RSP5 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	RSP5
Entrez Gene ID	856862
Full Name	NEDD4 family E3 ubiquitin-protein ligase
Synonyms	MDP1,MUT2,NPI1,SMM1,UBY1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179015.3	NP_011051.3	NEDD4 family E3 ubiquitin-protein ligase

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	NEDD4L	XP_001140979.1
Canis lupus familiaris (dog)	NEDD4L	XP_005615425.1
Bos taurus (cattle)	NEDD4L	XP_005224408.1
Schizosaccharomyces pombe (fission yeast)	pub1	NP_594396.1
	NEDD4L	XP_001088661.2
Gallus gallus (chicken)	NEDD4L	XP_004949055.1
Eremothecium gossypii	AGOS_ADL055C	NP_984041.1
Neurospora crassa	NCU03947	XP_957586.2
Homo sapiens (human)	NEDD4L	NP_001138439.1
Drosophila melanogaster (fruit fly)	Nedd4	NP_730284.2
Xenopus tropicalis (tropical clawed frog)	nedd4l	NP_001006727.1
Caenorhabditis elegans (roundworm)	Y92H12A.2	NP_490865.3
Magnaporthe oryzae (rice blast fungus)	MGG_07255	XP_003715474.1
Mus musculus (house mouse)	Nedd4l	NP_001107858.1
Rattus norvegicus (Norway rat)	Nedd4l	NP_001008301.2
Danio rerio (zebrafish)	nedd4l	XP_005161145.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000787	XP_311327.5
Saccharomyces cerevisiae (baker's yeast)	RSP5	NP_011051.3
Kluyveromyces lactis	KLLA0E13575g	XP_454562.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome V.
Dietrich FS, Mulligan J, Hennessy K, Yelton MA, Allen E, Araujo R, Aviles E, Berno A, Brennan T, Carpenter J, Chen E, Cherry JM, Chung E, Duncan M, Guzman E, Hartzell G, Hunicke-Smith S, Hyman RW, Kayser A, Komp C, Lashkari D, Lew H, Lin D, Mosedale D, Davis RW
Nature387(6632 Suppl)78-81(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Mdm34 and Mdm12, 2 components of the endoplasmic reticulum-mitochondrial encounter structure, are ubiquitinated by the E3 ligase Rsp5
Title: Ubiquitination of ERMES components by the E3 ligase Rsp5 is involved in mitophagy.

Authors present the molecular features in the methionine permease Mup1 that are required for Art1-Rsp5-mediated ubiquitination and endocytosis.
Title: Identification of the endocytic sorting signal recognized by the Art1-Rsp5 ubiquitin ligase complex.

Rsp5 may be phosphorylated by PKA at position T761 and that this regulation is important for its localization and function.
Title: Mimicking the phosphorylation of Rsp5 in PKA site T761 affects its function and cellular localization.

While rsp5(T255A), RSP5(T357A), and rsp5(T413A) impaired the interaction of Rsp5 with Bul1, the RSP5(T357A) mutation specifically augmented the interaction with Bul2.
Title: Cooperative and selective roles of the WW domains of the yeast Nedd4-like ubiquitin ligase Rsp5 in the recognition of the arrestin-like adaptors Bul1 and Bul2.

Hem12 is monoubiquitinated in vivo by Rsp5
Title: Hem12, an enzyme of heme biosynthesis pathway, is monoubiquitinated by Rsp5 ubiquitin ligase in yeast cells.

The following RSP5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RSP5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi05620	NM_001179015.3 Latest version!	Saccharomyces cerevisiae S288C NEDD4 family E3 ubiquitin-protein ligase (RSP5), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RSP5 ( NM_001179015.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_011051.3 Saccharomyces cerevisiae S288C NEDD4 family E3 ubiquitin-protein ligase (RSP5), partial mRNA.

Price & Availability↑

CloneID	OSi05620
Clone ID Related Accession (Same CDS sequence)	NM_001179015.3
Accession Version	NM_001179015.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2430bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	NEDD4 family E3 ubiquitin-protein ligase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001137). On Jul 30, 2012 this sequence version replaced NM_001179015.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGCCTTCAT CCATATCCGT CAAGTTAGTG GCTGCAGAGT CATTATATAA GAGGGACGTA 
TTCCGTTCCC CGGATCCGTT TGCTGTTCTG ACTATTGATG GTTACCAAAC CAAATCTACA 
TCCGCAGCGA AGAAAACGTT AAATCCCTAC TGGAATGAGA CTTTCAAATT CGACGATATC 
AATGAAAATT CTATTTTAAC CATCCAAGTC TTTGATCAAA AGAAATTTAA AAAGAAGGAT 
CAAGGGTTTC TTGGCGTGGT TAACGTCCGC GTGGGTGATG TTTTGGGCCA TTTGGATGAA 
GATACTGCTA CATCGAGTGG TAGACCTCGC GAAGAAACTA TTACTCGTGA TTTAAAAAAA 
TCTAATGACG GAATGGCCGT CAGCGGTAGG TTGATTGTTG TTTTATCAAA ACTACCTTCG 
TCAAGTCCGC ATTCACAAGC TCCTTCTGGC CATACTGCAT CTTCTAGTAC GAATACAAGT 
TCAACTACTC GAACAAATGG TCATTCTACT TCCTCCACCC GTAATCATTC AACGTCTCAT 
CCTTCCAGAG GCACCGCGCA AGCAGTAGAA TCAACTCTTC AAAGCGGTAC AACAGCTGCT 
ACTAATACGG CAACAACCAG TCACCGTTCC ACCAATTCCA CATCCAGTGC TACCAGACAA 
TACTCTTCGT TTGAAGACCA GTATGGTCGT TTACCCCCTG GTTGGGAGAG AAGGACCGAT 
AACTTTGGTC GTACATATTA CGTCGATCAT AACACAAGGA CTACCACTTG GAAACGTCCA 
ACGCTCGATC AAACAGAAGC CGAACGTGGC AACCAATTAA ATGCAAATAC TGAGTTAGAG 
AGAAGGCAGC ATAGGGGAAG AACTTTACCT GGTGGATCAT CAGATAATTC CTCTGTAACA 
GTTCAAGTGG GAGGTGGTTC CAATATACCT CCTGTTAATG GTGCAGCAGC AGCAGCGTTT 
GCAGCTACAG GTGGTACCAC ATCTGGTTTA GGCGAATTAC CTTCAGGCTG GGAGCAGCGA 
TTTACTCCAG AAGGAAGAGC TTATTTCGTT GACCATAATA CTAGAACAAC CACTTGGGTG 
GATCCAAGGA GACAACAGTA TATACGTACT TATGGCCCTA CAAATACCAC CATTCAGCAA 
CAACCGGTCT CCCAATTAGG TCCTTTGCCT TCTGGATGGG AAATGAGATT GACCAATACG 
GCACGTGTAT ATTTCGTTGA CCACAATACA AAAACAACGA CCTGGGATGA CCCAAGACTT 
CCATCATCGC TAGACCAAAA TGTTCCACAA TACAAGCGTG ACTTCAGACG TAAGGTTATT 
TATTTCAGGT CCCAGCCCGC TCTTAGAATA TTGCCGGGAC AATGTCATAT TAAAGTACGT 
AGAAAGAACA TTTTTGAGGA TGCTTATCAA GAAATTATGA GACAAACCCC AGAAGATTTA 
AAGAAAAGAT TAATGATCAA GTTTGATGGT GAGGAAGGTT TAGATTACGG TGGTGTTTCC 
AGAGAATTTT TCTTTTTATT ATCACATGAG ATGTTTAATC CATTTTATTG TTTGTTTGAA 
TATTCTGCGT ATGACAACTA TACCATTCAA ATAAATCCTA ACAGTGGCAT CAACCCCGAA 
CATTTGAACT ATTTCAAATT CATTGGTAGA GTTGTGGGTC TTGGTGTTTT CCATAGAAGA 
TTTTTGGATG CATTCTTTGT AGGTGCACTT TACAAAATGA TGCTACGTAA AAAAGTCGTA 
TTGCAAGATA TGGAAGGTGT TGACGCAGAG GTGTATAACT CATTAAACTG GATGCTTGAG 
AATAGTATAG ATGGTGTCTT GGATCTGACG TTCAGTGCCG ACGATGAAAG ATTCGGTGAA 
GTTGTAACAG TGGATTTGAA GCCAGATGGG AGAAATATTG AAGTTACTGA TGGTAATAAG 
AAAGAATATG TCGAATTATA TACCCAATGG AGAATTGTTG ATAGAGTTCA AGAACAATTT 
AAGGCATTCA TGGATGGTTT TAACGAATTA ATCCCCGAAG ACTTAGTAAC TGTGTTTGAT 
GAGCGTGAAC TAGAACTATT GATCGGTGGT ATTGCGGAAA TTGACATTGA AGATTGGAAG 
AAACACACTG ATTATCGTGG TTACCAAGAG TCAGACGAGG TCATTCAATG GTTTTGGAAG 
TGTGTCAGTG AATGGGATAA TGAACAAAGA GCCCGTTTAT TGCAGTTCAC CACTGGTACT 
TCTCGTATAC CTGTCAACGG GTTTAAAGAT TTGCAAGGCT CTGATGGTCC AAGAAGATTC 
ACTATTGAAA AAGCTGGTGA AGTACAACAA TTGCCAAAAT CTCACACATG TTTTAACAGA 
GTTGATTTGC CACAATACGT TGATTATGAC AGCATGAAAC AGAAGCTAAC ATTGGCCGTG 
GAAGAAACCA TAGGGTTTGG TCAAGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_011051.3
CDS	1..2430
Translation	MPSSISVKLVAAESLYKRDVFRSPDPFAVLTIDGYQTKSTSAAKKTLNPYWNETFKFDDINENSILTIQVFDQKKFKKKDQGFLGVVNVRVGDVLGHLDEDTATSSGRPREETITRDLKKSNDGMAVSGRLIVVLSKLPSSSPHSQAPSGHTASSSTNTSSTTRTNGHSTSSTRNHSTSHPSRGTAQAVESTLQSGTTAATNTATTSHRSTNSTSSATRQYSSFEDQYGRLPPGWERRTDNFGRTYYVDHNTRTTTWKRPTLDQTEAERGNQLNANTELERRQHRGRTLPGGSSDNSSVTVQVGGGSNIPPVNGAAAAAFAATGGTTSGLGELPSGWEQRFTPEGRAYFVDHNTRTTTWVDPRRQQYIRTYGPTNTTIQQQPVSQLGPLPSGWEMRLTNTARVYFVDHNTKTTTWDDPRLPSSLDQNVPQYKRDFRRKVIYFRSQPALRILPGQCHIKVRRKNIFEDAYQEIMRQTPEDLKKRLMIKFDGEEGLDYGGVSREFFFLLSHEMFNPFYCLFEYSAYDNYTIQINPNSGINPEHLNYFKFIGRVVGLGVFHRRFLDAFFVGALYKMMLRKKVVLQDMEGVDAEVYNSLNWMLENSIDGVLDLTFSADDERFGEVVTVDLKPDGRNIEVTDGNKKEYVELYTQWRIVDRVQEQFKAFMDGFNELIPEDLVTVFDERELELLIGGIAEIDIEDWKKHTDYRGYQESDEVIQWFWKCVSEWDNEQRARLLQFTTGTSRIPVNGFKDLQGSDGPRRFTIEKAGEVQQLPKSHTCFNRVDLPQYVDYDSMKQKLTLAVEETIGFGQE

Target ORF information:

RefSeq Version	NM_001179015.3
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C NEDD4 family E3 ubiquitin-protein ligase (RSP5), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179015.3

ORF Insert Sequence:

ATGCCTTCAT CCATATCCGT CAAGTTAGTG GCTGCAGAGT CATTATATAA GAGGGACGTA 
TTCCGTTCCC CGGATCCGTT TGCTGTTCTG ACTATTGATG GTTACCAAAC CAAATCTACA 
TCCGCAGCGA AGAAAACGTT AAATCCCTAC TGGAATGAGA CTTTCAAATT CGACGATATC 
AATGAAAATT CTATTTTAAC CATCCAAGTC TTTGATCAAA AGAAATTTAA AAAGAAGGAT 
CAAGGGTTTC TTGGCGTGGT TAACGTCCGC GTGGGTGATG TTTTGGGCCA TTTGGATGAA 
GATACTGCTA CATCGAGTGG TAGACCTCGC GAAGAAACTA TTACTCGTGA TTTAAAAAAA 
TCTAATGACG GAATGGCCGT CAGCGGTAGG TTGATTGTTG TTTTATCAAA ACTACCTTCG 
TCAAGTCCGC ATTCACAAGC TCCTTCTGGC CATACTGCAT CTTCTAGTAC GAATACAAGT 
TCAACTACTC GAACAAATGG TCATTCTACT TCCTCCACCC GTAATCATTC AACGTCTCAT 
CCTTCCAGAG GCACCGCGCA AGCAGTAGAA TCAACTCTTC AAAGCGGTAC AACAGCTGCT 
ACTAATACGG CAACAACCAG TCACCGTTCC ACCAATTCCA CATCCAGTGC TACCAGACAA 
TACTCTTCGT TTGAAGACCA GTATGGTCGT TTACCCCCTG GTTGGGAGAG AAGGACCGAT 
AACTTTGGTC GTACATATTA CGTCGATCAT AACACAAGGA CTACCACTTG GAAACGTCCA 
ACGCTCGATC AAACAGAAGC CGAACGTGGC AACCAATTAA ATGCAAATAC TGAGTTAGAG 
AGAAGGCAGC ATAGGGGAAG AACTTTACCT GGTGGATCAT CAGATAATTC CTCTGTAACA 
GTTCAAGTGG GAGGTGGTTC CAATATACCT CCTGTTAATG GTGCAGCAGC AGCAGCGTTT 
GCAGCTACAG GTGGTACCAC ATCTGGTTTA GGCGAATTAC CTTCAGGCTG GGAGCAGCGA 
TTTACTCCAG AAGGAAGAGC TTATTTCGTT GACCATAATA CTAGAACAAC CACTTGGGTG 
GATCCAAGGA GACAACAGTA TATACGTACT TATGGCCCTA CAAATACCAC CATTCAGCAA 
CAACCGGTCT CCCAATTAGG TCCTTTGCCT TCTGGATGGG AAATGAGATT GACCAATACG 
GCACGTGTAT ATTTCGTTGA CCACAATACA AAAACAACGA CCTGGGATGA CCCAAGACTT 
CCATCATCGC TAGACCAAAA TGTTCCACAA TACAAGCGTG ACTTCAGACG TAAGGTTATT 
TATTTCAGGT CCCAGCCCGC TCTTAGAATA TTGCCGGGAC AATGTCATAT TAAAGTACGT 
AGAAAGAACA TTTTTGAGGA TGCTTATCAA GAAATTATGA GACAAACCCC AGAAGATTTA 
AAGAAAAGAT TAATGATCAA GTTTGATGGT GAGGAAGGTT TAGATTACGG TGGTGTTTCC 
AGAGAATTTT TCTTTTTATT ATCACATGAG ATGTTTAATC CATTTTATTG TTTGTTTGAA 
TATTCTGCGT ATGACAACTA TACCATTCAA ATAAATCCTA ACAGTGGCAT CAACCCCGAA 
CATTTGAACT ATTTCAAATT CATTGGTAGA GTTGTGGGTC TTGGTGTTTT CCATAGAAGA 
TTTTTGGATG CATTCTTTGT AGGTGCACTT TACAAAATGA TGCTACGTAA AAAAGTCGTA 
TTGCAAGATA TGGAAGGTGT TGACGCAGAG GTGTATAACT CATTAAACTG GATGCTTGAG 
AATAGTATAG ATGGTGTCTT GGATCTGACG TTCAGTGCCG ACGATGAAAG ATTCGGTGAA 
GTTGTAACAG TGGATTTGAA GCCAGATGGG AGAAATATTG AAGTTACTGA TGGTAATAAG 
AAAGAATATG TCGAATTATA TACCCAATGG AGAATTGTTG ATAGAGTTCA AGAACAATTT 
AAGGCATTCA TGGATGGTTT TAACGAATTA ATCCCCGAAG ACTTAGTAAC TGTGTTTGAT 
GAGCGTGAAC TAGAACTATT GATCGGTGGT ATTGCGGAAA TTGACATTGA AGATTGGAAG 
AAACACACTG ATTATCGTGG TTACCAAGAG TCAGACGAGG TCATTCAATG GTTTTGGAAG 
TGTGTCAGTG AATGGGATAA TGAACAAAGA GCCCGTTTAT TGCAGTTCAC CACTGGTACT 
TCTCGTATAC CTGTCAACGG GTTTAAAGAT TTGCAAGGCT CTGATGGTCC AAGAAGATTC 
ACTATTGAAA AAGCTGGTGA AGTACAACAA TTGCCAAAAT CTCACACATG TTTTAACAGA 
GTTGATTTGC CACAATACGT TGATTATGAC AGCATGAAAC AGAAGCTAAC ATTGGCCGTG 
GAAGAAACCA TAGGGTTTGG TCAAGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome V.
Nature387(6632 Suppl)78-81(1997 May)
Dietrich FS,Mulligan J,Hennessy K,Yelton MA,Allen E,Araujo R,Aviles E,Berno A,Brennan T,Carpenter J,Chen E,Cherry JM,Chung E,Duncan M,Guzman E,Hartzell G,Hunicke-Smith S,Hyman RW,Kayser A,Komp C,Lashkari D,Lew H,Lin D,Mosedale D,Davis RW

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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RSP5 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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