Nek1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nek1
Entrez Gene ID	290705
Full Name	NIMA-related kinase 1
General protein information	Preferred Names NIMA-related kinase 1 Names serine/threonine-protein kinase Nek1 NIMA (never in mitosis gene a)-related expressed kinase 1 NIMA (never in mitosis gene a)-related kinase 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 16 Map Location: 16p12

mRNA and Protein(s)

mRNA	Protein	Name
NM_001106082.2	NP_001099552.2	serine/threonine-protein kinase Nek1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	NEK1	XP_003586402.2
Rattus norvegicus (Norway rat)	Nek1	NP_001099552.2
Gallus gallus (chicken)	NEK1	XP_420401.3
Homo sapiens (human)	NEK1	NP_001186326.1
	NEK1	XP_001083275.1
Canis lupus familiaris (dog)	NEK1	XP_543184.2
Mus musculus (house mouse)	Nek1	NP_780298.2
Pan troglodytes (chimpanzee)	NEK1	XP_526727.2
Danio rerio (zebrafish)	nek1	XP_005160601.1
Xenopus tropicalis (tropical clawed frog)	nek1	NP_001090680.1

Gene Ontology
Bibliography

Related articles in PubMed

Analysis of a cAMP regulated coactivator family reveals an alternative phosphorylation motif for AMPK family members.
Sonntag T, Moresco JJ, Vaughan JM, Matsumura S, Yates JR 3rd, Montminy M
PloS one12(2)e0173013(2017)

NEK1 and DYNC2H1 are both involved in short rib polydactyly Majewski type but not in Beemer Langer cases.
El Hokayem J, Huber C, Couv? A, Aziza J, Baujat G, Bouvier R, Cavalcanti DP, Collins FA, Cordier MP, Delezoide AL, Gonzales M, Johnson D, Le Merrer M, Levy-Mozziconacci A, Loget P, Martin-Coignard D, Martinovic J, Mortier GR, Perez MJ, Roume J, Scarano G, Munnich A, Cormier-Daire V
Journal of medical genetics49(4)227-33(2012 Apr)

Novel asymmetrically localizing components of human centrosomes identified by complementary proteomics methods.
Jakobsen L, Vanselow K, Skogs M, Toyoda Y, Lundberg E, Poser I, Falkenby LG, Bennetzen M, Westendorf J, Nigg EA, Uhlen M, Hyman AA, Andersen JS
The EMBO journal30(8)1520-35(2011 Apr)

NEK1 mutations cause short-rib polydactyly syndrome type majewski.
Thiel C, Kessler K, Giessl A, Dimmler A, Shalev SA, von der Haar S, Zenker M, Zahnleiter D, St?ss H, Beinder E, Abou Jamra R, Ekici AB, Schr?der-Kress N, Aigner T, Kirchner T, Reis A, Brandst?tter JH, Rauch A
American journal of human genetics88(1)106-14(2011 Jan)

The NIMA-related kinase NEK1 cycles through the nucleus.
Hilton LK, White MC, Quarmby LM
Biochemical and biophysical research communications389(1)52-6(2009 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Nek1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nek1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa30205	NM_001106082.2 Latest version!	Rattus norvegicus NIMA-related kinase 1 (Nek1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$384.30 $549.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nek1 ( NM_001106082.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001099552.2 Rattus norvegicus NIMA-related kinase 1 (Nek1), mRNA.

Price & Availability↑

CloneID	ORa30205
Clone ID Related Accession (Same CDS sequence)	NM_001106082.2
Accession Version	NM_001106082.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3603bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-28
Organism	Rattus norvegicus(Norway rat)
Product	serine/threonine-protein kinase Nek1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAHX01087277.1 and AAHX01087276.1. On Oct 27, 2010 this sequence version replaced NM_001106082.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCTGTTCTT 
GTTAAATCTA CAGAGGATGG CAGACATTAT GTCATCAAGG AAATTAACAT CTCAAGAATG 
TCCGATAAAG AAAGAGAAGA ATCAAGGAGA GAGGTCGCAG TGTTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAATACAA AGAATCATTC GAAGAAAATG GCTCTCTCTA CATAGTAATG 
GATTACTGTG AAGGAGGTGA TCTGTTTAAA CGAATAAATG CTCAGAAAGG CACTTTGTTT 
CAAGAAGATC AGATTTTGGA CTGGTTTGTT CAGATATGTT TGGCTTTGAA GCATGTACAC 
GATAGAAAAA TTCTTCACCG AGACATAAAG TCACAGAACA TATTTCTAAC CAAAGATGGG 
ACAGTGCAGC TTGGAGATTT TGGAATTGCT CGAGTTCTCA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACTCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAGCCTTAC 
AACAATAAAA GTGACATTTG GGCTTTGGGC TGTGTCCTTT ATGAGTTGTG TACACTTAAA 
CATGCATTTG AAGCTGGAAA CATGAAAAAC CTGGTACTGA AGATAATATC TGGATCCTTT 
CCTCCAGTGT CTCTACATTA TTCCTATGAT CTCCGCAGCT TGCTCTCTCA GCTGTTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGCTT TATAGCTAAA 
CGAATCGAAA AGTTTCTCTC ACCTCAGCTT ATTGCAGAAG AATTTTGTCT GAAAACAGTT 
TCAAAGTTTG GACCACAGCC TATCCCAGGT AAAAGACCAG CATCAGGACA AGGTGTCAGT 
TCTTTTGTCC CTGCTCAGAA AATCACAAAG CCTTCTGCTA AATATGGAGT GCCTATAACA 
TATAAGAAGT ACGGAGATAA AAAGTTACTT GAAAAAAAAC CACCCCCAAA GCATAAGCAG 
GCCCATCAGA TTCCAGTGAA GAAAATGAAT TCTGGAGAAG AAAGGAAGAA AATGTCTGAG 
GAAGCAGCAA AAAAACGAAG GTTGGAATTT ATTGAGAAAG AAAAGAAGCA AAAGGATCAG 
ATCAGGTTCC TGAAGGCTGA GCAGCTGAAG AGGCAAGAGA AGCAGCGGTT GGAGAGGATA 
AATAGGGCCA GGGAACAAGG ATGGAGGAAT GTTTTAAGGG CTGGTGGAAG CGGTGAAGTA 
AAGGCTTCCT TTTTTGGCAG TGGAGGAGCT GTGTCTCCAT CACCTTGTTC TCCTCGGGGC 
CAGTATGAAC ATTACCATGC CATCTTTGAC CAAATGCAGC AGCTAAGAGC AGAAGATAAT 
GAAGCGAGAT GGAAAGGGAG AATATATGGT CGATGGCTCC CTGAAAGGCA AAAAGGACAC 
CTAGGTGTAG AGAGAGCCAA ACAAGTGGAA GAATTCCTAC AGCGTAAACA AGAAGCTATG 
CAGAATAAAG CCCGAGCAGA AGGACATGTG GTTTATCTGG CAAGACTGAG GCAAATAAGA 
CTACAAAATT TTAATGAGCG CCAACAGATT AAAGCCAAAC TTCGTGGTGA GAATAAAGAA 
GCTGGTGGTA CCAAAGGACA AGACGCAACT GAAGAGGCTG ACATGAGGCT CAAAAAGATG 
GAGTCACTTA AGGCCCAAGC AAATGCACGT GCTGCTGTAC TAAAAGAACA GCTAGAGCGA 
AAAAGAAAGG AAGCTTATGA AAGAGAAAAG AAAGTGTGGG AAGAACATTT GGTGGTGAGG 
GTAAAAAGCT CAGATGTCCC TCCATCTATG GAGCATCATG AAGCAGGTGG TTCTCCATCA 
CAGCAGCAGA TGAAACCTGT TATTTCTGTG ACTTCAGCTT TGAAAGAAGT GGGCCTGGAT 
GGAAGTTTAA CTGAGTCCCA AGAAACTTCA GAAGAAATGG AAAAGAGTAA CAATGCTATT 
TCAAGTAAGC GAGAAATACT GCGTAGGCTG AATGAAAATC TTAAAGCTCA AGAGGATGAA 
AAGGAAAAGC ATCATCACGC TGATTCTTGT GAGACCGTTG GTCACAGAGA TGAGAGAGAG 
TATGAGAAGG AAAATGCCAT TTCCTCTGAT CGCAAGAAAT GGGAGATGGG AGGTCAGCTT 
GTGATTCCTC TGGATGAAGT GACACTGGAT GCATCCTTCT CTGCTTCTGA AAAACATACT 
GTGGGAGAGG TTATTAAGTT AGATTCTAGT GGCTCTCCAA GAAAAGTCTG GGGGAAAAGC 
CCTACAGATT CTGTGCTGAA GATACTTGGA GAAGCTGAAT TACAGCTACA GACAGAACTA 
CTAGAAAACA CGCCTTTTAA AAGTGAGGTT TGTGTTGAAG AGGAGAACTA CAAACCCCTA 
CTCACTGGAG AAGAGAATCT GCAGTGTATT TCAAAAGAAG TAAACCCATC AGCCACTGTT 
GATACTGAAA CAAACAGTCC AAAGTTTGCT AAGGTGTCTC CAGCAATGTC CGAAGGAAAT 
GTGGAAGAAC CTGATGATTT GGAAACAGAA GTTCTACAAG AGCCAAGTGC CATACCCTCA 
GATGGGAGTT TGCCGCCTGT TGTTAATGAG GTGTGGACCA GAGCTAAGGC AACTGGGTTG 
GAGGATAGCA TTGCTGTGCA GCAGAATGAC GTTTGTGAAG AGCGAATTCC AGGGACTGTG 
GACCAGTCCT GTAAGGATCA TATAGAACCT GCAGGAAACG ATTCTCCGCA GTCTGGCTGT 
GATGAAGAGA AGTCAGTACA GCCAGAATTG TTTTTCCAGA AAGTGGTTCA CTCCAAGGAC 
TTGAACTTAG TTCAGTCAGC TCATCGTTCC CCAGAAGAAG CAATTCCAAT TCGATCTCAC 
TCTGATTCTC CACCAAAAGC TAAAAGCAAG AATTCCCTAC TGATTGGACT TTCCACTGGT 
CTGTTTGATG CAAACAACCC AAAGATGCTG AGGACCTGTT CACTTCCAGA CCTTTCCAAG 
CTCTTCAGAA CCCTAATGGA TGTTCCCACT GTGGGAGACG TTCATCAAGA CAGTCTTGAA 
ATAGAGGAGC TTGAGGATGA ACATATTAAA GAAGGGCCCT CGGATTGCGA GGACATTGTA 
TTTGAAGAAA CTGACACAGA TTTACAGGAG CTTCAGGCCT CAATGGAGCA GCTGCTTAGG 
GAGCAACCCG GTGACGAATA CAGTGAGGAG GAAGAGTCTG TCTTAAAAAG CAGCAATGTG 
GAGCGGGCAG CAAGAGGGAC AGATGTCCCA GATGAGGAGG ACAACCCCAG CAGTGAAAGC 
GCCCTAAACG AGGAATGGCA CTCAGATAAC AGTGATGCTG AGACTACTAG TGAGTGTGAA 
TATGACAGTG TCTTTAACCA TTTAGAGGAA CTAAGACTTC ACTTGGAACA AGAAATGGGC 
TTTGAAAAGT TCTTTGAGGT CTATGAGAAA GTAAAGGCTA TTCATGAGGA TGAAGATGAA 
AATATTGAAA TTTCTTCAAC CATAGTCGAG AACATCTTGG GCACTGAGCA TCAGCACCTT 
TATGCCAAGA TTCTGCATCT AGTCATGGCA GACGGAGCCT ATCAGGAAGA TAATGATGAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001099552.2
CDS	110..3712
Translation	MEKYVRLQKIGEGSFGKAVLVKSTEDGRHYVIKEINISRMSDKEREESRREVAVLANMKHPNIVQYKESFEENGSLYIVMDYCEGGDLFKRINAQKGTLFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYELCTLKHAFEAGNMKNLVLKIISGSFPPVSLHYSYDLRSLLSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTVSKFGPQPIPGKRPASGQGVSSFVPAQKITKPSAKYGVPITYKKYGDKKLLEKKPPPKHKQAHQIPVKKMNSGEERKKMSEEAAKKRRLEFIEKEKKQKDQIRFLKAEQLKRQEKQRLERINRAREQGWRNVLRAGGSGEVKASFFGSGGAVSPSPCSPRGQYEHYHAIFDQMQQLRAEDNEARWKGRIYGRWLPERQKGHLGVERAKQVEEFLQRKQEAMQNKARAEGHVVYLARLRQIRLQNFNERQQIKAKLRGENKEAGGTKGQDATEEADMRLKKMESLKAQANARAAVLKEQLERKRKEAYEREKKVWEEHLVVRVKSSDVPPSMEHHEAGGSPSQQQMKPVISVTSALKEVGLDGSLTESQETSEEMEKSNNAISSKREILRRLNENLKAQEDEKEKHHHADSCETVGHRDEREYEKENAISSDRKKWEMGGQLVIPLDEVTLDASFSASEKHTVGEVIKLDSSGSPRKVWGKSPTDSVLKILGEAELQLQTELLENTPFKSEVCVEEENYKPLLTGEENLQCISKEVNPSATVDTETNSPKFAKVSPAMSEGNVEEPDDLETEVLQEPSAIPSDGSLPPVVNEVWTRAKATGLEDSIAVQQNDVCEERIPGTVDQSCKDHIEPAGNDSPQSGCDEEKSVQPELFFQKVVHSKDLNLVQSAHRSPEEAIPIRSHSDSPPKAKSKNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDVHQDSLEIEELEDEHIKEGPSDCEDIVFEETDTDLQELQASMEQLLREQPGDEYSEEEESVLKSSNVERAARGTDVPDEEDNPSSESALNEEWHSDNSDAETTSECEYDSVFNHLEELRLHLEQEMGFEKFFEVYEKVKAIHEDEDENIEISSTIVENILGTEHQHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	NM_001106082.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus NIMA-related kinase 1 (Nek1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001106082.2

ORF Insert Sequence:

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCTGTTCTT 
GTTAAATCTA CAGAGGATGG CAGACATTAT GTCATCAAGG AAATTAACAT CTCAAGAATG 
TCCGATAAAG AAAGAGAAGA ATCAAGGAGA GAGGTCGCAG TGTTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAATACAA AGAATCATTC GAAGAAAATG GCTCTCTCTA CATAGTAATG 
GATTACTGTG AAGGAGGTGA TCTGTTTAAA CGAATAAATG CTCAGAAAGG CACTTTGTTT 
CAAGAAGATC AGATTTTGGA CTGGTTTGTT CAGATATGTT TGGCTTTGAA GCATGTACAC 
GATAGAAAAA TTCTTCACCG AGACATAAAG TCACAGAACA TATTTCTAAC CAAAGATGGG 
ACAGTGCAGC TTGGAGATTT TGGAATTGCT CGAGTTCTCA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACTCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAGCCTTAC 
AACAATAAAA GTGACATTTG GGCTTTGGGC TGTGTCCTTT ATGAGTTGTG TACACTTAAA 
CATGCATTTG AAGCTGGAAA CATGAAAAAC CTGGTACTGA AGATAATATC TGGATCCTTT 
CCTCCAGTGT CTCTACATTA TTCCTATGAT CTCCGCAGCT TGCTCTCTCA GCTGTTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGCTT TATAGCTAAA 
CGAATCGAAA AGTTTCTCTC ACCTCAGCTT ATTGCAGAAG AATTTTGTCT GAAAACAGTT 
TCAAAGTTTG GACCACAGCC TATCCCAGGT AAAAGACCAG CATCAGGACA AGGTGTCAGT 
TCTTTTGTCC CTGCTCAGAA AATCACAAAG CCTTCTGCTA AATATGGAGT GCCTATAACA 
TATAAGAAGT ACGGAGATAA AAAGTTACTT GAAAAAAAAC CACCCCCAAA GCATAAGCAG 
GCCCATCAGA TTCCAGTGAA GAAAATGAAT TCTGGAGAAG AAAGGAAGAA AATGTCTGAG 
GAAGCAGCAA AAAAACGAAG GTTGGAATTT ATTGAGAAAG AAAAGAAGCA AAAGGATCAG 
ATCAGGTTCC TGAAGGCTGA GCAGCTGAAG AGGCAAGAGA AGCAGCGGTT GGAGAGGATA 
AATAGGGCCA GGGAACAAGG ATGGAGGAAT GTTTTAAGGG CTGGTGGAAG CGGTGAAGTA 
AAGGCTTCCT TTTTTGGCAG TGGAGGAGCT GTGTCTCCAT CACCTTGTTC TCCTCGGGGC 
CAGTATGAAC ATTACCATGC CATCTTTGAC CAAATGCAGC AGCTAAGAGC AGAAGATAAT 
GAAGCGAGAT GGAAAGGGAG AATATATGGT CGATGGCTCC CTGAAAGGCA AAAAGGACAC 
CTAGGTGTAG AGAGAGCCAA ACAAGTGGAA GAATTCCTAC AGCGTAAACA AGAAGCTATG 
CAGAATAAAG CCCGAGCAGA AGGACATGTG GTTTATCTGG CAAGACTGAG GCAAATAAGA 
CTACAAAATT TTAATGAGCG CCAACAGATT AAAGCCAAAC TTCGTGGTGA GAATAAAGAA 
GCTGGTGGTA CCAAAGGACA AGACGCAACT GAAGAGGCTG ACATGAGGCT CAAAAAGATG 
GAGTCACTTA AGGCCCAAGC AAATGCACGT GCTGCTGTAC TAAAAGAACA GCTAGAGCGA 
AAAAGAAAGG AAGCTTATGA AAGAGAAAAG AAAGTGTGGG AAGAACATTT GGTGGTGAGG 
GTAAAAAGCT CAGATGTCCC TCCATCTATG GAGCATCATG AAGCAGGTGG TTCTCCATCA 
CAGCAGCAGA TGAAACCTGT TATTTCTGTG ACTTCAGCTT TGAAAGAAGT GGGCCTGGAT 
GGAAGTTTAA CTGAGTCCCA AGAAACTTCA GAAGAAATGG AAAAGAGTAA CAATGCTATT 
TCAAGTAAGC GAGAAATACT GCGTAGGCTG AATGAAAATC TTAAAGCTCA AGAGGATGAA 
AAGGAAAAGC ATCATCACGC TGATTCTTGT GAGACCGTTG GTCACAGAGA TGAGAGAGAG 
TATGAGAAGG AAAATGCCAT TTCCTCTGAT CGCAAGAAAT GGGAGATGGG AGGTCAGCTT 
GTGATTCCTC TGGATGAAGT GACACTGGAT GCATCCTTCT CTGCTTCTGA AAAACATACT 
GTGGGAGAGG TTATTAAGTT AGATTCTAGT GGCTCTCCAA GAAAAGTCTG GGGGAAAAGC 
CCTACAGATT CTGTGCTGAA GATACTTGGA GAAGCTGAAT TACAGCTACA GACAGAACTA 
CTAGAAAACA CGCCTTTTAA AAGTGAGGTT TGTGTTGAAG AGGAGAACTA CAAACCCCTA 
CTCACTGGAG AAGAGAATCT GCAGTGTATT TCAAAAGAAG TAAACCCATC AGCCACTGTT 
GATACTGAAA CAAACAGTCC AAAGTTTGCT AAGGTGTCTC CAGCAATGTC CGAAGGAAAT 
GTGGAAGAAC CTGATGATTT GGAAACAGAA GTTCTACAAG AGCCAAGTGC CATACCCTCA 
GATGGGAGTT TGCCGCCTGT TGTTAATGAG GTGTGGACCA GAGCTAAGGC AACTGGGTTG 
GAGGATAGCA TTGCTGTGCA GCAGAATGAC GTTTGTGAAG AGCGAATTCC AGGGACTGTG 
GACCAGTCCT GTAAGGATCA TATAGAACCT GCAGGAAACG ATTCTCCGCA GTCTGGCTGT 
GATGAAGAGA AGTCAGTACA GCCAGAATTG TTTTTCCAGA AAGTGGTTCA CTCCAAGGAC 
TTGAACTTAG TTCAGTCAGC TCATCGTTCC CCAGAAGAAG CAATTCCAAT TCGATCTCAC 
TCTGATTCTC CACCAAAAGC TAAAAGCAAG AATTCCCTAC TGATTGGACT TTCCACTGGT 
CTGTTTGATG CAAACAACCC AAAGATGCTG AGGACCTGTT CACTTCCAGA CCTTTCCAAG 
CTCTTCAGAA CCCTAATGGA TGTTCCCACT GTGGGAGACG TTCATCAAGA CAGTCTTGAA 
ATAGAGGAGC TTGAGGATGA ACATATTAAA GAAGGGCCCT CGGATTGCGA GGACATTGTA 
TTTGAAGAAA CTGACACAGA TTTACAGGAG CTTCAGGCCT CAATGGAGCA GCTGCTTAGG 
GAGCAACCCG GTGACGAATA CAGTGAGGAG GAAGAGTCTG TCTTAAAAAG CAGCAATGTG 
GAGCGGGCAG CAAGAGGGAC AGATGTCCCA GATGAGGAGG ACAACCCCAG CAGTGAAAGC 
GCCCTAAACG AGGAATGGCA CTCAGATAAC AGTGATGCTG AGACTACTAG TGAGTGTGAA 
TATGACAGTG TCTTTAACCA TTTAGAGGAA CTAAGACTTC ACTTGGAACA AGAAATGGGC 
TTTGAAAAGT TCTTTGAGGT CTATGAGAAA GTAAAGGCTA TTCATGAGGA TGAAGATGAA 
AATATTGAAA TTTCTTCAAC CATAGTCGAG AACATCTTGG GCACTGAGCA TCAGCACCTT 
TATGCCAAGA TTCTGCATCT AGTCATGGCA GACGGAGCCT ATCAGGAAGA TAATGATGAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Analysis of a cAMP regulated coactivator family reveals an alternative phosphorylation motif for AMPK family members.
PloS one12(2)e0173013(2017)
Sonntag T,Moresco JJ,Vaughan JM,Matsumura S,Yates JR 3rd,Montminy M

NEK1 and DYNC2H1 are both involved in short rib polydactyly Majewski type but not in Beemer Langer cases.
Journal of medical genetics49(4)227-33(2012 Apr)
El Hokayem J,Huber C,Couv? A,Aziza J,Baujat G,Bouvier R,Cavalcanti DP,Collins FA,Cordier MP,Delezoide AL,Gonzales M,Johnson D,Le Merrer M,Levy-Mozziconacci A,Loget P,Martin-Coignard D,Martinovic J,Mortier GR,Perez MJ,Roume J,Scarano G,Munnich A,Cormier-Daire V

Novel asymmetrically localizing components of human centrosomes identified by complementary proteomics methods.
The EMBO journal30(8)1520-35(2011 Apr)
Jakobsen L,Vanselow K,Skogs M,Toyoda Y,Lundberg E,Poser I,Falkenby LG,Bennetzen M,Westendorf J,Nigg EA,Uhlen M,Hyman AA,Andersen JS

NEK1 mutations cause short-rib polydactyly syndrome type majewski.
American journal of human genetics88(1)106-14(2011 Jan)
Thiel C,Kessler K,Giessl A,Dimmler A,Shalev SA,von der Haar S,Zenker M,Zahnleiter D,St?ss H,Beinder E,Abou Jamra R,Ekici AB,Schr?der-Kress N,Aigner T,Kirchner T,Reis A,Brandst?tter JH,Rauch A

The NIMA-related kinase NEK1 cycles through the nucleus.
Biochemical and biophysical research communications389(1)52-6(2009 Nov)
Hilton LK,White MC,Quarmby LM

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Nek1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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