NEK1 gene cDNA ORF clone, Gallus gallus(chicken)

mRNA	Protein	Name
XM_015285374.2	XP_015140860.1	serine/threonine-protein kinase Nek1 isoform X1
XM_015285375.1	XP_015140861.1	serine/threonine-protein kinase Nek1 isoform X1
XM_015285382.1	XP_015140868.1	serine/threonine-protein kinase Nek1 isoform X6
XM_015285381.2	XP_015140867.1	serine/threonine-protein kinase Nek1 isoform X6
XM_015285378.1	XP_015140864.1	serine/threonine-protein kinase Nek1 isoform X2
XM_015285379.1	XP_015140865.1	serine/threonine-protein kinase Nek1 isoform X3
XM_015285376.1	XP_015140862.1	serine/threonine-protein kinase Nek1 isoform X1
XM_015285381.1	XP_015140867.1	serine/threonine-protein kinase Nek1 isoform X5
XM_015285378.2	XP_015140864.1	serine/threonine-protein kinase Nek1 isoform X2
XM_015285382.2	XP_015140868.1	serine/threonine-protein kinase Nek1 isoform X7
XM_420401.5	XP_420401.3	serine/threonine-protein kinase Nek1 isoform X1
XM_420401.6	XP_420401.3	serine/threonine-protein kinase Nek1 isoform X1
XM_025150496.1	XP_025006264.1	serine/threonine-protein kinase Nek1 isoform X4
XM_015285374.1	XP_015140860.1	serine/threonine-protein kinase Nek1 isoform X1
XM_015285380.1	XP_015140866.1	serine/threonine-protein kinase Nek1 isoform X4
XM_015285383.1	XP_015140869.1	serine/threonine-protein kinase Nek1 isoform X7
XM_015285376.2	XP_015140862.1	serine/threonine-protein kinase Nek1 isoform X1
XM_015285375.2	XP_015140861.1	serine/threonine-protein kinase Nek1 isoform X1
XM_015285379.2	XP_015140865.1	serine/threonine-protein kinase Nek1 isoform X3
XM_015285380.2	XP_015140866.1	serine/threonine-protein kinase Nek1 isoform X5
XM_015285383.2	XP_015140869.1	serine/threonine-protein kinase Nek1 isoform X8

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa150710	XM_025150496.1 Latest version!	Gallus gallus NIMA related kinase 1 (NEK1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OGa34987	XM_015285378.2 Latest version!	Gallus gallus NIMA related kinase 1 (NEK1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OGa34988	XM_015285380.2 Latest version!	Gallus gallus NIMA related kinase 1 (NEK1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OGa34989	XM_015285383.2 Latest version!	Gallus gallus NIMA related kinase 1 (NEK1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OGa07767	XM_015285375.2 Latest version!	Gallus gallus NIMA related kinase 1 (NEK1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OGa07767	XM_015285374.2 Latest version!	Gallus gallus NIMA related kinase 1 (NEK1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OGa07767	XM_420401.6 Latest version!	Gallus gallus NIMA related kinase 1 (NEK1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OGa07767	XM_015285376.2 Latest version!	Gallus gallus NIMA related kinase 1 (NEK1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OGa07768	XM_015285379.2 Latest version!	Gallus gallus NIMA related kinase 1 (NEK1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OGa07769	XM_015285381.2 Latest version!	Gallus gallus NIMA related kinase 1 (NEK1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OGa07770	XM_015285382.2 Latest version!	Gallus gallus NIMA related kinase 1 (NEK1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
OGa07769	XM_015285381.1	Gallus gallus NIMA-related kinase 1 (NEK1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OGa34987	XM_015285378.1	Gallus gallus NIMA-related kinase 1 (NEK1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OGa07770	XM_015285382.1	Gallus gallus NIMA-related kinase 1 (NEK1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OGa07767	XM_420401.5	Gallus gallus NIMA-related kinase 1 (NEK1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OGa07767	XM_015285374.1	Gallus gallus NIMA-related kinase 1 (NEK1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OGa07767	XM_015285376.1	Gallus gallus NIMA-related kinase 1 (NEK1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OGa07767	XM_015285375.1	Gallus gallus NIMA-related kinase 1 (NEK1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OGa07768	XM_015285379.1	Gallus gallus NIMA-related kinase 1 (NEK1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OGa34988	XM_015285380.1	Gallus gallus NIMA-related kinase 1 (NEK1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OGa34989	XM_015285383.1	Gallus gallus NIMA-related kinase 1 (NEK1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
Hide Old Accession Versions >>

NEK1 ( XM_025150496.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025006264.1 Gallus gallus NIMA related kinase 1 (NEK1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OGa150710
Clone ID Related Accession (Same CDS sequence)	XM_025150496.1
Accession Version	XM_025150496.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3615bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	serine/threonine-protein kinase Nek1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAAAT ACATTAAAGT GCAAAAAATT GGAGAAGGAT CCTTTGGGAA AGCATTTCTT 
GTTAAAGATA AAGAAAATAG CCAGCAGTAT GTTATTAAAG AAATTAACAT ATCTAAGATG 
TCAAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TACTGGCCAA CATGAAACAT 
CCAAATATTG TCCTGTATAG GGAGTCGTTT GAAGAAAATG GTTGTCTCTA CATAGTGATG 
GATTACTGTG AAGGAGGAGA TCTGTTCAAA AAAATAAATG CCCAGAAAGG AGTCCTGTTC 
TCTGAGGATC AGATTCTTGA TTGGTTTGTA CAGATCTGTT TGGCACTGAA ACATATACAT 
GATAGAAAAA TCTTACATCG CGACATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA 
ACAATACAGC TGGGAGATTT TGGAATCGCG AGAGTTCTGA ACAGTGATAT CTGGGCCCTT 
GGTTGCGTTC TCTATGAAAT GTGCACACTT AAGCATGCAT TTGAAGCTGG TAACATGAAG 
AACTTGGTAC TGAAGATAAT CTCTGGATCT TTCCCTCCTG TTTCTGTGCA TTATTCCTAT 
GACCTACGTA ATCTACTGTC ACAGTTATTT AAAAGGAATC CTAGGGATAG ACCATCAGTG 
AACTCCATAT TGGAGAAAAA TTTCATAGCC AAACGGGTTG AAAAATTTCT CACACCGCAG 
CTTATTGCAG AAGAATTCAG TCACAAAGTG TTTCACAAGT TTGGACCACA TGCTGGACCA 
GCTAAAAGAC CAGCTCAAGG ACATATCTCG GCTTCAATTG CACCAGTGCA GAAAATTACC 
AAACCTGCTG CAAAATACGG AGTGCCTTTA GCGATGAAGA AGTGTTATGA CGCACCCAAA 
AAGCTTCATG AGAAAAAGTC ATTGATTAAA CTTAGGCAGG CCTTTCCAAC TCCAAAGAAG 
AAAATTATTC CAGGAGAAGA AAGGAGGAAA ATGTTTGAGG AACCTTCAAA AAAGAAAAGG 
TTAGAATTGC CTGAAAAAGA AAAACGCCAG AGAGATCAGA TCAGTTTACT GAAGGCAGAG 
GAAATGAGAA GATTGGAAAA AGAAAGGATG GAACGAATAA ACAGAGCCAG GGAGCAAGGA 
TGGAGGAATG TGCTTGGTTC AGGTGGAAGT GGTGATGTTA AGGCACCATA TTATGGCGGT 
GGAGGTGGTG TTGGTCCTTT TCCTGCCTCT TTCAGAGGAC AGTATGAGCA CTATCATGCT 
ATTTTTGACC AGATGCAAAA GGAAACCTTC AAAGTAACTA AAGAGAGGCA AGCCAAATGG 
AGGGCGGAGG TACAAGGCCA AGAAGCTGTT GAGAGAGGAA TTCCACCTGG AGTACGTCCA 
GGAGTTCCTA AGGGGCCTGC AGGTCATCAC CATTTACCTG ATGCTGATGC AATTAGGAAA 
AAAGTGAAAA GGTTGAAGGA GGTGTCTAAA CAAGCCAGTG CAAACAGGCA GAAAGGATGG 
CTACCTGCAG AAAGAGCAAA GCAAGTTGAA GAATTCTGGC AACGAAAGAG GGAAGCCATG 
CAAAACAAAG CTCGTGCTGA GGGACACATG GGTACACTGC AAAACGTGGC AGATATCTAT 
GGAGGCAGGC CCATGTCTGC AAGAGGAAGG AAATCCAGAA ACAAGGAAGA AGAGGAATAT 
TTGGCAAGAT TAAGACAGAT ACGACTACAA AACTTCAATG AGCGTCAACA GATTAAAGCA 
AAACTTCGTG GAGAGAAGAA TGAAGCTCCC AGTTCTGATG GACAAGAGTC AAGTGAGGAA 
GCAGAAATGA AACGCAAAAA GATAGAAGCA CAGAAGGCCC AAGCAAATGC TCGAGCTGCA 
GTTCTGAAAG AACAGTTAGA GCGAAAGAGG AAGGAGGCAT ATGAAAGAGA GAAAAGAGCT 
TGGGAAGAAC ATCTGATAGC AAAAGGAGTA AAGAATCCTA ATGTGCCTTT TCATGTGGGA 
GCAGCAGGAC ACACTCCTGT GCATGGAATA CAAGAGCCTG GAGTGTCTCC TCCTAAGCAA 
CAGCTTCCAG TTAAGCCCAT CTCCATGACT TCTGCTTTGA AAGAAGTTGG CGTGGATGGA 
AATGTAACCG CTGCCCAGGA GGTTAAAGAG GAAATAAAAA AGCCAGATCT TACAATGCAG 
AACAAGAGGC AGATACTGAA AAGATTGAAT CAAAATCTTA AAGCTCAGGA AGATGAGAAA 
GGAAGAAAGG ACGATAAAAA TATCTCTGAG TCAGATGGGT CTGAAGATGG TAAAGAAGAG 
CAAGAAGATA ACCATCCACT TCTGGGAGAT CGCAAAAGAT GGGCATCAAG GGCATCAGAT 
TTGGTAGTTC CTTTAGCTCA GTTATCAATG GAAGATTCTC TCTCTGGAAC AGACCGGCAA 
ACTATGGGGG AAGTAATCAG GCTAGATATT GGGGAACCCC GCAGGAAAGT CTGGGGCAAA 
AGCCCTACTG ATTCGGTCCT GAAGATTCTA GGAGAAGCGG AGTTGCAGCT ACCAACTGAA 
TTACTGGAAG AAATAGATGT AATAAATGAG CCTGATGATT TGGAAGCAGT GATGTTGAAA 
GAAACAGAAG AAGATAAAAA GCACCAGAGC AAGGAATTTC CCATTGCTGT TAATGAAGTG 
TGGGTGAAAG AAAAACAGGA TGAGGCACAA CAGGATGCAA GAAATGAAGC AGCTTTTGAG 
AAAGAAGTAG TGCTTGATGA AGTGCAATCA CAAAATGAAG CTCCAGAAGA AACTTGTTCC 
AGTGACTCTC TGCAACCTGA ACCGTTATTC CAGAGAGTAA TACAGCCCCC AGCTGTACCT 
ACAGCCTCAC CAGTTCTATC ATCTCAGTCT TCCCAAGAAG AGCCTTTTGT ACCTCGTTCA 
CGTTCCATAT CGCCAGCAAA AAATAAAGGG AAAAGTTCAT TGTTTTTTGG ACTTTCTACA 
GGTCTGTTTG ATGCAAACAA CCCAAAGATG TTGAGAACGT GTTCACTCCC GGATCTTTAC 
AAACTGTACA GAACCTTAGT TGATGTTCCC AGCATAGCAG ATGTACAGCA TCAACACAGT 
CTTGAAATAG ATGATACGGA AGATGAGCAA GCCAAAGAAG GACCCTCTGA TTCTGAGGAT 
ATCATGTTTG GGGACGCCGA TACAGATTTG CAGGAACTCC GGGCCTCAAT GGAACAATTA 
CTTAGGGAGC AGCCTAGTGA GGAATTAAGT GAAGAGGAGG AATCTACTTT AAAGGCTAAC 
GTCATCAAAT GCATGACAAA TGGTACAGAG CTGGATGAAG CAGATGAAAA TAACCCCAGC 
AGTGAAAGTG CACTTAATGA AGAATGGCAG TCAGATAATA GTGATGGAGA TATTGCCAGT 
GAATGTGAAG AATGTGATAG TGTCTTCAGC CATTTGGAAG AGCTGAGATA CAACCTGGAA 
CAGGAAATAG GCTTTGAGAA ATTAATTGAA GTTTATAATA AAATAAAGGC CATACATGAA 
GATGAAGATG AAAATATTGA TATTTGTTCA AGCATAGTTC AGACTATTCT GGGAAATGAA 
CACAAACACT TATATGCCAA AGTACTTCAC CTGGTCATGG CGGATGGAGC CTACCAGGAA 
GATAATGACG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025006264.1
CDS	1005..4619
Translation	MDKYIKVQKIGEGSFGKAFLVKDKENSQQYVIKEINISKMSNKEREESRREVAVLANMKHPNIVLYRESFEENGCLYIVMDYCEGGDLFKKINAQKGVLFSEDQILDWFVQICLALKHIHDRKILHRDIKSQNIFLTKDGTIQLGDFGIARVLNSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIISGSFPPVSVHYSYDLRNLLSQLFKRNPRDRPSVNSILEKNFIAKRVEKFLTPQLIAEEFSHKVFHKFGPHAGPAKRPAQGHISASIAPVQKITKPAAKYGVPLAMKKCYDAPKKLHEKKSLIKLRQAFPTPKKKIIPGEERRKMFEEPSKKKRLELPEKEKRQRDQISLLKAEEMRRLEKERMERINRAREQGWRNVLGSGGSGDVKAPYYGGGGGVGPFPASFRGQYEHYHAIFDQMQKETFKVTKERQAKWRAEVQGQEAVERGIPPGVRPGVPKGPAGHHHLPDADAIRKKVKRLKEVSKQASANRQKGWLPAERAKQVEEFWQRKREAMQNKARAEGHMGTLQNVADIYGGRPMSARGRKSRNKEEEEYLARLRQIRLQNFNERQQIKAKLRGEKNEAPSSDGQESSEEAEMKRKKIEAQKAQANARAAVLKEQLERKRKEAYEREKRAWEEHLIAKGVKNPNVPFHVGAAGHTPVHGIQEPGVSPPKQQLPVKPISMTSALKEVGVDGNVTAAQEVKEEIKKPDLTMQNKRQILKRLNQNLKAQEDEKGRKDDKNISESDGSEDGKEEQEDNHPLLGDRKRWASRASDLVVPLAQLSMEDSLSGTDRQTMGEVIRLDIGEPRRKVWGKSPTDSVLKILGEAELQLPTELLEEIDVINEPDDLEAVMLKETEEDKKHQSKEFPIAVNEVWVKEKQDEAQQDARNEAAFEKEVVLDEVQSQNEAPEETCSSDSLQPEPLFQRVIQPPAVPTASPVLSSQSSQEEPFVPRSRSISPAKNKGKSSLFFGLSTGLFDANNPKMLRTCSLPDLYKLYRTLVDVPSIADVQHQHSLEIDDTEDEQAKEGPSDSEDIMFGDADTDLQELRASMEQLLREQPSEELSEEEESTLKANVIKCMTNGTELDEADENNPSSESALNEEWQSDNSDGDIASECEECDSVFSHLEELRYNLEQEIGFEKLIEVYNKIKAIHEDEDENIDICSSIVQTILGNEHKHLYAKVLHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_025150496.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus NIMA related kinase 1 (NEK1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025150496.1

ORF Insert Sequence:

ATGGATAAAT ACATTAAAGT GCAAAAAATT GGAGAAGGAT CCTTTGGGAA AGCATTTCTT 
GTTAAAGATA AAGAAAATAG CCAGCAGTAT GTTATTAAAG AAATTAACAT ATCTAAGATG 
TCAAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TACTGGCCAA CATGAAACAT 
CCAAATATTG TCCTGTATAG GGAGTCGTTT GAAGAAAATG GTTGTCTCTA CATAGTGATG 
GATTACTGTG AAGGAGGAGA TCTGTTCAAA AAAATAAATG CCCAGAAAGG AGTCCTGTTC 
TCTGAGGATC AGATTCTTGA TTGGTTTGTA CAGATCTGTT TGGCACTGAA ACATATACAT 
GATAGAAAAA TCTTACATCG CGACATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA 
ACAATACAGC TGGGAGATTT TGGAATCGCG AGAGTTCTGA ACAGTGATAT CTGGGCCCTT 
GGTTGCGTTC TCTATGAAAT GTGCACACTT AAGCATGCAT TTGAAGCTGG TAACATGAAG 
AACTTGGTAC TGAAGATAAT CTCTGGATCT TTCCCTCCTG TTTCTGTGCA TTATTCCTAT 
GACCTACGTA ATCTACTGTC ACAGTTATTT AAAAGGAATC CTAGGGATAG ACCATCAGTG 
AACTCCATAT TGGAGAAAAA TTTCATAGCC AAACGGGTTG AAAAATTTCT CACACCGCAG 
CTTATTGCAG AAGAATTCAG TCACAAAGTG TTTCACAAGT TTGGACCACA TGCTGGACCA 
GCTAAAAGAC CAGCTCAAGG ACATATCTCG GCTTCAATTG CACCAGTGCA GAAAATTACC 
AAACCTGCTG CAAAATACGG AGTGCCTTTA GCGATGAAGA AGTGTTATGA CGCACCCAAA 
AAGCTTCATG AGAAAAAGTC ATTGATTAAA CTTAGGCAGG CCTTTCCAAC TCCAAAGAAG 
AAAATTATTC CAGGAGAAGA AAGGAGGAAA ATGTTTGAGG AACCTTCAAA AAAGAAAAGG 
TTAGAATTGC CTGAAAAAGA AAAACGCCAG AGAGATCAGA TCAGTTTACT GAAGGCAGAG 
GAAATGAGAA GATTGGAAAA AGAAAGGATG GAACGAATAA ACAGAGCCAG GGAGCAAGGA 
TGGAGGAATG TGCTTGGTTC AGGTGGAAGT GGTGATGTTA AGGCACCATA TTATGGCGGT 
GGAGGTGGTG TTGGTCCTTT TCCTGCCTCT TTCAGAGGAC AGTATGAGCA CTATCATGCT 
ATTTTTGACC AGATGCAAAA GGAAACCTTC AAAGTAACTA AAGAGAGGCA AGCCAAATGG 
AGGGCGGAGG TACAAGGCCA AGAAGCTGTT GAGAGAGGAA TTCCACCTGG AGTACGTCCA 
GGAGTTCCTA AGGGGCCTGC AGGTCATCAC CATTTACCTG ATGCTGATGC AATTAGGAAA 
AAAGTGAAAA GGTTGAAGGA GGTGTCTAAA CAAGCCAGTG CAAACAGGCA GAAAGGATGG 
CTACCTGCAG AAAGAGCAAA GCAAGTTGAA GAATTCTGGC AACGAAAGAG GGAAGCCATG 
CAAAACAAAG CTCGTGCTGA GGGACACATG GGTACACTGC AAAACGTGGC AGATATCTAT 
GGAGGCAGGC CCATGTCTGC AAGAGGAAGG AAATCCAGAA ACAAGGAAGA AGAGGAATAT 
TTGGCAAGAT TAAGACAGAT ACGACTACAA AACTTCAATG AGCGTCAACA GATTAAAGCA 
AAACTTCGTG GAGAGAAGAA TGAAGCTCCC AGTTCTGATG GACAAGAGTC AAGTGAGGAA 
GCAGAAATGA AACGCAAAAA GATAGAAGCA CAGAAGGCCC AAGCAAATGC TCGAGCTGCA 
GTTCTGAAAG AACAGTTAGA GCGAAAGAGG AAGGAGGCAT ATGAAAGAGA GAAAAGAGCT 
TGGGAAGAAC ATCTGATAGC AAAAGGAGTA AAGAATCCTA ATGTGCCTTT TCATGTGGGA 
GCAGCAGGAC ACACTCCTGT GCATGGAATA CAAGAGCCTG GAGTGTCTCC TCCTAAGCAA 
CAGCTTCCAG TTAAGCCCAT CTCCATGACT TCTGCTTTGA AAGAAGTTGG CGTGGATGGA 
AATGTAACCG CTGCCCAGGA GGTTAAAGAG GAAATAAAAA AGCCAGATCT TACAATGCAG 
AACAAGAGGC AGATACTGAA AAGATTGAAT CAAAATCTTA AAGCTCAGGA AGATGAGAAA 
GGAAGAAAGG ACGATAAAAA TATCTCTGAG TCAGATGGGT CTGAAGATGG TAAAGAAGAG 
CAAGAAGATA ACCATCCACT TCTGGGAGAT CGCAAAAGAT GGGCATCAAG GGCATCAGAT 
TTGGTAGTTC CTTTAGCTCA GTTATCAATG GAAGATTCTC TCTCTGGAAC AGACCGGCAA 
ACTATGGGGG AAGTAATCAG GCTAGATATT GGGGAACCCC GCAGGAAAGT CTGGGGCAAA 
AGCCCTACTG ATTCGGTCCT GAAGATTCTA GGAGAAGCGG AGTTGCAGCT ACCAACTGAA 
TTACTGGAAG AAATAGATGT AATAAATGAG CCTGATGATT TGGAAGCAGT GATGTTGAAA 
GAAACAGAAG AAGATAAAAA GCACCAGAGC AAGGAATTTC CCATTGCTGT TAATGAAGTG 
TGGGTGAAAG AAAAACAGGA TGAGGCACAA CAGGATGCAA GAAATGAAGC AGCTTTTGAG 
AAAGAAGTAG TGCTTGATGA AGTGCAATCA CAAAATGAAG CTCCAGAAGA AACTTGTTCC 
AGTGACTCTC TGCAACCTGA ACCGTTATTC CAGAGAGTAA TACAGCCCCC AGCTGTACCT 
ACAGCCTCAC CAGTTCTATC ATCTCAGTCT TCCCAAGAAG AGCCTTTTGT ACCTCGTTCA 
CGTTCCATAT CGCCAGCAAA AAATAAAGGG AAAAGTTCAT TGTTTTTTGG ACTTTCTACA 
GGTCTGTTTG ATGCAAACAA CCCAAAGATG TTGAGAACGT GTTCACTCCC GGATCTTTAC 
AAACTGTACA GAACCTTAGT TGATGTTCCC AGCATAGCAG ATGTACAGCA TCAACACAGT 
CTTGAAATAG ATGATACGGA AGATGAGCAA GCCAAAGAAG GACCCTCTGA TTCTGAGGAT 
ATCATGTTTG GGGACGCCGA TACAGATTTG CAGGAACTCC GGGCCTCAAT GGAACAATTA 
CTTAGGGAGC AGCCTAGTGA GGAATTAAGT GAAGAGGAGG AATCTACTTT AAAGGCTAAC 
GTCATCAAAT GCATGACAAA TGGTACAGAG CTGGATGAAG CAGATGAAAA TAACCCCAGC 
AGTGAAAGTG CACTTAATGA AGAATGGCAG TCAGATAATA GTGATGGAGA TATTGCCAGT 
GAATGTGAAG AATGTGATAG TGTCTTCAGC CATTTGGAAG AGCTGAGATA CAACCTGGAA 
CAGGAAATAG GCTTTGAGAA ATTAATTGAA GTTTATAATA AAATAAAGGC CATACATGAA 
GATGAAGATG AAAATATTGA TATTTGTTCA AGCATAGTTC AGACTATTCT GGGAAATGAA 
CACAAACACT TATATGCCAA AGTACTTCAC CTGGTCATGG CGGATGGAGC CTACCAGGAA 
GATAATGACG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_015285378.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015140864.1 Gallus gallus NIMA-related kinase 1 (NEK1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OGa34987
Clone ID Related Accession (Same CDS sequence)	XM_015285378.1 , XM_015285378.2
Accession Version	XM_015285378.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3642bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	serine/threonine-protein kinase Nek1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455855.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAAAT ACATTAAAGT GCAAAAAATT GGAGAAGGAT CCTTTGGGAA AGCATTTCTT 
GTTAAAGATA AAGAAAATAG CCAGCAGTAT GTTATTAAAG AAATTAACAT ATCTAAGATG 
TCAAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TACTGGCCAA CATGAAACAT 
CCAAATATTG TCCTGTATAG GGAGTCGTTT GAAGAAAATG GTTGTCTCTA CATAGTGATG 
GATTACTGTG AAGGAGGAGA TCTGTTCAAA AAAATAAATG CCCAGAAAGG AGTCCTGTTC 
TCTGAGGATC AGATTCTTGA TTGGTTTGTA CAGATCTGTT TGGCACTGAA ACATATACAT 
GATAGAAAAA TCTTACATCG CGACATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA 
ACAATACAGC TGGGAGATTT TGGAATCGCG AGAGTTCTGA ACAGTACTGT GGAATTGGCT 
CGCACTTGCA TAGGAACACC ATACTATTTA TCACCTGAAA TATGCCAGAA CAGGCCTTAC 
AACAATAAAA GTGATATCTG GGCCCTTGGT TGCGTTCTCT ATGAAATGTG CACACTTAAG 
CATGCATTTG AAGCTGGTAA CATGAAGAAC TTGGTACTGA AGATAATCTC TGGATCTTTC 
CCTCCTGTTT CTGTGCATTA TTCCTATGAC CTACGTAATC TACTGTCACA GTTATTTAAA 
AGGAATCCTA GGGATAGACC ATCAGTGAAC TCCATATTGG AGAAAAATTT CATAGCCAAA 
CGGGTTGAAA AATTTCTCAC ACCGCAGCTT ATTGCAGAAG AATTCAGTCA CAAAGTGTTT 
CACAAGTTTG GACCACATGC TGGACCAGCT AAAAGACCAG CTCAAGGACA TATCTCGGCT 
TCAATTGCAC CAGTGCAGAA AATTACCAAA CCTGCTGCAA AATACGGAGT GCCTTTAGCG 
ATGAAGAAGT GTTATGACGC ACCCAAAAAG CTTCATGAGA AAAAGTCATT GATTAAACTT 
AGGCAGGAAC CTTCAAAAAA GAAAAGGTTA GAATTGCCTG AAAAAGAAAA ACGCCAGAGA 
GATCAGATCA GTTTACTGAA GGCAGAGGAA ATGAGAAGAT TGGAAAAAGA AAGGATGGAA 
CGAATAAACA GAGCCAGGGA GCAAGGATGG AGGAATGTGC TTGGTTCAGG TGGAAGTGGT 
GATGTTAAGG CACCATATTA TGGCGGTGGA GGTGGTGTTG GTCCTTTTCC TGCCTCTTTC 
AGAGGACAGT ATGAGCACTA TCATGCTATT TTTGACCAGA TGCAAAAGGA AACCTTCAAA 
GTAACTAAAG AGAGGCAAGC CAAATGGAGG GCGGAGGTAC AAGGCCAAGA AGCTGTTGAG 
AGAGGAATTC CACCTGGAGT ACGTCCAGGA GTTCCTAAGG GGCCTGCAGG TCATCACCAT 
TTACCTGATG CTGATGCAAT TAGGAAAAAA GTGAAAAGGT TGAAGGAGGT GTCTAAACAA 
GCCAGTGCAA ACAGGCAGAA AGGATGGCTA CCTGCAGAAA GAGCAAAGCA AGTTGAAGAA 
TTCTGGCAAC GAAAGAGGGA AGCCATGCAA AACAAAGCTC GTGCTGAGGG ACACATGGGT 
ACACTGCAAA ACGTGGCAGA TATCTATGGA GGCAGGCCCA TGTCTGCAAG AGGAAGGAAA 
TCCAGAAACA AGGAAGAAGA GGAATATTTG GCAAGATTAA GACAGATACG ACTACAAAAC 
TTCAATGAGC GTCAACAGAT TAAAGCAAAA CTTCGTGGAG AGAAGAATGA AGCTCCCAGT 
TCTGATGGAC AAGAGTCAAG TGAGGAAGCA GAAATGAAAC GCAAAAAGAT AGAAGCACAG 
AAGGCCCAAG CAAATGCTCG AGCTGCAGTT CTGAAAGAAC AGTTAGAGCG AAAGAGGAAG 
GAGGCATATG AAAGAGAGAA AAGAGCTTGG GAAGAACATC TGATAGCAAA AGGAGTAAAG 
AATCCTAATG TGCCTTTTCA TGTGGGAGCA GCAGGACACA CTCCTGTGCA TGGAATACAA 
GAGCCTGGAG TGTCTCCTCC TAAGCAACAG CTTCCAGTTA AGCCCATCTC CATGACTTCT 
GCTTTGAAAG AAGTTGGCGT GGATGGAAAT GTAACCGCTG CCCAGGAGGT TAAAGAGGAA 
ATAAAAAAGC CAGATCTTAC AATGCAGAAC AAGAGGCAGA TACTGAAAAG ATTGAATCAA 
AATCTTAAAG CTCAGGAAGA TGAGAAAGGA AGAAAGGACG ATAAAAATAT CTCTGAGTCA 
GATGGGTCTG AAGATGGTAA AGAAGAGCAA GAAGATAACC ATCCACTTCT GGGAGATCGC 
AAAAGATGGG CATCAAGGGC ATCAGATTTG GTAGTTCCTT TAGCTCAGTT ATCAATGGAA 
GATTCTCTCT CTGGAACAGA CCGGCAAACT ATGGGGGAAG TAATCAGGCT AGATATTGGG 
GAACCCCGCA GGAAAGTCTG GGGCAAAAGC CCTACTGATT CGGTCCTGAA GATTCTAGGA 
GAAGCGGAGT TGCAGCTACC AACTGAATTA CTGGAAGAAA TAGATGTAAT AAATGAGCCT 
GATGATTTGG AAGCAGTGAT GTTGAAAGAA ACAGAAGAAG ATAAAAAGCA CCAGAGCAAG 
GAATTTCCCA TTGCTGTTAA TGAAGTGTGG GTGAAAGAAA AACAGGATGA GGCACAACAG 
GATGCAAGAA ATGAAGCAGC TTTTGAGAAA GAAGTAGTGC TTGATGAAGT GCAATCACAA 
AATGAAGCTC CAGAAGAAAC TTGTTCCAGT GACTCTCTGC AACCTGAACC GTTATTCCAG 
AGAGTAATAC AGCCCCCAGC TGTACCTACA GCCTCACCAG TTCTATCATC TCAGTCTTCC 
CAAGAAGAGC CTTTTGTACC TCGTTCACGT TCCATATCGC CAGCAAAAAA TAAAGGGAAA 
AGTTCATTGT TTTTTGGACT TTCTACAGGT CTGTTTGATG CAAACAACCC AAAGATGTTG 
AGAACGTGTT CACTCCCGGA TCTTTACAAA CTGTACAGAA CCTTAGTTGA TGTTCCCAGC 
ATAGCAGATG TACAGCATCA ACACAGTCTT GAAATAGATG ATACGGAAGA TGAGCAAGCC 
AAAGAAGGAC CCTCTGATTC TGAGGATATC ATGTTTGGGG ACGCCGATAC AGATTTGCAG 
GAACTCCGGG CCTCAATGGA ACAATTACTT AGGGAGCAGC CTAGTGAGGA ATTAAGTGAA 
GAGGAGGAAT CTACTTTAAA GGCTAACGTC ATCAAATGCA TGACAAATGG TACAGAGCTG 
GATGAAGCAG ATGAAAATAA CCCCAGCAGT GAAAGTGCAC TTAATGAAGA ATGGCAGTCA 
GATAATAGTG ATGGAGATAT TGCCAGTGAA TGTGAAGAAT GTGATAGTGT CTTCAGCCAT 
TTGGAAGAGC TGAGATACAA CCTGGAACAG GAAATAGGCT TTGAGAAATT AATTGAAGTT 
TATAATAAAA TAAAGGCCAT ACATGAAGAT GAAGATGAAA ATATTGATAT TTGTTCAAGC 
ATAGTTCAGA CTATTCTGGG AAATGAACAC AAACACTTAT ATGCCAAAGT ACTTCACCTG 
GTCATGGCGG ATGGAGCCTA CCAGGAAGAT AATGACGAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015140864.1
CDS	1007..4648
Translation	MDKYIKVQKIGEGSFGKAFLVKDKENSQQYVIKEINISKMSNKEREESRREVAVLANMKHPNIVLYRESFEENGCLYIVMDYCEGGDLFKKINAQKGVLFSEDQILDWFVQICLALKHIHDRKILHRDIKSQNIFLTKDGTIQLGDFGIARVLNSTVELARTCIGTPYYLSPEICQNRPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIISGSFPPVSVHYSYDLRNLLSQLFKRNPRDRPSVNSILEKNFIAKRVEKFLTPQLIAEEFSHKVFHKFGPHAGPAKRPAQGHISASIAPVQKITKPAAKYGVPLAMKKCYDAPKKLHEKKSLIKLRQEPSKKKRLELPEKEKRQRDQISLLKAEEMRRLEKERMERINRAREQGWRNVLGSGGSGDVKAPYYGGGGGVGPFPASFRGQYEHYHAIFDQMQKETFKVTKERQAKWRAEVQGQEAVERGIPPGVRPGVPKGPAGHHHLPDADAIRKKVKRLKEVSKQASANRQKGWLPAERAKQVEEFWQRKREAMQNKARAEGHMGTLQNVADIYGGRPMSARGRKSRNKEEEEYLARLRQIRLQNFNERQQIKAKLRGEKNEAPSSDGQESSEEAEMKRKKIEAQKAQANARAAVLKEQLERKRKEAYEREKRAWEEHLIAKGVKNPNVPFHVGAAGHTPVHGIQEPGVSPPKQQLPVKPISMTSALKEVGVDGNVTAAQEVKEEIKKPDLTMQNKRQILKRLNQNLKAQEDEKGRKDDKNISESDGSEDGKEEQEDNHPLLGDRKRWASRASDLVVPLAQLSMEDSLSGTDRQTMGEVIRLDIGEPRRKVWGKSPTDSVLKILGEAELQLPTELLEEIDVINEPDDLEAVMLKETEEDKKHQSKEFPIAVNEVWVKEKQDEAQQDARNEAAFEKEVVLDEVQSQNEAPEETCSSDSLQPEPLFQRVIQPPAVPTASPVLSSQSSQEEPFVPRSRSISPAKNKGKSSLFFGLSTGLFDANNPKMLRTCSLPDLYKLYRTLVDVPSIADVQHQHSLEIDDTEDEQAKEGPSDSEDIMFGDADTDLQELRASMEQLLREQPSEELSEEEESTLKANVIKCMTNGTELDEADENNPSSESALNEEWQSDNSDGDIASECEECDSVFSHLEELRYNLEQEIGFEKLIEVYNKIKAIHEDEDENIDICSSIVQTILGNEHKHLYAKVLHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_015285378.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus NIMA-related kinase 1 (NEK1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015285378.1

ORF Insert Sequence:

ATGGATAAAT ACATTAAAGT GCAAAAAATT GGAGAAGGAT CCTTTGGGAA AGCATTTCTT 
GTTAAAGATA AAGAAAATAG CCAGCAGTAT GTTATTAAAG AAATTAACAT ATCTAAGATG 
TCAAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TACTGGCCAA CATGAAACAT 
CCAAATATTG TCCTGTATAG GGAGTCGTTT GAAGAAAATG GTTGTCTCTA CATAGTGATG 
GATTACTGTG AAGGAGGAGA TCTGTTCAAA AAAATAAATG CCCAGAAAGG AGTCCTGTTC 
TCTGAGGATC AGATTCTTGA TTGGTTTGTA CAGATCTGTT TGGCACTGAA ACATATACAT 
GATAGAAAAA TCTTACATCG CGACATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA 
ACAATACAGC TGGGAGATTT TGGAATCGCG AGAGTTCTGA ACAGTACTGT GGAATTGGCT 
CGCACTTGCA TAGGAACACC ATACTATTTA TCACCTGAAA TATGCCAGAA CAGGCCTTAC 
AACAATAAAA GTGATATCTG GGCCCTTGGT TGCGTTCTCT ATGAAATGTG CACACTTAAG 
CATGCATTTG AAGCTGGTAA CATGAAGAAC TTGGTACTGA AGATAATCTC TGGATCTTTC 
CCTCCTGTTT CTGTGCATTA TTCCTATGAC CTACGTAATC TACTGTCACA GTTATTTAAA 
AGGAATCCTA GGGATAGACC ATCAGTGAAC TCCATATTGG AGAAAAATTT CATAGCCAAA 
CGGGTTGAAA AATTTCTCAC ACCGCAGCTT ATTGCAGAAG AATTCAGTCA CAAAGTGTTT 
CACAAGTTTG GACCACATGC TGGACCAGCT AAAAGACCAG CTCAAGGACA TATCTCGGCT 
TCAATTGCAC CAGTGCAGAA AATTACCAAA CCTGCTGCAA AATACGGAGT GCCTTTAGCG 
ATGAAGAAGT GTTATGACGC ACCCAAAAAG CTTCATGAGA AAAAGTCATT GATTAAACTT 
AGGCAGGAAC CTTCAAAAAA GAAAAGGTTA GAATTGCCTG AAAAAGAAAA ACGCCAGAGA 
GATCAGATCA GTTTACTGAA GGCAGAGGAA ATGAGAAGAT TGGAAAAAGA AAGGATGGAA 
CGAATAAACA GAGCCAGGGA GCAAGGATGG AGGAATGTGC TTGGTTCAGG TGGAAGTGGT 
GATGTTAAGG CACCATATTA TGGCGGTGGA GGTGGTGTTG GTCCTTTTCC TGCCTCTTTC 
AGAGGACAGT ATGAGCACTA TCATGCTATT TTTGACCAGA TGCAAAAGGA AACCTTCAAA 
GTAACTAAAG AGAGGCAAGC CAAATGGAGG GCGGAGGTAC AAGGCCAAGA AGCTGTTGAG 
AGAGGAATTC CACCTGGAGT ACGTCCAGGA GTTCCTAAGG GGCCTGCAGG TCATCACCAT 
TTACCTGATG CTGATGCAAT TAGGAAAAAA GTGAAAAGGT TGAAGGAGGT GTCTAAACAA 
GCCAGTGCAA ACAGGCAGAA AGGATGGCTA CCTGCAGAAA GAGCAAAGCA AGTTGAAGAA 
TTCTGGCAAC GAAAGAGGGA AGCCATGCAA AACAAAGCTC GTGCTGAGGG ACACATGGGT 
ACACTGCAAA ACGTGGCAGA TATCTATGGA GGCAGGCCCA TGTCTGCAAG AGGAAGGAAA 
TCCAGAAACA AGGAAGAAGA GGAATATTTG GCAAGATTAA GACAGATACG ACTACAAAAC 
TTCAATGAGC GTCAACAGAT TAAAGCAAAA CTTCGTGGAG AGAAGAATGA AGCTCCCAGT 
TCTGATGGAC AAGAGTCAAG TGAGGAAGCA GAAATGAAAC GCAAAAAGAT AGAAGCACAG 
AAGGCCCAAG CAAATGCTCG AGCTGCAGTT CTGAAAGAAC AGTTAGAGCG AAAGAGGAAG 
GAGGCATATG AAAGAGAGAA AAGAGCTTGG GAAGAACATC TGATAGCAAA AGGAGTAAAG 
AATCCTAATG TGCCTTTTCA TGTGGGAGCA GCAGGACACA CTCCTGTGCA TGGAATACAA 
GAGCCTGGAG TGTCTCCTCC TAAGCAACAG CTTCCAGTTA AGCCCATCTC CATGACTTCT 
GCTTTGAAAG AAGTTGGCGT GGATGGAAAT GTAACCGCTG CCCAGGAGGT TAAAGAGGAA 
ATAAAAAAGC CAGATCTTAC AATGCAGAAC AAGAGGCAGA TACTGAAAAG ATTGAATCAA 
AATCTTAAAG CTCAGGAAGA TGAGAAAGGA AGAAAGGACG ATAAAAATAT CTCTGAGTCA 
GATGGGTCTG AAGATGGTAA AGAAGAGCAA GAAGATAACC ATCCACTTCT GGGAGATCGC 
AAAAGATGGG CATCAAGGGC ATCAGATTTG GTAGTTCCTT TAGCTCAGTT ATCAATGGAA 
GATTCTCTCT CTGGAACAGA CCGGCAAACT ATGGGGGAAG TAATCAGGCT AGATATTGGG 
GAACCCCGCA GGAAAGTCTG GGGCAAAAGC CCTACTGATT CGGTCCTGAA GATTCTAGGA 
GAAGCGGAGT TGCAGCTACC AACTGAATTA CTGGAAGAAA TAGATGTAAT AAATGAGCCT 
GATGATTTGG AAGCAGTGAT GTTGAAAGAA ACAGAAGAAG ATAAAAAGCA CCAGAGCAAG 
GAATTTCCCA TTGCTGTTAA TGAAGTGTGG GTGAAAGAAA AACAGGATGA GGCACAACAG 
GATGCAAGAA ATGAAGCAGC TTTTGAGAAA GAAGTAGTGC TTGATGAAGT GCAATCACAA 
AATGAAGCTC CAGAAGAAAC TTGTTCCAGT GACTCTCTGC AACCTGAACC GTTATTCCAG 
AGAGTAATAC AGCCCCCAGC TGTACCTACA GCCTCACCAG TTCTATCATC TCAGTCTTCC 
CAAGAAGAGC CTTTTGTACC TCGTTCACGT TCCATATCGC CAGCAAAAAA TAAAGGGAAA 
AGTTCATTGT TTTTTGGACT TTCTACAGGT CTGTTTGATG CAAACAACCC AAAGATGTTG 
AGAACGTGTT CACTCCCGGA TCTTTACAAA CTGTACAGAA CCTTAGTTGA TGTTCCCAGC 
ATAGCAGATG TACAGCATCA ACACAGTCTT GAAATAGATG ATACGGAAGA TGAGCAAGCC 
AAAGAAGGAC CCTCTGATTC TGAGGATATC ATGTTTGGGG ACGCCGATAC AGATTTGCAG 
GAACTCCGGG CCTCAATGGA ACAATTACTT AGGGAGCAGC CTAGTGAGGA ATTAAGTGAA 
GAGGAGGAAT CTACTTTAAA GGCTAACGTC ATCAAATGCA TGACAAATGG TACAGAGCTG 
GATGAAGCAG ATGAAAATAA CCCCAGCAGT GAAAGTGCAC TTAATGAAGA ATGGCAGTCA 
GATAATAGTG ATGGAGATAT TGCCAGTGAA TGTGAAGAAT GTGATAGTGT CTTCAGCCAT 
TTGGAAGAGC TGAGATACAA CCTGGAACAG GAAATAGGCT TTGAGAAATT AATTGAAGTT 
TATAATAAAA TAAAGGCCAT ACATGAAGAT GAAGATGAAA ATATTGATAT TTGTTCAAGC 
ATAGTTCAGA CTATTCTGGG AAATGAACAC AAACACTTAT ATGCCAAAGT ACTTCACCTG 
GTCATGGCGG ATGGAGCCTA CCAGGAAGAT AATGACGAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_015285378.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015140864.1 Gallus gallus NIMA related kinase 1 (NEK1), transcript variant X5, mRNA.

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CloneID	OGa34987
Clone ID Related Accession (Same CDS sequence)	XM_015285378.1 , XM_015285378.2
Accession Version	XM_015285378.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3642bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	serine/threonine-protein kinase Nek1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015285378.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAAAT ACATTAAAGT GCAAAAAATT GGAGAAGGAT CCTTTGGGAA AGCATTTCTT 
GTTAAAGATA AAGAAAATAG CCAGCAGTAT GTTATTAAAG AAATTAACAT ATCTAAGATG 
TCAAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TACTGGCCAA CATGAAACAT 
CCAAATATTG TCCTGTATAG GGAGTCGTTT GAAGAAAATG GTTGTCTCTA CATAGTGATG 
GATTACTGTG AAGGAGGAGA TCTGTTCAAA AAAATAAATG CCCAGAAAGG AGTCCTGTTC 
TCTGAGGATC AGATTCTTGA TTGGTTTGTA CAGATCTGTT TGGCACTGAA ACATATACAT 
GATAGAAAAA TCTTACATCG CGACATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA 
ACAATACAGC TGGGAGATTT TGGAATCGCG AGAGTTCTGA ACAGTACTGT GGAATTGGCT 
CGCACTTGCA TAGGAACACC ATACTATTTA TCACCTGAAA TATGCCAGAA CAGGCCTTAC 
AACAATAAAA GTGATATCTG GGCCCTTGGT TGCGTTCTCT ATGAAATGTG CACACTTAAG 
CATGCATTTG AAGCTGGTAA CATGAAGAAC TTGGTACTGA AGATAATCTC TGGATCTTTC 
CCTCCTGTTT CTGTGCATTA TTCCTATGAC CTACGTAATC TACTGTCACA GTTATTTAAA 
AGGAATCCTA GGGATAGACC ATCAGTGAAC TCCATATTGG AGAAAAATTT CATAGCCAAA 
CGGGTTGAAA AATTTCTCAC ACCGCAGCTT ATTGCAGAAG AATTCAGTCA CAAAGTGTTT 
CACAAGTTTG GACCACATGC TGGACCAGCT AAAAGACCAG CTCAAGGACA TATCTCGGCT 
TCAATTGCAC CAGTGCAGAA AATTACCAAA CCTGCTGCAA AATACGGAGT GCCTTTAGCG 
ATGAAGAAGT GTTATGACGC ACCCAAAAAG CTTCATGAGA AAAAGTCATT GATTAAACTT 
AGGCAGGAAC CTTCAAAAAA GAAAAGGTTA GAATTGCCTG AAAAAGAAAA ACGCCAGAGA 
GATCAGATCA GTTTACTGAA GGCAGAGGAA ATGAGAAGAT TGGAAAAAGA AAGGATGGAA 
CGAATAAACA GAGCCAGGGA GCAAGGATGG AGGAATGTGC TTGGTTCAGG TGGAAGTGGT 
GATGTTAAGG CACCATATTA TGGCGGTGGA GGTGGTGTTG GTCCTTTTCC TGCCTCTTTC 
AGAGGACAGT ATGAGCACTA TCATGCTATT TTTGACCAGA TGCAAAAGGA AACCTTCAAA 
GTAACTAAAG AGAGGCAAGC CAAATGGAGG GCGGAGGTAC AAGGCCAAGA AGCTGTTGAG 
AGAGGAATTC CACCTGGAGT ACGTCCAGGA GTTCCTAAGG GGCCTGCAGG TCATCACCAT 
TTACCTGATG CTGATGCAAT TAGGAAAAAA GTGAAAAGGT TGAAGGAGGT GTCTAAACAA 
GCCAGTGCAA ACAGGCAGAA AGGATGGCTA CCTGCAGAAA GAGCAAAGCA AGTTGAAGAA 
TTCTGGCAAC GAAAGAGGGA AGCCATGCAA AACAAAGCTC GTGCTGAGGG ACACATGGGT 
ACACTGCAAA ACGTGGCAGA TATCTATGGA GGCAGGCCCA TGTCTGCAAG AGGAAGGAAA 
TCCAGAAACA AGGAAGAAGA GGAATATTTG GCAAGATTAA GACAGATACG ACTACAAAAC 
TTCAATGAGC GTCAACAGAT TAAAGCAAAA CTTCGTGGAG AGAAGAATGA AGCTCCCAGT 
TCTGATGGAC AAGAGTCAAG TGAGGAAGCA GAAATGAAAC GCAAAAAGAT AGAAGCACAG 
AAGGCCCAAG CAAATGCTCG AGCTGCAGTT CTGAAAGAAC AGTTAGAGCG AAAGAGGAAG 
GAGGCATATG AAAGAGAGAA AAGAGCTTGG GAAGAACATC TGATAGCAAA AGGAGTAAAG 
AATCCTAATG TGCCTTTTCA TGTGGGAGCA GCAGGACACA CTCCTGTGCA TGGAATACAA 
GAGCCTGGAG TGTCTCCTCC TAAGCAACAG CTTCCAGTTA AGCCCATCTC CATGACTTCT 
GCTTTGAAAG AAGTTGGCGT GGATGGAAAT GTAACCGCTG CCCAGGAGGT TAAAGAGGAA 
ATAAAAAAGC CAGATCTTAC AATGCAGAAC AAGAGGCAGA TACTGAAAAG ATTGAATCAA 
AATCTTAAAG CTCAGGAAGA TGAGAAAGGA AGAAAGGACG ATAAAAATAT CTCTGAGTCA 
GATGGGTCTG AAGATGGTAA AGAAGAGCAA GAAGATAACC ATCCACTTCT GGGAGATCGC 
AAAAGATGGG CATCAAGGGC ATCAGATTTG GTAGTTCCTT TAGCTCAGTT ATCAATGGAA 
GATTCTCTCT CTGGAACAGA CCGGCAAACT ATGGGGGAAG TAATCAGGCT AGATATTGGG 
GAACCCCGCA GGAAAGTCTG GGGCAAAAGC CCTACTGATT CGGTCCTGAA GATTCTAGGA 
GAAGCGGAGT TGCAGCTACC AACTGAATTA CTGGAAGAAA TAGATGTAAT AAATGAGCCT 
GATGATTTGG AAGCAGTGAT GTTGAAAGAA ACAGAAGAAG ATAAAAAGCA CCAGAGCAAG 
GAATTTCCCA TTGCTGTTAA TGAAGTGTGG GTGAAAGAAA AACAGGATGA GGCACAACAG 
GATGCAAGAA ATGAAGCAGC TTTTGAGAAA GAAGTAGTGC TTGATGAAGT GCAATCACAA 
AATGAAGCTC CAGAAGAAAC TTGTTCCAGT GACTCTCTGC AACCTGAACC GTTATTCCAG 
AGAGTAATAC AGCCCCCAGC TGTACCTACA GCCTCACCAG TTCTATCATC TCAGTCTTCC 
CAAGAAGAGC CTTTTGTACC TCGTTCACGT TCCATATCGC CAGCAAAAAA TAAAGGGAAA 
AGTTCATTGT TTTTTGGACT TTCTACAGGT CTGTTTGATG CAAACAACCC AAAGATGTTG 
AGAACGTGTT CACTCCCGGA TCTTTACAAA CTGTACAGAA CCTTAGTTGA TGTTCCCAGC 
ATAGCAGATG TACAGCATCA ACACAGTCTT GAAATAGATG ATACGGAAGA TGAGCAAGCC 
AAAGAAGGAC CCTCTGATTC TGAGGATATC ATGTTTGGGG ACGCCGATAC AGATTTGCAG 
GAACTCCGGG CCTCAATGGA ACAATTACTT AGGGAGCAGC CTAGTGAGGA ATTAAGTGAA 
GAGGAGGAAT CTACTTTAAA GGCTAACGTC ATCAAATGCA TGACAAATGG TACAGAGCTG 
GATGAAGCAG ATGAAAATAA CCCCAGCAGT GAAAGTGCAC TTAATGAAGA ATGGCAGTCA 
GATAATAGTG ATGGAGATAT TGCCAGTGAA TGTGAAGAAT GTGATAGTGT CTTCAGCCAT 
TTGGAAGAGC TGAGATACAA CCTGGAACAG GAAATAGGCT TTGAGAAATT AATTGAAGTT 
TATAATAAAA TAAAGGCCAT ACATGAAGAT GAAGATGAAA ATATTGATAT TTGTTCAAGC 
ATAGTTCAGA CTATTCTGGG AAATGAACAC AAACACTTAT ATGCCAAAGT ACTTCACCTG 
GTCATGGCGG ATGGAGCCTA CCAGGAAGAT AATGACGAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015140864.1
CDS	1004..4645
Translation	MDKYIKVQKIGEGSFGKAFLVKDKENSQQYVIKEINISKMSNKEREESRREVAVLANMKHPNIVLYRESFEENGCLYIVMDYCEGGDLFKKINAQKGVLFSEDQILDWFVQICLALKHIHDRKILHRDIKSQNIFLTKDGTIQLGDFGIARVLNSTVELARTCIGTPYYLSPEICQNRPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIISGSFPPVSVHYSYDLRNLLSQLFKRNPRDRPSVNSILEKNFIAKRVEKFLTPQLIAEEFSHKVFHKFGPHAGPAKRPAQGHISASIAPVQKITKPAAKYGVPLAMKKCYDAPKKLHEKKSLIKLRQEPSKKKRLELPEKEKRQRDQISLLKAEEMRRLEKERMERINRAREQGWRNVLGSGGSGDVKAPYYGGGGGVGPFPASFRGQYEHYHAIFDQMQKETFKVTKERQAKWRAEVQGQEAVERGIPPGVRPGVPKGPAGHHHLPDADAIRKKVKRLKEVSKQASANRQKGWLPAERAKQVEEFWQRKREAMQNKARAEGHMGTLQNVADIYGGRPMSARGRKSRNKEEEEYLARLRQIRLQNFNERQQIKAKLRGEKNEAPSSDGQESSEEAEMKRKKIEAQKAQANARAAVLKEQLERKRKEAYEREKRAWEEHLIAKGVKNPNVPFHVGAAGHTPVHGIQEPGVSPPKQQLPVKPISMTSALKEVGVDGNVTAAQEVKEEIKKPDLTMQNKRQILKRLNQNLKAQEDEKGRKDDKNISESDGSEDGKEEQEDNHPLLGDRKRWASRASDLVVPLAQLSMEDSLSGTDRQTMGEVIRLDIGEPRRKVWGKSPTDSVLKILGEAELQLPTELLEEIDVINEPDDLEAVMLKETEEDKKHQSKEFPIAVNEVWVKEKQDEAQQDARNEAAFEKEVVLDEVQSQNEAPEETCSSDSLQPEPLFQRVIQPPAVPTASPVLSSQSSQEEPFVPRSRSISPAKNKGKSSLFFGLSTGLFDANNPKMLRTCSLPDLYKLYRTLVDVPSIADVQHQHSLEIDDTEDEQAKEGPSDSEDIMFGDADTDLQELRASMEQLLREQPSEELSEEEESTLKANVIKCMTNGTELDEADENNPSSESALNEEWQSDNSDGDIASECEECDSVFSHLEELRYNLEQEIGFEKLIEVYNKIKAIHEDEDENIDICSSIVQTILGNEHKHLYAKVLHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_015285378.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus NIMA related kinase 1 (NEK1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015285378.2

ORF Insert Sequence:

ATGGATAAAT ACATTAAAGT GCAAAAAATT GGAGAAGGAT CCTTTGGGAA AGCATTTCTT 
GTTAAAGATA AAGAAAATAG CCAGCAGTAT GTTATTAAAG AAATTAACAT ATCTAAGATG 
TCAAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TACTGGCCAA CATGAAACAT 
CCAAATATTG TCCTGTATAG GGAGTCGTTT GAAGAAAATG GTTGTCTCTA CATAGTGATG 
GATTACTGTG AAGGAGGAGA TCTGTTCAAA AAAATAAATG CCCAGAAAGG AGTCCTGTTC 
TCTGAGGATC AGATTCTTGA TTGGTTTGTA CAGATCTGTT TGGCACTGAA ACATATACAT 
GATAGAAAAA TCTTACATCG CGACATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA 
ACAATACAGC TGGGAGATTT TGGAATCGCG AGAGTTCTGA ACAGTACTGT GGAATTGGCT 
CGCACTTGCA TAGGAACACC ATACTATTTA TCACCTGAAA TATGCCAGAA CAGGCCTTAC 
AACAATAAAA GTGATATCTG GGCCCTTGGT TGCGTTCTCT ATGAAATGTG CACACTTAAG 
CATGCATTTG AAGCTGGTAA CATGAAGAAC TTGGTACTGA AGATAATCTC TGGATCTTTC 
CCTCCTGTTT CTGTGCATTA TTCCTATGAC CTACGTAATC TACTGTCACA GTTATTTAAA 
AGGAATCCTA GGGATAGACC ATCAGTGAAC TCCATATTGG AGAAAAATTT CATAGCCAAA 
CGGGTTGAAA AATTTCTCAC ACCGCAGCTT ATTGCAGAAG AATTCAGTCA CAAAGTGTTT 
CACAAGTTTG GACCACATGC TGGACCAGCT AAAAGACCAG CTCAAGGACA TATCTCGGCT 
TCAATTGCAC CAGTGCAGAA AATTACCAAA CCTGCTGCAA AATACGGAGT GCCTTTAGCG 
ATGAAGAAGT GTTATGACGC ACCCAAAAAG CTTCATGAGA AAAAGTCATT GATTAAACTT 
AGGCAGGAAC CTTCAAAAAA GAAAAGGTTA GAATTGCCTG AAAAAGAAAA ACGCCAGAGA 
GATCAGATCA GTTTACTGAA GGCAGAGGAA ATGAGAAGAT TGGAAAAAGA AAGGATGGAA 
CGAATAAACA GAGCCAGGGA GCAAGGATGG AGGAATGTGC TTGGTTCAGG TGGAAGTGGT 
GATGTTAAGG CACCATATTA TGGCGGTGGA GGTGGTGTTG GTCCTTTTCC TGCCTCTTTC 
AGAGGACAGT ATGAGCACTA TCATGCTATT TTTGACCAGA TGCAAAAGGA AACCTTCAAA 
GTAACTAAAG AGAGGCAAGC CAAATGGAGG GCGGAGGTAC AAGGCCAAGA AGCTGTTGAG 
AGAGGAATTC CACCTGGAGT ACGTCCAGGA GTTCCTAAGG GGCCTGCAGG TCATCACCAT 
TTACCTGATG CTGATGCAAT TAGGAAAAAA GTGAAAAGGT TGAAGGAGGT GTCTAAACAA 
GCCAGTGCAA ACAGGCAGAA AGGATGGCTA CCTGCAGAAA GAGCAAAGCA AGTTGAAGAA 
TTCTGGCAAC GAAAGAGGGA AGCCATGCAA AACAAAGCTC GTGCTGAGGG ACACATGGGT 
ACACTGCAAA ACGTGGCAGA TATCTATGGA GGCAGGCCCA TGTCTGCAAG AGGAAGGAAA 
TCCAGAAACA AGGAAGAAGA GGAATATTTG GCAAGATTAA GACAGATACG ACTACAAAAC 
TTCAATGAGC GTCAACAGAT TAAAGCAAAA CTTCGTGGAG AGAAGAATGA AGCTCCCAGT 
TCTGATGGAC AAGAGTCAAG TGAGGAAGCA GAAATGAAAC GCAAAAAGAT AGAAGCACAG 
AAGGCCCAAG CAAATGCTCG AGCTGCAGTT CTGAAAGAAC AGTTAGAGCG AAAGAGGAAG 
GAGGCATATG AAAGAGAGAA AAGAGCTTGG GAAGAACATC TGATAGCAAA AGGAGTAAAG 
AATCCTAATG TGCCTTTTCA TGTGGGAGCA GCAGGACACA CTCCTGTGCA TGGAATACAA 
GAGCCTGGAG TGTCTCCTCC TAAGCAACAG CTTCCAGTTA AGCCCATCTC CATGACTTCT 
GCTTTGAAAG AAGTTGGCGT GGATGGAAAT GTAACCGCTG CCCAGGAGGT TAAAGAGGAA 
ATAAAAAAGC CAGATCTTAC AATGCAGAAC AAGAGGCAGA TACTGAAAAG ATTGAATCAA 
AATCTTAAAG CTCAGGAAGA TGAGAAAGGA AGAAAGGACG ATAAAAATAT CTCTGAGTCA 
GATGGGTCTG AAGATGGTAA AGAAGAGCAA GAAGATAACC ATCCACTTCT GGGAGATCGC 
AAAAGATGGG CATCAAGGGC ATCAGATTTG GTAGTTCCTT TAGCTCAGTT ATCAATGGAA 
GATTCTCTCT CTGGAACAGA CCGGCAAACT ATGGGGGAAG TAATCAGGCT AGATATTGGG 
GAACCCCGCA GGAAAGTCTG GGGCAAAAGC CCTACTGATT CGGTCCTGAA GATTCTAGGA 
GAAGCGGAGT TGCAGCTACC AACTGAATTA CTGGAAGAAA TAGATGTAAT AAATGAGCCT 
GATGATTTGG AAGCAGTGAT GTTGAAAGAA ACAGAAGAAG ATAAAAAGCA CCAGAGCAAG 
GAATTTCCCA TTGCTGTTAA TGAAGTGTGG GTGAAAGAAA AACAGGATGA GGCACAACAG 
GATGCAAGAA ATGAAGCAGC TTTTGAGAAA GAAGTAGTGC TTGATGAAGT GCAATCACAA 
AATGAAGCTC CAGAAGAAAC TTGTTCCAGT GACTCTCTGC AACCTGAACC GTTATTCCAG 
AGAGTAATAC AGCCCCCAGC TGTACCTACA GCCTCACCAG TTCTATCATC TCAGTCTTCC 
CAAGAAGAGC CTTTTGTACC TCGTTCACGT TCCATATCGC CAGCAAAAAA TAAAGGGAAA 
AGTTCATTGT TTTTTGGACT TTCTACAGGT CTGTTTGATG CAAACAACCC AAAGATGTTG 
AGAACGTGTT CACTCCCGGA TCTTTACAAA CTGTACAGAA CCTTAGTTGA TGTTCCCAGC 
ATAGCAGATG TACAGCATCA ACACAGTCTT GAAATAGATG ATACGGAAGA TGAGCAAGCC 
AAAGAAGGAC CCTCTGATTC TGAGGATATC ATGTTTGGGG ACGCCGATAC AGATTTGCAG 
GAACTCCGGG CCTCAATGGA ACAATTACTT AGGGAGCAGC CTAGTGAGGA ATTAAGTGAA 
GAGGAGGAAT CTACTTTAAA GGCTAACGTC ATCAAATGCA TGACAAATGG TACAGAGCTG 
GATGAAGCAG ATGAAAATAA CCCCAGCAGT GAAAGTGCAC TTAATGAAGA ATGGCAGTCA 
GATAATAGTG ATGGAGATAT TGCCAGTGAA TGTGAAGAAT GTGATAGTGT CTTCAGCCAT 
TTGGAAGAGC TGAGATACAA CCTGGAACAG GAAATAGGCT TTGAGAAATT AATTGAAGTT 
TATAATAAAA TAAAGGCCAT ACATGAAGAT GAAGATGAAA ATATTGATAT TTGTTCAAGC 
ATAGTTCAGA CTATTCTGGG AAATGAACAC AAACACTTAT ATGCCAAAGT ACTTCACCTG 
GTCATGGCGG ATGGAGCCTA CCAGGAAGAT AATGACGAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_015285380.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015140866.1 Gallus gallus NIMA-related kinase 1 (NEK1), transcript variant X7, mRNA.

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CloneID	OGa34988
Clone ID Related Accession (Same CDS sequence)	XM_015285380.1 , XM_015285380.2
Accession Version	XM_015285380.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3582bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	serine/threonine-protein kinase Nek1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455855.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAAAT ACATTAAAGT GCAAAAAATT GGAGAAGGAT CCTTTGGGAA AGCATTTCTT 
GTTAAAGATA AAGAAAATAG CCAGCAGTAT GTTATTAAAG AAATTAACAT ATCTAAGATG 
TCAAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TACTGGCCAA CATGAAACAT 
CCAAATATTG TCCTGTATAG GGAGTCGTTT GAAGAAAATG GTTGTCTCTA CATAGTGATG 
GATTACTGTG AAGGAGGAGA TCTGTTCAAA AAAATAAATG CCCAGAAAGG AGTCCTGTTC 
TCTGAGGATC AGATTCTTGA TTGGTTTGTA CAGATCTGTT TGGCACTGAA ACATATACAT 
GATAGAAAAA TCTTACATCG CGACATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA 
ACAATACAGC TGGGAGATTT TGGAATCGCG AGAGTTCTGA ACAGTACTGT GGAATTGGCT 
CGCACTTGCA TAGGAACACC ATACTATTTA TCACCTGAAA TATGCCAGAA CAGGCCTTAC 
AACAATAAAA GTGATATCTG GGCCCTTGGT TGCGTTCTCT ATGAAATGTG CACACTTAAG 
CATGCATTTG AAGCTGGTAA CATGAAGAAC TTGGTACTGA AGATAATCTC TGGATCTTTC 
CCTCCTGTTT CTGTGCATTA TTCCTATGAC CTACGTAATC TACTGTCACA GTTATTTAAA 
AGGAATCCTA GGGATAGACC ATCAGTGAAC TCCATATTGG AGAAAAATTT CATAGCCAAA 
CGGGTTGAAA AATTTCTCAC ACCGCAGCTT ATTGCAGAAG AATTCAGTCA CAAAGTGTTT 
CACAAGTTTG GACCACATGC TGGACCAGCT AAAAGACCAG CTCAAGGACA TATCTCGGCT 
TCAATTGCAC CAGTGCAGAA AATTACCAAA CCTGCTGCAA AATACGGAGT GCCTTTAGCG 
ATGAAGAAGT GTTATGACGC ACCCAAAAAG CTTCATGAGA AAAAGTCATT GATTAAACTT 
AGGCAGATCA GTTTACTGAA GGCAGAGGAA ATGAGAAGAT TGGAAAAAGA AAGGATGGAA 
CGAATAAACA GAGCCAGGGA GCAAGGATGG AGGAATGTGC TTGGTTCAGG TGGAAGTGGT 
GATGTTAAGG CACCATATTA TGGCGGTGGA GGTGGTGTTG GTCCTTTTCC TGCCTCTTTC 
AGAGGACAGT ATGAGCACTA TCATGCTATT TTTGACCAGA TGCAAAAGGA AACCTTCAAA 
GTAACTAAAG AGAGGCAAGC CAAATGGAGG GCGGAGGTAC AAGGCCAAGA AGCTGTTGAG 
AGAGGAATTC CACCTGGAGT ACGTCCAGGA GTTCCTAAGG GGCCTGCAGG TCATCACCAT 
TTACCTGATG CTGATGCAAT TAGGAAAAAA GTGAAAAGGT TGAAGGAGGT GTCTAAACAA 
GCCAGTGCAA ACAGGCAGAA AGGATGGCTA CCTGCAGAAA GAGCAAAGCA AGTTGAAGAA 
TTCTGGCAAC GAAAGAGGGA AGCCATGCAA AACAAAGCTC GTGCTGAGGG ACACATGGGT 
ACACTGCAAA ACGTGGCAGA TATCTATGGA GGCAGGCCCA TGTCTGCAAG AGGAAGGAAA 
TCCAGAAACA AGGAAGAAGA GGAATATTTG GCAAGATTAA GACAGATACG ACTACAAAAC 
TTCAATGAGC GTCAACAGAT TAAAGCAAAA CTTCGTGGAG AGAAGAATGA AGCTCCCAGT 
TCTGATGGAC AAGAGTCAAG TGAGGAAGCA GAAATGAAAC GCAAAAAGAT AGAAGCACAG 
AAGGCCCAAG CAAATGCTCG AGCTGCAGTT CTGAAAGAAC AGTTAGAGCG AAAGAGGAAG 
GAGGCATATG AAAGAGAGAA AAGAGCTTGG GAAGAACATC TGATAGCAAA AGGAGTAAAG 
AATCCTAATG TGCCTTTTCA TGTGGGAGCA GCAGGACACA CTCCTGTGCA TGGAATACAA 
GAGCCTGGAG TGTCTCCTCC TAAGCAACAG CTTCCAGTTA AGCCCATCTC CATGACTTCT 
GCTTTGAAAG AAGTTGGCGT GGATGGAAAT GTAACCGCTG CCCAGGAGGT TAAAGAGGAA 
ATAAAAAAGC CAGATCTTAC AATGCAGAAC AAGAGGCAGA TACTGAAAAG ATTGAATCAA 
AATCTTAAAG CTCAGGAAGA TGAGAAAGGA AGAAAGGACG ATAAAAATAT CTCTGAGTCA 
GATGGGTCTG AAGATGGTAA AGAAGAGCAA GAAGATAACC ATCCACTTCT GGGAGATCGC 
AAAAGATGGG CATCAAGGGC ATCAGATTTG GTAGTTCCTT TAGCTCAGTT ATCAATGGAA 
GATTCTCTCT CTGGAACAGA CCGGCAAACT ATGGGGGAAG TAATCAGGCT AGATATTGGG 
GAACCCCGCA GGAAAGTCTG GGGCAAAAGC CCTACTGATT CGGTCCTGAA GATTCTAGGA 
GAAGCGGAGT TGCAGCTACC AACTGAATTA CTGGAAGAAA TAGATGTAAT AAATGAGCCT 
GATGATTTGG AAGCAGTGAT GTTGAAAGAA ACAGAAGAAG ATAAAAAGCA CCAGAGCAAG 
GAATTTCCCA TTGCTGTTAA TGAAGTGTGG GTGAAAGAAA AACAGGATGA GGCACAACAG 
GATGCAAGAA ATGAAGCAGC TTTTGAGAAA GAAGTAGTGC TTGATGAAGT GCAATCACAA 
AATGAAGCTC CAGAAGAAAC TTGTTCCAGT GACTCTCTGC AACCTGAACC GTTATTCCAG 
AGAGTAATAC AGCCCCCAGC TGTACCTACA GCCTCACCAG TTCTATCATC TCAGTCTTCC 
CAAGAAGAGC CTTTTGTACC TCGTTCACGT TCCATATCGC CAGCAAAAAA TAAAGGGAAA 
AGTTCATTGT TTTTTGGACT TTCTACAGGT CTGTTTGATG CAAACAACCC AAAGATGTTG 
AGAACGTGTT CACTCCCGGA TCTTTACAAA CTGTACAGAA CCTTAGTTGA TGTTCCCAGC 
ATAGCAGATG TACAGCATCA ACACAGTCTT GAAATAGATG ATACGGAAGA TGAGCAAGCC 
AAAGAAGGAC CCTCTGATTC TGAGGATATC ATGTTTGGGG ACGCCGATAC AGATTTGCAG 
GAACTCCGGG CCTCAATGGA ACAATTACTT AGGGAGCAGC CTAGTGAGGA ATTAAGTGAA 
GAGGAGGAAT CTACTTTAAA GGCTAACGTC ATCAAATGCA TGACAAATGG TACAGAGCTG 
GATGAAGCAG ATGAAAATAA CCCCAGCAGT GAAAGTGCAC TTAATGAAGA ATGGCAGTCA 
GATAATAGTG ATGGAGATAT TGCCAGTGAA TGTGAAGAAT GTGATAGTGT CTTCAGCCAT 
TTGGAAGAGC TGAGATACAA CCTGGAACAG GAAATAGGCT TTGAGAAATT AATTGAAGTT 
TATAATAAAA TAAAGGCCAT ACATGAAGAT GAAGATGAAA ATATTGATAT TTGTTCAAGC 
ATAGTTCAGA CTATTCTGGG AAATGAACAC AAACACTTAT ATGCCAAAGT ACTTCACCTG 
GTCATGGCGG ATGGAGCCTA CCAGGAAGAT AATGACGAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015140866.1
CDS	1006..4587
Translation	MDKYIKVQKIGEGSFGKAFLVKDKENSQQYVIKEINISKMSNKEREESRREVAVLANMKHPNIVLYRESFEENGCLYIVMDYCEGGDLFKKINAQKGVLFSEDQILDWFVQICLALKHIHDRKILHRDIKSQNIFLTKDGTIQLGDFGIARVLNSTVELARTCIGTPYYLSPEICQNRPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIISGSFPPVSVHYSYDLRNLLSQLFKRNPRDRPSVNSILEKNFIAKRVEKFLTPQLIAEEFSHKVFHKFGPHAGPAKRPAQGHISASIAPVQKITKPAAKYGVPLAMKKCYDAPKKLHEKKSLIKLRQISLLKAEEMRRLEKERMERINRAREQGWRNVLGSGGSGDVKAPYYGGGGGVGPFPASFRGQYEHYHAIFDQMQKETFKVTKERQAKWRAEVQGQEAVERGIPPGVRPGVPKGPAGHHHLPDADAIRKKVKRLKEVSKQASANRQKGWLPAERAKQVEEFWQRKREAMQNKARAEGHMGTLQNVADIYGGRPMSARGRKSRNKEEEEYLARLRQIRLQNFNERQQIKAKLRGEKNEAPSSDGQESSEEAEMKRKKIEAQKAQANARAAVLKEQLERKRKEAYEREKRAWEEHLIAKGVKNPNVPFHVGAAGHTPVHGIQEPGVSPPKQQLPVKPISMTSALKEVGVDGNVTAAQEVKEEIKKPDLTMQNKRQILKRLNQNLKAQEDEKGRKDDKNISESDGSEDGKEEQEDNHPLLGDRKRWASRASDLVVPLAQLSMEDSLSGTDRQTMGEVIRLDIGEPRRKVWGKSPTDSVLKILGEAELQLPTELLEEIDVINEPDDLEAVMLKETEEDKKHQSKEFPIAVNEVWVKEKQDEAQQDARNEAAFEKEVVLDEVQSQNEAPEETCSSDSLQPEPLFQRVIQPPAVPTASPVLSSQSSQEEPFVPRSRSISPAKNKGKSSLFFGLSTGLFDANNPKMLRTCSLPDLYKLYRTLVDVPSIADVQHQHSLEIDDTEDEQAKEGPSDSEDIMFGDADTDLQELRASMEQLLREQPSEELSEEEESTLKANVIKCMTNGTELDEADENNPSSESALNEEWQSDNSDGDIASECEECDSVFSHLEELRYNLEQEIGFEKLIEVYNKIKAIHEDEDENIDICSSIVQTILGNEHKHLYAKVLHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_015285380.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus NIMA-related kinase 1 (NEK1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015285380.1

ORF Insert Sequence:

ATGGATAAAT ACATTAAAGT GCAAAAAATT GGAGAAGGAT CCTTTGGGAA AGCATTTCTT 
GTTAAAGATA AAGAAAATAG CCAGCAGTAT GTTATTAAAG AAATTAACAT ATCTAAGATG 
TCAAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TACTGGCCAA CATGAAACAT 
CCAAATATTG TCCTGTATAG GGAGTCGTTT GAAGAAAATG GTTGTCTCTA CATAGTGATG 
GATTACTGTG AAGGAGGAGA TCTGTTCAAA AAAATAAATG CCCAGAAAGG AGTCCTGTTC 
TCTGAGGATC AGATTCTTGA TTGGTTTGTA CAGATCTGTT TGGCACTGAA ACATATACAT 
GATAGAAAAA TCTTACATCG CGACATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA 
ACAATACAGC TGGGAGATTT TGGAATCGCG AGAGTTCTGA ACAGTACTGT GGAATTGGCT 
CGCACTTGCA TAGGAACACC ATACTATTTA TCACCTGAAA TATGCCAGAA CAGGCCTTAC 
AACAATAAAA GTGATATCTG GGCCCTTGGT TGCGTTCTCT ATGAAATGTG CACACTTAAG 
CATGCATTTG AAGCTGGTAA CATGAAGAAC TTGGTACTGA AGATAATCTC TGGATCTTTC 
CCTCCTGTTT CTGTGCATTA TTCCTATGAC CTACGTAATC TACTGTCACA GTTATTTAAA 
AGGAATCCTA GGGATAGACC ATCAGTGAAC TCCATATTGG AGAAAAATTT CATAGCCAAA 
CGGGTTGAAA AATTTCTCAC ACCGCAGCTT ATTGCAGAAG AATTCAGTCA CAAAGTGTTT 
CACAAGTTTG GACCACATGC TGGACCAGCT AAAAGACCAG CTCAAGGACA TATCTCGGCT 
TCAATTGCAC CAGTGCAGAA AATTACCAAA CCTGCTGCAA AATACGGAGT GCCTTTAGCG 
ATGAAGAAGT GTTATGACGC ACCCAAAAAG CTTCATGAGA AAAAGTCATT GATTAAACTT 
AGGCAGATCA GTTTACTGAA GGCAGAGGAA ATGAGAAGAT TGGAAAAAGA AAGGATGGAA 
CGAATAAACA GAGCCAGGGA GCAAGGATGG AGGAATGTGC TTGGTTCAGG TGGAAGTGGT 
GATGTTAAGG CACCATATTA TGGCGGTGGA GGTGGTGTTG GTCCTTTTCC TGCCTCTTTC 
AGAGGACAGT ATGAGCACTA TCATGCTATT TTTGACCAGA TGCAAAAGGA AACCTTCAAA 
GTAACTAAAG AGAGGCAAGC CAAATGGAGG GCGGAGGTAC AAGGCCAAGA AGCTGTTGAG 
AGAGGAATTC CACCTGGAGT ACGTCCAGGA GTTCCTAAGG GGCCTGCAGG TCATCACCAT 
TTACCTGATG CTGATGCAAT TAGGAAAAAA GTGAAAAGGT TGAAGGAGGT GTCTAAACAA 
GCCAGTGCAA ACAGGCAGAA AGGATGGCTA CCTGCAGAAA GAGCAAAGCA AGTTGAAGAA 
TTCTGGCAAC GAAAGAGGGA AGCCATGCAA AACAAAGCTC GTGCTGAGGG ACACATGGGT 
ACACTGCAAA ACGTGGCAGA TATCTATGGA GGCAGGCCCA TGTCTGCAAG AGGAAGGAAA 
TCCAGAAACA AGGAAGAAGA GGAATATTTG GCAAGATTAA GACAGATACG ACTACAAAAC 
TTCAATGAGC GTCAACAGAT TAAAGCAAAA CTTCGTGGAG AGAAGAATGA AGCTCCCAGT 
TCTGATGGAC AAGAGTCAAG TGAGGAAGCA GAAATGAAAC GCAAAAAGAT AGAAGCACAG 
AAGGCCCAAG CAAATGCTCG AGCTGCAGTT CTGAAAGAAC AGTTAGAGCG AAAGAGGAAG 
GAGGCATATG AAAGAGAGAA AAGAGCTTGG GAAGAACATC TGATAGCAAA AGGAGTAAAG 
AATCCTAATG TGCCTTTTCA TGTGGGAGCA GCAGGACACA CTCCTGTGCA TGGAATACAA 
GAGCCTGGAG TGTCTCCTCC TAAGCAACAG CTTCCAGTTA AGCCCATCTC CATGACTTCT 
GCTTTGAAAG AAGTTGGCGT GGATGGAAAT GTAACCGCTG CCCAGGAGGT TAAAGAGGAA 
ATAAAAAAGC CAGATCTTAC AATGCAGAAC AAGAGGCAGA TACTGAAAAG ATTGAATCAA 
AATCTTAAAG CTCAGGAAGA TGAGAAAGGA AGAAAGGACG ATAAAAATAT CTCTGAGTCA 
GATGGGTCTG AAGATGGTAA AGAAGAGCAA GAAGATAACC ATCCACTTCT GGGAGATCGC 
AAAAGATGGG CATCAAGGGC ATCAGATTTG GTAGTTCCTT TAGCTCAGTT ATCAATGGAA 
GATTCTCTCT CTGGAACAGA CCGGCAAACT ATGGGGGAAG TAATCAGGCT AGATATTGGG 
GAACCCCGCA GGAAAGTCTG GGGCAAAAGC CCTACTGATT CGGTCCTGAA GATTCTAGGA 
GAAGCGGAGT TGCAGCTACC AACTGAATTA CTGGAAGAAA TAGATGTAAT AAATGAGCCT 
GATGATTTGG AAGCAGTGAT GTTGAAAGAA ACAGAAGAAG ATAAAAAGCA CCAGAGCAAG 
GAATTTCCCA TTGCTGTTAA TGAAGTGTGG GTGAAAGAAA AACAGGATGA GGCACAACAG 
GATGCAAGAA ATGAAGCAGC TTTTGAGAAA GAAGTAGTGC TTGATGAAGT GCAATCACAA 
AATGAAGCTC CAGAAGAAAC TTGTTCCAGT GACTCTCTGC AACCTGAACC GTTATTCCAG 
AGAGTAATAC AGCCCCCAGC TGTACCTACA GCCTCACCAG TTCTATCATC TCAGTCTTCC 
CAAGAAGAGC CTTTTGTACC TCGTTCACGT TCCATATCGC CAGCAAAAAA TAAAGGGAAA 
AGTTCATTGT TTTTTGGACT TTCTACAGGT CTGTTTGATG CAAACAACCC AAAGATGTTG 
AGAACGTGTT CACTCCCGGA TCTTTACAAA CTGTACAGAA CCTTAGTTGA TGTTCCCAGC 
ATAGCAGATG TACAGCATCA ACACAGTCTT GAAATAGATG ATACGGAAGA TGAGCAAGCC 
AAAGAAGGAC CCTCTGATTC TGAGGATATC ATGTTTGGGG ACGCCGATAC AGATTTGCAG 
GAACTCCGGG CCTCAATGGA ACAATTACTT AGGGAGCAGC CTAGTGAGGA ATTAAGTGAA 
GAGGAGGAAT CTACTTTAAA GGCTAACGTC ATCAAATGCA TGACAAATGG TACAGAGCTG 
GATGAAGCAG ATGAAAATAA CCCCAGCAGT GAAAGTGCAC TTAATGAAGA ATGGCAGTCA 
GATAATAGTG ATGGAGATAT TGCCAGTGAA TGTGAAGAAT GTGATAGTGT CTTCAGCCAT 
TTGGAAGAGC TGAGATACAA CCTGGAACAG GAAATAGGCT TTGAGAAATT AATTGAAGTT 
TATAATAAAA TAAAGGCCAT ACATGAAGAT GAAGATGAAA ATATTGATAT TTGTTCAAGC 
ATAGTTCAGA CTATTCTGGG AAATGAACAC AAACACTTAT ATGCCAAAGT ACTTCACCTG 
GTCATGGCGG ATGGAGCCTA CCAGGAAGAT AATGACGAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_015285380.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015140866.1 Gallus gallus NIMA related kinase 1 (NEK1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OGa34988
Clone ID Related Accession (Same CDS sequence)	XM_015285380.1 , XM_015285380.2
Accession Version	XM_015285380.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3582bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	serine/threonine-protein kinase Nek1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015285380.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAAAT ACATTAAAGT GCAAAAAATT GGAGAAGGAT CCTTTGGGAA AGCATTTCTT 
GTTAAAGATA AAGAAAATAG CCAGCAGTAT GTTATTAAAG AAATTAACAT ATCTAAGATG 
TCAAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TACTGGCCAA CATGAAACAT 
CCAAATATTG TCCTGTATAG GGAGTCGTTT GAAGAAAATG GTTGTCTCTA CATAGTGATG 
GATTACTGTG AAGGAGGAGA TCTGTTCAAA AAAATAAATG CCCAGAAAGG AGTCCTGTTC 
TCTGAGGATC AGATTCTTGA TTGGTTTGTA CAGATCTGTT TGGCACTGAA ACATATACAT 
GATAGAAAAA TCTTACATCG CGACATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA 
ACAATACAGC TGGGAGATTT TGGAATCGCG AGAGTTCTGA ACAGTACTGT GGAATTGGCT 
CGCACTTGCA TAGGAACACC ATACTATTTA TCACCTGAAA TATGCCAGAA CAGGCCTTAC 
AACAATAAAA GTGATATCTG GGCCCTTGGT TGCGTTCTCT ATGAAATGTG CACACTTAAG 
CATGCATTTG AAGCTGGTAA CATGAAGAAC TTGGTACTGA AGATAATCTC TGGATCTTTC 
CCTCCTGTTT CTGTGCATTA TTCCTATGAC CTACGTAATC TACTGTCACA GTTATTTAAA 
AGGAATCCTA GGGATAGACC ATCAGTGAAC TCCATATTGG AGAAAAATTT CATAGCCAAA 
CGGGTTGAAA AATTTCTCAC ACCGCAGCTT ATTGCAGAAG AATTCAGTCA CAAAGTGTTT 
CACAAGTTTG GACCACATGC TGGACCAGCT AAAAGACCAG CTCAAGGACA TATCTCGGCT 
TCAATTGCAC CAGTGCAGAA AATTACCAAA CCTGCTGCAA AATACGGAGT GCCTTTAGCG 
ATGAAGAAGT GTTATGACGC ACCCAAAAAG CTTCATGAGA AAAAGTCATT GATTAAACTT 
AGGCAGATCA GTTTACTGAA GGCAGAGGAA ATGAGAAGAT TGGAAAAAGA AAGGATGGAA 
CGAATAAACA GAGCCAGGGA GCAAGGATGG AGGAATGTGC TTGGTTCAGG TGGAAGTGGT 
GATGTTAAGG CACCATATTA TGGCGGTGGA GGTGGTGTTG GTCCTTTTCC TGCCTCTTTC 
AGAGGACAGT ATGAGCACTA TCATGCTATT TTTGACCAGA TGCAAAAGGA AACCTTCAAA 
GTAACTAAAG AGAGGCAAGC CAAATGGAGG GCGGAGGTAC AAGGCCAAGA AGCTGTTGAG 
AGAGGAATTC CACCTGGAGT ACGTCCAGGA GTTCCTAAGG GGCCTGCAGG TCATCACCAT 
TTACCTGATG CTGATGCAAT TAGGAAAAAA GTGAAAAGGT TGAAGGAGGT GTCTAAACAA 
GCCAGTGCAA ACAGGCAGAA AGGATGGCTA CCTGCAGAAA GAGCAAAGCA AGTTGAAGAA 
TTCTGGCAAC GAAAGAGGGA AGCCATGCAA AACAAAGCTC GTGCTGAGGG ACACATGGGT 
ACACTGCAAA ACGTGGCAGA TATCTATGGA GGCAGGCCCA TGTCTGCAAG AGGAAGGAAA 
TCCAGAAACA AGGAAGAAGA GGAATATTTG GCAAGATTAA GACAGATACG ACTACAAAAC 
TTCAATGAGC GTCAACAGAT TAAAGCAAAA CTTCGTGGAG AGAAGAATGA AGCTCCCAGT 
TCTGATGGAC AAGAGTCAAG TGAGGAAGCA GAAATGAAAC GCAAAAAGAT AGAAGCACAG 
AAGGCCCAAG CAAATGCTCG AGCTGCAGTT CTGAAAGAAC AGTTAGAGCG AAAGAGGAAG 
GAGGCATATG AAAGAGAGAA AAGAGCTTGG GAAGAACATC TGATAGCAAA AGGAGTAAAG 
AATCCTAATG TGCCTTTTCA TGTGGGAGCA GCAGGACACA CTCCTGTGCA TGGAATACAA 
GAGCCTGGAG TGTCTCCTCC TAAGCAACAG CTTCCAGTTA AGCCCATCTC CATGACTTCT 
GCTTTGAAAG AAGTTGGCGT GGATGGAAAT GTAACCGCTG CCCAGGAGGT TAAAGAGGAA 
ATAAAAAAGC CAGATCTTAC AATGCAGAAC AAGAGGCAGA TACTGAAAAG ATTGAATCAA 
AATCTTAAAG CTCAGGAAGA TGAGAAAGGA AGAAAGGACG ATAAAAATAT CTCTGAGTCA 
GATGGGTCTG AAGATGGTAA AGAAGAGCAA GAAGATAACC ATCCACTTCT GGGAGATCGC 
AAAAGATGGG CATCAAGGGC ATCAGATTTG GTAGTTCCTT TAGCTCAGTT ATCAATGGAA 
GATTCTCTCT CTGGAACAGA CCGGCAAACT ATGGGGGAAG TAATCAGGCT AGATATTGGG 
GAACCCCGCA GGAAAGTCTG GGGCAAAAGC CCTACTGATT CGGTCCTGAA GATTCTAGGA 
GAAGCGGAGT TGCAGCTACC AACTGAATTA CTGGAAGAAA TAGATGTAAT AAATGAGCCT 
GATGATTTGG AAGCAGTGAT GTTGAAAGAA ACAGAAGAAG ATAAAAAGCA CCAGAGCAAG 
GAATTTCCCA TTGCTGTTAA TGAAGTGTGG GTGAAAGAAA AACAGGATGA GGCACAACAG 
GATGCAAGAA ATGAAGCAGC TTTTGAGAAA GAAGTAGTGC TTGATGAAGT GCAATCACAA 
AATGAAGCTC CAGAAGAAAC TTGTTCCAGT GACTCTCTGC AACCTGAACC GTTATTCCAG 
AGAGTAATAC AGCCCCCAGC TGTACCTACA GCCTCACCAG TTCTATCATC TCAGTCTTCC 
CAAGAAGAGC CTTTTGTACC TCGTTCACGT TCCATATCGC CAGCAAAAAA TAAAGGGAAA 
AGTTCATTGT TTTTTGGACT TTCTACAGGT CTGTTTGATG CAAACAACCC AAAGATGTTG 
AGAACGTGTT CACTCCCGGA TCTTTACAAA CTGTACAGAA CCTTAGTTGA TGTTCCCAGC 
ATAGCAGATG TACAGCATCA ACACAGTCTT GAAATAGATG ATACGGAAGA TGAGCAAGCC 
AAAGAAGGAC CCTCTGATTC TGAGGATATC ATGTTTGGGG ACGCCGATAC AGATTTGCAG 
GAACTCCGGG CCTCAATGGA ACAATTACTT AGGGAGCAGC CTAGTGAGGA ATTAAGTGAA 
GAGGAGGAAT CTACTTTAAA GGCTAACGTC ATCAAATGCA TGACAAATGG TACAGAGCTG 
GATGAAGCAG ATGAAAATAA CCCCAGCAGT GAAAGTGCAC TTAATGAAGA ATGGCAGTCA 
GATAATAGTG ATGGAGATAT TGCCAGTGAA TGTGAAGAAT GTGATAGTGT CTTCAGCCAT 
TTGGAAGAGC TGAGATACAA CCTGGAACAG GAAATAGGCT TTGAGAAATT AATTGAAGTT 
TATAATAAAA TAAAGGCCAT ACATGAAGAT GAAGATGAAA ATATTGATAT TTGTTCAAGC 
ATAGTTCAGA CTATTCTGGG AAATGAACAC AAACACTTAT ATGCCAAAGT ACTTCACCTG 
GTCATGGCGG ATGGAGCCTA CCAGGAAGAT AATGACGAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015140866.1
CDS	1004..4585
Translation	MDKYIKVQKIGEGSFGKAFLVKDKENSQQYVIKEINISKMSNKEREESRREVAVLANMKHPNIVLYRESFEENGCLYIVMDYCEGGDLFKKINAQKGVLFSEDQILDWFVQICLALKHIHDRKILHRDIKSQNIFLTKDGTIQLGDFGIARVLNSTVELARTCIGTPYYLSPEICQNRPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIISGSFPPVSVHYSYDLRNLLSQLFKRNPRDRPSVNSILEKNFIAKRVEKFLTPQLIAEEFSHKVFHKFGPHAGPAKRPAQGHISASIAPVQKITKPAAKYGVPLAMKKCYDAPKKLHEKKSLIKLRQISLLKAEEMRRLEKERMERINRAREQGWRNVLGSGGSGDVKAPYYGGGGGVGPFPASFRGQYEHYHAIFDQMQKETFKVTKERQAKWRAEVQGQEAVERGIPPGVRPGVPKGPAGHHHLPDADAIRKKVKRLKEVSKQASANRQKGWLPAERAKQVEEFWQRKREAMQNKARAEGHMGTLQNVADIYGGRPMSARGRKSRNKEEEEYLARLRQIRLQNFNERQQIKAKLRGEKNEAPSSDGQESSEEAEMKRKKIEAQKAQANARAAVLKEQLERKRKEAYEREKRAWEEHLIAKGVKNPNVPFHVGAAGHTPVHGIQEPGVSPPKQQLPVKPISMTSALKEVGVDGNVTAAQEVKEEIKKPDLTMQNKRQILKRLNQNLKAQEDEKGRKDDKNISESDGSEDGKEEQEDNHPLLGDRKRWASRASDLVVPLAQLSMEDSLSGTDRQTMGEVIRLDIGEPRRKVWGKSPTDSVLKILGEAELQLPTELLEEIDVINEPDDLEAVMLKETEEDKKHQSKEFPIAVNEVWVKEKQDEAQQDARNEAAFEKEVVLDEVQSQNEAPEETCSSDSLQPEPLFQRVIQPPAVPTASPVLSSQSSQEEPFVPRSRSISPAKNKGKSSLFFGLSTGLFDANNPKMLRTCSLPDLYKLYRTLVDVPSIADVQHQHSLEIDDTEDEQAKEGPSDSEDIMFGDADTDLQELRASMEQLLREQPSEELSEEEESTLKANVIKCMTNGTELDEADENNPSSESALNEEWQSDNSDGDIASECEECDSVFSHLEELRYNLEQEIGFEKLIEVYNKIKAIHEDEDENIDICSSIVQTILGNEHKHLYAKVLHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_015285380.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus NIMA related kinase 1 (NEK1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015285380.2

ORF Insert Sequence:

ATGGATAAAT ACATTAAAGT GCAAAAAATT GGAGAAGGAT CCTTTGGGAA AGCATTTCTT 
GTTAAAGATA AAGAAAATAG CCAGCAGTAT GTTATTAAAG AAATTAACAT ATCTAAGATG 
TCAAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TACTGGCCAA CATGAAACAT 
CCAAATATTG TCCTGTATAG GGAGTCGTTT GAAGAAAATG GTTGTCTCTA CATAGTGATG 
GATTACTGTG AAGGAGGAGA TCTGTTCAAA AAAATAAATG CCCAGAAAGG AGTCCTGTTC 
TCTGAGGATC AGATTCTTGA TTGGTTTGTA CAGATCTGTT TGGCACTGAA ACATATACAT 
GATAGAAAAA TCTTACATCG CGACATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA 
ACAATACAGC TGGGAGATTT TGGAATCGCG AGAGTTCTGA ACAGTACTGT GGAATTGGCT 
CGCACTTGCA TAGGAACACC ATACTATTTA TCACCTGAAA TATGCCAGAA CAGGCCTTAC 
AACAATAAAA GTGATATCTG GGCCCTTGGT TGCGTTCTCT ATGAAATGTG CACACTTAAG 
CATGCATTTG AAGCTGGTAA CATGAAGAAC TTGGTACTGA AGATAATCTC TGGATCTTTC 
CCTCCTGTTT CTGTGCATTA TTCCTATGAC CTACGTAATC TACTGTCACA GTTATTTAAA 
AGGAATCCTA GGGATAGACC ATCAGTGAAC TCCATATTGG AGAAAAATTT CATAGCCAAA 
CGGGTTGAAA AATTTCTCAC ACCGCAGCTT ATTGCAGAAG AATTCAGTCA CAAAGTGTTT 
CACAAGTTTG GACCACATGC TGGACCAGCT AAAAGACCAG CTCAAGGACA TATCTCGGCT 
TCAATTGCAC CAGTGCAGAA AATTACCAAA CCTGCTGCAA AATACGGAGT GCCTTTAGCG 
ATGAAGAAGT GTTATGACGC ACCCAAAAAG CTTCATGAGA AAAAGTCATT GATTAAACTT 
AGGCAGATCA GTTTACTGAA GGCAGAGGAA ATGAGAAGAT TGGAAAAAGA AAGGATGGAA 
CGAATAAACA GAGCCAGGGA GCAAGGATGG AGGAATGTGC TTGGTTCAGG TGGAAGTGGT 
GATGTTAAGG CACCATATTA TGGCGGTGGA GGTGGTGTTG GTCCTTTTCC TGCCTCTTTC 
AGAGGACAGT ATGAGCACTA TCATGCTATT TTTGACCAGA TGCAAAAGGA AACCTTCAAA 
GTAACTAAAG AGAGGCAAGC CAAATGGAGG GCGGAGGTAC AAGGCCAAGA AGCTGTTGAG 
AGAGGAATTC CACCTGGAGT ACGTCCAGGA GTTCCTAAGG GGCCTGCAGG TCATCACCAT 
TTACCTGATG CTGATGCAAT TAGGAAAAAA GTGAAAAGGT TGAAGGAGGT GTCTAAACAA 
GCCAGTGCAA ACAGGCAGAA AGGATGGCTA CCTGCAGAAA GAGCAAAGCA AGTTGAAGAA 
TTCTGGCAAC GAAAGAGGGA AGCCATGCAA AACAAAGCTC GTGCTGAGGG ACACATGGGT 
ACACTGCAAA ACGTGGCAGA TATCTATGGA GGCAGGCCCA TGTCTGCAAG AGGAAGGAAA 
TCCAGAAACA AGGAAGAAGA GGAATATTTG GCAAGATTAA GACAGATACG ACTACAAAAC 
TTCAATGAGC GTCAACAGAT TAAAGCAAAA CTTCGTGGAG AGAAGAATGA AGCTCCCAGT 
TCTGATGGAC AAGAGTCAAG TGAGGAAGCA GAAATGAAAC GCAAAAAGAT AGAAGCACAG 
AAGGCCCAAG CAAATGCTCG AGCTGCAGTT CTGAAAGAAC AGTTAGAGCG AAAGAGGAAG 
GAGGCATATG AAAGAGAGAA AAGAGCTTGG GAAGAACATC TGATAGCAAA AGGAGTAAAG 
AATCCTAATG TGCCTTTTCA TGTGGGAGCA GCAGGACACA CTCCTGTGCA TGGAATACAA 
GAGCCTGGAG TGTCTCCTCC TAAGCAACAG CTTCCAGTTA AGCCCATCTC CATGACTTCT 
GCTTTGAAAG AAGTTGGCGT GGATGGAAAT GTAACCGCTG CCCAGGAGGT TAAAGAGGAA 
ATAAAAAAGC CAGATCTTAC AATGCAGAAC AAGAGGCAGA TACTGAAAAG ATTGAATCAA 
AATCTTAAAG CTCAGGAAGA TGAGAAAGGA AGAAAGGACG ATAAAAATAT CTCTGAGTCA 
GATGGGTCTG AAGATGGTAA AGAAGAGCAA GAAGATAACC ATCCACTTCT GGGAGATCGC 
AAAAGATGGG CATCAAGGGC ATCAGATTTG GTAGTTCCTT TAGCTCAGTT ATCAATGGAA 
GATTCTCTCT CTGGAACAGA CCGGCAAACT ATGGGGGAAG TAATCAGGCT AGATATTGGG 
GAACCCCGCA GGAAAGTCTG GGGCAAAAGC CCTACTGATT CGGTCCTGAA GATTCTAGGA 
GAAGCGGAGT TGCAGCTACC AACTGAATTA CTGGAAGAAA TAGATGTAAT AAATGAGCCT 
GATGATTTGG AAGCAGTGAT GTTGAAAGAA ACAGAAGAAG ATAAAAAGCA CCAGAGCAAG 
GAATTTCCCA TTGCTGTTAA TGAAGTGTGG GTGAAAGAAA AACAGGATGA GGCACAACAG 
GATGCAAGAA ATGAAGCAGC TTTTGAGAAA GAAGTAGTGC TTGATGAAGT GCAATCACAA 
AATGAAGCTC CAGAAGAAAC TTGTTCCAGT GACTCTCTGC AACCTGAACC GTTATTCCAG 
AGAGTAATAC AGCCCCCAGC TGTACCTACA GCCTCACCAG TTCTATCATC TCAGTCTTCC 
CAAGAAGAGC CTTTTGTACC TCGTTCACGT TCCATATCGC CAGCAAAAAA TAAAGGGAAA 
AGTTCATTGT TTTTTGGACT TTCTACAGGT CTGTTTGATG CAAACAACCC AAAGATGTTG 
AGAACGTGTT CACTCCCGGA TCTTTACAAA CTGTACAGAA CCTTAGTTGA TGTTCCCAGC 
ATAGCAGATG TACAGCATCA ACACAGTCTT GAAATAGATG ATACGGAAGA TGAGCAAGCC 
AAAGAAGGAC CCTCTGATTC TGAGGATATC ATGTTTGGGG ACGCCGATAC AGATTTGCAG 
GAACTCCGGG CCTCAATGGA ACAATTACTT AGGGAGCAGC CTAGTGAGGA ATTAAGTGAA 
GAGGAGGAAT CTACTTTAAA GGCTAACGTC ATCAAATGCA TGACAAATGG TACAGAGCTG 
GATGAAGCAG ATGAAAATAA CCCCAGCAGT GAAAGTGCAC TTAATGAAGA ATGGCAGTCA 
GATAATAGTG ATGGAGATAT TGCCAGTGAA TGTGAAGAAT GTGATAGTGT CTTCAGCCAT 
TTGGAAGAGC TGAGATACAA CCTGGAACAG GAAATAGGCT TTGAGAAATT AATTGAAGTT 
TATAATAAAA TAAAGGCCAT ACATGAAGAT GAAGATGAAA ATATTGATAT TTGTTCAAGC 
ATAGTTCAGA CTATTCTGGG AAATGAACAC AAACACTTAT ATGCCAAAGT ACTTCACCTG 
GTCATGGCGG ATGGAGCCTA CCAGGAAGAT AATGACGAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_015285383.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015140869.1 Gallus gallus NIMA-related kinase 1 (NEK1), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OGa34989
Clone ID Related Accession (Same CDS sequence)	XM_015285383.1 , XM_015285383.2
Accession Version	XM_015285383.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3117bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	serine/threonine-protein kinase Nek1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455855.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCACAC TTAAGCATGC ATTTGAAGCT GGTAACATGA AGAACTTGGT ACTGAAGATA 
ATCTCTGGAT CTTTCCCTCC TGTTTCTGTG CATTATTCCT ATGACCTACG TAATCTACTG 
TCACAGTTAT TTAAAAGGAA TCCTAGGGAT AGACCATCAG TGAACTCCAT ATTGGAGAAA 
AATTTCATAG CCAAACGGGT TGAAAAATTT CTCACACCGC AGCTTATTGC AGAAGAATTC 
AGTCACAAAG TGTTTCACAA GTTTGGACCA CATGCTGGAC CAGCTAAAAG ACCAGCTCAA 
GGACATATCT CGGCTTCAAT TGCACCAGTG CAGAAAATTA CCAAACCTGC TGCAAAATAC 
GGAGTGCCTT TAGCGATGAA GAAGTGTTAT GACGCACCCA AAAAGCTTCA TGAGAAAAAG 
TCATTGATTA AACTTAGGCA GGCCTTTCCA ACTCCAAAGA AGAAAATTAT TCCAGGAGAA 
GAAAGGAGGA AAATGTTTGA GGAACCTTCA AAAAAGAAAA GGTTAGAATT GCCTGAAAAA 
GAAAAACGCC AGAGAGATCA GATCAGTTTA CTGAAGGCAG AGGAAATGAG AAGATTGGAA 
AAAGAAAGGA TGGAACGAAT AAACAGAGCC AGGGAGCAAG GATGGAGGAA TGTGCTTGGT 
TCAGGTGGAA GTGGTGATGT TAAGGCACCA TATTATGGCG GTGGAGGTGG TGTTGGTCCT 
TTTCCTGCCT CTTTCAGAGG ACAGTATGAG CACTATCATG CTATTTTTGA CCAGATGCAA 
AAGGAAACCT TCAAAGTAAC TAAAGAGAGG CAAGCCAAAT GGAGGGCGGA GGTACAAGGC 
CAAGAAGCTG TTGAGAGAGG AATTCCACCT GGAGTACGTC CAGGAGTTCC TAAGGGGCCT 
GCAGGTCATC ACCATTTACC TGATGCTGAT GCAATTAGGA AAAAAGTGAA AAGGTTGAAG 
GAGGTGTCTA AACAAGCCAG TGCAAACAGG CAGAAAGGAT GGCTACCTGC AGAAAGAGCA 
AAGCAAGTTG AAGAATTCTG GCAACGAAAG AGGGAAGCCA TGCAAAACAA AGCTCGTGCT 
GAGGGACACA TGGGTACACT GCAAAACGTG GCAGATATCT ATGGAGGCAG GCCCATGTCT 
GCAAGAGGAA GGAAATCCAG AAACAAGGAA GAAGAGGAAT ATTTGGCAAG ATTAAGACAG 
ATACGACTAC AAAACTTCAA TGAGCGTCAA CAGATTAAAG CAAAACTTCG TGGAGAGAAG 
AATGAAGCTC CCAGTTCTGA TGGACAAGAG TCAAGTGAGG AAGCAGAAAT GAAACGCAAA 
AAGATAGAAG CACAGAAGGC CCAAGCAAAT GCTCGAGCTG CAGTTCTGAA AGAACAGTTA 
GAGCGAAAGA GGAAGGAGGC ATATGAAAGA GAGAAAAGAG CTTGGGAAGA ACATCTGATA 
GCAAAAGGAG TAAAGAATCC TAATGTGCCT TTTCATGTGG GAGCAGCAGG ACACACTCCT 
GTGCATGGAA TACAAGAGCC TGGAGTGTCT CCTCCTAAGC AACAGCTTCC AGTTAAGCCC 
ATCTCCATGA CTTCTGCTTT GAAAGAAGTT GGCGTGGATG GAAATGTAAC CGCTGCCCAG 
GAGGTTAAAG AGGAAATAAA AAAGCCAGAT CTTACAATGC AGAACAAGAG GCAGATACTG 
AAAAGATTGA ATCAAAATCT TAAAGCTCAG GAAGATGAGA AAGGAAGAAA GGACGATAAA 
AATATCTCTG AGTCAGATGG GTCTGAAGAT GGTAAAGAAG AGCAAGAAGA TAACCATCCA 
CTTCTGGGAG ATCGCAAAAG ATGGGCATCA AGGGCATCAG ATTTGGTAGT TCCTTTAGCT 
CAGTTATCAA TGGAAGATTC TCTCTCTGGA ACAGACCGGC AAACTATGGG GGAAGTAATC 
AGGCTAGATA TTGGGGAACC CCGCAGGAAA GTCTGGGGCA AAAGCCCTAC TGATTCGGTC 
CTGAAGATTC TAGGAGAAGC GGAGTTGCAG CTACCAACTG AATTACTGGA AGAAATAGAT 
GTAATAAATG AGCCTGATGA TTTGGAAGCA GTGATGTTGA AAGAAACAGA AGAAGATAAA 
AAGCACCAGA GCAAGGAATT TCCCATTGCT GTTAATGAAG TGTGGGTGAA AGAAAAACAG 
GATGAGGCAC AACAGGATGC AAGAAATGAA GCAGCTTTTG AGAAAGAAGT AGTGCTTGAT 
GAAGTGCAAT CACAAAATGA AGCTCCAGAA GAAACTTGTT CCAGTGACTC TCTGCAACCT 
GAACCGTTAT TCCAGAGAGT AATACAGCCC CCAGCTGTAC CTACAGCCTC ACCAGTTCTA 
TCATCTCAGT CTTCCCAAGA AGAGCCTTTT GTACCTCGTT CACGTTCCAT ATCGCCAGCA 
AAAAATAAAG GGAAAAGTTC ATTGTTTTTT GGACTTTCTA CAGGTCTGTT TGATGCAAAC 
AACCCAAAGA TGTTGAGAAC GTGTTCACTC CCGGATCTTT ACAAACTGTA CAGAACCTTA 
GTTGATGTTC CCAGCATAGC AGATGTACAG CATCAACACA GTCTTGAAAT AGATGATACG 
GAAGATGAGC AAGCCAAAGA AGGACCCTCT GATTCTGAGG ATATCATGTT TGGGGACGCC 
GATACAGATT TGCAGGAACT CCGGGCCTCA ATGGAACAAT TACTTAGGGA GCAGCCTAGT 
GAGGAATTAA GTGAAGAGGA GGAATCTACT TTAAAGGCTA ACGTCATCAA ATGCATGACA 
AATGGTACAG AGCTGGATGA AGCAGATGAA AATAACCCCA GCAGTGAAAG TGCACTTAAT 
GAAGAATGGC AGTCAGATAA TAGTGATGGA GATATTGCCA GTGAATGTGA AGAATGTGAT 
AGTGTCTTCA GCCATTTGGA AGAGCTGAGA TACAACCTGG AACAGGAAAT AGGCTTTGAG 
AAATTAATTG AAGTTTATAA TAAAATAAAG GCCATACATG AAGATGAAGA TGAAAATATT 
GATATTTGTT CAAGCATAGT TCAGACTATT CTGGGAAATG AACACAAACA CTTATATGCC 
AAAGTACTTC ACCTGGTCAT GGCGGATGGA GCCTACCAGG AAGATAATGA CGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015140869.1
CDS	106..3222
Translation	MCTLKHAFEAGNMKNLVLKIISGSFPPVSVHYSYDLRNLLSQLFKRNPRDRPSVNSILEKNFIAKRVEKFLTPQLIAEEFSHKVFHKFGPHAGPAKRPAQGHISASIAPVQKITKPAAKYGVPLAMKKCYDAPKKLHEKKSLIKLRQAFPTPKKKIIPGEERRKMFEEPSKKKRLELPEKEKRQRDQISLLKAEEMRRLEKERMERINRAREQGWRNVLGSGGSGDVKAPYYGGGGGVGPFPASFRGQYEHYHAIFDQMQKETFKVTKERQAKWRAEVQGQEAVERGIPPGVRPGVPKGPAGHHHLPDADAIRKKVKRLKEVSKQASANRQKGWLPAERAKQVEEFWQRKREAMQNKARAEGHMGTLQNVADIYGGRPMSARGRKSRNKEEEEYLARLRQIRLQNFNERQQIKAKLRGEKNEAPSSDGQESSEEAEMKRKKIEAQKAQANARAAVLKEQLERKRKEAYEREKRAWEEHLIAKGVKNPNVPFHVGAAGHTPVHGIQEPGVSPPKQQLPVKPISMTSALKEVGVDGNVTAAQEVKEEIKKPDLTMQNKRQILKRLNQNLKAQEDEKGRKDDKNISESDGSEDGKEEQEDNHPLLGDRKRWASRASDLVVPLAQLSMEDSLSGTDRQTMGEVIRLDIGEPRRKVWGKSPTDSVLKILGEAELQLPTELLEEIDVINEPDDLEAVMLKETEEDKKHQSKEFPIAVNEVWVKEKQDEAQQDARNEAAFEKEVVLDEVQSQNEAPEETCSSDSLQPEPLFQRVIQPPAVPTASPVLSSQSSQEEPFVPRSRSISPAKNKGKSSLFFGLSTGLFDANNPKMLRTCSLPDLYKLYRTLVDVPSIADVQHQHSLEIDDTEDEQAKEGPSDSEDIMFGDADTDLQELRASMEQLLREQPSEELSEEEESTLKANVIKCMTNGTELDEADENNPSSESALNEEWQSDNSDGDIASECEECDSVFSHLEELRYNLEQEIGFEKLIEVYNKIKAIHEDEDENIDICSSIVQTILGNEHKHLYAKVLHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_015285383.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus NIMA-related kinase 1 (NEK1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015285383.1

ORF Insert Sequence:

ATGTGCACAC TTAAGCATGC ATTTGAAGCT GGTAACATGA AGAACTTGGT ACTGAAGATA 
ATCTCTGGAT CTTTCCCTCC TGTTTCTGTG CATTATTCCT ATGACCTACG TAATCTACTG 
TCACAGTTAT TTAAAAGGAA TCCTAGGGAT AGACCATCAG TGAACTCCAT ATTGGAGAAA 
AATTTCATAG CCAAACGGGT TGAAAAATTT CTCACACCGC AGCTTATTGC AGAAGAATTC 
AGTCACAAAG TGTTTCACAA GTTTGGACCA CATGCTGGAC CAGCTAAAAG ACCAGCTCAA 
GGACATATCT CGGCTTCAAT TGCACCAGTG CAGAAAATTA CCAAACCTGC TGCAAAATAC 
GGAGTGCCTT TAGCGATGAA GAAGTGTTAT GACGCACCCA AAAAGCTTCA TGAGAAAAAG 
TCATTGATTA AACTTAGGCA GGCCTTTCCA ACTCCAAAGA AGAAAATTAT TCCAGGAGAA 
GAAAGGAGGA AAATGTTTGA GGAACCTTCA AAAAAGAAAA GGTTAGAATT GCCTGAAAAA 
GAAAAACGCC AGAGAGATCA GATCAGTTTA CTGAAGGCAG AGGAAATGAG AAGATTGGAA 
AAAGAAAGGA TGGAACGAAT AAACAGAGCC AGGGAGCAAG GATGGAGGAA TGTGCTTGGT 
TCAGGTGGAA GTGGTGATGT TAAGGCACCA TATTATGGCG GTGGAGGTGG TGTTGGTCCT 
TTTCCTGCCT CTTTCAGAGG ACAGTATGAG CACTATCATG CTATTTTTGA CCAGATGCAA 
AAGGAAACCT TCAAAGTAAC TAAAGAGAGG CAAGCCAAAT GGAGGGCGGA GGTACAAGGC 
CAAGAAGCTG TTGAGAGAGG AATTCCACCT GGAGTACGTC CAGGAGTTCC TAAGGGGCCT 
GCAGGTCATC ACCATTTACC TGATGCTGAT GCAATTAGGA AAAAAGTGAA AAGGTTGAAG 
GAGGTGTCTA AACAAGCCAG TGCAAACAGG CAGAAAGGAT GGCTACCTGC AGAAAGAGCA 
AAGCAAGTTG AAGAATTCTG GCAACGAAAG AGGGAAGCCA TGCAAAACAA AGCTCGTGCT 
GAGGGACACA TGGGTACACT GCAAAACGTG GCAGATATCT ATGGAGGCAG GCCCATGTCT 
GCAAGAGGAA GGAAATCCAG AAACAAGGAA GAAGAGGAAT ATTTGGCAAG ATTAAGACAG 
ATACGACTAC AAAACTTCAA TGAGCGTCAA CAGATTAAAG CAAAACTTCG TGGAGAGAAG 
AATGAAGCTC CCAGTTCTGA TGGACAAGAG TCAAGTGAGG AAGCAGAAAT GAAACGCAAA 
AAGATAGAAG CACAGAAGGC CCAAGCAAAT GCTCGAGCTG CAGTTCTGAA AGAACAGTTA 
GAGCGAAAGA GGAAGGAGGC ATATGAAAGA GAGAAAAGAG CTTGGGAAGA ACATCTGATA 
GCAAAAGGAG TAAAGAATCC TAATGTGCCT TTTCATGTGG GAGCAGCAGG ACACACTCCT 
GTGCATGGAA TACAAGAGCC TGGAGTGTCT CCTCCTAAGC AACAGCTTCC AGTTAAGCCC 
ATCTCCATGA CTTCTGCTTT GAAAGAAGTT GGCGTGGATG GAAATGTAAC CGCTGCCCAG 
GAGGTTAAAG AGGAAATAAA AAAGCCAGAT CTTACAATGC AGAACAAGAG GCAGATACTG 
AAAAGATTGA ATCAAAATCT TAAAGCTCAG GAAGATGAGA AAGGAAGAAA GGACGATAAA 
AATATCTCTG AGTCAGATGG GTCTGAAGAT GGTAAAGAAG AGCAAGAAGA TAACCATCCA 
CTTCTGGGAG ATCGCAAAAG ATGGGCATCA AGGGCATCAG ATTTGGTAGT TCCTTTAGCT 
CAGTTATCAA TGGAAGATTC TCTCTCTGGA ACAGACCGGC AAACTATGGG GGAAGTAATC 
AGGCTAGATA TTGGGGAACC CCGCAGGAAA GTCTGGGGCA AAAGCCCTAC TGATTCGGTC 
CTGAAGATTC TAGGAGAAGC GGAGTTGCAG CTACCAACTG AATTACTGGA AGAAATAGAT 
GTAATAAATG AGCCTGATGA TTTGGAAGCA GTGATGTTGA AAGAAACAGA AGAAGATAAA 
AAGCACCAGA GCAAGGAATT TCCCATTGCT GTTAATGAAG TGTGGGTGAA AGAAAAACAG 
GATGAGGCAC AACAGGATGC AAGAAATGAA GCAGCTTTTG AGAAAGAAGT AGTGCTTGAT 
GAAGTGCAAT CACAAAATGA AGCTCCAGAA GAAACTTGTT CCAGTGACTC TCTGCAACCT 
GAACCGTTAT TCCAGAGAGT AATACAGCCC CCAGCTGTAC CTACAGCCTC ACCAGTTCTA 
TCATCTCAGT CTTCCCAAGA AGAGCCTTTT GTACCTCGTT CACGTTCCAT ATCGCCAGCA 
AAAAATAAAG GGAAAAGTTC ATTGTTTTTT GGACTTTCTA CAGGTCTGTT TGATGCAAAC 
AACCCAAAGA TGTTGAGAAC GTGTTCACTC CCGGATCTTT ACAAACTGTA CAGAACCTTA 
GTTGATGTTC CCAGCATAGC AGATGTACAG CATCAACACA GTCTTGAAAT AGATGATACG 
GAAGATGAGC AAGCCAAAGA AGGACCCTCT GATTCTGAGG ATATCATGTT TGGGGACGCC 
GATACAGATT TGCAGGAACT CCGGGCCTCA ATGGAACAAT TACTTAGGGA GCAGCCTAGT 
GAGGAATTAA GTGAAGAGGA GGAATCTACT TTAAAGGCTA ACGTCATCAA ATGCATGACA 
AATGGTACAG AGCTGGATGA AGCAGATGAA AATAACCCCA GCAGTGAAAG TGCACTTAAT 
GAAGAATGGC AGTCAGATAA TAGTGATGGA GATATTGCCA GTGAATGTGA AGAATGTGAT 
AGTGTCTTCA GCCATTTGGA AGAGCTGAGA TACAACCTGG AACAGGAAAT AGGCTTTGAG 
AAATTAATTG AAGTTTATAA TAAAATAAAG GCCATACATG AAGATGAAGA TGAAAATATT 
GATATTTGTT CAAGCATAGT TCAGACTATT CTGGGAAATG AACACAAACA CTTATATGCC 
AAAGTACTTC ACCTGGTCAT GGCGGATGGA GCCTACCAGG AAGATAATGA CGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_015285383.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015140869.1 Gallus gallus NIMA related kinase 1 (NEK1), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OGa34989
Clone ID Related Accession (Same CDS sequence)	XM_015285383.1 , XM_015285383.2
Accession Version	XM_015285383.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3117bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	serine/threonine-protein kinase Nek1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015285383.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCACAC TTAAGCATGC ATTTGAAGCT GGTAACATGA AGAACTTGGT ACTGAAGATA 
ATCTCTGGAT CTTTCCCTCC TGTTTCTGTG CATTATTCCT ATGACCTACG TAATCTACTG 
TCACAGTTAT TTAAAAGGAA TCCTAGGGAT AGACCATCAG TGAACTCCAT ATTGGAGAAA 
AATTTCATAG CCAAACGGGT TGAAAAATTT CTCACACCGC AGCTTATTGC AGAAGAATTC 
AGTCACAAAG TGTTTCACAA GTTTGGACCA CATGCTGGAC CAGCTAAAAG ACCAGCTCAA 
GGACATATCT CGGCTTCAAT TGCACCAGTG CAGAAAATTA CCAAACCTGC TGCAAAATAC 
GGAGTGCCTT TAGCGATGAA GAAGTGTTAT GACGCACCCA AAAAGCTTCA TGAGAAAAAG 
TCATTGATTA AACTTAGGCA GGCCTTTCCA ACTCCAAAGA AGAAAATTAT TCCAGGAGAA 
GAAAGGAGGA AAATGTTTGA GGAACCTTCA AAAAAGAAAA GGTTAGAATT GCCTGAAAAA 
GAAAAACGCC AGAGAGATCA GATCAGTTTA CTGAAGGCAG AGGAAATGAG AAGATTGGAA 
AAAGAAAGGA TGGAACGAAT AAACAGAGCC AGGGAGCAAG GATGGAGGAA TGTGCTTGGT 
TCAGGTGGAA GTGGTGATGT TAAGGCACCA TATTATGGCG GTGGAGGTGG TGTTGGTCCT 
TTTCCTGCCT CTTTCAGAGG ACAGTATGAG CACTATCATG CTATTTTTGA CCAGATGCAA 
AAGGAAACCT TCAAAGTAAC TAAAGAGAGG CAAGCCAAAT GGAGGGCGGA GGTACAAGGC 
CAAGAAGCTG TTGAGAGAGG AATTCCACCT GGAGTACGTC CAGGAGTTCC TAAGGGGCCT 
GCAGGTCATC ACCATTTACC TGATGCTGAT GCAATTAGGA AAAAAGTGAA AAGGTTGAAG 
GAGGTGTCTA AACAAGCCAG TGCAAACAGG CAGAAAGGAT GGCTACCTGC AGAAAGAGCA 
AAGCAAGTTG AAGAATTCTG GCAACGAAAG AGGGAAGCCA TGCAAAACAA AGCTCGTGCT 
GAGGGACACA TGGGTACACT GCAAAACGTG GCAGATATCT ATGGAGGCAG GCCCATGTCT 
GCAAGAGGAA GGAAATCCAG AAACAAGGAA GAAGAGGAAT ATTTGGCAAG ATTAAGACAG 
ATACGACTAC AAAACTTCAA TGAGCGTCAA CAGATTAAAG CAAAACTTCG TGGAGAGAAG 
AATGAAGCTC CCAGTTCTGA TGGACAAGAG TCAAGTGAGG AAGCAGAAAT GAAACGCAAA 
AAGATAGAAG CACAGAAGGC CCAAGCAAAT GCTCGAGCTG CAGTTCTGAA AGAACAGTTA 
GAGCGAAAGA GGAAGGAGGC ATATGAAAGA GAGAAAAGAG CTTGGGAAGA ACATCTGATA 
GCAAAAGGAG TAAAGAATCC TAATGTGCCT TTTCATGTGG GAGCAGCAGG ACACACTCCT 
GTGCATGGAA TACAAGAGCC TGGAGTGTCT CCTCCTAAGC AACAGCTTCC AGTTAAGCCC 
ATCTCCATGA CTTCTGCTTT GAAAGAAGTT GGCGTGGATG GAAATGTAAC CGCTGCCCAG 
GAGGTTAAAG AGGAAATAAA AAAGCCAGAT CTTACAATGC AGAACAAGAG GCAGATACTG 
AAAAGATTGA ATCAAAATCT TAAAGCTCAG GAAGATGAGA AAGGAAGAAA GGACGATAAA 
AATATCTCTG AGTCAGATGG GTCTGAAGAT GGTAAAGAAG AGCAAGAAGA TAACCATCCA 
CTTCTGGGAG ATCGCAAAAG ATGGGCATCA AGGGCATCAG ATTTGGTAGT TCCTTTAGCT 
CAGTTATCAA TGGAAGATTC TCTCTCTGGA ACAGACCGGC AAACTATGGG GGAAGTAATC 
AGGCTAGATA TTGGGGAACC CCGCAGGAAA GTCTGGGGCA AAAGCCCTAC TGATTCGGTC 
CTGAAGATTC TAGGAGAAGC GGAGTTGCAG CTACCAACTG AATTACTGGA AGAAATAGAT 
GTAATAAATG AGCCTGATGA TTTGGAAGCA GTGATGTTGA AAGAAACAGA AGAAGATAAA 
AAGCACCAGA GCAAGGAATT TCCCATTGCT GTTAATGAAG TGTGGGTGAA AGAAAAACAG 
GATGAGGCAC AACAGGATGC AAGAAATGAA GCAGCTTTTG AGAAAGAAGT AGTGCTTGAT 
GAAGTGCAAT CACAAAATGA AGCTCCAGAA GAAACTTGTT CCAGTGACTC TCTGCAACCT 
GAACCGTTAT TCCAGAGAGT AATACAGCCC CCAGCTGTAC CTACAGCCTC ACCAGTTCTA 
TCATCTCAGT CTTCCCAAGA AGAGCCTTTT GTACCTCGTT CACGTTCCAT ATCGCCAGCA 
AAAAATAAAG GGAAAAGTTC ATTGTTTTTT GGACTTTCTA CAGGTCTGTT TGATGCAAAC 
AACCCAAAGA TGTTGAGAAC GTGTTCACTC CCGGATCTTT ACAAACTGTA CAGAACCTTA 
GTTGATGTTC CCAGCATAGC AGATGTACAG CATCAACACA GTCTTGAAAT AGATGATACG 
GAAGATGAGC AAGCCAAAGA AGGACCCTCT GATTCTGAGG ATATCATGTT TGGGGACGCC 
GATACAGATT TGCAGGAACT CCGGGCCTCA ATGGAACAAT TACTTAGGGA GCAGCCTAGT 
GAGGAATTAA GTGAAGAGGA GGAATCTACT TTAAAGGCTA ACGTCATCAA ATGCATGACA 
AATGGTACAG AGCTGGATGA AGCAGATGAA AATAACCCCA GCAGTGAAAG TGCACTTAAT 
GAAGAATGGC AGTCAGATAA TAGTGATGGA GATATTGCCA GTGAATGTGA AGAATGTGAT 
AGTGTCTTCA GCCATTTGGA AGAGCTGAGA TACAACCTGG AACAGGAAAT AGGCTTTGAG 
AAATTAATTG AAGTTTATAA TAAAATAAAG GCCATACATG AAGATGAAGA TGAAAATATT 
GATATTTGTT CAAGCATAGT TCAGACTATT CTGGGAAATG AACACAAACA CTTATATGCC 
AAAGTACTTC ACCTGGTCAT GGCGGATGGA GCCTACCAGG AAGATAATGA CGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015140869.1
CDS	100..3216
Translation	MCTLKHAFEAGNMKNLVLKIISGSFPPVSVHYSYDLRNLLSQLFKRNPRDRPSVNSILEKNFIAKRVEKFLTPQLIAEEFSHKVFHKFGPHAGPAKRPAQGHISASIAPVQKITKPAAKYGVPLAMKKCYDAPKKLHEKKSLIKLRQAFPTPKKKIIPGEERRKMFEEPSKKKRLELPEKEKRQRDQISLLKAEEMRRLEKERMERINRAREQGWRNVLGSGGSGDVKAPYYGGGGGVGPFPASFRGQYEHYHAIFDQMQKETFKVTKERQAKWRAEVQGQEAVERGIPPGVRPGVPKGPAGHHHLPDADAIRKKVKRLKEVSKQASANRQKGWLPAERAKQVEEFWQRKREAMQNKARAEGHMGTLQNVADIYGGRPMSARGRKSRNKEEEEYLARLRQIRLQNFNERQQIKAKLRGEKNEAPSSDGQESSEEAEMKRKKIEAQKAQANARAAVLKEQLERKRKEAYEREKRAWEEHLIAKGVKNPNVPFHVGAAGHTPVHGIQEPGVSPPKQQLPVKPISMTSALKEVGVDGNVTAAQEVKEEIKKPDLTMQNKRQILKRLNQNLKAQEDEKGRKDDKNISESDGSEDGKEEQEDNHPLLGDRKRWASRASDLVVPLAQLSMEDSLSGTDRQTMGEVIRLDIGEPRRKVWGKSPTDSVLKILGEAELQLPTELLEEIDVINEPDDLEAVMLKETEEDKKHQSKEFPIAVNEVWVKEKQDEAQQDARNEAAFEKEVVLDEVQSQNEAPEETCSSDSLQPEPLFQRVIQPPAVPTASPVLSSQSSQEEPFVPRSRSISPAKNKGKSSLFFGLSTGLFDANNPKMLRTCSLPDLYKLYRTLVDVPSIADVQHQHSLEIDDTEDEQAKEGPSDSEDIMFGDADTDLQELRASMEQLLREQPSEELSEEEESTLKANVIKCMTNGTELDEADENNPSSESALNEEWQSDNSDGDIASECEECDSVFSHLEELRYNLEQEIGFEKLIEVYNKIKAIHEDEDENIDICSSIVQTILGNEHKHLYAKVLHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_015285383.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus NIMA related kinase 1 (NEK1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015285383.2

ORF Insert Sequence:

ATGTGCACAC TTAAGCATGC ATTTGAAGCT GGTAACATGA AGAACTTGGT ACTGAAGATA 
ATCTCTGGAT CTTTCCCTCC TGTTTCTGTG CATTATTCCT ATGACCTACG TAATCTACTG 
TCACAGTTAT TTAAAAGGAA TCCTAGGGAT AGACCATCAG TGAACTCCAT ATTGGAGAAA 
AATTTCATAG CCAAACGGGT TGAAAAATTT CTCACACCGC AGCTTATTGC AGAAGAATTC 
AGTCACAAAG TGTTTCACAA GTTTGGACCA CATGCTGGAC CAGCTAAAAG ACCAGCTCAA 
GGACATATCT CGGCTTCAAT TGCACCAGTG CAGAAAATTA CCAAACCTGC TGCAAAATAC 
GGAGTGCCTT TAGCGATGAA GAAGTGTTAT GACGCACCCA AAAAGCTTCA TGAGAAAAAG 
TCATTGATTA AACTTAGGCA GGCCTTTCCA ACTCCAAAGA AGAAAATTAT TCCAGGAGAA 
GAAAGGAGGA AAATGTTTGA GGAACCTTCA AAAAAGAAAA GGTTAGAATT GCCTGAAAAA 
GAAAAACGCC AGAGAGATCA GATCAGTTTA CTGAAGGCAG AGGAAATGAG AAGATTGGAA 
AAAGAAAGGA TGGAACGAAT AAACAGAGCC AGGGAGCAAG GATGGAGGAA TGTGCTTGGT 
TCAGGTGGAA GTGGTGATGT TAAGGCACCA TATTATGGCG GTGGAGGTGG TGTTGGTCCT 
TTTCCTGCCT CTTTCAGAGG ACAGTATGAG CACTATCATG CTATTTTTGA CCAGATGCAA 
AAGGAAACCT TCAAAGTAAC TAAAGAGAGG CAAGCCAAAT GGAGGGCGGA GGTACAAGGC 
CAAGAAGCTG TTGAGAGAGG AATTCCACCT GGAGTACGTC CAGGAGTTCC TAAGGGGCCT 
GCAGGTCATC ACCATTTACC TGATGCTGAT GCAATTAGGA AAAAAGTGAA AAGGTTGAAG 
GAGGTGTCTA AACAAGCCAG TGCAAACAGG CAGAAAGGAT GGCTACCTGC AGAAAGAGCA 
AAGCAAGTTG AAGAATTCTG GCAACGAAAG AGGGAAGCCA TGCAAAACAA AGCTCGTGCT 
GAGGGACACA TGGGTACACT GCAAAACGTG GCAGATATCT ATGGAGGCAG GCCCATGTCT 
GCAAGAGGAA GGAAATCCAG AAACAAGGAA GAAGAGGAAT ATTTGGCAAG ATTAAGACAG 
ATACGACTAC AAAACTTCAA TGAGCGTCAA CAGATTAAAG CAAAACTTCG TGGAGAGAAG 
AATGAAGCTC CCAGTTCTGA TGGACAAGAG TCAAGTGAGG AAGCAGAAAT GAAACGCAAA 
AAGATAGAAG CACAGAAGGC CCAAGCAAAT GCTCGAGCTG CAGTTCTGAA AGAACAGTTA 
GAGCGAAAGA GGAAGGAGGC ATATGAAAGA GAGAAAAGAG CTTGGGAAGA ACATCTGATA 
GCAAAAGGAG TAAAGAATCC TAATGTGCCT TTTCATGTGG GAGCAGCAGG ACACACTCCT 
GTGCATGGAA TACAAGAGCC TGGAGTGTCT CCTCCTAAGC AACAGCTTCC AGTTAAGCCC 
ATCTCCATGA CTTCTGCTTT GAAAGAAGTT GGCGTGGATG GAAATGTAAC CGCTGCCCAG 
GAGGTTAAAG AGGAAATAAA AAAGCCAGAT CTTACAATGC AGAACAAGAG GCAGATACTG 
AAAAGATTGA ATCAAAATCT TAAAGCTCAG GAAGATGAGA AAGGAAGAAA GGACGATAAA 
AATATCTCTG AGTCAGATGG GTCTGAAGAT GGTAAAGAAG AGCAAGAAGA TAACCATCCA 
CTTCTGGGAG ATCGCAAAAG ATGGGCATCA AGGGCATCAG ATTTGGTAGT TCCTTTAGCT 
CAGTTATCAA TGGAAGATTC TCTCTCTGGA ACAGACCGGC AAACTATGGG GGAAGTAATC 
AGGCTAGATA TTGGGGAACC CCGCAGGAAA GTCTGGGGCA AAAGCCCTAC TGATTCGGTC 
CTGAAGATTC TAGGAGAAGC GGAGTTGCAG CTACCAACTG AATTACTGGA AGAAATAGAT 
GTAATAAATG AGCCTGATGA TTTGGAAGCA GTGATGTTGA AAGAAACAGA AGAAGATAAA 
AAGCACCAGA GCAAGGAATT TCCCATTGCT GTTAATGAAG TGTGGGTGAA AGAAAAACAG 
GATGAGGCAC AACAGGATGC AAGAAATGAA GCAGCTTTTG AGAAAGAAGT AGTGCTTGAT 
GAAGTGCAAT CACAAAATGA AGCTCCAGAA GAAACTTGTT CCAGTGACTC TCTGCAACCT 
GAACCGTTAT TCCAGAGAGT AATACAGCCC CCAGCTGTAC CTACAGCCTC ACCAGTTCTA 
TCATCTCAGT CTTCCCAAGA AGAGCCTTTT GTACCTCGTT CACGTTCCAT ATCGCCAGCA 
AAAAATAAAG GGAAAAGTTC ATTGTTTTTT GGACTTTCTA CAGGTCTGTT TGATGCAAAC 
AACCCAAAGA TGTTGAGAAC GTGTTCACTC CCGGATCTTT ACAAACTGTA CAGAACCTTA 
GTTGATGTTC CCAGCATAGC AGATGTACAG CATCAACACA GTCTTGAAAT AGATGATACG 
GAAGATGAGC AAGCCAAAGA AGGACCCTCT GATTCTGAGG ATATCATGTT TGGGGACGCC 
GATACAGATT TGCAGGAACT CCGGGCCTCA ATGGAACAAT TACTTAGGGA GCAGCCTAGT 
GAGGAATTAA GTGAAGAGGA GGAATCTACT TTAAAGGCTA ACGTCATCAA ATGCATGACA 
AATGGTACAG AGCTGGATGA AGCAGATGAA AATAACCCCA GCAGTGAAAG TGCACTTAAT 
GAAGAATGGC AGTCAGATAA TAGTGATGGA GATATTGCCA GTGAATGTGA AGAATGTGAT 
AGTGTCTTCA GCCATTTGGA AGAGCTGAGA TACAACCTGG AACAGGAAAT AGGCTTTGAG 
AAATTAATTG AAGTTTATAA TAAAATAAAG GCCATACATG AAGATGAAGA TGAAAATATT 
GATATTTGTT CAAGCATAGT TCAGACTATT CTGGGAAATG AACACAAACA CTTATATGCC 
AAAGTACTTC ACCTGGTCAT GGCGGATGGA GCCTACCAGG AAGATAATGA CGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_015285374.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015140860.1 Gallus gallus NIMA related kinase 1 (NEK1), transcript variant X4, mRNA.

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CloneID	OGa07767
Clone ID Related Accession (Same CDS sequence)	XM_015285374.2 , XM_015285375.1 , XM_015285376.1 , XM_420401.5 , XM_420401.6 , XM_015285374.1 , XM_015285376.2 , XM_015285375.2
Accession Version	XM_015285374.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3702bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	serine/threonine-protein kinase Nek1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015285374.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAAAT ACATTAAAGT GCAAAAAATT GGAGAAGGAT CCTTTGGGAA AGCATTTCTT 
GTTAAAGATA AAGAAAATAG CCAGCAGTAT GTTATTAAAG AAATTAACAT ATCTAAGATG 
TCAAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TACTGGCCAA CATGAAACAT 
CCAAATATTG TCCTGTATAG GGAGTCGTTT GAAGAAAATG GTTGTCTCTA CATAGTGATG 
GATTACTGTG AAGGAGGAGA TCTGTTCAAA AAAATAAATG CCCAGAAAGG AGTCCTGTTC 
TCTGAGGATC AGATTCTTGA TTGGTTTGTA CAGATCTGTT TGGCACTGAA ACATATACAT 
GATAGAAAAA TCTTACATCG CGACATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA 
ACAATACAGC TGGGAGATTT TGGAATCGCG AGAGTTCTGA ACAGTACTGT GGAATTGGCT 
CGCACTTGCA TAGGAACACC ATACTATTTA TCACCTGAAA TATGCCAGAA CAGGCCTTAC 
AACAATAAAA GTGATATCTG GGCCCTTGGT TGCGTTCTCT ATGAAATGTG CACACTTAAG 
CATGCATTTG AAGCTGGTAA CATGAAGAAC TTGGTACTGA AGATAATCTC TGGATCTTTC 
CCTCCTGTTT CTGTGCATTA TTCCTATGAC CTACGTAATC TACTGTCACA GTTATTTAAA 
AGGAATCCTA GGGATAGACC ATCAGTGAAC TCCATATTGG AGAAAAATTT CATAGCCAAA 
CGGGTTGAAA AATTTCTCAC ACCGCAGCTT ATTGCAGAAG AATTCAGTCA CAAAGTGTTT 
CACAAGTTTG GACCACATGC TGGACCAGCT AAAAGACCAG CTCAAGGACA TATCTCGGCT 
TCAATTGCAC CAGTGCAGAA AATTACCAAA CCTGCTGCAA AATACGGAGT GCCTTTAGCG 
ATGAAGAAGT GTTATGACGC ACCCAAAAAG CTTCATGAGA AAAAGTCATT GATTAAACTT 
AGGCAGGCCT TTCCAACTCC AAAGAAGAAA ATTATTCCAG GAGAAGAAAG GAGGAAAATG 
TTTGAGGAAC CTTCAAAAAA GAAAAGGTTA GAATTGCCTG AAAAAGAAAA ACGCCAGAGA 
GATCAGATCA GTTTACTGAA GGCAGAGGAA ATGAGAAGAT TGGAAAAAGA AAGGATGGAA 
CGAATAAACA GAGCCAGGGA GCAAGGATGG AGGAATGTGC TTGGTTCAGG TGGAAGTGGT 
GATGTTAAGG CACCATATTA TGGCGGTGGA GGTGGTGTTG GTCCTTTTCC TGCCTCTTTC 
AGAGGACAGT ATGAGCACTA TCATGCTATT TTTGACCAGA TGCAAAAGGA AACCTTCAAA 
GTAACTAAAG AGAGGCAAGC CAAATGGAGG GCGGAGGTAC AAGGCCAAGA AGCTGTTGAG 
AGAGGAATTC CACCTGGAGT ACGTCCAGGA GTTCCTAAGG GGCCTGCAGG TCATCACCAT 
TTACCTGATG CTGATGCAAT TAGGAAAAAA GTGAAAAGGT TGAAGGAGGT GTCTAAACAA 
GCCAGTGCAA ACAGGCAGAA AGGATGGCTA CCTGCAGAAA GAGCAAAGCA AGTTGAAGAA 
TTCTGGCAAC GAAAGAGGGA AGCCATGCAA AACAAAGCTC GTGCTGAGGG ACACATGGGT 
ACACTGCAAA ACGTGGCAGA TATCTATGGA GGCAGGCCCA TGTCTGCAAG AGGAAGGAAA 
TCCAGAAACA AGGAAGAAGA GGAATATTTG GCAAGATTAA GACAGATACG ACTACAAAAC 
TTCAATGAGC GTCAACAGAT TAAAGCAAAA CTTCGTGGAG AGAAGAATGA AGCTCCCAGT 
TCTGATGGAC AAGAGTCAAG TGAGGAAGCA GAAATGAAAC GCAAAAAGAT AGAAGCACAG 
AAGGCCCAAG CAAATGCTCG AGCTGCAGTT CTGAAAGAAC AGTTAGAGCG AAAGAGGAAG 
GAGGCATATG AAAGAGAGAA AAGAGCTTGG GAAGAACATC TGATAGCAAA AGGAGTAAAG 
AATCCTAATG TGCCTTTTCA TGTGGGAGCA GCAGGACACA CTCCTGTGCA TGGAATACAA 
GAGCCTGGAG TGTCTCCTCC TAAGCAACAG CTTCCAGTTA AGCCCATCTC CATGACTTCT 
GCTTTGAAAG AAGTTGGCGT GGATGGAAAT GTAACCGCTG CCCAGGAGGT TAAAGAGGAA 
ATAAAAAAGC CAGATCTTAC AATGCAGAAC AAGAGGCAGA TACTGAAAAG ATTGAATCAA 
AATCTTAAAG CTCAGGAAGA TGAGAAAGGA AGAAAGGACG ATAAAAATAT CTCTGAGTCA 
GATGGGTCTG AAGATGGTAA AGAAGAGCAA GAAGATAACC ATCCACTTCT GGGAGATCGC 
AAAAGATGGG CATCAAGGGC ATCAGATTTG GTAGTTCCTT TAGCTCAGTT ATCAATGGAA 
GATTCTCTCT CTGGAACAGA CCGGCAAACT ATGGGGGAAG TAATCAGGCT AGATATTGGG 
GAACCCCGCA GGAAAGTCTG GGGCAAAAGC CCTACTGATT CGGTCCTGAA GATTCTAGGA 
GAAGCGGAGT TGCAGCTACC AACTGAATTA CTGGAAGAAA TAGATGTAAT AAATGAGCCT 
GATGATTTGG AAGCAGTGAT GTTGAAAGAA ACAGAAGAAG ATAAAAAGCA CCAGAGCAAG 
GAATTTCCCA TTGCTGTTAA TGAAGTGTGG GTGAAAGAAA AACAGGATGA GGCACAACAG 
GATGCAAGAA ATGAAGCAGC TTTTGAGAAA GAAGTAGTGC TTGATGAAGT GCAATCACAA 
AATGAAGCTC CAGAAGAAAC TTGTTCCAGT GACTCTCTGC AACCTGAACC GTTATTCCAG 
AGAGTAATAC AGCCCCCAGC TGTACCTACA GCCTCACCAG TTCTATCATC TCAGTCTTCC 
CAAGAAGAGC CTTTTGTACC TCGTTCACGT TCCATATCGC CAGCAAAAAA TAAAGGGAAA 
AGTTCATTGT TTTTTGGACT TTCTACAGGT CTGTTTGATG CAAACAACCC AAAGATGTTG 
AGAACGTGTT CACTCCCGGA TCTTTACAAA CTGTACAGAA CCTTAGTTGA TGTTCCCAGC 
ATAGCAGATG TACAGCATCA ACACAGTCTT GAAATAGATG ATACGGAAGA TGAGCAAGCC 
AAAGAAGGAC CCTCTGATTC TGAGGATATC ATGTTTGGGG ACGCCGATAC AGATTTGCAG 
GAACTCCGGG CCTCAATGGA ACAATTACTT AGGGAGCAGC CTAGTGAGGA ATTAAGTGAA 
GAGGAGGAAT CTACTTTAAA GGCTAACGTC ATCAAATGCA TGACAAATGG TACAGAGCTG 
GATGAAGCAG ATGAAAATAA CCCCAGCAGT GAAAGTGCAC TTAATGAAGA ATGGCAGTCA 
GATAATAGTG ATGGAGATAT TGCCAGTGAA TGTGAAGAAT GTGATAGTGT CTTCAGCCAT 
TTGGAAGAGC TGAGATACAA CCTGGAACAG GAAATAGGCT TTGAGAAATT AATTGAAGTT 
TATAATAAAA TAAAGGCCAT ACATGAAGAT GAAGATGAAA ATATTGATAT TTGTTCAAGC 
ATAGTTCAGA CTATTCTGGG AAATGAACAC AAACACTTAT ATGCCAAAGT ACTTCACCTG 
GTCATGGCGG ATGGAGCCTA CCAGGAAGAT AATGACGAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015140860.1
CDS	1003..4704
Translation	MDKYIKVQKIGEGSFGKAFLVKDKENSQQYVIKEINISKMSNKEREESRREVAVLANMKHPNIVLYRESFEENGCLYIVMDYCEGGDLFKKINAQKGVLFSEDQILDWFVQICLALKHIHDRKILHRDIKSQNIFLTKDGTIQLGDFGIARVLNSTVELARTCIGTPYYLSPEICQNRPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIISGSFPPVSVHYSYDLRNLLSQLFKRNPRDRPSVNSILEKNFIAKRVEKFLTPQLIAEEFSHKVFHKFGPHAGPAKRPAQGHISASIAPVQKITKPAAKYGVPLAMKKCYDAPKKLHEKKSLIKLRQAFPTPKKKIIPGEERRKMFEEPSKKKRLELPEKEKRQRDQISLLKAEEMRRLEKERMERINRAREQGWRNVLGSGGSGDVKAPYYGGGGGVGPFPASFRGQYEHYHAIFDQMQKETFKVTKERQAKWRAEVQGQEAVERGIPPGVRPGVPKGPAGHHHLPDADAIRKKVKRLKEVSKQASANRQKGWLPAERAKQVEEFWQRKREAMQNKARAEGHMGTLQNVADIYGGRPMSARGRKSRNKEEEEYLARLRQIRLQNFNERQQIKAKLRGEKNEAPSSDGQESSEEAEMKRKKIEAQKAQANARAAVLKEQLERKRKEAYEREKRAWEEHLIAKGVKNPNVPFHVGAAGHTPVHGIQEPGVSPPKQQLPVKPISMTSALKEVGVDGNVTAAQEVKEEIKKPDLTMQNKRQILKRLNQNLKAQEDEKGRKDDKNISESDGSEDGKEEQEDNHPLLGDRKRWASRASDLVVPLAQLSMEDSLSGTDRQTMGEVIRLDIGEPRRKVWGKSPTDSVLKILGEAELQLPTELLEEIDVINEPDDLEAVMLKETEEDKKHQSKEFPIAVNEVWVKEKQDEAQQDARNEAAFEKEVVLDEVQSQNEAPEETCSSDSLQPEPLFQRVIQPPAVPTASPVLSSQSSQEEPFVPRSRSISPAKNKGKSSLFFGLSTGLFDANNPKMLRTCSLPDLYKLYRTLVDVPSIADVQHQHSLEIDDTEDEQAKEGPSDSEDIMFGDADTDLQELRASMEQLLREQPSEELSEEEESTLKANVIKCMTNGTELDEADENNPSSESALNEEWQSDNSDGDIASECEECDSVFSHLEELRYNLEQEIGFEKLIEVYNKIKAIHEDEDENIDICSSIVQTILGNEHKHLYAKVLHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_015285374.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus NIMA related kinase 1 (NEK1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015285374.2

ORF Insert Sequence:

ATGGATAAAT ACATTAAAGT GCAAAAAATT GGAGAAGGAT CCTTTGGGAA AGCATTTCTT 
GTTAAAGATA AAGAAAATAG CCAGCAGTAT GTTATTAAAG AAATTAACAT ATCTAAGATG 
TCAAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TACTGGCCAA CATGAAACAT 
CCAAATATTG TCCTGTATAG GGAGTCGTTT GAAGAAAATG GTTGTCTCTA CATAGTGATG 
GATTACTGTG AAGGAGGAGA TCTGTTCAAA AAAATAAATG CCCAGAAAGG AGTCCTGTTC 
TCTGAGGATC AGATTCTTGA TTGGTTTGTA CAGATCTGTT TGGCACTGAA ACATATACAT 
GATAGAAAAA TCTTACATCG CGACATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA 
ACAATACAGC TGGGAGATTT TGGAATCGCG AGAGTTCTGA ACAGTACTGT GGAATTGGCT 
CGCACTTGCA TAGGAACACC ATACTATTTA TCACCTGAAA TATGCCAGAA CAGGCCTTAC 
AACAATAAAA GTGATATCTG GGCCCTTGGT TGCGTTCTCT ATGAAATGTG CACACTTAAG 
CATGCATTTG AAGCTGGTAA CATGAAGAAC TTGGTACTGA AGATAATCTC TGGATCTTTC 
CCTCCTGTTT CTGTGCATTA TTCCTATGAC CTACGTAATC TACTGTCACA GTTATTTAAA 
AGGAATCCTA GGGATAGACC ATCAGTGAAC TCCATATTGG AGAAAAATTT CATAGCCAAA 
CGGGTTGAAA AATTTCTCAC ACCGCAGCTT ATTGCAGAAG AATTCAGTCA CAAAGTGTTT 
CACAAGTTTG GACCACATGC TGGACCAGCT AAAAGACCAG CTCAAGGACA TATCTCGGCT 
TCAATTGCAC CAGTGCAGAA AATTACCAAA CCTGCTGCAA AATACGGAGT GCCTTTAGCG 
ATGAAGAAGT GTTATGACGC ACCCAAAAAG CTTCATGAGA AAAAGTCATT GATTAAACTT 
AGGCAGGCCT TTCCAACTCC AAAGAAGAAA ATTATTCCAG GAGAAGAAAG GAGGAAAATG 
TTTGAGGAAC CTTCAAAAAA GAAAAGGTTA GAATTGCCTG AAAAAGAAAA ACGCCAGAGA 
GATCAGATCA GTTTACTGAA GGCAGAGGAA ATGAGAAGAT TGGAAAAAGA AAGGATGGAA 
CGAATAAACA GAGCCAGGGA GCAAGGATGG AGGAATGTGC TTGGTTCAGG TGGAAGTGGT 
GATGTTAAGG CACCATATTA TGGCGGTGGA GGTGGTGTTG GTCCTTTTCC TGCCTCTTTC 
AGAGGACAGT ATGAGCACTA TCATGCTATT TTTGACCAGA TGCAAAAGGA AACCTTCAAA 
GTAACTAAAG AGAGGCAAGC CAAATGGAGG GCGGAGGTAC AAGGCCAAGA AGCTGTTGAG 
AGAGGAATTC CACCTGGAGT ACGTCCAGGA GTTCCTAAGG GGCCTGCAGG TCATCACCAT 
TTACCTGATG CTGATGCAAT TAGGAAAAAA GTGAAAAGGT TGAAGGAGGT GTCTAAACAA 
GCCAGTGCAA ACAGGCAGAA AGGATGGCTA CCTGCAGAAA GAGCAAAGCA AGTTGAAGAA 
TTCTGGCAAC GAAAGAGGGA AGCCATGCAA AACAAAGCTC GTGCTGAGGG ACACATGGGT 
ACACTGCAAA ACGTGGCAGA TATCTATGGA GGCAGGCCCA TGTCTGCAAG AGGAAGGAAA 
TCCAGAAACA AGGAAGAAGA GGAATATTTG GCAAGATTAA GACAGATACG ACTACAAAAC 
TTCAATGAGC GTCAACAGAT TAAAGCAAAA CTTCGTGGAG AGAAGAATGA AGCTCCCAGT 
TCTGATGGAC AAGAGTCAAG TGAGGAAGCA GAAATGAAAC GCAAAAAGAT AGAAGCACAG 
AAGGCCCAAG CAAATGCTCG AGCTGCAGTT CTGAAAGAAC AGTTAGAGCG AAAGAGGAAG 
GAGGCATATG AAAGAGAGAA AAGAGCTTGG GAAGAACATC TGATAGCAAA AGGAGTAAAG 
AATCCTAATG TGCCTTTTCA TGTGGGAGCA GCAGGACACA CTCCTGTGCA TGGAATACAA 
GAGCCTGGAG TGTCTCCTCC TAAGCAACAG CTTCCAGTTA AGCCCATCTC CATGACTTCT 
GCTTTGAAAG AAGTTGGCGT GGATGGAAAT GTAACCGCTG CCCAGGAGGT TAAAGAGGAA 
ATAAAAAAGC CAGATCTTAC AATGCAGAAC AAGAGGCAGA TACTGAAAAG ATTGAATCAA 
AATCTTAAAG CTCAGGAAGA TGAGAAAGGA AGAAAGGACG ATAAAAATAT CTCTGAGTCA 
GATGGGTCTG AAGATGGTAA AGAAGAGCAA GAAGATAACC ATCCACTTCT GGGAGATCGC 
AAAAGATGGG CATCAAGGGC ATCAGATTTG GTAGTTCCTT TAGCTCAGTT ATCAATGGAA 
GATTCTCTCT CTGGAACAGA CCGGCAAACT ATGGGGGAAG TAATCAGGCT AGATATTGGG 
GAACCCCGCA GGAAAGTCTG GGGCAAAAGC CCTACTGATT CGGTCCTGAA GATTCTAGGA 
GAAGCGGAGT TGCAGCTACC AACTGAATTA CTGGAAGAAA TAGATGTAAT AAATGAGCCT 
GATGATTTGG AAGCAGTGAT GTTGAAAGAA ACAGAAGAAG ATAAAAAGCA CCAGAGCAAG 
GAATTTCCCA TTGCTGTTAA TGAAGTGTGG GTGAAAGAAA AACAGGATGA GGCACAACAG 
GATGCAAGAA ATGAAGCAGC TTTTGAGAAA GAAGTAGTGC TTGATGAAGT GCAATCACAA 
AATGAAGCTC CAGAAGAAAC TTGTTCCAGT GACTCTCTGC AACCTGAACC GTTATTCCAG 
AGAGTAATAC AGCCCCCAGC TGTACCTACA GCCTCACCAG TTCTATCATC TCAGTCTTCC 
CAAGAAGAGC CTTTTGTACC TCGTTCACGT TCCATATCGC CAGCAAAAAA TAAAGGGAAA 
AGTTCATTGT TTTTTGGACT TTCTACAGGT CTGTTTGATG CAAACAACCC AAAGATGTTG 
AGAACGTGTT CACTCCCGGA TCTTTACAAA CTGTACAGAA CCTTAGTTGA TGTTCCCAGC 
ATAGCAGATG TACAGCATCA ACACAGTCTT GAAATAGATG ATACGGAAGA TGAGCAAGCC 
AAAGAAGGAC CCTCTGATTC TGAGGATATC ATGTTTGGGG ACGCCGATAC AGATTTGCAG 
GAACTCCGGG CCTCAATGGA ACAATTACTT AGGGAGCAGC CTAGTGAGGA ATTAAGTGAA 
GAGGAGGAAT CTACTTTAAA GGCTAACGTC ATCAAATGCA TGACAAATGG TACAGAGCTG 
GATGAAGCAG ATGAAAATAA CCCCAGCAGT GAAAGTGCAC TTAATGAAGA ATGGCAGTCA 
GATAATAGTG ATGGAGATAT TGCCAGTGAA TGTGAAGAAT GTGATAGTGT CTTCAGCCAT 
TTGGAAGAGC TGAGATACAA CCTGGAACAG GAAATAGGCT TTGAGAAATT AATTGAAGTT 
TATAATAAAA TAAAGGCCAT ACATGAAGAT GAAGATGAAA ATATTGATAT TTGTTCAAGC 
ATAGTTCAGA CTATTCTGGG AAATGAACAC AAACACTTAT ATGCCAAAGT ACTTCACCTG 
GTCATGGCGG ATGGAGCCTA CCAGGAAGAT AATGACGAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_015285375.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015140861.1 Gallus gallus NIMA-related kinase 1 (NEK1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OGa07767
Clone ID Related Accession (Same CDS sequence)	XM_015285374.2 , XM_015285375.1 , XM_015285376.1 , XM_420401.5 , XM_420401.6 , XM_015285374.1 , XM_015285376.2 , XM_015285375.2
Accession Version	XM_015285375.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3702bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	serine/threonine-protein kinase Nek1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455855.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAAAT ACATTAAAGT GCAAAAAATT GGAGAAGGAT CCTTTGGGAA AGCATTTCTT 
GTTAAAGATA AAGAAAATAG CCAGCAGTAT GTTATTAAAG AAATTAACAT ATCTAAGATG 
TCAAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TACTGGCCAA CATGAAACAT 
CCAAATATTG TCCTGTATAG GGAGTCGTTT GAAGAAAATG GTTGTCTCTA CATAGTGATG 
GATTACTGTG AAGGAGGAGA TCTGTTCAAA AAAATAAATG CCCAGAAAGG AGTCCTGTTC 
TCTGAGGATC AGATTCTTGA TTGGTTTGTA CAGATCTGTT TGGCACTGAA ACATATACAT 
GATAGAAAAA TCTTACATCG CGACATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA 
ACAATACAGC TGGGAGATTT TGGAATCGCG AGAGTTCTGA ACAGTACTGT GGAATTGGCT 
CGCACTTGCA TAGGAACACC ATACTATTTA TCACCTGAAA TATGCCAGAA CAGGCCTTAC 
AACAATAAAA GTGATATCTG GGCCCTTGGT TGCGTTCTCT ATGAAATGTG CACACTTAAG 
CATGCATTTG AAGCTGGTAA CATGAAGAAC TTGGTACTGA AGATAATCTC TGGATCTTTC 
CCTCCTGTTT CTGTGCATTA TTCCTATGAC CTACGTAATC TACTGTCACA GTTATTTAAA 
AGGAATCCTA GGGATAGACC ATCAGTGAAC TCCATATTGG AGAAAAATTT CATAGCCAAA 
CGGGTTGAAA AATTTCTCAC ACCGCAGCTT ATTGCAGAAG AATTCAGTCA CAAAGTGTTT 
CACAAGTTTG GACCACATGC TGGACCAGCT AAAAGACCAG CTCAAGGACA TATCTCGGCT 
TCAATTGCAC CAGTGCAGAA AATTACCAAA CCTGCTGCAA AATACGGAGT GCCTTTAGCG 
ATGAAGAAGT GTTATGACGC ACCCAAAAAG CTTCATGAGA AAAAGTCATT GATTAAACTT 
AGGCAGGCCT TTCCAACTCC AAAGAAGAAA ATTATTCCAG GAGAAGAAAG GAGGAAAATG 
TTTGAGGAAC CTTCAAAAAA GAAAAGGTTA GAATTGCCTG AAAAAGAAAA ACGCCAGAGA 
GATCAGATCA GTTTACTGAA GGCAGAGGAA ATGAGAAGAT TGGAAAAAGA AAGGATGGAA 
CGAATAAACA GAGCCAGGGA GCAAGGATGG AGGAATGTGC TTGGTTCAGG TGGAAGTGGT 
GATGTTAAGG CACCATATTA TGGCGGTGGA GGTGGTGTTG GTCCTTTTCC TGCCTCTTTC 
AGAGGACAGT ATGAGCACTA TCATGCTATT TTTGACCAGA TGCAAAAGGA AACCTTCAAA 
GTAACTAAAG AGAGGCAAGC CAAATGGAGG GCGGAGGTAC AAGGCCAAGA AGCTGTTGAG 
AGAGGAATTC CACCTGGAGT ACGTCCAGGA GTTCCTAAGG GGCCTGCAGG TCATCACCAT 
TTACCTGATG CTGATGCAAT TAGGAAAAAA GTGAAAAGGT TGAAGGAGGT GTCTAAACAA 
GCCAGTGCAA ACAGGCAGAA AGGATGGCTA CCTGCAGAAA GAGCAAAGCA AGTTGAAGAA 
TTCTGGCAAC GAAAGAGGGA AGCCATGCAA AACAAAGCTC GTGCTGAGGG ACACATGGGT 
ACACTGCAAA ACGTGGCAGA TATCTATGGA GGCAGGCCCA TGTCTGCAAG AGGAAGGAAA 
TCCAGAAACA AGGAAGAAGA GGAATATTTG GCAAGATTAA GACAGATACG ACTACAAAAC 
TTCAATGAGC GTCAACAGAT TAAAGCAAAA CTTCGTGGAG AGAAGAATGA AGCTCCCAGT 
TCTGATGGAC AAGAGTCAAG TGAGGAAGCA GAAATGAAAC GCAAAAAGAT AGAAGCACAG 
AAGGCCCAAG CAAATGCTCG AGCTGCAGTT CTGAAAGAAC AGTTAGAGCG AAAGAGGAAG 
GAGGCATATG AAAGAGAGAA AAGAGCTTGG GAAGAACATC TGATAGCAAA AGGAGTAAAG 
AATCCTAATG TGCCTTTTCA TGTGGGAGCA GCAGGACACA CTCCTGTGCA TGGAATACAA 
GAGCCTGGAG TGTCTCCTCC TAAGCAACAG CTTCCAGTTA AGCCCATCTC CATGACTTCT 
GCTTTGAAAG AAGTTGGCGT GGATGGAAAT GTAACCGCTG CCCAGGAGGT TAAAGAGGAA 
ATAAAAAAGC CAGATCTTAC AATGCAGAAC AAGAGGCAGA TACTGAAAAG ATTGAATCAA 
AATCTTAAAG CTCAGGAAGA TGAGAAAGGA AGAAAGGACG ATAAAAATAT CTCTGAGTCA 
GATGGGTCTG AAGATGGTAA AGAAGAGCAA GAAGATAACC ATCCACTTCT GGGAGATCGC 
AAAAGATGGG CATCAAGGGC ATCAGATTTG GTAGTTCCTT TAGCTCAGTT ATCAATGGAA 
GATTCTCTCT CTGGAACAGA CCGGCAAACT ATGGGGGAAG TAATCAGGCT AGATATTGGG 
GAACCCCGCA GGAAAGTCTG GGGCAAAAGC CCTACTGATT CGGTCCTGAA GATTCTAGGA 
GAAGCGGAGT TGCAGCTACC AACTGAATTA CTGGAAGAAA TAGATGTAAT AAATGAGCCT 
GATGATTTGG AAGCAGTGAT GTTGAAAGAA ACAGAAGAAG ATAAAAAGCA CCAGAGCAAG 
GAATTTCCCA TTGCTGTTAA TGAAGTGTGG GTGAAAGAAA AACAGGATGA GGCACAACAG 
GATGCAAGAA ATGAAGCAGC TTTTGAGAAA GAAGTAGTGC TTGATGAAGT GCAATCACAA 
AATGAAGCTC CAGAAGAAAC TTGTTCCAGT GACTCTCTGC AACCTGAACC GTTATTCCAG 
AGAGTAATAC AGCCCCCAGC TGTACCTACA GCCTCACCAG TTCTATCATC TCAGTCTTCC 
CAAGAAGAGC CTTTTGTACC TCGTTCACGT TCCATATCGC CAGCAAAAAA TAAAGGGAAA 
AGTTCATTGT TTTTTGGACT TTCTACAGGT CTGTTTGATG CAAACAACCC AAAGATGTTG 
AGAACGTGTT CACTCCCGGA TCTTTACAAA CTGTACAGAA CCTTAGTTGA TGTTCCCAGC 
ATAGCAGATG TACAGCATCA ACACAGTCTT GAAATAGATG ATACGGAAGA TGAGCAAGCC 
AAAGAAGGAC CCTCTGATTC TGAGGATATC ATGTTTGGGG ACGCCGATAC AGATTTGCAG 
GAACTCCGGG CCTCAATGGA ACAATTACTT AGGGAGCAGC CTAGTGAGGA ATTAAGTGAA 
GAGGAGGAAT CTACTTTAAA GGCTAACGTC ATCAAATGCA TGACAAATGG TACAGAGCTG 
GATGAAGCAG ATGAAAATAA CCCCAGCAGT GAAAGTGCAC TTAATGAAGA ATGGCAGTCA 
GATAATAGTG ATGGAGATAT TGCCAGTGAA TGTGAAGAAT GTGATAGTGT CTTCAGCCAT 
TTGGAAGAGC TGAGATACAA CCTGGAACAG GAAATAGGCT TTGAGAAATT AATTGAAGTT 
TATAATAAAA TAAAGGCCAT ACATGAAGAT GAAGATGAAA ATATTGATAT TTGTTCAAGC 
ATAGTTCAGA CTATTCTGGG AAATGAACAC AAACACTTAT ATGCCAAAGT ACTTCACCTG 
GTCATGGCGG ATGGAGCCTA CCAGGAAGAT AATGACGAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015140861.1
CDS	130..3831
Translation	MDKYIKVQKIGEGSFGKAFLVKDKENSQQYVIKEINISKMSNKEREESRREVAVLANMKHPNIVLYRESFEENGCLYIVMDYCEGGDLFKKINAQKGVLFSEDQILDWFVQICLALKHIHDRKILHRDIKSQNIFLTKDGTIQLGDFGIARVLNSTVELARTCIGTPYYLSPEICQNRPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIISGSFPPVSVHYSYDLRNLLSQLFKRNPRDRPSVNSILEKNFIAKRVEKFLTPQLIAEEFSHKVFHKFGPHAGPAKRPAQGHISASIAPVQKITKPAAKYGVPLAMKKCYDAPKKLHEKKSLIKLRQAFPTPKKKIIPGEERRKMFEEPSKKKRLELPEKEKRQRDQISLLKAEEMRRLEKERMERINRAREQGWRNVLGSGGSGDVKAPYYGGGGGVGPFPASFRGQYEHYHAIFDQMQKETFKVTKERQAKWRAEVQGQEAVERGIPPGVRPGVPKGPAGHHHLPDADAIRKKVKRLKEVSKQASANRQKGWLPAERAKQVEEFWQRKREAMQNKARAEGHMGTLQNVADIYGGRPMSARGRKSRNKEEEEYLARLRQIRLQNFNERQQIKAKLRGEKNEAPSSDGQESSEEAEMKRKKIEAQKAQANARAAVLKEQLERKRKEAYEREKRAWEEHLIAKGVKNPNVPFHVGAAGHTPVHGIQEPGVSPPKQQLPVKPISMTSALKEVGVDGNVTAAQEVKEEIKKPDLTMQNKRQILKRLNQNLKAQEDEKGRKDDKNISESDGSEDGKEEQEDNHPLLGDRKRWASRASDLVVPLAQLSMEDSLSGTDRQTMGEVIRLDIGEPRRKVWGKSPTDSVLKILGEAELQLPTELLEEIDVINEPDDLEAVMLKETEEDKKHQSKEFPIAVNEVWVKEKQDEAQQDARNEAAFEKEVVLDEVQSQNEAPEETCSSDSLQPEPLFQRVIQPPAVPTASPVLSSQSSQEEPFVPRSRSISPAKNKGKSSLFFGLSTGLFDANNPKMLRTCSLPDLYKLYRTLVDVPSIADVQHQHSLEIDDTEDEQAKEGPSDSEDIMFGDADTDLQELRASMEQLLREQPSEELSEEEESTLKANVIKCMTNGTELDEADENNPSSESALNEEWQSDNSDGDIASECEECDSVFSHLEELRYNLEQEIGFEKLIEVYNKIKAIHEDEDENIDICSSIVQTILGNEHKHLYAKVLHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_015285375.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus NIMA-related kinase 1 (NEK1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015285375.1

ORF Insert Sequence:

ATGGATAAAT ACATTAAAGT GCAAAAAATT GGAGAAGGAT CCTTTGGGAA AGCATTTCTT 
GTTAAAGATA AAGAAAATAG CCAGCAGTAT GTTATTAAAG AAATTAACAT ATCTAAGATG 
TCAAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TACTGGCCAA CATGAAACAT 
CCAAATATTG TCCTGTATAG GGAGTCGTTT GAAGAAAATG GTTGTCTCTA CATAGTGATG 
GATTACTGTG AAGGAGGAGA TCTGTTCAAA AAAATAAATG CCCAGAAAGG AGTCCTGTTC 
TCTGAGGATC AGATTCTTGA TTGGTTTGTA CAGATCTGTT TGGCACTGAA ACATATACAT 
GATAGAAAAA TCTTACATCG CGACATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA 
ACAATACAGC TGGGAGATTT TGGAATCGCG AGAGTTCTGA ACAGTACTGT GGAATTGGCT 
CGCACTTGCA TAGGAACACC ATACTATTTA TCACCTGAAA TATGCCAGAA CAGGCCTTAC 
AACAATAAAA GTGATATCTG GGCCCTTGGT TGCGTTCTCT ATGAAATGTG CACACTTAAG 
CATGCATTTG AAGCTGGTAA CATGAAGAAC TTGGTACTGA AGATAATCTC TGGATCTTTC 
CCTCCTGTTT CTGTGCATTA TTCCTATGAC CTACGTAATC TACTGTCACA GTTATTTAAA 
AGGAATCCTA GGGATAGACC ATCAGTGAAC TCCATATTGG AGAAAAATTT CATAGCCAAA 
CGGGTTGAAA AATTTCTCAC ACCGCAGCTT ATTGCAGAAG AATTCAGTCA CAAAGTGTTT 
CACAAGTTTG GACCACATGC TGGACCAGCT AAAAGACCAG CTCAAGGACA TATCTCGGCT 
TCAATTGCAC CAGTGCAGAA AATTACCAAA CCTGCTGCAA AATACGGAGT GCCTTTAGCG 
ATGAAGAAGT GTTATGACGC ACCCAAAAAG CTTCATGAGA AAAAGTCATT GATTAAACTT 
AGGCAGGCCT TTCCAACTCC AAAGAAGAAA ATTATTCCAG GAGAAGAAAG GAGGAAAATG 
TTTGAGGAAC CTTCAAAAAA GAAAAGGTTA GAATTGCCTG AAAAAGAAAA ACGCCAGAGA 
GATCAGATCA GTTTACTGAA GGCAGAGGAA ATGAGAAGAT TGGAAAAAGA AAGGATGGAA 
CGAATAAACA GAGCCAGGGA GCAAGGATGG AGGAATGTGC TTGGTTCAGG TGGAAGTGGT 
GATGTTAAGG CACCATATTA TGGCGGTGGA GGTGGTGTTG GTCCTTTTCC TGCCTCTTTC 
AGAGGACAGT ATGAGCACTA TCATGCTATT TTTGACCAGA TGCAAAAGGA AACCTTCAAA 
GTAACTAAAG AGAGGCAAGC CAAATGGAGG GCGGAGGTAC AAGGCCAAGA AGCTGTTGAG 
AGAGGAATTC CACCTGGAGT ACGTCCAGGA GTTCCTAAGG GGCCTGCAGG TCATCACCAT 
TTACCTGATG CTGATGCAAT TAGGAAAAAA GTGAAAAGGT TGAAGGAGGT GTCTAAACAA 
GCCAGTGCAA ACAGGCAGAA AGGATGGCTA CCTGCAGAAA GAGCAAAGCA AGTTGAAGAA 
TTCTGGCAAC GAAAGAGGGA AGCCATGCAA AACAAAGCTC GTGCTGAGGG ACACATGGGT 
ACACTGCAAA ACGTGGCAGA TATCTATGGA GGCAGGCCCA TGTCTGCAAG AGGAAGGAAA 
TCCAGAAACA AGGAAGAAGA GGAATATTTG GCAAGATTAA GACAGATACG ACTACAAAAC 
TTCAATGAGC GTCAACAGAT TAAAGCAAAA CTTCGTGGAG AGAAGAATGA AGCTCCCAGT 
TCTGATGGAC AAGAGTCAAG TGAGGAAGCA GAAATGAAAC GCAAAAAGAT AGAAGCACAG 
AAGGCCCAAG CAAATGCTCG AGCTGCAGTT CTGAAAGAAC AGTTAGAGCG AAAGAGGAAG 
GAGGCATATG AAAGAGAGAA AAGAGCTTGG GAAGAACATC TGATAGCAAA AGGAGTAAAG 
AATCCTAATG TGCCTTTTCA TGTGGGAGCA GCAGGACACA CTCCTGTGCA TGGAATACAA 
GAGCCTGGAG TGTCTCCTCC TAAGCAACAG CTTCCAGTTA AGCCCATCTC CATGACTTCT 
GCTTTGAAAG AAGTTGGCGT GGATGGAAAT GTAACCGCTG CCCAGGAGGT TAAAGAGGAA 
ATAAAAAAGC CAGATCTTAC AATGCAGAAC AAGAGGCAGA TACTGAAAAG ATTGAATCAA 
AATCTTAAAG CTCAGGAAGA TGAGAAAGGA AGAAAGGACG ATAAAAATAT CTCTGAGTCA 
GATGGGTCTG AAGATGGTAA AGAAGAGCAA GAAGATAACC ATCCACTTCT GGGAGATCGC 
AAAAGATGGG CATCAAGGGC ATCAGATTTG GTAGTTCCTT TAGCTCAGTT ATCAATGGAA 
GATTCTCTCT CTGGAACAGA CCGGCAAACT ATGGGGGAAG TAATCAGGCT AGATATTGGG 
GAACCCCGCA GGAAAGTCTG GGGCAAAAGC CCTACTGATT CGGTCCTGAA GATTCTAGGA 
GAAGCGGAGT TGCAGCTACC AACTGAATTA CTGGAAGAAA TAGATGTAAT AAATGAGCCT 
GATGATTTGG AAGCAGTGAT GTTGAAAGAA ACAGAAGAAG ATAAAAAGCA CCAGAGCAAG 
GAATTTCCCA TTGCTGTTAA TGAAGTGTGG GTGAAAGAAA AACAGGATGA GGCACAACAG 
GATGCAAGAA ATGAAGCAGC TTTTGAGAAA GAAGTAGTGC TTGATGAAGT GCAATCACAA 
AATGAAGCTC CAGAAGAAAC TTGTTCCAGT GACTCTCTGC AACCTGAACC GTTATTCCAG 
AGAGTAATAC AGCCCCCAGC TGTACCTACA GCCTCACCAG TTCTATCATC TCAGTCTTCC 
CAAGAAGAGC CTTTTGTACC TCGTTCACGT TCCATATCGC CAGCAAAAAA TAAAGGGAAA 
AGTTCATTGT TTTTTGGACT TTCTACAGGT CTGTTTGATG CAAACAACCC AAAGATGTTG 
AGAACGTGTT CACTCCCGGA TCTTTACAAA CTGTACAGAA CCTTAGTTGA TGTTCCCAGC 
ATAGCAGATG TACAGCATCA ACACAGTCTT GAAATAGATG ATACGGAAGA TGAGCAAGCC 
AAAGAAGGAC CCTCTGATTC TGAGGATATC ATGTTTGGGG ACGCCGATAC AGATTTGCAG 
GAACTCCGGG CCTCAATGGA ACAATTACTT AGGGAGCAGC CTAGTGAGGA ATTAAGTGAA 
GAGGAGGAAT CTACTTTAAA GGCTAACGTC ATCAAATGCA TGACAAATGG TACAGAGCTG 
GATGAAGCAG ATGAAAATAA CCCCAGCAGT GAAAGTGCAC TTAATGAAGA ATGGCAGTCA 
GATAATAGTG ATGGAGATAT TGCCAGTGAA TGTGAAGAAT GTGATAGTGT CTTCAGCCAT 
TTGGAAGAGC TGAGATACAA CCTGGAACAG GAAATAGGCT TTGAGAAATT AATTGAAGTT 
TATAATAAAA TAAAGGCCAT ACATGAAGAT GAAGATGAAA ATATTGATAT TTGTTCAAGC 
ATAGTTCAGA CTATTCTGGG AAATGAACAC AAACACTTAT ATGCCAAAGT ACTTCACCTG 
GTCATGGCGG ATGGAGCCTA CCAGGAAGAT AATGACGAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_015285376.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015140862.1 Gallus gallus NIMA-related kinase 1 (NEK1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OGa07767
Clone ID Related Accession (Same CDS sequence)	XM_015285374.2 , XM_015285375.1 , XM_015285376.1 , XM_420401.5 , XM_420401.6 , XM_015285374.1 , XM_015285376.2 , XM_015285375.2
Accession Version	XM_015285376.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3702bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	serine/threonine-protein kinase Nek1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455855.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAAAT ACATTAAAGT GCAAAAAATT GGAGAAGGAT CCTTTGGGAA AGCATTTCTT 
GTTAAAGATA AAGAAAATAG CCAGCAGTAT GTTATTAAAG AAATTAACAT ATCTAAGATG 
TCAAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TACTGGCCAA CATGAAACAT 
CCAAATATTG TCCTGTATAG GGAGTCGTTT GAAGAAAATG GTTGTCTCTA CATAGTGATG 
GATTACTGTG AAGGAGGAGA TCTGTTCAAA AAAATAAATG CCCAGAAAGG AGTCCTGTTC 
TCTGAGGATC AGATTCTTGA TTGGTTTGTA CAGATCTGTT TGGCACTGAA ACATATACAT 
GATAGAAAAA TCTTACATCG CGACATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA 
ACAATACAGC TGGGAGATTT TGGAATCGCG AGAGTTCTGA ACAGTACTGT GGAATTGGCT 
CGCACTTGCA TAGGAACACC ATACTATTTA TCACCTGAAA TATGCCAGAA CAGGCCTTAC 
AACAATAAAA GTGATATCTG GGCCCTTGGT TGCGTTCTCT ATGAAATGTG CACACTTAAG 
CATGCATTTG AAGCTGGTAA CATGAAGAAC TTGGTACTGA AGATAATCTC TGGATCTTTC 
CCTCCTGTTT CTGTGCATTA TTCCTATGAC CTACGTAATC TACTGTCACA GTTATTTAAA 
AGGAATCCTA GGGATAGACC ATCAGTGAAC TCCATATTGG AGAAAAATTT CATAGCCAAA 
CGGGTTGAAA AATTTCTCAC ACCGCAGCTT ATTGCAGAAG AATTCAGTCA CAAAGTGTTT 
CACAAGTTTG GACCACATGC TGGACCAGCT AAAAGACCAG CTCAAGGACA TATCTCGGCT 
TCAATTGCAC CAGTGCAGAA AATTACCAAA CCTGCTGCAA AATACGGAGT GCCTTTAGCG 
ATGAAGAAGT GTTATGACGC ACCCAAAAAG CTTCATGAGA AAAAGTCATT GATTAAACTT 
AGGCAGGCCT TTCCAACTCC AAAGAAGAAA ATTATTCCAG GAGAAGAAAG GAGGAAAATG 
TTTGAGGAAC CTTCAAAAAA GAAAAGGTTA GAATTGCCTG AAAAAGAAAA ACGCCAGAGA 
GATCAGATCA GTTTACTGAA GGCAGAGGAA ATGAGAAGAT TGGAAAAAGA AAGGATGGAA 
CGAATAAACA GAGCCAGGGA GCAAGGATGG AGGAATGTGC TTGGTTCAGG TGGAAGTGGT 
GATGTTAAGG CACCATATTA TGGCGGTGGA GGTGGTGTTG GTCCTTTTCC TGCCTCTTTC 
AGAGGACAGT ATGAGCACTA TCATGCTATT TTTGACCAGA TGCAAAAGGA AACCTTCAAA 
GTAACTAAAG AGAGGCAAGC CAAATGGAGG GCGGAGGTAC AAGGCCAAGA AGCTGTTGAG 
AGAGGAATTC CACCTGGAGT ACGTCCAGGA GTTCCTAAGG GGCCTGCAGG TCATCACCAT 
TTACCTGATG CTGATGCAAT TAGGAAAAAA GTGAAAAGGT TGAAGGAGGT GTCTAAACAA 
GCCAGTGCAA ACAGGCAGAA AGGATGGCTA CCTGCAGAAA GAGCAAAGCA AGTTGAAGAA 
TTCTGGCAAC GAAAGAGGGA AGCCATGCAA AACAAAGCTC GTGCTGAGGG ACACATGGGT 
ACACTGCAAA ACGTGGCAGA TATCTATGGA GGCAGGCCCA TGTCTGCAAG AGGAAGGAAA 
TCCAGAAACA AGGAAGAAGA GGAATATTTG GCAAGATTAA GACAGATACG ACTACAAAAC 
TTCAATGAGC GTCAACAGAT TAAAGCAAAA CTTCGTGGAG AGAAGAATGA AGCTCCCAGT 
TCTGATGGAC AAGAGTCAAG TGAGGAAGCA GAAATGAAAC GCAAAAAGAT AGAAGCACAG 
AAGGCCCAAG CAAATGCTCG AGCTGCAGTT CTGAAAGAAC AGTTAGAGCG AAAGAGGAAG 
GAGGCATATG AAAGAGAGAA AAGAGCTTGG GAAGAACATC TGATAGCAAA AGGAGTAAAG 
AATCCTAATG TGCCTTTTCA TGTGGGAGCA GCAGGACACA CTCCTGTGCA TGGAATACAA 
GAGCCTGGAG TGTCTCCTCC TAAGCAACAG CTTCCAGTTA AGCCCATCTC CATGACTTCT 
GCTTTGAAAG AAGTTGGCGT GGATGGAAAT GTAACCGCTG CCCAGGAGGT TAAAGAGGAA 
ATAAAAAAGC CAGATCTTAC AATGCAGAAC AAGAGGCAGA TACTGAAAAG ATTGAATCAA 
AATCTTAAAG CTCAGGAAGA TGAGAAAGGA AGAAAGGACG ATAAAAATAT CTCTGAGTCA 
GATGGGTCTG AAGATGGTAA AGAAGAGCAA GAAGATAACC ATCCACTTCT GGGAGATCGC 
AAAAGATGGG CATCAAGGGC ATCAGATTTG GTAGTTCCTT TAGCTCAGTT ATCAATGGAA 
GATTCTCTCT CTGGAACAGA CCGGCAAACT ATGGGGGAAG TAATCAGGCT AGATATTGGG 
GAACCCCGCA GGAAAGTCTG GGGCAAAAGC CCTACTGATT CGGTCCTGAA GATTCTAGGA 
GAAGCGGAGT TGCAGCTACC AACTGAATTA CTGGAAGAAA TAGATGTAAT AAATGAGCCT 
GATGATTTGG AAGCAGTGAT GTTGAAAGAA ACAGAAGAAG ATAAAAAGCA CCAGAGCAAG 
GAATTTCCCA TTGCTGTTAA TGAAGTGTGG GTGAAAGAAA AACAGGATGA GGCACAACAG 
GATGCAAGAA ATGAAGCAGC TTTTGAGAAA GAAGTAGTGC TTGATGAAGT GCAATCACAA 
AATGAAGCTC CAGAAGAAAC TTGTTCCAGT GACTCTCTGC AACCTGAACC GTTATTCCAG 
AGAGTAATAC AGCCCCCAGC TGTACCTACA GCCTCACCAG TTCTATCATC TCAGTCTTCC 
CAAGAAGAGC CTTTTGTACC TCGTTCACGT TCCATATCGC CAGCAAAAAA TAAAGGGAAA 
AGTTCATTGT TTTTTGGACT TTCTACAGGT CTGTTTGATG CAAACAACCC AAAGATGTTG 
AGAACGTGTT CACTCCCGGA TCTTTACAAA CTGTACAGAA CCTTAGTTGA TGTTCCCAGC 
ATAGCAGATG TACAGCATCA ACACAGTCTT GAAATAGATG ATACGGAAGA TGAGCAAGCC 
AAAGAAGGAC CCTCTGATTC TGAGGATATC ATGTTTGGGG ACGCCGATAC AGATTTGCAG 
GAACTCCGGG CCTCAATGGA ACAATTACTT AGGGAGCAGC CTAGTGAGGA ATTAAGTGAA 
GAGGAGGAAT CTACTTTAAA GGCTAACGTC ATCAAATGCA TGACAAATGG TACAGAGCTG 
GATGAAGCAG ATGAAAATAA CCCCAGCAGT GAAAGTGCAC TTAATGAAGA ATGGCAGTCA 
GATAATAGTG ATGGAGATAT TGCCAGTGAA TGTGAAGAAT GTGATAGTGT CTTCAGCCAT 
TTGGAAGAGC TGAGATACAA CCTGGAACAG GAAATAGGCT TTGAGAAATT AATTGAAGTT 
TATAATAAAA TAAAGGCCAT ACATGAAGAT GAAGATGAAA ATATTGATAT TTGTTCAAGC 
ATAGTTCAGA CTATTCTGGG AAATGAACAC AAACACTTAT ATGCCAAAGT ACTTCACCTG 
GTCATGGCGG ATGGAGCCTA CCAGGAAGAT AATGACGAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015140862.1
CDS	834..4535
Translation	MDKYIKVQKIGEGSFGKAFLVKDKENSQQYVIKEINISKMSNKEREESRREVAVLANMKHPNIVLYRESFEENGCLYIVMDYCEGGDLFKKINAQKGVLFSEDQILDWFVQICLALKHIHDRKILHRDIKSQNIFLTKDGTIQLGDFGIARVLNSTVELARTCIGTPYYLSPEICQNRPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIISGSFPPVSVHYSYDLRNLLSQLFKRNPRDRPSVNSILEKNFIAKRVEKFLTPQLIAEEFSHKVFHKFGPHAGPAKRPAQGHISASIAPVQKITKPAAKYGVPLAMKKCYDAPKKLHEKKSLIKLRQAFPTPKKKIIPGEERRKMFEEPSKKKRLELPEKEKRQRDQISLLKAEEMRRLEKERMERINRAREQGWRNVLGSGGSGDVKAPYYGGGGGVGPFPASFRGQYEHYHAIFDQMQKETFKVTKERQAKWRAEVQGQEAVERGIPPGVRPGVPKGPAGHHHLPDADAIRKKVKRLKEVSKQASANRQKGWLPAERAKQVEEFWQRKREAMQNKARAEGHMGTLQNVADIYGGRPMSARGRKSRNKEEEEYLARLRQIRLQNFNERQQIKAKLRGEKNEAPSSDGQESSEEAEMKRKKIEAQKAQANARAAVLKEQLERKRKEAYEREKRAWEEHLIAKGVKNPNVPFHVGAAGHTPVHGIQEPGVSPPKQQLPVKPISMTSALKEVGVDGNVTAAQEVKEEIKKPDLTMQNKRQILKRLNQNLKAQEDEKGRKDDKNISESDGSEDGKEEQEDNHPLLGDRKRWASRASDLVVPLAQLSMEDSLSGTDRQTMGEVIRLDIGEPRRKVWGKSPTDSVLKILGEAELQLPTELLEEIDVINEPDDLEAVMLKETEEDKKHQSKEFPIAVNEVWVKEKQDEAQQDARNEAAFEKEVVLDEVQSQNEAPEETCSSDSLQPEPLFQRVIQPPAVPTASPVLSSQSSQEEPFVPRSRSISPAKNKGKSSLFFGLSTGLFDANNPKMLRTCSLPDLYKLYRTLVDVPSIADVQHQHSLEIDDTEDEQAKEGPSDSEDIMFGDADTDLQELRASMEQLLREQPSEELSEEEESTLKANVIKCMTNGTELDEADENNPSSESALNEEWQSDNSDGDIASECEECDSVFSHLEELRYNLEQEIGFEKLIEVYNKIKAIHEDEDENIDICSSIVQTILGNEHKHLYAKVLHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_015285376.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus NIMA-related kinase 1 (NEK1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015285376.1

ORF Insert Sequence:

ATGGATAAAT ACATTAAAGT GCAAAAAATT GGAGAAGGAT CCTTTGGGAA AGCATTTCTT 
GTTAAAGATA AAGAAAATAG CCAGCAGTAT GTTATTAAAG AAATTAACAT ATCTAAGATG 
TCAAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TACTGGCCAA CATGAAACAT 
CCAAATATTG TCCTGTATAG GGAGTCGTTT GAAGAAAATG GTTGTCTCTA CATAGTGATG 
GATTACTGTG AAGGAGGAGA TCTGTTCAAA AAAATAAATG CCCAGAAAGG AGTCCTGTTC 
TCTGAGGATC AGATTCTTGA TTGGTTTGTA CAGATCTGTT TGGCACTGAA ACATATACAT 
GATAGAAAAA TCTTACATCG CGACATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA 
ACAATACAGC TGGGAGATTT TGGAATCGCG AGAGTTCTGA ACAGTACTGT GGAATTGGCT 
CGCACTTGCA TAGGAACACC ATACTATTTA TCACCTGAAA TATGCCAGAA CAGGCCTTAC 
AACAATAAAA GTGATATCTG GGCCCTTGGT TGCGTTCTCT ATGAAATGTG CACACTTAAG 
CATGCATTTG AAGCTGGTAA CATGAAGAAC TTGGTACTGA AGATAATCTC TGGATCTTTC 
CCTCCTGTTT CTGTGCATTA TTCCTATGAC CTACGTAATC TACTGTCACA GTTATTTAAA 
AGGAATCCTA GGGATAGACC ATCAGTGAAC TCCATATTGG AGAAAAATTT CATAGCCAAA 
CGGGTTGAAA AATTTCTCAC ACCGCAGCTT ATTGCAGAAG AATTCAGTCA CAAAGTGTTT 
CACAAGTTTG GACCACATGC TGGACCAGCT AAAAGACCAG CTCAAGGACA TATCTCGGCT 
TCAATTGCAC CAGTGCAGAA AATTACCAAA CCTGCTGCAA AATACGGAGT GCCTTTAGCG 
ATGAAGAAGT GTTATGACGC ACCCAAAAAG CTTCATGAGA AAAAGTCATT GATTAAACTT 
AGGCAGGCCT TTCCAACTCC AAAGAAGAAA ATTATTCCAG GAGAAGAAAG GAGGAAAATG 
TTTGAGGAAC CTTCAAAAAA GAAAAGGTTA GAATTGCCTG AAAAAGAAAA ACGCCAGAGA 
GATCAGATCA GTTTACTGAA GGCAGAGGAA ATGAGAAGAT TGGAAAAAGA AAGGATGGAA 
CGAATAAACA GAGCCAGGGA GCAAGGATGG AGGAATGTGC TTGGTTCAGG TGGAAGTGGT 
GATGTTAAGG CACCATATTA TGGCGGTGGA GGTGGTGTTG GTCCTTTTCC TGCCTCTTTC 
AGAGGACAGT ATGAGCACTA TCATGCTATT TTTGACCAGA TGCAAAAGGA AACCTTCAAA 
GTAACTAAAG AGAGGCAAGC CAAATGGAGG GCGGAGGTAC AAGGCCAAGA AGCTGTTGAG 
AGAGGAATTC CACCTGGAGT ACGTCCAGGA GTTCCTAAGG GGCCTGCAGG TCATCACCAT 
TTACCTGATG CTGATGCAAT TAGGAAAAAA GTGAAAAGGT TGAAGGAGGT GTCTAAACAA 
GCCAGTGCAA ACAGGCAGAA AGGATGGCTA CCTGCAGAAA GAGCAAAGCA AGTTGAAGAA 
TTCTGGCAAC GAAAGAGGGA AGCCATGCAA AACAAAGCTC GTGCTGAGGG ACACATGGGT 
ACACTGCAAA ACGTGGCAGA TATCTATGGA GGCAGGCCCA TGTCTGCAAG AGGAAGGAAA 
TCCAGAAACA AGGAAGAAGA GGAATATTTG GCAAGATTAA GACAGATACG ACTACAAAAC 
TTCAATGAGC GTCAACAGAT TAAAGCAAAA CTTCGTGGAG AGAAGAATGA AGCTCCCAGT 
TCTGATGGAC AAGAGTCAAG TGAGGAAGCA GAAATGAAAC GCAAAAAGAT AGAAGCACAG 
AAGGCCCAAG CAAATGCTCG AGCTGCAGTT CTGAAAGAAC AGTTAGAGCG AAAGAGGAAG 
GAGGCATATG AAAGAGAGAA AAGAGCTTGG GAAGAACATC TGATAGCAAA AGGAGTAAAG 
AATCCTAATG TGCCTTTTCA TGTGGGAGCA GCAGGACACA CTCCTGTGCA TGGAATACAA 
GAGCCTGGAG TGTCTCCTCC TAAGCAACAG CTTCCAGTTA AGCCCATCTC CATGACTTCT 
GCTTTGAAAG AAGTTGGCGT GGATGGAAAT GTAACCGCTG CCCAGGAGGT TAAAGAGGAA 
ATAAAAAAGC CAGATCTTAC AATGCAGAAC AAGAGGCAGA TACTGAAAAG ATTGAATCAA 
AATCTTAAAG CTCAGGAAGA TGAGAAAGGA AGAAAGGACG ATAAAAATAT CTCTGAGTCA 
GATGGGTCTG AAGATGGTAA AGAAGAGCAA GAAGATAACC ATCCACTTCT GGGAGATCGC 
AAAAGATGGG CATCAAGGGC ATCAGATTTG GTAGTTCCTT TAGCTCAGTT ATCAATGGAA 
GATTCTCTCT CTGGAACAGA CCGGCAAACT ATGGGGGAAG TAATCAGGCT AGATATTGGG 
GAACCCCGCA GGAAAGTCTG GGGCAAAAGC CCTACTGATT CGGTCCTGAA GATTCTAGGA 
GAAGCGGAGT TGCAGCTACC AACTGAATTA CTGGAAGAAA TAGATGTAAT AAATGAGCCT 
GATGATTTGG AAGCAGTGAT GTTGAAAGAA ACAGAAGAAG ATAAAAAGCA CCAGAGCAAG 
GAATTTCCCA TTGCTGTTAA TGAAGTGTGG GTGAAAGAAA AACAGGATGA GGCACAACAG 
GATGCAAGAA ATGAAGCAGC TTTTGAGAAA GAAGTAGTGC TTGATGAAGT GCAATCACAA 
AATGAAGCTC CAGAAGAAAC TTGTTCCAGT GACTCTCTGC AACCTGAACC GTTATTCCAG 
AGAGTAATAC AGCCCCCAGC TGTACCTACA GCCTCACCAG TTCTATCATC TCAGTCTTCC 
CAAGAAGAGC CTTTTGTACC TCGTTCACGT TCCATATCGC CAGCAAAAAA TAAAGGGAAA 
AGTTCATTGT TTTTTGGACT TTCTACAGGT CTGTTTGATG CAAACAACCC AAAGATGTTG 
AGAACGTGTT CACTCCCGGA TCTTTACAAA CTGTACAGAA CCTTAGTTGA TGTTCCCAGC 
ATAGCAGATG TACAGCATCA ACACAGTCTT GAAATAGATG ATACGGAAGA TGAGCAAGCC 
AAAGAAGGAC CCTCTGATTC TGAGGATATC ATGTTTGGGG ACGCCGATAC AGATTTGCAG 
GAACTCCGGG CCTCAATGGA ACAATTACTT AGGGAGCAGC CTAGTGAGGA ATTAAGTGAA 
GAGGAGGAAT CTACTTTAAA GGCTAACGTC ATCAAATGCA TGACAAATGG TACAGAGCTG 
GATGAAGCAG ATGAAAATAA CCCCAGCAGT GAAAGTGCAC TTAATGAAGA ATGGCAGTCA 
GATAATAGTG ATGGAGATAT TGCCAGTGAA TGTGAAGAAT GTGATAGTGT CTTCAGCCAT 
TTGGAAGAGC TGAGATACAA CCTGGAACAG GAAATAGGCT TTGAGAAATT AATTGAAGTT 
TATAATAAAA TAAAGGCCAT ACATGAAGAT GAAGATGAAA ATATTGATAT TTGTTCAAGC 
ATAGTTCAGA CTATTCTGGG AAATGAACAC AAACACTTAT ATGCCAAAGT ACTTCACCTG 
GTCATGGCGG ATGGAGCCTA CCAGGAAGAT AATGACGAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_420401.5 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_420401.3 Gallus gallus NIMA-related kinase 1 (NEK1), transcript variant X1, mRNA.

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CloneID	OGa07767
Clone ID Related Accession (Same CDS sequence)	XM_015285374.2 , XM_015285375.1 , XM_015285376.1 , XM_420401.5 , XM_420401.6 , XM_015285374.1 , XM_015285376.2 , XM_015285375.2
Accession Version	XM_420401.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3702bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	serine/threonine-protein kinase Nek1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455855.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_420401.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAAAT ACATTAAAGT GCAAAAAATT GGAGAAGGAT CCTTTGGGAA AGCATTTCTT 
GTTAAAGATA AAGAAAATAG CCAGCAGTAT GTTATTAAAG AAATTAACAT ATCTAAGATG 
TCAAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TACTGGCCAA CATGAAACAT 
CCAAATATTG TCCTGTATAG GGAGTCGTTT GAAGAAAATG GTTGTCTCTA CATAGTGATG 
GATTACTGTG AAGGAGGAGA TCTGTTCAAA AAAATAAATG CCCAGAAAGG AGTCCTGTTC 
TCTGAGGATC AGATTCTTGA TTGGTTTGTA CAGATCTGTT TGGCACTGAA ACATATACAT 
GATAGAAAAA TCTTACATCG CGACATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA 
ACAATACAGC TGGGAGATTT TGGAATCGCG AGAGTTCTGA ACAGTACTGT GGAATTGGCT 
CGCACTTGCA TAGGAACACC ATACTATTTA TCACCTGAAA TATGCCAGAA CAGGCCTTAC 
AACAATAAAA GTGATATCTG GGCCCTTGGT TGCGTTCTCT ATGAAATGTG CACACTTAAG 
CATGCATTTG AAGCTGGTAA CATGAAGAAC TTGGTACTGA AGATAATCTC TGGATCTTTC 
CCTCCTGTTT CTGTGCATTA TTCCTATGAC CTACGTAATC TACTGTCACA GTTATTTAAA 
AGGAATCCTA GGGATAGACC ATCAGTGAAC TCCATATTGG AGAAAAATTT CATAGCCAAA 
CGGGTTGAAA AATTTCTCAC ACCGCAGCTT ATTGCAGAAG AATTCAGTCA CAAAGTGTTT 
CACAAGTTTG GACCACATGC TGGACCAGCT AAAAGACCAG CTCAAGGACA TATCTCGGCT 
TCAATTGCAC CAGTGCAGAA AATTACCAAA CCTGCTGCAA AATACGGAGT GCCTTTAGCG 
ATGAAGAAGT GTTATGACGC ACCCAAAAAG CTTCATGAGA AAAAGTCATT GATTAAACTT 
AGGCAGGCCT TTCCAACTCC AAAGAAGAAA ATTATTCCAG GAGAAGAAAG GAGGAAAATG 
TTTGAGGAAC CTTCAAAAAA GAAAAGGTTA GAATTGCCTG AAAAAGAAAA ACGCCAGAGA 
GATCAGATCA GTTTACTGAA GGCAGAGGAA ATGAGAAGAT TGGAAAAAGA AAGGATGGAA 
CGAATAAACA GAGCCAGGGA GCAAGGATGG AGGAATGTGC TTGGTTCAGG TGGAAGTGGT 
GATGTTAAGG CACCATATTA TGGCGGTGGA GGTGGTGTTG GTCCTTTTCC TGCCTCTTTC 
AGAGGACAGT ATGAGCACTA TCATGCTATT TTTGACCAGA TGCAAAAGGA AACCTTCAAA 
GTAACTAAAG AGAGGCAAGC CAAATGGAGG GCGGAGGTAC AAGGCCAAGA AGCTGTTGAG 
AGAGGAATTC CACCTGGAGT ACGTCCAGGA GTTCCTAAGG GGCCTGCAGG TCATCACCAT 
TTACCTGATG CTGATGCAAT TAGGAAAAAA GTGAAAAGGT TGAAGGAGGT GTCTAAACAA 
GCCAGTGCAA ACAGGCAGAA AGGATGGCTA CCTGCAGAAA GAGCAAAGCA AGTTGAAGAA 
TTCTGGCAAC GAAAGAGGGA AGCCATGCAA AACAAAGCTC GTGCTGAGGG ACACATGGGT 
ACACTGCAAA ACGTGGCAGA TATCTATGGA GGCAGGCCCA TGTCTGCAAG AGGAAGGAAA 
TCCAGAAACA AGGAAGAAGA GGAATATTTG GCAAGATTAA GACAGATACG ACTACAAAAC 
TTCAATGAGC GTCAACAGAT TAAAGCAAAA CTTCGTGGAG AGAAGAATGA AGCTCCCAGT 
TCTGATGGAC AAGAGTCAAG TGAGGAAGCA GAAATGAAAC GCAAAAAGAT AGAAGCACAG 
AAGGCCCAAG CAAATGCTCG AGCTGCAGTT CTGAAAGAAC AGTTAGAGCG AAAGAGGAAG 
GAGGCATATG AAAGAGAGAA AAGAGCTTGG GAAGAACATC TGATAGCAAA AGGAGTAAAG 
AATCCTAATG TGCCTTTTCA TGTGGGAGCA GCAGGACACA CTCCTGTGCA TGGAATACAA 
GAGCCTGGAG TGTCTCCTCC TAAGCAACAG CTTCCAGTTA AGCCCATCTC CATGACTTCT 
GCTTTGAAAG AAGTTGGCGT GGATGGAAAT GTAACCGCTG CCCAGGAGGT TAAAGAGGAA 
ATAAAAAAGC CAGATCTTAC AATGCAGAAC AAGAGGCAGA TACTGAAAAG ATTGAATCAA 
AATCTTAAAG CTCAGGAAGA TGAGAAAGGA AGAAAGGACG ATAAAAATAT CTCTGAGTCA 
GATGGGTCTG AAGATGGTAA AGAAGAGCAA GAAGATAACC ATCCACTTCT GGGAGATCGC 
AAAAGATGGG CATCAAGGGC ATCAGATTTG GTAGTTCCTT TAGCTCAGTT ATCAATGGAA 
GATTCTCTCT CTGGAACAGA CCGGCAAACT ATGGGGGAAG TAATCAGGCT AGATATTGGG 
GAACCCCGCA GGAAAGTCTG GGGCAAAAGC CCTACTGATT CGGTCCTGAA GATTCTAGGA 
GAAGCGGAGT TGCAGCTACC AACTGAATTA CTGGAAGAAA TAGATGTAAT AAATGAGCCT 
GATGATTTGG AAGCAGTGAT GTTGAAAGAA ACAGAAGAAG ATAAAAAGCA CCAGAGCAAG 
GAATTTCCCA TTGCTGTTAA TGAAGTGTGG GTGAAAGAAA AACAGGATGA GGCACAACAG 
GATGCAAGAA ATGAAGCAGC TTTTGAGAAA GAAGTAGTGC TTGATGAAGT GCAATCACAA 
AATGAAGCTC CAGAAGAAAC TTGTTCCAGT GACTCTCTGC AACCTGAACC GTTATTCCAG 
AGAGTAATAC AGCCCCCAGC TGTACCTACA GCCTCACCAG TTCTATCATC TCAGTCTTCC 
CAAGAAGAGC CTTTTGTACC TCGTTCACGT TCCATATCGC CAGCAAAAAA TAAAGGGAAA 
AGTTCATTGT TTTTTGGACT TTCTACAGGT CTGTTTGATG CAAACAACCC AAAGATGTTG 
AGAACGTGTT CACTCCCGGA TCTTTACAAA CTGTACAGAA CCTTAGTTGA TGTTCCCAGC 
ATAGCAGATG TACAGCATCA ACACAGTCTT GAAATAGATG ATACGGAAGA TGAGCAAGCC 
AAAGAAGGAC CCTCTGATTC TGAGGATATC ATGTTTGGGG ACGCCGATAC AGATTTGCAG 
GAACTCCGGG CCTCAATGGA ACAATTACTT AGGGAGCAGC CTAGTGAGGA ATTAAGTGAA 
GAGGAGGAAT CTACTTTAAA GGCTAACGTC ATCAAATGCA TGACAAATGG TACAGAGCTG 
GATGAAGCAG ATGAAAATAA CCCCAGCAGT GAAAGTGCAC TTAATGAAGA ATGGCAGTCA 
GATAATAGTG ATGGAGATAT TGCCAGTGAA TGTGAAGAAT GTGATAGTGT CTTCAGCCAT 
TTGGAAGAGC TGAGATACAA CCTGGAACAG GAAATAGGCT TTGAGAAATT AATTGAAGTT 
TATAATAAAA TAAAGGCCAT ACATGAAGAT GAAGATGAAA ATATTGATAT TTGTTCAAGC 
ATAGTTCAGA CTATTCTGGG AAATGAACAC AAACACTTAT ATGCCAAAGT ACTTCACCTG 
GTCATGGCGG ATGGAGCCTA CCAGGAAGAT AATGACGAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_420401.3
CDS	182..3883
Translation	MDKYIKVQKIGEGSFGKAFLVKDKENSQQYVIKEINISKMSNKEREESRREVAVLANMKHPNIVLYRESFEENGCLYIVMDYCEGGDLFKKINAQKGVLFSEDQILDWFVQICLALKHIHDRKILHRDIKSQNIFLTKDGTIQLGDFGIARVLNSTVELARTCIGTPYYLSPEICQNRPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIISGSFPPVSVHYSYDLRNLLSQLFKRNPRDRPSVNSILEKNFIAKRVEKFLTPQLIAEEFSHKVFHKFGPHAGPAKRPAQGHISASIAPVQKITKPAAKYGVPLAMKKCYDAPKKLHEKKSLIKLRQAFPTPKKKIIPGEERRKMFEEPSKKKRLELPEKEKRQRDQISLLKAEEMRRLEKERMERINRAREQGWRNVLGSGGSGDVKAPYYGGGGGVGPFPASFRGQYEHYHAIFDQMQKETFKVTKERQAKWRAEVQGQEAVERGIPPGVRPGVPKGPAGHHHLPDADAIRKKVKRLKEVSKQASANRQKGWLPAERAKQVEEFWQRKREAMQNKARAEGHMGTLQNVADIYGGRPMSARGRKSRNKEEEEYLARLRQIRLQNFNERQQIKAKLRGEKNEAPSSDGQESSEEAEMKRKKIEAQKAQANARAAVLKEQLERKRKEAYEREKRAWEEHLIAKGVKNPNVPFHVGAAGHTPVHGIQEPGVSPPKQQLPVKPISMTSALKEVGVDGNVTAAQEVKEEIKKPDLTMQNKRQILKRLNQNLKAQEDEKGRKDDKNISESDGSEDGKEEQEDNHPLLGDRKRWASRASDLVVPLAQLSMEDSLSGTDRQTMGEVIRLDIGEPRRKVWGKSPTDSVLKILGEAELQLPTELLEEIDVINEPDDLEAVMLKETEEDKKHQSKEFPIAVNEVWVKEKQDEAQQDARNEAAFEKEVVLDEVQSQNEAPEETCSSDSLQPEPLFQRVIQPPAVPTASPVLSSQSSQEEPFVPRSRSISPAKNKGKSSLFFGLSTGLFDANNPKMLRTCSLPDLYKLYRTLVDVPSIADVQHQHSLEIDDTEDEQAKEGPSDSEDIMFGDADTDLQELRASMEQLLREQPSEELSEEEESTLKANVIKCMTNGTELDEADENNPSSESALNEEWQSDNSDGDIASECEECDSVFSHLEELRYNLEQEIGFEKLIEVYNKIKAIHEDEDENIDICSSIVQTILGNEHKHLYAKVLHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_420401.5
Organism	Gallus gallus(chicken)
Definition	Gallus gallus NIMA-related kinase 1 (NEK1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_420401.5

ORF Insert Sequence:

ATGGATAAAT ACATTAAAGT GCAAAAAATT GGAGAAGGAT CCTTTGGGAA AGCATTTCTT 
GTTAAAGATA AAGAAAATAG CCAGCAGTAT GTTATTAAAG AAATTAACAT ATCTAAGATG 
TCAAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TACTGGCCAA CATGAAACAT 
CCAAATATTG TCCTGTATAG GGAGTCGTTT GAAGAAAATG GTTGTCTCTA CATAGTGATG 
GATTACTGTG AAGGAGGAGA TCTGTTCAAA AAAATAAATG CCCAGAAAGG AGTCCTGTTC 
TCTGAGGATC AGATTCTTGA TTGGTTTGTA CAGATCTGTT TGGCACTGAA ACATATACAT 
GATAGAAAAA TCTTACATCG CGACATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA 
ACAATACAGC TGGGAGATTT TGGAATCGCG AGAGTTCTGA ACAGTACTGT GGAATTGGCT 
CGCACTTGCA TAGGAACACC ATACTATTTA TCACCTGAAA TATGCCAGAA CAGGCCTTAC 
AACAATAAAA GTGATATCTG GGCCCTTGGT TGCGTTCTCT ATGAAATGTG CACACTTAAG 
CATGCATTTG AAGCTGGTAA CATGAAGAAC TTGGTACTGA AGATAATCTC TGGATCTTTC 
CCTCCTGTTT CTGTGCATTA TTCCTATGAC CTACGTAATC TACTGTCACA GTTATTTAAA 
AGGAATCCTA GGGATAGACC ATCAGTGAAC TCCATATTGG AGAAAAATTT CATAGCCAAA 
CGGGTTGAAA AATTTCTCAC ACCGCAGCTT ATTGCAGAAG AATTCAGTCA CAAAGTGTTT 
CACAAGTTTG GACCACATGC TGGACCAGCT AAAAGACCAG CTCAAGGACA TATCTCGGCT 
TCAATTGCAC CAGTGCAGAA AATTACCAAA CCTGCTGCAA AATACGGAGT GCCTTTAGCG 
ATGAAGAAGT GTTATGACGC ACCCAAAAAG CTTCATGAGA AAAAGTCATT GATTAAACTT 
AGGCAGGCCT TTCCAACTCC AAAGAAGAAA ATTATTCCAG GAGAAGAAAG GAGGAAAATG 
TTTGAGGAAC CTTCAAAAAA GAAAAGGTTA GAATTGCCTG AAAAAGAAAA ACGCCAGAGA 
GATCAGATCA GTTTACTGAA GGCAGAGGAA ATGAGAAGAT TGGAAAAAGA AAGGATGGAA 
CGAATAAACA GAGCCAGGGA GCAAGGATGG AGGAATGTGC TTGGTTCAGG TGGAAGTGGT 
GATGTTAAGG CACCATATTA TGGCGGTGGA GGTGGTGTTG GTCCTTTTCC TGCCTCTTTC 
AGAGGACAGT ATGAGCACTA TCATGCTATT TTTGACCAGA TGCAAAAGGA AACCTTCAAA 
GTAACTAAAG AGAGGCAAGC CAAATGGAGG GCGGAGGTAC AAGGCCAAGA AGCTGTTGAG 
AGAGGAATTC CACCTGGAGT ACGTCCAGGA GTTCCTAAGG GGCCTGCAGG TCATCACCAT 
TTACCTGATG CTGATGCAAT TAGGAAAAAA GTGAAAAGGT TGAAGGAGGT GTCTAAACAA 
GCCAGTGCAA ACAGGCAGAA AGGATGGCTA CCTGCAGAAA GAGCAAAGCA AGTTGAAGAA 
TTCTGGCAAC GAAAGAGGGA AGCCATGCAA AACAAAGCTC GTGCTGAGGG ACACATGGGT 
ACACTGCAAA ACGTGGCAGA TATCTATGGA GGCAGGCCCA TGTCTGCAAG AGGAAGGAAA 
TCCAGAAACA AGGAAGAAGA GGAATATTTG GCAAGATTAA GACAGATACG ACTACAAAAC 
TTCAATGAGC GTCAACAGAT TAAAGCAAAA CTTCGTGGAG AGAAGAATGA AGCTCCCAGT 
TCTGATGGAC AAGAGTCAAG TGAGGAAGCA GAAATGAAAC GCAAAAAGAT AGAAGCACAG 
AAGGCCCAAG CAAATGCTCG AGCTGCAGTT CTGAAAGAAC AGTTAGAGCG AAAGAGGAAG 
GAGGCATATG AAAGAGAGAA AAGAGCTTGG GAAGAACATC TGATAGCAAA AGGAGTAAAG 
AATCCTAATG TGCCTTTTCA TGTGGGAGCA GCAGGACACA CTCCTGTGCA TGGAATACAA 
GAGCCTGGAG TGTCTCCTCC TAAGCAACAG CTTCCAGTTA AGCCCATCTC CATGACTTCT 
GCTTTGAAAG AAGTTGGCGT GGATGGAAAT GTAACCGCTG CCCAGGAGGT TAAAGAGGAA 
ATAAAAAAGC CAGATCTTAC AATGCAGAAC AAGAGGCAGA TACTGAAAAG ATTGAATCAA 
AATCTTAAAG CTCAGGAAGA TGAGAAAGGA AGAAAGGACG ATAAAAATAT CTCTGAGTCA 
GATGGGTCTG AAGATGGTAA AGAAGAGCAA GAAGATAACC ATCCACTTCT GGGAGATCGC 
AAAAGATGGG CATCAAGGGC ATCAGATTTG GTAGTTCCTT TAGCTCAGTT ATCAATGGAA 
GATTCTCTCT CTGGAACAGA CCGGCAAACT ATGGGGGAAG TAATCAGGCT AGATATTGGG 
GAACCCCGCA GGAAAGTCTG GGGCAAAAGC CCTACTGATT CGGTCCTGAA GATTCTAGGA 
GAAGCGGAGT TGCAGCTACC AACTGAATTA CTGGAAGAAA TAGATGTAAT AAATGAGCCT 
GATGATTTGG AAGCAGTGAT GTTGAAAGAA ACAGAAGAAG ATAAAAAGCA CCAGAGCAAG 
GAATTTCCCA TTGCTGTTAA TGAAGTGTGG GTGAAAGAAA AACAGGATGA GGCACAACAG 
GATGCAAGAA ATGAAGCAGC TTTTGAGAAA GAAGTAGTGC TTGATGAAGT GCAATCACAA 
AATGAAGCTC CAGAAGAAAC TTGTTCCAGT GACTCTCTGC AACCTGAACC GTTATTCCAG 
AGAGTAATAC AGCCCCCAGC TGTACCTACA GCCTCACCAG TTCTATCATC TCAGTCTTCC 
CAAGAAGAGC CTTTTGTACC TCGTTCACGT TCCATATCGC CAGCAAAAAA TAAAGGGAAA 
AGTTCATTGT TTTTTGGACT TTCTACAGGT CTGTTTGATG CAAACAACCC AAAGATGTTG 
AGAACGTGTT CACTCCCGGA TCTTTACAAA CTGTACAGAA CCTTAGTTGA TGTTCCCAGC 
ATAGCAGATG TACAGCATCA ACACAGTCTT GAAATAGATG ATACGGAAGA TGAGCAAGCC 
AAAGAAGGAC CCTCTGATTC TGAGGATATC ATGTTTGGGG ACGCCGATAC AGATTTGCAG 
GAACTCCGGG CCTCAATGGA ACAATTACTT AGGGAGCAGC CTAGTGAGGA ATTAAGTGAA 
GAGGAGGAAT CTACTTTAAA GGCTAACGTC ATCAAATGCA TGACAAATGG TACAGAGCTG 
GATGAAGCAG ATGAAAATAA CCCCAGCAGT GAAAGTGCAC TTAATGAAGA ATGGCAGTCA 
GATAATAGTG ATGGAGATAT TGCCAGTGAA TGTGAAGAAT GTGATAGTGT CTTCAGCCAT 
TTGGAAGAGC TGAGATACAA CCTGGAACAG GAAATAGGCT TTGAGAAATT AATTGAAGTT 
TATAATAAAA TAAAGGCCAT ACATGAAGAT GAAGATGAAA ATATTGATAT TTGTTCAAGC 
ATAGTTCAGA CTATTCTGGG AAATGAACAC AAACACTTAT ATGCCAAAGT ACTTCACCTG 
GTCATGGCGG ATGGAGCCTA CCAGGAAGAT AATGACGAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_420401.6 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_420401.3 Gallus gallus NIMA related kinase 1 (NEK1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa07767
Clone ID Related Accession (Same CDS sequence)	XM_015285374.2 , XM_015285375.1 , XM_015285376.1 , XM_420401.5 , XM_420401.6 , XM_015285374.1 , XM_015285376.2 , XM_015285375.2
Accession Version	XM_420401.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3702bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	serine/threonine-protein kinase Nek1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_420401.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAAAT ACATTAAAGT GCAAAAAATT GGAGAAGGAT CCTTTGGGAA AGCATTTCTT 
GTTAAAGATA AAGAAAATAG CCAGCAGTAT GTTATTAAAG AAATTAACAT ATCTAAGATG 
TCAAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TACTGGCCAA CATGAAACAT 
CCAAATATTG TCCTGTATAG GGAGTCGTTT GAAGAAAATG GTTGTCTCTA CATAGTGATG 
GATTACTGTG AAGGAGGAGA TCTGTTCAAA AAAATAAATG CCCAGAAAGG AGTCCTGTTC 
TCTGAGGATC AGATTCTTGA TTGGTTTGTA CAGATCTGTT TGGCACTGAA ACATATACAT 
GATAGAAAAA TCTTACATCG CGACATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA 
ACAATACAGC TGGGAGATTT TGGAATCGCG AGAGTTCTGA ACAGTACTGT GGAATTGGCT 
CGCACTTGCA TAGGAACACC ATACTATTTA TCACCTGAAA TATGCCAGAA CAGGCCTTAC 
AACAATAAAA GTGATATCTG GGCCCTTGGT TGCGTTCTCT ATGAAATGTG CACACTTAAG 
CATGCATTTG AAGCTGGTAA CATGAAGAAC TTGGTACTGA AGATAATCTC TGGATCTTTC 
CCTCCTGTTT CTGTGCATTA TTCCTATGAC CTACGTAATC TACTGTCACA GTTATTTAAA 
AGGAATCCTA GGGATAGACC ATCAGTGAAC TCCATATTGG AGAAAAATTT CATAGCCAAA 
CGGGTTGAAA AATTTCTCAC ACCGCAGCTT ATTGCAGAAG AATTCAGTCA CAAAGTGTTT 
CACAAGTTTG GACCACATGC TGGACCAGCT AAAAGACCAG CTCAAGGACA TATCTCGGCT 
TCAATTGCAC CAGTGCAGAA AATTACCAAA CCTGCTGCAA AATACGGAGT GCCTTTAGCG 
ATGAAGAAGT GTTATGACGC ACCCAAAAAG CTTCATGAGA AAAAGTCATT GATTAAACTT 
AGGCAGGCCT TTCCAACTCC AAAGAAGAAA ATTATTCCAG GAGAAGAAAG GAGGAAAATG 
TTTGAGGAAC CTTCAAAAAA GAAAAGGTTA GAATTGCCTG AAAAAGAAAA ACGCCAGAGA 
GATCAGATCA GTTTACTGAA GGCAGAGGAA ATGAGAAGAT TGGAAAAAGA AAGGATGGAA 
CGAATAAACA GAGCCAGGGA GCAAGGATGG AGGAATGTGC TTGGTTCAGG TGGAAGTGGT 
GATGTTAAGG CACCATATTA TGGCGGTGGA GGTGGTGTTG GTCCTTTTCC TGCCTCTTTC 
AGAGGACAGT ATGAGCACTA TCATGCTATT TTTGACCAGA TGCAAAAGGA AACCTTCAAA 
GTAACTAAAG AGAGGCAAGC CAAATGGAGG GCGGAGGTAC AAGGCCAAGA AGCTGTTGAG 
AGAGGAATTC CACCTGGAGT ACGTCCAGGA GTTCCTAAGG GGCCTGCAGG TCATCACCAT 
TTACCTGATG CTGATGCAAT TAGGAAAAAA GTGAAAAGGT TGAAGGAGGT GTCTAAACAA 
GCCAGTGCAA ACAGGCAGAA AGGATGGCTA CCTGCAGAAA GAGCAAAGCA AGTTGAAGAA 
TTCTGGCAAC GAAAGAGGGA AGCCATGCAA AACAAAGCTC GTGCTGAGGG ACACATGGGT 
ACACTGCAAA ACGTGGCAGA TATCTATGGA GGCAGGCCCA TGTCTGCAAG AGGAAGGAAA 
TCCAGAAACA AGGAAGAAGA GGAATATTTG GCAAGATTAA GACAGATACG ACTACAAAAC 
TTCAATGAGC GTCAACAGAT TAAAGCAAAA CTTCGTGGAG AGAAGAATGA AGCTCCCAGT 
TCTGATGGAC AAGAGTCAAG TGAGGAAGCA GAAATGAAAC GCAAAAAGAT AGAAGCACAG 
AAGGCCCAAG CAAATGCTCG AGCTGCAGTT CTGAAAGAAC AGTTAGAGCG AAAGAGGAAG 
GAGGCATATG AAAGAGAGAA AAGAGCTTGG GAAGAACATC TGATAGCAAA AGGAGTAAAG 
AATCCTAATG TGCCTTTTCA TGTGGGAGCA GCAGGACACA CTCCTGTGCA TGGAATACAA 
GAGCCTGGAG TGTCTCCTCC TAAGCAACAG CTTCCAGTTA AGCCCATCTC CATGACTTCT 
GCTTTGAAAG AAGTTGGCGT GGATGGAAAT GTAACCGCTG CCCAGGAGGT TAAAGAGGAA 
ATAAAAAAGC CAGATCTTAC AATGCAGAAC AAGAGGCAGA TACTGAAAAG ATTGAATCAA 
AATCTTAAAG CTCAGGAAGA TGAGAAAGGA AGAAAGGACG ATAAAAATAT CTCTGAGTCA 
GATGGGTCTG AAGATGGTAA AGAAGAGCAA GAAGATAACC ATCCACTTCT GGGAGATCGC 
AAAAGATGGG CATCAAGGGC ATCAGATTTG GTAGTTCCTT TAGCTCAGTT ATCAATGGAA 
GATTCTCTCT CTGGAACAGA CCGGCAAACT ATGGGGGAAG TAATCAGGCT AGATATTGGG 
GAACCCCGCA GGAAAGTCTG GGGCAAAAGC CCTACTGATT CGGTCCTGAA GATTCTAGGA 
GAAGCGGAGT TGCAGCTACC AACTGAATTA CTGGAAGAAA TAGATGTAAT AAATGAGCCT 
GATGATTTGG AAGCAGTGAT GTTGAAAGAA ACAGAAGAAG ATAAAAAGCA CCAGAGCAAG 
GAATTTCCCA TTGCTGTTAA TGAAGTGTGG GTGAAAGAAA AACAGGATGA GGCACAACAG 
GATGCAAGAA ATGAAGCAGC TTTTGAGAAA GAAGTAGTGC TTGATGAAGT GCAATCACAA 
AATGAAGCTC CAGAAGAAAC TTGTTCCAGT GACTCTCTGC AACCTGAACC GTTATTCCAG 
AGAGTAATAC AGCCCCCAGC TGTACCTACA GCCTCACCAG TTCTATCATC TCAGTCTTCC 
CAAGAAGAGC CTTTTGTACC TCGTTCACGT TCCATATCGC CAGCAAAAAA TAAAGGGAAA 
AGTTCATTGT TTTTTGGACT TTCTACAGGT CTGTTTGATG CAAACAACCC AAAGATGTTG 
AGAACGTGTT CACTCCCGGA TCTTTACAAA CTGTACAGAA CCTTAGTTGA TGTTCCCAGC 
ATAGCAGATG TACAGCATCA ACACAGTCTT GAAATAGATG ATACGGAAGA TGAGCAAGCC 
AAAGAAGGAC CCTCTGATTC TGAGGATATC ATGTTTGGGG ACGCCGATAC AGATTTGCAG 
GAACTCCGGG CCTCAATGGA ACAATTACTT AGGGAGCAGC CTAGTGAGGA ATTAAGTGAA 
GAGGAGGAAT CTACTTTAAA GGCTAACGTC ATCAAATGCA TGACAAATGG TACAGAGCTG 
GATGAAGCAG ATGAAAATAA CCCCAGCAGT GAAAGTGCAC TTAATGAAGA ATGGCAGTCA 
GATAATAGTG ATGGAGATAT TGCCAGTGAA TGTGAAGAAT GTGATAGTGT CTTCAGCCAT 
TTGGAAGAGC TGAGATACAA CCTGGAACAG GAAATAGGCT TTGAGAAATT AATTGAAGTT 
TATAATAAAA TAAAGGCCAT ACATGAAGAT GAAGATGAAA ATATTGATAT TTGTTCAAGC 
ATAGTTCAGA CTATTCTGGG AAATGAACAC AAACACTTAT ATGCCAAAGT ACTTCACCTG 
GTCATGGCGG ATGGAGCCTA CCAGGAAGAT AATGACGAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_420401.3
CDS	226..3927
Translation	MDKYIKVQKIGEGSFGKAFLVKDKENSQQYVIKEINISKMSNKEREESRREVAVLANMKHPNIVLYRESFEENGCLYIVMDYCEGGDLFKKINAQKGVLFSEDQILDWFVQICLALKHIHDRKILHRDIKSQNIFLTKDGTIQLGDFGIARVLNSTVELARTCIGTPYYLSPEICQNRPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIISGSFPPVSVHYSYDLRNLLSQLFKRNPRDRPSVNSILEKNFIAKRVEKFLTPQLIAEEFSHKVFHKFGPHAGPAKRPAQGHISASIAPVQKITKPAAKYGVPLAMKKCYDAPKKLHEKKSLIKLRQAFPTPKKKIIPGEERRKMFEEPSKKKRLELPEKEKRQRDQISLLKAEEMRRLEKERMERINRAREQGWRNVLGSGGSGDVKAPYYGGGGGVGPFPASFRGQYEHYHAIFDQMQKETFKVTKERQAKWRAEVQGQEAVERGIPPGVRPGVPKGPAGHHHLPDADAIRKKVKRLKEVSKQASANRQKGWLPAERAKQVEEFWQRKREAMQNKARAEGHMGTLQNVADIYGGRPMSARGRKSRNKEEEEYLARLRQIRLQNFNERQQIKAKLRGEKNEAPSSDGQESSEEAEMKRKKIEAQKAQANARAAVLKEQLERKRKEAYEREKRAWEEHLIAKGVKNPNVPFHVGAAGHTPVHGIQEPGVSPPKQQLPVKPISMTSALKEVGVDGNVTAAQEVKEEIKKPDLTMQNKRQILKRLNQNLKAQEDEKGRKDDKNISESDGSEDGKEEQEDNHPLLGDRKRWASRASDLVVPLAQLSMEDSLSGTDRQTMGEVIRLDIGEPRRKVWGKSPTDSVLKILGEAELQLPTELLEEIDVINEPDDLEAVMLKETEEDKKHQSKEFPIAVNEVWVKEKQDEAQQDARNEAAFEKEVVLDEVQSQNEAPEETCSSDSLQPEPLFQRVIQPPAVPTASPVLSSQSSQEEPFVPRSRSISPAKNKGKSSLFFGLSTGLFDANNPKMLRTCSLPDLYKLYRTLVDVPSIADVQHQHSLEIDDTEDEQAKEGPSDSEDIMFGDADTDLQELRASMEQLLREQPSEELSEEEESTLKANVIKCMTNGTELDEADENNPSSESALNEEWQSDNSDGDIASECEECDSVFSHLEELRYNLEQEIGFEKLIEVYNKIKAIHEDEDENIDICSSIVQTILGNEHKHLYAKVLHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_420401.6
Organism	Gallus gallus(chicken)
Definition	Gallus gallus NIMA related kinase 1 (NEK1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_420401.6

ORF Insert Sequence:

ATGGATAAAT ACATTAAAGT GCAAAAAATT GGAGAAGGAT CCTTTGGGAA AGCATTTCTT 
GTTAAAGATA AAGAAAATAG CCAGCAGTAT GTTATTAAAG AAATTAACAT ATCTAAGATG 
TCAAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TACTGGCCAA CATGAAACAT 
CCAAATATTG TCCTGTATAG GGAGTCGTTT GAAGAAAATG GTTGTCTCTA CATAGTGATG 
GATTACTGTG AAGGAGGAGA TCTGTTCAAA AAAATAAATG CCCAGAAAGG AGTCCTGTTC 
TCTGAGGATC AGATTCTTGA TTGGTTTGTA CAGATCTGTT TGGCACTGAA ACATATACAT 
GATAGAAAAA TCTTACATCG CGACATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA 
ACAATACAGC TGGGAGATTT TGGAATCGCG AGAGTTCTGA ACAGTACTGT GGAATTGGCT 
CGCACTTGCA TAGGAACACC ATACTATTTA TCACCTGAAA TATGCCAGAA CAGGCCTTAC 
AACAATAAAA GTGATATCTG GGCCCTTGGT TGCGTTCTCT ATGAAATGTG CACACTTAAG 
CATGCATTTG AAGCTGGTAA CATGAAGAAC TTGGTACTGA AGATAATCTC TGGATCTTTC 
CCTCCTGTTT CTGTGCATTA TTCCTATGAC CTACGTAATC TACTGTCACA GTTATTTAAA 
AGGAATCCTA GGGATAGACC ATCAGTGAAC TCCATATTGG AGAAAAATTT CATAGCCAAA 
CGGGTTGAAA AATTTCTCAC ACCGCAGCTT ATTGCAGAAG AATTCAGTCA CAAAGTGTTT 
CACAAGTTTG GACCACATGC TGGACCAGCT AAAAGACCAG CTCAAGGACA TATCTCGGCT 
TCAATTGCAC CAGTGCAGAA AATTACCAAA CCTGCTGCAA AATACGGAGT GCCTTTAGCG 
ATGAAGAAGT GTTATGACGC ACCCAAAAAG CTTCATGAGA AAAAGTCATT GATTAAACTT 
AGGCAGGCCT TTCCAACTCC AAAGAAGAAA ATTATTCCAG GAGAAGAAAG GAGGAAAATG 
TTTGAGGAAC CTTCAAAAAA GAAAAGGTTA GAATTGCCTG AAAAAGAAAA ACGCCAGAGA 
GATCAGATCA GTTTACTGAA GGCAGAGGAA ATGAGAAGAT TGGAAAAAGA AAGGATGGAA 
CGAATAAACA GAGCCAGGGA GCAAGGATGG AGGAATGTGC TTGGTTCAGG TGGAAGTGGT 
GATGTTAAGG CACCATATTA TGGCGGTGGA GGTGGTGTTG GTCCTTTTCC TGCCTCTTTC 
AGAGGACAGT ATGAGCACTA TCATGCTATT TTTGACCAGA TGCAAAAGGA AACCTTCAAA 
GTAACTAAAG AGAGGCAAGC CAAATGGAGG GCGGAGGTAC AAGGCCAAGA AGCTGTTGAG 
AGAGGAATTC CACCTGGAGT ACGTCCAGGA GTTCCTAAGG GGCCTGCAGG TCATCACCAT 
TTACCTGATG CTGATGCAAT TAGGAAAAAA GTGAAAAGGT TGAAGGAGGT GTCTAAACAA 
GCCAGTGCAA ACAGGCAGAA AGGATGGCTA CCTGCAGAAA GAGCAAAGCA AGTTGAAGAA 
TTCTGGCAAC GAAAGAGGGA AGCCATGCAA AACAAAGCTC GTGCTGAGGG ACACATGGGT 
ACACTGCAAA ACGTGGCAGA TATCTATGGA GGCAGGCCCA TGTCTGCAAG AGGAAGGAAA 
TCCAGAAACA AGGAAGAAGA GGAATATTTG GCAAGATTAA GACAGATACG ACTACAAAAC 
TTCAATGAGC GTCAACAGAT TAAAGCAAAA CTTCGTGGAG AGAAGAATGA AGCTCCCAGT 
TCTGATGGAC AAGAGTCAAG TGAGGAAGCA GAAATGAAAC GCAAAAAGAT AGAAGCACAG 
AAGGCCCAAG CAAATGCTCG AGCTGCAGTT CTGAAAGAAC AGTTAGAGCG AAAGAGGAAG 
GAGGCATATG AAAGAGAGAA AAGAGCTTGG GAAGAACATC TGATAGCAAA AGGAGTAAAG 
AATCCTAATG TGCCTTTTCA TGTGGGAGCA GCAGGACACA CTCCTGTGCA TGGAATACAA 
GAGCCTGGAG TGTCTCCTCC TAAGCAACAG CTTCCAGTTA AGCCCATCTC CATGACTTCT 
GCTTTGAAAG AAGTTGGCGT GGATGGAAAT GTAACCGCTG CCCAGGAGGT TAAAGAGGAA 
ATAAAAAAGC CAGATCTTAC AATGCAGAAC AAGAGGCAGA TACTGAAAAG ATTGAATCAA 
AATCTTAAAG CTCAGGAAGA TGAGAAAGGA AGAAAGGACG ATAAAAATAT CTCTGAGTCA 
GATGGGTCTG AAGATGGTAA AGAAGAGCAA GAAGATAACC ATCCACTTCT GGGAGATCGC 
AAAAGATGGG CATCAAGGGC ATCAGATTTG GTAGTTCCTT TAGCTCAGTT ATCAATGGAA 
GATTCTCTCT CTGGAACAGA CCGGCAAACT ATGGGGGAAG TAATCAGGCT AGATATTGGG 
GAACCCCGCA GGAAAGTCTG GGGCAAAAGC CCTACTGATT CGGTCCTGAA GATTCTAGGA 
GAAGCGGAGT TGCAGCTACC AACTGAATTA CTGGAAGAAA TAGATGTAAT AAATGAGCCT 
GATGATTTGG AAGCAGTGAT GTTGAAAGAA ACAGAAGAAG ATAAAAAGCA CCAGAGCAAG 
GAATTTCCCA TTGCTGTTAA TGAAGTGTGG GTGAAAGAAA AACAGGATGA GGCACAACAG 
GATGCAAGAA ATGAAGCAGC TTTTGAGAAA GAAGTAGTGC TTGATGAAGT GCAATCACAA 
AATGAAGCTC CAGAAGAAAC TTGTTCCAGT GACTCTCTGC AACCTGAACC GTTATTCCAG 
AGAGTAATAC AGCCCCCAGC TGTACCTACA GCCTCACCAG TTCTATCATC TCAGTCTTCC 
CAAGAAGAGC CTTTTGTACC TCGTTCACGT TCCATATCGC CAGCAAAAAA TAAAGGGAAA 
AGTTCATTGT TTTTTGGACT TTCTACAGGT CTGTTTGATG CAAACAACCC AAAGATGTTG 
AGAACGTGTT CACTCCCGGA TCTTTACAAA CTGTACAGAA CCTTAGTTGA TGTTCCCAGC 
ATAGCAGATG TACAGCATCA ACACAGTCTT GAAATAGATG ATACGGAAGA TGAGCAAGCC 
AAAGAAGGAC CCTCTGATTC TGAGGATATC ATGTTTGGGG ACGCCGATAC AGATTTGCAG 
GAACTCCGGG CCTCAATGGA ACAATTACTT AGGGAGCAGC CTAGTGAGGA ATTAAGTGAA 
GAGGAGGAAT CTACTTTAAA GGCTAACGTC ATCAAATGCA TGACAAATGG TACAGAGCTG 
GATGAAGCAG ATGAAAATAA CCCCAGCAGT GAAAGTGCAC TTAATGAAGA ATGGCAGTCA 
GATAATAGTG ATGGAGATAT TGCCAGTGAA TGTGAAGAAT GTGATAGTGT CTTCAGCCAT 
TTGGAAGAGC TGAGATACAA CCTGGAACAG GAAATAGGCT TTGAGAAATT AATTGAAGTT 
TATAATAAAA TAAAGGCCAT ACATGAAGAT GAAGATGAAA ATATTGATAT TTGTTCAAGC 
ATAGTTCAGA CTATTCTGGG AAATGAACAC AAACACTTAT ATGCCAAAGT ACTTCACCTG 
GTCATGGCGG ATGGAGCCTA CCAGGAAGAT AATGACGAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_015285374.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015140860.1 Gallus gallus NIMA-related kinase 1 (NEK1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OGa07767
Clone ID Related Accession (Same CDS sequence)	XM_015285374.2 , XM_015285375.1 , XM_015285376.1 , XM_420401.5 , XM_420401.6 , XM_015285374.1 , XM_015285376.2 , XM_015285375.2
Accession Version	XM_015285374.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3702bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	serine/threonine-protein kinase Nek1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455855.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAAAT ACATTAAAGT GCAAAAAATT GGAGAAGGAT CCTTTGGGAA AGCATTTCTT 
GTTAAAGATA AAGAAAATAG CCAGCAGTAT GTTATTAAAG AAATTAACAT ATCTAAGATG 
TCAAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TACTGGCCAA CATGAAACAT 
CCAAATATTG TCCTGTATAG GGAGTCGTTT GAAGAAAATG GTTGTCTCTA CATAGTGATG 
GATTACTGTG AAGGAGGAGA TCTGTTCAAA AAAATAAATG CCCAGAAAGG AGTCCTGTTC 
TCTGAGGATC AGATTCTTGA TTGGTTTGTA CAGATCTGTT TGGCACTGAA ACATATACAT 
GATAGAAAAA TCTTACATCG CGACATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA 
ACAATACAGC TGGGAGATTT TGGAATCGCG AGAGTTCTGA ACAGTACTGT GGAATTGGCT 
CGCACTTGCA TAGGAACACC ATACTATTTA TCACCTGAAA TATGCCAGAA CAGGCCTTAC 
AACAATAAAA GTGATATCTG GGCCCTTGGT TGCGTTCTCT ATGAAATGTG CACACTTAAG 
CATGCATTTG AAGCTGGTAA CATGAAGAAC TTGGTACTGA AGATAATCTC TGGATCTTTC 
CCTCCTGTTT CTGTGCATTA TTCCTATGAC CTACGTAATC TACTGTCACA GTTATTTAAA 
AGGAATCCTA GGGATAGACC ATCAGTGAAC TCCATATTGG AGAAAAATTT CATAGCCAAA 
CGGGTTGAAA AATTTCTCAC ACCGCAGCTT ATTGCAGAAG AATTCAGTCA CAAAGTGTTT 
CACAAGTTTG GACCACATGC TGGACCAGCT AAAAGACCAG CTCAAGGACA TATCTCGGCT 
TCAATTGCAC CAGTGCAGAA AATTACCAAA CCTGCTGCAA AATACGGAGT GCCTTTAGCG 
ATGAAGAAGT GTTATGACGC ACCCAAAAAG CTTCATGAGA AAAAGTCATT GATTAAACTT 
AGGCAGGCCT TTCCAACTCC AAAGAAGAAA ATTATTCCAG GAGAAGAAAG GAGGAAAATG 
TTTGAGGAAC CTTCAAAAAA GAAAAGGTTA GAATTGCCTG AAAAAGAAAA ACGCCAGAGA 
GATCAGATCA GTTTACTGAA GGCAGAGGAA ATGAGAAGAT TGGAAAAAGA AAGGATGGAA 
CGAATAAACA GAGCCAGGGA GCAAGGATGG AGGAATGTGC TTGGTTCAGG TGGAAGTGGT 
GATGTTAAGG CACCATATTA TGGCGGTGGA GGTGGTGTTG GTCCTTTTCC TGCCTCTTTC 
AGAGGACAGT ATGAGCACTA TCATGCTATT TTTGACCAGA TGCAAAAGGA AACCTTCAAA 
GTAACTAAAG AGAGGCAAGC CAAATGGAGG GCGGAGGTAC AAGGCCAAGA AGCTGTTGAG 
AGAGGAATTC CACCTGGAGT ACGTCCAGGA GTTCCTAAGG GGCCTGCAGG TCATCACCAT 
TTACCTGATG CTGATGCAAT TAGGAAAAAA GTGAAAAGGT TGAAGGAGGT GTCTAAACAA 
GCCAGTGCAA ACAGGCAGAA AGGATGGCTA CCTGCAGAAA GAGCAAAGCA AGTTGAAGAA 
TTCTGGCAAC GAAAGAGGGA AGCCATGCAA AACAAAGCTC GTGCTGAGGG ACACATGGGT 
ACACTGCAAA ACGTGGCAGA TATCTATGGA GGCAGGCCCA TGTCTGCAAG AGGAAGGAAA 
TCCAGAAACA AGGAAGAAGA GGAATATTTG GCAAGATTAA GACAGATACG ACTACAAAAC 
TTCAATGAGC GTCAACAGAT TAAAGCAAAA CTTCGTGGAG AGAAGAATGA AGCTCCCAGT 
TCTGATGGAC AAGAGTCAAG TGAGGAAGCA GAAATGAAAC GCAAAAAGAT AGAAGCACAG 
AAGGCCCAAG CAAATGCTCG AGCTGCAGTT CTGAAAGAAC AGTTAGAGCG AAAGAGGAAG 
GAGGCATATG AAAGAGAGAA AAGAGCTTGG GAAGAACATC TGATAGCAAA AGGAGTAAAG 
AATCCTAATG TGCCTTTTCA TGTGGGAGCA GCAGGACACA CTCCTGTGCA TGGAATACAA 
GAGCCTGGAG TGTCTCCTCC TAAGCAACAG CTTCCAGTTA AGCCCATCTC CATGACTTCT 
GCTTTGAAAG AAGTTGGCGT GGATGGAAAT GTAACCGCTG CCCAGGAGGT TAAAGAGGAA 
ATAAAAAAGC CAGATCTTAC AATGCAGAAC AAGAGGCAGA TACTGAAAAG ATTGAATCAA 
AATCTTAAAG CTCAGGAAGA TGAGAAAGGA AGAAAGGACG ATAAAAATAT CTCTGAGTCA 
GATGGGTCTG AAGATGGTAA AGAAGAGCAA GAAGATAACC ATCCACTTCT GGGAGATCGC 
AAAAGATGGG CATCAAGGGC ATCAGATTTG GTAGTTCCTT TAGCTCAGTT ATCAATGGAA 
GATTCTCTCT CTGGAACAGA CCGGCAAACT ATGGGGGAAG TAATCAGGCT AGATATTGGG 
GAACCCCGCA GGAAAGTCTG GGGCAAAAGC CCTACTGATT CGGTCCTGAA GATTCTAGGA 
GAAGCGGAGT TGCAGCTACC AACTGAATTA CTGGAAGAAA TAGATGTAAT AAATGAGCCT 
GATGATTTGG AAGCAGTGAT GTTGAAAGAA ACAGAAGAAG ATAAAAAGCA CCAGAGCAAG 
GAATTTCCCA TTGCTGTTAA TGAAGTGTGG GTGAAAGAAA AACAGGATGA GGCACAACAG 
GATGCAAGAA ATGAAGCAGC TTTTGAGAAA GAAGTAGTGC TTGATGAAGT GCAATCACAA 
AATGAAGCTC CAGAAGAAAC TTGTTCCAGT GACTCTCTGC AACCTGAACC GTTATTCCAG 
AGAGTAATAC AGCCCCCAGC TGTACCTACA GCCTCACCAG TTCTATCATC TCAGTCTTCC 
CAAGAAGAGC CTTTTGTACC TCGTTCACGT TCCATATCGC CAGCAAAAAA TAAAGGGAAA 
AGTTCATTGT TTTTTGGACT TTCTACAGGT CTGTTTGATG CAAACAACCC AAAGATGTTG 
AGAACGTGTT CACTCCCGGA TCTTTACAAA CTGTACAGAA CCTTAGTTGA TGTTCCCAGC 
ATAGCAGATG TACAGCATCA ACACAGTCTT GAAATAGATG ATACGGAAGA TGAGCAAGCC 
AAAGAAGGAC CCTCTGATTC TGAGGATATC ATGTTTGGGG ACGCCGATAC AGATTTGCAG 
GAACTCCGGG CCTCAATGGA ACAATTACTT AGGGAGCAGC CTAGTGAGGA ATTAAGTGAA 
GAGGAGGAAT CTACTTTAAA GGCTAACGTC ATCAAATGCA TGACAAATGG TACAGAGCTG 
GATGAAGCAG ATGAAAATAA CCCCAGCAGT GAAAGTGCAC TTAATGAAGA ATGGCAGTCA 
GATAATAGTG ATGGAGATAT TGCCAGTGAA TGTGAAGAAT GTGATAGTGT CTTCAGCCAT 
TTGGAAGAGC TGAGATACAA CCTGGAACAG GAAATAGGCT TTGAGAAATT AATTGAAGTT 
TATAATAAAA TAAAGGCCAT ACATGAAGAT GAAGATGAAA ATATTGATAT TTGTTCAAGC 
ATAGTTCAGA CTATTCTGGG AAATGAACAC AAACACTTAT ATGCCAAAGT ACTTCACCTG 
GTCATGGCGG ATGGAGCCTA CCAGGAAGAT AATGACGAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015140860.1
CDS	1005..4706
Translation	MDKYIKVQKIGEGSFGKAFLVKDKENSQQYVIKEINISKMSNKEREESRREVAVLANMKHPNIVLYRESFEENGCLYIVMDYCEGGDLFKKINAQKGVLFSEDQILDWFVQICLALKHIHDRKILHRDIKSQNIFLTKDGTIQLGDFGIARVLNSTVELARTCIGTPYYLSPEICQNRPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIISGSFPPVSVHYSYDLRNLLSQLFKRNPRDRPSVNSILEKNFIAKRVEKFLTPQLIAEEFSHKVFHKFGPHAGPAKRPAQGHISASIAPVQKITKPAAKYGVPLAMKKCYDAPKKLHEKKSLIKLRQAFPTPKKKIIPGEERRKMFEEPSKKKRLELPEKEKRQRDQISLLKAEEMRRLEKERMERINRAREQGWRNVLGSGGSGDVKAPYYGGGGGVGPFPASFRGQYEHYHAIFDQMQKETFKVTKERQAKWRAEVQGQEAVERGIPPGVRPGVPKGPAGHHHLPDADAIRKKVKRLKEVSKQASANRQKGWLPAERAKQVEEFWQRKREAMQNKARAEGHMGTLQNVADIYGGRPMSARGRKSRNKEEEEYLARLRQIRLQNFNERQQIKAKLRGEKNEAPSSDGQESSEEAEMKRKKIEAQKAQANARAAVLKEQLERKRKEAYEREKRAWEEHLIAKGVKNPNVPFHVGAAGHTPVHGIQEPGVSPPKQQLPVKPISMTSALKEVGVDGNVTAAQEVKEEIKKPDLTMQNKRQILKRLNQNLKAQEDEKGRKDDKNISESDGSEDGKEEQEDNHPLLGDRKRWASRASDLVVPLAQLSMEDSLSGTDRQTMGEVIRLDIGEPRRKVWGKSPTDSVLKILGEAELQLPTELLEEIDVINEPDDLEAVMLKETEEDKKHQSKEFPIAVNEVWVKEKQDEAQQDARNEAAFEKEVVLDEVQSQNEAPEETCSSDSLQPEPLFQRVIQPPAVPTASPVLSSQSSQEEPFVPRSRSISPAKNKGKSSLFFGLSTGLFDANNPKMLRTCSLPDLYKLYRTLVDVPSIADVQHQHSLEIDDTEDEQAKEGPSDSEDIMFGDADTDLQELRASMEQLLREQPSEELSEEEESTLKANVIKCMTNGTELDEADENNPSSESALNEEWQSDNSDGDIASECEECDSVFSHLEELRYNLEQEIGFEKLIEVYNKIKAIHEDEDENIDICSSIVQTILGNEHKHLYAKVLHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_015285374.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus NIMA-related kinase 1 (NEK1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015285374.1

ORF Insert Sequence:

ATGGATAAAT ACATTAAAGT GCAAAAAATT GGAGAAGGAT CCTTTGGGAA AGCATTTCTT 
GTTAAAGATA AAGAAAATAG CCAGCAGTAT GTTATTAAAG AAATTAACAT ATCTAAGATG 
TCAAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TACTGGCCAA CATGAAACAT 
CCAAATATTG TCCTGTATAG GGAGTCGTTT GAAGAAAATG GTTGTCTCTA CATAGTGATG 
GATTACTGTG AAGGAGGAGA TCTGTTCAAA AAAATAAATG CCCAGAAAGG AGTCCTGTTC 
TCTGAGGATC AGATTCTTGA TTGGTTTGTA CAGATCTGTT TGGCACTGAA ACATATACAT 
GATAGAAAAA TCTTACATCG CGACATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA 
ACAATACAGC TGGGAGATTT TGGAATCGCG AGAGTTCTGA ACAGTACTGT GGAATTGGCT 
CGCACTTGCA TAGGAACACC ATACTATTTA TCACCTGAAA TATGCCAGAA CAGGCCTTAC 
AACAATAAAA GTGATATCTG GGCCCTTGGT TGCGTTCTCT ATGAAATGTG CACACTTAAG 
CATGCATTTG AAGCTGGTAA CATGAAGAAC TTGGTACTGA AGATAATCTC TGGATCTTTC 
CCTCCTGTTT CTGTGCATTA TTCCTATGAC CTACGTAATC TACTGTCACA GTTATTTAAA 
AGGAATCCTA GGGATAGACC ATCAGTGAAC TCCATATTGG AGAAAAATTT CATAGCCAAA 
CGGGTTGAAA AATTTCTCAC ACCGCAGCTT ATTGCAGAAG AATTCAGTCA CAAAGTGTTT 
CACAAGTTTG GACCACATGC TGGACCAGCT AAAAGACCAG CTCAAGGACA TATCTCGGCT 
TCAATTGCAC CAGTGCAGAA AATTACCAAA CCTGCTGCAA AATACGGAGT GCCTTTAGCG 
ATGAAGAAGT GTTATGACGC ACCCAAAAAG CTTCATGAGA AAAAGTCATT GATTAAACTT 
AGGCAGGCCT TTCCAACTCC AAAGAAGAAA ATTATTCCAG GAGAAGAAAG GAGGAAAATG 
TTTGAGGAAC CTTCAAAAAA GAAAAGGTTA GAATTGCCTG AAAAAGAAAA ACGCCAGAGA 
GATCAGATCA GTTTACTGAA GGCAGAGGAA ATGAGAAGAT TGGAAAAAGA AAGGATGGAA 
CGAATAAACA GAGCCAGGGA GCAAGGATGG AGGAATGTGC TTGGTTCAGG TGGAAGTGGT 
GATGTTAAGG CACCATATTA TGGCGGTGGA GGTGGTGTTG GTCCTTTTCC TGCCTCTTTC 
AGAGGACAGT ATGAGCACTA TCATGCTATT TTTGACCAGA TGCAAAAGGA AACCTTCAAA 
GTAACTAAAG AGAGGCAAGC CAAATGGAGG GCGGAGGTAC AAGGCCAAGA AGCTGTTGAG 
AGAGGAATTC CACCTGGAGT ACGTCCAGGA GTTCCTAAGG GGCCTGCAGG TCATCACCAT 
TTACCTGATG CTGATGCAAT TAGGAAAAAA GTGAAAAGGT TGAAGGAGGT GTCTAAACAA 
GCCAGTGCAA ACAGGCAGAA AGGATGGCTA CCTGCAGAAA GAGCAAAGCA AGTTGAAGAA 
TTCTGGCAAC GAAAGAGGGA AGCCATGCAA AACAAAGCTC GTGCTGAGGG ACACATGGGT 
ACACTGCAAA ACGTGGCAGA TATCTATGGA GGCAGGCCCA TGTCTGCAAG AGGAAGGAAA 
TCCAGAAACA AGGAAGAAGA GGAATATTTG GCAAGATTAA GACAGATACG ACTACAAAAC 
TTCAATGAGC GTCAACAGAT TAAAGCAAAA CTTCGTGGAG AGAAGAATGA AGCTCCCAGT 
TCTGATGGAC AAGAGTCAAG TGAGGAAGCA GAAATGAAAC GCAAAAAGAT AGAAGCACAG 
AAGGCCCAAG CAAATGCTCG AGCTGCAGTT CTGAAAGAAC AGTTAGAGCG AAAGAGGAAG 
GAGGCATATG AAAGAGAGAA AAGAGCTTGG GAAGAACATC TGATAGCAAA AGGAGTAAAG 
AATCCTAATG TGCCTTTTCA TGTGGGAGCA GCAGGACACA CTCCTGTGCA TGGAATACAA 
GAGCCTGGAG TGTCTCCTCC TAAGCAACAG CTTCCAGTTA AGCCCATCTC CATGACTTCT 
GCTTTGAAAG AAGTTGGCGT GGATGGAAAT GTAACCGCTG CCCAGGAGGT TAAAGAGGAA 
ATAAAAAAGC CAGATCTTAC AATGCAGAAC AAGAGGCAGA TACTGAAAAG ATTGAATCAA 
AATCTTAAAG CTCAGGAAGA TGAGAAAGGA AGAAAGGACG ATAAAAATAT CTCTGAGTCA 
GATGGGTCTG AAGATGGTAA AGAAGAGCAA GAAGATAACC ATCCACTTCT GGGAGATCGC 
AAAAGATGGG CATCAAGGGC ATCAGATTTG GTAGTTCCTT TAGCTCAGTT ATCAATGGAA 
GATTCTCTCT CTGGAACAGA CCGGCAAACT ATGGGGGAAG TAATCAGGCT AGATATTGGG 
GAACCCCGCA GGAAAGTCTG GGGCAAAAGC CCTACTGATT CGGTCCTGAA GATTCTAGGA 
GAAGCGGAGT TGCAGCTACC AACTGAATTA CTGGAAGAAA TAGATGTAAT AAATGAGCCT 
GATGATTTGG AAGCAGTGAT GTTGAAAGAA ACAGAAGAAG ATAAAAAGCA CCAGAGCAAG 
GAATTTCCCA TTGCTGTTAA TGAAGTGTGG GTGAAAGAAA AACAGGATGA GGCACAACAG 
GATGCAAGAA ATGAAGCAGC TTTTGAGAAA GAAGTAGTGC TTGATGAAGT GCAATCACAA 
AATGAAGCTC CAGAAGAAAC TTGTTCCAGT GACTCTCTGC AACCTGAACC GTTATTCCAG 
AGAGTAATAC AGCCCCCAGC TGTACCTACA GCCTCACCAG TTCTATCATC TCAGTCTTCC 
CAAGAAGAGC CTTTTGTACC TCGTTCACGT TCCATATCGC CAGCAAAAAA TAAAGGGAAA 
AGTTCATTGT TTTTTGGACT TTCTACAGGT CTGTTTGATG CAAACAACCC AAAGATGTTG 
AGAACGTGTT CACTCCCGGA TCTTTACAAA CTGTACAGAA CCTTAGTTGA TGTTCCCAGC 
ATAGCAGATG TACAGCATCA ACACAGTCTT GAAATAGATG ATACGGAAGA TGAGCAAGCC 
AAAGAAGGAC CCTCTGATTC TGAGGATATC ATGTTTGGGG ACGCCGATAC AGATTTGCAG 
GAACTCCGGG CCTCAATGGA ACAATTACTT AGGGAGCAGC CTAGTGAGGA ATTAAGTGAA 
GAGGAGGAAT CTACTTTAAA GGCTAACGTC ATCAAATGCA TGACAAATGG TACAGAGCTG 
GATGAAGCAG ATGAAAATAA CCCCAGCAGT GAAAGTGCAC TTAATGAAGA ATGGCAGTCA 
GATAATAGTG ATGGAGATAT TGCCAGTGAA TGTGAAGAAT GTGATAGTGT CTTCAGCCAT 
TTGGAAGAGC TGAGATACAA CCTGGAACAG GAAATAGGCT TTGAGAAATT AATTGAAGTT 
TATAATAAAA TAAAGGCCAT ACATGAAGAT GAAGATGAAA ATATTGATAT TTGTTCAAGC 
ATAGTTCAGA CTATTCTGGG AAATGAACAC AAACACTTAT ATGCCAAAGT ACTTCACCTG 
GTCATGGCGG ATGGAGCCTA CCAGGAAGAT AATGACGAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_015285376.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015140862.1 Gallus gallus NIMA related kinase 1 (NEK1), transcript variant X2, mRNA.

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CloneID	OGa07767
Clone ID Related Accession (Same CDS sequence)	XM_015285374.2 , XM_015285375.1 , XM_015285376.1 , XM_420401.5 , XM_420401.6 , XM_015285374.1 , XM_015285376.2 , XM_015285375.2
Accession Version	XM_015285376.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3702bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	serine/threonine-protein kinase Nek1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015285376.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAAAT ACATTAAAGT GCAAAAAATT GGAGAAGGAT CCTTTGGGAA AGCATTTCTT 
GTTAAAGATA AAGAAAATAG CCAGCAGTAT GTTATTAAAG AAATTAACAT ATCTAAGATG 
TCAAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TACTGGCCAA CATGAAACAT 
CCAAATATTG TCCTGTATAG GGAGTCGTTT GAAGAAAATG GTTGTCTCTA CATAGTGATG 
GATTACTGTG AAGGAGGAGA TCTGTTCAAA AAAATAAATG CCCAGAAAGG AGTCCTGTTC 
TCTGAGGATC AGATTCTTGA TTGGTTTGTA CAGATCTGTT TGGCACTGAA ACATATACAT 
GATAGAAAAA TCTTACATCG CGACATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA 
ACAATACAGC TGGGAGATTT TGGAATCGCG AGAGTTCTGA ACAGTACTGT GGAATTGGCT 
CGCACTTGCA TAGGAACACC ATACTATTTA TCACCTGAAA TATGCCAGAA CAGGCCTTAC 
AACAATAAAA GTGATATCTG GGCCCTTGGT TGCGTTCTCT ATGAAATGTG CACACTTAAG 
CATGCATTTG AAGCTGGTAA CATGAAGAAC TTGGTACTGA AGATAATCTC TGGATCTTTC 
CCTCCTGTTT CTGTGCATTA TTCCTATGAC CTACGTAATC TACTGTCACA GTTATTTAAA 
AGGAATCCTA GGGATAGACC ATCAGTGAAC TCCATATTGG AGAAAAATTT CATAGCCAAA 
CGGGTTGAAA AATTTCTCAC ACCGCAGCTT ATTGCAGAAG AATTCAGTCA CAAAGTGTTT 
CACAAGTTTG GACCACATGC TGGACCAGCT AAAAGACCAG CTCAAGGACA TATCTCGGCT 
TCAATTGCAC CAGTGCAGAA AATTACCAAA CCTGCTGCAA AATACGGAGT GCCTTTAGCG 
ATGAAGAAGT GTTATGACGC ACCCAAAAAG CTTCATGAGA AAAAGTCATT GATTAAACTT 
AGGCAGGCCT TTCCAACTCC AAAGAAGAAA ATTATTCCAG GAGAAGAAAG GAGGAAAATG 
TTTGAGGAAC CTTCAAAAAA GAAAAGGTTA GAATTGCCTG AAAAAGAAAA ACGCCAGAGA 
GATCAGATCA GTTTACTGAA GGCAGAGGAA ATGAGAAGAT TGGAAAAAGA AAGGATGGAA 
CGAATAAACA GAGCCAGGGA GCAAGGATGG AGGAATGTGC TTGGTTCAGG TGGAAGTGGT 
GATGTTAAGG CACCATATTA TGGCGGTGGA GGTGGTGTTG GTCCTTTTCC TGCCTCTTTC 
AGAGGACAGT ATGAGCACTA TCATGCTATT TTTGACCAGA TGCAAAAGGA AACCTTCAAA 
GTAACTAAAG AGAGGCAAGC CAAATGGAGG GCGGAGGTAC AAGGCCAAGA AGCTGTTGAG 
AGAGGAATTC CACCTGGAGT ACGTCCAGGA GTTCCTAAGG GGCCTGCAGG TCATCACCAT 
TTACCTGATG CTGATGCAAT TAGGAAAAAA GTGAAAAGGT TGAAGGAGGT GTCTAAACAA 
GCCAGTGCAA ACAGGCAGAA AGGATGGCTA CCTGCAGAAA GAGCAAAGCA AGTTGAAGAA 
TTCTGGCAAC GAAAGAGGGA AGCCATGCAA AACAAAGCTC GTGCTGAGGG ACACATGGGT 
ACACTGCAAA ACGTGGCAGA TATCTATGGA GGCAGGCCCA TGTCTGCAAG AGGAAGGAAA 
TCCAGAAACA AGGAAGAAGA GGAATATTTG GCAAGATTAA GACAGATACG ACTACAAAAC 
TTCAATGAGC GTCAACAGAT TAAAGCAAAA CTTCGTGGAG AGAAGAATGA AGCTCCCAGT 
TCTGATGGAC AAGAGTCAAG TGAGGAAGCA GAAATGAAAC GCAAAAAGAT AGAAGCACAG 
AAGGCCCAAG CAAATGCTCG AGCTGCAGTT CTGAAAGAAC AGTTAGAGCG AAAGAGGAAG 
GAGGCATATG AAAGAGAGAA AAGAGCTTGG GAAGAACATC TGATAGCAAA AGGAGTAAAG 
AATCCTAATG TGCCTTTTCA TGTGGGAGCA GCAGGACACA CTCCTGTGCA TGGAATACAA 
GAGCCTGGAG TGTCTCCTCC TAAGCAACAG CTTCCAGTTA AGCCCATCTC CATGACTTCT 
GCTTTGAAAG AAGTTGGCGT GGATGGAAAT GTAACCGCTG CCCAGGAGGT TAAAGAGGAA 
ATAAAAAAGC CAGATCTTAC AATGCAGAAC AAGAGGCAGA TACTGAAAAG ATTGAATCAA 
AATCTTAAAG CTCAGGAAGA TGAGAAAGGA AGAAAGGACG ATAAAAATAT CTCTGAGTCA 
GATGGGTCTG AAGATGGTAA AGAAGAGCAA GAAGATAACC ATCCACTTCT GGGAGATCGC 
AAAAGATGGG CATCAAGGGC ATCAGATTTG GTAGTTCCTT TAGCTCAGTT ATCAATGGAA 
GATTCTCTCT CTGGAACAGA CCGGCAAACT ATGGGGGAAG TAATCAGGCT AGATATTGGG 
GAACCCCGCA GGAAAGTCTG GGGCAAAAGC CCTACTGATT CGGTCCTGAA GATTCTAGGA 
GAAGCGGAGT TGCAGCTACC AACTGAATTA CTGGAAGAAA TAGATGTAAT AAATGAGCCT 
GATGATTTGG AAGCAGTGAT GTTGAAAGAA ACAGAAGAAG ATAAAAAGCA CCAGAGCAAG 
GAATTTCCCA TTGCTGTTAA TGAAGTGTGG GTGAAAGAAA AACAGGATGA GGCACAACAG 
GATGCAAGAA ATGAAGCAGC TTTTGAGAAA GAAGTAGTGC TTGATGAAGT GCAATCACAA 
AATGAAGCTC CAGAAGAAAC TTGTTCCAGT GACTCTCTGC AACCTGAACC GTTATTCCAG 
AGAGTAATAC AGCCCCCAGC TGTACCTACA GCCTCACCAG TTCTATCATC TCAGTCTTCC 
CAAGAAGAGC CTTTTGTACC TCGTTCACGT TCCATATCGC CAGCAAAAAA TAAAGGGAAA 
AGTTCATTGT TTTTTGGACT TTCTACAGGT CTGTTTGATG CAAACAACCC AAAGATGTTG 
AGAACGTGTT CACTCCCGGA TCTTTACAAA CTGTACAGAA CCTTAGTTGA TGTTCCCAGC 
ATAGCAGATG TACAGCATCA ACACAGTCTT GAAATAGATG ATACGGAAGA TGAGCAAGCC 
AAAGAAGGAC CCTCTGATTC TGAGGATATC ATGTTTGGGG ACGCCGATAC AGATTTGCAG 
GAACTCCGGG CCTCAATGGA ACAATTACTT AGGGAGCAGC CTAGTGAGGA ATTAAGTGAA 
GAGGAGGAAT CTACTTTAAA GGCTAACGTC ATCAAATGCA TGACAAATGG TACAGAGCTG 
GATGAAGCAG ATGAAAATAA CCCCAGCAGT GAAAGTGCAC TTAATGAAGA ATGGCAGTCA 
GATAATAGTG ATGGAGATAT TGCCAGTGAA TGTGAAGAAT GTGATAGTGT CTTCAGCCAT 
TTGGAAGAGC TGAGATACAA CCTGGAACAG GAAATAGGCT TTGAGAAATT AATTGAAGTT 
TATAATAAAA TAAAGGCCAT ACATGAAGAT GAAGATGAAA ATATTGATAT TTGTTCAAGC 
ATAGTTCAGA CTATTCTGGG AAATGAACAC AAACACTTAT ATGCCAAAGT ACTTCACCTG 
GTCATGGCGG ATGGAGCCTA CCAGGAAGAT AATGACGAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015140862.1
CDS	832..4533
Translation	MDKYIKVQKIGEGSFGKAFLVKDKENSQQYVIKEINISKMSNKEREESRREVAVLANMKHPNIVLYRESFEENGCLYIVMDYCEGGDLFKKINAQKGVLFSEDQILDWFVQICLALKHIHDRKILHRDIKSQNIFLTKDGTIQLGDFGIARVLNSTVELARTCIGTPYYLSPEICQNRPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIISGSFPPVSVHYSYDLRNLLSQLFKRNPRDRPSVNSILEKNFIAKRVEKFLTPQLIAEEFSHKVFHKFGPHAGPAKRPAQGHISASIAPVQKITKPAAKYGVPLAMKKCYDAPKKLHEKKSLIKLRQAFPTPKKKIIPGEERRKMFEEPSKKKRLELPEKEKRQRDQISLLKAEEMRRLEKERMERINRAREQGWRNVLGSGGSGDVKAPYYGGGGGVGPFPASFRGQYEHYHAIFDQMQKETFKVTKERQAKWRAEVQGQEAVERGIPPGVRPGVPKGPAGHHHLPDADAIRKKVKRLKEVSKQASANRQKGWLPAERAKQVEEFWQRKREAMQNKARAEGHMGTLQNVADIYGGRPMSARGRKSRNKEEEEYLARLRQIRLQNFNERQQIKAKLRGEKNEAPSSDGQESSEEAEMKRKKIEAQKAQANARAAVLKEQLERKRKEAYEREKRAWEEHLIAKGVKNPNVPFHVGAAGHTPVHGIQEPGVSPPKQQLPVKPISMTSALKEVGVDGNVTAAQEVKEEIKKPDLTMQNKRQILKRLNQNLKAQEDEKGRKDDKNISESDGSEDGKEEQEDNHPLLGDRKRWASRASDLVVPLAQLSMEDSLSGTDRQTMGEVIRLDIGEPRRKVWGKSPTDSVLKILGEAELQLPTELLEEIDVINEPDDLEAVMLKETEEDKKHQSKEFPIAVNEVWVKEKQDEAQQDARNEAAFEKEVVLDEVQSQNEAPEETCSSDSLQPEPLFQRVIQPPAVPTASPVLSSQSSQEEPFVPRSRSISPAKNKGKSSLFFGLSTGLFDANNPKMLRTCSLPDLYKLYRTLVDVPSIADVQHQHSLEIDDTEDEQAKEGPSDSEDIMFGDADTDLQELRASMEQLLREQPSEELSEEEESTLKANVIKCMTNGTELDEADENNPSSESALNEEWQSDNSDGDIASECEECDSVFSHLEELRYNLEQEIGFEKLIEVYNKIKAIHEDEDENIDICSSIVQTILGNEHKHLYAKVLHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_015285376.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus NIMA related kinase 1 (NEK1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015285376.2

ORF Insert Sequence:

ATGGATAAAT ACATTAAAGT GCAAAAAATT GGAGAAGGAT CCTTTGGGAA AGCATTTCTT 
GTTAAAGATA AAGAAAATAG CCAGCAGTAT GTTATTAAAG AAATTAACAT ATCTAAGATG 
TCAAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TACTGGCCAA CATGAAACAT 
CCAAATATTG TCCTGTATAG GGAGTCGTTT GAAGAAAATG GTTGTCTCTA CATAGTGATG 
GATTACTGTG AAGGAGGAGA TCTGTTCAAA AAAATAAATG CCCAGAAAGG AGTCCTGTTC 
TCTGAGGATC AGATTCTTGA TTGGTTTGTA CAGATCTGTT TGGCACTGAA ACATATACAT 
GATAGAAAAA TCTTACATCG CGACATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA 
ACAATACAGC TGGGAGATTT TGGAATCGCG AGAGTTCTGA ACAGTACTGT GGAATTGGCT 
CGCACTTGCA TAGGAACACC ATACTATTTA TCACCTGAAA TATGCCAGAA CAGGCCTTAC 
AACAATAAAA GTGATATCTG GGCCCTTGGT TGCGTTCTCT ATGAAATGTG CACACTTAAG 
CATGCATTTG AAGCTGGTAA CATGAAGAAC TTGGTACTGA AGATAATCTC TGGATCTTTC 
CCTCCTGTTT CTGTGCATTA TTCCTATGAC CTACGTAATC TACTGTCACA GTTATTTAAA 
AGGAATCCTA GGGATAGACC ATCAGTGAAC TCCATATTGG AGAAAAATTT CATAGCCAAA 
CGGGTTGAAA AATTTCTCAC ACCGCAGCTT ATTGCAGAAG AATTCAGTCA CAAAGTGTTT 
CACAAGTTTG GACCACATGC TGGACCAGCT AAAAGACCAG CTCAAGGACA TATCTCGGCT 
TCAATTGCAC CAGTGCAGAA AATTACCAAA CCTGCTGCAA AATACGGAGT GCCTTTAGCG 
ATGAAGAAGT GTTATGACGC ACCCAAAAAG CTTCATGAGA AAAAGTCATT GATTAAACTT 
AGGCAGGCCT TTCCAACTCC AAAGAAGAAA ATTATTCCAG GAGAAGAAAG GAGGAAAATG 
TTTGAGGAAC CTTCAAAAAA GAAAAGGTTA GAATTGCCTG AAAAAGAAAA ACGCCAGAGA 
GATCAGATCA GTTTACTGAA GGCAGAGGAA ATGAGAAGAT TGGAAAAAGA AAGGATGGAA 
CGAATAAACA GAGCCAGGGA GCAAGGATGG AGGAATGTGC TTGGTTCAGG TGGAAGTGGT 
GATGTTAAGG CACCATATTA TGGCGGTGGA GGTGGTGTTG GTCCTTTTCC TGCCTCTTTC 
AGAGGACAGT ATGAGCACTA TCATGCTATT TTTGACCAGA TGCAAAAGGA AACCTTCAAA 
GTAACTAAAG AGAGGCAAGC CAAATGGAGG GCGGAGGTAC AAGGCCAAGA AGCTGTTGAG 
AGAGGAATTC CACCTGGAGT ACGTCCAGGA GTTCCTAAGG GGCCTGCAGG TCATCACCAT 
TTACCTGATG CTGATGCAAT TAGGAAAAAA GTGAAAAGGT TGAAGGAGGT GTCTAAACAA 
GCCAGTGCAA ACAGGCAGAA AGGATGGCTA CCTGCAGAAA GAGCAAAGCA AGTTGAAGAA 
TTCTGGCAAC GAAAGAGGGA AGCCATGCAA AACAAAGCTC GTGCTGAGGG ACACATGGGT 
ACACTGCAAA ACGTGGCAGA TATCTATGGA GGCAGGCCCA TGTCTGCAAG AGGAAGGAAA 
TCCAGAAACA AGGAAGAAGA GGAATATTTG GCAAGATTAA GACAGATACG ACTACAAAAC 
TTCAATGAGC GTCAACAGAT TAAAGCAAAA CTTCGTGGAG AGAAGAATGA AGCTCCCAGT 
TCTGATGGAC AAGAGTCAAG TGAGGAAGCA GAAATGAAAC GCAAAAAGAT AGAAGCACAG 
AAGGCCCAAG CAAATGCTCG AGCTGCAGTT CTGAAAGAAC AGTTAGAGCG AAAGAGGAAG 
GAGGCATATG AAAGAGAGAA AAGAGCTTGG GAAGAACATC TGATAGCAAA AGGAGTAAAG 
AATCCTAATG TGCCTTTTCA TGTGGGAGCA GCAGGACACA CTCCTGTGCA TGGAATACAA 
GAGCCTGGAG TGTCTCCTCC TAAGCAACAG CTTCCAGTTA AGCCCATCTC CATGACTTCT 
GCTTTGAAAG AAGTTGGCGT GGATGGAAAT GTAACCGCTG CCCAGGAGGT TAAAGAGGAA 
ATAAAAAAGC CAGATCTTAC AATGCAGAAC AAGAGGCAGA TACTGAAAAG ATTGAATCAA 
AATCTTAAAG CTCAGGAAGA TGAGAAAGGA AGAAAGGACG ATAAAAATAT CTCTGAGTCA 
GATGGGTCTG AAGATGGTAA AGAAGAGCAA GAAGATAACC ATCCACTTCT GGGAGATCGC 
AAAAGATGGG CATCAAGGGC ATCAGATTTG GTAGTTCCTT TAGCTCAGTT ATCAATGGAA 
GATTCTCTCT CTGGAACAGA CCGGCAAACT ATGGGGGAAG TAATCAGGCT AGATATTGGG 
GAACCCCGCA GGAAAGTCTG GGGCAAAAGC CCTACTGATT CGGTCCTGAA GATTCTAGGA 
GAAGCGGAGT TGCAGCTACC AACTGAATTA CTGGAAGAAA TAGATGTAAT AAATGAGCCT 
GATGATTTGG AAGCAGTGAT GTTGAAAGAA ACAGAAGAAG ATAAAAAGCA CCAGAGCAAG 
GAATTTCCCA TTGCTGTTAA TGAAGTGTGG GTGAAAGAAA AACAGGATGA GGCACAACAG 
GATGCAAGAA ATGAAGCAGC TTTTGAGAAA GAAGTAGTGC TTGATGAAGT GCAATCACAA 
AATGAAGCTC CAGAAGAAAC TTGTTCCAGT GACTCTCTGC AACCTGAACC GTTATTCCAG 
AGAGTAATAC AGCCCCCAGC TGTACCTACA GCCTCACCAG TTCTATCATC TCAGTCTTCC 
CAAGAAGAGC CTTTTGTACC TCGTTCACGT TCCATATCGC CAGCAAAAAA TAAAGGGAAA 
AGTTCATTGT TTTTTGGACT TTCTACAGGT CTGTTTGATG CAAACAACCC AAAGATGTTG 
AGAACGTGTT CACTCCCGGA TCTTTACAAA CTGTACAGAA CCTTAGTTGA TGTTCCCAGC 
ATAGCAGATG TACAGCATCA ACACAGTCTT GAAATAGATG ATACGGAAGA TGAGCAAGCC 
AAAGAAGGAC CCTCTGATTC TGAGGATATC ATGTTTGGGG ACGCCGATAC AGATTTGCAG 
GAACTCCGGG CCTCAATGGA ACAATTACTT AGGGAGCAGC CTAGTGAGGA ATTAAGTGAA 
GAGGAGGAAT CTACTTTAAA GGCTAACGTC ATCAAATGCA TGACAAATGG TACAGAGCTG 
GATGAAGCAG ATGAAAATAA CCCCAGCAGT GAAAGTGCAC TTAATGAAGA ATGGCAGTCA 
GATAATAGTG ATGGAGATAT TGCCAGTGAA TGTGAAGAAT GTGATAGTGT CTTCAGCCAT 
TTGGAAGAGC TGAGATACAA CCTGGAACAG GAAATAGGCT TTGAGAAATT AATTGAAGTT 
TATAATAAAA TAAAGGCCAT ACATGAAGAT GAAGATGAAA ATATTGATAT TTGTTCAAGC 
ATAGTTCAGA CTATTCTGGG AAATGAACAC AAACACTTAT ATGCCAAAGT ACTTCACCTG 
GTCATGGCGG ATGGAGCCTA CCAGGAAGAT AATGACGAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_015285375.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015140861.1 Gallus gallus NIMA related kinase 1 (NEK1), transcript variant X3, mRNA.

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CloneID	OGa07767
Clone ID Related Accession (Same CDS sequence)	XM_015285374.2 , XM_015285375.1 , XM_015285376.1 , XM_420401.5 , XM_420401.6 , XM_015285374.1 , XM_015285376.2 , XM_015285375.2
Accession Version	XM_015285375.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3702bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	serine/threonine-protein kinase Nek1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015285375.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAAAT ACATTAAAGT GCAAAAAATT GGAGAAGGAT CCTTTGGGAA AGCATTTCTT 
GTTAAAGATA AAGAAAATAG CCAGCAGTAT GTTATTAAAG AAATTAACAT ATCTAAGATG 
TCAAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TACTGGCCAA CATGAAACAT 
CCAAATATTG TCCTGTATAG GGAGTCGTTT GAAGAAAATG GTTGTCTCTA CATAGTGATG 
GATTACTGTG AAGGAGGAGA TCTGTTCAAA AAAATAAATG CCCAGAAAGG AGTCCTGTTC 
TCTGAGGATC AGATTCTTGA TTGGTTTGTA CAGATCTGTT TGGCACTGAA ACATATACAT 
GATAGAAAAA TCTTACATCG CGACATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA 
ACAATACAGC TGGGAGATTT TGGAATCGCG AGAGTTCTGA ACAGTACTGT GGAATTGGCT 
CGCACTTGCA TAGGAACACC ATACTATTTA TCACCTGAAA TATGCCAGAA CAGGCCTTAC 
AACAATAAAA GTGATATCTG GGCCCTTGGT TGCGTTCTCT ATGAAATGTG CACACTTAAG 
CATGCATTTG AAGCTGGTAA CATGAAGAAC TTGGTACTGA AGATAATCTC TGGATCTTTC 
CCTCCTGTTT CTGTGCATTA TTCCTATGAC CTACGTAATC TACTGTCACA GTTATTTAAA 
AGGAATCCTA GGGATAGACC ATCAGTGAAC TCCATATTGG AGAAAAATTT CATAGCCAAA 
CGGGTTGAAA AATTTCTCAC ACCGCAGCTT ATTGCAGAAG AATTCAGTCA CAAAGTGTTT 
CACAAGTTTG GACCACATGC TGGACCAGCT AAAAGACCAG CTCAAGGACA TATCTCGGCT 
TCAATTGCAC CAGTGCAGAA AATTACCAAA CCTGCTGCAA AATACGGAGT GCCTTTAGCG 
ATGAAGAAGT GTTATGACGC ACCCAAAAAG CTTCATGAGA AAAAGTCATT GATTAAACTT 
AGGCAGGCCT TTCCAACTCC AAAGAAGAAA ATTATTCCAG GAGAAGAAAG GAGGAAAATG 
TTTGAGGAAC CTTCAAAAAA GAAAAGGTTA GAATTGCCTG AAAAAGAAAA ACGCCAGAGA 
GATCAGATCA GTTTACTGAA GGCAGAGGAA ATGAGAAGAT TGGAAAAAGA AAGGATGGAA 
CGAATAAACA GAGCCAGGGA GCAAGGATGG AGGAATGTGC TTGGTTCAGG TGGAAGTGGT 
GATGTTAAGG CACCATATTA TGGCGGTGGA GGTGGTGTTG GTCCTTTTCC TGCCTCTTTC 
AGAGGACAGT ATGAGCACTA TCATGCTATT TTTGACCAGA TGCAAAAGGA AACCTTCAAA 
GTAACTAAAG AGAGGCAAGC CAAATGGAGG GCGGAGGTAC AAGGCCAAGA AGCTGTTGAG 
AGAGGAATTC CACCTGGAGT ACGTCCAGGA GTTCCTAAGG GGCCTGCAGG TCATCACCAT 
TTACCTGATG CTGATGCAAT TAGGAAAAAA GTGAAAAGGT TGAAGGAGGT GTCTAAACAA 
GCCAGTGCAA ACAGGCAGAA AGGATGGCTA CCTGCAGAAA GAGCAAAGCA AGTTGAAGAA 
TTCTGGCAAC GAAAGAGGGA AGCCATGCAA AACAAAGCTC GTGCTGAGGG ACACATGGGT 
ACACTGCAAA ACGTGGCAGA TATCTATGGA GGCAGGCCCA TGTCTGCAAG AGGAAGGAAA 
TCCAGAAACA AGGAAGAAGA GGAATATTTG GCAAGATTAA GACAGATACG ACTACAAAAC 
TTCAATGAGC GTCAACAGAT TAAAGCAAAA CTTCGTGGAG AGAAGAATGA AGCTCCCAGT 
TCTGATGGAC AAGAGTCAAG TGAGGAAGCA GAAATGAAAC GCAAAAAGAT AGAAGCACAG 
AAGGCCCAAG CAAATGCTCG AGCTGCAGTT CTGAAAGAAC AGTTAGAGCG AAAGAGGAAG 
GAGGCATATG AAAGAGAGAA AAGAGCTTGG GAAGAACATC TGATAGCAAA AGGAGTAAAG 
AATCCTAATG TGCCTTTTCA TGTGGGAGCA GCAGGACACA CTCCTGTGCA TGGAATACAA 
GAGCCTGGAG TGTCTCCTCC TAAGCAACAG CTTCCAGTTA AGCCCATCTC CATGACTTCT 
GCTTTGAAAG AAGTTGGCGT GGATGGAAAT GTAACCGCTG CCCAGGAGGT TAAAGAGGAA 
ATAAAAAAGC CAGATCTTAC AATGCAGAAC AAGAGGCAGA TACTGAAAAG ATTGAATCAA 
AATCTTAAAG CTCAGGAAGA TGAGAAAGGA AGAAAGGACG ATAAAAATAT CTCTGAGTCA 
GATGGGTCTG AAGATGGTAA AGAAGAGCAA GAAGATAACC ATCCACTTCT GGGAGATCGC 
AAAAGATGGG CATCAAGGGC ATCAGATTTG GTAGTTCCTT TAGCTCAGTT ATCAATGGAA 
GATTCTCTCT CTGGAACAGA CCGGCAAACT ATGGGGGAAG TAATCAGGCT AGATATTGGG 
GAACCCCGCA GGAAAGTCTG GGGCAAAAGC CCTACTGATT CGGTCCTGAA GATTCTAGGA 
GAAGCGGAGT TGCAGCTACC AACTGAATTA CTGGAAGAAA TAGATGTAAT AAATGAGCCT 
GATGATTTGG AAGCAGTGAT GTTGAAAGAA ACAGAAGAAG ATAAAAAGCA CCAGAGCAAG 
GAATTTCCCA TTGCTGTTAA TGAAGTGTGG GTGAAAGAAA AACAGGATGA GGCACAACAG 
GATGCAAGAA ATGAAGCAGC TTTTGAGAAA GAAGTAGTGC TTGATGAAGT GCAATCACAA 
AATGAAGCTC CAGAAGAAAC TTGTTCCAGT GACTCTCTGC AACCTGAACC GTTATTCCAG 
AGAGTAATAC AGCCCCCAGC TGTACCTACA GCCTCACCAG TTCTATCATC TCAGTCTTCC 
CAAGAAGAGC CTTTTGTACC TCGTTCACGT TCCATATCGC CAGCAAAAAA TAAAGGGAAA 
AGTTCATTGT TTTTTGGACT TTCTACAGGT CTGTTTGATG CAAACAACCC AAAGATGTTG 
AGAACGTGTT CACTCCCGGA TCTTTACAAA CTGTACAGAA CCTTAGTTGA TGTTCCCAGC 
ATAGCAGATG TACAGCATCA ACACAGTCTT GAAATAGATG ATACGGAAGA TGAGCAAGCC 
AAAGAAGGAC CCTCTGATTC TGAGGATATC ATGTTTGGGG ACGCCGATAC AGATTTGCAG 
GAACTCCGGG CCTCAATGGA ACAATTACTT AGGGAGCAGC CTAGTGAGGA ATTAAGTGAA 
GAGGAGGAAT CTACTTTAAA GGCTAACGTC ATCAAATGCA TGACAAATGG TACAGAGCTG 
GATGAAGCAG ATGAAAATAA CCCCAGCAGT GAAAGTGCAC TTAATGAAGA ATGGCAGTCA 
GATAATAGTG ATGGAGATAT TGCCAGTGAA TGTGAAGAAT GTGATAGTGT CTTCAGCCAT 
TTGGAAGAGC TGAGATACAA CCTGGAACAG GAAATAGGCT TTGAGAAATT AATTGAAGTT 
TATAATAAAA TAAAGGCCAT ACATGAAGAT GAAGATGAAA ATATTGATAT TTGTTCAAGC 
ATAGTTCAGA CTATTCTGGG AAATGAACAC AAACACTTAT ATGCCAAAGT ACTTCACCTG 
GTCATGGCGG ATGGAGCCTA CCAGGAAGAT AATGACGAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015140861.1
CDS	216..3917
Translation	MDKYIKVQKIGEGSFGKAFLVKDKENSQQYVIKEINISKMSNKEREESRREVAVLANMKHPNIVLYRESFEENGCLYIVMDYCEGGDLFKKINAQKGVLFSEDQILDWFVQICLALKHIHDRKILHRDIKSQNIFLTKDGTIQLGDFGIARVLNSTVELARTCIGTPYYLSPEICQNRPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIISGSFPPVSVHYSYDLRNLLSQLFKRNPRDRPSVNSILEKNFIAKRVEKFLTPQLIAEEFSHKVFHKFGPHAGPAKRPAQGHISASIAPVQKITKPAAKYGVPLAMKKCYDAPKKLHEKKSLIKLRQAFPTPKKKIIPGEERRKMFEEPSKKKRLELPEKEKRQRDQISLLKAEEMRRLEKERMERINRAREQGWRNVLGSGGSGDVKAPYYGGGGGVGPFPASFRGQYEHYHAIFDQMQKETFKVTKERQAKWRAEVQGQEAVERGIPPGVRPGVPKGPAGHHHLPDADAIRKKVKRLKEVSKQASANRQKGWLPAERAKQVEEFWQRKREAMQNKARAEGHMGTLQNVADIYGGRPMSARGRKSRNKEEEEYLARLRQIRLQNFNERQQIKAKLRGEKNEAPSSDGQESSEEAEMKRKKIEAQKAQANARAAVLKEQLERKRKEAYEREKRAWEEHLIAKGVKNPNVPFHVGAAGHTPVHGIQEPGVSPPKQQLPVKPISMTSALKEVGVDGNVTAAQEVKEEIKKPDLTMQNKRQILKRLNQNLKAQEDEKGRKDDKNISESDGSEDGKEEQEDNHPLLGDRKRWASRASDLVVPLAQLSMEDSLSGTDRQTMGEVIRLDIGEPRRKVWGKSPTDSVLKILGEAELQLPTELLEEIDVINEPDDLEAVMLKETEEDKKHQSKEFPIAVNEVWVKEKQDEAQQDARNEAAFEKEVVLDEVQSQNEAPEETCSSDSLQPEPLFQRVIQPPAVPTASPVLSSQSSQEEPFVPRSRSISPAKNKGKSSLFFGLSTGLFDANNPKMLRTCSLPDLYKLYRTLVDVPSIADVQHQHSLEIDDTEDEQAKEGPSDSEDIMFGDADTDLQELRASMEQLLREQPSEELSEEEESTLKANVIKCMTNGTELDEADENNPSSESALNEEWQSDNSDGDIASECEECDSVFSHLEELRYNLEQEIGFEKLIEVYNKIKAIHEDEDENIDICSSIVQTILGNEHKHLYAKVLHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_015285375.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus NIMA related kinase 1 (NEK1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015285375.2

ORF Insert Sequence:

ATGGATAAAT ACATTAAAGT GCAAAAAATT GGAGAAGGAT CCTTTGGGAA AGCATTTCTT 
GTTAAAGATA AAGAAAATAG CCAGCAGTAT GTTATTAAAG AAATTAACAT ATCTAAGATG 
TCAAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TACTGGCCAA CATGAAACAT 
CCAAATATTG TCCTGTATAG GGAGTCGTTT GAAGAAAATG GTTGTCTCTA CATAGTGATG 
GATTACTGTG AAGGAGGAGA TCTGTTCAAA AAAATAAATG CCCAGAAAGG AGTCCTGTTC 
TCTGAGGATC AGATTCTTGA TTGGTTTGTA CAGATCTGTT TGGCACTGAA ACATATACAT 
GATAGAAAAA TCTTACATCG CGACATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA 
ACAATACAGC TGGGAGATTT TGGAATCGCG AGAGTTCTGA ACAGTACTGT GGAATTGGCT 
CGCACTTGCA TAGGAACACC ATACTATTTA TCACCTGAAA TATGCCAGAA CAGGCCTTAC 
AACAATAAAA GTGATATCTG GGCCCTTGGT TGCGTTCTCT ATGAAATGTG CACACTTAAG 
CATGCATTTG AAGCTGGTAA CATGAAGAAC TTGGTACTGA AGATAATCTC TGGATCTTTC 
CCTCCTGTTT CTGTGCATTA TTCCTATGAC CTACGTAATC TACTGTCACA GTTATTTAAA 
AGGAATCCTA GGGATAGACC ATCAGTGAAC TCCATATTGG AGAAAAATTT CATAGCCAAA 
CGGGTTGAAA AATTTCTCAC ACCGCAGCTT ATTGCAGAAG AATTCAGTCA CAAAGTGTTT 
CACAAGTTTG GACCACATGC TGGACCAGCT AAAAGACCAG CTCAAGGACA TATCTCGGCT 
TCAATTGCAC CAGTGCAGAA AATTACCAAA CCTGCTGCAA AATACGGAGT GCCTTTAGCG 
ATGAAGAAGT GTTATGACGC ACCCAAAAAG CTTCATGAGA AAAAGTCATT GATTAAACTT 
AGGCAGGCCT TTCCAACTCC AAAGAAGAAA ATTATTCCAG GAGAAGAAAG GAGGAAAATG 
TTTGAGGAAC CTTCAAAAAA GAAAAGGTTA GAATTGCCTG AAAAAGAAAA ACGCCAGAGA 
GATCAGATCA GTTTACTGAA GGCAGAGGAA ATGAGAAGAT TGGAAAAAGA AAGGATGGAA 
CGAATAAACA GAGCCAGGGA GCAAGGATGG AGGAATGTGC TTGGTTCAGG TGGAAGTGGT 
GATGTTAAGG CACCATATTA TGGCGGTGGA GGTGGTGTTG GTCCTTTTCC TGCCTCTTTC 
AGAGGACAGT ATGAGCACTA TCATGCTATT TTTGACCAGA TGCAAAAGGA AACCTTCAAA 
GTAACTAAAG AGAGGCAAGC CAAATGGAGG GCGGAGGTAC AAGGCCAAGA AGCTGTTGAG 
AGAGGAATTC CACCTGGAGT ACGTCCAGGA GTTCCTAAGG GGCCTGCAGG TCATCACCAT 
TTACCTGATG CTGATGCAAT TAGGAAAAAA GTGAAAAGGT TGAAGGAGGT GTCTAAACAA 
GCCAGTGCAA ACAGGCAGAA AGGATGGCTA CCTGCAGAAA GAGCAAAGCA AGTTGAAGAA 
TTCTGGCAAC GAAAGAGGGA AGCCATGCAA AACAAAGCTC GTGCTGAGGG ACACATGGGT 
ACACTGCAAA ACGTGGCAGA TATCTATGGA GGCAGGCCCA TGTCTGCAAG AGGAAGGAAA 
TCCAGAAACA AGGAAGAAGA GGAATATTTG GCAAGATTAA GACAGATACG ACTACAAAAC 
TTCAATGAGC GTCAACAGAT TAAAGCAAAA CTTCGTGGAG AGAAGAATGA AGCTCCCAGT 
TCTGATGGAC AAGAGTCAAG TGAGGAAGCA GAAATGAAAC GCAAAAAGAT AGAAGCACAG 
AAGGCCCAAG CAAATGCTCG AGCTGCAGTT CTGAAAGAAC AGTTAGAGCG AAAGAGGAAG 
GAGGCATATG AAAGAGAGAA AAGAGCTTGG GAAGAACATC TGATAGCAAA AGGAGTAAAG 
AATCCTAATG TGCCTTTTCA TGTGGGAGCA GCAGGACACA CTCCTGTGCA TGGAATACAA 
GAGCCTGGAG TGTCTCCTCC TAAGCAACAG CTTCCAGTTA AGCCCATCTC CATGACTTCT 
GCTTTGAAAG AAGTTGGCGT GGATGGAAAT GTAACCGCTG CCCAGGAGGT TAAAGAGGAA 
ATAAAAAAGC CAGATCTTAC AATGCAGAAC AAGAGGCAGA TACTGAAAAG ATTGAATCAA 
AATCTTAAAG CTCAGGAAGA TGAGAAAGGA AGAAAGGACG ATAAAAATAT CTCTGAGTCA 
GATGGGTCTG AAGATGGTAA AGAAGAGCAA GAAGATAACC ATCCACTTCT GGGAGATCGC 
AAAAGATGGG CATCAAGGGC ATCAGATTTG GTAGTTCCTT TAGCTCAGTT ATCAATGGAA 
GATTCTCTCT CTGGAACAGA CCGGCAAACT ATGGGGGAAG TAATCAGGCT AGATATTGGG 
GAACCCCGCA GGAAAGTCTG GGGCAAAAGC CCTACTGATT CGGTCCTGAA GATTCTAGGA 
GAAGCGGAGT TGCAGCTACC AACTGAATTA CTGGAAGAAA TAGATGTAAT AAATGAGCCT 
GATGATTTGG AAGCAGTGAT GTTGAAAGAA ACAGAAGAAG ATAAAAAGCA CCAGAGCAAG 
GAATTTCCCA TTGCTGTTAA TGAAGTGTGG GTGAAAGAAA AACAGGATGA GGCACAACAG 
GATGCAAGAA ATGAAGCAGC TTTTGAGAAA GAAGTAGTGC TTGATGAAGT GCAATCACAA 
AATGAAGCTC CAGAAGAAAC TTGTTCCAGT GACTCTCTGC AACCTGAACC GTTATTCCAG 
AGAGTAATAC AGCCCCCAGC TGTACCTACA GCCTCACCAG TTCTATCATC TCAGTCTTCC 
CAAGAAGAGC CTTTTGTACC TCGTTCACGT TCCATATCGC CAGCAAAAAA TAAAGGGAAA 
AGTTCATTGT TTTTTGGACT TTCTACAGGT CTGTTTGATG CAAACAACCC AAAGATGTTG 
AGAACGTGTT CACTCCCGGA TCTTTACAAA CTGTACAGAA CCTTAGTTGA TGTTCCCAGC 
ATAGCAGATG TACAGCATCA ACACAGTCTT GAAATAGATG ATACGGAAGA TGAGCAAGCC 
AAAGAAGGAC CCTCTGATTC TGAGGATATC ATGTTTGGGG ACGCCGATAC AGATTTGCAG 
GAACTCCGGG CCTCAATGGA ACAATTACTT AGGGAGCAGC CTAGTGAGGA ATTAAGTGAA 
GAGGAGGAAT CTACTTTAAA GGCTAACGTC ATCAAATGCA TGACAAATGG TACAGAGCTG 
GATGAAGCAG ATGAAAATAA CCCCAGCAGT GAAAGTGCAC TTAATGAAGA ATGGCAGTCA 
GATAATAGTG ATGGAGATAT TGCCAGTGAA TGTGAAGAAT GTGATAGTGT CTTCAGCCAT 
TTGGAAGAGC TGAGATACAA CCTGGAACAG GAAATAGGCT TTGAGAAATT AATTGAAGTT 
TATAATAAAA TAAAGGCCAT ACATGAAGAT GAAGATGAAA ATATTGATAT TTGTTCAAGC 
ATAGTTCAGA CTATTCTGGG AAATGAACAC AAACACTTAT ATGCCAAAGT ACTTCACCTG 
GTCATGGCGG ATGGAGCCTA CCAGGAAGAT AATGACGAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_015285379.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015140865.1 Gallus gallus NIMA-related kinase 1 (NEK1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OGa07768
Clone ID Related Accession (Same CDS sequence)	XM_015285379.1 , XM_015285379.2
Accession Version	XM_015285379.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3618bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	serine/threonine-protein kinase Nek1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455855.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAAAT ACATTAAAGT GCAAAAAATT GGAGAAGGAT CCTTTGGGAA AGCATTTCTT 
GTTAAAGATA AAGAAAATAG CCAGCAGTAT GTTATTAAAG AAATTAACAT ATCTAAGATG 
TCAAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TACTGGCCAA CATGAAACAT 
CCAAATATTG TCCTGTATAG GGAGTCGTTT GAAGAAAATG GTTGTCTCTA CATAGTGATG 
GATTACTGTG AAGGAGGAGA TCTGTTCAAA AAAATAAATG CCCAGAAAGG AGTCCTGTTC 
TCTGAGGATC AGATTCTTGA TTGGTTTGTA CAGATCTGTT TGGCACTGAA ACATATACAT 
GATAGAAAAA TCTTACATCG CGACATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA 
ACAATACAGC TGGGAGATTT TGGAATCGCG AGAGTTCTGA ACAGTACTGT GGAATTGGCT 
CGCACTTGCA TAGGAACACC ATACTATTTA TCACCTGAAA TATGCCAGAA CAGGCCTTAC 
AACAATAAAA GTGATATCTG GGCCCTTGGT TGCGTTCTCT ATGAAATGTG CACACTTAAG 
CATGCATTTG AAGCTGGTAA CATGAAGAAC TTGGTACTGA AGATAATCTC TGGATCTTTC 
CCTCCTGTTT CTGTGCATTA TTCCTATGAC CTACGTAATC TACTGTCACA GTTATTTAAA 
AGGAATCCTA GGGATAGACC ATCAGTGAAC TCCATATTGG AGAAAAATTT CATAGCCAAA 
CGGGTTGAAA AATTTCTCAC ACCGCAGCTT ATTGCAGAAG AATTCAGTCA CAAAGTGTTT 
CACAAGTTTG GACCACATGC TGGACCAGCT AAAAGACCAG CTCAAGGACA TATCTCGGCT 
TCAATTGCAC CAGTGCAGAA AATTACCAAA CCTGCTGCAA AATACGGAGT GCCTTTAGCG 
ATGAAGAAGT GTTATGACGC ACCCAAAAAG CTTCATGAGA AAAAGTCATT GATTAAACTT 
AGGCAGGCCT TTCCAACTCC AAAGAAGAAA ATTATTCCAG GAGAAGAAAG GAGGAAAATG 
TTTGAGGAAC CTTCAAAAAA GAAAAGGTTA GAATTGCCTG AAAAAGAAAA ACGCCAGAGA 
GATCAGATCA GTTTACTGAA GGCAGAGGAA ATGAGAAGAT TGGAAAAAGA AAGGATGGAA 
CGAATAAACA GAGCCAGGGA GCAAGGATGG AGGAATGTGC TTGGTTCAGG TGGAAGTGGT 
GATGTTAAGG CACCATATTA TGGCGGTGGA GGTGGTGTTG GTCCTTTTCC TGCCTCTTTC 
AGAGGACAGT ATGAGCACTA TCATGCTATT TTTGACCAGA TGCAAAAGGA AACCTTCAAA 
GTAACTAAAG AGAGGCAAGC CAAATGGAGG GCGGAGGTAC AAGGCCAAGA AGCTGTTGAG 
AGAGGAATTC CACCTGGAGT ACGTCCAGGA GTTCCTAAGG GGCCTGCAGG TCATCACCAT 
TTACCTGATG CTGATGCAAT TAGGAAAAAA GTGAAAAGGT TGAAGGAGGT GTCTAAACAA 
GCCAGTGCAA ACAGGCAGAA AGGATGGCTA CCTGCAGAAA GAGCAAAGCA AGTTGAAGAA 
TTCTGGCAAC GAAAGAGGGA AGCCATGCAA AACAAAGCTC GTGCTGAGGG ACACATGGAA 
TATTTGGCAA GATTAAGACA GATACGACTA CAAAACTTCA ATGAGCGTCA ACAGATTAAA 
GCAAAACTTC GTGGAGAGAA GAATGAAGCT CCCAGTTCTG ATGGACAAGA GTCAAGTGAG 
GAAGCAGAAA TGAAACGCAA AAAGATAGAA GCACAGAAGG CCCAAGCAAA TGCTCGAGCT 
GCAGTTCTGA AAGAACAGTT AGAGCGAAAG AGGAAGGAGG CATATGAAAG AGAGAAAAGA 
GCTTGGGAAG AACATCTGAT AGCAAAAGGA GTAAAGAATC CTAATGTGCC TTTTCATGTG 
GGAGCAGCAG GACACACTCC TGTGCATGGA ATACAAGAGC CTGGAGTGTC TCCTCCTAAG 
CAACAGCTTC CAGTTAAGCC CATCTCCATG ACTTCTGCTT TGAAAGAAGT TGGCGTGGAT 
GGAAATGTAA CCGCTGCCCA GGAGGTTAAA GAGGAAATAA AAAAGCCAGA TCTTACAATG 
CAGAACAAGA GGCAGATACT GAAAAGATTG AATCAAAATC TTAAAGCTCA GGAAGATGAG 
AAAGGAAGAA AGGACGATAA AAATATCTCT GAGTCAGATG GGTCTGAAGA TGGTAAAGAA 
GAGCAAGAAG ATAACCATCC ACTTCTGGGA GATCGCAAAA GATGGGCATC AAGGGCATCA 
GATTTGGTAG TTCCTTTAGC TCAGTTATCA ATGGAAGATT CTCTCTCTGG AACAGACCGG 
CAAACTATGG GGGAAGTAAT CAGGCTAGAT ATTGGGGAAC CCCGCAGGAA AGTCTGGGGC 
AAAAGCCCTA CTGATTCGGT CCTGAAGATT CTAGGAGAAG CGGAGTTGCA GCTACCAACT 
GAATTACTGG AAGAAATAGA TGTAATAAAT GAGCCTGATG ATTTGGAAGC AGTGATGTTG 
AAAGAAACAG AAGAAGATAA AAAGCACCAG AGCAAGGAAT TTCCCATTGC TGTTAATGAA 
GTGTGGGTGA AAGAAAAACA GGATGAGGCA CAACAGGATG CAAGAAATGA AGCAGCTTTT 
GAGAAAGAAG TAGTGCTTGA TGAAGTGCAA TCACAAAATG AAGCTCCAGA AGAAACTTGT 
TCCAGTGACT CTCTGCAACC TGAACCGTTA TTCCAGAGAG TAATACAGCC CCCAGCTGTA 
CCTACAGCCT CACCAGTTCT ATCATCTCAG TCTTCCCAAG AAGAGCCTTT TGTACCTCGT 
TCACGTTCCA TATCGCCAGC AAAAAATAAA GGGAAAAGTT CATTGTTTTT TGGACTTTCT 
ACAGGTCTGT TTGATGCAAA CAACCCAAAG ATGTTGAGAA CGTGTTCACT CCCGGATCTT 
TACAAACTGT ACAGAACCTT AGTTGATGTT CCCAGCATAG CAGATGTACA GCATCAACAC 
AGTCTTGAAA TAGATGATAC GGAAGATGAG CAAGCCAAAG AAGGACCCTC TGATTCTGAG 
GATATCATGT TTGGGGACGC CGATACAGAT TTGCAGGAAC TCCGGGCCTC AATGGAACAA 
TTACTTAGGG AGCAGCCTAG TGAGGAATTA AGTGAAGAGG AGGAATCTAC TTTAAAGGCT 
AACGTCATCA AATGCATGAC AAATGGTACA GAGCTGGATG AAGCAGATGA AAATAACCCC 
AGCAGTGAAA GTGCACTTAA TGAAGAATGG CAGTCAGATA ATAGTGATGG AGATATTGCC 
AGTGAATGTG AAGAATGTGA TAGTGTCTTC AGCCATTTGG AAGAGCTGAG ATACAACCTG 
GAACAGGAAA TAGGCTTTGA GAAATTAATT GAAGTTTATA ATAAAATAAA GGCCATACAT 
GAAGATGAAG ATGAAAATAT TGATATTTGT TCAAGCATAG TTCAGACTAT TCTGGGAAAT 
GAACACAAAC ACTTATATGC CAAAGTACTT CACCTGGTCA TGGCGGATGG AGCCTACCAG 
GAAGATAATG ACGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015140865.1
CDS	1007..4624
Translation	MDKYIKVQKIGEGSFGKAFLVKDKENSQQYVIKEINISKMSNKEREESRREVAVLANMKHPNIVLYRESFEENGCLYIVMDYCEGGDLFKKINAQKGVLFSEDQILDWFVQICLALKHIHDRKILHRDIKSQNIFLTKDGTIQLGDFGIARVLNSTVELARTCIGTPYYLSPEICQNRPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIISGSFPPVSVHYSYDLRNLLSQLFKRNPRDRPSVNSILEKNFIAKRVEKFLTPQLIAEEFSHKVFHKFGPHAGPAKRPAQGHISASIAPVQKITKPAAKYGVPLAMKKCYDAPKKLHEKKSLIKLRQAFPTPKKKIIPGEERRKMFEEPSKKKRLELPEKEKRQRDQISLLKAEEMRRLEKERMERINRAREQGWRNVLGSGGSGDVKAPYYGGGGGVGPFPASFRGQYEHYHAIFDQMQKETFKVTKERQAKWRAEVQGQEAVERGIPPGVRPGVPKGPAGHHHLPDADAIRKKVKRLKEVSKQASANRQKGWLPAERAKQVEEFWQRKREAMQNKARAEGHMEYLARLRQIRLQNFNERQQIKAKLRGEKNEAPSSDGQESSEEAEMKRKKIEAQKAQANARAAVLKEQLERKRKEAYEREKRAWEEHLIAKGVKNPNVPFHVGAAGHTPVHGIQEPGVSPPKQQLPVKPISMTSALKEVGVDGNVTAAQEVKEEIKKPDLTMQNKRQILKRLNQNLKAQEDEKGRKDDKNISESDGSEDGKEEQEDNHPLLGDRKRWASRASDLVVPLAQLSMEDSLSGTDRQTMGEVIRLDIGEPRRKVWGKSPTDSVLKILGEAELQLPTELLEEIDVINEPDDLEAVMLKETEEDKKHQSKEFPIAVNEVWVKEKQDEAQQDARNEAAFEKEVVLDEVQSQNEAPEETCSSDSLQPEPLFQRVIQPPAVPTASPVLSSQSSQEEPFVPRSRSISPAKNKGKSSLFFGLSTGLFDANNPKMLRTCSLPDLYKLYRTLVDVPSIADVQHQHSLEIDDTEDEQAKEGPSDSEDIMFGDADTDLQELRASMEQLLREQPSEELSEEEESTLKANVIKCMTNGTELDEADENNPSSESALNEEWQSDNSDGDIASECEECDSVFSHLEELRYNLEQEIGFEKLIEVYNKIKAIHEDEDENIDICSSIVQTILGNEHKHLYAKVLHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_015285379.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus NIMA-related kinase 1 (NEK1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015285379.1

ORF Insert Sequence:

ATGGATAAAT ACATTAAAGT GCAAAAAATT GGAGAAGGAT CCTTTGGGAA AGCATTTCTT 
GTTAAAGATA AAGAAAATAG CCAGCAGTAT GTTATTAAAG AAATTAACAT ATCTAAGATG 
TCAAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TACTGGCCAA CATGAAACAT 
CCAAATATTG TCCTGTATAG GGAGTCGTTT GAAGAAAATG GTTGTCTCTA CATAGTGATG 
GATTACTGTG AAGGAGGAGA TCTGTTCAAA AAAATAAATG CCCAGAAAGG AGTCCTGTTC 
TCTGAGGATC AGATTCTTGA TTGGTTTGTA CAGATCTGTT TGGCACTGAA ACATATACAT 
GATAGAAAAA TCTTACATCG CGACATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA 
ACAATACAGC TGGGAGATTT TGGAATCGCG AGAGTTCTGA ACAGTACTGT GGAATTGGCT 
CGCACTTGCA TAGGAACACC ATACTATTTA TCACCTGAAA TATGCCAGAA CAGGCCTTAC 
AACAATAAAA GTGATATCTG GGCCCTTGGT TGCGTTCTCT ATGAAATGTG CACACTTAAG 
CATGCATTTG AAGCTGGTAA CATGAAGAAC TTGGTACTGA AGATAATCTC TGGATCTTTC 
CCTCCTGTTT CTGTGCATTA TTCCTATGAC CTACGTAATC TACTGTCACA GTTATTTAAA 
AGGAATCCTA GGGATAGACC ATCAGTGAAC TCCATATTGG AGAAAAATTT CATAGCCAAA 
CGGGTTGAAA AATTTCTCAC ACCGCAGCTT ATTGCAGAAG AATTCAGTCA CAAAGTGTTT 
CACAAGTTTG GACCACATGC TGGACCAGCT AAAAGACCAG CTCAAGGACA TATCTCGGCT 
TCAATTGCAC CAGTGCAGAA AATTACCAAA CCTGCTGCAA AATACGGAGT GCCTTTAGCG 
ATGAAGAAGT GTTATGACGC ACCCAAAAAG CTTCATGAGA AAAAGTCATT GATTAAACTT 
AGGCAGGCCT TTCCAACTCC AAAGAAGAAA ATTATTCCAG GAGAAGAAAG GAGGAAAATG 
TTTGAGGAAC CTTCAAAAAA GAAAAGGTTA GAATTGCCTG AAAAAGAAAA ACGCCAGAGA 
GATCAGATCA GTTTACTGAA GGCAGAGGAA ATGAGAAGAT TGGAAAAAGA AAGGATGGAA 
CGAATAAACA GAGCCAGGGA GCAAGGATGG AGGAATGTGC TTGGTTCAGG TGGAAGTGGT 
GATGTTAAGG CACCATATTA TGGCGGTGGA GGTGGTGTTG GTCCTTTTCC TGCCTCTTTC 
AGAGGACAGT ATGAGCACTA TCATGCTATT TTTGACCAGA TGCAAAAGGA AACCTTCAAA 
GTAACTAAAG AGAGGCAAGC CAAATGGAGG GCGGAGGTAC AAGGCCAAGA AGCTGTTGAG 
AGAGGAATTC CACCTGGAGT ACGTCCAGGA GTTCCTAAGG GGCCTGCAGG TCATCACCAT 
TTACCTGATG CTGATGCAAT TAGGAAAAAA GTGAAAAGGT TGAAGGAGGT GTCTAAACAA 
GCCAGTGCAA ACAGGCAGAA AGGATGGCTA CCTGCAGAAA GAGCAAAGCA AGTTGAAGAA 
TTCTGGCAAC GAAAGAGGGA AGCCATGCAA AACAAAGCTC GTGCTGAGGG ACACATGGAA 
TATTTGGCAA GATTAAGACA GATACGACTA CAAAACTTCA ATGAGCGTCA ACAGATTAAA 
GCAAAACTTC GTGGAGAGAA GAATGAAGCT CCCAGTTCTG ATGGACAAGA GTCAAGTGAG 
GAAGCAGAAA TGAAACGCAA AAAGATAGAA GCACAGAAGG CCCAAGCAAA TGCTCGAGCT 
GCAGTTCTGA AAGAACAGTT AGAGCGAAAG AGGAAGGAGG CATATGAAAG AGAGAAAAGA 
GCTTGGGAAG AACATCTGAT AGCAAAAGGA GTAAAGAATC CTAATGTGCC TTTTCATGTG 
GGAGCAGCAG GACACACTCC TGTGCATGGA ATACAAGAGC CTGGAGTGTC TCCTCCTAAG 
CAACAGCTTC CAGTTAAGCC CATCTCCATG ACTTCTGCTT TGAAAGAAGT TGGCGTGGAT 
GGAAATGTAA CCGCTGCCCA GGAGGTTAAA GAGGAAATAA AAAAGCCAGA TCTTACAATG 
CAGAACAAGA GGCAGATACT GAAAAGATTG AATCAAAATC TTAAAGCTCA GGAAGATGAG 
AAAGGAAGAA AGGACGATAA AAATATCTCT GAGTCAGATG GGTCTGAAGA TGGTAAAGAA 
GAGCAAGAAG ATAACCATCC ACTTCTGGGA GATCGCAAAA GATGGGCATC AAGGGCATCA 
GATTTGGTAG TTCCTTTAGC TCAGTTATCA ATGGAAGATT CTCTCTCTGG AACAGACCGG 
CAAACTATGG GGGAAGTAAT CAGGCTAGAT ATTGGGGAAC CCCGCAGGAA AGTCTGGGGC 
AAAAGCCCTA CTGATTCGGT CCTGAAGATT CTAGGAGAAG CGGAGTTGCA GCTACCAACT 
GAATTACTGG AAGAAATAGA TGTAATAAAT GAGCCTGATG ATTTGGAAGC AGTGATGTTG 
AAAGAAACAG AAGAAGATAA AAAGCACCAG AGCAAGGAAT TTCCCATTGC TGTTAATGAA 
GTGTGGGTGA AAGAAAAACA GGATGAGGCA CAACAGGATG CAAGAAATGA AGCAGCTTTT 
GAGAAAGAAG TAGTGCTTGA TGAAGTGCAA TCACAAAATG AAGCTCCAGA AGAAACTTGT 
TCCAGTGACT CTCTGCAACC TGAACCGTTA TTCCAGAGAG TAATACAGCC CCCAGCTGTA 
CCTACAGCCT CACCAGTTCT ATCATCTCAG TCTTCCCAAG AAGAGCCTTT TGTACCTCGT 
TCACGTTCCA TATCGCCAGC AAAAAATAAA GGGAAAAGTT CATTGTTTTT TGGACTTTCT 
ACAGGTCTGT TTGATGCAAA CAACCCAAAG ATGTTGAGAA CGTGTTCACT CCCGGATCTT 
TACAAACTGT ACAGAACCTT AGTTGATGTT CCCAGCATAG CAGATGTACA GCATCAACAC 
AGTCTTGAAA TAGATGATAC GGAAGATGAG CAAGCCAAAG AAGGACCCTC TGATTCTGAG 
GATATCATGT TTGGGGACGC CGATACAGAT TTGCAGGAAC TCCGGGCCTC AATGGAACAA 
TTACTTAGGG AGCAGCCTAG TGAGGAATTA AGTGAAGAGG AGGAATCTAC TTTAAAGGCT 
AACGTCATCA AATGCATGAC AAATGGTACA GAGCTGGATG AAGCAGATGA AAATAACCCC 
AGCAGTGAAA GTGCACTTAA TGAAGAATGG CAGTCAGATA ATAGTGATGG AGATATTGCC 
AGTGAATGTG AAGAATGTGA TAGTGTCTTC AGCCATTTGG AAGAGCTGAG ATACAACCTG 
GAACAGGAAA TAGGCTTTGA GAAATTAATT GAAGTTTATA ATAAAATAAA GGCCATACAT 
GAAGATGAAG ATGAAAATAT TGATATTTGT TCAAGCATAG TTCAGACTAT TCTGGGAAAT 
GAACACAAAC ACTTATATGC CAAAGTACTT CACCTGGTCA TGGCGGATGG AGCCTACCAG 
GAAGATAATG ACGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_015285379.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015140865.1 Gallus gallus NIMA related kinase 1 (NEK1), transcript variant X6, mRNA.

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CloneID	OGa07768
Clone ID Related Accession (Same CDS sequence)	XM_015285379.1 , XM_015285379.2
Accession Version	XM_015285379.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3618bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	serine/threonine-protein kinase Nek1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015285379.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAAAT ACATTAAAGT GCAAAAAATT GGAGAAGGAT CCTTTGGGAA AGCATTTCTT 
GTTAAAGATA AAGAAAATAG CCAGCAGTAT GTTATTAAAG AAATTAACAT ATCTAAGATG 
TCAAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TACTGGCCAA CATGAAACAT 
CCAAATATTG TCCTGTATAG GGAGTCGTTT GAAGAAAATG GTTGTCTCTA CATAGTGATG 
GATTACTGTG AAGGAGGAGA TCTGTTCAAA AAAATAAATG CCCAGAAAGG AGTCCTGTTC 
TCTGAGGATC AGATTCTTGA TTGGTTTGTA CAGATCTGTT TGGCACTGAA ACATATACAT 
GATAGAAAAA TCTTACATCG CGACATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA 
ACAATACAGC TGGGAGATTT TGGAATCGCG AGAGTTCTGA ACAGTACTGT GGAATTGGCT 
CGCACTTGCA TAGGAACACC ATACTATTTA TCACCTGAAA TATGCCAGAA CAGGCCTTAC 
AACAATAAAA GTGATATCTG GGCCCTTGGT TGCGTTCTCT ATGAAATGTG CACACTTAAG 
CATGCATTTG AAGCTGGTAA CATGAAGAAC TTGGTACTGA AGATAATCTC TGGATCTTTC 
CCTCCTGTTT CTGTGCATTA TTCCTATGAC CTACGTAATC TACTGTCACA GTTATTTAAA 
AGGAATCCTA GGGATAGACC ATCAGTGAAC TCCATATTGG AGAAAAATTT CATAGCCAAA 
CGGGTTGAAA AATTTCTCAC ACCGCAGCTT ATTGCAGAAG AATTCAGTCA CAAAGTGTTT 
CACAAGTTTG GACCACATGC TGGACCAGCT AAAAGACCAG CTCAAGGACA TATCTCGGCT 
TCAATTGCAC CAGTGCAGAA AATTACCAAA CCTGCTGCAA AATACGGAGT GCCTTTAGCG 
ATGAAGAAGT GTTATGACGC ACCCAAAAAG CTTCATGAGA AAAAGTCATT GATTAAACTT 
AGGCAGGCCT TTCCAACTCC AAAGAAGAAA ATTATTCCAG GAGAAGAAAG GAGGAAAATG 
TTTGAGGAAC CTTCAAAAAA GAAAAGGTTA GAATTGCCTG AAAAAGAAAA ACGCCAGAGA 
GATCAGATCA GTTTACTGAA GGCAGAGGAA ATGAGAAGAT TGGAAAAAGA AAGGATGGAA 
CGAATAAACA GAGCCAGGGA GCAAGGATGG AGGAATGTGC TTGGTTCAGG TGGAAGTGGT 
GATGTTAAGG CACCATATTA TGGCGGTGGA GGTGGTGTTG GTCCTTTTCC TGCCTCTTTC 
AGAGGACAGT ATGAGCACTA TCATGCTATT TTTGACCAGA TGCAAAAGGA AACCTTCAAA 
GTAACTAAAG AGAGGCAAGC CAAATGGAGG GCGGAGGTAC AAGGCCAAGA AGCTGTTGAG 
AGAGGAATTC CACCTGGAGT ACGTCCAGGA GTTCCTAAGG GGCCTGCAGG TCATCACCAT 
TTACCTGATG CTGATGCAAT TAGGAAAAAA GTGAAAAGGT TGAAGGAGGT GTCTAAACAA 
GCCAGTGCAA ACAGGCAGAA AGGATGGCTA CCTGCAGAAA GAGCAAAGCA AGTTGAAGAA 
TTCTGGCAAC GAAAGAGGGA AGCCATGCAA AACAAAGCTC GTGCTGAGGG ACACATGGAA 
TATTTGGCAA GATTAAGACA GATACGACTA CAAAACTTCA ATGAGCGTCA ACAGATTAAA 
GCAAAACTTC GTGGAGAGAA GAATGAAGCT CCCAGTTCTG ATGGACAAGA GTCAAGTGAG 
GAAGCAGAAA TGAAACGCAA AAAGATAGAA GCACAGAAGG CCCAAGCAAA TGCTCGAGCT 
GCAGTTCTGA AAGAACAGTT AGAGCGAAAG AGGAAGGAGG CATATGAAAG AGAGAAAAGA 
GCTTGGGAAG AACATCTGAT AGCAAAAGGA GTAAAGAATC CTAATGTGCC TTTTCATGTG 
GGAGCAGCAG GACACACTCC TGTGCATGGA ATACAAGAGC CTGGAGTGTC TCCTCCTAAG 
CAACAGCTTC CAGTTAAGCC CATCTCCATG ACTTCTGCTT TGAAAGAAGT TGGCGTGGAT 
GGAAATGTAA CCGCTGCCCA GGAGGTTAAA GAGGAAATAA AAAAGCCAGA TCTTACAATG 
CAGAACAAGA GGCAGATACT GAAAAGATTG AATCAAAATC TTAAAGCTCA GGAAGATGAG 
AAAGGAAGAA AGGACGATAA AAATATCTCT GAGTCAGATG GGTCTGAAGA TGGTAAAGAA 
GAGCAAGAAG ATAACCATCC ACTTCTGGGA GATCGCAAAA GATGGGCATC AAGGGCATCA 
GATTTGGTAG TTCCTTTAGC TCAGTTATCA ATGGAAGATT CTCTCTCTGG AACAGACCGG 
CAAACTATGG GGGAAGTAAT CAGGCTAGAT ATTGGGGAAC CCCGCAGGAA AGTCTGGGGC 
AAAAGCCCTA CTGATTCGGT CCTGAAGATT CTAGGAGAAG CGGAGTTGCA GCTACCAACT 
GAATTACTGG AAGAAATAGA TGTAATAAAT GAGCCTGATG ATTTGGAAGC AGTGATGTTG 
AAAGAAACAG AAGAAGATAA AAAGCACCAG AGCAAGGAAT TTCCCATTGC TGTTAATGAA 
GTGTGGGTGA AAGAAAAACA GGATGAGGCA CAACAGGATG CAAGAAATGA AGCAGCTTTT 
GAGAAAGAAG TAGTGCTTGA TGAAGTGCAA TCACAAAATG AAGCTCCAGA AGAAACTTGT 
TCCAGTGACT CTCTGCAACC TGAACCGTTA TTCCAGAGAG TAATACAGCC CCCAGCTGTA 
CCTACAGCCT CACCAGTTCT ATCATCTCAG TCTTCCCAAG AAGAGCCTTT TGTACCTCGT 
TCACGTTCCA TATCGCCAGC AAAAAATAAA GGGAAAAGTT CATTGTTTTT TGGACTTTCT 
ACAGGTCTGT TTGATGCAAA CAACCCAAAG ATGTTGAGAA CGTGTTCACT CCCGGATCTT 
TACAAACTGT ACAGAACCTT AGTTGATGTT CCCAGCATAG CAGATGTACA GCATCAACAC 
AGTCTTGAAA TAGATGATAC GGAAGATGAG CAAGCCAAAG AAGGACCCTC TGATTCTGAG 
GATATCATGT TTGGGGACGC CGATACAGAT TTGCAGGAAC TCCGGGCCTC AATGGAACAA 
TTACTTAGGG AGCAGCCTAG TGAGGAATTA AGTGAAGAGG AGGAATCTAC TTTAAAGGCT 
AACGTCATCA AATGCATGAC AAATGGTACA GAGCTGGATG AAGCAGATGA AAATAACCCC 
AGCAGTGAAA GTGCACTTAA TGAAGAATGG CAGTCAGATA ATAGTGATGG AGATATTGCC 
AGTGAATGTG AAGAATGTGA TAGTGTCTTC AGCCATTTGG AAGAGCTGAG ATACAACCTG 
GAACAGGAAA TAGGCTTTGA GAAATTAATT GAAGTTTATA ATAAAATAAA GGCCATACAT 
GAAGATGAAG ATGAAAATAT TGATATTTGT TCAAGCATAG TTCAGACTAT TCTGGGAAAT 
GAACACAAAC ACTTATATGC CAAAGTACTT CACCTGGTCA TGGCGGATGG AGCCTACCAG 
GAAGATAATG ACGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015140865.1
CDS	1004..4621
Translation	MDKYIKVQKIGEGSFGKAFLVKDKENSQQYVIKEINISKMSNKEREESRREVAVLANMKHPNIVLYRESFEENGCLYIVMDYCEGGDLFKKINAQKGVLFSEDQILDWFVQICLALKHIHDRKILHRDIKSQNIFLTKDGTIQLGDFGIARVLNSTVELARTCIGTPYYLSPEICQNRPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIISGSFPPVSVHYSYDLRNLLSQLFKRNPRDRPSVNSILEKNFIAKRVEKFLTPQLIAEEFSHKVFHKFGPHAGPAKRPAQGHISASIAPVQKITKPAAKYGVPLAMKKCYDAPKKLHEKKSLIKLRQAFPTPKKKIIPGEERRKMFEEPSKKKRLELPEKEKRQRDQISLLKAEEMRRLEKERMERINRAREQGWRNVLGSGGSGDVKAPYYGGGGGVGPFPASFRGQYEHYHAIFDQMQKETFKVTKERQAKWRAEVQGQEAVERGIPPGVRPGVPKGPAGHHHLPDADAIRKKVKRLKEVSKQASANRQKGWLPAERAKQVEEFWQRKREAMQNKARAEGHMEYLARLRQIRLQNFNERQQIKAKLRGEKNEAPSSDGQESSEEAEMKRKKIEAQKAQANARAAVLKEQLERKRKEAYEREKRAWEEHLIAKGVKNPNVPFHVGAAGHTPVHGIQEPGVSPPKQQLPVKPISMTSALKEVGVDGNVTAAQEVKEEIKKPDLTMQNKRQILKRLNQNLKAQEDEKGRKDDKNISESDGSEDGKEEQEDNHPLLGDRKRWASRASDLVVPLAQLSMEDSLSGTDRQTMGEVIRLDIGEPRRKVWGKSPTDSVLKILGEAELQLPTELLEEIDVINEPDDLEAVMLKETEEDKKHQSKEFPIAVNEVWVKEKQDEAQQDARNEAAFEKEVVLDEVQSQNEAPEETCSSDSLQPEPLFQRVIQPPAVPTASPVLSSQSSQEEPFVPRSRSISPAKNKGKSSLFFGLSTGLFDANNPKMLRTCSLPDLYKLYRTLVDVPSIADVQHQHSLEIDDTEDEQAKEGPSDSEDIMFGDADTDLQELRASMEQLLREQPSEELSEEEESTLKANVIKCMTNGTELDEADENNPSSESALNEEWQSDNSDGDIASECEECDSVFSHLEELRYNLEQEIGFEKLIEVYNKIKAIHEDEDENIDICSSIVQTILGNEHKHLYAKVLHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_015285379.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus NIMA related kinase 1 (NEK1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015285379.2

ORF Insert Sequence:

ATGGATAAAT ACATTAAAGT GCAAAAAATT GGAGAAGGAT CCTTTGGGAA AGCATTTCTT 
GTTAAAGATA AAGAAAATAG CCAGCAGTAT GTTATTAAAG AAATTAACAT ATCTAAGATG 
TCAAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TACTGGCCAA CATGAAACAT 
CCAAATATTG TCCTGTATAG GGAGTCGTTT GAAGAAAATG GTTGTCTCTA CATAGTGATG 
GATTACTGTG AAGGAGGAGA TCTGTTCAAA AAAATAAATG CCCAGAAAGG AGTCCTGTTC 
TCTGAGGATC AGATTCTTGA TTGGTTTGTA CAGATCTGTT TGGCACTGAA ACATATACAT 
GATAGAAAAA TCTTACATCG CGACATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA 
ACAATACAGC TGGGAGATTT TGGAATCGCG AGAGTTCTGA ACAGTACTGT GGAATTGGCT 
CGCACTTGCA TAGGAACACC ATACTATTTA TCACCTGAAA TATGCCAGAA CAGGCCTTAC 
AACAATAAAA GTGATATCTG GGCCCTTGGT TGCGTTCTCT ATGAAATGTG CACACTTAAG 
CATGCATTTG AAGCTGGTAA CATGAAGAAC TTGGTACTGA AGATAATCTC TGGATCTTTC 
CCTCCTGTTT CTGTGCATTA TTCCTATGAC CTACGTAATC TACTGTCACA GTTATTTAAA 
AGGAATCCTA GGGATAGACC ATCAGTGAAC TCCATATTGG AGAAAAATTT CATAGCCAAA 
CGGGTTGAAA AATTTCTCAC ACCGCAGCTT ATTGCAGAAG AATTCAGTCA CAAAGTGTTT 
CACAAGTTTG GACCACATGC TGGACCAGCT AAAAGACCAG CTCAAGGACA TATCTCGGCT 
TCAATTGCAC CAGTGCAGAA AATTACCAAA CCTGCTGCAA AATACGGAGT GCCTTTAGCG 
ATGAAGAAGT GTTATGACGC ACCCAAAAAG CTTCATGAGA AAAAGTCATT GATTAAACTT 
AGGCAGGCCT TTCCAACTCC AAAGAAGAAA ATTATTCCAG GAGAAGAAAG GAGGAAAATG 
TTTGAGGAAC CTTCAAAAAA GAAAAGGTTA GAATTGCCTG AAAAAGAAAA ACGCCAGAGA 
GATCAGATCA GTTTACTGAA GGCAGAGGAA ATGAGAAGAT TGGAAAAAGA AAGGATGGAA 
CGAATAAACA GAGCCAGGGA GCAAGGATGG AGGAATGTGC TTGGTTCAGG TGGAAGTGGT 
GATGTTAAGG CACCATATTA TGGCGGTGGA GGTGGTGTTG GTCCTTTTCC TGCCTCTTTC 
AGAGGACAGT ATGAGCACTA TCATGCTATT TTTGACCAGA TGCAAAAGGA AACCTTCAAA 
GTAACTAAAG AGAGGCAAGC CAAATGGAGG GCGGAGGTAC AAGGCCAAGA AGCTGTTGAG 
AGAGGAATTC CACCTGGAGT ACGTCCAGGA GTTCCTAAGG GGCCTGCAGG TCATCACCAT 
TTACCTGATG CTGATGCAAT TAGGAAAAAA GTGAAAAGGT TGAAGGAGGT GTCTAAACAA 
GCCAGTGCAA ACAGGCAGAA AGGATGGCTA CCTGCAGAAA GAGCAAAGCA AGTTGAAGAA 
TTCTGGCAAC GAAAGAGGGA AGCCATGCAA AACAAAGCTC GTGCTGAGGG ACACATGGAA 
TATTTGGCAA GATTAAGACA GATACGACTA CAAAACTTCA ATGAGCGTCA ACAGATTAAA 
GCAAAACTTC GTGGAGAGAA GAATGAAGCT CCCAGTTCTG ATGGACAAGA GTCAAGTGAG 
GAAGCAGAAA TGAAACGCAA AAAGATAGAA GCACAGAAGG CCCAAGCAAA TGCTCGAGCT 
GCAGTTCTGA AAGAACAGTT AGAGCGAAAG AGGAAGGAGG CATATGAAAG AGAGAAAAGA 
GCTTGGGAAG AACATCTGAT AGCAAAAGGA GTAAAGAATC CTAATGTGCC TTTTCATGTG 
GGAGCAGCAG GACACACTCC TGTGCATGGA ATACAAGAGC CTGGAGTGTC TCCTCCTAAG 
CAACAGCTTC CAGTTAAGCC CATCTCCATG ACTTCTGCTT TGAAAGAAGT TGGCGTGGAT 
GGAAATGTAA CCGCTGCCCA GGAGGTTAAA GAGGAAATAA AAAAGCCAGA TCTTACAATG 
CAGAACAAGA GGCAGATACT GAAAAGATTG AATCAAAATC TTAAAGCTCA GGAAGATGAG 
AAAGGAAGAA AGGACGATAA AAATATCTCT GAGTCAGATG GGTCTGAAGA TGGTAAAGAA 
GAGCAAGAAG ATAACCATCC ACTTCTGGGA GATCGCAAAA GATGGGCATC AAGGGCATCA 
GATTTGGTAG TTCCTTTAGC TCAGTTATCA ATGGAAGATT CTCTCTCTGG AACAGACCGG 
CAAACTATGG GGGAAGTAAT CAGGCTAGAT ATTGGGGAAC CCCGCAGGAA AGTCTGGGGC 
AAAAGCCCTA CTGATTCGGT CCTGAAGATT CTAGGAGAAG CGGAGTTGCA GCTACCAACT 
GAATTACTGG AAGAAATAGA TGTAATAAAT GAGCCTGATG ATTTGGAAGC AGTGATGTTG 
AAAGAAACAG AAGAAGATAA AAAGCACCAG AGCAAGGAAT TTCCCATTGC TGTTAATGAA 
GTGTGGGTGA AAGAAAAACA GGATGAGGCA CAACAGGATG CAAGAAATGA AGCAGCTTTT 
GAGAAAGAAG TAGTGCTTGA TGAAGTGCAA TCACAAAATG AAGCTCCAGA AGAAACTTGT 
TCCAGTGACT CTCTGCAACC TGAACCGTTA TTCCAGAGAG TAATACAGCC CCCAGCTGTA 
CCTACAGCCT CACCAGTTCT ATCATCTCAG TCTTCCCAAG AAGAGCCTTT TGTACCTCGT 
TCACGTTCCA TATCGCCAGC AAAAAATAAA GGGAAAAGTT CATTGTTTTT TGGACTTTCT 
ACAGGTCTGT TTGATGCAAA CAACCCAAAG ATGTTGAGAA CGTGTTCACT CCCGGATCTT 
TACAAACTGT ACAGAACCTT AGTTGATGTT CCCAGCATAG CAGATGTACA GCATCAACAC 
AGTCTTGAAA TAGATGATAC GGAAGATGAG CAAGCCAAAG AAGGACCCTC TGATTCTGAG 
GATATCATGT TTGGGGACGC CGATACAGAT TTGCAGGAAC TCCGGGCCTC AATGGAACAA 
TTACTTAGGG AGCAGCCTAG TGAGGAATTA AGTGAAGAGG AGGAATCTAC TTTAAAGGCT 
AACGTCATCA AATGCATGAC AAATGGTACA GAGCTGGATG AAGCAGATGA AAATAACCCC 
AGCAGTGAAA GTGCACTTAA TGAAGAATGG CAGTCAGATA ATAGTGATGG AGATATTGCC 
AGTGAATGTG AAGAATGTGA TAGTGTCTTC AGCCATTTGG AAGAGCTGAG ATACAACCTG 
GAACAGGAAA TAGGCTTTGA GAAATTAATT GAAGTTTATA ATAAAATAAA GGCCATACAT 
GAAGATGAAG ATGAAAATAT TGATATTTGT TCAAGCATAG TTCAGACTAT TCTGGGAAAT 
GAACACAAAC ACTTATATGC CAAAGTACTT CACCTGGTCA TGGCGGATGG AGCCTACCAG 
GAAGATAATG ACGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_015285381.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015140867.1 Gallus gallus NIMA related kinase 1 (NEK1), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OGa07769
Clone ID Related Accession (Same CDS sequence)	XM_015285381.2 , XM_015285381.1
Accession Version	XM_015285381.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3570bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	serine/threonine-protein kinase Nek1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015285381.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAAAT ACATTAAAGT GCAAAAAATT GGAGAAGGAT CCTTTGGGAA AGCATTTCTT 
GTTAAAGATA AAGAAAATAG CCAGCAGTAT GTTATTAAAG AAATTAACAT ATCTAAGATG 
TCAAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TACTGGCCAA CATGAAACAT 
CCAAATATTG TCCTGTATAG GGAGTCGTTT GAAGAAAATG GTTGTCTCTA CATAGTGATG 
GATTACTGTG AAGGAGGAGA TCTGTTCAAA AAAATAAATG CCCAGAAAGG AGTCCTGTTC 
TCTGAGGATC AGATTCTTGA TTGGTTTGTA CAGATCTGTT TGGCACTGAA ACATATACAT 
GATAGAAAAA TCTTACATCG CGACATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA 
ACAATACAGC TGGGAGATTT TGGAATCGCG AGAGTTCTGA ACAGTACTGT GGAATTGGCT 
CGCACTTGCA TAGGAACACC ATACTATTTA TCACCTGAAA TATGCCAGAA CAGGCCTTAC 
AACAATAAAA GTGATATCTG GGCCCTTGGT TGCGTTCTCT ATGAAATGTG CACACTTAAG 
CATGCATTTG AAGCTGGTAA CATGAAGAAC TTGGTACTGA AGATAATCTC TGGATCTTTC 
CCTCCTGTTT CTGTGCATTA TTCCTATGAC CTACGTAATC TACTGTCACA GTTATTTAAA 
AGGAATCCTA GGGATAGACC ATCAGTGAAC TCCATATTGG AGAAAAATTT CATAGCCAAA 
CGGGTTGAAA AATTTCTCAC ACCGCAGCTT ATTGCAGAAG AATTCAGTCA CAAAGTGTTT 
CACAAGTTTG GACCACATGC TGGACCAGCT AAAAGACCAG CTCAAGGACA TATCTCGGCT 
TCAATTGCAC CAGTGCAGAA AATTACCAAA CCTGCTGCAA AATACGGAGT GCCTTTAGCG 
ATGAAGAAGT GTTATGACGC ACCCAAAAAG CTTCATGAGA AAAAGTCATT GATTAAACTT 
AGGCAGGCCT TTCCAACTCC AAAGAAGAAA ATTATTCCAG GAGAAGAAAG GAGGAAAATG 
TTTGAGGAAC CTTCAAAAAA GAAAAGGTTA GAATTGCCTG AAAAAGAAAA ACGCCAGAGA 
GATCAGATCA GTTTACTGAA GGCAGAGGAA ATGAGAAGAT TGGAAAAAGA AAGGATGGAA 
CGAATAAACA GAGCCAGGGA GCAAGGATGG AGGAATGTGC TTGGTTCAGG TGGAAGTGGT 
GATGTTAAGG CACCATATTA TGGCGGTGGA GGTGGTGTTG GTCCTTTTCC TGCCTCTTTC 
AGAGGACAGT ATGAGCACTA TCATGCTATT TTTGACCAGA TGCAAAAGGA AACCTTCAAA 
GTAACTAAAG AGAGGCAAGC CAAATGGAGG GCGGAGGTAC AAGGCCAAGA AGCTGTTGAG 
AGGCAGAAAG GATGGCTACC TGCAGAAAGA GCAAAGCAAG TTGAAGAATT CTGGCAACGA 
AAGAGGGAAG CCATGCAAAA CAAAGCTCGT GCTGAGGGAC ACATGGGTAC ACTGCAAAAC 
GTGGCAGATA TCTATGGAGG CAGGCCCATG TCTGCAAGAG GAAGGAAATC CAGAAACAAG 
GAAGAAGAGG AATATTTGGC AAGATTAAGA CAGATACGAC TACAAAACTT CAATGAGCGT 
CAACAGATTA AAGCAAAACT TCGTGGAGAG AAGAATGAAG CTCCCAGTTC TGATGGACAA 
GAGTCAAGTG AGGAAGCAGA AATGAAACGC AAAAAGATAG AAGCACAGAA GGCCCAAGCA 
AATGCTCGAG CTGCAGTTCT GAAAGAACAG TTAGAGCGAA AGAGGAAGGA GGCATATGAA 
AGAGAGAAAA GAGCTTGGGA AGAACATCTG ATAGCAAAAG GAGTAAAGAA TCCTAATGTG 
CCTTTTCATG TGGGAGCAGC AGGACACACT CCTGTGCATG GAATACAAGA GCCTGGAGTG 
TCTCCTCCTA AGCAACAGCT TCCAGTTAAG CCCATCTCCA TGACTTCTGC TTTGAAAGAA 
GTTGGCGTGG ATGGAAATGT AACCGCTGCC CAGGAGGTTA AAGAGGAAAT AAAAAAGCCA 
GATCTTACAA TGCAGAACAA GAGGCAGATA CTGAAAAGAT TGAATCAAAA TCTTAAAGCT 
CAGGAAGATG AGAAAGGAAG AAAGGACGAT AAAAATATCT CTGAGTCAGA TGGGTCTGAA 
GATGGTAAAG AAGAGCAAGA AGATAACCAT CCACTTCTGG GAGATCGCAA AAGATGGGCA 
TCAAGGGCAT CAGATTTGGT AGTTCCTTTA GCTCAGTTAT CAATGGAAGA TTCTCTCTCT 
GGAACAGACC GGCAAACTAT GGGGGAAGTA ATCAGGCTAG ATATTGGGGA ACCCCGCAGG 
AAAGTCTGGG GCAAAAGCCC TACTGATTCG GTCCTGAAGA TTCTAGGAGA AGCGGAGTTG 
CAGCTACCAA CTGAATTACT GGAAGAAATA GATGTAATAA ATGAGCCTGA TGATTTGGAA 
GCAGTGATGT TGAAAGAAAC AGAAGAAGAT AAAAAGCACC AGAGCAAGGA ATTTCCCATT 
GCTGTTAATG AAGTGTGGGT GAAAGAAAAA CAGGATGAGG CACAACAGGA TGCAAGAAAT 
GAAGCAGCTT TTGAGAAAGA AGTAGTGCTT GATGAAGTGC AATCACAAAA TGAAGCTCCA 
GAAGAAACTT GTTCCAGTGA CTCTCTGCAA CCTGAACCGT TATTCCAGAG AGTAATACAG 
CCCCCAGCTG TACCTACAGC CTCACCAGTT CTATCATCTC AGTCTTCCCA AGAAGAGCCT 
TTTGTACCTC GTTCACGTTC CATATCGCCA GCAAAAAATA AAGGGAAAAG TTCATTGTTT 
TTTGGACTTT CTACAGGTCT GTTTGATGCA AACAACCCAA AGATGTTGAG AACGTGTTCA 
CTCCCGGATC TTTACAAACT GTACAGAACC TTAGTTGATG TTCCCAGCAT AGCAGATGTA 
CAGCATCAAC ACAGTCTTGA AATAGATGAT ACGGAAGATG AGCAAGCCAA AGAAGGACCC 
TCTGATTCTG AGGATATCAT GTTTGGGGAC GCCGATACAG ATTTGCAGGA ACTCCGGGCC 
TCAATGGAAC AATTACTTAG GGAGCAGCCT AGTGAGGAAT TAAGTGAAGA GGAGGAATCT 
ACTTTAAAGG CTAACGTCAT CAAATGCATG ACAAATGGTA CAGAGCTGGA TGAAGCAGAT 
GAAAATAACC CCAGCAGTGA AAGTGCACTT AATGAAGAAT GGCAGTCAGA TAATAGTGAT 
GGAGATATTG CCAGTGAATG TGAAGAATGT GATAGTGTCT TCAGCCATTT GGAAGAGCTG 
AGATACAACC TGGAACAGGA AATAGGCTTT GAGAAATTAA TTGAAGTTTA TAATAAAATA 
AAGGCCATAC ATGAAGATGA AGATGAAAAT ATTGATATTT GTTCAAGCAT AGTTCAGACT 
ATTCTGGGAA ATGAACACAA ACACTTATAT GCCAAAGTAC TTCACCTGGT CATGGCGGAT 
GGAGCCTACC AGGAAGATAA TGACGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015140867.1
CDS	1004..4573
Translation	MDKYIKVQKIGEGSFGKAFLVKDKENSQQYVIKEINISKMSNKEREESRREVAVLANMKHPNIVLYRESFEENGCLYIVMDYCEGGDLFKKINAQKGVLFSEDQILDWFVQICLALKHIHDRKILHRDIKSQNIFLTKDGTIQLGDFGIARVLNSTVELARTCIGTPYYLSPEICQNRPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIISGSFPPVSVHYSYDLRNLLSQLFKRNPRDRPSVNSILEKNFIAKRVEKFLTPQLIAEEFSHKVFHKFGPHAGPAKRPAQGHISASIAPVQKITKPAAKYGVPLAMKKCYDAPKKLHEKKSLIKLRQAFPTPKKKIIPGEERRKMFEEPSKKKRLELPEKEKRQRDQISLLKAEEMRRLEKERMERINRAREQGWRNVLGSGGSGDVKAPYYGGGGGVGPFPASFRGQYEHYHAIFDQMQKETFKVTKERQAKWRAEVQGQEAVERQKGWLPAERAKQVEEFWQRKREAMQNKARAEGHMGTLQNVADIYGGRPMSARGRKSRNKEEEEYLARLRQIRLQNFNERQQIKAKLRGEKNEAPSSDGQESSEEAEMKRKKIEAQKAQANARAAVLKEQLERKRKEAYEREKRAWEEHLIAKGVKNPNVPFHVGAAGHTPVHGIQEPGVSPPKQQLPVKPISMTSALKEVGVDGNVTAAQEVKEEIKKPDLTMQNKRQILKRLNQNLKAQEDEKGRKDDKNISESDGSEDGKEEQEDNHPLLGDRKRWASRASDLVVPLAQLSMEDSLSGTDRQTMGEVIRLDIGEPRRKVWGKSPTDSVLKILGEAELQLPTELLEEIDVINEPDDLEAVMLKETEEDKKHQSKEFPIAVNEVWVKEKQDEAQQDARNEAAFEKEVVLDEVQSQNEAPEETCSSDSLQPEPLFQRVIQPPAVPTASPVLSSQSSQEEPFVPRSRSISPAKNKGKSSLFFGLSTGLFDANNPKMLRTCSLPDLYKLYRTLVDVPSIADVQHQHSLEIDDTEDEQAKEGPSDSEDIMFGDADTDLQELRASMEQLLREQPSEELSEEEESTLKANVIKCMTNGTELDEADENNPSSESALNEEWQSDNSDGDIASECEECDSVFSHLEELRYNLEQEIGFEKLIEVYNKIKAIHEDEDENIDICSSIVQTILGNEHKHLYAKVLHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_015285381.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus NIMA related kinase 1 (NEK1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015285381.2

ORF Insert Sequence:

ATGGATAAAT ACATTAAAGT GCAAAAAATT GGAGAAGGAT CCTTTGGGAA AGCATTTCTT 
GTTAAAGATA AAGAAAATAG CCAGCAGTAT GTTATTAAAG AAATTAACAT ATCTAAGATG 
TCAAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TACTGGCCAA CATGAAACAT 
CCAAATATTG TCCTGTATAG GGAGTCGTTT GAAGAAAATG GTTGTCTCTA CATAGTGATG 
GATTACTGTG AAGGAGGAGA TCTGTTCAAA AAAATAAATG CCCAGAAAGG AGTCCTGTTC 
TCTGAGGATC AGATTCTTGA TTGGTTTGTA CAGATCTGTT TGGCACTGAA ACATATACAT 
GATAGAAAAA TCTTACATCG CGACATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA 
ACAATACAGC TGGGAGATTT TGGAATCGCG AGAGTTCTGA ACAGTACTGT GGAATTGGCT 
CGCACTTGCA TAGGAACACC ATACTATTTA TCACCTGAAA TATGCCAGAA CAGGCCTTAC 
AACAATAAAA GTGATATCTG GGCCCTTGGT TGCGTTCTCT ATGAAATGTG CACACTTAAG 
CATGCATTTG AAGCTGGTAA CATGAAGAAC TTGGTACTGA AGATAATCTC TGGATCTTTC 
CCTCCTGTTT CTGTGCATTA TTCCTATGAC CTACGTAATC TACTGTCACA GTTATTTAAA 
AGGAATCCTA GGGATAGACC ATCAGTGAAC TCCATATTGG AGAAAAATTT CATAGCCAAA 
CGGGTTGAAA AATTTCTCAC ACCGCAGCTT ATTGCAGAAG AATTCAGTCA CAAAGTGTTT 
CACAAGTTTG GACCACATGC TGGACCAGCT AAAAGACCAG CTCAAGGACA TATCTCGGCT 
TCAATTGCAC CAGTGCAGAA AATTACCAAA CCTGCTGCAA AATACGGAGT GCCTTTAGCG 
ATGAAGAAGT GTTATGACGC ACCCAAAAAG CTTCATGAGA AAAAGTCATT GATTAAACTT 
AGGCAGGCCT TTCCAACTCC AAAGAAGAAA ATTATTCCAG GAGAAGAAAG GAGGAAAATG 
TTTGAGGAAC CTTCAAAAAA GAAAAGGTTA GAATTGCCTG AAAAAGAAAA ACGCCAGAGA 
GATCAGATCA GTTTACTGAA GGCAGAGGAA ATGAGAAGAT TGGAAAAAGA AAGGATGGAA 
CGAATAAACA GAGCCAGGGA GCAAGGATGG AGGAATGTGC TTGGTTCAGG TGGAAGTGGT 
GATGTTAAGG CACCATATTA TGGCGGTGGA GGTGGTGTTG GTCCTTTTCC TGCCTCTTTC 
AGAGGACAGT ATGAGCACTA TCATGCTATT TTTGACCAGA TGCAAAAGGA AACCTTCAAA 
GTAACTAAAG AGAGGCAAGC CAAATGGAGG GCGGAGGTAC AAGGCCAAGA AGCTGTTGAG 
AGGCAGAAAG GATGGCTACC TGCAGAAAGA GCAAAGCAAG TTGAAGAATT CTGGCAACGA 
AAGAGGGAAG CCATGCAAAA CAAAGCTCGT GCTGAGGGAC ACATGGGTAC ACTGCAAAAC 
GTGGCAGATA TCTATGGAGG CAGGCCCATG TCTGCAAGAG GAAGGAAATC CAGAAACAAG 
GAAGAAGAGG AATATTTGGC AAGATTAAGA CAGATACGAC TACAAAACTT CAATGAGCGT 
CAACAGATTA AAGCAAAACT TCGTGGAGAG AAGAATGAAG CTCCCAGTTC TGATGGACAA 
GAGTCAAGTG AGGAAGCAGA AATGAAACGC AAAAAGATAG AAGCACAGAA GGCCCAAGCA 
AATGCTCGAG CTGCAGTTCT GAAAGAACAG TTAGAGCGAA AGAGGAAGGA GGCATATGAA 
AGAGAGAAAA GAGCTTGGGA AGAACATCTG ATAGCAAAAG GAGTAAAGAA TCCTAATGTG 
CCTTTTCATG TGGGAGCAGC AGGACACACT CCTGTGCATG GAATACAAGA GCCTGGAGTG 
TCTCCTCCTA AGCAACAGCT TCCAGTTAAG CCCATCTCCA TGACTTCTGC TTTGAAAGAA 
GTTGGCGTGG ATGGAAATGT AACCGCTGCC CAGGAGGTTA AAGAGGAAAT AAAAAAGCCA 
GATCTTACAA TGCAGAACAA GAGGCAGATA CTGAAAAGAT TGAATCAAAA TCTTAAAGCT 
CAGGAAGATG AGAAAGGAAG AAAGGACGAT AAAAATATCT CTGAGTCAGA TGGGTCTGAA 
GATGGTAAAG AAGAGCAAGA AGATAACCAT CCACTTCTGG GAGATCGCAA AAGATGGGCA 
TCAAGGGCAT CAGATTTGGT AGTTCCTTTA GCTCAGTTAT CAATGGAAGA TTCTCTCTCT 
GGAACAGACC GGCAAACTAT GGGGGAAGTA ATCAGGCTAG ATATTGGGGA ACCCCGCAGG 
AAAGTCTGGG GCAAAAGCCC TACTGATTCG GTCCTGAAGA TTCTAGGAGA AGCGGAGTTG 
CAGCTACCAA CTGAATTACT GGAAGAAATA GATGTAATAA ATGAGCCTGA TGATTTGGAA 
GCAGTGATGT TGAAAGAAAC AGAAGAAGAT AAAAAGCACC AGAGCAAGGA ATTTCCCATT 
GCTGTTAATG AAGTGTGGGT GAAAGAAAAA CAGGATGAGG CACAACAGGA TGCAAGAAAT 
GAAGCAGCTT TTGAGAAAGA AGTAGTGCTT GATGAAGTGC AATCACAAAA TGAAGCTCCA 
GAAGAAACTT GTTCCAGTGA CTCTCTGCAA CCTGAACCGT TATTCCAGAG AGTAATACAG 
CCCCCAGCTG TACCTACAGC CTCACCAGTT CTATCATCTC AGTCTTCCCA AGAAGAGCCT 
TTTGTACCTC GTTCACGTTC CATATCGCCA GCAAAAAATA AAGGGAAAAG TTCATTGTTT 
TTTGGACTTT CTACAGGTCT GTTTGATGCA AACAACCCAA AGATGTTGAG AACGTGTTCA 
CTCCCGGATC TTTACAAACT GTACAGAACC TTAGTTGATG TTCCCAGCAT AGCAGATGTA 
CAGCATCAAC ACAGTCTTGA AATAGATGAT ACGGAAGATG AGCAAGCCAA AGAAGGACCC 
TCTGATTCTG AGGATATCAT GTTTGGGGAC GCCGATACAG ATTTGCAGGA ACTCCGGGCC 
TCAATGGAAC AATTACTTAG GGAGCAGCCT AGTGAGGAAT TAAGTGAAGA GGAGGAATCT 
ACTTTAAAGG CTAACGTCAT CAAATGCATG ACAAATGGTA CAGAGCTGGA TGAAGCAGAT 
GAAAATAACC CCAGCAGTGA AAGTGCACTT AATGAAGAAT GGCAGTCAGA TAATAGTGAT 
GGAGATATTG CCAGTGAATG TGAAGAATGT GATAGTGTCT TCAGCCATTT GGAAGAGCTG 
AGATACAACC TGGAACAGGA AATAGGCTTT GAGAAATTAA TTGAAGTTTA TAATAAAATA 
AAGGCCATAC ATGAAGATGA AGATGAAAAT ATTGATATTT GTTCAAGCAT AGTTCAGACT 
ATTCTGGGAA ATGAACACAA ACACTTATAT GCCAAAGTAC TTCACCTGGT CATGGCGGAT 
GGAGCCTACC AGGAAGATAA TGACGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_015285381.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015140867.1 Gallus gallus NIMA-related kinase 1 (NEK1), transcript variant X8, mRNA.

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CloneID	OGa07769
Clone ID Related Accession (Same CDS sequence)	XM_015285381.2 , XM_015285381.1
Accession Version	XM_015285381.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3570bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	serine/threonine-protein kinase Nek1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455855.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAAAT ACATTAAAGT GCAAAAAATT GGAGAAGGAT CCTTTGGGAA AGCATTTCTT 
GTTAAAGATA AAGAAAATAG CCAGCAGTAT GTTATTAAAG AAATTAACAT ATCTAAGATG 
TCAAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TACTGGCCAA CATGAAACAT 
CCAAATATTG TCCTGTATAG GGAGTCGTTT GAAGAAAATG GTTGTCTCTA CATAGTGATG 
GATTACTGTG AAGGAGGAGA TCTGTTCAAA AAAATAAATG CCCAGAAAGG AGTCCTGTTC 
TCTGAGGATC AGATTCTTGA TTGGTTTGTA CAGATCTGTT TGGCACTGAA ACATATACAT 
GATAGAAAAA TCTTACATCG CGACATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA 
ACAATACAGC TGGGAGATTT TGGAATCGCG AGAGTTCTGA ACAGTACTGT GGAATTGGCT 
CGCACTTGCA TAGGAACACC ATACTATTTA TCACCTGAAA TATGCCAGAA CAGGCCTTAC 
AACAATAAAA GTGATATCTG GGCCCTTGGT TGCGTTCTCT ATGAAATGTG CACACTTAAG 
CATGCATTTG AAGCTGGTAA CATGAAGAAC TTGGTACTGA AGATAATCTC TGGATCTTTC 
CCTCCTGTTT CTGTGCATTA TTCCTATGAC CTACGTAATC TACTGTCACA GTTATTTAAA 
AGGAATCCTA GGGATAGACC ATCAGTGAAC TCCATATTGG AGAAAAATTT CATAGCCAAA 
CGGGTTGAAA AATTTCTCAC ACCGCAGCTT ATTGCAGAAG AATTCAGTCA CAAAGTGTTT 
CACAAGTTTG GACCACATGC TGGACCAGCT AAAAGACCAG CTCAAGGACA TATCTCGGCT 
TCAATTGCAC CAGTGCAGAA AATTACCAAA CCTGCTGCAA AATACGGAGT GCCTTTAGCG 
ATGAAGAAGT GTTATGACGC ACCCAAAAAG CTTCATGAGA AAAAGTCATT GATTAAACTT 
AGGCAGGCCT TTCCAACTCC AAAGAAGAAA ATTATTCCAG GAGAAGAAAG GAGGAAAATG 
TTTGAGGAAC CTTCAAAAAA GAAAAGGTTA GAATTGCCTG AAAAAGAAAA ACGCCAGAGA 
GATCAGATCA GTTTACTGAA GGCAGAGGAA ATGAGAAGAT TGGAAAAAGA AAGGATGGAA 
CGAATAAACA GAGCCAGGGA GCAAGGATGG AGGAATGTGC TTGGTTCAGG TGGAAGTGGT 
GATGTTAAGG CACCATATTA TGGCGGTGGA GGTGGTGTTG GTCCTTTTCC TGCCTCTTTC 
AGAGGACAGT ATGAGCACTA TCATGCTATT TTTGACCAGA TGCAAAAGGA AACCTTCAAA 
GTAACTAAAG AGAGGCAAGC CAAATGGAGG GCGGAGGTAC AAGGCCAAGA AGCTGTTGAG 
AGGCAGAAAG GATGGCTACC TGCAGAAAGA GCAAAGCAAG TTGAAGAATT CTGGCAACGA 
AAGAGGGAAG CCATGCAAAA CAAAGCTCGT GCTGAGGGAC ACATGGGTAC ACTGCAAAAC 
GTGGCAGATA TCTATGGAGG CAGGCCCATG TCTGCAAGAG GAAGGAAATC CAGAAACAAG 
GAAGAAGAGG AATATTTGGC AAGATTAAGA CAGATACGAC TACAAAACTT CAATGAGCGT 
CAACAGATTA AAGCAAAACT TCGTGGAGAG AAGAATGAAG CTCCCAGTTC TGATGGACAA 
GAGTCAAGTG AGGAAGCAGA AATGAAACGC AAAAAGATAG AAGCACAGAA GGCCCAAGCA 
AATGCTCGAG CTGCAGTTCT GAAAGAACAG TTAGAGCGAA AGAGGAAGGA GGCATATGAA 
AGAGAGAAAA GAGCTTGGGA AGAACATCTG ATAGCAAAAG GAGTAAAGAA TCCTAATGTG 
CCTTTTCATG TGGGAGCAGC AGGACACACT CCTGTGCATG GAATACAAGA GCCTGGAGTG 
TCTCCTCCTA AGCAACAGCT TCCAGTTAAG CCCATCTCCA TGACTTCTGC TTTGAAAGAA 
GTTGGCGTGG ATGGAAATGT AACCGCTGCC CAGGAGGTTA AAGAGGAAAT AAAAAAGCCA 
GATCTTACAA TGCAGAACAA GAGGCAGATA CTGAAAAGAT TGAATCAAAA TCTTAAAGCT 
CAGGAAGATG AGAAAGGAAG AAAGGACGAT AAAAATATCT CTGAGTCAGA TGGGTCTGAA 
GATGGTAAAG AAGAGCAAGA AGATAACCAT CCACTTCTGG GAGATCGCAA AAGATGGGCA 
TCAAGGGCAT CAGATTTGGT AGTTCCTTTA GCTCAGTTAT CAATGGAAGA TTCTCTCTCT 
GGAACAGACC GGCAAACTAT GGGGGAAGTA ATCAGGCTAG ATATTGGGGA ACCCCGCAGG 
AAAGTCTGGG GCAAAAGCCC TACTGATTCG GTCCTGAAGA TTCTAGGAGA AGCGGAGTTG 
CAGCTACCAA CTGAATTACT GGAAGAAATA GATGTAATAA ATGAGCCTGA TGATTTGGAA 
GCAGTGATGT TGAAAGAAAC AGAAGAAGAT AAAAAGCACC AGAGCAAGGA ATTTCCCATT 
GCTGTTAATG AAGTGTGGGT GAAAGAAAAA CAGGATGAGG CACAACAGGA TGCAAGAAAT 
GAAGCAGCTT TTGAGAAAGA AGTAGTGCTT GATGAAGTGC AATCACAAAA TGAAGCTCCA 
GAAGAAACTT GTTCCAGTGA CTCTCTGCAA CCTGAACCGT TATTCCAGAG AGTAATACAG 
CCCCCAGCTG TACCTACAGC CTCACCAGTT CTATCATCTC AGTCTTCCCA AGAAGAGCCT 
TTTGTACCTC GTTCACGTTC CATATCGCCA GCAAAAAATA AAGGGAAAAG TTCATTGTTT 
TTTGGACTTT CTACAGGTCT GTTTGATGCA AACAACCCAA AGATGTTGAG AACGTGTTCA 
CTCCCGGATC TTTACAAACT GTACAGAACC TTAGTTGATG TTCCCAGCAT AGCAGATGTA 
CAGCATCAAC ACAGTCTTGA AATAGATGAT ACGGAAGATG AGCAAGCCAA AGAAGGACCC 
TCTGATTCTG AGGATATCAT GTTTGGGGAC GCCGATACAG ATTTGCAGGA ACTCCGGGCC 
TCAATGGAAC AATTACTTAG GGAGCAGCCT AGTGAGGAAT TAAGTGAAGA GGAGGAATCT 
ACTTTAAAGG CTAACGTCAT CAAATGCATG ACAAATGGTA CAGAGCTGGA TGAAGCAGAT 
GAAAATAACC CCAGCAGTGA AAGTGCACTT AATGAAGAAT GGCAGTCAGA TAATAGTGAT 
GGAGATATTG CCAGTGAATG TGAAGAATGT GATAGTGTCT TCAGCCATTT GGAAGAGCTG 
AGATACAACC TGGAACAGGA AATAGGCTTT GAGAAATTAA TTGAAGTTTA TAATAAAATA 
AAGGCCATAC ATGAAGATGA AGATGAAAAT ATTGATATTT GTTCAAGCAT AGTTCAGACT 
ATTCTGGGAA ATGAACACAA ACACTTATAT GCCAAAGTAC TTCACCTGGT CATGGCGGAT 
GGAGCCTACC AGGAAGATAA TGACGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015140867.1
CDS	1006..4575
Translation	MDKYIKVQKIGEGSFGKAFLVKDKENSQQYVIKEINISKMSNKEREESRREVAVLANMKHPNIVLYRESFEENGCLYIVMDYCEGGDLFKKINAQKGVLFSEDQILDWFVQICLALKHIHDRKILHRDIKSQNIFLTKDGTIQLGDFGIARVLNSTVELARTCIGTPYYLSPEICQNRPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIISGSFPPVSVHYSYDLRNLLSQLFKRNPRDRPSVNSILEKNFIAKRVEKFLTPQLIAEEFSHKVFHKFGPHAGPAKRPAQGHISASIAPVQKITKPAAKYGVPLAMKKCYDAPKKLHEKKSLIKLRQAFPTPKKKIIPGEERRKMFEEPSKKKRLELPEKEKRQRDQISLLKAEEMRRLEKERMERINRAREQGWRNVLGSGGSGDVKAPYYGGGGGVGPFPASFRGQYEHYHAIFDQMQKETFKVTKERQAKWRAEVQGQEAVERQKGWLPAERAKQVEEFWQRKREAMQNKARAEGHMGTLQNVADIYGGRPMSARGRKSRNKEEEEYLARLRQIRLQNFNERQQIKAKLRGEKNEAPSSDGQESSEEAEMKRKKIEAQKAQANARAAVLKEQLERKRKEAYEREKRAWEEHLIAKGVKNPNVPFHVGAAGHTPVHGIQEPGVSPPKQQLPVKPISMTSALKEVGVDGNVTAAQEVKEEIKKPDLTMQNKRQILKRLNQNLKAQEDEKGRKDDKNISESDGSEDGKEEQEDNHPLLGDRKRWASRASDLVVPLAQLSMEDSLSGTDRQTMGEVIRLDIGEPRRKVWGKSPTDSVLKILGEAELQLPTELLEEIDVINEPDDLEAVMLKETEEDKKHQSKEFPIAVNEVWVKEKQDEAQQDARNEAAFEKEVVLDEVQSQNEAPEETCSSDSLQPEPLFQRVIQPPAVPTASPVLSSQSSQEEPFVPRSRSISPAKNKGKSSLFFGLSTGLFDANNPKMLRTCSLPDLYKLYRTLVDVPSIADVQHQHSLEIDDTEDEQAKEGPSDSEDIMFGDADTDLQELRASMEQLLREQPSEELSEEEESTLKANVIKCMTNGTELDEADENNPSSESALNEEWQSDNSDGDIASECEECDSVFSHLEELRYNLEQEIGFEKLIEVYNKIKAIHEDEDENIDICSSIVQTILGNEHKHLYAKVLHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_015285381.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus NIMA-related kinase 1 (NEK1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015285381.1

ORF Insert Sequence:

ATGGATAAAT ACATTAAAGT GCAAAAAATT GGAGAAGGAT CCTTTGGGAA AGCATTTCTT 
GTTAAAGATA AAGAAAATAG CCAGCAGTAT GTTATTAAAG AAATTAACAT ATCTAAGATG 
TCAAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TACTGGCCAA CATGAAACAT 
CCAAATATTG TCCTGTATAG GGAGTCGTTT GAAGAAAATG GTTGTCTCTA CATAGTGATG 
GATTACTGTG AAGGAGGAGA TCTGTTCAAA AAAATAAATG CCCAGAAAGG AGTCCTGTTC 
TCTGAGGATC AGATTCTTGA TTGGTTTGTA CAGATCTGTT TGGCACTGAA ACATATACAT 
GATAGAAAAA TCTTACATCG CGACATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA 
ACAATACAGC TGGGAGATTT TGGAATCGCG AGAGTTCTGA ACAGTACTGT GGAATTGGCT 
CGCACTTGCA TAGGAACACC ATACTATTTA TCACCTGAAA TATGCCAGAA CAGGCCTTAC 
AACAATAAAA GTGATATCTG GGCCCTTGGT TGCGTTCTCT ATGAAATGTG CACACTTAAG 
CATGCATTTG AAGCTGGTAA CATGAAGAAC TTGGTACTGA AGATAATCTC TGGATCTTTC 
CCTCCTGTTT CTGTGCATTA TTCCTATGAC CTACGTAATC TACTGTCACA GTTATTTAAA 
AGGAATCCTA GGGATAGACC ATCAGTGAAC TCCATATTGG AGAAAAATTT CATAGCCAAA 
CGGGTTGAAA AATTTCTCAC ACCGCAGCTT ATTGCAGAAG AATTCAGTCA CAAAGTGTTT 
CACAAGTTTG GACCACATGC TGGACCAGCT AAAAGACCAG CTCAAGGACA TATCTCGGCT 
TCAATTGCAC CAGTGCAGAA AATTACCAAA CCTGCTGCAA AATACGGAGT GCCTTTAGCG 
ATGAAGAAGT GTTATGACGC ACCCAAAAAG CTTCATGAGA AAAAGTCATT GATTAAACTT 
AGGCAGGCCT TTCCAACTCC AAAGAAGAAA ATTATTCCAG GAGAAGAAAG GAGGAAAATG 
TTTGAGGAAC CTTCAAAAAA GAAAAGGTTA GAATTGCCTG AAAAAGAAAA ACGCCAGAGA 
GATCAGATCA GTTTACTGAA GGCAGAGGAA ATGAGAAGAT TGGAAAAAGA AAGGATGGAA 
CGAATAAACA GAGCCAGGGA GCAAGGATGG AGGAATGTGC TTGGTTCAGG TGGAAGTGGT 
GATGTTAAGG CACCATATTA TGGCGGTGGA GGTGGTGTTG GTCCTTTTCC TGCCTCTTTC 
AGAGGACAGT ATGAGCACTA TCATGCTATT TTTGACCAGA TGCAAAAGGA AACCTTCAAA 
GTAACTAAAG AGAGGCAAGC CAAATGGAGG GCGGAGGTAC AAGGCCAAGA AGCTGTTGAG 
AGGCAGAAAG GATGGCTACC TGCAGAAAGA GCAAAGCAAG TTGAAGAATT CTGGCAACGA 
AAGAGGGAAG CCATGCAAAA CAAAGCTCGT GCTGAGGGAC ACATGGGTAC ACTGCAAAAC 
GTGGCAGATA TCTATGGAGG CAGGCCCATG TCTGCAAGAG GAAGGAAATC CAGAAACAAG 
GAAGAAGAGG AATATTTGGC AAGATTAAGA CAGATACGAC TACAAAACTT CAATGAGCGT 
CAACAGATTA AAGCAAAACT TCGTGGAGAG AAGAATGAAG CTCCCAGTTC TGATGGACAA 
GAGTCAAGTG AGGAAGCAGA AATGAAACGC AAAAAGATAG AAGCACAGAA GGCCCAAGCA 
AATGCTCGAG CTGCAGTTCT GAAAGAACAG TTAGAGCGAA AGAGGAAGGA GGCATATGAA 
AGAGAGAAAA GAGCTTGGGA AGAACATCTG ATAGCAAAAG GAGTAAAGAA TCCTAATGTG 
CCTTTTCATG TGGGAGCAGC AGGACACACT CCTGTGCATG GAATACAAGA GCCTGGAGTG 
TCTCCTCCTA AGCAACAGCT TCCAGTTAAG CCCATCTCCA TGACTTCTGC TTTGAAAGAA 
GTTGGCGTGG ATGGAAATGT AACCGCTGCC CAGGAGGTTA AAGAGGAAAT AAAAAAGCCA 
GATCTTACAA TGCAGAACAA GAGGCAGATA CTGAAAAGAT TGAATCAAAA TCTTAAAGCT 
CAGGAAGATG AGAAAGGAAG AAAGGACGAT AAAAATATCT CTGAGTCAGA TGGGTCTGAA 
GATGGTAAAG AAGAGCAAGA AGATAACCAT CCACTTCTGG GAGATCGCAA AAGATGGGCA 
TCAAGGGCAT CAGATTTGGT AGTTCCTTTA GCTCAGTTAT CAATGGAAGA TTCTCTCTCT 
GGAACAGACC GGCAAACTAT GGGGGAAGTA ATCAGGCTAG ATATTGGGGA ACCCCGCAGG 
AAAGTCTGGG GCAAAAGCCC TACTGATTCG GTCCTGAAGA TTCTAGGAGA AGCGGAGTTG 
CAGCTACCAA CTGAATTACT GGAAGAAATA GATGTAATAA ATGAGCCTGA TGATTTGGAA 
GCAGTGATGT TGAAAGAAAC AGAAGAAGAT AAAAAGCACC AGAGCAAGGA ATTTCCCATT 
GCTGTTAATG AAGTGTGGGT GAAAGAAAAA CAGGATGAGG CACAACAGGA TGCAAGAAAT 
GAAGCAGCTT TTGAGAAAGA AGTAGTGCTT GATGAAGTGC AATCACAAAA TGAAGCTCCA 
GAAGAAACTT GTTCCAGTGA CTCTCTGCAA CCTGAACCGT TATTCCAGAG AGTAATACAG 
CCCCCAGCTG TACCTACAGC CTCACCAGTT CTATCATCTC AGTCTTCCCA AGAAGAGCCT 
TTTGTACCTC GTTCACGTTC CATATCGCCA GCAAAAAATA AAGGGAAAAG TTCATTGTTT 
TTTGGACTTT CTACAGGTCT GTTTGATGCA AACAACCCAA AGATGTTGAG AACGTGTTCA 
CTCCCGGATC TTTACAAACT GTACAGAACC TTAGTTGATG TTCCCAGCAT AGCAGATGTA 
CAGCATCAAC ACAGTCTTGA AATAGATGAT ACGGAAGATG AGCAAGCCAA AGAAGGACCC 
TCTGATTCTG AGGATATCAT GTTTGGGGAC GCCGATACAG ATTTGCAGGA ACTCCGGGCC 
TCAATGGAAC AATTACTTAG GGAGCAGCCT AGTGAGGAAT TAAGTGAAGA GGAGGAATCT 
ACTTTAAAGG CTAACGTCAT CAAATGCATG ACAAATGGTA CAGAGCTGGA TGAAGCAGAT 
GAAAATAACC CCAGCAGTGA AAGTGCACTT AATGAAGAAT GGCAGTCAGA TAATAGTGAT 
GGAGATATTG CCAGTGAATG TGAAGAATGT GATAGTGTCT TCAGCCATTT GGAAGAGCTG 
AGATACAACC TGGAACAGGA AATAGGCTTT GAGAAATTAA TTGAAGTTTA TAATAAAATA 
AAGGCCATAC ATGAAGATGA AGATGAAAAT ATTGATATTT GTTCAAGCAT AGTTCAGACT 
ATTCTGGGAA ATGAACACAA ACACTTATAT GCCAAAGTAC TTCACCTGGT CATGGCGGAT 
GGAGCCTACC AGGAAGATAA TGACGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_015285382.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015140868.1 Gallus gallus NIMA-related kinase 1 (NEK1), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OGa07770
Clone ID Related Accession (Same CDS sequence)	XM_015285382.1 , XM_015285382.2
Accession Version	XM_015285382.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3486bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	serine/threonine-protein kinase Nek1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455855.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAAAT ACATTAAAGT GCAAAAAATT GGAGAAGGAT CCTTTGGGAA AGCATTTCTT 
GTTAAAGATA AAGAAAATAG CCAGCAGTAT GTTATTAAAG AAATTAACAT ATCTAAGATG 
TCAAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TACTGGCCAA CATGAAACAT 
CCAAATATTG TCCTGTATAG GGAGTCGTTT GAAGAAAATG GTTGTCTCTA CATAGTGATG 
GATTACTGTG AAGGAGGAGA TCTGTTCAAA AAAATAAATG CCCAGAAAGG AGTCCTGTTC 
TCTGAGGATC AGATTCTTGA TTGGTTTGTA CAGATCTGTT TGGCACTGAA ACATATACAT 
GATAGAAAAA TCTTACATCG CGACATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA 
ACAATACAGC TGGGAGATTT TGGAATCGCG AGAGTTCTGA ACAGTACTGT GGAATTGGCT 
CGCACTTGCA TAGGAACACC ATACTATTTA TCACCTGAAA TATGCCAGAA CAGGCCTTAC 
AACAATAAAA GTGATATCTG GGCCCTTGGT TGCGTTCTCT ATGAAATGTG CACACTTAAG 
CATGCATTTG AAGCTGGTAA CATGAAGAAC TTGGTACTGA AGATAATCTC TGGATCTTTC 
CCTCCTGTTT CTGTGCATTA TTCCTATGAC CTACGTAATC TACTGTCACA GTTATTTAAA 
AGGAATCCTA GGGATAGACC ATCAGTGAAC TCCATATTGG AGAAAAATTT CATAGCCAAA 
CGGGTTGAAA AATTTCTCAC ACCGCAGCTT ATTGCAGAAG AATTCAGTCA CAAAGTGTTT 
CACAAGTTTG GACCACATGC TGGACCAGCT AAAAGACCAG CTCAAGGACA TATCTCGGCT 
TCAATTGCAC CAGTGCAGAA AATTACCAAA CCTGCTGCAA AATACGGAGT GCCTTTAGCG 
ATGAAGAAGT GTTATGACGC ACCCAAAAAG CTTCATGAGA AAAAGTCATT GATTAAACTT 
AGGCAGGCCT TTCCAACTCC AAAGAAGAAA ATTATTCCAG GAGAAGAAAG GAGGAAAATG 
TTTGAGGAAC CTTCAAAAAA GAAAAGGTTA GAATTGCCTG AAAAAGAAAA ACGCCAGAGA 
GATCAGATCA GTTTACTGAA GGCAGAGGAA ATGAGAAGAT TGGAAAAAGA AAGGATGGAA 
CGAATAAACA GAGCCAGGGA GCAAGGATGG AGGAATGTGC TTGGTTCAGG TGGAAGTGGT 
GATGTTAAGG CACCATATTA TGGCGGTGGA GGTGGTGTTG GTCCTTTTCC TGCCTCTTTC 
AGAGGACAGT ATGAGCACTA TCATGCTATT TTTGACCAGA TGCAAAAGGA AACCTTCAAA 
GTAACTAAAG AGAGGCAAGC CAAATGGAGG GCGGAGGTAC AAGGCCAAGA AGCTGTTGAG 
AGGCAGAAAG GATGGCTACC TGCAGAAAGA GCAAAGCAAG TTGAAGAATT CTGGCAACGA 
AAGAGGGAAG CCATGCAAAA CAAAGCTCGT GCTGAGGGAC ACATGGAATA TTTGGCAAGA 
TTAAGACAGA TACGACTACA AAACTTCAAT GAGCGTCAAC AGATTAAAGC AAAACTTCGT 
GGAGAGAAGA ATGAAGCTCC CAGTTCTGAT GGACAAGAGT CAAGTGAGGA AGCAGAAATG 
AAACGCAAAA AGATAGAAGC ACAGAAGGCC CAAGCAAATG CTCGAGCTGC AGTTCTGAAA 
GAACAGTTAG AGCGAAAGAG GAAGGAGGCA TATGAAAGAG AGAAAAGAGC TTGGGAAGAA 
CATCTGATAG CAAAAGGAGT AAAGAATCCT AATGTGCCTT TTCATGTGGG AGCAGCAGGA 
CACACTCCTG TGCATGGAAT ACAAGAGCCT GGAGTGTCTC CTCCTAAGCA ACAGCTTCCA 
GTTAAGCCCA TCTCCATGAC TTCTGCTTTG AAAGAAGTTG GCGTGGATGG AAATGTAACC 
GCTGCCCAGG AGGTTAAAGA GGAAATAAAA AAGCCAGATC TTACAATGCA GAACAAGAGG 
CAGATACTGA AAAGATTGAA TCAAAATCTT AAAGCTCAGG AAGATGAGAA AGGAAGAAAG 
GACGATAAAA ATATCTCTGA GTCAGATGGG TCTGAAGATG GTAAAGAAGA GCAAGAAGAT 
AACCATCCAC TTCTGGGAGA TCGCAAAAGA TGGGCATCAA GGGCATCAGA TTTGGTAGTT 
CCTTTAGCTC AGTTATCAAT GGAAGATTCT CTCTCTGGAA CAGACCGGCA AACTATGGGG 
GAAGTAATCA GGCTAGATAT TGGGGAACCC CGCAGGAAAG TCTGGGGCAA AAGCCCTACT 
GATTCGGTCC TGAAGATTCT AGGAGAAGCG GAGTTGCAGC TACCAACTGA ATTACTGGAA 
GAAATAGATG TAATAAATGA GCCTGATGAT TTGGAAGCAG TGATGTTGAA AGAAACAGAA 
GAAGATAAAA AGCACCAGAG CAAGGAATTT CCCATTGCTG TTAATGAAGT GTGGGTGAAA 
GAAAAACAGG ATGAGGCACA ACAGGATGCA AGAAATGAAG CAGCTTTTGA GAAAGAAGTA 
GTGCTTGATG AAGTGCAATC ACAAAATGAA GCTCCAGAAG AAACTTGTTC CAGTGACTCT 
CTGCAACCTG AACCGTTATT CCAGAGAGTA ATACAGCCCC CAGCTGTACC TACAGCCTCA 
CCAGTTCTAT CATCTCAGTC TTCCCAAGAA GAGCCTTTTG TACCTCGTTC ACGTTCCATA 
TCGCCAGCAA AAAATAAAGG GAAAAGTTCA TTGTTTTTTG GACTTTCTAC AGGTCTGTTT 
GATGCAAACA ACCCAAAGAT GTTGAGAACG TGTTCACTCC CGGATCTTTA CAAACTGTAC 
AGAACCTTAG TTGATGTTCC CAGCATAGCA GATGTACAGC ATCAACACAG TCTTGAAATA 
GATGATACGG AAGATGAGCA AGCCAAAGAA GGACCCTCTG ATTCTGAGGA TATCATGTTT 
GGGGACGCCG ATACAGATTT GCAGGAACTC CGGGCCTCAA TGGAACAATT ACTTAGGGAG 
CAGCCTAGTG AGGAATTAAG TGAAGAGGAG GAATCTACTT TAAAGGCTAA CGTCATCAAA 
TGCATGACAA ATGGTACAGA GCTGGATGAA GCAGATGAAA ATAACCCCAG CAGTGAAAGT 
GCACTTAATG AAGAATGGCA GTCAGATAAT AGTGATGGAG ATATTGCCAG TGAATGTGAA 
GAATGTGATA GTGTCTTCAG CCATTTGGAA GAGCTGAGAT ACAACCTGGA ACAGGAAATA 
GGCTTTGAGA AATTAATTGA AGTTTATAAT AAAATAAAGG CCATACATGA AGATGAAGAT 
GAAAATATTG ATATTTGTTC AAGCATAGTT CAGACTATTC TGGGAAATGA ACACAAACAC 
TTATATGCCA AAGTACTTCA CCTGGTCATG GCGGATGGAG CCTACCAGGA AGATAATGAC 
GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015140868.1
CDS	1006..4491
Translation	MDKYIKVQKIGEGSFGKAFLVKDKENSQQYVIKEINISKMSNKEREESRREVAVLANMKHPNIVLYRESFEENGCLYIVMDYCEGGDLFKKINAQKGVLFSEDQILDWFVQICLALKHIHDRKILHRDIKSQNIFLTKDGTIQLGDFGIARVLNSTVELARTCIGTPYYLSPEICQNRPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIISGSFPPVSVHYSYDLRNLLSQLFKRNPRDRPSVNSILEKNFIAKRVEKFLTPQLIAEEFSHKVFHKFGPHAGPAKRPAQGHISASIAPVQKITKPAAKYGVPLAMKKCYDAPKKLHEKKSLIKLRQAFPTPKKKIIPGEERRKMFEEPSKKKRLELPEKEKRQRDQISLLKAEEMRRLEKERMERINRAREQGWRNVLGSGGSGDVKAPYYGGGGGVGPFPASFRGQYEHYHAIFDQMQKETFKVTKERQAKWRAEVQGQEAVERQKGWLPAERAKQVEEFWQRKREAMQNKARAEGHMEYLARLRQIRLQNFNERQQIKAKLRGEKNEAPSSDGQESSEEAEMKRKKIEAQKAQANARAAVLKEQLERKRKEAYEREKRAWEEHLIAKGVKNPNVPFHVGAAGHTPVHGIQEPGVSPPKQQLPVKPISMTSALKEVGVDGNVTAAQEVKEEIKKPDLTMQNKRQILKRLNQNLKAQEDEKGRKDDKNISESDGSEDGKEEQEDNHPLLGDRKRWASRASDLVVPLAQLSMEDSLSGTDRQTMGEVIRLDIGEPRRKVWGKSPTDSVLKILGEAELQLPTELLEEIDVINEPDDLEAVMLKETEEDKKHQSKEFPIAVNEVWVKEKQDEAQQDARNEAAFEKEVVLDEVQSQNEAPEETCSSDSLQPEPLFQRVIQPPAVPTASPVLSSQSSQEEPFVPRSRSISPAKNKGKSSLFFGLSTGLFDANNPKMLRTCSLPDLYKLYRTLVDVPSIADVQHQHSLEIDDTEDEQAKEGPSDSEDIMFGDADTDLQELRASMEQLLREQPSEELSEEEESTLKANVIKCMTNGTELDEADENNPSSESALNEEWQSDNSDGDIASECEECDSVFSHLEELRYNLEQEIGFEKLIEVYNKIKAIHEDEDENIDICSSIVQTILGNEHKHLYAKVLHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_015285382.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus NIMA-related kinase 1 (NEK1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015285382.1

ORF Insert Sequence:

ATGGATAAAT ACATTAAAGT GCAAAAAATT GGAGAAGGAT CCTTTGGGAA AGCATTTCTT 
GTTAAAGATA AAGAAAATAG CCAGCAGTAT GTTATTAAAG AAATTAACAT ATCTAAGATG 
TCAAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TACTGGCCAA CATGAAACAT 
CCAAATATTG TCCTGTATAG GGAGTCGTTT GAAGAAAATG GTTGTCTCTA CATAGTGATG 
GATTACTGTG AAGGAGGAGA TCTGTTCAAA AAAATAAATG CCCAGAAAGG AGTCCTGTTC 
TCTGAGGATC AGATTCTTGA TTGGTTTGTA CAGATCTGTT TGGCACTGAA ACATATACAT 
GATAGAAAAA TCTTACATCG CGACATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA 
ACAATACAGC TGGGAGATTT TGGAATCGCG AGAGTTCTGA ACAGTACTGT GGAATTGGCT 
CGCACTTGCA TAGGAACACC ATACTATTTA TCACCTGAAA TATGCCAGAA CAGGCCTTAC 
AACAATAAAA GTGATATCTG GGCCCTTGGT TGCGTTCTCT ATGAAATGTG CACACTTAAG 
CATGCATTTG AAGCTGGTAA CATGAAGAAC TTGGTACTGA AGATAATCTC TGGATCTTTC 
CCTCCTGTTT CTGTGCATTA TTCCTATGAC CTACGTAATC TACTGTCACA GTTATTTAAA 
AGGAATCCTA GGGATAGACC ATCAGTGAAC TCCATATTGG AGAAAAATTT CATAGCCAAA 
CGGGTTGAAA AATTTCTCAC ACCGCAGCTT ATTGCAGAAG AATTCAGTCA CAAAGTGTTT 
CACAAGTTTG GACCACATGC TGGACCAGCT AAAAGACCAG CTCAAGGACA TATCTCGGCT 
TCAATTGCAC CAGTGCAGAA AATTACCAAA CCTGCTGCAA AATACGGAGT GCCTTTAGCG 
ATGAAGAAGT GTTATGACGC ACCCAAAAAG CTTCATGAGA AAAAGTCATT GATTAAACTT 
AGGCAGGCCT TTCCAACTCC AAAGAAGAAA ATTATTCCAG GAGAAGAAAG GAGGAAAATG 
TTTGAGGAAC CTTCAAAAAA GAAAAGGTTA GAATTGCCTG AAAAAGAAAA ACGCCAGAGA 
GATCAGATCA GTTTACTGAA GGCAGAGGAA ATGAGAAGAT TGGAAAAAGA AAGGATGGAA 
CGAATAAACA GAGCCAGGGA GCAAGGATGG AGGAATGTGC TTGGTTCAGG TGGAAGTGGT 
GATGTTAAGG CACCATATTA TGGCGGTGGA GGTGGTGTTG GTCCTTTTCC TGCCTCTTTC 
AGAGGACAGT ATGAGCACTA TCATGCTATT TTTGACCAGA TGCAAAAGGA AACCTTCAAA 
GTAACTAAAG AGAGGCAAGC CAAATGGAGG GCGGAGGTAC AAGGCCAAGA AGCTGTTGAG 
AGGCAGAAAG GATGGCTACC TGCAGAAAGA GCAAAGCAAG TTGAAGAATT CTGGCAACGA 
AAGAGGGAAG CCATGCAAAA CAAAGCTCGT GCTGAGGGAC ACATGGAATA TTTGGCAAGA 
TTAAGACAGA TACGACTACA AAACTTCAAT GAGCGTCAAC AGATTAAAGC AAAACTTCGT 
GGAGAGAAGA ATGAAGCTCC CAGTTCTGAT GGACAAGAGT CAAGTGAGGA AGCAGAAATG 
AAACGCAAAA AGATAGAAGC ACAGAAGGCC CAAGCAAATG CTCGAGCTGC AGTTCTGAAA 
GAACAGTTAG AGCGAAAGAG GAAGGAGGCA TATGAAAGAG AGAAAAGAGC TTGGGAAGAA 
CATCTGATAG CAAAAGGAGT AAAGAATCCT AATGTGCCTT TTCATGTGGG AGCAGCAGGA 
CACACTCCTG TGCATGGAAT ACAAGAGCCT GGAGTGTCTC CTCCTAAGCA ACAGCTTCCA 
GTTAAGCCCA TCTCCATGAC TTCTGCTTTG AAAGAAGTTG GCGTGGATGG AAATGTAACC 
GCTGCCCAGG AGGTTAAAGA GGAAATAAAA AAGCCAGATC TTACAATGCA GAACAAGAGG 
CAGATACTGA AAAGATTGAA TCAAAATCTT AAAGCTCAGG AAGATGAGAA AGGAAGAAAG 
GACGATAAAA ATATCTCTGA GTCAGATGGG TCTGAAGATG GTAAAGAAGA GCAAGAAGAT 
AACCATCCAC TTCTGGGAGA TCGCAAAAGA TGGGCATCAA GGGCATCAGA TTTGGTAGTT 
CCTTTAGCTC AGTTATCAAT GGAAGATTCT CTCTCTGGAA CAGACCGGCA AACTATGGGG 
GAAGTAATCA GGCTAGATAT TGGGGAACCC CGCAGGAAAG TCTGGGGCAA AAGCCCTACT 
GATTCGGTCC TGAAGATTCT AGGAGAAGCG GAGTTGCAGC TACCAACTGA ATTACTGGAA 
GAAATAGATG TAATAAATGA GCCTGATGAT TTGGAAGCAG TGATGTTGAA AGAAACAGAA 
GAAGATAAAA AGCACCAGAG CAAGGAATTT CCCATTGCTG TTAATGAAGT GTGGGTGAAA 
GAAAAACAGG ATGAGGCACA ACAGGATGCA AGAAATGAAG CAGCTTTTGA GAAAGAAGTA 
GTGCTTGATG AAGTGCAATC ACAAAATGAA GCTCCAGAAG AAACTTGTTC CAGTGACTCT 
CTGCAACCTG AACCGTTATT CCAGAGAGTA ATACAGCCCC CAGCTGTACC TACAGCCTCA 
CCAGTTCTAT CATCTCAGTC TTCCCAAGAA GAGCCTTTTG TACCTCGTTC ACGTTCCATA 
TCGCCAGCAA AAAATAAAGG GAAAAGTTCA TTGTTTTTTG GACTTTCTAC AGGTCTGTTT 
GATGCAAACA ACCCAAAGAT GTTGAGAACG TGTTCACTCC CGGATCTTTA CAAACTGTAC 
AGAACCTTAG TTGATGTTCC CAGCATAGCA GATGTACAGC ATCAACACAG TCTTGAAATA 
GATGATACGG AAGATGAGCA AGCCAAAGAA GGACCCTCTG ATTCTGAGGA TATCATGTTT 
GGGGACGCCG ATACAGATTT GCAGGAACTC CGGGCCTCAA TGGAACAATT ACTTAGGGAG 
CAGCCTAGTG AGGAATTAAG TGAAGAGGAG GAATCTACTT TAAAGGCTAA CGTCATCAAA 
TGCATGACAA ATGGTACAGA GCTGGATGAA GCAGATGAAA ATAACCCCAG CAGTGAAAGT 
GCACTTAATG AAGAATGGCA GTCAGATAAT AGTGATGGAG ATATTGCCAG TGAATGTGAA 
GAATGTGATA GTGTCTTCAG CCATTTGGAA GAGCTGAGAT ACAACCTGGA ACAGGAAATA 
GGCTTTGAGA AATTAATTGA AGTTTATAAT AAAATAAAGG CCATACATGA AGATGAAGAT 
GAAAATATTG ATATTTGTTC AAGCATAGTT CAGACTATTC TGGGAAATGA ACACAAACAC 
TTATATGCCA AAGTACTTCA CCTGGTCATG GCGGATGGAG CCTACCAGGA AGATAATGAC 
GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_015285382.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015140868.1 Gallus gallus NIMA related kinase 1 (NEK1), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OGa07770
Clone ID Related Accession (Same CDS sequence)	XM_015285382.1 , XM_015285382.2
Accession Version	XM_015285382.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3486bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	serine/threonine-protein kinase Nek1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015285382.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAAAT ACATTAAAGT GCAAAAAATT GGAGAAGGAT CCTTTGGGAA AGCATTTCTT 
GTTAAAGATA AAGAAAATAG CCAGCAGTAT GTTATTAAAG AAATTAACAT ATCTAAGATG 
TCAAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TACTGGCCAA CATGAAACAT 
CCAAATATTG TCCTGTATAG GGAGTCGTTT GAAGAAAATG GTTGTCTCTA CATAGTGATG 
GATTACTGTG AAGGAGGAGA TCTGTTCAAA AAAATAAATG CCCAGAAAGG AGTCCTGTTC 
TCTGAGGATC AGATTCTTGA TTGGTTTGTA CAGATCTGTT TGGCACTGAA ACATATACAT 
GATAGAAAAA TCTTACATCG CGACATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA 
ACAATACAGC TGGGAGATTT TGGAATCGCG AGAGTTCTGA ACAGTACTGT GGAATTGGCT 
CGCACTTGCA TAGGAACACC ATACTATTTA TCACCTGAAA TATGCCAGAA CAGGCCTTAC 
AACAATAAAA GTGATATCTG GGCCCTTGGT TGCGTTCTCT ATGAAATGTG CACACTTAAG 
CATGCATTTG AAGCTGGTAA CATGAAGAAC TTGGTACTGA AGATAATCTC TGGATCTTTC 
CCTCCTGTTT CTGTGCATTA TTCCTATGAC CTACGTAATC TACTGTCACA GTTATTTAAA 
AGGAATCCTA GGGATAGACC ATCAGTGAAC TCCATATTGG AGAAAAATTT CATAGCCAAA 
CGGGTTGAAA AATTTCTCAC ACCGCAGCTT ATTGCAGAAG AATTCAGTCA CAAAGTGTTT 
CACAAGTTTG GACCACATGC TGGACCAGCT AAAAGACCAG CTCAAGGACA TATCTCGGCT 
TCAATTGCAC CAGTGCAGAA AATTACCAAA CCTGCTGCAA AATACGGAGT GCCTTTAGCG 
ATGAAGAAGT GTTATGACGC ACCCAAAAAG CTTCATGAGA AAAAGTCATT GATTAAACTT 
AGGCAGGCCT TTCCAACTCC AAAGAAGAAA ATTATTCCAG GAGAAGAAAG GAGGAAAATG 
TTTGAGGAAC CTTCAAAAAA GAAAAGGTTA GAATTGCCTG AAAAAGAAAA ACGCCAGAGA 
GATCAGATCA GTTTACTGAA GGCAGAGGAA ATGAGAAGAT TGGAAAAAGA AAGGATGGAA 
CGAATAAACA GAGCCAGGGA GCAAGGATGG AGGAATGTGC TTGGTTCAGG TGGAAGTGGT 
GATGTTAAGG CACCATATTA TGGCGGTGGA GGTGGTGTTG GTCCTTTTCC TGCCTCTTTC 
AGAGGACAGT ATGAGCACTA TCATGCTATT TTTGACCAGA TGCAAAAGGA AACCTTCAAA 
GTAACTAAAG AGAGGCAAGC CAAATGGAGG GCGGAGGTAC AAGGCCAAGA AGCTGTTGAG 
AGGCAGAAAG GATGGCTACC TGCAGAAAGA GCAAAGCAAG TTGAAGAATT CTGGCAACGA 
AAGAGGGAAG CCATGCAAAA CAAAGCTCGT GCTGAGGGAC ACATGGAATA TTTGGCAAGA 
TTAAGACAGA TACGACTACA AAACTTCAAT GAGCGTCAAC AGATTAAAGC AAAACTTCGT 
GGAGAGAAGA ATGAAGCTCC CAGTTCTGAT GGACAAGAGT CAAGTGAGGA AGCAGAAATG 
AAACGCAAAA AGATAGAAGC ACAGAAGGCC CAAGCAAATG CTCGAGCTGC AGTTCTGAAA 
GAACAGTTAG AGCGAAAGAG GAAGGAGGCA TATGAAAGAG AGAAAAGAGC TTGGGAAGAA 
CATCTGATAG CAAAAGGAGT AAAGAATCCT AATGTGCCTT TTCATGTGGG AGCAGCAGGA 
CACACTCCTG TGCATGGAAT ACAAGAGCCT GGAGTGTCTC CTCCTAAGCA ACAGCTTCCA 
GTTAAGCCCA TCTCCATGAC TTCTGCTTTG AAAGAAGTTG GCGTGGATGG AAATGTAACC 
GCTGCCCAGG AGGTTAAAGA GGAAATAAAA AAGCCAGATC TTACAATGCA GAACAAGAGG 
CAGATACTGA AAAGATTGAA TCAAAATCTT AAAGCTCAGG AAGATGAGAA AGGAAGAAAG 
GACGATAAAA ATATCTCTGA GTCAGATGGG TCTGAAGATG GTAAAGAAGA GCAAGAAGAT 
AACCATCCAC TTCTGGGAGA TCGCAAAAGA TGGGCATCAA GGGCATCAGA TTTGGTAGTT 
CCTTTAGCTC AGTTATCAAT GGAAGATTCT CTCTCTGGAA CAGACCGGCA AACTATGGGG 
GAAGTAATCA GGCTAGATAT TGGGGAACCC CGCAGGAAAG TCTGGGGCAA AAGCCCTACT 
GATTCGGTCC TGAAGATTCT AGGAGAAGCG GAGTTGCAGC TACCAACTGA ATTACTGGAA 
GAAATAGATG TAATAAATGA GCCTGATGAT TTGGAAGCAG TGATGTTGAA AGAAACAGAA 
GAAGATAAAA AGCACCAGAG CAAGGAATTT CCCATTGCTG TTAATGAAGT GTGGGTGAAA 
GAAAAACAGG ATGAGGCACA ACAGGATGCA AGAAATGAAG CAGCTTTTGA GAAAGAAGTA 
GTGCTTGATG AAGTGCAATC ACAAAATGAA GCTCCAGAAG AAACTTGTTC CAGTGACTCT 
CTGCAACCTG AACCGTTATT CCAGAGAGTA ATACAGCCCC CAGCTGTACC TACAGCCTCA 
CCAGTTCTAT CATCTCAGTC TTCCCAAGAA GAGCCTTTTG TACCTCGTTC ACGTTCCATA 
TCGCCAGCAA AAAATAAAGG GAAAAGTTCA TTGTTTTTTG GACTTTCTAC AGGTCTGTTT 
GATGCAAACA ACCCAAAGAT GTTGAGAACG TGTTCACTCC CGGATCTTTA CAAACTGTAC 
AGAACCTTAG TTGATGTTCC CAGCATAGCA GATGTACAGC ATCAACACAG TCTTGAAATA 
GATGATACGG AAGATGAGCA AGCCAAAGAA GGACCCTCTG ATTCTGAGGA TATCATGTTT 
GGGGACGCCG ATACAGATTT GCAGGAACTC CGGGCCTCAA TGGAACAATT ACTTAGGGAG 
CAGCCTAGTG AGGAATTAAG TGAAGAGGAG GAATCTACTT TAAAGGCTAA CGTCATCAAA 
TGCATGACAA ATGGTACAGA GCTGGATGAA GCAGATGAAA ATAACCCCAG CAGTGAAAGT 
GCACTTAATG AAGAATGGCA GTCAGATAAT AGTGATGGAG ATATTGCCAG TGAATGTGAA 
GAATGTGATA GTGTCTTCAG CCATTTGGAA GAGCTGAGAT ACAACCTGGA ACAGGAAATA 
GGCTTTGAGA AATTAATTGA AGTTTATAAT AAAATAAAGG CCATACATGA AGATGAAGAT 
GAAAATATTG ATATTTGTTC AAGCATAGTT CAGACTATTC TGGGAAATGA ACACAAACAC 
TTATATGCCA AAGTACTTCA CCTGGTCATG GCGGATGGAG CCTACCAGGA AGATAATGAC 
GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015140868.1
CDS	1003..4488
Translation	MDKYIKVQKIGEGSFGKAFLVKDKENSQQYVIKEINISKMSNKEREESRREVAVLANMKHPNIVLYRESFEENGCLYIVMDYCEGGDLFKKINAQKGVLFSEDQILDWFVQICLALKHIHDRKILHRDIKSQNIFLTKDGTIQLGDFGIARVLNSTVELARTCIGTPYYLSPEICQNRPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIISGSFPPVSVHYSYDLRNLLSQLFKRNPRDRPSVNSILEKNFIAKRVEKFLTPQLIAEEFSHKVFHKFGPHAGPAKRPAQGHISASIAPVQKITKPAAKYGVPLAMKKCYDAPKKLHEKKSLIKLRQAFPTPKKKIIPGEERRKMFEEPSKKKRLELPEKEKRQRDQISLLKAEEMRRLEKERMERINRAREQGWRNVLGSGGSGDVKAPYYGGGGGVGPFPASFRGQYEHYHAIFDQMQKETFKVTKERQAKWRAEVQGQEAVERQKGWLPAERAKQVEEFWQRKREAMQNKARAEGHMEYLARLRQIRLQNFNERQQIKAKLRGEKNEAPSSDGQESSEEAEMKRKKIEAQKAQANARAAVLKEQLERKRKEAYEREKRAWEEHLIAKGVKNPNVPFHVGAAGHTPVHGIQEPGVSPPKQQLPVKPISMTSALKEVGVDGNVTAAQEVKEEIKKPDLTMQNKRQILKRLNQNLKAQEDEKGRKDDKNISESDGSEDGKEEQEDNHPLLGDRKRWASRASDLVVPLAQLSMEDSLSGTDRQTMGEVIRLDIGEPRRKVWGKSPTDSVLKILGEAELQLPTELLEEIDVINEPDDLEAVMLKETEEDKKHQSKEFPIAVNEVWVKEKQDEAQQDARNEAAFEKEVVLDEVQSQNEAPEETCSSDSLQPEPLFQRVIQPPAVPTASPVLSSQSSQEEPFVPRSRSISPAKNKGKSSLFFGLSTGLFDANNPKMLRTCSLPDLYKLYRTLVDVPSIADVQHQHSLEIDDTEDEQAKEGPSDSEDIMFGDADTDLQELRASMEQLLREQPSEELSEEEESTLKANVIKCMTNGTELDEADENNPSSESALNEEWQSDNSDGDIASECEECDSVFSHLEELRYNLEQEIGFEKLIEVYNKIKAIHEDEDENIDICSSIVQTILGNEHKHLYAKVLHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_015285382.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus NIMA related kinase 1 (NEK1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015285382.2

ORF Insert Sequence:

ATGGATAAAT ACATTAAAGT GCAAAAAATT GGAGAAGGAT CCTTTGGGAA AGCATTTCTT 
GTTAAAGATA AAGAAAATAG CCAGCAGTAT GTTATTAAAG AAATTAACAT ATCTAAGATG 
TCAAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TACTGGCCAA CATGAAACAT 
CCAAATATTG TCCTGTATAG GGAGTCGTTT GAAGAAAATG GTTGTCTCTA CATAGTGATG 
GATTACTGTG AAGGAGGAGA TCTGTTCAAA AAAATAAATG CCCAGAAAGG AGTCCTGTTC 
TCTGAGGATC AGATTCTTGA TTGGTTTGTA CAGATCTGTT TGGCACTGAA ACATATACAT 
GATAGAAAAA TCTTACATCG CGACATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA 
ACAATACAGC TGGGAGATTT TGGAATCGCG AGAGTTCTGA ACAGTACTGT GGAATTGGCT 
CGCACTTGCA TAGGAACACC ATACTATTTA TCACCTGAAA TATGCCAGAA CAGGCCTTAC 
AACAATAAAA GTGATATCTG GGCCCTTGGT TGCGTTCTCT ATGAAATGTG CACACTTAAG 
CATGCATTTG AAGCTGGTAA CATGAAGAAC TTGGTACTGA AGATAATCTC TGGATCTTTC 
CCTCCTGTTT CTGTGCATTA TTCCTATGAC CTACGTAATC TACTGTCACA GTTATTTAAA 
AGGAATCCTA GGGATAGACC ATCAGTGAAC TCCATATTGG AGAAAAATTT CATAGCCAAA 
CGGGTTGAAA AATTTCTCAC ACCGCAGCTT ATTGCAGAAG AATTCAGTCA CAAAGTGTTT 
CACAAGTTTG GACCACATGC TGGACCAGCT AAAAGACCAG CTCAAGGACA TATCTCGGCT 
TCAATTGCAC CAGTGCAGAA AATTACCAAA CCTGCTGCAA AATACGGAGT GCCTTTAGCG 
ATGAAGAAGT GTTATGACGC ACCCAAAAAG CTTCATGAGA AAAAGTCATT GATTAAACTT 
AGGCAGGCCT TTCCAACTCC AAAGAAGAAA ATTATTCCAG GAGAAGAAAG GAGGAAAATG 
TTTGAGGAAC CTTCAAAAAA GAAAAGGTTA GAATTGCCTG AAAAAGAAAA ACGCCAGAGA 
GATCAGATCA GTTTACTGAA GGCAGAGGAA ATGAGAAGAT TGGAAAAAGA AAGGATGGAA 
CGAATAAACA GAGCCAGGGA GCAAGGATGG AGGAATGTGC TTGGTTCAGG TGGAAGTGGT 
GATGTTAAGG CACCATATTA TGGCGGTGGA GGTGGTGTTG GTCCTTTTCC TGCCTCTTTC 
AGAGGACAGT ATGAGCACTA TCATGCTATT TTTGACCAGA TGCAAAAGGA AACCTTCAAA 
GTAACTAAAG AGAGGCAAGC CAAATGGAGG GCGGAGGTAC AAGGCCAAGA AGCTGTTGAG 
AGGCAGAAAG GATGGCTACC TGCAGAAAGA GCAAAGCAAG TTGAAGAATT CTGGCAACGA 
AAGAGGGAAG CCATGCAAAA CAAAGCTCGT GCTGAGGGAC ACATGGAATA TTTGGCAAGA 
TTAAGACAGA TACGACTACA AAACTTCAAT GAGCGTCAAC AGATTAAAGC AAAACTTCGT 
GGAGAGAAGA ATGAAGCTCC CAGTTCTGAT GGACAAGAGT CAAGTGAGGA AGCAGAAATG 
AAACGCAAAA AGATAGAAGC ACAGAAGGCC CAAGCAAATG CTCGAGCTGC AGTTCTGAAA 
GAACAGTTAG AGCGAAAGAG GAAGGAGGCA TATGAAAGAG AGAAAAGAGC TTGGGAAGAA 
CATCTGATAG CAAAAGGAGT AAAGAATCCT AATGTGCCTT TTCATGTGGG AGCAGCAGGA 
CACACTCCTG TGCATGGAAT ACAAGAGCCT GGAGTGTCTC CTCCTAAGCA ACAGCTTCCA 
GTTAAGCCCA TCTCCATGAC TTCTGCTTTG AAAGAAGTTG GCGTGGATGG AAATGTAACC 
GCTGCCCAGG AGGTTAAAGA GGAAATAAAA AAGCCAGATC TTACAATGCA GAACAAGAGG 
CAGATACTGA AAAGATTGAA TCAAAATCTT AAAGCTCAGG AAGATGAGAA AGGAAGAAAG 
GACGATAAAA ATATCTCTGA GTCAGATGGG TCTGAAGATG GTAAAGAAGA GCAAGAAGAT 
AACCATCCAC TTCTGGGAGA TCGCAAAAGA TGGGCATCAA GGGCATCAGA TTTGGTAGTT 
CCTTTAGCTC AGTTATCAAT GGAAGATTCT CTCTCTGGAA CAGACCGGCA AACTATGGGG 
GAAGTAATCA GGCTAGATAT TGGGGAACCC CGCAGGAAAG TCTGGGGCAA AAGCCCTACT 
GATTCGGTCC TGAAGATTCT AGGAGAAGCG GAGTTGCAGC TACCAACTGA ATTACTGGAA 
GAAATAGATG TAATAAATGA GCCTGATGAT TTGGAAGCAG TGATGTTGAA AGAAACAGAA 
GAAGATAAAA AGCACCAGAG CAAGGAATTT CCCATTGCTG TTAATGAAGT GTGGGTGAAA 
GAAAAACAGG ATGAGGCACA ACAGGATGCA AGAAATGAAG CAGCTTTTGA GAAAGAAGTA 
GTGCTTGATG AAGTGCAATC ACAAAATGAA GCTCCAGAAG AAACTTGTTC CAGTGACTCT 
CTGCAACCTG AACCGTTATT CCAGAGAGTA ATACAGCCCC CAGCTGTACC TACAGCCTCA 
CCAGTTCTAT CATCTCAGTC TTCCCAAGAA GAGCCTTTTG TACCTCGTTC ACGTTCCATA 
TCGCCAGCAA AAAATAAAGG GAAAAGTTCA TTGTTTTTTG GACTTTCTAC AGGTCTGTTT 
GATGCAAACA ACCCAAAGAT GTTGAGAACG TGTTCACTCC CGGATCTTTA CAAACTGTAC 
AGAACCTTAG TTGATGTTCC CAGCATAGCA GATGTACAGC ATCAACACAG TCTTGAAATA 
GATGATACGG AAGATGAGCA AGCCAAAGAA GGACCCTCTG ATTCTGAGGA TATCATGTTT 
GGGGACGCCG ATACAGATTT GCAGGAACTC CGGGCCTCAA TGGAACAATT ACTTAGGGAG 
CAGCCTAGTG AGGAATTAAG TGAAGAGGAG GAATCTACTT TAAAGGCTAA CGTCATCAAA 
TGCATGACAA ATGGTACAGA GCTGGATGAA GCAGATGAAA ATAACCCCAG CAGTGAAAGT 
GCACTTAATG AAGAATGGCA GTCAGATAAT AGTGATGGAG ATATTGCCAG TGAATGTGAA 
GAATGTGATA GTGTCTTCAG CCATTTGGAA GAGCTGAGAT ACAACCTGGA ACAGGAAATA 
GGCTTTGAGA AATTAATTGA AGTTTATAAT AAAATAAAGG CCATACATGA AGATGAAGAT 
GAAAATATTG ATATTTGTTC AAGCATAGTT CAGACTATTC TGGGAAATGA ACACAAACAC 
TTATATGCCA AAGTACTTCA CCTGGTCATG GCGGATGGAG CCTACCAGGA AGATAATGAC 
GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	NEK1
Entrez Gene ID	422433
Full Name	NIMA related kinase 1
General protein information	Preferred Names NIMA related kinase 1 Names serine/threonine-protein kinase Nek1 NIMA (never in mitosis gene a)-related kinase 1
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

Bos taurus (cattle)	NEK1	XP_003586402.2
Rattus norvegicus (Norway rat)	Nek1	NP_001099552.2
Gallus gallus (chicken)	NEK1	XP_420401.3
Homo sapiens (human)	NEK1	NP_001186326.1
	NEK1	XP_001083275.1
Canis lupus familiaris (dog)	NEK1	XP_543184.2
Mus musculus (house mouse)	Nek1	NP_780298.2
Pan troglodytes (chimpanzee)	NEK1	XP_526727.2
Danio rerio (zebrafish)	nek1	XP_005160601.1
Xenopus tropicalis (tropical clawed frog)	nek1	NP_001090680.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

NEK1 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

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