ASAH2 cDNA ORF clone, Bos taurus(cattle)

The following ASAH2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ASAH2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa231132 XM_015460595.2
Latest version!
Bos taurus N-acylsphingosine amidohydrolase 2 (ASAH2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa88642 XM_015469495.1
Latest version!
Bos taurus N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2 (ASAH2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OBa88642 XM_015460595.1
Latest version!
Bos taurus N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2 (ASAH2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa231132
    Clone ID Related Accession (Same CDS sequence) XM_015460595.2
    Accession Version XM_015460595.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2340bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product putative neutral ceramidase C
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037353.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015460595.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCCAAAC GCACCTTTTC CAGCTTTGAA ATATTCCTGA TTTTCCTCCT TGTGATAACG 
    ACAGGGATCA CGGTGGCTCT TCTCAGCCTG TTGTTTATCA CCAGTGGGAC CATTGAAAAT
    CACACAGGCA AGTATCGTTT TCCATCCACC CAGGGCCCTC CAGCTACCCA GAGCCCTCCA
    GCTACCCAGG GCTCTGCAGC CCCTCAAAAC TCAACACCCT CCCGGGGCCC CACAACTACC
    CAGGCATCTC CAACACCCAG CACCCCAGAG CCTACCCTGT TTCAGAACTT CAGTGGCTAC
    CATATTGGTG TTGGGCGAGC TGACTGCACA GGACAAGTAT CAGATATTAA TTTGATGGGC
    TACGCCAAAA CTGGCCAGTA TGCACGGGGC ATCCTCACGA GGTTGTACAG TCGTGCGTTC
    ATCATGGCGG AGCCGGATGG GTCCAATCGC ATAGTGTTTG TCAGCATCGA CATAGGCATG
    GTATCCCAGC GACTCAGGCT GGAGGTCCTG AGCAGGCTGC AGAATAAATA CGGCTCCCTG
    TACAGAAGAG ACAATGTTAT TCTGAGTGGC ACTCATACAC ACTCGGGTCC AGCAGGATAT
    TTCCAGTACA CGACATTTGT AATTGCCAGT GAAGGATTTA GCAATCGAAC TTTTGAGTAC
    ATGGTCACTG GCATTGTGGA GAGCGTTGAG ATAGCACACA AAAATATGAA ACCAGGCAAA
    ATCTTTATCA ATAAAGGGAC TGTGGATGGT GCACAGATCA ACCGAAGTCC TACTTCTTAC
    CTTTGGAATC CACAGTCAGA GAGAGCAAGG TATTCTTCAA ATACAGACAA GGAAATGGTG
    CTCCTGAAAA TGGTAGATCT GAATGGAGAA GAGCTGGGTC TTATCAGCTG GTTTGCCATC
    CACCCAGTCA GCATGAAAAA CACCAATCAT CTTGTAAATA GTGACAACGT AGGTTATGCA
    TCTTACCTTT TTGAGCAAGA GAAGAACAAA GGTTATCTAC CTGGAGAGGG ACCGTATGTA
    GCAGCTTTTG CTTCATCAAA TCTGGGAGAT GTTTCCCCCA ATGTTCTTGG CCCGCAATGC
    ACCAACACGG GAGAGTCTTG TGATAACGCC AATAGCTCTT GTCCCATTGG TGGGCCTAGC
    ATGTGTGTGG CTATGGGACC TGGACAGGAT ATGTTTGAAA GTACACAAAT CATAGGACGG
    ATAATATATG ACAAAGCAAA GGAACTGTAT GAGTCTGCCT CCCAGGAGGT AACTGGGCCA
    CTGGCTTCAG CTCATCAGTG GGTGAATATG ACGGATGTCA CCGTCTGGCT CAATTCAACA
    CACACAGTAA AAACGTGTAA ACCAGCCTTG GGCTACAGTT TTGCAGCTGG TACAATCGAT
    GGATTTGGAA CCGTCAGTTT TACTCAGGGG ATGACAGAAG GCGATTCATT TTGGGACACT
    CTTCGGGATC AGGTTCTGGG CAAGCCATCT GAAGAAATCA AAGAATGCCA TAAACCAAAG
    CCAGTCCTTC TTCAGACTGG AGAGCTGTTA AATCCTCATC CCTGGCACCC AGATATCGTT
    GACGTTCAGA TTGTTACTCT CGGGTTCTTG GCCATCACTG CCATCCCTGG GGAGTTCACG
    ACCATGTCTG GACGGAGACT TCGGCAGGCA GTTCGAGAAG AATTTGAAGC TTATGGGATG
    CTGAATATGA CTGTTGTTAT TTCAGGTCTA TGTAATGTAT ATACACACTA CATTACCACA
    TATGAAGAAT ACCAGGCGCA GCGGTACGAG GCCGCATCCA CAATTTATGG ACCGCATACT
    CTGTCTGCCT ACATCCAGCT TTTCAGAGTC CTTGCCAAGG CCATTGCTAC GGATACTGTA
    GCCAATTTGA GCAGTGGTCC AAAGCCTCCA TTTTTTGAAG AACTGATGTC TCCATTAATT
    CCAAATATTG TGGATAGAGC ACCTGAAGGC ACAACTTTTG GGGATGTCCT GCTACCAGCG
    AACACTATAT ACAGAGTGGG AGAAGTTGTT GAAGTTACCT TTGTAGGTGC TAACCCAAAG
    AATTCAGCAG AAAACCGGAC CCATCAGACC TTCCTCACTG TGGAGAAATA TGAGGCCACA
    TCAGCAACAT GGCAGATAAT GCATAATGAT GCCTCCTGGG AGACTCGTTT CTACTGGCAC
    AAAGGCTCAC TGGGTCTTAG CAATGCGACG ATACAGTGGC ACATTCCAGA TACTGCCCAG
    CCTGGAACCT ACAGAATAAA ATACTTTGGA CACAGTCGGA AGCAGGACTT ACTCAAGCCC
    GCTGTTCTAC TTTCATTTGA AAGCACTCCC TCTGTTTTTG AAGTCACAAC TACTTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015316081.1
    CDS169..2508
    Translation

    Target ORF information:

    RefSeq Version XM_015460595.2
    Organism Bos taurus(cattle)
    Definition Bos taurus N-acylsphingosine amidohydrolase 2 (ASAH2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015460595.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCCAAAC GCACCTTTTC CAGCTTTGAA ATATTCCTGA TTTTCCTCCT TGTGATAACG 
    ACAGGGATCA CGGTGGCTCT TCTCAGCCTG TTGTTTATCA CCAGTGGGAC CATTGAAAAT
    CACACAGGCA AGTATCGTTT TCCATCCACC CAGGGCCCTC CAGCTACCCA GAGCCCTCCA
    GCTACCCAGG GCTCTGCAGC CCCTCAAAAC TCAACACCCT CCCGGGGCCC CACAACTACC
    CAGGCATCTC CAACACCCAG CACCCCAGAG CCTACCCTGT TTCAGAACTT CAGTGGCTAC
    CATATTGGTG TTGGGCGAGC TGACTGCACA GGACAAGTAT CAGATATTAA TTTGATGGGC
    TACGCCAAAA CTGGCCAGTA TGCACGGGGC ATCCTCACGA GGTTGTACAG TCGTGCGTTC
    ATCATGGCGG AGCCGGATGG GTCCAATCGC ATAGTGTTTG TCAGCATCGA CATAGGCATG
    GTATCCCAGC GACTCAGGCT GGAGGTCCTG AGCAGGCTGC AGAATAAATA CGGCTCCCTG
    TACAGAAGAG ACAATGTTAT TCTGAGTGGC ACTCATACAC ACTCGGGTCC AGCAGGATAT
    TTCCAGTACA CGACATTTGT AATTGCCAGT GAAGGATTTA GCAATCGAAC TTTTGAGTAC
    ATGGTCACTG GCATTGTGGA GAGCGTTGAG ATAGCACACA AAAATATGAA ACCAGGCAAA
    ATCTTTATCA ATAAAGGGAC TGTGGATGGT GCACAGATCA ACCGAAGTCC TACTTCTTAC
    CTTTGGAATC CACAGTCAGA GAGAGCAAGG TATTCTTCAA ATACAGACAA GGAAATGGTG
    CTCCTGAAAA TGGTAGATCT GAATGGAGAA GAGCTGGGTC TTATCAGCTG GTTTGCCATC
    CACCCAGTCA GCATGAAAAA CACCAATCAT CTTGTAAATA GTGACAACGT AGGTTATGCA
    TCTTACCTTT TTGAGCAAGA GAAGAACAAA GGTTATCTAC CTGGAGAGGG ACCGTATGTA
    GCAGCTTTTG CTTCATCAAA TCTGGGAGAT GTTTCCCCCA ATGTTCTTGG CCCGCAATGC
    ACCAACACGG GAGAGTCTTG TGATAACGCC AATAGCTCTT GTCCCATTGG TGGGCCTAGC
    ATGTGTGTGG CTATGGGACC TGGACAGGAT ATGTTTGAAA GTACACAAAT CATAGGACGG
    ATAATATATG ACAAAGCAAA GGAACTGTAT GAGTCTGCCT CCCAGGAGGT AACTGGGCCA
    CTGGCTTCAG CTCATCAGTG GGTGAATATG ACGGATGTCA CCGTCTGGCT CAATTCAACA
    CACACAGTAA AAACGTGTAA ACCAGCCTTG GGCTACAGTT TTGCAGCTGG TACAATCGAT
    GGATTTGGAA CCGTCAGTTT TACTCAGGGG ATGACAGAAG GCGATTCATT TTGGGACACT
    CTTCGGGATC AGGTTCTGGG CAAGCCATCT GAAGAAATCA AAGAATGCCA TAAACCAAAG
    CCAGTCCTTC TTCAGACTGG AGAGCTGTTA AATCCTCATC CCTGGCACCC AGATATCGTT
    GACGTTCAGA TTGTTACTCT CGGGTTCTTG GCCATCACTG CCATCCCTGG GGAGTTCACG
    ACCATGTCTG GACGGAGACT TCGGCAGGCA GTTCGAGAAG AATTTGAAGC TTATGGGATG
    CTGAATATGA CTGTTGTTAT TTCAGGTCTA TGTAATGTAT ATACACACTA CATTACCACA
    TATGAAGAAT ACCAGGCGCA GCGGTACGAG GCCGCATCCA CAATTTATGG ACCGCATACT
    CTGTCTGCCT ACATCCAGCT TTTCAGAGTC CTTGCCAAGG CCATTGCTAC GGATACTGTA
    GCCAATTTGA GCAGTGGTCC AAAGCCTCCA TTTTTTGAAG AACTGATGTC TCCATTAATT
    CCAAATATTG TGGATAGAGC ACCTGAAGGC ACAACTTTTG GGGATGTCCT GCTACCAGCG
    AACACTATAT ACAGAGTGGG AGAAGTTGTT GAAGTTACCT TTGTAGGTGC TAACCCAAAG
    AATTCAGCAG AAAACCGGAC CCATCAGACC TTCCTCACTG TGGAGAAATA TGAGGCCACA
    TCAGCAACAT GGCAGATAAT GCATAATGAT GCCTCCTGGG AGACTCGTTT CTACTGGCAC
    AAAGGCTCAC TGGGTCTTAG CAATGCGACG ATACAGTGGC ACATTCCAGA TACTGCCCAG
    CCTGGAACCT ACAGAATAAA ATACTTTGGA CACAGTCGGA AGCAGGACTT ACTCAAGCCC
    GCTGTTCTAC TTTCATTTGA AAGCACTCCC TCTGTTTTTG AAGTCACAAC TACTTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88642
    Clone ID Related Accession (Same CDS sequence) XM_015469495.1 , XM_015460595.1
    Accession Version XM_015469495.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2340bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product neutral ceramidase
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646664.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCCAAAC GCACCTTTTC CAGCTTTGAA ATATTCCTGA TTTTCCTCCT TGTGATAACG 
    ACAGGGATCA CGGTGGCTCT TCTCAGCCTG TTGTTTATCA CCAGTGGGAC CATTGAAAAT
    CACACAGGCA AGTATCGTTT TCCATCCACC CAGGGCCCTC CAGCTACCCA GAGCCCTCCA
    GCTACCCAGG GCTCTGCAGC CCCTCAAAAC TCAACACCCT CCCGGGGCCC CACAACTACC
    CAGGCATCTC CAACACCCAG CACCCCAGAG CCTACCCTGT TTCAGAACTT CAGTGGCTAC
    CATATTGGTG TTGGGCGAGC TGACTGCACA GGACAAGTAT CAGATATTAA TTTGATGGGC
    TACGCCAAAA CTGGCCAGTA TGCACGGGGC ATCCTCACGA GGTTGTACAG TCGTGCGTTC
    ATCATGGCGG AGCCGGATGG GTCCAATCGC ATAGTGTTTG TCAGCATCGA CATAGGCATG
    GTATCCCAGC GACTCAGGCT GGAGGTCCTG AGCAGGCTGC AGAATAAATA CGGCTCCCTG
    TACAGAAGAG ACAATGTTAT TCTGAGTGGC ACTCATACAC ACTCGGGTCC AGCAGGATAT
    TTCCAGTACA CGACATTTGT AATTGCCAGT GAAGGATTTA GCAATCGAAC TTTTGAGTAC
    ATGGTCACTG GCATTGTGGA GAGCGTTGAG ATAGCACACA AAAATATGAA ACCAGGCAAA
    ATCTTTATCA ATAAAGGGAC TGTGGATGGT GCACAGATCA ACCGAAGTCC TACTTCTTAC
    CTTTGGAATC CACAGTCAGA GAGAGCAAGG TATTCTTCAA ATACAGACAA GGAAATGGTG
    CTCCTGAAAA TGGTAGATCT GAATGGAGAA GAGCTGGGTC TTATCAGCTG GTTTGCCATC
    CACCCAGTCA GCATGAAAAA CACCAATCAT CTTGTAAATA GTGACAACGT AGGTTATGCA
    TCTTACCTTT TTGAGCAAGA GAAGAACAAA GGTTATCTAC CTGGAGAGGG ACCGTATGTA
    GCAGCTTTTG CTTCATCAAA TCTGGGAGAT GTTTCCCCCA ATGTTCTTGG CCCGCAATGC
    ACCAACACGG GAGAGTCTTG TGATAACGCC AATAGCTCTT GTCCCATTGG TGGGCCTAGC
    ATGTGTGTGG CTATGGGACC TGGGCAGGAT ATGTTTGAAA GTACACAAAT CATAGGACGG
    ATAATATATG ACAAAGCAAA GGAACTGTAT GAGTCTGCCT CCCAGGAGGT AACTGGGCCA
    CTGGCTTCAG CTCATCAGTG GGTGAATATG ACGGATGTCA CCGTCTGGCT CAATTCAACA
    CACACAGTAA AAACGTGTAA ACCAGCCTTG GGCTACAGTT TTGCAGCTGG TACAATCGAT
    GGATTTGGAA CCGTCAGTTT TACTCAGGGG ATGACAGAAG GCGATTCATT TTGGGACACT
    CTTCGGGATC AGGTTCTGGG CAAACCATCT GAAGAAATCA AAGAATGCCA TAAACCAAAG
    CCAGTCCTTC TTCAGACTGG AGAGCTGTTA AATCCTCATC CCTGGCACCC AGATATCGTT
    GACGTTCAGA TTGTTACTCT CGGGTTCTTG GCCATCACTG CCATCCCTGG GGAGTTCACG
    ACCATGTCTG GACGGAGACT TCGGCAGGCA GTTCGAGAAG AATTTGAAGC TTATGGGATG
    CTGAATATGA CTGTTGTTAT TTCAGGTCTA TGTAATGTAT ATACACACTA CATTACCACA
    TATGAAGAAT ACCAGGCGCA GCGGTACGAG GCCGCATCCA CAATTTATGG ACCGCATACT
    CTGTCTGCCT ACATCCAGCT TTTCAGAGTC CTTGCCAAGG CCATTGCTAC GGATACTGTA
    GCCAATTTGA GCAGTGGTCC AAAGCCTCCA TTTTTTGAAG AACTGATGTC TCCATTAATT
    CCAAATATTG TGGATAGAGC ACCTGAAGGC ACAACTTTTG GGGATGTCCT GCTACCAGCG
    AACACTATAT ACAGAGTGGG AGAAGTTGTT GAAGTTACCT TTGTAGGTGC TAACCCAAAG
    AATTCAGCAG AAAACCGGAC CCATCAGACC TTCCTCACTG TGGAGAAATA TGAGGCCACA
    TCAGCAACAT GGCAGATAAT GCATAATGAT GCCTCCTGGG AGACTCGTTT CTACTGGCAC
    AAAGGCTCAC TGGGTCTTAG CAATGCGACG ATACAGTGGC ACATTCCAGA TACTGCCCAG
    CCTGGAACCT ACAGAATAAA ATACTTTGGA CACAGTCGGA AGCAGGACTT ACTCAAGCCC
    GCTGTTCTAC TTTCATTTGA AAGCACTCCC TCTGTTTTTG AAGTCACAAC TACTTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015324981.1
    CDS201..2540
    Translation

    Target ORF information:

    RefSeq Version XM_015469495.1
    Organism Bos taurus(cattle)
    Definition Bos taurus N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2 (ASAH2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015469495.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCCAAAC GCACCTTTTC CAGCTTTGAA ATATTCCTGA TTTTCCTCCT TGTGATAACG 
    ACAGGGATCA CGGTGGCTCT TCTCAGCCTG TTGTTTATCA CCAGTGGGAC CATTGAAAAT
    CACACAGGCA AGTATCGTTT TCCATCCACC CAGGGCCCTC CAGCTACCCA GAGCCCTCCA
    GCTACCCAGG GCTCTGCAGC CCCTCAAAAC TCAACACCCT CCCGGGGCCC CACAACTACC
    CAGGCATCTC CAACACCCAG CACCCCAGAG CCTACCCTGT TTCAGAACTT CAGTGGCTAC
    CATATTGGTG TTGGGCGAGC TGACTGCACA GGACAAGTAT CAGATATTAA TTTGATGGGC
    TACGCCAAAA CTGGCCAGTA TGCACGGGGC ATCCTCACGA GGTTGTACAG TCGTGCGTTC
    ATCATGGCGG AGCCGGATGG GTCCAATCGC ATAGTGTTTG TCAGCATCGA CATAGGCATG
    GTATCCCAGC GACTCAGGCT GGAGGTCCTG AGCAGGCTGC AGAATAAATA CGGCTCCCTG
    TACAGAAGAG ACAATGTTAT TCTGAGTGGC ACTCATACAC ACTCGGGTCC AGCAGGATAT
    TTCCAGTACA CGACATTTGT AATTGCCAGT GAAGGATTTA GCAATCGAAC TTTTGAGTAC
    ATGGTCACTG GCATTGTGGA GAGCGTTGAG ATAGCACACA AAAATATGAA ACCAGGCAAA
    ATCTTTATCA ATAAAGGGAC TGTGGATGGT GCACAGATCA ACCGAAGTCC TACTTCTTAC
    CTTTGGAATC CACAGTCAGA GAGAGCAAGG TATTCTTCAA ATACAGACAA GGAAATGGTG
    CTCCTGAAAA TGGTAGATCT GAATGGAGAA GAGCTGGGTC TTATCAGCTG GTTTGCCATC
    CACCCAGTCA GCATGAAAAA CACCAATCAT CTTGTAAATA GTGACAACGT AGGTTATGCA
    TCTTACCTTT TTGAGCAAGA GAAGAACAAA GGTTATCTAC CTGGAGAGGG ACCGTATGTA
    GCAGCTTTTG CTTCATCAAA TCTGGGAGAT GTTTCCCCCA ATGTTCTTGG CCCGCAATGC
    ACCAACACGG GAGAGTCTTG TGATAACGCC AATAGCTCTT GTCCCATTGG TGGGCCTAGC
    ATGTGTGTGG CTATGGGACC TGGGCAGGAT ATGTTTGAAA GTACACAAAT CATAGGACGG
    ATAATATATG ACAAAGCAAA GGAACTGTAT GAGTCTGCCT CCCAGGAGGT AACTGGGCCA
    CTGGCTTCAG CTCATCAGTG GGTGAATATG ACGGATGTCA CCGTCTGGCT CAATTCAACA
    CACACAGTAA AAACGTGTAA ACCAGCCTTG GGCTACAGTT TTGCAGCTGG TACAATCGAT
    GGATTTGGAA CCGTCAGTTT TACTCAGGGG ATGACAGAAG GCGATTCATT TTGGGACACT
    CTTCGGGATC AGGTTCTGGG CAAACCATCT GAAGAAATCA AAGAATGCCA TAAACCAAAG
    CCAGTCCTTC TTCAGACTGG AGAGCTGTTA AATCCTCATC CCTGGCACCC AGATATCGTT
    GACGTTCAGA TTGTTACTCT CGGGTTCTTG GCCATCACTG CCATCCCTGG GGAGTTCACG
    ACCATGTCTG GACGGAGACT TCGGCAGGCA GTTCGAGAAG AATTTGAAGC TTATGGGATG
    CTGAATATGA CTGTTGTTAT TTCAGGTCTA TGTAATGTAT ATACACACTA CATTACCACA
    TATGAAGAAT ACCAGGCGCA GCGGTACGAG GCCGCATCCA CAATTTATGG ACCGCATACT
    CTGTCTGCCT ACATCCAGCT TTTCAGAGTC CTTGCCAAGG CCATTGCTAC GGATACTGTA
    GCCAATTTGA GCAGTGGTCC AAAGCCTCCA TTTTTTGAAG AACTGATGTC TCCATTAATT
    CCAAATATTG TGGATAGAGC ACCTGAAGGC ACAACTTTTG GGGATGTCCT GCTACCAGCG
    AACACTATAT ACAGAGTGGG AGAAGTTGTT GAAGTTACCT TTGTAGGTGC TAACCCAAAG
    AATTCAGCAG AAAACCGGAC CCATCAGACC TTCCTCACTG TGGAGAAATA TGAGGCCACA
    TCAGCAACAT GGCAGATAAT GCATAATGAT GCCTCCTGGG AGACTCGTTT CTACTGGCAC
    AAAGGCTCAC TGGGTCTTAG CAATGCGACG ATACAGTGGC ACATTCCAGA TACTGCCCAG
    CCTGGAACCT ACAGAATAAA ATACTTTGGA CACAGTCGGA AGCAGGACTT ACTCAAGCCC
    GCTGTTCTAC TTTCATTTGA AAGCACTCCC TCTGTTTTTG AAGTCACAAC TACTTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88642
    Clone ID Related Accession (Same CDS sequence) XM_015469495.1 , XM_015460595.1
    Accession Version XM_015460595.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2340bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product neutral ceramidase
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104580.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCCAAAC GCACCTTTTC CAGCTTTGAA ATATTCCTGA TTTTCCTCCT TGTGATAACG 
    ACAGGGATCA CGGTGGCTCT TCTCAGCCTG TTGTTTATCA CCAGTGGGAC CATTGAAAAT
    CACACAGGCA AGTATCGTTT TCCATCCACC CAGGGCCCTC CAGCTACCCA GAGCCCTCCA
    GCTACCCAGG GCTCTGCAGC CCCTCAAAAC TCAACACCCT CCCGGGGCCC CACAACTACC
    CAGGCATCTC CAACACCCAG CACCCCAGAG CCTACCCTGT TTCAGAACTT CAGTGGCTAC
    CATATTGGTG TTGGGCGAGC TGACTGCACA GGACAAGTAT CAGATATTAA TTTGATGGGC
    TACGCCAAAA CTGGCCAGTA TGCACGGGGC ATCCTCACGA GGTTGTACAG TCGTGCGTTC
    ATCATGGCGG AGCCGGATGG GTCCAATCGC ATAGTGTTTG TCAGCATCGA CATAGGCATG
    GTATCCCAGC GACTCAGGCT GGAGGTCCTG AGCAGGCTGC AGAATAAATA CGGCTCCCTG
    TACAGAAGAG ACAATGTTAT TCTGAGTGGC ACTCATACAC ACTCGGGTCC AGCAGGATAT
    TTCCAGTACA CGACATTTGT AATTGCCAGT GAAGGATTTA GCAATCGAAC TTTTGAGTAC
    ATGGTCACTG GCATTGTGGA GAGCGTTGAG ATAGCACACA AAAATATGAA ACCAGGCAAA
    ATCTTTATCA ATAAAGGGAC TGTGGATGGT GCACAGATCA ACCGAAGTCC TACTTCTTAC
    CTTTGGAATC CACAGTCAGA GAGAGCAAGG TATTCTTCAA ATACAGACAA GGAAATGGTG
    CTCCTGAAAA TGGTAGATCT GAATGGAGAA GAGCTGGGTC TTATCAGCTG GTTTGCCATC
    CACCCAGTCA GCATGAAAAA CACCAATCAT CTTGTAAATA GTGACAACGT AGGTTATGCA
    TCTTACCTTT TTGAGCAAGA GAAGAACAAA GGTTATCTAC CTGGAGAGGG ACCGTATGTA
    GCAGCTTTTG CTTCATCAAA TCTGGGAGAT GTTTCCCCCA ATGTTCTTGG CCCGCAATGC
    ACCAACACGG GAGAGTCTTG TGATAACGCC AATAGCTCTT GTCCCATTGG TGGGCCTAGC
    ATGTGTGTGG CTATGGGACC TGGGCAGGAT ATGTTTGAAA GTACACAAAT CATAGGACGG
    ATAATATATG ACAAAGCAAA GGAACTGTAT GAGTCTGCCT CCCAGGAGGT AACTGGGCCA
    CTGGCTTCAG CTCATCAGTG GGTGAATATG ACGGATGTCA CCGTCTGGCT CAATTCAACA
    CACACAGTAA AAACGTGTAA ACCAGCCTTG GGCTACAGTT TTGCAGCTGG TACAATCGAT
    GGATTTGGAA CCGTCAGTTT TACTCAGGGG ATGACAGAAG GCGATTCATT TTGGGACACT
    CTTCGGGATC AGGTTCTGGG CAAACCATCT GAAGAAATCA AAGAATGCCA TAAACCAAAG
    CCAGTCCTTC TTCAGACTGG AGAGCTGTTA AATCCTCATC CCTGGCACCC AGATATCGTT
    GACGTTCAGA TTGTTACTCT CGGGTTCTTG GCCATCACTG CCATCCCTGG GGAGTTCACG
    ACCATGTCTG GACGGAGACT TCGGCAGGCA GTTCGAGAAG AATTTGAAGC TTATGGGATG
    CTGAATATGA CTGTTGTTAT TTCAGGTCTA TGTAATGTAT ATACACACTA CATTACCACA
    TATGAAGAAT ACCAGGCGCA GCGGTACGAG GCCGCATCCA CAATTTATGG ACCGCATACT
    CTGTCTGCCT ACATCCAGCT TTTCAGAGTC CTTGCCAAGG CCATTGCTAC GGATACTGTA
    GCCAATTTGA GCAGTGGTCC AAAGCCTCCA TTTTTTGAAG AACTGATGTC TCCATTAATT
    CCAAATATTG TGGATAGAGC ACCTGAAGGC ACAACTTTTG GGGATGTCCT GCTACCAGCG
    AACACTATAT ACAGAGTGGG AGAAGTTGTT GAAGTTACCT TTGTAGGTGC TAACCCAAAG
    AATTCAGCAG AAAACCGGAC CCATCAGACC TTCCTCACTG TGGAGAAATA TGAGGCCACA
    TCAGCAACAT GGCAGATAAT GCATAATGAT GCCTCCTGGG AGACTCGTTT CTACTGGCAC
    AAAGGCTCAC TGGGTCTTAG CAATGCGACG ATACAGTGGC ACATTCCAGA TACTGCCCAG
    CCTGGAACCT ACAGAATAAA ATACTTTGGA CACAGTCGGA AGCAGGACTT ACTCAAGCCC
    GCTGTTCTAC TTTCATTTGA AAGCACTCCC TCTGTTTTTG AAGTCACAAC TACTTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015316081.1
    CDS201..2540
    Translation

    Target ORF information:

    RefSeq Version XM_015460595.1
    Organism Bos taurus(cattle)
    Definition Bos taurus N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2 (ASAH2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015460595.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCCAAAC GCACCTTTTC CAGCTTTGAA ATATTCCTGA TTTTCCTCCT TGTGATAACG 
    ACAGGGATCA CGGTGGCTCT TCTCAGCCTG TTGTTTATCA CCAGTGGGAC CATTGAAAAT
    CACACAGGCA AGTATCGTTT TCCATCCACC CAGGGCCCTC CAGCTACCCA GAGCCCTCCA
    GCTACCCAGG GCTCTGCAGC CCCTCAAAAC TCAACACCCT CCCGGGGCCC CACAACTACC
    CAGGCATCTC CAACACCCAG CACCCCAGAG CCTACCCTGT TTCAGAACTT CAGTGGCTAC
    CATATTGGTG TTGGGCGAGC TGACTGCACA GGACAAGTAT CAGATATTAA TTTGATGGGC
    TACGCCAAAA CTGGCCAGTA TGCACGGGGC ATCCTCACGA GGTTGTACAG TCGTGCGTTC
    ATCATGGCGG AGCCGGATGG GTCCAATCGC ATAGTGTTTG TCAGCATCGA CATAGGCATG
    GTATCCCAGC GACTCAGGCT GGAGGTCCTG AGCAGGCTGC AGAATAAATA CGGCTCCCTG
    TACAGAAGAG ACAATGTTAT TCTGAGTGGC ACTCATACAC ACTCGGGTCC AGCAGGATAT
    TTCCAGTACA CGACATTTGT AATTGCCAGT GAAGGATTTA GCAATCGAAC TTTTGAGTAC
    ATGGTCACTG GCATTGTGGA GAGCGTTGAG ATAGCACACA AAAATATGAA ACCAGGCAAA
    ATCTTTATCA ATAAAGGGAC TGTGGATGGT GCACAGATCA ACCGAAGTCC TACTTCTTAC
    CTTTGGAATC CACAGTCAGA GAGAGCAAGG TATTCTTCAA ATACAGACAA GGAAATGGTG
    CTCCTGAAAA TGGTAGATCT GAATGGAGAA GAGCTGGGTC TTATCAGCTG GTTTGCCATC
    CACCCAGTCA GCATGAAAAA CACCAATCAT CTTGTAAATA GTGACAACGT AGGTTATGCA
    TCTTACCTTT TTGAGCAAGA GAAGAACAAA GGTTATCTAC CTGGAGAGGG ACCGTATGTA
    GCAGCTTTTG CTTCATCAAA TCTGGGAGAT GTTTCCCCCA ATGTTCTTGG CCCGCAATGC
    ACCAACACGG GAGAGTCTTG TGATAACGCC AATAGCTCTT GTCCCATTGG TGGGCCTAGC
    ATGTGTGTGG CTATGGGACC TGGGCAGGAT ATGTTTGAAA GTACACAAAT CATAGGACGG
    ATAATATATG ACAAAGCAAA GGAACTGTAT GAGTCTGCCT CCCAGGAGGT AACTGGGCCA
    CTGGCTTCAG CTCATCAGTG GGTGAATATG ACGGATGTCA CCGTCTGGCT CAATTCAACA
    CACACAGTAA AAACGTGTAA ACCAGCCTTG GGCTACAGTT TTGCAGCTGG TACAATCGAT
    GGATTTGGAA CCGTCAGTTT TACTCAGGGG ATGACAGAAG GCGATTCATT TTGGGACACT
    CTTCGGGATC AGGTTCTGGG CAAACCATCT GAAGAAATCA AAGAATGCCA TAAACCAAAG
    CCAGTCCTTC TTCAGACTGG AGAGCTGTTA AATCCTCATC CCTGGCACCC AGATATCGTT
    GACGTTCAGA TTGTTACTCT CGGGTTCTTG GCCATCACTG CCATCCCTGG GGAGTTCACG
    ACCATGTCTG GACGGAGACT TCGGCAGGCA GTTCGAGAAG AATTTGAAGC TTATGGGATG
    CTGAATATGA CTGTTGTTAT TTCAGGTCTA TGTAATGTAT ATACACACTA CATTACCACA
    TATGAAGAAT ACCAGGCGCA GCGGTACGAG GCCGCATCCA CAATTTATGG ACCGCATACT
    CTGTCTGCCT ACATCCAGCT TTTCAGAGTC CTTGCCAAGG CCATTGCTAC GGATACTGTA
    GCCAATTTGA GCAGTGGTCC AAAGCCTCCA TTTTTTGAAG AACTGATGTC TCCATTAATT
    CCAAATATTG TGGATAGAGC ACCTGAAGGC ACAACTTTTG GGGATGTCCT GCTACCAGCG
    AACACTATAT ACAGAGTGGG AGAAGTTGTT GAAGTTACCT TTGTAGGTGC TAACCCAAAG
    AATTCAGCAG AAAACCGGAC CCATCAGACC TTCCTCACTG TGGAGAAATA TGAGGCCACA
    TCAGCAACAT GGCAGATAAT GCATAATGAT GCCTCCTGGG AGACTCGTTT CTACTGGCAC
    AAAGGCTCAC TGGGTCTTAG CAATGCGACG ATACAGTGGC ACATTCCAGA TACTGCCCAG
    CCTGGAACCT ACAGAATAAA ATACTTTGGA CACAGTCGGA AGCAGGACTT ACTCAAGCCC
    GCTGTTCTAC TTTCATTTGA AAGCACTCCC TCTGTTTTTG AAGTCACAAC TACTTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.