Dapk1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dapk1
Entrez Gene ID	306722
Full Name	death associated protein kinase 1
General protein information	Preferred Names death associated protein kinase 1 Names death-associated protein kinase 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 17 Map Location: 17p14

mRNA and Protein(s)

mRNA	Protein	Name
XM_008771440.2	XP_008769662.1	death-associated protein kinase 1 isoform X1
XM_006253523.3	XP_006253585.1	death-associated protein kinase 1 isoform X2
NM_001107335.2	NP_001100805.2	death-associated protein kinase 1
XM_006253522.3	XP_006253584.1	death-associated protein kinase 1 isoform X1
XM_008771441.2	XP_008769663.1	death-associated protein kinase 1 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	DAPK1	NP_004929.2
Xenopus tropicalis (tropical clawed frog)	dapk1	NP_001116911.1
Rattus norvegicus (Norway rat)	Dapk1	NP_001100805.2
Danio rerio (zebrafish)	dapk1	NP_001093460.1
Pan troglodytes (chimpanzee)	DAPK1	XP_001140200.1
	DAPK1	XP_001084752.1
Bos taurus (cattle)	DAPK1	NP_001192967.1
Gallus gallus (chicken)	DAPK1	NP_001264960.1
Canis lupus familiaris (dog)	DAPK1	NP_001184086.1
Mus musculus (house mouse)	Dapk1	NP_598823.1
Caenorhabditis elegans (roundworm)	dapk-1	NP_490840.2

Gene Ontology
Bibliography

Related articles in PubMed

MicroRNA-141-3p targets DAPK1 and inhibits apoptosis in rat ovarian granulosa cells.
Li D, Xu D, Xu Y, Chen L, Li C, Dai X, Zhang L, Zheng L
Cell biochemistry and function35(4)197-201(2017 Jun)

Heterotrimeric GAIT complex drives transcript-selective translation inhibition in murine macrophages.
Arif A, Chatterjee P, Moodt RA, Fox PL
Molecular and cellular biology32(24)5046-55(2012 Dec)

PKD is a kinase of Vps34 that mediates ROS-induced autophagy downstream of DAPk.
Eisenberg-Lerner A, Kimchi A
Cell death and differentiation19(5)788-97(2012 May)

GTP binding to the ROC domain of DAP-kinase regulates its function through intramolecular signalling.
Carlessi R, Levin-Salomon V, Ciprut S, Bialik S, Berissi H, Albeck S, Peleg Y, Kimchi A
EMBO reports12(9)917-23(2011 Sep)

DAPK1 interaction with NMDA receptor NR2B subunits mediates brain damage in stroke.
Tu W, Xu X, Peng L, Zhong X, Zhang W, Soundarapandian MM, Balel C, Wang M, Jia N, Zhang W, Lew F, Chan SL, Chen Y, Lu Y
Cell140(2)222-34(2010 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

data indicate that miR-141-3p may inhibit ovarian granulosa cell apoptosis via targeting DAPK1 and is involved in the etiology of polycystic ovary syndrome.
Title: MicroRNA-141-3p targets DAPK1 and inhibits apoptosis in rat ovarian granulosa cells.

Results show that the serine/threonine kinase death-associated protein kinase (DAPK) is involved in the signal transduction of neogenin.
Title: Neogenin regulates neuronal survival through DAP kinase.

The following Dapk1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dapk1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa00071	NM_001107335.2 Latest version!	Rattus norvegicus death associated protein kinase 1 (Dapk1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
ORa00071	XM_006253523.3 Latest version!	Rattus norvegicus death associated protein kinase 1 (Dapk1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
ORa37632	XM_008771440.2 Latest version!	Rattus norvegicus death associated protein kinase 1 (Dapk1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
ORa37632	XM_008771441.2 Latest version!	Rattus norvegicus death associated protein kinase 1 (Dapk1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
ORa37632	XM_006253522.3 Latest version!	Rattus norvegicus death associated protein kinase 1 (Dapk1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dapk1 ( NM_001107335.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001100805.2 Rattus norvegicus death associated protein kinase 1 (Dapk1), mRNA.

Price & Availability↑

CloneID	ORa00071
Clone ID Related Accession (Same CDS sequence)	XM_006253523.3 , NM_001107335.2
Accession Version	NM_001107335.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4293bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-27
Organism	Rattus norvegicus(Norway rat)
Product	death-associated protein kinase 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07026911.1, AABR07026910.1 and AABR07026909.1. On Oct 29, 2008 this sequence version replaced NM_001107335.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACTGTGT TCAGGCAGGA AAACGTGGAC GACTACTACG ACACCGGCGA GGAACTGGGC 
AGCGGACAGT TCGCCGTTGT GAAGAAATGT CGTGAGAAAA GTACCGGTCT TCAGTATGCG 
GCCAAGTTCA TCAAGAAAAG GAGGACCAAG TCCAGCCGGC GGGGCGTGAG CCGAGAGGAC 
ATCGAGCGGG AGGTCAGCAT CCTGAAGGAG ATCCGGCACC CAAACGTCAT CACCCTGCAT 
GAGGTCTACG AGAACAAGAC AGACGTCATT CTGATCCTGG AGCTTGTTGC AGGAGGCGAG 
CTGTTTGACT TCCTGGCTGA GAAGGAATCT CTCACTGAAG AAGAAGCAAC CGAGTTCCTC 
AAGCAGATCC TCAGCGGCGT TTACTACCTG CACTCGCTTC AAATCGCCCA CTTTGACCTG 
AAGCCGGAAA ACATAATGCT TCTGGATAGA AATGTGCCCA AACCTCGGAT CAAGATCATC 
GACTTCGGCC TGGCCCATAA AATTGACTTT GGAAATGAAT TCAAAAACAT ATTTGGGACA 
CCAGAGTTTG TGGCTCCGGA GATCGTCAAC TATGAGCCCC TTGGTCTTGA GGCAGACATG 
TGGAGCATTG GGGTAATAAC CTATATCCTC CTAAGCGGGG CCTCCCCTTT TCTTGGAGAC 
ACCAAGCAAG AAACATTAGC GAATGTATCT GCTGTCAACT ATGACTTTGA GGAGGAATTC 
TTCCGCAACA CCAGTACCCT TGCCAAAGAT TTCATCAGAA GGCTGCTGGT CAAGGATCCA 
AAGAAGAGGA TGACAATCCA GGACAGTTTG CAGCACCCCT GGATCAAGCC TAAAGACACC 
CAACAAGCAC TTAGTCGAAA AGCCTCAGCA GTAAACATGG AGAAATTCAA GAAGTTTGCA 
GCTCGGAAAA AATGGAAACA ATCTGTTCGC TTGATATCAC TGTGCCAAAG ATTATCCAGG 
TCATTTTTGT CCAGAAGTAA CATGAGCGTT GCCAGGAGCG ATGATACTCT GGATGAGGAA 
GACTCCTTTG TGATGAAAGC CATCATTCAT GCCATCAATG ATGACAACGT CCCCGGCCTG 
CAGCACCTCC TGGGCTCATT GTCCAGCTAT GATGTCAACC AGCCCAACAA GCACGGGACA 
CCTCCATTAC TGATTGCTGC AGGCTGTGGG AATATTCAGA TGTTACAGTT ACTCATAAAA 
CGGGGCTCAA GGATCGACGT CCAGGATAAG GGAGGATCCA ATGCTATCTA CTGGGCCTCT 
CGGCATGGCC ATGTGGATAC TTTGAAGTTT CTCAATGAGA ACAAATGCCC TTTGGATGTT 
AAAGACAAGT CTGGAGAGAC AGCTCTTCAC GTGGCAGCCC GCTATGGCCA TGCAGATGTG 
GTTCAACTCT TGTGCAGTTT TGGCTCTAAT CCTGATTTCC AGGACAAGGA AGAGGAAACC 
CCCCTGCACT GTGCTGCCTG GCACGGCTAT TACTCCGTGG CTAGAGCTCT TTGTGAAGTT 
GGCTGTAACG TGAATATCAA GAACCGGGAG GGAGAGACCC CACTGCTGAC AGCTTCTGCC 
AGGGGCTACC ATGACATTGT GGAGTGTCTG GCTGAACATG GAGCTGACCT GAATGCTTCT 
GACAAGGATG GGCACATCGC CCTTCATCTT GCTGTGAGGC GCTGTCAGAT GGAGGTCATC 
AAGACCCTCC TTGGCCATGG GTGCCTTGTG GATTTCCAGG ATCGGCATGG CAACACCCCC 
CTGCATGTGG CCTGCAAAGA TGGGAGCGCG CCCATCGTGG TGGCCCTCTG TGAAGCCAGC 
TGCAACCTGG ACATCTCAAA CAAGTATGGT CGGACTCCTC TCCACCTTGC AGCCAACAAC 
GGGATCCTAG ATGTGGTCCG CTACCTCTGC CTGATGGGCG CCAACGTGGA GGCTCTGACC 
TTGGATGGCA AGACGGCCGA GGACCTCGCC AAGGCAGAGC AGCACGAGCA CGTGGCAGGG 
CTCCTGGCAA GACTGCGGAA GGACACACAC CGAGGGCTCT TCATCCAGCA GCTCCGACCC 
ACGCAGAATC TCCAGCCCAG AATCAAGCTC AAACTGTTTG GCCACTCGGG ATCGGGGAAA 
TCCACACTGG TGGAATCTCT CAAGTGTGGG CTGTTAAGGA GTTTCTTCAG AAGGCGCCGG 
CCCAGACTGT CCTCTACCAA CTCCACCCGC TTCCCACCGT CGCCCCTGGC TACTAAGCCA 
ACAGTCTCAG TGAGCATTAA CAACCTGTAC CCCGGCTGTG AGAATGTGAG CGTGAGGAGC 
CGAAGCATGA TGTTTGAGCC AGGCCTCACC AAAGGGATGC TGGAAGTGTT CGTGGCTCCG 
TCTCACCACC TACACTGCTC GACCGATGAC CAGTCCACCA AAGCCATCGA CATCCAGAAT 
GCTTACTTGA ACGGAGTTGG TGATTTCAGC GTATGGGAGT TCTCTGGAAA CCCTGTGTAC 
TTCTGTTGCT ATGACTACTT CGCCGCCAAC GATCCCACAT CCATCCACAT CATCGTTTTC 
AGTCTCGAAG AACCCTATGA GATCCAGCTG AACCAAGTGA TTTTCTGGCT TAGTTTCTTG 
AAGTCTCTGG TCTCAGTTGA AGAACCCATA GCATTTGGAG GCAAGCTGAA GAACCCTCTC 
CGAGTTGTCC TGGTGGCCAC ACATGCTGAC ATCATGAACA TCCCTCGGCC TGCTGGGGGC 
GAGTTTGGAT ATGATAAAGA TACATCCTTG CTGAAAGAGA TCAGGAACAG GTTTGGGAAT 
GACCTTCATG TCTCAAATAA GCTGTTTGTG CTGGATGCAG GAGCATCTGG GTCTAAGGAC 
ATCAAGGTTC TCCGGAATCA CCTGCAAGAA ATACGGAGTC AGATTGTCTC GGGGTGCCCT 
CCCATGACTC ATCTGTGTGA GAAGATCATC TCCACTCTGC CCTCCTGGCG GAAGCTCAAC 
GGGCCAAACC AGCTGATGTC GCTCCAGCAG TTTGTGTATG ACGTGCAGGA CCAGCTGAAC 
CCCCTGGCCT CCGAGGATGA CCTCAGGCGC ATTGCACAGC AGCTGCACAG CACGGGAGAG 
ATCAACATCA TGCAGAGTGA GACAGTGCAG GATGTGTTGC TGCTGGATCC TCGATGGCTC 
TGCACCAACG TCCTGGGGAA GCTGCTGTCT GTGGAGACTC CCCGAGCCCT GCACCATTAT 
CGGGGCCGCT ACACCATGGA AGATATCCAG CGTCTGGTCC CCGACAGTGA CGTGGAGGAG 
CTGCTGCAGA TCCTGGATGC CATGGATATC TGCGCCCGAG ACCTGAGTAG CGGGACTATG 
GTGGATATCC CTGCTCTAAT CAAAACAGAC AGCCTGCAGC GCTCCTGGGC CGATGAGGAA 
GACGAGGTGA TGGTGTACGG AGGTGTGCGC ATCGTCCCTG TAGAGCACCT CACTCCCTTC 
CCCTGTGGCA TCTTTCACAA AGTTCAGGTC AACCTGTGCC GGTGGATCCA CCAGCAGAGC 
ACTGAGGGGG ACGCGGACAT CCGTCTGTGG GTGAGCGGCT GCAGGATCGC CAACCGTGGG 
GCTGAGTTGC TGGTGCTGCT GGTCAATCAC GGTCAGGGCA TTGAGGTACA GGTGCGTGGA 
CTGGAGACCG AGAAGATTAA GTGCTGCCTG CTGCTGGACT CGGTGTGCAG CACCATCGAG 
ACTGTCATGG CCACCACCTT GCCAGGGCTG TTGACGGTGA AGCACTACCT GAGCCCCCAG 
CAGCTAAGGG AGCATCACGA ACCAGTCATG GTCTACCAGC CCCGGGACTT CTTCCGTGCG 
CAGACCCTGA AAGAGAGCTC CCTCACCAAC ACCATGGGAG GGTACAAGGA GAGCTTCAGC 
AGCATCACGT GCTTTGGGTG TCACGATGTC TACTCACAGG CCAGTCTTGG CATGGACATC 
CATGCATCAG ACCTGAGTCT CCTGACCCGG AGGAAACTGA GTCGTCTTCT TGACCCACCG 
GACCCCATGG GGAAGGACTG GTGCCTTCTG GCCATGAACT TGGGCCTCCC GGACATGGTG 
GCCAAACACA ACGTCAATAC CAGGGCTTCT AGGGATTTCC TCCCCAGCCC AGTGCATGCC 
TTGCTACAGG AATGGACCTC CTACCCTGAG AGCACGGTGG GCATCCTTAT ATCCAAACTT 
CGGGAGCTGG GGCGCCGGGA TGCTGCGGAC TTTTTACTGA AGGCCTCCTC TGTGTTCAAG 
ATCAACCTTG ACGGCAATGG CCAGGAGGCC TATGCCTCAA GCTGTAACAG TGGCACATCC 
TACAATTCCA TAAGCTCAGT GGTGTCCCGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001100805.2
CDS	275..4567
Translation	MTVFRQENVDDYYDTGEELGSGQFAVVKKCREKSTGLQYAAKFIKKRRTKSSRRGVSREDIEREVSILKEIRHPNVITLHEVYENKTDVILILELVAGGELFDFLAEKESLTEEEATEFLKQILSGVYYLHSLQIAHFDLKPENIMLLDRNVPKPRIKIIDFGLAHKIDFGNEFKNIFGTPEFVAPEIVNYEPLGLEADMWSIGVITYILLSGASPFLGDTKQETLANVSAVNYDFEEEFFRNTSTLAKDFIRRLLVKDPKKRMTIQDSLQHPWIKPKDTQQALSRKASAVNMEKFKKFAARKKWKQSVRLISLCQRLSRSFLSRSNMSVARSDDTLDEEDSFVMKAIIHAINDDNVPGLQHLLGSLSSYDVNQPNKHGTPPLLIAAGCGNIQMLQLLIKRGSRIDVQDKGGSNAIYWASRHGHVDTLKFLNENKCPLDVKDKSGETALHVAARYGHADVVQLLCSFGSNPDFQDKEEETPLHCAAWHGYYSVARALCEVGCNVNIKNREGETPLLTASARGYHDIVECLAEHGADLNASDKDGHIALHLAVRRCQMEVIKTLLGHGCLVDFQDRHGNTPLHVACKDGSAPIVVALCEASCNLDISNKYGRTPLHLAANNGILDVVRYLCLMGANVEALTLDGKTAEDLAKAEQHEHVAGLLARLRKDTHRGLFIQQLRPTQNLQPRIKLKLFGHSGSGKSTLVESLKCGLLRSFFRRRRPRLSSTNSTRFPPSPLATKPTVSVSINNLYPGCENVSVRSRSMMFEPGLTKGMLEVFVAPSHHLHCSTDDQSTKAIDIQNAYLNGVGDFSVWEFSGNPVYFCCYDYFAANDPTSIHIIVFSLEEPYEIQLNQVIFWLSFLKSLVSVEEPIAFGGKLKNPLRVVLVATHADIMNIPRPAGGEFGYDKDTSLLKEIRNRFGNDLHVSNKLFVLDAGASGSKDIKVLRNHLQEIRSQIVSGCPPMTHLCEKIISTLPSWRKLNGPNQLMSLQQFVYDVQDQLNPLASEDDLRRIAQQLHSTGEINIMQSETVQDVLLLDPRWLCTNVLGKLLSVETPRALHHYRGRYTMEDIQRLVPDSDVEELLQILDAMDICARDLSSGTMVDIPALIKTDSLQRSWADEEDEVMVYGGVRIVPVEHLTPFPCGIFHKVQVNLCRWIHQQSTEGDADIRLWVSGCRIANRGAELLVLLVNHGQGIEVQVRGLETEKIKCCLLLDSVCSTIETVMATTLPGLLTVKHYLSPQQLREHHEPVMVYQPRDFFRAQTLKESSLTNTMGGYKESFSSITCFGCHDVYSQASLGMDIHASDLSLLTRRKLSRLLDPPDPMGKDWCLLAMNLGLPDMVAKHNVNTRASRDFLPSPVHALLQEWTSYPESTVGILISKLRELGRRDAADFLLKASSVFKINLDGNGQEAYASSCNSGTSYNSISSVVSR

Target ORF information:

RefSeq Version	NM_001107335.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus death associated protein kinase 1 (Dapk1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001107335.2

ORF Insert Sequence:

ATGACTGTGT TCAGGCAGGA AAACGTGGAC GACTACTACG ACACCGGCGA GGAACTGGGC 
AGCGGACAGT TCGCCGTTGT GAAGAAATGT CGTGAGAAAA GTACCGGTCT TCAGTATGCG 
GCCAAGTTCA TCAAGAAAAG GAGGACCAAG TCCAGCCGGC GGGGCGTGAG CCGAGAGGAC 
ATCGAGCGGG AGGTCAGCAT CCTGAAGGAG ATCCGGCACC CAAACGTCAT CACCCTGCAT 
GAGGTCTACG AGAACAAGAC AGACGTCATT CTGATCCTGG AGCTTGTTGC AGGAGGCGAG 
CTGTTTGACT TCCTGGCTGA GAAGGAATCT CTCACTGAAG AAGAAGCAAC CGAGTTCCTC 
AAGCAGATCC TCAGCGGCGT TTACTACCTG CACTCGCTTC AAATCGCCCA CTTTGACCTG 
AAGCCGGAAA ACATAATGCT TCTGGATAGA AATGTGCCCA AACCTCGGAT CAAGATCATC 
GACTTCGGCC TGGCCCATAA AATTGACTTT GGAAATGAAT TCAAAAACAT ATTTGGGACA 
CCAGAGTTTG TGGCTCCGGA GATCGTCAAC TATGAGCCCC TTGGTCTTGA GGCAGACATG 
TGGAGCATTG GGGTAATAAC CTATATCCTC CTAAGCGGGG CCTCCCCTTT TCTTGGAGAC 
ACCAAGCAAG AAACATTAGC GAATGTATCT GCTGTCAACT ATGACTTTGA GGAGGAATTC 
TTCCGCAACA CCAGTACCCT TGCCAAAGAT TTCATCAGAA GGCTGCTGGT CAAGGATCCA 
AAGAAGAGGA TGACAATCCA GGACAGTTTG CAGCACCCCT GGATCAAGCC TAAAGACACC 
CAACAAGCAC TTAGTCGAAA AGCCTCAGCA GTAAACATGG AGAAATTCAA GAAGTTTGCA 
GCTCGGAAAA AATGGAAACA ATCTGTTCGC TTGATATCAC TGTGCCAAAG ATTATCCAGG 
TCATTTTTGT CCAGAAGTAA CATGAGCGTT GCCAGGAGCG ATGATACTCT GGATGAGGAA 
GACTCCTTTG TGATGAAAGC CATCATTCAT GCCATCAATG ATGACAACGT CCCCGGCCTG 
CAGCACCTCC TGGGCTCATT GTCCAGCTAT GATGTCAACC AGCCCAACAA GCACGGGACA 
CCTCCATTAC TGATTGCTGC AGGCTGTGGG AATATTCAGA TGTTACAGTT ACTCATAAAA 
CGGGGCTCAA GGATCGACGT CCAGGATAAG GGAGGATCCA ATGCTATCTA CTGGGCCTCT 
CGGCATGGCC ATGTGGATAC TTTGAAGTTT CTCAATGAGA ACAAATGCCC TTTGGATGTT 
AAAGACAAGT CTGGAGAGAC AGCTCTTCAC GTGGCAGCCC GCTATGGCCA TGCAGATGTG 
GTTCAACTCT TGTGCAGTTT TGGCTCTAAT CCTGATTTCC AGGACAAGGA AGAGGAAACC 
CCCCTGCACT GTGCTGCCTG GCACGGCTAT TACTCCGTGG CTAGAGCTCT TTGTGAAGTT 
GGCTGTAACG TGAATATCAA GAACCGGGAG GGAGAGACCC CACTGCTGAC AGCTTCTGCC 
AGGGGCTACC ATGACATTGT GGAGTGTCTG GCTGAACATG GAGCTGACCT GAATGCTTCT 
GACAAGGATG GGCACATCGC CCTTCATCTT GCTGTGAGGC GCTGTCAGAT GGAGGTCATC 
AAGACCCTCC TTGGCCATGG GTGCCTTGTG GATTTCCAGG ATCGGCATGG CAACACCCCC 
CTGCATGTGG CCTGCAAAGA TGGGAGCGCG CCCATCGTGG TGGCCCTCTG TGAAGCCAGC 
TGCAACCTGG ACATCTCAAA CAAGTATGGT CGGACTCCTC TCCACCTTGC AGCCAACAAC 
GGGATCCTAG ATGTGGTCCG CTACCTCTGC CTGATGGGCG CCAACGTGGA GGCTCTGACC 
TTGGATGGCA AGACGGCCGA GGACCTCGCC AAGGCAGAGC AGCACGAGCA CGTGGCAGGG 
CTCCTGGCAA GACTGCGGAA GGACACACAC CGAGGGCTCT TCATCCAGCA GCTCCGACCC 
ACGCAGAATC TCCAGCCCAG AATCAAGCTC AAACTGTTTG GCCACTCGGG ATCGGGGAAA 
TCCACACTGG TGGAATCTCT CAAGTGTGGG CTGTTAAGGA GTTTCTTCAG AAGGCGCCGG 
CCCAGACTGT CCTCTACCAA CTCCACCCGC TTCCCACCGT CGCCCCTGGC TACTAAGCCA 
ACAGTCTCAG TGAGCATTAA CAACCTGTAC CCCGGCTGTG AGAATGTGAG CGTGAGGAGC 
CGAAGCATGA TGTTTGAGCC AGGCCTCACC AAAGGGATGC TGGAAGTGTT CGTGGCTCCG 
TCTCACCACC TACACTGCTC GACCGATGAC CAGTCCACCA AAGCCATCGA CATCCAGAAT 
GCTTACTTGA ACGGAGTTGG TGATTTCAGC GTATGGGAGT TCTCTGGAAA CCCTGTGTAC 
TTCTGTTGCT ATGACTACTT CGCCGCCAAC GATCCCACAT CCATCCACAT CATCGTTTTC 
AGTCTCGAAG AACCCTATGA GATCCAGCTG AACCAAGTGA TTTTCTGGCT TAGTTTCTTG 
AAGTCTCTGG TCTCAGTTGA AGAACCCATA GCATTTGGAG GCAAGCTGAA GAACCCTCTC 
CGAGTTGTCC TGGTGGCCAC ACATGCTGAC ATCATGAACA TCCCTCGGCC TGCTGGGGGC 
GAGTTTGGAT ATGATAAAGA TACATCCTTG CTGAAAGAGA TCAGGAACAG GTTTGGGAAT 
GACCTTCATG TCTCAAATAA GCTGTTTGTG CTGGATGCAG GAGCATCTGG GTCTAAGGAC 
ATCAAGGTTC TCCGGAATCA CCTGCAAGAA ATACGGAGTC AGATTGTCTC GGGGTGCCCT 
CCCATGACTC ATCTGTGTGA GAAGATCATC TCCACTCTGC CCTCCTGGCG GAAGCTCAAC 
GGGCCAAACC AGCTGATGTC GCTCCAGCAG TTTGTGTATG ACGTGCAGGA CCAGCTGAAC 
CCCCTGGCCT CCGAGGATGA CCTCAGGCGC ATTGCACAGC AGCTGCACAG CACGGGAGAG 
ATCAACATCA TGCAGAGTGA GACAGTGCAG GATGTGTTGC TGCTGGATCC TCGATGGCTC 
TGCACCAACG TCCTGGGGAA GCTGCTGTCT GTGGAGACTC CCCGAGCCCT GCACCATTAT 
CGGGGCCGCT ACACCATGGA AGATATCCAG CGTCTGGTCC CCGACAGTGA CGTGGAGGAG 
CTGCTGCAGA TCCTGGATGC CATGGATATC TGCGCCCGAG ACCTGAGTAG CGGGACTATG 
GTGGATATCC CTGCTCTAAT CAAAACAGAC AGCCTGCAGC GCTCCTGGGC CGATGAGGAA 
GACGAGGTGA TGGTGTACGG AGGTGTGCGC ATCGTCCCTG TAGAGCACCT CACTCCCTTC 
CCCTGTGGCA TCTTTCACAA AGTTCAGGTC AACCTGTGCC GGTGGATCCA CCAGCAGAGC 
ACTGAGGGGG ACGCGGACAT CCGTCTGTGG GTGAGCGGCT GCAGGATCGC CAACCGTGGG 
GCTGAGTTGC TGGTGCTGCT GGTCAATCAC GGTCAGGGCA TTGAGGTACA GGTGCGTGGA 
CTGGAGACCG AGAAGATTAA GTGCTGCCTG CTGCTGGACT CGGTGTGCAG CACCATCGAG 
ACTGTCATGG CCACCACCTT GCCAGGGCTG TTGACGGTGA AGCACTACCT GAGCCCCCAG 
CAGCTAAGGG AGCATCACGA ACCAGTCATG GTCTACCAGC CCCGGGACTT CTTCCGTGCG 
CAGACCCTGA AAGAGAGCTC CCTCACCAAC ACCATGGGAG GGTACAAGGA GAGCTTCAGC 
AGCATCACGT GCTTTGGGTG TCACGATGTC TACTCACAGG CCAGTCTTGG CATGGACATC 
CATGCATCAG ACCTGAGTCT CCTGACCCGG AGGAAACTGA GTCGTCTTCT TGACCCACCG 
GACCCCATGG GGAAGGACTG GTGCCTTCTG GCCATGAACT TGGGCCTCCC GGACATGGTG 
GCCAAACACA ACGTCAATAC CAGGGCTTCT AGGGATTTCC TCCCCAGCCC AGTGCATGCC 
TTGCTACAGG AATGGACCTC CTACCCTGAG AGCACGGTGG GCATCCTTAT ATCCAAACTT 
CGGGAGCTGG GGCGCCGGGA TGCTGCGGAC TTTTTACTGA AGGCCTCCTC TGTGTTCAAG 
ATCAACCTTG ACGGCAATGG CCAGGAGGCC TATGCCTCAA GCTGTAACAG TGGCACATCC 
TACAATTCCA TAAGCTCAGT GGTGTCCCGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dapk1 ( XM_006253523.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006253585.1 Rattus norvegicus death associated protein kinase 1 (Dapk1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa00071
Clone ID Related Accession (Same CDS sequence)	XM_006253523.3 , NM_001107335.2
Accession Version	XM_006253523.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4293bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	death-associated protein kinase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005116.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006253523.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGTGT TCAGGCAGGA AAACGTGGAC GACTACTACG ACACCGGCGA GGAACTGGGC 
AGCGGACAGT TCGCCGTTGT GAAGAAATGT CGTGAGAAAA GTACCGGTCT TCAGTATGCG 
GCCAAGTTCA TCAAGAAAAG GAGGACCAAG TCCAGCCGGC GGGGCGTGAG CCGAGAGGAC 
ATCGAGCGGG AGGTCAGCAT CCTGAAGGAG ATCCGGCACC CAAACGTCAT CACCCTGCAT 
GAGGTCTACG AGAACAAGAC AGACGTCATT CTGATCCTGG AGCTTGTTGC AGGAGGCGAG 
CTGTTTGACT TCCTGGCTGA GAAGGAATCT CTCACTGAAG AAGAAGCAAC CGAGTTCCTC 
AAGCAGATCC TCAGCGGCGT TTACTACCTG CACTCGCTTC AAATCGCCCA CTTTGACCTG 
AAGCCGGAAA ACATAATGCT TCTGGATAGA AATGTGCCCA AACCTCGGAT CAAGATCATC 
GACTTCGGCC TGGCCCATAA AATTGACTTT GGAAATGAAT TCAAAAACAT ATTTGGGACA 
CCAGAGTTTG TGGCTCCGGA GATCGTCAAC TATGAGCCCC TTGGTCTTGA GGCAGACATG 
TGGAGCATTG GGGTAATAAC CTATATCCTC CTAAGCGGGG CCTCCCCTTT TCTTGGAGAC 
ACCAAGCAAG AAACATTAGC GAATGTATCT GCTGTCAACT ATGACTTTGA GGAGGAATTC 
TTCCGCAACA CCAGTACCCT TGCCAAAGAT TTCATCAGAA GGCTGCTGGT CAAGGATCCA 
AAGAAGAGGA TGACAATCCA GGACAGTTTG CAGCACCCCT GGATCAAGCC TAAAGACACC 
CAACAAGCAC TTAGTCGAAA AGCCTCAGCA GTAAACATGG AGAAATTCAA GAAGTTTGCA 
GCTCGGAAAA AATGGAAACA ATCTGTTCGC TTGATATCAC TGTGCCAAAG ATTATCCAGG 
TCATTTTTGT CCAGAAGTAA CATGAGCGTT GCCAGGAGCG ATGATACTCT GGATGAGGAA 
GACTCCTTTG TGATGAAAGC CATCATTCAT GCCATCAATG ATGACAACGT CCCCGGCCTG 
CAGCACCTCC TGGGCTCATT GTCCAGCTAT GATGTCAACC AGCCCAACAA GCACGGGACA 
CCTCCATTAC TGATTGCTGC AGGCTGTGGG AATATTCAGA TGTTACAGTT ACTCATAAAA 
CGGGGCTCAA GGATCGACGT CCAGGATAAG GGAGGATCCA ATGCTATCTA CTGGGCCTCT 
CGGCATGGCC ATGTGGATAC TTTGAAGTTT CTCAATGAGA ACAAATGCCC TTTGGATGTT 
AAAGACAAGT CTGGAGAGAC AGCTCTTCAC GTGGCAGCCC GCTATGGCCA TGCAGATGTG 
GTTCAACTCT TGTGCAGTTT TGGCTCTAAT CCTGATTTCC AGGACAAGGA AGAGGAAACC 
CCCCTGCACT GTGCTGCCTG GCACGGCTAT TACTCCGTGG CTAGAGCTCT TTGTGAAGTT 
GGCTGTAACG TGAATATCAA GAACCGGGAG GGAGAGACCC CACTGCTGAC AGCTTCTGCC 
AGGGGCTACC ATGACATTGT GGAGTGTCTG GCTGAACATG GAGCTGACCT GAATGCTTCT 
GACAAGGATG GGCACATCGC CCTTCATCTT GCTGTGAGGC GCTGTCAGAT GGAGGTCATC 
AAGACCCTCC TTGGCCATGG GTGCCTTGTG GATTTCCAGG ATCGGCATGG CAACACCCCC 
CTGCATGTGG CCTGCAAAGA TGGGAGCGCG CCCATCGTGG TGGCCCTCTG TGAAGCCAGC 
TGCAACCTGG ACATCTCAAA CAAGTATGGT CGGACTCCTC TCCACCTTGC AGCCAACAAC 
GGGATCCTAG ATGTGGTCCG CTACCTCTGC CTGATGGGCG CCAACGTGGA GGCTCTGACC 
TTGGATGGCA AGACGGCCGA GGACCTCGCC AAGGCAGAGC AGCACGAGCA CGTGGCAGGG 
CTCCTGGCAA GACTGCGGAA GGACACACAC CGAGGGCTCT TCATCCAGCA GCTCCGACCC 
ACGCAGAATC TCCAGCCCAG AATCAAGCTC AAACTGTTTG GCCACTCGGG ATCGGGGAAA 
TCCACACTGG TGGAATCTCT CAAGTGTGGG CTGTTAAGGA GTTTCTTCAG AAGGCGCCGG 
CCCAGACTGT CCTCTACCAA CTCCACCCGC TTCCCACCGT CGCCCCTGGC TACTAAGCCA 
ACAGTCTCAG TGAGCATTAA CAACCTGTAC CCCGGCTGTG AGAATGTGAG CGTGAGGAGC 
CGAAGCATGA TGTTTGAGCC AGGCCTCACC AAAGGGATGC TGGAAGTGTT CGTGGCTCCG 
TCTCACCACC TACACTGCTC GACCGATGAC CAGTCCACCA AAGCCATCGA CATCCAGAAT 
GCTTACTTGA ACGGAGTTGG TGATTTCAGC GTATGGGAGT TCTCTGGAAA CCCTGTGTAC 
TTCTGTTGCT ATGACTACTT CGCCGCCAAC GATCCCACAT CCATCCACAT CATCGTTTTC 
AGTCTCGAAG AACCCTATGA GATCCAGCTG AACCAAGTGA TTTTCTGGCT TAGTTTCTTG 
AAGTCTCTGG TCTCAGTTGA AGAACCCATA GCATTTGGAG GCAAGCTGAA GAACCCTCTC 
CGAGTTGTCC TGGTGGCCAC ACATGCTGAC ATCATGAACA TCCCTCGGCC TGCTGGGGGC 
GAGTTTGGAT ATGATAAAGA TACATCCTTG CTGAAAGAGA TCAGGAACAG GTTTGGGAAT 
GACCTTCATG TCTCAAATAA GCTGTTTGTG CTGGATGCAG GAGCATCTGG GTCTAAGGAC 
ATCAAGGTTC TCCGGAATCA CCTGCAAGAA ATACGGAGTC AGATTGTCTC GGGGTGCCCT 
CCCATGACTC ATCTGTGTGA GAAGATCATC TCCACTCTGC CCTCCTGGCG GAAGCTCAAC 
GGGCCAAACC AGCTGATGTC GCTCCAGCAG TTTGTGTATG ACGTGCAGGA CCAGCTGAAC 
CCCCTGGCCT CCGAGGATGA CCTCAGGCGC ATTGCACAGC AGCTGCACAG CACGGGAGAG 
ATCAACATCA TGCAGAGTGA GACAGTGCAG GATGTGTTGC TGCTGGATCC TCGATGGCTC 
TGCACCAACG TCCTGGGGAA GCTGCTGTCT GTGGAGACTC CCCGAGCCCT GCACCATTAT 
CGGGGCCGCT ACACCATGGA AGATATCCAG CGTCTGGTCC CCGACAGTGA CGTGGAGGAG 
CTGCTGCAGA TCCTGGATGC CATGGATATC TGCGCCCGAG ACCTGAGTAG CGGGACTATG 
GTGGATATCC CTGCTCTAAT CAAAACAGAC AGCCTGCAGC GCTCCTGGGC CGATGAGGAA 
GACGAGGTGA TGGTGTACGG AGGTGTGCGC ATCGTCCCTG TAGAGCACCT CACTCCCTTC 
CCCTGTGGCA TCTTTCACAA AGTTCAGGTC AACCTGTGCC GGTGGATCCA CCAGCAGAGC 
ACTGAGGGGG ACGCGGACAT CCGTCTGTGG GTGAGCGGCT GCAGGATCGC CAACCGTGGG 
GCTGAGTTGC TGGTGCTGCT GGTCAATCAC GGTCAGGGCA TTGAGGTACA GGTGCGTGGA 
CTGGAGACCG AGAAGATTAA GTGCTGCCTG CTGCTGGACT CGGTGTGCAG CACCATCGAG 
ACTGTCATGG CCACCACCTT GCCAGGGCTG TTGACGGTGA AGCACTACCT GAGCCCCCAG 
CAGCTAAGGG AGCATCACGA ACCAGTCATG GTCTACCAGC CCCGGGACTT CTTCCGTGCG 
CAGACCCTGA AAGAGAGCTC CCTCACCAAC ACCATGGGAG GGTACAAGGA GAGCTTCAGC 
AGCATCACGT GCTTTGGGTG TCACGATGTC TACTCACAGG CCAGTCTTGG CATGGACATC 
CATGCATCAG ACCTGAGTCT CCTGACCCGG AGGAAACTGA GTCGTCTTCT TGACCCACCG 
GACCCCATGG GGAAGGACTG GTGCCTTCTG GCCATGAACT TGGGCCTCCC GGACATGGTG 
GCCAAACACA ACGTCAATAC CAGGGCTTCT AGGGATTTCC TCCCCAGCCC AGTGCATGCC 
TTGCTACAGG AATGGACCTC CTACCCTGAG AGCACGGTGG GCATCCTTAT ATCCAAACTT 
CGGGAGCTGG GGCGCCGGGA TGCTGCGGAC TTTTTACTGA AGGCCTCCTC TGTGTTCAAG 
ATCAACCTTG ACGGCAATGG CCAGGAGGCC TATGCCTCAA GCTGTAACAG TGGCACATCC 
TACAATTCCA TAAGCTCAGT GGTGTCCCGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006253585.1
CDS	204..4496
Translation	MTVFRQENVDDYYDTGEELGSGQFAVVKKCREKSTGLQYAAKFIKKRRTKSSRRGVSREDIEREVSILKEIRHPNVITLHEVYENKTDVILILELVAGGELFDFLAEKESLTEEEATEFLKQILSGVYYLHSLQIAHFDLKPENIMLLDRNVPKPRIKIIDFGLAHKIDFGNEFKNIFGTPEFVAPEIVNYEPLGLEADMWSIGVITYILLSGASPFLGDTKQETLANVSAVNYDFEEEFFRNTSTLAKDFIRRLLVKDPKKRMTIQDSLQHPWIKPKDTQQALSRKASAVNMEKFKKFAARKKWKQSVRLISLCQRLSRSFLSRSNMSVARSDDTLDEEDSFVMKAIIHAINDDNVPGLQHLLGSLSSYDVNQPNKHGTPPLLIAAGCGNIQMLQLLIKRGSRIDVQDKGGSNAIYWASRHGHVDTLKFLNENKCPLDVKDKSGETALHVAARYGHADVVQLLCSFGSNPDFQDKEEETPLHCAAWHGYYSVARALCEVGCNVNIKNREGETPLLTASARGYHDIVECLAEHGADLNASDKDGHIALHLAVRRCQMEVIKTLLGHGCLVDFQDRHGNTPLHVACKDGSAPIVVALCEASCNLDISNKYGRTPLHLAANNGILDVVRYLCLMGANVEALTLDGKTAEDLAKAEQHEHVAGLLARLRKDTHRGLFIQQLRPTQNLQPRIKLKLFGHSGSGKSTLVESLKCGLLRSFFRRRRPRLSSTNSTRFPPSPLATKPTVSVSINNLYPGCENVSVRSRSMMFEPGLTKGMLEVFVAPSHHLHCSTDDQSTKAIDIQNAYLNGVGDFSVWEFSGNPVYFCCYDYFAANDPTSIHIIVFSLEEPYEIQLNQVIFWLSFLKSLVSVEEPIAFGGKLKNPLRVVLVATHADIMNIPRPAGGEFGYDKDTSLLKEIRNRFGNDLHVSNKLFVLDAGASGSKDIKVLRNHLQEIRSQIVSGCPPMTHLCEKIISTLPSWRKLNGPNQLMSLQQFVYDVQDQLNPLASEDDLRRIAQQLHSTGEINIMQSETVQDVLLLDPRWLCTNVLGKLLSVETPRALHHYRGRYTMEDIQRLVPDSDVEELLQILDAMDICARDLSSGTMVDIPALIKTDSLQRSWADEEDEVMVYGGVRIVPVEHLTPFPCGIFHKVQVNLCRWIHQQSTEGDADIRLWVSGCRIANRGAELLVLLVNHGQGIEVQVRGLETEKIKCCLLLDSVCSTIETVMATTLPGLLTVKHYLSPQQLREHHEPVMVYQPRDFFRAQTLKESSLTNTMGGYKESFSSITCFGCHDVYSQASLGMDIHASDLSLLTRRKLSRLLDPPDPMGKDWCLLAMNLGLPDMVAKHNVNTRASRDFLPSPVHALLQEWTSYPESTVGILISKLRELGRRDAADFLLKASSVFKINLDGNGQEAYASSCNSGTSYNSISSVVSR

Target ORF information:

RefSeq Version	XM_006253523.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus death associated protein kinase 1 (Dapk1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006253523.3

ORF Insert Sequence:

ATGACTGTGT TCAGGCAGGA AAACGTGGAC GACTACTACG ACACCGGCGA GGAACTGGGC 
AGCGGACAGT TCGCCGTTGT GAAGAAATGT CGTGAGAAAA GTACCGGTCT TCAGTATGCG 
GCCAAGTTCA TCAAGAAAAG GAGGACCAAG TCCAGCCGGC GGGGCGTGAG CCGAGAGGAC 
ATCGAGCGGG AGGTCAGCAT CCTGAAGGAG ATCCGGCACC CAAACGTCAT CACCCTGCAT 
GAGGTCTACG AGAACAAGAC AGACGTCATT CTGATCCTGG AGCTTGTTGC AGGAGGCGAG 
CTGTTTGACT TCCTGGCTGA GAAGGAATCT CTCACTGAAG AAGAAGCAAC CGAGTTCCTC 
AAGCAGATCC TCAGCGGCGT TTACTACCTG CACTCGCTTC AAATCGCCCA CTTTGACCTG 
AAGCCGGAAA ACATAATGCT TCTGGATAGA AATGTGCCCA AACCTCGGAT CAAGATCATC 
GACTTCGGCC TGGCCCATAA AATTGACTTT GGAAATGAAT TCAAAAACAT ATTTGGGACA 
CCAGAGTTTG TGGCTCCGGA GATCGTCAAC TATGAGCCCC TTGGTCTTGA GGCAGACATG 
TGGAGCATTG GGGTAATAAC CTATATCCTC CTAAGCGGGG CCTCCCCTTT TCTTGGAGAC 
ACCAAGCAAG AAACATTAGC GAATGTATCT GCTGTCAACT ATGACTTTGA GGAGGAATTC 
TTCCGCAACA CCAGTACCCT TGCCAAAGAT TTCATCAGAA GGCTGCTGGT CAAGGATCCA 
AAGAAGAGGA TGACAATCCA GGACAGTTTG CAGCACCCCT GGATCAAGCC TAAAGACACC 
CAACAAGCAC TTAGTCGAAA AGCCTCAGCA GTAAACATGG AGAAATTCAA GAAGTTTGCA 
GCTCGGAAAA AATGGAAACA ATCTGTTCGC TTGATATCAC TGTGCCAAAG ATTATCCAGG 
TCATTTTTGT CCAGAAGTAA CATGAGCGTT GCCAGGAGCG ATGATACTCT GGATGAGGAA 
GACTCCTTTG TGATGAAAGC CATCATTCAT GCCATCAATG ATGACAACGT CCCCGGCCTG 
CAGCACCTCC TGGGCTCATT GTCCAGCTAT GATGTCAACC AGCCCAACAA GCACGGGACA 
CCTCCATTAC TGATTGCTGC AGGCTGTGGG AATATTCAGA TGTTACAGTT ACTCATAAAA 
CGGGGCTCAA GGATCGACGT CCAGGATAAG GGAGGATCCA ATGCTATCTA CTGGGCCTCT 
CGGCATGGCC ATGTGGATAC TTTGAAGTTT CTCAATGAGA ACAAATGCCC TTTGGATGTT 
AAAGACAAGT CTGGAGAGAC AGCTCTTCAC GTGGCAGCCC GCTATGGCCA TGCAGATGTG 
GTTCAACTCT TGTGCAGTTT TGGCTCTAAT CCTGATTTCC AGGACAAGGA AGAGGAAACC 
CCCCTGCACT GTGCTGCCTG GCACGGCTAT TACTCCGTGG CTAGAGCTCT TTGTGAAGTT 
GGCTGTAACG TGAATATCAA GAACCGGGAG GGAGAGACCC CACTGCTGAC AGCTTCTGCC 
AGGGGCTACC ATGACATTGT GGAGTGTCTG GCTGAACATG GAGCTGACCT GAATGCTTCT 
GACAAGGATG GGCACATCGC CCTTCATCTT GCTGTGAGGC GCTGTCAGAT GGAGGTCATC 
AAGACCCTCC TTGGCCATGG GTGCCTTGTG GATTTCCAGG ATCGGCATGG CAACACCCCC 
CTGCATGTGG CCTGCAAAGA TGGGAGCGCG CCCATCGTGG TGGCCCTCTG TGAAGCCAGC 
TGCAACCTGG ACATCTCAAA CAAGTATGGT CGGACTCCTC TCCACCTTGC AGCCAACAAC 
GGGATCCTAG ATGTGGTCCG CTACCTCTGC CTGATGGGCG CCAACGTGGA GGCTCTGACC 
TTGGATGGCA AGACGGCCGA GGACCTCGCC AAGGCAGAGC AGCACGAGCA CGTGGCAGGG 
CTCCTGGCAA GACTGCGGAA GGACACACAC CGAGGGCTCT TCATCCAGCA GCTCCGACCC 
ACGCAGAATC TCCAGCCCAG AATCAAGCTC AAACTGTTTG GCCACTCGGG ATCGGGGAAA 
TCCACACTGG TGGAATCTCT CAAGTGTGGG CTGTTAAGGA GTTTCTTCAG AAGGCGCCGG 
CCCAGACTGT CCTCTACCAA CTCCACCCGC TTCCCACCGT CGCCCCTGGC TACTAAGCCA 
ACAGTCTCAG TGAGCATTAA CAACCTGTAC CCCGGCTGTG AGAATGTGAG CGTGAGGAGC 
CGAAGCATGA TGTTTGAGCC AGGCCTCACC AAAGGGATGC TGGAAGTGTT CGTGGCTCCG 
TCTCACCACC TACACTGCTC GACCGATGAC CAGTCCACCA AAGCCATCGA CATCCAGAAT 
GCTTACTTGA ACGGAGTTGG TGATTTCAGC GTATGGGAGT TCTCTGGAAA CCCTGTGTAC 
TTCTGTTGCT ATGACTACTT CGCCGCCAAC GATCCCACAT CCATCCACAT CATCGTTTTC 
AGTCTCGAAG AACCCTATGA GATCCAGCTG AACCAAGTGA TTTTCTGGCT TAGTTTCTTG 
AAGTCTCTGG TCTCAGTTGA AGAACCCATA GCATTTGGAG GCAAGCTGAA GAACCCTCTC 
CGAGTTGTCC TGGTGGCCAC ACATGCTGAC ATCATGAACA TCCCTCGGCC TGCTGGGGGC 
GAGTTTGGAT ATGATAAAGA TACATCCTTG CTGAAAGAGA TCAGGAACAG GTTTGGGAAT 
GACCTTCATG TCTCAAATAA GCTGTTTGTG CTGGATGCAG GAGCATCTGG GTCTAAGGAC 
ATCAAGGTTC TCCGGAATCA CCTGCAAGAA ATACGGAGTC AGATTGTCTC GGGGTGCCCT 
CCCATGACTC ATCTGTGTGA GAAGATCATC TCCACTCTGC CCTCCTGGCG GAAGCTCAAC 
GGGCCAAACC AGCTGATGTC GCTCCAGCAG TTTGTGTATG ACGTGCAGGA CCAGCTGAAC 
CCCCTGGCCT CCGAGGATGA CCTCAGGCGC ATTGCACAGC AGCTGCACAG CACGGGAGAG 
ATCAACATCA TGCAGAGTGA GACAGTGCAG GATGTGTTGC TGCTGGATCC TCGATGGCTC 
TGCACCAACG TCCTGGGGAA GCTGCTGTCT GTGGAGACTC CCCGAGCCCT GCACCATTAT 
CGGGGCCGCT ACACCATGGA AGATATCCAG CGTCTGGTCC CCGACAGTGA CGTGGAGGAG 
CTGCTGCAGA TCCTGGATGC CATGGATATC TGCGCCCGAG ACCTGAGTAG CGGGACTATG 
GTGGATATCC CTGCTCTAAT CAAAACAGAC AGCCTGCAGC GCTCCTGGGC CGATGAGGAA 
GACGAGGTGA TGGTGTACGG AGGTGTGCGC ATCGTCCCTG TAGAGCACCT CACTCCCTTC 
CCCTGTGGCA TCTTTCACAA AGTTCAGGTC AACCTGTGCC GGTGGATCCA CCAGCAGAGC 
ACTGAGGGGG ACGCGGACAT CCGTCTGTGG GTGAGCGGCT GCAGGATCGC CAACCGTGGG 
GCTGAGTTGC TGGTGCTGCT GGTCAATCAC GGTCAGGGCA TTGAGGTACA GGTGCGTGGA 
CTGGAGACCG AGAAGATTAA GTGCTGCCTG CTGCTGGACT CGGTGTGCAG CACCATCGAG 
ACTGTCATGG CCACCACCTT GCCAGGGCTG TTGACGGTGA AGCACTACCT GAGCCCCCAG 
CAGCTAAGGG AGCATCACGA ACCAGTCATG GTCTACCAGC CCCGGGACTT CTTCCGTGCG 
CAGACCCTGA AAGAGAGCTC CCTCACCAAC ACCATGGGAG GGTACAAGGA GAGCTTCAGC 
AGCATCACGT GCTTTGGGTG TCACGATGTC TACTCACAGG CCAGTCTTGG CATGGACATC 
CATGCATCAG ACCTGAGTCT CCTGACCCGG AGGAAACTGA GTCGTCTTCT TGACCCACCG 
GACCCCATGG GGAAGGACTG GTGCCTTCTG GCCATGAACT TGGGCCTCCC GGACATGGTG 
GCCAAACACA ACGTCAATAC CAGGGCTTCT AGGGATTTCC TCCCCAGCCC AGTGCATGCC 
TTGCTACAGG AATGGACCTC CTACCCTGAG AGCACGGTGG GCATCCTTAT ATCCAAACTT 
CGGGAGCTGG GGCGCCGGGA TGCTGCGGAC TTTTTACTGA AGGCCTCCTC TGTGTTCAAG 
ATCAACCTTG ACGGCAATGG CCAGGAGGCC TATGCCTCAA GCTGTAACAG TGGCACATCC 
TACAATTCCA TAAGCTCAGT GGTGTCCCGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dapk1 ( XM_008771440.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008769662.1 Rattus norvegicus death associated protein kinase 1 (Dapk1), transcript variant X2, mRNA.

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CloneID	ORa37632
Clone ID Related Accession (Same CDS sequence)	XM_008771440.2 , XM_006253522.3 , XM_008771441.2
Accession Version	XM_008771440.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4329bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	death-associated protein kinase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005116.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008771440.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGTGT TCAGGCAGGA AAACGTGGAC GACTACTACG ACACCGGCGA GGAACTGGGC 
AGCGGACAGT TCGCCGTTGT GAAGAAATGT CGTGAGAAAA GTACCGGTCT TCAGTATGCG 
GCCAAGTTCA TCAAGAAAAG GAGGACCAAG TCCAGCCGGC GGGGCGTGAG CCGAGAGGAC 
ATCGAGCGGG AGGTCAGCAT CCTGAAGGAG ATCCGGCACC CAAACGTCAT CACCCTGCAT 
GAGGTCTACG AGAACAAGAC AGACGTCATT CTGATCCTGG AGCTTGTTGC AGGAGGCGAG 
CTGTTTGACT TCCTGGCTGA GAAGGAATCT CTCACTGAAG AAGAAGCAAC CGAGTTCCTC 
AAGCAGATCC TCAGCGGCGT TTACTACCTG CACTCGCTTC AAATCGCCCA CTTTGACCTG 
AAGCCGGAAA ACATAATGCT TCTGGATAGA AATGTGCCCA AACCTCGGAT CAAGATCATC 
GACTTCGGCC TGGCCCATAA AATTGACTTT GGAAATGAAT TCAAAAACAT ATTTGGGACA 
CCAGAGTTTG TGGCTCCGGA GATCGTCAAC TATGAGCCCC TTGGTCTTGA GGCAGACATG 
TGGAGCATTG GGGTAATAAC CTATATCCTC CTAAGCGGGG CCTCCCCTTT TCTTGGAGAC 
ACCAAGCAAG AAACATTAGC GAATGTATCT GCTGTCAACT ATGACTTTGA GGAGGAATTC 
TTCCGCAACA CCAGTACCCT TGCCAAAGAT TTCATCAGAA GGCTGCTGGT CAAGGATCCA 
AAGAAGAGGA TGACAATCCA GGACAGTTTG CAGCACCCCT GGATCAAGCC TAAAGACACC 
CAACAAGCAC TTAGTCGAAA AGCCTCAGCA GTAAACATGG AGAAATTCAA GAAGTTTGCA 
GCTCGGAAAA AATGGAAACA ATCTGTTCGC TTGATATCAC TGTGCCAAAG ATTATCCAGG 
TCATTTTTGT CCAGAAGTAA CATGAGCGTT GCCAGGAGCG ATGATACTCT GGATGAGGAA 
GACTCCTTTG TGATGAAAGC CATCATTCAT GCCATCAATG ATGACAACGT CCCCGGCCTG 
CAGCACCTCC TGGGCTCATT GTCCAGCTAT GATGTCAACC AGCCCAACAA GCACGGGACA 
CCTCCATTAC TGATTGCTGC AGGCTGTGGG AATATTCAGA TGTTACAGTT ACTCATAAAA 
CGGGGCTCAA GGATCGACGT CCAGGATAAG GGAGGATCCA ATGCTATCTA CTGGGCCTCT 
CGGCATGGCC ATGTGGATAC TTTGAAGTTT CTCAATGAGA ACAAATGCCC TTTGGATGTT 
AAAGACAAGT CTGGAGAGAC AGCTCTTCAC GTGGCAGCCC GCTATGGCCA TGCAGATGTG 
GTTCAACTCT TGTGCAGTTT TGGCTCTAAT CCTGATTTCC AGGACAAGGA AGAGGAAACC 
CCCCTGCACT GTGCTGCCTG GCACGGCTAT TACTCCGTGG CTAGAGCTCT TTGTGAAGTT 
GGCTGTAACG TGAATATCAA GAACCGGGAG GGAGAGACCC CACTGCTGAC AGCTTCTGCC 
AGGGGCTACC ATGACATTGT GGAGTGTCTG GCTGAACATG GAGCTGACCT GAATGCTTCT 
GACAAGGATG GGCACATCGC CCTTCATCTT GCTGTGAGGC GCTGTCAGAT GGAGGTCATC 
AAGACCCTCC TTGGCCATGG GTGCCTTGTG GATTTCCAGG ATCGGCATGG CAACACCCCC 
CTGCATGTGG CCTGCAAAGA TGGGAGCGCG CCCATCGTGG TGGCCCTCTG TGAAGCCAGC 
TGCAACCTGG ACATCTCAAA CAAGTATGGT CGGACTCCTC TCCACCTTGC AGCCAACAAC 
GGGATCCTAG ATGTGGTCCG CTACCTCTGC CTGATGGGCG CCAACGTGGA GGCTCTGACC 
TTGGATGGCA AGACGGCCGA GGACCTCGCC AAGGCAGAGC AGCACGAGCA CGTGGCAGGG 
CTCCTGGCAA GACTGCGGAA GGACACACAC CGAGGGCTCT TCATCCAGCA GCTCCGACCC 
ACGCAGAATC TCCAGCCCAG AATCAAGCTC AAACTGTTTG GCCACTCGGG ATCGGGGAAA 
TCCACACTGG TGGAATCTCT CAAGTGTGGG CTGTTAAGGA GTTTCTTCAG AAGGCGCCGG 
CCCAGACTGT CCTCTACCAA CTCCACCCGC TTCCCACCGT CGCCCCTGGC TACTAAGCCA 
ACAGTCTCAG TGAGCATTAA CAACCTGTAC CCCGGCTGTG AGAATGTGAG CGTGAGGAGC 
CGAAGCATGA TGTTTGAGCC AGGCCTCACC AAAGGGATGC TGGAAGTGTT CGTGGCTCCG 
TCTCACCACC TACACTGCTC GACCGATGAC CAGTCCACCA AAGCCATCGA CATCCAGAAT 
GCTTACTTGA ACGGAGTTGG TGATTTCAGC GTATGGGAGT TCTCTGGAAA CCCTGTGTAC 
TTCTGTTGCT ATGACTACTT CGCCGCCAAC GATCCCACAT CCATCCACAT CATCGTTTTC 
AGTCTCGAAG AACCCTATGA GATCCAGCTG AACCAAGTGA TTTTCTGGCT TAGTTTCTTG 
AAGTCTCTGG TCTCAGTTGA AGAACCCATA GCATTTGGAG GCAAGCTGAA GAACCCTCTC 
CGAGTTGTCC TGGTGGCCAC ACATGCTGAC ATCATGAACA TCCCTCGGCC TGCTGGGGGC 
GAGTTTGGAT ATGATAAAGA TACATCCTTG CTGAAAGAGA TCAGGAACAG GTTTGGGAAT 
GACCTTCATG TCTCAAATAA GCTGTTTGTG CTGGATGCAG GAGCATCTGG GTCTAAGGAC 
ATCAAGGTTC TCCGGAATCA CCTGCAAGAA ATACGGAGTC AGATTGTCTC GGGGTGCCCT 
CCCATGACTC ATCTGTGTGA GAAGATCATC TCCACTCTGC CCTCCTGGCG GAAGCTCAAC 
GGGCCAAACC AGCTGATGTC GCTCCAGCAG TTTGTGTATG ACGTGCAGGA CCAGCTGAAC 
CCCCTGGCCT CCGAGGATGA CCTCAGGCGC ATTGCACAGC AGCTGCACAG CACGGGAGAG 
ATCAACATCA TGCAGAGTGA GACAGTGCAG GATGTGTTGC TGCTGGATCC TCGATGGCTC 
TGCACCAACG TCCTGGGGAA GCTGCTGTCT GTGGAGACTC CCCGAGCCCT GCACCATTAT 
CGGGGCCGCT ACACCATGGA AGATATCCAG CGTCTGGTCC CCGACAGTGA CGTGGAGGAG 
CTGCTGCAGA TCCTGGATGC CATGGATATC TGCGCCCGAG ACCTGAGTAG CGGGACTATG 
GTGGATATCC CTGCTCTAAT CAAAACAGAC AGCCTGCAGC GCTCCTGGGC CGATGAGGAA 
GACGAGGTGA TGGTGTACGG AGGTGTGCGC ATCGTCCCTG TAGAGCACCT CACTCCCTTC 
CCCTGTGGCA TCTTTCACAA AGTTCAGGTC AACCTGTGCC GGTGGATCCA CCAGCAGAGC 
ACTGAGGGGG ACGCGGACAT CCGTCTGTGG GTGAGCGGCT GCAGGATCGC CAACCGTGGG 
GCTGAGTTGC TGGTGCTGCT GGTCAATCAC GGTCAGGGCA TTGAGGTACA GGTGCGTGGA 
CTGGAGACCG AGAAGATTAA GTGCTGCCTG CTGCTGGACT CGGTGTGCAG CACCATCGAG 
ACTGTCATGG CCACCACCTT GCCAGGGCTG TTGACGGTGA AGCACTACCT GAGCCCCCAG 
CAGCTAAGGG AGCATCACGA ACCAGTCATG GTCTACCAGC CCCGGGACTT CTTCCGTGCG 
CAGACCCTGA AAGAGAGCTC CCTCACCAAC ACCATGGGAG GGTACAAGGA GAGCTTCAGC 
AGCATCACGT GCTTTGGGTG TCACGATGTC TACTCACAGG CCAGTCTTGG CATGGACATC 
CATGCATCAG ACCTGAGTCT CCTGACCCGG AGGAAACTGA GTCGTCTTCT TGACCCACCG 
GACCCCATGG GGAAGGACTG GTGCCTTCTG GCCATGAACT TGGGCCTCCC GGACATGGTG 
GCCAAACACA ACGTCAATAC CAGGGCTTCT AGGGATTTCC TCCCCAGCCC AGTGCATGCC 
TTGCTACAGG AATGGACCTC CTACCCTGAG AGCACGGTGG GCATCCTTAT ATCCAAACTT 
CGGGAGCTGG GGCGCCGGGA TGCTGCGGAC TTTTTACTGA AGGCCTCCTC TGTGTTCAAG 
ATCAACCTTG ACGGCAATGG CCAGGAGGCC TATGCCTCAA GCTGTAACAG TGGCACATCC 
TACAATTCCA TAAGCTCAGT GGTGTCCCGG AGAGACTCCC ATGCTTGGAC CCCACTGTAT 
GATTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008769662.1
CDS	203..4531
Translation	MTVFRQENVDDYYDTGEELGSGQFAVVKKCREKSTGLQYAAKFIKKRRTKSSRRGVSREDIEREVSILKEIRHPNVITLHEVYENKTDVILILELVAGGELFDFLAEKESLTEEEATEFLKQILSGVYYLHSLQIAHFDLKPENIMLLDRNVPKPRIKIIDFGLAHKIDFGNEFKNIFGTPEFVAPEIVNYEPLGLEADMWSIGVITYILLSGASPFLGDTKQETLANVSAVNYDFEEEFFRNTSTLAKDFIRRLLVKDPKKRMTIQDSLQHPWIKPKDTQQALSRKASAVNMEKFKKFAARKKWKQSVRLISLCQRLSRSFLSRSNMSVARSDDTLDEEDSFVMKAIIHAINDDNVPGLQHLLGSLSSYDVNQPNKHGTPPLLIAAGCGNIQMLQLLIKRGSRIDVQDKGGSNAIYWASRHGHVDTLKFLNENKCPLDVKDKSGETALHVAARYGHADVVQLLCSFGSNPDFQDKEEETPLHCAAWHGYYSVARALCEVGCNVNIKNREGETPLLTASARGYHDIVECLAEHGADLNASDKDGHIALHLAVRRCQMEVIKTLLGHGCLVDFQDRHGNTPLHVACKDGSAPIVVALCEASCNLDISNKYGRTPLHLAANNGILDVVRYLCLMGANVEALTLDGKTAEDLAKAEQHEHVAGLLARLRKDTHRGLFIQQLRPTQNLQPRIKLKLFGHSGSGKSTLVESLKCGLLRSFFRRRRPRLSSTNSTRFPPSPLATKPTVSVSINNLYPGCENVSVRSRSMMFEPGLTKGMLEVFVAPSHHLHCSTDDQSTKAIDIQNAYLNGVGDFSVWEFSGNPVYFCCYDYFAANDPTSIHIIVFSLEEPYEIQLNQVIFWLSFLKSLVSVEEPIAFGGKLKNPLRVVLVATHADIMNIPRPAGGEFGYDKDTSLLKEIRNRFGNDLHVSNKLFVLDAGASGSKDIKVLRNHLQEIRSQIVSGCPPMTHLCEKIISTLPSWRKLNGPNQLMSLQQFVYDVQDQLNPLASEDDLRRIAQQLHSTGEINIMQSETVQDVLLLDPRWLCTNVLGKLLSVETPRALHHYRGRYTMEDIQRLVPDSDVEELLQILDAMDICARDLSSGTMVDIPALIKTDSLQRSWADEEDEVMVYGGVRIVPVEHLTPFPCGIFHKVQVNLCRWIHQQSTEGDADIRLWVSGCRIANRGAELLVLLVNHGQGIEVQVRGLETEKIKCCLLLDSVCSTIETVMATTLPGLLTVKHYLSPQQLREHHEPVMVYQPRDFFRAQTLKESSLTNTMGGYKESFSSITCFGCHDVYSQASLGMDIHASDLSLLTRRKLSRLLDPPDPMGKDWCLLAMNLGLPDMVAKHNVNTRASRDFLPSPVHALLQEWTSYPESTVGILISKLRELGRRDAADFLLKASSVFKINLDGNGQEAYASSCNSGTSYNSISSVVSRRDSHAWTPLYDL

Target ORF information:

RefSeq Version	XM_008771440.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus death associated protein kinase 1 (Dapk1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008771440.2

ORF Insert Sequence:

ATGACTGTGT TCAGGCAGGA AAACGTGGAC GACTACTACG ACACCGGCGA GGAACTGGGC 
AGCGGACAGT TCGCCGTTGT GAAGAAATGT CGTGAGAAAA GTACCGGTCT TCAGTATGCG 
GCCAAGTTCA TCAAGAAAAG GAGGACCAAG TCCAGCCGGC GGGGCGTGAG CCGAGAGGAC 
ATCGAGCGGG AGGTCAGCAT CCTGAAGGAG ATCCGGCACC CAAACGTCAT CACCCTGCAT 
GAGGTCTACG AGAACAAGAC AGACGTCATT CTGATCCTGG AGCTTGTTGC AGGAGGCGAG 
CTGTTTGACT TCCTGGCTGA GAAGGAATCT CTCACTGAAG AAGAAGCAAC CGAGTTCCTC 
AAGCAGATCC TCAGCGGCGT TTACTACCTG CACTCGCTTC AAATCGCCCA CTTTGACCTG 
AAGCCGGAAA ACATAATGCT TCTGGATAGA AATGTGCCCA AACCTCGGAT CAAGATCATC 
GACTTCGGCC TGGCCCATAA AATTGACTTT GGAAATGAAT TCAAAAACAT ATTTGGGACA 
CCAGAGTTTG TGGCTCCGGA GATCGTCAAC TATGAGCCCC TTGGTCTTGA GGCAGACATG 
TGGAGCATTG GGGTAATAAC CTATATCCTC CTAAGCGGGG CCTCCCCTTT TCTTGGAGAC 
ACCAAGCAAG AAACATTAGC GAATGTATCT GCTGTCAACT ATGACTTTGA GGAGGAATTC 
TTCCGCAACA CCAGTACCCT TGCCAAAGAT TTCATCAGAA GGCTGCTGGT CAAGGATCCA 
AAGAAGAGGA TGACAATCCA GGACAGTTTG CAGCACCCCT GGATCAAGCC TAAAGACACC 
CAACAAGCAC TTAGTCGAAA AGCCTCAGCA GTAAACATGG AGAAATTCAA GAAGTTTGCA 
GCTCGGAAAA AATGGAAACA ATCTGTTCGC TTGATATCAC TGTGCCAAAG ATTATCCAGG 
TCATTTTTGT CCAGAAGTAA CATGAGCGTT GCCAGGAGCG ATGATACTCT GGATGAGGAA 
GACTCCTTTG TGATGAAAGC CATCATTCAT GCCATCAATG ATGACAACGT CCCCGGCCTG 
CAGCACCTCC TGGGCTCATT GTCCAGCTAT GATGTCAACC AGCCCAACAA GCACGGGACA 
CCTCCATTAC TGATTGCTGC AGGCTGTGGG AATATTCAGA TGTTACAGTT ACTCATAAAA 
CGGGGCTCAA GGATCGACGT CCAGGATAAG GGAGGATCCA ATGCTATCTA CTGGGCCTCT 
CGGCATGGCC ATGTGGATAC TTTGAAGTTT CTCAATGAGA ACAAATGCCC TTTGGATGTT 
AAAGACAAGT CTGGAGAGAC AGCTCTTCAC GTGGCAGCCC GCTATGGCCA TGCAGATGTG 
GTTCAACTCT TGTGCAGTTT TGGCTCTAAT CCTGATTTCC AGGACAAGGA AGAGGAAACC 
CCCCTGCACT GTGCTGCCTG GCACGGCTAT TACTCCGTGG CTAGAGCTCT TTGTGAAGTT 
GGCTGTAACG TGAATATCAA GAACCGGGAG GGAGAGACCC CACTGCTGAC AGCTTCTGCC 
AGGGGCTACC ATGACATTGT GGAGTGTCTG GCTGAACATG GAGCTGACCT GAATGCTTCT 
GACAAGGATG GGCACATCGC CCTTCATCTT GCTGTGAGGC GCTGTCAGAT GGAGGTCATC 
AAGACCCTCC TTGGCCATGG GTGCCTTGTG GATTTCCAGG ATCGGCATGG CAACACCCCC 
CTGCATGTGG CCTGCAAAGA TGGGAGCGCG CCCATCGTGG TGGCCCTCTG TGAAGCCAGC 
TGCAACCTGG ACATCTCAAA CAAGTATGGT CGGACTCCTC TCCACCTTGC AGCCAACAAC 
GGGATCCTAG ATGTGGTCCG CTACCTCTGC CTGATGGGCG CCAACGTGGA GGCTCTGACC 
TTGGATGGCA AGACGGCCGA GGACCTCGCC AAGGCAGAGC AGCACGAGCA CGTGGCAGGG 
CTCCTGGCAA GACTGCGGAA GGACACACAC CGAGGGCTCT TCATCCAGCA GCTCCGACCC 
ACGCAGAATC TCCAGCCCAG AATCAAGCTC AAACTGTTTG GCCACTCGGG ATCGGGGAAA 
TCCACACTGG TGGAATCTCT CAAGTGTGGG CTGTTAAGGA GTTTCTTCAG AAGGCGCCGG 
CCCAGACTGT CCTCTACCAA CTCCACCCGC TTCCCACCGT CGCCCCTGGC TACTAAGCCA 
ACAGTCTCAG TGAGCATTAA CAACCTGTAC CCCGGCTGTG AGAATGTGAG CGTGAGGAGC 
CGAAGCATGA TGTTTGAGCC AGGCCTCACC AAAGGGATGC TGGAAGTGTT CGTGGCTCCG 
TCTCACCACC TACACTGCTC GACCGATGAC CAGTCCACCA AAGCCATCGA CATCCAGAAT 
GCTTACTTGA ACGGAGTTGG TGATTTCAGC GTATGGGAGT TCTCTGGAAA CCCTGTGTAC 
TTCTGTTGCT ATGACTACTT CGCCGCCAAC GATCCCACAT CCATCCACAT CATCGTTTTC 
AGTCTCGAAG AACCCTATGA GATCCAGCTG AACCAAGTGA TTTTCTGGCT TAGTTTCTTG 
AAGTCTCTGG TCTCAGTTGA AGAACCCATA GCATTTGGAG GCAAGCTGAA GAACCCTCTC 
CGAGTTGTCC TGGTGGCCAC ACATGCTGAC ATCATGAACA TCCCTCGGCC TGCTGGGGGC 
GAGTTTGGAT ATGATAAAGA TACATCCTTG CTGAAAGAGA TCAGGAACAG GTTTGGGAAT 
GACCTTCATG TCTCAAATAA GCTGTTTGTG CTGGATGCAG GAGCATCTGG GTCTAAGGAC 
ATCAAGGTTC TCCGGAATCA CCTGCAAGAA ATACGGAGTC AGATTGTCTC GGGGTGCCCT 
CCCATGACTC ATCTGTGTGA GAAGATCATC TCCACTCTGC CCTCCTGGCG GAAGCTCAAC 
GGGCCAAACC AGCTGATGTC GCTCCAGCAG TTTGTGTATG ACGTGCAGGA CCAGCTGAAC 
CCCCTGGCCT CCGAGGATGA CCTCAGGCGC ATTGCACAGC AGCTGCACAG CACGGGAGAG 
ATCAACATCA TGCAGAGTGA GACAGTGCAG GATGTGTTGC TGCTGGATCC TCGATGGCTC 
TGCACCAACG TCCTGGGGAA GCTGCTGTCT GTGGAGACTC CCCGAGCCCT GCACCATTAT 
CGGGGCCGCT ACACCATGGA AGATATCCAG CGTCTGGTCC CCGACAGTGA CGTGGAGGAG 
CTGCTGCAGA TCCTGGATGC CATGGATATC TGCGCCCGAG ACCTGAGTAG CGGGACTATG 
GTGGATATCC CTGCTCTAAT CAAAACAGAC AGCCTGCAGC GCTCCTGGGC CGATGAGGAA 
GACGAGGTGA TGGTGTACGG AGGTGTGCGC ATCGTCCCTG TAGAGCACCT CACTCCCTTC 
CCCTGTGGCA TCTTTCACAA AGTTCAGGTC AACCTGTGCC GGTGGATCCA CCAGCAGAGC 
ACTGAGGGGG ACGCGGACAT CCGTCTGTGG GTGAGCGGCT GCAGGATCGC CAACCGTGGG 
GCTGAGTTGC TGGTGCTGCT GGTCAATCAC GGTCAGGGCA TTGAGGTACA GGTGCGTGGA 
CTGGAGACCG AGAAGATTAA GTGCTGCCTG CTGCTGGACT CGGTGTGCAG CACCATCGAG 
ACTGTCATGG CCACCACCTT GCCAGGGCTG TTGACGGTGA AGCACTACCT GAGCCCCCAG 
CAGCTAAGGG AGCATCACGA ACCAGTCATG GTCTACCAGC CCCGGGACTT CTTCCGTGCG 
CAGACCCTGA AAGAGAGCTC CCTCACCAAC ACCATGGGAG GGTACAAGGA GAGCTTCAGC 
AGCATCACGT GCTTTGGGTG TCACGATGTC TACTCACAGG CCAGTCTTGG CATGGACATC 
CATGCATCAG ACCTGAGTCT CCTGACCCGG AGGAAACTGA GTCGTCTTCT TGACCCACCG 
GACCCCATGG GGAAGGACTG GTGCCTTCTG GCCATGAACT TGGGCCTCCC GGACATGGTG 
GCCAAACACA ACGTCAATAC CAGGGCTTCT AGGGATTTCC TCCCCAGCCC AGTGCATGCC 
TTGCTACAGG AATGGACCTC CTACCCTGAG AGCACGGTGG GCATCCTTAT ATCCAAACTT 
CGGGAGCTGG GGCGCCGGGA TGCTGCGGAC TTTTTACTGA AGGCCTCCTC TGTGTTCAAG 
ATCAACCTTG ACGGCAATGG CCAGGAGGCC TATGCCTCAA GCTGTAACAG TGGCACATCC 
TACAATTCCA TAAGCTCAGT GGTGTCCCGG AGAGACTCCC ATGCTTGGAC CCCACTGTAT 
GATTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dapk1 ( XM_006253522.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006253584.1 Rattus norvegicus death associated protein kinase 1 (Dapk1), transcript variant X1, mRNA.

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CloneID	ORa37632
Clone ID Related Accession (Same CDS sequence)	XM_008771440.2 , XM_006253522.3 , XM_008771441.2
Accession Version	XM_006253522.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4329bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	death-associated protein kinase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005116.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006253522.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGTGT TCAGGCAGGA AAACGTGGAC GACTACTACG ACACCGGCGA GGAACTGGGC 
AGCGGACAGT TCGCCGTTGT GAAGAAATGT CGTGAGAAAA GTACCGGTCT TCAGTATGCG 
GCCAAGTTCA TCAAGAAAAG GAGGACCAAG TCCAGCCGGC GGGGCGTGAG CCGAGAGGAC 
ATCGAGCGGG AGGTCAGCAT CCTGAAGGAG ATCCGGCACC CAAACGTCAT CACCCTGCAT 
GAGGTCTACG AGAACAAGAC AGACGTCATT CTGATCCTGG AGCTTGTTGC AGGAGGCGAG 
CTGTTTGACT TCCTGGCTGA GAAGGAATCT CTCACTGAAG AAGAAGCAAC CGAGTTCCTC 
AAGCAGATCC TCAGCGGCGT TTACTACCTG CACTCGCTTC AAATCGCCCA CTTTGACCTG 
AAGCCGGAAA ACATAATGCT TCTGGATAGA AATGTGCCCA AACCTCGGAT CAAGATCATC 
GACTTCGGCC TGGCCCATAA AATTGACTTT GGAAATGAAT TCAAAAACAT ATTTGGGACA 
CCAGAGTTTG TGGCTCCGGA GATCGTCAAC TATGAGCCCC TTGGTCTTGA GGCAGACATG 
TGGAGCATTG GGGTAATAAC CTATATCCTC CTAAGCGGGG CCTCCCCTTT TCTTGGAGAC 
ACCAAGCAAG AAACATTAGC GAATGTATCT GCTGTCAACT ATGACTTTGA GGAGGAATTC 
TTCCGCAACA CCAGTACCCT TGCCAAAGAT TTCATCAGAA GGCTGCTGGT CAAGGATCCA 
AAGAAGAGGA TGACAATCCA GGACAGTTTG CAGCACCCCT GGATCAAGCC TAAAGACACC 
CAACAAGCAC TTAGTCGAAA AGCCTCAGCA GTAAACATGG AGAAATTCAA GAAGTTTGCA 
GCTCGGAAAA AATGGAAACA ATCTGTTCGC TTGATATCAC TGTGCCAAAG ATTATCCAGG 
TCATTTTTGT CCAGAAGTAA CATGAGCGTT GCCAGGAGCG ATGATACTCT GGATGAGGAA 
GACTCCTTTG TGATGAAAGC CATCATTCAT GCCATCAATG ATGACAACGT CCCCGGCCTG 
CAGCACCTCC TGGGCTCATT GTCCAGCTAT GATGTCAACC AGCCCAACAA GCACGGGACA 
CCTCCATTAC TGATTGCTGC AGGCTGTGGG AATATTCAGA TGTTACAGTT ACTCATAAAA 
CGGGGCTCAA GGATCGACGT CCAGGATAAG GGAGGATCCA ATGCTATCTA CTGGGCCTCT 
CGGCATGGCC ATGTGGATAC TTTGAAGTTT CTCAATGAGA ACAAATGCCC TTTGGATGTT 
AAAGACAAGT CTGGAGAGAC AGCTCTTCAC GTGGCAGCCC GCTATGGCCA TGCAGATGTG 
GTTCAACTCT TGTGCAGTTT TGGCTCTAAT CCTGATTTCC AGGACAAGGA AGAGGAAACC 
CCCCTGCACT GTGCTGCCTG GCACGGCTAT TACTCCGTGG CTAGAGCTCT TTGTGAAGTT 
GGCTGTAACG TGAATATCAA GAACCGGGAG GGAGAGACCC CACTGCTGAC AGCTTCTGCC 
AGGGGCTACC ATGACATTGT GGAGTGTCTG GCTGAACATG GAGCTGACCT GAATGCTTCT 
GACAAGGATG GGCACATCGC CCTTCATCTT GCTGTGAGGC GCTGTCAGAT GGAGGTCATC 
AAGACCCTCC TTGGCCATGG GTGCCTTGTG GATTTCCAGG ATCGGCATGG CAACACCCCC 
CTGCATGTGG CCTGCAAAGA TGGGAGCGCG CCCATCGTGG TGGCCCTCTG TGAAGCCAGC 
TGCAACCTGG ACATCTCAAA CAAGTATGGT CGGACTCCTC TCCACCTTGC AGCCAACAAC 
GGGATCCTAG ATGTGGTCCG CTACCTCTGC CTGATGGGCG CCAACGTGGA GGCTCTGACC 
TTGGATGGCA AGACGGCCGA GGACCTCGCC AAGGCAGAGC AGCACGAGCA CGTGGCAGGG 
CTCCTGGCAA GACTGCGGAA GGACACACAC CGAGGGCTCT TCATCCAGCA GCTCCGACCC 
ACGCAGAATC TCCAGCCCAG AATCAAGCTC AAACTGTTTG GCCACTCGGG ATCGGGGAAA 
TCCACACTGG TGGAATCTCT CAAGTGTGGG CTGTTAAGGA GTTTCTTCAG AAGGCGCCGG 
CCCAGACTGT CCTCTACCAA CTCCACCCGC TTCCCACCGT CGCCCCTGGC TACTAAGCCA 
ACAGTCTCAG TGAGCATTAA CAACCTGTAC CCCGGCTGTG AGAATGTGAG CGTGAGGAGC 
CGAAGCATGA TGTTTGAGCC AGGCCTCACC AAAGGGATGC TGGAAGTGTT CGTGGCTCCG 
TCTCACCACC TACACTGCTC GACCGATGAC CAGTCCACCA AAGCCATCGA CATCCAGAAT 
GCTTACTTGA ACGGAGTTGG TGATTTCAGC GTATGGGAGT TCTCTGGAAA CCCTGTGTAC 
TTCTGTTGCT ATGACTACTT CGCCGCCAAC GATCCCACAT CCATCCACAT CATCGTTTTC 
AGTCTCGAAG AACCCTATGA GATCCAGCTG AACCAAGTGA TTTTCTGGCT TAGTTTCTTG 
AAGTCTCTGG TCTCAGTTGA AGAACCCATA GCATTTGGAG GCAAGCTGAA GAACCCTCTC 
CGAGTTGTCC TGGTGGCCAC ACATGCTGAC ATCATGAACA TCCCTCGGCC TGCTGGGGGC 
GAGTTTGGAT ATGATAAAGA TACATCCTTG CTGAAAGAGA TCAGGAACAG GTTTGGGAAT 
GACCTTCATG TCTCAAATAA GCTGTTTGTG CTGGATGCAG GAGCATCTGG GTCTAAGGAC 
ATCAAGGTTC TCCGGAATCA CCTGCAAGAA ATACGGAGTC AGATTGTCTC GGGGTGCCCT 
CCCATGACTC ATCTGTGTGA GAAGATCATC TCCACTCTGC CCTCCTGGCG GAAGCTCAAC 
GGGCCAAACC AGCTGATGTC GCTCCAGCAG TTTGTGTATG ACGTGCAGGA CCAGCTGAAC 
CCCCTGGCCT CCGAGGATGA CCTCAGGCGC ATTGCACAGC AGCTGCACAG CACGGGAGAG 
ATCAACATCA TGCAGAGTGA GACAGTGCAG GATGTGTTGC TGCTGGATCC TCGATGGCTC 
TGCACCAACG TCCTGGGGAA GCTGCTGTCT GTGGAGACTC CCCGAGCCCT GCACCATTAT 
CGGGGCCGCT ACACCATGGA AGATATCCAG CGTCTGGTCC CCGACAGTGA CGTGGAGGAG 
CTGCTGCAGA TCCTGGATGC CATGGATATC TGCGCCCGAG ACCTGAGTAG CGGGACTATG 
GTGGATATCC CTGCTCTAAT CAAAACAGAC AGCCTGCAGC GCTCCTGGGC CGATGAGGAA 
GACGAGGTGA TGGTGTACGG AGGTGTGCGC ATCGTCCCTG TAGAGCACCT CACTCCCTTC 
CCCTGTGGCA TCTTTCACAA AGTTCAGGTC AACCTGTGCC GGTGGATCCA CCAGCAGAGC 
ACTGAGGGGG ACGCGGACAT CCGTCTGTGG GTGAGCGGCT GCAGGATCGC CAACCGTGGG 
GCTGAGTTGC TGGTGCTGCT GGTCAATCAC GGTCAGGGCA TTGAGGTACA GGTGCGTGGA 
CTGGAGACCG AGAAGATTAA GTGCTGCCTG CTGCTGGACT CGGTGTGCAG CACCATCGAG 
ACTGTCATGG CCACCACCTT GCCAGGGCTG TTGACGGTGA AGCACTACCT GAGCCCCCAG 
CAGCTAAGGG AGCATCACGA ACCAGTCATG GTCTACCAGC CCCGGGACTT CTTCCGTGCG 
CAGACCCTGA AAGAGAGCTC CCTCACCAAC ACCATGGGAG GGTACAAGGA GAGCTTCAGC 
AGCATCACGT GCTTTGGGTG TCACGATGTC TACTCACAGG CCAGTCTTGG CATGGACATC 
CATGCATCAG ACCTGAGTCT CCTGACCCGG AGGAAACTGA GTCGTCTTCT TGACCCACCG 
GACCCCATGG GGAAGGACTG GTGCCTTCTG GCCATGAACT TGGGCCTCCC GGACATGGTG 
GCCAAACACA ACGTCAATAC CAGGGCTTCT AGGGATTTCC TCCCCAGCCC AGTGCATGCC 
TTGCTACAGG AATGGACCTC CTACCCTGAG AGCACGGTGG GCATCCTTAT ATCCAAACTT 
CGGGAGCTGG GGCGCCGGGA TGCTGCGGAC TTTTTACTGA AGGCCTCCTC TGTGTTCAAG 
ATCAACCTTG ACGGCAATGG CCAGGAGGCC TATGCCTCAA GCTGTAACAG TGGCACATCC 
TACAATTCCA TAAGCTCAGT GGTGTCCCGG AGAGACTCCC ATGCTTGGAC CCCACTGTAT 
GATTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006253584.1
CDS	204..4532
Translation	MTVFRQENVDDYYDTGEELGSGQFAVVKKCREKSTGLQYAAKFIKKRRTKSSRRGVSREDIEREVSILKEIRHPNVITLHEVYENKTDVILILELVAGGELFDFLAEKESLTEEEATEFLKQILSGVYYLHSLQIAHFDLKPENIMLLDRNVPKPRIKIIDFGLAHKIDFGNEFKNIFGTPEFVAPEIVNYEPLGLEADMWSIGVITYILLSGASPFLGDTKQETLANVSAVNYDFEEEFFRNTSTLAKDFIRRLLVKDPKKRMTIQDSLQHPWIKPKDTQQALSRKASAVNMEKFKKFAARKKWKQSVRLISLCQRLSRSFLSRSNMSVARSDDTLDEEDSFVMKAIIHAINDDNVPGLQHLLGSLSSYDVNQPNKHGTPPLLIAAGCGNIQMLQLLIKRGSRIDVQDKGGSNAIYWASRHGHVDTLKFLNENKCPLDVKDKSGETALHVAARYGHADVVQLLCSFGSNPDFQDKEEETPLHCAAWHGYYSVARALCEVGCNVNIKNREGETPLLTASARGYHDIVECLAEHGADLNASDKDGHIALHLAVRRCQMEVIKTLLGHGCLVDFQDRHGNTPLHVACKDGSAPIVVALCEASCNLDISNKYGRTPLHLAANNGILDVVRYLCLMGANVEALTLDGKTAEDLAKAEQHEHVAGLLARLRKDTHRGLFIQQLRPTQNLQPRIKLKLFGHSGSGKSTLVESLKCGLLRSFFRRRRPRLSSTNSTRFPPSPLATKPTVSVSINNLYPGCENVSVRSRSMMFEPGLTKGMLEVFVAPSHHLHCSTDDQSTKAIDIQNAYLNGVGDFSVWEFSGNPVYFCCYDYFAANDPTSIHIIVFSLEEPYEIQLNQVIFWLSFLKSLVSVEEPIAFGGKLKNPLRVVLVATHADIMNIPRPAGGEFGYDKDTSLLKEIRNRFGNDLHVSNKLFVLDAGASGSKDIKVLRNHLQEIRSQIVSGCPPMTHLCEKIISTLPSWRKLNGPNQLMSLQQFVYDVQDQLNPLASEDDLRRIAQQLHSTGEINIMQSETVQDVLLLDPRWLCTNVLGKLLSVETPRALHHYRGRYTMEDIQRLVPDSDVEELLQILDAMDICARDLSSGTMVDIPALIKTDSLQRSWADEEDEVMVYGGVRIVPVEHLTPFPCGIFHKVQVNLCRWIHQQSTEGDADIRLWVSGCRIANRGAELLVLLVNHGQGIEVQVRGLETEKIKCCLLLDSVCSTIETVMATTLPGLLTVKHYLSPQQLREHHEPVMVYQPRDFFRAQTLKESSLTNTMGGYKESFSSITCFGCHDVYSQASLGMDIHASDLSLLTRRKLSRLLDPPDPMGKDWCLLAMNLGLPDMVAKHNVNTRASRDFLPSPVHALLQEWTSYPESTVGILISKLRELGRRDAADFLLKASSVFKINLDGNGQEAYASSCNSGTSYNSISSVVSRRDSHAWTPLYDL

Target ORF information:

RefSeq Version	XM_006253522.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus death associated protein kinase 1 (Dapk1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006253522.3

ORF Insert Sequence:

ATGACTGTGT TCAGGCAGGA AAACGTGGAC GACTACTACG ACACCGGCGA GGAACTGGGC 
AGCGGACAGT TCGCCGTTGT GAAGAAATGT CGTGAGAAAA GTACCGGTCT TCAGTATGCG 
GCCAAGTTCA TCAAGAAAAG GAGGACCAAG TCCAGCCGGC GGGGCGTGAG CCGAGAGGAC 
ATCGAGCGGG AGGTCAGCAT CCTGAAGGAG ATCCGGCACC CAAACGTCAT CACCCTGCAT 
GAGGTCTACG AGAACAAGAC AGACGTCATT CTGATCCTGG AGCTTGTTGC AGGAGGCGAG 
CTGTTTGACT TCCTGGCTGA GAAGGAATCT CTCACTGAAG AAGAAGCAAC CGAGTTCCTC 
AAGCAGATCC TCAGCGGCGT TTACTACCTG CACTCGCTTC AAATCGCCCA CTTTGACCTG 
AAGCCGGAAA ACATAATGCT TCTGGATAGA AATGTGCCCA AACCTCGGAT CAAGATCATC 
GACTTCGGCC TGGCCCATAA AATTGACTTT GGAAATGAAT TCAAAAACAT ATTTGGGACA 
CCAGAGTTTG TGGCTCCGGA GATCGTCAAC TATGAGCCCC TTGGTCTTGA GGCAGACATG 
TGGAGCATTG GGGTAATAAC CTATATCCTC CTAAGCGGGG CCTCCCCTTT TCTTGGAGAC 
ACCAAGCAAG AAACATTAGC GAATGTATCT GCTGTCAACT ATGACTTTGA GGAGGAATTC 
TTCCGCAACA CCAGTACCCT TGCCAAAGAT TTCATCAGAA GGCTGCTGGT CAAGGATCCA 
AAGAAGAGGA TGACAATCCA GGACAGTTTG CAGCACCCCT GGATCAAGCC TAAAGACACC 
CAACAAGCAC TTAGTCGAAA AGCCTCAGCA GTAAACATGG AGAAATTCAA GAAGTTTGCA 
GCTCGGAAAA AATGGAAACA ATCTGTTCGC TTGATATCAC TGTGCCAAAG ATTATCCAGG 
TCATTTTTGT CCAGAAGTAA CATGAGCGTT GCCAGGAGCG ATGATACTCT GGATGAGGAA 
GACTCCTTTG TGATGAAAGC CATCATTCAT GCCATCAATG ATGACAACGT CCCCGGCCTG 
CAGCACCTCC TGGGCTCATT GTCCAGCTAT GATGTCAACC AGCCCAACAA GCACGGGACA 
CCTCCATTAC TGATTGCTGC AGGCTGTGGG AATATTCAGA TGTTACAGTT ACTCATAAAA 
CGGGGCTCAA GGATCGACGT CCAGGATAAG GGAGGATCCA ATGCTATCTA CTGGGCCTCT 
CGGCATGGCC ATGTGGATAC TTTGAAGTTT CTCAATGAGA ACAAATGCCC TTTGGATGTT 
AAAGACAAGT CTGGAGAGAC AGCTCTTCAC GTGGCAGCCC GCTATGGCCA TGCAGATGTG 
GTTCAACTCT TGTGCAGTTT TGGCTCTAAT CCTGATTTCC AGGACAAGGA AGAGGAAACC 
CCCCTGCACT GTGCTGCCTG GCACGGCTAT TACTCCGTGG CTAGAGCTCT TTGTGAAGTT 
GGCTGTAACG TGAATATCAA GAACCGGGAG GGAGAGACCC CACTGCTGAC AGCTTCTGCC 
AGGGGCTACC ATGACATTGT GGAGTGTCTG GCTGAACATG GAGCTGACCT GAATGCTTCT 
GACAAGGATG GGCACATCGC CCTTCATCTT GCTGTGAGGC GCTGTCAGAT GGAGGTCATC 
AAGACCCTCC TTGGCCATGG GTGCCTTGTG GATTTCCAGG ATCGGCATGG CAACACCCCC 
CTGCATGTGG CCTGCAAAGA TGGGAGCGCG CCCATCGTGG TGGCCCTCTG TGAAGCCAGC 
TGCAACCTGG ACATCTCAAA CAAGTATGGT CGGACTCCTC TCCACCTTGC AGCCAACAAC 
GGGATCCTAG ATGTGGTCCG CTACCTCTGC CTGATGGGCG CCAACGTGGA GGCTCTGACC 
TTGGATGGCA AGACGGCCGA GGACCTCGCC AAGGCAGAGC AGCACGAGCA CGTGGCAGGG 
CTCCTGGCAA GACTGCGGAA GGACACACAC CGAGGGCTCT TCATCCAGCA GCTCCGACCC 
ACGCAGAATC TCCAGCCCAG AATCAAGCTC AAACTGTTTG GCCACTCGGG ATCGGGGAAA 
TCCACACTGG TGGAATCTCT CAAGTGTGGG CTGTTAAGGA GTTTCTTCAG AAGGCGCCGG 
CCCAGACTGT CCTCTACCAA CTCCACCCGC TTCCCACCGT CGCCCCTGGC TACTAAGCCA 
ACAGTCTCAG TGAGCATTAA CAACCTGTAC CCCGGCTGTG AGAATGTGAG CGTGAGGAGC 
CGAAGCATGA TGTTTGAGCC AGGCCTCACC AAAGGGATGC TGGAAGTGTT CGTGGCTCCG 
TCTCACCACC TACACTGCTC GACCGATGAC CAGTCCACCA AAGCCATCGA CATCCAGAAT 
GCTTACTTGA ACGGAGTTGG TGATTTCAGC GTATGGGAGT TCTCTGGAAA CCCTGTGTAC 
TTCTGTTGCT ATGACTACTT CGCCGCCAAC GATCCCACAT CCATCCACAT CATCGTTTTC 
AGTCTCGAAG AACCCTATGA GATCCAGCTG AACCAAGTGA TTTTCTGGCT TAGTTTCTTG 
AAGTCTCTGG TCTCAGTTGA AGAACCCATA GCATTTGGAG GCAAGCTGAA GAACCCTCTC 
CGAGTTGTCC TGGTGGCCAC ACATGCTGAC ATCATGAACA TCCCTCGGCC TGCTGGGGGC 
GAGTTTGGAT ATGATAAAGA TACATCCTTG CTGAAAGAGA TCAGGAACAG GTTTGGGAAT 
GACCTTCATG TCTCAAATAA GCTGTTTGTG CTGGATGCAG GAGCATCTGG GTCTAAGGAC 
ATCAAGGTTC TCCGGAATCA CCTGCAAGAA ATACGGAGTC AGATTGTCTC GGGGTGCCCT 
CCCATGACTC ATCTGTGTGA GAAGATCATC TCCACTCTGC CCTCCTGGCG GAAGCTCAAC 
GGGCCAAACC AGCTGATGTC GCTCCAGCAG TTTGTGTATG ACGTGCAGGA CCAGCTGAAC 
CCCCTGGCCT CCGAGGATGA CCTCAGGCGC ATTGCACAGC AGCTGCACAG CACGGGAGAG 
ATCAACATCA TGCAGAGTGA GACAGTGCAG GATGTGTTGC TGCTGGATCC TCGATGGCTC 
TGCACCAACG TCCTGGGGAA GCTGCTGTCT GTGGAGACTC CCCGAGCCCT GCACCATTAT 
CGGGGCCGCT ACACCATGGA AGATATCCAG CGTCTGGTCC CCGACAGTGA CGTGGAGGAG 
CTGCTGCAGA TCCTGGATGC CATGGATATC TGCGCCCGAG ACCTGAGTAG CGGGACTATG 
GTGGATATCC CTGCTCTAAT CAAAACAGAC AGCCTGCAGC GCTCCTGGGC CGATGAGGAA 
GACGAGGTGA TGGTGTACGG AGGTGTGCGC ATCGTCCCTG TAGAGCACCT CACTCCCTTC 
CCCTGTGGCA TCTTTCACAA AGTTCAGGTC AACCTGTGCC GGTGGATCCA CCAGCAGAGC 
ACTGAGGGGG ACGCGGACAT CCGTCTGTGG GTGAGCGGCT GCAGGATCGC CAACCGTGGG 
GCTGAGTTGC TGGTGCTGCT GGTCAATCAC GGTCAGGGCA TTGAGGTACA GGTGCGTGGA 
CTGGAGACCG AGAAGATTAA GTGCTGCCTG CTGCTGGACT CGGTGTGCAG CACCATCGAG 
ACTGTCATGG CCACCACCTT GCCAGGGCTG TTGACGGTGA AGCACTACCT GAGCCCCCAG 
CAGCTAAGGG AGCATCACGA ACCAGTCATG GTCTACCAGC CCCGGGACTT CTTCCGTGCG 
CAGACCCTGA AAGAGAGCTC CCTCACCAAC ACCATGGGAG GGTACAAGGA GAGCTTCAGC 
AGCATCACGT GCTTTGGGTG TCACGATGTC TACTCACAGG CCAGTCTTGG CATGGACATC 
CATGCATCAG ACCTGAGTCT CCTGACCCGG AGGAAACTGA GTCGTCTTCT TGACCCACCG 
GACCCCATGG GGAAGGACTG GTGCCTTCTG GCCATGAACT TGGGCCTCCC GGACATGGTG 
GCCAAACACA ACGTCAATAC CAGGGCTTCT AGGGATTTCC TCCCCAGCCC AGTGCATGCC 
TTGCTACAGG AATGGACCTC CTACCCTGAG AGCACGGTGG GCATCCTTAT ATCCAAACTT 
CGGGAGCTGG GGCGCCGGGA TGCTGCGGAC TTTTTACTGA AGGCCTCCTC TGTGTTCAAG 
ATCAACCTTG ACGGCAATGG CCAGGAGGCC TATGCCTCAA GCTGTAACAG TGGCACATCC 
TACAATTCCA TAAGCTCAGT GGTGTCCCGG AGAGACTCCC ATGCTTGGAC CCCACTGTAT 
GATTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dapk1 ( XM_008771441.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008769663.1 Rattus norvegicus death associated protein kinase 1 (Dapk1), transcript variant X3, mRNA.

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CloneID	ORa37632
Clone ID Related Accession (Same CDS sequence)	XM_008771440.2 , XM_006253522.3 , XM_008771441.2
Accession Version	XM_008771441.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4329bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	death-associated protein kinase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005116.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008771441.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGTGT TCAGGCAGGA AAACGTGGAC GACTACTACG ACACCGGCGA GGAACTGGGC 
AGCGGACAGT TCGCCGTTGT GAAGAAATGT CGTGAGAAAA GTACCGGTCT TCAGTATGCG 
GCCAAGTTCA TCAAGAAAAG GAGGACCAAG TCCAGCCGGC GGGGCGTGAG CCGAGAGGAC 
ATCGAGCGGG AGGTCAGCAT CCTGAAGGAG ATCCGGCACC CAAACGTCAT CACCCTGCAT 
GAGGTCTACG AGAACAAGAC AGACGTCATT CTGATCCTGG AGCTTGTTGC AGGAGGCGAG 
CTGTTTGACT TCCTGGCTGA GAAGGAATCT CTCACTGAAG AAGAAGCAAC CGAGTTCCTC 
AAGCAGATCC TCAGCGGCGT TTACTACCTG CACTCGCTTC AAATCGCCCA CTTTGACCTG 
AAGCCGGAAA ACATAATGCT TCTGGATAGA AATGTGCCCA AACCTCGGAT CAAGATCATC 
GACTTCGGCC TGGCCCATAA AATTGACTTT GGAAATGAAT TCAAAAACAT ATTTGGGACA 
CCAGAGTTTG TGGCTCCGGA GATCGTCAAC TATGAGCCCC TTGGTCTTGA GGCAGACATG 
TGGAGCATTG GGGTAATAAC CTATATCCTC CTAAGCGGGG CCTCCCCTTT TCTTGGAGAC 
ACCAAGCAAG AAACATTAGC GAATGTATCT GCTGTCAACT ATGACTTTGA GGAGGAATTC 
TTCCGCAACA CCAGTACCCT TGCCAAAGAT TTCATCAGAA GGCTGCTGGT CAAGGATCCA 
AAGAAGAGGA TGACAATCCA GGACAGTTTG CAGCACCCCT GGATCAAGCC TAAAGACACC 
CAACAAGCAC TTAGTCGAAA AGCCTCAGCA GTAAACATGG AGAAATTCAA GAAGTTTGCA 
GCTCGGAAAA AATGGAAACA ATCTGTTCGC TTGATATCAC TGTGCCAAAG ATTATCCAGG 
TCATTTTTGT CCAGAAGTAA CATGAGCGTT GCCAGGAGCG ATGATACTCT GGATGAGGAA 
GACTCCTTTG TGATGAAAGC CATCATTCAT GCCATCAATG ATGACAACGT CCCCGGCCTG 
CAGCACCTCC TGGGCTCATT GTCCAGCTAT GATGTCAACC AGCCCAACAA GCACGGGACA 
CCTCCATTAC TGATTGCTGC AGGCTGTGGG AATATTCAGA TGTTACAGTT ACTCATAAAA 
CGGGGCTCAA GGATCGACGT CCAGGATAAG GGAGGATCCA ATGCTATCTA CTGGGCCTCT 
CGGCATGGCC ATGTGGATAC TTTGAAGTTT CTCAATGAGA ACAAATGCCC TTTGGATGTT 
AAAGACAAGT CTGGAGAGAC AGCTCTTCAC GTGGCAGCCC GCTATGGCCA TGCAGATGTG 
GTTCAACTCT TGTGCAGTTT TGGCTCTAAT CCTGATTTCC AGGACAAGGA AGAGGAAACC 
CCCCTGCACT GTGCTGCCTG GCACGGCTAT TACTCCGTGG CTAGAGCTCT TTGTGAAGTT 
GGCTGTAACG TGAATATCAA GAACCGGGAG GGAGAGACCC CACTGCTGAC AGCTTCTGCC 
AGGGGCTACC ATGACATTGT GGAGTGTCTG GCTGAACATG GAGCTGACCT GAATGCTTCT 
GACAAGGATG GGCACATCGC CCTTCATCTT GCTGTGAGGC GCTGTCAGAT GGAGGTCATC 
AAGACCCTCC TTGGCCATGG GTGCCTTGTG GATTTCCAGG ATCGGCATGG CAACACCCCC 
CTGCATGTGG CCTGCAAAGA TGGGAGCGCG CCCATCGTGG TGGCCCTCTG TGAAGCCAGC 
TGCAACCTGG ACATCTCAAA CAAGTATGGT CGGACTCCTC TCCACCTTGC AGCCAACAAC 
GGGATCCTAG ATGTGGTCCG CTACCTCTGC CTGATGGGCG CCAACGTGGA GGCTCTGACC 
TTGGATGGCA AGACGGCCGA GGACCTCGCC AAGGCAGAGC AGCACGAGCA CGTGGCAGGG 
CTCCTGGCAA GACTGCGGAA GGACACACAC CGAGGGCTCT TCATCCAGCA GCTCCGACCC 
ACGCAGAATC TCCAGCCCAG AATCAAGCTC AAACTGTTTG GCCACTCGGG ATCGGGGAAA 
TCCACACTGG TGGAATCTCT CAAGTGTGGG CTGTTAAGGA GTTTCTTCAG AAGGCGCCGG 
CCCAGACTGT CCTCTACCAA CTCCACCCGC TTCCCACCGT CGCCCCTGGC TACTAAGCCA 
ACAGTCTCAG TGAGCATTAA CAACCTGTAC CCCGGCTGTG AGAATGTGAG CGTGAGGAGC 
CGAAGCATGA TGTTTGAGCC AGGCCTCACC AAAGGGATGC TGGAAGTGTT CGTGGCTCCG 
TCTCACCACC TACACTGCTC GACCGATGAC CAGTCCACCA AAGCCATCGA CATCCAGAAT 
GCTTACTTGA ACGGAGTTGG TGATTTCAGC GTATGGGAGT TCTCTGGAAA CCCTGTGTAC 
TTCTGTTGCT ATGACTACTT CGCCGCCAAC GATCCCACAT CCATCCACAT CATCGTTTTC 
AGTCTCGAAG AACCCTATGA GATCCAGCTG AACCAAGTGA TTTTCTGGCT TAGTTTCTTG 
AAGTCTCTGG TCTCAGTTGA AGAACCCATA GCATTTGGAG GCAAGCTGAA GAACCCTCTC 
CGAGTTGTCC TGGTGGCCAC ACATGCTGAC ATCATGAACA TCCCTCGGCC TGCTGGGGGC 
GAGTTTGGAT ATGATAAAGA TACATCCTTG CTGAAAGAGA TCAGGAACAG GTTTGGGAAT 
GACCTTCATG TCTCAAATAA GCTGTTTGTG CTGGATGCAG GAGCATCTGG GTCTAAGGAC 
ATCAAGGTTC TCCGGAATCA CCTGCAAGAA ATACGGAGTC AGATTGTCTC GGGGTGCCCT 
CCCATGACTC ATCTGTGTGA GAAGATCATC TCCACTCTGC CCTCCTGGCG GAAGCTCAAC 
GGGCCAAACC AGCTGATGTC GCTCCAGCAG TTTGTGTATG ACGTGCAGGA CCAGCTGAAC 
CCCCTGGCCT CCGAGGATGA CCTCAGGCGC ATTGCACAGC AGCTGCACAG CACGGGAGAG 
ATCAACATCA TGCAGAGTGA GACAGTGCAG GATGTGTTGC TGCTGGATCC TCGATGGCTC 
TGCACCAACG TCCTGGGGAA GCTGCTGTCT GTGGAGACTC CCCGAGCCCT GCACCATTAT 
CGGGGCCGCT ACACCATGGA AGATATCCAG CGTCTGGTCC CCGACAGTGA CGTGGAGGAG 
CTGCTGCAGA TCCTGGATGC CATGGATATC TGCGCCCGAG ACCTGAGTAG CGGGACTATG 
GTGGATATCC CTGCTCTAAT CAAAACAGAC AGCCTGCAGC GCTCCTGGGC CGATGAGGAA 
GACGAGGTGA TGGTGTACGG AGGTGTGCGC ATCGTCCCTG TAGAGCACCT CACTCCCTTC 
CCCTGTGGCA TCTTTCACAA AGTTCAGGTC AACCTGTGCC GGTGGATCCA CCAGCAGAGC 
ACTGAGGGGG ACGCGGACAT CCGTCTGTGG GTGAGCGGCT GCAGGATCGC CAACCGTGGG 
GCTGAGTTGC TGGTGCTGCT GGTCAATCAC GGTCAGGGCA TTGAGGTACA GGTGCGTGGA 
CTGGAGACCG AGAAGATTAA GTGCTGCCTG CTGCTGGACT CGGTGTGCAG CACCATCGAG 
ACTGTCATGG CCACCACCTT GCCAGGGCTG TTGACGGTGA AGCACTACCT GAGCCCCCAG 
CAGCTAAGGG AGCATCACGA ACCAGTCATG GTCTACCAGC CCCGGGACTT CTTCCGTGCG 
CAGACCCTGA AAGAGAGCTC CCTCACCAAC ACCATGGGAG GGTACAAGGA GAGCTTCAGC 
AGCATCACGT GCTTTGGGTG TCACGATGTC TACTCACAGG CCAGTCTTGG CATGGACATC 
CATGCATCAG ACCTGAGTCT CCTGACCCGG AGGAAACTGA GTCGTCTTCT TGACCCACCG 
GACCCCATGG GGAAGGACTG GTGCCTTCTG GCCATGAACT TGGGCCTCCC GGACATGGTG 
GCCAAACACA ACGTCAATAC CAGGGCTTCT AGGGATTTCC TCCCCAGCCC AGTGCATGCC 
TTGCTACAGG AATGGACCTC CTACCCTGAG AGCACGGTGG GCATCCTTAT ATCCAAACTT 
CGGGAGCTGG GGCGCCGGGA TGCTGCGGAC TTTTTACTGA AGGCCTCCTC TGTGTTCAAG 
ATCAACCTTG ACGGCAATGG CCAGGAGGCC TATGCCTCAA GCTGTAACAG TGGCACATCC 
TACAATTCCA TAAGCTCAGT GGTGTCCCGG AGAGACTCCC ATGCTTGGAC CCCACTGTAT 
GATTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008769663.1
CDS	200..4528
Translation	MTVFRQENVDDYYDTGEELGSGQFAVVKKCREKSTGLQYAAKFIKKRRTKSSRRGVSREDIEREVSILKEIRHPNVITLHEVYENKTDVILILELVAGGELFDFLAEKESLTEEEATEFLKQILSGVYYLHSLQIAHFDLKPENIMLLDRNVPKPRIKIIDFGLAHKIDFGNEFKNIFGTPEFVAPEIVNYEPLGLEADMWSIGVITYILLSGASPFLGDTKQETLANVSAVNYDFEEEFFRNTSTLAKDFIRRLLVKDPKKRMTIQDSLQHPWIKPKDTQQALSRKASAVNMEKFKKFAARKKWKQSVRLISLCQRLSRSFLSRSNMSVARSDDTLDEEDSFVMKAIIHAINDDNVPGLQHLLGSLSSYDVNQPNKHGTPPLLIAAGCGNIQMLQLLIKRGSRIDVQDKGGSNAIYWASRHGHVDTLKFLNENKCPLDVKDKSGETALHVAARYGHADVVQLLCSFGSNPDFQDKEEETPLHCAAWHGYYSVARALCEVGCNVNIKNREGETPLLTASARGYHDIVECLAEHGADLNASDKDGHIALHLAVRRCQMEVIKTLLGHGCLVDFQDRHGNTPLHVACKDGSAPIVVALCEASCNLDISNKYGRTPLHLAANNGILDVVRYLCLMGANVEALTLDGKTAEDLAKAEQHEHVAGLLARLRKDTHRGLFIQQLRPTQNLQPRIKLKLFGHSGSGKSTLVESLKCGLLRSFFRRRRPRLSSTNSTRFPPSPLATKPTVSVSINNLYPGCENVSVRSRSMMFEPGLTKGMLEVFVAPSHHLHCSTDDQSTKAIDIQNAYLNGVGDFSVWEFSGNPVYFCCYDYFAANDPTSIHIIVFSLEEPYEIQLNQVIFWLSFLKSLVSVEEPIAFGGKLKNPLRVVLVATHADIMNIPRPAGGEFGYDKDTSLLKEIRNRFGNDLHVSNKLFVLDAGASGSKDIKVLRNHLQEIRSQIVSGCPPMTHLCEKIISTLPSWRKLNGPNQLMSLQQFVYDVQDQLNPLASEDDLRRIAQQLHSTGEINIMQSETVQDVLLLDPRWLCTNVLGKLLSVETPRALHHYRGRYTMEDIQRLVPDSDVEELLQILDAMDICARDLSSGTMVDIPALIKTDSLQRSWADEEDEVMVYGGVRIVPVEHLTPFPCGIFHKVQVNLCRWIHQQSTEGDADIRLWVSGCRIANRGAELLVLLVNHGQGIEVQVRGLETEKIKCCLLLDSVCSTIETVMATTLPGLLTVKHYLSPQQLREHHEPVMVYQPRDFFRAQTLKESSLTNTMGGYKESFSSITCFGCHDVYSQASLGMDIHASDLSLLTRRKLSRLLDPPDPMGKDWCLLAMNLGLPDMVAKHNVNTRASRDFLPSPVHALLQEWTSYPESTVGILISKLRELGRRDAADFLLKASSVFKINLDGNGQEAYASSCNSGTSYNSISSVVSRRDSHAWTPLYDL

Target ORF information:

RefSeq Version	XM_008771441.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus death associated protein kinase 1 (Dapk1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008771441.2

ORF Insert Sequence:

ATGACTGTGT TCAGGCAGGA AAACGTGGAC GACTACTACG ACACCGGCGA GGAACTGGGC 
AGCGGACAGT TCGCCGTTGT GAAGAAATGT CGTGAGAAAA GTACCGGTCT TCAGTATGCG 
GCCAAGTTCA TCAAGAAAAG GAGGACCAAG TCCAGCCGGC GGGGCGTGAG CCGAGAGGAC 
ATCGAGCGGG AGGTCAGCAT CCTGAAGGAG ATCCGGCACC CAAACGTCAT CACCCTGCAT 
GAGGTCTACG AGAACAAGAC AGACGTCATT CTGATCCTGG AGCTTGTTGC AGGAGGCGAG 
CTGTTTGACT TCCTGGCTGA GAAGGAATCT CTCACTGAAG AAGAAGCAAC CGAGTTCCTC 
AAGCAGATCC TCAGCGGCGT TTACTACCTG CACTCGCTTC AAATCGCCCA CTTTGACCTG 
AAGCCGGAAA ACATAATGCT TCTGGATAGA AATGTGCCCA AACCTCGGAT CAAGATCATC 
GACTTCGGCC TGGCCCATAA AATTGACTTT GGAAATGAAT TCAAAAACAT ATTTGGGACA 
CCAGAGTTTG TGGCTCCGGA GATCGTCAAC TATGAGCCCC TTGGTCTTGA GGCAGACATG 
TGGAGCATTG GGGTAATAAC CTATATCCTC CTAAGCGGGG CCTCCCCTTT TCTTGGAGAC 
ACCAAGCAAG AAACATTAGC GAATGTATCT GCTGTCAACT ATGACTTTGA GGAGGAATTC 
TTCCGCAACA CCAGTACCCT TGCCAAAGAT TTCATCAGAA GGCTGCTGGT CAAGGATCCA 
AAGAAGAGGA TGACAATCCA GGACAGTTTG CAGCACCCCT GGATCAAGCC TAAAGACACC 
CAACAAGCAC TTAGTCGAAA AGCCTCAGCA GTAAACATGG AGAAATTCAA GAAGTTTGCA 
GCTCGGAAAA AATGGAAACA ATCTGTTCGC TTGATATCAC TGTGCCAAAG ATTATCCAGG 
TCATTTTTGT CCAGAAGTAA CATGAGCGTT GCCAGGAGCG ATGATACTCT GGATGAGGAA 
GACTCCTTTG TGATGAAAGC CATCATTCAT GCCATCAATG ATGACAACGT CCCCGGCCTG 
CAGCACCTCC TGGGCTCATT GTCCAGCTAT GATGTCAACC AGCCCAACAA GCACGGGACA 
CCTCCATTAC TGATTGCTGC AGGCTGTGGG AATATTCAGA TGTTACAGTT ACTCATAAAA 
CGGGGCTCAA GGATCGACGT CCAGGATAAG GGAGGATCCA ATGCTATCTA CTGGGCCTCT 
CGGCATGGCC ATGTGGATAC TTTGAAGTTT CTCAATGAGA ACAAATGCCC TTTGGATGTT 
AAAGACAAGT CTGGAGAGAC AGCTCTTCAC GTGGCAGCCC GCTATGGCCA TGCAGATGTG 
GTTCAACTCT TGTGCAGTTT TGGCTCTAAT CCTGATTTCC AGGACAAGGA AGAGGAAACC 
CCCCTGCACT GTGCTGCCTG GCACGGCTAT TACTCCGTGG CTAGAGCTCT TTGTGAAGTT 
GGCTGTAACG TGAATATCAA GAACCGGGAG GGAGAGACCC CACTGCTGAC AGCTTCTGCC 
AGGGGCTACC ATGACATTGT GGAGTGTCTG GCTGAACATG GAGCTGACCT GAATGCTTCT 
GACAAGGATG GGCACATCGC CCTTCATCTT GCTGTGAGGC GCTGTCAGAT GGAGGTCATC 
AAGACCCTCC TTGGCCATGG GTGCCTTGTG GATTTCCAGG ATCGGCATGG CAACACCCCC 
CTGCATGTGG CCTGCAAAGA TGGGAGCGCG CCCATCGTGG TGGCCCTCTG TGAAGCCAGC 
TGCAACCTGG ACATCTCAAA CAAGTATGGT CGGACTCCTC TCCACCTTGC AGCCAACAAC 
GGGATCCTAG ATGTGGTCCG CTACCTCTGC CTGATGGGCG CCAACGTGGA GGCTCTGACC 
TTGGATGGCA AGACGGCCGA GGACCTCGCC AAGGCAGAGC AGCACGAGCA CGTGGCAGGG 
CTCCTGGCAA GACTGCGGAA GGACACACAC CGAGGGCTCT TCATCCAGCA GCTCCGACCC 
ACGCAGAATC TCCAGCCCAG AATCAAGCTC AAACTGTTTG GCCACTCGGG ATCGGGGAAA 
TCCACACTGG TGGAATCTCT CAAGTGTGGG CTGTTAAGGA GTTTCTTCAG AAGGCGCCGG 
CCCAGACTGT CCTCTACCAA CTCCACCCGC TTCCCACCGT CGCCCCTGGC TACTAAGCCA 
ACAGTCTCAG TGAGCATTAA CAACCTGTAC CCCGGCTGTG AGAATGTGAG CGTGAGGAGC 
CGAAGCATGA TGTTTGAGCC AGGCCTCACC AAAGGGATGC TGGAAGTGTT CGTGGCTCCG 
TCTCACCACC TACACTGCTC GACCGATGAC CAGTCCACCA AAGCCATCGA CATCCAGAAT 
GCTTACTTGA ACGGAGTTGG TGATTTCAGC GTATGGGAGT TCTCTGGAAA CCCTGTGTAC 
TTCTGTTGCT ATGACTACTT CGCCGCCAAC GATCCCACAT CCATCCACAT CATCGTTTTC 
AGTCTCGAAG AACCCTATGA GATCCAGCTG AACCAAGTGA TTTTCTGGCT TAGTTTCTTG 
AAGTCTCTGG TCTCAGTTGA AGAACCCATA GCATTTGGAG GCAAGCTGAA GAACCCTCTC 
CGAGTTGTCC TGGTGGCCAC ACATGCTGAC ATCATGAACA TCCCTCGGCC TGCTGGGGGC 
GAGTTTGGAT ATGATAAAGA TACATCCTTG CTGAAAGAGA TCAGGAACAG GTTTGGGAAT 
GACCTTCATG TCTCAAATAA GCTGTTTGTG CTGGATGCAG GAGCATCTGG GTCTAAGGAC 
ATCAAGGTTC TCCGGAATCA CCTGCAAGAA ATACGGAGTC AGATTGTCTC GGGGTGCCCT 
CCCATGACTC ATCTGTGTGA GAAGATCATC TCCACTCTGC CCTCCTGGCG GAAGCTCAAC 
GGGCCAAACC AGCTGATGTC GCTCCAGCAG TTTGTGTATG ACGTGCAGGA CCAGCTGAAC 
CCCCTGGCCT CCGAGGATGA CCTCAGGCGC ATTGCACAGC AGCTGCACAG CACGGGAGAG 
ATCAACATCA TGCAGAGTGA GACAGTGCAG GATGTGTTGC TGCTGGATCC TCGATGGCTC 
TGCACCAACG TCCTGGGGAA GCTGCTGTCT GTGGAGACTC CCCGAGCCCT GCACCATTAT 
CGGGGCCGCT ACACCATGGA AGATATCCAG CGTCTGGTCC CCGACAGTGA CGTGGAGGAG 
CTGCTGCAGA TCCTGGATGC CATGGATATC TGCGCCCGAG ACCTGAGTAG CGGGACTATG 
GTGGATATCC CTGCTCTAAT CAAAACAGAC AGCCTGCAGC GCTCCTGGGC CGATGAGGAA 
GACGAGGTGA TGGTGTACGG AGGTGTGCGC ATCGTCCCTG TAGAGCACCT CACTCCCTTC 
CCCTGTGGCA TCTTTCACAA AGTTCAGGTC AACCTGTGCC GGTGGATCCA CCAGCAGAGC 
ACTGAGGGGG ACGCGGACAT CCGTCTGTGG GTGAGCGGCT GCAGGATCGC CAACCGTGGG 
GCTGAGTTGC TGGTGCTGCT GGTCAATCAC GGTCAGGGCA TTGAGGTACA GGTGCGTGGA 
CTGGAGACCG AGAAGATTAA GTGCTGCCTG CTGCTGGACT CGGTGTGCAG CACCATCGAG 
ACTGTCATGG CCACCACCTT GCCAGGGCTG TTGACGGTGA AGCACTACCT GAGCCCCCAG 
CAGCTAAGGG AGCATCACGA ACCAGTCATG GTCTACCAGC CCCGGGACTT CTTCCGTGCG 
CAGACCCTGA AAGAGAGCTC CCTCACCAAC ACCATGGGAG GGTACAAGGA GAGCTTCAGC 
AGCATCACGT GCTTTGGGTG TCACGATGTC TACTCACAGG CCAGTCTTGG CATGGACATC 
CATGCATCAG ACCTGAGTCT CCTGACCCGG AGGAAACTGA GTCGTCTTCT TGACCCACCG 
GACCCCATGG GGAAGGACTG GTGCCTTCTG GCCATGAACT TGGGCCTCCC GGACATGGTG 
GCCAAACACA ACGTCAATAC CAGGGCTTCT AGGGATTTCC TCCCCAGCCC AGTGCATGCC 
TTGCTACAGG AATGGACCTC CTACCCTGAG AGCACGGTGG GCATCCTTAT ATCCAAACTT 
CGGGAGCTGG GGCGCCGGGA TGCTGCGGAC TTTTTACTGA AGGCCTCCTC TGTGTTCAAG 
ATCAACCTTG ACGGCAATGG CCAGGAGGCC TATGCCTCAA GCTGTAACAG TGGCACATCC 
TACAATTCCA TAAGCTCAGT GGTGTCCCGG AGAGACTCCC ATGCTTGGAC CCCACTGTAT 
GATTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

MicroRNA-141-3p targets DAPK1 and inhibits apoptosis in rat ovarian granulosa cells.
Cell biochemistry and function35(4)197-201(2017 Jun)
Li D,Xu D,Xu Y,Chen L,Li C,Dai X,Zhang L,Zheng L

Heterotrimeric GAIT complex drives transcript-selective translation inhibition in murine macrophages.
Molecular and cellular biology32(24)5046-55(2012 Dec)
Arif A,Chatterjee P,Moodt RA,Fox PL

PKD is a kinase of Vps34 that mediates ROS-induced autophagy downstream of DAPk.
Cell death and differentiation19(5)788-97(2012 May)
Eisenberg-Lerner A,Kimchi A

GTP binding to the ROC domain of DAP-kinase regulates its function through intramolecular signalling.
EMBO reports12(9)917-23(2011 Sep)
Carlessi R,Levin-Salomon V,Ciprut S,Bialik S,Berissi H,Albeck S,Peleg Y,Kimchi A

DAPK1 interaction with NMDA receptor NR2B subunits mediates brain damage in stroke.
Cell140(2)222-34(2010 Jan)
Tu W,Xu X,Peng L,Zhong X,Zhang W,Soundarapandian MM,Balel C,Wang M,Jia N,Zhang W,Lew F,Chan SL,Chen Y,Lu Y

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Dapk1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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