otofa cDNA ORF clone, Danio rerio(zebrafish)

The following otofa gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the otofa cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa00833 NM_001030112.2
Latest version!
Danio rerio otoferlin a (otofa), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
ODa42604 XM_009294732.3
Latest version!
Danio rerio otoferlin a (otofa), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
ODa42605 XM_009294733.3
Latest version!
Danio rerio otoferlin a (otofa), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
ODa42606 XM_009294734.3
Latest version!
Danio rerio otoferlin a (otofa), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
ODa42607 XM_009294736.3
Latest version!
Danio rerio otoferlin a (otofa), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
ODa42607 XM_009294735.2
Latest version!
Danio rerio otoferlin a (otofa), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
ODa42608 XM_021468441.1
Latest version!
Danio rerio otoferlin a (otofa), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
ODa42609 XM_009294738.3
Latest version!
Danio rerio otoferlin a (otofa), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
ODa42610 XM_009294739.3
Latest version!
Danio rerio otoferlin a (otofa), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
ODa42611 XM_009294740.3
Latest version!
Danio rerio otoferlin a (otofa), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
ODa42612 XM_021468442.1
Latest version!
Danio rerio otoferlin a (otofa), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
ODa42613 XM_009294742.3
Latest version!
Danio rerio otoferlin a (otofa), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
ODa42614 XM_009294743.2
Latest version!
Danio rerio otoferlin a (otofa), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
ODa42614 XM_021468443.1
Latest version!
Danio rerio otoferlin a (otofa), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
ODa42615 XM_009294744.3
Latest version!
Danio rerio otoferlin a (otofa), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
View Old Accession Versions >>
ODa42615 XM_009294744.2 Danio rerio otoferlin a (otofa), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
ODa42605 XM_009294733.2 Danio rerio otoferlin a (otofa), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
ODa42604 XM_009294732.2 Danio rerio otoferlin a (otofa), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
ODa42606 XM_009294734.2 Danio rerio otoferlin a (otofa), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
ODa42607 XM_009294736.2 Danio rerio otoferlin a (otofa), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
ODa42611 XM_009294740.2 Danio rerio otoferlin a (otofa), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
ODa42613 XM_009294742.2 Danio rerio otoferlin a (otofa), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
ODa42609 XM_009294738.2 Danio rerio otoferlin a (otofa), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
ODa42610 XM_009294739.2 Danio rerio otoferlin a (otofa), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa00833
    Clone ID Related Accession (Same CDS sequence) NM_001030112.2
    Accession Version NM_001030112.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5979bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-27
    Organism Danio rerio(zebrafish)
    Product otoferlin
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AL929014.28 and BX000361.8. On May 12, 2008 this sequence version replaced NM_001030112.1. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN01765705 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGCTTTAG TGGTGCATCT GAAGACTGTC ACGGAGCTCC GTGGGAAGGG GGACCGAATC 
    GCTAAAGTCA CTTTCAGAGG TCTCTCCTTC TTCTCTCGGG TGTTGGAAAA CTGCGAGGAT
    GAAGCTCGAT TTGAGCAGGC GTTCCGATGG CCAATTGGGA GTCAAGTGGA TGGCGACGAG
    ATGCTGGAGA TCCAAGTGTT TAATTACAGC AAAGTCTTCA CTAATAGGTT AATCGGCACA
    TTCCGCATGG TTCTTCAGAA GGTGGTGGAG GAGGGACACC TGGAGGTGTC TGACACACTC
    ATTGACGACA ATAACACAGC CATACAGACA ACCATTTCCA TAGAGATCAA ATATCAGACC
    ATGGACGGGA GTGTAAAGGT GTGGAGCGAT GGTGAATTCC TTGATATTCC AGATGACCTT
    GATGGAACCT TCCAGTTTGA GACAGATAGT CTTCTCTCAG GACGCAGCCA GAGTTCTGGA
    ACTTCACCTG GACGATCCAT TCATGGCATT CCCACATTCC GCAAGGCAGG AAAAGGAGTG
    TTCTCAGCCA TGAAGCTGGG CAAGACCCGC ACCTCTAAAG ATGACCACAA AAAAGGAGAT
    GATGCTGCCA TTCTGGATGC AGAAGATCTG GATCGAAAAG CCATGCGTCT AGGCGGCATT
    CTTGACCCTG ATACAATCTC ACTGGCCTCG GTTACTGCAG TGACCACTAA TGTCTCCAAC
    AAAAGATCAA AGCCTGACAT TAAAATGGAG CCGAGTTCAG GGCGTCCGGT TGATTATCAG
    ATCAGTGTTA CAGTAATCGA GGCCCGTCAG CTGGTCGGAT TGAACATGGA CCCTGTGGTT
    TGCGTGGAAA TTGGAGAGGA GAAGAAGTAC ACGTCAATGA AGGAGTCAAC AAACTGCCCT
    TACTACAATG AGTATTTTGT CTTCGACTTC CACGTGCCTC CAGACGTCAT GTTTGACAAA
    ATTATAAAGA TTTCAGTAAT ACATTCTAAA AACCTTCTGC GAAGCGGCAC CCTCGTGGGC
    ACCTTCAAAC TAGACGTGGG GACTGTTTAC ACACAACCTG AACATCAGTT TCATCACAAA
    TGGGCGATGC TGTCAGACCC TGATGACATC ACGACTGGCT GCAAAGGTTA TGTTAAATGT
    GACATCGCAG TCGTGGGAAA AGGAGACAAC ATCAAGACTC CACACAAGGC CAGTGAAGCA
    GACGAGGATG ACATAGAGGG GAATCTTCTC TTGCCGGAGG GTGTTCCATC AGAGAGACAG
    TGGGCTCGGT TTTATGTTAA GATTTATAGA GCTGAGGGTC TGCCTAAAAT GAACACCAGT
    ATCATGGCCA ACGTGAAGAA AGCCTTTATT GGGGAAAACA GGGACCTTGT GGACCCTTAC
    GTCCTGGTGC AATTTGCAGG GCAAAAGGGC AAAACATCTG TTCAGAAGAG CAGCTACGAG
    CCCATCTGGA ACGAGCAAGT CATCTTCACG GAGATGTTCC CACCTTTGTG TAGGCGACTG
    AAAGTTCAGA TCCGTGATTC AGACAAGGTG AACGATGTTG CCATAGGAAC CCACTTCATT
    GATCTACGAA AGGTTTCCAA TGATGGAGAC AAAGGTTTCT TGCCCACTAT GGGTCCGGCT
    TGGGTGAATA TGTATGGGTC GACCCGTAAC TACACCCTGA TGGACGAACA CCAGGACCTG
    AATGAGGGGT TGGGGGAAGG GGTGTCCTTC AGAGCACGTC TGCTCATTAG TATCGCGGTG
    GAGATTCTAG ACACCACCTC TGCTGAGATC ATGAGCTCCA CTGAAGTACA CATGGAGCCG
    GTGTCCAACA TCTCAGAGAG TGCTACAGGA AAGATGGAAG AATTTTTCCT TTTCGGGTCA
    TTCTTGGAGG CCACAATGAT AGACAGAAAA ATAGGAGATA AACCCATCAG CTTTGAAGTC
    ACCATCGGTA ACTATGGTAA CCAGATTGAT GGTGTGAGCA AGCCTGCATT AGCGAAGAAG
    AAGAAGGAGG GTGGAGGAGA GAGTGAAGAA GAGGAGTCTG AGCTTATCCA CAACTCCAGC
    GAGGAAGAGG CAGAGGATGA TGGAGATCTG ACCTCTGTAC CGTCTACACC TCCTATGAAG
    CCTGTTATCA CCGACAGAAA TTACTTCCAC TTGCCTTACT TTGAAAAGAA ACCCTGCATT
    TACATCAAGA GCTGGTGGCA GGACCAGCGG AGACGTCTCT ACAACTCCAA CATAATGGAC
    AAGATAGCTG ATAAACTGGA GGAAGGTCTG AATGATGTTC AAGAGATCAT AAAAACAGAG
    AAGGCATATC CAGAACGAAG ACTAAGAGGT GTATTAGAAG AACTTAGCAC AAGCTGCAGC
    CGGTTTGTTA CACTTGCAAA CAAGGATCAG AATCTTTCTG GAAGAACCAA GCTGGACAGA
    GAGAGGCTGA AGTCCTGTAT GAGAGAAATG GAGAGTATGG GTCAGCAGGC GAAGACCATC
    CGCACACAAG TGAAAAGAAA CACAGTTCGA GATAAACTCA AGCTAGTGTT GAATTTCCTT
    CAGAGGCTTC GATTCTTAGC AGACGAGCCC CAGCACAGCA TCCCTGATGT GTTCATATGG
    ATGATTAGCA ACAACAAACG CATCGCATAC GCCCGCATAC CCTCCAAAGA CATCCTCTAC
    TCAATTGTCG ATGAAGAGAT GGGCAAAGAC TGTGGAAAAG TTAAAGCTGT TTTTCTCAGG
    CTGCCTGGAA AGAAAGGTTT TGGGCCTGCT GGCTGGACAG TTCAGGCTAA ATTAGAGATG
    TATTTGTGGC TGGGCCTGAA TAAACAGAGG AAAGACTTCC TGATTGGCCT CCCTAGTGGC
    TTTGAAGAAA ACAAGGCAGT AAAGGGAATT GGCATACAAG CTGTCCCTCC CATCAGCCTA
    GTTTATAACA TGAAGCAGGT GTTTCAGCTG AGGGCCCACA TGTATCAAGC TCGCAGTCTG
    TTTGCTGCGG ACACTAGTGG CCTGTCTGAT CCATTTGCAA GGGTTTTCTT CTCCACCCAC
    AGCCAGGTCA CGGAGGTCCT CAGTGAGACT CTCTGTCCAA CATGGGATCA GCTGCTTGTG
    TTTGATAATG TTGAACTTTA CGGTGAGGCC GGTGAACTCC GTGATGACCC TCCAATCATT
    GTCATTGAGT TGTATGATCA AGACACTGTG GGGAAAGCTG AGTTTATTGG CCGAACATTT
    GCAAAACCAC TTACTAAGAT GGTTGATGAA CACTACGGCC CACCGCGGTT TCCTCCCCAG
    CTCGAGTATT ATCAGATCTA CCGAGGAAAC TGTGCTGCGG GAGATCTATT GGCTGCTTTT
    GAGCTTCTAC AGATTGGTCC AGCAGGGCGG GCAGCACTTC CTCCAATCGA TGGACCGACC
    GATTCTGACC GTGGGCCCAT TCTGCCTGTT CCATTGGGAA TTCGACCAGT TCTCAGCAGA
    TATCGCATCG AGGTCCTGTT TTGGGGTCTG AGGGACCTTA AAAGGGTCAA CCTGGCTCAG
    GTGGACCGAC CTCGTGTGGA CATTGAGTGT GCAGGGAAAG GAGTTCAGTC AGCCCTAATT
    CAAAACTACA AGAAGAATCC AAACTTCAGC ACATTAGTAA AGTGGTTTGA AGTGGATCTG
    CCAGAAAATG AGCTGCTCCA TCCACCACTT AATATTCGGG TAGTGGATTG CAGGGCATTC
    GGACGCTACA TTTTGGTGGG GTCTCATGCT GTGACCACCC TTCGCAAGTT CATCTACAGC
    CCCCCAGACA AGACTGCTAA CAACTGGGCT CACACAGGTG ACATTGTGGT CAACATGAGC
    CCTGAACCTA ACATTAAGAA GATGGACACG GTTGTCAAGA TTGAAGCTAC CACCGATGCG
    GTTGTTAAAG TTGACTTGAA TGAGGATGAG AAGGAGAAAG AAAAGAAGAA GAAGAAGAAG
    AAGAAAGGAG AAGAAGTGGA GGAAGAGGAG CCGGATGAGA GCATGCTGGA CTGGTGGTCC
    AAATACTTTG CCTCAATAGA GACTATGATG GAGAATCTAA GAGCTCAGGA GGCCGCTCAG
    GCAGAGGCAG AGGAAAGAGA AGATTTGGAG ATTGCAGCAG AGAGTGCAGA GATCAAAGCT
    GATGACTTTC CTATGAAAGG CACCAAACCT AAAGAGAAGA GCAAAGACAA GAAGAGCACC
    AAGGACAAAA AGAAGAACAA CGATGGCACA GAAAAACGAC CTCCAAAACC AAAAGTTGAT
    GAACTCATGG TGTACAATAA AGAGCTGGAG AGTGAGTTCG GTAGCTTTGA GGACTGGCTT
    CACACCTTCA ACCTGTACCG AGGAAAGGCT GGAGATGATG ATGACCACAA TGTGGTTGAT
    GAGGACAGAA TTGTGGGCAG ATTCAAGGGC TCCCTCTGTA TGTATAAACT ACCACTGTCT
    GAGGAGATCA CCAGAGAGGC AGGATTTGAT CCAAACATGG GCATGTTCCA GAGTATTCCT
    CACAATGACC CTATCAACGT TCTTGTCAGG ATTTATATCA TTCGAGCCAC AGACCTGCAT
    CCTGCAGATA TAAATGGAAA GGCCGACCCT TATATAGTCA TTAAATTGGG AAAGTCAGAC
    ATTCGAGACA AGGAGAACTA CATATCCAAG CAGCTGAATC CTGTCTTTGG AAAGTCATTT
    GATATAGAGG CCACGTTCCC AATGGAGTCT ATGCTGACTG TGGCAGTGTA TGACTGGGAT
    TTAGTCGGAA CTGATGACCT GATTGGTGAG ACTAAGATTG ATTTGGAGAA CAGATACTAC
    AGCAAACACA GAGCCACATG TGGCATTGCT TCCAACTACT CTGTCCATGG TTATAATGTA
    TGGAGAGATC CTCAGAAACC AGCTCAGATC CTGGCTAAGT TGTGCAAAGA AGGTAAACTG
    GATGGACCTC ACTATGGACC TGGGGGAAGA GTCAAAGTTG CAAACCGGAT CTTTCTTGGG
    CCGACAGAAA TTGAGGATGA GAGTGGTCTG AAAAAACAAA CAGAAGAACA CTTGGCGCTC
    ACAGTCCTTA GACATTGGGA GGAAATCCCA CGTGTTGGTT GCAAACTCAT CCCTGAGCAT
    GTGGAGACGA GGCCTCTCCT CAACCCAGAC AAGCCAGGCA TAGAGCAGGG AAGGATTGAA
    ATGTGGGTGG ACATGTTTCC TATGGATGTC CCAGCACCAG GACCAGCCAT TGATATATCG
    CCACGCAAAC CAAAGAGATA TGAGCTCAGG GTGATAATAT GGAATACTGA CGAGGTAATT
    CTGGAAGACG ATGATTACTT CACAGGGGAA AAGTCCAGTG ACATTTTTGT GAGGGGCTGG
    CTTAAAGGAC AGCAGGAGGA CAAACAGGAC ACCGATGTAC ATTATCACTC TCTAACTGGA
    GAGGGAAACT TCAACTGGCG CTTTGTCTTC CCCTTTGACT ATCTCATGGC CGAGGAGAAG
    ATTGTTATCT CCAAGAAGGA ATCAATGTTT TCCTGGGATG AGACTGAGTA TAAGATCCCT
    GCTCGACTCA CCCTTCAAGT ATGGGATGCC GATCACTTTT CTGCAGATGA TTTTCTGGGT
    GCGATTGAGT TGGACTTGAA TAAGTTTCCG CGTGGGGCAA AAACAGCCAA GCAGTGCTCT
    CTAGACATGG TTCTCAAAGA GCATGAGCTG CCAACCATCT CCATCTTCAA ACAAAAGAGA
    GTCAAGGGCT GGTGGCCATT TGTGGCCCAA AATGAGAATG ATGAATTTGA GCTAACGGGA
    AAAGTAGAGG CAGAGCTCCA TCTGTTGACA GCAGAGGAGG CAGAGAAGAG TCCAGTCGGC
    CTGGGGAGGA ATGATCCGGA GCCACTTGAG AAACCAAATC GTCCAGACAC CAGTCTAATG
    TGGTTCATGA ACCCTCTGAG GTCCATACGA TACTTCATTT GGCACAACTA CCGCTGGCTG
    ATCCTGAAGG CTCTGGCTCT GCTGCTGCTC CTTCTCCTCG TTGGGCTCTT CCTCTACTCT
    ATTCCTGGAT ACCTGGTCAA GAAACTCCTA GGGGCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001025283.1
    CDS1..5979
    Translation

    Target ORF information:

    RefSeq Version NM_001030112.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio otoferlin a (otofa), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001030112.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGCTTTAG TGGTGCATCT GAAGACTGTC ACGGAGCTCC GTGGGAAGGG GGACCGAATC 
    GCTAAAGTCA CTTTCAGAGG TCTCTCCTTC TTCTCTCGGG TGTTGGAAAA CTGCGAGGAT
    GAAGCTCGAT TTGAGCAGGC GTTCCGATGG CCAATTGGGA GTCAAGTGGA TGGCGACGAG
    ATGCTGGAGA TCCAAGTGTT TAATTACAGC AAAGTCTTCA CTAATAGGTT AATCGGCACA
    TTCCGCATGG TTCTTCAGAA GGTGGTGGAG GAGGGACACC TGGAGGTGTC TGACACACTC
    ATTGACGACA ATAACACAGC CATACAGACA ACCATTTCCA TAGAGATCAA ATATCAGACC
    ATGGACGGGA GTGTAAAGGT GTGGAGCGAT GGTGAATTCC TTGATATTCC AGATGACCTT
    GATGGAACCT TCCAGTTTGA GACAGATAGT CTTCTCTCAG GACGCAGCCA GAGTTCTGGA
    ACTTCACCTG GACGATCCAT TCATGGCATT CCCACATTCC GCAAGGCAGG AAAAGGAGTG
    TTCTCAGCCA TGAAGCTGGG CAAGACCCGC ACCTCTAAAG ATGACCACAA AAAAGGAGAT
    GATGCTGCCA TTCTGGATGC AGAAGATCTG GATCGAAAAG CCATGCGTCT AGGCGGCATT
    CTTGACCCTG ATACAATCTC ACTGGCCTCG GTTACTGCAG TGACCACTAA TGTCTCCAAC
    AAAAGATCAA AGCCTGACAT TAAAATGGAG CCGAGTTCAG GGCGTCCGGT TGATTATCAG
    ATCAGTGTTA CAGTAATCGA GGCCCGTCAG CTGGTCGGAT TGAACATGGA CCCTGTGGTT
    TGCGTGGAAA TTGGAGAGGA GAAGAAGTAC ACGTCAATGA AGGAGTCAAC AAACTGCCCT
    TACTACAATG AGTATTTTGT CTTCGACTTC CACGTGCCTC CAGACGTCAT GTTTGACAAA
    ATTATAAAGA TTTCAGTAAT ACATTCTAAA AACCTTCTGC GAAGCGGCAC CCTCGTGGGC
    ACCTTCAAAC TAGACGTGGG GACTGTTTAC ACACAACCTG AACATCAGTT TCATCACAAA
    TGGGCGATGC TGTCAGACCC TGATGACATC ACGACTGGCT GCAAAGGTTA TGTTAAATGT
    GACATCGCAG TCGTGGGAAA AGGAGACAAC ATCAAGACTC CACACAAGGC CAGTGAAGCA
    GACGAGGATG ACATAGAGGG GAATCTTCTC TTGCCGGAGG GTGTTCCATC AGAGAGACAG
    TGGGCTCGGT TTTATGTTAA GATTTATAGA GCTGAGGGTC TGCCTAAAAT GAACACCAGT
    ATCATGGCCA ACGTGAAGAA AGCCTTTATT GGGGAAAACA GGGACCTTGT GGACCCTTAC
    GTCCTGGTGC AATTTGCAGG GCAAAAGGGC AAAACATCTG TTCAGAAGAG CAGCTACGAG
    CCCATCTGGA ACGAGCAAGT CATCTTCACG GAGATGTTCC CACCTTTGTG TAGGCGACTG
    AAAGTTCAGA TCCGTGATTC AGACAAGGTG AACGATGTTG CCATAGGAAC CCACTTCATT
    GATCTACGAA AGGTTTCCAA TGATGGAGAC AAAGGTTTCT TGCCCACTAT GGGTCCGGCT
    TGGGTGAATA TGTATGGGTC GACCCGTAAC TACACCCTGA TGGACGAACA CCAGGACCTG
    AATGAGGGGT TGGGGGAAGG GGTGTCCTTC AGAGCACGTC TGCTCATTAG TATCGCGGTG
    GAGATTCTAG ACACCACCTC TGCTGAGATC ATGAGCTCCA CTGAAGTACA CATGGAGCCG
    GTGTCCAACA TCTCAGAGAG TGCTACAGGA AAGATGGAAG AATTTTTCCT TTTCGGGTCA
    TTCTTGGAGG CCACAATGAT AGACAGAAAA ATAGGAGATA AACCCATCAG CTTTGAAGTC
    ACCATCGGTA ACTATGGTAA CCAGATTGAT GGTGTGAGCA AGCCTGCATT AGCGAAGAAG
    AAGAAGGAGG GTGGAGGAGA GAGTGAAGAA GAGGAGTCTG AGCTTATCCA CAACTCCAGC
    GAGGAAGAGG CAGAGGATGA TGGAGATCTG ACCTCTGTAC CGTCTACACC TCCTATGAAG
    CCTGTTATCA CCGACAGAAA TTACTTCCAC TTGCCTTACT TTGAAAAGAA ACCCTGCATT
    TACATCAAGA GCTGGTGGCA GGACCAGCGG AGACGTCTCT ACAACTCCAA CATAATGGAC
    AAGATAGCTG ATAAACTGGA GGAAGGTCTG AATGATGTTC AAGAGATCAT AAAAACAGAG
    AAGGCATATC CAGAACGAAG ACTAAGAGGT GTATTAGAAG AACTTAGCAC AAGCTGCAGC
    CGGTTTGTTA CACTTGCAAA CAAGGATCAG AATCTTTCTG GAAGAACCAA GCTGGACAGA
    GAGAGGCTGA AGTCCTGTAT GAGAGAAATG GAGAGTATGG GTCAGCAGGC GAAGACCATC
    CGCACACAAG TGAAAAGAAA CACAGTTCGA GATAAACTCA AGCTAGTGTT GAATTTCCTT
    CAGAGGCTTC GATTCTTAGC AGACGAGCCC CAGCACAGCA TCCCTGATGT GTTCATATGG
    ATGATTAGCA ACAACAAACG CATCGCATAC GCCCGCATAC CCTCCAAAGA CATCCTCTAC
    TCAATTGTCG ATGAAGAGAT GGGCAAAGAC TGTGGAAAAG TTAAAGCTGT TTTTCTCAGG
    CTGCCTGGAA AGAAAGGTTT TGGGCCTGCT GGCTGGACAG TTCAGGCTAA ATTAGAGATG
    TATTTGTGGC TGGGCCTGAA TAAACAGAGG AAAGACTTCC TGATTGGCCT CCCTAGTGGC
    TTTGAAGAAA ACAAGGCAGT AAAGGGAATT GGCATACAAG CTGTCCCTCC CATCAGCCTA
    GTTTATAACA TGAAGCAGGT GTTTCAGCTG AGGGCCCACA TGTATCAAGC TCGCAGTCTG
    TTTGCTGCGG ACACTAGTGG CCTGTCTGAT CCATTTGCAA GGGTTTTCTT CTCCACCCAC
    AGCCAGGTCA CGGAGGTCCT CAGTGAGACT CTCTGTCCAA CATGGGATCA GCTGCTTGTG
    TTTGATAATG TTGAACTTTA CGGTGAGGCC GGTGAACTCC GTGATGACCC TCCAATCATT
    GTCATTGAGT TGTATGATCA AGACACTGTG GGGAAAGCTG AGTTTATTGG CCGAACATTT
    GCAAAACCAC TTACTAAGAT GGTTGATGAA CACTACGGCC CACCGCGGTT TCCTCCCCAG
    CTCGAGTATT ATCAGATCTA CCGAGGAAAC TGTGCTGCGG GAGATCTATT GGCTGCTTTT
    GAGCTTCTAC AGATTGGTCC AGCAGGGCGG GCAGCACTTC CTCCAATCGA TGGACCGACC
    GATTCTGACC GTGGGCCCAT TCTGCCTGTT CCATTGGGAA TTCGACCAGT TCTCAGCAGA
    TATCGCATCG AGGTCCTGTT TTGGGGTCTG AGGGACCTTA AAAGGGTCAA CCTGGCTCAG
    GTGGACCGAC CTCGTGTGGA CATTGAGTGT GCAGGGAAAG GAGTTCAGTC AGCCCTAATT
    CAAAACTACA AGAAGAATCC AAACTTCAGC ACATTAGTAA AGTGGTTTGA AGTGGATCTG
    CCAGAAAATG AGCTGCTCCA TCCACCACTT AATATTCGGG TAGTGGATTG CAGGGCATTC
    GGACGCTACA TTTTGGTGGG GTCTCATGCT GTGACCACCC TTCGCAAGTT CATCTACAGC
    CCCCCAGACA AGACTGCTAA CAACTGGGCT CACACAGGTG ACATTGTGGT CAACATGAGC
    CCTGAACCTA ACATTAAGAA GATGGACACG GTTGTCAAGA TTGAAGCTAC CACCGATGCG
    GTTGTTAAAG TTGACTTGAA TGAGGATGAG AAGGAGAAAG AAAAGAAGAA GAAGAAGAAG
    AAGAAAGGAG AAGAAGTGGA GGAAGAGGAG CCGGATGAGA GCATGCTGGA CTGGTGGTCC
    AAATACTTTG CCTCAATAGA GACTATGATG GAGAATCTAA GAGCTCAGGA GGCCGCTCAG
    GCAGAGGCAG AGGAAAGAGA AGATTTGGAG ATTGCAGCAG AGAGTGCAGA GATCAAAGCT
    GATGACTTTC CTATGAAAGG CACCAAACCT AAAGAGAAGA GCAAAGACAA GAAGAGCACC
    AAGGACAAAA AGAAGAACAA CGATGGCACA GAAAAACGAC CTCCAAAACC AAAAGTTGAT
    GAACTCATGG TGTACAATAA AGAGCTGGAG AGTGAGTTCG GTAGCTTTGA GGACTGGCTT
    CACACCTTCA ACCTGTACCG AGGAAAGGCT GGAGATGATG ATGACCACAA TGTGGTTGAT
    GAGGACAGAA TTGTGGGCAG ATTCAAGGGC TCCCTCTGTA TGTATAAACT ACCACTGTCT
    GAGGAGATCA CCAGAGAGGC AGGATTTGAT CCAAACATGG GCATGTTCCA GAGTATTCCT
    CACAATGACC CTATCAACGT TCTTGTCAGG ATTTATATCA TTCGAGCCAC AGACCTGCAT
    CCTGCAGATA TAAATGGAAA GGCCGACCCT TATATAGTCA TTAAATTGGG AAAGTCAGAC
    ATTCGAGACA AGGAGAACTA CATATCCAAG CAGCTGAATC CTGTCTTTGG AAAGTCATTT
    GATATAGAGG CCACGTTCCC AATGGAGTCT ATGCTGACTG TGGCAGTGTA TGACTGGGAT
    TTAGTCGGAA CTGATGACCT GATTGGTGAG ACTAAGATTG ATTTGGAGAA CAGATACTAC
    AGCAAACACA GAGCCACATG TGGCATTGCT TCCAACTACT CTGTCCATGG TTATAATGTA
    TGGAGAGATC CTCAGAAACC AGCTCAGATC CTGGCTAAGT TGTGCAAAGA AGGTAAACTG
    GATGGACCTC ACTATGGACC TGGGGGAAGA GTCAAAGTTG CAAACCGGAT CTTTCTTGGG
    CCGACAGAAA TTGAGGATGA GAGTGGTCTG AAAAAACAAA CAGAAGAACA CTTGGCGCTC
    ACAGTCCTTA GACATTGGGA GGAAATCCCA CGTGTTGGTT GCAAACTCAT CCCTGAGCAT
    GTGGAGACGA GGCCTCTCCT CAACCCAGAC AAGCCAGGCA TAGAGCAGGG AAGGATTGAA
    ATGTGGGTGG ACATGTTTCC TATGGATGTC CCAGCACCAG GACCAGCCAT TGATATATCG
    CCACGCAAAC CAAAGAGATA TGAGCTCAGG GTGATAATAT GGAATACTGA CGAGGTAATT
    CTGGAAGACG ATGATTACTT CACAGGGGAA AAGTCCAGTG ACATTTTTGT GAGGGGCTGG
    CTTAAAGGAC AGCAGGAGGA CAAACAGGAC ACCGATGTAC ATTATCACTC TCTAACTGGA
    GAGGGAAACT TCAACTGGCG CTTTGTCTTC CCCTTTGACT ATCTCATGGC CGAGGAGAAG
    ATTGTTATCT CCAAGAAGGA ATCAATGTTT TCCTGGGATG AGACTGAGTA TAAGATCCCT
    GCTCGACTCA CCCTTCAAGT ATGGGATGCC GATCACTTTT CTGCAGATGA TTTTCTGGGT
    GCGATTGAGT TGGACTTGAA TAAGTTTCCG CGTGGGGCAA AAACAGCCAA GCAGTGCTCT
    CTAGACATGG TTCTCAAAGA GCATGAGCTG CCAACCATCT CCATCTTCAA ACAAAAGAGA
    GTCAAGGGCT GGTGGCCATT TGTGGCCCAA AATGAGAATG ATGAATTTGA GCTAACGGGA
    AAAGTAGAGG CAGAGCTCCA TCTGTTGACA GCAGAGGAGG CAGAGAAGAG TCCAGTCGGC
    CTGGGGAGGA ATGATCCGGA GCCACTTGAG AAACCAAATC GTCCAGACAC CAGTCTAATG
    TGGTTCATGA ACCCTCTGAG GTCCATACGA TACTTCATTT GGCACAACTA CCGCTGGCTG
    ATCCTGAAGG CTCTGGCTCT GCTGCTGCTC CTTCTCCTCG TTGGGCTCTT CCTCTACTCT
    ATTCCTGGAT ACCTGGTCAA GAAACTCCTA GGGGCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42604
    Clone ID Related Accession (Same CDS sequence) XM_009294732.2 , XM_009294732.3
    Accession Version XM_009294732.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6141bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product otoferlin isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009294732.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCTTTAG TGGTGCATCT GAAGACTGTC ACGGAGCTCC GTGGGAAGGG GGACCGAATC 
    GCTAAAGTCA CTTTCAGAGG TCTCTCCTTC TTCTCTCGGG TGTTGGAAAA CTGCGAGGAT
    GAAGCTCGAT TTGAGCAGGC GTTCCGATGG CCAATTGGGA GTCAAGTGGA TGGCGACGAG
    ATGCTGGAGA TCCAAGTGTT TAATTACAGC AAAGTCTTCA CTAATAGGTT AATCGGCACA
    TTCCGCATGG TTCTTCAGAA GGTGGTGGAG GAGGGACACC TGGAGGTGTC TGACACACTC
    ATTGACGACA ATAACACAGC CATACAGACA ACCATTTCCA TAGAGATCAA ATATCAGACC
    ATGGACGGGA GTGTAAAGGT GTGGAGCGAT GGTGAATTCC TTGATATTCC AGATGACCTT
    GATGGAACCT TCCAGTTTGA GACAGATAGT CTTCTCTCAG GACGCAGCCA GAGTTCTGGA
    ACTTCACCTG GACGATCCAT TCATGGCATT CCCACATTCC GCAAGGCAGG AAAAGGAGTG
    TTCTCAGCCA TGAAGCTGGG CAAGACCCGC ACCTCTAAAG ATGACCACAA AAAAGGAGAT
    GATGCTGCCA TTCTGGATGC AGAAGATCTG GATCGAAAAG CCATGCGTCT AGGCGGCATT
    CTTGACCCTG ATACAATCTC ACTGGCCTCG GTTACTGCAG TGACCACTAA TGTCTCCAAC
    AAAAGATCAA AGCCTGACAT TAAAATGGAG CCGAGTTCAG GGCGTCCGGT TGATTATCAG
    ATCAGTGTTA CAGTAATCGA GGCCCGTCAG CTGGTCGGAT TGAACATGGA CCCTGTGGTT
    TGCGTGGAAA TTGGAGAGGA GAAGAAGTAC ACGTCAATGA AGGAGTCAAC AAACTGCCCT
    TACTACAATG AGTATTTTGT CTTCGACTTC CACGTGCCTC CAGACGTCAT GTTTGACAAA
    ATTATAAAGA TTTCAGTAAT ACATTCTAAA AACCTTCTGC GAAGCGGCAC CCTCGTGGGC
    ACCTTCAAAC TAGACGTGGG GACTGTTTAC ACACAACCTG AACATCAGTT TCATCACAAA
    TGGGCGATGC TGTCAGACCC TGATGACATC ACGACTGGCT GCAAAGGTTA TGTTAAATGT
    GACATCGCAG TCGTGGGAAA AGGAGACAAC ATCAAGACTC CACACAAGGC CAGTGAAGCA
    GACGAGGATG ACATAGAGGG GAATCTTCTC TTGCCGGAGG GTGTTCCATC AGAGAGACAG
    TGGGCTCGGT TTTATGTTAA GATTTATAGA GCTGAGGGTC TGCCTAAAAT GAACACCAGT
    ATCATGGCCA ACGTGAAGAA AGCCTTTATT GGGGAAAACA GGGACCTTGT GGACCCTTAC
    GTCCTGGTGC AATTTGCAGG GCAAAAGGGC AAAACATCTG TTCAGAAGAG CAGCTACGAG
    CCCATCTGGA ACGAGCAAGT CATCTTCACG GAGATGTTCC CACCTTTGTG TAGGCGACTG
    AAAGTTCAGA TCCGTGATTC AGACAAGGTG AACGATGTTG CCATAGGAAC CCACTTCATT
    GATCTACGAA AGGTTTCCAA TGATGGAGAC AAAGGTTTCT TGCCCACTAT GGGTCCGGCT
    TGGGTGAATA TGTATGGGTC GACCCGTAAC TACACCCTGA TGGACGAACA CCAGGACCTG
    AATGAGGGGT TGGGGGAAGG GGTGTCCTTC AGAGCACGTC TGCTCATTAG TATCGCGGTG
    GAGATTCTAG ACACCACCTC TGCTGAGATC ATGAGCTCCA CTGAAGTACA CATGGAGCCG
    GTGTCCAACA TCTCAGAGAG TGCTACAGGA AAGATGGAAG AATTTTTCCT TTTCGGGTCA
    TTCTTGGAGG CCACAATGAT AGACAGAAAA ATAGGAGATA AACCCATCAG CTTTGAAGTC
    ACCATCGGTA ACTATGGTAA CCAGATTGAT GGTGTGAGCA AGCCTGCATT AGCGAAGAAG
    AAGAAGGAGG GTGGAGGAGA GAGTGAAGAA GAGGAGTCTG AGCTTATCCA CAACTCCAGC
    GAGGAAGAGG CAGAGGATGA TGGAGATCTG ACCTCTGTAC CGTCTACACC TCCTATGAAG
    CCTGTTATCA CCGACAGAAA TTACTTCCAC TTGCCTTACT TTGAAAAGAA ACCCTGCATT
    TACATCAAGA GCTGGTGGCA GGACCAGCGG AGACGTCTCT ACAACTCCAA CATAATGGAC
    AAGATAGCTG ATAAACTGGA GGAAGGTCTG AATGATGTTC AAGAGATCAT AAAAACAGAG
    AAGGCATATC CAGAACGAAG ACTAAGAGGT GTATTAGAAG AACTTAGCAC AAGCTGCAGC
    CGGTTTGTTA CACTTGCAAA CAAGGATCAG AATCTTTCTG GAAGAACCAA GCTGGACAGA
    GAGAGGCTGA AGTCCTGTAT GAGAGAAATG GAGAGTATGG GTCAGCAGGC GAAGACCATC
    CGCACACAAG TGAAAAGAAA CACAGTTCGA GATAAACTCA AGCTAGTGTT GAATTTCCTT
    CAGAGGCTTC GATTCTTAGC AGACGAGCCC CAGCACAGCA TCCCTGATGT GTTCATATGG
    ATGATTAGCA ACAACAAACG CATCGCATAC GCCCGCATAC CCTCCAAAGA CATCCTCTAC
    TCAATTGTCG ATGAAGAGAT GGGCAAAGAC TGTGGAAAAG TTAAAGCTGT TTTTCTCAGG
    CTGCCTGGAA AGAAAGGTTT TGGGCCTGCT GGCTGGACAG TTCAGGCTAA ATTAGAGATG
    TATTTGTGGC TGGGCCTGAA TAAACAGAGG AAAGACTTCC TGATTGGCCT CCCTAGTGGC
    TTTGAAGAAA ACAAGGCAGT AAAGGGAATT GGCATACAAG CTGTCCCTCC CATCAGCCTA
    GTTTATAACA TGAAGCAGGT GTTTCAGCTG AGGGCCCACA TGTATCAAGC TCGCAGTCTG
    TTTGCTGCGG ACACTAGTGG CCTGTCTGAT CCATTTGCAA GGGTTTTCTT CTCCACCCAC
    AGCCAGGTCA CGGAGGTCCT CAGTGAGACT CTCTGTCCAA CATGGGATCA GCTGCTTGTG
    TTTGATAATG TTGAACTTTA CGGTGAGGCC GGTGAACTCC GTGATGACCC TCCAATCATT
    GTCATTGAGT TGTATGATCA AGACACTGTG GGGAAAGCTG AGTTTATTGG CCGAACATTT
    GCAAAACCAC TTACTAAGAT GGTTGATGAA CACTACGGCC CACCGCGGTT TCCTCCCCAG
    CTCGAGTATT ATCAGATCTA CCGAGGAAAC TGTGCTGCGG GAGATCTATT GGCTGCTTTT
    GAGCTTCTAC AGATTCCTTA TGACGATGAG GAGATCAGAA GAGCTCTTAT TGCTGTCCAT
    GACTTTGCTG TGCCTCAGAT CAAGATTGGT CCAGCAGGGC GGGCAGCACT TCCTCCAATC
    GATGGACCGA CCGATTCTGA CCGTGGGCCC ATTCTGCCTG TTCCATTGGG AATTCGACCA
    GTTCTCAGCA GATATCGCAT CGAGGTCCTG TTTTGGGGTC TGAGGGACCT TAAAAGGGTC
    AACCTGGCTC AGGTGGACCG ACCTCGTGTG GACATTGAGT GTGCAGGGAA AGGAGTTCAG
    TCAGCCCTAA TTCAAAACTA CAAGAAGAAT CCAAACTTCA GCACATTAGT AAAGTGGTTT
    GAAGTGGATC TGCCAGAAAA TGAGCTGCTC CATCCACCAC TTAATATTCG GGTAGTGGAT
    TGCAGGGCAT TCGGACGCTA CATTTTGGTG GGGTCTCATG CTGTGACCAC CCTTCGCAAG
    TTCATCTACA GCCCCCCAGA CAAGACTGCT AACAACTGGG CTCACACAGC TAGACTGGCC
    AATGGCTACC TGGCTCTCAG CAATGGGACG TCTCATTCCC GCCCCCAATC CCGTCCTGTT
    TCTTATCCCT CAGGTGACAT TGTGGTCAAC ATGAGCCCTG AACCTAACAT TAAGAAGATG
    GACACGGTTG TCAAGATTGA AGCTACCACC GATGCGGTTG TTAAAGTTGA CTTGAATGAG
    GATGAGAAGG AGAAAGAAAA GAAGAAGAAG AAGAAGAAGA AAGGAGAAGA AGTGGAGGAA
    GAGGAGCCGG ATGAGAGCAT GCTGGACTGG TGGTCCAAAT ACTTTGCCTC AATAGAGACT
    ATGATGGAGA ATCTAAGAGC TCAGGAGGCC GCTCAGGCAG AGGCAGAGGA AAGAGAAGAT
    TTGGAGATTG CAGCAGAGAG TGCAGAGATC AAAGCTGATG ACTTTCCTAT GAAAGGCACC
    AAACCTAAAG AGAAGAGCAA AGACAAGAAG AGCACCAAGG ACAAAAAGAA GAACAACGAT
    GGCACAGAAA AACGACCTCC AAAACCAAAA GTTGATGAAC TCATGGTGTA CAATAAAGAG
    CTGGAGAGTG AGTTCGGTAG CTTTGAGGAC TGGCTTCACA CCTTCAACCT GTACCGAGGA
    AAGGCTGGAG ATGATGATGA CCACAATGTG GTTGATGAGG ACAGAATTGT GGGCAGATTC
    AAGGGCTCCC TCTGTATGTA TAAACTACCA CTGTCTGAGG AGATCACCAG AGAGGCAGGA
    TTTGATCCAA ACATGGGCAT GTTCCAGAGT ATTCCTCACA ATGACCCTAT CAACGTTCTT
    GTCAGGATTT ATATCATTCG AGCCACAGAC CTGCATCCTG CAGATATAAA TGGAAAGGCC
    GACCCTTATA TAGTCATTAA ATTGGGAAAG TCAGACATTC GAGACAAGGA GAACTACATA
    TCCAAGCAGC TGAATCCTGT CTTTGGAAAG TCATTTGATA TAGAGGCCAC GTTCCCAATG
    GAGTCTATGC TGACTGTGGC AGTGTATGAC TGGGATTTAG TCGGAACTGA TGACCTGATT
    GGTGAGACTA AGATTGATTT GGAGAACAGA TACTACAGCA AACACAGAGC CACATGTGGC
    ATTGCTTCCA ACTACTCTGT CCATGGTTAT AATGTATGGA GAGATCCTCA GAAACCAGCT
    CAGATCCTGG CTAAGTTGTG CAAAGAAGGT AAACTGGATG GACCTCACTA TGGACCTGGG
    GGAAGAGTCA AAGTTGCAAA CCGGATCTTT CTTGGGCCGA CAGAAATTGA GGATGAGAGT
    GGTCTGAAAA AACAAACAGA AGAACACTTG GCGCTCACAG TCCTTAGACA TTGGGAGGAA
    ATCCCACGTG TTGGTTGCAA ACTCATCCCT GAGCATGTGG AGACGAGGCC TCTCCTCAAC
    CCAGACAAGC CAGGCATAGA GCAGGGAAGG ATTGAAATGT GGGTGGACAT GTTTCCTATG
    GATGTCCCAG CACCAGGACC AGCCATTGAT ATATCGCCAC GCAAACCAAA GAGATATGAG
    CTCAGGGTGA TAATATGGAA TACTGACGAG GTAATTCTGG AAGACGATGA TTACTTCACA
    GGGGAAAAGT CCAGTGACAT TTTTGTGAGG GGCTGGCTTA AAGGACAGCA GGAGGACAAA
    CAGGACACCG ATGTACATTA TCACTCTCTA ACTGGAGAGG GAAACTTCAA CTGGCGCTTT
    GTCTTCCCCT TTGACTATCT CATGGCCGAG GAGAAGATTG TTATCTCCAA GAAGGAATCA
    ATGTTTTCCT GGGATGAGAC TGAGTATAAG ATCCCTGCTC GACTCACCCT TCAAGTATGG
    GATGCCGATC ACTTTTCTGC AGATGATTTT CTGGGTGCGA TTGAGTTGGA CTTGAATAAG
    TTTCCGCGTG GGGCAAAAAC AGCCAAGCAG TGCTCTCTAG ACATGGTTCT CAAAGAGCAT
    GAGCTGCCAA CCATCTCCAT CTTCAAACAA AAGAGAGTCA AGGGCTGGTG GCCATTTGTG
    GCCCAAAATG AGAATGATGA ATTTGAGCTA ACGGGAAAAG TAGAGGCAGA GCTCCATCTG
    TTGACAGCAG AGGAGGCAGA GAAGAGTCCA GTCGGCCTGG GGAGGAATGA TCCGGAGCCA
    CTTGAGAAAC CAAATCGTCC AACTACCAGC CTGTCGTGGC TGTTGAGTCC GATTCGGGTG
    ACCAGCATAA TGTGTTGGCG GCAGTATAAG TTGGTCACAG TGGTGACTCT GCTCATCATA
    ACCGGGCTGA TCTTTTTGGG CCTCCTGTTC TTTTCTCTGC CTTCAGCTTT CATGCAGAAG
    CTCTTCAACA TCTCTGGATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009293007.1
    CDS303..6443
    Translation

    Target ORF information:

    RefSeq Version XM_009294732.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio otoferlin a (otofa), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009294732.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCTTTAG TGGTGCATCT GAAGACTGTC ACGGAGCTCC GTGGGAAGGG GGACCGAATC 
    GCTAAAGTCA CTTTCAGAGG TCTCTCCTTC TTCTCTCGGG TGTTGGAAAA CTGCGAGGAT
    GAAGCTCGAT TTGAGCAGGC GTTCCGATGG CCAATTGGGA GTCAAGTGGA TGGCGACGAG
    ATGCTGGAGA TCCAAGTGTT TAATTACAGC AAAGTCTTCA CTAATAGGTT AATCGGCACA
    TTCCGCATGG TTCTTCAGAA GGTGGTGGAG GAGGGACACC TGGAGGTGTC TGACACACTC
    ATTGACGACA ATAACACAGC CATACAGACA ACCATTTCCA TAGAGATCAA ATATCAGACC
    ATGGACGGGA GTGTAAAGGT GTGGAGCGAT GGTGAATTCC TTGATATTCC AGATGACCTT
    GATGGAACCT TCCAGTTTGA GACAGATAGT CTTCTCTCAG GACGCAGCCA GAGTTCTGGA
    ACTTCACCTG GACGATCCAT TCATGGCATT CCCACATTCC GCAAGGCAGG AAAAGGAGTG
    TTCTCAGCCA TGAAGCTGGG CAAGACCCGC ACCTCTAAAG ATGACCACAA AAAAGGAGAT
    GATGCTGCCA TTCTGGATGC AGAAGATCTG GATCGAAAAG CCATGCGTCT AGGCGGCATT
    CTTGACCCTG ATACAATCTC ACTGGCCTCG GTTACTGCAG TGACCACTAA TGTCTCCAAC
    AAAAGATCAA AGCCTGACAT TAAAATGGAG CCGAGTTCAG GGCGTCCGGT TGATTATCAG
    ATCAGTGTTA CAGTAATCGA GGCCCGTCAG CTGGTCGGAT TGAACATGGA CCCTGTGGTT
    TGCGTGGAAA TTGGAGAGGA GAAGAAGTAC ACGTCAATGA AGGAGTCAAC AAACTGCCCT
    TACTACAATG AGTATTTTGT CTTCGACTTC CACGTGCCTC CAGACGTCAT GTTTGACAAA
    ATTATAAAGA TTTCAGTAAT ACATTCTAAA AACCTTCTGC GAAGCGGCAC CCTCGTGGGC
    ACCTTCAAAC TAGACGTGGG GACTGTTTAC ACACAACCTG AACATCAGTT TCATCACAAA
    TGGGCGATGC TGTCAGACCC TGATGACATC ACGACTGGCT GCAAAGGTTA TGTTAAATGT
    GACATCGCAG TCGTGGGAAA AGGAGACAAC ATCAAGACTC CACACAAGGC CAGTGAAGCA
    GACGAGGATG ACATAGAGGG GAATCTTCTC TTGCCGGAGG GTGTTCCATC AGAGAGACAG
    TGGGCTCGGT TTTATGTTAA GATTTATAGA GCTGAGGGTC TGCCTAAAAT GAACACCAGT
    ATCATGGCCA ACGTGAAGAA AGCCTTTATT GGGGAAAACA GGGACCTTGT GGACCCTTAC
    GTCCTGGTGC AATTTGCAGG GCAAAAGGGC AAAACATCTG TTCAGAAGAG CAGCTACGAG
    CCCATCTGGA ACGAGCAAGT CATCTTCACG GAGATGTTCC CACCTTTGTG TAGGCGACTG
    AAAGTTCAGA TCCGTGATTC AGACAAGGTG AACGATGTTG CCATAGGAAC CCACTTCATT
    GATCTACGAA AGGTTTCCAA TGATGGAGAC AAAGGTTTCT TGCCCACTAT GGGTCCGGCT
    TGGGTGAATA TGTATGGGTC GACCCGTAAC TACACCCTGA TGGACGAACA CCAGGACCTG
    AATGAGGGGT TGGGGGAAGG GGTGTCCTTC AGAGCACGTC TGCTCATTAG TATCGCGGTG
    GAGATTCTAG ACACCACCTC TGCTGAGATC ATGAGCTCCA CTGAAGTACA CATGGAGCCG
    GTGTCCAACA TCTCAGAGAG TGCTACAGGA AAGATGGAAG AATTTTTCCT TTTCGGGTCA
    TTCTTGGAGG CCACAATGAT AGACAGAAAA ATAGGAGATA AACCCATCAG CTTTGAAGTC
    ACCATCGGTA ACTATGGTAA CCAGATTGAT GGTGTGAGCA AGCCTGCATT AGCGAAGAAG
    AAGAAGGAGG GTGGAGGAGA GAGTGAAGAA GAGGAGTCTG AGCTTATCCA CAACTCCAGC
    GAGGAAGAGG CAGAGGATGA TGGAGATCTG ACCTCTGTAC CGTCTACACC TCCTATGAAG
    CCTGTTATCA CCGACAGAAA TTACTTCCAC TTGCCTTACT TTGAAAAGAA ACCCTGCATT
    TACATCAAGA GCTGGTGGCA GGACCAGCGG AGACGTCTCT ACAACTCCAA CATAATGGAC
    AAGATAGCTG ATAAACTGGA GGAAGGTCTG AATGATGTTC AAGAGATCAT AAAAACAGAG
    AAGGCATATC CAGAACGAAG ACTAAGAGGT GTATTAGAAG AACTTAGCAC AAGCTGCAGC
    CGGTTTGTTA CACTTGCAAA CAAGGATCAG AATCTTTCTG GAAGAACCAA GCTGGACAGA
    GAGAGGCTGA AGTCCTGTAT GAGAGAAATG GAGAGTATGG GTCAGCAGGC GAAGACCATC
    CGCACACAAG TGAAAAGAAA CACAGTTCGA GATAAACTCA AGCTAGTGTT GAATTTCCTT
    CAGAGGCTTC GATTCTTAGC AGACGAGCCC CAGCACAGCA TCCCTGATGT GTTCATATGG
    ATGATTAGCA ACAACAAACG CATCGCATAC GCCCGCATAC CCTCCAAAGA CATCCTCTAC
    TCAATTGTCG ATGAAGAGAT GGGCAAAGAC TGTGGAAAAG TTAAAGCTGT TTTTCTCAGG
    CTGCCTGGAA AGAAAGGTTT TGGGCCTGCT GGCTGGACAG TTCAGGCTAA ATTAGAGATG
    TATTTGTGGC TGGGCCTGAA TAAACAGAGG AAAGACTTCC TGATTGGCCT CCCTAGTGGC
    TTTGAAGAAA ACAAGGCAGT AAAGGGAATT GGCATACAAG CTGTCCCTCC CATCAGCCTA
    GTTTATAACA TGAAGCAGGT GTTTCAGCTG AGGGCCCACA TGTATCAAGC TCGCAGTCTG
    TTTGCTGCGG ACACTAGTGG CCTGTCTGAT CCATTTGCAA GGGTTTTCTT CTCCACCCAC
    AGCCAGGTCA CGGAGGTCCT CAGTGAGACT CTCTGTCCAA CATGGGATCA GCTGCTTGTG
    TTTGATAATG TTGAACTTTA CGGTGAGGCC GGTGAACTCC GTGATGACCC TCCAATCATT
    GTCATTGAGT TGTATGATCA AGACACTGTG GGGAAAGCTG AGTTTATTGG CCGAACATTT
    GCAAAACCAC TTACTAAGAT GGTTGATGAA CACTACGGCC CACCGCGGTT TCCTCCCCAG
    CTCGAGTATT ATCAGATCTA CCGAGGAAAC TGTGCTGCGG GAGATCTATT GGCTGCTTTT
    GAGCTTCTAC AGATTCCTTA TGACGATGAG GAGATCAGAA GAGCTCTTAT TGCTGTCCAT
    GACTTTGCTG TGCCTCAGAT CAAGATTGGT CCAGCAGGGC GGGCAGCACT TCCTCCAATC
    GATGGACCGA CCGATTCTGA CCGTGGGCCC ATTCTGCCTG TTCCATTGGG AATTCGACCA
    GTTCTCAGCA GATATCGCAT CGAGGTCCTG TTTTGGGGTC TGAGGGACCT TAAAAGGGTC
    AACCTGGCTC AGGTGGACCG ACCTCGTGTG GACATTGAGT GTGCAGGGAA AGGAGTTCAG
    TCAGCCCTAA TTCAAAACTA CAAGAAGAAT CCAAACTTCA GCACATTAGT AAAGTGGTTT
    GAAGTGGATC TGCCAGAAAA TGAGCTGCTC CATCCACCAC TTAATATTCG GGTAGTGGAT
    TGCAGGGCAT TCGGACGCTA CATTTTGGTG GGGTCTCATG CTGTGACCAC CCTTCGCAAG
    TTCATCTACA GCCCCCCAGA CAAGACTGCT AACAACTGGG CTCACACAGC TAGACTGGCC
    AATGGCTACC TGGCTCTCAG CAATGGGACG TCTCATTCCC GCCCCCAATC CCGTCCTGTT
    TCTTATCCCT CAGGTGACAT TGTGGTCAAC ATGAGCCCTG AACCTAACAT TAAGAAGATG
    GACACGGTTG TCAAGATTGA AGCTACCACC GATGCGGTTG TTAAAGTTGA CTTGAATGAG
    GATGAGAAGG AGAAAGAAAA GAAGAAGAAG AAGAAGAAGA AAGGAGAAGA AGTGGAGGAA
    GAGGAGCCGG ATGAGAGCAT GCTGGACTGG TGGTCCAAAT ACTTTGCCTC AATAGAGACT
    ATGATGGAGA ATCTAAGAGC TCAGGAGGCC GCTCAGGCAG AGGCAGAGGA AAGAGAAGAT
    TTGGAGATTG CAGCAGAGAG TGCAGAGATC AAAGCTGATG ACTTTCCTAT GAAAGGCACC
    AAACCTAAAG AGAAGAGCAA AGACAAGAAG AGCACCAAGG ACAAAAAGAA GAACAACGAT
    GGCACAGAAA AACGACCTCC AAAACCAAAA GTTGATGAAC TCATGGTGTA CAATAAAGAG
    CTGGAGAGTG AGTTCGGTAG CTTTGAGGAC TGGCTTCACA CCTTCAACCT GTACCGAGGA
    AAGGCTGGAG ATGATGATGA CCACAATGTG GTTGATGAGG ACAGAATTGT GGGCAGATTC
    AAGGGCTCCC TCTGTATGTA TAAACTACCA CTGTCTGAGG AGATCACCAG AGAGGCAGGA
    TTTGATCCAA ACATGGGCAT GTTCCAGAGT ATTCCTCACA ATGACCCTAT CAACGTTCTT
    GTCAGGATTT ATATCATTCG AGCCACAGAC CTGCATCCTG CAGATATAAA TGGAAAGGCC
    GACCCTTATA TAGTCATTAA ATTGGGAAAG TCAGACATTC GAGACAAGGA GAACTACATA
    TCCAAGCAGC TGAATCCTGT CTTTGGAAAG TCATTTGATA TAGAGGCCAC GTTCCCAATG
    GAGTCTATGC TGACTGTGGC AGTGTATGAC TGGGATTTAG TCGGAACTGA TGACCTGATT
    GGTGAGACTA AGATTGATTT GGAGAACAGA TACTACAGCA AACACAGAGC CACATGTGGC
    ATTGCTTCCA ACTACTCTGT CCATGGTTAT AATGTATGGA GAGATCCTCA GAAACCAGCT
    CAGATCCTGG CTAAGTTGTG CAAAGAAGGT AAACTGGATG GACCTCACTA TGGACCTGGG
    GGAAGAGTCA AAGTTGCAAA CCGGATCTTT CTTGGGCCGA CAGAAATTGA GGATGAGAGT
    GGTCTGAAAA AACAAACAGA AGAACACTTG GCGCTCACAG TCCTTAGACA TTGGGAGGAA
    ATCCCACGTG TTGGTTGCAA ACTCATCCCT GAGCATGTGG AGACGAGGCC TCTCCTCAAC
    CCAGACAAGC CAGGCATAGA GCAGGGAAGG ATTGAAATGT GGGTGGACAT GTTTCCTATG
    GATGTCCCAG CACCAGGACC AGCCATTGAT ATATCGCCAC GCAAACCAAA GAGATATGAG
    CTCAGGGTGA TAATATGGAA TACTGACGAG GTAATTCTGG AAGACGATGA TTACTTCACA
    GGGGAAAAGT CCAGTGACAT TTTTGTGAGG GGCTGGCTTA AAGGACAGCA GGAGGACAAA
    CAGGACACCG ATGTACATTA TCACTCTCTA ACTGGAGAGG GAAACTTCAA CTGGCGCTTT
    GTCTTCCCCT TTGACTATCT CATGGCCGAG GAGAAGATTG TTATCTCCAA GAAGGAATCA
    ATGTTTTCCT GGGATGAGAC TGAGTATAAG ATCCCTGCTC GACTCACCCT TCAAGTATGG
    GATGCCGATC ACTTTTCTGC AGATGATTTT CTGGGTGCGA TTGAGTTGGA CTTGAATAAG
    TTTCCGCGTG GGGCAAAAAC AGCCAAGCAG TGCTCTCTAG ACATGGTTCT CAAAGAGCAT
    GAGCTGCCAA CCATCTCCAT CTTCAAACAA AAGAGAGTCA AGGGCTGGTG GCCATTTGTG
    GCCCAAAATG AGAATGATGA ATTTGAGCTA ACGGGAAAAG TAGAGGCAGA GCTCCATCTG
    TTGACAGCAG AGGAGGCAGA GAAGAGTCCA GTCGGCCTGG GGAGGAATGA TCCGGAGCCA
    CTTGAGAAAC CAAATCGTCC AACTACCAGC CTGTCGTGGC TGTTGAGTCC GATTCGGGTG
    ACCAGCATAA TGTGTTGGCG GCAGTATAAG TTGGTCACAG TGGTGACTCT GCTCATCATA
    ACCGGGCTGA TCTTTTTGGG CCTCCTGTTC TTTTCTCTGC CTTCAGCTTT CATGCAGAAG
    CTCTTCAACA TCTCTGGATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42604
    Clone ID Related Accession (Same CDS sequence) XM_009294732.2 , XM_009294732.3
    Accession Version XM_009294732.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6141bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product otoferlin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009294732.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCTTTAG TGGTGCATCT GAAGACTGTC ACGGAGCTCC GTGGGAAGGG GGACCGAATC 
    GCTAAAGTCA CTTTCAGAGG TCTCTCCTTC TTCTCTCGGG TGTTGGAAAA CTGCGAGGAT
    GAAGCTCGAT TTGAGCAGGC GTTCCGATGG CCAATTGGGA GTCAAGTGGA TGGCGACGAG
    ATGCTGGAGA TCCAAGTGTT TAATTACAGC AAAGTCTTCA CTAATAGGTT AATCGGCACA
    TTCCGCATGG TTCTTCAGAA GGTGGTGGAG GAGGGACACC TGGAGGTGTC TGACACACTC
    ATTGACGACA ATAACACAGC CATACAGACA ACCATTTCCA TAGAGATCAA ATATCAGACC
    ATGGACGGGA GTGTAAAGGT GTGGAGCGAT GGTGAATTCC TTGATATTCC AGATGACCTT
    GATGGAACCT TCCAGTTTGA GACAGATAGT CTTCTCTCAG GACGCAGCCA GAGTTCTGGA
    ACTTCACCTG GACGATCCAT TCATGGCATT CCCACATTCC GCAAGGCAGG AAAAGGAGTG
    TTCTCAGCCA TGAAGCTGGG CAAGACCCGC ACCTCTAAAG ATGACCACAA AAAAGGAGAT
    GATGCTGCCA TTCTGGATGC AGAAGATCTG GATCGAAAAG CCATGCGTCT AGGCGGCATT
    CTTGACCCTG ATACAATCTC ACTGGCCTCG GTTACTGCAG TGACCACTAA TGTCTCCAAC
    AAAAGATCAA AGCCTGACAT TAAAATGGAG CCGAGTTCAG GGCGTCCGGT TGATTATCAG
    ATCAGTGTTA CAGTAATCGA GGCCCGTCAG CTGGTCGGAT TGAACATGGA CCCTGTGGTT
    TGCGTGGAAA TTGGAGAGGA GAAGAAGTAC ACGTCAATGA AGGAGTCAAC AAACTGCCCT
    TACTACAATG AGTATTTTGT CTTCGACTTC CACGTGCCTC CAGACGTCAT GTTTGACAAA
    ATTATAAAGA TTTCAGTAAT ACATTCTAAA AACCTTCTGC GAAGCGGCAC CCTCGTGGGC
    ACCTTCAAAC TAGACGTGGG GACTGTTTAC ACACAACCTG AACATCAGTT TCATCACAAA
    TGGGCGATGC TGTCAGACCC TGATGACATC ACGACTGGCT GCAAAGGTTA TGTTAAATGT
    GACATCGCAG TCGTGGGAAA AGGAGACAAC ATCAAGACTC CACACAAGGC CAGTGAAGCA
    GACGAGGATG ACATAGAGGG GAATCTTCTC TTGCCGGAGG GTGTTCCATC AGAGAGACAG
    TGGGCTCGGT TTTATGTTAA GATTTATAGA GCTGAGGGTC TGCCTAAAAT GAACACCAGT
    ATCATGGCCA ACGTGAAGAA AGCCTTTATT GGGGAAAACA GGGACCTTGT GGACCCTTAC
    GTCCTGGTGC AATTTGCAGG GCAAAAGGGC AAAACATCTG TTCAGAAGAG CAGCTACGAG
    CCCATCTGGA ACGAGCAAGT CATCTTCACG GAGATGTTCC CACCTTTGTG TAGGCGACTG
    AAAGTTCAGA TCCGTGATTC AGACAAGGTG AACGATGTTG CCATAGGAAC CCACTTCATT
    GATCTACGAA AGGTTTCCAA TGATGGAGAC AAAGGTTTCT TGCCCACTAT GGGTCCGGCT
    TGGGTGAATA TGTATGGGTC GACCCGTAAC TACACCCTGA TGGACGAACA CCAGGACCTG
    AATGAGGGGT TGGGGGAAGG GGTGTCCTTC AGAGCACGTC TGCTCATTAG TATCGCGGTG
    GAGATTCTAG ACACCACCTC TGCTGAGATC ATGAGCTCCA CTGAAGTACA CATGGAGCCG
    GTGTCCAACA TCTCAGAGAG TGCTACAGGA AAGATGGAAG AATTTTTCCT TTTCGGGTCA
    TTCTTGGAGG CCACAATGAT AGACAGAAAA ATAGGAGATA AACCCATCAG CTTTGAAGTC
    ACCATCGGTA ACTATGGTAA CCAGATTGAT GGTGTGAGCA AGCCTGCATT AGCGAAGAAG
    AAGAAGGAGG GTGGAGGAGA GAGTGAAGAA GAGGAGTCTG AGCTTATCCA CAACTCCAGC
    GAGGAAGAGG CAGAGGATGA TGGAGATCTG ACCTCTGTAC CGTCTACACC TCCTATGAAG
    CCTGTTATCA CCGACAGAAA TTACTTCCAC TTGCCTTACT TTGAAAAGAA ACCCTGCATT
    TACATCAAGA GCTGGTGGCA GGACCAGCGG AGACGTCTCT ACAACTCCAA CATAATGGAC
    AAGATAGCTG ATAAACTGGA GGAAGGTCTG AATGATGTTC AAGAGATCAT AAAAACAGAG
    AAGGCATATC CAGAACGAAG ACTAAGAGGT GTATTAGAAG AACTTAGCAC AAGCTGCAGC
    CGGTTTGTTA CACTTGCAAA CAAGGATCAG AATCTTTCTG GAAGAACCAA GCTGGACAGA
    GAGAGGCTGA AGTCCTGTAT GAGAGAAATG GAGAGTATGG GTCAGCAGGC GAAGACCATC
    CGCACACAAG TGAAAAGAAA CACAGTTCGA GATAAACTCA AGCTAGTGTT GAATTTCCTT
    CAGAGGCTTC GATTCTTAGC AGACGAGCCC CAGCACAGCA TCCCTGATGT GTTCATATGG
    ATGATTAGCA ACAACAAACG CATCGCATAC GCCCGCATAC CCTCCAAAGA CATCCTCTAC
    TCAATTGTCG ATGAAGAGAT GGGCAAAGAC TGTGGAAAAG TTAAAGCTGT TTTTCTCAGG
    CTGCCTGGAA AGAAAGGTTT TGGGCCTGCT GGCTGGACAG TTCAGGCTAA ATTAGAGATG
    TATTTGTGGC TGGGCCTGAA TAAACAGAGG AAAGACTTCC TGATTGGCCT CCCTAGTGGC
    TTTGAAGAAA ACAAGGCAGT AAAGGGAATT GGCATACAAG CTGTCCCTCC CATCAGCCTA
    GTTTATAACA TGAAGCAGGT GTTTCAGCTG AGGGCCCACA TGTATCAAGC TCGCAGTCTG
    TTTGCTGCGG ACACTAGTGG CCTGTCTGAT CCATTTGCAA GGGTTTTCTT CTCCACCCAC
    AGCCAGGTCA CGGAGGTCCT CAGTGAGACT CTCTGTCCAA CATGGGATCA GCTGCTTGTG
    TTTGATAATG TTGAACTTTA CGGTGAGGCC GGTGAACTCC GTGATGACCC TCCAATCATT
    GTCATTGAGT TGTATGATCA AGACACTGTG GGGAAAGCTG AGTTTATTGG CCGAACATTT
    GCAAAACCAC TTACTAAGAT GGTTGATGAA CACTACGGCC CACCGCGGTT TCCTCCCCAG
    CTCGAGTATT ATCAGATCTA CCGAGGAAAC TGTGCTGCGG GAGATCTATT GGCTGCTTTT
    GAGCTTCTAC AGATTCCTTA TGACGATGAG GAGATCAGAA GAGCTCTTAT TGCTGTCCAT
    GACTTTGCTG TGCCTCAGAT CAAGATTGGT CCAGCAGGGC GGGCAGCACT TCCTCCAATC
    GATGGACCGA CCGATTCTGA CCGTGGGCCC ATTCTGCCTG TTCCATTGGG AATTCGACCA
    GTTCTCAGCA GATATCGCAT CGAGGTCCTG TTTTGGGGTC TGAGGGACCT TAAAAGGGTC
    AACCTGGCTC AGGTGGACCG ACCTCGTGTG GACATTGAGT GTGCAGGGAA AGGAGTTCAG
    TCAGCCCTAA TTCAAAACTA CAAGAAGAAT CCAAACTTCA GCACATTAGT AAAGTGGTTT
    GAAGTGGATC TGCCAGAAAA TGAGCTGCTC CATCCACCAC TTAATATTCG GGTAGTGGAT
    TGCAGGGCAT TCGGACGCTA CATTTTGGTG GGGTCTCATG CTGTGACCAC CCTTCGCAAG
    TTCATCTACA GCCCCCCAGA CAAGACTGCT AACAACTGGG CTCACACAGC TAGACTGGCC
    AATGGCTACC TGGCTCTCAG CAATGGGACG TCTCATTCCC GCCCCCAATC CCGTCCTGTT
    TCTTATCCCT CAGGTGACAT TGTGGTCAAC ATGAGCCCTG AACCTAACAT TAAGAAGATG
    GACACGGTTG TCAAGATTGA AGCTACCACC GATGCGGTTG TTAAAGTTGA CTTGAATGAG
    GATGAGAAGG AGAAAGAAAA GAAGAAGAAG AAGAAGAAGA AAGGAGAAGA AGTGGAGGAA
    GAGGAGCCGG ATGAGAGCAT GCTGGACTGG TGGTCCAAAT ACTTTGCCTC AATAGAGACT
    ATGATGGAGA ATCTAAGAGC TCAGGAGGCC GCTCAGGCAG AGGCAGAGGA AAGAGAAGAT
    TTGGAGATTG CAGCAGAGAG TGCAGAGATC AAAGCTGATG ACTTTCCTAT GAAAGGCACC
    AAACCTAAAG AGAAGAGCAA AGACAAGAAG AGCACCAAGG ACAAAAAGAA GAACAACGAT
    GGCACAGAAA AACGACCTCC AAAACCAAAA GTTGATGAAC TCATGGTGTA CAATAAAGAG
    CTGGAGAGTG AGTTCGGTAG CTTTGAGGAC TGGCTTCACA CCTTCAACCT GTACCGAGGA
    AAGGCTGGAG ATGATGATGA CCACAATGTG GTTGATGAGG ACAGAATTGT GGGCAGATTC
    AAGGGCTCCC TCTGTATGTA TAAACTACCA CTGTCTGAGG AGATCACCAG AGAGGCAGGA
    TTTGATCCAA ACATGGGCAT GTTCCAGAGT ATTCCTCACA ATGACCCTAT CAACGTTCTT
    GTCAGGATTT ATATCATTCG AGCCACAGAC CTGCATCCTG CAGATATAAA TGGAAAGGCC
    GACCCTTATA TAGTCATTAA ATTGGGAAAG TCAGACATTC GAGACAAGGA GAACTACATA
    TCCAAGCAGC TGAATCCTGT CTTTGGAAAG TCATTTGATA TAGAGGCCAC GTTCCCAATG
    GAGTCTATGC TGACTGTGGC AGTGTATGAC TGGGATTTAG TCGGAACTGA TGACCTGATT
    GGTGAGACTA AGATTGATTT GGAGAACAGA TACTACAGCA AACACAGAGC CACATGTGGC
    ATTGCTTCCA ACTACTCTGT CCATGGTTAT AATGTATGGA GAGATCCTCA GAAACCAGCT
    CAGATCCTGG CTAAGTTGTG CAAAGAAGGT AAACTGGATG GACCTCACTA TGGACCTGGG
    GGAAGAGTCA AAGTTGCAAA CCGGATCTTT CTTGGGCCGA CAGAAATTGA GGATGAGAGT
    GGTCTGAAAA AACAAACAGA AGAACACTTG GCGCTCACAG TCCTTAGACA TTGGGAGGAA
    ATCCCACGTG TTGGTTGCAA ACTCATCCCT GAGCATGTGG AGACGAGGCC TCTCCTCAAC
    CCAGACAAGC CAGGCATAGA GCAGGGAAGG ATTGAAATGT GGGTGGACAT GTTTCCTATG
    GATGTCCCAG CACCAGGACC AGCCATTGAT ATATCGCCAC GCAAACCAAA GAGATATGAG
    CTCAGGGTGA TAATATGGAA TACTGACGAG GTAATTCTGG AAGACGATGA TTACTTCACA
    GGGGAAAAGT CCAGTGACAT TTTTGTGAGG GGCTGGCTTA AAGGACAGCA GGAGGACAAA
    CAGGACACCG ATGTACATTA TCACTCTCTA ACTGGAGAGG GAAACTTCAA CTGGCGCTTT
    GTCTTCCCCT TTGACTATCT CATGGCCGAG GAGAAGATTG TTATCTCCAA GAAGGAATCA
    ATGTTTTCCT GGGATGAGAC TGAGTATAAG ATCCCTGCTC GACTCACCCT TCAAGTATGG
    GATGCCGATC ACTTTTCTGC AGATGATTTT CTGGGTGCGA TTGAGTTGGA CTTGAATAAG
    TTTCCGCGTG GGGCAAAAAC AGCCAAGCAG TGCTCTCTAG ACATGGTTCT CAAAGAGCAT
    GAGCTGCCAA CCATCTCCAT CTTCAAACAA AAGAGAGTCA AGGGCTGGTG GCCATTTGTG
    GCCCAAAATG AGAATGATGA ATTTGAGCTA ACGGGAAAAG TAGAGGCAGA GCTCCATCTG
    TTGACAGCAG AGGAGGCAGA GAAGAGTCCA GTCGGCCTGG GGAGGAATGA TCCGGAGCCA
    CTTGAGAAAC CAAATCGTCC AACTACCAGC CTGTCGTGGC TGTTGAGTCC GATTCGGGTG
    ACCAGCATAA TGTGTTGGCG GCAGTATAAG TTGGTCACAG TGGTGACTCT GCTCATCATA
    ACCGGGCTGA TCTTTTTGGG CCTCCTGTTC TTTTCTCTGC CTTCAGCTTT CATGCAGAAG
    CTCTTCAACA TCTCTGGATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009293007.1
    CDS300..6440
    Translation

    Target ORF information:

    RefSeq Version XM_009294732.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio otoferlin a (otofa), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009294732.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCTTTAG TGGTGCATCT GAAGACTGTC ACGGAGCTCC GTGGGAAGGG GGACCGAATC 
    GCTAAAGTCA CTTTCAGAGG TCTCTCCTTC TTCTCTCGGG TGTTGGAAAA CTGCGAGGAT
    GAAGCTCGAT TTGAGCAGGC GTTCCGATGG CCAATTGGGA GTCAAGTGGA TGGCGACGAG
    ATGCTGGAGA TCCAAGTGTT TAATTACAGC AAAGTCTTCA CTAATAGGTT AATCGGCACA
    TTCCGCATGG TTCTTCAGAA GGTGGTGGAG GAGGGACACC TGGAGGTGTC TGACACACTC
    ATTGACGACA ATAACACAGC CATACAGACA ACCATTTCCA TAGAGATCAA ATATCAGACC
    ATGGACGGGA GTGTAAAGGT GTGGAGCGAT GGTGAATTCC TTGATATTCC AGATGACCTT
    GATGGAACCT TCCAGTTTGA GACAGATAGT CTTCTCTCAG GACGCAGCCA GAGTTCTGGA
    ACTTCACCTG GACGATCCAT TCATGGCATT CCCACATTCC GCAAGGCAGG AAAAGGAGTG
    TTCTCAGCCA TGAAGCTGGG CAAGACCCGC ACCTCTAAAG ATGACCACAA AAAAGGAGAT
    GATGCTGCCA TTCTGGATGC AGAAGATCTG GATCGAAAAG CCATGCGTCT AGGCGGCATT
    CTTGACCCTG ATACAATCTC ACTGGCCTCG GTTACTGCAG TGACCACTAA TGTCTCCAAC
    AAAAGATCAA AGCCTGACAT TAAAATGGAG CCGAGTTCAG GGCGTCCGGT TGATTATCAG
    ATCAGTGTTA CAGTAATCGA GGCCCGTCAG CTGGTCGGAT TGAACATGGA CCCTGTGGTT
    TGCGTGGAAA TTGGAGAGGA GAAGAAGTAC ACGTCAATGA AGGAGTCAAC AAACTGCCCT
    TACTACAATG AGTATTTTGT CTTCGACTTC CACGTGCCTC CAGACGTCAT GTTTGACAAA
    ATTATAAAGA TTTCAGTAAT ACATTCTAAA AACCTTCTGC GAAGCGGCAC CCTCGTGGGC
    ACCTTCAAAC TAGACGTGGG GACTGTTTAC ACACAACCTG AACATCAGTT TCATCACAAA
    TGGGCGATGC TGTCAGACCC TGATGACATC ACGACTGGCT GCAAAGGTTA TGTTAAATGT
    GACATCGCAG TCGTGGGAAA AGGAGACAAC ATCAAGACTC CACACAAGGC CAGTGAAGCA
    GACGAGGATG ACATAGAGGG GAATCTTCTC TTGCCGGAGG GTGTTCCATC AGAGAGACAG
    TGGGCTCGGT TTTATGTTAA GATTTATAGA GCTGAGGGTC TGCCTAAAAT GAACACCAGT
    ATCATGGCCA ACGTGAAGAA AGCCTTTATT GGGGAAAACA GGGACCTTGT GGACCCTTAC
    GTCCTGGTGC AATTTGCAGG GCAAAAGGGC AAAACATCTG TTCAGAAGAG CAGCTACGAG
    CCCATCTGGA ACGAGCAAGT CATCTTCACG GAGATGTTCC CACCTTTGTG TAGGCGACTG
    AAAGTTCAGA TCCGTGATTC AGACAAGGTG AACGATGTTG CCATAGGAAC CCACTTCATT
    GATCTACGAA AGGTTTCCAA TGATGGAGAC AAAGGTTTCT TGCCCACTAT GGGTCCGGCT
    TGGGTGAATA TGTATGGGTC GACCCGTAAC TACACCCTGA TGGACGAACA CCAGGACCTG
    AATGAGGGGT TGGGGGAAGG GGTGTCCTTC AGAGCACGTC TGCTCATTAG TATCGCGGTG
    GAGATTCTAG ACACCACCTC TGCTGAGATC ATGAGCTCCA CTGAAGTACA CATGGAGCCG
    GTGTCCAACA TCTCAGAGAG TGCTACAGGA AAGATGGAAG AATTTTTCCT TTTCGGGTCA
    TTCTTGGAGG CCACAATGAT AGACAGAAAA ATAGGAGATA AACCCATCAG CTTTGAAGTC
    ACCATCGGTA ACTATGGTAA CCAGATTGAT GGTGTGAGCA AGCCTGCATT AGCGAAGAAG
    AAGAAGGAGG GTGGAGGAGA GAGTGAAGAA GAGGAGTCTG AGCTTATCCA CAACTCCAGC
    GAGGAAGAGG CAGAGGATGA TGGAGATCTG ACCTCTGTAC CGTCTACACC TCCTATGAAG
    CCTGTTATCA CCGACAGAAA TTACTTCCAC TTGCCTTACT TTGAAAAGAA ACCCTGCATT
    TACATCAAGA GCTGGTGGCA GGACCAGCGG AGACGTCTCT ACAACTCCAA CATAATGGAC
    AAGATAGCTG ATAAACTGGA GGAAGGTCTG AATGATGTTC AAGAGATCAT AAAAACAGAG
    AAGGCATATC CAGAACGAAG ACTAAGAGGT GTATTAGAAG AACTTAGCAC AAGCTGCAGC
    CGGTTTGTTA CACTTGCAAA CAAGGATCAG AATCTTTCTG GAAGAACCAA GCTGGACAGA
    GAGAGGCTGA AGTCCTGTAT GAGAGAAATG GAGAGTATGG GTCAGCAGGC GAAGACCATC
    CGCACACAAG TGAAAAGAAA CACAGTTCGA GATAAACTCA AGCTAGTGTT GAATTTCCTT
    CAGAGGCTTC GATTCTTAGC AGACGAGCCC CAGCACAGCA TCCCTGATGT GTTCATATGG
    ATGATTAGCA ACAACAAACG CATCGCATAC GCCCGCATAC CCTCCAAAGA CATCCTCTAC
    TCAATTGTCG ATGAAGAGAT GGGCAAAGAC TGTGGAAAAG TTAAAGCTGT TTTTCTCAGG
    CTGCCTGGAA AGAAAGGTTT TGGGCCTGCT GGCTGGACAG TTCAGGCTAA ATTAGAGATG
    TATTTGTGGC TGGGCCTGAA TAAACAGAGG AAAGACTTCC TGATTGGCCT CCCTAGTGGC
    TTTGAAGAAA ACAAGGCAGT AAAGGGAATT GGCATACAAG CTGTCCCTCC CATCAGCCTA
    GTTTATAACA TGAAGCAGGT GTTTCAGCTG AGGGCCCACA TGTATCAAGC TCGCAGTCTG
    TTTGCTGCGG ACACTAGTGG CCTGTCTGAT CCATTTGCAA GGGTTTTCTT CTCCACCCAC
    AGCCAGGTCA CGGAGGTCCT CAGTGAGACT CTCTGTCCAA CATGGGATCA GCTGCTTGTG
    TTTGATAATG TTGAACTTTA CGGTGAGGCC GGTGAACTCC GTGATGACCC TCCAATCATT
    GTCATTGAGT TGTATGATCA AGACACTGTG GGGAAAGCTG AGTTTATTGG CCGAACATTT
    GCAAAACCAC TTACTAAGAT GGTTGATGAA CACTACGGCC CACCGCGGTT TCCTCCCCAG
    CTCGAGTATT ATCAGATCTA CCGAGGAAAC TGTGCTGCGG GAGATCTATT GGCTGCTTTT
    GAGCTTCTAC AGATTCCTTA TGACGATGAG GAGATCAGAA GAGCTCTTAT TGCTGTCCAT
    GACTTTGCTG TGCCTCAGAT CAAGATTGGT CCAGCAGGGC GGGCAGCACT TCCTCCAATC
    GATGGACCGA CCGATTCTGA CCGTGGGCCC ATTCTGCCTG TTCCATTGGG AATTCGACCA
    GTTCTCAGCA GATATCGCAT CGAGGTCCTG TTTTGGGGTC TGAGGGACCT TAAAAGGGTC
    AACCTGGCTC AGGTGGACCG ACCTCGTGTG GACATTGAGT GTGCAGGGAA AGGAGTTCAG
    TCAGCCCTAA TTCAAAACTA CAAGAAGAAT CCAAACTTCA GCACATTAGT AAAGTGGTTT
    GAAGTGGATC TGCCAGAAAA TGAGCTGCTC CATCCACCAC TTAATATTCG GGTAGTGGAT
    TGCAGGGCAT TCGGACGCTA CATTTTGGTG GGGTCTCATG CTGTGACCAC CCTTCGCAAG
    TTCATCTACA GCCCCCCAGA CAAGACTGCT AACAACTGGG CTCACACAGC TAGACTGGCC
    AATGGCTACC TGGCTCTCAG CAATGGGACG TCTCATTCCC GCCCCCAATC CCGTCCTGTT
    TCTTATCCCT CAGGTGACAT TGTGGTCAAC ATGAGCCCTG AACCTAACAT TAAGAAGATG
    GACACGGTTG TCAAGATTGA AGCTACCACC GATGCGGTTG TTAAAGTTGA CTTGAATGAG
    GATGAGAAGG AGAAAGAAAA GAAGAAGAAG AAGAAGAAGA AAGGAGAAGA AGTGGAGGAA
    GAGGAGCCGG ATGAGAGCAT GCTGGACTGG TGGTCCAAAT ACTTTGCCTC AATAGAGACT
    ATGATGGAGA ATCTAAGAGC TCAGGAGGCC GCTCAGGCAG AGGCAGAGGA AAGAGAAGAT
    TTGGAGATTG CAGCAGAGAG TGCAGAGATC AAAGCTGATG ACTTTCCTAT GAAAGGCACC
    AAACCTAAAG AGAAGAGCAA AGACAAGAAG AGCACCAAGG ACAAAAAGAA GAACAACGAT
    GGCACAGAAA AACGACCTCC AAAACCAAAA GTTGATGAAC TCATGGTGTA CAATAAAGAG
    CTGGAGAGTG AGTTCGGTAG CTTTGAGGAC TGGCTTCACA CCTTCAACCT GTACCGAGGA
    AAGGCTGGAG ATGATGATGA CCACAATGTG GTTGATGAGG ACAGAATTGT GGGCAGATTC
    AAGGGCTCCC TCTGTATGTA TAAACTACCA CTGTCTGAGG AGATCACCAG AGAGGCAGGA
    TTTGATCCAA ACATGGGCAT GTTCCAGAGT ATTCCTCACA ATGACCCTAT CAACGTTCTT
    GTCAGGATTT ATATCATTCG AGCCACAGAC CTGCATCCTG CAGATATAAA TGGAAAGGCC
    GACCCTTATA TAGTCATTAA ATTGGGAAAG TCAGACATTC GAGACAAGGA GAACTACATA
    TCCAAGCAGC TGAATCCTGT CTTTGGAAAG TCATTTGATA TAGAGGCCAC GTTCCCAATG
    GAGTCTATGC TGACTGTGGC AGTGTATGAC TGGGATTTAG TCGGAACTGA TGACCTGATT
    GGTGAGACTA AGATTGATTT GGAGAACAGA TACTACAGCA AACACAGAGC CACATGTGGC
    ATTGCTTCCA ACTACTCTGT CCATGGTTAT AATGTATGGA GAGATCCTCA GAAACCAGCT
    CAGATCCTGG CTAAGTTGTG CAAAGAAGGT AAACTGGATG GACCTCACTA TGGACCTGGG
    GGAAGAGTCA AAGTTGCAAA CCGGATCTTT CTTGGGCCGA CAGAAATTGA GGATGAGAGT
    GGTCTGAAAA AACAAACAGA AGAACACTTG GCGCTCACAG TCCTTAGACA TTGGGAGGAA
    ATCCCACGTG TTGGTTGCAA ACTCATCCCT GAGCATGTGG AGACGAGGCC TCTCCTCAAC
    CCAGACAAGC CAGGCATAGA GCAGGGAAGG ATTGAAATGT GGGTGGACAT GTTTCCTATG
    GATGTCCCAG CACCAGGACC AGCCATTGAT ATATCGCCAC GCAAACCAAA GAGATATGAG
    CTCAGGGTGA TAATATGGAA TACTGACGAG GTAATTCTGG AAGACGATGA TTACTTCACA
    GGGGAAAAGT CCAGTGACAT TTTTGTGAGG GGCTGGCTTA AAGGACAGCA GGAGGACAAA
    CAGGACACCG ATGTACATTA TCACTCTCTA ACTGGAGAGG GAAACTTCAA CTGGCGCTTT
    GTCTTCCCCT TTGACTATCT CATGGCCGAG GAGAAGATTG TTATCTCCAA GAAGGAATCA
    ATGTTTTCCT GGGATGAGAC TGAGTATAAG ATCCCTGCTC GACTCACCCT TCAAGTATGG
    GATGCCGATC ACTTTTCTGC AGATGATTTT CTGGGTGCGA TTGAGTTGGA CTTGAATAAG
    TTTCCGCGTG GGGCAAAAAC AGCCAAGCAG TGCTCTCTAG ACATGGTTCT CAAAGAGCAT
    GAGCTGCCAA CCATCTCCAT CTTCAAACAA AAGAGAGTCA AGGGCTGGTG GCCATTTGTG
    GCCCAAAATG AGAATGATGA ATTTGAGCTA ACGGGAAAAG TAGAGGCAGA GCTCCATCTG
    TTGACAGCAG AGGAGGCAGA GAAGAGTCCA GTCGGCCTGG GGAGGAATGA TCCGGAGCCA
    CTTGAGAAAC CAAATCGTCC AACTACCAGC CTGTCGTGGC TGTTGAGTCC GATTCGGGTG
    ACCAGCATAA TGTGTTGGCG GCAGTATAAG TTGGTCACAG TGGTGACTCT GCTCATCATA
    ACCGGGCTGA TCTTTTTGGG CCTCCTGTTC TTTTCTCTGC CTTCAGCTTT CATGCAGAAG
    CTCTTCAACA TCTCTGGATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42605
    Clone ID Related Accession (Same CDS sequence) XM_009294733.2 , XM_009294733.3
    Accession Version XM_009294733.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6141bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product otoferlin isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009294733.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCTTTAG TGGTGCATCT GAAGACTGTC ACGGAGCTCC GTGGGAAGGG GGACCGAATC 
    GCTAAAGTCA CTTTCAGAGG TCTCTCCTTC TTCTCTCGGG TGTTGGAAAA CTGCGAGGAT
    GAAGCTCGAT TTGAGCAGGC GTTCCGATGG CCAATTGGGA GTCAAGTGGA TGGCGACGAG
    ATGCTGGAGA TCCAAGTGTT TAATTACAGC AAAGTCTTCA CTAATAGGTT AATCGGCACA
    TTCCGCATGG TTCTTCAGAA GGTGGTGGAG GAGGGACACC TGGAGGTGTC TGACACACTC
    ATTGACGACA ATAACACAGC CATACAGACA ACCATTTCCA TAGAGATCAA ATATCAGACC
    ATGGACGGGA GTGTAAAGGT GTGGAGCGAT GGTGAATTCC TTGATATTCC AGATGACCTT
    GATGGAACCT TCCAGTTTGA GACAGATAGT CTTCTCTCAG GACGCAGCCA GAGTTCTGGA
    ACTTCACCTG GACGATCCAT TCATGGCATT CCCACATTCC GCAAGGCAGG AAAAGGAGTG
    TTCTCAGCCA TGAAGCTGGG CAAGACCCGC ACCTCTAAAG ATGACCACAA AAAAGGAGAT
    GATGCTGCCA TTCTGGATGC AGAAGATCTG GATCGAAAAG CCATGCGTCT AGGCGGCATT
    CTTGACCCTG ATACAATCTC ACTGGCCTCG GTTACTGCAG TGACCACTAA TGTCTCCAAC
    AAAAGATCAA AGCCTGACAT TAAAATGGAG CCGAGTTCAG GGCGTCCGGT TGATTATCAG
    ATCAGTGTTA CAGTAATCGA GGCCCGTCAG CTGGTCGGAT TGAACATGGA CCCTGTGGTT
    TGCGTGGAAA TTGGAGAGGA GAAGAAGTAC ACGTCAATGA AGGAGTCAAC AAACTGCCCT
    TACTACAATG AGTATTTTGT CTTCGACTTC CACGTGCCTC CAGACGTCAT GTTTGACAAA
    ATTATAAAGA TTTCAGTAAT ACATTCTAAA AACCTTCTGC GAAGCGGCAC CCTCGTGGGC
    ACCTTCAAAC TAGACGTGGG GACTGTTTAC ACACAACCTG AACATCAGTT TCATCACAAA
    TGGGCGATGC TGTCAGACCC TGATGACATC ACGACTGGCT GCAAAGGTTA TGTTAAATGT
    GACATCGCAG TCGTGGGAAA AGGAGACAAC ATCAAGACTC CACACAAGGC CAGTGAAGCA
    GACGAGGATG ACATAGAGGG GAATCTTCTC TTGCCGGAGG GTGTTCCATC AGAGAGACAG
    TGGGCTCGGT TTTATGTTAA GATTTATAGA GCTGAGGGTC TGCCTAAAAT GAACACCAGT
    ATCATGGCCA ACGTGAAGAA AGCCTTTATT GGGGAAAACA GGGACCTTGT GGACCCTTAC
    GTCCTGGTGC AATTTGCAGG GCAAAAGGGC AAAACATCTG TTCAGAAGAG CAGCTACGAG
    CCCATCTGGA ACGAGCAAGT CATCTTCACG GAGATGTTCC CACCTTTGTG TAGGCGACTG
    AAAGTTCAGA TCCGTGATTC AGACAAGGTG AACGATGTTG CCATAGGAAC CCACTTCATT
    GATCTACGAA AGGTTTCCAA TGATGGAGAC AAAGGTTTCT TGCCCACTAT GGGTCCGGCT
    TGGGTGAATA TGTATGGGTC GACCCGTAAC TACACCCTGA TGGACGAACA CCAGGACCTG
    AATGAGGGGT TGGGGGAAGG GGTGTCCTTC AGAGCACGTC TGCTCATTAG TATCGCGGTG
    GAGATTCTAG ACACCACCTC TGCTGAGATC ATGAGCTCCA CTGAAGTACA CATGGAGCCG
    GTGTCCAACA TCTCAGAGAG TGCTACAGGA AAGATGGAAG AATTTTTCCT TTTCGGGTCA
    TTCTTGGAGG CCACAATGAT AGACAGAAAA ATAGGAGATA AACCCATCAG CTTTGAAGTC
    ACCATCGGTA ACTATGGTAA CCAGATTGAT GGTGTGAGCA AGCCTGCATT AGCGAAGAAG
    AAGAAGGAGG GTGGAGGAGA GAGTGAAGAA GAGGAGTCTG AGCTTATCCA CAACTCCAGC
    GAGGAAGAGG CAGAGGATGA TGGAGATCTG ACCTCTGTAC CGTCTACACC TCCTATGAAG
    CCTGTTATCA CCGACAGAAA TTACTTCCAC TTGCCTTACT TTGAAAAGAA ACCCTGCATT
    TACATCAAGA GCTGGTGGCA GGACCAGCGG AGACGTCTCT ACAACTCCAA CATAATGGAC
    AAGATAGCTG ATAAACTGGA GGAAGGTCTG AATGATGTTC AAGAGATCAT AAAAACAGAG
    AAGGCATATC CAGAACGAAG ACTAAGAGGT GTATTAGAAG AACTTAGCAC AAGCTGCAGC
    CGGTTTGTTA CACTTGCAAA CAAGGATCAG AATCTTTCTG GAAGAACCAA GCTGGACAGA
    GAGAGGCTGA AGTCCTGTAT GAGAGAAATG GAGAGTATGG GTCAGCAGGC GAAGACCATC
    CGCACACAAG TGAAAAGAAA CACAGTTCGA GATAAACTCA AGCTAGTGTT GAATTTCCTT
    CAGAGGCTTC GATTCTTAGC AGACGAGCCC CAGCACAGCA TCCCTGATGT GTTCATATGG
    ATGATTAGCA ACAACAAACG CATCGCATAC GCCCGCATAC CCTCCAAAGA CATCCTCTAC
    TCAATTGTCG ATGAAGAGAT GGGCAAAGAC TGTGGAAAAG TTAAAGCTGT TTTTCTCAGG
    CTGCCTGGAA AGAAAGGTTT TGGGCCTGCT GGCTGGACAG TTCAGGCTAA ATTAGAGATG
    TATTTGTGGC TGGGCCTGAA TAAACAGAGG AAAGACTTCC TGATTGGCCT CCCTAGTGGC
    TTTGAAGAAA ACAAGGCAGT AAAGGGAATT GGCATACAAG CTGTCCCTCC CATCAGCCTA
    GTTTATAACA TGAAGCAGGT GTTTCAGCTG AGGGCCCACA TGTATCAAGC TCGCAGTCTG
    TTTGCTGCGG ACACTAGTGG CCTGTCTGAT CCATTTGCAA GGGTTTTCTT CTCCACCCAC
    AGCCAGGTCA CGGAGGTCCT CAGTGAGACT CTCTGTCCAA CATGGGATCA GCTGCTTGTG
    TTTGATAATG TTGAACTTTA CGGTGAGGCC GGTGAACTCC GTGATGACCC TCCAATCATT
    GTCATTGAGT TGTATGATCA AGACACTGTG GGGAAAGCTG AGTTTATTGG CCGAACATTT
    GCAAAACCAC TTACTAAGAT GGTTGATGAA CACTACGGCC CACCGCGGTT TCCTCCCCAG
    CTCGAGTATT ATCAGATCTA CCGAGGAAAC TGTGCTGCGG GAGATCTATT GGCTGCTTTT
    GAGCTTCTAC AGATTCCTTA TGACGATGAG GAGATCAGAA GAGCTCTTAT TGCTGTCCAT
    GACTTTGCTG TGCCTCAGAT CAAGATTGGT CCAGCAGGGC GGGCAGCACT TCCTCCAATC
    GATGGACCGA CCGATTCTGA CCGTGGGCCC ATTCTGCCTG TTCCATTGGG AATTCGACCA
    GTTCTCAGCA GATATCGCAT CGAGGTCCTG TTTTGGGGTC TGAGGGACCT TAAAAGGGTC
    AACCTGGCTC AGGTGGACCG ACCTCGTGTG GACATTGAGT GTGCAGGGAA AGGAGTTCAG
    TCAGCCCTAA TTCAAAACTA CAAGAAGAAT CCAAACTTCA GCACATTAGT AAAGTGGTTT
    GAAGTGGATC TGCCAGAAAA TGAGCTGCTC CATCCACCAC TTAATATTCG GGTAGTGGAT
    TGCAGGGCAT TCGGACGCTA CATTTTGGTG GGGTCTCATG CTGTGACCAC CCTTCGCAAG
    TTCATCTACA GCCCCCCAGA CAAGACTGCT AACAACTGGG CTCACACAGC TAGACTGGCC
    AATGGCTACC TGGCTCTCAG CAATGGGACG TCTCATTCCC GCCCCCAATC CCGTCCTGTT
    TCTTATCCCT CAGGTGACAT TGTGGTCAAC ATGAGCCCTG AACCTAACAT TAAGAAGATG
    GACACGGTTG TCAAGATTGA AGCTACCACC GATGCGGTTG TTAAAGTTGA CTTGAATGAG
    GATGAGAAGG AGAAAGAAAA GAAGAAGAAG AAGAAGAAGA AAGGAGAAGA AGTGGAGGAA
    GAGGAGCCGG ATGAGAGCAT GCTGGACTGG TGGTCCAAAT ACTTTGCCTC AATAGAGACT
    ATGATGGAGA ATCTAAGAGC TCAGGAGGCC GCTCAGGCAG AGGCAGAGGA AAGAGAAGAT
    TTGGAGATTG CAGCAGAGAG TGCAGAGATC AAAGCTGATG ACTTTCCTAT GAAAGGCACC
    AAACCTAAAG AGAAGAGCAA AGACAAGAAG AGCACCAAGG ACAAAAAGAA GAACAACGAT
    GGCACAGAAA AACGACCTCC AAAACCAAAA GTTGATGAAC TCATGGTGTA CAATAAAGAG
    CTGGAGAGTG AGTTCGGTAG CTTTGAGGAC TGGCTTCACA CCTTCAACCT GTACCGAGGA
    AAGGCTGGAG ATGATGATGA CCACAATGTG GTTGATGAGG ACAGAATTGT GGGCAGATTC
    AAGGGCTCCC TCTGTATGTA TAAACTACCA CTGTCTGAGG AGATCACCAG AGAGGCAGGA
    TTTGATCCAA ACATGGGCAT GTTCCAGAGT ATTCCTCACA ATGACCCTAT CAACGTTCTT
    GTCAGGATTT ATATCATTCG AGCCACAGAC CTGCATCCTG CAGATATAAA TGGAAAGGCC
    GACCCTTATA TAGTCATTAA ATTGGGAAAG TCAGACATTC GAGACAAGGA GAACTACATA
    TCCAAGCAGC TGAATCCTGT CTTTGGAAAG TCATTTGATA TAGAGGCCAC GTTCCCAATG
    GAGTCTATGC TGACTGTGGC AGTGTATGAC TGGGATTTAG TCGGAACTGA TGACCTGATT
    GGTGAGACTA AGATTGATTT GGAGAACAGA TACTACAGCA AACACAGAGC CACATGTGGC
    ATTGCTTCCA ACTACTCTGT CCATGGTTAT AATGTATGGA GAGATCCTCA GAAACCAGCT
    CAGATCCTGG CTAAGTTGTG CAAAGAAGGT AAACTGGATG GACCTCACTA TGGACCTGGG
    GGAAGAGTCA AAGTTGCAAA CCGGATCTTT CTTGGGCCGA CAGAAATTGA GGATGAGAGT
    GGTCTGAAAA AACAAACAGA AGAACACTTG GCGCTCACAG TCCTTAGACA TTGGGAGGAA
    ATCCCACGTG TTGGTTGCAA ACTCATCCCT GAGCATGTGG AGACGAGGCC TCTCCTCAAC
    CCAGACAAGC CAGGCATAGA GCAGGGAAGG ATTGAAATGT GGGTGGACAT GTTTCCTATG
    GATGTCCCAG CACCAGGACC AGCCATTGAT ATATCGCCAC GCAAACCAAA GAGCTTTGAG
    CTTCGTGTTA TTATTTGGAA CACTGATGAC GTAATTCTAG AGGACGATGC TTTCATGACA
    GGAGAGAAGA TGTCAGACAT CTATGTCAGG GGCTGGCTTA AAGGACAGCA GGAGGACAAA
    CAGGACACCG ATGTACATTA TCACTCTCTA ACTGGAGAGG GAAACTTCAA CTGGCGCTTT
    GTCTTCCCCT TTGACTATCT CATGGCCGAG GAGAAGATTG TTATCTCCAA GAAGGAATCA
    ATGTTTTCCT GGGATGAGAC TGAGTATAAG ATCCCTGCTC GACTCACCCT TCAAGTATGG
    GATGCCGATC ACTTTTCTGC AGATGATTTT CTGGGTGCGA TTGAGTTGGA CTTGAATAAG
    TTTCCGCGTG GGGCAAAAAC AGCCAAGCAG TGCTCTCTAG ACATGGTTCT CAAAGAGCAT
    GAGCTGCCAA CCATCTCCAT CTTCAAACAA AAGAGAGTCA AGGGCTGGTG GCCATTTGTG
    GCCCAAAATG AGAATGATGA ATTTGAGCTA ACGGGAAAAG TAGAGGCAGA GCTCCATCTG
    TTGACAGCAG AGGAGGCAGA GAAGAGTCCA GTCGGCCTGG GGAGGAATGA TCCGGAGCCA
    CTTGAGAAAC CAAATCGTCC AACTACCAGC CTGTCGTGGC TGTTGAGTCC GATTCGGGTG
    ACCAGCATAA TGTGTTGGCG GCAGTATAAG TTGGTCACAG TGGTGACTCT GCTCATCATA
    ACCGGGCTGA TCTTTTTGGG CCTCCTGTTC TTTTCTCTGC CTTCAGCTTT CATGCAGAAG
    CTCTTCAACA TCTCTGGATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009293008.1
    CDS303..6443
    Translation

    Target ORF information:

    RefSeq Version XM_009294733.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio otoferlin a (otofa), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009294733.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCTTTAG TGGTGCATCT GAAGACTGTC ACGGAGCTCC GTGGGAAGGG GGACCGAATC 
    GCTAAAGTCA CTTTCAGAGG TCTCTCCTTC TTCTCTCGGG TGTTGGAAAA CTGCGAGGAT
    GAAGCTCGAT TTGAGCAGGC GTTCCGATGG CCAATTGGGA GTCAAGTGGA TGGCGACGAG
    ATGCTGGAGA TCCAAGTGTT TAATTACAGC AAAGTCTTCA CTAATAGGTT AATCGGCACA
    TTCCGCATGG TTCTTCAGAA GGTGGTGGAG GAGGGACACC TGGAGGTGTC TGACACACTC
    ATTGACGACA ATAACACAGC CATACAGACA ACCATTTCCA TAGAGATCAA ATATCAGACC
    ATGGACGGGA GTGTAAAGGT GTGGAGCGAT GGTGAATTCC TTGATATTCC AGATGACCTT
    GATGGAACCT TCCAGTTTGA GACAGATAGT CTTCTCTCAG GACGCAGCCA GAGTTCTGGA
    ACTTCACCTG GACGATCCAT TCATGGCATT CCCACATTCC GCAAGGCAGG AAAAGGAGTG
    TTCTCAGCCA TGAAGCTGGG CAAGACCCGC ACCTCTAAAG ATGACCACAA AAAAGGAGAT
    GATGCTGCCA TTCTGGATGC AGAAGATCTG GATCGAAAAG CCATGCGTCT AGGCGGCATT
    CTTGACCCTG ATACAATCTC ACTGGCCTCG GTTACTGCAG TGACCACTAA TGTCTCCAAC
    AAAAGATCAA AGCCTGACAT TAAAATGGAG CCGAGTTCAG GGCGTCCGGT TGATTATCAG
    ATCAGTGTTA CAGTAATCGA GGCCCGTCAG CTGGTCGGAT TGAACATGGA CCCTGTGGTT
    TGCGTGGAAA TTGGAGAGGA GAAGAAGTAC ACGTCAATGA AGGAGTCAAC AAACTGCCCT
    TACTACAATG AGTATTTTGT CTTCGACTTC CACGTGCCTC CAGACGTCAT GTTTGACAAA
    ATTATAAAGA TTTCAGTAAT ACATTCTAAA AACCTTCTGC GAAGCGGCAC CCTCGTGGGC
    ACCTTCAAAC TAGACGTGGG GACTGTTTAC ACACAACCTG AACATCAGTT TCATCACAAA
    TGGGCGATGC TGTCAGACCC TGATGACATC ACGACTGGCT GCAAAGGTTA TGTTAAATGT
    GACATCGCAG TCGTGGGAAA AGGAGACAAC ATCAAGACTC CACACAAGGC CAGTGAAGCA
    GACGAGGATG ACATAGAGGG GAATCTTCTC TTGCCGGAGG GTGTTCCATC AGAGAGACAG
    TGGGCTCGGT TTTATGTTAA GATTTATAGA GCTGAGGGTC TGCCTAAAAT GAACACCAGT
    ATCATGGCCA ACGTGAAGAA AGCCTTTATT GGGGAAAACA GGGACCTTGT GGACCCTTAC
    GTCCTGGTGC AATTTGCAGG GCAAAAGGGC AAAACATCTG TTCAGAAGAG CAGCTACGAG
    CCCATCTGGA ACGAGCAAGT CATCTTCACG GAGATGTTCC CACCTTTGTG TAGGCGACTG
    AAAGTTCAGA TCCGTGATTC AGACAAGGTG AACGATGTTG CCATAGGAAC CCACTTCATT
    GATCTACGAA AGGTTTCCAA TGATGGAGAC AAAGGTTTCT TGCCCACTAT GGGTCCGGCT
    TGGGTGAATA TGTATGGGTC GACCCGTAAC TACACCCTGA TGGACGAACA CCAGGACCTG
    AATGAGGGGT TGGGGGAAGG GGTGTCCTTC AGAGCACGTC TGCTCATTAG TATCGCGGTG
    GAGATTCTAG ACACCACCTC TGCTGAGATC ATGAGCTCCA CTGAAGTACA CATGGAGCCG
    GTGTCCAACA TCTCAGAGAG TGCTACAGGA AAGATGGAAG AATTTTTCCT TTTCGGGTCA
    TTCTTGGAGG CCACAATGAT AGACAGAAAA ATAGGAGATA AACCCATCAG CTTTGAAGTC
    ACCATCGGTA ACTATGGTAA CCAGATTGAT GGTGTGAGCA AGCCTGCATT AGCGAAGAAG
    AAGAAGGAGG GTGGAGGAGA GAGTGAAGAA GAGGAGTCTG AGCTTATCCA CAACTCCAGC
    GAGGAAGAGG CAGAGGATGA TGGAGATCTG ACCTCTGTAC CGTCTACACC TCCTATGAAG
    CCTGTTATCA CCGACAGAAA TTACTTCCAC TTGCCTTACT TTGAAAAGAA ACCCTGCATT
    TACATCAAGA GCTGGTGGCA GGACCAGCGG AGACGTCTCT ACAACTCCAA CATAATGGAC
    AAGATAGCTG ATAAACTGGA GGAAGGTCTG AATGATGTTC AAGAGATCAT AAAAACAGAG
    AAGGCATATC CAGAACGAAG ACTAAGAGGT GTATTAGAAG AACTTAGCAC AAGCTGCAGC
    CGGTTTGTTA CACTTGCAAA CAAGGATCAG AATCTTTCTG GAAGAACCAA GCTGGACAGA
    GAGAGGCTGA AGTCCTGTAT GAGAGAAATG GAGAGTATGG GTCAGCAGGC GAAGACCATC
    CGCACACAAG TGAAAAGAAA CACAGTTCGA GATAAACTCA AGCTAGTGTT GAATTTCCTT
    CAGAGGCTTC GATTCTTAGC AGACGAGCCC CAGCACAGCA TCCCTGATGT GTTCATATGG
    ATGATTAGCA ACAACAAACG CATCGCATAC GCCCGCATAC CCTCCAAAGA CATCCTCTAC
    TCAATTGTCG ATGAAGAGAT GGGCAAAGAC TGTGGAAAAG TTAAAGCTGT TTTTCTCAGG
    CTGCCTGGAA AGAAAGGTTT TGGGCCTGCT GGCTGGACAG TTCAGGCTAA ATTAGAGATG
    TATTTGTGGC TGGGCCTGAA TAAACAGAGG AAAGACTTCC TGATTGGCCT CCCTAGTGGC
    TTTGAAGAAA ACAAGGCAGT AAAGGGAATT GGCATACAAG CTGTCCCTCC CATCAGCCTA
    GTTTATAACA TGAAGCAGGT GTTTCAGCTG AGGGCCCACA TGTATCAAGC TCGCAGTCTG
    TTTGCTGCGG ACACTAGTGG CCTGTCTGAT CCATTTGCAA GGGTTTTCTT CTCCACCCAC
    AGCCAGGTCA CGGAGGTCCT CAGTGAGACT CTCTGTCCAA CATGGGATCA GCTGCTTGTG
    TTTGATAATG TTGAACTTTA CGGTGAGGCC GGTGAACTCC GTGATGACCC TCCAATCATT
    GTCATTGAGT TGTATGATCA AGACACTGTG GGGAAAGCTG AGTTTATTGG CCGAACATTT
    GCAAAACCAC TTACTAAGAT GGTTGATGAA CACTACGGCC CACCGCGGTT TCCTCCCCAG
    CTCGAGTATT ATCAGATCTA CCGAGGAAAC TGTGCTGCGG GAGATCTATT GGCTGCTTTT
    GAGCTTCTAC AGATTCCTTA TGACGATGAG GAGATCAGAA GAGCTCTTAT TGCTGTCCAT
    GACTTTGCTG TGCCTCAGAT CAAGATTGGT CCAGCAGGGC GGGCAGCACT TCCTCCAATC
    GATGGACCGA CCGATTCTGA CCGTGGGCCC ATTCTGCCTG TTCCATTGGG AATTCGACCA
    GTTCTCAGCA GATATCGCAT CGAGGTCCTG TTTTGGGGTC TGAGGGACCT TAAAAGGGTC
    AACCTGGCTC AGGTGGACCG ACCTCGTGTG GACATTGAGT GTGCAGGGAA AGGAGTTCAG
    TCAGCCCTAA TTCAAAACTA CAAGAAGAAT CCAAACTTCA GCACATTAGT AAAGTGGTTT
    GAAGTGGATC TGCCAGAAAA TGAGCTGCTC CATCCACCAC TTAATATTCG GGTAGTGGAT
    TGCAGGGCAT TCGGACGCTA CATTTTGGTG GGGTCTCATG CTGTGACCAC CCTTCGCAAG
    TTCATCTACA GCCCCCCAGA CAAGACTGCT AACAACTGGG CTCACACAGC TAGACTGGCC
    AATGGCTACC TGGCTCTCAG CAATGGGACG TCTCATTCCC GCCCCCAATC CCGTCCTGTT
    TCTTATCCCT CAGGTGACAT TGTGGTCAAC ATGAGCCCTG AACCTAACAT TAAGAAGATG
    GACACGGTTG TCAAGATTGA AGCTACCACC GATGCGGTTG TTAAAGTTGA CTTGAATGAG
    GATGAGAAGG AGAAAGAAAA GAAGAAGAAG AAGAAGAAGA AAGGAGAAGA AGTGGAGGAA
    GAGGAGCCGG ATGAGAGCAT GCTGGACTGG TGGTCCAAAT ACTTTGCCTC AATAGAGACT
    ATGATGGAGA ATCTAAGAGC TCAGGAGGCC GCTCAGGCAG AGGCAGAGGA AAGAGAAGAT
    TTGGAGATTG CAGCAGAGAG TGCAGAGATC AAAGCTGATG ACTTTCCTAT GAAAGGCACC
    AAACCTAAAG AGAAGAGCAA AGACAAGAAG AGCACCAAGG ACAAAAAGAA GAACAACGAT
    GGCACAGAAA AACGACCTCC AAAACCAAAA GTTGATGAAC TCATGGTGTA CAATAAAGAG
    CTGGAGAGTG AGTTCGGTAG CTTTGAGGAC TGGCTTCACA CCTTCAACCT GTACCGAGGA
    AAGGCTGGAG ATGATGATGA CCACAATGTG GTTGATGAGG ACAGAATTGT GGGCAGATTC
    AAGGGCTCCC TCTGTATGTA TAAACTACCA CTGTCTGAGG AGATCACCAG AGAGGCAGGA
    TTTGATCCAA ACATGGGCAT GTTCCAGAGT ATTCCTCACA ATGACCCTAT CAACGTTCTT
    GTCAGGATTT ATATCATTCG AGCCACAGAC CTGCATCCTG CAGATATAAA TGGAAAGGCC
    GACCCTTATA TAGTCATTAA ATTGGGAAAG TCAGACATTC GAGACAAGGA GAACTACATA
    TCCAAGCAGC TGAATCCTGT CTTTGGAAAG TCATTTGATA TAGAGGCCAC GTTCCCAATG
    GAGTCTATGC TGACTGTGGC AGTGTATGAC TGGGATTTAG TCGGAACTGA TGACCTGATT
    GGTGAGACTA AGATTGATTT GGAGAACAGA TACTACAGCA AACACAGAGC CACATGTGGC
    ATTGCTTCCA ACTACTCTGT CCATGGTTAT AATGTATGGA GAGATCCTCA GAAACCAGCT
    CAGATCCTGG CTAAGTTGTG CAAAGAAGGT AAACTGGATG GACCTCACTA TGGACCTGGG
    GGAAGAGTCA AAGTTGCAAA CCGGATCTTT CTTGGGCCGA CAGAAATTGA GGATGAGAGT
    GGTCTGAAAA AACAAACAGA AGAACACTTG GCGCTCACAG TCCTTAGACA TTGGGAGGAA
    ATCCCACGTG TTGGTTGCAA ACTCATCCCT GAGCATGTGG AGACGAGGCC TCTCCTCAAC
    CCAGACAAGC CAGGCATAGA GCAGGGAAGG ATTGAAATGT GGGTGGACAT GTTTCCTATG
    GATGTCCCAG CACCAGGACC AGCCATTGAT ATATCGCCAC GCAAACCAAA GAGCTTTGAG
    CTTCGTGTTA TTATTTGGAA CACTGATGAC GTAATTCTAG AGGACGATGC TTTCATGACA
    GGAGAGAAGA TGTCAGACAT CTATGTCAGG GGCTGGCTTA AAGGACAGCA GGAGGACAAA
    CAGGACACCG ATGTACATTA TCACTCTCTA ACTGGAGAGG GAAACTTCAA CTGGCGCTTT
    GTCTTCCCCT TTGACTATCT CATGGCCGAG GAGAAGATTG TTATCTCCAA GAAGGAATCA
    ATGTTTTCCT GGGATGAGAC TGAGTATAAG ATCCCTGCTC GACTCACCCT TCAAGTATGG
    GATGCCGATC ACTTTTCTGC AGATGATTTT CTGGGTGCGA TTGAGTTGGA CTTGAATAAG
    TTTCCGCGTG GGGCAAAAAC AGCCAAGCAG TGCTCTCTAG ACATGGTTCT CAAAGAGCAT
    GAGCTGCCAA CCATCTCCAT CTTCAAACAA AAGAGAGTCA AGGGCTGGTG GCCATTTGTG
    GCCCAAAATG AGAATGATGA ATTTGAGCTA ACGGGAAAAG TAGAGGCAGA GCTCCATCTG
    TTGACAGCAG AGGAGGCAGA GAAGAGTCCA GTCGGCCTGG GGAGGAATGA TCCGGAGCCA
    CTTGAGAAAC CAAATCGTCC AACTACCAGC CTGTCGTGGC TGTTGAGTCC GATTCGGGTG
    ACCAGCATAA TGTGTTGGCG GCAGTATAAG TTGGTCACAG TGGTGACTCT GCTCATCATA
    ACCGGGCTGA TCTTTTTGGG CCTCCTGTTC TTTTCTCTGC CTTCAGCTTT CATGCAGAAG
    CTCTTCAACA TCTCTGGATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42605
    Clone ID Related Accession (Same CDS sequence) XM_009294733.2 , XM_009294733.3
    Accession Version XM_009294733.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6141bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product otoferlin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009294733.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCTTTAG TGGTGCATCT GAAGACTGTC ACGGAGCTCC GTGGGAAGGG GGACCGAATC 
    GCTAAAGTCA CTTTCAGAGG TCTCTCCTTC TTCTCTCGGG TGTTGGAAAA CTGCGAGGAT
    GAAGCTCGAT TTGAGCAGGC GTTCCGATGG CCAATTGGGA GTCAAGTGGA TGGCGACGAG
    ATGCTGGAGA TCCAAGTGTT TAATTACAGC AAAGTCTTCA CTAATAGGTT AATCGGCACA
    TTCCGCATGG TTCTTCAGAA GGTGGTGGAG GAGGGACACC TGGAGGTGTC TGACACACTC
    ATTGACGACA ATAACACAGC CATACAGACA ACCATTTCCA TAGAGATCAA ATATCAGACC
    ATGGACGGGA GTGTAAAGGT GTGGAGCGAT GGTGAATTCC TTGATATTCC AGATGACCTT
    GATGGAACCT TCCAGTTTGA GACAGATAGT CTTCTCTCAG GACGCAGCCA GAGTTCTGGA
    ACTTCACCTG GACGATCCAT TCATGGCATT CCCACATTCC GCAAGGCAGG AAAAGGAGTG
    TTCTCAGCCA TGAAGCTGGG CAAGACCCGC ACCTCTAAAG ATGACCACAA AAAAGGAGAT
    GATGCTGCCA TTCTGGATGC AGAAGATCTG GATCGAAAAG CCATGCGTCT AGGCGGCATT
    CTTGACCCTG ATACAATCTC ACTGGCCTCG GTTACTGCAG TGACCACTAA TGTCTCCAAC
    AAAAGATCAA AGCCTGACAT TAAAATGGAG CCGAGTTCAG GGCGTCCGGT TGATTATCAG
    ATCAGTGTTA CAGTAATCGA GGCCCGTCAG CTGGTCGGAT TGAACATGGA CCCTGTGGTT
    TGCGTGGAAA TTGGAGAGGA GAAGAAGTAC ACGTCAATGA AGGAGTCAAC AAACTGCCCT
    TACTACAATG AGTATTTTGT CTTCGACTTC CACGTGCCTC CAGACGTCAT GTTTGACAAA
    ATTATAAAGA TTTCAGTAAT ACATTCTAAA AACCTTCTGC GAAGCGGCAC CCTCGTGGGC
    ACCTTCAAAC TAGACGTGGG GACTGTTTAC ACACAACCTG AACATCAGTT TCATCACAAA
    TGGGCGATGC TGTCAGACCC TGATGACATC ACGACTGGCT GCAAAGGTTA TGTTAAATGT
    GACATCGCAG TCGTGGGAAA AGGAGACAAC ATCAAGACTC CACACAAGGC CAGTGAAGCA
    GACGAGGATG ACATAGAGGG GAATCTTCTC TTGCCGGAGG GTGTTCCATC AGAGAGACAG
    TGGGCTCGGT TTTATGTTAA GATTTATAGA GCTGAGGGTC TGCCTAAAAT GAACACCAGT
    ATCATGGCCA ACGTGAAGAA AGCCTTTATT GGGGAAAACA GGGACCTTGT GGACCCTTAC
    GTCCTGGTGC AATTTGCAGG GCAAAAGGGC AAAACATCTG TTCAGAAGAG CAGCTACGAG
    CCCATCTGGA ACGAGCAAGT CATCTTCACG GAGATGTTCC CACCTTTGTG TAGGCGACTG
    AAAGTTCAGA TCCGTGATTC AGACAAGGTG AACGATGTTG CCATAGGAAC CCACTTCATT
    GATCTACGAA AGGTTTCCAA TGATGGAGAC AAAGGTTTCT TGCCCACTAT GGGTCCGGCT
    TGGGTGAATA TGTATGGGTC GACCCGTAAC TACACCCTGA TGGACGAACA CCAGGACCTG
    AATGAGGGGT TGGGGGAAGG GGTGTCCTTC AGAGCACGTC TGCTCATTAG TATCGCGGTG
    GAGATTCTAG ACACCACCTC TGCTGAGATC ATGAGCTCCA CTGAAGTACA CATGGAGCCG
    GTGTCCAACA TCTCAGAGAG TGCTACAGGA AAGATGGAAG AATTTTTCCT TTTCGGGTCA
    TTCTTGGAGG CCACAATGAT AGACAGAAAA ATAGGAGATA AACCCATCAG CTTTGAAGTC
    ACCATCGGTA ACTATGGTAA CCAGATTGAT GGTGTGAGCA AGCCTGCATT AGCGAAGAAG
    AAGAAGGAGG GTGGAGGAGA GAGTGAAGAA GAGGAGTCTG AGCTTATCCA CAACTCCAGC
    GAGGAAGAGG CAGAGGATGA TGGAGATCTG ACCTCTGTAC CGTCTACACC TCCTATGAAG
    CCTGTTATCA CCGACAGAAA TTACTTCCAC TTGCCTTACT TTGAAAAGAA ACCCTGCATT
    TACATCAAGA GCTGGTGGCA GGACCAGCGG AGACGTCTCT ACAACTCCAA CATAATGGAC
    AAGATAGCTG ATAAACTGGA GGAAGGTCTG AATGATGTTC AAGAGATCAT AAAAACAGAG
    AAGGCATATC CAGAACGAAG ACTAAGAGGT GTATTAGAAG AACTTAGCAC AAGCTGCAGC
    CGGTTTGTTA CACTTGCAAA CAAGGATCAG AATCTTTCTG GAAGAACCAA GCTGGACAGA
    GAGAGGCTGA AGTCCTGTAT GAGAGAAATG GAGAGTATGG GTCAGCAGGC GAAGACCATC
    CGCACACAAG TGAAAAGAAA CACAGTTCGA GATAAACTCA AGCTAGTGTT GAATTTCCTT
    CAGAGGCTTC GATTCTTAGC AGACGAGCCC CAGCACAGCA TCCCTGATGT GTTCATATGG
    ATGATTAGCA ACAACAAACG CATCGCATAC GCCCGCATAC CCTCCAAAGA CATCCTCTAC
    TCAATTGTCG ATGAAGAGAT GGGCAAAGAC TGTGGAAAAG TTAAAGCTGT TTTTCTCAGG
    CTGCCTGGAA AGAAAGGTTT TGGGCCTGCT GGCTGGACAG TTCAGGCTAA ATTAGAGATG
    TATTTGTGGC TGGGCCTGAA TAAACAGAGG AAAGACTTCC TGATTGGCCT CCCTAGTGGC
    TTTGAAGAAA ACAAGGCAGT AAAGGGAATT GGCATACAAG CTGTCCCTCC CATCAGCCTA
    GTTTATAACA TGAAGCAGGT GTTTCAGCTG AGGGCCCACA TGTATCAAGC TCGCAGTCTG
    TTTGCTGCGG ACACTAGTGG CCTGTCTGAT CCATTTGCAA GGGTTTTCTT CTCCACCCAC
    AGCCAGGTCA CGGAGGTCCT CAGTGAGACT CTCTGTCCAA CATGGGATCA GCTGCTTGTG
    TTTGATAATG TTGAACTTTA CGGTGAGGCC GGTGAACTCC GTGATGACCC TCCAATCATT
    GTCATTGAGT TGTATGATCA AGACACTGTG GGGAAAGCTG AGTTTATTGG CCGAACATTT
    GCAAAACCAC TTACTAAGAT GGTTGATGAA CACTACGGCC CACCGCGGTT TCCTCCCCAG
    CTCGAGTATT ATCAGATCTA CCGAGGAAAC TGTGCTGCGG GAGATCTATT GGCTGCTTTT
    GAGCTTCTAC AGATTCCTTA TGACGATGAG GAGATCAGAA GAGCTCTTAT TGCTGTCCAT
    GACTTTGCTG TGCCTCAGAT CAAGATTGGT CCAGCAGGGC GGGCAGCACT TCCTCCAATC
    GATGGACCGA CCGATTCTGA CCGTGGGCCC ATTCTGCCTG TTCCATTGGG AATTCGACCA
    GTTCTCAGCA GATATCGCAT CGAGGTCCTG TTTTGGGGTC TGAGGGACCT TAAAAGGGTC
    AACCTGGCTC AGGTGGACCG ACCTCGTGTG GACATTGAGT GTGCAGGGAA AGGAGTTCAG
    TCAGCCCTAA TTCAAAACTA CAAGAAGAAT CCAAACTTCA GCACATTAGT AAAGTGGTTT
    GAAGTGGATC TGCCAGAAAA TGAGCTGCTC CATCCACCAC TTAATATTCG GGTAGTGGAT
    TGCAGGGCAT TCGGACGCTA CATTTTGGTG GGGTCTCATG CTGTGACCAC CCTTCGCAAG
    TTCATCTACA GCCCCCCAGA CAAGACTGCT AACAACTGGG CTCACACAGC TAGACTGGCC
    AATGGCTACC TGGCTCTCAG CAATGGGACG TCTCATTCCC GCCCCCAATC CCGTCCTGTT
    TCTTATCCCT CAGGTGACAT TGTGGTCAAC ATGAGCCCTG AACCTAACAT TAAGAAGATG
    GACACGGTTG TCAAGATTGA AGCTACCACC GATGCGGTTG TTAAAGTTGA CTTGAATGAG
    GATGAGAAGG AGAAAGAAAA GAAGAAGAAG AAGAAGAAGA AAGGAGAAGA AGTGGAGGAA
    GAGGAGCCGG ATGAGAGCAT GCTGGACTGG TGGTCCAAAT ACTTTGCCTC AATAGAGACT
    ATGATGGAGA ATCTAAGAGC TCAGGAGGCC GCTCAGGCAG AGGCAGAGGA AAGAGAAGAT
    TTGGAGATTG CAGCAGAGAG TGCAGAGATC AAAGCTGATG ACTTTCCTAT GAAAGGCACC
    AAACCTAAAG AGAAGAGCAA AGACAAGAAG AGCACCAAGG ACAAAAAGAA GAACAACGAT
    GGCACAGAAA AACGACCTCC AAAACCAAAA GTTGATGAAC TCATGGTGTA CAATAAAGAG
    CTGGAGAGTG AGTTCGGTAG CTTTGAGGAC TGGCTTCACA CCTTCAACCT GTACCGAGGA
    AAGGCTGGAG ATGATGATGA CCACAATGTG GTTGATGAGG ACAGAATTGT GGGCAGATTC
    AAGGGCTCCC TCTGTATGTA TAAACTACCA CTGTCTGAGG AGATCACCAG AGAGGCAGGA
    TTTGATCCAA ACATGGGCAT GTTCCAGAGT ATTCCTCACA ATGACCCTAT CAACGTTCTT
    GTCAGGATTT ATATCATTCG AGCCACAGAC CTGCATCCTG CAGATATAAA TGGAAAGGCC
    GACCCTTATA TAGTCATTAA ATTGGGAAAG TCAGACATTC GAGACAAGGA GAACTACATA
    TCCAAGCAGC TGAATCCTGT CTTTGGAAAG TCATTTGATA TAGAGGCCAC GTTCCCAATG
    GAGTCTATGC TGACTGTGGC AGTGTATGAC TGGGATTTAG TCGGAACTGA TGACCTGATT
    GGTGAGACTA AGATTGATTT GGAGAACAGA TACTACAGCA AACACAGAGC CACATGTGGC
    ATTGCTTCCA ACTACTCTGT CCATGGTTAT AATGTATGGA GAGATCCTCA GAAACCAGCT
    CAGATCCTGG CTAAGTTGTG CAAAGAAGGT AAACTGGATG GACCTCACTA TGGACCTGGG
    GGAAGAGTCA AAGTTGCAAA CCGGATCTTT CTTGGGCCGA CAGAAATTGA GGATGAGAGT
    GGTCTGAAAA AACAAACAGA AGAACACTTG GCGCTCACAG TCCTTAGACA TTGGGAGGAA
    ATCCCACGTG TTGGTTGCAA ACTCATCCCT GAGCATGTGG AGACGAGGCC TCTCCTCAAC
    CCAGACAAGC CAGGCATAGA GCAGGGAAGG ATTGAAATGT GGGTGGACAT GTTTCCTATG
    GATGTCCCAG CACCAGGACC AGCCATTGAT ATATCGCCAC GCAAACCAAA GAGCTTTGAG
    CTTCGTGTTA TTATTTGGAA CACTGATGAC GTAATTCTAG AGGACGATGC TTTCATGACA
    GGAGAGAAGA TGTCAGACAT CTATGTCAGG GGCTGGCTTA AAGGACAGCA GGAGGACAAA
    CAGGACACCG ATGTACATTA TCACTCTCTA ACTGGAGAGG GAAACTTCAA CTGGCGCTTT
    GTCTTCCCCT TTGACTATCT CATGGCCGAG GAGAAGATTG TTATCTCCAA GAAGGAATCA
    ATGTTTTCCT GGGATGAGAC TGAGTATAAG ATCCCTGCTC GACTCACCCT TCAAGTATGG
    GATGCCGATC ACTTTTCTGC AGATGATTTT CTGGGTGCGA TTGAGTTGGA CTTGAATAAG
    TTTCCGCGTG GGGCAAAAAC AGCCAAGCAG TGCTCTCTAG ACATGGTTCT CAAAGAGCAT
    GAGCTGCCAA CCATCTCCAT CTTCAAACAA AAGAGAGTCA AGGGCTGGTG GCCATTTGTG
    GCCCAAAATG AGAATGATGA ATTTGAGCTA ACGGGAAAAG TAGAGGCAGA GCTCCATCTG
    TTGACAGCAG AGGAGGCAGA GAAGAGTCCA GTCGGCCTGG GGAGGAATGA TCCGGAGCCA
    CTTGAGAAAC CAAATCGTCC AACTACCAGC CTGTCGTGGC TGTTGAGTCC GATTCGGGTG
    ACCAGCATAA TGTGTTGGCG GCAGTATAAG TTGGTCACAG TGGTGACTCT GCTCATCATA
    ACCGGGCTGA TCTTTTTGGG CCTCCTGTTC TTTTCTCTGC CTTCAGCTTT CATGCAGAAG
    CTCTTCAACA TCTCTGGATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009293008.1
    CDS301..6441
    Translation

    Target ORF information:

    RefSeq Version XM_009294733.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio otoferlin a (otofa), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009294733.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCTTTAG TGGTGCATCT GAAGACTGTC ACGGAGCTCC GTGGGAAGGG GGACCGAATC 
    GCTAAAGTCA CTTTCAGAGG TCTCTCCTTC TTCTCTCGGG TGTTGGAAAA CTGCGAGGAT
    GAAGCTCGAT TTGAGCAGGC GTTCCGATGG CCAATTGGGA GTCAAGTGGA TGGCGACGAG
    ATGCTGGAGA TCCAAGTGTT TAATTACAGC AAAGTCTTCA CTAATAGGTT AATCGGCACA
    TTCCGCATGG TTCTTCAGAA GGTGGTGGAG GAGGGACACC TGGAGGTGTC TGACACACTC
    ATTGACGACA ATAACACAGC CATACAGACA ACCATTTCCA TAGAGATCAA ATATCAGACC
    ATGGACGGGA GTGTAAAGGT GTGGAGCGAT GGTGAATTCC TTGATATTCC AGATGACCTT
    GATGGAACCT TCCAGTTTGA GACAGATAGT CTTCTCTCAG GACGCAGCCA GAGTTCTGGA
    ACTTCACCTG GACGATCCAT TCATGGCATT CCCACATTCC GCAAGGCAGG AAAAGGAGTG
    TTCTCAGCCA TGAAGCTGGG CAAGACCCGC ACCTCTAAAG ATGACCACAA AAAAGGAGAT
    GATGCTGCCA TTCTGGATGC AGAAGATCTG GATCGAAAAG CCATGCGTCT AGGCGGCATT
    CTTGACCCTG ATACAATCTC ACTGGCCTCG GTTACTGCAG TGACCACTAA TGTCTCCAAC
    AAAAGATCAA AGCCTGACAT TAAAATGGAG CCGAGTTCAG GGCGTCCGGT TGATTATCAG
    ATCAGTGTTA CAGTAATCGA GGCCCGTCAG CTGGTCGGAT TGAACATGGA CCCTGTGGTT
    TGCGTGGAAA TTGGAGAGGA GAAGAAGTAC ACGTCAATGA AGGAGTCAAC AAACTGCCCT
    TACTACAATG AGTATTTTGT CTTCGACTTC CACGTGCCTC CAGACGTCAT GTTTGACAAA
    ATTATAAAGA TTTCAGTAAT ACATTCTAAA AACCTTCTGC GAAGCGGCAC CCTCGTGGGC
    ACCTTCAAAC TAGACGTGGG GACTGTTTAC ACACAACCTG AACATCAGTT TCATCACAAA
    TGGGCGATGC TGTCAGACCC TGATGACATC ACGACTGGCT GCAAAGGTTA TGTTAAATGT
    GACATCGCAG TCGTGGGAAA AGGAGACAAC ATCAAGACTC CACACAAGGC CAGTGAAGCA
    GACGAGGATG ACATAGAGGG GAATCTTCTC TTGCCGGAGG GTGTTCCATC AGAGAGACAG
    TGGGCTCGGT TTTATGTTAA GATTTATAGA GCTGAGGGTC TGCCTAAAAT GAACACCAGT
    ATCATGGCCA ACGTGAAGAA AGCCTTTATT GGGGAAAACA GGGACCTTGT GGACCCTTAC
    GTCCTGGTGC AATTTGCAGG GCAAAAGGGC AAAACATCTG TTCAGAAGAG CAGCTACGAG
    CCCATCTGGA ACGAGCAAGT CATCTTCACG GAGATGTTCC CACCTTTGTG TAGGCGACTG
    AAAGTTCAGA TCCGTGATTC AGACAAGGTG AACGATGTTG CCATAGGAAC CCACTTCATT
    GATCTACGAA AGGTTTCCAA TGATGGAGAC AAAGGTTTCT TGCCCACTAT GGGTCCGGCT
    TGGGTGAATA TGTATGGGTC GACCCGTAAC TACACCCTGA TGGACGAACA CCAGGACCTG
    AATGAGGGGT TGGGGGAAGG GGTGTCCTTC AGAGCACGTC TGCTCATTAG TATCGCGGTG
    GAGATTCTAG ACACCACCTC TGCTGAGATC ATGAGCTCCA CTGAAGTACA CATGGAGCCG
    GTGTCCAACA TCTCAGAGAG TGCTACAGGA AAGATGGAAG AATTTTTCCT TTTCGGGTCA
    TTCTTGGAGG CCACAATGAT AGACAGAAAA ATAGGAGATA AACCCATCAG CTTTGAAGTC
    ACCATCGGTA ACTATGGTAA CCAGATTGAT GGTGTGAGCA AGCCTGCATT AGCGAAGAAG
    AAGAAGGAGG GTGGAGGAGA GAGTGAAGAA GAGGAGTCTG AGCTTATCCA CAACTCCAGC
    GAGGAAGAGG CAGAGGATGA TGGAGATCTG ACCTCTGTAC CGTCTACACC TCCTATGAAG
    CCTGTTATCA CCGACAGAAA TTACTTCCAC TTGCCTTACT TTGAAAAGAA ACCCTGCATT
    TACATCAAGA GCTGGTGGCA GGACCAGCGG AGACGTCTCT ACAACTCCAA CATAATGGAC
    AAGATAGCTG ATAAACTGGA GGAAGGTCTG AATGATGTTC AAGAGATCAT AAAAACAGAG
    AAGGCATATC CAGAACGAAG ACTAAGAGGT GTATTAGAAG AACTTAGCAC AAGCTGCAGC
    CGGTTTGTTA CACTTGCAAA CAAGGATCAG AATCTTTCTG GAAGAACCAA GCTGGACAGA
    GAGAGGCTGA AGTCCTGTAT GAGAGAAATG GAGAGTATGG GTCAGCAGGC GAAGACCATC
    CGCACACAAG TGAAAAGAAA CACAGTTCGA GATAAACTCA AGCTAGTGTT GAATTTCCTT
    CAGAGGCTTC GATTCTTAGC AGACGAGCCC CAGCACAGCA TCCCTGATGT GTTCATATGG
    ATGATTAGCA ACAACAAACG CATCGCATAC GCCCGCATAC CCTCCAAAGA CATCCTCTAC
    TCAATTGTCG ATGAAGAGAT GGGCAAAGAC TGTGGAAAAG TTAAAGCTGT TTTTCTCAGG
    CTGCCTGGAA AGAAAGGTTT TGGGCCTGCT GGCTGGACAG TTCAGGCTAA ATTAGAGATG
    TATTTGTGGC TGGGCCTGAA TAAACAGAGG AAAGACTTCC TGATTGGCCT CCCTAGTGGC
    TTTGAAGAAA ACAAGGCAGT AAAGGGAATT GGCATACAAG CTGTCCCTCC CATCAGCCTA
    GTTTATAACA TGAAGCAGGT GTTTCAGCTG AGGGCCCACA TGTATCAAGC TCGCAGTCTG
    TTTGCTGCGG ACACTAGTGG CCTGTCTGAT CCATTTGCAA GGGTTTTCTT CTCCACCCAC
    AGCCAGGTCA CGGAGGTCCT CAGTGAGACT CTCTGTCCAA CATGGGATCA GCTGCTTGTG
    TTTGATAATG TTGAACTTTA CGGTGAGGCC GGTGAACTCC GTGATGACCC TCCAATCATT
    GTCATTGAGT TGTATGATCA AGACACTGTG GGGAAAGCTG AGTTTATTGG CCGAACATTT
    GCAAAACCAC TTACTAAGAT GGTTGATGAA CACTACGGCC CACCGCGGTT TCCTCCCCAG
    CTCGAGTATT ATCAGATCTA CCGAGGAAAC TGTGCTGCGG GAGATCTATT GGCTGCTTTT
    GAGCTTCTAC AGATTCCTTA TGACGATGAG GAGATCAGAA GAGCTCTTAT TGCTGTCCAT
    GACTTTGCTG TGCCTCAGAT CAAGATTGGT CCAGCAGGGC GGGCAGCACT TCCTCCAATC
    GATGGACCGA CCGATTCTGA CCGTGGGCCC ATTCTGCCTG TTCCATTGGG AATTCGACCA
    GTTCTCAGCA GATATCGCAT CGAGGTCCTG TTTTGGGGTC TGAGGGACCT TAAAAGGGTC
    AACCTGGCTC AGGTGGACCG ACCTCGTGTG GACATTGAGT GTGCAGGGAA AGGAGTTCAG
    TCAGCCCTAA TTCAAAACTA CAAGAAGAAT CCAAACTTCA GCACATTAGT AAAGTGGTTT
    GAAGTGGATC TGCCAGAAAA TGAGCTGCTC CATCCACCAC TTAATATTCG GGTAGTGGAT
    TGCAGGGCAT TCGGACGCTA CATTTTGGTG GGGTCTCATG CTGTGACCAC CCTTCGCAAG
    TTCATCTACA GCCCCCCAGA CAAGACTGCT AACAACTGGG CTCACACAGC TAGACTGGCC
    AATGGCTACC TGGCTCTCAG CAATGGGACG TCTCATTCCC GCCCCCAATC CCGTCCTGTT
    TCTTATCCCT CAGGTGACAT TGTGGTCAAC ATGAGCCCTG AACCTAACAT TAAGAAGATG
    GACACGGTTG TCAAGATTGA AGCTACCACC GATGCGGTTG TTAAAGTTGA CTTGAATGAG
    GATGAGAAGG AGAAAGAAAA GAAGAAGAAG AAGAAGAAGA AAGGAGAAGA AGTGGAGGAA
    GAGGAGCCGG ATGAGAGCAT GCTGGACTGG TGGTCCAAAT ACTTTGCCTC AATAGAGACT
    ATGATGGAGA ATCTAAGAGC TCAGGAGGCC GCTCAGGCAG AGGCAGAGGA AAGAGAAGAT
    TTGGAGATTG CAGCAGAGAG TGCAGAGATC AAAGCTGATG ACTTTCCTAT GAAAGGCACC
    AAACCTAAAG AGAAGAGCAA AGACAAGAAG AGCACCAAGG ACAAAAAGAA GAACAACGAT
    GGCACAGAAA AACGACCTCC AAAACCAAAA GTTGATGAAC TCATGGTGTA CAATAAAGAG
    CTGGAGAGTG AGTTCGGTAG CTTTGAGGAC TGGCTTCACA CCTTCAACCT GTACCGAGGA
    AAGGCTGGAG ATGATGATGA CCACAATGTG GTTGATGAGG ACAGAATTGT GGGCAGATTC
    AAGGGCTCCC TCTGTATGTA TAAACTACCA CTGTCTGAGG AGATCACCAG AGAGGCAGGA
    TTTGATCCAA ACATGGGCAT GTTCCAGAGT ATTCCTCACA ATGACCCTAT CAACGTTCTT
    GTCAGGATTT ATATCATTCG AGCCACAGAC CTGCATCCTG CAGATATAAA TGGAAAGGCC
    GACCCTTATA TAGTCATTAA ATTGGGAAAG TCAGACATTC GAGACAAGGA GAACTACATA
    TCCAAGCAGC TGAATCCTGT CTTTGGAAAG TCATTTGATA TAGAGGCCAC GTTCCCAATG
    GAGTCTATGC TGACTGTGGC AGTGTATGAC TGGGATTTAG TCGGAACTGA TGACCTGATT
    GGTGAGACTA AGATTGATTT GGAGAACAGA TACTACAGCA AACACAGAGC CACATGTGGC
    ATTGCTTCCA ACTACTCTGT CCATGGTTAT AATGTATGGA GAGATCCTCA GAAACCAGCT
    CAGATCCTGG CTAAGTTGTG CAAAGAAGGT AAACTGGATG GACCTCACTA TGGACCTGGG
    GGAAGAGTCA AAGTTGCAAA CCGGATCTTT CTTGGGCCGA CAGAAATTGA GGATGAGAGT
    GGTCTGAAAA AACAAACAGA AGAACACTTG GCGCTCACAG TCCTTAGACA TTGGGAGGAA
    ATCCCACGTG TTGGTTGCAA ACTCATCCCT GAGCATGTGG AGACGAGGCC TCTCCTCAAC
    CCAGACAAGC CAGGCATAGA GCAGGGAAGG ATTGAAATGT GGGTGGACAT GTTTCCTATG
    GATGTCCCAG CACCAGGACC AGCCATTGAT ATATCGCCAC GCAAACCAAA GAGCTTTGAG
    CTTCGTGTTA TTATTTGGAA CACTGATGAC GTAATTCTAG AGGACGATGC TTTCATGACA
    GGAGAGAAGA TGTCAGACAT CTATGTCAGG GGCTGGCTTA AAGGACAGCA GGAGGACAAA
    CAGGACACCG ATGTACATTA TCACTCTCTA ACTGGAGAGG GAAACTTCAA CTGGCGCTTT
    GTCTTCCCCT TTGACTATCT CATGGCCGAG GAGAAGATTG TTATCTCCAA GAAGGAATCA
    ATGTTTTCCT GGGATGAGAC TGAGTATAAG ATCCCTGCTC GACTCACCCT TCAAGTATGG
    GATGCCGATC ACTTTTCTGC AGATGATTTT CTGGGTGCGA TTGAGTTGGA CTTGAATAAG
    TTTCCGCGTG GGGCAAAAAC AGCCAAGCAG TGCTCTCTAG ACATGGTTCT CAAAGAGCAT
    GAGCTGCCAA CCATCTCCAT CTTCAAACAA AAGAGAGTCA AGGGCTGGTG GCCATTTGTG
    GCCCAAAATG AGAATGATGA ATTTGAGCTA ACGGGAAAAG TAGAGGCAGA GCTCCATCTG
    TTGACAGCAG AGGAGGCAGA GAAGAGTCCA GTCGGCCTGG GGAGGAATGA TCCGGAGCCA
    CTTGAGAAAC CAAATCGTCC AACTACCAGC CTGTCGTGGC TGTTGAGTCC GATTCGGGTG
    ACCAGCATAA TGTGTTGGCG GCAGTATAAG TTGGTCACAG TGGTGACTCT GCTCATCATA
    ACCGGGCTGA TCTTTTTGGG CCTCCTGTTC TTTTCTCTGC CTTCAGCTTT CATGCAGAAG
    CTCTTCAACA TCTCTGGATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42606
    Clone ID Related Accession (Same CDS sequence) XM_009294734.3 , XM_009294734.2
    Accession Version XM_009294734.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6135bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product otoferlin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009294734.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCTTTAG TGGTGCATCT GAAGACTGTC ACGGAGCTCC GTGGGAAGGG GGACCGAATC 
    GCTAAAGTCA CTTTCAGAGG TCTCTCCTTC TTCTCTCGGG TGTTGGAAAA CTGCGAGGAT
    GAAGCTCGAT TTGAGCAGGC GTTCCGATGG CCAATTGGGA GTCAAGTGGA TGGCGACGAG
    ATGCTGGAGA TCCAAGTGTT TAATTACAGC AAAGTCTTCA CTAATAGGTT AATCGGCACA
    TTCCGCATGG TTCTTCAGAA GGTGGTGGAG GAGGGACACC TGGAGGTGTC TGACACACTC
    ATTGACGACA ATAACACAGC CATACAGACA ACCATTTCCA TAGAGATCAA ATATCAGACC
    ATGGACGGGA GTGTAAAGGT GTGGAGCGAT GGTGAATTCC TTGATATTCC AGATGACCTT
    GATGGAACCT TCCAGTTTGA GACAGATAGT CTTCTCTCAG GACGCAGCCA GAGTTCTGGA
    ACTTCACCTG GACGATCCAT TCATGGCATT CCCACATTCC GCAAGGCAGG AAAAGGAGTG
    TTCTCAGCCA TGAAGCTGGG CAAGACCCGC ACCTCTAAAG ATGACCACAA AAAAGGAGAT
    GATGCTGCCA TTCTGGATGC AGAAGATCTG GATCGAAAAG CCATGCGTCT AGGCGGCATT
    CTTGACCCTG ATACAATCTC ACTGGCCTCG GTTACTGCAG TGACCACTAA TGTCTCCAAC
    AAAAGATCAA AGCCTGACAT TAAAATGGAG CCGAGTTCAG GGCGTCCGGT TGATTATCAG
    ATCAGTGTTA CAGTAATCGA GGCCCGTCAG CTGGTCGGAT TGAACATGGA CCCTGTGGTT
    TGCGTGGAAA TTGGAGAGGA GAAGAAGTAC ACGTCAATGA AGGAGTCAAC AAACTGCCCT
    TACTACAATG AGTATTTTGT CTTCGACTTC CACGTGCCTC CAGACGTCAT GTTTGACAAA
    ATTATAAAGA TTTCAGTAAT ACATTCTAAA AACCTTCTGC GAAGCGGCAC CCTCGTGGGC
    ACCTTCAAAC TAGACGTGGG GACTGTTTAC ACACAACCTG AACATCAGTT TCATCACAAA
    TGGGCGATGC TGTCAGACCC TGATGACATC ACGACTGGCT GCAAAGGTTA TGTTAAATGT
    GACATCGCAG TCGTGGGAAA AGGAGACAAC ATCAAGACTC CACACAAGGC CAGTGAAGCA
    GACGAGGATG ACATAGAGGG GAATCTTCTC TTGCCGGAGG GTGTTCCATC AGAGAGACAG
    TGGGCTCGGT TTTATGTTAA GATTTATAGA GCTGAGGGTC TGCCTAAAAT GAACACCAGT
    ATCATGGCCA ACGTGAAGAA AGCCTTTATT GGGGAAAACA GGGACCTTGT GGACCCTTAC
    GTCCTGGTGC AATTTGCAGG GCAAAAGGGC AAAACATCTG TTCAGAAGAG CAGCTACGAG
    CCCATCTGGA ACGAGCAAGT CATCTTCACG GAGATGTTCC CACCTTTGTG TAGGCGACTG
    AAAGTTCAGA TCCGTGATTC AGACAAGGTG AACGATGTTG CCATAGGAAC CCACTTCATT
    GATCTACGAA AGGTTTCCAA TGATGGAGAC AAAGGTTTCT TGCCCACTAT GGGTCCGGCT
    TGGGTGAATA TGTATGGGTC GACCCGTAAC TACACCCTGA TGGACGAACA CCAGGACCTG
    AATGAGGGGT TGGGGGAAGG GGTGTCCTTC AGAGCACGTC TGCTCATTAG TATCGCGGTG
    GAGATTCTAG ACACCACCTC TGCTGAGATC ATGAGCTCCA CTGAAGTACA CATGGAGCCG
    GTGTCCAACA TCTCAGAGAG TGCTACAGGA AAGATGGAAG AATTTTTCCT TTTCGGGTCA
    TTCTTGGAGG CCACAATGAT AGACAGAAAA ATAGGAGATA AACCCATCAG CTTTGAAGTC
    ACCATCGGTA ACTATGGTAA CCAGATTGAT GGTGTGAGCA AGCCTGCATT AGCGAAGAAG
    AAGAAGGAGG GTGGAGGAGA GAGTGAAGAA GAGGAGTCTG AGCTTATCCA CAACTCCAGC
    GAGGAAGAGG CAGAGGATGA TGGAGATCTG ACCTCTGTAC CGTCTACACC TCCTATGAAG
    CCTGTTATCA CCGACAGAAA TTACTTCCAC TTGCCTTACT TTGAAAAGAA ACCCTGCATT
    TACATCAAGA GCTGGTGGCA GGACCAGCGG AGACGTCTCT ACAACTCCAA CATAATGGAC
    AAGATAGCTG ATAAACTGGA GGAAGGTCTG AATGATGTTC AAGAGATCAT AAAAACAGAG
    AAGGCATATC CAGAACGAAG ACTAAGAGGT GTATTAGAAG AACTTAGCAC AAGCTGCAGC
    CGGTTTGTTA CACTTGCAAA CAAGGATCAG AATCTTTCTG GAAGAACCAA GCTGGACAGA
    GAGAGGCTGA AGTCCTGTAT GAGAGAAATG GAGAGTATGG GTCAGCAGGC GAAGACCATC
    CGCACACAAG TGAAAAGAAA CACAGTTCGA GATAAACTCA AGCTAGTGTT GAATTTCCTT
    CAGAGGCTTC GATTCTTAGC AGACGAGCCC CAGCACAGCA TCCCTGATGT GTTCATATGG
    ATGATTAGCA ACAACAAACG CATCGCATAC GCCCGCATAC CCTCCAAAGA CATCCTCTAC
    TCAATTGTCG ATGAAGAGAT GGGCAAAGAC TGTGGAAAAG TTAAAGCTGT TTTTCTCAGG
    CTGCCTGGAA AGAAAGGTTT TGGGCCTGCT GGCTGGACAG TTCAGGCTAA ATTAGAGATG
    TATTTGTGGC TGGGCCTGAA TAAACAGAGG AAAGACTTCC TGATTGGCCT CCCTAGTGGC
    TTTGAAGAAA ACAAGGCAGT AAAGGGAATT GGCATACAAG CTGTCCCTCC CATCAGCCTA
    GTTTATAACA TGAAGCAGGT GTTTCAGCTG AGGGCCCACA TGTATCAAGC TCGCAGTCTG
    TTTGCTGCGG ACACTAGTGG CCTGTCTGAT CCATTTGCAA GGGTTTTCTT CTCCACCCAC
    AGCCAGGTCA CGGAGGTCCT CAGTGAGACT CTCTGTCCAA CATGGGATCA GCTGCTTGTG
    TTTGATAATG TTGAACTTTA CGGTGAGGCC GGTGAACTCC GTGATGACCC TCCAATCATT
    GTCATTGAGT TGTATGATCA AGACACTGTG GGGAAAGCTG AGTTTATTGG CCGAACATTT
    GCAAAACCAC TTACTAAGAT GGTTGATGAA CACTACGGCC CACCGCGGTT TCCTCCCCAG
    CTCGAGTATT ATCAGATCTA CCGAGGAAAC TGTGCTGCGG GAGATCTATT GGCTGCTTTT
    GAGCTTCTAC AGATTCCTTA TGACGATGAG GAGATCAGAA GAGCTCTTAT TGCTGTCCAT
    GACTTTGCTG TGCCTCAGAT CAAGATTGGT CCAGCAGGGC GGGCAGCACT TCCTCCAATC
    GATGGACCGA CCGATTCTGA CCGTGGGCCC ATTCTGCCTG TTCCATTGGG AATTCGACCA
    GTTCTCAGCA GATATCGCAT CGAGGTCCTG TTTTGGGGTC TGAGGGACCT TAAAAGGGTC
    AACCTGGCTC AGGTGGACCG ACCTCGTGTG GACATTGAGT GTGCAGGGAA AGGAGTTCAG
    TCAGCCCTAA TTCAAAACTA CAAGAAGAAT CCAAACTTCA GCACATTAGT AAAGTGGTTT
    GAAGTGGATC TGCCAGAAAA TGAGCTGCTC CATCCACCAC TTAATATTCG GGTAGTGGAT
    TGCAGGGCAT TCGGACGCTA CATTTTGGTG GGGTCTCATG CTGTGACCAC CCTTCGCAAG
    TTCATCTACA GCCCCCCAGA CAAGACTGCT AACAACTGGG CTCACACAGC TAGACTGGCC
    AATGGCTACC TGGCTCTCAG CAATGGGACG TCTCATTCCC GCCCCCAATC CCGTCCTGTT
    TCTTATCCCT CAGGTGACAT TGTGGTCAAC ATGAGCCCTG AACCTAACAT TAAGAAGATG
    GACACGGTTG TCAAGATTGA AGCTACCACC GATGCGGTTG TTAAAGTTGA CTTGAATGAG
    GATGAGAAGG AGAAAGAAAA GAAGAAGAAG AAGAAGAAGA AAGGAGAAGA AGTGGAGGAA
    GAGGAGCCGG ATGAGAGCAT GCTGGACTGG TGGTCCAAAT ACTTTGCCTC AATAGAGACT
    ATGATGGAGA ATCTAAGAGC TCAGGAGGCC GCTCAGGCAG AGGCAGAGGA AAGAGAAGAT
    TTGGAGATTG CAGCAGAGAG TGCAGAGATC AAAGCTGATG ACTTTCCTAT GAAAGGCACC
    AAACCTAAAG AGAAGAGCAA AGACAAGAAG AGCACCAAGG ACAAAAAGAA GAACAACGAT
    GGCACAGAAA AACGACCTCC AAAACCAAAA GTTGATGAAC TCATGGTGTA CAATAAAGAG
    CTGGAGAGTG AGTTCGGTAG CTTTGAGGAC TGGCTTCACA CCTTCAACCT GTACCGAGGA
    AAGGCTGGAG ATGATGATGA CCACAATGTG GTTGATGAGG ACAGAATTGT GGGCAGATTC
    AAGGGCTCCC TCTGTATGTA TAAACTACCA CTGTCTGAGG AGATCACCAG AGAGGCAGGA
    TTTGATCCAA ACATGGGCAT GTTCCAGAGT ATTCCTCACA ATGACCCTAT CAACGTTCTT
    GTCAGGATTT ATATCATTCG AGCCACAGAC CTGCATCCTG CAGATATAAA TGGAAAGGCC
    GACCCTTATA TAGTCATTAA ATTGGGAAAG TCAGACATTC GAGACAAGGA GAACTACATA
    TCCAAGCAGC TGAATCCTGT CTTTGGAAAG TCATTTGATA TAGAGGCCAC GTTCCCAATG
    GAGTCTATGC TGACTGTGGC AGTGTATGAC TGGGATTTAG TCGGAACTGA TGACCTGATT
    GGTGAGACTA AGATTGATTT GGAGAACAGA TACTACAGCA AACACAGAGC CACATGTGGC
    ATTGCTTCCA ACTACTCTGT CCATGGTTAT AATGTATGGA GAGATCCTCA GAAACCAGCT
    CAGATCCTGG CTAAGTTGTG CAAAGAAGGT AAACTGGATG GACCTCACTA TGGACCTGGG
    GGAAGAGTCA AAGTTGCAAA CCGGATCTTT CTTGGGCCGA CAGAAATTGA GGATGAGAGT
    GGTCTGAAAA AACAAACAGA AGAACACTTG GCGCTCACAG TCCTTAGACA TTGGGAGGAA
    ATCCCACGTG TTGGTTGCAA ACTCATCCCT GAGCATGTGG AGACGAGGCC TCTCCTCAAC
    CCAGACAAGC CAGGCATAGA GCAGGGAAGG ATTGAAATGT GGGTGGACAT GTTTCCTATG
    GATGTCCCAG CACCAGGACC AGCCATTGAT ATATCGCCAC GCAAACCAAA GAGATATGAG
    CTCAGGGTGA TAATATGGAA TACTGACGAG GTAATTCTGG AAGACGATGA TTACTTCACA
    GGGGAAAAGT CCAGTGACAT TTTTGTGAGG GGCTGGCTTA AAGGACAGCA GGAGGACAAA
    CAGGACACCG ATGTACATTA TCACTCTCTA ACTGGAGAGG GAAACTTCAA CTGGCGCTTT
    GTCTTCCCCT TTGACTATCT CATGGCCGAG GAGAAGATTG TTATCTCCAA GAAGGAATCA
    ATGTTTTCCT GGGATGAGAC TGAGTATAAG ATCCCTGCTC GACTCACCCT TCAAGTATGG
    GATGCCGATC ACTTTTCTGC AGATGATTTT CTGGGTGCGA TTGAGTTGGA CTTGAATAAG
    TTTCCGCGTG GGGCAAAAAC AGCCAAGCAG TGCTCTCTAG ACATGGTTCT CAAAGAGCAT
    GAGCTGCCAA CCATCTCCAT CTTCAAACAA AAGAGAGTCA AGGGCTGGTG GCCATTTGTG
    GCCCAAAATG AGAATGATGA ATTTGAGCTA ACGGGAAAAG TAGAGGCAGA GCTCCATCTG
    TTGACAGCAG AGGAGGCAGA GAAGAGTCCA GTCGGCCTGG GGAGGAATGA TCCGGAGCCA
    CTTGAGAAAC CAAACCGGCC CGACACCACC TTCCTGTGGT TTCTGAGCCC ACTGAAGGCC
    ATCCGCTACC TGATATGCAA CCGCTACAAG TGGCTCATCA TCAAGATTGT GTTGGCTCTG
    CTGCTGCTGA TCATGGTGGG CCTGTTTCTC TACAGCATGC CAGGCTATCT GGTCAAGAAG
    CTGCTCGGGG CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009293009.1
    CDS300..6434
    Translation

    Target ORF information:

    RefSeq Version XM_009294734.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio otoferlin a (otofa), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009294734.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCTTTAG TGGTGCATCT GAAGACTGTC ACGGAGCTCC GTGGGAAGGG GGACCGAATC 
    GCTAAAGTCA CTTTCAGAGG TCTCTCCTTC TTCTCTCGGG TGTTGGAAAA CTGCGAGGAT
    GAAGCTCGAT TTGAGCAGGC GTTCCGATGG CCAATTGGGA GTCAAGTGGA TGGCGACGAG
    ATGCTGGAGA TCCAAGTGTT TAATTACAGC AAAGTCTTCA CTAATAGGTT AATCGGCACA
    TTCCGCATGG TTCTTCAGAA GGTGGTGGAG GAGGGACACC TGGAGGTGTC TGACACACTC
    ATTGACGACA ATAACACAGC CATACAGACA ACCATTTCCA TAGAGATCAA ATATCAGACC
    ATGGACGGGA GTGTAAAGGT GTGGAGCGAT GGTGAATTCC TTGATATTCC AGATGACCTT
    GATGGAACCT TCCAGTTTGA GACAGATAGT CTTCTCTCAG GACGCAGCCA GAGTTCTGGA
    ACTTCACCTG GACGATCCAT TCATGGCATT CCCACATTCC GCAAGGCAGG AAAAGGAGTG
    TTCTCAGCCA TGAAGCTGGG CAAGACCCGC ACCTCTAAAG ATGACCACAA AAAAGGAGAT
    GATGCTGCCA TTCTGGATGC AGAAGATCTG GATCGAAAAG CCATGCGTCT AGGCGGCATT
    CTTGACCCTG ATACAATCTC ACTGGCCTCG GTTACTGCAG TGACCACTAA TGTCTCCAAC
    AAAAGATCAA AGCCTGACAT TAAAATGGAG CCGAGTTCAG GGCGTCCGGT TGATTATCAG
    ATCAGTGTTA CAGTAATCGA GGCCCGTCAG CTGGTCGGAT TGAACATGGA CCCTGTGGTT
    TGCGTGGAAA TTGGAGAGGA GAAGAAGTAC ACGTCAATGA AGGAGTCAAC AAACTGCCCT
    TACTACAATG AGTATTTTGT CTTCGACTTC CACGTGCCTC CAGACGTCAT GTTTGACAAA
    ATTATAAAGA TTTCAGTAAT ACATTCTAAA AACCTTCTGC GAAGCGGCAC CCTCGTGGGC
    ACCTTCAAAC TAGACGTGGG GACTGTTTAC ACACAACCTG AACATCAGTT TCATCACAAA
    TGGGCGATGC TGTCAGACCC TGATGACATC ACGACTGGCT GCAAAGGTTA TGTTAAATGT
    GACATCGCAG TCGTGGGAAA AGGAGACAAC ATCAAGACTC CACACAAGGC CAGTGAAGCA
    GACGAGGATG ACATAGAGGG GAATCTTCTC TTGCCGGAGG GTGTTCCATC AGAGAGACAG
    TGGGCTCGGT TTTATGTTAA GATTTATAGA GCTGAGGGTC TGCCTAAAAT GAACACCAGT
    ATCATGGCCA ACGTGAAGAA AGCCTTTATT GGGGAAAACA GGGACCTTGT GGACCCTTAC
    GTCCTGGTGC AATTTGCAGG GCAAAAGGGC AAAACATCTG TTCAGAAGAG CAGCTACGAG
    CCCATCTGGA ACGAGCAAGT CATCTTCACG GAGATGTTCC CACCTTTGTG TAGGCGACTG
    AAAGTTCAGA TCCGTGATTC AGACAAGGTG AACGATGTTG CCATAGGAAC CCACTTCATT
    GATCTACGAA AGGTTTCCAA TGATGGAGAC AAAGGTTTCT TGCCCACTAT GGGTCCGGCT
    TGGGTGAATA TGTATGGGTC GACCCGTAAC TACACCCTGA TGGACGAACA CCAGGACCTG
    AATGAGGGGT TGGGGGAAGG GGTGTCCTTC AGAGCACGTC TGCTCATTAG TATCGCGGTG
    GAGATTCTAG ACACCACCTC TGCTGAGATC ATGAGCTCCA CTGAAGTACA CATGGAGCCG
    GTGTCCAACA TCTCAGAGAG TGCTACAGGA AAGATGGAAG AATTTTTCCT TTTCGGGTCA
    TTCTTGGAGG CCACAATGAT AGACAGAAAA ATAGGAGATA AACCCATCAG CTTTGAAGTC
    ACCATCGGTA ACTATGGTAA CCAGATTGAT GGTGTGAGCA AGCCTGCATT AGCGAAGAAG
    AAGAAGGAGG GTGGAGGAGA GAGTGAAGAA GAGGAGTCTG AGCTTATCCA CAACTCCAGC
    GAGGAAGAGG CAGAGGATGA TGGAGATCTG ACCTCTGTAC CGTCTACACC TCCTATGAAG
    CCTGTTATCA CCGACAGAAA TTACTTCCAC TTGCCTTACT TTGAAAAGAA ACCCTGCATT
    TACATCAAGA GCTGGTGGCA GGACCAGCGG AGACGTCTCT ACAACTCCAA CATAATGGAC
    AAGATAGCTG ATAAACTGGA GGAAGGTCTG AATGATGTTC AAGAGATCAT AAAAACAGAG
    AAGGCATATC CAGAACGAAG ACTAAGAGGT GTATTAGAAG AACTTAGCAC AAGCTGCAGC
    CGGTTTGTTA CACTTGCAAA CAAGGATCAG AATCTTTCTG GAAGAACCAA GCTGGACAGA
    GAGAGGCTGA AGTCCTGTAT GAGAGAAATG GAGAGTATGG GTCAGCAGGC GAAGACCATC
    CGCACACAAG TGAAAAGAAA CACAGTTCGA GATAAACTCA AGCTAGTGTT GAATTTCCTT
    CAGAGGCTTC GATTCTTAGC AGACGAGCCC CAGCACAGCA TCCCTGATGT GTTCATATGG
    ATGATTAGCA ACAACAAACG CATCGCATAC GCCCGCATAC CCTCCAAAGA CATCCTCTAC
    TCAATTGTCG ATGAAGAGAT GGGCAAAGAC TGTGGAAAAG TTAAAGCTGT TTTTCTCAGG
    CTGCCTGGAA AGAAAGGTTT TGGGCCTGCT GGCTGGACAG TTCAGGCTAA ATTAGAGATG
    TATTTGTGGC TGGGCCTGAA TAAACAGAGG AAAGACTTCC TGATTGGCCT CCCTAGTGGC
    TTTGAAGAAA ACAAGGCAGT AAAGGGAATT GGCATACAAG CTGTCCCTCC CATCAGCCTA
    GTTTATAACA TGAAGCAGGT GTTTCAGCTG AGGGCCCACA TGTATCAAGC TCGCAGTCTG
    TTTGCTGCGG ACACTAGTGG CCTGTCTGAT CCATTTGCAA GGGTTTTCTT CTCCACCCAC
    AGCCAGGTCA CGGAGGTCCT CAGTGAGACT CTCTGTCCAA CATGGGATCA GCTGCTTGTG
    TTTGATAATG TTGAACTTTA CGGTGAGGCC GGTGAACTCC GTGATGACCC TCCAATCATT
    GTCATTGAGT TGTATGATCA AGACACTGTG GGGAAAGCTG AGTTTATTGG CCGAACATTT
    GCAAAACCAC TTACTAAGAT GGTTGATGAA CACTACGGCC CACCGCGGTT TCCTCCCCAG
    CTCGAGTATT ATCAGATCTA CCGAGGAAAC TGTGCTGCGG GAGATCTATT GGCTGCTTTT
    GAGCTTCTAC AGATTCCTTA TGACGATGAG GAGATCAGAA GAGCTCTTAT TGCTGTCCAT
    GACTTTGCTG TGCCTCAGAT CAAGATTGGT CCAGCAGGGC GGGCAGCACT TCCTCCAATC
    GATGGACCGA CCGATTCTGA CCGTGGGCCC ATTCTGCCTG TTCCATTGGG AATTCGACCA
    GTTCTCAGCA GATATCGCAT CGAGGTCCTG TTTTGGGGTC TGAGGGACCT TAAAAGGGTC
    AACCTGGCTC AGGTGGACCG ACCTCGTGTG GACATTGAGT GTGCAGGGAA AGGAGTTCAG
    TCAGCCCTAA TTCAAAACTA CAAGAAGAAT CCAAACTTCA GCACATTAGT AAAGTGGTTT
    GAAGTGGATC TGCCAGAAAA TGAGCTGCTC CATCCACCAC TTAATATTCG GGTAGTGGAT
    TGCAGGGCAT TCGGACGCTA CATTTTGGTG GGGTCTCATG CTGTGACCAC CCTTCGCAAG
    TTCATCTACA GCCCCCCAGA CAAGACTGCT AACAACTGGG CTCACACAGC TAGACTGGCC
    AATGGCTACC TGGCTCTCAG CAATGGGACG TCTCATTCCC GCCCCCAATC CCGTCCTGTT
    TCTTATCCCT CAGGTGACAT TGTGGTCAAC ATGAGCCCTG AACCTAACAT TAAGAAGATG
    GACACGGTTG TCAAGATTGA AGCTACCACC GATGCGGTTG TTAAAGTTGA CTTGAATGAG
    GATGAGAAGG AGAAAGAAAA GAAGAAGAAG AAGAAGAAGA AAGGAGAAGA AGTGGAGGAA
    GAGGAGCCGG ATGAGAGCAT GCTGGACTGG TGGTCCAAAT ACTTTGCCTC AATAGAGACT
    ATGATGGAGA ATCTAAGAGC TCAGGAGGCC GCTCAGGCAG AGGCAGAGGA AAGAGAAGAT
    TTGGAGATTG CAGCAGAGAG TGCAGAGATC AAAGCTGATG ACTTTCCTAT GAAAGGCACC
    AAACCTAAAG AGAAGAGCAA AGACAAGAAG AGCACCAAGG ACAAAAAGAA GAACAACGAT
    GGCACAGAAA AACGACCTCC AAAACCAAAA GTTGATGAAC TCATGGTGTA CAATAAAGAG
    CTGGAGAGTG AGTTCGGTAG CTTTGAGGAC TGGCTTCACA CCTTCAACCT GTACCGAGGA
    AAGGCTGGAG ATGATGATGA CCACAATGTG GTTGATGAGG ACAGAATTGT GGGCAGATTC
    AAGGGCTCCC TCTGTATGTA TAAACTACCA CTGTCTGAGG AGATCACCAG AGAGGCAGGA
    TTTGATCCAA ACATGGGCAT GTTCCAGAGT ATTCCTCACA ATGACCCTAT CAACGTTCTT
    GTCAGGATTT ATATCATTCG AGCCACAGAC CTGCATCCTG CAGATATAAA TGGAAAGGCC
    GACCCTTATA TAGTCATTAA ATTGGGAAAG TCAGACATTC GAGACAAGGA GAACTACATA
    TCCAAGCAGC TGAATCCTGT CTTTGGAAAG TCATTTGATA TAGAGGCCAC GTTCCCAATG
    GAGTCTATGC TGACTGTGGC AGTGTATGAC TGGGATTTAG TCGGAACTGA TGACCTGATT
    GGTGAGACTA AGATTGATTT GGAGAACAGA TACTACAGCA AACACAGAGC CACATGTGGC
    ATTGCTTCCA ACTACTCTGT CCATGGTTAT AATGTATGGA GAGATCCTCA GAAACCAGCT
    CAGATCCTGG CTAAGTTGTG CAAAGAAGGT AAACTGGATG GACCTCACTA TGGACCTGGG
    GGAAGAGTCA AAGTTGCAAA CCGGATCTTT CTTGGGCCGA CAGAAATTGA GGATGAGAGT
    GGTCTGAAAA AACAAACAGA AGAACACTTG GCGCTCACAG TCCTTAGACA TTGGGAGGAA
    ATCCCACGTG TTGGTTGCAA ACTCATCCCT GAGCATGTGG AGACGAGGCC TCTCCTCAAC
    CCAGACAAGC CAGGCATAGA GCAGGGAAGG ATTGAAATGT GGGTGGACAT GTTTCCTATG
    GATGTCCCAG CACCAGGACC AGCCATTGAT ATATCGCCAC GCAAACCAAA GAGATATGAG
    CTCAGGGTGA TAATATGGAA TACTGACGAG GTAATTCTGG AAGACGATGA TTACTTCACA
    GGGGAAAAGT CCAGTGACAT TTTTGTGAGG GGCTGGCTTA AAGGACAGCA GGAGGACAAA
    CAGGACACCG ATGTACATTA TCACTCTCTA ACTGGAGAGG GAAACTTCAA CTGGCGCTTT
    GTCTTCCCCT TTGACTATCT CATGGCCGAG GAGAAGATTG TTATCTCCAA GAAGGAATCA
    ATGTTTTCCT GGGATGAGAC TGAGTATAAG ATCCCTGCTC GACTCACCCT TCAAGTATGG
    GATGCCGATC ACTTTTCTGC AGATGATTTT CTGGGTGCGA TTGAGTTGGA CTTGAATAAG
    TTTCCGCGTG GGGCAAAAAC AGCCAAGCAG TGCTCTCTAG ACATGGTTCT CAAAGAGCAT
    GAGCTGCCAA CCATCTCCAT CTTCAAACAA AAGAGAGTCA AGGGCTGGTG GCCATTTGTG
    GCCCAAAATG AGAATGATGA ATTTGAGCTA ACGGGAAAAG TAGAGGCAGA GCTCCATCTG
    TTGACAGCAG AGGAGGCAGA GAAGAGTCCA GTCGGCCTGG GGAGGAATGA TCCGGAGCCA
    CTTGAGAAAC CAAACCGGCC CGACACCACC TTCCTGTGGT TTCTGAGCCC ACTGAAGGCC
    ATCCGCTACC TGATATGCAA CCGCTACAAG TGGCTCATCA TCAAGATTGT GTTGGCTCTG
    CTGCTGCTGA TCATGGTGGG CCTGTTTCTC TACAGCATGC CAGGCTATCT GGTCAAGAAG
    CTGCTCGGGG CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42606
    Clone ID Related Accession (Same CDS sequence) XM_009294734.3 , XM_009294734.2
    Accession Version XM_009294734.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6135bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product otoferlin isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009294734.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCTTTAG TGGTGCATCT GAAGACTGTC ACGGAGCTCC GTGGGAAGGG GGACCGAATC 
    GCTAAAGTCA CTTTCAGAGG TCTCTCCTTC TTCTCTCGGG TGTTGGAAAA CTGCGAGGAT
    GAAGCTCGAT TTGAGCAGGC GTTCCGATGG CCAATTGGGA GTCAAGTGGA TGGCGACGAG
    ATGCTGGAGA TCCAAGTGTT TAATTACAGC AAAGTCTTCA CTAATAGGTT AATCGGCACA
    TTCCGCATGG TTCTTCAGAA GGTGGTGGAG GAGGGACACC TGGAGGTGTC TGACACACTC
    ATTGACGACA ATAACACAGC CATACAGACA ACCATTTCCA TAGAGATCAA ATATCAGACC
    ATGGACGGGA GTGTAAAGGT GTGGAGCGAT GGTGAATTCC TTGATATTCC AGATGACCTT
    GATGGAACCT TCCAGTTTGA GACAGATAGT CTTCTCTCAG GACGCAGCCA GAGTTCTGGA
    ACTTCACCTG GACGATCCAT TCATGGCATT CCCACATTCC GCAAGGCAGG AAAAGGAGTG
    TTCTCAGCCA TGAAGCTGGG CAAGACCCGC ACCTCTAAAG ATGACCACAA AAAAGGAGAT
    GATGCTGCCA TTCTGGATGC AGAAGATCTG GATCGAAAAG CCATGCGTCT AGGCGGCATT
    CTTGACCCTG ATACAATCTC ACTGGCCTCG GTTACTGCAG TGACCACTAA TGTCTCCAAC
    AAAAGATCAA AGCCTGACAT TAAAATGGAG CCGAGTTCAG GGCGTCCGGT TGATTATCAG
    ATCAGTGTTA CAGTAATCGA GGCCCGTCAG CTGGTCGGAT TGAACATGGA CCCTGTGGTT
    TGCGTGGAAA TTGGAGAGGA GAAGAAGTAC ACGTCAATGA AGGAGTCAAC AAACTGCCCT
    TACTACAATG AGTATTTTGT CTTCGACTTC CACGTGCCTC CAGACGTCAT GTTTGACAAA
    ATTATAAAGA TTTCAGTAAT ACATTCTAAA AACCTTCTGC GAAGCGGCAC CCTCGTGGGC
    ACCTTCAAAC TAGACGTGGG GACTGTTTAC ACACAACCTG AACATCAGTT TCATCACAAA
    TGGGCGATGC TGTCAGACCC TGATGACATC ACGACTGGCT GCAAAGGTTA TGTTAAATGT
    GACATCGCAG TCGTGGGAAA AGGAGACAAC ATCAAGACTC CACACAAGGC CAGTGAAGCA
    GACGAGGATG ACATAGAGGG GAATCTTCTC TTGCCGGAGG GTGTTCCATC AGAGAGACAG
    TGGGCTCGGT TTTATGTTAA GATTTATAGA GCTGAGGGTC TGCCTAAAAT GAACACCAGT
    ATCATGGCCA ACGTGAAGAA AGCCTTTATT GGGGAAAACA GGGACCTTGT GGACCCTTAC
    GTCCTGGTGC AATTTGCAGG GCAAAAGGGC AAAACATCTG TTCAGAAGAG CAGCTACGAG
    CCCATCTGGA ACGAGCAAGT CATCTTCACG GAGATGTTCC CACCTTTGTG TAGGCGACTG
    AAAGTTCAGA TCCGTGATTC AGACAAGGTG AACGATGTTG CCATAGGAAC CCACTTCATT
    GATCTACGAA AGGTTTCCAA TGATGGAGAC AAAGGTTTCT TGCCCACTAT GGGTCCGGCT
    TGGGTGAATA TGTATGGGTC GACCCGTAAC TACACCCTGA TGGACGAACA CCAGGACCTG
    AATGAGGGGT TGGGGGAAGG GGTGTCCTTC AGAGCACGTC TGCTCATTAG TATCGCGGTG
    GAGATTCTAG ACACCACCTC TGCTGAGATC ATGAGCTCCA CTGAAGTACA CATGGAGCCG
    GTGTCCAACA TCTCAGAGAG TGCTACAGGA AAGATGGAAG AATTTTTCCT TTTCGGGTCA
    TTCTTGGAGG CCACAATGAT AGACAGAAAA ATAGGAGATA AACCCATCAG CTTTGAAGTC
    ACCATCGGTA ACTATGGTAA CCAGATTGAT GGTGTGAGCA AGCCTGCATT AGCGAAGAAG
    AAGAAGGAGG GTGGAGGAGA GAGTGAAGAA GAGGAGTCTG AGCTTATCCA CAACTCCAGC
    GAGGAAGAGG CAGAGGATGA TGGAGATCTG ACCTCTGTAC CGTCTACACC TCCTATGAAG
    CCTGTTATCA CCGACAGAAA TTACTTCCAC TTGCCTTACT TTGAAAAGAA ACCCTGCATT
    TACATCAAGA GCTGGTGGCA GGACCAGCGG AGACGTCTCT ACAACTCCAA CATAATGGAC
    AAGATAGCTG ATAAACTGGA GGAAGGTCTG AATGATGTTC AAGAGATCAT AAAAACAGAG
    AAGGCATATC CAGAACGAAG ACTAAGAGGT GTATTAGAAG AACTTAGCAC AAGCTGCAGC
    CGGTTTGTTA CACTTGCAAA CAAGGATCAG AATCTTTCTG GAAGAACCAA GCTGGACAGA
    GAGAGGCTGA AGTCCTGTAT GAGAGAAATG GAGAGTATGG GTCAGCAGGC GAAGACCATC
    CGCACACAAG TGAAAAGAAA CACAGTTCGA GATAAACTCA AGCTAGTGTT GAATTTCCTT
    CAGAGGCTTC GATTCTTAGC AGACGAGCCC CAGCACAGCA TCCCTGATGT GTTCATATGG
    ATGATTAGCA ACAACAAACG CATCGCATAC GCCCGCATAC CCTCCAAAGA CATCCTCTAC
    TCAATTGTCG ATGAAGAGAT GGGCAAAGAC TGTGGAAAAG TTAAAGCTGT TTTTCTCAGG
    CTGCCTGGAA AGAAAGGTTT TGGGCCTGCT GGCTGGACAG TTCAGGCTAA ATTAGAGATG
    TATTTGTGGC TGGGCCTGAA TAAACAGAGG AAAGACTTCC TGATTGGCCT CCCTAGTGGC
    TTTGAAGAAA ACAAGGCAGT AAAGGGAATT GGCATACAAG CTGTCCCTCC CATCAGCCTA
    GTTTATAACA TGAAGCAGGT GTTTCAGCTG AGGGCCCACA TGTATCAAGC TCGCAGTCTG
    TTTGCTGCGG ACACTAGTGG CCTGTCTGAT CCATTTGCAA GGGTTTTCTT CTCCACCCAC
    AGCCAGGTCA CGGAGGTCCT CAGTGAGACT CTCTGTCCAA CATGGGATCA GCTGCTTGTG
    TTTGATAATG TTGAACTTTA CGGTGAGGCC GGTGAACTCC GTGATGACCC TCCAATCATT
    GTCATTGAGT TGTATGATCA AGACACTGTG GGGAAAGCTG AGTTTATTGG CCGAACATTT
    GCAAAACCAC TTACTAAGAT GGTTGATGAA CACTACGGCC CACCGCGGTT TCCTCCCCAG
    CTCGAGTATT ATCAGATCTA CCGAGGAAAC TGTGCTGCGG GAGATCTATT GGCTGCTTTT
    GAGCTTCTAC AGATTCCTTA TGACGATGAG GAGATCAGAA GAGCTCTTAT TGCTGTCCAT
    GACTTTGCTG TGCCTCAGAT CAAGATTGGT CCAGCAGGGC GGGCAGCACT TCCTCCAATC
    GATGGACCGA CCGATTCTGA CCGTGGGCCC ATTCTGCCTG TTCCATTGGG AATTCGACCA
    GTTCTCAGCA GATATCGCAT CGAGGTCCTG TTTTGGGGTC TGAGGGACCT TAAAAGGGTC
    AACCTGGCTC AGGTGGACCG ACCTCGTGTG GACATTGAGT GTGCAGGGAA AGGAGTTCAG
    TCAGCCCTAA TTCAAAACTA CAAGAAGAAT CCAAACTTCA GCACATTAGT AAAGTGGTTT
    GAAGTGGATC TGCCAGAAAA TGAGCTGCTC CATCCACCAC TTAATATTCG GGTAGTGGAT
    TGCAGGGCAT TCGGACGCTA CATTTTGGTG GGGTCTCATG CTGTGACCAC CCTTCGCAAG
    TTCATCTACA GCCCCCCAGA CAAGACTGCT AACAACTGGG CTCACACAGC TAGACTGGCC
    AATGGCTACC TGGCTCTCAG CAATGGGACG TCTCATTCCC GCCCCCAATC CCGTCCTGTT
    TCTTATCCCT CAGGTGACAT TGTGGTCAAC ATGAGCCCTG AACCTAACAT TAAGAAGATG
    GACACGGTTG TCAAGATTGA AGCTACCACC GATGCGGTTG TTAAAGTTGA CTTGAATGAG
    GATGAGAAGG AGAAAGAAAA GAAGAAGAAG AAGAAGAAGA AAGGAGAAGA AGTGGAGGAA
    GAGGAGCCGG ATGAGAGCAT GCTGGACTGG TGGTCCAAAT ACTTTGCCTC AATAGAGACT
    ATGATGGAGA ATCTAAGAGC TCAGGAGGCC GCTCAGGCAG AGGCAGAGGA AAGAGAAGAT
    TTGGAGATTG CAGCAGAGAG TGCAGAGATC AAAGCTGATG ACTTTCCTAT GAAAGGCACC
    AAACCTAAAG AGAAGAGCAA AGACAAGAAG AGCACCAAGG ACAAAAAGAA GAACAACGAT
    GGCACAGAAA AACGACCTCC AAAACCAAAA GTTGATGAAC TCATGGTGTA CAATAAAGAG
    CTGGAGAGTG AGTTCGGTAG CTTTGAGGAC TGGCTTCACA CCTTCAACCT GTACCGAGGA
    AAGGCTGGAG ATGATGATGA CCACAATGTG GTTGATGAGG ACAGAATTGT GGGCAGATTC
    AAGGGCTCCC TCTGTATGTA TAAACTACCA CTGTCTGAGG AGATCACCAG AGAGGCAGGA
    TTTGATCCAA ACATGGGCAT GTTCCAGAGT ATTCCTCACA ATGACCCTAT CAACGTTCTT
    GTCAGGATTT ATATCATTCG AGCCACAGAC CTGCATCCTG CAGATATAAA TGGAAAGGCC
    GACCCTTATA TAGTCATTAA ATTGGGAAAG TCAGACATTC GAGACAAGGA GAACTACATA
    TCCAAGCAGC TGAATCCTGT CTTTGGAAAG TCATTTGATA TAGAGGCCAC GTTCCCAATG
    GAGTCTATGC TGACTGTGGC AGTGTATGAC TGGGATTTAG TCGGAACTGA TGACCTGATT
    GGTGAGACTA AGATTGATTT GGAGAACAGA TACTACAGCA AACACAGAGC CACATGTGGC
    ATTGCTTCCA ACTACTCTGT CCATGGTTAT AATGTATGGA GAGATCCTCA GAAACCAGCT
    CAGATCCTGG CTAAGTTGTG CAAAGAAGGT AAACTGGATG GACCTCACTA TGGACCTGGG
    GGAAGAGTCA AAGTTGCAAA CCGGATCTTT CTTGGGCCGA CAGAAATTGA GGATGAGAGT
    GGTCTGAAAA AACAAACAGA AGAACACTTG GCGCTCACAG TCCTTAGACA TTGGGAGGAA
    ATCCCACGTG TTGGTTGCAA ACTCATCCCT GAGCATGTGG AGACGAGGCC TCTCCTCAAC
    CCAGACAAGC CAGGCATAGA GCAGGGAAGG ATTGAAATGT GGGTGGACAT GTTTCCTATG
    GATGTCCCAG CACCAGGACC AGCCATTGAT ATATCGCCAC GCAAACCAAA GAGATATGAG
    CTCAGGGTGA TAATATGGAA TACTGACGAG GTAATTCTGG AAGACGATGA TTACTTCACA
    GGGGAAAAGT CCAGTGACAT TTTTGTGAGG GGCTGGCTTA AAGGACAGCA GGAGGACAAA
    CAGGACACCG ATGTACATTA TCACTCTCTA ACTGGAGAGG GAAACTTCAA CTGGCGCTTT
    GTCTTCCCCT TTGACTATCT CATGGCCGAG GAGAAGATTG TTATCTCCAA GAAGGAATCA
    ATGTTTTCCT GGGATGAGAC TGAGTATAAG ATCCCTGCTC GACTCACCCT TCAAGTATGG
    GATGCCGATC ACTTTTCTGC AGATGATTTT CTGGGTGCGA TTGAGTTGGA CTTGAATAAG
    TTTCCGCGTG GGGCAAAAAC AGCCAAGCAG TGCTCTCTAG ACATGGTTCT CAAAGAGCAT
    GAGCTGCCAA CCATCTCCAT CTTCAAACAA AAGAGAGTCA AGGGCTGGTG GCCATTTGTG
    GCCCAAAATG AGAATGATGA ATTTGAGCTA ACGGGAAAAG TAGAGGCAGA GCTCCATCTG
    TTGACAGCAG AGGAGGCAGA GAAGAGTCCA GTCGGCCTGG GGAGGAATGA TCCGGAGCCA
    CTTGAGAAAC CAAACCGGCC CGACACCACC TTCCTGTGGT TTCTGAGCCC ACTGAAGGCC
    ATCCGCTACC TGATATGCAA CCGCTACAAG TGGCTCATCA TCAAGATTGT GTTGGCTCTG
    CTGCTGCTGA TCATGGTGGG CCTGTTTCTC TACAGCATGC CAGGCTATCT GGTCAAGAAG
    CTGCTCGGGG CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009293009.1
    CDS303..6437
    Translation

    Target ORF information:

    RefSeq Version XM_009294734.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio otoferlin a (otofa), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009294734.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCTTTAG TGGTGCATCT GAAGACTGTC ACGGAGCTCC GTGGGAAGGG GGACCGAATC 
    GCTAAAGTCA CTTTCAGAGG TCTCTCCTTC TTCTCTCGGG TGTTGGAAAA CTGCGAGGAT
    GAAGCTCGAT TTGAGCAGGC GTTCCGATGG CCAATTGGGA GTCAAGTGGA TGGCGACGAG
    ATGCTGGAGA TCCAAGTGTT TAATTACAGC AAAGTCTTCA CTAATAGGTT AATCGGCACA
    TTCCGCATGG TTCTTCAGAA GGTGGTGGAG GAGGGACACC TGGAGGTGTC TGACACACTC
    ATTGACGACA ATAACACAGC CATACAGACA ACCATTTCCA TAGAGATCAA ATATCAGACC
    ATGGACGGGA GTGTAAAGGT GTGGAGCGAT GGTGAATTCC TTGATATTCC AGATGACCTT
    GATGGAACCT TCCAGTTTGA GACAGATAGT CTTCTCTCAG GACGCAGCCA GAGTTCTGGA
    ACTTCACCTG GACGATCCAT TCATGGCATT CCCACATTCC GCAAGGCAGG AAAAGGAGTG
    TTCTCAGCCA TGAAGCTGGG CAAGACCCGC ACCTCTAAAG ATGACCACAA AAAAGGAGAT
    GATGCTGCCA TTCTGGATGC AGAAGATCTG GATCGAAAAG CCATGCGTCT AGGCGGCATT
    CTTGACCCTG ATACAATCTC ACTGGCCTCG GTTACTGCAG TGACCACTAA TGTCTCCAAC
    AAAAGATCAA AGCCTGACAT TAAAATGGAG CCGAGTTCAG GGCGTCCGGT TGATTATCAG
    ATCAGTGTTA CAGTAATCGA GGCCCGTCAG CTGGTCGGAT TGAACATGGA CCCTGTGGTT
    TGCGTGGAAA TTGGAGAGGA GAAGAAGTAC ACGTCAATGA AGGAGTCAAC AAACTGCCCT
    TACTACAATG AGTATTTTGT CTTCGACTTC CACGTGCCTC CAGACGTCAT GTTTGACAAA
    ATTATAAAGA TTTCAGTAAT ACATTCTAAA AACCTTCTGC GAAGCGGCAC CCTCGTGGGC
    ACCTTCAAAC TAGACGTGGG GACTGTTTAC ACACAACCTG AACATCAGTT TCATCACAAA
    TGGGCGATGC TGTCAGACCC TGATGACATC ACGACTGGCT GCAAAGGTTA TGTTAAATGT
    GACATCGCAG TCGTGGGAAA AGGAGACAAC ATCAAGACTC CACACAAGGC CAGTGAAGCA
    GACGAGGATG ACATAGAGGG GAATCTTCTC TTGCCGGAGG GTGTTCCATC AGAGAGACAG
    TGGGCTCGGT TTTATGTTAA GATTTATAGA GCTGAGGGTC TGCCTAAAAT GAACACCAGT
    ATCATGGCCA ACGTGAAGAA AGCCTTTATT GGGGAAAACA GGGACCTTGT GGACCCTTAC
    GTCCTGGTGC AATTTGCAGG GCAAAAGGGC AAAACATCTG TTCAGAAGAG CAGCTACGAG
    CCCATCTGGA ACGAGCAAGT CATCTTCACG GAGATGTTCC CACCTTTGTG TAGGCGACTG
    AAAGTTCAGA TCCGTGATTC AGACAAGGTG AACGATGTTG CCATAGGAAC CCACTTCATT
    GATCTACGAA AGGTTTCCAA TGATGGAGAC AAAGGTTTCT TGCCCACTAT GGGTCCGGCT
    TGGGTGAATA TGTATGGGTC GACCCGTAAC TACACCCTGA TGGACGAACA CCAGGACCTG
    AATGAGGGGT TGGGGGAAGG GGTGTCCTTC AGAGCACGTC TGCTCATTAG TATCGCGGTG
    GAGATTCTAG ACACCACCTC TGCTGAGATC ATGAGCTCCA CTGAAGTACA CATGGAGCCG
    GTGTCCAACA TCTCAGAGAG TGCTACAGGA AAGATGGAAG AATTTTTCCT TTTCGGGTCA
    TTCTTGGAGG CCACAATGAT AGACAGAAAA ATAGGAGATA AACCCATCAG CTTTGAAGTC
    ACCATCGGTA ACTATGGTAA CCAGATTGAT GGTGTGAGCA AGCCTGCATT AGCGAAGAAG
    AAGAAGGAGG GTGGAGGAGA GAGTGAAGAA GAGGAGTCTG AGCTTATCCA CAACTCCAGC
    GAGGAAGAGG CAGAGGATGA TGGAGATCTG ACCTCTGTAC CGTCTACACC TCCTATGAAG
    CCTGTTATCA CCGACAGAAA TTACTTCCAC TTGCCTTACT TTGAAAAGAA ACCCTGCATT
    TACATCAAGA GCTGGTGGCA GGACCAGCGG AGACGTCTCT ACAACTCCAA CATAATGGAC
    AAGATAGCTG ATAAACTGGA GGAAGGTCTG AATGATGTTC AAGAGATCAT AAAAACAGAG
    AAGGCATATC CAGAACGAAG ACTAAGAGGT GTATTAGAAG AACTTAGCAC AAGCTGCAGC
    CGGTTTGTTA CACTTGCAAA CAAGGATCAG AATCTTTCTG GAAGAACCAA GCTGGACAGA
    GAGAGGCTGA AGTCCTGTAT GAGAGAAATG GAGAGTATGG GTCAGCAGGC GAAGACCATC
    CGCACACAAG TGAAAAGAAA CACAGTTCGA GATAAACTCA AGCTAGTGTT GAATTTCCTT
    CAGAGGCTTC GATTCTTAGC AGACGAGCCC CAGCACAGCA TCCCTGATGT GTTCATATGG
    ATGATTAGCA ACAACAAACG CATCGCATAC GCCCGCATAC CCTCCAAAGA CATCCTCTAC
    TCAATTGTCG ATGAAGAGAT GGGCAAAGAC TGTGGAAAAG TTAAAGCTGT TTTTCTCAGG
    CTGCCTGGAA AGAAAGGTTT TGGGCCTGCT GGCTGGACAG TTCAGGCTAA ATTAGAGATG
    TATTTGTGGC TGGGCCTGAA TAAACAGAGG AAAGACTTCC TGATTGGCCT CCCTAGTGGC
    TTTGAAGAAA ACAAGGCAGT AAAGGGAATT GGCATACAAG CTGTCCCTCC CATCAGCCTA
    GTTTATAACA TGAAGCAGGT GTTTCAGCTG AGGGCCCACA TGTATCAAGC TCGCAGTCTG
    TTTGCTGCGG ACACTAGTGG CCTGTCTGAT CCATTTGCAA GGGTTTTCTT CTCCACCCAC
    AGCCAGGTCA CGGAGGTCCT CAGTGAGACT CTCTGTCCAA CATGGGATCA GCTGCTTGTG
    TTTGATAATG TTGAACTTTA CGGTGAGGCC GGTGAACTCC GTGATGACCC TCCAATCATT
    GTCATTGAGT TGTATGATCA AGACACTGTG GGGAAAGCTG AGTTTATTGG CCGAACATTT
    GCAAAACCAC TTACTAAGAT GGTTGATGAA CACTACGGCC CACCGCGGTT TCCTCCCCAG
    CTCGAGTATT ATCAGATCTA CCGAGGAAAC TGTGCTGCGG GAGATCTATT GGCTGCTTTT
    GAGCTTCTAC AGATTCCTTA TGACGATGAG GAGATCAGAA GAGCTCTTAT TGCTGTCCAT
    GACTTTGCTG TGCCTCAGAT CAAGATTGGT CCAGCAGGGC GGGCAGCACT TCCTCCAATC
    GATGGACCGA CCGATTCTGA CCGTGGGCCC ATTCTGCCTG TTCCATTGGG AATTCGACCA
    GTTCTCAGCA GATATCGCAT CGAGGTCCTG TTTTGGGGTC TGAGGGACCT TAAAAGGGTC
    AACCTGGCTC AGGTGGACCG ACCTCGTGTG GACATTGAGT GTGCAGGGAA AGGAGTTCAG
    TCAGCCCTAA TTCAAAACTA CAAGAAGAAT CCAAACTTCA GCACATTAGT AAAGTGGTTT
    GAAGTGGATC TGCCAGAAAA TGAGCTGCTC CATCCACCAC TTAATATTCG GGTAGTGGAT
    TGCAGGGCAT TCGGACGCTA CATTTTGGTG GGGTCTCATG CTGTGACCAC CCTTCGCAAG
    TTCATCTACA GCCCCCCAGA CAAGACTGCT AACAACTGGG CTCACACAGC TAGACTGGCC
    AATGGCTACC TGGCTCTCAG CAATGGGACG TCTCATTCCC GCCCCCAATC CCGTCCTGTT
    TCTTATCCCT CAGGTGACAT TGTGGTCAAC ATGAGCCCTG AACCTAACAT TAAGAAGATG
    GACACGGTTG TCAAGATTGA AGCTACCACC GATGCGGTTG TTAAAGTTGA CTTGAATGAG
    GATGAGAAGG AGAAAGAAAA GAAGAAGAAG AAGAAGAAGA AAGGAGAAGA AGTGGAGGAA
    GAGGAGCCGG ATGAGAGCAT GCTGGACTGG TGGTCCAAAT ACTTTGCCTC AATAGAGACT
    ATGATGGAGA ATCTAAGAGC TCAGGAGGCC GCTCAGGCAG AGGCAGAGGA AAGAGAAGAT
    TTGGAGATTG CAGCAGAGAG TGCAGAGATC AAAGCTGATG ACTTTCCTAT GAAAGGCACC
    AAACCTAAAG AGAAGAGCAA AGACAAGAAG AGCACCAAGG ACAAAAAGAA GAACAACGAT
    GGCACAGAAA AACGACCTCC AAAACCAAAA GTTGATGAAC TCATGGTGTA CAATAAAGAG
    CTGGAGAGTG AGTTCGGTAG CTTTGAGGAC TGGCTTCACA CCTTCAACCT GTACCGAGGA
    AAGGCTGGAG ATGATGATGA CCACAATGTG GTTGATGAGG ACAGAATTGT GGGCAGATTC
    AAGGGCTCCC TCTGTATGTA TAAACTACCA CTGTCTGAGG AGATCACCAG AGAGGCAGGA
    TTTGATCCAA ACATGGGCAT GTTCCAGAGT ATTCCTCACA ATGACCCTAT CAACGTTCTT
    GTCAGGATTT ATATCATTCG AGCCACAGAC CTGCATCCTG CAGATATAAA TGGAAAGGCC
    GACCCTTATA TAGTCATTAA ATTGGGAAAG TCAGACATTC GAGACAAGGA GAACTACATA
    TCCAAGCAGC TGAATCCTGT CTTTGGAAAG TCATTTGATA TAGAGGCCAC GTTCCCAATG
    GAGTCTATGC TGACTGTGGC AGTGTATGAC TGGGATTTAG TCGGAACTGA TGACCTGATT
    GGTGAGACTA AGATTGATTT GGAGAACAGA TACTACAGCA AACACAGAGC CACATGTGGC
    ATTGCTTCCA ACTACTCTGT CCATGGTTAT AATGTATGGA GAGATCCTCA GAAACCAGCT
    CAGATCCTGG CTAAGTTGTG CAAAGAAGGT AAACTGGATG GACCTCACTA TGGACCTGGG
    GGAAGAGTCA AAGTTGCAAA CCGGATCTTT CTTGGGCCGA CAGAAATTGA GGATGAGAGT
    GGTCTGAAAA AACAAACAGA AGAACACTTG GCGCTCACAG TCCTTAGACA TTGGGAGGAA
    ATCCCACGTG TTGGTTGCAA ACTCATCCCT GAGCATGTGG AGACGAGGCC TCTCCTCAAC
    CCAGACAAGC CAGGCATAGA GCAGGGAAGG ATTGAAATGT GGGTGGACAT GTTTCCTATG
    GATGTCCCAG CACCAGGACC AGCCATTGAT ATATCGCCAC GCAAACCAAA GAGATATGAG
    CTCAGGGTGA TAATATGGAA TACTGACGAG GTAATTCTGG AAGACGATGA TTACTTCACA
    GGGGAAAAGT CCAGTGACAT TTTTGTGAGG GGCTGGCTTA AAGGACAGCA GGAGGACAAA
    CAGGACACCG ATGTACATTA TCACTCTCTA ACTGGAGAGG GAAACTTCAA CTGGCGCTTT
    GTCTTCCCCT TTGACTATCT CATGGCCGAG GAGAAGATTG TTATCTCCAA GAAGGAATCA
    ATGTTTTCCT GGGATGAGAC TGAGTATAAG ATCCCTGCTC GACTCACCCT TCAAGTATGG
    GATGCCGATC ACTTTTCTGC AGATGATTTT CTGGGTGCGA TTGAGTTGGA CTTGAATAAG
    TTTCCGCGTG GGGCAAAAAC AGCCAAGCAG TGCTCTCTAG ACATGGTTCT CAAAGAGCAT
    GAGCTGCCAA CCATCTCCAT CTTCAAACAA AAGAGAGTCA AGGGCTGGTG GCCATTTGTG
    GCCCAAAATG AGAATGATGA ATTTGAGCTA ACGGGAAAAG TAGAGGCAGA GCTCCATCTG
    TTGACAGCAG AGGAGGCAGA GAAGAGTCCA GTCGGCCTGG GGAGGAATGA TCCGGAGCCA
    CTTGAGAAAC CAAACCGGCC CGACACCACC TTCCTGTGGT TTCTGAGCCC ACTGAAGGCC
    ATCCGCTACC TGATATGCAA CCGCTACAAG TGGCTCATCA TCAAGATTGT GTTGGCTCTG
    CTGCTGCTGA TCATGGTGGG CCTGTTTCTC TACAGCATGC CAGGCTATCT GGTCAAGAAG
    CTGCTCGGGG CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42607
    Clone ID Related Accession (Same CDS sequence) XM_009294736.3 , XM_009294735.2 , XM_009294736.2
    Accession Version XM_009294736.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6135bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product otoferlin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009294736.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCTTTAG TGGTGCATCT GAAGACTGTC ACGGAGCTCC GTGGGAAGGG GGACCGAATC 
    GCTAAAGTCA CTTTCAGAGG TCTCTCCTTC TTCTCTCGGG TGTTGGAAAA CTGCGAGGAT
    GAAGCTCGAT TTGAGCAGGC GTTCCGATGG CCAATTGGGA GTCAAGTGGA TGGCGACGAG
    ATGCTGGAGA TCCAAGTGTT TAATTACAGC AAAGTCTTCA CTAATAGGTT AATCGGCACA
    TTCCGCATGG TTCTTCAGAA GGTGGTGGAG GAGGGACACC TGGAGGTGTC TGACACACTC
    ATTGACGACA ATAACACAGC CATACAGACA ACCATTTCCA TAGAGATCAA ATATCAGACC
    ATGGACGGGA GTGTAAAGGT GTGGAGCGAT GGTGAATTCC TTGATATTCC AGATGACCTT
    GATGGAACCT TCCAGTTTGA GACAGATAGT CTTCTCTCAG GACGCAGCCA GAGTTCTGGA
    ACTTCACCTG GACGATCCAT TCATGGCATT CCCACATTCC GCAAGGCAGG AAAAGGAGTG
    TTCTCAGCCA TGAAGCTGGG CAAGACCCGC ACCTCTAAAG ATGACCACAA AAAAGGAGAT
    GATGCTGCCA TTCTGGATGC AGAAGATCTG GATCGAAAAG CCATGCGTCT AGGCGGCATT
    CTTGACCCTG ATACAATCTC ACTGGCCTCG GTTACTGCAG TGACCACTAA TGTCTCCAAC
    AAAAGATCAA AGCCTGACAT TAAAATGGAG CCGAGTTCAG GGCGTCCGGT TGATTATCAG
    ATCAGTGTTA CAGTAATCGA GGCCCGTCAG CTGGTCGGAT TGAACATGGA CCCTGTGGTT
    TGCGTGGAAA TTGGAGAGGA GAAGAAGTAC ACGTCAATGA AGGAGTCAAC AAACTGCCCT
    TACTACAATG AGTATTTTGT CTTCGACTTC CACGTGCCTC CAGACGTCAT GTTTGACAAA
    ATTATAAAGA TTTCAGTAAT ACATTCTAAA AACCTTCTGC GAAGCGGCAC CCTCGTGGGC
    ACCTTCAAAC TAGACGTGGG GACTGTTTAC ACACAACCTG AACATCAGTT TCATCACAAA
    TGGGCGATGC TGTCAGACCC TGATGACATC ACGACTGGCT GCAAAGGTTA TGTTAAATGT
    GACATCGCAG TCGTGGGAAA AGGAGACAAC ATCAAGACTC CACACAAGGC CAGTGAAGCA
    GACGAGGATG ACATAGAGGG GAATCTTCTC TTGCCGGAGG GTGTTCCATC AGAGAGACAG
    TGGGCTCGGT TTTATGTTAA GATTTATAGA GCTGAGGGTC TGCCTAAAAT GAACACCAGT
    ATCATGGCCA ACGTGAAGAA AGCCTTTATT GGGGAAAACA GGGACCTTGT GGACCCTTAC
    GTCCTGGTGC AATTTGCAGG GCAAAAGGGC AAAACATCTG TTCAGAAGAG CAGCTACGAG
    CCCATCTGGA ACGAGCAAGT CATCTTCACG GAGATGTTCC CACCTTTGTG TAGGCGACTG
    AAAGTTCAGA TCCGTGATTC AGACAAGGTG AACGATGTTG CCATAGGAAC CCACTTCATT
    GATCTACGAA AGGTTTCCAA TGATGGAGAC AAAGGTTTCT TGCCCACTAT GGGTCCGGCT
    TGGGTGAATA TGTATGGGTC GACCCGTAAC TACACCCTGA TGGACGAACA CCAGGACCTG
    AATGAGGGGT TGGGGGAAGG GGTGTCCTTC AGAGCACGTC TGCTCATTAG TATCGCGGTG
    GAGATTCTAG ACACCACCTC TGCTGAGATC ATGAGCTCCA CTGAAGTACA CATGGAGCCG
    GTGTCCAACA TCTCAGAGAG TGCTACAGGA AAGATGGAAG AATTTTTCCT TTTCGGGTCA
    TTCTTGGAGG CCACAATGAT AGACAGAAAA ATAGGAGATA AACCCATCAG CTTTGAAGTC
    ACCATCGGTA ACTATGGTAA CCAGATTGAT GGTGTGAGCA AGCCTGCATT AGCGAAGAAG
    AAGAAGGAGG GTGGAGGAGA GAGTGAAGAA GAGGAGTCTG AGCTTATCCA CAACTCCAGC
    GAGGAAGAGG CAGAGGATGA TGGAGATCTG ACCTCTGTAC CGTCTACACC TCCTATGAAG
    CCTGTTATCA CCGACAGAAA TTACTTCCAC TTGCCTTACT TTGAAAAGAA ACCCTGCATT
    TACATCAAGA GCTGGTGGCA GGACCAGCGG AGACGTCTCT ACAACTCCAA CATAATGGAC
    AAGATAGCTG ATAAACTGGA GGAAGGTCTG AATGATGTTC AAGAGATCAT AAAAACAGAG
    AAGGCATATC CAGAACGAAG ACTAAGAGGT GTATTAGAAG AACTTAGCAC AAGCTGCAGC
    CGGTTTGTTA CACTTGCAAA CAAGGATCAG AATCTTTCTG GAAGAACCAA GCTGGACAGA
    GAGAGGCTGA AGTCCTGTAT GAGAGAAATG GAGAGTATGG GTCAGCAGGC GAAGACCATC
    CGCACACAAG TGAAAAGAAA CACAGTTCGA GATAAACTCA AGCTAGTGTT GAATTTCCTT
    CAGAGGCTTC GATTCTTAGC AGACGAGCCC CAGCACAGCA TCCCTGATGT GTTCATATGG
    ATGATTAGCA ACAACAAACG CATCGCATAC GCCCGCATAC CCTCCAAAGA CATCCTCTAC
    TCAATTGTCG ATGAAGAGAT GGGCAAAGAC TGTGGAAAAG TTAAAGCTGT TTTTCTCAGG
    CTGCCTGGAA AGAAAGGTTT TGGGCCTGCT GGCTGGACAG TTCAGGCTAA ATTAGAGATG
    TATTTGTGGC TGGGCCTGAA TAAACAGAGG AAAGACTTCC TGATTGGCCT CCCTAGTGGC
    TTTGAAGAAA ACAAGGCAGT AAAGGGAATT GGCATACAAG CTGTCCCTCC CATCAGCCTA
    GTTTATAACA TGAAGCAGGT GTTTCAGCTG AGGGCCCACA TGTATCAAGC TCGCAGTCTG
    TTTGCTGCGG ACACTAGTGG CCTGTCTGAT CCATTTGCAA GGGTTTTCTT CTCCACCCAC
    AGCCAGGTCA CGGAGGTCCT CAGTGAGACT CTCTGTCCAA CATGGGATCA GCTGCTTGTG
    TTTGATAATG TTGAACTTTA CGGTGAGGCC GGTGAACTCC GTGATGACCC TCCAATCATT
    GTCATTGAGT TGTATGATCA AGACACTGTG GGGAAAGCTG AGTTTATTGG CCGAACATTT
    GCAAAACCAC TTACTAAGAT GGTTGATGAA CACTACGGCC CACCGCGGTT TCCTCCCCAG
    CTCGAGTATT ATCAGATCTA CCGAGGAAAC TGTGCTGCGG GAGATCTATT GGCTGCTTTT
    GAGCTTCTAC AGATTCCTTA TGACGATGAG GAGATCAGAA GAGCTCTTAT TGCTGTCCAT
    GACTTTGCTG TGCCTCAGAT CAAGATTGGT CCAGCAGGGC GGGCAGCACT TCCTCCAATC
    GATGGACCGA CCGATTCTGA CCGTGGGCCC ATTCTGCCTG TTCCATTGGG AATTCGACCA
    GTTCTCAGCA GATATCGCAT CGAGGTCCTG TTTTGGGGTC TGAGGGACCT TAAAAGGGTC
    AACCTGGCTC AGGTGGACCG ACCTCGTGTG GACATTGAGT GTGCAGGGAA AGGAGTTCAG
    TCAGCCCTAA TTCAAAACTA CAAGAAGAAT CCAAACTTCA GCACATTAGT AAAGTGGTTT
    GAAGTGGATC TGCCAGAAAA TGAGCTGCTC CATCCACCAC TTAATATTCG GGTAGTGGAT
    TGCAGGGCAT TCGGACGCTA CATTTTGGTG GGGTCTCATG CTGTGACCAC CCTTCGCAAG
    TTCATCTACA GCCCCCCAGA CAAGACTGCT AACAACTGGG CTCACACAGC TAGACTGGCC
    AATGGCTACC TGGCTCTCAG CAATGGGACG TCTCATTCCC GCCCCCAATC CCGTCCTGTT
    TCTTATCCCT CAGGTGACAT TGTGGTCAAC ATGAGCCCTG AACCTAACAT TAAGAAGATG
    GACACGGTTG TCAAGATTGA AGCTACCACC GATGCGGTTG TTAAAGTTGA CTTGAATGAG
    GATGAGAAGG AGAAAGAAAA GAAGAAGAAG AAGAAGAAGA AAGGAGAAGA AGTGGAGGAA
    GAGGAGCCGG ATGAGAGCAT GCTGGACTGG TGGTCCAAAT ACTTTGCCTC AATAGAGACT
    ATGATGGAGA ATCTAAGAGC TCAGGAGGCC GCTCAGGCAG AGGCAGAGGA AAGAGAAGAT
    TTGGAGATTG CAGCAGAGAG TGCAGAGATC AAAGCTGATG ACTTTCCTAT GAAAGGCACC
    AAACCTAAAG AGAAGAGCAA AGACAAGAAG AGCACCAAGG ACAAAAAGAA GAACAACGAT
    GGCACAGAAA AACGACCTCC AAAACCAAAA GTTGATGAAC TCATGGTGTA CAATAAAGAG
    CTGGAGAGTG AGTTCGGTAG CTTTGAGGAC TGGCTTCACA CCTTCAACCT GTACCGAGGA
    AAGGCTGGAG ATGATGATGA CCACAATGTG GTTGATGAGG ACAGAATTGT GGGCAGATTC
    AAGGGCTCCC TCTGTATGTA TAAACTACCA CTGTCTGAGG AGATCACCAG AGAGGCAGGA
    TTTGATCCAA ACATGGGCAT GTTCCAGAGT ATTCCTCACA ATGACCCTAT CAACGTTCTT
    GTCAGGATTT ATATCATTCG AGCCACAGAC CTGCATCCTG CAGATATAAA TGGAAAGGCC
    GACCCTTATA TAGTCATTAA ATTGGGAAAG TCAGACATTC GAGACAAGGA GAACTACATA
    TCCAAGCAGC TGAATCCTGT CTTTGGAAAG TCATTTGATA TAGAGGCCAC GTTCCCAATG
    GAGTCTATGC TGACTGTGGC AGTGTATGAC TGGGATTTAG TCGGAACTGA TGACCTGATT
    GGTGAGACTA AGATTGATTT GGAGAACAGA TACTACAGCA AACACAGAGC CACATGTGGC
    ATTGCTTCCA ACTACTCTGT CCATGGTTAT AATGTATGGA GAGATCCTCA GAAACCAGCT
    CAGATCCTGG CTAAGTTGTG CAAAGAAGGT AAACTGGATG GACCTCACTA TGGACCTGGG
    GGAAGAGTCA AAGTTGCAAA CCGGATCTTT CTTGGGCCGA CAGAAATTGA GGATGAGAGT
    GGTCTGAAAA AACAAACAGA AGAACACTTG GCGCTCACAG TCCTTAGACA TTGGGAGGAA
    ATCCCACGTG TTGGTTGCAA ACTCATCCCT GAGCATGTGG AGACGAGGCC TCTCCTCAAC
    CCAGACAAGC CAGGCATAGA GCAGGGAAGG ATTGAAATGT GGGTGGACAT GTTTCCTATG
    GATGTCCCAG CACCAGGACC AGCCATTGAT ATATCGCCAC GCAAACCAAA GAGATATGAG
    CTCAGGGTGA TAATATGGAA TACTGACGAG GTAATTCTGG AAGACGATGA TTACTTCACA
    GGGGAAAAGT CCAGTGACAT TTTTGTGAGG GGCTGGCTTA AAGGACAGCA GGAGGACAAA
    CAGGACACCG ATGTACATTA TCACTCTCTA ACTGGAGAGG GAAACTTCAA CTGGCGCTTT
    GTCTTCCCCT TTGACTATCT CATGGCCGAG GAGAAGATTG TTATCTCCAA GAAGGAATCA
    ATGTTTTCCT GGGATGAGAC TGAGTATAAG ATCCCTGCTC GACTCACCCT TCAAGTATGG
    GATGCCGATC ACTTTTCTGC AGATGATTTT CTGGGTGCGA TTGAGTTGGA CTTGAATAAG
    TTTCCGCGTG GGGCAAAAAC AGCCAAGCAG TGCTCTCTAG ACATGGTTCT CAAAGAGCAT
    GAGCTGCCAA CCATCTCCAT CTTCAAACAA AAGAGAGTCA AGGGCTGGTG GCCATTTGTG
    GCCCAAAATG AGAATGATGA ATTTGAGCTA ACGGGAAAAG TAGAGGCAGA GCTCCATCTG
    TTGACAGCAG AGGAGGCAGA GAAGAGTCCA GTCGGCCTGG GGAGGAATGA TCCGGAGCCA
    CTTGAGAAAC CAAATCGTCC AGACACCAGT CTAATGTGGT TCATGAACCC TCTGAGGTCC
    ATACGATACT TCATTTGGCA CAACTACCGC TGGCTGATCC TGAAGGCTCT GGCTCTGCTG
    CTGCTCCTTC TCCTCGTTGG GCTCTTCCTC TACTCTATTC CTGGATACCT GGTCAAGAAA
    CTCCTAGGGG CTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009293011.1
    CDS300..6434
    Translation

    Target ORF information:

    RefSeq Version XM_009294736.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio otoferlin a (otofa), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009294736.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCTTTAG TGGTGCATCT GAAGACTGTC ACGGAGCTCC GTGGGAAGGG GGACCGAATC 
    GCTAAAGTCA CTTTCAGAGG TCTCTCCTTC TTCTCTCGGG TGTTGGAAAA CTGCGAGGAT
    GAAGCTCGAT TTGAGCAGGC GTTCCGATGG CCAATTGGGA GTCAAGTGGA TGGCGACGAG
    ATGCTGGAGA TCCAAGTGTT TAATTACAGC AAAGTCTTCA CTAATAGGTT AATCGGCACA
    TTCCGCATGG TTCTTCAGAA GGTGGTGGAG GAGGGACACC TGGAGGTGTC TGACACACTC
    ATTGACGACA ATAACACAGC CATACAGACA ACCATTTCCA TAGAGATCAA ATATCAGACC
    ATGGACGGGA GTGTAAAGGT GTGGAGCGAT GGTGAATTCC TTGATATTCC AGATGACCTT
    GATGGAACCT TCCAGTTTGA GACAGATAGT CTTCTCTCAG GACGCAGCCA GAGTTCTGGA
    ACTTCACCTG GACGATCCAT TCATGGCATT CCCACATTCC GCAAGGCAGG AAAAGGAGTG
    TTCTCAGCCA TGAAGCTGGG CAAGACCCGC ACCTCTAAAG ATGACCACAA AAAAGGAGAT
    GATGCTGCCA TTCTGGATGC AGAAGATCTG GATCGAAAAG CCATGCGTCT AGGCGGCATT
    CTTGACCCTG ATACAATCTC ACTGGCCTCG GTTACTGCAG TGACCACTAA TGTCTCCAAC
    AAAAGATCAA AGCCTGACAT TAAAATGGAG CCGAGTTCAG GGCGTCCGGT TGATTATCAG
    ATCAGTGTTA CAGTAATCGA GGCCCGTCAG CTGGTCGGAT TGAACATGGA CCCTGTGGTT
    TGCGTGGAAA TTGGAGAGGA GAAGAAGTAC ACGTCAATGA AGGAGTCAAC AAACTGCCCT
    TACTACAATG AGTATTTTGT CTTCGACTTC CACGTGCCTC CAGACGTCAT GTTTGACAAA
    ATTATAAAGA TTTCAGTAAT ACATTCTAAA AACCTTCTGC GAAGCGGCAC CCTCGTGGGC
    ACCTTCAAAC TAGACGTGGG GACTGTTTAC ACACAACCTG AACATCAGTT TCATCACAAA
    TGGGCGATGC TGTCAGACCC TGATGACATC ACGACTGGCT GCAAAGGTTA TGTTAAATGT
    GACATCGCAG TCGTGGGAAA AGGAGACAAC ATCAAGACTC CACACAAGGC CAGTGAAGCA
    GACGAGGATG ACATAGAGGG GAATCTTCTC TTGCCGGAGG GTGTTCCATC AGAGAGACAG
    TGGGCTCGGT TTTATGTTAA GATTTATAGA GCTGAGGGTC TGCCTAAAAT GAACACCAGT
    ATCATGGCCA ACGTGAAGAA AGCCTTTATT GGGGAAAACA GGGACCTTGT GGACCCTTAC
    GTCCTGGTGC AATTTGCAGG GCAAAAGGGC AAAACATCTG TTCAGAAGAG CAGCTACGAG
    CCCATCTGGA ACGAGCAAGT CATCTTCACG GAGATGTTCC CACCTTTGTG TAGGCGACTG
    AAAGTTCAGA TCCGTGATTC AGACAAGGTG AACGATGTTG CCATAGGAAC CCACTTCATT
    GATCTACGAA AGGTTTCCAA TGATGGAGAC AAAGGTTTCT TGCCCACTAT GGGTCCGGCT
    TGGGTGAATA TGTATGGGTC GACCCGTAAC TACACCCTGA TGGACGAACA CCAGGACCTG
    AATGAGGGGT TGGGGGAAGG GGTGTCCTTC AGAGCACGTC TGCTCATTAG TATCGCGGTG
    GAGATTCTAG ACACCACCTC TGCTGAGATC ATGAGCTCCA CTGAAGTACA CATGGAGCCG
    GTGTCCAACA TCTCAGAGAG TGCTACAGGA AAGATGGAAG AATTTTTCCT TTTCGGGTCA
    TTCTTGGAGG CCACAATGAT AGACAGAAAA ATAGGAGATA AACCCATCAG CTTTGAAGTC
    ACCATCGGTA ACTATGGTAA CCAGATTGAT GGTGTGAGCA AGCCTGCATT AGCGAAGAAG
    AAGAAGGAGG GTGGAGGAGA GAGTGAAGAA GAGGAGTCTG AGCTTATCCA CAACTCCAGC
    GAGGAAGAGG CAGAGGATGA TGGAGATCTG ACCTCTGTAC CGTCTACACC TCCTATGAAG
    CCTGTTATCA CCGACAGAAA TTACTTCCAC TTGCCTTACT TTGAAAAGAA ACCCTGCATT
    TACATCAAGA GCTGGTGGCA GGACCAGCGG AGACGTCTCT ACAACTCCAA CATAATGGAC
    AAGATAGCTG ATAAACTGGA GGAAGGTCTG AATGATGTTC AAGAGATCAT AAAAACAGAG
    AAGGCATATC CAGAACGAAG ACTAAGAGGT GTATTAGAAG AACTTAGCAC AAGCTGCAGC
    CGGTTTGTTA CACTTGCAAA CAAGGATCAG AATCTTTCTG GAAGAACCAA GCTGGACAGA
    GAGAGGCTGA AGTCCTGTAT GAGAGAAATG GAGAGTATGG GTCAGCAGGC GAAGACCATC
    CGCACACAAG TGAAAAGAAA CACAGTTCGA GATAAACTCA AGCTAGTGTT GAATTTCCTT
    CAGAGGCTTC GATTCTTAGC AGACGAGCCC CAGCACAGCA TCCCTGATGT GTTCATATGG
    ATGATTAGCA ACAACAAACG CATCGCATAC GCCCGCATAC CCTCCAAAGA CATCCTCTAC
    TCAATTGTCG ATGAAGAGAT GGGCAAAGAC TGTGGAAAAG TTAAAGCTGT TTTTCTCAGG
    CTGCCTGGAA AGAAAGGTTT TGGGCCTGCT GGCTGGACAG TTCAGGCTAA ATTAGAGATG
    TATTTGTGGC TGGGCCTGAA TAAACAGAGG AAAGACTTCC TGATTGGCCT CCCTAGTGGC
    TTTGAAGAAA ACAAGGCAGT AAAGGGAATT GGCATACAAG CTGTCCCTCC CATCAGCCTA
    GTTTATAACA TGAAGCAGGT GTTTCAGCTG AGGGCCCACA TGTATCAAGC TCGCAGTCTG
    TTTGCTGCGG ACACTAGTGG CCTGTCTGAT CCATTTGCAA GGGTTTTCTT CTCCACCCAC
    AGCCAGGTCA CGGAGGTCCT CAGTGAGACT CTCTGTCCAA CATGGGATCA GCTGCTTGTG
    TTTGATAATG TTGAACTTTA CGGTGAGGCC GGTGAACTCC GTGATGACCC TCCAATCATT
    GTCATTGAGT TGTATGATCA AGACACTGTG GGGAAAGCTG AGTTTATTGG CCGAACATTT
    GCAAAACCAC TTACTAAGAT GGTTGATGAA CACTACGGCC CACCGCGGTT TCCTCCCCAG
    CTCGAGTATT ATCAGATCTA CCGAGGAAAC TGTGCTGCGG GAGATCTATT GGCTGCTTTT
    GAGCTTCTAC AGATTCCTTA TGACGATGAG GAGATCAGAA GAGCTCTTAT TGCTGTCCAT
    GACTTTGCTG TGCCTCAGAT CAAGATTGGT CCAGCAGGGC GGGCAGCACT TCCTCCAATC
    GATGGACCGA CCGATTCTGA CCGTGGGCCC ATTCTGCCTG TTCCATTGGG AATTCGACCA
    GTTCTCAGCA GATATCGCAT CGAGGTCCTG TTTTGGGGTC TGAGGGACCT TAAAAGGGTC
    AACCTGGCTC AGGTGGACCG ACCTCGTGTG GACATTGAGT GTGCAGGGAA AGGAGTTCAG
    TCAGCCCTAA TTCAAAACTA CAAGAAGAAT CCAAACTTCA GCACATTAGT AAAGTGGTTT
    GAAGTGGATC TGCCAGAAAA TGAGCTGCTC CATCCACCAC TTAATATTCG GGTAGTGGAT
    TGCAGGGCAT TCGGACGCTA CATTTTGGTG GGGTCTCATG CTGTGACCAC CCTTCGCAAG
    TTCATCTACA GCCCCCCAGA CAAGACTGCT AACAACTGGG CTCACACAGC TAGACTGGCC
    AATGGCTACC TGGCTCTCAG CAATGGGACG TCTCATTCCC GCCCCCAATC CCGTCCTGTT
    TCTTATCCCT CAGGTGACAT TGTGGTCAAC ATGAGCCCTG AACCTAACAT TAAGAAGATG
    GACACGGTTG TCAAGATTGA AGCTACCACC GATGCGGTTG TTAAAGTTGA CTTGAATGAG
    GATGAGAAGG AGAAAGAAAA GAAGAAGAAG AAGAAGAAGA AAGGAGAAGA AGTGGAGGAA
    GAGGAGCCGG ATGAGAGCAT GCTGGACTGG TGGTCCAAAT ACTTTGCCTC AATAGAGACT
    ATGATGGAGA ATCTAAGAGC TCAGGAGGCC GCTCAGGCAG AGGCAGAGGA AAGAGAAGAT
    TTGGAGATTG CAGCAGAGAG TGCAGAGATC AAAGCTGATG ACTTTCCTAT GAAAGGCACC
    AAACCTAAAG AGAAGAGCAA AGACAAGAAG AGCACCAAGG ACAAAAAGAA GAACAACGAT
    GGCACAGAAA AACGACCTCC AAAACCAAAA GTTGATGAAC TCATGGTGTA CAATAAAGAG
    CTGGAGAGTG AGTTCGGTAG CTTTGAGGAC TGGCTTCACA CCTTCAACCT GTACCGAGGA
    AAGGCTGGAG ATGATGATGA CCACAATGTG GTTGATGAGG ACAGAATTGT GGGCAGATTC
    AAGGGCTCCC TCTGTATGTA TAAACTACCA CTGTCTGAGG AGATCACCAG AGAGGCAGGA
    TTTGATCCAA ACATGGGCAT GTTCCAGAGT ATTCCTCACA ATGACCCTAT CAACGTTCTT
    GTCAGGATTT ATATCATTCG AGCCACAGAC CTGCATCCTG CAGATATAAA TGGAAAGGCC
    GACCCTTATA TAGTCATTAA ATTGGGAAAG TCAGACATTC GAGACAAGGA GAACTACATA
    TCCAAGCAGC TGAATCCTGT CTTTGGAAAG TCATTTGATA TAGAGGCCAC GTTCCCAATG
    GAGTCTATGC TGACTGTGGC AGTGTATGAC TGGGATTTAG TCGGAACTGA TGACCTGATT
    GGTGAGACTA AGATTGATTT GGAGAACAGA TACTACAGCA AACACAGAGC CACATGTGGC
    ATTGCTTCCA ACTACTCTGT CCATGGTTAT AATGTATGGA GAGATCCTCA GAAACCAGCT
    CAGATCCTGG CTAAGTTGTG CAAAGAAGGT AAACTGGATG GACCTCACTA TGGACCTGGG
    GGAAGAGTCA AAGTTGCAAA CCGGATCTTT CTTGGGCCGA CAGAAATTGA GGATGAGAGT
    GGTCTGAAAA AACAAACAGA AGAACACTTG GCGCTCACAG TCCTTAGACA TTGGGAGGAA
    ATCCCACGTG TTGGTTGCAA ACTCATCCCT GAGCATGTGG AGACGAGGCC TCTCCTCAAC
    CCAGACAAGC CAGGCATAGA GCAGGGAAGG ATTGAAATGT GGGTGGACAT GTTTCCTATG
    GATGTCCCAG CACCAGGACC AGCCATTGAT ATATCGCCAC GCAAACCAAA GAGATATGAG
    CTCAGGGTGA TAATATGGAA TACTGACGAG GTAATTCTGG AAGACGATGA TTACTTCACA
    GGGGAAAAGT CCAGTGACAT TTTTGTGAGG GGCTGGCTTA AAGGACAGCA GGAGGACAAA
    CAGGACACCG ATGTACATTA TCACTCTCTA ACTGGAGAGG GAAACTTCAA CTGGCGCTTT
    GTCTTCCCCT TTGACTATCT CATGGCCGAG GAGAAGATTG TTATCTCCAA GAAGGAATCA
    ATGTTTTCCT GGGATGAGAC TGAGTATAAG ATCCCTGCTC GACTCACCCT TCAAGTATGG
    GATGCCGATC ACTTTTCTGC AGATGATTTT CTGGGTGCGA TTGAGTTGGA CTTGAATAAG
    TTTCCGCGTG GGGCAAAAAC AGCCAAGCAG TGCTCTCTAG ACATGGTTCT CAAAGAGCAT
    GAGCTGCCAA CCATCTCCAT CTTCAAACAA AAGAGAGTCA AGGGCTGGTG GCCATTTGTG
    GCCCAAAATG AGAATGATGA ATTTGAGCTA ACGGGAAAAG TAGAGGCAGA GCTCCATCTG
    TTGACAGCAG AGGAGGCAGA GAAGAGTCCA GTCGGCCTGG GGAGGAATGA TCCGGAGCCA
    CTTGAGAAAC CAAATCGTCC AGACACCAGT CTAATGTGGT TCATGAACCC TCTGAGGTCC
    ATACGATACT TCATTTGGCA CAACTACCGC TGGCTGATCC TGAAGGCTCT GGCTCTGCTG
    CTGCTCCTTC TCCTCGTTGG GCTCTTCCTC TACTCTATTC CTGGATACCT GGTCAAGAAA
    CTCCTAGGGG CTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42607
    Clone ID Related Accession (Same CDS sequence) XM_009294736.3 , XM_009294735.2 , XM_009294736.2
    Accession Version XM_009294735.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6135bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product otoferlin isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009294735.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCTTTAG TGGTGCATCT GAAGACTGTC ACGGAGCTCC GTGGGAAGGG GGACCGAATC 
    GCTAAAGTCA CTTTCAGAGG TCTCTCCTTC TTCTCTCGGG TGTTGGAAAA CTGCGAGGAT
    GAAGCTCGAT TTGAGCAGGC GTTCCGATGG CCAATTGGGA GTCAAGTGGA TGGCGACGAG
    ATGCTGGAGA TCCAAGTGTT TAATTACAGC AAAGTCTTCA CTAATAGGTT AATCGGCACA
    TTCCGCATGG TTCTTCAGAA GGTGGTGGAG GAGGGACACC TGGAGGTGTC TGACACACTC
    ATTGACGACA ATAACACAGC CATACAGACA ACCATTTCCA TAGAGATCAA ATATCAGACC
    ATGGACGGGA GTGTAAAGGT GTGGAGCGAT GGTGAATTCC TTGATATTCC AGATGACCTT
    GATGGAACCT TCCAGTTTGA GACAGATAGT CTTCTCTCAG GACGCAGCCA GAGTTCTGGA
    ACTTCACCTG GACGATCCAT TCATGGCATT CCCACATTCC GCAAGGCAGG AAAAGGAGTG
    TTCTCAGCCA TGAAGCTGGG CAAGACCCGC ACCTCTAAAG ATGACCACAA AAAAGGAGAT
    GATGCTGCCA TTCTGGATGC AGAAGATCTG GATCGAAAAG CCATGCGTCT AGGCGGCATT
    CTTGACCCTG ATACAATCTC ACTGGCCTCG GTTACTGCAG TGACCACTAA TGTCTCCAAC
    AAAAGATCAA AGCCTGACAT TAAAATGGAG CCGAGTTCAG GGCGTCCGGT TGATTATCAG
    ATCAGTGTTA CAGTAATCGA GGCCCGTCAG CTGGTCGGAT TGAACATGGA CCCTGTGGTT
    TGCGTGGAAA TTGGAGAGGA GAAGAAGTAC ACGTCAATGA AGGAGTCAAC AAACTGCCCT
    TACTACAATG AGTATTTTGT CTTCGACTTC CACGTGCCTC CAGACGTCAT GTTTGACAAA
    ATTATAAAGA TTTCAGTAAT ACATTCTAAA AACCTTCTGC GAAGCGGCAC CCTCGTGGGC
    ACCTTCAAAC TAGACGTGGG GACTGTTTAC ACACAACCTG AACATCAGTT TCATCACAAA
    TGGGCGATGC TGTCAGACCC TGATGACATC ACGACTGGCT GCAAAGGTTA TGTTAAATGT
    GACATCGCAG TCGTGGGAAA AGGAGACAAC ATCAAGACTC CACACAAGGC CAGTGAAGCA
    GACGAGGATG ACATAGAGGG GAATCTTCTC TTGCCGGAGG GTGTTCCATC AGAGAGACAG
    TGGGCTCGGT TTTATGTTAA GATTTATAGA GCTGAGGGTC TGCCTAAAAT GAACACCAGT
    ATCATGGCCA ACGTGAAGAA AGCCTTTATT GGGGAAAACA GGGACCTTGT GGACCCTTAC
    GTCCTGGTGC AATTTGCAGG GCAAAAGGGC AAAACATCTG TTCAGAAGAG CAGCTACGAG
    CCCATCTGGA ACGAGCAAGT CATCTTCACG GAGATGTTCC CACCTTTGTG TAGGCGACTG
    AAAGTTCAGA TCCGTGATTC AGACAAGGTG AACGATGTTG CCATAGGAAC CCACTTCATT
    GATCTACGAA AGGTTTCCAA TGATGGAGAC AAAGGTTTCT TGCCCACTAT GGGTCCGGCT
    TGGGTGAATA TGTATGGGTC GACCCGTAAC TACACCCTGA TGGACGAACA CCAGGACCTG
    AATGAGGGGT TGGGGGAAGG GGTGTCCTTC AGAGCACGTC TGCTCATTAG TATCGCGGTG
    GAGATTCTAG ACACCACCTC TGCTGAGATC ATGAGCTCCA CTGAAGTACA CATGGAGCCG
    GTGTCCAACA TCTCAGAGAG TGCTACAGGA AAGATGGAAG AATTTTTCCT TTTCGGGTCA
    TTCTTGGAGG CCACAATGAT AGACAGAAAA ATAGGAGATA AACCCATCAG CTTTGAAGTC
    ACCATCGGTA ACTATGGTAA CCAGATTGAT GGTGTGAGCA AGCCTGCATT AGCGAAGAAG
    AAGAAGGAGG GTGGAGGAGA GAGTGAAGAA GAGGAGTCTG AGCTTATCCA CAACTCCAGC
    GAGGAAGAGG CAGAGGATGA TGGAGATCTG ACCTCTGTAC CGTCTACACC TCCTATGAAG
    CCTGTTATCA CCGACAGAAA TTACTTCCAC TTGCCTTACT TTGAAAAGAA ACCCTGCATT
    TACATCAAGA GCTGGTGGCA GGACCAGCGG AGACGTCTCT ACAACTCCAA CATAATGGAC
    AAGATAGCTG ATAAACTGGA GGAAGGTCTG AATGATGTTC AAGAGATCAT AAAAACAGAG
    AAGGCATATC CAGAACGAAG ACTAAGAGGT GTATTAGAAG AACTTAGCAC AAGCTGCAGC
    CGGTTTGTTA CACTTGCAAA CAAGGATCAG AATCTTTCTG GAAGAACCAA GCTGGACAGA
    GAGAGGCTGA AGTCCTGTAT GAGAGAAATG GAGAGTATGG GTCAGCAGGC GAAGACCATC
    CGCACACAAG TGAAAAGAAA CACAGTTCGA GATAAACTCA AGCTAGTGTT GAATTTCCTT
    CAGAGGCTTC GATTCTTAGC AGACGAGCCC CAGCACAGCA TCCCTGATGT GTTCATATGG
    ATGATTAGCA ACAACAAACG CATCGCATAC GCCCGCATAC CCTCCAAAGA CATCCTCTAC
    TCAATTGTCG ATGAAGAGAT GGGCAAAGAC TGTGGAAAAG TTAAAGCTGT TTTTCTCAGG
    CTGCCTGGAA AGAAAGGTTT TGGGCCTGCT GGCTGGACAG TTCAGGCTAA ATTAGAGATG
    TATTTGTGGC TGGGCCTGAA TAAACAGAGG AAAGACTTCC TGATTGGCCT CCCTAGTGGC
    TTTGAAGAAA ACAAGGCAGT AAAGGGAATT GGCATACAAG CTGTCCCTCC CATCAGCCTA
    GTTTATAACA TGAAGCAGGT GTTTCAGCTG AGGGCCCACA TGTATCAAGC TCGCAGTCTG
    TTTGCTGCGG ACACTAGTGG CCTGTCTGAT CCATTTGCAA GGGTTTTCTT CTCCACCCAC
    AGCCAGGTCA CGGAGGTCCT CAGTGAGACT CTCTGTCCAA CATGGGATCA GCTGCTTGTG
    TTTGATAATG TTGAACTTTA CGGTGAGGCC GGTGAACTCC GTGATGACCC TCCAATCATT
    GTCATTGAGT TGTATGATCA AGACACTGTG GGGAAAGCTG AGTTTATTGG CCGAACATTT
    GCAAAACCAC TTACTAAGAT GGTTGATGAA CACTACGGCC CACCGCGGTT TCCTCCCCAG
    CTCGAGTATT ATCAGATCTA CCGAGGAAAC TGTGCTGCGG GAGATCTATT GGCTGCTTTT
    GAGCTTCTAC AGATTCCTTA TGACGATGAG GAGATCAGAA GAGCTCTTAT TGCTGTCCAT
    GACTTTGCTG TGCCTCAGAT CAAGATTGGT CCAGCAGGGC GGGCAGCACT TCCTCCAATC
    GATGGACCGA CCGATTCTGA CCGTGGGCCC ATTCTGCCTG TTCCATTGGG AATTCGACCA
    GTTCTCAGCA GATATCGCAT CGAGGTCCTG TTTTGGGGTC TGAGGGACCT TAAAAGGGTC
    AACCTGGCTC AGGTGGACCG ACCTCGTGTG GACATTGAGT GTGCAGGGAA AGGAGTTCAG
    TCAGCCCTAA TTCAAAACTA CAAGAAGAAT CCAAACTTCA GCACATTAGT AAAGTGGTTT
    GAAGTGGATC TGCCAGAAAA TGAGCTGCTC CATCCACCAC TTAATATTCG GGTAGTGGAT
    TGCAGGGCAT TCGGACGCTA CATTTTGGTG GGGTCTCATG CTGTGACCAC CCTTCGCAAG
    TTCATCTACA GCCCCCCAGA CAAGACTGCT AACAACTGGG CTCACACAGC TAGACTGGCC
    AATGGCTACC TGGCTCTCAG CAATGGGACG TCTCATTCCC GCCCCCAATC CCGTCCTGTT
    TCTTATCCCT CAGGTGACAT TGTGGTCAAC ATGAGCCCTG AACCTAACAT TAAGAAGATG
    GACACGGTTG TCAAGATTGA AGCTACCACC GATGCGGTTG TTAAAGTTGA CTTGAATGAG
    GATGAGAAGG AGAAAGAAAA GAAGAAGAAG AAGAAGAAGA AAGGAGAAGA AGTGGAGGAA
    GAGGAGCCGG ATGAGAGCAT GCTGGACTGG TGGTCCAAAT ACTTTGCCTC AATAGAGACT
    ATGATGGAGA ATCTAAGAGC TCAGGAGGCC GCTCAGGCAG AGGCAGAGGA AAGAGAAGAT
    TTGGAGATTG CAGCAGAGAG TGCAGAGATC AAAGCTGATG ACTTTCCTAT GAAAGGCACC
    AAACCTAAAG AGAAGAGCAA AGACAAGAAG AGCACCAAGG ACAAAAAGAA GAACAACGAT
    GGCACAGAAA AACGACCTCC AAAACCAAAA GTTGATGAAC TCATGGTGTA CAATAAAGAG
    CTGGAGAGTG AGTTCGGTAG CTTTGAGGAC TGGCTTCACA CCTTCAACCT GTACCGAGGA
    AAGGCTGGAG ATGATGATGA CCACAATGTG GTTGATGAGG ACAGAATTGT GGGCAGATTC
    AAGGGCTCCC TCTGTATGTA TAAACTACCA CTGTCTGAGG AGATCACCAG AGAGGCAGGA
    TTTGATCCAA ACATGGGCAT GTTCCAGAGT ATTCCTCACA ATGACCCTAT CAACGTTCTT
    GTCAGGATTT ATATCATTCG AGCCACAGAC CTGCATCCTG CAGATATAAA TGGAAAGGCC
    GACCCTTATA TAGTCATTAA ATTGGGAAAG TCAGACATTC GAGACAAGGA GAACTACATA
    TCCAAGCAGC TGAATCCTGT CTTTGGAAAG TCATTTGATA TAGAGGCCAC GTTCCCAATG
    GAGTCTATGC TGACTGTGGC AGTGTATGAC TGGGATTTAG TCGGAACTGA TGACCTGATT
    GGTGAGACTA AGATTGATTT GGAGAACAGA TACTACAGCA AACACAGAGC CACATGTGGC
    ATTGCTTCCA ACTACTCTGT CCATGGTTAT AATGTATGGA GAGATCCTCA GAAACCAGCT
    CAGATCCTGG CTAAGTTGTG CAAAGAAGGT AAACTGGATG GACCTCACTA TGGACCTGGG
    GGAAGAGTCA AAGTTGCAAA CCGGATCTTT CTTGGGCCGA CAGAAATTGA GGATGAGAGT
    GGTCTGAAAA AACAAACAGA AGAACACTTG GCGCTCACAG TCCTTAGACA TTGGGAGGAA
    ATCCCACGTG TTGGTTGCAA ACTCATCCCT GAGCATGTGG AGACGAGGCC TCTCCTCAAC
    CCAGACAAGC CAGGCATAGA GCAGGGAAGG ATTGAAATGT GGGTGGACAT GTTTCCTATG
    GATGTCCCAG CACCAGGACC AGCCATTGAT ATATCGCCAC GCAAACCAAA GAGATATGAG
    CTCAGGGTGA TAATATGGAA TACTGACGAG GTAATTCTGG AAGACGATGA TTACTTCACA
    GGGGAAAAGT CCAGTGACAT TTTTGTGAGG GGCTGGCTTA AAGGACAGCA GGAGGACAAA
    CAGGACACCG ATGTACATTA TCACTCTCTA ACTGGAGAGG GAAACTTCAA CTGGCGCTTT
    GTCTTCCCCT TTGACTATCT CATGGCCGAG GAGAAGATTG TTATCTCCAA GAAGGAATCA
    ATGTTTTCCT GGGATGAGAC TGAGTATAAG ATCCCTGCTC GACTCACCCT TCAAGTATGG
    GATGCCGATC ACTTTTCTGC AGATGATTTT CTGGGTGCGA TTGAGTTGGA CTTGAATAAG
    TTTCCGCGTG GGGCAAAAAC AGCCAAGCAG TGCTCTCTAG ACATGGTTCT CAAAGAGCAT
    GAGCTGCCAA CCATCTCCAT CTTCAAACAA AAGAGAGTCA AGGGCTGGTG GCCATTTGTG
    GCCCAAAATG AGAATGATGA ATTTGAGCTA ACGGGAAAAG TAGAGGCAGA GCTCCATCTG
    TTGACAGCAG AGGAGGCAGA GAAGAGTCCA GTCGGCCTGG GGAGGAATGA TCCGGAGCCA
    CTTGAGAAAC CAAATCGTCC AGACACCAGT CTAATGTGGT TCATGAACCC TCTGAGGTCC
    ATACGATACT TCATTTGGCA CAACTACCGC TGGCTGATCC TGAAGGCTCT GGCTCTGCTG
    CTGCTCCTTC TCCTCGTTGG GCTCTTCCTC TACTCTATTC CTGGATACCT GGTCAAGAAA
    CTCCTAGGGG CTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009293010.1
    CDS303..6437
    Translation

    Target ORF information:

    RefSeq Version XM_009294735.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio otoferlin a (otofa), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009294735.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCTTTAG TGGTGCATCT GAAGACTGTC ACGGAGCTCC GTGGGAAGGG GGACCGAATC 
    GCTAAAGTCA CTTTCAGAGG TCTCTCCTTC TTCTCTCGGG TGTTGGAAAA CTGCGAGGAT
    GAAGCTCGAT TTGAGCAGGC GTTCCGATGG CCAATTGGGA GTCAAGTGGA TGGCGACGAG
    ATGCTGGAGA TCCAAGTGTT TAATTACAGC AAAGTCTTCA CTAATAGGTT AATCGGCACA
    TTCCGCATGG TTCTTCAGAA GGTGGTGGAG GAGGGACACC TGGAGGTGTC TGACACACTC
    ATTGACGACA ATAACACAGC CATACAGACA ACCATTTCCA TAGAGATCAA ATATCAGACC
    ATGGACGGGA GTGTAAAGGT GTGGAGCGAT GGTGAATTCC TTGATATTCC AGATGACCTT
    GATGGAACCT TCCAGTTTGA GACAGATAGT CTTCTCTCAG GACGCAGCCA GAGTTCTGGA
    ACTTCACCTG GACGATCCAT TCATGGCATT CCCACATTCC GCAAGGCAGG AAAAGGAGTG
    TTCTCAGCCA TGAAGCTGGG CAAGACCCGC ACCTCTAAAG ATGACCACAA AAAAGGAGAT
    GATGCTGCCA TTCTGGATGC AGAAGATCTG GATCGAAAAG CCATGCGTCT AGGCGGCATT
    CTTGACCCTG ATACAATCTC ACTGGCCTCG GTTACTGCAG TGACCACTAA TGTCTCCAAC
    AAAAGATCAA AGCCTGACAT TAAAATGGAG CCGAGTTCAG GGCGTCCGGT TGATTATCAG
    ATCAGTGTTA CAGTAATCGA GGCCCGTCAG CTGGTCGGAT TGAACATGGA CCCTGTGGTT
    TGCGTGGAAA TTGGAGAGGA GAAGAAGTAC ACGTCAATGA AGGAGTCAAC AAACTGCCCT
    TACTACAATG AGTATTTTGT CTTCGACTTC CACGTGCCTC CAGACGTCAT GTTTGACAAA
    ATTATAAAGA TTTCAGTAAT ACATTCTAAA AACCTTCTGC GAAGCGGCAC CCTCGTGGGC
    ACCTTCAAAC TAGACGTGGG GACTGTTTAC ACACAACCTG AACATCAGTT TCATCACAAA
    TGGGCGATGC TGTCAGACCC TGATGACATC ACGACTGGCT GCAAAGGTTA TGTTAAATGT
    GACATCGCAG TCGTGGGAAA AGGAGACAAC ATCAAGACTC CACACAAGGC CAGTGAAGCA
    GACGAGGATG ACATAGAGGG GAATCTTCTC TTGCCGGAGG GTGTTCCATC AGAGAGACAG
    TGGGCTCGGT TTTATGTTAA GATTTATAGA GCTGAGGGTC TGCCTAAAAT GAACACCAGT
    ATCATGGCCA ACGTGAAGAA AGCCTTTATT GGGGAAAACA GGGACCTTGT GGACCCTTAC
    GTCCTGGTGC AATTTGCAGG GCAAAAGGGC AAAACATCTG TTCAGAAGAG CAGCTACGAG
    CCCATCTGGA ACGAGCAAGT CATCTTCACG GAGATGTTCC CACCTTTGTG TAGGCGACTG
    AAAGTTCAGA TCCGTGATTC AGACAAGGTG AACGATGTTG CCATAGGAAC CCACTTCATT
    GATCTACGAA AGGTTTCCAA TGATGGAGAC AAAGGTTTCT TGCCCACTAT GGGTCCGGCT
    TGGGTGAATA TGTATGGGTC GACCCGTAAC TACACCCTGA TGGACGAACA CCAGGACCTG
    AATGAGGGGT TGGGGGAAGG GGTGTCCTTC AGAGCACGTC TGCTCATTAG TATCGCGGTG
    GAGATTCTAG ACACCACCTC TGCTGAGATC ATGAGCTCCA CTGAAGTACA CATGGAGCCG
    GTGTCCAACA TCTCAGAGAG TGCTACAGGA AAGATGGAAG AATTTTTCCT TTTCGGGTCA
    TTCTTGGAGG CCACAATGAT AGACAGAAAA ATAGGAGATA AACCCATCAG CTTTGAAGTC
    ACCATCGGTA ACTATGGTAA CCAGATTGAT GGTGTGAGCA AGCCTGCATT AGCGAAGAAG
    AAGAAGGAGG GTGGAGGAGA GAGTGAAGAA GAGGAGTCTG AGCTTATCCA CAACTCCAGC
    GAGGAAGAGG CAGAGGATGA TGGAGATCTG ACCTCTGTAC CGTCTACACC TCCTATGAAG
    CCTGTTATCA CCGACAGAAA TTACTTCCAC TTGCCTTACT TTGAAAAGAA ACCCTGCATT
    TACATCAAGA GCTGGTGGCA GGACCAGCGG AGACGTCTCT ACAACTCCAA CATAATGGAC
    AAGATAGCTG ATAAACTGGA GGAAGGTCTG AATGATGTTC AAGAGATCAT AAAAACAGAG
    AAGGCATATC CAGAACGAAG ACTAAGAGGT GTATTAGAAG AACTTAGCAC AAGCTGCAGC
    CGGTTTGTTA CACTTGCAAA CAAGGATCAG AATCTTTCTG GAAGAACCAA GCTGGACAGA
    GAGAGGCTGA AGTCCTGTAT GAGAGAAATG GAGAGTATGG GTCAGCAGGC GAAGACCATC
    CGCACACAAG TGAAAAGAAA CACAGTTCGA GATAAACTCA AGCTAGTGTT GAATTTCCTT
    CAGAGGCTTC GATTCTTAGC AGACGAGCCC CAGCACAGCA TCCCTGATGT GTTCATATGG
    ATGATTAGCA ACAACAAACG CATCGCATAC GCCCGCATAC CCTCCAAAGA CATCCTCTAC
    TCAATTGTCG ATGAAGAGAT GGGCAAAGAC TGTGGAAAAG TTAAAGCTGT TTTTCTCAGG
    CTGCCTGGAA AGAAAGGTTT TGGGCCTGCT GGCTGGACAG TTCAGGCTAA ATTAGAGATG
    TATTTGTGGC TGGGCCTGAA TAAACAGAGG AAAGACTTCC TGATTGGCCT CCCTAGTGGC
    TTTGAAGAAA ACAAGGCAGT AAAGGGAATT GGCATACAAG CTGTCCCTCC CATCAGCCTA
    GTTTATAACA TGAAGCAGGT GTTTCAGCTG AGGGCCCACA TGTATCAAGC TCGCAGTCTG
    TTTGCTGCGG ACACTAGTGG CCTGTCTGAT CCATTTGCAA GGGTTTTCTT CTCCACCCAC
    AGCCAGGTCA CGGAGGTCCT CAGTGAGACT CTCTGTCCAA CATGGGATCA GCTGCTTGTG
    TTTGATAATG TTGAACTTTA CGGTGAGGCC GGTGAACTCC GTGATGACCC TCCAATCATT
    GTCATTGAGT TGTATGATCA AGACACTGTG GGGAAAGCTG AGTTTATTGG CCGAACATTT
    GCAAAACCAC TTACTAAGAT GGTTGATGAA CACTACGGCC CACCGCGGTT TCCTCCCCAG
    CTCGAGTATT ATCAGATCTA CCGAGGAAAC TGTGCTGCGG GAGATCTATT GGCTGCTTTT
    GAGCTTCTAC AGATTCCTTA TGACGATGAG GAGATCAGAA GAGCTCTTAT TGCTGTCCAT
    GACTTTGCTG TGCCTCAGAT CAAGATTGGT CCAGCAGGGC GGGCAGCACT TCCTCCAATC
    GATGGACCGA CCGATTCTGA CCGTGGGCCC ATTCTGCCTG TTCCATTGGG AATTCGACCA
    GTTCTCAGCA GATATCGCAT CGAGGTCCTG TTTTGGGGTC TGAGGGACCT TAAAAGGGTC
    AACCTGGCTC AGGTGGACCG ACCTCGTGTG GACATTGAGT GTGCAGGGAA AGGAGTTCAG
    TCAGCCCTAA TTCAAAACTA CAAGAAGAAT CCAAACTTCA GCACATTAGT AAAGTGGTTT
    GAAGTGGATC TGCCAGAAAA TGAGCTGCTC CATCCACCAC TTAATATTCG GGTAGTGGAT
    TGCAGGGCAT TCGGACGCTA CATTTTGGTG GGGTCTCATG CTGTGACCAC CCTTCGCAAG
    TTCATCTACA GCCCCCCAGA CAAGACTGCT AACAACTGGG CTCACACAGC TAGACTGGCC
    AATGGCTACC TGGCTCTCAG CAATGGGACG TCTCATTCCC GCCCCCAATC CCGTCCTGTT
    TCTTATCCCT CAGGTGACAT TGTGGTCAAC ATGAGCCCTG AACCTAACAT TAAGAAGATG
    GACACGGTTG TCAAGATTGA AGCTACCACC GATGCGGTTG TTAAAGTTGA CTTGAATGAG
    GATGAGAAGG AGAAAGAAAA GAAGAAGAAG AAGAAGAAGA AAGGAGAAGA AGTGGAGGAA
    GAGGAGCCGG ATGAGAGCAT GCTGGACTGG TGGTCCAAAT ACTTTGCCTC AATAGAGACT
    ATGATGGAGA ATCTAAGAGC TCAGGAGGCC GCTCAGGCAG AGGCAGAGGA AAGAGAAGAT
    TTGGAGATTG CAGCAGAGAG TGCAGAGATC AAAGCTGATG ACTTTCCTAT GAAAGGCACC
    AAACCTAAAG AGAAGAGCAA AGACAAGAAG AGCACCAAGG ACAAAAAGAA GAACAACGAT
    GGCACAGAAA AACGACCTCC AAAACCAAAA GTTGATGAAC TCATGGTGTA CAATAAAGAG
    CTGGAGAGTG AGTTCGGTAG CTTTGAGGAC TGGCTTCACA CCTTCAACCT GTACCGAGGA
    AAGGCTGGAG ATGATGATGA CCACAATGTG GTTGATGAGG ACAGAATTGT GGGCAGATTC
    AAGGGCTCCC TCTGTATGTA TAAACTACCA CTGTCTGAGG AGATCACCAG AGAGGCAGGA
    TTTGATCCAA ACATGGGCAT GTTCCAGAGT ATTCCTCACA ATGACCCTAT CAACGTTCTT
    GTCAGGATTT ATATCATTCG AGCCACAGAC CTGCATCCTG CAGATATAAA TGGAAAGGCC
    GACCCTTATA TAGTCATTAA ATTGGGAAAG TCAGACATTC GAGACAAGGA GAACTACATA
    TCCAAGCAGC TGAATCCTGT CTTTGGAAAG TCATTTGATA TAGAGGCCAC GTTCCCAATG
    GAGTCTATGC TGACTGTGGC AGTGTATGAC TGGGATTTAG TCGGAACTGA TGACCTGATT
    GGTGAGACTA AGATTGATTT GGAGAACAGA TACTACAGCA AACACAGAGC CACATGTGGC
    ATTGCTTCCA ACTACTCTGT CCATGGTTAT AATGTATGGA GAGATCCTCA GAAACCAGCT
    CAGATCCTGG CTAAGTTGTG CAAAGAAGGT AAACTGGATG GACCTCACTA TGGACCTGGG
    GGAAGAGTCA AAGTTGCAAA CCGGATCTTT CTTGGGCCGA CAGAAATTGA GGATGAGAGT
    GGTCTGAAAA AACAAACAGA AGAACACTTG GCGCTCACAG TCCTTAGACA TTGGGAGGAA
    ATCCCACGTG TTGGTTGCAA ACTCATCCCT GAGCATGTGG AGACGAGGCC TCTCCTCAAC
    CCAGACAAGC CAGGCATAGA GCAGGGAAGG ATTGAAATGT GGGTGGACAT GTTTCCTATG
    GATGTCCCAG CACCAGGACC AGCCATTGAT ATATCGCCAC GCAAACCAAA GAGATATGAG
    CTCAGGGTGA TAATATGGAA TACTGACGAG GTAATTCTGG AAGACGATGA TTACTTCACA
    GGGGAAAAGT CCAGTGACAT TTTTGTGAGG GGCTGGCTTA AAGGACAGCA GGAGGACAAA
    CAGGACACCG ATGTACATTA TCACTCTCTA ACTGGAGAGG GAAACTTCAA CTGGCGCTTT
    GTCTTCCCCT TTGACTATCT CATGGCCGAG GAGAAGATTG TTATCTCCAA GAAGGAATCA
    ATGTTTTCCT GGGATGAGAC TGAGTATAAG ATCCCTGCTC GACTCACCCT TCAAGTATGG
    GATGCCGATC ACTTTTCTGC AGATGATTTT CTGGGTGCGA TTGAGTTGGA CTTGAATAAG
    TTTCCGCGTG GGGCAAAAAC AGCCAAGCAG TGCTCTCTAG ACATGGTTCT CAAAGAGCAT
    GAGCTGCCAA CCATCTCCAT CTTCAAACAA AAGAGAGTCA AGGGCTGGTG GCCATTTGTG
    GCCCAAAATG AGAATGATGA ATTTGAGCTA ACGGGAAAAG TAGAGGCAGA GCTCCATCTG
    TTGACAGCAG AGGAGGCAGA GAAGAGTCCA GTCGGCCTGG GGAGGAATGA TCCGGAGCCA
    CTTGAGAAAC CAAATCGTCC AGACACCAGT CTAATGTGGT TCATGAACCC TCTGAGGTCC
    ATACGATACT TCATTTGGCA CAACTACCGC TGGCTGATCC TGAAGGCTCT GGCTCTGCTG
    CTGCTCCTTC TCCTCGTTGG GCTCTTCCTC TACTCTATTC CTGGATACCT GGTCAAGAAA
    CTCCTAGGGG CTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42607
    Clone ID Related Accession (Same CDS sequence) XM_009294736.3 , XM_009294735.2 , XM_009294736.2
    Accession Version XM_009294736.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6135bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product otoferlin isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009294736.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCTTTAG TGGTGCATCT GAAGACTGTC ACGGAGCTCC GTGGGAAGGG GGACCGAATC 
    GCTAAAGTCA CTTTCAGAGG TCTCTCCTTC TTCTCTCGGG TGTTGGAAAA CTGCGAGGAT
    GAAGCTCGAT TTGAGCAGGC GTTCCGATGG CCAATTGGGA GTCAAGTGGA TGGCGACGAG
    ATGCTGGAGA TCCAAGTGTT TAATTACAGC AAAGTCTTCA CTAATAGGTT AATCGGCACA
    TTCCGCATGG TTCTTCAGAA GGTGGTGGAG GAGGGACACC TGGAGGTGTC TGACACACTC
    ATTGACGACA ATAACACAGC CATACAGACA ACCATTTCCA TAGAGATCAA ATATCAGACC
    ATGGACGGGA GTGTAAAGGT GTGGAGCGAT GGTGAATTCC TTGATATTCC AGATGACCTT
    GATGGAACCT TCCAGTTTGA GACAGATAGT CTTCTCTCAG GACGCAGCCA GAGTTCTGGA
    ACTTCACCTG GACGATCCAT TCATGGCATT CCCACATTCC GCAAGGCAGG AAAAGGAGTG
    TTCTCAGCCA TGAAGCTGGG CAAGACCCGC ACCTCTAAAG ATGACCACAA AAAAGGAGAT
    GATGCTGCCA TTCTGGATGC AGAAGATCTG GATCGAAAAG CCATGCGTCT AGGCGGCATT
    CTTGACCCTG ATACAATCTC ACTGGCCTCG GTTACTGCAG TGACCACTAA TGTCTCCAAC
    AAAAGATCAA AGCCTGACAT TAAAATGGAG CCGAGTTCAG GGCGTCCGGT TGATTATCAG
    ATCAGTGTTA CAGTAATCGA GGCCCGTCAG CTGGTCGGAT TGAACATGGA CCCTGTGGTT
    TGCGTGGAAA TTGGAGAGGA GAAGAAGTAC ACGTCAATGA AGGAGTCAAC AAACTGCCCT
    TACTACAATG AGTATTTTGT CTTCGACTTC CACGTGCCTC CAGACGTCAT GTTTGACAAA
    ATTATAAAGA TTTCAGTAAT ACATTCTAAA AACCTTCTGC GAAGCGGCAC CCTCGTGGGC
    ACCTTCAAAC TAGACGTGGG GACTGTTTAC ACACAACCTG AACATCAGTT TCATCACAAA
    TGGGCGATGC TGTCAGACCC TGATGACATC ACGACTGGCT GCAAAGGTTA TGTTAAATGT
    GACATCGCAG TCGTGGGAAA AGGAGACAAC ATCAAGACTC CACACAAGGC CAGTGAAGCA
    GACGAGGATG ACATAGAGGG GAATCTTCTC TTGCCGGAGG GTGTTCCATC AGAGAGACAG
    TGGGCTCGGT TTTATGTTAA GATTTATAGA GCTGAGGGTC TGCCTAAAAT GAACACCAGT
    ATCATGGCCA ACGTGAAGAA AGCCTTTATT GGGGAAAACA GGGACCTTGT GGACCCTTAC
    GTCCTGGTGC AATTTGCAGG GCAAAAGGGC AAAACATCTG TTCAGAAGAG CAGCTACGAG
    CCCATCTGGA ACGAGCAAGT CATCTTCACG GAGATGTTCC CACCTTTGTG TAGGCGACTG
    AAAGTTCAGA TCCGTGATTC AGACAAGGTG AACGATGTTG CCATAGGAAC CCACTTCATT
    GATCTACGAA AGGTTTCCAA TGATGGAGAC AAAGGTTTCT TGCCCACTAT GGGTCCGGCT
    TGGGTGAATA TGTATGGGTC GACCCGTAAC TACACCCTGA TGGACGAACA CCAGGACCTG
    AATGAGGGGT TGGGGGAAGG GGTGTCCTTC AGAGCACGTC TGCTCATTAG TATCGCGGTG
    GAGATTCTAG ACACCACCTC TGCTGAGATC ATGAGCTCCA CTGAAGTACA CATGGAGCCG
    GTGTCCAACA TCTCAGAGAG TGCTACAGGA AAGATGGAAG AATTTTTCCT TTTCGGGTCA
    TTCTTGGAGG CCACAATGAT AGACAGAAAA ATAGGAGATA AACCCATCAG CTTTGAAGTC
    ACCATCGGTA ACTATGGTAA CCAGATTGAT GGTGTGAGCA AGCCTGCATT AGCGAAGAAG
    AAGAAGGAGG GTGGAGGAGA GAGTGAAGAA GAGGAGTCTG AGCTTATCCA CAACTCCAGC
    GAGGAAGAGG CAGAGGATGA TGGAGATCTG ACCTCTGTAC CGTCTACACC TCCTATGAAG
    CCTGTTATCA CCGACAGAAA TTACTTCCAC TTGCCTTACT TTGAAAAGAA ACCCTGCATT
    TACATCAAGA GCTGGTGGCA GGACCAGCGG AGACGTCTCT ACAACTCCAA CATAATGGAC
    AAGATAGCTG ATAAACTGGA GGAAGGTCTG AATGATGTTC AAGAGATCAT AAAAACAGAG
    AAGGCATATC CAGAACGAAG ACTAAGAGGT GTATTAGAAG AACTTAGCAC AAGCTGCAGC
    CGGTTTGTTA CACTTGCAAA CAAGGATCAG AATCTTTCTG GAAGAACCAA GCTGGACAGA
    GAGAGGCTGA AGTCCTGTAT GAGAGAAATG GAGAGTATGG GTCAGCAGGC GAAGACCATC
    CGCACACAAG TGAAAAGAAA CACAGTTCGA GATAAACTCA AGCTAGTGTT GAATTTCCTT
    CAGAGGCTTC GATTCTTAGC AGACGAGCCC CAGCACAGCA TCCCTGATGT GTTCATATGG
    ATGATTAGCA ACAACAAACG CATCGCATAC GCCCGCATAC CCTCCAAAGA CATCCTCTAC
    TCAATTGTCG ATGAAGAGAT GGGCAAAGAC TGTGGAAAAG TTAAAGCTGT TTTTCTCAGG
    CTGCCTGGAA AGAAAGGTTT TGGGCCTGCT GGCTGGACAG TTCAGGCTAA ATTAGAGATG
    TATTTGTGGC TGGGCCTGAA TAAACAGAGG AAAGACTTCC TGATTGGCCT CCCTAGTGGC
    TTTGAAGAAA ACAAGGCAGT AAAGGGAATT GGCATACAAG CTGTCCCTCC CATCAGCCTA
    GTTTATAACA TGAAGCAGGT GTTTCAGCTG AGGGCCCACA TGTATCAAGC TCGCAGTCTG
    TTTGCTGCGG ACACTAGTGG CCTGTCTGAT CCATTTGCAA GGGTTTTCTT CTCCACCCAC
    AGCCAGGTCA CGGAGGTCCT CAGTGAGACT CTCTGTCCAA CATGGGATCA GCTGCTTGTG
    TTTGATAATG TTGAACTTTA CGGTGAGGCC GGTGAACTCC GTGATGACCC TCCAATCATT
    GTCATTGAGT TGTATGATCA AGACACTGTG GGGAAAGCTG AGTTTATTGG CCGAACATTT
    GCAAAACCAC TTACTAAGAT GGTTGATGAA CACTACGGCC CACCGCGGTT TCCTCCCCAG
    CTCGAGTATT ATCAGATCTA CCGAGGAAAC TGTGCTGCGG GAGATCTATT GGCTGCTTTT
    GAGCTTCTAC AGATTCCTTA TGACGATGAG GAGATCAGAA GAGCTCTTAT TGCTGTCCAT
    GACTTTGCTG TGCCTCAGAT CAAGATTGGT CCAGCAGGGC GGGCAGCACT TCCTCCAATC
    GATGGACCGA CCGATTCTGA CCGTGGGCCC ATTCTGCCTG TTCCATTGGG AATTCGACCA
    GTTCTCAGCA GATATCGCAT CGAGGTCCTG TTTTGGGGTC TGAGGGACCT TAAAAGGGTC
    AACCTGGCTC AGGTGGACCG ACCTCGTGTG GACATTGAGT GTGCAGGGAA AGGAGTTCAG
    TCAGCCCTAA TTCAAAACTA CAAGAAGAAT CCAAACTTCA GCACATTAGT AAAGTGGTTT
    GAAGTGGATC TGCCAGAAAA TGAGCTGCTC CATCCACCAC TTAATATTCG GGTAGTGGAT
    TGCAGGGCAT TCGGACGCTA CATTTTGGTG GGGTCTCATG CTGTGACCAC CCTTCGCAAG
    TTCATCTACA GCCCCCCAGA CAAGACTGCT AACAACTGGG CTCACACAGC TAGACTGGCC
    AATGGCTACC TGGCTCTCAG CAATGGGACG TCTCATTCCC GCCCCCAATC CCGTCCTGTT
    TCTTATCCCT CAGGTGACAT TGTGGTCAAC ATGAGCCCTG AACCTAACAT TAAGAAGATG
    GACACGGTTG TCAAGATTGA AGCTACCACC GATGCGGTTG TTAAAGTTGA CTTGAATGAG
    GATGAGAAGG AGAAAGAAAA GAAGAAGAAG AAGAAGAAGA AAGGAGAAGA AGTGGAGGAA
    GAGGAGCCGG ATGAGAGCAT GCTGGACTGG TGGTCCAAAT ACTTTGCCTC AATAGAGACT
    ATGATGGAGA ATCTAAGAGC TCAGGAGGCC GCTCAGGCAG AGGCAGAGGA AAGAGAAGAT
    TTGGAGATTG CAGCAGAGAG TGCAGAGATC AAAGCTGATG ACTTTCCTAT GAAAGGCACC
    AAACCTAAAG AGAAGAGCAA AGACAAGAAG AGCACCAAGG ACAAAAAGAA GAACAACGAT
    GGCACAGAAA AACGACCTCC AAAACCAAAA GTTGATGAAC TCATGGTGTA CAATAAAGAG
    CTGGAGAGTG AGTTCGGTAG CTTTGAGGAC TGGCTTCACA CCTTCAACCT GTACCGAGGA
    AAGGCTGGAG ATGATGATGA CCACAATGTG GTTGATGAGG ACAGAATTGT GGGCAGATTC
    AAGGGCTCCC TCTGTATGTA TAAACTACCA CTGTCTGAGG AGATCACCAG AGAGGCAGGA
    TTTGATCCAA ACATGGGCAT GTTCCAGAGT ATTCCTCACA ATGACCCTAT CAACGTTCTT
    GTCAGGATTT ATATCATTCG AGCCACAGAC CTGCATCCTG CAGATATAAA TGGAAAGGCC
    GACCCTTATA TAGTCATTAA ATTGGGAAAG TCAGACATTC GAGACAAGGA GAACTACATA
    TCCAAGCAGC TGAATCCTGT CTTTGGAAAG TCATTTGATA TAGAGGCCAC GTTCCCAATG
    GAGTCTATGC TGACTGTGGC AGTGTATGAC TGGGATTTAG TCGGAACTGA TGACCTGATT
    GGTGAGACTA AGATTGATTT GGAGAACAGA TACTACAGCA AACACAGAGC CACATGTGGC
    ATTGCTTCCA ACTACTCTGT CCATGGTTAT AATGTATGGA GAGATCCTCA GAAACCAGCT
    CAGATCCTGG CTAAGTTGTG CAAAGAAGGT AAACTGGATG GACCTCACTA TGGACCTGGG
    GGAAGAGTCA AAGTTGCAAA CCGGATCTTT CTTGGGCCGA CAGAAATTGA GGATGAGAGT
    GGTCTGAAAA AACAAACAGA AGAACACTTG GCGCTCACAG TCCTTAGACA TTGGGAGGAA
    ATCCCACGTG TTGGTTGCAA ACTCATCCCT GAGCATGTGG AGACGAGGCC TCTCCTCAAC
    CCAGACAAGC CAGGCATAGA GCAGGGAAGG ATTGAAATGT GGGTGGACAT GTTTCCTATG
    GATGTCCCAG CACCAGGACC AGCCATTGAT ATATCGCCAC GCAAACCAAA GAGATATGAG
    CTCAGGGTGA TAATATGGAA TACTGACGAG GTAATTCTGG AAGACGATGA TTACTTCACA
    GGGGAAAAGT CCAGTGACAT TTTTGTGAGG GGCTGGCTTA AAGGACAGCA GGAGGACAAA
    CAGGACACCG ATGTACATTA TCACTCTCTA ACTGGAGAGG GAAACTTCAA CTGGCGCTTT
    GTCTTCCCCT TTGACTATCT CATGGCCGAG GAGAAGATTG TTATCTCCAA GAAGGAATCA
    ATGTTTTCCT GGGATGAGAC TGAGTATAAG ATCCCTGCTC GACTCACCCT TCAAGTATGG
    GATGCCGATC ACTTTTCTGC AGATGATTTT CTGGGTGCGA TTGAGTTGGA CTTGAATAAG
    TTTCCGCGTG GGGCAAAAAC AGCCAAGCAG TGCTCTCTAG ACATGGTTCT CAAAGAGCAT
    GAGCTGCCAA CCATCTCCAT CTTCAAACAA AAGAGAGTCA AGGGCTGGTG GCCATTTGTG
    GCCCAAAATG AGAATGATGA ATTTGAGCTA ACGGGAAAAG TAGAGGCAGA GCTCCATCTG
    TTGACAGCAG AGGAGGCAGA GAAGAGTCCA GTCGGCCTGG GGAGGAATGA TCCGGAGCCA
    CTTGAGAAAC CAAATCGTCC AGACACCAGT CTAATGTGGT TCATGAACCC TCTGAGGTCC
    ATACGATACT TCATTTGGCA CAACTACCGC TGGCTGATCC TGAAGGCTCT GGCTCTGCTG
    CTGCTCCTTC TCCTCGTTGG GCTCTTCCTC TACTCTATTC CTGGATACCT GGTCAAGAAA
    CTCCTAGGGG CTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009293011.1
    CDS303..6437
    Translation

    Target ORF information:

    RefSeq Version XM_009294736.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio otoferlin a (otofa), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009294736.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCTTTAG TGGTGCATCT GAAGACTGTC ACGGAGCTCC GTGGGAAGGG GGACCGAATC 
    GCTAAAGTCA CTTTCAGAGG TCTCTCCTTC TTCTCTCGGG TGTTGGAAAA CTGCGAGGAT
    GAAGCTCGAT TTGAGCAGGC GTTCCGATGG CCAATTGGGA GTCAAGTGGA TGGCGACGAG
    ATGCTGGAGA TCCAAGTGTT TAATTACAGC AAAGTCTTCA CTAATAGGTT AATCGGCACA
    TTCCGCATGG TTCTTCAGAA GGTGGTGGAG GAGGGACACC TGGAGGTGTC TGACACACTC
    ATTGACGACA ATAACACAGC CATACAGACA ACCATTTCCA TAGAGATCAA ATATCAGACC
    ATGGACGGGA GTGTAAAGGT GTGGAGCGAT GGTGAATTCC TTGATATTCC AGATGACCTT
    GATGGAACCT TCCAGTTTGA GACAGATAGT CTTCTCTCAG GACGCAGCCA GAGTTCTGGA
    ACTTCACCTG GACGATCCAT TCATGGCATT CCCACATTCC GCAAGGCAGG AAAAGGAGTG
    TTCTCAGCCA TGAAGCTGGG CAAGACCCGC ACCTCTAAAG ATGACCACAA AAAAGGAGAT
    GATGCTGCCA TTCTGGATGC AGAAGATCTG GATCGAAAAG CCATGCGTCT AGGCGGCATT
    CTTGACCCTG ATACAATCTC ACTGGCCTCG GTTACTGCAG TGACCACTAA TGTCTCCAAC
    AAAAGATCAA AGCCTGACAT TAAAATGGAG CCGAGTTCAG GGCGTCCGGT TGATTATCAG
    ATCAGTGTTA CAGTAATCGA GGCCCGTCAG CTGGTCGGAT TGAACATGGA CCCTGTGGTT
    TGCGTGGAAA TTGGAGAGGA GAAGAAGTAC ACGTCAATGA AGGAGTCAAC AAACTGCCCT
    TACTACAATG AGTATTTTGT CTTCGACTTC CACGTGCCTC CAGACGTCAT GTTTGACAAA
    ATTATAAAGA TTTCAGTAAT ACATTCTAAA AACCTTCTGC GAAGCGGCAC CCTCGTGGGC
    ACCTTCAAAC TAGACGTGGG GACTGTTTAC ACACAACCTG AACATCAGTT TCATCACAAA
    TGGGCGATGC TGTCAGACCC TGATGACATC ACGACTGGCT GCAAAGGTTA TGTTAAATGT
    GACATCGCAG TCGTGGGAAA AGGAGACAAC ATCAAGACTC CACACAAGGC CAGTGAAGCA
    GACGAGGATG ACATAGAGGG GAATCTTCTC TTGCCGGAGG GTGTTCCATC AGAGAGACAG
    TGGGCTCGGT TTTATGTTAA GATTTATAGA GCTGAGGGTC TGCCTAAAAT GAACACCAGT
    ATCATGGCCA ACGTGAAGAA AGCCTTTATT GGGGAAAACA GGGACCTTGT GGACCCTTAC
    GTCCTGGTGC AATTTGCAGG GCAAAAGGGC AAAACATCTG TTCAGAAGAG CAGCTACGAG
    CCCATCTGGA ACGAGCAAGT CATCTTCACG GAGATGTTCC CACCTTTGTG TAGGCGACTG
    AAAGTTCAGA TCCGTGATTC AGACAAGGTG AACGATGTTG CCATAGGAAC CCACTTCATT
    GATCTACGAA AGGTTTCCAA TGATGGAGAC AAAGGTTTCT TGCCCACTAT GGGTCCGGCT
    TGGGTGAATA TGTATGGGTC GACCCGTAAC TACACCCTGA TGGACGAACA CCAGGACCTG
    AATGAGGGGT TGGGGGAAGG GGTGTCCTTC AGAGCACGTC TGCTCATTAG TATCGCGGTG
    GAGATTCTAG ACACCACCTC TGCTGAGATC ATGAGCTCCA CTGAAGTACA CATGGAGCCG
    GTGTCCAACA TCTCAGAGAG TGCTACAGGA AAGATGGAAG AATTTTTCCT TTTCGGGTCA
    TTCTTGGAGG CCACAATGAT AGACAGAAAA ATAGGAGATA AACCCATCAG CTTTGAAGTC
    ACCATCGGTA ACTATGGTAA CCAGATTGAT GGTGTGAGCA AGCCTGCATT AGCGAAGAAG
    AAGAAGGAGG GTGGAGGAGA GAGTGAAGAA GAGGAGTCTG AGCTTATCCA CAACTCCAGC
    GAGGAAGAGG CAGAGGATGA TGGAGATCTG ACCTCTGTAC CGTCTACACC TCCTATGAAG
    CCTGTTATCA CCGACAGAAA TTACTTCCAC TTGCCTTACT TTGAAAAGAA ACCCTGCATT
    TACATCAAGA GCTGGTGGCA GGACCAGCGG AGACGTCTCT ACAACTCCAA CATAATGGAC
    AAGATAGCTG ATAAACTGGA GGAAGGTCTG AATGATGTTC AAGAGATCAT AAAAACAGAG
    AAGGCATATC CAGAACGAAG ACTAAGAGGT GTATTAGAAG AACTTAGCAC AAGCTGCAGC
    CGGTTTGTTA CACTTGCAAA CAAGGATCAG AATCTTTCTG GAAGAACCAA GCTGGACAGA
    GAGAGGCTGA AGTCCTGTAT GAGAGAAATG GAGAGTATGG GTCAGCAGGC GAAGACCATC
    CGCACACAAG TGAAAAGAAA CACAGTTCGA GATAAACTCA AGCTAGTGTT GAATTTCCTT
    CAGAGGCTTC GATTCTTAGC AGACGAGCCC CAGCACAGCA TCCCTGATGT GTTCATATGG
    ATGATTAGCA ACAACAAACG CATCGCATAC GCCCGCATAC CCTCCAAAGA CATCCTCTAC
    TCAATTGTCG ATGAAGAGAT GGGCAAAGAC TGTGGAAAAG TTAAAGCTGT TTTTCTCAGG
    CTGCCTGGAA AGAAAGGTTT TGGGCCTGCT GGCTGGACAG TTCAGGCTAA ATTAGAGATG
    TATTTGTGGC TGGGCCTGAA TAAACAGAGG AAAGACTTCC TGATTGGCCT CCCTAGTGGC
    TTTGAAGAAA ACAAGGCAGT AAAGGGAATT GGCATACAAG CTGTCCCTCC CATCAGCCTA
    GTTTATAACA TGAAGCAGGT GTTTCAGCTG AGGGCCCACA TGTATCAAGC TCGCAGTCTG
    TTTGCTGCGG ACACTAGTGG CCTGTCTGAT CCATTTGCAA GGGTTTTCTT CTCCACCCAC
    AGCCAGGTCA CGGAGGTCCT CAGTGAGACT CTCTGTCCAA CATGGGATCA GCTGCTTGTG
    TTTGATAATG TTGAACTTTA CGGTGAGGCC GGTGAACTCC GTGATGACCC TCCAATCATT
    GTCATTGAGT TGTATGATCA AGACACTGTG GGGAAAGCTG AGTTTATTGG CCGAACATTT
    GCAAAACCAC TTACTAAGAT GGTTGATGAA CACTACGGCC CACCGCGGTT TCCTCCCCAG
    CTCGAGTATT ATCAGATCTA CCGAGGAAAC TGTGCTGCGG GAGATCTATT GGCTGCTTTT
    GAGCTTCTAC AGATTCCTTA TGACGATGAG GAGATCAGAA GAGCTCTTAT TGCTGTCCAT
    GACTTTGCTG TGCCTCAGAT CAAGATTGGT CCAGCAGGGC GGGCAGCACT TCCTCCAATC
    GATGGACCGA CCGATTCTGA CCGTGGGCCC ATTCTGCCTG TTCCATTGGG AATTCGACCA
    GTTCTCAGCA GATATCGCAT CGAGGTCCTG TTTTGGGGTC TGAGGGACCT TAAAAGGGTC
    AACCTGGCTC AGGTGGACCG ACCTCGTGTG GACATTGAGT GTGCAGGGAA AGGAGTTCAG
    TCAGCCCTAA TTCAAAACTA CAAGAAGAAT CCAAACTTCA GCACATTAGT AAAGTGGTTT
    GAAGTGGATC TGCCAGAAAA TGAGCTGCTC CATCCACCAC TTAATATTCG GGTAGTGGAT
    TGCAGGGCAT TCGGACGCTA CATTTTGGTG GGGTCTCATG CTGTGACCAC CCTTCGCAAG
    TTCATCTACA GCCCCCCAGA CAAGACTGCT AACAACTGGG CTCACACAGC TAGACTGGCC
    AATGGCTACC TGGCTCTCAG CAATGGGACG TCTCATTCCC GCCCCCAATC CCGTCCTGTT
    TCTTATCCCT CAGGTGACAT TGTGGTCAAC ATGAGCCCTG AACCTAACAT TAAGAAGATG
    GACACGGTTG TCAAGATTGA AGCTACCACC GATGCGGTTG TTAAAGTTGA CTTGAATGAG
    GATGAGAAGG AGAAAGAAAA GAAGAAGAAG AAGAAGAAGA AAGGAGAAGA AGTGGAGGAA
    GAGGAGCCGG ATGAGAGCAT GCTGGACTGG TGGTCCAAAT ACTTTGCCTC AATAGAGACT
    ATGATGGAGA ATCTAAGAGC TCAGGAGGCC GCTCAGGCAG AGGCAGAGGA AAGAGAAGAT
    TTGGAGATTG CAGCAGAGAG TGCAGAGATC AAAGCTGATG ACTTTCCTAT GAAAGGCACC
    AAACCTAAAG AGAAGAGCAA AGACAAGAAG AGCACCAAGG ACAAAAAGAA GAACAACGAT
    GGCACAGAAA AACGACCTCC AAAACCAAAA GTTGATGAAC TCATGGTGTA CAATAAAGAG
    CTGGAGAGTG AGTTCGGTAG CTTTGAGGAC TGGCTTCACA CCTTCAACCT GTACCGAGGA
    AAGGCTGGAG ATGATGATGA CCACAATGTG GTTGATGAGG ACAGAATTGT GGGCAGATTC
    AAGGGCTCCC TCTGTATGTA TAAACTACCA CTGTCTGAGG AGATCACCAG AGAGGCAGGA
    TTTGATCCAA ACATGGGCAT GTTCCAGAGT ATTCCTCACA ATGACCCTAT CAACGTTCTT
    GTCAGGATTT ATATCATTCG AGCCACAGAC CTGCATCCTG CAGATATAAA TGGAAAGGCC
    GACCCTTATA TAGTCATTAA ATTGGGAAAG TCAGACATTC GAGACAAGGA GAACTACATA
    TCCAAGCAGC TGAATCCTGT CTTTGGAAAG TCATTTGATA TAGAGGCCAC GTTCCCAATG
    GAGTCTATGC TGACTGTGGC AGTGTATGAC TGGGATTTAG TCGGAACTGA TGACCTGATT
    GGTGAGACTA AGATTGATTT GGAGAACAGA TACTACAGCA AACACAGAGC CACATGTGGC
    ATTGCTTCCA ACTACTCTGT CCATGGTTAT AATGTATGGA GAGATCCTCA GAAACCAGCT
    CAGATCCTGG CTAAGTTGTG CAAAGAAGGT AAACTGGATG GACCTCACTA TGGACCTGGG
    GGAAGAGTCA AAGTTGCAAA CCGGATCTTT CTTGGGCCGA CAGAAATTGA GGATGAGAGT
    GGTCTGAAAA AACAAACAGA AGAACACTTG GCGCTCACAG TCCTTAGACA TTGGGAGGAA
    ATCCCACGTG TTGGTTGCAA ACTCATCCCT GAGCATGTGG AGACGAGGCC TCTCCTCAAC
    CCAGACAAGC CAGGCATAGA GCAGGGAAGG ATTGAAATGT GGGTGGACAT GTTTCCTATG
    GATGTCCCAG CACCAGGACC AGCCATTGAT ATATCGCCAC GCAAACCAAA GAGATATGAG
    CTCAGGGTGA TAATATGGAA TACTGACGAG GTAATTCTGG AAGACGATGA TTACTTCACA
    GGGGAAAAGT CCAGTGACAT TTTTGTGAGG GGCTGGCTTA AAGGACAGCA GGAGGACAAA
    CAGGACACCG ATGTACATTA TCACTCTCTA ACTGGAGAGG GAAACTTCAA CTGGCGCTTT
    GTCTTCCCCT TTGACTATCT CATGGCCGAG GAGAAGATTG TTATCTCCAA GAAGGAATCA
    ATGTTTTCCT GGGATGAGAC TGAGTATAAG ATCCCTGCTC GACTCACCCT TCAAGTATGG
    GATGCCGATC ACTTTTCTGC AGATGATTTT CTGGGTGCGA TTGAGTTGGA CTTGAATAAG
    TTTCCGCGTG GGGCAAAAAC AGCCAAGCAG TGCTCTCTAG ACATGGTTCT CAAAGAGCAT
    GAGCTGCCAA CCATCTCCAT CTTCAAACAA AAGAGAGTCA AGGGCTGGTG GCCATTTGTG
    GCCCAAAATG AGAATGATGA ATTTGAGCTA ACGGGAAAAG TAGAGGCAGA GCTCCATCTG
    TTGACAGCAG AGGAGGCAGA GAAGAGTCCA GTCGGCCTGG GGAGGAATGA TCCGGAGCCA
    CTTGAGAAAC CAAATCGTCC AGACACCAGT CTAATGTGGT TCATGAACCC TCTGAGGTCC
    ATACGATACT TCATTTGGCA CAACTACCGC TGGCTGATCC TGAAGGCTCT GGCTCTGCTG
    CTGCTCCTTC TCCTCGTTGG GCTCTTCCTC TACTCTATTC CTGGATACCT GGTCAAGAAA
    CTCCTAGGGG CTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42608
    Clone ID Related Accession (Same CDS sequence) XM_021468441.1
    Accession Version XM_021468441.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6111bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product otoferlin isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGGCTTTAG TGGTGCATCT GAAGACTGTC ACGGAGCTCC GTGGGAAGGG GGACCGAATC 
    GCTAAAGTCA CTTTCAGAGG TCTCTCCTTC TTCTCTCGGG TGTTGGAAAA CTGCGAGGAT
    GAAGCTCGAT TTGAGCAGGC GTTCCGATGG CCAATTGGGA GTCAAGTGGA TGGCGACGAG
    ATGCTGGAGA TCCAAGTGTT TAATTACAGC AAAGTCTTCA CTAATAGGTT AATCGGCACA
    TTCCGCATGG TTCTTCAGAA GGTGGTGGAG GAGGGACACC TGGAGGTGTC TGACACACTC
    ATTGACGACA ATAACACAGC CATACAGACA ACCATTTCCA TAGAGATCAA ATATCAGACC
    ATGGACGGGA GTGTAAAGGT GTGGAGCGAT GGTGAATTCC TTGATATTCC AGATGACCTT
    GATGGAACCT TCCAGTTTGA GACAGATAGT CTTCTCTCAG GACGCAGCCA GAGTTCTGGA
    ACTTCACCTG GACGATCCAT TCATGGCATT CCCACATTCC GCAAGGCAGG AAAAGGAGTG
    TTCTCAGCCA TGAAGCTGGG CAAGACCCGC ACCTCTAAAG ATGACCACAA AAAAGGAGAT
    GATGCTGCCA TTCTGGATGC AGAAGATCTG GATCGAAAAG CCATGCGTCT AGGCGGCATT
    CTTGACCCTG ATACAATCTC ACTGGCCTCG GTTACTGCAG TGACCACTAA TGTCTCCAAC
    AAAAGATCAA AGCCTGACAT TAAAATGGAG CCGAGTTCAG GGCGTCCGGT TGATTATCAG
    ATCAGTGTTA CAGTAATCGA GGCCCGTCAG CTGGTCGGAT TGAACATGGA CCCTGTGGTT
    TGCGTGGAAA TTGGAGAGGA GAAGAAGTAC ACGTCAATGA AGGAGTCAAC AAACTGCCCT
    TACTACAATG AGTATTTTGT CTTCGACTTC CACGTGCCTC CAGACGTCAT GTTTGACAAA
    ATTATAAAGA TTTCAGTAAT ACATTCTAAA AACCTTCTGC GAAGCGGCAC CCTCGTGGGC
    ACCTTCAAAC TAGACGTGGG GACTGTTTAC ACACAACCTG AACATCAGTT TCATCACAAA
    TGGGCGATGC TGTCAGACCC TGATGACATC ACGACTGGCT GCAAAGGTTA TGTTAAATGT
    GACATCGCAG TCGTGGGAAA AGGAGACAAC ATCAAGACTC CACACAAGGC CAGTGAAGCA
    GACGAGGATG ACATAGAGGG GAATCTTCTC TTGCCGGAGG GTGTTCCATC AGAGAGACAG
    TGGGCTCGGT TTTATGTTAA GATTTATAGA GCTGAGGGTC TGCCTAAAAT GAACACCAGT
    ATCATGGCCA ACGTGAAGAA AGCCTTTATT GGGGAAAACA GGGACCTTGT GGACCCTTAC
    GTCCTGGTGC AATTTGCAGG GCAAAAGGGC AAAACATCTG TTCAGAAGAG CAGCTACGAG
    CCCATCTGGA ACGAGCAAGT CATCTTCACG GAGATGTTCC CACCTTTGTG TAGGCGACTG
    AAAGTTCAGA TCCGTGATTC AGACAAGGTG AACGATGTTG CCATAGGAAC CCACTTCATT
    GATCTACGAA AGGTTTCCAA TGATGGAGAC AAAGGTTTCT TGCCCACTAT GGGTCCGGCT
    TGGGTGAATA TGTATGGGTC GACCCGTAAC TACACCCTGA TGGACGAACA CCAGGACCTG
    AATGAGGGGT TGGGGGAAGG GGTGTCCTTC AGAGCACGTC TGCTCATTAG TATCGCGGTG
    GAGATTCTAG ACACCACCTC TGCTGAGATC ATGAGCTCCA CTGAAGTACA CATGGAGCCG
    GTGTCCAACA TCTCAGAGAG TGCTACAGGA AAGATGGAAG AATTTTTCCT TTTCGGGTCA
    TTCTTGGAGG CCACAATGAT AGACAGAAAA ATAGGAGATA AACCCATCAG CTTTGAAGTC
    ACCATCGGTA ACTATGGTAA CCAGATTGAT GGTGTGAGCA AGCCTGCATT AGCGAAGAAG
    AAGAAGGAGG GTGGAGGAGA GAGTGAAGAA GAGGAGTCTG AGCTTATCCA CAACTCCAGC
    GAGGAAGAGG CAGAGGATGA TGGAGATCTG ACCTCTGTAC CGTCTACACC TCCTATGAAG
    CCTGTTATCA CCGACAGAAA TTACTTCCAC TTGCCTTACT TTGAAAAGAA ACCCTGCATT
    TACATCAAGA GCTGGTGGCA GGACCAGCGG AGACGTCTCT ACAACTCCAA CATAATGGAC
    AAGATAGCTG ATAAACTGGA GGAAGGTCTG AATGATGTTC AAGAGATCAT AAAAACAGAG
    AAGGCATATC CAGAACGAAG ACTAAGAGGT GTATTAGAAG AACTTAGCAC AAGCTGCAGC
    CGGTTTGTTA CACTTGCAAA CAAGGATCAG AATCTTTCTG GAAGAACCAA GCTGGACAGA
    GAGAGGCTGA AGTCCTGTAT GAGAGAAATG GAGAGTATGG GTCAGCAGGC GAAGACCATC
    CGCACACAAG TGAAAAGAAA CACAGTTCGA GATAAACTCA AGCTAGTGTT GAATTTCCTT
    CAGAGGCTTC GATTCTTAGC AGACGAGCCC CAGCACAGCA TCCCTGATGT GTTCATATGG
    ATGATTAGCA ACAACAAACG CATCGCATAC GCCCGCATAC CCTCCAAAGA CATCCTCTAC
    TCAATTGTCG ATGAAGAGAT GGGCAAAGAC TGTGGAAAAG TTAAAGCTGT TTTTCTCAGG
    CTGCCTGGAA AGAAAGGTTT TGGGCCTGCT GGCTGGACAG TTCAGGCTAA ATTAGAGATG
    TATTTGTGGC TGGGCCTGAA TAAACAGAGG AAAGACTTCC TGATTGGCCT CCCTAGTGGC
    TTTGAAGAAA ACAAGGCAGT AAAGGGAATT GGCATACAAG CTGTCCCTCC CATCAGCCTA
    GTTTATAACA TGAAGCAGGT GTTTCAGCTG AGGGCCCACA TGTATCAAGC TCGCAGTCTG
    TTTGCTGCGG ACACTAGTGG CCTGTCTGAT CCATTTGCAA GGGTTTTCTT CTCCACCCAC
    AGCCAGGTCA CGGAGGTCCT CAGTGAGACT CTCTGTCCAA CATGGGATCA GCTGCTTGTG
    TTTGATAATG TTGAACTTTA CGGTGAGGCC GGTGAACTCC GTGATGACCC TCCAATCATT
    GTCATTGAGT TGTATGATCA AGACACTGTG GGGAAAGCTG AGTTTATTGG CCGAACATTT
    GCAAAACCAC TTACTAAGAT GGTTGATGAA CACTACGGCC CACCGCGGTT TCCTCCCCAG
    CTCGAGTATT ATCAGATCTA CCGAGGAAAC TGTGCTGCGG GAGATCTATT GGCTGCTTTT
    GAGCTTCTAC AGATTCCTTA TGACGATGAG GAGATCAGAA GAGCTCTTAT TGCTGTCCAT
    GACTTTGCTG TGCCTCAGAT CAAGATTGGT CCAGCAGGGC GGGCAGCACT TCCTCCAATC
    GATGGACCGA CCGATTCTGA CCGTGGGCCC ATTCTGCCTG TTCCATTGGG AATTCGACCA
    GTTCTCAGCA GATATCGCAT CGAGGTCCTG TTTTGGGGTC TGAGGGACCT TAAAAGGGTC
    AACCTGGCTC AGGTGGACCG ACCTCGTGTG GACATTGAGT GTGCAGGGAA AGGAGTTCAG
    TCAGCCCTAA TTCAAAACTA CAAGAAGAAT CCAAACTTCA GCACATTAGT AAAGTGGTTT
    GAAGTGGATC TGCCAGAAAA TGAGCTGCTC CATCCACCAC TTAATATTCG GGTAGTGGAT
    TGCAGGGCAT TCGGACGCTA CATTTTGGTG GGGTCTCATG CTGTGACCAC CCTTCGCAAG
    TTCATCTACA GCCCCCCAGA CAAGACTGCT AACAACTGGG CTCACACAGC TAGACTGGCC
    AATGGCTACC TGGCTCTCAG CAATGGGACG TCTCATTCCC GCCCCCAATC CCGTCCTGTT
    TCTTATCCCT CAGGTGACAT TGTGGTCAAC ATGAGCCCTG AACCTAACAT TAAGAAGATG
    GACACGGTTG TCAAGATTGA AGCTAATGAG GATGAGAAGG AGAAAGAAAA GAAGAAGAAG
    AAGAAGAAGA AAGGAGAAGA AGTGGAGGAA GAGGAGCCGG ATGAGAGCAT GCTGGACTGG
    TGGTCCAAAT ACTTTGCCTC AATAGAGACT ATGATGGAGA ATCTAAGAGC TCAGGAGGCC
    GCTCAGGCAG AGGCAGAGGA AAGAGAAGAT TTGGAGATTG CAGCAGAGAG TGCAGAGATC
    AAAGCTGATG ACTTTCCTAT GAAAGGCACC AAACCTAAAG AGAAGAGCAA AGACAAGAAG
    AGCACCAAGG ACAAAAAGAA GAACAACGAT GGCACAGAAA AACGACCTCC AAAACCAAAA
    GTTGATGAAC TCATGGTGTA CAATAAAGAG CTGGAGAGTG AGTTCGGTAG CTTTGAGGAC
    TGGCTTCACA CCTTCAACCT GTACCGAGGA AAGGCTGGAG ATGATGATGA CCACAATGTG
    GTTGATGAGG ACAGAATTGT GGGCAGATTC AAGGGCTCCC TCTGTATGTA TAAACTACCA
    CTGTCTGAGG AGATCACCAG AGAGGCAGGA TTTGATCCAA ACATGGGCAT GTTCCAGAGT
    ATTCCTCACA ATGACCCTAT CAACGTTCTT GTCAGGATTT ATATCATTCG AGCCACAGAC
    CTGCATCCTG CAGATATAAA TGGAAAGGCC GACCCTTATA TAGTCATTAA ATTGGGAAAG
    TCAGACATTC GAGACAAGGA GAACTACATA TCCAAGCAGC TGAATCCTGT CTTTGGAAAG
    TCATTTGATA TAGAGGCCAC GTTCCCAATG GAGTCTATGC TGACTGTGGC AGTGTATGAC
    TGGGATTTAG TCGGAACTGA TGACCTGATT GGTGAGACTA AGATTGATTT GGAGAACAGA
    TACTACAGCA AACACAGAGC CACATGTGGC ATTGCTTCCA ACTACTCTGT CCATGGTTAT
    AATGTATGGA GAGATCCTCA GAAACCAGCT CAGATCCTGG CTAAGTTGTG CAAAGAAGGT
    AAACTGGATG GACCTCACTA TGGACCTGGG GGAAGAGTCA AAGTTGCAAA CCGGATCTTT
    CTTGGGCCGA CAGAAATTGA GGATGAGAGT GGTCTGAAAA AACAAACAGA AGAACACTTG
    GCGCTCACAG TCCTTAGACA TTGGGAGGAA ATCCCACGTG TTGGTTGCAA ACTCATCCCT
    GAGCATGTGG AGACGAGGCC TCTCCTCAAC CCAGACAAGC CAGGCATAGA GCAGGGAAGG
    ATTGAAATGT GGGTGGACAT GTTTCCTATG GATGTCCCAG CACCAGGACC AGCCATTGAT
    ATATCGCCAC GCAAACCAAA GAGATATGAG CTCAGGGTGA TAATATGGAA TACTGACGAG
    GTAATTCTGG AAGACGATGA TTACTTCACA GGGGAAAAGT CCAGTGACAT TTTTGTGAGG
    GGCTGGCTTA AAGGACAGCA GGAGGACAAA CAGGACACCG ATGTACATTA TCACTCTCTA
    ACTGGAGAGG GAAACTTCAA CTGGCGCTTT GTCTTCCCCT TTGACTATCT CATGGCCGAG
    GAGAAGATTG TTATCTCCAA GAAGGAATCA ATGTTTTCCT GGGATGAGAC TGAGTATAAG
    ATCCCTGCTC GACTCACCCT TCAAGTATGG GATGCCGATC ACTTTTCTGC AGATGATTTT
    CTGGGTGCGA TTGAGTTGGA CTTGAATAAG TTTCCGCGTG GGGCAAAAAC AGCCAAGCAG
    TGCTCTCTAG ACATGGTTCT CAAAGAGCAT GAGCTGCCAA CCATCTCCAT CTTCAAACAA
    AAGAGAGTCA AGGGCTGGTG GCCATTTGTG GCCCAAAATG AGAATGATGA ATTTGAGCTA
    ACGGGAAAAG TAGAGGCAGA GCTCCATCTG TTGACAGCAG AGGAGGCAGA GAAGAGTCCA
    GTCGGCCTGG GGAGGAATGA TCCGGAGCCA CTTGAGAAAC CAAATCGTCC AACTACCAGC
    CTGTCGTGGC TGTTGAGTCC GATTCGGGTG ACCAGCATAA TGTGTTGGCG GCAGTATAAG
    TTGGTCACAG TGGTGACTCT GCTCATCATA ACCGGGCTGA TCTTTTTGGG CCTCCTGTTC
    TTTTCTCTGC CTTCAGCTTT CATGCAGAAG CTCTTCAACA TCTCTGGATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021324116.1
    CDS300..6410
    Translation

    Target ORF information:

    RefSeq Version XM_021468441.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio otoferlin a (otofa), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021468441.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGGCTTTAG TGGTGCATCT GAAGACTGTC ACGGAGCTCC GTGGGAAGGG GGACCGAATC 
    GCTAAAGTCA CTTTCAGAGG TCTCTCCTTC TTCTCTCGGG TGTTGGAAAA CTGCGAGGAT
    GAAGCTCGAT TTGAGCAGGC GTTCCGATGG CCAATTGGGA GTCAAGTGGA TGGCGACGAG
    ATGCTGGAGA TCCAAGTGTT TAATTACAGC AAAGTCTTCA CTAATAGGTT AATCGGCACA
    TTCCGCATGG TTCTTCAGAA GGTGGTGGAG GAGGGACACC TGGAGGTGTC TGACACACTC
    ATTGACGACA ATAACACAGC CATACAGACA ACCATTTCCA TAGAGATCAA ATATCAGACC
    ATGGACGGGA GTGTAAAGGT GTGGAGCGAT GGTGAATTCC TTGATATTCC AGATGACCTT
    GATGGAACCT TCCAGTTTGA GACAGATAGT CTTCTCTCAG GACGCAGCCA GAGTTCTGGA
    ACTTCACCTG GACGATCCAT TCATGGCATT CCCACATTCC GCAAGGCAGG AAAAGGAGTG
    TTCTCAGCCA TGAAGCTGGG CAAGACCCGC ACCTCTAAAG ATGACCACAA AAAAGGAGAT
    GATGCTGCCA TTCTGGATGC AGAAGATCTG GATCGAAAAG CCATGCGTCT AGGCGGCATT
    CTTGACCCTG ATACAATCTC ACTGGCCTCG GTTACTGCAG TGACCACTAA TGTCTCCAAC
    AAAAGATCAA AGCCTGACAT TAAAATGGAG CCGAGTTCAG GGCGTCCGGT TGATTATCAG
    ATCAGTGTTA CAGTAATCGA GGCCCGTCAG CTGGTCGGAT TGAACATGGA CCCTGTGGTT
    TGCGTGGAAA TTGGAGAGGA GAAGAAGTAC ACGTCAATGA AGGAGTCAAC AAACTGCCCT
    TACTACAATG AGTATTTTGT CTTCGACTTC CACGTGCCTC CAGACGTCAT GTTTGACAAA
    ATTATAAAGA TTTCAGTAAT ACATTCTAAA AACCTTCTGC GAAGCGGCAC CCTCGTGGGC
    ACCTTCAAAC TAGACGTGGG GACTGTTTAC ACACAACCTG AACATCAGTT TCATCACAAA
    TGGGCGATGC TGTCAGACCC TGATGACATC ACGACTGGCT GCAAAGGTTA TGTTAAATGT
    GACATCGCAG TCGTGGGAAA AGGAGACAAC ATCAAGACTC CACACAAGGC CAGTGAAGCA
    GACGAGGATG ACATAGAGGG GAATCTTCTC TTGCCGGAGG GTGTTCCATC AGAGAGACAG
    TGGGCTCGGT TTTATGTTAA GATTTATAGA GCTGAGGGTC TGCCTAAAAT GAACACCAGT
    ATCATGGCCA ACGTGAAGAA AGCCTTTATT GGGGAAAACA GGGACCTTGT GGACCCTTAC
    GTCCTGGTGC AATTTGCAGG GCAAAAGGGC AAAACATCTG TTCAGAAGAG CAGCTACGAG
    CCCATCTGGA ACGAGCAAGT CATCTTCACG GAGATGTTCC CACCTTTGTG TAGGCGACTG
    AAAGTTCAGA TCCGTGATTC AGACAAGGTG AACGATGTTG CCATAGGAAC CCACTTCATT
    GATCTACGAA AGGTTTCCAA TGATGGAGAC AAAGGTTTCT TGCCCACTAT GGGTCCGGCT
    TGGGTGAATA TGTATGGGTC GACCCGTAAC TACACCCTGA TGGACGAACA CCAGGACCTG
    AATGAGGGGT TGGGGGAAGG GGTGTCCTTC AGAGCACGTC TGCTCATTAG TATCGCGGTG
    GAGATTCTAG ACACCACCTC TGCTGAGATC ATGAGCTCCA CTGAAGTACA CATGGAGCCG
    GTGTCCAACA TCTCAGAGAG TGCTACAGGA AAGATGGAAG AATTTTTCCT TTTCGGGTCA
    TTCTTGGAGG CCACAATGAT AGACAGAAAA ATAGGAGATA AACCCATCAG CTTTGAAGTC
    ACCATCGGTA ACTATGGTAA CCAGATTGAT GGTGTGAGCA AGCCTGCATT AGCGAAGAAG
    AAGAAGGAGG GTGGAGGAGA GAGTGAAGAA GAGGAGTCTG AGCTTATCCA CAACTCCAGC
    GAGGAAGAGG CAGAGGATGA TGGAGATCTG ACCTCTGTAC CGTCTACACC TCCTATGAAG
    CCTGTTATCA CCGACAGAAA TTACTTCCAC TTGCCTTACT TTGAAAAGAA ACCCTGCATT
    TACATCAAGA GCTGGTGGCA GGACCAGCGG AGACGTCTCT ACAACTCCAA CATAATGGAC
    AAGATAGCTG ATAAACTGGA GGAAGGTCTG AATGATGTTC AAGAGATCAT AAAAACAGAG
    AAGGCATATC CAGAACGAAG ACTAAGAGGT GTATTAGAAG AACTTAGCAC AAGCTGCAGC
    CGGTTTGTTA CACTTGCAAA CAAGGATCAG AATCTTTCTG GAAGAACCAA GCTGGACAGA
    GAGAGGCTGA AGTCCTGTAT GAGAGAAATG GAGAGTATGG GTCAGCAGGC GAAGACCATC
    CGCACACAAG TGAAAAGAAA CACAGTTCGA GATAAACTCA AGCTAGTGTT GAATTTCCTT
    CAGAGGCTTC GATTCTTAGC AGACGAGCCC CAGCACAGCA TCCCTGATGT GTTCATATGG
    ATGATTAGCA ACAACAAACG CATCGCATAC GCCCGCATAC CCTCCAAAGA CATCCTCTAC
    TCAATTGTCG ATGAAGAGAT GGGCAAAGAC TGTGGAAAAG TTAAAGCTGT TTTTCTCAGG
    CTGCCTGGAA AGAAAGGTTT TGGGCCTGCT GGCTGGACAG TTCAGGCTAA ATTAGAGATG
    TATTTGTGGC TGGGCCTGAA TAAACAGAGG AAAGACTTCC TGATTGGCCT CCCTAGTGGC
    TTTGAAGAAA ACAAGGCAGT AAAGGGAATT GGCATACAAG CTGTCCCTCC CATCAGCCTA
    GTTTATAACA TGAAGCAGGT GTTTCAGCTG AGGGCCCACA TGTATCAAGC TCGCAGTCTG
    TTTGCTGCGG ACACTAGTGG CCTGTCTGAT CCATTTGCAA GGGTTTTCTT CTCCACCCAC
    AGCCAGGTCA CGGAGGTCCT CAGTGAGACT CTCTGTCCAA CATGGGATCA GCTGCTTGTG
    TTTGATAATG TTGAACTTTA CGGTGAGGCC GGTGAACTCC GTGATGACCC TCCAATCATT
    GTCATTGAGT TGTATGATCA AGACACTGTG GGGAAAGCTG AGTTTATTGG CCGAACATTT
    GCAAAACCAC TTACTAAGAT GGTTGATGAA CACTACGGCC CACCGCGGTT TCCTCCCCAG
    CTCGAGTATT ATCAGATCTA CCGAGGAAAC TGTGCTGCGG GAGATCTATT GGCTGCTTTT
    GAGCTTCTAC AGATTCCTTA TGACGATGAG GAGATCAGAA GAGCTCTTAT TGCTGTCCAT
    GACTTTGCTG TGCCTCAGAT CAAGATTGGT CCAGCAGGGC GGGCAGCACT TCCTCCAATC
    GATGGACCGA CCGATTCTGA CCGTGGGCCC ATTCTGCCTG TTCCATTGGG AATTCGACCA
    GTTCTCAGCA GATATCGCAT CGAGGTCCTG TTTTGGGGTC TGAGGGACCT TAAAAGGGTC
    AACCTGGCTC AGGTGGACCG ACCTCGTGTG GACATTGAGT GTGCAGGGAA AGGAGTTCAG
    TCAGCCCTAA TTCAAAACTA CAAGAAGAAT CCAAACTTCA GCACATTAGT AAAGTGGTTT
    GAAGTGGATC TGCCAGAAAA TGAGCTGCTC CATCCACCAC TTAATATTCG GGTAGTGGAT
    TGCAGGGCAT TCGGACGCTA CATTTTGGTG GGGTCTCATG CTGTGACCAC CCTTCGCAAG
    TTCATCTACA GCCCCCCAGA CAAGACTGCT AACAACTGGG CTCACACAGC TAGACTGGCC
    AATGGCTACC TGGCTCTCAG CAATGGGACG TCTCATTCCC GCCCCCAATC CCGTCCTGTT
    TCTTATCCCT CAGGTGACAT TGTGGTCAAC ATGAGCCCTG AACCTAACAT TAAGAAGATG
    GACACGGTTG TCAAGATTGA AGCTAATGAG GATGAGAAGG AGAAAGAAAA GAAGAAGAAG
    AAGAAGAAGA AAGGAGAAGA AGTGGAGGAA GAGGAGCCGG ATGAGAGCAT GCTGGACTGG
    TGGTCCAAAT ACTTTGCCTC AATAGAGACT ATGATGGAGA ATCTAAGAGC TCAGGAGGCC
    GCTCAGGCAG AGGCAGAGGA AAGAGAAGAT TTGGAGATTG CAGCAGAGAG TGCAGAGATC
    AAAGCTGATG ACTTTCCTAT GAAAGGCACC AAACCTAAAG AGAAGAGCAA AGACAAGAAG
    AGCACCAAGG ACAAAAAGAA GAACAACGAT GGCACAGAAA AACGACCTCC AAAACCAAAA
    GTTGATGAAC TCATGGTGTA CAATAAAGAG CTGGAGAGTG AGTTCGGTAG CTTTGAGGAC
    TGGCTTCACA CCTTCAACCT GTACCGAGGA AAGGCTGGAG ATGATGATGA CCACAATGTG
    GTTGATGAGG ACAGAATTGT GGGCAGATTC AAGGGCTCCC TCTGTATGTA TAAACTACCA
    CTGTCTGAGG AGATCACCAG AGAGGCAGGA TTTGATCCAA ACATGGGCAT GTTCCAGAGT
    ATTCCTCACA ATGACCCTAT CAACGTTCTT GTCAGGATTT ATATCATTCG AGCCACAGAC
    CTGCATCCTG CAGATATAAA TGGAAAGGCC GACCCTTATA TAGTCATTAA ATTGGGAAAG
    TCAGACATTC GAGACAAGGA GAACTACATA TCCAAGCAGC TGAATCCTGT CTTTGGAAAG
    TCATTTGATA TAGAGGCCAC GTTCCCAATG GAGTCTATGC TGACTGTGGC AGTGTATGAC
    TGGGATTTAG TCGGAACTGA TGACCTGATT GGTGAGACTA AGATTGATTT GGAGAACAGA
    TACTACAGCA AACACAGAGC CACATGTGGC ATTGCTTCCA ACTACTCTGT CCATGGTTAT
    AATGTATGGA GAGATCCTCA GAAACCAGCT CAGATCCTGG CTAAGTTGTG CAAAGAAGGT
    AAACTGGATG GACCTCACTA TGGACCTGGG GGAAGAGTCA AAGTTGCAAA CCGGATCTTT
    CTTGGGCCGA CAGAAATTGA GGATGAGAGT GGTCTGAAAA AACAAACAGA AGAACACTTG
    GCGCTCACAG TCCTTAGACA TTGGGAGGAA ATCCCACGTG TTGGTTGCAA ACTCATCCCT
    GAGCATGTGG AGACGAGGCC TCTCCTCAAC CCAGACAAGC CAGGCATAGA GCAGGGAAGG
    ATTGAAATGT GGGTGGACAT GTTTCCTATG GATGTCCCAG CACCAGGACC AGCCATTGAT
    ATATCGCCAC GCAAACCAAA GAGATATGAG CTCAGGGTGA TAATATGGAA TACTGACGAG
    GTAATTCTGG AAGACGATGA TTACTTCACA GGGGAAAAGT CCAGTGACAT TTTTGTGAGG
    GGCTGGCTTA AAGGACAGCA GGAGGACAAA CAGGACACCG ATGTACATTA TCACTCTCTA
    ACTGGAGAGG GAAACTTCAA CTGGCGCTTT GTCTTCCCCT TTGACTATCT CATGGCCGAG
    GAGAAGATTG TTATCTCCAA GAAGGAATCA ATGTTTTCCT GGGATGAGAC TGAGTATAAG
    ATCCCTGCTC GACTCACCCT TCAAGTATGG GATGCCGATC ACTTTTCTGC AGATGATTTT
    CTGGGTGCGA TTGAGTTGGA CTTGAATAAG TTTCCGCGTG GGGCAAAAAC AGCCAAGCAG
    TGCTCTCTAG ACATGGTTCT CAAAGAGCAT GAGCTGCCAA CCATCTCCAT CTTCAAACAA
    AAGAGAGTCA AGGGCTGGTG GCCATTTGTG GCCCAAAATG AGAATGATGA ATTTGAGCTA
    ACGGGAAAAG TAGAGGCAGA GCTCCATCTG TTGACAGCAG AGGAGGCAGA GAAGAGTCCA
    GTCGGCCTGG GGAGGAATGA TCCGGAGCCA CTTGAGAAAC CAAATCGTCC AACTACCAGC
    CTGTCGTGGC TGTTGAGTCC GATTCGGGTG ACCAGCATAA TGTGTTGGCG GCAGTATAAG
    TTGGTCACAG TGGTGACTCT GCTCATCATA ACCGGGCTGA TCTTTTTGGG CCTCCTGTTC
    TTTTCTCTGC CTTCAGCTTT CATGCAGAAG CTCTTCAACA TCTCTGGATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42609
    Clone ID Related Accession (Same CDS sequence) XM_009294738.2 , XM_009294738.3
    Accession Version XM_009294738.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6111bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product otoferlin isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009294738.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGGCTTTAG TGGTGCATCT GAAGACTGTC ACGGAGCTCC GTGGGAAGGG GGACCGAATC 
    GCTAAAGTCA CTTTCAGAGG TCTCTCCTTC TTCTCTCGGG TGTTGGAAAA CTGCGAGGAT
    GAAGCTCGAT TTGAGCAGGC GTTCCGATGG CCAATTGGGA GTCAAGTGGA TGGCGACGAG
    ATGCTGGAGA TCCAAGTGTT TAATTACAGC AAAGTCTTCA CTAATAGGTT AATCGGCACA
    TTCCGCATGG TTCTTCAGAA GGTGGTGGAG GAGGGACACC TGGAGGTGTC TGACACACTC
    ATTGACGACA ATAACACAGC CATACAGACA ACCATTTCCA TAGAGATCAA ATATCAGACC
    ATGGACGGGA GTGTAAAGGT GTGGAGCGAT GGTGAATTCC TTGATATTCC AGATGACCTT
    GATGGAACCT TCCAGTTTGA GACAGATAGT CTTCTCTCAG GACGCAGCCA GAGTTCTGGA
    ACTTCACCTG GACGATCCAT TCATGGCATT CCCACATTCC GCAAGGCAGG AAAAGGAGTG
    TTCTCAGCCA TGAAGCTGGG CAAGACCCGC ACCTCTAAAG ATGACCACAA AAAAGGAGAT
    GATGCTGCCA TTCTGGATGC AGAAGATCTG GATCGAAAAG CCATGCGTCT AGGCGGCATT
    CTTGACCCTG ATACAATCTC ACTGGCCTCG GTTACTGCAG TGACCACTAA TGTCTCCAAC
    AAAAGATCAA AGCCTGACAT TAAAATGGAG CCGAGTTCAG GGCGTCCGGT TGATTATCAG
    ATCAGTGTTA CAGTAATCGA GGCCCGTCAG CTGGTCGGAT TGAACATGGA CCCTGTGGTT
    TGCGTGGAAA TTGGAGAGGA GAAGAAGTAC ACGTCAATGA AGGAGTCAAC AAACTGCCCT
    TACTACAATG AGTATTTTGT CTTCGACTTC CACGTGCCTC CAGACGTCAT GTTTGACAAA
    ATTATAAAGA TTTCAGTAAT ACATTCTAAA AACCTTCTGC GAAGCGGCAC CCTCGTGGGC
    ACCTTCAAAC TAGACGTGGG GACTGTTTAC ACACAACCTG AACATCAGTT TCATCACAAA
    TGGGCGATGC TGTCAGACCC TGATGACATC ACGACTGGCT GCAAAGGTTA TGTTAAATGT
    GACATCGCAG TCGTGGGAAA AGGAGACAAC ATCAAGACTC CACACAAGGC CAGTGAAGCA
    GACGAGGATG ACATAGAGGG GAATCTTCTC TTGCCGGAGG GTGTTCCATC AGAGAGACAG
    TGGGCTCGGT TTTATGTTAA GATTTATAGA GCTGAGGGTC TGCCTAAAAT GAACACCAGT
    ATCATGGCCA ACGTGAAGAA AGCCTTTATT GGGGAAAACA GGGACCTTGT GGACCCTTAC
    GTCCTGGTGC AATTTGCAGG GCAAAAGGGC AAAACATCTG TTCAGAAGAG CAGCTACGAG
    CCCATCTGGA ACGAGCAAGT CATCTTCACG GAGATGTTCC CACCTTTGTG TAGGCGACTG
    AAAGTTCAGA TCCGTGATTC AGACAAGGTG AACGATGTTG CCATAGGAAC CCACTTCATT
    GATCTACGAA AGGTTTCCAA TGATGGAGAC AAAGGTTTCT TGCCCACTAT GGGTCCGGCT
    TGGGTGAATA TGTATGGGTC GACCCGTAAC TACACCCTGA TGGACGAACA CCAGGACCTG
    AATGAGGGGT TGGGGGAAGG GGTGTCCTTC AGAGCACGTC TGCTCATTAG TATCGCGGTG
    GAGATTCTAG ACACCACCTC TGCTGAGATC ATGAGCTCCA CTGAAGTACA CATGGAGCCG
    GTGTCCAACA TCTCAGAGAG TGCTACAGGA AAGATGGAAG AATTTTTCCT TTTCGGGTCA
    TTCTTGGAGG CCACAATGAT AGACAGAAAA ATAGGAGATA AACCCATCAG CTTTGAAGTC
    ACCATCGGTA ACTATGGTAA CCAGATTGAT GGTGTGAGCA AGCCTGCATT AGCGAAGAAG
    AAGAAGGAGG GTGGAGGAGA GAGTGAAGAA GAGGAGTCTG AGCTTATCCA CAACTCCAGC
    GAGGAAGAGG CAGAGGATGA TGGAGATCTG ACCTCTGTAC CGTCTACACC TCCTATGAAG
    CCTGTTATCA CCGACAGAAA TTACTTCCAC TTGCCTTACT TTGAAAAGAA ACCCTGCATT
    TACATCAAGA GCTGGTGGCA GGACCAGCGG AGACGTCTCT ACAACTCCAA CATAATGGAC
    AAGATAGCTG ATAAACTGGA GGAAGGTCTG AATGATGTTC AAGAGATCAT AAAAACAGAG
    AAGGCATATC CAGAACGAAG ACTAAGAGGT GTATTAGAAG AACTTAGCAC AAGCTGCAGC
    CGGTTTGTTA CACTTGCAAA CAAGGATCAG AATCTTTCTG GAAGAACCAA GCTGGACAGA
    GAGAGGCTGA AGTCCTGTAT GAGAGAAATG GAGAGTATGG GTCAGCAGGC GAAGACCATC
    CGCACACAAG TGAAAAGAAA CACAGTTCGA GATAAACTCA AGCTAGTGTT GAATTTCCTT
    CAGAGGCTTC GATTCTTAGC AGACGAGCCC CAGCACAGCA TCCCTGATGT GTTCATATGG
    ATGATTAGCA ACAACAAACG CATCGCATAC GCCCGCATAC CCTCCAAAGA CATCCTCTAC
    TCAATTGTCG ATGAAGAGAT GGGCAAAGAC TGTGGAAAAG TTAAAGCTGT TTTTCTCAGG
    CTGCCTGGAA AGAAAGGTTT TGGGCCTGCT GGCTGGACAG TTCAGGCTAA ATTAGAGATG
    TATTTGTGGC TGGGCCTGAA TAAACAGAGG AAAGACTTCC TGATTGGCCT CCCTAGTGGC
    TTTGAAGAAA ACAAGGCAGT AAAGGGAATT GGCATACAAG CTGTCCCTCC CATCAGCCTA
    GTTTATAACA TGAAGCAGGT GTTTCAGCTG AGGGCCCACA TGTATCAAGC TCGCAGTCTG
    TTTGCTGCGG ACACTAGTGG CCTGTCTGAT CCATTTGCAA GGGTTTTCTT CTCCACCCAC
    AGCCAGGTCA CGGAGGTCCT CAGTGAGACT CTCTGTCCAA CATGGGATCA GCTGCTTGTG
    TTTGATAATG TTGAACTTTA CGGTGAGGCC GGTGAACTCC GTGATGACCC TCCAATCATT
    GTCATTGAGT TGTATGATCA AGACACTGTG GGGAAAGCTG AGTTTATTGG CCGAACATTT
    GCAAAACCAC TTACTAAGAT GGTTGATGAA CACTACGGCC CACCGCGGTT TCCTCCCCAG
    CTCGAGTATT ATCAGATCTA CCGAGGAAAC TGTGCTGCGG GAGATCTATT GGCTGCTTTT
    GAGCTTCTAC AGATTCCTTA TGACGATGAG GAGATCAGAA GAGCTCTTAT TGCTGTCCAT
    GACTTTGCTG TGCCTCAGAT CAAGATTGGT CCAGCAGGGC GGGCAGCACT TCCTCCAATC
    GATGGACCGA CCGATTCTGA CCGTGGGCCC ATTCTGCCTG TTCCATTGGG AATTCGACCA
    GTTCTCAGCA GATATCGCAT CGAGGTCCTG TTTTGGGGTC TGAGGGACCT TAAAAGGGTC
    AACCTGGCTC AGGTGGACCG ACCTCGTGTG GACATTGAGT GTGCAGGGAA AGGAGTTCAG
    TCAGCCCTAA TTCAAAACTA CAAGAAGAAT CCAAACTTCA GCACATTAGT AAAGTGGTTT
    GAAGTGGATC TGCCAGAAAA TGAGCTGCTC CATCCACCAC TTAATATTCG GGTAGTGGAT
    TGCAGGGCAT TCGGACGCTA CATTTTGGTG GGGTCTCATG CTGTGACCAC CCTTCGCAAG
    TTCATCTACA GCCCCCCAGA CAAGACTGCT AACAACTGGG CTCACACAGC TAGACTGGCC
    AATGGCTACC TGGCTCTCAG CAATGGGACG TCTCATTCCC GCCCCCAATC CCGTCCTGTT
    TCTTATCCCT CAGGTGACAT TGTGGTCAAC ATGAGCCCTG AACCTAACAT TAAGAAGATG
    GACACGGTTG TCAAGATTGA AGCTACCACC GATGCGGTTG TTAAAGTTGA CTTGAATGAG
    GATGAGAAGG AGAAAGAAAA GAAGAAGAAG AAGAAGAAGA AAGGAGAAGA AGTGGAGGAA
    GAGGAGCCGG ATGAGAGCAT GCTGGACTGG TGGTCCAAAT ACTTTGCCTC AATAGAGACT
    ATGATGGAGA ATCTAAGAGC TCAGGAGGCC GCTCAGGCAG AGGCAGAGGA AAGAGAAGAT
    TTGGAGATTG CAGCAGAGAG TGCAGGCACC AAACCTAAAG AGAAGAGCAA AGACAAGAAG
    AGCACCAAGG ACAAAAAGAA GAACAACGAT GGCACAGAAA AACGACCTCC AAAACCAAAA
    GTTGATGAAC TCATGGTGTA CAATAAAGAG CTGGAGAGTG AGTTCGGTAG CTTTGAGGAC
    TGGCTTCACA CCTTCAACCT GTACCGAGGA AAGGCTGGAG ATGATGATGA CCACAATGTG
    GTTGATGAGG ACAGAATTGT GGGCAGATTC AAGGGCTCCC TCTGTATGTA TAAACTACCA
    CTGTCTGAGG AGATCACCAG AGAGGCAGGA TTTGATCCAA ACATGGGCAT GTTCCAGAGT
    ATTCCTCACA ATGACCCTAT CAACGTTCTT GTCAGGATTT ATATCATTCG AGCCACAGAC
    CTGCATCCTG CAGATATAAA TGGAAAGGCC GACCCTTATA TAGTCATTAA ATTGGGAAAG
    TCAGACATTC GAGACAAGGA GAACTACATA TCCAAGCAGC TGAATCCTGT CTTTGGAAAG
    TCATTTGATA TAGAGGCCAC GTTCCCAATG GAGTCTATGC TGACTGTGGC AGTGTATGAC
    TGGGATTTAG TCGGAACTGA TGACCTGATT GGTGAGACTA AGATTGATTT GGAGAACAGA
    TACTACAGCA AACACAGAGC CACATGTGGC ATTGCTTCCA ACTACTCTGT CCATGGTTAT
    AATGTATGGA GAGATCCTCA GAAACCAGCT CAGATCCTGG CTAAGTTGTG CAAAGAAGGT
    AAACTGGATG GACCTCACTA TGGACCTGGG GGAAGAGTCA AAGTTGCAAA CCGGATCTTT
    CTTGGGCCGA CAGAAATTGA GGATGAGAGT GGTCTGAAAA AACAAACAGA AGAACACTTG
    GCGCTCACAG TCCTTAGACA TTGGGAGGAA ATCCCACGTG TTGGTTGCAA ACTCATCCCT
    GAGCATGTGG AGACGAGGCC TCTCCTCAAC CCAGACAAGC CAGGCATAGA GCAGGGAAGG
    ATTGAAATGT GGGTGGACAT GTTTCCTATG GATGTCCCAG CACCAGGACC AGCCATTGAT
    ATATCGCCAC GCAAACCAAA GAGATATGAG CTCAGGGTGA TAATATGGAA TACTGACGAG
    GTAATTCTGG AAGACGATGA TTACTTCACA GGGGAAAAGT CCAGTGACAT TTTTGTGAGG
    GGCTGGCTTA AAGGACAGCA GGAGGACAAA CAGGACACCG ATGTACATTA TCACTCTCTA
    ACTGGAGAGG GAAACTTCAA CTGGCGCTTT GTCTTCCCCT TTGACTATCT CATGGCCGAG
    GAGAAGATTG TTATCTCCAA GAAGGAATCA ATGTTTTCCT GGGATGAGAC TGAGTATAAG
    ATCCCTGCTC GACTCACCCT TCAAGTATGG GATGCCGATC ACTTTTCTGC AGATGATTTT
    CTGGGTGCGA TTGAGTTGGA CTTGAATAAG TTTCCGCGTG GGGCAAAAAC AGCCAAGCAG
    TGCTCTCTAG ACATGGTTCT CAAAGAGCAT GAGCTGCCAA CCATCTCCAT CTTCAAACAA
    AAGAGAGTCA AGGGCTGGTG GCCATTTGTG GCCCAAAATG AGAATGATGA ATTTGAGCTA
    ACGGGAAAAG TAGAGGCAGA GCTCCATCTG TTGACAGCAG AGGAGGCAGA GAAGAGTCCA
    GTCGGCCTGG GGAGGAATGA TCCGGAGCCA CTTGAGAAAC CAAATCGTCC AACTACCAGC
    CTGTCGTGGC TGTTGAGTCC GATTCGGGTG ACCAGCATAA TGTGTTGGCG GCAGTATAAG
    TTGGTCACAG TGGTGACTCT GCTCATCATA ACCGGGCTGA TCTTTTTGGG CCTCCTGTTC
    TTTTCTCTGC CTTCAGCTTT CATGCAGAAG CTCTTCAACA TCTCTGGATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009293013.1
    CDS303..6413
    Translation

    Target ORF information:

    RefSeq Version XM_009294738.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio otoferlin a (otofa), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009294738.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGGCTTTAG TGGTGCATCT GAAGACTGTC ACGGAGCTCC GTGGGAAGGG GGACCGAATC 
    GCTAAAGTCA CTTTCAGAGG TCTCTCCTTC TTCTCTCGGG TGTTGGAAAA CTGCGAGGAT
    GAAGCTCGAT TTGAGCAGGC GTTCCGATGG CCAATTGGGA GTCAAGTGGA TGGCGACGAG
    ATGCTGGAGA TCCAAGTGTT TAATTACAGC AAAGTCTTCA CTAATAGGTT AATCGGCACA
    TTCCGCATGG TTCTTCAGAA GGTGGTGGAG GAGGGACACC TGGAGGTGTC TGACACACTC
    ATTGACGACA ATAACACAGC CATACAGACA ACCATTTCCA TAGAGATCAA ATATCAGACC
    ATGGACGGGA GTGTAAAGGT GTGGAGCGAT GGTGAATTCC TTGATATTCC AGATGACCTT
    GATGGAACCT TCCAGTTTGA GACAGATAGT CTTCTCTCAG GACGCAGCCA GAGTTCTGGA
    ACTTCACCTG GACGATCCAT TCATGGCATT CCCACATTCC GCAAGGCAGG AAAAGGAGTG
    TTCTCAGCCA TGAAGCTGGG CAAGACCCGC ACCTCTAAAG ATGACCACAA AAAAGGAGAT
    GATGCTGCCA TTCTGGATGC AGAAGATCTG GATCGAAAAG CCATGCGTCT AGGCGGCATT
    CTTGACCCTG ATACAATCTC ACTGGCCTCG GTTACTGCAG TGACCACTAA TGTCTCCAAC
    AAAAGATCAA AGCCTGACAT TAAAATGGAG CCGAGTTCAG GGCGTCCGGT TGATTATCAG
    ATCAGTGTTA CAGTAATCGA GGCCCGTCAG CTGGTCGGAT TGAACATGGA CCCTGTGGTT
    TGCGTGGAAA TTGGAGAGGA GAAGAAGTAC ACGTCAATGA AGGAGTCAAC AAACTGCCCT
    TACTACAATG AGTATTTTGT CTTCGACTTC CACGTGCCTC CAGACGTCAT GTTTGACAAA
    ATTATAAAGA TTTCAGTAAT ACATTCTAAA AACCTTCTGC GAAGCGGCAC CCTCGTGGGC
    ACCTTCAAAC TAGACGTGGG GACTGTTTAC ACACAACCTG AACATCAGTT TCATCACAAA
    TGGGCGATGC TGTCAGACCC TGATGACATC ACGACTGGCT GCAAAGGTTA TGTTAAATGT
    GACATCGCAG TCGTGGGAAA AGGAGACAAC ATCAAGACTC CACACAAGGC CAGTGAAGCA
    GACGAGGATG ACATAGAGGG GAATCTTCTC TTGCCGGAGG GTGTTCCATC AGAGAGACAG
    TGGGCTCGGT TTTATGTTAA GATTTATAGA GCTGAGGGTC TGCCTAAAAT GAACACCAGT
    ATCATGGCCA ACGTGAAGAA AGCCTTTATT GGGGAAAACA GGGACCTTGT GGACCCTTAC
    GTCCTGGTGC AATTTGCAGG GCAAAAGGGC AAAACATCTG TTCAGAAGAG CAGCTACGAG
    CCCATCTGGA ACGAGCAAGT CATCTTCACG GAGATGTTCC CACCTTTGTG TAGGCGACTG
    AAAGTTCAGA TCCGTGATTC AGACAAGGTG AACGATGTTG CCATAGGAAC CCACTTCATT
    GATCTACGAA AGGTTTCCAA TGATGGAGAC AAAGGTTTCT TGCCCACTAT GGGTCCGGCT
    TGGGTGAATA TGTATGGGTC GACCCGTAAC TACACCCTGA TGGACGAACA CCAGGACCTG
    AATGAGGGGT TGGGGGAAGG GGTGTCCTTC AGAGCACGTC TGCTCATTAG TATCGCGGTG
    GAGATTCTAG ACACCACCTC TGCTGAGATC ATGAGCTCCA CTGAAGTACA CATGGAGCCG
    GTGTCCAACA TCTCAGAGAG TGCTACAGGA AAGATGGAAG AATTTTTCCT TTTCGGGTCA
    TTCTTGGAGG CCACAATGAT AGACAGAAAA ATAGGAGATA AACCCATCAG CTTTGAAGTC
    ACCATCGGTA ACTATGGTAA CCAGATTGAT GGTGTGAGCA AGCCTGCATT AGCGAAGAAG
    AAGAAGGAGG GTGGAGGAGA GAGTGAAGAA GAGGAGTCTG AGCTTATCCA CAACTCCAGC
    GAGGAAGAGG CAGAGGATGA TGGAGATCTG ACCTCTGTAC CGTCTACACC TCCTATGAAG
    CCTGTTATCA CCGACAGAAA TTACTTCCAC TTGCCTTACT TTGAAAAGAA ACCCTGCATT
    TACATCAAGA GCTGGTGGCA GGACCAGCGG AGACGTCTCT ACAACTCCAA CATAATGGAC
    AAGATAGCTG ATAAACTGGA GGAAGGTCTG AATGATGTTC AAGAGATCAT AAAAACAGAG
    AAGGCATATC CAGAACGAAG ACTAAGAGGT GTATTAGAAG AACTTAGCAC AAGCTGCAGC
    CGGTTTGTTA CACTTGCAAA CAAGGATCAG AATCTTTCTG GAAGAACCAA GCTGGACAGA
    GAGAGGCTGA AGTCCTGTAT GAGAGAAATG GAGAGTATGG GTCAGCAGGC GAAGACCATC
    CGCACACAAG TGAAAAGAAA CACAGTTCGA GATAAACTCA AGCTAGTGTT GAATTTCCTT
    CAGAGGCTTC GATTCTTAGC AGACGAGCCC CAGCACAGCA TCCCTGATGT GTTCATATGG
    ATGATTAGCA ACAACAAACG CATCGCATAC GCCCGCATAC CCTCCAAAGA CATCCTCTAC
    TCAATTGTCG ATGAAGAGAT GGGCAAAGAC TGTGGAAAAG TTAAAGCTGT TTTTCTCAGG
    CTGCCTGGAA AGAAAGGTTT TGGGCCTGCT GGCTGGACAG TTCAGGCTAA ATTAGAGATG
    TATTTGTGGC TGGGCCTGAA TAAACAGAGG AAAGACTTCC TGATTGGCCT CCCTAGTGGC
    TTTGAAGAAA ACAAGGCAGT AAAGGGAATT GGCATACAAG CTGTCCCTCC CATCAGCCTA
    GTTTATAACA TGAAGCAGGT GTTTCAGCTG AGGGCCCACA TGTATCAAGC TCGCAGTCTG
    TTTGCTGCGG ACACTAGTGG CCTGTCTGAT CCATTTGCAA GGGTTTTCTT CTCCACCCAC
    AGCCAGGTCA CGGAGGTCCT CAGTGAGACT CTCTGTCCAA CATGGGATCA GCTGCTTGTG
    TTTGATAATG TTGAACTTTA CGGTGAGGCC GGTGAACTCC GTGATGACCC TCCAATCATT
    GTCATTGAGT TGTATGATCA AGACACTGTG GGGAAAGCTG AGTTTATTGG CCGAACATTT
    GCAAAACCAC TTACTAAGAT GGTTGATGAA CACTACGGCC CACCGCGGTT TCCTCCCCAG
    CTCGAGTATT ATCAGATCTA CCGAGGAAAC TGTGCTGCGG GAGATCTATT GGCTGCTTTT
    GAGCTTCTAC AGATTCCTTA TGACGATGAG GAGATCAGAA GAGCTCTTAT TGCTGTCCAT
    GACTTTGCTG TGCCTCAGAT CAAGATTGGT CCAGCAGGGC GGGCAGCACT TCCTCCAATC
    GATGGACCGA CCGATTCTGA CCGTGGGCCC ATTCTGCCTG TTCCATTGGG AATTCGACCA
    GTTCTCAGCA GATATCGCAT CGAGGTCCTG TTTTGGGGTC TGAGGGACCT TAAAAGGGTC
    AACCTGGCTC AGGTGGACCG ACCTCGTGTG GACATTGAGT GTGCAGGGAA AGGAGTTCAG
    TCAGCCCTAA TTCAAAACTA CAAGAAGAAT CCAAACTTCA GCACATTAGT AAAGTGGTTT
    GAAGTGGATC TGCCAGAAAA TGAGCTGCTC CATCCACCAC TTAATATTCG GGTAGTGGAT
    TGCAGGGCAT TCGGACGCTA CATTTTGGTG GGGTCTCATG CTGTGACCAC CCTTCGCAAG
    TTCATCTACA GCCCCCCAGA CAAGACTGCT AACAACTGGG CTCACACAGC TAGACTGGCC
    AATGGCTACC TGGCTCTCAG CAATGGGACG TCTCATTCCC GCCCCCAATC CCGTCCTGTT
    TCTTATCCCT CAGGTGACAT TGTGGTCAAC ATGAGCCCTG AACCTAACAT TAAGAAGATG
    GACACGGTTG TCAAGATTGA AGCTACCACC GATGCGGTTG TTAAAGTTGA CTTGAATGAG
    GATGAGAAGG AGAAAGAAAA GAAGAAGAAG AAGAAGAAGA AAGGAGAAGA AGTGGAGGAA
    GAGGAGCCGG ATGAGAGCAT GCTGGACTGG TGGTCCAAAT ACTTTGCCTC AATAGAGACT
    ATGATGGAGA ATCTAAGAGC TCAGGAGGCC GCTCAGGCAG AGGCAGAGGA AAGAGAAGAT
    TTGGAGATTG CAGCAGAGAG TGCAGGCACC AAACCTAAAG AGAAGAGCAA AGACAAGAAG
    AGCACCAAGG ACAAAAAGAA GAACAACGAT GGCACAGAAA AACGACCTCC AAAACCAAAA
    GTTGATGAAC TCATGGTGTA CAATAAAGAG CTGGAGAGTG AGTTCGGTAG CTTTGAGGAC
    TGGCTTCACA CCTTCAACCT GTACCGAGGA AAGGCTGGAG ATGATGATGA CCACAATGTG
    GTTGATGAGG ACAGAATTGT GGGCAGATTC AAGGGCTCCC TCTGTATGTA TAAACTACCA
    CTGTCTGAGG AGATCACCAG AGAGGCAGGA TTTGATCCAA ACATGGGCAT GTTCCAGAGT
    ATTCCTCACA ATGACCCTAT CAACGTTCTT GTCAGGATTT ATATCATTCG AGCCACAGAC
    CTGCATCCTG CAGATATAAA TGGAAAGGCC GACCCTTATA TAGTCATTAA ATTGGGAAAG
    TCAGACATTC GAGACAAGGA GAACTACATA TCCAAGCAGC TGAATCCTGT CTTTGGAAAG
    TCATTTGATA TAGAGGCCAC GTTCCCAATG GAGTCTATGC TGACTGTGGC AGTGTATGAC
    TGGGATTTAG TCGGAACTGA TGACCTGATT GGTGAGACTA AGATTGATTT GGAGAACAGA
    TACTACAGCA AACACAGAGC CACATGTGGC ATTGCTTCCA ACTACTCTGT CCATGGTTAT
    AATGTATGGA GAGATCCTCA GAAACCAGCT CAGATCCTGG CTAAGTTGTG CAAAGAAGGT
    AAACTGGATG GACCTCACTA TGGACCTGGG GGAAGAGTCA AAGTTGCAAA CCGGATCTTT
    CTTGGGCCGA CAGAAATTGA GGATGAGAGT GGTCTGAAAA AACAAACAGA AGAACACTTG
    GCGCTCACAG TCCTTAGACA TTGGGAGGAA ATCCCACGTG TTGGTTGCAA ACTCATCCCT
    GAGCATGTGG AGACGAGGCC TCTCCTCAAC CCAGACAAGC CAGGCATAGA GCAGGGAAGG
    ATTGAAATGT GGGTGGACAT GTTTCCTATG GATGTCCCAG CACCAGGACC AGCCATTGAT
    ATATCGCCAC GCAAACCAAA GAGATATGAG CTCAGGGTGA TAATATGGAA TACTGACGAG
    GTAATTCTGG AAGACGATGA TTACTTCACA GGGGAAAAGT CCAGTGACAT TTTTGTGAGG
    GGCTGGCTTA AAGGACAGCA GGAGGACAAA CAGGACACCG ATGTACATTA TCACTCTCTA
    ACTGGAGAGG GAAACTTCAA CTGGCGCTTT GTCTTCCCCT TTGACTATCT CATGGCCGAG
    GAGAAGATTG TTATCTCCAA GAAGGAATCA ATGTTTTCCT GGGATGAGAC TGAGTATAAG
    ATCCCTGCTC GACTCACCCT TCAAGTATGG GATGCCGATC ACTTTTCTGC AGATGATTTT
    CTGGGTGCGA TTGAGTTGGA CTTGAATAAG TTTCCGCGTG GGGCAAAAAC AGCCAAGCAG
    TGCTCTCTAG ACATGGTTCT CAAAGAGCAT GAGCTGCCAA CCATCTCCAT CTTCAAACAA
    AAGAGAGTCA AGGGCTGGTG GCCATTTGTG GCCCAAAATG AGAATGATGA ATTTGAGCTA
    ACGGGAAAAG TAGAGGCAGA GCTCCATCTG TTGACAGCAG AGGAGGCAGA GAAGAGTCCA
    GTCGGCCTGG GGAGGAATGA TCCGGAGCCA CTTGAGAAAC CAAATCGTCC AACTACCAGC
    CTGTCGTGGC TGTTGAGTCC GATTCGGGTG ACCAGCATAA TGTGTTGGCG GCAGTATAAG
    TTGGTCACAG TGGTGACTCT GCTCATCATA ACCGGGCTGA TCTTTTTGGG CCTCCTGTTC
    TTTTCTCTGC CTTCAGCTTT CATGCAGAAG CTCTTCAACA TCTCTGGATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42609
    Clone ID Related Accession (Same CDS sequence) XM_009294738.2 , XM_009294738.3
    Accession Version XM_009294738.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6111bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product otoferlin isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009294738.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGGCTTTAG TGGTGCATCT GAAGACTGTC ACGGAGCTCC GTGGGAAGGG GGACCGAATC 
    GCTAAAGTCA CTTTCAGAGG TCTCTCCTTC TTCTCTCGGG TGTTGGAAAA CTGCGAGGAT
    GAAGCTCGAT TTGAGCAGGC GTTCCGATGG CCAATTGGGA GTCAAGTGGA TGGCGACGAG
    ATGCTGGAGA TCCAAGTGTT TAATTACAGC AAAGTCTTCA CTAATAGGTT AATCGGCACA
    TTCCGCATGG TTCTTCAGAA GGTGGTGGAG GAGGGACACC TGGAGGTGTC TGACACACTC
    ATTGACGACA ATAACACAGC CATACAGACA ACCATTTCCA TAGAGATCAA ATATCAGACC
    ATGGACGGGA GTGTAAAGGT GTGGAGCGAT GGTGAATTCC TTGATATTCC AGATGACCTT
    GATGGAACCT TCCAGTTTGA GACAGATAGT CTTCTCTCAG GACGCAGCCA GAGTTCTGGA
    ACTTCACCTG GACGATCCAT TCATGGCATT CCCACATTCC GCAAGGCAGG AAAAGGAGTG
    TTCTCAGCCA TGAAGCTGGG CAAGACCCGC ACCTCTAAAG ATGACCACAA AAAAGGAGAT
    GATGCTGCCA TTCTGGATGC AGAAGATCTG GATCGAAAAG CCATGCGTCT AGGCGGCATT
    CTTGACCCTG ATACAATCTC ACTGGCCTCG GTTACTGCAG TGACCACTAA TGTCTCCAAC
    AAAAGATCAA AGCCTGACAT TAAAATGGAG CCGAGTTCAG GGCGTCCGGT TGATTATCAG
    ATCAGTGTTA CAGTAATCGA GGCCCGTCAG CTGGTCGGAT TGAACATGGA CCCTGTGGTT
    TGCGTGGAAA TTGGAGAGGA GAAGAAGTAC ACGTCAATGA AGGAGTCAAC AAACTGCCCT
    TACTACAATG AGTATTTTGT CTTCGACTTC CACGTGCCTC CAGACGTCAT GTTTGACAAA
    ATTATAAAGA TTTCAGTAAT ACATTCTAAA AACCTTCTGC GAAGCGGCAC CCTCGTGGGC
    ACCTTCAAAC TAGACGTGGG GACTGTTTAC ACACAACCTG AACATCAGTT TCATCACAAA
    TGGGCGATGC TGTCAGACCC TGATGACATC ACGACTGGCT GCAAAGGTTA TGTTAAATGT
    GACATCGCAG TCGTGGGAAA AGGAGACAAC ATCAAGACTC CACACAAGGC CAGTGAAGCA
    GACGAGGATG ACATAGAGGG GAATCTTCTC TTGCCGGAGG GTGTTCCATC AGAGAGACAG
    TGGGCTCGGT TTTATGTTAA GATTTATAGA GCTGAGGGTC TGCCTAAAAT GAACACCAGT
    ATCATGGCCA ACGTGAAGAA AGCCTTTATT GGGGAAAACA GGGACCTTGT GGACCCTTAC
    GTCCTGGTGC AATTTGCAGG GCAAAAGGGC AAAACATCTG TTCAGAAGAG CAGCTACGAG
    CCCATCTGGA ACGAGCAAGT CATCTTCACG GAGATGTTCC CACCTTTGTG TAGGCGACTG
    AAAGTTCAGA TCCGTGATTC AGACAAGGTG AACGATGTTG CCATAGGAAC CCACTTCATT
    GATCTACGAA AGGTTTCCAA TGATGGAGAC AAAGGTTTCT TGCCCACTAT GGGTCCGGCT
    TGGGTGAATA TGTATGGGTC GACCCGTAAC TACACCCTGA TGGACGAACA CCAGGACCTG
    AATGAGGGGT TGGGGGAAGG GGTGTCCTTC AGAGCACGTC TGCTCATTAG TATCGCGGTG
    GAGATTCTAG ACACCACCTC TGCTGAGATC ATGAGCTCCA CTGAAGTACA CATGGAGCCG
    GTGTCCAACA TCTCAGAGAG TGCTACAGGA AAGATGGAAG AATTTTTCCT TTTCGGGTCA
    TTCTTGGAGG CCACAATGAT AGACAGAAAA ATAGGAGATA AACCCATCAG CTTTGAAGTC
    ACCATCGGTA ACTATGGTAA CCAGATTGAT GGTGTGAGCA AGCCTGCATT AGCGAAGAAG
    AAGAAGGAGG GTGGAGGAGA GAGTGAAGAA GAGGAGTCTG AGCTTATCCA CAACTCCAGC
    GAGGAAGAGG CAGAGGATGA TGGAGATCTG ACCTCTGTAC CGTCTACACC TCCTATGAAG
    CCTGTTATCA CCGACAGAAA TTACTTCCAC TTGCCTTACT TTGAAAAGAA ACCCTGCATT
    TACATCAAGA GCTGGTGGCA GGACCAGCGG AGACGTCTCT ACAACTCCAA CATAATGGAC
    AAGATAGCTG ATAAACTGGA GGAAGGTCTG AATGATGTTC AAGAGATCAT AAAAACAGAG
    AAGGCATATC CAGAACGAAG ACTAAGAGGT GTATTAGAAG AACTTAGCAC AAGCTGCAGC
    CGGTTTGTTA CACTTGCAAA CAAGGATCAG AATCTTTCTG GAAGAACCAA GCTGGACAGA
    GAGAGGCTGA AGTCCTGTAT GAGAGAAATG GAGAGTATGG GTCAGCAGGC GAAGACCATC
    CGCACACAAG TGAAAAGAAA CACAGTTCGA GATAAACTCA AGCTAGTGTT GAATTTCCTT
    CAGAGGCTTC GATTCTTAGC AGACGAGCCC CAGCACAGCA TCCCTGATGT GTTCATATGG
    ATGATTAGCA ACAACAAACG CATCGCATAC GCCCGCATAC CCTCCAAAGA CATCCTCTAC
    TCAATTGTCG ATGAAGAGAT GGGCAAAGAC TGTGGAAAAG TTAAAGCTGT TTTTCTCAGG
    CTGCCTGGAA AGAAAGGTTT TGGGCCTGCT GGCTGGACAG TTCAGGCTAA ATTAGAGATG
    TATTTGTGGC TGGGCCTGAA TAAACAGAGG AAAGACTTCC TGATTGGCCT CCCTAGTGGC
    TTTGAAGAAA ACAAGGCAGT AAAGGGAATT GGCATACAAG CTGTCCCTCC CATCAGCCTA
    GTTTATAACA TGAAGCAGGT GTTTCAGCTG AGGGCCCACA TGTATCAAGC TCGCAGTCTG
    TTTGCTGCGG ACACTAGTGG CCTGTCTGAT CCATTTGCAA GGGTTTTCTT CTCCACCCAC
    AGCCAGGTCA CGGAGGTCCT CAGTGAGACT CTCTGTCCAA CATGGGATCA GCTGCTTGTG
    TTTGATAATG TTGAACTTTA CGGTGAGGCC GGTGAACTCC GTGATGACCC TCCAATCATT
    GTCATTGAGT TGTATGATCA AGACACTGTG GGGAAAGCTG AGTTTATTGG CCGAACATTT
    GCAAAACCAC TTACTAAGAT GGTTGATGAA CACTACGGCC CACCGCGGTT TCCTCCCCAG
    CTCGAGTATT ATCAGATCTA CCGAGGAAAC TGTGCTGCGG GAGATCTATT GGCTGCTTTT
    GAGCTTCTAC AGATTCCTTA TGACGATGAG GAGATCAGAA GAGCTCTTAT TGCTGTCCAT
    GACTTTGCTG TGCCTCAGAT CAAGATTGGT CCAGCAGGGC GGGCAGCACT TCCTCCAATC
    GATGGACCGA CCGATTCTGA CCGTGGGCCC ATTCTGCCTG TTCCATTGGG AATTCGACCA
    GTTCTCAGCA GATATCGCAT CGAGGTCCTG TTTTGGGGTC TGAGGGACCT TAAAAGGGTC
    AACCTGGCTC AGGTGGACCG ACCTCGTGTG GACATTGAGT GTGCAGGGAA AGGAGTTCAG
    TCAGCCCTAA TTCAAAACTA CAAGAAGAAT CCAAACTTCA GCACATTAGT AAAGTGGTTT
    GAAGTGGATC TGCCAGAAAA TGAGCTGCTC CATCCACCAC TTAATATTCG GGTAGTGGAT
    TGCAGGGCAT TCGGACGCTA CATTTTGGTG GGGTCTCATG CTGTGACCAC CCTTCGCAAG
    TTCATCTACA GCCCCCCAGA CAAGACTGCT AACAACTGGG CTCACACAGC TAGACTGGCC
    AATGGCTACC TGGCTCTCAG CAATGGGACG TCTCATTCCC GCCCCCAATC CCGTCCTGTT
    TCTTATCCCT CAGGTGACAT TGTGGTCAAC ATGAGCCCTG AACCTAACAT TAAGAAGATG
    GACACGGTTG TCAAGATTGA AGCTACCACC GATGCGGTTG TTAAAGTTGA CTTGAATGAG
    GATGAGAAGG AGAAAGAAAA GAAGAAGAAG AAGAAGAAGA AAGGAGAAGA AGTGGAGGAA
    GAGGAGCCGG ATGAGAGCAT GCTGGACTGG TGGTCCAAAT ACTTTGCCTC AATAGAGACT
    ATGATGGAGA ATCTAAGAGC TCAGGAGGCC GCTCAGGCAG AGGCAGAGGA AAGAGAAGAT
    TTGGAGATTG CAGCAGAGAG TGCAGGCACC AAACCTAAAG AGAAGAGCAA AGACAAGAAG
    AGCACCAAGG ACAAAAAGAA GAACAACGAT GGCACAGAAA AACGACCTCC AAAACCAAAA
    GTTGATGAAC TCATGGTGTA CAATAAAGAG CTGGAGAGTG AGTTCGGTAG CTTTGAGGAC
    TGGCTTCACA CCTTCAACCT GTACCGAGGA AAGGCTGGAG ATGATGATGA CCACAATGTG
    GTTGATGAGG ACAGAATTGT GGGCAGATTC AAGGGCTCCC TCTGTATGTA TAAACTACCA
    CTGTCTGAGG AGATCACCAG AGAGGCAGGA TTTGATCCAA ACATGGGCAT GTTCCAGAGT
    ATTCCTCACA ATGACCCTAT CAACGTTCTT GTCAGGATTT ATATCATTCG AGCCACAGAC
    CTGCATCCTG CAGATATAAA TGGAAAGGCC GACCCTTATA TAGTCATTAA ATTGGGAAAG
    TCAGACATTC GAGACAAGGA GAACTACATA TCCAAGCAGC TGAATCCTGT CTTTGGAAAG
    TCATTTGATA TAGAGGCCAC GTTCCCAATG GAGTCTATGC TGACTGTGGC AGTGTATGAC
    TGGGATTTAG TCGGAACTGA TGACCTGATT GGTGAGACTA AGATTGATTT GGAGAACAGA
    TACTACAGCA AACACAGAGC CACATGTGGC ATTGCTTCCA ACTACTCTGT CCATGGTTAT
    AATGTATGGA GAGATCCTCA GAAACCAGCT CAGATCCTGG CTAAGTTGTG CAAAGAAGGT
    AAACTGGATG GACCTCACTA TGGACCTGGG GGAAGAGTCA AAGTTGCAAA CCGGATCTTT
    CTTGGGCCGA CAGAAATTGA GGATGAGAGT GGTCTGAAAA AACAAACAGA AGAACACTTG
    GCGCTCACAG TCCTTAGACA TTGGGAGGAA ATCCCACGTG TTGGTTGCAA ACTCATCCCT
    GAGCATGTGG AGACGAGGCC TCTCCTCAAC CCAGACAAGC CAGGCATAGA GCAGGGAAGG
    ATTGAAATGT GGGTGGACAT GTTTCCTATG GATGTCCCAG CACCAGGACC AGCCATTGAT
    ATATCGCCAC GCAAACCAAA GAGATATGAG CTCAGGGTGA TAATATGGAA TACTGACGAG
    GTAATTCTGG AAGACGATGA TTACTTCACA GGGGAAAAGT CCAGTGACAT TTTTGTGAGG
    GGCTGGCTTA AAGGACAGCA GGAGGACAAA CAGGACACCG ATGTACATTA TCACTCTCTA
    ACTGGAGAGG GAAACTTCAA CTGGCGCTTT GTCTTCCCCT TTGACTATCT CATGGCCGAG
    GAGAAGATTG TTATCTCCAA GAAGGAATCA ATGTTTTCCT GGGATGAGAC TGAGTATAAG
    ATCCCTGCTC GACTCACCCT TCAAGTATGG GATGCCGATC ACTTTTCTGC AGATGATTTT
    CTGGGTGCGA TTGAGTTGGA CTTGAATAAG TTTCCGCGTG GGGCAAAAAC AGCCAAGCAG
    TGCTCTCTAG ACATGGTTCT CAAAGAGCAT GAGCTGCCAA CCATCTCCAT CTTCAAACAA
    AAGAGAGTCA AGGGCTGGTG GCCATTTGTG GCCCAAAATG AGAATGATGA ATTTGAGCTA
    ACGGGAAAAG TAGAGGCAGA GCTCCATCTG TTGACAGCAG AGGAGGCAGA GAAGAGTCCA
    GTCGGCCTGG GGAGGAATGA TCCGGAGCCA CTTGAGAAAC CAAATCGTCC AACTACCAGC
    CTGTCGTGGC TGTTGAGTCC GATTCGGGTG ACCAGCATAA TGTGTTGGCG GCAGTATAAG
    TTGGTCACAG TGGTGACTCT GCTCATCATA ACCGGGCTGA TCTTTTTGGG CCTCCTGTTC
    TTTTCTCTGC CTTCAGCTTT CATGCAGAAG CTCTTCAACA TCTCTGGATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009293013.1
    CDS301..6411
    Translation

    Target ORF information:

    RefSeq Version XM_009294738.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio otoferlin a (otofa), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009294738.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGGCTTTAG TGGTGCATCT GAAGACTGTC ACGGAGCTCC GTGGGAAGGG GGACCGAATC 
    GCTAAAGTCA CTTTCAGAGG TCTCTCCTTC TTCTCTCGGG TGTTGGAAAA CTGCGAGGAT
    GAAGCTCGAT TTGAGCAGGC GTTCCGATGG CCAATTGGGA GTCAAGTGGA TGGCGACGAG
    ATGCTGGAGA TCCAAGTGTT TAATTACAGC AAAGTCTTCA CTAATAGGTT AATCGGCACA
    TTCCGCATGG TTCTTCAGAA GGTGGTGGAG GAGGGACACC TGGAGGTGTC TGACACACTC
    ATTGACGACA ATAACACAGC CATACAGACA ACCATTTCCA TAGAGATCAA ATATCAGACC
    ATGGACGGGA GTGTAAAGGT GTGGAGCGAT GGTGAATTCC TTGATATTCC AGATGACCTT
    GATGGAACCT TCCAGTTTGA GACAGATAGT CTTCTCTCAG GACGCAGCCA GAGTTCTGGA
    ACTTCACCTG GACGATCCAT TCATGGCATT CCCACATTCC GCAAGGCAGG AAAAGGAGTG
    TTCTCAGCCA TGAAGCTGGG CAAGACCCGC ACCTCTAAAG ATGACCACAA AAAAGGAGAT
    GATGCTGCCA TTCTGGATGC AGAAGATCTG GATCGAAAAG CCATGCGTCT AGGCGGCATT
    CTTGACCCTG ATACAATCTC ACTGGCCTCG GTTACTGCAG TGACCACTAA TGTCTCCAAC
    AAAAGATCAA AGCCTGACAT TAAAATGGAG CCGAGTTCAG GGCGTCCGGT TGATTATCAG
    ATCAGTGTTA CAGTAATCGA GGCCCGTCAG CTGGTCGGAT TGAACATGGA CCCTGTGGTT
    TGCGTGGAAA TTGGAGAGGA GAAGAAGTAC ACGTCAATGA AGGAGTCAAC AAACTGCCCT
    TACTACAATG AGTATTTTGT CTTCGACTTC CACGTGCCTC CAGACGTCAT GTTTGACAAA
    ATTATAAAGA TTTCAGTAAT ACATTCTAAA AACCTTCTGC GAAGCGGCAC CCTCGTGGGC
    ACCTTCAAAC TAGACGTGGG GACTGTTTAC ACACAACCTG AACATCAGTT TCATCACAAA
    TGGGCGATGC TGTCAGACCC TGATGACATC ACGACTGGCT GCAAAGGTTA TGTTAAATGT
    GACATCGCAG TCGTGGGAAA AGGAGACAAC ATCAAGACTC CACACAAGGC CAGTGAAGCA
    GACGAGGATG ACATAGAGGG GAATCTTCTC TTGCCGGAGG GTGTTCCATC AGAGAGACAG
    TGGGCTCGGT TTTATGTTAA GATTTATAGA GCTGAGGGTC TGCCTAAAAT GAACACCAGT
    ATCATGGCCA ACGTGAAGAA AGCCTTTATT GGGGAAAACA GGGACCTTGT GGACCCTTAC
    GTCCTGGTGC AATTTGCAGG GCAAAAGGGC AAAACATCTG TTCAGAAGAG CAGCTACGAG
    CCCATCTGGA ACGAGCAAGT CATCTTCACG GAGATGTTCC CACCTTTGTG TAGGCGACTG
    AAAGTTCAGA TCCGTGATTC AGACAAGGTG AACGATGTTG CCATAGGAAC CCACTTCATT
    GATCTACGAA AGGTTTCCAA TGATGGAGAC AAAGGTTTCT TGCCCACTAT GGGTCCGGCT
    TGGGTGAATA TGTATGGGTC GACCCGTAAC TACACCCTGA TGGACGAACA CCAGGACCTG
    AATGAGGGGT TGGGGGAAGG GGTGTCCTTC AGAGCACGTC TGCTCATTAG TATCGCGGTG
    GAGATTCTAG ACACCACCTC TGCTGAGATC ATGAGCTCCA CTGAAGTACA CATGGAGCCG
    GTGTCCAACA TCTCAGAGAG TGCTACAGGA AAGATGGAAG AATTTTTCCT TTTCGGGTCA
    TTCTTGGAGG CCACAATGAT AGACAGAAAA ATAGGAGATA AACCCATCAG CTTTGAAGTC
    ACCATCGGTA ACTATGGTAA CCAGATTGAT GGTGTGAGCA AGCCTGCATT AGCGAAGAAG
    AAGAAGGAGG GTGGAGGAGA GAGTGAAGAA GAGGAGTCTG AGCTTATCCA CAACTCCAGC
    GAGGAAGAGG CAGAGGATGA TGGAGATCTG ACCTCTGTAC CGTCTACACC TCCTATGAAG
    CCTGTTATCA CCGACAGAAA TTACTTCCAC TTGCCTTACT TTGAAAAGAA ACCCTGCATT
    TACATCAAGA GCTGGTGGCA GGACCAGCGG AGACGTCTCT ACAACTCCAA CATAATGGAC
    AAGATAGCTG ATAAACTGGA GGAAGGTCTG AATGATGTTC AAGAGATCAT AAAAACAGAG
    AAGGCATATC CAGAACGAAG ACTAAGAGGT GTATTAGAAG AACTTAGCAC AAGCTGCAGC
    CGGTTTGTTA CACTTGCAAA CAAGGATCAG AATCTTTCTG GAAGAACCAA GCTGGACAGA
    GAGAGGCTGA AGTCCTGTAT GAGAGAAATG GAGAGTATGG GTCAGCAGGC GAAGACCATC
    CGCACACAAG TGAAAAGAAA CACAGTTCGA GATAAACTCA AGCTAGTGTT GAATTTCCTT
    CAGAGGCTTC GATTCTTAGC AGACGAGCCC CAGCACAGCA TCCCTGATGT GTTCATATGG
    ATGATTAGCA ACAACAAACG CATCGCATAC GCCCGCATAC CCTCCAAAGA CATCCTCTAC
    TCAATTGTCG ATGAAGAGAT GGGCAAAGAC TGTGGAAAAG TTAAAGCTGT TTTTCTCAGG
    CTGCCTGGAA AGAAAGGTTT TGGGCCTGCT GGCTGGACAG TTCAGGCTAA ATTAGAGATG
    TATTTGTGGC TGGGCCTGAA TAAACAGAGG AAAGACTTCC TGATTGGCCT CCCTAGTGGC
    TTTGAAGAAA ACAAGGCAGT AAAGGGAATT GGCATACAAG CTGTCCCTCC CATCAGCCTA
    GTTTATAACA TGAAGCAGGT GTTTCAGCTG AGGGCCCACA TGTATCAAGC TCGCAGTCTG
    TTTGCTGCGG ACACTAGTGG CCTGTCTGAT CCATTTGCAA GGGTTTTCTT CTCCACCCAC
    AGCCAGGTCA CGGAGGTCCT CAGTGAGACT CTCTGTCCAA CATGGGATCA GCTGCTTGTG
    TTTGATAATG TTGAACTTTA CGGTGAGGCC GGTGAACTCC GTGATGACCC TCCAATCATT
    GTCATTGAGT TGTATGATCA AGACACTGTG GGGAAAGCTG AGTTTATTGG CCGAACATTT
    GCAAAACCAC TTACTAAGAT GGTTGATGAA CACTACGGCC CACCGCGGTT TCCTCCCCAG
    CTCGAGTATT ATCAGATCTA CCGAGGAAAC TGTGCTGCGG GAGATCTATT GGCTGCTTTT
    GAGCTTCTAC AGATTCCTTA TGACGATGAG GAGATCAGAA GAGCTCTTAT TGCTGTCCAT
    GACTTTGCTG TGCCTCAGAT CAAGATTGGT CCAGCAGGGC GGGCAGCACT TCCTCCAATC
    GATGGACCGA CCGATTCTGA CCGTGGGCCC ATTCTGCCTG TTCCATTGGG AATTCGACCA
    GTTCTCAGCA GATATCGCAT CGAGGTCCTG TTTTGGGGTC TGAGGGACCT TAAAAGGGTC
    AACCTGGCTC AGGTGGACCG ACCTCGTGTG GACATTGAGT GTGCAGGGAA AGGAGTTCAG
    TCAGCCCTAA TTCAAAACTA CAAGAAGAAT CCAAACTTCA GCACATTAGT AAAGTGGTTT
    GAAGTGGATC TGCCAGAAAA TGAGCTGCTC CATCCACCAC TTAATATTCG GGTAGTGGAT
    TGCAGGGCAT TCGGACGCTA CATTTTGGTG GGGTCTCATG CTGTGACCAC CCTTCGCAAG
    TTCATCTACA GCCCCCCAGA CAAGACTGCT AACAACTGGG CTCACACAGC TAGACTGGCC
    AATGGCTACC TGGCTCTCAG CAATGGGACG TCTCATTCCC GCCCCCAATC CCGTCCTGTT
    TCTTATCCCT CAGGTGACAT TGTGGTCAAC ATGAGCCCTG AACCTAACAT TAAGAAGATG
    GACACGGTTG TCAAGATTGA AGCTACCACC GATGCGGTTG TTAAAGTTGA CTTGAATGAG
    GATGAGAAGG AGAAAGAAAA GAAGAAGAAG AAGAAGAAGA AAGGAGAAGA AGTGGAGGAA
    GAGGAGCCGG ATGAGAGCAT GCTGGACTGG TGGTCCAAAT ACTTTGCCTC AATAGAGACT
    ATGATGGAGA ATCTAAGAGC TCAGGAGGCC GCTCAGGCAG AGGCAGAGGA AAGAGAAGAT
    TTGGAGATTG CAGCAGAGAG TGCAGGCACC AAACCTAAAG AGAAGAGCAA AGACAAGAAG
    AGCACCAAGG ACAAAAAGAA GAACAACGAT GGCACAGAAA AACGACCTCC AAAACCAAAA
    GTTGATGAAC TCATGGTGTA CAATAAAGAG CTGGAGAGTG AGTTCGGTAG CTTTGAGGAC
    TGGCTTCACA CCTTCAACCT GTACCGAGGA AAGGCTGGAG ATGATGATGA CCACAATGTG
    GTTGATGAGG ACAGAATTGT GGGCAGATTC AAGGGCTCCC TCTGTATGTA TAAACTACCA
    CTGTCTGAGG AGATCACCAG AGAGGCAGGA TTTGATCCAA ACATGGGCAT GTTCCAGAGT
    ATTCCTCACA ATGACCCTAT CAACGTTCTT GTCAGGATTT ATATCATTCG AGCCACAGAC
    CTGCATCCTG CAGATATAAA TGGAAAGGCC GACCCTTATA TAGTCATTAA ATTGGGAAAG
    TCAGACATTC GAGACAAGGA GAACTACATA TCCAAGCAGC TGAATCCTGT CTTTGGAAAG
    TCATTTGATA TAGAGGCCAC GTTCCCAATG GAGTCTATGC TGACTGTGGC AGTGTATGAC
    TGGGATTTAG TCGGAACTGA TGACCTGATT GGTGAGACTA AGATTGATTT GGAGAACAGA
    TACTACAGCA AACACAGAGC CACATGTGGC ATTGCTTCCA ACTACTCTGT CCATGGTTAT
    AATGTATGGA GAGATCCTCA GAAACCAGCT CAGATCCTGG CTAAGTTGTG CAAAGAAGGT
    AAACTGGATG GACCTCACTA TGGACCTGGG GGAAGAGTCA AAGTTGCAAA CCGGATCTTT
    CTTGGGCCGA CAGAAATTGA GGATGAGAGT GGTCTGAAAA AACAAACAGA AGAACACTTG
    GCGCTCACAG TCCTTAGACA TTGGGAGGAA ATCCCACGTG TTGGTTGCAA ACTCATCCCT
    GAGCATGTGG AGACGAGGCC TCTCCTCAAC CCAGACAAGC CAGGCATAGA GCAGGGAAGG
    ATTGAAATGT GGGTGGACAT GTTTCCTATG GATGTCCCAG CACCAGGACC AGCCATTGAT
    ATATCGCCAC GCAAACCAAA GAGATATGAG CTCAGGGTGA TAATATGGAA TACTGACGAG
    GTAATTCTGG AAGACGATGA TTACTTCACA GGGGAAAAGT CCAGTGACAT TTTTGTGAGG
    GGCTGGCTTA AAGGACAGCA GGAGGACAAA CAGGACACCG ATGTACATTA TCACTCTCTA
    ACTGGAGAGG GAAACTTCAA CTGGCGCTTT GTCTTCCCCT TTGACTATCT CATGGCCGAG
    GAGAAGATTG TTATCTCCAA GAAGGAATCA ATGTTTTCCT GGGATGAGAC TGAGTATAAG
    ATCCCTGCTC GACTCACCCT TCAAGTATGG GATGCCGATC ACTTTTCTGC AGATGATTTT
    CTGGGTGCGA TTGAGTTGGA CTTGAATAAG TTTCCGCGTG GGGCAAAAAC AGCCAAGCAG
    TGCTCTCTAG ACATGGTTCT CAAAGAGCAT GAGCTGCCAA CCATCTCCAT CTTCAAACAA
    AAGAGAGTCA AGGGCTGGTG GCCATTTGTG GCCCAAAATG AGAATGATGA ATTTGAGCTA
    ACGGGAAAAG TAGAGGCAGA GCTCCATCTG TTGACAGCAG AGGAGGCAGA GAAGAGTCCA
    GTCGGCCTGG GGAGGAATGA TCCGGAGCCA CTTGAGAAAC CAAATCGTCC AACTACCAGC
    CTGTCGTGGC TGTTGAGTCC GATTCGGGTG ACCAGCATAA TGTGTTGGCG GCAGTATAAG
    TTGGTCACAG TGGTGACTCT GCTCATCATA ACCGGGCTGA TCTTTTTGGG CCTCCTGTTC
    TTTTCTCTGC CTTCAGCTTT CATGCAGAAG CTCTTCAACA TCTCTGGATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42610
    Clone ID Related Accession (Same CDS sequence) XM_009294739.2 , XM_009294739.3
    Accession Version XM_009294739.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6069bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product otoferlin isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009294739.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGGCTTTAG TGGTGCATCT GAAGACTGTC ACGGAGCTCC GTGGGAAGGG GGACCGAATC 
    GCTAAAGTCA CTTTCAGAGG TCTCTCCTTC TTCTCTCGGG TGTTGGAAAA CTGCGAGGAT
    GAAGCTCGAT TTGAGCAGGC GTTCCGATGG CCAATTGGGA GTCAAGTGGA TGGCGACGAG
    ATGCTGGAGA TCCAAGTGTT TAATTACAGC AAAGTCTTCA CTAATAGGTT AATCGGCACA
    TTCCGCATGG TTCTTCAGAA GGTGGTGGAG GAGGGACACC TGGAGGTGTC TGACACACTC
    ATTGACGACA ATAACACAGC CATACAGACA ACCATTTCCA TAGAGATCAA ATATCAGACC
    ATGGACGGGA GTGTAAAGGT GTGGAGCGAT GGTGAATTCC TTGATATTCC AGATGACCTT
    GATGGAACCT TCCAGTTTGA GACAGATAGT CTTCTCTCAG GACGCAGCCA GAGTTCTGGA
    ACTTCACCTG GACGATCCAT TCATGGCATT CCCACATTCC GCAAGGCAGG AAAAGGAGTG
    TTCTCAGCCA TGAAGCTGGG CAAGACCCGC ACCTCTAAAG ATGACCACAA AAAAGGAGAT
    GATGCTGCCA TTCTGGATGC AGAAGATCTG GATCGAAAAG CCATGCGTCT AGGCGGCATT
    CTTGACCCTG ATACAATCTC ACTGGCCTCG GTTACTGCAG TGACCACTAA TGTCTCCAAC
    AAAAGATCAA AGCCTGACAT TAAAATGGAG CCGAGTTCAG GGCGTCCGGT TGATTATCAG
    ATCAGTGTTA CAGTAATCGA GGCCCGTCAG CTGGTCGGAT TGAACATGGA CCCTGTGGTT
    TGCGTGGAAA TTGGAGAGGA GAAGAAGTAC ACGTCAATGA AGGAGTCAAC AAACTGCCCT
    TACTACAATG AGTATTTTGT CTTCGACTTC CACGTGCCTC CAGACGTCAT GTTTGACAAA
    ATTATAAAGA TTTCAGTAAT ACATTCTAAA AACCTTCTGC GAAGCGGCAC CCTCGTGGGC
    ACCTTCAAAC TAGACGTGGG GACTGTTTAC ACACAACCTG AACATCAGTT TCATCACAAA
    TGGGCGATGC TGTCAGACCC TGATGACATC ACGACTGGCT GCAAAGGTTA TGTTAAATGT
    GACATCGCAG TCGTGGGAAA AGGAGACAAC ATCAAGACTC CACACAAGGC CAGTGAAGCA
    GACGAGGATG ACATAGAGGG GAATCTTCTC TTGCCGGAGG GTGTTCCATC AGAGAGACAG
    TGGGCTCGGT TTTATGTTAA GATTTATAGA GCTGAGGGTC TGCCTAAAAT GAACACCAGT
    ATCATGGCCA ACGTGAAGAA AGCCTTTATT GGGGAAAACA GGGACCTTGT GGACCCTTAC
    GTCCTGGTGC AATTTGCAGG GCAAAAGGGC AAAACATCTG TTCAGAAGAG CAGCTACGAG
    CCCATCTGGA ACGAGCAAGT CATCTTCACG GAGATGTTCC CACCTTTGTG TAGGCGACTG
    AAAGTTCAGA TCCGTGATTC AGACAAGGTG AACGATGTTG CCATAGGAAC CCACTTCATT
    GATCTACGAA AGGTTTCCAA TGATGGAGAC AAAGGTTTCT TGCCCACTAT GGGTCCGGCT
    TGGGTGAATA TGTATGGGTC GACCCGTAAC TACACCCTGA TGGACGAACA CCAGGACCTG
    AATGAGGGGT TGGGGGAAGG GGTGTCCTTC AGAGCACGTC TGCTCATTAG TATCGCGGTG
    GAGATTCTAG ACACCACCTC TGCTGAGATC ATGAGCTCCA CTGAAGTACA CATGGAGCCG
    GTGTCCAACA TCTCAGAGAG TGCTACAGGA AAGATGGAAG AATTTTTCCT TTTCGGGTCA
    TTCTTGGAGG CCACAATGAT AGACAGAAAA ATAGGAGATA AACCCATCAG CTTTGAAGTC
    ACCATCGGTA ACTATGGTAA CCAGATTGAT GGTGTGAGCA AGCCTGCATT AGCGAAGAAG
    AAGAAGGAGG GTGGAGGAGA GAGTGAAGAA GAGGAGTCTG AGCTTATCCA CAACTCCAGC
    GAGGAAGAGG CAGAGGATGA TGGAGATCTG ACCTCTGTAC CGTCTACACC TCCTATGAAG
    CCTGTTATCA CCGACAGAAA TTACTTCCAC TTGCCTTACT TTGAAAAGAA ACCCTGCATT
    TACATCAAGA GCTGGTGGCA GGACCAGCGG AGACGTCTCT ACAACTCCAA CATAATGGAC
    AAGATAGCTG ATAAACTGGA GGAAGGTCTG AATGATGTTC AAGAGATCAT AAAAACAGAG
    AAGGCATATC CAGAACGAAG ACTAAGAGGT GTATTAGAAG AACTTAGCAC AAGCTGCAGC
    CGGTTTGTTA CACTTGCAAA CAAGGATCAG AATCTTTCTG GAAGAACCAA GCTGGACAGA
    GAGAGGCTGA AGTCCTGTAT GAGAGAAATG GAGAGTATGG GTCAGCAGGC GAAGACCATC
    CGCACACAAG TGAAAAGAAA CACAGTTCGA GATAAACTCA AGCTAGTGTT GAATTTCCTT
    CAGAGGCTTC GATTCTTAGC AGACGAGCCC CAGCACAGCA TCCCTGATGT GTTCATATGG
    ATGATTAGCA ACAACAAACG CATCGCATAC GCCCGCATAC CCTCCAAAGA CATCCTCTAC
    TCAATTGTCG ATGAAGAGAT GGGCAAAGAC TGTGGAAAAG TTAAAGCTGT TTTTCTCAGG
    CTGCCTGGAA AGAAAGGTTT TGGGCCTGCT GGCTGGACAG TTCAGGCTAA ATTAGAGATG
    TATTTGTGGC TGGGCCTGAA TAAACAGAGG AAAGACTTCC TGATTGGCCT CCCTAGTGGC
    TTTGAAGAAA ACAAGGCAGT AAAGGGAATT GGCATACAAG CTGTCCCTCC CATCAGCCTA
    GTTTATAACA TGAAGCAGGT GTTTCAGCTG AGGGCCCACA TGTATCAAGC TCGCAGTCTG
    TTTGCTGCGG ACACTAGTGG CCTGTCTGAT CCATTTGCAA GGGTTTTCTT CTCCACCCAC
    AGCCAGGTCA CGGAGGTCCT CAGTGAGACT CTCTGTCCAA CATGGGATCA GCTGCTTGTG
    TTTGATAATG TTGAACTTTA CGGTGAGGCC GGTGAACTCC GTGATGACCC TCCAATCATT
    GTCATTGAGT TGTATGATCA AGACACTGTG GGGAAAGCTG AGTTTATTGG CCGAACATTT
    GCAAAACCAC TTACTAAGAT GGTTGATGAA CACTACGGCC CACCGCGGTT TCCTCCCCAG
    CTCGAGTATT ATCAGATCTA CCGAGGAAAC TGTGCTGCGG GAGATCTATT GGCTGCTTTT
    GAGCTTCTAC AGATTGGTCC AGCAGGGCGG GCAGCACTTC CTCCAATCGA TGGACCGACC
    GATTCTGACC GTGGGCCCAT TCTGCCTGTT CCATTGGGAA TTCGACCAGT TCTCAGCAGA
    TATCGCATCG AGGTCCTGTT TTGGGGTCTG AGGGACCTTA AAAGGGTCAA CCTGGCTCAG
    GTGGACCGAC CTCGTGTGGA CATTGAGTGT GCAGGGAAAG GAGTTCAGTC AGCCCTAATT
    CAAAACTACA AGAAGAATCC AAACTTCAGC ACATTAGTAA AGTGGTTTGA AGTGGATCTG
    CCAGAAAATG AGCTGCTCCA TCCACCACTT AATATTCGGG TAGTGGATTG CAGGGCATTC
    GGACGCTACA TTTTGGTGGG GTCTCATGCT GTGACCACCC TTCGCAAGTT CATCTACAGC
    CCCCCAGACA AGACTGCTAA CAACTGGGCT CACACAGCTA GACTGGCCAA TGGCTACCTG
    GCTCTCAGCA ATGGGACGTC TCATTCCCGC CCCCAATCCC GTCCTGTTTC TTATCCCTCA
    GGTGACATTG TGGTCAACAT GAGCCCTGAA CCTAACATTA AGAAGATGGA CACGGTTGTC
    AAGATTGAAG CTACCACCGA TGCGGTTGTT AAAGTTGACT TGAATGAGGA TGAGAAGGAG
    AAAGAAAAGA AGAAGAAGAA GAAGAAGAAA GGAGAAGAAG TGGAGGAAGA GGAGCCGGAT
    GAGAGCATGC TGGACTGGTG GTCCAAATAC TTTGCCTCAA TAGAGACTAT GATGGAGAAT
    CTAAGAGCTC AGGAGGCCGC TCAGGCAGAG GCAGAGGAAA GAGAAGATTT GGAGATTGCA
    GCAGAGAGTG CAGAGATCAA AGCTGATGAC TTTCCTATGA AAGGCACCAA ACCTAAAGAG
    AAGAGCAAAG ACAAGAAGAG CACCAAGGAC AAAAAGAAGA ACAACGATGG CACAGAAAAA
    CGACCTCCAA AACCAAAAGT TGATGAACTC ATGGTGTACA ATAAAGAGCT GGAGAGTGAG
    TTCGGTAGCT TTGAGGACTG GCTTCACACC TTCAACCTGT ACCGAGGAAA GGCTGGAGAT
    GATGATGACC ACAATGTGGT TGATGAGGAC AGAATTGTGG GCAGATTCAA GGGCTCCCTC
    TGTATGTATA AACTACCACT GTCTGAGGAG ATCACCAGAG AGGCAGGATT TGATCCAAAC
    ATGGGCATGT TCCAGAGTAT TCCTCACAAT GACCCTATCA ACGTTCTTGT CAGGATTTAT
    ATCATTCGAG CCACAGACCT GCATCCTGCA GATATAAATG GAAAGGCCGA CCCTTATATA
    GTCATTAAAT TGGGAAAGTC AGACATTCGA GACAAGGAGA ACTACATATC CAAGCAGCTG
    AATCCTGTCT TTGGAAAGTC ATTTGATATA GAGGCCACGT TCCCAATGGA GTCTATGCTG
    ACTGTGGCAG TGTATGACTG GGATTTAGTC GGAACTGATG ACCTGATTGG TGAGACTAAG
    ATTGATTTGG AGAACAGATA CTACAGCAAA CACAGAGCCA CATGTGGCAT TGCTTCCAAC
    TACTCTGTCC ATGGTTATAA TGTATGGAGA GATCCTCAGA AACCAGCTCA GATCCTGGCT
    AAGTTGTGCA AAGAAGGTAA ACTGGATGGA CCTCACTATG GACCTGGGGG AAGAGTCAAA
    GTTGCAAACC GGATCTTTCT TGGGCCGACA GAAATTGAGG ATGAGAGTGG TCTGAAAAAA
    CAAACAGAAG AACACTTGGC GCTCACAGTC CTTAGACATT GGGAGGAAAT CCCACGTGTT
    GGTTGCAAAC TCATCCCTGA GCATGTGGAG ACGAGGCCTC TCCTCAACCC AGACAAGCCA
    GGCATAGAGC AGGGAAGGAT TGAAATGTGG GTGGACATGT TTCCTATGGA TGTCCCAGCA
    CCAGGACCAG CCATTGATAT ATCGCCACGC AAACCAAAGA GATATGAGCT CAGGGTGATA
    ATATGGAATA CTGACGAGGT AATTCTGGAA GACGATGATT ACTTCACAGG GGAAAAGTCC
    AGTGACATTT TTGTGAGGGG CTGGCTTAAA GGACAGCAGG AGGACAAACA GGACACCGAT
    GTACATTATC ACTCTCTAAC TGGAGAGGGA AACTTCAACT GGCGCTTTGT CTTCCCCTTT
    GACTATCTCA TGGCCGAGGA GAAGATTGTT ATCTCCAAGA AGGAATCAAT GTTTTCCTGG
    GATGAGACTG AGTATAAGAT CCCTGCTCGA CTCACCCTTC AAGTATGGGA TGCCGATCAC
    TTTTCTGCAG ATGATTTTCT GGGTGCGATT GAGTTGGACT TGAATAAGTT TCCGCGTGGG
    GCAAAAACAG CCAAGCAGTG CTCTCTAGAC ATGGTTCTCA AAGAGCATGA GCTGCCAACC
    ATCTCCATCT TCAAACAAAA GAGAGTCAAG GGCTGGTGGC CATTTGTGGC CCAAAATGAG
    AATGATGAAT TTGAGCTAAC GGGAAAAGTA GAGGCAGAGC TCCATCTGTT GACAGCAGAG
    GAGGCAGAGA AGAGTCCAGT CGGCCTGGGG AGGAATGATC CGGAGCCACT TGAGAAACCA
    AATCGTCCAA CTACCAGCCT GTCGTGGCTG TTGAGTCCGA TTCGGGTGAC CAGCATAATG
    TGTTGGCGGC AGTATAAGTT GGTCACAGTG GTGACTCTGC TCATCATAAC CGGGCTGATC
    TTTTTGGGCC TCCTGTTCTT TTCTCTGCCT TCAGCTTTCA TGCAGAAGCT CTTCAACATC
    TCTGGATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009293014.1
    CDS303..6371
    Translation

    Target ORF information:

    RefSeq Version XM_009294739.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio otoferlin a (otofa), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009294739.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGGCTTTAG TGGTGCATCT GAAGACTGTC ACGGAGCTCC GTGGGAAGGG GGACCGAATC 
    GCTAAAGTCA CTTTCAGAGG TCTCTCCTTC TTCTCTCGGG TGTTGGAAAA CTGCGAGGAT
    GAAGCTCGAT TTGAGCAGGC GTTCCGATGG CCAATTGGGA GTCAAGTGGA TGGCGACGAG
    ATGCTGGAGA TCCAAGTGTT TAATTACAGC AAAGTCTTCA CTAATAGGTT AATCGGCACA
    TTCCGCATGG TTCTTCAGAA GGTGGTGGAG GAGGGACACC TGGAGGTGTC TGACACACTC
    ATTGACGACA ATAACACAGC CATACAGACA ACCATTTCCA TAGAGATCAA ATATCAGACC
    ATGGACGGGA GTGTAAAGGT GTGGAGCGAT GGTGAATTCC TTGATATTCC AGATGACCTT
    GATGGAACCT TCCAGTTTGA GACAGATAGT CTTCTCTCAG GACGCAGCCA GAGTTCTGGA
    ACTTCACCTG GACGATCCAT TCATGGCATT CCCACATTCC GCAAGGCAGG AAAAGGAGTG
    TTCTCAGCCA TGAAGCTGGG CAAGACCCGC ACCTCTAAAG ATGACCACAA AAAAGGAGAT
    GATGCTGCCA TTCTGGATGC AGAAGATCTG GATCGAAAAG CCATGCGTCT AGGCGGCATT
    CTTGACCCTG ATACAATCTC ACTGGCCTCG GTTACTGCAG TGACCACTAA TGTCTCCAAC
    AAAAGATCAA AGCCTGACAT TAAAATGGAG CCGAGTTCAG GGCGTCCGGT TGATTATCAG
    ATCAGTGTTA CAGTAATCGA GGCCCGTCAG CTGGTCGGAT TGAACATGGA CCCTGTGGTT
    TGCGTGGAAA TTGGAGAGGA GAAGAAGTAC ACGTCAATGA AGGAGTCAAC AAACTGCCCT
    TACTACAATG AGTATTTTGT CTTCGACTTC CACGTGCCTC CAGACGTCAT GTTTGACAAA
    ATTATAAAGA TTTCAGTAAT ACATTCTAAA AACCTTCTGC GAAGCGGCAC CCTCGTGGGC
    ACCTTCAAAC TAGACGTGGG GACTGTTTAC ACACAACCTG AACATCAGTT TCATCACAAA
    TGGGCGATGC TGTCAGACCC TGATGACATC ACGACTGGCT GCAAAGGTTA TGTTAAATGT
    GACATCGCAG TCGTGGGAAA AGGAGACAAC ATCAAGACTC CACACAAGGC CAGTGAAGCA
    GACGAGGATG ACATAGAGGG GAATCTTCTC TTGCCGGAGG GTGTTCCATC AGAGAGACAG
    TGGGCTCGGT TTTATGTTAA GATTTATAGA GCTGAGGGTC TGCCTAAAAT GAACACCAGT
    ATCATGGCCA ACGTGAAGAA AGCCTTTATT GGGGAAAACA GGGACCTTGT GGACCCTTAC
    GTCCTGGTGC AATTTGCAGG GCAAAAGGGC AAAACATCTG TTCAGAAGAG CAGCTACGAG
    CCCATCTGGA ACGAGCAAGT CATCTTCACG GAGATGTTCC CACCTTTGTG TAGGCGACTG
    AAAGTTCAGA TCCGTGATTC AGACAAGGTG AACGATGTTG CCATAGGAAC CCACTTCATT
    GATCTACGAA AGGTTTCCAA TGATGGAGAC AAAGGTTTCT TGCCCACTAT GGGTCCGGCT
    TGGGTGAATA TGTATGGGTC GACCCGTAAC TACACCCTGA TGGACGAACA CCAGGACCTG
    AATGAGGGGT TGGGGGAAGG GGTGTCCTTC AGAGCACGTC TGCTCATTAG TATCGCGGTG
    GAGATTCTAG ACACCACCTC TGCTGAGATC ATGAGCTCCA CTGAAGTACA CATGGAGCCG
    GTGTCCAACA TCTCAGAGAG TGCTACAGGA AAGATGGAAG AATTTTTCCT TTTCGGGTCA
    TTCTTGGAGG CCACAATGAT AGACAGAAAA ATAGGAGATA AACCCATCAG CTTTGAAGTC
    ACCATCGGTA ACTATGGTAA CCAGATTGAT GGTGTGAGCA AGCCTGCATT AGCGAAGAAG
    AAGAAGGAGG GTGGAGGAGA GAGTGAAGAA GAGGAGTCTG AGCTTATCCA CAACTCCAGC
    GAGGAAGAGG CAGAGGATGA TGGAGATCTG ACCTCTGTAC CGTCTACACC TCCTATGAAG
    CCTGTTATCA CCGACAGAAA TTACTTCCAC TTGCCTTACT TTGAAAAGAA ACCCTGCATT
    TACATCAAGA GCTGGTGGCA GGACCAGCGG AGACGTCTCT ACAACTCCAA CATAATGGAC
    AAGATAGCTG ATAAACTGGA GGAAGGTCTG AATGATGTTC AAGAGATCAT AAAAACAGAG
    AAGGCATATC CAGAACGAAG ACTAAGAGGT GTATTAGAAG AACTTAGCAC AAGCTGCAGC
    CGGTTTGTTA CACTTGCAAA CAAGGATCAG AATCTTTCTG GAAGAACCAA GCTGGACAGA
    GAGAGGCTGA AGTCCTGTAT GAGAGAAATG GAGAGTATGG GTCAGCAGGC GAAGACCATC
    CGCACACAAG TGAAAAGAAA CACAGTTCGA GATAAACTCA AGCTAGTGTT GAATTTCCTT
    CAGAGGCTTC GATTCTTAGC AGACGAGCCC CAGCACAGCA TCCCTGATGT GTTCATATGG
    ATGATTAGCA ACAACAAACG CATCGCATAC GCCCGCATAC CCTCCAAAGA CATCCTCTAC
    TCAATTGTCG ATGAAGAGAT GGGCAAAGAC TGTGGAAAAG TTAAAGCTGT TTTTCTCAGG
    CTGCCTGGAA AGAAAGGTTT TGGGCCTGCT GGCTGGACAG TTCAGGCTAA ATTAGAGATG
    TATTTGTGGC TGGGCCTGAA TAAACAGAGG AAAGACTTCC TGATTGGCCT CCCTAGTGGC
    TTTGAAGAAA ACAAGGCAGT AAAGGGAATT GGCATACAAG CTGTCCCTCC CATCAGCCTA
    GTTTATAACA TGAAGCAGGT GTTTCAGCTG AGGGCCCACA TGTATCAAGC TCGCAGTCTG
    TTTGCTGCGG ACACTAGTGG CCTGTCTGAT CCATTTGCAA GGGTTTTCTT CTCCACCCAC
    AGCCAGGTCA CGGAGGTCCT CAGTGAGACT CTCTGTCCAA CATGGGATCA GCTGCTTGTG
    TTTGATAATG TTGAACTTTA CGGTGAGGCC GGTGAACTCC GTGATGACCC TCCAATCATT
    GTCATTGAGT TGTATGATCA AGACACTGTG GGGAAAGCTG AGTTTATTGG CCGAACATTT
    GCAAAACCAC TTACTAAGAT GGTTGATGAA CACTACGGCC CACCGCGGTT TCCTCCCCAG
    CTCGAGTATT ATCAGATCTA CCGAGGAAAC TGTGCTGCGG GAGATCTATT GGCTGCTTTT
    GAGCTTCTAC AGATTGGTCC AGCAGGGCGG GCAGCACTTC CTCCAATCGA TGGACCGACC
    GATTCTGACC GTGGGCCCAT TCTGCCTGTT CCATTGGGAA TTCGACCAGT TCTCAGCAGA
    TATCGCATCG AGGTCCTGTT TTGGGGTCTG AGGGACCTTA AAAGGGTCAA CCTGGCTCAG
    GTGGACCGAC CTCGTGTGGA CATTGAGTGT GCAGGGAAAG GAGTTCAGTC AGCCCTAATT
    CAAAACTACA AGAAGAATCC AAACTTCAGC ACATTAGTAA AGTGGTTTGA AGTGGATCTG
    CCAGAAAATG AGCTGCTCCA TCCACCACTT AATATTCGGG TAGTGGATTG CAGGGCATTC
    GGACGCTACA TTTTGGTGGG GTCTCATGCT GTGACCACCC TTCGCAAGTT CATCTACAGC
    CCCCCAGACA AGACTGCTAA CAACTGGGCT CACACAGCTA GACTGGCCAA TGGCTACCTG
    GCTCTCAGCA ATGGGACGTC TCATTCCCGC CCCCAATCCC GTCCTGTTTC TTATCCCTCA
    GGTGACATTG TGGTCAACAT GAGCCCTGAA CCTAACATTA AGAAGATGGA CACGGTTGTC
    AAGATTGAAG CTACCACCGA TGCGGTTGTT AAAGTTGACT TGAATGAGGA TGAGAAGGAG
    AAAGAAAAGA AGAAGAAGAA GAAGAAGAAA GGAGAAGAAG TGGAGGAAGA GGAGCCGGAT
    GAGAGCATGC TGGACTGGTG GTCCAAATAC TTTGCCTCAA TAGAGACTAT GATGGAGAAT
    CTAAGAGCTC AGGAGGCCGC TCAGGCAGAG GCAGAGGAAA GAGAAGATTT GGAGATTGCA
    GCAGAGAGTG CAGAGATCAA AGCTGATGAC TTTCCTATGA AAGGCACCAA ACCTAAAGAG
    AAGAGCAAAG ACAAGAAGAG CACCAAGGAC AAAAAGAAGA ACAACGATGG CACAGAAAAA
    CGACCTCCAA AACCAAAAGT TGATGAACTC ATGGTGTACA ATAAAGAGCT GGAGAGTGAG
    TTCGGTAGCT TTGAGGACTG GCTTCACACC TTCAACCTGT ACCGAGGAAA GGCTGGAGAT
    GATGATGACC ACAATGTGGT TGATGAGGAC AGAATTGTGG GCAGATTCAA GGGCTCCCTC
    TGTATGTATA AACTACCACT GTCTGAGGAG ATCACCAGAG AGGCAGGATT TGATCCAAAC
    ATGGGCATGT TCCAGAGTAT TCCTCACAAT GACCCTATCA ACGTTCTTGT CAGGATTTAT
    ATCATTCGAG CCACAGACCT GCATCCTGCA GATATAAATG GAAAGGCCGA CCCTTATATA
    GTCATTAAAT TGGGAAAGTC AGACATTCGA GACAAGGAGA ACTACATATC CAAGCAGCTG
    AATCCTGTCT TTGGAAAGTC ATTTGATATA GAGGCCACGT TCCCAATGGA GTCTATGCTG
    ACTGTGGCAG TGTATGACTG GGATTTAGTC GGAACTGATG ACCTGATTGG TGAGACTAAG
    ATTGATTTGG AGAACAGATA CTACAGCAAA CACAGAGCCA CATGTGGCAT TGCTTCCAAC
    TACTCTGTCC ATGGTTATAA TGTATGGAGA GATCCTCAGA AACCAGCTCA GATCCTGGCT
    AAGTTGTGCA AAGAAGGTAA ACTGGATGGA CCTCACTATG GACCTGGGGG AAGAGTCAAA
    GTTGCAAACC GGATCTTTCT TGGGCCGACA GAAATTGAGG ATGAGAGTGG TCTGAAAAAA
    CAAACAGAAG AACACTTGGC GCTCACAGTC CTTAGACATT GGGAGGAAAT CCCACGTGTT
    GGTTGCAAAC TCATCCCTGA GCATGTGGAG ACGAGGCCTC TCCTCAACCC AGACAAGCCA
    GGCATAGAGC AGGGAAGGAT TGAAATGTGG GTGGACATGT TTCCTATGGA TGTCCCAGCA
    CCAGGACCAG CCATTGATAT ATCGCCACGC AAACCAAAGA GATATGAGCT CAGGGTGATA
    ATATGGAATA CTGACGAGGT AATTCTGGAA GACGATGATT ACTTCACAGG GGAAAAGTCC
    AGTGACATTT TTGTGAGGGG CTGGCTTAAA GGACAGCAGG AGGACAAACA GGACACCGAT
    GTACATTATC ACTCTCTAAC TGGAGAGGGA AACTTCAACT GGCGCTTTGT CTTCCCCTTT
    GACTATCTCA TGGCCGAGGA GAAGATTGTT ATCTCCAAGA AGGAATCAAT GTTTTCCTGG
    GATGAGACTG AGTATAAGAT CCCTGCTCGA CTCACCCTTC AAGTATGGGA TGCCGATCAC
    TTTTCTGCAG ATGATTTTCT GGGTGCGATT GAGTTGGACT TGAATAAGTT TCCGCGTGGG
    GCAAAAACAG CCAAGCAGTG CTCTCTAGAC ATGGTTCTCA AAGAGCATGA GCTGCCAACC
    ATCTCCATCT TCAAACAAAA GAGAGTCAAG GGCTGGTGGC CATTTGTGGC CCAAAATGAG
    AATGATGAAT TTGAGCTAAC GGGAAAAGTA GAGGCAGAGC TCCATCTGTT GACAGCAGAG
    GAGGCAGAGA AGAGTCCAGT CGGCCTGGGG AGGAATGATC CGGAGCCACT TGAGAAACCA
    AATCGTCCAA CTACCAGCCT GTCGTGGCTG TTGAGTCCGA TTCGGGTGAC CAGCATAATG
    TGTTGGCGGC AGTATAAGTT GGTCACAGTG GTGACTCTGC TCATCATAAC CGGGCTGATC
    TTTTTGGGCC TCCTGTTCTT TTCTCTGCCT TCAGCTTTCA TGCAGAAGCT CTTCAACATC
    TCTGGATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42610
    Clone ID Related Accession (Same CDS sequence) XM_009294739.2 , XM_009294739.3
    Accession Version XM_009294739.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6069bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product otoferlin isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009294739.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGGCTTTAG TGGTGCATCT GAAGACTGTC ACGGAGCTCC GTGGGAAGGG GGACCGAATC 
    GCTAAAGTCA CTTTCAGAGG TCTCTCCTTC TTCTCTCGGG TGTTGGAAAA CTGCGAGGAT
    GAAGCTCGAT TTGAGCAGGC GTTCCGATGG CCAATTGGGA GTCAAGTGGA TGGCGACGAG
    ATGCTGGAGA TCCAAGTGTT TAATTACAGC AAAGTCTTCA CTAATAGGTT AATCGGCACA
    TTCCGCATGG TTCTTCAGAA GGTGGTGGAG GAGGGACACC TGGAGGTGTC TGACACACTC
    ATTGACGACA ATAACACAGC CATACAGACA ACCATTTCCA TAGAGATCAA ATATCAGACC
    ATGGACGGGA GTGTAAAGGT GTGGAGCGAT GGTGAATTCC TTGATATTCC AGATGACCTT
    GATGGAACCT TCCAGTTTGA GACAGATAGT CTTCTCTCAG GACGCAGCCA GAGTTCTGGA
    ACTTCACCTG GACGATCCAT TCATGGCATT CCCACATTCC GCAAGGCAGG AAAAGGAGTG
    TTCTCAGCCA TGAAGCTGGG CAAGACCCGC ACCTCTAAAG ATGACCACAA AAAAGGAGAT
    GATGCTGCCA TTCTGGATGC AGAAGATCTG GATCGAAAAG CCATGCGTCT AGGCGGCATT
    CTTGACCCTG ATACAATCTC ACTGGCCTCG GTTACTGCAG TGACCACTAA TGTCTCCAAC
    AAAAGATCAA AGCCTGACAT TAAAATGGAG CCGAGTTCAG GGCGTCCGGT TGATTATCAG
    ATCAGTGTTA CAGTAATCGA GGCCCGTCAG CTGGTCGGAT TGAACATGGA CCCTGTGGTT
    TGCGTGGAAA TTGGAGAGGA GAAGAAGTAC ACGTCAATGA AGGAGTCAAC AAACTGCCCT
    TACTACAATG AGTATTTTGT CTTCGACTTC CACGTGCCTC CAGACGTCAT GTTTGACAAA
    ATTATAAAGA TTTCAGTAAT ACATTCTAAA AACCTTCTGC GAAGCGGCAC CCTCGTGGGC
    ACCTTCAAAC TAGACGTGGG GACTGTTTAC ACACAACCTG AACATCAGTT TCATCACAAA
    TGGGCGATGC TGTCAGACCC TGATGACATC ACGACTGGCT GCAAAGGTTA TGTTAAATGT
    GACATCGCAG TCGTGGGAAA AGGAGACAAC ATCAAGACTC CACACAAGGC CAGTGAAGCA
    GACGAGGATG ACATAGAGGG GAATCTTCTC TTGCCGGAGG GTGTTCCATC AGAGAGACAG
    TGGGCTCGGT TTTATGTTAA GATTTATAGA GCTGAGGGTC TGCCTAAAAT GAACACCAGT
    ATCATGGCCA ACGTGAAGAA AGCCTTTATT GGGGAAAACA GGGACCTTGT GGACCCTTAC
    GTCCTGGTGC AATTTGCAGG GCAAAAGGGC AAAACATCTG TTCAGAAGAG CAGCTACGAG
    CCCATCTGGA ACGAGCAAGT CATCTTCACG GAGATGTTCC CACCTTTGTG TAGGCGACTG
    AAAGTTCAGA TCCGTGATTC AGACAAGGTG AACGATGTTG CCATAGGAAC CCACTTCATT
    GATCTACGAA AGGTTTCCAA TGATGGAGAC AAAGGTTTCT TGCCCACTAT GGGTCCGGCT
    TGGGTGAATA TGTATGGGTC GACCCGTAAC TACACCCTGA TGGACGAACA CCAGGACCTG
    AATGAGGGGT TGGGGGAAGG GGTGTCCTTC AGAGCACGTC TGCTCATTAG TATCGCGGTG
    GAGATTCTAG ACACCACCTC TGCTGAGATC ATGAGCTCCA CTGAAGTACA CATGGAGCCG
    GTGTCCAACA TCTCAGAGAG TGCTACAGGA AAGATGGAAG AATTTTTCCT TTTCGGGTCA
    TTCTTGGAGG CCACAATGAT AGACAGAAAA ATAGGAGATA AACCCATCAG CTTTGAAGTC
    ACCATCGGTA ACTATGGTAA CCAGATTGAT GGTGTGAGCA AGCCTGCATT AGCGAAGAAG
    AAGAAGGAGG GTGGAGGAGA GAGTGAAGAA GAGGAGTCTG AGCTTATCCA CAACTCCAGC
    GAGGAAGAGG CAGAGGATGA TGGAGATCTG ACCTCTGTAC CGTCTACACC TCCTATGAAG
    CCTGTTATCA CCGACAGAAA TTACTTCCAC TTGCCTTACT TTGAAAAGAA ACCCTGCATT
    TACATCAAGA GCTGGTGGCA GGACCAGCGG AGACGTCTCT ACAACTCCAA CATAATGGAC
    AAGATAGCTG ATAAACTGGA GGAAGGTCTG AATGATGTTC AAGAGATCAT AAAAACAGAG
    AAGGCATATC CAGAACGAAG ACTAAGAGGT GTATTAGAAG AACTTAGCAC AAGCTGCAGC
    CGGTTTGTTA CACTTGCAAA CAAGGATCAG AATCTTTCTG GAAGAACCAA GCTGGACAGA
    GAGAGGCTGA AGTCCTGTAT GAGAGAAATG GAGAGTATGG GTCAGCAGGC GAAGACCATC
    CGCACACAAG TGAAAAGAAA CACAGTTCGA GATAAACTCA AGCTAGTGTT GAATTTCCTT
    CAGAGGCTTC GATTCTTAGC AGACGAGCCC CAGCACAGCA TCCCTGATGT GTTCATATGG
    ATGATTAGCA ACAACAAACG CATCGCATAC GCCCGCATAC CCTCCAAAGA CATCCTCTAC
    TCAATTGTCG ATGAAGAGAT GGGCAAAGAC TGTGGAAAAG TTAAAGCTGT TTTTCTCAGG
    CTGCCTGGAA AGAAAGGTTT TGGGCCTGCT GGCTGGACAG TTCAGGCTAA ATTAGAGATG
    TATTTGTGGC TGGGCCTGAA TAAACAGAGG AAAGACTTCC TGATTGGCCT CCCTAGTGGC
    TTTGAAGAAA ACAAGGCAGT AAAGGGAATT GGCATACAAG CTGTCCCTCC CATCAGCCTA
    GTTTATAACA TGAAGCAGGT GTTTCAGCTG AGGGCCCACA TGTATCAAGC TCGCAGTCTG
    TTTGCTGCGG ACACTAGTGG CCTGTCTGAT CCATTTGCAA GGGTTTTCTT CTCCACCCAC
    AGCCAGGTCA CGGAGGTCCT CAGTGAGACT CTCTGTCCAA CATGGGATCA GCTGCTTGTG
    TTTGATAATG TTGAACTTTA CGGTGAGGCC GGTGAACTCC GTGATGACCC TCCAATCATT
    GTCATTGAGT TGTATGATCA AGACACTGTG GGGAAAGCTG AGTTTATTGG CCGAACATTT
    GCAAAACCAC TTACTAAGAT GGTTGATGAA CACTACGGCC CACCGCGGTT TCCTCCCCAG
    CTCGAGTATT ATCAGATCTA CCGAGGAAAC TGTGCTGCGG GAGATCTATT GGCTGCTTTT
    GAGCTTCTAC AGATTGGTCC AGCAGGGCGG GCAGCACTTC CTCCAATCGA TGGACCGACC
    GATTCTGACC GTGGGCCCAT TCTGCCTGTT CCATTGGGAA TTCGACCAGT TCTCAGCAGA
    TATCGCATCG AGGTCCTGTT TTGGGGTCTG AGGGACCTTA AAAGGGTCAA CCTGGCTCAG
    GTGGACCGAC CTCGTGTGGA CATTGAGTGT GCAGGGAAAG GAGTTCAGTC AGCCCTAATT
    CAAAACTACA AGAAGAATCC AAACTTCAGC ACATTAGTAA AGTGGTTTGA AGTGGATCTG
    CCAGAAAATG AGCTGCTCCA TCCACCACTT AATATTCGGG TAGTGGATTG CAGGGCATTC
    GGACGCTACA TTTTGGTGGG GTCTCATGCT GTGACCACCC TTCGCAAGTT CATCTACAGC
    CCCCCAGACA AGACTGCTAA CAACTGGGCT CACACAGCTA GACTGGCCAA TGGCTACCTG
    GCTCTCAGCA ATGGGACGTC TCATTCCCGC CCCCAATCCC GTCCTGTTTC TTATCCCTCA
    GGTGACATTG TGGTCAACAT GAGCCCTGAA CCTAACATTA AGAAGATGGA CACGGTTGTC
    AAGATTGAAG CTACCACCGA TGCGGTTGTT AAAGTTGACT TGAATGAGGA TGAGAAGGAG
    AAAGAAAAGA AGAAGAAGAA GAAGAAGAAA GGAGAAGAAG TGGAGGAAGA GGAGCCGGAT
    GAGAGCATGC TGGACTGGTG GTCCAAATAC TTTGCCTCAA TAGAGACTAT GATGGAGAAT
    CTAAGAGCTC AGGAGGCCGC TCAGGCAGAG GCAGAGGAAA GAGAAGATTT GGAGATTGCA
    GCAGAGAGTG CAGAGATCAA AGCTGATGAC TTTCCTATGA AAGGCACCAA ACCTAAAGAG
    AAGAGCAAAG ACAAGAAGAG CACCAAGGAC AAAAAGAAGA ACAACGATGG CACAGAAAAA
    CGACCTCCAA AACCAAAAGT TGATGAACTC ATGGTGTACA ATAAAGAGCT GGAGAGTGAG
    TTCGGTAGCT TTGAGGACTG GCTTCACACC TTCAACCTGT ACCGAGGAAA GGCTGGAGAT
    GATGATGACC ACAATGTGGT TGATGAGGAC AGAATTGTGG GCAGATTCAA GGGCTCCCTC
    TGTATGTATA AACTACCACT GTCTGAGGAG ATCACCAGAG AGGCAGGATT TGATCCAAAC
    ATGGGCATGT TCCAGAGTAT TCCTCACAAT GACCCTATCA ACGTTCTTGT CAGGATTTAT
    ATCATTCGAG CCACAGACCT GCATCCTGCA GATATAAATG GAAAGGCCGA CCCTTATATA
    GTCATTAAAT TGGGAAAGTC AGACATTCGA GACAAGGAGA ACTACATATC CAAGCAGCTG
    AATCCTGTCT TTGGAAAGTC ATTTGATATA GAGGCCACGT TCCCAATGGA GTCTATGCTG
    ACTGTGGCAG TGTATGACTG GGATTTAGTC GGAACTGATG ACCTGATTGG TGAGACTAAG
    ATTGATTTGG AGAACAGATA CTACAGCAAA CACAGAGCCA CATGTGGCAT TGCTTCCAAC
    TACTCTGTCC ATGGTTATAA TGTATGGAGA GATCCTCAGA AACCAGCTCA GATCCTGGCT
    AAGTTGTGCA AAGAAGGTAA ACTGGATGGA CCTCACTATG GACCTGGGGG AAGAGTCAAA
    GTTGCAAACC GGATCTTTCT TGGGCCGACA GAAATTGAGG ATGAGAGTGG TCTGAAAAAA
    CAAACAGAAG AACACTTGGC GCTCACAGTC CTTAGACATT GGGAGGAAAT CCCACGTGTT
    GGTTGCAAAC TCATCCCTGA GCATGTGGAG ACGAGGCCTC TCCTCAACCC AGACAAGCCA
    GGCATAGAGC AGGGAAGGAT TGAAATGTGG GTGGACATGT TTCCTATGGA TGTCCCAGCA
    CCAGGACCAG CCATTGATAT ATCGCCACGC AAACCAAAGA GATATGAGCT CAGGGTGATA
    ATATGGAATA CTGACGAGGT AATTCTGGAA GACGATGATT ACTTCACAGG GGAAAAGTCC
    AGTGACATTT TTGTGAGGGG CTGGCTTAAA GGACAGCAGG AGGACAAACA GGACACCGAT
    GTACATTATC ACTCTCTAAC TGGAGAGGGA AACTTCAACT GGCGCTTTGT CTTCCCCTTT
    GACTATCTCA TGGCCGAGGA GAAGATTGTT ATCTCCAAGA AGGAATCAAT GTTTTCCTGG
    GATGAGACTG AGTATAAGAT CCCTGCTCGA CTCACCCTTC AAGTATGGGA TGCCGATCAC
    TTTTCTGCAG ATGATTTTCT GGGTGCGATT GAGTTGGACT TGAATAAGTT TCCGCGTGGG
    GCAAAAACAG CCAAGCAGTG CTCTCTAGAC ATGGTTCTCA AAGAGCATGA GCTGCCAACC
    ATCTCCATCT TCAAACAAAA GAGAGTCAAG GGCTGGTGGC CATTTGTGGC CCAAAATGAG
    AATGATGAAT TTGAGCTAAC GGGAAAAGTA GAGGCAGAGC TCCATCTGTT GACAGCAGAG
    GAGGCAGAGA AGAGTCCAGT CGGCCTGGGG AGGAATGATC CGGAGCCACT TGAGAAACCA
    AATCGTCCAA CTACCAGCCT GTCGTGGCTG TTGAGTCCGA TTCGGGTGAC CAGCATAATG
    TGTTGGCGGC AGTATAAGTT GGTCACAGTG GTGACTCTGC TCATCATAAC CGGGCTGATC
    TTTTTGGGCC TCCTGTTCTT TTCTCTGCCT TCAGCTTTCA TGCAGAAGCT CTTCAACATC
    TCTGGATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009293014.1
    CDS300..6368
    Translation

    Target ORF information:

    RefSeq Version XM_009294739.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio otoferlin a (otofa), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009294739.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGGCTTTAG TGGTGCATCT GAAGACTGTC ACGGAGCTCC GTGGGAAGGG GGACCGAATC 
    GCTAAAGTCA CTTTCAGAGG TCTCTCCTTC TTCTCTCGGG TGTTGGAAAA CTGCGAGGAT
    GAAGCTCGAT TTGAGCAGGC GTTCCGATGG CCAATTGGGA GTCAAGTGGA TGGCGACGAG
    ATGCTGGAGA TCCAAGTGTT TAATTACAGC AAAGTCTTCA CTAATAGGTT AATCGGCACA
    TTCCGCATGG TTCTTCAGAA GGTGGTGGAG GAGGGACACC TGGAGGTGTC TGACACACTC
    ATTGACGACA ATAACACAGC CATACAGACA ACCATTTCCA TAGAGATCAA ATATCAGACC
    ATGGACGGGA GTGTAAAGGT GTGGAGCGAT GGTGAATTCC TTGATATTCC AGATGACCTT
    GATGGAACCT TCCAGTTTGA GACAGATAGT CTTCTCTCAG GACGCAGCCA GAGTTCTGGA
    ACTTCACCTG GACGATCCAT TCATGGCATT CCCACATTCC GCAAGGCAGG AAAAGGAGTG
    TTCTCAGCCA TGAAGCTGGG CAAGACCCGC ACCTCTAAAG ATGACCACAA AAAAGGAGAT
    GATGCTGCCA TTCTGGATGC AGAAGATCTG GATCGAAAAG CCATGCGTCT AGGCGGCATT
    CTTGACCCTG ATACAATCTC ACTGGCCTCG GTTACTGCAG TGACCACTAA TGTCTCCAAC
    AAAAGATCAA AGCCTGACAT TAAAATGGAG CCGAGTTCAG GGCGTCCGGT TGATTATCAG
    ATCAGTGTTA CAGTAATCGA GGCCCGTCAG CTGGTCGGAT TGAACATGGA CCCTGTGGTT
    TGCGTGGAAA TTGGAGAGGA GAAGAAGTAC ACGTCAATGA AGGAGTCAAC AAACTGCCCT
    TACTACAATG AGTATTTTGT CTTCGACTTC CACGTGCCTC CAGACGTCAT GTTTGACAAA
    ATTATAAAGA TTTCAGTAAT ACATTCTAAA AACCTTCTGC GAAGCGGCAC CCTCGTGGGC
    ACCTTCAAAC TAGACGTGGG GACTGTTTAC ACACAACCTG AACATCAGTT TCATCACAAA
    TGGGCGATGC TGTCAGACCC TGATGACATC ACGACTGGCT GCAAAGGTTA TGTTAAATGT
    GACATCGCAG TCGTGGGAAA AGGAGACAAC ATCAAGACTC CACACAAGGC CAGTGAAGCA
    GACGAGGATG ACATAGAGGG GAATCTTCTC TTGCCGGAGG GTGTTCCATC AGAGAGACAG
    TGGGCTCGGT TTTATGTTAA GATTTATAGA GCTGAGGGTC TGCCTAAAAT GAACACCAGT
    ATCATGGCCA ACGTGAAGAA AGCCTTTATT GGGGAAAACA GGGACCTTGT GGACCCTTAC
    GTCCTGGTGC AATTTGCAGG GCAAAAGGGC AAAACATCTG TTCAGAAGAG CAGCTACGAG
    CCCATCTGGA ACGAGCAAGT CATCTTCACG GAGATGTTCC CACCTTTGTG TAGGCGACTG
    AAAGTTCAGA TCCGTGATTC AGACAAGGTG AACGATGTTG CCATAGGAAC CCACTTCATT
    GATCTACGAA AGGTTTCCAA TGATGGAGAC AAAGGTTTCT TGCCCACTAT GGGTCCGGCT
    TGGGTGAATA TGTATGGGTC GACCCGTAAC TACACCCTGA TGGACGAACA CCAGGACCTG
    AATGAGGGGT TGGGGGAAGG GGTGTCCTTC AGAGCACGTC TGCTCATTAG TATCGCGGTG
    GAGATTCTAG ACACCACCTC TGCTGAGATC ATGAGCTCCA CTGAAGTACA CATGGAGCCG
    GTGTCCAACA TCTCAGAGAG TGCTACAGGA AAGATGGAAG AATTTTTCCT TTTCGGGTCA
    TTCTTGGAGG CCACAATGAT AGACAGAAAA ATAGGAGATA AACCCATCAG CTTTGAAGTC
    ACCATCGGTA ACTATGGTAA CCAGATTGAT GGTGTGAGCA AGCCTGCATT AGCGAAGAAG
    AAGAAGGAGG GTGGAGGAGA GAGTGAAGAA GAGGAGTCTG AGCTTATCCA CAACTCCAGC
    GAGGAAGAGG CAGAGGATGA TGGAGATCTG ACCTCTGTAC CGTCTACACC TCCTATGAAG
    CCTGTTATCA CCGACAGAAA TTACTTCCAC TTGCCTTACT TTGAAAAGAA ACCCTGCATT
    TACATCAAGA GCTGGTGGCA GGACCAGCGG AGACGTCTCT ACAACTCCAA CATAATGGAC
    AAGATAGCTG ATAAACTGGA GGAAGGTCTG AATGATGTTC AAGAGATCAT AAAAACAGAG
    AAGGCATATC CAGAACGAAG ACTAAGAGGT GTATTAGAAG AACTTAGCAC AAGCTGCAGC
    CGGTTTGTTA CACTTGCAAA CAAGGATCAG AATCTTTCTG GAAGAACCAA GCTGGACAGA
    GAGAGGCTGA AGTCCTGTAT GAGAGAAATG GAGAGTATGG GTCAGCAGGC GAAGACCATC
    CGCACACAAG TGAAAAGAAA CACAGTTCGA GATAAACTCA AGCTAGTGTT GAATTTCCTT
    CAGAGGCTTC GATTCTTAGC AGACGAGCCC CAGCACAGCA TCCCTGATGT GTTCATATGG
    ATGATTAGCA ACAACAAACG CATCGCATAC GCCCGCATAC CCTCCAAAGA CATCCTCTAC
    TCAATTGTCG ATGAAGAGAT GGGCAAAGAC TGTGGAAAAG TTAAAGCTGT TTTTCTCAGG
    CTGCCTGGAA AGAAAGGTTT TGGGCCTGCT GGCTGGACAG TTCAGGCTAA ATTAGAGATG
    TATTTGTGGC TGGGCCTGAA TAAACAGAGG AAAGACTTCC TGATTGGCCT CCCTAGTGGC
    TTTGAAGAAA ACAAGGCAGT AAAGGGAATT GGCATACAAG CTGTCCCTCC CATCAGCCTA
    GTTTATAACA TGAAGCAGGT GTTTCAGCTG AGGGCCCACA TGTATCAAGC TCGCAGTCTG
    TTTGCTGCGG ACACTAGTGG CCTGTCTGAT CCATTTGCAA GGGTTTTCTT CTCCACCCAC
    AGCCAGGTCA CGGAGGTCCT CAGTGAGACT CTCTGTCCAA CATGGGATCA GCTGCTTGTG
    TTTGATAATG TTGAACTTTA CGGTGAGGCC GGTGAACTCC GTGATGACCC TCCAATCATT
    GTCATTGAGT TGTATGATCA AGACACTGTG GGGAAAGCTG AGTTTATTGG CCGAACATTT
    GCAAAACCAC TTACTAAGAT GGTTGATGAA CACTACGGCC CACCGCGGTT TCCTCCCCAG
    CTCGAGTATT ATCAGATCTA CCGAGGAAAC TGTGCTGCGG GAGATCTATT GGCTGCTTTT
    GAGCTTCTAC AGATTGGTCC AGCAGGGCGG GCAGCACTTC CTCCAATCGA TGGACCGACC
    GATTCTGACC GTGGGCCCAT TCTGCCTGTT CCATTGGGAA TTCGACCAGT TCTCAGCAGA
    TATCGCATCG AGGTCCTGTT TTGGGGTCTG AGGGACCTTA AAAGGGTCAA CCTGGCTCAG
    GTGGACCGAC CTCGTGTGGA CATTGAGTGT GCAGGGAAAG GAGTTCAGTC AGCCCTAATT
    CAAAACTACA AGAAGAATCC AAACTTCAGC ACATTAGTAA AGTGGTTTGA AGTGGATCTG
    CCAGAAAATG AGCTGCTCCA TCCACCACTT AATATTCGGG TAGTGGATTG CAGGGCATTC
    GGACGCTACA TTTTGGTGGG GTCTCATGCT GTGACCACCC TTCGCAAGTT CATCTACAGC
    CCCCCAGACA AGACTGCTAA CAACTGGGCT CACACAGCTA GACTGGCCAA TGGCTACCTG
    GCTCTCAGCA ATGGGACGTC TCATTCCCGC CCCCAATCCC GTCCTGTTTC TTATCCCTCA
    GGTGACATTG TGGTCAACAT GAGCCCTGAA CCTAACATTA AGAAGATGGA CACGGTTGTC
    AAGATTGAAG CTACCACCGA TGCGGTTGTT AAAGTTGACT TGAATGAGGA TGAGAAGGAG
    AAAGAAAAGA AGAAGAAGAA GAAGAAGAAA GGAGAAGAAG TGGAGGAAGA GGAGCCGGAT
    GAGAGCATGC TGGACTGGTG GTCCAAATAC TTTGCCTCAA TAGAGACTAT GATGGAGAAT
    CTAAGAGCTC AGGAGGCCGC TCAGGCAGAG GCAGAGGAAA GAGAAGATTT GGAGATTGCA
    GCAGAGAGTG CAGAGATCAA AGCTGATGAC TTTCCTATGA AAGGCACCAA ACCTAAAGAG
    AAGAGCAAAG ACAAGAAGAG CACCAAGGAC AAAAAGAAGA ACAACGATGG CACAGAAAAA
    CGACCTCCAA AACCAAAAGT TGATGAACTC ATGGTGTACA ATAAAGAGCT GGAGAGTGAG
    TTCGGTAGCT TTGAGGACTG GCTTCACACC TTCAACCTGT ACCGAGGAAA GGCTGGAGAT
    GATGATGACC ACAATGTGGT TGATGAGGAC AGAATTGTGG GCAGATTCAA GGGCTCCCTC
    TGTATGTATA AACTACCACT GTCTGAGGAG ATCACCAGAG AGGCAGGATT TGATCCAAAC
    ATGGGCATGT TCCAGAGTAT TCCTCACAAT GACCCTATCA ACGTTCTTGT CAGGATTTAT
    ATCATTCGAG CCACAGACCT GCATCCTGCA GATATAAATG GAAAGGCCGA CCCTTATATA
    GTCATTAAAT TGGGAAAGTC AGACATTCGA GACAAGGAGA ACTACATATC CAAGCAGCTG
    AATCCTGTCT TTGGAAAGTC ATTTGATATA GAGGCCACGT TCCCAATGGA GTCTATGCTG
    ACTGTGGCAG TGTATGACTG GGATTTAGTC GGAACTGATG ACCTGATTGG TGAGACTAAG
    ATTGATTTGG AGAACAGATA CTACAGCAAA CACAGAGCCA CATGTGGCAT TGCTTCCAAC
    TACTCTGTCC ATGGTTATAA TGTATGGAGA GATCCTCAGA AACCAGCTCA GATCCTGGCT
    AAGTTGTGCA AAGAAGGTAA ACTGGATGGA CCTCACTATG GACCTGGGGG AAGAGTCAAA
    GTTGCAAACC GGATCTTTCT TGGGCCGACA GAAATTGAGG ATGAGAGTGG TCTGAAAAAA
    CAAACAGAAG AACACTTGGC GCTCACAGTC CTTAGACATT GGGAGGAAAT CCCACGTGTT
    GGTTGCAAAC TCATCCCTGA GCATGTGGAG ACGAGGCCTC TCCTCAACCC AGACAAGCCA
    GGCATAGAGC AGGGAAGGAT TGAAATGTGG GTGGACATGT TTCCTATGGA TGTCCCAGCA
    CCAGGACCAG CCATTGATAT ATCGCCACGC AAACCAAAGA GATATGAGCT CAGGGTGATA
    ATATGGAATA CTGACGAGGT AATTCTGGAA GACGATGATT ACTTCACAGG GGAAAAGTCC
    AGTGACATTT TTGTGAGGGG CTGGCTTAAA GGACAGCAGG AGGACAAACA GGACACCGAT
    GTACATTATC ACTCTCTAAC TGGAGAGGGA AACTTCAACT GGCGCTTTGT CTTCCCCTTT
    GACTATCTCA TGGCCGAGGA GAAGATTGTT ATCTCCAAGA AGGAATCAAT GTTTTCCTGG
    GATGAGACTG AGTATAAGAT CCCTGCTCGA CTCACCCTTC AAGTATGGGA TGCCGATCAC
    TTTTCTGCAG ATGATTTTCT GGGTGCGATT GAGTTGGACT TGAATAAGTT TCCGCGTGGG
    GCAAAAACAG CCAAGCAGTG CTCTCTAGAC ATGGTTCTCA AAGAGCATGA GCTGCCAACC
    ATCTCCATCT TCAAACAAAA GAGAGTCAAG GGCTGGTGGC CATTTGTGGC CCAAAATGAG
    AATGATGAAT TTGAGCTAAC GGGAAAAGTA GAGGCAGAGC TCCATCTGTT GACAGCAGAG
    GAGGCAGAGA AGAGTCCAGT CGGCCTGGGG AGGAATGATC CGGAGCCACT TGAGAAACCA
    AATCGTCCAA CTACCAGCCT GTCGTGGCTG TTGAGTCCGA TTCGGGTGAC CAGCATAATG
    TGTTGGCGGC AGTATAAGTT GGTCACAGTG GTGACTCTGC TCATCATAAC CGGGCTGATC
    TTTTTGGGCC TCCTGTTCTT TTCTCTGCCT TCAGCTTTCA TGCAGAAGCT CTTCAACATC
    TCTGGATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42611
    Clone ID Related Accession (Same CDS sequence) XM_009294740.2 , XM_009294740.3
    Accession Version XM_009294740.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6057bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product otoferlin isoform X7
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009294740.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGGCTTTAG TGGTGCATCT GAAGACTGTC ACGGAGCTCC GTGGGAAGGG GGACCGAATC 
    GCTAAAGTCA CTTTCAGAGG TCTCTCCTTC TTCTCTCGGG TGTTGGAAAA CTGCGAGGAT
    GAAGCTCGAT TTGAGCAGGC GTTCCGATGG CCAATTGGGA GTCAAGTGGA TGGCGACGAG
    ATGCTGGAGA TCCAAGTGTT TAATTACAGC AAAGTCTTCA CTAATAGGTT AATCGGCACA
    TTCCGCATGG TTCTTCAGAA GGTGGTGGAG GAGGGACACC TGGAGGTGTC TGACACACTC
    ATTGACGACA ATAACACAGC CATACAGACA ACCATTTCCA TAGAGATCAA ATATCAGACC
    ATGGACGGGA GTGTAAAGGT GTGGAGCGAT GGTGAATTCC TTGATATTCC AGATGACCTT
    GATGGAACCT TCCAGTTTGA GACAGATAGT CTTCTCTCAG GACGCAGCCA GAGTTCTGGA
    ACTTCACCTG GACGATCCAT TCATGGCATT CCCACATTCC GCAAGGCAGG AAAAGGAGTG
    TTCTCAGCCA TGAAGCTGGG CAAGACCCGC ACCTCTAAAG ATGACCACAA AAAAGGAGAT
    GATGCTGCCA TTCTGGATGC AGAAGATCTG GATCGAAAAG CCATGCGTCT AGGCGGCATT
    CTTGACCCTG ATACAATCTC ACTGGCCTCG GTTACTGCAG TGACCACTAA TGTCTCCAAC
    AAAAGATCAA AGCCTGACAT TAAAATGGAG CCGAGTTCAG GGCGTCCGGT TGATTATCAG
    ATCAGTGTTA CAGTAATCGA GGCCCGTCAG CTGGTCGGAT TGAACATGGA CCCTGTGGTT
    TGCGTGGAAA TTGGAGAGGA GAAGAAGTAC ACGTCAATGA AGGAGTCAAC AAACTGCCCT
    TACTACAATG AGTATTTTGT CTTCGACTTC CACGTGCCTC CAGACGTCAT GTTTGACAAA
    ATTATAAAGA TTTCAGTAAT ACATTCTAAA AACCTTCTGC GAAGCGGCAC CCTCGTGGGC
    ACCTTCAAAC TAGACGTGGG GACTGTTTAC ACACAACCTG AACATCAGTT TCATCACAAA
    TGGGCGATGC TGTCAGACCC TGATGACATC ACGACTGGCT GCAAAGGTTA TGTTAAATGT
    GACATCGCAG TCGTGGGAAA AGGAGACAAC ATCAAGACTC CACACAAGGC CAGTGAAGCA
    GACGAGGATG ACATAGAGGG GAATCTTCTC TTGCCGGAGG GTGTTCCATC AGAGAGACAG
    TGGGCTCGGT TTTATGTTAA GATTTATAGA GCTGAGGGTC TGCCTAAAAT GAACACCAGT
    ATCATGGCCA ACGTGAAGAA AGCCTTTATT GGGGAAAACA GGGACCTTGT GGACCCTTAC
    GTCCTGGTGC AATTTGCAGG GCAAAAGGGC AAAACATCTG TTCAGAAGAG CAGCTACGAG
    CCCATCTGGA ACGAGCAAGT CATCTTCACG GAGATGTTCC CACCTTTGTG TAGGCGACTG
    AAAGTTCAGA TCCGTGATTC AGACAAGGTG AACGATGTTG CCATAGGAAC CCACTTCATT
    GATCTACGAA AGGTTTCCAA TGATGGAGAC AAAGGTTTCT TGCCCACTAT GGGTCCGGCT
    TGGGTGAATA TGTATGGGTC GACCCGTAAC TACACCCTGA TGGACGAACA CCAGGACCTG
    AATGAGGGGT TGGGGGAAGG GGTGTCCTTC AGAGCACGTC TGCTCATTAG TATCGCGGTG
    GAGATTCTAG ACACCACCTC TGCTGAGATC ATGAGCTCCA CTGAAGTACA CATGGAGCCG
    GTGTCCAACA TCTCAGAGAG TGCTACAGGA AAGATGGAAG AATTTTTCCT TTTCGGGTCA
    TTCTTGGAGG CCACAATGAT AGACAGAAAA ATAGGAGATA AACCCATCAG CTTTGAAGTC
    ACCATCGGTA ACTATGGTAA CCAGATTGAT GGTGTGAGCA AGCCTGCATT AGCGAAGAAG
    AAGAAGGAGG GTGGAGGAGA GAGTGAAGAA GAGGAGTCTG AGCTTATCCA CAACTCCAGC
    GAGGAAGAGG CAGAGGATGA TGGAGATCTG ACCTCTGTAC CGTCTACACC TCCTATGAAG
    CCTGTTATCA CCGACAGAAA TTACTTCCAC TTGCCTTACT TTGAAAAGAA ACCCTGCATT
    TACATCAAGA GCTGGTGGCA GGACCAGCGG AGACGTCTCT ACAACTCCAA CATAATGGAC
    AAGATAGCTG ATAAACTGGA GGAAGGTCTG AATGATGTTC AAGAGATCAT AAAAACAGAG
    AAGGCATATC CAGAACGAAG ACTAAGAGGT GTATTAGAAG AACTTAGCAC AAGCTGCAGC
    CGGTTTGTTA CACTTGCAAA CAAGGATCAG AATCTTTCTG GAAGAACCAA GCTGGACAGA
    GAGAGGCTGA AGTCCTGTAT GAGAGAAATG GAGAGTATGG GTCAGCAGGC GAAGACCATC
    CGCACACAAG TGAAAAGAAA CACAGTTCGA GATAAACTCA AGCTAGTGTT GAATTTCCTT
    CAGAGGCTTC GATTCTTAGC AGACGAGCCC CAGCACAGCA TCCCTGATGT GTTCATATGG
    ATGATTAGCA ACAACAAACG CATCGCATAC GCCCGCATAC CCTCCAAAGA CATCCTCTAC
    TCAATTGTCG ATGAAGAGAT GGGCAAAGAC TGTGGAAAAG TTAAAGCTGT TTTTCTCAGG
    CTGCCTGGAA AGAAAGGTTT TGGGCCTGCT GGCTGGACAG TTCAGGCTAA ATTAGAGATG
    TATTTGTGGC TGGGCCTGAA TAAACAGAGG AAAGACTTCC TGATTGGCCT CCCTAGTGGC
    TTTGAAGAAA ACAAGGCAGT AAAGGGAATT GGCATACAAG CTGTCCCTCC CATCAGCCTA
    GTTTATAACA TGAAGCAGGT GTTTCAGCTG AGGGCCCACA TGTATCAAGC TCGCAGTCTG
    TTTGCTGCGG ACACTAGTGG CCTGTCTGAT CCATTTGCAA GGGTTTTCTT CTCCACCCAC
    AGCCAGGTCA CGGAGGTCCT CAGTGAGACT CTCTGTCCAA CATGGGATCA GCTGCTTGTG
    TTTGATAATG TTGAACTTTA CGGTGAGGCC GGTGAACTCC GTGATGACCC TCCAATCATT
    GTCATTGAGT TGTATGATCA AGACACTGTG GGGAAAGCTG AGTTTATTGG CCGAACATTT
    GCAAAACCAC TTACTAAGAT GGTTGATGAA CACTACGGCC CACCGCGGTT TCCTCCCCAG
    CTCGAGTATT ATCAGATCTA CCGAGGAAAC TGTGCTGCGG GAGATCTATT GGCTGCTTTT
    GAGCTTCTAC AGATTCCTTA TGACGATGAG GAGATCAGAA GAGCTCTTAT TGCTGTCCAT
    GACTTTGCTG TGCCTCAGAT CAAGATTGGT CCAGCAGGGC GGGCAGCACT TCCTCCAATC
    GATGGACCGA CCGATTCTGA CCGTGGGCCC ATTCTGCCTG TTCCATTGGG AATTCGACCA
    GTTCTCAGCA GATATCGCAT CGAGGTCCTG TTTTGGGGTC TGAGGGACCT TAAAAGGGTC
    AACCTGGCTC AGGTGGACCG ACCTCGTGTG GACATTGAGT GTGCAGGGAA AGGAGTTCAG
    TCAGCCCTAA TTCAAAACTA CAAGAAGAAT CCAAACTTCA GCACATTAGT AAAGTGGTTT
    GAAGTGGATC TGCCAGAAAA TGAGCTGCTC CATCCACCAC TTAATATTCG GGTAGTGGAT
    TGCAGGGCAT TCGGACGCTA CATTTTGGTG GGGTCTCATG CTGTGACCAC CCTTCGCAAG
    TTCATCTACA GCCCCCCAGA CAAGACTGCT AACAACTGGG CTCACACAGG TGACATTGTG
    GTCAACATGA GCCCTGAACC TAACATTAAG AAGATGGACA CGGTTGTCAA GATTGAAGCT
    ACCACCGATG CGGTTGTTAA AGTTGACTTG AATGAGGATG AGAAGGAGAA AGAAAAGAAG
    AAGAAGAAGA AGAAGAAAGG AGAAGAAGTG GAGGAAGAGG AGCCGGATGA GAGCATGCTG
    GACTGGTGGT CCAAATACTT TGCCTCAATA GAGACTATGA TGGAGAATCT AAGAGCTCAG
    GAGGCCGCTC AGGCAGAGGC AGAGGAAAGA GAAGATTTGG AGATTGCAGC AGAGAGTGCA
    GAGATCAAAG CTGATGACTT TCCTATGAAA GGCACCAAAC CTAAAGAGAA GAGCAAAGAC
    AAGAAGAGCA CCAAGGACAA AAAGAAGAAC AACGATGGCA CAGAAAAACG ACCTCCAAAA
    CCAAAAGTTG ATGAACTCAT GGTGTACAAT AAAGAGCTGG AGAGTGAGTT CGGTAGCTTT
    GAGGACTGGC TTCACACCTT CAACCTGTAC CGAGGAAAGG CTGGAGATGA TGATGACCAC
    AATGTGGTTG ATGAGGACAG AATTGTGGGC AGATTCAAGG GCTCCCTCTG TATGTATAAA
    CTACCACTGT CTGAGGAGAT CACCAGAGAG GCAGGATTTG ATCCAAACAT GGGCATGTTC
    CAGAGTATTC CTCACAATGA CCCTATCAAC GTTCTTGTCA GGATTTATAT CATTCGAGCC
    ACAGACCTGC ATCCTGCAGA TATAAATGGA AAGGCCGACC CTTATATAGT CATTAAATTG
    GGAAAGTCAG ACATTCGAGA CAAGGAGAAC TACATATCCA AGCAGCTGAA TCCTGTCTTT
    GGAAAGTCAT TTGATATAGA GGCCACGTTC CCAATGGAGT CTATGCTGAC TGTGGCAGTG
    TATGACTGGG ATTTAGTCGG AACTGATGAC CTGATTGGTG AGACTAAGAT TGATTTGGAG
    AACAGATACT ACAGCAAACA CAGAGCCACA TGTGGCATTG CTTCCAACTA CTCTGTCCAT
    GGTTATAATG TATGGAGAGA TCCTCAGAAA CCAGCTCAGA TCCTGGCTAA GTTGTGCAAA
    GAAGGTAAAC TGGATGGACC TCACTATGGA CCTGGGGGAA GAGTCAAAGT TGCAAACCGG
    ATCTTTCTTG GGCCGACAGA AATTGAGGAT GAGAGTGGTC TGAAAAAACA AACAGAAGAA
    CACTTGGCGC TCACAGTCCT TAGACATTGG GAGGAAATCC CACGTGTTGG TTGCAAACTC
    ATCCCTGAGC ATGTGGAGAC GAGGCCTCTC CTCAACCCAG ACAAGCCAGG CATAGAGCAG
    GGAAGGATTG AAATGTGGGT GGACATGTTT CCTATGGATG TCCCAGCACC AGGACCAGCC
    ATTGATATAT CGCCACGCAA ACCAAAGAGA TATGAGCTCA GGGTGATAAT ATGGAATACT
    GACGAGGTAA TTCTGGAAGA CGATGATTAC TTCACAGGGG AAAAGTCCAG TGACATTTTT
    GTGAGGGGCT GGCTTAAAGG ACAGCAGGAG GACAAACAGG ACACCGATGT ACATTATCAC
    TCTCTAACTG GAGAGGGAAA CTTCAACTGG CGCTTTGTCT TCCCCTTTGA CTATCTCATG
    GCCGAGGAGA AGATTGTTAT CTCCAAGAAG GAATCAATGT TTTCCTGGGA TGAGACTGAG
    TATAAGATCC CTGCTCGACT CACCCTTCAA GTATGGGATG CCGATCACTT TTCTGCAGAT
    GATTTTCTGG GTGCGATTGA GTTGGACTTG AATAAGTTTC CGCGTGGGGC AAAAACAGCC
    AAGCAGTGCT CTCTAGACAT GGTTCTCAAA GAGCATGAGC TGCCAACCAT CTCCATCTTC
    AAACAAAAGA GAGTCAAGGG CTGGTGGCCA TTTGTGGCCC AAAATGAGAA TGATGAATTT
    GAGCTAACGG GAAAAGTAGA GGCAGAGCTC CATCTGTTGA CAGCAGAGGA GGCAGAGAAG
    AGTCCAGTCG GCCTGGGGAG GAATGATCCG GAGCCACTTG AGAAACCAAA TCGTCCAACT
    ACCAGCCTGT CGTGGCTGTT GAGTCCGATT CGGGTGACCA GCATAATGTG TTGGCGGCAG
    TATAAGTTGG TCACAGTGGT GACTCTGCTC ATCATAACCG GGCTGATCTT TTTGGGCCTC
    CTGTTCTTTT CTCTGCCTTC AGCTTTCATG CAGAAGCTCT TCAACATCTC TGGATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009293015.1
    CDS303..6359
    Translation

    Target ORF information:

    RefSeq Version XM_009294740.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio otoferlin a (otofa), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009294740.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGGCTTTAG TGGTGCATCT GAAGACTGTC ACGGAGCTCC GTGGGAAGGG GGACCGAATC 
    GCTAAAGTCA CTTTCAGAGG TCTCTCCTTC TTCTCTCGGG TGTTGGAAAA CTGCGAGGAT
    GAAGCTCGAT TTGAGCAGGC GTTCCGATGG CCAATTGGGA GTCAAGTGGA TGGCGACGAG
    ATGCTGGAGA TCCAAGTGTT TAATTACAGC AAAGTCTTCA CTAATAGGTT AATCGGCACA
    TTCCGCATGG TTCTTCAGAA GGTGGTGGAG GAGGGACACC TGGAGGTGTC TGACACACTC
    ATTGACGACA ATAACACAGC CATACAGACA ACCATTTCCA TAGAGATCAA ATATCAGACC
    ATGGACGGGA GTGTAAAGGT GTGGAGCGAT GGTGAATTCC TTGATATTCC AGATGACCTT
    GATGGAACCT TCCAGTTTGA GACAGATAGT CTTCTCTCAG GACGCAGCCA GAGTTCTGGA
    ACTTCACCTG GACGATCCAT TCATGGCATT CCCACATTCC GCAAGGCAGG AAAAGGAGTG
    TTCTCAGCCA TGAAGCTGGG CAAGACCCGC ACCTCTAAAG ATGACCACAA AAAAGGAGAT
    GATGCTGCCA TTCTGGATGC AGAAGATCTG GATCGAAAAG CCATGCGTCT AGGCGGCATT
    CTTGACCCTG ATACAATCTC ACTGGCCTCG GTTACTGCAG TGACCACTAA TGTCTCCAAC
    AAAAGATCAA AGCCTGACAT TAAAATGGAG CCGAGTTCAG GGCGTCCGGT TGATTATCAG
    ATCAGTGTTA CAGTAATCGA GGCCCGTCAG CTGGTCGGAT TGAACATGGA CCCTGTGGTT
    TGCGTGGAAA TTGGAGAGGA GAAGAAGTAC ACGTCAATGA AGGAGTCAAC AAACTGCCCT
    TACTACAATG AGTATTTTGT CTTCGACTTC CACGTGCCTC CAGACGTCAT GTTTGACAAA
    ATTATAAAGA TTTCAGTAAT ACATTCTAAA AACCTTCTGC GAAGCGGCAC CCTCGTGGGC
    ACCTTCAAAC TAGACGTGGG GACTGTTTAC ACACAACCTG AACATCAGTT TCATCACAAA
    TGGGCGATGC TGTCAGACCC TGATGACATC ACGACTGGCT GCAAAGGTTA TGTTAAATGT
    GACATCGCAG TCGTGGGAAA AGGAGACAAC ATCAAGACTC CACACAAGGC CAGTGAAGCA
    GACGAGGATG ACATAGAGGG GAATCTTCTC TTGCCGGAGG GTGTTCCATC AGAGAGACAG
    TGGGCTCGGT TTTATGTTAA GATTTATAGA GCTGAGGGTC TGCCTAAAAT GAACACCAGT
    ATCATGGCCA ACGTGAAGAA AGCCTTTATT GGGGAAAACA GGGACCTTGT GGACCCTTAC
    GTCCTGGTGC AATTTGCAGG GCAAAAGGGC AAAACATCTG TTCAGAAGAG CAGCTACGAG
    CCCATCTGGA ACGAGCAAGT CATCTTCACG GAGATGTTCC CACCTTTGTG TAGGCGACTG
    AAAGTTCAGA TCCGTGATTC AGACAAGGTG AACGATGTTG CCATAGGAAC CCACTTCATT
    GATCTACGAA AGGTTTCCAA TGATGGAGAC AAAGGTTTCT TGCCCACTAT GGGTCCGGCT
    TGGGTGAATA TGTATGGGTC GACCCGTAAC TACACCCTGA TGGACGAACA CCAGGACCTG
    AATGAGGGGT TGGGGGAAGG GGTGTCCTTC AGAGCACGTC TGCTCATTAG TATCGCGGTG
    GAGATTCTAG ACACCACCTC TGCTGAGATC ATGAGCTCCA CTGAAGTACA CATGGAGCCG
    GTGTCCAACA TCTCAGAGAG TGCTACAGGA AAGATGGAAG AATTTTTCCT TTTCGGGTCA
    TTCTTGGAGG CCACAATGAT AGACAGAAAA ATAGGAGATA AACCCATCAG CTTTGAAGTC
    ACCATCGGTA ACTATGGTAA CCAGATTGAT GGTGTGAGCA AGCCTGCATT AGCGAAGAAG
    AAGAAGGAGG GTGGAGGAGA GAGTGAAGAA GAGGAGTCTG AGCTTATCCA CAACTCCAGC
    GAGGAAGAGG CAGAGGATGA TGGAGATCTG ACCTCTGTAC CGTCTACACC TCCTATGAAG
    CCTGTTATCA CCGACAGAAA TTACTTCCAC TTGCCTTACT TTGAAAAGAA ACCCTGCATT
    TACATCAAGA GCTGGTGGCA GGACCAGCGG AGACGTCTCT ACAACTCCAA CATAATGGAC
    AAGATAGCTG ATAAACTGGA GGAAGGTCTG AATGATGTTC AAGAGATCAT AAAAACAGAG
    AAGGCATATC CAGAACGAAG ACTAAGAGGT GTATTAGAAG AACTTAGCAC AAGCTGCAGC
    CGGTTTGTTA CACTTGCAAA CAAGGATCAG AATCTTTCTG GAAGAACCAA GCTGGACAGA
    GAGAGGCTGA AGTCCTGTAT GAGAGAAATG GAGAGTATGG GTCAGCAGGC GAAGACCATC
    CGCACACAAG TGAAAAGAAA CACAGTTCGA GATAAACTCA AGCTAGTGTT GAATTTCCTT
    CAGAGGCTTC GATTCTTAGC AGACGAGCCC CAGCACAGCA TCCCTGATGT GTTCATATGG
    ATGATTAGCA ACAACAAACG CATCGCATAC GCCCGCATAC CCTCCAAAGA CATCCTCTAC
    TCAATTGTCG ATGAAGAGAT GGGCAAAGAC TGTGGAAAAG TTAAAGCTGT TTTTCTCAGG
    CTGCCTGGAA AGAAAGGTTT TGGGCCTGCT GGCTGGACAG TTCAGGCTAA ATTAGAGATG
    TATTTGTGGC TGGGCCTGAA TAAACAGAGG AAAGACTTCC TGATTGGCCT CCCTAGTGGC
    TTTGAAGAAA ACAAGGCAGT AAAGGGAATT GGCATACAAG CTGTCCCTCC CATCAGCCTA
    GTTTATAACA TGAAGCAGGT GTTTCAGCTG AGGGCCCACA TGTATCAAGC TCGCAGTCTG
    TTTGCTGCGG ACACTAGTGG CCTGTCTGAT CCATTTGCAA GGGTTTTCTT CTCCACCCAC
    AGCCAGGTCA CGGAGGTCCT CAGTGAGACT CTCTGTCCAA CATGGGATCA GCTGCTTGTG
    TTTGATAATG TTGAACTTTA CGGTGAGGCC GGTGAACTCC GTGATGACCC TCCAATCATT
    GTCATTGAGT TGTATGATCA AGACACTGTG GGGAAAGCTG AGTTTATTGG CCGAACATTT
    GCAAAACCAC TTACTAAGAT GGTTGATGAA CACTACGGCC CACCGCGGTT TCCTCCCCAG
    CTCGAGTATT ATCAGATCTA CCGAGGAAAC TGTGCTGCGG GAGATCTATT GGCTGCTTTT
    GAGCTTCTAC AGATTCCTTA TGACGATGAG GAGATCAGAA GAGCTCTTAT TGCTGTCCAT
    GACTTTGCTG TGCCTCAGAT CAAGATTGGT CCAGCAGGGC GGGCAGCACT TCCTCCAATC
    GATGGACCGA CCGATTCTGA CCGTGGGCCC ATTCTGCCTG TTCCATTGGG AATTCGACCA
    GTTCTCAGCA GATATCGCAT CGAGGTCCTG TTTTGGGGTC TGAGGGACCT TAAAAGGGTC
    AACCTGGCTC AGGTGGACCG ACCTCGTGTG GACATTGAGT GTGCAGGGAA AGGAGTTCAG
    TCAGCCCTAA TTCAAAACTA CAAGAAGAAT CCAAACTTCA GCACATTAGT AAAGTGGTTT
    GAAGTGGATC TGCCAGAAAA TGAGCTGCTC CATCCACCAC TTAATATTCG GGTAGTGGAT
    TGCAGGGCAT TCGGACGCTA CATTTTGGTG GGGTCTCATG CTGTGACCAC CCTTCGCAAG
    TTCATCTACA GCCCCCCAGA CAAGACTGCT AACAACTGGG CTCACACAGG TGACATTGTG
    GTCAACATGA GCCCTGAACC TAACATTAAG AAGATGGACA CGGTTGTCAA GATTGAAGCT
    ACCACCGATG CGGTTGTTAA AGTTGACTTG AATGAGGATG AGAAGGAGAA AGAAAAGAAG
    AAGAAGAAGA AGAAGAAAGG AGAAGAAGTG GAGGAAGAGG AGCCGGATGA GAGCATGCTG
    GACTGGTGGT CCAAATACTT TGCCTCAATA GAGACTATGA TGGAGAATCT AAGAGCTCAG
    GAGGCCGCTC AGGCAGAGGC AGAGGAAAGA GAAGATTTGG AGATTGCAGC AGAGAGTGCA
    GAGATCAAAG CTGATGACTT TCCTATGAAA GGCACCAAAC CTAAAGAGAA GAGCAAAGAC
    AAGAAGAGCA CCAAGGACAA AAAGAAGAAC AACGATGGCA CAGAAAAACG ACCTCCAAAA
    CCAAAAGTTG ATGAACTCAT GGTGTACAAT AAAGAGCTGG AGAGTGAGTT CGGTAGCTTT
    GAGGACTGGC TTCACACCTT CAACCTGTAC CGAGGAAAGG CTGGAGATGA TGATGACCAC
    AATGTGGTTG ATGAGGACAG AATTGTGGGC AGATTCAAGG GCTCCCTCTG TATGTATAAA
    CTACCACTGT CTGAGGAGAT CACCAGAGAG GCAGGATTTG ATCCAAACAT GGGCATGTTC
    CAGAGTATTC CTCACAATGA CCCTATCAAC GTTCTTGTCA GGATTTATAT CATTCGAGCC
    ACAGACCTGC ATCCTGCAGA TATAAATGGA AAGGCCGACC CTTATATAGT CATTAAATTG
    GGAAAGTCAG ACATTCGAGA CAAGGAGAAC TACATATCCA AGCAGCTGAA TCCTGTCTTT
    GGAAAGTCAT TTGATATAGA GGCCACGTTC CCAATGGAGT CTATGCTGAC TGTGGCAGTG
    TATGACTGGG ATTTAGTCGG AACTGATGAC CTGATTGGTG AGACTAAGAT TGATTTGGAG
    AACAGATACT ACAGCAAACA CAGAGCCACA TGTGGCATTG CTTCCAACTA CTCTGTCCAT
    GGTTATAATG TATGGAGAGA TCCTCAGAAA CCAGCTCAGA TCCTGGCTAA GTTGTGCAAA
    GAAGGTAAAC TGGATGGACC TCACTATGGA CCTGGGGGAA GAGTCAAAGT TGCAAACCGG
    ATCTTTCTTG GGCCGACAGA AATTGAGGAT GAGAGTGGTC TGAAAAAACA AACAGAAGAA
    CACTTGGCGC TCACAGTCCT TAGACATTGG GAGGAAATCC CACGTGTTGG TTGCAAACTC
    ATCCCTGAGC ATGTGGAGAC GAGGCCTCTC CTCAACCCAG ACAAGCCAGG CATAGAGCAG
    GGAAGGATTG AAATGTGGGT GGACATGTTT CCTATGGATG TCCCAGCACC AGGACCAGCC
    ATTGATATAT CGCCACGCAA ACCAAAGAGA TATGAGCTCA GGGTGATAAT ATGGAATACT
    GACGAGGTAA TTCTGGAAGA CGATGATTAC TTCACAGGGG AAAAGTCCAG TGACATTTTT
    GTGAGGGGCT GGCTTAAAGG ACAGCAGGAG GACAAACAGG ACACCGATGT ACATTATCAC
    TCTCTAACTG GAGAGGGAAA CTTCAACTGG CGCTTTGTCT TCCCCTTTGA CTATCTCATG
    GCCGAGGAGA AGATTGTTAT CTCCAAGAAG GAATCAATGT TTTCCTGGGA TGAGACTGAG
    TATAAGATCC CTGCTCGACT CACCCTTCAA GTATGGGATG CCGATCACTT TTCTGCAGAT
    GATTTTCTGG GTGCGATTGA GTTGGACTTG AATAAGTTTC CGCGTGGGGC AAAAACAGCC
    AAGCAGTGCT CTCTAGACAT GGTTCTCAAA GAGCATGAGC TGCCAACCAT CTCCATCTTC
    AAACAAAAGA GAGTCAAGGG CTGGTGGCCA TTTGTGGCCC AAAATGAGAA TGATGAATTT
    GAGCTAACGG GAAAAGTAGA GGCAGAGCTC CATCTGTTGA CAGCAGAGGA GGCAGAGAAG
    AGTCCAGTCG GCCTGGGGAG GAATGATCCG GAGCCACTTG AGAAACCAAA TCGTCCAACT
    ACCAGCCTGT CGTGGCTGTT GAGTCCGATT CGGGTGACCA GCATAATGTG TTGGCGGCAG
    TATAAGTTGG TCACAGTGGT GACTCTGCTC ATCATAACCG GGCTGATCTT TTTGGGCCTC
    CTGTTCTTTT CTCTGCCTTC AGCTTTCATG CAGAAGCTCT TCAACATCTC TGGATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42611
    Clone ID Related Accession (Same CDS sequence) XM_009294740.2 , XM_009294740.3
    Accession Version XM_009294740.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6057bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product otoferlin isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009294740.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGGCTTTAG TGGTGCATCT GAAGACTGTC ACGGAGCTCC GTGGGAAGGG GGACCGAATC 
    GCTAAAGTCA CTTTCAGAGG TCTCTCCTTC TTCTCTCGGG TGTTGGAAAA CTGCGAGGAT
    GAAGCTCGAT TTGAGCAGGC GTTCCGATGG CCAATTGGGA GTCAAGTGGA TGGCGACGAG
    ATGCTGGAGA TCCAAGTGTT TAATTACAGC AAAGTCTTCA CTAATAGGTT AATCGGCACA
    TTCCGCATGG TTCTTCAGAA GGTGGTGGAG GAGGGACACC TGGAGGTGTC TGACACACTC
    ATTGACGACA ATAACACAGC CATACAGACA ACCATTTCCA TAGAGATCAA ATATCAGACC
    ATGGACGGGA GTGTAAAGGT GTGGAGCGAT GGTGAATTCC TTGATATTCC AGATGACCTT
    GATGGAACCT TCCAGTTTGA GACAGATAGT CTTCTCTCAG GACGCAGCCA GAGTTCTGGA
    ACTTCACCTG GACGATCCAT TCATGGCATT CCCACATTCC GCAAGGCAGG AAAAGGAGTG
    TTCTCAGCCA TGAAGCTGGG CAAGACCCGC ACCTCTAAAG ATGACCACAA AAAAGGAGAT
    GATGCTGCCA TTCTGGATGC AGAAGATCTG GATCGAAAAG CCATGCGTCT AGGCGGCATT
    CTTGACCCTG ATACAATCTC ACTGGCCTCG GTTACTGCAG TGACCACTAA TGTCTCCAAC
    AAAAGATCAA AGCCTGACAT TAAAATGGAG CCGAGTTCAG GGCGTCCGGT TGATTATCAG
    ATCAGTGTTA CAGTAATCGA GGCCCGTCAG CTGGTCGGAT TGAACATGGA CCCTGTGGTT
    TGCGTGGAAA TTGGAGAGGA GAAGAAGTAC ACGTCAATGA AGGAGTCAAC AAACTGCCCT
    TACTACAATG AGTATTTTGT CTTCGACTTC CACGTGCCTC CAGACGTCAT GTTTGACAAA
    ATTATAAAGA TTTCAGTAAT ACATTCTAAA AACCTTCTGC GAAGCGGCAC CCTCGTGGGC
    ACCTTCAAAC TAGACGTGGG GACTGTTTAC ACACAACCTG AACATCAGTT TCATCACAAA
    TGGGCGATGC TGTCAGACCC TGATGACATC ACGACTGGCT GCAAAGGTTA TGTTAAATGT
    GACATCGCAG TCGTGGGAAA AGGAGACAAC ATCAAGACTC CACACAAGGC CAGTGAAGCA
    GACGAGGATG ACATAGAGGG GAATCTTCTC TTGCCGGAGG GTGTTCCATC AGAGAGACAG
    TGGGCTCGGT TTTATGTTAA GATTTATAGA GCTGAGGGTC TGCCTAAAAT GAACACCAGT
    ATCATGGCCA ACGTGAAGAA AGCCTTTATT GGGGAAAACA GGGACCTTGT GGACCCTTAC
    GTCCTGGTGC AATTTGCAGG GCAAAAGGGC AAAACATCTG TTCAGAAGAG CAGCTACGAG
    CCCATCTGGA ACGAGCAAGT CATCTTCACG GAGATGTTCC CACCTTTGTG TAGGCGACTG
    AAAGTTCAGA TCCGTGATTC AGACAAGGTG AACGATGTTG CCATAGGAAC CCACTTCATT
    GATCTACGAA AGGTTTCCAA TGATGGAGAC AAAGGTTTCT TGCCCACTAT GGGTCCGGCT
    TGGGTGAATA TGTATGGGTC GACCCGTAAC TACACCCTGA TGGACGAACA CCAGGACCTG
    AATGAGGGGT TGGGGGAAGG GGTGTCCTTC AGAGCACGTC TGCTCATTAG TATCGCGGTG
    GAGATTCTAG ACACCACCTC TGCTGAGATC ATGAGCTCCA CTGAAGTACA CATGGAGCCG
    GTGTCCAACA TCTCAGAGAG TGCTACAGGA AAGATGGAAG AATTTTTCCT TTTCGGGTCA
    TTCTTGGAGG CCACAATGAT AGACAGAAAA ATAGGAGATA AACCCATCAG CTTTGAAGTC
    ACCATCGGTA ACTATGGTAA CCAGATTGAT GGTGTGAGCA AGCCTGCATT AGCGAAGAAG
    AAGAAGGAGG GTGGAGGAGA GAGTGAAGAA GAGGAGTCTG AGCTTATCCA CAACTCCAGC
    GAGGAAGAGG CAGAGGATGA TGGAGATCTG ACCTCTGTAC CGTCTACACC TCCTATGAAG
    CCTGTTATCA CCGACAGAAA TTACTTCCAC TTGCCTTACT TTGAAAAGAA ACCCTGCATT
    TACATCAAGA GCTGGTGGCA GGACCAGCGG AGACGTCTCT ACAACTCCAA CATAATGGAC
    AAGATAGCTG ATAAACTGGA GGAAGGTCTG AATGATGTTC AAGAGATCAT AAAAACAGAG
    AAGGCATATC CAGAACGAAG ACTAAGAGGT GTATTAGAAG AACTTAGCAC AAGCTGCAGC
    CGGTTTGTTA CACTTGCAAA CAAGGATCAG AATCTTTCTG GAAGAACCAA GCTGGACAGA
    GAGAGGCTGA AGTCCTGTAT GAGAGAAATG GAGAGTATGG GTCAGCAGGC GAAGACCATC
    CGCACACAAG TGAAAAGAAA CACAGTTCGA GATAAACTCA AGCTAGTGTT GAATTTCCTT
    CAGAGGCTTC GATTCTTAGC AGACGAGCCC CAGCACAGCA TCCCTGATGT GTTCATATGG
    ATGATTAGCA ACAACAAACG CATCGCATAC GCCCGCATAC CCTCCAAAGA CATCCTCTAC
    TCAATTGTCG ATGAAGAGAT GGGCAAAGAC TGTGGAAAAG TTAAAGCTGT TTTTCTCAGG
    CTGCCTGGAA AGAAAGGTTT TGGGCCTGCT GGCTGGACAG TTCAGGCTAA ATTAGAGATG
    TATTTGTGGC TGGGCCTGAA TAAACAGAGG AAAGACTTCC TGATTGGCCT CCCTAGTGGC
    TTTGAAGAAA ACAAGGCAGT AAAGGGAATT GGCATACAAG CTGTCCCTCC CATCAGCCTA
    GTTTATAACA TGAAGCAGGT GTTTCAGCTG AGGGCCCACA TGTATCAAGC TCGCAGTCTG
    TTTGCTGCGG ACACTAGTGG CCTGTCTGAT CCATTTGCAA GGGTTTTCTT CTCCACCCAC
    AGCCAGGTCA CGGAGGTCCT CAGTGAGACT CTCTGTCCAA CATGGGATCA GCTGCTTGTG
    TTTGATAATG TTGAACTTTA CGGTGAGGCC GGTGAACTCC GTGATGACCC TCCAATCATT
    GTCATTGAGT TGTATGATCA AGACACTGTG GGGAAAGCTG AGTTTATTGG CCGAACATTT
    GCAAAACCAC TTACTAAGAT GGTTGATGAA CACTACGGCC CACCGCGGTT TCCTCCCCAG
    CTCGAGTATT ATCAGATCTA CCGAGGAAAC TGTGCTGCGG GAGATCTATT GGCTGCTTTT
    GAGCTTCTAC AGATTCCTTA TGACGATGAG GAGATCAGAA GAGCTCTTAT TGCTGTCCAT
    GACTTTGCTG TGCCTCAGAT CAAGATTGGT CCAGCAGGGC GGGCAGCACT TCCTCCAATC
    GATGGACCGA CCGATTCTGA CCGTGGGCCC ATTCTGCCTG TTCCATTGGG AATTCGACCA
    GTTCTCAGCA GATATCGCAT CGAGGTCCTG TTTTGGGGTC TGAGGGACCT TAAAAGGGTC
    AACCTGGCTC AGGTGGACCG ACCTCGTGTG GACATTGAGT GTGCAGGGAA AGGAGTTCAG
    TCAGCCCTAA TTCAAAACTA CAAGAAGAAT CCAAACTTCA GCACATTAGT AAAGTGGTTT
    GAAGTGGATC TGCCAGAAAA TGAGCTGCTC CATCCACCAC TTAATATTCG GGTAGTGGAT
    TGCAGGGCAT TCGGACGCTA CATTTTGGTG GGGTCTCATG CTGTGACCAC CCTTCGCAAG
    TTCATCTACA GCCCCCCAGA CAAGACTGCT AACAACTGGG CTCACACAGG TGACATTGTG
    GTCAACATGA GCCCTGAACC TAACATTAAG AAGATGGACA CGGTTGTCAA GATTGAAGCT
    ACCACCGATG CGGTTGTTAA AGTTGACTTG AATGAGGATG AGAAGGAGAA AGAAAAGAAG
    AAGAAGAAGA AGAAGAAAGG AGAAGAAGTG GAGGAAGAGG AGCCGGATGA GAGCATGCTG
    GACTGGTGGT CCAAATACTT TGCCTCAATA GAGACTATGA TGGAGAATCT AAGAGCTCAG
    GAGGCCGCTC AGGCAGAGGC AGAGGAAAGA GAAGATTTGG AGATTGCAGC AGAGAGTGCA
    GAGATCAAAG CTGATGACTT TCCTATGAAA GGCACCAAAC CTAAAGAGAA GAGCAAAGAC
    AAGAAGAGCA CCAAGGACAA AAAGAAGAAC AACGATGGCA CAGAAAAACG ACCTCCAAAA
    CCAAAAGTTG ATGAACTCAT GGTGTACAAT AAAGAGCTGG AGAGTGAGTT CGGTAGCTTT
    GAGGACTGGC TTCACACCTT CAACCTGTAC CGAGGAAAGG CTGGAGATGA TGATGACCAC
    AATGTGGTTG ATGAGGACAG AATTGTGGGC AGATTCAAGG GCTCCCTCTG TATGTATAAA
    CTACCACTGT CTGAGGAGAT CACCAGAGAG GCAGGATTTG ATCCAAACAT GGGCATGTTC
    CAGAGTATTC CTCACAATGA CCCTATCAAC GTTCTTGTCA GGATTTATAT CATTCGAGCC
    ACAGACCTGC ATCCTGCAGA TATAAATGGA AAGGCCGACC CTTATATAGT CATTAAATTG
    GGAAAGTCAG ACATTCGAGA CAAGGAGAAC TACATATCCA AGCAGCTGAA TCCTGTCTTT
    GGAAAGTCAT TTGATATAGA GGCCACGTTC CCAATGGAGT CTATGCTGAC TGTGGCAGTG
    TATGACTGGG ATTTAGTCGG AACTGATGAC CTGATTGGTG AGACTAAGAT TGATTTGGAG
    AACAGATACT ACAGCAAACA CAGAGCCACA TGTGGCATTG CTTCCAACTA CTCTGTCCAT
    GGTTATAATG TATGGAGAGA TCCTCAGAAA CCAGCTCAGA TCCTGGCTAA GTTGTGCAAA
    GAAGGTAAAC TGGATGGACC TCACTATGGA CCTGGGGGAA GAGTCAAAGT TGCAAACCGG
    ATCTTTCTTG GGCCGACAGA AATTGAGGAT GAGAGTGGTC TGAAAAAACA AACAGAAGAA
    CACTTGGCGC TCACAGTCCT TAGACATTGG GAGGAAATCC CACGTGTTGG TTGCAAACTC
    ATCCCTGAGC ATGTGGAGAC GAGGCCTCTC CTCAACCCAG ACAAGCCAGG CATAGAGCAG
    GGAAGGATTG AAATGTGGGT GGACATGTTT CCTATGGATG TCCCAGCACC AGGACCAGCC
    ATTGATATAT CGCCACGCAA ACCAAAGAGA TATGAGCTCA GGGTGATAAT ATGGAATACT
    GACGAGGTAA TTCTGGAAGA CGATGATTAC TTCACAGGGG AAAAGTCCAG TGACATTTTT
    GTGAGGGGCT GGCTTAAAGG ACAGCAGGAG GACAAACAGG ACACCGATGT ACATTATCAC
    TCTCTAACTG GAGAGGGAAA CTTCAACTGG CGCTTTGTCT TCCCCTTTGA CTATCTCATG
    GCCGAGGAGA AGATTGTTAT CTCCAAGAAG GAATCAATGT TTTCCTGGGA TGAGACTGAG
    TATAAGATCC CTGCTCGACT CACCCTTCAA GTATGGGATG CCGATCACTT TTCTGCAGAT
    GATTTTCTGG GTGCGATTGA GTTGGACTTG AATAAGTTTC CGCGTGGGGC AAAAACAGCC
    AAGCAGTGCT CTCTAGACAT GGTTCTCAAA GAGCATGAGC TGCCAACCAT CTCCATCTTC
    AAACAAAAGA GAGTCAAGGG CTGGTGGCCA TTTGTGGCCC AAAATGAGAA TGATGAATTT
    GAGCTAACGG GAAAAGTAGA GGCAGAGCTC CATCTGTTGA CAGCAGAGGA GGCAGAGAAG
    AGTCCAGTCG GCCTGGGGAG GAATGATCCG GAGCCACTTG AGAAACCAAA TCGTCCAACT
    ACCAGCCTGT CGTGGCTGTT GAGTCCGATT CGGGTGACCA GCATAATGTG TTGGCGGCAG
    TATAAGTTGG TCACAGTGGT GACTCTGCTC ATCATAACCG GGCTGATCTT TTTGGGCCTC
    CTGTTCTTTT CTCTGCCTTC AGCTTTCATG CAGAAGCTCT TCAACATCTC TGGATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009293015.1
    CDS300..6356
    Translation

    Target ORF information:

    RefSeq Version XM_009294740.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio otoferlin a (otofa), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009294740.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGGCTTTAG TGGTGCATCT GAAGACTGTC ACGGAGCTCC GTGGGAAGGG GGACCGAATC 
    GCTAAAGTCA CTTTCAGAGG TCTCTCCTTC TTCTCTCGGG TGTTGGAAAA CTGCGAGGAT
    GAAGCTCGAT TTGAGCAGGC GTTCCGATGG CCAATTGGGA GTCAAGTGGA TGGCGACGAG
    ATGCTGGAGA TCCAAGTGTT TAATTACAGC AAAGTCTTCA CTAATAGGTT AATCGGCACA
    TTCCGCATGG TTCTTCAGAA GGTGGTGGAG GAGGGACACC TGGAGGTGTC TGACACACTC
    ATTGACGACA ATAACACAGC CATACAGACA ACCATTTCCA TAGAGATCAA ATATCAGACC
    ATGGACGGGA GTGTAAAGGT GTGGAGCGAT GGTGAATTCC TTGATATTCC AGATGACCTT
    GATGGAACCT TCCAGTTTGA GACAGATAGT CTTCTCTCAG GACGCAGCCA GAGTTCTGGA
    ACTTCACCTG GACGATCCAT TCATGGCATT CCCACATTCC GCAAGGCAGG AAAAGGAGTG
    TTCTCAGCCA TGAAGCTGGG CAAGACCCGC ACCTCTAAAG ATGACCACAA AAAAGGAGAT
    GATGCTGCCA TTCTGGATGC AGAAGATCTG GATCGAAAAG CCATGCGTCT AGGCGGCATT
    CTTGACCCTG ATACAATCTC ACTGGCCTCG GTTACTGCAG TGACCACTAA TGTCTCCAAC
    AAAAGATCAA AGCCTGACAT TAAAATGGAG CCGAGTTCAG GGCGTCCGGT TGATTATCAG
    ATCAGTGTTA CAGTAATCGA GGCCCGTCAG CTGGTCGGAT TGAACATGGA CCCTGTGGTT
    TGCGTGGAAA TTGGAGAGGA GAAGAAGTAC ACGTCAATGA AGGAGTCAAC AAACTGCCCT
    TACTACAATG AGTATTTTGT CTTCGACTTC CACGTGCCTC CAGACGTCAT GTTTGACAAA
    ATTATAAAGA TTTCAGTAAT ACATTCTAAA AACCTTCTGC GAAGCGGCAC CCTCGTGGGC
    ACCTTCAAAC TAGACGTGGG GACTGTTTAC ACACAACCTG AACATCAGTT TCATCACAAA
    TGGGCGATGC TGTCAGACCC TGATGACATC ACGACTGGCT GCAAAGGTTA TGTTAAATGT
    GACATCGCAG TCGTGGGAAA AGGAGACAAC ATCAAGACTC CACACAAGGC CAGTGAAGCA
    GACGAGGATG ACATAGAGGG GAATCTTCTC TTGCCGGAGG GTGTTCCATC AGAGAGACAG
    TGGGCTCGGT TTTATGTTAA GATTTATAGA GCTGAGGGTC TGCCTAAAAT GAACACCAGT
    ATCATGGCCA ACGTGAAGAA AGCCTTTATT GGGGAAAACA GGGACCTTGT GGACCCTTAC
    GTCCTGGTGC AATTTGCAGG GCAAAAGGGC AAAACATCTG TTCAGAAGAG CAGCTACGAG
    CCCATCTGGA ACGAGCAAGT CATCTTCACG GAGATGTTCC CACCTTTGTG TAGGCGACTG
    AAAGTTCAGA TCCGTGATTC AGACAAGGTG AACGATGTTG CCATAGGAAC CCACTTCATT
    GATCTACGAA AGGTTTCCAA TGATGGAGAC AAAGGTTTCT TGCCCACTAT GGGTCCGGCT
    TGGGTGAATA TGTATGGGTC GACCCGTAAC TACACCCTGA TGGACGAACA CCAGGACCTG
    AATGAGGGGT TGGGGGAAGG GGTGTCCTTC AGAGCACGTC TGCTCATTAG TATCGCGGTG
    GAGATTCTAG ACACCACCTC TGCTGAGATC ATGAGCTCCA CTGAAGTACA CATGGAGCCG
    GTGTCCAACA TCTCAGAGAG TGCTACAGGA AAGATGGAAG AATTTTTCCT TTTCGGGTCA
    TTCTTGGAGG CCACAATGAT AGACAGAAAA ATAGGAGATA AACCCATCAG CTTTGAAGTC
    ACCATCGGTA ACTATGGTAA CCAGATTGAT GGTGTGAGCA AGCCTGCATT AGCGAAGAAG
    AAGAAGGAGG GTGGAGGAGA GAGTGAAGAA GAGGAGTCTG AGCTTATCCA CAACTCCAGC
    GAGGAAGAGG CAGAGGATGA TGGAGATCTG ACCTCTGTAC CGTCTACACC TCCTATGAAG
    CCTGTTATCA CCGACAGAAA TTACTTCCAC TTGCCTTACT TTGAAAAGAA ACCCTGCATT
    TACATCAAGA GCTGGTGGCA GGACCAGCGG AGACGTCTCT ACAACTCCAA CATAATGGAC
    AAGATAGCTG ATAAACTGGA GGAAGGTCTG AATGATGTTC AAGAGATCAT AAAAACAGAG
    AAGGCATATC CAGAACGAAG ACTAAGAGGT GTATTAGAAG AACTTAGCAC AAGCTGCAGC
    CGGTTTGTTA CACTTGCAAA CAAGGATCAG AATCTTTCTG GAAGAACCAA GCTGGACAGA
    GAGAGGCTGA AGTCCTGTAT GAGAGAAATG GAGAGTATGG GTCAGCAGGC GAAGACCATC
    CGCACACAAG TGAAAAGAAA CACAGTTCGA GATAAACTCA AGCTAGTGTT GAATTTCCTT
    CAGAGGCTTC GATTCTTAGC AGACGAGCCC CAGCACAGCA TCCCTGATGT GTTCATATGG
    ATGATTAGCA ACAACAAACG CATCGCATAC GCCCGCATAC CCTCCAAAGA CATCCTCTAC
    TCAATTGTCG ATGAAGAGAT GGGCAAAGAC TGTGGAAAAG TTAAAGCTGT TTTTCTCAGG
    CTGCCTGGAA AGAAAGGTTT TGGGCCTGCT GGCTGGACAG TTCAGGCTAA ATTAGAGATG
    TATTTGTGGC TGGGCCTGAA TAAACAGAGG AAAGACTTCC TGATTGGCCT CCCTAGTGGC
    TTTGAAGAAA ACAAGGCAGT AAAGGGAATT GGCATACAAG CTGTCCCTCC CATCAGCCTA
    GTTTATAACA TGAAGCAGGT GTTTCAGCTG AGGGCCCACA TGTATCAAGC TCGCAGTCTG
    TTTGCTGCGG ACACTAGTGG CCTGTCTGAT CCATTTGCAA GGGTTTTCTT CTCCACCCAC
    AGCCAGGTCA CGGAGGTCCT CAGTGAGACT CTCTGTCCAA CATGGGATCA GCTGCTTGTG
    TTTGATAATG TTGAACTTTA CGGTGAGGCC GGTGAACTCC GTGATGACCC TCCAATCATT
    GTCATTGAGT TGTATGATCA AGACACTGTG GGGAAAGCTG AGTTTATTGG CCGAACATTT
    GCAAAACCAC TTACTAAGAT GGTTGATGAA CACTACGGCC CACCGCGGTT TCCTCCCCAG
    CTCGAGTATT ATCAGATCTA CCGAGGAAAC TGTGCTGCGG GAGATCTATT GGCTGCTTTT
    GAGCTTCTAC AGATTCCTTA TGACGATGAG GAGATCAGAA GAGCTCTTAT TGCTGTCCAT
    GACTTTGCTG TGCCTCAGAT CAAGATTGGT CCAGCAGGGC GGGCAGCACT TCCTCCAATC
    GATGGACCGA CCGATTCTGA CCGTGGGCCC ATTCTGCCTG TTCCATTGGG AATTCGACCA
    GTTCTCAGCA GATATCGCAT CGAGGTCCTG TTTTGGGGTC TGAGGGACCT TAAAAGGGTC
    AACCTGGCTC AGGTGGACCG ACCTCGTGTG GACATTGAGT GTGCAGGGAA AGGAGTTCAG
    TCAGCCCTAA TTCAAAACTA CAAGAAGAAT CCAAACTTCA GCACATTAGT AAAGTGGTTT
    GAAGTGGATC TGCCAGAAAA TGAGCTGCTC CATCCACCAC TTAATATTCG GGTAGTGGAT
    TGCAGGGCAT TCGGACGCTA CATTTTGGTG GGGTCTCATG CTGTGACCAC CCTTCGCAAG
    TTCATCTACA GCCCCCCAGA CAAGACTGCT AACAACTGGG CTCACACAGG TGACATTGTG
    GTCAACATGA GCCCTGAACC TAACATTAAG AAGATGGACA CGGTTGTCAA GATTGAAGCT
    ACCACCGATG CGGTTGTTAA AGTTGACTTG AATGAGGATG AGAAGGAGAA AGAAAAGAAG
    AAGAAGAAGA AGAAGAAAGG AGAAGAAGTG GAGGAAGAGG AGCCGGATGA GAGCATGCTG
    GACTGGTGGT CCAAATACTT TGCCTCAATA GAGACTATGA TGGAGAATCT AAGAGCTCAG
    GAGGCCGCTC AGGCAGAGGC AGAGGAAAGA GAAGATTTGG AGATTGCAGC AGAGAGTGCA
    GAGATCAAAG CTGATGACTT TCCTATGAAA GGCACCAAAC CTAAAGAGAA GAGCAAAGAC
    AAGAAGAGCA CCAAGGACAA AAAGAAGAAC AACGATGGCA CAGAAAAACG ACCTCCAAAA
    CCAAAAGTTG ATGAACTCAT GGTGTACAAT AAAGAGCTGG AGAGTGAGTT CGGTAGCTTT
    GAGGACTGGC TTCACACCTT CAACCTGTAC CGAGGAAAGG CTGGAGATGA TGATGACCAC
    AATGTGGTTG ATGAGGACAG AATTGTGGGC AGATTCAAGG GCTCCCTCTG TATGTATAAA
    CTACCACTGT CTGAGGAGAT CACCAGAGAG GCAGGATTTG ATCCAAACAT GGGCATGTTC
    CAGAGTATTC CTCACAATGA CCCTATCAAC GTTCTTGTCA GGATTTATAT CATTCGAGCC
    ACAGACCTGC ATCCTGCAGA TATAAATGGA AAGGCCGACC CTTATATAGT CATTAAATTG
    GGAAAGTCAG ACATTCGAGA CAAGGAGAAC TACATATCCA AGCAGCTGAA TCCTGTCTTT
    GGAAAGTCAT TTGATATAGA GGCCACGTTC CCAATGGAGT CTATGCTGAC TGTGGCAGTG
    TATGACTGGG ATTTAGTCGG AACTGATGAC CTGATTGGTG AGACTAAGAT TGATTTGGAG
    AACAGATACT ACAGCAAACA CAGAGCCACA TGTGGCATTG CTTCCAACTA CTCTGTCCAT
    GGTTATAATG TATGGAGAGA TCCTCAGAAA CCAGCTCAGA TCCTGGCTAA GTTGTGCAAA
    GAAGGTAAAC TGGATGGACC TCACTATGGA CCTGGGGGAA GAGTCAAAGT TGCAAACCGG
    ATCTTTCTTG GGCCGACAGA AATTGAGGAT GAGAGTGGTC TGAAAAAACA AACAGAAGAA
    CACTTGGCGC TCACAGTCCT TAGACATTGG GAGGAAATCC CACGTGTTGG TTGCAAACTC
    ATCCCTGAGC ATGTGGAGAC GAGGCCTCTC CTCAACCCAG ACAAGCCAGG CATAGAGCAG
    GGAAGGATTG AAATGTGGGT GGACATGTTT CCTATGGATG TCCCAGCACC AGGACCAGCC
    ATTGATATAT CGCCACGCAA ACCAAAGAGA TATGAGCTCA GGGTGATAAT ATGGAATACT
    GACGAGGTAA TTCTGGAAGA CGATGATTAC TTCACAGGGG AAAAGTCCAG TGACATTTTT
    GTGAGGGGCT GGCTTAAAGG ACAGCAGGAG GACAAACAGG ACACCGATGT ACATTATCAC
    TCTCTAACTG GAGAGGGAAA CTTCAACTGG CGCTTTGTCT TCCCCTTTGA CTATCTCATG
    GCCGAGGAGA AGATTGTTAT CTCCAAGAAG GAATCAATGT TTTCCTGGGA TGAGACTGAG
    TATAAGATCC CTGCTCGACT CACCCTTCAA GTATGGGATG CCGATCACTT TTCTGCAGAT
    GATTTTCTGG GTGCGATTGA GTTGGACTTG AATAAGTTTC CGCGTGGGGC AAAAACAGCC
    AAGCAGTGCT CTCTAGACAT GGTTCTCAAA GAGCATGAGC TGCCAACCAT CTCCATCTTC
    AAACAAAAGA GAGTCAAGGG CTGGTGGCCA TTTGTGGCCC AAAATGAGAA TGATGAATTT
    GAGCTAACGG GAAAAGTAGA GGCAGAGCTC CATCTGTTGA CAGCAGAGGA GGCAGAGAAG
    AGTCCAGTCG GCCTGGGGAG GAATGATCCG GAGCCACTTG AGAAACCAAA TCGTCCAACT
    ACCAGCCTGT CGTGGCTGTT GAGTCCGATT CGGGTGACCA GCATAATGTG TTGGCGGCAG
    TATAAGTTGG TCACAGTGGT GACTCTGCTC ATCATAACCG GGCTGATCTT TTTGGGCCTC
    CTGTTCTTTT CTCTGCCTTC AGCTTTCATG CAGAAGCTCT TCAACATCTC TGGATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42612
    Clone ID Related Accession (Same CDS sequence) XM_021468442.1
    Accession Version XM_021468442.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6027bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product otoferlin isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGGCTTTAG TGGTGCATCT GAAGACTGTC ACGGAGCTCC GTGGGAAGGG GGACCGAATC 
    GCTAAAGTCA CTTTCAGAGG TCTCTCCTTC TTCTCTCGGG TGTTGGAAAA CTGCGAGGAT
    GAAGCTCGAT TTGAGCAGGC GTTCCGATGG CCAATTGGGA GTCAAGTGGA TGGCGACGAG
    ATGCTGGAGA TCCAAGTGTT TAATTACAGC AAAGTCTTCA CTAATAGGTT AATCGGCACA
    TTCCGCATGG TTCTTCAGAA GGTGGTGGAG GAGGGACACC TGGAGGTGTC TGACACACTC
    ATTGACGACA ATAACACAGC CATACAGACA ACCATTTCCA TAGAGATCAA ATATCAGACC
    ATGGACGGGA GTGTAAAGGT GTGGAGCGAT GGTGAATTCC TTGATATTCC AGATGACCTT
    GATGGAACCT TCCAGTTTGA GACAGATAGT CTTCTCTCAG GACGCAGCCA GAGTTCTGGA
    ACTTCACCTG GACGATCCAT TCATGGCATT CCCACATTCC GCAAGGCAGG AAAAGGAGTG
    TTCTCAGCCA TGAAGCTGGG CAAGACCCGC ACCTCTAAAG ATGACCACAA AAAAGGAGAT
    GATGCTGCCA TTCTGGATGC AGAAGATCTG GATCGAAAAG CCATGCGTCT AGGCGGCATT
    CTTGACCCTG ATACAATCTC ACTGGCCTCG GTTACTGCAG TGACCACTAA TGTCTCCAAC
    AAAAGATCAA AGCCTGACAT TAAAATGGAG CCGAGTTCAG GGCGTCCGGT TGATTATCAG
    ATCAGTGTTA CAGTAATCGA GGCCCGTCAG CTGGTCGGAT TGAACATGGA CCCTGTGGTT
    TGCGTGGAAA TTGGAGAGGA GAAGAAGTAC ACGTCAATGA AGGAGTCAAC AAACTGCCCT
    TACTACAATG AGTATTTTGT CTTCGACTTC CACGTGCCTC CAGACGTCAT GTTTGACAAA
    ATTATAAAGA TTTCAGTAAT ACATTCTAAA AACCTTCTGC GAAGCGGCAC CCTCGTGGGC
    ACCTTCAAAC TAGACGTGGG GACTGTTTAC ACACAACCTG AACATCAGTT TCATCACAAA
    TGGGCGATGC TGTCAGACCC TGATGACATC ACGACTGGCT GCAAAGGTTA TGTTAAATGT
    GACATCGCAG TCGTGGGAAA AGGAGACAAC ATCAAGACTC CACACAAGGC CAGTGAAGCA
    GACGAGGATG ACATAGAGGG GAATCTTCTC TTGCCGGAGG GTGTTCCATC AGAGAGACAG
    TGGGCTCGGT TTTATGTTAA GATTTATAGA GCTGAGGGTC TGCCTAAAAT GAACACCAGT
    ATCATGGCCA ACGTGAAGAA AGCCTTTATT GGGGAAAACA GGGACCTTGT GGACCCTTAC
    GTCCTGGTGC AATTTGCAGG GCAAAAGGGC AAAACATCTG TTCAGAAGAG CAGCTACGAG
    CCCATCTGGA ACGAGCAAGT CATCTTCACG GAGATGTTCC CACCTTTGTG TAGGCGACTG
    AAAGTTCAGA TCCGTGATTC AGACAAGGTG AACGATGTTG CCATAGGAAC CCACTTCATT
    GATCTACGAA AGGTTTCCAA TGATGGAGAC AAAGGTTTCT TGCCCACTAT GGGTCCGGCT
    TGGGTGAATA TGTATGGGTC GACCCGTAAC TACACCCTGA TGGACGAACA CCAGGACCTG
    AATGAGGGGT TGGGGGAAGG GGTGTCCTTC AGAGCACGTC TGCTCATTAG TATCGCGGTG
    GAGATTCTAG ACACCACCTC TGCTGAGATC ATGAGCTCCA CTGAAGTACA CATGGAGCCG
    GTGTCCAACA TCTCAGAGAG TGCTACAGGA AAGATGGAAG AATTTTTCCT TTTCGGGTCA
    TTCTTGGAGG CCACAATGAT AGACAGAAAA ATAGGAGATA AACCCATCAG CTTTGAAGTC
    ACCATCGGTA ACTATGGTAA CCAGATTGAT GGTGTGAGCA AGCCTGCATT AGCGAAGAAG
    AAGAAGGAGG GTGGAGGAGA GAGTGAAGAA GAGGAGTCTG AGCTTATCCA CAACTCCAGC
    GAGGAAGAGG CAGAGGATGA TGGAGATCTG ACCTCTGTAC CGTCTACACC TCCTATGAAG
    CCTGTTATCA CCGACAGAAA TTACTTCCAC TTGCCTTACT TTGAAAAGAA ACCCTGCATT
    TACATCAAGA GCTGGTGGCA GGACCAGCGG AGACGTCTCT ACAACTCCAA CATAATGGAC
    AAGATAGCTG ATAAACTGGA GGAAGGTCTG AATGATGTTC AAGAGATCAT AAAAACAGAG
    AAGGCATATC CAGAACGAAG ACTAAGAGGT GTATTAGAAG AACTTAGCAC AAGCTGCAGC
    CGGTTTGTTA CACTTGCAAA CAAGGATCAG AATCTTTCTG GAAGAACCAA GCTGGACAGA
    GAGAGGCTGA AGTCCTGTAT GAGAGAAATG GAGAGTATGG GTCAGCAGGC GAAGACCATC
    CGCACACAAG TGAAAAGAAA CACAGTTCGA GATAAACTCA AGCTAGTGTT GAATTTCCTT
    CAGAGGCTTC GATTCTTAGC AGACGAGCCC CAGCACAGCA TCCCTGATGT GTTCATATGG
    ATGATTAGCA ACAACAAACG CATCGCATAC GCCCGCATAC CCTCCAAAGA CATCCTCTAC
    TCAATTGTCG ATGAAGAGAT GGGCAAAGAC TGTGGAAAAG TTAAAGCTGT TTTTCTCAGG
    CTGCCTGGAA AGAAAGGTTT TGGGCCTGCT GGCTGGACAG TTCAGGCTAA ATTAGAGATG
    TATTTGTGGC TGGGCCTGAA TAAACAGAGG AAAGACTTCC TGATTGGCCT CCCTAGTGGC
    TTTGAAGAAA ACAAGGCAGT AAAGGGAATT GGCATACAAG CTGTCCCTCC CATCAGCCTA
    GTTTATAACA TGAAGCAGGT GTTTCAGCTG AGGGCCCACA TGTATCAAGC TCGCAGTCTG
    TTTGCTGCGG ACACTAGTGG CCTGTCTGAT CCATTTGCAA GGGTTTTCTT CTCCACCCAC
    AGCCAGGTCA CGGAGGTCCT CAGTGAGACT CTCTGTCCAA CATGGGATCA GCTGCTTGTG
    TTTGATAATG TTGAACTTTA CGGTGAGGCC GGTGAACTCC GTGATGACCC TCCAATCATT
    GTCATTGAGT TGTATGATCA AGACACTGTG GGGAAAGCTG AGTTTATTGG CCGAACATTT
    GCAAAACCAC TTACTAAGAT GGTTGATGAA CACTACGGCC CACCGCGGTT TCCTCCCCAG
    CTCGAGTATT ATCAGATCTA CCGAGGAAAC TGTGCTGCGG GAGATCTATT GGCTGCTTTT
    GAGCTTCTAC AGATTCCTTA TGACGATGAG GAGATCAGAA GAGCTCTTAT TGCTGTCCAT
    GACTTTGCTG TGCCTCAGAT CAAGATTGGT CCAGCAGGGC GGGCAGCACT TCCTCCAATC
    GATGGACCGA CCGATTCTGA CCGTGGGCCC ATTCTGCCTG TTCCATTGGG AATTCGACCA
    GTTCTCAGCA GATATCGCAT CGAGGTCCTG TTTTGGGGTC TGAGGGACCT TAAAAGGGTC
    AACCTGGCTC AGGTGGACCG ACCTCGTGTG GACATTGAGT GTGCAGGGAA AGGAGTTCAG
    TCAGCCCTAA TTCAAAACTA CAAGAAGAAT CCAAACTTCA GCACATTAGT AAAGTGGTTT
    GAAGTGGATC TGCCAGAAAA TGAGCTGCTC CATCCACCAC TTAATATTCG GGTAGTGGAT
    TGCAGGGCAT TCGGACGCTA CATTTTGGTG GGGTCTCATG CTGTGACCAC CCTTCGCAAG
    TTCATCTACA GCCCCCCAGA CAAGACTGCT AACAACTGGG CTCACACAGG TGACATTGTG
    GTCAACATGA GCCCTGAACC TAACATTAAG AAGATGGACA CGGTTGTCAA GATTGAAGCT
    AATGAGGATG AGAAGGAGAA AGAAAAGAAG AAGAAGAAGA AGAAGAAAGG AGAAGAAGTG
    GAGGAAGAGG AGCCGGATGA GAGCATGCTG GACTGGTGGT CCAAATACTT TGCCTCAATA
    GAGACTATGA TGGAGAATCT AAGAGCTCAG GAGGCCGCTC AGGCAGAGGC AGAGGAAAGA
    GAAGATTTGG AGATTGCAGC AGAGAGTGCA GAGATCAAAG CTGATGACTT TCCTATGAAA
    GGCACCAAAC CTAAAGAGAA GAGCAAAGAC AAGAAGAGCA CCAAGGACAA AAAGAAGAAC
    AACGATGGCA CAGAAAAACG ACCTCCAAAA CCAAAAGTTG ATGAACTCAT GGTGTACAAT
    AAAGAGCTGG AGAGTGAGTT CGGTAGCTTT GAGGACTGGC TTCACACCTT CAACCTGTAC
    CGAGGAAAGG CTGGAGATGA TGATGACCAC AATGTGGTTG ATGAGGACAG AATTGTGGGC
    AGATTCAAGG GCTCCCTCTG TATGTATAAA CTACCACTGT CTGAGGAGAT CACCAGAGAG
    GCAGGATTTG ATCCAAACAT GGGCATGTTC CAGAGTATTC CTCACAATGA CCCTATCAAC
    GTTCTTGTCA GGATTTATAT CATTCGAGCC ACAGACCTGC ATCCTGCAGA TATAAATGGA
    AAGGCCGACC CTTATATAGT CATTAAATTG GGAAAGTCAG ACATTCGAGA CAAGGAGAAC
    TACATATCCA AGCAGCTGAA TCCTGTCTTT GGAAAGTCAT TTGATATAGA GGCCACGTTC
    CCAATGGAGT CTATGCTGAC TGTGGCAGTG TATGACTGGG ATTTAGTCGG AACTGATGAC
    CTGATTGGTG AGACTAAGAT TGATTTGGAG AACAGATACT ACAGCAAACA CAGAGCCACA
    TGTGGCATTG CTTCCAACTA CTCTGTCCAT GGTTATAATG TATGGAGAGA TCCTCAGAAA
    CCAGCTCAGA TCCTGGCTAA GTTGTGCAAA GAAGGTAAAC TGGATGGACC TCACTATGGA
    CCTGGGGGAA GAGTCAAAGT TGCAAACCGG ATCTTTCTTG GGCCGACAGA AATTGAGGAT
    GAGAGTGGTC TGAAAAAACA AACAGAAGAA CACTTGGCGC TCACAGTCCT TAGACATTGG
    GAGGAAATCC CACGTGTTGG TTGCAAACTC ATCCCTGAGC ATGTGGAGAC GAGGCCTCTC
    CTCAACCCAG ACAAGCCAGG CATAGAGCAG GGAAGGATTG AAATGTGGGT GGACATGTTT
    CCTATGGATG TCCCAGCACC AGGACCAGCC ATTGATATAT CGCCACGCAA ACCAAAGAGA
    TATGAGCTCA GGGTGATAAT ATGGAATACT GACGAGGTAA TTCTGGAAGA CGATGATTAC
    TTCACAGGGG AAAAGTCCAG TGACATTTTT GTGAGGGGCT GGCTTAAAGG ACAGCAGGAG
    GACAAACAGG ACACCGATGT ACATTATCAC TCTCTAACTG GAGAGGGAAA CTTCAACTGG
    CGCTTTGTCT TCCCCTTTGA CTATCTCATG GCCGAGGAGA AGATTGTTAT CTCCAAGAAG
    GAATCAATGT TTTCCTGGGA TGAGACTGAG TATAAGATCC CTGCTCGACT CACCCTTCAA
    GTATGGGATG CCGATCACTT TTCTGCAGAT GATTTTCTGG GTGCGATTGA GTTGGACTTG
    AATAAGTTTC CGCGTGGGGC AAAAACAGCC AAGCAGTGCT CTCTAGACAT GGTTCTCAAA
    GAGCATGAGC TGCCAACCAT CTCCATCTTC AAACAAAAGA GAGTCAAGGG CTGGTGGCCA
    TTTGTGGCCC AAAATGAGAA TGATGAATTT GAGCTAACGG GAAAAGTAGA GGCAGAGCTC
    CATCTGTTGA CAGCAGAGGA GGCAGAGAAG AGTCCAGTCG GCCTGGGGAG GAATGATCCG
    GAGCCACTTG AGAAACCAAA TCGTCCAACT ACCAGCCTGT CGTGGCTGTT GAGTCCGATT
    CGGGTGACCA GCATAATGTG TTGGCGGCAG TATAAGTTGG TCACAGTGGT GACTCTGCTC
    ATCATAACCG GGCTGATCTT TTTGGGCCTC CTGTTCTTTT CTCTGCCTTC AGCTTTCATG
    CAGAAGCTCT TCAACATCTC TGGATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021324117.1
    CDS300..6326
    Translation

    Target ORF information:

    RefSeq Version XM_021468442.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio otoferlin a (otofa), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021468442.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGGCTTTAG TGGTGCATCT GAAGACTGTC ACGGAGCTCC GTGGGAAGGG GGACCGAATC 
    GCTAAAGTCA CTTTCAGAGG TCTCTCCTTC TTCTCTCGGG TGTTGGAAAA CTGCGAGGAT
    GAAGCTCGAT TTGAGCAGGC GTTCCGATGG CCAATTGGGA GTCAAGTGGA TGGCGACGAG
    ATGCTGGAGA TCCAAGTGTT TAATTACAGC AAAGTCTTCA CTAATAGGTT AATCGGCACA
    TTCCGCATGG TTCTTCAGAA GGTGGTGGAG GAGGGACACC TGGAGGTGTC TGACACACTC
    ATTGACGACA ATAACACAGC CATACAGACA ACCATTTCCA TAGAGATCAA ATATCAGACC
    ATGGACGGGA GTGTAAAGGT GTGGAGCGAT GGTGAATTCC TTGATATTCC AGATGACCTT
    GATGGAACCT TCCAGTTTGA GACAGATAGT CTTCTCTCAG GACGCAGCCA GAGTTCTGGA
    ACTTCACCTG GACGATCCAT TCATGGCATT CCCACATTCC GCAAGGCAGG AAAAGGAGTG
    TTCTCAGCCA TGAAGCTGGG CAAGACCCGC ACCTCTAAAG ATGACCACAA AAAAGGAGAT
    GATGCTGCCA TTCTGGATGC AGAAGATCTG GATCGAAAAG CCATGCGTCT AGGCGGCATT
    CTTGACCCTG ATACAATCTC ACTGGCCTCG GTTACTGCAG TGACCACTAA TGTCTCCAAC
    AAAAGATCAA AGCCTGACAT TAAAATGGAG CCGAGTTCAG GGCGTCCGGT TGATTATCAG
    ATCAGTGTTA CAGTAATCGA GGCCCGTCAG CTGGTCGGAT TGAACATGGA CCCTGTGGTT
    TGCGTGGAAA TTGGAGAGGA GAAGAAGTAC ACGTCAATGA AGGAGTCAAC AAACTGCCCT
    TACTACAATG AGTATTTTGT CTTCGACTTC CACGTGCCTC CAGACGTCAT GTTTGACAAA
    ATTATAAAGA TTTCAGTAAT ACATTCTAAA AACCTTCTGC GAAGCGGCAC CCTCGTGGGC
    ACCTTCAAAC TAGACGTGGG GACTGTTTAC ACACAACCTG AACATCAGTT TCATCACAAA
    TGGGCGATGC TGTCAGACCC TGATGACATC ACGACTGGCT GCAAAGGTTA TGTTAAATGT
    GACATCGCAG TCGTGGGAAA AGGAGACAAC ATCAAGACTC CACACAAGGC CAGTGAAGCA
    GACGAGGATG ACATAGAGGG GAATCTTCTC TTGCCGGAGG GTGTTCCATC AGAGAGACAG
    TGGGCTCGGT TTTATGTTAA GATTTATAGA GCTGAGGGTC TGCCTAAAAT GAACACCAGT
    ATCATGGCCA ACGTGAAGAA AGCCTTTATT GGGGAAAACA GGGACCTTGT GGACCCTTAC
    GTCCTGGTGC AATTTGCAGG GCAAAAGGGC AAAACATCTG TTCAGAAGAG CAGCTACGAG
    CCCATCTGGA ACGAGCAAGT CATCTTCACG GAGATGTTCC CACCTTTGTG TAGGCGACTG
    AAAGTTCAGA TCCGTGATTC AGACAAGGTG AACGATGTTG CCATAGGAAC CCACTTCATT
    GATCTACGAA AGGTTTCCAA TGATGGAGAC AAAGGTTTCT TGCCCACTAT GGGTCCGGCT
    TGGGTGAATA TGTATGGGTC GACCCGTAAC TACACCCTGA TGGACGAACA CCAGGACCTG
    AATGAGGGGT TGGGGGAAGG GGTGTCCTTC AGAGCACGTC TGCTCATTAG TATCGCGGTG
    GAGATTCTAG ACACCACCTC TGCTGAGATC ATGAGCTCCA CTGAAGTACA CATGGAGCCG
    GTGTCCAACA TCTCAGAGAG TGCTACAGGA AAGATGGAAG AATTTTTCCT TTTCGGGTCA
    TTCTTGGAGG CCACAATGAT AGACAGAAAA ATAGGAGATA AACCCATCAG CTTTGAAGTC
    ACCATCGGTA ACTATGGTAA CCAGATTGAT GGTGTGAGCA AGCCTGCATT AGCGAAGAAG
    AAGAAGGAGG GTGGAGGAGA GAGTGAAGAA GAGGAGTCTG AGCTTATCCA CAACTCCAGC
    GAGGAAGAGG CAGAGGATGA TGGAGATCTG ACCTCTGTAC CGTCTACACC TCCTATGAAG
    CCTGTTATCA CCGACAGAAA TTACTTCCAC TTGCCTTACT TTGAAAAGAA ACCCTGCATT
    TACATCAAGA GCTGGTGGCA GGACCAGCGG AGACGTCTCT ACAACTCCAA CATAATGGAC
    AAGATAGCTG ATAAACTGGA GGAAGGTCTG AATGATGTTC AAGAGATCAT AAAAACAGAG
    AAGGCATATC CAGAACGAAG ACTAAGAGGT GTATTAGAAG AACTTAGCAC AAGCTGCAGC
    CGGTTTGTTA CACTTGCAAA CAAGGATCAG AATCTTTCTG GAAGAACCAA GCTGGACAGA
    GAGAGGCTGA AGTCCTGTAT GAGAGAAATG GAGAGTATGG GTCAGCAGGC GAAGACCATC
    CGCACACAAG TGAAAAGAAA CACAGTTCGA GATAAACTCA AGCTAGTGTT GAATTTCCTT
    CAGAGGCTTC GATTCTTAGC AGACGAGCCC CAGCACAGCA TCCCTGATGT GTTCATATGG
    ATGATTAGCA ACAACAAACG CATCGCATAC GCCCGCATAC CCTCCAAAGA CATCCTCTAC
    TCAATTGTCG ATGAAGAGAT GGGCAAAGAC TGTGGAAAAG TTAAAGCTGT TTTTCTCAGG
    CTGCCTGGAA AGAAAGGTTT TGGGCCTGCT GGCTGGACAG TTCAGGCTAA ATTAGAGATG
    TATTTGTGGC TGGGCCTGAA TAAACAGAGG AAAGACTTCC TGATTGGCCT CCCTAGTGGC
    TTTGAAGAAA ACAAGGCAGT AAAGGGAATT GGCATACAAG CTGTCCCTCC CATCAGCCTA
    GTTTATAACA TGAAGCAGGT GTTTCAGCTG AGGGCCCACA TGTATCAAGC TCGCAGTCTG
    TTTGCTGCGG ACACTAGTGG CCTGTCTGAT CCATTTGCAA GGGTTTTCTT CTCCACCCAC
    AGCCAGGTCA CGGAGGTCCT CAGTGAGACT CTCTGTCCAA CATGGGATCA GCTGCTTGTG
    TTTGATAATG TTGAACTTTA CGGTGAGGCC GGTGAACTCC GTGATGACCC TCCAATCATT
    GTCATTGAGT TGTATGATCA AGACACTGTG GGGAAAGCTG AGTTTATTGG CCGAACATTT
    GCAAAACCAC TTACTAAGAT GGTTGATGAA CACTACGGCC CACCGCGGTT TCCTCCCCAG
    CTCGAGTATT ATCAGATCTA CCGAGGAAAC TGTGCTGCGG GAGATCTATT GGCTGCTTTT
    GAGCTTCTAC AGATTCCTTA TGACGATGAG GAGATCAGAA GAGCTCTTAT TGCTGTCCAT
    GACTTTGCTG TGCCTCAGAT CAAGATTGGT CCAGCAGGGC GGGCAGCACT TCCTCCAATC
    GATGGACCGA CCGATTCTGA CCGTGGGCCC ATTCTGCCTG TTCCATTGGG AATTCGACCA
    GTTCTCAGCA GATATCGCAT CGAGGTCCTG TTTTGGGGTC TGAGGGACCT TAAAAGGGTC
    AACCTGGCTC AGGTGGACCG ACCTCGTGTG GACATTGAGT GTGCAGGGAA AGGAGTTCAG
    TCAGCCCTAA TTCAAAACTA CAAGAAGAAT CCAAACTTCA GCACATTAGT AAAGTGGTTT
    GAAGTGGATC TGCCAGAAAA TGAGCTGCTC CATCCACCAC TTAATATTCG GGTAGTGGAT
    TGCAGGGCAT TCGGACGCTA CATTTTGGTG GGGTCTCATG CTGTGACCAC CCTTCGCAAG
    TTCATCTACA GCCCCCCAGA CAAGACTGCT AACAACTGGG CTCACACAGG TGACATTGTG
    GTCAACATGA GCCCTGAACC TAACATTAAG AAGATGGACA CGGTTGTCAA GATTGAAGCT
    AATGAGGATG AGAAGGAGAA AGAAAAGAAG AAGAAGAAGA AGAAGAAAGG AGAAGAAGTG
    GAGGAAGAGG AGCCGGATGA GAGCATGCTG GACTGGTGGT CCAAATACTT TGCCTCAATA
    GAGACTATGA TGGAGAATCT AAGAGCTCAG GAGGCCGCTC AGGCAGAGGC AGAGGAAAGA
    GAAGATTTGG AGATTGCAGC AGAGAGTGCA GAGATCAAAG CTGATGACTT TCCTATGAAA
    GGCACCAAAC CTAAAGAGAA GAGCAAAGAC AAGAAGAGCA CCAAGGACAA AAAGAAGAAC
    AACGATGGCA CAGAAAAACG ACCTCCAAAA CCAAAAGTTG ATGAACTCAT GGTGTACAAT
    AAAGAGCTGG AGAGTGAGTT CGGTAGCTTT GAGGACTGGC TTCACACCTT CAACCTGTAC
    CGAGGAAAGG CTGGAGATGA TGATGACCAC AATGTGGTTG ATGAGGACAG AATTGTGGGC
    AGATTCAAGG GCTCCCTCTG TATGTATAAA CTACCACTGT CTGAGGAGAT CACCAGAGAG
    GCAGGATTTG ATCCAAACAT GGGCATGTTC CAGAGTATTC CTCACAATGA CCCTATCAAC
    GTTCTTGTCA GGATTTATAT CATTCGAGCC ACAGACCTGC ATCCTGCAGA TATAAATGGA
    AAGGCCGACC CTTATATAGT CATTAAATTG GGAAAGTCAG ACATTCGAGA CAAGGAGAAC
    TACATATCCA AGCAGCTGAA TCCTGTCTTT GGAAAGTCAT TTGATATAGA GGCCACGTTC
    CCAATGGAGT CTATGCTGAC TGTGGCAGTG TATGACTGGG ATTTAGTCGG AACTGATGAC
    CTGATTGGTG AGACTAAGAT TGATTTGGAG AACAGATACT ACAGCAAACA CAGAGCCACA
    TGTGGCATTG CTTCCAACTA CTCTGTCCAT GGTTATAATG TATGGAGAGA TCCTCAGAAA
    CCAGCTCAGA TCCTGGCTAA GTTGTGCAAA GAAGGTAAAC TGGATGGACC TCACTATGGA
    CCTGGGGGAA GAGTCAAAGT TGCAAACCGG ATCTTTCTTG GGCCGACAGA AATTGAGGAT
    GAGAGTGGTC TGAAAAAACA AACAGAAGAA CACTTGGCGC TCACAGTCCT TAGACATTGG
    GAGGAAATCC CACGTGTTGG TTGCAAACTC ATCCCTGAGC ATGTGGAGAC GAGGCCTCTC
    CTCAACCCAG ACAAGCCAGG CATAGAGCAG GGAAGGATTG AAATGTGGGT GGACATGTTT
    CCTATGGATG TCCCAGCACC AGGACCAGCC ATTGATATAT CGCCACGCAA ACCAAAGAGA
    TATGAGCTCA GGGTGATAAT ATGGAATACT GACGAGGTAA TTCTGGAAGA CGATGATTAC
    TTCACAGGGG AAAAGTCCAG TGACATTTTT GTGAGGGGCT GGCTTAAAGG ACAGCAGGAG
    GACAAACAGG ACACCGATGT ACATTATCAC TCTCTAACTG GAGAGGGAAA CTTCAACTGG
    CGCTTTGTCT TCCCCTTTGA CTATCTCATG GCCGAGGAGA AGATTGTTAT CTCCAAGAAG
    GAATCAATGT TTTCCTGGGA TGAGACTGAG TATAAGATCC CTGCTCGACT CACCCTTCAA
    GTATGGGATG CCGATCACTT TTCTGCAGAT GATTTTCTGG GTGCGATTGA GTTGGACTTG
    AATAAGTTTC CGCGTGGGGC AAAAACAGCC AAGCAGTGCT CTCTAGACAT GGTTCTCAAA
    GAGCATGAGC TGCCAACCAT CTCCATCTTC AAACAAAAGA GAGTCAAGGG CTGGTGGCCA
    TTTGTGGCCC AAAATGAGAA TGATGAATTT GAGCTAACGG GAAAAGTAGA GGCAGAGCTC
    CATCTGTTGA CAGCAGAGGA GGCAGAGAAG AGTCCAGTCG GCCTGGGGAG GAATGATCCG
    GAGCCACTTG AGAAACCAAA TCGTCCAACT ACCAGCCTGT CGTGGCTGTT GAGTCCGATT
    CGGGTGACCA GCATAATGTG TTGGCGGCAG TATAAGTTGG TCACAGTGGT GACTCTGCTC
    ATCATAACCG GGCTGATCTT TTTGGGCCTC CTGTTCTTTT CTCTGCCTTC AGCTTTCATG
    CAGAAGCTCT TCAACATCTC TGGATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42613
    Clone ID Related Accession (Same CDS sequence) XM_009294742.3 , XM_009294742.2
    Accession Version XM_009294742.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5985bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product otoferlin isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009294742.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGCTTTAG TGGTGCATCT GAAGACTGTC ACGGAGCTCC GTGGGAAGGG GGACCGAATC 
    GCTAAAGTCA CTTTCAGAGG TCTCTCCTTC TTCTCTCGGG TGTTGGAAAA CTGCGAGGAT
    GAAGCTCGAT TTGAGCAGGC GTTCCGATGG CCAATTGGGA GTCAAGTGGA TGGCGACGAG
    ATGCTGGAGA TCCAAGTGTT TAATTACAGC AAAGTCTTCA CTAATAGGTT AATCGGCACA
    TTCCGCATGG TTCTTCAGAA GGTGGTGGAG GAGGGACACC TGGAGGTGTC TGACACACTC
    ATTGACGACA ATAACACAGC CATACAGACA ACCATTTCCA TAGAGATCAA ATATCAGACC
    ATGGACGGGA GTGTAAAGGT GTGGAGCGAT GGTGAATTCC TTGATATTCC AGATGACCTT
    GATGGAACCT TCCAGTTTGA GACAGATAGT CTTCTCTCAG GACGCAGCCA GAGTTCTGGA
    ACTTCACCTG GACGATCCAT TCATGGCATT CCCACATTCC GCAAGGCAGG AAAAGGAGTG
    TTCTCAGCCA TGAAGCTGGG CAAGACCCGC ACCTCTAAAG ATGACCACAA AAAAGGAGAT
    GATGCTGCCA TTCTGGATGC AGAAGATCTG GATCGAAAAG CCATGCGTCT AGGCGGCATT
    CTTGACCCTG ATACAATCTC ACTGGCCTCG GTTACTGCAG TGACCACTAA TGTCTCCAAC
    AAAAGATCAA AGCCTGACAT TAAAATGGAG CCGAGTTCAG GGCGTCCGGT TGATTATCAG
    ATCAGTGTTA CAGTAATCGA GGCCCGTCAG CTGGTCGGAT TGAACATGGA CCCTGTGGTT
    TGCGTGGAAA TTGGAGAGGA GAAGAAGTAC ACGTCAATGA AGGAGTCAAC AAACTGCCCT
    TACTACAATG AGTATTTTGT CTTCGACTTC CACGTGCCTC CAGACGTCAT GTTTGACAAA
    ATTATAAAGA TTTCAGTAAT ACATTCTAAA AACCTTCTGC GAAGCGGCAC CCTCGTGGGC
    ACCTTCAAAC TAGACGTGGG GACTGTTTAC ACACAACCTG AACATCAGTT TCATCACAAA
    TGGGCGATGC TGTCAGACCC TGATGACATC ACGACTGGCT GCAAAGGTTA TGTTAAATGT
    GACATCGCAG TCGTGGGAAA AGGAGACAAC ATCAAGACTC CACACAAGGC CAGTGAAGCA
    GACGAGGATG ACATAGAGGG GAATCTTCTC TTGCCGGAGG GTGTTCCATC AGAGAGACAG
    TGGGCTCGGT TTTATGTTAA GATTTATAGA GCTGAGGGTC TGCCTAAAAT GAACACCAGT
    ATCATGGCCA ACGTGAAGAA AGCCTTTATT GGGGAAAACA GGGACCTTGT GGACCCTTAC
    GTCCTGGTGC AATTTGCAGG GCAAAAGGGC AAAACATCTG TTCAGAAGAG CAGCTACGAG
    CCCATCTGGA ACGAGCAAGT CATCTTCACG GAGATGTTCC CACCTTTGTG TAGGCGACTG
    AAAGTTCAGA TCCGTGATTC AGACAAGGTG AACGATGTTG CCATAGGAAC CCACTTCATT
    GATCTACGAA AGGTTTCCAA TGATGGAGAC AAAGGTTTCT TGCCCACTAT GGGTCCGGCT
    TGGGTGAATA TGTATGGGTC GACCCGTAAC TACACCCTGA TGGACGAACA CCAGGACCTG
    AATGAGGGGT TGGGGGAAGG GGTGTCCTTC AGAGCACGTC TGCTCATTAG TATCGCGGTG
    GAGATTCTAG ACACCACCTC TGCTGAGATC ATGAGCTCCA CTGAAGTACA CATGGAGCCG
    GTGTCCAACA TCTCAGAGAG TGCTACAGGA AAGATGGAAG AATTTTTCCT TTTCGGGTCA
    TTCTTGGAGG CCACAATGAT AGACAGAAAA ATAGGAGATA AACCCATCAG CTTTGAAGTC
    ACCATCGGTA ACTATGGTAA CCAGATTGAT GGTGTGAGCA AGCCTGCATT AGCGAAGAAG
    AAGAAGGAGG GTGGAGGAGA GAGTGAAGAA GAGGAGTCTG AGCTTATCCA CAACTCCAGC
    GAGGAAGAGG CAGAGGATGA TGGAGATCTG ACCTCTGTAC CGTCTACACC TCCTATGAAG
    CCTGTTATCA CCGACAGAAA TTACTTCCAC TTGCCTTACT TTGAAAAGAA ACCCTGCATT
    TACATCAAGA GCTGGTGGCA GGACCAGCGG AGACGTCTCT ACAACTCCAA CATAATGGAC
    AAGATAGCTG ATAAACTGGA GGAAGGTCTG AATGATGTTC AAGAGATCAT AAAAACAGAG
    AAGGCATATC CAGAACGAAG ACTAAGAGGT GTATTAGAAG AACTTAGCAC AAGCTGCAGC
    CGGTTTGTTA CACTTGCAAA CAAGGATCAG AATCTTTCTG GAAGAACCAA GCTGGACAGA
    GAGAGGCTGA AGTCCTGTAT GAGAGAAATG GAGAGTATGG GTCAGCAGGC GAAGACCATC
    CGCACACAAG TGAAAAGAAA CACAGTTCGA GATAAACTCA AGCTAGTGTT GAATTTCCTT
    CAGAGGCTTC GATTCTTAGC AGACGAGCCC CAGCACAGCA TCCCTGATGT GTTCATATGG
    ATGATTAGCA ACAACAAACG CATCGCATAC GCCCGCATAC CCTCCAAAGA CATCCTCTAC
    TCAATTGTCG ATGAAGAGAT GGGCAAAGAC TGTGGAAAAG TTAAAGCTGT TTTTCTCAGG
    CTGCCTGGAA AGAAAGGTTT TGGGCCTGCT GGCTGGACAG TTCAGGCTAA ATTAGAGATG
    TATTTGTGGC TGGGCCTGAA TAAACAGAGG AAAGACTTCC TGATTGGCCT CCCTAGTGGC
    TTTGAAGAAA ACAAGGCAGT AAAGGGAATT GGCATACAAG CTGTCCCTCC CATCAGCCTA
    GTTTATAACA TGAAGCAGGT GTTTCAGCTG AGGGCCCACA TGTATCAAGC TCGCAGTCTG
    TTTGCTGCGG ACACTAGTGG CCTGTCTGAT CCATTTGCAA GGGTTTTCTT CTCCACCCAC
    AGCCAGGTCA CGGAGGTCCT CAGTGAGACT CTCTGTCCAA CATGGGATCA GCTGCTTGTG
    TTTGATAATG TTGAACTTTA CGGTGAGGCC GGTGAACTCC GTGATGACCC TCCAATCATT
    GTCATTGAGT TGTATGATCA AGACACTGTG GGGAAAGCTG AGTTTATTGG CCGAACATTT
    GCAAAACCAC TTACTAAGAT GGTTGATGAA CACTACGGCC CACCGCGGTT TCCTCCCCAG
    CTCGAGTATT ATCAGATCTA CCGAGGAAAC TGTGCTGCGG GAGATCTATT GGCTGCTTTT
    GAGCTTCTAC AGATTGGTCC AGCAGGGCGG GCAGCACTTC CTCCAATCGA TGGACCGACC
    GATTCTGACC GTGGGCCCAT TCTGCCTGTT CCATTGGGAA TTCGACCAGT TCTCAGCAGA
    TATCGCATCG AGGTCCTGTT TTGGGGTCTG AGGGACCTTA AAAGGGTCAA CCTGGCTCAG
    GTGGACCGAC CTCGTGTGGA CATTGAGTGT GCAGGGAAAG GAGTTCAGTC AGCCCTAATT
    CAAAACTACA AGAAGAATCC AAACTTCAGC ACATTAGTAA AGTGGTTTGA AGTGGATCTG
    CCAGAAAATG AGCTGCTCCA TCCACCACTT AATATTCGGG TAGTGGATTG CAGGGCATTC
    GGACGCTACA TTTTGGTGGG GTCTCATGCT GTGACCACCC TTCGCAAGTT CATCTACAGC
    CCCCCAGACA AGACTGCTAA CAACTGGGCT CACACAGGTG ACATTGTGGT CAACATGAGC
    CCTGAACCTA ACATTAAGAA GATGGACACG GTTGTCAAGA TTGAAGCTAC CACCGATGCG
    GTTGTTAAAG TTGACTTGAA TGAGGATGAG AAGGAGAAAG AAAAGAAGAA GAAGAAGAAG
    AAGAAAGGAG AAGAAGTGGA GGAAGAGGAG CCGGATGAGA GCATGCTGGA CTGGTGGTCC
    AAATACTTTG CCTCAATAGA GACTATGATG GAGAATCTAA GAGCTCAGGA GGCCGCTCAG
    GCAGAGGCAG AGGAAAGAGA AGATTTGGAG ATTGCAGCAG AGAGTGCAGA GATCAAAGCT
    GATGACTTTC CTATGAAAGG CACCAAACCT AAAGAGAAGA GCAAAGACAA GAAGAGCACC
    AAGGACAAAA AGAAGAACAA CGATGGCACA GAAAAACGAC CTCCAAAACC AAAAGTTGAT
    GAACTCATGG TGTACAATAA AGAGCTGGAG AGTGAGTTCG GTAGCTTTGA GGACTGGCTT
    CACACCTTCA ACCTGTACCG AGGAAAGGCT GGAGATGATG ATGACCACAA TGTGGTTGAT
    GAGGACAGAA TTGTGGGCAG ATTCAAGGGC TCCCTCTGTA TGTATAAACT ACCACTGTCT
    GAGGAGATCA CCAGAGAGGC AGGATTTGAT CCAAACATGG GCATGTTCCA GAGTATTCCT
    CACAATGACC CTATCAACGT TCTTGTCAGG ATTTATATCA TTCGAGCCAC AGACCTGCAT
    CCTGCAGATA TAAATGGAAA GGCCGACCCT TATATAGTCA TTAAATTGGG AAAGTCAGAC
    ATTCGAGACA AGGAGAACTA CATATCCAAG CAGCTGAATC CTGTCTTTGG AAAGTCATTT
    GATATAGAGG CCACGTTCCC AATGGAGTCT ATGCTGACTG TGGCAGTGTA TGACTGGGAT
    TTAGTCGGAA CTGATGACCT GATTGGTGAG ACTAAGATTG ATTTGGAGAA CAGATACTAC
    AGCAAACACA GAGCCACATG TGGCATTGCT TCCAACTACT CTGTCCATGG TTATAATGTA
    TGGAGAGATC CTCAGAAACC AGCTCAGATC CTGGCTAAGT TGTGCAAAGA AGGTAAACTG
    GATGGACCTC ACTATGGACC TGGGGGAAGA GTCAAAGTTG CAAACCGGAT CTTTCTTGGG
    CCGACAGAAA TTGAGGATGA GAGTGGTCTG AAAAAACAAA CAGAAGAACA CTTGGCGCTC
    ACAGTCCTTA GACATTGGGA GGAAATCCCA CGTGTTGGTT GCAAACTCAT CCCTGAGCAT
    GTGGAGACGA GGCCTCTCCT CAACCCAGAC AAGCCAGGCA TAGAGCAGGG AAGGATTGAA
    ATGTGGGTGG ACATGTTTCC TATGGATGTC CCAGCACCAG GACCAGCCAT TGATATATCG
    CCACGCAAAC CAAAGAGATA TGAGCTCAGG GTGATAATAT GGAATACTGA CGAGGTAATT
    CTGGAAGACG ATGATTACTT CACAGGGGAA AAGTCCAGTG ACATTTTTGT GAGGGGCTGG
    CTTAAAGGAC AGCAGGAGGA CAAACAGGAC ACCGATGTAC ATTATCACTC TCTAACTGGA
    GAGGGAAACT TCAACTGGCG CTTTGTCTTC CCCTTTGACT ATCTCATGGC CGAGGAGAAG
    ATTGTTATCT CCAAGAAGGA ATCAATGTTT TCCTGGGATG AGACTGAGTA TAAGATCCCT
    GCTCGACTCA CCCTTCAAGT ATGGGATGCC GATCACTTTT CTGCAGATGA TTTTCTGGGT
    GCGATTGAGT TGGACTTGAA TAAGTTTCCG CGTGGGGCAA AAACAGCCAA GCAGTGCTCT
    CTAGACATGG TTCTCAAAGA GCATGAGCTG CCAACCATCT CCATCTTCAA ACAAAAGAGA
    GTCAAGGGCT GGTGGCCATT TGTGGCCCAA AATGAGAATG ATGAATTTGA GCTAACGGGA
    AAAGTAGAGG CAGAGCTCCA TCTGTTGACA GCAGAGGAGG CAGAGAAGAG TCCAGTCGGC
    CTGGGGAGGA ATGATCCGGA GCCACTTGAG AAACCAAATC GTCCAACTAC CAGCCTGTCG
    TGGCTGTTGA GTCCGATTCG GGTGACCAGC ATAATGTGTT GGCGGCAGTA TAAGTTGGTC
    ACAGTGGTGA CTCTGCTCAT CATAACCGGG CTGATCTTTT TGGGCCTCCT GTTCTTTTCT
    CTGCCTTCAG CTTTCATGCA GAAGCTCTTC AACATCTCTG GATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009293017.1
    CDS300..6284
    Translation

    Target ORF information:

    RefSeq Version XM_009294742.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio otoferlin a (otofa), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009294742.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGCTTTAG TGGTGCATCT GAAGACTGTC ACGGAGCTCC GTGGGAAGGG GGACCGAATC 
    GCTAAAGTCA CTTTCAGAGG TCTCTCCTTC TTCTCTCGGG TGTTGGAAAA CTGCGAGGAT
    GAAGCTCGAT TTGAGCAGGC GTTCCGATGG CCAATTGGGA GTCAAGTGGA TGGCGACGAG
    ATGCTGGAGA TCCAAGTGTT TAATTACAGC AAAGTCTTCA CTAATAGGTT AATCGGCACA
    TTCCGCATGG TTCTTCAGAA GGTGGTGGAG GAGGGACACC TGGAGGTGTC TGACACACTC
    ATTGACGACA ATAACACAGC CATACAGACA ACCATTTCCA TAGAGATCAA ATATCAGACC
    ATGGACGGGA GTGTAAAGGT GTGGAGCGAT GGTGAATTCC TTGATATTCC AGATGACCTT
    GATGGAACCT TCCAGTTTGA GACAGATAGT CTTCTCTCAG GACGCAGCCA GAGTTCTGGA
    ACTTCACCTG GACGATCCAT TCATGGCATT CCCACATTCC GCAAGGCAGG AAAAGGAGTG
    TTCTCAGCCA TGAAGCTGGG CAAGACCCGC ACCTCTAAAG ATGACCACAA AAAAGGAGAT
    GATGCTGCCA TTCTGGATGC AGAAGATCTG GATCGAAAAG CCATGCGTCT AGGCGGCATT
    CTTGACCCTG ATACAATCTC ACTGGCCTCG GTTACTGCAG TGACCACTAA TGTCTCCAAC
    AAAAGATCAA AGCCTGACAT TAAAATGGAG CCGAGTTCAG GGCGTCCGGT TGATTATCAG
    ATCAGTGTTA CAGTAATCGA GGCCCGTCAG CTGGTCGGAT TGAACATGGA CCCTGTGGTT
    TGCGTGGAAA TTGGAGAGGA GAAGAAGTAC ACGTCAATGA AGGAGTCAAC AAACTGCCCT
    TACTACAATG AGTATTTTGT CTTCGACTTC CACGTGCCTC CAGACGTCAT GTTTGACAAA
    ATTATAAAGA TTTCAGTAAT ACATTCTAAA AACCTTCTGC GAAGCGGCAC CCTCGTGGGC
    ACCTTCAAAC TAGACGTGGG GACTGTTTAC ACACAACCTG AACATCAGTT TCATCACAAA
    TGGGCGATGC TGTCAGACCC TGATGACATC ACGACTGGCT GCAAAGGTTA TGTTAAATGT
    GACATCGCAG TCGTGGGAAA AGGAGACAAC ATCAAGACTC CACACAAGGC CAGTGAAGCA
    GACGAGGATG ACATAGAGGG GAATCTTCTC TTGCCGGAGG GTGTTCCATC AGAGAGACAG
    TGGGCTCGGT TTTATGTTAA GATTTATAGA GCTGAGGGTC TGCCTAAAAT GAACACCAGT
    ATCATGGCCA ACGTGAAGAA AGCCTTTATT GGGGAAAACA GGGACCTTGT GGACCCTTAC
    GTCCTGGTGC AATTTGCAGG GCAAAAGGGC AAAACATCTG TTCAGAAGAG CAGCTACGAG
    CCCATCTGGA ACGAGCAAGT CATCTTCACG GAGATGTTCC CACCTTTGTG TAGGCGACTG
    AAAGTTCAGA TCCGTGATTC AGACAAGGTG AACGATGTTG CCATAGGAAC CCACTTCATT
    GATCTACGAA AGGTTTCCAA TGATGGAGAC AAAGGTTTCT TGCCCACTAT GGGTCCGGCT
    TGGGTGAATA TGTATGGGTC GACCCGTAAC TACACCCTGA TGGACGAACA CCAGGACCTG
    AATGAGGGGT TGGGGGAAGG GGTGTCCTTC AGAGCACGTC TGCTCATTAG TATCGCGGTG
    GAGATTCTAG ACACCACCTC TGCTGAGATC ATGAGCTCCA CTGAAGTACA CATGGAGCCG
    GTGTCCAACA TCTCAGAGAG TGCTACAGGA AAGATGGAAG AATTTTTCCT TTTCGGGTCA
    TTCTTGGAGG CCACAATGAT AGACAGAAAA ATAGGAGATA AACCCATCAG CTTTGAAGTC
    ACCATCGGTA ACTATGGTAA CCAGATTGAT GGTGTGAGCA AGCCTGCATT AGCGAAGAAG
    AAGAAGGAGG GTGGAGGAGA GAGTGAAGAA GAGGAGTCTG AGCTTATCCA CAACTCCAGC
    GAGGAAGAGG CAGAGGATGA TGGAGATCTG ACCTCTGTAC CGTCTACACC TCCTATGAAG
    CCTGTTATCA CCGACAGAAA TTACTTCCAC TTGCCTTACT TTGAAAAGAA ACCCTGCATT
    TACATCAAGA GCTGGTGGCA GGACCAGCGG AGACGTCTCT ACAACTCCAA CATAATGGAC
    AAGATAGCTG ATAAACTGGA GGAAGGTCTG AATGATGTTC AAGAGATCAT AAAAACAGAG
    AAGGCATATC CAGAACGAAG ACTAAGAGGT GTATTAGAAG AACTTAGCAC AAGCTGCAGC
    CGGTTTGTTA CACTTGCAAA CAAGGATCAG AATCTTTCTG GAAGAACCAA GCTGGACAGA
    GAGAGGCTGA AGTCCTGTAT GAGAGAAATG GAGAGTATGG GTCAGCAGGC GAAGACCATC
    CGCACACAAG TGAAAAGAAA CACAGTTCGA GATAAACTCA AGCTAGTGTT GAATTTCCTT
    CAGAGGCTTC GATTCTTAGC AGACGAGCCC CAGCACAGCA TCCCTGATGT GTTCATATGG
    ATGATTAGCA ACAACAAACG CATCGCATAC GCCCGCATAC CCTCCAAAGA CATCCTCTAC
    TCAATTGTCG ATGAAGAGAT GGGCAAAGAC TGTGGAAAAG TTAAAGCTGT TTTTCTCAGG
    CTGCCTGGAA AGAAAGGTTT TGGGCCTGCT GGCTGGACAG TTCAGGCTAA ATTAGAGATG
    TATTTGTGGC TGGGCCTGAA TAAACAGAGG AAAGACTTCC TGATTGGCCT CCCTAGTGGC
    TTTGAAGAAA ACAAGGCAGT AAAGGGAATT GGCATACAAG CTGTCCCTCC CATCAGCCTA
    GTTTATAACA TGAAGCAGGT GTTTCAGCTG AGGGCCCACA TGTATCAAGC TCGCAGTCTG
    TTTGCTGCGG ACACTAGTGG CCTGTCTGAT CCATTTGCAA GGGTTTTCTT CTCCACCCAC
    AGCCAGGTCA CGGAGGTCCT CAGTGAGACT CTCTGTCCAA CATGGGATCA GCTGCTTGTG
    TTTGATAATG TTGAACTTTA CGGTGAGGCC GGTGAACTCC GTGATGACCC TCCAATCATT
    GTCATTGAGT TGTATGATCA AGACACTGTG GGGAAAGCTG AGTTTATTGG CCGAACATTT
    GCAAAACCAC TTACTAAGAT GGTTGATGAA CACTACGGCC CACCGCGGTT TCCTCCCCAG
    CTCGAGTATT ATCAGATCTA CCGAGGAAAC TGTGCTGCGG GAGATCTATT GGCTGCTTTT
    GAGCTTCTAC AGATTGGTCC AGCAGGGCGG GCAGCACTTC CTCCAATCGA TGGACCGACC
    GATTCTGACC GTGGGCCCAT TCTGCCTGTT CCATTGGGAA TTCGACCAGT TCTCAGCAGA
    TATCGCATCG AGGTCCTGTT TTGGGGTCTG AGGGACCTTA AAAGGGTCAA CCTGGCTCAG
    GTGGACCGAC CTCGTGTGGA CATTGAGTGT GCAGGGAAAG GAGTTCAGTC AGCCCTAATT
    CAAAACTACA AGAAGAATCC AAACTTCAGC ACATTAGTAA AGTGGTTTGA AGTGGATCTG
    CCAGAAAATG AGCTGCTCCA TCCACCACTT AATATTCGGG TAGTGGATTG CAGGGCATTC
    GGACGCTACA TTTTGGTGGG GTCTCATGCT GTGACCACCC TTCGCAAGTT CATCTACAGC
    CCCCCAGACA AGACTGCTAA CAACTGGGCT CACACAGGTG ACATTGTGGT CAACATGAGC
    CCTGAACCTA ACATTAAGAA GATGGACACG GTTGTCAAGA TTGAAGCTAC CACCGATGCG
    GTTGTTAAAG TTGACTTGAA TGAGGATGAG AAGGAGAAAG AAAAGAAGAA GAAGAAGAAG
    AAGAAAGGAG AAGAAGTGGA GGAAGAGGAG CCGGATGAGA GCATGCTGGA CTGGTGGTCC
    AAATACTTTG CCTCAATAGA GACTATGATG GAGAATCTAA GAGCTCAGGA GGCCGCTCAG
    GCAGAGGCAG AGGAAAGAGA AGATTTGGAG ATTGCAGCAG AGAGTGCAGA GATCAAAGCT
    GATGACTTTC CTATGAAAGG CACCAAACCT AAAGAGAAGA GCAAAGACAA GAAGAGCACC
    AAGGACAAAA AGAAGAACAA CGATGGCACA GAAAAACGAC CTCCAAAACC AAAAGTTGAT
    GAACTCATGG TGTACAATAA AGAGCTGGAG AGTGAGTTCG GTAGCTTTGA GGACTGGCTT
    CACACCTTCA ACCTGTACCG AGGAAAGGCT GGAGATGATG ATGACCACAA TGTGGTTGAT
    GAGGACAGAA TTGTGGGCAG ATTCAAGGGC TCCCTCTGTA TGTATAAACT ACCACTGTCT
    GAGGAGATCA CCAGAGAGGC AGGATTTGAT CCAAACATGG GCATGTTCCA GAGTATTCCT
    CACAATGACC CTATCAACGT TCTTGTCAGG ATTTATATCA TTCGAGCCAC AGACCTGCAT
    CCTGCAGATA TAAATGGAAA GGCCGACCCT TATATAGTCA TTAAATTGGG AAAGTCAGAC
    ATTCGAGACA AGGAGAACTA CATATCCAAG CAGCTGAATC CTGTCTTTGG AAAGTCATTT
    GATATAGAGG CCACGTTCCC AATGGAGTCT ATGCTGACTG TGGCAGTGTA TGACTGGGAT
    TTAGTCGGAA CTGATGACCT GATTGGTGAG ACTAAGATTG ATTTGGAGAA CAGATACTAC
    AGCAAACACA GAGCCACATG TGGCATTGCT TCCAACTACT CTGTCCATGG TTATAATGTA
    TGGAGAGATC CTCAGAAACC AGCTCAGATC CTGGCTAAGT TGTGCAAAGA AGGTAAACTG
    GATGGACCTC ACTATGGACC TGGGGGAAGA GTCAAAGTTG CAAACCGGAT CTTTCTTGGG
    CCGACAGAAA TTGAGGATGA GAGTGGTCTG AAAAAACAAA CAGAAGAACA CTTGGCGCTC
    ACAGTCCTTA GACATTGGGA GGAAATCCCA CGTGTTGGTT GCAAACTCAT CCCTGAGCAT
    GTGGAGACGA GGCCTCTCCT CAACCCAGAC AAGCCAGGCA TAGAGCAGGG AAGGATTGAA
    ATGTGGGTGG ACATGTTTCC TATGGATGTC CCAGCACCAG GACCAGCCAT TGATATATCG
    CCACGCAAAC CAAAGAGATA TGAGCTCAGG GTGATAATAT GGAATACTGA CGAGGTAATT
    CTGGAAGACG ATGATTACTT CACAGGGGAA AAGTCCAGTG ACATTTTTGT GAGGGGCTGG
    CTTAAAGGAC AGCAGGAGGA CAAACAGGAC ACCGATGTAC ATTATCACTC TCTAACTGGA
    GAGGGAAACT TCAACTGGCG CTTTGTCTTC CCCTTTGACT ATCTCATGGC CGAGGAGAAG
    ATTGTTATCT CCAAGAAGGA ATCAATGTTT TCCTGGGATG AGACTGAGTA TAAGATCCCT
    GCTCGACTCA CCCTTCAAGT ATGGGATGCC GATCACTTTT CTGCAGATGA TTTTCTGGGT
    GCGATTGAGT TGGACTTGAA TAAGTTTCCG CGTGGGGCAA AAACAGCCAA GCAGTGCTCT
    CTAGACATGG TTCTCAAAGA GCATGAGCTG CCAACCATCT CCATCTTCAA ACAAAAGAGA
    GTCAAGGGCT GGTGGCCATT TGTGGCCCAA AATGAGAATG ATGAATTTGA GCTAACGGGA
    AAAGTAGAGG CAGAGCTCCA TCTGTTGACA GCAGAGGAGG CAGAGAAGAG TCCAGTCGGC
    CTGGGGAGGA ATGATCCGGA GCCACTTGAG AAACCAAATC GTCCAACTAC CAGCCTGTCG
    TGGCTGTTGA GTCCGATTCG GGTGACCAGC ATAATGTGTT GGCGGCAGTA TAAGTTGGTC
    ACAGTGGTGA CTCTGCTCAT CATAACCGGG CTGATCTTTT TGGGCCTCCT GTTCTTTTCT
    CTGCCTTCAG CTTTCATGCA GAAGCTCTTC AACATCTCTG GATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42613
    Clone ID Related Accession (Same CDS sequence) XM_009294742.3 , XM_009294742.2
    Accession Version XM_009294742.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5985bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product otoferlin isoform X8
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009294742.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGCTTTAG TGGTGCATCT GAAGACTGTC ACGGAGCTCC GTGGGAAGGG GGACCGAATC 
    GCTAAAGTCA CTTTCAGAGG TCTCTCCTTC TTCTCTCGGG TGTTGGAAAA CTGCGAGGAT
    GAAGCTCGAT TTGAGCAGGC GTTCCGATGG CCAATTGGGA GTCAAGTGGA TGGCGACGAG
    ATGCTGGAGA TCCAAGTGTT TAATTACAGC AAAGTCTTCA CTAATAGGTT AATCGGCACA
    TTCCGCATGG TTCTTCAGAA GGTGGTGGAG GAGGGACACC TGGAGGTGTC TGACACACTC
    ATTGACGACA ATAACACAGC CATACAGACA ACCATTTCCA TAGAGATCAA ATATCAGACC
    ATGGACGGGA GTGTAAAGGT GTGGAGCGAT GGTGAATTCC TTGATATTCC AGATGACCTT
    GATGGAACCT TCCAGTTTGA GACAGATAGT CTTCTCTCAG GACGCAGCCA GAGTTCTGGA
    ACTTCACCTG GACGATCCAT TCATGGCATT CCCACATTCC GCAAGGCAGG AAAAGGAGTG
    TTCTCAGCCA TGAAGCTGGG CAAGACCCGC ACCTCTAAAG ATGACCACAA AAAAGGAGAT
    GATGCTGCCA TTCTGGATGC AGAAGATCTG GATCGAAAAG CCATGCGTCT AGGCGGCATT
    CTTGACCCTG ATACAATCTC ACTGGCCTCG GTTACTGCAG TGACCACTAA TGTCTCCAAC
    AAAAGATCAA AGCCTGACAT TAAAATGGAG CCGAGTTCAG GGCGTCCGGT TGATTATCAG
    ATCAGTGTTA CAGTAATCGA GGCCCGTCAG CTGGTCGGAT TGAACATGGA CCCTGTGGTT
    TGCGTGGAAA TTGGAGAGGA GAAGAAGTAC ACGTCAATGA AGGAGTCAAC AAACTGCCCT
    TACTACAATG AGTATTTTGT CTTCGACTTC CACGTGCCTC CAGACGTCAT GTTTGACAAA
    ATTATAAAGA TTTCAGTAAT ACATTCTAAA AACCTTCTGC GAAGCGGCAC CCTCGTGGGC
    ACCTTCAAAC TAGACGTGGG GACTGTTTAC ACACAACCTG AACATCAGTT TCATCACAAA
    TGGGCGATGC TGTCAGACCC TGATGACATC ACGACTGGCT GCAAAGGTTA TGTTAAATGT
    GACATCGCAG TCGTGGGAAA AGGAGACAAC ATCAAGACTC CACACAAGGC CAGTGAAGCA
    GACGAGGATG ACATAGAGGG GAATCTTCTC TTGCCGGAGG GTGTTCCATC AGAGAGACAG
    TGGGCTCGGT TTTATGTTAA GATTTATAGA GCTGAGGGTC TGCCTAAAAT GAACACCAGT
    ATCATGGCCA ACGTGAAGAA AGCCTTTATT GGGGAAAACA GGGACCTTGT GGACCCTTAC
    GTCCTGGTGC AATTTGCAGG GCAAAAGGGC AAAACATCTG TTCAGAAGAG CAGCTACGAG
    CCCATCTGGA ACGAGCAAGT CATCTTCACG GAGATGTTCC CACCTTTGTG TAGGCGACTG
    AAAGTTCAGA TCCGTGATTC AGACAAGGTG AACGATGTTG CCATAGGAAC CCACTTCATT
    GATCTACGAA AGGTTTCCAA TGATGGAGAC AAAGGTTTCT TGCCCACTAT GGGTCCGGCT
    TGGGTGAATA TGTATGGGTC GACCCGTAAC TACACCCTGA TGGACGAACA CCAGGACCTG
    AATGAGGGGT TGGGGGAAGG GGTGTCCTTC AGAGCACGTC TGCTCATTAG TATCGCGGTG
    GAGATTCTAG ACACCACCTC TGCTGAGATC ATGAGCTCCA CTGAAGTACA CATGGAGCCG
    GTGTCCAACA TCTCAGAGAG TGCTACAGGA AAGATGGAAG AATTTTTCCT TTTCGGGTCA
    TTCTTGGAGG CCACAATGAT AGACAGAAAA ATAGGAGATA AACCCATCAG CTTTGAAGTC
    ACCATCGGTA ACTATGGTAA CCAGATTGAT GGTGTGAGCA AGCCTGCATT AGCGAAGAAG
    AAGAAGGAGG GTGGAGGAGA GAGTGAAGAA GAGGAGTCTG AGCTTATCCA CAACTCCAGC
    GAGGAAGAGG CAGAGGATGA TGGAGATCTG ACCTCTGTAC CGTCTACACC TCCTATGAAG
    CCTGTTATCA CCGACAGAAA TTACTTCCAC TTGCCTTACT TTGAAAAGAA ACCCTGCATT
    TACATCAAGA GCTGGTGGCA GGACCAGCGG AGACGTCTCT ACAACTCCAA CATAATGGAC
    AAGATAGCTG ATAAACTGGA GGAAGGTCTG AATGATGTTC AAGAGATCAT AAAAACAGAG
    AAGGCATATC CAGAACGAAG ACTAAGAGGT GTATTAGAAG AACTTAGCAC AAGCTGCAGC
    CGGTTTGTTA CACTTGCAAA CAAGGATCAG AATCTTTCTG GAAGAACCAA GCTGGACAGA
    GAGAGGCTGA AGTCCTGTAT GAGAGAAATG GAGAGTATGG GTCAGCAGGC GAAGACCATC
    CGCACACAAG TGAAAAGAAA CACAGTTCGA GATAAACTCA AGCTAGTGTT GAATTTCCTT
    CAGAGGCTTC GATTCTTAGC AGACGAGCCC CAGCACAGCA TCCCTGATGT GTTCATATGG
    ATGATTAGCA ACAACAAACG CATCGCATAC GCCCGCATAC CCTCCAAAGA CATCCTCTAC
    TCAATTGTCG ATGAAGAGAT GGGCAAAGAC TGTGGAAAAG TTAAAGCTGT TTTTCTCAGG
    CTGCCTGGAA AGAAAGGTTT TGGGCCTGCT GGCTGGACAG TTCAGGCTAA ATTAGAGATG
    TATTTGTGGC TGGGCCTGAA TAAACAGAGG AAAGACTTCC TGATTGGCCT CCCTAGTGGC
    TTTGAAGAAA ACAAGGCAGT AAAGGGAATT GGCATACAAG CTGTCCCTCC CATCAGCCTA
    GTTTATAACA TGAAGCAGGT GTTTCAGCTG AGGGCCCACA TGTATCAAGC TCGCAGTCTG
    TTTGCTGCGG ACACTAGTGG CCTGTCTGAT CCATTTGCAA GGGTTTTCTT CTCCACCCAC
    AGCCAGGTCA CGGAGGTCCT CAGTGAGACT CTCTGTCCAA CATGGGATCA GCTGCTTGTG
    TTTGATAATG TTGAACTTTA CGGTGAGGCC GGTGAACTCC GTGATGACCC TCCAATCATT
    GTCATTGAGT TGTATGATCA AGACACTGTG GGGAAAGCTG AGTTTATTGG CCGAACATTT
    GCAAAACCAC TTACTAAGAT GGTTGATGAA CACTACGGCC CACCGCGGTT TCCTCCCCAG
    CTCGAGTATT ATCAGATCTA CCGAGGAAAC TGTGCTGCGG GAGATCTATT GGCTGCTTTT
    GAGCTTCTAC AGATTGGTCC AGCAGGGCGG GCAGCACTTC CTCCAATCGA TGGACCGACC
    GATTCTGACC GTGGGCCCAT TCTGCCTGTT CCATTGGGAA TTCGACCAGT TCTCAGCAGA
    TATCGCATCG AGGTCCTGTT TTGGGGTCTG AGGGACCTTA AAAGGGTCAA CCTGGCTCAG
    GTGGACCGAC CTCGTGTGGA CATTGAGTGT GCAGGGAAAG GAGTTCAGTC AGCCCTAATT
    CAAAACTACA AGAAGAATCC AAACTTCAGC ACATTAGTAA AGTGGTTTGA AGTGGATCTG
    CCAGAAAATG AGCTGCTCCA TCCACCACTT AATATTCGGG TAGTGGATTG CAGGGCATTC
    GGACGCTACA TTTTGGTGGG GTCTCATGCT GTGACCACCC TTCGCAAGTT CATCTACAGC
    CCCCCAGACA AGACTGCTAA CAACTGGGCT CACACAGGTG ACATTGTGGT CAACATGAGC
    CCTGAACCTA ACATTAAGAA GATGGACACG GTTGTCAAGA TTGAAGCTAC CACCGATGCG
    GTTGTTAAAG TTGACTTGAA TGAGGATGAG AAGGAGAAAG AAAAGAAGAA GAAGAAGAAG
    AAGAAAGGAG AAGAAGTGGA GGAAGAGGAG CCGGATGAGA GCATGCTGGA CTGGTGGTCC
    AAATACTTTG CCTCAATAGA GACTATGATG GAGAATCTAA GAGCTCAGGA GGCCGCTCAG
    GCAGAGGCAG AGGAAAGAGA AGATTTGGAG ATTGCAGCAG AGAGTGCAGA GATCAAAGCT
    GATGACTTTC CTATGAAAGG CACCAAACCT AAAGAGAAGA GCAAAGACAA GAAGAGCACC
    AAGGACAAAA AGAAGAACAA CGATGGCACA GAAAAACGAC CTCCAAAACC AAAAGTTGAT
    GAACTCATGG TGTACAATAA AGAGCTGGAG AGTGAGTTCG GTAGCTTTGA GGACTGGCTT
    CACACCTTCA ACCTGTACCG AGGAAAGGCT GGAGATGATG ATGACCACAA TGTGGTTGAT
    GAGGACAGAA TTGTGGGCAG ATTCAAGGGC TCCCTCTGTA TGTATAAACT ACCACTGTCT
    GAGGAGATCA CCAGAGAGGC AGGATTTGAT CCAAACATGG GCATGTTCCA GAGTATTCCT
    CACAATGACC CTATCAACGT TCTTGTCAGG ATTTATATCA TTCGAGCCAC AGACCTGCAT
    CCTGCAGATA TAAATGGAAA GGCCGACCCT TATATAGTCA TTAAATTGGG AAAGTCAGAC
    ATTCGAGACA AGGAGAACTA CATATCCAAG CAGCTGAATC CTGTCTTTGG AAAGTCATTT
    GATATAGAGG CCACGTTCCC AATGGAGTCT ATGCTGACTG TGGCAGTGTA TGACTGGGAT
    TTAGTCGGAA CTGATGACCT GATTGGTGAG ACTAAGATTG ATTTGGAGAA CAGATACTAC
    AGCAAACACA GAGCCACATG TGGCATTGCT TCCAACTACT CTGTCCATGG TTATAATGTA
    TGGAGAGATC CTCAGAAACC AGCTCAGATC CTGGCTAAGT TGTGCAAAGA AGGTAAACTG
    GATGGACCTC ACTATGGACC TGGGGGAAGA GTCAAAGTTG CAAACCGGAT CTTTCTTGGG
    CCGACAGAAA TTGAGGATGA GAGTGGTCTG AAAAAACAAA CAGAAGAACA CTTGGCGCTC
    ACAGTCCTTA GACATTGGGA GGAAATCCCA CGTGTTGGTT GCAAACTCAT CCCTGAGCAT
    GTGGAGACGA GGCCTCTCCT CAACCCAGAC AAGCCAGGCA TAGAGCAGGG AAGGATTGAA
    ATGTGGGTGG ACATGTTTCC TATGGATGTC CCAGCACCAG GACCAGCCAT TGATATATCG
    CCACGCAAAC CAAAGAGATA TGAGCTCAGG GTGATAATAT GGAATACTGA CGAGGTAATT
    CTGGAAGACG ATGATTACTT CACAGGGGAA AAGTCCAGTG ACATTTTTGT GAGGGGCTGG
    CTTAAAGGAC AGCAGGAGGA CAAACAGGAC ACCGATGTAC ATTATCACTC TCTAACTGGA
    GAGGGAAACT TCAACTGGCG CTTTGTCTTC CCCTTTGACT ATCTCATGGC CGAGGAGAAG
    ATTGTTATCT CCAAGAAGGA ATCAATGTTT TCCTGGGATG AGACTGAGTA TAAGATCCCT
    GCTCGACTCA CCCTTCAAGT ATGGGATGCC GATCACTTTT CTGCAGATGA TTTTCTGGGT
    GCGATTGAGT TGGACTTGAA TAAGTTTCCG CGTGGGGCAA AAACAGCCAA GCAGTGCTCT
    CTAGACATGG TTCTCAAAGA GCATGAGCTG CCAACCATCT CCATCTTCAA ACAAAAGAGA
    GTCAAGGGCT GGTGGCCATT TGTGGCCCAA AATGAGAATG ATGAATTTGA GCTAACGGGA
    AAAGTAGAGG CAGAGCTCCA TCTGTTGACA GCAGAGGAGG CAGAGAAGAG TCCAGTCGGC
    CTGGGGAGGA ATGATCCGGA GCCACTTGAG AAACCAAATC GTCCAACTAC CAGCCTGTCG
    TGGCTGTTGA GTCCGATTCG GGTGACCAGC ATAATGTGTT GGCGGCAGTA TAAGTTGGTC
    ACAGTGGTGA CTCTGCTCAT CATAACCGGG CTGATCTTTT TGGGCCTCCT GTTCTTTTCT
    CTGCCTTCAG CTTTCATGCA GAAGCTCTTC AACATCTCTG GATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009293017.1
    CDS303..6287
    Translation

    Target ORF information:

    RefSeq Version XM_009294742.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio otoferlin a (otofa), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009294742.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGCTTTAG TGGTGCATCT GAAGACTGTC ACGGAGCTCC GTGGGAAGGG GGACCGAATC 
    GCTAAAGTCA CTTTCAGAGG TCTCTCCTTC TTCTCTCGGG TGTTGGAAAA CTGCGAGGAT
    GAAGCTCGAT TTGAGCAGGC GTTCCGATGG CCAATTGGGA GTCAAGTGGA TGGCGACGAG
    ATGCTGGAGA TCCAAGTGTT TAATTACAGC AAAGTCTTCA CTAATAGGTT AATCGGCACA
    TTCCGCATGG TTCTTCAGAA GGTGGTGGAG GAGGGACACC TGGAGGTGTC TGACACACTC
    ATTGACGACA ATAACACAGC CATACAGACA ACCATTTCCA TAGAGATCAA ATATCAGACC
    ATGGACGGGA GTGTAAAGGT GTGGAGCGAT GGTGAATTCC TTGATATTCC AGATGACCTT
    GATGGAACCT TCCAGTTTGA GACAGATAGT CTTCTCTCAG GACGCAGCCA GAGTTCTGGA
    ACTTCACCTG GACGATCCAT TCATGGCATT CCCACATTCC GCAAGGCAGG AAAAGGAGTG
    TTCTCAGCCA TGAAGCTGGG CAAGACCCGC ACCTCTAAAG ATGACCACAA AAAAGGAGAT
    GATGCTGCCA TTCTGGATGC AGAAGATCTG GATCGAAAAG CCATGCGTCT AGGCGGCATT
    CTTGACCCTG ATACAATCTC ACTGGCCTCG GTTACTGCAG TGACCACTAA TGTCTCCAAC
    AAAAGATCAA AGCCTGACAT TAAAATGGAG CCGAGTTCAG GGCGTCCGGT TGATTATCAG
    ATCAGTGTTA CAGTAATCGA GGCCCGTCAG CTGGTCGGAT TGAACATGGA CCCTGTGGTT
    TGCGTGGAAA TTGGAGAGGA GAAGAAGTAC ACGTCAATGA AGGAGTCAAC AAACTGCCCT
    TACTACAATG AGTATTTTGT CTTCGACTTC CACGTGCCTC CAGACGTCAT GTTTGACAAA
    ATTATAAAGA TTTCAGTAAT ACATTCTAAA AACCTTCTGC GAAGCGGCAC CCTCGTGGGC
    ACCTTCAAAC TAGACGTGGG GACTGTTTAC ACACAACCTG AACATCAGTT TCATCACAAA
    TGGGCGATGC TGTCAGACCC TGATGACATC ACGACTGGCT GCAAAGGTTA TGTTAAATGT
    GACATCGCAG TCGTGGGAAA AGGAGACAAC ATCAAGACTC CACACAAGGC CAGTGAAGCA
    GACGAGGATG ACATAGAGGG GAATCTTCTC TTGCCGGAGG GTGTTCCATC AGAGAGACAG
    TGGGCTCGGT TTTATGTTAA GATTTATAGA GCTGAGGGTC TGCCTAAAAT GAACACCAGT
    ATCATGGCCA ACGTGAAGAA AGCCTTTATT GGGGAAAACA GGGACCTTGT GGACCCTTAC
    GTCCTGGTGC AATTTGCAGG GCAAAAGGGC AAAACATCTG TTCAGAAGAG CAGCTACGAG
    CCCATCTGGA ACGAGCAAGT CATCTTCACG GAGATGTTCC CACCTTTGTG TAGGCGACTG
    AAAGTTCAGA TCCGTGATTC AGACAAGGTG AACGATGTTG CCATAGGAAC CCACTTCATT
    GATCTACGAA AGGTTTCCAA TGATGGAGAC AAAGGTTTCT TGCCCACTAT GGGTCCGGCT
    TGGGTGAATA TGTATGGGTC GACCCGTAAC TACACCCTGA TGGACGAACA CCAGGACCTG
    AATGAGGGGT TGGGGGAAGG GGTGTCCTTC AGAGCACGTC TGCTCATTAG TATCGCGGTG
    GAGATTCTAG ACACCACCTC TGCTGAGATC ATGAGCTCCA CTGAAGTACA CATGGAGCCG
    GTGTCCAACA TCTCAGAGAG TGCTACAGGA AAGATGGAAG AATTTTTCCT TTTCGGGTCA
    TTCTTGGAGG CCACAATGAT AGACAGAAAA ATAGGAGATA AACCCATCAG CTTTGAAGTC
    ACCATCGGTA ACTATGGTAA CCAGATTGAT GGTGTGAGCA AGCCTGCATT AGCGAAGAAG
    AAGAAGGAGG GTGGAGGAGA GAGTGAAGAA GAGGAGTCTG AGCTTATCCA CAACTCCAGC
    GAGGAAGAGG CAGAGGATGA TGGAGATCTG ACCTCTGTAC CGTCTACACC TCCTATGAAG
    CCTGTTATCA CCGACAGAAA TTACTTCCAC TTGCCTTACT TTGAAAAGAA ACCCTGCATT
    TACATCAAGA GCTGGTGGCA GGACCAGCGG AGACGTCTCT ACAACTCCAA CATAATGGAC
    AAGATAGCTG ATAAACTGGA GGAAGGTCTG AATGATGTTC AAGAGATCAT AAAAACAGAG
    AAGGCATATC CAGAACGAAG ACTAAGAGGT GTATTAGAAG AACTTAGCAC AAGCTGCAGC
    CGGTTTGTTA CACTTGCAAA CAAGGATCAG AATCTTTCTG GAAGAACCAA GCTGGACAGA
    GAGAGGCTGA AGTCCTGTAT GAGAGAAATG GAGAGTATGG GTCAGCAGGC GAAGACCATC
    CGCACACAAG TGAAAAGAAA CACAGTTCGA GATAAACTCA AGCTAGTGTT GAATTTCCTT
    CAGAGGCTTC GATTCTTAGC AGACGAGCCC CAGCACAGCA TCCCTGATGT GTTCATATGG
    ATGATTAGCA ACAACAAACG CATCGCATAC GCCCGCATAC CCTCCAAAGA CATCCTCTAC
    TCAATTGTCG ATGAAGAGAT GGGCAAAGAC TGTGGAAAAG TTAAAGCTGT TTTTCTCAGG
    CTGCCTGGAA AGAAAGGTTT TGGGCCTGCT GGCTGGACAG TTCAGGCTAA ATTAGAGATG
    TATTTGTGGC TGGGCCTGAA TAAACAGAGG AAAGACTTCC TGATTGGCCT CCCTAGTGGC
    TTTGAAGAAA ACAAGGCAGT AAAGGGAATT GGCATACAAG CTGTCCCTCC CATCAGCCTA
    GTTTATAACA TGAAGCAGGT GTTTCAGCTG AGGGCCCACA TGTATCAAGC TCGCAGTCTG
    TTTGCTGCGG ACACTAGTGG CCTGTCTGAT CCATTTGCAA GGGTTTTCTT CTCCACCCAC
    AGCCAGGTCA CGGAGGTCCT CAGTGAGACT CTCTGTCCAA CATGGGATCA GCTGCTTGTG
    TTTGATAATG TTGAACTTTA CGGTGAGGCC GGTGAACTCC GTGATGACCC TCCAATCATT
    GTCATTGAGT TGTATGATCA AGACACTGTG GGGAAAGCTG AGTTTATTGG CCGAACATTT
    GCAAAACCAC TTACTAAGAT GGTTGATGAA CACTACGGCC CACCGCGGTT TCCTCCCCAG
    CTCGAGTATT ATCAGATCTA CCGAGGAAAC TGTGCTGCGG GAGATCTATT GGCTGCTTTT
    GAGCTTCTAC AGATTGGTCC AGCAGGGCGG GCAGCACTTC CTCCAATCGA TGGACCGACC
    GATTCTGACC GTGGGCCCAT TCTGCCTGTT CCATTGGGAA TTCGACCAGT TCTCAGCAGA
    TATCGCATCG AGGTCCTGTT TTGGGGTCTG AGGGACCTTA AAAGGGTCAA CCTGGCTCAG
    GTGGACCGAC CTCGTGTGGA CATTGAGTGT GCAGGGAAAG GAGTTCAGTC AGCCCTAATT
    CAAAACTACA AGAAGAATCC AAACTTCAGC ACATTAGTAA AGTGGTTTGA AGTGGATCTG
    CCAGAAAATG AGCTGCTCCA TCCACCACTT AATATTCGGG TAGTGGATTG CAGGGCATTC
    GGACGCTACA TTTTGGTGGG GTCTCATGCT GTGACCACCC TTCGCAAGTT CATCTACAGC
    CCCCCAGACA AGACTGCTAA CAACTGGGCT CACACAGGTG ACATTGTGGT CAACATGAGC
    CCTGAACCTA ACATTAAGAA GATGGACACG GTTGTCAAGA TTGAAGCTAC CACCGATGCG
    GTTGTTAAAG TTGACTTGAA TGAGGATGAG AAGGAGAAAG AAAAGAAGAA GAAGAAGAAG
    AAGAAAGGAG AAGAAGTGGA GGAAGAGGAG CCGGATGAGA GCATGCTGGA CTGGTGGTCC
    AAATACTTTG CCTCAATAGA GACTATGATG GAGAATCTAA GAGCTCAGGA GGCCGCTCAG
    GCAGAGGCAG AGGAAAGAGA AGATTTGGAG ATTGCAGCAG AGAGTGCAGA GATCAAAGCT
    GATGACTTTC CTATGAAAGG CACCAAACCT AAAGAGAAGA GCAAAGACAA GAAGAGCACC
    AAGGACAAAA AGAAGAACAA CGATGGCACA GAAAAACGAC CTCCAAAACC AAAAGTTGAT
    GAACTCATGG TGTACAATAA AGAGCTGGAG AGTGAGTTCG GTAGCTTTGA GGACTGGCTT
    CACACCTTCA ACCTGTACCG AGGAAAGGCT GGAGATGATG ATGACCACAA TGTGGTTGAT
    GAGGACAGAA TTGTGGGCAG ATTCAAGGGC TCCCTCTGTA TGTATAAACT ACCACTGTCT
    GAGGAGATCA CCAGAGAGGC AGGATTTGAT CCAAACATGG GCATGTTCCA GAGTATTCCT
    CACAATGACC CTATCAACGT TCTTGTCAGG ATTTATATCA TTCGAGCCAC AGACCTGCAT
    CCTGCAGATA TAAATGGAAA GGCCGACCCT TATATAGTCA TTAAATTGGG AAAGTCAGAC
    ATTCGAGACA AGGAGAACTA CATATCCAAG CAGCTGAATC CTGTCTTTGG AAAGTCATTT
    GATATAGAGG CCACGTTCCC AATGGAGTCT ATGCTGACTG TGGCAGTGTA TGACTGGGAT
    TTAGTCGGAA CTGATGACCT GATTGGTGAG ACTAAGATTG ATTTGGAGAA CAGATACTAC
    AGCAAACACA GAGCCACATG TGGCATTGCT TCCAACTACT CTGTCCATGG TTATAATGTA
    TGGAGAGATC CTCAGAAACC AGCTCAGATC CTGGCTAAGT TGTGCAAAGA AGGTAAACTG
    GATGGACCTC ACTATGGACC TGGGGGAAGA GTCAAAGTTG CAAACCGGAT CTTTCTTGGG
    CCGACAGAAA TTGAGGATGA GAGTGGTCTG AAAAAACAAA CAGAAGAACA CTTGGCGCTC
    ACAGTCCTTA GACATTGGGA GGAAATCCCA CGTGTTGGTT GCAAACTCAT CCCTGAGCAT
    GTGGAGACGA GGCCTCTCCT CAACCCAGAC AAGCCAGGCA TAGAGCAGGG AAGGATTGAA
    ATGTGGGTGG ACATGTTTCC TATGGATGTC CCAGCACCAG GACCAGCCAT TGATATATCG
    CCACGCAAAC CAAAGAGATA TGAGCTCAGG GTGATAATAT GGAATACTGA CGAGGTAATT
    CTGGAAGACG ATGATTACTT CACAGGGGAA AAGTCCAGTG ACATTTTTGT GAGGGGCTGG
    CTTAAAGGAC AGCAGGAGGA CAAACAGGAC ACCGATGTAC ATTATCACTC TCTAACTGGA
    GAGGGAAACT TCAACTGGCG CTTTGTCTTC CCCTTTGACT ATCTCATGGC CGAGGAGAAG
    ATTGTTATCT CCAAGAAGGA ATCAATGTTT TCCTGGGATG AGACTGAGTA TAAGATCCCT
    GCTCGACTCA CCCTTCAAGT ATGGGATGCC GATCACTTTT CTGCAGATGA TTTTCTGGGT
    GCGATTGAGT TGGACTTGAA TAAGTTTCCG CGTGGGGCAA AAACAGCCAA GCAGTGCTCT
    CTAGACATGG TTCTCAAAGA GCATGAGCTG CCAACCATCT CCATCTTCAA ACAAAAGAGA
    GTCAAGGGCT GGTGGCCATT TGTGGCCCAA AATGAGAATG ATGAATTTGA GCTAACGGGA
    AAAGTAGAGG CAGAGCTCCA TCTGTTGACA GCAGAGGAGG CAGAGAAGAG TCCAGTCGGC
    CTGGGGAGGA ATGATCCGGA GCCACTTGAG AAACCAAATC GTCCAACTAC CAGCCTGTCG
    TGGCTGTTGA GTCCGATTCG GGTGACCAGC ATAATGTGTT GGCGGCAGTA TAAGTTGGTC
    ACAGTGGTGA CTCTGCTCAT CATAACCGGG CTGATCTTTT TGGGCCTCCT GTTCTTTTCT
    CTGCCTTCAG CTTTCATGCA GAAGCTCTTC AACATCTCTG GATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42614
    Clone ID Related Accession (Same CDS sequence) XM_021468443.1 , XM_009294743.2
    Accession Version XM_021468443.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6063bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product otoferlin isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGGCTTTAG TGGTGCATCT GAAGACTGTC ACGGAGCTCC GTGGGAAGGG GGACCGAATC 
    GCTAAAGTCA CTTTCAGAGG TCTCTCCTTC TTCTCTCGGG TGTTGGAAAA CTGCGAGGAT
    GAAGCTCGAT TTGAGCAGGC GTTCCGATGG CCAATTGGGA GTCAAGTGGA TGGCGACGAG
    ATGCTGGAGA TCCAAGTGTT TAATTACAGC AAAGTCTTCA CTAATAGGTT AATCGGCACA
    TTCCGCATGG TTCTTCAGAA GGTGGTGGAG GAGGGACACC TGGAGGTGTC TGACACACTC
    ATTGACGACA ATAACACAGC CATACAGACA ACCATTTCCA TAGAGATCAA ATATCAGACC
    ATGGACGGGA GTGTAAAGGT GTGGAGCGAT GGTGAATTCC TTGATATTCC AGATGACCTT
    GATGGAACCT TCCAGTTTGA GACAGATAGT CTTCTCTCAG GACGCAGCCA GAGTTCTGGA
    ACTTCACCTG GACGATCCAT TCATGGCATT CCCACATTCC GCAAGGCAGG AAAAGGAGTG
    TTCTCAGCCA TGAAGCTGGG CAAGACCCGC ACCTCTAAAG ATGACCACAA AAAAGGAGAT
    GATGCTGCCA TTCTGGATGC AGAAGATCTG GATCGAAAAG CCATGCGTCT AGGCGGCATT
    CTTGACCCTG ATACAATCTC ACTGGCCTCG GTTACTGCAG TGACCACTAA TGTCTCCAAC
    AAAAGATCAA AGCCTGACAT TAAAATGGAG CCGAGTTCAG GGCGTCCGGT TGATTATCAG
    ATCAGTGTTA CAGTAATCGA GGCCCGTCAG CTGGTCGGAT TGAACATGGA CCCTGTGGTT
    TGCGTGGAAA TTGGAGAGGA GAAGAAGTAC ACGTCAATGA AGGAGTCAAC AAACTGCCCT
    TACTACAATG AGTATTTTGT CTTCGACTTC CACGTGCCTC CAGACGTCAT GTTTGACAAA
    ATTATAAAGA TTTCAGTAAT ACATTCTAAA AACCTTCTGC GAAGCGGCAC CCTCGTGGGC
    ACCTTCAAAC TAGACGTGGG GACTGTTTAC ACACAACCTG AACATCAGTT TCATCACAAA
    TGGGCGATGC TGTCAGACCC TGATGACATC ACGACTGGCT GCAAAGGTTA TGTTAAATGT
    GACATCGCAG TCGTGGGAAA AGGAGACAAC ATCAAGACTC CACACAAGGC CAGTGAAGCA
    GACGAGGATG ACATAGAGGG GAATCTTCTC TTGCCGGAGG GTGTTCCATC AGAGAGACAG
    TGGGCTCGGT TTTATGTTAA GATTTATAGA GCTGAGGGTC TGCCTAAAAT GAACACCAGT
    ATCATGGCCA ACGTGAAGAA AGCCTTTATT GGGGAAAACA GGGACCTTGT GGACCCTTAC
    GTCCTGGTGC AATTTGCAGG GCAAAAGGGC AAAACATCTG TTCAGAAGAG CAGCTACGAG
    CCCATCTGGA ACGAGCAAGT CATCTTCACG GAGATGTTCC CACCTTTGTG TAGGCGACTG
    AAAGTTCAGA TCCGTGATTC AGACAAGGTG AACGATGTTG CCATAGGAAC CCACTTCATT
    GATCTACGAA AGGTTTCCAA TGATGGAGAC AAAGGTTTCT TGCCCACTAT GGGTCCGGCT
    TGGGTGAATA TGTATGGGTC GACCCGTAAC TACACCCTGA TGGACGAACA CCAGGACCTG
    AATGAGGGGT TGGGGGAAGG GGTGTCCTTC AGAGCACGTC TGCTCATTAG TATCGCGGTG
    GAGATTCTAG ACACCACCTC TGCTGAGATC ATGAGCTCCA CTGAAGTACA CATGGAGCCG
    GTGTCCAACA TCTCAGAGAG TGCTACAGGA AAGATGGAAG AATTTTTCCT TTTCGGGTCA
    TTCTTGGAGG CCACAATGAT AGACAGAAAA ATAGGAGATA AACCCATCAG CTTTGAAGTC
    ACCATCGGTA ACTATGGTAA CCAGATTGAT GGTGTGAGCA AGCCTGCATT AGCGAAGAAG
    AAGAAGGAGG GTGGAGGAGA GAGTGAAGAA GAGGAGTCTG AGCTTATCCA CAACTCCAGC
    GAGGAAGAGG CAGAGGATGA TGGAGATCTG ACCTCTGTAC CGTCTACACC TCCTATGAAG
    CCTGTTATCA CCGACAGAAA TTACTTCCAC TTGCCTTACT TTGAAAAGAA ACCCTGCATT
    TACATCAAGA GCTGGTGGCA GGACCAGCGG AGACGTCTCT ACAACTCCAA CATAATGGAC
    AAGATAGCTG ATAAACTGGA GGAAGGTCTG AATGATGTTC AAGAGATCAT AAAAACAGAG
    AAGGCATATC CAGAACGAAG ACTAAGAGGT GTATTAGAAG AACTTAGCAC AAGCTGCAGC
    CGGTTTGTTA CACTTGCAAA CAAGGATCAG AATCTTTCTG GAAGAACCAA GCTGGACAGA
    GAGAGGCTGA AGTCCTGTAT GAGAGAAATG GAGAGTATGG GTCAGCAGGC GAAGACCATC
    CGCACACAAG TGAAAAGAAA CACAGTTCGA GATAAACTCA AGCTAGTGTT GAATTTCCTT
    CAGAGGCTTC GATTCTTAGC AGACGAGCCC CAGCACAGCA TCCCTGATGT GTTCATATGG
    ATGATTAGCA ACAACAAACG CATCGCATAC GCCCGCATAC CCTCCAAAGA CATCCTCTAC
    TCAATTGTCG ATGAAGAGAT GGGCAAAGAC TGTGGAAAAG TTAAAGCTGT TTTTCTCAGG
    CTGCCTGGAA AGAAAGGTTT TGGGCCTGCT GGCTGGACAG TTCAGGCTAA ATTAGAGATG
    TATTTGTGGC TGGGCCTGAA TAAACAGAGG AAAGACTTCC TGATTGGCCT CCCTAGTGGC
    TTTGAAGAAA ACAAGGCAGT AAAGGGAATT GGCATACAAG CTGTCCCTCC CATCAGCCTA
    GTTTATAACA TGAAGCAGGT GTTTCAGCTG AGGGCCCACA TGTATCAAGC TCGCAGTCTG
    TTTGCTGCGG ACACTAGTGG CCTGTCTGAT CCATTTGCAA GGGTTTTCTT CTCCACCCAC
    AGCCAGGTCA CGGAGGTCCT CAGTGAGACT CTCTGTCCAA CATGGGATCA GCTGCTTGTG
    TTTGATAATG TTGAACTTTA CGGTGAGGCC GGTGAACTCC GTGATGACCC TCCAATCATT
    GTCATTGAGT TGTATGATCA AGACACTGTG GGGAAAGCTG AGTTTATTGG CCGAACATTT
    GCAAAACCAC TTACTAAGAT GGTTGATGAA CACTACGGCC CACCGCGGTT TCCTCCCCAG
    CTCGAGTATT ATCAGATCTA CCGAGGAAAC TGTGCTGCGG GAGATCTATT GGCTGCTTTT
    GAGCTTCTAC AGATTGGTCC AGCAGGGCGG GCAGCACTTC CTCCAATCGA TGGACCGACC
    GATTCTGACC GTGGGCCCAT TCTGCCTGTT CCATTGGGAA TTCGACCAGT TCTCAGCAGA
    TATCGCATCG AGGTCCTGTT TTGGGGTCTG AGGGACCTTA AAAGGGTCAA CCTGGCTCAG
    GTGGACCGAC CTCGTGTGGA CATTGAGTGT GCAGGGAAAG GAGTTCAGTC AGCCCTAATT
    CAAAACTACA AGAAGAATCC AAACTTCAGC ACATTAGTAA AGTGGTTTGA AGTGGATCTG
    CCAGAAAATG AGCTGCTCCA TCCACCACTT AATATTCGGG TAGTGGATTG CAGGGCATTC
    GGACGCTACA TTTTGGTGGG GTCTCATGCT GTGACCACCC TTCGCAAGTT CATCTACAGC
    CCCCCAGACA AGACTGCTAA CAACTGGGCT CACACAGCTA GACTGGCCAA TGGCTACCTG
    GCTCTCAGCA ATGGGACGTC TCATTCCCGC CCCCAATCCC GTCCTGTTTC TTATCCCTCA
    GGTGACATTG TGGTCAACAT GAGCCCTGAA CCTAACATTA AGAAGATGGA CACGGTTGTC
    AAGATTGAAG CTACCACCGA TGCGGTTGTT AAAGTTGACT TGAATGAGGA TGAGAAGGAG
    AAAGAAAAGA AGAAGAAGAA GAAGAAGAAA GGAGAAGAAG TGGAGGAAGA GGAGCCGGAT
    GAGAGCATGC TGGACTGGTG GTCCAAATAC TTTGCCTCAA TAGAGACTAT GATGGAGAAT
    CTAAGAGCTC AGGAGGCCGC TCAGGCAGAG GCAGAGGAAA GAGAAGATTT GGAGATTGCA
    GCAGAGAGTG CAGAGATCAA AGCTGATGAC TTTCCTATGA AAGGCACCAA ACCTAAAGAG
    AAGAGCAAAG ACAAGAAGAG CACCAAGGAC AAAAAGAAGA ACAACGATGG CACAGAAAAA
    CGACCTCCAA AACCAAAAGT TGATGAACTC ATGGTGTACA ATAAAGAGCT GGAGAGTGAG
    TTCGGTAGCT TTGAGGACTG GCTTCACACC TTCAACCTGT ACCGAGGAAA GGCTGGAGAT
    GATGATGACC ACAATGTGGT TGATGAGGAC AGAATTGTGG GCAGATTCAA GGGCTCCCTC
    TGTATGTATA AACTACCACT GTCTGAGGAG ATCACCAGAG AGGCAGGATT TGATCCAAAC
    ATGGGCATGT TCCAGAGTAT TCCTCACAAT GACCCTATCA ACGTTCTTGT CAGGATTTAT
    ATCATTCGAG CCACAGACCT GCATCCTGCA GATATAAATG GAAAGGCCGA CCCTTATATA
    GTCATTAAAT TGGGAAAGTC AGACATTCGA GACAAGGAGA ACTACATATC CAAGCAGCTG
    AATCCTGTCT TTGGAAAGTC ATTTGATATA GAGGCCACGT TCCCAATGGA GTCTATGCTG
    ACTGTGGCAG TGTATGACTG GGATTTAGTC GGAACTGATG ACCTGATTGG TGAGACTAAG
    ATTGATTTGG AGAACAGATA CTACAGCAAA CACAGAGCCA CATGTGGCAT TGCTTCCAAC
    TACTCTGTCC ATGGTTATAA TGTATGGAGA GATCCTCAGA AACCAGCTCA GATCCTGGCT
    AAGTTGTGCA AAGAAGGTAA ACTGGATGGA CCTCACTATG GACCTGGGGG AAGAGTCAAA
    GTTGCAAACC GGATCTTTCT TGGGCCGACA GAAATTGAGG ATGAGAGTGG TCTGAAAAAA
    CAAACAGAAG AACACTTGGC GCTCACAGTC CTTAGACATT GGGAGGAAAT CCCACGTGTT
    GGTTGCAAAC TCATCCCTGA GCATGTGGAG ACGAGGCCTC TCCTCAACCC AGACAAGCCA
    GGCATAGAGC AGGGAAGGAT TGAAATGTGG GTGGACATGT TTCCTATGGA TGTCCCAGCA
    CCAGGACCAG CCATTGATAT ATCGCCACGC AAACCAAAGA GATATGAGCT CAGGGTGATA
    ATATGGAATA CTGACGAGGT AATTCTGGAA GACGATGATT ACTTCACAGG GGAAAAGTCC
    AGTGACATTT TTGTGAGGGG CTGGCTTAAA GGACAGCAGG AGGACAAACA GGACACCGAT
    GTACATTATC ACTCTCTAAC TGGAGAGGGA AACTTCAACT GGCGCTTTGT CTTCCCCTTT
    GACTATCTCA TGGCCGAGGA GAAGATTGTT ATCTCCAAGA AGGAATCAAT GTTTTCCTGG
    GATGAGACTG AGTATAAGAT CCCTGCTCGA CTCACCCTTC AAGTATGGGA TGCCGATCAC
    TTTTCTGCAG ATGATTTTCT GGGTGCGATT GAGTTGGACT TGAATAAGTT TCCGCGTGGG
    GCAAAAACAG CCAAGCAGTG CTCTCTAGAC ATGGTTCTCA AAGAGCATGA GCTGCCAACC
    ATCTCCATCT TCAAACAAAA GAGAGTCAAG GGCTGGTGGC CATTTGTGGC CCAAAATGAG
    AATGATGAAT TTGAGCTAAC GGGAAAAGTA GAGGCAGAGC TCCATCTGTT GACAGCAGAG
    GAGGCAGAGA AGAGTCCAGT CGGCCTGGGG AGGAATGATC CGGAGCCACT TGAGAAACCA
    AATCGTCCAG ACACCAGTCT AATGTGGTTC ATGAACCCTC TGAGGTCCAT ACGATACTTC
    ATTTGGCACA ACTACCGCTG GCTGATCCTG AAGGCTCTGG CTCTGCTGCT GCTCCTTCTC
    CTCGTTGGGC TCTTCCTCTA CTCTATTCCT GGATACCTGG TCAAGAAACT CCTAGGGGCT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021324118.1
    CDS300..6362
    Translation

    Target ORF information:

    RefSeq Version XM_021468443.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio otoferlin a (otofa), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021468443.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGGCTTTAG TGGTGCATCT GAAGACTGTC ACGGAGCTCC GTGGGAAGGG GGACCGAATC 
    GCTAAAGTCA CTTTCAGAGG TCTCTCCTTC TTCTCTCGGG TGTTGGAAAA CTGCGAGGAT
    GAAGCTCGAT TTGAGCAGGC GTTCCGATGG CCAATTGGGA GTCAAGTGGA TGGCGACGAG
    ATGCTGGAGA TCCAAGTGTT TAATTACAGC AAAGTCTTCA CTAATAGGTT AATCGGCACA
    TTCCGCATGG TTCTTCAGAA GGTGGTGGAG GAGGGACACC TGGAGGTGTC TGACACACTC
    ATTGACGACA ATAACACAGC CATACAGACA ACCATTTCCA TAGAGATCAA ATATCAGACC
    ATGGACGGGA GTGTAAAGGT GTGGAGCGAT GGTGAATTCC TTGATATTCC AGATGACCTT
    GATGGAACCT TCCAGTTTGA GACAGATAGT CTTCTCTCAG GACGCAGCCA GAGTTCTGGA
    ACTTCACCTG GACGATCCAT TCATGGCATT CCCACATTCC GCAAGGCAGG AAAAGGAGTG
    TTCTCAGCCA TGAAGCTGGG CAAGACCCGC ACCTCTAAAG ATGACCACAA AAAAGGAGAT
    GATGCTGCCA TTCTGGATGC AGAAGATCTG GATCGAAAAG CCATGCGTCT AGGCGGCATT
    CTTGACCCTG ATACAATCTC ACTGGCCTCG GTTACTGCAG TGACCACTAA TGTCTCCAAC
    AAAAGATCAA AGCCTGACAT TAAAATGGAG CCGAGTTCAG GGCGTCCGGT TGATTATCAG
    ATCAGTGTTA CAGTAATCGA GGCCCGTCAG CTGGTCGGAT TGAACATGGA CCCTGTGGTT
    TGCGTGGAAA TTGGAGAGGA GAAGAAGTAC ACGTCAATGA AGGAGTCAAC AAACTGCCCT
    TACTACAATG AGTATTTTGT CTTCGACTTC CACGTGCCTC CAGACGTCAT GTTTGACAAA
    ATTATAAAGA TTTCAGTAAT ACATTCTAAA AACCTTCTGC GAAGCGGCAC CCTCGTGGGC
    ACCTTCAAAC TAGACGTGGG GACTGTTTAC ACACAACCTG AACATCAGTT TCATCACAAA
    TGGGCGATGC TGTCAGACCC TGATGACATC ACGACTGGCT GCAAAGGTTA TGTTAAATGT
    GACATCGCAG TCGTGGGAAA AGGAGACAAC ATCAAGACTC CACACAAGGC CAGTGAAGCA
    GACGAGGATG ACATAGAGGG GAATCTTCTC TTGCCGGAGG GTGTTCCATC AGAGAGACAG
    TGGGCTCGGT TTTATGTTAA GATTTATAGA GCTGAGGGTC TGCCTAAAAT GAACACCAGT
    ATCATGGCCA ACGTGAAGAA AGCCTTTATT GGGGAAAACA GGGACCTTGT GGACCCTTAC
    GTCCTGGTGC AATTTGCAGG GCAAAAGGGC AAAACATCTG TTCAGAAGAG CAGCTACGAG
    CCCATCTGGA ACGAGCAAGT CATCTTCACG GAGATGTTCC CACCTTTGTG TAGGCGACTG
    AAAGTTCAGA TCCGTGATTC AGACAAGGTG AACGATGTTG CCATAGGAAC CCACTTCATT
    GATCTACGAA AGGTTTCCAA TGATGGAGAC AAAGGTTTCT TGCCCACTAT GGGTCCGGCT
    TGGGTGAATA TGTATGGGTC GACCCGTAAC TACACCCTGA TGGACGAACA CCAGGACCTG
    AATGAGGGGT TGGGGGAAGG GGTGTCCTTC AGAGCACGTC TGCTCATTAG TATCGCGGTG
    GAGATTCTAG ACACCACCTC TGCTGAGATC ATGAGCTCCA CTGAAGTACA CATGGAGCCG
    GTGTCCAACA TCTCAGAGAG TGCTACAGGA AAGATGGAAG AATTTTTCCT TTTCGGGTCA
    TTCTTGGAGG CCACAATGAT AGACAGAAAA ATAGGAGATA AACCCATCAG CTTTGAAGTC
    ACCATCGGTA ACTATGGTAA CCAGATTGAT GGTGTGAGCA AGCCTGCATT AGCGAAGAAG
    AAGAAGGAGG GTGGAGGAGA GAGTGAAGAA GAGGAGTCTG AGCTTATCCA CAACTCCAGC
    GAGGAAGAGG CAGAGGATGA TGGAGATCTG ACCTCTGTAC CGTCTACACC TCCTATGAAG
    CCTGTTATCA CCGACAGAAA TTACTTCCAC TTGCCTTACT TTGAAAAGAA ACCCTGCATT
    TACATCAAGA GCTGGTGGCA GGACCAGCGG AGACGTCTCT ACAACTCCAA CATAATGGAC
    AAGATAGCTG ATAAACTGGA GGAAGGTCTG AATGATGTTC AAGAGATCAT AAAAACAGAG
    AAGGCATATC CAGAACGAAG ACTAAGAGGT GTATTAGAAG AACTTAGCAC AAGCTGCAGC
    CGGTTTGTTA CACTTGCAAA CAAGGATCAG AATCTTTCTG GAAGAACCAA GCTGGACAGA
    GAGAGGCTGA AGTCCTGTAT GAGAGAAATG GAGAGTATGG GTCAGCAGGC GAAGACCATC
    CGCACACAAG TGAAAAGAAA CACAGTTCGA GATAAACTCA AGCTAGTGTT GAATTTCCTT
    CAGAGGCTTC GATTCTTAGC AGACGAGCCC CAGCACAGCA TCCCTGATGT GTTCATATGG
    ATGATTAGCA ACAACAAACG CATCGCATAC GCCCGCATAC CCTCCAAAGA CATCCTCTAC
    TCAATTGTCG ATGAAGAGAT GGGCAAAGAC TGTGGAAAAG TTAAAGCTGT TTTTCTCAGG
    CTGCCTGGAA AGAAAGGTTT TGGGCCTGCT GGCTGGACAG TTCAGGCTAA ATTAGAGATG
    TATTTGTGGC TGGGCCTGAA TAAACAGAGG AAAGACTTCC TGATTGGCCT CCCTAGTGGC
    TTTGAAGAAA ACAAGGCAGT AAAGGGAATT GGCATACAAG CTGTCCCTCC CATCAGCCTA
    GTTTATAACA TGAAGCAGGT GTTTCAGCTG AGGGCCCACA TGTATCAAGC TCGCAGTCTG
    TTTGCTGCGG ACACTAGTGG CCTGTCTGAT CCATTTGCAA GGGTTTTCTT CTCCACCCAC
    AGCCAGGTCA CGGAGGTCCT CAGTGAGACT CTCTGTCCAA CATGGGATCA GCTGCTTGTG
    TTTGATAATG TTGAACTTTA CGGTGAGGCC GGTGAACTCC GTGATGACCC TCCAATCATT
    GTCATTGAGT TGTATGATCA AGACACTGTG GGGAAAGCTG AGTTTATTGG CCGAACATTT
    GCAAAACCAC TTACTAAGAT GGTTGATGAA CACTACGGCC CACCGCGGTT TCCTCCCCAG
    CTCGAGTATT ATCAGATCTA CCGAGGAAAC TGTGCTGCGG GAGATCTATT GGCTGCTTTT
    GAGCTTCTAC AGATTGGTCC AGCAGGGCGG GCAGCACTTC CTCCAATCGA TGGACCGACC
    GATTCTGACC GTGGGCCCAT TCTGCCTGTT CCATTGGGAA TTCGACCAGT TCTCAGCAGA
    TATCGCATCG AGGTCCTGTT TTGGGGTCTG AGGGACCTTA AAAGGGTCAA CCTGGCTCAG
    GTGGACCGAC CTCGTGTGGA CATTGAGTGT GCAGGGAAAG GAGTTCAGTC AGCCCTAATT
    CAAAACTACA AGAAGAATCC AAACTTCAGC ACATTAGTAA AGTGGTTTGA AGTGGATCTG
    CCAGAAAATG AGCTGCTCCA TCCACCACTT AATATTCGGG TAGTGGATTG CAGGGCATTC
    GGACGCTACA TTTTGGTGGG GTCTCATGCT GTGACCACCC TTCGCAAGTT CATCTACAGC
    CCCCCAGACA AGACTGCTAA CAACTGGGCT CACACAGCTA GACTGGCCAA TGGCTACCTG
    GCTCTCAGCA ATGGGACGTC TCATTCCCGC CCCCAATCCC GTCCTGTTTC TTATCCCTCA
    GGTGACATTG TGGTCAACAT GAGCCCTGAA CCTAACATTA AGAAGATGGA CACGGTTGTC
    AAGATTGAAG CTACCACCGA TGCGGTTGTT AAAGTTGACT TGAATGAGGA TGAGAAGGAG
    AAAGAAAAGA AGAAGAAGAA GAAGAAGAAA GGAGAAGAAG TGGAGGAAGA GGAGCCGGAT
    GAGAGCATGC TGGACTGGTG GTCCAAATAC TTTGCCTCAA TAGAGACTAT GATGGAGAAT
    CTAAGAGCTC AGGAGGCCGC TCAGGCAGAG GCAGAGGAAA GAGAAGATTT GGAGATTGCA
    GCAGAGAGTG CAGAGATCAA AGCTGATGAC TTTCCTATGA AAGGCACCAA ACCTAAAGAG
    AAGAGCAAAG ACAAGAAGAG CACCAAGGAC AAAAAGAAGA ACAACGATGG CACAGAAAAA
    CGACCTCCAA AACCAAAAGT TGATGAACTC ATGGTGTACA ATAAAGAGCT GGAGAGTGAG
    TTCGGTAGCT TTGAGGACTG GCTTCACACC TTCAACCTGT ACCGAGGAAA GGCTGGAGAT
    GATGATGACC ACAATGTGGT TGATGAGGAC AGAATTGTGG GCAGATTCAA GGGCTCCCTC
    TGTATGTATA AACTACCACT GTCTGAGGAG ATCACCAGAG AGGCAGGATT TGATCCAAAC
    ATGGGCATGT TCCAGAGTAT TCCTCACAAT GACCCTATCA ACGTTCTTGT CAGGATTTAT
    ATCATTCGAG CCACAGACCT GCATCCTGCA GATATAAATG GAAAGGCCGA CCCTTATATA
    GTCATTAAAT TGGGAAAGTC AGACATTCGA GACAAGGAGA ACTACATATC CAAGCAGCTG
    AATCCTGTCT TTGGAAAGTC ATTTGATATA GAGGCCACGT TCCCAATGGA GTCTATGCTG
    ACTGTGGCAG TGTATGACTG GGATTTAGTC GGAACTGATG ACCTGATTGG TGAGACTAAG
    ATTGATTTGG AGAACAGATA CTACAGCAAA CACAGAGCCA CATGTGGCAT TGCTTCCAAC
    TACTCTGTCC ATGGTTATAA TGTATGGAGA GATCCTCAGA AACCAGCTCA GATCCTGGCT
    AAGTTGTGCA AAGAAGGTAA ACTGGATGGA CCTCACTATG GACCTGGGGG AAGAGTCAAA
    GTTGCAAACC GGATCTTTCT TGGGCCGACA GAAATTGAGG ATGAGAGTGG TCTGAAAAAA
    CAAACAGAAG AACACTTGGC GCTCACAGTC CTTAGACATT GGGAGGAAAT CCCACGTGTT
    GGTTGCAAAC TCATCCCTGA GCATGTGGAG ACGAGGCCTC TCCTCAACCC AGACAAGCCA
    GGCATAGAGC AGGGAAGGAT TGAAATGTGG GTGGACATGT TTCCTATGGA TGTCCCAGCA
    CCAGGACCAG CCATTGATAT ATCGCCACGC AAACCAAAGA GATATGAGCT CAGGGTGATA
    ATATGGAATA CTGACGAGGT AATTCTGGAA GACGATGATT ACTTCACAGG GGAAAAGTCC
    AGTGACATTT TTGTGAGGGG CTGGCTTAAA GGACAGCAGG AGGACAAACA GGACACCGAT
    GTACATTATC ACTCTCTAAC TGGAGAGGGA AACTTCAACT GGCGCTTTGT CTTCCCCTTT
    GACTATCTCA TGGCCGAGGA GAAGATTGTT ATCTCCAAGA AGGAATCAAT GTTTTCCTGG
    GATGAGACTG AGTATAAGAT CCCTGCTCGA CTCACCCTTC AAGTATGGGA TGCCGATCAC
    TTTTCTGCAG ATGATTTTCT GGGTGCGATT GAGTTGGACT TGAATAAGTT TCCGCGTGGG
    GCAAAAACAG CCAAGCAGTG CTCTCTAGAC ATGGTTCTCA AAGAGCATGA GCTGCCAACC
    ATCTCCATCT TCAAACAAAA GAGAGTCAAG GGCTGGTGGC CATTTGTGGC CCAAAATGAG
    AATGATGAAT TTGAGCTAAC GGGAAAAGTA GAGGCAGAGC TCCATCTGTT GACAGCAGAG
    GAGGCAGAGA AGAGTCCAGT CGGCCTGGGG AGGAATGATC CGGAGCCACT TGAGAAACCA
    AATCGTCCAG ACACCAGTCT AATGTGGTTC ATGAACCCTC TGAGGTCCAT ACGATACTTC
    ATTTGGCACA ACTACCGCTG GCTGATCCTG AAGGCTCTGG CTCTGCTGCT GCTCCTTCTC
    CTCGTTGGGC TCTTCCTCTA CTCTATTCCT GGATACCTGG TCAAGAAACT CCTAGGGGCT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42614
    Clone ID Related Accession (Same CDS sequence) XM_021468443.1 , XM_009294743.2
    Accession Version XM_009294743.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6063bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product otoferlin isoform X9
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009294743.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGGCTTTAG TGGTGCATCT GAAGACTGTC ACGGAGCTCC GTGGGAAGGG GGACCGAATC 
    GCTAAAGTCA CTTTCAGAGG TCTCTCCTTC TTCTCTCGGG TGTTGGAAAA CTGCGAGGAT
    GAAGCTCGAT TTGAGCAGGC GTTCCGATGG CCAATTGGGA GTCAAGTGGA TGGCGACGAG
    ATGCTGGAGA TCCAAGTGTT TAATTACAGC AAAGTCTTCA CTAATAGGTT AATCGGCACA
    TTCCGCATGG TTCTTCAGAA GGTGGTGGAG GAGGGACACC TGGAGGTGTC TGACACACTC
    ATTGACGACA ATAACACAGC CATACAGACA ACCATTTCCA TAGAGATCAA ATATCAGACC
    ATGGACGGGA GTGTAAAGGT GTGGAGCGAT GGTGAATTCC TTGATATTCC AGATGACCTT
    GATGGAACCT TCCAGTTTGA GACAGATAGT CTTCTCTCAG GACGCAGCCA GAGTTCTGGA
    ACTTCACCTG GACGATCCAT TCATGGCATT CCCACATTCC GCAAGGCAGG AAAAGGAGTG
    TTCTCAGCCA TGAAGCTGGG CAAGACCCGC ACCTCTAAAG ATGACCACAA AAAAGGAGAT
    GATGCTGCCA TTCTGGATGC AGAAGATCTG GATCGAAAAG CCATGCGTCT AGGCGGCATT
    CTTGACCCTG ATACAATCTC ACTGGCCTCG GTTACTGCAG TGACCACTAA TGTCTCCAAC
    AAAAGATCAA AGCCTGACAT TAAAATGGAG CCGAGTTCAG GGCGTCCGGT TGATTATCAG
    ATCAGTGTTA CAGTAATCGA GGCCCGTCAG CTGGTCGGAT TGAACATGGA CCCTGTGGTT
    TGCGTGGAAA TTGGAGAGGA GAAGAAGTAC ACGTCAATGA AGGAGTCAAC AAACTGCCCT
    TACTACAATG AGTATTTTGT CTTCGACTTC CACGTGCCTC CAGACGTCAT GTTTGACAAA
    ATTATAAAGA TTTCAGTAAT ACATTCTAAA AACCTTCTGC GAAGCGGCAC CCTCGTGGGC
    ACCTTCAAAC TAGACGTGGG GACTGTTTAC ACACAACCTG AACATCAGTT TCATCACAAA
    TGGGCGATGC TGTCAGACCC TGATGACATC ACGACTGGCT GCAAAGGTTA TGTTAAATGT
    GACATCGCAG TCGTGGGAAA AGGAGACAAC ATCAAGACTC CACACAAGGC CAGTGAAGCA
    GACGAGGATG ACATAGAGGG GAATCTTCTC TTGCCGGAGG GTGTTCCATC AGAGAGACAG
    TGGGCTCGGT TTTATGTTAA GATTTATAGA GCTGAGGGTC TGCCTAAAAT GAACACCAGT
    ATCATGGCCA ACGTGAAGAA AGCCTTTATT GGGGAAAACA GGGACCTTGT GGACCCTTAC
    GTCCTGGTGC AATTTGCAGG GCAAAAGGGC AAAACATCTG TTCAGAAGAG CAGCTACGAG
    CCCATCTGGA ACGAGCAAGT CATCTTCACG GAGATGTTCC CACCTTTGTG TAGGCGACTG
    AAAGTTCAGA TCCGTGATTC AGACAAGGTG AACGATGTTG CCATAGGAAC CCACTTCATT
    GATCTACGAA AGGTTTCCAA TGATGGAGAC AAAGGTTTCT TGCCCACTAT GGGTCCGGCT
    TGGGTGAATA TGTATGGGTC GACCCGTAAC TACACCCTGA TGGACGAACA CCAGGACCTG
    AATGAGGGGT TGGGGGAAGG GGTGTCCTTC AGAGCACGTC TGCTCATTAG TATCGCGGTG
    GAGATTCTAG ACACCACCTC TGCTGAGATC ATGAGCTCCA CTGAAGTACA CATGGAGCCG
    GTGTCCAACA TCTCAGAGAG TGCTACAGGA AAGATGGAAG AATTTTTCCT TTTCGGGTCA
    TTCTTGGAGG CCACAATGAT AGACAGAAAA ATAGGAGATA AACCCATCAG CTTTGAAGTC
    ACCATCGGTA ACTATGGTAA CCAGATTGAT GGTGTGAGCA AGCCTGCATT AGCGAAGAAG
    AAGAAGGAGG GTGGAGGAGA GAGTGAAGAA GAGGAGTCTG AGCTTATCCA CAACTCCAGC
    GAGGAAGAGG CAGAGGATGA TGGAGATCTG ACCTCTGTAC CGTCTACACC TCCTATGAAG
    CCTGTTATCA CCGACAGAAA TTACTTCCAC TTGCCTTACT TTGAAAAGAA ACCCTGCATT
    TACATCAAGA GCTGGTGGCA GGACCAGCGG AGACGTCTCT ACAACTCCAA CATAATGGAC
    AAGATAGCTG ATAAACTGGA GGAAGGTCTG AATGATGTTC AAGAGATCAT AAAAACAGAG
    AAGGCATATC CAGAACGAAG ACTAAGAGGT GTATTAGAAG AACTTAGCAC AAGCTGCAGC
    CGGTTTGTTA CACTTGCAAA CAAGGATCAG AATCTTTCTG GAAGAACCAA GCTGGACAGA
    GAGAGGCTGA AGTCCTGTAT GAGAGAAATG GAGAGTATGG GTCAGCAGGC GAAGACCATC
    CGCACACAAG TGAAAAGAAA CACAGTTCGA GATAAACTCA AGCTAGTGTT GAATTTCCTT
    CAGAGGCTTC GATTCTTAGC AGACGAGCCC CAGCACAGCA TCCCTGATGT GTTCATATGG
    ATGATTAGCA ACAACAAACG CATCGCATAC GCCCGCATAC CCTCCAAAGA CATCCTCTAC
    TCAATTGTCG ATGAAGAGAT GGGCAAAGAC TGTGGAAAAG TTAAAGCTGT TTTTCTCAGG
    CTGCCTGGAA AGAAAGGTTT TGGGCCTGCT GGCTGGACAG TTCAGGCTAA ATTAGAGATG
    TATTTGTGGC TGGGCCTGAA TAAACAGAGG AAAGACTTCC TGATTGGCCT CCCTAGTGGC
    TTTGAAGAAA ACAAGGCAGT AAAGGGAATT GGCATACAAG CTGTCCCTCC CATCAGCCTA
    GTTTATAACA TGAAGCAGGT GTTTCAGCTG AGGGCCCACA TGTATCAAGC TCGCAGTCTG
    TTTGCTGCGG ACACTAGTGG CCTGTCTGAT CCATTTGCAA GGGTTTTCTT CTCCACCCAC
    AGCCAGGTCA CGGAGGTCCT CAGTGAGACT CTCTGTCCAA CATGGGATCA GCTGCTTGTG
    TTTGATAATG TTGAACTTTA CGGTGAGGCC GGTGAACTCC GTGATGACCC TCCAATCATT
    GTCATTGAGT TGTATGATCA AGACACTGTG GGGAAAGCTG AGTTTATTGG CCGAACATTT
    GCAAAACCAC TTACTAAGAT GGTTGATGAA CACTACGGCC CACCGCGGTT TCCTCCCCAG
    CTCGAGTATT ATCAGATCTA CCGAGGAAAC TGTGCTGCGG GAGATCTATT GGCTGCTTTT
    GAGCTTCTAC AGATTGGTCC AGCAGGGCGG GCAGCACTTC CTCCAATCGA TGGACCGACC
    GATTCTGACC GTGGGCCCAT TCTGCCTGTT CCATTGGGAA TTCGACCAGT TCTCAGCAGA
    TATCGCATCG AGGTCCTGTT TTGGGGTCTG AGGGACCTTA AAAGGGTCAA CCTGGCTCAG
    GTGGACCGAC CTCGTGTGGA CATTGAGTGT GCAGGGAAAG GAGTTCAGTC AGCCCTAATT
    CAAAACTACA AGAAGAATCC AAACTTCAGC ACATTAGTAA AGTGGTTTGA AGTGGATCTG
    CCAGAAAATG AGCTGCTCCA TCCACCACTT AATATTCGGG TAGTGGATTG CAGGGCATTC
    GGACGCTACA TTTTGGTGGG GTCTCATGCT GTGACCACCC TTCGCAAGTT CATCTACAGC
    CCCCCAGACA AGACTGCTAA CAACTGGGCT CACACAGCTA GACTGGCCAA TGGCTACCTG
    GCTCTCAGCA ATGGGACGTC TCATTCCCGC CCCCAATCCC GTCCTGTTTC TTATCCCTCA
    GGTGACATTG TGGTCAACAT GAGCCCTGAA CCTAACATTA AGAAGATGGA CACGGTTGTC
    AAGATTGAAG CTACCACCGA TGCGGTTGTT AAAGTTGACT TGAATGAGGA TGAGAAGGAG
    AAAGAAAAGA AGAAGAAGAA GAAGAAGAAA GGAGAAGAAG TGGAGGAAGA GGAGCCGGAT
    GAGAGCATGC TGGACTGGTG GTCCAAATAC TTTGCCTCAA TAGAGACTAT GATGGAGAAT
    CTAAGAGCTC AGGAGGCCGC TCAGGCAGAG GCAGAGGAAA GAGAAGATTT GGAGATTGCA
    GCAGAGAGTG CAGAGATCAA AGCTGATGAC TTTCCTATGA AAGGCACCAA ACCTAAAGAG
    AAGAGCAAAG ACAAGAAGAG CACCAAGGAC AAAAAGAAGA ACAACGATGG CACAGAAAAA
    CGACCTCCAA AACCAAAAGT TGATGAACTC ATGGTGTACA ATAAAGAGCT GGAGAGTGAG
    TTCGGTAGCT TTGAGGACTG GCTTCACACC TTCAACCTGT ACCGAGGAAA GGCTGGAGAT
    GATGATGACC ACAATGTGGT TGATGAGGAC AGAATTGTGG GCAGATTCAA GGGCTCCCTC
    TGTATGTATA AACTACCACT GTCTGAGGAG ATCACCAGAG AGGCAGGATT TGATCCAAAC
    ATGGGCATGT TCCAGAGTAT TCCTCACAAT GACCCTATCA ACGTTCTTGT CAGGATTTAT
    ATCATTCGAG CCACAGACCT GCATCCTGCA GATATAAATG GAAAGGCCGA CCCTTATATA
    GTCATTAAAT TGGGAAAGTC AGACATTCGA GACAAGGAGA ACTACATATC CAAGCAGCTG
    AATCCTGTCT TTGGAAAGTC ATTTGATATA GAGGCCACGT TCCCAATGGA GTCTATGCTG
    ACTGTGGCAG TGTATGACTG GGATTTAGTC GGAACTGATG ACCTGATTGG TGAGACTAAG
    ATTGATTTGG AGAACAGATA CTACAGCAAA CACAGAGCCA CATGTGGCAT TGCTTCCAAC
    TACTCTGTCC ATGGTTATAA TGTATGGAGA GATCCTCAGA AACCAGCTCA GATCCTGGCT
    AAGTTGTGCA AAGAAGGTAA ACTGGATGGA CCTCACTATG GACCTGGGGG AAGAGTCAAA
    GTTGCAAACC GGATCTTTCT TGGGCCGACA GAAATTGAGG ATGAGAGTGG TCTGAAAAAA
    CAAACAGAAG AACACTTGGC GCTCACAGTC CTTAGACATT GGGAGGAAAT CCCACGTGTT
    GGTTGCAAAC TCATCCCTGA GCATGTGGAG ACGAGGCCTC TCCTCAACCC AGACAAGCCA
    GGCATAGAGC AGGGAAGGAT TGAAATGTGG GTGGACATGT TTCCTATGGA TGTCCCAGCA
    CCAGGACCAG CCATTGATAT ATCGCCACGC AAACCAAAGA GATATGAGCT CAGGGTGATA
    ATATGGAATA CTGACGAGGT AATTCTGGAA GACGATGATT ACTTCACAGG GGAAAAGTCC
    AGTGACATTT TTGTGAGGGG CTGGCTTAAA GGACAGCAGG AGGACAAACA GGACACCGAT
    GTACATTATC ACTCTCTAAC TGGAGAGGGA AACTTCAACT GGCGCTTTGT CTTCCCCTTT
    GACTATCTCA TGGCCGAGGA GAAGATTGTT ATCTCCAAGA AGGAATCAAT GTTTTCCTGG
    GATGAGACTG AGTATAAGAT CCCTGCTCGA CTCACCCTTC AAGTATGGGA TGCCGATCAC
    TTTTCTGCAG ATGATTTTCT GGGTGCGATT GAGTTGGACT TGAATAAGTT TCCGCGTGGG
    GCAAAAACAG CCAAGCAGTG CTCTCTAGAC ATGGTTCTCA AAGAGCATGA GCTGCCAACC
    ATCTCCATCT TCAAACAAAA GAGAGTCAAG GGCTGGTGGC CATTTGTGGC CCAAAATGAG
    AATGATGAAT TTGAGCTAAC GGGAAAAGTA GAGGCAGAGC TCCATCTGTT GACAGCAGAG
    GAGGCAGAGA AGAGTCCAGT CGGCCTGGGG AGGAATGATC CGGAGCCACT TGAGAAACCA
    AATCGTCCAG ACACCAGTCT AATGTGGTTC ATGAACCCTC TGAGGTCCAT ACGATACTTC
    ATTTGGCACA ACTACCGCTG GCTGATCCTG AAGGCTCTGG CTCTGCTGCT GCTCCTTCTC
    CTCGTTGGGC TCTTCCTCTA CTCTATTCCT GGATACCTGG TCAAGAAACT CCTAGGGGCT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009293018.1
    CDS303..6365
    Translation

    Target ORF information:

    RefSeq Version XM_009294743.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio otoferlin a (otofa), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009294743.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGGCTTTAG TGGTGCATCT GAAGACTGTC ACGGAGCTCC GTGGGAAGGG GGACCGAATC 
    GCTAAAGTCA CTTTCAGAGG TCTCTCCTTC TTCTCTCGGG TGTTGGAAAA CTGCGAGGAT
    GAAGCTCGAT TTGAGCAGGC GTTCCGATGG CCAATTGGGA GTCAAGTGGA TGGCGACGAG
    ATGCTGGAGA TCCAAGTGTT TAATTACAGC AAAGTCTTCA CTAATAGGTT AATCGGCACA
    TTCCGCATGG TTCTTCAGAA GGTGGTGGAG GAGGGACACC TGGAGGTGTC TGACACACTC
    ATTGACGACA ATAACACAGC CATACAGACA ACCATTTCCA TAGAGATCAA ATATCAGACC
    ATGGACGGGA GTGTAAAGGT GTGGAGCGAT GGTGAATTCC TTGATATTCC AGATGACCTT
    GATGGAACCT TCCAGTTTGA GACAGATAGT CTTCTCTCAG GACGCAGCCA GAGTTCTGGA
    ACTTCACCTG GACGATCCAT TCATGGCATT CCCACATTCC GCAAGGCAGG AAAAGGAGTG
    TTCTCAGCCA TGAAGCTGGG CAAGACCCGC ACCTCTAAAG ATGACCACAA AAAAGGAGAT
    GATGCTGCCA TTCTGGATGC AGAAGATCTG GATCGAAAAG CCATGCGTCT AGGCGGCATT
    CTTGACCCTG ATACAATCTC ACTGGCCTCG GTTACTGCAG TGACCACTAA TGTCTCCAAC
    AAAAGATCAA AGCCTGACAT TAAAATGGAG CCGAGTTCAG GGCGTCCGGT TGATTATCAG
    ATCAGTGTTA CAGTAATCGA GGCCCGTCAG CTGGTCGGAT TGAACATGGA CCCTGTGGTT
    TGCGTGGAAA TTGGAGAGGA GAAGAAGTAC ACGTCAATGA AGGAGTCAAC AAACTGCCCT
    TACTACAATG AGTATTTTGT CTTCGACTTC CACGTGCCTC CAGACGTCAT GTTTGACAAA
    ATTATAAAGA TTTCAGTAAT ACATTCTAAA AACCTTCTGC GAAGCGGCAC CCTCGTGGGC
    ACCTTCAAAC TAGACGTGGG GACTGTTTAC ACACAACCTG AACATCAGTT TCATCACAAA
    TGGGCGATGC TGTCAGACCC TGATGACATC ACGACTGGCT GCAAAGGTTA TGTTAAATGT
    GACATCGCAG TCGTGGGAAA AGGAGACAAC ATCAAGACTC CACACAAGGC CAGTGAAGCA
    GACGAGGATG ACATAGAGGG GAATCTTCTC TTGCCGGAGG GTGTTCCATC AGAGAGACAG
    TGGGCTCGGT TTTATGTTAA GATTTATAGA GCTGAGGGTC TGCCTAAAAT GAACACCAGT
    ATCATGGCCA ACGTGAAGAA AGCCTTTATT GGGGAAAACA GGGACCTTGT GGACCCTTAC
    GTCCTGGTGC AATTTGCAGG GCAAAAGGGC AAAACATCTG TTCAGAAGAG CAGCTACGAG
    CCCATCTGGA ACGAGCAAGT CATCTTCACG GAGATGTTCC CACCTTTGTG TAGGCGACTG
    AAAGTTCAGA TCCGTGATTC AGACAAGGTG AACGATGTTG CCATAGGAAC CCACTTCATT
    GATCTACGAA AGGTTTCCAA TGATGGAGAC AAAGGTTTCT TGCCCACTAT GGGTCCGGCT
    TGGGTGAATA TGTATGGGTC GACCCGTAAC TACACCCTGA TGGACGAACA CCAGGACCTG
    AATGAGGGGT TGGGGGAAGG GGTGTCCTTC AGAGCACGTC TGCTCATTAG TATCGCGGTG
    GAGATTCTAG ACACCACCTC TGCTGAGATC ATGAGCTCCA CTGAAGTACA CATGGAGCCG
    GTGTCCAACA TCTCAGAGAG TGCTACAGGA AAGATGGAAG AATTTTTCCT TTTCGGGTCA
    TTCTTGGAGG CCACAATGAT AGACAGAAAA ATAGGAGATA AACCCATCAG CTTTGAAGTC
    ACCATCGGTA ACTATGGTAA CCAGATTGAT GGTGTGAGCA AGCCTGCATT AGCGAAGAAG
    AAGAAGGAGG GTGGAGGAGA GAGTGAAGAA GAGGAGTCTG AGCTTATCCA CAACTCCAGC
    GAGGAAGAGG CAGAGGATGA TGGAGATCTG ACCTCTGTAC CGTCTACACC TCCTATGAAG
    CCTGTTATCA CCGACAGAAA TTACTTCCAC TTGCCTTACT TTGAAAAGAA ACCCTGCATT
    TACATCAAGA GCTGGTGGCA GGACCAGCGG AGACGTCTCT ACAACTCCAA CATAATGGAC
    AAGATAGCTG ATAAACTGGA GGAAGGTCTG AATGATGTTC AAGAGATCAT AAAAACAGAG
    AAGGCATATC CAGAACGAAG ACTAAGAGGT GTATTAGAAG AACTTAGCAC AAGCTGCAGC
    CGGTTTGTTA CACTTGCAAA CAAGGATCAG AATCTTTCTG GAAGAACCAA GCTGGACAGA
    GAGAGGCTGA AGTCCTGTAT GAGAGAAATG GAGAGTATGG GTCAGCAGGC GAAGACCATC
    CGCACACAAG TGAAAAGAAA CACAGTTCGA GATAAACTCA AGCTAGTGTT GAATTTCCTT
    CAGAGGCTTC GATTCTTAGC AGACGAGCCC CAGCACAGCA TCCCTGATGT GTTCATATGG
    ATGATTAGCA ACAACAAACG CATCGCATAC GCCCGCATAC CCTCCAAAGA CATCCTCTAC
    TCAATTGTCG ATGAAGAGAT GGGCAAAGAC TGTGGAAAAG TTAAAGCTGT TTTTCTCAGG
    CTGCCTGGAA AGAAAGGTTT TGGGCCTGCT GGCTGGACAG TTCAGGCTAA ATTAGAGATG
    TATTTGTGGC TGGGCCTGAA TAAACAGAGG AAAGACTTCC TGATTGGCCT CCCTAGTGGC
    TTTGAAGAAA ACAAGGCAGT AAAGGGAATT GGCATACAAG CTGTCCCTCC CATCAGCCTA
    GTTTATAACA TGAAGCAGGT GTTTCAGCTG AGGGCCCACA TGTATCAAGC TCGCAGTCTG
    TTTGCTGCGG ACACTAGTGG CCTGTCTGAT CCATTTGCAA GGGTTTTCTT CTCCACCCAC
    AGCCAGGTCA CGGAGGTCCT CAGTGAGACT CTCTGTCCAA CATGGGATCA GCTGCTTGTG
    TTTGATAATG TTGAACTTTA CGGTGAGGCC GGTGAACTCC GTGATGACCC TCCAATCATT
    GTCATTGAGT TGTATGATCA AGACACTGTG GGGAAAGCTG AGTTTATTGG CCGAACATTT
    GCAAAACCAC TTACTAAGAT GGTTGATGAA CACTACGGCC CACCGCGGTT TCCTCCCCAG
    CTCGAGTATT ATCAGATCTA CCGAGGAAAC TGTGCTGCGG GAGATCTATT GGCTGCTTTT
    GAGCTTCTAC AGATTGGTCC AGCAGGGCGG GCAGCACTTC CTCCAATCGA TGGACCGACC
    GATTCTGACC GTGGGCCCAT TCTGCCTGTT CCATTGGGAA TTCGACCAGT TCTCAGCAGA
    TATCGCATCG AGGTCCTGTT TTGGGGTCTG AGGGACCTTA AAAGGGTCAA CCTGGCTCAG
    GTGGACCGAC CTCGTGTGGA CATTGAGTGT GCAGGGAAAG GAGTTCAGTC AGCCCTAATT
    CAAAACTACA AGAAGAATCC AAACTTCAGC ACATTAGTAA AGTGGTTTGA AGTGGATCTG
    CCAGAAAATG AGCTGCTCCA TCCACCACTT AATATTCGGG TAGTGGATTG CAGGGCATTC
    GGACGCTACA TTTTGGTGGG GTCTCATGCT GTGACCACCC TTCGCAAGTT CATCTACAGC
    CCCCCAGACA AGACTGCTAA CAACTGGGCT CACACAGCTA GACTGGCCAA TGGCTACCTG
    GCTCTCAGCA ATGGGACGTC TCATTCCCGC CCCCAATCCC GTCCTGTTTC TTATCCCTCA
    GGTGACATTG TGGTCAACAT GAGCCCTGAA CCTAACATTA AGAAGATGGA CACGGTTGTC
    AAGATTGAAG CTACCACCGA TGCGGTTGTT AAAGTTGACT TGAATGAGGA TGAGAAGGAG
    AAAGAAAAGA AGAAGAAGAA GAAGAAGAAA GGAGAAGAAG TGGAGGAAGA GGAGCCGGAT
    GAGAGCATGC TGGACTGGTG GTCCAAATAC TTTGCCTCAA TAGAGACTAT GATGGAGAAT
    CTAAGAGCTC AGGAGGCCGC TCAGGCAGAG GCAGAGGAAA GAGAAGATTT GGAGATTGCA
    GCAGAGAGTG CAGAGATCAA AGCTGATGAC TTTCCTATGA AAGGCACCAA ACCTAAAGAG
    AAGAGCAAAG ACAAGAAGAG CACCAAGGAC AAAAAGAAGA ACAACGATGG CACAGAAAAA
    CGACCTCCAA AACCAAAAGT TGATGAACTC ATGGTGTACA ATAAAGAGCT GGAGAGTGAG
    TTCGGTAGCT TTGAGGACTG GCTTCACACC TTCAACCTGT ACCGAGGAAA GGCTGGAGAT
    GATGATGACC ACAATGTGGT TGATGAGGAC AGAATTGTGG GCAGATTCAA GGGCTCCCTC
    TGTATGTATA AACTACCACT GTCTGAGGAG ATCACCAGAG AGGCAGGATT TGATCCAAAC
    ATGGGCATGT TCCAGAGTAT TCCTCACAAT GACCCTATCA ACGTTCTTGT CAGGATTTAT
    ATCATTCGAG CCACAGACCT GCATCCTGCA GATATAAATG GAAAGGCCGA CCCTTATATA
    GTCATTAAAT TGGGAAAGTC AGACATTCGA GACAAGGAGA ACTACATATC CAAGCAGCTG
    AATCCTGTCT TTGGAAAGTC ATTTGATATA GAGGCCACGT TCCCAATGGA GTCTATGCTG
    ACTGTGGCAG TGTATGACTG GGATTTAGTC GGAACTGATG ACCTGATTGG TGAGACTAAG
    ATTGATTTGG AGAACAGATA CTACAGCAAA CACAGAGCCA CATGTGGCAT TGCTTCCAAC
    TACTCTGTCC ATGGTTATAA TGTATGGAGA GATCCTCAGA AACCAGCTCA GATCCTGGCT
    AAGTTGTGCA AAGAAGGTAA ACTGGATGGA CCTCACTATG GACCTGGGGG AAGAGTCAAA
    GTTGCAAACC GGATCTTTCT TGGGCCGACA GAAATTGAGG ATGAGAGTGG TCTGAAAAAA
    CAAACAGAAG AACACTTGGC GCTCACAGTC CTTAGACATT GGGAGGAAAT CCCACGTGTT
    GGTTGCAAAC TCATCCCTGA GCATGTGGAG ACGAGGCCTC TCCTCAACCC AGACAAGCCA
    GGCATAGAGC AGGGAAGGAT TGAAATGTGG GTGGACATGT TTCCTATGGA TGTCCCAGCA
    CCAGGACCAG CCATTGATAT ATCGCCACGC AAACCAAAGA GATATGAGCT CAGGGTGATA
    ATATGGAATA CTGACGAGGT AATTCTGGAA GACGATGATT ACTTCACAGG GGAAAAGTCC
    AGTGACATTT TTGTGAGGGG CTGGCTTAAA GGACAGCAGG AGGACAAACA GGACACCGAT
    GTACATTATC ACTCTCTAAC TGGAGAGGGA AACTTCAACT GGCGCTTTGT CTTCCCCTTT
    GACTATCTCA TGGCCGAGGA GAAGATTGTT ATCTCCAAGA AGGAATCAAT GTTTTCCTGG
    GATGAGACTG AGTATAAGAT CCCTGCTCGA CTCACCCTTC AAGTATGGGA TGCCGATCAC
    TTTTCTGCAG ATGATTTTCT GGGTGCGATT GAGTTGGACT TGAATAAGTT TCCGCGTGGG
    GCAAAAACAG CCAAGCAGTG CTCTCTAGAC ATGGTTCTCA AAGAGCATGA GCTGCCAACC
    ATCTCCATCT TCAAACAAAA GAGAGTCAAG GGCTGGTGGC CATTTGTGGC CCAAAATGAG
    AATGATGAAT TTGAGCTAAC GGGAAAAGTA GAGGCAGAGC TCCATCTGTT GACAGCAGAG
    GAGGCAGAGA AGAGTCCAGT CGGCCTGGGG AGGAATGATC CGGAGCCACT TGAGAAACCA
    AATCGTCCAG ACACCAGTCT AATGTGGTTC ATGAACCCTC TGAGGTCCAT ACGATACTTC
    ATTTGGCACA ACTACCGCTG GCTGATCCTG AAGGCTCTGG CTCTGCTGCT GCTCCTTCTC
    CTCGTTGGGC TCTTCCTCTA CTCTATTCCT GGATACCTGG TCAAGAAACT CCTAGGGGCT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42615
    Clone ID Related Accession (Same CDS sequence) XM_009294744.3 , XM_009294744.2
    Accession Version XM_009294744.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5979bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product otoferlin isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009294744.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGCTTTAG TGGTGCATCT GAAGACTGTC ACGGAGCTCC GTGGGAAGGG GGACCGAATC 
    GCTAAAGTCA CTTTCAGAGG TCTCTCCTTC TTCTCTCGGG TGTTGGAAAA CTGCGAGGAT
    GAAGCTCGAT TTGAGCAGGC GTTCCGATGG CCAATTGGGA GTCAAGTGGA TGGCGACGAG
    ATGCTGGAGA TCCAAGTGTT TAATTACAGC AAAGTCTTCA CTAATAGGTT AATCGGCACA
    TTCCGCATGG TTCTTCAGAA GGTGGTGGAG GAGGGACACC TGGAGGTGTC TGACACACTC
    ATTGACGACA ATAACACAGC CATACAGACA ACCATTTCCA TAGAGATCAA ATATCAGACC
    ATGGACGGGA GTGTAAAGGT GTGGAGCGAT GGTGAATTCC TTGATATTCC AGATGACCTT
    GATGGAACCT TCCAGTTTGA GACAGATAGT CTTCTCTCAG GACGCAGCCA GAGTTCTGGA
    ACTTCACCTG GACGATCCAT TCATGGCATT CCCACATTCC GCAAGGCAGG AAAAGGAGTG
    TTCTCAGCCA TGAAGCTGGG CAAGACCCGC ACCTCTAAAG ATGACCACAA AAAAGGAGAT
    GATGCTGCCA TTCTGGATGC AGAAGATCTG GATCGAAAAG CCATGCGTCT AGGCGGCATT
    CTTGACCCTG ATACAATCTC ACTGGCCTCG GTTACTGCAG TGACCACTAA TGTCTCCAAC
    AAAAGATCAA AGCCTGACAT TAAAATGGAG CCGAGTTCAG GGCGTCCGGT TGATTATCAG
    ATCAGTGTTA CAGTAATCGA GGCCCGTCAG CTGGTCGGAT TGAACATGGA CCCTGTGGTT
    TGCGTGGAAA TTGGAGAGGA GAAGAAGTAC ACGTCAATGA AGGAGTCAAC AAACTGCCCT
    TACTACAATG AGTATTTTGT CTTCGACTTC CACGTGCCTC CAGACGTCAT GTTTGACAAA
    ATTATAAAGA TTTCAGTAAT ACATTCTAAA AACCTTCTGC GAAGCGGCAC CCTCGTGGGC
    ACCTTCAAAC TAGACGTGGG GACTGTTTAC ACACAACCTG AACATCAGTT TCATCACAAA
    TGGGCGATGC TGTCAGACCC TGATGACATC ACGACTGGCT GCAAAGGTTA TGTTAAATGT
    GACATCGCAG TCGTGGGAAA AGGAGACAAC ATCAAGACTC CACACAAGGC CAGTGAAGCA
    GACGAGGATG ACATAGAGGG GAATCTTCTC TTGCCGGAGG GTGTTCCATC AGAGAGACAG
    TGGGCTCGGT TTTATGTTAA GATTTATAGA GCTGAGGGTC TGCCTAAAAT GAACACCAGT
    ATCATGGCCA ACGTGAAGAA AGCCTTTATT GGGGAAAACA GGGACCTTGT GGACCCTTAC
    GTCCTGGTGC AATTTGCAGG GCAAAAGGGC AAAACATCTG TTCAGAAGAG CAGCTACGAG
    CCCATCTGGA ACGAGCAAGT CATCTTCACG GAGATGTTCC CACCTTTGTG TAGGCGACTG
    AAAGTTCAGA TCCGTGATTC AGACAAGGTG AACGATGTTG CCATAGGAAC CCACTTCATT
    GATCTACGAA AGGTTTCCAA TGATGGAGAC AAAGGTTTCT TGCCCACTAT GGGTCCGGCT
    TGGGTGAATA TGTATGGGTC GACCCGTAAC TACACCCTGA TGGACGAACA CCAGGACCTG
    AATGAGGGGT TGGGGGAAGG GGTGTCCTTC AGAGCACGTC TGCTCATTAG TATCGCGGTG
    GAGATTCTAG ACACCACCTC TGCTGAGATC ATGAGCTCCA CTGAAGTACA CATGGAGCCG
    GTGTCCAACA TCTCAGAGAG TGCTACAGGA AAGATGGAAG AATTTTTCCT TTTCGGGTCA
    TTCTTGGAGG CCACAATGAT AGACAGAAAA ATAGGAGATA AACCCATCAG CTTTGAAGTC
    ACCATCGGTA ACTATGGTAA CCAGATTGAT GGTGTGAGCA AGCCTGCATT AGCGAAGAAG
    AAGAAGGAGG GTGGAGGAGA GAGTGAAGAA GAGGAGTCTG AGCTTATCCA CAACTCCAGC
    GAGGAAGAGG CAGAGGATGA TGGAGATCTG ACCTCTGTAC CGTCTACACC TCCTATGAAG
    CCTGTTATCA CCGACAGAAA TTACTTCCAC TTGCCTTACT TTGAAAAGAA ACCCTGCATT
    TACATCAAGA GCTGGTGGCA GGACCAGCGG AGACGTCTCT ACAACTCCAA CATAATGGAC
    AAGATAGCTG ATAAACTGGA GGAAGGTCTG AATGATGTTC AAGAGATCAT AAAAACAGAG
    AAGGCATATC CAGAACGAAG ACTAAGAGGT GTATTAGAAG AACTTAGCAC AAGCTGCAGC
    CGGTTTGTTA CACTTGCAAA CAAGGATCAG AATCTTTCTG GAAGAACCAA GCTGGACAGA
    GAGAGGCTGA AGTCCTGTAT GAGAGAAATG GAGAGTATGG GTCAGCAGGC GAAGACCATC
    CGCACACAAG TGAAAAGAAA CACAGTTCGA GATAAACTCA AGCTAGTGTT GAATTTCCTT
    CAGAGGCTTC GATTCTTAGC AGACGAGCCC CAGCACAGCA TCCCTGATGT GTTCATATGG
    ATGATTAGCA ACAACAAACG CATCGCATAC GCCCGCATAC CCTCCAAAGA CATCCTCTAC
    TCAATTGTCG ATGAAGAGAT GGGCAAAGAC TGTGGAAAAG TTAAAGCTGT TTTTCTCAGG
    CTGCCTGGAA AGAAAGGTTT TGGGCCTGCT GGCTGGACAG TTCAGGCTAA ATTAGAGATG
    TATTTGTGGC TGGGCCTGAA TAAACAGAGG AAAGACTTCC TGATTGGCCT CCCTAGTGGC
    TTTGAAGAAA ACAAGGCAGT AAAGGGAATT GGCATACAAG CTGTCCCTCC CATCAGCCTA
    GTTTATAACA TGAAGCAGGT GTTTCAGCTG AGGGCCCACA TGTATCAAGC TCGCAGTCTG
    TTTGCTGCGG ACACTAGTGG CCTGTCTGAT CCATTTGCAA GGGTTTTCTT CTCCACCCAC
    AGCCAGGTCA CGGAGGTCCT CAGTGAGACT CTCTGTCCAA CATGGGATCA GCTGCTTGTG
    TTTGATAATG TTGAACTTTA CGGTGAGGCC GGTGAACTCC GTGATGACCC TCCAATCATT
    GTCATTGAGT TGTATGATCA AGACACTGTG GGGAAAGCTG AGTTTATTGG CCGAACATTT
    GCAAAACCAC TTACTAAGAT GGTTGATGAA CACTACGGCC CACCGCGGTT TCCTCCCCAG
    CTCGAGTATT ATCAGATCTA CCGAGGAAAC TGTGCTGCGG GAGATCTATT GGCTGCTTTT
    GAGCTTCTAC AGATTGGTCC AGCAGGGCGG GCAGCACTTC CTCCAATCGA TGGACCGACC
    GATTCTGACC GTGGGCCCAT TCTGCCTGTT CCATTGGGAA TTCGACCAGT TCTCAGCAGA
    TATCGCATCG AGGTCCTGTT TTGGGGTCTG AGGGACCTTA AAAGGGTCAA CCTGGCTCAG
    GTGGACCGAC CTCGTGTGGA CATTGAGTGT GCAGGGAAAG GAGTTCAGTC AGCCCTAATT
    CAAAACTACA AGAAGAATCC AAACTTCAGC ACATTAGTAA AGTGGTTTGA AGTGGATCTG
    CCAGAAAATG AGCTGCTCCA TCCACCACTT AATATTCGGG TAGTGGATTG CAGGGCATTC
    GGACGCTACA TTTTGGTGGG GTCTCATGCT GTGACCACCC TTCGCAAGTT CATCTACAGC
    CCCCCAGACA AGACTGCTAA CAACTGGGCT CACACAGGTG ACATTGTGGT CAACATGAGC
    CCTGAACCTA ACATTAAGAA GATGGACACG GTTGTCAAGA TTGAAGCTAC CACCGATGCG
    GTTGTTAAAG TTGACTTGAA TGAGGATGAG AAGGAGAAAG AAAAGAAGAA GAAGAAGAAG
    AAGAAAGGAG AAGAAGTGGA GGAAGAGGAG CCGGATGAGA GCATGCTGGA CTGGTGGTCC
    AAATACTTTG CCTCAATAGA GACTATGATG GAGAATCTAA GAGCTCAGGA GGCCGCTCAG
    GCAGAGGCAG AGGAAAGAGA AGATTTGGAG ATTGCAGCAG AGAGTGCAGA GATCAAAGCT
    GATGACTTTC CTATGAAAGG CACCAAACCT AAAGAGAAGA GCAAAGACAA GAAGAGCACC
    AAGGACAAAA AGAAGAACAA CGATGGCACA GAAAAACGAC CTCCAAAACC AAAAGTTGAT
    GAACTCATGG TGTACAATAA AGAGCTGGAG AGTGAGTTCG GTAGCTTTGA GGACTGGCTT
    CACACCTTCA ACCTGTACCG AGGAAAGGCT GGAGATGATG ATGACCACAA TGTGGTTGAT
    GAGGACAGAA TTGTGGGCAG ATTCAAGGGC TCCCTCTGTA TGTATAAACT ACCACTGTCT
    GAGGAGATCA CCAGAGAGGC AGGATTTGAT CCAAACATGG GCATGTTCCA GAGTATTCCT
    CACAATGACC CTATCAACGT TCTTGTCAGG ATTTATATCA TTCGAGCCAC AGACCTGCAT
    CCTGCAGATA TAAATGGAAA GGCCGACCCT TATATAGTCA TTAAATTGGG AAAGTCAGAC
    ATTCGAGACA AGGAGAACTA CATATCCAAG CAGCTGAATC CTGTCTTTGG AAAGTCATTT
    GATATAGAGG CCACGTTCCC AATGGAGTCT ATGCTGACTG TGGCAGTGTA TGACTGGGAT
    TTAGTCGGAA CTGATGACCT GATTGGTGAG ACTAAGATTG ATTTGGAGAA CAGATACTAC
    AGCAAACACA GAGCCACATG TGGCATTGCT TCCAACTACT CTGTCCATGG TTATAATGTA
    TGGAGAGATC CTCAGAAACC AGCTCAGATC CTGGCTAAGT TGTGCAAAGA AGGTAAACTG
    GATGGACCTC ACTATGGACC TGGGGGAAGA GTCAAAGTTG CAAACCGGAT CTTTCTTGGG
    CCGACAGAAA TTGAGGATGA GAGTGGTCTG AAAAAACAAA CAGAAGAACA CTTGGCGCTC
    ACAGTCCTTA GACATTGGGA GGAAATCCCA CGTGTTGGTT GCAAACTCAT CCCTGAGCAT
    GTGGAGACGA GGCCTCTCCT CAACCCAGAC AAGCCAGGCA TAGAGCAGGG AAGGATTGAA
    ATGTGGGTGG ACATGTTTCC TATGGATGTC CCAGCACCAG GACCAGCCAT TGATATATCG
    CCACGCAAAC CAAAGAGATA TGAGCTCAGG GTGATAATAT GGAATACTGA CGAGGTAATT
    CTGGAAGACG ATGATTACTT CACAGGGGAA AAGTCCAGTG ACATTTTTGT GAGGGGCTGG
    CTTAAAGGAC AGCAGGAGGA CAAACAGGAC ACCGATGTAC ATTATCACTC TCTAACTGGA
    GAGGGAAACT TCAACTGGCG CTTTGTCTTC CCCTTTGACT ATCTCATGGC CGAGGAGAAG
    ATTGTTATCT CCAAGAAGGA ATCAATGTTT TCCTGGGATG AGACTGAGTA TAAGATCCCT
    GCTCGACTCA CCCTTCAAGT ATGGGATGCC GATCACTTTT CTGCAGATGA TTTTCTGGGT
    GCGATTGAGT TGGACTTGAA TAAGTTTCCG CGTGGGGCAA AAACAGCCAA GCAGTGCTCT
    CTAGACATGG TTCTCAAAGA GCATGAGCTG CCAACCATCT CCATCTTCAA ACAAAAGAGA
    GTCAAGGGCT GGTGGCCATT TGTGGCCCAA AATGAGAATG ATGAATTTGA GCTAACGGGA
    AAAGTAGAGG CAGAGCTCCA TCTGTTGACA GCAGAGGAGG CAGAGAAGAG TCCAGTCGGC
    CTGGGGAGGA ATGATCCGGA GCCACTTGAG AAACCAAACC GGCCCGACAC CACCTTCCTG
    TGGTTTCTGA GCCCACTGAA GGCCATCCGC TACCTGATAT GCAACCGCTA CAAGTGGCTC
    ATCATCAAGA TTGTGTTGGC TCTGCTGCTG CTGATCATGG TGGGCCTGTT TCTCTACAGC
    ATGCCAGGCT ATCTGGTCAA GAAGCTGCTC GGGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009293019.1
    CDS300..6278
    Translation

    Target ORF information:

    RefSeq Version XM_009294744.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio otoferlin a (otofa), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009294744.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGCTTTAG TGGTGCATCT GAAGACTGTC ACGGAGCTCC GTGGGAAGGG GGACCGAATC 
    GCTAAAGTCA CTTTCAGAGG TCTCTCCTTC TTCTCTCGGG TGTTGGAAAA CTGCGAGGAT
    GAAGCTCGAT TTGAGCAGGC GTTCCGATGG CCAATTGGGA GTCAAGTGGA TGGCGACGAG
    ATGCTGGAGA TCCAAGTGTT TAATTACAGC AAAGTCTTCA CTAATAGGTT AATCGGCACA
    TTCCGCATGG TTCTTCAGAA GGTGGTGGAG GAGGGACACC TGGAGGTGTC TGACACACTC
    ATTGACGACA ATAACACAGC CATACAGACA ACCATTTCCA TAGAGATCAA ATATCAGACC
    ATGGACGGGA GTGTAAAGGT GTGGAGCGAT GGTGAATTCC TTGATATTCC AGATGACCTT
    GATGGAACCT TCCAGTTTGA GACAGATAGT CTTCTCTCAG GACGCAGCCA GAGTTCTGGA
    ACTTCACCTG GACGATCCAT TCATGGCATT CCCACATTCC GCAAGGCAGG AAAAGGAGTG
    TTCTCAGCCA TGAAGCTGGG CAAGACCCGC ACCTCTAAAG ATGACCACAA AAAAGGAGAT
    GATGCTGCCA TTCTGGATGC AGAAGATCTG GATCGAAAAG CCATGCGTCT AGGCGGCATT
    CTTGACCCTG ATACAATCTC ACTGGCCTCG GTTACTGCAG TGACCACTAA TGTCTCCAAC
    AAAAGATCAA AGCCTGACAT TAAAATGGAG CCGAGTTCAG GGCGTCCGGT TGATTATCAG
    ATCAGTGTTA CAGTAATCGA GGCCCGTCAG CTGGTCGGAT TGAACATGGA CCCTGTGGTT
    TGCGTGGAAA TTGGAGAGGA GAAGAAGTAC ACGTCAATGA AGGAGTCAAC AAACTGCCCT
    TACTACAATG AGTATTTTGT CTTCGACTTC CACGTGCCTC CAGACGTCAT GTTTGACAAA
    ATTATAAAGA TTTCAGTAAT ACATTCTAAA AACCTTCTGC GAAGCGGCAC CCTCGTGGGC
    ACCTTCAAAC TAGACGTGGG GACTGTTTAC ACACAACCTG AACATCAGTT TCATCACAAA
    TGGGCGATGC TGTCAGACCC TGATGACATC ACGACTGGCT GCAAAGGTTA TGTTAAATGT
    GACATCGCAG TCGTGGGAAA AGGAGACAAC ATCAAGACTC CACACAAGGC CAGTGAAGCA
    GACGAGGATG ACATAGAGGG GAATCTTCTC TTGCCGGAGG GTGTTCCATC AGAGAGACAG
    TGGGCTCGGT TTTATGTTAA GATTTATAGA GCTGAGGGTC TGCCTAAAAT GAACACCAGT
    ATCATGGCCA ACGTGAAGAA AGCCTTTATT GGGGAAAACA GGGACCTTGT GGACCCTTAC
    GTCCTGGTGC AATTTGCAGG GCAAAAGGGC AAAACATCTG TTCAGAAGAG CAGCTACGAG
    CCCATCTGGA ACGAGCAAGT CATCTTCACG GAGATGTTCC CACCTTTGTG TAGGCGACTG
    AAAGTTCAGA TCCGTGATTC AGACAAGGTG AACGATGTTG CCATAGGAAC CCACTTCATT
    GATCTACGAA AGGTTTCCAA TGATGGAGAC AAAGGTTTCT TGCCCACTAT GGGTCCGGCT
    TGGGTGAATA TGTATGGGTC GACCCGTAAC TACACCCTGA TGGACGAACA CCAGGACCTG
    AATGAGGGGT TGGGGGAAGG GGTGTCCTTC AGAGCACGTC TGCTCATTAG TATCGCGGTG
    GAGATTCTAG ACACCACCTC TGCTGAGATC ATGAGCTCCA CTGAAGTACA CATGGAGCCG
    GTGTCCAACA TCTCAGAGAG TGCTACAGGA AAGATGGAAG AATTTTTCCT TTTCGGGTCA
    TTCTTGGAGG CCACAATGAT AGACAGAAAA ATAGGAGATA AACCCATCAG CTTTGAAGTC
    ACCATCGGTA ACTATGGTAA CCAGATTGAT GGTGTGAGCA AGCCTGCATT AGCGAAGAAG
    AAGAAGGAGG GTGGAGGAGA GAGTGAAGAA GAGGAGTCTG AGCTTATCCA CAACTCCAGC
    GAGGAAGAGG CAGAGGATGA TGGAGATCTG ACCTCTGTAC CGTCTACACC TCCTATGAAG
    CCTGTTATCA CCGACAGAAA TTACTTCCAC TTGCCTTACT TTGAAAAGAA ACCCTGCATT
    TACATCAAGA GCTGGTGGCA GGACCAGCGG AGACGTCTCT ACAACTCCAA CATAATGGAC
    AAGATAGCTG ATAAACTGGA GGAAGGTCTG AATGATGTTC AAGAGATCAT AAAAACAGAG
    AAGGCATATC CAGAACGAAG ACTAAGAGGT GTATTAGAAG AACTTAGCAC AAGCTGCAGC
    CGGTTTGTTA CACTTGCAAA CAAGGATCAG AATCTTTCTG GAAGAACCAA GCTGGACAGA
    GAGAGGCTGA AGTCCTGTAT GAGAGAAATG GAGAGTATGG GTCAGCAGGC GAAGACCATC
    CGCACACAAG TGAAAAGAAA CACAGTTCGA GATAAACTCA AGCTAGTGTT GAATTTCCTT
    CAGAGGCTTC GATTCTTAGC AGACGAGCCC CAGCACAGCA TCCCTGATGT GTTCATATGG
    ATGATTAGCA ACAACAAACG CATCGCATAC GCCCGCATAC CCTCCAAAGA CATCCTCTAC
    TCAATTGTCG ATGAAGAGAT GGGCAAAGAC TGTGGAAAAG TTAAAGCTGT TTTTCTCAGG
    CTGCCTGGAA AGAAAGGTTT TGGGCCTGCT GGCTGGACAG TTCAGGCTAA ATTAGAGATG
    TATTTGTGGC TGGGCCTGAA TAAACAGAGG AAAGACTTCC TGATTGGCCT CCCTAGTGGC
    TTTGAAGAAA ACAAGGCAGT AAAGGGAATT GGCATACAAG CTGTCCCTCC CATCAGCCTA
    GTTTATAACA TGAAGCAGGT GTTTCAGCTG AGGGCCCACA TGTATCAAGC TCGCAGTCTG
    TTTGCTGCGG ACACTAGTGG CCTGTCTGAT CCATTTGCAA GGGTTTTCTT CTCCACCCAC
    AGCCAGGTCA CGGAGGTCCT CAGTGAGACT CTCTGTCCAA CATGGGATCA GCTGCTTGTG
    TTTGATAATG TTGAACTTTA CGGTGAGGCC GGTGAACTCC GTGATGACCC TCCAATCATT
    GTCATTGAGT TGTATGATCA AGACACTGTG GGGAAAGCTG AGTTTATTGG CCGAACATTT
    GCAAAACCAC TTACTAAGAT GGTTGATGAA CACTACGGCC CACCGCGGTT TCCTCCCCAG
    CTCGAGTATT ATCAGATCTA CCGAGGAAAC TGTGCTGCGG GAGATCTATT GGCTGCTTTT
    GAGCTTCTAC AGATTGGTCC AGCAGGGCGG GCAGCACTTC CTCCAATCGA TGGACCGACC
    GATTCTGACC GTGGGCCCAT TCTGCCTGTT CCATTGGGAA TTCGACCAGT TCTCAGCAGA
    TATCGCATCG AGGTCCTGTT TTGGGGTCTG AGGGACCTTA AAAGGGTCAA CCTGGCTCAG
    GTGGACCGAC CTCGTGTGGA CATTGAGTGT GCAGGGAAAG GAGTTCAGTC AGCCCTAATT
    CAAAACTACA AGAAGAATCC AAACTTCAGC ACATTAGTAA AGTGGTTTGA AGTGGATCTG
    CCAGAAAATG AGCTGCTCCA TCCACCACTT AATATTCGGG TAGTGGATTG CAGGGCATTC
    GGACGCTACA TTTTGGTGGG GTCTCATGCT GTGACCACCC TTCGCAAGTT CATCTACAGC
    CCCCCAGACA AGACTGCTAA CAACTGGGCT CACACAGGTG ACATTGTGGT CAACATGAGC
    CCTGAACCTA ACATTAAGAA GATGGACACG GTTGTCAAGA TTGAAGCTAC CACCGATGCG
    GTTGTTAAAG TTGACTTGAA TGAGGATGAG AAGGAGAAAG AAAAGAAGAA GAAGAAGAAG
    AAGAAAGGAG AAGAAGTGGA GGAAGAGGAG CCGGATGAGA GCATGCTGGA CTGGTGGTCC
    AAATACTTTG CCTCAATAGA GACTATGATG GAGAATCTAA GAGCTCAGGA GGCCGCTCAG
    GCAGAGGCAG AGGAAAGAGA AGATTTGGAG ATTGCAGCAG AGAGTGCAGA GATCAAAGCT
    GATGACTTTC CTATGAAAGG CACCAAACCT AAAGAGAAGA GCAAAGACAA GAAGAGCACC
    AAGGACAAAA AGAAGAACAA CGATGGCACA GAAAAACGAC CTCCAAAACC AAAAGTTGAT
    GAACTCATGG TGTACAATAA AGAGCTGGAG AGTGAGTTCG GTAGCTTTGA GGACTGGCTT
    CACACCTTCA ACCTGTACCG AGGAAAGGCT GGAGATGATG ATGACCACAA TGTGGTTGAT
    GAGGACAGAA TTGTGGGCAG ATTCAAGGGC TCCCTCTGTA TGTATAAACT ACCACTGTCT
    GAGGAGATCA CCAGAGAGGC AGGATTTGAT CCAAACATGG GCATGTTCCA GAGTATTCCT
    CACAATGACC CTATCAACGT TCTTGTCAGG ATTTATATCA TTCGAGCCAC AGACCTGCAT
    CCTGCAGATA TAAATGGAAA GGCCGACCCT TATATAGTCA TTAAATTGGG AAAGTCAGAC
    ATTCGAGACA AGGAGAACTA CATATCCAAG CAGCTGAATC CTGTCTTTGG AAAGTCATTT
    GATATAGAGG CCACGTTCCC AATGGAGTCT ATGCTGACTG TGGCAGTGTA TGACTGGGAT
    TTAGTCGGAA CTGATGACCT GATTGGTGAG ACTAAGATTG ATTTGGAGAA CAGATACTAC
    AGCAAACACA GAGCCACATG TGGCATTGCT TCCAACTACT CTGTCCATGG TTATAATGTA
    TGGAGAGATC CTCAGAAACC AGCTCAGATC CTGGCTAAGT TGTGCAAAGA AGGTAAACTG
    GATGGACCTC ACTATGGACC TGGGGGAAGA GTCAAAGTTG CAAACCGGAT CTTTCTTGGG
    CCGACAGAAA TTGAGGATGA GAGTGGTCTG AAAAAACAAA CAGAAGAACA CTTGGCGCTC
    ACAGTCCTTA GACATTGGGA GGAAATCCCA CGTGTTGGTT GCAAACTCAT CCCTGAGCAT
    GTGGAGACGA GGCCTCTCCT CAACCCAGAC AAGCCAGGCA TAGAGCAGGG AAGGATTGAA
    ATGTGGGTGG ACATGTTTCC TATGGATGTC CCAGCACCAG GACCAGCCAT TGATATATCG
    CCACGCAAAC CAAAGAGATA TGAGCTCAGG GTGATAATAT GGAATACTGA CGAGGTAATT
    CTGGAAGACG ATGATTACTT CACAGGGGAA AAGTCCAGTG ACATTTTTGT GAGGGGCTGG
    CTTAAAGGAC AGCAGGAGGA CAAACAGGAC ACCGATGTAC ATTATCACTC TCTAACTGGA
    GAGGGAAACT TCAACTGGCG CTTTGTCTTC CCCTTTGACT ATCTCATGGC CGAGGAGAAG
    ATTGTTATCT CCAAGAAGGA ATCAATGTTT TCCTGGGATG AGACTGAGTA TAAGATCCCT
    GCTCGACTCA CCCTTCAAGT ATGGGATGCC GATCACTTTT CTGCAGATGA TTTTCTGGGT
    GCGATTGAGT TGGACTTGAA TAAGTTTCCG CGTGGGGCAA AAACAGCCAA GCAGTGCTCT
    CTAGACATGG TTCTCAAAGA GCATGAGCTG CCAACCATCT CCATCTTCAA ACAAAAGAGA
    GTCAAGGGCT GGTGGCCATT TGTGGCCCAA AATGAGAATG ATGAATTTGA GCTAACGGGA
    AAAGTAGAGG CAGAGCTCCA TCTGTTGACA GCAGAGGAGG CAGAGAAGAG TCCAGTCGGC
    CTGGGGAGGA ATGATCCGGA GCCACTTGAG AAACCAAACC GGCCCGACAC CACCTTCCTG
    TGGTTTCTGA GCCCACTGAA GGCCATCCGC TACCTGATAT GCAACCGCTA CAAGTGGCTC
    ATCATCAAGA TTGTGTTGGC TCTGCTGCTG CTGATCATGG TGGGCCTGTT TCTCTACAGC
    ATGCCAGGCT ATCTGGTCAA GAAGCTGCTC GGGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42615
    Clone ID Related Accession (Same CDS sequence) XM_009294744.3 , XM_009294744.2
    Accession Version XM_009294744.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5979bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product otoferlin isoform X10
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009294744.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGCTTTAG TGGTGCATCT GAAGACTGTC ACGGAGCTCC GTGGGAAGGG GGACCGAATC 
    GCTAAAGTCA CTTTCAGAGG TCTCTCCTTC TTCTCTCGGG TGTTGGAAAA CTGCGAGGAT
    GAAGCTCGAT TTGAGCAGGC GTTCCGATGG CCAATTGGGA GTCAAGTGGA TGGCGACGAG
    ATGCTGGAGA TCCAAGTGTT TAATTACAGC AAAGTCTTCA CTAATAGGTT AATCGGCACA
    TTCCGCATGG TTCTTCAGAA GGTGGTGGAG GAGGGACACC TGGAGGTGTC TGACACACTC
    ATTGACGACA ATAACACAGC CATACAGACA ACCATTTCCA TAGAGATCAA ATATCAGACC
    ATGGACGGGA GTGTAAAGGT GTGGAGCGAT GGTGAATTCC TTGATATTCC AGATGACCTT
    GATGGAACCT TCCAGTTTGA GACAGATAGT CTTCTCTCAG GACGCAGCCA GAGTTCTGGA
    ACTTCACCTG GACGATCCAT TCATGGCATT CCCACATTCC GCAAGGCAGG AAAAGGAGTG
    TTCTCAGCCA TGAAGCTGGG CAAGACCCGC ACCTCTAAAG ATGACCACAA AAAAGGAGAT
    GATGCTGCCA TTCTGGATGC AGAAGATCTG GATCGAAAAG CCATGCGTCT AGGCGGCATT
    CTTGACCCTG ATACAATCTC ACTGGCCTCG GTTACTGCAG TGACCACTAA TGTCTCCAAC
    AAAAGATCAA AGCCTGACAT TAAAATGGAG CCGAGTTCAG GGCGTCCGGT TGATTATCAG
    ATCAGTGTTA CAGTAATCGA GGCCCGTCAG CTGGTCGGAT TGAACATGGA CCCTGTGGTT
    TGCGTGGAAA TTGGAGAGGA GAAGAAGTAC ACGTCAATGA AGGAGTCAAC AAACTGCCCT
    TACTACAATG AGTATTTTGT CTTCGACTTC CACGTGCCTC CAGACGTCAT GTTTGACAAA
    ATTATAAAGA TTTCAGTAAT ACATTCTAAA AACCTTCTGC GAAGCGGCAC CCTCGTGGGC
    ACCTTCAAAC TAGACGTGGG GACTGTTTAC ACACAACCTG AACATCAGTT TCATCACAAA
    TGGGCGATGC TGTCAGACCC TGATGACATC ACGACTGGCT GCAAAGGTTA TGTTAAATGT
    GACATCGCAG TCGTGGGAAA AGGAGACAAC ATCAAGACTC CACACAAGGC CAGTGAAGCA
    GACGAGGATG ACATAGAGGG GAATCTTCTC TTGCCGGAGG GTGTTCCATC AGAGAGACAG
    TGGGCTCGGT TTTATGTTAA GATTTATAGA GCTGAGGGTC TGCCTAAAAT GAACACCAGT
    ATCATGGCCA ACGTGAAGAA AGCCTTTATT GGGGAAAACA GGGACCTTGT GGACCCTTAC
    GTCCTGGTGC AATTTGCAGG GCAAAAGGGC AAAACATCTG TTCAGAAGAG CAGCTACGAG
    CCCATCTGGA ACGAGCAAGT CATCTTCACG GAGATGTTCC CACCTTTGTG TAGGCGACTG
    AAAGTTCAGA TCCGTGATTC AGACAAGGTG AACGATGTTG CCATAGGAAC CCACTTCATT
    GATCTACGAA AGGTTTCCAA TGATGGAGAC AAAGGTTTCT TGCCCACTAT GGGTCCGGCT
    TGGGTGAATA TGTATGGGTC GACCCGTAAC TACACCCTGA TGGACGAACA CCAGGACCTG
    AATGAGGGGT TGGGGGAAGG GGTGTCCTTC AGAGCACGTC TGCTCATTAG TATCGCGGTG
    GAGATTCTAG ACACCACCTC TGCTGAGATC ATGAGCTCCA CTGAAGTACA CATGGAGCCG
    GTGTCCAACA TCTCAGAGAG TGCTACAGGA AAGATGGAAG AATTTTTCCT TTTCGGGTCA
    TTCTTGGAGG CCACAATGAT AGACAGAAAA ATAGGAGATA AACCCATCAG CTTTGAAGTC
    ACCATCGGTA ACTATGGTAA CCAGATTGAT GGTGTGAGCA AGCCTGCATT AGCGAAGAAG
    AAGAAGGAGG GTGGAGGAGA GAGTGAAGAA GAGGAGTCTG AGCTTATCCA CAACTCCAGC
    GAGGAAGAGG CAGAGGATGA TGGAGATCTG ACCTCTGTAC CGTCTACACC TCCTATGAAG
    CCTGTTATCA CCGACAGAAA TTACTTCCAC TTGCCTTACT TTGAAAAGAA ACCCTGCATT
    TACATCAAGA GCTGGTGGCA GGACCAGCGG AGACGTCTCT ACAACTCCAA CATAATGGAC
    AAGATAGCTG ATAAACTGGA GGAAGGTCTG AATGATGTTC AAGAGATCAT AAAAACAGAG
    AAGGCATATC CAGAACGAAG ACTAAGAGGT GTATTAGAAG AACTTAGCAC AAGCTGCAGC
    CGGTTTGTTA CACTTGCAAA CAAGGATCAG AATCTTTCTG GAAGAACCAA GCTGGACAGA
    GAGAGGCTGA AGTCCTGTAT GAGAGAAATG GAGAGTATGG GTCAGCAGGC GAAGACCATC
    CGCACACAAG TGAAAAGAAA CACAGTTCGA GATAAACTCA AGCTAGTGTT GAATTTCCTT
    CAGAGGCTTC GATTCTTAGC AGACGAGCCC CAGCACAGCA TCCCTGATGT GTTCATATGG
    ATGATTAGCA ACAACAAACG CATCGCATAC GCCCGCATAC CCTCCAAAGA CATCCTCTAC
    TCAATTGTCG ATGAAGAGAT GGGCAAAGAC TGTGGAAAAG TTAAAGCTGT TTTTCTCAGG
    CTGCCTGGAA AGAAAGGTTT TGGGCCTGCT GGCTGGACAG TTCAGGCTAA ATTAGAGATG
    TATTTGTGGC TGGGCCTGAA TAAACAGAGG AAAGACTTCC TGATTGGCCT CCCTAGTGGC
    TTTGAAGAAA ACAAGGCAGT AAAGGGAATT GGCATACAAG CTGTCCCTCC CATCAGCCTA
    GTTTATAACA TGAAGCAGGT GTTTCAGCTG AGGGCCCACA TGTATCAAGC TCGCAGTCTG
    TTTGCTGCGG ACACTAGTGG CCTGTCTGAT CCATTTGCAA GGGTTTTCTT CTCCACCCAC
    AGCCAGGTCA CGGAGGTCCT CAGTGAGACT CTCTGTCCAA CATGGGATCA GCTGCTTGTG
    TTTGATAATG TTGAACTTTA CGGTGAGGCC GGTGAACTCC GTGATGACCC TCCAATCATT
    GTCATTGAGT TGTATGATCA AGACACTGTG GGGAAAGCTG AGTTTATTGG CCGAACATTT
    GCAAAACCAC TTACTAAGAT GGTTGATGAA CACTACGGCC CACCGCGGTT TCCTCCCCAG
    CTCGAGTATT ATCAGATCTA CCGAGGAAAC TGTGCTGCGG GAGATCTATT GGCTGCTTTT
    GAGCTTCTAC AGATTGGTCC AGCAGGGCGG GCAGCACTTC CTCCAATCGA TGGACCGACC
    GATTCTGACC GTGGGCCCAT TCTGCCTGTT CCATTGGGAA TTCGACCAGT TCTCAGCAGA
    TATCGCATCG AGGTCCTGTT TTGGGGTCTG AGGGACCTTA AAAGGGTCAA CCTGGCTCAG
    GTGGACCGAC CTCGTGTGGA CATTGAGTGT GCAGGGAAAG GAGTTCAGTC AGCCCTAATT
    CAAAACTACA AGAAGAATCC AAACTTCAGC ACATTAGTAA AGTGGTTTGA AGTGGATCTG
    CCAGAAAATG AGCTGCTCCA TCCACCACTT AATATTCGGG TAGTGGATTG CAGGGCATTC
    GGACGCTACA TTTTGGTGGG GTCTCATGCT GTGACCACCC TTCGCAAGTT CATCTACAGC
    CCCCCAGACA AGACTGCTAA CAACTGGGCT CACACAGGTG ACATTGTGGT CAACATGAGC
    CCTGAACCTA ACATTAAGAA GATGGACACG GTTGTCAAGA TTGAAGCTAC CACCGATGCG
    GTTGTTAAAG TTGACTTGAA TGAGGATGAG AAGGAGAAAG AAAAGAAGAA GAAGAAGAAG
    AAGAAAGGAG AAGAAGTGGA GGAAGAGGAG CCGGATGAGA GCATGCTGGA CTGGTGGTCC
    AAATACTTTG CCTCAATAGA GACTATGATG GAGAATCTAA GAGCTCAGGA GGCCGCTCAG
    GCAGAGGCAG AGGAAAGAGA AGATTTGGAG ATTGCAGCAG AGAGTGCAGA GATCAAAGCT
    GATGACTTTC CTATGAAAGG CACCAAACCT AAAGAGAAGA GCAAAGACAA GAAGAGCACC
    AAGGACAAAA AGAAGAACAA CGATGGCACA GAAAAACGAC CTCCAAAACC AAAAGTTGAT
    GAACTCATGG TGTACAATAA AGAGCTGGAG AGTGAGTTCG GTAGCTTTGA GGACTGGCTT
    CACACCTTCA ACCTGTACCG AGGAAAGGCT GGAGATGATG ATGACCACAA TGTGGTTGAT
    GAGGACAGAA TTGTGGGCAG ATTCAAGGGC TCCCTCTGTA TGTATAAACT ACCACTGTCT
    GAGGAGATCA CCAGAGAGGC AGGATTTGAT CCAAACATGG GCATGTTCCA GAGTATTCCT
    CACAATGACC CTATCAACGT TCTTGTCAGG ATTTATATCA TTCGAGCCAC AGACCTGCAT
    CCTGCAGATA TAAATGGAAA GGCCGACCCT TATATAGTCA TTAAATTGGG AAAGTCAGAC
    ATTCGAGACA AGGAGAACTA CATATCCAAG CAGCTGAATC CTGTCTTTGG AAAGTCATTT
    GATATAGAGG CCACGTTCCC AATGGAGTCT ATGCTGACTG TGGCAGTGTA TGACTGGGAT
    TTAGTCGGAA CTGATGACCT GATTGGTGAG ACTAAGATTG ATTTGGAGAA CAGATACTAC
    AGCAAACACA GAGCCACATG TGGCATTGCT TCCAACTACT CTGTCCATGG TTATAATGTA
    TGGAGAGATC CTCAGAAACC AGCTCAGATC CTGGCTAAGT TGTGCAAAGA AGGTAAACTG
    GATGGACCTC ACTATGGACC TGGGGGAAGA GTCAAAGTTG CAAACCGGAT CTTTCTTGGG
    CCGACAGAAA TTGAGGATGA GAGTGGTCTG AAAAAACAAA CAGAAGAACA CTTGGCGCTC
    ACAGTCCTTA GACATTGGGA GGAAATCCCA CGTGTTGGTT GCAAACTCAT CCCTGAGCAT
    GTGGAGACGA GGCCTCTCCT CAACCCAGAC AAGCCAGGCA TAGAGCAGGG AAGGATTGAA
    ATGTGGGTGG ACATGTTTCC TATGGATGTC CCAGCACCAG GACCAGCCAT TGATATATCG
    CCACGCAAAC CAAAGAGATA TGAGCTCAGG GTGATAATAT GGAATACTGA CGAGGTAATT
    CTGGAAGACG ATGATTACTT CACAGGGGAA AAGTCCAGTG ACATTTTTGT GAGGGGCTGG
    CTTAAAGGAC AGCAGGAGGA CAAACAGGAC ACCGATGTAC ATTATCACTC TCTAACTGGA
    GAGGGAAACT TCAACTGGCG CTTTGTCTTC CCCTTTGACT ATCTCATGGC CGAGGAGAAG
    ATTGTTATCT CCAAGAAGGA ATCAATGTTT TCCTGGGATG AGACTGAGTA TAAGATCCCT
    GCTCGACTCA CCCTTCAAGT ATGGGATGCC GATCACTTTT CTGCAGATGA TTTTCTGGGT
    GCGATTGAGT TGGACTTGAA TAAGTTTCCG CGTGGGGCAA AAACAGCCAA GCAGTGCTCT
    CTAGACATGG TTCTCAAAGA GCATGAGCTG CCAACCATCT CCATCTTCAA ACAAAAGAGA
    GTCAAGGGCT GGTGGCCATT TGTGGCCCAA AATGAGAATG ATGAATTTGA GCTAACGGGA
    AAAGTAGAGG CAGAGCTCCA TCTGTTGACA GCAGAGGAGG CAGAGAAGAG TCCAGTCGGC
    CTGGGGAGGA ATGATCCGGA GCCACTTGAG AAACCAAACC GGCCCGACAC CACCTTCCTG
    TGGTTTCTGA GCCCACTGAA GGCCATCCGC TACCTGATAT GCAACCGCTA CAAGTGGCTC
    ATCATCAAGA TTGTGTTGGC TCTGCTGCTG CTGATCATGG TGGGCCTGTT TCTCTACAGC
    ATGCCAGGCT ATCTGGTCAA GAAGCTGCTC GGGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009293019.1
    CDS303..6281
    Translation

    Target ORF information:

    RefSeq Version XM_009294744.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio otoferlin a (otofa), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009294744.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGCTTTAG TGGTGCATCT GAAGACTGTC ACGGAGCTCC GTGGGAAGGG GGACCGAATC 
    GCTAAAGTCA CTTTCAGAGG TCTCTCCTTC TTCTCTCGGG TGTTGGAAAA CTGCGAGGAT
    GAAGCTCGAT TTGAGCAGGC GTTCCGATGG CCAATTGGGA GTCAAGTGGA TGGCGACGAG
    ATGCTGGAGA TCCAAGTGTT TAATTACAGC AAAGTCTTCA CTAATAGGTT AATCGGCACA
    TTCCGCATGG TTCTTCAGAA GGTGGTGGAG GAGGGACACC TGGAGGTGTC TGACACACTC
    ATTGACGACA ATAACACAGC CATACAGACA ACCATTTCCA TAGAGATCAA ATATCAGACC
    ATGGACGGGA GTGTAAAGGT GTGGAGCGAT GGTGAATTCC TTGATATTCC AGATGACCTT
    GATGGAACCT TCCAGTTTGA GACAGATAGT CTTCTCTCAG GACGCAGCCA GAGTTCTGGA
    ACTTCACCTG GACGATCCAT TCATGGCATT CCCACATTCC GCAAGGCAGG AAAAGGAGTG
    TTCTCAGCCA TGAAGCTGGG CAAGACCCGC ACCTCTAAAG ATGACCACAA AAAAGGAGAT
    GATGCTGCCA TTCTGGATGC AGAAGATCTG GATCGAAAAG CCATGCGTCT AGGCGGCATT
    CTTGACCCTG ATACAATCTC ACTGGCCTCG GTTACTGCAG TGACCACTAA TGTCTCCAAC
    AAAAGATCAA AGCCTGACAT TAAAATGGAG CCGAGTTCAG GGCGTCCGGT TGATTATCAG
    ATCAGTGTTA CAGTAATCGA GGCCCGTCAG CTGGTCGGAT TGAACATGGA CCCTGTGGTT
    TGCGTGGAAA TTGGAGAGGA GAAGAAGTAC ACGTCAATGA AGGAGTCAAC AAACTGCCCT
    TACTACAATG AGTATTTTGT CTTCGACTTC CACGTGCCTC CAGACGTCAT GTTTGACAAA
    ATTATAAAGA TTTCAGTAAT ACATTCTAAA AACCTTCTGC GAAGCGGCAC CCTCGTGGGC
    ACCTTCAAAC TAGACGTGGG GACTGTTTAC ACACAACCTG AACATCAGTT TCATCACAAA
    TGGGCGATGC TGTCAGACCC TGATGACATC ACGACTGGCT GCAAAGGTTA TGTTAAATGT
    GACATCGCAG TCGTGGGAAA AGGAGACAAC ATCAAGACTC CACACAAGGC CAGTGAAGCA
    GACGAGGATG ACATAGAGGG GAATCTTCTC TTGCCGGAGG GTGTTCCATC AGAGAGACAG
    TGGGCTCGGT TTTATGTTAA GATTTATAGA GCTGAGGGTC TGCCTAAAAT GAACACCAGT
    ATCATGGCCA ACGTGAAGAA AGCCTTTATT GGGGAAAACA GGGACCTTGT GGACCCTTAC
    GTCCTGGTGC AATTTGCAGG GCAAAAGGGC AAAACATCTG TTCAGAAGAG CAGCTACGAG
    CCCATCTGGA ACGAGCAAGT CATCTTCACG GAGATGTTCC CACCTTTGTG TAGGCGACTG
    AAAGTTCAGA TCCGTGATTC AGACAAGGTG AACGATGTTG CCATAGGAAC CCACTTCATT
    GATCTACGAA AGGTTTCCAA TGATGGAGAC AAAGGTTTCT TGCCCACTAT GGGTCCGGCT
    TGGGTGAATA TGTATGGGTC GACCCGTAAC TACACCCTGA TGGACGAACA CCAGGACCTG
    AATGAGGGGT TGGGGGAAGG GGTGTCCTTC AGAGCACGTC TGCTCATTAG TATCGCGGTG
    GAGATTCTAG ACACCACCTC TGCTGAGATC ATGAGCTCCA CTGAAGTACA CATGGAGCCG
    GTGTCCAACA TCTCAGAGAG TGCTACAGGA AAGATGGAAG AATTTTTCCT TTTCGGGTCA
    TTCTTGGAGG CCACAATGAT AGACAGAAAA ATAGGAGATA AACCCATCAG CTTTGAAGTC
    ACCATCGGTA ACTATGGTAA CCAGATTGAT GGTGTGAGCA AGCCTGCATT AGCGAAGAAG
    AAGAAGGAGG GTGGAGGAGA GAGTGAAGAA GAGGAGTCTG AGCTTATCCA CAACTCCAGC
    GAGGAAGAGG CAGAGGATGA TGGAGATCTG ACCTCTGTAC CGTCTACACC TCCTATGAAG
    CCTGTTATCA CCGACAGAAA TTACTTCCAC TTGCCTTACT TTGAAAAGAA ACCCTGCATT
    TACATCAAGA GCTGGTGGCA GGACCAGCGG AGACGTCTCT ACAACTCCAA CATAATGGAC
    AAGATAGCTG ATAAACTGGA GGAAGGTCTG AATGATGTTC AAGAGATCAT AAAAACAGAG
    AAGGCATATC CAGAACGAAG ACTAAGAGGT GTATTAGAAG AACTTAGCAC AAGCTGCAGC
    CGGTTTGTTA CACTTGCAAA CAAGGATCAG AATCTTTCTG GAAGAACCAA GCTGGACAGA
    GAGAGGCTGA AGTCCTGTAT GAGAGAAATG GAGAGTATGG GTCAGCAGGC GAAGACCATC
    CGCACACAAG TGAAAAGAAA CACAGTTCGA GATAAACTCA AGCTAGTGTT GAATTTCCTT
    CAGAGGCTTC GATTCTTAGC AGACGAGCCC CAGCACAGCA TCCCTGATGT GTTCATATGG
    ATGATTAGCA ACAACAAACG CATCGCATAC GCCCGCATAC CCTCCAAAGA CATCCTCTAC
    TCAATTGTCG ATGAAGAGAT GGGCAAAGAC TGTGGAAAAG TTAAAGCTGT TTTTCTCAGG
    CTGCCTGGAA AGAAAGGTTT TGGGCCTGCT GGCTGGACAG TTCAGGCTAA ATTAGAGATG
    TATTTGTGGC TGGGCCTGAA TAAACAGAGG AAAGACTTCC TGATTGGCCT CCCTAGTGGC
    TTTGAAGAAA ACAAGGCAGT AAAGGGAATT GGCATACAAG CTGTCCCTCC CATCAGCCTA
    GTTTATAACA TGAAGCAGGT GTTTCAGCTG AGGGCCCACA TGTATCAAGC TCGCAGTCTG
    TTTGCTGCGG ACACTAGTGG CCTGTCTGAT CCATTTGCAA GGGTTTTCTT CTCCACCCAC
    AGCCAGGTCA CGGAGGTCCT CAGTGAGACT CTCTGTCCAA CATGGGATCA GCTGCTTGTG
    TTTGATAATG TTGAACTTTA CGGTGAGGCC GGTGAACTCC GTGATGACCC TCCAATCATT
    GTCATTGAGT TGTATGATCA AGACACTGTG GGGAAAGCTG AGTTTATTGG CCGAACATTT
    GCAAAACCAC TTACTAAGAT GGTTGATGAA CACTACGGCC CACCGCGGTT TCCTCCCCAG
    CTCGAGTATT ATCAGATCTA CCGAGGAAAC TGTGCTGCGG GAGATCTATT GGCTGCTTTT
    GAGCTTCTAC AGATTGGTCC AGCAGGGCGG GCAGCACTTC CTCCAATCGA TGGACCGACC
    GATTCTGACC GTGGGCCCAT TCTGCCTGTT CCATTGGGAA TTCGACCAGT TCTCAGCAGA
    TATCGCATCG AGGTCCTGTT TTGGGGTCTG AGGGACCTTA AAAGGGTCAA CCTGGCTCAG
    GTGGACCGAC CTCGTGTGGA CATTGAGTGT GCAGGGAAAG GAGTTCAGTC AGCCCTAATT
    CAAAACTACA AGAAGAATCC AAACTTCAGC ACATTAGTAA AGTGGTTTGA AGTGGATCTG
    CCAGAAAATG AGCTGCTCCA TCCACCACTT AATATTCGGG TAGTGGATTG CAGGGCATTC
    GGACGCTACA TTTTGGTGGG GTCTCATGCT GTGACCACCC TTCGCAAGTT CATCTACAGC
    CCCCCAGACA AGACTGCTAA CAACTGGGCT CACACAGGTG ACATTGTGGT CAACATGAGC
    CCTGAACCTA ACATTAAGAA GATGGACACG GTTGTCAAGA TTGAAGCTAC CACCGATGCG
    GTTGTTAAAG TTGACTTGAA TGAGGATGAG AAGGAGAAAG AAAAGAAGAA GAAGAAGAAG
    AAGAAAGGAG AAGAAGTGGA GGAAGAGGAG CCGGATGAGA GCATGCTGGA CTGGTGGTCC
    AAATACTTTG CCTCAATAGA GACTATGATG GAGAATCTAA GAGCTCAGGA GGCCGCTCAG
    GCAGAGGCAG AGGAAAGAGA AGATTTGGAG ATTGCAGCAG AGAGTGCAGA GATCAAAGCT
    GATGACTTTC CTATGAAAGG CACCAAACCT AAAGAGAAGA GCAAAGACAA GAAGAGCACC
    AAGGACAAAA AGAAGAACAA CGATGGCACA GAAAAACGAC CTCCAAAACC AAAAGTTGAT
    GAACTCATGG TGTACAATAA AGAGCTGGAG AGTGAGTTCG GTAGCTTTGA GGACTGGCTT
    CACACCTTCA ACCTGTACCG AGGAAAGGCT GGAGATGATG ATGACCACAA TGTGGTTGAT
    GAGGACAGAA TTGTGGGCAG ATTCAAGGGC TCCCTCTGTA TGTATAAACT ACCACTGTCT
    GAGGAGATCA CCAGAGAGGC AGGATTTGAT CCAAACATGG GCATGTTCCA GAGTATTCCT
    CACAATGACC CTATCAACGT TCTTGTCAGG ATTTATATCA TTCGAGCCAC AGACCTGCAT
    CCTGCAGATA TAAATGGAAA GGCCGACCCT TATATAGTCA TTAAATTGGG AAAGTCAGAC
    ATTCGAGACA AGGAGAACTA CATATCCAAG CAGCTGAATC CTGTCTTTGG AAAGTCATTT
    GATATAGAGG CCACGTTCCC AATGGAGTCT ATGCTGACTG TGGCAGTGTA TGACTGGGAT
    TTAGTCGGAA CTGATGACCT GATTGGTGAG ACTAAGATTG ATTTGGAGAA CAGATACTAC
    AGCAAACACA GAGCCACATG TGGCATTGCT TCCAACTACT CTGTCCATGG TTATAATGTA
    TGGAGAGATC CTCAGAAACC AGCTCAGATC CTGGCTAAGT TGTGCAAAGA AGGTAAACTG
    GATGGACCTC ACTATGGACC TGGGGGAAGA GTCAAAGTTG CAAACCGGAT CTTTCTTGGG
    CCGACAGAAA TTGAGGATGA GAGTGGTCTG AAAAAACAAA CAGAAGAACA CTTGGCGCTC
    ACAGTCCTTA GACATTGGGA GGAAATCCCA CGTGTTGGTT GCAAACTCAT CCCTGAGCAT
    GTGGAGACGA GGCCTCTCCT CAACCCAGAC AAGCCAGGCA TAGAGCAGGG AAGGATTGAA
    ATGTGGGTGG ACATGTTTCC TATGGATGTC CCAGCACCAG GACCAGCCAT TGATATATCG
    CCACGCAAAC CAAAGAGATA TGAGCTCAGG GTGATAATAT GGAATACTGA CGAGGTAATT
    CTGGAAGACG ATGATTACTT CACAGGGGAA AAGTCCAGTG ACATTTTTGT GAGGGGCTGG
    CTTAAAGGAC AGCAGGAGGA CAAACAGGAC ACCGATGTAC ATTATCACTC TCTAACTGGA
    GAGGGAAACT TCAACTGGCG CTTTGTCTTC CCCTTTGACT ATCTCATGGC CGAGGAGAAG
    ATTGTTATCT CCAAGAAGGA ATCAATGTTT TCCTGGGATG AGACTGAGTA TAAGATCCCT
    GCTCGACTCA CCCTTCAAGT ATGGGATGCC GATCACTTTT CTGCAGATGA TTTTCTGGGT
    GCGATTGAGT TGGACTTGAA TAAGTTTCCG CGTGGGGCAA AAACAGCCAA GCAGTGCTCT
    CTAGACATGG TTCTCAAAGA GCATGAGCTG CCAACCATCT CCATCTTCAA ACAAAAGAGA
    GTCAAGGGCT GGTGGCCATT TGTGGCCCAA AATGAGAATG ATGAATTTGA GCTAACGGGA
    AAAGTAGAGG CAGAGCTCCA TCTGTTGACA GCAGAGGAGG CAGAGAAGAG TCCAGTCGGC
    CTGGGGAGGA ATGATCCGGA GCCACTTGAG AAACCAAACC GGCCCGACAC CACCTTCCTG
    TGGTTTCTGA GCCCACTGAA GGCCATCCGC TACCTGATAT GCAACCGCTA CAAGTGGCTC
    ATCATCAAGA TTGTGTTGGC TCTGCTGCTG CTGATCATGG TGGGCCTGTT TCTCTACAGC
    ATGCCAGGCT ATCTGGTCAA GAAGCTGCTC GGGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.