NEK1 gene cDNA ORF clone, Macaca mulatta(Rhesus monkey)

mRNA	Protein	Name
XM_015139494.1	XP_014994980.1	serine/threonine-protein kinase Nek1 isoform X4
XM_015139493.1	XP_014994979.1	serine/threonine-protein kinase Nek1 isoform X3
XM_028848881.1	XP_028704714.1	serine/threonine-protein kinase Nek1 isoform X1
XM_015139492.2	XP_014994978.2	serine/threonine-protein kinase Nek1 isoform X5
XM_028848884.1	XP_028704717.1	serine/threonine-protein kinase Nek1 isoform X6
XM_001083275.3	XP_001083275.2	serine/threonine-protein kinase Nek1 isoform X1
XM_028848880.1	XP_028704713.1	serine/threonine-protein kinase Nek1 isoform X1
XM_028848883.1	XP_028704716.1	serine/threonine-protein kinase Nek1 isoform X4
XM_015139491.1	XP_014994977.1	serine/threonine-protein kinase Nek1 isoform X1
XM_015139492.1	XP_014994978.1	serine/threonine-protein kinase Nek1 isoform X2
XM_001083043.3	XP_001083043.2	serine/threonine-protein kinase Nek1 isoform X3
XM_028848882.1	XP_028704715.1	serine/threonine-protein kinase Nek1 isoform X2
XM_001083275.4	XP_001083275.3	serine/threonine-protein kinase Nek1 isoform X3
XM_028848879.1	XP_028704712.1	serine/threonine-protein kinase Nek1 isoform X1
XM_001083043.4	XP_001083043.3	serine/threonine-protein kinase Nek1 isoform X7
XM_015139494.2	XP_014994980.2	serine/threonine-protein kinase Nek1 isoform X8

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMb34267	XM_001083275.3 Latest version!	Macaca mulatta NIMA-related kinase 1 (NEK1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OMb34267	XM_015139491.1 Latest version!	Macaca mulatta NIMA-related kinase 1 (NEK1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OMb34268	XM_015139492.1 Latest version!	Macaca mulatta NIMA-related kinase 1 (NEK1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OMb34269	XM_015139493.1 Latest version!	Macaca mulatta NIMA-related kinase 1 (NEK1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OMb34269	XM_001083043.3 Latest version!	Macaca mulatta NIMA-related kinase 1 (NEK1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OMb34270	XM_015139494.1 Latest version!	Macaca mulatta NIMA-related kinase 1 (NEK1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OMb86669	XM_028848879.1 Latest version!	Macaca mulatta NIMA related kinase 1 (NEK1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMb86669	XM_028848881.1 Latest version!	Macaca mulatta NIMA related kinase 1 (NEK1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMb86669	XM_028848880.1 Latest version!	Macaca mulatta NIMA related kinase 1 (NEK1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMb86670	XM_028848882.1 Latest version!	Macaca mulatta NIMA related kinase 1 (NEK1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMb86671	XM_001083275.4 Latest version!	Macaca mulatta NIMA related kinase 1 (NEK1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMb86672	XM_028848883.1 Latest version!	Macaca mulatta NIMA related kinase 1 (NEK1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMb86673	XM_015139492.2 Latest version!	Macaca mulatta NIMA related kinase 1 (NEK1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMb86674	XM_028848884.1 Latest version!	Macaca mulatta NIMA related kinase 1 (NEK1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMb86675	XM_001083043.4 Latest version!	Macaca mulatta NIMA related kinase 1 (NEK1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMb86676	XM_015139494.2 Latest version!	Macaca mulatta NIMA related kinase 1 (NEK1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

NEK1 ( XM_001083275.3 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_001083275.2 Macaca mulatta NIMA-related kinase 1 (NEK1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMb34267
Clone ID Related Accession (Same CDS sequence)	XM_001083275.3 , XM_015139491.1
Accession Version	XM_001083275.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3861bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-20
Organism	Macaca mulatta(Rhesus monkey)
Product	serine/threonine-protein kinase Nek1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014804907.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 21, 2015 this sequence version replaced XM_001083275.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Macaca mulatta Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG CAGACAGTAT GTTATCAAGG AAATTAACAT CTCAAGAATG 
TCCAGTAAAG AAAGAGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GTTCTCTCTA CATAGTGATG 
GATTACTGTG AGGGAGGGGA TCTGTTTAAG CGAATAAATG CTCAGAAAGG CGTTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTGAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATTAAA TCTCAGAACA TATTTTTAAC TAAAGATGGA 
ACGGTACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AATAATAAAA GTGACATTTG GGCTCTGGGG TGTGTCCTTT ATGAGCTGTG TACACTTAAA 
CATGCTTTTG AAGCTGGCAG TATGAAAAAC TTGGTACTGA AGATAATATC CGGATCTTTT 
CCACCTGTGT CTTTGCATTA TTCCTATGAT CTCCGCAGTT TGGTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC ACCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACGTTT 
TCAAAGTTTG GATCACAGCC TATACCAGCC AAAAGACCAG CTTCAGGACA AAACTCGATT 
TCTGTTATGC CTGCTCAGAA AATTACAAAG CCTGCCGCTA AATATGGAAT ACCTTTAACA 
TATAAGAAAT ATGGAGAGAA AAAATTATAC GAAAAGAAAC CACTGCAAAA ACATAAACAG 
GCCCATCAAA CTCCAGAGAA GAGAGTGAAT ATTGGAGAAG AAAAGAGGAA AATGTCTGAG 
GAAGCAGCAA GAAAGAGAAG GCTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTATTAGTT TAATGAAGGC TGAACAAATG AAAAGGCAAG AAAAGGAAAG GTTGGAAAGA 
ATAAATAGGG CCAGGGAACA AGGATGGAGA AATGTGCTAA GTGCTGGTGG AGGTGGTGAA 
GTAAAGGCTC CTTTTCTTGG CAGTGGAGGG ACTATAGCTC CATCATCTTT TTCTTCTCGA 
GGACAGTATG AACATTACCA TGCCATTTTT GACCAAATGC AGCAACAGAG AGCAGAAGAT 
AATGAAGCTA AATGGAAAAG AGAAATATAT GGTCGAGGTC TTCCAGAAAG CGGAATTCTG 
CCTGGAGTTC GTCCAGGATT TCCTTATGGG GCTGCAGGTC ATCACCATTT CCCTGATGCT 
GATGATATTA GAAAAACTTT GAAAAGATTG AAGGCGGTGT CTAAACAAGC CAATGCAAAC 
AGGCAAAAAG GGCAGCTAGC TGTAGAAAGA GCTAAACAAG TAGAAGAGTT CCTGCAGCGA 
AAACGGGAAG CTATGCAGAA TAAAGCTCGA GCCGAAGGAC ATATGGGAAT CCTGCAAAAC 
CTGGCAGCTA TGTATGGAGG CAGGCCCAGC TCTTCAAGAG GAGGGAAGCC AAGGAACAAA 
GAGGAAGAGG TTTATCTGGC AAGACTGAGG CAAATAAGAC TACAGAATTT CAATGAACGC 
CAACAGATTA AAGCCAAACT TCGTGGTGAA AAGAAAGAAG CTAATCGTTC TGAAGGACAA 
GAAGGAAGTG AAGAGGCTGA CATGAGGCGC AAAAAAATCG AATCCCTGAA GGCCCATGCA 
AATGCACGTG CTGCTGTACT AAAAGAACAA CTAGAACGAA AGAGAAAGGA AGCTTATGAG 
AGAGAAAAAA AAGTGTGGGA AGAGCATTTG GTGGCTAAAG GAGTTAAGAG TTCTGATGTT 
TCTCCACCTT TGGGACAGCA TGAAACAGGT GGCTCTCCAT CAAAGCAACA GATGAGATCG 
GTTATTTCTG TGACTTCAGC TTTGAAAGAA GTTGGCGTGG ACAGTAGTTT AACTGATATC 
CGGGAAATTT CAGAAGAAAT GCAAAAGACC AACAATGCTA TTTCAAGTAA GCGAGAAATA 
CTTCGTAGAT TAAATGAAAA TCTTAAAGCT CAAGAAGATG AAAAAGGAAA GCAGCATCTC 
TCTGATACTT GTGAGATAAA TGTTCTTGAA GATGCCAAAG AGCATGAAAA AGAAAAATCA 
GTTTCATCTG ATCGCAAGAA GTGGGAGGCA GGAGGTCAAC TTGTGATTCC TCTGGATGAG 
TTAACACTAG ATACATCCTT CTCTACAACT GAAAGACATA CAGTGGGAGA AGTTATTAAA 
TTAGATCCTA ATGGATCTCC AAGAAGAGCC TGGGGGAAAA GTCCGACAGA TTCTGTTCTG 
AAGATACTTG GAGAAGCTGA ACTACAACTT CAGACAGAAC TATTAGAAAA TACAACTATT 
AGAAGTGATA TTTCTCCTGA AGGGGAAAAG TACAAACCCT TAATTACTGA AGAAAAAAAA 
GTGCAATGTA TTTCACATGA AATAAACCCA TCAGCTATTG TTGATTCTCC TGTTGAGACA 
GAAAGCCCCG AGTGTACTGA GGCATCTCCA CAGATGTCAT TGAAACTGGA AGGAAATTTA 
GAAGAACCTG ATGATTTGGA AACAGAAGTT CTACAAGAGC CAAGTGGAAC AAACAAAGAT 
GAGAGCTTGC CATGCACTGT TACTGATGTG TGGATTAGTG AGGAAAAAGA AGCAAAGGAA 
ACTCAGTTGG AAGATAGGAT CACCATTCAA GAAAATGAAG TTTCTGAGGA TGGAGTCTCG 
AGTACTGTGG ACCAACTTAG TGACATTCAT ATCGAGACGG GAGCCAATGA TTCTCAGCAC 
TCTAAATGTG ATGTAGATAA GTCTGTGCAA CCAGAGCCAT TTTTCCATAA AGTGGTTCAT 
TCTGAACACT TGAACTTAGT CCCTCAAGTT CAATCAGTTC AGTGTTCACC AGAAGAATCC 
TTTGCATTTC GATCTCACTC GCATTTACCA CCAAAAAATA AAAACAAGAA TTCCTTGCTG 
ATTGGACTTT CAACTGGTCT GTTTGATGCA AACAACCCAA AGATGTTAAG GACATGTTCA 
CTTCCAGATC TCTCAAAGCT GTTCAGAACC CTTATGGATG TTCCCACCGT AGGAGATGTT 
CGTCAAGACA ATCTTGAAAT AGATGAAATT GAAGATGAAA ACATTAAAGA AGGACCTTCT 
GATTCTGAAG ACATTGTGTT TGAAGAAACT GACACAGATT TACAAGAGCT GCAGGCCTCA 
ATGGAACAGT TACTTAGGGA ACAACCTAGT GAAGAATACA GTGAAGAAGA AGAGTCAGTC 
TTAAAGAACA GTGATGTGGA GCCAACTGCA AATGGGACAG ATGTGGCGGA TGAAGATGAC 
AATCCCAGCA GTGAAAGTGC CCTGAACGAA GAATGGCACT CAGATAACAG TGATGGGGAA 
AATGCTAGTG AATGTGAATA CGATAGTGTC TTTAACCATT TAGAGGAACT GAGACTTCAT 
CTGGAGCAGG AAATGGGCTT TGAAAAATTC TTTGAGGTTT ACGAGAAAAT AAAGGCTATT 
CATGAAGATG AAGATGAAAA TATTGAAATT TGTTCAAAAA TAGTTCAAAA TATTTTGGGA 
AATGAACATC AGCATCTTTA TGCCAAGATT CTTCATTTAG TCATGGCAGA TGGAGCCTAC 
CAAGAAGATA ATGATGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001083275.2
CDS	570..4430
Translation	MEKYVRLQKIGEGSFGKAILVKSTEDGRQYVIKEINISRMSSKEREESRREVAVLANMKHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGVLFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYELCTLKHAFEAGSMKNLVLKIISGSFPPVSLHYSYDLRSLVSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTFSKFGSQPIPAKRPASGQNSISVMPAQKITKPAAKYGIPLTYKKYGEKKLYEKKPLQKHKQAHQTPEKRVNIGEEKRKMSEEAARKRRLEFIEKEKKQKDQIISLMKAEQMKRQEKERLERINRAREQGWRNVLSAGGGGEVKAPFLGSGGTIAPSSFSSRGQYEHYHAIFDQMQQQRAEDNEAKWKREIYGRGLPESGILPGVRPGFPYGAAGHHHFPDADDIRKTLKRLKAVSKQANANRQKGQLAVERAKQVEEFLQRKREAMQNKARAEGHMGILQNLAAMYGGRPSSSRGGKPRNKEEEVYLARLRQIRLQNFNERQQIKAKLRGEKKEANRSEGQEGSEEADMRRKKIESLKAHANARAAVLKEQLERKRKEAYEREKKVWEEHLVAKGVKSSDVSPPLGQHETGGSPSKQQMRSVISVTSALKEVGVDSSLTDIREISEEMQKTNNAISSKREILRRLNENLKAQEDEKGKQHLSDTCEINVLEDAKEHEKEKSVSSDRKKWEAGGQLVIPLDELTLDTSFSTTERHTVGEVIKLDPNGSPRRAWGKSPTDSVLKILGEAELQLQTELLENTTIRSDISPEGEKYKPLITEEKKVQCISHEINPSAIVDSPVETESPECTEASPQMSLKLEGNLEEPDDLETEVLQEPSGTNKDESLPCTVTDVWISEEKEAKETQLEDRITIQENEVSEDGVSSTVDQLSDIHIETGANDSQHSKCDVDKSVQPEPFFHKVVHSEHLNLVPQVQSVQCSPEESFAFRSHSHLPPKNKNKNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDVRQDNLEIDEIEDENIKEGPSDSEDIVFEETDTDLQELQASMEQLLREQPSEEYSEEEESVLKNSDVEPTANGTDVADEDDNPSSESALNEEWHSDNSDGENASECEYDSVFNHLEELRLHLEQEMGFEKFFEVYEKIKAIHEDEDENIEICSKIVQNILGNEHQHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_001083275.3
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta NIMA-related kinase 1 (NEK1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001083275.3

ORF Insert Sequence:

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG CAGACAGTAT GTTATCAAGG AAATTAACAT CTCAAGAATG 
TCCAGTAAAG AAAGAGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GTTCTCTCTA CATAGTGATG 
GATTACTGTG AGGGAGGGGA TCTGTTTAAG CGAATAAATG CTCAGAAAGG CGTTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTGAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATTAAA TCTCAGAACA TATTTTTAAC TAAAGATGGA 
ACGGTACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AATAATAAAA GTGACATTTG GGCTCTGGGG TGTGTCCTTT ATGAGCTGTG TACACTTAAA 
CATGCTTTTG AAGCTGGCAG TATGAAAAAC TTGGTACTGA AGATAATATC CGGATCTTTT 
CCACCTGTGT CTTTGCATTA TTCCTATGAT CTCCGCAGTT TGGTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC ACCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACGTTT 
TCAAAGTTTG GATCACAGCC TATACCAGCC AAAAGACCAG CTTCAGGACA AAACTCGATT 
TCTGTTATGC CTGCTCAGAA AATTACAAAG CCTGCCGCTA AATATGGAAT ACCTTTAACA 
TATAAGAAAT ATGGAGAGAA AAAATTATAC GAAAAGAAAC CACTGCAAAA ACATAAACAG 
GCCCATCAAA CTCCAGAGAA GAGAGTGAAT ATTGGAGAAG AAAAGAGGAA AATGTCTGAG 
GAAGCAGCAA GAAAGAGAAG GCTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTATTAGTT TAATGAAGGC TGAACAAATG AAAAGGCAAG AAAAGGAAAG GTTGGAAAGA 
ATAAATAGGG CCAGGGAACA AGGATGGAGA AATGTGCTAA GTGCTGGTGG AGGTGGTGAA 
GTAAAGGCTC CTTTTCTTGG CAGTGGAGGG ACTATAGCTC CATCATCTTT TTCTTCTCGA 
GGACAGTATG AACATTACCA TGCCATTTTT GACCAAATGC AGCAACAGAG AGCAGAAGAT 
AATGAAGCTA AATGGAAAAG AGAAATATAT GGTCGAGGTC TTCCAGAAAG CGGAATTCTG 
CCTGGAGTTC GTCCAGGATT TCCTTATGGG GCTGCAGGTC ATCACCATTT CCCTGATGCT 
GATGATATTA GAAAAACTTT GAAAAGATTG AAGGCGGTGT CTAAACAAGC CAATGCAAAC 
AGGCAAAAAG GGCAGCTAGC TGTAGAAAGA GCTAAACAAG TAGAAGAGTT CCTGCAGCGA 
AAACGGGAAG CTATGCAGAA TAAAGCTCGA GCCGAAGGAC ATATGGGAAT CCTGCAAAAC 
CTGGCAGCTA TGTATGGAGG CAGGCCCAGC TCTTCAAGAG GAGGGAAGCC AAGGAACAAA 
GAGGAAGAGG TTTATCTGGC AAGACTGAGG CAAATAAGAC TACAGAATTT CAATGAACGC 
CAACAGATTA AAGCCAAACT TCGTGGTGAA AAGAAAGAAG CTAATCGTTC TGAAGGACAA 
GAAGGAAGTG AAGAGGCTGA CATGAGGCGC AAAAAAATCG AATCCCTGAA GGCCCATGCA 
AATGCACGTG CTGCTGTACT AAAAGAACAA CTAGAACGAA AGAGAAAGGA AGCTTATGAG 
AGAGAAAAAA AAGTGTGGGA AGAGCATTTG GTGGCTAAAG GAGTTAAGAG TTCTGATGTT 
TCTCCACCTT TGGGACAGCA TGAAACAGGT GGCTCTCCAT CAAAGCAACA GATGAGATCG 
GTTATTTCTG TGACTTCAGC TTTGAAAGAA GTTGGCGTGG ACAGTAGTTT AACTGATATC 
CGGGAAATTT CAGAAGAAAT GCAAAAGACC AACAATGCTA TTTCAAGTAA GCGAGAAATA 
CTTCGTAGAT TAAATGAAAA TCTTAAAGCT CAAGAAGATG AAAAAGGAAA GCAGCATCTC 
TCTGATACTT GTGAGATAAA TGTTCTTGAA GATGCCAAAG AGCATGAAAA AGAAAAATCA 
GTTTCATCTG ATCGCAAGAA GTGGGAGGCA GGAGGTCAAC TTGTGATTCC TCTGGATGAG 
TTAACACTAG ATACATCCTT CTCTACAACT GAAAGACATA CAGTGGGAGA AGTTATTAAA 
TTAGATCCTA ATGGATCTCC AAGAAGAGCC TGGGGGAAAA GTCCGACAGA TTCTGTTCTG 
AAGATACTTG GAGAAGCTGA ACTACAACTT CAGACAGAAC TATTAGAAAA TACAACTATT 
AGAAGTGATA TTTCTCCTGA AGGGGAAAAG TACAAACCCT TAATTACTGA AGAAAAAAAA 
GTGCAATGTA TTTCACATGA AATAAACCCA TCAGCTATTG TTGATTCTCC TGTTGAGACA 
GAAAGCCCCG AGTGTACTGA GGCATCTCCA CAGATGTCAT TGAAACTGGA AGGAAATTTA 
GAAGAACCTG ATGATTTGGA AACAGAAGTT CTACAAGAGC CAAGTGGAAC AAACAAAGAT 
GAGAGCTTGC CATGCACTGT TACTGATGTG TGGATTAGTG AGGAAAAAGA AGCAAAGGAA 
ACTCAGTTGG AAGATAGGAT CACCATTCAA GAAAATGAAG TTTCTGAGGA TGGAGTCTCG 
AGTACTGTGG ACCAACTTAG TGACATTCAT ATCGAGACGG GAGCCAATGA TTCTCAGCAC 
TCTAAATGTG ATGTAGATAA GTCTGTGCAA CCAGAGCCAT TTTTCCATAA AGTGGTTCAT 
TCTGAACACT TGAACTTAGT CCCTCAAGTT CAATCAGTTC AGTGTTCACC AGAAGAATCC 
TTTGCATTTC GATCTCACTC GCATTTACCA CCAAAAAATA AAAACAAGAA TTCCTTGCTG 
ATTGGACTTT CAACTGGTCT GTTTGATGCA AACAACCCAA AGATGTTAAG GACATGTTCA 
CTTCCAGATC TCTCAAAGCT GTTCAGAACC CTTATGGATG TTCCCACCGT AGGAGATGTT 
CGTCAAGACA ATCTTGAAAT AGATGAAATT GAAGATGAAA ACATTAAAGA AGGACCTTCT 
GATTCTGAAG ACATTGTGTT TGAAGAAACT GACACAGATT TACAAGAGCT GCAGGCCTCA 
ATGGAACAGT TACTTAGGGA ACAACCTAGT GAAGAATACA GTGAAGAAGA AGAGTCAGTC 
TTAAAGAACA GTGATGTGGA GCCAACTGCA AATGGGACAG ATGTGGCGGA TGAAGATGAC 
AATCCCAGCA GTGAAAGTGC CCTGAACGAA GAATGGCACT CAGATAACAG TGATGGGGAA 
AATGCTAGTG AATGTGAATA CGATAGTGTC TTTAACCATT TAGAGGAACT GAGACTTCAT 
CTGGAGCAGG AAATGGGCTT TGAAAAATTC TTTGAGGTTT ACGAGAAAAT AAAGGCTATT 
CATGAAGATG AAGATGAAAA TATTGAAATT TGTTCAAAAA TAGTTCAAAA TATTTTGGGA 
AATGAACATC AGCATCTTTA TGCCAAGATT CTTCATTTAG TCATGGCAGA TGGAGCCTAC 
CAAGAAGATA ATGATGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_015139491.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_014994977.1 Macaca mulatta NIMA-related kinase 1 (NEK1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMb34267
Clone ID Related Accession (Same CDS sequence)	XM_001083275.3 , XM_015139491.1
Accession Version	XM_015139491.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3861bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-20
Organism	Macaca mulatta(Rhesus monkey)
Product	serine/threonine-protein kinase Nek1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014804907.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Macaca mulatta Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG CAGACAGTAT GTTATCAAGG AAATTAACAT CTCAAGAATG 
TCCAGTAAAG AAAGAGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GTTCTCTCTA CATAGTGATG 
GATTACTGTG AGGGAGGGGA TCTGTTTAAG CGAATAAATG CTCAGAAAGG CGTTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTGAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATTAAA TCTCAGAACA TATTTTTAAC TAAAGATGGA 
ACGGTACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AATAATAAAA GTGACATTTG GGCTCTGGGG TGTGTCCTTT ATGAGCTGTG TACACTTAAA 
CATGCTTTTG AAGCTGGCAG TATGAAAAAC TTGGTACTGA AGATAATATC CGGATCTTTT 
CCACCTGTGT CTTTGCATTA TTCCTATGAT CTCCGCAGTT TGGTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC ACCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACGTTT 
TCAAAGTTTG GATCACAGCC TATACCAGCC AAAAGACCAG CTTCAGGACA AAACTCGATT 
TCTGTTATGC CTGCTCAGAA AATTACAAAG CCTGCCGCTA AATATGGAAT ACCTTTAACA 
TATAAGAAAT ATGGAGAGAA AAAATTATAC GAAAAGAAAC CACTGCAAAA ACATAAACAG 
GCCCATCAAA CTCCAGAGAA GAGAGTGAAT ATTGGAGAAG AAAAGAGGAA AATGTCTGAG 
GAAGCAGCAA GAAAGAGAAG GCTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTATTAGTT TAATGAAGGC TGAACAAATG AAAAGGCAAG AAAAGGAAAG GTTGGAAAGA 
ATAAATAGGG CCAGGGAACA AGGATGGAGA AATGTGCTAA GTGCTGGTGG AGGTGGTGAA 
GTAAAGGCTC CTTTTCTTGG CAGTGGAGGG ACTATAGCTC CATCATCTTT TTCTTCTCGA 
GGACAGTATG AACATTACCA TGCCATTTTT GACCAAATGC AGCAACAGAG AGCAGAAGAT 
AATGAAGCTA AATGGAAAAG AGAAATATAT GGTCGAGGTC TTCCAGAAAG CGGAATTCTG 
CCTGGAGTTC GTCCAGGATT TCCTTATGGG GCTGCAGGTC ATCACCATTT CCCTGATGCT 
GATGATATTA GAAAAACTTT GAAAAGATTG AAGGCGGTGT CTAAACAAGC CAATGCAAAC 
AGGCAAAAAG GGCAGCTAGC TGTAGAAAGA GCTAAACAAG TAGAAGAGTT CCTGCAGCGA 
AAACGGGAAG CTATGCAGAA TAAAGCTCGA GCCGAAGGAC ATATGGGAAT CCTGCAAAAC 
CTGGCAGCTA TGTATGGAGG CAGGCCCAGC TCTTCAAGAG GAGGGAAGCC AAGGAACAAA 
GAGGAAGAGG TTTATCTGGC AAGACTGAGG CAAATAAGAC TACAGAATTT CAATGAACGC 
CAACAGATTA AAGCCAAACT TCGTGGTGAA AAGAAAGAAG CTAATCGTTC TGAAGGACAA 
GAAGGAAGTG AAGAGGCTGA CATGAGGCGC AAAAAAATCG AATCCCTGAA GGCCCATGCA 
AATGCACGTG CTGCTGTACT AAAAGAACAA CTAGAACGAA AGAGAAAGGA AGCTTATGAG 
AGAGAAAAAA AAGTGTGGGA AGAGCATTTG GTGGCTAAAG GAGTTAAGAG TTCTGATGTT 
TCTCCACCTT TGGGACAGCA TGAAACAGGT GGCTCTCCAT CAAAGCAACA GATGAGATCG 
GTTATTTCTG TGACTTCAGC TTTGAAAGAA GTTGGCGTGG ACAGTAGTTT AACTGATATC 
CGGGAAATTT CAGAAGAAAT GCAAAAGACC AACAATGCTA TTTCAAGTAA GCGAGAAATA 
CTTCGTAGAT TAAATGAAAA TCTTAAAGCT CAAGAAGATG AAAAAGGAAA GCAGCATCTC 
TCTGATACTT GTGAGATAAA TGTTCTTGAA GATGCCAAAG AGCATGAAAA AGAAAAATCA 
GTTTCATCTG ATCGCAAGAA GTGGGAGGCA GGAGGTCAAC TTGTGATTCC TCTGGATGAG 
TTAACACTAG ATACATCCTT CTCTACAACT GAAAGACATA CAGTGGGAGA AGTTATTAAA 
TTAGATCCTA ATGGATCTCC AAGAAGAGCC TGGGGGAAAA GTCCGACAGA TTCTGTTCTG 
AAGATACTTG GAGAAGCTGA ACTACAACTT CAGACAGAAC TATTAGAAAA TACAACTATT 
AGAAGTGATA TTTCTCCTGA AGGGGAAAAG TACAAACCCT TAATTACTGA AGAAAAAAAA 
GTGCAATGTA TTTCACATGA AATAAACCCA TCAGCTATTG TTGATTCTCC TGTTGAGACA 
GAAAGCCCCG AGTGTACTGA GGCATCTCCA CAGATGTCAT TGAAACTGGA AGGAAATTTA 
GAAGAACCTG ATGATTTGGA AACAGAAGTT CTACAAGAGC CAAGTGGAAC AAACAAAGAT 
GAGAGCTTGC CATGCACTGT TACTGATGTG TGGATTAGTG AGGAAAAAGA AGCAAAGGAA 
ACTCAGTTGG AAGATAGGAT CACCATTCAA GAAAATGAAG TTTCTGAGGA TGGAGTCTCG 
AGTACTGTGG ACCAACTTAG TGACATTCAT ATCGAGACGG GAGCCAATGA TTCTCAGCAC 
TCTAAATGTG ATGTAGATAA GTCTGTGCAA CCAGAGCCAT TTTTCCATAA AGTGGTTCAT 
TCTGAACACT TGAACTTAGT CCCTCAAGTT CAATCAGTTC AGTGTTCACC AGAAGAATCC 
TTTGCATTTC GATCTCACTC GCATTTACCA CCAAAAAATA AAAACAAGAA TTCCTTGCTG 
ATTGGACTTT CAACTGGTCT GTTTGATGCA AACAACCCAA AGATGTTAAG GACATGTTCA 
CTTCCAGATC TCTCAAAGCT GTTCAGAACC CTTATGGATG TTCCCACCGT AGGAGATGTT 
CGTCAAGACA ATCTTGAAAT AGATGAAATT GAAGATGAAA ACATTAAAGA AGGACCTTCT 
GATTCTGAAG ACATTGTGTT TGAAGAAACT GACACAGATT TACAAGAGCT GCAGGCCTCA 
ATGGAACAGT TACTTAGGGA ACAACCTAGT GAAGAATACA GTGAAGAAGA AGAGTCAGTC 
TTAAAGAACA GTGATGTGGA GCCAACTGCA AATGGGACAG ATGTGGCGGA TGAAGATGAC 
AATCCCAGCA GTGAAAGTGC CCTGAACGAA GAATGGCACT CAGATAACAG TGATGGGGAA 
AATGCTAGTG AATGTGAATA CGATAGTGTC TTTAACCATT TAGAGGAACT GAGACTTCAT 
CTGGAGCAGG AAATGGGCTT TGAAAAATTC TTTGAGGTTT ACGAGAAAAT AAAGGCTATT 
CATGAAGATG AAGATGAAAA TATTGAAATT TGTTCAAAAA TAGTTCAAAA TATTTTGGGA 
AATGAACATC AGCATCTTTA TGCCAAGATT CTTCATTTAG TCATGGCAGA TGGAGCCTAC 
CAAGAAGATA ATGATGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014994977.1
CDS	264..4124
Translation	MEKYVRLQKIGEGSFGKAILVKSTEDGRQYVIKEINISRMSSKEREESRREVAVLANMKHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGVLFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYELCTLKHAFEAGSMKNLVLKIISGSFPPVSLHYSYDLRSLVSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTFSKFGSQPIPAKRPASGQNSISVMPAQKITKPAAKYGIPLTYKKYGEKKLYEKKPLQKHKQAHQTPEKRVNIGEEKRKMSEEAARKRRLEFIEKEKKQKDQIISLMKAEQMKRQEKERLERINRAREQGWRNVLSAGGGGEVKAPFLGSGGTIAPSSFSSRGQYEHYHAIFDQMQQQRAEDNEAKWKREIYGRGLPESGILPGVRPGFPYGAAGHHHFPDADDIRKTLKRLKAVSKQANANRQKGQLAVERAKQVEEFLQRKREAMQNKARAEGHMGILQNLAAMYGGRPSSSRGGKPRNKEEEVYLARLRQIRLQNFNERQQIKAKLRGEKKEANRSEGQEGSEEADMRRKKIESLKAHANARAAVLKEQLERKRKEAYEREKKVWEEHLVAKGVKSSDVSPPLGQHETGGSPSKQQMRSVISVTSALKEVGVDSSLTDIREISEEMQKTNNAISSKREILRRLNENLKAQEDEKGKQHLSDTCEINVLEDAKEHEKEKSVSSDRKKWEAGGQLVIPLDELTLDTSFSTTERHTVGEVIKLDPNGSPRRAWGKSPTDSVLKILGEAELQLQTELLENTTIRSDISPEGEKYKPLITEEKKVQCISHEINPSAIVDSPVETESPECTEASPQMSLKLEGNLEEPDDLETEVLQEPSGTNKDESLPCTVTDVWISEEKEAKETQLEDRITIQENEVSEDGVSSTVDQLSDIHIETGANDSQHSKCDVDKSVQPEPFFHKVVHSEHLNLVPQVQSVQCSPEESFAFRSHSHLPPKNKNKNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDVRQDNLEIDEIEDENIKEGPSDSEDIVFEETDTDLQELQASMEQLLREQPSEEYSEEEESVLKNSDVEPTANGTDVADEDDNPSSESALNEEWHSDNSDGENASECEYDSVFNHLEELRLHLEQEMGFEKFFEVYEKIKAIHEDEDENIEICSKIVQNILGNEHQHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_015139491.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta NIMA-related kinase 1 (NEK1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015139491.1

ORF Insert Sequence:

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG CAGACAGTAT GTTATCAAGG AAATTAACAT CTCAAGAATG 
TCCAGTAAAG AAAGAGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GTTCTCTCTA CATAGTGATG 
GATTACTGTG AGGGAGGGGA TCTGTTTAAG CGAATAAATG CTCAGAAAGG CGTTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTGAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATTAAA TCTCAGAACA TATTTTTAAC TAAAGATGGA 
ACGGTACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AATAATAAAA GTGACATTTG GGCTCTGGGG TGTGTCCTTT ATGAGCTGTG TACACTTAAA 
CATGCTTTTG AAGCTGGCAG TATGAAAAAC TTGGTACTGA AGATAATATC CGGATCTTTT 
CCACCTGTGT CTTTGCATTA TTCCTATGAT CTCCGCAGTT TGGTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC ACCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACGTTT 
TCAAAGTTTG GATCACAGCC TATACCAGCC AAAAGACCAG CTTCAGGACA AAACTCGATT 
TCTGTTATGC CTGCTCAGAA AATTACAAAG CCTGCCGCTA AATATGGAAT ACCTTTAACA 
TATAAGAAAT ATGGAGAGAA AAAATTATAC GAAAAGAAAC CACTGCAAAA ACATAAACAG 
GCCCATCAAA CTCCAGAGAA GAGAGTGAAT ATTGGAGAAG AAAAGAGGAA AATGTCTGAG 
GAAGCAGCAA GAAAGAGAAG GCTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTATTAGTT TAATGAAGGC TGAACAAATG AAAAGGCAAG AAAAGGAAAG GTTGGAAAGA 
ATAAATAGGG CCAGGGAACA AGGATGGAGA AATGTGCTAA GTGCTGGTGG AGGTGGTGAA 
GTAAAGGCTC CTTTTCTTGG CAGTGGAGGG ACTATAGCTC CATCATCTTT TTCTTCTCGA 
GGACAGTATG AACATTACCA TGCCATTTTT GACCAAATGC AGCAACAGAG AGCAGAAGAT 
AATGAAGCTA AATGGAAAAG AGAAATATAT GGTCGAGGTC TTCCAGAAAG CGGAATTCTG 
CCTGGAGTTC GTCCAGGATT TCCTTATGGG GCTGCAGGTC ATCACCATTT CCCTGATGCT 
GATGATATTA GAAAAACTTT GAAAAGATTG AAGGCGGTGT CTAAACAAGC CAATGCAAAC 
AGGCAAAAAG GGCAGCTAGC TGTAGAAAGA GCTAAACAAG TAGAAGAGTT CCTGCAGCGA 
AAACGGGAAG CTATGCAGAA TAAAGCTCGA GCCGAAGGAC ATATGGGAAT CCTGCAAAAC 
CTGGCAGCTA TGTATGGAGG CAGGCCCAGC TCTTCAAGAG GAGGGAAGCC AAGGAACAAA 
GAGGAAGAGG TTTATCTGGC AAGACTGAGG CAAATAAGAC TACAGAATTT CAATGAACGC 
CAACAGATTA AAGCCAAACT TCGTGGTGAA AAGAAAGAAG CTAATCGTTC TGAAGGACAA 
GAAGGAAGTG AAGAGGCTGA CATGAGGCGC AAAAAAATCG AATCCCTGAA GGCCCATGCA 
AATGCACGTG CTGCTGTACT AAAAGAACAA CTAGAACGAA AGAGAAAGGA AGCTTATGAG 
AGAGAAAAAA AAGTGTGGGA AGAGCATTTG GTGGCTAAAG GAGTTAAGAG TTCTGATGTT 
TCTCCACCTT TGGGACAGCA TGAAACAGGT GGCTCTCCAT CAAAGCAACA GATGAGATCG 
GTTATTTCTG TGACTTCAGC TTTGAAAGAA GTTGGCGTGG ACAGTAGTTT AACTGATATC 
CGGGAAATTT CAGAAGAAAT GCAAAAGACC AACAATGCTA TTTCAAGTAA GCGAGAAATA 
CTTCGTAGAT TAAATGAAAA TCTTAAAGCT CAAGAAGATG AAAAAGGAAA GCAGCATCTC 
TCTGATACTT GTGAGATAAA TGTTCTTGAA GATGCCAAAG AGCATGAAAA AGAAAAATCA 
GTTTCATCTG ATCGCAAGAA GTGGGAGGCA GGAGGTCAAC TTGTGATTCC TCTGGATGAG 
TTAACACTAG ATACATCCTT CTCTACAACT GAAAGACATA CAGTGGGAGA AGTTATTAAA 
TTAGATCCTA ATGGATCTCC AAGAAGAGCC TGGGGGAAAA GTCCGACAGA TTCTGTTCTG 
AAGATACTTG GAGAAGCTGA ACTACAACTT CAGACAGAAC TATTAGAAAA TACAACTATT 
AGAAGTGATA TTTCTCCTGA AGGGGAAAAG TACAAACCCT TAATTACTGA AGAAAAAAAA 
GTGCAATGTA TTTCACATGA AATAAACCCA TCAGCTATTG TTGATTCTCC TGTTGAGACA 
GAAAGCCCCG AGTGTACTGA GGCATCTCCA CAGATGTCAT TGAAACTGGA AGGAAATTTA 
GAAGAACCTG ATGATTTGGA AACAGAAGTT CTACAAGAGC CAAGTGGAAC AAACAAAGAT 
GAGAGCTTGC CATGCACTGT TACTGATGTG TGGATTAGTG AGGAAAAAGA AGCAAAGGAA 
ACTCAGTTGG AAGATAGGAT CACCATTCAA GAAAATGAAG TTTCTGAGGA TGGAGTCTCG 
AGTACTGTGG ACCAACTTAG TGACATTCAT ATCGAGACGG GAGCCAATGA TTCTCAGCAC 
TCTAAATGTG ATGTAGATAA GTCTGTGCAA CCAGAGCCAT TTTTCCATAA AGTGGTTCAT 
TCTGAACACT TGAACTTAGT CCCTCAAGTT CAATCAGTTC AGTGTTCACC AGAAGAATCC 
TTTGCATTTC GATCTCACTC GCATTTACCA CCAAAAAATA AAAACAAGAA TTCCTTGCTG 
ATTGGACTTT CAACTGGTCT GTTTGATGCA AACAACCCAA AGATGTTAAG GACATGTTCA 
CTTCCAGATC TCTCAAAGCT GTTCAGAACC CTTATGGATG TTCCCACCGT AGGAGATGTT 
CGTCAAGACA ATCTTGAAAT AGATGAAATT GAAGATGAAA ACATTAAAGA AGGACCTTCT 
GATTCTGAAG ACATTGTGTT TGAAGAAACT GACACAGATT TACAAGAGCT GCAGGCCTCA 
ATGGAACAGT TACTTAGGGA ACAACCTAGT GAAGAATACA GTGAAGAAGA AGAGTCAGTC 
TTAAAGAACA GTGATGTGGA GCCAACTGCA AATGGGACAG ATGTGGCGGA TGAAGATGAC 
AATCCCAGCA GTGAAAGTGC CCTGAACGAA GAATGGCACT CAGATAACAG TGATGGGGAA 
AATGCTAGTG AATGTGAATA CGATAGTGTC TTTAACCATT TAGAGGAACT GAGACTTCAT 
CTGGAGCAGG AAATGGGCTT TGAAAAATTC TTTGAGGTTT ACGAGAAAAT AAAGGCTATT 
CATGAAGATG AAGATGAAAA TATTGAAATT TGTTCAAAAA TAGTTCAAAA TATTTTGGGA 
AATGAACATC AGCATCTTTA TGCCAAGATT CTTCATTTAG TCATGGCAGA TGGAGCCTAC 
CAAGAAGATA ATGATGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_015139492.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_014994978.1 Macaca mulatta NIMA-related kinase 1 (NEK1), transcript variant X3, mRNA.

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CloneID	OMb34268
Clone ID Related Accession (Same CDS sequence)	XM_015139492.1
Accession Version	XM_015139492.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3777bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-20
Organism	Macaca mulatta(Rhesus monkey)
Product	serine/threonine-protein kinase Nek1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014804907.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Macaca mulatta Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG CAGACAGTAT GTTATCAAGG AAATTAACAT CTCAAGAATG 
TCCAGTAAAG AAAGAGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GTTCTCTCTA CATAGTGATG 
GATTACTGTG AGGGAGGGGA TCTGTTTAAG CGAATAAATG CTCAGAAAGG CGTTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTGAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATTAAA TCTCAGAACA TATTTTTAAC TAAAGATGGA 
ACGGTACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AATAATAAAA GTGACATTTG GGCTCTGGGG TGTGTCCTTT ATGAGCTGTG TACACTTAAA 
CATGCTTTTG AAGCTGGCAG TATGAAAAAC TTGGTACTGA AGATAATATC CGGATCTTTT 
CCACCTGTGT CTTTGCATTA TTCCTATGAT CTCCGCAGTT TGGTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC ACCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACGTTT 
TCAAAGTTTG GATCACAGCC TATACCAGCC AAAAGACCAG CTTCAGGACA AAACTCGATT 
TCTGTTATGC CTGCTCAGAA AATTACAAAG CCTGCCGCTA AATATGGAAT ACCTTTAACA 
TATAAGAAAT ATGGAGAGAA AAAATTATAC GAAAAGAAAC CACTGCAAAA ACATAAACAG 
GCCCATCAAA CTCCAGAGAA GAGAGTGAAT ATTGGAGAAG AAAAGAGGAA AATGTCTGAG 
GAAGCAGCAA GAAAGAGAAG GCTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTATTAGTT TAATGAAGGC TGAACAAATG AAAAGGCAAG AAAAGGAAAG GTTGGAAAGA 
ATAAATAGGG CCAGGGAACA AGGATGGAGA AATGTGCTAA GTGCTGGTGG AGGTGGTGAA 
GTAAAGGCTC CTTTTCTTGG CAGTGGAGGG ACTATAGCTC CATCATCTTT TTCTTCTCGA 
GGACAGTATG AACATTACCA TGCCATTTTT GACCAAATGC AGCAACAGAG AGCAGAAGAT 
AATGAAGCTA AATGGAAAAG AGAAATATAT GGTCGAGGTC TTCCAGAAAG CGGAATTCTG 
CCTGGAGTTC GTCCAGGATT TCCTTATGGG GCTGCAGGTC ATCACCATTT CCCTGATGCT 
GATGATATTA GAAAAACTTT GAAAAGATTG AAGGCGGTGT CTAAACAAGC CAATGCAAAC 
AGGCAAAAAG GGCAGCTAGC TGTAGAAAGA GCTAAACAAG TAGAAGAGTT CCTGCAGCGA 
AAACGGGAAG CTATGCAGAA TAAAGCTCGA GCCGAAGGAC ATATGGTTTA TCTGGCAAGA 
CTGAGGCAAA TAAGACTACA GAATTTCAAT GAACGCCAAC AGATTAAAGC CAAACTTCGT 
GGTGAAAAGA AAGAAGCTAA TCGTTCTGAA GGACAAGAAG GAAGTGAAGA GGCTGACATG 
AGGCGCAAAA AAATCGAATC CCTGAAGGCC CATGCAAATG CACGTGCTGC TGTACTAAAA 
GAACAACTAG AACGAAAGAG AAAGGAAGCT TATGAGAGAG AAAAAAAAGT GTGGGAAGAG 
CATTTGGTGG CTAAAGGAGT TAAGAGTTCT GATGTTTCTC CACCTTTGGG ACAGCATGAA 
ACAGGTGGCT CTCCATCAAA GCAACAGATG AGATCGGTTA TTTCTGTGAC TTCAGCTTTG 
AAAGAAGTTG GCGTGGACAG TAGTTTAACT GATATCCGGG AAATTTCAGA AGAAATGCAA 
AAGACCAACA ATGCTATTTC AAGTAAGCGA GAAATACTTC GTAGATTAAA TGAAAATCTT 
AAAGCTCAAG AAGATGAAAA AGGAAAGCAG CATCTCTCTG ATACTTGTGA GATAAATGTT 
CTTGAAGATG CCAAAGAGCA TGAAAAAGAA AAATCAGTTT CATCTGATCG CAAGAAGTGG 
GAGGCAGGAG GTCAACTTGT GATTCCTCTG GATGAGTTAA CACTAGATAC ATCCTTCTCT 
ACAACTGAAA GACATACAGT GGGAGAAGTT ATTAAATTAG ATCCTAATGG ATCTCCAAGA 
AGAGCCTGGG GGAAAAGTCC GACAGATTCT GTTCTGAAGA TACTTGGAGA AGCTGAACTA 
CAACTTCAGA CAGAACTATT AGAAAATACA ACTATTAGAA GTGATATTTC TCCTGAAGGG 
GAAAAGTACA AACCCTTAAT TACTGAAGAA AAAAAAGTGC AATGTATTTC ACATGAAATA 
AACCCATCAG CTATTGTTGA TTCTCCTGTT GAGACAGAAA GCCCCGAGTG TACTGAGGCA 
TCTCCACAGA TGTCATTGAA ACTGGAAGGA AATTTAGAAG AACCTGATGA TTTGGAAACA 
GAAGTTCTAC AAGAGCCAAG TGGAACAAAC AAAGATGAGA GCTTGCCATG CACTGTTACT 
GATGTGTGGA TTAGTGAGGA AAAAGAAGCA AAGGAAACTC AGTTGGAAGA TAGGATCACC 
ATTCAAGAAA ATGAAGTTTC TGAGGATGGA GTCTCGAGTA CTGTGGACCA ACTTAGTGAC 
ATTCATATCG AGACGGGAGC CAATGATTCT CAGCACTCTA AATGTGATGT AGATAAGTCT 
GTGCAACCAG AGCCATTTTT CCATAAAGTG GTTCATTCTG AACACTTGAA CTTAGTCCCT 
CAAGTTCAAT CAGTTCAGTG TTCACCAGAA GAATCCTTTG CATTTCGATC TCACTCGCAT 
TTACCACCAA AAAATAAAAA CAAGAATTCC TTGCTGATTG GACTTTCAAC TGGTCTGTTT 
GATGCAAACA ACCCAAAGAT GTTAAGGACA TGTTCACTTC CAGATCTCTC AAAGCTGTTC 
AGAACCCTTA TGGATGTTCC CACCGTAGGA GATGTTCGTC AAGACAATCT TGAAATAGAT 
GAAATTGAAG ATGAAAACAT TAAAGAAGGA CCTTCTGATT CTGAAGACAT TGTGTTTGAA 
GAAACTGACA CAGATTTACA AGAGCTGCAG GCCTCAATGG AACAGTTACT TAGGGAACAA 
CCTAGTGAAG AATACAGTGA AGAAGAAGAG TCAGTCTTAA AGAACAGTGA TGTGGAGCCA 
ACTGCAAATG GGACAGATGT GGCGGATGAA GATGACAATC CCAGCAGTGA AAGTGCCCTG 
AACGAAGAAT GGCACTCAGA TAACAGTGAT GGGGAAAATG CTAGTGAATG TGAATACGAT 
AGTGTCTTTA ACCATTTAGA GGAACTGAGA CTTCATCTGG AGCAGGAAAT GGGCTTTGAA 
AAATTCTTTG AGGTTTACGA GAAAATAAAG GCTATTCATG AAGATGAAGA TGAAAATATT 
GAAATTTGTT CAAAAATAGT TCAAAATATT TTGGGAAATG AACATCAGCA TCTTTATGCC 
AAGATTCTTC ATTTAGTCAT GGCAGATGGA GCCTACCAAG AAGATAATGA TGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014994978.1
CDS	645..4421
Translation	MEKYVRLQKIGEGSFGKAILVKSTEDGRQYVIKEINISRMSSKEREESRREVAVLANMKHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGVLFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYELCTLKHAFEAGSMKNLVLKIISGSFPPVSLHYSYDLRSLVSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTFSKFGSQPIPAKRPASGQNSISVMPAQKITKPAAKYGIPLTYKKYGEKKLYEKKPLQKHKQAHQTPEKRVNIGEEKRKMSEEAARKRRLEFIEKEKKQKDQIISLMKAEQMKRQEKERLERINRAREQGWRNVLSAGGGGEVKAPFLGSGGTIAPSSFSSRGQYEHYHAIFDQMQQQRAEDNEAKWKREIYGRGLPESGILPGVRPGFPYGAAGHHHFPDADDIRKTLKRLKAVSKQANANRQKGQLAVERAKQVEEFLQRKREAMQNKARAEGHMVYLARLRQIRLQNFNERQQIKAKLRGEKKEANRSEGQEGSEEADMRRKKIESLKAHANARAAVLKEQLERKRKEAYEREKKVWEEHLVAKGVKSSDVSPPLGQHETGGSPSKQQMRSVISVTSALKEVGVDSSLTDIREISEEMQKTNNAISSKREILRRLNENLKAQEDEKGKQHLSDTCEINVLEDAKEHEKEKSVSSDRKKWEAGGQLVIPLDELTLDTSFSTTERHTVGEVIKLDPNGSPRRAWGKSPTDSVLKILGEAELQLQTELLENTTIRSDISPEGEKYKPLITEEKKVQCISHEINPSAIVDSPVETESPECTEASPQMSLKLEGNLEEPDDLETEVLQEPSGTNKDESLPCTVTDVWISEEKEAKETQLEDRITIQENEVSEDGVSSTVDQLSDIHIETGANDSQHSKCDVDKSVQPEPFFHKVVHSEHLNLVPQVQSVQCSPEESFAFRSHSHLPPKNKNKNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDVRQDNLEIDEIEDENIKEGPSDSEDIVFEETDTDLQELQASMEQLLREQPSEEYSEEEESVLKNSDVEPTANGTDVADEDDNPSSESALNEEWHSDNSDGENASECEYDSVFNHLEELRLHLEQEMGFEKFFEVYEKIKAIHEDEDENIEICSKIVQNILGNEHQHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_015139492.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta NIMA-related kinase 1 (NEK1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015139492.1

ORF Insert Sequence:

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG CAGACAGTAT GTTATCAAGG AAATTAACAT CTCAAGAATG 
TCCAGTAAAG AAAGAGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GTTCTCTCTA CATAGTGATG 
GATTACTGTG AGGGAGGGGA TCTGTTTAAG CGAATAAATG CTCAGAAAGG CGTTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTGAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATTAAA TCTCAGAACA TATTTTTAAC TAAAGATGGA 
ACGGTACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AATAATAAAA GTGACATTTG GGCTCTGGGG TGTGTCCTTT ATGAGCTGTG TACACTTAAA 
CATGCTTTTG AAGCTGGCAG TATGAAAAAC TTGGTACTGA AGATAATATC CGGATCTTTT 
CCACCTGTGT CTTTGCATTA TTCCTATGAT CTCCGCAGTT TGGTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC ACCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACGTTT 
TCAAAGTTTG GATCACAGCC TATACCAGCC AAAAGACCAG CTTCAGGACA AAACTCGATT 
TCTGTTATGC CTGCTCAGAA AATTACAAAG CCTGCCGCTA AATATGGAAT ACCTTTAACA 
TATAAGAAAT ATGGAGAGAA AAAATTATAC GAAAAGAAAC CACTGCAAAA ACATAAACAG 
GCCCATCAAA CTCCAGAGAA GAGAGTGAAT ATTGGAGAAG AAAAGAGGAA AATGTCTGAG 
GAAGCAGCAA GAAAGAGAAG GCTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTATTAGTT TAATGAAGGC TGAACAAATG AAAAGGCAAG AAAAGGAAAG GTTGGAAAGA 
ATAAATAGGG CCAGGGAACA AGGATGGAGA AATGTGCTAA GTGCTGGTGG AGGTGGTGAA 
GTAAAGGCTC CTTTTCTTGG CAGTGGAGGG ACTATAGCTC CATCATCTTT TTCTTCTCGA 
GGACAGTATG AACATTACCA TGCCATTTTT GACCAAATGC AGCAACAGAG AGCAGAAGAT 
AATGAAGCTA AATGGAAAAG AGAAATATAT GGTCGAGGTC TTCCAGAAAG CGGAATTCTG 
CCTGGAGTTC GTCCAGGATT TCCTTATGGG GCTGCAGGTC ATCACCATTT CCCTGATGCT 
GATGATATTA GAAAAACTTT GAAAAGATTG AAGGCGGTGT CTAAACAAGC CAATGCAAAC 
AGGCAAAAAG GGCAGCTAGC TGTAGAAAGA GCTAAACAAG TAGAAGAGTT CCTGCAGCGA 
AAACGGGAAG CTATGCAGAA TAAAGCTCGA GCCGAAGGAC ATATGGTTTA TCTGGCAAGA 
CTGAGGCAAA TAAGACTACA GAATTTCAAT GAACGCCAAC AGATTAAAGC CAAACTTCGT 
GGTGAAAAGA AAGAAGCTAA TCGTTCTGAA GGACAAGAAG GAAGTGAAGA GGCTGACATG 
AGGCGCAAAA AAATCGAATC CCTGAAGGCC CATGCAAATG CACGTGCTGC TGTACTAAAA 
GAACAACTAG AACGAAAGAG AAAGGAAGCT TATGAGAGAG AAAAAAAAGT GTGGGAAGAG 
CATTTGGTGG CTAAAGGAGT TAAGAGTTCT GATGTTTCTC CACCTTTGGG ACAGCATGAA 
ACAGGTGGCT CTCCATCAAA GCAACAGATG AGATCGGTTA TTTCTGTGAC TTCAGCTTTG 
AAAGAAGTTG GCGTGGACAG TAGTTTAACT GATATCCGGG AAATTTCAGA AGAAATGCAA 
AAGACCAACA ATGCTATTTC AAGTAAGCGA GAAATACTTC GTAGATTAAA TGAAAATCTT 
AAAGCTCAAG AAGATGAAAA AGGAAAGCAG CATCTCTCTG ATACTTGTGA GATAAATGTT 
CTTGAAGATG CCAAAGAGCA TGAAAAAGAA AAATCAGTTT CATCTGATCG CAAGAAGTGG 
GAGGCAGGAG GTCAACTTGT GATTCCTCTG GATGAGTTAA CACTAGATAC ATCCTTCTCT 
ACAACTGAAA GACATACAGT GGGAGAAGTT ATTAAATTAG ATCCTAATGG ATCTCCAAGA 
AGAGCCTGGG GGAAAAGTCC GACAGATTCT GTTCTGAAGA TACTTGGAGA AGCTGAACTA 
CAACTTCAGA CAGAACTATT AGAAAATACA ACTATTAGAA GTGATATTTC TCCTGAAGGG 
GAAAAGTACA AACCCTTAAT TACTGAAGAA AAAAAAGTGC AATGTATTTC ACATGAAATA 
AACCCATCAG CTATTGTTGA TTCTCCTGTT GAGACAGAAA GCCCCGAGTG TACTGAGGCA 
TCTCCACAGA TGTCATTGAA ACTGGAAGGA AATTTAGAAG AACCTGATGA TTTGGAAACA 
GAAGTTCTAC AAGAGCCAAG TGGAACAAAC AAAGATGAGA GCTTGCCATG CACTGTTACT 
GATGTGTGGA TTAGTGAGGA AAAAGAAGCA AAGGAAACTC AGTTGGAAGA TAGGATCACC 
ATTCAAGAAA ATGAAGTTTC TGAGGATGGA GTCTCGAGTA CTGTGGACCA ACTTAGTGAC 
ATTCATATCG AGACGGGAGC CAATGATTCT CAGCACTCTA AATGTGATGT AGATAAGTCT 
GTGCAACCAG AGCCATTTTT CCATAAAGTG GTTCATTCTG AACACTTGAA CTTAGTCCCT 
CAAGTTCAAT CAGTTCAGTG TTCACCAGAA GAATCCTTTG CATTTCGATC TCACTCGCAT 
TTACCACCAA AAAATAAAAA CAAGAATTCC TTGCTGATTG GACTTTCAAC TGGTCTGTTT 
GATGCAAACA ACCCAAAGAT GTTAAGGACA TGTTCACTTC CAGATCTCTC AAAGCTGTTC 
AGAACCCTTA TGGATGTTCC CACCGTAGGA GATGTTCGTC AAGACAATCT TGAAATAGAT 
GAAATTGAAG ATGAAAACAT TAAAGAAGGA CCTTCTGATT CTGAAGACAT TGTGTTTGAA 
GAAACTGACA CAGATTTACA AGAGCTGCAG GCCTCAATGG AACAGTTACT TAGGGAACAA 
CCTAGTGAAG AATACAGTGA AGAAGAAGAG TCAGTCTTAA AGAACAGTGA TGTGGAGCCA 
ACTGCAAATG GGACAGATGT GGCGGATGAA GATGACAATC CCAGCAGTGA AAGTGCCCTG 
AACGAAGAAT GGCACTCAGA TAACAGTGAT GGGGAAAATG CTAGTGAATG TGAATACGAT 
AGTGTCTTTA ACCATTTAGA GGAACTGAGA CTTCATCTGG AGCAGGAAAT GGGCTTTGAA 
AAATTCTTTG AGGTTTACGA GAAAATAAAG GCTATTCATG AAGATGAAGA TGAAAATATT 
GAAATTTGTT CAAAAATAGT TCAAAATATT TTGGGAAATG AACATCAGCA TCTTTATGCC 
AAGATTCTTC ATTTAGTCAT GGCAGATGGA GCCTACCAAG AAGATAATGA TGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_015139493.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_014994979.1 Macaca mulatta NIMA-related kinase 1 (NEK1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMb34269
Clone ID Related Accession (Same CDS sequence)	XM_015139493.1 , XM_001083043.3
Accession Version	XM_015139493.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3729bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-20
Organism	Macaca mulatta(Rhesus monkey)
Product	serine/threonine-protein kinase Nek1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014804907.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Macaca mulatta Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG CAGACAGTAT GTTATCAAGG AAATTAACAT CTCAAGAATG 
TCCAGTAAAG AAAGAGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GTTCTCTCTA CATAGTGATG 
GATTACTGTG AGGGAGGGGA TCTGTTTAAG CGAATAAATG CTCAGAAAGG CGTTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTGAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATTAAA TCTCAGAACA TATTTTTAAC TAAAGATGGA 
ACGGTACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AATAATAAAA GTGACATTTG GGCTCTGGGG TGTGTCCTTT ATGAGCTGTG TACACTTAAA 
CATGCTTTTG AAGCTGGCAG TATGAAAAAC TTGGTACTGA AGATAATATC CGGATCTTTT 
CCACCTGTGT CTTTGCATTA TTCCTATGAT CTCCGCAGTT TGGTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC ACCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACGTTT 
TCAAAGTTTG GATCACAGCC TATACCAGCC AAAAGACCAG CTTCAGGACA AAACTCGATT 
TCTGTTATGC CTGCTCAGAA AATTACAAAG CCTGCCGCTA AATATGGAAT ACCTTTAACA 
TATAAGAAAT ATGGAGAGAA AAAATTATAC GAAAAGAAAC CACTGCAAAA ACATAAACAG 
GCCCATCAAA CTCCAGAGAA GAGAGTGAAT ATTGGAGAAG AAAAGAGGAA AATGTCTGAG 
GAAGCAGCAA GAAAGAGAAG GCTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTATTAGTT TAATGAAGGC TGAACAAATG AAAAGGCAAG AAAAGGAAAG GTTGGAAAGA 
ATAAATAGGG CCAGGGAACA AGGATGGAGA AATGTGCTAA GTGCTGGTGG AGGTGGTGAA 
GTAAAGGCTC CTTTTCTTGG CAGTGGAGGG ACTATAGCTC CATCATCTTT TTCTTCTCGA 
GGACAGTATG AACATTACCA TGCCATTTTT GACCAAATGC AGCAACAGAG AGCAGAAGAT 
AATGAAGCTA AATGGAAAAG AGAAATATAT GGTCGAGGTC TTCCAGAAAG GCAAAAAGGG 
CAGCTAGCTG TAGAAAGAGC TAAACAAGTA GAAGAGTTCC TGCAGCGAAA ACGGGAAGCT 
ATGCAGAATA AAGCTCGAGC CGAAGGACAT ATGGGAATCC TGCAAAACCT GGCAGCTATG 
TATGGAGGCA GGCCCAGCTC TTCAAGAGGA GGGAAGCCAA GGAACAAAGA GGAAGAGGTT 
TATCTGGCAA GACTGAGGCA AATAAGACTA CAGAATTTCA ATGAACGCCA ACAGATTAAA 
GCCAAACTTC GTGGTGAAAA GAAAGAAGCT AATCGTTCTG AAGGACAAGA AGGAAGTGAA 
GAGGCTGACA TGAGGCGCAA AAAAATCGAA TCCCTGAAGG CCCATGCAAA TGCACGTGCT 
GCTGTACTAA AAGAACAACT AGAACGAAAG AGAAAGGAAG CTTATGAGAG AGAAAAAAAA 
GTGTGGGAAG AGCATTTGGT GGCTAAAGGA GTTAAGAGTT CTGATGTTTC TCCACCTTTG 
GGACAGCATG AAACAGGTGG CTCTCCATCA AAGCAACAGA TGAGATCGGT TATTTCTGTG 
ACTTCAGCTT TGAAAGAAGT TGGCGTGGAC AGTAGTTTAA CTGATATCCG GGAAATTTCA 
GAAGAAATGC AAAAGACCAA CAATGCTATT TCAAGTAAGC GAGAAATACT TCGTAGATTA 
AATGAAAATC TTAAAGCTCA AGAAGATGAA AAAGGAAAGC AGCATCTCTC TGATACTTGT 
GAGATAAATG TTCTTGAAGA TGCCAAAGAG CATGAAAAAG AAAAATCAGT TTCATCTGAT 
CGCAAGAAGT GGGAGGCAGG AGGTCAACTT GTGATTCCTC TGGATGAGTT AACACTAGAT 
ACATCCTTCT CTACAACTGA AAGACATACA GTGGGAGAAG TTATTAAATT AGATCCTAAT 
GGATCTCCAA GAAGAGCCTG GGGGAAAAGT CCGACAGATT CTGTTCTGAA GATACTTGGA 
GAAGCTGAAC TACAACTTCA GACAGAACTA TTAGAAAATA CAACTATTAG AAGTGATATT 
TCTCCTGAAG GGGAAAAGTA CAAACCCTTA ATTACTGAAG AAAAAAAAGT GCAATGTATT 
TCACATGAAA TAAACCCATC AGCTATTGTT GATTCTCCTG TTGAGACAGA AAGCCCCGAG 
TGTACTGAGG CATCTCCACA GATGTCATTG AAACTGGAAG GAAATTTAGA AGAACCTGAT 
GATTTGGAAA CAGAAGTTCT ACAAGAGCCA AGTGGAACAA ACAAAGATGA GAGCTTGCCA 
TGCACTGTTA CTGATGTGTG GATTAGTGAG GAAAAAGAAG CAAAGGAAAC TCAGTTGGAA 
GATAGGATCA CCATTCAAGA AAATGAAGTT TCTGAGGATG GAGTCTCGAG TACTGTGGAC 
CAACTTAGTG ACATTCATAT CGAGACGGGA GCCAATGATT CTCAGCACTC TAAATGTGAT 
GTAGATAAGT CTGTGCAACC AGAGCCATTT TTCCATAAAG TGGTTCATTC TGAACACTTG 
AACTTAGTCC CTCAAGTTCA ATCAGTTCAG TGTTCACCAG AAGAATCCTT TGCATTTCGA 
TCTCACTCGC ATTTACCACC AAAAAATAAA AACAAGAATT CCTTGCTGAT TGGACTTTCA 
ACTGGTCTGT TTGATGCAAA CAACCCAAAG ATGTTAAGGA CATGTTCACT TCCAGATCTC 
TCAAAGCTGT TCAGAACCCT TATGGATGTT CCCACCGTAG GAGATGTTCG TCAAGACAAT 
CTTGAAATAG ATGAAATTGA AGATGAAAAC ATTAAAGAAG GACCTTCTGA TTCTGAAGAC 
ATTGTGTTTG AAGAAACTGA CACAGATTTA CAAGAGCTGC AGGCCTCAAT GGAACAGTTA 
CTTAGGGAAC AACCTAGTGA AGAATACAGT GAAGAAGAAG AGTCAGTCTT AAAGAACAGT 
GATGTGGAGC CAACTGCAAA TGGGACAGAT GTGGCGGATG AAGATGACAA TCCCAGCAGT 
GAAAGTGCCC TGAACGAAGA ATGGCACTCA GATAACAGTG ATGGGGAAAA TGCTAGTGAA 
TGTGAATACG ATAGTGTCTT TAACCATTTA GAGGAACTGA GACTTCATCT GGAGCAGGAA 
ATGGGCTTTG AAAAATTCTT TGAGGTTTAC GAGAAAATAA AGGCTATTCA TGAAGATGAA 
GATGAAAATA TTGAAATTTG TTCAAAAATA GTTCAAAATA TTTTGGGAAA TGAACATCAG 
CATCTTTATG CCAAGATTCT TCATTTAGTC ATGGCAGATG GAGCCTACCA AGAAGATAAT 
GATGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014994979.1
CDS	481..4209
Translation	MEKYVRLQKIGEGSFGKAILVKSTEDGRQYVIKEINISRMSSKEREESRREVAVLANMKHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGVLFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYELCTLKHAFEAGSMKNLVLKIISGSFPPVSLHYSYDLRSLVSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTFSKFGSQPIPAKRPASGQNSISVMPAQKITKPAAKYGIPLTYKKYGEKKLYEKKPLQKHKQAHQTPEKRVNIGEEKRKMSEEAARKRRLEFIEKEKKQKDQIISLMKAEQMKRQEKERLERINRAREQGWRNVLSAGGGGEVKAPFLGSGGTIAPSSFSSRGQYEHYHAIFDQMQQQRAEDNEAKWKREIYGRGLPERQKGQLAVERAKQVEEFLQRKREAMQNKARAEGHMGILQNLAAMYGGRPSSSRGGKPRNKEEEVYLARLRQIRLQNFNERQQIKAKLRGEKKEANRSEGQEGSEEADMRRKKIESLKAHANARAAVLKEQLERKRKEAYEREKKVWEEHLVAKGVKSSDVSPPLGQHETGGSPSKQQMRSVISVTSALKEVGVDSSLTDIREISEEMQKTNNAISSKREILRRLNENLKAQEDEKGKQHLSDTCEINVLEDAKEHEKEKSVSSDRKKWEAGGQLVIPLDELTLDTSFSTTERHTVGEVIKLDPNGSPRRAWGKSPTDSVLKILGEAELQLQTELLENTTIRSDISPEGEKYKPLITEEKKVQCISHEINPSAIVDSPVETESPECTEASPQMSLKLEGNLEEPDDLETEVLQEPSGTNKDESLPCTVTDVWISEEKEAKETQLEDRITIQENEVSEDGVSSTVDQLSDIHIETGANDSQHSKCDVDKSVQPEPFFHKVVHSEHLNLVPQVQSVQCSPEESFAFRSHSHLPPKNKNKNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDVRQDNLEIDEIEDENIKEGPSDSEDIVFEETDTDLQELQASMEQLLREQPSEEYSEEEESVLKNSDVEPTANGTDVADEDDNPSSESALNEEWHSDNSDGENASECEYDSVFNHLEELRLHLEQEMGFEKFFEVYEKIKAIHEDEDENIEICSKIVQNILGNEHQHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_015139493.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta NIMA-related kinase 1 (NEK1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015139493.1

ORF Insert Sequence:

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG CAGACAGTAT GTTATCAAGG AAATTAACAT CTCAAGAATG 
TCCAGTAAAG AAAGAGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GTTCTCTCTA CATAGTGATG 
GATTACTGTG AGGGAGGGGA TCTGTTTAAG CGAATAAATG CTCAGAAAGG CGTTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTGAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATTAAA TCTCAGAACA TATTTTTAAC TAAAGATGGA 
ACGGTACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AATAATAAAA GTGACATTTG GGCTCTGGGG TGTGTCCTTT ATGAGCTGTG TACACTTAAA 
CATGCTTTTG AAGCTGGCAG TATGAAAAAC TTGGTACTGA AGATAATATC CGGATCTTTT 
CCACCTGTGT CTTTGCATTA TTCCTATGAT CTCCGCAGTT TGGTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC ACCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACGTTT 
TCAAAGTTTG GATCACAGCC TATACCAGCC AAAAGACCAG CTTCAGGACA AAACTCGATT 
TCTGTTATGC CTGCTCAGAA AATTACAAAG CCTGCCGCTA AATATGGAAT ACCTTTAACA 
TATAAGAAAT ATGGAGAGAA AAAATTATAC GAAAAGAAAC CACTGCAAAA ACATAAACAG 
GCCCATCAAA CTCCAGAGAA GAGAGTGAAT ATTGGAGAAG AAAAGAGGAA AATGTCTGAG 
GAAGCAGCAA GAAAGAGAAG GCTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTATTAGTT TAATGAAGGC TGAACAAATG AAAAGGCAAG AAAAGGAAAG GTTGGAAAGA 
ATAAATAGGG CCAGGGAACA AGGATGGAGA AATGTGCTAA GTGCTGGTGG AGGTGGTGAA 
GTAAAGGCTC CTTTTCTTGG CAGTGGAGGG ACTATAGCTC CATCATCTTT TTCTTCTCGA 
GGACAGTATG AACATTACCA TGCCATTTTT GACCAAATGC AGCAACAGAG AGCAGAAGAT 
AATGAAGCTA AATGGAAAAG AGAAATATAT GGTCGAGGTC TTCCAGAAAG GCAAAAAGGG 
CAGCTAGCTG TAGAAAGAGC TAAACAAGTA GAAGAGTTCC TGCAGCGAAA ACGGGAAGCT 
ATGCAGAATA AAGCTCGAGC CGAAGGACAT ATGGGAATCC TGCAAAACCT GGCAGCTATG 
TATGGAGGCA GGCCCAGCTC TTCAAGAGGA GGGAAGCCAA GGAACAAAGA GGAAGAGGTT 
TATCTGGCAA GACTGAGGCA AATAAGACTA CAGAATTTCA ATGAACGCCA ACAGATTAAA 
GCCAAACTTC GTGGTGAAAA GAAAGAAGCT AATCGTTCTG AAGGACAAGA AGGAAGTGAA 
GAGGCTGACA TGAGGCGCAA AAAAATCGAA TCCCTGAAGG CCCATGCAAA TGCACGTGCT 
GCTGTACTAA AAGAACAACT AGAACGAAAG AGAAAGGAAG CTTATGAGAG AGAAAAAAAA 
GTGTGGGAAG AGCATTTGGT GGCTAAAGGA GTTAAGAGTT CTGATGTTTC TCCACCTTTG 
GGACAGCATG AAACAGGTGG CTCTCCATCA AAGCAACAGA TGAGATCGGT TATTTCTGTG 
ACTTCAGCTT TGAAAGAAGT TGGCGTGGAC AGTAGTTTAA CTGATATCCG GGAAATTTCA 
GAAGAAATGC AAAAGACCAA CAATGCTATT TCAAGTAAGC GAGAAATACT TCGTAGATTA 
AATGAAAATC TTAAAGCTCA AGAAGATGAA AAAGGAAAGC AGCATCTCTC TGATACTTGT 
GAGATAAATG TTCTTGAAGA TGCCAAAGAG CATGAAAAAG AAAAATCAGT TTCATCTGAT 
CGCAAGAAGT GGGAGGCAGG AGGTCAACTT GTGATTCCTC TGGATGAGTT AACACTAGAT 
ACATCCTTCT CTACAACTGA AAGACATACA GTGGGAGAAG TTATTAAATT AGATCCTAAT 
GGATCTCCAA GAAGAGCCTG GGGGAAAAGT CCGACAGATT CTGTTCTGAA GATACTTGGA 
GAAGCTGAAC TACAACTTCA GACAGAACTA TTAGAAAATA CAACTATTAG AAGTGATATT 
TCTCCTGAAG GGGAAAAGTA CAAACCCTTA ATTACTGAAG AAAAAAAAGT GCAATGTATT 
TCACATGAAA TAAACCCATC AGCTATTGTT GATTCTCCTG TTGAGACAGA AAGCCCCGAG 
TGTACTGAGG CATCTCCACA GATGTCATTG AAACTGGAAG GAAATTTAGA AGAACCTGAT 
GATTTGGAAA CAGAAGTTCT ACAAGAGCCA AGTGGAACAA ACAAAGATGA GAGCTTGCCA 
TGCACTGTTA CTGATGTGTG GATTAGTGAG GAAAAAGAAG CAAAGGAAAC TCAGTTGGAA 
GATAGGATCA CCATTCAAGA AAATGAAGTT TCTGAGGATG GAGTCTCGAG TACTGTGGAC 
CAACTTAGTG ACATTCATAT CGAGACGGGA GCCAATGATT CTCAGCACTC TAAATGTGAT 
GTAGATAAGT CTGTGCAACC AGAGCCATTT TTCCATAAAG TGGTTCATTC TGAACACTTG 
AACTTAGTCC CTCAAGTTCA ATCAGTTCAG TGTTCACCAG AAGAATCCTT TGCATTTCGA 
TCTCACTCGC ATTTACCACC AAAAAATAAA AACAAGAATT CCTTGCTGAT TGGACTTTCA 
ACTGGTCTGT TTGATGCAAA CAACCCAAAG ATGTTAAGGA CATGTTCACT TCCAGATCTC 
TCAAAGCTGT TCAGAACCCT TATGGATGTT CCCACCGTAG GAGATGTTCG TCAAGACAAT 
CTTGAAATAG ATGAAATTGA AGATGAAAAC ATTAAAGAAG GACCTTCTGA TTCTGAAGAC 
ATTGTGTTTG AAGAAACTGA CACAGATTTA CAAGAGCTGC AGGCCTCAAT GGAACAGTTA 
CTTAGGGAAC AACCTAGTGA AGAATACAGT GAAGAAGAAG AGTCAGTCTT AAAGAACAGT 
GATGTGGAGC CAACTGCAAA TGGGACAGAT GTGGCGGATG AAGATGACAA TCCCAGCAGT 
GAAAGTGCCC TGAACGAAGA ATGGCACTCA GATAACAGTG ATGGGGAAAA TGCTAGTGAA 
TGTGAATACG ATAGTGTCTT TAACCATTTA GAGGAACTGA GACTTCATCT GGAGCAGGAA 
ATGGGCTTTG AAAAATTCTT TGAGGTTTAC GAGAAAATAA AGGCTATTCA TGAAGATGAA 
GATGAAAATA TTGAAATTTG TTCAAAAATA GTTCAAAATA TTTTGGGAAA TGAACATCAG 
CATCTTTATG CCAAGATTCT TCATTTAGTC ATGGCAGATG GAGCCTACCA AGAAGATAAT 
GATGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_001083043.3 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_001083043.2 Macaca mulatta NIMA-related kinase 1 (NEK1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OMb34269
Clone ID Related Accession (Same CDS sequence)	XM_015139493.1 , XM_001083043.3
Accession Version	XM_001083043.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3729bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-20
Organism	Macaca mulatta(Rhesus monkey)
Product	serine/threonine-protein kinase Nek1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014804907.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 21, 2015 this sequence version replaced XM_001083043.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Macaca mulatta Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG CAGACAGTAT GTTATCAAGG AAATTAACAT CTCAAGAATG 
TCCAGTAAAG AAAGAGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GTTCTCTCTA CATAGTGATG 
GATTACTGTG AGGGAGGGGA TCTGTTTAAG CGAATAAATG CTCAGAAAGG CGTTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTGAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATTAAA TCTCAGAACA TATTTTTAAC TAAAGATGGA 
ACGGTACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AATAATAAAA GTGACATTTG GGCTCTGGGG TGTGTCCTTT ATGAGCTGTG TACACTTAAA 
CATGCTTTTG AAGCTGGCAG TATGAAAAAC TTGGTACTGA AGATAATATC CGGATCTTTT 
CCACCTGTGT CTTTGCATTA TTCCTATGAT CTCCGCAGTT TGGTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC ACCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACGTTT 
TCAAAGTTTG GATCACAGCC TATACCAGCC AAAAGACCAG CTTCAGGACA AAACTCGATT 
TCTGTTATGC CTGCTCAGAA AATTACAAAG CCTGCCGCTA AATATGGAAT ACCTTTAACA 
TATAAGAAAT ATGGAGAGAA AAAATTATAC GAAAAGAAAC CACTGCAAAA ACATAAACAG 
GCCCATCAAA CTCCAGAGAA GAGAGTGAAT ATTGGAGAAG AAAAGAGGAA AATGTCTGAG 
GAAGCAGCAA GAAAGAGAAG GCTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTATTAGTT TAATGAAGGC TGAACAAATG AAAAGGCAAG AAAAGGAAAG GTTGGAAAGA 
ATAAATAGGG CCAGGGAACA AGGATGGAGA AATGTGCTAA GTGCTGGTGG AGGTGGTGAA 
GTAAAGGCTC CTTTTCTTGG CAGTGGAGGG ACTATAGCTC CATCATCTTT TTCTTCTCGA 
GGACAGTATG AACATTACCA TGCCATTTTT GACCAAATGC AGCAACAGAG AGCAGAAGAT 
AATGAAGCTA AATGGAAAAG AGAAATATAT GGTCGAGGTC TTCCAGAAAG GCAAAAAGGG 
CAGCTAGCTG TAGAAAGAGC TAAACAAGTA GAAGAGTTCC TGCAGCGAAA ACGGGAAGCT 
ATGCAGAATA AAGCTCGAGC CGAAGGACAT ATGGGAATCC TGCAAAACCT GGCAGCTATG 
TATGGAGGCA GGCCCAGCTC TTCAAGAGGA GGGAAGCCAA GGAACAAAGA GGAAGAGGTT 
TATCTGGCAA GACTGAGGCA AATAAGACTA CAGAATTTCA ATGAACGCCA ACAGATTAAA 
GCCAAACTTC GTGGTGAAAA GAAAGAAGCT AATCGTTCTG AAGGACAAGA AGGAAGTGAA 
GAGGCTGACA TGAGGCGCAA AAAAATCGAA TCCCTGAAGG CCCATGCAAA TGCACGTGCT 
GCTGTACTAA AAGAACAACT AGAACGAAAG AGAAAGGAAG CTTATGAGAG AGAAAAAAAA 
GTGTGGGAAG AGCATTTGGT GGCTAAAGGA GTTAAGAGTT CTGATGTTTC TCCACCTTTG 
GGACAGCATG AAACAGGTGG CTCTCCATCA AAGCAACAGA TGAGATCGGT TATTTCTGTG 
ACTTCAGCTT TGAAAGAAGT TGGCGTGGAC AGTAGTTTAA CTGATATCCG GGAAATTTCA 
GAAGAAATGC AAAAGACCAA CAATGCTATT TCAAGTAAGC GAGAAATACT TCGTAGATTA 
AATGAAAATC TTAAAGCTCA AGAAGATGAA AAAGGAAAGC AGCATCTCTC TGATACTTGT 
GAGATAAATG TTCTTGAAGA TGCCAAAGAG CATGAAAAAG AAAAATCAGT TTCATCTGAT 
CGCAAGAAGT GGGAGGCAGG AGGTCAACTT GTGATTCCTC TGGATGAGTT AACACTAGAT 
ACATCCTTCT CTACAACTGA AAGACATACA GTGGGAGAAG TTATTAAATT AGATCCTAAT 
GGATCTCCAA GAAGAGCCTG GGGGAAAAGT CCGACAGATT CTGTTCTGAA GATACTTGGA 
GAAGCTGAAC TACAACTTCA GACAGAACTA TTAGAAAATA CAACTATTAG AAGTGATATT 
TCTCCTGAAG GGGAAAAGTA CAAACCCTTA ATTACTGAAG AAAAAAAAGT GCAATGTATT 
TCACATGAAA TAAACCCATC AGCTATTGTT GATTCTCCTG TTGAGACAGA AAGCCCCGAG 
TGTACTGAGG CATCTCCACA GATGTCATTG AAACTGGAAG GAAATTTAGA AGAACCTGAT 
GATTTGGAAA CAGAAGTTCT ACAAGAGCCA AGTGGAACAA ACAAAGATGA GAGCTTGCCA 
TGCACTGTTA CTGATGTGTG GATTAGTGAG GAAAAAGAAG CAAAGGAAAC TCAGTTGGAA 
GATAGGATCA CCATTCAAGA AAATGAAGTT TCTGAGGATG GAGTCTCGAG TACTGTGGAC 
CAACTTAGTG ACATTCATAT CGAGACGGGA GCCAATGATT CTCAGCACTC TAAATGTGAT 
GTAGATAAGT CTGTGCAACC AGAGCCATTT TTCCATAAAG TGGTTCATTC TGAACACTTG 
AACTTAGTCC CTCAAGTTCA ATCAGTTCAG TGTTCACCAG AAGAATCCTT TGCATTTCGA 
TCTCACTCGC ATTTACCACC AAAAAATAAA AACAAGAATT CCTTGCTGAT TGGACTTTCA 
ACTGGTCTGT TTGATGCAAA CAACCCAAAG ATGTTAAGGA CATGTTCACT TCCAGATCTC 
TCAAAGCTGT TCAGAACCCT TATGGATGTT CCCACCGTAG GAGATGTTCG TCAAGACAAT 
CTTGAAATAG ATGAAATTGA AGATGAAAAC ATTAAAGAAG GACCTTCTGA TTCTGAAGAC 
ATTGTGTTTG AAGAAACTGA CACAGATTTA CAAGAGCTGC AGGCCTCAAT GGAACAGTTA 
CTTAGGGAAC AACCTAGTGA AGAATACAGT GAAGAAGAAG AGTCAGTCTT AAAGAACAGT 
GATGTGGAGC CAACTGCAAA TGGGACAGAT GTGGCGGATG AAGATGACAA TCCCAGCAGT 
GAAAGTGCCC TGAACGAAGA ATGGCACTCA GATAACAGTG ATGGGGAAAA TGCTAGTGAA 
TGTGAATACG ATAGTGTCTT TAACCATTTA GAGGAACTGA GACTTCATCT GGAGCAGGAA 
ATGGGCTTTG AAAAATTCTT TGAGGTTTAC GAGAAAATAA AGGCTATTCA TGAAGATGAA 
GATGAAAATA TTGAAATTTG TTCAAAAATA GTTCAAAATA TTTTGGGAAA TGAACATCAG 
CATCTTTATG CCAAGATTCT TCATTTAGTC ATGGCAGATG GAGCCTACCA AGAAGATAAT 
GATGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001083043.2
CDS	570..4298
Translation	MEKYVRLQKIGEGSFGKAILVKSTEDGRQYVIKEINISRMSSKEREESRREVAVLANMKHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGVLFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYELCTLKHAFEAGSMKNLVLKIISGSFPPVSLHYSYDLRSLVSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTFSKFGSQPIPAKRPASGQNSISVMPAQKITKPAAKYGIPLTYKKYGEKKLYEKKPLQKHKQAHQTPEKRVNIGEEKRKMSEEAARKRRLEFIEKEKKQKDQIISLMKAEQMKRQEKERLERINRAREQGWRNVLSAGGGGEVKAPFLGSGGTIAPSSFSSRGQYEHYHAIFDQMQQQRAEDNEAKWKREIYGRGLPERQKGQLAVERAKQVEEFLQRKREAMQNKARAEGHMGILQNLAAMYGGRPSSSRGGKPRNKEEEVYLARLRQIRLQNFNERQQIKAKLRGEKKEANRSEGQEGSEEADMRRKKIESLKAHANARAAVLKEQLERKRKEAYEREKKVWEEHLVAKGVKSSDVSPPLGQHETGGSPSKQQMRSVISVTSALKEVGVDSSLTDIREISEEMQKTNNAISSKREILRRLNENLKAQEDEKGKQHLSDTCEINVLEDAKEHEKEKSVSSDRKKWEAGGQLVIPLDELTLDTSFSTTERHTVGEVIKLDPNGSPRRAWGKSPTDSVLKILGEAELQLQTELLENTTIRSDISPEGEKYKPLITEEKKVQCISHEINPSAIVDSPVETESPECTEASPQMSLKLEGNLEEPDDLETEVLQEPSGTNKDESLPCTVTDVWISEEKEAKETQLEDRITIQENEVSEDGVSSTVDQLSDIHIETGANDSQHSKCDVDKSVQPEPFFHKVVHSEHLNLVPQVQSVQCSPEESFAFRSHSHLPPKNKNKNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDVRQDNLEIDEIEDENIKEGPSDSEDIVFEETDTDLQELQASMEQLLREQPSEEYSEEEESVLKNSDVEPTANGTDVADEDDNPSSESALNEEWHSDNSDGENASECEYDSVFNHLEELRLHLEQEMGFEKFFEVYEKIKAIHEDEDENIEICSKIVQNILGNEHQHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_001083043.3
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta NIMA-related kinase 1 (NEK1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001083043.3

ORF Insert Sequence:

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG CAGACAGTAT GTTATCAAGG AAATTAACAT CTCAAGAATG 
TCCAGTAAAG AAAGAGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GTTCTCTCTA CATAGTGATG 
GATTACTGTG AGGGAGGGGA TCTGTTTAAG CGAATAAATG CTCAGAAAGG CGTTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTGAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATTAAA TCTCAGAACA TATTTTTAAC TAAAGATGGA 
ACGGTACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AATAATAAAA GTGACATTTG GGCTCTGGGG TGTGTCCTTT ATGAGCTGTG TACACTTAAA 
CATGCTTTTG AAGCTGGCAG TATGAAAAAC TTGGTACTGA AGATAATATC CGGATCTTTT 
CCACCTGTGT CTTTGCATTA TTCCTATGAT CTCCGCAGTT TGGTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC ACCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACGTTT 
TCAAAGTTTG GATCACAGCC TATACCAGCC AAAAGACCAG CTTCAGGACA AAACTCGATT 
TCTGTTATGC CTGCTCAGAA AATTACAAAG CCTGCCGCTA AATATGGAAT ACCTTTAACA 
TATAAGAAAT ATGGAGAGAA AAAATTATAC GAAAAGAAAC CACTGCAAAA ACATAAACAG 
GCCCATCAAA CTCCAGAGAA GAGAGTGAAT ATTGGAGAAG AAAAGAGGAA AATGTCTGAG 
GAAGCAGCAA GAAAGAGAAG GCTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTATTAGTT TAATGAAGGC TGAACAAATG AAAAGGCAAG AAAAGGAAAG GTTGGAAAGA 
ATAAATAGGG CCAGGGAACA AGGATGGAGA AATGTGCTAA GTGCTGGTGG AGGTGGTGAA 
GTAAAGGCTC CTTTTCTTGG CAGTGGAGGG ACTATAGCTC CATCATCTTT TTCTTCTCGA 
GGACAGTATG AACATTACCA TGCCATTTTT GACCAAATGC AGCAACAGAG AGCAGAAGAT 
AATGAAGCTA AATGGAAAAG AGAAATATAT GGTCGAGGTC TTCCAGAAAG GCAAAAAGGG 
CAGCTAGCTG TAGAAAGAGC TAAACAAGTA GAAGAGTTCC TGCAGCGAAA ACGGGAAGCT 
ATGCAGAATA AAGCTCGAGC CGAAGGACAT ATGGGAATCC TGCAAAACCT GGCAGCTATG 
TATGGAGGCA GGCCCAGCTC TTCAAGAGGA GGGAAGCCAA GGAACAAAGA GGAAGAGGTT 
TATCTGGCAA GACTGAGGCA AATAAGACTA CAGAATTTCA ATGAACGCCA ACAGATTAAA 
GCCAAACTTC GTGGTGAAAA GAAAGAAGCT AATCGTTCTG AAGGACAAGA AGGAAGTGAA 
GAGGCTGACA TGAGGCGCAA AAAAATCGAA TCCCTGAAGG CCCATGCAAA TGCACGTGCT 
GCTGTACTAA AAGAACAACT AGAACGAAAG AGAAAGGAAG CTTATGAGAG AGAAAAAAAA 
GTGTGGGAAG AGCATTTGGT GGCTAAAGGA GTTAAGAGTT CTGATGTTTC TCCACCTTTG 
GGACAGCATG AAACAGGTGG CTCTCCATCA AAGCAACAGA TGAGATCGGT TATTTCTGTG 
ACTTCAGCTT TGAAAGAAGT TGGCGTGGAC AGTAGTTTAA CTGATATCCG GGAAATTTCA 
GAAGAAATGC AAAAGACCAA CAATGCTATT TCAAGTAAGC GAGAAATACT TCGTAGATTA 
AATGAAAATC TTAAAGCTCA AGAAGATGAA AAAGGAAAGC AGCATCTCTC TGATACTTGT 
GAGATAAATG TTCTTGAAGA TGCCAAAGAG CATGAAAAAG AAAAATCAGT TTCATCTGAT 
CGCAAGAAGT GGGAGGCAGG AGGTCAACTT GTGATTCCTC TGGATGAGTT AACACTAGAT 
ACATCCTTCT CTACAACTGA AAGACATACA GTGGGAGAAG TTATTAAATT AGATCCTAAT 
GGATCTCCAA GAAGAGCCTG GGGGAAAAGT CCGACAGATT CTGTTCTGAA GATACTTGGA 
GAAGCTGAAC TACAACTTCA GACAGAACTA TTAGAAAATA CAACTATTAG AAGTGATATT 
TCTCCTGAAG GGGAAAAGTA CAAACCCTTA ATTACTGAAG AAAAAAAAGT GCAATGTATT 
TCACATGAAA TAAACCCATC AGCTATTGTT GATTCTCCTG TTGAGACAGA AAGCCCCGAG 
TGTACTGAGG CATCTCCACA GATGTCATTG AAACTGGAAG GAAATTTAGA AGAACCTGAT 
GATTTGGAAA CAGAAGTTCT ACAAGAGCCA AGTGGAACAA ACAAAGATGA GAGCTTGCCA 
TGCACTGTTA CTGATGTGTG GATTAGTGAG GAAAAAGAAG CAAAGGAAAC TCAGTTGGAA 
GATAGGATCA CCATTCAAGA AAATGAAGTT TCTGAGGATG GAGTCTCGAG TACTGTGGAC 
CAACTTAGTG ACATTCATAT CGAGACGGGA GCCAATGATT CTCAGCACTC TAAATGTGAT 
GTAGATAAGT CTGTGCAACC AGAGCCATTT TTCCATAAAG TGGTTCATTC TGAACACTTG 
AACTTAGTCC CTCAAGTTCA ATCAGTTCAG TGTTCACCAG AAGAATCCTT TGCATTTCGA 
TCTCACTCGC ATTTACCACC AAAAAATAAA AACAAGAATT CCTTGCTGAT TGGACTTTCA 
ACTGGTCTGT TTGATGCAAA CAACCCAAAG ATGTTAAGGA CATGTTCACT TCCAGATCTC 
TCAAAGCTGT TCAGAACCCT TATGGATGTT CCCACCGTAG GAGATGTTCG TCAAGACAAT 
CTTGAAATAG ATGAAATTGA AGATGAAAAC ATTAAAGAAG GACCTTCTGA TTCTGAAGAC 
ATTGTGTTTG AAGAAACTGA CACAGATTTA CAAGAGCTGC AGGCCTCAAT GGAACAGTTA 
CTTAGGGAAC AACCTAGTGA AGAATACAGT GAAGAAGAAG AGTCAGTCTT AAAGAACAGT 
GATGTGGAGC CAACTGCAAA TGGGACAGAT GTGGCGGATG AAGATGACAA TCCCAGCAGT 
GAAAGTGCCC TGAACGAAGA ATGGCACTCA GATAACAGTG ATGGGGAAAA TGCTAGTGAA 
TGTGAATACG ATAGTGTCTT TAACCATTTA GAGGAACTGA GACTTCATCT GGAGCAGGAA 
ATGGGCTTTG AAAAATTCTT TGAGGTTTAC GAGAAAATAA AGGCTATTCA TGAAGATGAA 
GATGAAAATA TTGAAATTTG TTCAAAAATA GTTCAAAATA TTTTGGGAAA TGAACATCAG 
CATCTTTATG CCAAGATTCT TCATTTAGTC ATGGCAGATG GAGCCTACCA AGAAGATAAT 
GATGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_015139494.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_014994980.1 Macaca mulatta NIMA-related kinase 1 (NEK1), transcript variant X6, mRNA.

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CloneID	OMb34270
Clone ID Related Accession (Same CDS sequence)	XM_015139494.1
Accession Version	XM_015139494.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3645bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-20
Organism	Macaca mulatta(Rhesus monkey)
Product	serine/threonine-protein kinase Nek1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014804907.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Macaca mulatta Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG CAGACAGTAT GTTATCAAGG AAATTAACAT CTCAAGAATG 
TCCAGTAAAG AAAGAGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GTTCTCTCTA CATAGTGATG 
GATTACTGTG AGGGAGGGGA TCTGTTTAAG CGAATAAATG CTCAGAAAGG CGTTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTGAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATTAAA TCTCAGAACA TATTTTTAAC TAAAGATGGA 
ACGGTACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AATAATAAAA GTGACATTTG GGCTCTGGGG TGTGTCCTTT ATGAGCTGTG TACACTTAAA 
CATGCTTTTG AAGCTGGCAG TATGAAAAAC TTGGTACTGA AGATAATATC CGGATCTTTT 
CCACCTGTGT CTTTGCATTA TTCCTATGAT CTCCGCAGTT TGGTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC ACCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACGTTT 
TCAAAGTTTG GATCACAGCC TATACCAGCC AAAAGACCAG CTTCAGGACA AAACTCGATT 
TCTGTTATGC CTGCTCAGAA AATTACAAAG CCTGCCGCTA AATATGGAAT ACCTTTAACA 
TATAAGAAAT ATGGAGAGAA AAAATTATAC GAAAAGAAAC CACTGCAAAA ACATAAACAG 
GCCCATCAAA CTCCAGAGAA GAGAGTGAAT ATTGGAGAAG AAAAGAGGAA AATGTCTGAG 
GAAGCAGCAA GAAAGAGAAG GCTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTATTAGTT TAATGAAGGC TGAACAAATG AAAAGGCAAG AAAAGGAAAG GTTGGAAAGA 
ATAAATAGGG CCAGGGAACA AGGATGGAGA AATGTGCTAA GTGCTGGTGG AGGTGGTGAA 
GTAAAGGCTC CTTTTCTTGG CAGTGGAGGG ACTATAGCTC CATCATCTTT TTCTTCTCGA 
GGACAGTATG AACATTACCA TGCCATTTTT GACCAAATGC AGCAACAGAG AGCAGAAGAT 
AATGAAGCTA AATGGAAAAG AGAAATATAT GGTCGAGGTC TTCCAGAAAG GCAAAAAGGG 
CAGCTAGCTG TAGAAAGAGC TAAACAAGTA GAAGAGTTCC TGCAGCGAAA ACGGGAAGCT 
ATGCAGAATA AAGCTCGAGC CGAAGGACAT ATGGTTTATC TGGCAAGACT GAGGCAAATA 
AGACTACAGA ATTTCAATGA ACGCCAACAG ATTAAAGCCA AACTTCGTGG TGAAAAGAAA 
GAAGCTAATC GTTCTGAAGG ACAAGAAGGA AGTGAAGAGG CTGACATGAG GCGCAAAAAA 
ATCGAATCCC TGAAGGCCCA TGCAAATGCA CGTGCTGCTG TACTAAAAGA ACAACTAGAA 
CGAAAGAGAA AGGAAGCTTA TGAGAGAGAA AAAAAAGTGT GGGAAGAGCA TTTGGTGGCT 
AAAGGAGTTA AGAGTTCTGA TGTTTCTCCA CCTTTGGGAC AGCATGAAAC AGGTGGCTCT 
CCATCAAAGC AACAGATGAG ATCGGTTATT TCTGTGACTT CAGCTTTGAA AGAAGTTGGC 
GTGGACAGTA GTTTAACTGA TATCCGGGAA ATTTCAGAAG AAATGCAAAA GACCAACAAT 
GCTATTTCAA GTAAGCGAGA AATACTTCGT AGATTAAATG AAAATCTTAA AGCTCAAGAA 
GATGAAAAAG GAAAGCAGCA TCTCTCTGAT ACTTGTGAGA TAAATGTTCT TGAAGATGCC 
AAAGAGCATG AAAAAGAAAA ATCAGTTTCA TCTGATCGCA AGAAGTGGGA GGCAGGAGGT 
CAACTTGTGA TTCCTCTGGA TGAGTTAACA CTAGATACAT CCTTCTCTAC AACTGAAAGA 
CATACAGTGG GAGAAGTTAT TAAATTAGAT CCTAATGGAT CTCCAAGAAG AGCCTGGGGG 
AAAAGTCCGA CAGATTCTGT TCTGAAGATA CTTGGAGAAG CTGAACTACA ACTTCAGACA 
GAACTATTAG AAAATACAAC TATTAGAAGT GATATTTCTC CTGAAGGGGA AAAGTACAAA 
CCCTTAATTA CTGAAGAAAA AAAAGTGCAA TGTATTTCAC ATGAAATAAA CCCATCAGCT 
ATTGTTGATT CTCCTGTTGA GACAGAAAGC CCCGAGTGTA CTGAGGCATC TCCACAGATG 
TCATTGAAAC TGGAAGGAAA TTTAGAAGAA CCTGATGATT TGGAAACAGA AGTTCTACAA 
GAGCCAAGTG GAACAAACAA AGATGAGAGC TTGCCATGCA CTGTTACTGA TGTGTGGATT 
AGTGAGGAAA AAGAAGCAAA GGAAACTCAG TTGGAAGATA GGATCACCAT TCAAGAAAAT 
GAAGTTTCTG AGGATGGAGT CTCGAGTACT GTGGACCAAC TTAGTGACAT TCATATCGAG 
ACGGGAGCCA ATGATTCTCA GCACTCTAAA TGTGATGTAG ATAAGTCTGT GCAACCAGAG 
CCATTTTTCC ATAAAGTGGT TCATTCTGAA CACTTGAACT TAGTCCCTCA AGTTCAATCA 
GTTCAGTGTT CACCAGAAGA ATCCTTTGCA TTTCGATCTC ACTCGCATTT ACCACCAAAA 
AATAAAAACA AGAATTCCTT GCTGATTGGA CTTTCAACTG GTCTGTTTGA TGCAAACAAC 
CCAAAGATGT TAAGGACATG TTCACTTCCA GATCTCTCAA AGCTGTTCAG AACCCTTATG 
GATGTTCCCA CCGTAGGAGA TGTTCGTCAA GACAATCTTG AAATAGATGA AATTGAAGAT 
GAAAACATTA AAGAAGGACC TTCTGATTCT GAAGACATTG TGTTTGAAGA AACTGACACA 
GATTTACAAG AGCTGCAGGC CTCAATGGAA CAGTTACTTA GGGAACAACC TAGTGAAGAA 
TACAGTGAAG AAGAAGAGTC AGTCTTAAAG AACAGTGATG TGGAGCCAAC TGCAAATGGG 
ACAGATGTGG CGGATGAAGA TGACAATCCC AGCAGTGAAA GTGCCCTGAA CGAAGAATGG 
CACTCAGATA ACAGTGATGG GGAAAATGCT AGTGAATGTG AATACGATAG TGTCTTTAAC 
CATTTAGAGG AACTGAGACT TCATCTGGAG CAGGAAATGG GCTTTGAAAA ATTCTTTGAG 
GTTTACGAGA AAATAAAGGC TATTCATGAA GATGAAGATG AAAATATTGA AATTTGTTCA 
AAAATAGTTC AAAATATTTT GGGAAATGAA CATCAGCATC TTTATGCCAA GATTCTTCAT 
TTAGTCATGG CAGATGGAGC CTACCAAGAA GATAATGATG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014994980.1
CDS	645..4289
Translation	MEKYVRLQKIGEGSFGKAILVKSTEDGRQYVIKEINISRMSSKEREESRREVAVLANMKHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGVLFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYELCTLKHAFEAGSMKNLVLKIISGSFPPVSLHYSYDLRSLVSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTFSKFGSQPIPAKRPASGQNSISVMPAQKITKPAAKYGIPLTYKKYGEKKLYEKKPLQKHKQAHQTPEKRVNIGEEKRKMSEEAARKRRLEFIEKEKKQKDQIISLMKAEQMKRQEKERLERINRAREQGWRNVLSAGGGGEVKAPFLGSGGTIAPSSFSSRGQYEHYHAIFDQMQQQRAEDNEAKWKREIYGRGLPERQKGQLAVERAKQVEEFLQRKREAMQNKARAEGHMVYLARLRQIRLQNFNERQQIKAKLRGEKKEANRSEGQEGSEEADMRRKKIESLKAHANARAAVLKEQLERKRKEAYEREKKVWEEHLVAKGVKSSDVSPPLGQHETGGSPSKQQMRSVISVTSALKEVGVDSSLTDIREISEEMQKTNNAISSKREILRRLNENLKAQEDEKGKQHLSDTCEINVLEDAKEHEKEKSVSSDRKKWEAGGQLVIPLDELTLDTSFSTTERHTVGEVIKLDPNGSPRRAWGKSPTDSVLKILGEAELQLQTELLENTTIRSDISPEGEKYKPLITEEKKVQCISHEINPSAIVDSPVETESPECTEASPQMSLKLEGNLEEPDDLETEVLQEPSGTNKDESLPCTVTDVWISEEKEAKETQLEDRITIQENEVSEDGVSSTVDQLSDIHIETGANDSQHSKCDVDKSVQPEPFFHKVVHSEHLNLVPQVQSVQCSPEESFAFRSHSHLPPKNKNKNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDVRQDNLEIDEIEDENIKEGPSDSEDIVFEETDTDLQELQASMEQLLREQPSEEYSEEEESVLKNSDVEPTANGTDVADEDDNPSSESALNEEWHSDNSDGENASECEYDSVFNHLEELRLHLEQEMGFEKFFEVYEKIKAIHEDEDENIEICSKIVQNILGNEHQHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_015139494.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta NIMA-related kinase 1 (NEK1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015139494.1

ORF Insert Sequence:

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG CAGACAGTAT GTTATCAAGG AAATTAACAT CTCAAGAATG 
TCCAGTAAAG AAAGAGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GTTCTCTCTA CATAGTGATG 
GATTACTGTG AGGGAGGGGA TCTGTTTAAG CGAATAAATG CTCAGAAAGG CGTTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTGAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATTAAA TCTCAGAACA TATTTTTAAC TAAAGATGGA 
ACGGTACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AATAATAAAA GTGACATTTG GGCTCTGGGG TGTGTCCTTT ATGAGCTGTG TACACTTAAA 
CATGCTTTTG AAGCTGGCAG TATGAAAAAC TTGGTACTGA AGATAATATC CGGATCTTTT 
CCACCTGTGT CTTTGCATTA TTCCTATGAT CTCCGCAGTT TGGTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC ACCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACGTTT 
TCAAAGTTTG GATCACAGCC TATACCAGCC AAAAGACCAG CTTCAGGACA AAACTCGATT 
TCTGTTATGC CTGCTCAGAA AATTACAAAG CCTGCCGCTA AATATGGAAT ACCTTTAACA 
TATAAGAAAT ATGGAGAGAA AAAATTATAC GAAAAGAAAC CACTGCAAAA ACATAAACAG 
GCCCATCAAA CTCCAGAGAA GAGAGTGAAT ATTGGAGAAG AAAAGAGGAA AATGTCTGAG 
GAAGCAGCAA GAAAGAGAAG GCTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTATTAGTT TAATGAAGGC TGAACAAATG AAAAGGCAAG AAAAGGAAAG GTTGGAAAGA 
ATAAATAGGG CCAGGGAACA AGGATGGAGA AATGTGCTAA GTGCTGGTGG AGGTGGTGAA 
GTAAAGGCTC CTTTTCTTGG CAGTGGAGGG ACTATAGCTC CATCATCTTT TTCTTCTCGA 
GGACAGTATG AACATTACCA TGCCATTTTT GACCAAATGC AGCAACAGAG AGCAGAAGAT 
AATGAAGCTA AATGGAAAAG AGAAATATAT GGTCGAGGTC TTCCAGAAAG GCAAAAAGGG 
CAGCTAGCTG TAGAAAGAGC TAAACAAGTA GAAGAGTTCC TGCAGCGAAA ACGGGAAGCT 
ATGCAGAATA AAGCTCGAGC CGAAGGACAT ATGGTTTATC TGGCAAGACT GAGGCAAATA 
AGACTACAGA ATTTCAATGA ACGCCAACAG ATTAAAGCCA AACTTCGTGG TGAAAAGAAA 
GAAGCTAATC GTTCTGAAGG ACAAGAAGGA AGTGAAGAGG CTGACATGAG GCGCAAAAAA 
ATCGAATCCC TGAAGGCCCA TGCAAATGCA CGTGCTGCTG TACTAAAAGA ACAACTAGAA 
CGAAAGAGAA AGGAAGCTTA TGAGAGAGAA AAAAAAGTGT GGGAAGAGCA TTTGGTGGCT 
AAAGGAGTTA AGAGTTCTGA TGTTTCTCCA CCTTTGGGAC AGCATGAAAC AGGTGGCTCT 
CCATCAAAGC AACAGATGAG ATCGGTTATT TCTGTGACTT CAGCTTTGAA AGAAGTTGGC 
GTGGACAGTA GTTTAACTGA TATCCGGGAA ATTTCAGAAG AAATGCAAAA GACCAACAAT 
GCTATTTCAA GTAAGCGAGA AATACTTCGT AGATTAAATG AAAATCTTAA AGCTCAAGAA 
GATGAAAAAG GAAAGCAGCA TCTCTCTGAT ACTTGTGAGA TAAATGTTCT TGAAGATGCC 
AAAGAGCATG AAAAAGAAAA ATCAGTTTCA TCTGATCGCA AGAAGTGGGA GGCAGGAGGT 
CAACTTGTGA TTCCTCTGGA TGAGTTAACA CTAGATACAT CCTTCTCTAC AACTGAAAGA 
CATACAGTGG GAGAAGTTAT TAAATTAGAT CCTAATGGAT CTCCAAGAAG AGCCTGGGGG 
AAAAGTCCGA CAGATTCTGT TCTGAAGATA CTTGGAGAAG CTGAACTACA ACTTCAGACA 
GAACTATTAG AAAATACAAC TATTAGAAGT GATATTTCTC CTGAAGGGGA AAAGTACAAA 
CCCTTAATTA CTGAAGAAAA AAAAGTGCAA TGTATTTCAC ATGAAATAAA CCCATCAGCT 
ATTGTTGATT CTCCTGTTGA GACAGAAAGC CCCGAGTGTA CTGAGGCATC TCCACAGATG 
TCATTGAAAC TGGAAGGAAA TTTAGAAGAA CCTGATGATT TGGAAACAGA AGTTCTACAA 
GAGCCAAGTG GAACAAACAA AGATGAGAGC TTGCCATGCA CTGTTACTGA TGTGTGGATT 
AGTGAGGAAA AAGAAGCAAA GGAAACTCAG TTGGAAGATA GGATCACCAT TCAAGAAAAT 
GAAGTTTCTG AGGATGGAGT CTCGAGTACT GTGGACCAAC TTAGTGACAT TCATATCGAG 
ACGGGAGCCA ATGATTCTCA GCACTCTAAA TGTGATGTAG ATAAGTCTGT GCAACCAGAG 
CCATTTTTCC ATAAAGTGGT TCATTCTGAA CACTTGAACT TAGTCCCTCA AGTTCAATCA 
GTTCAGTGTT CACCAGAAGA ATCCTTTGCA TTTCGATCTC ACTCGCATTT ACCACCAAAA 
AATAAAAACA AGAATTCCTT GCTGATTGGA CTTTCAACTG GTCTGTTTGA TGCAAACAAC 
CCAAAGATGT TAAGGACATG TTCACTTCCA GATCTCTCAA AGCTGTTCAG AACCCTTATG 
GATGTTCCCA CCGTAGGAGA TGTTCGTCAA GACAATCTTG AAATAGATGA AATTGAAGAT 
GAAAACATTA AAGAAGGACC TTCTGATTCT GAAGACATTG TGTTTGAAGA AACTGACACA 
GATTTACAAG AGCTGCAGGC CTCAATGGAA CAGTTACTTA GGGAACAACC TAGTGAAGAA 
TACAGTGAAG AAGAAGAGTC AGTCTTAAAG AACAGTGATG TGGAGCCAAC TGCAAATGGG 
ACAGATGTGG CGGATGAAGA TGACAATCCC AGCAGTGAAA GTGCCCTGAA CGAAGAATGG 
CACTCAGATA ACAGTGATGG GGAAAATGCT AGTGAATGTG AATACGATAG TGTCTTTAAC 
CATTTAGAGG AACTGAGACT TCATCTGGAG CAGGAAATGG GCTTTGAAAA ATTCTTTGAG 
GTTTACGAGA AAATAAAGGC TATTCATGAA GATGAAGATG AAAATATTGA AATTTGTTCA 
AAAATAGTTC AAAATATTTT GGGAAATGAA CATCAGCATC TTTATGCCAA GATTCTTCAT 
TTAGTCATGG CAGATGGAGC CTACCAAGAA GATAATGATG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_028848881.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_028704714.1 Macaca mulatta NIMA related kinase 1 (NEK1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMb86669
Clone ID Related Accession (Same CDS sequence)	XM_028848881.1 , XM_028848880.1 , XM_028848879.1
Accession Version	XM_028848881.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3951bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-25
Organism	Macaca mulatta(Rhesus monkey)
Product	serine/threonine-protein kinase Nek1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041758.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Macaca mulatta Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG CAGACAGTAT GTTATCAAGG AAATTAACAT CTCAAGAATG 
TCCAGTAAAG AAAGAGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GTTCTCTCTA CATAGTGATG 
GATTACTGTG AGGGAGGGGA TCTGTTTAAG CGAATAAATG CTCAGAAAGG CGTTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTGAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATTAAA TCTCAGAACA TATTTTTAAC TAAAGATGGA 
ACGGTACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AATAATAAAA GTGACATTTG GGCTCTGGGG TGTGTCCTTT ATGAGCTGTG TACACTTAAA 
CATGCTTTTG AAGCTGGCAG TATGAAAAAC TTGGTACTGA AGATAATATC CGGATCTTTT 
CCACCTGTGT CTTTGCATTA TTCCTATGAT CTCCGCAGTT TGGTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC ACCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACGTTT 
TCAAAGTTTG GATCACAGCC TATACCAGCC AAAAGACCAG CTTCAGGACA AAACTCGATT 
TCTGTTATGC CTGCTCAGAA AATTACAAAG CCTGCCGCTA AATATGGAAT ACCTTTAACA 
TATAAGAAAT ATGGAGAGAA AAAATTATAC GAAAAGAAAC CACTGCAAAA ACATAAACAG 
GCCCATCAAA CTCCAGAGAA GAGAGTGAAT ATTGGAGAAG AAAGGAGGAA AATGTCTGAG 
GAAGCAGCAA GAAAGAGAAG GCTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTATTAGTT TAATGAAGGC TGAACAAATG AAAAGGCAAG AAAAGGAAAG GTTGGAAAGA 
ATAAATAGGG CCAGGGAACA AGGATGGAGA AATGTGCTAA GTGCTGGTGG AGGTGGTGAA 
GTAAAGGCTC CTTTTCTTGG CAGTGGAGGG ACTATAGCTC CATCATCTTT TTCTTCTCGA 
GGACAGTATG AACATTACCA TGCCATTTTT GACCAAATGC AACAACAGAG AGCAGAAGAT 
AATGAAGCTA AATGGAAAAG AGAAATATAT GGTCGAGGTC TTCCAGAAAG CGGAATTCTG 
CCTGGAGTTC GTCCAGGATT TCCTTATGGG GCTGCAGGTC ATCACCATTT CCCTGATGCT 
GATGATATTA GAAAAACTTT GAAAAGATTG AAGGCGGTGT CTAAACAAGC CAATGCAAAC 
AGGCAAAAAG GGCAGCTAGC TGTAGAAAGA GCTAAACAAG TAGAAGAGTT CCTGCAGCGA 
AAACGGGAAG CTATGCAGAA TAAAGCTCGA GCCGAAGGAC ATATGGGAAT CCTGCAAAAC 
CTGGCAGCTA TGTATGGAGG CAGGCCCAGC TCTTCAAGAG GAGGGAAGCC AAGGAACAAA 
GAGGAAGAGG TTTATCTGGC AAGACTGAGG CAAATAAGAC TACAGAATTT CAATGAACGC 
CAACAGATTA AAGCCAAACT TCGTGGTGAA AAGAAAGAAG CTAATCGTTC TGAAGGACAA 
GAAGGAAGTG AAGAGGCTGA CATGAGGCGC AAAAAAATCG AATCCCTGAA GGCCCATGCA 
AATGCACGTG CTGCTGTACT AAAAGAACAA CTAGAACGAA AGAGAAAGGA AGCTTATGAG 
AGAGAAAAAA AAGTGTGGGA AGAGCATTTG GTGGCTAAAG GAGTTAAGAG TTCTGATGTT 
TCTCCACCTT TGGGACAGCA TGAAACAGGT GGCTCTCCAT CAAAGCAACA GATGAGATCG 
GTTATTTCTG TGACTTCAGC TTTGAAAGAA GTTGGCGTGG ACAGTAGTTT AACTGATATC 
CGGGAAATTT CAGAAGAAAT GCAAAAGACC AACAATGCTA TTTCAAGTAA GCGAGAAATA 
CTTCGTAGAT TAAATGAAAA TCTTAAAGCT CAAGAAGATG AAAAAGGAAA GCAGCATCTC 
TCTGATACTT GTGAGATAAA TGTTCTTGAA GATGCCAAAG AGCATGAAAA AGAAAAATCA 
GTTTCATCTG ATCGCAAGAA GTGGGAGGCA GGAGGTCAAC TTGTGATTCC TCTGGATGAG 
TTAACACTAG ATACATCCTT CTCTACAACT GAAAGACATA CAGTGGGAGA AGTTATTAAA 
TTAGATCCTA ATGGATCTCC AAGAAGAGTC TGGGGGAAAA GTCCGACAGA TTCTGTTCTG 
AAGATACTTG GAGAAGCTGA ACTACAACTT CAGACAGAAC TATTAGAAAA TACAACTATT 
AGAAGTGATA TTTCTCCTGA AGGGGAAAAG TACAAACCCT TAATTACTGA AGAAAAAAAA 
GTGCAATGTA TTTCACATGA AATAAACCCA TCAGCTATTG TTGATTCTCC TGTTGAGACA 
AAAAGCCCCG AGTTTACTGA GGCATCTCCA CAGATGTCAT TGAAACTGGA AGGAAATTTA 
GAAGAACCTG ATGATTTGGA AACAGAAGTT CTACAAGAGC CAAGTGGAAC AAACAAAGAT 
GAGAGCTTGC CATGCACTGT TACTGATGTG TGGATTAGTG AGGAAAAAGA AGCAAAGGAA 
ACTCAGTTGG AAGATAGGAT CACCATTCAA GAAAATGAAG TTTCTGAGGA TGGAGTCTCG 
AGTACTGTGG ACCAACTTAG TGACATTCAT ATCGAGACGG GAGCCAATGA TTCTCAGCAC 
TCTAAATGTG ATGTAGATAA GTCTGTGCAA CCAGAGCCAT TTTTCCATAA AGTGGTTCAT 
TCTGAACACT TGAACTTAGT CCCTCAAGTT CAATCAGTTC AGTGTTCACC AGAAGAATCC 
TTTGCATTTC GATCTCACTC GCATTTACCA CCAAAAAATA AAAACAAGAA TTCCTTGCTG 
ATTGGACTTT CAACTGGTCT GTTTGATGCA AACAACCCAA AGATGTTAAG GACATGTTCA 
CTTCCAGATC TCTCAAAGCT GTTCAGAACC CTTATGGATG TTCCCACCGT AGGAGATGTT 
CGTCAAGACA ATCTTGAAAT AGATGAAATT GAAGATGAAA ACATTAAAGA AGGACCTTCT 
GATTCTGAAG ACATTGTGTT TGAAGAAACT GACACAGATT TACAAGAGCT GCAGGCCTCA 
ATGGAACAGT TACTTAGGGA ACAACCTAGT GAAGAATACA GTGAAGAAGA AGAGTCAGTC 
TTAAAGAACA GTGATGTGGA GCCAACTGCA AATGGGACAG ATGTGGCGGA TGAAGATGAC 
AATCCCAGCA GTGAAAGTGC CCTGAACGAA GAATGGCACT CAGATAACAG TGATGGGGAA 
AATGCTAGTG AATGTGAATA CGATAGTGTC TTTAACCATT TAGAGGAACT GAGACTTCAT 
CTGGAGCAGG AAATGGGCTT TGAAAAATTC TTTGAGGTTT ACGAGAAAAT AAAGGCTATT 
CATGAAGATG AAGATGAAAA TATTGAAATT TGTTCAAAAA TAGTTCAAAA TATTTTGGGA 
AATGAACATC AGCATCTTTA TGCCAAGATT CTTCATTTAG TCATGGCAGA TGGAGCCTAC 
CAAGAAGATG ATTGTCCTGC CAGACCTCTA TTGAGCTGGA TAGGGATGGC ACCATGTTCA 
AGAGGCCAAA GAAGAGACCT GGAGCCAGCA AACAGACACA AGGTTTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028704714.1
CDS	320..4270
Translation	MEKYVRLQKIGEGSFGKAILVKSTEDGRQYVIKEINISRMSSKEREESRREVAVLANMKHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGVLFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYELCTLKHAFEAGSMKNLVLKIISGSFPPVSLHYSYDLRSLVSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTFSKFGSQPIPAKRPASGQNSISVMPAQKITKPAAKYGIPLTYKKYGEKKLYEKKPLQKHKQAHQTPEKRVNIGEERRKMSEEAARKRRLEFIEKEKKQKDQIISLMKAEQMKRQEKERLERINRAREQGWRNVLSAGGGGEVKAPFLGSGGTIAPSSFSSRGQYEHYHAIFDQMQQQRAEDNEAKWKREIYGRGLPESGILPGVRPGFPYGAAGHHHFPDADDIRKTLKRLKAVSKQANANRQKGQLAVERAKQVEEFLQRKREAMQNKARAEGHMGILQNLAAMYGGRPSSSRGGKPRNKEEEVYLARLRQIRLQNFNERQQIKAKLRGEKKEANRSEGQEGSEEADMRRKKIESLKAHANARAAVLKEQLERKRKEAYEREKKVWEEHLVAKGVKSSDVSPPLGQHETGGSPSKQQMRSVISVTSALKEVGVDSSLTDIREISEEMQKTNNAISSKREILRRLNENLKAQEDEKGKQHLSDTCEINVLEDAKEHEKEKSVSSDRKKWEAGGQLVIPLDELTLDTSFSTTERHTVGEVIKLDPNGSPRRVWGKSPTDSVLKILGEAELQLQTELLENTTIRSDISPEGEKYKPLITEEKKVQCISHEINPSAIVDSPVETKSPEFTEASPQMSLKLEGNLEEPDDLETEVLQEPSGTNKDESLPCTVTDVWISEEKEAKETQLEDRITIQENEVSEDGVSSTVDQLSDIHIETGANDSQHSKCDVDKSVQPEPFFHKVVHSEHLNLVPQVQSVQCSPEESFAFRSHSHLPPKNKNKNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDVRQDNLEIDEIEDENIKEGPSDSEDIVFEETDTDLQELQASMEQLLREQPSEEYSEEEESVLKNSDVEPTANGTDVADEDDNPSSESALNEEWHSDNSDGENASECEYDSVFNHLEELRLHLEQEMGFEKFFEVYEKIKAIHEDEDENIEICSKIVQNILGNEHQHLYAKILHLVMADGAYQEDDCPARPLLSWIGMAPCSRGQRRDLEPANRHKVY

Target ORF information:

RefSeq Version	XM_028848881.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta NIMA related kinase 1 (NEK1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_028848881.1

ORF Insert Sequence:

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG CAGACAGTAT GTTATCAAGG AAATTAACAT CTCAAGAATG 
TCCAGTAAAG AAAGAGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GTTCTCTCTA CATAGTGATG 
GATTACTGTG AGGGAGGGGA TCTGTTTAAG CGAATAAATG CTCAGAAAGG CGTTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTGAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATTAAA TCTCAGAACA TATTTTTAAC TAAAGATGGA 
ACGGTACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AATAATAAAA GTGACATTTG GGCTCTGGGG TGTGTCCTTT ATGAGCTGTG TACACTTAAA 
CATGCTTTTG AAGCTGGCAG TATGAAAAAC TTGGTACTGA AGATAATATC CGGATCTTTT 
CCACCTGTGT CTTTGCATTA TTCCTATGAT CTCCGCAGTT TGGTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC ACCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACGTTT 
TCAAAGTTTG GATCACAGCC TATACCAGCC AAAAGACCAG CTTCAGGACA AAACTCGATT 
TCTGTTATGC CTGCTCAGAA AATTACAAAG CCTGCCGCTA AATATGGAAT ACCTTTAACA 
TATAAGAAAT ATGGAGAGAA AAAATTATAC GAAAAGAAAC CACTGCAAAA ACATAAACAG 
GCCCATCAAA CTCCAGAGAA GAGAGTGAAT ATTGGAGAAG AAAGGAGGAA AATGTCTGAG 
GAAGCAGCAA GAAAGAGAAG GCTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTATTAGTT TAATGAAGGC TGAACAAATG AAAAGGCAAG AAAAGGAAAG GTTGGAAAGA 
ATAAATAGGG CCAGGGAACA AGGATGGAGA AATGTGCTAA GTGCTGGTGG AGGTGGTGAA 
GTAAAGGCTC CTTTTCTTGG CAGTGGAGGG ACTATAGCTC CATCATCTTT TTCTTCTCGA 
GGACAGTATG AACATTACCA TGCCATTTTT GACCAAATGC AACAACAGAG AGCAGAAGAT 
AATGAAGCTA AATGGAAAAG AGAAATATAT GGTCGAGGTC TTCCAGAAAG CGGAATTCTG 
CCTGGAGTTC GTCCAGGATT TCCTTATGGG GCTGCAGGTC ATCACCATTT CCCTGATGCT 
GATGATATTA GAAAAACTTT GAAAAGATTG AAGGCGGTGT CTAAACAAGC CAATGCAAAC 
AGGCAAAAAG GGCAGCTAGC TGTAGAAAGA GCTAAACAAG TAGAAGAGTT CCTGCAGCGA 
AAACGGGAAG CTATGCAGAA TAAAGCTCGA GCCGAAGGAC ATATGGGAAT CCTGCAAAAC 
CTGGCAGCTA TGTATGGAGG CAGGCCCAGC TCTTCAAGAG GAGGGAAGCC AAGGAACAAA 
GAGGAAGAGG TTTATCTGGC AAGACTGAGG CAAATAAGAC TACAGAATTT CAATGAACGC 
CAACAGATTA AAGCCAAACT TCGTGGTGAA AAGAAAGAAG CTAATCGTTC TGAAGGACAA 
GAAGGAAGTG AAGAGGCTGA CATGAGGCGC AAAAAAATCG AATCCCTGAA GGCCCATGCA 
AATGCACGTG CTGCTGTACT AAAAGAACAA CTAGAACGAA AGAGAAAGGA AGCTTATGAG 
AGAGAAAAAA AAGTGTGGGA AGAGCATTTG GTGGCTAAAG GAGTTAAGAG TTCTGATGTT 
TCTCCACCTT TGGGACAGCA TGAAACAGGT GGCTCTCCAT CAAAGCAACA GATGAGATCG 
GTTATTTCTG TGACTTCAGC TTTGAAAGAA GTTGGCGTGG ACAGTAGTTT AACTGATATC 
CGGGAAATTT CAGAAGAAAT GCAAAAGACC AACAATGCTA TTTCAAGTAA GCGAGAAATA 
CTTCGTAGAT TAAATGAAAA TCTTAAAGCT CAAGAAGATG AAAAAGGAAA GCAGCATCTC 
TCTGATACTT GTGAGATAAA TGTTCTTGAA GATGCCAAAG AGCATGAAAA AGAAAAATCA 
GTTTCATCTG ATCGCAAGAA GTGGGAGGCA GGAGGTCAAC TTGTGATTCC TCTGGATGAG 
TTAACACTAG ATACATCCTT CTCTACAACT GAAAGACATA CAGTGGGAGA AGTTATTAAA 
TTAGATCCTA ATGGATCTCC AAGAAGAGTC TGGGGGAAAA GTCCGACAGA TTCTGTTCTG 
AAGATACTTG GAGAAGCTGA ACTACAACTT CAGACAGAAC TATTAGAAAA TACAACTATT 
AGAAGTGATA TTTCTCCTGA AGGGGAAAAG TACAAACCCT TAATTACTGA AGAAAAAAAA 
GTGCAATGTA TTTCACATGA AATAAACCCA TCAGCTATTG TTGATTCTCC TGTTGAGACA 
AAAAGCCCCG AGTTTACTGA GGCATCTCCA CAGATGTCAT TGAAACTGGA AGGAAATTTA 
GAAGAACCTG ATGATTTGGA AACAGAAGTT CTACAAGAGC CAAGTGGAAC AAACAAAGAT 
GAGAGCTTGC CATGCACTGT TACTGATGTG TGGATTAGTG AGGAAAAAGA AGCAAAGGAA 
ACTCAGTTGG AAGATAGGAT CACCATTCAA GAAAATGAAG TTTCTGAGGA TGGAGTCTCG 
AGTACTGTGG ACCAACTTAG TGACATTCAT ATCGAGACGG GAGCCAATGA TTCTCAGCAC 
TCTAAATGTG ATGTAGATAA GTCTGTGCAA CCAGAGCCAT TTTTCCATAA AGTGGTTCAT 
TCTGAACACT TGAACTTAGT CCCTCAAGTT CAATCAGTTC AGTGTTCACC AGAAGAATCC 
TTTGCATTTC GATCTCACTC GCATTTACCA CCAAAAAATA AAAACAAGAA TTCCTTGCTG 
ATTGGACTTT CAACTGGTCT GTTTGATGCA AACAACCCAA AGATGTTAAG GACATGTTCA 
CTTCCAGATC TCTCAAAGCT GTTCAGAACC CTTATGGATG TTCCCACCGT AGGAGATGTT 
CGTCAAGACA ATCTTGAAAT AGATGAAATT GAAGATGAAA ACATTAAAGA AGGACCTTCT 
GATTCTGAAG ACATTGTGTT TGAAGAAACT GACACAGATT TACAAGAGCT GCAGGCCTCA 
ATGGAACAGT TACTTAGGGA ACAACCTAGT GAAGAATACA GTGAAGAAGA AGAGTCAGTC 
TTAAAGAACA GTGATGTGGA GCCAACTGCA AATGGGACAG ATGTGGCGGA TGAAGATGAC 
AATCCCAGCA GTGAAAGTGC CCTGAACGAA GAATGGCACT CAGATAACAG TGATGGGGAA 
AATGCTAGTG AATGTGAATA CGATAGTGTC TTTAACCATT TAGAGGAACT GAGACTTCAT 
CTGGAGCAGG AAATGGGCTT TGAAAAATTC TTTGAGGTTT ACGAGAAAAT AAAGGCTATT 
CATGAAGATG AAGATGAAAA TATTGAAATT TGTTCAAAAA TAGTTCAAAA TATTTTGGGA 
AATGAACATC AGCATCTTTA TGCCAAGATT CTTCATTTAG TCATGGCAGA TGGAGCCTAC 
CAAGAAGATG ATTGTCCTGC CAGACCTCTA TTGAGCTGGA TAGGGATGGC ACCATGTTCA 
AGAGGCCAAA GAAGAGACCT GGAGCCAGCA AACAGACACA AGGTTTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_028848880.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_028704713.1 Macaca mulatta NIMA related kinase 1 (NEK1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMb86669
Clone ID Related Accession (Same CDS sequence)	XM_028848881.1 , XM_028848880.1 , XM_028848879.1
Accession Version	XM_028848880.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3951bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-25
Organism	Macaca mulatta(Rhesus monkey)
Product	serine/threonine-protein kinase Nek1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041758.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Macaca mulatta Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG CAGACAGTAT GTTATCAAGG AAATTAACAT CTCAAGAATG 
TCCAGTAAAG AAAGAGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GTTCTCTCTA CATAGTGATG 
GATTACTGTG AGGGAGGGGA TCTGTTTAAG CGAATAAATG CTCAGAAAGG CGTTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTGAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATTAAA TCTCAGAACA TATTTTTAAC TAAAGATGGA 
ACGGTACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AATAATAAAA GTGACATTTG GGCTCTGGGG TGTGTCCTTT ATGAGCTGTG TACACTTAAA 
CATGCTTTTG AAGCTGGCAG TATGAAAAAC TTGGTACTGA AGATAATATC CGGATCTTTT 
CCACCTGTGT CTTTGCATTA TTCCTATGAT CTCCGCAGTT TGGTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC ACCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACGTTT 
TCAAAGTTTG GATCACAGCC TATACCAGCC AAAAGACCAG CTTCAGGACA AAACTCGATT 
TCTGTTATGC CTGCTCAGAA AATTACAAAG CCTGCCGCTA AATATGGAAT ACCTTTAACA 
TATAAGAAAT ATGGAGAGAA AAAATTATAC GAAAAGAAAC CACTGCAAAA ACATAAACAG 
GCCCATCAAA CTCCAGAGAA GAGAGTGAAT ATTGGAGAAG AAAGGAGGAA AATGTCTGAG 
GAAGCAGCAA GAAAGAGAAG GCTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTATTAGTT TAATGAAGGC TGAACAAATG AAAAGGCAAG AAAAGGAAAG GTTGGAAAGA 
ATAAATAGGG CCAGGGAACA AGGATGGAGA AATGTGCTAA GTGCTGGTGG AGGTGGTGAA 
GTAAAGGCTC CTTTTCTTGG CAGTGGAGGG ACTATAGCTC CATCATCTTT TTCTTCTCGA 
GGACAGTATG AACATTACCA TGCCATTTTT GACCAAATGC AACAACAGAG AGCAGAAGAT 
AATGAAGCTA AATGGAAAAG AGAAATATAT GGTCGAGGTC TTCCAGAAAG CGGAATTCTG 
CCTGGAGTTC GTCCAGGATT TCCTTATGGG GCTGCAGGTC ATCACCATTT CCCTGATGCT 
GATGATATTA GAAAAACTTT GAAAAGATTG AAGGCGGTGT CTAAACAAGC CAATGCAAAC 
AGGCAAAAAG GGCAGCTAGC TGTAGAAAGA GCTAAACAAG TAGAAGAGTT CCTGCAGCGA 
AAACGGGAAG CTATGCAGAA TAAAGCTCGA GCCGAAGGAC ATATGGGAAT CCTGCAAAAC 
CTGGCAGCTA TGTATGGAGG CAGGCCCAGC TCTTCAAGAG GAGGGAAGCC AAGGAACAAA 
GAGGAAGAGG TTTATCTGGC AAGACTGAGG CAAATAAGAC TACAGAATTT CAATGAACGC 
CAACAGATTA AAGCCAAACT TCGTGGTGAA AAGAAAGAAG CTAATCGTTC TGAAGGACAA 
GAAGGAAGTG AAGAGGCTGA CATGAGGCGC AAAAAAATCG AATCCCTGAA GGCCCATGCA 
AATGCACGTG CTGCTGTACT AAAAGAACAA CTAGAACGAA AGAGAAAGGA AGCTTATGAG 
AGAGAAAAAA AAGTGTGGGA AGAGCATTTG GTGGCTAAAG GAGTTAAGAG TTCTGATGTT 
TCTCCACCTT TGGGACAGCA TGAAACAGGT GGCTCTCCAT CAAAGCAACA GATGAGATCG 
GTTATTTCTG TGACTTCAGC TTTGAAAGAA GTTGGCGTGG ACAGTAGTTT AACTGATATC 
CGGGAAATTT CAGAAGAAAT GCAAAAGACC AACAATGCTA TTTCAAGTAA GCGAGAAATA 
CTTCGTAGAT TAAATGAAAA TCTTAAAGCT CAAGAAGATG AAAAAGGAAA GCAGCATCTC 
TCTGATACTT GTGAGATAAA TGTTCTTGAA GATGCCAAAG AGCATGAAAA AGAAAAATCA 
GTTTCATCTG ATCGCAAGAA GTGGGAGGCA GGAGGTCAAC TTGTGATTCC TCTGGATGAG 
TTAACACTAG ATACATCCTT CTCTACAACT GAAAGACATA CAGTGGGAGA AGTTATTAAA 
TTAGATCCTA ATGGATCTCC AAGAAGAGTC TGGGGGAAAA GTCCGACAGA TTCTGTTCTG 
AAGATACTTG GAGAAGCTGA ACTACAACTT CAGACAGAAC TATTAGAAAA TACAACTATT 
AGAAGTGATA TTTCTCCTGA AGGGGAAAAG TACAAACCCT TAATTACTGA AGAAAAAAAA 
GTGCAATGTA TTTCACATGA AATAAACCCA TCAGCTATTG TTGATTCTCC TGTTGAGACA 
AAAAGCCCCG AGTTTACTGA GGCATCTCCA CAGATGTCAT TGAAACTGGA AGGAAATTTA 
GAAGAACCTG ATGATTTGGA AACAGAAGTT CTACAAGAGC CAAGTGGAAC AAACAAAGAT 
GAGAGCTTGC CATGCACTGT TACTGATGTG TGGATTAGTG AGGAAAAAGA AGCAAAGGAA 
ACTCAGTTGG AAGATAGGAT CACCATTCAA GAAAATGAAG TTTCTGAGGA TGGAGTCTCG 
AGTACTGTGG ACCAACTTAG TGACATTCAT ATCGAGACGG GAGCCAATGA TTCTCAGCAC 
TCTAAATGTG ATGTAGATAA GTCTGTGCAA CCAGAGCCAT TTTTCCATAA AGTGGTTCAT 
TCTGAACACT TGAACTTAGT CCCTCAAGTT CAATCAGTTC AGTGTTCACC AGAAGAATCC 
TTTGCATTTC GATCTCACTC GCATTTACCA CCAAAAAATA AAAACAAGAA TTCCTTGCTG 
ATTGGACTTT CAACTGGTCT GTTTGATGCA AACAACCCAA AGATGTTAAG GACATGTTCA 
CTTCCAGATC TCTCAAAGCT GTTCAGAACC CTTATGGATG TTCCCACCGT AGGAGATGTT 
CGTCAAGACA ATCTTGAAAT AGATGAAATT GAAGATGAAA ACATTAAAGA AGGACCTTCT 
GATTCTGAAG ACATTGTGTT TGAAGAAACT GACACAGATT TACAAGAGCT GCAGGCCTCA 
ATGGAACAGT TACTTAGGGA ACAACCTAGT GAAGAATACA GTGAAGAAGA AGAGTCAGTC 
TTAAAGAACA GTGATGTGGA GCCAACTGCA AATGGGACAG ATGTGGCGGA TGAAGATGAC 
AATCCCAGCA GTGAAAGTGC CCTGAACGAA GAATGGCACT CAGATAACAG TGATGGGGAA 
AATGCTAGTG AATGTGAATA CGATAGTGTC TTTAACCATT TAGAGGAACT GAGACTTCAT 
CTGGAGCAGG AAATGGGCTT TGAAAAATTC TTTGAGGTTT ACGAGAAAAT AAAGGCTATT 
CATGAAGATG AAGATGAAAA TATTGAAATT TGTTCAAAAA TAGTTCAAAA TATTTTGGGA 
AATGAACATC AGCATCTTTA TGCCAAGATT CTTCATTTAG TCATGGCAGA TGGAGCCTAC 
CAAGAAGATG ATTGTCCTGC CAGACCTCTA TTGAGCTGGA TAGGGATGGC ACCATGTTCA 
AGAGGCCAAA GAAGAGACCT GGAGCCAGCA AACAGACACA AGGTTTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028704713.1
CDS	575..4525
Translation	MEKYVRLQKIGEGSFGKAILVKSTEDGRQYVIKEINISRMSSKEREESRREVAVLANMKHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGVLFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYELCTLKHAFEAGSMKNLVLKIISGSFPPVSLHYSYDLRSLVSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTFSKFGSQPIPAKRPASGQNSISVMPAQKITKPAAKYGIPLTYKKYGEKKLYEKKPLQKHKQAHQTPEKRVNIGEERRKMSEEAARKRRLEFIEKEKKQKDQIISLMKAEQMKRQEKERLERINRAREQGWRNVLSAGGGGEVKAPFLGSGGTIAPSSFSSRGQYEHYHAIFDQMQQQRAEDNEAKWKREIYGRGLPESGILPGVRPGFPYGAAGHHHFPDADDIRKTLKRLKAVSKQANANRQKGQLAVERAKQVEEFLQRKREAMQNKARAEGHMGILQNLAAMYGGRPSSSRGGKPRNKEEEVYLARLRQIRLQNFNERQQIKAKLRGEKKEANRSEGQEGSEEADMRRKKIESLKAHANARAAVLKEQLERKRKEAYEREKKVWEEHLVAKGVKSSDVSPPLGQHETGGSPSKQQMRSVISVTSALKEVGVDSSLTDIREISEEMQKTNNAISSKREILRRLNENLKAQEDEKGKQHLSDTCEINVLEDAKEHEKEKSVSSDRKKWEAGGQLVIPLDELTLDTSFSTTERHTVGEVIKLDPNGSPRRVWGKSPTDSVLKILGEAELQLQTELLENTTIRSDISPEGEKYKPLITEEKKVQCISHEINPSAIVDSPVETKSPEFTEASPQMSLKLEGNLEEPDDLETEVLQEPSGTNKDESLPCTVTDVWISEEKEAKETQLEDRITIQENEVSEDGVSSTVDQLSDIHIETGANDSQHSKCDVDKSVQPEPFFHKVVHSEHLNLVPQVQSVQCSPEESFAFRSHSHLPPKNKNKNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDVRQDNLEIDEIEDENIKEGPSDSEDIVFEETDTDLQELQASMEQLLREQPSEEYSEEEESVLKNSDVEPTANGTDVADEDDNPSSESALNEEWHSDNSDGENASECEYDSVFNHLEELRLHLEQEMGFEKFFEVYEKIKAIHEDEDENIEICSKIVQNILGNEHQHLYAKILHLVMADGAYQEDDCPARPLLSWIGMAPCSRGQRRDLEPANRHKVY

Target ORF information:

RefSeq Version	XM_028848880.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta NIMA related kinase 1 (NEK1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_028848880.1

ORF Insert Sequence:

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG CAGACAGTAT GTTATCAAGG AAATTAACAT CTCAAGAATG 
TCCAGTAAAG AAAGAGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GTTCTCTCTA CATAGTGATG 
GATTACTGTG AGGGAGGGGA TCTGTTTAAG CGAATAAATG CTCAGAAAGG CGTTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTGAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATTAAA TCTCAGAACA TATTTTTAAC TAAAGATGGA 
ACGGTACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AATAATAAAA GTGACATTTG GGCTCTGGGG TGTGTCCTTT ATGAGCTGTG TACACTTAAA 
CATGCTTTTG AAGCTGGCAG TATGAAAAAC TTGGTACTGA AGATAATATC CGGATCTTTT 
CCACCTGTGT CTTTGCATTA TTCCTATGAT CTCCGCAGTT TGGTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC ACCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACGTTT 
TCAAAGTTTG GATCACAGCC TATACCAGCC AAAAGACCAG CTTCAGGACA AAACTCGATT 
TCTGTTATGC CTGCTCAGAA AATTACAAAG CCTGCCGCTA AATATGGAAT ACCTTTAACA 
TATAAGAAAT ATGGAGAGAA AAAATTATAC GAAAAGAAAC CACTGCAAAA ACATAAACAG 
GCCCATCAAA CTCCAGAGAA GAGAGTGAAT ATTGGAGAAG AAAGGAGGAA AATGTCTGAG 
GAAGCAGCAA GAAAGAGAAG GCTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTATTAGTT TAATGAAGGC TGAACAAATG AAAAGGCAAG AAAAGGAAAG GTTGGAAAGA 
ATAAATAGGG CCAGGGAACA AGGATGGAGA AATGTGCTAA GTGCTGGTGG AGGTGGTGAA 
GTAAAGGCTC CTTTTCTTGG CAGTGGAGGG ACTATAGCTC CATCATCTTT TTCTTCTCGA 
GGACAGTATG AACATTACCA TGCCATTTTT GACCAAATGC AACAACAGAG AGCAGAAGAT 
AATGAAGCTA AATGGAAAAG AGAAATATAT GGTCGAGGTC TTCCAGAAAG CGGAATTCTG 
CCTGGAGTTC GTCCAGGATT TCCTTATGGG GCTGCAGGTC ATCACCATTT CCCTGATGCT 
GATGATATTA GAAAAACTTT GAAAAGATTG AAGGCGGTGT CTAAACAAGC CAATGCAAAC 
AGGCAAAAAG GGCAGCTAGC TGTAGAAAGA GCTAAACAAG TAGAAGAGTT CCTGCAGCGA 
AAACGGGAAG CTATGCAGAA TAAAGCTCGA GCCGAAGGAC ATATGGGAAT CCTGCAAAAC 
CTGGCAGCTA TGTATGGAGG CAGGCCCAGC TCTTCAAGAG GAGGGAAGCC AAGGAACAAA 
GAGGAAGAGG TTTATCTGGC AAGACTGAGG CAAATAAGAC TACAGAATTT CAATGAACGC 
CAACAGATTA AAGCCAAACT TCGTGGTGAA AAGAAAGAAG CTAATCGTTC TGAAGGACAA 
GAAGGAAGTG AAGAGGCTGA CATGAGGCGC AAAAAAATCG AATCCCTGAA GGCCCATGCA 
AATGCACGTG CTGCTGTACT AAAAGAACAA CTAGAACGAA AGAGAAAGGA AGCTTATGAG 
AGAGAAAAAA AAGTGTGGGA AGAGCATTTG GTGGCTAAAG GAGTTAAGAG TTCTGATGTT 
TCTCCACCTT TGGGACAGCA TGAAACAGGT GGCTCTCCAT CAAAGCAACA GATGAGATCG 
GTTATTTCTG TGACTTCAGC TTTGAAAGAA GTTGGCGTGG ACAGTAGTTT AACTGATATC 
CGGGAAATTT CAGAAGAAAT GCAAAAGACC AACAATGCTA TTTCAAGTAA GCGAGAAATA 
CTTCGTAGAT TAAATGAAAA TCTTAAAGCT CAAGAAGATG AAAAAGGAAA GCAGCATCTC 
TCTGATACTT GTGAGATAAA TGTTCTTGAA GATGCCAAAG AGCATGAAAA AGAAAAATCA 
GTTTCATCTG ATCGCAAGAA GTGGGAGGCA GGAGGTCAAC TTGTGATTCC TCTGGATGAG 
TTAACACTAG ATACATCCTT CTCTACAACT GAAAGACATA CAGTGGGAGA AGTTATTAAA 
TTAGATCCTA ATGGATCTCC AAGAAGAGTC TGGGGGAAAA GTCCGACAGA TTCTGTTCTG 
AAGATACTTG GAGAAGCTGA ACTACAACTT CAGACAGAAC TATTAGAAAA TACAACTATT 
AGAAGTGATA TTTCTCCTGA AGGGGAAAAG TACAAACCCT TAATTACTGA AGAAAAAAAA 
GTGCAATGTA TTTCACATGA AATAAACCCA TCAGCTATTG TTGATTCTCC TGTTGAGACA 
AAAAGCCCCG AGTTTACTGA GGCATCTCCA CAGATGTCAT TGAAACTGGA AGGAAATTTA 
GAAGAACCTG ATGATTTGGA AACAGAAGTT CTACAAGAGC CAAGTGGAAC AAACAAAGAT 
GAGAGCTTGC CATGCACTGT TACTGATGTG TGGATTAGTG AGGAAAAAGA AGCAAAGGAA 
ACTCAGTTGG AAGATAGGAT CACCATTCAA GAAAATGAAG TTTCTGAGGA TGGAGTCTCG 
AGTACTGTGG ACCAACTTAG TGACATTCAT ATCGAGACGG GAGCCAATGA TTCTCAGCAC 
TCTAAATGTG ATGTAGATAA GTCTGTGCAA CCAGAGCCAT TTTTCCATAA AGTGGTTCAT 
TCTGAACACT TGAACTTAGT CCCTCAAGTT CAATCAGTTC AGTGTTCACC AGAAGAATCC 
TTTGCATTTC GATCTCACTC GCATTTACCA CCAAAAAATA AAAACAAGAA TTCCTTGCTG 
ATTGGACTTT CAACTGGTCT GTTTGATGCA AACAACCCAA AGATGTTAAG GACATGTTCA 
CTTCCAGATC TCTCAAAGCT GTTCAGAACC CTTATGGATG TTCCCACCGT AGGAGATGTT 
CGTCAAGACA ATCTTGAAAT AGATGAAATT GAAGATGAAA ACATTAAAGA AGGACCTTCT 
GATTCTGAAG ACATTGTGTT TGAAGAAACT GACACAGATT TACAAGAGCT GCAGGCCTCA 
ATGGAACAGT TACTTAGGGA ACAACCTAGT GAAGAATACA GTGAAGAAGA AGAGTCAGTC 
TTAAAGAACA GTGATGTGGA GCCAACTGCA AATGGGACAG ATGTGGCGGA TGAAGATGAC 
AATCCCAGCA GTGAAAGTGC CCTGAACGAA GAATGGCACT CAGATAACAG TGATGGGGAA 
AATGCTAGTG AATGTGAATA CGATAGTGTC TTTAACCATT TAGAGGAACT GAGACTTCAT 
CTGGAGCAGG AAATGGGCTT TGAAAAATTC TTTGAGGTTT ACGAGAAAAT AAAGGCTATT 
CATGAAGATG AAGATGAAAA TATTGAAATT TGTTCAAAAA TAGTTCAAAA TATTTTGGGA 
AATGAACATC AGCATCTTTA TGCCAAGATT CTTCATTTAG TCATGGCAGA TGGAGCCTAC 
CAAGAAGATG ATTGTCCTGC CAGACCTCTA TTGAGCTGGA TAGGGATGGC ACCATGTTCA 
AGAGGCCAAA GAAGAGACCT GGAGCCAGCA AACAGACACA AGGTTTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_028848879.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_028704712.1 Macaca mulatta NIMA related kinase 1 (NEK1), transcript variant X1, mRNA.

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CloneID	OMb86669
Clone ID Related Accession (Same CDS sequence)	XM_028848881.1 , XM_028848880.1 , XM_028848879.1
Accession Version	XM_028848879.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3951bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-25
Organism	Macaca mulatta(Rhesus monkey)
Product	serine/threonine-protein kinase Nek1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041758.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Macaca mulatta Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG CAGACAGTAT GTTATCAAGG AAATTAACAT CTCAAGAATG 
TCCAGTAAAG AAAGAGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GTTCTCTCTA CATAGTGATG 
GATTACTGTG AGGGAGGGGA TCTGTTTAAG CGAATAAATG CTCAGAAAGG CGTTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTGAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATTAAA TCTCAGAACA TATTTTTAAC TAAAGATGGA 
ACGGTACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AATAATAAAA GTGACATTTG GGCTCTGGGG TGTGTCCTTT ATGAGCTGTG TACACTTAAA 
CATGCTTTTG AAGCTGGCAG TATGAAAAAC TTGGTACTGA AGATAATATC CGGATCTTTT 
CCACCTGTGT CTTTGCATTA TTCCTATGAT CTCCGCAGTT TGGTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC ACCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACGTTT 
TCAAAGTTTG GATCACAGCC TATACCAGCC AAAAGACCAG CTTCAGGACA AAACTCGATT 
TCTGTTATGC CTGCTCAGAA AATTACAAAG CCTGCCGCTA AATATGGAAT ACCTTTAACA 
TATAAGAAAT ATGGAGAGAA AAAATTATAC GAAAAGAAAC CACTGCAAAA ACATAAACAG 
GCCCATCAAA CTCCAGAGAA GAGAGTGAAT ATTGGAGAAG AAAGGAGGAA AATGTCTGAG 
GAAGCAGCAA GAAAGAGAAG GCTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTATTAGTT TAATGAAGGC TGAACAAATG AAAAGGCAAG AAAAGGAAAG GTTGGAAAGA 
ATAAATAGGG CCAGGGAACA AGGATGGAGA AATGTGCTAA GTGCTGGTGG AGGTGGTGAA 
GTAAAGGCTC CTTTTCTTGG CAGTGGAGGG ACTATAGCTC CATCATCTTT TTCTTCTCGA 
GGACAGTATG AACATTACCA TGCCATTTTT GACCAAATGC AACAACAGAG AGCAGAAGAT 
AATGAAGCTA AATGGAAAAG AGAAATATAT GGTCGAGGTC TTCCAGAAAG CGGAATTCTG 
CCTGGAGTTC GTCCAGGATT TCCTTATGGG GCTGCAGGTC ATCACCATTT CCCTGATGCT 
GATGATATTA GAAAAACTTT GAAAAGATTG AAGGCGGTGT CTAAACAAGC CAATGCAAAC 
AGGCAAAAAG GGCAGCTAGC TGTAGAAAGA GCTAAACAAG TAGAAGAGTT CCTGCAGCGA 
AAACGGGAAG CTATGCAGAA TAAAGCTCGA GCCGAAGGAC ATATGGGAAT CCTGCAAAAC 
CTGGCAGCTA TGTATGGAGG CAGGCCCAGC TCTTCAAGAG GAGGGAAGCC AAGGAACAAA 
GAGGAAGAGG TTTATCTGGC AAGACTGAGG CAAATAAGAC TACAGAATTT CAATGAACGC 
CAACAGATTA AAGCCAAACT TCGTGGTGAA AAGAAAGAAG CTAATCGTTC TGAAGGACAA 
GAAGGAAGTG AAGAGGCTGA CATGAGGCGC AAAAAAATCG AATCCCTGAA GGCCCATGCA 
AATGCACGTG CTGCTGTACT AAAAGAACAA CTAGAACGAA AGAGAAAGGA AGCTTATGAG 
AGAGAAAAAA AAGTGTGGGA AGAGCATTTG GTGGCTAAAG GAGTTAAGAG TTCTGATGTT 
TCTCCACCTT TGGGACAGCA TGAAACAGGT GGCTCTCCAT CAAAGCAACA GATGAGATCG 
GTTATTTCTG TGACTTCAGC TTTGAAAGAA GTTGGCGTGG ACAGTAGTTT AACTGATATC 
CGGGAAATTT CAGAAGAAAT GCAAAAGACC AACAATGCTA TTTCAAGTAA GCGAGAAATA 
CTTCGTAGAT TAAATGAAAA TCTTAAAGCT CAAGAAGATG AAAAAGGAAA GCAGCATCTC 
TCTGATACTT GTGAGATAAA TGTTCTTGAA GATGCCAAAG AGCATGAAAA AGAAAAATCA 
GTTTCATCTG ATCGCAAGAA GTGGGAGGCA GGAGGTCAAC TTGTGATTCC TCTGGATGAG 
TTAACACTAG ATACATCCTT CTCTACAACT GAAAGACATA CAGTGGGAGA AGTTATTAAA 
TTAGATCCTA ATGGATCTCC AAGAAGAGTC TGGGGGAAAA GTCCGACAGA TTCTGTTCTG 
AAGATACTTG GAGAAGCTGA ACTACAACTT CAGACAGAAC TATTAGAAAA TACAACTATT 
AGAAGTGATA TTTCTCCTGA AGGGGAAAAG TACAAACCCT TAATTACTGA AGAAAAAAAA 
GTGCAATGTA TTTCACATGA AATAAACCCA TCAGCTATTG TTGATTCTCC TGTTGAGACA 
AAAAGCCCCG AGTTTACTGA GGCATCTCCA CAGATGTCAT TGAAACTGGA AGGAAATTTA 
GAAGAACCTG ATGATTTGGA AACAGAAGTT CTACAAGAGC CAAGTGGAAC AAACAAAGAT 
GAGAGCTTGC CATGCACTGT TACTGATGTG TGGATTAGTG AGGAAAAAGA AGCAAAGGAA 
ACTCAGTTGG AAGATAGGAT CACCATTCAA GAAAATGAAG TTTCTGAGGA TGGAGTCTCG 
AGTACTGTGG ACCAACTTAG TGACATTCAT ATCGAGACGG GAGCCAATGA TTCTCAGCAC 
TCTAAATGTG ATGTAGATAA GTCTGTGCAA CCAGAGCCAT TTTTCCATAA AGTGGTTCAT 
TCTGAACACT TGAACTTAGT CCCTCAAGTT CAATCAGTTC AGTGTTCACC AGAAGAATCC 
TTTGCATTTC GATCTCACTC GCATTTACCA CCAAAAAATA AAAACAAGAA TTCCTTGCTG 
ATTGGACTTT CAACTGGTCT GTTTGATGCA AACAACCCAA AGATGTTAAG GACATGTTCA 
CTTCCAGATC TCTCAAAGCT GTTCAGAACC CTTATGGATG TTCCCACCGT AGGAGATGTT 
CGTCAAGACA ATCTTGAAAT AGATGAAATT GAAGATGAAA ACATTAAAGA AGGACCTTCT 
GATTCTGAAG ACATTGTGTT TGAAGAAACT GACACAGATT TACAAGAGCT GCAGGCCTCA 
ATGGAACAGT TACTTAGGGA ACAACCTAGT GAAGAATACA GTGAAGAAGA AGAGTCAGTC 
TTAAAGAACA GTGATGTGGA GCCAACTGCA AATGGGACAG ATGTGGCGGA TGAAGATGAC 
AATCCCAGCA GTGAAAGTGC CCTGAACGAA GAATGGCACT CAGATAACAG TGATGGGGAA 
AATGCTAGTG AATGTGAATA CGATAGTGTC TTTAACCATT TAGAGGAACT GAGACTTCAT 
CTGGAGCAGG AAATGGGCTT TGAAAAATTC TTTGAGGTTT ACGAGAAAAT AAAGGCTATT 
CATGAAGATG AAGATGAAAA TATTGAAATT TGTTCAAAAA TAGTTCAAAA TATTTTGGGA 
AATGAACATC AGCATCTTTA TGCCAAGATT CTTCATTTAG TCATGGCAGA TGGAGCCTAC 
CAAGAAGATG ATTGTCCTGC CAGACCTCTA TTGAGCTGGA TAGGGATGGC ACCATGTTCA 
AGAGGCCAAA GAAGAGACCT GGAGCCAGCA AACAGACACA AGGTTTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028704712.1
CDS	544..4494
Translation	MEKYVRLQKIGEGSFGKAILVKSTEDGRQYVIKEINISRMSSKEREESRREVAVLANMKHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGVLFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYELCTLKHAFEAGSMKNLVLKIISGSFPPVSLHYSYDLRSLVSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTFSKFGSQPIPAKRPASGQNSISVMPAQKITKPAAKYGIPLTYKKYGEKKLYEKKPLQKHKQAHQTPEKRVNIGEERRKMSEEAARKRRLEFIEKEKKQKDQIISLMKAEQMKRQEKERLERINRAREQGWRNVLSAGGGGEVKAPFLGSGGTIAPSSFSSRGQYEHYHAIFDQMQQQRAEDNEAKWKREIYGRGLPESGILPGVRPGFPYGAAGHHHFPDADDIRKTLKRLKAVSKQANANRQKGQLAVERAKQVEEFLQRKREAMQNKARAEGHMGILQNLAAMYGGRPSSSRGGKPRNKEEEVYLARLRQIRLQNFNERQQIKAKLRGEKKEANRSEGQEGSEEADMRRKKIESLKAHANARAAVLKEQLERKRKEAYEREKKVWEEHLVAKGVKSSDVSPPLGQHETGGSPSKQQMRSVISVTSALKEVGVDSSLTDIREISEEMQKTNNAISSKREILRRLNENLKAQEDEKGKQHLSDTCEINVLEDAKEHEKEKSVSSDRKKWEAGGQLVIPLDELTLDTSFSTTERHTVGEVIKLDPNGSPRRVWGKSPTDSVLKILGEAELQLQTELLENTTIRSDISPEGEKYKPLITEEKKVQCISHEINPSAIVDSPVETKSPEFTEASPQMSLKLEGNLEEPDDLETEVLQEPSGTNKDESLPCTVTDVWISEEKEAKETQLEDRITIQENEVSEDGVSSTVDQLSDIHIETGANDSQHSKCDVDKSVQPEPFFHKVVHSEHLNLVPQVQSVQCSPEESFAFRSHSHLPPKNKNKNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDVRQDNLEIDEIEDENIKEGPSDSEDIVFEETDTDLQELQASMEQLLREQPSEEYSEEEESVLKNSDVEPTANGTDVADEDDNPSSESALNEEWHSDNSDGENASECEYDSVFNHLEELRLHLEQEMGFEKFFEVYEKIKAIHEDEDENIEICSKIVQNILGNEHQHLYAKILHLVMADGAYQEDDCPARPLLSWIGMAPCSRGQRRDLEPANRHKVY

Target ORF information:

RefSeq Version	XM_028848879.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta NIMA related kinase 1 (NEK1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_028848879.1

ORF Insert Sequence:

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG CAGACAGTAT GTTATCAAGG AAATTAACAT CTCAAGAATG 
TCCAGTAAAG AAAGAGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GTTCTCTCTA CATAGTGATG 
GATTACTGTG AGGGAGGGGA TCTGTTTAAG CGAATAAATG CTCAGAAAGG CGTTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTGAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATTAAA TCTCAGAACA TATTTTTAAC TAAAGATGGA 
ACGGTACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AATAATAAAA GTGACATTTG GGCTCTGGGG TGTGTCCTTT ATGAGCTGTG TACACTTAAA 
CATGCTTTTG AAGCTGGCAG TATGAAAAAC TTGGTACTGA AGATAATATC CGGATCTTTT 
CCACCTGTGT CTTTGCATTA TTCCTATGAT CTCCGCAGTT TGGTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC ACCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACGTTT 
TCAAAGTTTG GATCACAGCC TATACCAGCC AAAAGACCAG CTTCAGGACA AAACTCGATT 
TCTGTTATGC CTGCTCAGAA AATTACAAAG CCTGCCGCTA AATATGGAAT ACCTTTAACA 
TATAAGAAAT ATGGAGAGAA AAAATTATAC GAAAAGAAAC CACTGCAAAA ACATAAACAG 
GCCCATCAAA CTCCAGAGAA GAGAGTGAAT ATTGGAGAAG AAAGGAGGAA AATGTCTGAG 
GAAGCAGCAA GAAAGAGAAG GCTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTATTAGTT TAATGAAGGC TGAACAAATG AAAAGGCAAG AAAAGGAAAG GTTGGAAAGA 
ATAAATAGGG CCAGGGAACA AGGATGGAGA AATGTGCTAA GTGCTGGTGG AGGTGGTGAA 
GTAAAGGCTC CTTTTCTTGG CAGTGGAGGG ACTATAGCTC CATCATCTTT TTCTTCTCGA 
GGACAGTATG AACATTACCA TGCCATTTTT GACCAAATGC AACAACAGAG AGCAGAAGAT 
AATGAAGCTA AATGGAAAAG AGAAATATAT GGTCGAGGTC TTCCAGAAAG CGGAATTCTG 
CCTGGAGTTC GTCCAGGATT TCCTTATGGG GCTGCAGGTC ATCACCATTT CCCTGATGCT 
GATGATATTA GAAAAACTTT GAAAAGATTG AAGGCGGTGT CTAAACAAGC CAATGCAAAC 
AGGCAAAAAG GGCAGCTAGC TGTAGAAAGA GCTAAACAAG TAGAAGAGTT CCTGCAGCGA 
AAACGGGAAG CTATGCAGAA TAAAGCTCGA GCCGAAGGAC ATATGGGAAT CCTGCAAAAC 
CTGGCAGCTA TGTATGGAGG CAGGCCCAGC TCTTCAAGAG GAGGGAAGCC AAGGAACAAA 
GAGGAAGAGG TTTATCTGGC AAGACTGAGG CAAATAAGAC TACAGAATTT CAATGAACGC 
CAACAGATTA AAGCCAAACT TCGTGGTGAA AAGAAAGAAG CTAATCGTTC TGAAGGACAA 
GAAGGAAGTG AAGAGGCTGA CATGAGGCGC AAAAAAATCG AATCCCTGAA GGCCCATGCA 
AATGCACGTG CTGCTGTACT AAAAGAACAA CTAGAACGAA AGAGAAAGGA AGCTTATGAG 
AGAGAAAAAA AAGTGTGGGA AGAGCATTTG GTGGCTAAAG GAGTTAAGAG TTCTGATGTT 
TCTCCACCTT TGGGACAGCA TGAAACAGGT GGCTCTCCAT CAAAGCAACA GATGAGATCG 
GTTATTTCTG TGACTTCAGC TTTGAAAGAA GTTGGCGTGG ACAGTAGTTT AACTGATATC 
CGGGAAATTT CAGAAGAAAT GCAAAAGACC AACAATGCTA TTTCAAGTAA GCGAGAAATA 
CTTCGTAGAT TAAATGAAAA TCTTAAAGCT CAAGAAGATG AAAAAGGAAA GCAGCATCTC 
TCTGATACTT GTGAGATAAA TGTTCTTGAA GATGCCAAAG AGCATGAAAA AGAAAAATCA 
GTTTCATCTG ATCGCAAGAA GTGGGAGGCA GGAGGTCAAC TTGTGATTCC TCTGGATGAG 
TTAACACTAG ATACATCCTT CTCTACAACT GAAAGACATA CAGTGGGAGA AGTTATTAAA 
TTAGATCCTA ATGGATCTCC AAGAAGAGTC TGGGGGAAAA GTCCGACAGA TTCTGTTCTG 
AAGATACTTG GAGAAGCTGA ACTACAACTT CAGACAGAAC TATTAGAAAA TACAACTATT 
AGAAGTGATA TTTCTCCTGA AGGGGAAAAG TACAAACCCT TAATTACTGA AGAAAAAAAA 
GTGCAATGTA TTTCACATGA AATAAACCCA TCAGCTATTG TTGATTCTCC TGTTGAGACA 
AAAAGCCCCG AGTTTACTGA GGCATCTCCA CAGATGTCAT TGAAACTGGA AGGAAATTTA 
GAAGAACCTG ATGATTTGGA AACAGAAGTT CTACAAGAGC CAAGTGGAAC AAACAAAGAT 
GAGAGCTTGC CATGCACTGT TACTGATGTG TGGATTAGTG AGGAAAAAGA AGCAAAGGAA 
ACTCAGTTGG AAGATAGGAT CACCATTCAA GAAAATGAAG TTTCTGAGGA TGGAGTCTCG 
AGTACTGTGG ACCAACTTAG TGACATTCAT ATCGAGACGG GAGCCAATGA TTCTCAGCAC 
TCTAAATGTG ATGTAGATAA GTCTGTGCAA CCAGAGCCAT TTTTCCATAA AGTGGTTCAT 
TCTGAACACT TGAACTTAGT CCCTCAAGTT CAATCAGTTC AGTGTTCACC AGAAGAATCC 
TTTGCATTTC GATCTCACTC GCATTTACCA CCAAAAAATA AAAACAAGAA TTCCTTGCTG 
ATTGGACTTT CAACTGGTCT GTTTGATGCA AACAACCCAA AGATGTTAAG GACATGTTCA 
CTTCCAGATC TCTCAAAGCT GTTCAGAACC CTTATGGATG TTCCCACCGT AGGAGATGTT 
CGTCAAGACA ATCTTGAAAT AGATGAAATT GAAGATGAAA ACATTAAAGA AGGACCTTCT 
GATTCTGAAG ACATTGTGTT TGAAGAAACT GACACAGATT TACAAGAGCT GCAGGCCTCA 
ATGGAACAGT TACTTAGGGA ACAACCTAGT GAAGAATACA GTGAAGAAGA AGAGTCAGTC 
TTAAAGAACA GTGATGTGGA GCCAACTGCA AATGGGACAG ATGTGGCGGA TGAAGATGAC 
AATCCCAGCA GTGAAAGTGC CCTGAACGAA GAATGGCACT CAGATAACAG TGATGGGGAA 
AATGCTAGTG AATGTGAATA CGATAGTGTC TTTAACCATT TAGAGGAACT GAGACTTCAT 
CTGGAGCAGG AAATGGGCTT TGAAAAATTC TTTGAGGTTT ACGAGAAAAT AAAGGCTATT 
CATGAAGATG AAGATGAAAA TATTGAAATT TGTTCAAAAA TAGTTCAAAA TATTTTGGGA 
AATGAACATC AGCATCTTTA TGCCAAGATT CTTCATTTAG TCATGGCAGA TGGAGCCTAC 
CAAGAAGATG ATTGTCCTGC CAGACCTCTA TTGAGCTGGA TAGGGATGGC ACCATGTTCA 
AGAGGCCAAA GAAGAGACCT GGAGCCAGCA AACAGACACA AGGTTTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_028848882.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_028704715.1 Macaca mulatta NIMA related kinase 1 (NEK1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMb86670
Clone ID Related Accession (Same CDS sequence)	XM_028848882.1
Accession Version	XM_028848882.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3867bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-25
Organism	Macaca mulatta(Rhesus monkey)
Product	serine/threonine-protein kinase Nek1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041758.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Macaca mulatta Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG CAGACAGTAT GTTATCAAGG AAATTAACAT CTCAAGAATG 
TCCAGTAAAG AAAGAGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GTTCTCTCTA CATAGTGATG 
GATTACTGTG AGGGAGGGGA TCTGTTTAAG CGAATAAATG CTCAGAAAGG CGTTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTGAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATTAAA TCTCAGAACA TATTTTTAAC TAAAGATGGA 
ACGGTACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AATAATAAAA GTGACATTTG GGCTCTGGGG TGTGTCCTTT ATGAGCTGTG TACACTTAAA 
CATGCTTTTG AAGCTGGCAG TATGAAAAAC TTGGTACTGA AGATAATATC CGGATCTTTT 
CCACCTGTGT CTTTGCATTA TTCCTATGAT CTCCGCAGTT TGGTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC ACCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACGTTT 
TCAAAGTTTG GATCACAGCC TATACCAGCC AAAAGACCAG CTTCAGGACA AAACTCGATT 
TCTGTTATGC CTGCTCAGAA AATTACAAAG CCTGCCGCTA AATATGGAAT ACCTTTAACA 
TATAAGAAAT ATGGAGAGAA AAAATTATAC GAAAAGAAAC CACTGCAAAA ACATAAACAG 
GCCCATCAAA CTCCAGAGAA GAGAGTGAAT ATTGGAGAAG AAAGGAGGAA AATGTCTGAG 
GAAGCAGCAA GAAAGAGAAG GCTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTATTAGTT TAATGAAGGC TGAACAAATG AAAAGGCAAG AAAAGGAAAG GTTGGAAAGA 
ATAAATAGGG CCAGGGAACA AGGATGGAGA AATGTGCTAA GTGCTGGTGG AGGTGGTGAA 
GTAAAGGCTC CTTTTCTTGG CAGTGGAGGG ACTATAGCTC CATCATCTTT TTCTTCTCGA 
GGACAGTATG AACATTACCA TGCCATTTTT GACCAAATGC AACAACAGAG AGCAGAAGAT 
AATGAAGCTA AATGGAAAAG AGAAATATAT GGTCGAGGTC TTCCAGAAAG CGGAATTCTG 
CCTGGAGTTC GTCCAGGATT TCCTTATGGG GCTGCAGGTC ATCACCATTT CCCTGATGCT 
GATGATATTA GAAAAACTTT GAAAAGATTG AAGGCGGTGT CTAAACAAGC CAATGCAAAC 
AGGCAAAAAG GGCAGCTAGC TGTAGAAAGA GCTAAACAAG TAGAAGAGTT CCTGCAGCGA 
AAACGGGAAG CTATGCAGAA TAAAGCTCGA GCCGAAGGAC ATATGGTTTA TCTGGCAAGA 
CTGAGGCAAA TAAGACTACA GAATTTCAAT GAACGCCAAC AGATTAAAGC CAAACTTCGT 
GGTGAAAAGA AAGAAGCTAA TCGTTCTGAA GGACAAGAAG GAAGTGAAGA GGCTGACATG 
AGGCGCAAAA AAATCGAATC CCTGAAGGCC CATGCAAATG CACGTGCTGC TGTACTAAAA 
GAACAACTAG AACGAAAGAG AAAGGAAGCT TATGAGAGAG AAAAAAAAGT GTGGGAAGAG 
CATTTGGTGG CTAAAGGAGT TAAGAGTTCT GATGTTTCTC CACCTTTGGG ACAGCATGAA 
ACAGGTGGCT CTCCATCAAA GCAACAGATG AGATCGGTTA TTTCTGTGAC TTCAGCTTTG 
AAAGAAGTTG GCGTGGACAG TAGTTTAACT GATATCCGGG AAATTTCAGA AGAAATGCAA 
AAGACCAACA ATGCTATTTC AAGTAAGCGA GAAATACTTC GTAGATTAAA TGAAAATCTT 
AAAGCTCAAG AAGATGAAAA AGGAAAGCAG CATCTCTCTG ATACTTGTGA GATAAATGTT 
CTTGAAGATG CCAAAGAGCA TGAAAAAGAA AAATCAGTTT CATCTGATCG CAAGAAGTGG 
GAGGCAGGAG GTCAACTTGT GATTCCTCTG GATGAGTTAA CACTAGATAC ATCCTTCTCT 
ACAACTGAAA GACATACAGT GGGAGAAGTT ATTAAATTAG ATCCTAATGG ATCTCCAAGA 
AGAGTCTGGG GGAAAAGTCC GACAGATTCT GTTCTGAAGA TACTTGGAGA AGCTGAACTA 
CAACTTCAGA CAGAACTATT AGAAAATACA ACTATTAGAA GTGATATTTC TCCTGAAGGG 
GAAAAGTACA AACCCTTAAT TACTGAAGAA AAAAAAGTGC AATGTATTTC ACATGAAATA 
AACCCATCAG CTATTGTTGA TTCTCCTGTT GAGACAAAAA GCCCCGAGTT TACTGAGGCA 
TCTCCACAGA TGTCATTGAA ACTGGAAGGA AATTTAGAAG AACCTGATGA TTTGGAAACA 
GAAGTTCTAC AAGAGCCAAG TGGAACAAAC AAAGATGAGA GCTTGCCATG CACTGTTACT 
GATGTGTGGA TTAGTGAGGA AAAAGAAGCA AAGGAAACTC AGTTGGAAGA TAGGATCACC 
ATTCAAGAAA ATGAAGTTTC TGAGGATGGA GTCTCGAGTA CTGTGGACCA ACTTAGTGAC 
ATTCATATCG AGACGGGAGC CAATGATTCT CAGCACTCTA AATGTGATGT AGATAAGTCT 
GTGCAACCAG AGCCATTTTT CCATAAAGTG GTTCATTCTG AACACTTGAA CTTAGTCCCT 
CAAGTTCAAT CAGTTCAGTG TTCACCAGAA GAATCCTTTG CATTTCGATC TCACTCGCAT 
TTACCACCAA AAAATAAAAA CAAGAATTCC TTGCTGATTG GACTTTCAAC TGGTCTGTTT 
GATGCAAACA ACCCAAAGAT GTTAAGGACA TGTTCACTTC CAGATCTCTC AAAGCTGTTC 
AGAACCCTTA TGGATGTTCC CACCGTAGGA GATGTTCGTC AAGACAATCT TGAAATAGAT 
GAAATTGAAG ATGAAAACAT TAAAGAAGGA CCTTCTGATT CTGAAGACAT TGTGTTTGAA 
GAAACTGACA CAGATTTACA AGAGCTGCAG GCCTCAATGG AACAGTTACT TAGGGAACAA 
CCTAGTGAAG AATACAGTGA AGAAGAAGAG TCAGTCTTAA AGAACAGTGA TGTGGAGCCA 
ACTGCAAATG GGACAGATGT GGCGGATGAA GATGACAATC CCAGCAGTGA AAGTGCCCTG 
AACGAAGAAT GGCACTCAGA TAACAGTGAT GGGGAAAATG CTAGTGAATG TGAATACGAT 
AGTGTCTTTA ACCATTTAGA GGAACTGAGA CTTCATCTGG AGCAGGAAAT GGGCTTTGAA 
AAATTCTTTG AGGTTTACGA GAAAATAAAG GCTATTCATG AAGATGAAGA TGAAAATATT 
GAAATTTGTT CAAAAATAGT TCAAAATATT TTGGGAAATG AACATCAGCA TCTTTATGCC 
AAGATTCTTC ATTTAGTCAT GGCAGATGGA GCCTACCAAG AAGATGATTG TCCTGCCAGA 
CCTCTATTGA GCTGGATAGG GATGGCACCA TGTTCAAGAG GCCAAAGAAG AGACCTGGAG 
CCAGCAAACA GACACAAGGT TTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028704715.1
CDS	632..4498
Translation	MEKYVRLQKIGEGSFGKAILVKSTEDGRQYVIKEINISRMSSKEREESRREVAVLANMKHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGVLFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYELCTLKHAFEAGSMKNLVLKIISGSFPPVSLHYSYDLRSLVSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTFSKFGSQPIPAKRPASGQNSISVMPAQKITKPAAKYGIPLTYKKYGEKKLYEKKPLQKHKQAHQTPEKRVNIGEERRKMSEEAARKRRLEFIEKEKKQKDQIISLMKAEQMKRQEKERLERINRAREQGWRNVLSAGGGGEVKAPFLGSGGTIAPSSFSSRGQYEHYHAIFDQMQQQRAEDNEAKWKREIYGRGLPESGILPGVRPGFPYGAAGHHHFPDADDIRKTLKRLKAVSKQANANRQKGQLAVERAKQVEEFLQRKREAMQNKARAEGHMVYLARLRQIRLQNFNERQQIKAKLRGEKKEANRSEGQEGSEEADMRRKKIESLKAHANARAAVLKEQLERKRKEAYEREKKVWEEHLVAKGVKSSDVSPPLGQHETGGSPSKQQMRSVISVTSALKEVGVDSSLTDIREISEEMQKTNNAISSKREILRRLNENLKAQEDEKGKQHLSDTCEINVLEDAKEHEKEKSVSSDRKKWEAGGQLVIPLDELTLDTSFSTTERHTVGEVIKLDPNGSPRRVWGKSPTDSVLKILGEAELQLQTELLENTTIRSDISPEGEKYKPLITEEKKVQCISHEINPSAIVDSPVETKSPEFTEASPQMSLKLEGNLEEPDDLETEVLQEPSGTNKDESLPCTVTDVWISEEKEAKETQLEDRITIQENEVSEDGVSSTVDQLSDIHIETGANDSQHSKCDVDKSVQPEPFFHKVVHSEHLNLVPQVQSVQCSPEESFAFRSHSHLPPKNKNKNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDVRQDNLEIDEIEDENIKEGPSDSEDIVFEETDTDLQELQASMEQLLREQPSEEYSEEEESVLKNSDVEPTANGTDVADEDDNPSSESALNEEWHSDNSDGENASECEYDSVFNHLEELRLHLEQEMGFEKFFEVYEKIKAIHEDEDENIEICSKIVQNILGNEHQHLYAKILHLVMADGAYQEDDCPARPLLSWIGMAPCSRGQRRDLEPANRHKVY

Target ORF information:

RefSeq Version	XM_028848882.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta NIMA related kinase 1 (NEK1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_028848882.1

ORF Insert Sequence:

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG CAGACAGTAT GTTATCAAGG AAATTAACAT CTCAAGAATG 
TCCAGTAAAG AAAGAGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GTTCTCTCTA CATAGTGATG 
GATTACTGTG AGGGAGGGGA TCTGTTTAAG CGAATAAATG CTCAGAAAGG CGTTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTGAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATTAAA TCTCAGAACA TATTTTTAAC TAAAGATGGA 
ACGGTACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AATAATAAAA GTGACATTTG GGCTCTGGGG TGTGTCCTTT ATGAGCTGTG TACACTTAAA 
CATGCTTTTG AAGCTGGCAG TATGAAAAAC TTGGTACTGA AGATAATATC CGGATCTTTT 
CCACCTGTGT CTTTGCATTA TTCCTATGAT CTCCGCAGTT TGGTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC ACCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACGTTT 
TCAAAGTTTG GATCACAGCC TATACCAGCC AAAAGACCAG CTTCAGGACA AAACTCGATT 
TCTGTTATGC CTGCTCAGAA AATTACAAAG CCTGCCGCTA AATATGGAAT ACCTTTAACA 
TATAAGAAAT ATGGAGAGAA AAAATTATAC GAAAAGAAAC CACTGCAAAA ACATAAACAG 
GCCCATCAAA CTCCAGAGAA GAGAGTGAAT ATTGGAGAAG AAAGGAGGAA AATGTCTGAG 
GAAGCAGCAA GAAAGAGAAG GCTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTATTAGTT TAATGAAGGC TGAACAAATG AAAAGGCAAG AAAAGGAAAG GTTGGAAAGA 
ATAAATAGGG CCAGGGAACA AGGATGGAGA AATGTGCTAA GTGCTGGTGG AGGTGGTGAA 
GTAAAGGCTC CTTTTCTTGG CAGTGGAGGG ACTATAGCTC CATCATCTTT TTCTTCTCGA 
GGACAGTATG AACATTACCA TGCCATTTTT GACCAAATGC AACAACAGAG AGCAGAAGAT 
AATGAAGCTA AATGGAAAAG AGAAATATAT GGTCGAGGTC TTCCAGAAAG CGGAATTCTG 
CCTGGAGTTC GTCCAGGATT TCCTTATGGG GCTGCAGGTC ATCACCATTT CCCTGATGCT 
GATGATATTA GAAAAACTTT GAAAAGATTG AAGGCGGTGT CTAAACAAGC CAATGCAAAC 
AGGCAAAAAG GGCAGCTAGC TGTAGAAAGA GCTAAACAAG TAGAAGAGTT CCTGCAGCGA 
AAACGGGAAG CTATGCAGAA TAAAGCTCGA GCCGAAGGAC ATATGGTTTA TCTGGCAAGA 
CTGAGGCAAA TAAGACTACA GAATTTCAAT GAACGCCAAC AGATTAAAGC CAAACTTCGT 
GGTGAAAAGA AAGAAGCTAA TCGTTCTGAA GGACAAGAAG GAAGTGAAGA GGCTGACATG 
AGGCGCAAAA AAATCGAATC CCTGAAGGCC CATGCAAATG CACGTGCTGC TGTACTAAAA 
GAACAACTAG AACGAAAGAG AAAGGAAGCT TATGAGAGAG AAAAAAAAGT GTGGGAAGAG 
CATTTGGTGG CTAAAGGAGT TAAGAGTTCT GATGTTTCTC CACCTTTGGG ACAGCATGAA 
ACAGGTGGCT CTCCATCAAA GCAACAGATG AGATCGGTTA TTTCTGTGAC TTCAGCTTTG 
AAAGAAGTTG GCGTGGACAG TAGTTTAACT GATATCCGGG AAATTTCAGA AGAAATGCAA 
AAGACCAACA ATGCTATTTC AAGTAAGCGA GAAATACTTC GTAGATTAAA TGAAAATCTT 
AAAGCTCAAG AAGATGAAAA AGGAAAGCAG CATCTCTCTG ATACTTGTGA GATAAATGTT 
CTTGAAGATG CCAAAGAGCA TGAAAAAGAA AAATCAGTTT CATCTGATCG CAAGAAGTGG 
GAGGCAGGAG GTCAACTTGT GATTCCTCTG GATGAGTTAA CACTAGATAC ATCCTTCTCT 
ACAACTGAAA GACATACAGT GGGAGAAGTT ATTAAATTAG ATCCTAATGG ATCTCCAAGA 
AGAGTCTGGG GGAAAAGTCC GACAGATTCT GTTCTGAAGA TACTTGGAGA AGCTGAACTA 
CAACTTCAGA CAGAACTATT AGAAAATACA ACTATTAGAA GTGATATTTC TCCTGAAGGG 
GAAAAGTACA AACCCTTAAT TACTGAAGAA AAAAAAGTGC AATGTATTTC ACATGAAATA 
AACCCATCAG CTATTGTTGA TTCTCCTGTT GAGACAAAAA GCCCCGAGTT TACTGAGGCA 
TCTCCACAGA TGTCATTGAA ACTGGAAGGA AATTTAGAAG AACCTGATGA TTTGGAAACA 
GAAGTTCTAC AAGAGCCAAG TGGAACAAAC AAAGATGAGA GCTTGCCATG CACTGTTACT 
GATGTGTGGA TTAGTGAGGA AAAAGAAGCA AAGGAAACTC AGTTGGAAGA TAGGATCACC 
ATTCAAGAAA ATGAAGTTTC TGAGGATGGA GTCTCGAGTA CTGTGGACCA ACTTAGTGAC 
ATTCATATCG AGACGGGAGC CAATGATTCT CAGCACTCTA AATGTGATGT AGATAAGTCT 
GTGCAACCAG AGCCATTTTT CCATAAAGTG GTTCATTCTG AACACTTGAA CTTAGTCCCT 
CAAGTTCAAT CAGTTCAGTG TTCACCAGAA GAATCCTTTG CATTTCGATC TCACTCGCAT 
TTACCACCAA AAAATAAAAA CAAGAATTCC TTGCTGATTG GACTTTCAAC TGGTCTGTTT 
GATGCAAACA ACCCAAAGAT GTTAAGGACA TGTTCACTTC CAGATCTCTC AAAGCTGTTC 
AGAACCCTTA TGGATGTTCC CACCGTAGGA GATGTTCGTC AAGACAATCT TGAAATAGAT 
GAAATTGAAG ATGAAAACAT TAAAGAAGGA CCTTCTGATT CTGAAGACAT TGTGTTTGAA 
GAAACTGACA CAGATTTACA AGAGCTGCAG GCCTCAATGG AACAGTTACT TAGGGAACAA 
CCTAGTGAAG AATACAGTGA AGAAGAAGAG TCAGTCTTAA AGAACAGTGA TGTGGAGCCA 
ACTGCAAATG GGACAGATGT GGCGGATGAA GATGACAATC CCAGCAGTGA AAGTGCCCTG 
AACGAAGAAT GGCACTCAGA TAACAGTGAT GGGGAAAATG CTAGTGAATG TGAATACGAT 
AGTGTCTTTA ACCATTTAGA GGAACTGAGA CTTCATCTGG AGCAGGAAAT GGGCTTTGAA 
AAATTCTTTG AGGTTTACGA GAAAATAAAG GCTATTCATG AAGATGAAGA TGAAAATATT 
GAAATTTGTT CAAAAATAGT TCAAAATATT TTGGGAAATG AACATCAGCA TCTTTATGCC 
AAGATTCTTC ATTTAGTCAT GGCAGATGGA GCCTACCAAG AAGATGATTG TCCTGCCAGA 
CCTCTATTGA GCTGGATAGG GATGGCACCA TGTTCAAGAG GCCAAAGAAG AGACCTGGAG 
CCAGCAAACA GACACAAGGT TTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_001083275.4 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_001083275.3 Macaca mulatta NIMA related kinase 1 (NEK1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OMb86671
Clone ID Related Accession (Same CDS sequence)	XM_001083275.4
Accession Version	XM_001083275.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3861bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-25
Organism	Macaca mulatta(Rhesus monkey)
Product	serine/threonine-protein kinase Nek1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041758.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 23, 2019 this sequence version replaced XM_001083275.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Macaca mulatta Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG CAGACAGTAT GTTATCAAGG AAATTAACAT CTCAAGAATG 
TCCAGTAAAG AAAGAGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GTTCTCTCTA CATAGTGATG 
GATTACTGTG AGGGAGGGGA TCTGTTTAAG CGAATAAATG CTCAGAAAGG CGTTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTGAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATTAAA TCTCAGAACA TATTTTTAAC TAAAGATGGA 
ACGGTACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AATAATAAAA GTGACATTTG GGCTCTGGGG TGTGTCCTTT ATGAGCTGTG TACACTTAAA 
CATGCTTTTG AAGCTGGCAG TATGAAAAAC TTGGTACTGA AGATAATATC CGGATCTTTT 
CCACCTGTGT CTTTGCATTA TTCCTATGAT CTCCGCAGTT TGGTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC ACCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACGTTT 
TCAAAGTTTG GATCACAGCC TATACCAGCC AAAAGACCAG CTTCAGGACA AAACTCGATT 
TCTGTTATGC CTGCTCAGAA AATTACAAAG CCTGCCGCTA AATATGGAAT ACCTTTAACA 
TATAAGAAAT ATGGAGAGAA AAAATTATAC GAAAAGAAAC CACTGCAAAA ACATAAACAG 
GCCCATCAAA CTCCAGAGAA GAGAGTGAAT ATTGGAGAAG AAAGGAGGAA AATGTCTGAG 
GAAGCAGCAA GAAAGAGAAG GCTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTATTAGTT TAATGAAGGC TGAACAAATG AAAAGGCAAG AAAAGGAAAG GTTGGAAAGA 
ATAAATAGGG CCAGGGAACA AGGATGGAGA AATGTGCTAA GTGCTGGTGG AGGTGGTGAA 
GTAAAGGCTC CTTTTCTTGG CAGTGGAGGG ACTATAGCTC CATCATCTTT TTCTTCTCGA 
GGACAGTATG AACATTACCA TGCCATTTTT GACCAAATGC AACAACAGAG AGCAGAAGAT 
AATGAAGCTA AATGGAAAAG AGAAATATAT GGTCGAGGTC TTCCAGAAAG CGGAATTCTG 
CCTGGAGTTC GTCCAGGATT TCCTTATGGG GCTGCAGGTC ATCACCATTT CCCTGATGCT 
GATGATATTA GAAAAACTTT GAAAAGATTG AAGGCGGTGT CTAAACAAGC CAATGCAAAC 
AGGCAAAAAG GGCAGCTAGC TGTAGAAAGA GCTAAACAAG TAGAAGAGTT CCTGCAGCGA 
AAACGGGAAG CTATGCAGAA TAAAGCTCGA GCCGAAGGAC ATATGGGAAT CCTGCAAAAC 
CTGGCAGCTA TGTATGGAGG CAGGCCCAGC TCTTCAAGAG GAGGGAAGCC AAGGAACAAA 
GAGGAAGAGG TTTATCTGGC AAGACTGAGG CAAATAAGAC TACAGAATTT CAATGAACGC 
CAACAGATTA AAGCCAAACT TCGTGGTGAA AAGAAAGAAG CTAATCGTTC TGAAGGACAA 
GAAGGAAGTG AAGAGGCTGA CATGAGGCGC AAAAAAATCG AATCCCTGAA GGCCCATGCA 
AATGCACGTG CTGCTGTACT AAAAGAACAA CTAGAACGAA AGAGAAAGGA AGCTTATGAG 
AGAGAAAAAA AAGTGTGGGA AGAGCATTTG GTGGCTAAAG GAGTTAAGAG TTCTGATGTT 
TCTCCACCTT TGGGACAGCA TGAAACAGGT GGCTCTCCAT CAAAGCAACA GATGAGATCG 
GTTATTTCTG TGACTTCAGC TTTGAAAGAA GTTGGCGTGG ACAGTAGTTT AACTGATATC 
CGGGAAATTT CAGAAGAAAT GCAAAAGACC AACAATGCTA TTTCAAGTAA GCGAGAAATA 
CTTCGTAGAT TAAATGAAAA TCTTAAAGCT CAAGAAGATG AAAAAGGAAA GCAGCATCTC 
TCTGATACTT GTGAGATAAA TGTTCTTGAA GATGCCAAAG AGCATGAAAA AGAAAAATCA 
GTTTCATCTG ATCGCAAGAA GTGGGAGGCA GGAGGTCAAC TTGTGATTCC TCTGGATGAG 
TTAACACTAG ATACATCCTT CTCTACAACT GAAAGACATA CAGTGGGAGA AGTTATTAAA 
TTAGATCCTA ATGGATCTCC AAGAAGAGTC TGGGGGAAAA GTCCGACAGA TTCTGTTCTG 
AAGATACTTG GAGAAGCTGA ACTACAACTT CAGACAGAAC TATTAGAAAA TACAACTATT 
AGAAGTGATA TTTCTCCTGA AGGGGAAAAG TACAAACCCT TAATTACTGA AGAAAAAAAA 
GTGCAATGTA TTTCACATGA AATAAACCCA TCAGCTATTG TTGATTCTCC TGTTGAGACA 
AAAAGCCCCG AGTTTACTGA GGCATCTCCA CAGATGTCAT TGAAACTGGA AGGAAATTTA 
GAAGAACCTG ATGATTTGGA AACAGAAGTT CTACAAGAGC CAAGTGGAAC AAACAAAGAT 
GAGAGCTTGC CATGCACTGT TACTGATGTG TGGATTAGTG AGGAAAAAGA AGCAAAGGAA 
ACTCAGTTGG AAGATAGGAT CACCATTCAA GAAAATGAAG TTTCTGAGGA TGGAGTCTCG 
AGTACTGTGG ACCAACTTAG TGACATTCAT ATCGAGACGG GAGCCAATGA TTCTCAGCAC 
TCTAAATGTG ATGTAGATAA GTCTGTGCAA CCAGAGCCAT TTTTCCATAA AGTGGTTCAT 
TCTGAACACT TGAACTTAGT CCCTCAAGTT CAATCAGTTC AGTGTTCACC AGAAGAATCC 
TTTGCATTTC GATCTCACTC GCATTTACCA CCAAAAAATA AAAACAAGAA TTCCTTGCTG 
ATTGGACTTT CAACTGGTCT GTTTGATGCA AACAACCCAA AGATGTTAAG GACATGTTCA 
CTTCCAGATC TCTCAAAGCT GTTCAGAACC CTTATGGATG TTCCCACCGT AGGAGATGTT 
CGTCAAGACA ATCTTGAAAT AGATGAAATT GAAGATGAAA ACATTAAAGA AGGACCTTCT 
GATTCTGAAG ACATTGTGTT TGAAGAAACT GACACAGATT TACAAGAGCT GCAGGCCTCA 
ATGGAACAGT TACTTAGGGA ACAACCTAGT GAAGAATACA GTGAAGAAGA AGAGTCAGTC 
TTAAAGAACA GTGATGTGGA GCCAACTGCA AATGGGACAG ATGTGGCGGA TGAAGATGAC 
AATCCCAGCA GTGAAAGTGC CCTGAACGAA GAATGGCACT CAGATAACAG TGATGGGGAA 
AATGCTAGTG AATGTGAATA CGATAGTGTC TTTAACCATT TAGAGGAACT GAGACTTCAT 
CTGGAGCAGG AAATGGGCTT TGAAAAATTC TTTGAGGTTT ACGAGAAAAT AAAGGCTATT 
CATGAAGATG AAGATGAAAA TATTGAAATT TGTTCAAAAA TAGTTCAAAA TATTTTGGGA 
AATGAACATC AGCATCTTTA TGCCAAGATT CTTCATTTAG TCATGGCAGA TGGAGCCTAC 
CAAGAAGATA ATGATGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001083275.3
CDS	575..4435
Translation	MEKYVRLQKIGEGSFGKAILVKSTEDGRQYVIKEINISRMSSKEREESRREVAVLANMKHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGVLFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYELCTLKHAFEAGSMKNLVLKIISGSFPPVSLHYSYDLRSLVSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTFSKFGSQPIPAKRPASGQNSISVMPAQKITKPAAKYGIPLTYKKYGEKKLYEKKPLQKHKQAHQTPEKRVNIGEERRKMSEEAARKRRLEFIEKEKKQKDQIISLMKAEQMKRQEKERLERINRAREQGWRNVLSAGGGGEVKAPFLGSGGTIAPSSFSSRGQYEHYHAIFDQMQQQRAEDNEAKWKREIYGRGLPESGILPGVRPGFPYGAAGHHHFPDADDIRKTLKRLKAVSKQANANRQKGQLAVERAKQVEEFLQRKREAMQNKARAEGHMGILQNLAAMYGGRPSSSRGGKPRNKEEEVYLARLRQIRLQNFNERQQIKAKLRGEKKEANRSEGQEGSEEADMRRKKIESLKAHANARAAVLKEQLERKRKEAYEREKKVWEEHLVAKGVKSSDVSPPLGQHETGGSPSKQQMRSVISVTSALKEVGVDSSLTDIREISEEMQKTNNAISSKREILRRLNENLKAQEDEKGKQHLSDTCEINVLEDAKEHEKEKSVSSDRKKWEAGGQLVIPLDELTLDTSFSTTERHTVGEVIKLDPNGSPRRVWGKSPTDSVLKILGEAELQLQTELLENTTIRSDISPEGEKYKPLITEEKKVQCISHEINPSAIVDSPVETKSPEFTEASPQMSLKLEGNLEEPDDLETEVLQEPSGTNKDESLPCTVTDVWISEEKEAKETQLEDRITIQENEVSEDGVSSTVDQLSDIHIETGANDSQHSKCDVDKSVQPEPFFHKVVHSEHLNLVPQVQSVQCSPEESFAFRSHSHLPPKNKNKNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDVRQDNLEIDEIEDENIKEGPSDSEDIVFEETDTDLQELQASMEQLLREQPSEEYSEEEESVLKNSDVEPTANGTDVADEDDNPSSESALNEEWHSDNSDGENASECEYDSVFNHLEELRLHLEQEMGFEKFFEVYEKIKAIHEDEDENIEICSKIVQNILGNEHQHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_001083275.4
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta NIMA related kinase 1 (NEK1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001083275.4

ORF Insert Sequence:

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG CAGACAGTAT GTTATCAAGG AAATTAACAT CTCAAGAATG 
TCCAGTAAAG AAAGAGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GTTCTCTCTA CATAGTGATG 
GATTACTGTG AGGGAGGGGA TCTGTTTAAG CGAATAAATG CTCAGAAAGG CGTTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTGAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATTAAA TCTCAGAACA TATTTTTAAC TAAAGATGGA 
ACGGTACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AATAATAAAA GTGACATTTG GGCTCTGGGG TGTGTCCTTT ATGAGCTGTG TACACTTAAA 
CATGCTTTTG AAGCTGGCAG TATGAAAAAC TTGGTACTGA AGATAATATC CGGATCTTTT 
CCACCTGTGT CTTTGCATTA TTCCTATGAT CTCCGCAGTT TGGTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC ACCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACGTTT 
TCAAAGTTTG GATCACAGCC TATACCAGCC AAAAGACCAG CTTCAGGACA AAACTCGATT 
TCTGTTATGC CTGCTCAGAA AATTACAAAG CCTGCCGCTA AATATGGAAT ACCTTTAACA 
TATAAGAAAT ATGGAGAGAA AAAATTATAC GAAAAGAAAC CACTGCAAAA ACATAAACAG 
GCCCATCAAA CTCCAGAGAA GAGAGTGAAT ATTGGAGAAG AAAGGAGGAA AATGTCTGAG 
GAAGCAGCAA GAAAGAGAAG GCTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTATTAGTT TAATGAAGGC TGAACAAATG AAAAGGCAAG AAAAGGAAAG GTTGGAAAGA 
ATAAATAGGG CCAGGGAACA AGGATGGAGA AATGTGCTAA GTGCTGGTGG AGGTGGTGAA 
GTAAAGGCTC CTTTTCTTGG CAGTGGAGGG ACTATAGCTC CATCATCTTT TTCTTCTCGA 
GGACAGTATG AACATTACCA TGCCATTTTT GACCAAATGC AACAACAGAG AGCAGAAGAT 
AATGAAGCTA AATGGAAAAG AGAAATATAT GGTCGAGGTC TTCCAGAAAG CGGAATTCTG 
CCTGGAGTTC GTCCAGGATT TCCTTATGGG GCTGCAGGTC ATCACCATTT CCCTGATGCT 
GATGATATTA GAAAAACTTT GAAAAGATTG AAGGCGGTGT CTAAACAAGC CAATGCAAAC 
AGGCAAAAAG GGCAGCTAGC TGTAGAAAGA GCTAAACAAG TAGAAGAGTT CCTGCAGCGA 
AAACGGGAAG CTATGCAGAA TAAAGCTCGA GCCGAAGGAC ATATGGGAAT CCTGCAAAAC 
CTGGCAGCTA TGTATGGAGG CAGGCCCAGC TCTTCAAGAG GAGGGAAGCC AAGGAACAAA 
GAGGAAGAGG TTTATCTGGC AAGACTGAGG CAAATAAGAC TACAGAATTT CAATGAACGC 
CAACAGATTA AAGCCAAACT TCGTGGTGAA AAGAAAGAAG CTAATCGTTC TGAAGGACAA 
GAAGGAAGTG AAGAGGCTGA CATGAGGCGC AAAAAAATCG AATCCCTGAA GGCCCATGCA 
AATGCACGTG CTGCTGTACT AAAAGAACAA CTAGAACGAA AGAGAAAGGA AGCTTATGAG 
AGAGAAAAAA AAGTGTGGGA AGAGCATTTG GTGGCTAAAG GAGTTAAGAG TTCTGATGTT 
TCTCCACCTT TGGGACAGCA TGAAACAGGT GGCTCTCCAT CAAAGCAACA GATGAGATCG 
GTTATTTCTG TGACTTCAGC TTTGAAAGAA GTTGGCGTGG ACAGTAGTTT AACTGATATC 
CGGGAAATTT CAGAAGAAAT GCAAAAGACC AACAATGCTA TTTCAAGTAA GCGAGAAATA 
CTTCGTAGAT TAAATGAAAA TCTTAAAGCT CAAGAAGATG AAAAAGGAAA GCAGCATCTC 
TCTGATACTT GTGAGATAAA TGTTCTTGAA GATGCCAAAG AGCATGAAAA AGAAAAATCA 
GTTTCATCTG ATCGCAAGAA GTGGGAGGCA GGAGGTCAAC TTGTGATTCC TCTGGATGAG 
TTAACACTAG ATACATCCTT CTCTACAACT GAAAGACATA CAGTGGGAGA AGTTATTAAA 
TTAGATCCTA ATGGATCTCC AAGAAGAGTC TGGGGGAAAA GTCCGACAGA TTCTGTTCTG 
AAGATACTTG GAGAAGCTGA ACTACAACTT CAGACAGAAC TATTAGAAAA TACAACTATT 
AGAAGTGATA TTTCTCCTGA AGGGGAAAAG TACAAACCCT TAATTACTGA AGAAAAAAAA 
GTGCAATGTA TTTCACATGA AATAAACCCA TCAGCTATTG TTGATTCTCC TGTTGAGACA 
AAAAGCCCCG AGTTTACTGA GGCATCTCCA CAGATGTCAT TGAAACTGGA AGGAAATTTA 
GAAGAACCTG ATGATTTGGA AACAGAAGTT CTACAAGAGC CAAGTGGAAC AAACAAAGAT 
GAGAGCTTGC CATGCACTGT TACTGATGTG TGGATTAGTG AGGAAAAAGA AGCAAAGGAA 
ACTCAGTTGG AAGATAGGAT CACCATTCAA GAAAATGAAG TTTCTGAGGA TGGAGTCTCG 
AGTACTGTGG ACCAACTTAG TGACATTCAT ATCGAGACGG GAGCCAATGA TTCTCAGCAC 
TCTAAATGTG ATGTAGATAA GTCTGTGCAA CCAGAGCCAT TTTTCCATAA AGTGGTTCAT 
TCTGAACACT TGAACTTAGT CCCTCAAGTT CAATCAGTTC AGTGTTCACC AGAAGAATCC 
TTTGCATTTC GATCTCACTC GCATTTACCA CCAAAAAATA AAAACAAGAA TTCCTTGCTG 
ATTGGACTTT CAACTGGTCT GTTTGATGCA AACAACCCAA AGATGTTAAG GACATGTTCA 
CTTCCAGATC TCTCAAAGCT GTTCAGAACC CTTATGGATG TTCCCACCGT AGGAGATGTT 
CGTCAAGACA ATCTTGAAAT AGATGAAATT GAAGATGAAA ACATTAAAGA AGGACCTTCT 
GATTCTGAAG ACATTGTGTT TGAAGAAACT GACACAGATT TACAAGAGCT GCAGGCCTCA 
ATGGAACAGT TACTTAGGGA ACAACCTAGT GAAGAATACA GTGAAGAAGA AGAGTCAGTC 
TTAAAGAACA GTGATGTGGA GCCAACTGCA AATGGGACAG ATGTGGCGGA TGAAGATGAC 
AATCCCAGCA GTGAAAGTGC CCTGAACGAA GAATGGCACT CAGATAACAG TGATGGGGAA 
AATGCTAGTG AATGTGAATA CGATAGTGTC TTTAACCATT TAGAGGAACT GAGACTTCAT 
CTGGAGCAGG AAATGGGCTT TGAAAAATTC TTTGAGGTTT ACGAGAAAAT AAAGGCTATT 
CATGAAGATG AAGATGAAAA TATTGAAATT TGTTCAAAAA TAGTTCAAAA TATTTTGGGA 
AATGAACATC AGCATCTTTA TGCCAAGATT CTTCATTTAG TCATGGCAGA TGGAGCCTAC 
CAAGAAGATA ATGATGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_028848883.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_028704716.1 Macaca mulatta NIMA related kinase 1 (NEK1), transcript variant X6, mRNA.

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CloneID	OMb86672
Clone ID Related Accession (Same CDS sequence)	XM_028848883.1
Accession Version	XM_028848883.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3819bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-25
Organism	Macaca mulatta(Rhesus monkey)
Product	serine/threonine-protein kinase Nek1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041758.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Macaca mulatta Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG CAGACAGTAT GTTATCAAGG AAATTAACAT CTCAAGAATG 
TCCAGTAAAG AAAGAGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GTTCTCTCTA CATAGTGATG 
GATTACTGTG AGGGAGGGGA TCTGTTTAAG CGAATAAATG CTCAGAAAGG CGTTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTGAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATTAAA TCTCAGAACA TATTTTTAAC TAAAGATGGA 
ACGGTACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AATAATAAAA GTGACATTTG GGCTCTGGGG TGTGTCCTTT ATGAGCTGTG TACACTTAAA 
CATGCTTTTG AAGCTGGCAG TATGAAAAAC TTGGTACTGA AGATAATATC CGGATCTTTT 
CCACCTGTGT CTTTGCATTA TTCCTATGAT CTCCGCAGTT TGGTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC ACCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACGTTT 
TCAAAGTTTG GATCACAGCC TATACCAGCC AAAAGACCAG CTTCAGGACA AAACTCGATT 
TCTGTTATGC CTGCTCAGAA AATTACAAAG CCTGCCGCTA AATATGGAAT ACCTTTAACA 
TATAAGAAAT ATGGAGAGAA AAAATTATAC GAAAAGAAAC CACTGCAAAA ACATAAACAG 
GCCCATCAAA CTCCAGAGAA GAGAGTGAAT ATTGGAGAAG AAAGGAGGAA AATGTCTGAG 
GAAGCAGCAA GAAAGAGAAG GCTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTATTAGTT TAATGAAGGC TGAACAAATG AAAAGGCAAG AAAAGGAAAG GTTGGAAAGA 
ATAAATAGGG CCAGGGAACA AGGATGGAGA AATGTGCTAA GTGCTGGTGG AGGTGGTGAA 
GTAAAGGCTC CTTTTCTTGG CAGTGGAGGG ACTATAGCTC CATCATCTTT TTCTTCTCGA 
GGACAGTATG AACATTACCA TGCCATTTTT GACCAAATGC AACAACAGAG AGCAGAAGAT 
AATGAAGCTA AATGGAAAAG AGAAATATAT GGTCGAGGTC TTCCAGAAAG GCAAAAAGGG 
CAGCTAGCTG TAGAAAGAGC TAAACAAGTA GAAGAGTTCC TGCAGCGAAA ACGGGAAGCT 
ATGCAGAATA AAGCTCGAGC CGAAGGACAT ATGGGAATCC TGCAAAACCT GGCAGCTATG 
TATGGAGGCA GGCCCAGCTC TTCAAGAGGA GGGAAGCCAA GGAACAAAGA GGAAGAGGTT 
TATCTGGCAA GACTGAGGCA AATAAGACTA CAGAATTTCA ATGAACGCCA ACAGATTAAA 
GCCAAACTTC GTGGTGAAAA GAAAGAAGCT AATCGTTCTG AAGGACAAGA AGGAAGTGAA 
GAGGCTGACA TGAGGCGCAA AAAAATCGAA TCCCTGAAGG CCCATGCAAA TGCACGTGCT 
GCTGTACTAA AAGAACAACT AGAACGAAAG AGAAAGGAAG CTTATGAGAG AGAAAAAAAA 
GTGTGGGAAG AGCATTTGGT GGCTAAAGGA GTTAAGAGTT CTGATGTTTC TCCACCTTTG 
GGACAGCATG AAACAGGTGG CTCTCCATCA AAGCAACAGA TGAGATCGGT TATTTCTGTG 
ACTTCAGCTT TGAAAGAAGT TGGCGTGGAC AGTAGTTTAA CTGATATCCG GGAAATTTCA 
GAAGAAATGC AAAAGACCAA CAATGCTATT TCAAGTAAGC GAGAAATACT TCGTAGATTA 
AATGAAAATC TTAAAGCTCA AGAAGATGAA AAAGGAAAGC AGCATCTCTC TGATACTTGT 
GAGATAAATG TTCTTGAAGA TGCCAAAGAG CATGAAAAAG AAAAATCAGT TTCATCTGAT 
CGCAAGAAGT GGGAGGCAGG AGGTCAACTT GTGATTCCTC TGGATGAGTT AACACTAGAT 
ACATCCTTCT CTACAACTGA AAGACATACA GTGGGAGAAG TTATTAAATT AGATCCTAAT 
GGATCTCCAA GAAGAGTCTG GGGGAAAAGT CCGACAGATT CTGTTCTGAA GATACTTGGA 
GAAGCTGAAC TACAACTTCA GACAGAACTA TTAGAAAATA CAACTATTAG AAGTGATATT 
TCTCCTGAAG GGGAAAAGTA CAAACCCTTA ATTACTGAAG AAAAAAAAGT GCAATGTATT 
TCACATGAAA TAAACCCATC AGCTATTGTT GATTCTCCTG TTGAGACAAA AAGCCCCGAG 
TTTACTGAGG CATCTCCACA GATGTCATTG AAACTGGAAG GAAATTTAGA AGAACCTGAT 
GATTTGGAAA CAGAAGTTCT ACAAGAGCCA AGTGGAACAA ACAAAGATGA GAGCTTGCCA 
TGCACTGTTA CTGATGTGTG GATTAGTGAG GAAAAAGAAG CAAAGGAAAC TCAGTTGGAA 
GATAGGATCA CCATTCAAGA AAATGAAGTT TCTGAGGATG GAGTCTCGAG TACTGTGGAC 
CAACTTAGTG ACATTCATAT CGAGACGGGA GCCAATGATT CTCAGCACTC TAAATGTGAT 
GTAGATAAGT CTGTGCAACC AGAGCCATTT TTCCATAAAG TGGTTCATTC TGAACACTTG 
AACTTAGTCC CTCAAGTTCA ATCAGTTCAG TGTTCACCAG AAGAATCCTT TGCATTTCGA 
TCTCACTCGC ATTTACCACC AAAAAATAAA AACAAGAATT CCTTGCTGAT TGGACTTTCA 
ACTGGTCTGT TTGATGCAAA CAACCCAAAG ATGTTAAGGA CATGTTCACT TCCAGATCTC 
TCAAAGCTGT TCAGAACCCT TATGGATGTT CCCACCGTAG GAGATGTTCG TCAAGACAAT 
CTTGAAATAG ATGAAATTGA AGATGAAAAC ATTAAAGAAG GACCTTCTGA TTCTGAAGAC 
ATTGTGTTTG AAGAAACTGA CACAGATTTA CAAGAGCTGC AGGCCTCAAT GGAACAGTTA 
CTTAGGGAAC AACCTAGTGA AGAATACAGT GAAGAAGAAG AGTCAGTCTT AAAGAACAGT 
GATGTGGAGC CAACTGCAAA TGGGACAGAT GTGGCGGATG AAGATGACAA TCCCAGCAGT 
GAAAGTGCCC TGAACGAAGA ATGGCACTCA GATAACAGTG ATGGGGAAAA TGCTAGTGAA 
TGTGAATACG ATAGTGTCTT TAACCATTTA GAGGAACTGA GACTTCATCT GGAGCAGGAA 
ATGGGCTTTG AAAAATTCTT TGAGGTTTAC GAGAAAATAA AGGCTATTCA TGAAGATGAA 
GATGAAAATA TTGAAATTTG TTCAAAAATA GTTCAAAATA TTTTGGGAAA TGAACATCAG 
CATCTTTATG CCAAGATTCT TCATTTAGTC ATGGCAGATG GAGCCTACCA AGAAGATGAT 
TGTCCTGCCA GACCTCTATT GAGCTGGATA GGGATGGCAC CATGTTCAAG AGGCCAAAGA 
AGAGACCTGG AGCCAGCAAA CAGACACAAG GTTTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028704716.1
CDS	633..4451
Translation	MEKYVRLQKIGEGSFGKAILVKSTEDGRQYVIKEINISRMSSKEREESRREVAVLANMKHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGVLFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYELCTLKHAFEAGSMKNLVLKIISGSFPPVSLHYSYDLRSLVSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTFSKFGSQPIPAKRPASGQNSISVMPAQKITKPAAKYGIPLTYKKYGEKKLYEKKPLQKHKQAHQTPEKRVNIGEERRKMSEEAARKRRLEFIEKEKKQKDQIISLMKAEQMKRQEKERLERINRAREQGWRNVLSAGGGGEVKAPFLGSGGTIAPSSFSSRGQYEHYHAIFDQMQQQRAEDNEAKWKREIYGRGLPERQKGQLAVERAKQVEEFLQRKREAMQNKARAEGHMGILQNLAAMYGGRPSSSRGGKPRNKEEEVYLARLRQIRLQNFNERQQIKAKLRGEKKEANRSEGQEGSEEADMRRKKIESLKAHANARAAVLKEQLERKRKEAYEREKKVWEEHLVAKGVKSSDVSPPLGQHETGGSPSKQQMRSVISVTSALKEVGVDSSLTDIREISEEMQKTNNAISSKREILRRLNENLKAQEDEKGKQHLSDTCEINVLEDAKEHEKEKSVSSDRKKWEAGGQLVIPLDELTLDTSFSTTERHTVGEVIKLDPNGSPRRVWGKSPTDSVLKILGEAELQLQTELLENTTIRSDISPEGEKYKPLITEEKKVQCISHEINPSAIVDSPVETKSPEFTEASPQMSLKLEGNLEEPDDLETEVLQEPSGTNKDESLPCTVTDVWISEEKEAKETQLEDRITIQENEVSEDGVSSTVDQLSDIHIETGANDSQHSKCDVDKSVQPEPFFHKVVHSEHLNLVPQVQSVQCSPEESFAFRSHSHLPPKNKNKNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDVRQDNLEIDEIEDENIKEGPSDSEDIVFEETDTDLQELQASMEQLLREQPSEEYSEEEESVLKNSDVEPTANGTDVADEDDNPSSESALNEEWHSDNSDGENASECEYDSVFNHLEELRLHLEQEMGFEKFFEVYEKIKAIHEDEDENIEICSKIVQNILGNEHQHLYAKILHLVMADGAYQEDDCPARPLLSWIGMAPCSRGQRRDLEPANRHKVY

Target ORF information:

RefSeq Version	XM_028848883.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta NIMA related kinase 1 (NEK1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_028848883.1

ORF Insert Sequence:

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG CAGACAGTAT GTTATCAAGG AAATTAACAT CTCAAGAATG 
TCCAGTAAAG AAAGAGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GTTCTCTCTA CATAGTGATG 
GATTACTGTG AGGGAGGGGA TCTGTTTAAG CGAATAAATG CTCAGAAAGG CGTTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTGAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATTAAA TCTCAGAACA TATTTTTAAC TAAAGATGGA 
ACGGTACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AATAATAAAA GTGACATTTG GGCTCTGGGG TGTGTCCTTT ATGAGCTGTG TACACTTAAA 
CATGCTTTTG AAGCTGGCAG TATGAAAAAC TTGGTACTGA AGATAATATC CGGATCTTTT 
CCACCTGTGT CTTTGCATTA TTCCTATGAT CTCCGCAGTT TGGTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC ACCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACGTTT 
TCAAAGTTTG GATCACAGCC TATACCAGCC AAAAGACCAG CTTCAGGACA AAACTCGATT 
TCTGTTATGC CTGCTCAGAA AATTACAAAG CCTGCCGCTA AATATGGAAT ACCTTTAACA 
TATAAGAAAT ATGGAGAGAA AAAATTATAC GAAAAGAAAC CACTGCAAAA ACATAAACAG 
GCCCATCAAA CTCCAGAGAA GAGAGTGAAT ATTGGAGAAG AAAGGAGGAA AATGTCTGAG 
GAAGCAGCAA GAAAGAGAAG GCTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTATTAGTT TAATGAAGGC TGAACAAATG AAAAGGCAAG AAAAGGAAAG GTTGGAAAGA 
ATAAATAGGG CCAGGGAACA AGGATGGAGA AATGTGCTAA GTGCTGGTGG AGGTGGTGAA 
GTAAAGGCTC CTTTTCTTGG CAGTGGAGGG ACTATAGCTC CATCATCTTT TTCTTCTCGA 
GGACAGTATG AACATTACCA TGCCATTTTT GACCAAATGC AACAACAGAG AGCAGAAGAT 
AATGAAGCTA AATGGAAAAG AGAAATATAT GGTCGAGGTC TTCCAGAAAG GCAAAAAGGG 
CAGCTAGCTG TAGAAAGAGC TAAACAAGTA GAAGAGTTCC TGCAGCGAAA ACGGGAAGCT 
ATGCAGAATA AAGCTCGAGC CGAAGGACAT ATGGGAATCC TGCAAAACCT GGCAGCTATG 
TATGGAGGCA GGCCCAGCTC TTCAAGAGGA GGGAAGCCAA GGAACAAAGA GGAAGAGGTT 
TATCTGGCAA GACTGAGGCA AATAAGACTA CAGAATTTCA ATGAACGCCA ACAGATTAAA 
GCCAAACTTC GTGGTGAAAA GAAAGAAGCT AATCGTTCTG AAGGACAAGA AGGAAGTGAA 
GAGGCTGACA TGAGGCGCAA AAAAATCGAA TCCCTGAAGG CCCATGCAAA TGCACGTGCT 
GCTGTACTAA AAGAACAACT AGAACGAAAG AGAAAGGAAG CTTATGAGAG AGAAAAAAAA 
GTGTGGGAAG AGCATTTGGT GGCTAAAGGA GTTAAGAGTT CTGATGTTTC TCCACCTTTG 
GGACAGCATG AAACAGGTGG CTCTCCATCA AAGCAACAGA TGAGATCGGT TATTTCTGTG 
ACTTCAGCTT TGAAAGAAGT TGGCGTGGAC AGTAGTTTAA CTGATATCCG GGAAATTTCA 
GAAGAAATGC AAAAGACCAA CAATGCTATT TCAAGTAAGC GAGAAATACT TCGTAGATTA 
AATGAAAATC TTAAAGCTCA AGAAGATGAA AAAGGAAAGC AGCATCTCTC TGATACTTGT 
GAGATAAATG TTCTTGAAGA TGCCAAAGAG CATGAAAAAG AAAAATCAGT TTCATCTGAT 
CGCAAGAAGT GGGAGGCAGG AGGTCAACTT GTGATTCCTC TGGATGAGTT AACACTAGAT 
ACATCCTTCT CTACAACTGA AAGACATACA GTGGGAGAAG TTATTAAATT AGATCCTAAT 
GGATCTCCAA GAAGAGTCTG GGGGAAAAGT CCGACAGATT CTGTTCTGAA GATACTTGGA 
GAAGCTGAAC TACAACTTCA GACAGAACTA TTAGAAAATA CAACTATTAG AAGTGATATT 
TCTCCTGAAG GGGAAAAGTA CAAACCCTTA ATTACTGAAG AAAAAAAAGT GCAATGTATT 
TCACATGAAA TAAACCCATC AGCTATTGTT GATTCTCCTG TTGAGACAAA AAGCCCCGAG 
TTTACTGAGG CATCTCCACA GATGTCATTG AAACTGGAAG GAAATTTAGA AGAACCTGAT 
GATTTGGAAA CAGAAGTTCT ACAAGAGCCA AGTGGAACAA ACAAAGATGA GAGCTTGCCA 
TGCACTGTTA CTGATGTGTG GATTAGTGAG GAAAAAGAAG CAAAGGAAAC TCAGTTGGAA 
GATAGGATCA CCATTCAAGA AAATGAAGTT TCTGAGGATG GAGTCTCGAG TACTGTGGAC 
CAACTTAGTG ACATTCATAT CGAGACGGGA GCCAATGATT CTCAGCACTC TAAATGTGAT 
GTAGATAAGT CTGTGCAACC AGAGCCATTT TTCCATAAAG TGGTTCATTC TGAACACTTG 
AACTTAGTCC CTCAAGTTCA ATCAGTTCAG TGTTCACCAG AAGAATCCTT TGCATTTCGA 
TCTCACTCGC ATTTACCACC AAAAAATAAA AACAAGAATT CCTTGCTGAT TGGACTTTCA 
ACTGGTCTGT TTGATGCAAA CAACCCAAAG ATGTTAAGGA CATGTTCACT TCCAGATCTC 
TCAAAGCTGT TCAGAACCCT TATGGATGTT CCCACCGTAG GAGATGTTCG TCAAGACAAT 
CTTGAAATAG ATGAAATTGA AGATGAAAAC ATTAAAGAAG GACCTTCTGA TTCTGAAGAC 
ATTGTGTTTG AAGAAACTGA CACAGATTTA CAAGAGCTGC AGGCCTCAAT GGAACAGTTA 
CTTAGGGAAC AACCTAGTGA AGAATACAGT GAAGAAGAAG AGTCAGTCTT AAAGAACAGT 
GATGTGGAGC CAACTGCAAA TGGGACAGAT GTGGCGGATG AAGATGACAA TCCCAGCAGT 
GAAAGTGCCC TGAACGAAGA ATGGCACTCA GATAACAGTG ATGGGGAAAA TGCTAGTGAA 
TGTGAATACG ATAGTGTCTT TAACCATTTA GAGGAACTGA GACTTCATCT GGAGCAGGAA 
ATGGGCTTTG AAAAATTCTT TGAGGTTTAC GAGAAAATAA AGGCTATTCA TGAAGATGAA 
GATGAAAATA TTGAAATTTG TTCAAAAATA GTTCAAAATA TTTTGGGAAA TGAACATCAG 
CATCTTTATG CCAAGATTCT TCATTTAGTC ATGGCAGATG GAGCCTACCA AGAAGATGAT 
TGTCCTGCCA GACCTCTATT GAGCTGGATA GGGATGGCAC CATGTTCAAG AGGCCAAAGA 
AGAGACCTGG AGCCAGCAAA CAGACACAAG GTTTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_015139492.2 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_014994978.2 Macaca mulatta NIMA related kinase 1 (NEK1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OMb86673
Clone ID Related Accession (Same CDS sequence)	XM_015139492.2
Accession Version	XM_015139492.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3777bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-25
Organism	Macaca mulatta(Rhesus monkey)
Product	serine/threonine-protein kinase Nek1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041758.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 23, 2019 this sequence version replaced XM_015139492.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Macaca mulatta Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG CAGACAGTAT GTTATCAAGG AAATTAACAT CTCAAGAATG 
TCCAGTAAAG AAAGAGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GTTCTCTCTA CATAGTGATG 
GATTACTGTG AGGGAGGGGA TCTGTTTAAG CGAATAAATG CTCAGAAAGG CGTTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTGAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATTAAA TCTCAGAACA TATTTTTAAC TAAAGATGGA 
ACGGTACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AATAATAAAA GTGACATTTG GGCTCTGGGG TGTGTCCTTT ATGAGCTGTG TACACTTAAA 
CATGCTTTTG AAGCTGGCAG TATGAAAAAC TTGGTACTGA AGATAATATC CGGATCTTTT 
CCACCTGTGT CTTTGCATTA TTCCTATGAT CTCCGCAGTT TGGTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC ACCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACGTTT 
TCAAAGTTTG GATCACAGCC TATACCAGCC AAAAGACCAG CTTCAGGACA AAACTCGATT 
TCTGTTATGC CTGCTCAGAA AATTACAAAG CCTGCCGCTA AATATGGAAT ACCTTTAACA 
TATAAGAAAT ATGGAGAGAA AAAATTATAC GAAAAGAAAC CACTGCAAAA ACATAAACAG 
GCCCATCAAA CTCCAGAGAA GAGAGTGAAT ATTGGAGAAG AAAGGAGGAA AATGTCTGAG 
GAAGCAGCAA GAAAGAGAAG GCTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTATTAGTT TAATGAAGGC TGAACAAATG AAAAGGCAAG AAAAGGAAAG GTTGGAAAGA 
ATAAATAGGG CCAGGGAACA AGGATGGAGA AATGTGCTAA GTGCTGGTGG AGGTGGTGAA 
GTAAAGGCTC CTTTTCTTGG CAGTGGAGGG ACTATAGCTC CATCATCTTT TTCTTCTCGA 
GGACAGTATG AACATTACCA TGCCATTTTT GACCAAATGC AACAACAGAG AGCAGAAGAT 
AATGAAGCTA AATGGAAAAG AGAAATATAT GGTCGAGGTC TTCCAGAAAG CGGAATTCTG 
CCTGGAGTTC GTCCAGGATT TCCTTATGGG GCTGCAGGTC ATCACCATTT CCCTGATGCT 
GATGATATTA GAAAAACTTT GAAAAGATTG AAGGCGGTGT CTAAACAAGC CAATGCAAAC 
AGGCAAAAAG GGCAGCTAGC TGTAGAAAGA GCTAAACAAG TAGAAGAGTT CCTGCAGCGA 
AAACGGGAAG CTATGCAGAA TAAAGCTCGA GCCGAAGGAC ATATGGTTTA TCTGGCAAGA 
CTGAGGCAAA TAAGACTACA GAATTTCAAT GAACGCCAAC AGATTAAAGC CAAACTTCGT 
GGTGAAAAGA AAGAAGCTAA TCGTTCTGAA GGACAAGAAG GAAGTGAAGA GGCTGACATG 
AGGCGCAAAA AAATCGAATC CCTGAAGGCC CATGCAAATG CACGTGCTGC TGTACTAAAA 
GAACAACTAG AACGAAAGAG AAAGGAAGCT TATGAGAGAG AAAAAAAAGT GTGGGAAGAG 
CATTTGGTGG CTAAAGGAGT TAAGAGTTCT GATGTTTCTC CACCTTTGGG ACAGCATGAA 
ACAGGTGGCT CTCCATCAAA GCAACAGATG AGATCGGTTA TTTCTGTGAC TTCAGCTTTG 
AAAGAAGTTG GCGTGGACAG TAGTTTAACT GATATCCGGG AAATTTCAGA AGAAATGCAA 
AAGACCAACA ATGCTATTTC AAGTAAGCGA GAAATACTTC GTAGATTAAA TGAAAATCTT 
AAAGCTCAAG AAGATGAAAA AGGAAAGCAG CATCTCTCTG ATACTTGTGA GATAAATGTT 
CTTGAAGATG CCAAAGAGCA TGAAAAAGAA AAATCAGTTT CATCTGATCG CAAGAAGTGG 
GAGGCAGGAG GTCAACTTGT GATTCCTCTG GATGAGTTAA CACTAGATAC ATCCTTCTCT 
ACAACTGAAA GACATACAGT GGGAGAAGTT ATTAAATTAG ATCCTAATGG ATCTCCAAGA 
AGAGTCTGGG GGAAAAGTCC GACAGATTCT GTTCTGAAGA TACTTGGAGA AGCTGAACTA 
CAACTTCAGA CAGAACTATT AGAAAATACA ACTATTAGAA GTGATATTTC TCCTGAAGGG 
GAAAAGTACA AACCCTTAAT TACTGAAGAA AAAAAAGTGC AATGTATTTC ACATGAAATA 
AACCCATCAG CTATTGTTGA TTCTCCTGTT GAGACAAAAA GCCCCGAGTT TACTGAGGCA 
TCTCCACAGA TGTCATTGAA ACTGGAAGGA AATTTAGAAG AACCTGATGA TTTGGAAACA 
GAAGTTCTAC AAGAGCCAAG TGGAACAAAC AAAGATGAGA GCTTGCCATG CACTGTTACT 
GATGTGTGGA TTAGTGAGGA AAAAGAAGCA AAGGAAACTC AGTTGGAAGA TAGGATCACC 
ATTCAAGAAA ATGAAGTTTC TGAGGATGGA GTCTCGAGTA CTGTGGACCA ACTTAGTGAC 
ATTCATATCG AGACGGGAGC CAATGATTCT CAGCACTCTA AATGTGATGT AGATAAGTCT 
GTGCAACCAG AGCCATTTTT CCATAAAGTG GTTCATTCTG AACACTTGAA CTTAGTCCCT 
CAAGTTCAAT CAGTTCAGTG TTCACCAGAA GAATCCTTTG CATTTCGATC TCACTCGCAT 
TTACCACCAA AAAATAAAAA CAAGAATTCC TTGCTGATTG GACTTTCAAC TGGTCTGTTT 
GATGCAAACA ACCCAAAGAT GTTAAGGACA TGTTCACTTC CAGATCTCTC AAAGCTGTTC 
AGAACCCTTA TGGATGTTCC CACCGTAGGA GATGTTCGTC AAGACAATCT TGAAATAGAT 
GAAATTGAAG ATGAAAACAT TAAAGAAGGA CCTTCTGATT CTGAAGACAT TGTGTTTGAA 
GAAACTGACA CAGATTTACA AGAGCTGCAG GCCTCAATGG AACAGTTACT TAGGGAACAA 
CCTAGTGAAG AATACAGTGA AGAAGAAGAG TCAGTCTTAA AGAACAGTGA TGTGGAGCCA 
ACTGCAAATG GGACAGATGT GGCGGATGAA GATGACAATC CCAGCAGTGA AAGTGCCCTG 
AACGAAGAAT GGCACTCAGA TAACAGTGAT GGGGAAAATG CTAGTGAATG TGAATACGAT 
AGTGTCTTTA ACCATTTAGA GGAACTGAGA CTTCATCTGG AGCAGGAAAT GGGCTTTGAA 
AAATTCTTTG AGGTTTACGA GAAAATAAAG GCTATTCATG AAGATGAAGA TGAAAATATT 
GAAATTTGTT CAAAAATAGT TCAAAATATT TTGGGAAATG AACATCAGCA TCTTTATGCC 
AAGATTCTTC ATTTAGTCAT GGCAGATGGA GCCTACCAAG AAGATAATGA TGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014994978.2
CDS	650..4426
Translation	MEKYVRLQKIGEGSFGKAILVKSTEDGRQYVIKEINISRMSSKEREESRREVAVLANMKHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGVLFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYELCTLKHAFEAGSMKNLVLKIISGSFPPVSLHYSYDLRSLVSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTFSKFGSQPIPAKRPASGQNSISVMPAQKITKPAAKYGIPLTYKKYGEKKLYEKKPLQKHKQAHQTPEKRVNIGEERRKMSEEAARKRRLEFIEKEKKQKDQIISLMKAEQMKRQEKERLERINRAREQGWRNVLSAGGGGEVKAPFLGSGGTIAPSSFSSRGQYEHYHAIFDQMQQQRAEDNEAKWKREIYGRGLPESGILPGVRPGFPYGAAGHHHFPDADDIRKTLKRLKAVSKQANANRQKGQLAVERAKQVEEFLQRKREAMQNKARAEGHMVYLARLRQIRLQNFNERQQIKAKLRGEKKEANRSEGQEGSEEADMRRKKIESLKAHANARAAVLKEQLERKRKEAYEREKKVWEEHLVAKGVKSSDVSPPLGQHETGGSPSKQQMRSVISVTSALKEVGVDSSLTDIREISEEMQKTNNAISSKREILRRLNENLKAQEDEKGKQHLSDTCEINVLEDAKEHEKEKSVSSDRKKWEAGGQLVIPLDELTLDTSFSTTERHTVGEVIKLDPNGSPRRVWGKSPTDSVLKILGEAELQLQTELLENTTIRSDISPEGEKYKPLITEEKKVQCISHEINPSAIVDSPVETKSPEFTEASPQMSLKLEGNLEEPDDLETEVLQEPSGTNKDESLPCTVTDVWISEEKEAKETQLEDRITIQENEVSEDGVSSTVDQLSDIHIETGANDSQHSKCDVDKSVQPEPFFHKVVHSEHLNLVPQVQSVQCSPEESFAFRSHSHLPPKNKNKNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDVRQDNLEIDEIEDENIKEGPSDSEDIVFEETDTDLQELQASMEQLLREQPSEEYSEEEESVLKNSDVEPTANGTDVADEDDNPSSESALNEEWHSDNSDGENASECEYDSVFNHLEELRLHLEQEMGFEKFFEVYEKIKAIHEDEDENIEICSKIVQNILGNEHQHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_015139492.2
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta NIMA related kinase 1 (NEK1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015139492.2

ORF Insert Sequence:

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG CAGACAGTAT GTTATCAAGG AAATTAACAT CTCAAGAATG 
TCCAGTAAAG AAAGAGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GTTCTCTCTA CATAGTGATG 
GATTACTGTG AGGGAGGGGA TCTGTTTAAG CGAATAAATG CTCAGAAAGG CGTTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTGAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATTAAA TCTCAGAACA TATTTTTAAC TAAAGATGGA 
ACGGTACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AATAATAAAA GTGACATTTG GGCTCTGGGG TGTGTCCTTT ATGAGCTGTG TACACTTAAA 
CATGCTTTTG AAGCTGGCAG TATGAAAAAC TTGGTACTGA AGATAATATC CGGATCTTTT 
CCACCTGTGT CTTTGCATTA TTCCTATGAT CTCCGCAGTT TGGTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC ACCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACGTTT 
TCAAAGTTTG GATCACAGCC TATACCAGCC AAAAGACCAG CTTCAGGACA AAACTCGATT 
TCTGTTATGC CTGCTCAGAA AATTACAAAG CCTGCCGCTA AATATGGAAT ACCTTTAACA 
TATAAGAAAT ATGGAGAGAA AAAATTATAC GAAAAGAAAC CACTGCAAAA ACATAAACAG 
GCCCATCAAA CTCCAGAGAA GAGAGTGAAT ATTGGAGAAG AAAGGAGGAA AATGTCTGAG 
GAAGCAGCAA GAAAGAGAAG GCTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTATTAGTT TAATGAAGGC TGAACAAATG AAAAGGCAAG AAAAGGAAAG GTTGGAAAGA 
ATAAATAGGG CCAGGGAACA AGGATGGAGA AATGTGCTAA GTGCTGGTGG AGGTGGTGAA 
GTAAAGGCTC CTTTTCTTGG CAGTGGAGGG ACTATAGCTC CATCATCTTT TTCTTCTCGA 
GGACAGTATG AACATTACCA TGCCATTTTT GACCAAATGC AACAACAGAG AGCAGAAGAT 
AATGAAGCTA AATGGAAAAG AGAAATATAT GGTCGAGGTC TTCCAGAAAG CGGAATTCTG 
CCTGGAGTTC GTCCAGGATT TCCTTATGGG GCTGCAGGTC ATCACCATTT CCCTGATGCT 
GATGATATTA GAAAAACTTT GAAAAGATTG AAGGCGGTGT CTAAACAAGC CAATGCAAAC 
AGGCAAAAAG GGCAGCTAGC TGTAGAAAGA GCTAAACAAG TAGAAGAGTT CCTGCAGCGA 
AAACGGGAAG CTATGCAGAA TAAAGCTCGA GCCGAAGGAC ATATGGTTTA TCTGGCAAGA 
CTGAGGCAAA TAAGACTACA GAATTTCAAT GAACGCCAAC AGATTAAAGC CAAACTTCGT 
GGTGAAAAGA AAGAAGCTAA TCGTTCTGAA GGACAAGAAG GAAGTGAAGA GGCTGACATG 
AGGCGCAAAA AAATCGAATC CCTGAAGGCC CATGCAAATG CACGTGCTGC TGTACTAAAA 
GAACAACTAG AACGAAAGAG AAAGGAAGCT TATGAGAGAG AAAAAAAAGT GTGGGAAGAG 
CATTTGGTGG CTAAAGGAGT TAAGAGTTCT GATGTTTCTC CACCTTTGGG ACAGCATGAA 
ACAGGTGGCT CTCCATCAAA GCAACAGATG AGATCGGTTA TTTCTGTGAC TTCAGCTTTG 
AAAGAAGTTG GCGTGGACAG TAGTTTAACT GATATCCGGG AAATTTCAGA AGAAATGCAA 
AAGACCAACA ATGCTATTTC AAGTAAGCGA GAAATACTTC GTAGATTAAA TGAAAATCTT 
AAAGCTCAAG AAGATGAAAA AGGAAAGCAG CATCTCTCTG ATACTTGTGA GATAAATGTT 
CTTGAAGATG CCAAAGAGCA TGAAAAAGAA AAATCAGTTT CATCTGATCG CAAGAAGTGG 
GAGGCAGGAG GTCAACTTGT GATTCCTCTG GATGAGTTAA CACTAGATAC ATCCTTCTCT 
ACAACTGAAA GACATACAGT GGGAGAAGTT ATTAAATTAG ATCCTAATGG ATCTCCAAGA 
AGAGTCTGGG GGAAAAGTCC GACAGATTCT GTTCTGAAGA TACTTGGAGA AGCTGAACTA 
CAACTTCAGA CAGAACTATT AGAAAATACA ACTATTAGAA GTGATATTTC TCCTGAAGGG 
GAAAAGTACA AACCCTTAAT TACTGAAGAA AAAAAAGTGC AATGTATTTC ACATGAAATA 
AACCCATCAG CTATTGTTGA TTCTCCTGTT GAGACAAAAA GCCCCGAGTT TACTGAGGCA 
TCTCCACAGA TGTCATTGAA ACTGGAAGGA AATTTAGAAG AACCTGATGA TTTGGAAACA 
GAAGTTCTAC AAGAGCCAAG TGGAACAAAC AAAGATGAGA GCTTGCCATG CACTGTTACT 
GATGTGTGGA TTAGTGAGGA AAAAGAAGCA AAGGAAACTC AGTTGGAAGA TAGGATCACC 
ATTCAAGAAA ATGAAGTTTC TGAGGATGGA GTCTCGAGTA CTGTGGACCA ACTTAGTGAC 
ATTCATATCG AGACGGGAGC CAATGATTCT CAGCACTCTA AATGTGATGT AGATAAGTCT 
GTGCAACCAG AGCCATTTTT CCATAAAGTG GTTCATTCTG AACACTTGAA CTTAGTCCCT 
CAAGTTCAAT CAGTTCAGTG TTCACCAGAA GAATCCTTTG CATTTCGATC TCACTCGCAT 
TTACCACCAA AAAATAAAAA CAAGAATTCC TTGCTGATTG GACTTTCAAC TGGTCTGTTT 
GATGCAAACA ACCCAAAGAT GTTAAGGACA TGTTCACTTC CAGATCTCTC AAAGCTGTTC 
AGAACCCTTA TGGATGTTCC CACCGTAGGA GATGTTCGTC AAGACAATCT TGAAATAGAT 
GAAATTGAAG ATGAAAACAT TAAAGAAGGA CCTTCTGATT CTGAAGACAT TGTGTTTGAA 
GAAACTGACA CAGATTTACA AGAGCTGCAG GCCTCAATGG AACAGTTACT TAGGGAACAA 
CCTAGTGAAG AATACAGTGA AGAAGAAGAG TCAGTCTTAA AGAACAGTGA TGTGGAGCCA 
ACTGCAAATG GGACAGATGT GGCGGATGAA GATGACAATC CCAGCAGTGA AAGTGCCCTG 
AACGAAGAAT GGCACTCAGA TAACAGTGAT GGGGAAAATG CTAGTGAATG TGAATACGAT 
AGTGTCTTTA ACCATTTAGA GGAACTGAGA CTTCATCTGG AGCAGGAAAT GGGCTTTGAA 
AAATTCTTTG AGGTTTACGA GAAAATAAAG GCTATTCATG AAGATGAAGA TGAAAATATT 
GAAATTTGTT CAAAAATAGT TCAAAATATT TTGGGAAATG AACATCAGCA TCTTTATGCC 
AAGATTCTTC ATTTAGTCAT GGCAGATGGA GCCTACCAAG AAGATAATGA TGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_028848884.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_028704717.1 Macaca mulatta NIMA related kinase 1 (NEK1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OMb86674
Clone ID Related Accession (Same CDS sequence)	XM_028848884.1
Accession Version	XM_028848884.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3735bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-25
Organism	Macaca mulatta(Rhesus monkey)
Product	serine/threonine-protein kinase Nek1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041758.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Macaca mulatta Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG CAGACAGTAT GTTATCAAGG AAATTAACAT CTCAAGAATG 
TCCAGTAAAG AAAGAGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GTTCTCTCTA CATAGTGATG 
GATTACTGTG AGGGAGGGGA TCTGTTTAAG CGAATAAATG CTCAGAAAGG CGTTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTGAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATTAAA TCTCAGAACA TATTTTTAAC TAAAGATGGA 
ACGGTACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AATAATAAAA GTGACATTTG GGCTCTGGGG TGTGTCCTTT ATGAGCTGTG TACACTTAAA 
CATGCTTTTG AAGCTGGCAG TATGAAAAAC TTGGTACTGA AGATAATATC CGGATCTTTT 
CCACCTGTGT CTTTGCATTA TTCCTATGAT CTCCGCAGTT TGGTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC ACCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACGTTT 
TCAAAGTTTG GATCACAGCC TATACCAGCC AAAAGACCAG CTTCAGGACA AAACTCGATT 
TCTGTTATGC CTGCTCAGAA AATTACAAAG CCTGCCGCTA AATATGGAAT ACCTTTAACA 
TATAAGAAAT ATGGAGAGAA AAAATTATAC GAAAAGAAAC CACTGCAAAA ACATAAACAG 
GCCCATCAAA CTCCAGAGAA GAGAGTGAAT ATTGGAGAAG AAAGGAGGAA AATGTCTGAG 
GAAGCAGCAA GAAAGAGAAG GCTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTATTAGTT TAATGAAGGC TGAACAAATG AAAAGGCAAG AAAAGGAAAG GTTGGAAAGA 
ATAAATAGGG CCAGGGAACA AGGATGGAGA AATGTGCTAA GTGCTGGTGG AGGTGGTGAA 
GTAAAGGCTC CTTTTCTTGG CAGTGGAGGG ACTATAGCTC CATCATCTTT TTCTTCTCGA 
GGACAGTATG AACATTACCA TGCCATTTTT GACCAAATGC AACAACAGAG AGCAGAAGAT 
AATGAAGCTA AATGGAAAAG AGAAATATAT GGTCGAGGTC TTCCAGAAAG GCAAAAAGGG 
CAGCTAGCTG TAGAAAGAGC TAAACAAGTA GAAGAGTTCC TGCAGCGAAA ACGGGAAGCT 
ATGCAGAATA AAGCTCGAGC CGAAGGACAT ATGGTTTATC TGGCAAGACT GAGGCAAATA 
AGACTACAGA ATTTCAATGA ACGCCAACAG ATTAAAGCCA AACTTCGTGG TGAAAAGAAA 
GAAGCTAATC GTTCTGAAGG ACAAGAAGGA AGTGAAGAGG CTGACATGAG GCGCAAAAAA 
ATCGAATCCC TGAAGGCCCA TGCAAATGCA CGTGCTGCTG TACTAAAAGA ACAACTAGAA 
CGAAAGAGAA AGGAAGCTTA TGAGAGAGAA AAAAAAGTGT GGGAAGAGCA TTTGGTGGCT 
AAAGGAGTTA AGAGTTCTGA TGTTTCTCCA CCTTTGGGAC AGCATGAAAC AGGTGGCTCT 
CCATCAAAGC AACAGATGAG ATCGGTTATT TCTGTGACTT CAGCTTTGAA AGAAGTTGGC 
GTGGACAGTA GTTTAACTGA TATCCGGGAA ATTTCAGAAG AAATGCAAAA GACCAACAAT 
GCTATTTCAA GTAAGCGAGA AATACTTCGT AGATTAAATG AAAATCTTAA AGCTCAAGAA 
GATGAAAAAG GAAAGCAGCA TCTCTCTGAT ACTTGTGAGA TAAATGTTCT TGAAGATGCC 
AAAGAGCATG AAAAAGAAAA ATCAGTTTCA TCTGATCGCA AGAAGTGGGA GGCAGGAGGT 
CAACTTGTGA TTCCTCTGGA TGAGTTAACA CTAGATACAT CCTTCTCTAC AACTGAAAGA 
CATACAGTGG GAGAAGTTAT TAAATTAGAT CCTAATGGAT CTCCAAGAAG AGTCTGGGGG 
AAAAGTCCGA CAGATTCTGT TCTGAAGATA CTTGGAGAAG CTGAACTACA ACTTCAGACA 
GAACTATTAG AAAATACAAC TATTAGAAGT GATATTTCTC CTGAAGGGGA AAAGTACAAA 
CCCTTAATTA CTGAAGAAAA AAAAGTGCAA TGTATTTCAC ATGAAATAAA CCCATCAGCT 
ATTGTTGATT CTCCTGTTGA GACAAAAAGC CCCGAGTTTA CTGAGGCATC TCCACAGATG 
TCATTGAAAC TGGAAGGAAA TTTAGAAGAA CCTGATGATT TGGAAACAGA AGTTCTACAA 
GAGCCAAGTG GAACAAACAA AGATGAGAGC TTGCCATGCA CTGTTACTGA TGTGTGGATT 
AGTGAGGAAA AAGAAGCAAA GGAAACTCAG TTGGAAGATA GGATCACCAT TCAAGAAAAT 
GAAGTTTCTG AGGATGGAGT CTCGAGTACT GTGGACCAAC TTAGTGACAT TCATATCGAG 
ACGGGAGCCA ATGATTCTCA GCACTCTAAA TGTGATGTAG ATAAGTCTGT GCAACCAGAG 
CCATTTTTCC ATAAAGTGGT TCATTCTGAA CACTTGAACT TAGTCCCTCA AGTTCAATCA 
GTTCAGTGTT CACCAGAAGA ATCCTTTGCA TTTCGATCTC ACTCGCATTT ACCACCAAAA 
AATAAAAACA AGAATTCCTT GCTGATTGGA CTTTCAACTG GTCTGTTTGA TGCAAACAAC 
CCAAAGATGT TAAGGACATG TTCACTTCCA GATCTCTCAA AGCTGTTCAG AACCCTTATG 
GATGTTCCCA CCGTAGGAGA TGTTCGTCAA GACAATCTTG AAATAGATGA AATTGAAGAT 
GAAAACATTA AAGAAGGACC TTCTGATTCT GAAGACATTG TGTTTGAAGA AACTGACACA 
GATTTACAAG AGCTGCAGGC CTCAATGGAA CAGTTACTTA GGGAACAACC TAGTGAAGAA 
TACAGTGAAG AAGAAGAGTC AGTCTTAAAG AACAGTGATG TGGAGCCAAC TGCAAATGGG 
ACAGATGTGG CGGATGAAGA TGACAATCCC AGCAGTGAAA GTGCCCTGAA CGAAGAATGG 
CACTCAGATA ACAGTGATGG GGAAAATGCT AGTGAATGTG AATACGATAG TGTCTTTAAC 
CATTTAGAGG AACTGAGACT TCATCTGGAG CAGGAAATGG GCTTTGAAAA ATTCTTTGAG 
GTTTACGAGA AAATAAAGGC TATTCATGAA GATGAAGATG AAAATATTGA AATTTGTTCA 
AAAATAGTTC AAAATATTTT GGGAAATGAA CATCAGCATC TTTATGCCAA GATTCTTCAT 
TTAGTCATGG CAGATGGAGC CTACCAAGAA GATGATTGTC CTGCCAGACC TCTATTGAGC 
TGGATAGGGA TGGCACCATG TTCAAGAGGC CAAAGAAGAG ACCTGGAGCC AGCAAACAGA 
CACAAGGTTT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028704717.1
CDS	632..4366
Translation	MEKYVRLQKIGEGSFGKAILVKSTEDGRQYVIKEINISRMSSKEREESRREVAVLANMKHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGVLFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYELCTLKHAFEAGSMKNLVLKIISGSFPPVSLHYSYDLRSLVSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTFSKFGSQPIPAKRPASGQNSISVMPAQKITKPAAKYGIPLTYKKYGEKKLYEKKPLQKHKQAHQTPEKRVNIGEERRKMSEEAARKRRLEFIEKEKKQKDQIISLMKAEQMKRQEKERLERINRAREQGWRNVLSAGGGGEVKAPFLGSGGTIAPSSFSSRGQYEHYHAIFDQMQQQRAEDNEAKWKREIYGRGLPERQKGQLAVERAKQVEEFLQRKREAMQNKARAEGHMVYLARLRQIRLQNFNERQQIKAKLRGEKKEANRSEGQEGSEEADMRRKKIESLKAHANARAAVLKEQLERKRKEAYEREKKVWEEHLVAKGVKSSDVSPPLGQHETGGSPSKQQMRSVISVTSALKEVGVDSSLTDIREISEEMQKTNNAISSKREILRRLNENLKAQEDEKGKQHLSDTCEINVLEDAKEHEKEKSVSSDRKKWEAGGQLVIPLDELTLDTSFSTTERHTVGEVIKLDPNGSPRRVWGKSPTDSVLKILGEAELQLQTELLENTTIRSDISPEGEKYKPLITEEKKVQCISHEINPSAIVDSPVETKSPEFTEASPQMSLKLEGNLEEPDDLETEVLQEPSGTNKDESLPCTVTDVWISEEKEAKETQLEDRITIQENEVSEDGVSSTVDQLSDIHIETGANDSQHSKCDVDKSVQPEPFFHKVVHSEHLNLVPQVQSVQCSPEESFAFRSHSHLPPKNKNKNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDVRQDNLEIDEIEDENIKEGPSDSEDIVFEETDTDLQELQASMEQLLREQPSEEYSEEEESVLKNSDVEPTANGTDVADEDDNPSSESALNEEWHSDNSDGENASECEYDSVFNHLEELRLHLEQEMGFEKFFEVYEKIKAIHEDEDENIEICSKIVQNILGNEHQHLYAKILHLVMADGAYQEDDCPARPLLSWIGMAPCSRGQRRDLEPANRHKVY

Target ORF information:

RefSeq Version	XM_028848884.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta NIMA related kinase 1 (NEK1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_028848884.1

ORF Insert Sequence:

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG CAGACAGTAT GTTATCAAGG AAATTAACAT CTCAAGAATG 
TCCAGTAAAG AAAGAGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GTTCTCTCTA CATAGTGATG 
GATTACTGTG AGGGAGGGGA TCTGTTTAAG CGAATAAATG CTCAGAAAGG CGTTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTGAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATTAAA TCTCAGAACA TATTTTTAAC TAAAGATGGA 
ACGGTACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AATAATAAAA GTGACATTTG GGCTCTGGGG TGTGTCCTTT ATGAGCTGTG TACACTTAAA 
CATGCTTTTG AAGCTGGCAG TATGAAAAAC TTGGTACTGA AGATAATATC CGGATCTTTT 
CCACCTGTGT CTTTGCATTA TTCCTATGAT CTCCGCAGTT TGGTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC ACCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACGTTT 
TCAAAGTTTG GATCACAGCC TATACCAGCC AAAAGACCAG CTTCAGGACA AAACTCGATT 
TCTGTTATGC CTGCTCAGAA AATTACAAAG CCTGCCGCTA AATATGGAAT ACCTTTAACA 
TATAAGAAAT ATGGAGAGAA AAAATTATAC GAAAAGAAAC CACTGCAAAA ACATAAACAG 
GCCCATCAAA CTCCAGAGAA GAGAGTGAAT ATTGGAGAAG AAAGGAGGAA AATGTCTGAG 
GAAGCAGCAA GAAAGAGAAG GCTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTATTAGTT TAATGAAGGC TGAACAAATG AAAAGGCAAG AAAAGGAAAG GTTGGAAAGA 
ATAAATAGGG CCAGGGAACA AGGATGGAGA AATGTGCTAA GTGCTGGTGG AGGTGGTGAA 
GTAAAGGCTC CTTTTCTTGG CAGTGGAGGG ACTATAGCTC CATCATCTTT TTCTTCTCGA 
GGACAGTATG AACATTACCA TGCCATTTTT GACCAAATGC AACAACAGAG AGCAGAAGAT 
AATGAAGCTA AATGGAAAAG AGAAATATAT GGTCGAGGTC TTCCAGAAAG GCAAAAAGGG 
CAGCTAGCTG TAGAAAGAGC TAAACAAGTA GAAGAGTTCC TGCAGCGAAA ACGGGAAGCT 
ATGCAGAATA AAGCTCGAGC CGAAGGACAT ATGGTTTATC TGGCAAGACT GAGGCAAATA 
AGACTACAGA ATTTCAATGA ACGCCAACAG ATTAAAGCCA AACTTCGTGG TGAAAAGAAA 
GAAGCTAATC GTTCTGAAGG ACAAGAAGGA AGTGAAGAGG CTGACATGAG GCGCAAAAAA 
ATCGAATCCC TGAAGGCCCA TGCAAATGCA CGTGCTGCTG TACTAAAAGA ACAACTAGAA 
CGAAAGAGAA AGGAAGCTTA TGAGAGAGAA AAAAAAGTGT GGGAAGAGCA TTTGGTGGCT 
AAAGGAGTTA AGAGTTCTGA TGTTTCTCCA CCTTTGGGAC AGCATGAAAC AGGTGGCTCT 
CCATCAAAGC AACAGATGAG ATCGGTTATT TCTGTGACTT CAGCTTTGAA AGAAGTTGGC 
GTGGACAGTA GTTTAACTGA TATCCGGGAA ATTTCAGAAG AAATGCAAAA GACCAACAAT 
GCTATTTCAA GTAAGCGAGA AATACTTCGT AGATTAAATG AAAATCTTAA AGCTCAAGAA 
GATGAAAAAG GAAAGCAGCA TCTCTCTGAT ACTTGTGAGA TAAATGTTCT TGAAGATGCC 
AAAGAGCATG AAAAAGAAAA ATCAGTTTCA TCTGATCGCA AGAAGTGGGA GGCAGGAGGT 
CAACTTGTGA TTCCTCTGGA TGAGTTAACA CTAGATACAT CCTTCTCTAC AACTGAAAGA 
CATACAGTGG GAGAAGTTAT TAAATTAGAT CCTAATGGAT CTCCAAGAAG AGTCTGGGGG 
AAAAGTCCGA CAGATTCTGT TCTGAAGATA CTTGGAGAAG CTGAACTACA ACTTCAGACA 
GAACTATTAG AAAATACAAC TATTAGAAGT GATATTTCTC CTGAAGGGGA AAAGTACAAA 
CCCTTAATTA CTGAAGAAAA AAAAGTGCAA TGTATTTCAC ATGAAATAAA CCCATCAGCT 
ATTGTTGATT CTCCTGTTGA GACAAAAAGC CCCGAGTTTA CTGAGGCATC TCCACAGATG 
TCATTGAAAC TGGAAGGAAA TTTAGAAGAA CCTGATGATT TGGAAACAGA AGTTCTACAA 
GAGCCAAGTG GAACAAACAA AGATGAGAGC TTGCCATGCA CTGTTACTGA TGTGTGGATT 
AGTGAGGAAA AAGAAGCAAA GGAAACTCAG TTGGAAGATA GGATCACCAT TCAAGAAAAT 
GAAGTTTCTG AGGATGGAGT CTCGAGTACT GTGGACCAAC TTAGTGACAT TCATATCGAG 
ACGGGAGCCA ATGATTCTCA GCACTCTAAA TGTGATGTAG ATAAGTCTGT GCAACCAGAG 
CCATTTTTCC ATAAAGTGGT TCATTCTGAA CACTTGAACT TAGTCCCTCA AGTTCAATCA 
GTTCAGTGTT CACCAGAAGA ATCCTTTGCA TTTCGATCTC ACTCGCATTT ACCACCAAAA 
AATAAAAACA AGAATTCCTT GCTGATTGGA CTTTCAACTG GTCTGTTTGA TGCAAACAAC 
CCAAAGATGT TAAGGACATG TTCACTTCCA GATCTCTCAA AGCTGTTCAG AACCCTTATG 
GATGTTCCCA CCGTAGGAGA TGTTCGTCAA GACAATCTTG AAATAGATGA AATTGAAGAT 
GAAAACATTA AAGAAGGACC TTCTGATTCT GAAGACATTG TGTTTGAAGA AACTGACACA 
GATTTACAAG AGCTGCAGGC CTCAATGGAA CAGTTACTTA GGGAACAACC TAGTGAAGAA 
TACAGTGAAG AAGAAGAGTC AGTCTTAAAG AACAGTGATG TGGAGCCAAC TGCAAATGGG 
ACAGATGTGG CGGATGAAGA TGACAATCCC AGCAGTGAAA GTGCCCTGAA CGAAGAATGG 
CACTCAGATA ACAGTGATGG GGAAAATGCT AGTGAATGTG AATACGATAG TGTCTTTAAC 
CATTTAGAGG AACTGAGACT TCATCTGGAG CAGGAAATGG GCTTTGAAAA ATTCTTTGAG 
GTTTACGAGA AAATAAAGGC TATTCATGAA GATGAAGATG AAAATATTGA AATTTGTTCA 
AAAATAGTTC AAAATATTTT GGGAAATGAA CATCAGCATC TTTATGCCAA GATTCTTCAT 
TTAGTCATGG CAGATGGAGC CTACCAAGAA GATGATTGTC CTGCCAGACC TCTATTGAGC 
TGGATAGGGA TGGCACCATG TTCAAGAGGC CAAAGAAGAG ACCTGGAGCC AGCAAACAGA 
CACAAGGTTT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_001083043.4 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_001083043.3 Macaca mulatta NIMA related kinase 1 (NEK1), transcript variant X9, mRNA.

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CloneID	OMb86675
Clone ID Related Accession (Same CDS sequence)	XM_001083043.4
Accession Version	XM_001083043.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3729bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-25
Organism	Macaca mulatta(Rhesus monkey)
Product	serine/threonine-protein kinase Nek1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041758.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 23, 2019 this sequence version replaced XM_001083043.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Macaca mulatta Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG CAGACAGTAT GTTATCAAGG AAATTAACAT CTCAAGAATG 
TCCAGTAAAG AAAGAGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GTTCTCTCTA CATAGTGATG 
GATTACTGTG AGGGAGGGGA TCTGTTTAAG CGAATAAATG CTCAGAAAGG CGTTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTGAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATTAAA TCTCAGAACA TATTTTTAAC TAAAGATGGA 
ACGGTACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AATAATAAAA GTGACATTTG GGCTCTGGGG TGTGTCCTTT ATGAGCTGTG TACACTTAAA 
CATGCTTTTG AAGCTGGCAG TATGAAAAAC TTGGTACTGA AGATAATATC CGGATCTTTT 
CCACCTGTGT CTTTGCATTA TTCCTATGAT CTCCGCAGTT TGGTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC ACCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACGTTT 
TCAAAGTTTG GATCACAGCC TATACCAGCC AAAAGACCAG CTTCAGGACA AAACTCGATT 
TCTGTTATGC CTGCTCAGAA AATTACAAAG CCTGCCGCTA AATATGGAAT ACCTTTAACA 
TATAAGAAAT ATGGAGAGAA AAAATTATAC GAAAAGAAAC CACTGCAAAA ACATAAACAG 
GCCCATCAAA CTCCAGAGAA GAGAGTGAAT ATTGGAGAAG AAAGGAGGAA AATGTCTGAG 
GAAGCAGCAA GAAAGAGAAG GCTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTATTAGTT TAATGAAGGC TGAACAAATG AAAAGGCAAG AAAAGGAAAG GTTGGAAAGA 
ATAAATAGGG CCAGGGAACA AGGATGGAGA AATGTGCTAA GTGCTGGTGG AGGTGGTGAA 
GTAAAGGCTC CTTTTCTTGG CAGTGGAGGG ACTATAGCTC CATCATCTTT TTCTTCTCGA 
GGACAGTATG AACATTACCA TGCCATTTTT GACCAAATGC AACAACAGAG AGCAGAAGAT 
AATGAAGCTA AATGGAAAAG AGAAATATAT GGTCGAGGTC TTCCAGAAAG GCAAAAAGGG 
CAGCTAGCTG TAGAAAGAGC TAAACAAGTA GAAGAGTTCC TGCAGCGAAA ACGGGAAGCT 
ATGCAGAATA AAGCTCGAGC CGAAGGACAT ATGGGAATCC TGCAAAACCT GGCAGCTATG 
TATGGAGGCA GGCCCAGCTC TTCAAGAGGA GGGAAGCCAA GGAACAAAGA GGAAGAGGTT 
TATCTGGCAA GACTGAGGCA AATAAGACTA CAGAATTTCA ATGAACGCCA ACAGATTAAA 
GCCAAACTTC GTGGTGAAAA GAAAGAAGCT AATCGTTCTG AAGGACAAGA AGGAAGTGAA 
GAGGCTGACA TGAGGCGCAA AAAAATCGAA TCCCTGAAGG CCCATGCAAA TGCACGTGCT 
GCTGTACTAA AAGAACAACT AGAACGAAAG AGAAAGGAAG CTTATGAGAG AGAAAAAAAA 
GTGTGGGAAG AGCATTTGGT GGCTAAAGGA GTTAAGAGTT CTGATGTTTC TCCACCTTTG 
GGACAGCATG AAACAGGTGG CTCTCCATCA AAGCAACAGA TGAGATCGGT TATTTCTGTG 
ACTTCAGCTT TGAAAGAAGT TGGCGTGGAC AGTAGTTTAA CTGATATCCG GGAAATTTCA 
GAAGAAATGC AAAAGACCAA CAATGCTATT TCAAGTAAGC GAGAAATACT TCGTAGATTA 
AATGAAAATC TTAAAGCTCA AGAAGATGAA AAAGGAAAGC AGCATCTCTC TGATACTTGT 
GAGATAAATG TTCTTGAAGA TGCCAAAGAG CATGAAAAAG AAAAATCAGT TTCATCTGAT 
CGCAAGAAGT GGGAGGCAGG AGGTCAACTT GTGATTCCTC TGGATGAGTT AACACTAGAT 
ACATCCTTCT CTACAACTGA AAGACATACA GTGGGAGAAG TTATTAAATT AGATCCTAAT 
GGATCTCCAA GAAGAGTCTG GGGGAAAAGT CCGACAGATT CTGTTCTGAA GATACTTGGA 
GAAGCTGAAC TACAACTTCA GACAGAACTA TTAGAAAATA CAACTATTAG AAGTGATATT 
TCTCCTGAAG GGGAAAAGTA CAAACCCTTA ATTACTGAAG AAAAAAAAGT GCAATGTATT 
TCACATGAAA TAAACCCATC AGCTATTGTT GATTCTCCTG TTGAGACAAA AAGCCCCGAG 
TTTACTGAGG CATCTCCACA GATGTCATTG AAACTGGAAG GAAATTTAGA AGAACCTGAT 
GATTTGGAAA CAGAAGTTCT ACAAGAGCCA AGTGGAACAA ACAAAGATGA GAGCTTGCCA 
TGCACTGTTA CTGATGTGTG GATTAGTGAG GAAAAAGAAG CAAAGGAAAC TCAGTTGGAA 
GATAGGATCA CCATTCAAGA AAATGAAGTT TCTGAGGATG GAGTCTCGAG TACTGTGGAC 
CAACTTAGTG ACATTCATAT CGAGACGGGA GCCAATGATT CTCAGCACTC TAAATGTGAT 
GTAGATAAGT CTGTGCAACC AGAGCCATTT TTCCATAAAG TGGTTCATTC TGAACACTTG 
AACTTAGTCC CTCAAGTTCA ATCAGTTCAG TGTTCACCAG AAGAATCCTT TGCATTTCGA 
TCTCACTCGC ATTTACCACC AAAAAATAAA AACAAGAATT CCTTGCTGAT TGGACTTTCA 
ACTGGTCTGT TTGATGCAAA CAACCCAAAG ATGTTAAGGA CATGTTCACT TCCAGATCTC 
TCAAAGCTGT TCAGAACCCT TATGGATGTT CCCACCGTAG GAGATGTTCG TCAAGACAAT 
CTTGAAATAG ATGAAATTGA AGATGAAAAC ATTAAAGAAG GACCTTCTGA TTCTGAAGAC 
ATTGTGTTTG AAGAAACTGA CACAGATTTA CAAGAGCTGC AGGCCTCAAT GGAACAGTTA 
CTTAGGGAAC AACCTAGTGA AGAATACAGT GAAGAAGAAG AGTCAGTCTT AAAGAACAGT 
GATGTGGAGC CAACTGCAAA TGGGACAGAT GTGGCGGATG AAGATGACAA TCCCAGCAGT 
GAAAGTGCCC TGAACGAAGA ATGGCACTCA GATAACAGTG ATGGGGAAAA TGCTAGTGAA 
TGTGAATACG ATAGTGTCTT TAACCATTTA GAGGAACTGA GACTTCATCT GGAGCAGGAA 
ATGGGCTTTG AAAAATTCTT TGAGGTTTAC GAGAAAATAA AGGCTATTCA TGAAGATGAA 
GATGAAAATA TTGAAATTTG TTCAAAAATA GTTCAAAATA TTTTGGGAAA TGAACATCAG 
CATCTTTATG CCAAGATTCT TCATTTAGTC ATGGCAGATG GAGCCTACCA AGAAGATAAT 
GATGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001083043.3
CDS	575..4303
Translation	MEKYVRLQKIGEGSFGKAILVKSTEDGRQYVIKEINISRMSSKEREESRREVAVLANMKHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGVLFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYELCTLKHAFEAGSMKNLVLKIISGSFPPVSLHYSYDLRSLVSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTFSKFGSQPIPAKRPASGQNSISVMPAQKITKPAAKYGIPLTYKKYGEKKLYEKKPLQKHKQAHQTPEKRVNIGEERRKMSEEAARKRRLEFIEKEKKQKDQIISLMKAEQMKRQEKERLERINRAREQGWRNVLSAGGGGEVKAPFLGSGGTIAPSSFSSRGQYEHYHAIFDQMQQQRAEDNEAKWKREIYGRGLPERQKGQLAVERAKQVEEFLQRKREAMQNKARAEGHMGILQNLAAMYGGRPSSSRGGKPRNKEEEVYLARLRQIRLQNFNERQQIKAKLRGEKKEANRSEGQEGSEEADMRRKKIESLKAHANARAAVLKEQLERKRKEAYEREKKVWEEHLVAKGVKSSDVSPPLGQHETGGSPSKQQMRSVISVTSALKEVGVDSSLTDIREISEEMQKTNNAISSKREILRRLNENLKAQEDEKGKQHLSDTCEINVLEDAKEHEKEKSVSSDRKKWEAGGQLVIPLDELTLDTSFSTTERHTVGEVIKLDPNGSPRRVWGKSPTDSVLKILGEAELQLQTELLENTTIRSDISPEGEKYKPLITEEKKVQCISHEINPSAIVDSPVETKSPEFTEASPQMSLKLEGNLEEPDDLETEVLQEPSGTNKDESLPCTVTDVWISEEKEAKETQLEDRITIQENEVSEDGVSSTVDQLSDIHIETGANDSQHSKCDVDKSVQPEPFFHKVVHSEHLNLVPQVQSVQCSPEESFAFRSHSHLPPKNKNKNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDVRQDNLEIDEIEDENIKEGPSDSEDIVFEETDTDLQELQASMEQLLREQPSEEYSEEEESVLKNSDVEPTANGTDVADEDDNPSSESALNEEWHSDNSDGENASECEYDSVFNHLEELRLHLEQEMGFEKFFEVYEKIKAIHEDEDENIEICSKIVQNILGNEHQHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_001083043.4
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta NIMA related kinase 1 (NEK1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001083043.4

ORF Insert Sequence:

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG CAGACAGTAT GTTATCAAGG AAATTAACAT CTCAAGAATG 
TCCAGTAAAG AAAGAGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GTTCTCTCTA CATAGTGATG 
GATTACTGTG AGGGAGGGGA TCTGTTTAAG CGAATAAATG CTCAGAAAGG CGTTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTGAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATTAAA TCTCAGAACA TATTTTTAAC TAAAGATGGA 
ACGGTACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AATAATAAAA GTGACATTTG GGCTCTGGGG TGTGTCCTTT ATGAGCTGTG TACACTTAAA 
CATGCTTTTG AAGCTGGCAG TATGAAAAAC TTGGTACTGA AGATAATATC CGGATCTTTT 
CCACCTGTGT CTTTGCATTA TTCCTATGAT CTCCGCAGTT TGGTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC ACCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACGTTT 
TCAAAGTTTG GATCACAGCC TATACCAGCC AAAAGACCAG CTTCAGGACA AAACTCGATT 
TCTGTTATGC CTGCTCAGAA AATTACAAAG CCTGCCGCTA AATATGGAAT ACCTTTAACA 
TATAAGAAAT ATGGAGAGAA AAAATTATAC GAAAAGAAAC CACTGCAAAA ACATAAACAG 
GCCCATCAAA CTCCAGAGAA GAGAGTGAAT ATTGGAGAAG AAAGGAGGAA AATGTCTGAG 
GAAGCAGCAA GAAAGAGAAG GCTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTATTAGTT TAATGAAGGC TGAACAAATG AAAAGGCAAG AAAAGGAAAG GTTGGAAAGA 
ATAAATAGGG CCAGGGAACA AGGATGGAGA AATGTGCTAA GTGCTGGTGG AGGTGGTGAA 
GTAAAGGCTC CTTTTCTTGG CAGTGGAGGG ACTATAGCTC CATCATCTTT TTCTTCTCGA 
GGACAGTATG AACATTACCA TGCCATTTTT GACCAAATGC AACAACAGAG AGCAGAAGAT 
AATGAAGCTA AATGGAAAAG AGAAATATAT GGTCGAGGTC TTCCAGAAAG GCAAAAAGGG 
CAGCTAGCTG TAGAAAGAGC TAAACAAGTA GAAGAGTTCC TGCAGCGAAA ACGGGAAGCT 
ATGCAGAATA AAGCTCGAGC CGAAGGACAT ATGGGAATCC TGCAAAACCT GGCAGCTATG 
TATGGAGGCA GGCCCAGCTC TTCAAGAGGA GGGAAGCCAA GGAACAAAGA GGAAGAGGTT 
TATCTGGCAA GACTGAGGCA AATAAGACTA CAGAATTTCA ATGAACGCCA ACAGATTAAA 
GCCAAACTTC GTGGTGAAAA GAAAGAAGCT AATCGTTCTG AAGGACAAGA AGGAAGTGAA 
GAGGCTGACA TGAGGCGCAA AAAAATCGAA TCCCTGAAGG CCCATGCAAA TGCACGTGCT 
GCTGTACTAA AAGAACAACT AGAACGAAAG AGAAAGGAAG CTTATGAGAG AGAAAAAAAA 
GTGTGGGAAG AGCATTTGGT GGCTAAAGGA GTTAAGAGTT CTGATGTTTC TCCACCTTTG 
GGACAGCATG AAACAGGTGG CTCTCCATCA AAGCAACAGA TGAGATCGGT TATTTCTGTG 
ACTTCAGCTT TGAAAGAAGT TGGCGTGGAC AGTAGTTTAA CTGATATCCG GGAAATTTCA 
GAAGAAATGC AAAAGACCAA CAATGCTATT TCAAGTAAGC GAGAAATACT TCGTAGATTA 
AATGAAAATC TTAAAGCTCA AGAAGATGAA AAAGGAAAGC AGCATCTCTC TGATACTTGT 
GAGATAAATG TTCTTGAAGA TGCCAAAGAG CATGAAAAAG AAAAATCAGT TTCATCTGAT 
CGCAAGAAGT GGGAGGCAGG AGGTCAACTT GTGATTCCTC TGGATGAGTT AACACTAGAT 
ACATCCTTCT CTACAACTGA AAGACATACA GTGGGAGAAG TTATTAAATT AGATCCTAAT 
GGATCTCCAA GAAGAGTCTG GGGGAAAAGT CCGACAGATT CTGTTCTGAA GATACTTGGA 
GAAGCTGAAC TACAACTTCA GACAGAACTA TTAGAAAATA CAACTATTAG AAGTGATATT 
TCTCCTGAAG GGGAAAAGTA CAAACCCTTA ATTACTGAAG AAAAAAAAGT GCAATGTATT 
TCACATGAAA TAAACCCATC AGCTATTGTT GATTCTCCTG TTGAGACAAA AAGCCCCGAG 
TTTACTGAGG CATCTCCACA GATGTCATTG AAACTGGAAG GAAATTTAGA AGAACCTGAT 
GATTTGGAAA CAGAAGTTCT ACAAGAGCCA AGTGGAACAA ACAAAGATGA GAGCTTGCCA 
TGCACTGTTA CTGATGTGTG GATTAGTGAG GAAAAAGAAG CAAAGGAAAC TCAGTTGGAA 
GATAGGATCA CCATTCAAGA AAATGAAGTT TCTGAGGATG GAGTCTCGAG TACTGTGGAC 
CAACTTAGTG ACATTCATAT CGAGACGGGA GCCAATGATT CTCAGCACTC TAAATGTGAT 
GTAGATAAGT CTGTGCAACC AGAGCCATTT TTCCATAAAG TGGTTCATTC TGAACACTTG 
AACTTAGTCC CTCAAGTTCA ATCAGTTCAG TGTTCACCAG AAGAATCCTT TGCATTTCGA 
TCTCACTCGC ATTTACCACC AAAAAATAAA AACAAGAATT CCTTGCTGAT TGGACTTTCA 
ACTGGTCTGT TTGATGCAAA CAACCCAAAG ATGTTAAGGA CATGTTCACT TCCAGATCTC 
TCAAAGCTGT TCAGAACCCT TATGGATGTT CCCACCGTAG GAGATGTTCG TCAAGACAAT 
CTTGAAATAG ATGAAATTGA AGATGAAAAC ATTAAAGAAG GACCTTCTGA TTCTGAAGAC 
ATTGTGTTTG AAGAAACTGA CACAGATTTA CAAGAGCTGC AGGCCTCAAT GGAACAGTTA 
CTTAGGGAAC AACCTAGTGA AGAATACAGT GAAGAAGAAG AGTCAGTCTT AAAGAACAGT 
GATGTGGAGC CAACTGCAAA TGGGACAGAT GTGGCGGATG AAGATGACAA TCCCAGCAGT 
GAAAGTGCCC TGAACGAAGA ATGGCACTCA GATAACAGTG ATGGGGAAAA TGCTAGTGAA 
TGTGAATACG ATAGTGTCTT TAACCATTTA GAGGAACTGA GACTTCATCT GGAGCAGGAA 
ATGGGCTTTG AAAAATTCTT TGAGGTTTAC GAGAAAATAA AGGCTATTCA TGAAGATGAA 
GATGAAAATA TTGAAATTTG TTCAAAAATA GTTCAAAATA TTTTGGGAAA TGAACATCAG 
CATCTTTATG CCAAGATTCT TCATTTAGTC ATGGCAGATG GAGCCTACCA AGAAGATAAT 
GATGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_015139494.2 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_014994980.2 Macaca mulatta NIMA related kinase 1 (NEK1), transcript variant X10, mRNA.

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CloneID	OMb86676
Clone ID Related Accession (Same CDS sequence)	XM_015139494.2
Accession Version	XM_015139494.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3645bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-25
Organism	Macaca mulatta(Rhesus monkey)
Product	serine/threonine-protein kinase Nek1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041758.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 23, 2019 this sequence version replaced XM_015139494.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Macaca mulatta Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG CAGACAGTAT GTTATCAAGG AAATTAACAT CTCAAGAATG 
TCCAGTAAAG AAAGAGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GTTCTCTCTA CATAGTGATG 
GATTACTGTG AGGGAGGGGA TCTGTTTAAG CGAATAAATG CTCAGAAAGG CGTTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTGAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATTAAA TCTCAGAACA TATTTTTAAC TAAAGATGGA 
ACGGTACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AATAATAAAA GTGACATTTG GGCTCTGGGG TGTGTCCTTT ATGAGCTGTG TACACTTAAA 
CATGCTTTTG AAGCTGGCAG TATGAAAAAC TTGGTACTGA AGATAATATC CGGATCTTTT 
CCACCTGTGT CTTTGCATTA TTCCTATGAT CTCCGCAGTT TGGTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC ACCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACGTTT 
TCAAAGTTTG GATCACAGCC TATACCAGCC AAAAGACCAG CTTCAGGACA AAACTCGATT 
TCTGTTATGC CTGCTCAGAA AATTACAAAG CCTGCCGCTA AATATGGAAT ACCTTTAACA 
TATAAGAAAT ATGGAGAGAA AAAATTATAC GAAAAGAAAC CACTGCAAAA ACATAAACAG 
GCCCATCAAA CTCCAGAGAA GAGAGTGAAT ATTGGAGAAG AAAGGAGGAA AATGTCTGAG 
GAAGCAGCAA GAAAGAGAAG GCTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTATTAGTT TAATGAAGGC TGAACAAATG AAAAGGCAAG AAAAGGAAAG GTTGGAAAGA 
ATAAATAGGG CCAGGGAACA AGGATGGAGA AATGTGCTAA GTGCTGGTGG AGGTGGTGAA 
GTAAAGGCTC CTTTTCTTGG CAGTGGAGGG ACTATAGCTC CATCATCTTT TTCTTCTCGA 
GGACAGTATG AACATTACCA TGCCATTTTT GACCAAATGC AACAACAGAG AGCAGAAGAT 
AATGAAGCTA AATGGAAAAG AGAAATATAT GGTCGAGGTC TTCCAGAAAG GCAAAAAGGG 
CAGCTAGCTG TAGAAAGAGC TAAACAAGTA GAAGAGTTCC TGCAGCGAAA ACGGGAAGCT 
ATGCAGAATA AAGCTCGAGC CGAAGGACAT ATGGTTTATC TGGCAAGACT GAGGCAAATA 
AGACTACAGA ATTTCAATGA ACGCCAACAG ATTAAAGCCA AACTTCGTGG TGAAAAGAAA 
GAAGCTAATC GTTCTGAAGG ACAAGAAGGA AGTGAAGAGG CTGACATGAG GCGCAAAAAA 
ATCGAATCCC TGAAGGCCCA TGCAAATGCA CGTGCTGCTG TACTAAAAGA ACAACTAGAA 
CGAAAGAGAA AGGAAGCTTA TGAGAGAGAA AAAAAAGTGT GGGAAGAGCA TTTGGTGGCT 
AAAGGAGTTA AGAGTTCTGA TGTTTCTCCA CCTTTGGGAC AGCATGAAAC AGGTGGCTCT 
CCATCAAAGC AACAGATGAG ATCGGTTATT TCTGTGACTT CAGCTTTGAA AGAAGTTGGC 
GTGGACAGTA GTTTAACTGA TATCCGGGAA ATTTCAGAAG AAATGCAAAA GACCAACAAT 
GCTATTTCAA GTAAGCGAGA AATACTTCGT AGATTAAATG AAAATCTTAA AGCTCAAGAA 
GATGAAAAAG GAAAGCAGCA TCTCTCTGAT ACTTGTGAGA TAAATGTTCT TGAAGATGCC 
AAAGAGCATG AAAAAGAAAA ATCAGTTTCA TCTGATCGCA AGAAGTGGGA GGCAGGAGGT 
CAACTTGTGA TTCCTCTGGA TGAGTTAACA CTAGATACAT CCTTCTCTAC AACTGAAAGA 
CATACAGTGG GAGAAGTTAT TAAATTAGAT CCTAATGGAT CTCCAAGAAG AGTCTGGGGG 
AAAAGTCCGA CAGATTCTGT TCTGAAGATA CTTGGAGAAG CTGAACTACA ACTTCAGACA 
GAACTATTAG AAAATACAAC TATTAGAAGT GATATTTCTC CTGAAGGGGA AAAGTACAAA 
CCCTTAATTA CTGAAGAAAA AAAAGTGCAA TGTATTTCAC ATGAAATAAA CCCATCAGCT 
ATTGTTGATT CTCCTGTTGA GACAAAAAGC CCCGAGTTTA CTGAGGCATC TCCACAGATG 
TCATTGAAAC TGGAAGGAAA TTTAGAAGAA CCTGATGATT TGGAAACAGA AGTTCTACAA 
GAGCCAAGTG GAACAAACAA AGATGAGAGC TTGCCATGCA CTGTTACTGA TGTGTGGATT 
AGTGAGGAAA AAGAAGCAAA GGAAACTCAG TTGGAAGATA GGATCACCAT TCAAGAAAAT 
GAAGTTTCTG AGGATGGAGT CTCGAGTACT GTGGACCAAC TTAGTGACAT TCATATCGAG 
ACGGGAGCCA ATGATTCTCA GCACTCTAAA TGTGATGTAG ATAAGTCTGT GCAACCAGAG 
CCATTTTTCC ATAAAGTGGT TCATTCTGAA CACTTGAACT TAGTCCCTCA AGTTCAATCA 
GTTCAGTGTT CACCAGAAGA ATCCTTTGCA TTTCGATCTC ACTCGCATTT ACCACCAAAA 
AATAAAAACA AGAATTCCTT GCTGATTGGA CTTTCAACTG GTCTGTTTGA TGCAAACAAC 
CCAAAGATGT TAAGGACATG TTCACTTCCA GATCTCTCAA AGCTGTTCAG AACCCTTATG 
GATGTTCCCA CCGTAGGAGA TGTTCGTCAA GACAATCTTG AAATAGATGA AATTGAAGAT 
GAAAACATTA AAGAAGGACC TTCTGATTCT GAAGACATTG TGTTTGAAGA AACTGACACA 
GATTTACAAG AGCTGCAGGC CTCAATGGAA CAGTTACTTA GGGAACAACC TAGTGAAGAA 
TACAGTGAAG AAGAAGAGTC AGTCTTAAAG AACAGTGATG TGGAGCCAAC TGCAAATGGG 
ACAGATGTGG CGGATGAAGA TGACAATCCC AGCAGTGAAA GTGCCCTGAA CGAAGAATGG 
CACTCAGATA ACAGTGATGG GGAAAATGCT AGTGAATGTG AATACGATAG TGTCTTTAAC 
CATTTAGAGG AACTGAGACT TCATCTGGAG CAGGAAATGG GCTTTGAAAA ATTCTTTGAG 
GTTTACGAGA AAATAAAGGC TATTCATGAA GATGAAGATG AAAATATTGA AATTTGTTCA 
AAAATAGTTC AAAATATTTT GGGAAATGAA CATCAGCATC TTTATGCCAA GATTCTTCAT 
TTAGTCATGG CAGATGGAGC CTACCAAGAA GATAATGATG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014994980.2
CDS	650..4294
Translation	MEKYVRLQKIGEGSFGKAILVKSTEDGRQYVIKEINISRMSSKEREESRREVAVLANMKHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGVLFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYELCTLKHAFEAGSMKNLVLKIISGSFPPVSLHYSYDLRSLVSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTFSKFGSQPIPAKRPASGQNSISVMPAQKITKPAAKYGIPLTYKKYGEKKLYEKKPLQKHKQAHQTPEKRVNIGEERRKMSEEAARKRRLEFIEKEKKQKDQIISLMKAEQMKRQEKERLERINRAREQGWRNVLSAGGGGEVKAPFLGSGGTIAPSSFSSRGQYEHYHAIFDQMQQQRAEDNEAKWKREIYGRGLPERQKGQLAVERAKQVEEFLQRKREAMQNKARAEGHMVYLARLRQIRLQNFNERQQIKAKLRGEKKEANRSEGQEGSEEADMRRKKIESLKAHANARAAVLKEQLERKRKEAYEREKKVWEEHLVAKGVKSSDVSPPLGQHETGGSPSKQQMRSVISVTSALKEVGVDSSLTDIREISEEMQKTNNAISSKREILRRLNENLKAQEDEKGKQHLSDTCEINVLEDAKEHEKEKSVSSDRKKWEAGGQLVIPLDELTLDTSFSTTERHTVGEVIKLDPNGSPRRVWGKSPTDSVLKILGEAELQLQTELLENTTIRSDISPEGEKYKPLITEEKKVQCISHEINPSAIVDSPVETKSPEFTEASPQMSLKLEGNLEEPDDLETEVLQEPSGTNKDESLPCTVTDVWISEEKEAKETQLEDRITIQENEVSEDGVSSTVDQLSDIHIETGANDSQHSKCDVDKSVQPEPFFHKVVHSEHLNLVPQVQSVQCSPEESFAFRSHSHLPPKNKNKNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDVRQDNLEIDEIEDENIKEGPSDSEDIVFEETDTDLQELQASMEQLLREQPSEEYSEEEESVLKNSDVEPTANGTDVADEDDNPSSESALNEEWHSDNSDGENASECEYDSVFNHLEELRLHLEQEMGFEKFFEVYEKIKAIHEDEDENIEICSKIVQNILGNEHQHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_015139494.2
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta NIMA related kinase 1 (NEK1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015139494.2

ORF Insert Sequence:

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG CAGACAGTAT GTTATCAAGG AAATTAACAT CTCAAGAATG 
TCCAGTAAAG AAAGAGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GTTCTCTCTA CATAGTGATG 
GATTACTGTG AGGGAGGGGA TCTGTTTAAG CGAATAAATG CTCAGAAAGG CGTTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTGAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATTAAA TCTCAGAACA TATTTTTAAC TAAAGATGGA 
ACGGTACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AATAATAAAA GTGACATTTG GGCTCTGGGG TGTGTCCTTT ATGAGCTGTG TACACTTAAA 
CATGCTTTTG AAGCTGGCAG TATGAAAAAC TTGGTACTGA AGATAATATC CGGATCTTTT 
CCACCTGTGT CTTTGCATTA TTCCTATGAT CTCCGCAGTT TGGTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC ACCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACGTTT 
TCAAAGTTTG GATCACAGCC TATACCAGCC AAAAGACCAG CTTCAGGACA AAACTCGATT 
TCTGTTATGC CTGCTCAGAA AATTACAAAG CCTGCCGCTA AATATGGAAT ACCTTTAACA 
TATAAGAAAT ATGGAGAGAA AAAATTATAC GAAAAGAAAC CACTGCAAAA ACATAAACAG 
GCCCATCAAA CTCCAGAGAA GAGAGTGAAT ATTGGAGAAG AAAGGAGGAA AATGTCTGAG 
GAAGCAGCAA GAAAGAGAAG GCTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTATTAGTT TAATGAAGGC TGAACAAATG AAAAGGCAAG AAAAGGAAAG GTTGGAAAGA 
ATAAATAGGG CCAGGGAACA AGGATGGAGA AATGTGCTAA GTGCTGGTGG AGGTGGTGAA 
GTAAAGGCTC CTTTTCTTGG CAGTGGAGGG ACTATAGCTC CATCATCTTT TTCTTCTCGA 
GGACAGTATG AACATTACCA TGCCATTTTT GACCAAATGC AACAACAGAG AGCAGAAGAT 
AATGAAGCTA AATGGAAAAG AGAAATATAT GGTCGAGGTC TTCCAGAAAG GCAAAAAGGG 
CAGCTAGCTG TAGAAAGAGC TAAACAAGTA GAAGAGTTCC TGCAGCGAAA ACGGGAAGCT 
ATGCAGAATA AAGCTCGAGC CGAAGGACAT ATGGTTTATC TGGCAAGACT GAGGCAAATA 
AGACTACAGA ATTTCAATGA ACGCCAACAG ATTAAAGCCA AACTTCGTGG TGAAAAGAAA 
GAAGCTAATC GTTCTGAAGG ACAAGAAGGA AGTGAAGAGG CTGACATGAG GCGCAAAAAA 
ATCGAATCCC TGAAGGCCCA TGCAAATGCA CGTGCTGCTG TACTAAAAGA ACAACTAGAA 
CGAAAGAGAA AGGAAGCTTA TGAGAGAGAA AAAAAAGTGT GGGAAGAGCA TTTGGTGGCT 
AAAGGAGTTA AGAGTTCTGA TGTTTCTCCA CCTTTGGGAC AGCATGAAAC AGGTGGCTCT 
CCATCAAAGC AACAGATGAG ATCGGTTATT TCTGTGACTT CAGCTTTGAA AGAAGTTGGC 
GTGGACAGTA GTTTAACTGA TATCCGGGAA ATTTCAGAAG AAATGCAAAA GACCAACAAT 
GCTATTTCAA GTAAGCGAGA AATACTTCGT AGATTAAATG AAAATCTTAA AGCTCAAGAA 
GATGAAAAAG GAAAGCAGCA TCTCTCTGAT ACTTGTGAGA TAAATGTTCT TGAAGATGCC 
AAAGAGCATG AAAAAGAAAA ATCAGTTTCA TCTGATCGCA AGAAGTGGGA GGCAGGAGGT 
CAACTTGTGA TTCCTCTGGA TGAGTTAACA CTAGATACAT CCTTCTCTAC AACTGAAAGA 
CATACAGTGG GAGAAGTTAT TAAATTAGAT CCTAATGGAT CTCCAAGAAG AGTCTGGGGG 
AAAAGTCCGA CAGATTCTGT TCTGAAGATA CTTGGAGAAG CTGAACTACA ACTTCAGACA 
GAACTATTAG AAAATACAAC TATTAGAAGT GATATTTCTC CTGAAGGGGA AAAGTACAAA 
CCCTTAATTA CTGAAGAAAA AAAAGTGCAA TGTATTTCAC ATGAAATAAA CCCATCAGCT 
ATTGTTGATT CTCCTGTTGA GACAAAAAGC CCCGAGTTTA CTGAGGCATC TCCACAGATG 
TCATTGAAAC TGGAAGGAAA TTTAGAAGAA CCTGATGATT TGGAAACAGA AGTTCTACAA 
GAGCCAAGTG GAACAAACAA AGATGAGAGC TTGCCATGCA CTGTTACTGA TGTGTGGATT 
AGTGAGGAAA AAGAAGCAAA GGAAACTCAG TTGGAAGATA GGATCACCAT TCAAGAAAAT 
GAAGTTTCTG AGGATGGAGT CTCGAGTACT GTGGACCAAC TTAGTGACAT TCATATCGAG 
ACGGGAGCCA ATGATTCTCA GCACTCTAAA TGTGATGTAG ATAAGTCTGT GCAACCAGAG 
CCATTTTTCC ATAAAGTGGT TCATTCTGAA CACTTGAACT TAGTCCCTCA AGTTCAATCA 
GTTCAGTGTT CACCAGAAGA ATCCTTTGCA TTTCGATCTC ACTCGCATTT ACCACCAAAA 
AATAAAAACA AGAATTCCTT GCTGATTGGA CTTTCAACTG GTCTGTTTGA TGCAAACAAC 
CCAAAGATGT TAAGGACATG TTCACTTCCA GATCTCTCAA AGCTGTTCAG AACCCTTATG 
GATGTTCCCA CCGTAGGAGA TGTTCGTCAA GACAATCTTG AAATAGATGA AATTGAAGAT 
GAAAACATTA AAGAAGGACC TTCTGATTCT GAAGACATTG TGTTTGAAGA AACTGACACA 
GATTTACAAG AGCTGCAGGC CTCAATGGAA CAGTTACTTA GGGAACAACC TAGTGAAGAA 
TACAGTGAAG AAGAAGAGTC AGTCTTAAAG AACAGTGATG TGGAGCCAAC TGCAAATGGG 
ACAGATGTGG CGGATGAAGA TGACAATCCC AGCAGTGAAA GTGCCCTGAA CGAAGAATGG 
CACTCAGATA ACAGTGATGG GGAAAATGCT AGTGAATGTG AATACGATAG TGTCTTTAAC 
CATTTAGAGG AACTGAGACT TCATCTGGAG CAGGAAATGG GCTTTGAAAA ATTCTTTGAG 
GTTTACGAGA AAATAAAGGC TATTCATGAA GATGAAGATG AAAATATTGA AATTTGTTCA 
AAAATAGTTC AAAATATTTT GGGAAATGAA CATCAGCATC TTTATGCCAA GATTCTTCAT 
TTAGTCATGG CAGATGGAGC CTACCAAGAA GATAATGATG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	NEK1
Entrez Gene ID	694345
Full Name	NIMA related kinase 1
Gene Type	protein-coding
Organism	Macaca mulatta(Rhesus monkey)

Bos taurus (cattle)	NEK1	XP_003586402.2
Rattus norvegicus (Norway rat)	Nek1	NP_001099552.2
Gallus gallus (chicken)	NEK1	XP_420401.3
Homo sapiens (human)	NEK1	NP_001186326.1
	NEK1	XP_001083275.1
Canis lupus familiaris (dog)	NEK1	XP_543184.2
Mus musculus (house mouse)	Nek1	NP_780298.2
Pan troglodytes (chimpanzee)	NEK1	XP_526727.2
Danio rerio (zebrafish)	nek1	XP_005160601.1
Xenopus tropicalis (tropical clawed frog)	nek1	NP_001090680.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

NEK1 cDNA ORF clone, Macaca mulatta(Rhesus monkey)

Gene

Clones

gRNAs

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