Usp32 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Usp32
Entrez Gene ID	237898
Full Name	ubiquitin specific peptidase 32
Synonyms	2900074J03Rik,6430526O11Rik,AW045245
General protein information	Preferred Names ubiquitin specific peptidase 32 Names ubiquitin carboxyl-terminal hydrolase 32 ubiquitin specific protease 32
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11\|11 C

mRNA and Protein(s)

mRNA	Protein	Name
XM_030245969.1	XP_030101829.1	ubiquitin carboxyl-terminal hydrolase 32 isoform X2
XM_030245970.1	XP_030101830.1	ubiquitin carboxyl-terminal hydrolase 32 isoform X3
XM_011248991.1	XP_011247293.1	ubiquitin carboxyl-terminal hydrolase 32 isoform X4
NM_001029934.1	NP_001025105.1	ubiquitin carboxyl-terminal hydrolase 32
XM_011248990.1	XP_011247292.1	ubiquitin carboxyl-terminal hydrolase 32 isoform X1

Pathways from BioSystems

Homologies

	LOC716857	XP_002808172.1
Canis lupus familiaris (dog)	USP32	XP_005624819.1
Bos taurus (cattle)	USP32	XP_002695689.2
Gallus gallus (chicken)	USP32	XP_004946797.1
Drosophila melanogaster (fruit fly)	CG8334	NP_001262075.1
Caenorhabditis elegans (roundworm)	cyk-3	NP_001022992.1
Xenopus tropicalis (tropical clawed frog)	usp32	XP_002933869.1
Mus musculus (house mouse)	Usp32	NP_001025105.1
Rattus norvegicus (Norway rat)	Usp32	NP_001100502.1
Danio rerio (zebrafish)	usp32	XP_686236.6
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001209	XP_321947.5
Homo sapiens (human)	USP32	NP_115971.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene-trap mutagenesis using Mol/MSM-1 embryonic stem cells from MSM/Ms mice.
Nakahara M, Tateyama H, Araki M, Nakagata N, Yamamura K, Araki K
Mammalian genome : official journal of the International Mammalian Genome Society24(5-6)228-39(2013 Jun)

USP32 is an active, membrane-bound ubiquitin protease overexpressed in breast cancers.
Akhavantabasi S, Akman HB, Sapmaz A, Keller J, Petty EM, Erson AE
Mammalian genome : official journal of the International Mammalian Genome Society21(7-8)388-97(2010 Aug)

Characterization of an exchangeable gene trap using pU-17 carrying a stop codon-beta geo cassette.
Taniwaki T, Haruna K, Nakamura H, Sekimoto T, Oike Y, Imaizumi T, Saito F, Muta M, Soejima Y, Utoh A, Nakagata N, Araki M, Yamamura K, Araki K
Development, growth & differentiation47(3)163-72(2005 Apr)

Identification and developmental analysis of genes expressed by dopaminergic neurons of the substantia nigra pars compacta.
Thuret S, Bhatt L, O'Leary DD, Simon HH
Molecular and cellular neurosciences25(3)394-405(2004 Mar)

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Stryke D, Kawamoto M, Huang CC, Johns SJ, King LA, Harper CA, Meng EC, Lee RE, Yee A, L'Italien L, Chuang PT, Young SG, Skarnes WC, Babbitt PC, Ferrin TE
Nucleic acids research31(1)278-81(2003 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Usp32 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Usp32 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu73889	XM_011248991.1 Latest version!	Mus musculus ubiquitin specific peptidase 32 (Usp32), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu85780	XM_030245969.1 Latest version!	Mus musculus ubiquitin specific peptidase 32 (Usp32), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu85781	XM_030245970.1 Latest version!	Mus musculus ubiquitin specific peptidase 32 (Usp32), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu11470	NM_001029934.1 Latest version!	Mus musculus ubiquitin specific peptidase 32 (Usp32), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$944.30 $1349.00
OMu73888	XM_011248990.1 Latest version!	Mus musculus ubiquitin specific peptidase 32 (Usp32), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Usp32 ( XM_011248991.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011247293.1 Mus musculus ubiquitin specific peptidase 32 (Usp32), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu73889
Clone ID Related Accession (Same CDS sequence)	XM_011248991.1
Accession Version	XM_011248991.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2742bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ubiquitin carboxyl-terminal hydrolase 32 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGCCA AGGAGTCACG GATCGGATTC CTCAGCTACG AGGAGGCGCT GAGGAGAGTT 
ACAGATGTTG AGCTGAAACG ACTCAAAGAT GCCTTCAAGA GGACCTGCGG CCTCTCCTAT 
TACATGAGTC AGCACTGCTT CATCAGGGAA GTGCTTGGGG ATGGAGTACC TCCTAAAGTT 
GCTGAGGTGA TTTACTGTTC TTTTGGTGGA ACATCCAAAG GGCTGCATTT CAATAACTTA 
ATAGTTGGAC TCGTCCTCCT TACAAGAGGC AAAGATGAAG AGAAAGCAAA ATATATTTTT 
AGTCTTTTTT CAAGTGAATC CGGGAGCTAT GTTGTACGGG AAGAAATGGA GAGAATGCTC 
CATGTGGTGG ATGGTAAAGT CCCAGATACA CTCAGGAAGT GTTTCTCAGA GGGTGAAAAG 
GTAAACTATG AAAAATTCAG AAATTGGCTT CTTCTAAACA AAGATGCTTT TACCTTCTCT 
CGCTGGCTGT TGTCTGGGGG TGTATATGTG ACCCTCACTG ATGACAGTGA CACTCCTACT 
TTCTACCAAA CTCTGGCTGG AGTCACACAT TTGGAAGAGT CAGACATCAT CGATCTGGAG 
AAACGGTATT GGCTACTGAA GGCCCAATCC CGAACTGGAC GTTTTGACTT AGAGACCTTT 
GGTCCATTGG TTTCACCACC TATTCGCCCT TCTCTGAGTG AAGGATTATT TAATGCTTTT 
GATGAAAATC GTGACAATCA CATAGACTTC AAGGAGATAT CCTGTGGGTT ATCAGCCTGC 
TGCAGGGGCC CCCTGGCTGA AAGACAAAAA TTTTGCTTCA AGGTGTTTGA TGTTGACCGT 
GATGGAGTTC TCTCCAGGGT TGAACTGAAA GACATGGTAG TTGCACTTTT GGAGGTCTGG 
AAAGATAACC GCACTGATGA TATTCCTGAA TTGCATATGG ATTTGTCAGA CATTGTAGAA 
AGGATATTGA ATGCACATGA TACCACAAAG GTGGGCCATC TTACTCTTGA AGACTATCAG 
ATATGGAGTG TGAAAAATGT TCTTGCCAAT GAGTTTTTGA ATCTCCTCTT CCAGGTATGC 
CACATAGTTC TGGGGTTAAG ACCAGCTACT CCAGAAGAAG AAGGACAAAT TATTAGAGGA 
TGGTTGGAAC GAGAAAGCAG GTATGGTCTG CAGCCAGGAC ACAATTGGTT TATCATCTCC 
ATGCAGTGGT GGCAACAGTG GAAAGAATAT GTCAAATATG ATGCCAGCCC TGTTGTAATT 
GAGCCATCAT CTGTTCTAAA TGGAGGAAAA TTTTCATTTG GAACAGCAGC ACATCCTATA 
GAGCATGGAG AAGATAGAAT CAGTAATAAC CTTGGTTATA TGAATACCAC AGAAGAGAAA 
TACTCAGACA ACATTTCTTC TGCATCTGAA GCCTCAGAGT CTACTGGCAG TGGCTTTCTG 
TATTCTGGCA CACCAGGGGC AGATATGTGC TTTGCAAGGC AGCATAACAC TTCTGACAAT 
AACAACCAGT GTTTACTAGG AGCCAATGGG AATATCCTTT TGCACCTTAA TCCTCAGAAG 
CCAGGAGCAA TTGATAACCA GCCACTAGTA ACTCAGGAAC CAGTAAAGGC TACATCATTA 
ACACTAGAAG GAGGGCGATT AAAACGAACT CCACAGCTAA TCCATGGAAG AGATTATGAA 
ATGGTCCCAG AACCCGTGTG GAGAGCACTT TATCATTGGT ATGGATCAAA CCTGGCCCTT 
CCTAGACCAG TAATCAAGAA TAGCAAAACG GACATCCCAG AACTAGAATT ATTTCCTCGC 
TATCTTCTCT TCCTTCGACA GCAGCCTGCC ACACGGACAC AGCAATCTAA CATCTGGGTG 
AATATGGGTA TGATGAGCCT GAGAATGTTT CCTCAGCATT TGCCTAGAGG AAATGTACCC 
TCTCCAAATG CGCCTCTAAA GCGAGTGTTA GCCTATACAG GCTGTTTTAG TAGAATGCAA 
ACCATCAAGG AAATTCATGA ATATCTGTCT CAAAGGCTTC GCATTAAAGA GGAAGATATG 
CGGCTGTGGC TGTACAATAG TGAGAACTAC CTGACTCTTC TGGATGATGA GGATCACAGA 
TTGGAATATT TGAAAATCCA GGATGAGCAG CACCTGGTAA TTGAAGTTCG AAACAAAGAT 
ATGAGTTGGC CTGAAGAAAT GTCTTTTATA GCAAACAGTA GCAAAATAGA TAGACATAAA 
GTTCCCACAG AAAAGGGCGC CACAGGTCTG AGTAACCTGG GAAACACATG CTTCATGAAC 
TCAAGCATCC AGTGTGTCAG CAATACACAA CCACTGACAC AGTACTTTAT CTCAGGGAGG 
CATCTTTATG AACTCAACAG GACAAATCCC ATTGGTATGA AAGGCCATAT GGCTAAATGT 
TATGGCGATT TGGTGCAAGA ACTTTGGAGT GGAACTCAGA AGAATGTTGC CCCCTTAAAG 
CTCCGGTGGA CCATAGCAAA ATATGCTCCC AGGTTTAATG GATTCCAGCA ACAAGACTCC 
CAAGAACTTC TGGCTTTCCT CTTGGATGGT CTTCATGAAG ATCTCAACCG AGTCCACGAG 
AAACCATATG TAGAACTAAA AGACAGTGAC GGCCGACCCG ACTGGGAAGT AGCTGCAGAG 
GCCTGGGACA ACCATCTAAG GAGAAATAGA TCAATTGTTG TACCACAAAT ATATGTGGCA 
CAATTTAAAT TTGAACAAAA GTGTTCTGTG GTTAGAGTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011247293.1
CDS	275..3016
Translation	MGAKESRIGFLSYEEALRRVTDVELKRLKDAFKRTCGLSYYMSQHCFIREVLGDGVPPKVAEVIYCSFGGTSKGLHFNNLIVGLVLLTRGKDEEKAKYIFSLFSSESGSYVVREEMERMLHVVDGKVPDTLRKCFSEGEKVNYEKFRNWLLLNKDAFTFSRWLLSGGVYVTLTDDSDTPTFYQTLAGVTHLEESDIIDLEKRYWLLKAQSRTGRFDLETFGPLVSPPIRPSLSEGLFNAFDENRDNHIDFKEISCGLSACCRGPLAERQKFCFKVFDVDRDGVLSRVELKDMVVALLEVWKDNRTDDIPELHMDLSDIVERILNAHDTTKVGHLTLEDYQIWSVKNVLANEFLNLLFQVCHIVLGLRPATPEEEGQIIRGWLERESRYGLQPGHNWFIISMQWWQQWKEYVKYDASPVVIEPSSVLNGGKFSFGTAAHPIEHGEDRISNNLGYMNTTEEKYSDNISSASEASESTGSGFLYSGTPGADMCFARQHNTSDNNNQCLLGANGNILLHLNPQKPGAIDNQPLVTQEPVKATSLTLEGGRLKRTPQLIHGRDYEMVPEPVWRALYHWYGSNLALPRPVIKNSKTDIPELELFPRYLLFLRQQPATRTQQSNIWVNMGMMSLRMFPQHLPRGNVPSPNAPLKRVLAYTGCFSRMQTIKEIHEYLSQRLRIKEEDMRLWLYNSENYLTLLDDEDHRLEYLKIQDEQHLVIEVRNKDMSWPEEMSFIANSSKIDRHKVPTEKGATGLSNLGNTCFMNSSIQCVSNTQPLTQYFISGRHLYELNRTNPIGMKGHMAKCYGDLVQELWSGTQKNVAPLKLRWTIAKYAPRFNGFQQQDSQELLAFLLDGLHEDLNRVHEKPYVELKDSDGRPDWEVAAEAWDNHLRRNRSIVVPQIYVAQFKFEQKCSVVRV

Target ORF information:

RefSeq Version	XM_011248991.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin specific peptidase 32 (Usp32), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248991.1

ORF Insert Sequence:

ATGGGCGCCA AGGAGTCACG GATCGGATTC CTCAGCTACG AGGAGGCGCT GAGGAGAGTT 
ACAGATGTTG AGCTGAAACG ACTCAAAGAT GCCTTCAAGA GGACCTGCGG CCTCTCCTAT 
TACATGAGTC AGCACTGCTT CATCAGGGAA GTGCTTGGGG ATGGAGTACC TCCTAAAGTT 
GCTGAGGTGA TTTACTGTTC TTTTGGTGGA ACATCCAAAG GGCTGCATTT CAATAACTTA 
ATAGTTGGAC TCGTCCTCCT TACAAGAGGC AAAGATGAAG AGAAAGCAAA ATATATTTTT 
AGTCTTTTTT CAAGTGAATC CGGGAGCTAT GTTGTACGGG AAGAAATGGA GAGAATGCTC 
CATGTGGTGG ATGGTAAAGT CCCAGATACA CTCAGGAAGT GTTTCTCAGA GGGTGAAAAG 
GTAAACTATG AAAAATTCAG AAATTGGCTT CTTCTAAACA AAGATGCTTT TACCTTCTCT 
CGCTGGCTGT TGTCTGGGGG TGTATATGTG ACCCTCACTG ATGACAGTGA CACTCCTACT 
TTCTACCAAA CTCTGGCTGG AGTCACACAT TTGGAAGAGT CAGACATCAT CGATCTGGAG 
AAACGGTATT GGCTACTGAA GGCCCAATCC CGAACTGGAC GTTTTGACTT AGAGACCTTT 
GGTCCATTGG TTTCACCACC TATTCGCCCT TCTCTGAGTG AAGGATTATT TAATGCTTTT 
GATGAAAATC GTGACAATCA CATAGACTTC AAGGAGATAT CCTGTGGGTT ATCAGCCTGC 
TGCAGGGGCC CCCTGGCTGA AAGACAAAAA TTTTGCTTCA AGGTGTTTGA TGTTGACCGT 
GATGGAGTTC TCTCCAGGGT TGAACTGAAA GACATGGTAG TTGCACTTTT GGAGGTCTGG 
AAAGATAACC GCACTGATGA TATTCCTGAA TTGCATATGG ATTTGTCAGA CATTGTAGAA 
AGGATATTGA ATGCACATGA TACCACAAAG GTGGGCCATC TTACTCTTGA AGACTATCAG 
ATATGGAGTG TGAAAAATGT TCTTGCCAAT GAGTTTTTGA ATCTCCTCTT CCAGGTATGC 
CACATAGTTC TGGGGTTAAG ACCAGCTACT CCAGAAGAAG AAGGACAAAT TATTAGAGGA 
TGGTTGGAAC GAGAAAGCAG GTATGGTCTG CAGCCAGGAC ACAATTGGTT TATCATCTCC 
ATGCAGTGGT GGCAACAGTG GAAAGAATAT GTCAAATATG ATGCCAGCCC TGTTGTAATT 
GAGCCATCAT CTGTTCTAAA TGGAGGAAAA TTTTCATTTG GAACAGCAGC ACATCCTATA 
GAGCATGGAG AAGATAGAAT CAGTAATAAC CTTGGTTATA TGAATACCAC AGAAGAGAAA 
TACTCAGACA ACATTTCTTC TGCATCTGAA GCCTCAGAGT CTACTGGCAG TGGCTTTCTG 
TATTCTGGCA CACCAGGGGC AGATATGTGC TTTGCAAGGC AGCATAACAC TTCTGACAAT 
AACAACCAGT GTTTACTAGG AGCCAATGGG AATATCCTTT TGCACCTTAA TCCTCAGAAG 
CCAGGAGCAA TTGATAACCA GCCACTAGTA ACTCAGGAAC CAGTAAAGGC TACATCATTA 
ACACTAGAAG GAGGGCGATT AAAACGAACT CCACAGCTAA TCCATGGAAG AGATTATGAA 
ATGGTCCCAG AACCCGTGTG GAGAGCACTT TATCATTGGT ATGGATCAAA CCTGGCCCTT 
CCTAGACCAG TAATCAAGAA TAGCAAAACG GACATCCCAG AACTAGAATT ATTTCCTCGC 
TATCTTCTCT TCCTTCGACA GCAGCCTGCC ACACGGACAC AGCAATCTAA CATCTGGGTG 
AATATGGGTA TGATGAGCCT GAGAATGTTT CCTCAGCATT TGCCTAGAGG AAATGTACCC 
TCTCCAAATG CGCCTCTAAA GCGAGTGTTA GCCTATACAG GCTGTTTTAG TAGAATGCAA 
ACCATCAAGG AAATTCATGA ATATCTGTCT CAAAGGCTTC GCATTAAAGA GGAAGATATG 
CGGCTGTGGC TGTACAATAG TGAGAACTAC CTGACTCTTC TGGATGATGA GGATCACAGA 
TTGGAATATT TGAAAATCCA GGATGAGCAG CACCTGGTAA TTGAAGTTCG AAACAAAGAT 
ATGAGTTGGC CTGAAGAAAT GTCTTTTATA GCAAACAGTA GCAAAATAGA TAGACATAAA 
GTTCCCACAG AAAAGGGCGC CACAGGTCTG AGTAACCTGG GAAACACATG CTTCATGAAC 
TCAAGCATCC AGTGTGTCAG CAATACACAA CCACTGACAC AGTACTTTAT CTCAGGGAGG 
CATCTTTATG AACTCAACAG GACAAATCCC ATTGGTATGA AAGGCCATAT GGCTAAATGT 
TATGGCGATT TGGTGCAAGA ACTTTGGAGT GGAACTCAGA AGAATGTTGC CCCCTTAAAG 
CTCCGGTGGA CCATAGCAAA ATATGCTCCC AGGTTTAATG GATTCCAGCA ACAAGACTCC 
CAAGAACTTC TGGCTTTCCT CTTGGATGGT CTTCATGAAG ATCTCAACCG AGTCCACGAG 
AAACCATATG TAGAACTAAA AGACAGTGAC GGCCGACCCG ACTGGGAAGT AGCTGCAGAG 
GCCTGGGACA ACCATCTAAG GAGAAATAGA TCAATTGTTG TACCACAAAT ATATGTGGCA 
CAATTTAAAT TTGAACAAAA GTGTTCTGTG GTTAGAGTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Usp32 ( XM_030245969.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030101829.1 Mus musculus ubiquitin specific peptidase 32 (Usp32), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu85780
Clone ID Related Accession (Same CDS sequence)	XM_030245969.1
Accession Version	XM_030245969.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4734bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ubiquitin carboxyl-terminal hydrolase 32 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCAGC ACTGCTTCAT CAGGGAAGTG CTTGGGGATG GAGTACCTCC TAAAGTTGCT 
GAGGTGATTT ACTGTTCTTT TGGTGGAACA TCCAAAGGGC TGCATTTCAA TAACTTAATA 
GTTGGACTCG TCCTCCTTAC AAGAGGCAAA GATGAAGAGA AAGCAAAATA TATTTTTAGT 
CTTTTTTCAA GTGAATCCGG GAGCTATGTT GTACGGGAAG AAATGGAGAG AATGCTCCAT 
GTGGTGGATG GTAAAGTCCC AGATACACTC AGGAAGTGTT TCTCAGAGGG TGAAAAGGTA 
AACTATGAAA AATTCAGAAA TTGGCTTCTT CTAAACAAAG ATGCTTTTAC CTTCTCTCGC 
TGGCTGTTGT CTGGGGGTGT ATATGTGACC CTCACTGATG ACAGTGACAC TCCTACTTTC 
TACCAAACTC TGGCTGGAGT CACACATTTG GAAGAGTCAG ACATCATCGA TCTGGAGAAA 
CGGTATTGGC TACTGAAGGC CCAATCCCGA ACTGGACGTT TTGACTTAGA GACCTTTGGT 
CCATTGGTTT CACCACCTAT TCGCCCTTCT CTGAGTGAAG GATTATTTAA TGCTTTTGAT 
GAAAATCGTG ACAATCACAT AGACTTCAAG GAGATATCCT GTGGGTTATC AGCCTGCTGC 
AGGGGCCCCC TGGCTGAAAG ACAAAAATTT TGCTTCAAGG TGTTTGATGT TGACCGTGAT 
GGAGTTCTCT CCAGGGTTGA ACTGAAAGAC ATGGTAGTTG CACTTTTGGA GGTCTGGAAA 
GATAACCGCA CTGATGATAT TCCTGAATTG CATATGGATT TGTCAGACAT TGTAGAAAGG 
ATATTGAATG CACATGATAC CACAAAGGTG GGCCATCTTA CTCTTGAAGA CTATCAGATA 
TGGAGTGTGA AAAATGTTCT TGCCAATGAG TTTTTGAATC TCCTCTTCCA GGTATGCCAC 
ATAGTTCTGG GGTTAAGACC AGCTACTCCA GAAGAAGAAG GACAAATTAT TAGAGGATGG 
TTGGAACGAG AAAGCAGGTA TGGTCTGCAG CCAGGACACA ATTGGTTTAT CATCTCCATG 
CAGTGGTGGC AACAGTGGAA AGAATATGTC AAATATGATG CCAGCCCTGT TGTAATTGAG 
CCATCATCTG TTCTAAATGG AGGAAAATTT TCATTTGGAA CAGCAGCACA TCCTATAGAG 
CATGGAGAAG ATAGAATCAG TAATAACCTT GGTTATATGA ATACCACAGA AGAGAAATAC 
TCAGACAACA TTTCTTCTGC ATCTGAAGCC TCAGAGTCTA CTGGCAGTGG CTTTCTGTAT 
TCTGGCACAC CAGGGGCAGA TATGTGCTTT GCAAGGCAGC ATAACACTTC TGACAATAAC 
AACCAGTGTT TACTAGGAGC CAATGGGAAT ATCCTTTTGC ACCTTAATCC TCAGAAGCCA 
GGAGCAATTG ATAACCAGCC ACTAGTAACT CAGGAACCAG TAAAGGCTAC ATCATTAACA 
CTAGAAGGAG GGCGATTAAA ACGAACTCCA CAGCTAATCC ATGGAAGAGA TTATGAAATG 
GTCCCAGAAC CCGTGTGGAG AGCACTTTAT CATTGGTATG GATCAAACCT GGCCCTTCCT 
AGACCAGTAA TCAAGAATAG CAAAACGGAC ATCCCAGAAC TAGAATTATT TCCTCGCTAT 
CTTCTCTTCC TTCGACAGCA GCCTGCCACA CGGACACAGC AATCTAACAT CTGGGTGAAT 
ATGGGTATGA TGAGCCTGAG AATGTTTCCT CAGCATTTGC CTAGAGGAAA TGTACCCTCT 
CCAAATGCGC CTCTAAAGCG AGTGTTAGCC TATACAGGCT GTTTTAGTAG AATGCAAACC 
ATCAAGGAAA TTCATGAATA TCTGTCTCAA AGGCTTCGCA TTAAAGAGGA AGATATGCGG 
CTGTGGCTGT ACAATAGTGA GAACTACCTG ACTCTTCTGG ATGATGAGGA TCACAGATTG 
GAATATTTGA AAATCCAGGA TGAGCAGCAC CTGGTAATTG AAGTTCGAAA CAAAGATATG 
AGTTGGCCTG AAGAAATGTC TTTTATAGCA AACAGTAGCA AAATAGATAG ACATAAAGTT 
CCCACAGAAA AGGGCGCCAC AGGTCTGAGT AACCTGGGAA ACACATGCTT CATGAACTCA 
AGCATCCAGT GTGTCAGCAA TACACAACCA CTGACACAGT ACTTTATCTC AGGGAGGCAT 
CTTTATGAAC TCAACAGGAC AAATCCCATT GGTATGAAAG GCCATATGGC TAAATGTTAT 
GGCGATTTGG TGCAAGAACT TTGGAGTGGA ACTCAGAAGA ATGTTGCCCC CTTAAAGCTC 
CGGTGGACCA TAGCAAAATA TGCTCCCAGG TTTAATGGAT TCCAGCAACA AGACTCCCAA 
GAACTTCTGG CTTTCCTCTT GGATGGTCTT CATGAAGATC TCAACCGAGT CCACGAGAAA 
CCATATGTAG AACTAAAAGA CAGTGACGGC CGACCCGACT GGGAAGTAGC TGCAGAGGCC 
TGGGACAACC ATCTAAGGAG AAATAGATCA ATTGTTGTGG ACTTGTTCCA TGGTCAGCTA 
AGATCCCAAG TCAAATGCAA GACATGTGGG CATATAAGTG TCCGATTCGA CCCTTTTAAT 
TTTTTGTCTT TACCACTACC AATGGACAGT TACATGCATT TAGAAATAAC AGTAATTAAA 
TTAGATGGTA CTACCCCCAT ACGATATGGA CTAAGACTGA ATATGGATGA AAAGTATACA 
GGTTTGAAAA AGCAGCTGAG TGATCTCTGT GGACTTAAAT CAGAACAAAT CCTTTTTGCA 
GAAGTGCATA GTTCAAACAT AAAGAACTTC CCTCAGGACA ACCAAAAAGT TCGACTCTCA 
GTGAGTGGAT TTTTGTGTGC ATTTGAAATT CCTATTCCTG CATCTCCAGT TTCAGCTTGC 
AGTCCAATTC AAACAGATTG CTCTTCTTCT CCATCTACAA ATGGACTGTT TACCTTGACT 
ACCAATGGGG ATCTGCCTCG ACCTATATTC ATCCCCAATG GAATGCCAAA TACAGTTGTA 
CCATGTGGAA CTGAGAAGAA TGTCACAAAT GGAATAGTGA ATGGTCATAT GCCGCCTCTT 
CCTGATGACC CCTTTACAGG CTACATCATT GCAGTCCACC GAAAAATGAT GAGGACAGAA 
CTGTATTTCC TGTCATCTCA GAAGAATCGC CCCAGTCTCT TTGGGATGCC ACTGATTGTT 
CCATGTACTG TACATACCCG GAAAAAAGAT CTCTATGATG CAGTTTGGAT TCAAGTATCT 
CGATTAGCCA GTCCGCTCCC ACCTCAAGAA GCTAGCAACC ATGCACAGGA TTGTGATGAC 
AGCATGGGCT ATCAATATCC ATTCACTCTC CGAGTTGTGC AGAAAGATGG AAACTCCTGT 
GCCTGGTGTC CATGGTATAG ATTTTGCAGA GGCTGTAAAA TCGATTGTGG AGAAGACAGA 
GCTTTCATTG GAAATGCCTG TATTGCTGTG GATTGGGACC CTACAGCCCT TCACCTCCGC 
TATCAAACAT CACAGGAAAG AGTTGTAGAG GAGCATGAAA GTGTGGAACA GAGTCGGCGA 
GCACAAGCTG AGCCCATCAA CCTGGACAGC TGTCTCCGTG CCTTCACCAG TGAGGAGGAA 
CTGGGGGAAA ATGAGATGTA CTACTGCTCC AAATGTAAAA CACACTGTTT GGCAACCAAG 
AAACTGGACC TCTGGAGGCT TCCTCCAATC CTGATTATTC ACCTTAAACG ATTTCAGTTT 
GTAAATGGAC GGTGGATAAA ATCACAGAAA ATTGTTAAAT TTCCTCGTGA AAGTTTTGAC 
CCTAGTGCTT TTTTAGTACC AAGAGACCCA ACTCTTTGCC AGCATAAACC ACTCACACCC 
CAGGGGGATG ACTTCTCTGA GCTAAGGATC CCAGCAGGAG ATGTGAAGAA AGTGGATATC 
CAGAGCTCAG CAGGGGAGGA GGATGTGCTC CTGAGCAAGA GCCCATCTTC ACTCAGTGCA 
AATGTCACCA GTAGCCCAAA AGGTTCACCT TCTTCATCAA GAAAAAGTGG AGCCAGCTGT 
CCCTCCAGCA AAAATAGCAG CCCTAACAGC AGCCCACGGA CCTTGGGGAG GAACAAAGGG 
AGGCTCCGGC TGCCTCAAAT TGGCAGCAAA AATAAATTGT CCAATAGTAA GGAGAATTTG 
GATACCAGCA AAGAGAATGG GGCTGGGCAG ATCTGTGAAC TGGCTGACAC CTTGAACCGA 
AGACATGTAT TGGGAGGCAG CCAGCCTGAG CTGGTCACTC CTCTGGATCA TGAGATCACT 
TTGGCTAATG GATTTCTTTA TGAGCATGAG GCATGTGGCA ATGGCTATAG CAATGGTCAG 
CTTGGAAACC ATAGTGAAGA AGACAGCACT GATGACCAAA GAGAAGAAAC ACACAGTAAA 
CCTATTTATA ATCTATATGC AATTTCGTGC CATTCAGGAA TTCTGGGTGG GGGCCATTAC 
GTCACTTATG CCAAAAACCC AAACTGCAAG TGGTACTGTT ACAATGACAG CAGCTGTAAG 
GAGCTTCACC CTGATGAGAT AGACACCGAC TCTGCCTACA TTCTTTTCTA TGAGCAGCAG 
GGGATAGACT GTGCACAGTT TCTGCCAAAG ACAGACGGCA AGAAGATGGC GGACACGAGC 
AGCATGGACG AAGACTTTGA GTCTGACTAC AAGAAGTACT GTGTGCTACA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030101829.1
CDS	245..4978
Translation	MSQHCFIREVLGDGVPPKVAEVIYCSFGGTSKGLHFNNLIVGLVLLTRGKDEEKAKYIFSLFSSESGSYVVREEMERMLHVVDGKVPDTLRKCFSEGEKVNYEKFRNWLLLNKDAFTFSRWLLSGGVYVTLTDDSDTPTFYQTLAGVTHLEESDIIDLEKRYWLLKAQSRTGRFDLETFGPLVSPPIRPSLSEGLFNAFDENRDNHIDFKEISCGLSACCRGPLAERQKFCFKVFDVDRDGVLSRVELKDMVVALLEVWKDNRTDDIPELHMDLSDIVERILNAHDTTKVGHLTLEDYQIWSVKNVLANEFLNLLFQVCHIVLGLRPATPEEEGQIIRGWLERESRYGLQPGHNWFIISMQWWQQWKEYVKYDASPVVIEPSSVLNGGKFSFGTAAHPIEHGEDRISNNLGYMNTTEEKYSDNISSASEASESTGSGFLYSGTPGADMCFARQHNTSDNNNQCLLGANGNILLHLNPQKPGAIDNQPLVTQEPVKATSLTLEGGRLKRTPQLIHGRDYEMVPEPVWRALYHWYGSNLALPRPVIKNSKTDIPELELFPRYLLFLRQQPATRTQQSNIWVNMGMMSLRMFPQHLPRGNVPSPNAPLKRVLAYTGCFSRMQTIKEIHEYLSQRLRIKEEDMRLWLYNSENYLTLLDDEDHRLEYLKIQDEQHLVIEVRNKDMSWPEEMSFIANSSKIDRHKVPTEKGATGLSNLGNTCFMNSSIQCVSNTQPLTQYFISGRHLYELNRTNPIGMKGHMAKCYGDLVQELWSGTQKNVAPLKLRWTIAKYAPRFNGFQQQDSQELLAFLLDGLHEDLNRVHEKPYVELKDSDGRPDWEVAAEAWDNHLRRNRSIVVDLFHGQLRSQVKCKTCGHISVRFDPFNFLSLPLPMDSYMHLEITVIKLDGTTPIRYGLRLNMDEKYTGLKKQLSDLCGLKSEQILFAEVHSSNIKNFPQDNQKVRLSVSGFLCAFEIPIPASPVSACSPIQTDCSSSPSTNGLFTLTTNGDLPRPIFIPNGMPNTVVPCGTEKNVTNGIVNGHMPPLPDDPFTGYIIAVHRKMMRTELYFLSSQKNRPSLFGMPLIVPCTVHTRKKDLYDAVWIQVSRLASPLPPQEASNHAQDCDDSMGYQYPFTLRVVQKDGNSCAWCPWYRFCRGCKIDCGEDRAFIGNACIAVDWDPTALHLRYQTSQERVVEEHESVEQSRRAQAEPINLDSCLRAFTSEEELGENEMYYCSKCKTHCLATKKLDLWRLPPILIIHLKRFQFVNGRWIKSQKIVKFPRESFDPSAFLVPRDPTLCQHKPLTPQGDDFSELRIPAGDVKKVDIQSSAGEEDVLLSKSPSSLSANVTSSPKGSPSSSRKSGASCPSSKNSSPNSSPRTLGRNKGRLRLPQIGSKNKLSNSKENLDTSKENGAGQICELADTLNRRHVLGGSQPELVTPLDHEITLANGFLYEHEACGNGYSNGQLGNHSEEDSTDDQREETHSKPIYNLYAISCHSGILGGGHYVTYAKNPNCKWYCYNDSSCKELHPDEIDTDSAYILFYEQQGIDCAQFLPKTDGKKMADTSSMDEDFESDYKKYCVLQ

Target ORF information:

RefSeq Version	XM_030245969.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin specific peptidase 32 (Usp32), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030245969.1

ORF Insert Sequence:

ATGAGTCAGC ACTGCTTCAT CAGGGAAGTG CTTGGGGATG GAGTACCTCC TAAAGTTGCT 
GAGGTGATTT ACTGTTCTTT TGGTGGAACA TCCAAAGGGC TGCATTTCAA TAACTTAATA 
GTTGGACTCG TCCTCCTTAC AAGAGGCAAA GATGAAGAGA AAGCAAAATA TATTTTTAGT 
CTTTTTTCAA GTGAATCCGG GAGCTATGTT GTACGGGAAG AAATGGAGAG AATGCTCCAT 
GTGGTGGATG GTAAAGTCCC AGATACACTC AGGAAGTGTT TCTCAGAGGG TGAAAAGGTA 
AACTATGAAA AATTCAGAAA TTGGCTTCTT CTAAACAAAG ATGCTTTTAC CTTCTCTCGC 
TGGCTGTTGT CTGGGGGTGT ATATGTGACC CTCACTGATG ACAGTGACAC TCCTACTTTC 
TACCAAACTC TGGCTGGAGT CACACATTTG GAAGAGTCAG ACATCATCGA TCTGGAGAAA 
CGGTATTGGC TACTGAAGGC CCAATCCCGA ACTGGACGTT TTGACTTAGA GACCTTTGGT 
CCATTGGTTT CACCACCTAT TCGCCCTTCT CTGAGTGAAG GATTATTTAA TGCTTTTGAT 
GAAAATCGTG ACAATCACAT AGACTTCAAG GAGATATCCT GTGGGTTATC AGCCTGCTGC 
AGGGGCCCCC TGGCTGAAAG ACAAAAATTT TGCTTCAAGG TGTTTGATGT TGACCGTGAT 
GGAGTTCTCT CCAGGGTTGA ACTGAAAGAC ATGGTAGTTG CACTTTTGGA GGTCTGGAAA 
GATAACCGCA CTGATGATAT TCCTGAATTG CATATGGATT TGTCAGACAT TGTAGAAAGG 
ATATTGAATG CACATGATAC CACAAAGGTG GGCCATCTTA CTCTTGAAGA CTATCAGATA 
TGGAGTGTGA AAAATGTTCT TGCCAATGAG TTTTTGAATC TCCTCTTCCA GGTATGCCAC 
ATAGTTCTGG GGTTAAGACC AGCTACTCCA GAAGAAGAAG GACAAATTAT TAGAGGATGG 
TTGGAACGAG AAAGCAGGTA TGGTCTGCAG CCAGGACACA ATTGGTTTAT CATCTCCATG 
CAGTGGTGGC AACAGTGGAA AGAATATGTC AAATATGATG CCAGCCCTGT TGTAATTGAG 
CCATCATCTG TTCTAAATGG AGGAAAATTT TCATTTGGAA CAGCAGCACA TCCTATAGAG 
CATGGAGAAG ATAGAATCAG TAATAACCTT GGTTATATGA ATACCACAGA AGAGAAATAC 
TCAGACAACA TTTCTTCTGC ATCTGAAGCC TCAGAGTCTA CTGGCAGTGG CTTTCTGTAT 
TCTGGCACAC CAGGGGCAGA TATGTGCTTT GCAAGGCAGC ATAACACTTC TGACAATAAC 
AACCAGTGTT TACTAGGAGC CAATGGGAAT ATCCTTTTGC ACCTTAATCC TCAGAAGCCA 
GGAGCAATTG ATAACCAGCC ACTAGTAACT CAGGAACCAG TAAAGGCTAC ATCATTAACA 
CTAGAAGGAG GGCGATTAAA ACGAACTCCA CAGCTAATCC ATGGAAGAGA TTATGAAATG 
GTCCCAGAAC CCGTGTGGAG AGCACTTTAT CATTGGTATG GATCAAACCT GGCCCTTCCT 
AGACCAGTAA TCAAGAATAG CAAAACGGAC ATCCCAGAAC TAGAATTATT TCCTCGCTAT 
CTTCTCTTCC TTCGACAGCA GCCTGCCACA CGGACACAGC AATCTAACAT CTGGGTGAAT 
ATGGGTATGA TGAGCCTGAG AATGTTTCCT CAGCATTTGC CTAGAGGAAA TGTACCCTCT 
CCAAATGCGC CTCTAAAGCG AGTGTTAGCC TATACAGGCT GTTTTAGTAG AATGCAAACC 
ATCAAGGAAA TTCATGAATA TCTGTCTCAA AGGCTTCGCA TTAAAGAGGA AGATATGCGG 
CTGTGGCTGT ACAATAGTGA GAACTACCTG ACTCTTCTGG ATGATGAGGA TCACAGATTG 
GAATATTTGA AAATCCAGGA TGAGCAGCAC CTGGTAATTG AAGTTCGAAA CAAAGATATG 
AGTTGGCCTG AAGAAATGTC TTTTATAGCA AACAGTAGCA AAATAGATAG ACATAAAGTT 
CCCACAGAAA AGGGCGCCAC AGGTCTGAGT AACCTGGGAA ACACATGCTT CATGAACTCA 
AGCATCCAGT GTGTCAGCAA TACACAACCA CTGACACAGT ACTTTATCTC AGGGAGGCAT 
CTTTATGAAC TCAACAGGAC AAATCCCATT GGTATGAAAG GCCATATGGC TAAATGTTAT 
GGCGATTTGG TGCAAGAACT TTGGAGTGGA ACTCAGAAGA ATGTTGCCCC CTTAAAGCTC 
CGGTGGACCA TAGCAAAATA TGCTCCCAGG TTTAATGGAT TCCAGCAACA AGACTCCCAA 
GAACTTCTGG CTTTCCTCTT GGATGGTCTT CATGAAGATC TCAACCGAGT CCACGAGAAA 
CCATATGTAG AACTAAAAGA CAGTGACGGC CGACCCGACT GGGAAGTAGC TGCAGAGGCC 
TGGGACAACC ATCTAAGGAG AAATAGATCA ATTGTTGTGG ACTTGTTCCA TGGTCAGCTA 
AGATCCCAAG TCAAATGCAA GACATGTGGG CATATAAGTG TCCGATTCGA CCCTTTTAAT 
TTTTTGTCTT TACCACTACC AATGGACAGT TACATGCATT TAGAAATAAC AGTAATTAAA 
TTAGATGGTA CTACCCCCAT ACGATATGGA CTAAGACTGA ATATGGATGA AAAGTATACA 
GGTTTGAAAA AGCAGCTGAG TGATCTCTGT GGACTTAAAT CAGAACAAAT CCTTTTTGCA 
GAAGTGCATA GTTCAAACAT AAAGAACTTC CCTCAGGACA ACCAAAAAGT TCGACTCTCA 
GTGAGTGGAT TTTTGTGTGC ATTTGAAATT CCTATTCCTG CATCTCCAGT TTCAGCTTGC 
AGTCCAATTC AAACAGATTG CTCTTCTTCT CCATCTACAA ATGGACTGTT TACCTTGACT 
ACCAATGGGG ATCTGCCTCG ACCTATATTC ATCCCCAATG GAATGCCAAA TACAGTTGTA 
CCATGTGGAA CTGAGAAGAA TGTCACAAAT GGAATAGTGA ATGGTCATAT GCCGCCTCTT 
CCTGATGACC CCTTTACAGG CTACATCATT GCAGTCCACC GAAAAATGAT GAGGACAGAA 
CTGTATTTCC TGTCATCTCA GAAGAATCGC CCCAGTCTCT TTGGGATGCC ACTGATTGTT 
CCATGTACTG TACATACCCG GAAAAAAGAT CTCTATGATG CAGTTTGGAT TCAAGTATCT 
CGATTAGCCA GTCCGCTCCC ACCTCAAGAA GCTAGCAACC ATGCACAGGA TTGTGATGAC 
AGCATGGGCT ATCAATATCC ATTCACTCTC CGAGTTGTGC AGAAAGATGG AAACTCCTGT 
GCCTGGTGTC CATGGTATAG ATTTTGCAGA GGCTGTAAAA TCGATTGTGG AGAAGACAGA 
GCTTTCATTG GAAATGCCTG TATTGCTGTG GATTGGGACC CTACAGCCCT TCACCTCCGC 
TATCAAACAT CACAGGAAAG AGTTGTAGAG GAGCATGAAA GTGTGGAACA GAGTCGGCGA 
GCACAAGCTG AGCCCATCAA CCTGGACAGC TGTCTCCGTG CCTTCACCAG TGAGGAGGAA 
CTGGGGGAAA ATGAGATGTA CTACTGCTCC AAATGTAAAA CACACTGTTT GGCAACCAAG 
AAACTGGACC TCTGGAGGCT TCCTCCAATC CTGATTATTC ACCTTAAACG ATTTCAGTTT 
GTAAATGGAC GGTGGATAAA ATCACAGAAA ATTGTTAAAT TTCCTCGTGA AAGTTTTGAC 
CCTAGTGCTT TTTTAGTACC AAGAGACCCA ACTCTTTGCC AGCATAAACC ACTCACACCC 
CAGGGGGATG ACTTCTCTGA GCTAAGGATC CCAGCAGGAG ATGTGAAGAA AGTGGATATC 
CAGAGCTCAG CAGGGGAGGA GGATGTGCTC CTGAGCAAGA GCCCATCTTC ACTCAGTGCA 
AATGTCACCA GTAGCCCAAA AGGTTCACCT TCTTCATCAA GAAAAAGTGG AGCCAGCTGT 
CCCTCCAGCA AAAATAGCAG CCCTAACAGC AGCCCACGGA CCTTGGGGAG GAACAAAGGG 
AGGCTCCGGC TGCCTCAAAT TGGCAGCAAA AATAAATTGT CCAATAGTAA GGAGAATTTG 
GATACCAGCA AAGAGAATGG GGCTGGGCAG ATCTGTGAAC TGGCTGACAC CTTGAACCGA 
AGACATGTAT TGGGAGGCAG CCAGCCTGAG CTGGTCACTC CTCTGGATCA TGAGATCACT 
TTGGCTAATG GATTTCTTTA TGAGCATGAG GCATGTGGCA ATGGCTATAG CAATGGTCAG 
CTTGGAAACC ATAGTGAAGA AGACAGCACT GATGACCAAA GAGAAGAAAC ACACAGTAAA 
CCTATTTATA ATCTATATGC AATTTCGTGC CATTCAGGAA TTCTGGGTGG GGGCCATTAC 
GTCACTTATG CCAAAAACCC AAACTGCAAG TGGTACTGTT ACAATGACAG CAGCTGTAAG 
GAGCTTCACC CTGATGAGAT AGACACCGAC TCTGCCTACA TTCTTTTCTA TGAGCAGCAG 
GGGATAGACT GTGCACAGTT TCTGCCAAAG ACAGACGGCA AGAAGATGGC GGACACGAGC 
AGCATGGACG AAGACTTTGA GTCTGACTAC AAGAAGTACT GTGTGCTACA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Usp32 ( XM_030245970.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030101830.1 Mus musculus ubiquitin specific peptidase 32 (Usp32), transcript variant X3, mRNA.

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CloneID	OMu85781
Clone ID Related Accession (Same CDS sequence)	XM_030245970.1
Accession Version	XM_030245970.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4692bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ubiquitin carboxyl-terminal hydrolase 32 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCAGC ACTGCTTCAT CAGGGAAGTG CTTGGGGATG GAGTACCTCC TAAAGTTGCT 
GAGGTGATTT ACTGTTCTTT TGGTGGAACA TCCAAAGGGC TGCATTTCAA TAACTTAATA 
GTTGGACTCG TCCTCCTTAC AAGAGGCAAA GATGAAGAGA AAGCAAAATA TATTTTTAGT 
CTTTTTTCAA GTGAATCCGG GAGCTATGTT GTACGGGAAG AAATGGAGAG AATGCTCCAT 
GTGGTGGATG GTAAAGTCCC AGATACACTC AGGAAGTGTT TCTCAGAGGG TGAAAAGGTA 
AACTATGAAA AATTCAGAAA TTGGCTTCTT CTAAACAAAG ATGCTTTTAC CTTCTCTCGC 
TGGCTGTTGT CTGGGGGTGT ATATGTGACC CTCACTGATG ACAGTGACAC TCCTACTTTC 
TACCAAACTC TGGCTGGAGT CACACATTTG GAAGAGTCAG ACATCATCGA TCTGGAGAAA 
CGGTATTGGC TACTGAAGGC CCAATCCCGA ACTGGACGTT TTGACTTAGA GACCTTTGGT 
CCATTGGTTT CACCACCTAT TCGCCCTTCT CTGAGTGAAG GATTATTTAA TGCTTTTGAT 
GAAAATCGTG ACAATCACAT AGACTTCAAG GAGATATCCT GTGGGTTATC AGCCTGCTGC 
AGGGGCCCCC TGGCTGAAAG ACAAAAATTT TGCTTCAAGG TGTTTGATGT TGACCGTGAT 
GGAGTTCTCT CCAGGGTTGA ACTGAAAGAC ATGGTAGTTG CACTTTTGGA GGTCTGGAAA 
GATAACCGCA CTGATGATAT TCCTGAATTG CATATGGATT TGTCAGACAT TGTAGAAAGG 
ATATTGAATG CACATGATAC CACAAAGGTG GGCCATCTTA CTCTTGAAGA CTATCAGATA 
TGGAGTGTGA AAAATGTTCT TGCCAATGAG TTTTTGAATC TCCTCTTCCA GGTATGCCAC 
ATAGTTCTGG GGTTAAGACC AGCTACTCCA GAAGAAGAAG GACAAATTAT TAGAGGATGG 
TTGGAACGAG AAAGCAGGTA TGGTCTGCAG CCAGGACACA ATTGGTTTAT CATCTCCATG 
CAGTGGTGGC AACAGTGGAA AGAATATGTC AAATATGATG CCAGCCCTGT TGTAATTGAG 
CCATCATCTG TTCTAAATGG AGGAAAATTT TCATTTGGAA CAGCAGCACA TCCTATAGAG 
CATGGAGAAG ATAGAATCAG TAATAACCTT GGTTATATGA ATACCACAGA AGAGAAATAC 
TCAGACAACA TTTCTTCTGC ATCTGAAGCC TCAGAGTCTA CTGGCAGTGG CTTTCTGTAT 
TCTGGCACAC CAGGGGCAGA TATGTGCTTT GCAAGGCAGC ATAACACTTC TGACAATAAC 
AACCAGTGTT TACTAGGAGC CAATGGGAAT ATCCTTTTGC ACCTTAATCC TCAGAAGCCA 
GGAGCAATTG ATAACCAGCC ACTAGTAACT CAGGAACCAG TAAAGGCTAC ATCATTAACA 
CTAGAAGGAG GGCGATTAAA ACGAACTCCA CAGCTAATCC ATGGAAGAGA TTATGAAATG 
GTCCCAGAAC CCGTGTGGAG AGCACTTTAT CATTGGTATG GATCAAACCT GGCCCTTCCT 
AGACCAGTAA TCAAGAATAG CAAAACGGAC ATCCCAGAAC TAGAATTATT TCCTCGCTAT 
CTTCTCTTCC TTCGACAGCA GCCTGCCACA CGGACACAGC AATCTAACAT CTGGGTGAAT 
ATGGGAAATG TACCCTCTCC AAATGCGCCT CTAAAGCGAG TGTTAGCCTA TACAGGCTGT 
TTTAGTAGAA TGCAAACCAT CAAGGAAATT CATGAATATC TGTCTCAAAG GCTTCGCATT 
AAAGAGGAAG ATATGCGGCT GTGGCTGTAC AATAGTGAGA ACTACCTGAC TCTTCTGGAT 
GATGAGGATC ACAGATTGGA ATATTTGAAA ATCCAGGATG AGCAGCACCT GGTAATTGAA 
GTTCGAAACA AAGATATGAG TTGGCCTGAA GAAATGTCTT TTATAGCAAA CAGTAGCAAA 
ATAGATAGAC ATAAAGTTCC CACAGAAAAG GGCGCCACAG GTCTGAGTAA CCTGGGAAAC 
ACATGCTTCA TGAACTCAAG CATCCAGTGT GTCAGCAATA CACAACCACT GACACAGTAC 
TTTATCTCAG GGAGGCATCT TTATGAACTC AACAGGACAA ATCCCATTGG TATGAAAGGC 
CATATGGCTA AATGTTATGG CGATTTGGTG CAAGAACTTT GGAGTGGAAC TCAGAAGAAT 
GTTGCCCCCT TAAAGCTCCG GTGGACCATA GCAAAATATG CTCCCAGGTT TAATGGATTC 
CAGCAACAAG ACTCCCAAGA ACTTCTGGCT TTCCTCTTGG ATGGTCTTCA TGAAGATCTC 
AACCGAGTCC ACGAGAAACC ATATGTAGAA CTAAAAGACA GTGACGGCCG ACCCGACTGG 
GAAGTAGCTG CAGAGGCCTG GGACAACCAT CTAAGGAGAA ATAGATCAAT TGTTGTGGAC 
TTGTTCCATG GTCAGCTAAG ATCCCAAGTC AAATGCAAGA CATGTGGGCA TATAAGTGTC 
CGATTCGACC CTTTTAATTT TTTGTCTTTA CCACTACCAA TGGACAGTTA CATGCATTTA 
GAAATAACAG TAATTAAATT AGATGGTACT ACCCCCATAC GATATGGACT AAGACTGAAT 
ATGGATGAAA AGTATACAGG TTTGAAAAAG CAGCTGAGTG ATCTCTGTGG ACTTAAATCA 
GAACAAATCC TTTTTGCAGA AGTGCATAGT TCAAACATAA AGAACTTCCC TCAGGACAAC 
CAAAAAGTTC GACTCTCAGT GAGTGGATTT TTGTGTGCAT TTGAAATTCC TATTCCTGCA 
TCTCCAGTTT CAGCTTGCAG TCCAATTCAA ACAGATTGCT CTTCTTCTCC ATCTACAAAT 
GGACTGTTTA CCTTGACTAC CAATGGGGAT CTGCCTCGAC CTATATTCAT CCCCAATGGA 
ATGCCAAATA CAGTTGTACC ATGTGGAACT GAGAAGAATG TCACAAATGG AATAGTGAAT 
GGTCATATGC CGCCTCTTCC TGATGACCCC TTTACAGGCT ACATCATTGC AGTCCACCGA 
AAAATGATGA GGACAGAACT GTATTTCCTG TCATCTCAGA AGAATCGCCC CAGTCTCTTT 
GGGATGCCAC TGATTGTTCC ATGTACTGTA CATACCCGGA AAAAAGATCT CTATGATGCA 
GTTTGGATTC AAGTATCTCG ATTAGCCAGT CCGCTCCCAC CTCAAGAAGC TAGCAACCAT 
GCACAGGATT GTGATGACAG CATGGGCTAT CAATATCCAT TCACTCTCCG AGTTGTGCAG 
AAAGATGGAA ACTCCTGTGC CTGGTGTCCA TGGTATAGAT TTTGCAGAGG CTGTAAAATC 
GATTGTGGAG AAGACAGAGC TTTCATTGGA AATGCCTGTA TTGCTGTGGA TTGGGACCCT 
ACAGCCCTTC ACCTCCGCTA TCAAACATCA CAGGAAAGAG TTGTAGAGGA GCATGAAAGT 
GTGGAACAGA GTCGGCGAGC ACAAGCTGAG CCCATCAACC TGGACAGCTG TCTCCGTGCC 
TTCACCAGTG AGGAGGAACT GGGGGAAAAT GAGATGTACT ACTGCTCCAA ATGTAAAACA 
CACTGTTTGG CAACCAAGAA ACTGGACCTC TGGAGGCTTC CTCCAATCCT GATTATTCAC 
CTTAAACGAT TTCAGTTTGT AAATGGACGG TGGATAAAAT CACAGAAAAT TGTTAAATTT 
CCTCGTGAAA GTTTTGACCC TAGTGCTTTT TTAGTACCAA GAGACCCAAC TCTTTGCCAG 
CATAAACCAC TCACACCCCA GGGGGATGAC TTCTCTGAGC TAAGGATCCC AGCAGGAGAT 
GTGAAGAAAG TGGATATCCA GAGCTCAGCA GGGGAGGAGG ATGTGCTCCT GAGCAAGAGC 
CCATCTTCAC TCAGTGCAAA TGTCACCAGT AGCCCAAAAG GTTCACCTTC TTCATCAAGA 
AAAAGTGGAG CCAGCTGTCC CTCCAGCAAA AATAGCAGCC CTAACAGCAG CCCACGGACC 
TTGGGGAGGA ACAAAGGGAG GCTCCGGCTG CCTCAAATTG GCAGCAAAAA TAAATTGTCC 
AATAGTAAGG AGAATTTGGA TACCAGCAAA GAGAATGGGG CTGGGCAGAT CTGTGAACTG 
GCTGACACCT TGAACCGAAG ACATGTATTG GGAGGCAGCC AGCCTGAGCT GGTCACTCCT 
CTGGATCATG AGATCACTTT GGCTAATGGA TTTCTTTATG AGCATGAGGC ATGTGGCAAT 
GGCTATAGCA ATGGTCAGCT TGGAAACCAT AGTGAAGAAG ACAGCACTGA TGACCAAAGA 
GAAGAAACAC ACAGTAAACC TATTTATAAT CTATATGCAA TTTCGTGCCA TTCAGGAATT 
CTGGGTGGGG GCCATTACGT CACTTATGCC AAAAACCCAA ACTGCAAGTG GTACTGTTAC 
AATGACAGCA GCTGTAAGGA GCTTCACCCT GATGAGATAG ACACCGACTC TGCCTACATT 
CTTTTCTATG AGCAGCAGGG GATAGACTGT GCACAGTTTC TGCCAAAGAC AGACGGCAAG 
AAGATGGCGG ACACGAGCAG CATGGACGAA GACTTTGAGT CTGACTACAA GAAGTACTGT 
GTGCTACAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030101830.1
CDS	245..4936
Translation	MSQHCFIREVLGDGVPPKVAEVIYCSFGGTSKGLHFNNLIVGLVLLTRGKDEEKAKYIFSLFSSESGSYVVREEMERMLHVVDGKVPDTLRKCFSEGEKVNYEKFRNWLLLNKDAFTFSRWLLSGGVYVTLTDDSDTPTFYQTLAGVTHLEESDIIDLEKRYWLLKAQSRTGRFDLETFGPLVSPPIRPSLSEGLFNAFDENRDNHIDFKEISCGLSACCRGPLAERQKFCFKVFDVDRDGVLSRVELKDMVVALLEVWKDNRTDDIPELHMDLSDIVERILNAHDTTKVGHLTLEDYQIWSVKNVLANEFLNLLFQVCHIVLGLRPATPEEEGQIIRGWLERESRYGLQPGHNWFIISMQWWQQWKEYVKYDASPVVIEPSSVLNGGKFSFGTAAHPIEHGEDRISNNLGYMNTTEEKYSDNISSASEASESTGSGFLYSGTPGADMCFARQHNTSDNNNQCLLGANGNILLHLNPQKPGAIDNQPLVTQEPVKATSLTLEGGRLKRTPQLIHGRDYEMVPEPVWRALYHWYGSNLALPRPVIKNSKTDIPELELFPRYLLFLRQQPATRTQQSNIWVNMGNVPSPNAPLKRVLAYTGCFSRMQTIKEIHEYLSQRLRIKEEDMRLWLYNSENYLTLLDDEDHRLEYLKIQDEQHLVIEVRNKDMSWPEEMSFIANSSKIDRHKVPTEKGATGLSNLGNTCFMNSSIQCVSNTQPLTQYFISGRHLYELNRTNPIGMKGHMAKCYGDLVQELWSGTQKNVAPLKLRWTIAKYAPRFNGFQQQDSQELLAFLLDGLHEDLNRVHEKPYVELKDSDGRPDWEVAAEAWDNHLRRNRSIVVDLFHGQLRSQVKCKTCGHISVRFDPFNFLSLPLPMDSYMHLEITVIKLDGTTPIRYGLRLNMDEKYTGLKKQLSDLCGLKSEQILFAEVHSSNIKNFPQDNQKVRLSVSGFLCAFEIPIPASPVSACSPIQTDCSSSPSTNGLFTLTTNGDLPRPIFIPNGMPNTVVPCGTEKNVTNGIVNGHMPPLPDDPFTGYIIAVHRKMMRTELYFLSSQKNRPSLFGMPLIVPCTVHTRKKDLYDAVWIQVSRLASPLPPQEASNHAQDCDDSMGYQYPFTLRVVQKDGNSCAWCPWYRFCRGCKIDCGEDRAFIGNACIAVDWDPTALHLRYQTSQERVVEEHESVEQSRRAQAEPINLDSCLRAFTSEEELGENEMYYCSKCKTHCLATKKLDLWRLPPILIIHLKRFQFVNGRWIKSQKIVKFPRESFDPSAFLVPRDPTLCQHKPLTPQGDDFSELRIPAGDVKKVDIQSSAGEEDVLLSKSPSSLSANVTSSPKGSPSSSRKSGASCPSSKNSSPNSSPRTLGRNKGRLRLPQIGSKNKLSNSKENLDTSKENGAGQICELADTLNRRHVLGGSQPELVTPLDHEITLANGFLYEHEACGNGYSNGQLGNHSEEDSTDDQREETHSKPIYNLYAISCHSGILGGGHYVTYAKNPNCKWYCYNDSSCKELHPDEIDTDSAYILFYEQQGIDCAQFLPKTDGKKMADTSSMDEDFESDYKKYCVLQ

Target ORF information:

RefSeq Version	XM_030245970.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin specific peptidase 32 (Usp32), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030245970.1

ORF Insert Sequence:

ATGAGTCAGC ACTGCTTCAT CAGGGAAGTG CTTGGGGATG GAGTACCTCC TAAAGTTGCT 
GAGGTGATTT ACTGTTCTTT TGGTGGAACA TCCAAAGGGC TGCATTTCAA TAACTTAATA 
GTTGGACTCG TCCTCCTTAC AAGAGGCAAA GATGAAGAGA AAGCAAAATA TATTTTTAGT 
CTTTTTTCAA GTGAATCCGG GAGCTATGTT GTACGGGAAG AAATGGAGAG AATGCTCCAT 
GTGGTGGATG GTAAAGTCCC AGATACACTC AGGAAGTGTT TCTCAGAGGG TGAAAAGGTA 
AACTATGAAA AATTCAGAAA TTGGCTTCTT CTAAACAAAG ATGCTTTTAC CTTCTCTCGC 
TGGCTGTTGT CTGGGGGTGT ATATGTGACC CTCACTGATG ACAGTGACAC TCCTACTTTC 
TACCAAACTC TGGCTGGAGT CACACATTTG GAAGAGTCAG ACATCATCGA TCTGGAGAAA 
CGGTATTGGC TACTGAAGGC CCAATCCCGA ACTGGACGTT TTGACTTAGA GACCTTTGGT 
CCATTGGTTT CACCACCTAT TCGCCCTTCT CTGAGTGAAG GATTATTTAA TGCTTTTGAT 
GAAAATCGTG ACAATCACAT AGACTTCAAG GAGATATCCT GTGGGTTATC AGCCTGCTGC 
AGGGGCCCCC TGGCTGAAAG ACAAAAATTT TGCTTCAAGG TGTTTGATGT TGACCGTGAT 
GGAGTTCTCT CCAGGGTTGA ACTGAAAGAC ATGGTAGTTG CACTTTTGGA GGTCTGGAAA 
GATAACCGCA CTGATGATAT TCCTGAATTG CATATGGATT TGTCAGACAT TGTAGAAAGG 
ATATTGAATG CACATGATAC CACAAAGGTG GGCCATCTTA CTCTTGAAGA CTATCAGATA 
TGGAGTGTGA AAAATGTTCT TGCCAATGAG TTTTTGAATC TCCTCTTCCA GGTATGCCAC 
ATAGTTCTGG GGTTAAGACC AGCTACTCCA GAAGAAGAAG GACAAATTAT TAGAGGATGG 
TTGGAACGAG AAAGCAGGTA TGGTCTGCAG CCAGGACACA ATTGGTTTAT CATCTCCATG 
CAGTGGTGGC AACAGTGGAA AGAATATGTC AAATATGATG CCAGCCCTGT TGTAATTGAG 
CCATCATCTG TTCTAAATGG AGGAAAATTT TCATTTGGAA CAGCAGCACA TCCTATAGAG 
CATGGAGAAG ATAGAATCAG TAATAACCTT GGTTATATGA ATACCACAGA AGAGAAATAC 
TCAGACAACA TTTCTTCTGC ATCTGAAGCC TCAGAGTCTA CTGGCAGTGG CTTTCTGTAT 
TCTGGCACAC CAGGGGCAGA TATGTGCTTT GCAAGGCAGC ATAACACTTC TGACAATAAC 
AACCAGTGTT TACTAGGAGC CAATGGGAAT ATCCTTTTGC ACCTTAATCC TCAGAAGCCA 
GGAGCAATTG ATAACCAGCC ACTAGTAACT CAGGAACCAG TAAAGGCTAC ATCATTAACA 
CTAGAAGGAG GGCGATTAAA ACGAACTCCA CAGCTAATCC ATGGAAGAGA TTATGAAATG 
GTCCCAGAAC CCGTGTGGAG AGCACTTTAT CATTGGTATG GATCAAACCT GGCCCTTCCT 
AGACCAGTAA TCAAGAATAG CAAAACGGAC ATCCCAGAAC TAGAATTATT TCCTCGCTAT 
CTTCTCTTCC TTCGACAGCA GCCTGCCACA CGGACACAGC AATCTAACAT CTGGGTGAAT 
ATGGGAAATG TACCCTCTCC AAATGCGCCT CTAAAGCGAG TGTTAGCCTA TACAGGCTGT 
TTTAGTAGAA TGCAAACCAT CAAGGAAATT CATGAATATC TGTCTCAAAG GCTTCGCATT 
AAAGAGGAAG ATATGCGGCT GTGGCTGTAC AATAGTGAGA ACTACCTGAC TCTTCTGGAT 
GATGAGGATC ACAGATTGGA ATATTTGAAA ATCCAGGATG AGCAGCACCT GGTAATTGAA 
GTTCGAAACA AAGATATGAG TTGGCCTGAA GAAATGTCTT TTATAGCAAA CAGTAGCAAA 
ATAGATAGAC ATAAAGTTCC CACAGAAAAG GGCGCCACAG GTCTGAGTAA CCTGGGAAAC 
ACATGCTTCA TGAACTCAAG CATCCAGTGT GTCAGCAATA CACAACCACT GACACAGTAC 
TTTATCTCAG GGAGGCATCT TTATGAACTC AACAGGACAA ATCCCATTGG TATGAAAGGC 
CATATGGCTA AATGTTATGG CGATTTGGTG CAAGAACTTT GGAGTGGAAC TCAGAAGAAT 
GTTGCCCCCT TAAAGCTCCG GTGGACCATA GCAAAATATG CTCCCAGGTT TAATGGATTC 
CAGCAACAAG ACTCCCAAGA ACTTCTGGCT TTCCTCTTGG ATGGTCTTCA TGAAGATCTC 
AACCGAGTCC ACGAGAAACC ATATGTAGAA CTAAAAGACA GTGACGGCCG ACCCGACTGG 
GAAGTAGCTG CAGAGGCCTG GGACAACCAT CTAAGGAGAA ATAGATCAAT TGTTGTGGAC 
TTGTTCCATG GTCAGCTAAG ATCCCAAGTC AAATGCAAGA CATGTGGGCA TATAAGTGTC 
CGATTCGACC CTTTTAATTT TTTGTCTTTA CCACTACCAA TGGACAGTTA CATGCATTTA 
GAAATAACAG TAATTAAATT AGATGGTACT ACCCCCATAC GATATGGACT AAGACTGAAT 
ATGGATGAAA AGTATACAGG TTTGAAAAAG CAGCTGAGTG ATCTCTGTGG ACTTAAATCA 
GAACAAATCC TTTTTGCAGA AGTGCATAGT TCAAACATAA AGAACTTCCC TCAGGACAAC 
CAAAAAGTTC GACTCTCAGT GAGTGGATTT TTGTGTGCAT TTGAAATTCC TATTCCTGCA 
TCTCCAGTTT CAGCTTGCAG TCCAATTCAA ACAGATTGCT CTTCTTCTCC ATCTACAAAT 
GGACTGTTTA CCTTGACTAC CAATGGGGAT CTGCCTCGAC CTATATTCAT CCCCAATGGA 
ATGCCAAATA CAGTTGTACC ATGTGGAACT GAGAAGAATG TCACAAATGG AATAGTGAAT 
GGTCATATGC CGCCTCTTCC TGATGACCCC TTTACAGGCT ACATCATTGC AGTCCACCGA 
AAAATGATGA GGACAGAACT GTATTTCCTG TCATCTCAGA AGAATCGCCC CAGTCTCTTT 
GGGATGCCAC TGATTGTTCC ATGTACTGTA CATACCCGGA AAAAAGATCT CTATGATGCA 
GTTTGGATTC AAGTATCTCG ATTAGCCAGT CCGCTCCCAC CTCAAGAAGC TAGCAACCAT 
GCACAGGATT GTGATGACAG CATGGGCTAT CAATATCCAT TCACTCTCCG AGTTGTGCAG 
AAAGATGGAA ACTCCTGTGC CTGGTGTCCA TGGTATAGAT TTTGCAGAGG CTGTAAAATC 
GATTGTGGAG AAGACAGAGC TTTCATTGGA AATGCCTGTA TTGCTGTGGA TTGGGACCCT 
ACAGCCCTTC ACCTCCGCTA TCAAACATCA CAGGAAAGAG TTGTAGAGGA GCATGAAAGT 
GTGGAACAGA GTCGGCGAGC ACAAGCTGAG CCCATCAACC TGGACAGCTG TCTCCGTGCC 
TTCACCAGTG AGGAGGAACT GGGGGAAAAT GAGATGTACT ACTGCTCCAA ATGTAAAACA 
CACTGTTTGG CAACCAAGAA ACTGGACCTC TGGAGGCTTC CTCCAATCCT GATTATTCAC 
CTTAAACGAT TTCAGTTTGT AAATGGACGG TGGATAAAAT CACAGAAAAT TGTTAAATTT 
CCTCGTGAAA GTTTTGACCC TAGTGCTTTT TTAGTACCAA GAGACCCAAC TCTTTGCCAG 
CATAAACCAC TCACACCCCA GGGGGATGAC TTCTCTGAGC TAAGGATCCC AGCAGGAGAT 
GTGAAGAAAG TGGATATCCA GAGCTCAGCA GGGGAGGAGG ATGTGCTCCT GAGCAAGAGC 
CCATCTTCAC TCAGTGCAAA TGTCACCAGT AGCCCAAAAG GTTCACCTTC TTCATCAAGA 
AAAAGTGGAG CCAGCTGTCC CTCCAGCAAA AATAGCAGCC CTAACAGCAG CCCACGGACC 
TTGGGGAGGA ACAAAGGGAG GCTCCGGCTG CCTCAAATTG GCAGCAAAAA TAAATTGTCC 
AATAGTAAGG AGAATTTGGA TACCAGCAAA GAGAATGGGG CTGGGCAGAT CTGTGAACTG 
GCTGACACCT TGAACCGAAG ACATGTATTG GGAGGCAGCC AGCCTGAGCT GGTCACTCCT 
CTGGATCATG AGATCACTTT GGCTAATGGA TTTCTTTATG AGCATGAGGC ATGTGGCAAT 
GGCTATAGCA ATGGTCAGCT TGGAAACCAT AGTGAAGAAG ACAGCACTGA TGACCAAAGA 
GAAGAAACAC ACAGTAAACC TATTTATAAT CTATATGCAA TTTCGTGCCA TTCAGGAATT 
CTGGGTGGGG GCCATTACGT CACTTATGCC AAAAACCCAA ACTGCAAGTG GTACTGTTAC 
AATGACAGCA GCTGTAAGGA GCTTCACCCT GATGAGATAG ACACCGACTC TGCCTACATT 
CTTTTCTATG AGCAGCAGGG GATAGACTGT GCACAGTTTC TGCCAAAGAC AGACGGCAAG 
AAGATGGCGG ACACGAGCAG CATGGACGAA GACTTTGAGT CTGACTACAA GAAGTACTGT 
GTGCTACAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Usp32 ( NM_001029934.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001025105.1 Mus musculus ubiquitin specific peptidase 32 (Usp32), mRNA.

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CloneID	OMu11470
Clone ID Related Accession (Same CDS sequence)	NM_001029934.1
Accession Version	NM_001029934.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-09
Organism	Mus musculus(house mouse)
Product	ubiquitin carboxyl-terminal hydrolase 32
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL596183.11, AL669859.5 and BC058994.1. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN01164139 [ECO:0000348] ##Evidence-Data-END##

ATGGGCGCCA AGGAGTCACG GATCGGATTC CTCAGCTACG AGGAGGCGCT GAGGAGAGTT 
ACAGATGTTG AGCTGAAACG ACTCAAAGAT GCCTTCAAGA GGACCTGCGG CCTCTCCTAT 
TACATGAGTC AGCACTGCTT CATCAGGGAA GTGCTTGGGG ATGGAGTACC TCCTAAAGTT 
GCTGAGGTGA TTTACTGTTC TTTTGGTGGA ACATCCAAAG GGCTGCATTT CAATAACTTA 
ATAGTTGGAC TCGTCCTCCT TACAAGAGGC AAAGATGAAG AGAAAGCAAA ATATATTTTT 
AGTCTTTTTT CAAGTGAATC CGGGAGCTAT GTTGTACGGG AAGAAATGGA GAGAATGCTC 
CATGTGGTGG ATGGTAAAGT CCCAGATACA CTCAGGAAGT GTTTCTCAGA GGGTGAAAAG 
GTAAACTATG AAAAATTCAG AAATTGGCTT CTTCTAAACA AAGATGCTTT TACCTTCTCT 
CGCTGGCTGT TGTCTGGGGG TGTATATGTG ACCCTCACTG ATGACAGTGA CACTCCTACT 
TTCTACCAAA CTCTGGCTGG AGTCACACAT TTGGAAGAGT CAGACATCAT CGATCTGGAG 
AAACGGTATT GGCTACTGAA GGCCCAATCC CGAACTGGAC GTTTTGACTT AGAGACCTTT 
GGTCCATTGG TTTCACCACC TATTCGCCCT TCTCTGAGTG AAGGATTATT TAATGCTTTT 
GATGAAAATC GTGACAATCA CATAGACTTC AAGGAGATAT CCTGTGGGTT ATCAGCCTGC 
TGCAGGGGCC CCCTGGCTGA AAGACAAAAA TTTTGCTTCA AGGTGTTTGA TGTTGACCGT 
GATGGAGTTC TCTCCAGGGT TGAACTGAAA GACATGGTAG TTGCACTTTT GGAGGTCTGG 
AAAGATAACC GCACTGATGA TATTCCTGAA TTGCATATGG ATTTGTCAGA CATTGTAGAA 
AGGATATTGA ATGCACATGA TACCACAAAG GTGGGCCATC TTACTCTTGA AGACTATCAG 
ATATGGAGTG TGAAAAATGT TCTTGCCAAT GAGTTTTTGA ATCTCCTCTT CCAGGTATGC 
CACATAGTTC TGGGGTTAAG ACCAGCTACT CCAGAAGAAG AAGGACAAAT TATTAGAGGA 
TGGTTGGAAC GAGAAAGCAG GTATGGTCTG CAGCCAGGAC ACAATTGGTT TATCATCTCC 
ATGCAGTGGT GGCAACAGTG GAAAGAATAT GTCAAATATG ATGCCAGCCC TGTTGTAATT 
GAGCCATCAT CTGTTCTAAA TGGAGGAAAA TTTTCATTTG GAACAGCAGC ACATCCTATA 
GAGCATGGAG AAGATAGAAT CAGTAATAAC CTTGGTTATA TGAATACCAC AGAAGAGAAA 
TACTCAGACA ACATTTCTTC TGCATCTGAA GCCTCAGAGT CTACTGGCAG TGGCTTTCTG 
TATTCTGGCA CACCAGGGGC AGATATGTGC TTTGCAAGGC AGCATAACAC TTCTGACAAT 
AACAACCAGT GTTTACTAGG AGCCAATGGG AATATCCTTT TGCACCTTAA TCCTCAGAAG 
CCAGGAGCAA TTGATAACCA GCCACTAGTA ACTCAGGAAC CAGTAAAGGC TACATCATTA 
ACACTAGAAG GAGGGCGATT AAAACGAACT CCACAGCTAA TCCATGGAAG AGATTATGAA 
ATGGTCCCAG AACCCGTGTG GAGAGCACTT TATCATTGGT ATGGATCAAA CCTGGCCCTT 
CCTAGACCAG TAATCAAGAA TAGCAAAACG GACATCCCAG AACTAGAATT ATTTCCTCGC 
TATCTTCTCT TCCTTCGACA GCAGCCTGCC ACACGGACAC AGCAATCTAA CATCTGGGTG 
AATATGGGAA ATGTACCCTC TCCAAATGCG CCTCTAAAGC GAGTGTTAGC CTATACAGGC 
TGTTTTAGTA GAATGCAAAC CATCAAGGAA ATTCATGAAT ATCTGTCTCA AAGGCTTCGC 
ATTAAAGAGG AAGATATGCG GCTGTGGCTG TACAATAGTG AGAACTACCT GACTCTTCTG 
GATGATGAGG ATCACAGATT GGAATATTTG AAAATCCAGG ATGAGCAGCA CCTGGTAATT 
GAAGTTCGAA ACAAAGATAT GAGTTGGCCT GAAGAAATGT CTTTTATAGC AAACAGTAGC 
AAAATAGATA GACATAAAGT TCCCACAGAA AAGGGCGCCA CAGGTCTGAG TAACCTGGGA 
AACACATGCT TCATGAACTC AAGCATCCAG TGTGTCAGCA ATACACAACC ACTGACACAG 
TACTTTATCT CAGGGAGGCA TCTTTATGAA CTCAACAGGA CAAATCCCAT TGGTATGAAA 
GGCCATATGG CTAAATGTTA TGGCGATTTG GTGCAAGAAC TTTGGAGTGG AACTCAGAAG 
AATGTTGCCC CCTTAAAGCT CCGGTGGACC ATAGCAAAAT ATGCTCCCAG GTTTAATGGA 
TTCCAGCAAC AAGACTCCCA AGAACTTCTG GCTTTCCTCT TGGATGGTCT TCATGAAGAT 
CTCAACCGAG TCCACGAGAA ACCATATGTA GAACTAAAAG ACAGTGACGG CCGACCCGAC 
TGGGAAGTAG CTGCAGAGGC CTGGGACAAC CATCTAAGGA GAAATAGATC AATTGTTGTG 
GACTTGTTCC ATGGTCAGCT AAGATCCCAA GTCAAATGCA AGACATGTGG GCATATAAGT 
GTCCGATTCG ACCCTTTTAA TTTTTTGTCT TTACCACTAC CAATGGACAG TTACATGCAT 
TTAGAAATAA CAGTAATTAA ATTAGATGGT ACTACCCCCA TACGATATGG ACTAAGACTG 
AATATGGATG AAAAGTATAC AGGTTTGAAA AAGCAGCTGA GTGATCTCTG TGGACTTAAA 
TCAGAACAAA TCCTTTTTGC AGAAGTGCAT AGTTCAAACA TAAAGAACTT CCCTCAGGAC 
AACCAAAAAG TTCGACTCTC AGTGAGTGGA TTTTTGTGTG CATTTGAAAT TCCTATTCCT 
GCATCTCCAG TTTCAGCTTG CAGTCCAATT CAAACAGATT GCTCTTCTTC TCCATCTACA 
AATGGACTGT TTACCTTGAC TACCAATGGG GATCTGCCTC GACCTATATT CATCCCCAAT 
GGAATGCCAA ATACAGTTGT ACCATGTGGA ACTGAGAAGA ATGTCACAAA TGGAATAGTG 
AATGGTCATA TGCCGCCTCT TCCTGATGAC CCCTTTACAG GCTACATCAT TGCAGTCCAC 
CGAAAAATGA TGAGGACAGA ACTGTATTTC CTGTCATCTC AGAAGAATCG CCCCAGTCTC 
TTTGGGATGC CACTGATTGT TCCATGTACT GTACATACCC GGAAAAAAGA TCTCTATGAT 
GCAGTTTGGA TTCAAGTATC TCGATTAGCC AGTCCGCTCC CACCTCAAGA AGCTAGCAAC 
CATGCACAGG ATTGTGATGA CAGCATGGGC TATCAATATC CATTCACTCT CCGAGTTGTG 
CAGAAAGATG GAAACTCCTG TGCCTGGTGT CCATGGTATA GATTTTGCAG AGGCTGTAAA 
ATCGATTGTG GAGAAGACAG AGCTTTCATT GGAAATGCCT GTATTGCTGT GGATTGGGAC 
CCTACAGCCC TTCACCTCCG CTATCAAACA TCACAGGAAA GAGTTGTAGA GGAGCATGAA 
AGTGTGGAAC AGAGTCGGCG AGCACAAGCT GAGCCCATCA ACCTGGACAG CTGTCTCCGT 
GCCTTCACCA GTGAGGAGGA ACTGGGGGAA AATGAGATGT ACTACTGCTC CAAATGTAAA 
ACACACTGTT TGGCAACCAA GAAACTGGAC CTCTGGAGGC TTCCTCCAAT CCTGATTATT 
CACCTTAAAC GATTTCAGTT TGTAAATGGA CGGTGGATAA AATCACAGAA AATTGTTAAA 
TTTCCTCGTG AAAGTTTTGA CCCTAGTGCT TTTTTAGTAC CAAGAGACCC AACTCTTTGC 
CAGCATAAAC CACTCACACC CCAGGGGGAT GACTTCTCTG AGCTAAGGAT CCCAGCAGGA 
GATGTGAAGA AAGTGGATAT CCAGAGCTCA GCAGGGGAGG AGGATGTGCT CCTGAGCAAG 
AGCCCATCTT CACTCAGTGC AAATGTCACC AGTAGCCCAA AAGGTTCACC TTCTTCATCA 
AGAAAAAGTG GAGCCAGCTG TCCCTCCAGC AAAAATAGCA GCCCTAACAG CAGCCCACGG 
ACCTTGGGGA GGAACAAAGG GAGGCTCCGG CTGCCTCAAA TTGGCAGCAA AAATAAATTG 
TCCAATAGTA AGGAGAATTT GGATACCAGC AAAGAGAATG GGGCTGGGCA GATCTGTGAA 
CTGGCTGACA CCTTGAACCG AAGACATGTA TTGGGAGGCA GCCAGCCTGA GCTGGTCACT 
CCTCTGGATC ATGAGATCAC TTTGGCTAAT GGATTTCTTT ATGAGCATGA GGCATGTGGC 
AATGGCTATA GCAATGGTCA GCTTGGAAAC CATAGTGAAG AAGACAGCAC TGATGACCAA 
AGAGAAGAAA CACACAGTAA ACCTATTTAT AATCTATATG CAATTTCGTG CCATTCAGGA 
ATTCTGGGTG GGGGCCATTA CGTCACTTAT GCCAAAAACC CAAACTGCAA GTGGTACTGT 
TACAATGACA GCAGCTGTAA GGAGCTTCAC CCTGATGAGA TAGACACCGA CTCTGCCTAC 
ATTCTTTTCT ATGAGCAGCA GGGGATAGAC TGTGCACAGT TTCTGCCAAA GACAGACGGC 
AAGAAGATGG CGGACACGAG CAGCATGGAC GAAGACTTTG AGTCTGACTA CAAGAAGTAC 
TGTGTGCTAC AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001025105.1
CDS	188..5002
Translation	MGAKESRIGFLSYEEALRRVTDVELKRLKDAFKRTCGLSYYMSQHCFIREVLGDGVPPKVAEVIYCSFGGTSKGLHFNNLIVGLVLLTRGKDEEKAKYIFSLFSSESGSYVVREEMERMLHVVDGKVPDTLRKCFSEGEKVNYEKFRNWLLLNKDAFTFSRWLLSGGVYVTLTDDSDTPTFYQTLAGVTHLEESDIIDLEKRYWLLKAQSRTGRFDLETFGPLVSPPIRPSLSEGLFNAFDENRDNHIDFKEISCGLSACCRGPLAERQKFCFKVFDVDRDGVLSRVELKDMVVALLEVWKDNRTDDIPELHMDLSDIVERILNAHDTTKVGHLTLEDYQIWSVKNVLANEFLNLLFQVCHIVLGLRPATPEEEGQIIRGWLERESRYGLQPGHNWFIISMQWWQQWKEYVKYDASPVVIEPSSVLNGGKFSFGTAAHPIEHGEDRISNNLGYMNTTEEKYSDNISSASEASESTGSGFLYSGTPGADMCFARQHNTSDNNNQCLLGANGNILLHLNPQKPGAIDNQPLVTQEPVKATSLTLEGGRLKRTPQLIHGRDYEMVPEPVWRALYHWYGSNLALPRPVIKNSKTDIPELELFPRYLLFLRQQPATRTQQSNIWVNMGNVPSPNAPLKRVLAYTGCFSRMQTIKEIHEYLSQRLRIKEEDMRLWLYNSENYLTLLDDEDHRLEYLKIQDEQHLVIEVRNKDMSWPEEMSFIANSSKIDRHKVPTEKGATGLSNLGNTCFMNSSIQCVSNTQPLTQYFISGRHLYELNRTNPIGMKGHMAKCYGDLVQELWSGTQKNVAPLKLRWTIAKYAPRFNGFQQQDSQELLAFLLDGLHEDLNRVHEKPYVELKDSDGRPDWEVAAEAWDNHLRRNRSIVVDLFHGQLRSQVKCKTCGHISVRFDPFNFLSLPLPMDSYMHLEITVIKLDGTTPIRYGLRLNMDEKYTGLKKQLSDLCGLKSEQILFAEVHSSNIKNFPQDNQKVRLSVSGFLCAFEIPIPASPVSACSPIQTDCSSSPSTNGLFTLTTNGDLPRPIFIPNGMPNTVVPCGTEKNVTNGIVNGHMPPLPDDPFTGYIIAVHRKMMRTELYFLSSQKNRPSLFGMPLIVPCTVHTRKKDLYDAVWIQVSRLASPLPPQEASNHAQDCDDSMGYQYPFTLRVVQKDGNSCAWCPWYRFCRGCKIDCGEDRAFIGNACIAVDWDPTALHLRYQTSQERVVEEHESVEQSRRAQAEPINLDSCLRAFTSEEELGENEMYYCSKCKTHCLATKKLDLWRLPPILIIHLKRFQFVNGRWIKSQKIVKFPRESFDPSAFLVPRDPTLCQHKPLTPQGDDFSELRIPAGDVKKVDIQSSAGEEDVLLSKSPSSLSANVTSSPKGSPSSSRKSGASCPSSKNSSPNSSPRTLGRNKGRLRLPQIGSKNKLSNSKENLDTSKENGAGQICELADTLNRRHVLGGSQPELVTPLDHEITLANGFLYEHEACGNGYSNGQLGNHSEEDSTDDQREETHSKPIYNLYAISCHSGILGGGHYVTYAKNPNCKWYCYNDSSCKELHPDEIDTDSAYILFYEQQGIDCAQFLPKTDGKKMADTSSMDEDFESDYKKYCVLQ

Target ORF information:

RefSeq Version	NM_001029934.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin specific peptidase 32 (Usp32), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001029934.1

ORF Insert Sequence:

ATGGGCGCCA AGGAGTCACG GATCGGATTC CTCAGCTACG AGGAGGCGCT GAGGAGAGTT 
ACAGATGTTG AGCTGAAACG ACTCAAAGAT GCCTTCAAGA GGACCTGCGG CCTCTCCTAT 
TACATGAGTC AGCACTGCTT CATCAGGGAA GTGCTTGGGG ATGGAGTACC TCCTAAAGTT 
GCTGAGGTGA TTTACTGTTC TTTTGGTGGA ACATCCAAAG GGCTGCATTT CAATAACTTA 
ATAGTTGGAC TCGTCCTCCT TACAAGAGGC AAAGATGAAG AGAAAGCAAA ATATATTTTT 
AGTCTTTTTT CAAGTGAATC CGGGAGCTAT GTTGTACGGG AAGAAATGGA GAGAATGCTC 
CATGTGGTGG ATGGTAAAGT CCCAGATACA CTCAGGAAGT GTTTCTCAGA GGGTGAAAAG 
GTAAACTATG AAAAATTCAG AAATTGGCTT CTTCTAAACA AAGATGCTTT TACCTTCTCT 
CGCTGGCTGT TGTCTGGGGG TGTATATGTG ACCCTCACTG ATGACAGTGA CACTCCTACT 
TTCTACCAAA CTCTGGCTGG AGTCACACAT TTGGAAGAGT CAGACATCAT CGATCTGGAG 
AAACGGTATT GGCTACTGAA GGCCCAATCC CGAACTGGAC GTTTTGACTT AGAGACCTTT 
GGTCCATTGG TTTCACCACC TATTCGCCCT TCTCTGAGTG AAGGATTATT TAATGCTTTT 
GATGAAAATC GTGACAATCA CATAGACTTC AAGGAGATAT CCTGTGGGTT ATCAGCCTGC 
TGCAGGGGCC CCCTGGCTGA AAGACAAAAA TTTTGCTTCA AGGTGTTTGA TGTTGACCGT 
GATGGAGTTC TCTCCAGGGT TGAACTGAAA GACATGGTAG TTGCACTTTT GGAGGTCTGG 
AAAGATAACC GCACTGATGA TATTCCTGAA TTGCATATGG ATTTGTCAGA CATTGTAGAA 
AGGATATTGA ATGCACATGA TACCACAAAG GTGGGCCATC TTACTCTTGA AGACTATCAG 
ATATGGAGTG TGAAAAATGT TCTTGCCAAT GAGTTTTTGA ATCTCCTCTT CCAGGTATGC 
CACATAGTTC TGGGGTTAAG ACCAGCTACT CCAGAAGAAG AAGGACAAAT TATTAGAGGA 
TGGTTGGAAC GAGAAAGCAG GTATGGTCTG CAGCCAGGAC ACAATTGGTT TATCATCTCC 
ATGCAGTGGT GGCAACAGTG GAAAGAATAT GTCAAATATG ATGCCAGCCC TGTTGTAATT 
GAGCCATCAT CTGTTCTAAA TGGAGGAAAA TTTTCATTTG GAACAGCAGC ACATCCTATA 
GAGCATGGAG AAGATAGAAT CAGTAATAAC CTTGGTTATA TGAATACCAC AGAAGAGAAA 
TACTCAGACA ACATTTCTTC TGCATCTGAA GCCTCAGAGT CTACTGGCAG TGGCTTTCTG 
TATTCTGGCA CACCAGGGGC AGATATGTGC TTTGCAAGGC AGCATAACAC TTCTGACAAT 
AACAACCAGT GTTTACTAGG AGCCAATGGG AATATCCTTT TGCACCTTAA TCCTCAGAAG 
CCAGGAGCAA TTGATAACCA GCCACTAGTA ACTCAGGAAC CAGTAAAGGC TACATCATTA 
ACACTAGAAG GAGGGCGATT AAAACGAACT CCACAGCTAA TCCATGGAAG AGATTATGAA 
ATGGTCCCAG AACCCGTGTG GAGAGCACTT TATCATTGGT ATGGATCAAA CCTGGCCCTT 
CCTAGACCAG TAATCAAGAA TAGCAAAACG GACATCCCAG AACTAGAATT ATTTCCTCGC 
TATCTTCTCT TCCTTCGACA GCAGCCTGCC ACACGGACAC AGCAATCTAA CATCTGGGTG 
AATATGGGAA ATGTACCCTC TCCAAATGCG CCTCTAAAGC GAGTGTTAGC CTATACAGGC 
TGTTTTAGTA GAATGCAAAC CATCAAGGAA ATTCATGAAT ATCTGTCTCA AAGGCTTCGC 
ATTAAAGAGG AAGATATGCG GCTGTGGCTG TACAATAGTG AGAACTACCT GACTCTTCTG 
GATGATGAGG ATCACAGATT GGAATATTTG AAAATCCAGG ATGAGCAGCA CCTGGTAATT 
GAAGTTCGAA ACAAAGATAT GAGTTGGCCT GAAGAAATGT CTTTTATAGC AAACAGTAGC 
AAAATAGATA GACATAAAGT TCCCACAGAA AAGGGCGCCA CAGGTCTGAG TAACCTGGGA 
AACACATGCT TCATGAACTC AAGCATCCAG TGTGTCAGCA ATACACAACC ACTGACACAG 
TACTTTATCT CAGGGAGGCA TCTTTATGAA CTCAACAGGA CAAATCCCAT TGGTATGAAA 
GGCCATATGG CTAAATGTTA TGGCGATTTG GTGCAAGAAC TTTGGAGTGG AACTCAGAAG 
AATGTTGCCC CCTTAAAGCT CCGGTGGACC ATAGCAAAAT ATGCTCCCAG GTTTAATGGA 
TTCCAGCAAC AAGACTCCCA AGAACTTCTG GCTTTCCTCT TGGATGGTCT TCATGAAGAT 
CTCAACCGAG TCCACGAGAA ACCATATGTA GAACTAAAAG ACAGTGACGG CCGACCCGAC 
TGGGAAGTAG CTGCAGAGGC CTGGGACAAC CATCTAAGGA GAAATAGATC AATTGTTGTG 
GACTTGTTCC ATGGTCAGCT AAGATCCCAA GTCAAATGCA AGACATGTGG GCATATAAGT 
GTCCGATTCG ACCCTTTTAA TTTTTTGTCT TTACCACTAC CAATGGACAG TTACATGCAT 
TTAGAAATAA CAGTAATTAA ATTAGATGGT ACTACCCCCA TACGATATGG ACTAAGACTG 
AATATGGATG AAAAGTATAC AGGTTTGAAA AAGCAGCTGA GTGATCTCTG TGGACTTAAA 
TCAGAACAAA TCCTTTTTGC AGAAGTGCAT AGTTCAAACA TAAAGAACTT CCCTCAGGAC 
AACCAAAAAG TTCGACTCTC AGTGAGTGGA TTTTTGTGTG CATTTGAAAT TCCTATTCCT 
GCATCTCCAG TTTCAGCTTG CAGTCCAATT CAAACAGATT GCTCTTCTTC TCCATCTACA 
AATGGACTGT TTACCTTGAC TACCAATGGG GATCTGCCTC GACCTATATT CATCCCCAAT 
GGAATGCCAA ATACAGTTGT ACCATGTGGA ACTGAGAAGA ATGTCACAAA TGGAATAGTG 
AATGGTCATA TGCCGCCTCT TCCTGATGAC CCCTTTACAG GCTACATCAT TGCAGTCCAC 
CGAAAAATGA TGAGGACAGA ACTGTATTTC CTGTCATCTC AGAAGAATCG CCCCAGTCTC 
TTTGGGATGC CACTGATTGT TCCATGTACT GTACATACCC GGAAAAAAGA TCTCTATGAT 
GCAGTTTGGA TTCAAGTATC TCGATTAGCC AGTCCGCTCC CACCTCAAGA AGCTAGCAAC 
CATGCACAGG ATTGTGATGA CAGCATGGGC TATCAATATC CATTCACTCT CCGAGTTGTG 
CAGAAAGATG GAAACTCCTG TGCCTGGTGT CCATGGTATA GATTTTGCAG AGGCTGTAAA 
ATCGATTGTG GAGAAGACAG AGCTTTCATT GGAAATGCCT GTATTGCTGT GGATTGGGAC 
CCTACAGCCC TTCACCTCCG CTATCAAACA TCACAGGAAA GAGTTGTAGA GGAGCATGAA 
AGTGTGGAAC AGAGTCGGCG AGCACAAGCT GAGCCCATCA ACCTGGACAG CTGTCTCCGT 
GCCTTCACCA GTGAGGAGGA ACTGGGGGAA AATGAGATGT ACTACTGCTC CAAATGTAAA 
ACACACTGTT TGGCAACCAA GAAACTGGAC CTCTGGAGGC TTCCTCCAAT CCTGATTATT 
CACCTTAAAC GATTTCAGTT TGTAAATGGA CGGTGGATAA AATCACAGAA AATTGTTAAA 
TTTCCTCGTG AAAGTTTTGA CCCTAGTGCT TTTTTAGTAC CAAGAGACCC AACTCTTTGC 
CAGCATAAAC CACTCACACC CCAGGGGGAT GACTTCTCTG AGCTAAGGAT CCCAGCAGGA 
GATGTGAAGA AAGTGGATAT CCAGAGCTCA GCAGGGGAGG AGGATGTGCT CCTGAGCAAG 
AGCCCATCTT CACTCAGTGC AAATGTCACC AGTAGCCCAA AAGGTTCACC TTCTTCATCA 
AGAAAAAGTG GAGCCAGCTG TCCCTCCAGC AAAAATAGCA GCCCTAACAG CAGCCCACGG 
ACCTTGGGGA GGAACAAAGG GAGGCTCCGG CTGCCTCAAA TTGGCAGCAA AAATAAATTG 
TCCAATAGTA AGGAGAATTT GGATACCAGC AAAGAGAATG GGGCTGGGCA GATCTGTGAA 
CTGGCTGACA CCTTGAACCG AAGACATGTA TTGGGAGGCA GCCAGCCTGA GCTGGTCACT 
CCTCTGGATC ATGAGATCAC TTTGGCTAAT GGATTTCTTT ATGAGCATGA GGCATGTGGC 
AATGGCTATA GCAATGGTCA GCTTGGAAAC CATAGTGAAG AAGACAGCAC TGATGACCAA 
AGAGAAGAAA CACACAGTAA ACCTATTTAT AATCTATATG CAATTTCGTG CCATTCAGGA 
ATTCTGGGTG GGGGCCATTA CGTCACTTAT GCCAAAAACC CAAACTGCAA GTGGTACTGT 
TACAATGACA GCAGCTGTAA GGAGCTTCAC CCTGATGAGA TAGACACCGA CTCTGCCTAC 
ATTCTTTTCT ATGAGCAGCA GGGGATAGAC TGTGCACAGT TTCTGCCAAA GACAGACGGC 
AAGAAGATGG CGGACACGAG CAGCATGGAC GAAGACTTTG AGTCTGACTA CAAGAAGTAC 
TGTGTGCTAC AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Usp32 ( XM_011248990.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011247292.1 Mus musculus ubiquitin specific peptidase 32 (Usp32), transcript variant X1, mRNA.

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CloneID	OMu73888
Clone ID Related Accession (Same CDS sequence)	XM_011248990.1
Accession Version	XM_011248990.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4857bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ubiquitin carboxyl-terminal hydrolase 32 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGCCA AGGAGTCACG GATCGGATTC CTCAGCTACG AGGAGGCGCT GAGGAGAGTT 
ACAGATGTTG AGCTGAAACG ACTCAAAGAT GCCTTCAAGA GGACCTGCGG CCTCTCCTAT 
TACATGAGTC AGCACTGCTT CATCAGGGAA GTGCTTGGGG ATGGAGTACC TCCTAAAGTT 
GCTGAGGTGA TTTACTGTTC TTTTGGTGGA ACATCCAAAG GGCTGCATTT CAATAACTTA 
ATAGTTGGAC TCGTCCTCCT TACAAGAGGC AAAGATGAAG AGAAAGCAAA ATATATTTTT 
AGTCTTTTTT CAAGTGAATC CGGGAGCTAT GTTGTACGGG AAGAAATGGA GAGAATGCTC 
CATGTGGTGG ATGGTAAAGT CCCAGATACA CTCAGGAAGT GTTTCTCAGA GGGTGAAAAG 
GTAAACTATG AAAAATTCAG AAATTGGCTT CTTCTAAACA AAGATGCTTT TACCTTCTCT 
CGCTGGCTGT TGTCTGGGGG TGTATATGTG ACCCTCACTG ATGACAGTGA CACTCCTACT 
TTCTACCAAA CTCTGGCTGG AGTCACACAT TTGGAAGAGT CAGACATCAT CGATCTGGAG 
AAACGGTATT GGCTACTGAA GGCCCAATCC CGAACTGGAC GTTTTGACTT AGAGACCTTT 
GGTCCATTGG TTTCACCACC TATTCGCCCT TCTCTGAGTG AAGGATTATT TAATGCTTTT 
GATGAAAATC GTGACAATCA CATAGACTTC AAGGAGATAT CCTGTGGGTT ATCAGCCTGC 
TGCAGGGGCC CCCTGGCTGA AAGACAAAAA TTTTGCTTCA AGGTGTTTGA TGTTGACCGT 
GATGGAGTTC TCTCCAGGGT TGAACTGAAA GACATGGTAG TTGCACTTTT GGAGGTCTGG 
AAAGATAACC GCACTGATGA TATTCCTGAA TTGCATATGG ATTTGTCAGA CATTGTAGAA 
AGGATATTGA ATGCACATGA TACCACAAAG GTGGGCCATC TTACTCTTGA AGACTATCAG 
ATATGGAGTG TGAAAAATGT TCTTGCCAAT GAGTTTTTGA ATCTCCTCTT CCAGGTATGC 
CACATAGTTC TGGGGTTAAG ACCAGCTACT CCAGAAGAAG AAGGACAAAT TATTAGAGGA 
TGGTTGGAAC GAGAAAGCAG GTATGGTCTG CAGCCAGGAC ACAATTGGTT TATCATCTCC 
ATGCAGTGGT GGCAACAGTG GAAAGAATAT GTCAAATATG ATGCCAGCCC TGTTGTAATT 
GAGCCATCAT CTGTTCTAAA TGGAGGAAAA TTTTCATTTG GAACAGCAGC ACATCCTATA 
GAGCATGGAG AAGATAGAAT CAGTAATAAC CTTGGTTATA TGAATACCAC AGAAGAGAAA 
TACTCAGACA ACATTTCTTC TGCATCTGAA GCCTCAGAGT CTACTGGCAG TGGCTTTCTG 
TATTCTGGCA CACCAGGGGC AGATATGTGC TTTGCAAGGC AGCATAACAC TTCTGACAAT 
AACAACCAGT GTTTACTAGG AGCCAATGGG AATATCCTTT TGCACCTTAA TCCTCAGAAG 
CCAGGAGCAA TTGATAACCA GCCACTAGTA ACTCAGGAAC CAGTAAAGGC TACATCATTA 
ACACTAGAAG GAGGGCGATT AAAACGAACT CCACAGCTAA TCCATGGAAG AGATTATGAA 
ATGGTCCCAG AACCCGTGTG GAGAGCACTT TATCATTGGT ATGGATCAAA CCTGGCCCTT 
CCTAGACCAG TAATCAAGAA TAGCAAAACG GACATCCCAG AACTAGAATT ATTTCCTCGC 
TATCTTCTCT TCCTTCGACA GCAGCCTGCC ACACGGACAC AGCAATCTAA CATCTGGGTG 
AATATGGGTA TGATGAGCCT GAGAATGTTT CCTCAGCATT TGCCTAGAGG AAATGTACCC 
TCTCCAAATG CGCCTCTAAA GCGAGTGTTA GCCTATACAG GCTGTTTTAG TAGAATGCAA 
ACCATCAAGG AAATTCATGA ATATCTGTCT CAAAGGCTTC GCATTAAAGA GGAAGATATG 
CGGCTGTGGC TGTACAATAG TGAGAACTAC CTGACTCTTC TGGATGATGA GGATCACAGA 
TTGGAATATT TGAAAATCCA GGATGAGCAG CACCTGGTAA TTGAAGTTCG AAACAAAGAT 
ATGAGTTGGC CTGAAGAAAT GTCTTTTATA GCAAACAGTA GCAAAATAGA TAGACATAAA 
GTTCCCACAG AAAAGGGCGC CACAGGTCTG AGTAACCTGG GAAACACATG CTTCATGAAC 
TCAAGCATCC AGTGTGTCAG CAATACACAA CCACTGACAC AGTACTTTAT CTCAGGGAGG 
CATCTTTATG AACTCAACAG GACAAATCCC ATTGGTATGA AAGGCCATAT GGCTAAATGT 
TATGGCGATT TGGTGCAAGA ACTTTGGAGT GGAACTCAGA AGAATGTTGC CCCCTTAAAG 
CTCCGGTGGA CCATAGCAAA ATATGCTCCC AGGTTTAATG GATTCCAGCA ACAAGACTCC 
CAAGAACTTC TGGCTTTCCT CTTGGATGGT CTTCATGAAG ATCTCAACCG AGTCCACGAG 
AAACCATATG TAGAACTAAA AGACAGTGAC GGCCGACCCG ACTGGGAAGT AGCTGCAGAG 
GCCTGGGACA ACCATCTAAG GAGAAATAGA TCAATTGTTG TGGACTTGTT CCATGGTCAG 
CTAAGATCCC AAGTCAAATG CAAGACATGT GGGCATATAA GTGTCCGATT CGACCCTTTT 
AATTTTTTGT CTTTACCACT ACCAATGGAC AGTTACATGC ATTTAGAAAT AACAGTAATT 
AAATTAGATG GTACTACCCC CATACGATAT GGACTAAGAC TGAATATGGA TGAAAAGTAT 
ACAGGTTTGA AAAAGCAGCT GAGTGATCTC TGTGGACTTA AATCAGAACA AATCCTTTTT 
GCAGAAGTGC ATAGTTCAAA CATAAAGAAC TTCCCTCAGG ACAACCAAAA AGTTCGACTC 
TCAGTGAGTG GATTTTTGTG TGCATTTGAA ATTCCTATTC CTGCATCTCC AGTTTCAGCT 
TGCAGTCCAA TTCAAACAGA TTGCTCTTCT TCTCCATCTA CAAATGGACT GTTTACCTTG 
ACTACCAATG GGGATCTGCC TCGACCTATA TTCATCCCCA ATGGAATGCC AAATACAGTT 
GTACCATGTG GAACTGAGAA GAATGTCACA AATGGAATAG TGAATGGTCA TATGCCGCCT 
CTTCCTGATG ACCCCTTTAC AGGCTACATC ATTGCAGTCC ACCGAAAAAT GATGAGGACA 
GAACTGTATT TCCTGTCATC TCAGAAGAAT CGCCCCAGTC TCTTTGGGAT GCCACTGATT 
GTTCCATGTA CTGTACATAC CCGGAAAAAA GATCTCTATG ATGCAGTTTG GATTCAAGTA 
TCTCGATTAG CCAGTCCGCT CCCACCTCAA GAAGCTAGCA ACCATGCACA GGATTGTGAT 
GACAGCATGG GCTATCAATA TCCATTCACT CTCCGAGTTG TGCAGAAAGA TGGAAACTCC 
TGTGCCTGGT GTCCATGGTA TAGATTTTGC AGAGGCTGTA AAATCGATTG TGGAGAAGAC 
AGAGCTTTCA TTGGAAATGC CTGTATTGCT GTGGATTGGG ACCCTACAGC CCTTCACCTC 
CGCTATCAAA CATCACAGGA AAGAGTTGTA GAGGAGCATG AAAGTGTGGA ACAGAGTCGG 
CGAGCACAAG CTGAGCCCAT CAACCTGGAC AGCTGTCTCC GTGCCTTCAC CAGTGAGGAG 
GAACTGGGGG AAAATGAGAT GTACTACTGC TCCAAATGTA AAACACACTG TTTGGCAACC 
AAGAAACTGG ACCTCTGGAG GCTTCCTCCA ATCCTGATTA TTCACCTTAA ACGATTTCAG 
TTTGTAAATG GACGGTGGAT AAAATCACAG AAAATTGTTA AATTTCCTCG TGAAAGTTTT 
GACCCTAGTG CTTTTTTAGT ACCAAGAGAC CCAACTCTTT GCCAGCATAA ACCACTCACA 
CCCCAGGGGG ATGACTTCTC TGAGCTAAGG ATCCCAGCAG GAGATGTGAA GAAAGTGGAT 
ATCCAGAGCT CAGCAGGGGA GGAGGATGTG CTCCTGAGCA AGAGCCCATC TTCACTCAGT 
GCAAATGTCA CCAGTAGCCC AAAAGGTTCA CCTTCTTCAT CAAGAAAAAG TGGAGCCAGC 
TGTCCCTCCA GCAAAAATAG CAGCCCTAAC AGCAGCCCAC GGACCTTGGG GAGGAACAAA 
GGGAGGCTCC GGCTGCCTCA AATTGGCAGC AAAAATAAAT TGTCCAATAG TAAGGAGAAT 
TTGGATACCA GCAAAGAGAA TGGGGCTGGG CAGATCTGTG AACTGGCTGA CACCTTGAAC 
CGAAGACATG TATTGGGAGG CAGCCAGCCT GAGCTGGTCA CTCCTCTGGA TCATGAGATC 
ACTTTGGCTA ATGGATTTCT TTATGAGCAT GAGGCATGTG GCAATGGCTA TAGCAATGGT 
CAGCTTGGAA ACCATAGTGA AGAAGACAGC ACTGATGACC AAAGAGAAGA AACACACAGT 
AAACCTATTT ATAATCTATA TGCAATTTCG TGCCATTCAG GAATTCTGGG TGGGGGCCAT 
TACGTCACTT ATGCCAAAAA CCCAAACTGC AAGTGGTACT GTTACAATGA CAGCAGCTGT 
AAGGAGCTTC ACCCTGATGA GATAGACACC GACTCTGCCT ACATTCTTTT CTATGAGCAG 
CAGGGGATAG ACTGTGCACA GTTTCTGCCA AAGACAGACG GCAAGAAGAT GGCGGACACG 
AGCAGCATGG ACGAAGACTT TGAGTCTGAC TACAAGAAGT ACTGTGTGCT ACAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011247292.1
CDS	275..5131
Translation	MGAKESRIGFLSYEEALRRVTDVELKRLKDAFKRTCGLSYYMSQHCFIREVLGDGVPPKVAEVIYCSFGGTSKGLHFNNLIVGLVLLTRGKDEEKAKYIFSLFSSESGSYVVREEMERMLHVVDGKVPDTLRKCFSEGEKVNYEKFRNWLLLNKDAFTFSRWLLSGGVYVTLTDDSDTPTFYQTLAGVTHLEESDIIDLEKRYWLLKAQSRTGRFDLETFGPLVSPPIRPSLSEGLFNAFDENRDNHIDFKEISCGLSACCRGPLAERQKFCFKVFDVDRDGVLSRVELKDMVVALLEVWKDNRTDDIPELHMDLSDIVERILNAHDTTKVGHLTLEDYQIWSVKNVLANEFLNLLFQVCHIVLGLRPATPEEEGQIIRGWLERESRYGLQPGHNWFIISMQWWQQWKEYVKYDASPVVIEPSSVLNGGKFSFGTAAHPIEHGEDRISNNLGYMNTTEEKYSDNISSASEASESTGSGFLYSGTPGADMCFARQHNTSDNNNQCLLGANGNILLHLNPQKPGAIDNQPLVTQEPVKATSLTLEGGRLKRTPQLIHGRDYEMVPEPVWRALYHWYGSNLALPRPVIKNSKTDIPELELFPRYLLFLRQQPATRTQQSNIWVNMGMMSLRMFPQHLPRGNVPSPNAPLKRVLAYTGCFSRMQTIKEIHEYLSQRLRIKEEDMRLWLYNSENYLTLLDDEDHRLEYLKIQDEQHLVIEVRNKDMSWPEEMSFIANSSKIDRHKVPTEKGATGLSNLGNTCFMNSSIQCVSNTQPLTQYFISGRHLYELNRTNPIGMKGHMAKCYGDLVQELWSGTQKNVAPLKLRWTIAKYAPRFNGFQQQDSQELLAFLLDGLHEDLNRVHEKPYVELKDSDGRPDWEVAAEAWDNHLRRNRSIVVDLFHGQLRSQVKCKTCGHISVRFDPFNFLSLPLPMDSYMHLEITVIKLDGTTPIRYGLRLNMDEKYTGLKKQLSDLCGLKSEQILFAEVHSSNIKNFPQDNQKVRLSVSGFLCAFEIPIPASPVSACSPIQTDCSSSPSTNGLFTLTTNGDLPRPIFIPNGMPNTVVPCGTEKNVTNGIVNGHMPPLPDDPFTGYIIAVHRKMMRTELYFLSSQKNRPSLFGMPLIVPCTVHTRKKDLYDAVWIQVSRLASPLPPQEASNHAQDCDDSMGYQYPFTLRVVQKDGNSCAWCPWYRFCRGCKIDCGEDRAFIGNACIAVDWDPTALHLRYQTSQERVVEEHESVEQSRRAQAEPINLDSCLRAFTSEEELGENEMYYCSKCKTHCLATKKLDLWRLPPILIIHLKRFQFVNGRWIKSQKIVKFPRESFDPSAFLVPRDPTLCQHKPLTPQGDDFSELRIPAGDVKKVDIQSSAGEEDVLLSKSPSSLSANVTSSPKGSPSSSRKSGASCPSSKNSSPNSSPRTLGRNKGRLRLPQIGSKNKLSNSKENLDTSKENGAGQICELADTLNRRHVLGGSQPELVTPLDHEITLANGFLYEHEACGNGYSNGQLGNHSEEDSTDDQREETHSKPIYNLYAISCHSGILGGGHYVTYAKNPNCKWYCYNDSSCKELHPDEIDTDSAYILFYEQQGIDCAQFLPKTDGKKMADTSSMDEDFESDYKKYCVLQ

Target ORF information:

RefSeq Version	XM_011248990.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin specific peptidase 32 (Usp32), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248990.1

ORF Insert Sequence:

ATGGGCGCCA AGGAGTCACG GATCGGATTC CTCAGCTACG AGGAGGCGCT GAGGAGAGTT 
ACAGATGTTG AGCTGAAACG ACTCAAAGAT GCCTTCAAGA GGACCTGCGG CCTCTCCTAT 
TACATGAGTC AGCACTGCTT CATCAGGGAA GTGCTTGGGG ATGGAGTACC TCCTAAAGTT 
GCTGAGGTGA TTTACTGTTC TTTTGGTGGA ACATCCAAAG GGCTGCATTT CAATAACTTA 
ATAGTTGGAC TCGTCCTCCT TACAAGAGGC AAAGATGAAG AGAAAGCAAA ATATATTTTT 
AGTCTTTTTT CAAGTGAATC CGGGAGCTAT GTTGTACGGG AAGAAATGGA GAGAATGCTC 
CATGTGGTGG ATGGTAAAGT CCCAGATACA CTCAGGAAGT GTTTCTCAGA GGGTGAAAAG 
GTAAACTATG AAAAATTCAG AAATTGGCTT CTTCTAAACA AAGATGCTTT TACCTTCTCT 
CGCTGGCTGT TGTCTGGGGG TGTATATGTG ACCCTCACTG ATGACAGTGA CACTCCTACT 
TTCTACCAAA CTCTGGCTGG AGTCACACAT TTGGAAGAGT CAGACATCAT CGATCTGGAG 
AAACGGTATT GGCTACTGAA GGCCCAATCC CGAACTGGAC GTTTTGACTT AGAGACCTTT 
GGTCCATTGG TTTCACCACC TATTCGCCCT TCTCTGAGTG AAGGATTATT TAATGCTTTT 
GATGAAAATC GTGACAATCA CATAGACTTC AAGGAGATAT CCTGTGGGTT ATCAGCCTGC 
TGCAGGGGCC CCCTGGCTGA AAGACAAAAA TTTTGCTTCA AGGTGTTTGA TGTTGACCGT 
GATGGAGTTC TCTCCAGGGT TGAACTGAAA GACATGGTAG TTGCACTTTT GGAGGTCTGG 
AAAGATAACC GCACTGATGA TATTCCTGAA TTGCATATGG ATTTGTCAGA CATTGTAGAA 
AGGATATTGA ATGCACATGA TACCACAAAG GTGGGCCATC TTACTCTTGA AGACTATCAG 
ATATGGAGTG TGAAAAATGT TCTTGCCAAT GAGTTTTTGA ATCTCCTCTT CCAGGTATGC 
CACATAGTTC TGGGGTTAAG ACCAGCTACT CCAGAAGAAG AAGGACAAAT TATTAGAGGA 
TGGTTGGAAC GAGAAAGCAG GTATGGTCTG CAGCCAGGAC ACAATTGGTT TATCATCTCC 
ATGCAGTGGT GGCAACAGTG GAAAGAATAT GTCAAATATG ATGCCAGCCC TGTTGTAATT 
GAGCCATCAT CTGTTCTAAA TGGAGGAAAA TTTTCATTTG GAACAGCAGC ACATCCTATA 
GAGCATGGAG AAGATAGAAT CAGTAATAAC CTTGGTTATA TGAATACCAC AGAAGAGAAA 
TACTCAGACA ACATTTCTTC TGCATCTGAA GCCTCAGAGT CTACTGGCAG TGGCTTTCTG 
TATTCTGGCA CACCAGGGGC AGATATGTGC TTTGCAAGGC AGCATAACAC TTCTGACAAT 
AACAACCAGT GTTTACTAGG AGCCAATGGG AATATCCTTT TGCACCTTAA TCCTCAGAAG 
CCAGGAGCAA TTGATAACCA GCCACTAGTA ACTCAGGAAC CAGTAAAGGC TACATCATTA 
ACACTAGAAG GAGGGCGATT AAAACGAACT CCACAGCTAA TCCATGGAAG AGATTATGAA 
ATGGTCCCAG AACCCGTGTG GAGAGCACTT TATCATTGGT ATGGATCAAA CCTGGCCCTT 
CCTAGACCAG TAATCAAGAA TAGCAAAACG GACATCCCAG AACTAGAATT ATTTCCTCGC 
TATCTTCTCT TCCTTCGACA GCAGCCTGCC ACACGGACAC AGCAATCTAA CATCTGGGTG 
AATATGGGTA TGATGAGCCT GAGAATGTTT CCTCAGCATT TGCCTAGAGG AAATGTACCC 
TCTCCAAATG CGCCTCTAAA GCGAGTGTTA GCCTATACAG GCTGTTTTAG TAGAATGCAA 
ACCATCAAGG AAATTCATGA ATATCTGTCT CAAAGGCTTC GCATTAAAGA GGAAGATATG 
CGGCTGTGGC TGTACAATAG TGAGAACTAC CTGACTCTTC TGGATGATGA GGATCACAGA 
TTGGAATATT TGAAAATCCA GGATGAGCAG CACCTGGTAA TTGAAGTTCG AAACAAAGAT 
ATGAGTTGGC CTGAAGAAAT GTCTTTTATA GCAAACAGTA GCAAAATAGA TAGACATAAA 
GTTCCCACAG AAAAGGGCGC CACAGGTCTG AGTAACCTGG GAAACACATG CTTCATGAAC 
TCAAGCATCC AGTGTGTCAG CAATACACAA CCACTGACAC AGTACTTTAT CTCAGGGAGG 
CATCTTTATG AACTCAACAG GACAAATCCC ATTGGTATGA AAGGCCATAT GGCTAAATGT 
TATGGCGATT TGGTGCAAGA ACTTTGGAGT GGAACTCAGA AGAATGTTGC CCCCTTAAAG 
CTCCGGTGGA CCATAGCAAA ATATGCTCCC AGGTTTAATG GATTCCAGCA ACAAGACTCC 
CAAGAACTTC TGGCTTTCCT CTTGGATGGT CTTCATGAAG ATCTCAACCG AGTCCACGAG 
AAACCATATG TAGAACTAAA AGACAGTGAC GGCCGACCCG ACTGGGAAGT AGCTGCAGAG 
GCCTGGGACA ACCATCTAAG GAGAAATAGA TCAATTGTTG TGGACTTGTT CCATGGTCAG 
CTAAGATCCC AAGTCAAATG CAAGACATGT GGGCATATAA GTGTCCGATT CGACCCTTTT 
AATTTTTTGT CTTTACCACT ACCAATGGAC AGTTACATGC ATTTAGAAAT AACAGTAATT 
AAATTAGATG GTACTACCCC CATACGATAT GGACTAAGAC TGAATATGGA TGAAAAGTAT 
ACAGGTTTGA AAAAGCAGCT GAGTGATCTC TGTGGACTTA AATCAGAACA AATCCTTTTT 
GCAGAAGTGC ATAGTTCAAA CATAAAGAAC TTCCCTCAGG ACAACCAAAA AGTTCGACTC 
TCAGTGAGTG GATTTTTGTG TGCATTTGAA ATTCCTATTC CTGCATCTCC AGTTTCAGCT 
TGCAGTCCAA TTCAAACAGA TTGCTCTTCT TCTCCATCTA CAAATGGACT GTTTACCTTG 
ACTACCAATG GGGATCTGCC TCGACCTATA TTCATCCCCA ATGGAATGCC AAATACAGTT 
GTACCATGTG GAACTGAGAA GAATGTCACA AATGGAATAG TGAATGGTCA TATGCCGCCT 
CTTCCTGATG ACCCCTTTAC AGGCTACATC ATTGCAGTCC ACCGAAAAAT GATGAGGACA 
GAACTGTATT TCCTGTCATC TCAGAAGAAT CGCCCCAGTC TCTTTGGGAT GCCACTGATT 
GTTCCATGTA CTGTACATAC CCGGAAAAAA GATCTCTATG ATGCAGTTTG GATTCAAGTA 
TCTCGATTAG CCAGTCCGCT CCCACCTCAA GAAGCTAGCA ACCATGCACA GGATTGTGAT 
GACAGCATGG GCTATCAATA TCCATTCACT CTCCGAGTTG TGCAGAAAGA TGGAAACTCC 
TGTGCCTGGT GTCCATGGTA TAGATTTTGC AGAGGCTGTA AAATCGATTG TGGAGAAGAC 
AGAGCTTTCA TTGGAAATGC CTGTATTGCT GTGGATTGGG ACCCTACAGC CCTTCACCTC 
CGCTATCAAA CATCACAGGA AAGAGTTGTA GAGGAGCATG AAAGTGTGGA ACAGAGTCGG 
CGAGCACAAG CTGAGCCCAT CAACCTGGAC AGCTGTCTCC GTGCCTTCAC CAGTGAGGAG 
GAACTGGGGG AAAATGAGAT GTACTACTGC TCCAAATGTA AAACACACTG TTTGGCAACC 
AAGAAACTGG ACCTCTGGAG GCTTCCTCCA ATCCTGATTA TTCACCTTAA ACGATTTCAG 
TTTGTAAATG GACGGTGGAT AAAATCACAG AAAATTGTTA AATTTCCTCG TGAAAGTTTT 
GACCCTAGTG CTTTTTTAGT ACCAAGAGAC CCAACTCTTT GCCAGCATAA ACCACTCACA 
CCCCAGGGGG ATGACTTCTC TGAGCTAAGG ATCCCAGCAG GAGATGTGAA GAAAGTGGAT 
ATCCAGAGCT CAGCAGGGGA GGAGGATGTG CTCCTGAGCA AGAGCCCATC TTCACTCAGT 
GCAAATGTCA CCAGTAGCCC AAAAGGTTCA CCTTCTTCAT CAAGAAAAAG TGGAGCCAGC 
TGTCCCTCCA GCAAAAATAG CAGCCCTAAC AGCAGCCCAC GGACCTTGGG GAGGAACAAA 
GGGAGGCTCC GGCTGCCTCA AATTGGCAGC AAAAATAAAT TGTCCAATAG TAAGGAGAAT 
TTGGATACCA GCAAAGAGAA TGGGGCTGGG CAGATCTGTG AACTGGCTGA CACCTTGAAC 
CGAAGACATG TATTGGGAGG CAGCCAGCCT GAGCTGGTCA CTCCTCTGGA TCATGAGATC 
ACTTTGGCTA ATGGATTTCT TTATGAGCAT GAGGCATGTG GCAATGGCTA TAGCAATGGT 
CAGCTTGGAA ACCATAGTGA AGAAGACAGC ACTGATGACC AAAGAGAAGA AACACACAGT 
AAACCTATTT ATAATCTATA TGCAATTTCG TGCCATTCAG GAATTCTGGG TGGGGGCCAT 
TACGTCACTT ATGCCAAAAA CCCAAACTGC AAGTGGTACT GTTACAATGA CAGCAGCTGT 
AAGGAGCTTC ACCCTGATGA GATAGACACC GACTCTGCCT ACATTCTTTT CTATGAGCAG 
CAGGGGATAG ACTGTGCACA GTTTCTGCCA AAGACAGACG GCAAGAAGAT GGCGGACACG 
AGCAGCATGG ACGAAGACTT TGAGTCTGAC TACAAGAAGT ACTGTGTGCT ACAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene-trap mutagenesis using Mol/MSM-1 embryonic stem cells from MSM/Ms mice.
Mammalian genome : official journal of the International Mammalian Genome Society24(5-6)228-39(2013 Jun)
Nakahara M,Tateyama H,Araki M,Nakagata N,Yamamura K,Araki K

USP32 is an active, membrane-bound ubiquitin protease overexpressed in breast cancers.
Mammalian genome : official journal of the International Mammalian Genome Society21(7-8)388-97(2010 Aug)
Akhavantabasi S,Akman HB,Sapmaz A,Keller J,Petty EM,Erson AE

Characterization of an exchangeable gene trap using pU-17 carrying a stop codon-beta geo cassette.
Development, growth & differentiation47(3)163-72(2005 Apr)
Taniwaki T,Haruna K,Nakamura H,Sekimoto T,Oike Y,Imaizumi T,Saito F,Muta M,Soejima Y,Utoh A,Nakagata N,Araki M,Yamamura K,Araki K

Identification and developmental analysis of genes expressed by dopaminergic neurons of the substantia nigra pars compacta.
Molecular and cellular neurosciences25(3)394-405(2004 Mar)
Thuret S,Bhatt L,O'Leary DD,Simon HH

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Nucleic acids research31(1)278-81(2003 Jan)
Stryke D,Kawamoto M,Huang CC,Johns SJ,King LA,Harper CA,Meng EC,Lee RE,Yee A,L'Italien L,Chuang PT,Young SG,Skarnes WC,Babbitt PC,Ferrin TE

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Usp32 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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