NEK1 gene cDNA ORF clone, Danio rerio(Zebrafish)

mRNA	Protein	Name
XM_009294678.3	XP_009292953.1	serine/threonine-protein kinase Nek1 isoform X2
XM_009294680.2	XP_009292955.1	serine/threonine-protein kinase Nek1 isoform X4
XM_009294682.2	XP_009292957.1	serine/threonine-protein kinase Nek1 isoform X6
XM_009294681.3	XP_009292956.1	serine/threonine-protein kinase Nek1 isoform X5
XM_009294678.2	XP_009292953.1	serine/threonine-protein kinase Nek1 isoform X2
XM_009294682.3	XP_009292957.1	serine/threonine-protein kinase Nek1 isoform X6
XM_009294677.3	XP_009292952.1	serine/threonine-protein kinase Nek1 isoform X1
XM_009294679.3	XP_009292954.1	serine/threonine-protein kinase Nek1 isoform X3
XM_009294680.3	XP_009292955.1	serine/threonine-protein kinase Nek1 isoform X4
XM_009294677.2	XP_009292952.1	serine/threonine-protein kinase Nek1 isoform X1
XM_009294679.2	XP_009292954.1	serine/threonine-protein kinase Nek1 isoform X3
XM_009294681.2	XP_009292956.1	serine/threonine-protein kinase Nek1 isoform X5

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa42282	XM_009294677.3 Latest version!	Danio rerio NIMA-related kinase 1 (nek1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa42283	XM_009294678.3 Latest version!	Danio rerio NIMA-related kinase 1 (nek1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa42284	XM_009294679.3 Latest version!	Danio rerio NIMA-related kinase 1 (nek1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa42285	XM_009294680.3 Latest version!	Danio rerio NIMA-related kinase 1 (nek1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa42286	XM_009294681.3 Latest version!	Danio rerio NIMA-related kinase 1 (nek1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa42287	XM_009294682.3 Latest version!	Danio rerio NIMA-related kinase 1 (nek1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
View Old Accession Versions >>
ODa42283	XM_009294678.2	Danio rerio NIMA-related kinase 1 (nek1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa42287	XM_009294682.2	Danio rerio NIMA-related kinase 1 (nek1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa42285	XM_009294680.2	Danio rerio NIMA-related kinase 1 (nek1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa42282	XM_009294677.2	Danio rerio NIMA-related kinase 1 (nek1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa42284	XM_009294679.2	Danio rerio NIMA-related kinase 1 (nek1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa42286	XM_009294681.2	Danio rerio NIMA-related kinase 1 (nek1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
Hide Old Accession Versions >>

NEK1 ( XM_009294677.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009292952.1 Danio rerio NIMA-related kinase 1 (nek1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa42282
Clone ID Related Accession (Same CDS sequence)	XM_009294677.3 , XM_009294677.2
Accession Version	XM_009294677.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4242bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase Nek1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009294677.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAAAT ATGAGAGGTT AAAGAAGATT GGGGAGGGAT CCTTTGGGAA AGCCATCTTG 
GTCAAGTCAA GGACAGATGG CCGACAATAT GTTATCAAGG AGATTGGTAT CTCCAGGATG 
TCGAACAAGG AACGACAGGA ATCACGTAAA GAGGTGGCAG TTCTGGCTAA CATGAGCCAT 
CCAAACATTG TCCAATACAA GGAATCCTTT GAAGAGAGTG GTTGTTTGTA CATTGTAATG 
GACTACTGTG AGGGAGGAGA CCTGTTTAAG AAGATCAATA ATCAAAGAGG ATCTCTTTTC 
CCAGAGGAAC AGATTCTTGA CTGGTTTGTG CAAATCTGTC TGGCTCTTAA ACATGTACAC 
GACCGCAAAA TCCTACATAG GGACATCAAA TCACAGAATA TTTTTCTGAC CAAAGATGGT 
ACTGTGCAGC TTGGAGACTT TGGAATCGCT AGAGTTTTGA ACAGCACCGT GGAGTTGGCA 
AGGACATGTA TCGGCACTCC TTATTACCTG TCGCCAGAGA TATGTGAAAA TAAACCATAC 
AACAACAAGA GTGATATCTG GGCCCTGGGA TGTGTCCTGT ATGAAATGTG CACGCTTAAG 
CATGCATTTG AAGCAGGAAA CATGAAGAAC CTGGTGCTGA AAATCATCCG TGGTTCATAC 
CCGCCTGTTT CCATCCATTA CTCCCCAGAT TTGCGCTCTC TCCTGGCCCA GCTCTTCAAG 
CGTAACCCCC GGGAGAGACC CTCCGTCTCG ACCATTTTGG ACAAGCCTTT TCTTGCTCGC 
AGAATTCACA AGTTTCTCAG CCCTCAGCTT ATTGCTCAGG AATTCAGCCA TTCAATTCAC 
CTTCAGCCCA AAATGAGTGT GGCCCATGCA GCACCAGCCA AACGTCCAGC CCCAGGCCCT 
ATCCCAATCA CCTCAGCTCA GAAAATCACC AAACCAGCAG CCAAATACGG AGTGCCTTTA 
ACAGTGCGGA GACCATCTGA AGTCGCCAGG AAGGTCCCAG ATGCAGCAAA GAAACCAGTT 
AAACTTAAGC AGGCTCCCCC TCCTGCAGCG GTTCAGAGGA GAGTGAGTCG AGTGGAGGAG 
GAGAGGAGAA AGCATGAGGA AGGAGTGAGG AAGAAGCGGA TGGAGATGAT GGAAAGAGAG 
AGAAAACAAA GAGAACAGAT GTTGTTACTG AAGGCTGCTC AGATGAAGAG ATTTGAGAGA 
GAAAAGCTGA ACCGAATCAA CCGAGCTCGA GAGCAGGGCT GGAGACACGT GCTGAGTTCT 
AGTGGAGGAA GCAGTCCTGA GCGGAAGTTT TATGGAGGTG GCGGCGGCAA TGGAGCTCCC 
CATTCCCCAG GAATATCTCC CTTTCCTGTG CCTGGACCCT CCCCTGTTCC TTCTTCAGCT 
TTAGCTCCAA GCAGAGCGCC ATATGACCGC ATCCACCCTT CAGCTGAAAA ATTAACCAAA 
CCTACACCTA AAGATGGTGC TGGTGAAGGA GCCGTGCATG CAGGGGACAA TGGCAGGACT 
TCTTCAGACT CAGGGACTCT AAATGGTCAT GCCTGCATTC CTGTTAAGAA TGGCGTCAGG 
AAAGAACAAC GTAAAACAAG TCTCCAAAAT GAGCAGATTT CAAAGATCGA CTATGCCAAT 
AGGTTTCGAG GTCAGCTGGC AGTAGAGAAA TCCAAGCAGG TGGAGGAGTT TCTACAGCGG 
AAAAGAGAAG CCATGCTGAA TAAAGTTCGT GCAGAAGGAC AGCTGGGGGC CCGGCAAAAC 
CTGGCCGCCA TCTTTGCAAG CTGGACAAAC TCCTCCCGAT GCAGGAGACC CCGAGTGAAC 
AAAGAGGAGG AGGAGTATTT GACCAGATTA CGACAGATCA GACTACAGAA CTTTAATGAA 
CGTCAGCAGA TTAAAGCTCG GCTCAGAGGG GAGAAGTATG ACAGTGATGG CTCAGACAGT 
CAGGAGTCAT GTGAAGAGGC AGTGCTCAGG ATGAAGAAGA TTGAGGCTTT GAAGGCCCAG 
TCAAAGGCTC GTGCTGCTGT ACTGAAAGAG CAGCTGGAAA AGAAGAGAAG AGAAGCGTAT 
GAAAGAGAGA AGAAAGCATG GGAGGATCAT CAGCTTGTCG CTCGAGGAGT AAAAGTTGCA 
GTTGGGCCTG TGGGTGGGAC TCCCTCTCCT GCAGCCCCTC CAACTCCACC ATCTGACCCC 
ACCCCTCAGG AAGCTCCTTC TAAAGCCACA CCCTCCTCTA GACCCAGCAC TCCAGCCATC 
TCTATGACTG CTGCACTGAA GGATGTGGGC GCGATTTCAT CAGGGCAGAG TTCTCCTATG 
AAAGAGGACT CCCTTAAAGT TGAATCATCA GATGTACAGA GTGACAAGAA GGAGATTCTT 
CGCAGACTTA ATCAGAAGGT TCAGACCACT GAGGAAGTTT CTGTAAAAGA GCCCACACCC 
TCCAGCAATC AACCATGTCC TGATCCTCAG CCAGTCCAAC CCTCCATCAC AGAGGAAAGG 
CCTTCCACAG CTGCAGATAG GAAGAAGTGG GAAGCCGTAG CTCCACCCAT ACTGTCTGTT 
GCTGAACAGA CTCTCGAGGA AACCTGTGCT ACTCTGGCAG TGTCTCAGGT GGCATCACCA 
GAAACGAATA CTCCCTCGTG TGGAGACAGG AAGAAATGGC AGACAGATGG TGTTCCTCTT 
CTGTCTGTGG CTCAGCAGAC ACTGGAAGAA ACCAGCTTTA GAACAGCTGG TCAATTAACA 
GAAAAAACAG TAGGTGAAGT CATTTGCATG GATGCCTGGC AGGGGGAGGG GCTAAGGAAG 
GCTTGGGGGC AGAGTCCAGA TTCAGAGGTT TTGAAGGTGT TGGAAGAGGC AGAACTTCAG 
TCATTTACTC AGAGACTAGA TCCTGCCAGC ACGTGTGACC AAACACTGAC TGAAAGCCTT 
AATCCCATTG AGGAATCGGA TGCCACTAAT GAAGCCAAGC AAGTGCCTCC TCCTGAACCG 
TGCCAGCCTG CCACTGACCA TGACAAACAA GAAGTGACAT GTCAAGAGAC AGTGCCAATC 
ATAGAAGAGG TTAAAAGTCA GGAACTTATC CAAACAACAG CAGAGGGGAC AGAAAAATCT 
CATTCTGCAG AAAGTGACAT AGAGGTGGTG GATGATATGG AGTCAGTTGT TCTGGATGAT 
GTACCACAGA AAACCGCAGA GCCGCCCCCT GCAGAAGTAA TTCAGGCGTG GACTAAAGAC 
ACTCCAGAGT CTCTGCCAGC AGAGGGCAGA GTAATACAAG CAGAATGCAC AGAGGTACCT 
CAGAGAGAAG AGAAACATCC TGAACCTCCA GCAGATGAGT CTTTGTTTGT GAAGTTAAGC 
AGTAGTCCAG CTCACAGACG CACAGTAGCG TTGGCCATGC TGTCTGCTCA ATCCTCATTG 
GAAGACTCTT CCTCTGCTGC CTCACGTTCT CGATCAGTGT CTCCACTGCG CTCCCACCGC 
CATGAAGATG CACTGCTCAT CGGCCTCTCC ACTGGCCTCT TCGATGCAAA TAACCCAAAG 
ATGCTAAGGA CGTGCTCCTT ACCAGACCTC AGTAAGGTGT TTAGTGATGC TACAGGACCT 
GAAGGTGCAG CCGCTCCTGA CAGCAACCTA GAAATTGAAG ATCTGGAAGA TAAAGCAGAT 
GAGCAGTCTG AACCTGAGGA TGTGTATGAG GATGACGATT TAAGAGAGCT CAGAGCATCT 
ATGGAGAGGC TTCTTCAGGA GCAGCACAGT GAAGATGATG ATGATGAAGA AGAGGATGAG 
GGTGGTGGAT CTAATGGCAG CCCGGCCGAT GAAGAGCCAG CTGGTCTTAA TGGGTTAGCT 
AGTGCTGGTG ATGACAAACG CAGCCCAGAG GAAGAGACTT TTAATGGAAT GGCTGAAGAT 
GATGAGGATG TTAGCCATGG TGAAGAAGAA CCAGGAGGCC CAGTCACAAA TGGAATGGAA 
TTTGAAGATG ATGAAGAGCA GAACAGCAGT GAAGCACAGC TCAATGAGGA GTGGCAGTCA 
GATGGCAGCG AGGAGGAGGA CGACGAAGAG CTGGAACAGC AGGACAGCAT CTTCAGTCGT 
CTGGAGGAGC TGCGGCTTCA TCTAGAGCAG GCCATGGGCT TTGAGAACTT CATCCAGGCC 
TACAACAAGA TCAAGGCTAT TCATGAGGAT GAGGATGAAA ACATTGCAAT GGGCTCCAGC 
ATGGTGCAAA ACATTTTGGG AACTGAGCAC CAGCACTTGT ATCCTAAAAT CCTCCATTTG 
GTGATGGCAG ATGGAGCTTA CCAGGAAGAT AATGATGAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009292952.1
CDS	127..4368
Translation	MDKYERLKKIGEGSFGKAILVKSRTDGRQYVIKEIGISRMSNKERQESRKEVAVLANMSHPNIVQYKESFEESGCLYIVMDYCEGGDLFKKINNQRGSLFPEEQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIIRGSYPPVSIHYSPDLRSLLAQLFKRNPRERPSVSTILDKPFLARRIHKFLSPQLIAQEFSHSIHLQPKMSVAHAAPAKRPAPGPIPITSAQKITKPAAKYGVPLTVRRPSEVARKVPDAAKKPVKLKQAPPPAAVQRRVSRVEEERRKHEEGVRKKRMEMMERERKQREQMLLLKAAQMKRFEREKLNRINRAREQGWRHVLSSSGGSSPERKFYGGGGGNGAPHSPGISPFPVPGPSPVPSSALAPSRAPYDRIHPSAEKLTKPTPKDGAGEGAVHAGDNGRTSSDSGTLNGHACIPVKNGVRKEQRKTSLQNEQISKIDYANRFRGQLAVEKSKQVEEFLQRKREAMLNKVRAEGQLGARQNLAAIFASWTNSSRCRRPRVNKEEEEYLTRLRQIRLQNFNERQQIKARLRGEKYDSDGSDSQESCEEAVLRMKKIEALKAQSKARAAVLKEQLEKKRREAYEREKKAWEDHQLVARGVKVAVGPVGGTPSPAAPPTPPSDPTPQEAPSKATPSSRPSTPAISMTAALKDVGAISSGQSSPMKEDSLKVESSDVQSDKKEILRRLNQKVQTTEEVSVKEPTPSSNQPCPDPQPVQPSITEERPSTAADRKKWEAVAPPILSVAEQTLEETCATLAVSQVASPETNTPSCGDRKKWQTDGVPLLSVAQQTLEETSFRTAGQLTEKTVGEVICMDAWQGEGLRKAWGQSPDSEVLKVLEEAELQSFTQRLDPASTCDQTLTESLNPIEESDATNEAKQVPPPEPCQPATDHDKQEVTCQETVPIIEEVKSQELIQTTAEGTEKSHSAESDIEVVDDMESVVLDDVPQKTAEPPPAEVIQAWTKDTPESLPAEGRVIQAECTEVPQREEKHPEPPADESLFVKLSSSPAHRRTVALAMLSAQSSLEDSSSAASRSRSVSPLRSHRHEDALLIGLSTGLFDANNPKMLRTCSLPDLSKVFSDATGPEGAAAPDSNLEIEDLEDKADEQSEPEDVYEDDDLRELRASMERLLQEQHSEDDDDEEEDEGGGSNGSPADEEPAGLNGLASAGDDKRSPEEETFNGMAEDDEDVSHGEEEPGGPVTNGMEFEDDEEQNSSEAQLNEEWQSDGSEEEDDEELEQQDSIFSRLEELRLHLEQAMGFENFIQAYNKIKAIHEDEDENIAMGSSMVQNILGTEHQHLYPKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_009294677.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio NIMA-related kinase 1 (nek1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009294677.3

ORF Insert Sequence:

ATGGATAAAT ATGAGAGGTT AAAGAAGATT GGGGAGGGAT CCTTTGGGAA AGCCATCTTG 
GTCAAGTCAA GGACAGATGG CCGACAATAT GTTATCAAGG AGATTGGTAT CTCCAGGATG 
TCGAACAAGG AACGACAGGA ATCACGTAAA GAGGTGGCAG TTCTGGCTAA CATGAGCCAT 
CCAAACATTG TCCAATACAA GGAATCCTTT GAAGAGAGTG GTTGTTTGTA CATTGTAATG 
GACTACTGTG AGGGAGGAGA CCTGTTTAAG AAGATCAATA ATCAAAGAGG ATCTCTTTTC 
CCAGAGGAAC AGATTCTTGA CTGGTTTGTG CAAATCTGTC TGGCTCTTAA ACATGTACAC 
GACCGCAAAA TCCTACATAG GGACATCAAA TCACAGAATA TTTTTCTGAC CAAAGATGGT 
ACTGTGCAGC TTGGAGACTT TGGAATCGCT AGAGTTTTGA ACAGCACCGT GGAGTTGGCA 
AGGACATGTA TCGGCACTCC TTATTACCTG TCGCCAGAGA TATGTGAAAA TAAACCATAC 
AACAACAAGA GTGATATCTG GGCCCTGGGA TGTGTCCTGT ATGAAATGTG CACGCTTAAG 
CATGCATTTG AAGCAGGAAA CATGAAGAAC CTGGTGCTGA AAATCATCCG TGGTTCATAC 
CCGCCTGTTT CCATCCATTA CTCCCCAGAT TTGCGCTCTC TCCTGGCCCA GCTCTTCAAG 
CGTAACCCCC GGGAGAGACC CTCCGTCTCG ACCATTTTGG ACAAGCCTTT TCTTGCTCGC 
AGAATTCACA AGTTTCTCAG CCCTCAGCTT ATTGCTCAGG AATTCAGCCA TTCAATTCAC 
CTTCAGCCCA AAATGAGTGT GGCCCATGCA GCACCAGCCA AACGTCCAGC CCCAGGCCCT 
ATCCCAATCA CCTCAGCTCA GAAAATCACC AAACCAGCAG CCAAATACGG AGTGCCTTTA 
ACAGTGCGGA GACCATCTGA AGTCGCCAGG AAGGTCCCAG ATGCAGCAAA GAAACCAGTT 
AAACTTAAGC AGGCTCCCCC TCCTGCAGCG GTTCAGAGGA GAGTGAGTCG AGTGGAGGAG 
GAGAGGAGAA AGCATGAGGA AGGAGTGAGG AAGAAGCGGA TGGAGATGAT GGAAAGAGAG 
AGAAAACAAA GAGAACAGAT GTTGTTACTG AAGGCTGCTC AGATGAAGAG ATTTGAGAGA 
GAAAAGCTGA ACCGAATCAA CCGAGCTCGA GAGCAGGGCT GGAGACACGT GCTGAGTTCT 
AGTGGAGGAA GCAGTCCTGA GCGGAAGTTT TATGGAGGTG GCGGCGGCAA TGGAGCTCCC 
CATTCCCCAG GAATATCTCC CTTTCCTGTG CCTGGACCCT CCCCTGTTCC TTCTTCAGCT 
TTAGCTCCAA GCAGAGCGCC ATATGACCGC ATCCACCCTT CAGCTGAAAA ATTAACCAAA 
CCTACACCTA AAGATGGTGC TGGTGAAGGA GCCGTGCATG CAGGGGACAA TGGCAGGACT 
TCTTCAGACT CAGGGACTCT AAATGGTCAT GCCTGCATTC CTGTTAAGAA TGGCGTCAGG 
AAAGAACAAC GTAAAACAAG TCTCCAAAAT GAGCAGATTT CAAAGATCGA CTATGCCAAT 
AGGTTTCGAG GTCAGCTGGC AGTAGAGAAA TCCAAGCAGG TGGAGGAGTT TCTACAGCGG 
AAAAGAGAAG CCATGCTGAA TAAAGTTCGT GCAGAAGGAC AGCTGGGGGC CCGGCAAAAC 
CTGGCCGCCA TCTTTGCAAG CTGGACAAAC TCCTCCCGAT GCAGGAGACC CCGAGTGAAC 
AAAGAGGAGG AGGAGTATTT GACCAGATTA CGACAGATCA GACTACAGAA CTTTAATGAA 
CGTCAGCAGA TTAAAGCTCG GCTCAGAGGG GAGAAGTATG ACAGTGATGG CTCAGACAGT 
CAGGAGTCAT GTGAAGAGGC AGTGCTCAGG ATGAAGAAGA TTGAGGCTTT GAAGGCCCAG 
TCAAAGGCTC GTGCTGCTGT ACTGAAAGAG CAGCTGGAAA AGAAGAGAAG AGAAGCGTAT 
GAAAGAGAGA AGAAAGCATG GGAGGATCAT CAGCTTGTCG CTCGAGGAGT AAAAGTTGCA 
GTTGGGCCTG TGGGTGGGAC TCCCTCTCCT GCAGCCCCTC CAACTCCACC ATCTGACCCC 
ACCCCTCAGG AAGCTCCTTC TAAAGCCACA CCCTCCTCTA GACCCAGCAC TCCAGCCATC 
TCTATGACTG CTGCACTGAA GGATGTGGGC GCGATTTCAT CAGGGCAGAG TTCTCCTATG 
AAAGAGGACT CCCTTAAAGT TGAATCATCA GATGTACAGA GTGACAAGAA GGAGATTCTT 
CGCAGACTTA ATCAGAAGGT TCAGACCACT GAGGAAGTTT CTGTAAAAGA GCCCACACCC 
TCCAGCAATC AACCATGTCC TGATCCTCAG CCAGTCCAAC CCTCCATCAC AGAGGAAAGG 
CCTTCCACAG CTGCAGATAG GAAGAAGTGG GAAGCCGTAG CTCCACCCAT ACTGTCTGTT 
GCTGAACAGA CTCTCGAGGA AACCTGTGCT ACTCTGGCAG TGTCTCAGGT GGCATCACCA 
GAAACGAATA CTCCCTCGTG TGGAGACAGG AAGAAATGGC AGACAGATGG TGTTCCTCTT 
CTGTCTGTGG CTCAGCAGAC ACTGGAAGAA ACCAGCTTTA GAACAGCTGG TCAATTAACA 
GAAAAAACAG TAGGTGAAGT CATTTGCATG GATGCCTGGC AGGGGGAGGG GCTAAGGAAG 
GCTTGGGGGC AGAGTCCAGA TTCAGAGGTT TTGAAGGTGT TGGAAGAGGC AGAACTTCAG 
TCATTTACTC AGAGACTAGA TCCTGCCAGC ACGTGTGACC AAACACTGAC TGAAAGCCTT 
AATCCCATTG AGGAATCGGA TGCCACTAAT GAAGCCAAGC AAGTGCCTCC TCCTGAACCG 
TGCCAGCCTG CCACTGACCA TGACAAACAA GAAGTGACAT GTCAAGAGAC AGTGCCAATC 
ATAGAAGAGG TTAAAAGTCA GGAACTTATC CAAACAACAG CAGAGGGGAC AGAAAAATCT 
CATTCTGCAG AAAGTGACAT AGAGGTGGTG GATGATATGG AGTCAGTTGT TCTGGATGAT 
GTACCACAGA AAACCGCAGA GCCGCCCCCT GCAGAAGTAA TTCAGGCGTG GACTAAAGAC 
ACTCCAGAGT CTCTGCCAGC AGAGGGCAGA GTAATACAAG CAGAATGCAC AGAGGTACCT 
CAGAGAGAAG AGAAACATCC TGAACCTCCA GCAGATGAGT CTTTGTTTGT GAAGTTAAGC 
AGTAGTCCAG CTCACAGACG CACAGTAGCG TTGGCCATGC TGTCTGCTCA ATCCTCATTG 
GAAGACTCTT CCTCTGCTGC CTCACGTTCT CGATCAGTGT CTCCACTGCG CTCCCACCGC 
CATGAAGATG CACTGCTCAT CGGCCTCTCC ACTGGCCTCT TCGATGCAAA TAACCCAAAG 
ATGCTAAGGA CGTGCTCCTT ACCAGACCTC AGTAAGGTGT TTAGTGATGC TACAGGACCT 
GAAGGTGCAG CCGCTCCTGA CAGCAACCTA GAAATTGAAG ATCTGGAAGA TAAAGCAGAT 
GAGCAGTCTG AACCTGAGGA TGTGTATGAG GATGACGATT TAAGAGAGCT CAGAGCATCT 
ATGGAGAGGC TTCTTCAGGA GCAGCACAGT GAAGATGATG ATGATGAAGA AGAGGATGAG 
GGTGGTGGAT CTAATGGCAG CCCGGCCGAT GAAGAGCCAG CTGGTCTTAA TGGGTTAGCT 
AGTGCTGGTG ATGACAAACG CAGCCCAGAG GAAGAGACTT TTAATGGAAT GGCTGAAGAT 
GATGAGGATG TTAGCCATGG TGAAGAAGAA CCAGGAGGCC CAGTCACAAA TGGAATGGAA 
TTTGAAGATG ATGAAGAGCA GAACAGCAGT GAAGCACAGC TCAATGAGGA GTGGCAGTCA 
GATGGCAGCG AGGAGGAGGA CGACGAAGAG CTGGAACAGC AGGACAGCAT CTTCAGTCGT 
CTGGAGGAGC TGCGGCTTCA TCTAGAGCAG GCCATGGGCT TTGAGAACTT CATCCAGGCC 
TACAACAAGA TCAAGGCTAT TCATGAGGAT GAGGATGAAA ACATTGCAAT GGGCTCCAGC 
ATGGTGCAAA ACATTTTGGG AACTGAGCAC CAGCACTTGT ATCCTAAAAT CCTCCATTTG 
GTGATGGCAG ATGGAGCTTA CCAGGAAGAT AATGATGAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_009294677.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009292952.1 Danio rerio NIMA-related kinase 1 (nek1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa42282
Clone ID Related Accession (Same CDS sequence)	XM_009294677.3 , XM_009294677.2
Accession Version	XM_009294677.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4242bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	serine/threonine-protein kinase Nek1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009294677.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAAAT ATGAGAGGTT AAAGAAGATT GGGGAGGGAT CCTTTGGGAA AGCCATCTTG 
GTCAAGTCAA GGACAGATGG CCGACAATAT GTTATCAAGG AGATTGGTAT CTCCAGGATG 
TCGAACAAGG AACGACAGGA ATCACGTAAA GAGGTGGCAG TTCTGGCTAA CATGAGCCAT 
CCAAACATTG TCCAATACAA GGAATCCTTT GAAGAGAGTG GTTGTTTGTA CATTGTAATG 
GACTACTGTG AGGGAGGAGA CCTGTTTAAG AAGATCAATA ATCAAAGAGG ATCTCTTTTC 
CCAGAGGAAC AGATTCTTGA CTGGTTTGTG CAAATCTGTC TGGCTCTTAA ACATGTACAC 
GACCGCAAAA TCCTACATAG GGACATCAAA TCACAGAATA TTTTTCTGAC CAAAGATGGT 
ACTGTGCAGC TTGGAGACTT TGGAATCGCT AGAGTTTTGA ACAGCACCGT GGAGTTGGCA 
AGGACATGTA TCGGCACTCC TTATTACCTG TCGCCAGAGA TATGTGAAAA TAAACCATAC 
AACAACAAGA GTGATATCTG GGCCCTGGGA TGTGTCCTGT ATGAAATGTG CACGCTTAAG 
CATGCATTTG AAGCAGGAAA CATGAAGAAC CTGGTGCTGA AAATCATCCG TGGTTCATAC 
CCGCCTGTTT CCATCCATTA CTCCCCAGAT TTGCGCTCTC TCCTGGCCCA GCTCTTCAAG 
CGTAACCCCC GGGAGAGACC CTCCGTCTCG ACCATTTTGG ACAAGCCTTT TCTTGCTCGC 
AGAATTCACA AGTTTCTCAG CCCTCAGCTT ATTGCTCAGG AATTCAGCCA TTCAATTCAC 
CTTCAGCCCA AAATGAGTGT GGCCCATGCA GCACCAGCCA AACGTCCAGC CCCAGGCCCT 
ATCCCAATCA CCTCAGCTCA GAAAATCACC AAACCAGCAG CCAAATACGG AGTGCCTTTA 
ACAGTGCGGA GACCATCTGA AGTCGCCAGG AAGGTCCCAG ATGCAGCAAA GAAACCAGTT 
AAACTTAAGC AGGCTCCCCC TCCTGCAGCG GTTCAGAGGA GAGTGAGTCG AGTGGAGGAG 
GAGAGGAGAA AGCATGAGGA AGGAGTGAGG AAGAAGCGGA TGGAGATGAT GGAAAGAGAG 
AGAAAACAAA GAGAACAGAT GTTGTTACTG AAGGCTGCTC AGATGAAGAG ATTTGAGAGA 
GAAAAGCTGA ACCGAATCAA CCGAGCTCGA GAGCAGGGCT GGAGACACGT GCTGAGTTCT 
AGTGGAGGAA GCAGTCCTGA GCGGAAGTTT TATGGAGGTG GCGGCGGCAA TGGAGCTCCC 
CATTCCCCAG GAATATCTCC CTTTCCTGTG CCTGGACCCT CCCCTGTTCC TTCTTCAGCT 
TTAGCTCCAA GCAGAGCGCC ATATGACCGC ATCCACCCTT CAGCTGAAAA ATTAACCAAA 
CCTACACCTA AAGATGGTGC TGGTGAAGGA GCCGTGCATG CAGGGGACAA TGGCAGGACT 
TCTTCAGACT CAGGGACTCT AAATGGTCAT GCCTGCATTC CTGTTAAGAA TGGCGTCAGG 
AAAGAACAAC GTAAAACAAG TCTCCAAAAT GAGCAGATTT CAAAGATCGA CTATGCCAAT 
AGGTTTCGAG GTCAGCTGGC AGTAGAGAAA TCCAAGCAGG TGGAGGAGTT TCTACAGCGG 
AAAAGAGAAG CCATGCTGAA TAAAGTTCGT GCAGAAGGAC AGCTGGGGGC CCGGCAAAAC 
CTGGCCGCCA TCTTTGCAAG CTGGACAAAC TCCTCCCGAT GCAGGAGACC CCGAGTGAAC 
AAAGAGGAGG AGGAGTATTT GACCAGATTA CGACAGATCA GACTACAGAA CTTTAATGAA 
CGTCAGCAGA TTAAAGCTCG GCTCAGAGGG GAGAAGTATG ACAGTGATGG CTCAGACAGT 
CAGGAGTCAT GTGAAGAGGC AGTGCTCAGG ATGAAGAAGA TTGAGGCTTT GAAGGCCCAG 
TCAAAGGCTC GTGCTGCTGT ACTGAAAGAG CAGCTGGAAA AGAAGAGAAG AGAAGCGTAT 
GAAAGAGAGA AGAAAGCATG GGAGGATCAT CAGCTTGTCG CTCGAGGAGT AAAAGTTGCA 
GTTGGGCCTG TGGGTGGGAC TCCCTCTCCT GCAGCCCCTC CAACTCCACC ATCTGACCCC 
ACCCCTCAGG AAGCTCCTTC TAAAGCCACA CCCTCCTCTA GACCCAGCAC TCCAGCCATC 
TCTATGACTG CTGCACTGAA GGATGTGGGC GCGATTTCAT CAGGGCAGAG TTCTCCTATG 
AAAGAGGACT CCCTTAAAGT TGAATCATCA GATGTACAGA GTGACAAGAA GGAGATTCTT 
CGCAGACTTA ATCAGAAGGT TCAGACCACT GAGGAAGTTT CTGTAAAAGA GCCCACACCC 
TCCAGCAATC AACCATGTCC TGATCCTCAG CCAGTCCAAC CCTCCATCAC AGAGGAAAGG 
CCTTCCACAG CTGCAGATAG GAAGAAGTGG GAAGCCGTAG CTCCACCCAT ACTGTCTGTT 
GCTGAACAGA CTCTCGAGGA AACCTGTGCT ACTCTGGCAG TGTCTCAGGT GGCATCACCA 
GAAACGAATA CTCCCTCGTG TGGAGACAGG AAGAAATGGC AGACAGATGG TGTTCCTCTT 
CTGTCTGTGG CTCAGCAGAC ACTGGAAGAA ACCAGCTTTA GAACAGCTGG TCAATTAACA 
GAAAAAACAG TAGGTGAAGT CATTTGCATG GATGCCTGGC AGGGGGAGGG GCTAAGGAAG 
GCTTGGGGGC AGAGTCCAGA TTCAGAGGTT TTGAAGGTGT TGGAAGAGGC AGAACTTCAG 
TCATTTACTC AGAGACTAGA TCCTGCCAGC ACGTGTGACC AAACACTGAC TGAAAGCCTT 
AATCCCATTG AGGAATCGGA TGCCACTAAT GAAGCCAAGC AAGTGCCTCC TCCTGAACCG 
TGCCAGCCTG CCACTGACCA TGACAAACAA GAAGTGACAT GTCAAGAGAC AGTGCCAATC 
ATAGAAGAGG TTAAAAGTCA GGAACTTATC CAAACAACAG CAGAGGGGAC AGAAAAATCT 
CATTCTGCAG AAAGTGACAT AGAGGTGGTG GATGATATGG AGTCAGTTGT TCTGGATGAT 
GTACCACAGA AAACCGCAGA GCCGCCCCCT GCAGAAGTAA TTCAGGCGTG GACTAAAGAC 
ACTCCAGAGT CTCTGCCAGC AGAGGGCAGA GTAATACAAG CAGAATGCAC AGAGGTACCT 
CAGAGAGAAG AGAAACATCC TGAACCTCCA GCAGATGAGT CTTTGTTTGT GAAGTTAAGC 
AGTAGTCCAG CTCACAGACG CACAGTAGCG TTGGCCATGC TGTCTGCTCA ATCCTCATTG 
GAAGACTCTT CCTCTGCTGC CTCACGTTCT CGATCAGTGT CTCCACTGCG CTCCCACCGC 
CATGAAGATG CACTGCTCAT CGGCCTCTCC ACTGGCCTCT TCGATGCAAA TAACCCAAAG 
ATGCTAAGGA CGTGCTCCTT ACCAGACCTC AGTAAGGTGT TTAGTGATGC TACAGGACCT 
GAAGGTGCAG CCGCTCCTGA CAGCAACCTA GAAATTGAAG ATCTGGAAGA TAAAGCAGAT 
GAGCAGTCTG AACCTGAGGA TGTGTATGAG GATGACGATT TAAGAGAGCT CAGAGCATCT 
ATGGAGAGGC TTCTTCAGGA GCAGCACAGT GAAGATGATG ATGATGAAGA AGAGGATGAG 
GGTGGTGGAT CTAATGGCAG CCCGGCCGAT GAAGAGCCAG CTGGTCTTAA TGGGTTAGCT 
AGTGCTGGTG ATGACAAACG CAGCCCAGAG GAAGAGACTT TTAATGGAAT GGCTGAAGAT 
GATGAGGATG TTAGCCATGG TGAAGAAGAA CCAGGAGGCC CAGTCACAAA TGGAATGGAA 
TTTGAAGATG ATGAAGAGCA GAACAGCAGT GAAGCACAGC TCAATGAGGA GTGGCAGTCA 
GATGGCAGCG AGGAGGAGGA CGACGAAGAG CTGGAACAGC AGGACAGCAT CTTCAGTCGT 
CTGGAGGAGC TGCGGCTTCA TCTAGAGCAG GCCATGGGCT TTGAGAACTT CATCCAGGCC 
TACAACAAGA TCAAGGCTAT TCATGAGGAT GAGGATGAAA ACATTGCAAT GGGCTCCAGC 
ATGGTGCAAA ACATTTTGGG AACTGAGCAC CAGCACTTGT ATCCTAAAAT CCTCCATTTG 
GTGATGGCAG ATGGAGCTTA CCAGGAAGAT AATGATGAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009292952.1
CDS	118..4359
Translation	MDKYERLKKIGEGSFGKAILVKSRTDGRQYVIKEIGISRMSNKERQESRKEVAVLANMSHPNIVQYKESFEESGCLYIVMDYCEGGDLFKKINNQRGSLFPEEQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIIRGSYPPVSIHYSPDLRSLLAQLFKRNPRERPSVSTILDKPFLARRIHKFLSPQLIAQEFSHSIHLQPKMSVAHAAPAKRPAPGPIPITSAQKITKPAAKYGVPLTVRRPSEVARKVPDAAKKPVKLKQAPPPAAVQRRVSRVEEERRKHEEGVRKKRMEMMERERKQREQMLLLKAAQMKRFEREKLNRINRAREQGWRHVLSSSGGSSPERKFYGGGGGNGAPHSPGISPFPVPGPSPVPSSALAPSRAPYDRIHPSAEKLTKPTPKDGAGEGAVHAGDNGRTSSDSGTLNGHACIPVKNGVRKEQRKTSLQNEQISKIDYANRFRGQLAVEKSKQVEEFLQRKREAMLNKVRAEGQLGARQNLAAIFASWTNSSRCRRPRVNKEEEEYLTRLRQIRLQNFNERQQIKARLRGEKYDSDGSDSQESCEEAVLRMKKIEALKAQSKARAAVLKEQLEKKRREAYEREKKAWEDHQLVARGVKVAVGPVGGTPSPAAPPTPPSDPTPQEAPSKATPSSRPSTPAISMTAALKDVGAISSGQSSPMKEDSLKVESSDVQSDKKEILRRLNQKVQTTEEVSVKEPTPSSNQPCPDPQPVQPSITEERPSTAADRKKWEAVAPPILSVAEQTLEETCATLAVSQVASPETNTPSCGDRKKWQTDGVPLLSVAQQTLEETSFRTAGQLTEKTVGEVICMDAWQGEGLRKAWGQSPDSEVLKVLEEAELQSFTQRLDPASTCDQTLTESLNPIEESDATNEAKQVPPPEPCQPATDHDKQEVTCQETVPIIEEVKSQELIQTTAEGTEKSHSAESDIEVVDDMESVVLDDVPQKTAEPPPAEVIQAWTKDTPESLPAEGRVIQAECTEVPQREEKHPEPPADESLFVKLSSSPAHRRTVALAMLSAQSSLEDSSSAASRSRSVSPLRSHRHEDALLIGLSTGLFDANNPKMLRTCSLPDLSKVFSDATGPEGAAAPDSNLEIEDLEDKADEQSEPEDVYEDDDLRELRASMERLLQEQHSEDDDDEEEDEGGGSNGSPADEEPAGLNGLASAGDDKRSPEEETFNGMAEDDEDVSHGEEEPGGPVTNGMEFEDDEEQNSSEAQLNEEWQSDGSEEEDDEELEQQDSIFSRLEELRLHLEQAMGFENFIQAYNKIKAIHEDEDENIAMGSSMVQNILGTEHQHLYPKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_009294677.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio NIMA-related kinase 1 (nek1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009294677.2

ORF Insert Sequence:

ATGGATAAAT ATGAGAGGTT AAAGAAGATT GGGGAGGGAT CCTTTGGGAA AGCCATCTTG 
GTCAAGTCAA GGACAGATGG CCGACAATAT GTTATCAAGG AGATTGGTAT CTCCAGGATG 
TCGAACAAGG AACGACAGGA ATCACGTAAA GAGGTGGCAG TTCTGGCTAA CATGAGCCAT 
CCAAACATTG TCCAATACAA GGAATCCTTT GAAGAGAGTG GTTGTTTGTA CATTGTAATG 
GACTACTGTG AGGGAGGAGA CCTGTTTAAG AAGATCAATA ATCAAAGAGG ATCTCTTTTC 
CCAGAGGAAC AGATTCTTGA CTGGTTTGTG CAAATCTGTC TGGCTCTTAA ACATGTACAC 
GACCGCAAAA TCCTACATAG GGACATCAAA TCACAGAATA TTTTTCTGAC CAAAGATGGT 
ACTGTGCAGC TTGGAGACTT TGGAATCGCT AGAGTTTTGA ACAGCACCGT GGAGTTGGCA 
AGGACATGTA TCGGCACTCC TTATTACCTG TCGCCAGAGA TATGTGAAAA TAAACCATAC 
AACAACAAGA GTGATATCTG GGCCCTGGGA TGTGTCCTGT ATGAAATGTG CACGCTTAAG 
CATGCATTTG AAGCAGGAAA CATGAAGAAC CTGGTGCTGA AAATCATCCG TGGTTCATAC 
CCGCCTGTTT CCATCCATTA CTCCCCAGAT TTGCGCTCTC TCCTGGCCCA GCTCTTCAAG 
CGTAACCCCC GGGAGAGACC CTCCGTCTCG ACCATTTTGG ACAAGCCTTT TCTTGCTCGC 
AGAATTCACA AGTTTCTCAG CCCTCAGCTT ATTGCTCAGG AATTCAGCCA TTCAATTCAC 
CTTCAGCCCA AAATGAGTGT GGCCCATGCA GCACCAGCCA AACGTCCAGC CCCAGGCCCT 
ATCCCAATCA CCTCAGCTCA GAAAATCACC AAACCAGCAG CCAAATACGG AGTGCCTTTA 
ACAGTGCGGA GACCATCTGA AGTCGCCAGG AAGGTCCCAG ATGCAGCAAA GAAACCAGTT 
AAACTTAAGC AGGCTCCCCC TCCTGCAGCG GTTCAGAGGA GAGTGAGTCG AGTGGAGGAG 
GAGAGGAGAA AGCATGAGGA AGGAGTGAGG AAGAAGCGGA TGGAGATGAT GGAAAGAGAG 
AGAAAACAAA GAGAACAGAT GTTGTTACTG AAGGCTGCTC AGATGAAGAG ATTTGAGAGA 
GAAAAGCTGA ACCGAATCAA CCGAGCTCGA GAGCAGGGCT GGAGACACGT GCTGAGTTCT 
AGTGGAGGAA GCAGTCCTGA GCGGAAGTTT TATGGAGGTG GCGGCGGCAA TGGAGCTCCC 
CATTCCCCAG GAATATCTCC CTTTCCTGTG CCTGGACCCT CCCCTGTTCC TTCTTCAGCT 
TTAGCTCCAA GCAGAGCGCC ATATGACCGC ATCCACCCTT CAGCTGAAAA ATTAACCAAA 
CCTACACCTA AAGATGGTGC TGGTGAAGGA GCCGTGCATG CAGGGGACAA TGGCAGGACT 
TCTTCAGACT CAGGGACTCT AAATGGTCAT GCCTGCATTC CTGTTAAGAA TGGCGTCAGG 
AAAGAACAAC GTAAAACAAG TCTCCAAAAT GAGCAGATTT CAAAGATCGA CTATGCCAAT 
AGGTTTCGAG GTCAGCTGGC AGTAGAGAAA TCCAAGCAGG TGGAGGAGTT TCTACAGCGG 
AAAAGAGAAG CCATGCTGAA TAAAGTTCGT GCAGAAGGAC AGCTGGGGGC CCGGCAAAAC 
CTGGCCGCCA TCTTTGCAAG CTGGACAAAC TCCTCCCGAT GCAGGAGACC CCGAGTGAAC 
AAAGAGGAGG AGGAGTATTT GACCAGATTA CGACAGATCA GACTACAGAA CTTTAATGAA 
CGTCAGCAGA TTAAAGCTCG GCTCAGAGGG GAGAAGTATG ACAGTGATGG CTCAGACAGT 
CAGGAGTCAT GTGAAGAGGC AGTGCTCAGG ATGAAGAAGA TTGAGGCTTT GAAGGCCCAG 
TCAAAGGCTC GTGCTGCTGT ACTGAAAGAG CAGCTGGAAA AGAAGAGAAG AGAAGCGTAT 
GAAAGAGAGA AGAAAGCATG GGAGGATCAT CAGCTTGTCG CTCGAGGAGT AAAAGTTGCA 
GTTGGGCCTG TGGGTGGGAC TCCCTCTCCT GCAGCCCCTC CAACTCCACC ATCTGACCCC 
ACCCCTCAGG AAGCTCCTTC TAAAGCCACA CCCTCCTCTA GACCCAGCAC TCCAGCCATC 
TCTATGACTG CTGCACTGAA GGATGTGGGC GCGATTTCAT CAGGGCAGAG TTCTCCTATG 
AAAGAGGACT CCCTTAAAGT TGAATCATCA GATGTACAGA GTGACAAGAA GGAGATTCTT 
CGCAGACTTA ATCAGAAGGT TCAGACCACT GAGGAAGTTT CTGTAAAAGA GCCCACACCC 
TCCAGCAATC AACCATGTCC TGATCCTCAG CCAGTCCAAC CCTCCATCAC AGAGGAAAGG 
CCTTCCACAG CTGCAGATAG GAAGAAGTGG GAAGCCGTAG CTCCACCCAT ACTGTCTGTT 
GCTGAACAGA CTCTCGAGGA AACCTGTGCT ACTCTGGCAG TGTCTCAGGT GGCATCACCA 
GAAACGAATA CTCCCTCGTG TGGAGACAGG AAGAAATGGC AGACAGATGG TGTTCCTCTT 
CTGTCTGTGG CTCAGCAGAC ACTGGAAGAA ACCAGCTTTA GAACAGCTGG TCAATTAACA 
GAAAAAACAG TAGGTGAAGT CATTTGCATG GATGCCTGGC AGGGGGAGGG GCTAAGGAAG 
GCTTGGGGGC AGAGTCCAGA TTCAGAGGTT TTGAAGGTGT TGGAAGAGGC AGAACTTCAG 
TCATTTACTC AGAGACTAGA TCCTGCCAGC ACGTGTGACC AAACACTGAC TGAAAGCCTT 
AATCCCATTG AGGAATCGGA TGCCACTAAT GAAGCCAAGC AAGTGCCTCC TCCTGAACCG 
TGCCAGCCTG CCACTGACCA TGACAAACAA GAAGTGACAT GTCAAGAGAC AGTGCCAATC 
ATAGAAGAGG TTAAAAGTCA GGAACTTATC CAAACAACAG CAGAGGGGAC AGAAAAATCT 
CATTCTGCAG AAAGTGACAT AGAGGTGGTG GATGATATGG AGTCAGTTGT TCTGGATGAT 
GTACCACAGA AAACCGCAGA GCCGCCCCCT GCAGAAGTAA TTCAGGCGTG GACTAAAGAC 
ACTCCAGAGT CTCTGCCAGC AGAGGGCAGA GTAATACAAG CAGAATGCAC AGAGGTACCT 
CAGAGAGAAG AGAAACATCC TGAACCTCCA GCAGATGAGT CTTTGTTTGT GAAGTTAAGC 
AGTAGTCCAG CTCACAGACG CACAGTAGCG TTGGCCATGC TGTCTGCTCA ATCCTCATTG 
GAAGACTCTT CCTCTGCTGC CTCACGTTCT CGATCAGTGT CTCCACTGCG CTCCCACCGC 
CATGAAGATG CACTGCTCAT CGGCCTCTCC ACTGGCCTCT TCGATGCAAA TAACCCAAAG 
ATGCTAAGGA CGTGCTCCTT ACCAGACCTC AGTAAGGTGT TTAGTGATGC TACAGGACCT 
GAAGGTGCAG CCGCTCCTGA CAGCAACCTA GAAATTGAAG ATCTGGAAGA TAAAGCAGAT 
GAGCAGTCTG AACCTGAGGA TGTGTATGAG GATGACGATT TAAGAGAGCT CAGAGCATCT 
ATGGAGAGGC TTCTTCAGGA GCAGCACAGT GAAGATGATG ATGATGAAGA AGAGGATGAG 
GGTGGTGGAT CTAATGGCAG CCCGGCCGAT GAAGAGCCAG CTGGTCTTAA TGGGTTAGCT 
AGTGCTGGTG ATGACAAACG CAGCCCAGAG GAAGAGACTT TTAATGGAAT GGCTGAAGAT 
GATGAGGATG TTAGCCATGG TGAAGAAGAA CCAGGAGGCC CAGTCACAAA TGGAATGGAA 
TTTGAAGATG ATGAAGAGCA GAACAGCAGT GAAGCACAGC TCAATGAGGA GTGGCAGTCA 
GATGGCAGCG AGGAGGAGGA CGACGAAGAG CTGGAACAGC AGGACAGCAT CTTCAGTCGT 
CTGGAGGAGC TGCGGCTTCA TCTAGAGCAG GCCATGGGCT TTGAGAACTT CATCCAGGCC 
TACAACAAGA TCAAGGCTAT TCATGAGGAT GAGGATGAAA ACATTGCAAT GGGCTCCAGC 
ATGGTGCAAA ACATTTTGGG AACTGAGCAC CAGCACTTGT ATCCTAAAAT CCTCCATTTG 
GTGATGGCAG ATGGAGCTTA CCAGGAAGAT AATGATGAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_009294678.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009292953.1 Danio rerio NIMA-related kinase 1 (nek1), transcript variant X2, mRNA.

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CloneID	ODa42283
Clone ID Related Accession (Same CDS sequence)	XM_009294678.3 , XM_009294678.2
Accession Version	XM_009294678.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4239bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase Nek1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009294678.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAAAT ATGAGAGGTT AAAGAAGATT GGGGAGGGAT CCTTTGGGAA AGCCATCTTG 
GTCAAGTCAA GGACAGATGG CCGACAATAT GTTATCAAGG AGATTGGTAT CTCCAGGATG 
TCGAACAAGG AACGACAGGA ATCACGTAAA GAGGTGGCAG TTCTGGCTAA CATGAGCCAT 
CCAAACATTG TCCAATACAA GGAATCCTTT GAAGAGAGTG GTTGTTTGTA CATTGTAATG 
GACTACTGTG AGGGAGGAGA CCTGTTTAAG AAGATCAATA ATCAAAGAGG ATCTCTTTTC 
CCAGAGGAAC AGATTCTTGA CTGGTTTGTG CAAATCTGTC TGGCTCTTAA ACATGTACAC 
GACCGCAAAA TCCTACATAG GGACATCAAA TCACAGAATA TTTTTCTGAC CAAAGATGGT 
ACTGTGCAGC TTGGAGACTT TGGAATCGCT AGAGTTTTGA ACAGCACCGT GGAGTTGGCA 
AGGACATGTA TCGGCACTCC TTATTACCTG TCGCCAGAGA TATGTGAAAA TAAACCATAC 
AACAACAAGA GTGATATCTG GGCCCTGGGA TGTGTCCTGT ATGAAATGTG CACGCTTAAG 
CATGCATTTG AAGCAGGAAA CATGAAGAAC CTGGTGCTGA AAATCATCCG TGGTTCATAC 
CCGCCTGTTT CCATCCATTA CTCCCCAGAT TTGCGCTCTC TCCTGGCCCA GCTCTTCAAG 
CGTAACCCCC GGGAGAGACC CTCCGTCTCG ACCATTTTGG ACAAGCCTTT TCTTGCTCGC 
AGAATTCACA AGTTTCTCAG CCCTCAGCTT ATTGCTCAGG AATTCAGCCA TTCAATTCAC 
CTTCAGCCCA AAATGAGTGT GGCCCATGCA GCACCAGCCA AACGTCCAGC CCCAGGCCCT 
ATCCCAATCA CCTCAGCTCA GAAAATCACC AAACCAGCAG CCAAATACGG AGTGCCTTTA 
ACAGTGCGGA GACCATCTGA AGTCGCCAGG AAGGTCCCAG ATGCAGCAAA GAAACCAGTT 
AAACTTAAGC AGGCTCCCCC TCCTGCAGCG GTTCAGAGGA GAGTGAGTCG AGTGGAGGAG 
GAGAGGAGAA AGCATGAGGA AGGAGTGAGG AAGAAGCGGA TGGAGATGAT GGAAAGAGAG 
AGAAAACAAA GAGAACAGAT GTTGTTACTG AAGGCTGCTC AGATGAAGAG ATTTGAGAGA 
GAAAAGCTGA ACCGAATCAA CCGAGCTCGA GAGCAGGGCT GGAGACACGT GCTGAGTTCT 
AGTGGAGGAA GCAGTCCTGA GCGGAAGTTT TATGGAGGTG GCGGCGGCAA TGGAGCTCCC 
CATTCCCCAG GAATATCTCC CTTTCCTGTG CCTGGACCCT CCCCTGTTCC TTCTTCAGCT 
TTAGCTCCAA GCAGAGCGCC ATATGACCGC ATCCACCCTT CAGCTGAAAA ATTAACCAAA 
CCTACACCTA AAGATGGTGC TGGTGAAGGA GCCGTGCATG CAGGGGACAA TGGCAGGACT 
TCTTCAGACT CAGGGACTCT AAATGGTCAT GCCTGCATTC CTGTTAAGAA TGGCGTCAGG 
AAAGAACAAC GTAAAACAAG TCTCCAAAAT GAGCAGATTT CAAAGATCGA CTATGCCAAT 
AGGTTTCGAG GTCAGCTGGC AGTAGAGAAA TCCAAGCAGG TGGAGGAGTT TCTACAGCGG 
AAAAGAGAAG CCATGCTGAA TAAAGTTCGT GCAGAAGGAC AGCTGGGGGC CCGGCAAAAC 
CTGGCCGCCA TCTTTGCAAG CTGGACAAAC TCCTCCCGAT GCAGGAGACC CCGAGTGAAC 
AAAGAGGAGG AGGAGTATTT GACCAGATTA CGACAGATCA GACTACAGAA CTTTAATGAA 
CGTCAGCAGA TTAAAGCTCG GCTCAGAGGG GAGAAGTATG ACAGTGATGG CTCAGACAGT 
CAGGAGTCAT GTGAAGAGGC AGTGCTCAGG ATGAAGAAGA TTGAGGCTTT GAAGGCCCAG 
TCAAAGGCTC GTGCTGCTGT ACTGAAAGAG CAGCTGGAAA AGAAGAGAAG AGAAGCGTAT 
GAAAGAGAGA AGAAAGCATG GGAGGATCAT CTTGTCGCTC GAGGAGTAAA AGTTGCAGTT 
GGGCCTGTGG GTGGGACTCC CTCTCCTGCA GCCCCTCCAA CTCCACCATC TGACCCCACC 
CCTCAGGAAG CTCCTTCTAA AGCCACACCC TCCTCTAGAC CCAGCACTCC AGCCATCTCT 
ATGACTGCTG CACTGAAGGA TGTGGGCGCG ATTTCATCAG GGCAGAGTTC TCCTATGAAA 
GAGGACTCCC TTAAAGTTGA ATCATCAGAT GTACAGAGTG ACAAGAAGGA GATTCTTCGC 
AGACTTAATC AGAAGGTTCA GACCACTGAG GAAGTTTCTG TAAAAGAGCC CACACCCTCC 
AGCAATCAAC CATGTCCTGA TCCTCAGCCA GTCCAACCCT CCATCACAGA GGAAAGGCCT 
TCCACAGCTG CAGATAGGAA GAAGTGGGAA GCCGTAGCTC CACCCATACT GTCTGTTGCT 
GAACAGACTC TCGAGGAAAC CTGTGCTACT CTGGCAGTGT CTCAGGTGGC ATCACCAGAA 
ACGAATACTC CCTCGTGTGG AGACAGGAAG AAATGGCAGA CAGATGGTGT TCCTCTTCTG 
TCTGTGGCTC AGCAGACACT GGAAGAAACC AGCTTTAGAA CAGCTGGTCA ATTAACAGAA 
AAAACAGTAG GTGAAGTCAT TTGCATGGAT GCCTGGCAGG GGGAGGGGCT AAGGAAGGCT 
TGGGGGCAGA GTCCAGATTC AGAGGTTTTG AAGGTGTTGG AAGAGGCAGA ACTTCAGTCA 
TTTACTCAGA GACTAGATCC TGCCAGCACG TGTGACCAAA CACTGACTGA AAGCCTTAAT 
CCCATTGAGG AATCGGATGC CACTAATGAA GCCAAGCAAG TGCCTCCTCC TGAACCGTGC 
CAGCCTGCCA CTGACCATGA CAAACAAGAA GTGACATGTC AAGAGACAGT GCCAATCATA 
GAAGAGGTTA AAAGTCAGGA ACTTATCCAA ACAACAGCAG AGGGGACAGA AAAATCTCAT 
TCTGCAGAAA GTGACATAGA GGTGGTGGAT GATATGGAGT CAGTTGTTCT GGATGATGTA 
CCACAGAAAA CCGCAGAGCC GCCCCCTGCA GAAGTAATTC AGGCGTGGAC TAAAGACACT 
CCAGAGTCTC TGCCAGCAGA GGGCAGAGTA ATACAAGCAG AATGCACAGA GGTACCTCAG 
AGAGAAGAGA AACATCCTGA ACCTCCAGCA GATGAGTCTT TGTTTGTGAA GTTAAGCAGT 
AGTCCAGCTC ACAGACGCAC AGTAGCGTTG GCCATGCTGT CTGCTCAATC CTCATTGGAA 
GACTCTTCCT CTGCTGCCTC ACGTTCTCGA TCAGTGTCTC CACTGCGCTC CCACCGCCAT 
GAAGATGCAC TGCTCATCGG CCTCTCCACT GGCCTCTTCG ATGCAAATAA CCCAAAGATG 
CTAAGGACGT GCTCCTTACC AGACCTCAGT AAGGTGTTTA GTGATGCTAC AGGACCTGAA 
GGTGCAGCCG CTCCTGACAG CAACCTAGAA ATTGAAGATC TGGAAGATAA AGCAGATGAG 
CAGTCTGAAC CTGAGGATGT GTATGAGGAT GACGATTTAA GAGAGCTCAG AGCATCTATG 
GAGAGGCTTC TTCAGGAGCA GCACAGTGAA GATGATGATG ATGAAGAAGA GGATGAGGGT 
GGTGGATCTA ATGGCAGCCC GGCCGATGAA GAGCCAGCTG GTCTTAATGG GTTAGCTAGT 
GCTGGTGATG ACAAACGCAG CCCAGAGGAA GAGACTTTTA ATGGAATGGC TGAAGATGAT 
GAGGATGTTA GCCATGGTGA AGAAGAACCA GGAGGCCCAG TCACAAATGG AATGGAATTT 
GAAGATGATG AAGAGCAGAA CAGCAGTGAA GCACAGCTCA ATGAGGAGTG GCAGTCAGAT 
GGCAGCGAGG AGGAGGACGA CGAAGAGCTG GAACAGCAGG ACAGCATCTT CAGTCGTCTG 
GAGGAGCTGC GGCTTCATCT AGAGCAGGCC ATGGGCTTTG AGAACTTCAT CCAGGCCTAC 
AACAAGATCA AGGCTATTCA TGAGGATGAG GATGAAAACA TTGCAATGGG CTCCAGCATG 
GTGCAAAACA TTTTGGGAAC TGAGCACCAG CACTTGTATC CTAAAATCCT CCATTTGGTG 
ATGGCAGATG GAGCTTACCA GGAAGATAAT GATGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009292953.1
CDS	127..4365
Translation	MDKYERLKKIGEGSFGKAILVKSRTDGRQYVIKEIGISRMSNKERQESRKEVAVLANMSHPNIVQYKESFEESGCLYIVMDYCEGGDLFKKINNQRGSLFPEEQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIIRGSYPPVSIHYSPDLRSLLAQLFKRNPRERPSVSTILDKPFLARRIHKFLSPQLIAQEFSHSIHLQPKMSVAHAAPAKRPAPGPIPITSAQKITKPAAKYGVPLTVRRPSEVARKVPDAAKKPVKLKQAPPPAAVQRRVSRVEEERRKHEEGVRKKRMEMMERERKQREQMLLLKAAQMKRFEREKLNRINRAREQGWRHVLSSSGGSSPERKFYGGGGGNGAPHSPGISPFPVPGPSPVPSSALAPSRAPYDRIHPSAEKLTKPTPKDGAGEGAVHAGDNGRTSSDSGTLNGHACIPVKNGVRKEQRKTSLQNEQISKIDYANRFRGQLAVEKSKQVEEFLQRKREAMLNKVRAEGQLGARQNLAAIFASWTNSSRCRRPRVNKEEEEYLTRLRQIRLQNFNERQQIKARLRGEKYDSDGSDSQESCEEAVLRMKKIEALKAQSKARAAVLKEQLEKKRREAYEREKKAWEDHLVARGVKVAVGPVGGTPSPAAPPTPPSDPTPQEAPSKATPSSRPSTPAISMTAALKDVGAISSGQSSPMKEDSLKVESSDVQSDKKEILRRLNQKVQTTEEVSVKEPTPSSNQPCPDPQPVQPSITEERPSTAADRKKWEAVAPPILSVAEQTLEETCATLAVSQVASPETNTPSCGDRKKWQTDGVPLLSVAQQTLEETSFRTAGQLTEKTVGEVICMDAWQGEGLRKAWGQSPDSEVLKVLEEAELQSFTQRLDPASTCDQTLTESLNPIEESDATNEAKQVPPPEPCQPATDHDKQEVTCQETVPIIEEVKSQELIQTTAEGTEKSHSAESDIEVVDDMESVVLDDVPQKTAEPPPAEVIQAWTKDTPESLPAEGRVIQAECTEVPQREEKHPEPPADESLFVKLSSSPAHRRTVALAMLSAQSSLEDSSSAASRSRSVSPLRSHRHEDALLIGLSTGLFDANNPKMLRTCSLPDLSKVFSDATGPEGAAAPDSNLEIEDLEDKADEQSEPEDVYEDDDLRELRASMERLLQEQHSEDDDDEEEDEGGGSNGSPADEEPAGLNGLASAGDDKRSPEEETFNGMAEDDEDVSHGEEEPGGPVTNGMEFEDDEEQNSSEAQLNEEWQSDGSEEEDDEELEQQDSIFSRLEELRLHLEQAMGFENFIQAYNKIKAIHEDEDENIAMGSSMVQNILGTEHQHLYPKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_009294678.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio NIMA-related kinase 1 (nek1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009294678.3

ORF Insert Sequence:

ATGGATAAAT ATGAGAGGTT AAAGAAGATT GGGGAGGGAT CCTTTGGGAA AGCCATCTTG 
GTCAAGTCAA GGACAGATGG CCGACAATAT GTTATCAAGG AGATTGGTAT CTCCAGGATG 
TCGAACAAGG AACGACAGGA ATCACGTAAA GAGGTGGCAG TTCTGGCTAA CATGAGCCAT 
CCAAACATTG TCCAATACAA GGAATCCTTT GAAGAGAGTG GTTGTTTGTA CATTGTAATG 
GACTACTGTG AGGGAGGAGA CCTGTTTAAG AAGATCAATA ATCAAAGAGG ATCTCTTTTC 
CCAGAGGAAC AGATTCTTGA CTGGTTTGTG CAAATCTGTC TGGCTCTTAA ACATGTACAC 
GACCGCAAAA TCCTACATAG GGACATCAAA TCACAGAATA TTTTTCTGAC CAAAGATGGT 
ACTGTGCAGC TTGGAGACTT TGGAATCGCT AGAGTTTTGA ACAGCACCGT GGAGTTGGCA 
AGGACATGTA TCGGCACTCC TTATTACCTG TCGCCAGAGA TATGTGAAAA TAAACCATAC 
AACAACAAGA GTGATATCTG GGCCCTGGGA TGTGTCCTGT ATGAAATGTG CACGCTTAAG 
CATGCATTTG AAGCAGGAAA CATGAAGAAC CTGGTGCTGA AAATCATCCG TGGTTCATAC 
CCGCCTGTTT CCATCCATTA CTCCCCAGAT TTGCGCTCTC TCCTGGCCCA GCTCTTCAAG 
CGTAACCCCC GGGAGAGACC CTCCGTCTCG ACCATTTTGG ACAAGCCTTT TCTTGCTCGC 
AGAATTCACA AGTTTCTCAG CCCTCAGCTT ATTGCTCAGG AATTCAGCCA TTCAATTCAC 
CTTCAGCCCA AAATGAGTGT GGCCCATGCA GCACCAGCCA AACGTCCAGC CCCAGGCCCT 
ATCCCAATCA CCTCAGCTCA GAAAATCACC AAACCAGCAG CCAAATACGG AGTGCCTTTA 
ACAGTGCGGA GACCATCTGA AGTCGCCAGG AAGGTCCCAG ATGCAGCAAA GAAACCAGTT 
AAACTTAAGC AGGCTCCCCC TCCTGCAGCG GTTCAGAGGA GAGTGAGTCG AGTGGAGGAG 
GAGAGGAGAA AGCATGAGGA AGGAGTGAGG AAGAAGCGGA TGGAGATGAT GGAAAGAGAG 
AGAAAACAAA GAGAACAGAT GTTGTTACTG AAGGCTGCTC AGATGAAGAG ATTTGAGAGA 
GAAAAGCTGA ACCGAATCAA CCGAGCTCGA GAGCAGGGCT GGAGACACGT GCTGAGTTCT 
AGTGGAGGAA GCAGTCCTGA GCGGAAGTTT TATGGAGGTG GCGGCGGCAA TGGAGCTCCC 
CATTCCCCAG GAATATCTCC CTTTCCTGTG CCTGGACCCT CCCCTGTTCC TTCTTCAGCT 
TTAGCTCCAA GCAGAGCGCC ATATGACCGC ATCCACCCTT CAGCTGAAAA ATTAACCAAA 
CCTACACCTA AAGATGGTGC TGGTGAAGGA GCCGTGCATG CAGGGGACAA TGGCAGGACT 
TCTTCAGACT CAGGGACTCT AAATGGTCAT GCCTGCATTC CTGTTAAGAA TGGCGTCAGG 
AAAGAACAAC GTAAAACAAG TCTCCAAAAT GAGCAGATTT CAAAGATCGA CTATGCCAAT 
AGGTTTCGAG GTCAGCTGGC AGTAGAGAAA TCCAAGCAGG TGGAGGAGTT TCTACAGCGG 
AAAAGAGAAG CCATGCTGAA TAAAGTTCGT GCAGAAGGAC AGCTGGGGGC CCGGCAAAAC 
CTGGCCGCCA TCTTTGCAAG CTGGACAAAC TCCTCCCGAT GCAGGAGACC CCGAGTGAAC 
AAAGAGGAGG AGGAGTATTT GACCAGATTA CGACAGATCA GACTACAGAA CTTTAATGAA 
CGTCAGCAGA TTAAAGCTCG GCTCAGAGGG GAGAAGTATG ACAGTGATGG CTCAGACAGT 
CAGGAGTCAT GTGAAGAGGC AGTGCTCAGG ATGAAGAAGA TTGAGGCTTT GAAGGCCCAG 
TCAAAGGCTC GTGCTGCTGT ACTGAAAGAG CAGCTGGAAA AGAAGAGAAG AGAAGCGTAT 
GAAAGAGAGA AGAAAGCATG GGAGGATCAT CTTGTCGCTC GAGGAGTAAA AGTTGCAGTT 
GGGCCTGTGG GTGGGACTCC CTCTCCTGCA GCCCCTCCAA CTCCACCATC TGACCCCACC 
CCTCAGGAAG CTCCTTCTAA AGCCACACCC TCCTCTAGAC CCAGCACTCC AGCCATCTCT 
ATGACTGCTG CACTGAAGGA TGTGGGCGCG ATTTCATCAG GGCAGAGTTC TCCTATGAAA 
GAGGACTCCC TTAAAGTTGA ATCATCAGAT GTACAGAGTG ACAAGAAGGA GATTCTTCGC 
AGACTTAATC AGAAGGTTCA GACCACTGAG GAAGTTTCTG TAAAAGAGCC CACACCCTCC 
AGCAATCAAC CATGTCCTGA TCCTCAGCCA GTCCAACCCT CCATCACAGA GGAAAGGCCT 
TCCACAGCTG CAGATAGGAA GAAGTGGGAA GCCGTAGCTC CACCCATACT GTCTGTTGCT 
GAACAGACTC TCGAGGAAAC CTGTGCTACT CTGGCAGTGT CTCAGGTGGC ATCACCAGAA 
ACGAATACTC CCTCGTGTGG AGACAGGAAG AAATGGCAGA CAGATGGTGT TCCTCTTCTG 
TCTGTGGCTC AGCAGACACT GGAAGAAACC AGCTTTAGAA CAGCTGGTCA ATTAACAGAA 
AAAACAGTAG GTGAAGTCAT TTGCATGGAT GCCTGGCAGG GGGAGGGGCT AAGGAAGGCT 
TGGGGGCAGA GTCCAGATTC AGAGGTTTTG AAGGTGTTGG AAGAGGCAGA ACTTCAGTCA 
TTTACTCAGA GACTAGATCC TGCCAGCACG TGTGACCAAA CACTGACTGA AAGCCTTAAT 
CCCATTGAGG AATCGGATGC CACTAATGAA GCCAAGCAAG TGCCTCCTCC TGAACCGTGC 
CAGCCTGCCA CTGACCATGA CAAACAAGAA GTGACATGTC AAGAGACAGT GCCAATCATA 
GAAGAGGTTA AAAGTCAGGA ACTTATCCAA ACAACAGCAG AGGGGACAGA AAAATCTCAT 
TCTGCAGAAA GTGACATAGA GGTGGTGGAT GATATGGAGT CAGTTGTTCT GGATGATGTA 
CCACAGAAAA CCGCAGAGCC GCCCCCTGCA GAAGTAATTC AGGCGTGGAC TAAAGACACT 
CCAGAGTCTC TGCCAGCAGA GGGCAGAGTA ATACAAGCAG AATGCACAGA GGTACCTCAG 
AGAGAAGAGA AACATCCTGA ACCTCCAGCA GATGAGTCTT TGTTTGTGAA GTTAAGCAGT 
AGTCCAGCTC ACAGACGCAC AGTAGCGTTG GCCATGCTGT CTGCTCAATC CTCATTGGAA 
GACTCTTCCT CTGCTGCCTC ACGTTCTCGA TCAGTGTCTC CACTGCGCTC CCACCGCCAT 
GAAGATGCAC TGCTCATCGG CCTCTCCACT GGCCTCTTCG ATGCAAATAA CCCAAAGATG 
CTAAGGACGT GCTCCTTACC AGACCTCAGT AAGGTGTTTA GTGATGCTAC AGGACCTGAA 
GGTGCAGCCG CTCCTGACAG CAACCTAGAA ATTGAAGATC TGGAAGATAA AGCAGATGAG 
CAGTCTGAAC CTGAGGATGT GTATGAGGAT GACGATTTAA GAGAGCTCAG AGCATCTATG 
GAGAGGCTTC TTCAGGAGCA GCACAGTGAA GATGATGATG ATGAAGAAGA GGATGAGGGT 
GGTGGATCTA ATGGCAGCCC GGCCGATGAA GAGCCAGCTG GTCTTAATGG GTTAGCTAGT 
GCTGGTGATG ACAAACGCAG CCCAGAGGAA GAGACTTTTA ATGGAATGGC TGAAGATGAT 
GAGGATGTTA GCCATGGTGA AGAAGAACCA GGAGGCCCAG TCACAAATGG AATGGAATTT 
GAAGATGATG AAGAGCAGAA CAGCAGTGAA GCACAGCTCA ATGAGGAGTG GCAGTCAGAT 
GGCAGCGAGG AGGAGGACGA CGAAGAGCTG GAACAGCAGG ACAGCATCTT CAGTCGTCTG 
GAGGAGCTGC GGCTTCATCT AGAGCAGGCC ATGGGCTTTG AGAACTTCAT CCAGGCCTAC 
AACAAGATCA AGGCTATTCA TGAGGATGAG GATGAAAACA TTGCAATGGG CTCCAGCATG 
GTGCAAAACA TTTTGGGAAC TGAGCACCAG CACTTGTATC CTAAAATCCT CCATTTGGTG 
ATGGCAGATG GAGCTTACCA GGAAGATAAT GATGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_009294678.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009292953.1 Danio rerio NIMA-related kinase 1 (nek1), transcript variant X2, mRNA.

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CloneID	ODa42283
Clone ID Related Accession (Same CDS sequence)	XM_009294678.3 , XM_009294678.2
Accession Version	XM_009294678.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4239bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	serine/threonine-protein kinase Nek1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009294678.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAAAT ATGAGAGGTT AAAGAAGATT GGGGAGGGAT CCTTTGGGAA AGCCATCTTG 
GTCAAGTCAA GGACAGATGG CCGACAATAT GTTATCAAGG AGATTGGTAT CTCCAGGATG 
TCGAACAAGG AACGACAGGA ATCACGTAAA GAGGTGGCAG TTCTGGCTAA CATGAGCCAT 
CCAAACATTG TCCAATACAA GGAATCCTTT GAAGAGAGTG GTTGTTTGTA CATTGTAATG 
GACTACTGTG AGGGAGGAGA CCTGTTTAAG AAGATCAATA ATCAAAGAGG ATCTCTTTTC 
CCAGAGGAAC AGATTCTTGA CTGGTTTGTG CAAATCTGTC TGGCTCTTAA ACATGTACAC 
GACCGCAAAA TCCTACATAG GGACATCAAA TCACAGAATA TTTTTCTGAC CAAAGATGGT 
ACTGTGCAGC TTGGAGACTT TGGAATCGCT AGAGTTTTGA ACAGCACCGT GGAGTTGGCA 
AGGACATGTA TCGGCACTCC TTATTACCTG TCGCCAGAGA TATGTGAAAA TAAACCATAC 
AACAACAAGA GTGATATCTG GGCCCTGGGA TGTGTCCTGT ATGAAATGTG CACGCTTAAG 
CATGCATTTG AAGCAGGAAA CATGAAGAAC CTGGTGCTGA AAATCATCCG TGGTTCATAC 
CCGCCTGTTT CCATCCATTA CTCCCCAGAT TTGCGCTCTC TCCTGGCCCA GCTCTTCAAG 
CGTAACCCCC GGGAGAGACC CTCCGTCTCG ACCATTTTGG ACAAGCCTTT TCTTGCTCGC 
AGAATTCACA AGTTTCTCAG CCCTCAGCTT ATTGCTCAGG AATTCAGCCA TTCAATTCAC 
CTTCAGCCCA AAATGAGTGT GGCCCATGCA GCACCAGCCA AACGTCCAGC CCCAGGCCCT 
ATCCCAATCA CCTCAGCTCA GAAAATCACC AAACCAGCAG CCAAATACGG AGTGCCTTTA 
ACAGTGCGGA GACCATCTGA AGTCGCCAGG AAGGTCCCAG ATGCAGCAAA GAAACCAGTT 
AAACTTAAGC AGGCTCCCCC TCCTGCAGCG GTTCAGAGGA GAGTGAGTCG AGTGGAGGAG 
GAGAGGAGAA AGCATGAGGA AGGAGTGAGG AAGAAGCGGA TGGAGATGAT GGAAAGAGAG 
AGAAAACAAA GAGAACAGAT GTTGTTACTG AAGGCTGCTC AGATGAAGAG ATTTGAGAGA 
GAAAAGCTGA ACCGAATCAA CCGAGCTCGA GAGCAGGGCT GGAGACACGT GCTGAGTTCT 
AGTGGAGGAA GCAGTCCTGA GCGGAAGTTT TATGGAGGTG GCGGCGGCAA TGGAGCTCCC 
CATTCCCCAG GAATATCTCC CTTTCCTGTG CCTGGACCCT CCCCTGTTCC TTCTTCAGCT 
TTAGCTCCAA GCAGAGCGCC ATATGACCGC ATCCACCCTT CAGCTGAAAA ATTAACCAAA 
CCTACACCTA AAGATGGTGC TGGTGAAGGA GCCGTGCATG CAGGGGACAA TGGCAGGACT 
TCTTCAGACT CAGGGACTCT AAATGGTCAT GCCTGCATTC CTGTTAAGAA TGGCGTCAGG 
AAAGAACAAC GTAAAACAAG TCTCCAAAAT GAGCAGATTT CAAAGATCGA CTATGCCAAT 
AGGTTTCGAG GTCAGCTGGC AGTAGAGAAA TCCAAGCAGG TGGAGGAGTT TCTACAGCGG 
AAAAGAGAAG CCATGCTGAA TAAAGTTCGT GCAGAAGGAC AGCTGGGGGC CCGGCAAAAC 
CTGGCCGCCA TCTTTGCAAG CTGGACAAAC TCCTCCCGAT GCAGGAGACC CCGAGTGAAC 
AAAGAGGAGG AGGAGTATTT GACCAGATTA CGACAGATCA GACTACAGAA CTTTAATGAA 
CGTCAGCAGA TTAAAGCTCG GCTCAGAGGG GAGAAGTATG ACAGTGATGG CTCAGACAGT 
CAGGAGTCAT GTGAAGAGGC AGTGCTCAGG ATGAAGAAGA TTGAGGCTTT GAAGGCCCAG 
TCAAAGGCTC GTGCTGCTGT ACTGAAAGAG CAGCTGGAAA AGAAGAGAAG AGAAGCGTAT 
GAAAGAGAGA AGAAAGCATG GGAGGATCAT CTTGTCGCTC GAGGAGTAAA AGTTGCAGTT 
GGGCCTGTGG GTGGGACTCC CTCTCCTGCA GCCCCTCCAA CTCCACCATC TGACCCCACC 
CCTCAGGAAG CTCCTTCTAA AGCCACACCC TCCTCTAGAC CCAGCACTCC AGCCATCTCT 
ATGACTGCTG CACTGAAGGA TGTGGGCGCG ATTTCATCAG GGCAGAGTTC TCCTATGAAA 
GAGGACTCCC TTAAAGTTGA ATCATCAGAT GTACAGAGTG ACAAGAAGGA GATTCTTCGC 
AGACTTAATC AGAAGGTTCA GACCACTGAG GAAGTTTCTG TAAAAGAGCC CACACCCTCC 
AGCAATCAAC CATGTCCTGA TCCTCAGCCA GTCCAACCCT CCATCACAGA GGAAAGGCCT 
TCCACAGCTG CAGATAGGAA GAAGTGGGAA GCCGTAGCTC CACCCATACT GTCTGTTGCT 
GAACAGACTC TCGAGGAAAC CTGTGCTACT CTGGCAGTGT CTCAGGTGGC ATCACCAGAA 
ACGAATACTC CCTCGTGTGG AGACAGGAAG AAATGGCAGA CAGATGGTGT TCCTCTTCTG 
TCTGTGGCTC AGCAGACACT GGAAGAAACC AGCTTTAGAA CAGCTGGTCA ATTAACAGAA 
AAAACAGTAG GTGAAGTCAT TTGCATGGAT GCCTGGCAGG GGGAGGGGCT AAGGAAGGCT 
TGGGGGCAGA GTCCAGATTC AGAGGTTTTG AAGGTGTTGG AAGAGGCAGA ACTTCAGTCA 
TTTACTCAGA GACTAGATCC TGCCAGCACG TGTGACCAAA CACTGACTGA AAGCCTTAAT 
CCCATTGAGG AATCGGATGC CACTAATGAA GCCAAGCAAG TGCCTCCTCC TGAACCGTGC 
CAGCCTGCCA CTGACCATGA CAAACAAGAA GTGACATGTC AAGAGACAGT GCCAATCATA 
GAAGAGGTTA AAAGTCAGGA ACTTATCCAA ACAACAGCAG AGGGGACAGA AAAATCTCAT 
TCTGCAGAAA GTGACATAGA GGTGGTGGAT GATATGGAGT CAGTTGTTCT GGATGATGTA 
CCACAGAAAA CCGCAGAGCC GCCCCCTGCA GAAGTAATTC AGGCGTGGAC TAAAGACACT 
CCAGAGTCTC TGCCAGCAGA GGGCAGAGTA ATACAAGCAG AATGCACAGA GGTACCTCAG 
AGAGAAGAGA AACATCCTGA ACCTCCAGCA GATGAGTCTT TGTTTGTGAA GTTAAGCAGT 
AGTCCAGCTC ACAGACGCAC AGTAGCGTTG GCCATGCTGT CTGCTCAATC CTCATTGGAA 
GACTCTTCCT CTGCTGCCTC ACGTTCTCGA TCAGTGTCTC CACTGCGCTC CCACCGCCAT 
GAAGATGCAC TGCTCATCGG CCTCTCCACT GGCCTCTTCG ATGCAAATAA CCCAAAGATG 
CTAAGGACGT GCTCCTTACC AGACCTCAGT AAGGTGTTTA GTGATGCTAC AGGACCTGAA 
GGTGCAGCCG CTCCTGACAG CAACCTAGAA ATTGAAGATC TGGAAGATAA AGCAGATGAG 
CAGTCTGAAC CTGAGGATGT GTATGAGGAT GACGATTTAA GAGAGCTCAG AGCATCTATG 
GAGAGGCTTC TTCAGGAGCA GCACAGTGAA GATGATGATG ATGAAGAAGA GGATGAGGGT 
GGTGGATCTA ATGGCAGCCC GGCCGATGAA GAGCCAGCTG GTCTTAATGG GTTAGCTAGT 
GCTGGTGATG ACAAACGCAG CCCAGAGGAA GAGACTTTTA ATGGAATGGC TGAAGATGAT 
GAGGATGTTA GCCATGGTGA AGAAGAACCA GGAGGCCCAG TCACAAATGG AATGGAATTT 
GAAGATGATG AAGAGCAGAA CAGCAGTGAA GCACAGCTCA ATGAGGAGTG GCAGTCAGAT 
GGCAGCGAGG AGGAGGACGA CGAAGAGCTG GAACAGCAGG ACAGCATCTT CAGTCGTCTG 
GAGGAGCTGC GGCTTCATCT AGAGCAGGCC ATGGGCTTTG AGAACTTCAT CCAGGCCTAC 
AACAAGATCA AGGCTATTCA TGAGGATGAG GATGAAAACA TTGCAATGGG CTCCAGCATG 
GTGCAAAACA TTTTGGGAAC TGAGCACCAG CACTTGTATC CTAAAATCCT CCATTTGGTG 
ATGGCAGATG GAGCTTACCA GGAAGATAAT GATGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009292953.1
CDS	118..4356
Translation	MDKYERLKKIGEGSFGKAILVKSRTDGRQYVIKEIGISRMSNKERQESRKEVAVLANMSHPNIVQYKESFEESGCLYIVMDYCEGGDLFKKINNQRGSLFPEEQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIIRGSYPPVSIHYSPDLRSLLAQLFKRNPRERPSVSTILDKPFLARRIHKFLSPQLIAQEFSHSIHLQPKMSVAHAAPAKRPAPGPIPITSAQKITKPAAKYGVPLTVRRPSEVARKVPDAAKKPVKLKQAPPPAAVQRRVSRVEEERRKHEEGVRKKRMEMMERERKQREQMLLLKAAQMKRFEREKLNRINRAREQGWRHVLSSSGGSSPERKFYGGGGGNGAPHSPGISPFPVPGPSPVPSSALAPSRAPYDRIHPSAEKLTKPTPKDGAGEGAVHAGDNGRTSSDSGTLNGHACIPVKNGVRKEQRKTSLQNEQISKIDYANRFRGQLAVEKSKQVEEFLQRKREAMLNKVRAEGQLGARQNLAAIFASWTNSSRCRRPRVNKEEEEYLTRLRQIRLQNFNERQQIKARLRGEKYDSDGSDSQESCEEAVLRMKKIEALKAQSKARAAVLKEQLEKKRREAYEREKKAWEDHLVARGVKVAVGPVGGTPSPAAPPTPPSDPTPQEAPSKATPSSRPSTPAISMTAALKDVGAISSGQSSPMKEDSLKVESSDVQSDKKEILRRLNQKVQTTEEVSVKEPTPSSNQPCPDPQPVQPSITEERPSTAADRKKWEAVAPPILSVAEQTLEETCATLAVSQVASPETNTPSCGDRKKWQTDGVPLLSVAQQTLEETSFRTAGQLTEKTVGEVICMDAWQGEGLRKAWGQSPDSEVLKVLEEAELQSFTQRLDPASTCDQTLTESLNPIEESDATNEAKQVPPPEPCQPATDHDKQEVTCQETVPIIEEVKSQELIQTTAEGTEKSHSAESDIEVVDDMESVVLDDVPQKTAEPPPAEVIQAWTKDTPESLPAEGRVIQAECTEVPQREEKHPEPPADESLFVKLSSSPAHRRTVALAMLSAQSSLEDSSSAASRSRSVSPLRSHRHEDALLIGLSTGLFDANNPKMLRTCSLPDLSKVFSDATGPEGAAAPDSNLEIEDLEDKADEQSEPEDVYEDDDLRELRASMERLLQEQHSEDDDDEEEDEGGGSNGSPADEEPAGLNGLASAGDDKRSPEEETFNGMAEDDEDVSHGEEEPGGPVTNGMEFEDDEEQNSSEAQLNEEWQSDGSEEEDDEELEQQDSIFSRLEELRLHLEQAMGFENFIQAYNKIKAIHEDEDENIAMGSSMVQNILGTEHQHLYPKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_009294678.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio NIMA-related kinase 1 (nek1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009294678.2

ORF Insert Sequence:

ATGGATAAAT ATGAGAGGTT AAAGAAGATT GGGGAGGGAT CCTTTGGGAA AGCCATCTTG 
GTCAAGTCAA GGACAGATGG CCGACAATAT GTTATCAAGG AGATTGGTAT CTCCAGGATG 
TCGAACAAGG AACGACAGGA ATCACGTAAA GAGGTGGCAG TTCTGGCTAA CATGAGCCAT 
CCAAACATTG TCCAATACAA GGAATCCTTT GAAGAGAGTG GTTGTTTGTA CATTGTAATG 
GACTACTGTG AGGGAGGAGA CCTGTTTAAG AAGATCAATA ATCAAAGAGG ATCTCTTTTC 
CCAGAGGAAC AGATTCTTGA CTGGTTTGTG CAAATCTGTC TGGCTCTTAA ACATGTACAC 
GACCGCAAAA TCCTACATAG GGACATCAAA TCACAGAATA TTTTTCTGAC CAAAGATGGT 
ACTGTGCAGC TTGGAGACTT TGGAATCGCT AGAGTTTTGA ACAGCACCGT GGAGTTGGCA 
AGGACATGTA TCGGCACTCC TTATTACCTG TCGCCAGAGA TATGTGAAAA TAAACCATAC 
AACAACAAGA GTGATATCTG GGCCCTGGGA TGTGTCCTGT ATGAAATGTG CACGCTTAAG 
CATGCATTTG AAGCAGGAAA CATGAAGAAC CTGGTGCTGA AAATCATCCG TGGTTCATAC 
CCGCCTGTTT CCATCCATTA CTCCCCAGAT TTGCGCTCTC TCCTGGCCCA GCTCTTCAAG 
CGTAACCCCC GGGAGAGACC CTCCGTCTCG ACCATTTTGG ACAAGCCTTT TCTTGCTCGC 
AGAATTCACA AGTTTCTCAG CCCTCAGCTT ATTGCTCAGG AATTCAGCCA TTCAATTCAC 
CTTCAGCCCA AAATGAGTGT GGCCCATGCA GCACCAGCCA AACGTCCAGC CCCAGGCCCT 
ATCCCAATCA CCTCAGCTCA GAAAATCACC AAACCAGCAG CCAAATACGG AGTGCCTTTA 
ACAGTGCGGA GACCATCTGA AGTCGCCAGG AAGGTCCCAG ATGCAGCAAA GAAACCAGTT 
AAACTTAAGC AGGCTCCCCC TCCTGCAGCG GTTCAGAGGA GAGTGAGTCG AGTGGAGGAG 
GAGAGGAGAA AGCATGAGGA AGGAGTGAGG AAGAAGCGGA TGGAGATGAT GGAAAGAGAG 
AGAAAACAAA GAGAACAGAT GTTGTTACTG AAGGCTGCTC AGATGAAGAG ATTTGAGAGA 
GAAAAGCTGA ACCGAATCAA CCGAGCTCGA GAGCAGGGCT GGAGACACGT GCTGAGTTCT 
AGTGGAGGAA GCAGTCCTGA GCGGAAGTTT TATGGAGGTG GCGGCGGCAA TGGAGCTCCC 
CATTCCCCAG GAATATCTCC CTTTCCTGTG CCTGGACCCT CCCCTGTTCC TTCTTCAGCT 
TTAGCTCCAA GCAGAGCGCC ATATGACCGC ATCCACCCTT CAGCTGAAAA ATTAACCAAA 
CCTACACCTA AAGATGGTGC TGGTGAAGGA GCCGTGCATG CAGGGGACAA TGGCAGGACT 
TCTTCAGACT CAGGGACTCT AAATGGTCAT GCCTGCATTC CTGTTAAGAA TGGCGTCAGG 
AAAGAACAAC GTAAAACAAG TCTCCAAAAT GAGCAGATTT CAAAGATCGA CTATGCCAAT 
AGGTTTCGAG GTCAGCTGGC AGTAGAGAAA TCCAAGCAGG TGGAGGAGTT TCTACAGCGG 
AAAAGAGAAG CCATGCTGAA TAAAGTTCGT GCAGAAGGAC AGCTGGGGGC CCGGCAAAAC 
CTGGCCGCCA TCTTTGCAAG CTGGACAAAC TCCTCCCGAT GCAGGAGACC CCGAGTGAAC 
AAAGAGGAGG AGGAGTATTT GACCAGATTA CGACAGATCA GACTACAGAA CTTTAATGAA 
CGTCAGCAGA TTAAAGCTCG GCTCAGAGGG GAGAAGTATG ACAGTGATGG CTCAGACAGT 
CAGGAGTCAT GTGAAGAGGC AGTGCTCAGG ATGAAGAAGA TTGAGGCTTT GAAGGCCCAG 
TCAAAGGCTC GTGCTGCTGT ACTGAAAGAG CAGCTGGAAA AGAAGAGAAG AGAAGCGTAT 
GAAAGAGAGA AGAAAGCATG GGAGGATCAT CTTGTCGCTC GAGGAGTAAA AGTTGCAGTT 
GGGCCTGTGG GTGGGACTCC CTCTCCTGCA GCCCCTCCAA CTCCACCATC TGACCCCACC 
CCTCAGGAAG CTCCTTCTAA AGCCACACCC TCCTCTAGAC CCAGCACTCC AGCCATCTCT 
ATGACTGCTG CACTGAAGGA TGTGGGCGCG ATTTCATCAG GGCAGAGTTC TCCTATGAAA 
GAGGACTCCC TTAAAGTTGA ATCATCAGAT GTACAGAGTG ACAAGAAGGA GATTCTTCGC 
AGACTTAATC AGAAGGTTCA GACCACTGAG GAAGTTTCTG TAAAAGAGCC CACACCCTCC 
AGCAATCAAC CATGTCCTGA TCCTCAGCCA GTCCAACCCT CCATCACAGA GGAAAGGCCT 
TCCACAGCTG CAGATAGGAA GAAGTGGGAA GCCGTAGCTC CACCCATACT GTCTGTTGCT 
GAACAGACTC TCGAGGAAAC CTGTGCTACT CTGGCAGTGT CTCAGGTGGC ATCACCAGAA 
ACGAATACTC CCTCGTGTGG AGACAGGAAG AAATGGCAGA CAGATGGTGT TCCTCTTCTG 
TCTGTGGCTC AGCAGACACT GGAAGAAACC AGCTTTAGAA CAGCTGGTCA ATTAACAGAA 
AAAACAGTAG GTGAAGTCAT TTGCATGGAT GCCTGGCAGG GGGAGGGGCT AAGGAAGGCT 
TGGGGGCAGA GTCCAGATTC AGAGGTTTTG AAGGTGTTGG AAGAGGCAGA ACTTCAGTCA 
TTTACTCAGA GACTAGATCC TGCCAGCACG TGTGACCAAA CACTGACTGA AAGCCTTAAT 
CCCATTGAGG AATCGGATGC CACTAATGAA GCCAAGCAAG TGCCTCCTCC TGAACCGTGC 
CAGCCTGCCA CTGACCATGA CAAACAAGAA GTGACATGTC AAGAGACAGT GCCAATCATA 
GAAGAGGTTA AAAGTCAGGA ACTTATCCAA ACAACAGCAG AGGGGACAGA AAAATCTCAT 
TCTGCAGAAA GTGACATAGA GGTGGTGGAT GATATGGAGT CAGTTGTTCT GGATGATGTA 
CCACAGAAAA CCGCAGAGCC GCCCCCTGCA GAAGTAATTC AGGCGTGGAC TAAAGACACT 
CCAGAGTCTC TGCCAGCAGA GGGCAGAGTA ATACAAGCAG AATGCACAGA GGTACCTCAG 
AGAGAAGAGA AACATCCTGA ACCTCCAGCA GATGAGTCTT TGTTTGTGAA GTTAAGCAGT 
AGTCCAGCTC ACAGACGCAC AGTAGCGTTG GCCATGCTGT CTGCTCAATC CTCATTGGAA 
GACTCTTCCT CTGCTGCCTC ACGTTCTCGA TCAGTGTCTC CACTGCGCTC CCACCGCCAT 
GAAGATGCAC TGCTCATCGG CCTCTCCACT GGCCTCTTCG ATGCAAATAA CCCAAAGATG 
CTAAGGACGT GCTCCTTACC AGACCTCAGT AAGGTGTTTA GTGATGCTAC AGGACCTGAA 
GGTGCAGCCG CTCCTGACAG CAACCTAGAA ATTGAAGATC TGGAAGATAA AGCAGATGAG 
CAGTCTGAAC CTGAGGATGT GTATGAGGAT GACGATTTAA GAGAGCTCAG AGCATCTATG 
GAGAGGCTTC TTCAGGAGCA GCACAGTGAA GATGATGATG ATGAAGAAGA GGATGAGGGT 
GGTGGATCTA ATGGCAGCCC GGCCGATGAA GAGCCAGCTG GTCTTAATGG GTTAGCTAGT 
GCTGGTGATG ACAAACGCAG CCCAGAGGAA GAGACTTTTA ATGGAATGGC TGAAGATGAT 
GAGGATGTTA GCCATGGTGA AGAAGAACCA GGAGGCCCAG TCACAAATGG AATGGAATTT 
GAAGATGATG AAGAGCAGAA CAGCAGTGAA GCACAGCTCA ATGAGGAGTG GCAGTCAGAT 
GGCAGCGAGG AGGAGGACGA CGAAGAGCTG GAACAGCAGG ACAGCATCTT CAGTCGTCTG 
GAGGAGCTGC GGCTTCATCT AGAGCAGGCC ATGGGCTTTG AGAACTTCAT CCAGGCCTAC 
AACAAGATCA AGGCTATTCA TGAGGATGAG GATGAAAACA TTGCAATGGG CTCCAGCATG 
GTGCAAAACA TTTTGGGAAC TGAGCACCAG CACTTGTATC CTAAAATCCT CCATTTGGTG 
ATGGCAGATG GAGCTTACCA GGAAGATAAT GATGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_009294679.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009292954.1 Danio rerio NIMA-related kinase 1 (nek1), transcript variant X3, mRNA.

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CloneID	ODa42284
Clone ID Related Accession (Same CDS sequence)	XM_009294679.3 , XM_009294679.2
Accession Version	XM_009294679.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4239bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase Nek1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009294679.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAAAT ATGAGAGGTT AAAGAAGATT GGGGAGGGAT CCTTTGGGAA AGCCATCTTG 
GTCAAGTCAA GGACAGATGG CCGACAATAT GTTATCAAGG AGATTGGTAT CTCCAGGATG 
TCGAACAAGG AACGACAGGA ATCACGTAAA GAGGTGGCAG TTCTGGCTAA CATGAGCCAT 
CCAAACATTG TCCAATACAA GGAATCCTTT GAAGAGAGTG GTTGTTTGTA CATTGTAATG 
GACTACTGTG AGGGAGGAGA CCTGTTTAAG AAGATCAATA ATCAAAGAGG ATCTCTTTTC 
CCAGAGGAAC AGATTCTTGA CTGGTTTGTG CAAATCTGTC TGGCTCTTAA ACATGTACAC 
GACCGCAAAA TCCTACATAG GGACATCAAA TCACAGAATA TTTTTCTGAC CAAAGATGGT 
ACTGTGCAGC TTGGAGACTT TGGAATCGCT AGAGTTTTGA ACAGCACCGT GGAGTTGGCA 
AGGACATGTA TCGGCACTCC TTATTACCTG TCGCCAGAGA TATGTGAAAA TAAACCATAC 
AACAACAAGA GTGATATCTG GGCCCTGGGA TGTGTCCTGT ATGAAATGTG CACGCTTAAG 
CATGCATTTG AAGCAGGAAA CATGAAGAAC CTGGTGCTGA AAATCATCCG TGGTTCATAC 
CCGCCTGTTT CCATCCATTA CTCCCCAGAT TTGCGCTCTC TCCTGGCCCA GCTCTTCAAG 
CGTAACCCCC GGGAGAGACC CTCCGTCTCG ACCATTTTGG ACAAGCCTTT TCTTGCTCGC 
AGAATTCACA AGTTTCTCAG CCCTCAGCTT ATTGCTCAGG AATTCAGCCA TTCAATTCAC 
CTTCAGCCCA AAATGAGTGT GGCCCATGCA GCACCAGCCA AACGTCCAGC CCCAGGCCCT 
ATCCCAATCA CCTCAGCTCA GAAAATCACC AAACCAGCAG CCAAATACGG AGTGCCTTTA 
ACAGTGCGGA GACCATCTGA AGTCGCCAGG AAGGTCCCAG ATGCAGCAAA GAAACCAGTT 
AAACTTAAGC AGGCTCCCCC TCCTGCAGCG GTTCAGAGGA GAGTGAGTCG AGTGGAGGAG 
GAGAGGAGAA AGCATGAGGA AGGAGTGAGG AAGAAGCGGA TGGAGATGAT GGAAAGAGAG 
AGAAAACAAA GAGAACAGAT GTTGTTACTG AAGGCTGCTC AGATGAAGAG ATTTGAGAGA 
GAAAAGCTGA ACCGAATCAA CCGAGCTCGA GAGCAGGGCT GGAGACACGT GCTGAGTTCT 
AGTGGAGGAA GCAGTCCTGA GCGGAAGTTT TATGGAGGTG GCGGCGGCAA TGGAGCTCCC 
CATTCCCCAG GAATATCTCC CTTTCCTGTG CCTGGACCCT CCCCTGTTCC TTCTTCAGCT 
TTAGCTCCAA GCAGAGCGCC ATATGACCGC ATCCACCCTT CAGCTGAAAA ATTAACCAAA 
CCTACACCTA AAGATGGTGC TGGTGAAGGA GCCGTGCATG CAGGGGACAA TGGCAGGACT 
TCTTCAGACT CAGGGACTCT AAATGGTCAT GCCTGCATTC CTGTTAAGAA TGGCGTCAGG 
AAAGAACAAC GTAAAACAAG TCTCCAAAAT GAGCAGATTT CAAAGATCGA CTATGCCAAT 
AGGTTTCGAG GTCAGCTGGC AGTAGAGAAA TCCAAGCAGG TGGAGGAGTT TCTACAGCGG 
AAAAGAGAAG CCATGCTGAA TAAAGTTCGT GCAGAAGGAC AGCTGGGGGC CCGGCAAAAC 
CTGGCCGCCA TCTTTGCAAG CTGGACAAAC TCCTCCCGAT GCAGGAGACC CCGAGTGAAC 
AAAGAGGAGG AGGAGTATTT GACCAGATTA CGACAGATCA GACTACAGAA CTTTAATGAA 
CGTCAGCAGA TTAAAGCTCG GCTCAGAGGG GAGAAGTATG ACAGTGATGG CTCAGACAGT 
CAGGAGTCAT GTGAAGAGGC AGTGCTCAGG ATGAAGAAGA TTGAGGCTTT GAAGGCCCAG 
TCAAAGGCTC GTGCTGCTGT ACTGAAAGAG CAGCTGGAAA AGAAGAGAAG AGAAGCGTAT 
GAAAGAGAGA AGAAAGCATG GGAGGATCAT CAGCTTGTCG CTCGAGGAGT AAAAGTTGCA 
GTTGGGCCTG TGGGTGGGAC TCCCTCTCCT GCAGCCCCTC CAACTCCACC ATCTGACCCC 
ACCCCTCAGG AAGCTCCTTC TAAAGCCACA CCCTCCTCTA GACCCAGCAC TCCAGCCATC 
TCTATGACTG CTGCACTGAA GGATGTGGGC GCGATTTCAT CAGGGCAGAG TTCTCCTATG 
AAAGAGGACT CCCTTAAAGT TGAATCATCA GATGTACAGA GTGACAAGAA GGAGATTCTT 
CGCAGACTTA ATCAGAAGGT TCAGACCACT GAGGAAGTTT CTGTAAAAGA GCCCACACCC 
TCCAGCAATC AACCATGTCC TGATCCTCAG CCAGTCCAAC CCTCCATCAC AGAGGAAAGG 
CCTTCCACAG CTGCAGATAG GAAGAAGTGG GAAGCCGTAG CTCCACCCAT ACTGTCTGTT 
GCTGAACAGA CTCTCGAGGA AACCTGTGCT ACTCTGGCAG TGTCTCAGGT GGCATCACCA 
GAAACGAATA CTCCCTCGTG TGGAGACAGG AAGAAATGGC AGACAGATGG TGTTCCTCTT 
CTGTCTGTGG CTCAGCAGAC ACTGGAAGAA ACCAGCTTTA GAACAGCTGG TCAATTAACA 
GAAAAAACAG TAGGTGAAGT CATTTGCATG GATGCCTGGC AGGGGGAGGG GCTAAGGAAG 
GCTTGGGGGC AGAGTCCAGA TTCAGAGGTT TTGAAGGTGT TGGAAGAGGC AGAACTTCAG 
TCATTTACTC AGAGACTAGA TCCTGCCAGC ACGTGTGACC AAACACTGAC TGAAAGCCTT 
AATCCCATTG AGGAATCGGA TGCCACTAAT GAAGCCAAGC AAGTGCCTCC TCCTGAACCG 
TGCCAGCCTG CCACTGACCA TGACAAACAA GAAGTGACAT GTCAAGAGAC AGTGCCAATC 
ATAGAAGAGG TTAAAAGTCA GGAACTTATC CAAACAACAG CAGAGGGGAC AGAAAAATCT 
CATTCTGCAG AAAGTGACAT AGAGGTGGTG GATGATATGG AGTCAGTTGT TCTGGATGAT 
GTACCACAGA AAACCGCAGA GCCGCCCCCT GCAGAAGTAA TTCAGGCGTG GACTAAAGAC 
ACTCCAGAGT CTCTGCCAGC AGAGGGCAGA GTAATACAAG CAGAATGCAC AGAGGTACCT 
CAGAGAGAAG AGAAACATCC TGAACCTCCA GATGAGTCTT TGTTTGTGAA GTTAAGCAGT 
AGTCCAGCTC ACAGACGCAC AGTAGCGTTG GCCATGCTGT CTGCTCAATC CTCATTGGAA 
GACTCTTCCT CTGCTGCCTC ACGTTCTCGA TCAGTGTCTC CACTGCGCTC CCACCGCCAT 
GAAGATGCAC TGCTCATCGG CCTCTCCACT GGCCTCTTCG ATGCAAATAA CCCAAAGATG 
CTAAGGACGT GCTCCTTACC AGACCTCAGT AAGGTGTTTA GTGATGCTAC AGGACCTGAA 
GGTGCAGCCG CTCCTGACAG CAACCTAGAA ATTGAAGATC TGGAAGATAA AGCAGATGAG 
CAGTCTGAAC CTGAGGATGT GTATGAGGAT GACGATTTAA GAGAGCTCAG AGCATCTATG 
GAGAGGCTTC TTCAGGAGCA GCACAGTGAA GATGATGATG ATGAAGAAGA GGATGAGGGT 
GGTGGATCTA ATGGCAGCCC GGCCGATGAA GAGCCAGCTG GTCTTAATGG GTTAGCTAGT 
GCTGGTGATG ACAAACGCAG CCCAGAGGAA GAGACTTTTA ATGGAATGGC TGAAGATGAT 
GAGGATGTTA GCCATGGTGA AGAAGAACCA GGAGGCCCAG TCACAAATGG AATGGAATTT 
GAAGATGATG AAGAGCAGAA CAGCAGTGAA GCACAGCTCA ATGAGGAGTG GCAGTCAGAT 
GGCAGCGAGG AGGAGGACGA CGAAGAGCTG GAACAGCAGG ACAGCATCTT CAGTCGTCTG 
GAGGAGCTGC GGCTTCATCT AGAGCAGGCC ATGGGCTTTG AGAACTTCAT CCAGGCCTAC 
AACAAGATCA AGGCTATTCA TGAGGATGAG GATGAAAACA TTGCAATGGG CTCCAGCATG 
GTGCAAAACA TTTTGGGAAC TGAGCACCAG CACTTGTATC CTAAAATCCT CCATTTGGTG 
ATGGCAGATG GAGCTTACCA GGAAGATAAT GATGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009292954.1
CDS	127..4365
Translation	MDKYERLKKIGEGSFGKAILVKSRTDGRQYVIKEIGISRMSNKERQESRKEVAVLANMSHPNIVQYKESFEESGCLYIVMDYCEGGDLFKKINNQRGSLFPEEQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIIRGSYPPVSIHYSPDLRSLLAQLFKRNPRERPSVSTILDKPFLARRIHKFLSPQLIAQEFSHSIHLQPKMSVAHAAPAKRPAPGPIPITSAQKITKPAAKYGVPLTVRRPSEVARKVPDAAKKPVKLKQAPPPAAVQRRVSRVEEERRKHEEGVRKKRMEMMERERKQREQMLLLKAAQMKRFEREKLNRINRAREQGWRHVLSSSGGSSPERKFYGGGGGNGAPHSPGISPFPVPGPSPVPSSALAPSRAPYDRIHPSAEKLTKPTPKDGAGEGAVHAGDNGRTSSDSGTLNGHACIPVKNGVRKEQRKTSLQNEQISKIDYANRFRGQLAVEKSKQVEEFLQRKREAMLNKVRAEGQLGARQNLAAIFASWTNSSRCRRPRVNKEEEEYLTRLRQIRLQNFNERQQIKARLRGEKYDSDGSDSQESCEEAVLRMKKIEALKAQSKARAAVLKEQLEKKRREAYEREKKAWEDHQLVARGVKVAVGPVGGTPSPAAPPTPPSDPTPQEAPSKATPSSRPSTPAISMTAALKDVGAISSGQSSPMKEDSLKVESSDVQSDKKEILRRLNQKVQTTEEVSVKEPTPSSNQPCPDPQPVQPSITEERPSTAADRKKWEAVAPPILSVAEQTLEETCATLAVSQVASPETNTPSCGDRKKWQTDGVPLLSVAQQTLEETSFRTAGQLTEKTVGEVICMDAWQGEGLRKAWGQSPDSEVLKVLEEAELQSFTQRLDPASTCDQTLTESLNPIEESDATNEAKQVPPPEPCQPATDHDKQEVTCQETVPIIEEVKSQELIQTTAEGTEKSHSAESDIEVVDDMESVVLDDVPQKTAEPPPAEVIQAWTKDTPESLPAEGRVIQAECTEVPQREEKHPEPPDESLFVKLSSSPAHRRTVALAMLSAQSSLEDSSSAASRSRSVSPLRSHRHEDALLIGLSTGLFDANNPKMLRTCSLPDLSKVFSDATGPEGAAAPDSNLEIEDLEDKADEQSEPEDVYEDDDLRELRASMERLLQEQHSEDDDDEEEDEGGGSNGSPADEEPAGLNGLASAGDDKRSPEEETFNGMAEDDEDVSHGEEEPGGPVTNGMEFEDDEEQNSSEAQLNEEWQSDGSEEEDDEELEQQDSIFSRLEELRLHLEQAMGFENFIQAYNKIKAIHEDEDENIAMGSSMVQNILGTEHQHLYPKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_009294679.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio NIMA-related kinase 1 (nek1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009294679.3

ORF Insert Sequence:

ATGGATAAAT ATGAGAGGTT AAAGAAGATT GGGGAGGGAT CCTTTGGGAA AGCCATCTTG 
GTCAAGTCAA GGACAGATGG CCGACAATAT GTTATCAAGG AGATTGGTAT CTCCAGGATG 
TCGAACAAGG AACGACAGGA ATCACGTAAA GAGGTGGCAG TTCTGGCTAA CATGAGCCAT 
CCAAACATTG TCCAATACAA GGAATCCTTT GAAGAGAGTG GTTGTTTGTA CATTGTAATG 
GACTACTGTG AGGGAGGAGA CCTGTTTAAG AAGATCAATA ATCAAAGAGG ATCTCTTTTC 
CCAGAGGAAC AGATTCTTGA CTGGTTTGTG CAAATCTGTC TGGCTCTTAA ACATGTACAC 
GACCGCAAAA TCCTACATAG GGACATCAAA TCACAGAATA TTTTTCTGAC CAAAGATGGT 
ACTGTGCAGC TTGGAGACTT TGGAATCGCT AGAGTTTTGA ACAGCACCGT GGAGTTGGCA 
AGGACATGTA TCGGCACTCC TTATTACCTG TCGCCAGAGA TATGTGAAAA TAAACCATAC 
AACAACAAGA GTGATATCTG GGCCCTGGGA TGTGTCCTGT ATGAAATGTG CACGCTTAAG 
CATGCATTTG AAGCAGGAAA CATGAAGAAC CTGGTGCTGA AAATCATCCG TGGTTCATAC 
CCGCCTGTTT CCATCCATTA CTCCCCAGAT TTGCGCTCTC TCCTGGCCCA GCTCTTCAAG 
CGTAACCCCC GGGAGAGACC CTCCGTCTCG ACCATTTTGG ACAAGCCTTT TCTTGCTCGC 
AGAATTCACA AGTTTCTCAG CCCTCAGCTT ATTGCTCAGG AATTCAGCCA TTCAATTCAC 
CTTCAGCCCA AAATGAGTGT GGCCCATGCA GCACCAGCCA AACGTCCAGC CCCAGGCCCT 
ATCCCAATCA CCTCAGCTCA GAAAATCACC AAACCAGCAG CCAAATACGG AGTGCCTTTA 
ACAGTGCGGA GACCATCTGA AGTCGCCAGG AAGGTCCCAG ATGCAGCAAA GAAACCAGTT 
AAACTTAAGC AGGCTCCCCC TCCTGCAGCG GTTCAGAGGA GAGTGAGTCG AGTGGAGGAG 
GAGAGGAGAA AGCATGAGGA AGGAGTGAGG AAGAAGCGGA TGGAGATGAT GGAAAGAGAG 
AGAAAACAAA GAGAACAGAT GTTGTTACTG AAGGCTGCTC AGATGAAGAG ATTTGAGAGA 
GAAAAGCTGA ACCGAATCAA CCGAGCTCGA GAGCAGGGCT GGAGACACGT GCTGAGTTCT 
AGTGGAGGAA GCAGTCCTGA GCGGAAGTTT TATGGAGGTG GCGGCGGCAA TGGAGCTCCC 
CATTCCCCAG GAATATCTCC CTTTCCTGTG CCTGGACCCT CCCCTGTTCC TTCTTCAGCT 
TTAGCTCCAA GCAGAGCGCC ATATGACCGC ATCCACCCTT CAGCTGAAAA ATTAACCAAA 
CCTACACCTA AAGATGGTGC TGGTGAAGGA GCCGTGCATG CAGGGGACAA TGGCAGGACT 
TCTTCAGACT CAGGGACTCT AAATGGTCAT GCCTGCATTC CTGTTAAGAA TGGCGTCAGG 
AAAGAACAAC GTAAAACAAG TCTCCAAAAT GAGCAGATTT CAAAGATCGA CTATGCCAAT 
AGGTTTCGAG GTCAGCTGGC AGTAGAGAAA TCCAAGCAGG TGGAGGAGTT TCTACAGCGG 
AAAAGAGAAG CCATGCTGAA TAAAGTTCGT GCAGAAGGAC AGCTGGGGGC CCGGCAAAAC 
CTGGCCGCCA TCTTTGCAAG CTGGACAAAC TCCTCCCGAT GCAGGAGACC CCGAGTGAAC 
AAAGAGGAGG AGGAGTATTT GACCAGATTA CGACAGATCA GACTACAGAA CTTTAATGAA 
CGTCAGCAGA TTAAAGCTCG GCTCAGAGGG GAGAAGTATG ACAGTGATGG CTCAGACAGT 
CAGGAGTCAT GTGAAGAGGC AGTGCTCAGG ATGAAGAAGA TTGAGGCTTT GAAGGCCCAG 
TCAAAGGCTC GTGCTGCTGT ACTGAAAGAG CAGCTGGAAA AGAAGAGAAG AGAAGCGTAT 
GAAAGAGAGA AGAAAGCATG GGAGGATCAT CAGCTTGTCG CTCGAGGAGT AAAAGTTGCA 
GTTGGGCCTG TGGGTGGGAC TCCCTCTCCT GCAGCCCCTC CAACTCCACC ATCTGACCCC 
ACCCCTCAGG AAGCTCCTTC TAAAGCCACA CCCTCCTCTA GACCCAGCAC TCCAGCCATC 
TCTATGACTG CTGCACTGAA GGATGTGGGC GCGATTTCAT CAGGGCAGAG TTCTCCTATG 
AAAGAGGACT CCCTTAAAGT TGAATCATCA GATGTACAGA GTGACAAGAA GGAGATTCTT 
CGCAGACTTA ATCAGAAGGT TCAGACCACT GAGGAAGTTT CTGTAAAAGA GCCCACACCC 
TCCAGCAATC AACCATGTCC TGATCCTCAG CCAGTCCAAC CCTCCATCAC AGAGGAAAGG 
CCTTCCACAG CTGCAGATAG GAAGAAGTGG GAAGCCGTAG CTCCACCCAT ACTGTCTGTT 
GCTGAACAGA CTCTCGAGGA AACCTGTGCT ACTCTGGCAG TGTCTCAGGT GGCATCACCA 
GAAACGAATA CTCCCTCGTG TGGAGACAGG AAGAAATGGC AGACAGATGG TGTTCCTCTT 
CTGTCTGTGG CTCAGCAGAC ACTGGAAGAA ACCAGCTTTA GAACAGCTGG TCAATTAACA 
GAAAAAACAG TAGGTGAAGT CATTTGCATG GATGCCTGGC AGGGGGAGGG GCTAAGGAAG 
GCTTGGGGGC AGAGTCCAGA TTCAGAGGTT TTGAAGGTGT TGGAAGAGGC AGAACTTCAG 
TCATTTACTC AGAGACTAGA TCCTGCCAGC ACGTGTGACC AAACACTGAC TGAAAGCCTT 
AATCCCATTG AGGAATCGGA TGCCACTAAT GAAGCCAAGC AAGTGCCTCC TCCTGAACCG 
TGCCAGCCTG CCACTGACCA TGACAAACAA GAAGTGACAT GTCAAGAGAC AGTGCCAATC 
ATAGAAGAGG TTAAAAGTCA GGAACTTATC CAAACAACAG CAGAGGGGAC AGAAAAATCT 
CATTCTGCAG AAAGTGACAT AGAGGTGGTG GATGATATGG AGTCAGTTGT TCTGGATGAT 
GTACCACAGA AAACCGCAGA GCCGCCCCCT GCAGAAGTAA TTCAGGCGTG GACTAAAGAC 
ACTCCAGAGT CTCTGCCAGC AGAGGGCAGA GTAATACAAG CAGAATGCAC AGAGGTACCT 
CAGAGAGAAG AGAAACATCC TGAACCTCCA GATGAGTCTT TGTTTGTGAA GTTAAGCAGT 
AGTCCAGCTC ACAGACGCAC AGTAGCGTTG GCCATGCTGT CTGCTCAATC CTCATTGGAA 
GACTCTTCCT CTGCTGCCTC ACGTTCTCGA TCAGTGTCTC CACTGCGCTC CCACCGCCAT 
GAAGATGCAC TGCTCATCGG CCTCTCCACT GGCCTCTTCG ATGCAAATAA CCCAAAGATG 
CTAAGGACGT GCTCCTTACC AGACCTCAGT AAGGTGTTTA GTGATGCTAC AGGACCTGAA 
GGTGCAGCCG CTCCTGACAG CAACCTAGAA ATTGAAGATC TGGAAGATAA AGCAGATGAG 
CAGTCTGAAC CTGAGGATGT GTATGAGGAT GACGATTTAA GAGAGCTCAG AGCATCTATG 
GAGAGGCTTC TTCAGGAGCA GCACAGTGAA GATGATGATG ATGAAGAAGA GGATGAGGGT 
GGTGGATCTA ATGGCAGCCC GGCCGATGAA GAGCCAGCTG GTCTTAATGG GTTAGCTAGT 
GCTGGTGATG ACAAACGCAG CCCAGAGGAA GAGACTTTTA ATGGAATGGC TGAAGATGAT 
GAGGATGTTA GCCATGGTGA AGAAGAACCA GGAGGCCCAG TCACAAATGG AATGGAATTT 
GAAGATGATG AAGAGCAGAA CAGCAGTGAA GCACAGCTCA ATGAGGAGTG GCAGTCAGAT 
GGCAGCGAGG AGGAGGACGA CGAAGAGCTG GAACAGCAGG ACAGCATCTT CAGTCGTCTG 
GAGGAGCTGC GGCTTCATCT AGAGCAGGCC ATGGGCTTTG AGAACTTCAT CCAGGCCTAC 
AACAAGATCA AGGCTATTCA TGAGGATGAG GATGAAAACA TTGCAATGGG CTCCAGCATG 
GTGCAAAACA TTTTGGGAAC TGAGCACCAG CACTTGTATC CTAAAATCCT CCATTTGGTG 
ATGGCAGATG GAGCTTACCA GGAAGATAAT GATGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_009294679.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009292954.1 Danio rerio NIMA-related kinase 1 (nek1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa42284
Clone ID Related Accession (Same CDS sequence)	XM_009294679.3 , XM_009294679.2
Accession Version	XM_009294679.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4239bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	serine/threonine-protein kinase Nek1 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009294679.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAAAT ATGAGAGGTT AAAGAAGATT GGGGAGGGAT CCTTTGGGAA AGCCATCTTG 
GTCAAGTCAA GGACAGATGG CCGACAATAT GTTATCAAGG AGATTGGTAT CTCCAGGATG 
TCGAACAAGG AACGACAGGA ATCACGTAAA GAGGTGGCAG TTCTGGCTAA CATGAGCCAT 
CCAAACATTG TCCAATACAA GGAATCCTTT GAAGAGAGTG GTTGTTTGTA CATTGTAATG 
GACTACTGTG AGGGAGGAGA CCTGTTTAAG AAGATCAATA ATCAAAGAGG ATCTCTTTTC 
CCAGAGGAAC AGATTCTTGA CTGGTTTGTG CAAATCTGTC TGGCTCTTAA ACATGTACAC 
GACCGCAAAA TCCTACATAG GGACATCAAA TCACAGAATA TTTTTCTGAC CAAAGATGGT 
ACTGTGCAGC TTGGAGACTT TGGAATCGCT AGAGTTTTGA ACAGCACCGT GGAGTTGGCA 
AGGACATGTA TCGGCACTCC TTATTACCTG TCGCCAGAGA TATGTGAAAA TAAACCATAC 
AACAACAAGA GTGATATCTG GGCCCTGGGA TGTGTCCTGT ATGAAATGTG CACGCTTAAG 
CATGCATTTG AAGCAGGAAA CATGAAGAAC CTGGTGCTGA AAATCATCCG TGGTTCATAC 
CCGCCTGTTT CCATCCATTA CTCCCCAGAT TTGCGCTCTC TCCTGGCCCA GCTCTTCAAG 
CGTAACCCCC GGGAGAGACC CTCCGTCTCG ACCATTTTGG ACAAGCCTTT TCTTGCTCGC 
AGAATTCACA AGTTTCTCAG CCCTCAGCTT ATTGCTCAGG AATTCAGCCA TTCAATTCAC 
CTTCAGCCCA AAATGAGTGT GGCCCATGCA GCACCAGCCA AACGTCCAGC CCCAGGCCCT 
ATCCCAATCA CCTCAGCTCA GAAAATCACC AAACCAGCAG CCAAATACGG AGTGCCTTTA 
ACAGTGCGGA GACCATCTGA AGTCGCCAGG AAGGTCCCAG ATGCAGCAAA GAAACCAGTT 
AAACTTAAGC AGGCTCCCCC TCCTGCAGCG GTTCAGAGGA GAGTGAGTCG AGTGGAGGAG 
GAGAGGAGAA AGCATGAGGA AGGAGTGAGG AAGAAGCGGA TGGAGATGAT GGAAAGAGAG 
AGAAAACAAA GAGAACAGAT GTTGTTACTG AAGGCTGCTC AGATGAAGAG ATTTGAGAGA 
GAAAAGCTGA ACCGAATCAA CCGAGCTCGA GAGCAGGGCT GGAGACACGT GCTGAGTTCT 
AGTGGAGGAA GCAGTCCTGA GCGGAAGTTT TATGGAGGTG GCGGCGGCAA TGGAGCTCCC 
CATTCCCCAG GAATATCTCC CTTTCCTGTG CCTGGACCCT CCCCTGTTCC TTCTTCAGCT 
TTAGCTCCAA GCAGAGCGCC ATATGACCGC ATCCACCCTT CAGCTGAAAA ATTAACCAAA 
CCTACACCTA AAGATGGTGC TGGTGAAGGA GCCGTGCATG CAGGGGACAA TGGCAGGACT 
TCTTCAGACT CAGGGACTCT AAATGGTCAT GCCTGCATTC CTGTTAAGAA TGGCGTCAGG 
AAAGAACAAC GTAAAACAAG TCTCCAAAAT GAGCAGATTT CAAAGATCGA CTATGCCAAT 
AGGTTTCGAG GTCAGCTGGC AGTAGAGAAA TCCAAGCAGG TGGAGGAGTT TCTACAGCGG 
AAAAGAGAAG CCATGCTGAA TAAAGTTCGT GCAGAAGGAC AGCTGGGGGC CCGGCAAAAC 
CTGGCCGCCA TCTTTGCAAG CTGGACAAAC TCCTCCCGAT GCAGGAGACC CCGAGTGAAC 
AAAGAGGAGG AGGAGTATTT GACCAGATTA CGACAGATCA GACTACAGAA CTTTAATGAA 
CGTCAGCAGA TTAAAGCTCG GCTCAGAGGG GAGAAGTATG ACAGTGATGG CTCAGACAGT 
CAGGAGTCAT GTGAAGAGGC AGTGCTCAGG ATGAAGAAGA TTGAGGCTTT GAAGGCCCAG 
TCAAAGGCTC GTGCTGCTGT ACTGAAAGAG CAGCTGGAAA AGAAGAGAAG AGAAGCGTAT 
GAAAGAGAGA AGAAAGCATG GGAGGATCAT CAGCTTGTCG CTCGAGGAGT AAAAGTTGCA 
GTTGGGCCTG TGGGTGGGAC TCCCTCTCCT GCAGCCCCTC CAACTCCACC ATCTGACCCC 
ACCCCTCAGG AAGCTCCTTC TAAAGCCACA CCCTCCTCTA GACCCAGCAC TCCAGCCATC 
TCTATGACTG CTGCACTGAA GGATGTGGGC GCGATTTCAT CAGGGCAGAG TTCTCCTATG 
AAAGAGGACT CCCTTAAAGT TGAATCATCA GATGTACAGA GTGACAAGAA GGAGATTCTT 
CGCAGACTTA ATCAGAAGGT TCAGACCACT GAGGAAGTTT CTGTAAAAGA GCCCACACCC 
TCCAGCAATC AACCATGTCC TGATCCTCAG CCAGTCCAAC CCTCCATCAC AGAGGAAAGG 
CCTTCCACAG CTGCAGATAG GAAGAAGTGG GAAGCCGTAG CTCCACCCAT ACTGTCTGTT 
GCTGAACAGA CTCTCGAGGA AACCTGTGCT ACTCTGGCAG TGTCTCAGGT GGCATCACCA 
GAAACGAATA CTCCCTCGTG TGGAGACAGG AAGAAATGGC AGACAGATGG TGTTCCTCTT 
CTGTCTGTGG CTCAGCAGAC ACTGGAAGAA ACCAGCTTTA GAACAGCTGG TCAATTAACA 
GAAAAAACAG TAGGTGAAGT CATTTGCATG GATGCCTGGC AGGGGGAGGG GCTAAGGAAG 
GCTTGGGGGC AGAGTCCAGA TTCAGAGGTT TTGAAGGTGT TGGAAGAGGC AGAACTTCAG 
TCATTTACTC AGAGACTAGA TCCTGCCAGC ACGTGTGACC AAACACTGAC TGAAAGCCTT 
AATCCCATTG AGGAATCGGA TGCCACTAAT GAAGCCAAGC AAGTGCCTCC TCCTGAACCG 
TGCCAGCCTG CCACTGACCA TGACAAACAA GAAGTGACAT GTCAAGAGAC AGTGCCAATC 
ATAGAAGAGG TTAAAAGTCA GGAACTTATC CAAACAACAG CAGAGGGGAC AGAAAAATCT 
CATTCTGCAG AAAGTGACAT AGAGGTGGTG GATGATATGG AGTCAGTTGT TCTGGATGAT 
GTACCACAGA AAACCGCAGA GCCGCCCCCT GCAGAAGTAA TTCAGGCGTG GACTAAAGAC 
ACTCCAGAGT CTCTGCCAGC AGAGGGCAGA GTAATACAAG CAGAATGCAC AGAGGTACCT 
CAGAGAGAAG AGAAACATCC TGAACCTCCA GATGAGTCTT TGTTTGTGAA GTTAAGCAGT 
AGTCCAGCTC ACAGACGCAC AGTAGCGTTG GCCATGCTGT CTGCTCAATC CTCATTGGAA 
GACTCTTCCT CTGCTGCCTC ACGTTCTCGA TCAGTGTCTC CACTGCGCTC CCACCGCCAT 
GAAGATGCAC TGCTCATCGG CCTCTCCACT GGCCTCTTCG ATGCAAATAA CCCAAAGATG 
CTAAGGACGT GCTCCTTACC AGACCTCAGT AAGGTGTTTA GTGATGCTAC AGGACCTGAA 
GGTGCAGCCG CTCCTGACAG CAACCTAGAA ATTGAAGATC TGGAAGATAA AGCAGATGAG 
CAGTCTGAAC CTGAGGATGT GTATGAGGAT GACGATTTAA GAGAGCTCAG AGCATCTATG 
GAGAGGCTTC TTCAGGAGCA GCACAGTGAA GATGATGATG ATGAAGAAGA GGATGAGGGT 
GGTGGATCTA ATGGCAGCCC GGCCGATGAA GAGCCAGCTG GTCTTAATGG GTTAGCTAGT 
GCTGGTGATG ACAAACGCAG CCCAGAGGAA GAGACTTTTA ATGGAATGGC TGAAGATGAT 
GAGGATGTTA GCCATGGTGA AGAAGAACCA GGAGGCCCAG TCACAAATGG AATGGAATTT 
GAAGATGATG AAGAGCAGAA CAGCAGTGAA GCACAGCTCA ATGAGGAGTG GCAGTCAGAT 
GGCAGCGAGG AGGAGGACGA CGAAGAGCTG GAACAGCAGG ACAGCATCTT CAGTCGTCTG 
GAGGAGCTGC GGCTTCATCT AGAGCAGGCC ATGGGCTTTG AGAACTTCAT CCAGGCCTAC 
AACAAGATCA AGGCTATTCA TGAGGATGAG GATGAAAACA TTGCAATGGG CTCCAGCATG 
GTGCAAAACA TTTTGGGAAC TGAGCACCAG CACTTGTATC CTAAAATCCT CCATTTGGTG 
ATGGCAGATG GAGCTTACCA GGAAGATAAT GATGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009292954.1
CDS	118..4356
Translation	MDKYERLKKIGEGSFGKAILVKSRTDGRQYVIKEIGISRMSNKERQESRKEVAVLANMSHPNIVQYKESFEESGCLYIVMDYCEGGDLFKKINNQRGSLFPEEQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIIRGSYPPVSIHYSPDLRSLLAQLFKRNPRERPSVSTILDKPFLARRIHKFLSPQLIAQEFSHSIHLQPKMSVAHAAPAKRPAPGPIPITSAQKITKPAAKYGVPLTVRRPSEVARKVPDAAKKPVKLKQAPPPAAVQRRVSRVEEERRKHEEGVRKKRMEMMERERKQREQMLLLKAAQMKRFEREKLNRINRAREQGWRHVLSSSGGSSPERKFYGGGGGNGAPHSPGISPFPVPGPSPVPSSALAPSRAPYDRIHPSAEKLTKPTPKDGAGEGAVHAGDNGRTSSDSGTLNGHACIPVKNGVRKEQRKTSLQNEQISKIDYANRFRGQLAVEKSKQVEEFLQRKREAMLNKVRAEGQLGARQNLAAIFASWTNSSRCRRPRVNKEEEEYLTRLRQIRLQNFNERQQIKARLRGEKYDSDGSDSQESCEEAVLRMKKIEALKAQSKARAAVLKEQLEKKRREAYEREKKAWEDHQLVARGVKVAVGPVGGTPSPAAPPTPPSDPTPQEAPSKATPSSRPSTPAISMTAALKDVGAISSGQSSPMKEDSLKVESSDVQSDKKEILRRLNQKVQTTEEVSVKEPTPSSNQPCPDPQPVQPSITEERPSTAADRKKWEAVAPPILSVAEQTLEETCATLAVSQVASPETNTPSCGDRKKWQTDGVPLLSVAQQTLEETSFRTAGQLTEKTVGEVICMDAWQGEGLRKAWGQSPDSEVLKVLEEAELQSFTQRLDPASTCDQTLTESLNPIEESDATNEAKQVPPPEPCQPATDHDKQEVTCQETVPIIEEVKSQELIQTTAEGTEKSHSAESDIEVVDDMESVVLDDVPQKTAEPPPAEVIQAWTKDTPESLPAEGRVIQAECTEVPQREEKHPEPPDESLFVKLSSSPAHRRTVALAMLSAQSSLEDSSSAASRSRSVSPLRSHRHEDALLIGLSTGLFDANNPKMLRTCSLPDLSKVFSDATGPEGAAAPDSNLEIEDLEDKADEQSEPEDVYEDDDLRELRASMERLLQEQHSEDDDDEEEDEGGGSNGSPADEEPAGLNGLASAGDDKRSPEEETFNGMAEDDEDVSHGEEEPGGPVTNGMEFEDDEEQNSSEAQLNEEWQSDGSEEEDDEELEQQDSIFSRLEELRLHLEQAMGFENFIQAYNKIKAIHEDEDENIAMGSSMVQNILGTEHQHLYPKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_009294679.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio NIMA-related kinase 1 (nek1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009294679.2

ORF Insert Sequence:

ATGGATAAAT ATGAGAGGTT AAAGAAGATT GGGGAGGGAT CCTTTGGGAA AGCCATCTTG 
GTCAAGTCAA GGACAGATGG CCGACAATAT GTTATCAAGG AGATTGGTAT CTCCAGGATG 
TCGAACAAGG AACGACAGGA ATCACGTAAA GAGGTGGCAG TTCTGGCTAA CATGAGCCAT 
CCAAACATTG TCCAATACAA GGAATCCTTT GAAGAGAGTG GTTGTTTGTA CATTGTAATG 
GACTACTGTG AGGGAGGAGA CCTGTTTAAG AAGATCAATA ATCAAAGAGG ATCTCTTTTC 
CCAGAGGAAC AGATTCTTGA CTGGTTTGTG CAAATCTGTC TGGCTCTTAA ACATGTACAC 
GACCGCAAAA TCCTACATAG GGACATCAAA TCACAGAATA TTTTTCTGAC CAAAGATGGT 
ACTGTGCAGC TTGGAGACTT TGGAATCGCT AGAGTTTTGA ACAGCACCGT GGAGTTGGCA 
AGGACATGTA TCGGCACTCC TTATTACCTG TCGCCAGAGA TATGTGAAAA TAAACCATAC 
AACAACAAGA GTGATATCTG GGCCCTGGGA TGTGTCCTGT ATGAAATGTG CACGCTTAAG 
CATGCATTTG AAGCAGGAAA CATGAAGAAC CTGGTGCTGA AAATCATCCG TGGTTCATAC 
CCGCCTGTTT CCATCCATTA CTCCCCAGAT TTGCGCTCTC TCCTGGCCCA GCTCTTCAAG 
CGTAACCCCC GGGAGAGACC CTCCGTCTCG ACCATTTTGG ACAAGCCTTT TCTTGCTCGC 
AGAATTCACA AGTTTCTCAG CCCTCAGCTT ATTGCTCAGG AATTCAGCCA TTCAATTCAC 
CTTCAGCCCA AAATGAGTGT GGCCCATGCA GCACCAGCCA AACGTCCAGC CCCAGGCCCT 
ATCCCAATCA CCTCAGCTCA GAAAATCACC AAACCAGCAG CCAAATACGG AGTGCCTTTA 
ACAGTGCGGA GACCATCTGA AGTCGCCAGG AAGGTCCCAG ATGCAGCAAA GAAACCAGTT 
AAACTTAAGC AGGCTCCCCC TCCTGCAGCG GTTCAGAGGA GAGTGAGTCG AGTGGAGGAG 
GAGAGGAGAA AGCATGAGGA AGGAGTGAGG AAGAAGCGGA TGGAGATGAT GGAAAGAGAG 
AGAAAACAAA GAGAACAGAT GTTGTTACTG AAGGCTGCTC AGATGAAGAG ATTTGAGAGA 
GAAAAGCTGA ACCGAATCAA CCGAGCTCGA GAGCAGGGCT GGAGACACGT GCTGAGTTCT 
AGTGGAGGAA GCAGTCCTGA GCGGAAGTTT TATGGAGGTG GCGGCGGCAA TGGAGCTCCC 
CATTCCCCAG GAATATCTCC CTTTCCTGTG CCTGGACCCT CCCCTGTTCC TTCTTCAGCT 
TTAGCTCCAA GCAGAGCGCC ATATGACCGC ATCCACCCTT CAGCTGAAAA ATTAACCAAA 
CCTACACCTA AAGATGGTGC TGGTGAAGGA GCCGTGCATG CAGGGGACAA TGGCAGGACT 
TCTTCAGACT CAGGGACTCT AAATGGTCAT GCCTGCATTC CTGTTAAGAA TGGCGTCAGG 
AAAGAACAAC GTAAAACAAG TCTCCAAAAT GAGCAGATTT CAAAGATCGA CTATGCCAAT 
AGGTTTCGAG GTCAGCTGGC AGTAGAGAAA TCCAAGCAGG TGGAGGAGTT TCTACAGCGG 
AAAAGAGAAG CCATGCTGAA TAAAGTTCGT GCAGAAGGAC AGCTGGGGGC CCGGCAAAAC 
CTGGCCGCCA TCTTTGCAAG CTGGACAAAC TCCTCCCGAT GCAGGAGACC CCGAGTGAAC 
AAAGAGGAGG AGGAGTATTT GACCAGATTA CGACAGATCA GACTACAGAA CTTTAATGAA 
CGTCAGCAGA TTAAAGCTCG GCTCAGAGGG GAGAAGTATG ACAGTGATGG CTCAGACAGT 
CAGGAGTCAT GTGAAGAGGC AGTGCTCAGG ATGAAGAAGA TTGAGGCTTT GAAGGCCCAG 
TCAAAGGCTC GTGCTGCTGT ACTGAAAGAG CAGCTGGAAA AGAAGAGAAG AGAAGCGTAT 
GAAAGAGAGA AGAAAGCATG GGAGGATCAT CAGCTTGTCG CTCGAGGAGT AAAAGTTGCA 
GTTGGGCCTG TGGGTGGGAC TCCCTCTCCT GCAGCCCCTC CAACTCCACC ATCTGACCCC 
ACCCCTCAGG AAGCTCCTTC TAAAGCCACA CCCTCCTCTA GACCCAGCAC TCCAGCCATC 
TCTATGACTG CTGCACTGAA GGATGTGGGC GCGATTTCAT CAGGGCAGAG TTCTCCTATG 
AAAGAGGACT CCCTTAAAGT TGAATCATCA GATGTACAGA GTGACAAGAA GGAGATTCTT 
CGCAGACTTA ATCAGAAGGT TCAGACCACT GAGGAAGTTT CTGTAAAAGA GCCCACACCC 
TCCAGCAATC AACCATGTCC TGATCCTCAG CCAGTCCAAC CCTCCATCAC AGAGGAAAGG 
CCTTCCACAG CTGCAGATAG GAAGAAGTGG GAAGCCGTAG CTCCACCCAT ACTGTCTGTT 
GCTGAACAGA CTCTCGAGGA AACCTGTGCT ACTCTGGCAG TGTCTCAGGT GGCATCACCA 
GAAACGAATA CTCCCTCGTG TGGAGACAGG AAGAAATGGC AGACAGATGG TGTTCCTCTT 
CTGTCTGTGG CTCAGCAGAC ACTGGAAGAA ACCAGCTTTA GAACAGCTGG TCAATTAACA 
GAAAAAACAG TAGGTGAAGT CATTTGCATG GATGCCTGGC AGGGGGAGGG GCTAAGGAAG 
GCTTGGGGGC AGAGTCCAGA TTCAGAGGTT TTGAAGGTGT TGGAAGAGGC AGAACTTCAG 
TCATTTACTC AGAGACTAGA TCCTGCCAGC ACGTGTGACC AAACACTGAC TGAAAGCCTT 
AATCCCATTG AGGAATCGGA TGCCACTAAT GAAGCCAAGC AAGTGCCTCC TCCTGAACCG 
TGCCAGCCTG CCACTGACCA TGACAAACAA GAAGTGACAT GTCAAGAGAC AGTGCCAATC 
ATAGAAGAGG TTAAAAGTCA GGAACTTATC CAAACAACAG CAGAGGGGAC AGAAAAATCT 
CATTCTGCAG AAAGTGACAT AGAGGTGGTG GATGATATGG AGTCAGTTGT TCTGGATGAT 
GTACCACAGA AAACCGCAGA GCCGCCCCCT GCAGAAGTAA TTCAGGCGTG GACTAAAGAC 
ACTCCAGAGT CTCTGCCAGC AGAGGGCAGA GTAATACAAG CAGAATGCAC AGAGGTACCT 
CAGAGAGAAG AGAAACATCC TGAACCTCCA GATGAGTCTT TGTTTGTGAA GTTAAGCAGT 
AGTCCAGCTC ACAGACGCAC AGTAGCGTTG GCCATGCTGT CTGCTCAATC CTCATTGGAA 
GACTCTTCCT CTGCTGCCTC ACGTTCTCGA TCAGTGTCTC CACTGCGCTC CCACCGCCAT 
GAAGATGCAC TGCTCATCGG CCTCTCCACT GGCCTCTTCG ATGCAAATAA CCCAAAGATG 
CTAAGGACGT GCTCCTTACC AGACCTCAGT AAGGTGTTTA GTGATGCTAC AGGACCTGAA 
GGTGCAGCCG CTCCTGACAG CAACCTAGAA ATTGAAGATC TGGAAGATAA AGCAGATGAG 
CAGTCTGAAC CTGAGGATGT GTATGAGGAT GACGATTTAA GAGAGCTCAG AGCATCTATG 
GAGAGGCTTC TTCAGGAGCA GCACAGTGAA GATGATGATG ATGAAGAAGA GGATGAGGGT 
GGTGGATCTA ATGGCAGCCC GGCCGATGAA GAGCCAGCTG GTCTTAATGG GTTAGCTAGT 
GCTGGTGATG ACAAACGCAG CCCAGAGGAA GAGACTTTTA ATGGAATGGC TGAAGATGAT 
GAGGATGTTA GCCATGGTGA AGAAGAACCA GGAGGCCCAG TCACAAATGG AATGGAATTT 
GAAGATGATG AAGAGCAGAA CAGCAGTGAA GCACAGCTCA ATGAGGAGTG GCAGTCAGAT 
GGCAGCGAGG AGGAGGACGA CGAAGAGCTG GAACAGCAGG ACAGCATCTT CAGTCGTCTG 
GAGGAGCTGC GGCTTCATCT AGAGCAGGCC ATGGGCTTTG AGAACTTCAT CCAGGCCTAC 
AACAAGATCA AGGCTATTCA TGAGGATGAG GATGAAAACA TTGCAATGGG CTCCAGCATG 
GTGCAAAACA TTTTGGGAAC TGAGCACCAG CACTTGTATC CTAAAATCCT CCATTTGGTG 
ATGGCAGATG GAGCTTACCA GGAAGATAAT GATGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_009294680.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009292955.1 Danio rerio NIMA-related kinase 1 (nek1), transcript variant X4, mRNA.

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CloneID	ODa42285
Clone ID Related Accession (Same CDS sequence)	XM_009294680.2 , XM_009294680.3
Accession Version	XM_009294680.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4176bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	serine/threonine-protein kinase Nek1 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009294680.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAAAT ATGAGAGGTT AAAGAAGATT GGGGAGGGAT CCTTTGGGAA AGCCATCTTG 
GTCAAGTCAA GGACAGATGG CCGACAATAT GTTATCAAGG AGATTGGTAT CTCCAGGATG 
TCGAACAAGG AACGACAGGA ATCACGTAAA GAGGTGGCAG TTCTGGCTAA CATGAGCCAT 
CCAAACATTG TCCAATACAA GGAATCCTTT GAAGAGAGTG GTTGTTTGTA CATTGTAATG 
GACTACTGTG AGGGAGGAGA CCTGTTTAAG AAGATCAATA ATCAAAGAGG ATCTCTTTTC 
CCAGAGGAAC AGATTCTTGA CTGGTTTGTG CAAATCTGTC TGGCTCTTAA ACATGTACAC 
GACCGCAAAA TCCTACATAG GGACATCAAA TCACAGAATA TTTTTCTGAC CAAAGATGGT 
ACTGTGCAGC TTGGAGACTT TGGAATCGCT AGAGTTTTGA ACAGCACCGT GGAGTTGGCA 
AGGACATGTA TCGGCACTCC TTATTACCTG TCGCCAGAGA TATGTGAAAA TAAACCATAC 
AACAACAAGA GTGATATCTG GGCCCTGGGA TGTGTCCTGT ATGAAATGTG CACGCTTAAG 
CATGCATTTG AAGCAGGAAA CATGAAGAAC CTGGTGCTGA AAATCATCCG TGGTTCATAC 
CCGCCTGTTT CCATCCATTA CTCCCCAGAT TTGCGCTCTC TCCTGGCCCA GCTCTTCAAG 
CGTAACCCCC GGGAGAGACC CTCCGTCTCG ACCATTTTGG ACAAGCCTTT TCTTGCTCGC 
AGAATTCACA AGTTTCTCAG CCCTCAGCTT ATTGCTCAGG AATTCAGCCA TTCAATTCAC 
CTTCAGCCCA AAATGAGTGT GGCCCATGCA GCACCAGCCA AACGTCCAGC CCCAGGCCCT 
ATCCCAATCA CCTCAGCTCA GAAAATCACC AAACCAGCAG CCAAATACGG AGTGCCTTTA 
ACAGTGCGGA GACCATCTGA AGTCGCCAGG AAGGTCCCAG ATGCAGCAAA GAAACCAGTT 
AAACTTAAGC AGGAAGGAGT GAGGAAGAAG CGGATGGAGA TGATGGAAAG AGAGAGAAAA 
CAAAGAGAAC AGATGTTGTT ACTGAAGGCT GCTCAGATGA AGAGATTTGA GAGAGAAAAG 
CTGAACCGAA TCAACCGAGC TCGAGAGCAG GGCTGGAGAC ACGTGCTGAG TTCTAGTGGA 
GGAAGCAGTC CTGAGCGGAA GTTTTATGGA GGTGGCGGCG GCAATGGAGC TCCCCATTCC 
CCAGGAATAT CTCCCTTTCC TGTGCCTGGA CCCTCCCCTG TTCCTTCTTC AGCTTTAGCT 
CCAAGCAGAG CGCCATATGA CCGCATCCAC CCTTCAGCTG AAAAATTAAC CAAACCTACA 
CCTAAAGATG GTGCTGGTGA AGGAGCCGTG CATGCAGGGG ACAATGGCAG GACTTCTTCA 
GACTCAGGGA CTCTAAATGG TCATGCCTGC ATTCCTGTTA AGAATGGCGT CAGGAAAGAA 
CAACGTAAAA CAAGTCTCCA AAATGAGCAG ATTTCAAAGA TCGACTATGC CAATAGGTTT 
CGAGGTCAGC TGGCAGTAGA GAAATCCAAG CAGGTGGAGG AGTTTCTACA GCGGAAAAGA 
GAAGCCATGC TGAATAAAGT TCGTGCAGAA GGACAGCTGG GGGCCCGGCA AAACCTGGCC 
GCCATCTTTG CAAGCTGGAC AAACTCCTCC CGATGCAGGA GACCCCGAGT GAACAAAGAG 
GAGGAGGAGT ATTTGACCAG ATTACGACAG ATCAGACTAC AGAACTTTAA TGAACGTCAG 
CAGATTAAAG CTCGGCTCAG AGGGGAGAAG TATGACAGTG ATGGCTCAGA CAGTCAGGAG 
TCATGTGAAG AGGCAGTGCT CAGGATGAAG AAGATTGAGG CTTTGAAGGC CCAGTCAAAG 
GCTCGTGCTG CTGTACTGAA AGAGCAGCTG GAAAAGAAGA GAAGAGAAGC GTATGAAAGA 
GAGAAGAAAG CATGGGAGGA TCATCAGCTT GTCGCTCGAG GAGTAAAAGT TGCAGTTGGG 
CCTGTGGGTG GGACTCCCTC TCCTGCAGCC CCTCCAACTC CACCATCTGA CCCCACCCCT 
CAGGAAGCTC CTTCTAAAGC CACACCCTCC TCTAGACCCA GCACTCCAGC CATCTCTATG 
ACTGCTGCAC TGAAGGATGT GGGCGCGATT TCATCAGGGC AGAGTTCTCC TATGAAAGAG 
GACTCCCTTA AAGTTGAATC ATCAGATGTA CAGAGTGACA AGAAGGAGAT TCTTCGCAGA 
CTTAATCAGA AGGTTCAGAC CACTGAGGAA GTTTCTGTAA AAGAGCCCAC ACCCTCCAGC 
AATCAACCAT GTCCTGATCC TCAGCCAGTC CAACCCTCCA TCACAGAGGA AAGGCCTTCC 
ACAGCTGCAG ATAGGAAGAA GTGGGAAGCC GTAGCTCCAC CCATACTGTC TGTTGCTGAA 
CAGACTCTCG AGGAAACCTG TGCTACTCTG GCAGTGTCTC AGGTGGCATC ACCAGAAACG 
AATACTCCCT CGTGTGGAGA CAGGAAGAAA TGGCAGACAG ATGGTGTTCC TCTTCTGTCT 
GTGGCTCAGC AGACACTGGA AGAAACCAGC TTTAGAACAG CTGGTCAATT AACAGAAAAA 
ACAGTAGGTG AAGTCATTTG CATGGATGCC TGGCAGGGGG AGGGGCTAAG GAAGGCTTGG 
GGGCAGAGTC CAGATTCAGA GGTTTTGAAG GTGTTGGAAG AGGCAGAACT TCAGTCATTT 
ACTCAGAGAC TAGATCCTGC CAGCACGTGT GACCAAACAC TGACTGAAAG CCTTAATCCC 
ATTGAGGAAT CGGATGCCAC TAATGAAGCC AAGCAAGTGC CTCCTCCTGA ACCGTGCCAG 
CCTGCCACTG ACCATGACAA ACAAGAAGTG ACATGTCAAG AGACAGTGCC AATCATAGAA 
GAGGTTAAAA GTCAGGAACT TATCCAAACA ACAGCAGAGG GGACAGAAAA ATCTCATTCT 
GCAGAAAGTG ACATAGAGGT GGTGGATGAT ATGGAGTCAG TTGTTCTGGA TGATGTACCA 
CAGAAAACCG CAGAGCCGCC CCCTGCAGAA GTAATTCAGG CGTGGACTAA AGACACTCCA 
GAGTCTCTGC CAGCAGAGGG CAGAGTAATA CAAGCAGAAT GCACAGAGGT ACCTCAGAGA 
GAAGAGAAAC ATCCTGAACC TCCAGCAGAT GAGTCTTTGT TTGTGAAGTT AAGCAGTAGT 
CCAGCTCACA GACGCACAGT AGCGTTGGCC ATGCTGTCTG CTCAATCCTC ATTGGAAGAC 
TCTTCCTCTG CTGCCTCACG TTCTCGATCA GTGTCTCCAC TGCGCTCCCA CCGCCATGAA 
GATGCACTGC TCATCGGCCT CTCCACTGGC CTCTTCGATG CAAATAACCC AAAGATGCTA 
AGGACGTGCT CCTTACCAGA CCTCAGTAAG GTGTTTAGTG ATGCTACAGG ACCTGAAGGT 
GCAGCCGCTC CTGACAGCAA CCTAGAAATT GAAGATCTGG AAGATAAAGC AGATGAGCAG 
TCTGAACCTG AGGATGTGTA TGAGGATGAC GATTTAAGAG AGCTCAGAGC ATCTATGGAG 
AGGCTTCTTC AGGAGCAGCA CAGTGAAGAT GATGATGATG AAGAAGAGGA TGAGGGTGGT 
GGATCTAATG GCAGCCCGGC CGATGAAGAG CCAGCTGGTC TTAATGGGTT AGCTAGTGCT 
GGTGATGACA AACGCAGCCC AGAGGAAGAG ACTTTTAATG GAATGGCTGA AGATGATGAG 
GATGTTAGCC ATGGTGAAGA AGAACCAGGA GGCCCAGTCA CAAATGGAAT GGAATTTGAA 
GATGATGAAG AGCAGAACAG CAGTGAAGCA CAGCTCAATG AGGAGTGGCA GTCAGATGGC 
AGCGAGGAGG AGGACGACGA AGAGCTGGAA CAGCAGGACA GCATCTTCAG TCGTCTGGAG 
GAGCTGCGGC TTCATCTAGA GCAGGCCATG GGCTTTGAGA ACTTCATCCA GGCCTACAAC 
AAGATCAAGG CTATTCATGA GGATGAGGAT GAAAACATTG CAATGGGCTC CAGCATGGTG 
CAAAACATTT TGGGAACTGA GCACCAGCAC TTGTATCCTA AAATCCTCCA TTTGGTGATG 
GCAGATGGAG CTTACCAGGA AGATAATGAT GAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009292955.1
CDS	118..4293
Translation	MDKYERLKKIGEGSFGKAILVKSRTDGRQYVIKEIGISRMSNKERQESRKEVAVLANMSHPNIVQYKESFEESGCLYIVMDYCEGGDLFKKINNQRGSLFPEEQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIIRGSYPPVSIHYSPDLRSLLAQLFKRNPRERPSVSTILDKPFLARRIHKFLSPQLIAQEFSHSIHLQPKMSVAHAAPAKRPAPGPIPITSAQKITKPAAKYGVPLTVRRPSEVARKVPDAAKKPVKLKQEGVRKKRMEMMERERKQREQMLLLKAAQMKRFEREKLNRINRAREQGWRHVLSSSGGSSPERKFYGGGGGNGAPHSPGISPFPVPGPSPVPSSALAPSRAPYDRIHPSAEKLTKPTPKDGAGEGAVHAGDNGRTSSDSGTLNGHACIPVKNGVRKEQRKTSLQNEQISKIDYANRFRGQLAVEKSKQVEEFLQRKREAMLNKVRAEGQLGARQNLAAIFASWTNSSRCRRPRVNKEEEEYLTRLRQIRLQNFNERQQIKARLRGEKYDSDGSDSQESCEEAVLRMKKIEALKAQSKARAAVLKEQLEKKRREAYEREKKAWEDHQLVARGVKVAVGPVGGTPSPAAPPTPPSDPTPQEAPSKATPSSRPSTPAISMTAALKDVGAISSGQSSPMKEDSLKVESSDVQSDKKEILRRLNQKVQTTEEVSVKEPTPSSNQPCPDPQPVQPSITEERPSTAADRKKWEAVAPPILSVAEQTLEETCATLAVSQVASPETNTPSCGDRKKWQTDGVPLLSVAQQTLEETSFRTAGQLTEKTVGEVICMDAWQGEGLRKAWGQSPDSEVLKVLEEAELQSFTQRLDPASTCDQTLTESLNPIEESDATNEAKQVPPPEPCQPATDHDKQEVTCQETVPIIEEVKSQELIQTTAEGTEKSHSAESDIEVVDDMESVVLDDVPQKTAEPPPAEVIQAWTKDTPESLPAEGRVIQAECTEVPQREEKHPEPPADESLFVKLSSSPAHRRTVALAMLSAQSSLEDSSSAASRSRSVSPLRSHRHEDALLIGLSTGLFDANNPKMLRTCSLPDLSKVFSDATGPEGAAAPDSNLEIEDLEDKADEQSEPEDVYEDDDLRELRASMERLLQEQHSEDDDDEEEDEGGGSNGSPADEEPAGLNGLASAGDDKRSPEEETFNGMAEDDEDVSHGEEEPGGPVTNGMEFEDDEEQNSSEAQLNEEWQSDGSEEEDDEELEQQDSIFSRLEELRLHLEQAMGFENFIQAYNKIKAIHEDEDENIAMGSSMVQNILGTEHQHLYPKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_009294680.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio NIMA-related kinase 1 (nek1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009294680.2

ORF Insert Sequence:

ATGGATAAAT ATGAGAGGTT AAAGAAGATT GGGGAGGGAT CCTTTGGGAA AGCCATCTTG 
GTCAAGTCAA GGACAGATGG CCGACAATAT GTTATCAAGG AGATTGGTAT CTCCAGGATG 
TCGAACAAGG AACGACAGGA ATCACGTAAA GAGGTGGCAG TTCTGGCTAA CATGAGCCAT 
CCAAACATTG TCCAATACAA GGAATCCTTT GAAGAGAGTG GTTGTTTGTA CATTGTAATG 
GACTACTGTG AGGGAGGAGA CCTGTTTAAG AAGATCAATA ATCAAAGAGG ATCTCTTTTC 
CCAGAGGAAC AGATTCTTGA CTGGTTTGTG CAAATCTGTC TGGCTCTTAA ACATGTACAC 
GACCGCAAAA TCCTACATAG GGACATCAAA TCACAGAATA TTTTTCTGAC CAAAGATGGT 
ACTGTGCAGC TTGGAGACTT TGGAATCGCT AGAGTTTTGA ACAGCACCGT GGAGTTGGCA 
AGGACATGTA TCGGCACTCC TTATTACCTG TCGCCAGAGA TATGTGAAAA TAAACCATAC 
AACAACAAGA GTGATATCTG GGCCCTGGGA TGTGTCCTGT ATGAAATGTG CACGCTTAAG 
CATGCATTTG AAGCAGGAAA CATGAAGAAC CTGGTGCTGA AAATCATCCG TGGTTCATAC 
CCGCCTGTTT CCATCCATTA CTCCCCAGAT TTGCGCTCTC TCCTGGCCCA GCTCTTCAAG 
CGTAACCCCC GGGAGAGACC CTCCGTCTCG ACCATTTTGG ACAAGCCTTT TCTTGCTCGC 
AGAATTCACA AGTTTCTCAG CCCTCAGCTT ATTGCTCAGG AATTCAGCCA TTCAATTCAC 
CTTCAGCCCA AAATGAGTGT GGCCCATGCA GCACCAGCCA AACGTCCAGC CCCAGGCCCT 
ATCCCAATCA CCTCAGCTCA GAAAATCACC AAACCAGCAG CCAAATACGG AGTGCCTTTA 
ACAGTGCGGA GACCATCTGA AGTCGCCAGG AAGGTCCCAG ATGCAGCAAA GAAACCAGTT 
AAACTTAAGC AGGAAGGAGT GAGGAAGAAG CGGATGGAGA TGATGGAAAG AGAGAGAAAA 
CAAAGAGAAC AGATGTTGTT ACTGAAGGCT GCTCAGATGA AGAGATTTGA GAGAGAAAAG 
CTGAACCGAA TCAACCGAGC TCGAGAGCAG GGCTGGAGAC ACGTGCTGAG TTCTAGTGGA 
GGAAGCAGTC CTGAGCGGAA GTTTTATGGA GGTGGCGGCG GCAATGGAGC TCCCCATTCC 
CCAGGAATAT CTCCCTTTCC TGTGCCTGGA CCCTCCCCTG TTCCTTCTTC AGCTTTAGCT 
CCAAGCAGAG CGCCATATGA CCGCATCCAC CCTTCAGCTG AAAAATTAAC CAAACCTACA 
CCTAAAGATG GTGCTGGTGA AGGAGCCGTG CATGCAGGGG ACAATGGCAG GACTTCTTCA 
GACTCAGGGA CTCTAAATGG TCATGCCTGC ATTCCTGTTA AGAATGGCGT CAGGAAAGAA 
CAACGTAAAA CAAGTCTCCA AAATGAGCAG ATTTCAAAGA TCGACTATGC CAATAGGTTT 
CGAGGTCAGC TGGCAGTAGA GAAATCCAAG CAGGTGGAGG AGTTTCTACA GCGGAAAAGA 
GAAGCCATGC TGAATAAAGT TCGTGCAGAA GGACAGCTGG GGGCCCGGCA AAACCTGGCC 
GCCATCTTTG CAAGCTGGAC AAACTCCTCC CGATGCAGGA GACCCCGAGT GAACAAAGAG 
GAGGAGGAGT ATTTGACCAG ATTACGACAG ATCAGACTAC AGAACTTTAA TGAACGTCAG 
CAGATTAAAG CTCGGCTCAG AGGGGAGAAG TATGACAGTG ATGGCTCAGA CAGTCAGGAG 
TCATGTGAAG AGGCAGTGCT CAGGATGAAG AAGATTGAGG CTTTGAAGGC CCAGTCAAAG 
GCTCGTGCTG CTGTACTGAA AGAGCAGCTG GAAAAGAAGA GAAGAGAAGC GTATGAAAGA 
GAGAAGAAAG CATGGGAGGA TCATCAGCTT GTCGCTCGAG GAGTAAAAGT TGCAGTTGGG 
CCTGTGGGTG GGACTCCCTC TCCTGCAGCC CCTCCAACTC CACCATCTGA CCCCACCCCT 
CAGGAAGCTC CTTCTAAAGC CACACCCTCC TCTAGACCCA GCACTCCAGC CATCTCTATG 
ACTGCTGCAC TGAAGGATGT GGGCGCGATT TCATCAGGGC AGAGTTCTCC TATGAAAGAG 
GACTCCCTTA AAGTTGAATC ATCAGATGTA CAGAGTGACA AGAAGGAGAT TCTTCGCAGA 
CTTAATCAGA AGGTTCAGAC CACTGAGGAA GTTTCTGTAA AAGAGCCCAC ACCCTCCAGC 
AATCAACCAT GTCCTGATCC TCAGCCAGTC CAACCCTCCA TCACAGAGGA AAGGCCTTCC 
ACAGCTGCAG ATAGGAAGAA GTGGGAAGCC GTAGCTCCAC CCATACTGTC TGTTGCTGAA 
CAGACTCTCG AGGAAACCTG TGCTACTCTG GCAGTGTCTC AGGTGGCATC ACCAGAAACG 
AATACTCCCT CGTGTGGAGA CAGGAAGAAA TGGCAGACAG ATGGTGTTCC TCTTCTGTCT 
GTGGCTCAGC AGACACTGGA AGAAACCAGC TTTAGAACAG CTGGTCAATT AACAGAAAAA 
ACAGTAGGTG AAGTCATTTG CATGGATGCC TGGCAGGGGG AGGGGCTAAG GAAGGCTTGG 
GGGCAGAGTC CAGATTCAGA GGTTTTGAAG GTGTTGGAAG AGGCAGAACT TCAGTCATTT 
ACTCAGAGAC TAGATCCTGC CAGCACGTGT GACCAAACAC TGACTGAAAG CCTTAATCCC 
ATTGAGGAAT CGGATGCCAC TAATGAAGCC AAGCAAGTGC CTCCTCCTGA ACCGTGCCAG 
CCTGCCACTG ACCATGACAA ACAAGAAGTG ACATGTCAAG AGACAGTGCC AATCATAGAA 
GAGGTTAAAA GTCAGGAACT TATCCAAACA ACAGCAGAGG GGACAGAAAA ATCTCATTCT 
GCAGAAAGTG ACATAGAGGT GGTGGATGAT ATGGAGTCAG TTGTTCTGGA TGATGTACCA 
CAGAAAACCG CAGAGCCGCC CCCTGCAGAA GTAATTCAGG CGTGGACTAA AGACACTCCA 
GAGTCTCTGC CAGCAGAGGG CAGAGTAATA CAAGCAGAAT GCACAGAGGT ACCTCAGAGA 
GAAGAGAAAC ATCCTGAACC TCCAGCAGAT GAGTCTTTGT TTGTGAAGTT AAGCAGTAGT 
CCAGCTCACA GACGCACAGT AGCGTTGGCC ATGCTGTCTG CTCAATCCTC ATTGGAAGAC 
TCTTCCTCTG CTGCCTCACG TTCTCGATCA GTGTCTCCAC TGCGCTCCCA CCGCCATGAA 
GATGCACTGC TCATCGGCCT CTCCACTGGC CTCTTCGATG CAAATAACCC AAAGATGCTA 
AGGACGTGCT CCTTACCAGA CCTCAGTAAG GTGTTTAGTG ATGCTACAGG ACCTGAAGGT 
GCAGCCGCTC CTGACAGCAA CCTAGAAATT GAAGATCTGG AAGATAAAGC AGATGAGCAG 
TCTGAACCTG AGGATGTGTA TGAGGATGAC GATTTAAGAG AGCTCAGAGC ATCTATGGAG 
AGGCTTCTTC AGGAGCAGCA CAGTGAAGAT GATGATGATG AAGAAGAGGA TGAGGGTGGT 
GGATCTAATG GCAGCCCGGC CGATGAAGAG CCAGCTGGTC TTAATGGGTT AGCTAGTGCT 
GGTGATGACA AACGCAGCCC AGAGGAAGAG ACTTTTAATG GAATGGCTGA AGATGATGAG 
GATGTTAGCC ATGGTGAAGA AGAACCAGGA GGCCCAGTCA CAAATGGAAT GGAATTTGAA 
GATGATGAAG AGCAGAACAG CAGTGAAGCA CAGCTCAATG AGGAGTGGCA GTCAGATGGC 
AGCGAGGAGG AGGACGACGA AGAGCTGGAA CAGCAGGACA GCATCTTCAG TCGTCTGGAG 
GAGCTGCGGC TTCATCTAGA GCAGGCCATG GGCTTTGAGA ACTTCATCCA GGCCTACAAC 
AAGATCAAGG CTATTCATGA GGATGAGGAT GAAAACATTG CAATGGGCTC CAGCATGGTG 
CAAAACATTT TGGGAACTGA GCACCAGCAC TTGTATCCTA AAATCCTCCA TTTGGTGATG 
GCAGATGGAG CTTACCAGGA AGATAATGAT GAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_009294680.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009292955.1 Danio rerio NIMA-related kinase 1 (nek1), transcript variant X4, mRNA.

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CloneID	ODa42285
Clone ID Related Accession (Same CDS sequence)	XM_009294680.2 , XM_009294680.3
Accession Version	XM_009294680.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4176bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase Nek1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009294680.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAAAT ATGAGAGGTT AAAGAAGATT GGGGAGGGAT CCTTTGGGAA AGCCATCTTG 
GTCAAGTCAA GGACAGATGG CCGACAATAT GTTATCAAGG AGATTGGTAT CTCCAGGATG 
TCGAACAAGG AACGACAGGA ATCACGTAAA GAGGTGGCAG TTCTGGCTAA CATGAGCCAT 
CCAAACATTG TCCAATACAA GGAATCCTTT GAAGAGAGTG GTTGTTTGTA CATTGTAATG 
GACTACTGTG AGGGAGGAGA CCTGTTTAAG AAGATCAATA ATCAAAGAGG ATCTCTTTTC 
CCAGAGGAAC AGATTCTTGA CTGGTTTGTG CAAATCTGTC TGGCTCTTAA ACATGTACAC 
GACCGCAAAA TCCTACATAG GGACATCAAA TCACAGAATA TTTTTCTGAC CAAAGATGGT 
ACTGTGCAGC TTGGAGACTT TGGAATCGCT AGAGTTTTGA ACAGCACCGT GGAGTTGGCA 
AGGACATGTA TCGGCACTCC TTATTACCTG TCGCCAGAGA TATGTGAAAA TAAACCATAC 
AACAACAAGA GTGATATCTG GGCCCTGGGA TGTGTCCTGT ATGAAATGTG CACGCTTAAG 
CATGCATTTG AAGCAGGAAA CATGAAGAAC CTGGTGCTGA AAATCATCCG TGGTTCATAC 
CCGCCTGTTT CCATCCATTA CTCCCCAGAT TTGCGCTCTC TCCTGGCCCA GCTCTTCAAG 
CGTAACCCCC GGGAGAGACC CTCCGTCTCG ACCATTTTGG ACAAGCCTTT TCTTGCTCGC 
AGAATTCACA AGTTTCTCAG CCCTCAGCTT ATTGCTCAGG AATTCAGCCA TTCAATTCAC 
CTTCAGCCCA AAATGAGTGT GGCCCATGCA GCACCAGCCA AACGTCCAGC CCCAGGCCCT 
ATCCCAATCA CCTCAGCTCA GAAAATCACC AAACCAGCAG CCAAATACGG AGTGCCTTTA 
ACAGTGCGGA GACCATCTGA AGTCGCCAGG AAGGTCCCAG ATGCAGCAAA GAAACCAGTT 
AAACTTAAGC AGGAAGGAGT GAGGAAGAAG CGGATGGAGA TGATGGAAAG AGAGAGAAAA 
CAAAGAGAAC AGATGTTGTT ACTGAAGGCT GCTCAGATGA AGAGATTTGA GAGAGAAAAG 
CTGAACCGAA TCAACCGAGC TCGAGAGCAG GGCTGGAGAC ACGTGCTGAG TTCTAGTGGA 
GGAAGCAGTC CTGAGCGGAA GTTTTATGGA GGTGGCGGCG GCAATGGAGC TCCCCATTCC 
CCAGGAATAT CTCCCTTTCC TGTGCCTGGA CCCTCCCCTG TTCCTTCTTC AGCTTTAGCT 
CCAAGCAGAG CGCCATATGA CCGCATCCAC CCTTCAGCTG AAAAATTAAC CAAACCTACA 
CCTAAAGATG GTGCTGGTGA AGGAGCCGTG CATGCAGGGG ACAATGGCAG GACTTCTTCA 
GACTCAGGGA CTCTAAATGG TCATGCCTGC ATTCCTGTTA AGAATGGCGT CAGGAAAGAA 
CAACGTAAAA CAAGTCTCCA AAATGAGCAG ATTTCAAAGA TCGACTATGC CAATAGGTTT 
CGAGGTCAGC TGGCAGTAGA GAAATCCAAG CAGGTGGAGG AGTTTCTACA GCGGAAAAGA 
GAAGCCATGC TGAATAAAGT TCGTGCAGAA GGACAGCTGG GGGCCCGGCA AAACCTGGCC 
GCCATCTTTG CAAGCTGGAC AAACTCCTCC CGATGCAGGA GACCCCGAGT GAACAAAGAG 
GAGGAGGAGT ATTTGACCAG ATTACGACAG ATCAGACTAC AGAACTTTAA TGAACGTCAG 
CAGATTAAAG CTCGGCTCAG AGGGGAGAAG TATGACAGTG ATGGCTCAGA CAGTCAGGAG 
TCATGTGAAG AGGCAGTGCT CAGGATGAAG AAGATTGAGG CTTTGAAGGC CCAGTCAAAG 
GCTCGTGCTG CTGTACTGAA AGAGCAGCTG GAAAAGAAGA GAAGAGAAGC GTATGAAAGA 
GAGAAGAAAG CATGGGAGGA TCATCAGCTT GTCGCTCGAG GAGTAAAAGT TGCAGTTGGG 
CCTGTGGGTG GGACTCCCTC TCCTGCAGCC CCTCCAACTC CACCATCTGA CCCCACCCCT 
CAGGAAGCTC CTTCTAAAGC CACACCCTCC TCTAGACCCA GCACTCCAGC CATCTCTATG 
ACTGCTGCAC TGAAGGATGT GGGCGCGATT TCATCAGGGC AGAGTTCTCC TATGAAAGAG 
GACTCCCTTA AAGTTGAATC ATCAGATGTA CAGAGTGACA AGAAGGAGAT TCTTCGCAGA 
CTTAATCAGA AGGTTCAGAC CACTGAGGAA GTTTCTGTAA AAGAGCCCAC ACCCTCCAGC 
AATCAACCAT GTCCTGATCC TCAGCCAGTC CAACCCTCCA TCACAGAGGA AAGGCCTTCC 
ACAGCTGCAG ATAGGAAGAA GTGGGAAGCC GTAGCTCCAC CCATACTGTC TGTTGCTGAA 
CAGACTCTCG AGGAAACCTG TGCTACTCTG GCAGTGTCTC AGGTGGCATC ACCAGAAACG 
AATACTCCCT CGTGTGGAGA CAGGAAGAAA TGGCAGACAG ATGGTGTTCC TCTTCTGTCT 
GTGGCTCAGC AGACACTGGA AGAAACCAGC TTTAGAACAG CTGGTCAATT AACAGAAAAA 
ACAGTAGGTG AAGTCATTTG CATGGATGCC TGGCAGGGGG AGGGGCTAAG GAAGGCTTGG 
GGGCAGAGTC CAGATTCAGA GGTTTTGAAG GTGTTGGAAG AGGCAGAACT TCAGTCATTT 
ACTCAGAGAC TAGATCCTGC CAGCACGTGT GACCAAACAC TGACTGAAAG CCTTAATCCC 
ATTGAGGAAT CGGATGCCAC TAATGAAGCC AAGCAAGTGC CTCCTCCTGA ACCGTGCCAG 
CCTGCCACTG ACCATGACAA ACAAGAAGTG ACATGTCAAG AGACAGTGCC AATCATAGAA 
GAGGTTAAAA GTCAGGAACT TATCCAAACA ACAGCAGAGG GGACAGAAAA ATCTCATTCT 
GCAGAAAGTG ACATAGAGGT GGTGGATGAT ATGGAGTCAG TTGTTCTGGA TGATGTACCA 
CAGAAAACCG CAGAGCCGCC CCCTGCAGAA GTAATTCAGG CGTGGACTAA AGACACTCCA 
GAGTCTCTGC CAGCAGAGGG CAGAGTAATA CAAGCAGAAT GCACAGAGGT ACCTCAGAGA 
GAAGAGAAAC ATCCTGAACC TCCAGCAGAT GAGTCTTTGT TTGTGAAGTT AAGCAGTAGT 
CCAGCTCACA GACGCACAGT AGCGTTGGCC ATGCTGTCTG CTCAATCCTC ATTGGAAGAC 
TCTTCCTCTG CTGCCTCACG TTCTCGATCA GTGTCTCCAC TGCGCTCCCA CCGCCATGAA 
GATGCACTGC TCATCGGCCT CTCCACTGGC CTCTTCGATG CAAATAACCC AAAGATGCTA 
AGGACGTGCT CCTTACCAGA CCTCAGTAAG GTGTTTAGTG ATGCTACAGG ACCTGAAGGT 
GCAGCCGCTC CTGACAGCAA CCTAGAAATT GAAGATCTGG AAGATAAAGC AGATGAGCAG 
TCTGAACCTG AGGATGTGTA TGAGGATGAC GATTTAAGAG AGCTCAGAGC ATCTATGGAG 
AGGCTTCTTC AGGAGCAGCA CAGTGAAGAT GATGATGATG AAGAAGAGGA TGAGGGTGGT 
GGATCTAATG GCAGCCCGGC CGATGAAGAG CCAGCTGGTC TTAATGGGTT AGCTAGTGCT 
GGTGATGACA AACGCAGCCC AGAGGAAGAG ACTTTTAATG GAATGGCTGA AGATGATGAG 
GATGTTAGCC ATGGTGAAGA AGAACCAGGA GGCCCAGTCA CAAATGGAAT GGAATTTGAA 
GATGATGAAG AGCAGAACAG CAGTGAAGCA CAGCTCAATG AGGAGTGGCA GTCAGATGGC 
AGCGAGGAGG AGGACGACGA AGAGCTGGAA CAGCAGGACA GCATCTTCAG TCGTCTGGAG 
GAGCTGCGGC TTCATCTAGA GCAGGCCATG GGCTTTGAGA ACTTCATCCA GGCCTACAAC 
AAGATCAAGG CTATTCATGA GGATGAGGAT GAAAACATTG CAATGGGCTC CAGCATGGTG 
CAAAACATTT TGGGAACTGA GCACCAGCAC TTGTATCCTA AAATCCTCCA TTTGGTGATG 
GCAGATGGAG CTTACCAGGA AGATAATGAT GAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009292955.1
CDS	127..4302
Translation	MDKYERLKKIGEGSFGKAILVKSRTDGRQYVIKEIGISRMSNKERQESRKEVAVLANMSHPNIVQYKESFEESGCLYIVMDYCEGGDLFKKINNQRGSLFPEEQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIIRGSYPPVSIHYSPDLRSLLAQLFKRNPRERPSVSTILDKPFLARRIHKFLSPQLIAQEFSHSIHLQPKMSVAHAAPAKRPAPGPIPITSAQKITKPAAKYGVPLTVRRPSEVARKVPDAAKKPVKLKQEGVRKKRMEMMERERKQREQMLLLKAAQMKRFEREKLNRINRAREQGWRHVLSSSGGSSPERKFYGGGGGNGAPHSPGISPFPVPGPSPVPSSALAPSRAPYDRIHPSAEKLTKPTPKDGAGEGAVHAGDNGRTSSDSGTLNGHACIPVKNGVRKEQRKTSLQNEQISKIDYANRFRGQLAVEKSKQVEEFLQRKREAMLNKVRAEGQLGARQNLAAIFASWTNSSRCRRPRVNKEEEEYLTRLRQIRLQNFNERQQIKARLRGEKYDSDGSDSQESCEEAVLRMKKIEALKAQSKARAAVLKEQLEKKRREAYEREKKAWEDHQLVARGVKVAVGPVGGTPSPAAPPTPPSDPTPQEAPSKATPSSRPSTPAISMTAALKDVGAISSGQSSPMKEDSLKVESSDVQSDKKEILRRLNQKVQTTEEVSVKEPTPSSNQPCPDPQPVQPSITEERPSTAADRKKWEAVAPPILSVAEQTLEETCATLAVSQVASPETNTPSCGDRKKWQTDGVPLLSVAQQTLEETSFRTAGQLTEKTVGEVICMDAWQGEGLRKAWGQSPDSEVLKVLEEAELQSFTQRLDPASTCDQTLTESLNPIEESDATNEAKQVPPPEPCQPATDHDKQEVTCQETVPIIEEVKSQELIQTTAEGTEKSHSAESDIEVVDDMESVVLDDVPQKTAEPPPAEVIQAWTKDTPESLPAEGRVIQAECTEVPQREEKHPEPPADESLFVKLSSSPAHRRTVALAMLSAQSSLEDSSSAASRSRSVSPLRSHRHEDALLIGLSTGLFDANNPKMLRTCSLPDLSKVFSDATGPEGAAAPDSNLEIEDLEDKADEQSEPEDVYEDDDLRELRASMERLLQEQHSEDDDDEEEDEGGGSNGSPADEEPAGLNGLASAGDDKRSPEEETFNGMAEDDEDVSHGEEEPGGPVTNGMEFEDDEEQNSSEAQLNEEWQSDGSEEEDDEELEQQDSIFSRLEELRLHLEQAMGFENFIQAYNKIKAIHEDEDENIAMGSSMVQNILGTEHQHLYPKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_009294680.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio NIMA-related kinase 1 (nek1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009294680.3

ORF Insert Sequence:

ATGGATAAAT ATGAGAGGTT AAAGAAGATT GGGGAGGGAT CCTTTGGGAA AGCCATCTTG 
GTCAAGTCAA GGACAGATGG CCGACAATAT GTTATCAAGG AGATTGGTAT CTCCAGGATG 
TCGAACAAGG AACGACAGGA ATCACGTAAA GAGGTGGCAG TTCTGGCTAA CATGAGCCAT 
CCAAACATTG TCCAATACAA GGAATCCTTT GAAGAGAGTG GTTGTTTGTA CATTGTAATG 
GACTACTGTG AGGGAGGAGA CCTGTTTAAG AAGATCAATA ATCAAAGAGG ATCTCTTTTC 
CCAGAGGAAC AGATTCTTGA CTGGTTTGTG CAAATCTGTC TGGCTCTTAA ACATGTACAC 
GACCGCAAAA TCCTACATAG GGACATCAAA TCACAGAATA TTTTTCTGAC CAAAGATGGT 
ACTGTGCAGC TTGGAGACTT TGGAATCGCT AGAGTTTTGA ACAGCACCGT GGAGTTGGCA 
AGGACATGTA TCGGCACTCC TTATTACCTG TCGCCAGAGA TATGTGAAAA TAAACCATAC 
AACAACAAGA GTGATATCTG GGCCCTGGGA TGTGTCCTGT ATGAAATGTG CACGCTTAAG 
CATGCATTTG AAGCAGGAAA CATGAAGAAC CTGGTGCTGA AAATCATCCG TGGTTCATAC 
CCGCCTGTTT CCATCCATTA CTCCCCAGAT TTGCGCTCTC TCCTGGCCCA GCTCTTCAAG 
CGTAACCCCC GGGAGAGACC CTCCGTCTCG ACCATTTTGG ACAAGCCTTT TCTTGCTCGC 
AGAATTCACA AGTTTCTCAG CCCTCAGCTT ATTGCTCAGG AATTCAGCCA TTCAATTCAC 
CTTCAGCCCA AAATGAGTGT GGCCCATGCA GCACCAGCCA AACGTCCAGC CCCAGGCCCT 
ATCCCAATCA CCTCAGCTCA GAAAATCACC AAACCAGCAG CCAAATACGG AGTGCCTTTA 
ACAGTGCGGA GACCATCTGA AGTCGCCAGG AAGGTCCCAG ATGCAGCAAA GAAACCAGTT 
AAACTTAAGC AGGAAGGAGT GAGGAAGAAG CGGATGGAGA TGATGGAAAG AGAGAGAAAA 
CAAAGAGAAC AGATGTTGTT ACTGAAGGCT GCTCAGATGA AGAGATTTGA GAGAGAAAAG 
CTGAACCGAA TCAACCGAGC TCGAGAGCAG GGCTGGAGAC ACGTGCTGAG TTCTAGTGGA 
GGAAGCAGTC CTGAGCGGAA GTTTTATGGA GGTGGCGGCG GCAATGGAGC TCCCCATTCC 
CCAGGAATAT CTCCCTTTCC TGTGCCTGGA CCCTCCCCTG TTCCTTCTTC AGCTTTAGCT 
CCAAGCAGAG CGCCATATGA CCGCATCCAC CCTTCAGCTG AAAAATTAAC CAAACCTACA 
CCTAAAGATG GTGCTGGTGA AGGAGCCGTG CATGCAGGGG ACAATGGCAG GACTTCTTCA 
GACTCAGGGA CTCTAAATGG TCATGCCTGC ATTCCTGTTA AGAATGGCGT CAGGAAAGAA 
CAACGTAAAA CAAGTCTCCA AAATGAGCAG ATTTCAAAGA TCGACTATGC CAATAGGTTT 
CGAGGTCAGC TGGCAGTAGA GAAATCCAAG CAGGTGGAGG AGTTTCTACA GCGGAAAAGA 
GAAGCCATGC TGAATAAAGT TCGTGCAGAA GGACAGCTGG GGGCCCGGCA AAACCTGGCC 
GCCATCTTTG CAAGCTGGAC AAACTCCTCC CGATGCAGGA GACCCCGAGT GAACAAAGAG 
GAGGAGGAGT ATTTGACCAG ATTACGACAG ATCAGACTAC AGAACTTTAA TGAACGTCAG 
CAGATTAAAG CTCGGCTCAG AGGGGAGAAG TATGACAGTG ATGGCTCAGA CAGTCAGGAG 
TCATGTGAAG AGGCAGTGCT CAGGATGAAG AAGATTGAGG CTTTGAAGGC CCAGTCAAAG 
GCTCGTGCTG CTGTACTGAA AGAGCAGCTG GAAAAGAAGA GAAGAGAAGC GTATGAAAGA 
GAGAAGAAAG CATGGGAGGA TCATCAGCTT GTCGCTCGAG GAGTAAAAGT TGCAGTTGGG 
CCTGTGGGTG GGACTCCCTC TCCTGCAGCC CCTCCAACTC CACCATCTGA CCCCACCCCT 
CAGGAAGCTC CTTCTAAAGC CACACCCTCC TCTAGACCCA GCACTCCAGC CATCTCTATG 
ACTGCTGCAC TGAAGGATGT GGGCGCGATT TCATCAGGGC AGAGTTCTCC TATGAAAGAG 
GACTCCCTTA AAGTTGAATC ATCAGATGTA CAGAGTGACA AGAAGGAGAT TCTTCGCAGA 
CTTAATCAGA AGGTTCAGAC CACTGAGGAA GTTTCTGTAA AAGAGCCCAC ACCCTCCAGC 
AATCAACCAT GTCCTGATCC TCAGCCAGTC CAACCCTCCA TCACAGAGGA AAGGCCTTCC 
ACAGCTGCAG ATAGGAAGAA GTGGGAAGCC GTAGCTCCAC CCATACTGTC TGTTGCTGAA 
CAGACTCTCG AGGAAACCTG TGCTACTCTG GCAGTGTCTC AGGTGGCATC ACCAGAAACG 
AATACTCCCT CGTGTGGAGA CAGGAAGAAA TGGCAGACAG ATGGTGTTCC TCTTCTGTCT 
GTGGCTCAGC AGACACTGGA AGAAACCAGC TTTAGAACAG CTGGTCAATT AACAGAAAAA 
ACAGTAGGTG AAGTCATTTG CATGGATGCC TGGCAGGGGG AGGGGCTAAG GAAGGCTTGG 
GGGCAGAGTC CAGATTCAGA GGTTTTGAAG GTGTTGGAAG AGGCAGAACT TCAGTCATTT 
ACTCAGAGAC TAGATCCTGC CAGCACGTGT GACCAAACAC TGACTGAAAG CCTTAATCCC 
ATTGAGGAAT CGGATGCCAC TAATGAAGCC AAGCAAGTGC CTCCTCCTGA ACCGTGCCAG 
CCTGCCACTG ACCATGACAA ACAAGAAGTG ACATGTCAAG AGACAGTGCC AATCATAGAA 
GAGGTTAAAA GTCAGGAACT TATCCAAACA ACAGCAGAGG GGACAGAAAA ATCTCATTCT 
GCAGAAAGTG ACATAGAGGT GGTGGATGAT ATGGAGTCAG TTGTTCTGGA TGATGTACCA 
CAGAAAACCG CAGAGCCGCC CCCTGCAGAA GTAATTCAGG CGTGGACTAA AGACACTCCA 
GAGTCTCTGC CAGCAGAGGG CAGAGTAATA CAAGCAGAAT GCACAGAGGT ACCTCAGAGA 
GAAGAGAAAC ATCCTGAACC TCCAGCAGAT GAGTCTTTGT TTGTGAAGTT AAGCAGTAGT 
CCAGCTCACA GACGCACAGT AGCGTTGGCC ATGCTGTCTG CTCAATCCTC ATTGGAAGAC 
TCTTCCTCTG CTGCCTCACG TTCTCGATCA GTGTCTCCAC TGCGCTCCCA CCGCCATGAA 
GATGCACTGC TCATCGGCCT CTCCACTGGC CTCTTCGATG CAAATAACCC AAAGATGCTA 
AGGACGTGCT CCTTACCAGA CCTCAGTAAG GTGTTTAGTG ATGCTACAGG ACCTGAAGGT 
GCAGCCGCTC CTGACAGCAA CCTAGAAATT GAAGATCTGG AAGATAAAGC AGATGAGCAG 
TCTGAACCTG AGGATGTGTA TGAGGATGAC GATTTAAGAG AGCTCAGAGC ATCTATGGAG 
AGGCTTCTTC AGGAGCAGCA CAGTGAAGAT GATGATGATG AAGAAGAGGA TGAGGGTGGT 
GGATCTAATG GCAGCCCGGC CGATGAAGAG CCAGCTGGTC TTAATGGGTT AGCTAGTGCT 
GGTGATGACA AACGCAGCCC AGAGGAAGAG ACTTTTAATG GAATGGCTGA AGATGATGAG 
GATGTTAGCC ATGGTGAAGA AGAACCAGGA GGCCCAGTCA CAAATGGAAT GGAATTTGAA 
GATGATGAAG AGCAGAACAG CAGTGAAGCA CAGCTCAATG AGGAGTGGCA GTCAGATGGC 
AGCGAGGAGG AGGACGACGA AGAGCTGGAA CAGCAGGACA GCATCTTCAG TCGTCTGGAG 
GAGCTGCGGC TTCATCTAGA GCAGGCCATG GGCTTTGAGA ACTTCATCCA GGCCTACAAC 
AAGATCAAGG CTATTCATGA GGATGAGGAT GAAAACATTG CAATGGGCTC CAGCATGGTG 
CAAAACATTT TGGGAACTGA GCACCAGCAC TTGTATCCTA AAATCCTCCA TTTGGTGATG 
GCAGATGGAG CTTACCAGGA AGATAATGAT GAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_009294681.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009292956.1 Danio rerio NIMA-related kinase 1 (nek1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ODa42286
Clone ID Related Accession (Same CDS sequence)	XM_009294681.3 , XM_009294681.2
Accession Version	XM_009294681.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4155bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase Nek1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009294681.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAAAT ATGAGAGGTT AAAGAAGATT GGGGAGGGAT CCTTTGGGAA AGCCATCTTG 
GTCAAGTCAA GGACAGATGG CCGACAATAT GTTATCAAGG AGATTGGTAT CTCCAGGATG 
TCGAACAAGG AACGACAGGA ATCACGTAAA GAGGTGGCAG TTCTGGCTAA CATGAGCCAT 
CCAAACATTG TCCAATACAA GGAATCCTTT GAAGAGAGTG GTTGTTTGTA CATTGTAATG 
GACTACTGTG AGGGAGGAGA CCTGTTTAAG AAGATCAATA ATCAAAGAGG ATCTCTTTTC 
CCAGAGGAAC AGATTCTTGA CTGGTTTGTG CAAATCTGTC TGGCTCTTAA ACATGTACAC 
GACCGCAAAA TCCTACATAG GGACATCAAA TCACAGAATA TTTTTCTGAC CAAAGATGGT 
ACTGTGCAGC TTGGAGACTT TGGAATCGCT AGAGTTTTGA ACAGCACCGT GGAGTTGGCA 
AGGACATGTA TCGGCACTCC TTATTACCTG TCGCCAGAGA TATGTGAAAA TAAACCATAC 
AACAACAAGA GTGATATCTG GGCCCTGGGA TGTGTCCTGT ATGAAATGTG CACGCTTAAG 
CATGCATTTG AAGCAGGAAA CATGAAGAAC CTGGTGCTGA AAATCATCCG TGGTTCATAC 
CCGCCTGTTT CCATCCATTA CTCCCCAGAT TTGCGCTCTC TCCTGGCCCA GCTCTTCAAG 
CGTAACCCCC GGGAGAGACC CTCCGTCTCG ACCATTTTGG ACAAGCCTTT TCTTGCTCGC 
AGAATTCACA AGTTTCTCAG CCCTCAGCTT ATTGCTCAGG AATTCAGCCA TTCAATTCAC 
CTTCAGCCCA AAATGAGTGT GGCCCATGCA GCACCAGCCA AACGTCCAGC CCCAGGCCCT 
ATCCCAATCA CCTCAGCTCA GAAAATCACC AAACCAGCAG CCAAATACGG AGTGCCTTTA 
ACAGTGCGGA GACCATCTGA AGTCGCCAGG AAGGTCCCAG ATGCAGCAAA GAAACCAGTT 
AAACTTAAGC AGGCTCCCCC TCCTGCAGCG GTTCAGAGGA GAGTGAGTCG AGTGGAGGAG 
GAGAGGAGAA AGCATGAGGA AGGAGTGAGG AAGAAGCGGA TGGAGATGAT GGAAAGAGAG 
AGAAAACAAA GAGAACAGAT GTTGTTACTG AAGGCTGCTC AGATGAAGAG ATTTGAGAGA 
GAAAAGCTGA ACCGAATCAA CCGAGCTCGA GAGCAGGGCT GGAGACACGT GCTGAGTTCT 
AGTGGAGGAA GCAGTCCTGA GCGGAAGTTT TATGGAGGTG GCGGCGGCAA TGGAGCTCCC 
CATTCCCCAG GAATATCTCC CTTTCCTGTG CCTGGACCCT CCCCTGTTCC TTCTTCAGCT 
TTAGCTCCAA GCAGAGCGCC ATATGACCGC ATCCACCCTT CAGCTGAAAA ATTAACCAAA 
CCTACACCTA AAGATGGTGC TGGTGAAGGA GCCGTGCATG CAGGGGACAA TGGCAGGACT 
TCTTCAGACT CAGGGACTCT AAATGGTCAT GCCTGCATTC CTGTTAAGAA TGGCGTCAGG 
AAAGAACAAC GTAAAACAAG TCTCCAAAAT GAGCAGATTT CAAAGATCGA CTATGCCAAT 
AGGTTTCGAG GTCAGCTGGC AGTAGAGAAA TCCAAGCAGG TGGAGGAGTT TCTACAGCGG 
AAAAGAGAAG CCATGCTGAA TAAAGTTCGT GCAGAAGGAC AGCTGGAGTA TTTGACCAGA 
TTACGACAGA TCAGACTACA GAACTTTAAT GAACGTCAGC AGATTAAAGC TCGGCTCAGA 
GGGGAGAAGT ATGACAGTGA TGGCTCAGAC AGTCAGGAGT CATGTGAAGA GGCAGTGCTC 
AGGATGAAGA AGATTGAGGC TTTGAAGGCC CAGTCAAAGG CTCGTGCTGC TGTACTGAAA 
GAGCAGCTGG AAAAGAAGAG AAGAGAAGCG TATGAAAGAG AGAAGAAAGC ATGGGAGGAT 
CATCAGCTTG TCGCTCGAGG AGTAAAAGTT GCAGTTGGGC CTGTGGGTGG GACTCCCTCT 
CCTGCAGCCC CTCCAACTCC ACCATCTGAC CCCACCCCTC AGGAAGCTCC TTCTAAAGCC 
ACACCCTCCT CTAGACCCAG CACTCCAGCC ATCTCTATGA CTGCTGCACT GAAGGATGTG 
GGCGCGATTT CATCAGGGCA GAGTTCTCCT ATGAAAGAGG ACTCCCTTAA AGTTGAATCA 
TCAGATGTAC AGAGTGACAA GAAGGAGATT CTTCGCAGAC TTAATCAGAA GGTTCAGACC 
ACTGAGGAAG TTTCTGTAAA AGAGCCCACA CCCTCCAGCA ATCAACCATG TCCTGATCCT 
CAGCCAGTCC AACCCTCCAT CACAGAGGAA AGGCCTTCCA CAGCTGCAGA TAGGAAGAAG 
TGGGAAGCCG TAGCTCCACC CATACTGTCT GTTGCTGAAC AGACTCTCGA GGAAACCTGT 
GCTACTCTGG CAGTGTCTCA GGTGGCATCA CCAGAAACGA ATACTCCCTC GTGTGGAGAC 
AGGAAGAAAT GGCAGACAGA TGGTGTTCCT CTTCTGTCTG TGGCTCAGCA GACACTGGAA 
GAAACCAGCT TTAGAACAGC TGGTCAATTA ACAGAAAAAA CAGTAGGTGA AGTCATTTGC 
ATGGATGCCT GGCAGGGGGA GGGGCTAAGG AAGGCTTGGG GGCAGAGTCC AGATTCAGAG 
GTTTTGAAGG TGTTGGAAGA GGCAGAACTT CAGTCATTTA CTCAGAGACT AGATCCTGCC 
AGCACGTGTG ACCAAACACT GACTGAAAGC CTTAATCCCA TTGAGGAATC GGATGCCACT 
AATGAAGCCA AGCAAGTGCC TCCTCCTGAA CCGTGCCAGC CTGCCACTGA CCATGACAAA 
CAAGAAGTGA CATGTCAAGA GACAGTGCCA ATCATAGAAG AGGTTAAAAG TCAGGAACTT 
ATCCAAACAA CAGCAGAGGG GACAGAAAAA TCTCATTCTG CAGAAAGTGA CATAGAGGTG 
GTGGATGATA TGGAGTCAGT TGTTCTGGAT GATGTACCAC AGAAAACCGC AGAGCCGCCC 
CCTGCAGAAG TAATTCAGGC GTGGACTAAA GACACTCCAG AGTCTCTGCC AGCAGAGGGC 
AGAGTAATAC AAGCAGAATG CACAGAGGTA CCTCAGAGAG AAGAGAAACA TCCTGAACCT 
CCAGCAGATG AGTCTTTGTT TGTGAAGTTA AGCAGTAGTC CAGCTCACAG ACGCACAGTA 
GCGTTGGCCA TGCTGTCTGC TCAATCCTCA TTGGAAGACT CTTCCTCTGC TGCCTCACGT 
TCTCGATCAG TGTCTCCACT GCGCTCCCAC CGCCATGAAG ATGCACTGCT CATCGGCCTC 
TCCACTGGCC TCTTCGATGC AAATAACCCA AAGATGCTAA GGACGTGCTC CTTACCAGAC 
CTCAGTAAGG TGTTTAGTGA TGCTACAGGA CCTGAAGGTG CAGCCGCTCC TGACAGCAAC 
CTAGAAATTG AAGATCTGGA AGATAAAGCA GATGAGCAGT CTGAACCTGA GGATGTGTAT 
GAGGATGACG ATTTAAGAGA GCTCAGAGCA TCTATGGAGA GGCTTCTTCA GGAGCAGCAC 
AGTGAAGATG ATGATGATGA AGAAGAGGAT GAGGGTGGTG GATCTAATGG CAGCCCGGCC 
GATGAAGAGC CAGCTGGTCT TAATGGGTTA GCTAGTGCTG GTGATGACAA ACGCAGCCCA 
GAGGAAGAGA CTTTTAATGG AATGGCTGAA GATGATGAGG ATGTTAGCCA TGGTGAAGAA 
GAACCAGGAG GCCCAGTCAC AAATGGAATG GAATTTGAAG ATGATGAAGA GCAGAACAGC 
AGTGAAGCAC AGCTCAATGA GGAGTGGCAG TCAGATGGCA GCGAGGAGGA GGACGACGAA 
GAGCTGGAAC AGCAGGACAG CATCTTCAGT CGTCTGGAGG AGCTGCGGCT TCATCTAGAG 
CAGGCCATGG GCTTTGAGAA CTTCATCCAG GCCTACAACA AGATCAAGGC TATTCATGAG 
GATGAGGATG AAAACATTGC AATGGGCTCC AGCATGGTGC AAAACATTTT GGGAACTGAG 
CACCAGCACT TGTATCCTAA AATCCTCCAT TTGGTGATGG CAGATGGAGC TTACCAGGAA 
GATAATGATG AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009292956.1
CDS	127..4281
Translation	MDKYERLKKIGEGSFGKAILVKSRTDGRQYVIKEIGISRMSNKERQESRKEVAVLANMSHPNIVQYKESFEESGCLYIVMDYCEGGDLFKKINNQRGSLFPEEQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIIRGSYPPVSIHYSPDLRSLLAQLFKRNPRERPSVSTILDKPFLARRIHKFLSPQLIAQEFSHSIHLQPKMSVAHAAPAKRPAPGPIPITSAQKITKPAAKYGVPLTVRRPSEVARKVPDAAKKPVKLKQAPPPAAVQRRVSRVEEERRKHEEGVRKKRMEMMERERKQREQMLLLKAAQMKRFEREKLNRINRAREQGWRHVLSSSGGSSPERKFYGGGGGNGAPHSPGISPFPVPGPSPVPSSALAPSRAPYDRIHPSAEKLTKPTPKDGAGEGAVHAGDNGRTSSDSGTLNGHACIPVKNGVRKEQRKTSLQNEQISKIDYANRFRGQLAVEKSKQVEEFLQRKREAMLNKVRAEGQLEYLTRLRQIRLQNFNERQQIKARLRGEKYDSDGSDSQESCEEAVLRMKKIEALKAQSKARAAVLKEQLEKKRREAYEREKKAWEDHQLVARGVKVAVGPVGGTPSPAAPPTPPSDPTPQEAPSKATPSSRPSTPAISMTAALKDVGAISSGQSSPMKEDSLKVESSDVQSDKKEILRRLNQKVQTTEEVSVKEPTPSSNQPCPDPQPVQPSITEERPSTAADRKKWEAVAPPILSVAEQTLEETCATLAVSQVASPETNTPSCGDRKKWQTDGVPLLSVAQQTLEETSFRTAGQLTEKTVGEVICMDAWQGEGLRKAWGQSPDSEVLKVLEEAELQSFTQRLDPASTCDQTLTESLNPIEESDATNEAKQVPPPEPCQPATDHDKQEVTCQETVPIIEEVKSQELIQTTAEGTEKSHSAESDIEVVDDMESVVLDDVPQKTAEPPPAEVIQAWTKDTPESLPAEGRVIQAECTEVPQREEKHPEPPADESLFVKLSSSPAHRRTVALAMLSAQSSLEDSSSAASRSRSVSPLRSHRHEDALLIGLSTGLFDANNPKMLRTCSLPDLSKVFSDATGPEGAAAPDSNLEIEDLEDKADEQSEPEDVYEDDDLRELRASMERLLQEQHSEDDDDEEEDEGGGSNGSPADEEPAGLNGLASAGDDKRSPEEETFNGMAEDDEDVSHGEEEPGGPVTNGMEFEDDEEQNSSEAQLNEEWQSDGSEEEDDEELEQQDSIFSRLEELRLHLEQAMGFENFIQAYNKIKAIHEDEDENIAMGSSMVQNILGTEHQHLYPKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_009294681.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio NIMA-related kinase 1 (nek1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009294681.3

ORF Insert Sequence:

ATGGATAAAT ATGAGAGGTT AAAGAAGATT GGGGAGGGAT CCTTTGGGAA AGCCATCTTG 
GTCAAGTCAA GGACAGATGG CCGACAATAT GTTATCAAGG AGATTGGTAT CTCCAGGATG 
TCGAACAAGG AACGACAGGA ATCACGTAAA GAGGTGGCAG TTCTGGCTAA CATGAGCCAT 
CCAAACATTG TCCAATACAA GGAATCCTTT GAAGAGAGTG GTTGTTTGTA CATTGTAATG 
GACTACTGTG AGGGAGGAGA CCTGTTTAAG AAGATCAATA ATCAAAGAGG ATCTCTTTTC 
CCAGAGGAAC AGATTCTTGA CTGGTTTGTG CAAATCTGTC TGGCTCTTAA ACATGTACAC 
GACCGCAAAA TCCTACATAG GGACATCAAA TCACAGAATA TTTTTCTGAC CAAAGATGGT 
ACTGTGCAGC TTGGAGACTT TGGAATCGCT AGAGTTTTGA ACAGCACCGT GGAGTTGGCA 
AGGACATGTA TCGGCACTCC TTATTACCTG TCGCCAGAGA TATGTGAAAA TAAACCATAC 
AACAACAAGA GTGATATCTG GGCCCTGGGA TGTGTCCTGT ATGAAATGTG CACGCTTAAG 
CATGCATTTG AAGCAGGAAA CATGAAGAAC CTGGTGCTGA AAATCATCCG TGGTTCATAC 
CCGCCTGTTT CCATCCATTA CTCCCCAGAT TTGCGCTCTC TCCTGGCCCA GCTCTTCAAG 
CGTAACCCCC GGGAGAGACC CTCCGTCTCG ACCATTTTGG ACAAGCCTTT TCTTGCTCGC 
AGAATTCACA AGTTTCTCAG CCCTCAGCTT ATTGCTCAGG AATTCAGCCA TTCAATTCAC 
CTTCAGCCCA AAATGAGTGT GGCCCATGCA GCACCAGCCA AACGTCCAGC CCCAGGCCCT 
ATCCCAATCA CCTCAGCTCA GAAAATCACC AAACCAGCAG CCAAATACGG AGTGCCTTTA 
ACAGTGCGGA GACCATCTGA AGTCGCCAGG AAGGTCCCAG ATGCAGCAAA GAAACCAGTT 
AAACTTAAGC AGGCTCCCCC TCCTGCAGCG GTTCAGAGGA GAGTGAGTCG AGTGGAGGAG 
GAGAGGAGAA AGCATGAGGA AGGAGTGAGG AAGAAGCGGA TGGAGATGAT GGAAAGAGAG 
AGAAAACAAA GAGAACAGAT GTTGTTACTG AAGGCTGCTC AGATGAAGAG ATTTGAGAGA 
GAAAAGCTGA ACCGAATCAA CCGAGCTCGA GAGCAGGGCT GGAGACACGT GCTGAGTTCT 
AGTGGAGGAA GCAGTCCTGA GCGGAAGTTT TATGGAGGTG GCGGCGGCAA TGGAGCTCCC 
CATTCCCCAG GAATATCTCC CTTTCCTGTG CCTGGACCCT CCCCTGTTCC TTCTTCAGCT 
TTAGCTCCAA GCAGAGCGCC ATATGACCGC ATCCACCCTT CAGCTGAAAA ATTAACCAAA 
CCTACACCTA AAGATGGTGC TGGTGAAGGA GCCGTGCATG CAGGGGACAA TGGCAGGACT 
TCTTCAGACT CAGGGACTCT AAATGGTCAT GCCTGCATTC CTGTTAAGAA TGGCGTCAGG 
AAAGAACAAC GTAAAACAAG TCTCCAAAAT GAGCAGATTT CAAAGATCGA CTATGCCAAT 
AGGTTTCGAG GTCAGCTGGC AGTAGAGAAA TCCAAGCAGG TGGAGGAGTT TCTACAGCGG 
AAAAGAGAAG CCATGCTGAA TAAAGTTCGT GCAGAAGGAC AGCTGGAGTA TTTGACCAGA 
TTACGACAGA TCAGACTACA GAACTTTAAT GAACGTCAGC AGATTAAAGC TCGGCTCAGA 
GGGGAGAAGT ATGACAGTGA TGGCTCAGAC AGTCAGGAGT CATGTGAAGA GGCAGTGCTC 
AGGATGAAGA AGATTGAGGC TTTGAAGGCC CAGTCAAAGG CTCGTGCTGC TGTACTGAAA 
GAGCAGCTGG AAAAGAAGAG AAGAGAAGCG TATGAAAGAG AGAAGAAAGC ATGGGAGGAT 
CATCAGCTTG TCGCTCGAGG AGTAAAAGTT GCAGTTGGGC CTGTGGGTGG GACTCCCTCT 
CCTGCAGCCC CTCCAACTCC ACCATCTGAC CCCACCCCTC AGGAAGCTCC TTCTAAAGCC 
ACACCCTCCT CTAGACCCAG CACTCCAGCC ATCTCTATGA CTGCTGCACT GAAGGATGTG 
GGCGCGATTT CATCAGGGCA GAGTTCTCCT ATGAAAGAGG ACTCCCTTAA AGTTGAATCA 
TCAGATGTAC AGAGTGACAA GAAGGAGATT CTTCGCAGAC TTAATCAGAA GGTTCAGACC 
ACTGAGGAAG TTTCTGTAAA AGAGCCCACA CCCTCCAGCA ATCAACCATG TCCTGATCCT 
CAGCCAGTCC AACCCTCCAT CACAGAGGAA AGGCCTTCCA CAGCTGCAGA TAGGAAGAAG 
TGGGAAGCCG TAGCTCCACC CATACTGTCT GTTGCTGAAC AGACTCTCGA GGAAACCTGT 
GCTACTCTGG CAGTGTCTCA GGTGGCATCA CCAGAAACGA ATACTCCCTC GTGTGGAGAC 
AGGAAGAAAT GGCAGACAGA TGGTGTTCCT CTTCTGTCTG TGGCTCAGCA GACACTGGAA 
GAAACCAGCT TTAGAACAGC TGGTCAATTA ACAGAAAAAA CAGTAGGTGA AGTCATTTGC 
ATGGATGCCT GGCAGGGGGA GGGGCTAAGG AAGGCTTGGG GGCAGAGTCC AGATTCAGAG 
GTTTTGAAGG TGTTGGAAGA GGCAGAACTT CAGTCATTTA CTCAGAGACT AGATCCTGCC 
AGCACGTGTG ACCAAACACT GACTGAAAGC CTTAATCCCA TTGAGGAATC GGATGCCACT 
AATGAAGCCA AGCAAGTGCC TCCTCCTGAA CCGTGCCAGC CTGCCACTGA CCATGACAAA 
CAAGAAGTGA CATGTCAAGA GACAGTGCCA ATCATAGAAG AGGTTAAAAG TCAGGAACTT 
ATCCAAACAA CAGCAGAGGG GACAGAAAAA TCTCATTCTG CAGAAAGTGA CATAGAGGTG 
GTGGATGATA TGGAGTCAGT TGTTCTGGAT GATGTACCAC AGAAAACCGC AGAGCCGCCC 
CCTGCAGAAG TAATTCAGGC GTGGACTAAA GACACTCCAG AGTCTCTGCC AGCAGAGGGC 
AGAGTAATAC AAGCAGAATG CACAGAGGTA CCTCAGAGAG AAGAGAAACA TCCTGAACCT 
CCAGCAGATG AGTCTTTGTT TGTGAAGTTA AGCAGTAGTC CAGCTCACAG ACGCACAGTA 
GCGTTGGCCA TGCTGTCTGC TCAATCCTCA TTGGAAGACT CTTCCTCTGC TGCCTCACGT 
TCTCGATCAG TGTCTCCACT GCGCTCCCAC CGCCATGAAG ATGCACTGCT CATCGGCCTC 
TCCACTGGCC TCTTCGATGC AAATAACCCA AAGATGCTAA GGACGTGCTC CTTACCAGAC 
CTCAGTAAGG TGTTTAGTGA TGCTACAGGA CCTGAAGGTG CAGCCGCTCC TGACAGCAAC 
CTAGAAATTG AAGATCTGGA AGATAAAGCA GATGAGCAGT CTGAACCTGA GGATGTGTAT 
GAGGATGACG ATTTAAGAGA GCTCAGAGCA TCTATGGAGA GGCTTCTTCA GGAGCAGCAC 
AGTGAAGATG ATGATGATGA AGAAGAGGAT GAGGGTGGTG GATCTAATGG CAGCCCGGCC 
GATGAAGAGC CAGCTGGTCT TAATGGGTTA GCTAGTGCTG GTGATGACAA ACGCAGCCCA 
GAGGAAGAGA CTTTTAATGG AATGGCTGAA GATGATGAGG ATGTTAGCCA TGGTGAAGAA 
GAACCAGGAG GCCCAGTCAC AAATGGAATG GAATTTGAAG ATGATGAAGA GCAGAACAGC 
AGTGAAGCAC AGCTCAATGA GGAGTGGCAG TCAGATGGCA GCGAGGAGGA GGACGACGAA 
GAGCTGGAAC AGCAGGACAG CATCTTCAGT CGTCTGGAGG AGCTGCGGCT TCATCTAGAG 
CAGGCCATGG GCTTTGAGAA CTTCATCCAG GCCTACAACA AGATCAAGGC TATTCATGAG 
GATGAGGATG AAAACATTGC AATGGGCTCC AGCATGGTGC AAAACATTTT GGGAACTGAG 
CACCAGCACT TGTATCCTAA AATCCTCCAT TTGGTGATGG CAGATGGAGC TTACCAGGAA 
GATAATGATG AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_009294681.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009292956.1 Danio rerio NIMA-related kinase 1 (nek1), transcript variant X5, mRNA.

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CloneID	ODa42286
Clone ID Related Accession (Same CDS sequence)	XM_009294681.3 , XM_009294681.2
Accession Version	XM_009294681.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4155bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	serine/threonine-protein kinase Nek1 isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009294681.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAAAT ATGAGAGGTT AAAGAAGATT GGGGAGGGAT CCTTTGGGAA AGCCATCTTG 
GTCAAGTCAA GGACAGATGG CCGACAATAT GTTATCAAGG AGATTGGTAT CTCCAGGATG 
TCGAACAAGG AACGACAGGA ATCACGTAAA GAGGTGGCAG TTCTGGCTAA CATGAGCCAT 
CCAAACATTG TCCAATACAA GGAATCCTTT GAAGAGAGTG GTTGTTTGTA CATTGTAATG 
GACTACTGTG AGGGAGGAGA CCTGTTTAAG AAGATCAATA ATCAAAGAGG ATCTCTTTTC 
CCAGAGGAAC AGATTCTTGA CTGGTTTGTG CAAATCTGTC TGGCTCTTAA ACATGTACAC 
GACCGCAAAA TCCTACATAG GGACATCAAA TCACAGAATA TTTTTCTGAC CAAAGATGGT 
ACTGTGCAGC TTGGAGACTT TGGAATCGCT AGAGTTTTGA ACAGCACCGT GGAGTTGGCA 
AGGACATGTA TCGGCACTCC TTATTACCTG TCGCCAGAGA TATGTGAAAA TAAACCATAC 
AACAACAAGA GTGATATCTG GGCCCTGGGA TGTGTCCTGT ATGAAATGTG CACGCTTAAG 
CATGCATTTG AAGCAGGAAA CATGAAGAAC CTGGTGCTGA AAATCATCCG TGGTTCATAC 
CCGCCTGTTT CCATCCATTA CTCCCCAGAT TTGCGCTCTC TCCTGGCCCA GCTCTTCAAG 
CGTAACCCCC GGGAGAGACC CTCCGTCTCG ACCATTTTGG ACAAGCCTTT TCTTGCTCGC 
AGAATTCACA AGTTTCTCAG CCCTCAGCTT ATTGCTCAGG AATTCAGCCA TTCAATTCAC 
CTTCAGCCCA AAATGAGTGT GGCCCATGCA GCACCAGCCA AACGTCCAGC CCCAGGCCCT 
ATCCCAATCA CCTCAGCTCA GAAAATCACC AAACCAGCAG CCAAATACGG AGTGCCTTTA 
ACAGTGCGGA GACCATCTGA AGTCGCCAGG AAGGTCCCAG ATGCAGCAAA GAAACCAGTT 
AAACTTAAGC AGGCTCCCCC TCCTGCAGCG GTTCAGAGGA GAGTGAGTCG AGTGGAGGAG 
GAGAGGAGAA AGCATGAGGA AGGAGTGAGG AAGAAGCGGA TGGAGATGAT GGAAAGAGAG 
AGAAAACAAA GAGAACAGAT GTTGTTACTG AAGGCTGCTC AGATGAAGAG ATTTGAGAGA 
GAAAAGCTGA ACCGAATCAA CCGAGCTCGA GAGCAGGGCT GGAGACACGT GCTGAGTTCT 
AGTGGAGGAA GCAGTCCTGA GCGGAAGTTT TATGGAGGTG GCGGCGGCAA TGGAGCTCCC 
CATTCCCCAG GAATATCTCC CTTTCCTGTG CCTGGACCCT CCCCTGTTCC TTCTTCAGCT 
TTAGCTCCAA GCAGAGCGCC ATATGACCGC ATCCACCCTT CAGCTGAAAA ATTAACCAAA 
CCTACACCTA AAGATGGTGC TGGTGAAGGA GCCGTGCATG CAGGGGACAA TGGCAGGACT 
TCTTCAGACT CAGGGACTCT AAATGGTCAT GCCTGCATTC CTGTTAAGAA TGGCGTCAGG 
AAAGAACAAC GTAAAACAAG TCTCCAAAAT GAGCAGATTT CAAAGATCGA CTATGCCAAT 
AGGTTTCGAG GTCAGCTGGC AGTAGAGAAA TCCAAGCAGG TGGAGGAGTT TCTACAGCGG 
AAAAGAGAAG CCATGCTGAA TAAAGTTCGT GCAGAAGGAC AGCTGGAGTA TTTGACCAGA 
TTACGACAGA TCAGACTACA GAACTTTAAT GAACGTCAGC AGATTAAAGC TCGGCTCAGA 
GGGGAGAAGT ATGACAGTGA TGGCTCAGAC AGTCAGGAGT CATGTGAAGA GGCAGTGCTC 
AGGATGAAGA AGATTGAGGC TTTGAAGGCC CAGTCAAAGG CTCGTGCTGC TGTACTGAAA 
GAGCAGCTGG AAAAGAAGAG AAGAGAAGCG TATGAAAGAG AGAAGAAAGC ATGGGAGGAT 
CATCAGCTTG TCGCTCGAGG AGTAAAAGTT GCAGTTGGGC CTGTGGGTGG GACTCCCTCT 
CCTGCAGCCC CTCCAACTCC ACCATCTGAC CCCACCCCTC AGGAAGCTCC TTCTAAAGCC 
ACACCCTCCT CTAGACCCAG CACTCCAGCC ATCTCTATGA CTGCTGCACT GAAGGATGTG 
GGCGCGATTT CATCAGGGCA GAGTTCTCCT ATGAAAGAGG ACTCCCTTAA AGTTGAATCA 
TCAGATGTAC AGAGTGACAA GAAGGAGATT CTTCGCAGAC TTAATCAGAA GGTTCAGACC 
ACTGAGGAAG TTTCTGTAAA AGAGCCCACA CCCTCCAGCA ATCAACCATG TCCTGATCCT 
CAGCCAGTCC AACCCTCCAT CACAGAGGAA AGGCCTTCCA CAGCTGCAGA TAGGAAGAAG 
TGGGAAGCCG TAGCTCCACC CATACTGTCT GTTGCTGAAC AGACTCTCGA GGAAACCTGT 
GCTACTCTGG CAGTGTCTCA GGTGGCATCA CCAGAAACGA ATACTCCCTC GTGTGGAGAC 
AGGAAGAAAT GGCAGACAGA TGGTGTTCCT CTTCTGTCTG TGGCTCAGCA GACACTGGAA 
GAAACCAGCT TTAGAACAGC TGGTCAATTA ACAGAAAAAA CAGTAGGTGA AGTCATTTGC 
ATGGATGCCT GGCAGGGGGA GGGGCTAAGG AAGGCTTGGG GGCAGAGTCC AGATTCAGAG 
GTTTTGAAGG TGTTGGAAGA GGCAGAACTT CAGTCATTTA CTCAGAGACT AGATCCTGCC 
AGCACGTGTG ACCAAACACT GACTGAAAGC CTTAATCCCA TTGAGGAATC GGATGCCACT 
AATGAAGCCA AGCAAGTGCC TCCTCCTGAA CCGTGCCAGC CTGCCACTGA CCATGACAAA 
CAAGAAGTGA CATGTCAAGA GACAGTGCCA ATCATAGAAG AGGTTAAAAG TCAGGAACTT 
ATCCAAACAA CAGCAGAGGG GACAGAAAAA TCTCATTCTG CAGAAAGTGA CATAGAGGTG 
GTGGATGATA TGGAGTCAGT TGTTCTGGAT GATGTACCAC AGAAAACCGC AGAGCCGCCC 
CCTGCAGAAG TAATTCAGGC GTGGACTAAA GACACTCCAG AGTCTCTGCC AGCAGAGGGC 
AGAGTAATAC AAGCAGAATG CACAGAGGTA CCTCAGAGAG AAGAGAAACA TCCTGAACCT 
CCAGCAGATG AGTCTTTGTT TGTGAAGTTA AGCAGTAGTC CAGCTCACAG ACGCACAGTA 
GCGTTGGCCA TGCTGTCTGC TCAATCCTCA TTGGAAGACT CTTCCTCTGC TGCCTCACGT 
TCTCGATCAG TGTCTCCACT GCGCTCCCAC CGCCATGAAG ATGCACTGCT CATCGGCCTC 
TCCACTGGCC TCTTCGATGC AAATAACCCA AAGATGCTAA GGACGTGCTC CTTACCAGAC 
CTCAGTAAGG TGTTTAGTGA TGCTACAGGA CCTGAAGGTG CAGCCGCTCC TGACAGCAAC 
CTAGAAATTG AAGATCTGGA AGATAAAGCA GATGAGCAGT CTGAACCTGA GGATGTGTAT 
GAGGATGACG ATTTAAGAGA GCTCAGAGCA TCTATGGAGA GGCTTCTTCA GGAGCAGCAC 
AGTGAAGATG ATGATGATGA AGAAGAGGAT GAGGGTGGTG GATCTAATGG CAGCCCGGCC 
GATGAAGAGC CAGCTGGTCT TAATGGGTTA GCTAGTGCTG GTGATGACAA ACGCAGCCCA 
GAGGAAGAGA CTTTTAATGG AATGGCTGAA GATGATGAGG ATGTTAGCCA TGGTGAAGAA 
GAACCAGGAG GCCCAGTCAC AAATGGAATG GAATTTGAAG ATGATGAAGA GCAGAACAGC 
AGTGAAGCAC AGCTCAATGA GGAGTGGCAG TCAGATGGCA GCGAGGAGGA GGACGACGAA 
GAGCTGGAAC AGCAGGACAG CATCTTCAGT CGTCTGGAGG AGCTGCGGCT TCATCTAGAG 
CAGGCCATGG GCTTTGAGAA CTTCATCCAG GCCTACAACA AGATCAAGGC TATTCATGAG 
GATGAGGATG AAAACATTGC AATGGGCTCC AGCATGGTGC AAAACATTTT GGGAACTGAG 
CACCAGCACT TGTATCCTAA AATCCTCCAT TTGGTGATGG CAGATGGAGC TTACCAGGAA 
GATAATGATG AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009292956.1
CDS	118..4272
Translation	MDKYERLKKIGEGSFGKAILVKSRTDGRQYVIKEIGISRMSNKERQESRKEVAVLANMSHPNIVQYKESFEESGCLYIVMDYCEGGDLFKKINNQRGSLFPEEQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIIRGSYPPVSIHYSPDLRSLLAQLFKRNPRERPSVSTILDKPFLARRIHKFLSPQLIAQEFSHSIHLQPKMSVAHAAPAKRPAPGPIPITSAQKITKPAAKYGVPLTVRRPSEVARKVPDAAKKPVKLKQAPPPAAVQRRVSRVEEERRKHEEGVRKKRMEMMERERKQREQMLLLKAAQMKRFEREKLNRINRAREQGWRHVLSSSGGSSPERKFYGGGGGNGAPHSPGISPFPVPGPSPVPSSALAPSRAPYDRIHPSAEKLTKPTPKDGAGEGAVHAGDNGRTSSDSGTLNGHACIPVKNGVRKEQRKTSLQNEQISKIDYANRFRGQLAVEKSKQVEEFLQRKREAMLNKVRAEGQLEYLTRLRQIRLQNFNERQQIKARLRGEKYDSDGSDSQESCEEAVLRMKKIEALKAQSKARAAVLKEQLEKKRREAYEREKKAWEDHQLVARGVKVAVGPVGGTPSPAAPPTPPSDPTPQEAPSKATPSSRPSTPAISMTAALKDVGAISSGQSSPMKEDSLKVESSDVQSDKKEILRRLNQKVQTTEEVSVKEPTPSSNQPCPDPQPVQPSITEERPSTAADRKKWEAVAPPILSVAEQTLEETCATLAVSQVASPETNTPSCGDRKKWQTDGVPLLSVAQQTLEETSFRTAGQLTEKTVGEVICMDAWQGEGLRKAWGQSPDSEVLKVLEEAELQSFTQRLDPASTCDQTLTESLNPIEESDATNEAKQVPPPEPCQPATDHDKQEVTCQETVPIIEEVKSQELIQTTAEGTEKSHSAESDIEVVDDMESVVLDDVPQKTAEPPPAEVIQAWTKDTPESLPAEGRVIQAECTEVPQREEKHPEPPADESLFVKLSSSPAHRRTVALAMLSAQSSLEDSSSAASRSRSVSPLRSHRHEDALLIGLSTGLFDANNPKMLRTCSLPDLSKVFSDATGPEGAAAPDSNLEIEDLEDKADEQSEPEDVYEDDDLRELRASMERLLQEQHSEDDDDEEEDEGGGSNGSPADEEPAGLNGLASAGDDKRSPEEETFNGMAEDDEDVSHGEEEPGGPVTNGMEFEDDEEQNSSEAQLNEEWQSDGSEEEDDEELEQQDSIFSRLEELRLHLEQAMGFENFIQAYNKIKAIHEDEDENIAMGSSMVQNILGTEHQHLYPKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_009294681.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio NIMA-related kinase 1 (nek1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009294681.2

ORF Insert Sequence:

ATGGATAAAT ATGAGAGGTT AAAGAAGATT GGGGAGGGAT CCTTTGGGAA AGCCATCTTG 
GTCAAGTCAA GGACAGATGG CCGACAATAT GTTATCAAGG AGATTGGTAT CTCCAGGATG 
TCGAACAAGG AACGACAGGA ATCACGTAAA GAGGTGGCAG TTCTGGCTAA CATGAGCCAT 
CCAAACATTG TCCAATACAA GGAATCCTTT GAAGAGAGTG GTTGTTTGTA CATTGTAATG 
GACTACTGTG AGGGAGGAGA CCTGTTTAAG AAGATCAATA ATCAAAGAGG ATCTCTTTTC 
CCAGAGGAAC AGATTCTTGA CTGGTTTGTG CAAATCTGTC TGGCTCTTAA ACATGTACAC 
GACCGCAAAA TCCTACATAG GGACATCAAA TCACAGAATA TTTTTCTGAC CAAAGATGGT 
ACTGTGCAGC TTGGAGACTT TGGAATCGCT AGAGTTTTGA ACAGCACCGT GGAGTTGGCA 
AGGACATGTA TCGGCACTCC TTATTACCTG TCGCCAGAGA TATGTGAAAA TAAACCATAC 
AACAACAAGA GTGATATCTG GGCCCTGGGA TGTGTCCTGT ATGAAATGTG CACGCTTAAG 
CATGCATTTG AAGCAGGAAA CATGAAGAAC CTGGTGCTGA AAATCATCCG TGGTTCATAC 
CCGCCTGTTT CCATCCATTA CTCCCCAGAT TTGCGCTCTC TCCTGGCCCA GCTCTTCAAG 
CGTAACCCCC GGGAGAGACC CTCCGTCTCG ACCATTTTGG ACAAGCCTTT TCTTGCTCGC 
AGAATTCACA AGTTTCTCAG CCCTCAGCTT ATTGCTCAGG AATTCAGCCA TTCAATTCAC 
CTTCAGCCCA AAATGAGTGT GGCCCATGCA GCACCAGCCA AACGTCCAGC CCCAGGCCCT 
ATCCCAATCA CCTCAGCTCA GAAAATCACC AAACCAGCAG CCAAATACGG AGTGCCTTTA 
ACAGTGCGGA GACCATCTGA AGTCGCCAGG AAGGTCCCAG ATGCAGCAAA GAAACCAGTT 
AAACTTAAGC AGGCTCCCCC TCCTGCAGCG GTTCAGAGGA GAGTGAGTCG AGTGGAGGAG 
GAGAGGAGAA AGCATGAGGA AGGAGTGAGG AAGAAGCGGA TGGAGATGAT GGAAAGAGAG 
AGAAAACAAA GAGAACAGAT GTTGTTACTG AAGGCTGCTC AGATGAAGAG ATTTGAGAGA 
GAAAAGCTGA ACCGAATCAA CCGAGCTCGA GAGCAGGGCT GGAGACACGT GCTGAGTTCT 
AGTGGAGGAA GCAGTCCTGA GCGGAAGTTT TATGGAGGTG GCGGCGGCAA TGGAGCTCCC 
CATTCCCCAG GAATATCTCC CTTTCCTGTG CCTGGACCCT CCCCTGTTCC TTCTTCAGCT 
TTAGCTCCAA GCAGAGCGCC ATATGACCGC ATCCACCCTT CAGCTGAAAA ATTAACCAAA 
CCTACACCTA AAGATGGTGC TGGTGAAGGA GCCGTGCATG CAGGGGACAA TGGCAGGACT 
TCTTCAGACT CAGGGACTCT AAATGGTCAT GCCTGCATTC CTGTTAAGAA TGGCGTCAGG 
AAAGAACAAC GTAAAACAAG TCTCCAAAAT GAGCAGATTT CAAAGATCGA CTATGCCAAT 
AGGTTTCGAG GTCAGCTGGC AGTAGAGAAA TCCAAGCAGG TGGAGGAGTT TCTACAGCGG 
AAAAGAGAAG CCATGCTGAA TAAAGTTCGT GCAGAAGGAC AGCTGGAGTA TTTGACCAGA 
TTACGACAGA TCAGACTACA GAACTTTAAT GAACGTCAGC AGATTAAAGC TCGGCTCAGA 
GGGGAGAAGT ATGACAGTGA TGGCTCAGAC AGTCAGGAGT CATGTGAAGA GGCAGTGCTC 
AGGATGAAGA AGATTGAGGC TTTGAAGGCC CAGTCAAAGG CTCGTGCTGC TGTACTGAAA 
GAGCAGCTGG AAAAGAAGAG AAGAGAAGCG TATGAAAGAG AGAAGAAAGC ATGGGAGGAT 
CATCAGCTTG TCGCTCGAGG AGTAAAAGTT GCAGTTGGGC CTGTGGGTGG GACTCCCTCT 
CCTGCAGCCC CTCCAACTCC ACCATCTGAC CCCACCCCTC AGGAAGCTCC TTCTAAAGCC 
ACACCCTCCT CTAGACCCAG CACTCCAGCC ATCTCTATGA CTGCTGCACT GAAGGATGTG 
GGCGCGATTT CATCAGGGCA GAGTTCTCCT ATGAAAGAGG ACTCCCTTAA AGTTGAATCA 
TCAGATGTAC AGAGTGACAA GAAGGAGATT CTTCGCAGAC TTAATCAGAA GGTTCAGACC 
ACTGAGGAAG TTTCTGTAAA AGAGCCCACA CCCTCCAGCA ATCAACCATG TCCTGATCCT 
CAGCCAGTCC AACCCTCCAT CACAGAGGAA AGGCCTTCCA CAGCTGCAGA TAGGAAGAAG 
TGGGAAGCCG TAGCTCCACC CATACTGTCT GTTGCTGAAC AGACTCTCGA GGAAACCTGT 
GCTACTCTGG CAGTGTCTCA GGTGGCATCA CCAGAAACGA ATACTCCCTC GTGTGGAGAC 
AGGAAGAAAT GGCAGACAGA TGGTGTTCCT CTTCTGTCTG TGGCTCAGCA GACACTGGAA 
GAAACCAGCT TTAGAACAGC TGGTCAATTA ACAGAAAAAA CAGTAGGTGA AGTCATTTGC 
ATGGATGCCT GGCAGGGGGA GGGGCTAAGG AAGGCTTGGG GGCAGAGTCC AGATTCAGAG 
GTTTTGAAGG TGTTGGAAGA GGCAGAACTT CAGTCATTTA CTCAGAGACT AGATCCTGCC 
AGCACGTGTG ACCAAACACT GACTGAAAGC CTTAATCCCA TTGAGGAATC GGATGCCACT 
AATGAAGCCA AGCAAGTGCC TCCTCCTGAA CCGTGCCAGC CTGCCACTGA CCATGACAAA 
CAAGAAGTGA CATGTCAAGA GACAGTGCCA ATCATAGAAG AGGTTAAAAG TCAGGAACTT 
ATCCAAACAA CAGCAGAGGG GACAGAAAAA TCTCATTCTG CAGAAAGTGA CATAGAGGTG 
GTGGATGATA TGGAGTCAGT TGTTCTGGAT GATGTACCAC AGAAAACCGC AGAGCCGCCC 
CCTGCAGAAG TAATTCAGGC GTGGACTAAA GACACTCCAG AGTCTCTGCC AGCAGAGGGC 
AGAGTAATAC AAGCAGAATG CACAGAGGTA CCTCAGAGAG AAGAGAAACA TCCTGAACCT 
CCAGCAGATG AGTCTTTGTT TGTGAAGTTA AGCAGTAGTC CAGCTCACAG ACGCACAGTA 
GCGTTGGCCA TGCTGTCTGC TCAATCCTCA TTGGAAGACT CTTCCTCTGC TGCCTCACGT 
TCTCGATCAG TGTCTCCACT GCGCTCCCAC CGCCATGAAG ATGCACTGCT CATCGGCCTC 
TCCACTGGCC TCTTCGATGC AAATAACCCA AAGATGCTAA GGACGTGCTC CTTACCAGAC 
CTCAGTAAGG TGTTTAGTGA TGCTACAGGA CCTGAAGGTG CAGCCGCTCC TGACAGCAAC 
CTAGAAATTG AAGATCTGGA AGATAAAGCA GATGAGCAGT CTGAACCTGA GGATGTGTAT 
GAGGATGACG ATTTAAGAGA GCTCAGAGCA TCTATGGAGA GGCTTCTTCA GGAGCAGCAC 
AGTGAAGATG ATGATGATGA AGAAGAGGAT GAGGGTGGTG GATCTAATGG CAGCCCGGCC 
GATGAAGAGC CAGCTGGTCT TAATGGGTTA GCTAGTGCTG GTGATGACAA ACGCAGCCCA 
GAGGAAGAGA CTTTTAATGG AATGGCTGAA GATGATGAGG ATGTTAGCCA TGGTGAAGAA 
GAACCAGGAG GCCCAGTCAC AAATGGAATG GAATTTGAAG ATGATGAAGA GCAGAACAGC 
AGTGAAGCAC AGCTCAATGA GGAGTGGCAG TCAGATGGCA GCGAGGAGGA GGACGACGAA 
GAGCTGGAAC AGCAGGACAG CATCTTCAGT CGTCTGGAGG AGCTGCGGCT TCATCTAGAG 
CAGGCCATGG GCTTTGAGAA CTTCATCCAG GCCTACAACA AGATCAAGGC TATTCATGAG 
GATGAGGATG AAAACATTGC AATGGGCTCC AGCATGGTGC AAAACATTTT GGGAACTGAG 
CACCAGCACT TGTATCCTAA AATCCTCCAT TTGGTGATGG CAGATGGAGC TTACCAGGAA 
GATAATGATG AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_009294682.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009292957.1 Danio rerio NIMA-related kinase 1 (nek1), transcript variant X6, mRNA.

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CloneID	ODa42287
Clone ID Related Accession (Same CDS sequence)	XM_009294682.2 , XM_009294682.3
Accession Version	XM_009294682.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4152bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	serine/threonine-protein kinase Nek1 isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009294682.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAAAT ATGAGAGGTT AAAGAAGATT GGGGAGGGAT CCTTTGGGAA AGCCATCTTG 
GTCAAGTCAA GGACAGATGG CCGACAATAT GTTATCAAGG AGATTGGTAT CTCCAGGATG 
TCGAACAAGG AACGACAGGA ATCACGTAAA GAGGTGGCAG TTCTGGCTAA CATGAGCCAT 
CCAAACATTG TCCAATACAA GGAATCCTTT GAAGAGAGTG GTTGTTTGTA CATTGTAATG 
GACTACTGTG AGGGAGGAGA CCTGTTTAAG AAGATCAATA ATCAAAGAGG ATCTCTTTTC 
CCAGAGGAAC AGATTCTTGA CTGGTTTGTG CAAATCTGTC TGGCTCTTAA ACATGTACAC 
GACCGCAAAA TCCTACATAG GGACATCAAA TCACAGAATA TTTTTCTGAC CAAAGATGGT 
ACTGTGCAGC TTGGAGACTT TGGAATCGCT AGAGTTTTGA ACAGCACCGT GGAGTTGGCA 
AGGACATGTA TCGGCACTCC TTATTACCTG TCGCCAGAGA TATGTGAAAA TAAACCATAC 
AACAACAAGA GTGATATCTG GGCCCTGGGA TGTGTCCTGT ATGAAATGTG CACGCTTAAG 
CATGCATTTG AAGCAGGAAA CATGAAGAAC CTGGTGCTGA AAATCATCCG TGGTTCATAC 
CCGCCTGTTT CCATCCATTA CTCCCCAGAT TTGCGCTCTC TCCTGGCCCA GCTCTTCAAG 
CGTAACCCCC GGGAGAGACC CTCCGTCTCG ACCATTTTGG ACAAGCCTTT TCTTGCTCGC 
AGAATTCACA AGTTTCTCAG CCCTCAGCTT ATTGCTCAGG AATTCAGCCA TTCAATTCAC 
CTTCAGCCCA AAATGAGTGT GGCCCATGCA GCACCAGCCA AACGTCCAGC CCCAGGCCCT 
ATCCCAATCA CCTCAGCTCA GAAAATCACC AAACCAGCAG CCAAATACGG AGTGCCTTTA 
ACAGTGCGGA GACCATCTGA AGTCGCCAGG AAGGTCCCAG ATGCAGCAAA GAAACCAGTT 
AAACTTAAGC AGGCTCCCCC TCCTGCAGCG GTTCAGAGGA GAGTGAGTCG AGTGGAGGAG 
GAGAGGAGAA AGCATGAGGA AGGAGTGAGG AAGAAGCGGA TGGAGATGAT GGAAAGAGAG 
AGAAAACAAA GAGAACAGAT GTTGTTACTG AAGGCTGCTC AGATGAAGAG ATTTGAGAGA 
GAAAAGCTGA ACCGAATCAA CCGAGCTCGA GAGCAGGGCT GGAGACACGT GCTGAGTTCT 
AGTGGAGGAA GCAGTCCTGA GCGGAAGTTT TATGGAGGTG GCGGCGGCAA TGGAGCTCCC 
CATTCCCCAG GAATATCTCC CTTTCCTGTG CCTGGACCCT CCCCTGTTCC TTCTTCAGCT 
TTAGCTCCAA GCAGAGCGCC ATATGACCGC ATCCACCCTT CAGCTGAAAA ATTAACCAAA 
CCTACACCTA AAGATGGTGC TGGTGAAGGA GCCGTGCATG CAGGGGACAA TGGCAGGACT 
TCTTCAGACT CAGGGACTCT AAATGGTCAT GCCTGCATTC CTGTTAAGAA TGGCGTCAGG 
AAAGAACAAC GTAAAACAAG TCTCCAAAAT GAGCAGATTT CAAAGATCGA CTATGCCAAT 
AGGTTTCGAG GTCAGCTGGC AGTAGAGAAA TCCAAGCAGG TGGAGGAGTT TCTACAGCGG 
AAAAGAGAAG CCATGCTGAA TAAAGTTCGT GCAGAAGGAC AGCTGGAGTA TTTGACCAGA 
TTACGACAGA TCAGACTACA GAACTTTAAT GAACGTCAGC AGATTAAAGC TCGGCTCAGA 
GGGGAGAAGT ATGACAGTGA TGGCTCAGAC AGTCAGGAGT CATGTGAAGA GGCAGTGCTC 
AGGATGAAGA AGATTGAGGC TTTGAAGGCC CAGTCAAAGG CTCGTGCTGC TGTACTGAAA 
GAGCAGCTGG AAAAGAAGAG AAGAGAAGCG TATGAAAGAG AGAAGAAAGC ATGGGAGGAT 
CATCTTGTCG CTCGAGGAGT AAAAGTTGCA GTTGGGCCTG TGGGTGGGAC TCCCTCTCCT 
GCAGCCCCTC CAACTCCACC ATCTGACCCC ACCCCTCAGG AAGCTCCTTC TAAAGCCACA 
CCCTCCTCTA GACCCAGCAC TCCAGCCATC TCTATGACTG CTGCACTGAA GGATGTGGGC 
GCGATTTCAT CAGGGCAGAG TTCTCCTATG AAAGAGGACT CCCTTAAAGT TGAATCATCA 
GATGTACAGA GTGACAAGAA GGAGATTCTT CGCAGACTTA ATCAGAAGGT TCAGACCACT 
GAGGAAGTTT CTGTAAAAGA GCCCACACCC TCCAGCAATC AACCATGTCC TGATCCTCAG 
CCAGTCCAAC CCTCCATCAC AGAGGAAAGG CCTTCCACAG CTGCAGATAG GAAGAAGTGG 
GAAGCCGTAG CTCCACCCAT ACTGTCTGTT GCTGAACAGA CTCTCGAGGA AACCTGTGCT 
ACTCTGGCAG TGTCTCAGGT GGCATCACCA GAAACGAATA CTCCCTCGTG TGGAGACAGG 
AAGAAATGGC AGACAGATGG TGTTCCTCTT CTGTCTGTGG CTCAGCAGAC ACTGGAAGAA 
ACCAGCTTTA GAACAGCTGG TCAATTAACA GAAAAAACAG TAGGTGAAGT CATTTGCATG 
GATGCCTGGC AGGGGGAGGG GCTAAGGAAG GCTTGGGGGC AGAGTCCAGA TTCAGAGGTT 
TTGAAGGTGT TGGAAGAGGC AGAACTTCAG TCATTTACTC AGAGACTAGA TCCTGCCAGC 
ACGTGTGACC AAACACTGAC TGAAAGCCTT AATCCCATTG AGGAATCGGA TGCCACTAAT 
GAAGCCAAGC AAGTGCCTCC TCCTGAACCG TGCCAGCCTG CCACTGACCA TGACAAACAA 
GAAGTGACAT GTCAAGAGAC AGTGCCAATC ATAGAAGAGG TTAAAAGTCA GGAACTTATC 
CAAACAACAG CAGAGGGGAC AGAAAAATCT CATTCTGCAG AAAGTGACAT AGAGGTGGTG 
GATGATATGG AGTCAGTTGT TCTGGATGAT GTACCACAGA AAACCGCAGA GCCGCCCCCT 
GCAGAAGTAA TTCAGGCGTG GACTAAAGAC ACTCCAGAGT CTCTGCCAGC AGAGGGCAGA 
GTAATACAAG CAGAATGCAC AGAGGTACCT CAGAGAGAAG AGAAACATCC TGAACCTCCA 
GCAGATGAGT CTTTGTTTGT GAAGTTAAGC AGTAGTCCAG CTCACAGACG CACAGTAGCG 
TTGGCCATGC TGTCTGCTCA ATCCTCATTG GAAGACTCTT CCTCTGCTGC CTCACGTTCT 
CGATCAGTGT CTCCACTGCG CTCCCACCGC CATGAAGATG CACTGCTCAT CGGCCTCTCC 
ACTGGCCTCT TCGATGCAAA TAACCCAAAG ATGCTAAGGA CGTGCTCCTT ACCAGACCTC 
AGTAAGGTGT TTAGTGATGC TACAGGACCT GAAGGTGCAG CCGCTCCTGA CAGCAACCTA 
GAAATTGAAG ATCTGGAAGA TAAAGCAGAT GAGCAGTCTG AACCTGAGGA TGTGTATGAG 
GATGACGATT TAAGAGAGCT CAGAGCATCT ATGGAGAGGC TTCTTCAGGA GCAGCACAGT 
GAAGATGATG ATGATGAAGA AGAGGATGAG GGTGGTGGAT CTAATGGCAG CCCGGCCGAT 
GAAGAGCCAG CTGGTCTTAA TGGGTTAGCT AGTGCTGGTG ATGACAAACG CAGCCCAGAG 
GAAGAGACTT TTAATGGAAT GGCTGAAGAT GATGAGGATG TTAGCCATGG TGAAGAAGAA 
CCAGGAGGCC CAGTCACAAA TGGAATGGAA TTTGAAGATG ATGAAGAGCA GAACAGCAGT 
GAAGCACAGC TCAATGAGGA GTGGCAGTCA GATGGCAGCG AGGAGGAGGA CGACGAAGAG 
CTGGAACAGC AGGACAGCAT CTTCAGTCGT CTGGAGGAGC TGCGGCTTCA TCTAGAGCAG 
GCCATGGGCT TTGAGAACTT CATCCAGGCC TACAACAAGA TCAAGGCTAT TCATGAGGAT 
GAGGATGAAA ACATTGCAAT GGGCTCCAGC ATGGTGCAAA ACATTTTGGG AACTGAGCAC 
CAGCACTTGT ATCCTAAAAT CCTCCATTTG GTGATGGCAG ATGGAGCTTA CCAGGAAGAT 
AATGATGAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009292957.1
CDS	118..4269
Translation	MDKYERLKKIGEGSFGKAILVKSRTDGRQYVIKEIGISRMSNKERQESRKEVAVLANMSHPNIVQYKESFEESGCLYIVMDYCEGGDLFKKINNQRGSLFPEEQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIIRGSYPPVSIHYSPDLRSLLAQLFKRNPRERPSVSTILDKPFLARRIHKFLSPQLIAQEFSHSIHLQPKMSVAHAAPAKRPAPGPIPITSAQKITKPAAKYGVPLTVRRPSEVARKVPDAAKKPVKLKQAPPPAAVQRRVSRVEEERRKHEEGVRKKRMEMMERERKQREQMLLLKAAQMKRFEREKLNRINRAREQGWRHVLSSSGGSSPERKFYGGGGGNGAPHSPGISPFPVPGPSPVPSSALAPSRAPYDRIHPSAEKLTKPTPKDGAGEGAVHAGDNGRTSSDSGTLNGHACIPVKNGVRKEQRKTSLQNEQISKIDYANRFRGQLAVEKSKQVEEFLQRKREAMLNKVRAEGQLEYLTRLRQIRLQNFNERQQIKARLRGEKYDSDGSDSQESCEEAVLRMKKIEALKAQSKARAAVLKEQLEKKRREAYEREKKAWEDHLVARGVKVAVGPVGGTPSPAAPPTPPSDPTPQEAPSKATPSSRPSTPAISMTAALKDVGAISSGQSSPMKEDSLKVESSDVQSDKKEILRRLNQKVQTTEEVSVKEPTPSSNQPCPDPQPVQPSITEERPSTAADRKKWEAVAPPILSVAEQTLEETCATLAVSQVASPETNTPSCGDRKKWQTDGVPLLSVAQQTLEETSFRTAGQLTEKTVGEVICMDAWQGEGLRKAWGQSPDSEVLKVLEEAELQSFTQRLDPASTCDQTLTESLNPIEESDATNEAKQVPPPEPCQPATDHDKQEVTCQETVPIIEEVKSQELIQTTAEGTEKSHSAESDIEVVDDMESVVLDDVPQKTAEPPPAEVIQAWTKDTPESLPAEGRVIQAECTEVPQREEKHPEPPADESLFVKLSSSPAHRRTVALAMLSAQSSLEDSSSAASRSRSVSPLRSHRHEDALLIGLSTGLFDANNPKMLRTCSLPDLSKVFSDATGPEGAAAPDSNLEIEDLEDKADEQSEPEDVYEDDDLRELRASMERLLQEQHSEDDDDEEEDEGGGSNGSPADEEPAGLNGLASAGDDKRSPEEETFNGMAEDDEDVSHGEEEPGGPVTNGMEFEDDEEQNSSEAQLNEEWQSDGSEEEDDEELEQQDSIFSRLEELRLHLEQAMGFENFIQAYNKIKAIHEDEDENIAMGSSMVQNILGTEHQHLYPKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_009294682.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio NIMA-related kinase 1 (nek1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009294682.2

ORF Insert Sequence:

ATGGATAAAT ATGAGAGGTT AAAGAAGATT GGGGAGGGAT CCTTTGGGAA AGCCATCTTG 
GTCAAGTCAA GGACAGATGG CCGACAATAT GTTATCAAGG AGATTGGTAT CTCCAGGATG 
TCGAACAAGG AACGACAGGA ATCACGTAAA GAGGTGGCAG TTCTGGCTAA CATGAGCCAT 
CCAAACATTG TCCAATACAA GGAATCCTTT GAAGAGAGTG GTTGTTTGTA CATTGTAATG 
GACTACTGTG AGGGAGGAGA CCTGTTTAAG AAGATCAATA ATCAAAGAGG ATCTCTTTTC 
CCAGAGGAAC AGATTCTTGA CTGGTTTGTG CAAATCTGTC TGGCTCTTAA ACATGTACAC 
GACCGCAAAA TCCTACATAG GGACATCAAA TCACAGAATA TTTTTCTGAC CAAAGATGGT 
ACTGTGCAGC TTGGAGACTT TGGAATCGCT AGAGTTTTGA ACAGCACCGT GGAGTTGGCA 
AGGACATGTA TCGGCACTCC TTATTACCTG TCGCCAGAGA TATGTGAAAA TAAACCATAC 
AACAACAAGA GTGATATCTG GGCCCTGGGA TGTGTCCTGT ATGAAATGTG CACGCTTAAG 
CATGCATTTG AAGCAGGAAA CATGAAGAAC CTGGTGCTGA AAATCATCCG TGGTTCATAC 
CCGCCTGTTT CCATCCATTA CTCCCCAGAT TTGCGCTCTC TCCTGGCCCA GCTCTTCAAG 
CGTAACCCCC GGGAGAGACC CTCCGTCTCG ACCATTTTGG ACAAGCCTTT TCTTGCTCGC 
AGAATTCACA AGTTTCTCAG CCCTCAGCTT ATTGCTCAGG AATTCAGCCA TTCAATTCAC 
CTTCAGCCCA AAATGAGTGT GGCCCATGCA GCACCAGCCA AACGTCCAGC CCCAGGCCCT 
ATCCCAATCA CCTCAGCTCA GAAAATCACC AAACCAGCAG CCAAATACGG AGTGCCTTTA 
ACAGTGCGGA GACCATCTGA AGTCGCCAGG AAGGTCCCAG ATGCAGCAAA GAAACCAGTT 
AAACTTAAGC AGGCTCCCCC TCCTGCAGCG GTTCAGAGGA GAGTGAGTCG AGTGGAGGAG 
GAGAGGAGAA AGCATGAGGA AGGAGTGAGG AAGAAGCGGA TGGAGATGAT GGAAAGAGAG 
AGAAAACAAA GAGAACAGAT GTTGTTACTG AAGGCTGCTC AGATGAAGAG ATTTGAGAGA 
GAAAAGCTGA ACCGAATCAA CCGAGCTCGA GAGCAGGGCT GGAGACACGT GCTGAGTTCT 
AGTGGAGGAA GCAGTCCTGA GCGGAAGTTT TATGGAGGTG GCGGCGGCAA TGGAGCTCCC 
CATTCCCCAG GAATATCTCC CTTTCCTGTG CCTGGACCCT CCCCTGTTCC TTCTTCAGCT 
TTAGCTCCAA GCAGAGCGCC ATATGACCGC ATCCACCCTT CAGCTGAAAA ATTAACCAAA 
CCTACACCTA AAGATGGTGC TGGTGAAGGA GCCGTGCATG CAGGGGACAA TGGCAGGACT 
TCTTCAGACT CAGGGACTCT AAATGGTCAT GCCTGCATTC CTGTTAAGAA TGGCGTCAGG 
AAAGAACAAC GTAAAACAAG TCTCCAAAAT GAGCAGATTT CAAAGATCGA CTATGCCAAT 
AGGTTTCGAG GTCAGCTGGC AGTAGAGAAA TCCAAGCAGG TGGAGGAGTT TCTACAGCGG 
AAAAGAGAAG CCATGCTGAA TAAAGTTCGT GCAGAAGGAC AGCTGGAGTA TTTGACCAGA 
TTACGACAGA TCAGACTACA GAACTTTAAT GAACGTCAGC AGATTAAAGC TCGGCTCAGA 
GGGGAGAAGT ATGACAGTGA TGGCTCAGAC AGTCAGGAGT CATGTGAAGA GGCAGTGCTC 
AGGATGAAGA AGATTGAGGC TTTGAAGGCC CAGTCAAAGG CTCGTGCTGC TGTACTGAAA 
GAGCAGCTGG AAAAGAAGAG AAGAGAAGCG TATGAAAGAG AGAAGAAAGC ATGGGAGGAT 
CATCTTGTCG CTCGAGGAGT AAAAGTTGCA GTTGGGCCTG TGGGTGGGAC TCCCTCTCCT 
GCAGCCCCTC CAACTCCACC ATCTGACCCC ACCCCTCAGG AAGCTCCTTC TAAAGCCACA 
CCCTCCTCTA GACCCAGCAC TCCAGCCATC TCTATGACTG CTGCACTGAA GGATGTGGGC 
GCGATTTCAT CAGGGCAGAG TTCTCCTATG AAAGAGGACT CCCTTAAAGT TGAATCATCA 
GATGTACAGA GTGACAAGAA GGAGATTCTT CGCAGACTTA ATCAGAAGGT TCAGACCACT 
GAGGAAGTTT CTGTAAAAGA GCCCACACCC TCCAGCAATC AACCATGTCC TGATCCTCAG 
CCAGTCCAAC CCTCCATCAC AGAGGAAAGG CCTTCCACAG CTGCAGATAG GAAGAAGTGG 
GAAGCCGTAG CTCCACCCAT ACTGTCTGTT GCTGAACAGA CTCTCGAGGA AACCTGTGCT 
ACTCTGGCAG TGTCTCAGGT GGCATCACCA GAAACGAATA CTCCCTCGTG TGGAGACAGG 
AAGAAATGGC AGACAGATGG TGTTCCTCTT CTGTCTGTGG CTCAGCAGAC ACTGGAAGAA 
ACCAGCTTTA GAACAGCTGG TCAATTAACA GAAAAAACAG TAGGTGAAGT CATTTGCATG 
GATGCCTGGC AGGGGGAGGG GCTAAGGAAG GCTTGGGGGC AGAGTCCAGA TTCAGAGGTT 
TTGAAGGTGT TGGAAGAGGC AGAACTTCAG TCATTTACTC AGAGACTAGA TCCTGCCAGC 
ACGTGTGACC AAACACTGAC TGAAAGCCTT AATCCCATTG AGGAATCGGA TGCCACTAAT 
GAAGCCAAGC AAGTGCCTCC TCCTGAACCG TGCCAGCCTG CCACTGACCA TGACAAACAA 
GAAGTGACAT GTCAAGAGAC AGTGCCAATC ATAGAAGAGG TTAAAAGTCA GGAACTTATC 
CAAACAACAG CAGAGGGGAC AGAAAAATCT CATTCTGCAG AAAGTGACAT AGAGGTGGTG 
GATGATATGG AGTCAGTTGT TCTGGATGAT GTACCACAGA AAACCGCAGA GCCGCCCCCT 
GCAGAAGTAA TTCAGGCGTG GACTAAAGAC ACTCCAGAGT CTCTGCCAGC AGAGGGCAGA 
GTAATACAAG CAGAATGCAC AGAGGTACCT CAGAGAGAAG AGAAACATCC TGAACCTCCA 
GCAGATGAGT CTTTGTTTGT GAAGTTAAGC AGTAGTCCAG CTCACAGACG CACAGTAGCG 
TTGGCCATGC TGTCTGCTCA ATCCTCATTG GAAGACTCTT CCTCTGCTGC CTCACGTTCT 
CGATCAGTGT CTCCACTGCG CTCCCACCGC CATGAAGATG CACTGCTCAT CGGCCTCTCC 
ACTGGCCTCT TCGATGCAAA TAACCCAAAG ATGCTAAGGA CGTGCTCCTT ACCAGACCTC 
AGTAAGGTGT TTAGTGATGC TACAGGACCT GAAGGTGCAG CCGCTCCTGA CAGCAACCTA 
GAAATTGAAG ATCTGGAAGA TAAAGCAGAT GAGCAGTCTG AACCTGAGGA TGTGTATGAG 
GATGACGATT TAAGAGAGCT CAGAGCATCT ATGGAGAGGC TTCTTCAGGA GCAGCACAGT 
GAAGATGATG ATGATGAAGA AGAGGATGAG GGTGGTGGAT CTAATGGCAG CCCGGCCGAT 
GAAGAGCCAG CTGGTCTTAA TGGGTTAGCT AGTGCTGGTG ATGACAAACG CAGCCCAGAG 
GAAGAGACTT TTAATGGAAT GGCTGAAGAT GATGAGGATG TTAGCCATGG TGAAGAAGAA 
CCAGGAGGCC CAGTCACAAA TGGAATGGAA TTTGAAGATG ATGAAGAGCA GAACAGCAGT 
GAAGCACAGC TCAATGAGGA GTGGCAGTCA GATGGCAGCG AGGAGGAGGA CGACGAAGAG 
CTGGAACAGC AGGACAGCAT CTTCAGTCGT CTGGAGGAGC TGCGGCTTCA TCTAGAGCAG 
GCCATGGGCT TTGAGAACTT CATCCAGGCC TACAACAAGA TCAAGGCTAT TCATGAGGAT 
GAGGATGAAA ACATTGCAAT GGGCTCCAGC ATGGTGCAAA ACATTTTGGG AACTGAGCAC 
CAGCACTTGT ATCCTAAAAT CCTCCATTTG GTGATGGCAG ATGGAGCTTA CCAGGAAGAT 
AATGATGAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_009294682.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009292957.1 Danio rerio NIMA-related kinase 1 (nek1), transcript variant X6, mRNA.

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CloneID	ODa42287
Clone ID Related Accession (Same CDS sequence)	XM_009294682.2 , XM_009294682.3
Accession Version	XM_009294682.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4152bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase Nek1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009294682.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAAAT ATGAGAGGTT AAAGAAGATT GGGGAGGGAT CCTTTGGGAA AGCCATCTTG 
GTCAAGTCAA GGACAGATGG CCGACAATAT GTTATCAAGG AGATTGGTAT CTCCAGGATG 
TCGAACAAGG AACGACAGGA ATCACGTAAA GAGGTGGCAG TTCTGGCTAA CATGAGCCAT 
CCAAACATTG TCCAATACAA GGAATCCTTT GAAGAGAGTG GTTGTTTGTA CATTGTAATG 
GACTACTGTG AGGGAGGAGA CCTGTTTAAG AAGATCAATA ATCAAAGAGG ATCTCTTTTC 
CCAGAGGAAC AGATTCTTGA CTGGTTTGTG CAAATCTGTC TGGCTCTTAA ACATGTACAC 
GACCGCAAAA TCCTACATAG GGACATCAAA TCACAGAATA TTTTTCTGAC CAAAGATGGT 
ACTGTGCAGC TTGGAGACTT TGGAATCGCT AGAGTTTTGA ACAGCACCGT GGAGTTGGCA 
AGGACATGTA TCGGCACTCC TTATTACCTG TCGCCAGAGA TATGTGAAAA TAAACCATAC 
AACAACAAGA GTGATATCTG GGCCCTGGGA TGTGTCCTGT ATGAAATGTG CACGCTTAAG 
CATGCATTTG AAGCAGGAAA CATGAAGAAC CTGGTGCTGA AAATCATCCG TGGTTCATAC 
CCGCCTGTTT CCATCCATTA CTCCCCAGAT TTGCGCTCTC TCCTGGCCCA GCTCTTCAAG 
CGTAACCCCC GGGAGAGACC CTCCGTCTCG ACCATTTTGG ACAAGCCTTT TCTTGCTCGC 
AGAATTCACA AGTTTCTCAG CCCTCAGCTT ATTGCTCAGG AATTCAGCCA TTCAATTCAC 
CTTCAGCCCA AAATGAGTGT GGCCCATGCA GCACCAGCCA AACGTCCAGC CCCAGGCCCT 
ATCCCAATCA CCTCAGCTCA GAAAATCACC AAACCAGCAG CCAAATACGG AGTGCCTTTA 
ACAGTGCGGA GACCATCTGA AGTCGCCAGG AAGGTCCCAG ATGCAGCAAA GAAACCAGTT 
AAACTTAAGC AGGCTCCCCC TCCTGCAGCG GTTCAGAGGA GAGTGAGTCG AGTGGAGGAG 
GAGAGGAGAA AGCATGAGGA AGGAGTGAGG AAGAAGCGGA TGGAGATGAT GGAAAGAGAG 
AGAAAACAAA GAGAACAGAT GTTGTTACTG AAGGCTGCTC AGATGAAGAG ATTTGAGAGA 
GAAAAGCTGA ACCGAATCAA CCGAGCTCGA GAGCAGGGCT GGAGACACGT GCTGAGTTCT 
AGTGGAGGAA GCAGTCCTGA GCGGAAGTTT TATGGAGGTG GCGGCGGCAA TGGAGCTCCC 
CATTCCCCAG GAATATCTCC CTTTCCTGTG CCTGGACCCT CCCCTGTTCC TTCTTCAGCT 
TTAGCTCCAA GCAGAGCGCC ATATGACCGC ATCCACCCTT CAGCTGAAAA ATTAACCAAA 
CCTACACCTA AAGATGGTGC TGGTGAAGGA GCCGTGCATG CAGGGGACAA TGGCAGGACT 
TCTTCAGACT CAGGGACTCT AAATGGTCAT GCCTGCATTC CTGTTAAGAA TGGCGTCAGG 
AAAGAACAAC GTAAAACAAG TCTCCAAAAT GAGCAGATTT CAAAGATCGA CTATGCCAAT 
AGGTTTCGAG GTCAGCTGGC AGTAGAGAAA TCCAAGCAGG TGGAGGAGTT TCTACAGCGG 
AAAAGAGAAG CCATGCTGAA TAAAGTTCGT GCAGAAGGAC AGCTGGAGTA TTTGACCAGA 
TTACGACAGA TCAGACTACA GAACTTTAAT GAACGTCAGC AGATTAAAGC TCGGCTCAGA 
GGGGAGAAGT ATGACAGTGA TGGCTCAGAC AGTCAGGAGT CATGTGAAGA GGCAGTGCTC 
AGGATGAAGA AGATTGAGGC TTTGAAGGCC CAGTCAAAGG CTCGTGCTGC TGTACTGAAA 
GAGCAGCTGG AAAAGAAGAG AAGAGAAGCG TATGAAAGAG AGAAGAAAGC ATGGGAGGAT 
CATCTTGTCG CTCGAGGAGT AAAAGTTGCA GTTGGGCCTG TGGGTGGGAC TCCCTCTCCT 
GCAGCCCCTC CAACTCCACC ATCTGACCCC ACCCCTCAGG AAGCTCCTTC TAAAGCCACA 
CCCTCCTCTA GACCCAGCAC TCCAGCCATC TCTATGACTG CTGCACTGAA GGATGTGGGC 
GCGATTTCAT CAGGGCAGAG TTCTCCTATG AAAGAGGACT CCCTTAAAGT TGAATCATCA 
GATGTACAGA GTGACAAGAA GGAGATTCTT CGCAGACTTA ATCAGAAGGT TCAGACCACT 
GAGGAAGTTT CTGTAAAAGA GCCCACACCC TCCAGCAATC AACCATGTCC TGATCCTCAG 
CCAGTCCAAC CCTCCATCAC AGAGGAAAGG CCTTCCACAG CTGCAGATAG GAAGAAGTGG 
GAAGCCGTAG CTCCACCCAT ACTGTCTGTT GCTGAACAGA CTCTCGAGGA AACCTGTGCT 
ACTCTGGCAG TGTCTCAGGT GGCATCACCA GAAACGAATA CTCCCTCGTG TGGAGACAGG 
AAGAAATGGC AGACAGATGG TGTTCCTCTT CTGTCTGTGG CTCAGCAGAC ACTGGAAGAA 
ACCAGCTTTA GAACAGCTGG TCAATTAACA GAAAAAACAG TAGGTGAAGT CATTTGCATG 
GATGCCTGGC AGGGGGAGGG GCTAAGGAAG GCTTGGGGGC AGAGTCCAGA TTCAGAGGTT 
TTGAAGGTGT TGGAAGAGGC AGAACTTCAG TCATTTACTC AGAGACTAGA TCCTGCCAGC 
ACGTGTGACC AAACACTGAC TGAAAGCCTT AATCCCATTG AGGAATCGGA TGCCACTAAT 
GAAGCCAAGC AAGTGCCTCC TCCTGAACCG TGCCAGCCTG CCACTGACCA TGACAAACAA 
GAAGTGACAT GTCAAGAGAC AGTGCCAATC ATAGAAGAGG TTAAAAGTCA GGAACTTATC 
CAAACAACAG CAGAGGGGAC AGAAAAATCT CATTCTGCAG AAAGTGACAT AGAGGTGGTG 
GATGATATGG AGTCAGTTGT TCTGGATGAT GTACCACAGA AAACCGCAGA GCCGCCCCCT 
GCAGAAGTAA TTCAGGCGTG GACTAAAGAC ACTCCAGAGT CTCTGCCAGC AGAGGGCAGA 
GTAATACAAG CAGAATGCAC AGAGGTACCT CAGAGAGAAG AGAAACATCC TGAACCTCCA 
GCAGATGAGT CTTTGTTTGT GAAGTTAAGC AGTAGTCCAG CTCACAGACG CACAGTAGCG 
TTGGCCATGC TGTCTGCTCA ATCCTCATTG GAAGACTCTT CCTCTGCTGC CTCACGTTCT 
CGATCAGTGT CTCCACTGCG CTCCCACCGC CATGAAGATG CACTGCTCAT CGGCCTCTCC 
ACTGGCCTCT TCGATGCAAA TAACCCAAAG ATGCTAAGGA CGTGCTCCTT ACCAGACCTC 
AGTAAGGTGT TTAGTGATGC TACAGGACCT GAAGGTGCAG CCGCTCCTGA CAGCAACCTA 
GAAATTGAAG ATCTGGAAGA TAAAGCAGAT GAGCAGTCTG AACCTGAGGA TGTGTATGAG 
GATGACGATT TAAGAGAGCT CAGAGCATCT ATGGAGAGGC TTCTTCAGGA GCAGCACAGT 
GAAGATGATG ATGATGAAGA AGAGGATGAG GGTGGTGGAT CTAATGGCAG CCCGGCCGAT 
GAAGAGCCAG CTGGTCTTAA TGGGTTAGCT AGTGCTGGTG ATGACAAACG CAGCCCAGAG 
GAAGAGACTT TTAATGGAAT GGCTGAAGAT GATGAGGATG TTAGCCATGG TGAAGAAGAA 
CCAGGAGGCC CAGTCACAAA TGGAATGGAA TTTGAAGATG ATGAAGAGCA GAACAGCAGT 
GAAGCACAGC TCAATGAGGA GTGGCAGTCA GATGGCAGCG AGGAGGAGGA CGACGAAGAG 
CTGGAACAGC AGGACAGCAT CTTCAGTCGT CTGGAGGAGC TGCGGCTTCA TCTAGAGCAG 
GCCATGGGCT TTGAGAACTT CATCCAGGCC TACAACAAGA TCAAGGCTAT TCATGAGGAT 
GAGGATGAAA ACATTGCAAT GGGCTCCAGC ATGGTGCAAA ACATTTTGGG AACTGAGCAC 
CAGCACTTGT ATCCTAAAAT CCTCCATTTG GTGATGGCAG ATGGAGCTTA CCAGGAAGAT 
AATGATGAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009292957.1
CDS	127..4278
Translation	MDKYERLKKIGEGSFGKAILVKSRTDGRQYVIKEIGISRMSNKERQESRKEVAVLANMSHPNIVQYKESFEESGCLYIVMDYCEGGDLFKKINNQRGSLFPEEQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIIRGSYPPVSIHYSPDLRSLLAQLFKRNPRERPSVSTILDKPFLARRIHKFLSPQLIAQEFSHSIHLQPKMSVAHAAPAKRPAPGPIPITSAQKITKPAAKYGVPLTVRRPSEVARKVPDAAKKPVKLKQAPPPAAVQRRVSRVEEERRKHEEGVRKKRMEMMERERKQREQMLLLKAAQMKRFEREKLNRINRAREQGWRHVLSSSGGSSPERKFYGGGGGNGAPHSPGISPFPVPGPSPVPSSALAPSRAPYDRIHPSAEKLTKPTPKDGAGEGAVHAGDNGRTSSDSGTLNGHACIPVKNGVRKEQRKTSLQNEQISKIDYANRFRGQLAVEKSKQVEEFLQRKREAMLNKVRAEGQLEYLTRLRQIRLQNFNERQQIKARLRGEKYDSDGSDSQESCEEAVLRMKKIEALKAQSKARAAVLKEQLEKKRREAYEREKKAWEDHLVARGVKVAVGPVGGTPSPAAPPTPPSDPTPQEAPSKATPSSRPSTPAISMTAALKDVGAISSGQSSPMKEDSLKVESSDVQSDKKEILRRLNQKVQTTEEVSVKEPTPSSNQPCPDPQPVQPSITEERPSTAADRKKWEAVAPPILSVAEQTLEETCATLAVSQVASPETNTPSCGDRKKWQTDGVPLLSVAQQTLEETSFRTAGQLTEKTVGEVICMDAWQGEGLRKAWGQSPDSEVLKVLEEAELQSFTQRLDPASTCDQTLTESLNPIEESDATNEAKQVPPPEPCQPATDHDKQEVTCQETVPIIEEVKSQELIQTTAEGTEKSHSAESDIEVVDDMESVVLDDVPQKTAEPPPAEVIQAWTKDTPESLPAEGRVIQAECTEVPQREEKHPEPPADESLFVKLSSSPAHRRTVALAMLSAQSSLEDSSSAASRSRSVSPLRSHRHEDALLIGLSTGLFDANNPKMLRTCSLPDLSKVFSDATGPEGAAAPDSNLEIEDLEDKADEQSEPEDVYEDDDLRELRASMERLLQEQHSEDDDDEEEDEGGGSNGSPADEEPAGLNGLASAGDDKRSPEEETFNGMAEDDEDVSHGEEEPGGPVTNGMEFEDDEEQNSSEAQLNEEWQSDGSEEEDDEELEQQDSIFSRLEELRLHLEQAMGFENFIQAYNKIKAIHEDEDENIAMGSSMVQNILGTEHQHLYPKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_009294682.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio NIMA-related kinase 1 (nek1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009294682.3

ORF Insert Sequence:

ATGGATAAAT ATGAGAGGTT AAAGAAGATT GGGGAGGGAT CCTTTGGGAA AGCCATCTTG 
GTCAAGTCAA GGACAGATGG CCGACAATAT GTTATCAAGG AGATTGGTAT CTCCAGGATG 
TCGAACAAGG AACGACAGGA ATCACGTAAA GAGGTGGCAG TTCTGGCTAA CATGAGCCAT 
CCAAACATTG TCCAATACAA GGAATCCTTT GAAGAGAGTG GTTGTTTGTA CATTGTAATG 
GACTACTGTG AGGGAGGAGA CCTGTTTAAG AAGATCAATA ATCAAAGAGG ATCTCTTTTC 
CCAGAGGAAC AGATTCTTGA CTGGTTTGTG CAAATCTGTC TGGCTCTTAA ACATGTACAC 
GACCGCAAAA TCCTACATAG GGACATCAAA TCACAGAATA TTTTTCTGAC CAAAGATGGT 
ACTGTGCAGC TTGGAGACTT TGGAATCGCT AGAGTTTTGA ACAGCACCGT GGAGTTGGCA 
AGGACATGTA TCGGCACTCC TTATTACCTG TCGCCAGAGA TATGTGAAAA TAAACCATAC 
AACAACAAGA GTGATATCTG GGCCCTGGGA TGTGTCCTGT ATGAAATGTG CACGCTTAAG 
CATGCATTTG AAGCAGGAAA CATGAAGAAC CTGGTGCTGA AAATCATCCG TGGTTCATAC 
CCGCCTGTTT CCATCCATTA CTCCCCAGAT TTGCGCTCTC TCCTGGCCCA GCTCTTCAAG 
CGTAACCCCC GGGAGAGACC CTCCGTCTCG ACCATTTTGG ACAAGCCTTT TCTTGCTCGC 
AGAATTCACA AGTTTCTCAG CCCTCAGCTT ATTGCTCAGG AATTCAGCCA TTCAATTCAC 
CTTCAGCCCA AAATGAGTGT GGCCCATGCA GCACCAGCCA AACGTCCAGC CCCAGGCCCT 
ATCCCAATCA CCTCAGCTCA GAAAATCACC AAACCAGCAG CCAAATACGG AGTGCCTTTA 
ACAGTGCGGA GACCATCTGA AGTCGCCAGG AAGGTCCCAG ATGCAGCAAA GAAACCAGTT 
AAACTTAAGC AGGCTCCCCC TCCTGCAGCG GTTCAGAGGA GAGTGAGTCG AGTGGAGGAG 
GAGAGGAGAA AGCATGAGGA AGGAGTGAGG AAGAAGCGGA TGGAGATGAT GGAAAGAGAG 
AGAAAACAAA GAGAACAGAT GTTGTTACTG AAGGCTGCTC AGATGAAGAG ATTTGAGAGA 
GAAAAGCTGA ACCGAATCAA CCGAGCTCGA GAGCAGGGCT GGAGACACGT GCTGAGTTCT 
AGTGGAGGAA GCAGTCCTGA GCGGAAGTTT TATGGAGGTG GCGGCGGCAA TGGAGCTCCC 
CATTCCCCAG GAATATCTCC CTTTCCTGTG CCTGGACCCT CCCCTGTTCC TTCTTCAGCT 
TTAGCTCCAA GCAGAGCGCC ATATGACCGC ATCCACCCTT CAGCTGAAAA ATTAACCAAA 
CCTACACCTA AAGATGGTGC TGGTGAAGGA GCCGTGCATG CAGGGGACAA TGGCAGGACT 
TCTTCAGACT CAGGGACTCT AAATGGTCAT GCCTGCATTC CTGTTAAGAA TGGCGTCAGG 
AAAGAACAAC GTAAAACAAG TCTCCAAAAT GAGCAGATTT CAAAGATCGA CTATGCCAAT 
AGGTTTCGAG GTCAGCTGGC AGTAGAGAAA TCCAAGCAGG TGGAGGAGTT TCTACAGCGG 
AAAAGAGAAG CCATGCTGAA TAAAGTTCGT GCAGAAGGAC AGCTGGAGTA TTTGACCAGA 
TTACGACAGA TCAGACTACA GAACTTTAAT GAACGTCAGC AGATTAAAGC TCGGCTCAGA 
GGGGAGAAGT ATGACAGTGA TGGCTCAGAC AGTCAGGAGT CATGTGAAGA GGCAGTGCTC 
AGGATGAAGA AGATTGAGGC TTTGAAGGCC CAGTCAAAGG CTCGTGCTGC TGTACTGAAA 
GAGCAGCTGG AAAAGAAGAG AAGAGAAGCG TATGAAAGAG AGAAGAAAGC ATGGGAGGAT 
CATCTTGTCG CTCGAGGAGT AAAAGTTGCA GTTGGGCCTG TGGGTGGGAC TCCCTCTCCT 
GCAGCCCCTC CAACTCCACC ATCTGACCCC ACCCCTCAGG AAGCTCCTTC TAAAGCCACA 
CCCTCCTCTA GACCCAGCAC TCCAGCCATC TCTATGACTG CTGCACTGAA GGATGTGGGC 
GCGATTTCAT CAGGGCAGAG TTCTCCTATG AAAGAGGACT CCCTTAAAGT TGAATCATCA 
GATGTACAGA GTGACAAGAA GGAGATTCTT CGCAGACTTA ATCAGAAGGT TCAGACCACT 
GAGGAAGTTT CTGTAAAAGA GCCCACACCC TCCAGCAATC AACCATGTCC TGATCCTCAG 
CCAGTCCAAC CCTCCATCAC AGAGGAAAGG CCTTCCACAG CTGCAGATAG GAAGAAGTGG 
GAAGCCGTAG CTCCACCCAT ACTGTCTGTT GCTGAACAGA CTCTCGAGGA AACCTGTGCT 
ACTCTGGCAG TGTCTCAGGT GGCATCACCA GAAACGAATA CTCCCTCGTG TGGAGACAGG 
AAGAAATGGC AGACAGATGG TGTTCCTCTT CTGTCTGTGG CTCAGCAGAC ACTGGAAGAA 
ACCAGCTTTA GAACAGCTGG TCAATTAACA GAAAAAACAG TAGGTGAAGT CATTTGCATG 
GATGCCTGGC AGGGGGAGGG GCTAAGGAAG GCTTGGGGGC AGAGTCCAGA TTCAGAGGTT 
TTGAAGGTGT TGGAAGAGGC AGAACTTCAG TCATTTACTC AGAGACTAGA TCCTGCCAGC 
ACGTGTGACC AAACACTGAC TGAAAGCCTT AATCCCATTG AGGAATCGGA TGCCACTAAT 
GAAGCCAAGC AAGTGCCTCC TCCTGAACCG TGCCAGCCTG CCACTGACCA TGACAAACAA 
GAAGTGACAT GTCAAGAGAC AGTGCCAATC ATAGAAGAGG TTAAAAGTCA GGAACTTATC 
CAAACAACAG CAGAGGGGAC AGAAAAATCT CATTCTGCAG AAAGTGACAT AGAGGTGGTG 
GATGATATGG AGTCAGTTGT TCTGGATGAT GTACCACAGA AAACCGCAGA GCCGCCCCCT 
GCAGAAGTAA TTCAGGCGTG GACTAAAGAC ACTCCAGAGT CTCTGCCAGC AGAGGGCAGA 
GTAATACAAG CAGAATGCAC AGAGGTACCT CAGAGAGAAG AGAAACATCC TGAACCTCCA 
GCAGATGAGT CTTTGTTTGT GAAGTTAAGC AGTAGTCCAG CTCACAGACG CACAGTAGCG 
TTGGCCATGC TGTCTGCTCA ATCCTCATTG GAAGACTCTT CCTCTGCTGC CTCACGTTCT 
CGATCAGTGT CTCCACTGCG CTCCCACCGC CATGAAGATG CACTGCTCAT CGGCCTCTCC 
ACTGGCCTCT TCGATGCAAA TAACCCAAAG ATGCTAAGGA CGTGCTCCTT ACCAGACCTC 
AGTAAGGTGT TTAGTGATGC TACAGGACCT GAAGGTGCAG CCGCTCCTGA CAGCAACCTA 
GAAATTGAAG ATCTGGAAGA TAAAGCAGAT GAGCAGTCTG AACCTGAGGA TGTGTATGAG 
GATGACGATT TAAGAGAGCT CAGAGCATCT ATGGAGAGGC TTCTTCAGGA GCAGCACAGT 
GAAGATGATG ATGATGAAGA AGAGGATGAG GGTGGTGGAT CTAATGGCAG CCCGGCCGAT 
GAAGAGCCAG CTGGTCTTAA TGGGTTAGCT AGTGCTGGTG ATGACAAACG CAGCCCAGAG 
GAAGAGACTT TTAATGGAAT GGCTGAAGAT GATGAGGATG TTAGCCATGG TGAAGAAGAA 
CCAGGAGGCC CAGTCACAAA TGGAATGGAA TTTGAAGATG ATGAAGAGCA GAACAGCAGT 
GAAGCACAGC TCAATGAGGA GTGGCAGTCA GATGGCAGCG AGGAGGAGGA CGACGAAGAG 
CTGGAACAGC AGGACAGCAT CTTCAGTCGT CTGGAGGAGC TGCGGCTTCA TCTAGAGCAG 
GCCATGGGCT TTGAGAACTT CATCCAGGCC TACAACAAGA TCAAGGCTAT TCATGAGGAT 
GAGGATGAAA ACATTGCAAT GGGCTCCAGC ATGGTGCAAA ACATTTTGGG AACTGAGCAC 
CAGCACTTGT ATCCTAAAAT CCTCCATTTG GTGATGGCAG ATGGAGCTTA CCAGGAAGAT 
AATGATGAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	NEK1
Entrez Gene ID	556002
Full Name	NIMA-related kinase 1
Synonyms	si:dkey-15j16.5,si:dkey-171k19.5,wu:fc38c08
General protein information	Preferred Names NIMA-related kinase 1 Names serine/threonine-protein kinase Nek1 NIMA (never in mitosis gene a)-related kinase 1
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

Bos taurus (cattle)	NEK1	XP_003586402.2
Rattus norvegicus (Norway rat)	Nek1	NP_001099552.2
Gallus gallus (chicken)	NEK1	XP_420401.3
Homo sapiens (human)	NEK1	NP_001186326.1
	NEK1	XP_001083275.1
Canis lupus familiaris (dog)	NEK1	XP_543184.2
Mus musculus (house mouse)	Nek1	NP_780298.2
Pan troglodytes (chimpanzee)	NEK1	XP_526727.2
Danio rerio (zebrafish)	nek1	XP_005160601.1
Xenopus tropicalis (tropical clawed frog)	nek1	NP_001090680.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

NEK1 cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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