usp32 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	usp32
Entrez Gene ID	557981
Full Name	ubiquitin specific peptidase 32
Synonyms	wu:fe14d02
General protein information	Preferred Names ubiquitin specific peptidase 32 Names ubiquitin carboxyl-terminal hydrolase 32
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_681144.8	XP_686236.6	ubiquitin carboxyl-terminal hydrolase 32 isoform X1
XM_005157606.3	XP_005157663.1	ubiquitin carboxyl-terminal hydrolase 32 isoform X2
XM_009291747.2	XP_009290022.1	ubiquitin carboxyl-terminal hydrolase 32 isoform X3
XM_005157606.4	XP_005157663.1	ubiquitin carboxyl-terminal hydrolase 32 isoform X2
XM_009291747.3	XP_009290022.1	ubiquitin carboxyl-terminal hydrolase 32 isoform X3
XM_681144.9	XP_686236.6	ubiquitin carboxyl-terminal hydrolase 32 isoform X1

Pathways from BioSystems

Homologies

	LOC716857	XP_002808172.1
Canis lupus familiaris (dog)	USP32	XP_005624819.1
Bos taurus (cattle)	USP32	XP_002695689.2
Gallus gallus (chicken)	USP32	XP_004946797.1
Drosophila melanogaster (fruit fly)	CG8334	NP_001262075.1
Caenorhabditis elegans (roundworm)	cyk-3	NP_001022992.1
Xenopus tropicalis (tropical clawed frog)	usp32	XP_002933869.1
Mus musculus (house mouse)	Usp32	NP_001025105.1
Rattus norvegicus (Norway rat)	Usp32	NP_001100502.1
Danio rerio (zebrafish)	usp32	XP_686236.6
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001209	XP_321947.5
Homo sapiens (human)	USP32	NP_115971.2

Gene Ontology

The following usp32 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the usp32 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa20507	XM_681144.9 Latest version!	Danio rerio ubiquitin specific peptidase 32 (usp32), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa20508	XM_005157606.4 Latest version!	Danio rerio ubiquitin specific peptidase 32 (usp32), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa42710	XM_009291747.3 Latest version!	Danio rerio ubiquitin specific peptidase 32 (usp32), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
View Old Accession Versions >>
ODa20508	XM_005157606.3	Danio rerio ubiquitin specific peptidase 32 (usp32), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa42710	XM_009291747.2	Danio rerio ubiquitin specific peptidase 32 (usp32), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa20507	XM_681144.8	Danio rerio ubiquitin specific peptidase 32 (usp32), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

usp32 ( XM_681144.8 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_686236.6 Danio rerio ubiquitin specific peptidase 32 (usp32), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa20507
Clone ID Related Accession (Same CDS sequence)	XM_681144.8 , XM_681144.9
Accession Version	XM_681144.8	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4758bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ubiquitin carboxyl-terminal hydrolase 32 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_681144.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGCCA AAGAGTCACG GATAGGCTTC CTTTCGTATG ACGAGGCCGT TAAAAGAGTT 
ACCGATGTGG AGCTCAAACG TCTGCGAGAT GCCTTTAAGA GGACCAGTGG CCTGTCAGTG 
TATATGACCC AGCAATGCTT CTTCAGAGAA GTCCTGGGCG ACGGAGTGCC ACCAAAGGTT 
GCAGAAGTGA TCTACAACTC ATTTGGTGGC ACATCCAAAG GGCTACACTT CAACTATCTG 
ATCGTCGGCC TGGTTTTGTT AACAAGAGGG CGTGATGAAG AGAAAGCCAA ATACATCTTC 
AGCTTGTTCG CGAGTGATTC TGGCACCTTT GTGGTCCGGG AGGATGTGGA GAACATGCTG 
CGCTCAGTGG ATGGGGAGGT CCCGCCTTCT CTCAGGAAGT GCTTTACTGA GGGGGAGAAG 
GTGAATTATG AGAAGTTTAA GAGCTGGCTG CTTCAGAATA AAGATGCTTT CACGTTCTCC 
CGCTGGCTGC TGTCAGGTGG TGTGTGCGTA ACGTTGACTG ACGACAGCGA CACACCCACC 
TTCTATCAAA CCCTGGCAGG TGTCACTCAC TTAGAAGAAT CAGACATTAT CGACTTGGAA 
AAACGCTACT GGCTGCTGAA AGCTCAATCA AGGACAGGCC GCTTTGATTT GGAAACCTTT 
GTCCCGCTGG TCTCGCCCCC TATTCATGCA TCGCTGAGTG AAGGCTTGTT TCACGCCTTT 
GATGAGAATC GGGACAATCA TATTGACTTT AAGGAGATAT CCTGTGGGCT CTCAGCTTGC 
TGTCGCGGCC CAGTAGCGGA GAGGCAGAAG TTTTGTTTTA AAGTGTTCGA CGTAGATCGT 
GATGGAGTTT TGTCCAGGGA CGAGATCCAC GAGATGGTGG TGGCGCTGCT GGAGGTGTGG 
AAGGATAACC GAACAGACAC TCTTCCTGAG TTTGATTCCA GTGTGTCAGA GATTGTGGAG 
GACATATTGA AAATGCACGA CACAACAAAA CAAGGACATT TAACTCTAGA GGACTATCAG 
ATATGGAGTG TGAAAAGTGC CCTCGCCAAT GAGTTCCTCA ACTTGCTGTT TCAGGTGTGT 
CATATTGTCC TGGGCCTGCG TCCTGCCACC CCTGAAGAGG AAGGCCAGAT CATTAGGGGT 
TGGTTGGAGA GGGAAAGCAG GCACGGTTTA CAGCCCAGTC AAAACTGGTT CCTGATCTCC 
ATGCAGTGGT GGCAGCAATG GAAAGACTAT GTCAGATATG ATAATAAGTC GATTGTCATG 
GAGCAGCCAT CAGTGCAGGG CAGCGGGATG AGAAGTTCTC AGTCTGCTGC ATCTGTAGAG 
CCTATCCTCA ACAGAATTGG ATTAGTGGTT CGCTCCCCCA GTTCCCCAGA AGAAAAATTC 
CCTGACAACA TCTCCAGCTC ATCAGAGGCT TCAGAGAGCA CTGGTAATAG CCTAACCCCA 
CACATCAGCT CCTCTGCCAC AGACGTGTGC TTTGCTCGGC AGCACAACAT CTCTGACAAC 
AACAACCAGT GTTTCTCAAG CGCCAATGGC CATTTGCCTA ACAGCTTGGC CGTGCAGAGA 
CCCGGTGCGA TAGACAACCA GCCTCTGGTG ACCACCGAGC CCATGAAGGC ACCCACGCTG 
ACTATGGAAG GAGGTCGACT GAAGAAGTCC CATCAACTGT TGTCTGGCCG GGAGTTTGAG 
ACGGTTCCAG AGCCGGTGTG GAGAGCACTT TACCACTGGT ATGGGGCCAA CCTTAGTCTG 
CCCCGACCGG TTATCAAGTC TACAGTCAAG GCACAGCCTG AGCTAGAGCT CTTCCCCCTT 
TACCTTCTGT TCCTGCGACA GCAACCGGCC ACACGGAGCC CTCAGTCCAA CATCTGGGTC 
AATATGGGTA ATGTTCCTTC TCCCAACGCC CCGCTCAAGC GAGTGCTGGC ATACACAGGC 
TGTTTCAGCA GAATGTGCAC CATTAAAGAC ATTCACGAGT ACCTGTCCCA GAGGTTACGC 
ATCAAAGAAG AGGACATGCG CCTCTGGCTT TACAACAGCG AGAATTACCT CACTCTCCTG 
GACGATGAAG ATCACACTTT AGAAAGCTTG AAAATTCCAG ATGAACAGCA CTTAGTCATT 
GAGGTTCGAA ACAAAGATAT GAGCTGGCCA GAGGAAATGT CTTTCATTGC AAACAGCAGC 
AAAATCGACA GACACAAAGT TCCTACTGAG AAAGGTGCCA CAGGTTTGAG TAATCTGGGC 
AACACGTGCT TCATGAACTC CAGTATTCAG TGTGTAAGCA ATACCAAGCC CCTCACTGAG 
TACTTCATCT CAGGGCGACA CCTTTATGAG CTTAATAGAA CTAATCCAAT AGGAATGCGT 
GGTCACATGG CCAAATGCTA TGGTGATTTG GTACAGGAAC TATGGAGCGG CACTCAAAAG 
AATGTTGCGC CTTTGAAACT TAGGTGGACG ATAGCAAAGT ACGCGCCAAG ATTCAATGGC 
TTCCAGCAGC AAGACTCCCA GGAGCTTTTG GCCTTCCTGT TGGATGGGCT TCATGAAGAC 
TTGAATCGTG TGCATGAGAA GCCGTATGTG GAGCTCAAGG ACAGTGATGG ACGGCCGGAC 
CATGAGGTTG CTACAGAGGC CTGGGAAAAC CATTTGCGGA GAAATCGCTC TATCGTGGTG 
GACCTCTTCC ATGGGCAGCT CAGATCCCAG GTGAAGTGCA AGACCTGTGG CCACATAAGC 
GCACGCTTCG ACCCCTTTAA CTTCCTATCG CTGCCTCTGC CAATGGACAG CTCCATGCAC 
TTAGAAATCA CAGTCATCAA ACTTGACGGC TCCACTCCAG TGCGATACGG GCTCCGTCTC 
AACATGGATG AGAAGTACAT GGGGCTAAAA AAACAGCTGA GCGAGCTCTG TAGCCTGAAA 
CCTGAGCAGA TCCTACTGGC CGAAGTTCAC ACGTCCAACA TCAAGAACTT TCCTCTGGAT 
AACCAGAAGG TGCGACACTC TGTCAACGGC TTCCTCTGTG CGTTTGAGAT CCCTGTGCCT 
GGTTCACCCA CTTCAGTCAC TTCTCCTGTT CAAACAGAAA AACCACCTAT TGCCAATGGG 
ACAGCAGGAA ACAATCATAT TGGAAAGTCT GGCCTGATTC CTAATGGTAT GCCCAGTACA 
GTGGTCCCCT GCACCCTTGA GAAACCCCTC GCCAATGGTA TCCCCAATGG CCATGCTGTG 
CCAGTGCAGG ACAGCCCATT CATTGGCTAC ATTATTGCCA TGCACAGAAA GATGATGCGG 
ACGGAACTGT ACTTCCTGTC CTCTCAGAAG AACAGGCCCA GTCTGTTTGG TATGCCTCTG 
ATTGTGCCAT GCACAGTGCA CACGAGTAAG AAGGACCTGT ATGATGCTGT GTGGATTCAA 
GTGTCCCGAC TTGCAAGTCC ACTGCCGCCG CAGGAAGCCA GCAACCATGC TCAAGACTGT 
GATGACAGTT TGGGCTATCA GTACCCCTTT ACGCTGCGGG TGGTGCAGAA AGATGGTAAC 
TCCTGTGCCT GGTGTCCCTG GTACAGGTTC TGCAGAGGCT GCACAGTGGA CTGTAATGAA 
GACAGAGCAT CTTTGGGGAA TGCCTACATT GCTGTAGACT GGGATCCCAC CGCCTTGCAC 
CTTCGTTACC AGACGTCTCA GGAAAGGATA GTTGAAGAGC ATCCCAGTGT TGAGCAGAGT 
CGACGCGCTC AAGCAGAGCC CATCAGTCTG GACAGCTGTC TGAAAGCCTT CACCAGTGAA 
GAAGAGCTGG GAGAGGACGA GCTCTATTAC TGCTCCAAGT GTAAAACACA CCGCCTGGCC 
ACTAAGAAGC TGGACCTCTG GAGGCTGCCT CCTGTACTGA TTGTTCATTT GAAACGCTTC 
CAATTTGTAA ATGGTCGATG GATCAAGTCT CAGAAGATTG TGAAGTTTCC ACGCGAGAGC 
TTCGACCCCA GTGCGTTCCT TGCCCCACGG GAGGTGTCTC AGAGCCTTGG CAGCCTGCAG 
AGCCTGCAGA GCGAGTGCCT GGTGGAGGAG CTCCAGAGGC TGGAGAGAGG AGACTCCTCT 
GTGTCCTCCA TCTCCACCAC CACCTTGGCC AGTCCTCCCA TATCTGGCAG AGCCACCCCG 
TGCTCCATCA GGAGGGTCGG CAGTCCCCTG AGCAGAAACA GCAGTCCCGG CAGCAGCCCC 
AGAAACACAG GCCGAAAACA GGGCCGCTTG CGTCTTCCCC AGCTGGGCAG CAGACATCGA 
CTCTGCAACA GCAAAGAAAA CCTGGAAGCC TGTAGCACCA ACAAGGAGAG CGAGAGCGAG 
GCCGAGCAAA GCGCAGTGAC GGACGGGGAG AATCTGACCC CTGATGCCAA CACACAAGAC 
ACCTGGTCAC TAGACGCCTC CAGCAACGAC TGCGATGTCA TTATGGTGAA TGGACTAGGA 
CAGGACAGCA GTCAGAGCAA TGGACATTCA GAGGTCAGCG CCGATCTGGA CACCCCTCAC 
CAAAGAAATG ACATCTGCCT GGAGTCCCTG TATAATTTAT ATGCAATCTC ATGCCATTCG 
GGAATAATGG GAGGGGGGCA CTATGTGACT TATGCCAAAA ACCCCAACGA AAAATGGTAC 
TGTTACAATG ACAGCAGCTG CAAGGAAGTG CACTCGGACG AGATTGACAC AGATTCAGCC 
TACATCCTGT TTTATGAGCA ACAGGGAGTG GACTACTCGC AGTTTCTGCC AAAAACGGAG 
GGCAAGAAGA TGGCAGACAC CACTAGCATG GATGAAGACT TTGAATCTGA CTACAAGAAG 
TACTGTGTGC TGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_686236.6
CDS	160..4917
Translation	MGAKESRIGFLSYDEAVKRVTDVELKRLRDAFKRTSGLSVYMTQQCFFREVLGDGVPPKVAEVIYNSFGGTSKGLHFNYLIVGLVLLTRGRDEEKAKYIFSLFASDSGTFVVREDVENMLRSVDGEVPPSLRKCFTEGEKVNYEKFKSWLLQNKDAFTFSRWLLSGGVCVTLTDDSDTPTFYQTLAGVTHLEESDIIDLEKRYWLLKAQSRTGRFDLETFVPLVSPPIHASLSEGLFHAFDENRDNHIDFKEISCGLSACCRGPVAERQKFCFKVFDVDRDGVLSRDEIHEMVVALLEVWKDNRTDTLPEFDSSVSEIVEDILKMHDTTKQGHLTLEDYQIWSVKSALANEFLNLLFQVCHIVLGLRPATPEEEGQIIRGWLERESRHGLQPSQNWFLISMQWWQQWKDYVRYDNKSIVMEQPSVQGSGMRSSQSAASVEPILNRIGLVVRSPSSPEEKFPDNISSSSEASESTGNSLTPHISSSATDVCFARQHNISDNNNQCFSSANGHLPNSLAVQRPGAIDNQPLVTTEPMKAPTLTMEGGRLKKSHQLLSGREFETVPEPVWRALYHWYGANLSLPRPVIKSTVKAQPELELFPLYLLFLRQQPATRSPQSNIWVNMGNVPSPNAPLKRVLAYTGCFSRMCTIKDIHEYLSQRLRIKEEDMRLWLYNSENYLTLLDDEDHTLESLKIPDEQHLVIEVRNKDMSWPEEMSFIANSSKIDRHKVPTEKGATGLSNLGNTCFMNSSIQCVSNTKPLTEYFISGRHLYELNRTNPIGMRGHMAKCYGDLVQELWSGTQKNVAPLKLRWTIAKYAPRFNGFQQQDSQELLAFLLDGLHEDLNRVHEKPYVELKDSDGRPDHEVATEAWENHLRRNRSIVVDLFHGQLRSQVKCKTCGHISARFDPFNFLSLPLPMDSSMHLEITVIKLDGSTPVRYGLRLNMDEKYMGLKKQLSELCSLKPEQILLAEVHTSNIKNFPLDNQKVRHSVNGFLCAFEIPVPGSPTSVTSPVQTEKPPIANGTAGNNHIGKSGLIPNGMPSTVVPCTLEKPLANGIPNGHAVPVQDSPFIGYIIAMHRKMMRTELYFLSSQKNRPSLFGMPLIVPCTVHTSKKDLYDAVWIQVSRLASPLPPQEASNHAQDCDDSLGYQYPFTLRVVQKDGNSCAWCPWYRFCRGCTVDCNEDRASLGNAYIAVDWDPTALHLRYQTSQERIVEEHPSVEQSRRAQAEPISLDSCLKAFTSEEELGEDELYYCSKCKTHRLATKKLDLWRLPPVLIVHLKRFQFVNGRWIKSQKIVKFPRESFDPSAFLAPREVSQSLGSLQSLQSECLVEELQRLERGDSSVSSISTTTLASPPISGRATPCSIRRVGSPLSRNSSPGSSPRNTGRKQGRLRLPQLGSRHRLCNSKENLEACSTNKESESEAEQSAVTDGENLTPDANTQDTWSLDASSNDCDVIMVNGLGQDSSQSNGHSEVSADLDTPHQRNDICLESLYNLYAISCHSGIMGGGHYVTYAKNPNEKWYCYNDSSCKEVHSDEIDTDSAYILFYEQQGVDYSQFLPKTEGKKMADTTSMDEDFESDYKKYCVLQ

Target ORF information:

RefSeq Version	XM_681144.8
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ubiquitin specific peptidase 32 (usp32), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_681144.8

ORF Insert Sequence:

ATGGGCGCCA AAGAGTCACG GATAGGCTTC CTTTCGTATG ACGAGGCCGT TAAAAGAGTT 
ACCGATGTGG AGCTCAAACG TCTGCGAGAT GCCTTTAAGA GGACCAGTGG CCTGTCAGTG 
TATATGACCC AGCAATGCTT CTTCAGAGAA GTCCTGGGCG ACGGAGTGCC ACCAAAGGTT 
GCAGAAGTGA TCTACAACTC ATTTGGTGGC ACATCCAAAG GGCTACACTT CAACTATCTG 
ATCGTCGGCC TGGTTTTGTT AACAAGAGGG CGTGATGAAG AGAAAGCCAA ATACATCTTC 
AGCTTGTTCG CGAGTGATTC TGGCACCTTT GTGGTCCGGG AGGATGTGGA GAACATGCTG 
CGCTCAGTGG ATGGGGAGGT CCCGCCTTCT CTCAGGAAGT GCTTTACTGA GGGGGAGAAG 
GTGAATTATG AGAAGTTTAA GAGCTGGCTG CTTCAGAATA AAGATGCTTT CACGTTCTCC 
CGCTGGCTGC TGTCAGGTGG TGTGTGCGTA ACGTTGACTG ACGACAGCGA CACACCCACC 
TTCTATCAAA CCCTGGCAGG TGTCACTCAC TTAGAAGAAT CAGACATTAT CGACTTGGAA 
AAACGCTACT GGCTGCTGAA AGCTCAATCA AGGACAGGCC GCTTTGATTT GGAAACCTTT 
GTCCCGCTGG TCTCGCCCCC TATTCATGCA TCGCTGAGTG AAGGCTTGTT TCACGCCTTT 
GATGAGAATC GGGACAATCA TATTGACTTT AAGGAGATAT CCTGTGGGCT CTCAGCTTGC 
TGTCGCGGCC CAGTAGCGGA GAGGCAGAAG TTTTGTTTTA AAGTGTTCGA CGTAGATCGT 
GATGGAGTTT TGTCCAGGGA CGAGATCCAC GAGATGGTGG TGGCGCTGCT GGAGGTGTGG 
AAGGATAACC GAACAGACAC TCTTCCTGAG TTTGATTCCA GTGTGTCAGA GATTGTGGAG 
GACATATTGA AAATGCACGA CACAACAAAA CAAGGACATT TAACTCTAGA GGACTATCAG 
ATATGGAGTG TGAAAAGTGC CCTCGCCAAT GAGTTCCTCA ACTTGCTGTT TCAGGTGTGT 
CATATTGTCC TGGGCCTGCG TCCTGCCACC CCTGAAGAGG AAGGCCAGAT CATTAGGGGT 
TGGTTGGAGA GGGAAAGCAG GCACGGTTTA CAGCCCAGTC AAAACTGGTT CCTGATCTCC 
ATGCAGTGGT GGCAGCAATG GAAAGACTAT GTCAGATATG ATAATAAGTC GATTGTCATG 
GAGCAGCCAT CAGTGCAGGG CAGCGGGATG AGAAGTTCTC AGTCTGCTGC ATCTGTAGAG 
CCTATCCTCA ACAGAATTGG ATTAGTGGTT CGCTCCCCCA GTTCCCCAGA AGAAAAATTC 
CCTGACAACA TCTCCAGCTC ATCAGAGGCT TCAGAGAGCA CTGGTAATAG CCTAACCCCA 
CACATCAGCT CCTCTGCCAC AGACGTGTGC TTTGCTCGGC AGCACAACAT CTCTGACAAC 
AACAACCAGT GTTTCTCAAG CGCCAATGGC CATTTGCCTA ACAGCTTGGC CGTGCAGAGA 
CCCGGTGCGA TAGACAACCA GCCTCTGGTG ACCACCGAGC CCATGAAGGC ACCCACGCTG 
ACTATGGAAG GAGGTCGACT GAAGAAGTCC CATCAACTGT TGTCTGGCCG GGAGTTTGAG 
ACGGTTCCAG AGCCGGTGTG GAGAGCACTT TACCACTGGT ATGGGGCCAA CCTTAGTCTG 
CCCCGACCGG TTATCAAGTC TACAGTCAAG GCACAGCCTG AGCTAGAGCT CTTCCCCCTT 
TACCTTCTGT TCCTGCGACA GCAACCGGCC ACACGGAGCC CTCAGTCCAA CATCTGGGTC 
AATATGGGTA ATGTTCCTTC TCCCAACGCC CCGCTCAAGC GAGTGCTGGC ATACACAGGC 
TGTTTCAGCA GAATGTGCAC CATTAAAGAC ATTCACGAGT ACCTGTCCCA GAGGTTACGC 
ATCAAAGAAG AGGACATGCG CCTCTGGCTT TACAACAGCG AGAATTACCT CACTCTCCTG 
GACGATGAAG ATCACACTTT AGAAAGCTTG AAAATTCCAG ATGAACAGCA CTTAGTCATT 
GAGGTTCGAA ACAAAGATAT GAGCTGGCCA GAGGAAATGT CTTTCATTGC AAACAGCAGC 
AAAATCGACA GACACAAAGT TCCTACTGAG AAAGGTGCCA CAGGTTTGAG TAATCTGGGC 
AACACGTGCT TCATGAACTC CAGTATTCAG TGTGTAAGCA ATACCAAGCC CCTCACTGAG 
TACTTCATCT CAGGGCGACA CCTTTATGAG CTTAATAGAA CTAATCCAAT AGGAATGCGT 
GGTCACATGG CCAAATGCTA TGGTGATTTG GTACAGGAAC TATGGAGCGG CACTCAAAAG 
AATGTTGCGC CTTTGAAACT TAGGTGGACG ATAGCAAAGT ACGCGCCAAG ATTCAATGGC 
TTCCAGCAGC AAGACTCCCA GGAGCTTTTG GCCTTCCTGT TGGATGGGCT TCATGAAGAC 
TTGAATCGTG TGCATGAGAA GCCGTATGTG GAGCTCAAGG ACAGTGATGG ACGGCCGGAC 
CATGAGGTTG CTACAGAGGC CTGGGAAAAC CATTTGCGGA GAAATCGCTC TATCGTGGTG 
GACCTCTTCC ATGGGCAGCT CAGATCCCAG GTGAAGTGCA AGACCTGTGG CCACATAAGC 
GCACGCTTCG ACCCCTTTAA CTTCCTATCG CTGCCTCTGC CAATGGACAG CTCCATGCAC 
TTAGAAATCA CAGTCATCAA ACTTGACGGC TCCACTCCAG TGCGATACGG GCTCCGTCTC 
AACATGGATG AGAAGTACAT GGGGCTAAAA AAACAGCTGA GCGAGCTCTG TAGCCTGAAA 
CCTGAGCAGA TCCTACTGGC CGAAGTTCAC ACGTCCAACA TCAAGAACTT TCCTCTGGAT 
AACCAGAAGG TGCGACACTC TGTCAACGGC TTCCTCTGTG CGTTTGAGAT CCCTGTGCCT 
GGTTCACCCA CTTCAGTCAC TTCTCCTGTT CAAACAGAAA AACCACCTAT TGCCAATGGG 
ACAGCAGGAA ACAATCATAT TGGAAAGTCT GGCCTGATTC CTAATGGTAT GCCCAGTACA 
GTGGTCCCCT GCACCCTTGA GAAACCCCTC GCCAATGGTA TCCCCAATGG CCATGCTGTG 
CCAGTGCAGG ACAGCCCATT CATTGGCTAC ATTATTGCCA TGCACAGAAA GATGATGCGG 
ACGGAACTGT ACTTCCTGTC CTCTCAGAAG AACAGGCCCA GTCTGTTTGG TATGCCTCTG 
ATTGTGCCAT GCACAGTGCA CACGAGTAAG AAGGACCTGT ATGATGCTGT GTGGATTCAA 
GTGTCCCGAC TTGCAAGTCC ACTGCCGCCG CAGGAAGCCA GCAACCATGC TCAAGACTGT 
GATGACAGTT TGGGCTATCA GTACCCCTTT ACGCTGCGGG TGGTGCAGAA AGATGGTAAC 
TCCTGTGCCT GGTGTCCCTG GTACAGGTTC TGCAGAGGCT GCACAGTGGA CTGTAATGAA 
GACAGAGCAT CTTTGGGGAA TGCCTACATT GCTGTAGACT GGGATCCCAC CGCCTTGCAC 
CTTCGTTACC AGACGTCTCA GGAAAGGATA GTTGAAGAGC ATCCCAGTGT TGAGCAGAGT 
CGACGCGCTC AAGCAGAGCC CATCAGTCTG GACAGCTGTC TGAAAGCCTT CACCAGTGAA 
GAAGAGCTGG GAGAGGACGA GCTCTATTAC TGCTCCAAGT GTAAAACACA CCGCCTGGCC 
ACTAAGAAGC TGGACCTCTG GAGGCTGCCT CCTGTACTGA TTGTTCATTT GAAACGCTTC 
CAATTTGTAA ATGGTCGATG GATCAAGTCT CAGAAGATTG TGAAGTTTCC ACGCGAGAGC 
TTCGACCCCA GTGCGTTCCT TGCCCCACGG GAGGTGTCTC AGAGCCTTGG CAGCCTGCAG 
AGCCTGCAGA GCGAGTGCCT GGTGGAGGAG CTCCAGAGGC TGGAGAGAGG AGACTCCTCT 
GTGTCCTCCA TCTCCACCAC CACCTTGGCC AGTCCTCCCA TATCTGGCAG AGCCACCCCG 
TGCTCCATCA GGAGGGTCGG CAGTCCCCTG AGCAGAAACA GCAGTCCCGG CAGCAGCCCC 
AGAAACACAG GCCGAAAACA GGGCCGCTTG CGTCTTCCCC AGCTGGGCAG CAGACATCGA 
CTCTGCAACA GCAAAGAAAA CCTGGAAGCC TGTAGCACCA ACAAGGAGAG CGAGAGCGAG 
GCCGAGCAAA GCGCAGTGAC GGACGGGGAG AATCTGACCC CTGATGCCAA CACACAAGAC 
ACCTGGTCAC TAGACGCCTC CAGCAACGAC TGCGATGTCA TTATGGTGAA TGGACTAGGA 
CAGGACAGCA GTCAGAGCAA TGGACATTCA GAGGTCAGCG CCGATCTGGA CACCCCTCAC 
CAAAGAAATG ACATCTGCCT GGAGTCCCTG TATAATTTAT ATGCAATCTC ATGCCATTCG 
GGAATAATGG GAGGGGGGCA CTATGTGACT TATGCCAAAA ACCCCAACGA AAAATGGTAC 
TGTTACAATG ACAGCAGCTG CAAGGAAGTG CACTCGGACG AGATTGACAC AGATTCAGCC 
TACATCCTGT TTTATGAGCA ACAGGGAGTG GACTACTCGC AGTTTCTGCC AAAAACGGAG 
GGCAAGAAGA TGGCAGACAC CACTAGCATG GATGAAGACT TTGAATCTGA CTACAAGAAG 
TACTGTGTGC TGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp32 ( XM_681144.9 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_686236.6 Danio rerio ubiquitin specific peptidase 32 (usp32), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa20507
Clone ID Related Accession (Same CDS sequence)	XM_681144.8 , XM_681144.9
Accession Version	XM_681144.9 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4758bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ubiquitin carboxyl-terminal hydrolase 32 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_681144.8. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGCCA AAGAGTCACG GATAGGCTTC CTTTCGTATG ACGAGGCCGT TAAAAGAGTT 
ACCGATGTGG AGCTCAAACG TCTGCGAGAT GCCTTTAAGA GGACCAGTGG CCTGTCAGTG 
TATATGACCC AGCAATGCTT CTTCAGAGAA GTCCTGGGCG ACGGAGTGCC ACCAAAGGTT 
GCAGAAGTGA TCTACAACTC ATTTGGTGGC ACATCCAAAG GGCTACACTT CAACTATCTG 
ATCGTCGGCC TGGTTTTGTT AACAAGAGGG CGTGATGAAG AGAAAGCCAA ATACATCTTC 
AGCTTGTTCG CGAGTGATTC TGGCACCTTT GTGGTCCGGG AGGATGTGGA GAACATGCTG 
CGCTCAGTGG ATGGGGAGGT CCCGCCTTCT CTCAGGAAGT GCTTTACTGA GGGGGAGAAG 
GTGAATTATG AGAAGTTTAA GAGCTGGCTG CTTCAGAATA AAGATGCTTT CACGTTCTCC 
CGCTGGCTGC TGTCAGGTGG TGTGTGCGTA ACGTTGACTG ACGACAGCGA CACACCCACC 
TTCTATCAAA CCCTGGCAGG TGTCACTCAC TTAGAAGAAT CAGACATTAT CGACTTGGAA 
AAACGCTACT GGCTGCTGAA AGCTCAATCA AGGACAGGCC GCTTTGATTT GGAAACCTTT 
GTCCCGCTGG TCTCGCCCCC TATTCATGCA TCGCTGAGTG AAGGCTTGTT TCACGCCTTT 
GATGAGAATC GGGACAATCA TATTGACTTT AAGGAGATAT CCTGTGGGCT CTCAGCTTGC 
TGTCGCGGCC CAGTAGCGGA GAGGCAGAAG TTTTGTTTTA AAGTGTTCGA CGTAGATCGT 
GATGGAGTTT TGTCCAGGGA CGAGATCCAC GAGATGGTGG TGGCGCTGCT GGAGGTGTGG 
AAGGATAACC GAACAGACAC TCTTCCTGAG TTTGATTCCA GTGTGTCAGA GATTGTGGAG 
GACATATTGA AAATGCACGA CACAACAAAA CAAGGACATT TAACTCTAGA GGACTATCAG 
ATATGGAGTG TGAAAAGTGC CCTCGCCAAT GAGTTCCTCA ACTTGCTGTT TCAGGTGTGT 
CATATTGTCC TGGGCCTGCG TCCTGCCACC CCTGAAGAGG AAGGCCAGAT CATTAGGGGT 
TGGTTGGAGA GGGAAAGCAG GCACGGTTTA CAGCCCAGTC AAAACTGGTT CCTGATCTCC 
ATGCAGTGGT GGCAGCAATG GAAAGACTAT GTCAGATATG ATAATAAGTC GATTGTCATG 
GAGCAGCCAT CAGTGCAGGG CAGCGGGATG AGAAGTTCTC AGTCTGCTGC ATCTGTAGAG 
CCTATCCTCA ACAGAATTGG ATTAGTGGTT CGCTCCCCCA GTTCCCCAGA AGAAAAATTC 
CCTGACAACA TCTCCAGCTC ATCAGAGGCT TCAGAGAGCA CTGGTAATAG CCTAACCCCA 
CACATCAGCT CCTCTGCCAC AGACGTGTGC TTTGCTCGGC AGCACAACAT CTCTGACAAC 
AACAACCAGT GTTTCTCAAG CGCCAATGGC CATTTGCCTA ACAGCTTGGC CGTGCAGAGA 
CCCGGTGCGA TAGACAACCA GCCTCTGGTG ACCACCGAGC CCATGAAGGC ACCCACGCTG 
ACTATGGAAG GAGGTCGACT GAAGAAGTCC CATCAACTGT TGTCTGGCCG GGAGTTTGAG 
ACGGTTCCAG AGCCGGTGTG GAGAGCACTT TACCACTGGT ATGGGGCCAA CCTTAGTCTG 
CCCCGACCGG TTATCAAGTC TACAGTCAAG GCACAGCCTG AGCTAGAGCT CTTCCCCCTT 
TACCTTCTGT TCCTGCGACA GCAACCGGCC ACACGGAGCC CTCAGTCCAA CATCTGGGTC 
AATATGGGTA ATGTTCCTTC TCCCAACGCC CCGCTCAAGC GAGTGCTGGC ATACACAGGC 
TGTTTCAGCA GAATGTGCAC CATTAAAGAC ATTCACGAGT ACCTGTCCCA GAGGTTACGC 
ATCAAAGAAG AGGACATGCG CCTCTGGCTT TACAACAGCG AGAATTACCT CACTCTCCTG 
GACGATGAAG ATCACACTTT AGAAAGCTTG AAAATTCCAG ATGAACAGCA CTTAGTCATT 
GAGGTTCGAA ACAAAGATAT GAGCTGGCCA GAGGAAATGT CTTTCATTGC AAACAGCAGC 
AAAATCGACA GACACAAAGT TCCTACTGAG AAAGGTGCCA CAGGTTTGAG TAATCTGGGC 
AACACGTGCT TCATGAACTC CAGTATTCAG TGTGTAAGCA ATACCAAGCC CCTCACTGAG 
TACTTCATCT CAGGGCGACA CCTTTATGAG CTTAATAGAA CTAATCCAAT AGGAATGCGT 
GGTCACATGG CCAAATGCTA TGGTGATTTG GTACAGGAAC TATGGAGCGG CACTCAAAAG 
AATGTTGCGC CTTTGAAACT TAGGTGGACG ATAGCAAAGT ACGCGCCAAG ATTCAATGGC 
TTCCAGCAGC AAGACTCCCA GGAGCTTTTG GCCTTCCTGT TGGATGGGCT TCATGAAGAC 
TTGAATCGTG TGCATGAGAA GCCGTATGTG GAGCTCAAGG ACAGTGATGG ACGGCCGGAC 
CATGAGGTTG CTACAGAGGC CTGGGAAAAC CATTTGCGGA GAAATCGCTC TATCGTGGTG 
GACCTCTTCC ATGGGCAGCT CAGATCCCAG GTGAAGTGCA AGACCTGTGG CCACATAAGC 
GCACGCTTCG ACCCCTTTAA CTTCCTATCG CTGCCTCTGC CAATGGACAG CTCCATGCAC 
TTAGAAATCA CAGTCATCAA ACTTGACGGC TCCACTCCAG TGCGATACGG GCTCCGTCTC 
AACATGGATG AGAAGTACAT GGGGCTAAAA AAACAGCTGA GCGAGCTCTG TAGCCTGAAA 
CCTGAGCAGA TCCTACTGGC CGAAGTTCAC ACGTCCAACA TCAAGAACTT TCCTCTGGAT 
AACCAGAAGG TGCGACACTC TGTCAACGGC TTCCTCTGTG CGTTTGAGAT CCCTGTGCCT 
GGTTCACCCA CTTCAGTCAC TTCTCCTGTT CAAACAGAAA AACCACCTAT TGCCAATGGG 
ACAGCAGGAA ACAATCATAT TGGAAAGTCT GGCCTGATTC CTAATGGTAT GCCCAGTACA 
GTGGTCCCCT GCACCCTTGA GAAACCCCTC GCCAATGGTA TCCCCAATGG CCATGCTGTG 
CCAGTGCAGG ACAGCCCATT CATTGGCTAC ATTATTGCCA TGCACAGAAA GATGATGCGG 
ACGGAACTGT ACTTCCTGTC CTCTCAGAAG AACAGGCCCA GTCTGTTTGG TATGCCTCTG 
ATTGTGCCAT GCACAGTGCA CACGAGTAAG AAGGACCTGT ATGATGCTGT GTGGATTCAA 
GTGTCCCGAC TTGCAAGTCC ACTGCCGCCG CAGGAAGCCA GCAACCATGC TCAAGACTGT 
GATGACAGTT TGGGCTATCA GTACCCCTTT ACGCTGCGGG TGGTGCAGAA AGATGGTAAC 
TCCTGTGCCT GGTGTCCCTG GTACAGGTTC TGCAGAGGCT GCACAGTGGA CTGTAATGAA 
GACAGAGCAT CTTTGGGGAA TGCCTACATT GCTGTAGACT GGGATCCCAC CGCCTTGCAC 
CTTCGTTACC AGACGTCTCA GGAAAGGATA GTTGAAGAGC ATCCCAGTGT TGAGCAGAGT 
CGACGCGCTC AAGCAGAGCC CATCAGTCTG GACAGCTGTC TGAAAGCCTT CACCAGTGAA 
GAAGAGCTGG GAGAGGACGA GCTCTATTAC TGCTCCAAGT GTAAAACACA CCGCCTGGCC 
ACTAAGAAGC TGGACCTCTG GAGGCTGCCT CCTGTACTGA TTGTTCATTT GAAACGCTTC 
CAATTTGTAA ATGGTCGATG GATCAAGTCT CAGAAGATTG TGAAGTTTCC ACGCGAGAGC 
TTCGACCCCA GTGCGTTCCT TGCCCCACGG GAGGTGTCTC AGAGCCTTGG CAGCCTGCAG 
AGCCTGCAGA GCGAGTGCCT GGTGGAGGAG CTCCAGAGGC TGGAGAGAGG AGACTCCTCT 
GTGTCCTCCA TCTCCACCAC CACCTTGGCC AGTCCTCCCA TATCTGGCAG AGCCACCCCG 
TGCTCCATCA GGAGGGTCGG CAGTCCCCTG AGCAGAAACA GCAGTCCCGG CAGCAGCCCC 
AGAAACACAG GCCGAAAACA GGGCCGCTTG CGTCTTCCCC AGCTGGGCAG CAGACATCGA 
CTCTGCAACA GCAAAGAAAA CCTGGAAGCC TGTAGCACCA ACAAGGAGAG CGAGAGCGAG 
GCCGAGCAAA GCGCAGTGAC GGACGGGGAG AATCTGACCC CTGATGCCAA CACACAAGAC 
ACCTGGTCAC TAGACGCCTC CAGCAACGAC TGCGATGTCA TTATGGTGAA TGGACTAGGA 
CAGGACAGCA GTCAGAGCAA TGGACATTCA GAGGTCAGCG CCGATCTGGA CACCCCTCAC 
CAAAGAAATG ACATCTGCCT GGAGTCCCTG TATAATTTAT ATGCAATCTC ATGCCATTCG 
GGAATAATGG GAGGGGGGCA CTATGTGACT TATGCCAAAA ACCCCAACGA AAAATGGTAC 
TGTTACAATG ACAGCAGCTG CAAGGAAGTG CACTCGGACG AGATTGACAC AGATTCAGCC 
TACATCCTGT TTTATGAGCA ACAGGGAGTG GACTACTCGC AGTTTCTGCC AAAAACGGAG 
GGCAAGAAGA TGGCAGACAC CACTAGCATG GATGAAGACT TTGAATCTGA CTACAAGAAG 
TACTGTGTGC TGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_686236.6
CDS	148..4905
Translation	MGAKESRIGFLSYDEAVKRVTDVELKRLRDAFKRTSGLSVYMTQQCFFREVLGDGVPPKVAEVIYNSFGGTSKGLHFNYLIVGLVLLTRGRDEEKAKYIFSLFASDSGTFVVREDVENMLRSVDGEVPPSLRKCFTEGEKVNYEKFKSWLLQNKDAFTFSRWLLSGGVCVTLTDDSDTPTFYQTLAGVTHLEESDIIDLEKRYWLLKAQSRTGRFDLETFVPLVSPPIHASLSEGLFHAFDENRDNHIDFKEISCGLSACCRGPVAERQKFCFKVFDVDRDGVLSRDEIHEMVVALLEVWKDNRTDTLPEFDSSVSEIVEDILKMHDTTKQGHLTLEDYQIWSVKSALANEFLNLLFQVCHIVLGLRPATPEEEGQIIRGWLERESRHGLQPSQNWFLISMQWWQQWKDYVRYDNKSIVMEQPSVQGSGMRSSQSAASVEPILNRIGLVVRSPSSPEEKFPDNISSSSEASESTGNSLTPHISSSATDVCFARQHNISDNNNQCFSSANGHLPNSLAVQRPGAIDNQPLVTTEPMKAPTLTMEGGRLKKSHQLLSGREFETVPEPVWRALYHWYGANLSLPRPVIKSTVKAQPELELFPLYLLFLRQQPATRSPQSNIWVNMGNVPSPNAPLKRVLAYTGCFSRMCTIKDIHEYLSQRLRIKEEDMRLWLYNSENYLTLLDDEDHTLESLKIPDEQHLVIEVRNKDMSWPEEMSFIANSSKIDRHKVPTEKGATGLSNLGNTCFMNSSIQCVSNTKPLTEYFISGRHLYELNRTNPIGMRGHMAKCYGDLVQELWSGTQKNVAPLKLRWTIAKYAPRFNGFQQQDSQELLAFLLDGLHEDLNRVHEKPYVELKDSDGRPDHEVATEAWENHLRRNRSIVVDLFHGQLRSQVKCKTCGHISARFDPFNFLSLPLPMDSSMHLEITVIKLDGSTPVRYGLRLNMDEKYMGLKKQLSELCSLKPEQILLAEVHTSNIKNFPLDNQKVRHSVNGFLCAFEIPVPGSPTSVTSPVQTEKPPIANGTAGNNHIGKSGLIPNGMPSTVVPCTLEKPLANGIPNGHAVPVQDSPFIGYIIAMHRKMMRTELYFLSSQKNRPSLFGMPLIVPCTVHTSKKDLYDAVWIQVSRLASPLPPQEASNHAQDCDDSLGYQYPFTLRVVQKDGNSCAWCPWYRFCRGCTVDCNEDRASLGNAYIAVDWDPTALHLRYQTSQERIVEEHPSVEQSRRAQAEPISLDSCLKAFTSEEELGEDELYYCSKCKTHRLATKKLDLWRLPPVLIVHLKRFQFVNGRWIKSQKIVKFPRESFDPSAFLAPREVSQSLGSLQSLQSECLVEELQRLERGDSSVSSISTTTLASPPISGRATPCSIRRVGSPLSRNSSPGSSPRNTGRKQGRLRLPQLGSRHRLCNSKENLEACSTNKESESEAEQSAVTDGENLTPDANTQDTWSLDASSNDCDVIMVNGLGQDSSQSNGHSEVSADLDTPHQRNDICLESLYNLYAISCHSGIMGGGHYVTYAKNPNEKWYCYNDSSCKEVHSDEIDTDSAYILFYEQQGVDYSQFLPKTEGKKMADTTSMDEDFESDYKKYCVLQ

Target ORF information:

RefSeq Version	XM_681144.9
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ubiquitin specific peptidase 32 (usp32), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_681144.9

ORF Insert Sequence:

ATGGGCGCCA AAGAGTCACG GATAGGCTTC CTTTCGTATG ACGAGGCCGT TAAAAGAGTT 
ACCGATGTGG AGCTCAAACG TCTGCGAGAT GCCTTTAAGA GGACCAGTGG CCTGTCAGTG 
TATATGACCC AGCAATGCTT CTTCAGAGAA GTCCTGGGCG ACGGAGTGCC ACCAAAGGTT 
GCAGAAGTGA TCTACAACTC ATTTGGTGGC ACATCCAAAG GGCTACACTT CAACTATCTG 
ATCGTCGGCC TGGTTTTGTT AACAAGAGGG CGTGATGAAG AGAAAGCCAA ATACATCTTC 
AGCTTGTTCG CGAGTGATTC TGGCACCTTT GTGGTCCGGG AGGATGTGGA GAACATGCTG 
CGCTCAGTGG ATGGGGAGGT CCCGCCTTCT CTCAGGAAGT GCTTTACTGA GGGGGAGAAG 
GTGAATTATG AGAAGTTTAA GAGCTGGCTG CTTCAGAATA AAGATGCTTT CACGTTCTCC 
CGCTGGCTGC TGTCAGGTGG TGTGTGCGTA ACGTTGACTG ACGACAGCGA CACACCCACC 
TTCTATCAAA CCCTGGCAGG TGTCACTCAC TTAGAAGAAT CAGACATTAT CGACTTGGAA 
AAACGCTACT GGCTGCTGAA AGCTCAATCA AGGACAGGCC GCTTTGATTT GGAAACCTTT 
GTCCCGCTGG TCTCGCCCCC TATTCATGCA TCGCTGAGTG AAGGCTTGTT TCACGCCTTT 
GATGAGAATC GGGACAATCA TATTGACTTT AAGGAGATAT CCTGTGGGCT CTCAGCTTGC 
TGTCGCGGCC CAGTAGCGGA GAGGCAGAAG TTTTGTTTTA AAGTGTTCGA CGTAGATCGT 
GATGGAGTTT TGTCCAGGGA CGAGATCCAC GAGATGGTGG TGGCGCTGCT GGAGGTGTGG 
AAGGATAACC GAACAGACAC TCTTCCTGAG TTTGATTCCA GTGTGTCAGA GATTGTGGAG 
GACATATTGA AAATGCACGA CACAACAAAA CAAGGACATT TAACTCTAGA GGACTATCAG 
ATATGGAGTG TGAAAAGTGC CCTCGCCAAT GAGTTCCTCA ACTTGCTGTT TCAGGTGTGT 
CATATTGTCC TGGGCCTGCG TCCTGCCACC CCTGAAGAGG AAGGCCAGAT CATTAGGGGT 
TGGTTGGAGA GGGAAAGCAG GCACGGTTTA CAGCCCAGTC AAAACTGGTT CCTGATCTCC 
ATGCAGTGGT GGCAGCAATG GAAAGACTAT GTCAGATATG ATAATAAGTC GATTGTCATG 
GAGCAGCCAT CAGTGCAGGG CAGCGGGATG AGAAGTTCTC AGTCTGCTGC ATCTGTAGAG 
CCTATCCTCA ACAGAATTGG ATTAGTGGTT CGCTCCCCCA GTTCCCCAGA AGAAAAATTC 
CCTGACAACA TCTCCAGCTC ATCAGAGGCT TCAGAGAGCA CTGGTAATAG CCTAACCCCA 
CACATCAGCT CCTCTGCCAC AGACGTGTGC TTTGCTCGGC AGCACAACAT CTCTGACAAC 
AACAACCAGT GTTTCTCAAG CGCCAATGGC CATTTGCCTA ACAGCTTGGC CGTGCAGAGA 
CCCGGTGCGA TAGACAACCA GCCTCTGGTG ACCACCGAGC CCATGAAGGC ACCCACGCTG 
ACTATGGAAG GAGGTCGACT GAAGAAGTCC CATCAACTGT TGTCTGGCCG GGAGTTTGAG 
ACGGTTCCAG AGCCGGTGTG GAGAGCACTT TACCACTGGT ATGGGGCCAA CCTTAGTCTG 
CCCCGACCGG TTATCAAGTC TACAGTCAAG GCACAGCCTG AGCTAGAGCT CTTCCCCCTT 
TACCTTCTGT TCCTGCGACA GCAACCGGCC ACACGGAGCC CTCAGTCCAA CATCTGGGTC 
AATATGGGTA ATGTTCCTTC TCCCAACGCC CCGCTCAAGC GAGTGCTGGC ATACACAGGC 
TGTTTCAGCA GAATGTGCAC CATTAAAGAC ATTCACGAGT ACCTGTCCCA GAGGTTACGC 
ATCAAAGAAG AGGACATGCG CCTCTGGCTT TACAACAGCG AGAATTACCT CACTCTCCTG 
GACGATGAAG ATCACACTTT AGAAAGCTTG AAAATTCCAG ATGAACAGCA CTTAGTCATT 
GAGGTTCGAA ACAAAGATAT GAGCTGGCCA GAGGAAATGT CTTTCATTGC AAACAGCAGC 
AAAATCGACA GACACAAAGT TCCTACTGAG AAAGGTGCCA CAGGTTTGAG TAATCTGGGC 
AACACGTGCT TCATGAACTC CAGTATTCAG TGTGTAAGCA ATACCAAGCC CCTCACTGAG 
TACTTCATCT CAGGGCGACA CCTTTATGAG CTTAATAGAA CTAATCCAAT AGGAATGCGT 
GGTCACATGG CCAAATGCTA TGGTGATTTG GTACAGGAAC TATGGAGCGG CACTCAAAAG 
AATGTTGCGC CTTTGAAACT TAGGTGGACG ATAGCAAAGT ACGCGCCAAG ATTCAATGGC 
TTCCAGCAGC AAGACTCCCA GGAGCTTTTG GCCTTCCTGT TGGATGGGCT TCATGAAGAC 
TTGAATCGTG TGCATGAGAA GCCGTATGTG GAGCTCAAGG ACAGTGATGG ACGGCCGGAC 
CATGAGGTTG CTACAGAGGC CTGGGAAAAC CATTTGCGGA GAAATCGCTC TATCGTGGTG 
GACCTCTTCC ATGGGCAGCT CAGATCCCAG GTGAAGTGCA AGACCTGTGG CCACATAAGC 
GCACGCTTCG ACCCCTTTAA CTTCCTATCG CTGCCTCTGC CAATGGACAG CTCCATGCAC 
TTAGAAATCA CAGTCATCAA ACTTGACGGC TCCACTCCAG TGCGATACGG GCTCCGTCTC 
AACATGGATG AGAAGTACAT GGGGCTAAAA AAACAGCTGA GCGAGCTCTG TAGCCTGAAA 
CCTGAGCAGA TCCTACTGGC CGAAGTTCAC ACGTCCAACA TCAAGAACTT TCCTCTGGAT 
AACCAGAAGG TGCGACACTC TGTCAACGGC TTCCTCTGTG CGTTTGAGAT CCCTGTGCCT 
GGTTCACCCA CTTCAGTCAC TTCTCCTGTT CAAACAGAAA AACCACCTAT TGCCAATGGG 
ACAGCAGGAA ACAATCATAT TGGAAAGTCT GGCCTGATTC CTAATGGTAT GCCCAGTACA 
GTGGTCCCCT GCACCCTTGA GAAACCCCTC GCCAATGGTA TCCCCAATGG CCATGCTGTG 
CCAGTGCAGG ACAGCCCATT CATTGGCTAC ATTATTGCCA TGCACAGAAA GATGATGCGG 
ACGGAACTGT ACTTCCTGTC CTCTCAGAAG AACAGGCCCA GTCTGTTTGG TATGCCTCTG 
ATTGTGCCAT GCACAGTGCA CACGAGTAAG AAGGACCTGT ATGATGCTGT GTGGATTCAA 
GTGTCCCGAC TTGCAAGTCC ACTGCCGCCG CAGGAAGCCA GCAACCATGC TCAAGACTGT 
GATGACAGTT TGGGCTATCA GTACCCCTTT ACGCTGCGGG TGGTGCAGAA AGATGGTAAC 
TCCTGTGCCT GGTGTCCCTG GTACAGGTTC TGCAGAGGCT GCACAGTGGA CTGTAATGAA 
GACAGAGCAT CTTTGGGGAA TGCCTACATT GCTGTAGACT GGGATCCCAC CGCCTTGCAC 
CTTCGTTACC AGACGTCTCA GGAAAGGATA GTTGAAGAGC ATCCCAGTGT TGAGCAGAGT 
CGACGCGCTC AAGCAGAGCC CATCAGTCTG GACAGCTGTC TGAAAGCCTT CACCAGTGAA 
GAAGAGCTGG GAGAGGACGA GCTCTATTAC TGCTCCAAGT GTAAAACACA CCGCCTGGCC 
ACTAAGAAGC TGGACCTCTG GAGGCTGCCT CCTGTACTGA TTGTTCATTT GAAACGCTTC 
CAATTTGTAA ATGGTCGATG GATCAAGTCT CAGAAGATTG TGAAGTTTCC ACGCGAGAGC 
TTCGACCCCA GTGCGTTCCT TGCCCCACGG GAGGTGTCTC AGAGCCTTGG CAGCCTGCAG 
AGCCTGCAGA GCGAGTGCCT GGTGGAGGAG CTCCAGAGGC TGGAGAGAGG AGACTCCTCT 
GTGTCCTCCA TCTCCACCAC CACCTTGGCC AGTCCTCCCA TATCTGGCAG AGCCACCCCG 
TGCTCCATCA GGAGGGTCGG CAGTCCCCTG AGCAGAAACA GCAGTCCCGG CAGCAGCCCC 
AGAAACACAG GCCGAAAACA GGGCCGCTTG CGTCTTCCCC AGCTGGGCAG CAGACATCGA 
CTCTGCAACA GCAAAGAAAA CCTGGAAGCC TGTAGCACCA ACAAGGAGAG CGAGAGCGAG 
GCCGAGCAAA GCGCAGTGAC GGACGGGGAG AATCTGACCC CTGATGCCAA CACACAAGAC 
ACCTGGTCAC TAGACGCCTC CAGCAACGAC TGCGATGTCA TTATGGTGAA TGGACTAGGA 
CAGGACAGCA GTCAGAGCAA TGGACATTCA GAGGTCAGCG CCGATCTGGA CACCCCTCAC 
CAAAGAAATG ACATCTGCCT GGAGTCCCTG TATAATTTAT ATGCAATCTC ATGCCATTCG 
GGAATAATGG GAGGGGGGCA CTATGTGACT TATGCCAAAA ACCCCAACGA AAAATGGTAC 
TGTTACAATG ACAGCAGCTG CAAGGAAGTG CACTCGGACG AGATTGACAC AGATTCAGCC 
TACATCCTGT TTTATGAGCA ACAGGGAGTG GACTACTCGC AGTTTCTGCC AAAAACGGAG 
GGCAAGAAGA TGGCAGACAC CACTAGCATG GATGAAGACT TTGAATCTGA CTACAAGAAG 
TACTGTGTGC TGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp32 ( XM_005157606.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005157663.1 Danio rerio ubiquitin specific peptidase 32 (usp32), transcript variant X2, mRNA.

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CloneID	ODa20508
Clone ID Related Accession (Same CDS sequence)	XM_005157606.3 , XM_005157606.4
Accession Version	XM_005157606.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4752bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ubiquitin carboxyl-terminal hydrolase 32 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005157606.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGCCA AAGAGTCACG GATAGGCTTC CTTTCGTATG ACGAGGCCGT TAAAAGAGTT 
ACCGATGTGG AGCTCAAACG TCTGCGAGAT GCCTTTAAGA GGACCAGTGG CCTGTCAGTG 
TATATGACCC AGCAATGCTT CTTCAGAGAA GTCCTGGGCG ACGGAGTGCC ACCAAAGGTT 
GCAGAAGTGA TCTACAACTC ATTTGGTGGC ACATCCAAAG GGCTACACTT CAACTATCTG 
ATCGTCGGCC TGGTTTTGTT AACAAGAGGG CGTGATGAAG AGAAAGCCAA ATACATCTTC 
AGCTTGTTCG CGAGTGATTC TGGCACCTTT GTGGTCCGGG AGGATGTGGA GAACATGCTG 
CGCTCAGTGG ATGGGGAGGT CCCGCCTTCT CTCAGGAAGT GCTTTACTGA GGGGGAGAAG 
GTGAATTATG AGAAGTTTAA GAGCTGGCTG CTTCAGAATA AAGATGCTTT CACGTTCTCC 
CGCTGGCTGC TGTCAGGTGG TGTGTGCGTA ACGTTGACTG ACGACAGCGA CACACCCACC 
TTCTATCAAA CCCTGGCAGG TGTCACTCAC TTAGAAGAAT CAGACATTAT CGACTTGGAA 
AAACGCTACT GGCTGCTGAA AGCTCAATCA AGGACAGGCC GCTTTGATTT GGAAACCTTT 
GTCCCGCTGG TCTCGCCCCC TATTCATGCA TCGCTGAGTG AAGGCTTGTT TCACGCCTTT 
GATGAGAATC GGGACAATCA TATTGACTTT AAGGAGATAT CCTGTGGGCT CTCAGCTTGC 
TGTCGCGGCC CAGTAGCGGA GAGGCAGAAG TTTTGTTTTA AAGTGTTCGA CGTAGATCGT 
GATGGAGTTT TGTCCAGGGA CGAGATCCAC GAGATGGTGG TGGCGCTGCT GGAGGTGTGG 
AAGGATAACC GAACAGACAC TCTTCCTGAG TTTGATTCCA GTGTGTCAGA GATTGTGGAG 
GACATATTGA AAATGCACGA CACAACAAAA CAAGGACATT TAACTCTAGA GGACTATCAG 
ATATGGAGTG TGAAAAGTGC CCTCGCCAAT GAGTTCCTCA ACTTGCTGTT TCAGGTGTGT 
CATATTGTCC TGGGCCTGCG TCCTGCCACC CCTGAAGAGG AAGGCCAGAT CATTAGGGGT 
TGGTTGGAGA GGGAAAGCAG GCACGGTTTA CAGCCCAGTC AAAACTGGTT CCTGATCTCC 
ATGCAGTGGT GGCAGCAATG GAAAGACTAT GTCAGATATG ATAATAAGTC GATTGTCATG 
GAGCAGCCAT CAGTGCAGGG CAGCGGGATG AGAAGTTCTC AGTCTGCTGC ATCTGTAGAG 
CCTATCCTCA ACAGAATTGG ATTAGTGGTT CGCTCCCCCA GTTCCCCAGA AGAAAAATTC 
CCTGACAACA TCTCCAGCTC ATCAGAGGCT TCAGAGAGCA CTGGCCTAAC CCCACACATC 
AGCTCCTCTG CCACAGACGT GTGCTTTGCT CGGCAGCACA ACATCTCTGA CAACAACAAC 
CAGTGTTTCT CAAGCGCCAA TGGCCATTTG CCTAACAGCT TGGCCGTGCA GAGACCCGGT 
GCGATAGACA ACCAGCCTCT GGTGACCACC GAGCCCATGA AGGCACCCAC GCTGACTATG 
GAAGGAGGTC GACTGAAGAA GTCCCATCAA CTGTTGTCTG GCCGGGAGTT TGAGACGGTT 
CCAGAGCCGG TGTGGAGAGC ACTTTACCAC TGGTATGGGG CCAACCTTAG TCTGCCCCGA 
CCGGTTATCA AGTCTACAGT CAAGGCACAG CCTGAGCTAG AGCTCTTCCC CCTTTACCTT 
CTGTTCCTGC GACAGCAACC GGCCACACGG AGCCCTCAGT CCAACATCTG GGTCAATATG 
GGTAATGTTC CTTCTCCCAA CGCCCCGCTC AAGCGAGTGC TGGCATACAC AGGCTGTTTC 
AGCAGAATGT GCACCATTAA AGACATTCAC GAGTACCTGT CCCAGAGGTT ACGCATCAAA 
GAAGAGGACA TGCGCCTCTG GCTTTACAAC AGCGAGAATT ACCTCACTCT CCTGGACGAT 
GAAGATCACA CTTTAGAAAG CTTGAAAATT CCAGATGAAC AGCACTTAGT CATTGAGGTT 
CGAAACAAAG ATATGAGCTG GCCAGAGGAA ATGTCTTTCA TTGCAAACAG CAGCAAAATC 
GACAGACACA AAGTTCCTAC TGAGAAAGGT GCCACAGGTT TGAGTAATCT GGGCAACACG 
TGCTTCATGA ACTCCAGTAT TCAGTGTGTA AGCAATACCA AGCCCCTCAC TGAGTACTTC 
ATCTCAGGGC GACACCTTTA TGAGCTTAAT AGAACTAATC CAATAGGAAT GCGTGGTCAC 
ATGGCCAAAT GCTATGGTGA TTTGGTACAG GAACTATGGA GCGGCACTCA AAAGAATGTT 
GCGCCTTTGA AACTTAGGTG GACGATAGCA AAGTACGCGC CAAGATTCAA TGGCTTCCAG 
CAGCAAGACT CCCAGGAGCT TTTGGCCTTC CTGTTGGATG GGCTTCATGA AGACTTGAAT 
CGTGTGCATG AGAAGCCGTA TGTGGAGCTC AAGGACAGTG ATGGACGGCC GGACCATGAG 
GTTGCTACAG AGGCCTGGGA AAACCATTTG CGGAGAAATC GCTCTATCGT GGTGGACCTC 
TTCCATGGGC AGCTCAGATC CCAGGTGAAG TGCAAGACCT GTGGCCACAT AAGCGCACGC 
TTCGACCCCT TTAACTTCCT ATCGCTGCCT CTGCCAATGG ACAGCTCCAT GCACTTAGAA 
ATCACAGTCA TCAAACTTGA CGGCTCCACT CCAGTGCGAT ACGGGCTCCG TCTCAACATG 
GATGAGAAGT ACATGGGGCT AAAAAAACAG CTGAGCGAGC TCTGTAGCCT GAAACCTGAG 
CAGATCCTAC TGGCCGAAGT TCACACGTCC AACATCAAGA ACTTTCCTCT GGATAACCAG 
AAGGTGCGAC ACTCTGTCAA CGGCTTCCTC TGTGCGTTTG AGATCCCTGT GCCTGGTTCA 
CCCACTTCAG TCACTTCTCC TGTTCAAACA GAAAAACCAC CTATTGCCAA TGGGACAGCA 
GGAAACAATC ATATTGGAAA GTCTGGCCTG ATTCCTAATG GTATGCCCAG TACAGTGGTC 
CCCTGCACCC TTGAGAAACC CCTCGCCAAT GGTATCCCCA ATGGCCATGC TGTGCCAGTG 
CAGGACAGCC CATTCATTGG CTACATTATT GCCATGCACA GAAAGATGAT GCGGACGGAA 
CTGTACTTCC TGTCCTCTCA GAAGAACAGG CCCAGTCTGT TTGGTATGCC TCTGATTGTG 
CCATGCACAG TGCACACGAG TAAGAAGGAC CTGTATGATG CTGTGTGGAT TCAAGTGTCC 
CGACTTGCAA GTCCACTGCC GCCGCAGGAA GCCAGCAACC ATGCTCAAGA CTGTGATGAC 
AGTTTGGGCT ATCAGTACCC CTTTACGCTG CGGGTGGTGC AGAAAGATGG TAACTCCTGT 
GCCTGGTGTC CCTGGTACAG GTTCTGCAGA GGCTGCACAG TGGACTGTAA TGAAGACAGA 
GCATCTTTGG GGAATGCCTA CATTGCTGTA GACTGGGATC CCACCGCCTT GCACCTTCGT 
TACCAGACGT CTCAGGAAAG GATAGTTGAA GAGCATCCCA GTGTTGAGCA GAGTCGACGC 
GCTCAAGCAG AGCCCATCAG TCTGGACAGC TGTCTGAAAG CCTTCACCAG TGAAGAAGAG 
CTGGGAGAGG ACGAGCTCTA TTACTGCTCC AAGTGTAAAA CACACCGCCT GGCCACTAAG 
AAGCTGGACC TCTGGAGGCT GCCTCCTGTA CTGATTGTTC ATTTGAAACG CTTCCAATTT 
GTAAATGGTC GATGGATCAA GTCTCAGAAG ATTGTGAAGT TTCCACGCGA GAGCTTCGAC 
CCCAGTGCGT TCCTTGCCCC ACGGGAGGTG TCTCAGAGCC TTGGCAGCCT GCAGAGCCTG 
CAGAGCGAGT GCCTGGTGGA GGAGCTCCAG AGGCTGGAGA GAGGAGACTC CTCTGTGTCC 
TCCATCTCCA CCACCACCTT GGCCAGTCCT CCCATATCTG GCAGAGCCAC CCCGTGCTCC 
ATCAGGAGGG TCGGCAGTCC CCTGAGCAGA AACAGCAGTC CCGGCAGCAG CCCCAGAAAC 
ACAGGCCGAA AACAGGGCCG CTTGCGTCTT CCCCAGCTGG GCAGCAGACA TCGACTCTGC 
AACAGCAAAG AAAACCTGGA AGCCTGTAGC ACCAACAAGG AGAGCGAGAG CGAGGCCGAG 
CAAAGCGCAG TGACGGACGG GGAGAATCTG ACCCCTGATG CCAACACACA AGACACCTGG 
TCACTAGACG CCTCCAGCAA CGACTGCGAT GTCATTATGG TGAATGGACT AGGACAGGAC 
AGCAGTCAGA GCAATGGACA TTCAGAGGTC AGCGCCGATC TGGACACCCC TCACCAAAGA 
AATGACATCT GCCTGGAGTC CCTGTATAAT TTATATGCAA TCTCATGCCA TTCGGGAATA 
ATGGGAGGGG GGCACTATGT GACTTATGCC AAAAACCCCA ACGAAAAATG GTACTGTTAC 
AATGACAGCA GCTGCAAGGA AGTGCACTCG GACGAGATTG ACACAGATTC AGCCTACATC 
CTGTTTTATG AGCAACAGGG AGTGGACTAC TCGCAGTTTC TGCCAAAAAC GGAGGGCAAG 
AAGATGGCAG ACACCACTAG CATGGATGAA GACTTTGAAT CTGACTACAA GAAGTACTGT 
GTGCTGCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005157663.1
CDS	160..4911
Translation	MGAKESRIGFLSYDEAVKRVTDVELKRLRDAFKRTSGLSVYMTQQCFFREVLGDGVPPKVAEVIYNSFGGTSKGLHFNYLIVGLVLLTRGRDEEKAKYIFSLFASDSGTFVVREDVENMLRSVDGEVPPSLRKCFTEGEKVNYEKFKSWLLQNKDAFTFSRWLLSGGVCVTLTDDSDTPTFYQTLAGVTHLEESDIIDLEKRYWLLKAQSRTGRFDLETFVPLVSPPIHASLSEGLFHAFDENRDNHIDFKEISCGLSACCRGPVAERQKFCFKVFDVDRDGVLSRDEIHEMVVALLEVWKDNRTDTLPEFDSSVSEIVEDILKMHDTTKQGHLTLEDYQIWSVKSALANEFLNLLFQVCHIVLGLRPATPEEEGQIIRGWLERESRHGLQPSQNWFLISMQWWQQWKDYVRYDNKSIVMEQPSVQGSGMRSSQSAASVEPILNRIGLVVRSPSSPEEKFPDNISSSSEASESTGLTPHISSSATDVCFARQHNISDNNNQCFSSANGHLPNSLAVQRPGAIDNQPLVTTEPMKAPTLTMEGGRLKKSHQLLSGREFETVPEPVWRALYHWYGANLSLPRPVIKSTVKAQPELELFPLYLLFLRQQPATRSPQSNIWVNMGNVPSPNAPLKRVLAYTGCFSRMCTIKDIHEYLSQRLRIKEEDMRLWLYNSENYLTLLDDEDHTLESLKIPDEQHLVIEVRNKDMSWPEEMSFIANSSKIDRHKVPTEKGATGLSNLGNTCFMNSSIQCVSNTKPLTEYFISGRHLYELNRTNPIGMRGHMAKCYGDLVQELWSGTQKNVAPLKLRWTIAKYAPRFNGFQQQDSQELLAFLLDGLHEDLNRVHEKPYVELKDSDGRPDHEVATEAWENHLRRNRSIVVDLFHGQLRSQVKCKTCGHISARFDPFNFLSLPLPMDSSMHLEITVIKLDGSTPVRYGLRLNMDEKYMGLKKQLSELCSLKPEQILLAEVHTSNIKNFPLDNQKVRHSVNGFLCAFEIPVPGSPTSVTSPVQTEKPPIANGTAGNNHIGKSGLIPNGMPSTVVPCTLEKPLANGIPNGHAVPVQDSPFIGYIIAMHRKMMRTELYFLSSQKNRPSLFGMPLIVPCTVHTSKKDLYDAVWIQVSRLASPLPPQEASNHAQDCDDSLGYQYPFTLRVVQKDGNSCAWCPWYRFCRGCTVDCNEDRASLGNAYIAVDWDPTALHLRYQTSQERIVEEHPSVEQSRRAQAEPISLDSCLKAFTSEEELGEDELYYCSKCKTHRLATKKLDLWRLPPVLIVHLKRFQFVNGRWIKSQKIVKFPRESFDPSAFLAPREVSQSLGSLQSLQSECLVEELQRLERGDSSVSSISTTTLASPPISGRATPCSIRRVGSPLSRNSSPGSSPRNTGRKQGRLRLPQLGSRHRLCNSKENLEACSTNKESESEAEQSAVTDGENLTPDANTQDTWSLDASSNDCDVIMVNGLGQDSSQSNGHSEVSADLDTPHQRNDICLESLYNLYAISCHSGIMGGGHYVTYAKNPNEKWYCYNDSSCKEVHSDEIDTDSAYILFYEQQGVDYSQFLPKTEGKKMADTTSMDEDFESDYKKYCVLQ

Target ORF information:

RefSeq Version	XM_005157606.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ubiquitin specific peptidase 32 (usp32), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005157606.3

ORF Insert Sequence:

ATGGGCGCCA AAGAGTCACG GATAGGCTTC CTTTCGTATG ACGAGGCCGT TAAAAGAGTT 
ACCGATGTGG AGCTCAAACG TCTGCGAGAT GCCTTTAAGA GGACCAGTGG CCTGTCAGTG 
TATATGACCC AGCAATGCTT CTTCAGAGAA GTCCTGGGCG ACGGAGTGCC ACCAAAGGTT 
GCAGAAGTGA TCTACAACTC ATTTGGTGGC ACATCCAAAG GGCTACACTT CAACTATCTG 
ATCGTCGGCC TGGTTTTGTT AACAAGAGGG CGTGATGAAG AGAAAGCCAA ATACATCTTC 
AGCTTGTTCG CGAGTGATTC TGGCACCTTT GTGGTCCGGG AGGATGTGGA GAACATGCTG 
CGCTCAGTGG ATGGGGAGGT CCCGCCTTCT CTCAGGAAGT GCTTTACTGA GGGGGAGAAG 
GTGAATTATG AGAAGTTTAA GAGCTGGCTG CTTCAGAATA AAGATGCTTT CACGTTCTCC 
CGCTGGCTGC TGTCAGGTGG TGTGTGCGTA ACGTTGACTG ACGACAGCGA CACACCCACC 
TTCTATCAAA CCCTGGCAGG TGTCACTCAC TTAGAAGAAT CAGACATTAT CGACTTGGAA 
AAACGCTACT GGCTGCTGAA AGCTCAATCA AGGACAGGCC GCTTTGATTT GGAAACCTTT 
GTCCCGCTGG TCTCGCCCCC TATTCATGCA TCGCTGAGTG AAGGCTTGTT TCACGCCTTT 
GATGAGAATC GGGACAATCA TATTGACTTT AAGGAGATAT CCTGTGGGCT CTCAGCTTGC 
TGTCGCGGCC CAGTAGCGGA GAGGCAGAAG TTTTGTTTTA AAGTGTTCGA CGTAGATCGT 
GATGGAGTTT TGTCCAGGGA CGAGATCCAC GAGATGGTGG TGGCGCTGCT GGAGGTGTGG 
AAGGATAACC GAACAGACAC TCTTCCTGAG TTTGATTCCA GTGTGTCAGA GATTGTGGAG 
GACATATTGA AAATGCACGA CACAACAAAA CAAGGACATT TAACTCTAGA GGACTATCAG 
ATATGGAGTG TGAAAAGTGC CCTCGCCAAT GAGTTCCTCA ACTTGCTGTT TCAGGTGTGT 
CATATTGTCC TGGGCCTGCG TCCTGCCACC CCTGAAGAGG AAGGCCAGAT CATTAGGGGT 
TGGTTGGAGA GGGAAAGCAG GCACGGTTTA CAGCCCAGTC AAAACTGGTT CCTGATCTCC 
ATGCAGTGGT GGCAGCAATG GAAAGACTAT GTCAGATATG ATAATAAGTC GATTGTCATG 
GAGCAGCCAT CAGTGCAGGG CAGCGGGATG AGAAGTTCTC AGTCTGCTGC ATCTGTAGAG 
CCTATCCTCA ACAGAATTGG ATTAGTGGTT CGCTCCCCCA GTTCCCCAGA AGAAAAATTC 
CCTGACAACA TCTCCAGCTC ATCAGAGGCT TCAGAGAGCA CTGGCCTAAC CCCACACATC 
AGCTCCTCTG CCACAGACGT GTGCTTTGCT CGGCAGCACA ACATCTCTGA CAACAACAAC 
CAGTGTTTCT CAAGCGCCAA TGGCCATTTG CCTAACAGCT TGGCCGTGCA GAGACCCGGT 
GCGATAGACA ACCAGCCTCT GGTGACCACC GAGCCCATGA AGGCACCCAC GCTGACTATG 
GAAGGAGGTC GACTGAAGAA GTCCCATCAA CTGTTGTCTG GCCGGGAGTT TGAGACGGTT 
CCAGAGCCGG TGTGGAGAGC ACTTTACCAC TGGTATGGGG CCAACCTTAG TCTGCCCCGA 
CCGGTTATCA AGTCTACAGT CAAGGCACAG CCTGAGCTAG AGCTCTTCCC CCTTTACCTT 
CTGTTCCTGC GACAGCAACC GGCCACACGG AGCCCTCAGT CCAACATCTG GGTCAATATG 
GGTAATGTTC CTTCTCCCAA CGCCCCGCTC AAGCGAGTGC TGGCATACAC AGGCTGTTTC 
AGCAGAATGT GCACCATTAA AGACATTCAC GAGTACCTGT CCCAGAGGTT ACGCATCAAA 
GAAGAGGACA TGCGCCTCTG GCTTTACAAC AGCGAGAATT ACCTCACTCT CCTGGACGAT 
GAAGATCACA CTTTAGAAAG CTTGAAAATT CCAGATGAAC AGCACTTAGT CATTGAGGTT 
CGAAACAAAG ATATGAGCTG GCCAGAGGAA ATGTCTTTCA TTGCAAACAG CAGCAAAATC 
GACAGACACA AAGTTCCTAC TGAGAAAGGT GCCACAGGTT TGAGTAATCT GGGCAACACG 
TGCTTCATGA ACTCCAGTAT TCAGTGTGTA AGCAATACCA AGCCCCTCAC TGAGTACTTC 
ATCTCAGGGC GACACCTTTA TGAGCTTAAT AGAACTAATC CAATAGGAAT GCGTGGTCAC 
ATGGCCAAAT GCTATGGTGA TTTGGTACAG GAACTATGGA GCGGCACTCA AAAGAATGTT 
GCGCCTTTGA AACTTAGGTG GACGATAGCA AAGTACGCGC CAAGATTCAA TGGCTTCCAG 
CAGCAAGACT CCCAGGAGCT TTTGGCCTTC CTGTTGGATG GGCTTCATGA AGACTTGAAT 
CGTGTGCATG AGAAGCCGTA TGTGGAGCTC AAGGACAGTG ATGGACGGCC GGACCATGAG 
GTTGCTACAG AGGCCTGGGA AAACCATTTG CGGAGAAATC GCTCTATCGT GGTGGACCTC 
TTCCATGGGC AGCTCAGATC CCAGGTGAAG TGCAAGACCT GTGGCCACAT AAGCGCACGC 
TTCGACCCCT TTAACTTCCT ATCGCTGCCT CTGCCAATGG ACAGCTCCAT GCACTTAGAA 
ATCACAGTCA TCAAACTTGA CGGCTCCACT CCAGTGCGAT ACGGGCTCCG TCTCAACATG 
GATGAGAAGT ACATGGGGCT AAAAAAACAG CTGAGCGAGC TCTGTAGCCT GAAACCTGAG 
CAGATCCTAC TGGCCGAAGT TCACACGTCC AACATCAAGA ACTTTCCTCT GGATAACCAG 
AAGGTGCGAC ACTCTGTCAA CGGCTTCCTC TGTGCGTTTG AGATCCCTGT GCCTGGTTCA 
CCCACTTCAG TCACTTCTCC TGTTCAAACA GAAAAACCAC CTATTGCCAA TGGGACAGCA 
GGAAACAATC ATATTGGAAA GTCTGGCCTG ATTCCTAATG GTATGCCCAG TACAGTGGTC 
CCCTGCACCC TTGAGAAACC CCTCGCCAAT GGTATCCCCA ATGGCCATGC TGTGCCAGTG 
CAGGACAGCC CATTCATTGG CTACATTATT GCCATGCACA GAAAGATGAT GCGGACGGAA 
CTGTACTTCC TGTCCTCTCA GAAGAACAGG CCCAGTCTGT TTGGTATGCC TCTGATTGTG 
CCATGCACAG TGCACACGAG TAAGAAGGAC CTGTATGATG CTGTGTGGAT TCAAGTGTCC 
CGACTTGCAA GTCCACTGCC GCCGCAGGAA GCCAGCAACC ATGCTCAAGA CTGTGATGAC 
AGTTTGGGCT ATCAGTACCC CTTTACGCTG CGGGTGGTGC AGAAAGATGG TAACTCCTGT 
GCCTGGTGTC CCTGGTACAG GTTCTGCAGA GGCTGCACAG TGGACTGTAA TGAAGACAGA 
GCATCTTTGG GGAATGCCTA CATTGCTGTA GACTGGGATC CCACCGCCTT GCACCTTCGT 
TACCAGACGT CTCAGGAAAG GATAGTTGAA GAGCATCCCA GTGTTGAGCA GAGTCGACGC 
GCTCAAGCAG AGCCCATCAG TCTGGACAGC TGTCTGAAAG CCTTCACCAG TGAAGAAGAG 
CTGGGAGAGG ACGAGCTCTA TTACTGCTCC AAGTGTAAAA CACACCGCCT GGCCACTAAG 
AAGCTGGACC TCTGGAGGCT GCCTCCTGTA CTGATTGTTC ATTTGAAACG CTTCCAATTT 
GTAAATGGTC GATGGATCAA GTCTCAGAAG ATTGTGAAGT TTCCACGCGA GAGCTTCGAC 
CCCAGTGCGT TCCTTGCCCC ACGGGAGGTG TCTCAGAGCC TTGGCAGCCT GCAGAGCCTG 
CAGAGCGAGT GCCTGGTGGA GGAGCTCCAG AGGCTGGAGA GAGGAGACTC CTCTGTGTCC 
TCCATCTCCA CCACCACCTT GGCCAGTCCT CCCATATCTG GCAGAGCCAC CCCGTGCTCC 
ATCAGGAGGG TCGGCAGTCC CCTGAGCAGA AACAGCAGTC CCGGCAGCAG CCCCAGAAAC 
ACAGGCCGAA AACAGGGCCG CTTGCGTCTT CCCCAGCTGG GCAGCAGACA TCGACTCTGC 
AACAGCAAAG AAAACCTGGA AGCCTGTAGC ACCAACAAGG AGAGCGAGAG CGAGGCCGAG 
CAAAGCGCAG TGACGGACGG GGAGAATCTG ACCCCTGATG CCAACACACA AGACACCTGG 
TCACTAGACG CCTCCAGCAA CGACTGCGAT GTCATTATGG TGAATGGACT AGGACAGGAC 
AGCAGTCAGA GCAATGGACA TTCAGAGGTC AGCGCCGATC TGGACACCCC TCACCAAAGA 
AATGACATCT GCCTGGAGTC CCTGTATAAT TTATATGCAA TCTCATGCCA TTCGGGAATA 
ATGGGAGGGG GGCACTATGT GACTTATGCC AAAAACCCCA ACGAAAAATG GTACTGTTAC 
AATGACAGCA GCTGCAAGGA AGTGCACTCG GACGAGATTG ACACAGATTC AGCCTACATC 
CTGTTTTATG AGCAACAGGG AGTGGACTAC TCGCAGTTTC TGCCAAAAAC GGAGGGCAAG 
AAGATGGCAG ACACCACTAG CATGGATGAA GACTTTGAAT CTGACTACAA GAAGTACTGT 
GTGCTGCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp32 ( XM_005157606.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005157663.1 Danio rerio ubiquitin specific peptidase 32 (usp32), transcript variant X2, mRNA.

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CloneID	ODa20508
Clone ID Related Accession (Same CDS sequence)	XM_005157606.3 , XM_005157606.4
Accession Version	XM_005157606.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4752bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ubiquitin carboxyl-terminal hydrolase 32 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005157606.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGCCA AAGAGTCACG GATAGGCTTC CTTTCGTATG ACGAGGCCGT TAAAAGAGTT 
ACCGATGTGG AGCTCAAACG TCTGCGAGAT GCCTTTAAGA GGACCAGTGG CCTGTCAGTG 
TATATGACCC AGCAATGCTT CTTCAGAGAA GTCCTGGGCG ACGGAGTGCC ACCAAAGGTT 
GCAGAAGTGA TCTACAACTC ATTTGGTGGC ACATCCAAAG GGCTACACTT CAACTATCTG 
ATCGTCGGCC TGGTTTTGTT AACAAGAGGG CGTGATGAAG AGAAAGCCAA ATACATCTTC 
AGCTTGTTCG CGAGTGATTC TGGCACCTTT GTGGTCCGGG AGGATGTGGA GAACATGCTG 
CGCTCAGTGG ATGGGGAGGT CCCGCCTTCT CTCAGGAAGT GCTTTACTGA GGGGGAGAAG 
GTGAATTATG AGAAGTTTAA GAGCTGGCTG CTTCAGAATA AAGATGCTTT CACGTTCTCC 
CGCTGGCTGC TGTCAGGTGG TGTGTGCGTA ACGTTGACTG ACGACAGCGA CACACCCACC 
TTCTATCAAA CCCTGGCAGG TGTCACTCAC TTAGAAGAAT CAGACATTAT CGACTTGGAA 
AAACGCTACT GGCTGCTGAA AGCTCAATCA AGGACAGGCC GCTTTGATTT GGAAACCTTT 
GTCCCGCTGG TCTCGCCCCC TATTCATGCA TCGCTGAGTG AAGGCTTGTT TCACGCCTTT 
GATGAGAATC GGGACAATCA TATTGACTTT AAGGAGATAT CCTGTGGGCT CTCAGCTTGC 
TGTCGCGGCC CAGTAGCGGA GAGGCAGAAG TTTTGTTTTA AAGTGTTCGA CGTAGATCGT 
GATGGAGTTT TGTCCAGGGA CGAGATCCAC GAGATGGTGG TGGCGCTGCT GGAGGTGTGG 
AAGGATAACC GAACAGACAC TCTTCCTGAG TTTGATTCCA GTGTGTCAGA GATTGTGGAG 
GACATATTGA AAATGCACGA CACAACAAAA CAAGGACATT TAACTCTAGA GGACTATCAG 
ATATGGAGTG TGAAAAGTGC CCTCGCCAAT GAGTTCCTCA ACTTGCTGTT TCAGGTGTGT 
CATATTGTCC TGGGCCTGCG TCCTGCCACC CCTGAAGAGG AAGGCCAGAT CATTAGGGGT 
TGGTTGGAGA GGGAAAGCAG GCACGGTTTA CAGCCCAGTC AAAACTGGTT CCTGATCTCC 
ATGCAGTGGT GGCAGCAATG GAAAGACTAT GTCAGATATG ATAATAAGTC GATTGTCATG 
GAGCAGCCAT CAGTGCAGGG CAGCGGGATG AGAAGTTCTC AGTCTGCTGC ATCTGTAGAG 
CCTATCCTCA ACAGAATTGG ATTAGTGGTT CGCTCCCCCA GTTCCCCAGA AGAAAAATTC 
CCTGACAACA TCTCCAGCTC ATCAGAGGCT TCAGAGAGCA CTGGCCTAAC CCCACACATC 
AGCTCCTCTG CCACAGACGT GTGCTTTGCT CGGCAGCACA ACATCTCTGA CAACAACAAC 
CAGTGTTTCT CAAGCGCCAA TGGCCATTTG CCTAACAGCT TGGCCGTGCA GAGACCCGGT 
GCGATAGACA ACCAGCCTCT GGTGACCACC GAGCCCATGA AGGCACCCAC GCTGACTATG 
GAAGGAGGTC GACTGAAGAA GTCCCATCAA CTGTTGTCTG GCCGGGAGTT TGAGACGGTT 
CCAGAGCCGG TGTGGAGAGC ACTTTACCAC TGGTATGGGG CCAACCTTAG TCTGCCCCGA 
CCGGTTATCA AGTCTACAGT CAAGGCACAG CCTGAGCTAG AGCTCTTCCC CCTTTACCTT 
CTGTTCCTGC GACAGCAACC GGCCACACGG AGCCCTCAGT CCAACATCTG GGTCAATATG 
GGTAATGTTC CTTCTCCCAA CGCCCCGCTC AAGCGAGTGC TGGCATACAC AGGCTGTTTC 
AGCAGAATGT GCACCATTAA AGACATTCAC GAGTACCTGT CCCAGAGGTT ACGCATCAAA 
GAAGAGGACA TGCGCCTCTG GCTTTACAAC AGCGAGAATT ACCTCACTCT CCTGGACGAT 
GAAGATCACA CTTTAGAAAG CTTGAAAATT CCAGATGAAC AGCACTTAGT CATTGAGGTT 
CGAAACAAAG ATATGAGCTG GCCAGAGGAA ATGTCTTTCA TTGCAAACAG CAGCAAAATC 
GACAGACACA AAGTTCCTAC TGAGAAAGGT GCCACAGGTT TGAGTAATCT GGGCAACACG 
TGCTTCATGA ACTCCAGTAT TCAGTGTGTA AGCAATACCA AGCCCCTCAC TGAGTACTTC 
ATCTCAGGGC GACACCTTTA TGAGCTTAAT AGAACTAATC CAATAGGAAT GCGTGGTCAC 
ATGGCCAAAT GCTATGGTGA TTTGGTACAG GAACTATGGA GCGGCACTCA AAAGAATGTT 
GCGCCTTTGA AACTTAGGTG GACGATAGCA AAGTACGCGC CAAGATTCAA TGGCTTCCAG 
CAGCAAGACT CCCAGGAGCT TTTGGCCTTC CTGTTGGATG GGCTTCATGA AGACTTGAAT 
CGTGTGCATG AGAAGCCGTA TGTGGAGCTC AAGGACAGTG ATGGACGGCC GGACCATGAG 
GTTGCTACAG AGGCCTGGGA AAACCATTTG CGGAGAAATC GCTCTATCGT GGTGGACCTC 
TTCCATGGGC AGCTCAGATC CCAGGTGAAG TGCAAGACCT GTGGCCACAT AAGCGCACGC 
TTCGACCCCT TTAACTTCCT ATCGCTGCCT CTGCCAATGG ACAGCTCCAT GCACTTAGAA 
ATCACAGTCA TCAAACTTGA CGGCTCCACT CCAGTGCGAT ACGGGCTCCG TCTCAACATG 
GATGAGAAGT ACATGGGGCT AAAAAAACAG CTGAGCGAGC TCTGTAGCCT GAAACCTGAG 
CAGATCCTAC TGGCCGAAGT TCACACGTCC AACATCAAGA ACTTTCCTCT GGATAACCAG 
AAGGTGCGAC ACTCTGTCAA CGGCTTCCTC TGTGCGTTTG AGATCCCTGT GCCTGGTTCA 
CCCACTTCAG TCACTTCTCC TGTTCAAACA GAAAAACCAC CTATTGCCAA TGGGACAGCA 
GGAAACAATC ATATTGGAAA GTCTGGCCTG ATTCCTAATG GTATGCCCAG TACAGTGGTC 
CCCTGCACCC TTGAGAAACC CCTCGCCAAT GGTATCCCCA ATGGCCATGC TGTGCCAGTG 
CAGGACAGCC CATTCATTGG CTACATTATT GCCATGCACA GAAAGATGAT GCGGACGGAA 
CTGTACTTCC TGTCCTCTCA GAAGAACAGG CCCAGTCTGT TTGGTATGCC TCTGATTGTG 
CCATGCACAG TGCACACGAG TAAGAAGGAC CTGTATGATG CTGTGTGGAT TCAAGTGTCC 
CGACTTGCAA GTCCACTGCC GCCGCAGGAA GCCAGCAACC ATGCTCAAGA CTGTGATGAC 
AGTTTGGGCT ATCAGTACCC CTTTACGCTG CGGGTGGTGC AGAAAGATGG TAACTCCTGT 
GCCTGGTGTC CCTGGTACAG GTTCTGCAGA GGCTGCACAG TGGACTGTAA TGAAGACAGA 
GCATCTTTGG GGAATGCCTA CATTGCTGTA GACTGGGATC CCACCGCCTT GCACCTTCGT 
TACCAGACGT CTCAGGAAAG GATAGTTGAA GAGCATCCCA GTGTTGAGCA GAGTCGACGC 
GCTCAAGCAG AGCCCATCAG TCTGGACAGC TGTCTGAAAG CCTTCACCAG TGAAGAAGAG 
CTGGGAGAGG ACGAGCTCTA TTACTGCTCC AAGTGTAAAA CACACCGCCT GGCCACTAAG 
AAGCTGGACC TCTGGAGGCT GCCTCCTGTA CTGATTGTTC ATTTGAAACG CTTCCAATTT 
GTAAATGGTC GATGGATCAA GTCTCAGAAG ATTGTGAAGT TTCCACGCGA GAGCTTCGAC 
CCCAGTGCGT TCCTTGCCCC ACGGGAGGTG TCTCAGAGCC TTGGCAGCCT GCAGAGCCTG 
CAGAGCGAGT GCCTGGTGGA GGAGCTCCAG AGGCTGGAGA GAGGAGACTC CTCTGTGTCC 
TCCATCTCCA CCACCACCTT GGCCAGTCCT CCCATATCTG GCAGAGCCAC CCCGTGCTCC 
ATCAGGAGGG TCGGCAGTCC CCTGAGCAGA AACAGCAGTC CCGGCAGCAG CCCCAGAAAC 
ACAGGCCGAA AACAGGGCCG CTTGCGTCTT CCCCAGCTGG GCAGCAGACA TCGACTCTGC 
AACAGCAAAG AAAACCTGGA AGCCTGTAGC ACCAACAAGG AGAGCGAGAG CGAGGCCGAG 
CAAAGCGCAG TGACGGACGG GGAGAATCTG ACCCCTGATG CCAACACACA AGACACCTGG 
TCACTAGACG CCTCCAGCAA CGACTGCGAT GTCATTATGG TGAATGGACT AGGACAGGAC 
AGCAGTCAGA GCAATGGACA TTCAGAGGTC AGCGCCGATC TGGACACCCC TCACCAAAGA 
AATGACATCT GCCTGGAGTC CCTGTATAAT TTATATGCAA TCTCATGCCA TTCGGGAATA 
ATGGGAGGGG GGCACTATGT GACTTATGCC AAAAACCCCA ACGAAAAATG GTACTGTTAC 
AATGACAGCA GCTGCAAGGA AGTGCACTCG GACGAGATTG ACACAGATTC AGCCTACATC 
CTGTTTTATG AGCAACAGGG AGTGGACTAC TCGCAGTTTC TGCCAAAAAC GGAGGGCAAG 
AAGATGGCAG ACACCACTAG CATGGATGAA GACTTTGAAT CTGACTACAA GAAGTACTGT 
GTGCTGCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005157663.1
CDS	148..4899
Translation	MGAKESRIGFLSYDEAVKRVTDVELKRLRDAFKRTSGLSVYMTQQCFFREVLGDGVPPKVAEVIYNSFGGTSKGLHFNYLIVGLVLLTRGRDEEKAKYIFSLFASDSGTFVVREDVENMLRSVDGEVPPSLRKCFTEGEKVNYEKFKSWLLQNKDAFTFSRWLLSGGVCVTLTDDSDTPTFYQTLAGVTHLEESDIIDLEKRYWLLKAQSRTGRFDLETFVPLVSPPIHASLSEGLFHAFDENRDNHIDFKEISCGLSACCRGPVAERQKFCFKVFDVDRDGVLSRDEIHEMVVALLEVWKDNRTDTLPEFDSSVSEIVEDILKMHDTTKQGHLTLEDYQIWSVKSALANEFLNLLFQVCHIVLGLRPATPEEEGQIIRGWLERESRHGLQPSQNWFLISMQWWQQWKDYVRYDNKSIVMEQPSVQGSGMRSSQSAASVEPILNRIGLVVRSPSSPEEKFPDNISSSSEASESTGLTPHISSSATDVCFARQHNISDNNNQCFSSANGHLPNSLAVQRPGAIDNQPLVTTEPMKAPTLTMEGGRLKKSHQLLSGREFETVPEPVWRALYHWYGANLSLPRPVIKSTVKAQPELELFPLYLLFLRQQPATRSPQSNIWVNMGNVPSPNAPLKRVLAYTGCFSRMCTIKDIHEYLSQRLRIKEEDMRLWLYNSENYLTLLDDEDHTLESLKIPDEQHLVIEVRNKDMSWPEEMSFIANSSKIDRHKVPTEKGATGLSNLGNTCFMNSSIQCVSNTKPLTEYFISGRHLYELNRTNPIGMRGHMAKCYGDLVQELWSGTQKNVAPLKLRWTIAKYAPRFNGFQQQDSQELLAFLLDGLHEDLNRVHEKPYVELKDSDGRPDHEVATEAWENHLRRNRSIVVDLFHGQLRSQVKCKTCGHISARFDPFNFLSLPLPMDSSMHLEITVIKLDGSTPVRYGLRLNMDEKYMGLKKQLSELCSLKPEQILLAEVHTSNIKNFPLDNQKVRHSVNGFLCAFEIPVPGSPTSVTSPVQTEKPPIANGTAGNNHIGKSGLIPNGMPSTVVPCTLEKPLANGIPNGHAVPVQDSPFIGYIIAMHRKMMRTELYFLSSQKNRPSLFGMPLIVPCTVHTSKKDLYDAVWIQVSRLASPLPPQEASNHAQDCDDSLGYQYPFTLRVVQKDGNSCAWCPWYRFCRGCTVDCNEDRASLGNAYIAVDWDPTALHLRYQTSQERIVEEHPSVEQSRRAQAEPISLDSCLKAFTSEEELGEDELYYCSKCKTHRLATKKLDLWRLPPVLIVHLKRFQFVNGRWIKSQKIVKFPRESFDPSAFLAPREVSQSLGSLQSLQSECLVEELQRLERGDSSVSSISTTTLASPPISGRATPCSIRRVGSPLSRNSSPGSSPRNTGRKQGRLRLPQLGSRHRLCNSKENLEACSTNKESESEAEQSAVTDGENLTPDANTQDTWSLDASSNDCDVIMVNGLGQDSSQSNGHSEVSADLDTPHQRNDICLESLYNLYAISCHSGIMGGGHYVTYAKNPNEKWYCYNDSSCKEVHSDEIDTDSAYILFYEQQGVDYSQFLPKTEGKKMADTTSMDEDFESDYKKYCVLQ

Target ORF information:

RefSeq Version	XM_005157606.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ubiquitin specific peptidase 32 (usp32), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005157606.4

ORF Insert Sequence:

ATGGGCGCCA AAGAGTCACG GATAGGCTTC CTTTCGTATG ACGAGGCCGT TAAAAGAGTT 
ACCGATGTGG AGCTCAAACG TCTGCGAGAT GCCTTTAAGA GGACCAGTGG CCTGTCAGTG 
TATATGACCC AGCAATGCTT CTTCAGAGAA GTCCTGGGCG ACGGAGTGCC ACCAAAGGTT 
GCAGAAGTGA TCTACAACTC ATTTGGTGGC ACATCCAAAG GGCTACACTT CAACTATCTG 
ATCGTCGGCC TGGTTTTGTT AACAAGAGGG CGTGATGAAG AGAAAGCCAA ATACATCTTC 
AGCTTGTTCG CGAGTGATTC TGGCACCTTT GTGGTCCGGG AGGATGTGGA GAACATGCTG 
CGCTCAGTGG ATGGGGAGGT CCCGCCTTCT CTCAGGAAGT GCTTTACTGA GGGGGAGAAG 
GTGAATTATG AGAAGTTTAA GAGCTGGCTG CTTCAGAATA AAGATGCTTT CACGTTCTCC 
CGCTGGCTGC TGTCAGGTGG TGTGTGCGTA ACGTTGACTG ACGACAGCGA CACACCCACC 
TTCTATCAAA CCCTGGCAGG TGTCACTCAC TTAGAAGAAT CAGACATTAT CGACTTGGAA 
AAACGCTACT GGCTGCTGAA AGCTCAATCA AGGACAGGCC GCTTTGATTT GGAAACCTTT 
GTCCCGCTGG TCTCGCCCCC TATTCATGCA TCGCTGAGTG AAGGCTTGTT TCACGCCTTT 
GATGAGAATC GGGACAATCA TATTGACTTT AAGGAGATAT CCTGTGGGCT CTCAGCTTGC 
TGTCGCGGCC CAGTAGCGGA GAGGCAGAAG TTTTGTTTTA AAGTGTTCGA CGTAGATCGT 
GATGGAGTTT TGTCCAGGGA CGAGATCCAC GAGATGGTGG TGGCGCTGCT GGAGGTGTGG 
AAGGATAACC GAACAGACAC TCTTCCTGAG TTTGATTCCA GTGTGTCAGA GATTGTGGAG 
GACATATTGA AAATGCACGA CACAACAAAA CAAGGACATT TAACTCTAGA GGACTATCAG 
ATATGGAGTG TGAAAAGTGC CCTCGCCAAT GAGTTCCTCA ACTTGCTGTT TCAGGTGTGT 
CATATTGTCC TGGGCCTGCG TCCTGCCACC CCTGAAGAGG AAGGCCAGAT CATTAGGGGT 
TGGTTGGAGA GGGAAAGCAG GCACGGTTTA CAGCCCAGTC AAAACTGGTT CCTGATCTCC 
ATGCAGTGGT GGCAGCAATG GAAAGACTAT GTCAGATATG ATAATAAGTC GATTGTCATG 
GAGCAGCCAT CAGTGCAGGG CAGCGGGATG AGAAGTTCTC AGTCTGCTGC ATCTGTAGAG 
CCTATCCTCA ACAGAATTGG ATTAGTGGTT CGCTCCCCCA GTTCCCCAGA AGAAAAATTC 
CCTGACAACA TCTCCAGCTC ATCAGAGGCT TCAGAGAGCA CTGGCCTAAC CCCACACATC 
AGCTCCTCTG CCACAGACGT GTGCTTTGCT CGGCAGCACA ACATCTCTGA CAACAACAAC 
CAGTGTTTCT CAAGCGCCAA TGGCCATTTG CCTAACAGCT TGGCCGTGCA GAGACCCGGT 
GCGATAGACA ACCAGCCTCT GGTGACCACC GAGCCCATGA AGGCACCCAC GCTGACTATG 
GAAGGAGGTC GACTGAAGAA GTCCCATCAA CTGTTGTCTG GCCGGGAGTT TGAGACGGTT 
CCAGAGCCGG TGTGGAGAGC ACTTTACCAC TGGTATGGGG CCAACCTTAG TCTGCCCCGA 
CCGGTTATCA AGTCTACAGT CAAGGCACAG CCTGAGCTAG AGCTCTTCCC CCTTTACCTT 
CTGTTCCTGC GACAGCAACC GGCCACACGG AGCCCTCAGT CCAACATCTG GGTCAATATG 
GGTAATGTTC CTTCTCCCAA CGCCCCGCTC AAGCGAGTGC TGGCATACAC AGGCTGTTTC 
AGCAGAATGT GCACCATTAA AGACATTCAC GAGTACCTGT CCCAGAGGTT ACGCATCAAA 
GAAGAGGACA TGCGCCTCTG GCTTTACAAC AGCGAGAATT ACCTCACTCT CCTGGACGAT 
GAAGATCACA CTTTAGAAAG CTTGAAAATT CCAGATGAAC AGCACTTAGT CATTGAGGTT 
CGAAACAAAG ATATGAGCTG GCCAGAGGAA ATGTCTTTCA TTGCAAACAG CAGCAAAATC 
GACAGACACA AAGTTCCTAC TGAGAAAGGT GCCACAGGTT TGAGTAATCT GGGCAACACG 
TGCTTCATGA ACTCCAGTAT TCAGTGTGTA AGCAATACCA AGCCCCTCAC TGAGTACTTC 
ATCTCAGGGC GACACCTTTA TGAGCTTAAT AGAACTAATC CAATAGGAAT GCGTGGTCAC 
ATGGCCAAAT GCTATGGTGA TTTGGTACAG GAACTATGGA GCGGCACTCA AAAGAATGTT 
GCGCCTTTGA AACTTAGGTG GACGATAGCA AAGTACGCGC CAAGATTCAA TGGCTTCCAG 
CAGCAAGACT CCCAGGAGCT TTTGGCCTTC CTGTTGGATG GGCTTCATGA AGACTTGAAT 
CGTGTGCATG AGAAGCCGTA TGTGGAGCTC AAGGACAGTG ATGGACGGCC GGACCATGAG 
GTTGCTACAG AGGCCTGGGA AAACCATTTG CGGAGAAATC GCTCTATCGT GGTGGACCTC 
TTCCATGGGC AGCTCAGATC CCAGGTGAAG TGCAAGACCT GTGGCCACAT AAGCGCACGC 
TTCGACCCCT TTAACTTCCT ATCGCTGCCT CTGCCAATGG ACAGCTCCAT GCACTTAGAA 
ATCACAGTCA TCAAACTTGA CGGCTCCACT CCAGTGCGAT ACGGGCTCCG TCTCAACATG 
GATGAGAAGT ACATGGGGCT AAAAAAACAG CTGAGCGAGC TCTGTAGCCT GAAACCTGAG 
CAGATCCTAC TGGCCGAAGT TCACACGTCC AACATCAAGA ACTTTCCTCT GGATAACCAG 
AAGGTGCGAC ACTCTGTCAA CGGCTTCCTC TGTGCGTTTG AGATCCCTGT GCCTGGTTCA 
CCCACTTCAG TCACTTCTCC TGTTCAAACA GAAAAACCAC CTATTGCCAA TGGGACAGCA 
GGAAACAATC ATATTGGAAA GTCTGGCCTG ATTCCTAATG GTATGCCCAG TACAGTGGTC 
CCCTGCACCC TTGAGAAACC CCTCGCCAAT GGTATCCCCA ATGGCCATGC TGTGCCAGTG 
CAGGACAGCC CATTCATTGG CTACATTATT GCCATGCACA GAAAGATGAT GCGGACGGAA 
CTGTACTTCC TGTCCTCTCA GAAGAACAGG CCCAGTCTGT TTGGTATGCC TCTGATTGTG 
CCATGCACAG TGCACACGAG TAAGAAGGAC CTGTATGATG CTGTGTGGAT TCAAGTGTCC 
CGACTTGCAA GTCCACTGCC GCCGCAGGAA GCCAGCAACC ATGCTCAAGA CTGTGATGAC 
AGTTTGGGCT ATCAGTACCC CTTTACGCTG CGGGTGGTGC AGAAAGATGG TAACTCCTGT 
GCCTGGTGTC CCTGGTACAG GTTCTGCAGA GGCTGCACAG TGGACTGTAA TGAAGACAGA 
GCATCTTTGG GGAATGCCTA CATTGCTGTA GACTGGGATC CCACCGCCTT GCACCTTCGT 
TACCAGACGT CTCAGGAAAG GATAGTTGAA GAGCATCCCA GTGTTGAGCA GAGTCGACGC 
GCTCAAGCAG AGCCCATCAG TCTGGACAGC TGTCTGAAAG CCTTCACCAG TGAAGAAGAG 
CTGGGAGAGG ACGAGCTCTA TTACTGCTCC AAGTGTAAAA CACACCGCCT GGCCACTAAG 
AAGCTGGACC TCTGGAGGCT GCCTCCTGTA CTGATTGTTC ATTTGAAACG CTTCCAATTT 
GTAAATGGTC GATGGATCAA GTCTCAGAAG ATTGTGAAGT TTCCACGCGA GAGCTTCGAC 
CCCAGTGCGT TCCTTGCCCC ACGGGAGGTG TCTCAGAGCC TTGGCAGCCT GCAGAGCCTG 
CAGAGCGAGT GCCTGGTGGA GGAGCTCCAG AGGCTGGAGA GAGGAGACTC CTCTGTGTCC 
TCCATCTCCA CCACCACCTT GGCCAGTCCT CCCATATCTG GCAGAGCCAC CCCGTGCTCC 
ATCAGGAGGG TCGGCAGTCC CCTGAGCAGA AACAGCAGTC CCGGCAGCAG CCCCAGAAAC 
ACAGGCCGAA AACAGGGCCG CTTGCGTCTT CCCCAGCTGG GCAGCAGACA TCGACTCTGC 
AACAGCAAAG AAAACCTGGA AGCCTGTAGC ACCAACAAGG AGAGCGAGAG CGAGGCCGAG 
CAAAGCGCAG TGACGGACGG GGAGAATCTG ACCCCTGATG CCAACACACA AGACACCTGG 
TCACTAGACG CCTCCAGCAA CGACTGCGAT GTCATTATGG TGAATGGACT AGGACAGGAC 
AGCAGTCAGA GCAATGGACA TTCAGAGGTC AGCGCCGATC TGGACACCCC TCACCAAAGA 
AATGACATCT GCCTGGAGTC CCTGTATAAT TTATATGCAA TCTCATGCCA TTCGGGAATA 
ATGGGAGGGG GGCACTATGT GACTTATGCC AAAAACCCCA ACGAAAAATG GTACTGTTAC 
AATGACAGCA GCTGCAAGGA AGTGCACTCG GACGAGATTG ACACAGATTC AGCCTACATC 
CTGTTTTATG AGCAACAGGG AGTGGACTAC TCGCAGTTTC TGCCAAAAAC GGAGGGCAAG 
AAGATGGCAG ACACCACTAG CATGGATGAA GACTTTGAAT CTGACTACAA GAAGTACTGT 
GTGCTGCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp32 ( XM_009291747.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009290022.1 Danio rerio ubiquitin specific peptidase 32 (usp32), transcript variant X3, mRNA.

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CloneID	ODa42710
Clone ID Related Accession (Same CDS sequence)	XM_009291747.2 , XM_009291747.3
Accession Version	XM_009291747.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4233bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ubiquitin carboxyl-terminal hydrolase 32 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009291747.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTATGGT TCTTCTGTTG CCACTCTGAT AGAGTCGCTC CGTTAGTAGA AGAATCAGAC 
ATTATCGACT TGGAAAAACG CTACTGGCTG CTGAAAGCTC AATCAAGGAC AGGCCGCTTT 
GATTTGGAAA CCTTTGTCCC GCTGGTCTCG CCCCCTATTC ATGCATCGCT GAGTGAAGGC 
TTGTTTCACG CCTTTGATGA GAATCGGGAC AATCATATTG ACTTTAAGGA GATATCCTGT 
GGGCTCTCAG CTTGCTGTCG CGGCCCAGTA GCGGAGAGGC AGAAGTTTTG TTTTAAAGTG 
TTCGACGTAG ATCGTGATGG AGTTTTGTCC AGGGACGAGA TCCACGAGAT GGTGGTGGCG 
CTGCTGGAGG TGTGGAAGGA TAACCGAACA GACACTCTTC CTGAGTTTGA TTCCAGTGTG 
TCAGAGATTG TGGAGGACAT ATTGAAAATG CACGACACAA CAAAACAAGG ACATTTAACT 
CTAGAGGACT ATCAGATATG GAGTGTGAAA AGTGCCCTCG CCAATGAGTT CCTCAACTTG 
CTGTTTCAGG TGTGTCATAT TGTCCTGGGC CTGCGTCCTG CCACCCCTGA AGAGGAAGGC 
CAGATCATTA GGGGTTGGTT GGAGAGGGAA AGCAGGCACG GTTTACAGCC CAGTCAAAAC 
TGGTTCCTGA TCTCCATGCA GTGGTGGCAG CAATGGAAAG ACTATGTCAG ATATGATAAT 
AAGTCGATTG TCATGGAGCA GCCATCAGTG CAGGGCAGCG GGATGAGAAG TTCTCAGTCT 
GCTGCATCTG TAGAGCCTAT CCTCAACAGA ATTGGATTAG TGGTTCGCTC CCCCAGTTCC 
CCAGAAGAAA AATTCCCTGA CAACATCTCC AGCTCATCAG AGGCTTCAGA GAGCACTGGT 
AATAGCCTAA CCCCACACAT CAGCTCCTCT GCCACAGACG TGTGCTTTGC TCGGCAGCAC 
AACATCTCTG ACAACAACAA CCAGTGTTTC TCAAGCGCCA ATGGCCATTT GCCTAACAGC 
TTGGCCGTGC AGAGACCCGG TGCGATAGAC AACCAGCCTC TGGTGACCAC CGAGCCCATG 
AAGGCACCCA CGCTGACTAT GGAAGGAGGT CGACTGAAGA AGTCCCATCA ACTGTTGTCT 
GGCCGGGAGT TTGAGACGGT TCCAGAGCCG GTGTGGAGAG CACTTTACCA CTGGTATGGG 
GCCAACCTTA GTCTGCCCCG ACCGGTTATC AAGTCTACAG TCAAGGCACA GCCTGAGCTA 
GAGCTCTTCC CCCTTTACCT TCTGTTCCTG CGACAGCAAC CGGCCACACG GAGCCCTCAG 
TCCAACATCT GGGTCAATAT GGGTAATGTT CCTTCTCCCA ACGCCCCGCT CAAGCGAGTG 
CTGGCATACA CAGGCTGTTT CAGCAGAATG TGCACCATTA AAGACATTCA CGAGTACCTG 
TCCCAGAGGT TACGCATCAA AGAAGAGGAC ATGCGCCTCT GGCTTTACAA CAGCGAGAAT 
TACCTCACTC TCCTGGACGA TGAAGATCAC ACTTTAGAAA GCTTGAAAAT TCCAGATGAA 
CAGCACTTAG TCATTGAGGT TCGAAACAAA GATATGAGCT GGCCAGAGGA AATGTCTTTC 
ATTGCAAACA GCAGCAAAAT CGACAGACAC AAAGTTCCTA CTGAGAAAGG TGCCACAGGT 
TTGAGTAATC TGGGCAACAC GTGCTTCATG AACTCCAGTA TTCAGTGTGT AAGCAATACC 
AAGCCCCTCA CTGAGTACTT CATCTCAGGG CGACACCTTT ATGAGCTTAA TAGAACTAAT 
CCAATAGGAA TGCGTGGTCA CATGGCCAAA TGCTATGGTG ATTTGGTACA GGAACTATGG 
AGCGGCACTC AAAAGAATGT TGCGCCTTTG AAACTTAGGT GGACGATAGC AAAGTACGCG 
CCAAGATTCA ATGGCTTCCA GCAGCAAGAC TCCCAGGAGC TTTTGGCCTT CCTGTTGGAT 
GGGCTTCATG AAGACTTGAA TCGTGTGCAT GAGAAGCCGT ATGTGGAGCT CAAGGACAGT 
GATGGACGGC CGGACCATGA GGTTGCTACA GAGGCCTGGG AAAACCATTT GCGGAGAAAT 
CGCTCTATCG TGGTGGACCT CTTCCATGGG CAGCTCAGAT CCCAGGTGAA GTGCAAGACC 
TGTGGCCACA TAAGCGCACG CTTCGACCCC TTTAACTTCC TATCGCTGCC TCTGCCAATG 
GACAGCTCCA TGCACTTAGA AATCACAGTC ATCAAACTTG ACGGCTCCAC TCCAGTGCGA 
TACGGGCTCC GTCTCAACAT GGATGAGAAG TACATGGGGC TAAAAAAACA GCTGAGCGAG 
CTCTGTAGCC TGAAACCTGA GCAGATCCTA CTGGCCGAAG TTCACACGTC CAACATCAAG 
AACTTTCCTC TGGATAACCA GAAGGTGCGA CACTCTGTCA ACGGCTTCCT CTGTGCGTTT 
GAGATCCCTG TGCCTGGTTC ACCCACTTCA GTCACTTCTC CTGTTCAAAC AGAAAAACCA 
CCTATTGCCA ATGGGACAGC AGGAAACAAT CATATTGGAA AGTCTGGCCT GATTCCTAAT 
GGTATGCCCA GTACAGTGGT CCCCTGCACC CTTGAGAAAC CCCTCGCCAA TGGTATCCCC 
AATGGCCATG CTGTGCCAGT GCAGGACAGC CCATTCATTG GCTACATTAT TGCCATGCAC 
AGAAAGATGA TGCGGACGGA ACTGTACTTC CTGTCCTCTC AGAAGAACAG GCCCAGTCTG 
TTTGGTATGC CTCTGATTGT GCCATGCACA GTGCACACGA GTAAGAAGGA CCTGTATGAT 
GCTGTGTGGA TTCAAGTGTC CCGACTTGCA AGTCCACTGC CGCCGCAGGA AGCCAGCAAC 
CATGCTCAAG ACTGTGATGA CAGTTTGGGC TATCAGTACC CCTTTACGCT GCGGGTGGTG 
CAGAAAGATG GTAACTCCTG TGCCTGGTGT CCCTGGTACA GGTTCTGCAG AGGCTGCACA 
GTGGACTGTA ATGAAGACAG AGCATCTTTG GGGAATGCCT ACATTGCTGT AGACTGGGAT 
CCCACCGCCT TGCACCTTCG TTACCAGACG TCTCAGGAAA GGATAGTTGA AGAGCATCCC 
AGTGTTGAGC AGAGTCGACG CGCTCAAGCA GAGCCCATCA GTCTGGACAG CTGTCTGAAA 
GCCTTCACCA GTGAAGAAGA GCTGGGAGAG GACGAGCTCT ATTACTGCTC CAAGTGTAAA 
ACACACCGCC TGGCCACTAA GAAGCTGGAC CTCTGGAGGC TGCCTCCTGT ACTGATTGTT 
CATTTGAAAC GCTTCCAATT TGTAAATGGT CGATGGATCA AGTCTCAGAA GATTGTGAAG 
TTTCCACGCG AGAGCTTCGA CCCCAGTGCG TTCCTTGCCC CACGGGAGGT GTCTCAGAGC 
CTTGGCAGCC TGCAGAGCCT GCAGAGCGAG TGCCTGGTGG AGGAGCTCCA GAGGCTGGAG 
AGAGGAGACT CCTCTGTGTC CTCCATCTCC ACCACCACCT TGGCCAGTCC TCCCATATCT 
GGCAGAGCCA CCCCGTGCTC CATCAGGAGG GTCGGCAGTC CCCTGAGCAG AAACAGCAGT 
CCCGGCAGCA GCCCCAGAAA CACAGGCCGA AAACAGGGCC GCTTGCGTCT TCCCCAGCTG 
GGCAGCAGAC ATCGACTCTG CAACAGCAAA GAAAACCTGG AAGCCTGTAG CACCAACAAG 
GAGAGCGAGA GCGAGGCCGA GCAAAGCGCA GTGACGGACG GGGAGAATCT GACCCCTGAT 
GCCAACACAC AAGACACCTG GTCACTAGAC GCCTCCAGCA ACGACTGCGA TGTCATTATG 
GTGAATGGAC TAGGACAGGA CAGCAGTCAG AGCAATGGAC ATTCAGAGGT CAGCGCCGAT 
CTGGACACCC CTCACCAAAG AAATGACATC TGCCTGGAGT CCCTGTATAA TTTATATGCA 
ATCTCATGCC ATTCGGGAAT AATGGGAGGG GGGCACTATG TGACTTATGC CAAAAACCCC 
AACGAAAAAT GGTACTGTTA CAATGACAGC AGCTGCAAGG AAGTGCACTC GGACGAGATT 
GACACAGATT CAGCCTACAT CCTGTTTTAT GAGCAACAGG GAGTGGACTA CTCGCAGTTT 
CTGCCAAAAA CGGAGGGCAA GAAGATGGCA GACACCACTA GCATGGATGA AGACTTTGAA 
TCTGACTACA AGAAGTACTG TGTGCTGCAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009290022.1
CDS	318..4550
Translation	MVWFFCCHSDRVAPLVEESDIIDLEKRYWLLKAQSRTGRFDLETFVPLVSPPIHASLSEGLFHAFDENRDNHIDFKEISCGLSACCRGPVAERQKFCFKVFDVDRDGVLSRDEIHEMVVALLEVWKDNRTDTLPEFDSSVSEIVEDILKMHDTTKQGHLTLEDYQIWSVKSALANEFLNLLFQVCHIVLGLRPATPEEEGQIIRGWLERESRHGLQPSQNWFLISMQWWQQWKDYVRYDNKSIVMEQPSVQGSGMRSSQSAASVEPILNRIGLVVRSPSSPEEKFPDNISSSSEASESTGNSLTPHISSSATDVCFARQHNISDNNNQCFSSANGHLPNSLAVQRPGAIDNQPLVTTEPMKAPTLTMEGGRLKKSHQLLSGREFETVPEPVWRALYHWYGANLSLPRPVIKSTVKAQPELELFPLYLLFLRQQPATRSPQSNIWVNMGNVPSPNAPLKRVLAYTGCFSRMCTIKDIHEYLSQRLRIKEEDMRLWLYNSENYLTLLDDEDHTLESLKIPDEQHLVIEVRNKDMSWPEEMSFIANSSKIDRHKVPTEKGATGLSNLGNTCFMNSSIQCVSNTKPLTEYFISGRHLYELNRTNPIGMRGHMAKCYGDLVQELWSGTQKNVAPLKLRWTIAKYAPRFNGFQQQDSQELLAFLLDGLHEDLNRVHEKPYVELKDSDGRPDHEVATEAWENHLRRNRSIVVDLFHGQLRSQVKCKTCGHISARFDPFNFLSLPLPMDSSMHLEITVIKLDGSTPVRYGLRLNMDEKYMGLKKQLSELCSLKPEQILLAEVHTSNIKNFPLDNQKVRHSVNGFLCAFEIPVPGSPTSVTSPVQTEKPPIANGTAGNNHIGKSGLIPNGMPSTVVPCTLEKPLANGIPNGHAVPVQDSPFIGYIIAMHRKMMRTELYFLSSQKNRPSLFGMPLIVPCTVHTSKKDLYDAVWIQVSRLASPLPPQEASNHAQDCDDSLGYQYPFTLRVVQKDGNSCAWCPWYRFCRGCTVDCNEDRASLGNAYIAVDWDPTALHLRYQTSQERIVEEHPSVEQSRRAQAEPISLDSCLKAFTSEEELGEDELYYCSKCKTHRLATKKLDLWRLPPVLIVHLKRFQFVNGRWIKSQKIVKFPRESFDPSAFLAPREVSQSLGSLQSLQSECLVEELQRLERGDSSVSSISTTTLASPPISGRATPCSIRRVGSPLSRNSSPGSSPRNTGRKQGRLRLPQLGSRHRLCNSKENLEACSTNKESESEAEQSAVTDGENLTPDANTQDTWSLDASSNDCDVIMVNGLGQDSSQSNGHSEVSADLDTPHQRNDICLESLYNLYAISCHSGIMGGGHYVTYAKNPNEKWYCYNDSSCKEVHSDEIDTDSAYILFYEQQGVDYSQFLPKTEGKKMADTTSMDEDFESDYKKYCVLQ

Target ORF information:

RefSeq Version	XM_009291747.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ubiquitin specific peptidase 32 (usp32), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009291747.2

ORF Insert Sequence:

ATGGTATGGT TCTTCTGTTG CCACTCTGAT AGAGTCGCTC CGTTAGTAGA AGAATCAGAC 
ATTATCGACT TGGAAAAACG CTACTGGCTG CTGAAAGCTC AATCAAGGAC AGGCCGCTTT 
GATTTGGAAA CCTTTGTCCC GCTGGTCTCG CCCCCTATTC ATGCATCGCT GAGTGAAGGC 
TTGTTTCACG CCTTTGATGA GAATCGGGAC AATCATATTG ACTTTAAGGA GATATCCTGT 
GGGCTCTCAG CTTGCTGTCG CGGCCCAGTA GCGGAGAGGC AGAAGTTTTG TTTTAAAGTG 
TTCGACGTAG ATCGTGATGG AGTTTTGTCC AGGGACGAGA TCCACGAGAT GGTGGTGGCG 
CTGCTGGAGG TGTGGAAGGA TAACCGAACA GACACTCTTC CTGAGTTTGA TTCCAGTGTG 
TCAGAGATTG TGGAGGACAT ATTGAAAATG CACGACACAA CAAAACAAGG ACATTTAACT 
CTAGAGGACT ATCAGATATG GAGTGTGAAA AGTGCCCTCG CCAATGAGTT CCTCAACTTG 
CTGTTTCAGG TGTGTCATAT TGTCCTGGGC CTGCGTCCTG CCACCCCTGA AGAGGAAGGC 
CAGATCATTA GGGGTTGGTT GGAGAGGGAA AGCAGGCACG GTTTACAGCC CAGTCAAAAC 
TGGTTCCTGA TCTCCATGCA GTGGTGGCAG CAATGGAAAG ACTATGTCAG ATATGATAAT 
AAGTCGATTG TCATGGAGCA GCCATCAGTG CAGGGCAGCG GGATGAGAAG TTCTCAGTCT 
GCTGCATCTG TAGAGCCTAT CCTCAACAGA ATTGGATTAG TGGTTCGCTC CCCCAGTTCC 
CCAGAAGAAA AATTCCCTGA CAACATCTCC AGCTCATCAG AGGCTTCAGA GAGCACTGGT 
AATAGCCTAA CCCCACACAT CAGCTCCTCT GCCACAGACG TGTGCTTTGC TCGGCAGCAC 
AACATCTCTG ACAACAACAA CCAGTGTTTC TCAAGCGCCA ATGGCCATTT GCCTAACAGC 
TTGGCCGTGC AGAGACCCGG TGCGATAGAC AACCAGCCTC TGGTGACCAC CGAGCCCATG 
AAGGCACCCA CGCTGACTAT GGAAGGAGGT CGACTGAAGA AGTCCCATCA ACTGTTGTCT 
GGCCGGGAGT TTGAGACGGT TCCAGAGCCG GTGTGGAGAG CACTTTACCA CTGGTATGGG 
GCCAACCTTA GTCTGCCCCG ACCGGTTATC AAGTCTACAG TCAAGGCACA GCCTGAGCTA 
GAGCTCTTCC CCCTTTACCT TCTGTTCCTG CGACAGCAAC CGGCCACACG GAGCCCTCAG 
TCCAACATCT GGGTCAATAT GGGTAATGTT CCTTCTCCCA ACGCCCCGCT CAAGCGAGTG 
CTGGCATACA CAGGCTGTTT CAGCAGAATG TGCACCATTA AAGACATTCA CGAGTACCTG 
TCCCAGAGGT TACGCATCAA AGAAGAGGAC ATGCGCCTCT GGCTTTACAA CAGCGAGAAT 
TACCTCACTC TCCTGGACGA TGAAGATCAC ACTTTAGAAA GCTTGAAAAT TCCAGATGAA 
CAGCACTTAG TCATTGAGGT TCGAAACAAA GATATGAGCT GGCCAGAGGA AATGTCTTTC 
ATTGCAAACA GCAGCAAAAT CGACAGACAC AAAGTTCCTA CTGAGAAAGG TGCCACAGGT 
TTGAGTAATC TGGGCAACAC GTGCTTCATG AACTCCAGTA TTCAGTGTGT AAGCAATACC 
AAGCCCCTCA CTGAGTACTT CATCTCAGGG CGACACCTTT ATGAGCTTAA TAGAACTAAT 
CCAATAGGAA TGCGTGGTCA CATGGCCAAA TGCTATGGTG ATTTGGTACA GGAACTATGG 
AGCGGCACTC AAAAGAATGT TGCGCCTTTG AAACTTAGGT GGACGATAGC AAAGTACGCG 
CCAAGATTCA ATGGCTTCCA GCAGCAAGAC TCCCAGGAGC TTTTGGCCTT CCTGTTGGAT 
GGGCTTCATG AAGACTTGAA TCGTGTGCAT GAGAAGCCGT ATGTGGAGCT CAAGGACAGT 
GATGGACGGC CGGACCATGA GGTTGCTACA GAGGCCTGGG AAAACCATTT GCGGAGAAAT 
CGCTCTATCG TGGTGGACCT CTTCCATGGG CAGCTCAGAT CCCAGGTGAA GTGCAAGACC 
TGTGGCCACA TAAGCGCACG CTTCGACCCC TTTAACTTCC TATCGCTGCC TCTGCCAATG 
GACAGCTCCA TGCACTTAGA AATCACAGTC ATCAAACTTG ACGGCTCCAC TCCAGTGCGA 
TACGGGCTCC GTCTCAACAT GGATGAGAAG TACATGGGGC TAAAAAAACA GCTGAGCGAG 
CTCTGTAGCC TGAAACCTGA GCAGATCCTA CTGGCCGAAG TTCACACGTC CAACATCAAG 
AACTTTCCTC TGGATAACCA GAAGGTGCGA CACTCTGTCA ACGGCTTCCT CTGTGCGTTT 
GAGATCCCTG TGCCTGGTTC ACCCACTTCA GTCACTTCTC CTGTTCAAAC AGAAAAACCA 
CCTATTGCCA ATGGGACAGC AGGAAACAAT CATATTGGAA AGTCTGGCCT GATTCCTAAT 
GGTATGCCCA GTACAGTGGT CCCCTGCACC CTTGAGAAAC CCCTCGCCAA TGGTATCCCC 
AATGGCCATG CTGTGCCAGT GCAGGACAGC CCATTCATTG GCTACATTAT TGCCATGCAC 
AGAAAGATGA TGCGGACGGA ACTGTACTTC CTGTCCTCTC AGAAGAACAG GCCCAGTCTG 
TTTGGTATGC CTCTGATTGT GCCATGCACA GTGCACACGA GTAAGAAGGA CCTGTATGAT 
GCTGTGTGGA TTCAAGTGTC CCGACTTGCA AGTCCACTGC CGCCGCAGGA AGCCAGCAAC 
CATGCTCAAG ACTGTGATGA CAGTTTGGGC TATCAGTACC CCTTTACGCT GCGGGTGGTG 
CAGAAAGATG GTAACTCCTG TGCCTGGTGT CCCTGGTACA GGTTCTGCAG AGGCTGCACA 
GTGGACTGTA ATGAAGACAG AGCATCTTTG GGGAATGCCT ACATTGCTGT AGACTGGGAT 
CCCACCGCCT TGCACCTTCG TTACCAGACG TCTCAGGAAA GGATAGTTGA AGAGCATCCC 
AGTGTTGAGC AGAGTCGACG CGCTCAAGCA GAGCCCATCA GTCTGGACAG CTGTCTGAAA 
GCCTTCACCA GTGAAGAAGA GCTGGGAGAG GACGAGCTCT ATTACTGCTC CAAGTGTAAA 
ACACACCGCC TGGCCACTAA GAAGCTGGAC CTCTGGAGGC TGCCTCCTGT ACTGATTGTT 
CATTTGAAAC GCTTCCAATT TGTAAATGGT CGATGGATCA AGTCTCAGAA GATTGTGAAG 
TTTCCACGCG AGAGCTTCGA CCCCAGTGCG TTCCTTGCCC CACGGGAGGT GTCTCAGAGC 
CTTGGCAGCC TGCAGAGCCT GCAGAGCGAG TGCCTGGTGG AGGAGCTCCA GAGGCTGGAG 
AGAGGAGACT CCTCTGTGTC CTCCATCTCC ACCACCACCT TGGCCAGTCC TCCCATATCT 
GGCAGAGCCA CCCCGTGCTC CATCAGGAGG GTCGGCAGTC CCCTGAGCAG AAACAGCAGT 
CCCGGCAGCA GCCCCAGAAA CACAGGCCGA AAACAGGGCC GCTTGCGTCT TCCCCAGCTG 
GGCAGCAGAC ATCGACTCTG CAACAGCAAA GAAAACCTGG AAGCCTGTAG CACCAACAAG 
GAGAGCGAGA GCGAGGCCGA GCAAAGCGCA GTGACGGACG GGGAGAATCT GACCCCTGAT 
GCCAACACAC AAGACACCTG GTCACTAGAC GCCTCCAGCA ACGACTGCGA TGTCATTATG 
GTGAATGGAC TAGGACAGGA CAGCAGTCAG AGCAATGGAC ATTCAGAGGT CAGCGCCGAT 
CTGGACACCC CTCACCAAAG AAATGACATC TGCCTGGAGT CCCTGTATAA TTTATATGCA 
ATCTCATGCC ATTCGGGAAT AATGGGAGGG GGGCACTATG TGACTTATGC CAAAAACCCC 
AACGAAAAAT GGTACTGTTA CAATGACAGC AGCTGCAAGG AAGTGCACTC GGACGAGATT 
GACACAGATT CAGCCTACAT CCTGTTTTAT GAGCAACAGG GAGTGGACTA CTCGCAGTTT 
CTGCCAAAAA CGGAGGGCAA GAAGATGGCA GACACCACTA GCATGGATGA AGACTTTGAA 
TCTGACTACA AGAAGTACTG TGTGCTGCAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp32 ( XM_009291747.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009290022.1 Danio rerio ubiquitin specific peptidase 32 (usp32), transcript variant X3, mRNA.

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CloneID	ODa42710
Clone ID Related Accession (Same CDS sequence)	XM_009291747.2 , XM_009291747.3
Accession Version	XM_009291747.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4233bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ubiquitin carboxyl-terminal hydrolase 32 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009291747.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTATGGT TCTTCTGTTG CCACTCTGAT AGAGTCGCTC CGTTAGTAGA AGAATCAGAC 
ATTATCGACT TGGAAAAACG CTACTGGCTG CTGAAAGCTC AATCAAGGAC AGGCCGCTTT 
GATTTGGAAA CCTTTGTCCC GCTGGTCTCG CCCCCTATTC ATGCATCGCT GAGTGAAGGC 
TTGTTTCACG CCTTTGATGA GAATCGGGAC AATCATATTG ACTTTAAGGA GATATCCTGT 
GGGCTCTCAG CTTGCTGTCG CGGCCCAGTA GCGGAGAGGC AGAAGTTTTG TTTTAAAGTG 
TTCGACGTAG ATCGTGATGG AGTTTTGTCC AGGGACGAGA TCCACGAGAT GGTGGTGGCG 
CTGCTGGAGG TGTGGAAGGA TAACCGAACA GACACTCTTC CTGAGTTTGA TTCCAGTGTG 
TCAGAGATTG TGGAGGACAT ATTGAAAATG CACGACACAA CAAAACAAGG ACATTTAACT 
CTAGAGGACT ATCAGATATG GAGTGTGAAA AGTGCCCTCG CCAATGAGTT CCTCAACTTG 
CTGTTTCAGG TGTGTCATAT TGTCCTGGGC CTGCGTCCTG CCACCCCTGA AGAGGAAGGC 
CAGATCATTA GGGGTTGGTT GGAGAGGGAA AGCAGGCACG GTTTACAGCC CAGTCAAAAC 
TGGTTCCTGA TCTCCATGCA GTGGTGGCAG CAATGGAAAG ACTATGTCAG ATATGATAAT 
AAGTCGATTG TCATGGAGCA GCCATCAGTG CAGGGCAGCG GGATGAGAAG TTCTCAGTCT 
GCTGCATCTG TAGAGCCTAT CCTCAACAGA ATTGGATTAG TGGTTCGCTC CCCCAGTTCC 
CCAGAAGAAA AATTCCCTGA CAACATCTCC AGCTCATCAG AGGCTTCAGA GAGCACTGGT 
AATAGCCTAA CCCCACACAT CAGCTCCTCT GCCACAGACG TGTGCTTTGC TCGGCAGCAC 
AACATCTCTG ACAACAACAA CCAGTGTTTC TCAAGCGCCA ATGGCCATTT GCCTAACAGC 
TTGGCCGTGC AGAGACCCGG TGCGATAGAC AACCAGCCTC TGGTGACCAC CGAGCCCATG 
AAGGCACCCA CGCTGACTAT GGAAGGAGGT CGACTGAAGA AGTCCCATCA ACTGTTGTCT 
GGCCGGGAGT TTGAGACGGT TCCAGAGCCG GTGTGGAGAG CACTTTACCA CTGGTATGGG 
GCCAACCTTA GTCTGCCCCG ACCGGTTATC AAGTCTACAG TCAAGGCACA GCCTGAGCTA 
GAGCTCTTCC CCCTTTACCT TCTGTTCCTG CGACAGCAAC CGGCCACACG GAGCCCTCAG 
TCCAACATCT GGGTCAATAT GGGTAATGTT CCTTCTCCCA ACGCCCCGCT CAAGCGAGTG 
CTGGCATACA CAGGCTGTTT CAGCAGAATG TGCACCATTA AAGACATTCA CGAGTACCTG 
TCCCAGAGGT TACGCATCAA AGAAGAGGAC ATGCGCCTCT GGCTTTACAA CAGCGAGAAT 
TACCTCACTC TCCTGGACGA TGAAGATCAC ACTTTAGAAA GCTTGAAAAT TCCAGATGAA 
CAGCACTTAG TCATTGAGGT TCGAAACAAA GATATGAGCT GGCCAGAGGA AATGTCTTTC 
ATTGCAAACA GCAGCAAAAT CGACAGACAC AAAGTTCCTA CTGAGAAAGG TGCCACAGGT 
TTGAGTAATC TGGGCAACAC GTGCTTCATG AACTCCAGTA TTCAGTGTGT AAGCAATACC 
AAGCCCCTCA CTGAGTACTT CATCTCAGGG CGACACCTTT ATGAGCTTAA TAGAACTAAT 
CCAATAGGAA TGCGTGGTCA CATGGCCAAA TGCTATGGTG ATTTGGTACA GGAACTATGG 
AGCGGCACTC AAAAGAATGT TGCGCCTTTG AAACTTAGGT GGACGATAGC AAAGTACGCG 
CCAAGATTCA ATGGCTTCCA GCAGCAAGAC TCCCAGGAGC TTTTGGCCTT CCTGTTGGAT 
GGGCTTCATG AAGACTTGAA TCGTGTGCAT GAGAAGCCGT ATGTGGAGCT CAAGGACAGT 
GATGGACGGC CGGACCATGA GGTTGCTACA GAGGCCTGGG AAAACCATTT GCGGAGAAAT 
CGCTCTATCG TGGTGGACCT CTTCCATGGG CAGCTCAGAT CCCAGGTGAA GTGCAAGACC 
TGTGGCCACA TAAGCGCACG CTTCGACCCC TTTAACTTCC TATCGCTGCC TCTGCCAATG 
GACAGCTCCA TGCACTTAGA AATCACAGTC ATCAAACTTG ACGGCTCCAC TCCAGTGCGA 
TACGGGCTCC GTCTCAACAT GGATGAGAAG TACATGGGGC TAAAAAAACA GCTGAGCGAG 
CTCTGTAGCC TGAAACCTGA GCAGATCCTA CTGGCCGAAG TTCACACGTC CAACATCAAG 
AACTTTCCTC TGGATAACCA GAAGGTGCGA CACTCTGTCA ACGGCTTCCT CTGTGCGTTT 
GAGATCCCTG TGCCTGGTTC ACCCACTTCA GTCACTTCTC CTGTTCAAAC AGAAAAACCA 
CCTATTGCCA ATGGGACAGC AGGAAACAAT CATATTGGAA AGTCTGGCCT GATTCCTAAT 
GGTATGCCCA GTACAGTGGT CCCCTGCACC CTTGAGAAAC CCCTCGCCAA TGGTATCCCC 
AATGGCCATG CTGTGCCAGT GCAGGACAGC CCATTCATTG GCTACATTAT TGCCATGCAC 
AGAAAGATGA TGCGGACGGA ACTGTACTTC CTGTCCTCTC AGAAGAACAG GCCCAGTCTG 
TTTGGTATGC CTCTGATTGT GCCATGCACA GTGCACACGA GTAAGAAGGA CCTGTATGAT 
GCTGTGTGGA TTCAAGTGTC CCGACTTGCA AGTCCACTGC CGCCGCAGGA AGCCAGCAAC 
CATGCTCAAG ACTGTGATGA CAGTTTGGGC TATCAGTACC CCTTTACGCT GCGGGTGGTG 
CAGAAAGATG GTAACTCCTG TGCCTGGTGT CCCTGGTACA GGTTCTGCAG AGGCTGCACA 
GTGGACTGTA ATGAAGACAG AGCATCTTTG GGGAATGCCT ACATTGCTGT AGACTGGGAT 
CCCACCGCCT TGCACCTTCG TTACCAGACG TCTCAGGAAA GGATAGTTGA AGAGCATCCC 
AGTGTTGAGC AGAGTCGACG CGCTCAAGCA GAGCCCATCA GTCTGGACAG CTGTCTGAAA 
GCCTTCACCA GTGAAGAAGA GCTGGGAGAG GACGAGCTCT ATTACTGCTC CAAGTGTAAA 
ACACACCGCC TGGCCACTAA GAAGCTGGAC CTCTGGAGGC TGCCTCCTGT ACTGATTGTT 
CATTTGAAAC GCTTCCAATT TGTAAATGGT CGATGGATCA AGTCTCAGAA GATTGTGAAG 
TTTCCACGCG AGAGCTTCGA CCCCAGTGCG TTCCTTGCCC CACGGGAGGT GTCTCAGAGC 
CTTGGCAGCC TGCAGAGCCT GCAGAGCGAG TGCCTGGTGG AGGAGCTCCA GAGGCTGGAG 
AGAGGAGACT CCTCTGTGTC CTCCATCTCC ACCACCACCT TGGCCAGTCC TCCCATATCT 
GGCAGAGCCA CCCCGTGCTC CATCAGGAGG GTCGGCAGTC CCCTGAGCAG AAACAGCAGT 
CCCGGCAGCA GCCCCAGAAA CACAGGCCGA AAACAGGGCC GCTTGCGTCT TCCCCAGCTG 
GGCAGCAGAC ATCGACTCTG CAACAGCAAA GAAAACCTGG AAGCCTGTAG CACCAACAAG 
GAGAGCGAGA GCGAGGCCGA GCAAAGCGCA GTGACGGACG GGGAGAATCT GACCCCTGAT 
GCCAACACAC AAGACACCTG GTCACTAGAC GCCTCCAGCA ACGACTGCGA TGTCATTATG 
GTGAATGGAC TAGGACAGGA CAGCAGTCAG AGCAATGGAC ATTCAGAGGT CAGCGCCGAT 
CTGGACACCC CTCACCAAAG AAATGACATC TGCCTGGAGT CCCTGTATAA TTTATATGCA 
ATCTCATGCC ATTCGGGAAT AATGGGAGGG GGGCACTATG TGACTTATGC CAAAAACCCC 
AACGAAAAAT GGTACTGTTA CAATGACAGC AGCTGCAAGG AAGTGCACTC GGACGAGATT 
GACACAGATT CAGCCTACAT CCTGTTTTAT GAGCAACAGG GAGTGGACTA CTCGCAGTTT 
CTGCCAAAAA CGGAGGGCAA GAAGATGGCA GACACCACTA GCATGGATGA AGACTTTGAA 
TCTGACTACA AGAAGTACTG TGTGCTGCAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009290022.1
CDS	314..4546
Translation	MVWFFCCHSDRVAPLVEESDIIDLEKRYWLLKAQSRTGRFDLETFVPLVSPPIHASLSEGLFHAFDENRDNHIDFKEISCGLSACCRGPVAERQKFCFKVFDVDRDGVLSRDEIHEMVVALLEVWKDNRTDTLPEFDSSVSEIVEDILKMHDTTKQGHLTLEDYQIWSVKSALANEFLNLLFQVCHIVLGLRPATPEEEGQIIRGWLERESRHGLQPSQNWFLISMQWWQQWKDYVRYDNKSIVMEQPSVQGSGMRSSQSAASVEPILNRIGLVVRSPSSPEEKFPDNISSSSEASESTGNSLTPHISSSATDVCFARQHNISDNNNQCFSSANGHLPNSLAVQRPGAIDNQPLVTTEPMKAPTLTMEGGRLKKSHQLLSGREFETVPEPVWRALYHWYGANLSLPRPVIKSTVKAQPELELFPLYLLFLRQQPATRSPQSNIWVNMGNVPSPNAPLKRVLAYTGCFSRMCTIKDIHEYLSQRLRIKEEDMRLWLYNSENYLTLLDDEDHTLESLKIPDEQHLVIEVRNKDMSWPEEMSFIANSSKIDRHKVPTEKGATGLSNLGNTCFMNSSIQCVSNTKPLTEYFISGRHLYELNRTNPIGMRGHMAKCYGDLVQELWSGTQKNVAPLKLRWTIAKYAPRFNGFQQQDSQELLAFLLDGLHEDLNRVHEKPYVELKDSDGRPDHEVATEAWENHLRRNRSIVVDLFHGQLRSQVKCKTCGHISARFDPFNFLSLPLPMDSSMHLEITVIKLDGSTPVRYGLRLNMDEKYMGLKKQLSELCSLKPEQILLAEVHTSNIKNFPLDNQKVRHSVNGFLCAFEIPVPGSPTSVTSPVQTEKPPIANGTAGNNHIGKSGLIPNGMPSTVVPCTLEKPLANGIPNGHAVPVQDSPFIGYIIAMHRKMMRTELYFLSSQKNRPSLFGMPLIVPCTVHTSKKDLYDAVWIQVSRLASPLPPQEASNHAQDCDDSLGYQYPFTLRVVQKDGNSCAWCPWYRFCRGCTVDCNEDRASLGNAYIAVDWDPTALHLRYQTSQERIVEEHPSVEQSRRAQAEPISLDSCLKAFTSEEELGEDELYYCSKCKTHRLATKKLDLWRLPPVLIVHLKRFQFVNGRWIKSQKIVKFPRESFDPSAFLAPREVSQSLGSLQSLQSECLVEELQRLERGDSSVSSISTTTLASPPISGRATPCSIRRVGSPLSRNSSPGSSPRNTGRKQGRLRLPQLGSRHRLCNSKENLEACSTNKESESEAEQSAVTDGENLTPDANTQDTWSLDASSNDCDVIMVNGLGQDSSQSNGHSEVSADLDTPHQRNDICLESLYNLYAISCHSGIMGGGHYVTYAKNPNEKWYCYNDSSCKEVHSDEIDTDSAYILFYEQQGVDYSQFLPKTEGKKMADTTSMDEDFESDYKKYCVLQ

Target ORF information:

RefSeq Version	XM_009291747.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ubiquitin specific peptidase 32 (usp32), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009291747.3

ORF Insert Sequence:

ATGGTATGGT TCTTCTGTTG CCACTCTGAT AGAGTCGCTC CGTTAGTAGA AGAATCAGAC 
ATTATCGACT TGGAAAAACG CTACTGGCTG CTGAAAGCTC AATCAAGGAC AGGCCGCTTT 
GATTTGGAAA CCTTTGTCCC GCTGGTCTCG CCCCCTATTC ATGCATCGCT GAGTGAAGGC 
TTGTTTCACG CCTTTGATGA GAATCGGGAC AATCATATTG ACTTTAAGGA GATATCCTGT 
GGGCTCTCAG CTTGCTGTCG CGGCCCAGTA GCGGAGAGGC AGAAGTTTTG TTTTAAAGTG 
TTCGACGTAG ATCGTGATGG AGTTTTGTCC AGGGACGAGA TCCACGAGAT GGTGGTGGCG 
CTGCTGGAGG TGTGGAAGGA TAACCGAACA GACACTCTTC CTGAGTTTGA TTCCAGTGTG 
TCAGAGATTG TGGAGGACAT ATTGAAAATG CACGACACAA CAAAACAAGG ACATTTAACT 
CTAGAGGACT ATCAGATATG GAGTGTGAAA AGTGCCCTCG CCAATGAGTT CCTCAACTTG 
CTGTTTCAGG TGTGTCATAT TGTCCTGGGC CTGCGTCCTG CCACCCCTGA AGAGGAAGGC 
CAGATCATTA GGGGTTGGTT GGAGAGGGAA AGCAGGCACG GTTTACAGCC CAGTCAAAAC 
TGGTTCCTGA TCTCCATGCA GTGGTGGCAG CAATGGAAAG ACTATGTCAG ATATGATAAT 
AAGTCGATTG TCATGGAGCA GCCATCAGTG CAGGGCAGCG GGATGAGAAG TTCTCAGTCT 
GCTGCATCTG TAGAGCCTAT CCTCAACAGA ATTGGATTAG TGGTTCGCTC CCCCAGTTCC 
CCAGAAGAAA AATTCCCTGA CAACATCTCC AGCTCATCAG AGGCTTCAGA GAGCACTGGT 
AATAGCCTAA CCCCACACAT CAGCTCCTCT GCCACAGACG TGTGCTTTGC TCGGCAGCAC 
AACATCTCTG ACAACAACAA CCAGTGTTTC TCAAGCGCCA ATGGCCATTT GCCTAACAGC 
TTGGCCGTGC AGAGACCCGG TGCGATAGAC AACCAGCCTC TGGTGACCAC CGAGCCCATG 
AAGGCACCCA CGCTGACTAT GGAAGGAGGT CGACTGAAGA AGTCCCATCA ACTGTTGTCT 
GGCCGGGAGT TTGAGACGGT TCCAGAGCCG GTGTGGAGAG CACTTTACCA CTGGTATGGG 
GCCAACCTTA GTCTGCCCCG ACCGGTTATC AAGTCTACAG TCAAGGCACA GCCTGAGCTA 
GAGCTCTTCC CCCTTTACCT TCTGTTCCTG CGACAGCAAC CGGCCACACG GAGCCCTCAG 
TCCAACATCT GGGTCAATAT GGGTAATGTT CCTTCTCCCA ACGCCCCGCT CAAGCGAGTG 
CTGGCATACA CAGGCTGTTT CAGCAGAATG TGCACCATTA AAGACATTCA CGAGTACCTG 
TCCCAGAGGT TACGCATCAA AGAAGAGGAC ATGCGCCTCT GGCTTTACAA CAGCGAGAAT 
TACCTCACTC TCCTGGACGA TGAAGATCAC ACTTTAGAAA GCTTGAAAAT TCCAGATGAA 
CAGCACTTAG TCATTGAGGT TCGAAACAAA GATATGAGCT GGCCAGAGGA AATGTCTTTC 
ATTGCAAACA GCAGCAAAAT CGACAGACAC AAAGTTCCTA CTGAGAAAGG TGCCACAGGT 
TTGAGTAATC TGGGCAACAC GTGCTTCATG AACTCCAGTA TTCAGTGTGT AAGCAATACC 
AAGCCCCTCA CTGAGTACTT CATCTCAGGG CGACACCTTT ATGAGCTTAA TAGAACTAAT 
CCAATAGGAA TGCGTGGTCA CATGGCCAAA TGCTATGGTG ATTTGGTACA GGAACTATGG 
AGCGGCACTC AAAAGAATGT TGCGCCTTTG AAACTTAGGT GGACGATAGC AAAGTACGCG 
CCAAGATTCA ATGGCTTCCA GCAGCAAGAC TCCCAGGAGC TTTTGGCCTT CCTGTTGGAT 
GGGCTTCATG AAGACTTGAA TCGTGTGCAT GAGAAGCCGT ATGTGGAGCT CAAGGACAGT 
GATGGACGGC CGGACCATGA GGTTGCTACA GAGGCCTGGG AAAACCATTT GCGGAGAAAT 
CGCTCTATCG TGGTGGACCT CTTCCATGGG CAGCTCAGAT CCCAGGTGAA GTGCAAGACC 
TGTGGCCACA TAAGCGCACG CTTCGACCCC TTTAACTTCC TATCGCTGCC TCTGCCAATG 
GACAGCTCCA TGCACTTAGA AATCACAGTC ATCAAACTTG ACGGCTCCAC TCCAGTGCGA 
TACGGGCTCC GTCTCAACAT GGATGAGAAG TACATGGGGC TAAAAAAACA GCTGAGCGAG 
CTCTGTAGCC TGAAACCTGA GCAGATCCTA CTGGCCGAAG TTCACACGTC CAACATCAAG 
AACTTTCCTC TGGATAACCA GAAGGTGCGA CACTCTGTCA ACGGCTTCCT CTGTGCGTTT 
GAGATCCCTG TGCCTGGTTC ACCCACTTCA GTCACTTCTC CTGTTCAAAC AGAAAAACCA 
CCTATTGCCA ATGGGACAGC AGGAAACAAT CATATTGGAA AGTCTGGCCT GATTCCTAAT 
GGTATGCCCA GTACAGTGGT CCCCTGCACC CTTGAGAAAC CCCTCGCCAA TGGTATCCCC 
AATGGCCATG CTGTGCCAGT GCAGGACAGC CCATTCATTG GCTACATTAT TGCCATGCAC 
AGAAAGATGA TGCGGACGGA ACTGTACTTC CTGTCCTCTC AGAAGAACAG GCCCAGTCTG 
TTTGGTATGC CTCTGATTGT GCCATGCACA GTGCACACGA GTAAGAAGGA CCTGTATGAT 
GCTGTGTGGA TTCAAGTGTC CCGACTTGCA AGTCCACTGC CGCCGCAGGA AGCCAGCAAC 
CATGCTCAAG ACTGTGATGA CAGTTTGGGC TATCAGTACC CCTTTACGCT GCGGGTGGTG 
CAGAAAGATG GTAACTCCTG TGCCTGGTGT CCCTGGTACA GGTTCTGCAG AGGCTGCACA 
GTGGACTGTA ATGAAGACAG AGCATCTTTG GGGAATGCCT ACATTGCTGT AGACTGGGAT 
CCCACCGCCT TGCACCTTCG TTACCAGACG TCTCAGGAAA GGATAGTTGA AGAGCATCCC 
AGTGTTGAGC AGAGTCGACG CGCTCAAGCA GAGCCCATCA GTCTGGACAG CTGTCTGAAA 
GCCTTCACCA GTGAAGAAGA GCTGGGAGAG GACGAGCTCT ATTACTGCTC CAAGTGTAAA 
ACACACCGCC TGGCCACTAA GAAGCTGGAC CTCTGGAGGC TGCCTCCTGT ACTGATTGTT 
CATTTGAAAC GCTTCCAATT TGTAAATGGT CGATGGATCA AGTCTCAGAA GATTGTGAAG 
TTTCCACGCG AGAGCTTCGA CCCCAGTGCG TTCCTTGCCC CACGGGAGGT GTCTCAGAGC 
CTTGGCAGCC TGCAGAGCCT GCAGAGCGAG TGCCTGGTGG AGGAGCTCCA GAGGCTGGAG 
AGAGGAGACT CCTCTGTGTC CTCCATCTCC ACCACCACCT TGGCCAGTCC TCCCATATCT 
GGCAGAGCCA CCCCGTGCTC CATCAGGAGG GTCGGCAGTC CCCTGAGCAG AAACAGCAGT 
CCCGGCAGCA GCCCCAGAAA CACAGGCCGA AAACAGGGCC GCTTGCGTCT TCCCCAGCTG 
GGCAGCAGAC ATCGACTCTG CAACAGCAAA GAAAACCTGG AAGCCTGTAG CACCAACAAG 
GAGAGCGAGA GCGAGGCCGA GCAAAGCGCA GTGACGGACG GGGAGAATCT GACCCCTGAT 
GCCAACACAC AAGACACCTG GTCACTAGAC GCCTCCAGCA ACGACTGCGA TGTCATTATG 
GTGAATGGAC TAGGACAGGA CAGCAGTCAG AGCAATGGAC ATTCAGAGGT CAGCGCCGAT 
CTGGACACCC CTCACCAAAG AAATGACATC TGCCTGGAGT CCCTGTATAA TTTATATGCA 
ATCTCATGCC ATTCGGGAAT AATGGGAGGG GGGCACTATG TGACTTATGC CAAAAACCCC 
AACGAAAAAT GGTACTGTTA CAATGACAGC AGCTGCAAGG AAGTGCACTC GGACGAGATT 
GACACAGATT CAGCCTACAT CCTGTTTTAT GAGCAACAGG GAGTGGACTA CTCGCAGTTT 
CTGCCAAAAA CGGAGGGCAA GAAGATGGCA GACACCACTA GCATGGATGA AGACTTTGAA 
TCTGACTACA AGAAGTACTG TGTGCTGCAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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usp32 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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