dapk1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	dapk1
Entrez Gene ID	100144678
Full Name	death associated protein kinase 1
Synonyms	dapk,dapk1-a,dapk1-b
General protein information	Preferred Names death associated protein kinase 1 Names death-associated protein kinase 1
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012955218.3	XP_012810672.2	death-associated protein kinase 1 isoform X1
XM_012955236.3	XP_012810690.2	death-associated protein kinase 1 isoform X1
NM_001123439.1	NP_001116911.1	death-associated protein kinase 1
XM_012955218.2	XP_012810672.1	death-associated protein kinase 1 isoform X1
XM_012955236.2	XP_012810690.1	death-associated protein kinase 1 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	DAPK1	NP_004929.2
Xenopus tropicalis (tropical clawed frog)	dapk1	NP_001116911.1
Rattus norvegicus (Norway rat)	Dapk1	NP_001100805.2
Danio rerio (zebrafish)	dapk1	NP_001093460.1
Pan troglodytes (chimpanzee)	DAPK1	XP_001140200.1
	DAPK1	XP_001084752.1
Bos taurus (cattle)	DAPK1	NP_001192967.1
Gallus gallus (chicken)	DAPK1	NP_001264960.1
Canis lupus familiaris (dog)	DAPK1	NP_001184086.1
Mus musculus (house mouse)	Dapk1	NP_598823.1
Caenorhabditis elegans (roundworm)	dapk-1	NP_490840.2

Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following dapk1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dapk1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa47480	XM_012955236.3 Latest version!	Xenopus tropicalis death associated protein kinase 1 (dapk1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa47480	XM_012955218.3 Latest version!	Xenopus tropicalis death associated protein kinase 1 (dapk1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa02721	NM_001123439.1 Latest version!	Xenopus tropicalis death associated protein kinase 1 (dapk1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OXa31370	XM_012955218.2 Latest version!	Xenopus tropicalis death-associated protein kinase 1 (dapk1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OXa31370	XM_012955236.2 Latest version!	Xenopus tropicalis death-associated protein kinase 1 (dapk1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dapk1 ( XM_012955218.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012810672.2 Xenopus tropicalis death associated protein kinase 1 (dapk1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa47480
Clone ID Related Accession (Same CDS sequence)	XM_012955218.3 , XM_012955236.3
Accession Version	XM_012955218.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4284bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	death-associated protein kinase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012955218.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGTTT TCAGGCAAGA GAATGTGGAG GATTATTATG ATATAGGAGA GGAGCTTGGA 
AGCGGTCAGT TTGCTGTGGT GAAAAAATGC CAAGAAAAGA GCACAGGCGT GCATTATGCT 
GCAAAATTTA TCAAGAAGCG AAGGACCAAA TCAAGTCGGC GTGGGGTGAC CCGGGAGGAT 
ATAGAGAGGG AGGTCAGCAT ACTGAAGGAG ATCCAGCACC CCAATGTGAT CACGTTGCAC 
GAAGTGTACG AGAATAAAGC AGATGTCATT CTCATTCTGG AGCTAGTTGC AGGAGGTGAG 
CTATTTGACT TTCTTGCTGA AAAGGAGTCT CTGACAGAGG AGGAAGCTAC AGAGTTCATC 
AAGCAGATCC TTAATGGTGT GAATTACCTC CACTCCCTGC ACATTGCCCA CTTTGACCTC 
AAGCCTGAAA ACATCATGCT GCTTGATAGA AACGCACCAA ATCCTCGTAT TAAGATCATA 
GATTTTGGCT TGGCTCACAG AATCGACTCT GGAAATGAAT TTAAGAACAT ATTCGGAACT 
CCAGAATTTG TTGCGCCTGA AATTGTAAAT TATGAACCGC TTGGTCTTGA ATCTGATATG 
TGGAGTATTG GAGTCATAAC ATATATTCTA TTGAGCGGGG CATCTCCATT CCTTGGGGAA 
ACAAAGCAGG AGACCTTGGC TAATATATCT GCTGTCAGTT ATGACTTTGA GGAGGAGTTC 
TTTAGTAGTA CAAGTGACCT GGCCAAAGAT TTTATAAGGC GGCTGCTAGT CAAGGACCCA 
AAAAAAAGGA TGACCATTCA AGACAGTTTG TACCACCCTT GGATTAAGCC AAAAGATACT 
CAGCAAGCGC TGAGCAGGAA AGCCTCTGCA GTCAATATGG AGAAGTTTAA GAAGTTTGCT 
GCTCGCAGAA AATGGAAACA ATCTGTCCGT TTGATATCCC TGTGCCAAAG ACTGTCAAGA 
TCCTTCTTGT CCAGGAGCAA CATGAGTATT GCTAGGAGTG ATGACACACT GGATGAGGAG 
GACTCTTTTG TAATGAAAGC CATCATTCAT GCCATAAATG ACGATAACGT TCCTGGTTTG 
CAGCATCTTT TTGGGTCTTT GTCAAACTAT GATGTGAACC AGCCAAACAA GCATGGCACC 
CCTCCCTTGT TAATTGCTGC TGGCTGTGGG AATATTCAGA TCCTTGAACT GCTCATAAAG 
CGTGGCGCAC AGATTGATGT ACAAGATAAG GCTGGGTCCA ATGCAATTTA CTGGGCTTCT 
CGTCATGGTC ACGTTGAGAC TCTGAAATTT CTACATCAGA ATAACTGTCC TTTAGATATT 
AAAGATAAGT CAGGAGAAAC AGCACTTCAC GTGGCGGCCC GTTATGGACA TGCTGAAGTT 
ATTCAATACT TGTGCAGCAT TGGATCACAT CCAGACATCC AGGATAAGGA GGAAGAAACC 
CCTCTGCATT GTGCTGCTTG GCATGGTTAC TACCCAGTGG CAAAAGCTCT GTGCCAGGCT 
GGCTGCAATG TCAACATTCG CAACAGGGAA GGGGAAACAC CCATTCTGAC AGCAGCAGCA 
AGAGGGTACC ACGATATTGT GGAATGCTTG GTTGACCATG GAGGCGACCT TGATGCAACA 
GACAAGGATG GGCACATAGC ACTCCACCTT GCAGTGAGAC GGTGTCAGAT GGAAGTAGTG 
AAAACGCTCA TCATCCATGG ATGCTTTGTA GACTTCCAGG ATCGCCATGG TAACACACCT 
CTGCATGTTG CCTGCAAAGA TGGGAATCTT CCTATTGTAA TGGCACTGTG TGAGGCAAGA 
TGTAATTTGG ATATAACTAA CAAGTATGGC CGAACACCTT TACATCTGGC AGCAAACAAT 
GGTATAGTGG AAGTTGTCCG ATACCTCTGC GTCGCTGGTG CAAATGTTGA AGCCTTGACT 
TTGGATGGGA AAACAGCAGA GGATCTCGCT AGGTCAGAAC AGCATGAGTC CGTTGTCAGT 
CTGCTCGCAA GGCTTCGAAG GGATACCTAC AGAACTTTGT TCATTCAGCA GCTCAGACCT 
TCACCAACCC CCCAGCCAAG AATTAAACTG AAGTTATTTG GACACATGGG ATCAGGAAAG 
ACCACGCTTG TAGAGTCCCT CAAATGTGGC CTTATCCGAA GCTTCTTCAG AAGACGCAGA 
CCTCGTCTTT CATCAACTAA CTCTTCCCGT TTTCCTTCTT CTCCACTAAC AGCTAAGACA 
GTTTCAGTGA GCATCTCGGA TCTCTACCCT GGTTGTGAGA ATGTTAGTGT AAGAAGTCGG 
AGTATGATGT TTGAGCCAGG ACTAACCAAA GGGGTTCTTG AAGTATTTGT TTCACCGTCC 
CCTCACTGTT ACACAGATGA CCAGTCAACC AAGGCTATAG ACATCCAGAG TGCCAATCTT 
AACGGTGTTG GAGATTTTAG TGTGTGGGAA TTCTCTGGAA ACCCAACGTA TTATTGCAGC 
TATGATTATT TTGCTGCCAA TGATCCAACA TCAGTTCACA TTGTAGTGTT TAGCCTTGAA 
GAACCCTATG AGATTCAGCT GAATCAAGTG ATATTCTGGC TCAATTTCCT AAAGTCACTT 
GTTCCTGTGG AAGAACCAAT AGCGTATGGT GGGAAGCTGA AGAATCTACT CCACGTTGTG 
TTGGTGGCCA CACATGCTGA TATTGTTAAC CTTCCTCGCC CTGCCGGAGG AGAATTTGGT 
TACGACAAAA GTCTAGCATT GCTGAAAGAA GTAAGAAATA GATTTGGAAA CGATCTCCAG 
ATTTTGGATA GACTGTTTGT TCTGGATGCC GGGGGATCAG GGTCCAAGGA GATGAAGCTT 
CTGAGGAATC ACCTCCAGGA ATTAAGAAGC CAGCTGATAT CTGACTGTCC ACCTATGACA 
CCGCTTTGTG AGAAGGTGAT GTCAACTCTT CCATCCTGGA GAAAACAGAA TGGTCCCAAT 
CAACTTCTTT CTCTTCAACA ATTTGTATAT GATGTCCAGG ACCAACTAAA TCCACTAGCA 
AGTGAAGAGG ATGTTAGAAA CCTTGCATTG CAACTGCACA GTATTGGAGA AGTTAATATA 
ATGCAGAGTG AGACCGTCCA AGATGTTGTG CTTCTAGATC CACGATGGCT GTGTCACAAT 
GTCCTCGGAA AGCTACTCTC TGTAGAAAAC CCAAAAGCCC TACACCATTA CAGGGGAAGG 
TATACAATGG AAGATATTCA GCGCTTGGTC ACCGACAGTG ATGTTGATGA ACTTGTGCAG 
ATCCTGGATG CAATGGACAT TTGTGCCCGA GATCTAAGCA GCGGAGCTAT GGTGGACATC 
CCTGCCCTCA TTAAAACAGA CAACCTCCAC CGATCATGGG CTGATGAAGA AGAGGACATA 
ATGATTTATG GAGGTGTTAG AATTGTTCCT GTGGAACACC TTACTCCCTT TCCGTGTGGA 
ATTTTTCACA AAGTTCAAGT TAATTTATGC CGATGGGTAC ATCAACAGAG CGCCGACGGT 
GATGCTGATA TACGTTTATG GGTAAACGGT TGTAAAATAG CAAATCGAGG GGCAGAGGTT 
TTGGTGTTGA TGGTCAACCA TGGTCAAGGA ATTGAAGTGC AAGTTAGGGG TCTGGAAACT 
GAGAAAATCA AGTGTTGTCT ACTTCTTGAA TCCATTTGCA GTATCATTGA CAACTTATTT 
GCAACAACGT TGCCTGGTCT TTTAACTGTA AAACATTACT TGAGCCCTCA ACAGCTAAGA 
GAGCATCACG AGCCACTGAT GGTTTACCAA CCTAGAGACT TTTTTCGGGC CCAAATACAA 
AAGGAGACTT CACTGGCTAA CACCATGGCT GGATACAAAG AGAGCTTTAG CAGCATTCTT 
TGTTTTGGGT GCCTGGATGT TTACTCTCAA GGAAACCTAG GCCTGGATCA CCATGTGTCC 
GATATCAATA TTCTTGTTAG AAGAAAGCTC AGCCGACTTT TAGACCCACC AGATCCCATG 
GGAAAGGACT GGTGTCTACT AGCTATGAAT TTGGGCCTAC CTGACTTAGT TGCCAAATAC 
AATACTAACA ATTCTGTACA AAAAGACTTT TTGCCTAGTC CAATGTGTGC TCTTCTGCAG 
GAATGGAGCA ATTCTCCTGA AAGTACTGTA TCGCTCCTAA TGTCTAAACT GAGAGAACTT 
GGGCGCAGGG ATGCAGCAGA TTTTTTACTA AAAGCATCCT CTGTTTTTAA GGTGACTCTT 
GATATCCATG GACAAGAAGC CTATGCGGCA AGCTGCAACA GCGGCACATC CTATAATTCA 
ATTAGCTCAG TCGTGTCACG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012810672.2
CDS	465..4748
Translation	MTVFRQENVEDYYDIGEELGSGQFAVVKKCQEKSTGVHYAAKFIKKRRTKSSRRGVTREDIEREVSILKEIQHPNVITLHEVYENKADVILILELVAGGELFDFLAEKESLTEEEATEFIKQILNGVNYLHSLHIAHFDLKPENIMLLDRNAPNPRIKIIDFGLAHRIDSGNEFKNIFGTPEFVAPEIVNYEPLGLESDMWSIGVITYILLSGASPFLGETKQETLANISAVSYDFEEEFFSSTSDLAKDFIRRLLVKDPKKRMTIQDSLYHPWIKPKDTQQALSRKASAVNMEKFKKFAARRKWKQSVRLISLCQRLSRSFLSRSNMSIARSDDTLDEEDSFVMKAIIHAINDDNVPGLQHLFGSLSNYDVNQPNKHGTPPLLIAAGCGNIQILELLIKRGAQIDVQDKAGSNAIYWASRHGHVETLKFLHQNNCPLDIKDKSGETALHVAARYGHAEVIQYLCSIGSHPDIQDKEEETPLHCAAWHGYYPVAKALCQAGCNVNIRNREGETPILTAAARGYHDIVECLVDHGGDLDATDKDGHIALHLAVRRCQMEVVKTLIIHGCFVDFQDRHGNTPLHVACKDGNLPIVMALCEARCNLDITNKYGRTPLHLAANNGIVEVVRYLCVAGANVEALTLDGKTAEDLARSEQHESVVSLLARLRRDTYRTLFIQQLRPSPTPQPRIKLKLFGHMGSGKTTLVESLKCGLIRSFFRRRRPRLSSTNSSRFPSSPLTAKTVSVSISDLYPGCENVSVRSRSMMFEPGLTKGVLEVFVSPSPHCYTDDQSTKAIDIQSANLNGVGDFSVWEFSGNPTYYCSYDYFAANDPTSVHIVVFSLEEPYEIQLNQVIFWLNFLKSLVPVEEPIAYGGKLKNLLHVVLVATHADIVNLPRPAGGEFGYDKSLALLKEVRNRFGNDLQILDRLFVLDAGGSGSKEMKLLRNHLQELRSQLISDCPPMTPLCEKVMSTLPSWRKQNGPNQLLSLQQFVYDVQDQLNPLASEEDVRNLALQLHSIGEVNIMQSETVQDVVLLDPRWLCHNVLGKLLSVENPKALHHYRGRYTMEDIQRLVTDSDVDELVQILDAMDICARDLSSGAMVDIPALIKTDNLHRSWADEEEDIMIYGGVRIVPVEHLTPFPCGIFHKVQVNLCRWVHQQSADGDADIRLWVNGCKIANRGAEVLVLMVNHGQGIEVQVRGLETEKIKCCLLLESICSIIDNLFATTLPGLLTVKHYLSPQQLREHHEPLMVYQPRDFFRAQIQKETSLANTMAGYKESFSSILCFGCLDVYSQGNLGLDHHVSDINILVRRKLSRLLDPPDPMGKDWCLLAMNLGLPDLVAKYNTNNSVQKDFLPSPMCALLQEWSNSPESTVSLLMSKLRELGRRDAADFLLKASSVFKVTLDIHGQEAYAASCNSGTSYNSISSVVSR

Target ORF information:

RefSeq Version	XM_012955218.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis death associated protein kinase 1 (dapk1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012955218.3

ORF Insert Sequence:

ATGACTGTTT TCAGGCAAGA GAATGTGGAG GATTATTATG ATATAGGAGA GGAGCTTGGA 
AGCGGTCAGT TTGCTGTGGT GAAAAAATGC CAAGAAAAGA GCACAGGCGT GCATTATGCT 
GCAAAATTTA TCAAGAAGCG AAGGACCAAA TCAAGTCGGC GTGGGGTGAC CCGGGAGGAT 
ATAGAGAGGG AGGTCAGCAT ACTGAAGGAG ATCCAGCACC CCAATGTGAT CACGTTGCAC 
GAAGTGTACG AGAATAAAGC AGATGTCATT CTCATTCTGG AGCTAGTTGC AGGAGGTGAG 
CTATTTGACT TTCTTGCTGA AAAGGAGTCT CTGACAGAGG AGGAAGCTAC AGAGTTCATC 
AAGCAGATCC TTAATGGTGT GAATTACCTC CACTCCCTGC ACATTGCCCA CTTTGACCTC 
AAGCCTGAAA ACATCATGCT GCTTGATAGA AACGCACCAA ATCCTCGTAT TAAGATCATA 
GATTTTGGCT TGGCTCACAG AATCGACTCT GGAAATGAAT TTAAGAACAT ATTCGGAACT 
CCAGAATTTG TTGCGCCTGA AATTGTAAAT TATGAACCGC TTGGTCTTGA ATCTGATATG 
TGGAGTATTG GAGTCATAAC ATATATTCTA TTGAGCGGGG CATCTCCATT CCTTGGGGAA 
ACAAAGCAGG AGACCTTGGC TAATATATCT GCTGTCAGTT ATGACTTTGA GGAGGAGTTC 
TTTAGTAGTA CAAGTGACCT GGCCAAAGAT TTTATAAGGC GGCTGCTAGT CAAGGACCCA 
AAAAAAAGGA TGACCATTCA AGACAGTTTG TACCACCCTT GGATTAAGCC AAAAGATACT 
CAGCAAGCGC TGAGCAGGAA AGCCTCTGCA GTCAATATGG AGAAGTTTAA GAAGTTTGCT 
GCTCGCAGAA AATGGAAACA ATCTGTCCGT TTGATATCCC TGTGCCAAAG ACTGTCAAGA 
TCCTTCTTGT CCAGGAGCAA CATGAGTATT GCTAGGAGTG ATGACACACT GGATGAGGAG 
GACTCTTTTG TAATGAAAGC CATCATTCAT GCCATAAATG ACGATAACGT TCCTGGTTTG 
CAGCATCTTT TTGGGTCTTT GTCAAACTAT GATGTGAACC AGCCAAACAA GCATGGCACC 
CCTCCCTTGT TAATTGCTGC TGGCTGTGGG AATATTCAGA TCCTTGAACT GCTCATAAAG 
CGTGGCGCAC AGATTGATGT ACAAGATAAG GCTGGGTCCA ATGCAATTTA CTGGGCTTCT 
CGTCATGGTC ACGTTGAGAC TCTGAAATTT CTACATCAGA ATAACTGTCC TTTAGATATT 
AAAGATAAGT CAGGAGAAAC AGCACTTCAC GTGGCGGCCC GTTATGGACA TGCTGAAGTT 
ATTCAATACT TGTGCAGCAT TGGATCACAT CCAGACATCC AGGATAAGGA GGAAGAAACC 
CCTCTGCATT GTGCTGCTTG GCATGGTTAC TACCCAGTGG CAAAAGCTCT GTGCCAGGCT 
GGCTGCAATG TCAACATTCG CAACAGGGAA GGGGAAACAC CCATTCTGAC AGCAGCAGCA 
AGAGGGTACC ACGATATTGT GGAATGCTTG GTTGACCATG GAGGCGACCT TGATGCAACA 
GACAAGGATG GGCACATAGC ACTCCACCTT GCAGTGAGAC GGTGTCAGAT GGAAGTAGTG 
AAAACGCTCA TCATCCATGG ATGCTTTGTA GACTTCCAGG ATCGCCATGG TAACACACCT 
CTGCATGTTG CCTGCAAAGA TGGGAATCTT CCTATTGTAA TGGCACTGTG TGAGGCAAGA 
TGTAATTTGG ATATAACTAA CAAGTATGGC CGAACACCTT TACATCTGGC AGCAAACAAT 
GGTATAGTGG AAGTTGTCCG ATACCTCTGC GTCGCTGGTG CAAATGTTGA AGCCTTGACT 
TTGGATGGGA AAACAGCAGA GGATCTCGCT AGGTCAGAAC AGCATGAGTC CGTTGTCAGT 
CTGCTCGCAA GGCTTCGAAG GGATACCTAC AGAACTTTGT TCATTCAGCA GCTCAGACCT 
TCACCAACCC CCCAGCCAAG AATTAAACTG AAGTTATTTG GACACATGGG ATCAGGAAAG 
ACCACGCTTG TAGAGTCCCT CAAATGTGGC CTTATCCGAA GCTTCTTCAG AAGACGCAGA 
CCTCGTCTTT CATCAACTAA CTCTTCCCGT TTTCCTTCTT CTCCACTAAC AGCTAAGACA 
GTTTCAGTGA GCATCTCGGA TCTCTACCCT GGTTGTGAGA ATGTTAGTGT AAGAAGTCGG 
AGTATGATGT TTGAGCCAGG ACTAACCAAA GGGGTTCTTG AAGTATTTGT TTCACCGTCC 
CCTCACTGTT ACACAGATGA CCAGTCAACC AAGGCTATAG ACATCCAGAG TGCCAATCTT 
AACGGTGTTG GAGATTTTAG TGTGTGGGAA TTCTCTGGAA ACCCAACGTA TTATTGCAGC 
TATGATTATT TTGCTGCCAA TGATCCAACA TCAGTTCACA TTGTAGTGTT TAGCCTTGAA 
GAACCCTATG AGATTCAGCT GAATCAAGTG ATATTCTGGC TCAATTTCCT AAAGTCACTT 
GTTCCTGTGG AAGAACCAAT AGCGTATGGT GGGAAGCTGA AGAATCTACT CCACGTTGTG 
TTGGTGGCCA CACATGCTGA TATTGTTAAC CTTCCTCGCC CTGCCGGAGG AGAATTTGGT 
TACGACAAAA GTCTAGCATT GCTGAAAGAA GTAAGAAATA GATTTGGAAA CGATCTCCAG 
ATTTTGGATA GACTGTTTGT TCTGGATGCC GGGGGATCAG GGTCCAAGGA GATGAAGCTT 
CTGAGGAATC ACCTCCAGGA ATTAAGAAGC CAGCTGATAT CTGACTGTCC ACCTATGACA 
CCGCTTTGTG AGAAGGTGAT GTCAACTCTT CCATCCTGGA GAAAACAGAA TGGTCCCAAT 
CAACTTCTTT CTCTTCAACA ATTTGTATAT GATGTCCAGG ACCAACTAAA TCCACTAGCA 
AGTGAAGAGG ATGTTAGAAA CCTTGCATTG CAACTGCACA GTATTGGAGA AGTTAATATA 
ATGCAGAGTG AGACCGTCCA AGATGTTGTG CTTCTAGATC CACGATGGCT GTGTCACAAT 
GTCCTCGGAA AGCTACTCTC TGTAGAAAAC CCAAAAGCCC TACACCATTA CAGGGGAAGG 
TATACAATGG AAGATATTCA GCGCTTGGTC ACCGACAGTG ATGTTGATGA ACTTGTGCAG 
ATCCTGGATG CAATGGACAT TTGTGCCCGA GATCTAAGCA GCGGAGCTAT GGTGGACATC 
CCTGCCCTCA TTAAAACAGA CAACCTCCAC CGATCATGGG CTGATGAAGA AGAGGACATA 
ATGATTTATG GAGGTGTTAG AATTGTTCCT GTGGAACACC TTACTCCCTT TCCGTGTGGA 
ATTTTTCACA AAGTTCAAGT TAATTTATGC CGATGGGTAC ATCAACAGAG CGCCGACGGT 
GATGCTGATA TACGTTTATG GGTAAACGGT TGTAAAATAG CAAATCGAGG GGCAGAGGTT 
TTGGTGTTGA TGGTCAACCA TGGTCAAGGA ATTGAAGTGC AAGTTAGGGG TCTGGAAACT 
GAGAAAATCA AGTGTTGTCT ACTTCTTGAA TCCATTTGCA GTATCATTGA CAACTTATTT 
GCAACAACGT TGCCTGGTCT TTTAACTGTA AAACATTACT TGAGCCCTCA ACAGCTAAGA 
GAGCATCACG AGCCACTGAT GGTTTACCAA CCTAGAGACT TTTTTCGGGC CCAAATACAA 
AAGGAGACTT CACTGGCTAA CACCATGGCT GGATACAAAG AGAGCTTTAG CAGCATTCTT 
TGTTTTGGGT GCCTGGATGT TTACTCTCAA GGAAACCTAG GCCTGGATCA CCATGTGTCC 
GATATCAATA TTCTTGTTAG AAGAAAGCTC AGCCGACTTT TAGACCCACC AGATCCCATG 
GGAAAGGACT GGTGTCTACT AGCTATGAAT TTGGGCCTAC CTGACTTAGT TGCCAAATAC 
AATACTAACA ATTCTGTACA AAAAGACTTT TTGCCTAGTC CAATGTGTGC TCTTCTGCAG 
GAATGGAGCA ATTCTCCTGA AAGTACTGTA TCGCTCCTAA TGTCTAAACT GAGAGAACTT 
GGGCGCAGGG ATGCAGCAGA TTTTTTACTA AAAGCATCCT CTGTTTTTAA GGTGACTCTT 
GATATCCATG GACAAGAAGC CTATGCGGCA AGCTGCAACA GCGGCACATC CTATAATTCA 
ATTAGCTCAG TCGTGTCACG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dapk1 ( XM_012955236.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012810690.2 Xenopus tropicalis death associated protein kinase 1 (dapk1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa47480
Clone ID Related Accession (Same CDS sequence)	XM_012955218.3 , XM_012955236.3
Accession Version	XM_012955236.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4284bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	death-associated protein kinase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012955236.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGTTT TCAGGCAAGA GAATGTGGAG GATTATTATG ATATAGGAGA GGAGCTTGGA 
AGCGGTCAGT TTGCTGTGGT GAAAAAATGC CAAGAAAAGA GCACAGGCGT GCATTATGCT 
GCAAAATTTA TCAAGAAGCG AAGGACCAAA TCAAGTCGGC GTGGGGTGAC CCGGGAGGAT 
ATAGAGAGGG AGGTCAGCAT ACTGAAGGAG ATCCAGCACC CCAATGTGAT CACGTTGCAC 
GAAGTGTACG AGAATAAAGC AGATGTCATT CTCATTCTGG AGCTAGTTGC AGGAGGTGAG 
CTATTTGACT TTCTTGCTGA AAAGGAGTCT CTGACAGAGG AGGAAGCTAC AGAGTTCATC 
AAGCAGATCC TTAATGGTGT GAATTACCTC CACTCCCTGC ACATTGCCCA CTTTGACCTC 
AAGCCTGAAA ACATCATGCT GCTTGATAGA AACGCACCAA ATCCTCGTAT TAAGATCATA 
GATTTTGGCT TGGCTCACAG AATCGACTCT GGAAATGAAT TTAAGAACAT ATTCGGAACT 
CCAGAATTTG TTGCGCCTGA AATTGTAAAT TATGAACCGC TTGGTCTTGA ATCTGATATG 
TGGAGTATTG GAGTCATAAC ATATATTCTA TTGAGCGGGG CATCTCCATT CCTTGGGGAA 
ACAAAGCAGG AGACCTTGGC TAATATATCT GCTGTCAGTT ATGACTTTGA GGAGGAGTTC 
TTTAGTAGTA CAAGTGACCT GGCCAAAGAT TTTATAAGGC GGCTGCTAGT CAAGGACCCA 
AAAAAAAGGA TGACCATTCA AGACAGTTTG TACCACCCTT GGATTAAGCC AAAAGATACT 
CAGCAAGCGC TGAGCAGGAA AGCCTCTGCA GTCAATATGG AGAAGTTTAA GAAGTTTGCT 
GCTCGCAGAA AATGGAAACA ATCTGTCCGT TTGATATCCC TGTGCCAAAG ACTGTCAAGA 
TCCTTCTTGT CCAGGAGCAA CATGAGTATT GCTAGGAGTG ATGACACACT GGATGAGGAG 
GACTCTTTTG TAATGAAAGC CATCATTCAT GCCATAAATG ACGATAACGT TCCTGGTTTG 
CAGCATCTTT TTGGGTCTTT GTCAAACTAT GATGTGAACC AGCCAAACAA GCATGGCACC 
CCTCCCTTGT TAATTGCTGC TGGCTGTGGG AATATTCAGA TCCTTGAACT GCTCATAAAG 
CGTGGCGCAC AGATTGATGT ACAAGATAAG GCTGGGTCCA ATGCAATTTA CTGGGCTTCT 
CGTCATGGTC ACGTTGAGAC TCTGAAATTT CTACATCAGA ATAACTGTCC TTTAGATATT 
AAAGATAAGT CAGGAGAAAC AGCACTTCAC GTGGCGGCCC GTTATGGACA TGCTGAAGTT 
ATTCAATACT TGTGCAGCAT TGGATCACAT CCAGACATCC AGGATAAGGA GGAAGAAACC 
CCTCTGCATT GTGCTGCTTG GCATGGTTAC TACCCAGTGG CAAAAGCTCT GTGCCAGGCT 
GGCTGCAATG TCAACATTCG CAACAGGGAA GGGGAAACAC CCATTCTGAC AGCAGCAGCA 
AGAGGGTACC ACGATATTGT GGAATGCTTG GTTGACCATG GAGGCGACCT TGATGCAACA 
GACAAGGATG GGCACATAGC ACTCCACCTT GCAGTGAGAC GGTGTCAGAT GGAAGTAGTG 
AAAACGCTCA TCATCCATGG ATGCTTTGTA GACTTCCAGG ATCGCCATGG TAACACACCT 
CTGCATGTTG CCTGCAAAGA TGGGAATCTT CCTATTGTAA TGGCACTGTG TGAGGCAAGA 
TGTAATTTGG ATATAACTAA CAAGTATGGC CGAACACCTT TACATCTGGC AGCAAACAAT 
GGTATAGTGG AAGTTGTCCG ATACCTCTGC GTCGCTGGTG CAAATGTTGA AGCCTTGACT 
TTGGATGGGA AAACAGCAGA GGATCTCGCT AGGTCAGAAC AGCATGAGTC CGTTGTCAGT 
CTGCTCGCAA GGCTTCGAAG GGATACCTAC AGAACTTTGT TCATTCAGCA GCTCAGACCT 
TCACCAACCC CCCAGCCAAG AATTAAACTG AAGTTATTTG GACACATGGG ATCAGGAAAG 
ACCACGCTTG TAGAGTCCCT CAAATGTGGC CTTATCCGAA GCTTCTTCAG AAGACGCAGA 
CCTCGTCTTT CATCAACTAA CTCTTCCCGT TTTCCTTCTT CTCCACTAAC AGCTAAGACA 
GTTTCAGTGA GCATCTCGGA TCTCTACCCT GGTTGTGAGA ATGTTAGTGT AAGAAGTCGG 
AGTATGATGT TTGAGCCAGG ACTAACCAAA GGGGTTCTTG AAGTATTTGT TTCACCGTCC 
CCTCACTGTT ACACAGATGA CCAGTCAACC AAGGCTATAG ACATCCAGAG TGCCAATCTT 
AACGGTGTTG GAGATTTTAG TGTGTGGGAA TTCTCTGGAA ACCCAACGTA TTATTGCAGC 
TATGATTATT TTGCTGCCAA TGATCCAACA TCAGTTCACA TTGTAGTGTT TAGCCTTGAA 
GAACCCTATG AGATTCAGCT GAATCAAGTG ATATTCTGGC TCAATTTCCT AAAGTCACTT 
GTTCCTGTGG AAGAACCAAT AGCGTATGGT GGGAAGCTGA AGAATCTACT CCACGTTGTG 
TTGGTGGCCA CACATGCTGA TATTGTTAAC CTTCCTCGCC CTGCCGGAGG AGAATTTGGT 
TACGACAAAA GTCTAGCATT GCTGAAAGAA GTAAGAAATA GATTTGGAAA CGATCTCCAG 
ATTTTGGATA GACTGTTTGT TCTGGATGCC GGGGGATCAG GGTCCAAGGA GATGAAGCTT 
CTGAGGAATC ACCTCCAGGA ATTAAGAAGC CAGCTGATAT CTGACTGTCC ACCTATGACA 
CCGCTTTGTG AGAAGGTGAT GTCAACTCTT CCATCCTGGA GAAAACAGAA TGGTCCCAAT 
CAACTTCTTT CTCTTCAACA ATTTGTATAT GATGTCCAGG ACCAACTAAA TCCACTAGCA 
AGTGAAGAGG ATGTTAGAAA CCTTGCATTG CAACTGCACA GTATTGGAGA AGTTAATATA 
ATGCAGAGTG AGACCGTCCA AGATGTTGTG CTTCTAGATC CACGATGGCT GTGTCACAAT 
GTCCTCGGAA AGCTACTCTC TGTAGAAAAC CCAAAAGCCC TACACCATTA CAGGGGAAGG 
TATACAATGG AAGATATTCA GCGCTTGGTC ACCGACAGTG ATGTTGATGA ACTTGTGCAG 
ATCCTGGATG CAATGGACAT TTGTGCCCGA GATCTAAGCA GCGGAGCTAT GGTGGACATC 
CCTGCCCTCA TTAAAACAGA CAACCTCCAC CGATCATGGG CTGATGAAGA AGAGGACATA 
ATGATTTATG GAGGTGTTAG AATTGTTCCT GTGGAACACC TTACTCCCTT TCCGTGTGGA 
ATTTTTCACA AAGTTCAAGT TAATTTATGC CGATGGGTAC ATCAACAGAG CGCCGACGGT 
GATGCTGATA TACGTTTATG GGTAAACGGT TGTAAAATAG CAAATCGAGG GGCAGAGGTT 
TTGGTGTTGA TGGTCAACCA TGGTCAAGGA ATTGAAGTGC AAGTTAGGGG TCTGGAAACT 
GAGAAAATCA AGTGTTGTCT ACTTCTTGAA TCCATTTGCA GTATCATTGA CAACTTATTT 
GCAACAACGT TGCCTGGTCT TTTAACTGTA AAACATTACT TGAGCCCTCA ACAGCTAAGA 
GAGCATCACG AGCCACTGAT GGTTTACCAA CCTAGAGACT TTTTTCGGGC CCAAATACAA 
AAGGAGACTT CACTGGCTAA CACCATGGCT GGATACAAAG AGAGCTTTAG CAGCATTCTT 
TGTTTTGGGT GCCTGGATGT TTACTCTCAA GGAAACCTAG GCCTGGATCA CCATGTGTCC 
GATATCAATA TTCTTGTTAG AAGAAAGCTC AGCCGACTTT TAGACCCACC AGATCCCATG 
GGAAAGGACT GGTGTCTACT AGCTATGAAT TTGGGCCTAC CTGACTTAGT TGCCAAATAC 
AATACTAACA ATTCTGTACA AAAAGACTTT TTGCCTAGTC CAATGTGTGC TCTTCTGCAG 
GAATGGAGCA ATTCTCCTGA AAGTACTGTA TCGCTCCTAA TGTCTAAACT GAGAGAACTT 
GGGCGCAGGG ATGCAGCAGA TTTTTTACTA AAAGCATCCT CTGTTTTTAA GGTGACTCTT 
GATATCCATG GACAAGAAGC CTATGCGGCA AGCTGCAACA GCGGCACATC CTATAATTCA 
ATTAGCTCAG TCGTGTCACG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012810690.2
CDS	197..4480
Translation	MTVFRQENVEDYYDIGEELGSGQFAVVKKCQEKSTGVHYAAKFIKKRRTKSSRRGVTREDIEREVSILKEIQHPNVITLHEVYENKADVILILELVAGGELFDFLAEKESLTEEEATEFIKQILNGVNYLHSLHIAHFDLKPENIMLLDRNAPNPRIKIIDFGLAHRIDSGNEFKNIFGTPEFVAPEIVNYEPLGLESDMWSIGVITYILLSGASPFLGETKQETLANISAVSYDFEEEFFSSTSDLAKDFIRRLLVKDPKKRMTIQDSLYHPWIKPKDTQQALSRKASAVNMEKFKKFAARRKWKQSVRLISLCQRLSRSFLSRSNMSIARSDDTLDEEDSFVMKAIIHAINDDNVPGLQHLFGSLSNYDVNQPNKHGTPPLLIAAGCGNIQILELLIKRGAQIDVQDKAGSNAIYWASRHGHVETLKFLHQNNCPLDIKDKSGETALHVAARYGHAEVIQYLCSIGSHPDIQDKEEETPLHCAAWHGYYPVAKALCQAGCNVNIRNREGETPILTAAARGYHDIVECLVDHGGDLDATDKDGHIALHLAVRRCQMEVVKTLIIHGCFVDFQDRHGNTPLHVACKDGNLPIVMALCEARCNLDITNKYGRTPLHLAANNGIVEVVRYLCVAGANVEALTLDGKTAEDLARSEQHESVVSLLARLRRDTYRTLFIQQLRPSPTPQPRIKLKLFGHMGSGKTTLVESLKCGLIRSFFRRRRPRLSSTNSSRFPSSPLTAKTVSVSISDLYPGCENVSVRSRSMMFEPGLTKGVLEVFVSPSPHCYTDDQSTKAIDIQSANLNGVGDFSVWEFSGNPTYYCSYDYFAANDPTSVHIVVFSLEEPYEIQLNQVIFWLNFLKSLVPVEEPIAYGGKLKNLLHVVLVATHADIVNLPRPAGGEFGYDKSLALLKEVRNRFGNDLQILDRLFVLDAGGSGSKEMKLLRNHLQELRSQLISDCPPMTPLCEKVMSTLPSWRKQNGPNQLLSLQQFVYDVQDQLNPLASEEDVRNLALQLHSIGEVNIMQSETVQDVVLLDPRWLCHNVLGKLLSVENPKALHHYRGRYTMEDIQRLVTDSDVDELVQILDAMDICARDLSSGAMVDIPALIKTDNLHRSWADEEEDIMIYGGVRIVPVEHLTPFPCGIFHKVQVNLCRWVHQQSADGDADIRLWVNGCKIANRGAEVLVLMVNHGQGIEVQVRGLETEKIKCCLLLESICSIIDNLFATTLPGLLTVKHYLSPQQLREHHEPLMVYQPRDFFRAQIQKETSLANTMAGYKESFSSILCFGCLDVYSQGNLGLDHHVSDINILVRRKLSRLLDPPDPMGKDWCLLAMNLGLPDLVAKYNTNNSVQKDFLPSPMCALLQEWSNSPESTVSLLMSKLRELGRRDAADFLLKASSVFKVTLDIHGQEAYAASCNSGTSYNSISSVVSR

Target ORF information:

RefSeq Version	XM_012955236.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis death associated protein kinase 1 (dapk1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012955236.3

ORF Insert Sequence:

ATGACTGTTT TCAGGCAAGA GAATGTGGAG GATTATTATG ATATAGGAGA GGAGCTTGGA 
AGCGGTCAGT TTGCTGTGGT GAAAAAATGC CAAGAAAAGA GCACAGGCGT GCATTATGCT 
GCAAAATTTA TCAAGAAGCG AAGGACCAAA TCAAGTCGGC GTGGGGTGAC CCGGGAGGAT 
ATAGAGAGGG AGGTCAGCAT ACTGAAGGAG ATCCAGCACC CCAATGTGAT CACGTTGCAC 
GAAGTGTACG AGAATAAAGC AGATGTCATT CTCATTCTGG AGCTAGTTGC AGGAGGTGAG 
CTATTTGACT TTCTTGCTGA AAAGGAGTCT CTGACAGAGG AGGAAGCTAC AGAGTTCATC 
AAGCAGATCC TTAATGGTGT GAATTACCTC CACTCCCTGC ACATTGCCCA CTTTGACCTC 
AAGCCTGAAA ACATCATGCT GCTTGATAGA AACGCACCAA ATCCTCGTAT TAAGATCATA 
GATTTTGGCT TGGCTCACAG AATCGACTCT GGAAATGAAT TTAAGAACAT ATTCGGAACT 
CCAGAATTTG TTGCGCCTGA AATTGTAAAT TATGAACCGC TTGGTCTTGA ATCTGATATG 
TGGAGTATTG GAGTCATAAC ATATATTCTA TTGAGCGGGG CATCTCCATT CCTTGGGGAA 
ACAAAGCAGG AGACCTTGGC TAATATATCT GCTGTCAGTT ATGACTTTGA GGAGGAGTTC 
TTTAGTAGTA CAAGTGACCT GGCCAAAGAT TTTATAAGGC GGCTGCTAGT CAAGGACCCA 
AAAAAAAGGA TGACCATTCA AGACAGTTTG TACCACCCTT GGATTAAGCC AAAAGATACT 
CAGCAAGCGC TGAGCAGGAA AGCCTCTGCA GTCAATATGG AGAAGTTTAA GAAGTTTGCT 
GCTCGCAGAA AATGGAAACA ATCTGTCCGT TTGATATCCC TGTGCCAAAG ACTGTCAAGA 
TCCTTCTTGT CCAGGAGCAA CATGAGTATT GCTAGGAGTG ATGACACACT GGATGAGGAG 
GACTCTTTTG TAATGAAAGC CATCATTCAT GCCATAAATG ACGATAACGT TCCTGGTTTG 
CAGCATCTTT TTGGGTCTTT GTCAAACTAT GATGTGAACC AGCCAAACAA GCATGGCACC 
CCTCCCTTGT TAATTGCTGC TGGCTGTGGG AATATTCAGA TCCTTGAACT GCTCATAAAG 
CGTGGCGCAC AGATTGATGT ACAAGATAAG GCTGGGTCCA ATGCAATTTA CTGGGCTTCT 
CGTCATGGTC ACGTTGAGAC TCTGAAATTT CTACATCAGA ATAACTGTCC TTTAGATATT 
AAAGATAAGT CAGGAGAAAC AGCACTTCAC GTGGCGGCCC GTTATGGACA TGCTGAAGTT 
ATTCAATACT TGTGCAGCAT TGGATCACAT CCAGACATCC AGGATAAGGA GGAAGAAACC 
CCTCTGCATT GTGCTGCTTG GCATGGTTAC TACCCAGTGG CAAAAGCTCT GTGCCAGGCT 
GGCTGCAATG TCAACATTCG CAACAGGGAA GGGGAAACAC CCATTCTGAC AGCAGCAGCA 
AGAGGGTACC ACGATATTGT GGAATGCTTG GTTGACCATG GAGGCGACCT TGATGCAACA 
GACAAGGATG GGCACATAGC ACTCCACCTT GCAGTGAGAC GGTGTCAGAT GGAAGTAGTG 
AAAACGCTCA TCATCCATGG ATGCTTTGTA GACTTCCAGG ATCGCCATGG TAACACACCT 
CTGCATGTTG CCTGCAAAGA TGGGAATCTT CCTATTGTAA TGGCACTGTG TGAGGCAAGA 
TGTAATTTGG ATATAACTAA CAAGTATGGC CGAACACCTT TACATCTGGC AGCAAACAAT 
GGTATAGTGG AAGTTGTCCG ATACCTCTGC GTCGCTGGTG CAAATGTTGA AGCCTTGACT 
TTGGATGGGA AAACAGCAGA GGATCTCGCT AGGTCAGAAC AGCATGAGTC CGTTGTCAGT 
CTGCTCGCAA GGCTTCGAAG GGATACCTAC AGAACTTTGT TCATTCAGCA GCTCAGACCT 
TCACCAACCC CCCAGCCAAG AATTAAACTG AAGTTATTTG GACACATGGG ATCAGGAAAG 
ACCACGCTTG TAGAGTCCCT CAAATGTGGC CTTATCCGAA GCTTCTTCAG AAGACGCAGA 
CCTCGTCTTT CATCAACTAA CTCTTCCCGT TTTCCTTCTT CTCCACTAAC AGCTAAGACA 
GTTTCAGTGA GCATCTCGGA TCTCTACCCT GGTTGTGAGA ATGTTAGTGT AAGAAGTCGG 
AGTATGATGT TTGAGCCAGG ACTAACCAAA GGGGTTCTTG AAGTATTTGT TTCACCGTCC 
CCTCACTGTT ACACAGATGA CCAGTCAACC AAGGCTATAG ACATCCAGAG TGCCAATCTT 
AACGGTGTTG GAGATTTTAG TGTGTGGGAA TTCTCTGGAA ACCCAACGTA TTATTGCAGC 
TATGATTATT TTGCTGCCAA TGATCCAACA TCAGTTCACA TTGTAGTGTT TAGCCTTGAA 
GAACCCTATG AGATTCAGCT GAATCAAGTG ATATTCTGGC TCAATTTCCT AAAGTCACTT 
GTTCCTGTGG AAGAACCAAT AGCGTATGGT GGGAAGCTGA AGAATCTACT CCACGTTGTG 
TTGGTGGCCA CACATGCTGA TATTGTTAAC CTTCCTCGCC CTGCCGGAGG AGAATTTGGT 
TACGACAAAA GTCTAGCATT GCTGAAAGAA GTAAGAAATA GATTTGGAAA CGATCTCCAG 
ATTTTGGATA GACTGTTTGT TCTGGATGCC GGGGGATCAG GGTCCAAGGA GATGAAGCTT 
CTGAGGAATC ACCTCCAGGA ATTAAGAAGC CAGCTGATAT CTGACTGTCC ACCTATGACA 
CCGCTTTGTG AGAAGGTGAT GTCAACTCTT CCATCCTGGA GAAAACAGAA TGGTCCCAAT 
CAACTTCTTT CTCTTCAACA ATTTGTATAT GATGTCCAGG ACCAACTAAA TCCACTAGCA 
AGTGAAGAGG ATGTTAGAAA CCTTGCATTG CAACTGCACA GTATTGGAGA AGTTAATATA 
ATGCAGAGTG AGACCGTCCA AGATGTTGTG CTTCTAGATC CACGATGGCT GTGTCACAAT 
GTCCTCGGAA AGCTACTCTC TGTAGAAAAC CCAAAAGCCC TACACCATTA CAGGGGAAGG 
TATACAATGG AAGATATTCA GCGCTTGGTC ACCGACAGTG ATGTTGATGA ACTTGTGCAG 
ATCCTGGATG CAATGGACAT TTGTGCCCGA GATCTAAGCA GCGGAGCTAT GGTGGACATC 
CCTGCCCTCA TTAAAACAGA CAACCTCCAC CGATCATGGG CTGATGAAGA AGAGGACATA 
ATGATTTATG GAGGTGTTAG AATTGTTCCT GTGGAACACC TTACTCCCTT TCCGTGTGGA 
ATTTTTCACA AAGTTCAAGT TAATTTATGC CGATGGGTAC ATCAACAGAG CGCCGACGGT 
GATGCTGATA TACGTTTATG GGTAAACGGT TGTAAAATAG CAAATCGAGG GGCAGAGGTT 
TTGGTGTTGA TGGTCAACCA TGGTCAAGGA ATTGAAGTGC AAGTTAGGGG TCTGGAAACT 
GAGAAAATCA AGTGTTGTCT ACTTCTTGAA TCCATTTGCA GTATCATTGA CAACTTATTT 
GCAACAACGT TGCCTGGTCT TTTAACTGTA AAACATTACT TGAGCCCTCA ACAGCTAAGA 
GAGCATCACG AGCCACTGAT GGTTTACCAA CCTAGAGACT TTTTTCGGGC CCAAATACAA 
AAGGAGACTT CACTGGCTAA CACCATGGCT GGATACAAAG AGAGCTTTAG CAGCATTCTT 
TGTTTTGGGT GCCTGGATGT TTACTCTCAA GGAAACCTAG GCCTGGATCA CCATGTGTCC 
GATATCAATA TTCTTGTTAG AAGAAAGCTC AGCCGACTTT TAGACCCACC AGATCCCATG 
GGAAAGGACT GGTGTCTACT AGCTATGAAT TTGGGCCTAC CTGACTTAGT TGCCAAATAC 
AATACTAACA ATTCTGTACA AAAAGACTTT TTGCCTAGTC CAATGTGTGC TCTTCTGCAG 
GAATGGAGCA ATTCTCCTGA AAGTACTGTA TCGCTCCTAA TGTCTAAACT GAGAGAACTT 
GGGCGCAGGG ATGCAGCAGA TTTTTTACTA AAAGCATCCT CTGTTTTTAA GGTGACTCTT 
GATATCCATG GACAAGAAGC CTATGCGGCA AGCTGCAACA GCGGCACATC CTATAATTCA 
ATTAGCTCAG TCGTGTCACG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dapk1 ( NM_001123439.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001116911.1 Xenopus tropicalis death associated protein kinase 1 (dapk1), mRNA.

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CloneID	OXa02721
Clone ID Related Accession (Same CDS sequence)	NM_001123439.1
Accession Version	NM_001123439.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4284bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Xenopus tropicalis(tropical clawed frog)
Product	death-associated protein kinase 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC159135.1. ##Evidence-Data-START## Transcript exon combination :: BC159135.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00028316, SAMD00028317 [ECO:0000348] ##Evidence-Data-END##

ATGACTGTTT TCAGGCAAGA GAATGTGGAG GATTATTATG ATATAGGAGA GGAGCTTGGA 
AGCGGTCAGT TTGCTGTGGT GAAAAAATGC CAAGAAAAGA GCACAGGCGT GCATTATGCT 
GCAAAATTTA TCAAGAAGCG AAGGACCAAA TCAAGTCGGC GTGGGGTGAC CCGGGAGGAT 
ATAGAGAGGG AGGTCAGCAT ACTGAAGGAG ATCCAGCACC CCAATGTGAT CACGTTGCAC 
GAAGTGTACG AGAATAAAGC AGATGTCATT CTCATTCTGG AGCTAGTTGC AGGAGGTGAG 
CTATTTGACT TTCTTGCTGA AAAGGAGTCT CTGACAGAGG AGGAAGCTAC AGAGTTCATC 
AAGCAGATCC TTAATGGTGT GAATTACCTC CACTCCCTGC ACATTGCCCA CTTTGACCTC 
AAGCCTGAAA ACATCATGCT GCTTGATAGA AACGCACCAA ATCCTCGTAT TAAGATCATA 
GATTTTGGCT TGGCTCACAG AATCGACTCT GGAAATGAAT TTAAGAACAT ATTCGGAACT 
CCAGAATTTG TTGCGCCTGA AATTGTAAAT TATGAACCGC TTGGTCTTGA ATCTGATATG 
TGGAGTATTG GAGTCATAAC ATACATTCTA TTGAGCGGGG CATCTCCATT CCTTGGGGAA 
ACAAAGCAGG AGACCTTGGC TAATATATCT GCTGTCAGTT ATGACTTTGA GGAGGAGTTC 
TTTAGTAGTA CAAGTGACCT GGCCAAAGAT TTTATAAGGC GGCTGCTAGT CAAGGACCCA 
AAAAAAAGGA TGACCATTCA AGACAGTTTG TACCACCCTT GGATTAAGCC AAAAGATACT 
CAGCAAGCAC TGAGCAGGAA AGCCTCTGCA GTCAATATGG AGAAGTTTAA GAAGTTTGCT 
GCTCGCAGAA AATGGAAACA ATCTGTCCGT TTGATATCCC TGTGCCAAAG ACTGTCAAGA 
TCCTTCTTGT CCAGGAGCAA CATGAGTATT GCTAGGAGTG ATGACACACT GGATGAGGAG 
GACTCTTTTG TAATGAAAGC CATCATTCAT GCCATAAATG ACGATAACGT TCCTGGTTTG 
CAGCATCTTT TTGGGTCTTT GTCAAACTAT GATGTGAACC AGCCAAACAA GCATGGCACC 
CCTCCCTTGT TAATTGCTGC TGGCTGTGGG AATATTCAGA TCCTTGAACT GCTCATAAAG 
CGTGGCGCAC AGATTGATGT ACAAGATAAG GCTGGGTCCA ATGCAATTTA CTGGGCTTCT 
CGTCATGGTC ACGTTGAGAC TCTGAAATTT CTACATCAGA ATAACTGTCC TTTAGATATT 
AAAGATAAGT CAGGAGAAAC AGCACTTCAC GTGGCGGCCC GTTATGGACA TGCTGAAGTT 
ATTCAATACT TGTGCAGCAT TGGATCACAT CCAGACATCC AGGATAAGGA GGAAGAAACC 
CCTCTGCATT GTGCTGCTTG GCATGGTTAC TACCCAGTGG CAAAAGCTCT GTGCCAGGCT 
GGCTGCAATG TCAACATTCG CAACAGGGAA GGGGAAACAC CCATTCTGAC AGCAGCAGCA 
AGAGGGTACC ACGATATTGT GGAATGCTTG GTTGACCATG GAGGCGACCT TGATGCAACA 
GACAAGGATG GGCACATAGC ACTCCACCTT GCAGTGAGAC GGTGTCAGAT GGAAGTAGTG 
AAAACGCTCA TCATCCATGG ATGCTTTGTA GACTTCCAGG ATCGCCATGG TAACACACCT 
CTGCATGTTG CCTGCAAAGA TGGGAATCTT CCTATTGTAA TGGCACTGTG TGAGGCAAGA 
TGTAATTTGG ATATAACTAA CAAGTATGGC CGAACACCTT TACATCTGGC AGCAAACAAT 
GGTATAGTGG AAGTTGTCCG ATACCTCTGC GTCGCTGGTG CAAATGTTGA AGCCTTGACT 
TTGGATGGGA AAACAGCAGA GGATCTCGCT AGGTCAGAAC AGCATGAGTC CGTTGTCAGT 
CTGCTCGCAA GGCTTCGAAG GGATACCTAC AGAACTTTGT TCATTCAGCA GCTCAGACCT 
TCACCAACCC CCCAGCCAAG AATTAAACTG AAGTTATTTG GACACATGGG ATCAGGAAAG 
ACCACGCTTG TAGAGTCCCT CAAATGTGGC CTTATCCGAA GCTTCTTCAG AAGACGCAGA 
CCTCGTCTTT CATCAACTAA CTCTTCCCGT TTTCCTTCTT CTCCACTAAC AGCTAAGACA 
GTTTCAGTGA GCATCTCGGA TCTCTACCCT GGTTGTGAGA ATGTTAGTGT AAGAAGTCGG 
AGTATGATGT TTGAGCCAGG ACTAACCAAA GGGGTTCTTG AAGTATTTGT TTCACCGTCC 
CCTCACTGTT ACACAGATGA CCAGTCAACC AAGGCTATAG ACATCCAGAG TGCCAATCTT 
AACGGTGTTG GAGATTTTAG TGTGTGGGAA TTCTCTGGAA ACCCAACGTA TTATTGCAGC 
TATGATTATT TTGCTGCCAA TGATCCCACA TCAGTTCACA TTGTAGTGTT TAGCCTTGAA 
GAACCCTATG AGATTCAGCT GAATCAAGTG ATATTCTGGC TCAATTTCCT AAAGTCACTT 
GTTCCTGTGG AAGAACCAAT AGCGTATGGT GGGAAGCTGA AGAATCTACT CCACGTTGTG 
TTGGTGGCCA CACATGCTGA TATTGTTAAC CTTCCTCGCC CTGCCGGAGG AGAATTTGGT 
TACGACAAAA GTCTAGCATT GCTGAAAGAA GTAAGAAATA GATTTGGAAA CGATCTCCAG 
ATTTTGGATA GACTGTTTGT TCTGGATGCC GGGGGATCAG GGTCCAAGGA GATGAAGCTT 
CTGAGGAATC ACCTCCAGGA ATTAAGAAGC CAGCTGATAT CTGACTGTCC ACCTATGACA 
CCGCTTTGTG AGAAGGTGAT GTCAACTCTT CCATCCTGGA GAAAACAGAA TGGTCCCAAC 
CAACTTCTTT CTCTTCAACA ATTTGTATAT GATGTCCAGG ACCAACTAAA TCCACTAGCA 
AGTGAAGAGG ATGTTAGAAA CCTTGCATTG CAACTGCACA GTATTGGAGA AGTTAATATA 
ATGCAGAGTG AGACCGTCCA AGATGTTGTG CTTCTAGATC CACGATGGCT GTGTCACAAT 
GTCCTCGGAA AGCTACTCTC TGTAGAAAAC CCAAAAGCCC TACACCATTA CAGGGGAAGG 
TATACAATGG AAGATATTCA GCGCTTGGTC ACCGACAGTG ATGTTGATGA ACTTGTGCAG 
ATCCTGGATG CAATGGACAT TTGTGCCCGA GATCTAAGCA GCGGAGCTAT GGTGGACATC 
CCTGCCCTCA TTAAAACAGA CAACCTCCAC CGATCATGGG CTGATGAAGA AGAGGACATA 
ATGATTTATG GAGGTGTTAG AATTGTTCCT GTGGAACACC TTACTCCCTT TCCGTGTGGA 
ATTTTTCACA AAGTTCAAGT TAATTTATGC CGATGGGTAC ATCAACAGAG CGCCGACGGT 
GATGCTGATA TACGTTTATG GGTAAACGGT TGTAAAATAG CAAATCGAGG GGCAGAGGTT 
TTGGTGTTGA TGGTCAACCA TGGTCAAGGA ATTGAAGTGC AAGTTAGGGG TCTGGAAACT 
GAGAAAATCA AGTGTTGTCT ACTTCTTGAA TCCATTTGCA GTATCATTGA CAACTTATTT 
GCAACAACGT TGCCTGGTCT TTTAACTGTA AAACATTACT TGAGCCCTCA ACAGCTAAGA 
GAGCATCACG AGCCACTGAT GGTTTACCAA CCTAGAGACT TTTTTCGGGC CCAAATACAA 
AAGGAGACTT CACTGGCTAA CACCATGGCT GGATACAAAG AGAGCTTTAG CAGCATTCTT 
TGTTTTGGGT GCCTGGATGT TTACTCTCAA GGAAACCTAG GCCTGGATCA CCATGTGTCC 
GATATCAATA TTCTTGTTAG AAGAAAGCTC AGCCGACTTT TAGACCCACC AGATCCCATG 
GGAAAGGACT GGTGTCTACT AGCTATGAAT TTGGGCCTAC CTGACTTAGT TGCCAAATAC 
AATACTAACA ATTCTGTACA AAAAGACTTT TTGCCTAGTC CAATGTGTGC TCTTCTGCAG 
GAATGGAGCA ATTCTCCTGA AAGTACTGTA TCGCTCCTAA TGTCTAAACT GAGAGAACTT 
GGGCGCAGGG ATGCAGCAGA TTTTTTACTA AAAGCATCCT CTGTTTTTAA GGTGACTCTT 
GATATCCATG GACAAGAAGC CTATGCGGCA AGCTGCAACA GCGGCACATC CTATAATTCA 
ATTAGCTCAG TCGTGTCACG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001116911.1
CDS	243..4526
Translation	MTVFRQENVEDYYDIGEELGSGQFAVVKKCQEKSTGVHYAAKFIKKRRTKSSRRGVTREDIEREVSILKEIQHPNVITLHEVYENKADVILILELVAGGELFDFLAEKESLTEEEATEFIKQILNGVNYLHSLHIAHFDLKPENIMLLDRNAPNPRIKIIDFGLAHRIDSGNEFKNIFGTPEFVAPEIVNYEPLGLESDMWSIGVITYILLSGASPFLGETKQETLANISAVSYDFEEEFFSSTSDLAKDFIRRLLVKDPKKRMTIQDSLYHPWIKPKDTQQALSRKASAVNMEKFKKFAARRKWKQSVRLISLCQRLSRSFLSRSNMSIARSDDTLDEEDSFVMKAIIHAINDDNVPGLQHLFGSLSNYDVNQPNKHGTPPLLIAAGCGNIQILELLIKRGAQIDVQDKAGSNAIYWASRHGHVETLKFLHQNNCPLDIKDKSGETALHVAARYGHAEVIQYLCSIGSHPDIQDKEEETPLHCAAWHGYYPVAKALCQAGCNVNIRNREGETPILTAAARGYHDIVECLVDHGGDLDATDKDGHIALHLAVRRCQMEVVKTLIIHGCFVDFQDRHGNTPLHVACKDGNLPIVMALCEARCNLDITNKYGRTPLHLAANNGIVEVVRYLCVAGANVEALTLDGKTAEDLARSEQHESVVSLLARLRRDTYRTLFIQQLRPSPTPQPRIKLKLFGHMGSGKTTLVESLKCGLIRSFFRRRRPRLSSTNSSRFPSSPLTAKTVSVSISDLYPGCENVSVRSRSMMFEPGLTKGVLEVFVSPSPHCYTDDQSTKAIDIQSANLNGVGDFSVWEFSGNPTYYCSYDYFAANDPTSVHIVVFSLEEPYEIQLNQVIFWLNFLKSLVPVEEPIAYGGKLKNLLHVVLVATHADIVNLPRPAGGEFGYDKSLALLKEVRNRFGNDLQILDRLFVLDAGGSGSKEMKLLRNHLQELRSQLISDCPPMTPLCEKVMSTLPSWRKQNGPNQLLSLQQFVYDVQDQLNPLASEEDVRNLALQLHSIGEVNIMQSETVQDVVLLDPRWLCHNVLGKLLSVENPKALHHYRGRYTMEDIQRLVTDSDVDELVQILDAMDICARDLSSGAMVDIPALIKTDNLHRSWADEEEDIMIYGGVRIVPVEHLTPFPCGIFHKVQVNLCRWVHQQSADGDADIRLWVNGCKIANRGAEVLVLMVNHGQGIEVQVRGLETEKIKCCLLLESICSIIDNLFATTLPGLLTVKHYLSPQQLREHHEPLMVYQPRDFFRAQIQKETSLANTMAGYKESFSSILCFGCLDVYSQGNLGLDHHVSDINILVRRKLSRLLDPPDPMGKDWCLLAMNLGLPDLVAKYNTNNSVQKDFLPSPMCALLQEWSNSPESTVSLLMSKLRELGRRDAADFLLKASSVFKVTLDIHGQEAYAASCNSGTSYNSISSVVSR

Target ORF information:

RefSeq Version	NM_001123439.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis death associated protein kinase 1 (dapk1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001123439.1

ORF Insert Sequence:

ATGACTGTTT TCAGGCAAGA GAATGTGGAG GATTATTATG ATATAGGAGA GGAGCTTGGA 
AGCGGTCAGT TTGCTGTGGT GAAAAAATGC CAAGAAAAGA GCACAGGCGT GCATTATGCT 
GCAAAATTTA TCAAGAAGCG AAGGACCAAA TCAAGTCGGC GTGGGGTGAC CCGGGAGGAT 
ATAGAGAGGG AGGTCAGCAT ACTGAAGGAG ATCCAGCACC CCAATGTGAT CACGTTGCAC 
GAAGTGTACG AGAATAAAGC AGATGTCATT CTCATTCTGG AGCTAGTTGC AGGAGGTGAG 
CTATTTGACT TTCTTGCTGA AAAGGAGTCT CTGACAGAGG AGGAAGCTAC AGAGTTCATC 
AAGCAGATCC TTAATGGTGT GAATTACCTC CACTCCCTGC ACATTGCCCA CTTTGACCTC 
AAGCCTGAAA ACATCATGCT GCTTGATAGA AACGCACCAA ATCCTCGTAT TAAGATCATA 
GATTTTGGCT TGGCTCACAG AATCGACTCT GGAAATGAAT TTAAGAACAT ATTCGGAACT 
CCAGAATTTG TTGCGCCTGA AATTGTAAAT TATGAACCGC TTGGTCTTGA ATCTGATATG 
TGGAGTATTG GAGTCATAAC ATACATTCTA TTGAGCGGGG CATCTCCATT CCTTGGGGAA 
ACAAAGCAGG AGACCTTGGC TAATATATCT GCTGTCAGTT ATGACTTTGA GGAGGAGTTC 
TTTAGTAGTA CAAGTGACCT GGCCAAAGAT TTTATAAGGC GGCTGCTAGT CAAGGACCCA 
AAAAAAAGGA TGACCATTCA AGACAGTTTG TACCACCCTT GGATTAAGCC AAAAGATACT 
CAGCAAGCAC TGAGCAGGAA AGCCTCTGCA GTCAATATGG AGAAGTTTAA GAAGTTTGCT 
GCTCGCAGAA AATGGAAACA ATCTGTCCGT TTGATATCCC TGTGCCAAAG ACTGTCAAGA 
TCCTTCTTGT CCAGGAGCAA CATGAGTATT GCTAGGAGTG ATGACACACT GGATGAGGAG 
GACTCTTTTG TAATGAAAGC CATCATTCAT GCCATAAATG ACGATAACGT TCCTGGTTTG 
CAGCATCTTT TTGGGTCTTT GTCAAACTAT GATGTGAACC AGCCAAACAA GCATGGCACC 
CCTCCCTTGT TAATTGCTGC TGGCTGTGGG AATATTCAGA TCCTTGAACT GCTCATAAAG 
CGTGGCGCAC AGATTGATGT ACAAGATAAG GCTGGGTCCA ATGCAATTTA CTGGGCTTCT 
CGTCATGGTC ACGTTGAGAC TCTGAAATTT CTACATCAGA ATAACTGTCC TTTAGATATT 
AAAGATAAGT CAGGAGAAAC AGCACTTCAC GTGGCGGCCC GTTATGGACA TGCTGAAGTT 
ATTCAATACT TGTGCAGCAT TGGATCACAT CCAGACATCC AGGATAAGGA GGAAGAAACC 
CCTCTGCATT GTGCTGCTTG GCATGGTTAC TACCCAGTGG CAAAAGCTCT GTGCCAGGCT 
GGCTGCAATG TCAACATTCG CAACAGGGAA GGGGAAACAC CCATTCTGAC AGCAGCAGCA 
AGAGGGTACC ACGATATTGT GGAATGCTTG GTTGACCATG GAGGCGACCT TGATGCAACA 
GACAAGGATG GGCACATAGC ACTCCACCTT GCAGTGAGAC GGTGTCAGAT GGAAGTAGTG 
AAAACGCTCA TCATCCATGG ATGCTTTGTA GACTTCCAGG ATCGCCATGG TAACACACCT 
CTGCATGTTG CCTGCAAAGA TGGGAATCTT CCTATTGTAA TGGCACTGTG TGAGGCAAGA 
TGTAATTTGG ATATAACTAA CAAGTATGGC CGAACACCTT TACATCTGGC AGCAAACAAT 
GGTATAGTGG AAGTTGTCCG ATACCTCTGC GTCGCTGGTG CAAATGTTGA AGCCTTGACT 
TTGGATGGGA AAACAGCAGA GGATCTCGCT AGGTCAGAAC AGCATGAGTC CGTTGTCAGT 
CTGCTCGCAA GGCTTCGAAG GGATACCTAC AGAACTTTGT TCATTCAGCA GCTCAGACCT 
TCACCAACCC CCCAGCCAAG AATTAAACTG AAGTTATTTG GACACATGGG ATCAGGAAAG 
ACCACGCTTG TAGAGTCCCT CAAATGTGGC CTTATCCGAA GCTTCTTCAG AAGACGCAGA 
CCTCGTCTTT CATCAACTAA CTCTTCCCGT TTTCCTTCTT CTCCACTAAC AGCTAAGACA 
GTTTCAGTGA GCATCTCGGA TCTCTACCCT GGTTGTGAGA ATGTTAGTGT AAGAAGTCGG 
AGTATGATGT TTGAGCCAGG ACTAACCAAA GGGGTTCTTG AAGTATTTGT TTCACCGTCC 
CCTCACTGTT ACACAGATGA CCAGTCAACC AAGGCTATAG ACATCCAGAG TGCCAATCTT 
AACGGTGTTG GAGATTTTAG TGTGTGGGAA TTCTCTGGAA ACCCAACGTA TTATTGCAGC 
TATGATTATT TTGCTGCCAA TGATCCCACA TCAGTTCACA TTGTAGTGTT TAGCCTTGAA 
GAACCCTATG AGATTCAGCT GAATCAAGTG ATATTCTGGC TCAATTTCCT AAAGTCACTT 
GTTCCTGTGG AAGAACCAAT AGCGTATGGT GGGAAGCTGA AGAATCTACT CCACGTTGTG 
TTGGTGGCCA CACATGCTGA TATTGTTAAC CTTCCTCGCC CTGCCGGAGG AGAATTTGGT 
TACGACAAAA GTCTAGCATT GCTGAAAGAA GTAAGAAATA GATTTGGAAA CGATCTCCAG 
ATTTTGGATA GACTGTTTGT TCTGGATGCC GGGGGATCAG GGTCCAAGGA GATGAAGCTT 
CTGAGGAATC ACCTCCAGGA ATTAAGAAGC CAGCTGATAT CTGACTGTCC ACCTATGACA 
CCGCTTTGTG AGAAGGTGAT GTCAACTCTT CCATCCTGGA GAAAACAGAA TGGTCCCAAC 
CAACTTCTTT CTCTTCAACA ATTTGTATAT GATGTCCAGG ACCAACTAAA TCCACTAGCA 
AGTGAAGAGG ATGTTAGAAA CCTTGCATTG CAACTGCACA GTATTGGAGA AGTTAATATA 
ATGCAGAGTG AGACCGTCCA AGATGTTGTG CTTCTAGATC CACGATGGCT GTGTCACAAT 
GTCCTCGGAA AGCTACTCTC TGTAGAAAAC CCAAAAGCCC TACACCATTA CAGGGGAAGG 
TATACAATGG AAGATATTCA GCGCTTGGTC ACCGACAGTG ATGTTGATGA ACTTGTGCAG 
ATCCTGGATG CAATGGACAT TTGTGCCCGA GATCTAAGCA GCGGAGCTAT GGTGGACATC 
CCTGCCCTCA TTAAAACAGA CAACCTCCAC CGATCATGGG CTGATGAAGA AGAGGACATA 
ATGATTTATG GAGGTGTTAG AATTGTTCCT GTGGAACACC TTACTCCCTT TCCGTGTGGA 
ATTTTTCACA AAGTTCAAGT TAATTTATGC CGATGGGTAC ATCAACAGAG CGCCGACGGT 
GATGCTGATA TACGTTTATG GGTAAACGGT TGTAAAATAG CAAATCGAGG GGCAGAGGTT 
TTGGTGTTGA TGGTCAACCA TGGTCAAGGA ATTGAAGTGC AAGTTAGGGG TCTGGAAACT 
GAGAAAATCA AGTGTTGTCT ACTTCTTGAA TCCATTTGCA GTATCATTGA CAACTTATTT 
GCAACAACGT TGCCTGGTCT TTTAACTGTA AAACATTACT TGAGCCCTCA ACAGCTAAGA 
GAGCATCACG AGCCACTGAT GGTTTACCAA CCTAGAGACT TTTTTCGGGC CCAAATACAA 
AAGGAGACTT CACTGGCTAA CACCATGGCT GGATACAAAG AGAGCTTTAG CAGCATTCTT 
TGTTTTGGGT GCCTGGATGT TTACTCTCAA GGAAACCTAG GCCTGGATCA CCATGTGTCC 
GATATCAATA TTCTTGTTAG AAGAAAGCTC AGCCGACTTT TAGACCCACC AGATCCCATG 
GGAAAGGACT GGTGTCTACT AGCTATGAAT TTGGGCCTAC CTGACTTAGT TGCCAAATAC 
AATACTAACA ATTCTGTACA AAAAGACTTT TTGCCTAGTC CAATGTGTGC TCTTCTGCAG 
GAATGGAGCA ATTCTCCTGA AAGTACTGTA TCGCTCCTAA TGTCTAAACT GAGAGAACTT 
GGGCGCAGGG ATGCAGCAGA TTTTTTACTA AAAGCATCCT CTGTTTTTAA GGTGACTCTT 
GATATCCATG GACAAGAAGC CTATGCGGCA AGCTGCAACA GCGGCACATC CTATAATTCA 
ATTAGCTCAG TCGTGTCACG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dapk1 ( XM_012955218.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012810672.1 Xenopus tropicalis death-associated protein kinase 1 (dapk1), transcript variant X1, mRNA.

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CloneID	OXa31370
Clone ID Related Accession (Same CDS sequence)	XM_012955218.2 , XM_012955236.2
Accession Version	XM_012955218.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4284bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	death-associated protein kinase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012955218.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGTTT TCAGGCAAGA GAATGTGGAG GATTATTATG ATATAGGAGA GGAGCTTGGA 
AGCGGTCAGT TTGCTGTGGT GAAAAAATGC CAAGAAAAGA GCACAGGCGT GCATTATGCT 
GCAAAATTTA TCAAGAAGCG AAGGACCAAA TCAAGTCGGC GTGGGGTGAC CCGGGAGGAT 
ATAGAGAGGG AGGTCAGCAT ACTGAAGGAG ATCCAGCACC CCAATGTGAT CACGTTGCAC 
GAAGTGTACG AGAATAAAGC AGATGTCATT CTCATTCTGG AGCTAGTTGC AGGAGGTGAG 
CTATTTGACT TTCTTGCTGA AAAGGAGTCT CTGACAGAGG AGGAAGCTAC AGAGTTCATC 
AAGCAGATCC TTAATGGTGT GAATTACCTC CACTCCCTGC ACATTGCCCA CTTTGACCTC 
AAGCCTGAAA ACATCATGCT GCTTGATAGA AACGCACCAA ATCCTCGTAT TAAGATCATA 
GATTTTGGCT TGGCTCACAG AATCGACTCT GGAAATGAAT TTAAGAACAT ATTCGGAACT 
CCAGAATTTG TTGCGCCTGA AATTGTAAAT TATGAACCGC TTGGTCTTGA ATCTGATATG 
TGGAGTATTG GAGTCATAAC ATACATTCTA TTGAGCGGGG CATCTCCATT CCTTGGGGAA 
ACAAAGCAGG AGACCTTGGC TAATATATCT GCTGTCAGTT ATGACTTTGA GGAGGAGTTC 
TTTAGTAGTA CAAGTGACCT GGCCAAAGAT TTTATAAGGC GGCTGCTAGT CAAGGACCCA 
AAAAAAAGGA TGACCATTCA AGACAGTTTG TGCCACCCTT GGATTAAGCC AAAAGATACT 
CAGCAAGCAC TGAGCAGGAA AGCCTCTGCA GTCAATATGG AGAAGTTTAA GAAGTTTGCT 
GCTCGCAGAA AATGGAAACA ATCTGTCCGT TTGATATCCC TGTGCCAAAG ACTGTCAAGA 
TCCTTCTTGT CCAGGAGCAA CATGAGTATT GCTAGGAGTG ATGACACACT GGATGAGGAG 
GACTCTTTTG TAATGAAAGC CATCATTCAT GCCATAAATG ACGATAACGT TCCTGGTTTG 
CAGCATCTTT TTGGGTCTTT GTCAAACTAT GATGTGAACC AGCCAAACAA GCATGGCACC 
CCTCCCTTGT TAATTGCTGC TGGCTGTGGG AATATTCAGA TCCTTGAACT GCTCATAAAG 
CGTGGCGCAC AGATTGATGT ACAAGATAAG GCTGGGTCCA ATGCAATTTA CTGGGCTTCT 
CGTCATGGTC ACGTTGAGAC TCTGAAATTT CTACATCAGA ATAACTGTCC TTTAGATATT 
AAAGATAAGT CAGGAGAAAC AGCACTTCAC GTGGCGGCCC GTTATGGACA TGCTGAAGTT 
ATTCAATACT TGTGCAGCAT TGGATCACAT CCAGACATCC AAGATAAGGA GGAAGAAACC 
CCTCTGCATT GTGCTGCTTG GCATGGTTAC TACCCAGTGG CAAAAGCTCT GTGCCAGGCT 
GGCTGCAATG TCAACATTCG CAACAGGGAA GGGGAAACAC CCATTCTGAC AGCAGCAGCA 
AGAGGGTACC ACGATATTGT GGAATGCTTG GTTGACCATG GAGGCGACCT TGATGCAACA 
GACAAGGATG GGCACATAGC ACTCCACCTT GCAGTGAGAC GGTGTCAGAT GGAAGTAGTG 
AAAACGCTCA TCATCCATGG ATGCTTTGTA GACTTCCAGG ATCGCCATGG TAACACACCT 
CTGCATGTTG CCTGCAAAGA TGGGAATCTT CCTATTGTAA TGGCACTGTG TGAGGCAAGA 
TGTAATTTGG ATATAACTAA CAAGTATGGC CGAACACCTT TACATCTGGC AGCAAACAAT 
GGTATAGTGG AAGTTGTCCG ATACCTCTGC GTCGCTGGTG CAAATGTTGA AGCCTTGACT 
TTGGATGGGA AAACAGCAGA GGATCTCGCT AGGTCAGAAC AGCATGAGTC CGTTGTCAGT 
CTGCTCGCAA GGCTTCGAAG GGATACCTAC AGAACTTTGT TCATTCAGCA GCTCAGACCT 
TCACCAACCC CCCAGCCAAG AATTAAACTG AAGTTATTTG GACACATGGG ATCAGGAAAG 
ACCACGCTTG TAGAGTCCCT CAAATGTGGC CTTATCCGAA GCTTCTTCAG AAGACGCAGA 
CCTCGTCTTT CATCAACTAA CTCTTCCCGT TTTCCTTCTT CTCCACTAAC AGCTAAGACA 
GTTTCAGTGA GCATCTCGGA TCTCTACCCT GGTTGTGAGA ATGTTAGTGT AAGAAGTCGG 
AGTATGATGT TTGAGCCAGG TCTAACCAAA GGGGTTCTTG AAGTATTTGT TTCACCGTCC 
CCTCACTGTT ACACAGATGA CCAGTCAACC AAGGCTATAG ACATCCAGAG TGCCAATCTT 
AACGGTGTTG GAGATTTTAG TGTGTGGGAA TTCTCTGGAA ACCCAACGTA TTATTGCAGC 
TATGATTATT TTGCTGCCAA TGATCCCACA TCAGTTCACA TTGTAGTGTT TAGCCTTGAA 
GAACCCTATG AGATTCAGCT GAATCAAGTG ATATTCTGGC TCAATTTCCT AAAGTCACTT 
GTTCCTGTGG AAGAACCAAT AGCGTATGGT GGGAAGCTGA AGAATCTACT CCACGTTGTG 
TTGGTGGCCA CACATGCTGA TATTGTTAAC CTTCCTCGCC CTGCCGGAGG AGAATTTGGT 
TACGACAAAA GTCTAGCATT GCTGAAAGAA GTAAGAAATA GATTTGGAAA CGATCTCCAG 
ATTTTGGATA GACTGTTTGT TCTGGATGCC GGGGGATCAG GGTCCAAGGA GATGAAGCTT 
CTGAGGAATC ACCTCCAGGA ATTAAGAAGC CAGCTGATAT CTGACTGTCC ACCTATGACA 
CCGCTTTGTG AGAAGGTGAT GTCAACTCTT CCATCCTGGA GAAAACAGAA TGGTCCCAAT 
CAACTTCTTT CTCTTCAACA ATTTGTATAT GATGTCCAGG ACCAACTAAA TCCACTAGCA 
AGTGAAGAGG ATGTTAGAAA CCTTGCATTG CAACTGCACA GTATTGGAGA AGTTAATATA 
ATGCAGAGTG AGACCGTCCA AGATGTTGTG CTTCTAGATC CACGATGGCT GTGTCACAAT 
GTCCTCGGAA AGCTACTCTC TGTAGAAAAC CCAAAAGCCC TACACCATTA CAGGGGAAGG 
TATACAATGG AAGATATTCA GCGCTTGGTC ACCGACAGTG ATGTTGATGA ACTTGTGCAG 
ATCCTGGATG CAATGGACAT TTGTGCCCGA GATCTAAGCA GCGGAGCTAT GGTGGACATC 
CCTGCCCTCA TTAAAACAGA CAACCTCCAC CGATCATGGG CTGATGAAGA AGAGGACATA 
ATGATTTATG GAGGTGTTAG AATTGTTCCT GTGGAACACC TTACTCCCTT TCCGTGTGGA 
ATTTTTCACA AAGTTCAAGT TAATTTATGC CGATGGGTAC ATCAACAGAG CGCCGACGGT 
GATGCTGATA TACGTTTATG GGTAAACGGT TGTAAAATAG CAAATCGAGG GGCAGAGGTT 
TTGGTGTTGA TGGTCAACCA TGGTCAAGGA ATTGAAGTGC AAGTTAGGGG TCTGGAAACT 
GAGAAAATCA AGTGTTGTCT ACTTCTTGAA TCCATTTGCA GTATCATTGA CAACTTATTT 
GCAACAACGT TGCCTGGTCT TTTAACTGTA AAACATTACT TGAGCCCTCA ACAGCTAAGA 
GAGCATCACG AGCCACTGAT GGTTTACCAA CCTAGAGACT TTTTTCGGGC CCAAATACAA 
AAGGAGACTT CACTGGCTAA CACCATGGCT GGATACAAAG AGAGCTTTAG CAGCATTCTT 
TGTTTTGGGT GCCTGGATGT TTACTCTCAA GGAAACCTAG GCCTGGATCA CCATGTGTCC 
GATATCAATA TTCTTGTTAG AAGAAAGCTC AGCCGACTTT TAGACCCACC AGATCCCATG 
GGAAAGGACT GGTGTCTACT AGCTATGAAT TTGGGCCTAC CTGACTTAGT TGCCAAATAC 
AATACTAACA ATTCTGTACA AAAAGACTTT TTGCCTAGTC CAATGTGTGC TCTTCTGCAG 
GAATGGAGCA ATTCTCCTGA AAGTACTGTA TCGCTCCTAA TGTCTAAACT GAGAGAACTT 
GGGCGCAGGG ATGCAGCAGA TTTTTTACTA AAAGCATCCT CTGTTTTTAA GGTGACTCTT 
GATATCCATG GACAAGAAGC CTATGCGGCA AGCTGCAACA GCGGCACATC CTATAATTCA 
ATTAGCTCAG TCGTGTCACG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012810672.1
CDS	871..5154
Translation	MTVFRQENVEDYYDIGEELGSGQFAVVKKCQEKSTGVHYAAKFIKKRRTKSSRRGVTREDIEREVSILKEIQHPNVITLHEVYENKADVILILELVAGGELFDFLAEKESLTEEEATEFIKQILNGVNYLHSLHIAHFDLKPENIMLLDRNAPNPRIKIIDFGLAHRIDSGNEFKNIFGTPEFVAPEIVNYEPLGLESDMWSIGVITYILLSGASPFLGETKQETLANISAVSYDFEEEFFSSTSDLAKDFIRRLLVKDPKKRMTIQDSLCHPWIKPKDTQQALSRKASAVNMEKFKKFAARRKWKQSVRLISLCQRLSRSFLSRSNMSIARSDDTLDEEDSFVMKAIIHAINDDNVPGLQHLFGSLSNYDVNQPNKHGTPPLLIAAGCGNIQILELLIKRGAQIDVQDKAGSNAIYWASRHGHVETLKFLHQNNCPLDIKDKSGETALHVAARYGHAEVIQYLCSIGSHPDIQDKEEETPLHCAAWHGYYPVAKALCQAGCNVNIRNREGETPILTAAARGYHDIVECLVDHGGDLDATDKDGHIALHLAVRRCQMEVVKTLIIHGCFVDFQDRHGNTPLHVACKDGNLPIVMALCEARCNLDITNKYGRTPLHLAANNGIVEVVRYLCVAGANVEALTLDGKTAEDLARSEQHESVVSLLARLRRDTYRTLFIQQLRPSPTPQPRIKLKLFGHMGSGKTTLVESLKCGLIRSFFRRRRPRLSSTNSSRFPSSPLTAKTVSVSISDLYPGCENVSVRSRSMMFEPGLTKGVLEVFVSPSPHCYTDDQSTKAIDIQSANLNGVGDFSVWEFSGNPTYYCSYDYFAANDPTSVHIVVFSLEEPYEIQLNQVIFWLNFLKSLVPVEEPIAYGGKLKNLLHVVLVATHADIVNLPRPAGGEFGYDKSLALLKEVRNRFGNDLQILDRLFVLDAGGSGSKEMKLLRNHLQELRSQLISDCPPMTPLCEKVMSTLPSWRKQNGPNQLLSLQQFVYDVQDQLNPLASEEDVRNLALQLHSIGEVNIMQSETVQDVVLLDPRWLCHNVLGKLLSVENPKALHHYRGRYTMEDIQRLVTDSDVDELVQILDAMDICARDLSSGAMVDIPALIKTDNLHRSWADEEEDIMIYGGVRIVPVEHLTPFPCGIFHKVQVNLCRWVHQQSADGDADIRLWVNGCKIANRGAEVLVLMVNHGQGIEVQVRGLETEKIKCCLLLESICSIIDNLFATTLPGLLTVKHYLSPQQLREHHEPLMVYQPRDFFRAQIQKETSLANTMAGYKESFSSILCFGCLDVYSQGNLGLDHHVSDINILVRRKLSRLLDPPDPMGKDWCLLAMNLGLPDLVAKYNTNNSVQKDFLPSPMCALLQEWSNSPESTVSLLMSKLRELGRRDAADFLLKASSVFKVTLDIHGQEAYAASCNSGTSYNSISSVVSR

Target ORF information:

RefSeq Version	XM_012955218.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis death-associated protein kinase 1 (dapk1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012955218.2

ORF Insert Sequence:

ATGACTGTTT TCAGGCAAGA GAATGTGGAG GATTATTATG ATATAGGAGA GGAGCTTGGA 
AGCGGTCAGT TTGCTGTGGT GAAAAAATGC CAAGAAAAGA GCACAGGCGT GCATTATGCT 
GCAAAATTTA TCAAGAAGCG AAGGACCAAA TCAAGTCGGC GTGGGGTGAC CCGGGAGGAT 
ATAGAGAGGG AGGTCAGCAT ACTGAAGGAG ATCCAGCACC CCAATGTGAT CACGTTGCAC 
GAAGTGTACG AGAATAAAGC AGATGTCATT CTCATTCTGG AGCTAGTTGC AGGAGGTGAG 
CTATTTGACT TTCTTGCTGA AAAGGAGTCT CTGACAGAGG AGGAAGCTAC AGAGTTCATC 
AAGCAGATCC TTAATGGTGT GAATTACCTC CACTCCCTGC ACATTGCCCA CTTTGACCTC 
AAGCCTGAAA ACATCATGCT GCTTGATAGA AACGCACCAA ATCCTCGTAT TAAGATCATA 
GATTTTGGCT TGGCTCACAG AATCGACTCT GGAAATGAAT TTAAGAACAT ATTCGGAACT 
CCAGAATTTG TTGCGCCTGA AATTGTAAAT TATGAACCGC TTGGTCTTGA ATCTGATATG 
TGGAGTATTG GAGTCATAAC ATACATTCTA TTGAGCGGGG CATCTCCATT CCTTGGGGAA 
ACAAAGCAGG AGACCTTGGC TAATATATCT GCTGTCAGTT ATGACTTTGA GGAGGAGTTC 
TTTAGTAGTA CAAGTGACCT GGCCAAAGAT TTTATAAGGC GGCTGCTAGT CAAGGACCCA 
AAAAAAAGGA TGACCATTCA AGACAGTTTG TGCCACCCTT GGATTAAGCC AAAAGATACT 
CAGCAAGCAC TGAGCAGGAA AGCCTCTGCA GTCAATATGG AGAAGTTTAA GAAGTTTGCT 
GCTCGCAGAA AATGGAAACA ATCTGTCCGT TTGATATCCC TGTGCCAAAG ACTGTCAAGA 
TCCTTCTTGT CCAGGAGCAA CATGAGTATT GCTAGGAGTG ATGACACACT GGATGAGGAG 
GACTCTTTTG TAATGAAAGC CATCATTCAT GCCATAAATG ACGATAACGT TCCTGGTTTG 
CAGCATCTTT TTGGGTCTTT GTCAAACTAT GATGTGAACC AGCCAAACAA GCATGGCACC 
CCTCCCTTGT TAATTGCTGC TGGCTGTGGG AATATTCAGA TCCTTGAACT GCTCATAAAG 
CGTGGCGCAC AGATTGATGT ACAAGATAAG GCTGGGTCCA ATGCAATTTA CTGGGCTTCT 
CGTCATGGTC ACGTTGAGAC TCTGAAATTT CTACATCAGA ATAACTGTCC TTTAGATATT 
AAAGATAAGT CAGGAGAAAC AGCACTTCAC GTGGCGGCCC GTTATGGACA TGCTGAAGTT 
ATTCAATACT TGTGCAGCAT TGGATCACAT CCAGACATCC AAGATAAGGA GGAAGAAACC 
CCTCTGCATT GTGCTGCTTG GCATGGTTAC TACCCAGTGG CAAAAGCTCT GTGCCAGGCT 
GGCTGCAATG TCAACATTCG CAACAGGGAA GGGGAAACAC CCATTCTGAC AGCAGCAGCA 
AGAGGGTACC ACGATATTGT GGAATGCTTG GTTGACCATG GAGGCGACCT TGATGCAACA 
GACAAGGATG GGCACATAGC ACTCCACCTT GCAGTGAGAC GGTGTCAGAT GGAAGTAGTG 
AAAACGCTCA TCATCCATGG ATGCTTTGTA GACTTCCAGG ATCGCCATGG TAACACACCT 
CTGCATGTTG CCTGCAAAGA TGGGAATCTT CCTATTGTAA TGGCACTGTG TGAGGCAAGA 
TGTAATTTGG ATATAACTAA CAAGTATGGC CGAACACCTT TACATCTGGC AGCAAACAAT 
GGTATAGTGG AAGTTGTCCG ATACCTCTGC GTCGCTGGTG CAAATGTTGA AGCCTTGACT 
TTGGATGGGA AAACAGCAGA GGATCTCGCT AGGTCAGAAC AGCATGAGTC CGTTGTCAGT 
CTGCTCGCAA GGCTTCGAAG GGATACCTAC AGAACTTTGT TCATTCAGCA GCTCAGACCT 
TCACCAACCC CCCAGCCAAG AATTAAACTG AAGTTATTTG GACACATGGG ATCAGGAAAG 
ACCACGCTTG TAGAGTCCCT CAAATGTGGC CTTATCCGAA GCTTCTTCAG AAGACGCAGA 
CCTCGTCTTT CATCAACTAA CTCTTCCCGT TTTCCTTCTT CTCCACTAAC AGCTAAGACA 
GTTTCAGTGA GCATCTCGGA TCTCTACCCT GGTTGTGAGA ATGTTAGTGT AAGAAGTCGG 
AGTATGATGT TTGAGCCAGG TCTAACCAAA GGGGTTCTTG AAGTATTTGT TTCACCGTCC 
CCTCACTGTT ACACAGATGA CCAGTCAACC AAGGCTATAG ACATCCAGAG TGCCAATCTT 
AACGGTGTTG GAGATTTTAG TGTGTGGGAA TTCTCTGGAA ACCCAACGTA TTATTGCAGC 
TATGATTATT TTGCTGCCAA TGATCCCACA TCAGTTCACA TTGTAGTGTT TAGCCTTGAA 
GAACCCTATG AGATTCAGCT GAATCAAGTG ATATTCTGGC TCAATTTCCT AAAGTCACTT 
GTTCCTGTGG AAGAACCAAT AGCGTATGGT GGGAAGCTGA AGAATCTACT CCACGTTGTG 
TTGGTGGCCA CACATGCTGA TATTGTTAAC CTTCCTCGCC CTGCCGGAGG AGAATTTGGT 
TACGACAAAA GTCTAGCATT GCTGAAAGAA GTAAGAAATA GATTTGGAAA CGATCTCCAG 
ATTTTGGATA GACTGTTTGT TCTGGATGCC GGGGGATCAG GGTCCAAGGA GATGAAGCTT 
CTGAGGAATC ACCTCCAGGA ATTAAGAAGC CAGCTGATAT CTGACTGTCC ACCTATGACA 
CCGCTTTGTG AGAAGGTGAT GTCAACTCTT CCATCCTGGA GAAAACAGAA TGGTCCCAAT 
CAACTTCTTT CTCTTCAACA ATTTGTATAT GATGTCCAGG ACCAACTAAA TCCACTAGCA 
AGTGAAGAGG ATGTTAGAAA CCTTGCATTG CAACTGCACA GTATTGGAGA AGTTAATATA 
ATGCAGAGTG AGACCGTCCA AGATGTTGTG CTTCTAGATC CACGATGGCT GTGTCACAAT 
GTCCTCGGAA AGCTACTCTC TGTAGAAAAC CCAAAAGCCC TACACCATTA CAGGGGAAGG 
TATACAATGG AAGATATTCA GCGCTTGGTC ACCGACAGTG ATGTTGATGA ACTTGTGCAG 
ATCCTGGATG CAATGGACAT TTGTGCCCGA GATCTAAGCA GCGGAGCTAT GGTGGACATC 
CCTGCCCTCA TTAAAACAGA CAACCTCCAC CGATCATGGG CTGATGAAGA AGAGGACATA 
ATGATTTATG GAGGTGTTAG AATTGTTCCT GTGGAACACC TTACTCCCTT TCCGTGTGGA 
ATTTTTCACA AAGTTCAAGT TAATTTATGC CGATGGGTAC ATCAACAGAG CGCCGACGGT 
GATGCTGATA TACGTTTATG GGTAAACGGT TGTAAAATAG CAAATCGAGG GGCAGAGGTT 
TTGGTGTTGA TGGTCAACCA TGGTCAAGGA ATTGAAGTGC AAGTTAGGGG TCTGGAAACT 
GAGAAAATCA AGTGTTGTCT ACTTCTTGAA TCCATTTGCA GTATCATTGA CAACTTATTT 
GCAACAACGT TGCCTGGTCT TTTAACTGTA AAACATTACT TGAGCCCTCA ACAGCTAAGA 
GAGCATCACG AGCCACTGAT GGTTTACCAA CCTAGAGACT TTTTTCGGGC CCAAATACAA 
AAGGAGACTT CACTGGCTAA CACCATGGCT GGATACAAAG AGAGCTTTAG CAGCATTCTT 
TGTTTTGGGT GCCTGGATGT TTACTCTCAA GGAAACCTAG GCCTGGATCA CCATGTGTCC 
GATATCAATA TTCTTGTTAG AAGAAAGCTC AGCCGACTTT TAGACCCACC AGATCCCATG 
GGAAAGGACT GGTGTCTACT AGCTATGAAT TTGGGCCTAC CTGACTTAGT TGCCAAATAC 
AATACTAACA ATTCTGTACA AAAAGACTTT TTGCCTAGTC CAATGTGTGC TCTTCTGCAG 
GAATGGAGCA ATTCTCCTGA AAGTACTGTA TCGCTCCTAA TGTCTAAACT GAGAGAACTT 
GGGCGCAGGG ATGCAGCAGA TTTTTTACTA AAAGCATCCT CTGTTTTTAA GGTGACTCTT 
GATATCCATG GACAAGAAGC CTATGCGGCA AGCTGCAACA GCGGCACATC CTATAATTCA 
ATTAGCTCAG TCGTGTCACG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dapk1 ( XM_012955236.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012810690.1 Xenopus tropicalis death-associated protein kinase 1 (dapk1), transcript variant X2, mRNA.

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CloneID	OXa31370
Clone ID Related Accession (Same CDS sequence)	XM_012955218.2 , XM_012955236.2
Accession Version	XM_012955236.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4284bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	death-associated protein kinase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012955236.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGTTT TCAGGCAAGA GAATGTGGAG GATTATTATG ATATAGGAGA GGAGCTTGGA 
AGCGGTCAGT TTGCTGTGGT GAAAAAATGC CAAGAAAAGA GCACAGGCGT GCATTATGCT 
GCAAAATTTA TCAAGAAGCG AAGGACCAAA TCAAGTCGGC GTGGGGTGAC CCGGGAGGAT 
ATAGAGAGGG AGGTCAGCAT ACTGAAGGAG ATCCAGCACC CCAATGTGAT CACGTTGCAC 
GAAGTGTACG AGAATAAAGC AGATGTCATT CTCATTCTGG AGCTAGTTGC AGGAGGTGAG 
CTATTTGACT TTCTTGCTGA AAAGGAGTCT CTGACAGAGG AGGAAGCTAC AGAGTTCATC 
AAGCAGATCC TTAATGGTGT GAATTACCTC CACTCCCTGC ACATTGCCCA CTTTGACCTC 
AAGCCTGAAA ACATCATGCT GCTTGATAGA AACGCACCAA ATCCTCGTAT TAAGATCATA 
GATTTTGGCT TGGCTCACAG AATCGACTCT GGAAATGAAT TTAAGAACAT ATTCGGAACT 
CCAGAATTTG TTGCGCCTGA AATTGTAAAT TATGAACCGC TTGGTCTTGA ATCTGATATG 
TGGAGTATTG GAGTCATAAC ATACATTCTA TTGAGCGGGG CATCTCCATT CCTTGGGGAA 
ACAAAGCAGG AGACCTTGGC TAATATATCT GCTGTCAGTT ATGACTTTGA GGAGGAGTTC 
TTTAGTAGTA CAAGTGACCT GGCCAAAGAT TTTATAAGGC GGCTGCTAGT CAAGGACCCA 
AAAAAAAGGA TGACCATTCA AGACAGTTTG TGCCACCCTT GGATTAAGCC AAAAGATACT 
CAGCAAGCAC TGAGCAGGAA AGCCTCTGCA GTCAATATGG AGAAGTTTAA GAAGTTTGCT 
GCTCGCAGAA AATGGAAACA ATCTGTCCGT TTGATATCCC TGTGCCAAAG ACTGTCAAGA 
TCCTTCTTGT CCAGGAGCAA CATGAGTATT GCTAGGAGTG ATGACACACT GGATGAGGAG 
GACTCTTTTG TAATGAAAGC CATCATTCAT GCCATAAATG ACGATAACGT TCCTGGTTTG 
CAGCATCTTT TTGGGTCTTT GTCAAACTAT GATGTGAACC AGCCAAACAA GCATGGCACC 
CCTCCCTTGT TAATTGCTGC TGGCTGTGGG AATATTCAGA TCCTTGAACT GCTCATAAAG 
CGTGGCGCAC AGATTGATGT ACAAGATAAG GCTGGGTCCA ATGCAATTTA CTGGGCTTCT 
CGTCATGGTC ACGTTGAGAC TCTGAAATTT CTACATCAGA ATAACTGTCC TTTAGATATT 
AAAGATAAGT CAGGAGAAAC AGCACTTCAC GTGGCGGCCC GTTATGGACA TGCTGAAGTT 
ATTCAATACT TGTGCAGCAT TGGATCACAT CCAGACATCC AAGATAAGGA GGAAGAAACC 
CCTCTGCATT GTGCTGCTTG GCATGGTTAC TACCCAGTGG CAAAAGCTCT GTGCCAGGCT 
GGCTGCAATG TCAACATTCG CAACAGGGAA GGGGAAACAC CCATTCTGAC AGCAGCAGCA 
AGAGGGTACC ACGATATTGT GGAATGCTTG GTTGACCATG GAGGCGACCT TGATGCAACA 
GACAAGGATG GGCACATAGC ACTCCACCTT GCAGTGAGAC GGTGTCAGAT GGAAGTAGTG 
AAAACGCTCA TCATCCATGG ATGCTTTGTA GACTTCCAGG ATCGCCATGG TAACACACCT 
CTGCATGTTG CCTGCAAAGA TGGGAATCTT CCTATTGTAA TGGCACTGTG TGAGGCAAGA 
TGTAATTTGG ATATAACTAA CAAGTATGGC CGAACACCTT TACATCTGGC AGCAAACAAT 
GGTATAGTGG AAGTTGTCCG ATACCTCTGC GTCGCTGGTG CAAATGTTGA AGCCTTGACT 
TTGGATGGGA AAACAGCAGA GGATCTCGCT AGGTCAGAAC AGCATGAGTC CGTTGTCAGT 
CTGCTCGCAA GGCTTCGAAG GGATACCTAC AGAACTTTGT TCATTCAGCA GCTCAGACCT 
TCACCAACCC CCCAGCCAAG AATTAAACTG AAGTTATTTG GACACATGGG ATCAGGAAAG 
ACCACGCTTG TAGAGTCCCT CAAATGTGGC CTTATCCGAA GCTTCTTCAG AAGACGCAGA 
CCTCGTCTTT CATCAACTAA CTCTTCCCGT TTTCCTTCTT CTCCACTAAC AGCTAAGACA 
GTTTCAGTGA GCATCTCGGA TCTCTACCCT GGTTGTGAGA ATGTTAGTGT AAGAAGTCGG 
AGTATGATGT TTGAGCCAGG TCTAACCAAA GGGGTTCTTG AAGTATTTGT TTCACCGTCC 
CCTCACTGTT ACACAGATGA CCAGTCAACC AAGGCTATAG ACATCCAGAG TGCCAATCTT 
AACGGTGTTG GAGATTTTAG TGTGTGGGAA TTCTCTGGAA ACCCAACGTA TTATTGCAGC 
TATGATTATT TTGCTGCCAA TGATCCCACA TCAGTTCACA TTGTAGTGTT TAGCCTTGAA 
GAACCCTATG AGATTCAGCT GAATCAAGTG ATATTCTGGC TCAATTTCCT AAAGTCACTT 
GTTCCTGTGG AAGAACCAAT AGCGTATGGT GGGAAGCTGA AGAATCTACT CCACGTTGTG 
TTGGTGGCCA CACATGCTGA TATTGTTAAC CTTCCTCGCC CTGCCGGAGG AGAATTTGGT 
TACGACAAAA GTCTAGCATT GCTGAAAGAA GTAAGAAATA GATTTGGAAA CGATCTCCAG 
ATTTTGGATA GACTGTTTGT TCTGGATGCC GGGGGATCAG GGTCCAAGGA GATGAAGCTT 
CTGAGGAATC ACCTCCAGGA ATTAAGAAGC CAGCTGATAT CTGACTGTCC ACCTATGACA 
CCGCTTTGTG AGAAGGTGAT GTCAACTCTT CCATCCTGGA GAAAACAGAA TGGTCCCAAT 
CAACTTCTTT CTCTTCAACA ATTTGTATAT GATGTCCAGG ACCAACTAAA TCCACTAGCA 
AGTGAAGAGG ATGTTAGAAA CCTTGCATTG CAACTGCACA GTATTGGAGA AGTTAATATA 
ATGCAGAGTG AGACCGTCCA AGATGTTGTG CTTCTAGATC CACGATGGCT GTGTCACAAT 
GTCCTCGGAA AGCTACTCTC TGTAGAAAAC CCAAAAGCCC TACACCATTA CAGGGGAAGG 
TATACAATGG AAGATATTCA GCGCTTGGTC ACCGACAGTG ATGTTGATGA ACTTGTGCAG 
ATCCTGGATG CAATGGACAT TTGTGCCCGA GATCTAAGCA GCGGAGCTAT GGTGGACATC 
CCTGCCCTCA TTAAAACAGA CAACCTCCAC CGATCATGGG CTGATGAAGA AGAGGACATA 
ATGATTTATG GAGGTGTTAG AATTGTTCCT GTGGAACACC TTACTCCCTT TCCGTGTGGA 
ATTTTTCACA AAGTTCAAGT TAATTTATGC CGATGGGTAC ATCAACAGAG CGCCGACGGT 
GATGCTGATA TACGTTTATG GGTAAACGGT TGTAAAATAG CAAATCGAGG GGCAGAGGTT 
TTGGTGTTGA TGGTCAACCA TGGTCAAGGA ATTGAAGTGC AAGTTAGGGG TCTGGAAACT 
GAGAAAATCA AGTGTTGTCT ACTTCTTGAA TCCATTTGCA GTATCATTGA CAACTTATTT 
GCAACAACGT TGCCTGGTCT TTTAACTGTA AAACATTACT TGAGCCCTCA ACAGCTAAGA 
GAGCATCACG AGCCACTGAT GGTTTACCAA CCTAGAGACT TTTTTCGGGC CCAAATACAA 
AAGGAGACTT CACTGGCTAA CACCATGGCT GGATACAAAG AGAGCTTTAG CAGCATTCTT 
TGTTTTGGGT GCCTGGATGT TTACTCTCAA GGAAACCTAG GCCTGGATCA CCATGTGTCC 
GATATCAATA TTCTTGTTAG AAGAAAGCTC AGCCGACTTT TAGACCCACC AGATCCCATG 
GGAAAGGACT GGTGTCTACT AGCTATGAAT TTGGGCCTAC CTGACTTAGT TGCCAAATAC 
AATACTAACA ATTCTGTACA AAAAGACTTT TTGCCTAGTC CAATGTGTGC TCTTCTGCAG 
GAATGGAGCA ATTCTCCTGA AAGTACTGTA TCGCTCCTAA TGTCTAAACT GAGAGAACTT 
GGGCGCAGGG ATGCAGCAGA TTTTTTACTA AAAGCATCCT CTGTTTTTAA GGTGACTCTT 
GATATCCATG GACAAGAAGC CTATGCGGCA AGCTGCAACA GCGGCACATC CTATAATTCA 
ATTAGCTCAG TCGTGTCACG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012810690.1
CDS	979..5262
Translation	MTVFRQENVEDYYDIGEELGSGQFAVVKKCQEKSTGVHYAAKFIKKRRTKSSRRGVTREDIEREVSILKEIQHPNVITLHEVYENKADVILILELVAGGELFDFLAEKESLTEEEATEFIKQILNGVNYLHSLHIAHFDLKPENIMLLDRNAPNPRIKIIDFGLAHRIDSGNEFKNIFGTPEFVAPEIVNYEPLGLESDMWSIGVITYILLSGASPFLGETKQETLANISAVSYDFEEEFFSSTSDLAKDFIRRLLVKDPKKRMTIQDSLCHPWIKPKDTQQALSRKASAVNMEKFKKFAARRKWKQSVRLISLCQRLSRSFLSRSNMSIARSDDTLDEEDSFVMKAIIHAINDDNVPGLQHLFGSLSNYDVNQPNKHGTPPLLIAAGCGNIQILELLIKRGAQIDVQDKAGSNAIYWASRHGHVETLKFLHQNNCPLDIKDKSGETALHVAARYGHAEVIQYLCSIGSHPDIQDKEEETPLHCAAWHGYYPVAKALCQAGCNVNIRNREGETPILTAAARGYHDIVECLVDHGGDLDATDKDGHIALHLAVRRCQMEVVKTLIIHGCFVDFQDRHGNTPLHVACKDGNLPIVMALCEARCNLDITNKYGRTPLHLAANNGIVEVVRYLCVAGANVEALTLDGKTAEDLARSEQHESVVSLLARLRRDTYRTLFIQQLRPSPTPQPRIKLKLFGHMGSGKTTLVESLKCGLIRSFFRRRRPRLSSTNSSRFPSSPLTAKTVSVSISDLYPGCENVSVRSRSMMFEPGLTKGVLEVFVSPSPHCYTDDQSTKAIDIQSANLNGVGDFSVWEFSGNPTYYCSYDYFAANDPTSVHIVVFSLEEPYEIQLNQVIFWLNFLKSLVPVEEPIAYGGKLKNLLHVVLVATHADIVNLPRPAGGEFGYDKSLALLKEVRNRFGNDLQILDRLFVLDAGGSGSKEMKLLRNHLQELRSQLISDCPPMTPLCEKVMSTLPSWRKQNGPNQLLSLQQFVYDVQDQLNPLASEEDVRNLALQLHSIGEVNIMQSETVQDVVLLDPRWLCHNVLGKLLSVENPKALHHYRGRYTMEDIQRLVTDSDVDELVQILDAMDICARDLSSGAMVDIPALIKTDNLHRSWADEEEDIMIYGGVRIVPVEHLTPFPCGIFHKVQVNLCRWVHQQSADGDADIRLWVNGCKIANRGAEVLVLMVNHGQGIEVQVRGLETEKIKCCLLLESICSIIDNLFATTLPGLLTVKHYLSPQQLREHHEPLMVYQPRDFFRAQIQKETSLANTMAGYKESFSSILCFGCLDVYSQGNLGLDHHVSDINILVRRKLSRLLDPPDPMGKDWCLLAMNLGLPDLVAKYNTNNSVQKDFLPSPMCALLQEWSNSPESTVSLLMSKLRELGRRDAADFLLKASSVFKVTLDIHGQEAYAASCNSGTSYNSISSVVSR

Target ORF information:

RefSeq Version	XM_012955236.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis death-associated protein kinase 1 (dapk1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012955236.2

ORF Insert Sequence:

ATGACTGTTT TCAGGCAAGA GAATGTGGAG GATTATTATG ATATAGGAGA GGAGCTTGGA 
AGCGGTCAGT TTGCTGTGGT GAAAAAATGC CAAGAAAAGA GCACAGGCGT GCATTATGCT 
GCAAAATTTA TCAAGAAGCG AAGGACCAAA TCAAGTCGGC GTGGGGTGAC CCGGGAGGAT 
ATAGAGAGGG AGGTCAGCAT ACTGAAGGAG ATCCAGCACC CCAATGTGAT CACGTTGCAC 
GAAGTGTACG AGAATAAAGC AGATGTCATT CTCATTCTGG AGCTAGTTGC AGGAGGTGAG 
CTATTTGACT TTCTTGCTGA AAAGGAGTCT CTGACAGAGG AGGAAGCTAC AGAGTTCATC 
AAGCAGATCC TTAATGGTGT GAATTACCTC CACTCCCTGC ACATTGCCCA CTTTGACCTC 
AAGCCTGAAA ACATCATGCT GCTTGATAGA AACGCACCAA ATCCTCGTAT TAAGATCATA 
GATTTTGGCT TGGCTCACAG AATCGACTCT GGAAATGAAT TTAAGAACAT ATTCGGAACT 
CCAGAATTTG TTGCGCCTGA AATTGTAAAT TATGAACCGC TTGGTCTTGA ATCTGATATG 
TGGAGTATTG GAGTCATAAC ATACATTCTA TTGAGCGGGG CATCTCCATT CCTTGGGGAA 
ACAAAGCAGG AGACCTTGGC TAATATATCT GCTGTCAGTT ATGACTTTGA GGAGGAGTTC 
TTTAGTAGTA CAAGTGACCT GGCCAAAGAT TTTATAAGGC GGCTGCTAGT CAAGGACCCA 
AAAAAAAGGA TGACCATTCA AGACAGTTTG TGCCACCCTT GGATTAAGCC AAAAGATACT 
CAGCAAGCAC TGAGCAGGAA AGCCTCTGCA GTCAATATGG AGAAGTTTAA GAAGTTTGCT 
GCTCGCAGAA AATGGAAACA ATCTGTCCGT TTGATATCCC TGTGCCAAAG ACTGTCAAGA 
TCCTTCTTGT CCAGGAGCAA CATGAGTATT GCTAGGAGTG ATGACACACT GGATGAGGAG 
GACTCTTTTG TAATGAAAGC CATCATTCAT GCCATAAATG ACGATAACGT TCCTGGTTTG 
CAGCATCTTT TTGGGTCTTT GTCAAACTAT GATGTGAACC AGCCAAACAA GCATGGCACC 
CCTCCCTTGT TAATTGCTGC TGGCTGTGGG AATATTCAGA TCCTTGAACT GCTCATAAAG 
CGTGGCGCAC AGATTGATGT ACAAGATAAG GCTGGGTCCA ATGCAATTTA CTGGGCTTCT 
CGTCATGGTC ACGTTGAGAC TCTGAAATTT CTACATCAGA ATAACTGTCC TTTAGATATT 
AAAGATAAGT CAGGAGAAAC AGCACTTCAC GTGGCGGCCC GTTATGGACA TGCTGAAGTT 
ATTCAATACT TGTGCAGCAT TGGATCACAT CCAGACATCC AAGATAAGGA GGAAGAAACC 
CCTCTGCATT GTGCTGCTTG GCATGGTTAC TACCCAGTGG CAAAAGCTCT GTGCCAGGCT 
GGCTGCAATG TCAACATTCG CAACAGGGAA GGGGAAACAC CCATTCTGAC AGCAGCAGCA 
AGAGGGTACC ACGATATTGT GGAATGCTTG GTTGACCATG GAGGCGACCT TGATGCAACA 
GACAAGGATG GGCACATAGC ACTCCACCTT GCAGTGAGAC GGTGTCAGAT GGAAGTAGTG 
AAAACGCTCA TCATCCATGG ATGCTTTGTA GACTTCCAGG ATCGCCATGG TAACACACCT 
CTGCATGTTG CCTGCAAAGA TGGGAATCTT CCTATTGTAA TGGCACTGTG TGAGGCAAGA 
TGTAATTTGG ATATAACTAA CAAGTATGGC CGAACACCTT TACATCTGGC AGCAAACAAT 
GGTATAGTGG AAGTTGTCCG ATACCTCTGC GTCGCTGGTG CAAATGTTGA AGCCTTGACT 
TTGGATGGGA AAACAGCAGA GGATCTCGCT AGGTCAGAAC AGCATGAGTC CGTTGTCAGT 
CTGCTCGCAA GGCTTCGAAG GGATACCTAC AGAACTTTGT TCATTCAGCA GCTCAGACCT 
TCACCAACCC CCCAGCCAAG AATTAAACTG AAGTTATTTG GACACATGGG ATCAGGAAAG 
ACCACGCTTG TAGAGTCCCT CAAATGTGGC CTTATCCGAA GCTTCTTCAG AAGACGCAGA 
CCTCGTCTTT CATCAACTAA CTCTTCCCGT TTTCCTTCTT CTCCACTAAC AGCTAAGACA 
GTTTCAGTGA GCATCTCGGA TCTCTACCCT GGTTGTGAGA ATGTTAGTGT AAGAAGTCGG 
AGTATGATGT TTGAGCCAGG TCTAACCAAA GGGGTTCTTG AAGTATTTGT TTCACCGTCC 
CCTCACTGTT ACACAGATGA CCAGTCAACC AAGGCTATAG ACATCCAGAG TGCCAATCTT 
AACGGTGTTG GAGATTTTAG TGTGTGGGAA TTCTCTGGAA ACCCAACGTA TTATTGCAGC 
TATGATTATT TTGCTGCCAA TGATCCCACA TCAGTTCACA TTGTAGTGTT TAGCCTTGAA 
GAACCCTATG AGATTCAGCT GAATCAAGTG ATATTCTGGC TCAATTTCCT AAAGTCACTT 
GTTCCTGTGG AAGAACCAAT AGCGTATGGT GGGAAGCTGA AGAATCTACT CCACGTTGTG 
TTGGTGGCCA CACATGCTGA TATTGTTAAC CTTCCTCGCC CTGCCGGAGG AGAATTTGGT 
TACGACAAAA GTCTAGCATT GCTGAAAGAA GTAAGAAATA GATTTGGAAA CGATCTCCAG 
ATTTTGGATA GACTGTTTGT TCTGGATGCC GGGGGATCAG GGTCCAAGGA GATGAAGCTT 
CTGAGGAATC ACCTCCAGGA ATTAAGAAGC CAGCTGATAT CTGACTGTCC ACCTATGACA 
CCGCTTTGTG AGAAGGTGAT GTCAACTCTT CCATCCTGGA GAAAACAGAA TGGTCCCAAT 
CAACTTCTTT CTCTTCAACA ATTTGTATAT GATGTCCAGG ACCAACTAAA TCCACTAGCA 
AGTGAAGAGG ATGTTAGAAA CCTTGCATTG CAACTGCACA GTATTGGAGA AGTTAATATA 
ATGCAGAGTG AGACCGTCCA AGATGTTGTG CTTCTAGATC CACGATGGCT GTGTCACAAT 
GTCCTCGGAA AGCTACTCTC TGTAGAAAAC CCAAAAGCCC TACACCATTA CAGGGGAAGG 
TATACAATGG AAGATATTCA GCGCTTGGTC ACCGACAGTG ATGTTGATGA ACTTGTGCAG 
ATCCTGGATG CAATGGACAT TTGTGCCCGA GATCTAAGCA GCGGAGCTAT GGTGGACATC 
CCTGCCCTCA TTAAAACAGA CAACCTCCAC CGATCATGGG CTGATGAAGA AGAGGACATA 
ATGATTTATG GAGGTGTTAG AATTGTTCCT GTGGAACACC TTACTCCCTT TCCGTGTGGA 
ATTTTTCACA AAGTTCAAGT TAATTTATGC CGATGGGTAC ATCAACAGAG CGCCGACGGT 
GATGCTGATA TACGTTTATG GGTAAACGGT TGTAAAATAG CAAATCGAGG GGCAGAGGTT 
TTGGTGTTGA TGGTCAACCA TGGTCAAGGA ATTGAAGTGC AAGTTAGGGG TCTGGAAACT 
GAGAAAATCA AGTGTTGTCT ACTTCTTGAA TCCATTTGCA GTATCATTGA CAACTTATTT 
GCAACAACGT TGCCTGGTCT TTTAACTGTA AAACATTACT TGAGCCCTCA ACAGCTAAGA 
GAGCATCACG AGCCACTGAT GGTTTACCAA CCTAGAGACT TTTTTCGGGC CCAAATACAA 
AAGGAGACTT CACTGGCTAA CACCATGGCT GGATACAAAG AGAGCTTTAG CAGCATTCTT 
TGTTTTGGGT GCCTGGATGT TTACTCTCAA GGAAACCTAG GCCTGGATCA CCATGTGTCC 
GATATCAATA TTCTTGTTAG AAGAAAGCTC AGCCGACTTT TAGACCCACC AGATCCCATG 
GGAAAGGACT GGTGTCTACT AGCTATGAAT TTGGGCCTAC CTGACTTAGT TGCCAAATAC 
AATACTAACA ATTCTGTACA AAAAGACTTT TTGCCTAGTC CAATGTGTGC TCTTCTGCAG 
GAATGGAGCA ATTCTCCTGA AAGTACTGTA TCGCTCCTAA TGTCTAAACT GAGAGAACTT 
GGGCGCAGGG ATGCAGCAGA TTTTTTACTA AAAGCATCCT CTGTTTTTAA GGTGACTCTT 
GATATCCATG GACAAGAAGC CTATGCGGCA AGCTGCAACA GCGGCACATC CTATAATTCA 
ATTAGCTCAG TCGTGTCACG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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dapk1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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