ASAH2 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	ASAH2
Entrez Gene ID	100336736
Full Name	N-acylsphingosine amidohydrolase 2
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015469495.1	XP_015324981.1	neutral ceramidase
XM_015460595.2	XP_015316081.1	putative neutral ceramidase C
XM_015460595.1	XP_015316081.1	neutral ceramidase

Pathways from BioSystems

Homologies

Homo sapiens (human)	ASAH2	NP_063946.2
Drosophila melanogaster (fruit fly)	CDase	NP_733370.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000973	XP_309165.3
Arabidopsis thaliana (thale cress)	AT2G38010	NP_181337.2
Xenopus tropicalis (tropical clawed frog)	asah2	NP_001123830.1
Xenopus tropicalis (tropical clawed frog)	LOC100494440	XP_002935475.2
	ASAH2	XP_001100613.1
Bos taurus (cattle)	ASAH2	XP_002698412.1
Mus musculus (house mouse)	Asah2	NP_061300.1
Rattus norvegicus (Norway rat)	Asah2	NP_446098.1
Danio rerio (zebrafish)	asah2	NP_001007764.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003730	XP_310261.7
Gallus gallus (chicken)	ASAH2	XP_421560.4
Oryza sativa (rice)	Os01g0624000	NP_001043618.1
Pan troglodytes (chimpanzee)	ASAH2	XP_507791.2
Canis lupus familiaris (dog)	ASAH2B	XP_005636694.1

Gene Ontology

The following ASAH2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ASAH2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa231132	XM_015460595.2 Latest version!	Bos taurus N-acylsphingosine amidohydrolase 2 (ASAH2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa88642	XM_015469495.1 Latest version!	Bos taurus N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2 (ASAH2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OBa88642	XM_015460595.1 Latest version!	Bos taurus N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2 (ASAH2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ASAH2 ( XM_015460595.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015316081.1 Bos taurus N-acylsphingosine amidohydrolase 2 (ASAH2), mRNA.

Price & Availability↑

CloneID	OBa231132
Clone ID Related Accession (Same CDS sequence)	XM_015460595.2
Accession Version	XM_015460595.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2340bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	putative neutral ceramidase C
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037353.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015460595.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAC GCACCTTTTC CAGCTTTGAA ATATTCCTGA TTTTCCTCCT TGTGATAACG 
ACAGGGATCA CGGTGGCTCT TCTCAGCCTG TTGTTTATCA CCAGTGGGAC CATTGAAAAT 
CACACAGGCA AGTATCGTTT TCCATCCACC CAGGGCCCTC CAGCTACCCA GAGCCCTCCA 
GCTACCCAGG GCTCTGCAGC CCCTCAAAAC TCAACACCCT CCCGGGGCCC CACAACTACC 
CAGGCATCTC CAACACCCAG CACCCCAGAG CCTACCCTGT TTCAGAACTT CAGTGGCTAC 
CATATTGGTG TTGGGCGAGC TGACTGCACA GGACAAGTAT CAGATATTAA TTTGATGGGC 
TACGCCAAAA CTGGCCAGTA TGCACGGGGC ATCCTCACGA GGTTGTACAG TCGTGCGTTC 
ATCATGGCGG AGCCGGATGG GTCCAATCGC ATAGTGTTTG TCAGCATCGA CATAGGCATG 
GTATCCCAGC GACTCAGGCT GGAGGTCCTG AGCAGGCTGC AGAATAAATA CGGCTCCCTG 
TACAGAAGAG ACAATGTTAT TCTGAGTGGC ACTCATACAC ACTCGGGTCC AGCAGGATAT 
TTCCAGTACA CGACATTTGT AATTGCCAGT GAAGGATTTA GCAATCGAAC TTTTGAGTAC 
ATGGTCACTG GCATTGTGGA GAGCGTTGAG ATAGCACACA AAAATATGAA ACCAGGCAAA 
ATCTTTATCA ATAAAGGGAC TGTGGATGGT GCACAGATCA ACCGAAGTCC TACTTCTTAC 
CTTTGGAATC CACAGTCAGA GAGAGCAAGG TATTCTTCAA ATACAGACAA GGAAATGGTG 
CTCCTGAAAA TGGTAGATCT GAATGGAGAA GAGCTGGGTC TTATCAGCTG GTTTGCCATC 
CACCCAGTCA GCATGAAAAA CACCAATCAT CTTGTAAATA GTGACAACGT AGGTTATGCA 
TCTTACCTTT TTGAGCAAGA GAAGAACAAA GGTTATCTAC CTGGAGAGGG ACCGTATGTA 
GCAGCTTTTG CTTCATCAAA TCTGGGAGAT GTTTCCCCCA ATGTTCTTGG CCCGCAATGC 
ACCAACACGG GAGAGTCTTG TGATAACGCC AATAGCTCTT GTCCCATTGG TGGGCCTAGC 
ATGTGTGTGG CTATGGGACC TGGACAGGAT ATGTTTGAAA GTACACAAAT CATAGGACGG 
ATAATATATG ACAAAGCAAA GGAACTGTAT GAGTCTGCCT CCCAGGAGGT AACTGGGCCA 
CTGGCTTCAG CTCATCAGTG GGTGAATATG ACGGATGTCA CCGTCTGGCT CAATTCAACA 
CACACAGTAA AAACGTGTAA ACCAGCCTTG GGCTACAGTT TTGCAGCTGG TACAATCGAT 
GGATTTGGAA CCGTCAGTTT TACTCAGGGG ATGACAGAAG GCGATTCATT TTGGGACACT 
CTTCGGGATC AGGTTCTGGG CAAGCCATCT GAAGAAATCA AAGAATGCCA TAAACCAAAG 
CCAGTCCTTC TTCAGACTGG AGAGCTGTTA AATCCTCATC CCTGGCACCC AGATATCGTT 
GACGTTCAGA TTGTTACTCT CGGGTTCTTG GCCATCACTG CCATCCCTGG GGAGTTCACG 
ACCATGTCTG GACGGAGACT TCGGCAGGCA GTTCGAGAAG AATTTGAAGC TTATGGGATG 
CTGAATATGA CTGTTGTTAT TTCAGGTCTA TGTAATGTAT ATACACACTA CATTACCACA 
TATGAAGAAT ACCAGGCGCA GCGGTACGAG GCCGCATCCA CAATTTATGG ACCGCATACT 
CTGTCTGCCT ACATCCAGCT TTTCAGAGTC CTTGCCAAGG CCATTGCTAC GGATACTGTA 
GCCAATTTGA GCAGTGGTCC AAAGCCTCCA TTTTTTGAAG AACTGATGTC TCCATTAATT 
CCAAATATTG TGGATAGAGC ACCTGAAGGC ACAACTTTTG GGGATGTCCT GCTACCAGCG 
AACACTATAT ACAGAGTGGG AGAAGTTGTT GAAGTTACCT TTGTAGGTGC TAACCCAAAG 
AATTCAGCAG AAAACCGGAC CCATCAGACC TTCCTCACTG TGGAGAAATA TGAGGCCACA 
TCAGCAACAT GGCAGATAAT GCATAATGAT GCCTCCTGGG AGACTCGTTT CTACTGGCAC 
AAAGGCTCAC TGGGTCTTAG CAATGCGACG ATACAGTGGC ACATTCCAGA TACTGCCCAG 
CCTGGAACCT ACAGAATAAA ATACTTTGGA CACAGTCGGA AGCAGGACTT ACTCAAGCCC 
GCTGTTCTAC TTTCATTTGA AAGCACTCCC TCTGTTTTTG AAGTCACAAC TACTTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015316081.1
CDS	169..2508
Translation	MAKRTFSSFEIFLIFLLVITTGITVALLSLLFITSGTIENHTGKYRFPSTQGPPATQSPPATQGSAAPQNSTPSRGPTTTQASPTPSTPEPTLFQNFSGYHIGVGRADCTGQVSDINLMGYAKTGQYARGILTRLYSRAFIMAEPDGSNRIVFVSIDIGMVSQRLRLEVLSRLQNKYGSLYRRDNVILSGTHTHSGPAGYFQYTTFVIASEGFSNRTFEYMVTGIVESVEIAHKNMKPGKIFINKGTVDGAQINRSPTSYLWNPQSERARYSSNTDKEMVLLKMVDLNGEELGLISWFAIHPVSMKNTNHLVNSDNVGYASYLFEQEKNKGYLPGEGPYVAAFASSNLGDVSPNVLGPQCTNTGESCDNANSSCPIGGPSMCVAMGPGQDMFESTQIIGRIIYDKAKELYESASQEVTGPLASAHQWVNMTDVTVWLNSTHTVKTCKPALGYSFAAGTIDGFGTVSFTQGMTEGDSFWDTLRDQVLGKPSEEIKECHKPKPVLLQTGELLNPHPWHPDIVDVQIVTLGFLAITAIPGEFTTMSGRRLRQAVREEFEAYGMLNMTVVISGLCNVYTHYITTYEEYQAQRYEAASTIYGPHTLSAYIQLFRVLAKAIATDTVANLSSGPKPPFFEELMSPLIPNIVDRAPEGTTFGDVLLPANTIYRVGEVVEVTFVGANPKNSAENRTHQTFLTVEKYEATSATWQIMHNDASWETRFYWHKGSLGLSNATIQWHIPDTAQPGTYRIKYFGHSRKQDLLKPAVLLSFESTPSVFEVTTTW

Target ORF information:

RefSeq Version	XM_015460595.2
Organism	Bos taurus(cattle)
Definition	Bos taurus N-acylsphingosine amidohydrolase 2 (ASAH2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015460595.2

ORF Insert Sequence:

ATGGCCAAAC GCACCTTTTC CAGCTTTGAA ATATTCCTGA TTTTCCTCCT TGTGATAACG 
ACAGGGATCA CGGTGGCTCT TCTCAGCCTG TTGTTTATCA CCAGTGGGAC CATTGAAAAT 
CACACAGGCA AGTATCGTTT TCCATCCACC CAGGGCCCTC CAGCTACCCA GAGCCCTCCA 
GCTACCCAGG GCTCTGCAGC CCCTCAAAAC TCAACACCCT CCCGGGGCCC CACAACTACC 
CAGGCATCTC CAACACCCAG CACCCCAGAG CCTACCCTGT TTCAGAACTT CAGTGGCTAC 
CATATTGGTG TTGGGCGAGC TGACTGCACA GGACAAGTAT CAGATATTAA TTTGATGGGC 
TACGCCAAAA CTGGCCAGTA TGCACGGGGC ATCCTCACGA GGTTGTACAG TCGTGCGTTC 
ATCATGGCGG AGCCGGATGG GTCCAATCGC ATAGTGTTTG TCAGCATCGA CATAGGCATG 
GTATCCCAGC GACTCAGGCT GGAGGTCCTG AGCAGGCTGC AGAATAAATA CGGCTCCCTG 
TACAGAAGAG ACAATGTTAT TCTGAGTGGC ACTCATACAC ACTCGGGTCC AGCAGGATAT 
TTCCAGTACA CGACATTTGT AATTGCCAGT GAAGGATTTA GCAATCGAAC TTTTGAGTAC 
ATGGTCACTG GCATTGTGGA GAGCGTTGAG ATAGCACACA AAAATATGAA ACCAGGCAAA 
ATCTTTATCA ATAAAGGGAC TGTGGATGGT GCACAGATCA ACCGAAGTCC TACTTCTTAC 
CTTTGGAATC CACAGTCAGA GAGAGCAAGG TATTCTTCAA ATACAGACAA GGAAATGGTG 
CTCCTGAAAA TGGTAGATCT GAATGGAGAA GAGCTGGGTC TTATCAGCTG GTTTGCCATC 
CACCCAGTCA GCATGAAAAA CACCAATCAT CTTGTAAATA GTGACAACGT AGGTTATGCA 
TCTTACCTTT TTGAGCAAGA GAAGAACAAA GGTTATCTAC CTGGAGAGGG ACCGTATGTA 
GCAGCTTTTG CTTCATCAAA TCTGGGAGAT GTTTCCCCCA ATGTTCTTGG CCCGCAATGC 
ACCAACACGG GAGAGTCTTG TGATAACGCC AATAGCTCTT GTCCCATTGG TGGGCCTAGC 
ATGTGTGTGG CTATGGGACC TGGACAGGAT ATGTTTGAAA GTACACAAAT CATAGGACGG 
ATAATATATG ACAAAGCAAA GGAACTGTAT GAGTCTGCCT CCCAGGAGGT AACTGGGCCA 
CTGGCTTCAG CTCATCAGTG GGTGAATATG ACGGATGTCA CCGTCTGGCT CAATTCAACA 
CACACAGTAA AAACGTGTAA ACCAGCCTTG GGCTACAGTT TTGCAGCTGG TACAATCGAT 
GGATTTGGAA CCGTCAGTTT TACTCAGGGG ATGACAGAAG GCGATTCATT TTGGGACACT 
CTTCGGGATC AGGTTCTGGG CAAGCCATCT GAAGAAATCA AAGAATGCCA TAAACCAAAG 
CCAGTCCTTC TTCAGACTGG AGAGCTGTTA AATCCTCATC CCTGGCACCC AGATATCGTT 
GACGTTCAGA TTGTTACTCT CGGGTTCTTG GCCATCACTG CCATCCCTGG GGAGTTCACG 
ACCATGTCTG GACGGAGACT TCGGCAGGCA GTTCGAGAAG AATTTGAAGC TTATGGGATG 
CTGAATATGA CTGTTGTTAT TTCAGGTCTA TGTAATGTAT ATACACACTA CATTACCACA 
TATGAAGAAT ACCAGGCGCA GCGGTACGAG GCCGCATCCA CAATTTATGG ACCGCATACT 
CTGTCTGCCT ACATCCAGCT TTTCAGAGTC CTTGCCAAGG CCATTGCTAC GGATACTGTA 
GCCAATTTGA GCAGTGGTCC AAAGCCTCCA TTTTTTGAAG AACTGATGTC TCCATTAATT 
CCAAATATTG TGGATAGAGC ACCTGAAGGC ACAACTTTTG GGGATGTCCT GCTACCAGCG 
AACACTATAT ACAGAGTGGG AGAAGTTGTT GAAGTTACCT TTGTAGGTGC TAACCCAAAG 
AATTCAGCAG AAAACCGGAC CCATCAGACC TTCCTCACTG TGGAGAAATA TGAGGCCACA 
TCAGCAACAT GGCAGATAAT GCATAATGAT GCCTCCTGGG AGACTCGTTT CTACTGGCAC 
AAAGGCTCAC TGGGTCTTAG CAATGCGACG ATACAGTGGC ACATTCCAGA TACTGCCCAG 
CCTGGAACCT ACAGAATAAA ATACTTTGGA CACAGTCGGA AGCAGGACTT ACTCAAGCCC 
GCTGTTCTAC TTTCATTTGA AAGCACTCCC TCTGTTTTTG AAGTCACAAC TACTTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ASAH2 ( XM_015469495.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015324981.1 Bos taurus N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2 (ASAH2), mRNA.

Price & Availability↑

CloneID	OBa88642
Clone ID Related Accession (Same CDS sequence)	XM_015469495.1 , XM_015460595.1
Accession Version	XM_015469495.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2340bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neutral ceramidase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646664.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAC GCACCTTTTC CAGCTTTGAA ATATTCCTGA TTTTCCTCCT TGTGATAACG 
ACAGGGATCA CGGTGGCTCT TCTCAGCCTG TTGTTTATCA CCAGTGGGAC CATTGAAAAT 
CACACAGGCA AGTATCGTTT TCCATCCACC CAGGGCCCTC CAGCTACCCA GAGCCCTCCA 
GCTACCCAGG GCTCTGCAGC CCCTCAAAAC TCAACACCCT CCCGGGGCCC CACAACTACC 
CAGGCATCTC CAACACCCAG CACCCCAGAG CCTACCCTGT TTCAGAACTT CAGTGGCTAC 
CATATTGGTG TTGGGCGAGC TGACTGCACA GGACAAGTAT CAGATATTAA TTTGATGGGC 
TACGCCAAAA CTGGCCAGTA TGCACGGGGC ATCCTCACGA GGTTGTACAG TCGTGCGTTC 
ATCATGGCGG AGCCGGATGG GTCCAATCGC ATAGTGTTTG TCAGCATCGA CATAGGCATG 
GTATCCCAGC GACTCAGGCT GGAGGTCCTG AGCAGGCTGC AGAATAAATA CGGCTCCCTG 
TACAGAAGAG ACAATGTTAT TCTGAGTGGC ACTCATACAC ACTCGGGTCC AGCAGGATAT 
TTCCAGTACA CGACATTTGT AATTGCCAGT GAAGGATTTA GCAATCGAAC TTTTGAGTAC 
ATGGTCACTG GCATTGTGGA GAGCGTTGAG ATAGCACACA AAAATATGAA ACCAGGCAAA 
ATCTTTATCA ATAAAGGGAC TGTGGATGGT GCACAGATCA ACCGAAGTCC TACTTCTTAC 
CTTTGGAATC CACAGTCAGA GAGAGCAAGG TATTCTTCAA ATACAGACAA GGAAATGGTG 
CTCCTGAAAA TGGTAGATCT GAATGGAGAA GAGCTGGGTC TTATCAGCTG GTTTGCCATC 
CACCCAGTCA GCATGAAAAA CACCAATCAT CTTGTAAATA GTGACAACGT AGGTTATGCA 
TCTTACCTTT TTGAGCAAGA GAAGAACAAA GGTTATCTAC CTGGAGAGGG ACCGTATGTA 
GCAGCTTTTG CTTCATCAAA TCTGGGAGAT GTTTCCCCCA ATGTTCTTGG CCCGCAATGC 
ACCAACACGG GAGAGTCTTG TGATAACGCC AATAGCTCTT GTCCCATTGG TGGGCCTAGC 
ATGTGTGTGG CTATGGGACC TGGGCAGGAT ATGTTTGAAA GTACACAAAT CATAGGACGG 
ATAATATATG ACAAAGCAAA GGAACTGTAT GAGTCTGCCT CCCAGGAGGT AACTGGGCCA 
CTGGCTTCAG CTCATCAGTG GGTGAATATG ACGGATGTCA CCGTCTGGCT CAATTCAACA 
CACACAGTAA AAACGTGTAA ACCAGCCTTG GGCTACAGTT TTGCAGCTGG TACAATCGAT 
GGATTTGGAA CCGTCAGTTT TACTCAGGGG ATGACAGAAG GCGATTCATT TTGGGACACT 
CTTCGGGATC AGGTTCTGGG CAAACCATCT GAAGAAATCA AAGAATGCCA TAAACCAAAG 
CCAGTCCTTC TTCAGACTGG AGAGCTGTTA AATCCTCATC CCTGGCACCC AGATATCGTT 
GACGTTCAGA TTGTTACTCT CGGGTTCTTG GCCATCACTG CCATCCCTGG GGAGTTCACG 
ACCATGTCTG GACGGAGACT TCGGCAGGCA GTTCGAGAAG AATTTGAAGC TTATGGGATG 
CTGAATATGA CTGTTGTTAT TTCAGGTCTA TGTAATGTAT ATACACACTA CATTACCACA 
TATGAAGAAT ACCAGGCGCA GCGGTACGAG GCCGCATCCA CAATTTATGG ACCGCATACT 
CTGTCTGCCT ACATCCAGCT TTTCAGAGTC CTTGCCAAGG CCATTGCTAC GGATACTGTA 
GCCAATTTGA GCAGTGGTCC AAAGCCTCCA TTTTTTGAAG AACTGATGTC TCCATTAATT 
CCAAATATTG TGGATAGAGC ACCTGAAGGC ACAACTTTTG GGGATGTCCT GCTACCAGCG 
AACACTATAT ACAGAGTGGG AGAAGTTGTT GAAGTTACCT TTGTAGGTGC TAACCCAAAG 
AATTCAGCAG AAAACCGGAC CCATCAGACC TTCCTCACTG TGGAGAAATA TGAGGCCACA 
TCAGCAACAT GGCAGATAAT GCATAATGAT GCCTCCTGGG AGACTCGTTT CTACTGGCAC 
AAAGGCTCAC TGGGTCTTAG CAATGCGACG ATACAGTGGC ACATTCCAGA TACTGCCCAG 
CCTGGAACCT ACAGAATAAA ATACTTTGGA CACAGTCGGA AGCAGGACTT ACTCAAGCCC 
GCTGTTCTAC TTTCATTTGA AAGCACTCCC TCTGTTTTTG AAGTCACAAC TACTTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015324981.1
CDS	201..2540
Translation	MAKRTFSSFEIFLIFLLVITTGITVALLSLLFITSGTIENHTGKYRFPSTQGPPATQSPPATQGSAAPQNSTPSRGPTTTQASPTPSTPEPTLFQNFSGYHIGVGRADCTGQVSDINLMGYAKTGQYARGILTRLYSRAFIMAEPDGSNRIVFVSIDIGMVSQRLRLEVLSRLQNKYGSLYRRDNVILSGTHTHSGPAGYFQYTTFVIASEGFSNRTFEYMVTGIVESVEIAHKNMKPGKIFINKGTVDGAQINRSPTSYLWNPQSERARYSSNTDKEMVLLKMVDLNGEELGLISWFAIHPVSMKNTNHLVNSDNVGYASYLFEQEKNKGYLPGEGPYVAAFASSNLGDVSPNVLGPQCTNTGESCDNANSSCPIGGPSMCVAMGPGQDMFESTQIIGRIIYDKAKELYESASQEVTGPLASAHQWVNMTDVTVWLNSTHTVKTCKPALGYSFAAGTIDGFGTVSFTQGMTEGDSFWDTLRDQVLGKPSEEIKECHKPKPVLLQTGELLNPHPWHPDIVDVQIVTLGFLAITAIPGEFTTMSGRRLRQAVREEFEAYGMLNMTVVISGLCNVYTHYITTYEEYQAQRYEAASTIYGPHTLSAYIQLFRVLAKAIATDTVANLSSGPKPPFFEELMSPLIPNIVDRAPEGTTFGDVLLPANTIYRVGEVVEVTFVGANPKNSAENRTHQTFLTVEKYEATSATWQIMHNDASWETRFYWHKGSLGLSNATIQWHIPDTAQPGTYRIKYFGHSRKQDLLKPAVLLSFESTPSVFEVTTTW

Target ORF information:

RefSeq Version	XM_015469495.1
Organism	Bos taurus(cattle)
Definition	Bos taurus N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2 (ASAH2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015469495.1

ORF Insert Sequence:

ATGGCCAAAC GCACCTTTTC CAGCTTTGAA ATATTCCTGA TTTTCCTCCT TGTGATAACG 
ACAGGGATCA CGGTGGCTCT TCTCAGCCTG TTGTTTATCA CCAGTGGGAC CATTGAAAAT 
CACACAGGCA AGTATCGTTT TCCATCCACC CAGGGCCCTC CAGCTACCCA GAGCCCTCCA 
GCTACCCAGG GCTCTGCAGC CCCTCAAAAC TCAACACCCT CCCGGGGCCC CACAACTACC 
CAGGCATCTC CAACACCCAG CACCCCAGAG CCTACCCTGT TTCAGAACTT CAGTGGCTAC 
CATATTGGTG TTGGGCGAGC TGACTGCACA GGACAAGTAT CAGATATTAA TTTGATGGGC 
TACGCCAAAA CTGGCCAGTA TGCACGGGGC ATCCTCACGA GGTTGTACAG TCGTGCGTTC 
ATCATGGCGG AGCCGGATGG GTCCAATCGC ATAGTGTTTG TCAGCATCGA CATAGGCATG 
GTATCCCAGC GACTCAGGCT GGAGGTCCTG AGCAGGCTGC AGAATAAATA CGGCTCCCTG 
TACAGAAGAG ACAATGTTAT TCTGAGTGGC ACTCATACAC ACTCGGGTCC AGCAGGATAT 
TTCCAGTACA CGACATTTGT AATTGCCAGT GAAGGATTTA GCAATCGAAC TTTTGAGTAC 
ATGGTCACTG GCATTGTGGA GAGCGTTGAG ATAGCACACA AAAATATGAA ACCAGGCAAA 
ATCTTTATCA ATAAAGGGAC TGTGGATGGT GCACAGATCA ACCGAAGTCC TACTTCTTAC 
CTTTGGAATC CACAGTCAGA GAGAGCAAGG TATTCTTCAA ATACAGACAA GGAAATGGTG 
CTCCTGAAAA TGGTAGATCT GAATGGAGAA GAGCTGGGTC TTATCAGCTG GTTTGCCATC 
CACCCAGTCA GCATGAAAAA CACCAATCAT CTTGTAAATA GTGACAACGT AGGTTATGCA 
TCTTACCTTT TTGAGCAAGA GAAGAACAAA GGTTATCTAC CTGGAGAGGG ACCGTATGTA 
GCAGCTTTTG CTTCATCAAA TCTGGGAGAT GTTTCCCCCA ATGTTCTTGG CCCGCAATGC 
ACCAACACGG GAGAGTCTTG TGATAACGCC AATAGCTCTT GTCCCATTGG TGGGCCTAGC 
ATGTGTGTGG CTATGGGACC TGGGCAGGAT ATGTTTGAAA GTACACAAAT CATAGGACGG 
ATAATATATG ACAAAGCAAA GGAACTGTAT GAGTCTGCCT CCCAGGAGGT AACTGGGCCA 
CTGGCTTCAG CTCATCAGTG GGTGAATATG ACGGATGTCA CCGTCTGGCT CAATTCAACA 
CACACAGTAA AAACGTGTAA ACCAGCCTTG GGCTACAGTT TTGCAGCTGG TACAATCGAT 
GGATTTGGAA CCGTCAGTTT TACTCAGGGG ATGACAGAAG GCGATTCATT TTGGGACACT 
CTTCGGGATC AGGTTCTGGG CAAACCATCT GAAGAAATCA AAGAATGCCA TAAACCAAAG 
CCAGTCCTTC TTCAGACTGG AGAGCTGTTA AATCCTCATC CCTGGCACCC AGATATCGTT 
GACGTTCAGA TTGTTACTCT CGGGTTCTTG GCCATCACTG CCATCCCTGG GGAGTTCACG 
ACCATGTCTG GACGGAGACT TCGGCAGGCA GTTCGAGAAG AATTTGAAGC TTATGGGATG 
CTGAATATGA CTGTTGTTAT TTCAGGTCTA TGTAATGTAT ATACACACTA CATTACCACA 
TATGAAGAAT ACCAGGCGCA GCGGTACGAG GCCGCATCCA CAATTTATGG ACCGCATACT 
CTGTCTGCCT ACATCCAGCT TTTCAGAGTC CTTGCCAAGG CCATTGCTAC GGATACTGTA 
GCCAATTTGA GCAGTGGTCC AAAGCCTCCA TTTTTTGAAG AACTGATGTC TCCATTAATT 
CCAAATATTG TGGATAGAGC ACCTGAAGGC ACAACTTTTG GGGATGTCCT GCTACCAGCG 
AACACTATAT ACAGAGTGGG AGAAGTTGTT GAAGTTACCT TTGTAGGTGC TAACCCAAAG 
AATTCAGCAG AAAACCGGAC CCATCAGACC TTCCTCACTG TGGAGAAATA TGAGGCCACA 
TCAGCAACAT GGCAGATAAT GCATAATGAT GCCTCCTGGG AGACTCGTTT CTACTGGCAC 
AAAGGCTCAC TGGGTCTTAG CAATGCGACG ATACAGTGGC ACATTCCAGA TACTGCCCAG 
CCTGGAACCT ACAGAATAAA ATACTTTGGA CACAGTCGGA AGCAGGACTT ACTCAAGCCC 
GCTGTTCTAC TTTCATTTGA AAGCACTCCC TCTGTTTTTG AAGTCACAAC TACTTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ASAH2 ( XM_015460595.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015316081.1 Bos taurus N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2 (ASAH2), mRNA.

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CloneID	OBa88642
Clone ID Related Accession (Same CDS sequence)	XM_015469495.1 , XM_015460595.1
Accession Version	XM_015460595.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2340bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neutral ceramidase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104580.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAC GCACCTTTTC CAGCTTTGAA ATATTCCTGA TTTTCCTCCT TGTGATAACG 
ACAGGGATCA CGGTGGCTCT TCTCAGCCTG TTGTTTATCA CCAGTGGGAC CATTGAAAAT 
CACACAGGCA AGTATCGTTT TCCATCCACC CAGGGCCCTC CAGCTACCCA GAGCCCTCCA 
GCTACCCAGG GCTCTGCAGC CCCTCAAAAC TCAACACCCT CCCGGGGCCC CACAACTACC 
CAGGCATCTC CAACACCCAG CACCCCAGAG CCTACCCTGT TTCAGAACTT CAGTGGCTAC 
CATATTGGTG TTGGGCGAGC TGACTGCACA GGACAAGTAT CAGATATTAA TTTGATGGGC 
TACGCCAAAA CTGGCCAGTA TGCACGGGGC ATCCTCACGA GGTTGTACAG TCGTGCGTTC 
ATCATGGCGG AGCCGGATGG GTCCAATCGC ATAGTGTTTG TCAGCATCGA CATAGGCATG 
GTATCCCAGC GACTCAGGCT GGAGGTCCTG AGCAGGCTGC AGAATAAATA CGGCTCCCTG 
TACAGAAGAG ACAATGTTAT TCTGAGTGGC ACTCATACAC ACTCGGGTCC AGCAGGATAT 
TTCCAGTACA CGACATTTGT AATTGCCAGT GAAGGATTTA GCAATCGAAC TTTTGAGTAC 
ATGGTCACTG GCATTGTGGA GAGCGTTGAG ATAGCACACA AAAATATGAA ACCAGGCAAA 
ATCTTTATCA ATAAAGGGAC TGTGGATGGT GCACAGATCA ACCGAAGTCC TACTTCTTAC 
CTTTGGAATC CACAGTCAGA GAGAGCAAGG TATTCTTCAA ATACAGACAA GGAAATGGTG 
CTCCTGAAAA TGGTAGATCT GAATGGAGAA GAGCTGGGTC TTATCAGCTG GTTTGCCATC 
CACCCAGTCA GCATGAAAAA CACCAATCAT CTTGTAAATA GTGACAACGT AGGTTATGCA 
TCTTACCTTT TTGAGCAAGA GAAGAACAAA GGTTATCTAC CTGGAGAGGG ACCGTATGTA 
GCAGCTTTTG CTTCATCAAA TCTGGGAGAT GTTTCCCCCA ATGTTCTTGG CCCGCAATGC 
ACCAACACGG GAGAGTCTTG TGATAACGCC AATAGCTCTT GTCCCATTGG TGGGCCTAGC 
ATGTGTGTGG CTATGGGACC TGGGCAGGAT ATGTTTGAAA GTACACAAAT CATAGGACGG 
ATAATATATG ACAAAGCAAA GGAACTGTAT GAGTCTGCCT CCCAGGAGGT AACTGGGCCA 
CTGGCTTCAG CTCATCAGTG GGTGAATATG ACGGATGTCA CCGTCTGGCT CAATTCAACA 
CACACAGTAA AAACGTGTAA ACCAGCCTTG GGCTACAGTT TTGCAGCTGG TACAATCGAT 
GGATTTGGAA CCGTCAGTTT TACTCAGGGG ATGACAGAAG GCGATTCATT TTGGGACACT 
CTTCGGGATC AGGTTCTGGG CAAACCATCT GAAGAAATCA AAGAATGCCA TAAACCAAAG 
CCAGTCCTTC TTCAGACTGG AGAGCTGTTA AATCCTCATC CCTGGCACCC AGATATCGTT 
GACGTTCAGA TTGTTACTCT CGGGTTCTTG GCCATCACTG CCATCCCTGG GGAGTTCACG 
ACCATGTCTG GACGGAGACT TCGGCAGGCA GTTCGAGAAG AATTTGAAGC TTATGGGATG 
CTGAATATGA CTGTTGTTAT TTCAGGTCTA TGTAATGTAT ATACACACTA CATTACCACA 
TATGAAGAAT ACCAGGCGCA GCGGTACGAG GCCGCATCCA CAATTTATGG ACCGCATACT 
CTGTCTGCCT ACATCCAGCT TTTCAGAGTC CTTGCCAAGG CCATTGCTAC GGATACTGTA 
GCCAATTTGA GCAGTGGTCC AAAGCCTCCA TTTTTTGAAG AACTGATGTC TCCATTAATT 
CCAAATATTG TGGATAGAGC ACCTGAAGGC ACAACTTTTG GGGATGTCCT GCTACCAGCG 
AACACTATAT ACAGAGTGGG AGAAGTTGTT GAAGTTACCT TTGTAGGTGC TAACCCAAAG 
AATTCAGCAG AAAACCGGAC CCATCAGACC TTCCTCACTG TGGAGAAATA TGAGGCCACA 
TCAGCAACAT GGCAGATAAT GCATAATGAT GCCTCCTGGG AGACTCGTTT CTACTGGCAC 
AAAGGCTCAC TGGGTCTTAG CAATGCGACG ATACAGTGGC ACATTCCAGA TACTGCCCAG 
CCTGGAACCT ACAGAATAAA ATACTTTGGA CACAGTCGGA AGCAGGACTT ACTCAAGCCC 
GCTGTTCTAC TTTCATTTGA AAGCACTCCC TCTGTTTTTG AAGTCACAAC TACTTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015316081.1
CDS	201..2540
Translation	MAKRTFSSFEIFLIFLLVITTGITVALLSLLFITSGTIENHTGKYRFPSTQGPPATQSPPATQGSAAPQNSTPSRGPTTTQASPTPSTPEPTLFQNFSGYHIGVGRADCTGQVSDINLMGYAKTGQYARGILTRLYSRAFIMAEPDGSNRIVFVSIDIGMVSQRLRLEVLSRLQNKYGSLYRRDNVILSGTHTHSGPAGYFQYTTFVIASEGFSNRTFEYMVTGIVESVEIAHKNMKPGKIFINKGTVDGAQINRSPTSYLWNPQSERARYSSNTDKEMVLLKMVDLNGEELGLISWFAIHPVSMKNTNHLVNSDNVGYASYLFEQEKNKGYLPGEGPYVAAFASSNLGDVSPNVLGPQCTNTGESCDNANSSCPIGGPSMCVAMGPGQDMFESTQIIGRIIYDKAKELYESASQEVTGPLASAHQWVNMTDVTVWLNSTHTVKTCKPALGYSFAAGTIDGFGTVSFTQGMTEGDSFWDTLRDQVLGKPSEEIKECHKPKPVLLQTGELLNPHPWHPDIVDVQIVTLGFLAITAIPGEFTTMSGRRLRQAVREEFEAYGMLNMTVVISGLCNVYTHYITTYEEYQAQRYEAASTIYGPHTLSAYIQLFRVLAKAIATDTVANLSSGPKPPFFEELMSPLIPNIVDRAPEGTTFGDVLLPANTIYRVGEVVEVTFVGANPKNSAENRTHQTFLTVEKYEATSATWQIMHNDASWETRFYWHKGSLGLSNATIQWHIPDTAQPGTYRIKYFGHSRKQDLLKPAVLLSFESTPSVFEVTTTW

Target ORF information:

RefSeq Version	XM_015460595.1
Organism	Bos taurus(cattle)
Definition	Bos taurus N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2 (ASAH2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015460595.1

ORF Insert Sequence:

ATGGCCAAAC GCACCTTTTC CAGCTTTGAA ATATTCCTGA TTTTCCTCCT TGTGATAACG 
ACAGGGATCA CGGTGGCTCT TCTCAGCCTG TTGTTTATCA CCAGTGGGAC CATTGAAAAT 
CACACAGGCA AGTATCGTTT TCCATCCACC CAGGGCCCTC CAGCTACCCA GAGCCCTCCA 
GCTACCCAGG GCTCTGCAGC CCCTCAAAAC TCAACACCCT CCCGGGGCCC CACAACTACC 
CAGGCATCTC CAACACCCAG CACCCCAGAG CCTACCCTGT TTCAGAACTT CAGTGGCTAC 
CATATTGGTG TTGGGCGAGC TGACTGCACA GGACAAGTAT CAGATATTAA TTTGATGGGC 
TACGCCAAAA CTGGCCAGTA TGCACGGGGC ATCCTCACGA GGTTGTACAG TCGTGCGTTC 
ATCATGGCGG AGCCGGATGG GTCCAATCGC ATAGTGTTTG TCAGCATCGA CATAGGCATG 
GTATCCCAGC GACTCAGGCT GGAGGTCCTG AGCAGGCTGC AGAATAAATA CGGCTCCCTG 
TACAGAAGAG ACAATGTTAT TCTGAGTGGC ACTCATACAC ACTCGGGTCC AGCAGGATAT 
TTCCAGTACA CGACATTTGT AATTGCCAGT GAAGGATTTA GCAATCGAAC TTTTGAGTAC 
ATGGTCACTG GCATTGTGGA GAGCGTTGAG ATAGCACACA AAAATATGAA ACCAGGCAAA 
ATCTTTATCA ATAAAGGGAC TGTGGATGGT GCACAGATCA ACCGAAGTCC TACTTCTTAC 
CTTTGGAATC CACAGTCAGA GAGAGCAAGG TATTCTTCAA ATACAGACAA GGAAATGGTG 
CTCCTGAAAA TGGTAGATCT GAATGGAGAA GAGCTGGGTC TTATCAGCTG GTTTGCCATC 
CACCCAGTCA GCATGAAAAA CACCAATCAT CTTGTAAATA GTGACAACGT AGGTTATGCA 
TCTTACCTTT TTGAGCAAGA GAAGAACAAA GGTTATCTAC CTGGAGAGGG ACCGTATGTA 
GCAGCTTTTG CTTCATCAAA TCTGGGAGAT GTTTCCCCCA ATGTTCTTGG CCCGCAATGC 
ACCAACACGG GAGAGTCTTG TGATAACGCC AATAGCTCTT GTCCCATTGG TGGGCCTAGC 
ATGTGTGTGG CTATGGGACC TGGGCAGGAT ATGTTTGAAA GTACACAAAT CATAGGACGG 
ATAATATATG ACAAAGCAAA GGAACTGTAT GAGTCTGCCT CCCAGGAGGT AACTGGGCCA 
CTGGCTTCAG CTCATCAGTG GGTGAATATG ACGGATGTCA CCGTCTGGCT CAATTCAACA 
CACACAGTAA AAACGTGTAA ACCAGCCTTG GGCTACAGTT TTGCAGCTGG TACAATCGAT 
GGATTTGGAA CCGTCAGTTT TACTCAGGGG ATGACAGAAG GCGATTCATT TTGGGACACT 
CTTCGGGATC AGGTTCTGGG CAAACCATCT GAAGAAATCA AAGAATGCCA TAAACCAAAG 
CCAGTCCTTC TTCAGACTGG AGAGCTGTTA AATCCTCATC CCTGGCACCC AGATATCGTT 
GACGTTCAGA TTGTTACTCT CGGGTTCTTG GCCATCACTG CCATCCCTGG GGAGTTCACG 
ACCATGTCTG GACGGAGACT TCGGCAGGCA GTTCGAGAAG AATTTGAAGC TTATGGGATG 
CTGAATATGA CTGTTGTTAT TTCAGGTCTA TGTAATGTAT ATACACACTA CATTACCACA 
TATGAAGAAT ACCAGGCGCA GCGGTACGAG GCCGCATCCA CAATTTATGG ACCGCATACT 
CTGTCTGCCT ACATCCAGCT TTTCAGAGTC CTTGCCAAGG CCATTGCTAC GGATACTGTA 
GCCAATTTGA GCAGTGGTCC AAAGCCTCCA TTTTTTGAAG AACTGATGTC TCCATTAATT 
CCAAATATTG TGGATAGAGC ACCTGAAGGC ACAACTTTTG GGGATGTCCT GCTACCAGCG 
AACACTATAT ACAGAGTGGG AGAAGTTGTT GAAGTTACCT TTGTAGGTGC TAACCCAAAG 
AATTCAGCAG AAAACCGGAC CCATCAGACC TTCCTCACTG TGGAGAAATA TGAGGCCACA 
TCAGCAACAT GGCAGATAAT GCATAATGAT GCCTCCTGGG AGACTCGTTT CTACTGGCAC 
AAAGGCTCAC TGGGTCTTAG CAATGCGACG ATACAGTGGC ACATTCCAGA TACTGCCCAG 
CCTGGAACCT ACAGAATAAA ATACTTTGGA CACAGTCGGA AGCAGGACTT ACTCAAGCCC 
GCTGTTCTAC TTTCATTTGA AAGCACTCCC TCTGTTTTTG AAGTCACAAC TACTTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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ASAH2 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

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FAX

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