DAPK1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	DAPK1
Entrez Gene ID	540873
Full Name	death associated protein kinase 1
General protein information	Preferred Names death associated protein kinase 1 Names death-associated protein kinase 1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001206038.2	NP_001192967.1	death-associated protein kinase 1
NM_001206038.1	NP_001192967.1	death-associated protein kinase 1
XM_024995907.1	XP_024851675.1	death-associated protein kinase 1 isoform X1
XM_024995909.1	XP_024851677.1	death-associated protein kinase 1 isoform X1
XM_024995910.1	XP_024851678.1	death-associated protein kinase 1 isoform X1
XM_024995908.1	XP_024851676.1	death-associated protein kinase 1 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	DAPK1	NP_004929.2
Xenopus tropicalis (tropical clawed frog)	dapk1	NP_001116911.1
Rattus norvegicus (Norway rat)	Dapk1	NP_001100805.2
Danio rerio (zebrafish)	dapk1	NP_001093460.1
Pan troglodytes (chimpanzee)	DAPK1	XP_001140200.1
	DAPK1	XP_001084752.1
Bos taurus (cattle)	DAPK1	NP_001192967.1
Gallus gallus (chicken)	DAPK1	NP_001264960.1
Canis lupus familiaris (dog)	DAPK1	NP_001184086.1
Mus musculus (house mouse)	Dapk1	NP_598823.1
Caenorhabditis elegans (roundworm)	dapk-1	NP_490840.2

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following DAPK1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DAPK1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa237231	NM_001206038.2 Latest version!	Bos taurus death associated protein kinase 1 (DAPK1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa237231	XM_024995908.1 Latest version!	Bos taurus death associated protein kinase 1 (DAPK1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa237231	XM_024995910.1 Latest version!	Bos taurus death associated protein kinase 1 (DAPK1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa237231	XM_024995909.1 Latest version!	Bos taurus death associated protein kinase 1 (DAPK1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa237231	XM_024995907.1 Latest version!	Bos taurus death associated protein kinase 1 (DAPK1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa01719	NM_001206038.1 Latest version!	Bos taurus death-associated protein kinase 1 (DAPK1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DAPK1 ( NM_001206038.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001192967.1 Bos taurus death associated protein kinase 1 (DAPK1), mRNA.

Price & Availability↑

CloneID	OBa237231
Clone ID Related Accession (Same CDS sequence)	NM_001206038.2 , XM_024995907.1 , XM_024995909.1 , XM_024995910.1 , XM_024995908.1
Accession Version	NM_001206038.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4293bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-27
Organism	Bos taurus(cattle)
Product	death-associated protein kinase 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000008.1. On Jun 28, 2018 this sequence version replaced NM_001206038.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR6764281.40638.1, SRR5571258.4516.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145415 [ECO:0000348] ##Evidence-Data-END##

ATGACCGTGT TCAGGCAGGA AAACGTGGAT GATTACTACG ACACCGGCGA GGAACTTGGC 
AGTGGGCAGT TTGCCGTCGT GAAGAAATGC CGTGAGAAAA GCACCGGTCT ACAGTATGCC 
GCCAAATTCA TCAAGAAAAG GAGGACCAAA TCCAGCCGGC GGGGCGTGAG CCGCGAGGAC 
ATCGAGCGGG AGGTCAGCAT CCTGAAGGAG ATCCAGCACC CCAACGTCAT CACCCTGCAC 
GAGGTTTACG AGAACAAGAC GGACGTCATC CTCATCCTGG AACTCGTTGC AGGTGGTGAG 
CTGTTTGACT TCTTAGCTGA AAAGGAATCT TTGACTGAAG AGGAAGCAAC TGAATTTCTC 
AAACAAATTC TTAATGGTGT TTACTATCTG CACTCCCTTC AGATTGCCCA CTTTGATCTT 
AAGCCCGAAA ACATAATGCT TTTGGATAGA AATGTTCCCA AGCCTCGGAT CAAGATCATT 
GACTTTGGGT TGGCCCATAA AATTGACTTT GGAAATGAAT TCAAAAATAT ATTTGGGACA 
CCAGAATTTG TTGCTCCTGA GATAGTCAAC TATGAACCTC TTGGTCTTGA GGCAGATATG 
TGGAGTATTG GGGTAATAAC CTATATTCTC CTCAGTGGGG CCTCCCCGTT TCTCGGAGAC 
AGTAAGCAAG AAACGTTAGC GAACGTATCT GCCGTCAACT ATGAATTTGA GGAAGAGTAC 
TTCAGTAATA CGAGCGCCCT AGCCAAAGAT TTCATAAGAA GACTCCTCGT CAAGGATCCA 
AAGAAGAGAA TGACAATTCA AGATAGTTTG CAGCATCCCT GGATCAAGCC TAAAGATACC 
CAACAAGCAC TTAGTAGAAA AGCATCAGCA GTAAACATGG AGAAATTCAA GAAGTTTGCA 
GCCCGGAAAA AATGGAAACA ATCTGTTCGC TTGATATCAC TGTGCCAAAG ATTATCCAGG 
TCATTCTTGT CCAGAAGTAA CATGAGTGTT GCCAGAAGCG ATGATACTCT GGATGAGGAA 
GACTCCTTTG TGATGAAAGC TATCATCCAT GCCATCAACG ATGACAATGT CCCAGGCCTG 
CAGCACCTCC TGGGCTCCTT GTCCAACTAC GACGTTAACC AGCCCAACAA GCATGGGACT 
CCTCCATTAC TTATTGCTGC TGGCTGTGGG AATATTCAAA TACTACAGTT GCTCATTAAG 
AGAGGCTCAA GAATTGACAT CCAGGATAAG GGTGGATCCA ATGCCATCTA CTGGGCCTCT 
CGGCATGGCC ATGTAGATAC CTTGAAATTT CTCAATGAGA ACAAATGCCC TCTGGACGTT 
AAAGACAAGT CTGGAGAGAC AGCCCTTCAT GTGGCAGCTC GCTACGGCCA CGCTGATGTG 
GTTCAGTTAC TGTGCAGCTT CGGCTCGAAT CCTAACTTCC AGGACAAGGA AGAAGAAACC 
CCCCTGCACT GTGCTGCCTG GCACGGCTAT TACTCTGTGG CCAAAGCCCT TTGTGAAGCC 
GGCTGCAATG TGAACATTAA GAACAGAGAA GGCGAGACCC CCCTCCTGAC GGCCTCCGCC 
AGGGGCTACC AAGACATCGT GGAGTGTCTG GCCGAGCACG GAGCCGACCT TAATGCATCC 
GACAAGGATG GACACATTGC TCTACATCTT GCCGTGAGAC GATGTCAGAT GGAGGTCATC 
CAGACTCTCA TCAGCCAGGG CAGTTCCGTC GATTTCCAAG ACAGGCATGG CAACACCCCC 
CTCCATGTGG CCTGCAAAGA TGGCAACGTG CCGATTGTGC TGGCCCTCTG TGAAGCCAAC 
TGCAATTTGG ATCTCTCCAA CAAGTATGGA CGGACGCCTC TGCACCTTGC CGCCAACAAC 
GGGATTCTGG ACGTGGTCCG CTACCTGTGT CTGACGGGCG CCAATGTGGA GGCGCTGACC 
TCGGATGGAA AGACAGCAGA AGATCTTGCG AAATCGGAAC AGCATGAACA CGTAGCAGGC 
CTCCTCACAA GACTCCGAAA GGACACACAC CGAGGACTCT TCATCCAGCA ACTGCGACCC 
ACACAGAACC TGCAGCCAAG AATCAAGCTG AAGCTGTTCG GCCACTCGGG GTCTGGGAAG 
ACCACTCTGG TGGAATCTCT CAAGTGTGGG CTGCTAAGGA GCTTCTTCCG AAGACGCCGG 
CCCCGGCTCT CCTCCACCAA CTCTACCCGG TTCCCACCCT CGCCCCTGGC CTCCAAGCCG 
GCAGTGTCCG TGAGCATCAG CAACCTGTAC CCGGGCTGTG AGAACGTGAG TGTCAGGAGC 
CGCAGCATGA TGTTCGAGCC AGGCCTCACC AAGGGGGTGC TGGAGGTGTT CGGGGCCCCG 
TCCCACCATC CGCACTGCGC CACCGATGAC CAGTCCACGA AGGCAATCGA CATCCAGAAT 
GCCTATTTGA ACGGCATTGG TGACTTCAGT GTGTGGGAGT TCTCTGGAAA TCCTGTCTAC 
TTCTGCTGTT ATGACTATTT TGCTGCAAAC GATCCCACGT CCATCCATGT CATCGTTTTC 
AGTCTAGAAG AACCCTATGA GATTCAGCTG AATCAAGTGA TTTTCTGGCT TAGTTTCCTG 
AAGTCTCTTG TCCCAGTGGA AGAACCGATA GCCTTTGGCG GCAAGCTGAA GAACCCACTC 
CGAGTTGTCC TGGTGGCCAC CCATGCCGAC ATCGTGAACC TTCCTCGTTC AGCTGGAGGC 
GAGTTTGGAT ATGACAAAGA CACCTCGTTG CTGAAAGAGA TCAGGAACAG GTTTGGGAAC 
GATCTTCACA TTTCAAATAA GCTCTTTGTT CTGGATGCCG GGGCTTCTGG GTCTAAGGAC 
ATGAAGGTGC TTCGAAATCA CCTGCAAGAA ATACGGAGCC AGATTGTTTC GGTCTGCCCT 
CCAATGACTC ACCTATGTGA AAAGATCATC TCCACTTTGC CCTCCTGGAG GAAGCTCAAT 
GGCCCCAACC AGCTGATGTC ACTGCAGCAG TTCGTGTACG ACGTACAGGA CCAGTTGAAC 
CCCCTGGCCA GCGAGGCAGA CCTCCGGCAC ATCGCCCAGC AGCTCCACAG TGCTGGCGAG 
ATCAACATCA TGCAGAGCGA GACCGTCCAG GACGTGCTGC TCCTGGATCC CCGCTGGCTG 
TGCACCAACG TTCTGGGGAA GCTGCTCTCT GTGGAGACCC CACGGGCCCT GCACCATTAC 
CGCGGCCGCT ACACCATGGA GGACGTCCAG CGCCTGGTGC CCGACAGTGA CGTGGAGGAG 
CTGCTGCAGA TCCTGGATGC CATGGACATC TGTGCACGGG ACCTGAGCAG CGGGACCATG 
GTGGACATCC CCGCCCTGAT CAAGACTGAC AGCCTACACC GCTCCTGGAC GGAGGAGGAG 
GACGAGGCGC GGGTCTACGG CGGCGTGCGA GTCGTCCCGG TGGAGCACCT CACCCCCTTC 
CCATGTGGCA TCTTCCACAA GGTGCAGGTG AACCTGTGCC GGTGGATCCA CCAGCAGAGC 
AACGAGGGGG ATGCAGACAT TCGCCTGTGG GTGAACGGCT GCAAGATCGC CAATCGCGGG 
GCCGAGCTGC TGGTGCTCCT GGTCAACCAC GGCCAGGGTA TTGAGGTCCA GGTGCGTGGA 
CTGGAGACGG AGAAGATCAA GTGCTGCTTC CTGCTGGACT CCGTGTGCAG CACCATCGAG 
AACGTCATGG CCACCACGCT GCCCGGGCTG CTGACGGTGA AGCACTACCT GAGCCCCCAG 
CAGCTACGAG AGCACCACGA GCCCGTCATG ATCTACCAGC CACGGGACTT CTTCCGGGCG 
CAGACTCTCA AGGAGACCTC ACTGACCAAC ACCATGGGCG GGTACAAGGA GAGCTTTAGC 
AGTATCATGT GCTTCGGCTG TCACGACGTC TACTCGCAGG CCAGCCTCGG CATGGACATC 
CACGCGTCAG ATCTGAACCT CCTGACCCGG AGGAAACTCA GTCGCCTGCT GGACCCGCCC 
GACCCCATGG GGAAGGACTG GTGCCTCCTT GCCATGAACT TGGGCCTCCC CGACCTTGTG 
GCCAAGTACA ACACCAATAA CGGGTCTCCC AAGGAGTTCC TCCCGAGCCC AGTCCACGCC 
CTGCTCCGGG AATGGACTTC CTACCCCGAG AGCACTGTCG GCATTCTCAT GTCCAAACTG 
CGGGAGCTGG GGCGTCGGGA CGCAGCGGAC TTCCTGCTGA AGGCGTCCTC TGTGTTCAAA 
ATCAACCTCG ACGGCAACGG CCAGGAGGCC TACGCCTCAA GCTGCAACAG TGGCACATCT 
TACAATTCCA TTAGCTCGGT CGTGTCCCGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001192967.1
CDS	296..4588
Translation	MTVFRQENVDDYYDTGEELGSGQFAVVKKCREKSTGLQYAAKFIKKRRTKSSRRGVSREDIEREVSILKEIQHPNVITLHEVYENKTDVILILELVAGGELFDFLAEKESLTEEEATEFLKQILNGVYYLHSLQIAHFDLKPENIMLLDRNVPKPRIKIIDFGLAHKIDFGNEFKNIFGTPEFVAPEIVNYEPLGLEADMWSIGVITYILLSGASPFLGDSKQETLANVSAVNYEFEEEYFSNTSALAKDFIRRLLVKDPKKRMTIQDSLQHPWIKPKDTQQALSRKASAVNMEKFKKFAARKKWKQSVRLISLCQRLSRSFLSRSNMSVARSDDTLDEEDSFVMKAIIHAINDDNVPGLQHLLGSLSNYDVNQPNKHGTPPLLIAAGCGNIQILQLLIKRGSRIDIQDKGGSNAIYWASRHGHVDTLKFLNENKCPLDVKDKSGETALHVAARYGHADVVQLLCSFGSNPNFQDKEEETPLHCAAWHGYYSVAKALCEAGCNVNIKNREGETPLLTASARGYQDIVECLAEHGADLNASDKDGHIALHLAVRRCQMEVIQTLISQGSSVDFQDRHGNTPLHVACKDGNVPIVLALCEANCNLDLSNKYGRTPLHLAANNGILDVVRYLCLTGANVEALTSDGKTAEDLAKSEQHEHVAGLLTRLRKDTHRGLFIQQLRPTQNLQPRIKLKLFGHSGSGKTTLVESLKCGLLRSFFRRRRPRLSSTNSTRFPPSPLASKPAVSVSISNLYPGCENVSVRSRSMMFEPGLTKGVLEVFGAPSHHPHCATDDQSTKAIDIQNAYLNGIGDFSVWEFSGNPVYFCCYDYFAANDPTSIHVIVFSLEEPYEIQLNQVIFWLSFLKSLVPVEEPIAFGGKLKNPLRVVLVATHADIVNLPRSAGGEFGYDKDTSLLKEIRNRFGNDLHISNKLFVLDAGASGSKDMKVLRNHLQEIRSQIVSVCPPMTHLCEKIISTLPSWRKLNGPNQLMSLQQFVYDVQDQLNPLASEADLRHIAQQLHSAGEINIMQSETVQDVLLLDPRWLCTNVLGKLLSVETPRALHHYRGRYTMEDVQRLVPDSDVEELLQILDAMDICARDLSSGTMVDIPALIKTDSLHRSWTEEEDEARVYGGVRVVPVEHLTPFPCGIFHKVQVNLCRWIHQQSNEGDADIRLWVNGCKIANRGAELLVLLVNHGQGIEVQVRGLETEKIKCCFLLDSVCSTIENVMATTLPGLLTVKHYLSPQQLREHHEPVMIYQPRDFFRAQTLKETSLTNTMGGYKESFSSIMCFGCHDVYSQASLGMDIHASDLNLLTRRKLSRLLDPPDPMGKDWCLLAMNLGLPDLVAKYNTNNGSPKEFLPSPVHALLREWTSYPESTVGILMSKLRELGRRDAADFLLKASSVFKINLDGNGQEAYASSCNSGTSYNSISSVVSR

Target ORF information:

RefSeq Version	NM_001206038.2
Organism	Bos taurus(cattle)
Definition	Bos taurus death associated protein kinase 1 (DAPK1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001206038.2

ORF Insert Sequence:

ATGACCGTGT TCAGGCAGGA AAACGTGGAT GATTACTACG ACACCGGCGA GGAACTTGGC 
AGTGGGCAGT TTGCCGTCGT GAAGAAATGC CGTGAGAAAA GCACCGGTCT ACAGTATGCC 
GCCAAATTCA TCAAGAAAAG GAGGACCAAA TCCAGCCGGC GGGGCGTGAG CCGCGAGGAC 
ATCGAGCGGG AGGTCAGCAT CCTGAAGGAG ATCCAGCACC CCAACGTCAT CACCCTGCAC 
GAGGTTTACG AGAACAAGAC GGACGTCATC CTCATCCTGG AACTCGTTGC AGGTGGTGAG 
CTGTTTGACT TCTTAGCTGA AAAGGAATCT TTGACTGAAG AGGAAGCAAC TGAATTTCTC 
AAACAAATTC TTAATGGTGT TTACTATCTG CACTCCCTTC AGATTGCCCA CTTTGATCTT 
AAGCCCGAAA ACATAATGCT TTTGGATAGA AATGTTCCCA AGCCTCGGAT CAAGATCATT 
GACTTTGGGT TGGCCCATAA AATTGACTTT GGAAATGAAT TCAAAAATAT ATTTGGGACA 
CCAGAATTTG TTGCTCCTGA GATAGTCAAC TATGAACCTC TTGGTCTTGA GGCAGATATG 
TGGAGTATTG GGGTAATAAC CTATATTCTC CTCAGTGGGG CCTCCCCGTT TCTCGGAGAC 
AGTAAGCAAG AAACGTTAGC GAACGTATCT GCCGTCAACT ATGAATTTGA GGAAGAGTAC 
TTCAGTAATA CGAGCGCCCT AGCCAAAGAT TTCATAAGAA GACTCCTCGT CAAGGATCCA 
AAGAAGAGAA TGACAATTCA AGATAGTTTG CAGCATCCCT GGATCAAGCC TAAAGATACC 
CAACAAGCAC TTAGTAGAAA AGCATCAGCA GTAAACATGG AGAAATTCAA GAAGTTTGCA 
GCCCGGAAAA AATGGAAACA ATCTGTTCGC TTGATATCAC TGTGCCAAAG ATTATCCAGG 
TCATTCTTGT CCAGAAGTAA CATGAGTGTT GCCAGAAGCG ATGATACTCT GGATGAGGAA 
GACTCCTTTG TGATGAAAGC TATCATCCAT GCCATCAACG ATGACAATGT CCCAGGCCTG 
CAGCACCTCC TGGGCTCCTT GTCCAACTAC GACGTTAACC AGCCCAACAA GCATGGGACT 
CCTCCATTAC TTATTGCTGC TGGCTGTGGG AATATTCAAA TACTACAGTT GCTCATTAAG 
AGAGGCTCAA GAATTGACAT CCAGGATAAG GGTGGATCCA ATGCCATCTA CTGGGCCTCT 
CGGCATGGCC ATGTAGATAC CTTGAAATTT CTCAATGAGA ACAAATGCCC TCTGGACGTT 
AAAGACAAGT CTGGAGAGAC AGCCCTTCAT GTGGCAGCTC GCTACGGCCA CGCTGATGTG 
GTTCAGTTAC TGTGCAGCTT CGGCTCGAAT CCTAACTTCC AGGACAAGGA AGAAGAAACC 
CCCCTGCACT GTGCTGCCTG GCACGGCTAT TACTCTGTGG CCAAAGCCCT TTGTGAAGCC 
GGCTGCAATG TGAACATTAA GAACAGAGAA GGCGAGACCC CCCTCCTGAC GGCCTCCGCC 
AGGGGCTACC AAGACATCGT GGAGTGTCTG GCCGAGCACG GAGCCGACCT TAATGCATCC 
GACAAGGATG GACACATTGC TCTACATCTT GCCGTGAGAC GATGTCAGAT GGAGGTCATC 
CAGACTCTCA TCAGCCAGGG CAGTTCCGTC GATTTCCAAG ACAGGCATGG CAACACCCCC 
CTCCATGTGG CCTGCAAAGA TGGCAACGTG CCGATTGTGC TGGCCCTCTG TGAAGCCAAC 
TGCAATTTGG ATCTCTCCAA CAAGTATGGA CGGACGCCTC TGCACCTTGC CGCCAACAAC 
GGGATTCTGG ACGTGGTCCG CTACCTGTGT CTGACGGGCG CCAATGTGGA GGCGCTGACC 
TCGGATGGAA AGACAGCAGA AGATCTTGCG AAATCGGAAC AGCATGAACA CGTAGCAGGC 
CTCCTCACAA GACTCCGAAA GGACACACAC CGAGGACTCT TCATCCAGCA ACTGCGACCC 
ACACAGAACC TGCAGCCAAG AATCAAGCTG AAGCTGTTCG GCCACTCGGG GTCTGGGAAG 
ACCACTCTGG TGGAATCTCT CAAGTGTGGG CTGCTAAGGA GCTTCTTCCG AAGACGCCGG 
CCCCGGCTCT CCTCCACCAA CTCTACCCGG TTCCCACCCT CGCCCCTGGC CTCCAAGCCG 
GCAGTGTCCG TGAGCATCAG CAACCTGTAC CCGGGCTGTG AGAACGTGAG TGTCAGGAGC 
CGCAGCATGA TGTTCGAGCC AGGCCTCACC AAGGGGGTGC TGGAGGTGTT CGGGGCCCCG 
TCCCACCATC CGCACTGCGC CACCGATGAC CAGTCCACGA AGGCAATCGA CATCCAGAAT 
GCCTATTTGA ACGGCATTGG TGACTTCAGT GTGTGGGAGT TCTCTGGAAA TCCTGTCTAC 
TTCTGCTGTT ATGACTATTT TGCTGCAAAC GATCCCACGT CCATCCATGT CATCGTTTTC 
AGTCTAGAAG AACCCTATGA GATTCAGCTG AATCAAGTGA TTTTCTGGCT TAGTTTCCTG 
AAGTCTCTTG TCCCAGTGGA AGAACCGATA GCCTTTGGCG GCAAGCTGAA GAACCCACTC 
CGAGTTGTCC TGGTGGCCAC CCATGCCGAC ATCGTGAACC TTCCTCGTTC AGCTGGAGGC 
GAGTTTGGAT ATGACAAAGA CACCTCGTTG CTGAAAGAGA TCAGGAACAG GTTTGGGAAC 
GATCTTCACA TTTCAAATAA GCTCTTTGTT CTGGATGCCG GGGCTTCTGG GTCTAAGGAC 
ATGAAGGTGC TTCGAAATCA CCTGCAAGAA ATACGGAGCC AGATTGTTTC GGTCTGCCCT 
CCAATGACTC ACCTATGTGA AAAGATCATC TCCACTTTGC CCTCCTGGAG GAAGCTCAAT 
GGCCCCAACC AGCTGATGTC ACTGCAGCAG TTCGTGTACG ACGTACAGGA CCAGTTGAAC 
CCCCTGGCCA GCGAGGCAGA CCTCCGGCAC ATCGCCCAGC AGCTCCACAG TGCTGGCGAG 
ATCAACATCA TGCAGAGCGA GACCGTCCAG GACGTGCTGC TCCTGGATCC CCGCTGGCTG 
TGCACCAACG TTCTGGGGAA GCTGCTCTCT GTGGAGACCC CACGGGCCCT GCACCATTAC 
CGCGGCCGCT ACACCATGGA GGACGTCCAG CGCCTGGTGC CCGACAGTGA CGTGGAGGAG 
CTGCTGCAGA TCCTGGATGC CATGGACATC TGTGCACGGG ACCTGAGCAG CGGGACCATG 
GTGGACATCC CCGCCCTGAT CAAGACTGAC AGCCTACACC GCTCCTGGAC GGAGGAGGAG 
GACGAGGCGC GGGTCTACGG CGGCGTGCGA GTCGTCCCGG TGGAGCACCT CACCCCCTTC 
CCATGTGGCA TCTTCCACAA GGTGCAGGTG AACCTGTGCC GGTGGATCCA CCAGCAGAGC 
AACGAGGGGG ATGCAGACAT TCGCCTGTGG GTGAACGGCT GCAAGATCGC CAATCGCGGG 
GCCGAGCTGC TGGTGCTCCT GGTCAACCAC GGCCAGGGTA TTGAGGTCCA GGTGCGTGGA 
CTGGAGACGG AGAAGATCAA GTGCTGCTTC CTGCTGGACT CCGTGTGCAG CACCATCGAG 
AACGTCATGG CCACCACGCT GCCCGGGCTG CTGACGGTGA AGCACTACCT GAGCCCCCAG 
CAGCTACGAG AGCACCACGA GCCCGTCATG ATCTACCAGC CACGGGACTT CTTCCGGGCG 
CAGACTCTCA AGGAGACCTC ACTGACCAAC ACCATGGGCG GGTACAAGGA GAGCTTTAGC 
AGTATCATGT GCTTCGGCTG TCACGACGTC TACTCGCAGG CCAGCCTCGG CATGGACATC 
CACGCGTCAG ATCTGAACCT CCTGACCCGG AGGAAACTCA GTCGCCTGCT GGACCCGCCC 
GACCCCATGG GGAAGGACTG GTGCCTCCTT GCCATGAACT TGGGCCTCCC CGACCTTGTG 
GCCAAGTACA ACACCAATAA CGGGTCTCCC AAGGAGTTCC TCCCGAGCCC AGTCCACGCC 
CTGCTCCGGG AATGGACTTC CTACCCCGAG AGCACTGTCG GCATTCTCAT GTCCAAACTG 
CGGGAGCTGG GGCGTCGGGA CGCAGCGGAC TTCCTGCTGA AGGCGTCCTC TGTGTTCAAA 
ATCAACCTCG ACGGCAACGG CCAGGAGGCC TACGCCTCAA GCTGCAACAG TGGCACATCT 
TACAATTCCA TTAGCTCGGT CGTGTCCCGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DAPK1 ( XM_024995907.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024851675.1 Bos taurus death associated protein kinase 1 (DAPK1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa237231
Clone ID Related Accession (Same CDS sequence)	NM_001206038.2 , XM_024995907.1 , XM_024995909.1 , XM_024995910.1 , XM_024995908.1
Accession Version	XM_024995907.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4293bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	death-associated protein kinase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGTGT TCAGGCAGGA AAACGTGGAT GATTACTACG ACACCGGCGA GGAACTTGGC 
AGTGGGCAGT TTGCCGTCGT GAAGAAATGC CGTGAGAAAA GCACCGGTCT ACAGTATGCC 
GCCAAATTCA TCAAGAAAAG GAGGACCAAA TCCAGCCGGC GGGGCGTGAG CCGCGAGGAC 
ATCGAGCGGG AGGTCAGCAT CCTGAAGGAG ATCCAGCACC CCAACGTCAT CACCCTGCAC 
GAGGTTTACG AGAACAAGAC GGACGTCATC CTCATCCTGG AACTCGTTGC AGGTGGTGAG 
CTGTTTGACT TCTTAGCTGA AAAGGAATCT TTGACTGAAG AGGAAGCAAC TGAATTTCTC 
AAACAAATTC TTAATGGTGT TTACTATCTG CACTCCCTTC AGATTGCCCA CTTTGATCTT 
AAGCCCGAAA ACATAATGCT TTTGGATAGA AATGTTCCCA AGCCTCGGAT CAAGATCATT 
GACTTTGGGT TGGCCCATAA AATTGACTTT GGAAATGAAT TCAAAAATAT ATTTGGGACA 
CCAGAATTTG TTGCTCCTGA GATAGTCAAC TATGAACCTC TTGGTCTTGA GGCAGATATG 
TGGAGTATTG GGGTAATAAC CTATATTCTC CTCAGTGGGG CCTCCCCGTT TCTCGGAGAC 
AGTAAGCAAG AAACGTTAGC GAACGTATCT GCCGTCAACT ATGAATTTGA GGAAGAGTAC 
TTCAGTAATA CGAGCGCCCT AGCCAAAGAT TTCATAAGAA GACTCCTCGT CAAGGATCCA 
AAGAAGAGAA TGACAATTCA AGATAGTTTG CAGCATCCCT GGATCAAGCC TAAAGATACC 
CAACAAGCAC TTAGTAGAAA AGCATCAGCA GTAAACATGG AGAAATTCAA GAAGTTTGCA 
GCCCGGAAAA AATGGAAACA ATCTGTTCGC TTGATATCAC TGTGCCAAAG ATTATCCAGG 
TCATTCTTGT CCAGAAGTAA CATGAGTGTT GCCAGAAGCG ATGATACTCT GGATGAGGAA 
GACTCCTTTG TGATGAAAGC TATCATCCAT GCCATCAACG ATGACAATGT CCCAGGCCTG 
CAGCACCTCC TGGGCTCCTT GTCCAACTAC GACGTTAACC AGCCCAACAA GCATGGGACT 
CCTCCATTAC TTATTGCTGC TGGCTGTGGG AATATTCAAA TACTACAGTT GCTCATTAAG 
AGAGGCTCAA GAATTGACAT CCAGGATAAG GGTGGATCCA ATGCCATCTA CTGGGCCTCT 
CGGCATGGCC ATGTAGATAC CTTGAAATTT CTCAATGAGA ACAAATGCCC TCTGGACGTT 
AAAGACAAGT CTGGAGAGAC AGCCCTTCAT GTGGCAGCTC GCTACGGCCA CGCTGATGTG 
GTTCAGTTAC TGTGCAGCTT CGGCTCGAAT CCTAACTTCC AGGACAAGGA AGAAGAAACC 
CCCCTGCACT GTGCTGCCTG GCACGGCTAT TACTCTGTGG CCAAAGCCCT TTGTGAAGCC 
GGCTGCAATG TGAACATTAA GAACAGAGAA GGCGAGACCC CCCTCCTGAC GGCCTCCGCC 
AGGGGCTACC AAGACATCGT GGAGTGTCTG GCCGAGCACG GAGCCGACCT TAATGCATCC 
GACAAGGATG GACACATTGC TCTACATCTT GCCGTGAGAC GATGTCAGAT GGAGGTCATC 
CAGACTCTCA TCAGCCAGGG CAGTTCCGTC GATTTCCAAG ACAGGCATGG CAACACCCCC 
CTCCATGTGG CCTGCAAAGA TGGCAACGTG CCGATTGTGC TGGCCCTCTG TGAAGCCAAC 
TGCAATTTGG ATCTCTCCAA CAAGTATGGA CGGACGCCTC TGCACCTTGC CGCCAACAAC 
GGGATTCTGG ACGTGGTCCG CTACCTGTGT CTGACGGGCG CCAATGTGGA GGCGCTGACC 
TCGGATGGAA AGACAGCAGA AGATCTTGCG AAATCGGAAC AGCATGAACA CGTAGCAGGC 
CTCCTCACAA GACTCCGAAA GGACACACAC CGAGGACTCT TCATCCAGCA ACTGCGACCC 
ACACAGAACC TGCAGCCAAG AATCAAGCTG AAGCTGTTCG GCCACTCGGG GTCTGGGAAG 
ACCACTCTGG TGGAATCTCT CAAGTGTGGG CTGCTAAGGA GCTTCTTCCG AAGACGCCGG 
CCCCGGCTCT CCTCCACCAA CTCTACCCGG TTCCCACCCT CGCCCCTGGC CTCCAAGCCG 
GCAGTGTCCG TGAGCATCAG CAACCTGTAC CCGGGCTGTG AGAACGTGAG TGTCAGGAGC 
CGCAGCATGA TGTTCGAGCC AGGCCTCACC AAGGGGGTGC TGGAGGTGTT CGGGGCCCCG 
TCCCACCATC CGCACTGCGC CACCGATGAC CAGTCCACGA AGGCAATCGA CATCCAGAAT 
GCCTATTTGA ACGGCATTGG TGACTTCAGT GTGTGGGAGT TCTCTGGAAA TCCTGTCTAC 
TTCTGCTGTT ATGACTATTT TGCTGCAAAC GATCCCACGT CCATCCATGT CATCGTTTTC 
AGTCTAGAAG AACCCTATGA GATTCAGCTG AATCAAGTGA TTTTCTGGCT TAGTTTCCTG 
AAGTCTCTTG TCCCAGTGGA AGAACCGATA GCCTTTGGCG GCAAGCTGAA GAACCCACTC 
CGAGTTGTCC TGGTGGCCAC CCATGCCGAC ATCGTGAACC TTCCTCGTTC AGCTGGAGGC 
GAGTTTGGAT ATGACAAAGA CACCTCGTTG CTGAAAGAGA TCAGGAACAG GTTTGGGAAC 
GATCTTCACA TTTCAAATAA GCTCTTTGTT CTGGATGCCG GGGCTTCTGG GTCTAAGGAC 
ATGAAGGTGC TTCGAAATCA CCTGCAAGAA ATACGGAGCC AGATTGTTTC GGTCTGCCCT 
CCAATGACTC ACCTATGTGA AAAGATCATC TCCACTTTGC CCTCCTGGAG GAAGCTCAAT 
GGCCCCAACC AGCTGATGTC ACTGCAGCAG TTCGTGTACG ACGTACAGGA CCAGTTGAAC 
CCCCTGGCCA GCGAGGCAGA CCTCCGGCAC ATCGCCCAGC AGCTCCACAG TGCTGGCGAG 
ATCAACATCA TGCAGAGCGA GACCGTCCAG GACGTGCTGC TCCTGGATCC CCGCTGGCTG 
TGCACCAACG TTCTGGGGAA GCTGCTCTCT GTGGAGACCC CACGGGCCCT GCACCATTAC 
CGCGGCCGCT ACACCATGGA GGACGTCCAG CGCCTGGTGC CCGACAGTGA CGTGGAGGAG 
CTGCTGCAGA TCCTGGATGC CATGGACATC TGTGCACGGG ACCTGAGCAG CGGGACCATG 
GTGGACATCC CCGCCCTGAT CAAGACTGAC AGCCTACACC GCTCCTGGAC GGAGGAGGAG 
GACGAGGCGC GGGTCTACGG CGGCGTGCGA GTCGTCCCGG TGGAGCACCT CACCCCCTTC 
CCATGTGGCA TCTTCCACAA GGTGCAGGTG AACCTGTGCC GGTGGATCCA CCAGCAGAGC 
AACGAGGGGG ATGCAGACAT TCGCCTGTGG GTGAACGGCT GCAAGATCGC CAATCGCGGG 
GCCGAGCTGC TGGTGCTCCT GGTCAACCAC GGCCAGGGTA TTGAGGTCCA GGTGCGTGGA 
CTGGAGACGG AGAAGATCAA GTGCTGCTTC CTGCTGGACT CCGTGTGCAG CACCATCGAG 
AACGTCATGG CCACCACGCT GCCCGGGCTG CTGACGGTGA AGCACTACCT GAGCCCCCAG 
CAGCTACGAG AGCACCACGA GCCCGTCATG ATCTACCAGC CACGGGACTT CTTCCGGGCG 
CAGACTCTCA AGGAGACCTC ACTGACCAAC ACCATGGGCG GGTACAAGGA GAGCTTTAGC 
AGTATCATGT GCTTCGGCTG TCACGACGTC TACTCGCAGG CCAGCCTCGG CATGGACATC 
CACGCGTCAG ATCTGAACCT CCTGACCCGG AGGAAACTCA GTCGCCTGCT GGACCCGCCC 
GACCCCATGG GGAAGGACTG GTGCCTCCTT GCCATGAACT TGGGCCTCCC CGACCTTGTG 
GCCAAGTACA ACACCAATAA CGGGTCTCCC AAGGAGTTCC TCCCGAGCCC AGTCCACGCC 
CTGCTCCGGG AATGGACTTC CTACCCCGAG AGCACTGTCG GCATTCTCAT GTCCAAACTG 
CGGGAGCTGG GGCGTCGGGA CGCAGCGGAC TTCCTGCTGA AGGCGTCCTC TGTGTTCAAA 
ATCAACCTCG ACGGCAACGG CCAGGAGGCC TACGCCTCAA GCTGCAACAG TGGCACATCT 
TACAATTCCA TTAGCTCGGT CGTGTCCCGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024851675.1
CDS	228..4520
Translation	MTVFRQENVDDYYDTGEELGSGQFAVVKKCREKSTGLQYAAKFIKKRRTKSSRRGVSREDIEREVSILKEIQHPNVITLHEVYENKTDVILILELVAGGELFDFLAEKESLTEEEATEFLKQILNGVYYLHSLQIAHFDLKPENIMLLDRNVPKPRIKIIDFGLAHKIDFGNEFKNIFGTPEFVAPEIVNYEPLGLEADMWSIGVITYILLSGASPFLGDSKQETLANVSAVNYEFEEEYFSNTSALAKDFIRRLLVKDPKKRMTIQDSLQHPWIKPKDTQQALSRKASAVNMEKFKKFAARKKWKQSVRLISLCQRLSRSFLSRSNMSVARSDDTLDEEDSFVMKAIIHAINDDNVPGLQHLLGSLSNYDVNQPNKHGTPPLLIAAGCGNIQILQLLIKRGSRIDIQDKGGSNAIYWASRHGHVDTLKFLNENKCPLDVKDKSGETALHVAARYGHADVVQLLCSFGSNPNFQDKEEETPLHCAAWHGYYSVAKALCEAGCNVNIKNREGETPLLTASARGYQDIVECLAEHGADLNASDKDGHIALHLAVRRCQMEVIQTLISQGSSVDFQDRHGNTPLHVACKDGNVPIVLALCEANCNLDLSNKYGRTPLHLAANNGILDVVRYLCLTGANVEALTSDGKTAEDLAKSEQHEHVAGLLTRLRKDTHRGLFIQQLRPTQNLQPRIKLKLFGHSGSGKTTLVESLKCGLLRSFFRRRRPRLSSTNSTRFPPSPLASKPAVSVSISNLYPGCENVSVRSRSMMFEPGLTKGVLEVFGAPSHHPHCATDDQSTKAIDIQNAYLNGIGDFSVWEFSGNPVYFCCYDYFAANDPTSIHVIVFSLEEPYEIQLNQVIFWLSFLKSLVPVEEPIAFGGKLKNPLRVVLVATHADIVNLPRSAGGEFGYDKDTSLLKEIRNRFGNDLHISNKLFVLDAGASGSKDMKVLRNHLQEIRSQIVSVCPPMTHLCEKIISTLPSWRKLNGPNQLMSLQQFVYDVQDQLNPLASEADLRHIAQQLHSAGEINIMQSETVQDVLLLDPRWLCTNVLGKLLSVETPRALHHYRGRYTMEDVQRLVPDSDVEELLQILDAMDICARDLSSGTMVDIPALIKTDSLHRSWTEEEDEARVYGGVRVVPVEHLTPFPCGIFHKVQVNLCRWIHQQSNEGDADIRLWVNGCKIANRGAELLVLLVNHGQGIEVQVRGLETEKIKCCFLLDSVCSTIENVMATTLPGLLTVKHYLSPQQLREHHEPVMIYQPRDFFRAQTLKETSLTNTMGGYKESFSSIMCFGCHDVYSQASLGMDIHASDLNLLTRRKLSRLLDPPDPMGKDWCLLAMNLGLPDLVAKYNTNNGSPKEFLPSPVHALLREWTSYPESTVGILMSKLRELGRRDAADFLLKASSVFKINLDGNGQEAYASSCNSGTSYNSISSVVSR

Target ORF information:

RefSeq Version	XM_024995907.1
Organism	Bos taurus(cattle)
Definition	Bos taurus death associated protein kinase 1 (DAPK1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024995907.1

ORF Insert Sequence:

ATGACCGTGT TCAGGCAGGA AAACGTGGAT GATTACTACG ACACCGGCGA GGAACTTGGC 
AGTGGGCAGT TTGCCGTCGT GAAGAAATGC CGTGAGAAAA GCACCGGTCT ACAGTATGCC 
GCCAAATTCA TCAAGAAAAG GAGGACCAAA TCCAGCCGGC GGGGCGTGAG CCGCGAGGAC 
ATCGAGCGGG AGGTCAGCAT CCTGAAGGAG ATCCAGCACC CCAACGTCAT CACCCTGCAC 
GAGGTTTACG AGAACAAGAC GGACGTCATC CTCATCCTGG AACTCGTTGC AGGTGGTGAG 
CTGTTTGACT TCTTAGCTGA AAAGGAATCT TTGACTGAAG AGGAAGCAAC TGAATTTCTC 
AAACAAATTC TTAATGGTGT TTACTATCTG CACTCCCTTC AGATTGCCCA CTTTGATCTT 
AAGCCCGAAA ACATAATGCT TTTGGATAGA AATGTTCCCA AGCCTCGGAT CAAGATCATT 
GACTTTGGGT TGGCCCATAA AATTGACTTT GGAAATGAAT TCAAAAATAT ATTTGGGACA 
CCAGAATTTG TTGCTCCTGA GATAGTCAAC TATGAACCTC TTGGTCTTGA GGCAGATATG 
TGGAGTATTG GGGTAATAAC CTATATTCTC CTCAGTGGGG CCTCCCCGTT TCTCGGAGAC 
AGTAAGCAAG AAACGTTAGC GAACGTATCT GCCGTCAACT ATGAATTTGA GGAAGAGTAC 
TTCAGTAATA CGAGCGCCCT AGCCAAAGAT TTCATAAGAA GACTCCTCGT CAAGGATCCA 
AAGAAGAGAA TGACAATTCA AGATAGTTTG CAGCATCCCT GGATCAAGCC TAAAGATACC 
CAACAAGCAC TTAGTAGAAA AGCATCAGCA GTAAACATGG AGAAATTCAA GAAGTTTGCA 
GCCCGGAAAA AATGGAAACA ATCTGTTCGC TTGATATCAC TGTGCCAAAG ATTATCCAGG 
TCATTCTTGT CCAGAAGTAA CATGAGTGTT GCCAGAAGCG ATGATACTCT GGATGAGGAA 
GACTCCTTTG TGATGAAAGC TATCATCCAT GCCATCAACG ATGACAATGT CCCAGGCCTG 
CAGCACCTCC TGGGCTCCTT GTCCAACTAC GACGTTAACC AGCCCAACAA GCATGGGACT 
CCTCCATTAC TTATTGCTGC TGGCTGTGGG AATATTCAAA TACTACAGTT GCTCATTAAG 
AGAGGCTCAA GAATTGACAT CCAGGATAAG GGTGGATCCA ATGCCATCTA CTGGGCCTCT 
CGGCATGGCC ATGTAGATAC CTTGAAATTT CTCAATGAGA ACAAATGCCC TCTGGACGTT 
AAAGACAAGT CTGGAGAGAC AGCCCTTCAT GTGGCAGCTC GCTACGGCCA CGCTGATGTG 
GTTCAGTTAC TGTGCAGCTT CGGCTCGAAT CCTAACTTCC AGGACAAGGA AGAAGAAACC 
CCCCTGCACT GTGCTGCCTG GCACGGCTAT TACTCTGTGG CCAAAGCCCT TTGTGAAGCC 
GGCTGCAATG TGAACATTAA GAACAGAGAA GGCGAGACCC CCCTCCTGAC GGCCTCCGCC 
AGGGGCTACC AAGACATCGT GGAGTGTCTG GCCGAGCACG GAGCCGACCT TAATGCATCC 
GACAAGGATG GACACATTGC TCTACATCTT GCCGTGAGAC GATGTCAGAT GGAGGTCATC 
CAGACTCTCA TCAGCCAGGG CAGTTCCGTC GATTTCCAAG ACAGGCATGG CAACACCCCC 
CTCCATGTGG CCTGCAAAGA TGGCAACGTG CCGATTGTGC TGGCCCTCTG TGAAGCCAAC 
TGCAATTTGG ATCTCTCCAA CAAGTATGGA CGGACGCCTC TGCACCTTGC CGCCAACAAC 
GGGATTCTGG ACGTGGTCCG CTACCTGTGT CTGACGGGCG CCAATGTGGA GGCGCTGACC 
TCGGATGGAA AGACAGCAGA AGATCTTGCG AAATCGGAAC AGCATGAACA CGTAGCAGGC 
CTCCTCACAA GACTCCGAAA GGACACACAC CGAGGACTCT TCATCCAGCA ACTGCGACCC 
ACACAGAACC TGCAGCCAAG AATCAAGCTG AAGCTGTTCG GCCACTCGGG GTCTGGGAAG 
ACCACTCTGG TGGAATCTCT CAAGTGTGGG CTGCTAAGGA GCTTCTTCCG AAGACGCCGG 
CCCCGGCTCT CCTCCACCAA CTCTACCCGG TTCCCACCCT CGCCCCTGGC CTCCAAGCCG 
GCAGTGTCCG TGAGCATCAG CAACCTGTAC CCGGGCTGTG AGAACGTGAG TGTCAGGAGC 
CGCAGCATGA TGTTCGAGCC AGGCCTCACC AAGGGGGTGC TGGAGGTGTT CGGGGCCCCG 
TCCCACCATC CGCACTGCGC CACCGATGAC CAGTCCACGA AGGCAATCGA CATCCAGAAT 
GCCTATTTGA ACGGCATTGG TGACTTCAGT GTGTGGGAGT TCTCTGGAAA TCCTGTCTAC 
TTCTGCTGTT ATGACTATTT TGCTGCAAAC GATCCCACGT CCATCCATGT CATCGTTTTC 
AGTCTAGAAG AACCCTATGA GATTCAGCTG AATCAAGTGA TTTTCTGGCT TAGTTTCCTG 
AAGTCTCTTG TCCCAGTGGA AGAACCGATA GCCTTTGGCG GCAAGCTGAA GAACCCACTC 
CGAGTTGTCC TGGTGGCCAC CCATGCCGAC ATCGTGAACC TTCCTCGTTC AGCTGGAGGC 
GAGTTTGGAT ATGACAAAGA CACCTCGTTG CTGAAAGAGA TCAGGAACAG GTTTGGGAAC 
GATCTTCACA TTTCAAATAA GCTCTTTGTT CTGGATGCCG GGGCTTCTGG GTCTAAGGAC 
ATGAAGGTGC TTCGAAATCA CCTGCAAGAA ATACGGAGCC AGATTGTTTC GGTCTGCCCT 
CCAATGACTC ACCTATGTGA AAAGATCATC TCCACTTTGC CCTCCTGGAG GAAGCTCAAT 
GGCCCCAACC AGCTGATGTC ACTGCAGCAG TTCGTGTACG ACGTACAGGA CCAGTTGAAC 
CCCCTGGCCA GCGAGGCAGA CCTCCGGCAC ATCGCCCAGC AGCTCCACAG TGCTGGCGAG 
ATCAACATCA TGCAGAGCGA GACCGTCCAG GACGTGCTGC TCCTGGATCC CCGCTGGCTG 
TGCACCAACG TTCTGGGGAA GCTGCTCTCT GTGGAGACCC CACGGGCCCT GCACCATTAC 
CGCGGCCGCT ACACCATGGA GGACGTCCAG CGCCTGGTGC CCGACAGTGA CGTGGAGGAG 
CTGCTGCAGA TCCTGGATGC CATGGACATC TGTGCACGGG ACCTGAGCAG CGGGACCATG 
GTGGACATCC CCGCCCTGAT CAAGACTGAC AGCCTACACC GCTCCTGGAC GGAGGAGGAG 
GACGAGGCGC GGGTCTACGG CGGCGTGCGA GTCGTCCCGG TGGAGCACCT CACCCCCTTC 
CCATGTGGCA TCTTCCACAA GGTGCAGGTG AACCTGTGCC GGTGGATCCA CCAGCAGAGC 
AACGAGGGGG ATGCAGACAT TCGCCTGTGG GTGAACGGCT GCAAGATCGC CAATCGCGGG 
GCCGAGCTGC TGGTGCTCCT GGTCAACCAC GGCCAGGGTA TTGAGGTCCA GGTGCGTGGA 
CTGGAGACGG AGAAGATCAA GTGCTGCTTC CTGCTGGACT CCGTGTGCAG CACCATCGAG 
AACGTCATGG CCACCACGCT GCCCGGGCTG CTGACGGTGA AGCACTACCT GAGCCCCCAG 
CAGCTACGAG AGCACCACGA GCCCGTCATG ATCTACCAGC CACGGGACTT CTTCCGGGCG 
CAGACTCTCA AGGAGACCTC ACTGACCAAC ACCATGGGCG GGTACAAGGA GAGCTTTAGC 
AGTATCATGT GCTTCGGCTG TCACGACGTC TACTCGCAGG CCAGCCTCGG CATGGACATC 
CACGCGTCAG ATCTGAACCT CCTGACCCGG AGGAAACTCA GTCGCCTGCT GGACCCGCCC 
GACCCCATGG GGAAGGACTG GTGCCTCCTT GCCATGAACT TGGGCCTCCC CGACCTTGTG 
GCCAAGTACA ACACCAATAA CGGGTCTCCC AAGGAGTTCC TCCCGAGCCC AGTCCACGCC 
CTGCTCCGGG AATGGACTTC CTACCCCGAG AGCACTGTCG GCATTCTCAT GTCCAAACTG 
CGGGAGCTGG GGCGTCGGGA CGCAGCGGAC TTCCTGCTGA AGGCGTCCTC TGTGTTCAAA 
ATCAACCTCG ACGGCAACGG CCAGGAGGCC TACGCCTCAA GCTGCAACAG TGGCACATCT 
TACAATTCCA TTAGCTCGGT CGTGTCCCGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DAPK1 ( XM_024995909.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024851677.1 Bos taurus death associated protein kinase 1 (DAPK1), transcript variant X3, mRNA.

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CloneID	OBa237231
Clone ID Related Accession (Same CDS sequence)	NM_001206038.2 , XM_024995907.1 , XM_024995909.1 , XM_024995910.1 , XM_024995908.1
Accession Version	XM_024995909.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4293bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	death-associated protein kinase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGTGT TCAGGCAGGA AAACGTGGAT GATTACTACG ACACCGGCGA GGAACTTGGC 
AGTGGGCAGT TTGCCGTCGT GAAGAAATGC CGTGAGAAAA GCACCGGTCT ACAGTATGCC 
GCCAAATTCA TCAAGAAAAG GAGGACCAAA TCCAGCCGGC GGGGCGTGAG CCGCGAGGAC 
ATCGAGCGGG AGGTCAGCAT CCTGAAGGAG ATCCAGCACC CCAACGTCAT CACCCTGCAC 
GAGGTTTACG AGAACAAGAC GGACGTCATC CTCATCCTGG AACTCGTTGC AGGTGGTGAG 
CTGTTTGACT TCTTAGCTGA AAAGGAATCT TTGACTGAAG AGGAAGCAAC TGAATTTCTC 
AAACAAATTC TTAATGGTGT TTACTATCTG CACTCCCTTC AGATTGCCCA CTTTGATCTT 
AAGCCCGAAA ACATAATGCT TTTGGATAGA AATGTTCCCA AGCCTCGGAT CAAGATCATT 
GACTTTGGGT TGGCCCATAA AATTGACTTT GGAAATGAAT TCAAAAATAT ATTTGGGACA 
CCAGAATTTG TTGCTCCTGA GATAGTCAAC TATGAACCTC TTGGTCTTGA GGCAGATATG 
TGGAGTATTG GGGTAATAAC CTATATTCTC CTCAGTGGGG CCTCCCCGTT TCTCGGAGAC 
AGTAAGCAAG AAACGTTAGC GAACGTATCT GCCGTCAACT ATGAATTTGA GGAAGAGTAC 
TTCAGTAATA CGAGCGCCCT AGCCAAAGAT TTCATAAGAA GACTCCTCGT CAAGGATCCA 
AAGAAGAGAA TGACAATTCA AGATAGTTTG CAGCATCCCT GGATCAAGCC TAAAGATACC 
CAACAAGCAC TTAGTAGAAA AGCATCAGCA GTAAACATGG AGAAATTCAA GAAGTTTGCA 
GCCCGGAAAA AATGGAAACA ATCTGTTCGC TTGATATCAC TGTGCCAAAG ATTATCCAGG 
TCATTCTTGT CCAGAAGTAA CATGAGTGTT GCCAGAAGCG ATGATACTCT GGATGAGGAA 
GACTCCTTTG TGATGAAAGC TATCATCCAT GCCATCAACG ATGACAATGT CCCAGGCCTG 
CAGCACCTCC TGGGCTCCTT GTCCAACTAC GACGTTAACC AGCCCAACAA GCATGGGACT 
CCTCCATTAC TTATTGCTGC TGGCTGTGGG AATATTCAAA TACTACAGTT GCTCATTAAG 
AGAGGCTCAA GAATTGACAT CCAGGATAAG GGTGGATCCA ATGCCATCTA CTGGGCCTCT 
CGGCATGGCC ATGTAGATAC CTTGAAATTT CTCAATGAGA ACAAATGCCC TCTGGACGTT 
AAAGACAAGT CTGGAGAGAC AGCCCTTCAT GTGGCAGCTC GCTACGGCCA CGCTGATGTG 
GTTCAGTTAC TGTGCAGCTT CGGCTCGAAT CCTAACTTCC AGGACAAGGA AGAAGAAACC 
CCCCTGCACT GTGCTGCCTG GCACGGCTAT TACTCTGTGG CCAAAGCCCT TTGTGAAGCC 
GGCTGCAATG TGAACATTAA GAACAGAGAA GGCGAGACCC CCCTCCTGAC GGCCTCCGCC 
AGGGGCTACC AAGACATCGT GGAGTGTCTG GCCGAGCACG GAGCCGACCT TAATGCATCC 
GACAAGGATG GACACATTGC TCTACATCTT GCCGTGAGAC GATGTCAGAT GGAGGTCATC 
CAGACTCTCA TCAGCCAGGG CAGTTCCGTC GATTTCCAAG ACAGGCATGG CAACACCCCC 
CTCCATGTGG CCTGCAAAGA TGGCAACGTG CCGATTGTGC TGGCCCTCTG TGAAGCCAAC 
TGCAATTTGG ATCTCTCCAA CAAGTATGGA CGGACGCCTC TGCACCTTGC CGCCAACAAC 
GGGATTCTGG ACGTGGTCCG CTACCTGTGT CTGACGGGCG CCAATGTGGA GGCGCTGACC 
TCGGATGGAA AGACAGCAGA AGATCTTGCG AAATCGGAAC AGCATGAACA CGTAGCAGGC 
CTCCTCACAA GACTCCGAAA GGACACACAC CGAGGACTCT TCATCCAGCA ACTGCGACCC 
ACACAGAACC TGCAGCCAAG AATCAAGCTG AAGCTGTTCG GCCACTCGGG GTCTGGGAAG 
ACCACTCTGG TGGAATCTCT CAAGTGTGGG CTGCTAAGGA GCTTCTTCCG AAGACGCCGG 
CCCCGGCTCT CCTCCACCAA CTCTACCCGG TTCCCACCCT CGCCCCTGGC CTCCAAGCCG 
GCAGTGTCCG TGAGCATCAG CAACCTGTAC CCGGGCTGTG AGAACGTGAG TGTCAGGAGC 
CGCAGCATGA TGTTCGAGCC AGGCCTCACC AAGGGGGTGC TGGAGGTGTT CGGGGCCCCG 
TCCCACCATC CGCACTGCGC CACCGATGAC CAGTCCACGA AGGCAATCGA CATCCAGAAT 
GCCTATTTGA ACGGCATTGG TGACTTCAGT GTGTGGGAGT TCTCTGGAAA TCCTGTCTAC 
TTCTGCTGTT ATGACTATTT TGCTGCAAAC GATCCCACGT CCATCCATGT CATCGTTTTC 
AGTCTAGAAG AACCCTATGA GATTCAGCTG AATCAAGTGA TTTTCTGGCT TAGTTTCCTG 
AAGTCTCTTG TCCCAGTGGA AGAACCGATA GCCTTTGGCG GCAAGCTGAA GAACCCACTC 
CGAGTTGTCC TGGTGGCCAC CCATGCCGAC ATCGTGAACC TTCCTCGTTC AGCTGGAGGC 
GAGTTTGGAT ATGACAAAGA CACCTCGTTG CTGAAAGAGA TCAGGAACAG GTTTGGGAAC 
GATCTTCACA TTTCAAATAA GCTCTTTGTT CTGGATGCCG GGGCTTCTGG GTCTAAGGAC 
ATGAAGGTGC TTCGAAATCA CCTGCAAGAA ATACGGAGCC AGATTGTTTC GGTCTGCCCT 
CCAATGACTC ACCTATGTGA AAAGATCATC TCCACTTTGC CCTCCTGGAG GAAGCTCAAT 
GGCCCCAACC AGCTGATGTC ACTGCAGCAG TTCGTGTACG ACGTACAGGA CCAGTTGAAC 
CCCCTGGCCA GCGAGGCAGA CCTCCGGCAC ATCGCCCAGC AGCTCCACAG TGCTGGCGAG 
ATCAACATCA TGCAGAGCGA GACCGTCCAG GACGTGCTGC TCCTGGATCC CCGCTGGCTG 
TGCACCAACG TTCTGGGGAA GCTGCTCTCT GTGGAGACCC CACGGGCCCT GCACCATTAC 
CGCGGCCGCT ACACCATGGA GGACGTCCAG CGCCTGGTGC CCGACAGTGA CGTGGAGGAG 
CTGCTGCAGA TCCTGGATGC CATGGACATC TGTGCACGGG ACCTGAGCAG CGGGACCATG 
GTGGACATCC CCGCCCTGAT CAAGACTGAC AGCCTACACC GCTCCTGGAC GGAGGAGGAG 
GACGAGGCGC GGGTCTACGG CGGCGTGCGA GTCGTCCCGG TGGAGCACCT CACCCCCTTC 
CCATGTGGCA TCTTCCACAA GGTGCAGGTG AACCTGTGCC GGTGGATCCA CCAGCAGAGC 
AACGAGGGGG ATGCAGACAT TCGCCTGTGG GTGAACGGCT GCAAGATCGC CAATCGCGGG 
GCCGAGCTGC TGGTGCTCCT GGTCAACCAC GGCCAGGGTA TTGAGGTCCA GGTGCGTGGA 
CTGGAGACGG AGAAGATCAA GTGCTGCTTC CTGCTGGACT CCGTGTGCAG CACCATCGAG 
AACGTCATGG CCACCACGCT GCCCGGGCTG CTGACGGTGA AGCACTACCT GAGCCCCCAG 
CAGCTACGAG AGCACCACGA GCCCGTCATG ATCTACCAGC CACGGGACTT CTTCCGGGCG 
CAGACTCTCA AGGAGACCTC ACTGACCAAC ACCATGGGCG GGTACAAGGA GAGCTTTAGC 
AGTATCATGT GCTTCGGCTG TCACGACGTC TACTCGCAGG CCAGCCTCGG CATGGACATC 
CACGCGTCAG ATCTGAACCT CCTGACCCGG AGGAAACTCA GTCGCCTGCT GGACCCGCCC 
GACCCCATGG GGAAGGACTG GTGCCTCCTT GCCATGAACT TGGGCCTCCC CGACCTTGTG 
GCCAAGTACA ACACCAATAA CGGGTCTCCC AAGGAGTTCC TCCCGAGCCC AGTCCACGCC 
CTGCTCCGGG AATGGACTTC CTACCCCGAG AGCACTGTCG GCATTCTCAT GTCCAAACTG 
CGGGAGCTGG GGCGTCGGGA CGCAGCGGAC TTCCTGCTGA AGGCGTCCTC TGTGTTCAAA 
ATCAACCTCG ACGGCAACGG CCAGGAGGCC TACGCCTCAA GCTGCAACAG TGGCACATCT 
TACAATTCCA TTAGCTCGGT CGTGTCCCGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024851677.1
CDS	242..4534
Translation	MTVFRQENVDDYYDTGEELGSGQFAVVKKCREKSTGLQYAAKFIKKRRTKSSRRGVSREDIEREVSILKEIQHPNVITLHEVYENKTDVILILELVAGGELFDFLAEKESLTEEEATEFLKQILNGVYYLHSLQIAHFDLKPENIMLLDRNVPKPRIKIIDFGLAHKIDFGNEFKNIFGTPEFVAPEIVNYEPLGLEADMWSIGVITYILLSGASPFLGDSKQETLANVSAVNYEFEEEYFSNTSALAKDFIRRLLVKDPKKRMTIQDSLQHPWIKPKDTQQALSRKASAVNMEKFKKFAARKKWKQSVRLISLCQRLSRSFLSRSNMSVARSDDTLDEEDSFVMKAIIHAINDDNVPGLQHLLGSLSNYDVNQPNKHGTPPLLIAAGCGNIQILQLLIKRGSRIDIQDKGGSNAIYWASRHGHVDTLKFLNENKCPLDVKDKSGETALHVAARYGHADVVQLLCSFGSNPNFQDKEEETPLHCAAWHGYYSVAKALCEAGCNVNIKNREGETPLLTASARGYQDIVECLAEHGADLNASDKDGHIALHLAVRRCQMEVIQTLISQGSSVDFQDRHGNTPLHVACKDGNVPIVLALCEANCNLDLSNKYGRTPLHLAANNGILDVVRYLCLTGANVEALTSDGKTAEDLAKSEQHEHVAGLLTRLRKDTHRGLFIQQLRPTQNLQPRIKLKLFGHSGSGKTTLVESLKCGLLRSFFRRRRPRLSSTNSTRFPPSPLASKPAVSVSISNLYPGCENVSVRSRSMMFEPGLTKGVLEVFGAPSHHPHCATDDQSTKAIDIQNAYLNGIGDFSVWEFSGNPVYFCCYDYFAANDPTSIHVIVFSLEEPYEIQLNQVIFWLSFLKSLVPVEEPIAFGGKLKNPLRVVLVATHADIVNLPRSAGGEFGYDKDTSLLKEIRNRFGNDLHISNKLFVLDAGASGSKDMKVLRNHLQEIRSQIVSVCPPMTHLCEKIISTLPSWRKLNGPNQLMSLQQFVYDVQDQLNPLASEADLRHIAQQLHSAGEINIMQSETVQDVLLLDPRWLCTNVLGKLLSVETPRALHHYRGRYTMEDVQRLVPDSDVEELLQILDAMDICARDLSSGTMVDIPALIKTDSLHRSWTEEEDEARVYGGVRVVPVEHLTPFPCGIFHKVQVNLCRWIHQQSNEGDADIRLWVNGCKIANRGAELLVLLVNHGQGIEVQVRGLETEKIKCCFLLDSVCSTIENVMATTLPGLLTVKHYLSPQQLREHHEPVMIYQPRDFFRAQTLKETSLTNTMGGYKESFSSIMCFGCHDVYSQASLGMDIHASDLNLLTRRKLSRLLDPPDPMGKDWCLLAMNLGLPDLVAKYNTNNGSPKEFLPSPVHALLREWTSYPESTVGILMSKLRELGRRDAADFLLKASSVFKINLDGNGQEAYASSCNSGTSYNSISSVVSR

Target ORF information:

RefSeq Version	XM_024995909.1
Organism	Bos taurus(cattle)
Definition	Bos taurus death associated protein kinase 1 (DAPK1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024995909.1

ORF Insert Sequence:

ATGACCGTGT TCAGGCAGGA AAACGTGGAT GATTACTACG ACACCGGCGA GGAACTTGGC 
AGTGGGCAGT TTGCCGTCGT GAAGAAATGC CGTGAGAAAA GCACCGGTCT ACAGTATGCC 
GCCAAATTCA TCAAGAAAAG GAGGACCAAA TCCAGCCGGC GGGGCGTGAG CCGCGAGGAC 
ATCGAGCGGG AGGTCAGCAT CCTGAAGGAG ATCCAGCACC CCAACGTCAT CACCCTGCAC 
GAGGTTTACG AGAACAAGAC GGACGTCATC CTCATCCTGG AACTCGTTGC AGGTGGTGAG 
CTGTTTGACT TCTTAGCTGA AAAGGAATCT TTGACTGAAG AGGAAGCAAC TGAATTTCTC 
AAACAAATTC TTAATGGTGT TTACTATCTG CACTCCCTTC AGATTGCCCA CTTTGATCTT 
AAGCCCGAAA ACATAATGCT TTTGGATAGA AATGTTCCCA AGCCTCGGAT CAAGATCATT 
GACTTTGGGT TGGCCCATAA AATTGACTTT GGAAATGAAT TCAAAAATAT ATTTGGGACA 
CCAGAATTTG TTGCTCCTGA GATAGTCAAC TATGAACCTC TTGGTCTTGA GGCAGATATG 
TGGAGTATTG GGGTAATAAC CTATATTCTC CTCAGTGGGG CCTCCCCGTT TCTCGGAGAC 
AGTAAGCAAG AAACGTTAGC GAACGTATCT GCCGTCAACT ATGAATTTGA GGAAGAGTAC 
TTCAGTAATA CGAGCGCCCT AGCCAAAGAT TTCATAAGAA GACTCCTCGT CAAGGATCCA 
AAGAAGAGAA TGACAATTCA AGATAGTTTG CAGCATCCCT GGATCAAGCC TAAAGATACC 
CAACAAGCAC TTAGTAGAAA AGCATCAGCA GTAAACATGG AGAAATTCAA GAAGTTTGCA 
GCCCGGAAAA AATGGAAACA ATCTGTTCGC TTGATATCAC TGTGCCAAAG ATTATCCAGG 
TCATTCTTGT CCAGAAGTAA CATGAGTGTT GCCAGAAGCG ATGATACTCT GGATGAGGAA 
GACTCCTTTG TGATGAAAGC TATCATCCAT GCCATCAACG ATGACAATGT CCCAGGCCTG 
CAGCACCTCC TGGGCTCCTT GTCCAACTAC GACGTTAACC AGCCCAACAA GCATGGGACT 
CCTCCATTAC TTATTGCTGC TGGCTGTGGG AATATTCAAA TACTACAGTT GCTCATTAAG 
AGAGGCTCAA GAATTGACAT CCAGGATAAG GGTGGATCCA ATGCCATCTA CTGGGCCTCT 
CGGCATGGCC ATGTAGATAC CTTGAAATTT CTCAATGAGA ACAAATGCCC TCTGGACGTT 
AAAGACAAGT CTGGAGAGAC AGCCCTTCAT GTGGCAGCTC GCTACGGCCA CGCTGATGTG 
GTTCAGTTAC TGTGCAGCTT CGGCTCGAAT CCTAACTTCC AGGACAAGGA AGAAGAAACC 
CCCCTGCACT GTGCTGCCTG GCACGGCTAT TACTCTGTGG CCAAAGCCCT TTGTGAAGCC 
GGCTGCAATG TGAACATTAA GAACAGAGAA GGCGAGACCC CCCTCCTGAC GGCCTCCGCC 
AGGGGCTACC AAGACATCGT GGAGTGTCTG GCCGAGCACG GAGCCGACCT TAATGCATCC 
GACAAGGATG GACACATTGC TCTACATCTT GCCGTGAGAC GATGTCAGAT GGAGGTCATC 
CAGACTCTCA TCAGCCAGGG CAGTTCCGTC GATTTCCAAG ACAGGCATGG CAACACCCCC 
CTCCATGTGG CCTGCAAAGA TGGCAACGTG CCGATTGTGC TGGCCCTCTG TGAAGCCAAC 
TGCAATTTGG ATCTCTCCAA CAAGTATGGA CGGACGCCTC TGCACCTTGC CGCCAACAAC 
GGGATTCTGG ACGTGGTCCG CTACCTGTGT CTGACGGGCG CCAATGTGGA GGCGCTGACC 
TCGGATGGAA AGACAGCAGA AGATCTTGCG AAATCGGAAC AGCATGAACA CGTAGCAGGC 
CTCCTCACAA GACTCCGAAA GGACACACAC CGAGGACTCT TCATCCAGCA ACTGCGACCC 
ACACAGAACC TGCAGCCAAG AATCAAGCTG AAGCTGTTCG GCCACTCGGG GTCTGGGAAG 
ACCACTCTGG TGGAATCTCT CAAGTGTGGG CTGCTAAGGA GCTTCTTCCG AAGACGCCGG 
CCCCGGCTCT CCTCCACCAA CTCTACCCGG TTCCCACCCT CGCCCCTGGC CTCCAAGCCG 
GCAGTGTCCG TGAGCATCAG CAACCTGTAC CCGGGCTGTG AGAACGTGAG TGTCAGGAGC 
CGCAGCATGA TGTTCGAGCC AGGCCTCACC AAGGGGGTGC TGGAGGTGTT CGGGGCCCCG 
TCCCACCATC CGCACTGCGC CACCGATGAC CAGTCCACGA AGGCAATCGA CATCCAGAAT 
GCCTATTTGA ACGGCATTGG TGACTTCAGT GTGTGGGAGT TCTCTGGAAA TCCTGTCTAC 
TTCTGCTGTT ATGACTATTT TGCTGCAAAC GATCCCACGT CCATCCATGT CATCGTTTTC 
AGTCTAGAAG AACCCTATGA GATTCAGCTG AATCAAGTGA TTTTCTGGCT TAGTTTCCTG 
AAGTCTCTTG TCCCAGTGGA AGAACCGATA GCCTTTGGCG GCAAGCTGAA GAACCCACTC 
CGAGTTGTCC TGGTGGCCAC CCATGCCGAC ATCGTGAACC TTCCTCGTTC AGCTGGAGGC 
GAGTTTGGAT ATGACAAAGA CACCTCGTTG CTGAAAGAGA TCAGGAACAG GTTTGGGAAC 
GATCTTCACA TTTCAAATAA GCTCTTTGTT CTGGATGCCG GGGCTTCTGG GTCTAAGGAC 
ATGAAGGTGC TTCGAAATCA CCTGCAAGAA ATACGGAGCC AGATTGTTTC GGTCTGCCCT 
CCAATGACTC ACCTATGTGA AAAGATCATC TCCACTTTGC CCTCCTGGAG GAAGCTCAAT 
GGCCCCAACC AGCTGATGTC ACTGCAGCAG TTCGTGTACG ACGTACAGGA CCAGTTGAAC 
CCCCTGGCCA GCGAGGCAGA CCTCCGGCAC ATCGCCCAGC AGCTCCACAG TGCTGGCGAG 
ATCAACATCA TGCAGAGCGA GACCGTCCAG GACGTGCTGC TCCTGGATCC CCGCTGGCTG 
TGCACCAACG TTCTGGGGAA GCTGCTCTCT GTGGAGACCC CACGGGCCCT GCACCATTAC 
CGCGGCCGCT ACACCATGGA GGACGTCCAG CGCCTGGTGC CCGACAGTGA CGTGGAGGAG 
CTGCTGCAGA TCCTGGATGC CATGGACATC TGTGCACGGG ACCTGAGCAG CGGGACCATG 
GTGGACATCC CCGCCCTGAT CAAGACTGAC AGCCTACACC GCTCCTGGAC GGAGGAGGAG 
GACGAGGCGC GGGTCTACGG CGGCGTGCGA GTCGTCCCGG TGGAGCACCT CACCCCCTTC 
CCATGTGGCA TCTTCCACAA GGTGCAGGTG AACCTGTGCC GGTGGATCCA CCAGCAGAGC 
AACGAGGGGG ATGCAGACAT TCGCCTGTGG GTGAACGGCT GCAAGATCGC CAATCGCGGG 
GCCGAGCTGC TGGTGCTCCT GGTCAACCAC GGCCAGGGTA TTGAGGTCCA GGTGCGTGGA 
CTGGAGACGG AGAAGATCAA GTGCTGCTTC CTGCTGGACT CCGTGTGCAG CACCATCGAG 
AACGTCATGG CCACCACGCT GCCCGGGCTG CTGACGGTGA AGCACTACCT GAGCCCCCAG 
CAGCTACGAG AGCACCACGA GCCCGTCATG ATCTACCAGC CACGGGACTT CTTCCGGGCG 
CAGACTCTCA AGGAGACCTC ACTGACCAAC ACCATGGGCG GGTACAAGGA GAGCTTTAGC 
AGTATCATGT GCTTCGGCTG TCACGACGTC TACTCGCAGG CCAGCCTCGG CATGGACATC 
CACGCGTCAG ATCTGAACCT CCTGACCCGG AGGAAACTCA GTCGCCTGCT GGACCCGCCC 
GACCCCATGG GGAAGGACTG GTGCCTCCTT GCCATGAACT TGGGCCTCCC CGACCTTGTG 
GCCAAGTACA ACACCAATAA CGGGTCTCCC AAGGAGTTCC TCCCGAGCCC AGTCCACGCC 
CTGCTCCGGG AATGGACTTC CTACCCCGAG AGCACTGTCG GCATTCTCAT GTCCAAACTG 
CGGGAGCTGG GGCGTCGGGA CGCAGCGGAC TTCCTGCTGA AGGCGTCCTC TGTGTTCAAA 
ATCAACCTCG ACGGCAACGG CCAGGAGGCC TACGCCTCAA GCTGCAACAG TGGCACATCT 
TACAATTCCA TTAGCTCGGT CGTGTCCCGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DAPK1 ( XM_024995910.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024851678.1 Bos taurus death associated protein kinase 1 (DAPK1), transcript variant X4, mRNA.

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CloneID	OBa237231
Clone ID Related Accession (Same CDS sequence)	NM_001206038.2 , XM_024995907.1 , XM_024995909.1 , XM_024995910.1 , XM_024995908.1
Accession Version	XM_024995910.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4293bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	death-associated protein kinase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGTGT TCAGGCAGGA AAACGTGGAT GATTACTACG ACACCGGCGA GGAACTTGGC 
AGTGGGCAGT TTGCCGTCGT GAAGAAATGC CGTGAGAAAA GCACCGGTCT ACAGTATGCC 
GCCAAATTCA TCAAGAAAAG GAGGACCAAA TCCAGCCGGC GGGGCGTGAG CCGCGAGGAC 
ATCGAGCGGG AGGTCAGCAT CCTGAAGGAG ATCCAGCACC CCAACGTCAT CACCCTGCAC 
GAGGTTTACG AGAACAAGAC GGACGTCATC CTCATCCTGG AACTCGTTGC AGGTGGTGAG 
CTGTTTGACT TCTTAGCTGA AAAGGAATCT TTGACTGAAG AGGAAGCAAC TGAATTTCTC 
AAACAAATTC TTAATGGTGT TTACTATCTG CACTCCCTTC AGATTGCCCA CTTTGATCTT 
AAGCCCGAAA ACATAATGCT TTTGGATAGA AATGTTCCCA AGCCTCGGAT CAAGATCATT 
GACTTTGGGT TGGCCCATAA AATTGACTTT GGAAATGAAT TCAAAAATAT ATTTGGGACA 
CCAGAATTTG TTGCTCCTGA GATAGTCAAC TATGAACCTC TTGGTCTTGA GGCAGATATG 
TGGAGTATTG GGGTAATAAC CTATATTCTC CTCAGTGGGG CCTCCCCGTT TCTCGGAGAC 
AGTAAGCAAG AAACGTTAGC GAACGTATCT GCCGTCAACT ATGAATTTGA GGAAGAGTAC 
TTCAGTAATA CGAGCGCCCT AGCCAAAGAT TTCATAAGAA GACTCCTCGT CAAGGATCCA 
AAGAAGAGAA TGACAATTCA AGATAGTTTG CAGCATCCCT GGATCAAGCC TAAAGATACC 
CAACAAGCAC TTAGTAGAAA AGCATCAGCA GTAAACATGG AGAAATTCAA GAAGTTTGCA 
GCCCGGAAAA AATGGAAACA ATCTGTTCGC TTGATATCAC TGTGCCAAAG ATTATCCAGG 
TCATTCTTGT CCAGAAGTAA CATGAGTGTT GCCAGAAGCG ATGATACTCT GGATGAGGAA 
GACTCCTTTG TGATGAAAGC TATCATCCAT GCCATCAACG ATGACAATGT CCCAGGCCTG 
CAGCACCTCC TGGGCTCCTT GTCCAACTAC GACGTTAACC AGCCCAACAA GCATGGGACT 
CCTCCATTAC TTATTGCTGC TGGCTGTGGG AATATTCAAA TACTACAGTT GCTCATTAAG 
AGAGGCTCAA GAATTGACAT CCAGGATAAG GGTGGATCCA ATGCCATCTA CTGGGCCTCT 
CGGCATGGCC ATGTAGATAC CTTGAAATTT CTCAATGAGA ACAAATGCCC TCTGGACGTT 
AAAGACAAGT CTGGAGAGAC AGCCCTTCAT GTGGCAGCTC GCTACGGCCA CGCTGATGTG 
GTTCAGTTAC TGTGCAGCTT CGGCTCGAAT CCTAACTTCC AGGACAAGGA AGAAGAAACC 
CCCCTGCACT GTGCTGCCTG GCACGGCTAT TACTCTGTGG CCAAAGCCCT TTGTGAAGCC 
GGCTGCAATG TGAACATTAA GAACAGAGAA GGCGAGACCC CCCTCCTGAC GGCCTCCGCC 
AGGGGCTACC AAGACATCGT GGAGTGTCTG GCCGAGCACG GAGCCGACCT TAATGCATCC 
GACAAGGATG GACACATTGC TCTACATCTT GCCGTGAGAC GATGTCAGAT GGAGGTCATC 
CAGACTCTCA TCAGCCAGGG CAGTTCCGTC GATTTCCAAG ACAGGCATGG CAACACCCCC 
CTCCATGTGG CCTGCAAAGA TGGCAACGTG CCGATTGTGC TGGCCCTCTG TGAAGCCAAC 
TGCAATTTGG ATCTCTCCAA CAAGTATGGA CGGACGCCTC TGCACCTTGC CGCCAACAAC 
GGGATTCTGG ACGTGGTCCG CTACCTGTGT CTGACGGGCG CCAATGTGGA GGCGCTGACC 
TCGGATGGAA AGACAGCAGA AGATCTTGCG AAATCGGAAC AGCATGAACA CGTAGCAGGC 
CTCCTCACAA GACTCCGAAA GGACACACAC CGAGGACTCT TCATCCAGCA ACTGCGACCC 
ACACAGAACC TGCAGCCAAG AATCAAGCTG AAGCTGTTCG GCCACTCGGG GTCTGGGAAG 
ACCACTCTGG TGGAATCTCT CAAGTGTGGG CTGCTAAGGA GCTTCTTCCG AAGACGCCGG 
CCCCGGCTCT CCTCCACCAA CTCTACCCGG TTCCCACCCT CGCCCCTGGC CTCCAAGCCG 
GCAGTGTCCG TGAGCATCAG CAACCTGTAC CCGGGCTGTG AGAACGTGAG TGTCAGGAGC 
CGCAGCATGA TGTTCGAGCC AGGCCTCACC AAGGGGGTGC TGGAGGTGTT CGGGGCCCCG 
TCCCACCATC CGCACTGCGC CACCGATGAC CAGTCCACGA AGGCAATCGA CATCCAGAAT 
GCCTATTTGA ACGGCATTGG TGACTTCAGT GTGTGGGAGT TCTCTGGAAA TCCTGTCTAC 
TTCTGCTGTT ATGACTATTT TGCTGCAAAC GATCCCACGT CCATCCATGT CATCGTTTTC 
AGTCTAGAAG AACCCTATGA GATTCAGCTG AATCAAGTGA TTTTCTGGCT TAGTTTCCTG 
AAGTCTCTTG TCCCAGTGGA AGAACCGATA GCCTTTGGCG GCAAGCTGAA GAACCCACTC 
CGAGTTGTCC TGGTGGCCAC CCATGCCGAC ATCGTGAACC TTCCTCGTTC AGCTGGAGGC 
GAGTTTGGAT ATGACAAAGA CACCTCGTTG CTGAAAGAGA TCAGGAACAG GTTTGGGAAC 
GATCTTCACA TTTCAAATAA GCTCTTTGTT CTGGATGCCG GGGCTTCTGG GTCTAAGGAC 
ATGAAGGTGC TTCGAAATCA CCTGCAAGAA ATACGGAGCC AGATTGTTTC GGTCTGCCCT 
CCAATGACTC ACCTATGTGA AAAGATCATC TCCACTTTGC CCTCCTGGAG GAAGCTCAAT 
GGCCCCAACC AGCTGATGTC ACTGCAGCAG TTCGTGTACG ACGTACAGGA CCAGTTGAAC 
CCCCTGGCCA GCGAGGCAGA CCTCCGGCAC ATCGCCCAGC AGCTCCACAG TGCTGGCGAG 
ATCAACATCA TGCAGAGCGA GACCGTCCAG GACGTGCTGC TCCTGGATCC CCGCTGGCTG 
TGCACCAACG TTCTGGGGAA GCTGCTCTCT GTGGAGACCC CACGGGCCCT GCACCATTAC 
CGCGGCCGCT ACACCATGGA GGACGTCCAG CGCCTGGTGC CCGACAGTGA CGTGGAGGAG 
CTGCTGCAGA TCCTGGATGC CATGGACATC TGTGCACGGG ACCTGAGCAG CGGGACCATG 
GTGGACATCC CCGCCCTGAT CAAGACTGAC AGCCTACACC GCTCCTGGAC GGAGGAGGAG 
GACGAGGCGC GGGTCTACGG CGGCGTGCGA GTCGTCCCGG TGGAGCACCT CACCCCCTTC 
CCATGTGGCA TCTTCCACAA GGTGCAGGTG AACCTGTGCC GGTGGATCCA CCAGCAGAGC 
AACGAGGGGG ATGCAGACAT TCGCCTGTGG GTGAACGGCT GCAAGATCGC CAATCGCGGG 
GCCGAGCTGC TGGTGCTCCT GGTCAACCAC GGCCAGGGTA TTGAGGTCCA GGTGCGTGGA 
CTGGAGACGG AGAAGATCAA GTGCTGCTTC CTGCTGGACT CCGTGTGCAG CACCATCGAG 
AACGTCATGG CCACCACGCT GCCCGGGCTG CTGACGGTGA AGCACTACCT GAGCCCCCAG 
CAGCTACGAG AGCACCACGA GCCCGTCATG ATCTACCAGC CACGGGACTT CTTCCGGGCG 
CAGACTCTCA AGGAGACCTC ACTGACCAAC ACCATGGGCG GGTACAAGGA GAGCTTTAGC 
AGTATCATGT GCTTCGGCTG TCACGACGTC TACTCGCAGG CCAGCCTCGG CATGGACATC 
CACGCGTCAG ATCTGAACCT CCTGACCCGG AGGAAACTCA GTCGCCTGCT GGACCCGCCC 
GACCCCATGG GGAAGGACTG GTGCCTCCTT GCCATGAACT TGGGCCTCCC CGACCTTGTG 
GCCAAGTACA ACACCAATAA CGGGTCTCCC AAGGAGTTCC TCCCGAGCCC AGTCCACGCC 
CTGCTCCGGG AATGGACTTC CTACCCCGAG AGCACTGTCG GCATTCTCAT GTCCAAACTG 
CGGGAGCTGG GGCGTCGGGA CGCAGCGGAC TTCCTGCTGA AGGCGTCCTC TGTGTTCAAA 
ATCAACCTCG ACGGCAACGG CCAGGAGGCC TACGCCTCAA GCTGCAACAG TGGCACATCT 
TACAATTCCA TTAGCTCGGT CGTGTCCCGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024851678.1
CDS	295..4587
Translation	MTVFRQENVDDYYDTGEELGSGQFAVVKKCREKSTGLQYAAKFIKKRRTKSSRRGVSREDIEREVSILKEIQHPNVITLHEVYENKTDVILILELVAGGELFDFLAEKESLTEEEATEFLKQILNGVYYLHSLQIAHFDLKPENIMLLDRNVPKPRIKIIDFGLAHKIDFGNEFKNIFGTPEFVAPEIVNYEPLGLEADMWSIGVITYILLSGASPFLGDSKQETLANVSAVNYEFEEEYFSNTSALAKDFIRRLLVKDPKKRMTIQDSLQHPWIKPKDTQQALSRKASAVNMEKFKKFAARKKWKQSVRLISLCQRLSRSFLSRSNMSVARSDDTLDEEDSFVMKAIIHAINDDNVPGLQHLLGSLSNYDVNQPNKHGTPPLLIAAGCGNIQILQLLIKRGSRIDIQDKGGSNAIYWASRHGHVDTLKFLNENKCPLDVKDKSGETALHVAARYGHADVVQLLCSFGSNPNFQDKEEETPLHCAAWHGYYSVAKALCEAGCNVNIKNREGETPLLTASARGYQDIVECLAEHGADLNASDKDGHIALHLAVRRCQMEVIQTLISQGSSVDFQDRHGNTPLHVACKDGNVPIVLALCEANCNLDLSNKYGRTPLHLAANNGILDVVRYLCLTGANVEALTSDGKTAEDLAKSEQHEHVAGLLTRLRKDTHRGLFIQQLRPTQNLQPRIKLKLFGHSGSGKTTLVESLKCGLLRSFFRRRRPRLSSTNSTRFPPSPLASKPAVSVSISNLYPGCENVSVRSRSMMFEPGLTKGVLEVFGAPSHHPHCATDDQSTKAIDIQNAYLNGIGDFSVWEFSGNPVYFCCYDYFAANDPTSIHVIVFSLEEPYEIQLNQVIFWLSFLKSLVPVEEPIAFGGKLKNPLRVVLVATHADIVNLPRSAGGEFGYDKDTSLLKEIRNRFGNDLHISNKLFVLDAGASGSKDMKVLRNHLQEIRSQIVSVCPPMTHLCEKIISTLPSWRKLNGPNQLMSLQQFVYDVQDQLNPLASEADLRHIAQQLHSAGEINIMQSETVQDVLLLDPRWLCTNVLGKLLSVETPRALHHYRGRYTMEDVQRLVPDSDVEELLQILDAMDICARDLSSGTMVDIPALIKTDSLHRSWTEEEDEARVYGGVRVVPVEHLTPFPCGIFHKVQVNLCRWIHQQSNEGDADIRLWVNGCKIANRGAELLVLLVNHGQGIEVQVRGLETEKIKCCFLLDSVCSTIENVMATTLPGLLTVKHYLSPQQLREHHEPVMIYQPRDFFRAQTLKETSLTNTMGGYKESFSSIMCFGCHDVYSQASLGMDIHASDLNLLTRRKLSRLLDPPDPMGKDWCLLAMNLGLPDLVAKYNTNNGSPKEFLPSPVHALLREWTSYPESTVGILMSKLRELGRRDAADFLLKASSVFKINLDGNGQEAYASSCNSGTSYNSISSVVSR

Target ORF information:

RefSeq Version	XM_024995910.1
Organism	Bos taurus(cattle)
Definition	Bos taurus death associated protein kinase 1 (DAPK1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024995910.1

ORF Insert Sequence:

ATGACCGTGT TCAGGCAGGA AAACGTGGAT GATTACTACG ACACCGGCGA GGAACTTGGC 
AGTGGGCAGT TTGCCGTCGT GAAGAAATGC CGTGAGAAAA GCACCGGTCT ACAGTATGCC 
GCCAAATTCA TCAAGAAAAG GAGGACCAAA TCCAGCCGGC GGGGCGTGAG CCGCGAGGAC 
ATCGAGCGGG AGGTCAGCAT CCTGAAGGAG ATCCAGCACC CCAACGTCAT CACCCTGCAC 
GAGGTTTACG AGAACAAGAC GGACGTCATC CTCATCCTGG AACTCGTTGC AGGTGGTGAG 
CTGTTTGACT TCTTAGCTGA AAAGGAATCT TTGACTGAAG AGGAAGCAAC TGAATTTCTC 
AAACAAATTC TTAATGGTGT TTACTATCTG CACTCCCTTC AGATTGCCCA CTTTGATCTT 
AAGCCCGAAA ACATAATGCT TTTGGATAGA AATGTTCCCA AGCCTCGGAT CAAGATCATT 
GACTTTGGGT TGGCCCATAA AATTGACTTT GGAAATGAAT TCAAAAATAT ATTTGGGACA 
CCAGAATTTG TTGCTCCTGA GATAGTCAAC TATGAACCTC TTGGTCTTGA GGCAGATATG 
TGGAGTATTG GGGTAATAAC CTATATTCTC CTCAGTGGGG CCTCCCCGTT TCTCGGAGAC 
AGTAAGCAAG AAACGTTAGC GAACGTATCT GCCGTCAACT ATGAATTTGA GGAAGAGTAC 
TTCAGTAATA CGAGCGCCCT AGCCAAAGAT TTCATAAGAA GACTCCTCGT CAAGGATCCA 
AAGAAGAGAA TGACAATTCA AGATAGTTTG CAGCATCCCT GGATCAAGCC TAAAGATACC 
CAACAAGCAC TTAGTAGAAA AGCATCAGCA GTAAACATGG AGAAATTCAA GAAGTTTGCA 
GCCCGGAAAA AATGGAAACA ATCTGTTCGC TTGATATCAC TGTGCCAAAG ATTATCCAGG 
TCATTCTTGT CCAGAAGTAA CATGAGTGTT GCCAGAAGCG ATGATACTCT GGATGAGGAA 
GACTCCTTTG TGATGAAAGC TATCATCCAT GCCATCAACG ATGACAATGT CCCAGGCCTG 
CAGCACCTCC TGGGCTCCTT GTCCAACTAC GACGTTAACC AGCCCAACAA GCATGGGACT 
CCTCCATTAC TTATTGCTGC TGGCTGTGGG AATATTCAAA TACTACAGTT GCTCATTAAG 
AGAGGCTCAA GAATTGACAT CCAGGATAAG GGTGGATCCA ATGCCATCTA CTGGGCCTCT 
CGGCATGGCC ATGTAGATAC CTTGAAATTT CTCAATGAGA ACAAATGCCC TCTGGACGTT 
AAAGACAAGT CTGGAGAGAC AGCCCTTCAT GTGGCAGCTC GCTACGGCCA CGCTGATGTG 
GTTCAGTTAC TGTGCAGCTT CGGCTCGAAT CCTAACTTCC AGGACAAGGA AGAAGAAACC 
CCCCTGCACT GTGCTGCCTG GCACGGCTAT TACTCTGTGG CCAAAGCCCT TTGTGAAGCC 
GGCTGCAATG TGAACATTAA GAACAGAGAA GGCGAGACCC CCCTCCTGAC GGCCTCCGCC 
AGGGGCTACC AAGACATCGT GGAGTGTCTG GCCGAGCACG GAGCCGACCT TAATGCATCC 
GACAAGGATG GACACATTGC TCTACATCTT GCCGTGAGAC GATGTCAGAT GGAGGTCATC 
CAGACTCTCA TCAGCCAGGG CAGTTCCGTC GATTTCCAAG ACAGGCATGG CAACACCCCC 
CTCCATGTGG CCTGCAAAGA TGGCAACGTG CCGATTGTGC TGGCCCTCTG TGAAGCCAAC 
TGCAATTTGG ATCTCTCCAA CAAGTATGGA CGGACGCCTC TGCACCTTGC CGCCAACAAC 
GGGATTCTGG ACGTGGTCCG CTACCTGTGT CTGACGGGCG CCAATGTGGA GGCGCTGACC 
TCGGATGGAA AGACAGCAGA AGATCTTGCG AAATCGGAAC AGCATGAACA CGTAGCAGGC 
CTCCTCACAA GACTCCGAAA GGACACACAC CGAGGACTCT TCATCCAGCA ACTGCGACCC 
ACACAGAACC TGCAGCCAAG AATCAAGCTG AAGCTGTTCG GCCACTCGGG GTCTGGGAAG 
ACCACTCTGG TGGAATCTCT CAAGTGTGGG CTGCTAAGGA GCTTCTTCCG AAGACGCCGG 
CCCCGGCTCT CCTCCACCAA CTCTACCCGG TTCCCACCCT CGCCCCTGGC CTCCAAGCCG 
GCAGTGTCCG TGAGCATCAG CAACCTGTAC CCGGGCTGTG AGAACGTGAG TGTCAGGAGC 
CGCAGCATGA TGTTCGAGCC AGGCCTCACC AAGGGGGTGC TGGAGGTGTT CGGGGCCCCG 
TCCCACCATC CGCACTGCGC CACCGATGAC CAGTCCACGA AGGCAATCGA CATCCAGAAT 
GCCTATTTGA ACGGCATTGG TGACTTCAGT GTGTGGGAGT TCTCTGGAAA TCCTGTCTAC 
TTCTGCTGTT ATGACTATTT TGCTGCAAAC GATCCCACGT CCATCCATGT CATCGTTTTC 
AGTCTAGAAG AACCCTATGA GATTCAGCTG AATCAAGTGA TTTTCTGGCT TAGTTTCCTG 
AAGTCTCTTG TCCCAGTGGA AGAACCGATA GCCTTTGGCG GCAAGCTGAA GAACCCACTC 
CGAGTTGTCC TGGTGGCCAC CCATGCCGAC ATCGTGAACC TTCCTCGTTC AGCTGGAGGC 
GAGTTTGGAT ATGACAAAGA CACCTCGTTG CTGAAAGAGA TCAGGAACAG GTTTGGGAAC 
GATCTTCACA TTTCAAATAA GCTCTTTGTT CTGGATGCCG GGGCTTCTGG GTCTAAGGAC 
ATGAAGGTGC TTCGAAATCA CCTGCAAGAA ATACGGAGCC AGATTGTTTC GGTCTGCCCT 
CCAATGACTC ACCTATGTGA AAAGATCATC TCCACTTTGC CCTCCTGGAG GAAGCTCAAT 
GGCCCCAACC AGCTGATGTC ACTGCAGCAG TTCGTGTACG ACGTACAGGA CCAGTTGAAC 
CCCCTGGCCA GCGAGGCAGA CCTCCGGCAC ATCGCCCAGC AGCTCCACAG TGCTGGCGAG 
ATCAACATCA TGCAGAGCGA GACCGTCCAG GACGTGCTGC TCCTGGATCC CCGCTGGCTG 
TGCACCAACG TTCTGGGGAA GCTGCTCTCT GTGGAGACCC CACGGGCCCT GCACCATTAC 
CGCGGCCGCT ACACCATGGA GGACGTCCAG CGCCTGGTGC CCGACAGTGA CGTGGAGGAG 
CTGCTGCAGA TCCTGGATGC CATGGACATC TGTGCACGGG ACCTGAGCAG CGGGACCATG 
GTGGACATCC CCGCCCTGAT CAAGACTGAC AGCCTACACC GCTCCTGGAC GGAGGAGGAG 
GACGAGGCGC GGGTCTACGG CGGCGTGCGA GTCGTCCCGG TGGAGCACCT CACCCCCTTC 
CCATGTGGCA TCTTCCACAA GGTGCAGGTG AACCTGTGCC GGTGGATCCA CCAGCAGAGC 
AACGAGGGGG ATGCAGACAT TCGCCTGTGG GTGAACGGCT GCAAGATCGC CAATCGCGGG 
GCCGAGCTGC TGGTGCTCCT GGTCAACCAC GGCCAGGGTA TTGAGGTCCA GGTGCGTGGA 
CTGGAGACGG AGAAGATCAA GTGCTGCTTC CTGCTGGACT CCGTGTGCAG CACCATCGAG 
AACGTCATGG CCACCACGCT GCCCGGGCTG CTGACGGTGA AGCACTACCT GAGCCCCCAG 
CAGCTACGAG AGCACCACGA GCCCGTCATG ATCTACCAGC CACGGGACTT CTTCCGGGCG 
CAGACTCTCA AGGAGACCTC ACTGACCAAC ACCATGGGCG GGTACAAGGA GAGCTTTAGC 
AGTATCATGT GCTTCGGCTG TCACGACGTC TACTCGCAGG CCAGCCTCGG CATGGACATC 
CACGCGTCAG ATCTGAACCT CCTGACCCGG AGGAAACTCA GTCGCCTGCT GGACCCGCCC 
GACCCCATGG GGAAGGACTG GTGCCTCCTT GCCATGAACT TGGGCCTCCC CGACCTTGTG 
GCCAAGTACA ACACCAATAA CGGGTCTCCC AAGGAGTTCC TCCCGAGCCC AGTCCACGCC 
CTGCTCCGGG AATGGACTTC CTACCCCGAG AGCACTGTCG GCATTCTCAT GTCCAAACTG 
CGGGAGCTGG GGCGTCGGGA CGCAGCGGAC TTCCTGCTGA AGGCGTCCTC TGTGTTCAAA 
ATCAACCTCG ACGGCAACGG CCAGGAGGCC TACGCCTCAA GCTGCAACAG TGGCACATCT 
TACAATTCCA TTAGCTCGGT CGTGTCCCGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DAPK1 ( XM_024995908.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024851676.1 Bos taurus death associated protein kinase 1 (DAPK1), transcript variant X2, mRNA.

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CloneID	OBa237231
Clone ID Related Accession (Same CDS sequence)	NM_001206038.2 , XM_024995907.1 , XM_024995909.1 , XM_024995910.1 , XM_024995908.1
Accession Version	XM_024995908.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4293bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	death-associated protein kinase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGTGT TCAGGCAGGA AAACGTGGAT GATTACTACG ACACCGGCGA GGAACTTGGC 
AGTGGGCAGT TTGCCGTCGT GAAGAAATGC CGTGAGAAAA GCACCGGTCT ACAGTATGCC 
GCCAAATTCA TCAAGAAAAG GAGGACCAAA TCCAGCCGGC GGGGCGTGAG CCGCGAGGAC 
ATCGAGCGGG AGGTCAGCAT CCTGAAGGAG ATCCAGCACC CCAACGTCAT CACCCTGCAC 
GAGGTTTACG AGAACAAGAC GGACGTCATC CTCATCCTGG AACTCGTTGC AGGTGGTGAG 
CTGTTTGACT TCTTAGCTGA AAAGGAATCT TTGACTGAAG AGGAAGCAAC TGAATTTCTC 
AAACAAATTC TTAATGGTGT TTACTATCTG CACTCCCTTC AGATTGCCCA CTTTGATCTT 
AAGCCCGAAA ACATAATGCT TTTGGATAGA AATGTTCCCA AGCCTCGGAT CAAGATCATT 
GACTTTGGGT TGGCCCATAA AATTGACTTT GGAAATGAAT TCAAAAATAT ATTTGGGACA 
CCAGAATTTG TTGCTCCTGA GATAGTCAAC TATGAACCTC TTGGTCTTGA GGCAGATATG 
TGGAGTATTG GGGTAATAAC CTATATTCTC CTCAGTGGGG CCTCCCCGTT TCTCGGAGAC 
AGTAAGCAAG AAACGTTAGC GAACGTATCT GCCGTCAACT ATGAATTTGA GGAAGAGTAC 
TTCAGTAATA CGAGCGCCCT AGCCAAAGAT TTCATAAGAA GACTCCTCGT CAAGGATCCA 
AAGAAGAGAA TGACAATTCA AGATAGTTTG CAGCATCCCT GGATCAAGCC TAAAGATACC 
CAACAAGCAC TTAGTAGAAA AGCATCAGCA GTAAACATGG AGAAATTCAA GAAGTTTGCA 
GCCCGGAAAA AATGGAAACA ATCTGTTCGC TTGATATCAC TGTGCCAAAG ATTATCCAGG 
TCATTCTTGT CCAGAAGTAA CATGAGTGTT GCCAGAAGCG ATGATACTCT GGATGAGGAA 
GACTCCTTTG TGATGAAAGC TATCATCCAT GCCATCAACG ATGACAATGT CCCAGGCCTG 
CAGCACCTCC TGGGCTCCTT GTCCAACTAC GACGTTAACC AGCCCAACAA GCATGGGACT 
CCTCCATTAC TTATTGCTGC TGGCTGTGGG AATATTCAAA TACTACAGTT GCTCATTAAG 
AGAGGCTCAA GAATTGACAT CCAGGATAAG GGTGGATCCA ATGCCATCTA CTGGGCCTCT 
CGGCATGGCC ATGTAGATAC CTTGAAATTT CTCAATGAGA ACAAATGCCC TCTGGACGTT 
AAAGACAAGT CTGGAGAGAC AGCCCTTCAT GTGGCAGCTC GCTACGGCCA CGCTGATGTG 
GTTCAGTTAC TGTGCAGCTT CGGCTCGAAT CCTAACTTCC AGGACAAGGA AGAAGAAACC 
CCCCTGCACT GTGCTGCCTG GCACGGCTAT TACTCTGTGG CCAAAGCCCT TTGTGAAGCC 
GGCTGCAATG TGAACATTAA GAACAGAGAA GGCGAGACCC CCCTCCTGAC GGCCTCCGCC 
AGGGGCTACC AAGACATCGT GGAGTGTCTG GCCGAGCACG GAGCCGACCT TAATGCATCC 
GACAAGGATG GACACATTGC TCTACATCTT GCCGTGAGAC GATGTCAGAT GGAGGTCATC 
CAGACTCTCA TCAGCCAGGG CAGTTCCGTC GATTTCCAAG ACAGGCATGG CAACACCCCC 
CTCCATGTGG CCTGCAAAGA TGGCAACGTG CCGATTGTGC TGGCCCTCTG TGAAGCCAAC 
TGCAATTTGG ATCTCTCCAA CAAGTATGGA CGGACGCCTC TGCACCTTGC CGCCAACAAC 
GGGATTCTGG ACGTGGTCCG CTACCTGTGT CTGACGGGCG CCAATGTGGA GGCGCTGACC 
TCGGATGGAA AGACAGCAGA AGATCTTGCG AAATCGGAAC AGCATGAACA CGTAGCAGGC 
CTCCTCACAA GACTCCGAAA GGACACACAC CGAGGACTCT TCATCCAGCA ACTGCGACCC 
ACACAGAACC TGCAGCCAAG AATCAAGCTG AAGCTGTTCG GCCACTCGGG GTCTGGGAAG 
ACCACTCTGG TGGAATCTCT CAAGTGTGGG CTGCTAAGGA GCTTCTTCCG AAGACGCCGG 
CCCCGGCTCT CCTCCACCAA CTCTACCCGG TTCCCACCCT CGCCCCTGGC CTCCAAGCCG 
GCAGTGTCCG TGAGCATCAG CAACCTGTAC CCGGGCTGTG AGAACGTGAG TGTCAGGAGC 
CGCAGCATGA TGTTCGAGCC AGGCCTCACC AAGGGGGTGC TGGAGGTGTT CGGGGCCCCG 
TCCCACCATC CGCACTGCGC CACCGATGAC CAGTCCACGA AGGCAATCGA CATCCAGAAT 
GCCTATTTGA ACGGCATTGG TGACTTCAGT GTGTGGGAGT TCTCTGGAAA TCCTGTCTAC 
TTCTGCTGTT ATGACTATTT TGCTGCAAAC GATCCCACGT CCATCCATGT CATCGTTTTC 
AGTCTAGAAG AACCCTATGA GATTCAGCTG AATCAAGTGA TTTTCTGGCT TAGTTTCCTG 
AAGTCTCTTG TCCCAGTGGA AGAACCGATA GCCTTTGGCG GCAAGCTGAA GAACCCACTC 
CGAGTTGTCC TGGTGGCCAC CCATGCCGAC ATCGTGAACC TTCCTCGTTC AGCTGGAGGC 
GAGTTTGGAT ATGACAAAGA CACCTCGTTG CTGAAAGAGA TCAGGAACAG GTTTGGGAAC 
GATCTTCACA TTTCAAATAA GCTCTTTGTT CTGGATGCCG GGGCTTCTGG GTCTAAGGAC 
ATGAAGGTGC TTCGAAATCA CCTGCAAGAA ATACGGAGCC AGATTGTTTC GGTCTGCCCT 
CCAATGACTC ACCTATGTGA AAAGATCATC TCCACTTTGC CCTCCTGGAG GAAGCTCAAT 
GGCCCCAACC AGCTGATGTC ACTGCAGCAG TTCGTGTACG ACGTACAGGA CCAGTTGAAC 
CCCCTGGCCA GCGAGGCAGA CCTCCGGCAC ATCGCCCAGC AGCTCCACAG TGCTGGCGAG 
ATCAACATCA TGCAGAGCGA GACCGTCCAG GACGTGCTGC TCCTGGATCC CCGCTGGCTG 
TGCACCAACG TTCTGGGGAA GCTGCTCTCT GTGGAGACCC CACGGGCCCT GCACCATTAC 
CGCGGCCGCT ACACCATGGA GGACGTCCAG CGCCTGGTGC CCGACAGTGA CGTGGAGGAG 
CTGCTGCAGA TCCTGGATGC CATGGACATC TGTGCACGGG ACCTGAGCAG CGGGACCATG 
GTGGACATCC CCGCCCTGAT CAAGACTGAC AGCCTACACC GCTCCTGGAC GGAGGAGGAG 
GACGAGGCGC GGGTCTACGG CGGCGTGCGA GTCGTCCCGG TGGAGCACCT CACCCCCTTC 
CCATGTGGCA TCTTCCACAA GGTGCAGGTG AACCTGTGCC GGTGGATCCA CCAGCAGAGC 
AACGAGGGGG ATGCAGACAT TCGCCTGTGG GTGAACGGCT GCAAGATCGC CAATCGCGGG 
GCCGAGCTGC TGGTGCTCCT GGTCAACCAC GGCCAGGGTA TTGAGGTCCA GGTGCGTGGA 
CTGGAGACGG AGAAGATCAA GTGCTGCTTC CTGCTGGACT CCGTGTGCAG CACCATCGAG 
AACGTCATGG CCACCACGCT GCCCGGGCTG CTGACGGTGA AGCACTACCT GAGCCCCCAG 
CAGCTACGAG AGCACCACGA GCCCGTCATG ATCTACCAGC CACGGGACTT CTTCCGGGCG 
CAGACTCTCA AGGAGACCTC ACTGACCAAC ACCATGGGCG GGTACAAGGA GAGCTTTAGC 
AGTATCATGT GCTTCGGCTG TCACGACGTC TACTCGCAGG CCAGCCTCGG CATGGACATC 
CACGCGTCAG ATCTGAACCT CCTGACCCGG AGGAAACTCA GTCGCCTGCT GGACCCGCCC 
GACCCCATGG GGAAGGACTG GTGCCTCCTT GCCATGAACT TGGGCCTCCC CGACCTTGTG 
GCCAAGTACA ACACCAATAA CGGGTCTCCC AAGGAGTTCC TCCCGAGCCC AGTCCACGCC 
CTGCTCCGGG AATGGACTTC CTACCCCGAG AGCACTGTCG GCATTCTCAT GTCCAAACTG 
CGGGAGCTGG GGCGTCGGGA CGCAGCGGAC TTCCTGCTGA AGGCGTCCTC TGTGTTCAAA 
ATCAACCTCG ACGGCAACGG CCAGGAGGCC TACGCCTCAA GCTGCAACAG TGGCACATCT 
TACAATTCCA TTAGCTCGGT CGTGTCCCGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024851676.1
CDS	131..4423
Translation	MTVFRQENVDDYYDTGEELGSGQFAVVKKCREKSTGLQYAAKFIKKRRTKSSRRGVSREDIEREVSILKEIQHPNVITLHEVYENKTDVILILELVAGGELFDFLAEKESLTEEEATEFLKQILNGVYYLHSLQIAHFDLKPENIMLLDRNVPKPRIKIIDFGLAHKIDFGNEFKNIFGTPEFVAPEIVNYEPLGLEADMWSIGVITYILLSGASPFLGDSKQETLANVSAVNYEFEEEYFSNTSALAKDFIRRLLVKDPKKRMTIQDSLQHPWIKPKDTQQALSRKASAVNMEKFKKFAARKKWKQSVRLISLCQRLSRSFLSRSNMSVARSDDTLDEEDSFVMKAIIHAINDDNVPGLQHLLGSLSNYDVNQPNKHGTPPLLIAAGCGNIQILQLLIKRGSRIDIQDKGGSNAIYWASRHGHVDTLKFLNENKCPLDVKDKSGETALHVAARYGHADVVQLLCSFGSNPNFQDKEEETPLHCAAWHGYYSVAKALCEAGCNVNIKNREGETPLLTASARGYQDIVECLAEHGADLNASDKDGHIALHLAVRRCQMEVIQTLISQGSSVDFQDRHGNTPLHVACKDGNVPIVLALCEANCNLDLSNKYGRTPLHLAANNGILDVVRYLCLTGANVEALTSDGKTAEDLAKSEQHEHVAGLLTRLRKDTHRGLFIQQLRPTQNLQPRIKLKLFGHSGSGKTTLVESLKCGLLRSFFRRRRPRLSSTNSTRFPPSPLASKPAVSVSISNLYPGCENVSVRSRSMMFEPGLTKGVLEVFGAPSHHPHCATDDQSTKAIDIQNAYLNGIGDFSVWEFSGNPVYFCCYDYFAANDPTSIHVIVFSLEEPYEIQLNQVIFWLSFLKSLVPVEEPIAFGGKLKNPLRVVLVATHADIVNLPRSAGGEFGYDKDTSLLKEIRNRFGNDLHISNKLFVLDAGASGSKDMKVLRNHLQEIRSQIVSVCPPMTHLCEKIISTLPSWRKLNGPNQLMSLQQFVYDVQDQLNPLASEADLRHIAQQLHSAGEINIMQSETVQDVLLLDPRWLCTNVLGKLLSVETPRALHHYRGRYTMEDVQRLVPDSDVEELLQILDAMDICARDLSSGTMVDIPALIKTDSLHRSWTEEEDEARVYGGVRVVPVEHLTPFPCGIFHKVQVNLCRWIHQQSNEGDADIRLWVNGCKIANRGAELLVLLVNHGQGIEVQVRGLETEKIKCCFLLDSVCSTIENVMATTLPGLLTVKHYLSPQQLREHHEPVMIYQPRDFFRAQTLKETSLTNTMGGYKESFSSIMCFGCHDVYSQASLGMDIHASDLNLLTRRKLSRLLDPPDPMGKDWCLLAMNLGLPDLVAKYNTNNGSPKEFLPSPVHALLREWTSYPESTVGILMSKLRELGRRDAADFLLKASSVFKINLDGNGQEAYASSCNSGTSYNSISSVVSR

Target ORF information:

RefSeq Version	XM_024995908.1
Organism	Bos taurus(cattle)
Definition	Bos taurus death associated protein kinase 1 (DAPK1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024995908.1

ORF Insert Sequence:

ATGACCGTGT TCAGGCAGGA AAACGTGGAT GATTACTACG ACACCGGCGA GGAACTTGGC 
AGTGGGCAGT TTGCCGTCGT GAAGAAATGC CGTGAGAAAA GCACCGGTCT ACAGTATGCC 
GCCAAATTCA TCAAGAAAAG GAGGACCAAA TCCAGCCGGC GGGGCGTGAG CCGCGAGGAC 
ATCGAGCGGG AGGTCAGCAT CCTGAAGGAG ATCCAGCACC CCAACGTCAT CACCCTGCAC 
GAGGTTTACG AGAACAAGAC GGACGTCATC CTCATCCTGG AACTCGTTGC AGGTGGTGAG 
CTGTTTGACT TCTTAGCTGA AAAGGAATCT TTGACTGAAG AGGAAGCAAC TGAATTTCTC 
AAACAAATTC TTAATGGTGT TTACTATCTG CACTCCCTTC AGATTGCCCA CTTTGATCTT 
AAGCCCGAAA ACATAATGCT TTTGGATAGA AATGTTCCCA AGCCTCGGAT CAAGATCATT 
GACTTTGGGT TGGCCCATAA AATTGACTTT GGAAATGAAT TCAAAAATAT ATTTGGGACA 
CCAGAATTTG TTGCTCCTGA GATAGTCAAC TATGAACCTC TTGGTCTTGA GGCAGATATG 
TGGAGTATTG GGGTAATAAC CTATATTCTC CTCAGTGGGG CCTCCCCGTT TCTCGGAGAC 
AGTAAGCAAG AAACGTTAGC GAACGTATCT GCCGTCAACT ATGAATTTGA GGAAGAGTAC 
TTCAGTAATA CGAGCGCCCT AGCCAAAGAT TTCATAAGAA GACTCCTCGT CAAGGATCCA 
AAGAAGAGAA TGACAATTCA AGATAGTTTG CAGCATCCCT GGATCAAGCC TAAAGATACC 
CAACAAGCAC TTAGTAGAAA AGCATCAGCA GTAAACATGG AGAAATTCAA GAAGTTTGCA 
GCCCGGAAAA AATGGAAACA ATCTGTTCGC TTGATATCAC TGTGCCAAAG ATTATCCAGG 
TCATTCTTGT CCAGAAGTAA CATGAGTGTT GCCAGAAGCG ATGATACTCT GGATGAGGAA 
GACTCCTTTG TGATGAAAGC TATCATCCAT GCCATCAACG ATGACAATGT CCCAGGCCTG 
CAGCACCTCC TGGGCTCCTT GTCCAACTAC GACGTTAACC AGCCCAACAA GCATGGGACT 
CCTCCATTAC TTATTGCTGC TGGCTGTGGG AATATTCAAA TACTACAGTT GCTCATTAAG 
AGAGGCTCAA GAATTGACAT CCAGGATAAG GGTGGATCCA ATGCCATCTA CTGGGCCTCT 
CGGCATGGCC ATGTAGATAC CTTGAAATTT CTCAATGAGA ACAAATGCCC TCTGGACGTT 
AAAGACAAGT CTGGAGAGAC AGCCCTTCAT GTGGCAGCTC GCTACGGCCA CGCTGATGTG 
GTTCAGTTAC TGTGCAGCTT CGGCTCGAAT CCTAACTTCC AGGACAAGGA AGAAGAAACC 
CCCCTGCACT GTGCTGCCTG GCACGGCTAT TACTCTGTGG CCAAAGCCCT TTGTGAAGCC 
GGCTGCAATG TGAACATTAA GAACAGAGAA GGCGAGACCC CCCTCCTGAC GGCCTCCGCC 
AGGGGCTACC AAGACATCGT GGAGTGTCTG GCCGAGCACG GAGCCGACCT TAATGCATCC 
GACAAGGATG GACACATTGC TCTACATCTT GCCGTGAGAC GATGTCAGAT GGAGGTCATC 
CAGACTCTCA TCAGCCAGGG CAGTTCCGTC GATTTCCAAG ACAGGCATGG CAACACCCCC 
CTCCATGTGG CCTGCAAAGA TGGCAACGTG CCGATTGTGC TGGCCCTCTG TGAAGCCAAC 
TGCAATTTGG ATCTCTCCAA CAAGTATGGA CGGACGCCTC TGCACCTTGC CGCCAACAAC 
GGGATTCTGG ACGTGGTCCG CTACCTGTGT CTGACGGGCG CCAATGTGGA GGCGCTGACC 
TCGGATGGAA AGACAGCAGA AGATCTTGCG AAATCGGAAC AGCATGAACA CGTAGCAGGC 
CTCCTCACAA GACTCCGAAA GGACACACAC CGAGGACTCT TCATCCAGCA ACTGCGACCC 
ACACAGAACC TGCAGCCAAG AATCAAGCTG AAGCTGTTCG GCCACTCGGG GTCTGGGAAG 
ACCACTCTGG TGGAATCTCT CAAGTGTGGG CTGCTAAGGA GCTTCTTCCG AAGACGCCGG 
CCCCGGCTCT CCTCCACCAA CTCTACCCGG TTCCCACCCT CGCCCCTGGC CTCCAAGCCG 
GCAGTGTCCG TGAGCATCAG CAACCTGTAC CCGGGCTGTG AGAACGTGAG TGTCAGGAGC 
CGCAGCATGA TGTTCGAGCC AGGCCTCACC AAGGGGGTGC TGGAGGTGTT CGGGGCCCCG 
TCCCACCATC CGCACTGCGC CACCGATGAC CAGTCCACGA AGGCAATCGA CATCCAGAAT 
GCCTATTTGA ACGGCATTGG TGACTTCAGT GTGTGGGAGT TCTCTGGAAA TCCTGTCTAC 
TTCTGCTGTT ATGACTATTT TGCTGCAAAC GATCCCACGT CCATCCATGT CATCGTTTTC 
AGTCTAGAAG AACCCTATGA GATTCAGCTG AATCAAGTGA TTTTCTGGCT TAGTTTCCTG 
AAGTCTCTTG TCCCAGTGGA AGAACCGATA GCCTTTGGCG GCAAGCTGAA GAACCCACTC 
CGAGTTGTCC TGGTGGCCAC CCATGCCGAC ATCGTGAACC TTCCTCGTTC AGCTGGAGGC 
GAGTTTGGAT ATGACAAAGA CACCTCGTTG CTGAAAGAGA TCAGGAACAG GTTTGGGAAC 
GATCTTCACA TTTCAAATAA GCTCTTTGTT CTGGATGCCG GGGCTTCTGG GTCTAAGGAC 
ATGAAGGTGC TTCGAAATCA CCTGCAAGAA ATACGGAGCC AGATTGTTTC GGTCTGCCCT 
CCAATGACTC ACCTATGTGA AAAGATCATC TCCACTTTGC CCTCCTGGAG GAAGCTCAAT 
GGCCCCAACC AGCTGATGTC ACTGCAGCAG TTCGTGTACG ACGTACAGGA CCAGTTGAAC 
CCCCTGGCCA GCGAGGCAGA CCTCCGGCAC ATCGCCCAGC AGCTCCACAG TGCTGGCGAG 
ATCAACATCA TGCAGAGCGA GACCGTCCAG GACGTGCTGC TCCTGGATCC CCGCTGGCTG 
TGCACCAACG TTCTGGGGAA GCTGCTCTCT GTGGAGACCC CACGGGCCCT GCACCATTAC 
CGCGGCCGCT ACACCATGGA GGACGTCCAG CGCCTGGTGC CCGACAGTGA CGTGGAGGAG 
CTGCTGCAGA TCCTGGATGC CATGGACATC TGTGCACGGG ACCTGAGCAG CGGGACCATG 
GTGGACATCC CCGCCCTGAT CAAGACTGAC AGCCTACACC GCTCCTGGAC GGAGGAGGAG 
GACGAGGCGC GGGTCTACGG CGGCGTGCGA GTCGTCCCGG TGGAGCACCT CACCCCCTTC 
CCATGTGGCA TCTTCCACAA GGTGCAGGTG AACCTGTGCC GGTGGATCCA CCAGCAGAGC 
AACGAGGGGG ATGCAGACAT TCGCCTGTGG GTGAACGGCT GCAAGATCGC CAATCGCGGG 
GCCGAGCTGC TGGTGCTCCT GGTCAACCAC GGCCAGGGTA TTGAGGTCCA GGTGCGTGGA 
CTGGAGACGG AGAAGATCAA GTGCTGCTTC CTGCTGGACT CCGTGTGCAG CACCATCGAG 
AACGTCATGG CCACCACGCT GCCCGGGCTG CTGACGGTGA AGCACTACCT GAGCCCCCAG 
CAGCTACGAG AGCACCACGA GCCCGTCATG ATCTACCAGC CACGGGACTT CTTCCGGGCG 
CAGACTCTCA AGGAGACCTC ACTGACCAAC ACCATGGGCG GGTACAAGGA GAGCTTTAGC 
AGTATCATGT GCTTCGGCTG TCACGACGTC TACTCGCAGG CCAGCCTCGG CATGGACATC 
CACGCGTCAG ATCTGAACCT CCTGACCCGG AGGAAACTCA GTCGCCTGCT GGACCCGCCC 
GACCCCATGG GGAAGGACTG GTGCCTCCTT GCCATGAACT TGGGCCTCCC CGACCTTGTG 
GCCAAGTACA ACACCAATAA CGGGTCTCCC AAGGAGTTCC TCCCGAGCCC AGTCCACGCC 
CTGCTCCGGG AATGGACTTC CTACCCCGAG AGCACTGTCG GCATTCTCAT GTCCAAACTG 
CGGGAGCTGG GGCGTCGGGA CGCAGCGGAC TTCCTGCTGA AGGCGTCCTC TGTGTTCAAA 
ATCAACCTCG ACGGCAACGG CCAGGAGGCC TACGCCTCAA GCTGCAACAG TGGCACATCT 
TACAATTCCA TTAGCTCGGT CGTGTCCCGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DAPK1 ( NM_001206038.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001192967.1 Bos taurus death-associated protein kinase 1 (DAPK1), mRNA.

Price & Availability↑

CloneID	OBa01719
Clone ID Related Accession (Same CDS sequence)	NM_001206038.1
Accession Version	NM_001206038.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4293bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2011-10-24
Organism	Bos taurus(cattle)
Product	death-associated protein kinase 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAFC03094561.1, AAFC03094562.1, AAFC03094565.1, AAFC03053673.1 and AAFC03124808.1. On Apr 20, 2011 this sequence version replaced XM_613544.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

ATGACCGTGT TCAGGCAGGA AAACGTGGAT GATTACTACG ACACCGGCGA GGAACTTGGC 
AGTGGGCAGT TTGCCGTCGT GAAGAAATGC CGTGAGAAAA GCACCGGTCT ACAGTATGCC 
GCCAAATTCA TCAAGAAAAG GAGGACCAAA TCCAGCCGGC GGGGCGTGAG CCGCGAGGAC 
ATCGAGCGGG AGGTCAGCAT CCTGAAGGAG ATCCAGCACC CCAACGTCAT CACCCTGCAC 
GAGGTTTACG AGAACAAGAC GGACGTCATC CTGATCCTGG AACTCGTTGC AGGTGGTGAG 
CTGTTTGACT TCTTAGCTGA AAAGGAATCT TTGACTGAAG AGGAAGCAAC TGAATTTCTC 
AAACAAATTC TTAATGGTGT TTACTATCTG CACTCCCTTC AGATTGCCCA CTTTGATCTT 
AAGCCCGAAA ACATAATGCT TTTGGATAGA AATGTTCCCA AGCCTCGGAT CAAGATCATT 
GACTTTGGGT TGGCCCATAA AATTGACTTT GGAAATGAAT TCAAAAATAT ATTTGGGACA 
CCAGAATTTG TTGCTCCTGA GATAGTCAAC TATGAACCTC TTGGTCTTGA GGCAGATATG 
TGGAGTATTG GGGTAATAAC CTATATTCTC CTCAGTGGGG CCTCCCCGTT TCTCGGAGAC 
AGTAAGCAAG AAACGTTAGC GAACGTATCT GCCGTCAACT ATGAATTTGA GGAAGAGTAC 
TTCAGTAATA CGAGCGCCCT AGCCAAAGAT TTCATAAGAA GACTCCTCGT CAAGGATCCA 
AAGAAGAGAA TGACAATTCA AGATAGTTTG CAGCATCCCT GGATCAAGCC TAAAGATACC 
CAACAAGCAC TTAGTAGAAA AGCATCAGCA GTAAACATGG AGAAATTCAA GAAGTTCGCA 
GCCCGGAAAA AATGGAAACA ATCTGTTCGC TTGATATCAC TGTGCCAAAG ATTATCCAGG 
TCATTCTTGT CCAGAAGTAA CATGAGTGTT GCCAGAAGCG ATGATACTCT GGATGAGGAA 
GACTCCTTTG TGATGAAAGC TATCATCCAT GCCATCAACG ATGACAATGT CCCAGGCCTG 
CAGCACCTCC TGGGCTCCTT GTCCAACTAC GACGTTAACC AGCCCAACAA GCATGGGACT 
CCTCCATTAC TTATTGCTGC TGGCTGTGGG AATATTCAAA TACTACAGTT GCTCATTAAG 
AGAGGCTCAA GAATTGACAT CCAGGATAAG GGTGGATCCA ATGCCATCTA CTGGGCCTCT 
CGGCATGGCC ATGTAGATAC CTTGAAATTT CTCAATGAGA ACAAATGCCC TCTGGACGTT 
AAAGACAAGT CTGGAGAGAC AGCCCTTCAT GTGGCAGCTC GCTACGGCCA CGCTGATGTG 
GTTCAGTTAC TGTGCAGCTT CGGCTCGAAT CCTAACTTCC AGGACAAGGA AGAAGAAACC 
CCCCTGCACT GTGCTGCCTG GCACGGCTAT TACTCTGTGG CCAAAGCCCT TTGTGAAGCC 
GGCTGCAATG TGAACATTAA GAACAGAGAA GGCGAGACCC CCCTCCTGAC GGCCTCCGCC 
AGGGGCTACC AAGACATCGT GGAGTGTCTG GCCGAGCACG GAGCCGACCT TAATGCATCC 
GACAAGGATG GACACATTGC TCTACATCTT GCCGTGAGAC GATGTCAGAT GGAGGTCATC 
CAGACTCTCA TCAGCCAGGG CAGTTCCGTC GATTTCCAAG ACAGGCATGG CAACACCCCC 
CTCCATGTGG CCTGCAAAGA TGGCAACGTG CCGATTGTGC TGGCCCTCTG TGAAGCCAAC 
TGCAATTTGG ATCTCTCCAA CAAGTATGGA CGGACGCCTC TGCACCTTGC CGCCAACAAC 
GGGATTCTGG ACGTGGTCCG CTACCTGTGT CTGACGGGCG CCAATGTGGA GGCGCTGACC 
TCGGATGGAA AGACAGCAGA AGATCTTGCG AAATCGGAAC AGCATGAACA CGTAGCAGGC 
CTCCTCACAA GACTCCGAAA GGACACACAC CGAGGACTCT TCATCCAGCA ACTGCGACCC 
ACACAGAACC TGCAGCCAAG AATCAAGCTG AAGCTGTTCG GCCACTCGGG GTCTGGGAAG 
ACCACTCTGG TGGAATCTCT CAAGTGTGGG CTGCTAAGGA GCTTCTTCCG AAGACGCCGG 
CCCCGGCTCT CCTCCACCAA CTCTACCCGG TTCCCACCCT CGCCCCTGGC CTCCAAGCCG 
GCAGTGTCCG TGAGCATCAG CAACCTGTAC CCGGGCTGTG AGAACGTGAG CGTCAGGAGC 
CGCAGCATGA TGTTCGAGCC AGGCCTCACC AAGGGGGTGC TGGAGGTGTT CGGGGCCCCG 
TCCCACCATC CGCACTGCGC CACCGATGAC CAGTCCACGA AGGCAATCGA CATCCAGAAT 
GCCTATTTGA ACGGCATTGG TGACTTCAGT GTGTGGGAGT TCTCTGGAAA TCCTGTCTAC 
TTCTGCTGTT ATGACTATTT TGCTGCAAAC GATCCCACGT CCATCCATGT CATCGTTTTC 
AGTCTAGAAG AACCCTATGA GATTCAGCTG AATCAAGTGA TTTTCTGGCT TAGTTTCCTG 
AAGTCTCTTG TCCCAGTGGA AGAACCGATA GCCTTTGGCG GCAAGCTGAA GAACCCACTC 
CGAGTTGTCC TGGTGGCCAC CCATGCCGAC ATCGTGAACC TTCCTCGTTC AGCTGGAGGC 
GAGTTTGGAT ATGACAAAGA CACCTCGTTG CTGAAAGAGA TCAGGAACAG GTTTGGGAAC 
GATCTTCACA TTTCAAATAA GCTCTTTGTT CTGGATGCTG GGGCTTCTGG GTCTAAGGAC 
ATGAAGGTGC TTCGAAATCA CCTGCAAGAA ATACGGAGCC AGATTGTTTC GGTCTGCCCT 
CCAATGACTC ACCTATGTGA AAAGATCATC TCCACTTTGC CCTCCTGGAG GAAGCTCAAT 
GGCCCCAACC AGCTGATGTC ACTGCAGCAG TTCGTGTACG ACGTACAGGA CCAGTTGAAC 
CCCCTGGCCA GCGAGGCAGA CCTCCGGCAC ATCGCCCAGC AGCTCCACAG TGCTGGCGAG 
ATCAACATCA TGCAGAGCGA GACCGTCCAG GACGTGCTGC TCCTGGATCC CCGCTGGCTG 
TGCACCAACG TTCTGGGGAA GCTGCTTTCT GTGGAGACCC CACGGGCCCT GCACCATTAC 
CGCGGCCGCT ACACCATGGA GGACGTCCAG CGCCTGGTGC CCGACAGTGA CGTGGAGGAG 
CTGCTGCAGA TCCTGGATGC CATGGACATC TGTGCACGGG ACCTGAGCAG CGGGACCATG 
GTGGACATCC CCGCCCTGAT CAAGACTGAC AGCCTACACC GCTCCTGGAC GGAGGAGGAG 
GACGAGGCGC GGGTCTACGG CGGCGTGCGA GTCGTCCCGG TGGAGCACCT CACCCCCTTC 
CCATGTGGCA TCTTCCACAA GGTGCAGGTG AACCTGTGCC GGTGGATCCA CCAGCAGAGC 
AACGAGGGGG ATGCAGACAT TCGCCTGTGG GTGAACGGCT GCAAGATCGC CAATCGCGGG 
GCCGAGCTGC TGGTGCTCCT GGTCAACCAC GGCCAGGGTA TTGAGGTCCA GGTGCGTGGA 
CTGGAGACGG AGAAGATCAA GTGCTGCTTC CTGCTGGACT CCGTGTGCAG CACCATCGAG 
AACGTCATGG CCACCACGCT GCCCGGGCTG CTGACGGTGA AGCACTACCT GAGCCCCCAG 
CAGCTACGAG AGCACCACGA GCCCGTCATG ATCTACCAGC CACGGGACTT CTTCCGGGCG 
CAGACTCTCA AGGAGACCTC ACTGACCAAC ACCATGGGCG GGTACAAGGA GAGCTTTAGC 
AGTATCATGT GCTTCGGCTG TCACGACGTC TACTCGCAGG CCAGCCTCGG CATGGACATC 
CACGCGTCAG ATCTGAACCT CCTGACCCGG AGGAAACTCA GTCGCCTGCT GGACCCGCCC 
GACCCCATGG GGAAGGACTG GTGCCTCCTT GCCATGAACT TGGGCCTCCC CGACCTTGTG 
GCCAAGTACA ACACCAATAA CGGGTCTCCC AAGGAGTTCC TCCCGAGCCC AGTCCACGCC 
CTGCTCCGGG AATGGACTTC CTACCCCGAG AGCACTGTCG GCATTCTCAT GTCCAAACTG 
CGGGAGCTGG GGCGTCGGGA CGCAGCGGAC TTCCTGCTGA AGGCGTCCTC TGTGTTCAAA 
ATCAACCTCG ACGGCAACGG CCAGGAGGCC TACGCCTCAA GCTGCAACAG TGGCACATCT 
TACAATTCCA TTAGCTCGGT CGTGTCCCGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001192967.1
CDS	296..4588
Translation	MTVFRQENVDDYYDTGEELGSGQFAVVKKCREKSTGLQYAAKFIKKRRTKSSRRGVSREDIEREVSILKEIQHPNVITLHEVYENKTDVILILELVAGGELFDFLAEKESLTEEEATEFLKQILNGVYYLHSLQIAHFDLKPENIMLLDRNVPKPRIKIIDFGLAHKIDFGNEFKNIFGTPEFVAPEIVNYEPLGLEADMWSIGVITYILLSGASPFLGDSKQETLANVSAVNYEFEEEYFSNTSALAKDFIRRLLVKDPKKRMTIQDSLQHPWIKPKDTQQALSRKASAVNMEKFKKFAARKKWKQSVRLISLCQRLSRSFLSRSNMSVARSDDTLDEEDSFVMKAIIHAINDDNVPGLQHLLGSLSNYDVNQPNKHGTPPLLIAAGCGNIQILQLLIKRGSRIDIQDKGGSNAIYWASRHGHVDTLKFLNENKCPLDVKDKSGETALHVAARYGHADVVQLLCSFGSNPNFQDKEEETPLHCAAWHGYYSVAKALCEAGCNVNIKNREGETPLLTASARGYQDIVECLAEHGADLNASDKDGHIALHLAVRRCQMEVIQTLISQGSSVDFQDRHGNTPLHVACKDGNVPIVLALCEANCNLDLSNKYGRTPLHLAANNGILDVVRYLCLTGANVEALTSDGKTAEDLAKSEQHEHVAGLLTRLRKDTHRGLFIQQLRPTQNLQPRIKLKLFGHSGSGKTTLVESLKCGLLRSFFRRRRPRLSSTNSTRFPPSPLASKPAVSVSISNLYPGCENVSVRSRSMMFEPGLTKGVLEVFGAPSHHPHCATDDQSTKAIDIQNAYLNGIGDFSVWEFSGNPVYFCCYDYFAANDPTSIHVIVFSLEEPYEIQLNQVIFWLSFLKSLVPVEEPIAFGGKLKNPLRVVLVATHADIVNLPRSAGGEFGYDKDTSLLKEIRNRFGNDLHISNKLFVLDAGASGSKDMKVLRNHLQEIRSQIVSVCPPMTHLCEKIISTLPSWRKLNGPNQLMSLQQFVYDVQDQLNPLASEADLRHIAQQLHSAGEINIMQSETVQDVLLLDPRWLCTNVLGKLLSVETPRALHHYRGRYTMEDVQRLVPDSDVEELLQILDAMDICARDLSSGTMVDIPALIKTDSLHRSWTEEEDEARVYGGVRVVPVEHLTPFPCGIFHKVQVNLCRWIHQQSNEGDADIRLWVNGCKIANRGAELLVLLVNHGQGIEVQVRGLETEKIKCCFLLDSVCSTIENVMATTLPGLLTVKHYLSPQQLREHHEPVMIYQPRDFFRAQTLKETSLTNTMGGYKESFSSIMCFGCHDVYSQASLGMDIHASDLNLLTRRKLSRLLDPPDPMGKDWCLLAMNLGLPDLVAKYNTNNGSPKEFLPSPVHALLREWTSYPESTVGILMSKLRELGRRDAADFLLKASSVFKINLDGNGQEAYASSCNSGTSYNSISSVVSR

Target ORF information:

RefSeq Version	NM_001206038.1
Organism	Bos taurus(cattle)
Definition	Bos taurus death-associated protein kinase 1 (DAPK1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001206038.1

ORF Insert Sequence:

ATGACCGTGT TCAGGCAGGA AAACGTGGAT GATTACTACG ACACCGGCGA GGAACTTGGC 
AGTGGGCAGT TTGCCGTCGT GAAGAAATGC CGTGAGAAAA GCACCGGTCT ACAGTATGCC 
GCCAAATTCA TCAAGAAAAG GAGGACCAAA TCCAGCCGGC GGGGCGTGAG CCGCGAGGAC 
ATCGAGCGGG AGGTCAGCAT CCTGAAGGAG ATCCAGCACC CCAACGTCAT CACCCTGCAC 
GAGGTTTACG AGAACAAGAC GGACGTCATC CTGATCCTGG AACTCGTTGC AGGTGGTGAG 
CTGTTTGACT TCTTAGCTGA AAAGGAATCT TTGACTGAAG AGGAAGCAAC TGAATTTCTC 
AAACAAATTC TTAATGGTGT TTACTATCTG CACTCCCTTC AGATTGCCCA CTTTGATCTT 
AAGCCCGAAA ACATAATGCT TTTGGATAGA AATGTTCCCA AGCCTCGGAT CAAGATCATT 
GACTTTGGGT TGGCCCATAA AATTGACTTT GGAAATGAAT TCAAAAATAT ATTTGGGACA 
CCAGAATTTG TTGCTCCTGA GATAGTCAAC TATGAACCTC TTGGTCTTGA GGCAGATATG 
TGGAGTATTG GGGTAATAAC CTATATTCTC CTCAGTGGGG CCTCCCCGTT TCTCGGAGAC 
AGTAAGCAAG AAACGTTAGC GAACGTATCT GCCGTCAACT ATGAATTTGA GGAAGAGTAC 
TTCAGTAATA CGAGCGCCCT AGCCAAAGAT TTCATAAGAA GACTCCTCGT CAAGGATCCA 
AAGAAGAGAA TGACAATTCA AGATAGTTTG CAGCATCCCT GGATCAAGCC TAAAGATACC 
CAACAAGCAC TTAGTAGAAA AGCATCAGCA GTAAACATGG AGAAATTCAA GAAGTTCGCA 
GCCCGGAAAA AATGGAAACA ATCTGTTCGC TTGATATCAC TGTGCCAAAG ATTATCCAGG 
TCATTCTTGT CCAGAAGTAA CATGAGTGTT GCCAGAAGCG ATGATACTCT GGATGAGGAA 
GACTCCTTTG TGATGAAAGC TATCATCCAT GCCATCAACG ATGACAATGT CCCAGGCCTG 
CAGCACCTCC TGGGCTCCTT GTCCAACTAC GACGTTAACC AGCCCAACAA GCATGGGACT 
CCTCCATTAC TTATTGCTGC TGGCTGTGGG AATATTCAAA TACTACAGTT GCTCATTAAG 
AGAGGCTCAA GAATTGACAT CCAGGATAAG GGTGGATCCA ATGCCATCTA CTGGGCCTCT 
CGGCATGGCC ATGTAGATAC CTTGAAATTT CTCAATGAGA ACAAATGCCC TCTGGACGTT 
AAAGACAAGT CTGGAGAGAC AGCCCTTCAT GTGGCAGCTC GCTACGGCCA CGCTGATGTG 
GTTCAGTTAC TGTGCAGCTT CGGCTCGAAT CCTAACTTCC AGGACAAGGA AGAAGAAACC 
CCCCTGCACT GTGCTGCCTG GCACGGCTAT TACTCTGTGG CCAAAGCCCT TTGTGAAGCC 
GGCTGCAATG TGAACATTAA GAACAGAGAA GGCGAGACCC CCCTCCTGAC GGCCTCCGCC 
AGGGGCTACC AAGACATCGT GGAGTGTCTG GCCGAGCACG GAGCCGACCT TAATGCATCC 
GACAAGGATG GACACATTGC TCTACATCTT GCCGTGAGAC GATGTCAGAT GGAGGTCATC 
CAGACTCTCA TCAGCCAGGG CAGTTCCGTC GATTTCCAAG ACAGGCATGG CAACACCCCC 
CTCCATGTGG CCTGCAAAGA TGGCAACGTG CCGATTGTGC TGGCCCTCTG TGAAGCCAAC 
TGCAATTTGG ATCTCTCCAA CAAGTATGGA CGGACGCCTC TGCACCTTGC CGCCAACAAC 
GGGATTCTGG ACGTGGTCCG CTACCTGTGT CTGACGGGCG CCAATGTGGA GGCGCTGACC 
TCGGATGGAA AGACAGCAGA AGATCTTGCG AAATCGGAAC AGCATGAACA CGTAGCAGGC 
CTCCTCACAA GACTCCGAAA GGACACACAC CGAGGACTCT TCATCCAGCA ACTGCGACCC 
ACACAGAACC TGCAGCCAAG AATCAAGCTG AAGCTGTTCG GCCACTCGGG GTCTGGGAAG 
ACCACTCTGG TGGAATCTCT CAAGTGTGGG CTGCTAAGGA GCTTCTTCCG AAGACGCCGG 
CCCCGGCTCT CCTCCACCAA CTCTACCCGG TTCCCACCCT CGCCCCTGGC CTCCAAGCCG 
GCAGTGTCCG TGAGCATCAG CAACCTGTAC CCGGGCTGTG AGAACGTGAG CGTCAGGAGC 
CGCAGCATGA TGTTCGAGCC AGGCCTCACC AAGGGGGTGC TGGAGGTGTT CGGGGCCCCG 
TCCCACCATC CGCACTGCGC CACCGATGAC CAGTCCACGA AGGCAATCGA CATCCAGAAT 
GCCTATTTGA ACGGCATTGG TGACTTCAGT GTGTGGGAGT TCTCTGGAAA TCCTGTCTAC 
TTCTGCTGTT ATGACTATTT TGCTGCAAAC GATCCCACGT CCATCCATGT CATCGTTTTC 
AGTCTAGAAG AACCCTATGA GATTCAGCTG AATCAAGTGA TTTTCTGGCT TAGTTTCCTG 
AAGTCTCTTG TCCCAGTGGA AGAACCGATA GCCTTTGGCG GCAAGCTGAA GAACCCACTC 
CGAGTTGTCC TGGTGGCCAC CCATGCCGAC ATCGTGAACC TTCCTCGTTC AGCTGGAGGC 
GAGTTTGGAT ATGACAAAGA CACCTCGTTG CTGAAAGAGA TCAGGAACAG GTTTGGGAAC 
GATCTTCACA TTTCAAATAA GCTCTTTGTT CTGGATGCTG GGGCTTCTGG GTCTAAGGAC 
ATGAAGGTGC TTCGAAATCA CCTGCAAGAA ATACGGAGCC AGATTGTTTC GGTCTGCCCT 
CCAATGACTC ACCTATGTGA AAAGATCATC TCCACTTTGC CCTCCTGGAG GAAGCTCAAT 
GGCCCCAACC AGCTGATGTC ACTGCAGCAG TTCGTGTACG ACGTACAGGA CCAGTTGAAC 
CCCCTGGCCA GCGAGGCAGA CCTCCGGCAC ATCGCCCAGC AGCTCCACAG TGCTGGCGAG 
ATCAACATCA TGCAGAGCGA GACCGTCCAG GACGTGCTGC TCCTGGATCC CCGCTGGCTG 
TGCACCAACG TTCTGGGGAA GCTGCTTTCT GTGGAGACCC CACGGGCCCT GCACCATTAC 
CGCGGCCGCT ACACCATGGA GGACGTCCAG CGCCTGGTGC CCGACAGTGA CGTGGAGGAG 
CTGCTGCAGA TCCTGGATGC CATGGACATC TGTGCACGGG ACCTGAGCAG CGGGACCATG 
GTGGACATCC CCGCCCTGAT CAAGACTGAC AGCCTACACC GCTCCTGGAC GGAGGAGGAG 
GACGAGGCGC GGGTCTACGG CGGCGTGCGA GTCGTCCCGG TGGAGCACCT CACCCCCTTC 
CCATGTGGCA TCTTCCACAA GGTGCAGGTG AACCTGTGCC GGTGGATCCA CCAGCAGAGC 
AACGAGGGGG ATGCAGACAT TCGCCTGTGG GTGAACGGCT GCAAGATCGC CAATCGCGGG 
GCCGAGCTGC TGGTGCTCCT GGTCAACCAC GGCCAGGGTA TTGAGGTCCA GGTGCGTGGA 
CTGGAGACGG AGAAGATCAA GTGCTGCTTC CTGCTGGACT CCGTGTGCAG CACCATCGAG 
AACGTCATGG CCACCACGCT GCCCGGGCTG CTGACGGTGA AGCACTACCT GAGCCCCCAG 
CAGCTACGAG AGCACCACGA GCCCGTCATG ATCTACCAGC CACGGGACTT CTTCCGGGCG 
CAGACTCTCA AGGAGACCTC ACTGACCAAC ACCATGGGCG GGTACAAGGA GAGCTTTAGC 
AGTATCATGT GCTTCGGCTG TCACGACGTC TACTCGCAGG CCAGCCTCGG CATGGACATC 
CACGCGTCAG ATCTGAACCT CCTGACCCGG AGGAAACTCA GTCGCCTGCT GGACCCGCCC 
GACCCCATGG GGAAGGACTG GTGCCTCCTT GCCATGAACT TGGGCCTCCC CGACCTTGTG 
GCCAAGTACA ACACCAATAA CGGGTCTCCC AAGGAGTTCC TCCCGAGCCC AGTCCACGCC 
CTGCTCCGGG AATGGACTTC CTACCCCGAG AGCACTGTCG GCATTCTCAT GTCCAAACTG 
CGGGAGCTGG GGCGTCGGGA CGCAGCGGAC TTCCTGCTGA AGGCGTCCTC TGTGTTCAAA 
ATCAACCTCG ACGGCAACGG CCAGGAGGCC TACGCCTCAA GCTGCAACAG TGGCACATCT 
TACAATTCCA TTAGCTCGGT CGTGTCCCGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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DAPK1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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