dapk1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	dapk1
Entrez Gene ID	558314
Full Name	death-associated protein kinase 1
Synonyms	si:ch211-66i11.1,wu:fj09c03
General protein information	Preferred Names death-associated protein kinase 1 Names death-associated protein kinase 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001099990.2	NP_001093460.1	death-associated protein kinase 1
XM_017356101.1	XP_017211590.1	death-associated protein kinase 1 isoform X1
XM_017356102.1	XP_017211591.1	death-associated protein kinase 1 isoform X2
XM_017356100.2	XP_017211589.1	death-associated protein kinase 1 isoform X1
XM_017356100.1	XP_017211589.1	death-associated protein kinase 1 isoform X1
XM_017356101.2	XP_017211590.1	death-associated protein kinase 1 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	DAPK1	NP_004929.2
Xenopus tropicalis (tropical clawed frog)	dapk1	NP_001116911.1
Rattus norvegicus (Norway rat)	Dapk1	NP_001100805.2
Danio rerio (zebrafish)	dapk1	NP_001093460.1
Pan troglodytes (chimpanzee)	DAPK1	XP_001140200.1
	DAPK1	XP_001084752.1
Bos taurus (cattle)	DAPK1	NP_001192967.1
Gallus gallus (chicken)	DAPK1	NP_001264960.1
Canis lupus familiaris (dog)	DAPK1	NP_001184086.1
Mus musculus (house mouse)	Dapk1	NP_598823.1
Caenorhabditis elegans (roundworm)	dapk-1	NP_490840.2

Gene Ontology
Bibliography

Related articles in PubMed

A novel inducible mutagenesis screen enables to isolate and clone both embryonic and adult zebrafish mutants.
Ma Z, Zhu P, Pang M, Guo L, Chang N, Zheng J, Zhu X, Gao C, Huang H, Cui Z, Xiong JW, Peng J, Chen J
Scientific reports7(1)10381(2017 Sep)

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Evolutionary and developmental analysis reveals KANK genes were co-opted for vertebrate vascular development.
Hensley MR, Cui Z, Chua RF, Simpson S, Shammas NL, Yang JY, Leung YF, Zhang G
Scientific reports627816(2016 Jun)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Interaction of infectious spleen and kidney necrosis virus ORF119L with PINCH leads to dominant-negative inhibition of integrin-linked kinase and cardiovascular defects in zebrafish.
Yuan JM, He BL, Yang LY, Guo CJ, Weng SP, Li SC, He JG
Journal of virology89(1)763-75(2015 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following dapk1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dapk1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa59088	XM_017356102.1 Latest version!	Danio rerio death-associated protein kinase 1 (dapk1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
ODa10713	NM_001099990.2 Latest version!	Danio rerio death-associated protein kinase 1 (dapk1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa10713	XM_017356101.2 Latest version!	Danio rerio death-associated protein kinase 1 (dapk1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa10713	XM_017356100.2 Latest version!	Danio rerio death-associated protein kinase 1 (dapk1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
View Old Accession Versions >>
ODa10713	XM_017356100.1	Danio rerio death-associated protein kinase 1 (dapk1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa10713	XM_017356101.1	Danio rerio death-associated protein kinase 1 (dapk1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dapk1 ( XM_017356102.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017211591.1 Danio rerio death-associated protein kinase 1 (dapk1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa59088
Clone ID Related Accession (Same CDS sequence)	XM_017356102.1
Accession Version	XM_017356102.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2316bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	death-associated protein kinase 1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGTGT TCAACCAGGA GAATGTGGAG GATTATTATG AGATTGGAGG AGAACTCGGG 
AGTGGTCAGT TTGCCGTGGT AAAGAAATGC CGTCATAAGA GCACTGGTGT GGAGTATGCA 
GCCAAATTTA TCAAGAAGAG GCGGAGCAAA TCAAGCCGAC GAGGGGTCTC AAAAGACGAC 
ATTGAGAGGG AGGTCAGCAT CCTGAAGGAA ATCCAGCACC CCAATGTCAT CACCCTGCAC 
GATGTGTTCG AAAACAAGCA TGAAGTCATC CTCATCCTAG AGCTTGTGGC TGGCGGAGAA 
CTCTTTGACT TTCTAGCAGA GAAAGAGTCA CTGAGTGAAG AGGAGGCCAC CGAGTTCCTC 
AAGCAGATCT TGGATGGAGT CAGTTACCTG CACTCGAAAC GGATTTCCCA CTTTGACCTG 
AAGCCGGAGA ACATCATGCT GTTAAACCGG AACGTGCCTC ACCCGCGCAT TAAGCTCATT 
GACTTTGGCC TGGCCCACAA GATCGATTTT GGCAACGATT TCAAAAACAT CTTTGGTACC 
CCGGAGTTTG TAGCTCCAGA AGTGGTGAAC TATGAGCCTC TTGGCCTGGA GGCAGACATG 
TGGAGCATTG GAGTAATCAC TTATATTCTG CTTAGCGGTG CTTCACCTTT CCTGGGTGAA 
AACAAGCAGG AGACGCTTGC CAATGTGTCA GCCGTGGACT ATGAGTTTGA TGAGGACTAT 
TTCAGCCACA CCAGCGCTTT GGCCAAAGAC TTCATTGCAC GGTTACTGAT CAAAGATCCA 
AAAAAAAGAA TGACAATCCT GGACAGTCTT CAACATCCCT GGATCAAGCC AAAGGATACA 
CAGCAGGCAC TGAGTCGCAA AGAATCAGCT GTCAATATGG AGAAATTCAA GAAGTTTGCT 
GCCCGAAGAA AATGGAAGCA ATCAGTGCGT CTCATCTCTC TGTGTAACCG GCTCTCCCGC 
TCCTTTCTGT CCAGGAGCAA CATGAGTGTG GCACGCAGTG ACGACACATT GGATGAAGAG 
GACTCGTTTG TCATGAAGGC TATCATCCAC GCAATAAACG ACGACAATGT TCCCGGTCTG 
AAACACCTAC TTGGATCTCT GACCAGCTAT GATATCAATC AACCTAACAA GCATGGAACT 
CCTCCGCTCC TGATCGCTGC AGGCTGTGGA AACGTCCAGA TCATTGATGT GCTCATGAAA 
AAAGGGGCTG AAATTCAAGC CTTTGATAAG ACTGGAGCCA GTGCTATCTA CTACGCCGCC 
CGACATGGTC ATGTGGGGAC CTTGAGATTC TTGCATGAAA AGAAGTGCCC TCTGGACATC 
CAAGATAAGT CCGGAGAAAC TGCTCTGCAT GTTGCGGCTC GCTATGGTAA TGTGGATGTG 
GTCCAATACC TTTGCAGCAT CCATGCCAAC CCTGACCTGT TGGACCGGGA GCAAGAGACG 
CCTCTCCATT GCGCGGCCTG GCACGGTTAT TCGGCAGTGG CCAGAGCCCT GTGTGAGGCT 
GGCTGTGATG TTAATGCCCG TAACCGTGAG GGTGAGAGTC CACTGCTAAC CGCTTCAGCC 
CGTGGCTTCA AGGACATTGT GGAGTGCTTG CTGGAGCATG GGGCTGACAT GGACTCTGCT 
GATAAGGATG GTCACATCGC TCTGCATCTA GCCGTGAGGA GGTGCCAGGT TGAGGTGGTC 
AAGTGTTTGC TCAGCCATCA CTGCTATGTG GACCACCAGG ATCGTCACGG CAACACACCC 
CTTCACATCG CCTGTAAAGA CGCCAACATG CCTGTGGTCA CGCAGCTCTG TGCTGCCAAA 
GCCAACCTGG ATATTCCCAA CAAATTTGGG AGAACACCTC TTCACCTGGC AGCGAGTAAC 
GGGGGTCTGG AGGTGGTGCG GCATCTCTGT CTTTCAGGAG CAAACACTGA GGCCATCACT 
AATGATGGAA AGACGGCGGA GGATCTGGCC ACTGCAGAGC AGCAGGAGCA TGTAGCAGTG 
CTGCTGGCCA AGCTGAAAAA GGACAACCAT AAGAGCATTT ACATCCAGCA GCTGCGGCCT 
ACACAGACCC TCCAACATCG CCTCAAACTC AAGCTGTTCG GCCATTCTGG AGCAGGGAAG 
AGCACTCTGG TGGAGTCCTT GAAGTGTGGC ATCTTGAGGA GCTTCTTCAG AAGGAGGAGG 
ACCAGGCTGA CCAACCCTGT CCGACACCCA GCCTCGCCGG TCACTTCCAA ACCTGCAGGT 
AGGGGCATGT GGTTTCCCTC TGGATTTAAC ACACCTACAA TGCTTTGCAT GAATTATAAT 
AGTATTGTAC TTGTACATGA GTATTTTTAT AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017211591.1
CDS	266..2581
Translation	MTVFNQENVEDYYEIGGELGSGQFAVVKKCRHKSTGVEYAAKFIKKRRSKSSRRGVSKDDIEREVSILKEIQHPNVITLHDVFENKHEVILILELVAGGELFDFLAEKESLSEEEATEFLKQILDGVSYLHSKRISHFDLKPENIMLLNRNVPHPRIKLIDFGLAHKIDFGNDFKNIFGTPEFVAPEVVNYEPLGLEADMWSIGVITYILLSGASPFLGENKQETLANVSAVDYEFDEDYFSHTSALAKDFIARLLIKDPKKRMTILDSLQHPWIKPKDTQQALSRKESAVNMEKFKKFAARRKWKQSVRLISLCNRLSRSFLSRSNMSVARSDDTLDEEDSFVMKAIIHAINDDNVPGLKHLLGSLTSYDINQPNKHGTPPLLIAAGCGNVQIIDVLMKKGAEIQAFDKTGASAIYYAARHGHVGTLRFLHEKKCPLDIQDKSGETALHVAARYGNVDVVQYLCSIHANPDLLDREQETPLHCAAWHGYSAVARALCEAGCDVNARNREGESPLLTASARGFKDIVECLLEHGADMDSADKDGHIALHLAVRRCQVEVVKCLLSHHCYVDHQDRHGNTPLHIACKDANMPVVTQLCAAKANLDIPNKFGRTPLHLAASNGGLEVVRHLCLSGANTEAITNDGKTAEDLATAEQQEHVAVLLAKLKKDNHKSIYIQQLRPTQTLQHRLKLKLFGHSGAGKSTLVESLKCGILRSFFRRRRTRLTNPVRHPASPVTSKPAGRGMWFPSGFNTPTMLCMNYNSIVLVHEYFYK

Target ORF information:

RefSeq Version	XM_017356102.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio death-associated protein kinase 1 (dapk1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356102.1

ORF Insert Sequence:

ATGACCGTGT TCAACCAGGA GAATGTGGAG GATTATTATG AGATTGGAGG AGAACTCGGG 
AGTGGTCAGT TTGCCGTGGT AAAGAAATGC CGTCATAAGA GCACTGGTGT GGAGTATGCA 
GCCAAATTTA TCAAGAAGAG GCGGAGCAAA TCAAGCCGAC GAGGGGTCTC AAAAGACGAC 
ATTGAGAGGG AGGTCAGCAT CCTGAAGGAA ATCCAGCACC CCAATGTCAT CACCCTGCAC 
GATGTGTTCG AAAACAAGCA TGAAGTCATC CTCATCCTAG AGCTTGTGGC TGGCGGAGAA 
CTCTTTGACT TTCTAGCAGA GAAAGAGTCA CTGAGTGAAG AGGAGGCCAC CGAGTTCCTC 
AAGCAGATCT TGGATGGAGT CAGTTACCTG CACTCGAAAC GGATTTCCCA CTTTGACCTG 
AAGCCGGAGA ACATCATGCT GTTAAACCGG AACGTGCCTC ACCCGCGCAT TAAGCTCATT 
GACTTTGGCC TGGCCCACAA GATCGATTTT GGCAACGATT TCAAAAACAT CTTTGGTACC 
CCGGAGTTTG TAGCTCCAGA AGTGGTGAAC TATGAGCCTC TTGGCCTGGA GGCAGACATG 
TGGAGCATTG GAGTAATCAC TTATATTCTG CTTAGCGGTG CTTCACCTTT CCTGGGTGAA 
AACAAGCAGG AGACGCTTGC CAATGTGTCA GCCGTGGACT ATGAGTTTGA TGAGGACTAT 
TTCAGCCACA CCAGCGCTTT GGCCAAAGAC TTCATTGCAC GGTTACTGAT CAAAGATCCA 
AAAAAAAGAA TGACAATCCT GGACAGTCTT CAACATCCCT GGATCAAGCC AAAGGATACA 
CAGCAGGCAC TGAGTCGCAA AGAATCAGCT GTCAATATGG AGAAATTCAA GAAGTTTGCT 
GCCCGAAGAA AATGGAAGCA ATCAGTGCGT CTCATCTCTC TGTGTAACCG GCTCTCCCGC 
TCCTTTCTGT CCAGGAGCAA CATGAGTGTG GCACGCAGTG ACGACACATT GGATGAAGAG 
GACTCGTTTG TCATGAAGGC TATCATCCAC GCAATAAACG ACGACAATGT TCCCGGTCTG 
AAACACCTAC TTGGATCTCT GACCAGCTAT GATATCAATC AACCTAACAA GCATGGAACT 
CCTCCGCTCC TGATCGCTGC AGGCTGTGGA AACGTCCAGA TCATTGATGT GCTCATGAAA 
AAAGGGGCTG AAATTCAAGC CTTTGATAAG ACTGGAGCCA GTGCTATCTA CTACGCCGCC 
CGACATGGTC ATGTGGGGAC CTTGAGATTC TTGCATGAAA AGAAGTGCCC TCTGGACATC 
CAAGATAAGT CCGGAGAAAC TGCTCTGCAT GTTGCGGCTC GCTATGGTAA TGTGGATGTG 
GTCCAATACC TTTGCAGCAT CCATGCCAAC CCTGACCTGT TGGACCGGGA GCAAGAGACG 
CCTCTCCATT GCGCGGCCTG GCACGGTTAT TCGGCAGTGG CCAGAGCCCT GTGTGAGGCT 
GGCTGTGATG TTAATGCCCG TAACCGTGAG GGTGAGAGTC CACTGCTAAC CGCTTCAGCC 
CGTGGCTTCA AGGACATTGT GGAGTGCTTG CTGGAGCATG GGGCTGACAT GGACTCTGCT 
GATAAGGATG GTCACATCGC TCTGCATCTA GCCGTGAGGA GGTGCCAGGT TGAGGTGGTC 
AAGTGTTTGC TCAGCCATCA CTGCTATGTG GACCACCAGG ATCGTCACGG CAACACACCC 
CTTCACATCG CCTGTAAAGA CGCCAACATG CCTGTGGTCA CGCAGCTCTG TGCTGCCAAA 
GCCAACCTGG ATATTCCCAA CAAATTTGGG AGAACACCTC TTCACCTGGC AGCGAGTAAC 
GGGGGTCTGG AGGTGGTGCG GCATCTCTGT CTTTCAGGAG CAAACACTGA GGCCATCACT 
AATGATGGAA AGACGGCGGA GGATCTGGCC ACTGCAGAGC AGCAGGAGCA TGTAGCAGTG 
CTGCTGGCCA AGCTGAAAAA GGACAACCAT AAGAGCATTT ACATCCAGCA GCTGCGGCCT 
ACACAGACCC TCCAACATCG CCTCAAACTC AAGCTGTTCG GCCATTCTGG AGCAGGGAAG 
AGCACTCTGG TGGAGTCCTT GAAGTGTGGC ATCTTGAGGA GCTTCTTCAG AAGGAGGAGG 
ACCAGGCTGA CCAACCCTGT CCGACACCCA GCCTCGCCGG TCACTTCCAA ACCTGCAGGT 
AGGGGCATGT GGTTTCCCTC TGGATTTAAC ACACCTACAA TGCTTTGCAT GAATTATAAT 
AGTATTGTAC TTGTACATGA GTATTTTTAT AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dapk1 ( NM_001099990.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001093460.1 Danio rerio death-associated protein kinase 1 (dapk1), mRNA.

Price & Availability↑

CloneID	ODa10713
Clone ID Related Accession (Same CDS sequence)	NM_001099990.2 , XM_017356101.1 , XM_017356100.2 , XM_017356100.1 , XM_017356101.2
Accession Version	NM_001099990.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4320bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	death-associated protein kinase 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BX005015.5 and CT025745.13. On May 10, 2008 this sequence version replaced NM_001099990.1. ##Evidence-Data-START## Transcript exon combination :: EU489822.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGACCGTGT TCAACCAGGA GAATGTGGAG GATTATTATG AGATTGGAGG AGAACTCGGG 
AGTGGTCAGT TTGCCGTGGT AAAGAAATGC CGTCATAAGA GCACTGGTGT GGAGTATGCA 
GCCAAATTTA TCAAGAAGAG GCGGAGCAAA TCAAGCCGAC GAGGGGTCTC AAAAGACGAC 
ATTGAGAGGG AGGTCAGCAT CCTGAAGGAA ATCCAGCACC CCAATGTCAT CACCCTGCAC 
GATGTGTTCG AAAACAAGCA TGAAGTCATC CTCATCCTAG AGCTTGTGGC TGGCGGAGAA 
CTCTTTGACT TTCTAGCAGA GAAAGAGTCA CTGAGTGAAG AGGAGGCCAC CGAGTTCCTC 
AAGCAGATCT TGGATGGAGT CAGTTACCTG CACTCGAAAC GGATTTCCCA CTTTGACCTG 
AAGCCGGAGA ACATCATGCT GTTAAACCGG AACGTGCCTC ACCCGCGCAT TAAGCTCATT 
GACTTTGGCC TGGCCCACAA GATCGATTTT GGCAACGATT TCAAAAACAT CTTTGGTACC 
CCGGAGTTTG TAGCTCCAGA AGTGGTGAAC TATGAGCCTC TTGGCCTGGA GGCAGACATG 
TGGAGCATTG GAGTAATCAC TTATATTCTG CTTAGCGGTG CTTCACCTTT CCTGGGTGAA 
AACAAGCAGG AGACGCTTGC CAATGTGTCA GCCGTGGACT ATGAGTTTGA TGAGGACTAT 
TTCAGCCACA CCAGCGCTTT GGCCAAAGAC TTCATTGCAC GGTTACTGAT CAAAGATCCA 
AAAAAAAGAA TGACAATCCT GGACAGTCTT CAACATCCCT GGATCAAGCC AAAGGATACA 
CAGCAGGCAC TGAGTCGCAA AGAATCAGCT GTCAATATGG AGAAATTCAA GAAGTTTGCT 
GCCCGAAGAA AATGGAAGCA ATCAGTGCGT CTCATCTCTC TGTGTAACCG GCTCTCCCGC 
TCCTTTCTGT CCAGGAGCAA CATGAGTGTG GCACGCAGTG ACGACACATT GGATGAAGAG 
GACTCGTTTG TCATGAAGGC TATCATCCAC GCAATAAACG ACGACAATGT TCCCGGTCTG 
AAACACCTAC TTGGATCTCT GACCAGCTAT GATATCAATC AACCTAACAA GCATGGAACT 
CCTCCGCTCC TGATCGCTGC AGGCTGTGGA AACGTCCAGA TCATTGATGT GCTCATGAAA 
AAAGGGGCTG AAATTCAAGC CTTTGATAAG ACTGGAGCCA GTGCTATCTA CTACGCCGCC 
CGACATGGTC ATGTGGGGAC CTTGAGATTC TTGCATGAAA AGAAGTGCCC TCTGGACATC 
CAAGATAAGT CCGGAGAAAC TGCTCTGCAT GTTGCGGCTC GCTATGGTAA TGTGGATGTG 
GTCCAATACC TTTGCAGCAT CCATGCCAAC CCTGACCTGT TGGACCGGGA GCAAGAGACG 
CCTCTCCATT GCGCGGCCTG GCACGGTTAT TCGGCAGTGG CCAGAGCCCT GTGTGAGGCT 
GGCTGTGATG TTAATGCCCG TAACCGTGAG GGTGAGAGTC CACTGCTAAC CGCTTCAGCC 
CGTGGCTTCA AGGACATTGT GGAGTGCTTG CTGGAGCATG GGGCTGACAT GGACTCTGCT 
GATAAGGATG GTCACATCGC TCTGCATCTA GCCGTGAGGA GGTGCCAGGT TGAGGTGGTC 
AAGTGTTTGC TCAGCCATCA CTGCTATGTG GACCACCAGG ATCGTCACGG CAACACACCC 
CTTCACATCG CCTGTAAAGA CGCCAACATG CCTGTGGTCA CGCAGCTCTG TGCTGCCAAA 
GCCAACCTGG ATATTCCCAA CAAATTTGGG AGAACACCTC TTCACCTGGC AGCGAGTAAC 
GGGGGTCTGG AGGTGGTGCG GCATCTCTGT CTTTCAGGAG CAAACACTGA GGCCATCACT 
AATGATGGAA AGACGGCGGA GGATCTGGCC ACTGCAGAGC AGCAGGAGCA TGTAGCAGTG 
CTGCTGGCCA AGCTGAAAAA GGACAACCAT AAGAGCATTT ACATCCAGCA GCTGCGGCCT 
ACACAGACCC TCCAACATCG CCTCAAACTC AAGCTGTTCG GCCATTCTGG AGCAGGGAAG 
AGCACTCTGG TGGAGTCCTT GAAGTGTGGC ATCTTGAGGA GCTTCTTCAG AAGGAGGAGG 
ACCAGGCTGA CCAACCCTGT CCGACACCCA GCCTCGCCGG TCACTTCCAA ACCTGCAGTG 
TCTGTGAGCA TCTCTAACCT GTATCCGGGG TGTGAGAATG TGAGTGTTCG GAGCCGCAGT 
ATGATGTTCG AGCCCAGTTT GACTAAAGGA GTGCTGGAGG TCTTCAGTCC TGTTCATAAT 
GCTCTCTCCA CGGCTGACGA CACTGCCACT AAAGCCATCG ACATCCAGAA CGCCAACGTC 
AATGGTGTTG GAGACTTCAG TATATGGGAG TTTTCTGGCA ATCCCGTCTA TTATTGCTCG 
TATGACTACT TCGCTGCCAA TGATGCCACA GCGATTCATC TGGTCTTATT CAGCCTGGAG 
GAGCCCTACG AAACTCAGCT CAACCAGGTC ACATTCTGGC TGAACTGTCT AAAGGCTCTC 
ACGCTGCCAG AGGACAACAT CGCCTTTGGA GGGAAGATCA GGAATCCTCT GCGTGTGGTT 
CTGGTGGCCA CACATGCTGA CATTGTGAAT CTGCCCCGAT CGTTTGGAGG AGAGTTCAGC 
TATGACAAGG AGAGATCTCT ACTGAAGGAA GTCAGAAACA GGTTTGGAAA TGATCTGCTG 
ATCACTGAGA AGCTCTTTGT CATGGATGCA GGAGCGTCCA ACTCTAAAGA CATAAAGCTT 
CTGAGAAACC TTCTGTTTGA GCTCCGCAGC ACCATCATTT CTACCTGCAG GCCGATGACT 
CAGCTGGCAG AGAAGCTGTT GTCCACTCTG CCTTCCTGGA GGAAACTGAA CGGGCCGAAC 
CAGCTCATAT CATGGCAACA GTTTGTTGTG GATGTGCAAG AACAAATCAA CCCTCTAGCC 
AGCGAAGAGG ATCTGCGGGG ACTGGCTTCA CAACTTCACA GCATGGGAGA GATTAACATC 
ATGCAGAGTG AGACAGTCCA AGATGTTGTC CTCTTAGATC CTCGCTGGCT CTGTAGTAAC 
GTACTTGGCA AGCTTCTCTC AATTGAAACC CCCAAAGCAA TCCACCATTA TCGCGGCCGA 
TACCGTGTAG AAGAGCTCCA ACACCTGGTC TCCGAAGGCG ACATCGATGA ACTTCTCCAA 
GTCCTCGACG CCATGGACGT CTGCGCCCGC GACCTGGCCA ACCCAGGAAT GGTGGACATC 
CCTGCCCTCA TTCGCACAGG TGGCCTGCAG CATACCTGGA CAGACGAGGA GGACGTGGAG 
GGAGAGGACG CTCTGCTTTA CGGTGGAGTC CGTATGGTTC CTGTGGAGCA CCTGGCACCC 
TTTCCTTGCG GACTTTTCCA TAAGTTACAG GTGAACTTGT GCAGGTGGAG CCGCCAGCAG 
AAGCCTGAGG TGGAGGCTGA TGATGTGAGG TTGTGGAGCG GTGGCGCTCG CATCGGACTT 
GGTGGTGTGG AGGCTCTGGT TCTGCTTGTG AACCACGGGC AAGGCATGGA GGTACAGTTA 
CGAGGACCTG ACACAGATCC GGGACGCTGC TATGCTTTGT TGGACACTCT GTGCGGCATG 
GCTGAGGGTC TGTTAGCTGC TACTTTGCCC GGTTTGCTTA CCGCCCGACA CTATCTCAGC 
CCTCAGCAAC TGCGTGAACA CCACGGCCCA GTCATGCTTT ACCAGCCGAG GGACTTTTTT 
AGAGCTCAAG CGCGAGGTGA GAACGCTCTG TCGAACACCA TGGGTGGCTA TCGCGAAAGC 
TTTGTCAGTA TCGTAGCATT AGGATGCATT CATGTCTACA AACAGGGCAA CCTCGGTGGC 
GAAGTGGCTG TAGCAGAGGT CAGTCTTTTG GCTCGACGTA AGCTCTGCCG CCTGCTAGAC 
CCGCCTGATG CCCTGGGCAA GGACTGGTGC CTGTTGGCCA TGAACTTGGG CCTTAGCGAA 
CTAGCTGCTA AATACAGTTC TGGCATCAAC GGGACTCCAC CAGCAGACCC GCTTTCTCTC 
CCTAGCCCTA CAGCCACACT CCTGCAGGAG TGGAGCCAGA GGCCTGACTC AACCATTGGT 
GTTTTGGTTG CAAAGTTGCG AGAACTTGGC CGTCGAGACG CGGCAGATTT CCTGCTTAGA 
GCTGCACCAG TGTTTCGGGT GAACTTGGAG ATTTTGGGCA CCCCGTCTGA GCCCTACAGC 
CCCGTGTGTA ATGGAGGAGG CACATCCTAC AACTCCATCA GCTCCGTCAT ATCTCGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001093460.1
CDS	1..4320
Translation	MTVFNQENVEDYYEIGGELGSGQFAVVKKCRHKSTGVEYAAKFIKKRRSKSSRRGVSKDDIEREVSILKEIQHPNVITLHDVFENKHEVILILELVAGGELFDFLAEKESLSEEEATEFLKQILDGVSYLHSKRISHFDLKPENIMLLNRNVPHPRIKLIDFGLAHKIDFGNDFKNIFGTPEFVAPEVVNYEPLGLEADMWSIGVITYILLSGASPFLGENKQETLANVSAVDYEFDEDYFSHTSALAKDFIARLLIKDPKKRMTILDSLQHPWIKPKDTQQALSRKESAVNMEKFKKFAARRKWKQSVRLISLCNRLSRSFLSRSNMSVARSDDTLDEEDSFVMKAIIHAINDDNVPGLKHLLGSLTSYDINQPNKHGTPPLLIAAGCGNVQIIDVLMKKGAEIQAFDKTGASAIYYAARHGHVGTLRFLHEKKCPLDIQDKSGETALHVAARYGNVDVVQYLCSIHANPDLLDREQETPLHCAAWHGYSAVARALCEAGCDVNARNREGESPLLTASARGFKDIVECLLEHGADMDSADKDGHIALHLAVRRCQVEVVKCLLSHHCYVDHQDRHGNTPLHIACKDANMPVVTQLCAAKANLDIPNKFGRTPLHLAASNGGLEVVRHLCLSGANTEAITNDGKTAEDLATAEQQEHVAVLLAKLKKDNHKSIYIQQLRPTQTLQHRLKLKLFGHSGAGKSTLVESLKCGILRSFFRRRRTRLTNPVRHPASPVTSKPAVSVSISNLYPGCENVSVRSRSMMFEPSLTKGVLEVFSPVHNALSTADDTATKAIDIQNANVNGVGDFSIWEFSGNPVYYCSYDYFAANDATAIHLVLFSLEEPYETQLNQVTFWLNCLKALTLPEDNIAFGGKIRNPLRVVLVATHADIVNLPRSFGGEFSYDKERSLLKEVRNRFGNDLLITEKLFVMDAGASNSKDIKLLRNLLFELRSTIISTCRPMTQLAEKLLSTLPSWRKLNGPNQLISWQQFVVDVQEQINPLASEEDLRGLASQLHSMGEINIMQSETVQDVVLLDPRWLCSNVLGKLLSIETPKAIHHYRGRYRVEELQHLVSEGDIDELLQVLDAMDVCARDLANPGMVDIPALIRTGGLQHTWTDEEDVEGEDALLYGGVRMVPVEHLAPFPCGLFHKLQVNLCRWSRQQKPEVEADDVRLWSGGARIGLGGVEALVLLVNHGQGMEVQLRGPDTDPGRCYALLDTLCGMAEGLLAATLPGLLTARHYLSPQQLREHHGPVMLYQPRDFFRAQARGENALSNTMGGYRESFVSIVALGCIHVYKQGNLGGEVAVAEVSLLARRKLCRLLDPPDALGKDWCLLAMNLGLSELAAKYSSGINGTPPADPLSLPSPTATLLQEWSQRPDSTIGVLVAKLRELGRRDAADFLLRAAPVFRVNLEILGTPSEPYSPVCNGGGTSYNSISSVISR

Target ORF information:

RefSeq Version	NM_001099990.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio death-associated protein kinase 1 (dapk1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001099990.2

ORF Insert Sequence:

ATGACCGTGT TCAACCAGGA GAATGTGGAG GATTATTATG AGATTGGAGG AGAACTCGGG 
AGTGGTCAGT TTGCCGTGGT AAAGAAATGC CGTCATAAGA GCACTGGTGT GGAGTATGCA 
GCCAAATTTA TCAAGAAGAG GCGGAGCAAA TCAAGCCGAC GAGGGGTCTC AAAAGACGAC 
ATTGAGAGGG AGGTCAGCAT CCTGAAGGAA ATCCAGCACC CCAATGTCAT CACCCTGCAC 
GATGTGTTCG AAAACAAGCA TGAAGTCATC CTCATCCTAG AGCTTGTGGC TGGCGGAGAA 
CTCTTTGACT TTCTAGCAGA GAAAGAGTCA CTGAGTGAAG AGGAGGCCAC CGAGTTCCTC 
AAGCAGATCT TGGATGGAGT CAGTTACCTG CACTCGAAAC GGATTTCCCA CTTTGACCTG 
AAGCCGGAGA ACATCATGCT GTTAAACCGG AACGTGCCTC ACCCGCGCAT TAAGCTCATT 
GACTTTGGCC TGGCCCACAA GATCGATTTT GGCAACGATT TCAAAAACAT CTTTGGTACC 
CCGGAGTTTG TAGCTCCAGA AGTGGTGAAC TATGAGCCTC TTGGCCTGGA GGCAGACATG 
TGGAGCATTG GAGTAATCAC TTATATTCTG CTTAGCGGTG CTTCACCTTT CCTGGGTGAA 
AACAAGCAGG AGACGCTTGC CAATGTGTCA GCCGTGGACT ATGAGTTTGA TGAGGACTAT 
TTCAGCCACA CCAGCGCTTT GGCCAAAGAC TTCATTGCAC GGTTACTGAT CAAAGATCCA 
AAAAAAAGAA TGACAATCCT GGACAGTCTT CAACATCCCT GGATCAAGCC AAAGGATACA 
CAGCAGGCAC TGAGTCGCAA AGAATCAGCT GTCAATATGG AGAAATTCAA GAAGTTTGCT 
GCCCGAAGAA AATGGAAGCA ATCAGTGCGT CTCATCTCTC TGTGTAACCG GCTCTCCCGC 
TCCTTTCTGT CCAGGAGCAA CATGAGTGTG GCACGCAGTG ACGACACATT GGATGAAGAG 
GACTCGTTTG TCATGAAGGC TATCATCCAC GCAATAAACG ACGACAATGT TCCCGGTCTG 
AAACACCTAC TTGGATCTCT GACCAGCTAT GATATCAATC AACCTAACAA GCATGGAACT 
CCTCCGCTCC TGATCGCTGC AGGCTGTGGA AACGTCCAGA TCATTGATGT GCTCATGAAA 
AAAGGGGCTG AAATTCAAGC CTTTGATAAG ACTGGAGCCA GTGCTATCTA CTACGCCGCC 
CGACATGGTC ATGTGGGGAC CTTGAGATTC TTGCATGAAA AGAAGTGCCC TCTGGACATC 
CAAGATAAGT CCGGAGAAAC TGCTCTGCAT GTTGCGGCTC GCTATGGTAA TGTGGATGTG 
GTCCAATACC TTTGCAGCAT CCATGCCAAC CCTGACCTGT TGGACCGGGA GCAAGAGACG 
CCTCTCCATT GCGCGGCCTG GCACGGTTAT TCGGCAGTGG CCAGAGCCCT GTGTGAGGCT 
GGCTGTGATG TTAATGCCCG TAACCGTGAG GGTGAGAGTC CACTGCTAAC CGCTTCAGCC 
CGTGGCTTCA AGGACATTGT GGAGTGCTTG CTGGAGCATG GGGCTGACAT GGACTCTGCT 
GATAAGGATG GTCACATCGC TCTGCATCTA GCCGTGAGGA GGTGCCAGGT TGAGGTGGTC 
AAGTGTTTGC TCAGCCATCA CTGCTATGTG GACCACCAGG ATCGTCACGG CAACACACCC 
CTTCACATCG CCTGTAAAGA CGCCAACATG CCTGTGGTCA CGCAGCTCTG TGCTGCCAAA 
GCCAACCTGG ATATTCCCAA CAAATTTGGG AGAACACCTC TTCACCTGGC AGCGAGTAAC 
GGGGGTCTGG AGGTGGTGCG GCATCTCTGT CTTTCAGGAG CAAACACTGA GGCCATCACT 
AATGATGGAA AGACGGCGGA GGATCTGGCC ACTGCAGAGC AGCAGGAGCA TGTAGCAGTG 
CTGCTGGCCA AGCTGAAAAA GGACAACCAT AAGAGCATTT ACATCCAGCA GCTGCGGCCT 
ACACAGACCC TCCAACATCG CCTCAAACTC AAGCTGTTCG GCCATTCTGG AGCAGGGAAG 
AGCACTCTGG TGGAGTCCTT GAAGTGTGGC ATCTTGAGGA GCTTCTTCAG AAGGAGGAGG 
ACCAGGCTGA CCAACCCTGT CCGACACCCA GCCTCGCCGG TCACTTCCAA ACCTGCAGTG 
TCTGTGAGCA TCTCTAACCT GTATCCGGGG TGTGAGAATG TGAGTGTTCG GAGCCGCAGT 
ATGATGTTCG AGCCCAGTTT GACTAAAGGA GTGCTGGAGG TCTTCAGTCC TGTTCATAAT 
GCTCTCTCCA CGGCTGACGA CACTGCCACT AAAGCCATCG ACATCCAGAA CGCCAACGTC 
AATGGTGTTG GAGACTTCAG TATATGGGAG TTTTCTGGCA ATCCCGTCTA TTATTGCTCG 
TATGACTACT TCGCTGCCAA TGATGCCACA GCGATTCATC TGGTCTTATT CAGCCTGGAG 
GAGCCCTACG AAACTCAGCT CAACCAGGTC ACATTCTGGC TGAACTGTCT AAAGGCTCTC 
ACGCTGCCAG AGGACAACAT CGCCTTTGGA GGGAAGATCA GGAATCCTCT GCGTGTGGTT 
CTGGTGGCCA CACATGCTGA CATTGTGAAT CTGCCCCGAT CGTTTGGAGG AGAGTTCAGC 
TATGACAAGG AGAGATCTCT ACTGAAGGAA GTCAGAAACA GGTTTGGAAA TGATCTGCTG 
ATCACTGAGA AGCTCTTTGT CATGGATGCA GGAGCGTCCA ACTCTAAAGA CATAAAGCTT 
CTGAGAAACC TTCTGTTTGA GCTCCGCAGC ACCATCATTT CTACCTGCAG GCCGATGACT 
CAGCTGGCAG AGAAGCTGTT GTCCACTCTG CCTTCCTGGA GGAAACTGAA CGGGCCGAAC 
CAGCTCATAT CATGGCAACA GTTTGTTGTG GATGTGCAAG AACAAATCAA CCCTCTAGCC 
AGCGAAGAGG ATCTGCGGGG ACTGGCTTCA CAACTTCACA GCATGGGAGA GATTAACATC 
ATGCAGAGTG AGACAGTCCA AGATGTTGTC CTCTTAGATC CTCGCTGGCT CTGTAGTAAC 
GTACTTGGCA AGCTTCTCTC AATTGAAACC CCCAAAGCAA TCCACCATTA TCGCGGCCGA 
TACCGTGTAG AAGAGCTCCA ACACCTGGTC TCCGAAGGCG ACATCGATGA ACTTCTCCAA 
GTCCTCGACG CCATGGACGT CTGCGCCCGC GACCTGGCCA ACCCAGGAAT GGTGGACATC 
CCTGCCCTCA TTCGCACAGG TGGCCTGCAG CATACCTGGA CAGACGAGGA GGACGTGGAG 
GGAGAGGACG CTCTGCTTTA CGGTGGAGTC CGTATGGTTC CTGTGGAGCA CCTGGCACCC 
TTTCCTTGCG GACTTTTCCA TAAGTTACAG GTGAACTTGT GCAGGTGGAG CCGCCAGCAG 
AAGCCTGAGG TGGAGGCTGA TGATGTGAGG TTGTGGAGCG GTGGCGCTCG CATCGGACTT 
GGTGGTGTGG AGGCTCTGGT TCTGCTTGTG AACCACGGGC AAGGCATGGA GGTACAGTTA 
CGAGGACCTG ACACAGATCC GGGACGCTGC TATGCTTTGT TGGACACTCT GTGCGGCATG 
GCTGAGGGTC TGTTAGCTGC TACTTTGCCC GGTTTGCTTA CCGCCCGACA CTATCTCAGC 
CCTCAGCAAC TGCGTGAACA CCACGGCCCA GTCATGCTTT ACCAGCCGAG GGACTTTTTT 
AGAGCTCAAG CGCGAGGTGA GAACGCTCTG TCGAACACCA TGGGTGGCTA TCGCGAAAGC 
TTTGTCAGTA TCGTAGCATT AGGATGCATT CATGTCTACA AACAGGGCAA CCTCGGTGGC 
GAAGTGGCTG TAGCAGAGGT CAGTCTTTTG GCTCGACGTA AGCTCTGCCG CCTGCTAGAC 
CCGCCTGATG CCCTGGGCAA GGACTGGTGC CTGTTGGCCA TGAACTTGGG CCTTAGCGAA 
CTAGCTGCTA AATACAGTTC TGGCATCAAC GGGACTCCAC CAGCAGACCC GCTTTCTCTC 
CCTAGCCCTA CAGCCACACT CCTGCAGGAG TGGAGCCAGA GGCCTGACTC AACCATTGGT 
GTTTTGGTTG CAAAGTTGCG AGAACTTGGC CGTCGAGACG CGGCAGATTT CCTGCTTAGA 
GCTGCACCAG TGTTTCGGGT GAACTTGGAG ATTTTGGGCA CCCCGTCTGA GCCCTACAGC 
CCCGTGTGTA ATGGAGGAGG CACATCCTAC AACTCCATCA GCTCCGTCAT ATCTCGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dapk1 ( XM_017356101.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017211590.1 Danio rerio death-associated protein kinase 1 (dapk1), transcript variant X2, mRNA.

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CloneID	ODa10713
Clone ID Related Accession (Same CDS sequence)	NM_001099990.2 , XM_017356101.1 , XM_017356100.2 , XM_017356100.1 , XM_017356101.2
Accession Version	XM_017356101.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4320bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	death-associated protein kinase 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGTGT TCAACCAGGA GAATGTGGAG GATTATTATG AGATTGGAGG AGAACTCGGG 
AGTGGTCAGT TTGCCGTGGT AAAGAAATGC CGTCATAAGA GCACTGGTGT GGAGTATGCA 
GCCAAATTTA TCAAGAAGAG GCGGAGCAAA TCAAGCCGAC GAGGGGTCTC AAAAGACGAC 
ATTGAGAGGG AGGTCAGCAT CCTGAAGGAA ATCCAGCACC CCAATGTCAT CACCCTGCAC 
GATGTGTTCG AAAACAAGCA TGAAGTCATC CTCATCCTAG AGCTTGTGGC TGGCGGAGAA 
CTCTTTGACT TTCTAGCAGA GAAAGAGTCA CTGAGTGAAG AGGAGGCCAC CGAGTTCCTC 
AAGCAGATCT TGGATGGAGT CAGTTACCTG CACTCGAAAC GGATTTCCCA CTTTGACCTG 
AAGCCGGAGA ACATCATGCT GTTAAACCGG AACGTGCCTC ACCCGCGCAT TAAGCTCATT 
GACTTTGGCC TGGCCCACAA GATCGATTTT GGCAACGATT TCAAAAACAT CTTTGGTACC 
CCGGAGTTTG TAGCTCCAGA AGTGGTGAAC TATGAGCCTC TTGGCCTGGA GGCAGACATG 
TGGAGCATTG GAGTAATCAC TTATATTCTG CTTAGCGGTG CTTCACCTTT CCTGGGTGAA 
AACAAGCAGG AGACGCTTGC CAATGTGTCA GCCGTGGACT ATGAGTTTGA TGAGGACTAT 
TTCAGCCACA CCAGCGCTTT GGCCAAAGAC TTCATTGCAC GGTTACTGAT CAAAGATCCA 
AAAAAAAGAA TGACAATCCT GGACAGTCTT CAACATCCCT GGATCAAGCC AAAGGATACA 
CAGCAGGCAC TGAGTCGCAA AGAATCAGCT GTCAATATGG AGAAATTCAA GAAGTTTGCT 
GCCCGAAGAA AATGGAAGCA ATCAGTGCGT CTCATCTCTC TGTGTAACCG GCTCTCCCGC 
TCCTTTCTGT CCAGGAGCAA CATGAGTGTG GCACGCAGTG ACGACACATT GGATGAAGAG 
GACTCGTTTG TCATGAAGGC TATCATCCAC GCAATAAACG ACGACAATGT TCCCGGTCTG 
AAACACCTAC TTGGATCTCT GACCAGCTAT GATATCAATC AACCTAACAA GCATGGAACT 
CCTCCGCTCC TGATCGCTGC AGGCTGTGGA AACGTCCAGA TCATTGATGT GCTCATGAAA 
AAAGGGGCTG AAATTCAAGC CTTTGATAAG ACTGGAGCCA GTGCTATCTA CTACGCCGCC 
CGACATGGTC ATGTGGGGAC CTTGAGATTC TTGCATGAAA AGAAGTGCCC TCTGGACATC 
CAAGATAAGT CCGGAGAAAC TGCTCTGCAT GTTGCGGCTC GCTATGGTAA TGTGGATGTG 
GTCCAATACC TTTGCAGCAT CCATGCCAAC CCTGACCTGT TGGACCGGGA GCAAGAGACG 
CCTCTCCATT GCGCGGCCTG GCACGGTTAT TCGGCAGTGG CCAGAGCCCT GTGTGAGGCT 
GGCTGTGATG TTAATGCCCG TAACCGTGAG GGTGAGAGTC CACTGCTAAC CGCTTCAGCC 
CGTGGCTTCA AGGACATTGT GGAGTGCTTG CTGGAGCATG GGGCTGACAT GGACTCTGCT 
GATAAGGATG GTCACATCGC TCTGCATCTA GCCGTGAGGA GGTGCCAGGT TGAGGTGGTC 
AAGTGTTTGC TCAGCCATCA CTGCTATGTG GACCACCAGG ATCGTCACGG CAACACACCC 
CTTCACATCG CCTGTAAAGA CGCCAACATG CCTGTGGTCA CGCAGCTCTG TGCTGCCAAA 
GCCAACCTGG ATATTCCCAA CAAATTTGGG AGAACACCTC TTCACCTGGC AGCGAGTAAC 
GGGGGTCTGG AGGTGGTGCG GCATCTCTGT CTTTCAGGAG CAAACACTGA GGCCATCACT 
AATGATGGAA AGACGGCGGA GGATCTGGCC ACTGCAGAGC AGCAGGAGCA TGTAGCAGTG 
CTGCTGGCCA AGCTGAAAAA GGACAACCAT AAGAGCATTT ACATCCAGCA GCTGCGGCCT 
ACACAGACCC TCCAACATCG CCTCAAACTC AAGCTGTTCG GCCATTCTGG AGCAGGGAAG 
AGCACTCTGG TGGAGTCCTT GAAGTGTGGC ATCTTGAGGA GCTTCTTCAG AAGGAGGAGG 
ACCAGGCTGA CCAACCCTGT CCGACACCCA GCCTCGCCGG TCACTTCCAA ACCTGCAGTG 
TCTGTGAGCA TCTCTAACCT GTATCCGGGG TGTGAGAATG TGAGTGTTCG GAGCCGCAGT 
ATGATGTTCG AGCCCAGTTT GACTAAAGGA GTGCTGGAGG TCTTCAGTCC TGTTCATAAT 
GCTCTCTCCA CGGCTGACGA CACTGCCACT AAAGCCATCG ACATCCAGAA CGCCAACGTC 
AATGGTGTTG GAGACTTCAG TATATGGGAG TTTTCTGGCA ATCCCGTCTA TTATTGCTCG 
TATGACTACT TCGCTGCCAA TGATGCCACA GCGATTCATC TGGTCTTATT CAGCCTGGAG 
GAGCCCTACG AAACTCAGCT CAACCAGGTC ACATTCTGGC TGAACTGTCT AAAGGCTCTC 
ACGCTGCCAG AGGACAACAT CGCCTTTGGA GGGAAGATCA GGAATCCTCT GCGTGTGGTT 
CTGGTGGCCA CACATGCTGA CATTGTGAAT CTGCCCCGAT CGTTTGGAGG AGAGTTCAGC 
TATGACAAGG AGAGATCTCT ACTGAAGGAA GTCAGAAACA GGTTTGGAAA TGATCTGCTG 
ATCACTGAGA AGCTCTTTGT CATGGATGCA GGAGCGTCCA ACTCTAAAGA CATAAAGCTT 
CTGAGAAACC TTCTGTTTGA GCTCCGCAGC ACCATCATTT CTACCTGCAG GCCGATGACT 
CAGCTGGCAG AGAAGCTGTT GTCCACTCTG CCTTCCTGGA GGAAACTGAA CGGGCCGAAC 
CAGCTCATAT CATGGCAACA GTTTGTTGTG GATGTGCAAG AACAAATCAA CCCTCTAGCC 
AGCGAAGAGG ATCTGCGGGG ACTGGCTTCA CAACTTCACA GCATGGGAGA GATTAACATC 
ATGCAGAGTG AGACAGTCCA AGATGTTGTC CTCTTAGATC CTCGCTGGCT CTGTAGTAAC 
GTACTTGGCA AGCTTCTCTC AATTGAAACC CCCAAAGCAA TCCACCATTA TCGCGGCCGA 
TACCGTGTAG AAGAGCTCCA ACACCTGGTC TCCGAAGGCG ACATCGATGA ACTTCTCCAA 
GTCCTCGACG CCATGGACGT CTGCGCCCGC GACCTGGCCA ACCCAGGAAT GGTGGACATC 
CCTGCCCTCA TTCGCACAGG TGGCCTGCAG CATACCTGGA CAGACGAGGA GGACGTGGAG 
GGAGAGGACG CTCTGCTTTA CGGTGGAGTC CGTATGGTTC CTGTGGAGCA CCTGGCACCC 
TTTCCTTGCG GACTTTTCCA TAAGTTACAG GTGAACTTGT GCAGGTGGAG CCGCCAGCAG 
AAGCCTGAGG TGGAGGCTGA TGATGTGAGG TTGTGGAGCG GTGGCGCTCG CATCGGACTT 
GGTGGTGTGG AGGCTCTGGT TCTGCTTGTG AACCACGGGC AAGGCATGGA GGTACAGTTA 
CGAGGACCTG ACACAGATCC GGGACGCTGC TATGCTTTGT TGGACACTCT GTGCGGCATG 
GCTGAGGGTC TGTTAGCTGC TACTTTGCCC GGTTTGCTTA CCGCCCGACA CTATCTCAGC 
CCTCAGCAAC TGCGTGAACA CCACGGCCCA GTCATGCTTT ACCAGCCGAG GGACTTTTTT 
AGAGCTCAAG CGCGAGGTGA GAACGCTCTG TCGAACACCA TGGGTGGCTA TCGCGAAAGC 
TTTGTCAGTA TCGTAGCATT AGGATGCATT CATGTCTACA AACAGGGCAA CCTCGGTGGC 
GAAGTGGCTG TAGCAGAGGT CAGTCTTTTG GCTCGACGTA AGCTCTGCCG CCTGCTAGAC 
CCGCCTGATG CCCTGGGCAA GGACTGGTGC CTGTTGGCCA TGAACTTGGG CCTTAGCGAA 
CTAGCTGCTA AATACAGTTC TGGCATCAAC GGGACTCCAC CAGCAGACCC GCTTTCTCTC 
CCTAGCCCTA CAGCCACACT CCTGCAGGAG TGGAGCCAGA GGCCTGACTC AACCATTGGT 
GTTTTGGTTG CAAAGTTGCG AGAACTTGGC CGTCGAGACG CGGCAGATTT CCTGCTTAGA 
GCTGCACCAG TGTTTCGGGT GAACTTGGAG ATTTTGGGCA CCCCGTCTGA GCCCTACAGC 
CCCGTGTGTA ATGGAGGAGG CACATCCTAC AACTCCATCA GCTCCGTCAT ATCTCGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017211590.1
CDS	267..4586
Translation	MTVFNQENVEDYYEIGGELGSGQFAVVKKCRHKSTGVEYAAKFIKKRRSKSSRRGVSKDDIEREVSILKEIQHPNVITLHDVFENKHEVILILELVAGGELFDFLAEKESLSEEEATEFLKQILDGVSYLHSKRISHFDLKPENIMLLNRNVPHPRIKLIDFGLAHKIDFGNDFKNIFGTPEFVAPEVVNYEPLGLEADMWSIGVITYILLSGASPFLGENKQETLANVSAVDYEFDEDYFSHTSALAKDFIARLLIKDPKKRMTILDSLQHPWIKPKDTQQALSRKESAVNMEKFKKFAARRKWKQSVRLISLCNRLSRSFLSRSNMSVARSDDTLDEEDSFVMKAIIHAINDDNVPGLKHLLGSLTSYDINQPNKHGTPPLLIAAGCGNVQIIDVLMKKGAEIQAFDKTGASAIYYAARHGHVGTLRFLHEKKCPLDIQDKSGETALHVAARYGNVDVVQYLCSIHANPDLLDREQETPLHCAAWHGYSAVARALCEAGCDVNARNREGESPLLTASARGFKDIVECLLEHGADMDSADKDGHIALHLAVRRCQVEVVKCLLSHHCYVDHQDRHGNTPLHIACKDANMPVVTQLCAAKANLDIPNKFGRTPLHLAASNGGLEVVRHLCLSGANTEAITNDGKTAEDLATAEQQEHVAVLLAKLKKDNHKSIYIQQLRPTQTLQHRLKLKLFGHSGAGKSTLVESLKCGILRSFFRRRRTRLTNPVRHPASPVTSKPAVSVSISNLYPGCENVSVRSRSMMFEPSLTKGVLEVFSPVHNALSTADDTATKAIDIQNANVNGVGDFSIWEFSGNPVYYCSYDYFAANDATAIHLVLFSLEEPYETQLNQVTFWLNCLKALTLPEDNIAFGGKIRNPLRVVLVATHADIVNLPRSFGGEFSYDKERSLLKEVRNRFGNDLLITEKLFVMDAGASNSKDIKLLRNLLFELRSTIISTCRPMTQLAEKLLSTLPSWRKLNGPNQLISWQQFVVDVQEQINPLASEEDLRGLASQLHSMGEINIMQSETVQDVVLLDPRWLCSNVLGKLLSIETPKAIHHYRGRYRVEELQHLVSEGDIDELLQVLDAMDVCARDLANPGMVDIPALIRTGGLQHTWTDEEDVEGEDALLYGGVRMVPVEHLAPFPCGLFHKLQVNLCRWSRQQKPEVEADDVRLWSGGARIGLGGVEALVLLVNHGQGMEVQLRGPDTDPGRCYALLDTLCGMAEGLLAATLPGLLTARHYLSPQQLREHHGPVMLYQPRDFFRAQARGENALSNTMGGYRESFVSIVALGCIHVYKQGNLGGEVAVAEVSLLARRKLCRLLDPPDALGKDWCLLAMNLGLSELAAKYSSGINGTPPADPLSLPSPTATLLQEWSQRPDSTIGVLVAKLRELGRRDAADFLLRAAPVFRVNLEILGTPSEPYSPVCNGGGTSYNSISSVISR

Target ORF information:

RefSeq Version	XM_017356101.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio death-associated protein kinase 1 (dapk1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356101.1

ORF Insert Sequence:

ATGACCGTGT TCAACCAGGA GAATGTGGAG GATTATTATG AGATTGGAGG AGAACTCGGG 
AGTGGTCAGT TTGCCGTGGT AAAGAAATGC CGTCATAAGA GCACTGGTGT GGAGTATGCA 
GCCAAATTTA TCAAGAAGAG GCGGAGCAAA TCAAGCCGAC GAGGGGTCTC AAAAGACGAC 
ATTGAGAGGG AGGTCAGCAT CCTGAAGGAA ATCCAGCACC CCAATGTCAT CACCCTGCAC 
GATGTGTTCG AAAACAAGCA TGAAGTCATC CTCATCCTAG AGCTTGTGGC TGGCGGAGAA 
CTCTTTGACT TTCTAGCAGA GAAAGAGTCA CTGAGTGAAG AGGAGGCCAC CGAGTTCCTC 
AAGCAGATCT TGGATGGAGT CAGTTACCTG CACTCGAAAC GGATTTCCCA CTTTGACCTG 
AAGCCGGAGA ACATCATGCT GTTAAACCGG AACGTGCCTC ACCCGCGCAT TAAGCTCATT 
GACTTTGGCC TGGCCCACAA GATCGATTTT GGCAACGATT TCAAAAACAT CTTTGGTACC 
CCGGAGTTTG TAGCTCCAGA AGTGGTGAAC TATGAGCCTC TTGGCCTGGA GGCAGACATG 
TGGAGCATTG GAGTAATCAC TTATATTCTG CTTAGCGGTG CTTCACCTTT CCTGGGTGAA 
AACAAGCAGG AGACGCTTGC CAATGTGTCA GCCGTGGACT ATGAGTTTGA TGAGGACTAT 
TTCAGCCACA CCAGCGCTTT GGCCAAAGAC TTCATTGCAC GGTTACTGAT CAAAGATCCA 
AAAAAAAGAA TGACAATCCT GGACAGTCTT CAACATCCCT GGATCAAGCC AAAGGATACA 
CAGCAGGCAC TGAGTCGCAA AGAATCAGCT GTCAATATGG AGAAATTCAA GAAGTTTGCT 
GCCCGAAGAA AATGGAAGCA ATCAGTGCGT CTCATCTCTC TGTGTAACCG GCTCTCCCGC 
TCCTTTCTGT CCAGGAGCAA CATGAGTGTG GCACGCAGTG ACGACACATT GGATGAAGAG 
GACTCGTTTG TCATGAAGGC TATCATCCAC GCAATAAACG ACGACAATGT TCCCGGTCTG 
AAACACCTAC TTGGATCTCT GACCAGCTAT GATATCAATC AACCTAACAA GCATGGAACT 
CCTCCGCTCC TGATCGCTGC AGGCTGTGGA AACGTCCAGA TCATTGATGT GCTCATGAAA 
AAAGGGGCTG AAATTCAAGC CTTTGATAAG ACTGGAGCCA GTGCTATCTA CTACGCCGCC 
CGACATGGTC ATGTGGGGAC CTTGAGATTC TTGCATGAAA AGAAGTGCCC TCTGGACATC 
CAAGATAAGT CCGGAGAAAC TGCTCTGCAT GTTGCGGCTC GCTATGGTAA TGTGGATGTG 
GTCCAATACC TTTGCAGCAT CCATGCCAAC CCTGACCTGT TGGACCGGGA GCAAGAGACG 
CCTCTCCATT GCGCGGCCTG GCACGGTTAT TCGGCAGTGG CCAGAGCCCT GTGTGAGGCT 
GGCTGTGATG TTAATGCCCG TAACCGTGAG GGTGAGAGTC CACTGCTAAC CGCTTCAGCC 
CGTGGCTTCA AGGACATTGT GGAGTGCTTG CTGGAGCATG GGGCTGACAT GGACTCTGCT 
GATAAGGATG GTCACATCGC TCTGCATCTA GCCGTGAGGA GGTGCCAGGT TGAGGTGGTC 
AAGTGTTTGC TCAGCCATCA CTGCTATGTG GACCACCAGG ATCGTCACGG CAACACACCC 
CTTCACATCG CCTGTAAAGA CGCCAACATG CCTGTGGTCA CGCAGCTCTG TGCTGCCAAA 
GCCAACCTGG ATATTCCCAA CAAATTTGGG AGAACACCTC TTCACCTGGC AGCGAGTAAC 
GGGGGTCTGG AGGTGGTGCG GCATCTCTGT CTTTCAGGAG CAAACACTGA GGCCATCACT 
AATGATGGAA AGACGGCGGA GGATCTGGCC ACTGCAGAGC AGCAGGAGCA TGTAGCAGTG 
CTGCTGGCCA AGCTGAAAAA GGACAACCAT AAGAGCATTT ACATCCAGCA GCTGCGGCCT 
ACACAGACCC TCCAACATCG CCTCAAACTC AAGCTGTTCG GCCATTCTGG AGCAGGGAAG 
AGCACTCTGG TGGAGTCCTT GAAGTGTGGC ATCTTGAGGA GCTTCTTCAG AAGGAGGAGG 
ACCAGGCTGA CCAACCCTGT CCGACACCCA GCCTCGCCGG TCACTTCCAA ACCTGCAGTG 
TCTGTGAGCA TCTCTAACCT GTATCCGGGG TGTGAGAATG TGAGTGTTCG GAGCCGCAGT 
ATGATGTTCG AGCCCAGTTT GACTAAAGGA GTGCTGGAGG TCTTCAGTCC TGTTCATAAT 
GCTCTCTCCA CGGCTGACGA CACTGCCACT AAAGCCATCG ACATCCAGAA CGCCAACGTC 
AATGGTGTTG GAGACTTCAG TATATGGGAG TTTTCTGGCA ATCCCGTCTA TTATTGCTCG 
TATGACTACT TCGCTGCCAA TGATGCCACA GCGATTCATC TGGTCTTATT CAGCCTGGAG 
GAGCCCTACG AAACTCAGCT CAACCAGGTC ACATTCTGGC TGAACTGTCT AAAGGCTCTC 
ACGCTGCCAG AGGACAACAT CGCCTTTGGA GGGAAGATCA GGAATCCTCT GCGTGTGGTT 
CTGGTGGCCA CACATGCTGA CATTGTGAAT CTGCCCCGAT CGTTTGGAGG AGAGTTCAGC 
TATGACAAGG AGAGATCTCT ACTGAAGGAA GTCAGAAACA GGTTTGGAAA TGATCTGCTG 
ATCACTGAGA AGCTCTTTGT CATGGATGCA GGAGCGTCCA ACTCTAAAGA CATAAAGCTT 
CTGAGAAACC TTCTGTTTGA GCTCCGCAGC ACCATCATTT CTACCTGCAG GCCGATGACT 
CAGCTGGCAG AGAAGCTGTT GTCCACTCTG CCTTCCTGGA GGAAACTGAA CGGGCCGAAC 
CAGCTCATAT CATGGCAACA GTTTGTTGTG GATGTGCAAG AACAAATCAA CCCTCTAGCC 
AGCGAAGAGG ATCTGCGGGG ACTGGCTTCA CAACTTCACA GCATGGGAGA GATTAACATC 
ATGCAGAGTG AGACAGTCCA AGATGTTGTC CTCTTAGATC CTCGCTGGCT CTGTAGTAAC 
GTACTTGGCA AGCTTCTCTC AATTGAAACC CCCAAAGCAA TCCACCATTA TCGCGGCCGA 
TACCGTGTAG AAGAGCTCCA ACACCTGGTC TCCGAAGGCG ACATCGATGA ACTTCTCCAA 
GTCCTCGACG CCATGGACGT CTGCGCCCGC GACCTGGCCA ACCCAGGAAT GGTGGACATC 
CCTGCCCTCA TTCGCACAGG TGGCCTGCAG CATACCTGGA CAGACGAGGA GGACGTGGAG 
GGAGAGGACG CTCTGCTTTA CGGTGGAGTC CGTATGGTTC CTGTGGAGCA CCTGGCACCC 
TTTCCTTGCG GACTTTTCCA TAAGTTACAG GTGAACTTGT GCAGGTGGAG CCGCCAGCAG 
AAGCCTGAGG TGGAGGCTGA TGATGTGAGG TTGTGGAGCG GTGGCGCTCG CATCGGACTT 
GGTGGTGTGG AGGCTCTGGT TCTGCTTGTG AACCACGGGC AAGGCATGGA GGTACAGTTA 
CGAGGACCTG ACACAGATCC GGGACGCTGC TATGCTTTGT TGGACACTCT GTGCGGCATG 
GCTGAGGGTC TGTTAGCTGC TACTTTGCCC GGTTTGCTTA CCGCCCGACA CTATCTCAGC 
CCTCAGCAAC TGCGTGAACA CCACGGCCCA GTCATGCTTT ACCAGCCGAG GGACTTTTTT 
AGAGCTCAAG CGCGAGGTGA GAACGCTCTG TCGAACACCA TGGGTGGCTA TCGCGAAAGC 
TTTGTCAGTA TCGTAGCATT AGGATGCATT CATGTCTACA AACAGGGCAA CCTCGGTGGC 
GAAGTGGCTG TAGCAGAGGT CAGTCTTTTG GCTCGACGTA AGCTCTGCCG CCTGCTAGAC 
CCGCCTGATG CCCTGGGCAA GGACTGGTGC CTGTTGGCCA TGAACTTGGG CCTTAGCGAA 
CTAGCTGCTA AATACAGTTC TGGCATCAAC GGGACTCCAC CAGCAGACCC GCTTTCTCTC 
CCTAGCCCTA CAGCCACACT CCTGCAGGAG TGGAGCCAGA GGCCTGACTC AACCATTGGT 
GTTTTGGTTG CAAAGTTGCG AGAACTTGGC CGTCGAGACG CGGCAGATTT CCTGCTTAGA 
GCTGCACCAG TGTTTCGGGT GAACTTGGAG ATTTTGGGCA CCCCGTCTGA GCCCTACAGC 
CCCGTGTGTA ATGGAGGAGG CACATCCTAC AACTCCATCA GCTCCGTCAT ATCTCGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dapk1 ( XM_017356100.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017211589.1 Danio rerio death-associated protein kinase 1 (dapk1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa10713
Clone ID Related Accession (Same CDS sequence)	NM_001099990.2 , XM_017356101.1 , XM_017356100.2 , XM_017356100.1 , XM_017356101.2
Accession Version	XM_017356100.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4320bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	death-associated protein kinase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017356100.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGTGT TCAACCAGGA GAATGTGGAG GATTATTATG AGATTGGAGG AGAACTCGGG 
AGTGGTCAGT TTGCCGTGGT AAAGAAATGC CGTCATAAGA GCACTGGTGT GGAGTATGCA 
GCCAAATTTA TCAAGAAGAG GCGGAGCAAA TCAAGCCGAC GAGGGGTCTC AAAAGACGAC 
ATTGAGAGGG AGGTCAGCAT CCTGAAGGAA ATCCAGCACC CCAATGTCAT CACCCTGCAC 
GATGTGTTCG AAAACAAGCA TGAAGTCATC CTCATCCTAG AGCTTGTGGC TGGCGGAGAA 
CTCTTTGACT TTCTAGCAGA GAAAGAGTCA CTGAGTGAAG AGGAGGCCAC CGAGTTCCTC 
AAGCAGATCT TGGATGGAGT CAGTTACCTG CACTCGAAAC GGATTTCCCA CTTTGACCTG 
AAGCCGGAGA ACATCATGCT GTTAAACCGG AACGTGCCTC ACCCGCGCAT TAAGCTCATT 
GACTTTGGCC TGGCCCACAA GATCGATTTT GGCAACGATT TCAAAAACAT CTTTGGTACC 
CCGGAGTTTG TAGCTCCAGA AGTGGTGAAC TATGAGCCTC TTGGCCTGGA GGCAGACATG 
TGGAGCATTG GAGTAATCAC TTATATTCTG CTTAGCGGTG CTTCACCTTT CCTGGGTGAA 
AACAAGCAGG AGACGCTTGC CAATGTGTCA GCCGTGGACT ATGAGTTTGA TGAGGACTAT 
TTCAGCCACA CCAGCGCTTT GGCCAAAGAC TTCATTGCAC GGTTACTGAT CAAAGATCCA 
AAAAAAAGAA TGACAATCCT GGACAGTCTT CAACATCCCT GGATCAAGCC AAAGGATACA 
CAGCAGGCAC TGAGTCGCAA AGAATCAGCT GTCAATATGG AGAAATTCAA GAAGTTTGCT 
GCCCGAAGAA AATGGAAGCA ATCAGTGCGT CTCATCTCTC TGTGTAACCG GCTCTCCCGC 
TCCTTTCTGT CCAGGAGCAA CATGAGTGTG GCACGCAGTG ACGACACATT GGATGAAGAG 
GACTCGTTTG TCATGAAGGC TATCATCCAC GCAATAAACG ACGACAATGT TCCCGGTCTG 
AAACACCTAC TTGGATCTCT GACCAGCTAT GATATCAATC AACCTAACAA GCATGGAACT 
CCTCCGCTCC TGATCGCTGC AGGCTGTGGA AACGTCCAGA TCATTGATGT GCTCATGAAA 
AAAGGGGCTG AAATTCAAGC CTTTGATAAG ACTGGAGCCA GTGCTATCTA CTACGCCGCC 
CGACATGGTC ATGTGGGGAC CTTGAGATTC TTGCATGAAA AGAAGTGCCC TCTGGACATC 
CAAGATAAGT CCGGAGAAAC TGCTCTGCAT GTTGCGGCTC GCTATGGTAA TGTGGATGTG 
GTCCAATACC TTTGCAGCAT CCATGCCAAC CCTGACCTGT TGGACCGGGA GCAAGAGACG 
CCTCTCCATT GCGCGGCCTG GCACGGTTAT TCGGCAGTGG CCAGAGCCCT GTGTGAGGCT 
GGCTGTGATG TTAATGCCCG TAACCGTGAG GGTGAGAGTC CACTGCTAAC CGCTTCAGCC 
CGTGGCTTCA AGGACATTGT GGAGTGCTTG CTGGAGCATG GGGCTGACAT GGACTCTGCT 
GATAAGGATG GTCACATCGC TCTGCATCTA GCCGTGAGGA GGTGCCAGGT TGAGGTGGTC 
AAGTGTTTGC TCAGCCATCA CTGCTATGTG GACCACCAGG ATCGTCACGG CAACACACCC 
CTTCACATCG CCTGTAAAGA CGCCAACATG CCTGTGGTCA CGCAGCTCTG TGCTGCCAAA 
GCCAACCTGG ATATTCCCAA CAAATTTGGG AGAACACCTC TTCACCTGGC AGCGAGTAAC 
GGGGGTCTGG AGGTGGTGCG GCATCTCTGT CTTTCAGGAG CAAACACTGA GGCCATCACT 
AATGATGGAA AGACGGCGGA GGATCTGGCC ACTGCAGAGC AGCAGGAGCA TGTAGCAGTG 
CTGCTGGCCA AGCTGAAAAA GGACAACCAT AAGAGCATTT ACATCCAGCA GCTGCGGCCT 
ACACAGACCC TCCAACATCG CCTCAAACTC AAGCTGTTCG GCCATTCTGG AGCAGGGAAG 
AGCACTCTGG TGGAGTCCTT GAAGTGTGGC ATCTTGAGGA GCTTCTTCAG AAGGAGGAGG 
ACCAGGCTGA CCAACCCTGT CCGACACCCA GCCTCGCCGG TCACTTCCAA ACCTGCAGTG 
TCTGTGAGCA TCTCTAACCT GTATCCGGGG TGTGAGAATG TGAGTGTTCG GAGCCGCAGT 
ATGATGTTCG AGCCCAGTTT GACTAAAGGA GTGCTGGAGG TCTTCAGTCC TGTTCATAAT 
GCTCTCTCCA CGGCTGACGA CACTGCCACT AAAGCCATCG ACATCCAGAA CGCCAACGTC 
AATGGTGTTG GAGACTTCAG TATATGGGAG TTTTCTGGCA ATCCCGTCTA TTATTGCTCG 
TATGACTACT TCGCTGCCAA TGATGCCACA GCGATTCATC TGGTCTTATT CAGCCTGGAG 
GAGCCCTACG AAACTCAGCT CAACCAGGTC ACATTCTGGC TGAACTGTCT AAAGGCTCTC 
ACGCTGCCAG AGGACAACAT CGCCTTTGGA GGGAAGATCA GGAATCCTCT GCGTGTGGTT 
CTGGTGGCCA CACATGCTGA CATTGTGAAT CTGCCCCGAT CGTTTGGAGG AGAGTTCAGC 
TATGACAAGG AGAGATCTCT ACTGAAGGAA GTCAGAAACA GGTTTGGAAA TGATCTGCTG 
ATCACTGAGA AGCTCTTTGT CATGGATGCA GGAGCGTCCA ACTCTAAAGA CATAAAGCTT 
CTGAGAAACC TTCTGTTTGA GCTCCGCAGC ACCATCATTT CTACCTGCAG GCCGATGACT 
CAGCTGGCAG AGAAGCTGTT GTCCACTCTG CCTTCCTGGA GGAAACTGAA CGGGCCGAAC 
CAGCTCATAT CATGGCAACA GTTTGTTGTG GATGTGCAAG AACAAATCAA CCCTCTAGCC 
AGCGAAGAGG ATCTGCGGGG ACTGGCTTCA CAACTTCACA GCATGGGAGA GATTAACATC 
ATGCAGAGTG AGACAGTCCA AGATGTTGTC CTCTTAGATC CTCGCTGGCT CTGTAGTAAC 
GTACTTGGCA AGCTTCTCTC AATTGAAACC CCCAAAGCAA TCCACCATTA TCGCGGCCGA 
TACCGTGTAG AAGAGCTCCA ACACCTGGTC TCCGAAGGCG ACATCGATGA ACTTCTCCAA 
GTCCTCGACG CCATGGACGT CTGCGCCCGC GACCTGGCCA ACCCAGGAAT GGTGGACATC 
CCTGCCCTCA TTCGCACAGG TGGCCTGCAG CATACCTGGA CAGACGAGGA GGACGTGGAG 
GGAGAGGACG CTCTGCTTTA CGGTGGAGTC CGTATGGTTC CTGTGGAGCA CCTGGCACCC 
TTTCCTTGCG GACTTTTCCA TAAGTTACAG GTGAACTTGT GCAGGTGGAG CCGCCAGCAG 
AAGCCTGAGG TGGAGGCTGA TGATGTGAGG TTGTGGAGCG GTGGCGCTCG CATCGGACTT 
GGTGGTGTGG AGGCTCTGGT TCTGCTTGTG AACCACGGGC AAGGCATGGA GGTACAGTTA 
CGAGGACCTG ACACAGATCC GGGACGCTGC TATGCTTTGT TGGACACTCT GTGCGGCATG 
GCTGAGGGTC TGTTAGCTGC TACTTTGCCC GGTTTGCTTA CCGCCCGACA CTATCTCAGC 
CCTCAGCAAC TGCGTGAACA CCACGGCCCA GTCATGCTTT ACCAGCCGAG GGACTTTTTT 
AGAGCTCAAG CGCGAGGTGA GAACGCTCTG TCGAACACCA TGGGTGGCTA TCGCGAAAGC 
TTTGTCAGTA TCGTAGCATT AGGATGCATT CATGTCTACA AACAGGGCAA CCTCGGTGGC 
GAAGTGGCTG TAGCAGAGGT CAGTCTTTTG GCTCGACGTA AGCTCTGCCG CCTGCTAGAC 
CCGCCTGATG CCCTGGGCAA GGACTGGTGC CTGTTGGCCA TGAACTTGGG CCTTAGCGAA 
CTAGCTGCTA AATACAGTTC TGGCATCAAC GGGACTCCAC CAGCAGACCC GCTTTCTCTC 
CCTAGCCCTA CAGCCACACT CCTGCAGGAG TGGAGCCAGA GGCCTGACTC AACCATTGGT 
GTTTTGGTTG CAAAGTTGCG AGAACTTGGC CGTCGAGACG CGGCAGATTT CCTGCTTAGA 
GCTGCACCAG TGTTTCGGGT GAACTTGGAG ATTTTGGGCA CCCCGTCTGA GCCCTACAGC 
CCCGTGTGTA ATGGAGGAGG CACATCCTAC AACTCCATCA GCTCCGTCAT ATCTCGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017211589.1
CDS	248..4567
Translation	MTVFNQENVEDYYEIGGELGSGQFAVVKKCRHKSTGVEYAAKFIKKRRSKSSRRGVSKDDIEREVSILKEIQHPNVITLHDVFENKHEVILILELVAGGELFDFLAEKESLSEEEATEFLKQILDGVSYLHSKRISHFDLKPENIMLLNRNVPHPRIKLIDFGLAHKIDFGNDFKNIFGTPEFVAPEVVNYEPLGLEADMWSIGVITYILLSGASPFLGENKQETLANVSAVDYEFDEDYFSHTSALAKDFIARLLIKDPKKRMTILDSLQHPWIKPKDTQQALSRKESAVNMEKFKKFAARRKWKQSVRLISLCNRLSRSFLSRSNMSVARSDDTLDEEDSFVMKAIIHAINDDNVPGLKHLLGSLTSYDINQPNKHGTPPLLIAAGCGNVQIIDVLMKKGAEIQAFDKTGASAIYYAARHGHVGTLRFLHEKKCPLDIQDKSGETALHVAARYGNVDVVQYLCSIHANPDLLDREQETPLHCAAWHGYSAVARALCEAGCDVNARNREGESPLLTASARGFKDIVECLLEHGADMDSADKDGHIALHLAVRRCQVEVVKCLLSHHCYVDHQDRHGNTPLHIACKDANMPVVTQLCAAKANLDIPNKFGRTPLHLAASNGGLEVVRHLCLSGANTEAITNDGKTAEDLATAEQQEHVAVLLAKLKKDNHKSIYIQQLRPTQTLQHRLKLKLFGHSGAGKSTLVESLKCGILRSFFRRRRTRLTNPVRHPASPVTSKPAVSVSISNLYPGCENVSVRSRSMMFEPSLTKGVLEVFSPVHNALSTADDTATKAIDIQNANVNGVGDFSIWEFSGNPVYYCSYDYFAANDATAIHLVLFSLEEPYETQLNQVTFWLNCLKALTLPEDNIAFGGKIRNPLRVVLVATHADIVNLPRSFGGEFSYDKERSLLKEVRNRFGNDLLITEKLFVMDAGASNSKDIKLLRNLLFELRSTIISTCRPMTQLAEKLLSTLPSWRKLNGPNQLISWQQFVVDVQEQINPLASEEDLRGLASQLHSMGEINIMQSETVQDVVLLDPRWLCSNVLGKLLSIETPKAIHHYRGRYRVEELQHLVSEGDIDELLQVLDAMDVCARDLANPGMVDIPALIRTGGLQHTWTDEEDVEGEDALLYGGVRMVPVEHLAPFPCGLFHKLQVNLCRWSRQQKPEVEADDVRLWSGGARIGLGGVEALVLLVNHGQGMEVQLRGPDTDPGRCYALLDTLCGMAEGLLAATLPGLLTARHYLSPQQLREHHGPVMLYQPRDFFRAQARGENALSNTMGGYRESFVSIVALGCIHVYKQGNLGGEVAVAEVSLLARRKLCRLLDPPDALGKDWCLLAMNLGLSELAAKYSSGINGTPPADPLSLPSPTATLLQEWSQRPDSTIGVLVAKLRELGRRDAADFLLRAAPVFRVNLEILGTPSEPYSPVCNGGGTSYNSISSVISR

Target ORF information:

RefSeq Version	XM_017356100.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio death-associated protein kinase 1 (dapk1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356100.2

ORF Insert Sequence:

ATGACCGTGT TCAACCAGGA GAATGTGGAG GATTATTATG AGATTGGAGG AGAACTCGGG 
AGTGGTCAGT TTGCCGTGGT AAAGAAATGC CGTCATAAGA GCACTGGTGT GGAGTATGCA 
GCCAAATTTA TCAAGAAGAG GCGGAGCAAA TCAAGCCGAC GAGGGGTCTC AAAAGACGAC 
ATTGAGAGGG AGGTCAGCAT CCTGAAGGAA ATCCAGCACC CCAATGTCAT CACCCTGCAC 
GATGTGTTCG AAAACAAGCA TGAAGTCATC CTCATCCTAG AGCTTGTGGC TGGCGGAGAA 
CTCTTTGACT TTCTAGCAGA GAAAGAGTCA CTGAGTGAAG AGGAGGCCAC CGAGTTCCTC 
AAGCAGATCT TGGATGGAGT CAGTTACCTG CACTCGAAAC GGATTTCCCA CTTTGACCTG 
AAGCCGGAGA ACATCATGCT GTTAAACCGG AACGTGCCTC ACCCGCGCAT TAAGCTCATT 
GACTTTGGCC TGGCCCACAA GATCGATTTT GGCAACGATT TCAAAAACAT CTTTGGTACC 
CCGGAGTTTG TAGCTCCAGA AGTGGTGAAC TATGAGCCTC TTGGCCTGGA GGCAGACATG 
TGGAGCATTG GAGTAATCAC TTATATTCTG CTTAGCGGTG CTTCACCTTT CCTGGGTGAA 
AACAAGCAGG AGACGCTTGC CAATGTGTCA GCCGTGGACT ATGAGTTTGA TGAGGACTAT 
TTCAGCCACA CCAGCGCTTT GGCCAAAGAC TTCATTGCAC GGTTACTGAT CAAAGATCCA 
AAAAAAAGAA TGACAATCCT GGACAGTCTT CAACATCCCT GGATCAAGCC AAAGGATACA 
CAGCAGGCAC TGAGTCGCAA AGAATCAGCT GTCAATATGG AGAAATTCAA GAAGTTTGCT 
GCCCGAAGAA AATGGAAGCA ATCAGTGCGT CTCATCTCTC TGTGTAACCG GCTCTCCCGC 
TCCTTTCTGT CCAGGAGCAA CATGAGTGTG GCACGCAGTG ACGACACATT GGATGAAGAG 
GACTCGTTTG TCATGAAGGC TATCATCCAC GCAATAAACG ACGACAATGT TCCCGGTCTG 
AAACACCTAC TTGGATCTCT GACCAGCTAT GATATCAATC AACCTAACAA GCATGGAACT 
CCTCCGCTCC TGATCGCTGC AGGCTGTGGA AACGTCCAGA TCATTGATGT GCTCATGAAA 
AAAGGGGCTG AAATTCAAGC CTTTGATAAG ACTGGAGCCA GTGCTATCTA CTACGCCGCC 
CGACATGGTC ATGTGGGGAC CTTGAGATTC TTGCATGAAA AGAAGTGCCC TCTGGACATC 
CAAGATAAGT CCGGAGAAAC TGCTCTGCAT GTTGCGGCTC GCTATGGTAA TGTGGATGTG 
GTCCAATACC TTTGCAGCAT CCATGCCAAC CCTGACCTGT TGGACCGGGA GCAAGAGACG 
CCTCTCCATT GCGCGGCCTG GCACGGTTAT TCGGCAGTGG CCAGAGCCCT GTGTGAGGCT 
GGCTGTGATG TTAATGCCCG TAACCGTGAG GGTGAGAGTC CACTGCTAAC CGCTTCAGCC 
CGTGGCTTCA AGGACATTGT GGAGTGCTTG CTGGAGCATG GGGCTGACAT GGACTCTGCT 
GATAAGGATG GTCACATCGC TCTGCATCTA GCCGTGAGGA GGTGCCAGGT TGAGGTGGTC 
AAGTGTTTGC TCAGCCATCA CTGCTATGTG GACCACCAGG ATCGTCACGG CAACACACCC 
CTTCACATCG CCTGTAAAGA CGCCAACATG CCTGTGGTCA CGCAGCTCTG TGCTGCCAAA 
GCCAACCTGG ATATTCCCAA CAAATTTGGG AGAACACCTC TTCACCTGGC AGCGAGTAAC 
GGGGGTCTGG AGGTGGTGCG GCATCTCTGT CTTTCAGGAG CAAACACTGA GGCCATCACT 
AATGATGGAA AGACGGCGGA GGATCTGGCC ACTGCAGAGC AGCAGGAGCA TGTAGCAGTG 
CTGCTGGCCA AGCTGAAAAA GGACAACCAT AAGAGCATTT ACATCCAGCA GCTGCGGCCT 
ACACAGACCC TCCAACATCG CCTCAAACTC AAGCTGTTCG GCCATTCTGG AGCAGGGAAG 
AGCACTCTGG TGGAGTCCTT GAAGTGTGGC ATCTTGAGGA GCTTCTTCAG AAGGAGGAGG 
ACCAGGCTGA CCAACCCTGT CCGACACCCA GCCTCGCCGG TCACTTCCAA ACCTGCAGTG 
TCTGTGAGCA TCTCTAACCT GTATCCGGGG TGTGAGAATG TGAGTGTTCG GAGCCGCAGT 
ATGATGTTCG AGCCCAGTTT GACTAAAGGA GTGCTGGAGG TCTTCAGTCC TGTTCATAAT 
GCTCTCTCCA CGGCTGACGA CACTGCCACT AAAGCCATCG ACATCCAGAA CGCCAACGTC 
AATGGTGTTG GAGACTTCAG TATATGGGAG TTTTCTGGCA ATCCCGTCTA TTATTGCTCG 
TATGACTACT TCGCTGCCAA TGATGCCACA GCGATTCATC TGGTCTTATT CAGCCTGGAG 
GAGCCCTACG AAACTCAGCT CAACCAGGTC ACATTCTGGC TGAACTGTCT AAAGGCTCTC 
ACGCTGCCAG AGGACAACAT CGCCTTTGGA GGGAAGATCA GGAATCCTCT GCGTGTGGTT 
CTGGTGGCCA CACATGCTGA CATTGTGAAT CTGCCCCGAT CGTTTGGAGG AGAGTTCAGC 
TATGACAAGG AGAGATCTCT ACTGAAGGAA GTCAGAAACA GGTTTGGAAA TGATCTGCTG 
ATCACTGAGA AGCTCTTTGT CATGGATGCA GGAGCGTCCA ACTCTAAAGA CATAAAGCTT 
CTGAGAAACC TTCTGTTTGA GCTCCGCAGC ACCATCATTT CTACCTGCAG GCCGATGACT 
CAGCTGGCAG AGAAGCTGTT GTCCACTCTG CCTTCCTGGA GGAAACTGAA CGGGCCGAAC 
CAGCTCATAT CATGGCAACA GTTTGTTGTG GATGTGCAAG AACAAATCAA CCCTCTAGCC 
AGCGAAGAGG ATCTGCGGGG ACTGGCTTCA CAACTTCACA GCATGGGAGA GATTAACATC 
ATGCAGAGTG AGACAGTCCA AGATGTTGTC CTCTTAGATC CTCGCTGGCT CTGTAGTAAC 
GTACTTGGCA AGCTTCTCTC AATTGAAACC CCCAAAGCAA TCCACCATTA TCGCGGCCGA 
TACCGTGTAG AAGAGCTCCA ACACCTGGTC TCCGAAGGCG ACATCGATGA ACTTCTCCAA 
GTCCTCGACG CCATGGACGT CTGCGCCCGC GACCTGGCCA ACCCAGGAAT GGTGGACATC 
CCTGCCCTCA TTCGCACAGG TGGCCTGCAG CATACCTGGA CAGACGAGGA GGACGTGGAG 
GGAGAGGACG CTCTGCTTTA CGGTGGAGTC CGTATGGTTC CTGTGGAGCA CCTGGCACCC 
TTTCCTTGCG GACTTTTCCA TAAGTTACAG GTGAACTTGT GCAGGTGGAG CCGCCAGCAG 
AAGCCTGAGG TGGAGGCTGA TGATGTGAGG TTGTGGAGCG GTGGCGCTCG CATCGGACTT 
GGTGGTGTGG AGGCTCTGGT TCTGCTTGTG AACCACGGGC AAGGCATGGA GGTACAGTTA 
CGAGGACCTG ACACAGATCC GGGACGCTGC TATGCTTTGT TGGACACTCT GTGCGGCATG 
GCTGAGGGTC TGTTAGCTGC TACTTTGCCC GGTTTGCTTA CCGCCCGACA CTATCTCAGC 
CCTCAGCAAC TGCGTGAACA CCACGGCCCA GTCATGCTTT ACCAGCCGAG GGACTTTTTT 
AGAGCTCAAG CGCGAGGTGA GAACGCTCTG TCGAACACCA TGGGTGGCTA TCGCGAAAGC 
TTTGTCAGTA TCGTAGCATT AGGATGCATT CATGTCTACA AACAGGGCAA CCTCGGTGGC 
GAAGTGGCTG TAGCAGAGGT CAGTCTTTTG GCTCGACGTA AGCTCTGCCG CCTGCTAGAC 
CCGCCTGATG CCCTGGGCAA GGACTGGTGC CTGTTGGCCA TGAACTTGGG CCTTAGCGAA 
CTAGCTGCTA AATACAGTTC TGGCATCAAC GGGACTCCAC CAGCAGACCC GCTTTCTCTC 
CCTAGCCCTA CAGCCACACT CCTGCAGGAG TGGAGCCAGA GGCCTGACTC AACCATTGGT 
GTTTTGGTTG CAAAGTTGCG AGAACTTGGC CGTCGAGACG CGGCAGATTT CCTGCTTAGA 
GCTGCACCAG TGTTTCGGGT GAACTTGGAG ATTTTGGGCA CCCCGTCTGA GCCCTACAGC 
CCCGTGTGTA ATGGAGGAGG CACATCCTAC AACTCCATCA GCTCCGTCAT ATCTCGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dapk1 ( XM_017356100.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017211589.1 Danio rerio death-associated protein kinase 1 (dapk1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa10713
Clone ID Related Accession (Same CDS sequence)	NM_001099990.2 , XM_017356101.1 , XM_017356100.2 , XM_017356100.1 , XM_017356101.2
Accession Version	XM_017356100.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4320bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	death-associated protein kinase 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGTGT TCAACCAGGA GAATGTGGAG GATTATTATG AGATTGGAGG AGAACTCGGG 
AGTGGTCAGT TTGCCGTGGT AAAGAAATGC CGTCATAAGA GCACTGGTGT GGAGTATGCA 
GCCAAATTTA TCAAGAAGAG GCGGAGCAAA TCAAGCCGAC GAGGGGTCTC AAAAGACGAC 
ATTGAGAGGG AGGTCAGCAT CCTGAAGGAA ATCCAGCACC CCAATGTCAT CACCCTGCAC 
GATGTGTTCG AAAACAAGCA TGAAGTCATC CTCATCCTAG AGCTTGTGGC TGGCGGAGAA 
CTCTTTGACT TTCTAGCAGA GAAAGAGTCA CTGAGTGAAG AGGAGGCCAC CGAGTTCCTC 
AAGCAGATCT TGGATGGAGT CAGTTACCTG CACTCGAAAC GGATTTCCCA CTTTGACCTG 
AAGCCGGAGA ACATCATGCT GTTAAACCGG AACGTGCCTC ACCCGCGCAT TAAGCTCATT 
GACTTTGGCC TGGCCCACAA GATCGATTTT GGCAACGATT TCAAAAACAT CTTTGGTACC 
CCGGAGTTTG TAGCTCCAGA AGTGGTGAAC TATGAGCCTC TTGGCCTGGA GGCAGACATG 
TGGAGCATTG GAGTAATCAC TTATATTCTG CTTAGCGGTG CTTCACCTTT CCTGGGTGAA 
AACAAGCAGG AGACGCTTGC CAATGTGTCA GCCGTGGACT ATGAGTTTGA TGAGGACTAT 
TTCAGCCACA CCAGCGCTTT GGCCAAAGAC TTCATTGCAC GGTTACTGAT CAAAGATCCA 
AAAAAAAGAA TGACAATCCT GGACAGTCTT CAACATCCCT GGATCAAGCC AAAGGATACA 
CAGCAGGCAC TGAGTCGCAA AGAATCAGCT GTCAATATGG AGAAATTCAA GAAGTTTGCT 
GCCCGAAGAA AATGGAAGCA ATCAGTGCGT CTCATCTCTC TGTGTAACCG GCTCTCCCGC 
TCCTTTCTGT CCAGGAGCAA CATGAGTGTG GCACGCAGTG ACGACACATT GGATGAAGAG 
GACTCGTTTG TCATGAAGGC TATCATCCAC GCAATAAACG ACGACAATGT TCCCGGTCTG 
AAACACCTAC TTGGATCTCT GACCAGCTAT GATATCAATC AACCTAACAA GCATGGAACT 
CCTCCGCTCC TGATCGCTGC AGGCTGTGGA AACGTCCAGA TCATTGATGT GCTCATGAAA 
AAAGGGGCTG AAATTCAAGC CTTTGATAAG ACTGGAGCCA GTGCTATCTA CTACGCCGCC 
CGACATGGTC ATGTGGGGAC CTTGAGATTC TTGCATGAAA AGAAGTGCCC TCTGGACATC 
CAAGATAAGT CCGGAGAAAC TGCTCTGCAT GTTGCGGCTC GCTATGGTAA TGTGGATGTG 
GTCCAATACC TTTGCAGCAT CCATGCCAAC CCTGACCTGT TGGACCGGGA GCAAGAGACG 
CCTCTCCATT GCGCGGCCTG GCACGGTTAT TCGGCAGTGG CCAGAGCCCT GTGTGAGGCT 
GGCTGTGATG TTAATGCCCG TAACCGTGAG GGTGAGAGTC CACTGCTAAC CGCTTCAGCC 
CGTGGCTTCA AGGACATTGT GGAGTGCTTG CTGGAGCATG GGGCTGACAT GGACTCTGCT 
GATAAGGATG GTCACATCGC TCTGCATCTA GCCGTGAGGA GGTGCCAGGT TGAGGTGGTC 
AAGTGTTTGC TCAGCCATCA CTGCTATGTG GACCACCAGG ATCGTCACGG CAACACACCC 
CTTCACATCG CCTGTAAAGA CGCCAACATG CCTGTGGTCA CGCAGCTCTG TGCTGCCAAA 
GCCAACCTGG ATATTCCCAA CAAATTTGGG AGAACACCTC TTCACCTGGC AGCGAGTAAC 
GGGGGTCTGG AGGTGGTGCG GCATCTCTGT CTTTCAGGAG CAAACACTGA GGCCATCACT 
AATGATGGAA AGACGGCGGA GGATCTGGCC ACTGCAGAGC AGCAGGAGCA TGTAGCAGTG 
CTGCTGGCCA AGCTGAAAAA GGACAACCAT AAGAGCATTT ACATCCAGCA GCTGCGGCCT 
ACACAGACCC TCCAACATCG CCTCAAACTC AAGCTGTTCG GCCATTCTGG AGCAGGGAAG 
AGCACTCTGG TGGAGTCCTT GAAGTGTGGC ATCTTGAGGA GCTTCTTCAG AAGGAGGAGG 
ACCAGGCTGA CCAACCCTGT CCGACACCCA GCCTCGCCGG TCACTTCCAA ACCTGCAGTG 
TCTGTGAGCA TCTCTAACCT GTATCCGGGG TGTGAGAATG TGAGTGTTCG GAGCCGCAGT 
ATGATGTTCG AGCCCAGTTT GACTAAAGGA GTGCTGGAGG TCTTCAGTCC TGTTCATAAT 
GCTCTCTCCA CGGCTGACGA CACTGCCACT AAAGCCATCG ACATCCAGAA CGCCAACGTC 
AATGGTGTTG GAGACTTCAG TATATGGGAG TTTTCTGGCA ATCCCGTCTA TTATTGCTCG 
TATGACTACT TCGCTGCCAA TGATGCCACA GCGATTCATC TGGTCTTATT CAGCCTGGAG 
GAGCCCTACG AAACTCAGCT CAACCAGGTC ACATTCTGGC TGAACTGTCT AAAGGCTCTC 
ACGCTGCCAG AGGACAACAT CGCCTTTGGA GGGAAGATCA GGAATCCTCT GCGTGTGGTT 
CTGGTGGCCA CACATGCTGA CATTGTGAAT CTGCCCCGAT CGTTTGGAGG AGAGTTCAGC 
TATGACAAGG AGAGATCTCT ACTGAAGGAA GTCAGAAACA GGTTTGGAAA TGATCTGCTG 
ATCACTGAGA AGCTCTTTGT CATGGATGCA GGAGCGTCCA ACTCTAAAGA CATAAAGCTT 
CTGAGAAACC TTCTGTTTGA GCTCCGCAGC ACCATCATTT CTACCTGCAG GCCGATGACT 
CAGCTGGCAG AGAAGCTGTT GTCCACTCTG CCTTCCTGGA GGAAACTGAA CGGGCCGAAC 
CAGCTCATAT CATGGCAACA GTTTGTTGTG GATGTGCAAG AACAAATCAA CCCTCTAGCC 
AGCGAAGAGG ATCTGCGGGG ACTGGCTTCA CAACTTCACA GCATGGGAGA GATTAACATC 
ATGCAGAGTG AGACAGTCCA AGATGTTGTC CTCTTAGATC CTCGCTGGCT CTGTAGTAAC 
GTACTTGGCA AGCTTCTCTC AATTGAAACC CCCAAAGCAA TCCACCATTA TCGCGGCCGA 
TACCGTGTAG AAGAGCTCCA ACACCTGGTC TCCGAAGGCG ACATCGATGA ACTTCTCCAA 
GTCCTCGACG CCATGGACGT CTGCGCCCGC GACCTGGCCA ACCCAGGAAT GGTGGACATC 
CCTGCCCTCA TTCGCACAGG TGGCCTGCAG CATACCTGGA CAGACGAGGA GGACGTGGAG 
GGAGAGGACG CTCTGCTTTA CGGTGGAGTC CGTATGGTTC CTGTGGAGCA CCTGGCACCC 
TTTCCTTGCG GACTTTTCCA TAAGTTACAG GTGAACTTGT GCAGGTGGAG CCGCCAGCAG 
AAGCCTGAGG TGGAGGCTGA TGATGTGAGG TTGTGGAGCG GTGGCGCTCG CATCGGACTT 
GGTGGTGTGG AGGCTCTGGT TCTGCTTGTG AACCACGGGC AAGGCATGGA GGTACAGTTA 
CGAGGACCTG ACACAGATCC GGGACGCTGC TATGCTTTGT TGGACACTCT GTGCGGCATG 
GCTGAGGGTC TGTTAGCTGC TACTTTGCCC GGTTTGCTTA CCGCCCGACA CTATCTCAGC 
CCTCAGCAAC TGCGTGAACA CCACGGCCCA GTCATGCTTT ACCAGCCGAG GGACTTTTTT 
AGAGCTCAAG CGCGAGGTGA GAACGCTCTG TCGAACACCA TGGGTGGCTA TCGCGAAAGC 
TTTGTCAGTA TCGTAGCATT AGGATGCATT CATGTCTACA AACAGGGCAA CCTCGGTGGC 
GAAGTGGCTG TAGCAGAGGT CAGTCTTTTG GCTCGACGTA AGCTCTGCCG CCTGCTAGAC 
CCGCCTGATG CCCTGGGCAA GGACTGGTGC CTGTTGGCCA TGAACTTGGG CCTTAGCGAA 
CTAGCTGCTA AATACAGTTC TGGCATCAAC GGGACTCCAC CAGCAGACCC GCTTTCTCTC 
CCTAGCCCTA CAGCCACACT CCTGCAGGAG TGGAGCCAGA GGCCTGACTC AACCATTGGT 
GTTTTGGTTG CAAAGTTGCG AGAACTTGGC CGTCGAGACG CGGCAGATTT CCTGCTTAGA 
GCTGCACCAG TGTTTCGGGT GAACTTGGAG ATTTTGGGCA CCCCGTCTGA GCCCTACAGC 
CCCGTGTGTA ATGGAGGAGG CACATCCTAC AACTCCATCA GCTCCGTCAT ATCTCGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017211589.1
CDS	264..4583
Translation	MTVFNQENVEDYYEIGGELGSGQFAVVKKCRHKSTGVEYAAKFIKKRRSKSSRRGVSKDDIEREVSILKEIQHPNVITLHDVFENKHEVILILELVAGGELFDFLAEKESLSEEEATEFLKQILDGVSYLHSKRISHFDLKPENIMLLNRNVPHPRIKLIDFGLAHKIDFGNDFKNIFGTPEFVAPEVVNYEPLGLEADMWSIGVITYILLSGASPFLGENKQETLANVSAVDYEFDEDYFSHTSALAKDFIARLLIKDPKKRMTILDSLQHPWIKPKDTQQALSRKESAVNMEKFKKFAARRKWKQSVRLISLCNRLSRSFLSRSNMSVARSDDTLDEEDSFVMKAIIHAINDDNVPGLKHLLGSLTSYDINQPNKHGTPPLLIAAGCGNVQIIDVLMKKGAEIQAFDKTGASAIYYAARHGHVGTLRFLHEKKCPLDIQDKSGETALHVAARYGNVDVVQYLCSIHANPDLLDREQETPLHCAAWHGYSAVARALCEAGCDVNARNREGESPLLTASARGFKDIVECLLEHGADMDSADKDGHIALHLAVRRCQVEVVKCLLSHHCYVDHQDRHGNTPLHIACKDANMPVVTQLCAAKANLDIPNKFGRTPLHLAASNGGLEVVRHLCLSGANTEAITNDGKTAEDLATAEQQEHVAVLLAKLKKDNHKSIYIQQLRPTQTLQHRLKLKLFGHSGAGKSTLVESLKCGILRSFFRRRRTRLTNPVRHPASPVTSKPAVSVSISNLYPGCENVSVRSRSMMFEPSLTKGVLEVFSPVHNALSTADDTATKAIDIQNANVNGVGDFSIWEFSGNPVYYCSYDYFAANDATAIHLVLFSLEEPYETQLNQVTFWLNCLKALTLPEDNIAFGGKIRNPLRVVLVATHADIVNLPRSFGGEFSYDKERSLLKEVRNRFGNDLLITEKLFVMDAGASNSKDIKLLRNLLFELRSTIISTCRPMTQLAEKLLSTLPSWRKLNGPNQLISWQQFVVDVQEQINPLASEEDLRGLASQLHSMGEINIMQSETVQDVVLLDPRWLCSNVLGKLLSIETPKAIHHYRGRYRVEELQHLVSEGDIDELLQVLDAMDVCARDLANPGMVDIPALIRTGGLQHTWTDEEDVEGEDALLYGGVRMVPVEHLAPFPCGLFHKLQVNLCRWSRQQKPEVEADDVRLWSGGARIGLGGVEALVLLVNHGQGMEVQLRGPDTDPGRCYALLDTLCGMAEGLLAATLPGLLTARHYLSPQQLREHHGPVMLYQPRDFFRAQARGENALSNTMGGYRESFVSIVALGCIHVYKQGNLGGEVAVAEVSLLARRKLCRLLDPPDALGKDWCLLAMNLGLSELAAKYSSGINGTPPADPLSLPSPTATLLQEWSQRPDSTIGVLVAKLRELGRRDAADFLLRAAPVFRVNLEILGTPSEPYSPVCNGGGTSYNSISSVISR

Target ORF information:

RefSeq Version	XM_017356100.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio death-associated protein kinase 1 (dapk1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356100.1

ORF Insert Sequence:

ATGACCGTGT TCAACCAGGA GAATGTGGAG GATTATTATG AGATTGGAGG AGAACTCGGG 
AGTGGTCAGT TTGCCGTGGT AAAGAAATGC CGTCATAAGA GCACTGGTGT GGAGTATGCA 
GCCAAATTTA TCAAGAAGAG GCGGAGCAAA TCAAGCCGAC GAGGGGTCTC AAAAGACGAC 
ATTGAGAGGG AGGTCAGCAT CCTGAAGGAA ATCCAGCACC CCAATGTCAT CACCCTGCAC 
GATGTGTTCG AAAACAAGCA TGAAGTCATC CTCATCCTAG AGCTTGTGGC TGGCGGAGAA 
CTCTTTGACT TTCTAGCAGA GAAAGAGTCA CTGAGTGAAG AGGAGGCCAC CGAGTTCCTC 
AAGCAGATCT TGGATGGAGT CAGTTACCTG CACTCGAAAC GGATTTCCCA CTTTGACCTG 
AAGCCGGAGA ACATCATGCT GTTAAACCGG AACGTGCCTC ACCCGCGCAT TAAGCTCATT 
GACTTTGGCC TGGCCCACAA GATCGATTTT GGCAACGATT TCAAAAACAT CTTTGGTACC 
CCGGAGTTTG TAGCTCCAGA AGTGGTGAAC TATGAGCCTC TTGGCCTGGA GGCAGACATG 
TGGAGCATTG GAGTAATCAC TTATATTCTG CTTAGCGGTG CTTCACCTTT CCTGGGTGAA 
AACAAGCAGG AGACGCTTGC CAATGTGTCA GCCGTGGACT ATGAGTTTGA TGAGGACTAT 
TTCAGCCACA CCAGCGCTTT GGCCAAAGAC TTCATTGCAC GGTTACTGAT CAAAGATCCA 
AAAAAAAGAA TGACAATCCT GGACAGTCTT CAACATCCCT GGATCAAGCC AAAGGATACA 
CAGCAGGCAC TGAGTCGCAA AGAATCAGCT GTCAATATGG AGAAATTCAA GAAGTTTGCT 
GCCCGAAGAA AATGGAAGCA ATCAGTGCGT CTCATCTCTC TGTGTAACCG GCTCTCCCGC 
TCCTTTCTGT CCAGGAGCAA CATGAGTGTG GCACGCAGTG ACGACACATT GGATGAAGAG 
GACTCGTTTG TCATGAAGGC TATCATCCAC GCAATAAACG ACGACAATGT TCCCGGTCTG 
AAACACCTAC TTGGATCTCT GACCAGCTAT GATATCAATC AACCTAACAA GCATGGAACT 
CCTCCGCTCC TGATCGCTGC AGGCTGTGGA AACGTCCAGA TCATTGATGT GCTCATGAAA 
AAAGGGGCTG AAATTCAAGC CTTTGATAAG ACTGGAGCCA GTGCTATCTA CTACGCCGCC 
CGACATGGTC ATGTGGGGAC CTTGAGATTC TTGCATGAAA AGAAGTGCCC TCTGGACATC 
CAAGATAAGT CCGGAGAAAC TGCTCTGCAT GTTGCGGCTC GCTATGGTAA TGTGGATGTG 
GTCCAATACC TTTGCAGCAT CCATGCCAAC CCTGACCTGT TGGACCGGGA GCAAGAGACG 
CCTCTCCATT GCGCGGCCTG GCACGGTTAT TCGGCAGTGG CCAGAGCCCT GTGTGAGGCT 
GGCTGTGATG TTAATGCCCG TAACCGTGAG GGTGAGAGTC CACTGCTAAC CGCTTCAGCC 
CGTGGCTTCA AGGACATTGT GGAGTGCTTG CTGGAGCATG GGGCTGACAT GGACTCTGCT 
GATAAGGATG GTCACATCGC TCTGCATCTA GCCGTGAGGA GGTGCCAGGT TGAGGTGGTC 
AAGTGTTTGC TCAGCCATCA CTGCTATGTG GACCACCAGG ATCGTCACGG CAACACACCC 
CTTCACATCG CCTGTAAAGA CGCCAACATG CCTGTGGTCA CGCAGCTCTG TGCTGCCAAA 
GCCAACCTGG ATATTCCCAA CAAATTTGGG AGAACACCTC TTCACCTGGC AGCGAGTAAC 
GGGGGTCTGG AGGTGGTGCG GCATCTCTGT CTTTCAGGAG CAAACACTGA GGCCATCACT 
AATGATGGAA AGACGGCGGA GGATCTGGCC ACTGCAGAGC AGCAGGAGCA TGTAGCAGTG 
CTGCTGGCCA AGCTGAAAAA GGACAACCAT AAGAGCATTT ACATCCAGCA GCTGCGGCCT 
ACACAGACCC TCCAACATCG CCTCAAACTC AAGCTGTTCG GCCATTCTGG AGCAGGGAAG 
AGCACTCTGG TGGAGTCCTT GAAGTGTGGC ATCTTGAGGA GCTTCTTCAG AAGGAGGAGG 
ACCAGGCTGA CCAACCCTGT CCGACACCCA GCCTCGCCGG TCACTTCCAA ACCTGCAGTG 
TCTGTGAGCA TCTCTAACCT GTATCCGGGG TGTGAGAATG TGAGTGTTCG GAGCCGCAGT 
ATGATGTTCG AGCCCAGTTT GACTAAAGGA GTGCTGGAGG TCTTCAGTCC TGTTCATAAT 
GCTCTCTCCA CGGCTGACGA CACTGCCACT AAAGCCATCG ACATCCAGAA CGCCAACGTC 
AATGGTGTTG GAGACTTCAG TATATGGGAG TTTTCTGGCA ATCCCGTCTA TTATTGCTCG 
TATGACTACT TCGCTGCCAA TGATGCCACA GCGATTCATC TGGTCTTATT CAGCCTGGAG 
GAGCCCTACG AAACTCAGCT CAACCAGGTC ACATTCTGGC TGAACTGTCT AAAGGCTCTC 
ACGCTGCCAG AGGACAACAT CGCCTTTGGA GGGAAGATCA GGAATCCTCT GCGTGTGGTT 
CTGGTGGCCA CACATGCTGA CATTGTGAAT CTGCCCCGAT CGTTTGGAGG AGAGTTCAGC 
TATGACAAGG AGAGATCTCT ACTGAAGGAA GTCAGAAACA GGTTTGGAAA TGATCTGCTG 
ATCACTGAGA AGCTCTTTGT CATGGATGCA GGAGCGTCCA ACTCTAAAGA CATAAAGCTT 
CTGAGAAACC TTCTGTTTGA GCTCCGCAGC ACCATCATTT CTACCTGCAG GCCGATGACT 
CAGCTGGCAG AGAAGCTGTT GTCCACTCTG CCTTCCTGGA GGAAACTGAA CGGGCCGAAC 
CAGCTCATAT CATGGCAACA GTTTGTTGTG GATGTGCAAG AACAAATCAA CCCTCTAGCC 
AGCGAAGAGG ATCTGCGGGG ACTGGCTTCA CAACTTCACA GCATGGGAGA GATTAACATC 
ATGCAGAGTG AGACAGTCCA AGATGTTGTC CTCTTAGATC CTCGCTGGCT CTGTAGTAAC 
GTACTTGGCA AGCTTCTCTC AATTGAAACC CCCAAAGCAA TCCACCATTA TCGCGGCCGA 
TACCGTGTAG AAGAGCTCCA ACACCTGGTC TCCGAAGGCG ACATCGATGA ACTTCTCCAA 
GTCCTCGACG CCATGGACGT CTGCGCCCGC GACCTGGCCA ACCCAGGAAT GGTGGACATC 
CCTGCCCTCA TTCGCACAGG TGGCCTGCAG CATACCTGGA CAGACGAGGA GGACGTGGAG 
GGAGAGGACG CTCTGCTTTA CGGTGGAGTC CGTATGGTTC CTGTGGAGCA CCTGGCACCC 
TTTCCTTGCG GACTTTTCCA TAAGTTACAG GTGAACTTGT GCAGGTGGAG CCGCCAGCAG 
AAGCCTGAGG TGGAGGCTGA TGATGTGAGG TTGTGGAGCG GTGGCGCTCG CATCGGACTT 
GGTGGTGTGG AGGCTCTGGT TCTGCTTGTG AACCACGGGC AAGGCATGGA GGTACAGTTA 
CGAGGACCTG ACACAGATCC GGGACGCTGC TATGCTTTGT TGGACACTCT GTGCGGCATG 
GCTGAGGGTC TGTTAGCTGC TACTTTGCCC GGTTTGCTTA CCGCCCGACA CTATCTCAGC 
CCTCAGCAAC TGCGTGAACA CCACGGCCCA GTCATGCTTT ACCAGCCGAG GGACTTTTTT 
AGAGCTCAAG CGCGAGGTGA GAACGCTCTG TCGAACACCA TGGGTGGCTA TCGCGAAAGC 
TTTGTCAGTA TCGTAGCATT AGGATGCATT CATGTCTACA AACAGGGCAA CCTCGGTGGC 
GAAGTGGCTG TAGCAGAGGT CAGTCTTTTG GCTCGACGTA AGCTCTGCCG CCTGCTAGAC 
CCGCCTGATG CCCTGGGCAA GGACTGGTGC CTGTTGGCCA TGAACTTGGG CCTTAGCGAA 
CTAGCTGCTA AATACAGTTC TGGCATCAAC GGGACTCCAC CAGCAGACCC GCTTTCTCTC 
CCTAGCCCTA CAGCCACACT CCTGCAGGAG TGGAGCCAGA GGCCTGACTC AACCATTGGT 
GTTTTGGTTG CAAAGTTGCG AGAACTTGGC CGTCGAGACG CGGCAGATTT CCTGCTTAGA 
GCTGCACCAG TGTTTCGGGT GAACTTGGAG ATTTTGGGCA CCCCGTCTGA GCCCTACAGC 
CCCGTGTGTA ATGGAGGAGG CACATCCTAC AACTCCATCA GCTCCGTCAT ATCTCGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dapk1 ( XM_017356101.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017211590.1 Danio rerio death-associated protein kinase 1 (dapk1), transcript variant X2, mRNA.

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CloneID	ODa10713
Clone ID Related Accession (Same CDS sequence)	NM_001099990.2 , XM_017356101.1 , XM_017356100.2 , XM_017356100.1 , XM_017356101.2
Accession Version	XM_017356101.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4320bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	death-associated protein kinase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017356101.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGTGT TCAACCAGGA GAATGTGGAG GATTATTATG AGATTGGAGG AGAACTCGGG 
AGTGGTCAGT TTGCCGTGGT AAAGAAATGC CGTCATAAGA GCACTGGTGT GGAGTATGCA 
GCCAAATTTA TCAAGAAGAG GCGGAGCAAA TCAAGCCGAC GAGGGGTCTC AAAAGACGAC 
ATTGAGAGGG AGGTCAGCAT CCTGAAGGAA ATCCAGCACC CCAATGTCAT CACCCTGCAC 
GATGTGTTCG AAAACAAGCA TGAAGTCATC CTCATCCTAG AGCTTGTGGC TGGCGGAGAA 
CTCTTTGACT TTCTAGCAGA GAAAGAGTCA CTGAGTGAAG AGGAGGCCAC CGAGTTCCTC 
AAGCAGATCT TGGATGGAGT CAGTTACCTG CACTCGAAAC GGATTTCCCA CTTTGACCTG 
AAGCCGGAGA ACATCATGCT GTTAAACCGG AACGTGCCTC ACCCGCGCAT TAAGCTCATT 
GACTTTGGCC TGGCCCACAA GATCGATTTT GGCAACGATT TCAAAAACAT CTTTGGTACC 
CCGGAGTTTG TAGCTCCAGA AGTGGTGAAC TATGAGCCTC TTGGCCTGGA GGCAGACATG 
TGGAGCATTG GAGTAATCAC TTATATTCTG CTTAGCGGTG CTTCACCTTT CCTGGGTGAA 
AACAAGCAGG AGACGCTTGC CAATGTGTCA GCCGTGGACT ATGAGTTTGA TGAGGACTAT 
TTCAGCCACA CCAGCGCTTT GGCCAAAGAC TTCATTGCAC GGTTACTGAT CAAAGATCCA 
AAAAAAAGAA TGACAATCCT GGACAGTCTT CAACATCCCT GGATCAAGCC AAAGGATACA 
CAGCAGGCAC TGAGTCGCAA AGAATCAGCT GTCAATATGG AGAAATTCAA GAAGTTTGCT 
GCCCGAAGAA AATGGAAGCA ATCAGTGCGT CTCATCTCTC TGTGTAACCG GCTCTCCCGC 
TCCTTTCTGT CCAGGAGCAA CATGAGTGTG GCACGCAGTG ACGACACATT GGATGAAGAG 
GACTCGTTTG TCATGAAGGC TATCATCCAC GCAATAAACG ACGACAATGT TCCCGGTCTG 
AAACACCTAC TTGGATCTCT GACCAGCTAT GATATCAATC AACCTAACAA GCATGGAACT 
CCTCCGCTCC TGATCGCTGC AGGCTGTGGA AACGTCCAGA TCATTGATGT GCTCATGAAA 
AAAGGGGCTG AAATTCAAGC CTTTGATAAG ACTGGAGCCA GTGCTATCTA CTACGCCGCC 
CGACATGGTC ATGTGGGGAC CTTGAGATTC TTGCATGAAA AGAAGTGCCC TCTGGACATC 
CAAGATAAGT CCGGAGAAAC TGCTCTGCAT GTTGCGGCTC GCTATGGTAA TGTGGATGTG 
GTCCAATACC TTTGCAGCAT CCATGCCAAC CCTGACCTGT TGGACCGGGA GCAAGAGACG 
CCTCTCCATT GCGCGGCCTG GCACGGTTAT TCGGCAGTGG CCAGAGCCCT GTGTGAGGCT 
GGCTGTGATG TTAATGCCCG TAACCGTGAG GGTGAGAGTC CACTGCTAAC CGCTTCAGCC 
CGTGGCTTCA AGGACATTGT GGAGTGCTTG CTGGAGCATG GGGCTGACAT GGACTCTGCT 
GATAAGGATG GTCACATCGC TCTGCATCTA GCCGTGAGGA GGTGCCAGGT TGAGGTGGTC 
AAGTGTTTGC TCAGCCATCA CTGCTATGTG GACCACCAGG ATCGTCACGG CAACACACCC 
CTTCACATCG CCTGTAAAGA CGCCAACATG CCTGTGGTCA CGCAGCTCTG TGCTGCCAAA 
GCCAACCTGG ATATTCCCAA CAAATTTGGG AGAACACCTC TTCACCTGGC AGCGAGTAAC 
GGGGGTCTGG AGGTGGTGCG GCATCTCTGT CTTTCAGGAG CAAACACTGA GGCCATCACT 
AATGATGGAA AGACGGCGGA GGATCTGGCC ACTGCAGAGC AGCAGGAGCA TGTAGCAGTG 
CTGCTGGCCA AGCTGAAAAA GGACAACCAT AAGAGCATTT ACATCCAGCA GCTGCGGCCT 
ACACAGACCC TCCAACATCG CCTCAAACTC AAGCTGTTCG GCCATTCTGG AGCAGGGAAG 
AGCACTCTGG TGGAGTCCTT GAAGTGTGGC ATCTTGAGGA GCTTCTTCAG AAGGAGGAGG 
ACCAGGCTGA CCAACCCTGT CCGACACCCA GCCTCGCCGG TCACTTCCAA ACCTGCAGTG 
TCTGTGAGCA TCTCTAACCT GTATCCGGGG TGTGAGAATG TGAGTGTTCG GAGCCGCAGT 
ATGATGTTCG AGCCCAGTTT GACTAAAGGA GTGCTGGAGG TCTTCAGTCC TGTTCATAAT 
GCTCTCTCCA CGGCTGACGA CACTGCCACT AAAGCCATCG ACATCCAGAA CGCCAACGTC 
AATGGTGTTG GAGACTTCAG TATATGGGAG TTTTCTGGCA ATCCCGTCTA TTATTGCTCG 
TATGACTACT TCGCTGCCAA TGATGCCACA GCGATTCATC TGGTCTTATT CAGCCTGGAG 
GAGCCCTACG AAACTCAGCT CAACCAGGTC ACATTCTGGC TGAACTGTCT AAAGGCTCTC 
ACGCTGCCAG AGGACAACAT CGCCTTTGGA GGGAAGATCA GGAATCCTCT GCGTGTGGTT 
CTGGTGGCCA CACATGCTGA CATTGTGAAT CTGCCCCGAT CGTTTGGAGG AGAGTTCAGC 
TATGACAAGG AGAGATCTCT ACTGAAGGAA GTCAGAAACA GGTTTGGAAA TGATCTGCTG 
ATCACTGAGA AGCTCTTTGT CATGGATGCA GGAGCGTCCA ACTCTAAAGA CATAAAGCTT 
CTGAGAAACC TTCTGTTTGA GCTCCGCAGC ACCATCATTT CTACCTGCAG GCCGATGACT 
CAGCTGGCAG AGAAGCTGTT GTCCACTCTG CCTTCCTGGA GGAAACTGAA CGGGCCGAAC 
CAGCTCATAT CATGGCAACA GTTTGTTGTG GATGTGCAAG AACAAATCAA CCCTCTAGCC 
AGCGAAGAGG ATCTGCGGGG ACTGGCTTCA CAACTTCACA GCATGGGAGA GATTAACATC 
ATGCAGAGTG AGACAGTCCA AGATGTTGTC CTCTTAGATC CTCGCTGGCT CTGTAGTAAC 
GTACTTGGCA AGCTTCTCTC AATTGAAACC CCCAAAGCAA TCCACCATTA TCGCGGCCGA 
TACCGTGTAG AAGAGCTCCA ACACCTGGTC TCCGAAGGCG ACATCGATGA ACTTCTCCAA 
GTCCTCGACG CCATGGACGT CTGCGCCCGC GACCTGGCCA ACCCAGGAAT GGTGGACATC 
CCTGCCCTCA TTCGCACAGG TGGCCTGCAG CATACCTGGA CAGACGAGGA GGACGTGGAG 
GGAGAGGACG CTCTGCTTTA CGGTGGAGTC CGTATGGTTC CTGTGGAGCA CCTGGCACCC 
TTTCCTTGCG GACTTTTCCA TAAGTTACAG GTGAACTTGT GCAGGTGGAG CCGCCAGCAG 
AAGCCTGAGG TGGAGGCTGA TGATGTGAGG TTGTGGAGCG GTGGCGCTCG CATCGGACTT 
GGTGGTGTGG AGGCTCTGGT TCTGCTTGTG AACCACGGGC AAGGCATGGA GGTACAGTTA 
CGAGGACCTG ACACAGATCC GGGACGCTGC TATGCTTTGT TGGACACTCT GTGCGGCATG 
GCTGAGGGTC TGTTAGCTGC TACTTTGCCC GGTTTGCTTA CCGCCCGACA CTATCTCAGC 
CCTCAGCAAC TGCGTGAACA CCACGGCCCA GTCATGCTTT ACCAGCCGAG GGACTTTTTT 
AGAGCTCAAG CGCGAGGTGA GAACGCTCTG TCGAACACCA TGGGTGGCTA TCGCGAAAGC 
TTTGTCAGTA TCGTAGCATT AGGATGCATT CATGTCTACA AACAGGGCAA CCTCGGTGGC 
GAAGTGGCTG TAGCAGAGGT CAGTCTTTTG GCTCGACGTA AGCTCTGCCG CCTGCTAGAC 
CCGCCTGATG CCCTGGGCAA GGACTGGTGC CTGTTGGCCA TGAACTTGGG CCTTAGCGAA 
CTAGCTGCTA AATACAGTTC TGGCATCAAC GGGACTCCAC CAGCAGACCC GCTTTCTCTC 
CCTAGCCCTA CAGCCACACT CCTGCAGGAG TGGAGCCAGA GGCCTGACTC AACCATTGGT 
GTTTTGGTTG CAAAGTTGCG AGAACTTGGC CGTCGAGACG CGGCAGATTT CCTGCTTAGA 
GCTGCACCAG TGTTTCGGGT GAACTTGGAG ATTTTGGGCA CCCCGTCTGA GCCCTACAGC 
CCCGTGTGTA ATGGAGGAGG CACATCCTAC AACTCCATCA GCTCCGTCAT ATCTCGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017211590.1
CDS	251..4570
Translation	MTVFNQENVEDYYEIGGELGSGQFAVVKKCRHKSTGVEYAAKFIKKRRSKSSRRGVSKDDIEREVSILKEIQHPNVITLHDVFENKHEVILILELVAGGELFDFLAEKESLSEEEATEFLKQILDGVSYLHSKRISHFDLKPENIMLLNRNVPHPRIKLIDFGLAHKIDFGNDFKNIFGTPEFVAPEVVNYEPLGLEADMWSIGVITYILLSGASPFLGENKQETLANVSAVDYEFDEDYFSHTSALAKDFIARLLIKDPKKRMTILDSLQHPWIKPKDTQQALSRKESAVNMEKFKKFAARRKWKQSVRLISLCNRLSRSFLSRSNMSVARSDDTLDEEDSFVMKAIIHAINDDNVPGLKHLLGSLTSYDINQPNKHGTPPLLIAAGCGNVQIIDVLMKKGAEIQAFDKTGASAIYYAARHGHVGTLRFLHEKKCPLDIQDKSGETALHVAARYGNVDVVQYLCSIHANPDLLDREQETPLHCAAWHGYSAVARALCEAGCDVNARNREGESPLLTASARGFKDIVECLLEHGADMDSADKDGHIALHLAVRRCQVEVVKCLLSHHCYVDHQDRHGNTPLHIACKDANMPVVTQLCAAKANLDIPNKFGRTPLHLAASNGGLEVVRHLCLSGANTEAITNDGKTAEDLATAEQQEHVAVLLAKLKKDNHKSIYIQQLRPTQTLQHRLKLKLFGHSGAGKSTLVESLKCGILRSFFRRRRTRLTNPVRHPASPVTSKPAVSVSISNLYPGCENVSVRSRSMMFEPSLTKGVLEVFSPVHNALSTADDTATKAIDIQNANVNGVGDFSIWEFSGNPVYYCSYDYFAANDATAIHLVLFSLEEPYETQLNQVTFWLNCLKALTLPEDNIAFGGKIRNPLRVVLVATHADIVNLPRSFGGEFSYDKERSLLKEVRNRFGNDLLITEKLFVMDAGASNSKDIKLLRNLLFELRSTIISTCRPMTQLAEKLLSTLPSWRKLNGPNQLISWQQFVVDVQEQINPLASEEDLRGLASQLHSMGEINIMQSETVQDVVLLDPRWLCSNVLGKLLSIETPKAIHHYRGRYRVEELQHLVSEGDIDELLQVLDAMDVCARDLANPGMVDIPALIRTGGLQHTWTDEEDVEGEDALLYGGVRMVPVEHLAPFPCGLFHKLQVNLCRWSRQQKPEVEADDVRLWSGGARIGLGGVEALVLLVNHGQGMEVQLRGPDTDPGRCYALLDTLCGMAEGLLAATLPGLLTARHYLSPQQLREHHGPVMLYQPRDFFRAQARGENALSNTMGGYRESFVSIVALGCIHVYKQGNLGGEVAVAEVSLLARRKLCRLLDPPDALGKDWCLLAMNLGLSELAAKYSSGINGTPPADPLSLPSPTATLLQEWSQRPDSTIGVLVAKLRELGRRDAADFLLRAAPVFRVNLEILGTPSEPYSPVCNGGGTSYNSISSVISR

Target ORF information:

RefSeq Version	XM_017356101.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio death-associated protein kinase 1 (dapk1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356101.2

ORF Insert Sequence:

ATGACCGTGT TCAACCAGGA GAATGTGGAG GATTATTATG AGATTGGAGG AGAACTCGGG 
AGTGGTCAGT TTGCCGTGGT AAAGAAATGC CGTCATAAGA GCACTGGTGT GGAGTATGCA 
GCCAAATTTA TCAAGAAGAG GCGGAGCAAA TCAAGCCGAC GAGGGGTCTC AAAAGACGAC 
ATTGAGAGGG AGGTCAGCAT CCTGAAGGAA ATCCAGCACC CCAATGTCAT CACCCTGCAC 
GATGTGTTCG AAAACAAGCA TGAAGTCATC CTCATCCTAG AGCTTGTGGC TGGCGGAGAA 
CTCTTTGACT TTCTAGCAGA GAAAGAGTCA CTGAGTGAAG AGGAGGCCAC CGAGTTCCTC 
AAGCAGATCT TGGATGGAGT CAGTTACCTG CACTCGAAAC GGATTTCCCA CTTTGACCTG 
AAGCCGGAGA ACATCATGCT GTTAAACCGG AACGTGCCTC ACCCGCGCAT TAAGCTCATT 
GACTTTGGCC TGGCCCACAA GATCGATTTT GGCAACGATT TCAAAAACAT CTTTGGTACC 
CCGGAGTTTG TAGCTCCAGA AGTGGTGAAC TATGAGCCTC TTGGCCTGGA GGCAGACATG 
TGGAGCATTG GAGTAATCAC TTATATTCTG CTTAGCGGTG CTTCACCTTT CCTGGGTGAA 
AACAAGCAGG AGACGCTTGC CAATGTGTCA GCCGTGGACT ATGAGTTTGA TGAGGACTAT 
TTCAGCCACA CCAGCGCTTT GGCCAAAGAC TTCATTGCAC GGTTACTGAT CAAAGATCCA 
AAAAAAAGAA TGACAATCCT GGACAGTCTT CAACATCCCT GGATCAAGCC AAAGGATACA 
CAGCAGGCAC TGAGTCGCAA AGAATCAGCT GTCAATATGG AGAAATTCAA GAAGTTTGCT 
GCCCGAAGAA AATGGAAGCA ATCAGTGCGT CTCATCTCTC TGTGTAACCG GCTCTCCCGC 
TCCTTTCTGT CCAGGAGCAA CATGAGTGTG GCACGCAGTG ACGACACATT GGATGAAGAG 
GACTCGTTTG TCATGAAGGC TATCATCCAC GCAATAAACG ACGACAATGT TCCCGGTCTG 
AAACACCTAC TTGGATCTCT GACCAGCTAT GATATCAATC AACCTAACAA GCATGGAACT 
CCTCCGCTCC TGATCGCTGC AGGCTGTGGA AACGTCCAGA TCATTGATGT GCTCATGAAA 
AAAGGGGCTG AAATTCAAGC CTTTGATAAG ACTGGAGCCA GTGCTATCTA CTACGCCGCC 
CGACATGGTC ATGTGGGGAC CTTGAGATTC TTGCATGAAA AGAAGTGCCC TCTGGACATC 
CAAGATAAGT CCGGAGAAAC TGCTCTGCAT GTTGCGGCTC GCTATGGTAA TGTGGATGTG 
GTCCAATACC TTTGCAGCAT CCATGCCAAC CCTGACCTGT TGGACCGGGA GCAAGAGACG 
CCTCTCCATT GCGCGGCCTG GCACGGTTAT TCGGCAGTGG CCAGAGCCCT GTGTGAGGCT 
GGCTGTGATG TTAATGCCCG TAACCGTGAG GGTGAGAGTC CACTGCTAAC CGCTTCAGCC 
CGTGGCTTCA AGGACATTGT GGAGTGCTTG CTGGAGCATG GGGCTGACAT GGACTCTGCT 
GATAAGGATG GTCACATCGC TCTGCATCTA GCCGTGAGGA GGTGCCAGGT TGAGGTGGTC 
AAGTGTTTGC TCAGCCATCA CTGCTATGTG GACCACCAGG ATCGTCACGG CAACACACCC 
CTTCACATCG CCTGTAAAGA CGCCAACATG CCTGTGGTCA CGCAGCTCTG TGCTGCCAAA 
GCCAACCTGG ATATTCCCAA CAAATTTGGG AGAACACCTC TTCACCTGGC AGCGAGTAAC 
GGGGGTCTGG AGGTGGTGCG GCATCTCTGT CTTTCAGGAG CAAACACTGA GGCCATCACT 
AATGATGGAA AGACGGCGGA GGATCTGGCC ACTGCAGAGC AGCAGGAGCA TGTAGCAGTG 
CTGCTGGCCA AGCTGAAAAA GGACAACCAT AAGAGCATTT ACATCCAGCA GCTGCGGCCT 
ACACAGACCC TCCAACATCG CCTCAAACTC AAGCTGTTCG GCCATTCTGG AGCAGGGAAG 
AGCACTCTGG TGGAGTCCTT GAAGTGTGGC ATCTTGAGGA GCTTCTTCAG AAGGAGGAGG 
ACCAGGCTGA CCAACCCTGT CCGACACCCA GCCTCGCCGG TCACTTCCAA ACCTGCAGTG 
TCTGTGAGCA TCTCTAACCT GTATCCGGGG TGTGAGAATG TGAGTGTTCG GAGCCGCAGT 
ATGATGTTCG AGCCCAGTTT GACTAAAGGA GTGCTGGAGG TCTTCAGTCC TGTTCATAAT 
GCTCTCTCCA CGGCTGACGA CACTGCCACT AAAGCCATCG ACATCCAGAA CGCCAACGTC 
AATGGTGTTG GAGACTTCAG TATATGGGAG TTTTCTGGCA ATCCCGTCTA TTATTGCTCG 
TATGACTACT TCGCTGCCAA TGATGCCACA GCGATTCATC TGGTCTTATT CAGCCTGGAG 
GAGCCCTACG AAACTCAGCT CAACCAGGTC ACATTCTGGC TGAACTGTCT AAAGGCTCTC 
ACGCTGCCAG AGGACAACAT CGCCTTTGGA GGGAAGATCA GGAATCCTCT GCGTGTGGTT 
CTGGTGGCCA CACATGCTGA CATTGTGAAT CTGCCCCGAT CGTTTGGAGG AGAGTTCAGC 
TATGACAAGG AGAGATCTCT ACTGAAGGAA GTCAGAAACA GGTTTGGAAA TGATCTGCTG 
ATCACTGAGA AGCTCTTTGT CATGGATGCA GGAGCGTCCA ACTCTAAAGA CATAAAGCTT 
CTGAGAAACC TTCTGTTTGA GCTCCGCAGC ACCATCATTT CTACCTGCAG GCCGATGACT 
CAGCTGGCAG AGAAGCTGTT GTCCACTCTG CCTTCCTGGA GGAAACTGAA CGGGCCGAAC 
CAGCTCATAT CATGGCAACA GTTTGTTGTG GATGTGCAAG AACAAATCAA CCCTCTAGCC 
AGCGAAGAGG ATCTGCGGGG ACTGGCTTCA CAACTTCACA GCATGGGAGA GATTAACATC 
ATGCAGAGTG AGACAGTCCA AGATGTTGTC CTCTTAGATC CTCGCTGGCT CTGTAGTAAC 
GTACTTGGCA AGCTTCTCTC AATTGAAACC CCCAAAGCAA TCCACCATTA TCGCGGCCGA 
TACCGTGTAG AAGAGCTCCA ACACCTGGTC TCCGAAGGCG ACATCGATGA ACTTCTCCAA 
GTCCTCGACG CCATGGACGT CTGCGCCCGC GACCTGGCCA ACCCAGGAAT GGTGGACATC 
CCTGCCCTCA TTCGCACAGG TGGCCTGCAG CATACCTGGA CAGACGAGGA GGACGTGGAG 
GGAGAGGACG CTCTGCTTTA CGGTGGAGTC CGTATGGTTC CTGTGGAGCA CCTGGCACCC 
TTTCCTTGCG GACTTTTCCA TAAGTTACAG GTGAACTTGT GCAGGTGGAG CCGCCAGCAG 
AAGCCTGAGG TGGAGGCTGA TGATGTGAGG TTGTGGAGCG GTGGCGCTCG CATCGGACTT 
GGTGGTGTGG AGGCTCTGGT TCTGCTTGTG AACCACGGGC AAGGCATGGA GGTACAGTTA 
CGAGGACCTG ACACAGATCC GGGACGCTGC TATGCTTTGT TGGACACTCT GTGCGGCATG 
GCTGAGGGTC TGTTAGCTGC TACTTTGCCC GGTTTGCTTA CCGCCCGACA CTATCTCAGC 
CCTCAGCAAC TGCGTGAACA CCACGGCCCA GTCATGCTTT ACCAGCCGAG GGACTTTTTT 
AGAGCTCAAG CGCGAGGTGA GAACGCTCTG TCGAACACCA TGGGTGGCTA TCGCGAAAGC 
TTTGTCAGTA TCGTAGCATT AGGATGCATT CATGTCTACA AACAGGGCAA CCTCGGTGGC 
GAAGTGGCTG TAGCAGAGGT CAGTCTTTTG GCTCGACGTA AGCTCTGCCG CCTGCTAGAC 
CCGCCTGATG CCCTGGGCAA GGACTGGTGC CTGTTGGCCA TGAACTTGGG CCTTAGCGAA 
CTAGCTGCTA AATACAGTTC TGGCATCAAC GGGACTCCAC CAGCAGACCC GCTTTCTCTC 
CCTAGCCCTA CAGCCACACT CCTGCAGGAG TGGAGCCAGA GGCCTGACTC AACCATTGGT 
GTTTTGGTTG CAAAGTTGCG AGAACTTGGC CGTCGAGACG CGGCAGATTT CCTGCTTAGA 
GCTGCACCAG TGTTTCGGGT GAACTTGGAG ATTTTGGGCA CCCCGTCTGA GCCCTACAGC 
CCCGTGTGTA ATGGAGGAGG CACATCCTAC AACTCCATCA GCTCCGTCAT ATCTCGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A novel inducible mutagenesis screen enables to isolate and clone both embryonic and adult zebrafish mutants.
Scientific reports7(1)10381(2017 Sep)
Ma Z,Zhu P,Pang M,Guo L,Chang N,Zheng J,Zhu X,Gao C,Huang H,Cui Z,Xiong JW,Peng J,Chen J

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Evolutionary and developmental analysis reveals KANK genes were co-opted for vertebrate vascular development.
Scientific reports627816(2016 Jun)
Hensley MR,Cui Z,Chua RF,Simpson S,Shammas NL,Yang JY,Leung YF,Zhang G

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Interaction of infectious spleen and kidney necrosis virus ORF119L with PINCH leads to dominant-negative inhibition of integrin-linked kinase and cardiovascular defects in zebrafish.
Journal of virology89(1)763-75(2015 Jan)
Yuan JM,He BL,Yang LY,Guo CJ,Weng SP,Li SC,He JG

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dapk1 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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