCCGAAA AGCGTATCCG GTTACTGCAG
    TTTGTCACAG GGACATCGCG AGTACCTATG AATGGATTTG CCGAACTTTA TGGTTCCAAT
    GGTCCTCAGC TGTTTACAAT AGAGCAATGG GGCAGTCCTG AGAAACTGCC CAGAGCTCAC
    ACATGCTTTA ATCGCCTTGA CTTACCTCCA TATGAAACCT TTGAAGATTT ACGAGAGAAA
    CTTCTCATGG CCGTGGAAAA TGCTCAAGGA TTTGAAGGGG TGGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu21352
    Clone ID Related Accession (Same CDS sequence) NM_001144967.2 , NM_001144967.3
    Accession Version NM_001144967.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2928bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-01
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC107896.7, AY312514.1, AC090236.4 and BQ215431.1. This sequence is a reference standard in the RefSeqGene project. On Nov 24, 2018 this sequence version replaced NM_001144967.2. Transcript Variant: This variant (j) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000400345.8/ ENSP00000383199.2 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGCGACCG GGCTCGGGGA GCCGGTCTAT GGACTTTCCG AAGACGAGGG AGAGTCCCGT 
    ATTCTCAGAG TAAAAGTTGT TTCTGGAATT GATCTCGCCA AAAAGGACAT CTTTGGAGCC
    AGTGATCCGT ATGTGAAACT TTCATTGTAC GTAGCGGATG AGAATAGAGA ACTTGCTTTG
    GTCCAGACAA AAACAATTAA AAAGACACTG AACCCAAAAT GGAATGAAGA ATTTTATTTC
    AGGGTAAACC CATCTAATCA CAGACTCCTA TTTGAAGTAT TTGACGAAAA TAGACTGACA
    CGAGACGACT TCCTGGGCCA GGTGGACGTG CCCCTTAGTC ACCTTCCGAC AGAAGATCCA
    ACCATGGAGC GACCCTATAC ATTTAAGGAC TTTCTCCTCA GACCAAGAAG TCATAAGTCT
    CGAGTTAAGG GATTTTTGCG ATTGAAAATG GCCTATATGC CAAAAAATGG AGGTCAAGAT
    GAAGAAAACA GTGACCAGAG GGATGACATG GAGCATGGAT GGGAAGTTGT TGACTCAAAT
    GACTCGGCTT CTCAGCACCA AGAGGAACTT CCTCCTCCTC CTCTGCCTCC CGGGTGGGAA
    GAAAAAGTGG ACAATTTAGG CCGAACTTAC TATGTCAACC ACAACAACCG GACCACTCAG
    TGGCACAGAC CAAGCCTGAT GGACGTGTCC TCGGAGTCGG ACAATAACAT CAGACAGATC
    AACCAGGAGG CAGCACACCG GCGCTTCCGC TCCCGCAGGC ACATCAGCGA AGACTTGGAG
    CCCGAGCCCT CGGAGGGCGG GGATGTCCCC GAGCCTTGGG AGACCATTTC AGAGGAAGTG
    AATATCGCTG GAGACTCTCT CGGTCTGGCT CTGCCCCCAC CACCGGCCTC CCCAGGATCT
    CGGACCAGCC CTCAGGAGCT GTCAGAGGAA CTAAGCAGAA GGCTTCAGAT CACTCCAGAC
    TCCAATGGGG AACAGTTCAG CTCTTTGATT CAAAGAGAAC CCTCCTCAAG GTTGAGGTCA
    TGCAGTGTCA CCGACGCAGT TGCAGAACAG GGCCATCTAC CACCGCCCAG TGCCCCAGCT
    GGGAGAGCGC GTTCATCAAC TGTCACGGGT GGTGAGGAAC CAACGCCATC AGTGGCCTAT
    GTACATACCA CGCCGGGTCT GCCTTCAGGC TGGGAAGAAA GAAAAGATGC TAAGGGGCGC
    ACATACTATG TCAATCATAA CAATCGAACC ACAACTTGGA CTCGACCTAT CATGCAGCTT
    GCAGAAGATG GTGCGTCCGG ATCAGCCACA AACAGTAACA ACCATCTAAT CGAGCCTCAG
    ATCCGCCGGC CTCGTAGCCT CAGCTCGCCA ACAGTAACTT TATCTGCCCC GCTGGAGGGT
    GCCAAGGACT CACCCGTACG TCGGGCTGTG AAAGACACCC TTTCCAACCC ACAGTCCCCA
    CAGCCATCAC CTTACAACTC CCCCAAACCA CAACACAAAG TCACACAGAG CTTCTTGCCA
    CCCGGCTGGG AAATGAGGAT AGCGCCAAAC GGCCGGCCCT TCTTCATTGA TCATAACACA
    AAGACTACAA CCTGGGAAGA TCCACGTTTG AAATTTCCAG TACATATGCG GTCAAAGACA
    TCTTTAAACC CCAATGACCT TGGCCCCCTT CCTCCTGGCT GGGAAGAAAG AATTCACTTG
    GATGGCCGAA CGTTTTATAT TGATCATAAT AGCAAAATTA CTCAGTGGGA AGACCCAAGA
    CTGCAGAACC CAGCTATTAC TGGTCCGGCT GTCCCTTACT CCAGAGAATT TAAGCAGAAA
    TATGACTACT TCAGGAAGAA ATTAAAGAAA CCTGCTGATA TCCCCAATAG GTTTGAAATG
    AAACTTCACA GAAATAACAT ATTTGAAGAG TCCTATCGGA GAATTATGTC CGTGAAAAGA
    CCAGATGTCC TAAAAGCTAG ACTGTGGATT GAGTTTGAAT CAGAGAAAGG TCTTGACTAT
    GGGGGTGTGG CCAGAGAATG GTTCTTCTTA CTGTCCAAAG AGATGTTCAA CCCCTACTAC
    GGCCTCTTTG AGTACTCTGC CACGGACAAC TACACCCTTC AGATCAACCC TAATTCAGGC
    CTCTGTAATG AGGATCATTT GTCCTACTTC ACTTTTATTG GAAGAGTTGC TGGTCTGGCC
    GTATTTCATG GGAAGCTCTT AGATGGTTTC TTCATTAGAC CATTTTACAA GATGATGTTG
    GGAAAGCAGA TAACCCTGAA TGACATGGAA TCTGTGGATA GTGAATATTA CAACTCTTTG
    AAATGGATCC TGGAGAATGA CCCTACTGAG CTGGACCTCA TGTTCTGCAT AGACGAAGAA
    AACTTTGGAC AGACATATCA AGTGGATTTG AAGCCCAATG GGTCAGAAAT AATGGTCACA
    AATGAAAACA AAAGGGAATA TATCGACTTA GTCATCCAGT GGAGATTTGT GAACAGGGTC
    CAGAAGCAGA TGAACGCCTT CTTGGAGGGA TTCACAGAAC TACTTCCTAT TGATTTGATT
    AAAATTTTTG ATGAAAATGA GCTGGAGTTG CTCATGTGCG GCCTCGGTGA TGTGGATGTG
    AATGACTGGA GACAGCATTC TATTTACAAG AACGGCTACT GCCCAAACCA CCCCGTCATT
    CAGTGGTTCT GGAAGGCTGT GCTACTCATG GACGCCGAAA AGCGTATCCG GTTACTGCAG
    TTTGTCACAG GGACATCGCG AGTACCTATG AATGGATTTG CCGAACTTTA TGGTTCCAAT
    GGTCCTCAGC TGTTTACAAT AGAGCAATGG GGCAGTCCTG AGAAACTGCC CAGAGCTCAC
    ACATGCTTTA ATCGCCTTGA CTTACCTCCA TATGAAACCT TTGAAGATTT ACGAGAGAAA
    CTTCTCATGG CCGTGGAAAA TGCTCAAGGA TTTGAAGGGG TGGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001138439.1
    CDS436..3363
    Translation

    Target ORF information:

    RefSeq Version NM_001144967.3
    Organism Homo sapiens(human)
    Definition Homo sapiens NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant j, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001144967.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGCGACCG GGCTCGGGGA GCCGGTCTAT GGACTTTCCG AAGACGAGGG AGAGTCCCGT 
    ATTCTCAGAG TAAAAGTTGT TTCTGGAATT GATCTCGCCA AAAAGGACAT CTTTGGAGCC
    AGTGATCCGT ATGTGAAACT TTCATTGTAC GTAGCGGATG AGAATAGAGA ACTTGCTTTG
    GTCCAGACAA AAACAATTAA AAAGACACTG AACCCAAAAT GGAATGAAGA ATTTTATTTC
    AGGGTAAACC CATCTAATCA CAGACTCCTA TTTGAAGTAT TTGACGAAAA TAGACTGACA
    CGAGACGACT TCCTGGGCCA GGTGGACGTG CCCCTTAGTC ACCTTCCGAC AGAAGATCCA
    ACCATGGAGC GACCCTATAC ATTTAAGGAC TTTCTCCTCA GACCAAGAAG TCATAAGTCT
    CGAGTTAAGG GATTTTTGCG ATTGAAAATG GCCTATATGC CAAAAAATGG AGGTCAAGAT
    GAAGAAAACA GTGACCAGAG GGATGACATG GAGCATGGAT GGGAAGTTGT TGACTCAAAT
    GACTCGGCTT CTCAGCACCA AGAGGAACTT CCTCCTCCTC CTCTGCCTCC CGGGTGGGAA
    GAAAAAGTGG ACAATTTAGG CCGAACTTAC TATGTCAACC ACAACAACCG GACCACTCAG
    TGGCACAGAC CAAGCCTGAT GGACGTGTCC TCGGAGTCGG ACAATAACAT CAGACAGATC
    AACCAGGAGG CAGCACACCG GCGCTTCCGC TCCCGCAGGC ACATCAGCGA AGACTTGGAG
    CCCGAGCCCT CGGAGGGCGG GGATGTCCCC GAGCCTTGGG AGACCATTTC AGAGGAAGTG
    AATATCGCTG GAGACTCTCT CGGTCTGGCT CTGCCCCCAC CACCGGCCTC CCCAGGATCT
    CGGACCAGCC CTCAGGAGCT GTCAGAGGAA CTAAGCAGAA GGCTTCAGAT CACTCCAGAC
    TCCAATGGGG AACAGTTCAG CTCTTTGATT CAAAGAGAAC CCTCCTCAAG GTTGAGGTCA
    TGCAGTGTCA CCGACGCAGT TGCAGAACAG GGCCATCTAC CACCGCCCAG TGCCCCAGCT
    GGGAGAGCGC GTTCATCAAC TGTCACGGGT GGTGAGGAAC CAACGCCATC AGTGGCCTAT
    GTACATACCA CGCCGGGTCT GCCTTCAGGC TGGGAAGAAA GAAAAGATGC TAAGGGGCGC
    ACATACTATG TCAATCATAA CAATCGAACC ACAACTTGGA CTCGACCTAT CATGCAGCTT
    GCAGAAGATG GTGCGTCCGG ATCAGCCACA AACAGTAACA ACCATCTAAT CGAGCCTCAG
    ATCCGCCGGC CTCGTAGCCT CAGCTCGCCA ACAGTAACTT TATCTGCCCC GCTGGAGGGT
    GCCAAGGACT CACCCGTACG TCGGGCTGTG AAAGACACCC TTTCCAACCC ACAGTCCCCA
    CAGCCATCAC CTTACAACTC CCCCAAACCA CAACACAAAG TCACACAGAG CTTCTTGCCA
    CCCGGCTGGG AAATGAGGAT AGCGCCAAAC GGCCGGCCCT TCTTCATTGA TCATAACACA
    AAGACTACAA CCTGGGAAGA TCCACGTTTG AAATTTCCAG TACATATGCG GTCAAAGACA
    TCTTTAAACC CCAATGACCT TGGCCCCCTT CCTCCTGGCT GGGAAGAAAG AATTCACTTG
    GATGGCCGAA CGTTTTATAT TGATCATAAT AGCAAAATTA CTCAGTGGGA AGACCCAAGA
    CTGCAGAACC CAGCTATTAC TGGTCCGGCT GTCCCTTACT CCAGAGAATT TAAGCAGAAA
    TATGACTACT TCAGGAAGAA ATTAAAGAAA CCTGCTGATA TCCCCAATAG GTTTGAAATG
    AAACTTCACA GAAATAACAT ATTTGAAGAG TCCTATCGGA GAATTATGTC CGTGAAAAGA
    CCAGATGTCC TAAAAGCTAG ACTGTGGATT GAGTTTGAAT CAGAGAAAGG TCTTGACTAT
    GGGGGTGTGG CCAGAGAATG GTTCTTCTTA CTGTCCAAAG AGATGTTCAA CCCCTACTAC
    GGCCTCTTTG AGTACTCTGC CACGGACAAC TACACCCTTC AGATCAACCC TAATTCAGGC
    CTCTGTAATG AGGATCATTT GTCCTACTTC ACTTTTATTG GAAGAGTTGC TGGTCTGGCC
    GTATTTCATG GGAAGCTCTT AGATGGTTTC TTCATTAGAC CATTTTACAA GATGATGTTG
    GGAAAGCAGA TAACCCTGAA TGACATGGAA TCTGTGGATA GTGAATATTA CAACTCTTTG
    AAATGGATCC TGGAGAATGA CCCTACTGAG CTGGACCTCA TGTTCTGCAT AGACGAAGAA
    AACTTTGGAC AGACATATCA AGTGGATTTG AAGCCCAATG GGTCAGAAAT AATGGTCACA
    AATGAAAACA AAAGGGAATA TATCGACTTA GTCATCCAGT GGAGATTTGT GAACAGGGTC
    CAGAAGCAGA TGAACGCCTT CTTGGAGGGA TTCACAGAAC TACTTCCTAT TGATTTGATT
    AAAATTTTTG ATGAAAATGA GCTGGAGTTG CTCATGTGCG GCCTCGGTGA TGTGGATGTG
    AATGACTGGA GACAGCATTC TATTTACAAG AACGGCTACT GCCCAAACCA CCCCGTCATT
    CAGTGGTTCT GGAAGGCTGT GCTACTCATG GACGCCGAAA AGCGTATCCG GTTACTGCAG
    TTTGTCACAG GGACATCGCG AGTACCTATG AATGGATTTG CCGAACTTTA TGGTTCCAAT
    GGTCCTCAGC TGTTTACAAT AGAGCAATGG GGCAGTCCTG AGAAACTGCC CAGAGCTCAC
    ACATGCTTTA ATCGCCTTGA CTTACCTCCA TATGAAACCT TTGAAGATTT ACGAGAGAAA
    CTTCTCATGG CCGTGGAAAA TGCTCAAGGA TTTGAAGGGG TGGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu14306
    Clone ID Related Accession (Same CDS sequence) NM_001144965.1 , NM_001144966.2 , XM_017025679.2 , NM_001144966.3 , NM_001144964.1 , XM_017025679.1 , XM_024451129.1 , NM_001144965.2
    Accession Version XM_017025679.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2565bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017025679.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAGCGAC CCTATACATT TAAGGACTTT CTCCTCAGAC CAAGAAGTCA TAAGTCTCGA 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCAA AAAATGGAGG TCAAGATGAA
    GAAAACAGTG ACCAGAGGGA TGACATGGAG CATGGATGGG AAGTTGTTGA CTCAAATGAC
    TCGGCTTCTC AGCACCAAGA GGAACTTCCT CCTCCTCCTC TGCCTCCCGG GTGGGAAGAA
    AAAGTGGACA ATTTAGGCCG AACTTACTAT GTCAACCACA ACAACCGGAC CACTCAGTGG
    CACAGACCAA GCCTGATGGA CGTGTCCTCG GAGTCGGACA ATAACATCAG ACAGATCAAC
    CAGGAGGCAG CACACCGGCG CTTCCGCTCC CGCAGGCACA TCAGCGAAGA CTTGGAGCCC
    GAGCCCTCGG AGGGCGGGGA TGTCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAT
    ATCGCTGGAG ACTCTCTCGG TCTGGCTCTG CCCCCACCAC CGGCCTCCCC AGGATCTCGG
    ACCAGCCCTC AGGAGCTGTC AGAGGAACTA AGCAGAAGGC TTCAGATCAC TCCAGACTCC
    AATGGGGAAC AGTTCAGCTC TTTGATTCAA AGAGAACCCT CCTCAAGGTT GAGGTCATGC
    AGTGTCACCG ACGCAGTTGC AGAACAGGGC CATCTACCAC CGCCCAGTGC CCCAGCTGGG
    AGAGCGCGTT CATCAACTGT CACGGGTGGT GAGGAACCAA CGCCATCAGT GGCCTATGTA
    CATACCACGC CGGGTCTGCC TTCAGGCTGG GAAGAAAGAA AAGATGCTAA GGGGCGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GACCTATCAT GCAGCTTGCA
    GAAGATGGTG CGTCCGGATC AGCCACAAAC AGTAACAACC ATCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CTGCCCCGCT GGAGGGTGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GACACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CTTGCCACCC
    GGCTGGGAAA TGAGGATAGC GCCAAACGGC CGGCCCTTCT TCATTGATCA TAACACAAAG
    ACTACAACCT GGGAAGATCC ACGTTTGAAA TTTCCAGTAC ATATGCGGTC AAAGACATCT
    TTAAACCCCA ATGACCTTGG CCCCCTTCCT CCTGGCTGGG AAGAAAGAAT TCACTTGGAT
    GGCCGAACGT TTTATATTGA TCATAATAGC AAAATTACTC AGTGGGAAGA CCCAAGACTG
    CAGAACCCAG CTATTACTGG TCCGGCTGTC CCTTACTCCA GAGAATTTAA GCAGAAATAT
    GACTACTTCA GGAAGAAATT AAAGAAACCT GCTGATATCC CCAATAGGTT TGAAATGAAA
    CTTCACAGAA ATAACATATT TGAAGAGTCC TATCGGAGAA TTATGTCCGT GAAAAGACCA
    GATGTCCTAA AAGCTAGACT GTGGATTGAG TTTGAATCAG AGAAAGGTCT TGACTATGGG
    GGTGTGGCCA GAGAATGGTT CTTCTTACTG TCCAAAGAGA TGTTCAACCC CTACTACGGC
    CTCTTTGAGT ACTCTGCCAC GGACAACTAC ACCCTTCAGA TCAACCCTAA TTCAGGCCTC
    TGTAATGAGG ATCATTTGTC CTACTTCACT TTTATTGGAA GAGTTGCTGG TCTGGCCGTA
    TTTCATGGGA AGCTCTTAGA TGGTTTCTTC ATTAGACCAT TTTACAAGAT GATGTTGGGA
    AAGCAGATAA CCCTGAATGA CATGGAATCT GTGGATAGTG AATATTACAA CTCTTTGAAA
    TGGATCCTGG AGAATGACCC TACTGAGCTG GACCTCATGT TCTGCATAGA CGAAGAAAAC
    TTTGGACAGA CATATCAAGT GGATTTGAAG CCCAATGGGT CAGAAATAAT GGTCACAAAT
    GAAAACAAAA GGGAATATAT CGACTTAGTC ATCCAGTGGA GATTTGTGAA CAGGGTCCAG
    AAGCAGATGA ACGCCTTCTT GGAGGGATTC ACAGAACTAC TTCCTATTGA TTTGATTAAA
    ATTTTTGATG AAAATGAGCT GGAGTTGCTC ATGTGCGGCC TCGGTGATGT GGATGTGAAT
    GACTGGAGAC AGCATTCTAT TTACAAGAAC GGCTACTGCC CAAACCACCC CGTCATTCAG
    TGGTTCTGGA AGGCTGTGCT ACTCATGGAC GCCGAAAAGC GTATCCGGTT ACTGCAGTTT
    GTCACAGGGA CATCGCGAGT ACCTATGAAT GGATTTGCCG AACTTTATGG TTCCAATGGT
    CCTCAGCTGT TTACAATAGA GCAATGGGGC AGTCCTGAGA AACTGCCCAG AGCTCACACA
    TGCTTTAATC GCCTTGACTT ACCTCCATAT GAAACCTTTG AAGATTTACG AGAGAAACTT
    CTCATGGCCG TGGAAAATGC TCAAGGATTT GAAGGGGTGG ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016881168.1
    CDS586..3150
    Translation

    Target ORF information:

    RefSeq Version XM_017025679.2
    Organism Homo sapiens(human)
    Definition Homo sapiens NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant X20, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017025679.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAGCGAC CCTATACATT TAAGGACTTT CTCCTCAGAC CAAGAAGTCA TAAGTCTCGA 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCAA AAAATGGAGG TCAAGATGAA
    GAAAACAGTG ACCAGAGGGA TGACATGGAG CATGGATGGG AAGTTGTTGA CTCAAATGAC
    TCGGCTTCTC AGCACCAAGA GGAACTTCCT CCTCCTCCTC TGCCTCCCGG GTGGGAAGAA
    AAAGTGGACA ATTTAGGCCG AACTTACTAT GTCAACCACA ACAACCGGAC CACTCAGTGG
    CACAGACCAA GCCTGATGGA CGTGTCCTCG GAGTCGGACA ATAACATCAG ACAGATCAAC
    CAGGAGGCAG CACACCGGCG CTTCCGCTCC CGCAGGCACA TCAGCGAAGA CTTGGAGCCC
    GAGCCCTCGG AGGGCGGGGA TGTCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAT
    ATCGCTGGAG ACTCTCTCGG TCTGGCTCTG CCCCCACCAC CGGCCTCCCC AGGATCTCGG
    ACCAGCCCTC AGGAGCTGTC AGAGGAACTA AGCAGAAGGC TTCAGATCAC TCCAGACTCC
    AATGGGGAAC AGTTCAGCTC TTTGATTCAA AGAGAACCCT CCTCAAGGTT GAGGTCATGC
    AGTGTCACCG ACGCAGTTGC AGAACAGGGC CATCTACCAC CGCCCAGTGC CCCAGCTGGG
    AGAGCGCGTT CATCAACTGT CACGGGTGGT GAGGAACCAA CGCCATCAGT GGCCTATGTA
    CATACCACGC CGGGTCTGCC TTCAGGCTGG GAAGAAAGAA AAGATGCTAA GGGGCGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GACCTATCAT GCAGCTTGCA
    GAAGATGGTG CGTCCGGATC AGCCACAAAC AGTAACAACC ATCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CTGCCCCGCT GGAGGGTGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GACACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CTTGCCACCC
    GGCTGGGAAA TGAGGATAGC GCCAAACGGC CGGCCCTTCT TCATTGATCA TAACACAAAG
    ACTACAACCT GGGAAGATCC ACGTTTGAAA TTTCCAGTAC ATATGCGGTC AAAGACATCT
    TTAAACCCCA ATGACCTTGG CCCCCTTCCT CCTGGCTGGG AAGAAAGAAT TCACTTGGAT
    GGCCGAACGT TTTATATTGA TCATAATAGC AAAATTACTC AGTGGGAAGA CCCAAGACTG
    CAGAACCCAG CTATTACTGG TCCGGCTGTC CCTTACTCCA GAGAATTTAA GCAGAAATAT
    GACTACTTCA GGAAGAAATT AAAGAAACCT GCTGATATCC CCAATAGGTT TGAAATGAAA
    CTTCACAGAA ATAACATATT TGAAGAGTCC TATCGGAGAA TTATGTCCGT GAAAAGACCA
    GATGTCCTAA AAGCTAGACT GTGGATTGAG TTTGAATCAG AGAAAGGTCT TGACTATGGG
    GGTGTGGCCA GAGAATGGTT CTTCTTACTG TCCAAAGAGA TGTTCAACCC CTACTACGGC
    CTCTTTGAGT ACTCTGCCAC GGACAACTAC ACCCTTCAGA TCAACCCTAA TTCAGGCCTC
    TGTAATGAGG ATCATTTGTC CTACTTCACT TTTATTGGAA GAGTTGCTGG TCTGGCCGTA
    TTTCATGGGA AGCTCTTAGA TGGTTTCTTC ATTAGACCAT TTTACAAGAT GATGTTGGGA
    AAGCAGATAA CCCTGAATGA CATGGAATCT GTGGATAGTG AATATTACAA CTCTTTGAAA
    TGGATCCTGG AGAATGACCC TACTGAGCTG GACCTCATGT TCTGCATAGA CGAAGAAAAC
    TTTGGACAGA CATATCAAGT GGATTTGAAG CCCAATGGGT CAGAAATAAT GGTCACAAAT
    GAAAACAAAA GGGAATATAT CGACTTAGTC ATCCAGTGGA GATTTGTGAA CAGGGTCCAG
    AAGCAGATGA ACGCCTTCTT GGAGGGATTC ACAGAACTAC TTCCTATTGA TTTGATTAAA
    ATTTTTGATG AAAATGAGCT GGAGTTGCTC ATGTGCGGCC TCGGTGATGT GGATGTGAAT
    GACTGGAGAC AGCATTCTAT TTACAAGAAC GGCTACTGCC CAAACCACCC CGTCATTCAG
    TGGTTCTGGA AGGCTGTGCT ACTCATGGAC GCCGAAAAGC GTATCCGGTT ACTGCAGTTT
    GTCACAGGGA CATCGCGAGT ACCTATGAAT GGATTTGCCG AACTTTATGG TTCCAATGGT
    CCTCAGCTGT TTACAATAGA GCAATGGGGC AGTCCTGAGA AACTGCCCAG AGCTCACACA
    TGCTTTAATC GCCTTGACTT ACCTCCATAT GAAACCTTTG AAGATTTACG AGAGAAACTT
    CTCATGGCCG TGGAAAATGC TCAAGGATTT GAAGGGGTGG ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu14306
    Clone ID Related Accession (Same CDS sequence) NM_001144965.1 , NM_001144966.2 , XM_017025679.2 , NM_001144966.3 , NM_001144964.1 , XM_017025679.1 , XM_024451129.1 , NM_001144965.2
    Accession Version XM_017025679.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2565bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAGCGAC CCTATACATT TAAGGACTTT CTCCTCAGAC CAAGAAGTCA TAAGTCTCGA 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCAA AAAATGGAGG TCAAGATGAA
    GAAAACAGTG ACCAGAGGGA TGACATGGAG CATGGATGGG AAGTTGTTGA CTCAAATGAC
    TCGGCTTCTC AGCACCAAGA GGAACTTCCT CCTCCTCCTC TGCCTCCCGG GTGGGAAGAA
    AAAGTGGACA ATTTAGGCCG AACTTACTAT GTCAACCACA ACAACCGGAC CACTCAGTGG
    CACAGACCAA GCCTGATGGA CGTGTCCTCG GAGTCGGACA ATAACATCAG ACAGATCAAC
    CAGGAGGCAG CACACCGGCG CTTCCGCTCC CGCAGGCACA TCAGCGAAGA CTTGGAGCCC
    GAGCCCTCGG AGGGCGGGGA TGTCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAT
    ATCGCTGGAG ACTCTCTCGG TCTGGCTCTG CCCCCACCAC CGGCCTCCCC AGGATCTCGG
    ACCAGCCCTC AGGAGCTGTC AGAGGAACTA AGCAGAAGGC TTCAGATCAC TCCAGACTCC
    AATGGGGAAC AGTTCAGCTC TTTGATTCAA AGAGAACCCT CCTCAAGGTT GAGGTCATGC
    AGTGTCACCG ACGCAGTTGC AGAACAGGGC CATCTACCAC CGCCCAGTGC CCCAGCTGGG
    AGAGCGCGTT CATCAACTGT CACGGGTGGT GAGGAACCAA CGCCATCAGT GGCCTATGTA
    CATACCACGC CGGGTCTGCC TTCAGGCTGG GAAGAAAGAA AAGATGCTAA GGGGCGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GACCTATCAT GCAGCTTGCA
    GAAGATGGTG CGTCCGGATC AGCCACAAAC AGTAACAACC ATCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CTGCCCCGCT GGAGGGTGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GACACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CTTGCCACCC
    GGCTGGGAAA TGAGGATAGC GCCAAACGGC CGGCCCTTCT TCATTGATCA TAACACAAAG
    ACTACAACCT GGGAAGATCC ACGTTTGAAA TTTCCAGTAC ATATGCGGTC AAAGACATCT
    TTAAACCCCA ATGACCTTGG CCCCCTTCCT CCTGGCTGGG AAGAAAGAAT TCACTTGGAT
    GGCCGAACGT TTTATATTGA TCATAATAGC AAAATTACTC AGTGGGAAGA CCCAAGACTG
    CAGAACCCAG CTATTACTGG TCCGGCTGTC CCTTACTCCA GAGAATTTAA GCAGAAATAT
    GACTACTTCA GGAAGAAATT AAAGAAACCT GCTGATATCC CCAATAGGTT TGAAATGAAA
    CTTCACAGAA ATAACATATT TGAAGAGTCC TATCGGAGAA TTATGTCCGT GAAAAGACCA
    GATGTCCTAA AAGCTAGACT GTGGATTGAG TTTGAATCAG AGAAAGGTCT TGACTATGGG
    GGTGTGGCCA GAGAATGGTT CTTCTTACTG TCCAAAGAGA TGTTCAACCC CTACTACGGC
    CTCTTTGAGT ACTCTGCCAC GGACAACTAC ACCCTTCAGA TCAACCCTAA TTCAGGCCTC
    TGTAATGAGG ATCATTTGTC CTACTTCACT TTTATTGGAA GAGTTGCTGG TCTGGCCGTA
    TTTCATGGGA AGCTCTTAGA TGGTTTCTTC ATTAGACCAT TTTACAAGAT GATGTTGGGA
    AAGCAGATAA CCCTGAATGA CATGGAATCT GTGGATAGTG AATATTACAA CTCTTTGAAA
    TGGATCCTGG AGAATGACCC TACTGAGCTG GACCTCATGT TCTGCATAGA CGAAGAAAAC
    TTTGGACAGA CATATCAAGT GGATTTGAAG CCCAATGGGT CAGAAATAAT GGTCACAAAT
    GAAAACAAAA GGGAATATAT CGACTTAGTC ATCCAGTGGA GATTTGTGAA CAGGGTCCAG
    AAGCAGATGA ACGCCTTCTT GGAGGGATTC ACAGAACTAC TTCCTATTGA TTTGATTAAA
    ATTTTTGATG AAAATGAGCT GGAGTTGCTC ATGTGCGGCC TCGGTGATGT GGATGTGAAT
    GACTGGAGAC AGCATTCTAT TTACAAGAAC GGCTACTGCC CAAACCACCC CGTCATTCAG
    TGGTTCTGGA AGGCTGTGCT ACTCATGGAC GCCGAAAAGC GTATCCGGTT ACTGCAGTTT
    GTCACAGGGA CATCGCGAGT ACCTATGAAT GGATTTGCCG AACTTTATGG TTCCAATGGT
    CCTCAGCTGT TTACAATAGA GCAATGGGGC AGTCCTGAGA AACTGCCCAG AGCTCACACA
    TGCTTTAATC GCCTTGACTT ACCTCCATAT GAAACCTTTG AAGATTTACG AGAGAAACTT
    CTCATGGCCG TGGAAAATGC TCAAGGATTT GAAGGGGTGG ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016881168.1
    CDS571..3135
    Translation

    Target ORF information:

    RefSeq Version XM_017025679.1
    Organism Homo sapiens(human)
    Definition Homo sapiens neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017025679.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAGCGAC CCTATACATT TAAGGACTTT CTCCTCAGAC CAAGAAGTCA TAAGTCTCGA 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCAA AAAATGGAGG TCAAGATGAA
    GAAAACAGTG ACCAGAGGGA TGACATGGAG CATGGATGGG AAGTTGTTGA CTCAAATGAC
    TCGGCTTCTC AGCACCAAGA GGAACTTCCT CCTCCTCCTC TGCCTCCCGG GTGGGAAGAA
    AAAGTGGACA ATTTAGGCCG AACTTACTAT GTCAACCACA ACAACCGGAC CACTCAGTGG
    CACAGACCAA GCCTGATGGA CGTGTCCTCG GAGTCGGACA ATAACATCAG ACAGATCAAC
    CAGGAGGCAG CACACCGGCG CTTCCGCTCC CGCAGGCACA TCAGCGAAGA CTTGGAGCCC
    GAGCCCTCGG AGGGCGGGGA TGTCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAT
    ATCGCTGGAG ACTCTCTCGG TCTGGCTCTG CCCCCACCAC CGGCCTCCCC AGGATCTCGG
    ACCAGCCCTC AGGAGCTGTC AGAGGAACTA AGCAGAAGGC TTCAGATCAC TCCAGACTCC
    AATGGGGAAC AGTTCAGCTC TTTGATTCAA AGAGAACCCT CCTCAAGGTT GAGGTCATGC
    AGTGTCACCG ACGCAGTTGC AGAACAGGGC CATCTACCAC CGCCCAGTGC CCCAGCTGGG
    AGAGCGCGTT CATCAACTGT CACGGGTGGT GAGGAACCAA CGCCATCAGT GGCCTATGTA
    CATACCACGC CGGGTCTGCC TTCAGGCTGG GAAGAAAGAA AAGATGCTAA GGGGCGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GACCTATCAT GCAGCTTGCA
    GAAGATGGTG CGTCCGGATC AGCCACAAAC AGTAACAACC ATCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CTGCCCCGCT GGAGGGTGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GACACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CTTGCCACCC
    GGCTGGGAAA TGAGGATAGC GCCAAACGGC CGGCCCTTCT TCATTGATCA TAACACAAAG
    ACTACAACCT GGGAAGATCC ACGTTTGAAA TTTCCAGTAC ATATGCGGTC AAAGACATCT
    TTAAACCCCA ATGACCTTGG CCCCCTTCCT CCTGGCTGGG AAGAAAGAAT TCACTTGGAT
    GGCCGAACGT TTTATATTGA TCATAATAGC AAAATTACTC AGTGGGAAGA CCCAAGACTG
    CAGAACCCAG CTATTACTGG TCCGGCTGTC CCTTACTCCA GAGAATTTAA GCAGAAATAT
    GACTACTTCA GGAAGAAATT AAAGAAACCT GCTGATATCC CCAATAGGTT TGAAATGAAA
    CTTCACAGAA ATAACATATT TGAAGAGTCC TATCGGAGAA TTATGTCCGT GAAAAGACCA
    GATGTCCTAA AAGCTAGACT GTGGATTGAG TTTGAATCAG AGAAAGGTCT TGACTATGGG
    GGTGTGGCCA GAGAATGGTT CTTCTTACTG TCCAAAGAGA TGTTCAACCC CTACTACGGC
    CTCTTTGAGT ACTCTGCCAC GGACAACTAC ACCCTTCAGA TCAACCCTAA TTCAGGCCTC
    TGTAATGAGG ATCATTTGTC CTACTTCACT TTTATTGGAA GAGTTGCTGG TCTGGCCGTA
    TTTCATGGGA AGCTCTTAGA TGGTTTCTTC ATTAGACCAT TTTACAAGAT GATGTTGGGA
    AAGCAGATAA CCCTGAATGA CATGGAATCT GTGGATAGTG AATATTACAA CTCTTTGAAA
    TGGATCCTGG AGAATGACCC TACTGAGCTG GACCTCATGT TCTGCATAGA CGAAGAAAAC
    TTTGGACAGA CATATCAAGT GGATTTGAAG CCCAATGGGT CAGAAATAAT GGTCACAAAT
    GAAAACAAAA GGGAATATAT CGACTTAGTC ATCCAGTGGA GATTTGTGAA CAGGGTCCAG
    AAGCAGATGA ACGCCTTCTT GGAGGGATTC ACAGAACTAC TTCCTATTGA TTTGATTAAA
    ATTTTTGATG AAAATGAGCT GGAGTTGCTC ATGTGCGGCC TCGGTGATGT GGATGTGAAT
    GACTGGAGAC AGCATTCTAT TTACAAGAAC GGCTACTGCC CAAACCACCC CGTCATTCAG
    TGGTTCTGGA AGGCTGTGCT ACTCATGGAC GCCGAAAAGC GTATCCGGTT ACTGCAGTTT
    GTCACAGGGA CATCGCGAGT ACCTATGAAT GGATTTGCCG AACTTTATGG TTCCAATGGT
    CCTCAGCTGT TTACAATAGA GCAATGGGGC AGTCCTGAGA AACTGCCCAG AGCTCACACA
    TGCTTTAATC GCCTTGACTT ACCTCCATAT GAAACCTTTG AAGATTTACG AGAGAAACTT
    CTCATGGCCG TGGAAAATGC TCAAGGATTT GAAGGGGTGG ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu14306
    Clone ID Related Accession (Same CDS sequence) NM_001144965.1 , NM_001144966.2 , XM_017025679.2 , NM_001144966.3 , NM_001144964.1 , XM_017025679.1 , XM_024451129.1 , NM_001144965.2
    Accession Version XM_024451129.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2565bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAGCGAC CCTATACATT TAAGGACTTT CTCCTCAGAC CAAGAAGTCA TAAGTCTCGA 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCAA AAAATGGAGG TCAAGATGAA
    GAAAACAGTG ACCAGAGGGA TGACATGGAG CATGGATGGG AAGTTGTTGA CTCAAATGAC
    TCGGCTTCTC AGCACCAAGA GGAACTTCCT CCTCCTCCTC TGCCTCCCGG GTGGGAAGAA
    AAAGTGGACA ATTTAGGCCG AACTTACTAT GTCAACCACA ACAACCGGAC CACTCAGTGG
    CACAGACCAA GCCTGATGGA CGTGTCCTCG GAGTCGGACA ATAACATCAG ACAGATCAAC
    CAGGAGGCAG CACACCGGCG CTTCCGCTCC CGCAGGCACA TCAGCGAAGA CTTGGAGCCC
    GAGCCCTCGG AGGGCGGGGA TGTCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAT
    ATCGCTGGAG ACTCTCTCGG TCTGGCTCTG CCCCCACCAC CGGCCTCCCC AGGATCTCGG
    ACCAGCCCTC AGGAGCTGTC AGAGGAACTA AGCAGAAGGC TTCAGATCAC TCCAGACTCC
    AATGGGGAAC AGTTCAGCTC TTTGATTCAA AGAGAACCCT CCTCAAGGTT GAGGTCATGC
    AGTGTCACCG ACGCAGTTGC AGAACAGGGC CATCTACCAC CGCCCAGTGC CCCAGCTGGG
    AGAGCGCGTT CATCAACTGT CACGGGTGGT GAGGAACCAA CGCCATCAGT GGCCTATGTA
    CATACCACGC CGGGTCTGCC TTCAGGCTGG GAAGAAAGAA AAGATGCTAA GGGGCGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GACCTATCAT GCAGCTTGCA
    GAAGATGGTG CGTCCGGATC AGCCACAAAC AGTAACAACC ATCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CTGCCCCGCT GGAGGGTGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GACACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CTTGCCACCC
    GGCTGGGAAA TGAGGATAGC GCCAAACGGC CGGCCCTTCT TCATTGATCA TAACACAAAG
    ACTACAACCT GGGAAGATCC ACGTTTGAAA TTTCCAGTAC ATATGCGGTC AAAGACATCT
    TTAAACCCCA ATGACCTTGG CCCCCTTCCT CCTGGCTGGG AAGAAAGAAT TCACTTGGAT
    GGCCGAACGT TTTATATTGA TCATAATAGC AAAATTACTC AGTGGGAAGA CCCAAGACTG
    CAGAACCCAG CTATTACTGG TCCGGCTGTC CCTTACTCCA GAGAATTTAA GCAGAAATAT
    GACTACTTCA GGAAGAAATT AAAGAAACCT GCTGATATCC CCAATAGGTT TGAAATGAAA
    CTTCACAGAA ATAACATATT TGAAGAGTCC TATCGGAGAA TTATGTCCGT GAAAAGACCA
    GATGTCCTAA AAGCTAGACT GTGGATTGAG TTTGAATCAG AGAAAGGTCT TGACTATGGG
    GGTGTGGCCA GAGAATGGTT CTTCTTACTG TCCAAAGAGA TGTTCAACCC CTACTACGGC
    CTCTTTGAGT ACTCTGCCAC GGACAACTAC ACCCTTCAGA TCAACCCTAA TTCAGGCCTC
    TGTAATGAGG ATCATTTGTC CTACTTCACT TTTATTGGAA GAGTTGCTGG TCTGGCCGTA
    TTTCATGGGA AGCTCTTAGA TGGTTTCTTC ATTAGACCAT TTTACAAGAT GATGTTGGGA
    AAGCAGATAA CCCTGAATGA CATGGAATCT GTGGATAGTG AATATTACAA CTCTTTGAAA
    TGGATCCTGG AGAATGACCC TACTGAGCTG GACCTCATGT TCTGCATAGA CGAAGAAAAC
    TTTGGACAGA CATATCAAGT GGATTTGAAG CCCAATGGGT CAGAAATAAT GGTCACAAAT
    GAAAACAAAA GGGAATATAT CGACTTAGTC ATCCAGTGGA GATTTGTGAA CAGGGTCCAG
    AAGCAGATGA ACGCCTTCTT GGAGGGATTC ACAGAACTAC TTCCTATTGA TTTGATTAAA
    ATTTTTGATG AAAATGAGCT GGAGTTGCTC ATGTGCGGCC TCGGTGATGT GGATGTGAAT
    GACTGGAGAC AGCATTCTAT TTACAAGAAC GGCTACTGCC CAAACCACCC CGTCATTCAG
    TGGTTCTGGA AGGCTGTGCT ACTCATGGAC GCCGAAAAGC GTATCCGGTT ACTGCAGTTT
    GTCACAGGGA CATCGCGAGT ACCTATGAAT GGATTTGCCG AACTTTATGG TTCCAATGGT
    CCTCAGCTGT TTACAATAGA GCAATGGGGC AGTCCTGAGA AACTGCCCAG AGCTCACACA
    TGCTTTAATC GCCTTGACTT ACCTCCATAT GAAACCTTTG AAGATTTACG AGAGAAACTT
    CTCATGGCCG TGGAAAATGC TCAAGGATTT GAAGGGGTGG ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024306897.1
    CDS568..3132
    Translation

    Target ORF information:

    RefSeq Version XM_024451129.1
    Organism Homo sapiens(human)
    Definition Homo sapiens NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024451129.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAGCGAC CCTATACATT TAAGGACTTT CTCCTCAGAC CAAGAAGTCA TAAGTCTCGA 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCAA AAAATGGAGG TCAAGATGAA
    GAAAACAGTG ACCAGAGGGA TGACATGGAG CATGGATGGG AAGTTGTTGA CTCAAATGAC
    TCGGCTTCTC AGCACCAAGA GGAACTTCCT CCTCCTCCTC TGCCTCCCGG GTGGGAAGAA
    AAAGTGGACA ATTTAGGCCG AACTTACTAT GTCAACCACA ACAACCGGAC CACTCAGTGG
    CACAGACCAA GCCTGATGGA CGTGTCCTCG GAGTCGGACA ATAACATCAG ACAGATCAAC
    CAGGAGGCAG CACACCGGCG CTTCCGCTCC CGCAGGCACA TCAGCGAAGA CTTGGAGCCC
    GAGCCCTCGG AGGGCGGGGA TGTCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAT
    ATCGCTGGAG ACTCTCTCGG TCTGGCTCTG CCCCCACCAC CGGCCTCCCC AGGATCTCGG
    ACCAGCCCTC AGGAGCTGTC AGAGGAACTA AGCAGAAGGC TTCAGATCAC TCCAGACTCC
    AATGGGGAAC AGTTCAGCTC TTTGATTCAA AGAGAACCCT CCTCAAGGTT GAGGTCATGC
    AGTGTCACCG ACGCAGTTGC AGAACAGGGC CATCTACCAC CGCCCAGTGC CCCAGCTGGG
    AGAGCGCGTT CATCAACTGT CACGGGTGGT GAGGAACCAA CGCCATCAGT GGCCTATGTA
    CATACCACGC CGGGTCTGCC TTCAGGCTGG GAAGAAAGAA AAGATGCTAA GGGGCGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GACCTATCAT GCAGCTTGCA
    GAAGATGGTG CGTCCGGATC AGCCACAAAC AGTAACAACC ATCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CTGCCCCGCT GGAGGGTGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GACACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CTTGCCACCC
    GGCTGGGAAA TGAGGATAGC GCCAAACGGC CGGCCCTTCT TCATTGATCA TAACACAAAG
    ACTACAACCT GGGAAGATCC ACGTTTGAAA TTTCCAGTAC ATATGCGGTC AAAGACATCT
    TTAAACCCCA ATGACCTTGG CCCCCTTCCT CCTGGCTGGG AAGAAAGAAT TCACTTGGAT
    GGCCGAACGT TTTATATTGA TCATAATAGC AAAATTACTC AGTGGGAAGA CCCAAGACTG
    CAGAACCCAG CTATTACTGG TCCGGCTGTC CCTTACTCCA GAGAATTTAA GCAGAAATAT
    GACTACTTCA GGAAGAAATT AAAGAAACCT GCTGATATCC CCAATAGGTT TGAAATGAAA
    CTTCACAGAA ATAACATATT TGAAGAGTCC TATCGGAGAA TTATGTCCGT GAAAAGACCA
    GATGTCCTAA AAGCTAGACT GTGGATTGAG TTTGAATCAG AGAAAGGTCT TGACTATGGG
    GGTGTGGCCA GAGAATGGTT CTTCTTACTG TCCAAAGAGA TGTTCAACCC CTACTACGGC
    CTCTTTGAGT ACTCTGCCAC GGACAACTAC ACCCTTCAGA TCAACCCTAA TTCAGGCCTC
    TGTAATGAGG ATCATTTGTC CTACTTCACT TTTATTGGAA GAGTTGCTGG TCTGGCCGTA
    TTTCATGGGA AGCTCTTAGA TGGTTTCTTC ATTAGACCAT TTTACAAGAT GATGTTGGGA
    AAGCAGATAA CCCTGAATGA CATGGAATCT GTGGATAGTG AATATTACAA CTCTTTGAAA
    TGGATCCTGG AGAATGACCC TACTGAGCTG GACCTCATGT TCTGCATAGA CGAAGAAAAC
    TTTGGACAGA CATATCAAGT GGATTTGAAG CCCAATGGGT CAGAAATAAT GGTCACAAAT
    GAAAACAAAA GGGAATATAT CGACTTAGTC ATCCAGTGGA GATTTGTGAA CAGGGTCCAG
    AAGCAGATGA ACGCCTTCTT GGAGGGATTC ACAGAACTAC TTCCTATTGA TTTGATTAAA
    ATTTTTGATG AAAATGAGCT GGAGTTGCTC ATGTGCGGCC TCGGTGATGT GGATGTGAAT
    GACTGGAGAC AGCATTCTAT TTACAAGAAC GGCTACTGCC CAAACCACCC CGTCATTCAG
    TGGTTCTGGA AGGCTGTGCT ACTCATGGAC GCCGAAAAGC GTATCCGGTT ACTGCAGTTT
    GTCACAGGGA CATCGCGAGT ACCTATGAAT GGATTTGCCG AACTTTATGG TTCCAATGGT
    CCTCAGCTGT TTACAATAGA GCAATGGGGC AGTCCTGAGA AACTGCCCAG AGCTCACACA
    TGCTTTAATC GCCTTGACTT ACCTCCATAT GAAACCTTTG AAGATTTACG AGAGAAACTT
    CTCATGGCCG TGGAAAATGC TCAAGGATTT GAAGGGGTGG ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu14254
    Clone ID Related Accession (Same CDS sequence) XM_024451136.1 , XM_024451137.1 , NM_001144971.2 , NM_001144970.3 , NM_001144971.1 , NM_001144970.2 , XM_024451135.1 , XM_024451131.1
    Accession Version NM_001144971.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2505bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-20
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform 6
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC107896.7, DQ181796.1, BP260852.1, AF070601.1, DA759940.1, AC090236.4 and DB317092.1. On May 31, 2019 this sequence version replaced NM_001144971.1. Transcript Variant: This variant (k) uses two alternate exons in its 5' end and lacks a downstream in-frame exon, compared to variant j. These differences result in the use of a downstream in-frame start codon and a protein (isoform 6) with a shorter N-terminus, compared to isoform 1. Variants c and k encode the same isoform (6). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ181796.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGAGCGAC CCTATACATT TAAGGACTTT CTCCTCAGAC CAAGAAGTCA TAAGTCTCGA 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCAA AAAATGGAGG TCAAGATGAA
    GAAAACAGTG ACCAGAGGGA TGACATGGAG CATGGATGGG AAGTTGTTGA CTCAAATGAC
    TCGGCTTCTC AGCACCAAGA GGAACTTCCT CCTCCTCCTC TGCCTCCCGG GTGGGAAGAA
    AAAGTGGACA ATTTAGGCCG AACTTACTAT GTCAACCACA ACAACCGGAC CACTCAGTGG
    CACAGACCAA GCCTGATGGA CGTGTCCTCG GAGTCGGACA ATAACATCAG ACAGATCAAC
    CAGGAGGCAG CACACCGGCG CTTCCGCTCC CGCAGGCACA TCAGCGAAGA CTTGGAGCCC
    GAGCCCTCGG AGGGCGGGGA TGTCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAT
    ATCGCTGGAG ACTCTCTCGG TCTGGCTCTG CCCCCACCAC CGGCCTCCCC AGGATCTCGG
    ACCAGCCCTC AGGAGCTGTC AGAGGAACTA AGCAGAAGGC TTCAGATCAC TCCAGACTCC
    AATGGGGAAC AGTTCAGCTC TTTGATTCAA AGAGAACCCT CCTCAAGGTT GAGGTCATGC
    AGTGTCACCG ACGCAGTTGC AGAACAGGGC CATCTACCAC CGCCATCAGT GGCCTATGTA
    CATACCACGC CGGGTCTGCC TTCAGGCTGG GAAGAAAGAA AAGATGCTAA GGGGCGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GACCTATCAT GCAGCTTGCA
    GAAGATGGTG CGTCCGGATC AGCCACAAAC AGTAACAACC ATCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CTGCCCCGCT GGAGGGTGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GACACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CTTGCCACCC
    GGCTGGGAAA TGAGGATAGC GCCAAACGGC CGGCCCTTCT TCATTGATCA TAACACAAAG
    ACTACAACCT GGGAAGATCC ACGTTTGAAA TTTCCAGTAC ATATGCGGTC AAAGACATCT
    TTAAACCCCA ATGACCTTGG CCCCCTTCCT CCTGGCTGGG AAGAAAGAAT TCACTTGGAT
    GGCCGAACGT TTTATATTGA TCATAATAGC AAAATTACTC AGTGGGAAGA CCCAAGACTG
    CAGAACCCAG CTATTACTGG TCCGGCTGTC CCTTACTCCA GAGAATTTAA GCAGAAATAT
    GACTACTTCA GGAAGAAATT AAAGAAACCT GCTGATATCC CCAATAGGTT TGAAATGAAA
    CTTCACAGAA ATAACATATT TGAAGAGTCC TATCGGAGAA TTATGTCCGT GAAAAGACCA
    GATGTCCTAA AAGCTAGACT GTGGATTGAG TTTGAATCAG AGAAAGGTCT TGACTATGGG
    GGTGTGGCCA GAGAATGGTT CTTCTTACTG TCCAAAGAGA TGTTCAACCC CTACTACGGC
    CTCTTTGAGT ACTCTGCCAC GGACAACTAC ACCCTTCAGA TCAACCCTAA TTCAGGCCTC
    TGTAATGAGG ATCATTTGTC CTACTTCACT TTTATTGGAA GAGTTGCTGG TCTGGCCGTA
    TTTCATGGGA AGCTCTTAGA TGGTTTCTTC ATTAGACCAT TTTACAAGAT GATGTTGGGA
    AAGCAGATAA CCCTGAATGA CATGGAATCT GTGGATAGTG AATATTACAA CTCTTTGAAA
    TGGATCCTGG AGAATGACCC TACTGAGCTG GACCTCATGT TCTGCATAGA CGAAGAAAAC
    TTTGGACAGA CATATCAAGT GGATTTGAAG CCCAATGGGT CAGAAATAAT GGTCACAAAT
    GAAAACAAAA GGGAATATAT CGACTTAGTC ATCCAGTGGA GATTTGTGAA CAGGGTCCAG
    AAGCAGATGA ACGCCTTCTT GGAGGGATTC ACAGAACTAC TTCCTATTGA TTTGATTAAA
    ATTTTTGATG AAAATGAGCT GGAGTTGCTC ATGTGCGGCC TCGGTGATGT GGATGTGAAT
    GACTGGAGAC AGCATTCTAT TTACAAGAAC GGCTACTGCC CAAACCACCC CGTCATTCAG
    TGGTTCTGGA AGGCTGTGCT ACTCATGGAC GCCGAAAAGC GTATCCGGTT ACTGCAGTTT
    GTCACAGGGA CATCGCGAGT ACCTATGAAT GGATTTGCCG AACTTTATGG TTCCAATGGT
    CCTCAGCTGT TTACAATAGA GCAATGGGGC AGTCCTGAGA AACTGCCCAG AGCTCACACA
    TGCTTTAATC GCCTTGACTT ACCTCCATAT GAAACCTTTG AAGATTTACG AGAGAAACTT
    CTCATGGCCG TGGAAAATGC TCAAGGATTT GAAGGGGTGG ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001138443.1
    CDS644..3148
    Translation

    Target ORF information:

    RefSeq Version NM_001144971.2
    Organism Homo sapiens(human)
    Definition Homo sapiens NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant k, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001144971.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGAGCGAC CCTATACATT TAAGGACTTT CTCCTCAGAC CAAGAAGTCA TAAGTCTCGA 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCAA AAAATGGAGG TCAAGATGAA
    GAAAACAGTG ACCAGAGGGA TGACATGGAG CATGGATGGG AAGTTGTTGA CTCAAATGAC
    TCGGCTTCTC AGCACCAAGA GGAACTTCCT CCTCCTCCTC TGCCTCCCGG GTGGGAAGAA
    AAAGTGGACA ATTTAGGCCG AACTTACTAT GTCAACCACA ACAACCGGAC CACTCAGTGG
    CACAGACCAA GCCTGATGGA CGTGTCCTCG GAGTCGGACA ATAACATCAG ACAGATCAAC
    CAGGAGGCAG CACACCGGCG CTTCCGCTCC CGCAGGCACA TCAGCGAAGA CTTGGAGCCC
    GAGCCCTCGG AGGGCGGGGA TGTCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAT
    ATCGCTGGAG ACTCTCTCGG TCTGGCTCTG CCCCCACCAC CGGCCTCCCC AGGATCTCGG
    ACCAGCCCTC AGGAGCTGTC AGAGGAACTA AGCAGAAGGC TTCAGATCAC TCCAGACTCC
    AATGGGGAAC AGTTCAGCTC TTTGATTCAA AGAGAACCCT CCTCAAGGTT GAGGTCATGC
    AGTGTCACCG ACGCAGTTGC AGAACAGGGC CATCTACCAC CGCCATCAGT GGCCTATGTA
    CATACCACGC CGGGTCTGCC TTCAGGCTGG GAAGAAAGAA AAGATGCTAA GGGGCGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GACCTATCAT GCAGCTTGCA
    GAAGATGGTG CGTCCGGATC AGCCACAAAC AGTAACAACC ATCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CTGCCCCGCT GGAGGGTGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GACACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CTTGCCACCC
    GGCTGGGAAA TGAGGATAGC GCCAAACGGC CGGCCCTTCT TCATTGATCA TAACACAAAG
    ACTACAACCT GGGAAGATCC ACGTTTGAAA TTTCCAGTAC ATATGCGGTC AAAGACATCT
    TTAAACCCCA ATGACCTTGG CCCCCTTCCT CCTGGCTGGG AAGAAAGAAT TCACTTGGAT
    GGCCGAACGT TTTATATTGA TCATAATAGC AAAATTACTC AGTGGGAAGA CCCAAGACTG
    CAGAACCCAG CTATTACTGG TCCGGCTGTC CCTTACTCCA GAGAATTTAA GCAGAAATAT
    GACTACTTCA GGAAGAAATT AAAGAAACCT GCTGATATCC CCAATAGGTT TGAAATGAAA
    CTTCACAGAA ATAACATATT TGAAGAGTCC TATCGGAGAA TTATGTCCGT GAAAAGACCA
    GATGTCCTAA AAGCTAGACT GTGGATTGAG TTTGAATCAG AGAAAGGTCT TGACTATGGG
    GGTGTGGCCA GAGAATGGTT CTTCTTACTG TCCAAAGAGA TGTTCAACCC CTACTACGGC
    CTCTTTGAGT ACTCTGCCAC GGACAACTAC ACCCTTCAGA TCAACCCTAA TTCAGGCCTC
    TGTAATGAGG ATCATTTGTC CTACTTCACT TTTATTGGAA GAGTTGCTGG TCTGGCCGTA
    TTTCATGGGA AGCTCTTAGA TGGTTTCTTC ATTAGACCAT TTTACAAGAT GATGTTGGGA
    AAGCAGATAA CCCTGAATGA CATGGAATCT GTGGATAGTG AATATTACAA CTCTTTGAAA
    TGGATCCTGG AGAATGACCC TACTGAGCTG GACCTCATGT TCTGCATAGA CGAAGAAAAC
    TTTGGACAGA CATATCAAGT GGATTTGAAG CCCAATGGGT CAGAAATAAT GGTCACAAAT
    GAAAACAAAA GGGAATATAT CGACTTAGTC ATCCAGTGGA GATTTGTGAA CAGGGTCCAG
    AAGCAGATGA ACGCCTTCTT GGAGGGATTC ACAGAACTAC TTCCTATTGA TTTGATTAAA
    ATTTTTGATG AAAATGAGCT GGAGTTGCTC ATGTGCGGCC TCGGTGATGT GGATGTGAAT
    GACTGGAGAC AGCATTCTAT TTACAAGAAC GGCTACTGCC CAAACCACCC CGTCATTCAG
    TGGTTCTGGA AGGCTGTGCT ACTCATGGAC GCCGAAAAGC GTATCCGGTT ACTGCAGTTT
    GTCACAGGGA CATCGCGAGT ACCTATGAAT GGATTTGCCG AACTTTATGG TTCCAATGGT
    CCTCAGCTGT TTACAATAGA GCAATGGGGC AGTCCTGAGA AACTGCCCAG AGCTCACACA
    TGCTTTAATC GCCTTGACTT ACCTCCATAT GAAACCTTTG AAGATTTACG AGAGAAACTT
    CTCATGGCCG TGGAAAATGC TCAAGGATTT GAAGGGGTGG ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu14254
    Clone ID Related Accession (Same CDS sequence) XM_024451136.1 , XM_024451137.1 , NM_001144971.2 , NM_001144970.3 , NM_001144971.1 , NM_001144970.2 , XM_024451135.1 , XM_024451131.1
    Accession Version NM_001144970.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2505bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-20
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform 6
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA819220.1, AK294963.1, AB007899.1, BC000621.2 and AC090236.4. On May 31, 2019 this sequence version replaced NM_001144970.2. Transcript Variant: This variant (c) uses an alternate first exon and lacks a downstream in-frame exon, compared to variant j. These differences result in the use of a downstream in-frame start codon and a protein (isoform 6) with a shorter N-terminus, compared to isoform 1. Variant c is also known as NEDD4-2B. Variants c and k encode the same isoform (6). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.217257.1, SRR1660805.188278.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGAGCGAC CCTATACATT TAAGGACTTT CTCCTCAGAC CAAGAAGTCA TAAGTCTCGA 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCAA AAAATGGAGG TCAAGATGAA
    GAAAACAGTG ACCAGAGGGA TGACATGGAG CATGGATGGG AAGTTGTTGA CTCAAATGAC
    TCGGCTTCTC AGCACCAAGA GGAACTTCCT CCTCCTCCTC TGCCTCCCGG GTGGGAAGAA
    AAAGTGGACA ATTTAGGCCG AACTTACTAT GTCAACCACA ACAACCGGAC CACTCAGTGG
    CACAGACCAA GCCTGATGGA CGTGTCCTCG GAGTCGGACA ATAACATCAG ACAGATCAAC
    CAGGAGGCAG CACACCGGCG CTTCCGCTCC CGCAGGCACA TCAGCGAAGA CTTGGAGCCC
    GAGCCCTCGG AGGGCGGGGA TGTCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAT
    ATCGCTGGAG ACTCTCTCGG TCTGGCTCTG CCCCCACCAC CGGCCTCCCC AGGATCTCGG
    ACCAGCCCTC AGGAGCTGTC AGAGGAACTA AGCAGAAGGC TTCAGATCAC TCCAGACTCC
    AATGGGGAAC AGTTCAGCTC TTTGATTCAA AGAGAACCCT CCTCAAGGTT GAGGTCATGC
    AGTGTCACCG ACGCAGTTGC AGAACAGGGC CATCTACCAC CGCCATCAGT GGCCTATGTA
    CATACCACGC CGGGTCTGCC TTCAGGCTGG GAAGAAAGAA AAGATGCTAA GGGGCGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GACCTATCAT GCAGCTTGCA
    GAAGATGGTG CGTCCGGATC AGCCACAAAC AGTAACAACC ATCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CTGCCCCGCT GGAGGGTGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GACACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CTTGCCACCC
    GGCTGGGAAA TGAGGATAGC GCCAAACGGC CGGCCCTTCT TCATTGATCA TAACACAAAG
    ACTACAACCT GGGAAGATCC ACGTTTGAAA TTTCCAGTAC ATATGCGGTC AAAGACATCT
    TTAAACCCCA ATGACCTTGG CCCCCTTCCT CCTGGCTGGG AAGAAAGAAT TCACTTGGAT
    GGCCGAACGT TTTATATTGA TCATAATAGC AAAATTACTC AGTGGGAAGA CCCAAGACTG
    CAGAACCCAG CTATTACTGG TCCGGCTGTC CCTTACTCCA GAGAATTTAA GCAGAAATAT
    GACTACTTCA GGAAGAAATT AAAGAAACCT GCTGATATCC CCAATAGGTT TGAAATGAAA
    CTTCACAGAA ATAACATATT TGAAGAGTCC TATCGGAGAA TTATGTCCGT GAAAAGACCA
    GATGTCCTAA AAGCTAGACT GTGGATTGAG TTTGAATCAG AGAAAGGTCT TGACTATGGG
    GGTGTGGCCA GAGAATGGTT CTTCTTACTG TCCAAAGAGA TGTTCAACCC CTACTACGGC
    CTCTTTGAGT ACTCTGCCAC GGACAACTAC ACCCTTCAGA TCAACCCTAA TTCAGGCCTC
    TGTAATGAGG ATCATTTGTC CTACTTCACT TTTATTGGAA GAGTTGCTGG TCTGGCCGTA
    TTTCATGGGA AGCTCTTAGA TGGTTTCTTC ATTAGACCAT TTTACAAGAT GATGTTGGGA
    AAGCAGATAA CCCTGAATGA CATGGAATCT GTGGATAGTG AATATTACAA CTCTTTGAAA
    TGGATCCTGG AGAATGACCC TACTGAGCTG GACCTCATGT TCTGCATAGA CGAAGAAAAC
    TTTGGACAGA CATATCAAGT GGATTTGAAG CCCAATGGGT CAGAAATAAT GGTCACAAAT
    GAAAACAAAA GGGAATATAT CGACTTAGTC ATCCAGTGGA GATTTGTGAA CAGGGTCCAG
    AAGCAGATGA ACGCCTTCTT GGAGGGATTC ACAGAACTAC TTCCTATTGA TTTGATTAAA
    ATTTTTGATG AAAATGAGCT GGAGTTGCTC ATGTGCGGCC TCGGTGATGT GGATGTGAAT
    GACTGGAGAC AGCATTCTAT TTACAAGAAC GGCTACTGCC CAAACCACCC CGTCATTCAG
    TGGTTCTGGA AGGCTGTGCT ACTCATGGAC GCCGAAAAGC GTATCCGGTT ACTGCAGTTT
    GTCACAGGGA CATCGCGAGT ACCTATGAAT GGATTTGCCG AACTTTATGG TTCCAATGGT
    CCTCAGCTGT TTACAATAGA GCAATGGGGC AGTCCTGAGA AACTGCCCAG AGCTCACACA
    TGCTTTAATC GCCTTGACTT ACCTCCATAT GAAACCTTTG AAGATTTACG AGAGAAACTT
    CTCATGGCCG TGGAAAATGC TCAAGGATTT GAAGGGGTGG ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001138442.1
    CDS571..3075
    Translation

    Target ORF information:

    RefSeq Version NM_001144970.3
    Organism Homo sapiens(human)
    Definition Homo sapiens NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant c, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001144970.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGAGCGAC CCTATACATT TAAGGACTTT CTCCTCAGAC CAAGAAGTCA TAAGTCTCGA 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCAA AAAATGGAGG TCAAGATGAA
    GAAAACAGTG ACCAGAGGGA TGACATGGAG CATGGATGGG AAGTTGTTGA CTCAAATGAC
    TCGGCTTCTC AGCACCAAGA GGAACTTCCT CCTCCTCCTC TGCCTCCCGG GTGGGAAGAA
    AAAGTGGACA ATTTAGGCCG AACTTACTAT GTCAACCACA ACAACCGGAC CACTCAGTGG
    CACAGACCAA GCCTGATGGA CGTGTCCTCG GAGTCGGACA ATAACATCAG ACAGATCAAC
    CAGGAGGCAG CACACCGGCG CTTCCGCTCC CGCAGGCACA TCAGCGAAGA CTTGGAGCCC
    GAGCCCTCGG AGGGCGGGGA TGTCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAT
    ATCGCTGGAG ACTCTCTCGG TCTGGCTCTG CCCCCACCAC CGGCCTCCCC AGGATCTCGG
    ACCAGCCCTC AGGAGCTGTC AGAGGAACTA AGCAGAAGGC TTCAGATCAC TCCAGACTCC
    AATGGGGAAC AGTTCAGCTC TTTGATTCAA AGAGAACCCT CCTCAAGGTT GAGGTCATGC
    AGTGTCACCG ACGCAGTTGC AGAACAGGGC CATCTACCAC CGCCATCAGT GGCCTATGTA
    CATACCACGC CGGGTCTGCC TTCAGGCTGG GAAGAAAGAA AAGATGCTAA GGGGCGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GACCTATCAT GCAGCTTGCA
    GAAGATGGTG CGTCCGGATC AGCCACAAAC AGTAACAACC ATCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CTGCCCCGCT GGAGGGTGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GACACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CTTGCCACCC
    GGCTGGGAAA TGAGGATAGC GCCAAACGGC CGGCCCTTCT TCATTGATCA TAACACAAAG
    ACTACAACCT GGGAAGATCC ACGTTTGAAA TTTCCAGTAC ATATGCGGTC AAAGACATCT
    TTAAACCCCA ATGACCTTGG CCCCCTTCCT CCTGGCTGGG AAGAAAGAAT TCACTTGGAT
    GGCCGAACGT TTTATATTGA TCATAATAGC AAAATTACTC AGTGGGAAGA CCCAAGACTG
    CAGAACCCAG CTATTACTGG TCCGGCTGTC CCTTACTCCA GAGAATTTAA GCAGAAATAT
    GACTACTTCA GGAAGAAATT AAAGAAACCT GCTGATATCC CCAATAGGTT TGAAATGAAA
    CTTCACAGAA ATAACATATT TGAAGAGTCC TATCGGAGAA TTATGTCCGT GAAAAGACCA
    GATGTCCTAA AAGCTAGACT GTGGATTGAG TTTGAATCAG AGAAAGGTCT TGACTATGGG
    GGTGTGGCCA GAGAATGGTT CTTCTTACTG TCCAAAGAGA TGTTCAACCC CTACTACGGC
    CTCTTTGAGT ACTCTGCCAC GGACAACTAC ACCCTTCAGA TCAACCCTAA TTCAGGCCTC
    TGTAATGAGG ATCATTTGTC CTACTTCACT TTTATTGGAA GAGTTGCTGG TCTGGCCGTA
    TTTCATGGGA AGCTCTTAGA TGGTTTCTTC ATTAGACCAT TTTACAAGAT GATGTTGGGA
    AAGCAGATAA CCCTGAATGA CATGGAATCT GTGGATAGTG AATATTACAA CTCTTTGAAA
    TGGATCCTGG AGAATGACCC TACTGAGCTG GACCTCATGT TCTGCATAGA CGAAGAAAAC
    TTTGGACAGA CATATCAAGT GGATTTGAAG CCCAATGGGT CAGAAATAAT GGTCACAAAT
    GAAAACAAAA GGGAATATAT CGACTTAGTC ATCCAGTGGA GATTTGTGAA CAGGGTCCAG
    AAGCAGATGA ACGCCTTCTT GGAGGGATTC ACAGAACTAC TTCCTATTGA TTTGATTAAA
    ATTTTTGATG AAAATGAGCT GGAGTTGCTC ATGTGCGGCC TCGGTGATGT GGATGTGAAT
    GACTGGAGAC AGCATTCTAT TTACAAGAAC GGCTACTGCC CAAACCACCC CGTCATTCAG
    TGGTTCTGGA AGGCTGTGCT ACTCATGGAC GCCGAAAAGC GTATCCGGTT ACTGCAGTTT
    GTCACAGGGA CATCGCGAGT ACCTATGAAT GGATTTGCCG AACTTTATGG TTCCAATGGT
    CCTCAGCTGT TTACAATAGA GCAATGGGGC AGTCCTGAGA AACTGCCCAG AGCTCACACA
    TGCTTTAATC GCCTTGACTT ACCTCCATAT GAAACCTTTG AAGATTTACG AGAGAAACTT
    CTCATGGCCG TGGAAAATGC TCAAGGATTT GAAGGGGTGG ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu14254
    Clone ID Related Accession (Same CDS sequence) XM_024451136.1 , XM_024451137.1 , NM_001144971.2 , NM_001144970.3 , NM_001144971.1 , NM_001144970.2 , XM_024451135.1 , XM_024451131.1
    Accession Version NM_001144971.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2505bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-03
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform 6
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DQ181796.1, BP260852.1, AF070601.1, DA759940.1, AC090236.4 and DB317092.1. Transcript Variant: This variant (k) uses two alternate exons in its 5' end and lacks a downstream in-frame exon, compared to variant j. These differences result in the use of a downstream in-frame start codon and a protein (isoform 6) with a shorter N-terminus, compared to isoform 1. Variants c and k encode the same isoform (6). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ181796.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGAGCGAC CCTATACATT TAAGGACTTT CTCCTCAGAC CAAGAAGTCA TAAGTCTCGA 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCAA AAAATGGAGG TCAAGATGAA
    GAAAACAGTG ACCAGAGGGA TGACATGGAG CATGGATGGG AAGTTGTTGA CTCAAATGAC
    TCGGCTTCTC AGCACCAAGA GGAACTTCCT CCTCCTCCTC TGCCTCCCGG GTGGGAAGAA
    AAAGTGGACA ATTTAGGCCG AACTTACTAT GTCAACCACA ACAACCGGAC CACTCAGTGG
    CACAGACCAA GCCTGATGGA CGTGTCCTCG GAGTCGGACA ATAACATCAG ACAGATCAAC
    CAGGAGGCAG CACACCGGCG CTTCCGCTCC CGCAGGCACA TCAGCGAAGA CTTGGAGCCC
    GAGCCCTCGG AGGGCGGGGA TGTCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAT
    ATCGCTGGAG ACTCTCTCGG TCTGGCTCTG CCCCCACCAC CGGCCTCCCC AGGATCTCGG
    ACCAGCCCTC AGGAGCTGTC AGAGGAACTA AGCAGAAGGC TTCAGATCAC TCCAGACTCC
    AATGGGGAAC AGTTCAGCTC TTTGATTCAA AGAGAACCCT CCTCAAGGTT GAGGTCATGC
    AGTGTCACCG ACGCAGTTGC AGAACAGGGC CATCTACCAC CGCCATCAGT GGCCTATGTA
    CATACCACGC CGGGTCTGCC TTCAGGCTGG GAAGAAAGAA AAGATGCTAA GGGGCGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GACCTATCAT GCAGCTTGCA
    GAAGATGGTG CGTCCGGATC AGCCACAAAC AGTAACAACC ATCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CTGCCCCGCT GGAGGGTGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GACACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CTTGCCACCC
    GGCTGGGAAA TGAGGATAGC GCCAAACGGC CGGCCCTTCT TCATTGATCA TAACACAAAG
    ACTACAACCT GGGAAGATCC ACGTTTGAAA TTTCCAGTAC ATATGCGGTC AAAGACATCT
    TTAAACCCCA ATGACCTTGG CCCCCTTCCT CCTGGCTGGG AAGAAAGAAT TCACTTGGAT
    GGCCGAACGT TTTATATTGA TCATAATAGC AAAATTACTC AGTGGGAAGA CCCAAGACTG
    CAGAACCCAG CTATTACTGG TCCGGCTGTC CCTTACTCCA GAGAATTTAA GCAGAAATAT
    GACTACTTCA GGAAGAAATT AAAGAAACCT GCTGATATCC CCAATAGGTT TGAAATGAAA
    CTTCACAGAA ATAACATATT TGAAGAGTCC TATCGGAGAA TTATGTCCGT GAAAAGACCA
    GATGTCCTAA AAGCTAGACT GTGGATTGAG TTTGAATCAG AGAAAGGTCT TGACTATGGG
    GGTGTGGCCA GAGAATGGTT CTTCTTACTG TCCAAAGAGA TGTTCAACCC CTACTACGGC
    CTCTTTGAGT ACTCTGCCAC GGACAACTAC ACCCTTCAGA TCAACCCTAA TTCAGGCCTC
    TGTAATGAGG ATCATTTGTC CTACTTCACT TTTATTGGAA GAGTTGCTGG TCTGGCCGTA
    TTTCATGGGA AGCTCTTAGA TGGTTTCTTC ATTAGACCAT TTTACAAGAT GATGTTGGGA
    AAGCAGATAA CCCTGAATGA CATGGAATCT GTGGATAGTG AATATTACAA CTCTTTGAAA
    TGGATCCTGG AGAATGACCC TACTGAGCTG GACCTCATGT TCTGCATAGA CGAAGAAAAC
    TTTGGACAGA CATATCAAGT GGATTTGAAG CCCAATGGGT CAGAAATAAT GGTCACAAAT
    GAAAACAAAA GGGAATATAT CGACTTAGTC ATCCAGTGGA GATTTGTGAA CAGGGTCCAG
    AAGCAGATGA ACGCCTTCTT GGAGGGATTC ACAGAACTAC TTCCTATTGA TTTGATTAAA
    ATTTTTGATG AAAATGAGCT GGAGTTGCTC ATGTGCGGCC TCGGTGATGT GGATGTGAAT
    GACTGGAGAC AGCATTCTAT TTACAAGAAC GGCTACTGCC CAAACCACCC CGTCATTCAG
    TGGTTCTGGA AGGCTGTGCT ACTCATGGAC GCCGAAAAGC GTATCCGGTT ACTGCAGTTT
    GTCACAGGGA CATCGCGAGT ACCTATGAAT GGATTTGCCG AACTTTATGG TTCCAATGGT
    CCTCAGCTGT TTACAATAGA GCAATGGGGC AGTCCTGAGA AACTGCCCAG AGCTCACACA
    TGCTTTAATC GCCTTGACTT ACCTCCATAT GAAACCTTTG AAGATTTACG AGAGAAACTT
    CTCATGGCCG TGGAAAATGC TCAAGGATTT GAAGGGGTGG ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001138443.1
    CDS631..3135
    Translation

    Target ORF information:

    RefSeq Version NM_001144971.1
    Organism Homo sapiens(human)
    Definition Homo sapiens neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant k, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001144971.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGAGCGAC CCTATACATT TAAGGACTTT CTCCTCAGAC CAAGAAGTCA TAAGTCTCGA 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCAA AAAATGGAGG TCAAGATGAA
    GAAAACAGTG ACCAGAGGGA TGACATGGAG CATGGATGGG AAGTTGTTGA CTCAAATGAC
    TCGGCTTCTC AGCACCAAGA GGAACTTCCT CCTCCTCCTC TGCCTCCCGG GTGGGAAGAA
    AAAGTGGACA ATTTAGGCCG AACTTACTAT GTCAACCACA ACAACCGGAC CACTCAGTGG
    CACAGACCAA GCCTGATGGA CGTGTCCTCG GAGTCGGACA ATAACATCAG ACAGATCAAC
    CAGGAGGCAG CACACCGGCG CTTCCGCTCC CGCAGGCACA TCAGCGAAGA CTTGGAGCCC
    GAGCCCTCGG AGGGCGGGGA TGTCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAT
    ATCGCTGGAG ACTCTCTCGG TCTGGCTCTG CCCCCACCAC CGGCCTCCCC AGGATCTCGG
    ACCAGCCCTC AGGAGCTGTC AGAGGAACTA AGCAGAAGGC TTCAGATCAC TCCAGACTCC
    AATGGGGAAC AGTTCAGCTC TTTGATTCAA AGAGAACCCT CCTCAAGGTT GAGGTCATGC
    AGTGTCACCG ACGCAGTTGC AGAACAGGGC CATCTACCAC CGCCATCAGT GGCCTATGTA
    CATACCACGC CGGGTCTGCC TTCAGGCTGG GAAGAAAGAA AAGATGCTAA GGGGCGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GACCTATCAT GCAGCTTGCA
    GAAGATGGTG CGTCCGGATC AGCCACAAAC AGTAACAACC ATCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CTGCCCCGCT GGAGGGTGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GACACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CTTGCCACCC
    GGCTGGGAAA TGAGGATAGC GCCAAACGGC CGGCCCTTCT TCATTGATCA TAACACAAAG
    ACTACAACCT GGGAAGATCC ACGTTTGAAA TTTCCAGTAC ATATGCGGTC AAAGACATCT
    TTAAACCCCA ATGACCTTGG CCCCCTTCCT CCTGGCTGGG AAGAAAGAAT TCACTTGGAT
    GGCCGAACGT TTTATATTGA TCATAATAGC AAAATTACTC AGTGGGAAGA CCCAAGACTG
    CAGAACCCAG CTATTACTGG TCCGGCTGTC CCTTACTCCA GAGAATTTAA GCAGAAATAT
    GACTACTTCA GGAAGAAATT AAAGAAACCT GCTGATATCC CCAATAGGTT TGAAATGAAA
    CTTCACAGAA ATAACATATT TGAAGAGTCC TATCGGAGAA TTATGTCCGT GAAAAGACCA
    GATGTCCTAA AAGCTAGACT GTGGATTGAG TTTGAATCAG AGAAAGGTCT TGACTATGGG
    GGTGTGGCCA GAGAATGGTT CTTCTTACTG TCCAAAGAGA TGTTCAACCC CTACTACGGC
    CTCTTTGAGT ACTCTGCCAC GGACAACTAC ACCCTTCAGA TCAACCCTAA TTCAGGCCTC
    TGTAATGAGG ATCATTTGTC CTACTTCACT TTTATTGGAA GAGTTGCTGG TCTGGCCGTA
    TTTCATGGGA AGCTCTTAGA TGGTTTCTTC ATTAGACCAT TTTACAAGAT GATGTTGGGA
    AAGCAGATAA CCCTGAATGA CATGGAATCT GTGGATAGTG AATATTACAA CTCTTTGAAA
    TGGATCCTGG AGAATGACCC TACTGAGCTG GACCTCATGT TCTGCATAGA CGAAGAAAAC
    TTTGGACAGA CATATCAAGT GGATTTGAAG CCCAATGGGT CAGAAATAAT GGTCACAAAT
    GAAAACAAAA GGGAATATAT CGACTTAGTC ATCCAGTGGA GATTTGTGAA CAGGGTCCAG
    AAGCAGATGA ACGCCTTCTT GGAGGGATTC ACAGAACTAC TTCCTATTGA TTTGATTAAA
    ATTTTTGATG AAAATGAGCT GGAGTTGCTC ATGTGCGGCC TCGGTGATGT GGATGTGAAT
    GACTGGAGAC AGCATTCTAT TTACAAGAAC GGCTACTGCC CAAACCACCC CGTCATTCAG
    TGGTTCTGGA AGGCTGTGCT ACTCATGGAC GCCGAAAAGC GTATCCGGTT ACTGCAGTTT
    GTCACAGGGA CATCGCGAGT ACCTATGAAT GGATTTGCCG AACTTTATGG TTCCAATGGT
    CCTCAGCTGT TTACAATAGA GCAATGGGGC AGTCCTGAGA AACTGCCCAG AGCTCACACA
    TGCTTTAATC GCCTTGACTT ACCTCCATAT GAAACCTTTG AAGATTTACG AGAGAAACTT
    CTCATGGCCG TGGAAAATGC TCAAGGATTT GAAGGGGTGG ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu14254
    Clone ID Related Accession (Same CDS sequence) XM_024451136.1 , XM_024451137.1 , NM_001144971.2 , NM_001144970.3 , NM_001144971.1 , NM_001144970.2 , XM_024451135.1 , XM_024451131.1
    Accession Version NM_001144970.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2505bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-03
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform 6
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA819220.1, AK294963.1, AB007899.1, BC000621.2 and AC090236.4. On Sep 3, 2011 this sequence version replaced NM_001144970.1. Transcript Variant: This variant (c) uses an alternate first exon and lacks a downstream in-frame exon, compared to variant j. These differences result in the use of a downstream in-frame start codon and a protein (isoform 6) with a shorter N-terminus, compared to isoform 1. Variant c is also known as NEDD4-2B. Variants c and k encode the same isoform (6). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.263039.1, SRR1803611.108088.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGAGCGAC CCTATACATT TAAGGACTTT CTCCTCAGAC CAAGAAGTCA TAAGTCTCGA 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCAA AAAATGGAGG TCAAGATGAA
    GAAAACAGTG ACCAGAGGGA TGACATGGAG CATGGATGGG AAGTTGTTGA CTCAAATGAC
    TCGGCTTCTC AGCACCAAGA GGAACTTCCT CCTCCTCCTC TGCCTCCCGG GTGGGAAGAA
    AAAGTGGACA ATTTAGGCCG AACTTACTAT GTCAACCACA ACAACCGGAC CACTCAGTGG
    CACAGACCAA GCCTGATGGA CGTGTCCTCG GAGTCGGACA ATAACATCAG ACAGATCAAC
    CAGGAGGCAG CACACCGGCG CTTCCGCTCC CGCAGGCACA TCAGCGAAGA CTTGGAGCCC
    GAGCCCTCGG AGGGCGGGGA TGTCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAT
    ATCGCTGGAG ACTCTCTCGG TCTGGCTCTG CCCCCACCAC CGGCCTCCCC AGGATCTCGG
    ACCAGCCCTC AGGAGCTGTC AGAGGAACTA AGCAGAAGGC TTCAGATCAC TCCAGACTCC
    AATGGGGAAC AGTTCAGCTC TTTGATTCAA AGAGAACCCT CCTCAAGGTT GAGGTCATGC
    AGTGTCACCG ACGCAGTTGC AGAACAGGGC CATCTACCAC CGCCATCAGT GGCCTATGTA
    CATACCACGC CGGGTCTGCC TTCAGGCTGG GAAGAAAGAA AAGATGCTAA GGGGCGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GACCTATCAT GCAGCTTGCA
    GAAGATGGTG CGTCCGGATC AGCCACAAAC AGTAACAACC ATCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CTGCCCCGCT GGAGGGTGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GACACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CTTGCCACCC
    GGCTGGGAAA TGAGGATAGC GCCAAACGGC CGGCCCTTCT TCATTGATCA TAACACAAAG
    ACTACAACCT GGGAAGATCC ACGTTTGAAA TTTCCAGTAC ATATGCGGTC AAAGACATCT
    TTAAACCCCA ATGACCTTGG CCCCCTTCCT CCTGGCTGGG AAGAAAGAAT TCACTTGGAT
    GGCCGAACGT TTTATATTGA TCATAATAGC AAAATTACTC AGTGGGAAGA CCCAAGACTG
    CAGAACCCAG CTATTACTGG TCCGGCTGTC CCTTACTCCA GAGAATTTAA GCAGAAATAT
    GACTACTTCA GGAAGAAATT AAAGAAACCT GCTGATATCC CCAATAGGTT TGAAATGAAA
    CTTCACAGAA ATAACATATT TGAAGAGTCC TATCGGAGAA TTATGTCCGT GAAAAGACCA
    GATGTCCTAA AAGCTAGACT GTGGATTGAG TTTGAATCAG AGAAAGGTCT TGACTATGGG
    GGTGTGGCCA GAGAATGGTT CTTCTTACTG TCCAAAGAGA TGTTCAACCC CTACTACGGC
    CTCTTTGAGT ACTCTGCCAC GGACAACTAC ACCCTTCAGA TCAACCCTAA TTCAGGCCTC
    TGTAATGAGG ATCATTTGTC CTACTTCACT TTTATTGGAA GAGTTGCTGG TCTGGCCGTA
    TTTCATGGGA AGCTCTTAGA TGGTTTCTTC ATTAGACCAT TTTACAAGAT GATGTTGGGA
    AAGCAGATAA CCCTGAATGA CATGGAATCT GTGGATAGTG AATATTACAA CTCTTTGAAA
    TGGATCCTGG AGAATGACCC TACTGAGCTG GACCTCATGT TCTGCATAGA CGAAGAAAAC
    TTTGGACAGA CATATCAAGT GGATTTGAAG CCCAATGGGT CAGAAATAAT GGTCACAAAT
    GAAAACAAAA GGGAATATAT CGACTTAGTC ATCCAGTGGA GATTTGTGAA CAGGGTCCAG
    AAGCAGATGA ACGCCTTCTT GGAGGGATTC ACAGAACTAC TTCCTATTGA TTTGATTAAA
    ATTTTTGATG AAAATGAGCT GGAGTTGCTC ATGTGCGGCC TCGGTGATGT GGATGTGAAT
    GACTGGAGAC AGCATTCTAT TTACAAGAAC GGCTACTGCC CAAACCACCC CGTCATTCAG
    TGGTTCTGGA AGGCTGTGCT ACTCATGGAC GCCGAAAAGC GTATCCGGTT ACTGCAGTTT
    GTCACAGGGA CATCGCGAGT ACCTATGAAT GGATTTGCCG AACTTTATGG TTCCAATGGT
    CCTCAGCTGT TTACAATAGA GCAATGGGGC AGTCCTGAGA AACTGCCCAG AGCTCACACA
    TGCTTTAATC GCCTTGACTT ACCTCCATAT GAAACCTTTG AAGATTTACG AGAGAAACTT
    CTCATGGCCG TGGAAAATGC TCAAGGATTT GAAGGGGTGG ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001138442.1
    CDS586..3090
    Translation

    Target ORF information:

    RefSeq Version NM_001144970.2
    Organism Homo sapiens(human)
    Definition Homo sapiens neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant c, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001144970.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGAGCGAC CCTATACATT TAAGGACTTT CTCCTCAGAC CAAGAAGTCA TAAGTCTCGA 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCAA AAAATGGAGG TCAAGATGAA
    GAAAACAGTG ACCAGAGGGA TGACATGGAG CATGGATGGG AAGTTGTTGA CTCAAATGAC
    TCGGCTTCTC AGCACCAAGA GGAACTTCCT CCTCCTCCTC TGCCTCCCGG GTGGGAAGAA
    AAAGTGGACA ATTTAGGCCG AACTTACTAT GTCAACCACA ACAACCGGAC CACTCAGTGG
    CACAGACCAA GCCTGATGGA CGTGTCCTCG GAGTCGGACA ATAACATCAG ACAGATCAAC
    CAGGAGGCAG CACACCGGCG CTTCCGCTCC CGCAGGCACA TCAGCGAAGA CTTGGAGCCC
    GAGCCCTCGG AGGGCGGGGA TGTCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAT
    ATCGCTGGAG ACTCTCTCGG TCTGGCTCTG CCCCCACCAC CGGCCTCCCC AGGATCTCGG
    ACCAGCCCTC AGGAGCTGTC AGAGGAACTA AGCAGAAGGC TTCAGATCAC TCCAGACTCC
    AATGGGGAAC AGTTCAGCTC TTTGATTCAA AGAGAACCCT CCTCAAGGTT GAGGTCATGC
    AGTGTCACCG ACGCAGTTGC AGAACAGGGC CATCTACCAC CGCCATCAGT GGCCTATGTA
    CATACCACGC CGGGTCTGCC TTCAGGCTGG GAAGAAAGAA AAGATGCTAA GGGGCGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GACCTATCAT GCAGCTTGCA
    GAAGATGGTG CGTCCGGATC AGCCACAAAC AGTAACAACC ATCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CTGCCCCGCT GGAGGGTGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GACACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CTTGCCACCC
    GGCTGGGAAA TGAGGATAGC GCCAAACGGC CGGCCCTTCT TCATTGATCA TAACACAAAG
    ACTACAACCT GGGAAGATCC ACGTTTGAAA TTTCCAGTAC ATATGCGGTC AAAGACATCT
    TTAAACCCCA ATGACCTTGG CCCCCTTCCT CCTGGCTGGG AAGAAAGAAT TCACTTGGAT
    GGCCGAACGT TTTATATTGA TCATAATAGC AAAATTACTC AGTGGGAAGA CCCAAGACTG
    CAGAACCCAG CTATTACTGG TCCGGCTGTC CCTTACTCCA GAGAATTTAA GCAGAAATAT
    GACTACTTCA GGAAGAAATT AAAGAAACCT GCTGATATCC CCAATAGGTT TGAAATGAAA
    CTTCACAGAA ATAACATATT TGAAGAGTCC TATCGGAGAA TTATGTCCGT GAAAAGACCA
    GATGTCCTAA AAGCTAGACT GTGGATTGAG TTTGAATCAG AGAAAGGTCT TGACTATGGG
    GGTGTGGCCA GAGAATGGTT CTTCTTACTG TCCAAAGAGA TGTTCAACCC CTACTACGGC
    CTCTTTGAGT ACTCTGCCAC GGACAACTAC ACCCTTCAGA TCAACCCTAA TTCAGGCCTC
    TGTAATGAGG ATCATTTGTC CTACTTCACT TTTATTGGAA GAGTTGCTGG TCTGGCCGTA
    TTTCATGGGA AGCTCTTAGA TGGTTTCTTC ATTAGACCAT TTTACAAGAT GATGTTGGGA
    AAGCAGATAA CCCTGAATGA CATGGAATCT GTGGATAGTG AATATTACAA CTCTTTGAAA
    TGGATCCTGG AGAATGACCC TACTGAGCTG GACCTCATGT TCTGCATAGA CGAAGAAAAC
    TTTGGACAGA CATATCAAGT GGATTTGAAG CCCAATGGGT CAGAAATAAT GGTCACAAAT
    GAAAACAAAA GGGAATATAT CGACTTAGTC ATCCAGTGGA GATTTGTGAA CAGGGTCCAG
    AAGCAGATGA ACGCCTTCTT GGAGGGATTC ACAGAACTAC TTCCTATTGA TTTGATTAAA
    ATTTTTGATG AAAATGAGCT GGAGTTGCTC ATGTGCGGCC TCGGTGATGT GGATGTGAAT
    GACTGGAGAC AGCATTCTAT TTACAAGAAC GGCTACTGCC CAAACCACCC CGTCATTCAG
    TGGTTCTGGA AGGCTGTGCT ACTCATGGAC GCCGAAAAGC GTATCCGGTT ACTGCAGTTT
    GTCACAGGGA CATCGCGAGT ACCTATGAAT GGATTTGCCG AACTTTATGG TTCCAATGGT
    CCTCAGCTGT TTACAATAGA GCAATGGGGC AGTCCTGAGA AACTGCCCAG AGCTCACACA
    TGCTTTAATC GCCTTGACTT ACCTCCATAT GAAACCTTTG AAGATTTACG AGAGAAACTT
    CTCATGGCCG TGGAAAATGC TCAAGGATTT GAAGGGGTGG ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu14336
    Clone ID Related Accession (Same CDS sequence) NM_001144968.1 , NM_001144968.2
    Accession Version NM_001144968.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2904bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-03
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform 4
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY112984.2, BC032597.2 and AC090236.4. Transcript Variant: This variant (g) uses an alternate first exon, compared to variant j, which results in the use of a different start codon and a protein (isoform 4) with a shorter and distinct N-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript was derived from the reference genome assembly. The genomic coordinates were determined from alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY112984.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAGACGTC TCGCATTTGA GCAGGGAGAG TCCCGTATTC TCAGAGTAAA AGTTGTTTCT 
    GGAATTGATC TCGCCAAAAA GGACATCTTT GGAGCCAGTG ATCCGTATGT GAAACTTTCA
    TTGTACGTAG CGGATGAGAA TAGAGAACTT GCTTTGGTCC AGACAAAAAC AATTAAAAAG
    ACACTGAACC CAAAATGGAA TGAAGAATTT TATTTCAGGG TAAACCCATC TAATCACAGA
    CTCCTATTTG AAGTATTTGA CGAAAATAGA CTGACACGAG ACGACTTCCT GGGCCAGGTG
    GACGTGCCCC TTAGTCACCT TCCGACAGAA GATCCAACCA TGGAGCGACC CTATACATTT
    AAGGACTTTC TCCTCAGACC AAGAAGTCAT AAGTCTCGAG TTAAGGGATT TTTGCGATTG
    AAAATGGCCT ATATGCCAAA AAATGGAGGT CAAGATGAAG AAAACAGTGA CCAGAGGGAT
    GACATGGAGC ATGGATGGGA AGTTGTTGAC TCAAATGACT CGGCTTCTCA GCACCAAGAG
    GAACTTCCTC CTCCTCCTCT GCCTCCCGGG TGGGAAGAAA AAGTGGACAA TTTAGGCCGA
    ACTTACTATG TCAACCACAA CAACCGGACC ACTCAGTGGC ACAGACCAAG CCTGATGGAC
    GTGTCCTCGG AGTCGGACAA TAACATCAGA CAGATCAACC AGGAGGCAGC ACACCGGCGC
    TTCCGCTCCC GCAGGCACAT CAGCGAAGAC TTGGAGCCCG AGCCCTCGGA GGGCGGGGAT
    GTCCCCGAGC CTTGGGAGAC CATTTCAGAG GAAGTGAATA TCGCTGGAGA CTCTCTCGGT
    CTGGCTCTGC CCCCACCACC GGCCTCCCCA GGATCTCGGA CCAGCCCTCA GGAGCTGTCA
    GAGGAACTAA GCAGAAGGCT TCAGATCACT CCAGACTCCA ATGGGGAACA GTTCAGCTCT
    TTGATTCAAA GAGAACCCTC CTCAAGGTTG AGGTCATGCA GTGTCACCGA CGCAGTTGCA
    GAACAGGGCC ATCTACCACC GCCCAGTGCC CCAGCTGGGA GAGCGCGTTC ATCAACTGTC
    ACGGGTGGTG AGGAACCAAC GCCATCAGTG GCCTATGTAC ATACCACGCC GGGTCTGCCT
    TCAGGCTGGG AAGAAAGAAA AGATGCTAAG GGGCGCACAT ACTATGTCAA TCATAACAAT
    CGAACCACAA CTTGGACTCG ACCTATCATG CAGCTTGCAG AAGATGGTGC GTCCGGATCA
    GCCACAAACA GTAACAACCA TCTAATCGAG CCTCAGATCC GCCGGCCTCG TAGCCTCAGC
    TCGCCAACAG TAACTTTATC TGCCCCGCTG GAGGGTGCCA AGGACTCACC CGTACGTCGG
    GCTGTGAAAG ACACCCTTTC CAACCCACAG TCCCCACAGC CATCACCTTA CAACTCCCCC
    AAACCACAAC ACAAAGTCAC ACAGAGCTTC TTGCCACCCG GCTGGGAAAT GAGGATAGCG
    CCAAACGGCC GGCCCTTCTT CATTGATCAT AACACAAAGA CTACAACCTG GGAAGATCCA
    CGTTTGAAAT TTCCAGTACA TATGCGGTCA AAGACATCTT TAAACCCCAA TGACCTTGGC
    CCCCTTCCTC CTGGCTGGGA AGAAAGAATT CACTTGGATG GCCGAACGTT TTATATTGAT
    CATAATAGCA AAATTACTCA GTGGGAAGAC CCAAGACTGC AGAACCCAGC TATTACTGGT
    CCGGCTGTCC CTTACTCCAG AGAATTTAAG CAGAAATATG ACTACTTCAG GAAGAAATTA
    AAGAAACCTG CTGATATCCC CAATAGGTTT GAAATGAAAC TTCACAGAAA TAACATATTT
    GAAGAGTCCT ATCGGAGAAT TATGTCCGTG AAAAGACCAG ATGTCCTAAA AGCTAGACTG
    TGGATTGAGT TTGAATCAGA GAAAGGTCTT GACTATGGGG GTGTGGCCAG AGAATGGTTC
    TTCTTACTGT CCAAAGAGAT GTTCAACCCC TACTACGGCC TCTTTGAGTA CTCTGCCACG
    GACAACTACA CCCTTCAGAT CAACCCTAAT TCAGGCCTCT GTAATGAGGA TCATTTGTCC
    TACTTCACTT TTATTGGAAG AGTTGCTGGT CTGGCCGTAT TTCATGGGAA GCTCTTAGAT
    GGTTTCTTCA TTAGACCATT TTACAAGATG ATGTTGGGAA AGCAGATAAC CCTGAATGAC
    ATGGAATCTG TGGATAGTGA ATATTACAAC TCTTTGAAAT GGATCCTGGA GAATGACCCT
    ACTGAGCTGG ACCTCATGTT CTGCATAGAC GAAGAAAACT TTGGACAGAC ATATCAAGTG
    GATTTGAAGC CCAATGGGTC AGAAATAATG GTCACAAATG AAAACAAAAG GGAATATATC
    GACTTAGTCA TCCAGTGGAG ATTTGTGAAC AGGGTCCAGA AGCAGATGAA CGCCTTCTTG
    GAGGGATTCA CAGAACTACT TCCTATTGAT TTGATTAAAA TTTTTGATGA AAATGAGCTG
    GAGTTGCTCA TGTGCGGCCT CGGTGATGTG GATGTGAATG ACTGGAGACA GCATTCTATT
    TACAAGAACG GCTACTGCCC AAACCACCCC GTCATTCAGT GGTTCTGGAA GGCTGTGCTA
    CTCATGGACG CCGAAAAGCG TATCCGGTTA CTGCAGTTTG TCACAGGGAC ATCGCGAGTA
    CCTATGAATG GATTTGCCGA ACTTTATGGT TCCAATGGTC CTCAGCTGTT TACAATAGAG
    CAATGGGGCA GTCCTGAGAA ACTGCCCAGA GCTCACACAT GCTTTAATCG CCTTGACTTA
    CCTCCATATG AAACCTTTGA AGATTTACGA GAGAAACTTC TCATGGCCGT GGAAAATGCT
    CAAGGATTTG AAGGGGTGGA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001138440.1
    CDS204..3107
    Translation

    Target ORF information:

    RefSeq Version NM_001144968.1
    Organism Homo sapiens(human)
    Definition Homo sapiens neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant g, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001144968.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAGACGTC TCGCATTTGA GCAGGGAGAG TCCCGTATTC TCAGAGTAAA AGTTGTTTCT 
    GGAATTGATC TCGCCAAAAA GGACATCTTT GGAGCCAGTG ATCCGTATGT GAAACTTTCA
    TTGTACGTAG CGGATGAGAA TAGAGAACTT GCTTTGGTCC AGACAAAAAC AATTAAAAAG
    ACACTGAACC CAAAATGGAA TGAAGAATTT TATTTCAGGG TAAACCCATC TAATCACAGA
    CTCCTATTTG AAGTATTTGA CGAAAATAGA CTGACACGAG ACGACTTCCT GGGCCAGGTG
    GACGTGCCCC TTAGTCACCT TCCGACAGAA GATCCAACCA TGGAGCGACC CTATACATTT
    AAGGACTTTC TCCTCAGACC AAGAAGTCAT AAGTCTCGAG TTAAGGGATT TTTGCGATTG
    AAAATGGCCT ATATGCCAAA AAATGGAGGT CAAGATGAAG AAAACAGTGA CCAGAGGGAT
    GACATGGAGC ATGGATGGGA AGTTGTTGAC TCAAATGACT CGGCTTCTCA GCACCAAGAG
    GAACTTCCTC CTCCTCCTCT GCCTCCCGGG TGGGAAGAAA AAGTGGACAA TTTAGGCCGA
    ACTTACTATG TCAACCACAA CAACCGGACC ACTCAGTGGC ACAGACCAAG CCTGATGGAC
    GTGTCCTCGG AGTCGGACAA TAACATCAGA CAGATCAACC AGGAGGCAGC ACACCGGCGC
    TTCCGCTCCC GCAGGCACAT CAGCGAAGAC TTGGAGCCCG AGCCCTCGGA GGGCGGGGAT
    GTCCCCGAGC CTTGGGAGAC CATTTCAGAG GAAGTGAATA TCGCTGGAGA CTCTCTCGGT
    CTGGCTCTGC CCCCACCACC GGCCTCCCCA GGATCTCGGA CCAGCCCTCA GGAGCTGTCA
    GAGGAACTAA GCAGAAGGCT TCAGATCACT CCAGACTCCA ATGGGGAACA GTTCAGCTCT
    TTGATTCAAA GAGAACCCTC CTCAAGGTTG AGGTCATGCA GTGTCACCGA CGCAGTTGCA
    GAACAGGGCC ATCTACCACC GCCCAGTGCC CCAGCTGGGA GAGCGCGTTC ATCAACTGTC
    ACGGGTGGTG AGGAACCAAC GCCATCAGTG GCCTATGTAC ATACCACGCC GGGTCTGCCT
    TCAGGCTGGG AAGAAAGAAA AGATGCTAAG GGGCGCACAT ACTATGTCAA TCATAACAAT
    CGAACCACAA CTTGGACTCG ACCTATCATG CAGCTTGCAG AAGATGGTGC GTCCGGATCA
    GCCACAAACA GTAACAACCA TCTAATCGAG CCTCAGATCC GCCGGCCTCG TAGCCTCAGC
    TCGCCAACAG TAACTTTATC TGCCCCGCTG GAGGGTGCCA AGGACTCACC CGTACGTCGG
    GCTGTGAAAG ACACCCTTTC CAACCCACAG TCCCCACAGC CATCACCTTA CAACTCCCCC
    AAACCACAAC ACAAAGTCAC ACAGAGCTTC TTGCCACCCG GCTGGGAAAT GAGGATAGCG
    CCAAACGGCC GGCCCTTCTT CATTGATCAT AACACAAAGA CTACAACCTG GGAAGATCCA
    CGTTTGAAAT TTCCAGTACA TATGCGGTCA AAGACATCTT TAAACCCCAA TGACCTTGGC
    CCCCTTCCTC CTGGCTGGGA AGAAAGAATT CACTTGGATG GCCGAACGTT TTATATTGAT
    CATAATAGCA AAATTACTCA GTGGGAAGAC CCAAGACTGC AGAACCCAGC TATTACTGGT
    CCGGCTGTCC CTTACTCCAG AGAATTTAAG CAGAAATATG ACTACTTCAG GAAGAAATTA
    AAGAAACCTG CTGATATCCC CAATAGGTTT GAAATGAAAC TTCACAGAAA TAACATATTT
    GAAGAGTCCT ATCGGAGAAT TATGTCCGTG AAAAGACCAG ATGTCCTAAA AGCTAGACTG
    TGGATTGAGT TTGAATCAGA GAAAGGTCTT GACTATGGGG GTGTGGCCAG AGAATGGTTC
    TTCTTACTGT CCAAAGAGAT GTTCAACCCC TACTACGGCC TCTTTGAGTA CTCTGCCACG
    GACAACTACA CCCTTCAGAT CAACCCTAAT TCAGGCCTCT GTAATGAGGA TCATTTGTCC
    TACTTCACTT TTATTGGAAG AGTTGCTGGT CTGGCCGTAT TTCATGGGAA GCTCTTAGAT
    GGTTTCTTCA TTAGACCATT TTACAAGATG ATGTTGGGAA AGCAGATAAC CCTGAATGAC
    ATGGAATCTG TGGATAGTGA ATATTACAAC TCTTTGAAAT GGATCCTGGA GAATGACCCT
    ACTGAGCTGG ACCTCATGTT CTGCATAGAC GAAGAAAACT TTGGACAGAC ATATCAAGTG
    GATTTGAAGC CCAATGGGTC AGAAATAATG GTCACAAATG AAAACAAAAG GGAATATATC
    GACTTAGTCA TCCAGTGGAG ATTTGTGAAC AGGGTCCAGA AGCAGATGAA CGCCTTCTTG
    GAGGGATTCA CAGAACTACT TCCTATTGAT TTGATTAAAA TTTTTGATGA AAATGAGCTG
    GAGTTGCTCA TGTGCGGCCT CGGTGATGTG GATGTGAATG ACTGGAGACA GCATTCTATT
    TACAAGAACG GCTACTGCCC AAACCACCCC GTCATTCAGT GGTTCTGGAA GGCTGTGCTA
    CTCATGGACG CCGAAAAGCG TATCCGGTTA CTGCAGTTTG TCACAGGGAC ATCGCGAGTA
    CCTATGAATG GATTTGCCGA ACTTTATGGT TCCAATGGTC CTCAGCTGTT TACAATAGAG
    CAATGGGGCA GTCCTGAGAA ACTGCCCAGA GCTCACACAT GCTTTAATCG CCTTGACTTA
    CCTCCATATG AAACCTTTGA AGATTTACGA GAGAAACTTC TCATGGCCGT GGAAAATGCT
    CAAGGATTTG AAGGGGTGGA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu14336
    Clone ID Related Accession (Same CDS sequence) NM_001144968.1 , NM_001144968.2
    Accession Version NM_001144968.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2904bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-20
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform 4
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC107896.7, AY112984.2, BC032597.2 and AC090236.4. On May 31, 2019 this sequence version replaced NM_001144968.1. Transcript Variant: This variant (g) uses an alternate first exon, compared to variant j, which results in the use of a different start codon and a protein (isoform 4) with a shorter and distinct N-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript was derived from the reference genome assembly. The genomic coordinates were determined from alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY112984.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAGACGTC TCGCATTTGA GCAGGGAGAG TCCCGTATTC TCAGAGTAAA AGTTGTTTCT 
    GGAATTGATC TCGCCAAAAA GGACATCTTT GGAGCCAGTG ATCCGTATGT GAAACTTTCA
    TTGTACGTAG CGGATGAGAA TAGAGAACTT GCTTTGGTCC AGACAAAAAC AATTAAAAAG
    ACACTGAACC CAAAATGGAA TGAAGAATTT TATTTCAGGG TAAACCCATC TAATCACAGA
    CTCCTATTTG AAGTATTTGA CGAAAATAGA CTGACACGAG ACGACTTCCT GGGCCAGGTG
    GACGTGCCCC TTAGTCACCT TCCGACAGAA GATCCAACCA TGGAGCGACC CTATACATTT
    AAGGACTTTC TCCTCAGACC AAGAAGTCAT AAGTCTCGAG TTAAGGGATT TTTGCGATTG
    AAAATGGCCT ATATGCCAAA AAATGGAGGT CAAGATGAAG AAAACAGTGA CCAGAGGGAT
    GACATGGAGC ATGGATGGGA AGTTGTTGAC TCAAATGACT CGGCTTCTCA GCACCAAGAG
    GAACTTCCTC CTCCTCCTCT GCCTCCCGGG TGGGAAGAAA AAGTGGACAA TTTAGGCCGA
    ACTTACTATG TCAACCACAA CAACCGGACC ACTCAGTGGC ACAGACCAAG CCTGATGGAC
    GTGTCCTCGG AGTCGGACAA TAACATCAGA CAGATCAACC AGGAGGCAGC ACACCGGCGC
    TTCCGCTCCC GCAGGCACAT CAGCGAAGAC TTGGAGCCCG AGCCCTCGGA GGGCGGGGAT
    GTCCCCGAGC CTTGGGAGAC CATTTCAGAG GAAGTGAATA TCGCTGGAGA CTCTCTCGGT
    CTGGCTCTGC CCCCACCACC GGCCTCCCCA GGATCTCGGA CCAGCCCTCA GGAGCTGTCA
    GAGGAACTAA GCAGAAGGCT TCAGATCACT CCAGACTCCA ATGGGGAACA GTTCAGCTCT
    TTGATTCAAA GAGAACCCTC CTCAAGGTTG AGGTCATGCA GTGTCACCGA CGCAGTTGCA
    GAACAGGGCC ATCTACCACC GCCCAGTGCC CCAGCTGGGA GAGCGCGTTC ATCAACTGTC
    ACGGGTGGTG AGGAACCAAC GCCATCAGTG GCCTATGTAC ATACCACGCC GGGTCTGCCT
    TCAGGCTGGG AAGAAAGAAA AGATGCTAAG GGGCGCACAT ACTATGTCAA TCATAACAAT
    CGAACCACAA CTTGGACTCG ACCTATCATG CAGCTTGCAG AAGATGGTGC GTCCGGATCA
    GCCACAAACA GTAACAACCA TCTAATCGAG CCTCAGATCC GCCGGCCTCG TAGCCTCAGC
    TCGCCAACAG TAACTTTATC TGCCCCGCTG GAGGGTGCCA AGGACTCACC CGTACGTCGG
    GCTGTGAAAG ACACCCTTTC CAACCCACAG TCCCCACAGC CATCACCTTA CAACTCCCCC
    AAACCACAAC ACAAAGTCAC ACAGAGCTTC TTGCCACCCG GCTGGGAAAT GAGGATAGCG
    CCAAACGGCC GGCCCTTCTT CATTGATCAT AACACAAAGA CTACAACCTG GGAAGATCCA
    CGTTTGAAAT TTCCAGTACA TATGCGGTCA AAGACATCTT TAAACCCCAA TGACCTTGGC
    CCCCTTCCTC CTGGCTGGGA AGAAAGAATT CACTTGGATG GCCGAACGTT TTATATTGAT
    CATAATAGCA AAATTACTCA GTGGGAAGAC CCAAGACTGC AGAACCCAGC TATTACTGGT
    CCGGCTGTCC CTTACTCCAG AGAATTTAAG CAGAAATATG ACTACTTCAG GAAGAAATTA
    AAGAAACCTG CTGATATCCC CAATAGGTTT GAAATGAAAC TTCACAGAAA TAACATATTT
    GAAGAGTCCT ATCGGAGAAT TATGTCCGTG AAAAGACCAG ATGTCCTAAA AGCTAGACTG
    TGGATTGAGT TTGAATCAGA GAAAGGTCTT GACTATGGGG GTGTGGCCAG AGAATGGTTC
    TTCTTACTGT CCAAAGAGAT GTTCAACCCC TACTACGGCC TCTTTGAGTA CTCTGCCACG
    GACAACTACA CCCTTCAGAT CAACCCTAAT TCAGGCCTCT GTAATGAGGA TCATTTGTCC
    TACTTCACTT TTATTGGAAG AGTTGCTGGT CTGGCCGTAT TTCATGGGAA GCTCTTAGAT
    GGTTTCTTCA TTAGACCATT TTACAAGATG ATGTTGGGAA AGCAGATAAC CCTGAATGAC
    ATGGAATCTG TGGATAGTGA ATATTACAAC TCTTTGAAAT GGATCCTGGA GAATGACCCT
    ACTGAGCTGG ACCTCATGTT CTGCATAGAC GAAGAAAACT TTGGACAGAC ATATCAAGTG
    GATTTGAAGC CCAATGGGTC AGAAATAATG GTCACAAATG AAAACAAAAG GGAATATATC
    GACTTAGTCA TCCAGTGGAG ATTTGTGAAC AGGGTCCAGA AGCAGATGAA CGCCTTCTTG
    GAGGGATTCA CAGAACTACT TCCTATTGAT TTGATTAAAA TTTTTGATGA AAATGAGCTG
    GAGTTGCTCA TGTGCGGCCT CGGTGATGTG GATGTGAATG ACTGGAGACA GCATTCTATT
    TACAAGAACG GCTACTGCCC AAACCACCCC GTCATTCAGT GGTTCTGGAA GGCTGTGCTA
    CTCATGGACG CCGAAAAGCG TATCCGGTTA CTGCAGTTTG TCACAGGGAC ATCGCGAGTA
    CCTATGAATG GATTTGCCGA ACTTTATGGT TCCAATGGTC CTCAGCTGTT TACAATAGAG
    CAATGGGGCA GTCCTGAGAA ACTGCCCAGA GCTCACACAT GCTTTAATCG CCTTGACTTA
    CCTCCATATG AAACCTTTGA AGATTTACGA GAGAAACTTC TCATGGCCGT GGAAAATGCT
    CAAGGATTTG AAGGGGTGGA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001138440.1
    CDS217..3120
    Translation

    Target ORF information:

    RefSeq Version NM_001144968.2
    Organism Homo sapiens(human)
    Definition Homo sapiens NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant g, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001144968.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAGACGTC TCGCATTTGA GCAGGGAGAG TCCCGTATTC TCAGAGTAAA AGTTGTTTCT 
    GGAATTGATC TCGCCAAAAA GGACATCTTT GGAGCCAGTG ATCCGTATGT GAAACTTTCA
    TTGTACGTAG CGGATGAGAA TAGAGAACTT GCTTTGGTCC AGACAAAAAC AATTAAAAAG
    ACACTGAACC CAAAATGGAA TGAAGAATTT TATTTCAGGG TAAACCCATC TAATCACAGA
    CTCCTATTTG AAGTATTTGA CGAAAATAGA CTGACACGAG ACGACTTCCT GGGCCAGGTG
    GACGTGCCCC TTAGTCACCT TCCGACAGAA GATCCAACCA TGGAGCGACC CTATACATTT
    AAGGACTTTC TCCTCAGACC AAGAAGTCAT AAGTCTCGAG TTAAGGGATT TTTGCGATTG
    AAAATGGCCT ATATGCCAAA AAATGGAGGT CAAGATGAAG AAAACAGTGA CCAGAGGGAT
    GACATGGAGC ATGGATGGGA AGTTGTTGAC TCAAATGACT CGGCTTCTCA GCACCAAGAG
    GAACTTCCTC CTCCTCCTCT GCCTCCCGGG TGGGAAGAAA AAGTGGACAA TTTAGGCCGA
    ACTTACTATG TCAACCACAA CAACCGGACC ACTCAGTGGC ACAGACCAAG CCTGATGGAC
    GTGTCCTCGG AGTCGGACAA TAACATCAGA CAGATCAACC AGGAGGCAGC ACACCGGCGC
    TTCCGCTCCC GCAGGCACAT CAGCGAAGAC TTGGAGCCCG AGCCCTCGGA GGGCGGGGAT
    GTCCCCGAGC CTTGGGAGAC CATTTCAGAG GAAGTGAATA TCGCTGGAGA CTCTCTCGGT
    CTGGCTCTGC CCCCACCACC GGCCTCCCCA GGATCTCGGA CCAGCCCTCA GGAGCTGTCA
    GAGGAACTAA GCAGAAGGCT TCAGATCACT CCAGACTCCA ATGGGGAACA GTTCAGCTCT
    TTGATTCAAA GAGAACCCTC CTCAAGGTTG AGGTCATGCA GTGTCACCGA CGCAGTTGCA
    GAACAGGGCC ATCTACCACC GCCCAGTGCC CCAGCTGGGA GAGCGCGTTC ATCAACTGTC
    ACGGGTGGTG AGGAACCAAC GCCATCAGTG GCCTATGTAC ATACCACGCC GGGTCTGCCT
    TCAGGCTGGG AAGAAAGAAA AGATGCTAAG GGGCGCACAT ACTATGTCAA TCATAACAAT
    CGAACCACAA CTTGGACTCG ACCTATCATG CAGCTTGCAG AAGATGGTGC GTCCGGATCA
    GCCACAAACA GTAACAACCA TCTAATCGAG CCTCAGATCC GCCGGCCTCG TAGCCTCAGC
    TCGCCAACAG TAACTTTATC TGCCCCGCTG GAGGGTGCCA AGGACTCACC CGTACGTCGG
    GCTGTGAAAG ACACCCTTTC CAACCCACAG TCCCCACAGC CATCACCTTA CAACTCCCCC
    AAACCACAAC ACAAAGTCAC ACAGAGCTTC TTGCCACCCG GCTGGGAAAT GAGGATAGCG
    CCAAACGGCC GGCCCTTCTT CATTGATCAT AACACAAAGA CTACAACCTG GGAAGATCCA
    CGTTTGAAAT TTCCAGTACA TATGCGGTCA AAGACATCTT TAAACCCCAA TGACCTTGGC
    CCCCTTCCTC CTGGCTGGGA AGAAAGAATT CACTTGGATG GCCGAACGTT TTATATTGAT
    CATAATAGCA AAATTACTCA GTGGGAAGAC CCAAGACTGC AGAACCCAGC TATTACTGGT
    CCGGCTGTCC CTTACTCCAG AGAATTTAAG CAGAAATATG ACTACTTCAG GAAGAAATTA
    AAGAAACCTG CTGATATCCC CAATAGGTTT GAAATGAAAC TTCACAGAAA TAACATATTT
    GAAGAGTCCT ATCGGAGAAT TATGTCCGTG AAAAGACCAG ATGTCCTAAA AGCTAGACTG
    TGGATTGAGT TTGAATCAGA GAAAGGTCTT GACTATGGGG GTGTGGCCAG AGAATGGTTC
    TTCTTACTGT CCAAAGAGAT GTTCAACCCC TACTACGGCC TCTTTGAGTA CTCTGCCACG
    GACAACTACA CCCTTCAGAT CAACCCTAAT TCAGGCCTCT GTAATGAGGA TCATTTGTCC
    TACTTCACTT TTATTGGAAG AGTTGCTGGT CTGGCCGTAT TTCATGGGAA GCTCTTAGAT
    GGTTTCTTCA TTAGACCATT TTACAAGATG ATGTTGGGAA AGCAGATAAC CCTGAATGAC
    ATGGAATCTG TGGATAGTGA ATATTACAAC TCTTTGAAAT GGATCCTGGA GAATGACCCT
    ACTGAGCTGG ACCTCATGTT CTGCATAGAC GAAGAAAACT TTGGACAGAC ATATCAAGTG
    GATTTGAAGC CCAATGGGTC AGAAATAATG GTCACAAATG AAAACAAAAG GGAATATATC
    GACTTAGTCA TCCAGTGGAG ATTTGTGAAC AGGGTCCAGA AGCAGATGAA CGCCTTCTTG
    GAGGGATTCA CAGAACTACT TCCTATTGAT TTGATTAAAA TTTTTGATGA AAATGAGCTG
    GAGTTGCTCA TGTGCGGCCT CGGTGATGTG GATGTGAATG ACTGGAGACA GCATTCTATT
    TACAAGAACG GCTACTGCCC AAACCACCCC GTCATTCAGT GGTTCTGGAA GGCTGTGCTA
    CTCATGGACG CCGAAAAGCG TATCCGGTTA CTGCAGTTTG TCACAGGGAC ATCGCGAGTA
    CCTATGAATG GATTTGCCGA ACTTTATGGT TCCAATGGTC CTCAGCTGTT TACAATAGAG
    CAATGGGGCA GTCCTGAGAA ACTGCCCAGA GCTCACACAT GCTTTAATCG CCTTGACTTA
    CCTCCATATG AAACCTTTGA AGATTTACGA GAGAAACTTC TCATGGCCGT GGAAAATGCT
    CAAGGATTTG AAGGGGTGGA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu14499
    Clone ID Related Accession (Same CDS sequence) NM_001144969.2 , NM_001144969.1
    Accession Version NM_001144969.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2844bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-20
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform 5
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC107896.7, AY112985.2, DQ181796.1, BP260852.1, AF070601.1, DA759940.1, AC090236.4 and DB317092.1. On May 31, 2019 this sequence version replaced NM_001144969.1. Transcript Variant: This variant (h) uses an alternate first exon and lacks a downstream in-frame exon, compared to variant j, which results in the use of a different start codon and a protein (isoform 5) with a shorter and distinct N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY112985.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAGACGTC TCGCATTTGA GCAGGGAGAG TCCCGTATTC TCAGAGTAAA AGTTGTTTCT 
    GGAATTGATC TCGCCAAAAA GGACATCTTT GGAGCCAGTG ATCCGTATGT GAAACTTTCA
    TTGTACGTAG CGGATGAGAA TAGAGAACTT GCTTTGGTCC AGACAAAAAC AATTAAAAAG
    ACACTGAACC CAAAATGGAA TGAAGAATTT TATTTCAGGG TAAACCCATC TAATCACAGA
    CTCCTATTTG AAGTATTTGA CGAAAATAGA CTGACACGAG ACGACTTCCT GGGCCAGGTG
    GACGTGCCCC TTAGTCACCT TCCGACAGAA GATCCAACCA TGGAGCGACC CTATACATTT
    AAGGACTTTC TCCTCAGACC AAGAAGTCAT AAGTCTCGAG TTAAGGGATT TTTGCGATTG
    AAAATGGCCT ATATGCCAAA AAATGGAGGT CAAGATGAAG AAAACAGTGA CCAGAGGGAT
    GACATGGAGC ATGGATGGGA AGTTGTTGAC TCAAATGACT CGGCTTCTCA GCACCAAGAG
    GAACTTCCTC CTCCTCCTCT GCCTCCCGGG TGGGAAGAAA AAGTGGACAA TTTAGGCCGA
    ACTTACTATG TCAACCACAA CAACCGGACC ACTCAGTGGC ACAGACCAAG CCTGATGGAC
    GTGTCCTCGG AGTCGGACAA TAACATCAGA CAGATCAACC AGGAGGCAGC ACACCGGCGC
    TTCCGCTCCC GCAGGCACAT CAGCGAAGAC TTGGAGCCCG AGCCCTCGGA GGGCGGGGAT
    GTCCCCGAGC CTTGGGAGAC CATTTCAGAG GAAGTGAATA TCGCTGGAGA CTCTCTCGGT
    CTGGCTCTGC CCCCACCACC GGCCTCCCCA GGATCTCGGA CCAGCCCTCA GGAGCTGTCA
    GAGGAACTAA GCAGAAGGCT TCAGATCACT CCAGACTCCA ATGGGGAACA GTTCAGCTCT
    TTGATTCAAA GAGAACCCTC CTCAAGGTTG AGGTCATGCA GTGTCACCGA CGCAGTTGCA
    GAACAGGGCC ATCTACCACC GCCATCAGTG GCCTATGTAC ATACCACGCC GGGTCTGCCT
    TCAGGCTGGG AAGAAAGAAA AGATGCTAAG GGGCGCACAT ACTATGTCAA TCATAACAAT
    CGAACCACAA CTTGGACTCG ACCTATCATG CAGCTTGCAG AAGATGGTGC GTCCGGATCA
    GCCACAAACA GTAACAACCA TCTAATCGAG CCTCAGATCC GCCGGCCTCG TAGCCTCAGC
    TCGCCAACAG TAACTTTATC TGCCCCGCTG GAGGGTGCCA AGGACTCACC CGTACGTCGG
    GCTGTGAAAG ACACCCTTTC CAACCCACAG TCCCCACAGC CATCACCTTA CAACTCCCCC
    AAACCACAAC ACAAAGTCAC ACAGAGCTTC TTGCCACCCG GCTGGGAAAT GAGGATAGCG
    CCAAACGGCC GGCCCTTCTT CATTGATCAT AACACAAAGA CTACAACCTG GGAAGATCCA
    CGTTTGAAAT TTCCAGTACA TATGCGGTCA AAGACATCTT TAAACCCCAA TGACCTTGGC
    CCCCTTCCTC CTGGCTGGGA AGAAAGAATT CACTTGGATG GCCGAACGTT TTATATTGAT
    CATAATAGCA AAATTACTCA GTGGGAAGAC CCAAGACTGC AGAACCCAGC TATTACTGGT
    CCGGCTGTCC CTTACTCCAG AGAATTTAAG CAGAAATATG ACTACTTCAG GAAGAAATTA
    AAGAAACCTG CTGATATCCC CAATAGGTTT GAAATGAAAC TTCACAGAAA TAACATATTT
    GAAGAGTCCT ATCGGAGAAT TATGTCCGTG AAAAGACCAG ATGTCCTAAA AGCTAGACTG
    TGGATTGAGT TTGAATCAGA GAAAGGTCTT GACTATGGGG GTGTGGCCAG AGAATGGTTC
    TTCTTACTGT CCAAAGAGAT GTTCAACCCC TACTACGGCC TCTTTGAGTA CTCTGCCACG
    GACAACTACA CCCTTCAGAT CAACCCTAAT TCAGGCCTCT GTAATGAGGA TCATTTGTCC
    TACTTCACTT TTATTGGAAG AGTTGCTGGT CTGGCCGTAT TTCATGGGAA GCTCTTAGAT
    GGTTTCTTCA TTAGACCATT TTACAAGATG ATGTTGGGAA AGCAGATAAC CCTGAATGAC
    ATGGAATCTG TGGATAGTGA ATATTACAAC TCTTTGAAAT GGATCCTGGA GAATGACCCT
    ACTGAGCTGG ACCTCATGTT CTGCATAGAC GAAGAAAACT TTGGACAGAC ATATCAAGTG
    GATTTGAAGC CCAATGGGTC AGAAATAATG GTCACAAATG AAAACAAAAG GGAATATATC
    GACTTAGTCA TCCAGTGGAG ATTTGTGAAC AGGGTCCAGA AGCAGATGAA CGCCTTCTTG
    GAGGGATTCA CAGAACTACT TCCTATTGAT TTGATTAAAA TTTTTGATGA AAATGAGCTG
    GAGTTGCTCA TGTGCGGCCT CGGTGATGTG GATGTGAATG ACTGGAGACA GCATTCTATT
    TACAAGAACG GCTACTGCCC AAACCACCCC GTCATTCAGT GGTTCTGGAA GGCTGTGCTA
    CTCATGGACG CCGAAAAGCG TATCCGGTTA CTGCAGTTTG TCACAGGGAC ATCGCGAGTA
    CCTATGAATG GATTTGCCGA ACTTTATGGT TCCAATGGTC CTCAGCTGTT TACAATAGAG
    CAATGGGGCA GTCCTGAGAA ACTGCCCAGA GCTCACACAT GCTTTAATCG CCTTGACTTA
    CCTCCATATG AAACCTTTGA AGATTTACGA GAGAAACTTC TCATGGCCGT GGAAAATGCT
    CAAGGATTTG AAGGGGTGGA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001138441.1
    CDS217..3060
    Translation

    Target ORF information:

    RefSeq Version NM_001144969.2
    Organism Homo sapiens(human)
    Definition Homo sapiens NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant h, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001144969.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAGACGTC TCGCATTTGA GCAGGGAGAG TCCCGTATTC TCAGAGTAAA AGTTGTTTCT 
    GGAATTGATC TCGCCAAAAA GGACATCTTT GGAGCCAGTG ATCCGTATGT GAAACTTTCA
    TTGTACGTAG CGGATGAGAA TAGAGAACTT GCTTTGGTCC AGACAAAAAC AATTAAAAAG
    ACACTGAACC CAAAATGGAA TGAAGAATTT TATTTCAGGG TAAACCCATC TAATCACAGA
    CTCCTATTTG AAGTATTTGA CGAAAATAGA CTGACACGAG ACGACTTCCT GGGCCAGGTG
    GACGTGCCCC TTAGTCACCT TCCGACAGAA GATCCAACCA TGGAGCGACC CTATACATTT
    AAGGACTTTC TCCTCAGACC AAGAAGTCAT AAGTCTCGAG TTAAGGGATT TTTGCGATTG
    AAAATGGCCT ATATGCCAAA AAATGGAGGT CAAGATGAAG AAAACAGTGA CCAGAGGGAT
    GACATGGAGC ATGGATGGGA AGTTGTTGAC TCAAATGACT CGGCTTCTCA GCACCAAGAG
    GAACTTCCTC CTCCTCCTCT GCCTCCCGGG TGGGAAGAAA AAGTGGACAA TTTAGGCCGA
    ACTTACTATG TCAACCACAA CAACCGGACC ACTCAGTGGC ACAGACCAAG CCTGATGGAC
    GTGTCCTCGG AGTCGGACAA TAACATCAGA CAGATCAACC AGGAGGCAGC ACACCGGCGC
    TTCCGCTCCC GCAGGCACAT CAGCGAAGAC TTGGAGCCCG AGCCCTCGGA GGGCGGGGAT
    GTCCCCGAGC CTTGGGAGAC CATTTCAGAG GAAGTGAATA TCGCTGGAGA CTCTCTCGGT
    CTGGCTCTGC CCCCACCACC GGCCTCCCCA GGATCTCGGA CCAGCCCTCA GGAGCTGTCA
    GAGGAACTAA GCAGAAGGCT TCAGATCACT CCAGACTCCA ATGGGGAACA GTTCAGCTCT
    TTGATTCAAA GAGAACCCTC CTCAAGGTTG AGGTCATGCA GTGTCACCGA CGCAGTTGCA
    GAACAGGGCC ATCTACCACC GCCATCAGTG GCCTATGTAC ATACCACGCC GGGTCTGCCT
    TCAGGCTGGG AAGAAAGAAA AGATGCTAAG GGGCGCACAT ACTATGTCAA TCATAACAAT
    CGAACCACAA CTTGGACTCG ACCTATCATG CAGCTTGCAG AAGATGGTGC GTCCGGATCA
    GCCACAAACA GTAACAACCA TCTAATCGAG CCTCAGATCC GCCGGCCTCG TAGCCTCAGC
    TCGCCAACAG TAACTTTATC TGCCCCGCTG GAGGGTGCCA AGGACTCACC CGTACGTCGG
    GCTGTGAAAG ACACCCTTTC CAACCCACAG TCCCCACAGC CATCACCTTA CAACTCCCCC
    AAACCACAAC ACAAAGTCAC ACAGAGCTTC TTGCCACCCG GCTGGGAAAT GAGGATAGCG
    CCAAACGGCC GGCCCTTCTT CATTGATCAT AACACAAAGA CTACAACCTG GGAAGATCCA
    CGTTTGAAAT TTCCAGTACA TATGCGGTCA AAGACATCTT TAAACCCCAA TGACCTTGGC
    CCCCTTCCTC CTGGCTGGGA AGAAAGAATT CACTTGGATG GCCGAACGTT TTATATTGAT
    CATAATAGCA AAATTACTCA GTGGGAAGAC CCAAGACTGC AGAACCCAGC TATTACTGGT
    CCGGCTGTCC CTTACTCCAG AGAATTTAAG CAGAAATATG ACTACTTCAG GAAGAAATTA
    AAGAAACCTG CTGATATCCC CAATAGGTTT GAAATGAAAC TTCACAGAAA TAACATATTT
    GAAGAGTCCT ATCGGAGAAT TATGTCCGTG AAAAGACCAG ATGTCCTAAA AGCTAGACTG
    TGGATTGAGT TTGAATCAGA GAAAGGTCTT GACTATGGGG GTGTGGCCAG AGAATGGTTC
    TTCTTACTGT CCAAAGAGAT GTTCAACCCC TACTACGGCC TCTTTGAGTA CTCTGCCACG
    GACAACTACA CCCTTCAGAT CAACCCTAAT TCAGGCCTCT GTAATGAGGA TCATTTGTCC
    TACTTCACTT TTATTGGAAG AGTTGCTGGT CTGGCCGTAT TTCATGGGAA GCTCTTAGAT
    GGTTTCTTCA TTAGACCATT TTACAAGATG ATGTTGGGAA AGCAGATAAC CCTGAATGAC
    ATGGAATCTG TGGATAGTGA ATATTACAAC TCTTTGAAAT GGATCCTGGA GAATGACCCT
    ACTGAGCTGG ACCTCATGTT CTGCATAGAC GAAGAAAACT TTGGACAGAC ATATCAAGTG
    GATTTGAAGC CCAATGGGTC AGAAATAATG GTCACAAATG AAAACAAAAG GGAATATATC
    GACTTAGTCA TCCAGTGGAG ATTTGTGAAC AGGGTCCAGA AGCAGATGAA CGCCTTCTTG
    GAGGGATTCA CAGAACTACT TCCTATTGAT TTGATTAAAA TTTTTGATGA AAATGAGCTG
    GAGTTGCTCA TGTGCGGCCT CGGTGATGTG GATGTGAATG ACTGGAGACA GCATTCTATT
    TACAAGAACG GCTACTGCCC AAACCACCCC GTCATTCAGT GGTTCTGGAA GGCTGTGCTA
    CTCATGGACG CCGAAAAGCG TATCCGGTTA CTGCAGTTTG TCACAGGGAC ATCGCGAGTA
    CCTATGAATG GATTTGCCGA ACTTTATGGT TCCAATGGTC CTCAGCTGTT TACAATAGAG
    CAATGGGGCA GTCCTGAGAA ACTGCCCAGA GCTCACACAT GCTTTAATCG CCTTGACTTA
    CCTCCATATG AAACCTTTGA AGATTTACGA GAGAAACTTC TCATGGCCGT GGAAAATGCT
    CAAGGATTTG AAGGGGTGGA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu14499
    Clone ID Related Accession (Same CDS sequence) NM_001144969.2 , NM_001144969.1
    Accession Version NM_001144969.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2844bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-03
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform 5
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY112985.2, DQ181796.1, BP260852.1, AF070601.1, DA759940.1, AC090236.4 and DB317092.1. Transcript Variant: This variant (h) uses an alternate first exon and lacks a downstream in-frame exon, compared to variant j, which results in the use of a different start codon and a protein (isoform 5) with a shorter and distinct N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY112985.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAGACGTC TCGCATTTGA GCAGGGAGAG TCCCGTATTC TCAGAGTAAA AGTTGTTTCT 
    GGAATTGATC TCGCCAAAAA GGACATCTTT GGAGCCAGTG ATCCGTATGT GAAACTTTCA
    TTGTACGTAG CGGATGAGAA TAGAGAACTT GCTTTGGTCC AGACAAAAAC AATTAAAAAG
    ACACTGAACC CAAAATGGAA TGAAGAATTT TATTTCAGGG TAAACCCATC TAATCACAGA
    CTCCTATTTG AAGTATTTGA CGAAAATAGA CTGACACGAG ACGACTTCCT GGGCCAGGTG
    GACGTGCCCC TTAGTCACCT TCCGACAGAA GATCCAACCA TGGAGCGACC CTATACATTT
    AAGGACTTTC TCCTCAGACC AAGAAGTCAT AAGTCTCGAG TTAAGGGATT TTTGCGATTG
    AAAATGGCCT ATATGCCAAA AAATGGAGGT CAAGATGAAG AAAACAGTGA CCAGAGGGAT
    GACATGGAGC ATGGATGGGA AGTTGTTGAC TCAAATGACT CGGCTTCTCA GCACCAAGAG
    GAACTTCCTC CTCCTCCTCT GCCTCCCGGG TGGGAAGAAA AAGTGGACAA TTTAGGCCGA
    ACTTACTATG TCAACCACAA CAACCGGACC ACTCAGTGGC ACAGACCAAG CCTGATGGAC
    GTGTCCTCGG AGTCGGACAA TAACATCAGA CAGATCAACC AGGAGGCAGC ACACCGGCGC
    TTCCGCTCCC GCAGGCACAT CAGCGAAGAC TTGGAGCCCG AGCCCTCGGA GGGCGGGGAT
    GTCCCCGAGC CTTGGGAGAC CATTTCAGAG GAAGTGAATA TCGCTGGAGA CTCTCTCGGT
    CTGGCTCTGC CCCCACCACC GGCCTCCCCA GGATCTCGGA CCAGCCCTCA GGAGCTGTCA
    GAGGAACTAA GCAGAAGGCT TCAGATCACT CCAGACTCCA ATGGGGAACA GTTCAGCTCT
    TTGATTCAAA GAGAACCCTC CTCAAGGTTG AGGTCATGCA GTGTCACCGA CGCAGTTGCA
    GAACAGGGCC ATCTACCACC GCCATCAGTG GCCTATGTAC ATACCACGCC GGGTCTGCCT
    TCAGGCTGGG AAGAAAGAAA AGATGCTAAG GGGCGCACAT ACTATGTCAA TCATAACAAT
    CGAACCACAA CTTGGACTCG ACCTATCATG CAGCTTGCAG AAGATGGTGC GTCCGGATCA
    GCCACAAACA GTAACAACCA TCTAATCGAG CCTCAGATCC GCCGGCCTCG TAGCCTCAGC
    TCGCCAACAG TAACTTTATC TGCCCCGCTG GAGGGTGCCA AGGACTCACC CGTACGTCGG
    GCTGTGAAAG ACACCCTTTC CAACCCACAG TCCCCACAGC CATCACCTTA CAACTCCCCC
    AAACCACAAC ACAAAGTCAC ACAGAGCTTC TTGCCACCCG GCTGGGAAAT GAGGATAGCG
    CCAAACGGCC GGCCCTTCTT CATTGATCAT AACACAAAGA CTACAACCTG GGAAGATCCA
    CGTTTGAAAT TTCCAGTACA TATGCGGTCA AAGACATCTT TAAACCCCAA TGACCTTGGC
    CCCCTTCCTC CTGGCTGGGA AGAAAGAATT CACTTGGATG GCCGAACGTT TTATATTGAT
    CATAATAGCA AAATTACTCA GTGGGAAGAC CCAAGACTGC AGAACCCAGC TATTACTGGT
    CCGGCTGTCC CTTACTCCAG AGAATTTAAG CAGAAATATG ACTACTTCAG GAAGAAATTA
    AAGAAACCTG CTGATATCCC CAATAGGTTT GAAATGAAAC TTCACAGAAA TAACATATTT
    GAAGAGTCCT ATCGGAGAAT TATGTCCGTG AAAAGACCAG ATGTCCTAAA AGCTAGACTG
    TGGATTGAGT TTGAATCAGA GAAAGGTCTT GACTATGGGG GTGTGGCCAG AGAATGGTTC
    TTCTTACTGT CCAAAGAGAT GTTCAACCCC TACTACGGCC TCTTTGAGTA CTCTGCCACG
    GACAACTACA CCCTTCAGAT CAACCCTAAT TCAGGCCTCT GTAATGAGGA TCATTTGTCC
    TACTTCACTT TTATTGGAAG AGTTGCTGGT CTGGCCGTAT TTCATGGGAA GCTCTTAGAT
    GGTTTCTTCA TTAGACCATT TTACAAGATG ATGTTGGGAA AGCAGATAAC CCTGAATGAC
    ATGGAATCTG TGGATAGTGA ATATTACAAC TCTTTGAAAT GGATCCTGGA GAATGACCCT
    ACTGAGCTGG ACCTCATGTT CTGCATAGAC GAAGAAAACT TTGGACAGAC ATATCAAGTG
    GATTTGAAGC CCAATGGGTC AGAAATAATG GTCACAAATG AAAACAAAAG GGAATATATC
    GACTTAGTCA TCCAGTGGAG ATTTGTGAAC AGGGTCCAGA AGCAGATGAA CGCCTTCTTG
    GAGGGATTCA CAGAACTACT TCCTATTGAT TTGATTAAAA TTTTTGATGA AAATGAGCTG
    GAGTTGCTCA TGTGCGGCCT CGGTGATGTG GATGTGAATG ACTGGAGACA GCATTCTATT
    TACAAGAACG GCTACTGCCC AAACCACCCC GTCATTCAGT GGTTCTGGAA GGCTGTGCTA
    CTCATGGACG CCGAAAAGCG TATCCGGTTA CTGCAGTTTG TCACAGGGAC ATCGCGAGTA
    CCTATGAATG GATTTGCCGA ACTTTATGGT TCCAATGGTC CTCAGCTGTT TACAATAGAG
    CAATGGGGCA GTCCTGAGAA ACTGCCCAGA GCTCACACAT GCTTTAATCG CCTTGACTTA
    CCTCCATATG AAACCTTTGA AGATTTACGA GAGAAACTTC TCATGGCCGT GGAAAATGCT
    CAAGGATTTG AAGGGGTGGA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001138441.1
    CDS204..3047
    Translation

    Target ORF information:

    RefSeq Version NM_001144969.1
    Organism Homo sapiens(human)
    Definition Homo sapiens neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant h, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001144969.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAGACGTC TCGCATTTGA GCAGGGAGAG TCCCGTATTC TCAGAGTAAA AGTTGTTTCT 
    GGAATTGATC TCGCCAAAAA GGACATCTTT GGAGCCAGTG ATCCGTATGT GAAACTTTCA
    TTGTACGTAG CGGATGAGAA TAGAGAACTT GCTTTGGTCC AGACAAAAAC AATTAAAAAG
    ACACTGAACC CAAAATGGAA TGAAGAATTT TATTTCAGGG TAAACCCATC TAATCACAGA
    CTCCTATTTG AAGTATTTGA CGAAAATAGA CTGACACGAG ACGACTTCCT GGGCCAGGTG
    GACGTGCCCC TTAGTCACCT TCCGACAGAA GATCCAACCA TGGAGCGACC CTATACATTT
    AAGGACTTTC TCCTCAGACC AAGAAGTCAT AAGTCTCGAG TTAAGGGATT TTTGCGATTG
    AAAATGGCCT ATATGCCAAA AAATGGAGGT CAAGATGAAG AAAACAGTGA CCAGAGGGAT
    GACATGGAGC ATGGATGGGA AGTTGTTGAC TCAAATGACT CGGCTTCTCA GCACCAAGAG
    GAACTTCCTC CTCCTCCTCT GCCTCCCGGG TGGGAAGAAA AAGTGGACAA TTTAGGCCGA
    ACTTACTATG TCAACCACAA CAACCGGACC ACTCAGTGGC ACAGACCAAG CCTGATGGAC
    GTGTCCTCGG AGTCGGACAA TAACATCAGA CAGATCAACC AGGAGGCAGC ACACCGGCGC
    TTCCGCTCCC GCAGGCACAT CAGCGAAGAC TTGGAGCCCG AGCCCTCGGA GGGCGGGGAT
    GTCCCCGAGC CTTGGGAGAC CATTTCAGAG GAAGTGAATA TCGCTGGAGA CTCTCTCGGT
    CTGGCTCTGC CCCCACCACC GGCCTCCCCA GGATCTCGGA CCAGCCCTCA GGAGCTGTCA
    GAGGAACTAA GCAGAAGGCT TCAGATCACT CCAGACTCCA ATGGGGAACA GTTCAGCTCT
    TTGATTCAAA GAGAACCCTC CTCAAGGTTG AGGTCATGCA GTGTCACCGA CGCAGTTGCA
    GAACAGGGCC ATCTACCACC GCCATCAGTG GCCTATGTAC ATACCACGCC GGGTCTGCCT
    TCAGGCTGGG AAGAAAGAAA AGATGCTAAG GGGCGCACAT ACTATGTCAA TCATAACAAT
    CGAACCACAA CTTGGACTCG ACCTATCATG CAGCTTGCAG AAGATGGTGC GTCCGGATCA
    GCCACAAACA GTAACAACCA TCTAATCGAG CCTCAGATCC GCCGGCCTCG TAGCCTCAGC
    TCGCCAACAG TAACTTTATC TGCCCCGCTG GAGGGTGCCA AGGACTCACC CGTACGTCGG
    GCTGTGAAAG ACACCCTTTC CAACCCACAG TCCCCACAGC CATCACCTTA CAACTCCCCC
    AAACCACAAC ACAAAGTCAC ACAGAGCTTC TTGCCACCCG GCTGGGAAAT GAGGATAGCG
    CCAAACGGCC GGCCCTTCTT CATTGATCAT AACACAAAGA CTACAACCTG GGAAGATCCA
    CGTTTGAAAT TTCCAGTACA TATGCGGTCA AAGACATCTT TAAACCCCAA TGACCTTGGC
    CCCCTTCCTC CTGGCTGGGA AGAAAGAATT CACTTGGATG GCCGAACGTT TTATATTGAT
    CATAATAGCA AAATTACTCA GTGGGAAGAC CCAAGACTGC AGAACCCAGC TATTACTGGT
    CCGGCTGTCC CTTACTCCAG AGAATTTAAG CAGAAATATG ACTACTTCAG GAAGAAATTA
    AAGAAACCTG CTGATATCCC CAATAGGTTT GAAATGAAAC TTCACAGAAA TAACATATTT
    GAAGAGTCCT ATCGGAGAAT TATGTCCGTG AAAAGACCAG ATGTCCTAAA AGCTAGACTG
    TGGATTGAGT TTGAATCAGA GAAAGGTCTT GACTATGGGG GTGTGGCCAG AGAATGGTTC
    TTCTTACTGT CCAAAGAGAT GTTCAACCCC TACTACGGCC TCTTTGAGTA CTCTGCCACG
    GACAACTACA CCCTTCAGAT CAACCCTAAT TCAGGCCTCT GTAATGAGGA TCATTTGTCC
    TACTTCACTT TTATTGGAAG AGTTGCTGGT CTGGCCGTAT TTCATGGGAA GCTCTTAGAT
    GGTTTCTTCA TTAGACCATT TTACAAGATG ATGTTGGGAA AGCAGATAAC CCTGAATGAC
    ATGGAATCTG TGGATAGTGA ATATTACAAC TCTTTGAAAT GGATCCTGGA GAATGACCCT
    ACTGAGCTGG ACCTCATGTT CTGCATAGAC GAAGAAAACT TTGGACAGAC ATATCAAGTG
    GATTTGAAGC CCAATGGGTC AGAAATAATG GTCACAAATG AAAACAAAAG GGAATATATC
    GACTTAGTCA TCCAGTGGAG ATTTGTGAAC AGGGTCCAGA AGCAGATGAA CGCCTTCTTG
    GAGGGATTCA CAGAACTACT TCCTATTGAT TTGATTAAAA TTTTTGATGA AAATGAGCTG
    GAGTTGCTCA TGTGCGGCCT CGGTGATGTG GATGTGAATG ACTGGAGACA GCATTCTATT
    TACAAGAACG GCTACTGCCC AAACCACCCC GTCATTCAGT GGTTCTGGAA GGCTGTGCTA
    CTCATGGACG CCGAAAAGCG TATCCGGTTA CTGCAGTTTG TCACAGGGAC ATCGCGAGTA
    CCTATGAATG GATTTGCCGA ACTTTATGGT TCCAATGGTC CTCAGCTGTT TACAATAGAG
    CAATGGGGCA GTCCTGAGAA ACTGCCCAGA GCTCACACAT GCTTTAATCG CCTTGACTTA
    CCTCCATATG AAACCTTTGA AGATTTACGA GAGAAACTTC TCATGGCCGT GGAAAATGCT
    CAAGGATTTG AAGGGGTGGA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu14655
    Clone ID Related Accession (Same CDS sequence) NM_001243960.1 , NM_001243960.2
    Accession Version NM_001243960.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2736bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-03
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform 7
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC107896.7, BC032597.2 and AC090236.4. Transcript Variant: This variant (i) lacks two consecutive exons in the coding region, but maintains the reading frame, compared to variant j. The encoded isoform (7) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC032597.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144333, SAMEA2149004 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCGACCG GGCTCGGGGA GCCGGTCTAT GGACTTTCCG AAGACGAGGG AGAGTCCCGT 
    ATTCTCAGAG TAAAAGTTGT TTCTGGAATT GATCTCGCCA AAAAGGACAT CTTTGGAGCC
    AGTGATCCGT ATGTGAAACT TTCATTGTAC GTAGCGGATG AGAATAGAGA ACTTGCTTTG
    GTCCAGACAA AAACAATTAA AAAGACACTG AACCCAAAAT GGAATGAAGA ATTTTATTTC
    AGGGTAAACC CATCTAATCA CAGACTCCTA TTTGAAGTAT TTGACGAAAA TAGACTGACA
    CGAGACGACT TCCTGGGCCA GGTGGACGTG CCCCTTAGTC ACCTTCCGAC AGAAGATCCA
    ACCATGGAGC GACCCTATAC ATTTAAGGAC TTTCTCCTCA GACCAAGAAG TCATAAGTCT
    CGAGTTAAGG GATTTTTGCG ATTGAAAATG GCCTATATGC CAAAAAATGG AGGTCAAGAT
    GAAGAAAACA GTGACCAGAG GGATGACATG GAGCATGGAT GGGAAGTTGT TGACTCAAAT
    GACTCGGCTT CTCAGCACCA AGAGGAACTT CCTCCTCCTC CTCTGCCTCC CGGGTGGGAA
    GAAAAAGTGG ACAATTTAGG CCGAACTTAC TATGTCAACC ACAACAACCG GACCACTCAG
    TGGCACAGAC CAAGCCTGAT GGACGTGTCC TCGGAGTCGG ACAATAACAT CAGACAGATC
    AACCAGGAGG CAGCACACCG GCGCTTCCGC TCCCGCAGGC ACATCAGCGA AGACTTGGAG
    CCCGAGCCCT CGGAGGGCGG GGATGTCCCC GAGCCTTGGG AGACCATTTC AGAGGAAGTG
    AATATCGCTG GAGACTCTCT CGGTCTGGCT CTGCCCCCAC CACCGGCCTC CCCAGGATCT
    CGGACCAGCC CTCAGGAGCT GTCAGAGGAA CTAAGCAGAA GGCTTCAGAT CACTCCAGAC
    TCCAATGGGG AACAGTTCAG CTCTTTGATT CAAAGAGAAC CCTCCTCAAG GTTGAGGTCA
    TGCAGTGTCA CCGACGCAGT TGCAGAACAG GGCCATCTAC CACCGCTTGC AGAAGATGGT
    GCGTCCGGAT CAGCCACAAA CAGTAACAAC CATCTAATCG AGCCTCAGAT CCGCCGGCCT
    CGTAGCCTCA GCTCGCCAAC AGTAACTTTA TCTGCCCCGC TGGAGGGTGC CAAGGACTCA
    CCCGTACGTC GGGCTGTGAA AGACACCCTT TCCAACCCAC AGTCCCCACA GCCATCACCT
    TACAACTCCC CCAAACCACA ACACAAAGTC ACACAGAGCT TCTTGCCACC CGGCTGGGAA
    ATGAGGATAG CGCCAAACGG CCGGCCCTTC TTCATTGATC ATAACACAAA GACTACAACC
    TGGGAAGATC CACGTTTGAA ATTTCCAGTA CATATGCGGT CAAAGACATC TTTAAACCCC
    AATGACCTTG GCCCCCTTCC TCCTGGCTGG GAAGAAAGAA TTCACTTGGA TGGCCGAACG
    TTTTATATTG ATCATAATAG CAAAATTACT CAGTGGGAAG ACCCAAGACT GCAGAACCCA
    GCTATTACTG GTCCGGCTGT CCCTTACTCC AGAGAATTTA AGCAGAAATA TGACTACTTC
    AGGAAGAAAT TAAAGAAACC TGCTGATATC CCCAATAGGT TTGAAATGAA ACTTCACAGA
    AATAACATAT TTGAAGAGTC CTATCGGAGA ATTATGTCCG TGAAAAGACC AGATGTCCTA
    AAAGCTAGAC TGTGGATTGA GTTTGAATCA GAGAAAGGTC TTGACTATGG GGGTGTGGCC
    AGAGAATGGT TCTTCTTACT GTCCAAAGAG ATGTTCAACC CCTACTACGG CCTCTTTGAG
    TACTCTGCCA CGGACAACTA CACCCTTCAG ATCAACCCTA ATTCAGGCCT CTGTAATGAG
    GATCATTTGT CCTACTTCAC TTTTATTGGA AGAGTTGCTG GTCTGGCCGT ATTTCATGGG
    AAGCTCTTAG ATGGTTTCTT CATTAGACCA TTTTACAAGA TGATGTTGGG AAAGCAGATA
    ACCCTGAATG ACATGGAATC TGTGGATAGT GAATATTACA ACTCTTTGAA ATGGATCCTG
    GAGAATGACC CTACTGAGCT GGACCTCATG TTCTGCATAG ACGAAGAAAA CTTTGGACAG
    ACATATCAAG TGGATTTGAA GCCCAATGGG TCAGAAATAA TGGTCACAAA TGAAAACAAA
    AGGGAATATA TCGACTTAGT CATCCAGTGG AGATTTGTGA ACAGGGTCCA GAAGCAGATG
    AACGCCTTCT TGGAGGGATT CACAGAACTA CTTCCTATTG ATTTGATTAA AATTTTTGAT
    GAAAATGAGC TGGAGTTGCT CATGTGCGGC CTCGGTGATG TGGATGTGAA TGACTGGAGA
    CAGCATTCTA TTTACAAGAA CGGCTACTGC CCAAACCACC CCGTCATTCA GTGGTTCTGG
    AAGGCTGTGC TACTCATGGA CGCCGAAAAG CGTATCCGGT TACTGCAGTT TGTCACAGGG
    ACATCGCGAG TACCTATGAA TGGATTTGCC GAACTTTATG GTTCCAATGG TCCTCAGCTG
    TTTACAATAG AGCAATGGGG CAGTCCTGAG AAACTGCCCA GAGCTCACAC ATGCTTTAAT
    CGCCTTGACT TACCTCCATA TGAAACCTTT GAAGATTTAC GAGAGAAACT TCTCATGGCC
    GTGGAAAATG CTCAAGGATT TGAAGGGGTG GATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001230889.1
    CDS284..3019
    Translation

    Target ORF information:

    RefSeq Version NM_001243960.1
    Organism Homo sapiens(human)
    Definition Homo sapiens neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant i, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001243960.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCGACCG GGCTCGGGGA GCCGGTCTAT GGACTTTCCG AAGACGAGGG AGAGTCCCGT 
    ATTCTCAGAG TAAAAGTTGT TTCTGGAATT GATCTCGCCA AAAAGGACAT CTTTGGAGCC
    AGTGATCCGT ATGTGAAACT TTCATTGTAC GTAGCGGATG AGAATAGAGA ACTTGCTTTG
    GTCCAGACAA AAACAATTAA AAAGACACTG AACCCAAAAT GGAATGAAGA ATTTTATTTC
    AGGGTAAACC CATCTAATCA CAGACTCCTA TTTGAAGTAT TTGACGAAAA TAGACTGACA
    CGAGACGACT TCCTGGGCCA GGTGGACGTG CCCCTTAGTC ACCTTCCGAC AGAAGATCCA
    ACCATGGAGC GACCCTATAC ATTTAAGGAC TTTCTCCTCA GACCAAGAAG TCATAAGTCT
    CGAGTTAAGG GATTTTTGCG ATTGAAAATG GCCTATATGC CAAAAAATGG AGGTCAAGAT
    GAAGAAAACA GTGACCAGAG GGATGACATG GAGCATGGAT GGGAAGTTGT TGACTCAAAT
    GACTCGGCTT CTCAGCACCA AGAGGAACTT CCTCCTCCTC CTCTGCCTCC CGGGTGGGAA
    GAAAAAGTGG ACAATTTAGG CCGAACTTAC TATGTCAACC ACAACAACCG GACCACTCAG
    TGGCACAGAC CAAGCCTGAT GGACGTGTCC TCGGAGTCGG ACAATAACAT CAGACAGATC
    AACCAGGAGG CAGCACACCG GCGCTTCCGC TCCCGCAGGC ACATCAGCGA AGACTTGGAG
    CCCGAGCCCT CGGAGGGCGG GGATGTCCCC GAGCCTTGGG AGACCATTTC AGAGGAAGTG
    AATATCGCTG GAGACTCTCT CGGTCTGGCT CTGCCCCCAC CACCGGCCTC CCCAGGATCT
    CGGACCAGCC CTCAGGAGCT GTCAGAGGAA CTAAGCAGAA GGCTTCAGAT CACTCCAGAC
    TCCAATGGGG AACAGTTCAG CTCTTTGATT CAAAGAGAAC CCTCCTCAAG GTTGAGGTCA
    TGCAGTGTCA CCGACGCAGT TGCAGAACAG GGCCATCTAC CACCGCTTGC AGAAGATGGT
    GCGTCCGGAT CAGCCACAAA CAGTAACAAC CATCTAATCG AGCCTCAGAT CCGCCGGCCT
    CGTAGCCTCA GCTCGCCAAC AGTAACTTTA TCTGCCCCGC TGGAGGGTGC CAAGGACTCA
    CCCGTACGTC GGGCTGTGAA AGACACCCTT TCCAACCCAC AGTCCCCACA GCCATCACCT
    TACAACTCCC CCAAACCACA ACACAAAGTC ACACAGAGCT TCTTGCCACC CGGCTGGGAA
    ATGAGGATAG CGCCAAACGG CCGGCCCTTC TTCATTGATC ATAACACAAA GACTACAACC
    TGGGAAGATC CACGTTTGAA ATTTCCAGTA CATATGCGGT CAAAGACATC TTTAAACCCC
    AATGACCTTG GCCCCCTTCC TCCTGGCTGG GAAGAAAGAA TTCACTTGGA TGGCCGAACG
    TTTTATATTG ATCATAATAG CAAAATTACT CAGTGGGAAG ACCCAAGACT GCAGAACCCA
    GCTATTACTG GTCCGGCTGT CCCTTACTCC AGAGAATTTA AGCAGAAATA TGACTACTTC
    AGGAAGAAAT TAAAGAAACC TGCTGATATC CCCAATAGGT TTGAAATGAA ACTTCACAGA
    AATAACATAT TTGAAGAGTC CTATCGGAGA ATTATGTCCG TGAAAAGACC AGATGTCCTA
    AAAGCTAGAC TGTGGATTGA GTTTGAATCA GAGAAAGGTC TTGACTATGG GGGTGTGGCC
    AGAGAATGGT TCTTCTTACT GTCCAAAGAG ATGTTCAACC CCTACTACGG CCTCTTTGAG
    TACTCTGCCA CGGACAACTA CACCCTTCAG ATCAACCCTA ATTCAGGCCT CTGTAATGAG
    GATCATTTGT CCTACTTCAC TTTTATTGGA AGAGTTGCTG GTCTGGCCGT ATTTCATGGG
    AAGCTCTTAG ATGGTTTCTT CATTAGACCA TTTTACAAGA TGATGTTGGG AAAGCAGATA
    ACCCTGAATG ACATGGAATC TGTGGATAGT GAATATTACA ACTCTTTGAA ATGGATCCTG
    GAGAATGACC CTACTGAGCT GGACCTCATG TTCTGCATAG ACGAAGAAAA CTTTGGACAG
    ACATATCAAG TGGATTTGAA GCCCAATGGG TCAGAAATAA TGGTCACAAA TGAAAACAAA
    AGGGAATATA TCGACTTAGT CATCCAGTGG AGATTTGTGA ACAGGGTCCA GAAGCAGATG
    AACGCCTTCT TGGAGGGATT CACAGAACTA CTTCCTATTG ATTTGATTAA AATTTTTGAT
    GAAAATGAGC TGGAGTTGCT CATGTGCGGC CTCGGTGATG TGGATGTGAA TGACTGGAGA
    CAGCATTCTA TTTACAAGAA CGGCTACTGC CCAAACCACC CCGTCATTCA GTGGTTCTGG
    AAGGCTGTGC TACTCATGGA CGCCGAAAAG CGTATCCGGT TACTGCAGTT TGTCACAGGG
    ACATCGCGAG TACCTATGAA TGGATTTGCC GAACTTTATG GTTCCAATGG TCCTCAGCTG
    TTTACAATAG AGCAATGGGG CAGTCCTGAG AAACTGCCCA GAGCTCACAC ATGCTTTAAT
    CGCCTTGACT TACCTCCATA TGAAACCTTT GAAGATTTAC GAGAGAAACT TCTCATGGCC
    GTGGAAAATG CTCAAGGATT TGAAGGGGTG GATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu14655
    Clone ID Related Accession (Same CDS sequence) NM_001243960.1 , NM_001243960.2
    Accession Version NM_001243960.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2736bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-20
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform 7
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC107896.7, BC032597.2 and AC090236.4. On May 31, 2019 this sequence version replaced NM_001243960.1. Transcript Variant: This variant (i) lacks two consecutive exons in the coding region, but maintains the reading frame, compared to variant j. The encoded isoform (7) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC032597.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144333, SAMEA2149004 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCGACCG GGCTCGGGGA GCCGGTCTAT GGACTTTCCG AAGACGAGGG AGAGTCCCGT 
    ATTCTCAGAG TAAAAGTTGT TTCTGGAATT GATCTCGCCA AAAAGGACAT CTTTGGAGCC
    AGTGATCCGT ATGTGAAACT TTCATTGTAC GTAGCGGATG AGAATAGAGA ACTTGCTTTG
    GTCCAGACAA AAACAATTAA AAAGACACTG AACCCAAAAT GGAATGAAGA ATTTTATTTC
    AGGGTAAACC CATCTAATCA CAGACTCCTA TTTGAAGTAT TTGACGAAAA TAGACTGACA
    CGAGACGACT TCCTGGGCCA GGTGGACGTG CCCCTTAGTC ACCTTCCGAC AGAAGATCCA
    ACCATGGAGC GACCCTATAC ATTTAAGGAC TTTCTCCTCA GACCAAGAAG TCATAAGTCT
    CGAGTTAAGG GATTTTTGCG ATTGAAAATG GCCTATATGC CAAAAAATGG AGGTCAAGAT
    GAAGAAAACA GTGACCAGAG GGATGACATG GAGCATGGAT GGGAAGTTGT TGACTCAAAT
    GACTCGGCTT CTCAGCACCA AGAGGAACTT CCTCCTCCTC CTCTGCCTCC CGGGTGGGAA
    GAAAAAGTGG ACAATTTAGG CCGAACTTAC TATGTCAACC ACAACAACCG GACCACTCAG
    TGGCACAGAC CAAGCCTGAT GGACGTGTCC TCGGAGTCGG ACAATAACAT CAGACAGATC
    AACCAGGAGG CAGCACACCG GCGCTTCCGC TCCCGCAGGC ACATCAGCGA AGACTTGGAG
    CCCGAGCCCT CGGAGGGCGG GGATGTCCCC GAGCCTTGGG AGACCATTTC AGAGGAAGTG
    AATATCGCTG GAGACTCTCT CGGTCTGGCT CTGCCCCCAC CACCGGCCTC CCCAGGATCT
    CGGACCAGCC CTCAGGAGCT GTCAGAGGAA CTAAGCAGAA GGCTTCAGAT CACTCCAGAC
    TCCAATGGGG AACAGTTCAG CTCTTTGATT CAAAGAGAAC CCTCCTCAAG GTTGAGGTCA
    TGCAGTGTCA CCGACGCAGT TGCAGAACAG GGCCATCTAC CACCGCTTGC AGAAGATGGT
    GCGTCCGGAT CAGCCACAAA CAGTAACAAC CATCTAATCG AGCCTCAGAT CCGCCGGCCT
    CGTAGCCTCA GCTCGCCAAC AGTAACTTTA TCTGCCCCGC TGGAGGGTGC CAAGGACTCA
    CCCGTACGTC GGGCTGTGAA AGACACCCTT TCCAACCCAC AGTCCCCACA GCCATCACCT
    TACAACTCCC CCAAACCACA ACACAAAGTC ACACAGAGCT TCTTGCCACC CGGCTGGGAA
    ATGAGGATAG CGCCAAACGG CCGGCCCTTC TTCATTGATC ATAACACAAA GACTACAACC
    TGGGAAGATC CACGTTTGAA ATTTCCAGTA CATATGCGGT CAAAGACATC TTTAAACCCC
    AATGACCTTG GCCCCCTTCC TCCTGGCTGG GAAGAAAGAA TTCACTTGGA TGGCCGAACG
    TTTTATATTG ATCATAATAG CAAAATTACT CAGTGGGAAG ACCCAAGACT GCAGAACCCA
    GCTATTACTG GTCCGGCTGT CCCTTACTCC AGAGAATTTA AGCAGAAATA TGACTACTTC
    AGGAAGAAAT TAAAGAAACC TGCTGATATC CCCAATAGGT TTGAAATGAA ACTTCACAGA
    AATAACATAT TTGAAGAGTC CTATCGGAGA ATTATGTCCG TGAAAAGACC AGATGTCCTA
    AAAGCTAGAC TGTGGATTGA GTTTGAATCA GAGAAAGGTC TTGACTATGG GGGTGTGGCC
    AGAGAATGGT TCTTCTTACT GTCCAAAGAG ATGTTCAACC CCTACTACGG CCTCTTTGAG
    TACTCTGCCA CGGACAACTA CACCCTTCAG ATCAACCCTA ATTCAGGCCT CTGTAATGAG
    GATCATTTGT CCTACTTCAC TTTTATTGGA AGAGTTGCTG GTCTGGCCGT ATTTCATGGG
    AAGCTCTTAG ATGGTTTCTT CATTAGACCA TTTTACAAGA TGATGTTGGG AAAGCAGATA
    ACCCTGAATG ACATGGAATC TGTGGATAGT GAATATTACA ACTCTTTGAA ATGGATCCTG
    GAGAATGACC CTACTGAGCT GGACCTCATG TTCTGCATAG ACGAAGAAAA CTTTGGACAG
    ACATATCAAG TGGATTTGAA GCCCAATGGG TCAGAAATAA TGGTCACAAA TGAAAACAAA
    AGGGAATATA TCGACTTAGT CATCCAGTGG AGATTTGTGA ACAGGGTCCA GAAGCAGATG
    AACGCCTTCT TGGAGGGATT CACAGAACTA CTTCCTATTG ATTTGATTAA AATTTTTGAT
    GAAAATGAGC TGGAGTTGCT CATGTGCGGC CTCGGTGATG TGGATGTGAA TGACTGGAGA
    CAGCATTCTA TTTACAAGAA CGGCTACTGC CCAAACCACC CCGTCATTCA GTGGTTCTGG
    AAGGCTGTGC TACTCATGGA CGCCGAAAAG CGTATCCGGT TACTGCAGTT TGTCACAGGG
    ACATCGCGAG TACCTATGAA TGGATTTGCC GAACTTTATG GTTCCAATGG TCCTCAGCTG
    TTTACAATAG AGCAATGGGG CAGTCCTGAG AAACTGCCCA GAGCTCACAC ATGCTTTAAT
    CGCCTTGACT TACCTCCATA TGAAACCTTT GAAGATTTAC GAGAGAAACT TCTCATGGCC
    GTGGAAAATG CTCAAGGATT TGAAGGGGTG GATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001230889.1
    CDS436..3171
    Translation

    Target ORF information:

    RefSeq Version NM_001243960.2
    Organism Homo sapiens(human)
    Definition Homo sapiens NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant i, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001243960.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCGACCG GGCTCGGGGA GCCGGTCTAT GGACTTTCCG AAGACGAGGG AGAGTCCCGT 
    ATTCTCAGAG TAAAAGTTGT TTCTGGAATT GATCTCGCCA AAAAGGACAT CTTTGGAGCC
    AGTGATCCGT ATGTGAAACT TTCATTGTAC GTAGCGGATG AGAATAGAGA ACTTGCTTTG
    GTCCAGACAA AAACAATTAA AAAGACACTG AACCCAAAAT GGAATGAAGA ATTTTATTTC
    AGGGTAAACC CATCTAATCA CAGACTCCTA TTTGAAGTAT TTGACGAAAA TAGACTGACA
    CGAGACGACT TCCTGGGCCA GGTGGACGTG CCCCTTAGTC ACCTTCCGAC AGAAGATCCA
    ACCATGGAGC GACCCTATAC ATTTAAGGAC TTTCTCCTCA GACCAAGAAG TCATAAGTCT
    CGAGTTAAGG GATTTTTGCG ATTGAAAATG GCCTATATGC CAAAAAATGG AGGTCAAGAT
    GAAGAAAACA GTGACCAGAG GGATGACATG GAGCATGGAT GGGAAGTTGT TGACTCAAAT
    GACTCGGCTT CTCAGCACCA AGAGGAACTT CCTCCTCCTC CTCTGCCTCC CGGGTGGGAA
    GAAAAAGTGG ACAATTTAGG CCGAACTTAC TATGTCAACC ACAACAACCG GACCACTCAG
    TGGCACAGAC CAAGCCTGAT GGACGTGTCC TCGGAGTCGG ACAATAACAT CAGACAGATC
    AACCAGGAGG CAGCACACCG GCGCTTCCGC TCCCGCAGGC ACATCAGCGA AGACTTGGAG
    CCCGAGCCCT CGGAGGGCGG GGATGTCCCC GAGCCTTGGG AGACCATTTC AGAGGAAGTG
    AATATCGCTG GAGACTCTCT CGGTCTGGCT CTGCCCCCAC CACCGGCCTC CCCAGGATCT
    CGGACCAGCC CTCAGGAGCT GTCAGAGGAA CTAAGCAGAA GGCTTCAGAT CACTCCAGAC
    TCCAATGGGG AACAGTTCAG CTCTTTGATT CAAAGAGAAC CCTCCTCAAG GTTGAGGTCA
    TGCAGTGTCA CCGACGCAGT TGCAGAACAG GGCCATCTAC CACCGCTTGC AGAAGATGGT
    GCGTCCGGAT CAGCCACAAA CAGTAACAAC CATCTAATCG AGCCTCAGAT CCGCCGGCCT
    CGTAGCCTCA GCTCGCCAAC AGTAACTTTA TCTGCCCCGC TGGAGGGTGC CAAGGACTCA
    CCCGTACGTC GGGCTGTGAA AGACACCCTT TCCAACCCAC AGTCCCCACA GCCATCACCT
    TACAACTCCC CCAAACCACA ACACAAAGTC ACACAGAGCT TCTTGCCACC CGGCTGGGAA
    ATGAGGATAG CGCCAAACGG CCGGCCCTTC TTCATTGATC ATAACACAAA GACTACAACC
    TGGGAAGATC CACGTTTGAA ATTTCCAGTA CATATGCGGT CAAAGACATC TTTAAACCCC
    AATGACCTTG GCCCCCTTCC TCCTGGCTGG GAAGAAAGAA TTCACTTGGA TGGCCGAACG
    TTTTATATTG ATCATAATAG CAAAATTACT CAGTGGGAAG ACCCAAGACT GCAGAACCCA
    GCTATTACTG GTCCGGCTGT CCCTTACTCC AGAGAATTTA AGCAGAAATA TGACTACTTC
    AGGAAGAAAT TAAAGAAACC TGCTGATATC CCCAATAGGT TTGAAATGAA ACTTCACAGA
    AATAACATAT TTGAAGAGTC CTATCGGAGA ATTATGTCCG TGAAAAGACC AGATGTCCTA
    AAAGCTAGAC TGTGGATTGA GTTTGAATCA GAGAAAGGTC TTGACTATGG GGGTGTGGCC
    AGAGAATGGT TCTTCTTACT GTCCAAAGAG ATGTTCAACC CCTACTACGG CCTCTTTGAG
    TACTCTGCCA CGGACAACTA CACCCTTCAG ATCAACCCTA ATTCAGGCCT CTGTAATGAG
    GATCATTTGT CCTACTTCAC TTTTATTGGA AGAGTTGCTG GTCTGGCCGT ATTTCATGGG
    AAGCTCTTAG ATGGTTTCTT CATTAGACCA TTTTACAAGA TGATGTTGGG AAAGCAGATA
    ACCCTGAATG ACATGGAATC TGTGGATAGT GAATATTACA ACTCTTTGAA ATGGATCCTG
    GAGAATGACC CTACTGAGCT GGACCTCATG TTCTGCATAG ACGAAGAAAA CTTTGGACAG
    ACATATCAAG TGGATTTGAA GCCCAATGGG TCAGAAATAA TGGTCACAAA TGAAAACAAA
    AGGGAATATA TCGACTTAGT CATCCAGTGG AGATTTGTGA ACAGGGTCCA GAAGCAGATG
    AACGCCTTCT TGGAGGGATT CACAGAACTA CTTCCTATTG ATTTGATTAA AATTTTTGAT
    GAAAATGAGC TGGAGTTGCT CATGTGCGGC CTCGGTGATG TGGATGTGAA TGACTGGAGA
    CAGCATTCTA TTTACAAGAA CGGCTACTGC CCAAACCACC CCGTCATTCA GTGGTTCTGG
    AAGGCTGTGC TACTCATGGA CGCCGAAAAG CGTATCCGGT TACTGCAGTT TGTCACAGGG
    ACATCGCGAG TACCTATGAA TGGATTTGCC GAACTTTATG GTTCCAATGG TCCTCAGCTG
    TTTACAATAG AGCAATGGGG CAGTCCTGAG AAACTGCCCA GAGCTCACAC ATGCTTTAAT
    CGCCTTGACT TACCTCCATA TGAAACCTTT GAAGATTTAC GAGAGAAACT TCTCATGGCC
    GTGGAAAATG CTCAAGGATT TGAAGGGGTG GATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu14742
    Clone ID Related Accession (Same CDS sequence) NM_015277.5 , NM_015277.6
    Accession Version NM_015277.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2868bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-03
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC107896.7, AB071179.1, BC000621.2 and AC090236.4. On Sep 3, 2011 this sequence version replaced NM_015277.4. Transcript Variant: This variant (d) lacks an in-frame exon, compared to variant j, resulting in a shorter protein (isoform 3), compared to isoform 1. Variant d is also known as NEDD4-2C6 and NEDL3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.129494.1, SRR1803614.13808.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGCGACCG GGCTCGGGGA GCCGGTCTAT GGACTTTCCG AAGACGAGGG AGAGTCCCGT 
    ATTCTCAGAG TAAAAGTTGT TTCTGGAATT GATCTCGCCA AAAAGGACAT CTTTGGAGCC
    AGTGATCCGT ATGTGAAACT TTCATTGTAC GTAGCGGATG AGAATAGAGA ACTTGCTTTG
    GTCCAGACAA AAACAATTAA AAAGACACTG AACCCAAAAT GGAATGAAGA ATTTTATTTC
    AGGGTAAACC CATCTAATCA CAGACTCCTA TTTGAAGTAT TTGACGAAAA TAGACTGACA
    CGAGACGACT TCCTGGGCCA GGTGGACGTG CCCCTTAGTC ACCTTCCGAC AGAAGATCCA
    ACCATGGAGC GACCCTATAC ATTTAAGGAC TTTCTCCTCA GACCAAGAAG TCATAAGTCT
    CGAGTTAAGG GATTTTTGCG ATTGAAAATG GCCTATATGC CAAAAAATGG AGGTCAAGAT
    GAAGAAAACA GTGACCAGAG GGATGACATG GAGCATGGAT GGGAAGTTGT TGACTCAAAT
    GACTCGGCTT CTCAGCACCA AGAGGAACTT CCTCCTCCTC CTCTGCCTCC CGGGTGGGAA
    GAAAAAGTGG ACAATTTAGG CCGAACTTAC TATGTCAACC ACAACAACCG GACCACTCAG
    TGGCACAGAC CAAGCCTGAT GGACGTGTCC TCGGAGTCGG ACAATAACAT CAGACAGATC
    AACCAGGAGG CAGCACACCG GCGCTTCCGC TCCCGCAGGC ACATCAGCGA AGACTTGGAG
    CCCGAGCCCT CGGAGGGCGG GGATGTCCCC GAGCCTTGGG AGACCATTTC AGAGGAAGTG
    AATATCGCTG GAGACTCTCT CGGTCTGGCT CTGCCCCCAC CACCGGCCTC CCCAGGATCT
    CGGACCAGCC CTCAGGAGCT GTCAGAGGAA CTAAGCAGAA GGCTTCAGAT CACTCCAGAC
    TCCAATGGGG AACAGTTCAG CTCTTTGATT CAAAGAGAAC CCTCCTCAAG GTTGAGGTCA
    TGCAGTGTCA CCGACGCAGT TGCAGAACAG GGCCATCTAC CACCGCCATC AGTGGCCTAT
    GTACATACCA CGCCGGGTCT GCCTTCAGGC TGGGAAGAAA GAAAAGATGC TAAGGGGCGC
    ACATACTATG TCAATCATAA CAATCGAACC ACAACTTGGA CTCGACCTAT CATGCAGCTT
    GCAGAAGATG GTGCGTCCGG ATCAGCCACA AACAGTAACA ACCATCTAAT CGAGCCTCAG
    ATCCGCCGGC CTCGTAGCCT CAGCTCGCCA ACAGTAACTT TATCTGCCCC GCTGGAGGGT
    GCCAAGGACT CACCCGTACG TCGGGCTGTG AAAGACACCC TTTCCAACCC ACAGTCCCCA
    CAGCCATCAC CTTACAACTC CCCCAAACCA CAACACAAAG TCACACAGAG CTTCTTGCCA
    CCCGGCTGGG AAATGAGGAT AGCGCCAAAC GGCCGGCCCT TCTTCATTGA TCATAACACA
    AAGACTACAA CCTGGGAAGA TCCACGTTTG AAATTTCCAG TACATATGCG GTCAAAGACA
    TCTTTAAACC CCAATGACCT TGGCCCCCTT CCTCCTGGCT GGGAAGAAAG AATTCACTTG
    GATGGCCGAA CGTTTTATAT TGATCATAAT AGCAAAATTA CTCAGTGGGA AGACCCAAGA
    CTGCAGAACC CAGCTATTAC TGGTCCGGCT GTCCCTTACT CCAGAGAATT TAAGCAGAAA
    TATGACTACT TCAGGAAGAA ATTAAAGAAA CCTGCTGATA TCCCCAATAG GTTTGAAATG
    AAACTTCACA GAAATAACAT ATTTGAAGAG TCCTATCGGA GAATTATGTC CGTGAAAAGA
    CCAGATGTCC TAAAAGCTAG ACTGTGGATT GAGTTTGAAT CAGAGAAAGG TCTTGACTAT
    GGGGGTGTGG CCAGAGAATG GTTCTTCTTA CTGTCCAAAG AGATGTTCAA CCCCTACTAC
    GGCCTCTTTG AGTACTCTGC CACGGACAAC TACACCCTTC AGATCAACCC TAATTCAGGC
    CTCTGTAATG AGGATCATTT GTCCTACTTC ACTTTTATTG GAAGAGTTGC TGGTCTGGCC
    GTATTTCATG GGAAGCTCTT AGATGGTTTC TTCATTAGAC CATTTTACAA GATGATGTTG
    GGAAAGCAGA TAACCCTGAA TGACATGGAA TCTGTGGATA GTGAATATTA CAACTCTTTG
    AAATGGATCC TGGAGAATGA CCCTACTGAG CTGGACCTCA TGTTCTGCAT AGACGAAGAA
    AACTTTGGAC AGACATATCA AGTGGATTTG AAGCCCAATG GGTCAGAAAT AATGGTCACA
    AATGAAAACA AAAGGGAATA TATCGACTTA GTCATCCAGT GGAGATTTGT GAACAGGGTC
    CAGAAGCAGA TGAACGCCTT CTTGGAGGGA TTCACAGAAC TACTTCCTAT TGATTTGATT
    AAAATTTTTG ATGAAAATGA GCTGGAGTTG CTCATGTGCG GCCTCGGTGA TGTGGATGTG
    AATGACTGGA GACAGCATTC TATTTACAAG AACGGCTACT GCCCAAACCA CCCCGTCATT
    CAGTGGTTCT GGAAGGCTGT GCTACTCATG GACGCCGAAA AGCGTATCCG GTTACTGCAG
    TTTGTCACAG GGACATCGCG AGTACCTATG AATGGATTTG CCGAACTTTA TGGTTCCAAT
    GGTCCTCAGC TGTTTACAAT AGAGCAATGG GGCAGTCCTG AGAAACTGCC CAGAGCTCAC
    ACATGCTTTA ATCGCCTTGA CTTACCTCCA TATGAAACCT TTGAAGATTT ACGAGAGAAA
    CTTCTCATGG CCGTGGAAAA TGCTCAAGGA TTTGAAGGGG TGGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_056092.2
    CDS284..3151
    Translation

    Target ORF information:

    RefSeq Version NM_015277.5
    Organism Homo sapiens(human)
    Definition Homo sapiens neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant d, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_015277.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGCGACCG GGCTCGGGGA GCCGGTCTAT GGACTTTCCG AAGACGAGGG AGAGTCCCGT 
    ATTCTCAGAG TAAAAGTTGT TTCTGGAATT GATCTCGCCA AAAAGGACAT CTTTGGAGCC
    AGTGATCCGT ATGTGAAACT TTCATTGTAC GTAGCGGATG AGAATAGAGA ACTTGCTTTG
    GTCCAGACAA AAACAATTAA AAAGACACTG AACCCAAAAT GGAATGAAGA ATTTTATTTC
    AGGGTAAACC CATCTAATCA CAGACTCCTA TTTGAAGTAT TTGACGAAAA TAGACTGACA
    CGAGACGACT TCCTGGGCCA GGTGGACGTG CCCCTTAGTC ACCTTCCGAC AGAAGATCCA
    ACCATGGAGC GACCCTATAC ATTTAAGGAC TTTCTCCTCA GACCAAGAAG TCATAAGTCT
    CGAGTTAAGG GATTTTTGCG ATTGAAAATG GCCTATATGC CAAAAAATGG AGGTCAAGAT
    GAAGAAAACA GTGACCAGAG GGATGACATG GAGCATGGAT GGGAAGTTGT TGACTCAAAT
    GACTCGGCTT CTCAGCACCA AGAGGAACTT CCTCCTCCTC CTCTGCCTCC CGGGTGGGAA
    GAAAAAGTGG ACAATTTAGG CCGAACTTAC TATGTCAACC ACAACAACCG GACCACTCAG
    TGGCACAGAC CAAGCCTGAT GGACGTGTCC TCGGAGTCGG ACAATAACAT CAGACAGATC
    AACCAGGAGG CAGCACACCG GCGCTTCCGC TCCCGCAGGC ACATCAGCGA AGACTTGGAG
    CCCGAGCCCT CGGAGGGCGG GGATGTCCCC GAGCCTTGGG AGACCATTTC AGAGGAAGTG
    AATATCGCTG GAGACTCTCT CGGTCTGGCT CTGCCCCCAC CACCGGCCTC CCCAGGATCT
    CGGACCAGCC CTCAGGAGCT GTCAGAGGAA CTAAGCAGAA GGCTTCAGAT CACTCCAGAC
    TCCAATGGGG AACAGTTCAG CTCTTTGATT CAAAGAGAAC CCTCCTCAAG GTTGAGGTCA
    TGCAGTGTCA CCGACGCAGT TGCAGAACAG GGCCATCTAC CACCGCCATC AGTGGCCTAT
    GTACATACCA CGCCGGGTCT GCCTTCAGGC TGGGAAGAAA GAAAAGATGC TAAGGGGCGC
    ACATACTATG TCAATCATAA CAATCGAACC ACAACTTGGA CTCGACCTAT CATGCAGCTT
    GCAGAAGATG GTGCGTCCGG ATCAGCCACA AACAGTAACA ACCATCTAAT CGAGCCTCAG
    ATCCGCCGGC CTCGTAGCCT CAGCTCGCCA ACAGTAACTT TATCTGCCCC GCTGGAGGGT
    GCCAAGGACT CACCCGTACG TCGGGCTGTG AAAGACACCC TTTCCAACCC ACAGTCCCCA
    CAGCCATCAC CTTACAACTC CCCCAAACCA CAACACAAAG TCACACAGAG CTTCTTGCCA
    CCCGGCTGGG AAATGAGGAT AGCGCCAAAC GGCCGGCCCT TCTTCATTGA TCATAACACA
    AAGACTACAA CCTGGGAAGA TCCACGTTTG AAATTTCCAG TACATATGCG GTCAAAGACA
    TCTTTAAACC CCAATGACCT TGGCCCCCTT CCTCCTGGCT GGGAAGAAAG AATTCACTTG
    GATGGCCGAA CGTTTTATAT TGATCATAAT AGCAAAATTA CTCAGTGGGA AGACCCAAGA
    CTGCAGAACC CAGCTATTAC TGGTCCGGCT GTCCCTTACT CCAGAGAATT TAAGCAGAAA
    TATGACTACT TCAGGAAGAA ATTAAAGAAA CCTGCTGATA TCCCCAATAG GTTTGAAATG
    AAACTTCACA GAAATAACAT ATTTGAAGAG TCCTATCGGA GAATTATGTC CGTGAAAAGA
    CCAGATGTCC TAAAAGCTAG ACTGTGGATT GAGTTTGAAT CAGAGAAAGG TCTTGACTAT
    GGGGGTGTGG CCAGAGAATG GTTCTTCTTA CTGTCCAAAG AGATGTTCAA CCCCTACTAC
    GGCCTCTTTG AGTACTCTGC CACGGACAAC TACACCCTTC AGATCAACCC TAATTCAGGC
    CTCTGTAATG AGGATCATTT GTCCTACTTC ACTTTTATTG GAAGAGTTGC TGGTCTGGCC
    GTATTTCATG GGAAGCTCTT AGATGGTTTC TTCATTAGAC CATTTTACAA GATGATGTTG
    GGAAAGCAGA TAACCCTGAA TGACATGGAA TCTGTGGATA GTGAATATTA CAACTCTTTG
    AAATGGATCC TGGAGAATGA CCCTACTGAG CTGGACCTCA TGTTCTGCAT AGACGAAGAA
    AACTTTGGAC AGACATATCA AGTGGATTTG AAGCCCAATG GGTCAGAAAT AATGGTCACA
    AATGAAAACA AAAGGGAATA TATCGACTTA GTCATCCAGT GGAGATTTGT GAACAGGGTC
    CAGAAGCAGA TGAACGCCTT CTTGGAGGGA TTCACAGAAC TACTTCCTAT TGATTTGATT
    AAAATTTTTG ATGAAAATGA GCTGGAGTTG CTCATGTGCG GCCTCGGTGA TGTGGATGTG
    AATGACTGGA GACAGCATTC TATTTACAAG AACGGCTACT GCCCAAACCA CCCCGTCATT
    CAGTGGTTCT GGAAGGCTGT GCTACTCATG GACGCCGAAA AGCGTATCCG GTTACTGCAG
    TTTGTCACAG GGACATCGCG AGTACCTATG AATGGATTTG CCGAACTTTA TGGTTCCAAT
    GGTCCTCAGC TGTTTACAAT AGAGCAATGG GGCAGTCCTG AGAAACTGCC CAGAGCTCAC
    ACATGCTTTA ATCGCCTTGA CTTACCTCCA TATGAAACCT TTGAAGATTT ACGAGAGAAA
    CTTCTCATGG CCGTGGAAAA TGCTCAAGGA TTTGAAGGGG TGGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu14742
    Clone ID Related Accession (Same CDS sequence) NM_015277.5 , NM_015277.6
    Accession Version NM_015277.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2868bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-20
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC107896.7, AB071179.1, BC000621.2 and AC090236.4. On May 31, 2019 this sequence version replaced NM_015277.5. Transcript Variant: This variant (d) lacks an in-frame exon, compared to variant j, resulting in a shorter protein (isoform 3), compared to isoform 1. Variant d is also known as NEDD4-2C6 and NEDL3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.129494.1, AB071179.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGCGACCG GGCTCGGGGA GCCGGTCTAT GGACTTTCCG AAGACGAGGG AGAGTCCCGT 
    ATTCTCAGAG TAAAAGTTGT TTCTGGAATT GATCTCGCCA AAAAGGACAT CTTTGGAGCC
    AGTGATCCGT ATGTGAAACT TTCATTGTAC GTAGCGGATG AGAATAGAGA ACTTGCTTTG
    GTCCAGACAA AAACAATTAA AAAGACACTG AACCCAAAAT GGAATGAAGA ATTTTATTTC
    AGGGTAAACC CATCTAATCA CAGACTCCTA TTTGAAGTAT TTGACGAAAA TAGACTGACA
    CGAGACGACT TCCTGGGCCA GGTGGACGTG CCCCTTAGTC ACCTTCCGAC AGAAGATCCA
    ACCATGGAGC GACCCTATAC ATTTAAGGAC TTTCTCCTCA GACCAAGAAG TCATAAGTCT
    CGAGTTAAGG GATTTTTGCG ATTGAAAATG GCCTATATGC CAAAAAATGG AGGTCAAGAT
    GAAGAAAACA GTGACCAGAG GGATGACATG GAGCATGGAT GGGAAGTTGT TGACTCAAAT
    GACTCGGCTT CTCAGCACCA AGAGGAACTT CCTCCTCCTC CTCTGCCTCC CGGGTGGGAA
    GAAAAAGTGG ACAATTTAGG CCGAACTTAC TATGTCAACC ACAACAACCG GACCACTCAG
    TGGCACAGAC CAAGCCTGAT GGACGTGTCC TCGGAGTCGG ACAATAACAT CAGACAGATC
    AACCAGGAGG CAGCACACCG GCGCTTCCGC TCCCGCAGGC ACATCAGCGA AGACTTGGAG
    CCCGAGCCCT CGGAGGGCGG GGATGTCCCC GAGCCTTGGG AGACCATTTC AGAGGAAGTG
    AATATCGCTG GAGACTCTCT CGGTCTGGCT CTGCCCCCAC CACCGGCCTC CCCAGGATCT
    CGGACCAGCC CTCAGGAGCT GTCAGAGGAA CTAAGCAGAA GGCTTCAGAT CACTCCAGAC
    TCCAATGGGG AACAGTTCAG CTCTTTGATT CAAAGAGAAC CCTCCTCAAG GTTGAGGTCA
    TGCAGTGTCA CCGACGCAGT TGCAGAACAG GGCCATCTAC CACCGCCATC AGTGGCCTAT
    GTACATACCA CGCCGGGTCT GCCTTCAGGC TGGGAAGAAA GAAAAGATGC TAAGGGGCGC
    ACATACTATG TCAATCATAA CAATCGAACC ACAACTTGGA CTCGACCTAT CATGCAGCTT
    GCAGAAGATG GTGCGTCCGG ATCAGCCACA AACAGTAACA ACCATCTAAT CGAGCCTCAG
    ATCCGCCGGC CTCGTAGCCT CAGCTCGCCA ACAGTAACTT TATCTGCCCC GCTGGAGGGT
    GCCAAGGACT CACCCGTACG TCGGGCTGTG AAAGACACCC TTTCCAACCC ACAGTCCCCA
    CAGCCATCAC CTTACAACTC CCCCAAACCA CAACACAAAG TCACACAGAG CTTCTTGCCA
    CCCGGCTGGG AAATGAGGAT AGCGCCAAAC GGCCGGCCCT TCTTCATTGA TCATAACACA
    AAGACTACAA CCTGGGAAGA TCCACGTTTG AAATTTCCAG TACATATGCG GTCAAAGACA
    TCTTTAAACC CCAATGACCT TGGCCCCCTT CCTCCTGGCT GGGAAGAAAG AATTCACTTG
    GATGGCCGAA CGTTTTATAT TGATCATAAT AGCAAAATTA CTCAGTGGGA AGACCCAAGA
    CTGCAGAACC CAGCTATTAC TGGTCCGGCT GTCCCTTACT CCAGAGAATT TAAGCAGAAA
    TATGACTACT TCAGGAAGAA ATTAAAGAAA CCTGCTGATA TCCCCAATAG GTTTGAAATG
    AAACTTCACA GAAATAACAT ATTTGAAGAG TCCTATCGGA GAATTATGTC CGTGAAAAGA
    CCAGATGTCC TAAAAGCTAG ACTGTGGATT GAGTTTGAAT CAGAGAAAGG TCTTGACTAT
    GGGGGTGTGG CCAGAGAATG GTTCTTCTTA CTGTCCAAAG AGATGTTCAA CCCCTACTAC
    GGCCTCTTTG AGTACTCTGC CACGGACAAC TACACCCTTC AGATCAACCC TAATTCAGGC
    CTCTGTAATG AGGATCATTT GTCCTACTTC ACTTTTATTG GAAGAGTTGC TGGTCTGGCC
    GTATTTCATG GGAAGCTCTT AGATGGTTTC TTCATTAGAC CATTTTACAA GATGATGTTG
    GGAAAGCAGA TAACCCTGAA TGACATGGAA TCTGTGGATA GTGAATATTA CAACTCTTTG
    AAATGGATCC TGGAGAATGA CCCTACTGAG CTGGACCTCA TGTTCTGCAT AGACGAAGAA
    AACTTTGGAC AGACATATCA AGTGGATTTG AAGCCCAATG GGTCAGAAAT AATGGTCACA
    AATGAAAACA AAAGGGAATA TATCGACTTA GTCATCCAGT GGAGATTTGT GAACAGGGTC
    CAGAAGCAGA TGAACGCCTT CTTGGAGGGA TTCACAGAAC TACTTCCTAT TGATTTGATT
    AAAATTTTTG ATGAAAATGA GCTGGAGTTG CTCATGTGCG GCCTCGGTGA TGTGGATGTG
    AATGACTGGA GACAGCATTC TATTTACAAG AACGGCTACT GCCCAAACCA CCCCGTCATT
    CAGTGGTTCT GGAAGGCTGT GCTACTCATG GACGCCGAAA AGCGTATCCG GTTACTGCAG
    TTTGTCACAG GGACATCGCG AGTACCTATG AATGGATTTG CCGAACTTTA TGGTTCCAAT
    GGTCCTCAGC TGTTTACAAT AGAGCAATGG GGCAGTCCTG AGAAACTGCC CAGAGCTCAC
    ACATGCTTTA ATCGCCTTGA CTTACCTCCA TATGAAACCT TTGAAGATTT ACGAGAGAAA
    CTTCTCATGG CCGTGGAAAA TGCTCAAGGA TTTGAAGGGG TGGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_056092.2
    CDS436..3303
    Translation

    Target ORF information:

    RefSeq Version NM_015277.6
    Organism Homo sapiens(human)
    Definition Homo sapiens NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant d, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_015277.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGCGACCG GGCTCGGGGA GCCGGTCTAT GGACTTTCCG AAGACGAGGG AGAGTCCCGT 
    ATTCTCAGAG TAAAAGTTGT TTCTGGAATT GATCTCGCCA AAAAGGACAT CTTTGGAGCC
    AGTGATCCGT ATGTGAAACT TTCATTGTAC GTAGCGGATG AGAATAGAGA ACTTGCTTTG
    GTCCAGACAA AAACAATTAA AAAGACACTG AACCCAAAAT GGAATGAAGA ATTTTATTTC
    AGGGTAAACC CATCTAATCA CAGACTCCTA TTTGAAGTAT TTGACGAAAA TAGACTGACA
    CGAGACGACT TCCTGGGCCA GGTGGACGTG CCCCTTAGTC ACCTTCCGAC AGAAGATCCA
    ACCATGGAGC GACCCTATAC ATTTAAGGAC TTTCTCCTCA GACCAAGAAG TCATAAGTCT
    CGAGTTAAGG GATTTTTGCG ATTGAAAATG GCCTATATGC CAAAAAATGG AGGTCAAGAT
    GAAGAAAACA GTGACCAGAG GGATGACATG GAGCATGGAT GGGAAGTTGT TGACTCAAAT
    GACTCGGCTT CTCAGCACCA AGAGGAACTT CCTCCTCCTC CTCTGCCTCC CGGGTGGGAA
    GAAAAAGTGG ACAATTTAGG CCGAACTTAC TATGTCAACC ACAACAACCG GACCACTCAG
    TGGCACAGAC CAAGCCTGAT GGACGTGTCC TCGGAGTCGG ACAATAACAT CAGACAGATC
    AACCAGGAGG CAGCACACCG GCGCTTCCGC TCCCGCAGGC ACATCAGCGA AGACTTGGAG
    CCCGAGCCCT CGGAGGGCGG GGATGTCCCC GAGCCTTGGG AGACCATTTC AGAGGAAGTG
    AATATCGCTG GAGACTCTCT CGGTCTGGCT CTGCCCCCAC CACCGGCCTC CCCAGGATCT
    CGGACCAGCC CTCAGGAGCT GTCAGAGGAA CTAAGCAGAA GGCTTCAGAT CACTCCAGAC
    TCCAATGGGG AACAGTTCAG CTCTTTGATT CAAAGAGAAC CCTCCTCAAG GTTGAGGTCA
    TGCAGTGTCA CCGACGCAGT TGCAGAACAG GGCCATCTAC CACCGCCATC AGTGGCCTAT
    GTACATACCA CGCCGGGTCT GCCTTCAGGC TGGGAAGAAA GAAAAGATGC TAAGGGGCGC
    ACATACTATG TCAATCATAA CAATCGAACC ACAACTTGGA CTCGACCTAT CATGCAGCTT
    GCAGAAGATG GTGCGTCCGG ATCAGCCACA AACAGTAACA ACCATCTAAT CGAGCCTCAG
    ATCCGCCGGC CTCGTAGCCT CAGCTCGCCA ACAGTAACTT TATCTGCCCC GCTGGAGGGT
    GCCAAGGACT CACCCGTACG TCGGGCTGTG AAAGACACCC TTTCCAACCC ACAGTCCCCA
    CAGCCATCAC CTTACAACTC CCCCAAACCA CAACACAAAG TCACACAGAG CTTCTTGCCA
    CCCGGCTGGG AAATGAGGAT AGCGCCAAAC GGCCGGCCCT TCTTCATTGA TCATAACACA
    AAGACTACAA CCTGGGAAGA TCCACGTTTG AAATTTCCAG TACATATGCG GTCAAAGACA
    TCTTTAAACC CCAATGACCT TGGCCCCCTT CCTCCTGGCT GGGAAGAAAG AATTCACTTG
    GATGGCCGAA CGTTTTATAT TGATCATAAT AGCAAAATTA CTCAGTGGGA AGACCCAAGA
    CTGCAGAACC CAGCTATTAC TGGTCCGGCT GTCCCTTACT CCAGAGAATT TAAGCAGAAA
    TATGACTACT TCAGGAAGAA ATTAAAGAAA CCTGCTGATA TCCCCAATAG GTTTGAAATG
    AAACTTCACA GAAATAACAT ATTTGAAGAG TCCTATCGGA GAATTATGTC CGTGAAAAGA
    CCAGATGTCC TAAAAGCTAG ACTGTGGATT GAGTTTGAAT CAGAGAAAGG TCTTGACTAT
    GGGGGTGTGG CCAGAGAATG GTTCTTCTTA CTGTCCAAAG AGATGTTCAA CCCCTACTAC
    GGCCTCTTTG AGTACTCTGC CACGGACAAC TACACCCTTC AGATCAACCC TAATTCAGGC
    CTCTGTAATG AGGATCATTT GTCCTACTTC ACTTTTATTG GAAGAGTTGC TGGTCTGGCC
    GTATTTCATG GGAAGCTCTT AGATGGTTTC TTCATTAGAC CATTTTACAA GATGATGTTG
    GGAAAGCAGA TAACCCTGAA TGACATGGAA TCTGTGGATA GTGAATATTA CAACTCTTTG
    AAATGGATCC TGGAGAATGA CCCTACTGAG CTGGACCTCA TGTTCTGCAT AGACGAAGAA
    AACTTTGGAC AGACATATCA AGTGGATTTG AAGCCCAATG GGTCAGAAAT AATGGTCACA
    AATGAAAACA AAAGGGAATA TATCGACTTA GTCATCCAGT GGAGATTTGT GAACAGGGTC
    CAGAAGCAGA TGAACGCCTT CTTGGAGGGA TTCACAGAAC TACTTCCTAT TGATTTGATT
    AAAATTTTTG ATGAAAATGA GCTGGAGTTG CTCATGTGCG GCCTCGGTGA TGTGGATGTG
    AATGACTGGA GACAGCATTC TATTTACAAG AACGGCTACT GCCCAAACCA CCCCGTCATT
    CAGTGGTTCT GGAAGGCTGT GCTACTCATG GACGCCGAAA AGCGTATCCG GTTACTGCAG
    TTTGTCACAG GGACATCGCG AGTACCTATG AATGGATTTG CCGAACTTTA TGGTTCCAAT
    GGTCCTCAGC TGTTTACAAT AGAGCAATGG GGCAGTCCTG AGAAACTGCC CAGAGCTCAC
    ACATGCTTTA ATCGCCTTGA CTTACCTCCA TATGAAACCT TTGAAGATTT ACGAGAGAAA
    CTTCTCATGG CCGTGGAAAA TGCTCAAGGA TTTGAAGGGG TGGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu14254
    Clone ID Related Accession (Same CDS sequence) XM_024451136.1 , XM_024451137.1 , NM_001144971.2 , NM_001144970.3 , NM_001144971.1 , NM_001144970.2 , XM_024451135.1 , XM_024451131.1
    Accession Version XM_024451136.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2505bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGAGCGAC CCTATACATT TAAGGACTTT CTCCTCAGAC CAAGAAGTCA TAAGTCTCGA 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCAA AAAATGGAGG TCAAGATGAA
    GAAAACAGTG ACCAGAGGGA TGACATGGAG CATGGATGGG AAGTTGTTGA CTCAAATGAC
    TCGGCTTCTC AGCACCAAGA GGAACTTCCT CCTCCTCCTC TGCCTCCCGG GTGGGAAGAA
    AAAGTGGACA ATTTAGGCCG AACTTACTAT GTCAACCACA ACAACCGGAC CACTCAGTGG
    CACAGACCAA GCCTGATGGA CGTGTCCTCG GAGTCGGACA ATAACATCAG ACAGATCAAC
    CAGGAGGCAG CACACCGGCG CTTCCGCTCC CGCAGGCACA TCAGCGAAGA CTTGGAGCCC
    GAGCCCTCGG AGGGCGGGGA TGTCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAT
    ATCGCTGGAG ACTCTCTCGG TCTGGCTCTG CCCCCACCAC CGGCCTCCCC AGGATCTCGG
    ACCAGCCCTC AGGAGCTGTC AGAGGAACTA AGCAGAAGGC TTCAGATCAC TCCAGACTCC
    AATGGGGAAC AGTTCAGCTC TTTGATTCAA AGAGAACCCT CCTCAAGGTT GAGGTCATGC
    AGTGTCACCG ACGCAGTTGC AGAACAGGGC CATCTACCAC CGCCATCAGT GGCCTATGTA
    CATACCACGC CGGGTCTGCC TTCAGGCTGG GAAGAAAGAA AAGATGCTAA GGGGCGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GACCTATCAT GCAGCTTGCA
    GAAGATGGTG CGTCCGGATC AGCCACAAAC AGTAACAACC ATCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CTGCCCCGCT GGAGGGTGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GACACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CTTGCCACCC
    GGCTGGGAAA TGAGGATAGC GCCAAACGGC CGGCCCTTCT TCATTGATCA TAACACAAAG
    ACTACAACCT GGGAAGATCC ACGTTTGAAA TTTCCAGTAC ATATGCGGTC AAAGACATCT
    TTAAACCCCA ATGACCTTGG CCCCCTTCCT CCTGGCTGGG AAGAAAGAAT TCACTTGGAT
    GGCCGAACGT TTTATATTGA TCATAATAGC AAAATTACTC AGTGGGAAGA CCCAAGACTG
    CAGAACCCAG CTATTACTGG TCCGGCTGTC CCTTACTCCA GAGAATTTAA GCAGAAATAT
    GACTACTTCA GGAAGAAATT AAAGAAACCT GCTGATATCC CCAATAGGTT TGAAATGAAA
    CTTCACAGAA ATAACATATT TGAAGAGTCC TATCGGAGAA TTATGTCCGT GAAAAGACCA
    GATGTCCTAA AAGCTAGACT GTGGATTGAG TTTGAATCAG AGAAAGGTCT TGACTATGGG
    GGTGTGGCCA GAGAATGGTT CTTCTTACTG TCCAAAGAGA TGTTCAACCC CTACTACGGC
    CTCTTTGAGT ACTCTGCCAC GGACAACTAC ACCCTTCAGA TCAACCCTAA TTCAGGCCTC
    TGTAATGAGG ATCATTTGTC CTACTTCACT TTTATTGGAA GAGTTGCTGG TCTGGCCGTA
    TTTCATGGGA AGCTCTTAGA TGGTTTCTTC ATTAGACCAT TTTACAAGAT GATGTTGGGA
    AAGCAGATAA CCCTGAATGA CATGGAATCT GTGGATAGTG AATATTACAA CTCTTTGAAA
    TGGATCCTGG AGAATGACCC TACTGAGCTG GACCTCATGT TCTGCATAGA CGAAGAAAAC
    TTTGGACAGA CATATCAAGT GGATTTGAAG CCCAATGGGT CAGAAATAAT GGTCACAAAT
    GAAAACAAAA GGGAATATAT CGACTTAGTC ATCCAGTGGA GATTTGTGAA CAGGGTCCAG
    AAGCAGATGA ACGCCTTCTT GGAGGGATTC ACAGAACTAC TTCCTATTGA TTTGATTAAA
    ATTTTTGATG AAAATGAGCT GGAGTTGCTC ATGTGCGGCC TCGGTGATGT GGATGTGAAT
    GACTGGAGAC AGCATTCTAT TTACAAGAAC GGCTACTGCC CAAACCACCC CGTCATTCAG
    TGGTTCTGGA AGGCTGTGCT ACTCATGGAC GCCGAAAAGC GTATCCGGTT ACTGCAGTTT
    GTCACAGGGA CATCGCGAGT ACCTATGAAT GGATTTGCCG AACTTTATGG TTCCAATGGT
    CCTCAGCTGT TTACAATAGA GCAATGGGGC AGTCCTGAGA AACTGCCCAG AGCTCACACA
    TGCTTTAATC GCCTTGACTT ACCTCCATAT GAAACCTTTG AAGATTTACG AGAGAAACTT
    CTCATGGCCG TGGAAAATGC TCAAGGATTT GAAGGGGTGG ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024306904.1
    CDS392..2896
    Translation

    Target ORF information:

    RefSeq Version XM_024451136.1
    Organism Homo sapiens(human)
    Definition Homo sapiens NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant X22, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024451136.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGAGCGAC CCTATACATT TAAGGACTTT CTCCTCAGAC CAAGAAGTCA TAAGTCTCGA 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCAA AAAATGGAGG TCAAGATGAA
    GAAAACAGTG ACCAGAGGGA TGACATGGAG CATGGATGGG AAGTTGTTGA CTCAAATGAC
    TCGGCTTCTC AGCACCAAGA GGAACTTCCT CCTCCTCCTC TGCCTCCCGG GTGGGAAGAA
    AAAGTGGACA ATTTAGGCCG AACTTACTAT GTCAACCACA ACAACCGGAC CACTCAGTGG
    CACAGACCAA GCCTGATGGA CGTGTCCTCG GAGTCGGACA ATAACATCAG ACAGATCAAC
    CAGGAGGCAG CACACCGGCG CTTCCGCTCC CGCAGGCACA TCAGCGAAGA CTTGGAGCCC
    GAGCCCTCGG AGGGCGGGGA TGTCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAT
    ATCGCTGGAG ACTCTCTCGG TCTGGCTCTG CCCCCACCAC CGGCCTCCCC AGGATCTCGG
    ACCAGCCCTC AGGAGCTGTC AGAGGAACTA AGCAGAAGGC TTCAGATCAC TCCAGACTCC
    AATGGGGAAC AGTTCAGCTC TTTGATTCAA AGAGAACCCT CCTCAAGGTT GAGGTCATGC
    AGTGTCACCG ACGCAGTTGC AGAACAGGGC CATCTACCAC CGCCATCAGT GGCCTATGTA
    CATACCACGC CGGGTCTGCC TTCAGGCTGG GAAGAAAGAA AAGATGCTAA GGGGCGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GACCTATCAT GCAGCTTGCA
    GAAGATGGTG CGTCCGGATC AGCCACAAAC AGTAACAACC ATCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CTGCCCCGCT GGAGGGTGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GACACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CTTGCCACCC
    GGCTGGGAAA TGAGGATAGC GCCAAACGGC CGGCCCTTCT TCATTGATCA TAACACAAAG
    ACTACAACCT GGGAAGATCC ACGTTTGAAA TTTCCAGTAC ATATGCGGTC AAAGACATCT
    TTAAACCCCA ATGACCTTGG CCCCCTTCCT CCTGGCTGGG AAGAAAGAAT TCACTTGGAT
    GGCCGAACGT TTTATATTGA TCATAATAGC AAAATTACTC AGTGGGAAGA CCCAAGACTG
    CAGAACCCAG CTATTACTGG TCCGGCTGTC CCTTACTCCA GAGAATTTAA GCAGAAATAT
    GACTACTTCA GGAAGAAATT AAAGAAACCT GCTGATATCC CCAATAGGTT TGAAATGAAA
    CTTCACAGAA ATAACATATT TGAAGAGTCC TATCGGAGAA TTATGTCCGT GAAAAGACCA
    GATGTCCTAA AAGCTAGACT GTGGATTGAG TTTGAATCAG AGAAAGGTCT TGACTATGGG
    GGTGTGGCCA GAGAATGGTT CTTCTTACTG TCCAAAGAGA TGTTCAACCC CTACTACGGC
    CTCTTTGAGT ACTCTGCCAC GGACAACTAC ACCCTTCAGA TCAACCCTAA TTCAGGCCTC
    TGTAATGAGG ATCATTTGTC CTACTTCACT TTTATTGGAA GAGTTGCTGG TCTGGCCGTA
    TTTCATGGGA AGCTCTTAGA TGGTTTCTTC ATTAGACCAT TTTACAAGAT GATGTTGGGA
    AAGCAGATAA CCCTGAATGA CATGGAATCT GTGGATAGTG AATATTACAA CTCTTTGAAA
    TGGATCCTGG AGAATGACCC TACTGAGCTG GACCTCATGT TCTGCATAGA CGAAGAAAAC
    TTTGGACAGA CATATCAAGT GGATTTGAAG CCCAATGGGT CAGAAATAAT GGTCACAAAT
    GAAAACAAAA GGGAATATAT CGACTTAGTC ATCCAGTGGA GATTTGTGAA CAGGGTCCAG
    AAGCAGATGA ACGCCTTCTT GGAGGGATTC ACAGAACTAC TTCCTATTGA TTTGATTAAA
    ATTTTTGATG AAAATGAGCT GGAGTTGCTC ATGTGCGGCC TCGGTGATGT GGATGTGAAT
    GACTGGAGAC AGCATTCTAT TTACAAGAAC GGCTACTGCC CAAACCACCC CGTCATTCAG
    TGGTTCTGGA AGGCTGTGCT ACTCATGGAC GCCGAAAAGC GTATCCGGTT ACTGCAGTTT
    GTCACAGGGA CATCGCGAGT ACCTATGAAT GGATTTGCCG AACTTTATGG TTCCAATGGT
    CCTCAGCTGT TTACAATAGA GCAATGGGGC AGTCCTGAGA AACTGCCCAG AGCTCACACA
    TGCTTTAATC GCCTTGACTT ACCTCCATAT GAAACCTTTG AAGATTTACG AGAGAAACTT
    CTCATGGCCG TGGAAAATGC TCAAGGATTT GAAGGGGTGG ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu14254
    Clone ID Related Accession (Same CDS sequence) XM_024451136.1 , XM_024451137.1 , NM_001144971.2 , NM_001144970.3 , NM_001144971.1 , NM_001144970.2 , XM_024451135.1 , XM_024451131.1
    Accession Version XM_024451137.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2505bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGAGCGAC CCTATACATT TAAGGACTTT CTCCTCAGAC CAAGAAGTCA TAAGTCTCGA 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCAA AAAATGGAGG TCAAGATGAA
    GAAAACAGTG ACCAGAGGGA TGACATGGAG CATGGATGGG AAGTTGTTGA CTCAAATGAC
    TCGGCTTCTC AGCACCAAGA GGAACTTCCT CCTCCTCCTC TGCCTCCCGG GTGGGAAGAA
    AAAGTGGACA ATTTAGGCCG AACTTACTAT GTCAACCACA ACAACCGGAC CACTCAGTGG
    CACAGACCAA GCCTGATGGA CGTGTCCTCG GAGTCGGACA ATAACATCAG ACAGATCAAC
    CAGGAGGCAG CACACCGGCG CTTCCGCTCC CGCAGGCACA TCAGCGAAGA CTTGGAGCCC
    GAGCCCTCGG AGGGCGGGGA TGTCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAT
    ATCGCTGGAG ACTCTCTCGG TCTGGCTCTG CCCCCACCAC CGGCCTCCCC AGGATCTCGG
    ACCAGCCCTC AGGAGCTGTC AGAGGAACTA AGCAGAAGGC TTCAGATCAC TCCAGACTCC
    AATGGGGAAC AGTTCAGCTC TTTGATTCAA AGAGAACCCT CCTCAAGGTT GAGGTCATGC
    AGTGTCACCG ACGCAGTTGC AGAACAGGGC CATCTACCAC CGCCATCAGT GGCCTATGTA
    CATACCACGC CGGGTCTGCC TTCAGGCTGG GAAGAAAGAA AAGATGCTAA GGGGCGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GACCTATCAT GCAGCTTGCA
    GAAGATGGTG CGTCCGGATC AGCCACAAAC AGTAACAACC ATCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CTGCCCCGCT GGAGGGTGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GACACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CTTGCCACCC
    GGCTGGGAAA TGAGGATAGC GCCAAACGGC CGGCCCTTCT TCATTGATCA TAACACAAAG
    ACTACAACCT GGGAAGATCC ACGTTTGAAA TTTCCAGTAC ATATGCGGTC AAAGACATCT
    TTAAACCCCA ATGACCTTGG CCCCCTTCCT CCTGGCTGGG AAGAAAGAAT TCACTTGGAT
    GGCCGAACGT TTTATATTGA TCATAATAGC AAAATTACTC AGTGGGAAGA CCCAAGACTG
    CAGAACCCAG CTATTACTGG TCCGGCTGTC CCTTACTCCA GAGAATTTAA GCAGAAATAT
    GACTACTTCA GGAAGAAATT AAAGAAACCT GCTGATATCC CCAATAGGTT TGAAATGAAA
    CTTCACAGAA ATAACATATT TGAAGAGTCC TATCGGAGAA TTATGTCCGT GAAAAGACCA
    GATGTCCTAA AAGCTAGACT GTGGATTGAG TTTGAATCAG AGAAAGGTCT TGACTATGGG
    GGTGTGGCCA GAGAATGGTT CTTCTTACTG TCCAAAGAGA TGTTCAACCC CTACTACGGC
    CTCTTTGAGT ACTCTGCCAC GGACAACTAC ACCCTTCAGA TCAACCCTAA TTCAGGCCTC
    TGTAATGAGG ATCATTTGTC CTACTTCACT TTTATTGGAA GAGTTGCTGG TCTGGCCGTA
    TTTCATGGGA AGCTCTTAGA TGGTTTCTTC ATTAGACCAT TTTACAAGAT GATGTTGGGA
    AAGCAGATAA CCCTGAATGA CATGGAATCT GTGGATAGTG AATATTACAA CTCTTTGAAA
    TGGATCCTGG AGAATGACCC TACTGAGCTG GACCTCATGT TCTGCATAGA CGAAGAAAAC
    TTTGGACAGA CATATCAAGT GGATTTGAAG CCCAATGGGT CAGAAATAAT GGTCACAAAT
    GAAAACAAAA GGGAATATAT CGACTTAGTC ATCCAGTGGA GATTTGTGAA CAGGGTCCAG
    AAGCAGATGA ACGCCTTCTT GGAGGGATTC ACAGAACTAC TTCCTATTGA TTTGATTAAA
    ATTTTTGATG AAAATGAGCT GGAGTTGCTC ATGTGCGGCC TCGGTGATGT GGATGTGAAT
    GACTGGAGAC AGCATTCTAT TTACAAGAAC GGCTACTGCC CAAACCACCC CGTCATTCAG
    TGGTTCTGGA AGGCTGTGCT ACTCATGGAC GCCGAAAAGC GTATCCGGTT ACTGCAGTTT
    GTCACAGGGA CATCGCGAGT ACCTATGAAT GGATTTGCCG AACTTTATGG TTCCAATGGT
    CCTCAGCTGT TTACAATAGA GCAATGGGGC AGTCCTGAGA AACTGCCCAG AGCTCACACA
    TGCTTTAATC GCCTTGACTT ACCTCCATAT GAAACCTTTG AAGATTTACG AGAGAAACTT
    CTCATGGCCG TGGAAAATGC TCAAGGATTT GAAGGGGTGG ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024306905.1
    CDS1335..3839
    Translation

    Target ORF information:

    RefSeq Version XM_024451137.1
    Organism Homo sapiens(human)
    Definition Homo sapiens NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant X23, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024451137.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGAGCGAC CCTATACATT TAAGGACTTT CTCCTCAGAC CAAGAAGTCA TAAGTCTCGA 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCAA AAAATGGAGG TCAAGATGAA
    GAAAACAGTG ACCAGAGGGA TGACATGGAG CATGGATGGG AAGTTGTTGA CTCAAATGAC
    TCGGCTTCTC AGCACCAAGA GGAACTTCCT CCTCCTCCTC TGCCTCCCGG GTGGGAAGAA
    AAAGTGGACA ATTTAGGCCG AACTTACTAT GTCAACCACA ACAACCGGAC CACTCAGTGG
    CACAGACCAA GCCTGATGGA CGTGTCCTCG GAGTCGGACA ATAACATCAG ACAGATCAAC
    CAGGAGGCAG CACACCGGCG CTTCCGCTCC CGCAGGCACA TCAGCGAAGA CTTGGAGCCC
    GAGCCCTCGG AGGGCGGGGA TGTCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAT
    ATCGCTGGAG ACTCTCTCGG TCTGGCTCTG CCCCCACCAC CGGCCTCCCC AGGATCTCGG
    ACCAGCCCTC AGGAGCTGTC AGAGGAACTA AGCAGAAGGC TTCAGATCAC TCCAGACTCC
    AATGGGGAAC AGTTCAGCTC TTTGATTCAA AGAGAACCCT CCTCAAGGTT GAGGTCATGC
    AGTGTCACCG ACGCAGTTGC AGAACAGGGC CATCTACCAC CGCCATCAGT GGCCTATGTA
    CATACCACGC CGGGTCTGCC TTCAGGCTGG GAAGAAAGAA AAGATGCTAA GGGGCGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GACCTATCAT GCAGCTTGCA
    GAAGATGGTG CGTCCGGATC AGCCACAAAC AGTAACAACC ATCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CTGCCCCGCT GGAGGGTGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GACACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CTTGCCACCC
    GGCTGGGAAA TGAGGATAGC GCCAAACGGC CGGCCCTTCT TCATTGATCA TAACACAAAG
    ACTACAACCT GGGAAGATCC ACGTTTGAAA TTTCCAGTAC ATATGCGGTC AAAGACATCT
    TTAAACCCCA ATGACCTTGG CCCCCTTCCT CCTGGCTGGG AAGAAAGAAT TCACTTGGAT
    GGCCGAACGT TTTATATTGA TCATAATAGC AAAATTACTC AGTGGGAAGA CCCAAGACTG
    CAGAACCCAG CTATTACTGG TCCGGCTGTC CCTTACTCCA GAGAATTTAA GCAGAAATAT
    GACTACTTCA GGAAGAAATT AAAGAAACCT GCTGATATCC CCAATAGGTT TGAAATGAAA
    CTTCACAGAA ATAACATATT TGAAGAGTCC TATCGGAGAA TTATGTCCGT GAAAAGACCA
    GATGTCCTAA AAGCTAGACT GTGGATTGAG TTTGAATCAG AGAAAGGTCT TGACTATGGG
    GGTGTGGCCA GAGAATGGTT CTTCTTACTG TCCAAAGAGA TGTTCAACCC CTACTACGGC
    CTCTTTGAGT ACTCTGCCAC GGACAACTAC ACCCTTCAGA TCAACCCTAA TTCAGGCCTC
    TGTAATGAGG ATCATTTGTC CTACTTCACT TTTATTGGAA GAGTTGCTGG TCTGGCCGTA
    TTTCATGGGA AGCTCTTAGA TGGTTTCTTC ATTAGACCAT TTTACAAGAT GATGTTGGGA
    AAGCAGATAA CCCTGAATGA CATGGAATCT GTGGATAGTG AATATTACAA CTCTTTGAAA
    TGGATCCTGG AGAATGACCC TACTGAGCTG GACCTCATGT TCTGCATAGA CGAAGAAAAC
    TTTGGACAGA CATATCAAGT GGATTTGAAG CCCAATGGGT CAGAAATAAT GGTCACAAAT
    GAAAACAAAA GGGAATATAT CGACTTAGTC ATCCAGTGGA GATTTGTGAA CAGGGTCCAG
    AAGCAGATGA ACGCCTTCTT GGAGGGATTC ACAGAACTAC TTCCTATTGA TTTGATTAAA
    ATTTTTGATG AAAATGAGCT GGAGTTGCTC ATGTGCGGCC TCGGTGATGT GGATGTGAAT
    GACTGGAGAC AGCATTCTAT TTACAAGAAC GGCTACTGCC CAAACCACCC CGTCATTCAG
    TGGTTCTGGA AGGCTGTGCT ACTCATGGAC GCCGAAAAGC GTATCCGGTT ACTGCAGTTT
    GTCACAGGGA CATCGCGAGT ACCTATGAAT GGATTTGCCG AACTTTATGG TTCCAATGGT
    CCTCAGCTGT TTACAATAGA GCAATGGGGC AGTCCTGAGA AACTGCCCAG AGCTCACACA
    TGCTTTAATC GCCTTGACTT ACCTCCATAT GAAACCTTTG AAGATTTACG AGAGAAACTT
    CTCATGGCCG TGGAAAATGC TCAAGGATTT GAAGGGGTGG ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu14254
    Clone ID Related Accession (Same CDS sequence) XM_024451136.1 , XM_024451137.1 , NM_001144971.2 , NM_001144970.3 , NM_001144971.1 , NM_001144970.2 , XM_024451135.1 , XM_024451131.1
    Accession Version XM_024451135.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2505bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGAGCGAC CCTATACATT TAAGGACTTT CTCCTCAGAC CAAGAAGTCA TAAGTCTCGA 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCAA AAAATGGAGG TCAAGATGAA
    GAAAACAGTG ACCAGAGGGA TGACATGGAG CATGGATGGG AAGTTGTTGA CTCAAATGAC
    TCGGCTTCTC AGCACCAAGA GGAACTTCCT CCTCCTCCTC TGCCTCCCGG GTGGGAAGAA
    AAAGTGGACA ATTTAGGCCG AACTTACTAT GTCAACCACA ACAACCGGAC CACTCAGTGG
    CACAGACCAA GCCTGATGGA CGTGTCCTCG GAGTCGGACA ATAACATCAG ACAGATCAAC
    CAGGAGGCAG CACACCGGCG CTTCCGCTCC CGCAGGCACA TCAGCGAAGA CTTGGAGCCC
    GAGCCCTCGG AGGGCGGGGA TGTCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAT
    ATCGCTGGAG ACTCTCTCGG TCTGGCTCTG CCCCCACCAC CGGCCTCCCC AGGATCTCGG
    ACCAGCCCTC AGGAGCTGTC AGAGGAACTA AGCAGAAGGC TTCAGATCAC TCCAGACTCC
    AATGGGGAAC AGTTCAGCTC TTTGATTCAA AGAGAACCCT CCTCAAGGTT GAGGTCATGC
    AGTGTCACCG ACGCAGTTGC AGAACAGGGC CATCTACCAC CGCCATCAGT GGCCTATGTA
    CATACCACGC CGGGTCTGCC TTCAGGCTGG GAAGAAAGAA AAGATGCTAA GGGGCGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GACCTATCAT GCAGCTTGCA
    GAAGATGGTG CGTCCGGATC AGCCACAAAC AGTAACAACC ATCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CTGCCCCGCT GGAGGGTGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GACACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CTTGCCACCC
    GGCTGGGAAA TGAGGATAGC GCCAAACGGC CGGCCCTTCT TCATTGATCA TAACACAAAG
    ACTACAACCT GGGAAGATCC ACGTTTGAAA TTTCCAGTAC ATATGCGGTC AAAGACATCT
    TTAAACCCCA ATGACCTTGG CCCCCTTCCT CCTGGCTGGG AAGAAAGAAT TCACTTGGAT
    GGCCGAACGT TTTATATTGA TCATAATAGC AAAATTACTC AGTGGGAAGA CCCAAGACTG
    CAGAACCCAG CTATTACTGG TCCGGCTGTC CCTTACTCCA GAGAATTTAA GCAGAAATAT
    GACTACTTCA GGAAGAAATT AAAGAAACCT GCTGATATCC CCAATAGGTT TGAAATGAAA
    CTTCACAGAA ATAACATATT TGAAGAGTCC TATCGGAGAA TTATGTCCGT GAAAAGACCA
    GATGTCCTAA AAGCTAGACT GTGGATTGAG TTTGAATCAG AGAAAGGTCT TGACTATGGG
    GGTGTGGCCA GAGAATGGTT CTTCTTACTG TCCAAAGAGA TGTTCAACCC CTACTACGGC
    CTCTTTGAGT ACTCTGCCAC GGACAACTAC ACCCTTCAGA TCAACCCTAA TTCAGGCCTC
    TGTAATGAGG ATCATTTGTC CTACTTCACT TTTATTGGAA GAGTTGCTGG TCTGGCCGTA
    TTTCATGGGA AGCTCTTAGA TGGTTTCTTC ATTAGACCAT TTTACAAGAT GATGTTGGGA
    AAGCAGATAA CCCTGAATGA CATGGAATCT GTGGATAGTG AATATTACAA CTCTTTGAAA
    TGGATCCTGG AGAATGACCC TACTGAGCTG GACCTCATGT TCTGCATAGA CGAAGAAAAC
    TTTGGACAGA CATATCAAGT GGATTTGAAG CCCAATGGGT CAGAAATAAT GGTCACAAAT
    GAAAACAAAA GGGAATATAT CGACTTAGTC ATCCAGTGGA GATTTGTGAA CAGGGTCCAG
    AAGCAGATGA ACGCCTTCTT GGAGGGATTC ACAGAACTAC TTCCTATTGA TTTGATTAAA
    ATTTTTGATG AAAATGAGCT GGAGTTGCTC ATGTGCGGCC TCGGTGATGT GGATGTGAAT
    GACTGGAGAC AGCATTCTAT TTACAAGAAC GGCTACTGCC CAAACCACCC CGTCATTCAG
    TGGTTCTGGA AGGCTGTGCT ACTCATGGAC GCCGAAAAGC GTATCCGGTT ACTGCAGTTT
    GTCACAGGGA CATCGCGAGT ACCTATGAAT GGATTTGCCG AACTTTATGG TTCCAATGGT
    CCTCAGCTGT TTACAATAGA GCAATGGGGC AGTCCTGAGA AACTGCCCAG AGCTCACACA
    TGCTTTAATC GCCTTGACTT ACCTCCATAT GAAACCTTTG AAGATTTACG AGAGAAACTT
    CTCATGGCCG TGGAAAATGC TCAAGGATTT GAAGGGGTGG ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024306903.1
    CDS423..2927
    Translation

    Target ORF information:

    RefSeq Version XM_024451135.1
    Organism Homo sapiens(human)
    Definition Homo sapiens NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant X21, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024451135.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGAGCGAC CCTATACATT TAAGGACTTT CTCCTCAGAC CAAGAAGTCA TAAGTCTCGA 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCAA AAAATGGAGG TCAAGATGAA
    GAAAACAGTG ACCAGAGGGA TGACATGGAG CATGGATGGG AAGTTGTTGA CTCAAATGAC
    TCGGCTTCTC AGCACCAAGA GGAACTTCCT CCTCCTCCTC TGCCTCCCGG GTGGGAAGAA
    AAAGTGGACA ATTTAGGCCG AACTTACTAT GTCAACCACA ACAACCGGAC CACTCAGTGG
    CACAGACCAA GCCTGATGGA CGTGTCCTCG GAGTCGGACA ATAACATCAG ACAGATCAAC
    CAGGAGGCAG CACACCGGCG CTTCCGCTCC CGCAGGCACA TCAGCGAAGA CTTGGAGCCC
    GAGCCCTCGG AGGGCGGGGA TGTCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAT
    ATCGCTGGAG ACTCTCTCGG TCTGGCTCTG CCCCCACCAC CGGCCTCCCC AGGATCTCGG
    ACCAGCCCTC AGGAGCTGTC AGAGGAACTA AGCAGAAGGC TTCAGATCAC TCCAGACTCC
    AATGGGGAAC AGTTCAGCTC TTTGATTCAA AGAGAACCCT CCTCAAGGTT GAGGTCATGC
    AGTGTCACCG ACGCAGTTGC AGAACAGGGC CATCTACCAC CGCCATCAGT GGCCTATGTA
    CATACCACGC CGGGTCTGCC TTCAGGCTGG GAAGAAAGAA AAGATGCTAA GGGGCGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GACCTATCAT GCAGCTTGCA
    GAAGATGGTG CGTCCGGATC AGCCACAAAC AGTAACAACC ATCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CTGCCCCGCT GGAGGGTGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GACACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CTTGCCACCC
    GGCTGGGAAA TGAGGATAGC GCCAAACGGC CGGCCCTTCT TCATTGATCA TAACACAAAG
    ACTACAACCT GGGAAGATCC ACGTTTGAAA TTTCCAGTAC ATATGCGGTC AAAGACATCT
    TTAAACCCCA ATGACCTTGG CCCCCTTCCT CCTGGCTGGG AAGAAAGAAT TCACTTGGAT
    GGCCGAACGT TTTATATTGA TCATAATAGC AAAATTACTC AGTGGGAAGA CCCAAGACTG
    CAGAACCCAG CTATTACTGG TCCGGCTGTC CCTTACTCCA GAGAATTTAA GCAGAAATAT
    GACTACTTCA GGAAGAAATT AAAGAAACCT GCTGATATCC CCAATAGGTT TGAAATGAAA
    CTTCACAGAA ATAACATATT TGAAGAGTCC TATCGGAGAA TTATGTCCGT GAAAAGACCA
    GATGTCCTAA AAGCTAGACT GTGGATTGAG TTTGAATCAG AGAAAGGTCT TGACTATGGG
    GGTGTGGCCA GAGAATGGTT CTTCTTACTG TCCAAAGAGA TGTTCAACCC CTACTACGGC
    CTCTTTGAGT ACTCTGCCAC GGACAACTAC ACCCTTCAGA TCAACCCTAA TTCAGGCCTC
    TGTAATGAGG ATCATTTGTC CTACTTCACT TTTATTGGAA GAGTTGCTGG TCTGGCCGTA
    TTTCATGGGA AGCTCTTAGA TGGTTTCTTC ATTAGACCAT TTTACAAGAT GATGTTGGGA
    AAGCAGATAA CCCTGAATGA CATGGAATCT GTGGATAGTG AATATTACAA CTCTTTGAAA
    TGGATCCTGG AGAATGACCC TACTGAGCTG GACCTCATGT TCTGCATAGA CGAAGAAAAC
    TTTGGACAGA CATATCAAGT GGATTTGAAG CCCAATGGGT CAGAAATAAT GGTCACAAAT
    GAAAACAAAA GGGAATATAT CGACTTAGTC ATCCAGTGGA GATTTGTGAA CAGGGTCCAG
    AAGCAGATGA ACGCCTTCTT GGAGGGATTC ACAGAACTAC TTCCTATTGA TTTGATTAAA
    ATTTTTGATG AAAATGAGCT GGAGTTGCTC ATGTGCGGCC TCGGTGATGT GGATGTGAAT
    GACTGGAGAC AGCATTCTAT TTACAAGAAC GGCTACTGCC CAAACCACCC CGTCATTCAG
    TGGTTCTGGA AGGCTGTGCT ACTCATGGAC GCCGAAAAGC GTATCCGGTT ACTGCAGTTT
    GTCACAGGGA CATCGCGAGT ACCTATGAAT GGATTTGCCG AACTTTATGG TTCCAATGGT
    CCTCAGCTGT TTACAATAGA GCAATGGGGC AGTCCTGAGA AACTGCCCAG AGCTCACACA
    TGCTTTAATC GCCTTGACTT ACCTCCATAT GAAACCTTTG AAGATTTACG AGAGAAACTT
    CTCATGGCCG TGGAAAATGC TCAAGGATTT GAAGGGGTGG ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu14254
    Clone ID Related Accession (Same CDS sequence) XM_024451136.1 , XM_024451137.1 , NM_001144971.2 , NM_001144970.3 , NM_001144971.1 , NM_001144970.2 , XM_024451135.1 , XM_024451131.1
    Accession Version XM_024451131.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2505bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGAGCGAC CCTATACATT TAAGGACTTT CTCCTCAGAC CAAGAAGTCA TAAGTCTCGA 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCAA AAAATGGAGG TCAAGATGAA
    GAAAACAGTG ACCAGAGGGA TGACATGGAG CATGGATGGG AAGTTGTTGA CTCAAATGAC
    TCGGCTTCTC AGCACCAAGA GGAACTTCCT CCTCCTCCTC TGCCTCCCGG GTGGGAAGAA
    AAAGTGGACA ATTTAGGCCG AACTTACTAT GTCAACCACA ACAACCGGAC CACTCAGTGG
    CACAGACCAA GCCTGATGGA CGTGTCCTCG GAGTCGGACA ATAACATCAG ACAGATCAAC
    CAGGAGGCAG CACACCGGCG CTTCCGCTCC CGCAGGCACA TCAGCGAAGA CTTGGAGCCC
    GAGCCCTCGG AGGGCGGGGA TGTCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAT
    ATCGCTGGAG ACTCTCTCGG TCTGGCTCTG CCCCCACCAC CGGCCTCCCC AGGATCTCGG
    ACCAGCCCTC AGGAGCTGTC AGAGGAACTA AGCAGAAGGC TTCAGATCAC TCCAGACTCC
    AATGGGGAAC AGTTCAGCTC TTTGATTCAA AGAGAACCCT CCTCAAGGTT GAGGTCATGC
    AGTGTCACCG ACGCAGTTGC AGAACAGGGC CATCTACCAC CGCCATCAGT GGCCTATGTA
    CATACCACGC CGGGTCTGCC TTCAGGCTGG GAAGAAAGAA AAGATGCTAA GGGGCGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GACCTATCAT GCAGCTTGCA
    GAAGATGGTG CGTCCGGATC AGCCACAAAC AGTAACAACC ATCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CTGCCCCGCT GGAGGGTGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GACACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CTTGCCACCC
    GGCTGGGAAA TGAGGATAGC GCCAAACGGC CGGCCCTTCT TCATTGATCA TAACACAAAG
    ACTACAACCT GGGAAGATCC ACGTTTGAAA TTTCCAGTAC ATATGCGGTC AAAGACATCT
    TTAAACCCCA ATGACCTTGG CCCCCTTCCT CCTGGCTGGG AAGAAAGAAT TCACTTGGAT
    GGCCGAACGT TTTATATTGA TCATAATAGC AAAATTACTC AGTGGGAAGA CCCAAGACTG
    CAGAACCCAG CTATTACTGG TCCGGCTGTC CCTTACTCCA GAGAATTTAA GCAGAAATAT
    GACTACTTCA GGAAGAAATT AAAGAAACCT GCTGATATCC CCAATAGGTT TGAAATGAAA
    CTTCACAGAA ATAACATATT TGAAGAGTCC TATCGGAGAA TTATGTCCGT GAAAAGACCA
    GATGTCCTAA AAGCTAGACT GTGGATTGAG TTTGAATCAG AGAAAGGTCT TGACTATGGG
    GGTGTGGCCA GAGAATGGTT CTTCTTACTG TCCAAAGAGA TGTTCAACCC CTACTACGGC
    CTCTTTGAGT ACTCTGCCAC GGACAACTAC ACCCTTCAGA TCAACCCTAA TTCAGGCCTC
    TGTAATGAGG ATCATTTGTC CTACTTCACT TTTATTGGAA GAGTTGCTGG TCTGGCCGTA
    TTTCATGGGA AGCTCTTAGA TGGTTTCTTC ATTAGACCAT TTTACAAGAT GATGTTGGGA
    AAGCAGATAA CCCTGAATGA CATGGAATCT GTGGATAGTG AATATTACAA CTCTTTGAAA
    TGGATCCTGG AGAATGACCC TACTGAGCTG GACCTCATGT TCTGCATAGA CGAAGAAAAC
    TTTGGACAGA CATATCAAGT GGATTTGAAG CCCAATGGGT CAGAAATAAT GGTCACAAAT
    GAAAACAAAA GGGAATATAT CGACTTAGTC ATCCAGTGGA GATTTGTGAA CAGGGTCCAG
    AAGCAGATGA ACGCCTTCTT GGAGGGATTC ACAGAACTAC TTCCTATTGA TTTGATTAAA
    ATTTTTGATG AAAATGAGCT GGAGTTGCTC ATGTGCGGCC TCGGTGATGT GGATGTGAAT
    GACTGGAGAC AGCATTCTAT TTACAAGAAC GGCTACTGCC CAAACCACCC CGTCATTCAG
    TGGTTCTGGA AGGCTGTGCT ACTCATGGAC GCCGAAAAGC GTATCCGGTT ACTGCAGTTT
    GTCACAGGGA CATCGCGAGT ACCTATGAAT GGATTTGCCG AACTTTATGG TTCCAATGGT
    CCTCAGCTGT TTACAATAGA GCAATGGGGC AGTCCTGAGA AACTGCCCAG AGCTCACACA
    TGCTTTAATC GCCTTGACTT ACCTCCATAT GAAACCTTTG AAGATTTACG AGAGAAACTT
    CTCATGGCCG TGGAAAATGC TCAAGGATTT GAAGGGGTGG ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024306899.1
    CDS489..2993
    Translation

    Target ORF information:

    RefSeq Version XM_024451131.1
    Organism Homo sapiens(human)
    Definition Homo sapiens NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024451131.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGAGCGAC CCTATACATT TAAGGACTTT CTCCTCAGAC CAAGAAGTCA TAAGTCTCGA 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCAA AAAATGGAGG TCAAGATGAA
    GAAAACAGTG ACCAGAGGGA TGACATGGAG CATGGATGGG AAGTTGTTGA CTCAAATGAC
    TCGGCTTCTC AGCACCAAGA GGAACTTCCT CCTCCTCCTC TGCCTCCCGG GTGGGAAGAA
    AAAGTGGACA ATTTAGGCCG AACTTACTAT GTCAACCACA ACAACCGGAC CACTCAGTGG
    CACAGACCAA GCCTGATGGA CGTGTCCTCG GAGTCGGACA ATAACATCAG ACAGATCAAC
    CAGGAGGCAG CACACCGGCG CTTCCGCTCC CGCAGGCACA TCAGCGAAGA CTTGGAGCCC
    GAGCCCTCGG AGGGCGGGGA TGTCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAT
    ATCGCTGGAG ACTCTCTCGG TCTGGCTCTG CCCCCACCAC CGGCCTCCCC AGGATCTCGG
    ACCAGCCCTC AGGAGCTGTC AGAGGAACTA AGCAGAAGGC TTCAGATCAC TCCAGACTCC
    AATGGGGAAC AGTTCAGCTC TTTGATTCAA AGAGAACCCT CCTCAAGGTT GAGGTCATGC
    AGTGTCACCG ACGCAGTTGC AGAACAGGGC CATCTACCAC CGCCATCAGT GGCCTATGTA
    CATACCACGC CGGGTCTGCC TTCAGGCTGG GAAGAAAGAA AAGATGCTAA GGGGCGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GACCTATCAT GCAGCTTGCA
    GAAGATGGTG CGTCCGGATC AGCCACAAAC AGTAACAACC ATCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CTGCCCCGCT GGAGGGTGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GACACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CTTGCCACCC
    GGCTGGGAAA TGAGGATAGC GCCAAACGGC CGGCCCTTCT TCATTGATCA TAACACAAAG
    ACTACAACCT GGGAAGATCC ACGTTTGAAA TTTCCAGTAC ATATGCGGTC AAAGACATCT
    TTAAACCCCA ATGACCTTGG CCCCCTTCCT CCTGGCTGGG AAGAAAGAAT TCACTTGGAT
    GGCCGAACGT TTTATATTGA TCATAATAGC AAAATTACTC AGTGGGAAGA CCCAAGACTG
    CAGAACCCAG CTATTACTGG TCCGGCTGTC CCTTACTCCA GAGAATTTAA GCAGAAATAT
    GACTACTTCA GGAAGAAATT AAAGAAACCT GCTGATATCC CCAATAGGTT TGAAATGAAA
    CTTCACAGAA ATAACATATT TGAAGAGTCC TATCGGAGAA TTATGTCCGT GAAAAGACCA
    GATGTCCTAA AAGCTAGACT GTGGATTGAG TTTGAATCAG AGAAAGGTCT TGACTATGGG
    GGTGTGGCCA GAGAATGGTT CTTCTTACTG TCCAAAGAGA TGTTCAACCC CTACTACGGC
    CTCTTTGAGT ACTCTGCCAC GGACAACTAC ACCCTTCAGA TCAACCCTAA TTCAGGCCTC
    TGTAATGAGG ATCATTTGTC CTACTTCACT TTTATTGGAA GAGTTGCTGG TCTGGCCGTA
    TTTCATGGGA AGCTCTTAGA TGGTTTCTTC ATTAGACCAT TTTACAAGAT GATGTTGGGA
    AAGCAGATAA CCCTGAATGA CATGGAATCT GTGGATAGTG AATATTACAA CTCTTTGAAA
    TGGATCCTGG AGAATGACCC TACTGAGCTG GACCTCATGT TCTGCATAGA CGAAGAAAAC
    TTTGGACAGA CATATCAAGT GGATTTGAAG CCCAATGGGT CAGAAATAAT GGTCACAAAT
    GAAAACAAAA GGGAATATAT CGACTTAGTC ATCCAGTGGA GATTTGTGAA CAGGGTCCAG
    AAGCAGATGA ACGCCTTCTT GGAGGGATTC ACAGAACTAC TTCCTATTGA TTTGATTAAA
    ATTTTTGATG AAAATGAGCT GGAGTTGCTC ATGTGCGGCC TCGGTGATGT GGATGTGAAT
    GACTGGAGAC AGCATTCTAT TTACAAGAAC GGCTACTGCC CAAACCACCC CGTCATTCAG
    TGGTTCTGGA AGGCTGTGCT ACTCATGGAC GCCGAAAAGC GTATCCGGTT ACTGCAGTTT
    GTCACAGGGA CATCGCGAGT ACCTATGAAT GGATTTGCCG AACTTTATGG TTCCAATGGT
    CCTCAGCTGT TTACAATAGA GCAATGGGGC AGTCCTGAGA AACTGCCCAG AGCTCACACA
    TGCTTTAATC GCCTTGACTT ACCTCCATAT GAAACCTTTG AAGATTTACG AGAGAAACTT
    CTCATGGCCG TGGAAAATGC TCAAGGATTT GAAGGGGTGG ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu51146
    Clone ID Related Accession (Same CDS sequence) XM_006722428.3 , XM_006722428.4
    Accession Version XM_006722428.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2982bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006722428.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCGACCG GGCTCGGGGA GCCGGTCTAT GGACTTTCCG AAGACGAGGG AGAGTCCCGT 
    ATTCTCAGAG TAAAAGTTGT TTCTGGAATT GATCTCGCCA AAAAGGACAT CTTTGGAGCC
    AGTGATCCGT ATGTGAAACT TTCATTGTAC GTAGCGGATG AGAATAGAGA ACTTGCTTTG
    GTCCAGACAA AAACAATTAA AAAGACACTG AACCCAAAAT GGAATGAAGA ATTTTATTTC
    AGGGTAAACC CATCTAATCA CAGACTCCTA TTTGAAGTAT TTGACGAAAA TAGACTGACA
    CGAGACGACT TCCTGGGCCA GGTGGACGTG CCCCTTAGTC ACCTTCCGAC AGAAGATCCA
    ACCATGGAGC GACCCTATAC ATTTAAGGAC TTTCTCCTCA GACCAAGAAG TCATAAGTCT
    CGAGTTAAGG GATTTTTGCG ATTGAAAATG GCCTATATGC CAAAAAATGG AGGTCAAGAT
    GAAGAAAACA GTGACCAGAG GGATGACATG GAGCATGGAT GGGAAGTTGT TGACTCAAAT
    GACTCGGCTT CTCAGCACCA AGAGGAACTT CCTCCTCCTC CTCTGCCTCC CGGGTGGGAA
    GAAAAAGTGG ACAATTTAGG CCGAACTTAC TATGTCAACC ACAACAACCG GACCACTCAG
    TGGCACAGAC CAAGCCTGAT GGACGTGTCC TCGGAGTCGG ACAATAACAT CAGACAGATC
    AACCAGGAGG CAGCACACCG GCGCTTCCGC TCCCGCAGGC ACATCAGCGA AGACTTGGAG
    CCCGAGCCCT CGGAGGGCGG GGATGTCCCC GAGCCTTGGG AGACCATTTC AGAGGAAGTG
    AATATCGCTG GAGACTCTCT CGGTCTGGCT CTGCCCCCAC CACCGGCCTC CCCAGGATCT
    CGGACCAGCC CTCAGGAGCT GTCAGAGGAA CTAAGCAGAA GGCTTCAGAT CACTCCAGAC
    TCCAATGGGG AACAGTTCAG CTCTTTGATT CAAAGAGAAC CCTCCTCAAG GTTGAGGTCA
    TGCAGTGTCA CCGACGCAGT TGCAGAACAG GGCCATCTAC CACCGCCCAG TGCCCCAGCT
    GGGAGAGCGC GTTCATCAAC TGTCACGGGT GGTGAGGAAC CAACGCCATC AGTGGCCTAT
    GTACATACCA CGCCGGGTCT GCCTTCAGGC TGGGAAGAAA GAAAAGATGC TAAGGGGCGC
    ACATACTATG TCAATCATAA CAATCGAACC ACAACTTGGA CTCGACCTAT CATGCAGCTT
    GCAGAAGATG GTGCGTCCGG ATCAGCCACA AACAGTAACA ACCATCTAAT CGAGCCTCAG
    ATCCGCCGGC CTCGTAGCCT CAGCTCGCCA ACAGTAACTT TATCTGCCCC GCTGGAGGGT
    GCCAAGGACT CACCCGTACG TCGGGCTGTG AAAGACACCC TTTCCAACCC ACAGTCCCCA
    CAGCCATCAC CTTACAACTC CCCCAAACCA CAACACAAAG TCACACAGAG CTTCTTGCCA
    CCCGGCTGGG AAATGAGGAT AGCGCCAAAC GGCCGGCCCT TCTTCATTGA TCATAACACA
    AAGACTACAA CCTGGGAAGA TCCACGTTTG AAATTTCCAG TACATATGCG GTCAAAGACA
    TCTTTAAACC CCAATGACCT TGGCCCCCTT CCTCCTGGCT GGGAAGAAAG AATTCACTTG
    GATGGCCGAA CGTTTTATAT TGATCATAGC AGCCAGGTGT TATGTGGAGA AAATGATACC
    AGAGAATCAG TGCCTTCATA CGATAGCAAA ATTACTCAGT GGGAAGACCC AAGACTGCAG
    AACCCAGCTA TTACTGGTCC GGCTGTCCCT TACTCCAGAG AATTTAAGCA GAAATATGAC
    TACTTCAGGA AGAAATTAAA GAAACCTGCT GATATCCCCA ATAGGTTTGA AATGAAACTT
    CACAGAAATA ACATATTTGA AGAGTCCTAT CGGAGAATTA TGTCCGTGAA AAGACCAGAT
    GTCCTAAAAG CTAGACTGTG GATTGAGTTT GAATCAGAGA AAGGTCTTGA CTATGGGGGT
    GTGGCCAGAG AATGGTTCTT CTTACTGTCC AAAGAGATGT TCAACCCCTA CTACGGCCTC
    TTTGAGTACT CTGCCACGGA CAACTACACC CTTCAGATCA ACCCTAATTC AGGCCTCTGT
    AATGAGGATC ATTTGTCCTA CTTCACTTTT ATTGGAAGAG TTGCTGGTCT GGCCGTATTT
    CATGGGAAGC TCTTAGATGG TTTCTTCATT AGACCATTTT ACAAGATGAT GTTGGGAAAG
    CAGATAACCC TGAATGACAT GGAATCTGTG GATAGTGAAT ATTACAACTC TTTGAAATGG
    ATCCTGGAGA ATGACCCTAC TGAGCTGGAC CTCATGTTCT GCATAGACGA AGAAAACTTT
    GGACAGACAT ATCAAGTGGA TTTGAAGCCC AATGGGTCAG AAATAATGGT CACAAATGAA
    AACAAAAGGG AATATATCGA CTTAGTCATC CAGTGGAGAT TTGTGAACAG GGTCCAGAAG
    CAGATGAACG CCTTCTTGGA GGGATTCACA GAACTACTTC CTATTGATTT GATTAAAATT
    TTTGATGAAA ATGAGCTGGA GTTGCTCATG TGCGGCCTCG GTGATGTGGA TGTGAATGAC
    TGGAGACAGC ATTCTATTTA CAAGAACGGC TACTGCCCAA ACCACCCCGT CATTCAGTGG
    TTCTGGAAGG CTGTGCTACT CATGGACGCC GAAAAGCGTA TCCGGTTACT GCAGTTTGTC
    ACAGGGACAT CGCGAGTACC TATGAATGGA TTTGCCGAAC TTTATGGTTC CAATGGTCCT
    CAGCTGTTTA CAATAGAGCA ATGGGGCAGT CCTGAGAAAC TGCCCAGAGC TCACACATGC
    TTTAATCGCC TTGACTTACC TCCATATGAA ACCTTTGAAG ATTTACGAGA GAAACTTCTC
    ATGGCCGTGG AAAATGCTCA AGGATTTGAA GGGGTGGATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006722491.1
    CDS300..3281
    Translation

    Target ORF information:

    RefSeq Version XM_006722428.3
    Organism Homo sapiens(human)
    Definition Homo sapiens neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006722428.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCGACCG GGCTCGGGGA GCCGGTCTAT GGACTTTCCG AAGACGAGGG AGAGTCCCGT 
    ATTCTCAGAG TAAAAGTTGT TTCTGGAATT GATCTCGCCA AAAAGGACAT CTTTGGAGCC
    AGTGATCCGT ATGTGAAACT TTCATTGTAC GTAGCGGATG AGAATAGAGA ACTTGCTTTG
    GTCCAGACAA AAACAATTAA AAAGACACTG AACCCAAAAT GGAATGAAGA ATTTTATTTC
    AGGGTAAACC CATCTAATCA CAGACTCCTA TTTGAAGTAT TTGACGAAAA TAGACTGACA
    CGAGACGACT TCCTGGGCCA GGTGGACGTG CCCCTTAGTC ACCTTCCGAC AGAAGATCCA
    ACCATGGAGC GACCCTATAC ATTTAAGGAC TTTCTCCTCA GACCAAGAAG TCATAAGTCT
    CGAGTTAAGG GATTTTTGCG ATTGAAAATG GCCTATATGC CAAAAAATGG AGGTCAAGAT
    GAAGAAAACA GTGACCAGAG GGATGACATG GAGCATGGAT GGGAAGTTGT TGACTCAAAT
    GACTCGGCTT CTCAGCACCA AGAGGAACTT CCTCCTCCTC CTCTGCCTCC CGGGTGGGAA
    GAAAAAGTGG ACAATTTAGG CCGAACTTAC TATGTCAACC ACAACAACCG GACCACTCAG
    TGGCACAGAC CAAGCCTGAT GGACGTGTCC TCGGAGTCGG ACAATAACAT CAGACAGATC
    AACCAGGAGG CAGCACACCG GCGCTTCCGC TCCCGCAGGC ACATCAGCGA AGACTTGGAG
    CCCGAGCCCT CGGAGGGCGG GGATGTCCCC GAGCCTTGGG AGACCATTTC AGAGGAAGTG
    AATATCGCTG GAGACTCTCT CGGTCTGGCT CTGCCCCCAC CACCGGCCTC CCCAGGATCT
    CGGACCAGCC CTCAGGAGCT GTCAGAGGAA CTAAGCAGAA GGCTTCAGAT CACTCCAGAC
    TCCAATGGGG AACAGTTCAG CTCTTTGATT CAAAGAGAAC CCTCCTCAAG GTTGAGGTCA
    TGCAGTGTCA CCGACGCAGT TGCAGAACAG GGCCATCTAC CACCGCCCAG TGCCCCAGCT
    GGGAGAGCGC GTTCATCAAC TGTCACGGGT GGTGAGGAAC CAACGCCATC AGTGGCCTAT
    GTACATACCA CGCCGGGTCT GCCTTCAGGC TGGGAAGAAA GAAAAGATGC TAAGGGGCGC
    ACATACTATG TCAATCATAA CAATCGAACC ACAACTTGGA CTCGACCTAT CATGCAGCTT
    GCAGAAGATG GTGCGTCCGG ATCAGCCACA AACAGTAACA ACCATCTAAT CGAGCCTCAG
    ATCCGCCGGC CTCGTAGCCT CAGCTCGCCA ACAGTAACTT TATCTGCCCC GCTGGAGGGT
    GCCAAGGACT CACCCGTACG TCGGGCTGTG AAAGACACCC TTTCCAACCC ACAGTCCCCA
    CAGCCATCAC CTTACAACTC CCCCAAACCA CAACACAAAG TCACACAGAG CTTCTTGCCA
    CCCGGCTGGG AAATGAGGAT AGCGCCAAAC GGCCGGCCCT TCTTCATTGA TCATAACACA
    AAGACTACAA CCTGGGAAGA TCCACGTTTG AAATTTCCAG TACATATGCG GTCAAAGACA
    TCTTTAAACC CCAATGACCT TGGCCCCCTT CCTCCTGGCT GGGAAGAAAG AATTCACTTG
    GATGGCCGAA CGTTTTATAT TGATCATAGC AGCCAGGTGT TATGTGGAGA AAATGATACC
    AGAGAATCAG TGCCTTCATA CGATAGCAAA ATTACTCAGT GGGAAGACCC AAGACTGCAG
    AACCCAGCTA TTACTGGTCC GGCTGTCCCT TACTCCAGAG AATTTAAGCA GAAATATGAC
    TACTTCAGGA AGAAATTAAA GAAACCTGCT GATATCCCCA ATAGGTTTGA AATGAAACTT
    CACAGAAATA ACATATTTGA AGAGTCCTAT CGGAGAATTA TGTCCGTGAA AAGACCAGAT
    GTCCTAAAAG CTAGACTGTG GATTGAGTTT GAATCAGAGA AAGGTCTTGA CTATGGGGGT
    GTGGCCAGAG AATGGTTCTT CTTACTGTCC AAAGAGATGT TCAACCCCTA CTACGGCCTC
    TTTGAGTACT CTGCCACGGA CAACTACACC CTTCAGATCA ACCCTAATTC AGGCCTCTGT
    AATGAGGATC ATTTGTCCTA CTTCACTTTT ATTGGAAGAG TTGCTGGTCT GGCCGTATTT
    CATGGGAAGC TCTTAGATGG TTTCTTCATT AGACCATTTT ACAAGATGAT GTTGGGAAAG
    CAGATAACCC TGAATGACAT GGAATCTGTG GATAGTGAAT ATTACAACTC TTTGAAATGG
    ATCCTGGAGA ATGACCCTAC TGAGCTGGAC CTCATGTTCT GCATAGACGA AGAAAACTTT
    GGACAGACAT ATCAAGTGGA TTTGAAGCCC AATGGGTCAG AAATAATGGT CACAAATGAA
    AACAAAAGGG AATATATCGA CTTAGTCATC CAGTGGAGAT TTGTGAACAG GGTCCAGAAG
    CAGATGAACG CCTTCTTGGA GGGATTCACA GAACTACTTC CTATTGATTT GATTAAAATT
    TTTGATGAAA ATGAGCTGGA GTTGCTCATG TGCGGCCTCG GTGATGTGGA TGTGAATGAC
    TGGAGACAGC ATTCTATTTA CAAGAACGGC TACTGCCCAA ACCACCCCGT CATTCAGTGG
    TTCTGGAAGG CTGTGCTACT CATGGACGCC GAAAAGCGTA TCCGGTTACT GCAGTTTGTC
    ACAGGGACAT CGCGAGTACC TATGAATGGA TTTGCCGAAC TTTATGGTTC CAATGGTCCT
    CAGCTGTTTA CAATAGAGCA ATGGGGCAGT CCTGAGAAAC TGCCCAGAGC TCACACATGC
    TTTAATCGCC TTGACTTACC TCCATATGAA ACCTTTGAAG ATTTACGAGA GAAACTTCTC
    ATGGCCGTGG AAAATGCTCA AGGATTTGAA GGGGTGGATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu51146
    Clone ID Related Accession (Same CDS sequence) XM_006722428.3 , XM_006722428.4
    Accession Version XM_006722428.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2982bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_006722428.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCGACCG GGCTCGGGGA GCCGGTCTAT GGACTTTCCG AAGACGAGGG AGAGTCCCGT 
    ATTCTCAGAG TAAAAGTTGT TTCTGGAATT GATCTCGCCA AAAAGGACAT CTTTGGAGCC
    AGTGATCCGT ATGTGAAACT TTCATTGTAC GTAGCGGATG AGAATAGAGA ACTTGCTTTG
    GTCCAGACAA AAACAATTAA AAAGACACTG AACCCAAAAT GGAATGAAGA ATTTTATTTC
    AGGGTAAACC CATCTAATCA CAGACTCCTA TTTGAAGTAT TTGACGAAAA TAGACTGACA
    CGAGACGACT TCCTGGGCCA GGTGGACGTG CCCCTTAGTC ACCTTCCGAC AGAAGATCCA
    ACCATGGAGC GACCCTATAC ATTTAAGGAC TTTCTCCTCA GACCAAGAAG TCATAAGTCT
    CGAGTTAAGG GATTTTTGCG ATTGAAAATG GCCTATATGC CAAAAAATGG AGGTCAAGAT
    GAAGAAAACA GTGACCAGAG GGATGACATG GAGCATGGAT GGGAAGTTGT TGACTCAAAT
    GACTCGGCTT CTCAGCACCA AGAGGAACTT CCTCCTCCTC CTCTGCCTCC CGGGTGGGAA
    GAAAAAGTGG ACAATTTAGG CCGAACTTAC TATGTCAACC ACAACAACCG GACCACTCAG
    TGGCACAGAC CAAGCCTGAT GGACGTGTCC TCGGAGTCGG ACAATAACAT CAGACAGATC
    AACCAGGAGG CAGCACACCG GCGCTTCCGC TCCCGCAGGC ACATCAGCGA AGACTTGGAG
    CCCGAGCCCT CGGAGGGCGG GGATGTCCCC GAGCCTTGGG AGACCATTTC AGAGGAAGTG
    AATATCGCTG GAGACTCTCT CGGTCTGGCT CTGCCCCCAC CACCGGCCTC CCCAGGATCT
    CGGACCAGCC CTCAGGAGCT GTCAGAGGAA CTAAGCAGAA GGCTTCAGAT CACTCCAGAC
    TCCAATGGGG AACAGTTCAG CTCTTTGATT CAAAGAGAAC CCTCCTCAAG GTTGAGGTCA
    TGCAGTGTCA CCGACGCAGT TGCAGAACAG GGCCATCTAC CACCGCCCAG TGCCCCAGCT
    GGGAGAGCGC GTTCATCAAC TGTCACGGGT GGTGAGGAAC CAACGCCATC AGTGGCCTAT
    GTACATACCA CGCCGGGTCT GCCTTCAGGC TGGGAAGAAA GAAAAGATGC TAAGGGGCGC
    ACATACTATG TCAATCATAA CAATCGAACC ACAACTTGGA CTCGACCTAT CATGCAGCTT
    GCAGAAGATG GTGCGTCCGG ATCAGCCACA AACAGTAACA ACCATCTAAT CGAGCCTCAG
    ATCCGCCGGC CTCGTAGCCT CAGCTCGCCA ACAGTAACTT TATCTGCCCC GCTGGAGGGT
    GCCAAGGACT CACCCGTACG TCGGGCTGTG AAAGACACCC TTTCCAACCC ACAGTCCCCA
    CAGCCATCAC CTTACAACTC CCCCAAACCA CAACACAAAG TCACACAGAG CTTCTTGCCA
    CCCGGCTGGG AAATGAGGAT AGCGCCAAAC GGCCGGCCCT TCTTCATTGA TCATAACACA
    AAGACTACAA CCTGGGAAGA TCCACGTTTG AAATTTCCAG TACATATGCG GTCAAAGACA
    TCTTTAAACC CCAATGACCT TGGCCCCCTT CCTCCTGGCT GGGAAGAAAG AATTCACTTG
    GATGGCCGAA CGTTTTATAT TGATCATAGC AGCCAGGTGT TATGTGGAGA AAATGATACC
    AGAGAATCAG TGCCTTCATA CGATAGCAAA ATTACTCAGT GGGAAGACCC AAGACTGCAG
    AACCCAGCTA TTACTGGTCC GGCTGTCCCT TACTCCAGAG AATTTAAGCA GAAATATGAC
    TACTTCAGGA AGAAATTAAA GAAACCTGCT GATATCCCCA ATAGGTTTGA AATGAAACTT
    CACAGAAATA ACATATTTGA AGAGTCCTAT CGGAGAATTA TGTCCGTGAA AAGACCAGAT
    GTCCTAAAAG CTAGACTGTG GATTGAGTTT GAATCAGAGA AAGGTCTTGA CTATGGGGGT
    GTGGCCAGAG AATGGTTCTT CTTACTGTCC AAAGAGATGT TCAACCCCTA CTACGGCCTC
    TTTGAGTACT CTGCCACGGA CAACTACACC CTTCAGATCA ACCCTAATTC AGGCCTCTGT
    AATGAGGATC ATTTGTCCTA CTTCACTTTT ATTGGAAGAG TTGCTGGTCT GGCCGTATTT
    CATGGGAAGC TCTTAGATGG TTTCTTCATT AGACCATTTT ACAAGATGAT GTTGGGAAAG
    CAGATAACCC TGAATGACAT GGAATCTGTG GATAGTGAAT ATTACAACTC TTTGAAATGG
    ATCCTGGAGA ATGACCCTAC TGAGCTGGAC CTCATGTTCT GCATAGACGA AGAAAACTTT
    GGACAGACAT ATCAAGTGGA TTTGAAGCCC AATGGGTCAG AAATAATGGT CACAAATGAA
    AACAAAAGGG AATATATCGA CTTAGTCATC CAGTGGAGAT TTGTGAACAG GGTCCAGAAG
    CAGATGAACG CCTTCTTGGA GGGATTCACA GAACTACTTC CTATTGATTT GATTAAAATT
    TTTGATGAAA ATGAGCTGGA GTTGCTCATG TGCGGCCTCG GTGATGTGGA TGTGAATGAC
    TGGAGACAGC ATTCTATTTA CAAGAACGGC TACTGCCCAA ACCACCCCGT CATTCAGTGG
    TTCTGGAAGG CTGTGCTACT CATGGACGCC GAAAAGCGTA TCCGGTTACT GCAGTTTGTC
    ACAGGGACAT CGCGAGTACC TATGAATGGA TTTGCCGAAC TTTATGGTTC CAATGGTCCT
    CAGCTGTTTA CAATAGAGCA ATGGGGCAGT CCTGAGAAAC TGCCCAGAGC TCACACATGC
    TTTAATCGCC TTGACTTACC TCCATATGAA ACCTTTGAAG ATTTACGAGA GAAACTTCTC
    ATGGCCGTGG AAAATGCTCA AGGATTTGAA GGGGTGGATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006722491.1
    CDS428..3409
    Translation

    Target ORF information:

    RefSeq Version XM_006722428.4
    Organism Homo sapiens(human)
    Definition Homo sapiens NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006722428.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCGACCG GGCTCGGGGA GCCGGTCTAT GGACTTTCCG AAGACGAGGG AGAGTCCCGT 
    ATTCTCAGAG TAAAAGTTGT TTCTGGAATT GATCTCGCCA AAAAGGACAT CTTTGGAGCC
    AGTGATCCGT ATGTGAAACT TTCATTGTAC GTAGCGGATG AGAATAGAGA ACTTGCTTTG
    GTCCAGACAA AAACAATTAA AAAGACACTG AACCCAAAAT GGAATGAAGA ATTTTATTTC
    AGGGTAAACC CATCTAATCA CAGACTCCTA TTTGAAGTAT TTGACGAAAA TAGACTGACA
    CGAGACGACT TCCTGGGCCA GGTGGACGTG CCCCTTAGTC ACCTTCCGAC AGAAGATCCA
    ACCATGGAGC GACCCTATAC ATTTAAGGAC TTTCTCCTCA GACCAAGAAG TCATAAGTCT
    CGAGTTAAGG GATTTTTGCG ATTGAAAATG GCCTATATGC CAAAAAATGG AGGTCAAGAT
    GAAGAAAACA GTGACCAGAG GGATGACATG GAGCATGGAT GGGAAGTTGT TGACTCAAAT
    GACTCGGCTT CTCAGCACCA AGAGGAACTT CCTCCTCCTC CTCTGCCTCC CGGGTGGGAA
    GAAAAAGTGG ACAATTTAGG CCGAACTTAC TATGTCAACC ACAACAACCG GACCACTCAG
    TGGCACAGAC CAAGCCTGAT GGACGTGTCC TCGGAGTCGG ACAATAACAT CAGACAGATC
    AACCAGGAGG CAGCACACCG GCGCTTCCGC TCCCGCAGGC ACATCAGCGA AGACTTGGAG
    CCCGAGCCCT CGGAGGGCGG GGATGTCCCC GAGCCTTGGG AGACCATTTC AGAGGAAGTG
    AATATCGCTG GAGACTCTCT CGGTCTGGCT CTGCCCCCAC CACCGGCCTC CCCAGGATCT
    CGGACCAGCC CTCAGGAGCT GTCAGAGGAA CTAAGCAGAA GGCTTCAGAT CACTCCAGAC
    TCCAATGGGG AACAGTTCAG CTCTTTGATT CAAAGAGAAC CCTCCTCAAG GTTGAGGTCA
    TGCAGTGTCA CCGACGCAGT TGCAGAACAG GGCCATCTAC CACCGCCCAG TGCCCCAGCT
    GGGAGAGCGC GTTCATCAAC TGTCACGGGT GGTGAGGAAC CAACGCCATC AGTGGCCTAT
    GTACATACCA CGCCGGGTCT GCCTTCAGGC TGGGAAGAAA GAAAAGATGC TAAGGGGCGC
    ACATACTATG TCAATCATAA CAATCGAACC ACAACTTGGA CTCGACCTAT CATGCAGCTT
    GCAGAAGATG GTGCGTCCGG ATCAGCCACA AACAGTAACA ACCATCTAAT CGAGCCTCAG
    ATCCGCCGGC CTCGTAGCCT CAGCTCGCCA ACAGTAACTT TATCTGCCCC GCTGGAGGGT
    GCCAAGGACT CACCCGTACG TCGGGCTGTG AAAGACACCC TTTCCAACCC ACAGTCCCCA
    CAGCCATCAC CTTACAACTC CCCCAAACCA CAACACAAAG TCACACAGAG CTTCTTGCCA
    CCCGGCTGGG AAATGAGGAT AGCGCCAAAC GGCCGGCCCT TCTTCATTGA TCATAACACA
    AAGACTACAA CCTGGGAAGA TCCACGTTTG AAATTTCCAG TACATATGCG GTCAAAGACA
    TCTTTAAACC CCAATGACCT TGGCCCCCTT CCTCCTGGCT GGGAAGAAAG AATTCACTTG
    GATGGCCGAA CGTTTTATAT TGATCATAGC AGCCAGGTGT TATGTGGAGA AAATGATACC
    AGAGAATCAG TGCCTTCATA CGATAGCAAA ATTACTCAGT GGGAAGACCC AAGACTGCAG
    AACCCAGCTA TTACTGGTCC GGCTGTCCCT TACTCCAGAG AATTTAAGCA GAAATATGAC
    TACTTCAGGA AGAAATTAAA GAAACCTGCT GATATCCCCA ATAGGTTTGA AATGAAACTT
    CACAGAAATA ACATATTTGA AGAGTCCTAT CGGAGAATTA TGTCCGTGAA AAGACCAGAT
    GTCCTAAAAG CTAGACTGTG GATTGAGTTT GAATCAGAGA AAGGTCTTGA CTATGGGGGT
    GTGGCCAGAG AATGGTTCTT CTTACTGTCC AAAGAGATGT TCAACCCCTA CTACGGCCTC
    TTTGAGTACT CTGCCACGGA CAACTACACC CTTCAGATCA ACCCTAATTC AGGCCTCTGT
    AATGAGGATC ATTTGTCCTA CTTCACTTTT ATTGGAAGAG TTGCTGGTCT GGCCGTATTT
    CATGGGAAGC TCTTAGATGG TTTCTTCATT AGACCATTTT ACAAGATGAT GTTGGGAAAG
    CAGATAACCC TGAATGACAT GGAATCTGTG GATAGTGAAT ATTACAACTC TTTGAAATGG
    ATCCTGGAGA ATGACCCTAC TGAGCTGGAC CTCATGTTCT GCATAGACGA AGAAAACTTT
    GGACAGACAT ATCAAGTGGA TTTGAAGCCC AATGGGTCAG AAATAATGGT CACAAATGAA
    AACAAAAGGG AATATATCGA CTTAGTCATC CAGTGGAGAT TTGTGAACAG GGTCCAGAAG
    CAGATGAACG CCTTCTTGGA GGGATTCACA GAACTACTTC CTATTGATTT GATTAAAATT
    TTTGATGAAA ATGAGCTGGA GTTGCTCATG TGCGGCCTCG GTGATGTGGA TGTGAATGAC
    TGGAGACAGC ATTCTATTTA CAAGAACGGC TACTGCCCAA ACCACCCCGT CATTCAGTGG
    TTCTGGAAGG CTGTGCTACT CATGGACGCC GAAAAGCGTA TCCGGTTACT GCAGTTTGTC
    ACAGGGACAT CGCGAGTACC TATGAATGGA TTTGCCGAAC TTTATGGTTC CAATGGTCCT
    CAGCTGTTTA CAATAGAGCA ATGGGGCAGT CCTGAGAAAC TGCCCAGAGC TCACACATGC
    TTTAATCGCC TTGACTTACC TCCATATGAA ACCTTTGAAG ATTTACGAGA GAAACTTCTC
    ATGGCCGTGG AAAATGCTCA AGGATTTGAA GGGGTGGATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu51148
    Clone ID Related Accession (Same CDS sequence) XM_024451133.1 , XM_024451134.1 , XM_006722430.3 , XM_006722430.4
    Accession Version XM_024451133.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2619bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGCGAC CCTATACATT TAAGGACTTT CTCCTCAGAC CAAGAAGTCA TAAGTCTCGA 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCAA AAAATGGAGG TCAAGATGAA
    GAAAACAGTG ACCAGAGGGA TGACATGGAG CATGGATGGG AAGTTGTTGA CTCAAATGAC
    TCGGCTTCTC AGCACCAAGA GGAACTTCCT CCTCCTCCTC TGCCTCCCGG GTGGGAAGAA
    AAAGTGGACA ATTTAGGCCG AACTTACTAT GTCAACCACA ACAACCGGAC CACTCAGTGG
    CACAGACCAA GCCTGATGGA CGTGTCCTCG GAGTCGGACA ATAACATCAG ACAGATCAAC
    CAGGAGGCAG CACACCGGCG CTTCCGCTCC CGCAGGCACA TCAGCGAAGA CTTGGAGCCC
    GAGCCCTCGG AGGGCGGGGA TGTCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAT
    ATCGCTGGAG ACTCTCTCGG TCTGGCTCTG CCCCCACCAC CGGCCTCCCC AGGATCTCGG
    ACCAGCCCTC AGGAGCTGTC AGAGGAACTA AGCAGAAGGC TTCAGATCAC TCCAGACTCC
    AATGGGGAAC AGTTCAGCTC TTTGATTCAA AGAGAACCCT CCTCAAGGTT GAGGTCATGC
    AGTGTCACCG ACGCAGTTGC AGAACAGGGC CATCTACCAC CGCCCAGTGC CCCAGCTGGG
    AGAGCGCGTT CATCAACTGT CACGGGTGGT GAGGAACCAA CGCCATCAGT GGCCTATGTA
    CATACCACGC CGGGTCTGCC TTCAGGCTGG GAAGAAAGAA AAGATGCTAA GGGGCGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GACCTATCAT GCAGCTTGCA
    GAAGATGGTG CGTCCGGATC AGCCACAAAC AGTAACAACC ATCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CTGCCCCGCT GGAGGGTGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GACACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CTTGCCACCC
    GGCTGGGAAA TGAGGATAGC GCCAAACGGC CGGCCCTTCT TCATTGATCA TAACACAAAG
    ACTACAACCT GGGAAGATCC ACGTTTGAAA TTTCCAGTAC ATATGCGGTC AAAGACATCT
    TTAAACCCCA ATGACCTTGG CCCCCTTCCT CCTGGCTGGG AAGAAAGAAT TCACTTGGAT
    GGCCGAACGT TTTATATTGA TCATAGCAGC CAGGTGTTAT GTGGAGAAAA TGATACCAGA
    GAATCAGTGC CTTCATACGA TAGCAAAATT ACTCAGTGGG AAGACCCAAG ACTGCAGAAC
    CCAGCTATTA CTGGTCCGGC TGTCCCTTAC TCCAGAGAAT TTAAGCAGAA ATATGACTAC
    TTCAGGAAGA AATTAAAGAA ACCTGCTGAT ATCCCCAATA GGTTTGAAAT GAAACTTCAC
    AGAAATAACA TATTTGAAGA GTCCTATCGG AGAATTATGT CCGTGAAAAG ACCAGATGTC
    CTAAAAGCTA GACTGTGGAT TGAGTTTGAA TCAGAGAAAG GTCTTGACTA TGGGGGTGTG
    GCCAGAGAAT GGTTCTTCTT ACTGTCCAAA GAGATGTTCA ACCCCTACTA CGGCCTCTTT
    GAGTACTCTG CCACGGACAA CTACACCCTT CAGATCAACC CTAATTCAGG CCTCTGTAAT
    GAGGATCATT TGTCCTACTT CACTTTTATT GGAAGAGTTG CTGGTCTGGC CGTATTTCAT
    GGGAAGCTCT TAGATGGTTT CTTCATTAGA CCATTTTACA AGATGATGTT GGGAAAGCAG
    ATAACCCTGA ATGACATGGA ATCTGTGGAT AGTGAATATT ACAACTCTTT GAAATGGATC
    CTGGAGAATG ACCCTACTGA GCTGGACCTC ATGTTCTGCA TAGACGAAGA AAACTTTGGA
    CAGACATATC AAGTGGATTT GAAGCCCAAT GGGTCAGAAA TAATGGTCAC AAATGAAAAC
    AAAAGGGAAT ATATCGACTT AGTCATCCAG TGGAGATTTG TGAACAGGGT CCAGAAGCAG
    ATGAACGCCT TCTTGGAGGG ATTCACAGAA CTACTTCCTA TTGATTTGAT TAAAATTTTT
    GATGAAAATG AGCTGGAGTT GCTCATGTGC GGCCTCGGTG ATGTGGATGT GAATGACTGG
    AGACAGCATT CTATTTACAA GAACGGCTAC TGCCCAAACC ACCCCGTCAT TCAGTGGTTC
    TGGAAGGCTG TGCTACTCAT GGACGCCGAA AAGCGTATCC GGTTACTGCA GTTTGTCACA
    GGGACATCGC GAGTACCTAT GAATGGATTT GCCGAACTTT ATGGTTCCAA TGGTCCTCAG
    CTGTTTACAA TAGAGCAATG GGGCAGTCCT GAGAAACTGC CCAGAGCTCA CACATGCTTT
    AATCGCCTTG ACTTACCTCC ATATGAAACC TTTGAAGATT TACGAGAGAA ACTTCTCATG
    GCCGTGGAAA ATGCTCAAGG ATTTGAAGGG GTGGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024306901.1
    CDS846..3464
    Translation

    Target ORF information:

    RefSeq Version XM_024451133.1
    Organism Homo sapiens(human)
    Definition Homo sapiens NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024451133.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGCGAC CCTATACATT TAAGGACTTT CTCCTCAGAC CAAGAAGTCA TAAGTCTCGA 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCAA AAAATGGAGG TCAAGATGAA
    GAAAACAGTG ACCAGAGGGA TGACATGGAG CATGGATGGG AAGTTGTTGA CTCAAATGAC
    TCGGCTTCTC AGCACCAAGA GGAACTTCCT CCTCCTCCTC TGCCTCCCGG GTGGGAAGAA
    AAAGTGGACA ATTTAGGCCG AACTTACTAT GTCAACCACA ACAACCGGAC CACTCAGTGG
    CACAGACCAA GCCTGATGGA CGTGTCCTCG GAGTCGGACA ATAACATCAG ACAGATCAAC
    CAGGAGGCAG CACACCGGCG CTTCCGCTCC CGCAGGCACA TCAGCGAAGA CTTGGAGCCC
    GAGCCCTCGG AGGGCGGGGA TGTCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAT
    ATCGCTGGAG ACTCTCTCGG TCTGGCTCTG CCCCCACCAC CGGCCTCCCC AGGATCTCGG
    ACCAGCCCTC AGGAGCTGTC AGAGGAACTA AGCAGAAGGC TTCAGATCAC TCCAGACTCC
    AATGGGGAAC AGTTCAGCTC TTTGATTCAA AGAGAACCCT CCTCAAGGTT GAGGTCATGC
    AGTGTCACCG ACGCAGTTGC AGAACAGGGC CATCTACCAC CGCCCAGTGC CCCAGCTGGG
    AGAGCGCGTT CATCAACTGT CACGGGTGGT GAGGAACCAA CGCCATCAGT GGCCTATGTA
    CATACCACGC CGGGTCTGCC TTCAGGCTGG GAAGAAAGAA AAGATGCTAA GGGGCGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GACCTATCAT GCAGCTTGCA
    GAAGATGGTG CGTCCGGATC AGCCACAAAC AGTAACAACC ATCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CTGCCCCGCT GGAGGGTGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GACACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CTTGCCACCC
    GGCTGGGAAA TGAGGATAGC GCCAAACGGC CGGCCCTTCT TCATTGATCA TAACACAAAG
    ACTACAACCT GGGAAGATCC ACGTTTGAAA TTTCCAGTAC ATATGCGGTC AAAGACATCT
    TTAAACCCCA ATGACCTTGG CCCCCTTCCT CCTGGCTGGG AAGAAAGAAT TCACTTGGAT
    GGCCGAACGT TTTATATTGA TCATAGCAGC CAGGTGTTAT GTGGAGAAAA TGATACCAGA
    GAATCAGTGC CTTCATACGA TAGCAAAATT ACTCAGTGGG AAGACCCAAG ACTGCAGAAC
    CCAGCTATTA CTGGTCCGGC TGTCCCTTAC TCCAGAGAAT TTAAGCAGAA ATATGACTAC
    TTCAGGAAGA AATTAAAGAA ACCTGCTGAT ATCCCCAATA GGTTTGAAAT GAAACTTCAC
    AGAAATAACA TATTTGAAGA GTCCTATCGG AGAATTATGT CCGTGAAAAG ACCAGATGTC
    CTAAAAGCTA GACTGTGGAT TGAGTTTGAA TCAGAGAAAG GTCTTGACTA TGGGGGTGTG
    GCCAGAGAAT GGTTCTTCTT ACTGTCCAAA GAGATGTTCA ACCCCTACTA CGGCCTCTTT
    GAGTACTCTG CCACGGACAA CTACACCCTT CAGATCAACC CTAATTCAGG CCTCTGTAAT
    GAGGATCATT TGTCCTACTT CACTTTTATT GGAAGAGTTG CTGGTCTGGC CGTATTTCAT
    GGGAAGCTCT TAGATGGTTT CTTCATTAGA CCATTTTACA AGATGATGTT GGGAAAGCAG
    ATAACCCTGA ATGACATGGA ATCTGTGGAT AGTGAATATT ACAACTCTTT GAAATGGATC
    CTGGAGAATG ACCCTACTGA GCTGGACCTC ATGTTCTGCA TAGACGAAGA AAACTTTGGA
    CAGACATATC AAGTGGATTT GAAGCCCAAT GGGTCAGAAA TAATGGTCAC AAATGAAAAC
    AAAAGGGAAT ATATCGACTT AGTCATCCAG TGGAGATTTG TGAACAGGGT CCAGAAGCAG
    ATGAACGCCT TCTTGGAGGG ATTCACAGAA CTACTTCCTA TTGATTTGAT TAAAATTTTT
    GATGAAAATG AGCTGGAGTT GCTCATGTGC GGCCTCGGTG ATGTGGATGT GAATGACTGG
    AGACAGCATT CTATTTACAA GAACGGCTAC TGCCCAAACC ACCCCGTCAT TCAGTGGTTC
    TGGAAGGCTG TGCTACTCAT GGACGCCGAA AAGCGTATCC GGTTACTGCA GTTTGTCACA
    GGGACATCGC GAGTACCTAT GAATGGATTT GCCGAACTTT ATGGTTCCAA TGGTCCTCAG
    CTGTTTACAA TAGAGCAATG GGGCAGTCCT GAGAAACTGC CCAGAGCTCA CACATGCTTT
    AATCGCCTTG ACTTACCTCC ATATGAAACC TTTGAAGATT TACGAGAGAA ACTTCTCATG
    GCCGTGGAAA ATGCTCAAGG ATTTGAAGGG GTGGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu51148
    Clone ID Related Accession (Same CDS sequence) XM_024451133.1 , XM_024451134.1 , XM_006722430.3 , XM_006722430.4
    Accession Version XM_024451134.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2619bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGCGAC CCTATACATT TAAGGACTTT CTCCTCAGAC CAAGAAGTCA TAAGTCTCGA 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCAA AAAATGGAGG TCAAGATGAA
    GAAAACAGTG ACCAGAGGGA TGACATGGAG CATGGATGGG AAGTTGTTGA CTCAAATGAC
    TCGGCTTCTC AGCACCAAGA GGAACTTCCT CCTCCTCCTC TGCCTCCCGG GTGGGAAGAA
    AAAGTGGACA ATTTAGGCCG AACTTACTAT GTCAACCACA ACAACCGGAC CACTCAGTGG
    CACAGACCAA GCCTGATGGA CGTGTCCTCG GAGTCGGACA ATAACATCAG ACAGATCAAC
    CAGGAGGCAG CACACCGGCG CTTCCGCTCC CGCAGGCACA TCAGCGAAGA CTTGGAGCCC
    GAGCCCTCGG AGGGCGGGGA TGTCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAT
    ATCGCTGGAG ACTCTCTCGG TCTGGCTCTG CCCCCACCAC CGGCCTCCCC AGGATCTCGG
    ACCAGCCCTC AGGAGCTGTC AGAGGAACTA AGCAGAAGGC TTCAGATCAC TCCAGACTCC
    AATGGGGAAC AGTTCAGCTC TTTGATTCAA AGAGAACCCT CCTCAAGGTT GAGGTCATGC
    AGTGTCACCG ACGCAGTTGC AGAACAGGGC CATCTACCAC CGCCCAGTGC CCCAGCTGGG
    AGAGCGCGTT CATCAACTGT CACGGGTGGT GAGGAACCAA CGCCATCAGT GGCCTATGTA
    CATACCACGC CGGGTCTGCC TTCAGGCTGG GAAGAAAGAA AAGATGCTAA GGGGCGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GACCTATCAT GCAGCTTGCA
    GAAGATGGTG CGTCCGGATC AGCCACAAAC AGTAACAACC ATCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CTGCCCCGCT GGAGGGTGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GACACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CTTGCCACCC
    GGCTGGGAAA TGAGGATAGC GCCAAACGGC CGGCCCTTCT TCATTGATCA TAACACAAAG
    ACTACAACCT GGGAAGATCC ACGTTTGAAA TTTCCAGTAC ATATGCGGTC AAAGACATCT
    TTAAACCCCA ATGACCTTGG CCCCCTTCCT CCTGGCTGGG AAGAAAGAAT TCACTTGGAT
    GGCCGAACGT TTTATATTGA TCATAGCAGC CAGGTGTTAT GTGGAGAAAA TGATACCAGA
    GAATCAGTGC CTTCATACGA TAGCAAAATT ACTCAGTGGG AAGACCCAAG ACTGCAGAAC
    CCAGCTATTA CTGGTCCGGC TGTCCCTTAC TCCAGAGAAT TTAAGCAGAA ATATGACTAC
    TTCAGGAAGA AATTAAAGAA ACCTGCTGAT ATCCCCAATA GGTTTGAAAT GAAACTTCAC
    AGAAATAACA TATTTGAAGA GTCCTATCGG AGAATTATGT CCGTGAAAAG ACCAGATGTC
    CTAAAAGCTA GACTGTGGAT TGAGTTTGAA TCAGAGAAAG GTCTTGACTA TGGGGGTGTG
    GCCAGAGAAT GGTTCTTCTT ACTGTCCAAA GAGATGTTCA ACCCCTACTA CGGCCTCTTT
    GAGTACTCTG CCACGGACAA CTACACCCTT CAGATCAACC CTAATTCAGG CCTCTGTAAT
    GAGGATCATT TGTCCTACTT CACTTTTATT GGAAGAGTTG CTGGTCTGGC CGTATTTCAT
    GGGAAGCTCT TAGATGGTTT CTTCATTAGA CCATTTTACA AGATGATGTT GGGAAAGCAG
    ATAACCCTGA ATGACATGGA ATCTGTGGAT AGTGAATATT ACAACTCTTT GAAATGGATC
    CTGGAGAATG ACCCTACTGA GCTGGACCTC ATGTTCTGCA TAGACGAAGA AAACTTTGGA
    CAGACATATC AAGTGGATTT GAAGCCCAAT GGGTCAGAAA TAATGGTCAC AAATGAAAAC
    AAAAGGGAAT ATATCGACTT AGTCATCCAG TGGAGATTTG TGAACAGGGT CCAGAAGCAG
    ATGAACGCCT TCTTGGAGGG ATTCACAGAA CTACTTCCTA TTGATTTGAT TAAAATTTTT
    GATGAAAATG AGCTGGAGTT GCTCATGTGC GGCCTCGGTG ATGTGGATGT GAATGACTGG
    AGACAGCATT CTATTTACAA GAACGGCTAC TGCCCAAACC ACCCCGTCAT TCAGTGGTTC
    TGGAAGGCTG TGCTACTCAT GGACGCCGAA AAGCGTATCC GGTTACTGCA GTTTGTCACA
    GGGACATCGC GAGTACCTAT GAATGGATTT GCCGAACTTT ATGGTTCCAA TGGTCCTCAG
    CTGTTTACAA TAGAGCAATG GGGCAGTCCT GAGAAACTGC CCAGAGCTCA CACATGCTTT
    AATCGCCTTG ACTTACCTCC ATATGAAACC TTTGAAGATT TACGAGAGAA ACTTCTCATG
    GCCGTGGAAA ATGCTCAAGG ATTTGAAGGG GTGGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024306902.1
    CDS705..3323
    Translation

    Target ORF information:

    RefSeq Version XM_024451134.1
    Organism Homo sapiens(human)
    Definition Homo sapiens NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024451134.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGCGAC CCTATACATT TAAGGACTTT CTCCTCAGAC CAAGAAGTCA TAAGTCTCGA 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCAA AAAATGGAGG TCAAGATGAA
    GAAAACAGTG ACCAGAGGGA TGACATGGAG CATGGATGGG AAGTTGTTGA CTCAAATGAC
    TCGGCTTCTC AGCACCAAGA GGAACTTCCT CCTCCTCCTC TGCCTCCCGG GTGGGAAGAA
    AAAGTGGACA ATTTAGGCCG AACTTACTAT GTCAACCACA ACAACCGGAC CACTCAGTGG
    CACAGACCAA GCCTGATGGA CGTGTCCTCG GAGTCGGACA ATAACATCAG ACAGATCAAC
    CAGGAGGCAG CACACCGGCG CTTCCGCTCC CGCAGGCACA TCAGCGAAGA CTTGGAGCCC
    GAGCCCTCGG AGGGCGGGGA TGTCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAT
    ATCGCTGGAG ACTCTCTCGG TCTGGCTCTG CCCCCACCAC CGGCCTCCCC AGGATCTCGG
    ACCAGCCCTC AGGAGCTGTC AGAGGAACTA AGCAGAAGGC TTCAGATCAC TCCAGACTCC
    AATGGGGAAC AGTTCAGCTC TTTGATTCAA AGAGAACCCT CCTCAAGGTT GAGGTCATGC
    AGTGTCACCG ACGCAGTTGC AGAACAGGGC CATCTACCAC CGCCCAGTGC CCCAGCTGGG
    AGAGCGCGTT CATCAACTGT CACGGGTGGT GAGGAACCAA CGCCATCAGT GGCCTATGTA
    CATACCACGC CGGGTCTGCC TTCAGGCTGG GAAGAAAGAA AAGATGCTAA GGGGCGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GACCTATCAT GCAGCTTGCA
    GAAGATGGTG CGTCCGGATC AGCCACAAAC AGTAACAACC ATCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CTGCCCCGCT GGAGGGTGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GACACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CTTGCCACCC
    GGCTGGGAAA TGAGGATAGC GCCAAACGGC CGGCCCTTCT TCATTGATCA TAACACAAAG
    ACTACAACCT GGGAAGATCC ACGTTTGAAA TTTCCAGTAC ATATGCGGTC AAAGACATCT
    TTAAACCCCA ATGACCTTGG CCCCCTTCCT CCTGGCTGGG AAGAAAGAAT TCACTTGGAT
    GGCCGAACGT TTTATATTGA TCATAGCAGC CAGGTGTTAT GTGGAGAAAA TGATACCAGA
    GAATCAGTGC CTTCATACGA TAGCAAAATT ACTCAGTGGG AAGACCCAAG ACTGCAGAAC
    CCAGCTATTA CTGGTCCGGC TGTCCCTTAC TCCAGAGAAT TTAAGCAGAA ATATGACTAC
    TTCAGGAAGA AATTAAAGAA ACCTGCTGAT ATCCCCAATA GGTTTGAAAT GAAACTTCAC
    AGAAATAACA TATTTGAAGA GTCCTATCGG AGAATTATGT CCGTGAAAAG ACCAGATGTC
    CTAAAAGCTA GACTGTGGAT TGAGTTTGAA TCAGAGAAAG GTCTTGACTA TGGGGGTGTG
    GCCAGAGAAT GGTTCTTCTT ACTGTCCAAA GAGATGTTCA ACCCCTACTA CGGCCTCTTT
    GAGTACTCTG CCACGGACAA CTACACCCTT CAGATCAACC CTAATTCAGG CCTCTGTAAT
    GAGGATCATT TGTCCTACTT CACTTTTATT GGAAGAGTTG CTGGTCTGGC CGTATTTCAT
    GGGAAGCTCT TAGATGGTTT CTTCATTAGA CCATTTTACA AGATGATGTT GGGAAAGCAG
    ATAACCCTGA ATGACATGGA ATCTGTGGAT AGTGAATATT ACAACTCTTT GAAATGGATC
    CTGGAGAATG ACCCTACTGA GCTGGACCTC ATGTTCTGCA TAGACGAAGA AAACTTTGGA
    CAGACATATC AAGTGGATTT GAAGCCCAAT GGGTCAGAAA TAATGGTCAC AAATGAAAAC
    AAAAGGGAAT ATATCGACTT AGTCATCCAG TGGAGATTTG TGAACAGGGT CCAGAAGCAG
    ATGAACGCCT TCTTGGAGGG ATTCACAGAA CTACTTCCTA TTGATTTGAT TAAAATTTTT
    GATGAAAATG AGCTGGAGTT GCTCATGTGC GGCCTCGGTG ATGTGGATGT GAATGACTGG
    AGACAGCATT CTATTTACAA GAACGGCTAC TGCCCAAACC ACCCCGTCAT TCAGTGGTTC
    TGGAAGGCTG TGCTACTCAT GGACGCCGAA AAGCGTATCC GGTTACTGCA GTTTGTCACA
    GGGACATCGC GAGTACCTAT GAATGGATTT GCCGAACTTT ATGGTTCCAA TGGTCCTCAG
    CTGTTTACAA TAGAGCAATG GGGCAGTCCT GAGAAACTGC CCAGAGCTCA CACATGCTTT
    AATCGCCTTG ACTTACCTCC ATATGAAACC TTTGAAGATT TACGAGAGAA ACTTCTCATG
    GCCGTGGAAA ATGCTCAAGG ATTTGAAGGG GTGGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu51148
    Clone ID Related Accession (Same CDS sequence) XM_024451133.1 , XM_024451134.1 , XM_006722430.3 , XM_006722430.4
    Accession Version XM_006722430.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2619bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006722430.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGCGAC CCTATACATT TAAGGACTTT CTCCTCAGAC CAAGAAGTCA TAAGTCTCGA 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCAA AAAATGGAGG TCAAGATGAA
    GAAAACAGTG ACCAGAGGGA TGACATGGAG CATGGATGGG AAGTTGTTGA CTCAAATGAC
    TCGGCTTCTC AGCACCAAGA GGAACTTCCT CCTCCTCCTC TGCCTCCCGG GTGGGAAGAA
    AAAGTGGACA ATTTAGGCCG AACTTACTAT GTCAACCACA ACAACCGGAC CACTCAGTGG
    CACAGACCAA GCCTGATGGA CGTGTCCTCG GAGTCGGACA ATAACATCAG ACAGATCAAC
    CAGGAGGCAG CACACCGGCG CTTCCGCTCC CGCAGGCACA TCAGCGAAGA CTTGGAGCCC
    GAGCCCTCGG AGGGCGGGGA TGTCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAT
    ATCGCTGGAG ACTCTCTCGG TCTGGCTCTG CCCCCACCAC CGGCCTCCCC AGGATCTCGG
    ACCAGCCCTC AGGAGCTGTC AGAGGAACTA AGCAGAAGGC TTCAGATCAC TCCAGACTCC
    AATGGGGAAC AGTTCAGCTC TTTGATTCAA AGAGAACCCT CCTCAAGGTT GAGGTCATGC
    AGTGTCACCG ACGCAGTTGC AGAACAGGGC CATCTACCAC CGCCCAGTGC CCCAGCTGGG
    AGAGCGCGTT CATCAACTGT CACGGGTGGT GAGGAACCAA CGCCATCAGT GGCCTATGTA
    CATACCACGC CGGGTCTGCC TTCAGGCTGG GAAGAAAGAA AAGATGCTAA GGGGCGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GACCTATCAT GCAGCTTGCA
    GAAGATGGTG CGTCCGGATC AGCCACAAAC AGTAACAACC ATCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CTGCCCCGCT GGAGGGTGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GACACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CTTGCCACCC
    GGCTGGGAAA TGAGGATAGC GCCAAACGGC CGGCCCTTCT TCATTGATCA TAACACAAAG
    ACTACAACCT GGGAAGATCC ACGTTTGAAA TTTCCAGTAC ATATGCGGTC AAAGACATCT
    TTAAACCCCA ATGACCTTGG CCCCCTTCCT CCTGGCTGGG AAGAAAGAAT TCACTTGGAT
    GGCCGAACGT TTTATATTGA TCATAGCAGC CAGGTGTTAT GTGGAGAAAA TGATACCAGA
    GAATCAGTGC CTTCATACGA TAGCAAAATT ACTCAGTGGG AAGACCCAAG ACTGCAGAAC
    CCAGCTATTA CTGGTCCGGC TGTCCCTTAC TCCAGAGAAT TTAAGCAGAA ATATGACTAC
    TTCAGGAAGA AATTAAAGAA ACCTGCTGAT ATCCCCAATA GGTTTGAAAT GAAACTTCAC
    AGAAATAACA TATTTGAAGA GTCCTATCGG AGAATTATGT CCGTGAAAAG ACCAGATGTC
    CTAAAAGCTA GACTGTGGAT TGAGTTTGAA TCAGAGAAAG GTCTTGACTA TGGGGGTGTG
    GCCAGAGAAT GGTTCTTCTT ACTGTCCAAA GAGATGTTCA ACCCCTACTA CGGCCTCTTT
    GAGTACTCTG CCACGGACAA CTACACCCTT CAGATCAACC CTAATTCAGG CCTCTGTAAT
    GAGGATCATT TGTCCTACTT CACTTTTATT GGAAGAGTTG CTGGTCTGGC CGTATTTCAT
    GGGAAGCTCT TAGATGGTTT CTTCATTAGA CCATTTTACA AGATGATGTT GGGAAAGCAG
    ATAACCCTGA ATGACATGGA ATCTGTGGAT AGTGAATATT ACAACTCTTT GAAATGGATC
    CTGGAGAATG ACCCTACTGA GCTGGACCTC ATGTTCTGCA TAGACGAAGA AAACTTTGGA
    CAGACATATC AAGTGGATTT GAAGCCCAAT GGGTCAGAAA TAATGGTCAC AAATGAAAAC
    AAAAGGGAAT ATATCGACTT AGTCATCCAG TGGAGATTTG TGAACAGGGT CCAGAAGCAG
    ATGAACGCCT TCTTGGAGGG ATTCACAGAA CTACTTCCTA TTGATTTGAT TAAAATTTTT
    GATGAAAATG AGCTGGAGTT GCTCATGTGC GGCCTCGGTG ATGTGGATGT GAATGACTGG
    AGACAGCATT CTATTTACAA GAACGGCTAC TGCCCAAACC ACCCCGTCAT TCAGTGGTTC
    TGGAAGGCTG TGCTACTCAT GGACGCCGAA AAGCGTATCC GGTTACTGCA GTTTGTCACA
    GGGACATCGC GAGTACCTAT GAATGGATTT GCCGAACTTT ATGGTTCCAA TGGTCCTCAG
    CTGTTTACAA TAGAGCAATG GGGCAGTCCT GAGAAACTGC CCAGAGCTCA CACATGCTTT
    AATCGCCTTG ACTTACCTCC ATATGAAACC TTTGAAGATT TACGAGAGAA ACTTCTCATG
    GCCGTGGAAA ATGCTCAAGG ATTTGAAGGG GTGGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006722493.1
    CDS571..3189
    Translation

    Target ORF information:

    RefSeq Version XM_006722430.3
    Organism Homo sapiens(human)
    Definition Homo sapiens neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006722430.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGCGAC CCTATACATT TAAGGACTTT CTCCTCAGAC CAAGAAGTCA TAAGTCTCGA 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCAA AAAATGGAGG TCAAGATGAA
    GAAAACAGTG ACCAGAGGGA TGACATGGAG CATGGATGGG AAGTTGTTGA CTCAAATGAC
    TCGGCTTCTC AGCACCAAGA GGAACTTCCT CCTCCTCCTC TGCCTCCCGG GTGGGAAGAA
    AAAGTGGACA ATTTAGGCCG AACTTACTAT GTCAACCACA ACAACCGGAC CACTCAGTGG
    CACAGACCAA GCCTGATGGA CGTGTCCTCG GAGTCGGACA ATAACATCAG ACAGATCAAC
    CAGGAGGCAG CACACCGGCG CTTCCGCTCC CGCAGGCACA TCAGCGAAGA CTTGGAGCCC
    GAGCCCTCGG AGGGCGGGGA TGTCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAT
    ATCGCTGGAG ACTCTCTCGG TCTGGCTCTG CCCCCACCAC CGGCCTCCCC AGGATCTCGG
    ACCAGCCCTC AGGAGCTGTC AGAGGAACTA AGCAGAAGGC TTCAGATCAC TCCAGACTCC
    AATGGGGAAC AGTTCAGCTC TTTGATTCAA AGAGAACCCT CCTCAAGGTT GAGGTCATGC
    AGTGTCACCG ACGCAGTTGC AGAACAGGGC CATCTACCAC CGCCCAGTGC CCCAGCTGGG
    AGAGCGCGTT CATCAACTGT CACGGGTGGT GAGGAACCAA CGCCATCAGT GGCCTATGTA
    CATACCACGC CGGGTCTGCC TTCAGGCTGG GAAGAAAGAA AAGATGCTAA GGGGCGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GACCTATCAT GCAGCTTGCA
    GAAGATGGTG CGTCCGGATC AGCCACAAAC AGTAACAACC ATCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CTGCCCCGCT GGAGGGTGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GACACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CTTGCCACCC
    GGCTGGGAAA TGAGGATAGC GCCAAACGGC CGGCCCTTCT TCATTGATCA TAACACAAAG
    ACTACAACCT GGGAAGATCC ACGTTTGAAA TTTCCAGTAC ATATGCGGTC AAAGACATCT
    TTAAACCCCA ATGACCTTGG CCCCCTTCCT CCTGGCTGGG AAGAAAGAAT TCACTTGGAT
    GGCCGAACGT TTTATATTGA TCATAGCAGC CAGGTGTTAT GTGGAGAAAA TGATACCAGA
    GAATCAGTGC CTTCATACGA TAGCAAAATT ACTCAGTGGG AAGACCCAAG ACTGCAGAAC
    CCAGCTATTA CTGGTCCGGC TGTCCCTTAC TCCAGAGAAT TTAAGCAGAA ATATGACTAC
    TTCAGGAAGA AATTAAAGAA ACCTGCTGAT ATCCCCAATA GGTTTGAAAT GAAACTTCAC
    AGAAATAACA TATTTGAAGA GTCCTATCGG AGAATTATGT CCGTGAAAAG ACCAGATGTC
    CTAAAAGCTA GACTGTGGAT TGAGTTTGAA TCAGAGAAAG GTCTTGACTA TGGGGGTGTG
    GCCAGAGAAT GGTTCTTCTT ACTGTCCAAA GAGATGTTCA ACCCCTACTA CGGCCTCTTT
    GAGTACTCTG CCACGGACAA CTACACCCTT CAGATCAACC CTAATTCAGG CCTCTGTAAT
    GAGGATCATT TGTCCTACTT CACTTTTATT GGAAGAGTTG CTGGTCTGGC CGTATTTCAT
    GGGAAGCTCT TAGATGGTTT CTTCATTAGA CCATTTTACA AGATGATGTT GGGAAAGCAG
    ATAACCCTGA ATGACATGGA ATCTGTGGAT AGTGAATATT ACAACTCTTT GAAATGGATC
    CTGGAGAATG ACCCTACTGA GCTGGACCTC ATGTTCTGCA TAGACGAAGA AAACTTTGGA
    CAGACATATC AAGTGGATTT GAAGCCCAAT GGGTCAGAAA TAATGGTCAC AAATGAAAAC
    AAAAGGGAAT ATATCGACTT AGTCATCCAG TGGAGATTTG TGAACAGGGT CCAGAAGCAG
    ATGAACGCCT TCTTGGAGGG ATTCACAGAA CTACTTCCTA TTGATTTGAT TAAAATTTTT
    GATGAAAATG AGCTGGAGTT GCTCATGTGC GGCCTCGGTG ATGTGGATGT GAATGACTGG
    AGACAGCATT CTATTTACAA GAACGGCTAC TGCCCAAACC ACCCCGTCAT TCAGTGGTTC
    TGGAAGGCTG TGCTACTCAT GGACGCCGAA AAGCGTATCC GGTTACTGCA GTTTGTCACA
    GGGACATCGC GAGTACCTAT GAATGGATTT GCCGAACTTT ATGGTTCCAA TGGTCCTCAG
    CTGTTTACAA TAGAGCAATG GGGCAGTCCT GAGAAACTGC CCAGAGCTCA CACATGCTTT
    AATCGCCTTG ACTTACCTCC ATATGAAACC TTTGAAGATT TACGAGAGAA ACTTCTCATG
    GCCGTGGAAA ATGCTCAAGG ATTTGAAGGG GTGGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu51148
    Clone ID Related Accession (Same CDS sequence) XM_024451133.1 , XM_024451134.1 , XM_006722430.3 , XM_006722430.4
    Accession Version XM_006722430.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2619bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_006722430.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGCGAC CCTATACATT TAAGGACTTT CTCCTCAGAC CAAGAAGTCA TAAGTCTCGA 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCAA AAAATGGAGG TCAAGATGAA
    GAAAACAGTG ACCAGAGGGA TGACATGGAG CATGGATGGG AAGTTGTTGA CTCAAATGAC
    TCGGCTTCTC AGCACCAAGA GGAACTTCCT CCTCCTCCTC TGCCTCCCGG GTGGGAAGAA
    AAAGTGGACA ATTTAGGCCG AACTTACTAT GTCAACCACA ACAACCGGAC CACTCAGTGG
    CACAGACCAA GCCTGATGGA CGTGTCCTCG GAGTCGGACA ATAACATCAG ACAGATCAAC
    CAGGAGGCAG CACACCGGCG CTTCCGCTCC CGCAGGCACA TCAGCGAAGA CTTGGAGCCC
    GAGCCCTCGG AGGGCGGGGA TGTCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAT
    ATCGCTGGAG ACTCTCTCGG TCTGGCTCTG CCCCCACCAC CGGCCTCCCC AGGATCTCGG
    ACCAGCCCTC AGGAGCTGTC AGAGGAACTA AGCAGAAGGC TTCAGATCAC TCCAGACTCC
    AATGGGGAAC AGTTCAGCTC TTTGATTCAA AGAGAACCCT CCTCAAGGTT GAGGTCATGC
    AGTGTCACCG ACGCAGTTGC AGAACAGGGC CATCTACCAC CGCCCAGTGC CCCAGCTGGG
    AGAGCGCGTT CATCAACTGT CACGGGTGGT GAGGAACCAA CGCCATCAGT GGCCTATGTA
    CATACCACGC CGGGTCTGCC TTCAGGCTGG GAAGAAAGAA AAGATGCTAA GGGGCGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GACCTATCAT GCAGCTTGCA
    GAAGATGGTG CGTCCGGATC AGCCACAAAC AGTAACAACC ATCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CTGCCCCGCT GGAGGGTGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GACACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CTTGCCACCC
    GGCTGGGAAA TGAGGATAGC GCCAAACGGC CGGCCCTTCT TCATTGATCA TAACACAAAG
    ACTACAACCT GGGAAGATCC ACGTTTGAAA TTTCCAGTAC ATATGCGGTC AAAGACATCT
    TTAAACCCCA ATGACCTTGG CCCCCTTCCT CCTGGCTGGG AAGAAAGAAT TCACTTGGAT
    GGCCGAACGT TTTATATTGA TCATAGCAGC CAGGTGTTAT GTGGAGAAAA TGATACCAGA
    GAATCAGTGC CTTCATACGA TAGCAAAATT ACTCAGTGGG AAGACCCAAG ACTGCAGAAC
    CCAGCTATTA CTGGTCCGGC TGTCCCTTAC TCCAGAGAAT TTAAGCAGAA ATATGACTAC
    TTCAGGAAGA AATTAAAGAA ACCTGCTGAT ATCCCCAATA GGTTTGAAAT GAAACTTCAC
    AGAAATAACA TATTTGAAGA GTCCTATCGG AGAATTATGT CCGTGAAAAG ACCAGATGTC
    CTAAAAGCTA GACTGTGGAT TGAGTTTGAA TCAGAGAAAG GTCTTGACTA TGGGGGTGTG
    GCCAGAGAAT GGTTCTTCTT ACTGTCCAAA GAGATGTTCA ACCCCTACTA CGGCCTCTTT
    GAGTACTCTG CCACGGACAA CTACACCCTT CAGATCAACC CTAATTCAGG CCTCTGTAAT
    GAGGATCATT TGTCCTACTT CACTTTTATT GGAAGAGTTG CTGGTCTGGC CGTATTTCAT
    GGGAAGCTCT TAGATGGTTT CTTCATTAGA CCATTTTACA AGATGATGTT GGGAAAGCAG
    ATAACCCTGA ATGACATGGA ATCTGTGGAT AGTGAATATT ACAACTCTTT GAAATGGATC
    CTGGAGAATG ACCCTACTGA GCTGGACCTC ATGTTCTGCA TAGACGAAGA AAACTTTGGA
    CAGACATATC AAGTGGATTT GAAGCCCAAT GGGTCAGAAA TAATGGTCAC AAATGAAAAC
    AAAAGGGAAT ATATCGACTT AGTCATCCAG TGGAGATTTG TGAACAGGGT CCAGAAGCAG
    ATGAACGCCT TCTTGGAGGG ATTCACAGAA CTACTTCCTA TTGATTTGAT TAAAATTTTT
    GATGAAAATG AGCTGGAGTT GCTCATGTGC GGCCTCGGTG ATGTGGATGT GAATGACTGG
    AGACAGCATT CTATTTACAA GAACGGCTAC TGCCCAAACC ACCCCGTCAT TCAGTGGTTC
    TGGAAGGCTG TGCTACTCAT GGACGCCGAA AAGCGTATCC GGTTACTGCA GTTTGTCACA
    GGGACATCGC GAGTACCTAT GAATGGATTT GCCGAACTTT ATGGTTCCAA TGGTCCTCAG
    CTGTTTACAA TAGAGCAATG GGGCAGTCCT GAGAAACTGC CCAGAGCTCA CACATGCTTT
    AATCGCCTTG ACTTACCTCC ATATGAAACC TTTGAAGATT TACGAGAGAA ACTTCTCATG
    GCCGTGGAAA ATGCTCAAGG ATTTGAAGGG GTGGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006722493.1
    CDS586..3204
    Translation

    Target ORF information:

    RefSeq Version XM_006722430.4
    Organism Homo sapiens(human)
    Definition Homo sapiens NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006722430.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGCGAC CCTATACATT TAAGGACTTT CTCCTCAGAC CAAGAAGTCA TAAGTCTCGA 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCAA AAAATGGAGG TCAAGATGAA
    GAAAACAGTG ACCAGAGGGA TGACATGGAG CATGGATGGG AAGTTGTTGA CTCAAATGAC
    TCGGCTTCTC AGCACCAAGA GGAACTTCCT CCTCCTCCTC TGCCTCCCGG GTGGGAAGAA
    AAAGTGGACA ATTTAGGCCG AACTTACTAT GTCAACCACA ACAACCGGAC CACTCAGTGG
    CACAGACCAA GCCTGATGGA CGTGTCCTCG GAGTCGGACA ATAACATCAG ACAGATCAAC
    CAGGAGGCAG CACACCGGCG CTTCCGCTCC CGCAGGCACA TCAGCGAAGA CTTGGAGCCC
    GAGCCCTCGG AGGGCGGGGA TGTCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAT
    ATCGCTGGAG ACTCTCTCGG TCTGGCTCTG CCCCCACCAC CGGCCTCCCC AGGATCTCGG
    ACCAGCCCTC AGGAGCTGTC AGAGGAACTA AGCAGAAGGC TTCAGATCAC TCCAGACTCC
    AATGGGGAAC AGTTCAGCTC TTTGATTCAA AGAGAACCCT CCTCAAGGTT GAGGTCATGC
    AGTGTCACCG ACGCAGTTGC AGAACAGGGC CATCTACCAC CGCCCAGTGC CCCAGCTGGG
    AGAGCGCGTT CATCAACTGT CACGGGTGGT GAGGAACCAA CGCCATCAGT GGCCTATGTA
    CATACCACGC CGGGTCTGCC TTCAGGCTGG GAAGAAAGAA AAGATGCTAA GGGGCGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GACCTATCAT GCAGCTTGCA
    GAAGATGGTG CGTCCGGATC AGCCACAAAC AGTAACAACC ATCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CTGCCCCGCT GGAGGGTGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GACACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CTTGCCACCC
    GGCTGGGAAA TGAGGATAGC GCCAAACGGC CGGCCCTTCT TCATTGATCA TAACACAAAG
    ACTACAACCT GGGAAGATCC ACGTTTGAAA TTTCCAGTAC ATATGCGGTC AAAGACATCT
    TTAAACCCCA ATGACCTTGG CCCCCTTCCT CCTGGCTGGG AAGAAAGAAT TCACTTGGAT
    GGCCGAACGT TTTATATTGA TCATAGCAGC CAGGTGTTAT GTGGAGAAAA TGATACCAGA
    GAATCAGTGC CTTCATACGA TAGCAAAATT ACTCAGTGGG AAGACCCAAG ACTGCAGAAC
    CCAGCTATTA CTGGTCCGGC TGTCCCTTAC TCCAGAGAAT TTAAGCAGAA ATATGACTAC
    TTCAGGAAGA AATTAAAGAA ACCTGCTGAT ATCCCCAATA GGTTTGAAAT GAAACTTCAC
    AGAAATAACA TATTTGAAGA GTCCTATCGG AGAATTATGT CCGTGAAAAG ACCAGATGTC
    CTAAAAGCTA GACTGTGGAT TGAGTTTGAA TCAGAGAAAG GTCTTGACTA TGGGGGTGTG
    GCCAGAGAAT GGTTCTTCTT ACTGTCCAAA GAGATGTTCA ACCCCTACTA CGGCCTCTTT
    GAGTACTCTG CCACGGACAA CTACACCCTT CAGATCAACC CTAATTCAGG CCTCTGTAAT
    GAGGATCATT TGTCCTACTT CACTTTTATT GGAAGAGTTG CTGGTCTGGC CGTATTTCAT
    GGGAAGCTCT TAGATGGTTT CTTCATTAGA CCATTTTACA AGATGATGTT GGGAAAGCAG
    ATAACCCTGA ATGACATGGA ATCTGTGGAT AGTGAATATT ACAACTCTTT GAAATGGATC
    CTGGAGAATG ACCCTACTGA GCTGGACCTC ATGTTCTGCA TAGACGAAGA AAACTTTGGA
    CAGACATATC AAGTGGATTT GAAGCCCAAT GGGTCAGAAA TAATGGTCAC AAATGAAAAC
    AAAAGGGAAT ATATCGACTT AGTCATCCAG TGGAGATTTG TGAACAGGGT CCAGAAGCAG
    ATGAACGCCT TCTTGGAGGG ATTCACAGAA CTACTTCCTA TTGATTTGAT TAAAATTTTT
    GATGAAAATG AGCTGGAGTT GCTCATGTGC GGCCTCGGTG ATGTGGATGT GAATGACTGG
    AGACAGCATT CTATTTACAA GAACGGCTAC TGCCCAAACC ACCCCGTCAT TCAGTGGTTC
    TGGAAGGCTG TGCTACTCAT GGACGCCGAA AAGCGTATCC GGTTACTGCA GTTTGTCACA
    GGGACATCGC GAGTACCTAT GAATGGATTT GCCGAACTTT ATGGTTCCAA TGGTCCTCAG
    CTGTTTACAA TAGAGCAATG GGGCAGTCCT GAGAAACTGC CCAGAGCTCA CACATGCTTT
    AATCGCCTTG ACTTACCTCC ATATGAAACC TTTGAAGATT TACGAGAGAA ACTTCTCATG
    GCCGTGGAAA ATGCTCAAGG ATTTGAAGGG GTGGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu65973
    Clone ID Related Accession (Same CDS sequence) XM_005266658.4
    Accession Version XM_005266658.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2883bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005266658.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGCTCCTG AAATCCGCAG GACGAACTCC GCGGAGAGGA CTCCGCAGGG CCAGGGGTGC 
    ACATTTAAGA TCAGGCAGGA TCAGAACGCG GGGCAGCAGC ATTTTAGAAT TCTTGTAACC
    CGGGGGCCGG AAGAAGCTCC CCAGAATCCC GAGGAGAAAA GCGCCAAAAG CCCTGTTTCC
    ACGGGAGCTG ACACCACGAC ACGAGACGAC TTCCTGGGCC AGGTGGACGT GCCCCTTAGT
    CACCTTCCGA CAGAAGATCC AACCATGGAG CGACCCTATA CATTTAAGGA CTTTCTCCTC
    AGACCAAGAA GTCATAAGTC TCGAGTTAAG GGATTTTTGC GATTGAAAAT GGCCTATATG
    CCAAAAAATG GAGGTCAAGA TGAAGAAAAC AGTGACCAGA GGGATGACAT GGAGCATGGA
    TGGGAAGTTG TTGACTCAAA TGACTCGGCT TCTCAGCACC AAGAGGAACT TCCTCCTCCT
    CCTCTGCCTC CCGGGTGGGA AGAAAAAGTG GACAATTTAG GCCGAACTTA CTATGTCAAC
    CACAACAACC GGACCACTCA GTGGCACAGA CCAAGCCTGA TGGACGTGTC CTCGGAGTCG
    GACAATAACA TCAGACAGAT CAACCAGGAG GCAGCACACC GGCGCTTCCG CTCCCGCAGG
    CACATCAGCG AAGACTTGGA GCCCGAGCCC TCGGAGGGCG GGGATGTCCC CGAGCCTTGG
    GAGACCATTT CAGAGGAAGT GAATATCGCT GGAGACTCTC TCGGTCTGGC TCTGCCCCCA
    CCACCGGCCT CCCCAGGATC TCGGACCAGC CCTCAGGAGC TGTCAGAGGA ACTAAGCAGA
    AGGCTTCAGA TCACTCCAGA CTCCAATGGG GAACAGTTCA GCTCTTTGAT TCAAAGAGAA
    CCCTCCTCAA GGTTGAGGTC ATGCAGTGTC ACCGACGCAG TTGCAGAACA GGGCCATCTA
    CCACCGCCCA GTGCCCCAGC TGGGAGAGCG CGTTCATCAA CTGTCACGGG TGGTGAGGAA
    CCAACGCCAT CAGTGGCCTA TGTACATACC ACGCCGGGTC TGCCTTCAGG CTGGGAAGAA
    AGAAAAGATG CTAAGGGGCG CACATACTAT GTCAATCATA ACAATCGAAC CACAACTTGG
    ACTCGACCTA TCATGCAGCT TGCAGAAGAT GGTGCGTCCG GATCAGCCAC AAACAGTAAC
    AACCATCTAA TCGAGCCTCA GATCCGCCGG CCTCGTAGCC TCAGCTCGCC AACAGTAACT
    TTATCTGCCC CGCTGGAGGG TGCCAAGGAC TCACCCGTAC GTCGGGCTGT GAAAGACACC
    CTTTCCAACC CACAGTCCCC ACAGCCATCA CCTTACAACT CCCCCAAACC ACAACACAAA
    GTCACACAGA GCTTCTTGCC ACCCGGCTGG GAAATGAGGA TAGCGCCAAA CGGCCGGCCC
    TTCTTCATTG ATCATAACAC AAAGACTACA ACCTGGGAAG ATCCACGTTT GAAATTTCCA
    GTACATATGC GGTCAAAGAC ATCTTTAAAC CCCAATGACC TTGGCCCCCT TCCTCCTGGC
    TGGGAAGAAA GAATTCACTT GGATGGCCGA ACGTTTTATA TTGATCATAG CAGCCAGGTG
    TTATGTGGAG AAAATGATAC CAGAGAATCA GTGCCTTCAT ACGATAGCAA AATTACTCAG
    TGGGAAGACC CAAGACTGCA GAACCCAGCT ATTACTGGTC CGGCTGTCCC TTACTCCAGA
    GAATTTAAGC AGAAATATGA CTACTTCAGG AAGAAATTAA AGAAACCTGC TGATATCCCC
    AATAGGTTTG AAATGAAACT TCACAGAAAT AACATATTTG AAGAGTCCTA TCGGAGAATT
    ATGTCCGTGA AAAGACCAGA TGTCCTAAAA GCTAGACTGT GGATTGAGTT TGAATCAGAG
    AAAGGTCTTG ACTATGGGGG TGTGGCCAGA GAATGGTTCT TCTTACTGTC CAAAGAGATG
    TTCAACCCCT ACTACGGCCT CTTTGAGTAC TCTGCCACGG ACAACTACAC CCTTCAGATC
    AACCCTAATT CAGGCCTCTG TAATGAGGAT CATTTGTCCT ACTTCACTTT TATTGGAAGA
    GTTGCTGGTC TGGCCGTATT TCATGGGAAG CTCTTAGATG GTTTCTTCAT TAGACCATTT
    TACAAGATGA TGTTGGGAAA GCAGATAACC CTGAATGACA TGGAATCTGT GGATAGTGAA
    TATTACAACT CTTTGAAATG GATCCTGGAG AATGACCCTA CTGAGCTGGA CCTCATGTTC
    TGCATAGACG AAGAAAACTT TGGACAGACA TATCAAGTGG ATTTGAAGCC CAATGGGTCA
    GAAATAATGG TCACAAATGA AAACAAAAGG GAATATATCG ACTTAGTCAT CCAGTGGAGA
    TTTGTGAACA GGGTCCAGAA GCAGATGAAC GCCTTCTTGG AGGGATTCAC AGAACTACTT
    CCTATTGATT TGATTAAAAT TTTTGATGAA AATGAGCTGG AGTTGCTCAT GTGCGGCCTC
    GGTGATGTGG ATGTGAATGA CTGGAGACAG CATTCTATTT ACAAGAACGG CTACTGCCCA
    AACCACCCCG TCATTCAGTG GTTCTGGAAG GCTGTGCTAC TCATGGACGC CGAAAAGCGT
    ATCCGGTTAC TGCAGTTTGT CACAGGGACA TCGCGAGTAC CTATGAATGG ATTTGCCGAA
    CTTTATGGTT CCAATGGTCC TCAGCTGTTT ACAATAGAGC AATGGGGCAG TCCTGAGAAA
    CTGCCCAGAG CTCACACATG CTTTAATCGC CTTGACTTAC CTCCATATGA AACCTTTGAA
    GATTTACGAG AGAAACTTCT CATGGCCGTG GAAAATGCTC AAGGATTTGA AGGGGTGGAT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005266715.2
    CDS260..3142
    Translation

    Target ORF information:

    RefSeq Version XM_005266658.4
    Organism Homo sapiens(human)
    Definition Homo sapiens NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005266658.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGCTCCTG AAATCCGCAG GACGAACTCC GCGGAGAGGA CTCCGCAGGG CCAGGGGTGC 
    ACATTTAAGA TCAGGCAGGA TCAGAACGCG GGGCAGCAGC ATTTTAGAAT TCTTGTAACC
    CGGGGGCCGG AAGAAGCTCC CCAGAATCCC GAGGAGAAAA GCGCCAAAAG CCCTGTTTCC
    ACGGGAGCTG ACACCACGAC ACGAGACGAC TTCCTGGGCC AGGTGGACGT GCCCCTTAGT
    CACCTTCCGA CAGAAGATCC AACCATGGAG CGACCCTATA CATTTAAGGA CTTTCTCCTC
    AGACCAAGAA GTCATAAGTC TCGAGTTAAG GGATTTTTGC GATTGAAAAT GGCCTATATG
    CCAAAAAATG GAGGTCAAGA TGAAGAAAAC AGTGACCAGA GGGATGACAT GGAGCATGGA
    TGGGAAGTTG TTGACTCAAA TGACTCGGCT TCTCAGCACC AAGAGGAACT TCCTCCTCCT
    CCTCTGCCTC CCGGGTGGGA AGAAAAAGTG GACAATTTAG GCCGAACTTA CTATGTCAAC
    CACAACAACC GGACCACTCA GTGGCACAGA CCAAGCCTGA TGGACGTGTC CTCGGAGTCG
    GACAATAACA TCAGACAGAT CAACCAGGAG GCAGCACACC GGCGCTTCCG CTCCCGCAGG
    CACATCAGCG AAGACTTGGA GCCCGAGCCC TCGGAGGGCG GGGATGTCCC CGAGCCTTGG
    GAGACCATTT CAGAGGAAGT GAATATCGCT GGAGACTCTC TCGGTCTGGC TCTGCCCCCA
    CCACCGGCCT CCCCAGGATC TCGGACCAGC CCTCAGGAGC TGTCAGAGGA ACTAAGCAGA
    AGGCTTCAGA TCACTCCAGA CTCCAATGGG GAACAGTTCA GCTCTTTGAT TCAAAGAGAA
    CCCTCCTCAA GGTTGAGGTC ATGCAGTGTC ACCGACGCAG TTGCAGAACA GGGCCATCTA
    CCACCGCCCA GTGCCCCAGC TGGGAGAGCG CGTTCATCAA CTGTCACGGG TGGTGAGGAA
    CCAACGCCAT CAGTGGCCTA TGTACATACC ACGCCGGGTC TGCCTTCAGG CTGGGAAGAA
    AGAAAAGATG CTAAGGGGCG CACATACTAT GTCAATCATA ACAATCGAAC CACAACTTGG
    ACTCGACCTA TCATGCAGCT TGCAGAAGAT GGTGCGTCCG GATCAGCCAC AAACAGTAAC
    AACCATCTAA TCGAGCCTCA GATCCGCCGG CCTCGTAGCC TCAGCTCGCC AACAGTAACT
    TTATCTGCCC CGCTGGAGGG TGCCAAGGAC TCACCCGTAC GTCGGGCTGT GAAAGACACC
    CTTTCCAACC CACAGTCCCC ACAGCCATCA CCTTACAACT CCCCCAAACC ACAACACAAA
    GTCACACAGA GCTTCTTGCC ACCCGGCTGG GAAATGAGGA TAGCGCCAAA CGGCCGGCCC
    TTCTTCATTG ATCATAACAC AAAGACTACA ACCTGGGAAG ATCCACGTTT GAAATTTCCA
    GTACATATGC GGTCAAAGAC ATCTTTAAAC CCCAATGACC TTGGCCCCCT TCCTCCTGGC
    TGGGAAGAAA GAATTCACTT GGATGGCCGA ACGTTTTATA TTGATCATAG CAGCCAGGTG
    TTATGTGGAG AAAATGATAC CAGAGAATCA GTGCCTTCAT ACGATAGCAA AATTACTCAG
    TGGGAAGACC CAAGACTGCA GAACCCAGCT ATTACTGGTC CGGCTGTCCC TTACTCCAGA
    GAATTTAAGC AGAAATATGA CTACTTCAGG AAGAAATTAA AGAAACCTGC TGATATCCCC
    AATAGGTTTG AAATGAAACT TCACAGAAAT AACATATTTG AAGAGTCCTA TCGGAGAATT
    ATGTCCGTGA AAAGACCAGA TGTCCTAAAA GCTAGACTGT GGATTGAGTT TGAATCAGAG
    AAAGGTCTTG ACTATGGGGG TGTGGCCAGA GAATGGTTCT TCTTACTGTC CAAAGAGATG
    TTCAACCCCT ACTACGGCCT CTTTGAGTAC TCTGCCACGG ACAACTACAC CCTTCAGATC
    AACCCTAATT CAGGCCTCTG TAATGAGGAT CATTTGTCCT ACTTCACTTT TATTGGAAGA
    GTTGCTGGTC TGGCCGTATT TCATGGGAAG CTCTTAGATG GTTTCTTCAT TAGACCATTT
    TACAAGATGA TGTTGGGAAA GCAGATAACC CTGAATGACA TGGAATCTGT GGATAGTGAA
    TATTACAACT CTTTGAAATG GATCCTGGAG AATGACCCTA CTGAGCTGGA CCTCATGTTC
    TGCATAGACG AAGAAAACTT TGGACAGACA TATCAAGTGG ATTTGAAGCC CAATGGGTCA
    GAAATAATGG TCACAAATGA AAACAAAAGG GAATATATCG ACTTAGTCAT CCAGTGGAGA
    TTTGTGAACA GGGTCCAGAA GCAGATGAAC GCCTTCTTGG AGGGATTCAC AGAACTACTT
    CCTATTGATT TGATTAAAAT TTTTGATGAA AATGAGCTGG AGTTGCTCAT GTGCGGCCTC
    GGTGATGTGG ATGTGAATGA CTGGAGACAG CATTCTATTT ACAAGAACGG CTACTGCCCA
    AACCACCCCG TCATTCAGTG GTTCTGGAAG GCTGTGCTAC TCATGGACGC CGAAAAGCGT
    ATCCGGTTAC TGCAGTTTGT CACAGGGACA TCGCGAGTAC CTATGAATGG ATTTGCCGAA
    CTTTATGGTT CCAATGGTCC TCAGCTGTTT ACAATAGAGC AATGGGGCAG TCCTGAGAAA
    CTGCCCAGAG CTCACACATG CTTTAATCGC CTTGACTTAC CTCCATATGA AACCTTTGAA
    GATTTACGAG AGAAACTTCT CATGGCCGTG GAAAATGCTC AAGGATTTGA AGGGGTGGAT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu65974
    Clone ID Related Accession (Same CDS sequence) XM_005266660.4
    Accession Version XM_005266660.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2829bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005266660.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGCTCCTG AAATCCGCAG GACGAACTCC GCGGAGAGGA CTCCGCAGGG CCAGGGGTGC 
    ACATTTAAGA TCAGGCAGGA TCAGAACGCG GGGCAGCAGC ATTTTAGAAT TCTTGTAACC
    CGGGGGCCGG AAGAAGCTCC CCAGAATCCC GAGGAGAAAA GCGCCAAAAG CCCTGTTTCC
    ACGGGAGCTG ACACCACGAC ACGAGACGAC TTCCTGGGCC AGGTGGACGT GCCCCTTAGT
    CACCTTCCGA CAGAAGATCC AACCATGGAG CGACCCTATA CATTTAAGGA CTTTCTCCTC
    AGACCAAGAA GTCATAAGTC TCGAGTTAAG GGATTTTTGC GATTGAAAAT GGCCTATATG
    CCAAAAAATG GAGGTCAAGA TGAAGAAAAC AGTGACCAGA GGGATGACAT GGAGCATGGA
    TGGGAAGTTG TTGACTCAAA TGACTCGGCT TCTCAGCACC AAGAGGAACT TCCTCCTCCT
    CCTCTGCCTC CCGGGTGGGA AGAAAAAGTG GACAATTTAG GCCGAACTTA CTATGTCAAC
    CACAACAACC GGACCACTCA GTGGCACAGA CCAAGCCTGA TGGACGTGTC CTCGGAGTCG
    GACAATAACA TCAGACAGAT CAACCAGGAG GCAGCACACC GGCGCTTCCG CTCCCGCAGG
    CACATCAGCG AAGACTTGGA GCCCGAGCCC TCGGAGGGCG GGGATGTCCC CGAGCCTTGG
    GAGACCATTT CAGAGGAAGT GAATATCGCT GGAGACTCTC TCGGTCTGGC TCTGCCCCCA
    CCACCGGCCT CCCCAGGATC TCGGACCAGC CCTCAGGAGC TGTCAGAGGA ACTAAGCAGA
    AGGCTTCAGA TCACTCCAGA CTCCAATGGG GAACAGTTCA GCTCTTTGAT TCAAAGAGAA
    CCCTCCTCAA GGTTGAGGTC ATGCAGTGTC ACCGACGCAG TTGCAGAACA GGGCCATCTA
    CCACCGCCCA GTGCCCCAGC TGGGAGAGCG CGTTCATCAA CTGTCACGGG TGGTGAGGAA
    CCAACGCCAT CAGTGGCCTA TGTACATACC ACGCCGGGTC TGCCTTCAGG CTGGGAAGAA
    AGAAAAGATG CTAAGGGGCG CACATACTAT GTCAATCATA ACAATCGAAC CACAACTTGG
    ACTCGACCTA TCATGCAGCT TGCAGAAGAT GGTGCGTCCG GATCAGCCAC AAACAGTAAC
    AACCATCTAA TCGAGCCTCA GATCCGCCGG CCTCGTAGCC TCAGCTCGCC AACAGTAACT
    TTATCTGCCC CGCTGGAGGG TGCCAAGGAC TCACCCGTAC GTCGGGCTGT GAAAGACACC
    CTTTCCAACC CACAGTCCCC ACAGCCATCA CCTTACAACT CCCCCAAACC ACAACACAAA
    GTCACACAGA GCTTCTTGCC ACCCGGCTGG GAAATGAGGA TAGCGCCAAA CGGCCGGCCC
    TTCTTCATTG ATCATAACAC AAAGACTACA ACCTGGGAAG ATCCACGTTT GAAATTTCCA
    GTACATATGC GGTCAAAGAC ATCTTTAAAC CCCAATGACC TTGGCCCCCT TCCTCCTGGC
    TGGGAAGAAA GAATTCACTT GGATGGCCGA ACGTTTTATA TTGATCATAA TAGCAAAATT
    ACTCAGTGGG AAGACCCAAG ACTGCAGAAC CCAGCTATTA CTGGTCCGGC TGTCCCTTAC
    TCCAGAGAAT TTAAGCAGAA ATATGACTAC TTCAGGAAGA AATTAAAGAA ACCTGCTGAT
    ATCCCCAATA GGTTTGAAAT GAAACTTCAC AGAAATAACA TATTTGAAGA GTCCTATCGG
    AGAATTATGT CCGTGAAAAG ACCAGATGTC CTAAAAGCTA GACTGTGGAT TGAGTTTGAA
    TCAGAGAAAG GTCTTGACTA TGGGGGTGTG GCCAGAGAAT GGTTCTTCTT ACTGTCCAAA
    GAGATGTTCA ACCCCTACTA CGGCCTCTTT GAGTACTCTG CCACGGACAA CTACACCCTT
    CAGATCAACC CTAATTCAGG CCTCTGTAAT GAGGATCATT TGTCCTACTT CACTTTTATT
    GGAAGAGTTG CTGGTCTGGC CGTATTTCAT GGGAAGCTCT TAGATGGTTT CTTCATTAGA
    CCATTTTACA AGATGATGTT GGGAAAGCAG ATAACCCTGA ATGACATGGA ATCTGTGGAT
    AGTGAATATT ACAACTCTTT GAAATGGATC CTGGAGAATG ACCCTACTGA GCTGGACCTC
    ATGTTCTGCA TAGACGAAGA AAACTTTGGA CAGACATATC AAGTGGATTT GAAGCCCAAT
    GGGTCAGAAA TAATGGTCAC AAATGAAAAC AAAAGGGAAT ATATCGACTT AGTCATCCAG
    TGGAGATTTG TGAACAGGGT CCAGAAGCAG ATGAACGCCT TCTTGGAGGG ATTCACAGAA
    CTACTTCCTA TTGATTTGAT TAAAATTTTT GATGAAAATG AGCTGGAGTT GCTCATGTGC
    GGCCTCGGTG ATGTGGATGT GAATGACTGG AGACAGCATT CTATTTACAA GAACGGCTAC
    TGCCCAAACC ACCCCGTCAT TCAGTGGTTC TGGAAGGCTG TGCTACTCAT GGACGCCGAA
    AAGCGTATCC GGTTACTGCA GTTTGTCACA GGGACATCGC GAGTACCTAT GAATGGATTT
    GCCGAACTTT ATGGTTCCAA TGGTCCTCAG CTGTTTACAA TAGAGCAATG GGGCAGTCCT
    GAGAAACTGC CCAGAGCTCA CACATGCTTT AATCGCCTTG ACTTACCTCC ATATGAAACC
    TTTGAAGATT TACGAGAGAA ACTTCTCATG GCCGTGGAAA ATGCTCAAGG ATTTGAAGGG
    GTGGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005266717.2
    CDS258..3086
    Translation

    Target ORF information:

    RefSeq Version XM_005266660.4
    Organism Homo sapiens(human)
    Definition Homo sapiens NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005266660.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGCTCCTG AAATCCGCAG GACGAACTCC GCGGAGAGGA CTCCGCAGGG CCAGGGGTGC 
    ACATTTAAGA TCAGGCAGGA TCAGAACGCG GGGCAGCAGC ATTTTAGAAT TCTTGTAACC
    CGGGGGCCGG AAGAAGCTCC CCAGAATCCC GAGGAGAAAA GCGCCAAAAG CCCTGTTTCC
    ACGGGAGCTG ACACCACGAC ACGAGACGAC TTCCTGGGCC AGGTGGACGT GCCCCTTAGT
    CACCTTCCGA CAGAAGATCC AACCATGGAG CGACCCTATA CATTTAAGGA CTTTCTCCTC
    AGACCAAGAA GTCATAAGTC TCGAGTTAAG GGATTTTTGC GATTGAAAAT GGCCTATATG
    CCAAAAAATG GAGGTCAAGA TGAAGAAAAC AGTGACCAGA GGGATGACAT GGAGCATGGA
    TGGGAAGTTG TTGACTCAAA TGACTCGGCT TCTCAGCACC AAGAGGAACT TCCTCCTCCT
    CCTCTGCCTC CCGGGTGGGA AGAAAAAGTG GACAATTTAG GCCGAACTTA CTATGTCAAC
    CACAACAACC GGACCACTCA GTGGCACAGA CCAAGCCTGA TGGACGTGTC CTCGGAGTCG
    GACAATAACA TCAGACAGAT CAACCAGGAG GCAGCACACC GGCGCTTCCG CTCCCGCAGG
    CACATCAGCG AAGACTTGGA GCCCGAGCCC TCGGAGGGCG GGGATGTCCC CGAGCCTTGG
    GAGACCATTT CAGAGGAAGT GAATATCGCT GGAGACTCTC TCGGTCTGGC TCTGCCCCCA
    CCACCGGCCT CCCCAGGATC TCGGACCAGC CCTCAGGAGC TGTCAGAGGA ACTAAGCAGA
    AGGCTTCAGA TCACTCCAGA CTCCAATGGG GAACAGTTCA GCTCTTTGAT TCAAAGAGAA
    CCCTCCTCAA GGTTGAGGTC ATGCAGTGTC ACCGACGCAG TTGCAGAACA GGGCCATCTA
    CCACCGCCCA GTGCCCCAGC TGGGAGAGCG CGTTCATCAA CTGTCACGGG TGGTGAGGAA
    CCAACGCCAT CAGTGGCCTA TGTACATACC ACGCCGGGTC TGCCTTCAGG CTGGGAAGAA
    AGAAAAGATG CTAAGGGGCG CACATACTAT GTCAATCATA ACAATCGAAC CACAACTTGG
    ACTCGACCTA TCATGCAGCT TGCAGAAGAT GGTGCGTCCG GATCAGCCAC AAACAGTAAC
    AACCATCTAA TCGAGCCTCA GATCCGCCGG CCTCGTAGCC TCAGCTCGCC AACAGTAACT
    TTATCTGCCC CGCTGGAGGG TGCCAAGGAC TCACCCGTAC GTCGGGCTGT GAAAGACACC
    CTTTCCAACC CACAGTCCCC ACAGCCATCA CCTTACAACT CCCCCAAACC ACAACACAAA
    GTCACACAGA GCTTCTTGCC ACCCGGCTGG GAAATGAGGA TAGCGCCAAA CGGCCGGCCC
    TTCTTCATTG ATCATAACAC AAAGACTACA ACCTGGGAAG ATCCACGTTT GAAATTTCCA
    GTACATATGC GGTCAAAGAC ATCTTTAAAC CCCAATGACC TTGGCCCCCT TCCTCCTGGC
    TGGGAAGAAA GAATTCACTT GGATGGCCGA ACGTTTTATA TTGATCATAA TAGCAAAATT
    ACTCAGTGGG AAGACCCAAG ACTGCAGAAC CCAGCTATTA CTGGTCCGGC TGTCCCTTAC
    TCCAGAGAAT TTAAGCAGAA ATATGACTAC TTCAGGAAGA AATTAAAGAA ACCTGCTGAT
    ATCCCCAATA GGTTTGAAAT GAAACTTCAC AGAAATAACA TATTTGAAGA GTCCTATCGG
    AGAATTATGT CCGTGAAAAG ACCAGATGTC CTAAAAGCTA GACTGTGGAT TGAGTTTGAA
    TCAGAGAAAG GTCTTGACTA TGGGGGTGTG GCCAGAGAAT GGTTCTTCTT ACTGTCCAAA
    GAGATGTTCA ACCCCTACTA CGGCCTCTTT GAGTACTCTG CCACGGACAA CTACACCCTT
    CAGATCAACC CTAATTCAGG CCTCTGTAAT GAGGATCATT TGTCCTACTT CACTTTTATT
    GGAAGAGTTG CTGGTCTGGC CGTATTTCAT GGGAAGCTCT TAGATGGTTT CTTCATTAGA
    CCATTTTACA AGATGATGTT GGGAAAGCAG ATAACCCTGA ATGACATGGA ATCTGTGGAT
    AGTGAATATT ACAACTCTTT GAAATGGATC CTGGAGAATG ACCCTACTGA GCTGGACCTC
    ATGTTCTGCA TAGACGAAGA AAACTTTGGA CAGACATATC AAGTGGATTT GAAGCCCAAT
    GGGTCAGAAA TAATGGTCAC AAATGAAAAC AAAAGGGAAT ATATCGACTT AGTCATCCAG
    TGGAGATTTG TGAACAGGGT CCAGAAGCAG ATGAACGCCT TCTTGGAGGG ATTCACAGAA
    CTACTTCCTA TTGATTTGAT TAAAATTTTT GATGAAAATG AGCTGGAGTT GCTCATGTGC
    GGCCTCGGTG ATGTGGATGT GAATGACTGG AGACAGCATT CTATTTACAA GAACGGCTAC
    TGCCCAAACC ACCCCGTCAT TCAGTGGTTC TGGAAGGCTG TGCTACTCAT GGACGCCGAA
    AAGCGTATCC GGTTACTGCA GTTTGTCACA GGGACATCGC GAGTACCTAT GAATGGATTT
    GCCGAACTTT ATGGTTCCAA TGGTCCTCAG CTGTTTACAA TAGAGCAATG GGGCAGTCCT
    GAGAAACTGC CCAGAGCTCA CACATGCTTT AATCGCCTTG ACTTACCTCC ATATGAAACC
    TTTGAAGATT TACGAGAGAA ACTTCTCATG GCCGTGGAAA ATGCTCAAGG ATTTGAAGGG
    GTGGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu65975
    Clone ID Related Accession (Same CDS sequence) XM_006722421.3 , XM_006722421.4
    Accession Version XM_006722421.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2823bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006722421.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGCTCCTG AAATCCGCAG GACGAACTCC GCGGAGAGGA CTCCGCAGGG CCAGGGGTGC 
    ACATTTAAGA TCAGGCAGGA TCAGAACGCG GGGCAGCAGC ATTTTAGAAT TCTTGTAACC
    CGGGGGCCGG AAGAAGCTCC CCAGAATCCC GAGGAGAAAA GCGCCAAAAG CCCTGTTTCC
    ACGGGAGCTG ACACCACGAC ACGAGACGAC TTCCTGGGCC AGGTGGACGT GCCCCTTAGT
    CACCTTCCGA CAGAAGATCC AACCATGGAG CGACCCTATA CATTTAAGGA CTTTCTCCTC
    AGACCAAGAA GTCATAAGTC TCGAGTTAAG GGATTTTTGC GATTGAAAAT GGCCTATATG
    CCAAAAAATG GAGGTCAAGA TGAAGAAAAC AGTGACCAGA GGGATGACAT GGAGCATGGA
    TGGGAAGTTG TTGACTCAAA TGACTCGGCT TCTCAGCACC AAGAGGAACT TCCTCCTCCT
    CCTCTGCCTC CCGGGTGGGA AGAAAAAGTG GACAATTTAG GCCGAACTTA CTATGTCAAC
    CACAACAACC GGACCACTCA GTGGCACAGA CCAAGCCTGA TGGACGTGTC CTCGGAGTCG
    GACAATAACA TCAGACAGAT CAACCAGGAG GCAGCACACC GGCGCTTCCG CTCCCGCAGG
    CACATCAGCG AAGACTTGGA GCCCGAGCCC TCGGAGGGCG GGGATGTCCC CGAGCCTTGG
    GAGACCATTT CAGAGGAAGT GAATATCGCT GGAGACTCTC TCGGTCTGGC TCTGCCCCCA
    CCACCGGCCT CCCCAGGATC TCGGACCAGC CCTCAGGAGC TGTCAGAGGA ACTAAGCAGA
    AGGCTTCAGA TCACTCCAGA CTCCAATGGG GAACAGTTCA GCTCTTTGAT TCAAAGAGAA
    CCCTCCTCAA GGTTGAGGTC ATGCAGTGTC ACCGACGCAG TTGCAGAACA GGGCCATCTA
    CCACCGCCAT CAGTGGCCTA TGTACATACC ACGCCGGGTC TGCCTTCAGG CTGGGAAGAA
    AGAAAAGATG CTAAGGGGCG CACATACTAT GTCAATCATA ACAATCGAAC CACAACTTGG
    ACTCGACCTA TCATGCAGCT TGCAGAAGAT GGTGCGTCCG GATCAGCCAC AAACAGTAAC
    AACCATCTAA TCGAGCCTCA GATCCGCCGG CCTCGTAGCC TCAGCTCGCC AACAGTAACT
    TTATCTGCCC CGCTGGAGGG TGCCAAGGAC TCACCCGTAC GTCGGGCTGT GAAAGACACC
    CTTTCCAACC CACAGTCCCC ACAGCCATCA CCTTACAACT CCCCCAAACC ACAACACAAA
    GTCACACAGA GCTTCTTGCC ACCCGGCTGG GAAATGAGGA TAGCGCCAAA CGGCCGGCCC
    TTCTTCATTG ATCATAACAC AAAGACTACA ACCTGGGAAG ATCCACGTTT GAAATTTCCA
    GTACATATGC GGTCAAAGAC ATCTTTAAAC CCCAATGACC TTGGCCCCCT TCCTCCTGGC
    TGGGAAGAAA GAATTCACTT GGATGGCCGA ACGTTTTATA TTGATCATAG CAGCCAGGTG
    TTATGTGGAG AAAATGATAC CAGAGAATCA GTGCCTTCAT ACGATAGCAA AATTACTCAG
    TGGGAAGACC CAAGACTGCA GAACCCAGCT ATTACTGGTC CGGCTGTCCC TTACTCCAGA
    GAATTTAAGC AGAAATATGA CTACTTCAGG AAGAAATTAA AGAAACCTGC TGATATCCCC
    AATAGGTTTG AAATGAAACT TCACAGAAAT AACATATTTG AAGAGTCCTA TCGGAGAATT
    ATGTCCGTGA AAAGACCAGA TGTCCTAAAA GCTAGACTGT GGATTGAGTT TGAATCAGAG
    AAAGGTCTTG ACTATGGGGG TGTGGCCAGA GAATGGTTCT TCTTACTGTC CAAAGAGATG
    TTCAACCCCT ACTACGGCCT CTTTGAGTAC TCTGCCACGG ACAACTACAC CCTTCAGATC
    AACCCTAATT CAGGCCTCTG TAATGAGGAT CATTTGTCCT ACTTCACTTT TATTGGAAGA
    GTTGCTGGTC TGGCCGTATT TCATGGGAAG CTCTTAGATG GTTTCTTCAT TAGACCATTT
    TACAAGATGA TGTTGGGAAA GCAGATAACC CTGAATGACA TGGAATCTGT GGATAGTGAA
    TATTACAACT CTTTGAAATG GATCCTGGAG AATGACCCTA CTGAGCTGGA CCTCATGTTC
    TGCATAGACG AAGAAAACTT TGGACAGACA TATCAAGTGG ATTTGAAGCC CAATGGGTCA
    GAAATAATGG TCACAAATGA AAACAAAAGG GAATATATCG ACTTAGTCAT CCAGTGGAGA
    TTTGTGAACA GGGTCCAGAA GCAGATGAAC GCCTTCTTGG AGGGATTCAC AGAACTACTT
    CCTATTGATT TGATTAAAAT TTTTGATGAA AATGAGCTGG AGTTGCTCAT GTGCGGCCTC
    GGTGATGTGG ATGTGAATGA CTGGAGACAG CATTCTATTT ACAAGAACGG CTACTGCCCA
    AACCACCCCG TCATTCAGTG GTTCTGGAAG GCTGTGCTAC TCATGGACGC CGAAAAGCGT
    ATCCGGTTAC TGCAGTTTGT CACAGGGACA TCGCGAGTAC CTATGAATGG ATTTGCCGAA
    CTTTATGGTT CCAATGGTCC TCAGCTGTTT ACAATAGAGC AATGGGGCAG TCCTGAGAAA
    CTGCCCAGAG CTCACACATG CTTTAATCGC CTTGACTTAC CTCCATATGA AACCTTTGAA
    GATTTACGAG AGAAACTTCT CATGGCCGTG GAAAATGCTC AAGGATTTGA AGGGGTGGAT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006722484.2
    CDS259..3081
    Translation

    Target ORF information:

    RefSeq Version XM_006722421.3
    Organism Homo sapiens(human)
    Definition Homo sapiens neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006722421.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGCTCCTG AAATCCGCAG GACGAACTCC GCGGAGAGGA CTCCGCAGGG CCAGGGGTGC 
    ACATTTAAGA TCAGGCAGGA TCAGAACGCG GGGCAGCAGC ATTTTAGAAT TCTTGTAACC
    CGGGGGCCGG AAGAAGCTCC CCAGAATCCC GAGGAGAAAA GCGCCAAAAG CCCTGTTTCC
    ACGGGAGCTG ACACCACGAC ACGAGACGAC TTCCTGGGCC AGGTGGACGT GCCCCTTAGT
    CACCTTCCGA CAGAAGATCC AACCATGGAG CGACCCTATA CATTTAAGGA CTTTCTCCTC
    AGACCAAGAA GTCATAAGTC TCGAGTTAAG GGATTTTTGC GATTGAAAAT GGCCTATATG
    CCAAAAAATG GAGGTCAAGA TGAAGAAAAC AGTGACCAGA GGGATGACAT GGAGCATGGA
    TGGGAAGTTG TTGACTCAAA TGACTCGGCT TCTCAGCACC AAGAGGAACT TCCTCCTCCT
    CCTCTGCCTC CCGGGTGGGA AGAAAAAGTG GACAATTTAG GCCGAACTTA CTATGTCAAC
    CACAACAACC GGACCACTCA GTGGCACAGA CCAAGCCTGA TGGACGTGTC CTCGGAGTCG
    GACAATAACA TCAGACAGAT CAACCAGGAG GCAGCACACC GGCGCTTCCG CTCCCGCAGG
    CACATCAGCG AAGACTTGGA GCCCGAGCCC TCGGAGGGCG GGGATGTCCC CGAGCCTTGG
    GAGACCATTT CAGAGGAAGT GAATATCGCT GGAGACTCTC TCGGTCTGGC TCTGCCCCCA
    CCACCGGCCT CCCCAGGATC TCGGACCAGC CCTCAGGAGC TGTCAGAGGA ACTAAGCAGA
    AGGCTTCAGA TCACTCCAGA CTCCAATGGG GAACAGTTCA GCTCTTTGAT TCAAAGAGAA
    CCCTCCTCAA GGTTGAGGTC ATGCAGTGTC ACCGACGCAG TTGCAGAACA GGGCCATCTA
    CCACCGCCAT CAGTGGCCTA TGTACATACC ACGCCGGGTC TGCCTTCAGG CTGGGAAGAA
    AGAAAAGATG CTAAGGGGCG CACATACTAT GTCAATCATA ACAATCGAAC CACAACTTGG
    ACTCGACCTA TCATGCAGCT TGCAGAAGAT GGTGCGTCCG GATCAGCCAC AAACAGTAAC
    AACCATCTAA TCGAGCCTCA GATCCGCCGG CCTCGTAGCC TCAGCTCGCC AACAGTAACT
    TTATCTGCCC CGCTGGAGGG TGCCAAGGAC TCACCCGTAC GTCGGGCTGT GAAAGACACC
    CTTTCCAACC CACAGTCCCC ACAGCCATCA CCTTACAACT CCCCCAAACC ACAACACAAA
    GTCACACAGA GCTTCTTGCC ACCCGGCTGG GAAATGAGGA TAGCGCCAAA CGGCCGGCCC
    TTCTTCATTG ATCATAACAC AAAGACTACA ACCTGGGAAG ATCCACGTTT GAAATTTCCA
    GTACATATGC GGTCAAAGAC ATCTTTAAAC CCCAATGACC TTGGCCCCCT TCCTCCTGGC
    TGGGAAGAAA GAATTCACTT GGATGGCCGA ACGTTTTATA TTGATCATAG CAGCCAGGTG
    TTATGTGGAG AAAATGATAC CAGAGAATCA GTGCCTTCAT ACGATAGCAA AATTACTCAG
    TGGGAAGACC CAAGACTGCA GAACCCAGCT ATTACTGGTC CGGCTGTCCC TTACTCCAGA
    GAATTTAAGC AGAAATATGA CTACTTCAGG AAGAAATTAA AGAAACCTGC TGATATCCCC
    AATAGGTTTG AAATGAAACT TCACAGAAAT AACATATTTG AAGAGTCCTA TCGGAGAATT
    ATGTCCGTGA AAAGACCAGA TGTCCTAAAA GCTAGACTGT GGATTGAGTT TGAATCAGAG
    AAAGGTCTTG ACTATGGGGG TGTGGCCAGA GAATGGTTCT TCTTACTGTC CAAAGAGATG
    TTCAACCCCT ACTACGGCCT CTTTGAGTAC TCTGCCACGG ACAACTACAC CCTTCAGATC
    AACCCTAATT CAGGCCTCTG TAATGAGGAT CATTTGTCCT ACTTCACTTT TATTGGAAGA
    GTTGCTGGTC TGGCCGTATT TCATGGGAAG CTCTTAGATG GTTTCTTCAT TAGACCATTT
    TACAAGATGA TGTTGGGAAA GCAGATAACC CTGAATGACA TGGAATCTGT GGATAGTGAA
    TATTACAACT CTTTGAAATG GATCCTGGAG AATGACCCTA CTGAGCTGGA CCTCATGTTC
    TGCATAGACG AAGAAAACTT TGGACAGACA TATCAAGTGG ATTTGAAGCC CAATGGGTCA
    GAAATAATGG TCACAAATGA AAACAAAAGG GAATATATCG ACTTAGTCAT CCAGTGGAGA
    TTTGTGAACA GGGTCCAGAA GCAGATGAAC GCCTTCTTGG AGGGATTCAC AGAACTACTT
    CCTATTGATT TGATTAAAAT TTTTGATGAA AATGAGCTGG AGTTGCTCAT GTGCGGCCTC
    GGTGATGTGG ATGTGAATGA CTGGAGACAG CATTCTATTT ACAAGAACGG CTACTGCCCA
    AACCACCCCG TCATTCAGTG GTTCTGGAAG GCTGTGCTAC TCATGGACGC CGAAAAGCGT
    ATCCGGTTAC TGCAGTTTGT CACAGGGACA TCGCGAGTAC CTATGAATGG ATTTGCCGAA
    CTTTATGGTT CCAATGGTCC TCAGCTGTTT ACAATAGAGC AATGGGGCAG TCCTGAGAAA
    CTGCCCAGAG CTCACACATG CTTTAATCGC CTTGACTTAC CTCCATATGA AACCTTTGAA
    GATTTACGAG AGAAACTTCT CATGGCCGTG GAAAATGCTC AAGGATTTGA AGGGGTGGAT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu65975
    Clone ID Related Accession (Same CDS sequence) XM_006722421.3 , XM_006722421.4
    Accession Version XM_006722421.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2823bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_006722421.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGCTCCTG AAATCCGCAG GACGAACTCC GCGGAGAGGA CTCCGCAGGG CCAGGGGTGC 
    ACATTTAAGA TCAGGCAGGA TCAGAACGCG GGGCAGCAGC ATTTTAGAAT TCTTGTAACC
    CGGGGGCCGG AAGAAGCTCC CCAGAATCCC GAGGAGAAAA GCGCCAAAAG CCCTGTTTCC
    ACGGGAGCTG ACACCACGAC ACGAGACGAC TTCCTGGGCC AGGTGGACGT GCCCCTTAGT
    CACCTTCCGA CAGAAGATCC AACCATGGAG CGACCCTATA CATTTAAGGA CTTTCTCCTC
    AGACCAAGAA GTCATAAGTC TCGAGTTAAG GGATTTTTGC GATTGAAAAT GGCCTATATG
    CCAAAAAATG GAGGTCAAGA TGAAGAAAAC AGTGACCAGA GGGATGACAT GGAGCATGGA
    TGGGAAGTTG TTGACTCAAA TGACTCGGCT TCTCAGCACC AAGAGGAACT TCCTCCTCCT
    CCTCTGCCTC CCGGGTGGGA AGAAAAAGTG GACAATTTAG GCCGAACTTA CTATGTCAAC
    CACAACAACC GGACCACTCA GTGGCACAGA CCAAGCCTGA TGGACGTGTC CTCGGAGTCG
    GACAATAACA TCAGACAGAT CAACCAGGAG GCAGCACACC GGCGCTTCCG CTCCCGCAGG
    CACATCAGCG AAGACTTGGA GCCCGAGCCC TCGGAGGGCG GGGATGTCCC CGAGCCTTGG
    GAGACCATTT CAGAGGAAGT GAATATCGCT GGAGACTCTC TCGGTCTGGC TCTGCCCCCA
    CCACCGGCCT CCCCAGGATC TCGGACCAGC CCTCAGGAGC TGTCAGAGGA ACTAAGCAGA
    AGGCTTCAGA TCACTCCAGA CTCCAATGGG GAACAGTTCA GCTCTTTGAT TCAAAGAGAA
    CCCTCCTCAA GGTTGAGGTC ATGCAGTGTC ACCGACGCAG TTGCAGAACA GGGCCATCTA
    CCACCGCCAT CAGTGGCCTA TGTACATACC ACGCCGGGTC TGCCTTCAGG CTGGGAAGAA
    AGAAAAGATG CTAAGGGGCG CACATACTAT GTCAATCATA ACAATCGAAC CACAACTTGG
    ACTCGACCTA TCATGCAGCT TGCAGAAGAT GGTGCGTCCG GATCAGCCAC AAACAGTAAC
    AACCATCTAA TCGAGCCTCA GATCCGCCGG CCTCGTAGCC TCAGCTCGCC AACAGTAACT
    TTATCTGCCC CGCTGGAGGG TGCCAAGGAC TCACCCGTAC GTCGGGCTGT GAAAGACACC
    CTTTCCAACC CACAGTCCCC ACAGCCATCA CCTTACAACT CCCCCAAACC ACAACACAAA
    GTCACACAGA GCTTCTTGCC ACCCGGCTGG GAAATGAGGA TAGCGCCAAA CGGCCGGCCC
    TTCTTCATTG ATCATAACAC AAAGACTACA ACCTGGGAAG ATCCACGTTT GAAATTTCCA
    GTACATATGC GGTCAAAGAC ATCTTTAAAC CCCAATGACC TTGGCCCCCT TCCTCCTGGC
    TGGGAAGAAA GAATTCACTT GGATGGCCGA ACGTTTTATA TTGATCATAG CAGCCAGGTG
    TTATGTGGAG AAAATGATAC CAGAGAATCA GTGCCTTCAT ACGATAGCAA AATTACTCAG
    TGGGAAGACC CAAGACTGCA GAACCCAGCT ATTACTGGTC CGGCTGTCCC TTACTCCAGA
    GAATTTAAGC AGAAATATGA CTACTTCAGG AAGAAATTAA AGAAACCTGC TGATATCCCC
    AATAGGTTTG AAATGAAACT TCACAGAAAT AACATATTTG AAGAGTCCTA TCGGAGAATT
    ATGTCCGTGA AAAGACCAGA TGTCCTAAAA GCTAGACTGT GGATTGAGTT TGAATCAGAG
    AAAGGTCTTG ACTATGGGGG TGTGGCCAGA GAATGGTTCT TCTTACTGTC CAAAGAGATG
    TTCAACCCCT ACTACGGCCT CTTTGAGTAC TCTGCCACGG ACAACTACAC CCTTCAGATC
    AACCCTAATT CAGGCCTCTG TAATGAGGAT CATTTGTCCT ACTTCACTTT TATTGGAAGA
    GTTGCTGGTC TGGCCGTATT TCATGGGAAG CTCTTAGATG GTTTCTTCAT TAGACCATTT
    TACAAGATGA TGTTGGGAAA GCAGATAACC CTGAATGACA TGGAATCTGT GGATAGTGAA
    TATTACAACT CTTTGAAATG GATCCTGGAG AATGACCCTA CTGAGCTGGA CCTCATGTTC
    TGCATAGACG AAGAAAACTT TGGACAGACA TATCAAGTGG ATTTGAAGCC CAATGGGTCA
    GAAATAATGG TCACAAATGA AAACAAAAGG GAATATATCG ACTTAGTCAT CCAGTGGAGA
    TTTGTGAACA GGGTCCAGAA GCAGATGAAC GCCTTCTTGG AGGGATTCAC AGAACTACTT
    CCTATTGATT TGATTAAAAT TTTTGATGAA AATGAGCTGG AGTTGCTCAT GTGCGGCCTC
    GGTGATGTGG ATGTGAATGA CTGGAGACAG CATTCTATTT ACAAGAACGG CTACTGCCCA
    AACCACCCCG TCATTCAGTG GTTCTGGAAG GCTGTGCTAC TCATGGACGC CGAAAAGCGT
    ATCCGGTTAC TGCAGTTTGT CACAGGGACA TCGCGAGTAC CTATGAATGG ATTTGCCGAA
    CTTTATGGTT CCAATGGTCC TCAGCTGTTT ACAATAGAGC AATGGGGCAG TCCTGAGAAA
    CTGCCCAGAG CTCACACATG CTTTAATCGC CTTGACTTAC CTCCATATGA AACCTTTGAA
    GATTTACGAG AGAAACTTCT CATGGCCGTG GAAAATGCTC AAGGATTTGA AGGGGTGGAT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006722484.2
    CDS260..3082
    Translation

    Target ORF information:

    RefSeq Version XM_006722421.4
    Organism Homo sapiens(human)
    Definition Homo sapiens NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006722421.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGCTCCTG AAATCCGCAG GACGAACTCC GCGGAGAGGA CTCCGCAGGG CCAGGGGTGC 
    ACATTTAAGA TCAGGCAGGA TCAGAACGCG GGGCAGCAGC ATTTTAGAAT TCTTGTAACC
    CGGGGGCCGG AAGAAGCTCC CCAGAATCCC GAGGAGAAAA GCGCCAAAAG CCCTGTTTCC
    ACGGGAGCTG ACACCACGAC ACGAGACGAC TTCCTGGGCC AGGTGGACGT GCCCCTTAGT
    CACCTTCCGA CAGAAGATCC AACCATGGAG CGACCCTATA CATTTAAGGA CTTTCTCCTC
    AGACCAAGAA GTCATAAGTC TCGAGTTAAG GGATTTTTGC GATTGAAAAT GGCCTATATG
    CCAAAAAATG GAGGTCAAGA TGAAGAAAAC AGTGACCAGA GGGATGACAT GGAGCATGGA
    TGGGAAGTTG TTGACTCAAA TGACTCGGCT TCTCAGCACC AAGAGGAACT TCCTCCTCCT
    CCTCTGCCTC CCGGGTGGGA AGAAAAAGTG GACAATTTAG GCCGAACTTA CTATGTCAAC
    CACAACAACC GGACCACTCA GTGGCACAGA CCAAGCCTGA TGGACGTGTC CTCGGAGTCG
    GACAATAACA TCAGACAGAT CAACCAGGAG GCAGCACACC GGCGCTTCCG CTCCCGCAGG
    CACATCAGCG AAGACTTGGA GCCCGAGCCC TCGGAGGGCG GGGATGTCCC CGAGCCTTGG
    GAGACCATTT CAGAGGAAGT GAATATCGCT GGAGACTCTC TCGGTCTGGC TCTGCCCCCA
    CCACCGGCCT CCCCAGGATC TCGGACCAGC CCTCAGGAGC TGTCAGAGGA ACTAAGCAGA
    AGGCTTCAGA TCACTCCAGA CTCCAATGGG GAACAGTTCA GCTCTTTGAT TCAAAGAGAA
    CCCTCCTCAA GGTTGAGGTC ATGCAGTGTC ACCGACGCAG TTGCAGAACA GGGCCATCTA
    CCACCGCCAT CAGTGGCCTA TGTACATACC ACGCCGGGTC TGCCTTCAGG CTGGGAAGAA
    AGAAAAGATG CTAAGGGGCG CACATACTAT GTCAATCATA ACAATCGAAC CACAACTTGG
    ACTCGACCTA TCATGCAGCT TGCAGAAGAT GGTGCGTCCG GATCAGCCAC AAACAGTAAC
    AACCATCTAA TCGAGCCTCA GATCCGCCGG CCTCGTAGCC TCAGCTCGCC AACAGTAACT
    TTATCTGCCC CGCTGGAGGG TGCCAAGGAC TCACCCGTAC GTCGGGCTGT GAAAGACACC
    CTTTCCAACC CACAGTCCCC ACAGCCATCA CCTTACAACT CCCCCAAACC ACAACACAAA
    GTCACACAGA GCTTCTTGCC ACCCGGCTGG GAAATGAGGA TAGCGCCAAA CGGCCGGCCC
    TTCTTCATTG ATCATAACAC AAAGACTACA ACCTGGGAAG ATCCACGTTT GAAATTTCCA
    GTACATATGC GGTCAAAGAC ATCTTTAAAC CCCAATGACC TTGGCCCCCT TCCTCCTGGC
    TGGGAAGAAA GAATTCACTT GGATGGCCGA ACGTTTTATA TTGATCATAG CAGCCAGGTG
    TTATGTGGAG AAAATGATAC CAGAGAATCA GTGCCTTCAT ACGATAGCAA AATTACTCAG
    TGGGAAGACC CAAGACTGCA GAACCCAGCT ATTACTGGTC CGGCTGTCCC TTACTCCAGA
    GAATTTAAGC AGAAATATGA CTACTTCAGG AAGAAATTAA AGAAACCTGC TGATATCCCC
    AATAGGTTTG AAATGAAACT TCACAGAAAT AACATATTTG AAGAGTCCTA TCGGAGAATT
    ATGTCCGTGA AAAGACCAGA TGTCCTAAAA GCTAGACTGT GGATTGAGTT TGAATCAGAG
    AAAGGTCTTG ACTATGGGGG TGTGGCCAGA GAATGGTTCT TCTTACTGTC CAAAGAGATG
    TTCAACCCCT ACTACGGCCT CTTTGAGTAC TCTGCCACGG ACAACTACAC CCTTCAGATC
    AACCCTAATT CAGGCCTCTG TAATGAGGAT CATTTGTCCT ACTTCACTTT TATTGGAAGA
    GTTGCTGGTC TGGCCGTATT TCATGGGAAG CTCTTAGATG GTTTCTTCAT TAGACCATTT
    TACAAGATGA TGTTGGGAAA GCAGATAACC CTGAATGACA TGGAATCTGT GGATAGTGAA
    TATTACAACT CTTTGAAATG GATCCTGGAG AATGACCCTA CTGAGCTGGA CCTCATGTTC
    TGCATAGACG AAGAAAACTT TGGACAGACA TATCAAGTGG ATTTGAAGCC CAATGGGTCA
    GAAATAATGG TCACAAATGA AAACAAAAGG GAATATATCG ACTTAGTCAT CCAGTGGAGA
    TTTGTGAACA GGGTCCAGAA GCAGATGAAC GCCTTCTTGG AGGGATTCAC AGAACTACTT
    CCTATTGATT TGATTAAAAT TTTTGATGAA AATGAGCTGG AGTTGCTCAT GTGCGGCCTC
    GGTGATGTGG ATGTGAATGA CTGGAGACAG CATTCTATTT ACAAGAACGG CTACTGCCCA
    AACCACCCCG TCATTCAGTG GTTCTGGAAG GCTGTGCTAC TCATGGACGC CGAAAAGCGT
    ATCCGGTTAC TGCAGTTTGT CACAGGGACA TCGCGAGTAC CTATGAATGG ATTTGCCGAA
    CTTTATGGTT CCAATGGTCC TCAGCTGTTT ACAATAGAGC AATGGGGCAG TCCTGAGAAA
    CTGCCCAGAG CTCACACATG CTTTAATCGC CTTGACTTAC CTCCATATGA AACCTTTGAA
    GATTTACGAG AGAAACTTCT CATGGCCGTG GAAAATGCTC AAGGATTTGA AGGGGTGGAT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu65976
    Clone ID Related Accession (Same CDS sequence) XM_005266663.4
    Accession Version XM_005266663.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2769bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005266663.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCTCCTG AAATCCGCAG GACGAACTCC GCGGAGAGGA CTCCGCAGGG CCAGGGGTGC 
    ACATTTAAGA TCAGGCAGGA TCAGAACGCG GGGCAGCAGC ATTTTAGAAT TCTTGTAACC
    CGGGGGCCGG AAGAAGCTCC CCAGAATCCC GAGGAGAAAA GCGCCAAAAG CCCTGTTTCC
    ACGGGAGCTG ACACCACGAC ACGAGACGAC TTCCTGGGCC AGGTGGACGT GCCCCTTAGT
    CACCTTCCGA CAGAAGATCC AACCATGGAG CGACCCTATA CATTTAAGGA CTTTCTCCTC
    AGACCAAGAA GTCATAAGTC TCGAGTTAAG GGATTTTTGC GATTGAAAAT GGCCTATATG
    CCAAAAAATG GAGGTCAAGA TGAAGAAAAC AGTGACCAGA GGGATGACAT GGAGCATGGA
    TGGGAAGTTG TTGACTCAAA TGACTCGGCT TCTCAGCACC AAGAGGAACT TCCTCCTCCT
    CCTCTGCCTC CCGGGTGGGA AGAAAAAGTG GACAATTTAG GCCGAACTTA CTATGTCAAC
    CACAACAACC GGACCACTCA GTGGCACAGA CCAAGCCTGA TGGACGTGTC CTCGGAGTCG
    GACAATAACA TCAGACAGAT CAACCAGGAG GCAGCACACC GGCGCTTCCG CTCCCGCAGG
    CACATCAGCG AAGACTTGGA GCCCGAGCCC TCGGAGGGCG GGGATGTCCC CGAGCCTTGG
    GAGACCATTT CAGAGGAAGT GAATATCGCT GGAGACTCTC TCGGTCTGGC TCTGCCCCCA
    CCACCGGCCT CCCCAGGATC TCGGACCAGC CCTCAGGAGC TGTCAGAGGA ACTAAGCAGA
    AGGCTTCAGA TCACTCCAGA CTCCAATGGG GAACAGTTCA GCTCTTTGAT TCAAAGAGAA
    CCCTCCTCAA GGTTGAGGTC ATGCAGTGTC ACCGACGCAG TTGCAGAACA GGGCCATCTA
    CCACCGCCAT CAGTGGCCTA TGTACATACC ACGCCGGGTC TGCCTTCAGG CTGGGAAGAA
    AGAAAAGATG CTAAGGGGCG CACATACTAT GTCAATCATA ACAATCGAAC CACAACTTGG
    ACTCGACCTA TCATGCAGCT TGCAGAAGAT GGTGCGTCCG GATCAGCCAC AAACAGTAAC
    AACCATCTAA TCGAGCCTCA GATCCGCCGG CCTCGTAGCC TCAGCTCGCC AACAGTAACT
    TTATCTGCCC CGCTGGAGGG TGCCAAGGAC TCACCCGTAC GTCGGGCTGT GAAAGACACC
    CTTTCCAACC CACAGTCCCC ACAGCCATCA CCTTACAACT CCCCCAAACC ACAACACAAA
    GTCACACAGA GCTTCTTGCC ACCCGGCTGG GAAATGAGGA TAGCGCCAAA CGGCCGGCCC
    TTCTTCATTG ATCATAACAC AAAGACTACA ACCTGGGAAG ATCCACGTTT GAAATTTCCA
    GTACATATGC GGTCAAAGAC ATCTTTAAAC CCCAATGACC TTGGCCCCCT TCCTCCTGGC
    TGGGAAGAAA GAATTCACTT GGATGGCCGA ACGTTTTATA TTGATCATAA TAGCAAAATT
    ACTCAGTGGG AAGACCCAAG ACTGCAGAAC CCAGCTATTA CTGGTCCGGC TGTCCCTTAC
    TCCAGAGAAT TTAAGCAGAA ATATGACTAC TTCAGGAAGA AATTAAAGAA ACCTGCTGAT
    ATCCCCAATA GGTTTGAAAT GAAACTTCAC AGAAATAACA TATTTGAAGA GTCCTATCGG
    AGAATTATGT CCGTGAAAAG ACCAGATGTC CTAAAAGCTA GACTGTGGAT TGAGTTTGAA
    TCAGAGAAAG GTCTTGACTA TGGGGGTGTG GCCAGAGAAT GGTTCTTCTT ACTGTCCAAA
    GAGATGTTCA ACCCCTACTA CGGCCTCTTT GAGTACTCTG CCACGGACAA CTACACCCTT
    CAGATCAACC CTAATTCAGG CCTCTGTAAT GAGGATCATT TGTCCTACTT CACTTTTATT
    GGAAGAGTTG CTGGTCTGGC CGTATTTCAT GGGAAGCTCT TAGATGGTTT CTTCATTAGA
    CCATTTTACA AGATGATGTT GGGAAAGCAG ATAACCCTGA ATGACATGGA ATCTGTGGAT
    AGTGAATATT ACAACTCTTT GAAATGGATC CTGGAGAATG ACCCTACTGA GCTGGACCTC
    ATGTTCTGCA TAGACGAAGA AAACTTTGGA CAGACATATC AAGTGGATTT GAAGCCCAAT
    GGGTCAGAAA TAATGGTCAC AAATGAAAAC AAAAGGGAAT ATATCGACTT AGTCATCCAG
    TGGAGATTTG TGAACAGGGT CCAGAAGCAG ATGAACGCCT TCTTGGAGGG ATTCACAGAA
    CTACTTCCTA TTGATTTGAT TAAAATTTTT GATGAAAATG AGCTGGAGTT GCTCATGTGC
    GGCCTCGGTG ATGTGGATGT GAATGACTGG AGACAGCATT CTATTTACAA GAACGGCTAC
    TGCCCAAACC ACCCCGTCAT TCAGTGGTTC TGGAAGGCTG TGCTACTCAT GGACGCCGAA
    AAGCGTATCC GGTTACTGCA GTTTGTCACA GGGACATCGC GAGTACCTAT GAATGGATTT
    GCCGAACTTT ATGGTTCCAA TGGTCCTCAG CTGTTTACAA TAGAGCAATG GGGCAGTCCT
    GAGAAACTGC CCAGAGCTCA CACATGCTTT AATCGCCTTG ACTTACCTCC ATATGAAACC
    TTTGAAGATT TACGAGAGAA ACTTCTCATG GCCGTGGAAA ATGCTCAAGG ATTTGAAGGG
    GTGGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005266720.2
    CDS258..3026
    Translation

    Target ORF information:

    RefSeq Version XM_005266663.4
    Organism Homo sapiens(human)
    Definition Homo sapiens NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005266663.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCTCCTG AAATCCGCAG GACGAACTCC GCGGAGAGGA CTCCGCAGGG CCAGGGGTGC 
    ACATTTAAGA TCAGGCAGGA TCAGAACGCG GGGCAGCAGC ATTTTAGAAT TCTTGTAACC
    CGGGGGCCGG AAGAAGCTCC CCAGAATCCC GAGGAGAAAA GCGCCAAAAG CCCTGTTTCC
    ACGGGAGCTG ACACCACGAC ACGAGACGAC TTCCTGGGCC AGGTGGACGT GCCCCTTAGT
    CACCTTCCGA CAGAAGATCC AACCATGGAG CGACCCTATA CATTTAAGGA CTTTCTCCTC
    AGACCAAGAA GTCATAAGTC TCGAGTTAAG GGATTTTTGC GATTGAAAAT GGCCTATATG
    CCAAAAAATG GAGGTCAAGA TGAAGAAAAC AGTGACCAGA GGGATGACAT GGAGCATGGA
    TGGGAAGTTG TTGACTCAAA TGACTCGGCT TCTCAGCACC AAGAGGAACT TCCTCCTCCT
    CCTCTGCCTC CCGGGTGGGA AGAAAAAGTG GACAATTTAG GCCGAACTTA CTATGTCAAC
    CACAACAACC GGACCACTCA GTGGCACAGA CCAAGCCTGA TGGACGTGTC CTCGGAGTCG
    GACAATAACA TCAGACAGAT CAACCAGGAG GCAGCACACC GGCGCTTCCG CTCCCGCAGG
    CACATCAGCG AAGACTTGGA GCCCGAGCCC TCGGAGGGCG GGGATGTCCC CGAGCCTTGG
    GAGACCATTT CAGAGGAAGT GAATATCGCT GGAGACTCTC TCGGTCTGGC TCTGCCCCCA
    CCACCGGCCT CCCCAGGATC TCGGACCAGC CCTCAGGAGC TGTCAGAGGA ACTAAGCAGA
    AGGCTTCAGA TCACTCCAGA CTCCAATGGG GAACAGTTCA GCTCTTTGAT TCAAAGAGAA
    CCCTCCTCAA GGTTGAGGTC ATGCAGTGTC ACCGACGCAG TTGCAGAACA GGGCCATCTA
    CCACCGCCAT CAGTGGCCTA TGTACATACC ACGCCGGGTC TGCCTTCAGG CTGGGAAGAA
    AGAAAAGATG CTAAGGGGCG CACATACTAT GTCAATCATA ACAATCGAAC CACAACTTGG
    ACTCGACCTA TCATGCAGCT TGCAGAAGAT GGTGCGTCCG GATCAGCCAC AAACAGTAAC
    AACCATCTAA TCGAGCCTCA GATCCGCCGG CCTCGTAGCC TCAGCTCGCC AACAGTAACT
    TTATCTGCCC CGCTGGAGGG TGCCAAGGAC TCACCCGTAC GTCGGGCTGT GAAAGACACC
    CTTTCCAACC CACAGTCCCC ACAGCCATCA CCTTACAACT CCCCCAAACC ACAACACAAA
    GTCACACAGA GCTTCTTGCC ACCCGGCTGG GAAATGAGGA TAGCGCCAAA CGGCCGGCCC
    TTCTTCATTG ATCATAACAC AAAGACTACA ACCTGGGAAG ATCCACGTTT GAAATTTCCA
    GTACATATGC GGTCAAAGAC ATCTTTAAAC CCCAATGACC TTGGCCCCCT TCCTCCTGGC
    TGGGAAGAAA GAATTCACTT GGATGGCCGA ACGTTTTATA TTGATCATAA TAGCAAAATT
    ACTCAGTGGG AAGACCCAAG ACTGCAGAAC CCAGCTATTA CTGGTCCGGC TGTCCCTTAC
    TCCAGAGAAT TTAAGCAGAA ATATGACTAC TTCAGGAAGA AATTAAAGAA ACCTGCTGAT
    ATCCCCAATA GGTTTGAAAT GAAACTTCAC AGAAATAACA TATTTGAAGA GTCCTATCGG
    AGAATTATGT CCGTGAAAAG ACCAGATGTC CTAAAAGCTA GACTGTGGAT TGAGTTTGAA
    TCAGAGAAAG GTCTTGACTA TGGGGGTGTG GCCAGAGAAT GGTTCTTCTT ACTGTCCAAA
    GAGATGTTCA ACCCCTACTA CGGCCTCTTT GAGTACTCTG CCACGGACAA CTACACCCTT
    CAGATCAACC CTAATTCAGG CCTCTGTAAT GAGGATCATT TGTCCTACTT CACTTTTATT
    GGAAGAGTTG CTGGTCTGGC CGTATTTCAT GGGAAGCTCT TAGATGGTTT CTTCATTAGA
    CCATTTTACA AGATGATGTT GGGAAAGCAG ATAACCCTGA ATGACATGGA ATCTGTGGAT
    AGTGAATATT ACAACTCTTT GAAATGGATC CTGGAGAATG ACCCTACTGA GCTGGACCTC
    ATGTTCTGCA TAGACGAAGA AAACTTTGGA CAGACATATC AAGTGGATTT GAAGCCCAAT
    GGGTCAGAAA TAATGGTCAC AAATGAAAAC AAAAGGGAAT ATATCGACTT AGTCATCCAG
    TGGAGATTTG TGAACAGGGT CCAGAAGCAG ATGAACGCCT TCTTGGAGGG ATTCACAGAA
    CTACTTCCTA TTGATTTGAT TAAAATTTTT GATGAAAATG AGCTGGAGTT GCTCATGTGC
    GGCCTCGGTG ATGTGGATGT GAATGACTGG AGACAGCATT CTATTTACAA GAACGGCTAC
    TGCCCAAACC ACCCCGTCAT TCAGTGGTTC TGGAAGGCTG TGCTACTCAT GGACGCCGAA
    AAGCGTATCC GGTTACTGCA GTTTGTCACA GGGACATCGC GAGTACCTAT GAATGGATTT
    GCCGAACTTT ATGGTTCCAA TGGTCCTCAG CTGTTTACAA TAGAGCAATG GGGCAGTCCT
    GAGAAACTGC CCAGAGCTCA CACATGCTTT AATCGCCTTG ACTTACCTCC ATATGAAACC
    TTTGAAGATT TACGAGAGAA ACTTCTCATG GCCGTGGAAA ATGCTCAAGG ATTTGAAGGG
    GTGGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu65977
    Clone ID Related Accession (Same CDS sequence) XM_006722424.3
    Accession Version XM_006722424.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2373bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006722424.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGCTCCTG AAATCCGCAG GACGAACTCC GCGGAGAGGA CTCCGCAGGG CCAGGGGTGC 
    ACATTTAAGA TCAGGCAGGA TCAGAACGCG GGGCAGCAGC ATTTTAGAAT TCTTGTAACC
    CGGGGGCCGG AAGAAGCTCC CCAGAATCCC GAGGAGAAAA GCGCCAAAAG CCCTGTTTCC
    ACGGGAGCTG ACACCACGAC ACGAGACGAC TTCCTGGGCC AGGTGGACGT GCCCCTTAGT
    CACCTTCCGA CAGAAGATCC AACCATGGAG CGACCCTATA CATTTAAGGA CTTTCTCCTC
    AGACCAAGAA GTCATAAGTC TCGAGTTAAG GGATTTTTGC GATTGAAAAT GGCCTATATG
    CCAAAAAATG GAGGTCAAGA TGAAGAAAAC AGTGACCAGA GGGATGACAT GGAGCATGGA
    TGGGAAGTTG TTGACTCAAA TGACTCGGCT TCTCAGCACC AAGAGGAACT TCCTCCTCCT
    CCTCTGCCTC CCGGGTGGGA AGAAAAAGTG GACAATTTAG GCCGAACTTA CTATGTCAAC
    CACAACAACC GGACCACTCA GTGGCACAGA CCAAGCCTGA TGGACGTGTC CTCGGAGTCG
    GACAATAACA TCAGACAGAT CAACCAGGAG GCAGCACACC GGCGCTTCCG CTCCCGCAGG
    CACATCAGCG AAGACTTGGA GCCCGAGCCC TCGGAGGGCG GGGATGTCCC CGAGCCTTGG
    GAGACCATTT CAGAGGAAGT GAATATCGCT GGAGACTCTC TCGGTCTGGC TCTGCCCCCA
    CCACCGGCCT CCCCAGGATC TCGGACCAGC CCTCAGGAGC TGTCAGAGGA ACTAAGCAGA
    AGGCTTCAGA TCACTCCAGA CTCCAATGGG GAACAGTTCA GCTCTTTGAT TCAAAGAGAA
    CCCTCCTCAA GGTTGAGGTC ATGCAGTGTC ACCGACGCAG TTGCAGAACA GGGCCATCTA
    CCACCGGAAG ATCCACGTTT GAAATTTCCA GTACATATGC GGTCAAAGAC ATCTTTAAAC
    CCCAATGACC TTGGCCCCCT TCCTCCTGGC TGGGAAGAAA GAATTCACTT GGATGGCCGA
    ACGTTTTATA TTGATCATAG CAGCCAGGTG TTATGTGGAG AAAATGATAC CAGAGAATCA
    GTGCCTTCAT ACGATAGCAA AATTACTCAG TGGGAAGACC CAAGACTGCA GAACCCAGCT
    ATTACTGGTC CGGCTGTCCC TTACTCCAGA GAATTTAAGC AGAAATATGA CTACTTCAGG
    AAGAAATTAA AGAAACCTGC TGATATCCCC AATAGGTTTG AAATGAAACT TCACAGAAAT
    AACATATTTG AAGAGTCCTA TCGGAGAATT ATGTCCGTGA AAAGACCAGA TGTCCTAAAA
    GCTAGACTGT GGATTGAGTT TGAATCAGAG AAAGGTCTTG ACTATGGGGG TGTGGCCAGA
    GAATGGTTCT TCTTACTGTC CAAAGAGATG TTCAACCCCT ACTACGGCCT CTTTGAGTAC
    TCTGCCACGG ACAACTACAC CCTTCAGATC AACCCTAATT CAGGCCTCTG TAATGAGGAT
    CATTTGTCCT ACTTCACTTT TATTGGAAGA GTTGCTGGTC TGGCCGTATT TCATGGGAAG
    CTCTTAGATG GTTTCTTCAT TAGACCATTT TACAAGATGA TGTTGGGAAA GCAGATAACC
    CTGAATGACA TGGAATCTGT GGATAGTGAA TATTACAACT CTTTGAAATG GATCCTGGAG
    AATGACCCTA CTGAGCTGGA CCTCATGTTC TGCATAGACG AAGAAAACTT TGGACAGACA
    TATCAAGTGG ATTTGAAGCC CAATGGGTCA GAAATAATGG TCACAAATGA AAACAAAAGG
    GAATATATCG ACTTAGTCAT CCAGTGGAGA TTTGTGAACA GGGTCCAGAA GCAGATGAAC
    GCCTTCTTGG AGGGATTCAC AGAACTACTT CCTATTGATT TGATTAAAAT TTTTGATGAA
    AATGAGCTGG AGTTGCTCAT GTGCGGCCTC GGTGATGTGG ATGTGAATGA CTGGAGACAG
    CATTCTATTT ACAAGAACGG CTACTGCCCA AACCACCCCG TCATTCAGTG GTTCTGGAAG
    GCTGTGCTAC TCATGGACGC CGAAAAGCGT ATCCGGTTAC TGCAGTTTGT CACAGGGACA
    TCGCGAGTAC CTATGAATGG ATTTGCCGAA CTTTATGGTT CCAATGGTCC TCAGCTGTTT
    ACAATAGAGC AATGGGGCAG TCCTGAGAAA CTGCCCAGAG CTCACACATG CTTTAATCGC
    CTTGACTTAC CTCCATATGA AACCTTTGAA GATTTACGAG AGAAACTTCT CATGGCCGTG
    GAAAATGCTC AAGGATTTGA AGGGGTGGAT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006722487.2
    CDS262..2634
    Translation

    Target ORF information:

    RefSeq Version XM_006722424.3
    Organism Homo sapiens(human)
    Definition Homo sapiens NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006722424.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGCTCCTG AAATCCGCAG GACGAACTCC GCGGAGAGGA CTCCGCAGGG CCAGGGGTGC 
    ACATTTAAGA TCAGGCAGGA TCAGAACGCG GGGCAGCAGC ATTTTAGAAT TCTTGTAACC
    CGGGGGCCGG AAGAAGCTCC CCAGAATCCC GAGGAGAAAA GCGCCAAAAG CCCTGTTTCC
    ACGGGAGCTG ACACCACGAC ACGAGACGAC TTCCTGGGCC AGGTGGACGT GCCCCTTAGT
    CACCTTCCGA CAGAAGATCC AACCATGGAG CGACCCTATA CATTTAAGGA CTTTCTCCTC
    AGACCAAGAA GTCATAAGTC TCGAGTTAAG GGATTTTTGC GATTGAAAAT GGCCTATATG
    CCAAAAAATG GAGGTCAAGA TGAAGAAAAC AGTGACCAGA GGGATGACAT GGAGCATGGA
    TGGGAAGTTG TTGACTCAAA TGACTCGGCT TCTCAGCACC AAGAGGAACT TCCTCCTCCT
    CCTCTGCCTC CCGGGTGGGA AGAAAAAGTG GACAATTTAG GCCGAACTTA CTATGTCAAC
    CACAACAACC GGACCACTCA GTGGCACAGA CCAAGCCTGA TGGACGTGTC CTCGGAGTCG
    GACAATAACA TCAGACAGAT CAACCAGGAG GCAGCACACC GGCGCTTCCG CTCCCGCAGG
    CACATCAGCG AAGACTTGGA GCCCGAGCCC TCGGAGGGCG GGGATGTCCC CGAGCCTTGG
    GAGACCATTT CAGAGGAAGT GAATATCGCT GGAGACTCTC TCGGTCTGGC TCTGCCCCCA
    CCACCGGCCT CCCCAGGATC TCGGACCAGC CCTCAGGAGC TGTCAGAGGA ACTAAGCAGA
    AGGCTTCAGA TCACTCCAGA CTCCAATGGG GAACAGTTCA GCTCTTTGAT TCAAAGAGAA
    CCCTCCTCAA GGTTGAGGTC ATGCAGTGTC ACCGACGCAG TTGCAGAACA GGGCCATCTA
    CCACCGGAAG ATCCACGTTT GAAATTTCCA GTACATATGC GGTCAAAGAC ATCTTTAAAC
    CCCAATGACC TTGGCCCCCT TCCTCCTGGC TGGGAAGAAA GAATTCACTT GGATGGCCGA
    ACGTTTTATA TTGATCATAG CAGCCAGGTG TTATGTGGAG AAAATGATAC CAGAGAATCA
    GTGCCTTCAT ACGATAGCAA AATTACTCAG TGGGAAGACC CAAGACTGCA GAACCCAGCT
    ATTACTGGTC CGGCTGTCCC TTACTCCAGA GAATTTAAGC AGAAATATGA CTACTTCAGG
    AAGAAATTAA AGAAACCTGC TGATATCCCC AATAGGTTTG AAATGAAACT TCACAGAAAT
    AACATATTTG AAGAGTCCTA TCGGAGAATT ATGTCCGTGA AAAGACCAGA TGTCCTAAAA
    GCTAGACTGT GGATTGAGTT TGAATCAGAG AAAGGTCTTG ACTATGGGGG TGTGGCCAGA
    GAATGGTTCT TCTTACTGTC CAAAGAGATG TTCAACCCCT ACTACGGCCT CTTTGAGTAC
    TCTGCCACGG ACAACTACAC CCTTCAGATC AACCCTAATT CAGGCCTCTG TAATGAGGAT
    CATTTGTCCT ACTTCACTTT TATTGGAAGA GTTGCTGGTC TGGCCGTATT TCATGGGAAG
    CTCTTAGATG GTTTCTTCAT TAGACCATTT TACAAGATGA TGTTGGGAAA GCAGATAACC
    CTGAATGACA TGGAATCTGT GGATAGTGAA TATTACAACT CTTTGAAATG GATCCTGGAG
    AATGACCCTA CTGAGCTGGA CCTCATGTTC TGCATAGACG AAGAAAACTT TGGACAGACA
    TATCAAGTGG ATTTGAAGCC CAATGGGTCA GAAATAATGG TCACAAATGA AAACAAAAGG
    GAATATATCG ACTTAGTCAT CCAGTGGAGA TTTGTGAACA GGGTCCAGAA GCAGATGAAC
    GCCTTCTTGG AGGGATTCAC AGAACTACTT CCTATTGATT TGATTAAAAT TTTTGATGAA
    AATGAGCTGG AGTTGCTCAT GTGCGGCCTC GGTGATGTGG ATGTGAATGA CTGGAGACAG
    CATTCTATTT ACAAGAACGG CTACTGCCCA AACCACCCCG TCATTCAGTG GTTCTGGAAG
    GCTGTGCTAC TCATGGACGC CGAAAAGCGT ATCCGGTTAC TGCAGTTTGT CACAGGGACA
    TCGCGAGTAC CTATGAATGG ATTTGCCGAA CTTTATGGTT CCAATGGTCC TCAGCTGTTT
    ACAATAGAGC AATGGGGCAG TCCTGAGAAA CTGCCCAGAG CTCACACATG CTTTAATCGC
    CTTGACTTAC CTCCATATGA AACCTTTGAA GATTTACGAG AGAAACTTCT CATGGCCGTG
    GAAAATGCTC AAGGATTTGA AGGGGTGGAT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu65978
    Clone ID Related Accession (Same CDS sequence) XM_006722425.3
    Accession Version XM_006722425.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2319bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006722425.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCTCCTG AAATCCGCAG GACGAACTCC GCGGAGAGGA CTCCGCAGGG CCAGGGGTGC 
    ACATTTAAGA TCAGGCAGGA TCAGAACGCG GGGCAGCAGC ATTTTAGAAT TCTTGTAACC
    CGGGGGCCGG AAGAAGCTCC CCAGAATCCC GAGGAGAAAA GCGCCAAAAG CCCTGTTTCC
    ACGGGAGCTG ACACCACGAC ACGAGACGAC TTCCTGGGCC AGGTGGACGT GCCCCTTAGT
    CACCTTCCGA CAGAAGATCC AACCATGGAG CGACCCTATA CATTTAAGGA CTTTCTCCTC
    AGACCAAGAA GTCATAAGTC TCGAGTTAAG GGATTTTTGC GATTGAAAAT GGCCTATATG
    CCAAAAAATG GAGGTCAAGA TGAAGAAAAC AGTGACCAGA GGGATGACAT GGAGCATGGA
    TGGGAAGTTG TTGACTCAAA TGACTCGGCT TCTCAGCACC AAGAGGAACT TCCTCCTCCT
    CCTCTGCCTC CCGGGTGGGA AGAAAAAGTG GACAATTTAG GCCGAACTTA CTATGTCAAC
    CACAACAACC GGACCACTCA GTGGCACAGA CCAAGCCTGA TGGACGTGTC CTCGGAGTCG
    GACAATAACA TCAGACAGAT CAACCAGGAG GCAGCACACC GGCGCTTCCG CTCCCGCAGG
    CACATCAGCG AAGACTTGGA GCCCGAGCCC TCGGAGGGCG GGGATGTCCC CGAGCCTTGG
    GAGACCATTT CAGAGGAAGT GAATATCGCT GGAGACTCTC TCGGTCTGGC TCTGCCCCCA
    CCACCGGCCT CCCCAGGATC TCGGACCAGC CCTCAGGAGC TGTCAGAGGA ACTAAGCAGA
    AGGCTTCAGA TCACTCCAGA CTCCAATGGG GAACAGTTCA GCTCTTTGAT TCAAAGAGAA
    CCCTCCTCAA GGTTGAGGTC ATGCAGTGTC ACCGACGCAG TTGCAGAACA GGGCCATCTA
    CCACCGGAAG ATCCACGTTT GAAATTTCCA GTACATATGC GGTCAAAGAC ATCTTTAAAC
    CCCAATGACC TTGGCCCCCT TCCTCCTGGC TGGGAAGAAA GAATTCACTT GGATGGCCGA
    ACGTTTTATA TTGATCATAA TAGCAAAATT ACTCAGTGGG AAGACCCAAG ACTGCAGAAC
    CCAGCTATTA CTGGTCCGGC TGTCCCTTAC TCCAGAGAAT TTAAGCAGAA ATATGACTAC
    TTCAGGAAGA AATTAAAGAA ACCTGCTGAT ATCCCCAATA GGTTTGAAAT GAAACTTCAC
    AGAAATAACA TATTTGAAGA GTCCTATCGG AGAATTATGT CCGTGAAAAG ACCAGATGTC
    CTAAAAGCTA GACTGTGGAT TGAGTTTGAA TCAGAGAAAG GTCTTGACTA TGGGGGTGTG
    GCCAGAGAAT GGTTCTTCTT ACTGTCCAAA GAGATGTTCA ACCCCTACTA CGGCCTCTTT
    GAGTACTCTG CCACGGACAA CTACACCCTT CAGATCAACC CTAATTCAGG CCTCTGTAAT
    GAGGATCATT TGTCCTACTT CACTTTTATT GGAAGAGTTG CTGGTCTGGC CGTATTTCAT
    GGGAAGCTCT TAGATGGTTT CTTCATTAGA CCATTTTACA AGATGATGTT GGGAAAGCAG
    ATAACCCTGA ATGACATGGA ATCTGTGGAT AGTGAATATT ACAACTCTTT GAAATGGATC
    CTGGAGAATG ACCCTACTGA GCTGGACCTC ATGTTCTGCA TAGACGAAGA AAACTTTGGA
    CAGACATATC AAGTGGATTT GAAGCCCAAT GGGTCAGAAA TAATGGTCAC AAATGAAAAC
    AAAAGGGAAT ATATCGACTT AGTCATCCAG TGGAGATTTG TGAACAGGGT CCAGAAGCAG
    ATGAACGCCT TCTTGGAGGG ATTCACAGAA CTACTTCCTA TTGATTTGAT TAAAATTTTT
    GATGAAAATG AGCTGGAGTT GCTCATGTGC GGCCTCGGTG ATGTGGATGT GAATGACTGG
    AGACAGCATT CTATTTACAA GAACGGCTAC TGCCCAAACC ACCCCGTCAT TCAGTGGTTC
    TGGAAGGCTG TGCTACTCAT GGACGCCGAA AAGCGTATCC GGTTACTGCA GTTTGTCACA
    GGGACATCGC GAGTACCTAT GAATGGATTT GCCGAACTTT ATGGTTCCAA TGGTCCTCAG
    CTGTTTACAA TAGAGCAATG GGGCAGTCCT GAGAAACTGC CCAGAGCTCA CACATGCTTT
    AATCGCCTTG ACTTACCTCC ATATGAAACC TTTGAAGATT TACGAGAGAA ACTTCTCATG
    GCCGTGGAAA ATGCTCAAGG ATTTGAAGGG GTGGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006722488.2
    CDS260..2578
    Translation

    Target ORF information:

    RefSeq Version XM_006722425.3
    Organism Homo sapiens(human)
    Definition Homo sapiens NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006722425.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCTCCTG AAATCCGCAG GACGAACTCC GCGGAGAGGA CTCCGCAGGG CCAGGGGTGC 
    ACATTTAAGA TCAGGCAGGA TCAGAACGCG GGGCAGCAGC ATTTTAGAAT TCTTGTAACC
    CGGGGGCCGG AAGAAGCTCC CCAGAATCCC GAGGAGAAAA GCGCCAAAAG CCCTGTTTCC
    ACGGGAGCTG ACACCACGAC ACGAGACGAC TTCCTGGGCC AGGTGGACGT GCCCCTTAGT
    CACCTTCCGA CAGAAGATCC AACCATGGAG CGACCCTATA CATTTAAGGA CTTTCTCCTC
    AGACCAAGAA GTCATAAGTC TCGAGTTAAG GGATTTTTGC GATTGAAAAT GGCCTATATG
    CCAAAAAATG GAGGTCAAGA TGAAGAAAAC AGTGACCAGA GGGATGACAT GGAGCATGGA
    TGGGAAGTTG TTGACTCAAA TGACTCGGCT TCTCAGCACC AAGAGGAACT TCCTCCTCCT
    CCTCTGCCTC CCGGGTGGGA AGAAAAAGTG GACAATTTAG GCCGAACTTA CTATGTCAAC
    CACAACAACC GGACCACTCA GTGGCACAGA CCAAGCCTGA TGGACGTGTC CTCGGAGTCG
    GACAATAACA TCAGACAGAT CAACCAGGAG GCAGCACACC GGCGCTTCCG CTCCCGCAGG
    CACATCAGCG AAGACTTGGA GCCCGAGCCC TCGGAGGGCG GGGATGTCCC CGAGCCTTGG
    GAGACCATTT CAGAGGAAGT GAATATCGCT GGAGACTCTC TCGGTCTGGC TCTGCCCCCA
    CCACCGGCCT CCCCAGGATC TCGGACCAGC CCTCAGGAGC TGTCAGAGGA ACTAAGCAGA
    AGGCTTCAGA TCACTCCAGA CTCCAATGGG GAACAGTTCA GCTCTTTGAT TCAAAGAGAA
    CCCTCCTCAA GGTTGAGGTC ATGCAGTGTC ACCGACGCAG TTGCAGAACA GGGCCATCTA
    CCACCGGAAG ATCCACGTTT GAAATTTCCA GTACATATGC GGTCAAAGAC ATCTTTAAAC
    CCCAATGACC TTGGCCCCCT TCCTCCTGGC TGGGAAGAAA GAATTCACTT GGATGGCCGA
    ACGTTTTATA TTGATCATAA TAGCAAAATT ACTCAGTGGG AAGACCCAAG ACTGCAGAAC
    CCAGCTATTA CTGGTCCGGC TGTCCCTTAC TCCAGAGAAT TTAAGCAGAA ATATGACTAC
    TTCAGGAAGA AATTAAAGAA ACCTGCTGAT ATCCCCAATA GGTTTGAAAT GAAACTTCAC
    AGAAATAACA TATTTGAAGA GTCCTATCGG AGAATTATGT CCGTGAAAAG ACCAGATGTC
    CTAAAAGCTA GACTGTGGAT TGAGTTTGAA TCAGAGAAAG GTCTTGACTA TGGGGGTGTG
    GCCAGAGAAT GGTTCTTCTT ACTGTCCAAA GAGATGTTCA ACCCCTACTA CGGCCTCTTT
    GAGTACTCTG CCACGGACAA CTACACCCTT CAGATCAACC CTAATTCAGG CCTCTGTAAT
    GAGGATCATT TGTCCTACTT CACTTTTATT GGAAGAGTTG CTGGTCTGGC CGTATTTCAT
    GGGAAGCTCT TAGATGGTTT CTTCATTAGA CCATTTTACA AGATGATGTT GGGAAAGCAG
    ATAACCCTGA ATGACATGGA ATCTGTGGAT AGTGAATATT ACAACTCTTT GAAATGGATC
    CTGGAGAATG ACCCTACTGA GCTGGACCTC ATGTTCTGCA TAGACGAAGA AAACTTTGGA
    CAGACATATC AAGTGGATTT GAAGCCCAAT GGGTCAGAAA TAATGGTCAC AAATGAAAAC
    AAAAGGGAAT ATATCGACTT AGTCATCCAG TGGAGATTTG TGAACAGGGT CCAGAAGCAG
    ATGAACGCCT TCTTGGAGGG ATTCACAGAA CTACTTCCTA TTGATTTGAT TAAAATTTTT
    GATGAAAATG AGCTGGAGTT GCTCATGTGC GGCCTCGGTG ATGTGGATGT GAATGACTGG
    AGACAGCATT CTATTTACAA GAACGGCTAC TGCCCAAACC ACCCCGTCAT TCAGTGGTTC
    TGGAAGGCTG TGCTACTCAT GGACGCCGAA AAGCGTATCC GGTTACTGCA GTTTGTCACA
    GGGACATCGC GAGTACCTAT GAATGGATTT GCCGAACTTT ATGGTTCCAA TGGTCCTCAG
    CTGTTTACAA TAGAGCAATG GGGCAGTCCT GAGAAACTGC CCAGAGCTCA CACATGCTTT
    AATCGCCTTG ACTTACCTCC ATATGAAACC TTTGAAGATT TACGAGAGAA ACTTCTCATG
    GCCGTGGAAA ATGCTCAAGG ATTTGAAGGG GTGGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu104534
    Clone ID Related Accession (Same CDS sequence) XM_024451130.1
    Accession Version XM_024451130.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2559bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAGCGAC CCTATACATT TAAGGACTTT CTCCTCAGAC CAAGAAGTCA TAAGTCTCGA 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCAA AAAATGGAGG TCAAGATGAA
    GAAAACAGTG ACCAGAGGGA TGACATGGAG CATGGATGGG AAGTTGTTGA CTCAAATGAC
    TCGGCTTCTC AGCACCAAGA GGAACTTCCT CCTCCTCCTC TGCCTCCCGG GTGGGAAGAA
    AAAGTGGACA ATTTAGGCCG AACTTACTAT GTCAACCACA ACAACCGGAC CACTCAGTGG
    CACAGACCAA GCCTGATGGA CGTGTCCTCG GAGTCGGACA ATAACATCAG ACAGATCAAC
    CAGGAGGCAG CACACCGGCG CTTCCGCTCC CGCAGGCACA TCAGCGAAGA CTTGGAGCCC
    GAGCCCTCGG AGGGCGGGGA TGTCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAT
    ATCGCTGGAG ACTCTCTCGG TCTGGCTCTG CCCCCACCAC CGGCCTCCCC AGGATCTCGG
    ACCAGCCCTC AGGAGCTGTC AGAGGAACTA AGCAGAAGGC TTCAGATCAC TCCAGACTCC
    AATGGGGAAC AGTTCAGCTC TTTGATTCAA AGAGAACCCT CCTCAAGGTT GAGGTCATGC
    AGTGTCACCG ACGCAGTTGC AGAACAGGGC CATCTACCAC CGCCATCAGT GGCCTATGTA
    CATACCACGC CGGGTCTGCC TTCAGGCTGG GAAGAAAGAA AAGATGCTAA GGGGCGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GACCTATCAT GCAGCTTGCA
    GAAGATGGTG CGTCCGGATC AGCCACAAAC AGTAACAACC ATCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CTGCCCCGCT GGAGGGTGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GACACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CTTGCCACCC
    GGCTGGGAAA TGAGGATAGC GCCAAACGGC CGGCCCTTCT TCATTGATCA TAACACAAAG
    ACTACAACCT GGGAAGATCC ACGTTTGAAA TTTCCAGTAC ATATGCGGTC AAAGACATCT
    TTAAACCCCA ATGACCTTGG CCCCCTTCCT CCTGGCTGGG AAGAAAGAAT TCACTTGGAT
    GGCCGAACGT TTTATATTGA TCATAGCAGC CAGGTGTTAT GTGGAGAAAA TGATACCAGA
    GAATCAGTGC CTTCATACGA TAGCAAAATT ACTCAGTGGG AAGACCCAAG ACTGCAGAAC
    CCAGCTATTA CTGGTCCGGC TGTCCCTTAC TCCAGAGAAT TTAAGCAGAA ATATGACTAC
    TTCAGGAAGA AATTAAAGAA ACCTGCTGAT ATCCCCAATA GGTTTGAAAT GAAACTTCAC
    AGAAATAACA TATTTGAAGA GTCCTATCGG AGAATTATGT CCGTGAAAAG ACCAGATGTC
    CTAAAAGCTA GACTGTGGAT TGAGTTTGAA TCAGAGAAAG GTCTTGACTA TGGGGGTGTG
    GCCAGAGAAT GGTTCTTCTT ACTGTCCAAA GAGATGTTCA ACCCCTACTA CGGCCTCTTT
    GAGTACTCTG CCACGGACAA CTACACCCTT CAGATCAACC CTAATTCAGG CCTCTGTAAT
    GAGGATCATT TGTCCTACTT CACTTTTATT GGAAGAGTTG CTGGTCTGGC CGTATTTCAT
    GGGAAGCTCT TAGATGGTTT CTTCATTAGA CCATTTTACA AGATGATGTT GGGAAAGCAG
    ATAACCCTGA ATGACATGGA ATCTGTGGAT AGTGAATATT ACAACTCTTT GAAATGGATC
    CTGGAGAATG ACCCTACTGA GCTGGACCTC ATGTTCTGCA TAGACGAAGA AAACTTTGGA
    CAGACATATC AAGTGGATTT GAAGCCCAAT GGGTCAGAAA TAATGGTCAC AAATGAAAAC
    AAAAGGGAAT ATATCGACTT AGTCATCCAG TGGAGATTTG TGAACAGGGT CCAGAAGCAG
    ATGAACGCCT TCTTGGAGGG ATTCACAGAA CTACTTCCTA TTGATTTGAT TAAAATTTTT
    GATGAAAATG AGCTGGAGTT GCTCATGTGC GGCCTCGGTG ATGTGGATGT GAATGACTGG
    AGACAGCATT CTATTTACAA GAACGGCTAC TGCCCAAACC ACCCCGTCAT TCAGTGGTTC
    TGGAAGGCTG TGCTACTCAT GGACGCCGAA AAGCGTATCC GGTTACTGCA GTTTGTCACA
    GGGACATCGC GAGTACCTAT GAATGGATTT GCCGAACTTT ATGGTTCCAA TGGTCCTCAG
    CTGTTTACAA TAGAGCAATG GGGCAGTCCT GAGAAACTGC CCAGAGCTCA CACATGCTTT
    AATCGCCTTG ACTTACCTCC ATATGAAACC TTTGAAGATT TACGAGAGAA ACTTCTCATG
    GCCGTGGAAA ATGCTCAAGG ATTTGAAGGG GTGGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024306898.1
    CDS320..2878
    Translation

    Target ORF information:

    RefSeq Version XM_024451130.1
    Organism Homo sapiens(human)
    Definition Homo sapiens NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024451130.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAGCGAC CCTATACATT TAAGGACTTT CTCCTCAGAC CAAGAAGTCA TAAGTCTCGA 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCAA AAAATGGAGG TCAAGATGAA
    GAAAACAGTG ACCAGAGGGA TGACATGGAG CATGGATGGG AAGTTGTTGA CTCAAATGAC
    TCGGCTTCTC AGCACCAAGA GGAACTTCCT CCTCCTCCTC TGCCTCCCGG GTGGGAAGAA
    AAAGTGGACA ATTTAGGCCG AACTTACTAT GTCAACCACA ACAACCGGAC CACTCAGTGG
    CACAGACCAA GCCTGATGGA CGTGTCCTCG GAGTCGGACA ATAACATCAG ACAGATCAAC
    CAGGAGGCAG CACACCGGCG CTTCCGCTCC CGCAGGCACA TCAGCGAAGA CTTGGAGCCC
    GAGCCCTCGG AGGGCGGGGA TGTCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAT
    ATCGCTGGAG ACTCTCTCGG TCTGGCTCTG CCCCCACCAC CGGCCTCCCC AGGATCTCGG
    ACCAGCCCTC AGGAGCTGTC AGAGGAACTA AGCAGAAGGC TTCAGATCAC TCCAGACTCC
    AATGGGGAAC AGTTCAGCTC TTTGATTCAA AGAGAACCCT CCTCAAGGTT GAGGTCATGC
    AGTGTCACCG ACGCAGTTGC AGAACAGGGC CATCTACCAC CGCCATCAGT GGCCTATGTA
    CATACCACGC CGGGTCTGCC TTCAGGCTGG GAAGAAAGAA AAGATGCTAA GGGGCGCACA
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GACCTATCAT GCAGCTTGCA
    GAAGATGGTG CGTCCGGATC AGCCACAAAC AGTAACAACC ATCTAATCGA GCCTCAGATC
    CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CTGCCCCGCT GGAGGGTGCC
    AAGGACTCAC CCGTACGTCG GGCTGTGAAA GACACCCTTT CCAACCCACA GTCCCCACAG
    CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CTTGCCACCC
    GGCTGGGAAA TGAGGATAGC GCCAAACGGC CGGCCCTTCT TCATTGATCA TAACACAAAG
    ACTACAACCT GGGAAGATCC ACGTTTGAAA TTTCCAGTAC ATATGCGGTC AAAGACATCT
    TTAAACCCCA ATGACCTTGG CCCCCTTCCT CCTGGCTGGG AAGAAAGAAT TCACTTGGAT
    GGCCGAACGT TTTATATTGA TCATAGCAGC CAGGTGTTAT GTGGAGAAAA TGATACCAGA
    GAATCAGTGC CTTCATACGA TAGCAAAATT ACTCAGTGGG AAGACCCAAG ACTGCAGAAC
    CCAGCTATTA CTGGTCCGGC TGTCCCTTAC TCCAGAGAAT TTAAGCAGAA ATATGACTAC
    TTCAGGAAGA AATTAAAGAA ACCTGCTGAT ATCCCCAATA GGTTTGAAAT GAAACTTCAC
    AGAAATAACA TATTTGAAGA GTCCTATCGG AGAATTATGT CCGTGAAAAG ACCAGATGTC
    CTAAAAGCTA GACTGTGGAT TGAGTTTGAA TCAGAGAAAG GTCTTGACTA TGGGGGTGTG
    GCCAGAGAAT GGTTCTTCTT ACTGTCCAAA GAGATGTTCA ACCCCTACTA CGGCCTCTTT
    GAGTACTCTG CCACGGACAA CTACACCCTT CAGATCAACC CTAATTCAGG CCTCTGTAAT
    GAGGATCATT TGTCCTACTT CACTTTTATT GGAAGAGTTG CTGGTCTGGC CGTATTTCAT
    GGGAAGCTCT TAGATGGTTT CTTCATTAGA CCATTTTACA AGATGATGTT GGGAAAGCAG
    ATAACCCTGA ATGACATGGA ATCTGTGGAT AGTGAATATT ACAACTCTTT GAAATGGATC
    CTGGAGAATG ACCCTACTGA GCTGGACCTC ATGTTCTGCA TAGACGAAGA AAACTTTGGA
    CAGACATATC AAGTGGATTT GAAGCCCAAT GGGTCAGAAA TAATGGTCAC AAATGAAAAC
    AAAAGGGAAT ATATCGACTT AGTCATCCAG TGGAGATTTG TGAACAGGGT CCAGAAGCAG
    ATGAACGCCT TCTTGGAGGG ATTCACAGAA CTACTTCCTA TTGATTTGAT TAAAATTTTT
    GATGAAAATG AGCTGGAGTT GCTCATGTGC GGCCTCGGTG ATGTGGATGT GAATGACTGG
    AGACAGCATT CTATTTACAA GAACGGCTAC TGCCCAAACC ACCCCGTCAT TCAGTGGTTC
    TGGAAGGCTG TGCTACTCAT GGACGCCGAA AAGCGTATCC GGTTACTGCA GTTTGTCACA
    GGGACATCGC GAGTACCTAT GAATGGATTT GCCGAACTTT ATGGTTCCAA TGGTCCTCAG
    CTGTTTACAA TAGAGCAATG GGGCAGTCCT GAGAAACTGC CCAGAGCTCA CACATGCTTT
    AATCGCCTTG ACTTACCTCC ATATGAAACC TTTGAAGATT TACGAGAGAA ACTTCTCATG
    GCCGTGGAAA ATGCTCAAGG ATTTGAAGGG GTGGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu87831
    Clone ID Related Accession (Same CDS sequence) XM_017025676.1
    Accession Version XM_017025676.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2571bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGCTCCTG AAATCCGCAG GACGAACTCC GCGGAGAGGA CTCCGCAGGG CCAGGGGTGC 
    ACATTTAAGA TCAGGCAGGA TCAGAACGCG GGGCAGCAGC ATTTTAGAAT TCTTGTAACC
    CGGGGGCCGG AAGAAGCTCC CCAGAATCCC GAGGAGAAAA GCGCCAAAAG CCCTGTTTCC
    ACGGGAGCTG ACACCACGAC ACGAGACGAC TTCCTGGGCC AGGTGGACGT GCCCCTTAGT
    CACCTTCCGA CAGAAGATCC AACCATGGAG CGACCCTATA CATTTAAGGA CTTTCTCCTC
    AGACCAAGAA GTCATAAGTC TCGAGTTAAG GGATTTTTGC GATTGAAAAT GGCCTATATG
    CCAAAAAATG GAGGTCAAGA TGAAGAAAAC AGTGACCAGA GGGATGACAT GGAGCATGGA
    TGGGAAGTTG TTGACTCAAA TGACTCGGCT TCTCAGCACC AAGAGGAACT TCCTCCTCCT
    CCTCTGCCTC CCGGGTGGGA AGAAAAAGTG GACAATTTAG GCCGAACTTA CTATGTCAAC
    CACAACAACC GGACCACTCA GTGGCACAGA CCAAGCCTGA TGGACGTGTC CTCGGAGTCG
    GACAATAACA TCAGACAGAT CAACCAGGAG GCAGCACACC GGCGCTTCCG CTCCCGCAGG
    CACATCAGCG AAGACTTGGA GCCCGAGCCC TCGGAGGGCG GGGATGTCCC CGAGCCTTGG
    GAGACCATTT CAGAGGAAGT GAATATCGCT GGAGACTCTC TCGGTCTGGC TCTGCCCCCA
    CCACCGGCCT CCCCAGGATC TCGGACCAGC CCTCAGGAGC TGTCAGAGGA ACTAAGCAGA
    AGGCTTCAGA TCACTCCAGA CTCCAATGGG GAACAGTTCA GCTCTTTGAT TCAAAGAGAA
    CCCTCCTCAA GGTTGAGGTC ATGCAGTGTC ACCGACGCAG TTGCAGAACA GGGCCATCTA
    CCACCGGGTG CCAAGGACTC ACCCGTACGT CGGGCTGTGA AAGACACCCT TTCCAACCCA
    CAGTCCCCAC AGCCATCACC TTACAACTCC CCCAAACCAC AACACAAAGT CACACAGAGC
    TTCTTGCCAC CCGGCTGGGA AATGAGGATA GCGCCAAACG GCCGGCCCTT CTTCATTGAT
    CATAACACAA AGACTACAAC CTGGGAAGAT CCACGTTTGA AATTTCCAGT ACATATGCGG
    TCAAAGACAT CTTTAAACCC CAATGACCTT GGCCCCCTTC CTCCTGGCTG GGAAGAAAGA
    ATTCACTTGG ATGGCCGAAC GTTTTATATT GATCATAGCA GCCAGGTGTT ATGTGGAGAA
    AATGATACCA GAGAATCAGT GCCTTCATAC GATAGCAAAA TTACTCAGTG GGAAGACCCA
    AGACTGCAGA ACCCAGCTAT TACTGGTCCG GCTGTCCCTT ACTCCAGAGA ATTTAAGCAG
    AAATATGACT ACTTCAGGAA GAAATTAAAG AAACCTGCTG ATATCCCCAA TAGGTTTGAA
    ATGAAACTTC ACAGAAATAA CATATTTGAA GAGTCCTATC GGAGAATTAT GTCCGTGAAA
    AGACCAGATG TCCTAAAAGC TAGACTGTGG ATTGAGTTTG AATCAGAGAA AGGTCTTGAC
    TATGGGGGTG TGGCCAGAGA ATGGTTCTTC TTACTGTCCA AAGAGATGTT CAACCCCTAC
    TACGGCCTCT TTGAGTACTC TGCCACGGAC AACTACACCC TTCAGATCAA CCCTAATTCA
    GGCCTCTGTA ATGAGGATCA TTTGTCCTAC TTCACTTTTA TTGGAAGAGT TGCTGGTCTG
    GCCGTATTTC ATGGGAAGCT CTTAGATGGT TTCTTCATTA GACCATTTTA CAAGATGATG
    TTGGGAAAGC AGATAACCCT GAATGACATG GAATCTGTGG ATAGTGAATA TTACAACTCT
    TTGAAATGGA TCCTGGAGAA TGACCCTACT GAGCTGGACC TCATGTTCTG CATAGACGAA
    GAAAACTTTG GACAGACATA TCAAGTGGAT TTGAAGCCCA ATGGGTCAGA AATAATGGTC
    ACAAATGAAA ACAAAAGGGA ATATATCGAC TTAGTCATCC AGTGGAGATT TGTGAACAGG
    GTCCAGAAGC AGATGAACGC CTTCTTGGAG GGATTCACAG AACTACTTCC TATTGATTTG
    ATTAAAATTT TTGATGAAAA TGAGCTGGAG TTGCTCATGT GCGGCCTCGG TGATGTGGAT
    GTGAATGACT GGAGACAGCA TTCTATTTAC AAGAACGGCT ACTGCCCAAA CCACCCCGTC
    ATTCAGTGGT TCTGGAAGGC TGTGCTACTC ATGGACGCCG AAAAGCGTAT CCGGTTACTG
    CAGTTTGTCA CAGGGACATC GCGAGTACCT ATGAATGGAT TTGCCGAACT TTATGGTTCC
    AATGGTCCTC AGCTGTTTAC AATAGAGCAA TGGGGCAGTC CTGAGAAACT GCCCAGAGCT
    CACACATGCT TTAATCGCCT TGACTTACCT CCATATGAAA CCTTTGAAGA TTTACGAGAG
    AAACTTCTCA TGGCCGTGGA AAATGCTCAA GGATTTGAAG GGGTGGATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016881165.1
    CDS261..2831
    Translation

    Target ORF information:

    RefSeq Version XM_017025676.1
    Organism Homo sapiens(human)
    Definition Homo sapiens NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017025676.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGCTCCTG AAATCCGCAG GACGAACTCC GCGGAGAGGA CTCCGCAGGG CCAGGGGTGC 
    ACATTTAAGA TCAGGCAGGA TCAGAACGCG GGGCAGCAGC ATTTTAGAAT TCTTGTAACC
    CGGGGGCCGG AAGAAGCTCC CCAGAATCCC GAGGAGAAAA GCGCCAAAAG CCCTGTTTCC
    ACGGGAGCTG ACACCACGAC ACGAGACGAC TTCCTGGGCC AGGTGGACGT GCCCCTTAGT
    CACCTTCCGA CAGAAGATCC AACCATGGAG CGACCCTATA CATTTAAGGA CTTTCTCCTC
    AGACCAAGAA GTCATAAGTC TCGAGTTAAG GGATTTTTGC GATTGAAAAT GGCCTATATG
    CCAAAAAATG GAGGTCAAGA TGAAGAAAAC AGTGACCAGA GGGATGACAT GGAGCATGGA
    TGGGAAGTTG TTGACTCAAA TGACTCGGCT TCTCAGCACC AAGAGGAACT TCCTCCTCCT
    CCTCTGCCTC CCGGGTGGGA AGAAAAAGTG GACAATTTAG GCCGAACTTA CTATGTCAAC
    CACAACAACC GGACCACTCA GTGGCACAGA CCAAGCCTGA TGGACGTGTC CTCGGAGTCG
    GACAATAACA TCAGACAGAT CAACCAGGAG GCAGCACACC GGCGCTTCCG CTCCCGCAGG
    CACATCAGCG AAGACTTGGA GCCCGAGCCC TCGGAGGGCG GGGATGTCCC CGAGCCTTGG
    GAGACCATTT CAGAGGAAGT GAATATCGCT GGAGACTCTC TCGGTCTGGC TCTGCCCCCA
    CCACCGGCCT CCCCAGGATC TCGGACCAGC CCTCAGGAGC TGTCAGAGGA ACTAAGCAGA
    AGGCTTCAGA TCACTCCAGA CTCCAATGGG GAACAGTTCA GCTCTTTGAT TCAAAGAGAA
    CCCTCCTCAA GGTTGAGGTC ATGCAGTGTC ACCGACGCAG TTGCAGAACA GGGCCATCTA
    CCACCGGGTG CCAAGGACTC ACCCGTACGT CGGGCTGTGA AAGACACCCT TTCCAACCCA
    CAGTCCCCAC AGCCATCACC TTACAACTCC CCCAAACCAC AACACAAAGT CACACAGAGC
    TTCTTGCCAC CCGGCTGGGA AATGAGGATA GCGCCAAACG GCCGGCCCTT CTTCATTGAT
    CATAACACAA AGACTACAAC CTGGGAAGAT CCACGTTTGA AATTTCCAGT ACATATGCGG
    TCAAAGACAT CTTTAAACCC CAATGACCTT GGCCCCCTTC CTCCTGGCTG GGAAGAAAGA
    ATTCACTTGG ATGGCCGAAC GTTTTATATT GATCATAGCA GCCAGGTGTT ATGTGGAGAA
    AATGATACCA GAGAATCAGT GCCTTCATAC GATAGCAAAA TTACTCAGTG GGAAGACCCA
    AGACTGCAGA ACCCAGCTAT TACTGGTCCG GCTGTCCCTT ACTCCAGAGA ATTTAAGCAG
    AAATATGACT ACTTCAGGAA GAAATTAAAG AAACCTGCTG ATATCCCCAA TAGGTTTGAA
    ATGAAACTTC ACAGAAATAA CATATTTGAA GAGTCCTATC GGAGAATTAT GTCCGTGAAA
    AGACCAGATG TCCTAAAAGC TAGACTGTGG ATTGAGTTTG AATCAGAGAA AGGTCTTGAC
    TATGGGGGTG TGGCCAGAGA ATGGTTCTTC TTACTGTCCA AAGAGATGTT CAACCCCTAC
    TACGGCCTCT TTGAGTACTC TGCCACGGAC AACTACACCC TTCAGATCAA CCCTAATTCA
    GGCCTCTGTA ATGAGGATCA TTTGTCCTAC TTCACTTTTA TTGGAAGAGT TGCTGGTCTG
    GCCGTATTTC ATGGGAAGCT CTTAGATGGT TTCTTCATTA GACCATTTTA CAAGATGATG
    TTGGGAAAGC AGATAACCCT GAATGACATG GAATCTGTGG ATAGTGAATA TTACAACTCT
    TTGAAATGGA TCCTGGAGAA TGACCCTACT GAGCTGGACC TCATGTTCTG CATAGACGAA
    GAAAACTTTG GACAGACATA TCAAGTGGAT TTGAAGCCCA ATGGGTCAGA AATAATGGTC
    ACAAATGAAA ACAAAAGGGA ATATATCGAC TTAGTCATCC AGTGGAGATT TGTGAACAGG
    GTCCAGAAGC AGATGAACGC CTTCTTGGAG GGATTCACAG AACTACTTCC TATTGATTTG
    ATTAAAATTT TTGATGAAAA TGAGCTGGAG TTGCTCATGT GCGGCCTCGG TGATGTGGAT
    GTGAATGACT GGAGACAGCA TTCTATTTAC AAGAACGGCT ACTGCCCAAA CCACCCCGTC
    ATTCAGTGGT TCTGGAAGGC TGTGCTACTC ATGGACGCCG AAAAGCGTAT CCGGTTACTG
    CAGTTTGTCA CAGGGACATC GCGAGTACCT ATGAATGGAT TTGCCGAACT TTATGGTTCC
    AATGGTCCTC AGCTGTTTAC AATAGAGCAA TGGGGCAGTC CTGAGAAACT GCCCAGAGCT
    CACACATGCT TTAATCGCCT TGACTTACCT CCATATGAAA CCTTTGAAGA TTTACGAGAG
    AAACTTCTCA TGGCCGTGGA AAATGCTCAA GGATTTGAAG GGGTGGATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu87832
    Clone ID Related Accession (Same CDS sequence) XM_017025677.1
    Accession Version XM_017025677.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2517bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGCTCCTG AAATCCGCAG GACGAACTCC GCGGAGAGGA CTCCGCAGGG CCAGGGGTGC 
    ACATTTAAGA TCAGGCAGGA TCAGAACGCG GGGCAGCAGC ATTTTAGAAT TCTTGTAACC
    CGGGGGCCGG AAGAAGCTCC CCAGAATCCC GAGGAGAAAA GCGCCAAAAG CCCTGTTTCC
    ACGGGAGCTG ACACCACGAC ACGAGACGAC TTCCTGGGCC AGGTGGACGT GCCCCTTAGT
    CACCTTCCGA CAGAAGATCC AACCATGGAG CGACCCTATA CATTTAAGGA CTTTCTCCTC
    AGACCAAGAA GTCATAAGTC TCGAGTTAAG GGATTTTTGC GATTGAAAAT GGCCTATATG
    CCAAAAAATG GAGGTCAAGA TGAAGAAAAC AGTGACCAGA GGGATGACAT GGAGCATGGA
    TGGGAAGTTG TTGACTCAAA TGACTCGGCT TCTCAGCACC AAGAGGAACT TCCTCCTCCT
    CCTCTGCCTC CCGGGTGGGA AGAAAAAGTG GACAATTTAG GCCGAACTTA CTATGTCAAC
    CACAACAACC GGACCACTCA GTGGCACAGA CCAAGCCTGA TGGACGTGTC CTCGGAGTCG
    GACAATAACA TCAGACAGAT CAACCAGGAG GCAGCACACC GGCGCTTCCG CTCCCGCAGG
    CACATCAGCG AAGACTTGGA GCCCGAGCCC TCGGAGGGCG GGGATGTCCC CGAGCCTTGG
    GAGACCATTT CAGAGGAAGT GAATATCGCT GGAGACTCTC TCGGTCTGGC TCTGCCCCCA
    CCACCGGCCT CCCCAGGATC TCGGACCAGC CCTCAGGAGC TGTCAGAGGA ACTAAGCAGA
    AGGCTTCAGA TCACTCCAGA CTCCAATGGG GAACAGTTCA GCTCTTTGAT TCAAAGAGAA
    CCCTCCTCAA GGTTGAGGTC ATGCAGTGTC ACCGACGCAG TTGCAGAACA GGGCCATCTA
    CCACCGGGTG CCAAGGACTC ACCCGTACGT CGGGCTGTGA AAGACACCCT TTCCAACCCA
    CAGTCCCCAC AGCCATCACC TTACAACTCC CCCAAACCAC AACACAAAGT CACACAGAGC
    TTCTTGCCAC CCGGCTGGGA AATGAGGATA GCGCCAAACG GCCGGCCCTT CTTCATTGAT
    CATAACACAA AGACTACAAC CTGGGAAGAT CCACGTTTGA AATTTCCAGT ACATATGCGG
    TCAAAGACAT CTTTAAACCC CAATGACCTT GGCCCCCTTC CTCCTGGCTG GGAAGAAAGA
    ATTCACTTGG ATGGCCGAAC GTTTTATATT GATCATAATA GCAAAATTAC TCAGTGGGAA
    GACCCAAGAC TGCAGAACCC AGCTATTACT GGTCCGGCTG TCCCTTACTC CAGAGAATTT
    AAGCAGAAAT ATGACTACTT CAGGAAGAAA TTAAAGAAAC CTGCTGATAT CCCCAATAGG
    TTTGAAATGA AACTTCACAG AAATAACATA TTTGAAGAGT CCTATCGGAG AATTATGTCC
    GTGAAAAGAC CAGATGTCCT AAAAGCTAGA CTGTGGATTG AGTTTGAATC AGAGAAAGGT
    CTTGACTATG GGGGTGTGGC CAGAGAATGG TTCTTCTTAC TGTCCAAAGA GATGTTCAAC
    CCCTACTACG GCCTCTTTGA GTACTCTGCC ACGGACAACT ACACCCTTCA GATCAACCCT
    AATTCAGGCC TCTGTAATGA GGATCATTTG TCCTACTTCA CTTTTATTGG AAGAGTTGCT
    GGTCTGGCCG TATTTCATGG GAAGCTCTTA GATGGTTTCT TCATTAGACC ATTTTACAAG
    ATGATGTTGG GAAAGCAGAT AACCCTGAAT GACATGGAAT CTGTGGATAG TGAATATTAC
    AACTCTTTGA AATGGATCCT GGAGAATGAC CCTACTGAGC TGGACCTCAT GTTCTGCATA
    GACGAAGAAA ACTTTGGACA GACATATCAA GTGGATTTGA AGCCCAATGG GTCAGAAATA
    ATGGTCACAA ATGAAAACAA AAGGGAATAT ATCGACTTAG TCATCCAGTG GAGATTTGTG
    AACAGGGTCC AGAAGCAGAT GAACGCCTTC TTGGAGGGAT TCACAGAACT ACTTCCTATT
    GATTTGATTA AAATTTTTGA TGAAAATGAG CTGGAGTTGC TCATGTGCGG CCTCGGTGAT
    GTGGATGTGA ATGACTGGAG ACAGCATTCT ATTTACAAGA ACGGCTACTG CCCAAACCAC
    CCCGTCATTC AGTGGTTCTG GAAGGCTGTG CTACTCATGG ACGCCGAAAA GCGTATCCGG
    TTACTGCAGT TTGTCACAGG GACATCGCGA GTACCTATGA ATGGATTTGC CGAACTTTAT
    GGTTCCAATG GTCCTCAGCT GTTTACAATA GAGCAATGGG GCAGTCCTGA GAAACTGCCC
    AGAGCTCACA CATGCTTTAA TCGCCTTGAC TTACCTCCAT ATGAAACCTT TGAAGATTTA
    CGAGAGAAAC TTCTCATGGC CGTGGAAAAT GCTCAAGGAT TTGAAGGGGT GGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016881166.1
    CDS259..2775
    Translation

    Target ORF information:

    RefSeq Version XM_017025677.1
    Organism Homo sapiens(human)
    Definition Homo sapiens NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017025677.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGCTCCTG AAATCCGCAG GACGAACTCC GCGGAGAGGA CTCCGCAGGG CCAGGGGTGC 
    ACATTTAAGA TCAGGCAGGA TCAGAACGCG GGGCAGCAGC ATTTTAGAAT TCTTGTAACC
    CGGGGGCCGG AAGAAGCTCC CCAGAATCCC GAGGAGAAAA GCGCCAAAAG CCCTGTTTCC
    ACGGGAGCTG ACACCACGAC ACGAGACGAC TTCCTGGGCC AGGTGGACGT GCCCCTTAGT
    CACCTTCCGA CAGAAGATCC AACCATGGAG CGACCCTATA CATTTAAGGA CTTTCTCCTC
    AGACCAAGAA GTCATAAGTC TCGAGTTAAG GGATTTTTGC GATTGAAAAT GGCCTATATG
    CCAAAAAATG GAGGTCAAGA TGAAGAAAAC AGTGACCAGA GGGATGACAT GGAGCATGGA
    TGGGAAGTTG TTGACTCAAA TGACTCGGCT TCTCAGCACC AAGAGGAACT TCCTCCTCCT
    CCTCTGCCTC CCGGGTGGGA AGAAAAAGTG GACAATTTAG GCCGAACTTA CTATGTCAAC
    CACAACAACC GGACCACTCA GTGGCACAGA CCAAGCCTGA TGGACGTGTC CTCGGAGTCG
    GACAATAACA TCAGACAGAT CAACCAGGAG GCAGCACACC GGCGCTTCCG CTCCCGCAGG
    CACATCAGCG AAGACTTGGA GCCCGAGCCC TCGGAGGGCG GGGATGTCCC CGAGCCTTGG
    GAGACCATTT CAGAGGAAGT GAATATCGCT GGAGACTCTC TCGGTCTGGC TCTGCCCCCA
    CCACCGGCCT CCCCAGGATC TCGGACCAGC CCTCAGGAGC TGTCAGAGGA ACTAAGCAGA
    AGGCTTCAGA TCACTCCAGA CTCCAATGGG GAACAGTTCA GCTCTTTGAT TCAAAGAGAA
    CCCTCCTCAA GGTTGAGGTC ATGCAGTGTC ACCGACGCAG TTGCAGAACA GGGCCATCTA
    CCACCGGGTG CCAAGGACTC ACCCGTACGT CGGGCTGTGA AAGACACCCT TTCCAACCCA
    CAGTCCCCAC AGCCATCACC TTACAACTCC CCCAAACCAC AACACAAAGT CACACAGAGC
    TTCTTGCCAC CCGGCTGGGA AATGAGGATA GCGCCAAACG GCCGGCCCTT CTTCATTGAT
    CATAACACAA AGACTACAAC CTGGGAAGAT CCACGTTTGA AATTTCCAGT ACATATGCGG
    TCAAAGACAT CTTTAAACCC CAATGACCTT GGCCCCCTTC CTCCTGGCTG GGAAGAAAGA
    ATTCACTTGG ATGGCCGAAC GTTTTATATT GATCATAATA GCAAAATTAC TCAGTGGGAA
    GACCCAAGAC TGCAGAACCC AGCTATTACT GGTCCGGCTG TCCCTTACTC CAGAGAATTT
    AAGCAGAAAT ATGACTACTT CAGGAAGAAA TTAAAGAAAC CTGCTGATAT CCCCAATAGG
    TTTGAAATGA AACTTCACAG AAATAACATA TTTGAAGAGT CCTATCGGAG AATTATGTCC
    GTGAAAAGAC CAGATGTCCT AAAAGCTAGA CTGTGGATTG AGTTTGAATC AGAGAAAGGT
    CTTGACTATG GGGGTGTGGC CAGAGAATGG TTCTTCTTAC TGTCCAAAGA GATGTTCAAC
    CCCTACTACG GCCTCTTTGA GTACTCTGCC ACGGACAACT ACACCCTTCA GATCAACCCT
    AATTCAGGCC TCTGTAATGA GGATCATTTG TCCTACTTCA CTTTTATTGG AAGAGTTGCT
    GGTCTGGCCG TATTTCATGG GAAGCTCTTA GATGGTTTCT TCATTAGACC ATTTTACAAG
    ATGATGTTGG GAAAGCAGAT AACCCTGAAT GACATGGAAT CTGTGGATAG TGAATATTAC
    AACTCTTTGA AATGGATCCT GGAGAATGAC CCTACTGAGC TGGACCTCAT GTTCTGCATA
    GACGAAGAAA ACTTTGGACA GACATATCAA GTGGATTTGA AGCCCAATGG GTCAGAAATA
    ATGGTCACAA ATGAAAACAA AAGGGAATAT ATCGACTTAG TCATCCAGTG GAGATTTGTG
    AACAGGGTCC AGAAGCAGAT GAACGCCTTC TTGGAGGGAT TCACAGAACT ACTTCCTATT
    GATTTGATTA AAATTTTTGA TGAAAATGAG CTGGAGTTGC TCATGTGCGG CCTCGGTGAT
    GTGGATGTGA ATGACTGGAG ACAGCATTCT ATTTACAAGA ACGGCTACTG CCCAAACCAC
    CCCGTCATTC AGTGGTTCTG GAAGGCTGTG CTACTCATGG ACGCCGAAAA GCGTATCCGG
    TTACTGCAGT TTGTCACAGG GACATCGCGA GTACCTATGA ATGGATTTGC CGAACTTTAT
    GGTTCCAATG GTCCTCAGCT GTTTACAATA GAGCAATGGG GCAGTCCTGA GAAACTGCCC
    AGAGCTCACA CATGCTTTAA TCGCCTTGAC TTACCTCCAT ATGAAACCTT TGAAGATTTA
    CGAGAGAAAC TTCTCATGGC CGTGGAAAAT GCTCAAGGAT TTGAAGGGGT GGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu87833
    Clone ID Related Accession (Same CDS sequence) XM_017025678.1 , XM_017025678.2
    Accession Version XM_017025678.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2922bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGCGACCG GGCTCGGGGA GCCGGTCTAT GGACTTTCCG AAGACGAGGG AGAGTCCCGT 
    ATTCTCAGAG TAAAAGTTGT TTCTGGAATT GATCTCGCCA AAAAGGACAT CTTTGGAGCC
    AGTGATCCGT ATGTGAAACT TTCATTGTAC GTAGCGGATG AGAATAGAGA ACTTGCTTTG
    GTCCAGACAA AAACAATTAA AAAGACACTG AACCCAAAAT GGAATGAAGA ATTTTATTTC
    AGGGTAAACC CATCTAATCA CAGACTCCTA TTTGAAGTAT TTGACGAAAA TAGACTGACA
    CGAGACGACT TCCTGGGCCA GGTGGACGTG CCCCTTAGTC ACCTTCCGAC AGAAGATCCA
    ACCATGGAGC GACCCTATAC ATTTAAGGAC TTTCTCCTCA GACCAAGAAG TCATAAGTCT
    CGAGTTAAGG GATTTTTGCG ATTGAAAATG GCCTATATGC CAAAAAATGG AGGTCAAGAT
    GAAGAAAACA GTGACCAGAG GGATGACATG GAGCATGGAT GGGAAGTTGT TGACTCAAAT
    GACTCGGCTT CTCAGCACCA AGAGGAACTT CCTCCTCCTC CTCTGCCTCC CGGGTGGGAA
    GAAAAAGTGG ACAATTTAGG CCGAACTTAC TATGTCAACC ACAACAACCG GACCACTCAG
    TGGCACAGAC CAAGCCTGAT GGACGTGTCC TCGGAGTCGG ACAATAACAT CAGACAGATC
    AACCAGGAGG CAGCACACCG GCGCTTCCGC TCCCGCAGGC ACATCAGCGA AGACTTGGAG
    CCCGAGCCCT CGGAGGGCGG GGATGTCCCC GAGCCTTGGG AGACCATTTC AGAGGAAGTG
    AATATCGCTG GAGACTCTCT CGGTCTGGCT CTGCCCCCAC CACCGGCCTC CCCAGGATCT
    CGGACCAGCC CTCAGGAGCT GTCAGAGGAA CTAAGCAGAA GGCTTCAGAT CACTCCAGAC
    TCCAATGGGG AACAGTTCAG CTCTTTGATT CAAAGAGAAC CCTCCTCAAG GTTGAGGTCA
    TGCAGTGTCA CCGACGCAGT TGCAGAACAG GGCCATCTAC CACCGCCATC AGTGGCCTAT
    GTACATACCA CGCCGGGTCT GCCTTCAGGC TGGGAAGAAA GAAAAGATGC TAAGGGGCGC
    ACATACTATG TCAATCATAA CAATCGAACC ACAACTTGGA CTCGACCTAT CATGCAGCTT
    GCAGAAGATG GTGCGTCCGG ATCAGCCACA AACAGTAACA ACCATCTAAT CGAGCCTCAG
    ATCCGCCGGC CTCGTAGCCT CAGCTCGCCA ACAGTAACTT TATCTGCCCC GCTGGAGGGT
    GCCAAGGACT CACCCGTACG TCGGGCTGTG AAAGACACCC TTTCCAACCC ACAGTCCCCA
    CAGCCATCAC CTTACAACTC CCCCAAACCA CAACACAAAG TCACACAGAG CTTCTTGCCA
    CCCGGCTGGG AAATGAGGAT AGCGCCAAAC GGCCGGCCCT TCTTCATTGA TCATAACACA
    AAGACTACAA CCTGGGAAGA TCCACGTTTG AAATTTCCAG TACATATGCG GTCAAAGACA
    TCTTTAAACC CCAATGACCT TGGCCCCCTT CCTCCTGGCT GGGAAGAAAG AATTCACTTG
    GATGGCCGAA CGTTTTATAT TGATCATAGC AGCCAGGTGT TATGTGGAGA AAATGATACC
    AGAGAATCAG TGCCTTCATA CGATAGCAAA ATTACTCAGT GGGAAGACCC AAGACTGCAG
    AACCCAGCTA TTACTGGTCC GGCTGTCCCT TACTCCAGAG AATTTAAGCA GAAATATGAC
    TACTTCAGGA AGAAATTAAA GAAACCTGCT GATATCCCCA ATAGGTTTGA AATGAAACTT
    CACAGAAATA ACATATTTGA AGAGTCCTAT CGGAGAATTA TGTCCGTGAA AAGACCAGAT
    GTCCTAAAAG CTAGACTGTG GATTGAGTTT GAATCAGAGA AAGGTCTTGA CTATGGGGGT
    GTGGCCAGAG AATGGTTCTT CTTACTGTCC AAAGAGATGT TCAACCCCTA CTACGGCCTC
    TTTGAGTACT CTGCCACGGA CAACTACACC CTTCAGATCA ACCCTAATTC AGGCCTCTGT
    AATGAGGATC ATTTGTCCTA CTTCACTTTT ATTGGAAGAG TTGCTGGTCT GGCCGTATTT
    CATGGGAAGC TCTTAGATGG TTTCTTCATT AGACCATTTT ACAAGATGAT GTTGGGAAAG
    CAGATAACCC TGAATGACAT GGAATCTGTG GATAGTGAAT ATTACAACTC TTTGAAATGG
    ATCCTGGAGA ATGACCCTAC TGAGCTGGAC CTCATGTTCT GCATAGACGA AGAAAACTTT
    GGACAGACAT ATCAAGTGGA TTTGAAGCCC AATGGGTCAG AAATAATGGT CACAAATGAA
    AACAAAAGGG AATATATCGA CTTAGTCATC CAGTGGAGAT TTGTGAACAG GGTCCAGAAG
    CAGATGAACG CCTTCTTGGA GGGATTCACA GAACTACTTC CTATTGATTT GATTAAAATT
    TTTGATGAAA ATGAGCTGGA GTTGCTCATG TGCGGCCTCG GTGATGTGGA TGTGAATGAC
    TGGAGACAGC ATTCTATTTA CAAGAACGGC TACTGCCCAA ACCACCCCGT CATTCAGTGG
    TTCTGGAAGG CTGTGCTACT CATGGACGCC GAAAAGCGTA TCCGGTTACT GCAGTTTGTC
    ACAGGGACAT CGCGAGTACC TATGAATGGA TTTGCCGAAC TTTATGGTTC CAATGGTCCT
    CAGCTGTTTA CAATAGAGCA ATGGGGCAGT CCTGAGAAAC TGCCCAGAGC TCACACATGC
    TTTAATCGCC TTGACTTACC TCCATATGAA ACCTTTGAAG ATTTACGAGA GAAACTTCTC
    ATGGCCGTGG AAAATGCTCA AGGATTTGAA GGGGTGGATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016881167.1
    CDS299..3220
    Translation

    Target ORF information:

    RefSeq Version XM_017025678.1
    Organism Homo sapiens(human)
    Definition Homo sapiens neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017025678.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGCGACCG GGCTCGGGGA GCCGGTCTAT GGACTTTCCG AAGACGAGGG AGAGTCCCGT 
    ATTCTCAGAG TAAAAGTTGT TTCTGGAATT GATCTCGCCA AAAAGGACAT CTTTGGAGCC
    AGTGATCCGT ATGTGAAACT TTCATTGTAC GTAGCGGATG AGAATAGAGA ACTTGCTTTG
    GTCCAGACAA AAACAATTAA AAAGACACTG AACCCAAAAT GGAATGAAGA ATTTTATTTC
    AGGGTAAACC CATCTAATCA CAGACTCCTA TTTGAAGTAT TTGACGAAAA TAGACTGACA
    CGAGACGACT TCCTGGGCCA GGTGGACGTG CCCCTTAGTC ACCTTCCGAC AGAAGATCCA
    ACCATGGAGC GACCCTATAC ATTTAAGGAC TTTCTCCTCA GACCAAGAAG TCATAAGTCT
    CGAGTTAAGG GATTTTTGCG ATTGAAAATG GCCTATATGC CAAAAAATGG AGGTCAAGAT
    GAAGAAAACA GTGACCAGAG GGATGACATG GAGCATGGAT GGGAAGTTGT TGACTCAAAT
    GACTCGGCTT CTCAGCACCA AGAGGAACTT CCTCCTCCTC CTCTGCCTCC CGGGTGGGAA
    GAAAAAGTGG ACAATTTAGG CCGAACTTAC TATGTCAACC ACAACAACCG GACCACTCAG
    TGGCACAGAC CAAGCCTGAT GGACGTGTCC TCGGAGTCGG ACAATAACAT CAGACAGATC
    AACCAGGAGG CAGCACACCG GCGCTTCCGC TCCCGCAGGC ACATCAGCGA AGACTTGGAG
    CCCGAGCCCT CGGAGGGCGG GGATGTCCCC GAGCCTTGGG AGACCATTTC AGAGGAAGTG
    AATATCGCTG GAGACTCTCT CGGTCTGGCT CTGCCCCCAC CACCGGCCTC CCCAGGATCT
    CGGACCAGCC CTCAGGAGCT GTCAGAGGAA CTAAGCAGAA GGCTTCAGAT CACTCCAGAC
    TCCAATGGGG AACAGTTCAG CTCTTTGATT CAAAGAGAAC CCTCCTCAAG GTTGAGGTCA
    TGCAGTGTCA CCGACGCAGT TGCAGAACAG GGCCATCTAC CACCGCCATC AGTGGCCTAT
    GTACATACCA CGCCGGGTCT GCCTTCAGGC TGGGAAGAAA GAAAAGATGC TAAGGGGCGC
    ACATACTATG TCAATCATAA CAATCGAACC ACAACTTGGA CTCGACCTAT CATGCAGCTT
    GCAGAAGATG GTGCGTCCGG ATCAGCCACA AACAGTAACA ACCATCTAAT CGAGCCTCAG
    ATCCGCCGGC CTCGTAGCCT CAGCTCGCCA ACAGTAACTT TATCTGCCCC GCTGGAGGGT
    GCCAAGGACT CACCCGTACG TCGGGCTGTG AAAGACACCC TTTCCAACCC ACAGTCCCCA
    CAGCCATCAC CTTACAACTC CCCCAAACCA CAACACAAAG TCACACAGAG CTTCTTGCCA
    CCCGGCTGGG AAATGAGGAT AGCGCCAAAC GGCCGGCCCT TCTTCATTGA TCATAACACA
    AAGACTACAA CCTGGGAAGA TCCACGTTTG AAATTTCCAG TACATATGCG GTCAAAGACA
    TCTTTAAACC CCAATGACCT TGGCCCCCTT CCTCCTGGCT GGGAAGAAAG AATTCACTTG
    GATGGCCGAA CGTTTTATAT TGATCATAGC AGCCAGGTGT TATGTGGAGA AAATGATACC
    AGAGAATCAG TGCCTTCATA CGATAGCAAA ATTACTCAGT GGGAAGACCC AAGACTGCAG
    AACCCAGCTA TTACTGGTCC GGCTGTCCCT TACTCCAGAG AATTTAAGCA GAAATATGAC
    TACTTCAGGA AGAAATTAAA GAAACCTGCT GATATCCCCA ATAGGTTTGA AATGAAACTT
    CACAGAAATA ACATATTTGA AGAGTCCTAT CGGAGAATTA TGTCCGTGAA AAGACCAGAT
    GTCCTAAAAG CTAGACTGTG GATTGAGTTT GAATCAGAGA AAGGTCTTGA CTATGGGGGT
    GTGGCCAGAG AATGGTTCTT CTTACTGTCC AAAGAGATGT TCAACCCCTA CTACGGCCTC
    TTTGAGTACT CTGCCACGGA CAACTACACC CTTCAGATCA ACCCTAATTC AGGCCTCTGT
    AATGAGGATC ATTTGTCCTA CTTCACTTTT ATTGGAAGAG TTGCTGGTCT GGCCGTATTT
    CATGGGAAGC TCTTAGATGG TTTCTTCATT AGACCATTTT ACAAGATGAT GTTGGGAAAG
    CAGATAACCC TGAATGACAT GGAATCTGTG GATAGTGAAT ATTACAACTC TTTGAAATGG
    ATCCTGGAGA ATGACCCTAC TGAGCTGGAC CTCATGTTCT GCATAGACGA AGAAAACTTT
    GGACAGACAT ATCAAGTGGA TTTGAAGCCC AATGGGTCAG AAATAATGGT CACAAATGAA
    AACAAAAGGG AATATATCGA CTTAGTCATC CAGTGGAGAT TTGTGAACAG GGTCCAGAAG
    CAGATGAACG CCTTCTTGGA GGGATTCACA GAACTACTTC CTATTGATTT GATTAAAATT
    TTTGATGAAA ATGAGCTGGA GTTGCTCATG TGCGGCCTCG GTGATGTGGA TGTGAATGAC
    TGGAGACAGC ATTCTATTTA CAAGAACGGC TACTGCCCAA ACCACCCCGT CATTCAGTGG
    TTCTGGAAGG CTGTGCTACT CATGGACGCC GAAAAGCGTA TCCGGTTACT GCAGTTTGTC
    ACAGGGACAT CGCGAGTACC TATGAATGGA TTTGCCGAAC TTTATGGTTC CAATGGTCCT
    CAGCTGTTTA CAATAGAGCA ATGGGGCAGT CCTGAGAAAC TGCCCAGAGC TCACACATGC
    TTTAATCGCC TTGACTTACC TCCATATGAA ACCTTTGAAG ATTTACGAGA GAAACTTCTC
    ATGGCCGTGG AAAATGCTCA AGGATTTGAA GGGGTGGATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu87833
    Clone ID Related Accession (Same CDS sequence) XM_017025678.1 , XM_017025678.2
    Accession Version XM_017025678.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2922bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017025678.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGCGACCG GGCTCGGGGA GCCGGTCTAT GGACTTTCCG AAGACGAGGG AGAGTCCCGT 
    ATTCTCAGAG TAAAAGTTGT TTCTGGAATT GATCTCGCCA AAAAGGACAT CTTTGGAGCC
    AGTGATCCGT ATGTGAAACT TTCATTGTAC GTAGCGGATG AGAATAGAGA ACTTGCTTTG
    GTCCAGACAA AAACAATTAA AAAGACACTG AACCCAAAAT GGAATGAAGA ATTTTATTTC
    AGGGTAAACC CATCTAATCA CAGACTCCTA TTTGAAGTAT TTGACGAAAA TAGACTGACA
    CGAGACGACT TCCTGGGCCA GGTGGACGTG CCCCTTAGTC ACCTTCCGAC AGAAGATCCA
    ACCATGGAGC GACCCTATAC ATTTAAGGAC TTTCTCCTCA GACCAAGAAG TCATAAGTCT
    CGAGTTAAGG GATTTTTGCG ATTGAAAATG GCCTATATGC CAAAAAATGG AGGTCAAGAT
    GAAGAAAACA GTGACCAGAG GGATGACATG GAGCATGGAT GGGAAGTTGT TGACTCAAAT
    GACTCGGCTT CTCAGCACCA AGAGGAACTT CCTCCTCCTC CTCTGCCTCC CGGGTGGGAA
    GAAAAAGTGG ACAATTTAGG CCGAACTTAC TATGTCAACC ACAACAACCG GACCACTCAG
    TGGCACAGAC CAAGCCTGAT GGACGTGTCC TCGGAGTCGG ACAATAACAT CAGACAGATC
    AACCAGGAGG CAGCACACCG GCGCTTCCGC TCCCGCAGGC ACATCAGCGA AGACTTGGAG
    CCCGAGCCCT CGGAGGGCGG GGATGTCCCC GAGCCTTGGG AGACCATTTC AGAGGAAGTG
    AATATCGCTG GAGACTCTCT CGGTCTGGCT CTGCCCCCAC CACCGGCCTC CCCAGGATCT
    CGGACCAGCC CTCAGGAGCT GTCAGAGGAA CTAAGCAGAA GGCTTCAGAT CACTCCAGAC
    TCCAATGGGG AACAGTTCAG CTCTTTGATT CAAAGAGAAC CCTCCTCAAG GTTGAGGTCA
    TGCAGTGTCA CCGACGCAGT TGCAGAACAG GGCCATCTAC CACCGCCATC AGTGGCCTAT
    GTACATACCA CGCCGGGTCT GCCTTCAGGC TGGGAAGAAA GAAAAGATGC TAAGGGGCGC
    ACATACTATG TCAATCATAA CAATCGAACC ACAACTTGGA CTCGACCTAT CATGCAGCTT
    GCAGAAGATG GTGCGTCCGG ATCAGCCACA AACAGTAACA ACCATCTAAT CGAGCCTCAG
    ATCCGCCGGC CTCGTAGCCT CAGCTCGCCA ACAGTAACTT TATCTGCCCC GCTGGAGGGT
    GCCAAGGACT CACCCGTACG TCGGGCTGTG AAAGACACCC TTTCCAACCC ACAGTCCCCA
    CAGCCATCAC CTTACAACTC CCCCAAACCA CAACACAAAG TCACACAGAG CTTCTTGCCA
    CCCGGCTGGG AAATGAGGAT AGCGCCAAAC GGCCGGCCCT TCTTCATTGA TCATAACACA
    AAGACTACAA CCTGGGAAGA TCCACGTTTG AAATTTCCAG TACATATGCG GTCAAAGACA
    TCTTTAAACC CCAATGACCT TGGCCCCCTT CCTCCTGGCT GGGAAGAAAG AATTCACTTG
    GATGGCCGAA CGTTTTATAT TGATCATAGC AGCCAGGTGT TATGTGGAGA AAATGATACC
    AGAGAATCAG TGCCTTCATA CGATAGCAAA ATTACTCAGT GGGAAGACCC AAGACTGCAG
    AACCCAGCTA TTACTGGTCC GGCTGTCCCT TACTCCAGAG AATTTAAGCA GAAATATGAC
    TACTTCAGGA AGAAATTAAA GAAACCTGCT GATATCCCCA ATAGGTTTGA AATGAAACTT
    CACAGAAATA ACATATTTGA AGAGTCCTAT CGGAGAATTA TGTCCGTGAA AAGACCAGAT
    GTCCTAAAAG CTAGACTGTG GATTGAGTTT GAATCAGAGA AAGGTCTTGA CTATGGGGGT
    GTGGCCAGAG AATGGTTCTT CTTACTGTCC AAAGAGATGT TCAACCCCTA CTACGGCCTC
    TTTGAGTACT CTGCCACGGA CAACTACACC CTTCAGATCA ACCCTAATTC AGGCCTCTGT
    AATGAGGATC ATTTGTCCTA CTTCACTTTT ATTGGAAGAG TTGCTGGTCT GGCCGTATTT
    CATGGGAAGC TCTTAGATGG TTTCTTCATT AGACCATTTT ACAAGATGAT GTTGGGAAAG
    CAGATAACCC TGAATGACAT GGAATCTGTG GATAGTGAAT ATTACAACTC TTTGAAATGG
    ATCCTGGAGA ATGACCCTAC TGAGCTGGAC CTCATGTTCT GCATAGACGA AGAAAACTTT
    GGACAGACAT ATCAAGTGGA TTTGAAGCCC AATGGGTCAG AAATAATGGT CACAAATGAA
    AACAAAAGGG AATATATCGA CTTAGTCATC CAGTGGAGAT TTGTGAACAG GGTCCAGAAG
    CAGATGAACG CCTTCTTGGA GGGATTCACA GAACTACTTC CTATTGATTT GATTAAAATT
    TTTGATGAAA ATGAGCTGGA GTTGCTCATG TGCGGCCTCG GTGATGTGGA TGTGAATGAC
    TGGAGACAGC ATTCTATTTA CAAGAACGGC TACTGCCCAA ACCACCCCGT CATTCAGTGG
    TTCTGGAAGG CTGTGCTACT CATGGACGCC GAAAAGCGTA TCCGGTTACT GCAGTTTGTC
    ACAGGGACAT CGCGAGTACC TATGAATGGA TTTGCCGAAC TTTATGGTTC CAATGGTCCT
    CAGCTGTTTA CAATAGAGCA ATGGGGCAGT CCTGAGAAAC TGCCCAGAGC TCACACATGC
    TTTAATCGCC TTGACTTACC TCCATATGAA ACCTTTGAAG ATTTACGAGA GAAACTTCTC
    ATGGCCGTGG AAAATGCTCA AGGATTTGAA GGGGTGGATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016881167.1
    CDS428..3349
    Translation

    Target ORF information:

    RefSeq Version XM_017025678.2
    Organism Homo sapiens(human)
    Definition Homo sapiens NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017025678.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGCGACCG GGCTCGGGGA GCCGGTCTAT GGACTTTCCG AAGACGAGGG AGAGTCCCGT 
    ATTCTCAGAG TAAAAGTTGT TTCTGGAATT GATCTCGCCA AAAAGGACAT CTTTGGAGCC
    AGTGATCCGT ATGTGAAACT TTCATTGTAC GTAGCGGATG AGAATAGAGA ACTTGCTTTG
    GTCCAGACAA AAACAATTAA AAAGACACTG AACCCAAAAT GGAATGAAGA ATTTTATTTC
    AGGGTAAACC CATCTAATCA CAGACTCCTA TTTGAAGTAT TTGACGAAAA TAGACTGACA
    CGAGACGACT TCCTGGGCCA GGTGGACGTG CCCCTTAGTC ACCTTCCGAC AGAAGATCCA
    ACCATGGAGC GACCCTATAC ATTTAAGGAC TTTCTCCTCA GACCAAGAAG TCATAAGTCT
    CGAGTTAAGG GATTTTTGCG ATTGAAAATG GCCTATATGC CAAAAAATGG AGGTCAAGAT
    GAAGAAAACA GTGACCAGAG GGATGACATG GAGCATGGAT GGGAAGTTGT TGACTCAAAT
    GACTCGGCTT CTCAGCACCA AGAGGAACTT CCTCCTCCTC CTCTGCCTCC CGGGTGGGAA
    GAAAAAGTGG ACAATTTAGG CCGAACTTAC TATGTCAACC ACAACAACCG GACCACTCAG
    TGGCACAGAC CAAGCCTGAT GGACGTGTCC TCGGAGTCGG ACAATAACAT CAGACAGATC
    AACCAGGAGG CAGCACACCG GCGCTTCCGC TCCCGCAGGC ACATCAGCGA AGACTTGGAG
    CCCGAGCCCT CGGAGGGCGG GGATGTCCCC GAGCCTTGGG AGACCATTTC AGAGGAAGTG
    AATATCGCTG GAGACTCTCT CGGTCTGGCT CTGCCCCCAC CACCGGCCTC CCCAGGATCT
    CGGACCAGCC CTCAGGAGCT GTCAGAGGAA CTAAGCAGAA GGCTTCAGAT CACTCCAGAC
    TCCAATGGGG AACAGTTCAG CTCTTTGATT CAAAGAGAAC CCTCCTCAAG GTTGAGGTCA
    TGCAGTGTCA CCGACGCAGT TGCAGAACAG GGCCATCTAC CACCGCCATC AGTGGCCTAT
    GTACATACCA CGCCGGGTCT GCCTTCAGGC TGGGAAGAAA GAAAAGATGC TAAGGGGCGC
    ACATACTATG TCAATCATAA CAATCGAACC ACAACTTGGA CTCGACCTAT CATGCAGCTT
    GCAGAAGATG GTGCGTCCGG ATCAGCCACA AACAGTAACA ACCATCTAAT CGAGCCTCAG
    ATCCGCCGGC CTCGTAGCCT CAGCTCGCCA ACAGTAACTT TATCTGCCCC GCTGGAGGGT
    GCCAAGGACT CACCCGTACG TCGGGCTGTG AAAGACACCC TTTCCAACCC ACAGTCCCCA
    CAGCCATCAC CTTACAACTC CCCCAAACCA CAACACAAAG TCACACAGAG CTTCTTGCCA
    CCCGGCTGGG AAATGAGGAT AGCGCCAAAC GGCCGGCCCT TCTTCATTGA TCATAACACA
    AAGACTACAA CCTGGGAAGA TCCACGTTTG AAATTTCCAG TACATATGCG GTCAAAGACA
    TCTTTAAACC CCAATGACCT TGGCCCCCTT CCTCCTGGCT GGGAAGAAAG AATTCACTTG
    GATGGCCGAA CGTTTTATAT TGATCATAGC AGCCAGGTGT TATGTGGAGA AAATGATACC
    AGAGAATCAG TGCCTTCATA CGATAGCAAA ATTACTCAGT GGGAAGACCC AAGACTGCAG
    AACCCAGCTA TTACTGGTCC GGCTGTCCCT TACTCCAGAG AATTTAAGCA GAAATATGAC
    TACTTCAGGA AGAAATTAAA GAAACCTGCT GATATCCCCA ATAGGTTTGA AATGAAACTT
    CACAGAAATA ACATATTTGA AGAGTCCTAT CGGAGAATTA TGTCCGTGAA AAGACCAGAT
    GTCCTAAAAG CTAGACTGTG GATTGAGTTT GAATCAGAGA AAGGTCTTGA CTATGGGGGT
    GTGGCCAGAG AATGGTTCTT CTTACTGTCC AAAGAGATGT TCAACCCCTA CTACGGCCTC
    TTTGAGTACT CTGCCACGGA CAACTACACC CTTCAGATCA ACCCTAATTC AGGCCTCTGT
    AATGAGGATC ATTTGTCCTA CTTCACTTTT ATTGGAAGAG TTGCTGGTCT GGCCGTATTT
    CATGGGAAGC TCTTAGATGG TTTCTTCATT AGACCATTTT ACAAGATGAT GTTGGGAAAG
    CAGATAACCC TGAATGACAT GGAATCTGTG GATAGTGAAT ATTACAACTC TTTGAAATGG
    ATCCTGGAGA ATGACCCTAC TGAGCTGGAC CTCATGTTCT GCATAGACGA AGAAAACTTT
    GGACAGACAT ATCAAGTGGA TTTGAAGCCC AATGGGTCAG AAATAATGGT CACAAATGAA
    AACAAAAGGG AATATATCGA CTTAGTCATC CAGTGGAGAT TTGTGAACAG GGTCCAGAAG
    CAGATGAACG CCTTCTTGGA GGGATTCACA GAACTACTTC CTATTGATTT GATTAAAATT
    TTTGATGAAA ATGAGCTGGA GTTGCTCATG TGCGGCCTCG GTGATGTGGA TGTGAATGAC
    TGGAGACAGC ATTCTATTTA CAAGAACGGC TACTGCCCAA ACCACCCCGT CATTCAGTGG
    TTCTGGAAGG CTGTGCTACT CATGGACGCC GAAAAGCGTA TCCGGTTACT GCAGTTTGTC
    ACAGGGACAT CGCGAGTACC TATGAATGGA TTTGCCGAAC TTTATGGTTC CAATGGTCCT
    CAGCTGTTTA CAATAGAGCA ATGGGGCAGT CCTGAGAAAC TGCCCAGAGC TCACACATGC
    TTTAATCGCC TTGACTTACC TCCATATGAA ACCTTTGAAG ATTTACGAGA GAAACTTCTC
    ATGGCCGTGG AAAATGCTCA AGGATTTGAA GGGGTGGATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu87834
    Clone ID Related Accession (Same CDS sequence) XM_017025680.1 , XM_017025680.2 , XM_024451132.1
    Accession Version XM_017025680.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2253bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGAGCGAC CCTATACATT TAAGGACTTT CTCCTCAGAC CAAGAAGTCA TAAGTCTCGA 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCAA AAAATGGAGG TCAAGATGAA
    GAAAACAGTG ACCAGAGGGA TGACATGGAG CATGGATGGG AAGTTGTTGA CTCAAATGAC
    TCGGCTTCTC AGCACCAAGA GGAACTTCCT CCTCCTCCTC TGCCTCCCGG GTGGGAAGAA
    AAAGTGGACA ATTTAGGCCG AACTTACTAT GTCAACCACA ACAACCGGAC CACTCAGTGG
    CACAGACCAA GCCTGATGGA CGTGTCCTCG GAGTCGGACA ATAACATCAG ACAGATCAAC
    CAGGAGGCAG CACACCGGCG CTTCCGCTCC CGCAGGCACA TCAGCGAAGA CTTGGAGCCC
    GAGCCCTCGG AGGGCGGGGA TGTCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAT
    ATCGCTGGAG ACTCTCTCGG TCTGGCTCTG CCCCCACCAC CGGCCTCCCC AGGATCTCGG
    ACCAGCCCTC AGGAGCTGTC AGAGGAACTA AGCAGAAGGC TTCAGATCAC TCCAGACTCC
    AATGGGGAAC AGTTCAGCTC TTTGATTCAA AGAGAACCCT CCTCAAGGTT GAGGTCATGC
    AGTGTCACCG ACGCAGTTGC AGAACAGGGC CATCTACCAC CGGGTGCCAA GGACTCACCC
    GTACGTCGGG CTGTGAAAGA CACCCTTTCC AACCCACAGT CCCCACAGCC ATCACCTTAC
    AACTCCCCCA AACCACAACA CAAAGTCACA CAGAGCTTCT TGCCACCCGG CTGGGAAATG
    AGGATAGCGC CAAACGGCCG GCCCTTCTTC ATTGATCATA ACACAAAGAC TACAACCTGG
    GAAGATCCAC GTTTGAAATT TCCAGTACAT ATGCGGTCAA AGACATCTTT AAACCCCAAT
    GACCTTGGCC CCCTTCCTCC TGGCTGGGAA GAAAGAATTC ACTTGGATGG CCGAACGTTT
    TATATTGATC ATAATAGCAA AATTACTCAG TGGGAAGACC CAAGACTGCA GAACCCAGCT
    ATTACTGGTC CGGCTGTCCC TTACTCCAGA GAATTTAAGC AGAAATATGA CTACTTCAGG
    AAGAAATTAA AGAAACCTGC TGATATCCCC AATAGGTTTG AAATGAAACT TCACAGAAAT
    AACATATTTG AAGAGTCCTA TCGGAGAATT ATGTCCGTGA AAAGACCAGA TGTCCTAAAA
    GCTAGACTGT GGATTGAGTT TGAATCAGAG AAAGGTCTTG ACTATGGGGG TGTGGCCAGA
    GAATGGTTCT TCTTACTGTC CAAAGAGATG TTCAACCCCT ACTACGGCCT CTTTGAGTAC
    TCTGCCACGG ACAACTACAC CCTTCAGATC AACCCTAATT CAGGCCTCTG TAATGAGGAT
    CATTTGTCCT ACTTCACTTT TATTGGAAGA GTTGCTGGTC TGGCCGTATT TCATGGGAAG
    CTCTTAGATG GTTTCTTCAT TAGACCATTT TACAAGATGA TGTTGGGAAA GCAGATAACC
    CTGAATGACA TGGAATCTGT GGATAGTGAA TATTACAACT CTTTGAAATG GATCCTGGAG
    AATGACCCTA CTGAGCTGGA CCTCATGTTC TGCATAGACG AAGAAAACTT TGGACAGACA
    TATCAAGTGG ATTTGAAGCC CAATGGGTCA GAAATAATGG TCACAAATGA AAACAAAAGG
    GAATATATCG ACTTAGTCAT CCAGTGGAGA TTTGTGAACA GGGTCCAGAA GCAGATGAAC
    GCCTTCTTGG AGGGATTCAC AGAACTACTT CCTATTGATT TGATTAAAAT TTTTGATGAA
    AATGAGCTGG AGTTGCTCAT GTGCGGCCTC GGTGATGTGG ATGTGAATGA CTGGAGACAG
    CATTCTATTT ACAAGAACGG CTACTGCCCA AACCACCCCG TCATTCAGTG GTTCTGGAAG
    GCTGTGCTAC TCATGGACGC CGAAAAGCGT ATCCGGTTAC TGCAGTTTGT CACAGGGACA
    TCGCGAGTAC CTATGAATGG ATTTGCCGAA CTTTATGGTT CCAATGGTCC TCAGCTGTTT
    ACAATAGAGC AATGGGGCAG TCCTGAGAAA CTGCCCAGAG CTCACACATG CTTTAATCGC
    CTTGACTTAC CTCCATATGA AACCTTTGAA GATTTACGAG AGAAACTTCT CATGGCCGTG
    GAAAATGCTC AAGGATTTGA AGGGGTGGAT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016881169.1
    CDS571..2823
    Translation

    Target ORF information:

    RefSeq Version XM_017025680.1
    Organism Homo sapiens(human)
    Definition Homo sapiens neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017025680.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGAGCGAC CCTATACATT TAAGGACTTT CTCCTCAGAC CAAGAAGTCA TAAGTCTCGA 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCAA AAAATGGAGG TCAAGATGAA
    GAAAACAGTG ACCAGAGGGA TGACATGGAG CATGGATGGG AAGTTGTTGA CTCAAATGAC
    TCGGCTTCTC AGCACCAAGA GGAACTTCCT CCTCCTCCTC TGCCTCCCGG GTGGGAAGAA
    AAAGTGGACA ATTTAGGCCG AACTTACTAT GTCAACCACA ACAACCGGAC CACTCAGTGG
    CACAGACCAA GCCTGATGGA CGTGTCCTCG GAGTCGGACA ATAACATCAG ACAGATCAAC
    CAGGAGGCAG CACACCGGCG CTTCCGCTCC CGCAGGCACA TCAGCGAAGA CTTGGAGCCC
    GAGCCCTCGG AGGGCGGGGA TGTCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAT
    ATCGCTGGAG ACTCTCTCGG TCTGGCTCTG CCCCCACCAC CGGCCTCCCC AGGATCTCGG
    ACCAGCCCTC AGGAGCTGTC AGAGGAACTA AGCAGAAGGC TTCAGATCAC TCCAGACTCC
    AATGGGGAAC AGTTCAGCTC TTTGATTCAA AGAGAACCCT CCTCAAGGTT GAGGTCATGC
    AGTGTCACCG ACGCAGTTGC AGAACAGGGC CATCTACCAC CGGGTGCCAA GGACTCACCC
    GTACGTCGGG CTGTGAAAGA CACCCTTTCC AACCCACAGT CCCCACAGCC ATCACCTTAC
    AACTCCCCCA AACCACAACA CAAAGTCACA CAGAGCTTCT TGCCACCCGG CTGGGAAATG
    AGGATAGCGC CAAACGGCCG GCCCTTCTTC ATTGATCATA ACACAAAGAC TACAACCTGG
    GAAGATCCAC GTTTGAAATT TCCAGTACAT ATGCGGTCAA AGACATCTTT AAACCCCAAT
    GACCTTGGCC CCCTTCCTCC TGGCTGGGAA GAAAGAATTC ACTTGGATGG CCGAACGTTT
    TATATTGATC ATAATAGCAA AATTACTCAG TGGGAAGACC CAAGACTGCA GAACCCAGCT
    ATTACTGGTC CGGCTGTCCC TTACTCCAGA GAATTTAAGC AGAAATATGA CTACTTCAGG
    AAGAAATTAA AGAAACCTGC TGATATCCCC AATAGGTTTG AAATGAAACT TCACAGAAAT
    AACATATTTG AAGAGTCCTA TCGGAGAATT ATGTCCGTGA AAAGACCAGA TGTCCTAAAA
    GCTAGACTGT GGATTGAGTT TGAATCAGAG AAAGGTCTTG ACTATGGGGG TGTGGCCAGA
    GAATGGTTCT TCTTACTGTC CAAAGAGATG TTCAACCCCT ACTACGGCCT CTTTGAGTAC
    TCTGCCACGG ACAACTACAC CCTTCAGATC AACCCTAATT CAGGCCTCTG TAATGAGGAT
    CATTTGTCCT ACTTCACTTT TATTGGAAGA GTTGCTGGTC TGGCCGTATT TCATGGGAAG
    CTCTTAGATG GTTTCTTCAT TAGACCATTT TACAAGATGA TGTTGGGAAA GCAGATAACC
    CTGAATGACA TGGAATCTGT GGATAGTGAA TATTACAACT CTTTGAAATG GATCCTGGAG
    AATGACCCTA CTGAGCTGGA CCTCATGTTC TGCATAGACG AAGAAAACTT TGGACAGACA
    TATCAAGTGG ATTTGAAGCC CAATGGGTCA GAAATAATGG TCACAAATGA AAACAAAAGG
    GAATATATCG ACTTAGTCAT CCAGTGGAGA TTTGTGAACA GGGTCCAGAA GCAGATGAAC
    GCCTTCTTGG AGGGATTCAC AGAACTACTT CCTATTGATT TGATTAAAAT TTTTGATGAA
    AATGAGCTGG AGTTGCTCAT GTGCGGCCTC GGTGATGTGG ATGTGAATGA CTGGAGACAG
    CATTCTATTT ACAAGAACGG CTACTGCCCA AACCACCCCG TCATTCAGTG GTTCTGGAAG
    GCTGTGCTAC TCATGGACGC CGAAAAGCGT ATCCGGTTAC TGCAGTTTGT CACAGGGACA
    TCGCGAGTAC CTATGAATGG ATTTGCCGAA CTTTATGGTT CCAATGGTCC TCAGCTGTTT
    ACAATAGAGC AATGGGGCAG TCCTGAGAAA CTGCCCAGAG CTCACACATG CTTTAATCGC
    CTTGACTTAC CTCCATATGA AACCTTTGAA GATTTACGAG AGAAACTTCT CATGGCCGTG
    GAAAATGCTC AAGGATTTGA AGGGGTGGAT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu87834
    Clone ID Related Accession (Same CDS sequence) XM_017025680.1 , XM_017025680.2 , XM_024451132.1
    Accession Version XM_017025680.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2253bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017025680.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGAGCGAC CCTATACATT TAAGGACTTT CTCCTCAGAC CAAGAAGTCA TAAGTCTCGA 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCAA AAAATGGAGG TCAAGATGAA
    GAAAACAGTG ACCAGAGGGA TGACATGGAG CATGGATGGG AAGTTGTTGA CTCAAATGAC
    TCGGCTTCTC AGCACCAAGA GGAACTTCCT CCTCCTCCTC TGCCTCCCGG GTGGGAAGAA
    AAAGTGGACA ATTTAGGCCG AACTTACTAT GTCAACCACA ACAACCGGAC CACTCAGTGG
    CACAGACCAA GCCTGATGGA CGTGTCCTCG GAGTCGGACA ATAACATCAG ACAGATCAAC
    CAGGAGGCAG CACACCGGCG CTTCCGCTCC CGCAGGCACA TCAGCGAAGA CTTGGAGCCC
    GAGCCCTCGG AGGGCGGGGA TGTCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAT
    ATCGCTGGAG ACTCTCTCGG TCTGGCTCTG CCCCCACCAC CGGCCTCCCC AGGATCTCGG
    ACCAGCCCTC AGGAGCTGTC AGAGGAACTA AGCAGAAGGC TTCAGATCAC TCCAGACTCC
    AATGGGGAAC AGTTCAGCTC TTTGATTCAA AGAGAACCCT CCTCAAGGTT GAGGTCATGC
    AGTGTCACCG ACGCAGTTGC AGAACAGGGC CATCTACCAC CGGGTGCCAA GGACTCACCC
    GTACGTCGGG CTGTGAAAGA CACCCTTTCC AACCCACAGT CCCCACAGCC ATCACCTTAC
    AACTCCCCCA AACCACAACA CAAAGTCACA CAGAGCTTCT TGCCACCCGG CTGGGAAATG
    AGGATAGCGC CAAACGGCCG GCCCTTCTTC ATTGATCATA ACACAAAGAC TACAACCTGG
    GAAGATCCAC GTTTGAAATT TCCAGTACAT ATGCGGTCAA AGACATCTTT AAACCCCAAT
    GACCTTGGCC CCCTTCCTCC TGGCTGGGAA GAAAGAATTC ACTTGGATGG CCGAACGTTT
    TATATTGATC ATAATAGCAA AATTACTCAG TGGGAAGACC CAAGACTGCA GAACCCAGCT
    ATTACTGGTC CGGCTGTCCC TTACTCCAGA GAATTTAAGC AGAAATATGA CTACTTCAGG
    AAGAAATTAA AGAAACCTGC TGATATCCCC AATAGGTTTG AAATGAAACT TCACAGAAAT
    AACATATTTG AAGAGTCCTA TCGGAGAATT ATGTCCGTGA AAAGACCAGA TGTCCTAAAA
    GCTAGACTGT GGATTGAGTT TGAATCAGAG AAAGGTCTTG ACTATGGGGG TGTGGCCAGA
    GAATGGTTCT TCTTACTGTC CAAAGAGATG TTCAACCCCT ACTACGGCCT CTTTGAGTAC
    TCTGCCACGG ACAACTACAC CCTTCAGATC AACCCTAATT CAGGCCTCTG TAATGAGGAT
    CATTTGTCCT ACTTCACTTT TATTGGAAGA GTTGCTGGTC TGGCCGTATT TCATGGGAAG
    CTCTTAGATG GTTTCTTCAT TAGACCATTT TACAAGATGA TGTTGGGAAA GCAGATAACC
    CTGAATGACA TGGAATCTGT GGATAGTGAA TATTACAACT CTTTGAAATG GATCCTGGAG
    AATGACCCTA CTGAGCTGGA CCTCATGTTC TGCATAGACG AAGAAAACTT TGGACAGACA
    TATCAAGTGG ATTTGAAGCC CAATGGGTCA GAAATAATGG TCACAAATGA AAACAAAAGG
    GAATATATCG ACTTAGTCAT CCAGTGGAGA TTTGTGAACA GGGTCCAGAA GCAGATGAAC
    GCCTTCTTGG AGGGATTCAC AGAACTACTT CCTATTGATT TGATTAAAAT TTTTGATGAA
    AATGAGCTGG AGTTGCTCAT GTGCGGCCTC GGTGATGTGG ATGTGAATGA CTGGAGACAG
    CATTCTATTT ACAAGAACGG CTACTGCCCA AACCACCCCG TCATTCAGTG GTTCTGGAAG
    GCTGTGCTAC TCATGGACGC CGAAAAGCGT ATCCGGTTAC TGCAGTTTGT CACAGGGACA
    TCGCGAGTAC CTATGAATGG ATTTGCCGAA CTTTATGGTT CCAATGGTCC TCAGCTGTTT
    ACAATAGAGC AATGGGGCAG TCCTGAGAAA CTGCCCAGAG CTCACACATG CTTTAATCGC
    CTTGACTTAC CTCCATATGA AACCTTTGAA GATTTACGAG AGAAACTTCT CATGGCCGTG
    GAAAATGCTC AAGGATTTGA AGGGGTGGAT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016881169.1
    CDS586..2838
    Translation

    Target ORF information:

    RefSeq Version XM_017025680.2
    Organism Homo sapiens(human)
    Definition Homo sapiens NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant X24, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017025680.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGAGCGAC CCTATACATT TAAGGACTTT CTCCTCAGAC CAAGAAGTCA TAAGTCTCGA 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCAA AAAATGGAGG TCAAGATGAA
    GAAAACAGTG ACCAGAGGGA TGACATGGAG CATGGATGGG AAGTTGTTGA CTCAAATGAC
    TCGGCTTCTC AGCACCAAGA GGAACTTCCT CCTCCTCCTC TGCCTCCCGG GTGGGAAGAA
    AAAGTGGACA ATTTAGGCCG AACTTACTAT GTCAACCACA ACAACCGGAC CACTCAGTGG
    CACAGACCAA GCCTGATGGA CGTGTCCTCG GAGTCGGACA ATAACATCAG ACAGATCAAC
    CAGGAGGCAG CACACCGGCG CTTCCGCTCC CGCAGGCACA TCAGCGAAGA CTTGGAGCCC
    GAGCCCTCGG AGGGCGGGGA TGTCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAT
    ATCGCTGGAG ACTCTCTCGG TCTGGCTCTG CCCCCACCAC CGGCCTCCCC AGGATCTCGG
    ACCAGCCCTC AGGAGCTGTC AGAGGAACTA AGCAGAAGGC TTCAGATCAC TCCAGACTCC
    AATGGGGAAC AGTTCAGCTC TTTGATTCAA AGAGAACCCT CCTCAAGGTT GAGGTCATGC
    AGTGTCACCG ACGCAGTTGC AGAACAGGGC CATCTACCAC CGGGTGCCAA GGACTCACCC
    GTACGTCGGG CTGTGAAAGA CACCCTTTCC AACCCACAGT CCCCACAGCC ATCACCTTAC
    AACTCCCCCA AACCACAACA CAAAGTCACA CAGAGCTTCT TGCCACCCGG CTGGGAAATG
    AGGATAGCGC CAAACGGCCG GCCCTTCTTC ATTGATCATA ACACAAAGAC TACAACCTGG
    GAAGATCCAC GTTTGAAATT TCCAGTACAT ATGCGGTCAA AGACATCTTT AAACCCCAAT
    GACCTTGGCC CCCTTCCTCC TGGCTGGGAA GAAAGAATTC ACTTGGATGG CCGAACGTTT
    TATATTGATC ATAATAGCAA AATTACTCAG TGGGAAGACC CAAGACTGCA GAACCCAGCT
    ATTACTGGTC CGGCTGTCCC TTACTCCAGA GAATTTAAGC AGAAATATGA CTACTTCAGG
    AAGAAATTAA AGAAACCTGC TGATATCCCC AATAGGTTTG AAATGAAACT TCACAGAAAT
    AACATATTTG AAGAGTCCTA TCGGAGAATT ATGTCCGTGA AAAGACCAGA TGTCCTAAAA
    GCTAGACTGT GGATTGAGTT TGAATCAGAG AAAGGTCTTG ACTATGGGGG TGTGGCCAGA
    GAATGGTTCT TCTTACTGTC CAAAGAGATG TTCAACCCCT ACTACGGCCT CTTTGAGTAC
    TCTGCCACGG ACAACTACAC CCTTCAGATC AACCCTAATT CAGGCCTCTG TAATGAGGAT
    CATTTGTCCT ACTTCACTTT TATTGGAAGA GTTGCTGGTC TGGCCGTATT TCATGGGAAG
    CTCTTAGATG GTTTCTTCAT TAGACCATTT TACAAGATGA TGTTGGGAAA GCAGATAACC
    CTGAATGACA TGGAATCTGT GGATAGTGAA TATTACAACT CTTTGAAATG GATCCTGGAG
    AATGACCCTA CTGAGCTGGA CCTCATGTTC TGCATAGACG AAGAAAACTT TGGACAGACA
    TATCAAGTGG ATTTGAAGCC CAATGGGTCA GAAATAATGG TCACAAATGA AAACAAAAGG
    GAATATATCG ACTTAGTCAT CCAGTGGAGA TTTGTGAACA GGGTCCAGAA GCAGATGAAC
    GCCTTCTTGG AGGGATTCAC AGAACTACTT CCTATTGATT TGATTAAAAT TTTTGATGAA
    AATGAGCTGG AGTTGCTCAT GTGCGGCCTC GGTGATGTGG ATGTGAATGA CTGGAGACAG
    CATTCTATTT ACAAGAACGG CTACTGCCCA AACCACCCCG TCATTCAGTG GTTCTGGAAG
    GCTGTGCTAC TCATGGACGC CGAAAAGCGT ATCCGGTTAC TGCAGTTTGT CACAGGGACA
    TCGCGAGTAC CTATGAATGG ATTTGCCGAA CTTTATGGTT CCAATGGTCC TCAGCTGTTT
    ACAATAGAGC AATGGGGCAG TCCTGAGAAA CTGCCCAGAG CTCACACATG CTTTAATCGC
    CTTGACTTAC CTCCATATGA AACCTTTGAA GATTTACGAG AGAAACTTCT CATGGCCGTG
    GAAAATGCTC AAGGATTTGA AGGGGTGGAT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu87834
    Clone ID Related Accession (Same CDS sequence) XM_017025680.1 , XM_017025680.2 , XM_024451132.1
    Accession Version XM_024451132.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2253bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGAGCGAC CCTATACATT TAAGGACTTT CTCCTCAGAC CAAGAAGTCA TAAGTCTCGA 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCAA AAAATGGAGG TCAAGATGAA
    GAAAACAGTG ACCAGAGGGA TGACATGGAG CATGGATGGG AAGTTGTTGA CTCAAATGAC
    TCGGCTTCTC AGCACCAAGA GGAACTTCCT CCTCCTCCTC TGCCTCCCGG GTGGGAAGAA
    AAAGTGGACA ATTTAGGCCG AACTTACTAT GTCAACCACA ACAACCGGAC CACTCAGTGG
    CACAGACCAA GCCTGATGGA CGTGTCCTCG GAGTCGGACA ATAACATCAG ACAGATCAAC
    CAGGAGGCAG CACACCGGCG CTTCCGCTCC CGCAGGCACA TCAGCGAAGA CTTGGAGCCC
    GAGCCCTCGG AGGGCGGGGA TGTCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAT
    ATCGCTGGAG ACTCTCTCGG TCTGGCTCTG CCCCCACCAC CGGCCTCCCC AGGATCTCGG
    ACCAGCCCTC AGGAGCTGTC AGAGGAACTA AGCAGAAGGC TTCAGATCAC TCCAGACTCC
    AATGGGGAAC AGTTCAGCTC TTTGATTCAA AGAGAACCCT CCTCAAGGTT GAGGTCATGC
    AGTGTCACCG ACGCAGTTGC AGAACAGGGC CATCTACCAC CGGGTGCCAA GGACTCACCC
    GTACGTCGGG CTGTGAAAGA CACCCTTTCC AACCCACAGT CCCCACAGCC ATCACCTTAC
    AACTCCCCCA AACCACAACA CAAAGTCACA CAGAGCTTCT TGCCACCCGG CTGGGAAATG
    AGGATAGCGC CAAACGGCCG GCCCTTCTTC ATTGATCATA ACACAAAGAC TACAACCTGG
    GAAGATCCAC GTTTGAAATT TCCAGTACAT ATGCGGTCAA AGACATCTTT AAACCCCAAT
    GACCTTGGCC CCCTTCCTCC TGGCTGGGAA GAAAGAATTC ACTTGGATGG CCGAACGTTT
    TATATTGATC ATAATAGCAA AATTACTCAG TGGGAAGACC CAAGACTGCA GAACCCAGCT
    ATTACTGGTC CGGCTGTCCC TTACTCCAGA GAATTTAAGC AGAAATATGA CTACTTCAGG
    AAGAAATTAA AGAAACCTGC TGATATCCCC AATAGGTTTG AAATGAAACT TCACAGAAAT
    AACATATTTG AAGAGTCCTA TCGGAGAATT ATGTCCGTGA AAAGACCAGA TGTCCTAAAA
    GCTAGACTGT GGATTGAGTT TGAATCAGAG AAAGGTCTTG ACTATGGGGG TGTGGCCAGA
    GAATGGTTCT TCTTACTGTC CAAAGAGATG TTCAACCCCT ACTACGGCCT CTTTGAGTAC
    TCTGCCACGG ACAACTACAC CCTTCAGATC AACCCTAATT CAGGCCTCTG TAATGAGGAT
    CATTTGTCCT ACTTCACTTT TATTGGAAGA GTTGCTGGTC TGGCCGTATT TCATGGGAAG
    CTCTTAGATG GTTTCTTCAT TAGACCATTT TACAAGATGA TGTTGGGAAA GCAGATAACC
    CTGAATGACA TGGAATCTGT GGATAGTGAA TATTACAACT CTTTGAAATG GATCCTGGAG
    AATGACCCTA CTGAGCTGGA CCTCATGTTC TGCATAGACG AAGAAAACTT TGGACAGACA
    TATCAAGTGG ATTTGAAGCC CAATGGGTCA GAAATAATGG TCACAAATGA AAACAAAAGG
    GAATATATCG ACTTAGTCAT CCAGTGGAGA TTTGTGAACA GGGTCCAGAA GCAGATGAAC
    GCCTTCTTGG AGGGATTCAC AGAACTACTT CCTATTGATT TGATTAAAAT TTTTGATGAA
    AATGAGCTGG AGTTGCTCAT GTGCGGCCTC GGTGATGTGG ATGTGAATGA CTGGAGACAG
    CATTCTATTT ACAAGAACGG CTACTGCCCA AACCACCCCG TCATTCAGTG GTTCTGGAAG
    GCTGTGCTAC TCATGGACGC CGAAAAGCGT ATCCGGTTAC TGCAGTTTGT CACAGGGACA
    TCGCGAGTAC CTATGAATGG ATTTGCCGAA CTTTATGGTT CCAATGGTCC TCAGCTGTTT
    ACAATAGAGC AATGGGGCAG TCCTGAGAAA CTGCCCAGAG CTCACACATG CTTTAATCGC
    CTTGACTTAC CTCCATATGA AACCTTTGAA GATTTACGAG AGAAACTTCT CATGGCCGTG
    GAAAATGCTC AAGGATTTGA AGGGGTGGAT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024306900.1
    CDS320..2572
    Translation

    Target ORF information:

    RefSeq Version XM_024451132.1
    Organism Homo sapiens(human)
    Definition Homo sapiens NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024451132.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGAGCGAC CCTATACATT TAAGGACTTT CTCCTCAGAC CAAGAAGTCA TAAGTCTCGA 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCAA AAAATGGAGG TCAAGATGAA
    GAAAACAGTG ACCAGAGGGA TGACATGGAG CATGGATGGG AAGTTGTTGA CTCAAATGAC
    TCGGCTTCTC AGCACCAAGA GGAACTTCCT CCTCCTCCTC TGCCTCCCGG GTGGGAAGAA
    AAAGTGGACA ATTTAGGCCG AACTTACTAT GTCAACCACA ACAACCGGAC CACTCAGTGG
    CACAGACCAA GCCTGATGGA CGTGTCCTCG GAGTCGGACA ATAACATCAG ACAGATCAAC
    CAGGAGGCAG CACACCGGCG CTTCCGCTCC CGCAGGCACA TCAGCGAAGA CTTGGAGCCC
    GAGCCCTCGG AGGGCGGGGA TGTCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAT
    ATCGCTGGAG ACTCTCTCGG TCTGGCTCTG CCCCCACCAC CGGCCTCCCC AGGATCTCGG
    ACCAGCCCTC AGGAGCTGTC AGAGGAACTA AGCAGAAGGC TTCAGATCAC TCCAGACTCC
    AATGGGGAAC AGTTCAGCTC TTTGATTCAA AGAGAACCCT CCTCAAGGTT GAGGTCATGC
    AGTGTCACCG ACGCAGTTGC AGAACAGGGC CATCTACCAC CGGGTGCCAA GGACTCACCC
    GTACGTCGGG CTGTGAAAGA CACCCTTTCC AACCCACAGT CCCCACAGCC ATCACCTTAC
    AACTCCCCCA AACCACAACA CAAAGTCACA CAGAGCTTCT TGCCACCCGG CTGGGAAATG
    AGGATAGCGC CAAACGGCCG GCCCTTCTTC ATTGATCATA ACACAAAGAC TACAACCTGG
    GAAGATCCAC GTTTGAAATT TCCAGTACAT ATGCGGTCAA AGACATCTTT AAACCCCAAT
    GACCTTGGCC CCCTTCCTCC TGGCTGGGAA GAAAGAATTC ACTTGGATGG CCGAACGTTT
    TATATTGATC ATAATAGCAA AATTACTCAG TGGGAAGACC CAAGACTGCA GAACCCAGCT
    ATTACTGGTC CGGCTGTCCC TTACTCCAGA GAATTTAAGC AGAAATATGA CTACTTCAGG
    AAGAAATTAA AGAAACCTGC TGATATCCCC AATAGGTTTG AAATGAAACT TCACAGAAAT
    AACATATTTG AAGAGTCCTA TCGGAGAATT ATGTCCGTGA AAAGACCAGA TGTCCTAAAA
    GCTAGACTGT GGATTGAGTT TGAATCAGAG AAAGGTCTTG ACTATGGGGG TGTGGCCAGA
    GAATGGTTCT TCTTACTGTC CAAAGAGATG TTCAACCCCT ACTACGGCCT CTTTGAGTAC
    TCTGCCACGG ACAACTACAC CCTTCAGATC AACCCTAATT CAGGCCTCTG TAATGAGGAT
    CATTTGTCCT ACTTCACTTT TATTGGAAGA GTTGCTGGTC TGGCCGTATT TCATGGGAAG
    CTCTTAGATG GTTTCTTCAT TAGACCATTT TACAAGATGA TGTTGGGAAA GCAGATAACC
    CTGAATGACA TGGAATCTGT GGATAGTGAA TATTACAACT CTTTGAAATG GATCCTGGAG
    AATGACCCTA CTGAGCTGGA CCTCATGTTC TGCATAGACG AAGAAAACTT TGGACAGACA
    TATCAAGTGG ATTTGAAGCC CAATGGGTCA GAAATAATGG TCACAAATGA AAACAAAAGG
    GAATATATCG ACTTAGTCAT CCAGTGGAGA TTTGTGAACA GGGTCCAGAA GCAGATGAAC
    GCCTTCTTGG AGGGATTCAC AGAACTACTT CCTATTGATT TGATTAAAAT TTTTGATGAA
    AATGAGCTGG AGTTGCTCAT GTGCGGCCTC GGTGATGTGG ATGTGAATGA CTGGAGACAG
    CATTCTATTT ACAAGAACGG CTACTGCCCA AACCACCCCG TCATTCAGTG GTTCTGGAAG
    GCTGTGCTAC TCATGGACGC CGAAAAGCGT ATCCGGTTAC TGCAGTTTGT CACAGGGACA
    TCGCGAGTAC CTATGAATGG ATTTGCCGAA CTTTATGGTT CCAATGGTCC TCAGCTGTTT
    ACAATAGAGC AATGGGGCAG TCCTGAGAAA CTGCCCAGAG CTCACACATG CTTTAATCGC
    CTTGACTTAC CTCCATATGA AACCTTTGAA GATTTACGAG AGAAACTTCT CATGGCCGTG
    GAAAATGCTC AAGGATTTGA AGGGGTGGAT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu87835
    Clone ID Related Accession (Same CDS sequence) XM_017025681.1 , XM_017025681.2
    Accession Version XM_017025681.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2055bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGAGCGAC CCTATACATT TAAGGACTTT CTCCTCAGAC CAAGAAGTCA TAAGTCTCGA 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCAA AAAATGGAGG TCAAGATGAA
    GAAAACAGTG ACCAGAGGGA TGACATGGAG CATGGATGGG AAGTTGTTGA CTCAAATGAC
    TCGGCTTCTC AGCACCAAGA GGAACTTCCT CCTCCTCCTC TGCCTCCCGG GTGGGAAGAA
    AAAGTGGACA ATTTAGGCCG AACTTACTAT GTCAACCACA ACAACCGGAC CACTCAGTGG
    CACAGACCAA GCCTGATGGA CGTGTCCTCG GAGTCGGACA ATAACATCAG ACAGATCAAC
    CAGGAGGCAG CACACCGGCG CTTCCGCTCC CGCAGGCACA TCAGCGAAGA CTTGGAGCCC
    GAGCCCTCGG AGGGCGGGGA TGTCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAT
    ATCGCTGGAG ACTCTCTCGG TCTGGCTCTG CCCCCACCAC CGGCCTCCCC AGGATCTCGG
    ACCAGCCCTC AGGAGCTGTC AGAGGAACTA AGCAGAAGGC TTCAGATCAC TCCAGACTCC
    AATGGGGAAC AGTTCAGCTC TTTGATTCAA AGAGAACCCT CCTCAAGGTT GAGGTCATGC
    AGTGTCACCG ACGCAGTTGC AGAACAGGGC CATCTACCAC CGGAAGATCC ACGTTTGAAA
    TTTCCAGTAC ATATGCGGTC AAAGACATCT TTAAACCCCA ATGACCTTGG CCCCCTTCCT
    CCTGGCTGGG AAGAAAGAAT TCACTTGGAT GGCCGAACGT TTTATATTGA TCATAATAGC
    AAAATTACTC AGTGGGAAGA CCCAAGACTG CAGAACCCAG CTATTACTGG TCCGGCTGTC
    CCTTACTCCA GAGAATTTAA GCAGAAATAT GACTACTTCA GGAAGAAATT AAAGAAACCT
    GCTGATATCC CCAATAGGTT TGAAATGAAA CTTCACAGAA ATAACATATT TGAAGAGTCC
    TATCGGAGAA TTATGTCCGT GAAAAGACCA GATGTCCTAA AAGCTAGACT GTGGATTGAG
    TTTGAATCAG AGAAAGGTCT TGACTATGGG GGTGTGGCCA GAGAATGGTT CTTCTTACTG
    TCCAAAGAGA TGTTCAACCC CTACTACGGC CTCTTTGAGT ACTCTGCCAC GGACAACTAC
    ACCCTTCAGA TCAACCCTAA TTCAGGCCTC TGTAATGAGG ATCATTTGTC CTACTTCACT
    TTTATTGGAA GAGTTGCTGG TCTGGCCGTA TTTCATGGGA AGCTCTTAGA TGGTTTCTTC
    ATTAGACCAT TTTACAAGAT GATGTTGGGA AAGCAGATAA CCCTGAATGA CATGGAATCT
    GTGGATAGTG AATATTACAA CTCTTTGAAA TGGATCCTGG AGAATGACCC TACTGAGCTG
    GACCTCATGT TCTGCATAGA CGAAGAAAAC TTTGGACAGA CATATCAAGT GGATTTGAAG
    CCCAATGGGT CAGAAATAAT GGTCACAAAT GAAAACAAAA GGGAATATAT CGACTTAGTC
    ATCCAGTGGA GATTTGTGAA CAGGGTCCAG AAGCAGATGA ACGCCTTCTT GGAGGGATTC
    ACAGAACTAC TTCCTATTGA TTTGATTAAA ATTTTTGATG AAAATGAGCT GGAGTTGCTC
    ATGTGCGGCC TCGGTGATGT GGATGTGAAT GACTGGAGAC AGCATTCTAT TTACAAGAAC
    GGCTACTGCC CAAACCACCC CGTCATTCAG TGGTTCTGGA AGGCTGTGCT ACTCATGGAC
    GCCGAAAAGC GTATCCGGTT ACTGCAGTTT GTCACAGGGA CATCGCGAGT ACCTATGAAT
    GGATTTGCCG AACTTTATGG TTCCAATGGT CCTCAGCTGT TTACAATAGA GCAATGGGGC
    AGTCCTGAGA AACTGCCCAG AGCTCACACA TGCTTTAATC GCCTTGACTT ACCTCCATAT
    GAAACCTTTG AAGATTTACG AGAGAAACTT CTCATGGCCG TGGAAAATGC TCAAGGATTT
    GAAGGGGTGG ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016881170.1
    CDS571..2625
    Translation

    Target ORF information:

    RefSeq Version XM_017025681.1
    Organism Homo sapiens(human)
    Definition Homo sapiens neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017025681.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGAGCGAC CCTATACATT TAAGGACTTT CTCCTCAGAC CAAGAAGTCA TAAGTCTCGA 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCAA AAAATGGAGG TCAAGATGAA
    GAAAACAGTG ACCAGAGGGA TGACATGGAG CATGGATGGG AAGTTGTTGA CTCAAATGAC
    TCGGCTTCTC AGCACCAAGA GGAACTTCCT CCTCCTCCTC TGCCTCCCGG GTGGGAAGAA
    AAAGTGGACA ATTTAGGCCG AACTTACTAT GTCAACCACA ACAACCGGAC CACTCAGTGG
    CACAGACCAA GCCTGATGGA CGTGTCCTCG GAGTCGGACA ATAACATCAG ACAGATCAAC
    CAGGAGGCAG CACACCGGCG CTTCCGCTCC CGCAGGCACA TCAGCGAAGA CTTGGAGCCC
    GAGCCCTCGG AGGGCGGGGA TGTCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAT
    ATCGCTGGAG ACTCTCTCGG TCTGGCTCTG CCCCCACCAC CGGCCTCCCC AGGATCTCGG
    ACCAGCCCTC AGGAGCTGTC AGAGGAACTA AGCAGAAGGC TTCAGATCAC TCCAGACTCC
    AATGGGGAAC AGTTCAGCTC TTTGATTCAA AGAGAACCCT CCTCAAGGTT GAGGTCATGC
    AGTGTCACCG ACGCAGTTGC AGAACAGGGC CATCTACCAC CGGAAGATCC ACGTTTGAAA
    TTTCCAGTAC ATATGCGGTC AAAGACATCT TTAAACCCCA ATGACCTTGG CCCCCTTCCT
    CCTGGCTGGG AAGAAAGAAT TCACTTGGAT GGCCGAACGT TTTATATTGA TCATAATAGC
    AAAATTACTC AGTGGGAAGA CCCAAGACTG CAGAACCCAG CTATTACTGG TCCGGCTGTC
    CCTTACTCCA GAGAATTTAA GCAGAAATAT GACTACTTCA GGAAGAAATT AAAGAAACCT
    GCTGATATCC CCAATAGGTT TGAAATGAAA CTTCACAGAA ATAACATATT TGAAGAGTCC
    TATCGGAGAA TTATGTCCGT GAAAAGACCA GATGTCCTAA AAGCTAGACT GTGGATTGAG
    TTTGAATCAG AGAAAGGTCT TGACTATGGG GGTGTGGCCA GAGAATGGTT CTTCTTACTG
    TCCAAAGAGA TGTTCAACCC CTACTACGGC CTCTTTGAGT ACTCTGCCAC GGACAACTAC
    ACCCTTCAGA TCAACCCTAA TTCAGGCCTC TGTAATGAGG ATCATTTGTC CTACTTCACT
    TTTATTGGAA GAGTTGCTGG TCTGGCCGTA TTTCATGGGA AGCTCTTAGA TGGTTTCTTC
    ATTAGACCAT TTTACAAGAT GATGTTGGGA AAGCAGATAA CCCTGAATGA CATGGAATCT
    GTGGATAGTG AATATTACAA CTCTTTGAAA TGGATCCTGG AGAATGACCC TACTGAGCTG
    GACCTCATGT TCTGCATAGA CGAAGAAAAC TTTGGACAGA CATATCAAGT GGATTTGAAG
    CCCAATGGGT CAGAAATAAT GGTCACAAAT GAAAACAAAA GGGAATATAT CGACTTAGTC
    ATCCAGTGGA GATTTGTGAA CAGGGTCCAG AAGCAGATGA ACGCCTTCTT GGAGGGATTC
    ACAGAACTAC TTCCTATTGA TTTGATTAAA ATTTTTGATG AAAATGAGCT GGAGTTGCTC
    ATGTGCGGCC TCGGTGATGT GGATGTGAAT GACTGGAGAC AGCATTCTAT TTACAAGAAC
    GGCTACTGCC CAAACCACCC CGTCATTCAG TGGTTCTGGA AGGCTGTGCT ACTCATGGAC
    GCCGAAAAGC GTATCCGGTT ACTGCAGTTT GTCACAGGGA CATCGCGAGT ACCTATGAAT
    GGATTTGCCG AACTTTATGG TTCCAATGGT CCTCAGCTGT TTACAATAGA GCAATGGGGC
    AGTCCTGAGA AACTGCCCAG AGCTCACACA TGCTTTAATC GCCTTGACTT ACCTCCATAT
    GAAACCTTTG AAGATTTACG AGAGAAACTT CTCATGGCCG TGGAAAATGC TCAAGGATTT
    GAAGGGGTGG ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu87835
    Clone ID Related Accession (Same CDS sequence) XM_017025681.1 , XM_017025681.2
    Accession Version XM_017025681.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2055bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X18
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017025681.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGAGCGAC CCTATACATT TAAGGACTTT CTCCTCAGAC CAAGAAGTCA TAAGTCTCGA 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCAA AAAATGGAGG TCAAGATGAA
    GAAAACAGTG ACCAGAGGGA TGACATGGAG CATGGATGGG AAGTTGTTGA CTCAAATGAC
    TCGGCTTCTC AGCACCAAGA GGAACTTCCT CCTCCTCCTC TGCCTCCCGG GTGGGAAGAA
    AAAGTGGACA ATTTAGGCCG AACTTACTAT GTCAACCACA ACAACCGGAC CACTCAGTGG
    CACAGACCAA GCCTGATGGA CGTGTCCTCG GAGTCGGACA ATAACATCAG ACAGATCAAC
    CAGGAGGCAG CACACCGGCG CTTCCGCTCC CGCAGGCACA TCAGCGAAGA CTTGGAGCCC
    GAGCCCTCGG AGGGCGGGGA TGTCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAT
    ATCGCTGGAG ACTCTCTCGG TCTGGCTCTG CCCCCACCAC CGGCCTCCCC AGGATCTCGG
    ACCAGCCCTC AGGAGCTGTC AGAGGAACTA AGCAGAAGGC TTCAGATCAC TCCAGACTCC
    AATGGGGAAC AGTTCAGCTC TTTGATTCAA AGAGAACCCT CCTCAAGGTT GAGGTCATGC
    AGTGTCACCG ACGCAGTTGC AGAACAGGGC CATCTACCAC CGGAAGATCC ACGTTTGAAA
    TTTCCAGTAC ATATGCGGTC AAAGACATCT TTAAACCCCA ATGACCTTGG CCCCCTTCCT
    CCTGGCTGGG AAGAAAGAAT TCACTTGGAT GGCCGAACGT TTTATATTGA TCATAATAGC
    AAAATTACTC AGTGGGAAGA CCCAAGACTG CAGAACCCAG CTATTACTGG TCCGGCTGTC
    CCTTACTCCA GAGAATTTAA GCAGAAATAT GACTACTTCA GGAAGAAATT AAAGAAACCT
    GCTGATATCC CCAATAGGTT TGAAATGAAA CTTCACAGAA ATAACATATT TGAAGAGTCC
    TATCGGAGAA TTATGTCCGT GAAAAGACCA GATGTCCTAA AAGCTAGACT GTGGATTGAG
    TTTGAATCAG AGAAAGGTCT TGACTATGGG GGTGTGGCCA GAGAATGGTT CTTCTTACTG
    TCCAAAGAGA TGTTCAACCC CTACTACGGC CTCTTTGAGT ACTCTGCCAC GGACAACTAC
    ACCCTTCAGA TCAACCCTAA TTCAGGCCTC TGTAATGAGG ATCATTTGTC CTACTTCACT
    TTTATTGGAA GAGTTGCTGG TCTGGCCGTA TTTCATGGGA AGCTCTTAGA TGGTTTCTTC
    ATTAGACCAT TTTACAAGAT GATGTTGGGA AAGCAGATAA CCCTGAATGA CATGGAATCT
    GTGGATAGTG AATATTACAA CTCTTTGAAA TGGATCCTGG AGAATGACCC TACTGAGCTG
    GACCTCATGT TCTGCATAGA CGAAGAAAAC TTTGGACAGA CATATCAAGT GGATTTGAAG
    CCCAATGGGT CAGAAATAAT GGTCACAAAT GAAAACAAAA GGGAATATAT CGACTTAGTC
    ATCCAGTGGA GATTTGTGAA CAGGGTCCAG AAGCAGATGA ACGCCTTCTT GGAGGGATTC
    ACAGAACTAC TTCCTATTGA TTTGATTAAA ATTTTTGATG AAAATGAGCT GGAGTTGCTC
    ATGTGCGGCC TCGGTGATGT GGATGTGAAT GACTGGAGAC AGCATTCTAT TTACAAGAAC
    GGCTACTGCC CAAACCACCC CGTCATTCAG TGGTTCTGGA AGGCTGTGCT ACTCATGGAC
    GCCGAAAAGC GTATCCGGTT ACTGCAGTTT GTCACAGGGA CATCGCGAGT ACCTATGAAT
    GGATTTGCCG AACTTTATGG TTCCAATGGT CCTCAGCTGT TTACAATAGA GCAATGGGGC
    AGTCCTGAGA AACTGCCCAG AGCTCACACA TGCTTTAATC GCCTTGACTT ACCTCCATAT
    GAAACCTTTG AAGATTTACG AGAGAAACTT CTCATGGCCG TGGAAAATGC TCAAGGATTT
    GAAGGGGTGG ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016881170.1
    CDS586..2640
    Translation

    Target ORF information:

    RefSeq Version XM_017025681.2
    Organism Homo sapiens(human)
    Definition Homo sapiens NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant X25, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017025681.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGAGCGAC CCTATACATT TAAGGACTTT CTCCTCAGAC CAAGAAGTCA TAAGTCTCGA 
    GTTAAGGGAT TTTTGCGATT GAAAATGGCC TATATGCCAA AAAATGGAGG TCAAGATGAA
    GAAAACAGTG ACCAGAGGGA TGACATGGAG CATGGATGGG AAGTTGTTGA CTCAAATGAC
    TCGGCTTCTC AGCACCAAGA GGAACTTCCT CCTCCTCCTC TGCCTCCCGG GTGGGAAGAA
    AAAGTGGACA ATTTAGGCCG AACTTACTAT GTCAACCACA ACAACCGGAC CACTCAGTGG
    CACAGACCAA GCCTGATGGA CGTGTCCTCG GAGTCGGACA ATAACATCAG ACAGATCAAC
    CAGGAGGCAG CACACCGGCG CTTCCGCTCC CGCAGGCACA TCAGCGAAGA CTTGGAGCCC
    GAGCCCTCGG AGGGCGGGGA TGTCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAT
    ATCGCTGGAG ACTCTCTCGG TCTGGCTCTG CCCCCACCAC CGGCCTCCCC AGGATCTCGG
    ACCAGCCCTC AGGAGCTGTC AGAGGAACTA AGCAGAAGGC TTCAGATCAC TCCAGACTCC
    AATGGGGAAC AGTTCAGCTC TTTGATTCAA AGAGAACCCT CCTCAAGGTT GAGGTCATGC
    AGTGTCACCG ACGCAGTTGC AGAACAGGGC CATCTACCAC CGGAAGATCC ACGTTTGAAA
    TTTCCAGTAC ATATGCGGTC AAAGACATCT TTAAACCCCA ATGACCTTGG CCCCCTTCCT
    CCTGGCTGGG AAGAAAGAAT TCACTTGGAT GGCCGAACGT TTTATATTGA TCATAATAGC
    AAAATTACTC AGTGGGAAGA CCCAAGACTG CAGAACCCAG CTATTACTGG TCCGGCTGTC
    CCTTACTCCA GAGAATTTAA GCAGAAATAT GACTACTTCA GGAAGAAATT AAAGAAACCT
    GCTGATATCC CCAATAGGTT TGAAATGAAA CTTCACAGAA ATAACATATT TGAAGAGTCC
    TATCGGAGAA TTATGTCCGT GAAAAGACCA GATGTCCTAA AAGCTAGACT GTGGATTGAG
    TTTGAATCAG AGAAAGGTCT TGACTATGGG GGTGTGGCCA GAGAATGGTT CTTCTTACTG
    TCCAAAGAGA TGTTCAACCC CTACTACGGC CTCTTTGAGT ACTCTGCCAC GGACAACTAC
    ACCCTTCAGA TCAACCCTAA TTCAGGCCTC TGTAATGAGG ATCATTTGTC CTACTTCACT
    TTTATTGGAA GAGTTGCTGG TCTGGCCGTA TTTCATGGGA AGCTCTTAGA TGGTTTCTTC
    ATTAGACCAT TTTACAAGAT GATGTTGGGA AAGCAGATAA CCCTGAATGA CATGGAATCT
    GTGGATAGTG AATATTACAA CTCTTTGAAA TGGATCCTGG AGAATGACCC TACTGAGCTG
    GACCTCATGT TCTGCATAGA CGAAGAAAAC TTTGGACAGA CATATCAAGT GGATTTGAAG
    CCCAATGGGT CAGAAATAAT GGTCACAAAT GAAAACAAAA GGGAATATAT CGACTTAGTC
    ATCCAGTGGA GATTTGTGAA CAGGGTCCAG AAGCAGATGA ACGCCTTCTT GGAGGGATTC
    ACAGAACTAC TTCCTATTGA TTTGATTAAA ATTTTTGATG AAAATGAGCT GGAGTTGCTC
    ATGTGCGGCC TCGGTGATGT GGATGTGAAT GACTGGAGAC AGCATTCTAT TTACAAGAAC
    GGCTACTGCC CAAACCACCC CGTCATTCAG TGGTTCTGGA AGGCTGTGCT ACTCATGGAC
    GCCGAAAAGC GTATCCGGTT ACTGCAGTTT GTCACAGGGA CATCGCGAGT ACCTATGAAT
    GGATTTGCCG AACTTTATGG TTCCAATGGT CCTCAGCTGT TTACAATAGA GCAATGGGGC
    AGTCCTGAGA AACTGCCCAG AGCTCACACA TGCTTTAATC GCCTTGACTT ACCTCCATAT
    GAAACCTTTG AAGATTTACG AGAGAAACTT CTCATGGCCG TGGAAAATGC TCAAGGATTT
    GAAGGGGTGG ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu51144
    Clone ID Related Accession (Same CDS sequence) XM_006722426.3 , XM_006722426.4
    Accession Version XM_006722426.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3060bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006722426.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGCGACCG GGCTCGGGGA GCCGGTCTAT GGACTTTCCG AAGACGAGGG AGAGTCCCGT 
    ATTCTCAGAG TAAAAGTTGT TTCTGGAATT GATCTCGCCA AAAAGGACAT CTTTGGAGCC
    AGCTTCTGCT CCATTGCTGT TGAACTTTTT TTTGGTCGTG ACATCAGAAA AATAACCAAG
    TGTGAGTCTG ATAATAGCAA TGATCCGTAT GTGAAACTTT CATTGTACGT AGCGGATGAG
    AATAGAGAAC TTGCTTTGGT CCAGACAAAA ACAATTAAAA AGACACTGAA CCCAAAATGG
    AATGAAGAAT TTTATTTCAG GGTAAACCCA TCTAATCACA GACTCCTATT TGAAGTATTT
    GACGAAAATA GACTGACACG AGACGACTTC CTGGGCCAGG TGGACGTGCC CCTTAGTCAC
    CTTCCGACAG AAGATCCAAC CATGGAGCGA CCCTATACAT TTAAGGACTT TCTCCTCAGA
    CCAAGAAGTC ATAAGTCTCG AGTTAAGGGA TTTTTGCGAT TGAAAATGGC CTATATGCCA
    AAAAATGGAG GTCAAGATGA AGAAAACAGT GACCAGAGGG ATGACATGGA GCATGGATGG
    GAAGTTGTTG ACTCAAATGA CTCGGCTTCT CAGCACCAAG AGGAACTTCC TCCTCCTCCT
    CTGCCTCCCG GGTGGGAAGA AAAAGTGGAC AATTTAGGCC GAACTTACTA TGTCAACCAC
    AACAACCGGA CCACTCAGTG GCACAGACCA AGCCTGATGG ACGTGTCCTC GGAGTCGGAC
    AATAACATCA GACAGATCAA CCAGGAGGCA GCACACCGGC GCTTCCGCTC CCGCAGGCAC
    ATCAGCGAAG ACTTGGAGCC CGAGCCCTCG GAGGGCGGGG ATGTCCCCGA GCCTTGGGAG
    ACCATTTCAG AGGAAGTGAA TATCGCTGGA GACTCTCTCG GTCTGGCTCT GCCCCCACCA
    CCGGCCTCCC CAGGATCTCG GACCAGCCCT CAGGAGCTGT CAGAGGAACT AAGCAGAAGG
    CTTCAGATCA CTCCAGACTC CAATGGGGAA CAGTTCAGCT CTTTGATTCA AAGAGAACCC
    TCCTCAAGGT TGAGGTCATG CAGTGTCACC GACGCAGTTG CAGAACAGGG CCATCTACCA
    CCGCCCAGTG CCCCAGCTGG GAGAGCGCGT TCATCAACTG TCACGGGTGG TGAGGAACCA
    ACGCCATCAG TGGCCTATGT ACATACCACG CCGGGTCTGC CTTCAGGCTG GGAAGAAAGA
    AAAGATGCTA AGGGGCGCAC ATACTATGTC AATCATAACA ATCGAACCAC AACTTGGACT
    CGACCTATCA TGCAGCTTGC AGAAGATGGT GCGTCCGGAT CAGCCACAAA CAGTAACAAC
    CATCTAATCG AGCCTCAGAT CCGCCGGCCT CGTAGCCTCA GCTCGCCAAC AGTAACTTTA
    TCTGCCCCGC TGGAGGGTGC CAAGGACTCA CCCGTACGTC GGGCTGTGAA AGACACCCTT
    TCCAACCCAC AGTCCCCACA GCCATCACCT TACAACTCCC CCAAACCACA ACACAAAGTC
    ACACAGAGCT TCTTGCCACC CGGCTGGGAA ATGAGGATAG CGCCAAACGG CCGGCCCTTC
    TTCATTGATC ATAACACAAA GACTACAACC TGGGAAGATC CACGTTTGAA ATTTCCAGTA
    CATATGCGGT CAAAGACATC TTTAAACCCC AATGACCTTG GCCCCCTTCC TCCTGGCTGG
    GAAGAAAGAA TTCACTTGGA TGGCCGAACG TTTTATATTG ATCATAGCAG CCAGGTGTTA
    TGTGGAGAAA ATGATACCAG AGAATCAGTG CCTTCATACG ATAGCAAAAT TACTCAGTGG
    GAAGACCCAA GACTGCAGAA CCCAGCTATT ACTGGTCCGG CTGTCCCTTA CTCCAGAGAA
    TTTAAGCAGA AATATGACTA CTTCAGGAAG AAATTAAAGA AACCTGCTGA TATCCCCAAT
    AGGTTTGAAA TGAAACTTCA CAGAAATAAC ATATTTGAAG AGTCCTATCG GAGAATTATG
    TCCGTGAAAA GACCAGATGT CCTAAAAGCT AGACTGTGGA TTGAGTTTGA ATCAGAGAAA
    GGTCTTGACT ATGGGGGTGT GGCCAGAGAA TGGTTCTTCT TACTGTCCAA AGAGATGTTC
    AACCCCTACT ACGGCCTCTT TGAGTACTCT GCCACGGACA ACTACACCCT TCAGATCAAC
    CCTAATTCAG GCCTCTGTAA TGAGGATCAT TTGTCCTACT TCACTTTTAT TGGAAGAGTT
    GCTGGTCTGG CCGTATTTCA TGGGAAGCTC TTAGATGGTT TCTTCATTAG ACCATTTTAC
    AAGATGATGT TGGGAAAGCA GATAACCCTG AATGACATGG AATCTGTGGA TAGTGAATAT
    TACAACTCTT TGAAATGGAT CCTGGAGAAT GACCCTACTG AGCTGGACCT CATGTTCTGC
    ATAGACGAAG AAAACTTTGG ACAGACATAT CAAGTGGATT TGAAGCCCAA TGGGTCAGAA
    ATAATGGTCA CAAATGAAAA CAAAAGGGAA TATATCGACT TAGTCATCCA GTGGAGATTT
    GTGAACAGGG TCCAGAAGCA GATGAACGCC TTCTTGGAGG GATTCACAGA ACTACTTCCT
    ATTGATTTGA TTAAAATTTT TGATGAAAAT GAGCTGGAGT TGCTCATGTG CGGCCTCGGT
    GATGTGGATG TGAATGACTG GAGACAGCAT TCTATTTACA AGAACGGCTA CTGCCCAAAC
    CACCCCGTCA TTCAGTGGTT CTGGAAGGCT GTGCTACTCA TGGACGCCGA AAAGCGTATC
    CGGTTACTGC AGTTTGTCAC AGGGACATCG CGAGTACCTA TGAATGGATT TGCCGAACTT
    TATGGTTCCA ATGGTCCTCA GCTGTTTACA ATAGAGCAAT GGGGCAGTCC TGAGAAACTG
    CCCAGAGCTC ACACATGCTT TAATCGCCTT GACTTACCTC CATATGAAAC CTTTGAAGAT
    TTACGAGAGA AACTTCTCAT GGCCGTGGAA AATGCTCAAG GATTTGAAGG GGTGGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006722489.1
    CDS300..3359
    Translation

    Target ORF information:

    RefSeq Version XM_006722426.3
    Organism Homo sapiens(human)
    Definition Homo sapiens neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006722426.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGCGACCG GGCTCGGGGA GCCGGTCTAT GGACTTTCCG AAGACGAGGG AGAGTCCCGT 
    ATTCTCAGAG TAAAAGTTGT TTCTGGAATT GATCTCGCCA AAAAGGACAT CTTTGGAGCC
    AGCTTCTGCT CCATTGCTGT TGAACTTTTT TTTGGTCGTG ACATCAGAAA AATAACCAAG
    TGTGAGTCTG ATAATAGCAA TGATCCGTAT GTGAAACTTT CATTGTACGT AGCGGATGAG
    AATAGAGAAC TTGCTTTGGT CCAGACAAAA ACAATTAAAA AGACACTGAA CCCAAAATGG
    AATGAAGAAT TTTATTTCAG GGTAAACCCA TCTAATCACA GACTCCTATT TGAAGTATTT
    GACGAAAATA GACTGACACG AGACGACTTC CTGGGCCAGG TGGACGTGCC CCTTAGTCAC
    CTTCCGACAG AAGATCCAAC CATGGAGCGA CCCTATACAT TTAAGGACTT TCTCCTCAGA
    CCAAGAAGTC ATAAGTCTCG AGTTAAGGGA TTTTTGCGAT TGAAAATGGC CTATATGCCA
    AAAAATGGAG GTCAAGATGA AGAAAACAGT GACCAGAGGG ATGACATGGA GCATGGATGG
    GAAGTTGTTG ACTCAAATGA CTCGGCTTCT CAGCACCAAG AGGAACTTCC TCCTCCTCCT
    CTGCCTCCCG GGTGGGAAGA AAAAGTGGAC AATTTAGGCC GAACTTACTA TGTCAACCAC
    AACAACCGGA CCACTCAGTG GCACAGACCA AGCCTGATGG ACGTGTCCTC GGAGTCGGAC
    AATAACATCA GACAGATCAA CCAGGAGGCA GCACACCGGC GCTTCCGCTC CCGCAGGCAC
    ATCAGCGAAG ACTTGGAGCC CGAGCCCTCG GAGGGCGGGG ATGTCCCCGA GCCTTGGGAG
    ACCATTTCAG AGGAAGTGAA TATCGCTGGA GACTCTCTCG GTCTGGCTCT GCCCCCACCA
    CCGGCCTCCC CAGGATCTCG GACCAGCCCT CAGGAGCTGT CAGAGGAACT AAGCAGAAGG
    CTTCAGATCA CTCCAGACTC CAATGGGGAA CAGTTCAGCT CTTTGATTCA AAGAGAACCC
    TCCTCAAGGT TGAGGTCATG CAGTGTCACC GACGCAGTTG CAGAACAGGG CCATCTACCA
    CCGCCCAGTG CCCCAGCTGG GAGAGCGCGT TCATCAACTG TCACGGGTGG TGAGGAACCA
    ACGCCATCAG TGGCCTATGT ACATACCACG CCGGGTCTGC CTTCAGGCTG GGAAGAAAGA
    AAAGATGCTA AGGGGCGCAC ATACTATGTC AATCATAACA ATCGAACCAC AACTTGGACT
    CGACCTATCA TGCAGCTTGC AGAAGATGGT GCGTCCGGAT CAGCCACAAA CAGTAACAAC
    CATCTAATCG AGCCTCAGAT CCGCCGGCCT CGTAGCCTCA GCTCGCCAAC AGTAACTTTA
    TCTGCCCCGC TGGAGGGTGC CAAGGACTCA CCCGTACGTC GGGCTGTGAA AGACACCCTT
    TCCAACCCAC AGTCCCCACA GCCATCACCT TACAACTCCC CCAAACCACA ACACAAAGTC
    ACACAGAGCT TCTTGCCACC CGGCTGGGAA ATGAGGATAG CGCCAAACGG CCGGCCCTTC
    TTCATTGATC ATAACACAAA GACTACAACC TGGGAAGATC CACGTTTGAA ATTTCCAGTA
    CATATGCGGT CAAAGACATC TTTAAACCCC AATGACCTTG GCCCCCTTCC TCCTGGCTGG
    GAAGAAAGAA TTCACTTGGA TGGCCGAACG TTTTATATTG ATCATAGCAG CCAGGTGTTA
    TGTGGAGAAA ATGATACCAG AGAATCAGTG CCTTCATACG ATAGCAAAAT TACTCAGTGG
    GAAGACCCAA GACTGCAGAA CCCAGCTATT ACTGGTCCGG CTGTCCCTTA CTCCAGAGAA
    TTTAAGCAGA AATATGACTA CTTCAGGAAG AAATTAAAGA AACCTGCTGA TATCCCCAAT
    AGGTTTGAAA TGAAACTTCA CAGAAATAAC ATATTTGAAG AGTCCTATCG GAGAATTATG
    TCCGTGAAAA GACCAGATGT CCTAAAAGCT AGACTGTGGA TTGAGTTTGA ATCAGAGAAA
    GGTCTTGACT ATGGGGGTGT GGCCAGAGAA TGGTTCTTCT TACTGTCCAA AGAGATGTTC
    AACCCCTACT ACGGCCTCTT TGAGTACTCT GCCACGGACA ACTACACCCT TCAGATCAAC
    CCTAATTCAG GCCTCTGTAA TGAGGATCAT TTGTCCTACT TCACTTTTAT TGGAAGAGTT
    GCTGGTCTGG CCGTATTTCA TGGGAAGCTC TTAGATGGTT TCTTCATTAG ACCATTTTAC
    AAGATGATGT TGGGAAAGCA GATAACCCTG AATGACATGG AATCTGTGGA TAGTGAATAT
    TACAACTCTT TGAAATGGAT CCTGGAGAAT GACCCTACTG AGCTGGACCT CATGTTCTGC
    ATAGACGAAG AAAACTTTGG ACAGACATAT CAAGTGGATT TGAAGCCCAA TGGGTCAGAA
    ATAATGGTCA CAAATGAAAA CAAAAGGGAA TATATCGACT TAGTCATCCA GTGGAGATTT
    GTGAACAGGG TCCAGAAGCA GATGAACGCC TTCTTGGAGG GATTCACAGA ACTACTTCCT
    ATTGATTTGA TTAAAATTTT TGATGAAAAT GAGCTGGAGT TGCTCATGTG CGGCCTCGGT
    GATGTGGATG TGAATGACTG GAGACAGCAT TCTATTTACA AGAACGGCTA CTGCCCAAAC
    CACCCCGTCA TTCAGTGGTT CTGGAAGGCT GTGCTACTCA TGGACGCCGA AAAGCGTATC
    CGGTTACTGC AGTTTGTCAC AGGGACATCG CGAGTACCTA TGAATGGATT TGCCGAACTT
    TATGGTTCCA ATGGTCCTCA GCTGTTTACA ATAGAGCAAT GGGGCAGTCC TGAGAAACTG
    CCCAGAGCTC ACACATGCTT TAATCGCCTT GACTTACCTC CATATGAAAC CTTTGAAGAT
    TTACGAGAGA AACTTCTCAT GGCCGTGGAA AATGCTCAAG GATTTGAAGG GGTGGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu51144
    Clone ID Related Accession (Same CDS sequence) XM_006722426.3 , XM_006722426.4
    Accession Version XM_006722426.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3060bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_006722426.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGCGACCG GGCTCGGGGA GCCGGTCTAT GGACTTTCCG AAGACGAGGG AGAGTCCCGT 
    ATTCTCAGAG TAAAAGTTGT TTCTGGAATT GATCTCGCCA AAAAGGACAT CTTTGGAGCC
    AGCTTCTGCT CCATTGCTGT TGAACTTTTT TTTGGTCGTG ACATCAGAAA AATAACCAAG
    TGTGAGTCTG ATAATAGCAA TGATCCGTAT GTGAAACTTT CATTGTACGT AGCGGATGAG
    AATAGAGAAC TTGCTTTGGT CCAGACAAAA ACAATTAAAA AGACACTGAA CCCAAAATGG
    AATGAAGAAT TTTATTTCAG GGTAAACCCA TCTAATCACA GACTCCTATT TGAAGTATTT
    GACGAAAATA GACTGACACG AGACGACTTC CTGGGCCAGG TGGACGTGCC CCTTAGTCAC
    CTTCCGACAG AAGATCCAAC CATGGAGCGA CCCTATACAT TTAAGGACTT TCTCCTCAGA
    CCAAGAAGTC ATAAGTCTCG AGTTAAGGGA TTTTTGCGAT TGAAAATGGC CTATATGCCA
    AAAAATGGAG GTCAAGATGA AGAAAACAGT GACCAGAGGG ATGACATGGA GCATGGATGG
    GAAGTTGTTG ACTCAAATGA CTCGGCTTCT CAGCACCAAG AGGAACTTCC TCCTCCTCCT
    CTGCCTCCCG GGTGGGAAGA AAAAGTGGAC AATTTAGGCC GAACTTACTA TGTCAACCAC
    AACAACCGGA CCACTCAGTG GCACAGACCA AGCCTGATGG ACGTGTCCTC GGAGTCGGAC
    AATAACATCA GACAGATCAA CCAGGAGGCA GCACACCGGC GCTTCCGCTC CCGCAGGCAC
    ATCAGCGAAG ACTTGGAGCC CGAGCCCTCG GAGGGCGGGG ATGTCCCCGA GCCTTGGGAG
    ACCATTTCAG AGGAAGTGAA TATCGCTGGA GACTCTCTCG GTCTGGCTCT GCCCCCACCA
    CCGGCCTCCC CAGGATCTCG GACCAGCCCT CAGGAGCTGT CAGAGGAACT AAGCAGAAGG
    CTTCAGATCA CTCCAGACTC CAATGGGGAA CAGTTCAGCT CTTTGATTCA AAGAGAACCC
    TCCTCAAGGT TGAGGTCATG CAGTGTCACC GACGCAGTTG CAGAACAGGG CCATCTACCA
    CCGCCCAGTG CCCCAGCTGG GAGAGCGCGT TCATCAACTG TCACGGGTGG TGAGGAACCA
    ACGCCATCAG TGGCCTATGT ACATACCACG CCGGGTCTGC CTTCAGGCTG GGAAGAAAGA
    AAAGATGCTA AGGGGCGCAC ATACTATGTC AATCATAACA ATCGAACCAC AACTTGGACT
    CGACCTATCA TGCAGCTTGC AGAAGATGGT GCGTCCGGAT CAGCCACAAA CAGTAACAAC
    CATCTAATCG AGCCTCAGAT CCGCCGGCCT CGTAGCCTCA GCTCGCCAAC AGTAACTTTA
    TCTGCCCCGC TGGAGGGTGC CAAGGACTCA CCCGTACGTC GGGCTGTGAA AGACACCCTT
    TCCAACCCAC AGTCCCCACA GCCATCACCT TACAACTCCC CCAAACCACA ACACAAAGTC
    ACACAGAGCT TCTTGCCACC CGGCTGGGAA ATGAGGATAG CGCCAAACGG CCGGCCCTTC
    TTCATTGATC ATAACACAAA GACTACAACC TGGGAAGATC CACGTTTGAA ATTTCCAGTA
    CATATGCGGT CAAAGACATC TTTAAACCCC AATGACCTTG GCCCCCTTCC TCCTGGCTGG
    GAAGAAAGAA TTCACTTGGA TGGCCGAACG TTTTATATTG ATCATAGCAG CCAGGTGTTA
    TGTGGAGAAA ATGATACCAG AGAATCAGTG CCTTCATACG ATAGCAAAAT TACTCAGTGG
    GAAGACCCAA GACTGCAGAA CCCAGCTATT ACTGGTCCGG CTGTCCCTTA CTCCAGAGAA
    TTTAAGCAGA AATATGACTA CTTCAGGAAG AAATTAAAGA AACCTGCTGA TATCCCCAAT
    AGGTTTGAAA TGAAACTTCA CAGAAATAAC ATATTTGAAG AGTCCTATCG GAGAATTATG
    TCCGTGAAAA GACCAGATGT CCTAAAAGCT AGACTGTGGA TTGAGTTTGA ATCAGAGAAA
    GGTCTTGACT ATGGGGGTGT GGCCAGAGAA TGGTTCTTCT TACTGTCCAA AGAGATGTTC
    AACCCCTACT ACGGCCTCTT TGAGTACTCT GCCACGGACA ACTACACCCT TCAGATCAAC
    CCTAATTCAG GCCTCTGTAA TGAGGATCAT TTGTCCTACT TCACTTTTAT TGGAAGAGTT
    GCTGGTCTGG CCGTATTTCA TGGGAAGCTC TTAGATGGTT TCTTCATTAG ACCATTTTAC
    AAGATGATGT TGGGAAAGCA GATAACCCTG AATGACATGG AATCTGTGGA TAGTGAATAT
    TACAACTCTT TGAAATGGAT CCTGGAGAAT GACCCTACTG AGCTGGACCT CATGTTCTGC
    ATAGACGAAG AAAACTTTGG ACAGACATAT CAAGTGGATT TGAAGCCCAA TGGGTCAGAA
    ATAATGGTCA CAAATGAAAA CAAAAGGGAA TATATCGACT TAGTCATCCA GTGGAGATTT
    GTGAACAGGG TCCAGAAGCA GATGAACGCC TTCTTGGAGG GATTCACAGA ACTACTTCCT
    ATTGATTTGA TTAAAATTTT TGATGAAAAT GAGCTGGAGT TGCTCATGTG CGGCCTCGGT
    GATGTGGATG TGAATGACTG GAGACAGCAT TCTATTTACA AGAACGGCTA CTGCCCAAAC
    CACCCCGTCA TTCAGTGGTT CTGGAAGGCT GTGCTACTCA TGGACGCCGA AAAGCGTATC
    CGGTTACTGC AGTTTGTCAC AGGGACATCG CGAGTACCTA TGAATGGATT TGCCGAACTT
    TATGGTTCCA ATGGTCCTCA GCTGTTTACA ATAGAGCAAT GGGGCAGTCC TGAGAAACTG
    CCCAGAGCTC ACACATGCTT TAATCGCCTT GACTTACCTC CATATGAAAC CTTTGAAGAT
    TTACGAGAGA AACTTCTCAT GGCCGTGGAA AATGCTCAAG GATTTGAAGG GGTGGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006722489.1
    CDS428..3487
    Translation

    Target ORF information:

    RefSeq Version XM_006722426.4
    Organism Homo sapiens(human)
    Definition Homo sapiens NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006722426.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGCGACCG GGCTCGGGGA GCCGGTCTAT GGACTTTCCG AAGACGAGGG AGAGTCCCGT 
    ATTCTCAGAG TAAAAGTTGT TTCTGGAATT GATCTCGCCA AAAAGGACAT CTTTGGAGCC
    AGCTTCTGCT CCATTGCTGT TGAACTTTTT TTTGGTCGTG ACATCAGAAA AATAACCAAG
    TGTGAGTCTG ATAATAGCAA TGATCCGTAT GTGAAACTTT CATTGTACGT AGCGGATGAG
    AATAGAGAAC TTGCTTTGGT CCAGACAAAA ACAATTAAAA AGACACTGAA CCCAAAATGG
    AATGAAGAAT TTTATTTCAG GGTAAACCCA TCTAATCACA GACTCCTATT TGAAGTATTT
    GACGAAAATA GACTGACACG AGACGACTTC CTGGGCCAGG TGGACGTGCC CCTTAGTCAC
    CTTCCGACAG AAGATCCAAC CATGGAGCGA CCCTATACAT TTAAGGACTT TCTCCTCAGA
    CCAAGAAGTC ATAAGTCTCG AGTTAAGGGA TTTTTGCGAT TGAAAATGGC CTATATGCCA
    AAAAATGGAG GTCAAGATGA AGAAAACAGT GACCAGAGGG ATGACATGGA GCATGGATGG
    GAAGTTGTTG ACTCAAATGA CTCGGCTTCT CAGCACCAAG AGGAACTTCC TCCTCCTCCT
    CTGCCTCCCG GGTGGGAAGA AAAAGTGGAC AATTTAGGCC GAACTTACTA TGTCAACCAC
    AACAACCGGA CCACTCAGTG GCACAGACCA AGCCTGATGG ACGTGTCCTC GGAGTCGGAC
    AATAACATCA GACAGATCAA CCAGGAGGCA GCACACCGGC GCTTCCGCTC CCGCAGGCAC
    ATCAGCGAAG ACTTGGAGCC CGAGCCCTCG GAGGGCGGGG ATGTCCCCGA GCCTTGGGAG
    ACCATTTCAG AGGAAGTGAA TATCGCTGGA GACTCTCTCG GTCTGGCTCT GCCCCCACCA
    CCGGCCTCCC CAGGATCTCG GACCAGCCCT CAGGAGCTGT CAGAGGAACT AAGCAGAAGG
    CTTCAGATCA CTCCAGACTC CAATGGGGAA CAGTTCAGCT CTTTGATTCA AAGAGAACCC
    TCCTCAAGGT TGAGGTCATG CAGTGTCACC GACGCAGTTG CAGAACAGGG CCATCTACCA
    CCGCCCAGTG CCCCAGCTGG GAGAGCGCGT TCATCAACTG TCACGGGTGG TGAGGAACCA
    ACGCCATCAG TGGCCTATGT ACATACCACG CCGGGTCTGC CTTCAGGCTG GGAAGAAAGA
    AAAGATGCTA AGGGGCGCAC ATACTATGTC AATCATAACA ATCGAACCAC AACTTGGACT
    CGACCTATCA TGCAGCTTGC AGAAGATGGT GCGTCCGGAT CAGCCACAAA CAGTAACAAC
    CATCTAATCG AGCCTCAGAT CCGCCGGCCT CGTAGCCTCA GCTCGCCAAC AGTAACTTTA
    TCTGCCCCGC TGGAGGGTGC CAAGGACTCA CCCGTACGTC GGGCTGTGAA AGACACCCTT
    TCCAACCCAC AGTCCCCACA GCCATCACCT TACAACTCCC CCAAACCACA ACACAAAGTC
    ACACAGAGCT TCTTGCCACC CGGCTGGGAA ATGAGGATAG CGCCAAACGG CCGGCCCTTC
    TTCATTGATC ATAACACAAA GACTACAACC TGGGAAGATC CACGTTTGAA ATTTCCAGTA
    CATATGCGGT CAAAGACATC TTTAAACCCC AATGACCTTG GCCCCCTTCC TCCTGGCTGG
    GAAGAAAGAA TTCACTTGGA TGGCCGAACG TTTTATATTG ATCATAGCAG CCAGGTGTTA
    TGTGGAGAAA ATGATACCAG AGAATCAGTG CCTTCATACG ATAGCAAAAT TACTCAGTGG
    GAAGACCCAA GACTGCAGAA CCCAGCTATT ACTGGTCCGG CTGTCCCTTA CTCCAGAGAA
    TTTAAGCAGA AATATGACTA CTTCAGGAAG AAATTAAAGA AACCTGCTGA TATCCCCAAT
    AGGTTTGAAA TGAAACTTCA CAGAAATAAC ATATTTGAAG AGTCCTATCG GAGAATTATG
    TCCGTGAAAA GACCAGATGT CCTAAAAGCT AGACTGTGGA TTGAGTTTGA ATCAGAGAAA
    GGTCTTGACT ATGGGGGTGT GGCCAGAGAA TGGTTCTTCT TACTGTCCAA AGAGATGTTC
    AACCCCTACT ACGGCCTCTT TGAGTACTCT GCCACGGACA ACTACACCCT TCAGATCAAC
    CCTAATTCAG GCCTCTGTAA TGAGGATCAT TTGTCCTACT TCACTTTTAT TGGAAGAGTT
    GCTGGTCTGG CCGTATTTCA TGGGAAGCTC TTAGATGGTT TCTTCATTAG ACCATTTTAC
    AAGATGATGT TGGGAAAGCA GATAACCCTG AATGACATGG AATCTGTGGA TAGTGAATAT
    TACAACTCTT TGAAATGGAT CCTGGAGAAT GACCCTACTG AGCTGGACCT CATGTTCTGC
    ATAGACGAAG AAAACTTTGG ACAGACATAT CAAGTGGATT TGAAGCCCAA TGGGTCAGAA
    ATAATGGTCA CAAATGAAAA CAAAAGGGAA TATATCGACT TAGTCATCCA GTGGAGATTT
    GTGAACAGGG TCCAGAAGCA GATGAACGCC TTCTTGGAGG GATTCACAGA ACTACTTCCT
    ATTGATTTGA TTAAAATTTT TGATGAAAAT GAGCTGGAGT TGCTCATGTG CGGCCTCGGT
    GATGTGGATG TGAATGACTG GAGACAGCAT TCTATTTACA AGAACGGCTA CTGCCCAAAC
    CACCCCGTCA TTCAGTGGTT CTGGAAGGCT GTGCTACTCA TGGACGCCGA AAAGCGTATC
    CGGTTACTGC AGTTTGTCAC AGGGACATCG CGAGTACCTA TGAATGGATT TGCCGAACTT
    TATGGTTCCA ATGGTCCTCA GCTGTTTACA ATAGAGCAAT GGGGCAGTCC TGAGAAACTG
    CCCAGAGCTC ACACATGCTT TAATCGCCTT GACTTACCTC CATATGAAAC CTTTGAAGAT
    TTACGAGAGA AACTTCTCAT GGCCGTGGAA AATGCTCAAG GATTTGAAGG GGTGGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu65979
    Clone ID Related Accession (Same CDS sequence) XM_011525887.2 , XM_011525887.3
    Accession Version XM_011525887.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3036bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011525887.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAGACGTC TCGCATTTGA GCAGGGAGAG TCCCGTATTC TCAGAGTAAA AGTTGTTTCT 
    GGAATTGATC TCGCCAAAAA GGACATCTTT GGAGCCAGCT TCTGCTCCAT TGCTGTTGAA
    CTTTTTTTTG GTCGTGACAT CAGAAAAATA ACCAAGTGTG AGTCTGATAA TAGCAATGAT
    CCGTATGTGA AACTTTCATT GTACGTAGCG GATGAGAATA GAGAACTTGC TTTGGTCCAG
    ACAAAAACAA TTAAAAAGAC ACTGAACCCA AAATGGAATG AAGAATTTTA TTTCAGGGTA
    AACCCATCTA ATCACAGACT CCTATTTGAA GTATTTGACG AAAATAGACT GACACGAGAC
    GACTTCCTGG GCCAGGTGGA CGTGCCCCTT AGTCACCTTC CGACAGAAGA TCCAACCATG
    GAGCGACCCT ATACATTTAA GGACTTTCTC CTCAGACCAA GAAGTCATAA GTCTCGAGTT
    AAGGGATTTT TGCGATTGAA AATGGCCTAT ATGCCAAAAA ATGGAGGTCA AGATGAAGAA
    AACAGTGACC AGAGGGATGA CATGGAGCAT GGATGGGAAG TTGTTGACTC AAATGACTCG
    GCTTCTCAGC ACCAAGAGGA ACTTCCTCCT CCTCCTCTGC CTCCCGGGTG GGAAGAAAAA
    GTGGACAATT TAGGCCGAAC TTACTATGTC AACCACAACA ACCGGACCAC TCAGTGGCAC
    AGACCAAGCC TGATGGACGT GTCCTCGGAG TCGGACAATA ACATCAGACA GATCAACCAG
    GAGGCAGCAC ACCGGCGCTT CCGCTCCCGC AGGCACATCA GCGAAGACTT GGAGCCCGAG
    CCCTCGGAGG GCGGGGATGT CCCCGAGCCT TGGGAGACCA TTTCAGAGGA AGTGAATATC
    GCTGGAGACT CTCTCGGTCT GGCTCTGCCC CCACCACCGG CCTCCCCAGG ATCTCGGACC
    AGCCCTCAGG AGCTGTCAGA GGAACTAAGC AGAAGGCTTC AGATCACTCC AGACTCCAAT
    GGGGAACAGT TCAGCTCTTT GATTCAAAGA GAACCCTCCT CAAGGTTGAG GTCATGCAGT
    GTCACCGACG CAGTTGCAGA ACAGGGCCAT CTACCACCGC CCAGTGCCCC AGCTGGGAGA
    GCGCGTTCAT CAACTGTCAC GGGTGGTGAG GAACCAACGC CATCAGTGGC CTATGTACAT
    ACCACGCCGG GTCTGCCTTC AGGCTGGGAA GAAAGAAAAG ATGCTAAGGG GCGCACATAC
    TATGTCAATC ATAACAATCG AACCACAACT TGGACTCGAC CTATCATGCA GCTTGCAGAA
    GATGGTGCGT CCGGATCAGC CACAAACAGT AACAACCATC TAATCGAGCC TCAGATCCGC
    CGGCCTCGTA GCCTCAGCTC GCCAACAGTA ACTTTATCTG CCCCGCTGGA GGGTGCCAAG
    GACTCACCCG TACGTCGGGC TGTGAAAGAC ACCCTTTCCA ACCCACAGTC CCCACAGCCA
    TCACCTTACA ACTCCCCCAA ACCACAACAC AAAGTCACAC AGAGCTTCTT GCCACCCGGC
    TGGGAAATGA GGATAGCGCC AAACGGCCGG CCCTTCTTCA TTGATCATAA CACAAAGACT
    ACAACCTGGG AAGATCCACG TTTGAAATTT CCAGTACATA TGCGGTCAAA GACATCTTTA
    AACCCCAATG ACCTTGGCCC CCTTCCTCCT GGCTGGGAAG AAAGAATTCA CTTGGATGGC
    CGAACGTTTT ATATTGATCA TAGCAGCCAG GTGTTATGTG GAGAAAATGA TACCAGAGAA
    TCAGTGCCTT CATACGATAG CAAAATTACT CAGTGGGAAG ACCCAAGACT GCAGAACCCA
    GCTATTACTG GTCCGGCTGT CCCTTACTCC AGAGAATTTA AGCAGAAATA TGACTACTTC
    AGGAAGAAAT TAAAGAAACC TGCTGATATC CCCAATAGGT TTGAAATGAA ACTTCACAGA
    AATAACATAT TTGAAGAGTC CTATCGGAGA ATTATGTCCG TGAAAAGACC AGATGTCCTA
    AAAGCTAGAC TGTGGATTGA GTTTGAATCA GAGAAAGGTC TTGACTATGG GGGTGTGGCC
    AGAGAATGGT TCTTCTTACT GTCCAAAGAG ATGTTCAACC CCTACTACGG CCTCTTTGAG
    TACTCTGCCA CGGACAACTA CACCCTTCAG ATCAACCCTA ATTCAGGCCT CTGTAATGAG
    GATCATTTGT CCTACTTCAC TTTTATTGGA AGAGTTGCTG GTCTGGCCGT ATTTCATGGG
    AAGCTCTTAG ATGGTTTCTT CATTAGACCA TTTTACAAGA TGATGTTGGG AAAGCAGATA
    ACCCTGAATG ACATGGAATC TGTGGATAGT GAATATTACA ACTCTTTGAA ATGGATCCTG
    GAGAATGACC CTACTGAGCT GGACCTCATG TTCTGCATAG ACGAAGAAAA CTTTGGACAG
    ACATATCAAG TGGATTTGAA GCCCAATGGG TCAGAAATAA TGGTCACAAA TGAAAACAAA
    AGGGAATATA TCGACTTAGT CATCCAGTGG AGATTTGTGA ACAGGGTCCA GAAGCAGATG
    AACGCCTTCT TGGAGGGATT CACAGAACTA CTTCCTATTG ATTTGATTAA AATTTTTGAT
    GAAAATGAGC TGGAGTTGCT CATGTGCGGC CTCGGTGATG TGGATGTGAA TGACTGGAGA
    CAGCATTCTA TTTACAAGAA CGGCTACTGC CCAAACCACC CCGTCATTCA GTGGTTCTGG
    AAGGCTGTGC TACTCATGGA CGCCGAAAAG CGTATCCGGT TACTGCAGTT TGTCACAGGG
    ACATCGCGAG TACCTATGAA TGGATTTGCC GAACTTTATG GTTCCAATGG TCCTCAGCTG
    TTTACAATAG AGCAATGGGG CAGTCCTGAG AAACTGCCCA GAGCTCACAC ATGCTTTAAT
    CGCCTTGACT TACCTCCATA TGAAACCTTT GAAGATTTAC GAGAGAAACT TCTCATGGCC
    GTGGAAAATG CTCAAGGATT TGAAGGGGTG GATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011524189.1
    CDS304..3339
    Translation

    Target ORF information:

    RefSeq Version XM_011525887.2
    Organism Homo sapiens(human)
    Definition Homo sapiens neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011525887.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAGACGTC TCGCATTTGA GCAGGGAGAG TCCCGTATTC TCAGAGTAAA AGTTGTTTCT 
    GGAATTGATC TCGCCAAAAA GGACATCTTT GGAGCCAGCT TCTGCTCCAT TGCTGTTGAA
    CTTTTTTTTG GTCGTGACAT CAGAAAAATA ACCAAGTGTG AGTCTGATAA TAGCAATGAT
    CCGTATGTGA AACTTTCATT GTACGTAGCG GATGAGAATA GAGAACTTGC TTTGGTCCAG
    ACAAAAACAA TTAAAAAGAC ACTGAACCCA AAATGGAATG AAGAATTTTA TTTCAGGGTA
    AACCCATCTA ATCACAGACT CCTATTTGAA GTATTTGACG AAAATAGACT GACACGAGAC
    GACTTCCTGG GCCAGGTGGA CGTGCCCCTT AGTCACCTTC CGACAGAAGA TCCAACCATG
    GAGCGACCCT ATACATTTAA GGACTTTCTC CTCAGACCAA GAAGTCATAA GTCTCGAGTT
    AAGGGATTTT TGCGATTGAA AATGGCCTAT ATGCCAAAAA ATGGAGGTCA AGATGAAGAA
    AACAGTGACC AGAGGGATGA CATGGAGCAT GGATGGGAAG TTGTTGACTC AAATGACTCG
    GCTTCTCAGC ACCAAGAGGA ACTTCCTCCT CCTCCTCTGC CTCCCGGGTG GGAAGAAAAA
    GTGGACAATT TAGGCCGAAC TTACTATGTC AACCACAACA ACCGGACCAC TCAGTGGCAC
    AGACCAAGCC TGATGGACGT GTCCTCGGAG TCGGACAATA ACATCAGACA GATCAACCAG
    GAGGCAGCAC ACCGGCGCTT CCGCTCCCGC AGGCACATCA GCGAAGACTT GGAGCCCGAG
    CCCTCGGAGG GCGGGGATGT CCCCGAGCCT TGGGAGACCA TTTCAGAGGA AGTGAATATC
    GCTGGAGACT CTCTCGGTCT GGCTCTGCCC CCACCACCGG CCTCCCCAGG ATCTCGGACC
    AGCCCTCAGG AGCTGTCAGA GGAACTAAGC AGAAGGCTTC AGATCACTCC AGACTCCAAT
    GGGGAACAGT TCAGCTCTTT GATTCAAAGA GAACCCTCCT CAAGGTTGAG GTCATGCAGT
    GTCACCGACG CAGTTGCAGA ACAGGGCCAT CTACCACCGC CCAGTGCCCC AGCTGGGAGA
    GCGCGTTCAT CAACTGTCAC GGGTGGTGAG GAACCAACGC CATCAGTGGC CTATGTACAT
    ACCACGCCGG GTCTGCCTTC AGGCTGGGAA GAAAGAAAAG ATGCTAAGGG GCGCACATAC
    TATGTCAATC ATAACAATCG AACCACAACT TGGACTCGAC CTATCATGCA GCTTGCAGAA
    GATGGTGCGT CCGGATCAGC CACAAACAGT AACAACCATC TAATCGAGCC TCAGATCCGC
    CGGCCTCGTA GCCTCAGCTC GCCAACAGTA ACTTTATCTG CCCCGCTGGA GGGTGCCAAG
    GACTCACCCG TACGTCGGGC TGTGAAAGAC ACCCTTTCCA ACCCACAGTC CCCACAGCCA
    TCACCTTACA ACTCCCCCAA ACCACAACAC AAAGTCACAC AGAGCTTCTT GCCACCCGGC
    TGGGAAATGA GGATAGCGCC AAACGGCCGG CCCTTCTTCA TTGATCATAA CACAAAGACT
    ACAACCTGGG AAGATCCACG TTTGAAATTT CCAGTACATA TGCGGTCAAA GACATCTTTA
    AACCCCAATG ACCTTGGCCC CCTTCCTCCT GGCTGGGAAG AAAGAATTCA CTTGGATGGC
    CGAACGTTTT ATATTGATCA TAGCAGCCAG GTGTTATGTG GAGAAAATGA TACCAGAGAA
    TCAGTGCCTT CATACGATAG CAAAATTACT CAGTGGGAAG ACCCAAGACT GCAGAACCCA
    GCTATTACTG GTCCGGCTGT CCCTTACTCC AGAGAATTTA AGCAGAAATA TGACTACTTC
    AGGAAGAAAT TAAAGAAACC TGCTGATATC CCCAATAGGT TTGAAATGAA ACTTCACAGA
    AATAACATAT TTGAAGAGTC CTATCGGAGA ATTATGTCCG TGAAAAGACC AGATGTCCTA
    AAAGCTAGAC TGTGGATTGA GTTTGAATCA GAGAAAGGTC TTGACTATGG GGGTGTGGCC
    AGAGAATGGT TCTTCTTACT GTCCAAAGAG ATGTTCAACC CCTACTACGG CCTCTTTGAG
    TACTCTGCCA CGGACAACTA CACCCTTCAG ATCAACCCTA ATTCAGGCCT CTGTAATGAG
    GATCATTTGT CCTACTTCAC TTTTATTGGA AGAGTTGCTG GTCTGGCCGT ATTTCATGGG
    AAGCTCTTAG ATGGTTTCTT CATTAGACCA TTTTACAAGA TGATGTTGGG AAAGCAGATA
    ACCCTGAATG ACATGGAATC TGTGGATAGT GAATATTACA ACTCTTTGAA ATGGATCCTG
    GAGAATGACC CTACTGAGCT GGACCTCATG TTCTGCATAG ACGAAGAAAA CTTTGGACAG
    ACATATCAAG TGGATTTGAA GCCCAATGGG TCAGAAATAA TGGTCACAAA TGAAAACAAA
    AGGGAATATA TCGACTTAGT CATCCAGTGG AGATTTGTGA ACAGGGTCCA GAAGCAGATG
    AACGCCTTCT TGGAGGGATT CACAGAACTA CTTCCTATTG ATTTGATTAA AATTTTTGAT
    GAAAATGAGC TGGAGTTGCT CATGTGCGGC CTCGGTGATG TGGATGTGAA TGACTGGAGA
    CAGCATTCTA TTTACAAGAA CGGCTACTGC CCAAACCACC CCGTCATTCA GTGGTTCTGG
    AAGGCTGTGC TACTCATGGA CGCCGAAAAG CGTATCCGGT TACTGCAGTT TGTCACAGGG
    ACATCGCGAG TACCTATGAA TGGATTTGCC GAACTTTATG GTTCCAATGG TCCTCAGCTG
    TTTACAATAG AGCAATGGGG CAGTCCTGAG AAACTGCCCA GAGCTCACAC ATGCTTTAAT
    CGCCTTGACT TACCTCCATA TGAAACCTTT GAAGATTTAC GAGAGAAACT TCTCATGGCC
    GTGGAAAATG CTCAAGGATT TGAAGGGGTG GATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu65979
    Clone ID Related Accession (Same CDS sequence) XM_011525887.2 , XM_011525887.3
    Accession Version XM_011525887.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3036bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011525887.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAGACGTC TCGCATTTGA GCAGGGAGAG TCCCGTATTC TCAGAGTAAA AGTTGTTTCT 
    GGAATTGATC TCGCCAAAAA GGACATCTTT GGAGCCAGCT TCTGCTCCAT TGCTGTTGAA
    CTTTTTTTTG GTCGTGACAT CAGAAAAATA ACCAAGTGTG AGTCTGATAA TAGCAATGAT
    CCGTATGTGA AACTTTCATT GTACGTAGCG GATGAGAATA GAGAACTTGC TTTGGTCCAG
    ACAAAAACAA TTAAAAAGAC ACTGAACCCA AAATGGAATG AAGAATTTTA TTTCAGGGTA
    AACCCATCTA ATCACAGACT CCTATTTGAA GTATTTGACG AAAATAGACT GACACGAGAC
    GACTTCCTGG GCCAGGTGGA CGTGCCCCTT AGTCACCTTC CGACAGAAGA TCCAACCATG
    GAGCGACCCT ATACATTTAA GGACTTTCTC CTCAGACCAA GAAGTCATAA GTCTCGAGTT
    AAGGGATTTT TGCGATTGAA AATGGCCTAT ATGCCAAAAA ATGGAGGTCA AGATGAAGAA
    AACAGTGACC AGAGGGATGA CATGGAGCAT GGATGGGAAG TTGTTGACTC AAATGACTCG
    GCTTCTCAGC ACCAAGAGGA ACTTCCTCCT CCTCCTCTGC CTCCCGGGTG GGAAGAAAAA
    GTGGACAATT TAGGCCGAAC TTACTATGTC AACCACAACA ACCGGACCAC TCAGTGGCAC
    AGACCAAGCC TGATGGACGT GTCCTCGGAG TCGGACAATA ACATCAGACA GATCAACCAG
    GAGGCAGCAC ACCGGCGCTT CCGCTCCCGC AGGCACATCA GCGAAGACTT GGAGCCCGAG
    CCCTCGGAGG GCGGGGATGT CCCCGAGCCT TGGGAGACCA TTTCAGAGGA AGTGAATATC
    GCTGGAGACT CTCTCGGTCT GGCTCTGCCC CCACCACCGG CCTCCCCAGG ATCTCGGACC
    AGCCCTCAGG AGCTGTCAGA GGAACTAAGC AGAAGGCTTC AGATCACTCC AGACTCCAAT
    GGGGAACAGT TCAGCTCTTT GATTCAAAGA GAACCCTCCT CAAGGTTGAG GTCATGCAGT
    GTCACCGACG CAGTTGCAGA ACAGGGCCAT CTACCACCGC CCAGTGCCCC AGCTGGGAGA
    GCGCGTTCAT CAACTGTCAC GGGTGGTGAG GAACCAACGC CATCAGTGGC CTATGTACAT
    ACCACGCCGG GTCTGCCTTC AGGCTGGGAA GAAAGAAAAG ATGCTAAGGG GCGCACATAC
    TATGTCAATC ATAACAATCG AACCACAACT TGGACTCGAC CTATCATGCA GCTTGCAGAA
    GATGGTGCGT CCGGATCAGC CACAAACAGT AACAACCATC TAATCGAGCC TCAGATCCGC
    CGGCCTCGTA GCCTCAGCTC GCCAACAGTA ACTTTATCTG CCCCGCTGGA GGGTGCCAAG
    GACTCACCCG TACGTCGGGC TGTGAAAGAC ACCCTTTCCA ACCCACAGTC CCCACAGCCA
    TCACCTTACA ACTCCCCCAA ACCACAACAC AAAGTCACAC AGAGCTTCTT GCCACCCGGC
    TGGGAAATGA GGATAGCGCC AAACGGCCGG CCCTTCTTCA TTGATCATAA CACAAAGACT
    ACAACCTGGG AAGATCCACG TTTGAAATTT CCAGTACATA TGCGGTCAAA GACATCTTTA
    AACCCCAATG ACCTTGGCCC CCTTCCTCCT GGCTGGGAAG AAAGAATTCA CTTGGATGGC
    CGAACGTTTT ATATTGATCA TAGCAGCCAG GTGTTATGTG GAGAAAATGA TACCAGAGAA
    TCAGTGCCTT CATACGATAG CAAAATTACT CAGTGGGAAG ACCCAAGACT GCAGAACCCA
    GCTATTACTG GTCCGGCTGT CCCTTACTCC AGAGAATTTA AGCAGAAATA TGACTACTTC
    AGGAAGAAAT TAAAGAAACC TGCTGATATC CCCAATAGGT TTGAAATGAA ACTTCACAGA
    AATAACATAT TTGAAGAGTC CTATCGGAGA ATTATGTCCG TGAAAAGACC AGATGTCCTA
    AAAGCTAGAC TGTGGATTGA GTTTGAATCA GAGAAAGGTC TTGACTATGG GGGTGTGGCC
    AGAGAATGGT TCTTCTTACT GTCCAAAGAG ATGTTCAACC CCTACTACGG CCTCTTTGAG
    TACTCTGCCA CGGACAACTA CACCCTTCAG ATCAACCCTA ATTCAGGCCT CTGTAATGAG
    GATCATTTGT CCTACTTCAC TTTTATTGGA AGAGTTGCTG GTCTGGCCGT ATTTCATGGG
    AAGCTCTTAG ATGGTTTCTT CATTAGACCA TTTTACAAGA TGATGTTGGG AAAGCAGATA
    ACCCTGAATG ACATGGAATC TGTGGATAGT GAATATTACA ACTCTTTGAA ATGGATCCTG
    GAGAATGACC CTACTGAGCT GGACCTCATG TTCTGCATAG ACGAAGAAAA CTTTGGACAG
    ACATATCAAG TGGATTTGAA GCCCAATGGG TCAGAAATAA TGGTCACAAA TGAAAACAAA
    AGGGAATATA TCGACTTAGT CATCCAGTGG AGATTTGTGA ACAGGGTCCA GAAGCAGATG
    AACGCCTTCT TGGAGGGATT CACAGAACTA CTTCCTATTG ATTTGATTAA AATTTTTGAT
    GAAAATGAGC TGGAGTTGCT CATGTGCGGC CTCGGTGATG TGGATGTGAA TGACTGGAGA
    CAGCATTCTA TTTACAAGAA CGGCTACTGC CCAAACCACC CCGTCATTCA GTGGTTCTGG
    AAGGCTGTGC TACTCATGGA CGCCGAAAAG CGTATCCGGT TACTGCAGTT TGTCACAGGG
    ACATCGCGAG TACCTATGAA TGGATTTGCC GAACTTTATG GTTCCAATGG TCCTCAGCTG
    TTTACAATAG AGCAATGGGG CAGTCCTGAG AAACTGCCCA GAGCTCACAC ATGCTTTAAT
    CGCCTTGACT TACCTCCATA TGAAACCTTT GAAGATTTAC GAGAGAAACT TCTCATGGCC
    GTGGAAAATG CTCAAGGATT TGAAGGGGTG GATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011524189.1
    CDS305..3340
    Translation

    Target ORF information:

    RefSeq Version XM_011525887.3
    Organism Homo sapiens(human)
    Definition Homo sapiens NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011525887.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAGACGTC TCGCATTTGA GCAGGGAGAG TCCCGTATTC TCAGAGTAAA AGTTGTTTCT 
    GGAATTGATC TCGCCAAAAA GGACATCTTT GGAGCCAGCT TCTGCTCCAT TGCTGTTGAA
    CTTTTTTTTG GTCGTGACAT CAGAAAAATA ACCAAGTGTG AGTCTGATAA TAGCAATGAT
    CCGTATGTGA AACTTTCATT GTACGTAGCG GATGAGAATA GAGAACTTGC TTTGGTCCAG
    ACAAAAACAA TTAAAAAGAC ACTGAACCCA AAATGGAATG AAGAATTTTA TTTCAGGGTA
    AACCCATCTA ATCACAGACT CCTATTTGAA GTATTTGACG AAAATAGACT GACACGAGAC
    GACTTCCTGG GCCAGGTGGA CGTGCCCCTT AGTCACCTTC CGACAGAAGA TCCAACCATG
    GAGCGACCCT ATACATTTAA GGACTTTCTC CTCAGACCAA GAAGTCATAA GTCTCGAGTT
    AAGGGATTTT TGCGATTGAA AATGGCCTAT ATGCCAAAAA ATGGAGGTCA AGATGAAGAA
    AACAGTGACC AGAGGGATGA CATGGAGCAT GGATGGGAAG TTGTTGACTC AAATGACTCG
    GCTTCTCAGC ACCAAGAGGA ACTTCCTCCT CCTCCTCTGC CTCCCGGGTG GGAAGAAAAA
    GTGGACAATT TAGGCCGAAC TTACTATGTC AACCACAACA ACCGGACCAC TCAGTGGCAC
    AGACCAAGCC TGATGGACGT GTCCTCGGAG TCGGACAATA ACATCAGACA GATCAACCAG
    GAGGCAGCAC ACCGGCGCTT CCGCTCCCGC AGGCACATCA GCGAAGACTT GGAGCCCGAG
    CCCTCGGAGG GCGGGGATGT CCCCGAGCCT TGGGAGACCA TTTCAGAGGA AGTGAATATC
    GCTGGAGACT CTCTCGGTCT GGCTCTGCCC CCACCACCGG CCTCCCCAGG ATCTCGGACC
    AGCCCTCAGG AGCTGTCAGA GGAACTAAGC AGAAGGCTTC AGATCACTCC AGACTCCAAT
    GGGGAACAGT TCAGCTCTTT GATTCAAAGA GAACCCTCCT CAAGGTTGAG GTCATGCAGT
    GTCACCGACG CAGTTGCAGA ACAGGGCCAT CTACCACCGC CCAGTGCCCC AGCTGGGAGA
    GCGCGTTCAT CAACTGTCAC GGGTGGTGAG GAACCAACGC CATCAGTGGC CTATGTACAT
    ACCACGCCGG GTCTGCCTTC AGGCTGGGAA GAAAGAAAAG ATGCTAAGGG GCGCACATAC
    TATGTCAATC ATAACAATCG AACCACAACT TGGACTCGAC CTATCATGCA GCTTGCAGAA
    GATGGTGCGT CCGGATCAGC CACAAACAGT AACAACCATC TAATCGAGCC TCAGATCCGC
    CGGCCTCGTA GCCTCAGCTC GCCAACAGTA ACTTTATCTG CCCCGCTGGA GGGTGCCAAG
    GACTCACCCG TACGTCGGGC TGTGAAAGAC ACCCTTTCCA ACCCACAGTC CCCACAGCCA
    TCACCTTACA ACTCCCCCAA ACCACAACAC AAAGTCACAC AGAGCTTCTT GCCACCCGGC
    TGGGAAATGA GGATAGCGCC AAACGGCCGG CCCTTCTTCA TTGATCATAA CACAAAGACT
    ACAACCTGGG AAGATCCACG TTTGAAATTT CCAGTACATA TGCGGTCAAA GACATCTTTA
    AACCCCAATG ACCTTGGCCC CCTTCCTCCT GGCTGGGAAG AAAGAATTCA CTTGGATGGC
    CGAACGTTTT ATATTGATCA TAGCAGCCAG GTGTTATGTG GAGAAAATGA TACCAGAGAA
    TCAGTGCCTT CATACGATAG CAAAATTACT CAGTGGGAAG ACCCAAGACT GCAGAACCCA
    GCTATTACTG GTCCGGCTGT CCCTTACTCC AGAGAATTTA AGCAGAAATA TGACTACTTC
    AGGAAGAAAT TAAAGAAACC TGCTGATATC CCCAATAGGT TTGAAATGAA ACTTCACAGA
    AATAACATAT TTGAAGAGTC CTATCGGAGA ATTATGTCCG TGAAAAGACC AGATGTCCTA
    AAAGCTAGAC TGTGGATTGA GTTTGAATCA GAGAAAGGTC TTGACTATGG GGGTGTGGCC
    AGAGAATGGT TCTTCTTACT GTCCAAAGAG ATGTTCAACC CCTACTACGG CCTCTTTGAG
    TACTCTGCCA CGGACAACTA CACCCTTCAG ATCAACCCTA ATTCAGGCCT CTGTAATGAG
    GATCATTTGT CCTACTTCAC TTTTATTGGA AGAGTTGCTG GTCTGGCCGT ATTTCATGGG
    AAGCTCTTAG ATGGTTTCTT CATTAGACCA TTTTACAAGA TGATGTTGGG AAAGCAGATA
    ACCCTGAATG ACATGGAATC TGTGGATAGT GAATATTACA ACTCTTTGAA ATGGATCCTG
    GAGAATGACC CTACTGAGCT GGACCTCATG TTCTGCATAG ACGAAGAAAA CTTTGGACAG
    ACATATCAAG TGGATTTGAA GCCCAATGGG TCAGAAATAA TGGTCACAAA TGAAAACAAA
    AGGGAATATA TCGACTTAGT CATCCAGTGG AGATTTGTGA ACAGGGTCCA GAAGCAGATG
    AACGCCTTCT TGGAGGGATT CACAGAACTA CTTCCTATTG ATTTGATTAA AATTTTTGAT
    GAAAATGAGC TGGAGTTGCT CATGTGCGGC CTCGGTGATG TGGATGTGAA TGACTGGAGA
    CAGCATTCTA TTTACAAGAA CGGCTACTGC CCAAACCACC CCGTCATTCA GTGGTTCTGG
    AAGGCTGTGC TACTCATGGA CGCCGAAAAG CGTATCCGGT TACTGCAGTT TGTCACAGGG
    ACATCGCGAG TACCTATGAA TGGATTTGCC GAACTTTATG GTTCCAATGG TCCTCAGCTG
    TTTACAATAG AGCAATGGGG CAGTCCTGAG AAACTGCCCA GAGCTCACAC ATGCTTTAAT
    CGCCTTGACT TACCTCCATA TGAAACCTTT GAAGATTTAC GAGAGAAACT TCTCATGGCC
    GTGGAAAATG CTCAAGGATT TGAAGGGGTG GATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.