NEK1 gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_015464414.1	XP_015319900.1	serine/threonine-protein kinase Nek1 isoform X2
XM_015464415.1	XP_015319901.1	serine/threonine-protein kinase Nek1 isoform X3
XM_010807675.2	XP_010805977.1	serine/threonine-protein kinase Nek1 isoform X1
XM_010807677.2	XP_010805979.1	serine/threonine-protein kinase Nek1 isoform X3
XM_010807675.1	XP_010805977.1	serine/threonine-protein kinase Nek1 isoform X1
XM_024996039.1	XP_024851807.1	serine/threonine-protein kinase Nek1 isoform X6
XM_015464413.1	XP_015319899.1	serine/threonine-protein kinase Nek1 isoform X1
XM_024996038.1	XP_024851806.1	serine/threonine-protein kinase Nek1 isoform X4
XM_010807677.1	XP_010805979.1	serine/threonine-protein kinase Nek1 isoform X3
XM_010807676.2	XP_010805978.1	serine/threonine-protein kinase Nek1 isoform X2
XM_015464416.1	XP_015319902.1	serine/threonine-protein kinase Nek1 isoform X4
XM_010807676.1	XP_010805978.1	serine/threonine-protein kinase Nek1 isoform X2
XM_010807678.1	XP_010805980.1	serine/threonine-protein kinase Nek1 isoform X4
XM_010807678.2	XP_010805980.1	serine/threonine-protein kinase Nek1 isoform X5

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa237330	XM_024996038.1 Latest version!	Bos taurus NIMA related kinase 1 (NEK1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa237331	XM_024996039.1 Latest version!	Bos taurus NIMA related kinase 1 (NEK1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa52909	XM_015464413.1 Latest version!	Bos taurus NIMA related kinase 1 (NEK1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa52909	XM_010807675.2 Latest version!	Bos taurus NIMA related kinase 1 (NEK1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa52910	XM_010807676.2 Latest version!	Bos taurus NIMA related kinase 1 (NEK1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa52910	XM_015464414.1 Latest version!	Bos taurus NIMA related kinase 1 (NEK1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa52911	XM_015464415.1 Latest version!	Bos taurus NIMA related kinase 1 (NEK1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa52911	XM_010807677.2 Latest version!	Bos taurus NIMA related kinase 1 (NEK1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa52912	XM_010807678.2 Latest version!	Bos taurus NIMA related kinase 1 (NEK1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa52912	XM_015464416.1 Latest version!	Bos taurus NIMA related kinase 1 (NEK1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
View Old Accession Versions >>
OBa52909	XM_010807675.1	Bos taurus NIMA related kinase 1 (NEK1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa52911	XM_010807677.1	Bos taurus NIMA related kinase 1 (NEK1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa52910	XM_010807676.1	Bos taurus NIMA related kinase 1 (NEK1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa52912	XM_010807678.1	Bos taurus NIMA related kinase 1 (NEK1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
Hide Old Accession Versions >>

NEK1 ( XM_024996038.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024851806.1 Bos taurus NIMA related kinase 1 (NEK1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa237330
Clone ID Related Accession (Same CDS sequence)	XM_024996038.1
Accession Version	XM_024996038.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3576bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	serine/threonine-protein kinase Nek1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAAT ACATTAGAGT ACAGAAGATT GGAGAAGGTT CATTCGGAAA AGCCATTCTT 
GTCAGATCTA CAGAAGATGG CAGACAATAT GTCATCAAAG AAATTAATAT CTCAAGGATG 
TCCAATAAAG AGAGGGAAGA ATCAAGGAGA GAAGTTGCAG TGCTAGCAAA CATGCGGCAT 
CCAAACATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATTGTAATG 
GATTACTGTG AAGGAGGGGA TCTGTTCAAA AGAATAAATG CTCAGAAGGG CGTTTTGTTT 
CAAGAGGACC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTAAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATAAAA TCACAGAACA TATTTTTAAC CAGAGATGGA 
ACAATACAAC TTGGAGATTT TGGAATCGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA TAAACCTTAT 
AATAATAAAA GTGATATTTG GGCTCTGGGG TGTGTCCTTT ATGAGATGTG CACACTTAAA 
CATGCATTTG AAGCTGGCAA TATGAAGAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCCGTGT CTTTGCATTA TTCTTACGAT CTCCGCAGTT TACTCTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC CCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCCAAGTTTG GAGCACAACC TCTACCAGCT AAAAGACCAG CTTCAGGACA AAGCTTGGCT 
TCTATTATGT CTGCTCAGAA AATTACAAAG CCTGCTGCTA AATATGGAAT ACCGTTAACA 
AATAAGAAAT ATATAGATAA AAAATTGCAT GAAAGGAAGC CACTCCAGAA ATATAAACAG 
GAAGCACCAA GAAAAAGAAG ATTGGAATTG ACTGAAAAAG AAAAGAAGTC TAAGGATCAG 
GTTAGTCTGC TGAAGGCTGA GCAGGTGAAA AGACAGGAAA AGCAAAGGTT GGAGAGAATA 
AATAGGGCCA GGGAACAAGG CTGGAGAAAT GTGCTGAGTG CTGGTGGAAG CGGTGAAATG 
AAGGCTCCCT TGTTGGGCAG TGGAGGGGCC ATGGCTCCAT CATCTTTTTC CTCTCGAGGA 
CAGTATGAAC ATTATCACGC TATTTTTGAT CAAATGCAGC AACAAAGAGC AGAAGATAAT 
GAAGCTAAAT GGAAAGGAGA AATACATGGC CAGAGGCTTC CAGAAAGGCA GAAGGGGCAG 
CCAGCTATGG AAAGAGCAAA ACAAGTAGAA GAGTTTCTAC AGCGAAGACG GGAAGCAAAG 
CAGAACAAAG CTCGGGCCGA GGGGCATATG GTTTATTTGG CAAGACTAAG ACAAATAAGA 
CTGCAGAATT TCAATGAACG CCAACAGATT AGAGCCAAGC TTCGTGGTGA AAAGAAGGAA 
ACTGACAGCT CTGAAGATCA AGAAGGAAGT GAGGAGGCTG ATGTGAGGCG TAAAAAAGTG 
GAAGCCCTGA AGGCCCAGGC AAGTGCCCGT GCAGCCGTCC TGAAGGAGCA GCTTGAGCGC 
AAGCGGAAGG AGGCATATGA GAGAGAGAAG AAAGTGTGGG AGGAGCATTT GGTGGCCAAG 
GGAATAAAGA ACTCTGATGT GTCTCCGCCG TCAGGGCAGC AGGAAATGGG GGGTTCCCCA 
ACAAAGCAGC CAATGAGACC TGTTATTTCT GTGACTTCGG CTTTGAAGGA AGTGTGTATG 
GACCAAAGTC TAACTGATGC CCAAGAAACC TCAGAAGAAA TGCAGAAGAG TAACAGTGCT 
GTTTCAAGTA AACGAGAAAT ACTACGTAGA TTAAATCAAA ATCTTAAAGT TCAAGAAAAT 
GAAAAGGGAA GGCAGAGTCG TCGTGAAACG GGTACAGTAA CTGTTCATGA AGATGCCAAA 
GAGCTGGAAA AAGAAAAGTC AGTTGTATCG GATCGAAAAA AATGGGAGAC AGGAGGCCAG 
CTTGTGCTTC CTGTGGACGA ACTGGCCCTG GACGCTTCCT TCTCTGTGAC AGAAAAACAT 
ATAGTGGGAG AGGTTATTAA ATTAGATCCT AGTGGATCTC CAAGAAAAGT CTGGGGGAAA 
AGTCCTACAG ATTCTGTTCT GAGGATACTT GGAGAAGCTG AACTACAACT TCAGACAGAA 
CTACTGGAAA ACACATCTAT TAGAAATGAG ATTTCTCCTG AAAGCGAGAA TTTCAAATCC 
TTAATTACTG AAGAAGAAAA AGTAAAATGT ATTCCACTTG AAATGAACCC ACCAGCTACT 
CTTGATTCTT CTGTTGAGAC AAACAGTCCA AACATTAGTG AAGCATCTCC ACAGACAGCA 
TTGAAAGCAG AAGGAGATTT GGAGGAGCCT GATGACTTGG AGGCAGAAGT GCTGCAGGAG 
CCAAGTGGAG CAGACAGAGA TGGTGGTTTG CCCCAACCTG TTCTCGACAT GTGGGTCACT 
GAGAGAGAAG GAGCAGAGGA GGTTGAATTG GAAGATAGGA TCACCATTCA GCAAAATGAA 
CTTTCTGAAG ATAAAGCCCC GAGCAGTGTG GATCACCTTA GTGAGGTTCA TGTAGAACCT 
GGAGTGGATG ATTCTCCACC CTCTAAGCGC GATATAGATA AATCTATGCA ACCAGAACCA 
TTTTTCCATA AAGTGGTTTA TTCTGAGCAT CTGAAAGCAG TTTCTCAGGT TCAGTCAGTT 
CTCTGTTCAC CAGAAGAATC CTTTCCACTT CGATCTCACT CTGATTCACC ACCAAGGAAT 
AAAAAACAGA ATTCCTTGCT GATTGGACTT TCAACGGGTC TATTTGATGC AAACAACCCA 
AAGATGTTGC GGACATGTTC ACTTCCAGAC CTCTCCAAAC TGTTCAGAAC ACTGATGGAT 
GTCCCCACTG TGGGAGATGG ACGTCAGGAC AATCTTGAAA TGGAAGATGA GCATGTTAAG 
GAAGGGCCGT CGGACTCTGA GGACATTGTG TTTGAAGAGG CTGACACAGA CTTACAGGAG 
CTCCAAGCCT CCATGGAGCA GCTGCTCCAG GAGCAGCCAG GGGAGGACTA CAGCGAAGAG 
GAGGAGTCGG GCTTGAAGAC CAGTGATGAC GCCGAGCAGG CCGCCAACGG CACGGACGCG 
GGCGAGGAGG ACGACAACCC CAGCAGCGAG AGCGCCCTCA ATGAGGAGTG GCACTCGGAT 
AACAGTGATG GGGAGATAAC TAGTGAATGT GAATACGATA GTGTCTTTAA TCATTTAGAG 
GAACTGAGGC TTCACCTGGA ACAGGAAATA GGCTTCGAAA AGTTCTTTGA GGTTTATGAA 
AAAATAAAGG CTGTTCATGA AGATGAAGAT GAAAATATTG AGATTTGTTC AACAATAGTT 
CAGAATATTT TGGGAAATGA ACATCAGCAT CTTTATGCCA AGATTCTTCA TTTAGTCATG 
GCAGATGGAG CCTATCAAGA AGATAATGAT GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024851806.1
CDS	1123..4698
Translation	MEKYIRVQKIGEGSFGKAILVRSTEDGRQYVIKEINISRMSNKEREESRREVAVLANMRHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGVLFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTRDGTIQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIISGSFPPVSLHYSYDLRSLLSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTFSKFGAQPLPAKRPASGQSLASIMSAQKITKPAAKYGIPLTNKKYIDKKLHERKPLQKYKQEAPRKRRLELTEKEKKSKDQVSLLKAEQVKRQEKQRLERINRAREQGWRNVLSAGGSGEMKAPLLGSGGAMAPSSFSSRGQYEHYHAIFDQMQQQRAEDNEAKWKGEIHGQRLPERQKGQPAMERAKQVEEFLQRRREAKQNKARAEGHMVYLARLRQIRLQNFNERQQIRAKLRGEKKETDSSEDQEGSEEADVRRKKVEALKAQASARAAVLKEQLERKRKEAYEREKKVWEEHLVAKGIKNSDVSPPSGQQEMGGSPTKQPMRPVISVTSALKEVCMDQSLTDAQETSEEMQKSNSAVSSKREILRRLNQNLKVQENEKGRQSRRETGTVTVHEDAKELEKEKSVVSDRKKWETGGQLVLPVDELALDASFSVTEKHIVGEVIKLDPSGSPRKVWGKSPTDSVLRILGEAELQLQTELLENTSIRNEISPESENFKSLITEEEKVKCIPLEMNPPATLDSSVETNSPNISEASPQTALKAEGDLEEPDDLEAEVLQEPSGADRDGGLPQPVLDMWVTEREGAEEVELEDRITIQQNELSEDKAPSSVDHLSEVHVEPGVDDSPPSKRDIDKSMQPEPFFHKVVYSEHLKAVSQVQSVLCSPEESFPLRSHSDSPPRNKKQNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDGRQDNLEMEDEHVKEGPSDSEDIVFEEADTDLQELQASMEQLLQEQPGEDYSEEEESGLKTSDDAEQAANGTDAGEEDDNPSSESALNEEWHSDNSDGEITSECEYDSVFNHLEELRLHLEQEIGFEKFFEVYEKIKAVHEDEDENIEICSTIVQNILGNEHQHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_024996038.1
Organism	Bos taurus(cattle)
Definition	Bos taurus NIMA related kinase 1 (NEK1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024996038.1

ORF Insert Sequence:

ATGGAGAAAT ACATTAGAGT ACAGAAGATT GGAGAAGGTT CATTCGGAAA AGCCATTCTT 
GTCAGATCTA CAGAAGATGG CAGACAATAT GTCATCAAAG AAATTAATAT CTCAAGGATG 
TCCAATAAAG AGAGGGAAGA ATCAAGGAGA GAAGTTGCAG TGCTAGCAAA CATGCGGCAT 
CCAAACATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATTGTAATG 
GATTACTGTG AAGGAGGGGA TCTGTTCAAA AGAATAAATG CTCAGAAGGG CGTTTTGTTT 
CAAGAGGACC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTAAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATAAAA TCACAGAACA TATTTTTAAC CAGAGATGGA 
ACAATACAAC TTGGAGATTT TGGAATCGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA TAAACCTTAT 
AATAATAAAA GTGATATTTG GGCTCTGGGG TGTGTCCTTT ATGAGATGTG CACACTTAAA 
CATGCATTTG AAGCTGGCAA TATGAAGAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCCGTGT CTTTGCATTA TTCTTACGAT CTCCGCAGTT TACTCTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC CCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCCAAGTTTG GAGCACAACC TCTACCAGCT AAAAGACCAG CTTCAGGACA AAGCTTGGCT 
TCTATTATGT CTGCTCAGAA AATTACAAAG CCTGCTGCTA AATATGGAAT ACCGTTAACA 
AATAAGAAAT ATATAGATAA AAAATTGCAT GAAAGGAAGC CACTCCAGAA ATATAAACAG 
GAAGCACCAA GAAAAAGAAG ATTGGAATTG ACTGAAAAAG AAAAGAAGTC TAAGGATCAG 
GTTAGTCTGC TGAAGGCTGA GCAGGTGAAA AGACAGGAAA AGCAAAGGTT GGAGAGAATA 
AATAGGGCCA GGGAACAAGG CTGGAGAAAT GTGCTGAGTG CTGGTGGAAG CGGTGAAATG 
AAGGCTCCCT TGTTGGGCAG TGGAGGGGCC ATGGCTCCAT CATCTTTTTC CTCTCGAGGA 
CAGTATGAAC ATTATCACGC TATTTTTGAT CAAATGCAGC AACAAAGAGC AGAAGATAAT 
GAAGCTAAAT GGAAAGGAGA AATACATGGC CAGAGGCTTC CAGAAAGGCA GAAGGGGCAG 
CCAGCTATGG AAAGAGCAAA ACAAGTAGAA GAGTTTCTAC AGCGAAGACG GGAAGCAAAG 
CAGAACAAAG CTCGGGCCGA GGGGCATATG GTTTATTTGG CAAGACTAAG ACAAATAAGA 
CTGCAGAATT TCAATGAACG CCAACAGATT AGAGCCAAGC TTCGTGGTGA AAAGAAGGAA 
ACTGACAGCT CTGAAGATCA AGAAGGAAGT GAGGAGGCTG ATGTGAGGCG TAAAAAAGTG 
GAAGCCCTGA AGGCCCAGGC AAGTGCCCGT GCAGCCGTCC TGAAGGAGCA GCTTGAGCGC 
AAGCGGAAGG AGGCATATGA GAGAGAGAAG AAAGTGTGGG AGGAGCATTT GGTGGCCAAG 
GGAATAAAGA ACTCTGATGT GTCTCCGCCG TCAGGGCAGC AGGAAATGGG GGGTTCCCCA 
ACAAAGCAGC CAATGAGACC TGTTATTTCT GTGACTTCGG CTTTGAAGGA AGTGTGTATG 
GACCAAAGTC TAACTGATGC CCAAGAAACC TCAGAAGAAA TGCAGAAGAG TAACAGTGCT 
GTTTCAAGTA AACGAGAAAT ACTACGTAGA TTAAATCAAA ATCTTAAAGT TCAAGAAAAT 
GAAAAGGGAA GGCAGAGTCG TCGTGAAACG GGTACAGTAA CTGTTCATGA AGATGCCAAA 
GAGCTGGAAA AAGAAAAGTC AGTTGTATCG GATCGAAAAA AATGGGAGAC AGGAGGCCAG 
CTTGTGCTTC CTGTGGACGA ACTGGCCCTG GACGCTTCCT TCTCTGTGAC AGAAAAACAT 
ATAGTGGGAG AGGTTATTAA ATTAGATCCT AGTGGATCTC CAAGAAAAGT CTGGGGGAAA 
AGTCCTACAG ATTCTGTTCT GAGGATACTT GGAGAAGCTG AACTACAACT TCAGACAGAA 
CTACTGGAAA ACACATCTAT TAGAAATGAG ATTTCTCCTG AAAGCGAGAA TTTCAAATCC 
TTAATTACTG AAGAAGAAAA AGTAAAATGT ATTCCACTTG AAATGAACCC ACCAGCTACT 
CTTGATTCTT CTGTTGAGAC AAACAGTCCA AACATTAGTG AAGCATCTCC ACAGACAGCA 
TTGAAAGCAG AAGGAGATTT GGAGGAGCCT GATGACTTGG AGGCAGAAGT GCTGCAGGAG 
CCAAGTGGAG CAGACAGAGA TGGTGGTTTG CCCCAACCTG TTCTCGACAT GTGGGTCACT 
GAGAGAGAAG GAGCAGAGGA GGTTGAATTG GAAGATAGGA TCACCATTCA GCAAAATGAA 
CTTTCTGAAG ATAAAGCCCC GAGCAGTGTG GATCACCTTA GTGAGGTTCA TGTAGAACCT 
GGAGTGGATG ATTCTCCACC CTCTAAGCGC GATATAGATA AATCTATGCA ACCAGAACCA 
TTTTTCCATA AAGTGGTTTA TTCTGAGCAT CTGAAAGCAG TTTCTCAGGT TCAGTCAGTT 
CTCTGTTCAC CAGAAGAATC CTTTCCACTT CGATCTCACT CTGATTCACC ACCAAGGAAT 
AAAAAACAGA ATTCCTTGCT GATTGGACTT TCAACGGGTC TATTTGATGC AAACAACCCA 
AAGATGTTGC GGACATGTTC ACTTCCAGAC CTCTCCAAAC TGTTCAGAAC ACTGATGGAT 
GTCCCCACTG TGGGAGATGG ACGTCAGGAC AATCTTGAAA TGGAAGATGA GCATGTTAAG 
GAAGGGCCGT CGGACTCTGA GGACATTGTG TTTGAAGAGG CTGACACAGA CTTACAGGAG 
CTCCAAGCCT CCATGGAGCA GCTGCTCCAG GAGCAGCCAG GGGAGGACTA CAGCGAAGAG 
GAGGAGTCGG GCTTGAAGAC CAGTGATGAC GCCGAGCAGG CCGCCAACGG CACGGACGCG 
GGCGAGGAGG ACGACAACCC CAGCAGCGAG AGCGCCCTCA ATGAGGAGTG GCACTCGGAT 
AACAGTGATG GGGAGATAAC TAGTGAATGT GAATACGATA GTGTCTTTAA TCATTTAGAG 
GAACTGAGGC TTCACCTGGA ACAGGAAATA GGCTTCGAAA AGTTCTTTGA GGTTTATGAA 
AAAATAAAGG CTGTTCATGA AGATGAAGAT GAAAATATTG AGATTTGTTC AACAATAGTT 
CAGAATATTT TGGGAAATGA ACATCAGCAT CTTTATGCCA AGATTCTTCA TTTAGTCATG 
GCAGATGGAG CCTATCAAGA AGATAATGAT GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_024996039.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024851807.1 Bos taurus NIMA related kinase 1 (NEK1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBa237331
Clone ID Related Accession (Same CDS sequence)	XM_024996039.1
Accession Version	XM_024996039.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3492bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	serine/threonine-protein kinase Nek1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAAT ACATTAGAGT ACAGAAGATT GGAGAAGGTT CATTCGGAAA AGCCATTCTT 
GTCAGATCTA CAGAAGATGG CAGACAATAT GTCATCAAAG AAATTAATAT CTCAAGGATG 
TCCAATAAAG AGAGGGAAGA ATCAAGGAGA GAAGTTGCAG TGCTAGCAAA CATGCGGCAT 
CCAAACATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATTGTAATG 
GATTACTGTG AAGGAGGGGA TCTGTTCAAA AGAATAAATG CTCAGAAGGG CGTTTTGTTT 
CAAGAGGACC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTAAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATAAAA TCACAGAACA TATTTTTAAC CAGAGATGGA 
ACAATACAAC TTGGAGATTT TGGAATCGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA TAAACCTTAT 
AATAATAAAA GTGATATTTG GGCTCTGGGG TGTGTCCTTT ATGAGATGTG CACACTTAAA 
CATGCATTTG AAGCTGGCAA TATGAAGAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCCGTGT CTTTGCATTA TTCTTACGAT CTCCGCAGTT TACTCTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC CCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCCAAGTTTG GAGCACAACC TCTACCAGCT AAAAGACCAG CTTCAGGACA AAGCTTGGCT 
TCTATTATGT CTGCTCAGAA AATTACAAAG CCTGCTGCTA AATATGGAAT ACCGTTAACA 
AATAAGAAAT ATATAGATAA AAAATTGCAT GAAAGGAAGC CACTCCAGAA ATATAAACAG 
GCCCATCAAA CCCCAGTGAA GAAAGTGAAT CCTGGAGAAG AAAGGAGGAA ACTGTTTGAG 
GAAGCACCAA GAAAAAGAAG ATTGGAATTG ACTGAAAAAG AAAAGAAGTC TAAGGATCAG 
GTTAGTCTGC TGAAGGCTGA GCAGGTGAAA AGACAGGAAA AGCAAAGGTT GGAGAGAATA 
AATAGGGCCA GGGAACAAGG CTGGAGAAAT GTGCTGAGTG CTGGTGGAAG CGGTGAAATG 
AAGGCTCCCT TGTTGGGCAG TGGAGGGGCC ATGGCTCCAT CATCTTTTTC CTCTCGAGGA 
CAGTATGAAC ATTATCACGC TATTTTTGAT CAAATGCAGC AACAAAGAGC AGAAGATAAT 
GAAGCTAAAT GGAAAGGAGA AATACATGGC CAGAGGCTTC CAGAAAGGCA GAAGGGGCAG 
CCAGCTATGG AAAGAGCAAA ACAAGTAGAA GAGTTTCTAC AGCGAAGACG GGAAGCAAAG 
CAGAACAAAG CTCGGGCCGA GGGGCATATG GTTTATTTGG CAAGACTAAG ACAAATAAGA 
CTGCAGAATT TCAATGAACG CCAACAGATT AGAGCCAAGC TTCGTGGTGA AAAGAAGGAA 
ACTGACAGCT CTGAAGATCA AGAAGGAAGT GAGGAGGCTG ATGTGAGGCG TAAAAAAGTG 
GAAGCCCTGA AGGCCCAGGC AAGTGCCCGT GCAGCCGTCC TGAAGGAGCA GCTTGAGCGC 
AAGCGGAAGG AGGCATATGA GAGAGAGAAG AAAGTGTGGG AGGAGCATTT GGTGGCCAAG 
GGAATAAAGA ACTCTGATGT GTCTCCGCCG TCAGGGCAGC AGGAAATGGG GGGTTCCCCA 
ACAAAGCAGC CAATGAGACC TGTTATTTCT GTGACTTCGG CTTTGAAGGA AGTGTGTATG 
GACCAAAGTC TAACTGATGC CCAAGAAACC TCAGAAGAAA TGCAGAAGAG TAACAGTGCT 
GTTTCAAGTA AACGAGAAAT ACTACGTAGA TTAAATCAAA ATCTTAAAGT TCAAGAAAAT 
GAAAAGGGAA GGCAGAGTCG TCGTGAAACG GAACATATAG TGGGAGAGGT TATTAAATTA 
GATCCTAGTG GATCTCCAAG AAAAGTCTGG GGGAAAAGTC CTACAGATTC TGTTCTGAGG 
ATACTTGGAG AAGCTGAACT ACAACTTCAG ACAGAACTAC TGGAAAACAC ATCTATTAGA 
AATGAGATTT CTCCTGAAAG CGAGAATTTC AAATCCTTAA TTACTGAAGA AGAAAAAGTA 
AAATGTATTC CACTTGAAAT GAACCCACCA GCTACTCTTG ATTCTTCTGT TGAGACAAAC 
AGTCCAAACA TTAGTGAAGC ATCTCCACAG ACAGCATTGA AAGCAGAAGG AGATTTGGAG 
GAGCCTGATG ACTTGGAGGC AGAAGTGCTG CAGGAGCCAA GTGGAGCAGA CAGAGATGGT 
GGTTTGCCCC AACCTGTTCT CGACATGTGG GTCACTGAGA GAGAAGGAGC AGAGGAGGTT 
GAATTGGAAG ATAGGATCAC CATTCAGCAA AATGAACTTT CTGAAGATAA AGCCCCGAGC 
AGTGTGGATC ACCTTAGTGA GGTTCATGTA GAACCTGGAG TGGATGATTC TCCACCCTCT 
AAGCGCGATA TAGATAAATC TATGCAACCA GAACCATTTT TCCATAAAGT GGTTTATTCT 
GAGCATCTGA AAGCAGTTTC TCAGGTTCAG TCAGTTCTCT GTTCACCAGA AGAATCCTTT 
CCACTTCGAT CTCACTCTGA TTCACCACCA AGGAATAAAA AACAGAATTC CTTGCTGATT 
GGACTTTCAA CGGGTCTATT TGATGCAAAC AACCCAAAGA TGTTGCGGAC ATGTTCACTT 
CCAGACCTCT CCAAACTGTT CAGAACACTG ATGGATGTCC CCACTGTGGG AGATGGACGT 
CAGGACAATC TTGAAATGGA AGATGAGCAT GTTAAGGAAG GGCCGTCGGA CTCTGAGGAC 
ATTGTGTTTG AAGAGGCTGA CACAGACTTA CAGGAGCTCC AAGCCTCCAT GGAGCAGCTG 
CTCCAGGAGC AGCCAGGGGA GGACTACAGC GAAGAGGAGG AGTCGGGCTT GAAGACCAGT 
GATGACGCCG AGCAGGCCGC CAACGGCACG GACGCGGGCG AGGAGGACGA CAACCCCAGC 
AGCGAGAGCG CCCTCAATGA GGAGTGGCAC TCGGATAACA GTGATGGGGA GATAACTAGT 
GAATGTGAAT ACGATAGTGT CTTTAATCAT TTAGAGGAAC TGAGGCTTCA CCTGGAACAG 
GAAATAGGCT TCGAAAAGTT CTTTGAGGTT TATGAAAAAA TAAAGGCTGT TCATGAAGAT 
GAAGATGAAA ATATTGAGAT TTGTTCAACA ATAGTTCAGA ATATTTTGGG AAATGAACAT 
CAGCATCTTT ATGCCAAGAT TCTTCATTTA GTCATGGCAG ATGGAGCCTA TCAAGAAGAT 
AATGATGAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024851807.1
CDS	1123..4614
Translation	MEKYIRVQKIGEGSFGKAILVRSTEDGRQYVIKEINISRMSNKEREESRREVAVLANMRHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGVLFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTRDGTIQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIISGSFPPVSLHYSYDLRSLLSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTFSKFGAQPLPAKRPASGQSLASIMSAQKITKPAAKYGIPLTNKKYIDKKLHERKPLQKYKQAHQTPVKKVNPGEERRKLFEEAPRKRRLELTEKEKKSKDQVSLLKAEQVKRQEKQRLERINRAREQGWRNVLSAGGSGEMKAPLLGSGGAMAPSSFSSRGQYEHYHAIFDQMQQQRAEDNEAKWKGEIHGQRLPERQKGQPAMERAKQVEEFLQRRREAKQNKARAEGHMVYLARLRQIRLQNFNERQQIRAKLRGEKKETDSSEDQEGSEEADVRRKKVEALKAQASARAAVLKEQLERKRKEAYEREKKVWEEHLVAKGIKNSDVSPPSGQQEMGGSPTKQPMRPVISVTSALKEVCMDQSLTDAQETSEEMQKSNSAVSSKREILRRLNQNLKVQENEKGRQSRRETEHIVGEVIKLDPSGSPRKVWGKSPTDSVLRILGEAELQLQTELLENTSIRNEISPESENFKSLITEEEKVKCIPLEMNPPATLDSSVETNSPNISEASPQTALKAEGDLEEPDDLEAEVLQEPSGADRDGGLPQPVLDMWVTEREGAEEVELEDRITIQQNELSEDKAPSSVDHLSEVHVEPGVDDSPPSKRDIDKSMQPEPFFHKVVYSEHLKAVSQVQSVLCSPEESFPLRSHSDSPPRNKKQNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDGRQDNLEMEDEHVKEGPSDSEDIVFEEADTDLQELQASMEQLLQEQPGEDYSEEEESGLKTSDDAEQAANGTDAGEEDDNPSSESALNEEWHSDNSDGEITSECEYDSVFNHLEELRLHLEQEIGFEKFFEVYEKIKAVHEDEDENIEICSTIVQNILGNEHQHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_024996039.1
Organism	Bos taurus(cattle)
Definition	Bos taurus NIMA related kinase 1 (NEK1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024996039.1

ORF Insert Sequence:

ATGGAGAAAT ACATTAGAGT ACAGAAGATT GGAGAAGGTT CATTCGGAAA AGCCATTCTT 
GTCAGATCTA CAGAAGATGG CAGACAATAT GTCATCAAAG AAATTAATAT CTCAAGGATG 
TCCAATAAAG AGAGGGAAGA ATCAAGGAGA GAAGTTGCAG TGCTAGCAAA CATGCGGCAT 
CCAAACATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATTGTAATG 
GATTACTGTG AAGGAGGGGA TCTGTTCAAA AGAATAAATG CTCAGAAGGG CGTTTTGTTT 
CAAGAGGACC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTAAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATAAAA TCACAGAACA TATTTTTAAC CAGAGATGGA 
ACAATACAAC TTGGAGATTT TGGAATCGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA TAAACCTTAT 
AATAATAAAA GTGATATTTG GGCTCTGGGG TGTGTCCTTT ATGAGATGTG CACACTTAAA 
CATGCATTTG AAGCTGGCAA TATGAAGAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCCGTGT CTTTGCATTA TTCTTACGAT CTCCGCAGTT TACTCTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC CCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCCAAGTTTG GAGCACAACC TCTACCAGCT AAAAGACCAG CTTCAGGACA AAGCTTGGCT 
TCTATTATGT CTGCTCAGAA AATTACAAAG CCTGCTGCTA AATATGGAAT ACCGTTAACA 
AATAAGAAAT ATATAGATAA AAAATTGCAT GAAAGGAAGC CACTCCAGAA ATATAAACAG 
GCCCATCAAA CCCCAGTGAA GAAAGTGAAT CCTGGAGAAG AAAGGAGGAA ACTGTTTGAG 
GAAGCACCAA GAAAAAGAAG ATTGGAATTG ACTGAAAAAG AAAAGAAGTC TAAGGATCAG 
GTTAGTCTGC TGAAGGCTGA GCAGGTGAAA AGACAGGAAA AGCAAAGGTT GGAGAGAATA 
AATAGGGCCA GGGAACAAGG CTGGAGAAAT GTGCTGAGTG CTGGTGGAAG CGGTGAAATG 
AAGGCTCCCT TGTTGGGCAG TGGAGGGGCC ATGGCTCCAT CATCTTTTTC CTCTCGAGGA 
CAGTATGAAC ATTATCACGC TATTTTTGAT CAAATGCAGC AACAAAGAGC AGAAGATAAT 
GAAGCTAAAT GGAAAGGAGA AATACATGGC CAGAGGCTTC CAGAAAGGCA GAAGGGGCAG 
CCAGCTATGG AAAGAGCAAA ACAAGTAGAA GAGTTTCTAC AGCGAAGACG GGAAGCAAAG 
CAGAACAAAG CTCGGGCCGA GGGGCATATG GTTTATTTGG CAAGACTAAG ACAAATAAGA 
CTGCAGAATT TCAATGAACG CCAACAGATT AGAGCCAAGC TTCGTGGTGA AAAGAAGGAA 
ACTGACAGCT CTGAAGATCA AGAAGGAAGT GAGGAGGCTG ATGTGAGGCG TAAAAAAGTG 
GAAGCCCTGA AGGCCCAGGC AAGTGCCCGT GCAGCCGTCC TGAAGGAGCA GCTTGAGCGC 
AAGCGGAAGG AGGCATATGA GAGAGAGAAG AAAGTGTGGG AGGAGCATTT GGTGGCCAAG 
GGAATAAAGA ACTCTGATGT GTCTCCGCCG TCAGGGCAGC AGGAAATGGG GGGTTCCCCA 
ACAAAGCAGC CAATGAGACC TGTTATTTCT GTGACTTCGG CTTTGAAGGA AGTGTGTATG 
GACCAAAGTC TAACTGATGC CCAAGAAACC TCAGAAGAAA TGCAGAAGAG TAACAGTGCT 
GTTTCAAGTA AACGAGAAAT ACTACGTAGA TTAAATCAAA ATCTTAAAGT TCAAGAAAAT 
GAAAAGGGAA GGCAGAGTCG TCGTGAAACG GAACATATAG TGGGAGAGGT TATTAAATTA 
GATCCTAGTG GATCTCCAAG AAAAGTCTGG GGGAAAAGTC CTACAGATTC TGTTCTGAGG 
ATACTTGGAG AAGCTGAACT ACAACTTCAG ACAGAACTAC TGGAAAACAC ATCTATTAGA 
AATGAGATTT CTCCTGAAAG CGAGAATTTC AAATCCTTAA TTACTGAAGA AGAAAAAGTA 
AAATGTATTC CACTTGAAAT GAACCCACCA GCTACTCTTG ATTCTTCTGT TGAGACAAAC 
AGTCCAAACA TTAGTGAAGC ATCTCCACAG ACAGCATTGA AAGCAGAAGG AGATTTGGAG 
GAGCCTGATG ACTTGGAGGC AGAAGTGCTG CAGGAGCCAA GTGGAGCAGA CAGAGATGGT 
GGTTTGCCCC AACCTGTTCT CGACATGTGG GTCACTGAGA GAGAAGGAGC AGAGGAGGTT 
GAATTGGAAG ATAGGATCAC CATTCAGCAA AATGAACTTT CTGAAGATAA AGCCCCGAGC 
AGTGTGGATC ACCTTAGTGA GGTTCATGTA GAACCTGGAG TGGATGATTC TCCACCCTCT 
AAGCGCGATA TAGATAAATC TATGCAACCA GAACCATTTT TCCATAAAGT GGTTTATTCT 
GAGCATCTGA AAGCAGTTTC TCAGGTTCAG TCAGTTCTCT GTTCACCAGA AGAATCCTTT 
CCACTTCGAT CTCACTCTGA TTCACCACCA AGGAATAAAA AACAGAATTC CTTGCTGATT 
GGACTTTCAA CGGGTCTATT TGATGCAAAC AACCCAAAGA TGTTGCGGAC ATGTTCACTT 
CCAGACCTCT CCAAACTGTT CAGAACACTG ATGGATGTCC CCACTGTGGG AGATGGACGT 
CAGGACAATC TTGAAATGGA AGATGAGCAT GTTAAGGAAG GGCCGTCGGA CTCTGAGGAC 
ATTGTGTTTG AAGAGGCTGA CACAGACTTA CAGGAGCTCC AAGCCTCCAT GGAGCAGCTG 
CTCCAGGAGC AGCCAGGGGA GGACTACAGC GAAGAGGAGG AGTCGGGCTT GAAGACCAGT 
GATGACGCCG AGCAGGCCGC CAACGGCACG GACGCGGGCG AGGAGGACGA CAACCCCAGC 
AGCGAGAGCG CCCTCAATGA GGAGTGGCAC TCGGATAACA GTGATGGGGA GATAACTAGT 
GAATGTGAAT ACGATAGTGT CTTTAATCAT TTAGAGGAAC TGAGGCTTCA CCTGGAACAG 
GAAATAGGCT TCGAAAAGTT CTTTGAGGTT TATGAAAAAA TAAAGGCTGT TCATGAAGAT 
GAAGATGAAA ATATTGAGAT TTGTTCAACA ATAGTTCAGA ATATTTTGGG AAATGAACAT 
CAGCATCTTT ATGCCAAGAT TCTTCATTTA GTCATGGCAG ATGGAGCCTA TCAAGAAGAT 
AATGATGAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_010807675.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010805977.1 Bos taurus NIMA related kinase 1 (NEK1), transcript variant X1, mRNA.

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CloneID	OBa52909
Clone ID Related Accession (Same CDS sequence)	XM_010807675.2 , XM_010807675.1 , XM_015464413.1
Accession Version	XM_010807675.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3720bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	serine/threonine-protein kinase Nek1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010807675.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAAT ACATTAGAGT ACAGAAGATT GGAGAAGGTT CATTCGGAAA AGCCATTCTT 
GTCAGATCTA CAGAAGATGG CAGACAATAT GTCATCAAAG AAATTAATAT CTCAAGGATG 
TCCAATAAAG AGAGGGAAGA ATCAAGGAGA GAAGTTGCAG TGCTAGCAAA CATGCGGCAT 
CCAAACATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATTGTAATG 
GATTACTGTG AAGGAGGGGA TCTGTTCAAA AGAATAAATG CTCAGAAGGG CGTTTTGTTT 
CAAGAGGACC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTAAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATAAAA TCACAGAACA TATTTTTAAC CAGAGATGGA 
ACAATACAAC TTGGAGATTT TGGAATCGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA TAAACCTTAT 
AATAATAAAA GTGATATTTG GGCTCTGGGG TGTGTCCTTT ATGAGATGTG CACACTTAAA 
CATGCATTTG AAGCTGGCAA TATGAAGAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCCGTGT CTTTGCATTA TTCTTACGAT CTCCGCAGTT TACTCTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC CCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCCAAGTTTG GAGCACAACC TCTACCAGCT AAAAGACCAG CTTCAGGACA AAGCTTGGCT 
TCTATTATGT CTGCTCAGAA AATTACAAAG CCTGCTGCTA AATATGGAAT ACCGTTAACA 
AATAAGAAAT ATATAGATAA AAAATTGCAT GAAAGGAAGC CACTCCAGAA ATATAAACAG 
GCCCATCAAA CCCCAGTGAA GAAAGTGAAT CCTGGAGAAG AAAGGAGGAA ACTGTTTGAG 
GAAGCACCAA GAAAAAGAAG ATTGGAATTG ACTGAAAAAG AAAAGAAGTC TAAGGATCAG 
GTTAGTCTGC TGAAGGCTGA GCAGGTGAAA AGACAGGAAA AGCAAAGGTT GGAGAGAATA 
AATAGGGCCA GGGAACAAGG CTGGAGAAAT GTGCTGAGTG CTGGTGGAAG CGGTGAAATG 
AAGGCTCCCT TGTTGGGCAG TGGAGGGGCC ATGGCTCCAT CATCTTTTTC CTCTCGAGGA 
CAGTATGAAC ATTATCACGC TATTTTTGAT CAAATGCAGC AACAAAGAGC AGAAGATAAT 
GAAGCTAAAT GGAAAGGAGA AATACATGGC CAGAGGCTTC CAGAAAGGCA GAAGGGGCAG 
CCAGCTATGG AAAGAGCAAA ACAAGTAGAA GAGTTTCTAC AGCGAAGACG GGAAGCAAAG 
CAGAACAAAG CTCGGGCCGA GGGGCATATG GGCATCCTGC ACAACCTGGC AGCTCTCTAT 
GGAGGCAGGC CCAGCTCTTC ACGAGGAGGG AAGCCGAGAA ACAAAGAGGA AGAGGTTTAT 
TTGGCAAGAC TAAGACAAAT AAGACTGCAG AATTTCAATG AACGCCAACA GATTAGAGCC 
AAGCTTCGTG GTGAAAAGAA GGAAACTGAC AGCTCTGAAG ATCAAGAAGG AAGTGAGGAG 
GCTGATGTGA GGCGTAAAAA AGTGGAAGCC CTGAAGGCCC AGGCAAGTGC CCGTGCAGCC 
GTCCTGAAGG AGCAGCTTGA GCGCAAGCGG AAGGAGGCAT ATGAGAGAGA GAAGAAAGTG 
TGGGAGGAGC ATTTGGTGGC CAAGGGAATA AAGAACTCTG ATGTGTCTCC GCCGTCAGGG 
CAGCAGGAAA TGGGGGGTTC CCCAACAAAG CAGCCAATGA GACCTGTTAT TTCTGTGACT 
TCGGCTTTGA AGGAAGTGTG TATGGACCAA AGTCTAACTG ATGCCCAAGA AACCTCAGAA 
GAAATGCAGA AGAGTAACAG TGCTGTTTCA AGTAAACGAG AAATACTACG TAGATTAAAT 
CAAAATCTTA AAGTTCAAGA AAATGAAAAG GGAAGGCAGA GTCGTCGTGA AACGGGTACA 
GTAACTGTTC ATGAAGATGC CAAAGAGCTG GAAAAAGAAA AGTCAGTTGT ATCGGATCGA 
AAAAAATGGG AGACAGGAGG CCAGCTTGTG CTTCCTGTGG ACGAACTGGC CCTGGACGCT 
TCCTTCTCTG TGACAGAAAA ACATATAGTG GGAGAGGTTA TTAAATTAGA TCCTAGTGGA 
TCTCCAAGAA AAGTCTGGGG GAAAAGTCCT ACAGATTCTG TTCTGAGGAT ACTTGGAGAA 
GCTGAACTAC AACTTCAGAC AGAACTACTG GAAAACACAT CTATTAGAAA TGAGATTTCT 
CCTGAAAGCG AGAATTTCAA ATCCTTAATT ACTGAAGAAG AAAAAGTAAA ATGTATTCCA 
CTTGAAATGA ACCCACCAGC TACTCTTGAT TCTTCTGTTG AGACAAACAG TCCAAACATT 
AGTGAAGCAT CTCCACAGAC AGCATTGAAA GCAGAAGGAG ATTTGGAGGA GCCTGATGAC 
TTGGAGGCAG AAGTGCTGCA GGAGCCAAGT GGAGCAGACA GAGATGGTGG TTTGCCCCAA 
CCTGTTCTCG ACATGTGGGT CACTGAGAGA GAAGGAGCAG AGGAGGTTGA ATTGGAAGAT 
AGGATCACCA TTCAGCAAAA TGAACTTTCT GAAGATAAAG CCCCGAGCAG TGTGGATCAC 
CTTAGTGAGG TTCATGTAGA ACCTGGAGTG GATGATTCTC CACCCTCTAA GCGCGATATA 
GATAAATCTA TGCAACCAGA ACCATTTTTC CATAAAGTGG TTTATTCTGA GCATCTGAAA 
GCAGTTTCTC AGGTTCAGTC AGTTCTCTGT TCACCAGAAG AATCCTTTCC ACTTCGATCT 
CACTCTGATT CACCACCAAG GAATAAAAAA CAGAATTCCT TGCTGATTGG ACTTTCAACG 
GGTCTATTTG ATGCAAACAA CCCAAAGATG TTGCGGACAT GTTCACTTCC AGACCTCTCC 
AAACTGTTCA GAACACTGAT GGATGTCCCC ACTGTGGGAG ATGGACGTCA GGACAATCTT 
GAAATGGAAG ATGAGCATGT TAAGGAAGGG CCGTCGGACT CTGAGGACAT TGTGTTTGAA 
GAGGCTGACA CAGACTTACA GGAGCTCCAA GCCTCCATGG AGCAGCTGCT CCAGGAGCAG 
CCAGGGGAGG ACTACAGCGA AGAGGAGGAG TCGGGCTTGA AGACCAGTGA TGACGCCGAG 
CAGGCCGCCA ACGGCACGGA CGCGGGCGAG GAGGACGACA ACCCCAGCAG CGAGAGCGCC 
CTCAATGAGG AGTGGCACTC GGATAACAGT GATGGGGAGA TAACTAGTGA ATGTGAATAC 
GATAGTGTCT TTAATCATTT AGAGGAACTG AGGCTTCACC TGGAACAGGA AATAGGCTTC 
GAAAAGTTCT TTGAGGTTTA TGAAAAAATA AAGGCTGTTC ATGAAGATGA AGATGAAAAT 
ATTGAGATTT GTTCAACAAT AGTTCAGAAT ATTTTGGGAA ATGAACATCA GCATCTTTAT 
GCCAAGATTC TTCATTTAGT CATGGCAGAT GGAGCCTATC AAGAAGATAA TGATGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010805977.1
CDS	1123..4842
Translation	MEKYIRVQKIGEGSFGKAILVRSTEDGRQYVIKEINISRMSNKEREESRREVAVLANMRHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGVLFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTRDGTIQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIISGSFPPVSLHYSYDLRSLLSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTFSKFGAQPLPAKRPASGQSLASIMSAQKITKPAAKYGIPLTNKKYIDKKLHERKPLQKYKQAHQTPVKKVNPGEERRKLFEEAPRKRRLELTEKEKKSKDQVSLLKAEQVKRQEKQRLERINRAREQGWRNVLSAGGSGEMKAPLLGSGGAMAPSSFSSRGQYEHYHAIFDQMQQQRAEDNEAKWKGEIHGQRLPERQKGQPAMERAKQVEEFLQRRREAKQNKARAEGHMGILHNLAALYGGRPSSSRGGKPRNKEEEVYLARLRQIRLQNFNERQQIRAKLRGEKKETDSSEDQEGSEEADVRRKKVEALKAQASARAAVLKEQLERKRKEAYEREKKVWEEHLVAKGIKNSDVSPPSGQQEMGGSPTKQPMRPVISVTSALKEVCMDQSLTDAQETSEEMQKSNSAVSSKREILRRLNQNLKVQENEKGRQSRRETGTVTVHEDAKELEKEKSVVSDRKKWETGGQLVLPVDELALDASFSVTEKHIVGEVIKLDPSGSPRKVWGKSPTDSVLRILGEAELQLQTELLENTSIRNEISPESENFKSLITEEEKVKCIPLEMNPPATLDSSVETNSPNISEASPQTALKAEGDLEEPDDLEAEVLQEPSGADRDGGLPQPVLDMWVTEREGAEEVELEDRITIQQNELSEDKAPSSVDHLSEVHVEPGVDDSPPSKRDIDKSMQPEPFFHKVVYSEHLKAVSQVQSVLCSPEESFPLRSHSDSPPRNKKQNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDGRQDNLEMEDEHVKEGPSDSEDIVFEEADTDLQELQASMEQLLQEQPGEDYSEEEESGLKTSDDAEQAANGTDAGEEDDNPSSESALNEEWHSDNSDGEITSECEYDSVFNHLEELRLHLEQEIGFEKFFEVYEKIKAVHEDEDENIEICSTIVQNILGNEHQHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_010807675.2
Organism	Bos taurus(cattle)
Definition	Bos taurus NIMA related kinase 1 (NEK1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010807675.2

ORF Insert Sequence:

ATGGAGAAAT ACATTAGAGT ACAGAAGATT GGAGAAGGTT CATTCGGAAA AGCCATTCTT 
GTCAGATCTA CAGAAGATGG CAGACAATAT GTCATCAAAG AAATTAATAT CTCAAGGATG 
TCCAATAAAG AGAGGGAAGA ATCAAGGAGA GAAGTTGCAG TGCTAGCAAA CATGCGGCAT 
CCAAACATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATTGTAATG 
GATTACTGTG AAGGAGGGGA TCTGTTCAAA AGAATAAATG CTCAGAAGGG CGTTTTGTTT 
CAAGAGGACC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTAAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATAAAA TCACAGAACA TATTTTTAAC CAGAGATGGA 
ACAATACAAC TTGGAGATTT TGGAATCGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA TAAACCTTAT 
AATAATAAAA GTGATATTTG GGCTCTGGGG TGTGTCCTTT ATGAGATGTG CACACTTAAA 
CATGCATTTG AAGCTGGCAA TATGAAGAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCCGTGT CTTTGCATTA TTCTTACGAT CTCCGCAGTT TACTCTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC CCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCCAAGTTTG GAGCACAACC TCTACCAGCT AAAAGACCAG CTTCAGGACA AAGCTTGGCT 
TCTATTATGT CTGCTCAGAA AATTACAAAG CCTGCTGCTA AATATGGAAT ACCGTTAACA 
AATAAGAAAT ATATAGATAA AAAATTGCAT GAAAGGAAGC CACTCCAGAA ATATAAACAG 
GCCCATCAAA CCCCAGTGAA GAAAGTGAAT CCTGGAGAAG AAAGGAGGAA ACTGTTTGAG 
GAAGCACCAA GAAAAAGAAG ATTGGAATTG ACTGAAAAAG AAAAGAAGTC TAAGGATCAG 
GTTAGTCTGC TGAAGGCTGA GCAGGTGAAA AGACAGGAAA AGCAAAGGTT GGAGAGAATA 
AATAGGGCCA GGGAACAAGG CTGGAGAAAT GTGCTGAGTG CTGGTGGAAG CGGTGAAATG 
AAGGCTCCCT TGTTGGGCAG TGGAGGGGCC ATGGCTCCAT CATCTTTTTC CTCTCGAGGA 
CAGTATGAAC ATTATCACGC TATTTTTGAT CAAATGCAGC AACAAAGAGC AGAAGATAAT 
GAAGCTAAAT GGAAAGGAGA AATACATGGC CAGAGGCTTC CAGAAAGGCA GAAGGGGCAG 
CCAGCTATGG AAAGAGCAAA ACAAGTAGAA GAGTTTCTAC AGCGAAGACG GGAAGCAAAG 
CAGAACAAAG CTCGGGCCGA GGGGCATATG GGCATCCTGC ACAACCTGGC AGCTCTCTAT 
GGAGGCAGGC CCAGCTCTTC ACGAGGAGGG AAGCCGAGAA ACAAAGAGGA AGAGGTTTAT 
TTGGCAAGAC TAAGACAAAT AAGACTGCAG AATTTCAATG AACGCCAACA GATTAGAGCC 
AAGCTTCGTG GTGAAAAGAA GGAAACTGAC AGCTCTGAAG ATCAAGAAGG AAGTGAGGAG 
GCTGATGTGA GGCGTAAAAA AGTGGAAGCC CTGAAGGCCC AGGCAAGTGC CCGTGCAGCC 
GTCCTGAAGG AGCAGCTTGA GCGCAAGCGG AAGGAGGCAT ATGAGAGAGA GAAGAAAGTG 
TGGGAGGAGC ATTTGGTGGC CAAGGGAATA AAGAACTCTG ATGTGTCTCC GCCGTCAGGG 
CAGCAGGAAA TGGGGGGTTC CCCAACAAAG CAGCCAATGA GACCTGTTAT TTCTGTGACT 
TCGGCTTTGA AGGAAGTGTG TATGGACCAA AGTCTAACTG ATGCCCAAGA AACCTCAGAA 
GAAATGCAGA AGAGTAACAG TGCTGTTTCA AGTAAACGAG AAATACTACG TAGATTAAAT 
CAAAATCTTA AAGTTCAAGA AAATGAAAAG GGAAGGCAGA GTCGTCGTGA AACGGGTACA 
GTAACTGTTC ATGAAGATGC CAAAGAGCTG GAAAAAGAAA AGTCAGTTGT ATCGGATCGA 
AAAAAATGGG AGACAGGAGG CCAGCTTGTG CTTCCTGTGG ACGAACTGGC CCTGGACGCT 
TCCTTCTCTG TGACAGAAAA ACATATAGTG GGAGAGGTTA TTAAATTAGA TCCTAGTGGA 
TCTCCAAGAA AAGTCTGGGG GAAAAGTCCT ACAGATTCTG TTCTGAGGAT ACTTGGAGAA 
GCTGAACTAC AACTTCAGAC AGAACTACTG GAAAACACAT CTATTAGAAA TGAGATTTCT 
CCTGAAAGCG AGAATTTCAA ATCCTTAATT ACTGAAGAAG AAAAAGTAAA ATGTATTCCA 
CTTGAAATGA ACCCACCAGC TACTCTTGAT TCTTCTGTTG AGACAAACAG TCCAAACATT 
AGTGAAGCAT CTCCACAGAC AGCATTGAAA GCAGAAGGAG ATTTGGAGGA GCCTGATGAC 
TTGGAGGCAG AAGTGCTGCA GGAGCCAAGT GGAGCAGACA GAGATGGTGG TTTGCCCCAA 
CCTGTTCTCG ACATGTGGGT CACTGAGAGA GAAGGAGCAG AGGAGGTTGA ATTGGAAGAT 
AGGATCACCA TTCAGCAAAA TGAACTTTCT GAAGATAAAG CCCCGAGCAG TGTGGATCAC 
CTTAGTGAGG TTCATGTAGA ACCTGGAGTG GATGATTCTC CACCCTCTAA GCGCGATATA 
GATAAATCTA TGCAACCAGA ACCATTTTTC CATAAAGTGG TTTATTCTGA GCATCTGAAA 
GCAGTTTCTC AGGTTCAGTC AGTTCTCTGT TCACCAGAAG AATCCTTTCC ACTTCGATCT 
CACTCTGATT CACCACCAAG GAATAAAAAA CAGAATTCCT TGCTGATTGG ACTTTCAACG 
GGTCTATTTG ATGCAAACAA CCCAAAGATG TTGCGGACAT GTTCACTTCC AGACCTCTCC 
AAACTGTTCA GAACACTGAT GGATGTCCCC ACTGTGGGAG ATGGACGTCA GGACAATCTT 
GAAATGGAAG ATGAGCATGT TAAGGAAGGG CCGTCGGACT CTGAGGACAT TGTGTTTGAA 
GAGGCTGACA CAGACTTACA GGAGCTCCAA GCCTCCATGG AGCAGCTGCT CCAGGAGCAG 
CCAGGGGAGG ACTACAGCGA AGAGGAGGAG TCGGGCTTGA AGACCAGTGA TGACGCCGAG 
CAGGCCGCCA ACGGCACGGA CGCGGGCGAG GAGGACGACA ACCCCAGCAG CGAGAGCGCC 
CTCAATGAGG AGTGGCACTC GGATAACAGT GATGGGGAGA TAACTAGTGA ATGTGAATAC 
GATAGTGTCT TTAATCATTT AGAGGAACTG AGGCTTCACC TGGAACAGGA AATAGGCTTC 
GAAAAGTTCT TTGAGGTTTA TGAAAAAATA AAGGCTGTTC ATGAAGATGA AGATGAAAAT 
ATTGAGATTT GTTCAACAAT AGTTCAGAAT ATTTTGGGAA ATGAACATCA GCATCTTTAT 
GCCAAGATTC TTCATTTAGT CATGGCAGAT GGAGCCTATC AAGAAGATAA TGATGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_010807675.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010805977.1 Bos taurus NIMA related kinase 1 (NEK1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa52909
Clone ID Related Accession (Same CDS sequence)	XM_010807675.2 , XM_010807675.1 , XM_015464413.1
Accession Version	XM_010807675.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3720bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	serine/threonine-protein kinase Nek1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104069.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAAT ACATTAGAGT ACAGAAGATT GGAGAAGGTT CATTCGGAAA AGCCATTCTT 
GTCAGATCTA CAGAAGATGG CAGACAATAT GTCATCAAAG AAATTAATAT CTCAAGGATG 
TCCAATAAAG AGAGGGAAGA ATCAAGGAGA GAAGTTGCAG TGCTAGCAAA CATGCGGCAT 
CCAAACATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATTGTAATG 
GATTACTGTG AAGGAGGGGA TCTGTTCAAA AGAATAAATG CTCAGAAGGG CGTTTTGTTT 
CAAGAGGACC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTAAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATAAAA TCACAGAACA TATTTTTAAC CAGAGATGGA 
ACAATACAAC TTGGAGATTT TGGAATCGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA TAAACCTTAT 
AATAATAAAA GTGATATTTG GGCTCTGGGG TGTGTCCTTT ATGAGATGTG CACACTTAAA 
CATGCATTTG AAGCTGGCAA TATGAAGAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCCGTGT CTTTGCATTA TTCTTACGAT CTCCGCAGTT TACTCTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC CCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCCAAGTTTG GAGCACAACC TCTACCAGCT AAAAGACCAG CTTCAGGACA AAGCTTGGCT 
TCTATTATGT CTGCTCAGAA AATTACAAAG CCTGCTGCTA AATATGGAAT ACCGTTAACA 
AATAAGAAAT ATATAGATAA AAAATTGCAT GAAAGGAAGC CACTCCAGAA ATATAAACAG 
GCCCATCAAA CCCCAGTGAA GAAAGTGAAT CCTGGAGAAG AAAGGAGGAA ACTGTTTGAG 
GAAGCACCAA GAAAAAGAAG ATTGGAATTG ACTGAAAAAG AAAAGAAGTC TAAGGATCAG 
GTTAGTCTGC TGAAGGCTGA GCAGGTGAAA AGACAGGAAA AGCAAAGGTT GGAGAGAATA 
AATAGGGCCA GGGAACAAGG CTGGAGAAAT GTGCTGAGTG CTGGTGGAAG CGGTGAAATG 
AAGGCTCCCT TGTTGGGCAG TGGAGGGGCC ATGGCTCCAT CATCTTTTTC CTCTCGAGGA 
CAGTATGAAC ATTATCACGC TATTTTTGAT CAAATGCAGC AACAAAGAGC AGAAGATAAT 
GAAGCTAAAT GGAAAGGAGA AATACATGGC CAGAGGCTTC CAGAAAGGCA GAAGGGGCAG 
CCAGCTATGG AAAGAGCAAA ACAAGTAGAA GAGTTTCTAC AGCGAAGACG GGAAGCAAAG 
CAGAACAAAG CTCGGGCCGA GGGGCATATG GGCATCCTGC ACAACCTGGC AGCTCTCTAT 
GGAGGCAGGC CCAGCTCTTC ACGAGGAGGG AAGCCGAGAA ACAAAGAGGA AGAGGTTTAT 
TTGGCAAGAC TAAGACAAAT AAGACTGCAG AATTTCAATG AACGCCAACA GATTAGAGCC 
AAGCTTCGTG GTGAAAAGAA GGAAACTGAC AGCTCTGAAG ATCAAGAAGG AAGTGAGGAG 
GCTGATGTGA GGCGTAAAAA AGTGGAAGCC CTGAAGGCCC AGGCAAGTGC CCGTGCAGCC 
GTCCTGAAGG AGCAGCTTGA GCGCAAGCGG AAGGAGGCAT ATGAGAGAGA GAAGAAAGTG 
TGGGAGGAGC ATTTGGTGGC CAAGGGAATA AAGAACTCTG ATGTGTCTCC GCCGTCAGGG 
CAGCAGGAAA TGGGGGGTTC CCCAACAAAG CAGCCAATGA GACCTGTTAT TTCTGTGACT 
TCGGCTTTGA AGGAAGTGTG TATGGACCAA AGTCTAACTG ATGCCCAAGA AACCTCAGAA 
GAAATGCAGA AGAGTAACAG TGCTGTTTCA AGTAAACGAG AAATACTACG TAGATTAAAT 
CAAAATCTTA AAGTTCAAGA AAATGAAAAG GGAAGGCAGA GTCGTCGTGA AACGGGTACA 
GTAACTGTTC ATGAAGATGC CAAAGAGCTG GAAAAAGAAA AGTCAGTTGT ATCGGATCGA 
AAAAAATGGG AGACAGGAGG CCAGCTTGTG CTTCCTGTGG ACGAACTGGC CCTGGACGCT 
TCCTTCTCTG TGACAGAAAA ACATATAGTG GGAGAGGTTA TTAAATTAGA TCCTAGTGGA 
TCTCCAAGAA AAGTCTGGGG GAAAAGTCCT ACAGATTCTG TTCTGAGGAT ACTTGGAGAA 
GCTGAACTAC AACTTCAGAC AGAACTACTG GAAAACACAT CTATTAGAAA TGAGATTTCT 
CCTGAAAGCG AGAATTTCAA ATCCTTAATT ACTGAAGAAG AAAAAGTAAA ATGTATTCCA 
CTTGAAATGA ACCCACCAGC TACTCTTGAT TCTTCTGTTG AGACAAACAG TCCAAACATT 
AGTGAAGCAT CTCCACAGAC AGCATTGAAA GCAGAAGGAG ATTTGGAGGA GCCTGATGAC 
TTGGAGGCAG AAGTGCTGCA GGAGCCAAGT GGAGCAGACA GAGATGGTGG TTTGCCCCAA 
CCTGTTCTCG ACATGTGGGT CACTGAGAGA GAAGGAGCAG AGGAGGTTGA ATTGGAAGAT 
AGGATCACCA TTCAGCAAAA TGAACTTTCT GAAGATAAAG CCCCGAGCAG TGTGGATCAC 
CTTAGTGAGG TTCATGTAGA ACCTGGAGTG GATGATTCTC CACCCTCTAA GCGCGATATA 
GATAAATCTA TGCAACCAGA ACCATTTTTC CATAAAGTGG TTTATTCTGA GCATCTGAAA 
GCAGTTTCTC AGGTTCAGTC AGTTCTCTGT TCACCAGAAG AATCCTTTCC ACTTCGATCT 
CACTCTGATT CACCACCAAG GAATAAAAAA CAGAATTCCT TGCTGATTGG ACTTTCAACG 
GGTCTATTTG ATGCAAACAA CCCAAAGATG TTGCGGACAT GTTCACTTCC AGACCTCTCC 
AAACTGTTCA GAACACTGAT GGATGTCCCC ACTGTGGGAG ATGGACGTCA GGACAATCTT 
GAAATGGAAG ATGAGCATGT TAAGGAAGGG CCGTCGGACT CTGAGGACAT TGTGTTTGAA 
GAGGCTGACA CAGACTTACA GGAGCTCCAA GCCTCCATGG AGCAGCTGCT CCAGGAGCAG 
CCAGGGGAGG ACTACAGCGA AGAGGAGGAG TCGGGCTTGA AGACCAGTGA TGACGCCGAG 
CAGGCCGCCA ACGGCACGGA CGCGGGCGAG GAGGACGACA ACCCCAGCAG CGAGAGCGCC 
CTCAATGAGG AGTGGCACTC GGATAACAGT GATGGGGAGA TAACTAGTGA ATGTGAATAC 
GATAGTGTCT TTAATCATTT AGAGGAACTG AGGCTTCACC TGGAACAGGA AATAGGCTTC 
GAAAAGTTCT TTGAGGTTTA TGAAAAAATA AAGGCTGTTC ATGAAGATGA AGATGAAAAT 
ATTGAGATTT GTTCAACAAT AGTTCAGAAT ATTTTGGGAA ATGAACATCA GCATCTTTAT 
GCCAAGATTC TTCATTTAGT CATGGCAGAT GGAGCCTATC AAGAAGATAA TGATGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010805977.1
CDS	770..4489
Translation	MEKYIRVQKIGEGSFGKAILVRSTEDGRQYVIKEINISRMSNKEREESRREVAVLANMRHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGVLFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTRDGTIQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIISGSFPPVSLHYSYDLRSLLSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTFSKFGAQPLPAKRPASGQSLASIMSAQKITKPAAKYGIPLTNKKYIDKKLHERKPLQKYKQAHQTPVKKVNPGEERRKLFEEAPRKRRLELTEKEKKSKDQVSLLKAEQVKRQEKQRLERINRAREQGWRNVLSAGGSGEMKAPLLGSGGAMAPSSFSSRGQYEHYHAIFDQMQQQRAEDNEAKWKGEIHGQRLPERQKGQPAMERAKQVEEFLQRRREAKQNKARAEGHMGILHNLAALYGGRPSSSRGGKPRNKEEEVYLARLRQIRLQNFNERQQIRAKLRGEKKETDSSEDQEGSEEADVRRKKVEALKAQASARAAVLKEQLERKRKEAYEREKKVWEEHLVAKGIKNSDVSPPSGQQEMGGSPTKQPMRPVISVTSALKEVCMDQSLTDAQETSEEMQKSNSAVSSKREILRRLNQNLKVQENEKGRQSRRETGTVTVHEDAKELEKEKSVVSDRKKWETGGQLVLPVDELALDASFSVTEKHIVGEVIKLDPSGSPRKVWGKSPTDSVLRILGEAELQLQTELLENTSIRNEISPESENFKSLITEEEKVKCIPLEMNPPATLDSSVETNSPNISEASPQTALKAEGDLEEPDDLEAEVLQEPSGADRDGGLPQPVLDMWVTEREGAEEVELEDRITIQQNELSEDKAPSSVDHLSEVHVEPGVDDSPPSKRDIDKSMQPEPFFHKVVYSEHLKAVSQVQSVLCSPEESFPLRSHSDSPPRNKKQNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDGRQDNLEMEDEHVKEGPSDSEDIVFEEADTDLQELQASMEQLLQEQPGEDYSEEEESGLKTSDDAEQAANGTDAGEEDDNPSSESALNEEWHSDNSDGEITSECEYDSVFNHLEELRLHLEQEIGFEKFFEVYEKIKAVHEDEDENIEICSTIVQNILGNEHQHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_010807675.1
Organism	Bos taurus(cattle)
Definition	Bos taurus NIMA related kinase 1 (NEK1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010807675.1

ORF Insert Sequence:

ATGGAGAAAT ACATTAGAGT ACAGAAGATT GGAGAAGGTT CATTCGGAAA AGCCATTCTT 
GTCAGATCTA CAGAAGATGG CAGACAATAT GTCATCAAAG AAATTAATAT CTCAAGGATG 
TCCAATAAAG AGAGGGAAGA ATCAAGGAGA GAAGTTGCAG TGCTAGCAAA CATGCGGCAT 
CCAAACATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATTGTAATG 
GATTACTGTG AAGGAGGGGA TCTGTTCAAA AGAATAAATG CTCAGAAGGG CGTTTTGTTT 
CAAGAGGACC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTAAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATAAAA TCACAGAACA TATTTTTAAC CAGAGATGGA 
ACAATACAAC TTGGAGATTT TGGAATCGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA TAAACCTTAT 
AATAATAAAA GTGATATTTG GGCTCTGGGG TGTGTCCTTT ATGAGATGTG CACACTTAAA 
CATGCATTTG AAGCTGGCAA TATGAAGAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCCGTGT CTTTGCATTA TTCTTACGAT CTCCGCAGTT TACTCTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC CCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCCAAGTTTG GAGCACAACC TCTACCAGCT AAAAGACCAG CTTCAGGACA AAGCTTGGCT 
TCTATTATGT CTGCTCAGAA AATTACAAAG CCTGCTGCTA AATATGGAAT ACCGTTAACA 
AATAAGAAAT ATATAGATAA AAAATTGCAT GAAAGGAAGC CACTCCAGAA ATATAAACAG 
GCCCATCAAA CCCCAGTGAA GAAAGTGAAT CCTGGAGAAG AAAGGAGGAA ACTGTTTGAG 
GAAGCACCAA GAAAAAGAAG ATTGGAATTG ACTGAAAAAG AAAAGAAGTC TAAGGATCAG 
GTTAGTCTGC TGAAGGCTGA GCAGGTGAAA AGACAGGAAA AGCAAAGGTT GGAGAGAATA 
AATAGGGCCA GGGAACAAGG CTGGAGAAAT GTGCTGAGTG CTGGTGGAAG CGGTGAAATG 
AAGGCTCCCT TGTTGGGCAG TGGAGGGGCC ATGGCTCCAT CATCTTTTTC CTCTCGAGGA 
CAGTATGAAC ATTATCACGC TATTTTTGAT CAAATGCAGC AACAAAGAGC AGAAGATAAT 
GAAGCTAAAT GGAAAGGAGA AATACATGGC CAGAGGCTTC CAGAAAGGCA GAAGGGGCAG 
CCAGCTATGG AAAGAGCAAA ACAAGTAGAA GAGTTTCTAC AGCGAAGACG GGAAGCAAAG 
CAGAACAAAG CTCGGGCCGA GGGGCATATG GGCATCCTGC ACAACCTGGC AGCTCTCTAT 
GGAGGCAGGC CCAGCTCTTC ACGAGGAGGG AAGCCGAGAA ACAAAGAGGA AGAGGTTTAT 
TTGGCAAGAC TAAGACAAAT AAGACTGCAG AATTTCAATG AACGCCAACA GATTAGAGCC 
AAGCTTCGTG GTGAAAAGAA GGAAACTGAC AGCTCTGAAG ATCAAGAAGG AAGTGAGGAG 
GCTGATGTGA GGCGTAAAAA AGTGGAAGCC CTGAAGGCCC AGGCAAGTGC CCGTGCAGCC 
GTCCTGAAGG AGCAGCTTGA GCGCAAGCGG AAGGAGGCAT ATGAGAGAGA GAAGAAAGTG 
TGGGAGGAGC ATTTGGTGGC CAAGGGAATA AAGAACTCTG ATGTGTCTCC GCCGTCAGGG 
CAGCAGGAAA TGGGGGGTTC CCCAACAAAG CAGCCAATGA GACCTGTTAT TTCTGTGACT 
TCGGCTTTGA AGGAAGTGTG TATGGACCAA AGTCTAACTG ATGCCCAAGA AACCTCAGAA 
GAAATGCAGA AGAGTAACAG TGCTGTTTCA AGTAAACGAG AAATACTACG TAGATTAAAT 
CAAAATCTTA AAGTTCAAGA AAATGAAAAG GGAAGGCAGA GTCGTCGTGA AACGGGTACA 
GTAACTGTTC ATGAAGATGC CAAAGAGCTG GAAAAAGAAA AGTCAGTTGT ATCGGATCGA 
AAAAAATGGG AGACAGGAGG CCAGCTTGTG CTTCCTGTGG ACGAACTGGC CCTGGACGCT 
TCCTTCTCTG TGACAGAAAA ACATATAGTG GGAGAGGTTA TTAAATTAGA TCCTAGTGGA 
TCTCCAAGAA AAGTCTGGGG GAAAAGTCCT ACAGATTCTG TTCTGAGGAT ACTTGGAGAA 
GCTGAACTAC AACTTCAGAC AGAACTACTG GAAAACACAT CTATTAGAAA TGAGATTTCT 
CCTGAAAGCG AGAATTTCAA ATCCTTAATT ACTGAAGAAG AAAAAGTAAA ATGTATTCCA 
CTTGAAATGA ACCCACCAGC TACTCTTGAT TCTTCTGTTG AGACAAACAG TCCAAACATT 
AGTGAAGCAT CTCCACAGAC AGCATTGAAA GCAGAAGGAG ATTTGGAGGA GCCTGATGAC 
TTGGAGGCAG AAGTGCTGCA GGAGCCAAGT GGAGCAGACA GAGATGGTGG TTTGCCCCAA 
CCTGTTCTCG ACATGTGGGT CACTGAGAGA GAAGGAGCAG AGGAGGTTGA ATTGGAAGAT 
AGGATCACCA TTCAGCAAAA TGAACTTTCT GAAGATAAAG CCCCGAGCAG TGTGGATCAC 
CTTAGTGAGG TTCATGTAGA ACCTGGAGTG GATGATTCTC CACCCTCTAA GCGCGATATA 
GATAAATCTA TGCAACCAGA ACCATTTTTC CATAAAGTGG TTTATTCTGA GCATCTGAAA 
GCAGTTTCTC AGGTTCAGTC AGTTCTCTGT TCACCAGAAG AATCCTTTCC ACTTCGATCT 
CACTCTGATT CACCACCAAG GAATAAAAAA CAGAATTCCT TGCTGATTGG ACTTTCAACG 
GGTCTATTTG ATGCAAACAA CCCAAAGATG TTGCGGACAT GTTCACTTCC AGACCTCTCC 
AAACTGTTCA GAACACTGAT GGATGTCCCC ACTGTGGGAG ATGGACGTCA GGACAATCTT 
GAAATGGAAG ATGAGCATGT TAAGGAAGGG CCGTCGGACT CTGAGGACAT TGTGTTTGAA 
GAGGCTGACA CAGACTTACA GGAGCTCCAA GCCTCCATGG AGCAGCTGCT CCAGGAGCAG 
CCAGGGGAGG ACTACAGCGA AGAGGAGGAG TCGGGCTTGA AGACCAGTGA TGACGCCGAG 
CAGGCCGCCA ACGGCACGGA CGCGGGCGAG GAGGACGACA ACCCCAGCAG CGAGAGCGCC 
CTCAATGAGG AGTGGCACTC GGATAACAGT GATGGGGAGA TAACTAGTGA ATGTGAATAC 
GATAGTGTCT TTAATCATTT AGAGGAACTG AGGCTTCACC TGGAACAGGA AATAGGCTTC 
GAAAAGTTCT TTGAGGTTTA TGAAAAAATA AAGGCTGTTC ATGAAGATGA AGATGAAAAT 
ATTGAGATTT GTTCAACAAT AGTTCAGAAT ATTTTGGGAA ATGAACATCA GCATCTTTAT 
GCCAAGATTC TTCATTTAGT CATGGCAGAT GGAGCCTATC AAGAAGATAA TGATGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_015464413.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015319899.1 Bos taurus NIMA related kinase 1 (NEK1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa52909
Clone ID Related Accession (Same CDS sequence)	XM_010807675.2 , XM_010807675.1 , XM_015464413.1
Accession Version	XM_015464413.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3720bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	serine/threonine-protein kinase Nek1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645187.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAAT ACATTAGAGT ACAGAAGATT GGAGAAGGTT CATTCGGAAA AGCCATTCTT 
GTCAGATCTA CAGAAGATGG CAGACAATAT GTCATCAAAG AAATTAATAT CTCAAGGATG 
TCCAATAAAG AGAGGGAAGA ATCAAGGAGA GAAGTTGCAG TGCTAGCAAA CATGCGGCAT 
CCAAACATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATTGTAATG 
GATTACTGTG AAGGAGGGGA TCTGTTCAAA AGAATAAATG CTCAGAAGGG CGTTTTGTTT 
CAAGAGGACC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTAAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATAAAA TCACAGAACA TATTTTTAAC CAGAGATGGA 
ACAATACAAC TTGGAGATTT TGGAATCGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA TAAACCTTAT 
AATAATAAAA GTGATATTTG GGCTCTGGGG TGTGTCCTTT ATGAGATGTG CACACTTAAA 
CATGCATTTG AAGCTGGCAA TATGAAGAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCCGTGT CTTTGCATTA TTCTTACGAT CTCCGCAGTT TACTCTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC CCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCCAAGTTTG GAGCACAACC TCTACCAGCT AAAAGACCAG CTTCAGGACA AAGCTTGGCT 
TCTATTATGT CTGCTCAGAA AATTACAAAG CCTGCTGCTA AATATGGAAT ACCGTTAACA 
AATAAGAAAT ATATAGATAA AAAATTGCAT GAAAGGAAGC CACTCCAGAA ATATAAACAG 
GCCCATCAAA CCCCAGTGAA GAAAGTGAAT CCTGGAGAAG AAAGGAGGAA ACTGTTTGAG 
GAAGCACCAA GAAAAAGAAG ATTGGAATTG ACTGAAAAAG AAAAGAAGTC TAAGGATCAG 
GTTAGTCTGC TGAAGGCTGA GCAGGTGAAA AGACAGGAAA AGCAAAGGTT GGAGAGAATA 
AATAGGGCCA GGGAACAAGG CTGGAGAAAT GTGCTGAGTG CTGGTGGAAG CGGTGAAATG 
AAGGCTCCCT TGTTGGGCAG TGGAGGGGCC ATGGCTCCAT CATCTTTTTC CTCTCGAGGA 
CAGTATGAAC ATTATCACGC TATTTTTGAT CAAATGCAGC AACAAAGAGC AGAAGATAAT 
GAAGCTAAAT GGAAAGGAGA AATACATGGC CAGAGGCTTC CAGAAAGGCA GAAGGGGCAG 
CCAGCTATGG AAAGAGCAAA ACAAGTAGAA GAGTTTCTAC AGCGAAGACG GGAAGCAAAG 
CAGAACAAAG CTCGGGCCGA GGGGCATATG GGCATCCTGC ACAACCTGGC AGCTCTCTAT 
GGAGGCAGGC CCAGCTCTTC ACGAGGAGGG AAGCCGAGAA ACAAAGAGGA AGAGGTTTAT 
TTGGCAAGAC TAAGACAAAT AAGACTGCAG AATTTCAATG AACGCCAACA GATTAGAGCC 
AAGCTTCGTG GTGAAAAGAA GGAAACTGAC AGCTCTGAAG ATCAAGAAGG AAGTGAGGAG 
GCTGATGTGA GGCGTAAAAA AGTGGAAGCC CTGAAGGCCC AGGCAAGTGC CCGTGCAGCC 
GTCCTGAAGG AGCAGCTTGA GCGCAAGCGG AAGGAGGCAT ATGAGAGAGA GAAGAAAGTG 
TGGGAGGAGC ATTTGGTGGC CAAGGGAATA AAGAACTCTG ATGTGTCTCC GCCGTCAGGG 
CAGCAGGAAA TGGGGGGTTC CCCAACAAAG CAGCCAATGA GACCTGTTAT TTCTGTGACT 
TCGGCTTTGA AGGAAGTGTG TATGGACCAA AGTCTAACTG ATGCCCAAGA AACCTCAGAA 
GAAATGCAGA AGAGTAACAG TGCTGTTTCA AGTAAACGAG AAATACTACG TAGATTAAAT 
CAAAATCTTA AAGTTCAAGA AAATGAAAAG GGAAGGCAGA GTCGTCGTGA AACGGGTACA 
GTAACTGTTC ATGAAGATGC CAAAGAGCTG GAAAAAGAAA AGTCAGTTGT ATCGGATCGA 
AAAAAATGGG AGACAGGAGG CCAGCTTGTG CTTCCTGTGG ACGAACTGGC CCTGGACGCT 
TCCTTCTCTG TGACAGAAAA ACATATAGTG GGAGAGGTTA TTAAATTAGA TCCTAGTGGA 
TCTCCAAGAA AAGTCTGGGG GAAAAGTCCT ACAGATTCTG TTCTGAGGAT ACTTGGAGAA 
GCTGAACTAC AACTTCAGAC AGAACTACTG GAAAACACAT CTATTAGAAA TGAGATTTCT 
CCTGAAAGCG AGAATTTCAA ATCCTTAATT ACTGAAGAAG AAAAAGTAAA ATGTATTCCA 
CTTGAAATGA ACCCACCAGC TACTCTTGAT TCTTCTGTTG AGACAAACAG TCCAAACATT 
AGTGAAGCAT CTCCACAGAC AGCATTGAAA GCAGAAGGAG ATTTGGAGGA GCCTGATGAC 
TTGGAGGCAG AAGTGCTGCA GGAGCCAAGT GGAGCAGACA GAGATGGTGG TTTGCCCCAA 
CCTGTTCTCG ACATGTGGGT CACTGAGAGA GAAGGAGCAG AGGAGGTTGA ATTGGAAGAT 
AGGATCACCA TTCAGCAAAA TGAACTTTCT GAAGATAAAG CCCCGAGCAG TGTGGATCAC 
CTTAGTGAGG TTCATGTAGA ACCTGGAGTG GATGATTCTC CACCCTCTAA GCGCGATATA 
GATAAATCTA TGCAACCAGA ACCATTTTTC CATAAAGTGG TTTATTCTGA GCATCTGAAA 
GCAGTTTCTC AGGTTCAGTC AGTTCTCTGT TCACCAGAAG AATCCTTTCC ACTTCGATCT 
CACTCTGATT CACCACCAAG GAATAAAAAA CAGAATTCCT TGCTGATTGG ACTTTCAACG 
GGTCTATTTG ATGCAAACAA CCCAAAGATG TTGCGGACAT GTTCACTTCC AGACCTCTCC 
AAACTGTTCA GAACACTGAT GGATGTCCCC ACTGTGGGAG ATGGACGTCA GGACAATCTT 
GAAATGGAAG ATGAGCATGT TAAGGAAGGG CCGTCGGACT CTGAGGACAT TGTGTTTGAA 
GAGGCTGACA CAGACTTACA GGAGCTCCAA GCCTCCATGG AGCAGCTGCT CCAGGAGCAG 
CCAGGGGAGG ACTACAGCGA AGAGGAGGAG TCGGGCTTGA AGACCAGTGA TGACGCCGAG 
CAGGCCGCCA ACGGCACGGA CGCGGGCGAG GAGGACGACA ACCCCAGCAG CGAGAGCGCC 
CTCAATGAGG AGTGGCACTC GGATAACAGT GATGGGGAGA TAACTAGTGA ATGTGAATAC 
GATAGTGTCT TTAATCATTT AGAGGAACTG AGGCTTCACC TGGAACAGGA AATAGGCTTC 
GAAAAGTTCT TTGAGGTTTA TGAAAAAATA AAGGCTGTTC ATGAAGATGA AGATGAAAAT 
ATTGAGATTT GTTCAACAAT AGTTCAGAAT ATTTTGGGAA ATGAACATCA GCATCTTTAT 
GCCAAGATTC TTCATTTAGT CATGGCAGAT GGAGCCTATC AAGAAGATAA TGATGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015319899.1
CDS	770..4489
Translation	MEKYIRVQKIGEGSFGKAILVRSTEDGRQYVIKEINISRMSNKEREESRREVAVLANMRHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGVLFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTRDGTIQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIISGSFPPVSLHYSYDLRSLLSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTFSKFGAQPLPAKRPASGQSLASIMSAQKITKPAAKYGIPLTNKKYIDKKLHERKPLQKYKQAHQTPVKKVNPGEERRKLFEEAPRKRRLELTEKEKKSKDQVSLLKAEQVKRQEKQRLERINRAREQGWRNVLSAGGSGEMKAPLLGSGGAMAPSSFSSRGQYEHYHAIFDQMQQQRAEDNEAKWKGEIHGQRLPERQKGQPAMERAKQVEEFLQRRREAKQNKARAEGHMGILHNLAALYGGRPSSSRGGKPRNKEEEVYLARLRQIRLQNFNERQQIRAKLRGEKKETDSSEDQEGSEEADVRRKKVEALKAQASARAAVLKEQLERKRKEAYEREKKVWEEHLVAKGIKNSDVSPPSGQQEMGGSPTKQPMRPVISVTSALKEVCMDQSLTDAQETSEEMQKSNSAVSSKREILRRLNQNLKVQENEKGRQSRRETGTVTVHEDAKELEKEKSVVSDRKKWETGGQLVLPVDELALDASFSVTEKHIVGEVIKLDPSGSPRKVWGKSPTDSVLRILGEAELQLQTELLENTSIRNEISPESENFKSLITEEEKVKCIPLEMNPPATLDSSVETNSPNISEASPQTALKAEGDLEEPDDLEAEVLQEPSGADRDGGLPQPVLDMWVTEREGAEEVELEDRITIQQNELSEDKAPSSVDHLSEVHVEPGVDDSPPSKRDIDKSMQPEPFFHKVVYSEHLKAVSQVQSVLCSPEESFPLRSHSDSPPRNKKQNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDGRQDNLEMEDEHVKEGPSDSEDIVFEEADTDLQELQASMEQLLQEQPGEDYSEEEESGLKTSDDAEQAANGTDAGEEDDNPSSESALNEEWHSDNSDGEITSECEYDSVFNHLEELRLHLEQEIGFEKFFEVYEKIKAVHEDEDENIEICSTIVQNILGNEHQHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_015464413.1
Organism	Bos taurus(cattle)
Definition	Bos taurus NIMA related kinase 1 (NEK1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015464413.1

ORF Insert Sequence:

ATGGAGAAAT ACATTAGAGT ACAGAAGATT GGAGAAGGTT CATTCGGAAA AGCCATTCTT 
GTCAGATCTA CAGAAGATGG CAGACAATAT GTCATCAAAG AAATTAATAT CTCAAGGATG 
TCCAATAAAG AGAGGGAAGA ATCAAGGAGA GAAGTTGCAG TGCTAGCAAA CATGCGGCAT 
CCAAACATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATTGTAATG 
GATTACTGTG AAGGAGGGGA TCTGTTCAAA AGAATAAATG CTCAGAAGGG CGTTTTGTTT 
CAAGAGGACC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTAAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATAAAA TCACAGAACA TATTTTTAAC CAGAGATGGA 
ACAATACAAC TTGGAGATTT TGGAATCGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA TAAACCTTAT 
AATAATAAAA GTGATATTTG GGCTCTGGGG TGTGTCCTTT ATGAGATGTG CACACTTAAA 
CATGCATTTG AAGCTGGCAA TATGAAGAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCCGTGT CTTTGCATTA TTCTTACGAT CTCCGCAGTT TACTCTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC CCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCCAAGTTTG GAGCACAACC TCTACCAGCT AAAAGACCAG CTTCAGGACA AAGCTTGGCT 
TCTATTATGT CTGCTCAGAA AATTACAAAG CCTGCTGCTA AATATGGAAT ACCGTTAACA 
AATAAGAAAT ATATAGATAA AAAATTGCAT GAAAGGAAGC CACTCCAGAA ATATAAACAG 
GCCCATCAAA CCCCAGTGAA GAAAGTGAAT CCTGGAGAAG AAAGGAGGAA ACTGTTTGAG 
GAAGCACCAA GAAAAAGAAG ATTGGAATTG ACTGAAAAAG AAAAGAAGTC TAAGGATCAG 
GTTAGTCTGC TGAAGGCTGA GCAGGTGAAA AGACAGGAAA AGCAAAGGTT GGAGAGAATA 
AATAGGGCCA GGGAACAAGG CTGGAGAAAT GTGCTGAGTG CTGGTGGAAG CGGTGAAATG 
AAGGCTCCCT TGTTGGGCAG TGGAGGGGCC ATGGCTCCAT CATCTTTTTC CTCTCGAGGA 
CAGTATGAAC ATTATCACGC TATTTTTGAT CAAATGCAGC AACAAAGAGC AGAAGATAAT 
GAAGCTAAAT GGAAAGGAGA AATACATGGC CAGAGGCTTC CAGAAAGGCA GAAGGGGCAG 
CCAGCTATGG AAAGAGCAAA ACAAGTAGAA GAGTTTCTAC AGCGAAGACG GGAAGCAAAG 
CAGAACAAAG CTCGGGCCGA GGGGCATATG GGCATCCTGC ACAACCTGGC AGCTCTCTAT 
GGAGGCAGGC CCAGCTCTTC ACGAGGAGGG AAGCCGAGAA ACAAAGAGGA AGAGGTTTAT 
TTGGCAAGAC TAAGACAAAT AAGACTGCAG AATTTCAATG AACGCCAACA GATTAGAGCC 
AAGCTTCGTG GTGAAAAGAA GGAAACTGAC AGCTCTGAAG ATCAAGAAGG AAGTGAGGAG 
GCTGATGTGA GGCGTAAAAA AGTGGAAGCC CTGAAGGCCC AGGCAAGTGC CCGTGCAGCC 
GTCCTGAAGG AGCAGCTTGA GCGCAAGCGG AAGGAGGCAT ATGAGAGAGA GAAGAAAGTG 
TGGGAGGAGC ATTTGGTGGC CAAGGGAATA AAGAACTCTG ATGTGTCTCC GCCGTCAGGG 
CAGCAGGAAA TGGGGGGTTC CCCAACAAAG CAGCCAATGA GACCTGTTAT TTCTGTGACT 
TCGGCTTTGA AGGAAGTGTG TATGGACCAA AGTCTAACTG ATGCCCAAGA AACCTCAGAA 
GAAATGCAGA AGAGTAACAG TGCTGTTTCA AGTAAACGAG AAATACTACG TAGATTAAAT 
CAAAATCTTA AAGTTCAAGA AAATGAAAAG GGAAGGCAGA GTCGTCGTGA AACGGGTACA 
GTAACTGTTC ATGAAGATGC CAAAGAGCTG GAAAAAGAAA AGTCAGTTGT ATCGGATCGA 
AAAAAATGGG AGACAGGAGG CCAGCTTGTG CTTCCTGTGG ACGAACTGGC CCTGGACGCT 
TCCTTCTCTG TGACAGAAAA ACATATAGTG GGAGAGGTTA TTAAATTAGA TCCTAGTGGA 
TCTCCAAGAA AAGTCTGGGG GAAAAGTCCT ACAGATTCTG TTCTGAGGAT ACTTGGAGAA 
GCTGAACTAC AACTTCAGAC AGAACTACTG GAAAACACAT CTATTAGAAA TGAGATTTCT 
CCTGAAAGCG AGAATTTCAA ATCCTTAATT ACTGAAGAAG AAAAAGTAAA ATGTATTCCA 
CTTGAAATGA ACCCACCAGC TACTCTTGAT TCTTCTGTTG AGACAAACAG TCCAAACATT 
AGTGAAGCAT CTCCACAGAC AGCATTGAAA GCAGAAGGAG ATTTGGAGGA GCCTGATGAC 
TTGGAGGCAG AAGTGCTGCA GGAGCCAAGT GGAGCAGACA GAGATGGTGG TTTGCCCCAA 
CCTGTTCTCG ACATGTGGGT CACTGAGAGA GAAGGAGCAG AGGAGGTTGA ATTGGAAGAT 
AGGATCACCA TTCAGCAAAA TGAACTTTCT GAAGATAAAG CCCCGAGCAG TGTGGATCAC 
CTTAGTGAGG TTCATGTAGA ACCTGGAGTG GATGATTCTC CACCCTCTAA GCGCGATATA 
GATAAATCTA TGCAACCAGA ACCATTTTTC CATAAAGTGG TTTATTCTGA GCATCTGAAA 
GCAGTTTCTC AGGTTCAGTC AGTTCTCTGT TCACCAGAAG AATCCTTTCC ACTTCGATCT 
CACTCTGATT CACCACCAAG GAATAAAAAA CAGAATTCCT TGCTGATTGG ACTTTCAACG 
GGTCTATTTG ATGCAAACAA CCCAAAGATG TTGCGGACAT GTTCACTTCC AGACCTCTCC 
AAACTGTTCA GAACACTGAT GGATGTCCCC ACTGTGGGAG ATGGACGTCA GGACAATCTT 
GAAATGGAAG ATGAGCATGT TAAGGAAGGG CCGTCGGACT CTGAGGACAT TGTGTTTGAA 
GAGGCTGACA CAGACTTACA GGAGCTCCAA GCCTCCATGG AGCAGCTGCT CCAGGAGCAG 
CCAGGGGAGG ACTACAGCGA AGAGGAGGAG TCGGGCTTGA AGACCAGTGA TGACGCCGAG 
CAGGCCGCCA ACGGCACGGA CGCGGGCGAG GAGGACGACA ACCCCAGCAG CGAGAGCGCC 
CTCAATGAGG AGTGGCACTC GGATAACAGT GATGGGGAGA TAACTAGTGA ATGTGAATAC 
GATAGTGTCT TTAATCATTT AGAGGAACTG AGGCTTCACC TGGAACAGGA AATAGGCTTC 
GAAAAGTTCT TTGAGGTTTA TGAAAAAATA AAGGCTGTTC ATGAAGATGA AGATGAAAAT 
ATTGAGATTT GTTCAACAAT AGTTCAGAAT ATTTTGGGAA ATGAACATCA GCATCTTTAT 
GCCAAGATTC TTCATTTAGT CATGGCAGAT GGAGCCTATC AAGAAGATAA TGATGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_015464414.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015319900.1 Bos taurus NIMA related kinase 1 (NEK1), transcript variant X2, mRNA.

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CloneID	OBa52910
Clone ID Related Accession (Same CDS sequence)	XM_015464414.1 , XM_010807676.2 , XM_010807676.1
Accession Version	XM_015464414.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3660bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	serine/threonine-protein kinase Nek1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645187.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAAT ACATTAGAGT ACAGAAGATT GGAGAAGGTT CATTCGGAAA AGCCATTCTT 
GTCAGATCTA CAGAAGATGG CAGACAATAT GTCATCAAAG AAATTAATAT CTCAAGGATG 
TCCAATAAAG AGAGGGAAGA ATCAAGGAGA GAAGTTGCAG TGCTAGCAAA CATGCGGCAT 
CCAAACATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATTGTAATG 
GATTACTGTG AAGGAGGGGA TCTGTTCAAA AGAATAAATG CTCAGAAGGG CGTTTTGTTT 
CAAGAGGACC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTAAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATAAAA TCACAGAACA TATTTTTAAC CAGAGATGGA 
ACAATACAAC TTGGAGATTT TGGAATCGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA TAAACCTTAT 
AATAATAAAA GTGATATTTG GGCTCTGGGG TGTGTCCTTT ATGAGATGTG CACACTTAAA 
CATGCATTTG AAGCTGGCAA TATGAAGAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCCGTGT CTTTGCATTA TTCTTACGAT CTCCGCAGTT TACTCTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC CCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCCAAGTTTG GAGCACAACC TCTACCAGCT AAAAGACCAG CTTCAGGACA AAGCTTGGCT 
TCTATTATGT CTGCTCAGAA AATTACAAAG CCTGCTGCTA AATATGGAAT ACCGTTAACA 
AATAAGAAAT ATATAGATAA AAAATTGCAT GAAAGGAAGC CACTCCAGAA ATATAAACAG 
GAAGCACCAA GAAAAAGAAG ATTGGAATTG ACTGAAAAAG AAAAGAAGTC TAAGGATCAG 
GTTAGTCTGC TGAAGGCTGA GCAGGTGAAA AGACAGGAAA AGCAAAGGTT GGAGAGAATA 
AATAGGGCCA GGGAACAAGG CTGGAGAAAT GTGCTGAGTG CTGGTGGAAG CGGTGAAATG 
AAGGCTCCCT TGTTGGGCAG TGGAGGGGCC ATGGCTCCAT CATCTTTTTC CTCTCGAGGA 
CAGTATGAAC ATTATCACGC TATTTTTGAT CAAATGCAGC AACAAAGAGC AGAAGATAAT 
GAAGCTAAAT GGAAAGGAGA AATACATGGC CAGAGGCTTC CAGAAAGGCA GAAGGGGCAG 
CCAGCTATGG AAAGAGCAAA ACAAGTAGAA GAGTTTCTAC AGCGAAGACG GGAAGCAAAG 
CAGAACAAAG CTCGGGCCGA GGGGCATATG GGCATCCTGC ACAACCTGGC AGCTCTCTAT 
GGAGGCAGGC CCAGCTCTTC ACGAGGAGGG AAGCCGAGAA ACAAAGAGGA AGAGGTTTAT 
TTGGCAAGAC TAAGACAAAT AAGACTGCAG AATTTCAATG AACGCCAACA GATTAGAGCC 
AAGCTTCGTG GTGAAAAGAA GGAAACTGAC AGCTCTGAAG ATCAAGAAGG AAGTGAGGAG 
GCTGATGTGA GGCGTAAAAA AGTGGAAGCC CTGAAGGCCC AGGCAAGTGC CCGTGCAGCC 
GTCCTGAAGG AGCAGCTTGA GCGCAAGCGG AAGGAGGCAT ATGAGAGAGA GAAGAAAGTG 
TGGGAGGAGC ATTTGGTGGC CAAGGGAATA AAGAACTCTG ATGTGTCTCC GCCGTCAGGG 
CAGCAGGAAA TGGGGGGTTC CCCAACAAAG CAGCCAATGA GACCTGTTAT TTCTGTGACT 
TCGGCTTTGA AGGAAGTGTG TATGGACCAA AGTCTAACTG ATGCCCAAGA AACCTCAGAA 
GAAATGCAGA AGAGTAACAG TGCTGTTTCA AGTAAACGAG AAATACTACG TAGATTAAAT 
CAAAATCTTA AAGTTCAAGA AAATGAAAAG GGAAGGCAGA GTCGTCGTGA AACGGGTACA 
GTAACTGTTC ATGAAGATGC CAAAGAGCTG GAAAAAGAAA AGTCAGTTGT ATCGGATCGA 
AAAAAATGGG AGACAGGAGG CCAGCTTGTG CTTCCTGTGG ACGAACTGGC CCTGGACGCT 
TCCTTCTCTG TGACAGAAAA ACATATAGTG GGAGAGGTTA TTAAATTAGA TCCTAGTGGA 
TCTCCAAGAA AAGTCTGGGG GAAAAGTCCT ACAGATTCTG TTCTGAGGAT ACTTGGAGAA 
GCTGAACTAC AACTTCAGAC AGAACTACTG GAAAACACAT CTATTAGAAA TGAGATTTCT 
CCTGAAAGCG AGAATTTCAA ATCCTTAATT ACTGAAGAAG AAAAAGTAAA ATGTATTCCA 
CTTGAAATGA ACCCACCAGC TACTCTTGAT TCTTCTGTTG AGACAAACAG TCCAAACATT 
AGTGAAGCAT CTCCACAGAC AGCATTGAAA GCAGAAGGAG ATTTGGAGGA GCCTGATGAC 
TTGGAGGCAG AAGTGCTGCA GGAGCCAAGT GGAGCAGACA GAGATGGTGG TTTGCCCCAA 
CCTGTTCTCG ACATGTGGGT CACTGAGAGA GAAGGAGCAG AGGAGGTTGA ATTGGAAGAT 
AGGATCACCA TTCAGCAAAA TGAACTTTCT GAAGATAAAG CCCCGAGCAG TGTGGATCAC 
CTTAGTGAGG TTCATGTAGA ACCTGGAGTG GATGATTCTC CACCCTCTAA GCGCGATATA 
GATAAATCTA TGCAACCAGA ACCATTTTTC CATAAAGTGG TTTATTCTGA GCATCTGAAA 
GCAGTTTCTC AGGTTCAGTC AGTTCTCTGT TCACCAGAAG AATCCTTTCC ACTTCGATCT 
CACTCTGATT CACCACCAAG GAATAAAAAA CAGAATTCCT TGCTGATTGG ACTTTCAACG 
GGTCTATTTG ATGCAAACAA CCCAAAGATG TTGCGGACAT GTTCACTTCC AGACCTCTCC 
AAACTGTTCA GAACACTGAT GGATGTCCCC ACTGTGGGAG ATGGACGTCA GGACAATCTT 
GAAATGGAAG ATGAGCATGT TAAGGAAGGG CCGTCGGACT CTGAGGACAT TGTGTTTGAA 
GAGGCTGACA CAGACTTACA GGAGCTCCAA GCCTCCATGG AGCAGCTGCT CCAGGAGCAG 
CCAGGGGAGG ACTACAGCGA AGAGGAGGAG TCGGGCTTGA AGACCAGTGA TGACGCCGAG 
CAGGCCGCCA ACGGCACGGA CGCGGGCGAG GAGGACGACA ACCCCAGCAG CGAGAGCGCC 
CTCAATGAGG AGTGGCACTC GGATAACAGT GATGGGGAGA TAACTAGTGA ATGTGAATAC 
GATAGTGTCT TTAATCATTT AGAGGAACTG AGGCTTCACC TGGAACAGGA AATAGGCTTC 
GAAAAGTTCT TTGAGGTTTA TGAAAAAATA AAGGCTGTTC ATGAAGATGA AGATGAAAAT 
ATTGAGATTT GTTCAACAAT AGTTCAGAAT ATTTTGGGAA ATGAACATCA GCATCTTTAT 
GCCAAGATTC TTCATTTAGT CATGGCAGAT GGAGCCTATC AAGAAGATAA TGATGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015319900.1
CDS	770..4429
Translation	MEKYIRVQKIGEGSFGKAILVRSTEDGRQYVIKEINISRMSNKEREESRREVAVLANMRHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGVLFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTRDGTIQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIISGSFPPVSLHYSYDLRSLLSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTFSKFGAQPLPAKRPASGQSLASIMSAQKITKPAAKYGIPLTNKKYIDKKLHERKPLQKYKQEAPRKRRLELTEKEKKSKDQVSLLKAEQVKRQEKQRLERINRAREQGWRNVLSAGGSGEMKAPLLGSGGAMAPSSFSSRGQYEHYHAIFDQMQQQRAEDNEAKWKGEIHGQRLPERQKGQPAMERAKQVEEFLQRRREAKQNKARAEGHMGILHNLAALYGGRPSSSRGGKPRNKEEEVYLARLRQIRLQNFNERQQIRAKLRGEKKETDSSEDQEGSEEADVRRKKVEALKAQASARAAVLKEQLERKRKEAYEREKKVWEEHLVAKGIKNSDVSPPSGQQEMGGSPTKQPMRPVISVTSALKEVCMDQSLTDAQETSEEMQKSNSAVSSKREILRRLNQNLKVQENEKGRQSRRETGTVTVHEDAKELEKEKSVVSDRKKWETGGQLVLPVDELALDASFSVTEKHIVGEVIKLDPSGSPRKVWGKSPTDSVLRILGEAELQLQTELLENTSIRNEISPESENFKSLITEEEKVKCIPLEMNPPATLDSSVETNSPNISEASPQTALKAEGDLEEPDDLEAEVLQEPSGADRDGGLPQPVLDMWVTEREGAEEVELEDRITIQQNELSEDKAPSSVDHLSEVHVEPGVDDSPPSKRDIDKSMQPEPFFHKVVYSEHLKAVSQVQSVLCSPEESFPLRSHSDSPPRNKKQNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDGRQDNLEMEDEHVKEGPSDSEDIVFEEADTDLQELQASMEQLLQEQPGEDYSEEEESGLKTSDDAEQAANGTDAGEEDDNPSSESALNEEWHSDNSDGEITSECEYDSVFNHLEELRLHLEQEIGFEKFFEVYEKIKAVHEDEDENIEICSTIVQNILGNEHQHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_015464414.1
Organism	Bos taurus(cattle)
Definition	Bos taurus NIMA related kinase 1 (NEK1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015464414.1

ORF Insert Sequence:

ATGGAGAAAT ACATTAGAGT ACAGAAGATT GGAGAAGGTT CATTCGGAAA AGCCATTCTT 
GTCAGATCTA CAGAAGATGG CAGACAATAT GTCATCAAAG AAATTAATAT CTCAAGGATG 
TCCAATAAAG AGAGGGAAGA ATCAAGGAGA GAAGTTGCAG TGCTAGCAAA CATGCGGCAT 
CCAAACATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATTGTAATG 
GATTACTGTG AAGGAGGGGA TCTGTTCAAA AGAATAAATG CTCAGAAGGG CGTTTTGTTT 
CAAGAGGACC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTAAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATAAAA TCACAGAACA TATTTTTAAC CAGAGATGGA 
ACAATACAAC TTGGAGATTT TGGAATCGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA TAAACCTTAT 
AATAATAAAA GTGATATTTG GGCTCTGGGG TGTGTCCTTT ATGAGATGTG CACACTTAAA 
CATGCATTTG AAGCTGGCAA TATGAAGAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCCGTGT CTTTGCATTA TTCTTACGAT CTCCGCAGTT TACTCTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC CCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCCAAGTTTG GAGCACAACC TCTACCAGCT AAAAGACCAG CTTCAGGACA AAGCTTGGCT 
TCTATTATGT CTGCTCAGAA AATTACAAAG CCTGCTGCTA AATATGGAAT ACCGTTAACA 
AATAAGAAAT ATATAGATAA AAAATTGCAT GAAAGGAAGC CACTCCAGAA ATATAAACAG 
GAAGCACCAA GAAAAAGAAG ATTGGAATTG ACTGAAAAAG AAAAGAAGTC TAAGGATCAG 
GTTAGTCTGC TGAAGGCTGA GCAGGTGAAA AGACAGGAAA AGCAAAGGTT GGAGAGAATA 
AATAGGGCCA GGGAACAAGG CTGGAGAAAT GTGCTGAGTG CTGGTGGAAG CGGTGAAATG 
AAGGCTCCCT TGTTGGGCAG TGGAGGGGCC ATGGCTCCAT CATCTTTTTC CTCTCGAGGA 
CAGTATGAAC ATTATCACGC TATTTTTGAT CAAATGCAGC AACAAAGAGC AGAAGATAAT 
GAAGCTAAAT GGAAAGGAGA AATACATGGC CAGAGGCTTC CAGAAAGGCA GAAGGGGCAG 
CCAGCTATGG AAAGAGCAAA ACAAGTAGAA GAGTTTCTAC AGCGAAGACG GGAAGCAAAG 
CAGAACAAAG CTCGGGCCGA GGGGCATATG GGCATCCTGC ACAACCTGGC AGCTCTCTAT 
GGAGGCAGGC CCAGCTCTTC ACGAGGAGGG AAGCCGAGAA ACAAAGAGGA AGAGGTTTAT 
TTGGCAAGAC TAAGACAAAT AAGACTGCAG AATTTCAATG AACGCCAACA GATTAGAGCC 
AAGCTTCGTG GTGAAAAGAA GGAAACTGAC AGCTCTGAAG ATCAAGAAGG AAGTGAGGAG 
GCTGATGTGA GGCGTAAAAA AGTGGAAGCC CTGAAGGCCC AGGCAAGTGC CCGTGCAGCC 
GTCCTGAAGG AGCAGCTTGA GCGCAAGCGG AAGGAGGCAT ATGAGAGAGA GAAGAAAGTG 
TGGGAGGAGC ATTTGGTGGC CAAGGGAATA AAGAACTCTG ATGTGTCTCC GCCGTCAGGG 
CAGCAGGAAA TGGGGGGTTC CCCAACAAAG CAGCCAATGA GACCTGTTAT TTCTGTGACT 
TCGGCTTTGA AGGAAGTGTG TATGGACCAA AGTCTAACTG ATGCCCAAGA AACCTCAGAA 
GAAATGCAGA AGAGTAACAG TGCTGTTTCA AGTAAACGAG AAATACTACG TAGATTAAAT 
CAAAATCTTA AAGTTCAAGA AAATGAAAAG GGAAGGCAGA GTCGTCGTGA AACGGGTACA 
GTAACTGTTC ATGAAGATGC CAAAGAGCTG GAAAAAGAAA AGTCAGTTGT ATCGGATCGA 
AAAAAATGGG AGACAGGAGG CCAGCTTGTG CTTCCTGTGG ACGAACTGGC CCTGGACGCT 
TCCTTCTCTG TGACAGAAAA ACATATAGTG GGAGAGGTTA TTAAATTAGA TCCTAGTGGA 
TCTCCAAGAA AAGTCTGGGG GAAAAGTCCT ACAGATTCTG TTCTGAGGAT ACTTGGAGAA 
GCTGAACTAC AACTTCAGAC AGAACTACTG GAAAACACAT CTATTAGAAA TGAGATTTCT 
CCTGAAAGCG AGAATTTCAA ATCCTTAATT ACTGAAGAAG AAAAAGTAAA ATGTATTCCA 
CTTGAAATGA ACCCACCAGC TACTCTTGAT TCTTCTGTTG AGACAAACAG TCCAAACATT 
AGTGAAGCAT CTCCACAGAC AGCATTGAAA GCAGAAGGAG ATTTGGAGGA GCCTGATGAC 
TTGGAGGCAG AAGTGCTGCA GGAGCCAAGT GGAGCAGACA GAGATGGTGG TTTGCCCCAA 
CCTGTTCTCG ACATGTGGGT CACTGAGAGA GAAGGAGCAG AGGAGGTTGA ATTGGAAGAT 
AGGATCACCA TTCAGCAAAA TGAACTTTCT GAAGATAAAG CCCCGAGCAG TGTGGATCAC 
CTTAGTGAGG TTCATGTAGA ACCTGGAGTG GATGATTCTC CACCCTCTAA GCGCGATATA 
GATAAATCTA TGCAACCAGA ACCATTTTTC CATAAAGTGG TTTATTCTGA GCATCTGAAA 
GCAGTTTCTC AGGTTCAGTC AGTTCTCTGT TCACCAGAAG AATCCTTTCC ACTTCGATCT 
CACTCTGATT CACCACCAAG GAATAAAAAA CAGAATTCCT TGCTGATTGG ACTTTCAACG 
GGTCTATTTG ATGCAAACAA CCCAAAGATG TTGCGGACAT GTTCACTTCC AGACCTCTCC 
AAACTGTTCA GAACACTGAT GGATGTCCCC ACTGTGGGAG ATGGACGTCA GGACAATCTT 
GAAATGGAAG ATGAGCATGT TAAGGAAGGG CCGTCGGACT CTGAGGACAT TGTGTTTGAA 
GAGGCTGACA CAGACTTACA GGAGCTCCAA GCCTCCATGG AGCAGCTGCT CCAGGAGCAG 
CCAGGGGAGG ACTACAGCGA AGAGGAGGAG TCGGGCTTGA AGACCAGTGA TGACGCCGAG 
CAGGCCGCCA ACGGCACGGA CGCGGGCGAG GAGGACGACA ACCCCAGCAG CGAGAGCGCC 
CTCAATGAGG AGTGGCACTC GGATAACAGT GATGGGGAGA TAACTAGTGA ATGTGAATAC 
GATAGTGTCT TTAATCATTT AGAGGAACTG AGGCTTCACC TGGAACAGGA AATAGGCTTC 
GAAAAGTTCT TTGAGGTTTA TGAAAAAATA AAGGCTGTTC ATGAAGATGA AGATGAAAAT 
ATTGAGATTT GTTCAACAAT AGTTCAGAAT ATTTTGGGAA ATGAACATCA GCATCTTTAT 
GCCAAGATTC TTCATTTAGT CATGGCAGAT GGAGCCTATC AAGAAGATAA TGATGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_010807676.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010805978.1 Bos taurus NIMA related kinase 1 (NEK1), transcript variant X2, mRNA.

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CloneID	OBa52910
Clone ID Related Accession (Same CDS sequence)	XM_015464414.1 , XM_010807676.2 , XM_010807676.1
Accession Version	XM_010807676.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3660bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	serine/threonine-protein kinase Nek1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010807676.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAAT ACATTAGAGT ACAGAAGATT GGAGAAGGTT CATTCGGAAA AGCCATTCTT 
GTCAGATCTA CAGAAGATGG CAGACAATAT GTCATCAAAG AAATTAATAT CTCAAGGATG 
TCCAATAAAG AGAGGGAAGA ATCAAGGAGA GAAGTTGCAG TGCTAGCAAA CATGCGGCAT 
CCAAACATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATTGTAATG 
GATTACTGTG AAGGAGGGGA TCTGTTCAAA AGAATAAATG CTCAGAAGGG CGTTTTGTTT 
CAAGAGGACC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTAAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATAAAA TCACAGAACA TATTTTTAAC CAGAGATGGA 
ACAATACAAC TTGGAGATTT TGGAATCGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA TAAACCTTAT 
AATAATAAAA GTGATATTTG GGCTCTGGGG TGTGTCCTTT ATGAGATGTG CACACTTAAA 
CATGCATTTG AAGCTGGCAA TATGAAGAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCCGTGT CTTTGCATTA TTCTTACGAT CTCCGCAGTT TACTCTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC CCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCCAAGTTTG GAGCACAACC TCTACCAGCT AAAAGACCAG CTTCAGGACA AAGCTTGGCT 
TCTATTATGT CTGCTCAGAA AATTACAAAG CCTGCTGCTA AATATGGAAT ACCGTTAACA 
AATAAGAAAT ATATAGATAA AAAATTGCAT GAAAGGAAGC CACTCCAGAA ATATAAACAG 
GAAGCACCAA GAAAAAGAAG ATTGGAATTG ACTGAAAAAG AAAAGAAGTC TAAGGATCAG 
GTTAGTCTGC TGAAGGCTGA GCAGGTGAAA AGACAGGAAA AGCAAAGGTT GGAGAGAATA 
AATAGGGCCA GGGAACAAGG CTGGAGAAAT GTGCTGAGTG CTGGTGGAAG CGGTGAAATG 
AAGGCTCCCT TGTTGGGCAG TGGAGGGGCC ATGGCTCCAT CATCTTTTTC CTCTCGAGGA 
CAGTATGAAC ATTATCACGC TATTTTTGAT CAAATGCAGC AACAAAGAGC AGAAGATAAT 
GAAGCTAAAT GGAAAGGAGA AATACATGGC CAGAGGCTTC CAGAAAGGCA GAAGGGGCAG 
CCAGCTATGG AAAGAGCAAA ACAAGTAGAA GAGTTTCTAC AGCGAAGACG GGAAGCAAAG 
CAGAACAAAG CTCGGGCCGA GGGGCATATG GGCATCCTGC ACAACCTGGC AGCTCTCTAT 
GGAGGCAGGC CCAGCTCTTC ACGAGGAGGG AAGCCGAGAA ACAAAGAGGA AGAGGTTTAT 
TTGGCAAGAC TAAGACAAAT AAGACTGCAG AATTTCAATG AACGCCAACA GATTAGAGCC 
AAGCTTCGTG GTGAAAAGAA GGAAACTGAC AGCTCTGAAG ATCAAGAAGG AAGTGAGGAG 
GCTGATGTGA GGCGTAAAAA AGTGGAAGCC CTGAAGGCCC AGGCAAGTGC CCGTGCAGCC 
GTCCTGAAGG AGCAGCTTGA GCGCAAGCGG AAGGAGGCAT ATGAGAGAGA GAAGAAAGTG 
TGGGAGGAGC ATTTGGTGGC CAAGGGAATA AAGAACTCTG ATGTGTCTCC GCCGTCAGGG 
CAGCAGGAAA TGGGGGGTTC CCCAACAAAG CAGCCAATGA GACCTGTTAT TTCTGTGACT 
TCGGCTTTGA AGGAAGTGTG TATGGACCAA AGTCTAACTG ATGCCCAAGA AACCTCAGAA 
GAAATGCAGA AGAGTAACAG TGCTGTTTCA AGTAAACGAG AAATACTACG TAGATTAAAT 
CAAAATCTTA AAGTTCAAGA AAATGAAAAG GGAAGGCAGA GTCGTCGTGA AACGGGTACA 
GTAACTGTTC ATGAAGATGC CAAAGAGCTG GAAAAAGAAA AGTCAGTTGT ATCGGATCGA 
AAAAAATGGG AGACAGGAGG CCAGCTTGTG CTTCCTGTGG ACGAACTGGC CCTGGACGCT 
TCCTTCTCTG TGACAGAAAA ACATATAGTG GGAGAGGTTA TTAAATTAGA TCCTAGTGGA 
TCTCCAAGAA AAGTCTGGGG GAAAAGTCCT ACAGATTCTG TTCTGAGGAT ACTTGGAGAA 
GCTGAACTAC AACTTCAGAC AGAACTACTG GAAAACACAT CTATTAGAAA TGAGATTTCT 
CCTGAAAGCG AGAATTTCAA ATCCTTAATT ACTGAAGAAG AAAAAGTAAA ATGTATTCCA 
CTTGAAATGA ACCCACCAGC TACTCTTGAT TCTTCTGTTG AGACAAACAG TCCAAACATT 
AGTGAAGCAT CTCCACAGAC AGCATTGAAA GCAGAAGGAG ATTTGGAGGA GCCTGATGAC 
TTGGAGGCAG AAGTGCTGCA GGAGCCAAGT GGAGCAGACA GAGATGGTGG TTTGCCCCAA 
CCTGTTCTCG ACATGTGGGT CACTGAGAGA GAAGGAGCAG AGGAGGTTGA ATTGGAAGAT 
AGGATCACCA TTCAGCAAAA TGAACTTTCT GAAGATAAAG CCCCGAGCAG TGTGGATCAC 
CTTAGTGAGG TTCATGTAGA ACCTGGAGTG GATGATTCTC CACCCTCTAA GCGCGATATA 
GATAAATCTA TGCAACCAGA ACCATTTTTC CATAAAGTGG TTTATTCTGA GCATCTGAAA 
GCAGTTTCTC AGGTTCAGTC AGTTCTCTGT TCACCAGAAG AATCCTTTCC ACTTCGATCT 
CACTCTGATT CACCACCAAG GAATAAAAAA CAGAATTCCT TGCTGATTGG ACTTTCAACG 
GGTCTATTTG ATGCAAACAA CCCAAAGATG TTGCGGACAT GTTCACTTCC AGACCTCTCC 
AAACTGTTCA GAACACTGAT GGATGTCCCC ACTGTGGGAG ATGGACGTCA GGACAATCTT 
GAAATGGAAG ATGAGCATGT TAAGGAAGGG CCGTCGGACT CTGAGGACAT TGTGTTTGAA 
GAGGCTGACA CAGACTTACA GGAGCTCCAA GCCTCCATGG AGCAGCTGCT CCAGGAGCAG 
CCAGGGGAGG ACTACAGCGA AGAGGAGGAG TCGGGCTTGA AGACCAGTGA TGACGCCGAG 
CAGGCCGCCA ACGGCACGGA CGCGGGCGAG GAGGACGACA ACCCCAGCAG CGAGAGCGCC 
CTCAATGAGG AGTGGCACTC GGATAACAGT GATGGGGAGA TAACTAGTGA ATGTGAATAC 
GATAGTGTCT TTAATCATTT AGAGGAACTG AGGCTTCACC TGGAACAGGA AATAGGCTTC 
GAAAAGTTCT TTGAGGTTTA TGAAAAAATA AAGGCTGTTC ATGAAGATGA AGATGAAAAT 
ATTGAGATTT GTTCAACAAT AGTTCAGAAT ATTTTGGGAA ATGAACATCA GCATCTTTAT 
GCCAAGATTC TTCATTTAGT CATGGCAGAT GGAGCCTATC AAGAAGATAA TGATGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010805978.1
CDS	1123..4782
Translation	MEKYIRVQKIGEGSFGKAILVRSTEDGRQYVIKEINISRMSNKEREESRREVAVLANMRHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGVLFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTRDGTIQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIISGSFPPVSLHYSYDLRSLLSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTFSKFGAQPLPAKRPASGQSLASIMSAQKITKPAAKYGIPLTNKKYIDKKLHERKPLQKYKQEAPRKRRLELTEKEKKSKDQVSLLKAEQVKRQEKQRLERINRAREQGWRNVLSAGGSGEMKAPLLGSGGAMAPSSFSSRGQYEHYHAIFDQMQQQRAEDNEAKWKGEIHGQRLPERQKGQPAMERAKQVEEFLQRRREAKQNKARAEGHMGILHNLAALYGGRPSSSRGGKPRNKEEEVYLARLRQIRLQNFNERQQIRAKLRGEKKETDSSEDQEGSEEADVRRKKVEALKAQASARAAVLKEQLERKRKEAYEREKKVWEEHLVAKGIKNSDVSPPSGQQEMGGSPTKQPMRPVISVTSALKEVCMDQSLTDAQETSEEMQKSNSAVSSKREILRRLNQNLKVQENEKGRQSRRETGTVTVHEDAKELEKEKSVVSDRKKWETGGQLVLPVDELALDASFSVTEKHIVGEVIKLDPSGSPRKVWGKSPTDSVLRILGEAELQLQTELLENTSIRNEISPESENFKSLITEEEKVKCIPLEMNPPATLDSSVETNSPNISEASPQTALKAEGDLEEPDDLEAEVLQEPSGADRDGGLPQPVLDMWVTEREGAEEVELEDRITIQQNELSEDKAPSSVDHLSEVHVEPGVDDSPPSKRDIDKSMQPEPFFHKVVYSEHLKAVSQVQSVLCSPEESFPLRSHSDSPPRNKKQNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDGRQDNLEMEDEHVKEGPSDSEDIVFEEADTDLQELQASMEQLLQEQPGEDYSEEEESGLKTSDDAEQAANGTDAGEEDDNPSSESALNEEWHSDNSDGEITSECEYDSVFNHLEELRLHLEQEIGFEKFFEVYEKIKAVHEDEDENIEICSTIVQNILGNEHQHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_010807676.2
Organism	Bos taurus(cattle)
Definition	Bos taurus NIMA related kinase 1 (NEK1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010807676.2

ORF Insert Sequence:

ATGGAGAAAT ACATTAGAGT ACAGAAGATT GGAGAAGGTT CATTCGGAAA AGCCATTCTT 
GTCAGATCTA CAGAAGATGG CAGACAATAT GTCATCAAAG AAATTAATAT CTCAAGGATG 
TCCAATAAAG AGAGGGAAGA ATCAAGGAGA GAAGTTGCAG TGCTAGCAAA CATGCGGCAT 
CCAAACATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATTGTAATG 
GATTACTGTG AAGGAGGGGA TCTGTTCAAA AGAATAAATG CTCAGAAGGG CGTTTTGTTT 
CAAGAGGACC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTAAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATAAAA TCACAGAACA TATTTTTAAC CAGAGATGGA 
ACAATACAAC TTGGAGATTT TGGAATCGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA TAAACCTTAT 
AATAATAAAA GTGATATTTG GGCTCTGGGG TGTGTCCTTT ATGAGATGTG CACACTTAAA 
CATGCATTTG AAGCTGGCAA TATGAAGAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCCGTGT CTTTGCATTA TTCTTACGAT CTCCGCAGTT TACTCTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC CCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCCAAGTTTG GAGCACAACC TCTACCAGCT AAAAGACCAG CTTCAGGACA AAGCTTGGCT 
TCTATTATGT CTGCTCAGAA AATTACAAAG CCTGCTGCTA AATATGGAAT ACCGTTAACA 
AATAAGAAAT ATATAGATAA AAAATTGCAT GAAAGGAAGC CACTCCAGAA ATATAAACAG 
GAAGCACCAA GAAAAAGAAG ATTGGAATTG ACTGAAAAAG AAAAGAAGTC TAAGGATCAG 
GTTAGTCTGC TGAAGGCTGA GCAGGTGAAA AGACAGGAAA AGCAAAGGTT GGAGAGAATA 
AATAGGGCCA GGGAACAAGG CTGGAGAAAT GTGCTGAGTG CTGGTGGAAG CGGTGAAATG 
AAGGCTCCCT TGTTGGGCAG TGGAGGGGCC ATGGCTCCAT CATCTTTTTC CTCTCGAGGA 
CAGTATGAAC ATTATCACGC TATTTTTGAT CAAATGCAGC AACAAAGAGC AGAAGATAAT 
GAAGCTAAAT GGAAAGGAGA AATACATGGC CAGAGGCTTC CAGAAAGGCA GAAGGGGCAG 
CCAGCTATGG AAAGAGCAAA ACAAGTAGAA GAGTTTCTAC AGCGAAGACG GGAAGCAAAG 
CAGAACAAAG CTCGGGCCGA GGGGCATATG GGCATCCTGC ACAACCTGGC AGCTCTCTAT 
GGAGGCAGGC CCAGCTCTTC ACGAGGAGGG AAGCCGAGAA ACAAAGAGGA AGAGGTTTAT 
TTGGCAAGAC TAAGACAAAT AAGACTGCAG AATTTCAATG AACGCCAACA GATTAGAGCC 
AAGCTTCGTG GTGAAAAGAA GGAAACTGAC AGCTCTGAAG ATCAAGAAGG AAGTGAGGAG 
GCTGATGTGA GGCGTAAAAA AGTGGAAGCC CTGAAGGCCC AGGCAAGTGC CCGTGCAGCC 
GTCCTGAAGG AGCAGCTTGA GCGCAAGCGG AAGGAGGCAT ATGAGAGAGA GAAGAAAGTG 
TGGGAGGAGC ATTTGGTGGC CAAGGGAATA AAGAACTCTG ATGTGTCTCC GCCGTCAGGG 
CAGCAGGAAA TGGGGGGTTC CCCAACAAAG CAGCCAATGA GACCTGTTAT TTCTGTGACT 
TCGGCTTTGA AGGAAGTGTG TATGGACCAA AGTCTAACTG ATGCCCAAGA AACCTCAGAA 
GAAATGCAGA AGAGTAACAG TGCTGTTTCA AGTAAACGAG AAATACTACG TAGATTAAAT 
CAAAATCTTA AAGTTCAAGA AAATGAAAAG GGAAGGCAGA GTCGTCGTGA AACGGGTACA 
GTAACTGTTC ATGAAGATGC CAAAGAGCTG GAAAAAGAAA AGTCAGTTGT ATCGGATCGA 
AAAAAATGGG AGACAGGAGG CCAGCTTGTG CTTCCTGTGG ACGAACTGGC CCTGGACGCT 
TCCTTCTCTG TGACAGAAAA ACATATAGTG GGAGAGGTTA TTAAATTAGA TCCTAGTGGA 
TCTCCAAGAA AAGTCTGGGG GAAAAGTCCT ACAGATTCTG TTCTGAGGAT ACTTGGAGAA 
GCTGAACTAC AACTTCAGAC AGAACTACTG GAAAACACAT CTATTAGAAA TGAGATTTCT 
CCTGAAAGCG AGAATTTCAA ATCCTTAATT ACTGAAGAAG AAAAAGTAAA ATGTATTCCA 
CTTGAAATGA ACCCACCAGC TACTCTTGAT TCTTCTGTTG AGACAAACAG TCCAAACATT 
AGTGAAGCAT CTCCACAGAC AGCATTGAAA GCAGAAGGAG ATTTGGAGGA GCCTGATGAC 
TTGGAGGCAG AAGTGCTGCA GGAGCCAAGT GGAGCAGACA GAGATGGTGG TTTGCCCCAA 
CCTGTTCTCG ACATGTGGGT CACTGAGAGA GAAGGAGCAG AGGAGGTTGA ATTGGAAGAT 
AGGATCACCA TTCAGCAAAA TGAACTTTCT GAAGATAAAG CCCCGAGCAG TGTGGATCAC 
CTTAGTGAGG TTCATGTAGA ACCTGGAGTG GATGATTCTC CACCCTCTAA GCGCGATATA 
GATAAATCTA TGCAACCAGA ACCATTTTTC CATAAAGTGG TTTATTCTGA GCATCTGAAA 
GCAGTTTCTC AGGTTCAGTC AGTTCTCTGT TCACCAGAAG AATCCTTTCC ACTTCGATCT 
CACTCTGATT CACCACCAAG GAATAAAAAA CAGAATTCCT TGCTGATTGG ACTTTCAACG 
GGTCTATTTG ATGCAAACAA CCCAAAGATG TTGCGGACAT GTTCACTTCC AGACCTCTCC 
AAACTGTTCA GAACACTGAT GGATGTCCCC ACTGTGGGAG ATGGACGTCA GGACAATCTT 
GAAATGGAAG ATGAGCATGT TAAGGAAGGG CCGTCGGACT CTGAGGACAT TGTGTTTGAA 
GAGGCTGACA CAGACTTACA GGAGCTCCAA GCCTCCATGG AGCAGCTGCT CCAGGAGCAG 
CCAGGGGAGG ACTACAGCGA AGAGGAGGAG TCGGGCTTGA AGACCAGTGA TGACGCCGAG 
CAGGCCGCCA ACGGCACGGA CGCGGGCGAG GAGGACGACA ACCCCAGCAG CGAGAGCGCC 
CTCAATGAGG AGTGGCACTC GGATAACAGT GATGGGGAGA TAACTAGTGA ATGTGAATAC 
GATAGTGTCT TTAATCATTT AGAGGAACTG AGGCTTCACC TGGAACAGGA AATAGGCTTC 
GAAAAGTTCT TTGAGGTTTA TGAAAAAATA AAGGCTGTTC ATGAAGATGA AGATGAAAAT 
ATTGAGATTT GTTCAACAAT AGTTCAGAAT ATTTTGGGAA ATGAACATCA GCATCTTTAT 
GCCAAGATTC TTCATTTAGT CATGGCAGAT GGAGCCTATC AAGAAGATAA TGATGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_010807676.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010805978.1 Bos taurus NIMA related kinase 1 (NEK1), transcript variant X2, mRNA.

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CloneID	OBa52910
Clone ID Related Accession (Same CDS sequence)	XM_015464414.1 , XM_010807676.2 , XM_010807676.1
Accession Version	XM_010807676.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3660bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	serine/threonine-protein kinase Nek1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104069.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAAT ACATTAGAGT ACAGAAGATT GGAGAAGGTT CATTCGGAAA AGCCATTCTT 
GTCAGATCTA CAGAAGATGG CAGACAATAT GTCATCAAAG AAATTAATAT CTCAAGGATG 
TCCAATAAAG AGAGGGAAGA ATCAAGGAGA GAAGTTGCAG TGCTAGCAAA CATGCGGCAT 
CCAAACATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATTGTAATG 
GATTACTGTG AAGGAGGGGA TCTGTTCAAA AGAATAAATG CTCAGAAGGG CGTTTTGTTT 
CAAGAGGACC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTAAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATAAAA TCACAGAACA TATTTTTAAC CAGAGATGGA 
ACAATACAAC TTGGAGATTT TGGAATCGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA TAAACCTTAT 
AATAATAAAA GTGATATTTG GGCTCTGGGG TGTGTCCTTT ATGAGATGTG CACACTTAAA 
CATGCATTTG AAGCTGGCAA TATGAAGAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCCGTGT CTTTGCATTA TTCTTACGAT CTCCGCAGTT TACTCTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC CCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCCAAGTTTG GAGCACAACC TCTACCAGCT AAAAGACCAG CTTCAGGACA AAGCTTGGCT 
TCTATTATGT CTGCTCAGAA AATTACAAAG CCTGCTGCTA AATATGGAAT ACCGTTAACA 
AATAAGAAAT ATATAGATAA AAAATTGCAT GAAAGGAAGC CACTCCAGAA ATATAAACAG 
GAAGCACCAA GAAAAAGAAG ATTGGAATTG ACTGAAAAAG AAAAGAAGTC TAAGGATCAG 
GTTAGTCTGC TGAAGGCTGA GCAGGTGAAA AGACAGGAAA AGCAAAGGTT GGAGAGAATA 
AATAGGGCCA GGGAACAAGG CTGGAGAAAT GTGCTGAGTG CTGGTGGAAG CGGTGAAATG 
AAGGCTCCCT TGTTGGGCAG TGGAGGGGCC ATGGCTCCAT CATCTTTTTC CTCTCGAGGA 
CAGTATGAAC ATTATCACGC TATTTTTGAT CAAATGCAGC AACAAAGAGC AGAAGATAAT 
GAAGCTAAAT GGAAAGGAGA AATACATGGC CAGAGGCTTC CAGAAAGGCA GAAGGGGCAG 
CCAGCTATGG AAAGAGCAAA ACAAGTAGAA GAGTTTCTAC AGCGAAGACG GGAAGCAAAG 
CAGAACAAAG CTCGGGCCGA GGGGCATATG GGCATCCTGC ACAACCTGGC AGCTCTCTAT 
GGAGGCAGGC CCAGCTCTTC ACGAGGAGGG AAGCCGAGAA ACAAAGAGGA AGAGGTTTAT 
TTGGCAAGAC TAAGACAAAT AAGACTGCAG AATTTCAATG AACGCCAACA GATTAGAGCC 
AAGCTTCGTG GTGAAAAGAA GGAAACTGAC AGCTCTGAAG ATCAAGAAGG AAGTGAGGAG 
GCTGATGTGA GGCGTAAAAA AGTGGAAGCC CTGAAGGCCC AGGCAAGTGC CCGTGCAGCC 
GTCCTGAAGG AGCAGCTTGA GCGCAAGCGG AAGGAGGCAT ATGAGAGAGA GAAGAAAGTG 
TGGGAGGAGC ATTTGGTGGC CAAGGGAATA AAGAACTCTG ATGTGTCTCC GCCGTCAGGG 
CAGCAGGAAA TGGGGGGTTC CCCAACAAAG CAGCCAATGA GACCTGTTAT TTCTGTGACT 
TCGGCTTTGA AGGAAGTGTG TATGGACCAA AGTCTAACTG ATGCCCAAGA AACCTCAGAA 
GAAATGCAGA AGAGTAACAG TGCTGTTTCA AGTAAACGAG AAATACTACG TAGATTAAAT 
CAAAATCTTA AAGTTCAAGA AAATGAAAAG GGAAGGCAGA GTCGTCGTGA AACGGGTACA 
GTAACTGTTC ATGAAGATGC CAAAGAGCTG GAAAAAGAAA AGTCAGTTGT ATCGGATCGA 
AAAAAATGGG AGACAGGAGG CCAGCTTGTG CTTCCTGTGG ACGAACTGGC CCTGGACGCT 
TCCTTCTCTG TGACAGAAAA ACATATAGTG GGAGAGGTTA TTAAATTAGA TCCTAGTGGA 
TCTCCAAGAA AAGTCTGGGG GAAAAGTCCT ACAGATTCTG TTCTGAGGAT ACTTGGAGAA 
GCTGAACTAC AACTTCAGAC AGAACTACTG GAAAACACAT CTATTAGAAA TGAGATTTCT 
CCTGAAAGCG AGAATTTCAA ATCCTTAATT ACTGAAGAAG AAAAAGTAAA ATGTATTCCA 
CTTGAAATGA ACCCACCAGC TACTCTTGAT TCTTCTGTTG AGACAAACAG TCCAAACATT 
AGTGAAGCAT CTCCACAGAC AGCATTGAAA GCAGAAGGAG ATTTGGAGGA GCCTGATGAC 
TTGGAGGCAG AAGTGCTGCA GGAGCCAAGT GGAGCAGACA GAGATGGTGG TTTGCCCCAA 
CCTGTTCTCG ACATGTGGGT CACTGAGAGA GAAGGAGCAG AGGAGGTTGA ATTGGAAGAT 
AGGATCACCA TTCAGCAAAA TGAACTTTCT GAAGATAAAG CCCCGAGCAG TGTGGATCAC 
CTTAGTGAGG TTCATGTAGA ACCTGGAGTG GATGATTCTC CACCCTCTAA GCGCGATATA 
GATAAATCTA TGCAACCAGA ACCATTTTTC CATAAAGTGG TTTATTCTGA GCATCTGAAA 
GCAGTTTCTC AGGTTCAGTC AGTTCTCTGT TCACCAGAAG AATCCTTTCC ACTTCGATCT 
CACTCTGATT CACCACCAAG GAATAAAAAA CAGAATTCCT TGCTGATTGG ACTTTCAACG 
GGTCTATTTG ATGCAAACAA CCCAAAGATG TTGCGGACAT GTTCACTTCC AGACCTCTCC 
AAACTGTTCA GAACACTGAT GGATGTCCCC ACTGTGGGAG ATGGACGTCA GGACAATCTT 
GAAATGGAAG ATGAGCATGT TAAGGAAGGG CCGTCGGACT CTGAGGACAT TGTGTTTGAA 
GAGGCTGACA CAGACTTACA GGAGCTCCAA GCCTCCATGG AGCAGCTGCT CCAGGAGCAG 
CCAGGGGAGG ACTACAGCGA AGAGGAGGAG TCGGGCTTGA AGACCAGTGA TGACGCCGAG 
CAGGCCGCCA ACGGCACGGA CGCGGGCGAG GAGGACGACA ACCCCAGCAG CGAGAGCGCC 
CTCAATGAGG AGTGGCACTC GGATAACAGT GATGGGGAGA TAACTAGTGA ATGTGAATAC 
GATAGTGTCT TTAATCATTT AGAGGAACTG AGGCTTCACC TGGAACAGGA AATAGGCTTC 
GAAAAGTTCT TTGAGGTTTA TGAAAAAATA AAGGCTGTTC ATGAAGATGA AGATGAAAAT 
ATTGAGATTT GTTCAACAAT AGTTCAGAAT ATTTTGGGAA ATGAACATCA GCATCTTTAT 
GCCAAGATTC TTCATTTAGT CATGGCAGAT GGAGCCTATC AAGAAGATAA TGATGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010805978.1
CDS	770..4429
Translation	MEKYIRVQKIGEGSFGKAILVRSTEDGRQYVIKEINISRMSNKEREESRREVAVLANMRHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGVLFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTRDGTIQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIISGSFPPVSLHYSYDLRSLLSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTFSKFGAQPLPAKRPASGQSLASIMSAQKITKPAAKYGIPLTNKKYIDKKLHERKPLQKYKQEAPRKRRLELTEKEKKSKDQVSLLKAEQVKRQEKQRLERINRAREQGWRNVLSAGGSGEMKAPLLGSGGAMAPSSFSSRGQYEHYHAIFDQMQQQRAEDNEAKWKGEIHGQRLPERQKGQPAMERAKQVEEFLQRRREAKQNKARAEGHMGILHNLAALYGGRPSSSRGGKPRNKEEEVYLARLRQIRLQNFNERQQIRAKLRGEKKETDSSEDQEGSEEADVRRKKVEALKAQASARAAVLKEQLERKRKEAYEREKKVWEEHLVAKGIKNSDVSPPSGQQEMGGSPTKQPMRPVISVTSALKEVCMDQSLTDAQETSEEMQKSNSAVSSKREILRRLNQNLKVQENEKGRQSRRETGTVTVHEDAKELEKEKSVVSDRKKWETGGQLVLPVDELALDASFSVTEKHIVGEVIKLDPSGSPRKVWGKSPTDSVLRILGEAELQLQTELLENTSIRNEISPESENFKSLITEEEKVKCIPLEMNPPATLDSSVETNSPNISEASPQTALKAEGDLEEPDDLEAEVLQEPSGADRDGGLPQPVLDMWVTEREGAEEVELEDRITIQQNELSEDKAPSSVDHLSEVHVEPGVDDSPPSKRDIDKSMQPEPFFHKVVYSEHLKAVSQVQSVLCSPEESFPLRSHSDSPPRNKKQNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDGRQDNLEMEDEHVKEGPSDSEDIVFEEADTDLQELQASMEQLLQEQPGEDYSEEEESGLKTSDDAEQAANGTDAGEEDDNPSSESALNEEWHSDNSDGEITSECEYDSVFNHLEELRLHLEQEIGFEKFFEVYEKIKAVHEDEDENIEICSTIVQNILGNEHQHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_010807676.1
Organism	Bos taurus(cattle)
Definition	Bos taurus NIMA related kinase 1 (NEK1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010807676.1

ORF Insert Sequence:

ATGGAGAAAT ACATTAGAGT ACAGAAGATT GGAGAAGGTT CATTCGGAAA AGCCATTCTT 
GTCAGATCTA CAGAAGATGG CAGACAATAT GTCATCAAAG AAATTAATAT CTCAAGGATG 
TCCAATAAAG AGAGGGAAGA ATCAAGGAGA GAAGTTGCAG TGCTAGCAAA CATGCGGCAT 
CCAAACATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATTGTAATG 
GATTACTGTG AAGGAGGGGA TCTGTTCAAA AGAATAAATG CTCAGAAGGG CGTTTTGTTT 
CAAGAGGACC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTAAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATAAAA TCACAGAACA TATTTTTAAC CAGAGATGGA 
ACAATACAAC TTGGAGATTT TGGAATCGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA TAAACCTTAT 
AATAATAAAA GTGATATTTG GGCTCTGGGG TGTGTCCTTT ATGAGATGTG CACACTTAAA 
CATGCATTTG AAGCTGGCAA TATGAAGAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCCGTGT CTTTGCATTA TTCTTACGAT CTCCGCAGTT TACTCTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC CCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCCAAGTTTG GAGCACAACC TCTACCAGCT AAAAGACCAG CTTCAGGACA AAGCTTGGCT 
TCTATTATGT CTGCTCAGAA AATTACAAAG CCTGCTGCTA AATATGGAAT ACCGTTAACA 
AATAAGAAAT ATATAGATAA AAAATTGCAT GAAAGGAAGC CACTCCAGAA ATATAAACAG 
GAAGCACCAA GAAAAAGAAG ATTGGAATTG ACTGAAAAAG AAAAGAAGTC TAAGGATCAG 
GTTAGTCTGC TGAAGGCTGA GCAGGTGAAA AGACAGGAAA AGCAAAGGTT GGAGAGAATA 
AATAGGGCCA GGGAACAAGG CTGGAGAAAT GTGCTGAGTG CTGGTGGAAG CGGTGAAATG 
AAGGCTCCCT TGTTGGGCAG TGGAGGGGCC ATGGCTCCAT CATCTTTTTC CTCTCGAGGA 
CAGTATGAAC ATTATCACGC TATTTTTGAT CAAATGCAGC AACAAAGAGC AGAAGATAAT 
GAAGCTAAAT GGAAAGGAGA AATACATGGC CAGAGGCTTC CAGAAAGGCA GAAGGGGCAG 
CCAGCTATGG AAAGAGCAAA ACAAGTAGAA GAGTTTCTAC AGCGAAGACG GGAAGCAAAG 
CAGAACAAAG CTCGGGCCGA GGGGCATATG GGCATCCTGC ACAACCTGGC AGCTCTCTAT 
GGAGGCAGGC CCAGCTCTTC ACGAGGAGGG AAGCCGAGAA ACAAAGAGGA AGAGGTTTAT 
TTGGCAAGAC TAAGACAAAT AAGACTGCAG AATTTCAATG AACGCCAACA GATTAGAGCC 
AAGCTTCGTG GTGAAAAGAA GGAAACTGAC AGCTCTGAAG ATCAAGAAGG AAGTGAGGAG 
GCTGATGTGA GGCGTAAAAA AGTGGAAGCC CTGAAGGCCC AGGCAAGTGC CCGTGCAGCC 
GTCCTGAAGG AGCAGCTTGA GCGCAAGCGG AAGGAGGCAT ATGAGAGAGA GAAGAAAGTG 
TGGGAGGAGC ATTTGGTGGC CAAGGGAATA AAGAACTCTG ATGTGTCTCC GCCGTCAGGG 
CAGCAGGAAA TGGGGGGTTC CCCAACAAAG CAGCCAATGA GACCTGTTAT TTCTGTGACT 
TCGGCTTTGA AGGAAGTGTG TATGGACCAA AGTCTAACTG ATGCCCAAGA AACCTCAGAA 
GAAATGCAGA AGAGTAACAG TGCTGTTTCA AGTAAACGAG AAATACTACG TAGATTAAAT 
CAAAATCTTA AAGTTCAAGA AAATGAAAAG GGAAGGCAGA GTCGTCGTGA AACGGGTACA 
GTAACTGTTC ATGAAGATGC CAAAGAGCTG GAAAAAGAAA AGTCAGTTGT ATCGGATCGA 
AAAAAATGGG AGACAGGAGG CCAGCTTGTG CTTCCTGTGG ACGAACTGGC CCTGGACGCT 
TCCTTCTCTG TGACAGAAAA ACATATAGTG GGAGAGGTTA TTAAATTAGA TCCTAGTGGA 
TCTCCAAGAA AAGTCTGGGG GAAAAGTCCT ACAGATTCTG TTCTGAGGAT ACTTGGAGAA 
GCTGAACTAC AACTTCAGAC AGAACTACTG GAAAACACAT CTATTAGAAA TGAGATTTCT 
CCTGAAAGCG AGAATTTCAA ATCCTTAATT ACTGAAGAAG AAAAAGTAAA ATGTATTCCA 
CTTGAAATGA ACCCACCAGC TACTCTTGAT TCTTCTGTTG AGACAAACAG TCCAAACATT 
AGTGAAGCAT CTCCACAGAC AGCATTGAAA GCAGAAGGAG ATTTGGAGGA GCCTGATGAC 
TTGGAGGCAG AAGTGCTGCA GGAGCCAAGT GGAGCAGACA GAGATGGTGG TTTGCCCCAA 
CCTGTTCTCG ACATGTGGGT CACTGAGAGA GAAGGAGCAG AGGAGGTTGA ATTGGAAGAT 
AGGATCACCA TTCAGCAAAA TGAACTTTCT GAAGATAAAG CCCCGAGCAG TGTGGATCAC 
CTTAGTGAGG TTCATGTAGA ACCTGGAGTG GATGATTCTC CACCCTCTAA GCGCGATATA 
GATAAATCTA TGCAACCAGA ACCATTTTTC CATAAAGTGG TTTATTCTGA GCATCTGAAA 
GCAGTTTCTC AGGTTCAGTC AGTTCTCTGT TCACCAGAAG AATCCTTTCC ACTTCGATCT 
CACTCTGATT CACCACCAAG GAATAAAAAA CAGAATTCCT TGCTGATTGG ACTTTCAACG 
GGTCTATTTG ATGCAAACAA CCCAAAGATG TTGCGGACAT GTTCACTTCC AGACCTCTCC 
AAACTGTTCA GAACACTGAT GGATGTCCCC ACTGTGGGAG ATGGACGTCA GGACAATCTT 
GAAATGGAAG ATGAGCATGT TAAGGAAGGG CCGTCGGACT CTGAGGACAT TGTGTTTGAA 
GAGGCTGACA CAGACTTACA GGAGCTCCAA GCCTCCATGG AGCAGCTGCT CCAGGAGCAG 
CCAGGGGAGG ACTACAGCGA AGAGGAGGAG TCGGGCTTGA AGACCAGTGA TGACGCCGAG 
CAGGCCGCCA ACGGCACGGA CGCGGGCGAG GAGGACGACA ACCCCAGCAG CGAGAGCGCC 
CTCAATGAGG AGTGGCACTC GGATAACAGT GATGGGGAGA TAACTAGTGA ATGTGAATAC 
GATAGTGTCT TTAATCATTT AGAGGAACTG AGGCTTCACC TGGAACAGGA AATAGGCTTC 
GAAAAGTTCT TTGAGGTTTA TGAAAAAATA AAGGCTGTTC ATGAAGATGA AGATGAAAAT 
ATTGAGATTT GTTCAACAAT AGTTCAGAAT ATTTTGGGAA ATGAACATCA GCATCTTTAT 
GCCAAGATTC TTCATTTAGT CATGGCAGAT GGAGCCTATC AAGAAGATAA TGATGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_015464415.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015319901.1 Bos taurus NIMA related kinase 1 (NEK1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa52911
Clone ID Related Accession (Same CDS sequence)	XM_015464415.1 , XM_010807677.2 , XM_010807677.1
Accession Version	XM_015464415.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3636bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	serine/threonine-protein kinase Nek1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645187.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAAT ACATTAGAGT ACAGAAGATT GGAGAAGGTT CATTCGGAAA AGCCATTCTT 
GTCAGATCTA CAGAAGATGG CAGACAATAT GTCATCAAAG AAATTAATAT CTCAAGGATG 
TCCAATAAAG AGAGGGAAGA ATCAAGGAGA GAAGTTGCAG TGCTAGCAAA CATGCGGCAT 
CCAAACATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATTGTAATG 
GATTACTGTG AAGGAGGGGA TCTGTTCAAA AGAATAAATG CTCAGAAGGG CGTTTTGTTT 
CAAGAGGACC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTAAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATAAAA TCACAGAACA TATTTTTAAC CAGAGATGGA 
ACAATACAAC TTGGAGATTT TGGAATCGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA TAAACCTTAT 
AATAATAAAA GTGATATTTG GGCTCTGGGG TGTGTCCTTT ATGAGATGTG CACACTTAAA 
CATGCATTTG AAGCTGGCAA TATGAAGAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCCGTGT CTTTGCATTA TTCTTACGAT CTCCGCAGTT TACTCTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC CCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCCAAGTTTG GAGCACAACC TCTACCAGCT AAAAGACCAG CTTCAGGACA AAGCTTGGCT 
TCTATTATGT CTGCTCAGAA AATTACAAAG CCTGCTGCTA AATATGGAAT ACCGTTAACA 
AATAAGAAAT ATATAGATAA AAAATTGCAT GAAAGGAAGC CACTCCAGAA ATATAAACAG 
GCCCATCAAA CCCCAGTGAA GAAAGTGAAT CCTGGAGAAG AAAGGAGGAA ACTGTTTGAG 
GAAGCACCAA GAAAAAGAAG ATTGGAATTG ACTGAAAAAG AAAAGAAGTC TAAGGATCAG 
GTTAGTCTGC TGAAGGCTGA GCAGGTGAAA AGACAGGAAA AGCAAAGGTT GGAGAGAATA 
AATAGGGCCA GGGAACAAGG CTGGAGAAAT GTGCTGAGTG CTGGTGGAAG CGGTGAAATG 
AAGGCTCCCT TGTTGGGCAG TGGAGGGGCC ATGGCTCCAT CATCTTTTTC CTCTCGAGGA 
CAGTATGAAC ATTATCACGC TATTTTTGAT CAAATGCAGC AACAAAGAGC AGAAGATAAT 
GAAGCTAAAT GGAAAGGAGA AATACATGGC CAGAGGCTTC CAGAAAGGCA GAAGGGGCAG 
CCAGCTATGG AAAGAGCAAA ACAAGTAGAA GAGTTTCTAC AGCGAAGACG GGAAGCAAAG 
CAGAACAAAG CTCGGGCCGA GGGGCATATG GTTTATTTGG CAAGACTAAG ACAAATAAGA 
CTGCAGAATT TCAATGAACG CCAACAGATT AGAGCCAAGC TTCGTGGTGA AAAGAAGGAA 
ACTGACAGCT CTGAAGATCA AGAAGGAAGT GAGGAGGCTG ATGTGAGGCG TAAAAAAGTG 
GAAGCCCTGA AGGCCCAGGC AAGTGCCCGT GCAGCCGTCC TGAAGGAGCA GCTTGAGCGC 
AAGCGGAAGG AGGCATATGA GAGAGAGAAG AAAGTGTGGG AGGAGCATTT GGTGGCCAAG 
GGAATAAAGA ACTCTGATGT GTCTCCGCCG TCAGGGCAGC AGGAAATGGG GGGTTCCCCA 
ACAAAGCAGC CAATGAGACC TGTTATTTCT GTGACTTCGG CTTTGAAGGA AGTGTGTATG 
GACCAAAGTC TAACTGATGC CCAAGAAACC TCAGAAGAAA TGCAGAAGAG TAACAGTGCT 
GTTTCAAGTA AACGAGAAAT ACTACGTAGA TTAAATCAAA ATCTTAAAGT TCAAGAAAAT 
GAAAAGGGAA GGCAGAGTCG TCGTGAAACG GGTACAGTAA CTGTTCATGA AGATGCCAAA 
GAGCTGGAAA AAGAAAAGTC AGTTGTATCG GATCGAAAAA AATGGGAGAC AGGAGGCCAG 
CTTGTGCTTC CTGTGGACGA ACTGGCCCTG GACGCTTCCT TCTCTGTGAC AGAAAAACAT 
ATAGTGGGAG AGGTTATTAA ATTAGATCCT AGTGGATCTC CAAGAAAAGT CTGGGGGAAA 
AGTCCTACAG ATTCTGTTCT GAGGATACTT GGAGAAGCTG AACTACAACT TCAGACAGAA 
CTACTGGAAA ACACATCTAT TAGAAATGAG ATTTCTCCTG AAAGCGAGAA TTTCAAATCC 
TTAATTACTG AAGAAGAAAA AGTAAAATGT ATTCCACTTG AAATGAACCC ACCAGCTACT 
CTTGATTCTT CTGTTGAGAC AAACAGTCCA AACATTAGTG AAGCATCTCC ACAGACAGCA 
TTGAAAGCAG AAGGAGATTT GGAGGAGCCT GATGACTTGG AGGCAGAAGT GCTGCAGGAG 
CCAAGTGGAG CAGACAGAGA TGGTGGTTTG CCCCAACCTG TTCTCGACAT GTGGGTCACT 
GAGAGAGAAG GAGCAGAGGA GGTTGAATTG GAAGATAGGA TCACCATTCA GCAAAATGAA 
CTTTCTGAAG ATAAAGCCCC GAGCAGTGTG GATCACCTTA GTGAGGTTCA TGTAGAACCT 
GGAGTGGATG ATTCTCCACC CTCTAAGCGC GATATAGATA AATCTATGCA ACCAGAACCA 
TTTTTCCATA AAGTGGTTTA TTCTGAGCAT CTGAAAGCAG TTTCTCAGGT TCAGTCAGTT 
CTCTGTTCAC CAGAAGAATC CTTTCCACTT CGATCTCACT CTGATTCACC ACCAAGGAAT 
AAAAAACAGA ATTCCTTGCT GATTGGACTT TCAACGGGTC TATTTGATGC AAACAACCCA 
AAGATGTTGC GGACATGTTC ACTTCCAGAC CTCTCCAAAC TGTTCAGAAC ACTGATGGAT 
GTCCCCACTG TGGGAGATGG ACGTCAGGAC AATCTTGAAA TGGAAGATGA GCATGTTAAG 
GAAGGGCCGT CGGACTCTGA GGACATTGTG TTTGAAGAGG CTGACACAGA CTTACAGGAG 
CTCCAAGCCT CCATGGAGCA GCTGCTCCAG GAGCAGCCAG GGGAGGACTA CAGCGAAGAG 
GAGGAGTCGG GCTTGAAGAC CAGTGATGAC GCCGAGCAGG CCGCCAACGG CACGGACGCG 
GGCGAGGAGG ACGACAACCC CAGCAGCGAG AGCGCCCTCA ATGAGGAGTG GCACTCGGAT 
AACAGTGATG GGGAGATAAC TAGTGAATGT GAATACGATA GTGTCTTTAA TCATTTAGAG 
GAACTGAGGC TTCACCTGGA ACAGGAAATA GGCTTCGAAA AGTTCTTTGA GGTTTATGAA 
AAAATAAAGG CTGTTCATGA AGATGAAGAT GAAAATATTG AGATTTGTTC AACAATAGTT 
CAGAATATTT TGGGAAATGA ACATCAGCAT CTTTATGCCA AGATTCTTCA TTTAGTCATG 
GCAGATGGAG CCTATCAAGA AGATAATGAT GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015319901.1
CDS	770..4405
Translation	MEKYIRVQKIGEGSFGKAILVRSTEDGRQYVIKEINISRMSNKEREESRREVAVLANMRHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGVLFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTRDGTIQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIISGSFPPVSLHYSYDLRSLLSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTFSKFGAQPLPAKRPASGQSLASIMSAQKITKPAAKYGIPLTNKKYIDKKLHERKPLQKYKQAHQTPVKKVNPGEERRKLFEEAPRKRRLELTEKEKKSKDQVSLLKAEQVKRQEKQRLERINRAREQGWRNVLSAGGSGEMKAPLLGSGGAMAPSSFSSRGQYEHYHAIFDQMQQQRAEDNEAKWKGEIHGQRLPERQKGQPAMERAKQVEEFLQRRREAKQNKARAEGHMVYLARLRQIRLQNFNERQQIRAKLRGEKKETDSSEDQEGSEEADVRRKKVEALKAQASARAAVLKEQLERKRKEAYEREKKVWEEHLVAKGIKNSDVSPPSGQQEMGGSPTKQPMRPVISVTSALKEVCMDQSLTDAQETSEEMQKSNSAVSSKREILRRLNQNLKVQENEKGRQSRRETGTVTVHEDAKELEKEKSVVSDRKKWETGGQLVLPVDELALDASFSVTEKHIVGEVIKLDPSGSPRKVWGKSPTDSVLRILGEAELQLQTELLENTSIRNEISPESENFKSLITEEEKVKCIPLEMNPPATLDSSVETNSPNISEASPQTALKAEGDLEEPDDLEAEVLQEPSGADRDGGLPQPVLDMWVTEREGAEEVELEDRITIQQNELSEDKAPSSVDHLSEVHVEPGVDDSPPSKRDIDKSMQPEPFFHKVVYSEHLKAVSQVQSVLCSPEESFPLRSHSDSPPRNKKQNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDGRQDNLEMEDEHVKEGPSDSEDIVFEEADTDLQELQASMEQLLQEQPGEDYSEEEESGLKTSDDAEQAANGTDAGEEDDNPSSESALNEEWHSDNSDGEITSECEYDSVFNHLEELRLHLEQEIGFEKFFEVYEKIKAVHEDEDENIEICSTIVQNILGNEHQHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_015464415.1
Organism	Bos taurus(cattle)
Definition	Bos taurus NIMA related kinase 1 (NEK1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015464415.1

ORF Insert Sequence:

ATGGAGAAAT ACATTAGAGT ACAGAAGATT GGAGAAGGTT CATTCGGAAA AGCCATTCTT 
GTCAGATCTA CAGAAGATGG CAGACAATAT GTCATCAAAG AAATTAATAT CTCAAGGATG 
TCCAATAAAG AGAGGGAAGA ATCAAGGAGA GAAGTTGCAG TGCTAGCAAA CATGCGGCAT 
CCAAACATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATTGTAATG 
GATTACTGTG AAGGAGGGGA TCTGTTCAAA AGAATAAATG CTCAGAAGGG CGTTTTGTTT 
CAAGAGGACC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTAAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATAAAA TCACAGAACA TATTTTTAAC CAGAGATGGA 
ACAATACAAC TTGGAGATTT TGGAATCGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA TAAACCTTAT 
AATAATAAAA GTGATATTTG GGCTCTGGGG TGTGTCCTTT ATGAGATGTG CACACTTAAA 
CATGCATTTG AAGCTGGCAA TATGAAGAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCCGTGT CTTTGCATTA TTCTTACGAT CTCCGCAGTT TACTCTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC CCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCCAAGTTTG GAGCACAACC TCTACCAGCT AAAAGACCAG CTTCAGGACA AAGCTTGGCT 
TCTATTATGT CTGCTCAGAA AATTACAAAG CCTGCTGCTA AATATGGAAT ACCGTTAACA 
AATAAGAAAT ATATAGATAA AAAATTGCAT GAAAGGAAGC CACTCCAGAA ATATAAACAG 
GCCCATCAAA CCCCAGTGAA GAAAGTGAAT CCTGGAGAAG AAAGGAGGAA ACTGTTTGAG 
GAAGCACCAA GAAAAAGAAG ATTGGAATTG ACTGAAAAAG AAAAGAAGTC TAAGGATCAG 
GTTAGTCTGC TGAAGGCTGA GCAGGTGAAA AGACAGGAAA AGCAAAGGTT GGAGAGAATA 
AATAGGGCCA GGGAACAAGG CTGGAGAAAT GTGCTGAGTG CTGGTGGAAG CGGTGAAATG 
AAGGCTCCCT TGTTGGGCAG TGGAGGGGCC ATGGCTCCAT CATCTTTTTC CTCTCGAGGA 
CAGTATGAAC ATTATCACGC TATTTTTGAT CAAATGCAGC AACAAAGAGC AGAAGATAAT 
GAAGCTAAAT GGAAAGGAGA AATACATGGC CAGAGGCTTC CAGAAAGGCA GAAGGGGCAG 
CCAGCTATGG AAAGAGCAAA ACAAGTAGAA GAGTTTCTAC AGCGAAGACG GGAAGCAAAG 
CAGAACAAAG CTCGGGCCGA GGGGCATATG GTTTATTTGG CAAGACTAAG ACAAATAAGA 
CTGCAGAATT TCAATGAACG CCAACAGATT AGAGCCAAGC TTCGTGGTGA AAAGAAGGAA 
ACTGACAGCT CTGAAGATCA AGAAGGAAGT GAGGAGGCTG ATGTGAGGCG TAAAAAAGTG 
GAAGCCCTGA AGGCCCAGGC AAGTGCCCGT GCAGCCGTCC TGAAGGAGCA GCTTGAGCGC 
AAGCGGAAGG AGGCATATGA GAGAGAGAAG AAAGTGTGGG AGGAGCATTT GGTGGCCAAG 
GGAATAAAGA ACTCTGATGT GTCTCCGCCG TCAGGGCAGC AGGAAATGGG GGGTTCCCCA 
ACAAAGCAGC CAATGAGACC TGTTATTTCT GTGACTTCGG CTTTGAAGGA AGTGTGTATG 
GACCAAAGTC TAACTGATGC CCAAGAAACC TCAGAAGAAA TGCAGAAGAG TAACAGTGCT 
GTTTCAAGTA AACGAGAAAT ACTACGTAGA TTAAATCAAA ATCTTAAAGT TCAAGAAAAT 
GAAAAGGGAA GGCAGAGTCG TCGTGAAACG GGTACAGTAA CTGTTCATGA AGATGCCAAA 
GAGCTGGAAA AAGAAAAGTC AGTTGTATCG GATCGAAAAA AATGGGAGAC AGGAGGCCAG 
CTTGTGCTTC CTGTGGACGA ACTGGCCCTG GACGCTTCCT TCTCTGTGAC AGAAAAACAT 
ATAGTGGGAG AGGTTATTAA ATTAGATCCT AGTGGATCTC CAAGAAAAGT CTGGGGGAAA 
AGTCCTACAG ATTCTGTTCT GAGGATACTT GGAGAAGCTG AACTACAACT TCAGACAGAA 
CTACTGGAAA ACACATCTAT TAGAAATGAG ATTTCTCCTG AAAGCGAGAA TTTCAAATCC 
TTAATTACTG AAGAAGAAAA AGTAAAATGT ATTCCACTTG AAATGAACCC ACCAGCTACT 
CTTGATTCTT CTGTTGAGAC AAACAGTCCA AACATTAGTG AAGCATCTCC ACAGACAGCA 
TTGAAAGCAG AAGGAGATTT GGAGGAGCCT GATGACTTGG AGGCAGAAGT GCTGCAGGAG 
CCAAGTGGAG CAGACAGAGA TGGTGGTTTG CCCCAACCTG TTCTCGACAT GTGGGTCACT 
GAGAGAGAAG GAGCAGAGGA GGTTGAATTG GAAGATAGGA TCACCATTCA GCAAAATGAA 
CTTTCTGAAG ATAAAGCCCC GAGCAGTGTG GATCACCTTA GTGAGGTTCA TGTAGAACCT 
GGAGTGGATG ATTCTCCACC CTCTAAGCGC GATATAGATA AATCTATGCA ACCAGAACCA 
TTTTTCCATA AAGTGGTTTA TTCTGAGCAT CTGAAAGCAG TTTCTCAGGT TCAGTCAGTT 
CTCTGTTCAC CAGAAGAATC CTTTCCACTT CGATCTCACT CTGATTCACC ACCAAGGAAT 
AAAAAACAGA ATTCCTTGCT GATTGGACTT TCAACGGGTC TATTTGATGC AAACAACCCA 
AAGATGTTGC GGACATGTTC ACTTCCAGAC CTCTCCAAAC TGTTCAGAAC ACTGATGGAT 
GTCCCCACTG TGGGAGATGG ACGTCAGGAC AATCTTGAAA TGGAAGATGA GCATGTTAAG 
GAAGGGCCGT CGGACTCTGA GGACATTGTG TTTGAAGAGG CTGACACAGA CTTACAGGAG 
CTCCAAGCCT CCATGGAGCA GCTGCTCCAG GAGCAGCCAG GGGAGGACTA CAGCGAAGAG 
GAGGAGTCGG GCTTGAAGAC CAGTGATGAC GCCGAGCAGG CCGCCAACGG CACGGACGCG 
GGCGAGGAGG ACGACAACCC CAGCAGCGAG AGCGCCCTCA ATGAGGAGTG GCACTCGGAT 
AACAGTGATG GGGAGATAAC TAGTGAATGT GAATACGATA GTGTCTTTAA TCATTTAGAG 
GAACTGAGGC TTCACCTGGA ACAGGAAATA GGCTTCGAAA AGTTCTTTGA GGTTTATGAA 
AAAATAAAGG CTGTTCATGA AGATGAAGAT GAAAATATTG AGATTTGTTC AACAATAGTT 
CAGAATATTT TGGGAAATGA ACATCAGCAT CTTTATGCCA AGATTCTTCA TTTAGTCATG 
GCAGATGGAG CCTATCAAGA AGATAATGAT GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_010807677.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010805979.1 Bos taurus NIMA related kinase 1 (NEK1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa52911
Clone ID Related Accession (Same CDS sequence)	XM_015464415.1 , XM_010807677.2 , XM_010807677.1
Accession Version	XM_010807677.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3636bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	serine/threonine-protein kinase Nek1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010807677.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAAT ACATTAGAGT ACAGAAGATT GGAGAAGGTT CATTCGGAAA AGCCATTCTT 
GTCAGATCTA CAGAAGATGG CAGACAATAT GTCATCAAAG AAATTAATAT CTCAAGGATG 
TCCAATAAAG AGAGGGAAGA ATCAAGGAGA GAAGTTGCAG TGCTAGCAAA CATGCGGCAT 
CCAAACATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATTGTAATG 
GATTACTGTG AAGGAGGGGA TCTGTTCAAA AGAATAAATG CTCAGAAGGG CGTTTTGTTT 
CAAGAGGACC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTAAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATAAAA TCACAGAACA TATTTTTAAC CAGAGATGGA 
ACAATACAAC TTGGAGATTT TGGAATCGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA TAAACCTTAT 
AATAATAAAA GTGATATTTG GGCTCTGGGG TGTGTCCTTT ATGAGATGTG CACACTTAAA 
CATGCATTTG AAGCTGGCAA TATGAAGAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCCGTGT CTTTGCATTA TTCTTACGAT CTCCGCAGTT TACTCTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC CCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCCAAGTTTG GAGCACAACC TCTACCAGCT AAAAGACCAG CTTCAGGACA AAGCTTGGCT 
TCTATTATGT CTGCTCAGAA AATTACAAAG CCTGCTGCTA AATATGGAAT ACCGTTAACA 
AATAAGAAAT ATATAGATAA AAAATTGCAT GAAAGGAAGC CACTCCAGAA ATATAAACAG 
GCCCATCAAA CCCCAGTGAA GAAAGTGAAT CCTGGAGAAG AAAGGAGGAA ACTGTTTGAG 
GAAGCACCAA GAAAAAGAAG ATTGGAATTG ACTGAAAAAG AAAAGAAGTC TAAGGATCAG 
GTTAGTCTGC TGAAGGCTGA GCAGGTGAAA AGACAGGAAA AGCAAAGGTT GGAGAGAATA 
AATAGGGCCA GGGAACAAGG CTGGAGAAAT GTGCTGAGTG CTGGTGGAAG CGGTGAAATG 
AAGGCTCCCT TGTTGGGCAG TGGAGGGGCC ATGGCTCCAT CATCTTTTTC CTCTCGAGGA 
CAGTATGAAC ATTATCACGC TATTTTTGAT CAAATGCAGC AACAAAGAGC AGAAGATAAT 
GAAGCTAAAT GGAAAGGAGA AATACATGGC CAGAGGCTTC CAGAAAGGCA GAAGGGGCAG 
CCAGCTATGG AAAGAGCAAA ACAAGTAGAA GAGTTTCTAC AGCGAAGACG GGAAGCAAAG 
CAGAACAAAG CTCGGGCCGA GGGGCATATG GTTTATTTGG CAAGACTAAG ACAAATAAGA 
CTGCAGAATT TCAATGAACG CCAACAGATT AGAGCCAAGC TTCGTGGTGA AAAGAAGGAA 
ACTGACAGCT CTGAAGATCA AGAAGGAAGT GAGGAGGCTG ATGTGAGGCG TAAAAAAGTG 
GAAGCCCTGA AGGCCCAGGC AAGTGCCCGT GCAGCCGTCC TGAAGGAGCA GCTTGAGCGC 
AAGCGGAAGG AGGCATATGA GAGAGAGAAG AAAGTGTGGG AGGAGCATTT GGTGGCCAAG 
GGAATAAAGA ACTCTGATGT GTCTCCGCCG TCAGGGCAGC AGGAAATGGG GGGTTCCCCA 
ACAAAGCAGC CAATGAGACC TGTTATTTCT GTGACTTCGG CTTTGAAGGA AGTGTGTATG 
GACCAAAGTC TAACTGATGC CCAAGAAACC TCAGAAGAAA TGCAGAAGAG TAACAGTGCT 
GTTTCAAGTA AACGAGAAAT ACTACGTAGA TTAAATCAAA ATCTTAAAGT TCAAGAAAAT 
GAAAAGGGAA GGCAGAGTCG TCGTGAAACG GGTACAGTAA CTGTTCATGA AGATGCCAAA 
GAGCTGGAAA AAGAAAAGTC AGTTGTATCG GATCGAAAAA AATGGGAGAC AGGAGGCCAG 
CTTGTGCTTC CTGTGGACGA ACTGGCCCTG GACGCTTCCT TCTCTGTGAC AGAAAAACAT 
ATAGTGGGAG AGGTTATTAA ATTAGATCCT AGTGGATCTC CAAGAAAAGT CTGGGGGAAA 
AGTCCTACAG ATTCTGTTCT GAGGATACTT GGAGAAGCTG AACTACAACT TCAGACAGAA 
CTACTGGAAA ACACATCTAT TAGAAATGAG ATTTCTCCTG AAAGCGAGAA TTTCAAATCC 
TTAATTACTG AAGAAGAAAA AGTAAAATGT ATTCCACTTG AAATGAACCC ACCAGCTACT 
CTTGATTCTT CTGTTGAGAC AAACAGTCCA AACATTAGTG AAGCATCTCC ACAGACAGCA 
TTGAAAGCAG AAGGAGATTT GGAGGAGCCT GATGACTTGG AGGCAGAAGT GCTGCAGGAG 
CCAAGTGGAG CAGACAGAGA TGGTGGTTTG CCCCAACCTG TTCTCGACAT GTGGGTCACT 
GAGAGAGAAG GAGCAGAGGA GGTTGAATTG GAAGATAGGA TCACCATTCA GCAAAATGAA 
CTTTCTGAAG ATAAAGCCCC GAGCAGTGTG GATCACCTTA GTGAGGTTCA TGTAGAACCT 
GGAGTGGATG ATTCTCCACC CTCTAAGCGC GATATAGATA AATCTATGCA ACCAGAACCA 
TTTTTCCATA AAGTGGTTTA TTCTGAGCAT CTGAAAGCAG TTTCTCAGGT TCAGTCAGTT 
CTCTGTTCAC CAGAAGAATC CTTTCCACTT CGATCTCACT CTGATTCACC ACCAAGGAAT 
AAAAAACAGA ATTCCTTGCT GATTGGACTT TCAACGGGTC TATTTGATGC AAACAACCCA 
AAGATGTTGC GGACATGTTC ACTTCCAGAC CTCTCCAAAC TGTTCAGAAC ACTGATGGAT 
GTCCCCACTG TGGGAGATGG ACGTCAGGAC AATCTTGAAA TGGAAGATGA GCATGTTAAG 
GAAGGGCCGT CGGACTCTGA GGACATTGTG TTTGAAGAGG CTGACACAGA CTTACAGGAG 
CTCCAAGCCT CCATGGAGCA GCTGCTCCAG GAGCAGCCAG GGGAGGACTA CAGCGAAGAG 
GAGGAGTCGG GCTTGAAGAC CAGTGATGAC GCCGAGCAGG CCGCCAACGG CACGGACGCG 
GGCGAGGAGG ACGACAACCC CAGCAGCGAG AGCGCCCTCA ATGAGGAGTG GCACTCGGAT 
AACAGTGATG GGGAGATAAC TAGTGAATGT GAATACGATA GTGTCTTTAA TCATTTAGAG 
GAACTGAGGC TTCACCTGGA ACAGGAAATA GGCTTCGAAA AGTTCTTTGA GGTTTATGAA 
AAAATAAAGG CTGTTCATGA AGATGAAGAT GAAAATATTG AGATTTGTTC AACAATAGTT 
CAGAATATTT TGGGAAATGA ACATCAGCAT CTTTATGCCA AGATTCTTCA TTTAGTCATG 
GCAGATGGAG CCTATCAAGA AGATAATGAT GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010805979.1
CDS	1123..4758
Translation	MEKYIRVQKIGEGSFGKAILVRSTEDGRQYVIKEINISRMSNKEREESRREVAVLANMRHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGVLFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTRDGTIQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIISGSFPPVSLHYSYDLRSLLSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTFSKFGAQPLPAKRPASGQSLASIMSAQKITKPAAKYGIPLTNKKYIDKKLHERKPLQKYKQAHQTPVKKVNPGEERRKLFEEAPRKRRLELTEKEKKSKDQVSLLKAEQVKRQEKQRLERINRAREQGWRNVLSAGGSGEMKAPLLGSGGAMAPSSFSSRGQYEHYHAIFDQMQQQRAEDNEAKWKGEIHGQRLPERQKGQPAMERAKQVEEFLQRRREAKQNKARAEGHMVYLARLRQIRLQNFNERQQIRAKLRGEKKETDSSEDQEGSEEADVRRKKVEALKAQASARAAVLKEQLERKRKEAYEREKKVWEEHLVAKGIKNSDVSPPSGQQEMGGSPTKQPMRPVISVTSALKEVCMDQSLTDAQETSEEMQKSNSAVSSKREILRRLNQNLKVQENEKGRQSRRETGTVTVHEDAKELEKEKSVVSDRKKWETGGQLVLPVDELALDASFSVTEKHIVGEVIKLDPSGSPRKVWGKSPTDSVLRILGEAELQLQTELLENTSIRNEISPESENFKSLITEEEKVKCIPLEMNPPATLDSSVETNSPNISEASPQTALKAEGDLEEPDDLEAEVLQEPSGADRDGGLPQPVLDMWVTEREGAEEVELEDRITIQQNELSEDKAPSSVDHLSEVHVEPGVDDSPPSKRDIDKSMQPEPFFHKVVYSEHLKAVSQVQSVLCSPEESFPLRSHSDSPPRNKKQNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDGRQDNLEMEDEHVKEGPSDSEDIVFEEADTDLQELQASMEQLLQEQPGEDYSEEEESGLKTSDDAEQAANGTDAGEEDDNPSSESALNEEWHSDNSDGEITSECEYDSVFNHLEELRLHLEQEIGFEKFFEVYEKIKAVHEDEDENIEICSTIVQNILGNEHQHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_010807677.2
Organism	Bos taurus(cattle)
Definition	Bos taurus NIMA related kinase 1 (NEK1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010807677.2

ORF Insert Sequence:

ATGGAGAAAT ACATTAGAGT ACAGAAGATT GGAGAAGGTT CATTCGGAAA AGCCATTCTT 
GTCAGATCTA CAGAAGATGG CAGACAATAT GTCATCAAAG AAATTAATAT CTCAAGGATG 
TCCAATAAAG AGAGGGAAGA ATCAAGGAGA GAAGTTGCAG TGCTAGCAAA CATGCGGCAT 
CCAAACATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATTGTAATG 
GATTACTGTG AAGGAGGGGA TCTGTTCAAA AGAATAAATG CTCAGAAGGG CGTTTTGTTT 
CAAGAGGACC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTAAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATAAAA TCACAGAACA TATTTTTAAC CAGAGATGGA 
ACAATACAAC TTGGAGATTT TGGAATCGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA TAAACCTTAT 
AATAATAAAA GTGATATTTG GGCTCTGGGG TGTGTCCTTT ATGAGATGTG CACACTTAAA 
CATGCATTTG AAGCTGGCAA TATGAAGAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCCGTGT CTTTGCATTA TTCTTACGAT CTCCGCAGTT TACTCTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC CCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCCAAGTTTG GAGCACAACC TCTACCAGCT AAAAGACCAG CTTCAGGACA AAGCTTGGCT 
TCTATTATGT CTGCTCAGAA AATTACAAAG CCTGCTGCTA AATATGGAAT ACCGTTAACA 
AATAAGAAAT ATATAGATAA AAAATTGCAT GAAAGGAAGC CACTCCAGAA ATATAAACAG 
GCCCATCAAA CCCCAGTGAA GAAAGTGAAT CCTGGAGAAG AAAGGAGGAA ACTGTTTGAG 
GAAGCACCAA GAAAAAGAAG ATTGGAATTG ACTGAAAAAG AAAAGAAGTC TAAGGATCAG 
GTTAGTCTGC TGAAGGCTGA GCAGGTGAAA AGACAGGAAA AGCAAAGGTT GGAGAGAATA 
AATAGGGCCA GGGAACAAGG CTGGAGAAAT GTGCTGAGTG CTGGTGGAAG CGGTGAAATG 
AAGGCTCCCT TGTTGGGCAG TGGAGGGGCC ATGGCTCCAT CATCTTTTTC CTCTCGAGGA 
CAGTATGAAC ATTATCACGC TATTTTTGAT CAAATGCAGC AACAAAGAGC AGAAGATAAT 
GAAGCTAAAT GGAAAGGAGA AATACATGGC CAGAGGCTTC CAGAAAGGCA GAAGGGGCAG 
CCAGCTATGG AAAGAGCAAA ACAAGTAGAA GAGTTTCTAC AGCGAAGACG GGAAGCAAAG 
CAGAACAAAG CTCGGGCCGA GGGGCATATG GTTTATTTGG CAAGACTAAG ACAAATAAGA 
CTGCAGAATT TCAATGAACG CCAACAGATT AGAGCCAAGC TTCGTGGTGA AAAGAAGGAA 
ACTGACAGCT CTGAAGATCA AGAAGGAAGT GAGGAGGCTG ATGTGAGGCG TAAAAAAGTG 
GAAGCCCTGA AGGCCCAGGC AAGTGCCCGT GCAGCCGTCC TGAAGGAGCA GCTTGAGCGC 
AAGCGGAAGG AGGCATATGA GAGAGAGAAG AAAGTGTGGG AGGAGCATTT GGTGGCCAAG 
GGAATAAAGA ACTCTGATGT GTCTCCGCCG TCAGGGCAGC AGGAAATGGG GGGTTCCCCA 
ACAAAGCAGC CAATGAGACC TGTTATTTCT GTGACTTCGG CTTTGAAGGA AGTGTGTATG 
GACCAAAGTC TAACTGATGC CCAAGAAACC TCAGAAGAAA TGCAGAAGAG TAACAGTGCT 
GTTTCAAGTA AACGAGAAAT ACTACGTAGA TTAAATCAAA ATCTTAAAGT TCAAGAAAAT 
GAAAAGGGAA GGCAGAGTCG TCGTGAAACG GGTACAGTAA CTGTTCATGA AGATGCCAAA 
GAGCTGGAAA AAGAAAAGTC AGTTGTATCG GATCGAAAAA AATGGGAGAC AGGAGGCCAG 
CTTGTGCTTC CTGTGGACGA ACTGGCCCTG GACGCTTCCT TCTCTGTGAC AGAAAAACAT 
ATAGTGGGAG AGGTTATTAA ATTAGATCCT AGTGGATCTC CAAGAAAAGT CTGGGGGAAA 
AGTCCTACAG ATTCTGTTCT GAGGATACTT GGAGAAGCTG AACTACAACT TCAGACAGAA 
CTACTGGAAA ACACATCTAT TAGAAATGAG ATTTCTCCTG AAAGCGAGAA TTTCAAATCC 
TTAATTACTG AAGAAGAAAA AGTAAAATGT ATTCCACTTG AAATGAACCC ACCAGCTACT 
CTTGATTCTT CTGTTGAGAC AAACAGTCCA AACATTAGTG AAGCATCTCC ACAGACAGCA 
TTGAAAGCAG AAGGAGATTT GGAGGAGCCT GATGACTTGG AGGCAGAAGT GCTGCAGGAG 
CCAAGTGGAG CAGACAGAGA TGGTGGTTTG CCCCAACCTG TTCTCGACAT GTGGGTCACT 
GAGAGAGAAG GAGCAGAGGA GGTTGAATTG GAAGATAGGA TCACCATTCA GCAAAATGAA 
CTTTCTGAAG ATAAAGCCCC GAGCAGTGTG GATCACCTTA GTGAGGTTCA TGTAGAACCT 
GGAGTGGATG ATTCTCCACC CTCTAAGCGC GATATAGATA AATCTATGCA ACCAGAACCA 
TTTTTCCATA AAGTGGTTTA TTCTGAGCAT CTGAAAGCAG TTTCTCAGGT TCAGTCAGTT 
CTCTGTTCAC CAGAAGAATC CTTTCCACTT CGATCTCACT CTGATTCACC ACCAAGGAAT 
AAAAAACAGA ATTCCTTGCT GATTGGACTT TCAACGGGTC TATTTGATGC AAACAACCCA 
AAGATGTTGC GGACATGTTC ACTTCCAGAC CTCTCCAAAC TGTTCAGAAC ACTGATGGAT 
GTCCCCACTG TGGGAGATGG ACGTCAGGAC AATCTTGAAA TGGAAGATGA GCATGTTAAG 
GAAGGGCCGT CGGACTCTGA GGACATTGTG TTTGAAGAGG CTGACACAGA CTTACAGGAG 
CTCCAAGCCT CCATGGAGCA GCTGCTCCAG GAGCAGCCAG GGGAGGACTA CAGCGAAGAG 
GAGGAGTCGG GCTTGAAGAC CAGTGATGAC GCCGAGCAGG CCGCCAACGG CACGGACGCG 
GGCGAGGAGG ACGACAACCC CAGCAGCGAG AGCGCCCTCA ATGAGGAGTG GCACTCGGAT 
AACAGTGATG GGGAGATAAC TAGTGAATGT GAATACGATA GTGTCTTTAA TCATTTAGAG 
GAACTGAGGC TTCACCTGGA ACAGGAAATA GGCTTCGAAA AGTTCTTTGA GGTTTATGAA 
AAAATAAAGG CTGTTCATGA AGATGAAGAT GAAAATATTG AGATTTGTTC AACAATAGTT 
CAGAATATTT TGGGAAATGA ACATCAGCAT CTTTATGCCA AGATTCTTCA TTTAGTCATG 
GCAGATGGAG CCTATCAAGA AGATAATGAT GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_010807677.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010805979.1 Bos taurus NIMA related kinase 1 (NEK1), transcript variant X3, mRNA.

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CloneID	OBa52911
Clone ID Related Accession (Same CDS sequence)	XM_015464415.1 , XM_010807677.2 , XM_010807677.1
Accession Version	XM_010807677.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3636bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	serine/threonine-protein kinase Nek1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104069.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAAT ACATTAGAGT ACAGAAGATT GGAGAAGGTT CATTCGGAAA AGCCATTCTT 
GTCAGATCTA CAGAAGATGG CAGACAATAT GTCATCAAAG AAATTAATAT CTCAAGGATG 
TCCAATAAAG AGAGGGAAGA ATCAAGGAGA GAAGTTGCAG TGCTAGCAAA CATGCGGCAT 
CCAAACATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATTGTAATG 
GATTACTGTG AAGGAGGGGA TCTGTTCAAA AGAATAAATG CTCAGAAGGG CGTTTTGTTT 
CAAGAGGACC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTAAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATAAAA TCACAGAACA TATTTTTAAC CAGAGATGGA 
ACAATACAAC TTGGAGATTT TGGAATCGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA TAAACCTTAT 
AATAATAAAA GTGATATTTG GGCTCTGGGG TGTGTCCTTT ATGAGATGTG CACACTTAAA 
CATGCATTTG AAGCTGGCAA TATGAAGAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCCGTGT CTTTGCATTA TTCTTACGAT CTCCGCAGTT TACTCTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC CCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCCAAGTTTG GAGCACAACC TCTACCAGCT AAAAGACCAG CTTCAGGACA AAGCTTGGCT 
TCTATTATGT CTGCTCAGAA AATTACAAAG CCTGCTGCTA AATATGGAAT ACCGTTAACA 
AATAAGAAAT ATATAGATAA AAAATTGCAT GAAAGGAAGC CACTCCAGAA ATATAAACAG 
GCCCATCAAA CCCCAGTGAA GAAAGTGAAT CCTGGAGAAG AAAGGAGGAA ACTGTTTGAG 
GAAGCACCAA GAAAAAGAAG ATTGGAATTG ACTGAAAAAG AAAAGAAGTC TAAGGATCAG 
GTTAGTCTGC TGAAGGCTGA GCAGGTGAAA AGACAGGAAA AGCAAAGGTT GGAGAGAATA 
AATAGGGCCA GGGAACAAGG CTGGAGAAAT GTGCTGAGTG CTGGTGGAAG CGGTGAAATG 
AAGGCTCCCT TGTTGGGCAG TGGAGGGGCC ATGGCTCCAT CATCTTTTTC CTCTCGAGGA 
CAGTATGAAC ATTATCACGC TATTTTTGAT CAAATGCAGC AACAAAGAGC AGAAGATAAT 
GAAGCTAAAT GGAAAGGAGA AATACATGGC CAGAGGCTTC CAGAAAGGCA GAAGGGGCAG 
CCAGCTATGG AAAGAGCAAA ACAAGTAGAA GAGTTTCTAC AGCGAAGACG GGAAGCAAAG 
CAGAACAAAG CTCGGGCCGA GGGGCATATG GTTTATTTGG CAAGACTAAG ACAAATAAGA 
CTGCAGAATT TCAATGAACG CCAACAGATT AGAGCCAAGC TTCGTGGTGA AAAGAAGGAA 
ACTGACAGCT CTGAAGATCA AGAAGGAAGT GAGGAGGCTG ATGTGAGGCG TAAAAAAGTG 
GAAGCCCTGA AGGCCCAGGC AAGTGCCCGT GCAGCCGTCC TGAAGGAGCA GCTTGAGCGC 
AAGCGGAAGG AGGCATATGA GAGAGAGAAG AAAGTGTGGG AGGAGCATTT GGTGGCCAAG 
GGAATAAAGA ACTCTGATGT GTCTCCGCCG TCAGGGCAGC AGGAAATGGG GGGTTCCCCA 
ACAAAGCAGC CAATGAGACC TGTTATTTCT GTGACTTCGG CTTTGAAGGA AGTGTGTATG 
GACCAAAGTC TAACTGATGC CCAAGAAACC TCAGAAGAAA TGCAGAAGAG TAACAGTGCT 
GTTTCAAGTA AACGAGAAAT ACTACGTAGA TTAAATCAAA ATCTTAAAGT TCAAGAAAAT 
GAAAAGGGAA GGCAGAGTCG TCGTGAAACG GGTACAGTAA CTGTTCATGA AGATGCCAAA 
GAGCTGGAAA AAGAAAAGTC AGTTGTATCG GATCGAAAAA AATGGGAGAC AGGAGGCCAG 
CTTGTGCTTC CTGTGGACGA ACTGGCCCTG GACGCTTCCT TCTCTGTGAC AGAAAAACAT 
ATAGTGGGAG AGGTTATTAA ATTAGATCCT AGTGGATCTC CAAGAAAAGT CTGGGGGAAA 
AGTCCTACAG ATTCTGTTCT GAGGATACTT GGAGAAGCTG AACTACAACT TCAGACAGAA 
CTACTGGAAA ACACATCTAT TAGAAATGAG ATTTCTCCTG AAAGCGAGAA TTTCAAATCC 
TTAATTACTG AAGAAGAAAA AGTAAAATGT ATTCCACTTG AAATGAACCC ACCAGCTACT 
CTTGATTCTT CTGTTGAGAC AAACAGTCCA AACATTAGTG AAGCATCTCC ACAGACAGCA 
TTGAAAGCAG AAGGAGATTT GGAGGAGCCT GATGACTTGG AGGCAGAAGT GCTGCAGGAG 
CCAAGTGGAG CAGACAGAGA TGGTGGTTTG CCCCAACCTG TTCTCGACAT GTGGGTCACT 
GAGAGAGAAG GAGCAGAGGA GGTTGAATTG GAAGATAGGA TCACCATTCA GCAAAATGAA 
CTTTCTGAAG ATAAAGCCCC GAGCAGTGTG GATCACCTTA GTGAGGTTCA TGTAGAACCT 
GGAGTGGATG ATTCTCCACC CTCTAAGCGC GATATAGATA AATCTATGCA ACCAGAACCA 
TTTTTCCATA AAGTGGTTTA TTCTGAGCAT CTGAAAGCAG TTTCTCAGGT TCAGTCAGTT 
CTCTGTTCAC CAGAAGAATC CTTTCCACTT CGATCTCACT CTGATTCACC ACCAAGGAAT 
AAAAAACAGA ATTCCTTGCT GATTGGACTT TCAACGGGTC TATTTGATGC AAACAACCCA 
AAGATGTTGC GGACATGTTC ACTTCCAGAC CTCTCCAAAC TGTTCAGAAC ACTGATGGAT 
GTCCCCACTG TGGGAGATGG ACGTCAGGAC AATCTTGAAA TGGAAGATGA GCATGTTAAG 
GAAGGGCCGT CGGACTCTGA GGACATTGTG TTTGAAGAGG CTGACACAGA CTTACAGGAG 
CTCCAAGCCT CCATGGAGCA GCTGCTCCAG GAGCAGCCAG GGGAGGACTA CAGCGAAGAG 
GAGGAGTCGG GCTTGAAGAC CAGTGATGAC GCCGAGCAGG CCGCCAACGG CACGGACGCG 
GGCGAGGAGG ACGACAACCC CAGCAGCGAG AGCGCCCTCA ATGAGGAGTG GCACTCGGAT 
AACAGTGATG GGGAGATAAC TAGTGAATGT GAATACGATA GTGTCTTTAA TCATTTAGAG 
GAACTGAGGC TTCACCTGGA ACAGGAAATA GGCTTCGAAA AGTTCTTTGA GGTTTATGAA 
AAAATAAAGG CTGTTCATGA AGATGAAGAT GAAAATATTG AGATTTGTTC AACAATAGTT 
CAGAATATTT TGGGAAATGA ACATCAGCAT CTTTATGCCA AGATTCTTCA TTTAGTCATG 
GCAGATGGAG CCTATCAAGA AGATAATGAT GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010805979.1
CDS	770..4405
Translation	MEKYIRVQKIGEGSFGKAILVRSTEDGRQYVIKEINISRMSNKEREESRREVAVLANMRHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGVLFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTRDGTIQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIISGSFPPVSLHYSYDLRSLLSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTFSKFGAQPLPAKRPASGQSLASIMSAQKITKPAAKYGIPLTNKKYIDKKLHERKPLQKYKQAHQTPVKKVNPGEERRKLFEEAPRKRRLELTEKEKKSKDQVSLLKAEQVKRQEKQRLERINRAREQGWRNVLSAGGSGEMKAPLLGSGGAMAPSSFSSRGQYEHYHAIFDQMQQQRAEDNEAKWKGEIHGQRLPERQKGQPAMERAKQVEEFLQRRREAKQNKARAEGHMVYLARLRQIRLQNFNERQQIRAKLRGEKKETDSSEDQEGSEEADVRRKKVEALKAQASARAAVLKEQLERKRKEAYEREKKVWEEHLVAKGIKNSDVSPPSGQQEMGGSPTKQPMRPVISVTSALKEVCMDQSLTDAQETSEEMQKSNSAVSSKREILRRLNQNLKVQENEKGRQSRRETGTVTVHEDAKELEKEKSVVSDRKKWETGGQLVLPVDELALDASFSVTEKHIVGEVIKLDPSGSPRKVWGKSPTDSVLRILGEAELQLQTELLENTSIRNEISPESENFKSLITEEEKVKCIPLEMNPPATLDSSVETNSPNISEASPQTALKAEGDLEEPDDLEAEVLQEPSGADRDGGLPQPVLDMWVTEREGAEEVELEDRITIQQNELSEDKAPSSVDHLSEVHVEPGVDDSPPSKRDIDKSMQPEPFFHKVVYSEHLKAVSQVQSVLCSPEESFPLRSHSDSPPRNKKQNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDGRQDNLEMEDEHVKEGPSDSEDIVFEEADTDLQELQASMEQLLQEQPGEDYSEEEESGLKTSDDAEQAANGTDAGEEDDNPSSESALNEEWHSDNSDGEITSECEYDSVFNHLEELRLHLEQEIGFEKFFEVYEKIKAVHEDEDENIEICSTIVQNILGNEHQHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_010807677.1
Organism	Bos taurus(cattle)
Definition	Bos taurus NIMA related kinase 1 (NEK1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010807677.1

ORF Insert Sequence:

ATGGAGAAAT ACATTAGAGT ACAGAAGATT GGAGAAGGTT CATTCGGAAA AGCCATTCTT 
GTCAGATCTA CAGAAGATGG CAGACAATAT GTCATCAAAG AAATTAATAT CTCAAGGATG 
TCCAATAAAG AGAGGGAAGA ATCAAGGAGA GAAGTTGCAG TGCTAGCAAA CATGCGGCAT 
CCAAACATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATTGTAATG 
GATTACTGTG AAGGAGGGGA TCTGTTCAAA AGAATAAATG CTCAGAAGGG CGTTTTGTTT 
CAAGAGGACC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTAAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATAAAA TCACAGAACA TATTTTTAAC CAGAGATGGA 
ACAATACAAC TTGGAGATTT TGGAATCGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA TAAACCTTAT 
AATAATAAAA GTGATATTTG GGCTCTGGGG TGTGTCCTTT ATGAGATGTG CACACTTAAA 
CATGCATTTG AAGCTGGCAA TATGAAGAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCCGTGT CTTTGCATTA TTCTTACGAT CTCCGCAGTT TACTCTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC CCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCCAAGTTTG GAGCACAACC TCTACCAGCT AAAAGACCAG CTTCAGGACA AAGCTTGGCT 
TCTATTATGT CTGCTCAGAA AATTACAAAG CCTGCTGCTA AATATGGAAT ACCGTTAACA 
AATAAGAAAT ATATAGATAA AAAATTGCAT GAAAGGAAGC CACTCCAGAA ATATAAACAG 
GCCCATCAAA CCCCAGTGAA GAAAGTGAAT CCTGGAGAAG AAAGGAGGAA ACTGTTTGAG 
GAAGCACCAA GAAAAAGAAG ATTGGAATTG ACTGAAAAAG AAAAGAAGTC TAAGGATCAG 
GTTAGTCTGC TGAAGGCTGA GCAGGTGAAA AGACAGGAAA AGCAAAGGTT GGAGAGAATA 
AATAGGGCCA GGGAACAAGG CTGGAGAAAT GTGCTGAGTG CTGGTGGAAG CGGTGAAATG 
AAGGCTCCCT TGTTGGGCAG TGGAGGGGCC ATGGCTCCAT CATCTTTTTC CTCTCGAGGA 
CAGTATGAAC ATTATCACGC TATTTTTGAT CAAATGCAGC AACAAAGAGC AGAAGATAAT 
GAAGCTAAAT GGAAAGGAGA AATACATGGC CAGAGGCTTC CAGAAAGGCA GAAGGGGCAG 
CCAGCTATGG AAAGAGCAAA ACAAGTAGAA GAGTTTCTAC AGCGAAGACG GGAAGCAAAG 
CAGAACAAAG CTCGGGCCGA GGGGCATATG GTTTATTTGG CAAGACTAAG ACAAATAAGA 
CTGCAGAATT TCAATGAACG CCAACAGATT AGAGCCAAGC TTCGTGGTGA AAAGAAGGAA 
ACTGACAGCT CTGAAGATCA AGAAGGAAGT GAGGAGGCTG ATGTGAGGCG TAAAAAAGTG 
GAAGCCCTGA AGGCCCAGGC AAGTGCCCGT GCAGCCGTCC TGAAGGAGCA GCTTGAGCGC 
AAGCGGAAGG AGGCATATGA GAGAGAGAAG AAAGTGTGGG AGGAGCATTT GGTGGCCAAG 
GGAATAAAGA ACTCTGATGT GTCTCCGCCG TCAGGGCAGC AGGAAATGGG GGGTTCCCCA 
ACAAAGCAGC CAATGAGACC TGTTATTTCT GTGACTTCGG CTTTGAAGGA AGTGTGTATG 
GACCAAAGTC TAACTGATGC CCAAGAAACC TCAGAAGAAA TGCAGAAGAG TAACAGTGCT 
GTTTCAAGTA AACGAGAAAT ACTACGTAGA TTAAATCAAA ATCTTAAAGT TCAAGAAAAT 
GAAAAGGGAA GGCAGAGTCG TCGTGAAACG GGTACAGTAA CTGTTCATGA AGATGCCAAA 
GAGCTGGAAA AAGAAAAGTC AGTTGTATCG GATCGAAAAA AATGGGAGAC AGGAGGCCAG 
CTTGTGCTTC CTGTGGACGA ACTGGCCCTG GACGCTTCCT TCTCTGTGAC AGAAAAACAT 
ATAGTGGGAG AGGTTATTAA ATTAGATCCT AGTGGATCTC CAAGAAAAGT CTGGGGGAAA 
AGTCCTACAG ATTCTGTTCT GAGGATACTT GGAGAAGCTG AACTACAACT TCAGACAGAA 
CTACTGGAAA ACACATCTAT TAGAAATGAG ATTTCTCCTG AAAGCGAGAA TTTCAAATCC 
TTAATTACTG AAGAAGAAAA AGTAAAATGT ATTCCACTTG AAATGAACCC ACCAGCTACT 
CTTGATTCTT CTGTTGAGAC AAACAGTCCA AACATTAGTG AAGCATCTCC ACAGACAGCA 
TTGAAAGCAG AAGGAGATTT GGAGGAGCCT GATGACTTGG AGGCAGAAGT GCTGCAGGAG 
CCAAGTGGAG CAGACAGAGA TGGTGGTTTG CCCCAACCTG TTCTCGACAT GTGGGTCACT 
GAGAGAGAAG GAGCAGAGGA GGTTGAATTG GAAGATAGGA TCACCATTCA GCAAAATGAA 
CTTTCTGAAG ATAAAGCCCC GAGCAGTGTG GATCACCTTA GTGAGGTTCA TGTAGAACCT 
GGAGTGGATG ATTCTCCACC CTCTAAGCGC GATATAGATA AATCTATGCA ACCAGAACCA 
TTTTTCCATA AAGTGGTTTA TTCTGAGCAT CTGAAAGCAG TTTCTCAGGT TCAGTCAGTT 
CTCTGTTCAC CAGAAGAATC CTTTCCACTT CGATCTCACT CTGATTCACC ACCAAGGAAT 
AAAAAACAGA ATTCCTTGCT GATTGGACTT TCAACGGGTC TATTTGATGC AAACAACCCA 
AAGATGTTGC GGACATGTTC ACTTCCAGAC CTCTCCAAAC TGTTCAGAAC ACTGATGGAT 
GTCCCCACTG TGGGAGATGG ACGTCAGGAC AATCTTGAAA TGGAAGATGA GCATGTTAAG 
GAAGGGCCGT CGGACTCTGA GGACATTGTG TTTGAAGAGG CTGACACAGA CTTACAGGAG 
CTCCAAGCCT CCATGGAGCA GCTGCTCCAG GAGCAGCCAG GGGAGGACTA CAGCGAAGAG 
GAGGAGTCGG GCTTGAAGAC CAGTGATGAC GCCGAGCAGG CCGCCAACGG CACGGACGCG 
GGCGAGGAGG ACGACAACCC CAGCAGCGAG AGCGCCCTCA ATGAGGAGTG GCACTCGGAT 
AACAGTGATG GGGAGATAAC TAGTGAATGT GAATACGATA GTGTCTTTAA TCATTTAGAG 
GAACTGAGGC TTCACCTGGA ACAGGAAATA GGCTTCGAAA AGTTCTTTGA GGTTTATGAA 
AAAATAAAGG CTGTTCATGA AGATGAAGAT GAAAATATTG AGATTTGTTC AACAATAGTT 
CAGAATATTT TGGGAAATGA ACATCAGCAT CTTTATGCCA AGATTCTTCA TTTAGTCATG 
GCAGATGGAG CCTATCAAGA AGATAATGAT GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_015464416.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015319902.1 Bos taurus NIMA related kinase 1 (NEK1), transcript variant X4, mRNA.

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CloneID	OBa52912
Clone ID Related Accession (Same CDS sequence)	XM_015464416.1 , XM_010807678.1 , XM_010807678.2
Accession Version	XM_015464416.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3576bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	serine/threonine-protein kinase Nek1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645187.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAAT ACATTAGAGT ACAGAAGATT GGAGAAGGTT CATTCGGAAA AGCCATTCTT 
GTCAGATCTA CAGAAGATGG CAGACAATAT GTCATCAAAG AAATTAATAT CTCAAGGATG 
TCCAATAAAG AGAGGGAAGA ATCAAGGAGA GAAGTTGCAG TGCTAGCAAA CATGCGGCAT 
CCAAACATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATTGTAATG 
GATTACTGTG AAGGAGGGGA TCTGTTCAAA AGAATAAATG CTCAGAAGGG CGTTTTGTTT 
CAAGAGGACC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTAAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATAAAA TCACAGAACA TATTTTTAAC CAGAGATGGA 
ACAATACAAC TTGGAGATTT TGGAATCGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA TAAACCTTAT 
AATAATAAAA GTGATATTTG GGCTCTGGGG TGTGTCCTTT ATGAGATGTG CACACTTAAA 
CATGCATTTG AAGCTGGCAA TATGAAGAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCCGTGT CTTTGCATTA TTCTTACGAT CTCCGCAGTT TACTCTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC CCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCCAAGTTTG GAGCACAACC TCTACCAGCT AAAAGACCAG CTTCAGGACA AAGCTTGGCT 
TCTATTATGT CTGCTCAGAA AATTACAAAG CCTGCTGCTA AATATGGAAT ACCGTTAACA 
AATAAGAAAT ATATAGATAA AAAATTGCAT GAAAGGAAGC CACTCCAGAA ATATAAACAG 
GCCCATCAAA CCCCAGTGAA GAAAGTGAAT CCTGGAGAAG AAAGGAGGAA ACTGTTTGAG 
GAAGCACCAA GAAAAAGAAG ATTGGAATTG ACTGAAAAAG AAAAGAAGTC TAAGGATCAG 
GTTAGTCTGC TGAAGGCTGA GCAGGTGAAA AGACAGGAAA AGCAAAGGTT GGAGAGAATA 
AATAGGGCCA GGGAACAAGG CTGGAGAAAT GTGCTGAGTG CTGGTGGAAG CGGTGAAATG 
AAGGCTCCCT TGTTGGGCAG TGGAGGGGCC ATGGCTCCAT CATCTTTTTC CTCTCGAGGA 
CAGTATGAAC ATTATCACGC TATTTTTGAT CAAATGCAGC AACAAAGAGC AGAAGATAAT 
GAAGCTAAAT GGAAAGGAGA AATACATGGC CAGAGGCTTC CAGAAAGGCA GAAGGGGCAG 
CCAGCTATGG AAAGAGCAAA ACAAGTAGAA GAGTTTCTAC AGCGAAGACG GGAAGCAAAG 
CAGAACAAAG CTCGGGCCGA GGGGCATATG GGCATCCTGC ACAACCTGGC AGCTCTCTAT 
GGAGGCAGGC CCAGCTCTTC ACGAGGAGGG AAGCCGAGAA ACAAAGAGGA AGAGGTTTAT 
TTGGCAAGAC TAAGACAAAT AAGACTGCAG AATTTCAATG AACGCCAACA GATTAGAGCC 
AAGCTTCGTG GTGAAAAGAA GGAAACTGAC AGCTCTGAAG ATCAAGAAGG AAGTGAGGAG 
GCTGATGTGA GGCGTAAAAA AGTGGAAGCC CTGAAGGCCC AGGCAAGTGC CCGTGCAGCC 
GTCCTGAAGG AGCAGCTTGA GCGCAAGCGG AAGGAGGCAT ATGAGAGAGA GAAGAAAGTG 
TGGGAGGAGC ATTTGGTGGC CAAGGGAATA AAGAACTCTG ATGTGTCTCC GCCGTCAGGG 
CAGCAGGAAA TGGGGGGTTC CCCAACAAAG CAGCCAATGA GACCTGTTAT TTCTGTGACT 
TCGGCTTTGA AGGAAGTGTG TATGGACCAA AGTCTAACTG ATGCCCAAGA AACCTCAGAA 
GAAATGCAGA AGAGTAACAG TGCTGTTTCA AGTAAACGAG AAATACTACG TAGATTAAAT 
CAAAATCTTA AAGTTCAAGA AAATGAAAAG GGAAGGCAGA GTCGTCGTGA AACGGAACAT 
ATAGTGGGAG AGGTTATTAA ATTAGATCCT AGTGGATCTC CAAGAAAAGT CTGGGGGAAA 
AGTCCTACAG ATTCTGTTCT GAGGATACTT GGAGAAGCTG AACTACAACT TCAGACAGAA 
CTACTGGAAA ACACATCTAT TAGAAATGAG ATTTCTCCTG AAAGCGAGAA TTTCAAATCC 
TTAATTACTG AAGAAGAAAA AGTAAAATGT ATTCCACTTG AAATGAACCC ACCAGCTACT 
CTTGATTCTT CTGTTGAGAC AAACAGTCCA AACATTAGTG AAGCATCTCC ACAGACAGCA 
TTGAAAGCAG AAGGAGATTT GGAGGAGCCT GATGACTTGG AGGCAGAAGT GCTGCAGGAG 
CCAAGTGGAG CAGACAGAGA TGGTGGTTTG CCCCAACCTG TTCTCGACAT GTGGGTCACT 
GAGAGAGAAG GAGCAGAGGA GGTTGAATTG GAAGATAGGA TCACCATTCA GCAAAATGAA 
CTTTCTGAAG ATAAAGCCCC GAGCAGTGTG GATCACCTTA GTGAGGTTCA TGTAGAACCT 
GGAGTGGATG ATTCTCCACC CTCTAAGCGC GATATAGATA AATCTATGCA ACCAGAACCA 
TTTTTCCATA AAGTGGTTTA TTCTGAGCAT CTGAAAGCAG TTTCTCAGGT TCAGTCAGTT 
CTCTGTTCAC CAGAAGAATC CTTTCCACTT CGATCTCACT CTGATTCACC ACCAAGGAAT 
AAAAAACAGA ATTCCTTGCT GATTGGACTT TCAACGGGTC TATTTGATGC AAACAACCCA 
AAGATGTTGC GGACATGTTC ACTTCCAGAC CTCTCCAAAC TGTTCAGAAC ACTGATGGAT 
GTCCCCACTG TGGGAGATGG ACGTCAGGAC AATCTTGAAA TGGAAGATGA GCATGTTAAG 
GAAGGGCCGT CGGACTCTGA GGACATTGTG TTTGAAGAGG CTGACACAGA CTTACAGGAG 
CTCCAAGCCT CCATGGAGCA GCTGCTCCAG GAGCAGCCAG GGGAGGACTA CAGCGAAGAG 
GAGGAGTCGG GCTTGAAGAC CAGTGATGAC GCCGAGCAGG CCGCCAACGG CACGGACGCG 
GGCGAGGAGG ACGACAACCC CAGCAGCGAG AGCGCCCTCA ATGAGGAGTG GCACTCGGAT 
AACAGTGATG GGGAGATAAC TAGTGAATGT GAATACGATA GTGTCTTTAA TCATTTAGAG 
GAACTGAGGC TTCACCTGGA ACAGGAAATA GGCTTCGAAA AGTTCTTTGA GGTTTATGAA 
AAAATAAAGG CTGTTCATGA AGATGAAGAT GAAAATATTG AGATTTGTTC AACAATAGTT 
CAGAATATTT TGGGAAATGA ACATCAGCAT CTTTATGCCA AGATTCTTCA TTTAGTCATG 
GCAGATGGAG CCTATCAAGA AGATAATGAT GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015319902.1
CDS	770..4345
Translation	MEKYIRVQKIGEGSFGKAILVRSTEDGRQYVIKEINISRMSNKEREESRREVAVLANMRHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGVLFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTRDGTIQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIISGSFPPVSLHYSYDLRSLLSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTFSKFGAQPLPAKRPASGQSLASIMSAQKITKPAAKYGIPLTNKKYIDKKLHERKPLQKYKQAHQTPVKKVNPGEERRKLFEEAPRKRRLELTEKEKKSKDQVSLLKAEQVKRQEKQRLERINRAREQGWRNVLSAGGSGEMKAPLLGSGGAMAPSSFSSRGQYEHYHAIFDQMQQQRAEDNEAKWKGEIHGQRLPERQKGQPAMERAKQVEEFLQRRREAKQNKARAEGHMGILHNLAALYGGRPSSSRGGKPRNKEEEVYLARLRQIRLQNFNERQQIRAKLRGEKKETDSSEDQEGSEEADVRRKKVEALKAQASARAAVLKEQLERKRKEAYEREKKVWEEHLVAKGIKNSDVSPPSGQQEMGGSPTKQPMRPVISVTSALKEVCMDQSLTDAQETSEEMQKSNSAVSSKREILRRLNQNLKVQENEKGRQSRRETEHIVGEVIKLDPSGSPRKVWGKSPTDSVLRILGEAELQLQTELLENTSIRNEISPESENFKSLITEEEKVKCIPLEMNPPATLDSSVETNSPNISEASPQTALKAEGDLEEPDDLEAEVLQEPSGADRDGGLPQPVLDMWVTEREGAEEVELEDRITIQQNELSEDKAPSSVDHLSEVHVEPGVDDSPPSKRDIDKSMQPEPFFHKVVYSEHLKAVSQVQSVLCSPEESFPLRSHSDSPPRNKKQNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDGRQDNLEMEDEHVKEGPSDSEDIVFEEADTDLQELQASMEQLLQEQPGEDYSEEEESGLKTSDDAEQAANGTDAGEEDDNPSSESALNEEWHSDNSDGEITSECEYDSVFNHLEELRLHLEQEIGFEKFFEVYEKIKAVHEDEDENIEICSTIVQNILGNEHQHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_015464416.1
Organism	Bos taurus(cattle)
Definition	Bos taurus NIMA related kinase 1 (NEK1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015464416.1

ORF Insert Sequence:

ATGGAGAAAT ACATTAGAGT ACAGAAGATT GGAGAAGGTT CATTCGGAAA AGCCATTCTT 
GTCAGATCTA CAGAAGATGG CAGACAATAT GTCATCAAAG AAATTAATAT CTCAAGGATG 
TCCAATAAAG AGAGGGAAGA ATCAAGGAGA GAAGTTGCAG TGCTAGCAAA CATGCGGCAT 
CCAAACATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATTGTAATG 
GATTACTGTG AAGGAGGGGA TCTGTTCAAA AGAATAAATG CTCAGAAGGG CGTTTTGTTT 
CAAGAGGACC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTAAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATAAAA TCACAGAACA TATTTTTAAC CAGAGATGGA 
ACAATACAAC TTGGAGATTT TGGAATCGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA TAAACCTTAT 
AATAATAAAA GTGATATTTG GGCTCTGGGG TGTGTCCTTT ATGAGATGTG CACACTTAAA 
CATGCATTTG AAGCTGGCAA TATGAAGAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCCGTGT CTTTGCATTA TTCTTACGAT CTCCGCAGTT TACTCTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC CCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCCAAGTTTG GAGCACAACC TCTACCAGCT AAAAGACCAG CTTCAGGACA AAGCTTGGCT 
TCTATTATGT CTGCTCAGAA AATTACAAAG CCTGCTGCTA AATATGGAAT ACCGTTAACA 
AATAAGAAAT ATATAGATAA AAAATTGCAT GAAAGGAAGC CACTCCAGAA ATATAAACAG 
GCCCATCAAA CCCCAGTGAA GAAAGTGAAT CCTGGAGAAG AAAGGAGGAA ACTGTTTGAG 
GAAGCACCAA GAAAAAGAAG ATTGGAATTG ACTGAAAAAG AAAAGAAGTC TAAGGATCAG 
GTTAGTCTGC TGAAGGCTGA GCAGGTGAAA AGACAGGAAA AGCAAAGGTT GGAGAGAATA 
AATAGGGCCA GGGAACAAGG CTGGAGAAAT GTGCTGAGTG CTGGTGGAAG CGGTGAAATG 
AAGGCTCCCT TGTTGGGCAG TGGAGGGGCC ATGGCTCCAT CATCTTTTTC CTCTCGAGGA 
CAGTATGAAC ATTATCACGC TATTTTTGAT CAAATGCAGC AACAAAGAGC AGAAGATAAT 
GAAGCTAAAT GGAAAGGAGA AATACATGGC CAGAGGCTTC CAGAAAGGCA GAAGGGGCAG 
CCAGCTATGG AAAGAGCAAA ACAAGTAGAA GAGTTTCTAC AGCGAAGACG GGAAGCAAAG 
CAGAACAAAG CTCGGGCCGA GGGGCATATG GGCATCCTGC ACAACCTGGC AGCTCTCTAT 
GGAGGCAGGC CCAGCTCTTC ACGAGGAGGG AAGCCGAGAA ACAAAGAGGA AGAGGTTTAT 
TTGGCAAGAC TAAGACAAAT AAGACTGCAG AATTTCAATG AACGCCAACA GATTAGAGCC 
AAGCTTCGTG GTGAAAAGAA GGAAACTGAC AGCTCTGAAG ATCAAGAAGG AAGTGAGGAG 
GCTGATGTGA GGCGTAAAAA AGTGGAAGCC CTGAAGGCCC AGGCAAGTGC CCGTGCAGCC 
GTCCTGAAGG AGCAGCTTGA GCGCAAGCGG AAGGAGGCAT ATGAGAGAGA GAAGAAAGTG 
TGGGAGGAGC ATTTGGTGGC CAAGGGAATA AAGAACTCTG ATGTGTCTCC GCCGTCAGGG 
CAGCAGGAAA TGGGGGGTTC CCCAACAAAG CAGCCAATGA GACCTGTTAT TTCTGTGACT 
TCGGCTTTGA AGGAAGTGTG TATGGACCAA AGTCTAACTG ATGCCCAAGA AACCTCAGAA 
GAAATGCAGA AGAGTAACAG TGCTGTTTCA AGTAAACGAG AAATACTACG TAGATTAAAT 
CAAAATCTTA AAGTTCAAGA AAATGAAAAG GGAAGGCAGA GTCGTCGTGA AACGGAACAT 
ATAGTGGGAG AGGTTATTAA ATTAGATCCT AGTGGATCTC CAAGAAAAGT CTGGGGGAAA 
AGTCCTACAG ATTCTGTTCT GAGGATACTT GGAGAAGCTG AACTACAACT TCAGACAGAA 
CTACTGGAAA ACACATCTAT TAGAAATGAG ATTTCTCCTG AAAGCGAGAA TTTCAAATCC 
TTAATTACTG AAGAAGAAAA AGTAAAATGT ATTCCACTTG AAATGAACCC ACCAGCTACT 
CTTGATTCTT CTGTTGAGAC AAACAGTCCA AACATTAGTG AAGCATCTCC ACAGACAGCA 
TTGAAAGCAG AAGGAGATTT GGAGGAGCCT GATGACTTGG AGGCAGAAGT GCTGCAGGAG 
CCAAGTGGAG CAGACAGAGA TGGTGGTTTG CCCCAACCTG TTCTCGACAT GTGGGTCACT 
GAGAGAGAAG GAGCAGAGGA GGTTGAATTG GAAGATAGGA TCACCATTCA GCAAAATGAA 
CTTTCTGAAG ATAAAGCCCC GAGCAGTGTG GATCACCTTA GTGAGGTTCA TGTAGAACCT 
GGAGTGGATG ATTCTCCACC CTCTAAGCGC GATATAGATA AATCTATGCA ACCAGAACCA 
TTTTTCCATA AAGTGGTTTA TTCTGAGCAT CTGAAAGCAG TTTCTCAGGT TCAGTCAGTT 
CTCTGTTCAC CAGAAGAATC CTTTCCACTT CGATCTCACT CTGATTCACC ACCAAGGAAT 
AAAAAACAGA ATTCCTTGCT GATTGGACTT TCAACGGGTC TATTTGATGC AAACAACCCA 
AAGATGTTGC GGACATGTTC ACTTCCAGAC CTCTCCAAAC TGTTCAGAAC ACTGATGGAT 
GTCCCCACTG TGGGAGATGG ACGTCAGGAC AATCTTGAAA TGGAAGATGA GCATGTTAAG 
GAAGGGCCGT CGGACTCTGA GGACATTGTG TTTGAAGAGG CTGACACAGA CTTACAGGAG 
CTCCAAGCCT CCATGGAGCA GCTGCTCCAG GAGCAGCCAG GGGAGGACTA CAGCGAAGAG 
GAGGAGTCGG GCTTGAAGAC CAGTGATGAC GCCGAGCAGG CCGCCAACGG CACGGACGCG 
GGCGAGGAGG ACGACAACCC CAGCAGCGAG AGCGCCCTCA ATGAGGAGTG GCACTCGGAT 
AACAGTGATG GGGAGATAAC TAGTGAATGT GAATACGATA GTGTCTTTAA TCATTTAGAG 
GAACTGAGGC TTCACCTGGA ACAGGAAATA GGCTTCGAAA AGTTCTTTGA GGTTTATGAA 
AAAATAAAGG CTGTTCATGA AGATGAAGAT GAAAATATTG AGATTTGTTC AACAATAGTT 
CAGAATATTT TGGGAAATGA ACATCAGCAT CTTTATGCCA AGATTCTTCA TTTAGTCATG 
GCAGATGGAG CCTATCAAGA AGATAATGAT GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_010807678.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010805980.1 Bos taurus NIMA related kinase 1 (NEK1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa52912
Clone ID Related Accession (Same CDS sequence)	XM_015464416.1 , XM_010807678.1 , XM_010807678.2
Accession Version	XM_010807678.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3576bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	serine/threonine-protein kinase Nek1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104069.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAAT ACATTAGAGT ACAGAAGATT GGAGAAGGTT CATTCGGAAA AGCCATTCTT 
GTCAGATCTA CAGAAGATGG CAGACAATAT GTCATCAAAG AAATTAATAT CTCAAGGATG 
TCCAATAAAG AGAGGGAAGA ATCAAGGAGA GAAGTTGCAG TGCTAGCAAA CATGCGGCAT 
CCAAACATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATTGTAATG 
GATTACTGTG AAGGAGGGGA TCTGTTCAAA AGAATAAATG CTCAGAAGGG CGTTTTGTTT 
CAAGAGGACC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTAAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATAAAA TCACAGAACA TATTTTTAAC CAGAGATGGA 
ACAATACAAC TTGGAGATTT TGGAATCGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA TAAACCTTAT 
AATAATAAAA GTGATATTTG GGCTCTGGGG TGTGTCCTTT ATGAGATGTG CACACTTAAA 
CATGCATTTG AAGCTGGCAA TATGAAGAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCCGTGT CTTTGCATTA TTCTTACGAT CTCCGCAGTT TACTCTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC CCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCCAAGTTTG GAGCACAACC TCTACCAGCT AAAAGACCAG CTTCAGGACA AAGCTTGGCT 
TCTATTATGT CTGCTCAGAA AATTACAAAG CCTGCTGCTA AATATGGAAT ACCGTTAACA 
AATAAGAAAT ATATAGATAA AAAATTGCAT GAAAGGAAGC CACTCCAGAA ATATAAACAG 
GCCCATCAAA CCCCAGTGAA GAAAGTGAAT CCTGGAGAAG AAAGGAGGAA ACTGTTTGAG 
GAAGCACCAA GAAAAAGAAG ATTGGAATTG ACTGAAAAAG AAAAGAAGTC TAAGGATCAG 
GTTAGTCTGC TGAAGGCTGA GCAGGTGAAA AGACAGGAAA AGCAAAGGTT GGAGAGAATA 
AATAGGGCCA GGGAACAAGG CTGGAGAAAT GTGCTGAGTG CTGGTGGAAG CGGTGAAATG 
AAGGCTCCCT TGTTGGGCAG TGGAGGGGCC ATGGCTCCAT CATCTTTTTC CTCTCGAGGA 
CAGTATGAAC ATTATCACGC TATTTTTGAT CAAATGCAGC AACAAAGAGC AGAAGATAAT 
GAAGCTAAAT GGAAAGGAGA AATACATGGC CAGAGGCTTC CAGAAAGGCA GAAGGGGCAG 
CCAGCTATGG AAAGAGCAAA ACAAGTAGAA GAGTTTCTAC AGCGAAGACG GGAAGCAAAG 
CAGAACAAAG CTCGGGCCGA GGGGCATATG GGCATCCTGC ACAACCTGGC AGCTCTCTAT 
GGAGGCAGGC CCAGCTCTTC ACGAGGAGGG AAGCCGAGAA ACAAAGAGGA AGAGGTTTAT 
TTGGCAAGAC TAAGACAAAT AAGACTGCAG AATTTCAATG AACGCCAACA GATTAGAGCC 
AAGCTTCGTG GTGAAAAGAA GGAAACTGAC AGCTCTGAAG ATCAAGAAGG AAGTGAGGAG 
GCTGATGTGA GGCGTAAAAA AGTGGAAGCC CTGAAGGCCC AGGCAAGTGC CCGTGCAGCC 
GTCCTGAAGG AGCAGCTTGA GCGCAAGCGG AAGGAGGCAT ATGAGAGAGA GAAGAAAGTG 
TGGGAGGAGC ATTTGGTGGC CAAGGGAATA AAGAACTCTG ATGTGTCTCC GCCGTCAGGG 
CAGCAGGAAA TGGGGGGTTC CCCAACAAAG CAGCCAATGA GACCTGTTAT TTCTGTGACT 
TCGGCTTTGA AGGAAGTGTG TATGGACCAA AGTCTAACTG ATGCCCAAGA AACCTCAGAA 
GAAATGCAGA AGAGTAACAG TGCTGTTTCA AGTAAACGAG AAATACTACG TAGATTAAAT 
CAAAATCTTA AAGTTCAAGA AAATGAAAAG GGAAGGCAGA GTCGTCGTGA AACGGAACAT 
ATAGTGGGAG AGGTTATTAA ATTAGATCCT AGTGGATCTC CAAGAAAAGT CTGGGGGAAA 
AGTCCTACAG ATTCTGTTCT GAGGATACTT GGAGAAGCTG AACTACAACT TCAGACAGAA 
CTACTGGAAA ACACATCTAT TAGAAATGAG ATTTCTCCTG AAAGCGAGAA TTTCAAATCC 
TTAATTACTG AAGAAGAAAA AGTAAAATGT ATTCCACTTG AAATGAACCC ACCAGCTACT 
CTTGATTCTT CTGTTGAGAC AAACAGTCCA AACATTAGTG AAGCATCTCC ACAGACAGCA 
TTGAAAGCAG AAGGAGATTT GGAGGAGCCT GATGACTTGG AGGCAGAAGT GCTGCAGGAG 
CCAAGTGGAG CAGACAGAGA TGGTGGTTTG CCCCAACCTG TTCTCGACAT GTGGGTCACT 
GAGAGAGAAG GAGCAGAGGA GGTTGAATTG GAAGATAGGA TCACCATTCA GCAAAATGAA 
CTTTCTGAAG ATAAAGCCCC GAGCAGTGTG GATCACCTTA GTGAGGTTCA TGTAGAACCT 
GGAGTGGATG ATTCTCCACC CTCTAAGCGC GATATAGATA AATCTATGCA ACCAGAACCA 
TTTTTCCATA AAGTGGTTTA TTCTGAGCAT CTGAAAGCAG TTTCTCAGGT TCAGTCAGTT 
CTCTGTTCAC CAGAAGAATC CTTTCCACTT CGATCTCACT CTGATTCACC ACCAAGGAAT 
AAAAAACAGA ATTCCTTGCT GATTGGACTT TCAACGGGTC TATTTGATGC AAACAACCCA 
AAGATGTTGC GGACATGTTC ACTTCCAGAC CTCTCCAAAC TGTTCAGAAC ACTGATGGAT 
GTCCCCACTG TGGGAGATGG ACGTCAGGAC AATCTTGAAA TGGAAGATGA GCATGTTAAG 
GAAGGGCCGT CGGACTCTGA GGACATTGTG TTTGAAGAGG CTGACACAGA CTTACAGGAG 
CTCCAAGCCT CCATGGAGCA GCTGCTCCAG GAGCAGCCAG GGGAGGACTA CAGCGAAGAG 
GAGGAGTCGG GCTTGAAGAC CAGTGATGAC GCCGAGCAGG CCGCCAACGG CACGGACGCG 
GGCGAGGAGG ACGACAACCC CAGCAGCGAG AGCGCCCTCA ATGAGGAGTG GCACTCGGAT 
AACAGTGATG GGGAGATAAC TAGTGAATGT GAATACGATA GTGTCTTTAA TCATTTAGAG 
GAACTGAGGC TTCACCTGGA ACAGGAAATA GGCTTCGAAA AGTTCTTTGA GGTTTATGAA 
AAAATAAAGG CTGTTCATGA AGATGAAGAT GAAAATATTG AGATTTGTTC AACAATAGTT 
CAGAATATTT TGGGAAATGA ACATCAGCAT CTTTATGCCA AGATTCTTCA TTTAGTCATG 
GCAGATGGAG CCTATCAAGA AGATAATGAT GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010805980.1
CDS	770..4345
Translation	MEKYIRVQKIGEGSFGKAILVRSTEDGRQYVIKEINISRMSNKEREESRREVAVLANMRHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGVLFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTRDGTIQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIISGSFPPVSLHYSYDLRSLLSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTFSKFGAQPLPAKRPASGQSLASIMSAQKITKPAAKYGIPLTNKKYIDKKLHERKPLQKYKQAHQTPVKKVNPGEERRKLFEEAPRKRRLELTEKEKKSKDQVSLLKAEQVKRQEKQRLERINRAREQGWRNVLSAGGSGEMKAPLLGSGGAMAPSSFSSRGQYEHYHAIFDQMQQQRAEDNEAKWKGEIHGQRLPERQKGQPAMERAKQVEEFLQRRREAKQNKARAEGHMGILHNLAALYGGRPSSSRGGKPRNKEEEVYLARLRQIRLQNFNERQQIRAKLRGEKKETDSSEDQEGSEEADVRRKKVEALKAQASARAAVLKEQLERKRKEAYEREKKVWEEHLVAKGIKNSDVSPPSGQQEMGGSPTKQPMRPVISVTSALKEVCMDQSLTDAQETSEEMQKSNSAVSSKREILRRLNQNLKVQENEKGRQSRRETEHIVGEVIKLDPSGSPRKVWGKSPTDSVLRILGEAELQLQTELLENTSIRNEISPESENFKSLITEEEKVKCIPLEMNPPATLDSSVETNSPNISEASPQTALKAEGDLEEPDDLEAEVLQEPSGADRDGGLPQPVLDMWVTEREGAEEVELEDRITIQQNELSEDKAPSSVDHLSEVHVEPGVDDSPPSKRDIDKSMQPEPFFHKVVYSEHLKAVSQVQSVLCSPEESFPLRSHSDSPPRNKKQNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDGRQDNLEMEDEHVKEGPSDSEDIVFEEADTDLQELQASMEQLLQEQPGEDYSEEEESGLKTSDDAEQAANGTDAGEEDDNPSSESALNEEWHSDNSDGEITSECEYDSVFNHLEELRLHLEQEIGFEKFFEVYEKIKAVHEDEDENIEICSTIVQNILGNEHQHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_010807678.1
Organism	Bos taurus(cattle)
Definition	Bos taurus NIMA related kinase 1 (NEK1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010807678.1

ORF Insert Sequence:

ATGGAGAAAT ACATTAGAGT ACAGAAGATT GGAGAAGGTT CATTCGGAAA AGCCATTCTT 
GTCAGATCTA CAGAAGATGG CAGACAATAT GTCATCAAAG AAATTAATAT CTCAAGGATG 
TCCAATAAAG AGAGGGAAGA ATCAAGGAGA GAAGTTGCAG TGCTAGCAAA CATGCGGCAT 
CCAAACATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATTGTAATG 
GATTACTGTG AAGGAGGGGA TCTGTTCAAA AGAATAAATG CTCAGAAGGG CGTTTTGTTT 
CAAGAGGACC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTAAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATAAAA TCACAGAACA TATTTTTAAC CAGAGATGGA 
ACAATACAAC TTGGAGATTT TGGAATCGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA TAAACCTTAT 
AATAATAAAA GTGATATTTG GGCTCTGGGG TGTGTCCTTT ATGAGATGTG CACACTTAAA 
CATGCATTTG AAGCTGGCAA TATGAAGAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCCGTGT CTTTGCATTA TTCTTACGAT CTCCGCAGTT TACTCTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC CCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCCAAGTTTG GAGCACAACC TCTACCAGCT AAAAGACCAG CTTCAGGACA AAGCTTGGCT 
TCTATTATGT CTGCTCAGAA AATTACAAAG CCTGCTGCTA AATATGGAAT ACCGTTAACA 
AATAAGAAAT ATATAGATAA AAAATTGCAT GAAAGGAAGC CACTCCAGAA ATATAAACAG 
GCCCATCAAA CCCCAGTGAA GAAAGTGAAT CCTGGAGAAG AAAGGAGGAA ACTGTTTGAG 
GAAGCACCAA GAAAAAGAAG ATTGGAATTG ACTGAAAAAG AAAAGAAGTC TAAGGATCAG 
GTTAGTCTGC TGAAGGCTGA GCAGGTGAAA AGACAGGAAA AGCAAAGGTT GGAGAGAATA 
AATAGGGCCA GGGAACAAGG CTGGAGAAAT GTGCTGAGTG CTGGTGGAAG CGGTGAAATG 
AAGGCTCCCT TGTTGGGCAG TGGAGGGGCC ATGGCTCCAT CATCTTTTTC CTCTCGAGGA 
CAGTATGAAC ATTATCACGC TATTTTTGAT CAAATGCAGC AACAAAGAGC AGAAGATAAT 
GAAGCTAAAT GGAAAGGAGA AATACATGGC CAGAGGCTTC CAGAAAGGCA GAAGGGGCAG 
CCAGCTATGG AAAGAGCAAA ACAAGTAGAA GAGTTTCTAC AGCGAAGACG GGAAGCAAAG 
CAGAACAAAG CTCGGGCCGA GGGGCATATG GGCATCCTGC ACAACCTGGC AGCTCTCTAT 
GGAGGCAGGC CCAGCTCTTC ACGAGGAGGG AAGCCGAGAA ACAAAGAGGA AGAGGTTTAT 
TTGGCAAGAC TAAGACAAAT AAGACTGCAG AATTTCAATG AACGCCAACA GATTAGAGCC 
AAGCTTCGTG GTGAAAAGAA GGAAACTGAC AGCTCTGAAG ATCAAGAAGG AAGTGAGGAG 
GCTGATGTGA GGCGTAAAAA AGTGGAAGCC CTGAAGGCCC AGGCAAGTGC CCGTGCAGCC 
GTCCTGAAGG AGCAGCTTGA GCGCAAGCGG AAGGAGGCAT ATGAGAGAGA GAAGAAAGTG 
TGGGAGGAGC ATTTGGTGGC CAAGGGAATA AAGAACTCTG ATGTGTCTCC GCCGTCAGGG 
CAGCAGGAAA TGGGGGGTTC CCCAACAAAG CAGCCAATGA GACCTGTTAT TTCTGTGACT 
TCGGCTTTGA AGGAAGTGTG TATGGACCAA AGTCTAACTG ATGCCCAAGA AACCTCAGAA 
GAAATGCAGA AGAGTAACAG TGCTGTTTCA AGTAAACGAG AAATACTACG TAGATTAAAT 
CAAAATCTTA AAGTTCAAGA AAATGAAAAG GGAAGGCAGA GTCGTCGTGA AACGGAACAT 
ATAGTGGGAG AGGTTATTAA ATTAGATCCT AGTGGATCTC CAAGAAAAGT CTGGGGGAAA 
AGTCCTACAG ATTCTGTTCT GAGGATACTT GGAGAAGCTG AACTACAACT TCAGACAGAA 
CTACTGGAAA ACACATCTAT TAGAAATGAG ATTTCTCCTG AAAGCGAGAA TTTCAAATCC 
TTAATTACTG AAGAAGAAAA AGTAAAATGT ATTCCACTTG AAATGAACCC ACCAGCTACT 
CTTGATTCTT CTGTTGAGAC AAACAGTCCA AACATTAGTG AAGCATCTCC ACAGACAGCA 
TTGAAAGCAG AAGGAGATTT GGAGGAGCCT GATGACTTGG AGGCAGAAGT GCTGCAGGAG 
CCAAGTGGAG CAGACAGAGA TGGTGGTTTG CCCCAACCTG TTCTCGACAT GTGGGTCACT 
GAGAGAGAAG GAGCAGAGGA GGTTGAATTG GAAGATAGGA TCACCATTCA GCAAAATGAA 
CTTTCTGAAG ATAAAGCCCC GAGCAGTGTG GATCACCTTA GTGAGGTTCA TGTAGAACCT 
GGAGTGGATG ATTCTCCACC CTCTAAGCGC GATATAGATA AATCTATGCA ACCAGAACCA 
TTTTTCCATA AAGTGGTTTA TTCTGAGCAT CTGAAAGCAG TTTCTCAGGT TCAGTCAGTT 
CTCTGTTCAC CAGAAGAATC CTTTCCACTT CGATCTCACT CTGATTCACC ACCAAGGAAT 
AAAAAACAGA ATTCCTTGCT GATTGGACTT TCAACGGGTC TATTTGATGC AAACAACCCA 
AAGATGTTGC GGACATGTTC ACTTCCAGAC CTCTCCAAAC TGTTCAGAAC ACTGATGGAT 
GTCCCCACTG TGGGAGATGG ACGTCAGGAC AATCTTGAAA TGGAAGATGA GCATGTTAAG 
GAAGGGCCGT CGGACTCTGA GGACATTGTG TTTGAAGAGG CTGACACAGA CTTACAGGAG 
CTCCAAGCCT CCATGGAGCA GCTGCTCCAG GAGCAGCCAG GGGAGGACTA CAGCGAAGAG 
GAGGAGTCGG GCTTGAAGAC CAGTGATGAC GCCGAGCAGG CCGCCAACGG CACGGACGCG 
GGCGAGGAGG ACGACAACCC CAGCAGCGAG AGCGCCCTCA ATGAGGAGTG GCACTCGGAT 
AACAGTGATG GGGAGATAAC TAGTGAATGT GAATACGATA GTGTCTTTAA TCATTTAGAG 
GAACTGAGGC TTCACCTGGA ACAGGAAATA GGCTTCGAAA AGTTCTTTGA GGTTTATGAA 
AAAATAAAGG CTGTTCATGA AGATGAAGAT GAAAATATTG AGATTTGTTC AACAATAGTT 
CAGAATATTT TGGGAAATGA ACATCAGCAT CTTTATGCCA AGATTCTTCA TTTAGTCATG 
GCAGATGGAG CCTATCAAGA AGATAATGAT GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_010807678.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010805980.1 Bos taurus NIMA related kinase 1 (NEK1), transcript variant X5, mRNA.

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CloneID	OBa52912
Clone ID Related Accession (Same CDS sequence)	XM_015464416.1 , XM_010807678.1 , XM_010807678.2
Accession Version	XM_010807678.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3576bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	serine/threonine-protein kinase Nek1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010807678.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAAT ACATTAGAGT ACAGAAGATT GGAGAAGGTT CATTCGGAAA AGCCATTCTT 
GTCAGATCTA CAGAAGATGG CAGACAATAT GTCATCAAAG AAATTAATAT CTCAAGGATG 
TCCAATAAAG AGAGGGAAGA ATCAAGGAGA GAAGTTGCAG TGCTAGCAAA CATGCGGCAT 
CCAAACATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATTGTAATG 
GATTACTGTG AAGGAGGGGA TCTGTTCAAA AGAATAAATG CTCAGAAGGG CGTTTTGTTT 
CAAGAGGACC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTAAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATAAAA TCACAGAACA TATTTTTAAC CAGAGATGGA 
ACAATACAAC TTGGAGATTT TGGAATCGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA TAAACCTTAT 
AATAATAAAA GTGATATTTG GGCTCTGGGG TGTGTCCTTT ATGAGATGTG CACACTTAAA 
CATGCATTTG AAGCTGGCAA TATGAAGAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCCGTGT CTTTGCATTA TTCTTACGAT CTCCGCAGTT TACTCTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC CCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCCAAGTTTG GAGCACAACC TCTACCAGCT AAAAGACCAG CTTCAGGACA AAGCTTGGCT 
TCTATTATGT CTGCTCAGAA AATTACAAAG CCTGCTGCTA AATATGGAAT ACCGTTAACA 
AATAAGAAAT ATATAGATAA AAAATTGCAT GAAAGGAAGC CACTCCAGAA ATATAAACAG 
GCCCATCAAA CCCCAGTGAA GAAAGTGAAT CCTGGAGAAG AAAGGAGGAA ACTGTTTGAG 
GAAGCACCAA GAAAAAGAAG ATTGGAATTG ACTGAAAAAG AAAAGAAGTC TAAGGATCAG 
GTTAGTCTGC TGAAGGCTGA GCAGGTGAAA AGACAGGAAA AGCAAAGGTT GGAGAGAATA 
AATAGGGCCA GGGAACAAGG CTGGAGAAAT GTGCTGAGTG CTGGTGGAAG CGGTGAAATG 
AAGGCTCCCT TGTTGGGCAG TGGAGGGGCC ATGGCTCCAT CATCTTTTTC CTCTCGAGGA 
CAGTATGAAC ATTATCACGC TATTTTTGAT CAAATGCAGC AACAAAGAGC AGAAGATAAT 
GAAGCTAAAT GGAAAGGAGA AATACATGGC CAGAGGCTTC CAGAAAGGCA GAAGGGGCAG 
CCAGCTATGG AAAGAGCAAA ACAAGTAGAA GAGTTTCTAC AGCGAAGACG GGAAGCAAAG 
CAGAACAAAG CTCGGGCCGA GGGGCATATG GGCATCCTGC ACAACCTGGC AGCTCTCTAT 
GGAGGCAGGC CCAGCTCTTC ACGAGGAGGG AAGCCGAGAA ACAAAGAGGA AGAGGTTTAT 
TTGGCAAGAC TAAGACAAAT AAGACTGCAG AATTTCAATG AACGCCAACA GATTAGAGCC 
AAGCTTCGTG GTGAAAAGAA GGAAACTGAC AGCTCTGAAG ATCAAGAAGG AAGTGAGGAG 
GCTGATGTGA GGCGTAAAAA AGTGGAAGCC CTGAAGGCCC AGGCAAGTGC CCGTGCAGCC 
GTCCTGAAGG AGCAGCTTGA GCGCAAGCGG AAGGAGGCAT ATGAGAGAGA GAAGAAAGTG 
TGGGAGGAGC ATTTGGTGGC CAAGGGAATA AAGAACTCTG ATGTGTCTCC GCCGTCAGGG 
CAGCAGGAAA TGGGGGGTTC CCCAACAAAG CAGCCAATGA GACCTGTTAT TTCTGTGACT 
TCGGCTTTGA AGGAAGTGTG TATGGACCAA AGTCTAACTG ATGCCCAAGA AACCTCAGAA 
GAAATGCAGA AGAGTAACAG TGCTGTTTCA AGTAAACGAG AAATACTACG TAGATTAAAT 
CAAAATCTTA AAGTTCAAGA AAATGAAAAG GGAAGGCAGA GTCGTCGTGA AACGGAACAT 
ATAGTGGGAG AGGTTATTAA ATTAGATCCT AGTGGATCTC CAAGAAAAGT CTGGGGGAAA 
AGTCCTACAG ATTCTGTTCT GAGGATACTT GGAGAAGCTG AACTACAACT TCAGACAGAA 
CTACTGGAAA ACACATCTAT TAGAAATGAG ATTTCTCCTG AAAGCGAGAA TTTCAAATCC 
TTAATTACTG AAGAAGAAAA AGTAAAATGT ATTCCACTTG AAATGAACCC ACCAGCTACT 
CTTGATTCTT CTGTTGAGAC AAACAGTCCA AACATTAGTG AAGCATCTCC ACAGACAGCA 
TTGAAAGCAG AAGGAGATTT GGAGGAGCCT GATGACTTGG AGGCAGAAGT GCTGCAGGAG 
CCAAGTGGAG CAGACAGAGA TGGTGGTTTG CCCCAACCTG TTCTCGACAT GTGGGTCACT 
GAGAGAGAAG GAGCAGAGGA GGTTGAATTG GAAGATAGGA TCACCATTCA GCAAAATGAA 
CTTTCTGAAG ATAAAGCCCC GAGCAGTGTG GATCACCTTA GTGAGGTTCA TGTAGAACCT 
GGAGTGGATG ATTCTCCACC CTCTAAGCGC GATATAGATA AATCTATGCA ACCAGAACCA 
TTTTTCCATA AAGTGGTTTA TTCTGAGCAT CTGAAAGCAG TTTCTCAGGT TCAGTCAGTT 
CTCTGTTCAC CAGAAGAATC CTTTCCACTT CGATCTCACT CTGATTCACC ACCAAGGAAT 
AAAAAACAGA ATTCCTTGCT GATTGGACTT TCAACGGGTC TATTTGATGC AAACAACCCA 
AAGATGTTGC GGACATGTTC ACTTCCAGAC CTCTCCAAAC TGTTCAGAAC ACTGATGGAT 
GTCCCCACTG TGGGAGATGG ACGTCAGGAC AATCTTGAAA TGGAAGATGA GCATGTTAAG 
GAAGGGCCGT CGGACTCTGA GGACATTGTG TTTGAAGAGG CTGACACAGA CTTACAGGAG 
CTCCAAGCCT CCATGGAGCA GCTGCTCCAG GAGCAGCCAG GGGAGGACTA CAGCGAAGAG 
GAGGAGTCGG GCTTGAAGAC CAGTGATGAC GCCGAGCAGG CCGCCAACGG CACGGACGCG 
GGCGAGGAGG ACGACAACCC CAGCAGCGAG AGCGCCCTCA ATGAGGAGTG GCACTCGGAT 
AACAGTGATG GGGAGATAAC TAGTGAATGT GAATACGATA GTGTCTTTAA TCATTTAGAG 
GAACTGAGGC TTCACCTGGA ACAGGAAATA GGCTTCGAAA AGTTCTTTGA GGTTTATGAA 
AAAATAAAGG CTGTTCATGA AGATGAAGAT GAAAATATTG AGATTTGTTC AACAATAGTT 
CAGAATATTT TGGGAAATGA ACATCAGCAT CTTTATGCCA AGATTCTTCA TTTAGTCATG 
GCAGATGGAG CCTATCAAGA AGATAATGAT GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010805980.1
CDS	1123..4698
Translation	MEKYIRVQKIGEGSFGKAILVRSTEDGRQYVIKEINISRMSNKEREESRREVAVLANMRHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGVLFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTRDGTIQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIISGSFPPVSLHYSYDLRSLLSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTFSKFGAQPLPAKRPASGQSLASIMSAQKITKPAAKYGIPLTNKKYIDKKLHERKPLQKYKQAHQTPVKKVNPGEERRKLFEEAPRKRRLELTEKEKKSKDQVSLLKAEQVKRQEKQRLERINRAREQGWRNVLSAGGSGEMKAPLLGSGGAMAPSSFSSRGQYEHYHAIFDQMQQQRAEDNEAKWKGEIHGQRLPERQKGQPAMERAKQVEEFLQRRREAKQNKARAEGHMGILHNLAALYGGRPSSSRGGKPRNKEEEVYLARLRQIRLQNFNERQQIRAKLRGEKKETDSSEDQEGSEEADVRRKKVEALKAQASARAAVLKEQLERKRKEAYEREKKVWEEHLVAKGIKNSDVSPPSGQQEMGGSPTKQPMRPVISVTSALKEVCMDQSLTDAQETSEEMQKSNSAVSSKREILRRLNQNLKVQENEKGRQSRRETEHIVGEVIKLDPSGSPRKVWGKSPTDSVLRILGEAELQLQTELLENTSIRNEISPESENFKSLITEEEKVKCIPLEMNPPATLDSSVETNSPNISEASPQTALKAEGDLEEPDDLEAEVLQEPSGADRDGGLPQPVLDMWVTEREGAEEVELEDRITIQQNELSEDKAPSSVDHLSEVHVEPGVDDSPPSKRDIDKSMQPEPFFHKVVYSEHLKAVSQVQSVLCSPEESFPLRSHSDSPPRNKKQNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDGRQDNLEMEDEHVKEGPSDSEDIVFEEADTDLQELQASMEQLLQEQPGEDYSEEEESGLKTSDDAEQAANGTDAGEEDDNPSSESALNEEWHSDNSDGEITSECEYDSVFNHLEELRLHLEQEIGFEKFFEVYEKIKAVHEDEDENIEICSTIVQNILGNEHQHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_010807678.2
Organism	Bos taurus(cattle)
Definition	Bos taurus NIMA related kinase 1 (NEK1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010807678.2

ORF Insert Sequence:

ATGGAGAAAT ACATTAGAGT ACAGAAGATT GGAGAAGGTT CATTCGGAAA AGCCATTCTT 
GTCAGATCTA CAGAAGATGG CAGACAATAT GTCATCAAAG AAATTAATAT CTCAAGGATG 
TCCAATAAAG AGAGGGAAGA ATCAAGGAGA GAAGTTGCAG TGCTAGCAAA CATGCGGCAT 
CCAAACATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATTGTAATG 
GATTACTGTG AAGGAGGGGA TCTGTTCAAA AGAATAAATG CTCAGAAGGG CGTTTTGTTT 
CAAGAGGACC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTAAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATAAAA TCACAGAACA TATTTTTAAC CAGAGATGGA 
ACAATACAAC TTGGAGATTT TGGAATCGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA TAAACCTTAT 
AATAATAAAA GTGATATTTG GGCTCTGGGG TGTGTCCTTT ATGAGATGTG CACACTTAAA 
CATGCATTTG AAGCTGGCAA TATGAAGAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCCGTGT CTTTGCATTA TTCTTACGAT CTCCGCAGTT TACTCTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC CCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCCAAGTTTG GAGCACAACC TCTACCAGCT AAAAGACCAG CTTCAGGACA AAGCTTGGCT 
TCTATTATGT CTGCTCAGAA AATTACAAAG CCTGCTGCTA AATATGGAAT ACCGTTAACA 
AATAAGAAAT ATATAGATAA AAAATTGCAT GAAAGGAAGC CACTCCAGAA ATATAAACAG 
GCCCATCAAA CCCCAGTGAA GAAAGTGAAT CCTGGAGAAG AAAGGAGGAA ACTGTTTGAG 
GAAGCACCAA GAAAAAGAAG ATTGGAATTG ACTGAAAAAG AAAAGAAGTC TAAGGATCAG 
GTTAGTCTGC TGAAGGCTGA GCAGGTGAAA AGACAGGAAA AGCAAAGGTT GGAGAGAATA 
AATAGGGCCA GGGAACAAGG CTGGAGAAAT GTGCTGAGTG CTGGTGGAAG CGGTGAAATG 
AAGGCTCCCT TGTTGGGCAG TGGAGGGGCC ATGGCTCCAT CATCTTTTTC CTCTCGAGGA 
CAGTATGAAC ATTATCACGC TATTTTTGAT CAAATGCAGC AACAAAGAGC AGAAGATAAT 
GAAGCTAAAT GGAAAGGAGA AATACATGGC CAGAGGCTTC CAGAAAGGCA GAAGGGGCAG 
CCAGCTATGG AAAGAGCAAA ACAAGTAGAA GAGTTTCTAC AGCGAAGACG GGAAGCAAAG 
CAGAACAAAG CTCGGGCCGA GGGGCATATG GGCATCCTGC ACAACCTGGC AGCTCTCTAT 
GGAGGCAGGC CCAGCTCTTC ACGAGGAGGG AAGCCGAGAA ACAAAGAGGA AGAGGTTTAT 
TTGGCAAGAC TAAGACAAAT AAGACTGCAG AATTTCAATG AACGCCAACA GATTAGAGCC 
AAGCTTCGTG GTGAAAAGAA GGAAACTGAC AGCTCTGAAG ATCAAGAAGG AAGTGAGGAG 
GCTGATGTGA GGCGTAAAAA AGTGGAAGCC CTGAAGGCCC AGGCAAGTGC CCGTGCAGCC 
GTCCTGAAGG AGCAGCTTGA GCGCAAGCGG AAGGAGGCAT ATGAGAGAGA GAAGAAAGTG 
TGGGAGGAGC ATTTGGTGGC CAAGGGAATA AAGAACTCTG ATGTGTCTCC GCCGTCAGGG 
CAGCAGGAAA TGGGGGGTTC CCCAACAAAG CAGCCAATGA GACCTGTTAT TTCTGTGACT 
TCGGCTTTGA AGGAAGTGTG TATGGACCAA AGTCTAACTG ATGCCCAAGA AACCTCAGAA 
GAAATGCAGA AGAGTAACAG TGCTGTTTCA AGTAAACGAG AAATACTACG TAGATTAAAT 
CAAAATCTTA AAGTTCAAGA AAATGAAAAG GGAAGGCAGA GTCGTCGTGA AACGGAACAT 
ATAGTGGGAG AGGTTATTAA ATTAGATCCT AGTGGATCTC CAAGAAAAGT CTGGGGGAAA 
AGTCCTACAG ATTCTGTTCT GAGGATACTT GGAGAAGCTG AACTACAACT TCAGACAGAA 
CTACTGGAAA ACACATCTAT TAGAAATGAG ATTTCTCCTG AAAGCGAGAA TTTCAAATCC 
TTAATTACTG AAGAAGAAAA AGTAAAATGT ATTCCACTTG AAATGAACCC ACCAGCTACT 
CTTGATTCTT CTGTTGAGAC AAACAGTCCA AACATTAGTG AAGCATCTCC ACAGACAGCA 
TTGAAAGCAG AAGGAGATTT GGAGGAGCCT GATGACTTGG AGGCAGAAGT GCTGCAGGAG 
CCAAGTGGAG CAGACAGAGA TGGTGGTTTG CCCCAACCTG TTCTCGACAT GTGGGTCACT 
GAGAGAGAAG GAGCAGAGGA GGTTGAATTG GAAGATAGGA TCACCATTCA GCAAAATGAA 
CTTTCTGAAG ATAAAGCCCC GAGCAGTGTG GATCACCTTA GTGAGGTTCA TGTAGAACCT 
GGAGTGGATG ATTCTCCACC CTCTAAGCGC GATATAGATA AATCTATGCA ACCAGAACCA 
TTTTTCCATA AAGTGGTTTA TTCTGAGCAT CTGAAAGCAG TTTCTCAGGT TCAGTCAGTT 
CTCTGTTCAC CAGAAGAATC CTTTCCACTT CGATCTCACT CTGATTCACC ACCAAGGAAT 
AAAAAACAGA ATTCCTTGCT GATTGGACTT TCAACGGGTC TATTTGATGC AAACAACCCA 
AAGATGTTGC GGACATGTTC ACTTCCAGAC CTCTCCAAAC TGTTCAGAAC ACTGATGGAT 
GTCCCCACTG TGGGAGATGG ACGTCAGGAC AATCTTGAAA TGGAAGATGA GCATGTTAAG 
GAAGGGCCGT CGGACTCTGA GGACATTGTG TTTGAAGAGG CTGACACAGA CTTACAGGAG 
CTCCAAGCCT CCATGGAGCA GCTGCTCCAG GAGCAGCCAG GGGAGGACTA CAGCGAAGAG 
GAGGAGTCGG GCTTGAAGAC CAGTGATGAC GCCGAGCAGG CCGCCAACGG CACGGACGCG 
GGCGAGGAGG ACGACAACCC CAGCAGCGAG AGCGCCCTCA ATGAGGAGTG GCACTCGGAT 
AACAGTGATG GGGAGATAAC TAGTGAATGT GAATACGATA GTGTCTTTAA TCATTTAGAG 
GAACTGAGGC TTCACCTGGA ACAGGAAATA GGCTTCGAAA AGTTCTTTGA GGTTTATGAA 
AAAATAAAGG CTGTTCATGA AGATGAAGAT GAAAATATTG AGATTTGTTC AACAATAGTT 
CAGAATATTT TGGGAAATGA ACATCAGCAT CTTTATGCCA AGATTCTTCA TTTAGTCATG 
GCAGATGGAG CCTATCAAGA AGATAATGAT GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	NEK1
Entrez Gene ID	534671
Full Name	NIMA related kinase 1
General protein information	Preferred Names NIMA related kinase 1 Names serine/threonine-protein kinase Nek1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Bos taurus (cattle)	NEK1	XP_003586402.2
Rattus norvegicus (Norway rat)	Nek1	NP_001099552.2
Gallus gallus (chicken)	NEK1	XP_420401.3
Homo sapiens (human)	NEK1	NP_001186326.1
	NEK1	XP_001083275.1
Canis lupus familiaris (dog)	NEK1	XP_543184.2
Mus musculus (house mouse)	Nek1	NP_780298.2
Pan troglodytes (chimpanzee)	NEK1	XP_526727.2
Danio rerio (zebrafish)	nek1	XP_005160601.1
Xenopus tropicalis (tropical clawed frog)	nek1	NP_001090680.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

NEK1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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