nek1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	nek1
Entrez Gene ID	100036653
Full Name	NIMA-related kinase 1
Synonyms	srps2
General protein information	Preferred Names NIMA-related kinase 1 Names serine/threonine-protein kinase Nek1 NIMA (never in mitosis gene a)-related kinase 1
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012952724.2	XP_012808178.1	serine/threonine-protein kinase Nek1 isoform X2
XM_012952724.3	XP_012808178.2	serine/threonine-protein kinase Nek1 isoform X2
XM_031890277.1	XP_031746137.1	serine/threonine-protein kinase Nek1 isoform X3
XM_012971595.3	XP_012827049.2	serine/threonine-protein kinase Nek1 isoform X1
XM_012971595.2	XP_012827049.1	serine/threonine-protein kinase Nek1 isoform X1
NM_001097211.1	NP_001090680.1	serine/threonine-protein kinase Nek1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	NEK1	XP_003586402.2
Rattus norvegicus (Norway rat)	Nek1	NP_001099552.2
Gallus gallus (chicken)	NEK1	XP_420401.3
Homo sapiens (human)	NEK1	NP_001186326.1
	NEK1	XP_001083275.1
Canis lupus familiaris (dog)	NEK1	XP_543184.2
Mus musculus (house mouse)	Nek1	NP_780298.2
Pan troglodytes (chimpanzee)	NEK1	XP_526727.2
Danio rerio (zebrafish)	nek1	XP_005160601.1
Xenopus tropicalis (tropical clawed frog)	nek1	NP_001090680.1

Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following nek1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nek1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa47348	XM_012971595.3 Latest version!	Xenopus tropicalis NIMA-related kinase 1 (nek1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa47349	XM_012952724.3 Latest version!	Xenopus tropicalis NIMA-related kinase 1 (nek1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa47350	XM_031890277.1 Latest version!	Xenopus tropicalis NIMA-related kinase 1 (nek1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa22622	NM_001097211.1 Latest version!	Xenopus tropicalis NIMA-related kinase 1 (nek1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OXa29842	XM_012971595.2 Latest version!	Xenopus tropicalis NIMA-related kinase 1 (nek1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OXa29843	XM_012952724.2 Latest version!	Xenopus tropicalis NIMA-related kinase 1 (nek1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nek1 ( XM_012971595.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012827049.2 Xenopus tropicalis NIMA-related kinase 1 (nek1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa47348
Clone ID Related Accession (Same CDS sequence)	XM_012971595.3
Accession Version	XM_012971595.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3963bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	serine/threonine-protein kinase Nek1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012971595.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATAAGT ACGTGAGAGT CAGCAAGATC GGAGAAGGTT CCTTTGGAAA GGCCATTCTG 
GTGAAATCTA AAGATGATGG CAAGCAATAT GTCATCAAAG AAATCGGCAT CTCCAAGATG 
TCAAACAAGG AGAGGGAGGA ATCCAGGAGG GAAGTTGCTG TGTTGGCAAA TATGAAGCAT 
CCAAATATTG TCCAATACCA AGAGTCATTT GAAGAAAGTG GATGTCTTTA CATTGTGATG 
GATTATTGTG AAGGAGGGGA CCTATTTAAA AGAATAAATT CACAGAAAGG GGTTTTGTTT 
TCAGAAGATC AGATCCTGGA CTGGTTTGTG CAGCTGTGCC TGGCTCTAAA GCATGTACAT 
GACAGGAAGA TTCTGCATCG GGACATAAAG TCCCAGAATA TCTTTCTAAC AAAAGGTGGT 
ACAATACAAC TGGGTGATTT TGGAATAGCA CGGGTGTTAA ACAGCACTGT AGAGCTGGCT 
CGCACCTGTA TAGGAACTCC GTACTATCTG TCTCCTGAGA TATGTGAAAA CAGGCCATAT 
AACAACAAGA GTGATATTTG GGCGCTGGGC TGTGTTTTGT ATGAAATGTG CACCCTCAAG 
CATGCGTTTG AAGCTGGCAA CATGAAGAAC TTAGTACTGA AAATAATCCG AGGCTCCTAT 
CCCCCTGTGT CTGTGCATTA TTCTTATGAC CTACGCAATC TGCTCTCAAT GCTTTTTAAG 
AGGAATCCTA GGGATCGTCC ATCTGTCAAC TCCATATTGG AGAAGCCTTT TATATATAGA 
CGTATTGAGA AGTTTTTGTC CCCACAGCAT ATTGCTGAAG AGTTTGGCCT GGGAGCCTTT 
GTGAATCACC CCAAACCTGG GATCCAAGCT GCAGCAGCCA AAAGACCAAC TCCAGCACAA 
GTGGTTCCTG CTTCCCCTCC TGCTCACAAA ATCACTAAAC CTGCTGCTAA ATACGGAGTG 
CCTTTAACAA CAAAAAAATC TTCAGATGCT GCAAAGAAGC ATCATGATAA GAAACCCCAA 
ATCAGACACA GACAGGCCCC AGCAGTACCA CTGAAGAAAG TGAATCCTGT AGACGAAAGG 
AGGAAAATGT TTGAGGACGG GGCCAAGAAA AAAAGGATGG ATGTTAGACA ACTGGAGAAG 
GAAAAAAGAC AACGGGAACA GTTTAATCTA CTAAGGGCCG AACAGATGAG GAGACTGGAA 
AAGGAAAGGT TGGAGCGAAT AAATCGTGCA CGAGAGCAAG GCTGGAGAAA TGTAATCAGC 
TCAGGAGGAA GCGGAGATAT TAAGGCACCT TATTTTGGTG GAGGGGGAGG TGTAGCACCC 
CCACAAGGCA TGGGTAGAGG TCCCTATGAG CATTACCATT CCATTTTTGA TCAGATGCAG 
CAGCAGAAGG AAAACATCAA AGCAGCAGTG GAAAAAGAGG TGAAACTAAA GGCAGATATC 
CAAAATCCTG ACACTTATAG CAGAGTTCCT AAGCATCCTG AACAAGAAAA TTGTCCTTCA 
GCTCATTCTC CATTCCCAGA TGGTGATGCA GTGAGAAGGG AACTGAAGAG GCTGCAAGAA 
GTACACAATC AAGCCATTGG CAACAAGATT CAAGGACACG TTTCTGCAGA AAAGGCAAGA 
CAGGTTGAAG AGTTTTTGCA ACGGAAGCAT GAAGCAATGC AAAATAAGGC TCGAGCAGAG 
GGACATATGG ACGCAGTGCA CAACCTGGCA GCTATCTGTG GAGGCAGGCC AAATTCTGCA 
GGAGGAGGCA AGGCACGCAA CAGAGAAGAA AAGGAGTATT TAGCAAGACT TAGACAAATC 
AGACTCCAGA ATTTTAATGA GCGACAACAA ATAAAAGCCA AGCTGAGAGG GGCAAAGGGG 
GATGAAGGTG ACGCAGATGG TAAGGAAAGC AGTGAAGACG CTGAACTAAG AAGAAAGAAA 
ATTGAAGCTT TGAAGGCTCA AGCAAATGCA CGAGCTGCTT TAATAAAAGA AGAACTTGAG 
AGAAAGAGAA GAGAAGCAGA TAAAAGAGAG AAGAGACTAT GGGAAGAGCA TCTGGCGTTA 
AAAAATCCTA ATGTGCCTTG TAATGCAGTT GGGGCAGCAC CTGGTTCCCC CAAACATTCA 
CATCTTGACG TTGGGGGATC ACCTGTCCGA GGGCATCCTC ATGCGAAACC CAGCACACCT 
GCCATATCTA TGACTTCAGC GCTAAGGAAT GTTGGGGCTG AAGGCGAAGA TGCAGGTCTC 
AGGGATCCAA CAGAGGGCTC CAAAACATCA GACACCTCAG TACAAAATGA AAAGCGGCAA 
ATACTCCGCC GATTAAATAA TAACATGAAA TCTCAGGAAG AAACAGAAGC CACAAACCCG 
CCAACAGAGG AAAATGGAGT GCAAACCAAT GGCGAGGAAC AGCAAGAGAG TGGTGAGCCT 
CTGGCAGTAG ACAGAAAGAA GTGGCAGGCC GAGCCTCCTC CTGTTATCCC TCTTTCCCAG 
CTCACACTTG TAGAGGATAC AGTGTTCCCA TTGGACAATC TAACAGTTGG AGATGTCATC 
AAACTGGATG TGGAAGAACC CCAGAGACGA CTGTGGGGGA AAGATCCTGG TGATTCTGTG 
TTAAAGGTAC TCGGTGAGGC CAAACTCCAG CTTCAAACAG AATTGCTTCA AGTAACAATC 
GATGAACAGT GCCATGAAGA AGCAGATGAA GAGAAGGAAA ACCCTGACAT GCGGGACAAT 
TTGTTAAATA AAGTTTCACC CACTGAGGGA AAGGCTTTAA TTTTGCCTAA TAGAGAGGGA 
ATGCCACAGC CAGAAGTGTC TCCACTTGTA GAAGAGGAAT CCTCCGTGCC CCTTGTTGCC 
TTTAACCAAG TAGAGATCCA GTCTAATGAA GATCCTGAAG ATTTGGAGAC TGAGATACTT 
GAAGAAACAG AAGGCAAGGA GAAAGAGGGC AATGAGTGCC CAGTCCACGT TACAAATGTT 
TGGACTGAGC AGACCAAACA AGAAAGTGAG GGACAGGACG CTGTTGAATG CGAGCAACCA 
AATGAAGATG CCAAACTGGA TACTGAATGC TCAGAACAAG AGCAAAATAT AGAGAATCAG 
TCAAATAGAG AGTCAAGTGT ACCCAAAGCT TTATTTGAAA AGGTGCCACT CCCTCAAAGT 
GTAGTCACTC CAACATTCTC TGCCCAGTCA TCCTTCGATG ATTCCTTACC GCCTCGTTCT 
CGATCAGCTT CTCCAACCAA AACCAAAGGC AAATCAGCAC TGCTGATTGG ACTCTCTGCT 
GGTTTATTTG ATGCAAACAA CCCAAAGATG CTGCGCACAT GTTCGTTGCC CGACCTTAGC 
AAATTATTCA AGACCTTTGT GGATGAGCCC ACAGTGGCTG ATGTAGAAGG GGAGGAGCAG 
TTGGAAATAG AAGATATAGA GGATGAACCT GTTCAGGATG CGGATCACTC TGATTCAGAG 
GACACCATGT TTGGGGAGAC AGACCGAGAT TTACAGGAAA TGCAGGCATC AATGGAACAA 
CTATTGAGAG AGGAGCCTAC GGAAGATTAC TGCGCAGAGA AAGCAACCCA TTTAAACTCA 
GAACCACCAG CAGGAATCAA AGAGGAAAAT ATTGAGTCTG CCATTATTAA TGGGGCAGAG 
GAGGAGGAGG AGGAAGATGA AGAAGAAGAA GATGGAAATC AGCCAAGTGG TGAAAGTGTC 
ATTAATGAAG AATGGCATTC GGATGATAGC AGTGAGGACT TTGCCAGTGA ATGTGATGAT 
GGTGATAGCC TTTTTGCCCA TCTGGAAGAA ATGAGGTTTA AACTGGAACA AGAGATTGGT 
TTTGAAAAAT TCATAGAAGT CTACAATAAA ATCAAGGCTA TACACGAAGA TGAGGATGAA 
AATATTGATA GTTGTTCAAC AGTCGTGGAG AATATTCTAG GCTGTGAGCA CCAGCATTTG 
TATCCCAGAG TCCTTCGCCT AGTGATGGCA GATGGAGCTT ATCAAGAAGA TAATGACGAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012827049.2
CDS	179..4141
Translation	MNKYVRVSKIGEGSFGKAILVKSKDDGKQYVIKEIGISKMSNKEREESRREVAVLANMKHPNIVQYQESFEESGCLYIVMDYCEGGDLFKRINSQKGVLFSEDQILDWFVQLCLALKHVHDRKILHRDIKSQNIFLTKGGTIQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENRPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIIRGSYPPVSVHYSYDLRNLLSMLFKRNPRDRPSVNSILEKPFIYRRIEKFLSPQHIAEEFGLGAFVNHPKPGIQAAAAKRPTPAQVVPASPPAHKITKPAAKYGVPLTTKKSSDAAKKHHDKKPQIRHRQAPAVPLKKVNPVDERRKMFEDGAKKKRMDVRQLEKEKRQREQFNLLRAEQMRRLEKERLERINRAREQGWRNVISSGGSGDIKAPYFGGGGGVAPPQGMGRGPYEHYHSIFDQMQQQKENIKAAVEKEVKLKADIQNPDTYSRVPKHPEQENCPSAHSPFPDGDAVRRELKRLQEVHNQAIGNKIQGHVSAEKARQVEEFLQRKHEAMQNKARAEGHMDAVHNLAAICGGRPNSAGGGKARNREEKEYLARLRQIRLQNFNERQQIKAKLRGAKGDEGDADGKESSEDAELRRKKIEALKAQANARAALIKEELERKRREADKREKRLWEEHLALKNPNVPCNAVGAAPGSPKHSHLDVGGSPVRGHPHAKPSTPAISMTSALRNVGAEGEDAGLRDPTEGSKTSDTSVQNEKRQILRRLNNNMKSQEETEATNPPTEENGVQTNGEEQQESGEPLAVDRKKWQAEPPPVIPLSQLTLVEDTVFPLDNLTVGDVIKLDVEEPQRRLWGKDPGDSVLKVLGEAKLQLQTELLQVTIDEQCHEEADEEKENPDMRDNLLNKVSPTEGKALILPNREGMPQPEVSPLVEEESSVPLVAFNQVEIQSNEDPEDLETEILEETEGKEKEGNECPVHVTNVWTEQTKQESEGQDAVECEQPNEDAKLDTECSEQEQNIENQSNRESSVPKALFEKVPLPQSVVTPTFSAQSSFDDSLPPRSRSASPTKTKGKSALLIGLSAGLFDANNPKMLRTCSLPDLSKLFKTFVDEPTVADVEGEEQLEIEDIEDEPVQDADHSDSEDTMFGETDRDLQEMQASMEQLLREEPTEDYCAEKATHLNSEPPAGIKEENIESAIINGAEEEEEEDEEEEDGNQPSGESVINEEWHSDDSSEDFASECDDGDSLFAHLEEMRFKLEQEIGFEKFIEVYNKIKAIHEDEDENIDSCSTVVENILGCEHQHLYPRVLRLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_012971595.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis NIMA-related kinase 1 (nek1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012971595.3

ORF Insert Sequence:

ATGAATAAGT ACGTGAGAGT CAGCAAGATC GGAGAAGGTT CCTTTGGAAA GGCCATTCTG 
GTGAAATCTA AAGATGATGG CAAGCAATAT GTCATCAAAG AAATCGGCAT CTCCAAGATG 
TCAAACAAGG AGAGGGAGGA ATCCAGGAGG GAAGTTGCTG TGTTGGCAAA TATGAAGCAT 
CCAAATATTG TCCAATACCA AGAGTCATTT GAAGAAAGTG GATGTCTTTA CATTGTGATG 
GATTATTGTG AAGGAGGGGA CCTATTTAAA AGAATAAATT CACAGAAAGG GGTTTTGTTT 
TCAGAAGATC AGATCCTGGA CTGGTTTGTG CAGCTGTGCC TGGCTCTAAA GCATGTACAT 
GACAGGAAGA TTCTGCATCG GGACATAAAG TCCCAGAATA TCTTTCTAAC AAAAGGTGGT 
ACAATACAAC TGGGTGATTT TGGAATAGCA CGGGTGTTAA ACAGCACTGT AGAGCTGGCT 
CGCACCTGTA TAGGAACTCC GTACTATCTG TCTCCTGAGA TATGTGAAAA CAGGCCATAT 
AACAACAAGA GTGATATTTG GGCGCTGGGC TGTGTTTTGT ATGAAATGTG CACCCTCAAG 
CATGCGTTTG AAGCTGGCAA CATGAAGAAC TTAGTACTGA AAATAATCCG AGGCTCCTAT 
CCCCCTGTGT CTGTGCATTA TTCTTATGAC CTACGCAATC TGCTCTCAAT GCTTTTTAAG 
AGGAATCCTA GGGATCGTCC ATCTGTCAAC TCCATATTGG AGAAGCCTTT TATATATAGA 
CGTATTGAGA AGTTTTTGTC CCCACAGCAT ATTGCTGAAG AGTTTGGCCT GGGAGCCTTT 
GTGAATCACC CCAAACCTGG GATCCAAGCT GCAGCAGCCA AAAGACCAAC TCCAGCACAA 
GTGGTTCCTG CTTCCCCTCC TGCTCACAAA ATCACTAAAC CTGCTGCTAA ATACGGAGTG 
CCTTTAACAA CAAAAAAATC TTCAGATGCT GCAAAGAAGC ATCATGATAA GAAACCCCAA 
ATCAGACACA GACAGGCCCC AGCAGTACCA CTGAAGAAAG TGAATCCTGT AGACGAAAGG 
AGGAAAATGT TTGAGGACGG GGCCAAGAAA AAAAGGATGG ATGTTAGACA ACTGGAGAAG 
GAAAAAAGAC AACGGGAACA GTTTAATCTA CTAAGGGCCG AACAGATGAG GAGACTGGAA 
AAGGAAAGGT TGGAGCGAAT AAATCGTGCA CGAGAGCAAG GCTGGAGAAA TGTAATCAGC 
TCAGGAGGAA GCGGAGATAT TAAGGCACCT TATTTTGGTG GAGGGGGAGG TGTAGCACCC 
CCACAAGGCA TGGGTAGAGG TCCCTATGAG CATTACCATT CCATTTTTGA TCAGATGCAG 
CAGCAGAAGG AAAACATCAA AGCAGCAGTG GAAAAAGAGG TGAAACTAAA GGCAGATATC 
CAAAATCCTG ACACTTATAG CAGAGTTCCT AAGCATCCTG AACAAGAAAA TTGTCCTTCA 
GCTCATTCTC CATTCCCAGA TGGTGATGCA GTGAGAAGGG AACTGAAGAG GCTGCAAGAA 
GTACACAATC AAGCCATTGG CAACAAGATT CAAGGACACG TTTCTGCAGA AAAGGCAAGA 
CAGGTTGAAG AGTTTTTGCA ACGGAAGCAT GAAGCAATGC AAAATAAGGC TCGAGCAGAG 
GGACATATGG ACGCAGTGCA CAACCTGGCA GCTATCTGTG GAGGCAGGCC AAATTCTGCA 
GGAGGAGGCA AGGCACGCAA CAGAGAAGAA AAGGAGTATT TAGCAAGACT TAGACAAATC 
AGACTCCAGA ATTTTAATGA GCGACAACAA ATAAAAGCCA AGCTGAGAGG GGCAAAGGGG 
GATGAAGGTG ACGCAGATGG TAAGGAAAGC AGTGAAGACG CTGAACTAAG AAGAAAGAAA 
ATTGAAGCTT TGAAGGCTCA AGCAAATGCA CGAGCTGCTT TAATAAAAGA AGAACTTGAG 
AGAAAGAGAA GAGAAGCAGA TAAAAGAGAG AAGAGACTAT GGGAAGAGCA TCTGGCGTTA 
AAAAATCCTA ATGTGCCTTG TAATGCAGTT GGGGCAGCAC CTGGTTCCCC CAAACATTCA 
CATCTTGACG TTGGGGGATC ACCTGTCCGA GGGCATCCTC ATGCGAAACC CAGCACACCT 
GCCATATCTA TGACTTCAGC GCTAAGGAAT GTTGGGGCTG AAGGCGAAGA TGCAGGTCTC 
AGGGATCCAA CAGAGGGCTC CAAAACATCA GACACCTCAG TACAAAATGA AAAGCGGCAA 
ATACTCCGCC GATTAAATAA TAACATGAAA TCTCAGGAAG AAACAGAAGC CACAAACCCG 
CCAACAGAGG AAAATGGAGT GCAAACCAAT GGCGAGGAAC AGCAAGAGAG TGGTGAGCCT 
CTGGCAGTAG ACAGAAAGAA GTGGCAGGCC GAGCCTCCTC CTGTTATCCC TCTTTCCCAG 
CTCACACTTG TAGAGGATAC AGTGTTCCCA TTGGACAATC TAACAGTTGG AGATGTCATC 
AAACTGGATG TGGAAGAACC CCAGAGACGA CTGTGGGGGA AAGATCCTGG TGATTCTGTG 
TTAAAGGTAC TCGGTGAGGC CAAACTCCAG CTTCAAACAG AATTGCTTCA AGTAACAATC 
GATGAACAGT GCCATGAAGA AGCAGATGAA GAGAAGGAAA ACCCTGACAT GCGGGACAAT 
TTGTTAAATA AAGTTTCACC CACTGAGGGA AAGGCTTTAA TTTTGCCTAA TAGAGAGGGA 
ATGCCACAGC CAGAAGTGTC TCCACTTGTA GAAGAGGAAT CCTCCGTGCC CCTTGTTGCC 
TTTAACCAAG TAGAGATCCA GTCTAATGAA GATCCTGAAG ATTTGGAGAC TGAGATACTT 
GAAGAAACAG AAGGCAAGGA GAAAGAGGGC AATGAGTGCC CAGTCCACGT TACAAATGTT 
TGGACTGAGC AGACCAAACA AGAAAGTGAG GGACAGGACG CTGTTGAATG CGAGCAACCA 
AATGAAGATG CCAAACTGGA TACTGAATGC TCAGAACAAG AGCAAAATAT AGAGAATCAG 
TCAAATAGAG AGTCAAGTGT ACCCAAAGCT TTATTTGAAA AGGTGCCACT CCCTCAAAGT 
GTAGTCACTC CAACATTCTC TGCCCAGTCA TCCTTCGATG ATTCCTTACC GCCTCGTTCT 
CGATCAGCTT CTCCAACCAA AACCAAAGGC AAATCAGCAC TGCTGATTGG ACTCTCTGCT 
GGTTTATTTG ATGCAAACAA CCCAAAGATG CTGCGCACAT GTTCGTTGCC CGACCTTAGC 
AAATTATTCA AGACCTTTGT GGATGAGCCC ACAGTGGCTG ATGTAGAAGG GGAGGAGCAG 
TTGGAAATAG AAGATATAGA GGATGAACCT GTTCAGGATG CGGATCACTC TGATTCAGAG 
GACACCATGT TTGGGGAGAC AGACCGAGAT TTACAGGAAA TGCAGGCATC AATGGAACAA 
CTATTGAGAG AGGAGCCTAC GGAAGATTAC TGCGCAGAGA AAGCAACCCA TTTAAACTCA 
GAACCACCAG CAGGAATCAA AGAGGAAAAT ATTGAGTCTG CCATTATTAA TGGGGCAGAG 
GAGGAGGAGG AGGAAGATGA AGAAGAAGAA GATGGAAATC AGCCAAGTGG TGAAAGTGTC 
ATTAATGAAG AATGGCATTC GGATGATAGC AGTGAGGACT TTGCCAGTGA ATGTGATGAT 
GGTGATAGCC TTTTTGCCCA TCTGGAAGAA ATGAGGTTTA AACTGGAACA AGAGATTGGT 
TTTGAAAAAT TCATAGAAGT CTACAATAAA ATCAAGGCTA TACACGAAGA TGAGGATGAA 
AATATTGATA GTTGTTCAAC AGTCGTGGAG AATATTCTAG GCTGTGAGCA CCAGCATTTG 
TATCCCAGAG TCCTTCGCCT AGTGATGGCA GATGGAGCTT ATCAAGAAGA TAATGACGAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nek1 ( XM_012952724.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012808178.2 Xenopus tropicalis NIMA-related kinase 1 (nek1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa47349
Clone ID Related Accession (Same CDS sequence)	XM_012952724.3
Accession Version	XM_012952724.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3903bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	serine/threonine-protein kinase Nek1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012952724.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATAAGT ACGTGAGAGT CAGCAAGATC GGAGAAGGTT CCTTTGGAAA GGCCATTCTG 
GTGAAATCTA AAGATGATGG CAAGCAATAT GTCATCAAAG AAATCGGCAT CTCCAAGATG 
TCAAACAAGG AGAGGGAGGA ATCCAGGAGG GAAGTTGCTG TGTTGGCAAA TATGAAGCAT 
CCAAATATTG TCCAATACCA AGAGTCATTT GAAGAAAGTG GATGTCTTTA CATTGTGATG 
GATTATTGTG AAGGAGGGGA CCTATTTAAA AGAATAAATT CACAGAAAGG GGTTTTGTTT 
TCAGAAGATC AGATCCTGGA CTGGTTTGTG CAGCTGTGCC TGGCTCTAAA GCATGTACAT 
GACAGGAAGA TTCTGCATCG GGACATAAAG TCCCAGAATA TCTTTCTAAC AAAAGGTGGT 
ACAATACAAC TGGGTGATTT TGGAATAGCA CGGGTGTTAA ACAGCACTGT AGAGCTGGCT 
CGCACCTGTA TAGGAACTCC GTACTATCTG TCTCCTGAGA TATGTGAAAA CAGGCCATAT 
AACAACAAGA GTGATATTTG GGCGCTGGGC TGTGTTTTGT ATGAAATGTG CACCCTCAAG 
CATGCGTTTG AAGCTGGCAA CATGAAGAAC TTAGTACTGA AAATAATCCG AGGCTCCTAT 
CCCCCTGTGT CTGTGCATTA TTCTTATGAC CTACGCAATC TGCTCTCAAT GCTTTTTAAG 
AGGAATCCTA GGGATCGTCC ATCTGTCAAC TCCATATTGG AGAAGCCTTT TATATATAGA 
CGTATTGAGA AGTTTTTGTC CCCACAGCAT ATTGCTGAAG AGTTTGGCCT GGGAGCCTTT 
GTGAATCACC CCAAACCTGG GATCCAAGCT GCAGCAGCCA AAAGACCAAC TCCAGCACAA 
GTGGTTCCTG CTTCCCCTCC TGCTCACAAA ATCACTAAAC CTGCTGCTAA ATACGGAGTG 
CCTTTAACAA CAAAAAAATC TTCAGATGCT GCAAAGAAGC ATCATGATAA GAAACCCCAA 
ATCAGACACA GACAGGACGG GGCCAAGAAA AAAAGGATGG ATGTTAGACA ACTGGAGAAG 
GAAAAAAGAC AACGGGAACA GTTTAATCTA CTAAGGGCCG AACAGATGAG GAGACTGGAA 
AAGGAAAGGT TGGAGCGAAT AAATCGTGCA CGAGAGCAAG GCTGGAGAAA TGTAATCAGC 
TCAGGAGGAA GCGGAGATAT TAAGGCACCT TATTTTGGTG GAGGGGGAGG TGTAGCACCC 
CCACAAGGCA TGGGTAGAGG TCCCTATGAG CATTACCATT CCATTTTTGA TCAGATGCAG 
CAGCAGAAGG AAAACATCAA AGCAGCAGTG GAAAAAGAGG TGAAACTAAA GGCAGATATC 
CAAAATCCTG ACACTTATAG CAGAGTTCCT AAGCATCCTG AACAAGAAAA TTGTCCTTCA 
GCTCATTCTC CATTCCCAGA TGGTGATGCA GTGAGAAGGG AACTGAAGAG GCTGCAAGAA 
GTACACAATC AAGCCATTGG CAACAAGATT CAAGGACACG TTTCTGCAGA AAAGGCAAGA 
CAGGTTGAAG AGTTTTTGCA ACGGAAGCAT GAAGCAATGC AAAATAAGGC TCGAGCAGAG 
GGACATATGG ACGCAGTGCA CAACCTGGCA GCTATCTGTG GAGGCAGGCC AAATTCTGCA 
GGAGGAGGCA AGGCACGCAA CAGAGAAGAA AAGGAGTATT TAGCAAGACT TAGACAAATC 
AGACTCCAGA ATTTTAATGA GCGACAACAA ATAAAAGCCA AGCTGAGAGG GGCAAAGGGG 
GATGAAGGTG ACGCAGATGG TAAGGAAAGC AGTGAAGACG CTGAACTAAG AAGAAAGAAA 
ATTGAAGCTT TGAAGGCTCA AGCAAATGCA CGAGCTGCTT TAATAAAAGA AGAACTTGAG 
AGAAAGAGAA GAGAAGCAGA TAAAAGAGAG AAGAGACTAT GGGAAGAGCA TCTGGCGTTA 
AAAAATCCTA ATGTGCCTTG TAATGCAGTT GGGGCAGCAC CTGGTTCCCC CAAACATTCA 
CATCTTGACG TTGGGGGATC ACCTGTCCGA GGGCATCCTC ATGCGAAACC CAGCACACCT 
GCCATATCTA TGACTTCAGC GCTAAGGAAT GTTGGGGCTG AAGGCGAAGA TGCAGGTCTC 
AGGGATCCAA CAGAGGGCTC CAAAACATCA GACACCTCAG TACAAAATGA AAAGCGGCAA 
ATACTCCGCC GATTAAATAA TAACATGAAA TCTCAGGAAG AAACAGAAGC CACAAACCCG 
CCAACAGAGG AAAATGGAGT GCAAACCAAT GGCGAGGAAC AGCAAGAGAG TGGTGAGCCT 
CTGGCAGTAG ACAGAAAGAA GTGGCAGGCC GAGCCTCCTC CTGTTATCCC TCTTTCCCAG 
CTCACACTTG TAGAGGATAC AGTGTTCCCA TTGGACAATC TAACAGTTGG AGATGTCATC 
AAACTGGATG TGGAAGAACC CCAGAGACGA CTGTGGGGGA AAGATCCTGG TGATTCTGTG 
TTAAAGGTAC TCGGTGAGGC CAAACTCCAG CTTCAAACAG AATTGCTTCA AGTAACAATC 
GATGAACAGT GCCATGAAGA AGCAGATGAA GAGAAGGAAA ACCCTGACAT GCGGGACAAT 
TTGTTAAATA AAGTTTCACC CACTGAGGGA AAGGCTTTAA TTTTGCCTAA TAGAGAGGGA 
ATGCCACAGC CAGAAGTGTC TCCACTTGTA GAAGAGGAAT CCTCCGTGCC CCTTGTTGCC 
TTTAACCAAG TAGAGATCCA GTCTAATGAA GATCCTGAAG ATTTGGAGAC TGAGATACTT 
GAAGAAACAG AAGGCAAGGA GAAAGAGGGC AATGAGTGCC CAGTCCACGT TACAAATGTT 
TGGACTGAGC AGACCAAACA AGAAAGTGAG GGACAGGACG CTGTTGAATG CGAGCAACCA 
AATGAAGATG CCAAACTGGA TACTGAATGC TCAGAACAAG AGCAAAATAT AGAGAATCAG 
TCAAATAGAG AGTCAAGTGT ACCCAAAGCT TTATTTGAAA AGGTGCCACT CCCTCAAAGT 
GTAGTCACTC CAACATTCTC TGCCCAGTCA TCCTTCGATG ATTCCTTACC GCCTCGTTCT 
CGATCAGCTT CTCCAACCAA AACCAAAGGC AAATCAGCAC TGCTGATTGG ACTCTCTGCT 
GGTTTATTTG ATGCAAACAA CCCAAAGATG CTGCGCACAT GTTCGTTGCC CGACCTTAGC 
AAATTATTCA AGACCTTTGT GGATGAGCCC ACAGTGGCTG ATGTAGAAGG GGAGGAGCAG 
TTGGAAATAG AAGATATAGA GGATGAACCT GTTCAGGATG CGGATCACTC TGATTCAGAG 
GACACCATGT TTGGGGAGAC AGACCGAGAT TTACAGGAAA TGCAGGCATC AATGGAACAA 
CTATTGAGAG AGGAGCCTAC GGAAGATTAC TGCGCAGAGA AAGCAACCCA TTTAAACTCA 
GAACCACCAG CAGGAATCAA AGAGGAAAAT ATTGAGTCTG CCATTATTAA TGGGGCAGAG 
GAGGAGGAGG AGGAAGATGA AGAAGAAGAA GATGGAAATC AGCCAAGTGG TGAAAGTGTC 
ATTAATGAAG AATGGCATTC GGATGATAGC AGTGAGGACT TTGCCAGTGA ATGTGATGAT 
GGTGATAGCC TTTTTGCCCA TCTGGAAGAA ATGAGGTTTA AACTGGAACA AGAGATTGGT 
TTTGAAAAAT TCATAGAAGT CTACAATAAA ATCAAGGCTA TACACGAAGA TGAGGATGAA 
AATATTGATA GTTGTTCAAC AGTCGTGGAG AATATTCTAG GCTGTGAGCA CCAGCATTTG 
TATCCCAGAG TCCTTCGCCT AGTGATGGCA GATGGAGCTT ATCAAGAAGA TAATGACGAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012808178.2
CDS	179..4081
Translation	MNKYVRVSKIGEGSFGKAILVKSKDDGKQYVIKEIGISKMSNKEREESRREVAVLANMKHPNIVQYQESFEESGCLYIVMDYCEGGDLFKRINSQKGVLFSEDQILDWFVQLCLALKHVHDRKILHRDIKSQNIFLTKGGTIQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENRPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIIRGSYPPVSVHYSYDLRNLLSMLFKRNPRDRPSVNSILEKPFIYRRIEKFLSPQHIAEEFGLGAFVNHPKPGIQAAAAKRPTPAQVVPASPPAHKITKPAAKYGVPLTTKKSSDAAKKHHDKKPQIRHRQDGAKKKRMDVRQLEKEKRQREQFNLLRAEQMRRLEKERLERINRAREQGWRNVISSGGSGDIKAPYFGGGGGVAPPQGMGRGPYEHYHSIFDQMQQQKENIKAAVEKEVKLKADIQNPDTYSRVPKHPEQENCPSAHSPFPDGDAVRRELKRLQEVHNQAIGNKIQGHVSAEKARQVEEFLQRKHEAMQNKARAEGHMDAVHNLAAICGGRPNSAGGGKARNREEKEYLARLRQIRLQNFNERQQIKAKLRGAKGDEGDADGKESSEDAELRRKKIEALKAQANARAALIKEELERKRREADKREKRLWEEHLALKNPNVPCNAVGAAPGSPKHSHLDVGGSPVRGHPHAKPSTPAISMTSALRNVGAEGEDAGLRDPTEGSKTSDTSVQNEKRQILRRLNNNMKSQEETEATNPPTEENGVQTNGEEQQESGEPLAVDRKKWQAEPPPVIPLSQLTLVEDTVFPLDNLTVGDVIKLDVEEPQRRLWGKDPGDSVLKVLGEAKLQLQTELLQVTIDEQCHEEADEEKENPDMRDNLLNKVSPTEGKALILPNREGMPQPEVSPLVEEESSVPLVAFNQVEIQSNEDPEDLETEILEETEGKEKEGNECPVHVTNVWTEQTKQESEGQDAVECEQPNEDAKLDTECSEQEQNIENQSNRESSVPKALFEKVPLPQSVVTPTFSAQSSFDDSLPPRSRSASPTKTKGKSALLIGLSAGLFDANNPKMLRTCSLPDLSKLFKTFVDEPTVADVEGEEQLEIEDIEDEPVQDADHSDSEDTMFGETDRDLQEMQASMEQLLREEPTEDYCAEKATHLNSEPPAGIKEENIESAIINGAEEEEEEDEEEEDGNQPSGESVINEEWHSDDSSEDFASECDDGDSLFAHLEEMRFKLEQEIGFEKFIEVYNKIKAIHEDEDENIDSCSTVVENILGCEHQHLYPRVLRLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_012952724.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis NIMA-related kinase 1 (nek1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012952724.3

ORF Insert Sequence:

ATGAATAAGT ACGTGAGAGT CAGCAAGATC GGAGAAGGTT CCTTTGGAAA GGCCATTCTG 
GTGAAATCTA AAGATGATGG CAAGCAATAT GTCATCAAAG AAATCGGCAT CTCCAAGATG 
TCAAACAAGG AGAGGGAGGA ATCCAGGAGG GAAGTTGCTG TGTTGGCAAA TATGAAGCAT 
CCAAATATTG TCCAATACCA AGAGTCATTT GAAGAAAGTG GATGTCTTTA CATTGTGATG 
GATTATTGTG AAGGAGGGGA CCTATTTAAA AGAATAAATT CACAGAAAGG GGTTTTGTTT 
TCAGAAGATC AGATCCTGGA CTGGTTTGTG CAGCTGTGCC TGGCTCTAAA GCATGTACAT 
GACAGGAAGA TTCTGCATCG GGACATAAAG TCCCAGAATA TCTTTCTAAC AAAAGGTGGT 
ACAATACAAC TGGGTGATTT TGGAATAGCA CGGGTGTTAA ACAGCACTGT AGAGCTGGCT 
CGCACCTGTA TAGGAACTCC GTACTATCTG TCTCCTGAGA TATGTGAAAA CAGGCCATAT 
AACAACAAGA GTGATATTTG GGCGCTGGGC TGTGTTTTGT ATGAAATGTG CACCCTCAAG 
CATGCGTTTG AAGCTGGCAA CATGAAGAAC TTAGTACTGA AAATAATCCG AGGCTCCTAT 
CCCCCTGTGT CTGTGCATTA TTCTTATGAC CTACGCAATC TGCTCTCAAT GCTTTTTAAG 
AGGAATCCTA GGGATCGTCC ATCTGTCAAC TCCATATTGG AGAAGCCTTT TATATATAGA 
CGTATTGAGA AGTTTTTGTC CCCACAGCAT ATTGCTGAAG AGTTTGGCCT GGGAGCCTTT 
GTGAATCACC CCAAACCTGG GATCCAAGCT GCAGCAGCCA AAAGACCAAC TCCAGCACAA 
GTGGTTCCTG CTTCCCCTCC TGCTCACAAA ATCACTAAAC CTGCTGCTAA ATACGGAGTG 
CCTTTAACAA CAAAAAAATC TTCAGATGCT GCAAAGAAGC ATCATGATAA GAAACCCCAA 
ATCAGACACA GACAGGACGG GGCCAAGAAA AAAAGGATGG ATGTTAGACA ACTGGAGAAG 
GAAAAAAGAC AACGGGAACA GTTTAATCTA CTAAGGGCCG AACAGATGAG GAGACTGGAA 
AAGGAAAGGT TGGAGCGAAT AAATCGTGCA CGAGAGCAAG GCTGGAGAAA TGTAATCAGC 
TCAGGAGGAA GCGGAGATAT TAAGGCACCT TATTTTGGTG GAGGGGGAGG TGTAGCACCC 
CCACAAGGCA TGGGTAGAGG TCCCTATGAG CATTACCATT CCATTTTTGA TCAGATGCAG 
CAGCAGAAGG AAAACATCAA AGCAGCAGTG GAAAAAGAGG TGAAACTAAA GGCAGATATC 
CAAAATCCTG ACACTTATAG CAGAGTTCCT AAGCATCCTG AACAAGAAAA TTGTCCTTCA 
GCTCATTCTC CATTCCCAGA TGGTGATGCA GTGAGAAGGG AACTGAAGAG GCTGCAAGAA 
GTACACAATC AAGCCATTGG CAACAAGATT CAAGGACACG TTTCTGCAGA AAAGGCAAGA 
CAGGTTGAAG AGTTTTTGCA ACGGAAGCAT GAAGCAATGC AAAATAAGGC TCGAGCAGAG 
GGACATATGG ACGCAGTGCA CAACCTGGCA GCTATCTGTG GAGGCAGGCC AAATTCTGCA 
GGAGGAGGCA AGGCACGCAA CAGAGAAGAA AAGGAGTATT TAGCAAGACT TAGACAAATC 
AGACTCCAGA ATTTTAATGA GCGACAACAA ATAAAAGCCA AGCTGAGAGG GGCAAAGGGG 
GATGAAGGTG ACGCAGATGG TAAGGAAAGC AGTGAAGACG CTGAACTAAG AAGAAAGAAA 
ATTGAAGCTT TGAAGGCTCA AGCAAATGCA CGAGCTGCTT TAATAAAAGA AGAACTTGAG 
AGAAAGAGAA GAGAAGCAGA TAAAAGAGAG AAGAGACTAT GGGAAGAGCA TCTGGCGTTA 
AAAAATCCTA ATGTGCCTTG TAATGCAGTT GGGGCAGCAC CTGGTTCCCC CAAACATTCA 
CATCTTGACG TTGGGGGATC ACCTGTCCGA GGGCATCCTC ATGCGAAACC CAGCACACCT 
GCCATATCTA TGACTTCAGC GCTAAGGAAT GTTGGGGCTG AAGGCGAAGA TGCAGGTCTC 
AGGGATCCAA CAGAGGGCTC CAAAACATCA GACACCTCAG TACAAAATGA AAAGCGGCAA 
ATACTCCGCC GATTAAATAA TAACATGAAA TCTCAGGAAG AAACAGAAGC CACAAACCCG 
CCAACAGAGG AAAATGGAGT GCAAACCAAT GGCGAGGAAC AGCAAGAGAG TGGTGAGCCT 
CTGGCAGTAG ACAGAAAGAA GTGGCAGGCC GAGCCTCCTC CTGTTATCCC TCTTTCCCAG 
CTCACACTTG TAGAGGATAC AGTGTTCCCA TTGGACAATC TAACAGTTGG AGATGTCATC 
AAACTGGATG TGGAAGAACC CCAGAGACGA CTGTGGGGGA AAGATCCTGG TGATTCTGTG 
TTAAAGGTAC TCGGTGAGGC CAAACTCCAG CTTCAAACAG AATTGCTTCA AGTAACAATC 
GATGAACAGT GCCATGAAGA AGCAGATGAA GAGAAGGAAA ACCCTGACAT GCGGGACAAT 
TTGTTAAATA AAGTTTCACC CACTGAGGGA AAGGCTTTAA TTTTGCCTAA TAGAGAGGGA 
ATGCCACAGC CAGAAGTGTC TCCACTTGTA GAAGAGGAAT CCTCCGTGCC CCTTGTTGCC 
TTTAACCAAG TAGAGATCCA GTCTAATGAA GATCCTGAAG ATTTGGAGAC TGAGATACTT 
GAAGAAACAG AAGGCAAGGA GAAAGAGGGC AATGAGTGCC CAGTCCACGT TACAAATGTT 
TGGACTGAGC AGACCAAACA AGAAAGTGAG GGACAGGACG CTGTTGAATG CGAGCAACCA 
AATGAAGATG CCAAACTGGA TACTGAATGC TCAGAACAAG AGCAAAATAT AGAGAATCAG 
TCAAATAGAG AGTCAAGTGT ACCCAAAGCT TTATTTGAAA AGGTGCCACT CCCTCAAAGT 
GTAGTCACTC CAACATTCTC TGCCCAGTCA TCCTTCGATG ATTCCTTACC GCCTCGTTCT 
CGATCAGCTT CTCCAACCAA AACCAAAGGC AAATCAGCAC TGCTGATTGG ACTCTCTGCT 
GGTTTATTTG ATGCAAACAA CCCAAAGATG CTGCGCACAT GTTCGTTGCC CGACCTTAGC 
AAATTATTCA AGACCTTTGT GGATGAGCCC ACAGTGGCTG ATGTAGAAGG GGAGGAGCAG 
TTGGAAATAG AAGATATAGA GGATGAACCT GTTCAGGATG CGGATCACTC TGATTCAGAG 
GACACCATGT TTGGGGAGAC AGACCGAGAT TTACAGGAAA TGCAGGCATC AATGGAACAA 
CTATTGAGAG AGGAGCCTAC GGAAGATTAC TGCGCAGAGA AAGCAACCCA TTTAAACTCA 
GAACCACCAG CAGGAATCAA AGAGGAAAAT ATTGAGTCTG CCATTATTAA TGGGGCAGAG 
GAGGAGGAGG AGGAAGATGA AGAAGAAGAA GATGGAAATC AGCCAAGTGG TGAAAGTGTC 
ATTAATGAAG AATGGCATTC GGATGATAGC AGTGAGGACT TTGCCAGTGA ATGTGATGAT 
GGTGATAGCC TTTTTGCCCA TCTGGAAGAA ATGAGGTTTA AACTGGAACA AGAGATTGGT 
TTTGAAAAAT TCATAGAAGT CTACAATAAA ATCAAGGCTA TACACGAAGA TGAGGATGAA 
AATATTGATA GTTGTTCAAC AGTCGTGGAG AATATTCTAG GCTGTGAGCA CCAGCATTTG 
TATCCCAGAG TCCTTCGCCT AGTGATGGCA GATGGAGCTT ATCAAGAAGA TAATGACGAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nek1 ( XM_031890277.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031746137.1 Xenopus tropicalis NIMA-related kinase 1 (nek1), transcript variant X3, mRNA.

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CloneID	OXa47350
Clone ID Related Accession (Same CDS sequence)	XM_031890277.1
Accession Version	XM_031890277.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3873bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	serine/threonine-protein kinase Nek1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATAAGT ACGTGAGAGT CAGCAAGATC GGAGAAGGTT CCTTTGGAAA GGCCATTCTG 
GTGAAATCTA AAGATGATGG CAAGCAATAT GTCATCAAAG AAATCGGCAT CTCCAAGATG 
TCAAACAAGG AGAGGGAGGA ATCCAGGAGG GAAGTTGCTG TGTTGGCAAA TATGAAGCAT 
CCAAATATTG TCCAATACCA AGAGTCATTT GAAGAAAGTG GATGTCTTTA CATTGTGATG 
GATTATTGTG AAGGAGGGGA CCTATTTAAA AGAATAAATT CACAGAAAGG GGTTTTGTTT 
TCAGAAGATC AGATCCTGGA CTGGTTTGTG CAGCTGTGCC TGGCTCTAAA GCATGTACAT 
GACAGGAAGA TTCTGCATCG GGACATAAAG TCCCAGAATA TCTTTCTAAC AAAAGGTGGT 
ACAATACAAC TGGGTGATTT TGGAATAGCA CGGGTGTTAA ACAGCACTGT AGAGCTGGCT 
CGCACCTGTA TAGGAACTCC GTACTATCTG TCTCCTGAGA TATGTGAAAA CAGGCCATAT 
AACAACAAGA GTGATATTTG GGCGCTGGGC TGTGTTTTGT ATGAAATGTG CACCCTCAAG 
CATGCGTTTG AAGCTGGCAA CATGAAGAAC TTAGTACTGA AAATAATCCG AGGCTCCTAT 
CCCCCTGTGT CTGTGCATTA TTCTTATGAC CTACGCAATC TGCTCTCAAT GCTTTTTAAG 
AGGAATCCTA GGGATCGTCC ATCTGTCAAC TCCATATTGG AGAAGCCTTT TATATATAGA 
CGTATTGAGA AGTTTTTGTC CCCACAGCAT ATTGCTGAAG AGTTTGGCCT GGGAGCCTTT 
GTGAATCACC CCAAACCTGG GATCCAAGCT GCAGCAGCCA AAAGACCAAC TCCAGCACAA 
GTGGTTCCTG CTTCCCCTCC TGCTCACAAA ATCACTAAAC CTGCTGCTAA ATACGGAGTG 
CCTTTAACAA CAAAAAAATC TTCAGATGCT GCAAAGAAGC ATCATGATAA GAAACCCCAA 
ATCAGACACA GACAGGCCCC AGCAGTACCA CTGAAGAAAG TGAATCCTGT AGACGAAAGG 
AGGAAAATGT TTGAGGACGG GGCCAAGAAA AAAAGGATGG ATGTTAGACA ACTGGAGAAG 
GAAAAAAGAC AACGGGAACA GTTTAATCTA CTAAGGGCCG AACAGATGAG GAGACTGGAA 
AAGGAAAGGT TGGAGCGAAT AAATCGTGCA CGAGAGCAAG GCTGGAGAAA TGTAATCAGC 
TCAGGAGGAA GCGGAGATAT TAAGATGCAG CAGCAGAAGG AAAACATCAA AGCAGCAGTG 
GAAAAAGAGG TGAAACTAAA GGCAGATATC CAAAATCCTG ACACTTATAG CAGAGTTCCT 
AAGCATCCTG AACAAGAAAA TTGTCCTTCA GCTCATTCTC CATTCCCAGA TGGTGATGCA 
GTGAGAAGGG AACTGAAGAG GCTGCAAGAA GTACACAATC AAGCCATTGG CAACAAGATT 
CAAGGACACG TTTCTGCAGA AAAGGCAAGA CAGGTTGAAG AGTTTTTGCA ACGGAAGCAT 
GAAGCAATGC AAAATAAGGC TCGAGCAGAG GGACATATGG ACGCAGTGCA CAACCTGGCA 
GCTATCTGTG GAGGCAGGCC AAATTCTGCA GGAGGAGGCA AGGCACGCAA CAGAGAAGAA 
AAGGAGTATT TAGCAAGACT TAGACAAATC AGACTCCAGA ATTTTAATGA GCGACAACAA 
ATAAAAGCCA AGCTGAGAGG GGCAAAGGGG GATGAAGGTG ACGCAGATGG TAAGGAAAGC 
AGTGAAGACG CTGAACTAAG AAGAAAGAAA ATTGAAGCTT TGAAGGCTCA AGCAAATGCA 
CGAGCTGCTT TAATAAAAGA AGAACTTGAG AGAAAGAGAA GAGAAGCAGA TAAAAGAGAG 
AAGAGACTAT GGGAAGAGCA TCTGGCGTTA AAAAATCCTA ATGTGCCTTG TAATGCAGTT 
GGGGCAGCAC CTGGTTCCCC CAAACATTCA CATCTTGACG TTGGGGGATC ACCTGTCCGA 
GGGCATCCTC ATGCGAAACC CAGCACACCT GCCATATCTA TGACTTCAGC GCTAAGGAAT 
GTTGGGGCTG AAGGCGAAGA TGCAGGTCTC AGGGATCCAA CAGAGGGCTC CAAAACATCA 
GACACCTCAG TACAAAATGA AAAGCGGCAA ATACTCCGCC GATTAAATAA TAACATGAAA 
TCTCAGGAAG AAACAGAAGC CACAAACCCG CCAACAGAGG AAAATGGAGT GCAAACCAAT 
GGCGAGGAAC AGCAAGAGAG TGGTGAGCCT CTGGCAGTAG ACAGAAAGAA GTGGCAGGCC 
GAGCCTCCTC CTGTTATCCC TCTTTCCCAG CTCACACTTG TAGAGGATAC AGTGTTCCCA 
TTGGACAATC TAACAGTTGG AGATGTCATC AAACTGGATG TGGAAGAACC CCAGAGACGA 
CTGTGGGGGA AAGATCCTGG TGATTCTGTG TTAAAGGTAC TCGGTGAGGC CAAACTCCAG 
CTTCAAACAG AATTGCTTCA AGTAACAATC GATGAACAGT GCCATGAAGA AGCAGATGAA 
GAGAAGGAAA ACCCTGACAT GCGGGACAAT TTGTTAAATA AAGTTTCACC CACTGAGGGA 
AAGGCTTTAA TTTTGCCTAA TAGAGAGGGA ATGCCACAGC CAGAAGTGTC TCCACTTGTA 
GAAGAGGAAT CCTCCGTGCC CCTTGTTGCC TTTAACCAAG TAGAGATCCA GTCTAATGAA 
GATCCTGAAG ATTTGGAGAC TGAGATACTT GAAGAAACAG AAGGCAAGGA GAAAGAGGGC 
AATGAGTGCC CAGTCCACGT TACAAATGTT TGGACTGAGC AGACCAAACA AGAAAGTGAG 
GGACAGGACG CTGTTGAATG CGAGCAACCA AATGAAGATG CCAAACTGGA TACTGAATGC 
TCAGAACAAG AGCAAAATAT AGAGAATCAG TCAAATAGAG AGTCAAGTGT ACCCAAAGCT 
TTATTTGAAA AGGTGCCACT CCCTCAAAGT GTAGTCACTC CAACATTCTC TGCCCAGTCA 
TCCTTCGATG ATTCCTTACC GCCTCGTTCT CGATCAGCTT CTCCAACCAA AACCAAAGGC 
AAATCAGCAC TGCTGATTGG ACTCTCTGCT GGTTTATTTG ATGCAAACAA CCCAAAGATG 
CTGCGCACAT GTTCGTTGCC CGACCTTAGC AAATTATTCA AGACCTTTGT GGATGAGCCC 
ACAGTGGCTG ATGTAGAAGG GGAGGAGCAG TTGGAAATAG AAGATATAGA GGATGAACCT 
GTTCAGGATG CGGATCACTC TGATTCAGAG GACACCATGT TTGGGGAGAC AGACCGAGAT 
TTACAGGAAA TGCAGGCATC AATGGAACAA CTATTGAGAG AGGAGCCTAC GGAAGATTAC 
TGCGCAGAGA AAGCAACCCA TTTAAACTCA GAACCACCAG CAGGAATCAA AGAGGAAAAT 
ATTGAGTCTG CCATTATTAA TGGGGCAGAG GAGGAGGAGG AGGAAGATGA AGAAGAAGAA 
GATGGAAATC AGCCAAGTGG TGAAAGTGTC ATTAATGAAG AATGGCATTC GGATGATAGC 
AGTGAGGACT TTGCCAGTGA ATGTGATGAT GGTGATAGCC TTTTTGCCCA TCTGGAAGAA 
ATGAGGTTTA AACTGGAACA AGAGATTGGT TTTGAAAAAT TCATAGAAGT CTACAATAAA 
ATCAAGGCTA TACACGAAGA TGAGGATGAA AATATTGATA GTTGTTCAAC AGTCGTGGAG 
AATATTCTAG GCTGTGAGCA CCAGCATTTG TATCCCAGAG TCCTTCGCCT AGTGATGGCA 
GATGGAGCTT ATCAAGAAGA TAATGACGAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031746137.1
CDS	179..4051
Translation	MNKYVRVSKIGEGSFGKAILVKSKDDGKQYVIKEIGISKMSNKEREESRREVAVLANMKHPNIVQYQESFEESGCLYIVMDYCEGGDLFKRINSQKGVLFSEDQILDWFVQLCLALKHVHDRKILHRDIKSQNIFLTKGGTIQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENRPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIIRGSYPPVSVHYSYDLRNLLSMLFKRNPRDRPSVNSILEKPFIYRRIEKFLSPQHIAEEFGLGAFVNHPKPGIQAAAAKRPTPAQVVPASPPAHKITKPAAKYGVPLTTKKSSDAAKKHHDKKPQIRHRQAPAVPLKKVNPVDERRKMFEDGAKKKRMDVRQLEKEKRQREQFNLLRAEQMRRLEKERLERINRAREQGWRNVISSGGSGDIKMQQQKENIKAAVEKEVKLKADIQNPDTYSRVPKHPEQENCPSAHSPFPDGDAVRRELKRLQEVHNQAIGNKIQGHVSAEKARQVEEFLQRKHEAMQNKARAEGHMDAVHNLAAICGGRPNSAGGGKARNREEKEYLARLRQIRLQNFNERQQIKAKLRGAKGDEGDADGKESSEDAELRRKKIEALKAQANARAALIKEELERKRREADKREKRLWEEHLALKNPNVPCNAVGAAPGSPKHSHLDVGGSPVRGHPHAKPSTPAISMTSALRNVGAEGEDAGLRDPTEGSKTSDTSVQNEKRQILRRLNNNMKSQEETEATNPPTEENGVQTNGEEQQESGEPLAVDRKKWQAEPPPVIPLSQLTLVEDTVFPLDNLTVGDVIKLDVEEPQRRLWGKDPGDSVLKVLGEAKLQLQTELLQVTIDEQCHEEADEEKENPDMRDNLLNKVSPTEGKALILPNREGMPQPEVSPLVEEESSVPLVAFNQVEIQSNEDPEDLETEILEETEGKEKEGNECPVHVTNVWTEQTKQESEGQDAVECEQPNEDAKLDTECSEQEQNIENQSNRESSVPKALFEKVPLPQSVVTPTFSAQSSFDDSLPPRSRSASPTKTKGKSALLIGLSAGLFDANNPKMLRTCSLPDLSKLFKTFVDEPTVADVEGEEQLEIEDIEDEPVQDADHSDSEDTMFGETDRDLQEMQASMEQLLREEPTEDYCAEKATHLNSEPPAGIKEENIESAIINGAEEEEEEDEEEEDGNQPSGESVINEEWHSDDSSEDFASECDDGDSLFAHLEEMRFKLEQEIGFEKFIEVYNKIKAIHEDEDENIDSCSTVVENILGCEHQHLYPRVLRLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_031890277.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis NIMA-related kinase 1 (nek1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031890277.1

ORF Insert Sequence:

ATGAATAAGT ACGTGAGAGT CAGCAAGATC GGAGAAGGTT CCTTTGGAAA GGCCATTCTG 
GTGAAATCTA AAGATGATGG CAAGCAATAT GTCATCAAAG AAATCGGCAT CTCCAAGATG 
TCAAACAAGG AGAGGGAGGA ATCCAGGAGG GAAGTTGCTG TGTTGGCAAA TATGAAGCAT 
CCAAATATTG TCCAATACCA AGAGTCATTT GAAGAAAGTG GATGTCTTTA CATTGTGATG 
GATTATTGTG AAGGAGGGGA CCTATTTAAA AGAATAAATT CACAGAAAGG GGTTTTGTTT 
TCAGAAGATC AGATCCTGGA CTGGTTTGTG CAGCTGTGCC TGGCTCTAAA GCATGTACAT 
GACAGGAAGA TTCTGCATCG GGACATAAAG TCCCAGAATA TCTTTCTAAC AAAAGGTGGT 
ACAATACAAC TGGGTGATTT TGGAATAGCA CGGGTGTTAA ACAGCACTGT AGAGCTGGCT 
CGCACCTGTA TAGGAACTCC GTACTATCTG TCTCCTGAGA TATGTGAAAA CAGGCCATAT 
AACAACAAGA GTGATATTTG GGCGCTGGGC TGTGTTTTGT ATGAAATGTG CACCCTCAAG 
CATGCGTTTG AAGCTGGCAA CATGAAGAAC TTAGTACTGA AAATAATCCG AGGCTCCTAT 
CCCCCTGTGT CTGTGCATTA TTCTTATGAC CTACGCAATC TGCTCTCAAT GCTTTTTAAG 
AGGAATCCTA GGGATCGTCC ATCTGTCAAC TCCATATTGG AGAAGCCTTT TATATATAGA 
CGTATTGAGA AGTTTTTGTC CCCACAGCAT ATTGCTGAAG AGTTTGGCCT GGGAGCCTTT 
GTGAATCACC CCAAACCTGG GATCCAAGCT GCAGCAGCCA AAAGACCAAC TCCAGCACAA 
GTGGTTCCTG CTTCCCCTCC TGCTCACAAA ATCACTAAAC CTGCTGCTAA ATACGGAGTG 
CCTTTAACAA CAAAAAAATC TTCAGATGCT GCAAAGAAGC ATCATGATAA GAAACCCCAA 
ATCAGACACA GACAGGCCCC AGCAGTACCA CTGAAGAAAG TGAATCCTGT AGACGAAAGG 
AGGAAAATGT TTGAGGACGG GGCCAAGAAA AAAAGGATGG ATGTTAGACA ACTGGAGAAG 
GAAAAAAGAC AACGGGAACA GTTTAATCTA CTAAGGGCCG AACAGATGAG GAGACTGGAA 
AAGGAAAGGT TGGAGCGAAT AAATCGTGCA CGAGAGCAAG GCTGGAGAAA TGTAATCAGC 
TCAGGAGGAA GCGGAGATAT TAAGATGCAG CAGCAGAAGG AAAACATCAA AGCAGCAGTG 
GAAAAAGAGG TGAAACTAAA GGCAGATATC CAAAATCCTG ACACTTATAG CAGAGTTCCT 
AAGCATCCTG AACAAGAAAA TTGTCCTTCA GCTCATTCTC CATTCCCAGA TGGTGATGCA 
GTGAGAAGGG AACTGAAGAG GCTGCAAGAA GTACACAATC AAGCCATTGG CAACAAGATT 
CAAGGACACG TTTCTGCAGA AAAGGCAAGA CAGGTTGAAG AGTTTTTGCA ACGGAAGCAT 
GAAGCAATGC AAAATAAGGC TCGAGCAGAG GGACATATGG ACGCAGTGCA CAACCTGGCA 
GCTATCTGTG GAGGCAGGCC AAATTCTGCA GGAGGAGGCA AGGCACGCAA CAGAGAAGAA 
AAGGAGTATT TAGCAAGACT TAGACAAATC AGACTCCAGA ATTTTAATGA GCGACAACAA 
ATAAAAGCCA AGCTGAGAGG GGCAAAGGGG GATGAAGGTG ACGCAGATGG TAAGGAAAGC 
AGTGAAGACG CTGAACTAAG AAGAAAGAAA ATTGAAGCTT TGAAGGCTCA AGCAAATGCA 
CGAGCTGCTT TAATAAAAGA AGAACTTGAG AGAAAGAGAA GAGAAGCAGA TAAAAGAGAG 
AAGAGACTAT GGGAAGAGCA TCTGGCGTTA AAAAATCCTA ATGTGCCTTG TAATGCAGTT 
GGGGCAGCAC CTGGTTCCCC CAAACATTCA CATCTTGACG TTGGGGGATC ACCTGTCCGA 
GGGCATCCTC ATGCGAAACC CAGCACACCT GCCATATCTA TGACTTCAGC GCTAAGGAAT 
GTTGGGGCTG AAGGCGAAGA TGCAGGTCTC AGGGATCCAA CAGAGGGCTC CAAAACATCA 
GACACCTCAG TACAAAATGA AAAGCGGCAA ATACTCCGCC GATTAAATAA TAACATGAAA 
TCTCAGGAAG AAACAGAAGC CACAAACCCG CCAACAGAGG AAAATGGAGT GCAAACCAAT 
GGCGAGGAAC AGCAAGAGAG TGGTGAGCCT CTGGCAGTAG ACAGAAAGAA GTGGCAGGCC 
GAGCCTCCTC CTGTTATCCC TCTTTCCCAG CTCACACTTG TAGAGGATAC AGTGTTCCCA 
TTGGACAATC TAACAGTTGG AGATGTCATC AAACTGGATG TGGAAGAACC CCAGAGACGA 
CTGTGGGGGA AAGATCCTGG TGATTCTGTG TTAAAGGTAC TCGGTGAGGC CAAACTCCAG 
CTTCAAACAG AATTGCTTCA AGTAACAATC GATGAACAGT GCCATGAAGA AGCAGATGAA 
GAGAAGGAAA ACCCTGACAT GCGGGACAAT TTGTTAAATA AAGTTTCACC CACTGAGGGA 
AAGGCTTTAA TTTTGCCTAA TAGAGAGGGA ATGCCACAGC CAGAAGTGTC TCCACTTGTA 
GAAGAGGAAT CCTCCGTGCC CCTTGTTGCC TTTAACCAAG TAGAGATCCA GTCTAATGAA 
GATCCTGAAG ATTTGGAGAC TGAGATACTT GAAGAAACAG AAGGCAAGGA GAAAGAGGGC 
AATGAGTGCC CAGTCCACGT TACAAATGTT TGGACTGAGC AGACCAAACA AGAAAGTGAG 
GGACAGGACG CTGTTGAATG CGAGCAACCA AATGAAGATG CCAAACTGGA TACTGAATGC 
TCAGAACAAG AGCAAAATAT AGAGAATCAG TCAAATAGAG AGTCAAGTGT ACCCAAAGCT 
TTATTTGAAA AGGTGCCACT CCCTCAAAGT GTAGTCACTC CAACATTCTC TGCCCAGTCA 
TCCTTCGATG ATTCCTTACC GCCTCGTTCT CGATCAGCTT CTCCAACCAA AACCAAAGGC 
AAATCAGCAC TGCTGATTGG ACTCTCTGCT GGTTTATTTG ATGCAAACAA CCCAAAGATG 
CTGCGCACAT GTTCGTTGCC CGACCTTAGC AAATTATTCA AGACCTTTGT GGATGAGCCC 
ACAGTGGCTG ATGTAGAAGG GGAGGAGCAG TTGGAAATAG AAGATATAGA GGATGAACCT 
GTTCAGGATG CGGATCACTC TGATTCAGAG GACACCATGT TTGGGGAGAC AGACCGAGAT 
TTACAGGAAA TGCAGGCATC AATGGAACAA CTATTGAGAG AGGAGCCTAC GGAAGATTAC 
TGCGCAGAGA AAGCAACCCA TTTAAACTCA GAACCACCAG CAGGAATCAA AGAGGAAAAT 
ATTGAGTCTG CCATTATTAA TGGGGCAGAG GAGGAGGAGG AGGAAGATGA AGAAGAAGAA 
GATGGAAATC AGCCAAGTGG TGAAAGTGTC ATTAATGAAG AATGGCATTC GGATGATAGC 
AGTGAGGACT TTGCCAGTGA ATGTGATGAT GGTGATAGCC TTTTTGCCCA TCTGGAAGAA 
ATGAGGTTTA AACTGGAACA AGAGATTGGT TTTGAAAAAT TCATAGAAGT CTACAATAAA 
ATCAAGGCTA TACACGAAGA TGAGGATGAA AATATTGATA GTTGTTCAAC AGTCGTGGAG 
AATATTCTAG GCTGTGAGCA CCAGCATTTG TATCCCAGAG TCCTTCGCCT AGTGATGGCA 
GATGGAGCTT ATCAAGAAGA TAATGACGAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nek1 ( NM_001097211.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001090680.1 Xenopus tropicalis NIMA-related kinase 1 (nek1), mRNA.

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CloneID	OXa22622
Clone ID Related Accession (Same CDS sequence)	NM_001097211.1
Accession Version	NM_001097211.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3879bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Xenopus tropicalis(tropical clawed frog)
Product	serine/threonine-protein kinase Nek1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC127335.1. ##Evidence-Data-START## Transcript exon combination :: BC127335.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00028290, SAMD00028291 [ECO:0000348] ##Evidence-Data-END##

ATGAATAAGT ACGTGAGAGT CAGCAAGATC GGAGAAGGTT CCTTTGGAAA GGCCATTCTG 
GTGAAATCTA AAGATGATGG CAAGCAATAT GTCATCAAAG AAATCGGCAT CTCCAAGATG 
TCAAACAAGG AGAGGGAGGA ATCCAGGAGG GAAGTTGCTG TGTTGGCAAA TATGAAGCAT 
CCAAATATTG TCCAATACCA AGAGTCATTT GAAGAAAGTG GATGTCTTTA CATTGTGATG 
GATTATTGTG AAGGAGGGGA CCTATTTAAA AGAATAAATT CACAGAAAGG GGTTTTGTTT 
TCAGAAGATC AGATCCTGGA CTGGTTTGTG CAGCTGTGCC TGGCTCTAAA GCATGTACAT 
GACAGGAAGA TTCTGCATCG GGACATAAAG TCCCAGAATA TCTTTCTAAC AAAAGGTGGT 
ACAATACAAC TGGGTGATTT TGGAATAGCA CGGGTGTTAA ACAGCACTGT AGAGCTGGCT 
CGCACCTGTA TAGGAACTCC GTACTATCTG TCTCCTGAGA TATGTGAAAA CAGGCCATAT 
AACAACAAGA GTGATATTTG GGCGCTGGGC TGTGTTTTGT ATGAAATGTG CACCCTCAAG 
CATGCGTTTG AAGCTGGCAA CATGAAGAAC TTAGTACTGA AAATAATCCG AGGCTCCTAT 
CCCCCTGTGT CTGTGCATTA TTCTTATGAC CTACGCAATC TGCTCTCAAT GCTTTTTAAG 
AGGAATCCTA GGGATCGTCC ATCTGTCAAC TCCATATTGG AGAAGCCTTT TATATATAGA 
CGTATTGAGA AGTTTTTGTC CCCACAGCAT ATTGCTGAAG AGTTTGGCCT GGGAGCCTTT 
GTGAATCACC CCAAACCTGG GATCCAAGCT GCAGCAGCCA AAAGACCAAC TCCAGCACAA 
GTGGTTCCTG CTTCCCCTCC TGCTCACAAA ATCACTAAAC CTGCTGCTAA ATACGGAGTG 
CCTTTAACAA CAAAAAAATC TTCAGATGCT GCAAAGAAGC ATCATGATAA GAAACCCCAA 
ATCAGACACA GACAGGCCCC AGCAGTACCA CTGAAGAAAG TGAATCCTGT AGACGAAAGG 
AGGAAAATGT TTGAGGACGG GGCCAAGAAA AAAAGGATGG ATGTTAGACA ACTGGAGAAG 
GAAAAAAGAC AACGGGAACA GTTTAATCTA CTAAGGGCCG AACAGATGAG GAGACTGGAA 
AAGGAAAGGT TGGAGCGAAT AAATCGTGCA CGAGAGCAAG GCTGGAGAAA TGTAATCAGC 
TCAGGAGGAA GCGGAGATAT TAAGGCACCT TATTTTGGTG GAGGGGGAGG TGTAGCACCC 
CCACAAGGCA TGGGTAGAGG TCCCTATGAG CATTACCATT CCATTTTTGA TCAGATGCAG 
CAGCAGAAGG AAAACATCAA AGCAGCAGTG GAAAAAGAGG TGAAACTAAA GGCAGATATC 
CAAAATCCTG ACACTTATAG CAGAGTTCCT AAGCATCCTG AACAAGAAAA TTGTCCTTCA 
GCTCATTCTC CATTCCCAGA TGGTGATGCA GTGAGAAGGG AACTGAAGAG GCTGCAAGAA 
GTACACAATC AAGCCATTGG CAACAAGATT CAAGGACACG TTTCTGCAGA AAAGGCAAGA 
CAGGTTGAAG AGTTTTTGCA ACGGAAGCAT GAAGCAATGC AAAATAAGGC TCGAGCAGAG 
GGACATATGG AGTATTTAGC AAGACTTAGA CAAATCAGAC TCCAGAATTT TAATGAGCGA 
CAACAAATAA AAGCCAAGCT GAGAGGGGCA AAGGGGGATG AAGGTGACGC AGATGGTAAG 
GAAAGCAGTG AAGACGCTGA ACTAAGAAGA AAGAAAATTG AAGCTTTGAA GGCTCAAGCA 
AATGCACGAG CTGCTTTAAT AAAAGAAGAA CTTGAGAGAA AGAGAAGAGA AGCAGATAAA 
AGAGAGAAGA GACTATGGGA AGAGCATCTG GCGTTAAAAA ATCCTAATGT GCCTTGTAAT 
GCAGTTGGGG CAGCACCTGG TTCCCCCAAA CATTCACATC TTGACGTTGG GGGATCACCT 
GTCCGAGGGC ATCCTCATGC GAAACCCAGC ACACCTGCCA TATCTATGAC TTCAGCGCTA 
AGGAATGTTG GGGCTGAAGG CGAAGATGCA GGTCTCAGGG ATCCAACAGA GGGCTCCAAA 
ACATCAGACA CCTCAGTACA AAATGAAAAG CGGCAAATAC TCCGCCGATT AAATAATAAC 
ATGAAATCTC AGGAAGAAAC AGAAGCCACA AACCCGCCAA CAGAGGAAAA TGGAGTGCAA 
ACCAATGGCG AGGAACAGCA AGAGAGTGGT GAGCCTCTGG CAGTAGACAG AAAGAAGTGG 
CAGGCCGAGC CTCCTCCTGT TATCCCTCTT TCCCAGCTCA CACTTGTAGA GGATACAGTG 
TTCCCATTGG ACAATCTAAC AGTTGGAGAT GTCATCAAAC TGGATGTGGA AGAACCCCAG 
AGACGACTGT GGGGGAAAGA TCCTGGTGAT TCTGTGTTAA AGGTACTCGG TGAGGCCAAA 
CTCCAGCTTC AAACAGAATT GCTTCAAGTA ACAATCGATG AACAGTGCCA TGAAGAAGCA 
GATGAAGAGA AGGAAAACCC TGACATGCGG GACAATTTGT TAAATAAAGT TTCACCCACT 
GAGGGAAAGG CTTTAATTTT GCCTAATAGA GAGGGAATGC CACAGCCAGA AGTGTCTCCA 
CTTGTAGAAG AGGAATCCTC CGTGCCCCTT GTTGCCTTTA ACCAAGTAGA GATCCAGTCT 
AATGAAGATC CTGAAGATTT GGAGACTGAG ATACTTGAAG AAACAGAAGG CAAGGAGAAA 
GAGGGCAATG AGTGCCCAGT CCACGTTACA AATGTTTGGA CTGAGCAGAC CAAACAAGAA 
AGTGAGGGAC AGGACGCTGT TGAATGCGAG CAACCAAATG AAGATGCCAA ACTGGATACT 
GAATGCTCAG AACAAGAGCA AAATATAGAG AATCAGTCAA ATAGAGAGTC AAGTGTACCC 
AAAGCTTTAT TTGAAAAGGT GCCACTCCCT CAAAGTGTAG TCACTCCAAC ATTCTCTGCC 
CAGTCATCCT TCGATGATTC CTTACCGCCT CGTTCTCGAT CAGCTTCTCC AACCAAAACC 
AAAGGCAAAT CAGCACTGCT GATTGGACTC TCTGCTGGTT TATTTGATGC AAACAACCCA 
AAGATGCTGC GCACATGTTC GTTGCCCGAC CTTAGCAAAT TATTCAAGAC CTTTGTGGAT 
GAGCCCACAG TGGCTGATGT AGAAGGGGAG GAGCAGTTGG AAATAGAAGA TATAGAGGAT 
GAACCTGTTC AGGATGCGGA TCACTCTGAT TCAGAGGACA CCATGTTTGG GGAGACAGAC 
CGAGATTTAC AGGAAATGCA GGCATCAATG GAACAACTAT TGAGAGAGGA GCCTACGGAA 
GATTACTGCG CAGAGAAAGC AACCCATTTA AACTCAGAAC CACCAGCAGG AATCAAAGAG 
GAAAATATTG AGTCTGCCAT TATTAATGGG GCAGAGGAGG AGGAGGAGGA AGATGAAGAA 
GAAGAAGATG GAAATCAGCC AAGTGGTGAA AGTGTCATTA ATGAAGAATG GCATTCGGAT 
GATAGCAGTG AGGACTTTGC CAGTGAATGT GATGATGGTG ATAGCCTTTT TGCCCATCTG 
GAAGAAATGA GGTTTAAACT GGAACAAGAG ATTGGTTTTG AAAAATTCAT AGAAGTCTAC 
AATAAAATCA AGGCTATACA CGAAGATGAG GATGAAAATA TTGATAGTTG TTCAACAGTC 
GTGGAGAATA TTCTAGGCTG TGAGCACCAG CATTTGTATC CCAGAGTCCT TCGCCTAGTG 
ATGGCAGATG GAGCTTATCA AGAAGATAAT GACGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001090680.1
CDS	185..4063
Translation	MNKYVRVSKIGEGSFGKAILVKSKDDGKQYVIKEIGISKMSNKEREESRREVAVLANMKHPNIVQYQESFEESGCLYIVMDYCEGGDLFKRINSQKGVLFSEDQILDWFVQLCLALKHVHDRKILHRDIKSQNIFLTKGGTIQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENRPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIIRGSYPPVSVHYSYDLRNLLSMLFKRNPRDRPSVNSILEKPFIYRRIEKFLSPQHIAEEFGLGAFVNHPKPGIQAAAAKRPTPAQVVPASPPAHKITKPAAKYGVPLTTKKSSDAAKKHHDKKPQIRHRQAPAVPLKKVNPVDERRKMFEDGAKKKRMDVRQLEKEKRQREQFNLLRAEQMRRLEKERLERINRAREQGWRNVISSGGSGDIKAPYFGGGGGVAPPQGMGRGPYEHYHSIFDQMQQQKENIKAAVEKEVKLKADIQNPDTYSRVPKHPEQENCPSAHSPFPDGDAVRRELKRLQEVHNQAIGNKIQGHVSAEKARQVEEFLQRKHEAMQNKARAEGHMEYLARLRQIRLQNFNERQQIKAKLRGAKGDEGDADGKESSEDAELRRKKIEALKAQANARAALIKEELERKRREADKREKRLWEEHLALKNPNVPCNAVGAAPGSPKHSHLDVGGSPVRGHPHAKPSTPAISMTSALRNVGAEGEDAGLRDPTEGSKTSDTSVQNEKRQILRRLNNNMKSQEETEATNPPTEENGVQTNGEEQQESGEPLAVDRKKWQAEPPPVIPLSQLTLVEDTVFPLDNLTVGDVIKLDVEEPQRRLWGKDPGDSVLKVLGEAKLQLQTELLQVTIDEQCHEEADEEKENPDMRDNLLNKVSPTEGKALILPNREGMPQPEVSPLVEEESSVPLVAFNQVEIQSNEDPEDLETEILEETEGKEKEGNECPVHVTNVWTEQTKQESEGQDAVECEQPNEDAKLDTECSEQEQNIENQSNRESSVPKALFEKVPLPQSVVTPTFSAQSSFDDSLPPRSRSASPTKTKGKSALLIGLSAGLFDANNPKMLRTCSLPDLSKLFKTFVDEPTVADVEGEEQLEIEDIEDEPVQDADHSDSEDTMFGETDRDLQEMQASMEQLLREEPTEDYCAEKATHLNSEPPAGIKEENIESAIINGAEEEEEEDEEEEDGNQPSGESVINEEWHSDDSSEDFASECDDGDSLFAHLEEMRFKLEQEIGFEKFIEVYNKIKAIHEDEDENIDSCSTVVENILGCEHQHLYPRVLRLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	NM_001097211.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis NIMA-related kinase 1 (nek1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001097211.1

ORF Insert Sequence:

ATGAATAAGT ACGTGAGAGT CAGCAAGATC GGAGAAGGTT CCTTTGGAAA GGCCATTCTG 
GTGAAATCTA AAGATGATGG CAAGCAATAT GTCATCAAAG AAATCGGCAT CTCCAAGATG 
TCAAACAAGG AGAGGGAGGA ATCCAGGAGG GAAGTTGCTG TGTTGGCAAA TATGAAGCAT 
CCAAATATTG TCCAATACCA AGAGTCATTT GAAGAAAGTG GATGTCTTTA CATTGTGATG 
GATTATTGTG AAGGAGGGGA CCTATTTAAA AGAATAAATT CACAGAAAGG GGTTTTGTTT 
TCAGAAGATC AGATCCTGGA CTGGTTTGTG CAGCTGTGCC TGGCTCTAAA GCATGTACAT 
GACAGGAAGA TTCTGCATCG GGACATAAAG TCCCAGAATA TCTTTCTAAC AAAAGGTGGT 
ACAATACAAC TGGGTGATTT TGGAATAGCA CGGGTGTTAA ACAGCACTGT AGAGCTGGCT 
CGCACCTGTA TAGGAACTCC GTACTATCTG TCTCCTGAGA TATGTGAAAA CAGGCCATAT 
AACAACAAGA GTGATATTTG GGCGCTGGGC TGTGTTTTGT ATGAAATGTG CACCCTCAAG 
CATGCGTTTG AAGCTGGCAA CATGAAGAAC TTAGTACTGA AAATAATCCG AGGCTCCTAT 
CCCCCTGTGT CTGTGCATTA TTCTTATGAC CTACGCAATC TGCTCTCAAT GCTTTTTAAG 
AGGAATCCTA GGGATCGTCC ATCTGTCAAC TCCATATTGG AGAAGCCTTT TATATATAGA 
CGTATTGAGA AGTTTTTGTC CCCACAGCAT ATTGCTGAAG AGTTTGGCCT GGGAGCCTTT 
GTGAATCACC CCAAACCTGG GATCCAAGCT GCAGCAGCCA AAAGACCAAC TCCAGCACAA 
GTGGTTCCTG CTTCCCCTCC TGCTCACAAA ATCACTAAAC CTGCTGCTAA ATACGGAGTG 
CCTTTAACAA CAAAAAAATC TTCAGATGCT GCAAAGAAGC ATCATGATAA GAAACCCCAA 
ATCAGACACA GACAGGCCCC AGCAGTACCA CTGAAGAAAG TGAATCCTGT AGACGAAAGG 
AGGAAAATGT TTGAGGACGG GGCCAAGAAA AAAAGGATGG ATGTTAGACA ACTGGAGAAG 
GAAAAAAGAC AACGGGAACA GTTTAATCTA CTAAGGGCCG AACAGATGAG GAGACTGGAA 
AAGGAAAGGT TGGAGCGAAT AAATCGTGCA CGAGAGCAAG GCTGGAGAAA TGTAATCAGC 
TCAGGAGGAA GCGGAGATAT TAAGGCACCT TATTTTGGTG GAGGGGGAGG TGTAGCACCC 
CCACAAGGCA TGGGTAGAGG TCCCTATGAG CATTACCATT CCATTTTTGA TCAGATGCAG 
CAGCAGAAGG AAAACATCAA AGCAGCAGTG GAAAAAGAGG TGAAACTAAA GGCAGATATC 
CAAAATCCTG ACACTTATAG CAGAGTTCCT AAGCATCCTG AACAAGAAAA TTGTCCTTCA 
GCTCATTCTC CATTCCCAGA TGGTGATGCA GTGAGAAGGG AACTGAAGAG GCTGCAAGAA 
GTACACAATC AAGCCATTGG CAACAAGATT CAAGGACACG TTTCTGCAGA AAAGGCAAGA 
CAGGTTGAAG AGTTTTTGCA ACGGAAGCAT GAAGCAATGC AAAATAAGGC TCGAGCAGAG 
GGACATATGG AGTATTTAGC AAGACTTAGA CAAATCAGAC TCCAGAATTT TAATGAGCGA 
CAACAAATAA AAGCCAAGCT GAGAGGGGCA AAGGGGGATG AAGGTGACGC AGATGGTAAG 
GAAAGCAGTG AAGACGCTGA ACTAAGAAGA AAGAAAATTG AAGCTTTGAA GGCTCAAGCA 
AATGCACGAG CTGCTTTAAT AAAAGAAGAA CTTGAGAGAA AGAGAAGAGA AGCAGATAAA 
AGAGAGAAGA GACTATGGGA AGAGCATCTG GCGTTAAAAA ATCCTAATGT GCCTTGTAAT 
GCAGTTGGGG CAGCACCTGG TTCCCCCAAA CATTCACATC TTGACGTTGG GGGATCACCT 
GTCCGAGGGC ATCCTCATGC GAAACCCAGC ACACCTGCCA TATCTATGAC TTCAGCGCTA 
AGGAATGTTG GGGCTGAAGG CGAAGATGCA GGTCTCAGGG ATCCAACAGA GGGCTCCAAA 
ACATCAGACA CCTCAGTACA AAATGAAAAG CGGCAAATAC TCCGCCGATT AAATAATAAC 
ATGAAATCTC AGGAAGAAAC AGAAGCCACA AACCCGCCAA CAGAGGAAAA TGGAGTGCAA 
ACCAATGGCG AGGAACAGCA AGAGAGTGGT GAGCCTCTGG CAGTAGACAG AAAGAAGTGG 
CAGGCCGAGC CTCCTCCTGT TATCCCTCTT TCCCAGCTCA CACTTGTAGA GGATACAGTG 
TTCCCATTGG ACAATCTAAC AGTTGGAGAT GTCATCAAAC TGGATGTGGA AGAACCCCAG 
AGACGACTGT GGGGGAAAGA TCCTGGTGAT TCTGTGTTAA AGGTACTCGG TGAGGCCAAA 
CTCCAGCTTC AAACAGAATT GCTTCAAGTA ACAATCGATG AACAGTGCCA TGAAGAAGCA 
GATGAAGAGA AGGAAAACCC TGACATGCGG GACAATTTGT TAAATAAAGT TTCACCCACT 
GAGGGAAAGG CTTTAATTTT GCCTAATAGA GAGGGAATGC CACAGCCAGA AGTGTCTCCA 
CTTGTAGAAG AGGAATCCTC CGTGCCCCTT GTTGCCTTTA ACCAAGTAGA GATCCAGTCT 
AATGAAGATC CTGAAGATTT GGAGACTGAG ATACTTGAAG AAACAGAAGG CAAGGAGAAA 
GAGGGCAATG AGTGCCCAGT CCACGTTACA AATGTTTGGA CTGAGCAGAC CAAACAAGAA 
AGTGAGGGAC AGGACGCTGT TGAATGCGAG CAACCAAATG AAGATGCCAA ACTGGATACT 
GAATGCTCAG AACAAGAGCA AAATATAGAG AATCAGTCAA ATAGAGAGTC AAGTGTACCC 
AAAGCTTTAT TTGAAAAGGT GCCACTCCCT CAAAGTGTAG TCACTCCAAC ATTCTCTGCC 
CAGTCATCCT TCGATGATTC CTTACCGCCT CGTTCTCGAT CAGCTTCTCC AACCAAAACC 
AAAGGCAAAT CAGCACTGCT GATTGGACTC TCTGCTGGTT TATTTGATGC AAACAACCCA 
AAGATGCTGC GCACATGTTC GTTGCCCGAC CTTAGCAAAT TATTCAAGAC CTTTGTGGAT 
GAGCCCACAG TGGCTGATGT AGAAGGGGAG GAGCAGTTGG AAATAGAAGA TATAGAGGAT 
GAACCTGTTC AGGATGCGGA TCACTCTGAT TCAGAGGACA CCATGTTTGG GGAGACAGAC 
CGAGATTTAC AGGAAATGCA GGCATCAATG GAACAACTAT TGAGAGAGGA GCCTACGGAA 
GATTACTGCG CAGAGAAAGC AACCCATTTA AACTCAGAAC CACCAGCAGG AATCAAAGAG 
GAAAATATTG AGTCTGCCAT TATTAATGGG GCAGAGGAGG AGGAGGAGGA AGATGAAGAA 
GAAGAAGATG GAAATCAGCC AAGTGGTGAA AGTGTCATTA ATGAAGAATG GCATTCGGAT 
GATAGCAGTG AGGACTTTGC CAGTGAATGT GATGATGGTG ATAGCCTTTT TGCCCATCTG 
GAAGAAATGA GGTTTAAACT GGAACAAGAG ATTGGTTTTG AAAAATTCAT AGAAGTCTAC 
AATAAAATCA AGGCTATACA CGAAGATGAG GATGAAAATA TTGATAGTTG TTCAACAGTC 
GTGGAGAATA TTCTAGGCTG TGAGCACCAG CATTTGTATC CCAGAGTCCT TCGCCTAGTG 
ATGGCAGATG GAGCTTATCA AGAAGATAAT GACGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nek1 ( XM_012971595.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012827049.1 Xenopus tropicalis NIMA-related kinase 1 (nek1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa29842
Clone ID Related Accession (Same CDS sequence)	XM_012971595.2
Accession Version	XM_012971595.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3963bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	serine/threonine-protein kinase Nek1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012971595.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATAAGT ACGTGAGAGT CAGCAAGATC GGAGAAGGTT CCTTTGGAAA GGCCATTCTG 
GTGAAATCTA AAGATGATGG CAAGCAATAT GTCATCAAAG AAATCGGCAT CTCCAAGATG 
TCAAACAAGG AGAGGGAGGA ATCCAGGAGG GAAGTTGCTG TGTTGGCAAA TATGAAGCAT 
CCAAATATTG TCCAATACCA AGAGTCATTT GAAGAAAGTG GATGTCTTTA CATTGTGATG 
GATTATTGTG AAGGAGGGGA CCTATTTAAA AGAATAAATT CACAGAAAGG GGTTTTGTTT 
TCAGAAGATC AGATCCTGGA CTGGTTTGTG CAGCTGTGCC TGGCTCTAAA GCATGTACAT 
GACAGGAAGA TTCTGCATCG GGACATAAAG TCCCAGAATA TCTTTCTAAC AAAAGGTGGT 
ACAATACAAC TGGGTGATTT TGGAATAGCA CGGGTGTTAA ACAGCACTGT AGAGCTGGCT 
CGCACCTGTA TAGGAACTCC GTACTATCTG TCTCCTGAGA TATGTGAAAA CAGGCCATAT 
AACAACAAGA GTGATATTTG GGCGCTGGGC TGTGTTTTGT ATGAAATGTG CACCCTCAAG 
CATGCGTTTG AAGCTGGCAA CATGAAGAAC TTAGTACTGA AAATAATCCG AGGCTCCTAT 
CCCCCTGTGT CTGTGCATTA TTCTTATGAC CTACGCAATC TGCTCTCAAT GCTTTTTAAG 
AGGAATCCTA GGGATCGTCC ATCTGTCAAC TCCATATTGG AGAAGCCTTT TATATATAGA 
CGTATTGAGA AGTTTTTGTC CCCACAGCAT ATTGCTGAAG AGTTTGGCCT GGGAGCCTTT 
GTGAATCACC CCAAACCTGG GATCCAAGCT GCAGCAGCCA AAAGACCAAC TCCAGCACAA 
GTGGTTCCTG CTTCCCCTCC TGCTCACAAA ATCACTAAAC CTGCTGCTAA ATACGGAGTG 
CCTTTAACAA CAAAAAAATC TTCAGATGCT GCAAAGAAGC ATCATGATAA GAAACCCCAA 
ATCAGACACA GACAGGCCCC AGCAGTACCA CTGAAGAAAG TGAATCCTGT AGACGAAAGG 
AGGAAAATGT TTGAGGACGG GGCCAAGAAA AAAAGGATGG ATGTTAGACA ACTGGAGAAG 
GAAAAAAGAC AACGGGAACA GTTTAATCTA CTAAGGGCCG AACAGATGAG GAGACTGGAA 
AAGGAAAGGT TGGAGCGAAT AAATCGTGCA CGAGAGCAAG GCTGGAGAAA TGTAATCAGC 
TCAGGAGGAA GCGGAGATAT TAAGGCACCT TATTTTGGTG GAGGGGGAGG TGTAGCACCC 
CCACAAGGCA TGGGTAGAGG TCCCTATGAG CATTACCATT CCATTTTTGA TCAGATGCAG 
CAGCAGAAGG AAAACATCAA AGCAGCAGTG GAAAAAGAGG TGAAACTAAA GGCAGATATC 
CAAAATCCTG ACACTTATAG CAGAGTTCCT AAGCATCCTG AACAAGAAAA TTGTCCTTCA 
GCTCATTCTC CATTCCCAGA TGGTGATGCA GTGAGAAGGG AACTGAAGAG GCTGCAAGAA 
GTACACAATC AAGCCATTGG CAACAAGATT CAAGGACACG TTTCTGCAGA AAAGGCAAGA 
CAGGTTGAAG AGTTTTTGCA ACGGAAGCAT GAAGCAATGC AAAATAAGGC TCGAGCAGAG 
GGACATATGG ACGCAGTGCA CAACCTGGCA GCTATCTGTG GAGGCAGGCC AAATTCTGCA 
GGAGGAGGCA AGGCACGCAA CAGAGAAGAA AAGGAGTATT TAGCAAGACT TAGACAAATC 
AGACTCCAGA ATTTTAATGA GCGACAACAA ATAAAAGCCA AGCTGAGAGG GGCAAAGGGG 
GATGAAGGTG ACGCAGATGG TAAGGAAAGC AGTGAAGACG CTGAACTAAG AAGAAAGAAA 
ATTGAAGCTT TGAAGGCTCA AGCAAATGCA CGAGCTGCTT TAATAAAAGA AGAACTTGAG 
AGAAAGAGAA GAGAAGCAGA TAAAAGAGAG AAGAGACTAT GGGAAGAGCA TCTGGCGTTA 
AAAAATCCTA ATGTGCCTTG TAATGCAGTT GGGGCAGCAC CTGGTTCCCC CAAACATTCA 
CATCTTGACG TTGGGGGATC ACCTGTCCGA GGGCATCCTC ATGCGAAACC CAGCACACCT 
GCCATATCTA TGACTTCAGC GCTAAGGAAT GTTGGGGCTG AAGGCGAAGA TACAGGTATC 
AGGGATCCAA CAGAGGGCTC CAAAACATCA GACACCTCAG TACAAAATGA AAAGCGGCAA 
ATACTCCGCC GATTAAATAA TAACATGAAA TCTCAGGAAG AAACAGAAGC CACAAACCCG 
CCAACAGAGG AAAATGGAGT GCAAACCAAT GGCGAGGAAC AGCAAGAGAG TGGTGAGCCT 
CTGGCAGTAG ACAGAAAGAA GTGGCAGGCC GAGCCTCCTC CTGTTATCCC TCTTTCCCAG 
CTCACACTTG TAGAGGATAC AGTGTTCCCA TTGGACAATC TAACAGTTGG AGATGTCATC 
AAACTGGATG TGGAAGAACC CCAGAGACGA CTGTGGGGGA AAGATCCTGG TGATTCTGTG 
TTAAAGGTAC TCGGTGAGGC CAAACTCCAG CTTCAAACAG AATTGCTTCA AGTAACAATC 
GATGAACAGT GCCATGAAGA AGCAGATGAA GAGAAGGAAA ACCCTGACAT GCGGGACAAT 
TTGTTAAATA AAGTTTCACC CACTGAGGGA AAGGCTTTAA TTTTGCCTAA TAGAGAGGGA 
ATGCCACAGC CAGAAGTGTC TCCACTTGTA GAAGAGGAAT CCTCCGTGCC CCTTGTTGCC 
TTTAACCAAG TAGAGATCCA GTCTAATGAA GATCCTGAAG ATTTGGAGAC TGAGATACTT 
GAAGAAACAG AAGGCAAGGA GAAAGAGGGC AATGAGTGCC CAGTCCACGT TACAAATGTT 
TGGACTGAGC AGACCAAACA AGAAAGTGAG GGACAGGACC CTGTTGAATG CGAGCAACCA 
AATGAAGATG CCAAACTGGA TACTGAATGC TCAGAACAAG AGCAAAATAT AGAGAATCAG 
TCAAATAGAG AGTCAAGTGT ACCCAAAGCT TTATTTGAAA AGGTGCCACT CCCTCAAAGT 
GTAGTCACTC CAACATTCTC TGCCCAGTCA TCCTTCGATG ATTCCTTACC GCCTCGTTCT 
CGATCAGCTT CTCCAACCAA AACCAAAGGC AAATCAGCAC TGCTGATTGG ACTCTCTGCT 
GGTTTATTTG ATGCAAACAA CCCAAAGATG CTGCGCACAT GTTCGTTGCC CGACCTTAGC 
AAATTATTCA AGACCTTTGT GGATGAGCCC ACAGTGGCTG ATGTAGAAGG GGAGGAGCAG 
TTGGAAATAG AAGATATAGA GGATGAACCT GTTCAGGATG CGGATCACTC TGATTCAGAG 
GACACCATGT TTGGGGAGAC AGACCGAGAT TTACAGGAAA TGCAGGCATC AATGGAACAA 
CTATTGAGAG AGGAGCCTAC GGAAGATTAC TGCGCAGAGA AAGCAACCCA TTTAAACTCA 
GAACCACCAG CAGGAATCAA AGAGGAAAAT ATTGAGTCTG CCATTATTAA TGGGGCAGAG 
GAGGAGGAGG AGGAAGATGA AGAAGAAGAA GATGGAAATC AGCCAAGTGG TGAAAGTGTC 
ATTAATGAAG AATGGCATTC GGATGATAGC AGTGAGGACT TTGCCAGTGA ATGTGATGAT 
GGTGATAGCC TTTTTGCCCA TCTGGAAGAA ATGAGGTTTA AACTGGAACA AGAGATTGGT 
TTTGAAAAAT TCATAGAAGT CTACAATAAA ATCAAGGCTA TACACGAAGA TGAGGATGAA 
AATATTGATA GTTGTTCAAC AGTCGTGGAG AATATTCTAG GCTGTGAGCA CCAGCATTTG 
TATCCCAGAG TCCTTCGCCT AGTGATGGCA GATGGAGCTT ATCAAGAAGA TAATGACGAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012827049.1
CDS	178..4140
Translation	MNKYVRVSKIGEGSFGKAILVKSKDDGKQYVIKEIGISKMSNKEREESRREVAVLANMKHPNIVQYQESFEESGCLYIVMDYCEGGDLFKRINSQKGVLFSEDQILDWFVQLCLALKHVHDRKILHRDIKSQNIFLTKGGTIQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENRPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIIRGSYPPVSVHYSYDLRNLLSMLFKRNPRDRPSVNSILEKPFIYRRIEKFLSPQHIAEEFGLGAFVNHPKPGIQAAAAKRPTPAQVVPASPPAHKITKPAAKYGVPLTTKKSSDAAKKHHDKKPQIRHRQAPAVPLKKVNPVDERRKMFEDGAKKKRMDVRQLEKEKRQREQFNLLRAEQMRRLEKERLERINRAREQGWRNVISSGGSGDIKAPYFGGGGGVAPPQGMGRGPYEHYHSIFDQMQQQKENIKAAVEKEVKLKADIQNPDTYSRVPKHPEQENCPSAHSPFPDGDAVRRELKRLQEVHNQAIGNKIQGHVSAEKARQVEEFLQRKHEAMQNKARAEGHMDAVHNLAAICGGRPNSAGGGKARNREEKEYLARLRQIRLQNFNERQQIKAKLRGAKGDEGDADGKESSEDAELRRKKIEALKAQANARAALIKEELERKRREADKREKRLWEEHLALKNPNVPCNAVGAAPGSPKHSHLDVGGSPVRGHPHAKPSTPAISMTSALRNVGAEGEDTGIRDPTEGSKTSDTSVQNEKRQILRRLNNNMKSQEETEATNPPTEENGVQTNGEEQQESGEPLAVDRKKWQAEPPPVIPLSQLTLVEDTVFPLDNLTVGDVIKLDVEEPQRRLWGKDPGDSVLKVLGEAKLQLQTELLQVTIDEQCHEEADEEKENPDMRDNLLNKVSPTEGKALILPNREGMPQPEVSPLVEEESSVPLVAFNQVEIQSNEDPEDLETEILEETEGKEKEGNECPVHVTNVWTEQTKQESEGQDPVECEQPNEDAKLDTECSEQEQNIENQSNRESSVPKALFEKVPLPQSVVTPTFSAQSSFDDSLPPRSRSASPTKTKGKSALLIGLSAGLFDANNPKMLRTCSLPDLSKLFKTFVDEPTVADVEGEEQLEIEDIEDEPVQDADHSDSEDTMFGETDRDLQEMQASMEQLLREEPTEDYCAEKATHLNSEPPAGIKEENIESAIINGAEEEEEEDEEEEDGNQPSGESVINEEWHSDDSSEDFASECDDGDSLFAHLEEMRFKLEQEIGFEKFIEVYNKIKAIHEDEDENIDSCSTVVENILGCEHQHLYPRVLRLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_012971595.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis NIMA-related kinase 1 (nek1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012971595.2

ORF Insert Sequence:

ATGAATAAGT ACGTGAGAGT CAGCAAGATC GGAGAAGGTT CCTTTGGAAA GGCCATTCTG 
GTGAAATCTA AAGATGATGG CAAGCAATAT GTCATCAAAG AAATCGGCAT CTCCAAGATG 
TCAAACAAGG AGAGGGAGGA ATCCAGGAGG GAAGTTGCTG TGTTGGCAAA TATGAAGCAT 
CCAAATATTG TCCAATACCA AGAGTCATTT GAAGAAAGTG GATGTCTTTA CATTGTGATG 
GATTATTGTG AAGGAGGGGA CCTATTTAAA AGAATAAATT CACAGAAAGG GGTTTTGTTT 
TCAGAAGATC AGATCCTGGA CTGGTTTGTG CAGCTGTGCC TGGCTCTAAA GCATGTACAT 
GACAGGAAGA TTCTGCATCG GGACATAAAG TCCCAGAATA TCTTTCTAAC AAAAGGTGGT 
ACAATACAAC TGGGTGATTT TGGAATAGCA CGGGTGTTAA ACAGCACTGT AGAGCTGGCT 
CGCACCTGTA TAGGAACTCC GTACTATCTG TCTCCTGAGA TATGTGAAAA CAGGCCATAT 
AACAACAAGA GTGATATTTG GGCGCTGGGC TGTGTTTTGT ATGAAATGTG CACCCTCAAG 
CATGCGTTTG AAGCTGGCAA CATGAAGAAC TTAGTACTGA AAATAATCCG AGGCTCCTAT 
CCCCCTGTGT CTGTGCATTA TTCTTATGAC CTACGCAATC TGCTCTCAAT GCTTTTTAAG 
AGGAATCCTA GGGATCGTCC ATCTGTCAAC TCCATATTGG AGAAGCCTTT TATATATAGA 
CGTATTGAGA AGTTTTTGTC CCCACAGCAT ATTGCTGAAG AGTTTGGCCT GGGAGCCTTT 
GTGAATCACC CCAAACCTGG GATCCAAGCT GCAGCAGCCA AAAGACCAAC TCCAGCACAA 
GTGGTTCCTG CTTCCCCTCC TGCTCACAAA ATCACTAAAC CTGCTGCTAA ATACGGAGTG 
CCTTTAACAA CAAAAAAATC TTCAGATGCT GCAAAGAAGC ATCATGATAA GAAACCCCAA 
ATCAGACACA GACAGGCCCC AGCAGTACCA CTGAAGAAAG TGAATCCTGT AGACGAAAGG 
AGGAAAATGT TTGAGGACGG GGCCAAGAAA AAAAGGATGG ATGTTAGACA ACTGGAGAAG 
GAAAAAAGAC AACGGGAACA GTTTAATCTA CTAAGGGCCG AACAGATGAG GAGACTGGAA 
AAGGAAAGGT TGGAGCGAAT AAATCGTGCA CGAGAGCAAG GCTGGAGAAA TGTAATCAGC 
TCAGGAGGAA GCGGAGATAT TAAGGCACCT TATTTTGGTG GAGGGGGAGG TGTAGCACCC 
CCACAAGGCA TGGGTAGAGG TCCCTATGAG CATTACCATT CCATTTTTGA TCAGATGCAG 
CAGCAGAAGG AAAACATCAA AGCAGCAGTG GAAAAAGAGG TGAAACTAAA GGCAGATATC 
CAAAATCCTG ACACTTATAG CAGAGTTCCT AAGCATCCTG AACAAGAAAA TTGTCCTTCA 
GCTCATTCTC CATTCCCAGA TGGTGATGCA GTGAGAAGGG AACTGAAGAG GCTGCAAGAA 
GTACACAATC AAGCCATTGG CAACAAGATT CAAGGACACG TTTCTGCAGA AAAGGCAAGA 
CAGGTTGAAG AGTTTTTGCA ACGGAAGCAT GAAGCAATGC AAAATAAGGC TCGAGCAGAG 
GGACATATGG ACGCAGTGCA CAACCTGGCA GCTATCTGTG GAGGCAGGCC AAATTCTGCA 
GGAGGAGGCA AGGCACGCAA CAGAGAAGAA AAGGAGTATT TAGCAAGACT TAGACAAATC 
AGACTCCAGA ATTTTAATGA GCGACAACAA ATAAAAGCCA AGCTGAGAGG GGCAAAGGGG 
GATGAAGGTG ACGCAGATGG TAAGGAAAGC AGTGAAGACG CTGAACTAAG AAGAAAGAAA 
ATTGAAGCTT TGAAGGCTCA AGCAAATGCA CGAGCTGCTT TAATAAAAGA AGAACTTGAG 
AGAAAGAGAA GAGAAGCAGA TAAAAGAGAG AAGAGACTAT GGGAAGAGCA TCTGGCGTTA 
AAAAATCCTA ATGTGCCTTG TAATGCAGTT GGGGCAGCAC CTGGTTCCCC CAAACATTCA 
CATCTTGACG TTGGGGGATC ACCTGTCCGA GGGCATCCTC ATGCGAAACC CAGCACACCT 
GCCATATCTA TGACTTCAGC GCTAAGGAAT GTTGGGGCTG AAGGCGAAGA TACAGGTATC 
AGGGATCCAA CAGAGGGCTC CAAAACATCA GACACCTCAG TACAAAATGA AAAGCGGCAA 
ATACTCCGCC GATTAAATAA TAACATGAAA TCTCAGGAAG AAACAGAAGC CACAAACCCG 
CCAACAGAGG AAAATGGAGT GCAAACCAAT GGCGAGGAAC AGCAAGAGAG TGGTGAGCCT 
CTGGCAGTAG ACAGAAAGAA GTGGCAGGCC GAGCCTCCTC CTGTTATCCC TCTTTCCCAG 
CTCACACTTG TAGAGGATAC AGTGTTCCCA TTGGACAATC TAACAGTTGG AGATGTCATC 
AAACTGGATG TGGAAGAACC CCAGAGACGA CTGTGGGGGA AAGATCCTGG TGATTCTGTG 
TTAAAGGTAC TCGGTGAGGC CAAACTCCAG CTTCAAACAG AATTGCTTCA AGTAACAATC 
GATGAACAGT GCCATGAAGA AGCAGATGAA GAGAAGGAAA ACCCTGACAT GCGGGACAAT 
TTGTTAAATA AAGTTTCACC CACTGAGGGA AAGGCTTTAA TTTTGCCTAA TAGAGAGGGA 
ATGCCACAGC CAGAAGTGTC TCCACTTGTA GAAGAGGAAT CCTCCGTGCC CCTTGTTGCC 
TTTAACCAAG TAGAGATCCA GTCTAATGAA GATCCTGAAG ATTTGGAGAC TGAGATACTT 
GAAGAAACAG AAGGCAAGGA GAAAGAGGGC AATGAGTGCC CAGTCCACGT TACAAATGTT 
TGGACTGAGC AGACCAAACA AGAAAGTGAG GGACAGGACC CTGTTGAATG CGAGCAACCA 
AATGAAGATG CCAAACTGGA TACTGAATGC TCAGAACAAG AGCAAAATAT AGAGAATCAG 
TCAAATAGAG AGTCAAGTGT ACCCAAAGCT TTATTTGAAA AGGTGCCACT CCCTCAAAGT 
GTAGTCACTC CAACATTCTC TGCCCAGTCA TCCTTCGATG ATTCCTTACC GCCTCGTTCT 
CGATCAGCTT CTCCAACCAA AACCAAAGGC AAATCAGCAC TGCTGATTGG ACTCTCTGCT 
GGTTTATTTG ATGCAAACAA CCCAAAGATG CTGCGCACAT GTTCGTTGCC CGACCTTAGC 
AAATTATTCA AGACCTTTGT GGATGAGCCC ACAGTGGCTG ATGTAGAAGG GGAGGAGCAG 
TTGGAAATAG AAGATATAGA GGATGAACCT GTTCAGGATG CGGATCACTC TGATTCAGAG 
GACACCATGT TTGGGGAGAC AGACCGAGAT TTACAGGAAA TGCAGGCATC AATGGAACAA 
CTATTGAGAG AGGAGCCTAC GGAAGATTAC TGCGCAGAGA AAGCAACCCA TTTAAACTCA 
GAACCACCAG CAGGAATCAA AGAGGAAAAT ATTGAGTCTG CCATTATTAA TGGGGCAGAG 
GAGGAGGAGG AGGAAGATGA AGAAGAAGAA GATGGAAATC AGCCAAGTGG TGAAAGTGTC 
ATTAATGAAG AATGGCATTC GGATGATAGC AGTGAGGACT TTGCCAGTGA ATGTGATGAT 
GGTGATAGCC TTTTTGCCCA TCTGGAAGAA ATGAGGTTTA AACTGGAACA AGAGATTGGT 
TTTGAAAAAT TCATAGAAGT CTACAATAAA ATCAAGGCTA TACACGAAGA TGAGGATGAA 
AATATTGATA GTTGTTCAAC AGTCGTGGAG AATATTCTAG GCTGTGAGCA CCAGCATTTG 
TATCCCAGAG TCCTTCGCCT AGTGATGGCA GATGGAGCTT ATCAAGAAGA TAATGACGAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nek1 ( XM_012952724.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012808178.1 Xenopus tropicalis NIMA-related kinase 1 (nek1), transcript variant X2, mRNA.

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CloneID	OXa29843
Clone ID Related Accession (Same CDS sequence)	XM_012952724.2
Accession Version	XM_012952724.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3903bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	serine/threonine-protein kinase Nek1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012952724.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATAAGT ACGTGAGAGT CAGCAAGATC GGAGAAGGTT CCTTTGGAAA GGCCATTCTG 
GTGAAATCTA AAGATGATGG CAAGCAATAT GTCATCAAAG AAATCGGCAT CTCCAAGATG 
TCAAACAAGG AGAGGGAGGA ATCCAGGAGG GAAGTTGCTG TGTTGGCAAA TATGAAGCAT 
CCAAATATTG TCCAATACCA AGAGTCATTT GAAGAAAGTG GATGTCTTTA CATTGTGATG 
GATTATTGTG AAGGAGGGGA CCTATTTAAA AGAATAAATT CACAGAAAGG GGTTTTGTTT 
TCAGAAGATC AGATCCTGGA CTGGTTTGTG CAGCTGTGCC TGGCTCTAAA GCATGTACAT 
GACAGGAAGA TTCTGCATCG GGACATAAAG TCCCAGAATA TCTTTCTAAC AAAAGGTGGT 
ACAATACAAC TGGGTGATTT TGGAATAGCA CGGGTGTTAA ACAGCACTGT AGAGCTGGCT 
CGCACCTGTA TAGGAACTCC GTACTATCTG TCTCCTGAGA TATGTGAAAA CAGGCCATAT 
AACAACAAGA GTGATATTTG GGCGCTGGGC TGTGTTTTGT ATGAAATGTG CACCCTCAAG 
CATGCGTTTG AAGCTGGCAA CATGAAGAAC TTAGTACTGA AAATAATCCG AGGCTCCTAT 
CCCCCTGTGT CTGTGCATTA TTCTTATGAC CTACGCAATC TGCTCTCAAT GCTTTTTAAG 
AGGAATCCTA GGGATCGTCC ATCTGTCAAC TCCATATTGG AGAAGCCTTT TATATATAGA 
CGTATTGAGA AGTTTTTGTC CCCACAGCAT ATTGCTGAAG AGTTTGGCCT GGGAGCCTTT 
GTGAATCACC CCAAACCTGG GATCCAAGCT GCAGCAGCCA AAAGACCAAC TCCAGCACAA 
GTGGTTCCTG CTTCCCCTCC TGCTCACAAA ATCACTAAAC CTGCTGCTAA ATACGGAGTG 
CCTTTAACAA CAAAAAAATC TTCAGATGCT GCAAAGAAGC ATCATGATAA GAAACCCCAA 
ATCAGACACA GACAGGACGG GGCCAAGAAA AAAAGGATGG ATGTTAGACA ACTGGAGAAG 
GAAAAAAGAC AACGGGAACA GTTTAATCTA CTAAGGGCCG AACAGATGAG GAGACTGGAA 
AAGGAAAGGT TGGAGCGAAT AAATCGTGCA CGAGAGCAAG GCTGGAGAAA TGTAATCAGC 
TCAGGAGGAA GCGGAGATAT TAAGGCACCT TATTTTGGTG GAGGGGGAGG TGTAGCACCC 
CCACAAGGCA TGGGTAGAGG TCCCTATGAG CATTACCATT CCATTTTTGA TCAGATGCAG 
CAGCAGAAGG AAAACATCAA AGCAGCAGTG GAAAAAGAGG TGAAACTAAA GGCAGATATC 
CAAAATCCTG ACACTTATAG CAGAGTTCCT AAGCATCCTG AACAAGAAAA TTGTCCTTCA 
GCTCATTCTC CATTCCCAGA TGGTGATGCA GTGAGAAGGG AACTGAAGAG GCTGCAAGAA 
GTACACAATC AAGCCATTGG CAACAAGATT CAAGGACACG TTTCTGCAGA AAAGGCAAGA 
CAGGTTGAAG AGTTTTTGCA ACGGAAGCAT GAAGCAATGC AAAATAAGGC TCGAGCAGAG 
GGACATATGG ACGCAGTGCA CAACCTGGCA GCTATCTGTG GAGGCAGGCC AAATTCTGCA 
GGAGGAGGCA AGGCACGCAA CAGAGAAGAA AAGGAGTATT TAGCAAGACT TAGACAAATC 
AGACTCCAGA ATTTTAATGA GCGACAACAA ATAAAAGCCA AGCTGAGAGG GGCAAAGGGG 
GATGAAGGTG ACGCAGATGG TAAGGAAAGC AGTGAAGACG CTGAACTAAG AAGAAAGAAA 
ATTGAAGCTT TGAAGGCTCA AGCAAATGCA CGAGCTGCTT TAATAAAAGA AGAACTTGAG 
AGAAAGAGAA GAGAAGCAGA TAAAAGAGAG AAGAGACTAT GGGAAGAGCA TCTGGCGTTA 
AAAAATCCTA ATGTGCCTTG TAATGCAGTT GGGGCAGCAC CTGGTTCCCC CAAACATTCA 
CATCTTGACG TTGGGGGATC ACCTGTCCGA GGGCATCCTC ATGCGAAACC CAGCACACCT 
GCCATATCTA TGACTTCAGC GCTAAGGAAT GTTGGGGCTG AAGGCGAAGA TACAGGTATC 
AGGGATCCAA CAGAGGGCTC CAAAACATCA GACACCTCAG TACAAAATGA AAAGCGGCAA 
ATACTCCGCC GATTAAATAA TAACATGAAA TCTCAGGAAG AAACAGAAGC CACAAACCCG 
CCAACAGAGG AAAATGGAGT GCAAACCAAT GGCGAGGAAC AGCAAGAGAG TGGTGAGCCT 
CTGGCAGTAG ACAGAAAGAA GTGGCAGGCC GAGCCTCCTC CTGTTATCCC TCTTTCCCAG 
CTCACACTTG TAGAGGATAC AGTGTTCCCA TTGGACAATC TAACAGTTGG AGATGTCATC 
AAACTGGATG TGGAAGAACC CCAGAGACGA CTGTGGGGGA AAGATCCTGG TGATTCTGTG 
TTAAAGGTAC TCGGTGAGGC CAAACTCCAG CTTCAAACAG AATTGCTTCA AGTAACAATC 
GATGAACAGT GCCATGAAGA AGCAGATGAA GAGAAGGAAA ACCCTGACAT GCGGGACAAT 
TTGTTAAATA AAGTTTCACC CACTGAGGGA AAGGCTTTAA TTTTGCCTAA TAGAGAGGGA 
ATGCCACAGC CAGAAGTGTC TCCACTTGTA GAAGAGGAAT CCTCCGTGCC CCTTGTTGCC 
TTTAACCAAG TAGAGATCCA GTCTAATGAA GATCCTGAAG ATTTGGAGAC TGAGATACTT 
GAAGAAACAG AAGGCAAGGA GAAAGAGGGC AATGAGTGCC CAGTCCACGT TACAAATGTT 
TGGACTGAGC AGACCAAACA AGAAAGTGAG GGACAGGACC CTGTTGAATG CGAGCAACCA 
AATGAAGATG CCAAACTGGA TACTGAATGC TCAGAACAAG AGCAAAATAT AGAGAATCAG 
TCAAATAGAG AGTCAAGTGT ACCCAAAGCT TTATTTGAAA AGGTGCCACT CCCTCAAAGT 
GTAGTCACTC CAACATTCTC TGCCCAGTCA TCCTTCGATG ATTCCTTACC GCCTCGTTCT 
CGATCAGCTT CTCCAACCAA AACCAAAGGC AAATCAGCAC TGCTGATTGG ACTCTCTGCT 
GGTTTATTTG ATGCAAACAA CCCAAAGATG CTGCGCACAT GTTCGTTGCC CGACCTTAGC 
AAATTATTCA AGACCTTTGT GGATGAGCCC ACAGTGGCTG ATGTAGAAGG GGAGGAGCAG 
TTGGAAATAG AAGATATAGA GGATGAACCT GTTCAGGATG CGGATCACTC TGATTCAGAG 
GACACCATGT TTGGGGAGAC AGACCGAGAT TTACAGGAAA TGCAGGCATC AATGGAACAA 
CTATTGAGAG AGGAGCCTAC GGAAGATTAC TGCGCAGAGA AAGCAACCCA TTTAAACTCA 
GAACCACCAG CAGGAATCAA AGAGGAAAAT ATTGAGTCTG CCATTATTAA TGGGGCAGAG 
GAGGAGGAGG AGGAAGATGA AGAAGAAGAA GATGGAAATC AGCCAAGTGG TGAAAGTGTC 
ATTAATGAAG AATGGCATTC GGATGATAGC AGTGAGGACT TTGCCAGTGA ATGTGATGAT 
GGTGATAGCC TTTTTGCCCA TCTGGAAGAA ATGAGGTTTA AACTGGAACA AGAGATTGGT 
TTTGAAAAAT TCATAGAAGT CTACAATAAA ATCAAGGCTA TACACGAAGA TGAGGATGAA 
AATATTGATA GTTGTTCAAC AGTCGTGGAG AATATTCTAG GCTGTGAGCA CCAGCATTTG 
TATCCCAGAG TCCTTCGCCT AGTGATGGCA GATGGAGCTT ATCAAGAAGA TAATGACGAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012808178.1
CDS	178..4080
Translation	MNKYVRVSKIGEGSFGKAILVKSKDDGKQYVIKEIGISKMSNKEREESRREVAVLANMKHPNIVQYQESFEESGCLYIVMDYCEGGDLFKRINSQKGVLFSEDQILDWFVQLCLALKHVHDRKILHRDIKSQNIFLTKGGTIQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENRPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIIRGSYPPVSVHYSYDLRNLLSMLFKRNPRDRPSVNSILEKPFIYRRIEKFLSPQHIAEEFGLGAFVNHPKPGIQAAAAKRPTPAQVVPASPPAHKITKPAAKYGVPLTTKKSSDAAKKHHDKKPQIRHRQDGAKKKRMDVRQLEKEKRQREQFNLLRAEQMRRLEKERLERINRAREQGWRNVISSGGSGDIKAPYFGGGGGVAPPQGMGRGPYEHYHSIFDQMQQQKENIKAAVEKEVKLKADIQNPDTYSRVPKHPEQENCPSAHSPFPDGDAVRRELKRLQEVHNQAIGNKIQGHVSAEKARQVEEFLQRKHEAMQNKARAEGHMDAVHNLAAICGGRPNSAGGGKARNREEKEYLARLRQIRLQNFNERQQIKAKLRGAKGDEGDADGKESSEDAELRRKKIEALKAQANARAALIKEELERKRREADKREKRLWEEHLALKNPNVPCNAVGAAPGSPKHSHLDVGGSPVRGHPHAKPSTPAISMTSALRNVGAEGEDTGIRDPTEGSKTSDTSVQNEKRQILRRLNNNMKSQEETEATNPPTEENGVQTNGEEQQESGEPLAVDRKKWQAEPPPVIPLSQLTLVEDTVFPLDNLTVGDVIKLDVEEPQRRLWGKDPGDSVLKVLGEAKLQLQTELLQVTIDEQCHEEADEEKENPDMRDNLLNKVSPTEGKALILPNREGMPQPEVSPLVEEESSVPLVAFNQVEIQSNEDPEDLETEILEETEGKEKEGNECPVHVTNVWTEQTKQESEGQDPVECEQPNEDAKLDTECSEQEQNIENQSNRESSVPKALFEKVPLPQSVVTPTFSAQSSFDDSLPPRSRSASPTKTKGKSALLIGLSAGLFDANNPKMLRTCSLPDLSKLFKTFVDEPTVADVEGEEQLEIEDIEDEPVQDADHSDSEDTMFGETDRDLQEMQASMEQLLREEPTEDYCAEKATHLNSEPPAGIKEENIESAIINGAEEEEEEDEEEEDGNQPSGESVINEEWHSDDSSEDFASECDDGDSLFAHLEEMRFKLEQEIGFEKFIEVYNKIKAIHEDEDENIDSCSTVVENILGCEHQHLYPRVLRLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_012952724.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis NIMA-related kinase 1 (nek1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012952724.2

ORF Insert Sequence:

ATGAATAAGT ACGTGAGAGT CAGCAAGATC GGAGAAGGTT CCTTTGGAAA GGCCATTCTG 
GTGAAATCTA AAGATGATGG CAAGCAATAT GTCATCAAAG AAATCGGCAT CTCCAAGATG 
TCAAACAAGG AGAGGGAGGA ATCCAGGAGG GAAGTTGCTG TGTTGGCAAA TATGAAGCAT 
CCAAATATTG TCCAATACCA AGAGTCATTT GAAGAAAGTG GATGTCTTTA CATTGTGATG 
GATTATTGTG AAGGAGGGGA CCTATTTAAA AGAATAAATT CACAGAAAGG GGTTTTGTTT 
TCAGAAGATC AGATCCTGGA CTGGTTTGTG CAGCTGTGCC TGGCTCTAAA GCATGTACAT 
GACAGGAAGA TTCTGCATCG GGACATAAAG TCCCAGAATA TCTTTCTAAC AAAAGGTGGT 
ACAATACAAC TGGGTGATTT TGGAATAGCA CGGGTGTTAA ACAGCACTGT AGAGCTGGCT 
CGCACCTGTA TAGGAACTCC GTACTATCTG TCTCCTGAGA TATGTGAAAA CAGGCCATAT 
AACAACAAGA GTGATATTTG GGCGCTGGGC TGTGTTTTGT ATGAAATGTG CACCCTCAAG 
CATGCGTTTG AAGCTGGCAA CATGAAGAAC TTAGTACTGA AAATAATCCG AGGCTCCTAT 
CCCCCTGTGT CTGTGCATTA TTCTTATGAC CTACGCAATC TGCTCTCAAT GCTTTTTAAG 
AGGAATCCTA GGGATCGTCC ATCTGTCAAC TCCATATTGG AGAAGCCTTT TATATATAGA 
CGTATTGAGA AGTTTTTGTC CCCACAGCAT ATTGCTGAAG AGTTTGGCCT GGGAGCCTTT 
GTGAATCACC CCAAACCTGG GATCCAAGCT GCAGCAGCCA AAAGACCAAC TCCAGCACAA 
GTGGTTCCTG CTTCCCCTCC TGCTCACAAA ATCACTAAAC CTGCTGCTAA ATACGGAGTG 
CCTTTAACAA CAAAAAAATC TTCAGATGCT GCAAAGAAGC ATCATGATAA GAAACCCCAA 
ATCAGACACA GACAGGACGG GGCCAAGAAA AAAAGGATGG ATGTTAGACA ACTGGAGAAG 
GAAAAAAGAC AACGGGAACA GTTTAATCTA CTAAGGGCCG AACAGATGAG GAGACTGGAA 
AAGGAAAGGT TGGAGCGAAT AAATCGTGCA CGAGAGCAAG GCTGGAGAAA TGTAATCAGC 
TCAGGAGGAA GCGGAGATAT TAAGGCACCT TATTTTGGTG GAGGGGGAGG TGTAGCACCC 
CCACAAGGCA TGGGTAGAGG TCCCTATGAG CATTACCATT CCATTTTTGA TCAGATGCAG 
CAGCAGAAGG AAAACATCAA AGCAGCAGTG GAAAAAGAGG TGAAACTAAA GGCAGATATC 
CAAAATCCTG ACACTTATAG CAGAGTTCCT AAGCATCCTG AACAAGAAAA TTGTCCTTCA 
GCTCATTCTC CATTCCCAGA TGGTGATGCA GTGAGAAGGG AACTGAAGAG GCTGCAAGAA 
GTACACAATC AAGCCATTGG CAACAAGATT CAAGGACACG TTTCTGCAGA AAAGGCAAGA 
CAGGTTGAAG AGTTTTTGCA ACGGAAGCAT GAAGCAATGC AAAATAAGGC TCGAGCAGAG 
GGACATATGG ACGCAGTGCA CAACCTGGCA GCTATCTGTG GAGGCAGGCC AAATTCTGCA 
GGAGGAGGCA AGGCACGCAA CAGAGAAGAA AAGGAGTATT TAGCAAGACT TAGACAAATC 
AGACTCCAGA ATTTTAATGA GCGACAACAA ATAAAAGCCA AGCTGAGAGG GGCAAAGGGG 
GATGAAGGTG ACGCAGATGG TAAGGAAAGC AGTGAAGACG CTGAACTAAG AAGAAAGAAA 
ATTGAAGCTT TGAAGGCTCA AGCAAATGCA CGAGCTGCTT TAATAAAAGA AGAACTTGAG 
AGAAAGAGAA GAGAAGCAGA TAAAAGAGAG AAGAGACTAT GGGAAGAGCA TCTGGCGTTA 
AAAAATCCTA ATGTGCCTTG TAATGCAGTT GGGGCAGCAC CTGGTTCCCC CAAACATTCA 
CATCTTGACG TTGGGGGATC ACCTGTCCGA GGGCATCCTC ATGCGAAACC CAGCACACCT 
GCCATATCTA TGACTTCAGC GCTAAGGAAT GTTGGGGCTG AAGGCGAAGA TACAGGTATC 
AGGGATCCAA CAGAGGGCTC CAAAACATCA GACACCTCAG TACAAAATGA AAAGCGGCAA 
ATACTCCGCC GATTAAATAA TAACATGAAA TCTCAGGAAG AAACAGAAGC CACAAACCCG 
CCAACAGAGG AAAATGGAGT GCAAACCAAT GGCGAGGAAC AGCAAGAGAG TGGTGAGCCT 
CTGGCAGTAG ACAGAAAGAA GTGGCAGGCC GAGCCTCCTC CTGTTATCCC TCTTTCCCAG 
CTCACACTTG TAGAGGATAC AGTGTTCCCA TTGGACAATC TAACAGTTGG AGATGTCATC 
AAACTGGATG TGGAAGAACC CCAGAGACGA CTGTGGGGGA AAGATCCTGG TGATTCTGTG 
TTAAAGGTAC TCGGTGAGGC CAAACTCCAG CTTCAAACAG AATTGCTTCA AGTAACAATC 
GATGAACAGT GCCATGAAGA AGCAGATGAA GAGAAGGAAA ACCCTGACAT GCGGGACAAT 
TTGTTAAATA AAGTTTCACC CACTGAGGGA AAGGCTTTAA TTTTGCCTAA TAGAGAGGGA 
ATGCCACAGC CAGAAGTGTC TCCACTTGTA GAAGAGGAAT CCTCCGTGCC CCTTGTTGCC 
TTTAACCAAG TAGAGATCCA GTCTAATGAA GATCCTGAAG ATTTGGAGAC TGAGATACTT 
GAAGAAACAG AAGGCAAGGA GAAAGAGGGC AATGAGTGCC CAGTCCACGT TACAAATGTT 
TGGACTGAGC AGACCAAACA AGAAAGTGAG GGACAGGACC CTGTTGAATG CGAGCAACCA 
AATGAAGATG CCAAACTGGA TACTGAATGC TCAGAACAAG AGCAAAATAT AGAGAATCAG 
TCAAATAGAG AGTCAAGTGT ACCCAAAGCT TTATTTGAAA AGGTGCCACT CCCTCAAAGT 
GTAGTCACTC CAACATTCTC TGCCCAGTCA TCCTTCGATG ATTCCTTACC GCCTCGTTCT 
CGATCAGCTT CTCCAACCAA AACCAAAGGC AAATCAGCAC TGCTGATTGG ACTCTCTGCT 
GGTTTATTTG ATGCAAACAA CCCAAAGATG CTGCGCACAT GTTCGTTGCC CGACCTTAGC 
AAATTATTCA AGACCTTTGT GGATGAGCCC ACAGTGGCTG ATGTAGAAGG GGAGGAGCAG 
TTGGAAATAG AAGATATAGA GGATGAACCT GTTCAGGATG CGGATCACTC TGATTCAGAG 
GACACCATGT TTGGGGAGAC AGACCGAGAT TTACAGGAAA TGCAGGCATC AATGGAACAA 
CTATTGAGAG AGGAGCCTAC GGAAGATTAC TGCGCAGAGA AAGCAACCCA TTTAAACTCA 
GAACCACCAG CAGGAATCAA AGAGGAAAAT ATTGAGTCTG CCATTATTAA TGGGGCAGAG 
GAGGAGGAGG AGGAAGATGA AGAAGAAGAA GATGGAAATC AGCCAAGTGG TGAAAGTGTC 
ATTAATGAAG AATGGCATTC GGATGATAGC AGTGAGGACT TTGCCAGTGA ATGTGATGAT 
GGTGATAGCC TTTTTGCCCA TCTGGAAGAA ATGAGGTTTA AACTGGAACA AGAGATTGGT 
TTTGAAAAAT TCATAGAAGT CTACAATAAA ATCAAGGCTA TACACGAAGA TGAGGATGAA 
AATATTGATA GTTGTTCAAC AGTCGTGGAG AATATTCTAG GCTGTGAGCA CCAGCATTTG 
TATCCCAGAG TCCTTCGCCT AGTGATGGCA GATGGAGCTT ATCAAGAAGA TAATGACGAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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nek1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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