asah2 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	asah2
Entrez Gene ID	493602
Full Name	N-acylsphingosine amidohydrolase 2
General protein information	Preferred Names N-acylsphingosine amidohydrolase 2 Names neutral ceramidase N-CDase NCDase acylsphingosine deacylase 2 non-lysosomal ceramidase znCD
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001007763.1	NP_001007764.1	neutral ceramidase

Pathways from BioSystems

Homologies

Homo sapiens (human)	ASAH2	NP_063946.2
Drosophila melanogaster (fruit fly)	CDase	NP_733370.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000973	XP_309165.3
Arabidopsis thaliana (thale cress)	AT2G38010	NP_181337.2
Xenopus tropicalis (tropical clawed frog)	asah2	NP_001123830.1
Xenopus tropicalis (tropical clawed frog)	LOC100494440	XP_002935475.2
	ASAH2	XP_001100613.1
Bos taurus (cattle)	ASAH2	XP_002698412.1
Mus musculus (house mouse)	Asah2	NP_061300.1
Rattus norvegicus (Norway rat)	Asah2	NP_446098.1
Danio rerio (zebrafish)	asah2	NP_001007764.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003730	XP_310261.7
Gallus gallus (chicken)	ASAH2	XP_421560.4
Oryza sativa (rice)	Os01g0624000	NP_001043618.1
Pan troglodytes (chimpanzee)	ASAH2	XP_507791.2
Canis lupus familiaris (dog)	ASAH2B	XP_005636694.1

Gene Ontology
Bibliography

Related articles in PubMed

The ceramide synthase 2b gene mediates genomic sensing and regulation of sphingosine levels during zebrafish embryogenesis.
Mendelson K, Pandey S, Hisano Y, Carellini F, Das BC, Hla T, Evans T
eLife6(2017 Sep)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Molecular cloning and functional analysis of zebrafish neutral ceramidase.
Yoshimura Y, Tani M, Okino N, Iida H, Ito M
The Journal of biological chemistry279(42)44012-22(2004 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

essential for metabolism of ceramide and the early development of zebrafish.
Title: Molecular cloning and functional analysis of zebrafish neutral ceramidase.

The following asah2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the asah2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa37459	NM_001007763.1 Latest version!	Danio rerio N-acylsphingosine amidohydrolase 2 (asah2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

asah2 ( NM_001007763.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001007764.1 Danio rerio N-acylsphingosine amidohydrolase 2 (asah2), mRNA.

Price & Availability↑

CloneID	ODa37459
Clone ID Related Accession (Same CDS sequence)	NM_001007763.1
Accession Version	NM_001007763.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2232bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-02
Organism	Danio rerio(zebrafish)
Product	neutral ceramidase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB194413.1. ##Evidence-Data-START## Transcript exon combination :: AB194413.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA3505370, SAMEA3505371 [ECO:0000348] ##Evidence-Data-END##

ATGGCGAGTA AGAGTCGTCG TCTCAGCGGA CTGGAGATCT CACTCATTGT TCTCTTTCTG 
TTGATGACGG CAGTTTCAGT GGCACTGATC ACCGTCCTGG CCCTCAAACA GGAATCAGAT 
AAAAAAGAAG AAGTGACTCC GGAAGAACCG AGCCCATCAG TAACTCCTCC AGAAAAGCCT 
TATCTGATTG GTGTGGGTCG AGCGGACTGT ACTGGACCAG TGGCAGATTT ACCTATGATG 
GGTTATGCCA ACACCGATCA GACGGCAAGA GGACTGCACA CGCGGTTGTT CAGCAGAGCC 
TTCATTGTGG ATGATGGCAA TAAGAGAGTC GTGTTCGTCA CCTCAGACAT CGGCATGGTC 
TCGCAGAGGC TCAGGCTAGA GGTTTTCCAA GCACTTAAGG AGAAATACGG AGACCTCTAC 
AGACAGGACA ATGTGGTGTT GAGTGGCACA CACACACATT CAGGAGTGGG TGGATATTTC 
CAGTACACAC TCTTTATGAT CACCAGCAAA GGCTACATCA AGCCTTCTAT ACAGGCCATC 
GTCAGTGGAA TAGTCAAGAG TATTGATATT GCTCATAGGA ATTTGAGGCC TGGACGAATA 
TTCATAAATA AAGGACAAGT AGCAGACAGC AACTTCAACA GAAGCCCACA TTCATACATG 
AACAATCCGG AAGAGGAGAG AAACAGATAT GAATTCAACA CAGACAAGCA GATTGTCGTC 
CTGAAATTCA CAGACCTGGA TGGGGATGGA ATAGGATTGC TCAGCTGGTT TGCTGTTCAT 
CCAGTCAGTA TGAATTACAC CAATCGCATG GTGAGCTCGG ACAACCTCGG CTACGCTTCC 
TACATCTTCG AGCAGGAGAA AAATATTGGC TTTCTGCCCG GCGAAAAGGG TCCTTTTGTG 
GCTGGATTTT CCTCCAGTAA TCTTGGAGAT TCATCTCCTA ATATTCGCGG CCCGGTCTGT 
GTGAACACAG GATTGAAGTG TGACTACATT AACAGCTCTT GCCCTGTTGG AGGAAAAAAA 
GCATGCATAG CATTTGGACC GGGTGAAGAC ATGTTCGAAA GCACCAGGAT CATAGGAGAG 
AATATGTTTA AAATAGCAAA GGAGCTGTAT GGCAGTGCAA AGCAGGAGTT ACACGGGCCT 
GTTTATGGTG CACATCAGTG GGTCAACATG ACTGATGAGA CAGTCCAGTT CAACTCCACT 
CATACAGGGC GGACGTGTAA ACCAGCTCTG GGTCACAGTT TTGCTGCTGG TACAACTGAT 
GGAGGAGGAG AATTCAACTT TTTACAAGGG GATACGGAAG GAGACCCTTT CTGGGATGGC 
ATAAGAGATG CTGTTTTGGG ACCACCATCC AATGAAACCA AGGCTTGCCA TCAGCCTAAA 
CCAATCCTCT TCAGCACTGG GGAGATGGAT TCACCTCTTC CATGGCATCC AGCCATTGTA 
GACGTCCAGA TCATCACTAT CGGCTCTTTA GCGGTAGTCG CCGTCCCGGG AGAATTTACT 
ACCATGTCAG GAAGGAGGAT AAGAGAAGCC GTCAAAAGGG AGCTGGAGGT AAAGGAACCT 
TTCACTAACG CAGAAGTGGT GGTCGCTGGT CTGTGCAACA TCTACACCCA CTACATCACC 
ACATATGAAG AGTACCAGAT CCAGCGATAT GAAGGAGCAT CCACCATTTT TGGCCCTCAC 
ACTCTCTCCG CGTACATCCA GCGCTACAGA GGCCTGGCCA AAGCCATAGC TCATGGGACA 
ATAGGAGAGC TGCCAAAGGG ACCCGAACCC CCTTTCTTTG ATGAAGACAA ACTTTTTAAT 
CAAGTAAGAG ACCCGGTGGC AGATGTAGCA CCAGTTGGCA CCACCTTTGG AGATGTCTTG 
CAGGAGGTTA ACCCTGTCTA CAAAGTGGGT GAAATCGCAT CCGTCACATT TGTTTCTGGA 
AACCCGAGGC ACTCTGGAGA TATTAGAGAT ACGACACTGG TCACCGTGGA GAGGTTTCAC 
AACGACACAG GCTCATGGGA AATAATTCAC AACGACGCAT CATGGGAAAC AAGGTTTCAC 
TGGATTAAAG GACTCGCGGG CAGGAGTCAG GCTAAAGTGG AGTGGCACAT CCCGCAAACA 
GCTCAAGCAG GAACCTACCA GATTCAGTAC TTTGGACATT ATAAACAAAC CACAGAAAAC 
ACCACAGTCA TCACACCATA CGTGGGCACT TCTGCTGCTT TTAAAGTCGC CAGAAGTTTC 
TACTACTTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001007764.1
CDS	113..2344
Translation	MASKSRRLSGLEISLIVLFLLMTAVSVALITVLALKQESDKKEEVTPEEPSPSVTPPEKPYLIGVGRADCTGPVADLPMMGYANTDQTARGLHTRLFSRAFIVDDGNKRVVFVTSDIGMVSQRLRLEVFQALKEKYGDLYRQDNVVLSGTHTHSGVGGYFQYTLFMITSKGYIKPSIQAIVSGIVKSIDIAHRNLRPGRIFINKGQVADSNFNRSPHSYMNNPEEERNRYEFNTDKQIVVLKFTDLDGDGIGLLSWFAVHPVSMNYTNRMVSSDNLGYASYIFEQEKNIGFLPGEKGPFVAGFSSSNLGDSSPNIRGPVCVNTGLKCDYINSSCPVGGKKACIAFGPGEDMFESTRIIGENMFKIAKELYGSAKQELHGPVYGAHQWVNMTDETVQFNSTHTGRTCKPALGHSFAAGTTDGGGEFNFLQGDTEGDPFWDGIRDAVLGPPSNETKACHQPKPILFSTGEMDSPLPWHPAIVDVQIITIGSLAVVAVPGEFTTMSGRRIREAVKRELEVKEPFTNAEVVVAGLCNIYTHYITTYEEYQIQRYEGASTIFGPHTLSAYIQRYRGLAKAIAHGTIGELPKGPEPPFFDEDKLFNQVRDPVADVAPVGTTFGDVLQEVNPVYKVGEIASVTFVSGNPRHSGDIRDTTLVTVERFHNDTGSWEIIHNDASWETRFHWIKGLAGRSQAKVEWHIPQTAQAGTYQIQYFGHYKQTTENTTVITPYVGTSAAFKVARSFYYF

Target ORF information:

RefSeq Version	NM_001007763.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio N-acylsphingosine amidohydrolase 2 (asah2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001007763.1

ORF Insert Sequence:

ATGGCGAGTA AGAGTCGTCG TCTCAGCGGA CTGGAGATCT CACTCATTGT TCTCTTTCTG 
TTGATGACGG CAGTTTCAGT GGCACTGATC ACCGTCCTGG CCCTCAAACA GGAATCAGAT 
AAAAAAGAAG AAGTGACTCC GGAAGAACCG AGCCCATCAG TAACTCCTCC AGAAAAGCCT 
TATCTGATTG GTGTGGGTCG AGCGGACTGT ACTGGACCAG TGGCAGATTT ACCTATGATG 
GGTTATGCCA ACACCGATCA GACGGCAAGA GGACTGCACA CGCGGTTGTT CAGCAGAGCC 
TTCATTGTGG ATGATGGCAA TAAGAGAGTC GTGTTCGTCA CCTCAGACAT CGGCATGGTC 
TCGCAGAGGC TCAGGCTAGA GGTTTTCCAA GCACTTAAGG AGAAATACGG AGACCTCTAC 
AGACAGGACA ATGTGGTGTT GAGTGGCACA CACACACATT CAGGAGTGGG TGGATATTTC 
CAGTACACAC TCTTTATGAT CACCAGCAAA GGCTACATCA AGCCTTCTAT ACAGGCCATC 
GTCAGTGGAA TAGTCAAGAG TATTGATATT GCTCATAGGA ATTTGAGGCC TGGACGAATA 
TTCATAAATA AAGGACAAGT AGCAGACAGC AACTTCAACA GAAGCCCACA TTCATACATG 
AACAATCCGG AAGAGGAGAG AAACAGATAT GAATTCAACA CAGACAAGCA GATTGTCGTC 
CTGAAATTCA CAGACCTGGA TGGGGATGGA ATAGGATTGC TCAGCTGGTT TGCTGTTCAT 
CCAGTCAGTA TGAATTACAC CAATCGCATG GTGAGCTCGG ACAACCTCGG CTACGCTTCC 
TACATCTTCG AGCAGGAGAA AAATATTGGC TTTCTGCCCG GCGAAAAGGG TCCTTTTGTG 
GCTGGATTTT CCTCCAGTAA TCTTGGAGAT TCATCTCCTA ATATTCGCGG CCCGGTCTGT 
GTGAACACAG GATTGAAGTG TGACTACATT AACAGCTCTT GCCCTGTTGG AGGAAAAAAA 
GCATGCATAG CATTTGGACC GGGTGAAGAC ATGTTCGAAA GCACCAGGAT CATAGGAGAG 
AATATGTTTA AAATAGCAAA GGAGCTGTAT GGCAGTGCAA AGCAGGAGTT ACACGGGCCT 
GTTTATGGTG CACATCAGTG GGTCAACATG ACTGATGAGA CAGTCCAGTT CAACTCCACT 
CATACAGGGC GGACGTGTAA ACCAGCTCTG GGTCACAGTT TTGCTGCTGG TACAACTGAT 
GGAGGAGGAG AATTCAACTT TTTACAAGGG GATACGGAAG GAGACCCTTT CTGGGATGGC 
ATAAGAGATG CTGTTTTGGG ACCACCATCC AATGAAACCA AGGCTTGCCA TCAGCCTAAA 
CCAATCCTCT TCAGCACTGG GGAGATGGAT TCACCTCTTC CATGGCATCC AGCCATTGTA 
GACGTCCAGA TCATCACTAT CGGCTCTTTA GCGGTAGTCG CCGTCCCGGG AGAATTTACT 
ACCATGTCAG GAAGGAGGAT AAGAGAAGCC GTCAAAAGGG AGCTGGAGGT AAAGGAACCT 
TTCACTAACG CAGAAGTGGT GGTCGCTGGT CTGTGCAACA TCTACACCCA CTACATCACC 
ACATATGAAG AGTACCAGAT CCAGCGATAT GAAGGAGCAT CCACCATTTT TGGCCCTCAC 
ACTCTCTCCG CGTACATCCA GCGCTACAGA GGCCTGGCCA AAGCCATAGC TCATGGGACA 
ATAGGAGAGC TGCCAAAGGG ACCCGAACCC CCTTTCTTTG ATGAAGACAA ACTTTTTAAT 
CAAGTAAGAG ACCCGGTGGC AGATGTAGCA CCAGTTGGCA CCACCTTTGG AGATGTCTTG 
CAGGAGGTTA ACCCTGTCTA CAAAGTGGGT GAAATCGCAT CCGTCACATT TGTTTCTGGA 
AACCCGAGGC ACTCTGGAGA TATTAGAGAT ACGACACTGG TCACCGTGGA GAGGTTTCAC 
AACGACACAG GCTCATGGGA AATAATTCAC AACGACGCAT CATGGGAAAC AAGGTTTCAC 
TGGATTAAAG GACTCGCGGG CAGGAGTCAG GCTAAAGTGG AGTGGCACAT CCCGCAAACA 
GCTCAAGCAG GAACCTACCA GATTCAGTAC TTTGGACATT ATAAACAAAC CACAGAAAAC 
ACCACAGTCA TCACACCATA CGTGGGCACT TCTGCTGCTT TTAAAGTCGC CAGAAGTTTC 
TACTACTTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The ceramide synthase 2b gene mediates genomic sensing and regulation of sphingosine levels during zebrafish embryogenesis.
eLife6(2017 Sep)
Mendelson K,Pandey S,Hisano Y,Carellini F,Das BC,Hla T,Evans T

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Molecular cloning and functional analysis of zebrafish neutral ceramidase.
The Journal of biological chemistry279(42)44012-22(2004 Oct)
Yoshimura Y,Tani M,Okino N,Iida H,Ito M

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asah2 cDNA ORF clone, Danio rerio(zebrafish)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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