Asah2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Asah2
Entrez Gene ID	54447
Full Name	N-acylsphingosine amidohydrolase 2
Synonyms	AI585898
General protein information	Preferred Names N-acylsphingosine amidohydrolase 2 Names neutral ceramidase N-CDase NCDase acylsphingosine deacylase 2 neutral/alkaline ceramidase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 19 Map Location: 19\|19 C1

mRNA and Protein(s)

mRNA	Protein	Name
XM_011247289.1	XP_011245591.1	neutral ceramidase isoform X1
XM_011247284.2	XP_011245586.1	neutral ceramidase isoform X1
XM_011247285.2	XP_011245587.1	neutral ceramidase isoform X1
XM_011247283.2	XP_011245585.1	neutral ceramidase isoform X1
XM_011247286.2	XP_011245588.1	neutral ceramidase isoform X1
XM_011247287.2	XP_011245589.1	neutral ceramidase isoform X1
XM_011247290.2	XP_011245592.1	neutral ceramidase isoform X1
NM_018830.1	NP_061300.1	neutral ceramidase

Pathways from BioSystems

Homologies

Homo sapiens (human)	ASAH2	NP_063946.2
Drosophila melanogaster (fruit fly)	CDase	NP_733370.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000973	XP_309165.3
Arabidopsis thaliana (thale cress)	AT2G38010	NP_181337.2
Xenopus tropicalis (tropical clawed frog)	asah2	NP_001123830.1
Xenopus tropicalis (tropical clawed frog)	LOC100494440	XP_002935475.2
	ASAH2	XP_001100613.1
Bos taurus (cattle)	ASAH2	XP_002698412.1
Mus musculus (house mouse)	Asah2	NP_061300.1
Rattus norvegicus (Norway rat)	Asah2	NP_446098.1
Danio rerio (zebrafish)	asah2	NP_001007764.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003730	XP_310261.7
Gallus gallus (chicken)	ASAH2	XP_421560.4
Oryza sativa (rice)	Os01g0624000	NP_001043618.1
Pan troglodytes (chimpanzee)	ASAH2	XP_507791.2
Canis lupus familiaris (dog)	ASAH2B	XP_005636694.1

Gene Ontology
Bibliography

Related articles in PubMed

Lactosylceramide contributes to mitochondrial dysfunction in diabetes.
Novgorodov SA, Riley CL, Yu J, Keffler JA, Clarke CJ, Van Laer AO, Baicu CF, Zile MR, Gudz TI
Journal of lipid research57(4)546-62(2016 Apr)

Loss of neutral ceramidase protects cells from nutrient- and energy -deprivation-induced cell death.
Sundaram K, Mather AR, Marimuthu S, Shah PP, Snider AJ, Obeid LM, Hannun YA, Beverly LJ, Siskind LJ
The Biochemical journal473(6)743-55(2016 Mar)

Essential roles of neutral ceramidase and sphingosine in mitochondrial dysfunction due to traumatic brain injury.
Novgorodov SA, Riley CL, Yu J, Borg KT, Hannun YA, Proia RL, Kindy MS, Gudz TI
The Journal of biological chemistry289(19)13142-54(2014 May)

Transcription factor Ctip2 controls epidermal lipid metabolism and regulates expression of genes involved in sphingolipid biosynthesis during skin development.
Wang Z, Kirkwood JS, Taylor AW, Stevens JF, Leid M, Ganguli-Indra G, Indra AK
The Journal of investigative dermatology133(3)668-676(2013 Mar)

Loss of neutral ceramidase increases inflammation in a mouse model of inflammatory bowel disease.
Snider AJ, Wu BX, Jenkins RW, Sticca JA, Kawamori T, Hannun YA, Obeid LM
Prostaglandins & other lipid mediators99(3-4)124-30(2012 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results suggest that neutral ceramidase is a mediator of necroptosis and might be a novel therapeutic target for protection from ischaemic injury.
Title: Loss of neutral ceramidase protects cells from nutrient- and energy -deprivation-induced cell death.

Knocking down NCDase reduced sphingosine accumulation in mitochondria and preserved COX activity after the brain injury.
Title: Essential roles of neutral ceramidase and sphingosine in mitochondrial dysfunction due to traumatic brain injury.

Neutral ceramidase deficiency increases inflammation in a mouse model of inflammatory bowel disease.
Title: Loss of neutral ceramidase increases inflammation in a mouse model of inflammatory bowel disease.

Novel pathway of ceramide production in mitochondria: thioesterase and neutral ceramidase produce ceramide from sphingosine and acyl-CoA.
Title: Novel pathway of ceramide production in mitochondria: thioesterase and neutral ceramidase produce ceramide from sphingosine and acyl-CoA.

the predicted HNF-4alpha binding site identified in the Asah2 promoter is functional
Title: Identification of a functional hepatocyte nuclear factor 4 binding site in the neutral ceramidase promoter.

The following Asah2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Asah2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu10953	NM_018830.1 Latest version!	Mus musculus N-acylsphingosine amidohydrolase 2 (Asah2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu10953	XM_011247284.2 Latest version!	Mus musculus N-acylsphingosine amidohydrolase 2 (Asah2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu10953	XM_011247289.1 Latest version!	Mus musculus N-acylsphingosine amidohydrolase 2 (Asah2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu10953	XM_011247283.2 Latest version!	Mus musculus N-acylsphingosine amidohydrolase 2 (Asah2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu10953	XM_011247290.2 Latest version!	Mus musculus N-acylsphingosine amidohydrolase 2 (Asah2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu10953	XM_011247285.2 Latest version!	Mus musculus N-acylsphingosine amidohydrolase 2 (Asah2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu10953	XM_011247286.2 Latest version!	Mus musculus N-acylsphingosine amidohydrolase 2 (Asah2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu10953	XM_011247287.2 Latest version!	Mus musculus N-acylsphingosine amidohydrolase 2 (Asah2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Asah2 ( NM_018830.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_061300.1 Mus musculus N-acylsphingosine amidohydrolase 2 (Asah2), mRNA.

Price & Availability↑

CloneID	OMu10953
Clone ID Related Accession (Same CDS sequence)	XM_011247289.1 , XM_011247284.2 , XM_011247285.2 , XM_011247283.2 , XM_011247286.2 , XM_011247287.2 , XM_011247290.2 , NM_018830.1
Accession Version	NM_018830.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2271bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Mus musculus(house mouse)
Product	neutral ceramidase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB037181.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB037181.1, BC137898.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGCAAAGC GAACCTTCTC CACCTTGGAG GCATTCCTCA TTTTCCTTCT GGTAATAATG 
ACAGTCATCA CAGTGGCCCT TCTCACCCTC TTGTTTGTTA CCAGTGGGAC CATTGAAAAC 
CACAAAGATT CAGGAAATCA CTGGTTTTCA ACCACTCTGG GCTCCACGAC AACCCAGCCC 
CCTCCAATTA CACAGACTCC AAACTTCCCT TCATTTCGGA ACTTCAGTGG CTACTACATT 
GGCGTTGGGA GAGCAGATTG CACAGGACAA GTGTCAGATA TCAATTTGAT GGGCTATGGC 
AAAAATGGCC AGAATGCACG GGGTCTCCTC ACCAGGCTGT TCAGCCGTGC TTTTATCTTG 
GCGGATCCAG ATGGGTCAAA TCGAATGGCA TTTGTGAGCG TGGAACTATG TATGATTTCC 
CAACGACTGA GGTTGGAGGT CCTGAAGAGA CTAGAGAGTA AATATGGCTC TCTGTATCGA 
AGAGACAATG TTATCCTGAG TGCCATTCAC ACACACTCTG GCCCAGCAGG GTTTTTCCAA 
TATACACTCT ATATACTCGC CAGCGAGGGA TTCAGCAACC GGACCTTTCA GTACATAGTC 
TCTGGGATCA TGAAGAGCAT TGATATAGCT CACACAAATC TTAAACCAGG CAAAATCTTT 
ATCAACAAAG GAAATGTTGC TAATGTGCAG ATCAACCGAA GCCCCTCCTC TTACCTTCTG 
AATCCACAGT CAGAGAGAGC AAGGTATTCT TCAAACACAG ACAAGGAAAT GCTGGTCTTG 
AAACTGGTGG ATTTGAATGG AGAAGACTTG GGTCTTATCA GCTGGTTTGC CATCCACCCC 
GTGAGCATGA ACAATAGCAA CCACTTTGTG AATAGTGACA ATATGGGCTA TGCGGCTTAC 
CTTTTTGAGC AAGAAAAGAA CAAAGGCTAT CTGCCTGGAC AGGGACCGTT TGTAGCAGGC 
TTTGCTTCAT CAAATCTCGG AGACGTGTCA CCCAACATTC TTGGCCCGCA TTGTGTCAAC 
ACAGGGGAGT CTTGTGACAA CGACAAGAGC ACCTGTCCCA ACGGTGGGCC TAGCATGTGC 
ATGGCCAGCG GACCTGGACA AGACATGTTT GAGAGCACAC ACATTATAGG ACGGATCATC 
TATCAGAAGG CCAAGGAGCT GTATGCCTCT GCCTCCCAGG AGGTGACCGG CCCAGTGCTT 
GCAGCTCACC AGTGGGTGAA CATGACAGAT GTGAGCGTCC AGCTCAATGC CACACACACA 
GTGAAGACGT GTAAACCTGC CCTGGGCTAC AGTTTTGCCG CAGGCACAAT TGATGGAGTT 
TCGGGCCTCA ATATTACACA GGGAACTACG GAAGGGGATC CATTCTGGGA CACTCTTCGG 
GACCAGCTCT TGGGAAAACC ATCTGAAGAG ATTGTAGAGT GTCAGAAACC CAAACCAATC 
CTGCTTCACA GTGGAGAGCT GACGATACCA CATCCTTGGC AACCAGATAT TGTTGATGTT 
CAGATTGTTA CCGTTGGGTC CTTGGCCATA GCTGCTATCC CTGGGGAATT AACAACCATG 
TCGGGACGAA GATTTCGTGA GGCAATTAAA AAAGAATTTG CACTTTATGG GATGAAGGAT 
ATGACCGTTG TTATCGCAGG TCTAAGCAAT GTTTATACAC ATTACATTAC CACATATGAA 
GAATACCAGG CTCAGCGGTA CGAGGCAGCA TCTACAATCT ATGGACCACA CACCCTGTCT 
GCATACATCC AACTCTTCAG AGACCTTGCT AAGGCAATTG CTACGGACAC AGTAGCCAAC 
ATGAGCAGTG GTCCCGAGCC TCCATTCTTC AAAAATCTAA TAGCTTCACT TATTCCTAAT 
ATTGCGGATA GAGCACCAAT TGGCAAACAT TTTGGGGATG TCTTGCAGCC AGCAAAACCT 
GAATACAGAG TGGGAGAAGT GGTTGAAGTT ATATTTGTAG GCGCTAACCC AAAGAATTCA 
GCAGAGAACC AGACCCATCA AACCTTCCTC ACTGTGGAGA AATACGAGGA CTCTGTAGCT 
GACTGGCAGA TAATGTATAA CGATGCCTCC TGGGAGACGA GGTTTTATTG GCACAAAGGA 
ATACTGGGTC TGAGCAATGC AACAATATAC TGGCATATTC CAGATACTGC CTACCCTGGA 
ATCTACAGAA TAAGATATTT TGGACACAAT CGGAAGCAGG AACTTCTGAA ACCCGCTGTC 
ATACTAGCAT TTGAAGGAAT TTCTTCTCCT TTTGAAGTTG TCACTACTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_061300.1
CDS	441..2711
Translation	MAKRTFSTLEAFLIFLLVIMTVITVALLTLLFVTSGTIENHKDSGNHWFSTTLGSTTTQPPPITQTPNFPSFRNFSGYYIGVGRADCTGQVSDINLMGYGKNGQNARGLLTRLFSRAFILADPDGSNRMAFVSVELCMISQRLRLEVLKRLESKYGSLYRRDNVILSAIHTHSGPAGFFQYTLYILASEGFSNRTFQYIVSGIMKSIDIAHTNLKPGKIFINKGNVANVQINRSPSSYLLNPQSERARYSSNTDKEMLVLKLVDLNGEDLGLISWFAIHPVSMNNSNHFVNSDNMGYAAYLFEQEKNKGYLPGQGPFVAGFASSNLGDVSPNILGPHCVNTGESCDNDKSTCPNGGPSMCMASGPGQDMFESTHIIGRIIYQKAKELYASASQEVTGPVLAAHQWVNMTDVSVQLNATHTVKTCKPALGYSFAAGTIDGVSGLNITQGTTEGDPFWDTLRDQLLGKPSEEIVECQKPKPILLHSGELTIPHPWQPDIVDVQIVTVGSLAIAAIPGELTTMSGRRFREAIKKEFALYGMKDMTVVIAGLSNVYTHYITTYEEYQAQRYEAASTIYGPHTLSAYIQLFRDLAKAIATDTVANMSSGPEPPFFKNLIASLIPNIADRAPIGKHFGDVLQPAKPEYRVGEVVEVIFVGANPKNSAENQTHQTFLTVEKYEDSVADWQIMYNDASWETRFYWHKGILGLSNATIYWHIPDTAYPGIYRIRYFGHNRKQELLKPAVILAFEGISSPFEVVTT

Target ORF information:

RefSeq Version	NM_018830.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus N-acylsphingosine amidohydrolase 2 (Asah2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018830.1

ORF Insert Sequence:

ATGGCAAAGC GAACCTTCTC CACCTTGGAG GCATTCCTCA TTTTCCTTCT GGTAATAATG 
ACAGTCATCA CAGTGGCCCT TCTCACCCTC TTGTTTGTTA CCAGTGGGAC CATTGAAAAC 
CACAAAGATT CAGGAAATCA CTGGTTTTCA ACCACTCTGG GCTCCACGAC AACCCAGCCC 
CCTCCAATTA CACAGACTCC AAACTTCCCT TCATTTCGGA ACTTCAGTGG CTACTACATT 
GGCGTTGGGA GAGCAGATTG CACAGGACAA GTGTCAGATA TCAATTTGAT GGGCTATGGC 
AAAAATGGCC AGAATGCACG GGGTCTCCTC ACCAGGCTGT TCAGCCGTGC TTTTATCTTG 
GCGGATCCAG ATGGGTCAAA TCGAATGGCA TTTGTGAGCG TGGAACTATG TATGATTTCC 
CAACGACTGA GGTTGGAGGT CCTGAAGAGA CTAGAGAGTA AATATGGCTC TCTGTATCGA 
AGAGACAATG TTATCCTGAG TGCCATTCAC ACACACTCTG GCCCAGCAGG GTTTTTCCAA 
TATACACTCT ATATACTCGC CAGCGAGGGA TTCAGCAACC GGACCTTTCA GTACATAGTC 
TCTGGGATCA TGAAGAGCAT TGATATAGCT CACACAAATC TTAAACCAGG CAAAATCTTT 
ATCAACAAAG GAAATGTTGC TAATGTGCAG ATCAACCGAA GCCCCTCCTC TTACCTTCTG 
AATCCACAGT CAGAGAGAGC AAGGTATTCT TCAAACACAG ACAAGGAAAT GCTGGTCTTG 
AAACTGGTGG ATTTGAATGG AGAAGACTTG GGTCTTATCA GCTGGTTTGC CATCCACCCC 
GTGAGCATGA ACAATAGCAA CCACTTTGTG AATAGTGACA ATATGGGCTA TGCGGCTTAC 
CTTTTTGAGC AAGAAAAGAA CAAAGGCTAT CTGCCTGGAC AGGGACCGTT TGTAGCAGGC 
TTTGCTTCAT CAAATCTCGG AGACGTGTCA CCCAACATTC TTGGCCCGCA TTGTGTCAAC 
ACAGGGGAGT CTTGTGACAA CGACAAGAGC ACCTGTCCCA ACGGTGGGCC TAGCATGTGC 
ATGGCCAGCG GACCTGGACA AGACATGTTT GAGAGCACAC ACATTATAGG ACGGATCATC 
TATCAGAAGG CCAAGGAGCT GTATGCCTCT GCCTCCCAGG AGGTGACCGG CCCAGTGCTT 
GCAGCTCACC AGTGGGTGAA CATGACAGAT GTGAGCGTCC AGCTCAATGC CACACACACA 
GTGAAGACGT GTAAACCTGC CCTGGGCTAC AGTTTTGCCG CAGGCACAAT TGATGGAGTT 
TCGGGCCTCA ATATTACACA GGGAACTACG GAAGGGGATC CATTCTGGGA CACTCTTCGG 
GACCAGCTCT TGGGAAAACC ATCTGAAGAG ATTGTAGAGT GTCAGAAACC CAAACCAATC 
CTGCTTCACA GTGGAGAGCT GACGATACCA CATCCTTGGC AACCAGATAT TGTTGATGTT 
CAGATTGTTA CCGTTGGGTC CTTGGCCATA GCTGCTATCC CTGGGGAATT AACAACCATG 
TCGGGACGAA GATTTCGTGA GGCAATTAAA AAAGAATTTG CACTTTATGG GATGAAGGAT 
ATGACCGTTG TTATCGCAGG TCTAAGCAAT GTTTATACAC ATTACATTAC CACATATGAA 
GAATACCAGG CTCAGCGGTA CGAGGCAGCA TCTACAATCT ATGGACCACA CACCCTGTCT 
GCATACATCC AACTCTTCAG AGACCTTGCT AAGGCAATTG CTACGGACAC AGTAGCCAAC 
ATGAGCAGTG GTCCCGAGCC TCCATTCTTC AAAAATCTAA TAGCTTCACT TATTCCTAAT 
ATTGCGGATA GAGCACCAAT TGGCAAACAT TTTGGGGATG TCTTGCAGCC AGCAAAACCT 
GAATACAGAG TGGGAGAAGT GGTTGAAGTT ATATTTGTAG GCGCTAACCC AAAGAATTCA 
GCAGAGAACC AGACCCATCA AACCTTCCTC ACTGTGGAGA AATACGAGGA CTCTGTAGCT 
GACTGGCAGA TAATGTATAA CGATGCCTCC TGGGAGACGA GGTTTTATTG GCACAAAGGA 
ATACTGGGTC TGAGCAATGC AACAATATAC TGGCATATTC CAGATACTGC CTACCCTGGA 
ATCTACAGAA TAAGATATTT TGGACACAAT CGGAAGCAGG AACTTCTGAA ACCCGCTGTC 
ATACTAGCAT TTGAAGGAAT TTCTTCTCCT TTTGAAGTTG TCACTACTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Asah2 ( XM_011247289.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011245591.1 Mus musculus N-acylsphingosine amidohydrolase 2 (Asah2), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OMu10953
Clone ID Related Accession (Same CDS sequence)	XM_011247289.1 , XM_011247284.2 , XM_011247285.2 , XM_011247283.2 , XM_011247286.2 , XM_011247287.2 , XM_011247290.2 , NM_018830.1
Accession Version	XM_011247289.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2271bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	neutral ceramidase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAAGC GAACCTTCTC CACCTTGGAG GCATTCCTCA TTTTCCTTCT GGTAATAATG 
ACAGTCATCA CAGTGGCCCT TCTCACCCTC TTGTTTGTTA CCAGTGGGAC CATTGAAAAC 
CACAAAGATT CAGGAAATCA CTGGTTTTCA ACCACTCTGG GCTCCACGAC AACCCAGCCC 
CCTCCAATTA CACAGACTCC AAACTTCCCT TCATTTCGGA ACTTCAGTGG CTACTACATT 
GGCGTTGGGA GAGCAGATTG CACAGGACAA GTGTCAGATA TCAATTTGAT GGGCTATGGC 
AAAAATGGCC AGAATGCACG GGGTCTCCTC ACCAGGCTGT TCAGCCGTGC TTTTATCTTG 
GCGGATCCAG ATGGGTCAAA TCGAATGGCA TTTGTGAGCG TGGAACTATG TATGATTTCC 
CAACGACTGA GGTTGGAGGT CCTGAAGAGA CTAGAGAGTA AATATGGCTC TCTGTATCGA 
AGAGACAATG TTATCCTGAG TGCCATTCAC ACACACTCTG GCCCAGCAGG GTTTTTCCAA 
TATACACTCT ATATACTCGC CAGCGAGGGA TTCAGCAACC GGACCTTTCA GTACATAGTC 
TCTGGGATCA TGAAGAGCAT TGATATAGCT CACACAAATC TTAAACCAGG CAAAATCTTT 
ATCAACAAAG GAAATGTTGC TAATGTGCAG ATCAACCGAA GCCCCTCCTC TTACCTTCTG 
AATCCACAGT CAGAGAGAGC AAGGTATTCT TCAAACACAG ACAAGGAAAT GCTGGTCTTG 
AAACTGGTGG ATTTGAATGG AGAAGACTTG GGTCTTATCA GCTGGTTTGC CATCCACCCC 
GTGAGCATGA ACAATAGCAA CCACTTTGTG AATAGTGACA ATATGGGCTA TGCGGCTTAC 
CTTTTTGAGC AAGAAAAGAA CAAAGGCTAT CTGCCTGGAC AGGGACCGTT TGTAGCAGGC 
TTTGCTTCAT CAAATCTCGG AGACGTGTCA CCCAACATTC TTGGCCCGCA TTGTGTCAAC 
ACAGGGGAGT CTTGTGACAA CGACAAGAGC ACCTGTCCCA ACGGTGGGCC TAGCATGTGC 
ATGGCCAGCG GACCTGGACA AGACATGTTT GAGAGCACAC ACATTATAGG ACGGATCATC 
TATCAGAAGG CCAAGGAGCT GTATGCCTCT GCCTCCCAGG AGGTGACCGG CCCAGTGCTT 
GCAGCTCACC AGTGGGTGAA CATGACAGAT GTGAGCGTCC AGCTCAATGC CACACACACA 
GTGAAGACGT GTAAACCTGC CCTGGGCTAC AGTTTTGCCG CAGGCACAAT TGATGGAGTT 
TCGGGCCTCA ATATTACACA GGGAACTACG GAAGGGGATC CATTCTGGGA CACTCTTCGG 
GACCAGCTCT TGGGAAAACC ATCTGAAGAG ATTGTAGAGT GTCAGAAACC CAAACCAATC 
CTGCTTCACA GTGGAGAGCT GACGATACCA CATCCTTGGC AACCAGATAT TGTTGATGTT 
CAGATTGTTA CCGTTGGGTC CTTGGCCATA GCTGCTATCC CTGGGGAATT AACAACCATG 
TCGGGACGAA GATTTCGTGA GGCAATTAAA AAAGAATTTG CACTTTATGG GATGAAGGAT 
ATGACCGTTG TTATCGCAGG TCTAAGCAAT GTTTATACAC ATTACATTAC CACATATGAA 
GAATACCAGG CTCAGCGGTA CGAGGCAGCA TCTACAATCT ATGGACCACA CACCCTGTCT 
GCATACATCC AACTCTTCAG AGACCTTGCT AAGGCAATTG CTACGGACAC AGTAGCCAAC 
ATGAGCAGTG GTCCCGAGCC TCCATTCTTC AAAAATCTAA TAGCTTCACT TATTCCTAAT 
ATTGCGGATA GAGCACCAAT TGGCAAACAT TTTGGGGATG TCTTGCAGCC AGCAAAACCT 
GAATACAGAG TGGGAGAAGT GGTTGAAGTT ATATTTGTAG GCGCTAACCC AAAGAATTCA 
GCAGAGAACC AGACCCATCA AACCTTCCTC ACTGTGGAGA AATACGAGGA CTCTGTAGCT 
GACTGGCAGA TAATGTATAA CGATGCCTCC TGGGAGACGA GGTTTTATTG GCACAAAGGA 
ATACTGGGTC TGAGCAATGC AACAATATAC TGGCATATTC CAGATACTGC CTACCCTGGA 
ATCTACAGAA TAAGATATTT TGGACACAAT CGGAAGCAGG AACTTCTGAA ACCCGCTGTC 
ATACTAGCAT TTGAAGGAAT TTCTTCTCCT TTTGAAGTTG TCACTACTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011245591.1
CDS	477..2747
Translation	MAKRTFSTLEAFLIFLLVIMTVITVALLTLLFVTSGTIENHKDSGNHWFSTTLGSTTTQPPPITQTPNFPSFRNFSGYYIGVGRADCTGQVSDINLMGYGKNGQNARGLLTRLFSRAFILADPDGSNRMAFVSVELCMISQRLRLEVLKRLESKYGSLYRRDNVILSAIHTHSGPAGFFQYTLYILASEGFSNRTFQYIVSGIMKSIDIAHTNLKPGKIFINKGNVANVQINRSPSSYLLNPQSERARYSSNTDKEMLVLKLVDLNGEDLGLISWFAIHPVSMNNSNHFVNSDNMGYAAYLFEQEKNKGYLPGQGPFVAGFASSNLGDVSPNILGPHCVNTGESCDNDKSTCPNGGPSMCMASGPGQDMFESTHIIGRIIYQKAKELYASASQEVTGPVLAAHQWVNMTDVSVQLNATHTVKTCKPALGYSFAAGTIDGVSGLNITQGTTEGDPFWDTLRDQLLGKPSEEIVECQKPKPILLHSGELTIPHPWQPDIVDVQIVTVGSLAIAAIPGELTTMSGRRFREAIKKEFALYGMKDMTVVIAGLSNVYTHYITTYEEYQAQRYEAASTIYGPHTLSAYIQLFRDLAKAIATDTVANMSSGPEPPFFKNLIASLIPNIADRAPIGKHFGDVLQPAKPEYRVGEVVEVIFVGANPKNSAENQTHQTFLTVEKYEDSVADWQIMYNDASWETRFYWHKGILGLSNATIYWHIPDTAYPGIYRIRYFGHNRKQELLKPAVILAFEGISSPFEVVTT

Target ORF information:

RefSeq Version	XM_011247289.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus N-acylsphingosine amidohydrolase 2 (Asah2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011247289.1

ORF Insert Sequence:

ATGGCAAAGC GAACCTTCTC CACCTTGGAG GCATTCCTCA TTTTCCTTCT GGTAATAATG 
ACAGTCATCA CAGTGGCCCT TCTCACCCTC TTGTTTGTTA CCAGTGGGAC CATTGAAAAC 
CACAAAGATT CAGGAAATCA CTGGTTTTCA ACCACTCTGG GCTCCACGAC AACCCAGCCC 
CCTCCAATTA CACAGACTCC AAACTTCCCT TCATTTCGGA ACTTCAGTGG CTACTACATT 
GGCGTTGGGA GAGCAGATTG CACAGGACAA GTGTCAGATA TCAATTTGAT GGGCTATGGC 
AAAAATGGCC AGAATGCACG GGGTCTCCTC ACCAGGCTGT TCAGCCGTGC TTTTATCTTG 
GCGGATCCAG ATGGGTCAAA TCGAATGGCA TTTGTGAGCG TGGAACTATG TATGATTTCC 
CAACGACTGA GGTTGGAGGT CCTGAAGAGA CTAGAGAGTA AATATGGCTC TCTGTATCGA 
AGAGACAATG TTATCCTGAG TGCCATTCAC ACACACTCTG GCCCAGCAGG GTTTTTCCAA 
TATACACTCT ATATACTCGC CAGCGAGGGA TTCAGCAACC GGACCTTTCA GTACATAGTC 
TCTGGGATCA TGAAGAGCAT TGATATAGCT CACACAAATC TTAAACCAGG CAAAATCTTT 
ATCAACAAAG GAAATGTTGC TAATGTGCAG ATCAACCGAA GCCCCTCCTC TTACCTTCTG 
AATCCACAGT CAGAGAGAGC AAGGTATTCT TCAAACACAG ACAAGGAAAT GCTGGTCTTG 
AAACTGGTGG ATTTGAATGG AGAAGACTTG GGTCTTATCA GCTGGTTTGC CATCCACCCC 
GTGAGCATGA ACAATAGCAA CCACTTTGTG AATAGTGACA ATATGGGCTA TGCGGCTTAC 
CTTTTTGAGC AAGAAAAGAA CAAAGGCTAT CTGCCTGGAC AGGGACCGTT TGTAGCAGGC 
TTTGCTTCAT CAAATCTCGG AGACGTGTCA CCCAACATTC TTGGCCCGCA TTGTGTCAAC 
ACAGGGGAGT CTTGTGACAA CGACAAGAGC ACCTGTCCCA ACGGTGGGCC TAGCATGTGC 
ATGGCCAGCG GACCTGGACA AGACATGTTT GAGAGCACAC ACATTATAGG ACGGATCATC 
TATCAGAAGG CCAAGGAGCT GTATGCCTCT GCCTCCCAGG AGGTGACCGG CCCAGTGCTT 
GCAGCTCACC AGTGGGTGAA CATGACAGAT GTGAGCGTCC AGCTCAATGC CACACACACA 
GTGAAGACGT GTAAACCTGC CCTGGGCTAC AGTTTTGCCG CAGGCACAAT TGATGGAGTT 
TCGGGCCTCA ATATTACACA GGGAACTACG GAAGGGGATC CATTCTGGGA CACTCTTCGG 
GACCAGCTCT TGGGAAAACC ATCTGAAGAG ATTGTAGAGT GTCAGAAACC CAAACCAATC 
CTGCTTCACA GTGGAGAGCT GACGATACCA CATCCTTGGC AACCAGATAT TGTTGATGTT 
CAGATTGTTA CCGTTGGGTC CTTGGCCATA GCTGCTATCC CTGGGGAATT AACAACCATG 
TCGGGACGAA GATTTCGTGA GGCAATTAAA AAAGAATTTG CACTTTATGG GATGAAGGAT 
ATGACCGTTG TTATCGCAGG TCTAAGCAAT GTTTATACAC ATTACATTAC CACATATGAA 
GAATACCAGG CTCAGCGGTA CGAGGCAGCA TCTACAATCT ATGGACCACA CACCCTGTCT 
GCATACATCC AACTCTTCAG AGACCTTGCT AAGGCAATTG CTACGGACAC AGTAGCCAAC 
ATGAGCAGTG GTCCCGAGCC TCCATTCTTC AAAAATCTAA TAGCTTCACT TATTCCTAAT 
ATTGCGGATA GAGCACCAAT TGGCAAACAT TTTGGGGATG TCTTGCAGCC AGCAAAACCT 
GAATACAGAG TGGGAGAAGT GGTTGAAGTT ATATTTGTAG GCGCTAACCC AAAGAATTCA 
GCAGAGAACC AGACCCATCA AACCTTCCTC ACTGTGGAGA AATACGAGGA CTCTGTAGCT 
GACTGGCAGA TAATGTATAA CGATGCCTCC TGGGAGACGA GGTTTTATTG GCACAAAGGA 
ATACTGGGTC TGAGCAATGC AACAATATAC TGGCATATTC CAGATACTGC CTACCCTGGA 
ATCTACAGAA TAAGATATTT TGGACACAAT CGGAAGCAGG AACTTCTGAA ACCCGCTGTC 
ATACTAGCAT TTGAAGGAAT TTCTTCTCCT TTTGAAGTTG TCACTACTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Asah2 ( XM_011247284.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011245586.1 Mus musculus N-acylsphingosine amidohydrolase 2 (Asah2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu10953
Clone ID Related Accession (Same CDS sequence)	XM_011247289.1 , XM_011247284.2 , XM_011247285.2 , XM_011247283.2 , XM_011247286.2 , XM_011247287.2 , XM_011247290.2 , NM_018830.1
Accession Version	XM_011247284.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2271bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	neutral ceramidase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011247284.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAAGC GAACCTTCTC CACCTTGGAG GCATTCCTCA TTTTCCTTCT GGTAATAATG 
ACAGTCATCA CAGTGGCCCT TCTCACCCTC TTGTTTGTTA CCAGTGGGAC CATTGAAAAC 
CACAAAGATT CAGGAAATCA CTGGTTTTCA ACCACTCTGG GCTCCACGAC AACCCAGCCC 
CCTCCAATTA CACAGACTCC AAACTTCCCT TCATTTCGGA ACTTCAGTGG CTACTACATT 
GGCGTTGGGA GAGCAGATTG CACAGGACAA GTGTCAGATA TCAATTTGAT GGGCTATGGC 
AAAAATGGCC AGAATGCACG GGGTCTCCTC ACCAGGCTGT TCAGCCGTGC TTTTATCTTG 
GCGGATCCAG ATGGGTCAAA TCGAATGGCA TTTGTGAGCG TGGAACTATG TATGATTTCC 
CAACGACTGA GGTTGGAGGT CCTGAAGAGA CTAGAGAGTA AATATGGCTC TCTGTATCGA 
AGAGACAATG TTATCCTGAG TGCCATTCAC ACACACTCTG GCCCAGCAGG GTTTTTCCAA 
TATACACTCT ATATACTCGC CAGCGAGGGA TTCAGCAACC GGACCTTTCA GTACATAGTC 
TCTGGGATCA TGAAGAGCAT TGATATAGCT CACACAAATC TTAAACCAGG CAAAATCTTT 
ATCAACAAAG GAAATGTTGC TAATGTGCAG ATCAACCGAA GCCCCTCCTC TTACCTTCTG 
AATCCACAGT CAGAGAGAGC AAGGTATTCT TCAAACACAG ACAAGGAAAT GCTGGTCTTG 
AAACTGGTGG ATTTGAATGG AGAAGACTTG GGTCTTATCA GCTGGTTTGC CATCCACCCC 
GTGAGCATGA ACAATAGCAA CCACTTTGTG AATAGTGACA ATATGGGCTA TGCGGCTTAC 
CTTTTTGAGC AAGAAAAGAA CAAAGGCTAT CTGCCTGGAC AGGGACCGTT TGTAGCAGGC 
TTTGCTTCAT CAAATCTCGG AGACGTGTCA CCCAACATTC TTGGCCCGCA TTGTGTCAAC 
ACAGGGGAGT CTTGTGACAA CGACAAGAGC ACCTGTCCCA ACGGTGGGCC TAGCATGTGC 
ATGGCCAGCG GACCTGGACA AGACATGTTT GAGAGCACAC ACATTATAGG ACGGATCATC 
TATCAGAAGG CCAAGGAGCT GTATGCCTCT GCCTCCCAGG AGGTGACCGG CCCAGTGCTT 
GCAGCTCACC AGTGGGTGAA CATGACAGAT GTGAGCGTCC AGCTCAATGC CACACACACA 
GTGAAGACGT GTAAACCTGC CCTGGGCTAC AGTTTTGCCG CAGGCACAAT TGATGGAGTT 
TCGGGCCTCA ATATTACACA GGGAACTACG GAAGGGGATC CATTCTGGGA CACTCTTCGG 
GACCAGCTCT TGGGAAAACC ATCTGAAGAG ATTGTAGAGT GTCAGAAACC CAAACCAATC 
CTGCTTCACA GTGGAGAGCT GACGATACCA CATCCTTGGC AACCAGATAT TGTTGATGTT 
CAGATTGTTA CCGTTGGGTC CTTGGCCATA GCTGCTATCC CTGGGGAATT AACAACCATG 
TCGGGACGAA GATTTCGTGA GGCAATTAAA AAAGAATTTG CACTTTATGG GATGAAGGAT 
ATGACCGTTG TTATCGCAGG TCTAAGCAAT GTTTATACAC ATTACATTAC CACATATGAA 
GAATACCAGG CTCAGCGGTA CGAGGCAGCA TCTACAATCT ATGGACCACA CACCCTGTCT 
GCATACATCC AACTCTTCAG AGACCTTGCT AAGGCAATTG CTACGGACAC AGTAGCCAAC 
ATGAGCAGTG GTCCCGAGCC TCCATTCTTC AAAAATCTAA TAGCTTCACT TATTCCTAAT 
ATTGCGGATA GAGCACCAAT TGGCAAACAT TTTGGGGATG TCTTGCAGCC AGCAAAACCT 
GAATACAGAG TGGGAGAAGT GGTTGAAGTT ATATTTGTAG GCGCTAACCC AAAGAATTCA 
GCAGAGAACC AGACCCATCA AACCTTCCTC ACTGTGGAGA AATACGAGGA CTCTGTAGCT 
GACTGGCAGA TAATGTATAA CGATGCCTCC TGGGAGACGA GGTTTTATTG GCACAAAGGA 
ATACTGGGTC TGAGCAATGC AACAATATAC TGGCATATTC CAGATACTGC CTACCCTGGA 
ATCTACAGAA TAAGATATTT TGGACACAAT CGGAAGCAGG AACTTCTGAA ACCCGCTGTC 
ATACTAGCAT TTGAAGGAAT TTCTTCTCCT TTTGAAGTTG TCACTACTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011245586.1
CDS	707..2977
Translation	MAKRTFSTLEAFLIFLLVIMTVITVALLTLLFVTSGTIENHKDSGNHWFSTTLGSTTTQPPPITQTPNFPSFRNFSGYYIGVGRADCTGQVSDINLMGYGKNGQNARGLLTRLFSRAFILADPDGSNRMAFVSVELCMISQRLRLEVLKRLESKYGSLYRRDNVILSAIHTHSGPAGFFQYTLYILASEGFSNRTFQYIVSGIMKSIDIAHTNLKPGKIFINKGNVANVQINRSPSSYLLNPQSERARYSSNTDKEMLVLKLVDLNGEDLGLISWFAIHPVSMNNSNHFVNSDNMGYAAYLFEQEKNKGYLPGQGPFVAGFASSNLGDVSPNILGPHCVNTGESCDNDKSTCPNGGPSMCMASGPGQDMFESTHIIGRIIYQKAKELYASASQEVTGPVLAAHQWVNMTDVSVQLNATHTVKTCKPALGYSFAAGTIDGVSGLNITQGTTEGDPFWDTLRDQLLGKPSEEIVECQKPKPILLHSGELTIPHPWQPDIVDVQIVTVGSLAIAAIPGELTTMSGRRFREAIKKEFALYGMKDMTVVIAGLSNVYTHYITTYEEYQAQRYEAASTIYGPHTLSAYIQLFRDLAKAIATDTVANMSSGPEPPFFKNLIASLIPNIADRAPIGKHFGDVLQPAKPEYRVGEVVEVIFVGANPKNSAENQTHQTFLTVEKYEDSVADWQIMYNDASWETRFYWHKGILGLSNATIYWHIPDTAYPGIYRIRYFGHNRKQELLKPAVILAFEGISSPFEVVTT

Target ORF information:

RefSeq Version	XM_011247284.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus N-acylsphingosine amidohydrolase 2 (Asah2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011247284.2

ORF Insert Sequence:

ATGGCAAAGC GAACCTTCTC CACCTTGGAG GCATTCCTCA TTTTCCTTCT GGTAATAATG 
ACAGTCATCA CAGTGGCCCT TCTCACCCTC TTGTTTGTTA CCAGTGGGAC CATTGAAAAC 
CACAAAGATT CAGGAAATCA CTGGTTTTCA ACCACTCTGG GCTCCACGAC AACCCAGCCC 
CCTCCAATTA CACAGACTCC AAACTTCCCT TCATTTCGGA ACTTCAGTGG CTACTACATT 
GGCGTTGGGA GAGCAGATTG CACAGGACAA GTGTCAGATA TCAATTTGAT GGGCTATGGC 
AAAAATGGCC AGAATGCACG GGGTCTCCTC ACCAGGCTGT TCAGCCGTGC TTTTATCTTG 
GCGGATCCAG ATGGGTCAAA TCGAATGGCA TTTGTGAGCG TGGAACTATG TATGATTTCC 
CAACGACTGA GGTTGGAGGT CCTGAAGAGA CTAGAGAGTA AATATGGCTC TCTGTATCGA 
AGAGACAATG TTATCCTGAG TGCCATTCAC ACACACTCTG GCCCAGCAGG GTTTTTCCAA 
TATACACTCT ATATACTCGC CAGCGAGGGA TTCAGCAACC GGACCTTTCA GTACATAGTC 
TCTGGGATCA TGAAGAGCAT TGATATAGCT CACACAAATC TTAAACCAGG CAAAATCTTT 
ATCAACAAAG GAAATGTTGC TAATGTGCAG ATCAACCGAA GCCCCTCCTC TTACCTTCTG 
AATCCACAGT CAGAGAGAGC AAGGTATTCT TCAAACACAG ACAAGGAAAT GCTGGTCTTG 
AAACTGGTGG ATTTGAATGG AGAAGACTTG GGTCTTATCA GCTGGTTTGC CATCCACCCC 
GTGAGCATGA ACAATAGCAA CCACTTTGTG AATAGTGACA ATATGGGCTA TGCGGCTTAC 
CTTTTTGAGC AAGAAAAGAA CAAAGGCTAT CTGCCTGGAC AGGGACCGTT TGTAGCAGGC 
TTTGCTTCAT CAAATCTCGG AGACGTGTCA CCCAACATTC TTGGCCCGCA TTGTGTCAAC 
ACAGGGGAGT CTTGTGACAA CGACAAGAGC ACCTGTCCCA ACGGTGGGCC TAGCATGTGC 
ATGGCCAGCG GACCTGGACA AGACATGTTT GAGAGCACAC ACATTATAGG ACGGATCATC 
TATCAGAAGG CCAAGGAGCT GTATGCCTCT GCCTCCCAGG AGGTGACCGG CCCAGTGCTT 
GCAGCTCACC AGTGGGTGAA CATGACAGAT GTGAGCGTCC AGCTCAATGC CACACACACA 
GTGAAGACGT GTAAACCTGC CCTGGGCTAC AGTTTTGCCG CAGGCACAAT TGATGGAGTT 
TCGGGCCTCA ATATTACACA GGGAACTACG GAAGGGGATC CATTCTGGGA CACTCTTCGG 
GACCAGCTCT TGGGAAAACC ATCTGAAGAG ATTGTAGAGT GTCAGAAACC CAAACCAATC 
CTGCTTCACA GTGGAGAGCT GACGATACCA CATCCTTGGC AACCAGATAT TGTTGATGTT 
CAGATTGTTA CCGTTGGGTC CTTGGCCATA GCTGCTATCC CTGGGGAATT AACAACCATG 
TCGGGACGAA GATTTCGTGA GGCAATTAAA AAAGAATTTG CACTTTATGG GATGAAGGAT 
ATGACCGTTG TTATCGCAGG TCTAAGCAAT GTTTATACAC ATTACATTAC CACATATGAA 
GAATACCAGG CTCAGCGGTA CGAGGCAGCA TCTACAATCT ATGGACCACA CACCCTGTCT 
GCATACATCC AACTCTTCAG AGACCTTGCT AAGGCAATTG CTACGGACAC AGTAGCCAAC 
ATGAGCAGTG GTCCCGAGCC TCCATTCTTC AAAAATCTAA TAGCTTCACT TATTCCTAAT 
ATTGCGGATA GAGCACCAAT TGGCAAACAT TTTGGGGATG TCTTGCAGCC AGCAAAACCT 
GAATACAGAG TGGGAGAAGT GGTTGAAGTT ATATTTGTAG GCGCTAACCC AAAGAATTCA 
GCAGAGAACC AGACCCATCA AACCTTCCTC ACTGTGGAGA AATACGAGGA CTCTGTAGCT 
GACTGGCAGA TAATGTATAA CGATGCCTCC TGGGAGACGA GGTTTTATTG GCACAAAGGA 
ATACTGGGTC TGAGCAATGC AACAATATAC TGGCATATTC CAGATACTGC CTACCCTGGA 
ATCTACAGAA TAAGATATTT TGGACACAAT CGGAAGCAGG AACTTCTGAA ACCCGCTGTC 
ATACTAGCAT TTGAAGGAAT TTCTTCTCCT TTTGAAGTTG TCACTACTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Asah2 ( XM_011247285.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011245587.1 Mus musculus N-acylsphingosine amidohydrolase 2 (Asah2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu10953
Clone ID Related Accession (Same CDS sequence)	XM_011247289.1 , XM_011247284.2 , XM_011247285.2 , XM_011247283.2 , XM_011247286.2 , XM_011247287.2 , XM_011247290.2 , NM_018830.1
Accession Version	XM_011247285.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2271bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	neutral ceramidase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011247285.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAAGC GAACCTTCTC CACCTTGGAG GCATTCCTCA TTTTCCTTCT GGTAATAATG 
ACAGTCATCA CAGTGGCCCT TCTCACCCTC TTGTTTGTTA CCAGTGGGAC CATTGAAAAC 
CACAAAGATT CAGGAAATCA CTGGTTTTCA ACCACTCTGG GCTCCACGAC AACCCAGCCC 
CCTCCAATTA CACAGACTCC AAACTTCCCT TCATTTCGGA ACTTCAGTGG CTACTACATT 
GGCGTTGGGA GAGCAGATTG CACAGGACAA GTGTCAGATA TCAATTTGAT GGGCTATGGC 
AAAAATGGCC AGAATGCACG GGGTCTCCTC ACCAGGCTGT TCAGCCGTGC TTTTATCTTG 
GCGGATCCAG ATGGGTCAAA TCGAATGGCA TTTGTGAGCG TGGAACTATG TATGATTTCC 
CAACGACTGA GGTTGGAGGT CCTGAAGAGA CTAGAGAGTA AATATGGCTC TCTGTATCGA 
AGAGACAATG TTATCCTGAG TGCCATTCAC ACACACTCTG GCCCAGCAGG GTTTTTCCAA 
TATACACTCT ATATACTCGC CAGCGAGGGA TTCAGCAACC GGACCTTTCA GTACATAGTC 
TCTGGGATCA TGAAGAGCAT TGATATAGCT CACACAAATC TTAAACCAGG CAAAATCTTT 
ATCAACAAAG GAAATGTTGC TAATGTGCAG ATCAACCGAA GCCCCTCCTC TTACCTTCTG 
AATCCACAGT CAGAGAGAGC AAGGTATTCT TCAAACACAG ACAAGGAAAT GCTGGTCTTG 
AAACTGGTGG ATTTGAATGG AGAAGACTTG GGTCTTATCA GCTGGTTTGC CATCCACCCC 
GTGAGCATGA ACAATAGCAA CCACTTTGTG AATAGTGACA ATATGGGCTA TGCGGCTTAC 
CTTTTTGAGC AAGAAAAGAA CAAAGGCTAT CTGCCTGGAC AGGGACCGTT TGTAGCAGGC 
TTTGCTTCAT CAAATCTCGG AGACGTGTCA CCCAACATTC TTGGCCCGCA TTGTGTCAAC 
ACAGGGGAGT CTTGTGACAA CGACAAGAGC ACCTGTCCCA ACGGTGGGCC TAGCATGTGC 
ATGGCCAGCG GACCTGGACA AGACATGTTT GAGAGCACAC ACATTATAGG ACGGATCATC 
TATCAGAAGG CCAAGGAGCT GTATGCCTCT GCCTCCCAGG AGGTGACCGG CCCAGTGCTT 
GCAGCTCACC AGTGGGTGAA CATGACAGAT GTGAGCGTCC AGCTCAATGC CACACACACA 
GTGAAGACGT GTAAACCTGC CCTGGGCTAC AGTTTTGCCG CAGGCACAAT TGATGGAGTT 
TCGGGCCTCA ATATTACACA GGGAACTACG GAAGGGGATC CATTCTGGGA CACTCTTCGG 
GACCAGCTCT TGGGAAAACC ATCTGAAGAG ATTGTAGAGT GTCAGAAACC CAAACCAATC 
CTGCTTCACA GTGGAGAGCT GACGATACCA CATCCTTGGC AACCAGATAT TGTTGATGTT 
CAGATTGTTA CCGTTGGGTC CTTGGCCATA GCTGCTATCC CTGGGGAATT AACAACCATG 
TCGGGACGAA GATTTCGTGA GGCAATTAAA AAAGAATTTG CACTTTATGG GATGAAGGAT 
ATGACCGTTG TTATCGCAGG TCTAAGCAAT GTTTATACAC ATTACATTAC CACATATGAA 
GAATACCAGG CTCAGCGGTA CGAGGCAGCA TCTACAATCT ATGGACCACA CACCCTGTCT 
GCATACATCC AACTCTTCAG AGACCTTGCT AAGGCAATTG CTACGGACAC AGTAGCCAAC 
ATGAGCAGTG GTCCCGAGCC TCCATTCTTC AAAAATCTAA TAGCTTCACT TATTCCTAAT 
ATTGCGGATA GAGCACCAAT TGGCAAACAT TTTGGGGATG TCTTGCAGCC AGCAAAACCT 
GAATACAGAG TGGGAGAAGT GGTTGAAGTT ATATTTGTAG GCGCTAACCC AAAGAATTCA 
GCAGAGAACC AGACCCATCA AACCTTCCTC ACTGTGGAGA AATACGAGGA CTCTGTAGCT 
GACTGGCAGA TAATGTATAA CGATGCCTCC TGGGAGACGA GGTTTTATTG GCACAAAGGA 
ATACTGGGTC TGAGCAATGC AACAATATAC TGGCATATTC CAGATACTGC CTACCCTGGA 
ATCTACAGAA TAAGATATTT TGGACACAAT CGGAAGCAGG AACTTCTGAA ACCCGCTGTC 
ATACTAGCAT TTGAAGGAAT TTCTTCTCCT TTTGAAGTTG TCACTACTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011245587.1
CDS	478..2748
Translation	MAKRTFSTLEAFLIFLLVIMTVITVALLTLLFVTSGTIENHKDSGNHWFSTTLGSTTTQPPPITQTPNFPSFRNFSGYYIGVGRADCTGQVSDINLMGYGKNGQNARGLLTRLFSRAFILADPDGSNRMAFVSVELCMISQRLRLEVLKRLESKYGSLYRRDNVILSAIHTHSGPAGFFQYTLYILASEGFSNRTFQYIVSGIMKSIDIAHTNLKPGKIFINKGNVANVQINRSPSSYLLNPQSERARYSSNTDKEMLVLKLVDLNGEDLGLISWFAIHPVSMNNSNHFVNSDNMGYAAYLFEQEKNKGYLPGQGPFVAGFASSNLGDVSPNILGPHCVNTGESCDNDKSTCPNGGPSMCMASGPGQDMFESTHIIGRIIYQKAKELYASASQEVTGPVLAAHQWVNMTDVSVQLNATHTVKTCKPALGYSFAAGTIDGVSGLNITQGTTEGDPFWDTLRDQLLGKPSEEIVECQKPKPILLHSGELTIPHPWQPDIVDVQIVTVGSLAIAAIPGELTTMSGRRFREAIKKEFALYGMKDMTVVIAGLSNVYTHYITTYEEYQAQRYEAASTIYGPHTLSAYIQLFRDLAKAIATDTVANMSSGPEPPFFKNLIASLIPNIADRAPIGKHFGDVLQPAKPEYRVGEVVEVIFVGANPKNSAENQTHQTFLTVEKYEDSVADWQIMYNDASWETRFYWHKGILGLSNATIYWHIPDTAYPGIYRIRYFGHNRKQELLKPAVILAFEGISSPFEVVTT

Target ORF information:

RefSeq Version	XM_011247285.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus N-acylsphingosine amidohydrolase 2 (Asah2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011247285.2

ORF Insert Sequence:

ATGGCAAAGC GAACCTTCTC CACCTTGGAG GCATTCCTCA TTTTCCTTCT GGTAATAATG 
ACAGTCATCA CAGTGGCCCT TCTCACCCTC TTGTTTGTTA CCAGTGGGAC CATTGAAAAC 
CACAAAGATT CAGGAAATCA CTGGTTTTCA ACCACTCTGG GCTCCACGAC AACCCAGCCC 
CCTCCAATTA CACAGACTCC AAACTTCCCT TCATTTCGGA ACTTCAGTGG CTACTACATT 
GGCGTTGGGA GAGCAGATTG CACAGGACAA GTGTCAGATA TCAATTTGAT GGGCTATGGC 
AAAAATGGCC AGAATGCACG GGGTCTCCTC ACCAGGCTGT TCAGCCGTGC TTTTATCTTG 
GCGGATCCAG ATGGGTCAAA TCGAATGGCA TTTGTGAGCG TGGAACTATG TATGATTTCC 
CAACGACTGA GGTTGGAGGT CCTGAAGAGA CTAGAGAGTA AATATGGCTC TCTGTATCGA 
AGAGACAATG TTATCCTGAG TGCCATTCAC ACACACTCTG GCCCAGCAGG GTTTTTCCAA 
TATACACTCT ATATACTCGC CAGCGAGGGA TTCAGCAACC GGACCTTTCA GTACATAGTC 
TCTGGGATCA TGAAGAGCAT TGATATAGCT CACACAAATC TTAAACCAGG CAAAATCTTT 
ATCAACAAAG GAAATGTTGC TAATGTGCAG ATCAACCGAA GCCCCTCCTC TTACCTTCTG 
AATCCACAGT CAGAGAGAGC AAGGTATTCT TCAAACACAG ACAAGGAAAT GCTGGTCTTG 
AAACTGGTGG ATTTGAATGG AGAAGACTTG GGTCTTATCA GCTGGTTTGC CATCCACCCC 
GTGAGCATGA ACAATAGCAA CCACTTTGTG AATAGTGACA ATATGGGCTA TGCGGCTTAC 
CTTTTTGAGC AAGAAAAGAA CAAAGGCTAT CTGCCTGGAC AGGGACCGTT TGTAGCAGGC 
TTTGCTTCAT CAAATCTCGG AGACGTGTCA CCCAACATTC TTGGCCCGCA TTGTGTCAAC 
ACAGGGGAGT CTTGTGACAA CGACAAGAGC ACCTGTCCCA ACGGTGGGCC TAGCATGTGC 
ATGGCCAGCG GACCTGGACA AGACATGTTT GAGAGCACAC ACATTATAGG ACGGATCATC 
TATCAGAAGG CCAAGGAGCT GTATGCCTCT GCCTCCCAGG AGGTGACCGG CCCAGTGCTT 
GCAGCTCACC AGTGGGTGAA CATGACAGAT GTGAGCGTCC AGCTCAATGC CACACACACA 
GTGAAGACGT GTAAACCTGC CCTGGGCTAC AGTTTTGCCG CAGGCACAAT TGATGGAGTT 
TCGGGCCTCA ATATTACACA GGGAACTACG GAAGGGGATC CATTCTGGGA CACTCTTCGG 
GACCAGCTCT TGGGAAAACC ATCTGAAGAG ATTGTAGAGT GTCAGAAACC CAAACCAATC 
CTGCTTCACA GTGGAGAGCT GACGATACCA CATCCTTGGC AACCAGATAT TGTTGATGTT 
CAGATTGTTA CCGTTGGGTC CTTGGCCATA GCTGCTATCC CTGGGGAATT AACAACCATG 
TCGGGACGAA GATTTCGTGA GGCAATTAAA AAAGAATTTG CACTTTATGG GATGAAGGAT 
ATGACCGTTG TTATCGCAGG TCTAAGCAAT GTTTATACAC ATTACATTAC CACATATGAA 
GAATACCAGG CTCAGCGGTA CGAGGCAGCA TCTACAATCT ATGGACCACA CACCCTGTCT 
GCATACATCC AACTCTTCAG AGACCTTGCT AAGGCAATTG CTACGGACAC AGTAGCCAAC 
ATGAGCAGTG GTCCCGAGCC TCCATTCTTC AAAAATCTAA TAGCTTCACT TATTCCTAAT 
ATTGCGGATA GAGCACCAAT TGGCAAACAT TTTGGGGATG TCTTGCAGCC AGCAAAACCT 
GAATACAGAG TGGGAGAAGT GGTTGAAGTT ATATTTGTAG GCGCTAACCC AAAGAATTCA 
GCAGAGAACC AGACCCATCA AACCTTCCTC ACTGTGGAGA AATACGAGGA CTCTGTAGCT 
GACTGGCAGA TAATGTATAA CGATGCCTCC TGGGAGACGA GGTTTTATTG GCACAAAGGA 
ATACTGGGTC TGAGCAATGC AACAATATAC TGGCATATTC CAGATACTGC CTACCCTGGA 
ATCTACAGAA TAAGATATTT TGGACACAAT CGGAAGCAGG AACTTCTGAA ACCCGCTGTC 
ATACTAGCAT TTGAAGGAAT TTCTTCTCCT TTTGAAGTTG TCACTACTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Asah2 ( XM_011247283.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011245585.1 Mus musculus N-acylsphingosine amidohydrolase 2 (Asah2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu10953
Clone ID Related Accession (Same CDS sequence)	XM_011247289.1 , XM_011247284.2 , XM_011247285.2 , XM_011247283.2 , XM_011247286.2 , XM_011247287.2 , XM_011247290.2 , NM_018830.1
Accession Version	XM_011247283.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2271bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	neutral ceramidase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011247283.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAAGC GAACCTTCTC CACCTTGGAG GCATTCCTCA TTTTCCTTCT GGTAATAATG 
ACAGTCATCA CAGTGGCCCT TCTCACCCTC TTGTTTGTTA CCAGTGGGAC CATTGAAAAC 
CACAAAGATT CAGGAAATCA CTGGTTTTCA ACCACTCTGG GCTCCACGAC AACCCAGCCC 
CCTCCAATTA CACAGACTCC AAACTTCCCT TCATTTCGGA ACTTCAGTGG CTACTACATT 
GGCGTTGGGA GAGCAGATTG CACAGGACAA GTGTCAGATA TCAATTTGAT GGGCTATGGC 
AAAAATGGCC AGAATGCACG GGGTCTCCTC ACCAGGCTGT TCAGCCGTGC TTTTATCTTG 
GCGGATCCAG ATGGGTCAAA TCGAATGGCA TTTGTGAGCG TGGAACTATG TATGATTTCC 
CAACGACTGA GGTTGGAGGT CCTGAAGAGA CTAGAGAGTA AATATGGCTC TCTGTATCGA 
AGAGACAATG TTATCCTGAG TGCCATTCAC ACACACTCTG GCCCAGCAGG GTTTTTCCAA 
TATACACTCT ATATACTCGC CAGCGAGGGA TTCAGCAACC GGACCTTTCA GTACATAGTC 
TCTGGGATCA TGAAGAGCAT TGATATAGCT CACACAAATC TTAAACCAGG CAAAATCTTT 
ATCAACAAAG GAAATGTTGC TAATGTGCAG ATCAACCGAA GCCCCTCCTC TTACCTTCTG 
AATCCACAGT CAGAGAGAGC AAGGTATTCT TCAAACACAG ACAAGGAAAT GCTGGTCTTG 
AAACTGGTGG ATTTGAATGG AGAAGACTTG GGTCTTATCA GCTGGTTTGC CATCCACCCC 
GTGAGCATGA ACAATAGCAA CCACTTTGTG AATAGTGACA ATATGGGCTA TGCGGCTTAC 
CTTTTTGAGC AAGAAAAGAA CAAAGGCTAT CTGCCTGGAC AGGGACCGTT TGTAGCAGGC 
TTTGCTTCAT CAAATCTCGG AGACGTGTCA CCCAACATTC TTGGCCCGCA TTGTGTCAAC 
ACAGGGGAGT CTTGTGACAA CGACAAGAGC ACCTGTCCCA ACGGTGGGCC TAGCATGTGC 
ATGGCCAGCG GACCTGGACA AGACATGTTT GAGAGCACAC ACATTATAGG ACGGATCATC 
TATCAGAAGG CCAAGGAGCT GTATGCCTCT GCCTCCCAGG AGGTGACCGG CCCAGTGCTT 
GCAGCTCACC AGTGGGTGAA CATGACAGAT GTGAGCGTCC AGCTCAATGC CACACACACA 
GTGAAGACGT GTAAACCTGC CCTGGGCTAC AGTTTTGCCG CAGGCACAAT TGATGGAGTT 
TCGGGCCTCA ATATTACACA GGGAACTACG GAAGGGGATC CATTCTGGGA CACTCTTCGG 
GACCAGCTCT TGGGAAAACC ATCTGAAGAG ATTGTAGAGT GTCAGAAACC CAAACCAATC 
CTGCTTCACA GTGGAGAGCT GACGATACCA CATCCTTGGC AACCAGATAT TGTTGATGTT 
CAGATTGTTA CCGTTGGGTC CTTGGCCATA GCTGCTATCC CTGGGGAATT AACAACCATG 
TCGGGACGAA GATTTCGTGA GGCAATTAAA AAAGAATTTG CACTTTATGG GATGAAGGAT 
ATGACCGTTG TTATCGCAGG TCTAAGCAAT GTTTATACAC ATTACATTAC CACATATGAA 
GAATACCAGG CTCAGCGGTA CGAGGCAGCA TCTACAATCT ATGGACCACA CACCCTGTCT 
GCATACATCC AACTCTTCAG AGACCTTGCT AAGGCAATTG CTACGGACAC AGTAGCCAAC 
ATGAGCAGTG GTCCCGAGCC TCCATTCTTC AAAAATCTAA TAGCTTCACT TATTCCTAAT 
ATTGCGGATA GAGCACCAAT TGGCAAACAT TTTGGGGATG TCTTGCAGCC AGCAAAACCT 
GAATACAGAG TGGGAGAAGT GGTTGAAGTT ATATTTGTAG GCGCTAACCC AAAGAATTCA 
GCAGAGAACC AGACCCATCA AACCTTCCTC ACTGTGGAGA AATACGAGGA CTCTGTAGCT 
GACTGGCAGA TAATGTATAA CGATGCCTCC TGGGAGACGA GGTTTTATTG GCACAAAGGA 
ATACTGGGTC TGAGCAATGC AACAATATAC TGGCATATTC CAGATACTGC CTACCCTGGA 
ATCTACAGAA TAAGATATTT TGGACACAAT CGGAAGCAGG AACTTCTGAA ACCCGCTGTC 
ATACTAGCAT TTGAAGGAAT TTCTTCTCCT TTTGAAGTTG TCACTACTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011245585.1
CDS	762..3032
Translation	MAKRTFSTLEAFLIFLLVIMTVITVALLTLLFVTSGTIENHKDSGNHWFSTTLGSTTTQPPPITQTPNFPSFRNFSGYYIGVGRADCTGQVSDINLMGYGKNGQNARGLLTRLFSRAFILADPDGSNRMAFVSVELCMISQRLRLEVLKRLESKYGSLYRRDNVILSAIHTHSGPAGFFQYTLYILASEGFSNRTFQYIVSGIMKSIDIAHTNLKPGKIFINKGNVANVQINRSPSSYLLNPQSERARYSSNTDKEMLVLKLVDLNGEDLGLISWFAIHPVSMNNSNHFVNSDNMGYAAYLFEQEKNKGYLPGQGPFVAGFASSNLGDVSPNILGPHCVNTGESCDNDKSTCPNGGPSMCMASGPGQDMFESTHIIGRIIYQKAKELYASASQEVTGPVLAAHQWVNMTDVSVQLNATHTVKTCKPALGYSFAAGTIDGVSGLNITQGTTEGDPFWDTLRDQLLGKPSEEIVECQKPKPILLHSGELTIPHPWQPDIVDVQIVTVGSLAIAAIPGELTTMSGRRFREAIKKEFALYGMKDMTVVIAGLSNVYTHYITTYEEYQAQRYEAASTIYGPHTLSAYIQLFRDLAKAIATDTVANMSSGPEPPFFKNLIASLIPNIADRAPIGKHFGDVLQPAKPEYRVGEVVEVIFVGANPKNSAENQTHQTFLTVEKYEDSVADWQIMYNDASWETRFYWHKGILGLSNATIYWHIPDTAYPGIYRIRYFGHNRKQELLKPAVILAFEGISSPFEVVTT

Target ORF information:

RefSeq Version	XM_011247283.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus N-acylsphingosine amidohydrolase 2 (Asah2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011247283.2

ORF Insert Sequence:

ATGGCAAAGC GAACCTTCTC CACCTTGGAG GCATTCCTCA TTTTCCTTCT GGTAATAATG 
ACAGTCATCA CAGTGGCCCT TCTCACCCTC TTGTTTGTTA CCAGTGGGAC CATTGAAAAC 
CACAAAGATT CAGGAAATCA CTGGTTTTCA ACCACTCTGG GCTCCACGAC AACCCAGCCC 
CCTCCAATTA CACAGACTCC AAACTTCCCT TCATTTCGGA ACTTCAGTGG CTACTACATT 
GGCGTTGGGA GAGCAGATTG CACAGGACAA GTGTCAGATA TCAATTTGAT GGGCTATGGC 
AAAAATGGCC AGAATGCACG GGGTCTCCTC ACCAGGCTGT TCAGCCGTGC TTTTATCTTG 
GCGGATCCAG ATGGGTCAAA TCGAATGGCA TTTGTGAGCG TGGAACTATG TATGATTTCC 
CAACGACTGA GGTTGGAGGT CCTGAAGAGA CTAGAGAGTA AATATGGCTC TCTGTATCGA 
AGAGACAATG TTATCCTGAG TGCCATTCAC ACACACTCTG GCCCAGCAGG GTTTTTCCAA 
TATACACTCT ATATACTCGC CAGCGAGGGA TTCAGCAACC GGACCTTTCA GTACATAGTC 
TCTGGGATCA TGAAGAGCAT TGATATAGCT CACACAAATC TTAAACCAGG CAAAATCTTT 
ATCAACAAAG GAAATGTTGC TAATGTGCAG ATCAACCGAA GCCCCTCCTC TTACCTTCTG 
AATCCACAGT CAGAGAGAGC AAGGTATTCT TCAAACACAG ACAAGGAAAT GCTGGTCTTG 
AAACTGGTGG ATTTGAATGG AGAAGACTTG GGTCTTATCA GCTGGTTTGC CATCCACCCC 
GTGAGCATGA ACAATAGCAA CCACTTTGTG AATAGTGACA ATATGGGCTA TGCGGCTTAC 
CTTTTTGAGC AAGAAAAGAA CAAAGGCTAT CTGCCTGGAC AGGGACCGTT TGTAGCAGGC 
TTTGCTTCAT CAAATCTCGG AGACGTGTCA CCCAACATTC TTGGCCCGCA TTGTGTCAAC 
ACAGGGGAGT CTTGTGACAA CGACAAGAGC ACCTGTCCCA ACGGTGGGCC TAGCATGTGC 
ATGGCCAGCG GACCTGGACA AGACATGTTT GAGAGCACAC ACATTATAGG ACGGATCATC 
TATCAGAAGG CCAAGGAGCT GTATGCCTCT GCCTCCCAGG AGGTGACCGG CCCAGTGCTT 
GCAGCTCACC AGTGGGTGAA CATGACAGAT GTGAGCGTCC AGCTCAATGC CACACACACA 
GTGAAGACGT GTAAACCTGC CCTGGGCTAC AGTTTTGCCG CAGGCACAAT TGATGGAGTT 
TCGGGCCTCA ATATTACACA GGGAACTACG GAAGGGGATC CATTCTGGGA CACTCTTCGG 
GACCAGCTCT TGGGAAAACC ATCTGAAGAG ATTGTAGAGT GTCAGAAACC CAAACCAATC 
CTGCTTCACA GTGGAGAGCT GACGATACCA CATCCTTGGC AACCAGATAT TGTTGATGTT 
CAGATTGTTA CCGTTGGGTC CTTGGCCATA GCTGCTATCC CTGGGGAATT AACAACCATG 
TCGGGACGAA GATTTCGTGA GGCAATTAAA AAAGAATTTG CACTTTATGG GATGAAGGAT 
ATGACCGTTG TTATCGCAGG TCTAAGCAAT GTTTATACAC ATTACATTAC CACATATGAA 
GAATACCAGG CTCAGCGGTA CGAGGCAGCA TCTACAATCT ATGGACCACA CACCCTGTCT 
GCATACATCC AACTCTTCAG AGACCTTGCT AAGGCAATTG CTACGGACAC AGTAGCCAAC 
ATGAGCAGTG GTCCCGAGCC TCCATTCTTC AAAAATCTAA TAGCTTCACT TATTCCTAAT 
ATTGCGGATA GAGCACCAAT TGGCAAACAT TTTGGGGATG TCTTGCAGCC AGCAAAACCT 
GAATACAGAG TGGGAGAAGT GGTTGAAGTT ATATTTGTAG GCGCTAACCC AAAGAATTCA 
GCAGAGAACC AGACCCATCA AACCTTCCTC ACTGTGGAGA AATACGAGGA CTCTGTAGCT 
GACTGGCAGA TAATGTATAA CGATGCCTCC TGGGAGACGA GGTTTTATTG GCACAAAGGA 
ATACTGGGTC TGAGCAATGC AACAATATAC TGGCATATTC CAGATACTGC CTACCCTGGA 
ATCTACAGAA TAAGATATTT TGGACACAAT CGGAAGCAGG AACTTCTGAA ACCCGCTGTC 
ATACTAGCAT TTGAAGGAAT TTCTTCTCCT TTTGAAGTTG TCACTACTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Asah2 ( XM_011247286.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011245588.1 Mus musculus N-acylsphingosine amidohydrolase 2 (Asah2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu10953
Clone ID Related Accession (Same CDS sequence)	XM_011247289.1 , XM_011247284.2 , XM_011247285.2 , XM_011247283.2 , XM_011247286.2 , XM_011247287.2 , XM_011247290.2 , NM_018830.1
Accession Version	XM_011247286.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2271bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	neutral ceramidase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011247286.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAAGC GAACCTTCTC CACCTTGGAG GCATTCCTCA TTTTCCTTCT GGTAATAATG 
ACAGTCATCA CAGTGGCCCT TCTCACCCTC TTGTTTGTTA CCAGTGGGAC CATTGAAAAC 
CACAAAGATT CAGGAAATCA CTGGTTTTCA ACCACTCTGG GCTCCACGAC AACCCAGCCC 
CCTCCAATTA CACAGACTCC AAACTTCCCT TCATTTCGGA ACTTCAGTGG CTACTACATT 
GGCGTTGGGA GAGCAGATTG CACAGGACAA GTGTCAGATA TCAATTTGAT GGGCTATGGC 
AAAAATGGCC AGAATGCACG GGGTCTCCTC ACCAGGCTGT TCAGCCGTGC TTTTATCTTG 
GCGGATCCAG ATGGGTCAAA TCGAATGGCA TTTGTGAGCG TGGAACTATG TATGATTTCC 
CAACGACTGA GGTTGGAGGT CCTGAAGAGA CTAGAGAGTA AATATGGCTC TCTGTATCGA 
AGAGACAATG TTATCCTGAG TGCCATTCAC ACACACTCTG GCCCAGCAGG GTTTTTCCAA 
TATACACTCT ATATACTCGC CAGCGAGGGA TTCAGCAACC GGACCTTTCA GTACATAGTC 
TCTGGGATCA TGAAGAGCAT TGATATAGCT CACACAAATC TTAAACCAGG CAAAATCTTT 
ATCAACAAAG GAAATGTTGC TAATGTGCAG ATCAACCGAA GCCCCTCCTC TTACCTTCTG 
AATCCACAGT CAGAGAGAGC AAGGTATTCT TCAAACACAG ACAAGGAAAT GCTGGTCTTG 
AAACTGGTGG ATTTGAATGG AGAAGACTTG GGTCTTATCA GCTGGTTTGC CATCCACCCC 
GTGAGCATGA ACAATAGCAA CCACTTTGTG AATAGTGACA ATATGGGCTA TGCGGCTTAC 
CTTTTTGAGC AAGAAAAGAA CAAAGGCTAT CTGCCTGGAC AGGGACCGTT TGTAGCAGGC 
TTTGCTTCAT CAAATCTCGG AGACGTGTCA CCCAACATTC TTGGCCCGCA TTGTGTCAAC 
ACAGGGGAGT CTTGTGACAA CGACAAGAGC ACCTGTCCCA ACGGTGGGCC TAGCATGTGC 
ATGGCCAGCG GACCTGGACA AGACATGTTT GAGAGCACAC ACATTATAGG ACGGATCATC 
TATCAGAAGG CCAAGGAGCT GTATGCCTCT GCCTCCCAGG AGGTGACCGG CCCAGTGCTT 
GCAGCTCACC AGTGGGTGAA CATGACAGAT GTGAGCGTCC AGCTCAATGC CACACACACA 
GTGAAGACGT GTAAACCTGC CCTGGGCTAC AGTTTTGCCG CAGGCACAAT TGATGGAGTT 
TCGGGCCTCA ATATTACACA GGGAACTACG GAAGGGGATC CATTCTGGGA CACTCTTCGG 
GACCAGCTCT TGGGAAAACC ATCTGAAGAG ATTGTAGAGT GTCAGAAACC CAAACCAATC 
CTGCTTCACA GTGGAGAGCT GACGATACCA CATCCTTGGC AACCAGATAT TGTTGATGTT 
CAGATTGTTA CCGTTGGGTC CTTGGCCATA GCTGCTATCC CTGGGGAATT AACAACCATG 
TCGGGACGAA GATTTCGTGA GGCAATTAAA AAAGAATTTG CACTTTATGG GATGAAGGAT 
ATGACCGTTG TTATCGCAGG TCTAAGCAAT GTTTATACAC ATTACATTAC CACATATGAA 
GAATACCAGG CTCAGCGGTA CGAGGCAGCA TCTACAATCT ATGGACCACA CACCCTGTCT 
GCATACATCC AACTCTTCAG AGACCTTGCT AAGGCAATTG CTACGGACAC AGTAGCCAAC 
ATGAGCAGTG GTCCCGAGCC TCCATTCTTC AAAAATCTAA TAGCTTCACT TATTCCTAAT 
ATTGCGGATA GAGCACCAAT TGGCAAACAT TTTGGGGATG TCTTGCAGCC AGCAAAACCT 
GAATACAGAG TGGGAGAAGT GGTTGAAGTT ATATTTGTAG GCGCTAACCC AAAGAATTCA 
GCAGAGAACC AGACCCATCA AACCTTCCTC ACTGTGGAGA AATACGAGGA CTCTGTAGCT 
GACTGGCAGA TAATGTATAA CGATGCCTCC TGGGAGACGA GGTTTTATTG GCACAAAGGA 
ATACTGGGTC TGAGCAATGC AACAATATAC TGGCATATTC CAGATACTGC CTACCCTGGA 
ATCTACAGAA TAAGATATTT TGGACACAAT CGGAAGCAGG AACTTCTGAA ACCCGCTGTC 
ATACTAGCAT TTGAAGGAAT TTCTTCTCCT TTTGAAGTTG TCACTACTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011245588.1
CDS	797..3067
Translation	MAKRTFSTLEAFLIFLLVIMTVITVALLTLLFVTSGTIENHKDSGNHWFSTTLGSTTTQPPPITQTPNFPSFRNFSGYYIGVGRADCTGQVSDINLMGYGKNGQNARGLLTRLFSRAFILADPDGSNRMAFVSVELCMISQRLRLEVLKRLESKYGSLYRRDNVILSAIHTHSGPAGFFQYTLYILASEGFSNRTFQYIVSGIMKSIDIAHTNLKPGKIFINKGNVANVQINRSPSSYLLNPQSERARYSSNTDKEMLVLKLVDLNGEDLGLISWFAIHPVSMNNSNHFVNSDNMGYAAYLFEQEKNKGYLPGQGPFVAGFASSNLGDVSPNILGPHCVNTGESCDNDKSTCPNGGPSMCMASGPGQDMFESTHIIGRIIYQKAKELYASASQEVTGPVLAAHQWVNMTDVSVQLNATHTVKTCKPALGYSFAAGTIDGVSGLNITQGTTEGDPFWDTLRDQLLGKPSEEIVECQKPKPILLHSGELTIPHPWQPDIVDVQIVTVGSLAIAAIPGELTTMSGRRFREAIKKEFALYGMKDMTVVIAGLSNVYTHYITTYEEYQAQRYEAASTIYGPHTLSAYIQLFRDLAKAIATDTVANMSSGPEPPFFKNLIASLIPNIADRAPIGKHFGDVLQPAKPEYRVGEVVEVIFVGANPKNSAENQTHQTFLTVEKYEDSVADWQIMYNDASWETRFYWHKGILGLSNATIYWHIPDTAYPGIYRIRYFGHNRKQELLKPAVILAFEGISSPFEVVTT

Target ORF information:

RefSeq Version	XM_011247286.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus N-acylsphingosine amidohydrolase 2 (Asah2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011247286.2

ORF Insert Sequence:

ATGGCAAAGC GAACCTTCTC CACCTTGGAG GCATTCCTCA TTTTCCTTCT GGTAATAATG 
ACAGTCATCA CAGTGGCCCT TCTCACCCTC TTGTTTGTTA CCAGTGGGAC CATTGAAAAC 
CACAAAGATT CAGGAAATCA CTGGTTTTCA ACCACTCTGG GCTCCACGAC AACCCAGCCC 
CCTCCAATTA CACAGACTCC AAACTTCCCT TCATTTCGGA ACTTCAGTGG CTACTACATT 
GGCGTTGGGA GAGCAGATTG CACAGGACAA GTGTCAGATA TCAATTTGAT GGGCTATGGC 
AAAAATGGCC AGAATGCACG GGGTCTCCTC ACCAGGCTGT TCAGCCGTGC TTTTATCTTG 
GCGGATCCAG ATGGGTCAAA TCGAATGGCA TTTGTGAGCG TGGAACTATG TATGATTTCC 
CAACGACTGA GGTTGGAGGT CCTGAAGAGA CTAGAGAGTA AATATGGCTC TCTGTATCGA 
AGAGACAATG TTATCCTGAG TGCCATTCAC ACACACTCTG GCCCAGCAGG GTTTTTCCAA 
TATACACTCT ATATACTCGC CAGCGAGGGA TTCAGCAACC GGACCTTTCA GTACATAGTC 
TCTGGGATCA TGAAGAGCAT TGATATAGCT CACACAAATC TTAAACCAGG CAAAATCTTT 
ATCAACAAAG GAAATGTTGC TAATGTGCAG ATCAACCGAA GCCCCTCCTC TTACCTTCTG 
AATCCACAGT CAGAGAGAGC AAGGTATTCT TCAAACACAG ACAAGGAAAT GCTGGTCTTG 
AAACTGGTGG ATTTGAATGG AGAAGACTTG GGTCTTATCA GCTGGTTTGC CATCCACCCC 
GTGAGCATGA ACAATAGCAA CCACTTTGTG AATAGTGACA ATATGGGCTA TGCGGCTTAC 
CTTTTTGAGC AAGAAAAGAA CAAAGGCTAT CTGCCTGGAC AGGGACCGTT TGTAGCAGGC 
TTTGCTTCAT CAAATCTCGG AGACGTGTCA CCCAACATTC TTGGCCCGCA TTGTGTCAAC 
ACAGGGGAGT CTTGTGACAA CGACAAGAGC ACCTGTCCCA ACGGTGGGCC TAGCATGTGC 
ATGGCCAGCG GACCTGGACA AGACATGTTT GAGAGCACAC ACATTATAGG ACGGATCATC 
TATCAGAAGG CCAAGGAGCT GTATGCCTCT GCCTCCCAGG AGGTGACCGG CCCAGTGCTT 
GCAGCTCACC AGTGGGTGAA CATGACAGAT GTGAGCGTCC AGCTCAATGC CACACACACA 
GTGAAGACGT GTAAACCTGC CCTGGGCTAC AGTTTTGCCG CAGGCACAAT TGATGGAGTT 
TCGGGCCTCA ATATTACACA GGGAACTACG GAAGGGGATC CATTCTGGGA CACTCTTCGG 
GACCAGCTCT TGGGAAAACC ATCTGAAGAG ATTGTAGAGT GTCAGAAACC CAAACCAATC 
CTGCTTCACA GTGGAGAGCT GACGATACCA CATCCTTGGC AACCAGATAT TGTTGATGTT 
CAGATTGTTA CCGTTGGGTC CTTGGCCATA GCTGCTATCC CTGGGGAATT AACAACCATG 
TCGGGACGAA GATTTCGTGA GGCAATTAAA AAAGAATTTG CACTTTATGG GATGAAGGAT 
ATGACCGTTG TTATCGCAGG TCTAAGCAAT GTTTATACAC ATTACATTAC CACATATGAA 
GAATACCAGG CTCAGCGGTA CGAGGCAGCA TCTACAATCT ATGGACCACA CACCCTGTCT 
GCATACATCC AACTCTTCAG AGACCTTGCT AAGGCAATTG CTACGGACAC AGTAGCCAAC 
ATGAGCAGTG GTCCCGAGCC TCCATTCTTC AAAAATCTAA TAGCTTCACT TATTCCTAAT 
ATTGCGGATA GAGCACCAAT TGGCAAACAT TTTGGGGATG TCTTGCAGCC AGCAAAACCT 
GAATACAGAG TGGGAGAAGT GGTTGAAGTT ATATTTGTAG GCGCTAACCC AAAGAATTCA 
GCAGAGAACC AGACCCATCA AACCTTCCTC ACTGTGGAGA AATACGAGGA CTCTGTAGCT 
GACTGGCAGA TAATGTATAA CGATGCCTCC TGGGAGACGA GGTTTTATTG GCACAAAGGA 
ATACTGGGTC TGAGCAATGC AACAATATAC TGGCATATTC CAGATACTGC CTACCCTGGA 
ATCTACAGAA TAAGATATTT TGGACACAAT CGGAAGCAGG AACTTCTGAA ACCCGCTGTC 
ATACTAGCAT TTGAAGGAAT TTCTTCTCCT TTTGAAGTTG TCACTACTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Asah2 ( XM_011247287.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011245589.1 Mus musculus N-acylsphingosine amidohydrolase 2 (Asah2), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OMu10953
Clone ID Related Accession (Same CDS sequence)	XM_011247289.1 , XM_011247284.2 , XM_011247285.2 , XM_011247283.2 , XM_011247286.2 , XM_011247287.2 , XM_011247290.2 , NM_018830.1
Accession Version	XM_011247287.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2271bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	neutral ceramidase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011247287.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAAGC GAACCTTCTC CACCTTGGAG GCATTCCTCA TTTTCCTTCT GGTAATAATG 
ACAGTCATCA CAGTGGCCCT TCTCACCCTC TTGTTTGTTA CCAGTGGGAC CATTGAAAAC 
CACAAAGATT CAGGAAATCA CTGGTTTTCA ACCACTCTGG GCTCCACGAC AACCCAGCCC 
CCTCCAATTA CACAGACTCC AAACTTCCCT TCATTTCGGA ACTTCAGTGG CTACTACATT 
GGCGTTGGGA GAGCAGATTG CACAGGACAA GTGTCAGATA TCAATTTGAT GGGCTATGGC 
AAAAATGGCC AGAATGCACG GGGTCTCCTC ACCAGGCTGT TCAGCCGTGC TTTTATCTTG 
GCGGATCCAG ATGGGTCAAA TCGAATGGCA TTTGTGAGCG TGGAACTATG TATGATTTCC 
CAACGACTGA GGTTGGAGGT CCTGAAGAGA CTAGAGAGTA AATATGGCTC TCTGTATCGA 
AGAGACAATG TTATCCTGAG TGCCATTCAC ACACACTCTG GCCCAGCAGG GTTTTTCCAA 
TATACACTCT ATATACTCGC CAGCGAGGGA TTCAGCAACC GGACCTTTCA GTACATAGTC 
TCTGGGATCA TGAAGAGCAT TGATATAGCT CACACAAATC TTAAACCAGG CAAAATCTTT 
ATCAACAAAG GAAATGTTGC TAATGTGCAG ATCAACCGAA GCCCCTCCTC TTACCTTCTG 
AATCCACAGT CAGAGAGAGC AAGGTATTCT TCAAACACAG ACAAGGAAAT GCTGGTCTTG 
AAACTGGTGG ATTTGAATGG AGAAGACTTG GGTCTTATCA GCTGGTTTGC CATCCACCCC 
GTGAGCATGA ACAATAGCAA CCACTTTGTG AATAGTGACA ATATGGGCTA TGCGGCTTAC 
CTTTTTGAGC AAGAAAAGAA CAAAGGCTAT CTGCCTGGAC AGGGACCGTT TGTAGCAGGC 
TTTGCTTCAT CAAATCTCGG AGACGTGTCA CCCAACATTC TTGGCCCGCA TTGTGTCAAC 
ACAGGGGAGT CTTGTGACAA CGACAAGAGC ACCTGTCCCA ACGGTGGGCC TAGCATGTGC 
ATGGCCAGCG GACCTGGACA AGACATGTTT GAGAGCACAC ACATTATAGG ACGGATCATC 
TATCAGAAGG CCAAGGAGCT GTATGCCTCT GCCTCCCAGG AGGTGACCGG CCCAGTGCTT 
GCAGCTCACC AGTGGGTGAA CATGACAGAT GTGAGCGTCC AGCTCAATGC CACACACACA 
GTGAAGACGT GTAAACCTGC CCTGGGCTAC AGTTTTGCCG CAGGCACAAT TGATGGAGTT 
TCGGGCCTCA ATATTACACA GGGAACTACG GAAGGGGATC CATTCTGGGA CACTCTTCGG 
GACCAGCTCT TGGGAAAACC ATCTGAAGAG ATTGTAGAGT GTCAGAAACC CAAACCAATC 
CTGCTTCACA GTGGAGAGCT GACGATACCA CATCCTTGGC AACCAGATAT TGTTGATGTT 
CAGATTGTTA CCGTTGGGTC CTTGGCCATA GCTGCTATCC CTGGGGAATT AACAACCATG 
TCGGGACGAA GATTTCGTGA GGCAATTAAA AAAGAATTTG CACTTTATGG GATGAAGGAT 
ATGACCGTTG TTATCGCAGG TCTAAGCAAT GTTTATACAC ATTACATTAC CACATATGAA 
GAATACCAGG CTCAGCGGTA CGAGGCAGCA TCTACAATCT ATGGACCACA CACCCTGTCT 
GCATACATCC AACTCTTCAG AGACCTTGCT AAGGCAATTG CTACGGACAC AGTAGCCAAC 
ATGAGCAGTG GTCCCGAGCC TCCATTCTTC AAAAATCTAA TAGCTTCACT TATTCCTAAT 
ATTGCGGATA GAGCACCAAT TGGCAAACAT TTTGGGGATG TCTTGCAGCC AGCAAAACCT 
GAATACAGAG TGGGAGAAGT GGTTGAAGTT ATATTTGTAG GCGCTAACCC AAAGAATTCA 
GCAGAGAACC AGACCCATCA AACCTTCCTC ACTGTGGAGA AATACGAGGA CTCTGTAGCT 
GACTGGCAGA TAATGTATAA CGATGCCTCC TGGGAGACGA GGTTTTATTG GCACAAAGGA 
ATACTGGGTC TGAGCAATGC AACAATATAC TGGCATATTC CAGATACTGC CTACCCTGGA 
ATCTACAGAA TAAGATATTT TGGACACAAT CGGAAGCAGG AACTTCTGAA ACCCGCTGTC 
ATACTAGCAT TTGAAGGAAT TTCTTCTCCT TTTGAAGTTG TCACTACTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011245589.1
CDS	704..2974
Translation	MAKRTFSTLEAFLIFLLVIMTVITVALLTLLFVTSGTIENHKDSGNHWFSTTLGSTTTQPPPITQTPNFPSFRNFSGYYIGVGRADCTGQVSDINLMGYGKNGQNARGLLTRLFSRAFILADPDGSNRMAFVSVELCMISQRLRLEVLKRLESKYGSLYRRDNVILSAIHTHSGPAGFFQYTLYILASEGFSNRTFQYIVSGIMKSIDIAHTNLKPGKIFINKGNVANVQINRSPSSYLLNPQSERARYSSNTDKEMLVLKLVDLNGEDLGLISWFAIHPVSMNNSNHFVNSDNMGYAAYLFEQEKNKGYLPGQGPFVAGFASSNLGDVSPNILGPHCVNTGESCDNDKSTCPNGGPSMCMASGPGQDMFESTHIIGRIIYQKAKELYASASQEVTGPVLAAHQWVNMTDVSVQLNATHTVKTCKPALGYSFAAGTIDGVSGLNITQGTTEGDPFWDTLRDQLLGKPSEEIVECQKPKPILLHSGELTIPHPWQPDIVDVQIVTVGSLAIAAIPGELTTMSGRRFREAIKKEFALYGMKDMTVVIAGLSNVYTHYITTYEEYQAQRYEAASTIYGPHTLSAYIQLFRDLAKAIATDTVANMSSGPEPPFFKNLIASLIPNIADRAPIGKHFGDVLQPAKPEYRVGEVVEVIFVGANPKNSAENQTHQTFLTVEKYEDSVADWQIMYNDASWETRFYWHKGILGLSNATIYWHIPDTAYPGIYRIRYFGHNRKQELLKPAVILAFEGISSPFEVVTT

Target ORF information:

RefSeq Version	XM_011247287.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus N-acylsphingosine amidohydrolase 2 (Asah2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011247287.2

ORF Insert Sequence:

ATGGCAAAGC GAACCTTCTC CACCTTGGAG GCATTCCTCA TTTTCCTTCT GGTAATAATG 
ACAGTCATCA CAGTGGCCCT TCTCACCCTC TTGTTTGTTA CCAGTGGGAC CATTGAAAAC 
CACAAAGATT CAGGAAATCA CTGGTTTTCA ACCACTCTGG GCTCCACGAC AACCCAGCCC 
CCTCCAATTA CACAGACTCC AAACTTCCCT TCATTTCGGA ACTTCAGTGG CTACTACATT 
GGCGTTGGGA GAGCAGATTG CACAGGACAA GTGTCAGATA TCAATTTGAT GGGCTATGGC 
AAAAATGGCC AGAATGCACG GGGTCTCCTC ACCAGGCTGT TCAGCCGTGC TTTTATCTTG 
GCGGATCCAG ATGGGTCAAA TCGAATGGCA TTTGTGAGCG TGGAACTATG TATGATTTCC 
CAACGACTGA GGTTGGAGGT CCTGAAGAGA CTAGAGAGTA AATATGGCTC TCTGTATCGA 
AGAGACAATG TTATCCTGAG TGCCATTCAC ACACACTCTG GCCCAGCAGG GTTTTTCCAA 
TATACACTCT ATATACTCGC CAGCGAGGGA TTCAGCAACC GGACCTTTCA GTACATAGTC 
TCTGGGATCA TGAAGAGCAT TGATATAGCT CACACAAATC TTAAACCAGG CAAAATCTTT 
ATCAACAAAG GAAATGTTGC TAATGTGCAG ATCAACCGAA GCCCCTCCTC TTACCTTCTG 
AATCCACAGT CAGAGAGAGC AAGGTATTCT TCAAACACAG ACAAGGAAAT GCTGGTCTTG 
AAACTGGTGG ATTTGAATGG AGAAGACTTG GGTCTTATCA GCTGGTTTGC CATCCACCCC 
GTGAGCATGA ACAATAGCAA CCACTTTGTG AATAGTGACA ATATGGGCTA TGCGGCTTAC 
CTTTTTGAGC AAGAAAAGAA CAAAGGCTAT CTGCCTGGAC AGGGACCGTT TGTAGCAGGC 
TTTGCTTCAT CAAATCTCGG AGACGTGTCA CCCAACATTC TTGGCCCGCA TTGTGTCAAC 
ACAGGGGAGT CTTGTGACAA CGACAAGAGC ACCTGTCCCA ACGGTGGGCC TAGCATGTGC 
ATGGCCAGCG GACCTGGACA AGACATGTTT GAGAGCACAC ACATTATAGG ACGGATCATC 
TATCAGAAGG CCAAGGAGCT GTATGCCTCT GCCTCCCAGG AGGTGACCGG CCCAGTGCTT 
GCAGCTCACC AGTGGGTGAA CATGACAGAT GTGAGCGTCC AGCTCAATGC CACACACACA 
GTGAAGACGT GTAAACCTGC CCTGGGCTAC AGTTTTGCCG CAGGCACAAT TGATGGAGTT 
TCGGGCCTCA ATATTACACA GGGAACTACG GAAGGGGATC CATTCTGGGA CACTCTTCGG 
GACCAGCTCT TGGGAAAACC ATCTGAAGAG ATTGTAGAGT GTCAGAAACC CAAACCAATC 
CTGCTTCACA GTGGAGAGCT GACGATACCA CATCCTTGGC AACCAGATAT TGTTGATGTT 
CAGATTGTTA CCGTTGGGTC CTTGGCCATA GCTGCTATCC CTGGGGAATT AACAACCATG 
TCGGGACGAA GATTTCGTGA GGCAATTAAA AAAGAATTTG CACTTTATGG GATGAAGGAT 
ATGACCGTTG TTATCGCAGG TCTAAGCAAT GTTTATACAC ATTACATTAC CACATATGAA 
GAATACCAGG CTCAGCGGTA CGAGGCAGCA TCTACAATCT ATGGACCACA CACCCTGTCT 
GCATACATCC AACTCTTCAG AGACCTTGCT AAGGCAATTG CTACGGACAC AGTAGCCAAC 
ATGAGCAGTG GTCCCGAGCC TCCATTCTTC AAAAATCTAA TAGCTTCACT TATTCCTAAT 
ATTGCGGATA GAGCACCAAT TGGCAAACAT TTTGGGGATG TCTTGCAGCC AGCAAAACCT 
GAATACAGAG TGGGAGAAGT GGTTGAAGTT ATATTTGTAG GCGCTAACCC AAAGAATTCA 
GCAGAGAACC AGACCCATCA AACCTTCCTC ACTGTGGAGA AATACGAGGA CTCTGTAGCT 
GACTGGCAGA TAATGTATAA CGATGCCTCC TGGGAGACGA GGTTTTATTG GCACAAAGGA 
ATACTGGGTC TGAGCAATGC AACAATATAC TGGCATATTC CAGATACTGC CTACCCTGGA 
ATCTACAGAA TAAGATATTT TGGACACAAT CGGAAGCAGG AACTTCTGAA ACCCGCTGTC 
ATACTAGCAT TTGAAGGAAT TTCTTCTCCT TTTGAAGTTG TCACTACTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Asah2 ( XM_011247290.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011245592.1 Mus musculus N-acylsphingosine amidohydrolase 2 (Asah2), transcript variant X7, mRNA.

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CloneID	OMu10953
Clone ID Related Accession (Same CDS sequence)	XM_011247289.1 , XM_011247284.2 , XM_011247285.2 , XM_011247283.2 , XM_011247286.2 , XM_011247287.2 , XM_011247290.2 , NM_018830.1
Accession Version	XM_011247290.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2271bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	neutral ceramidase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011247290.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAAGC GAACCTTCTC CACCTTGGAG GCATTCCTCA TTTTCCTTCT GGTAATAATG 
ACAGTCATCA CAGTGGCCCT TCTCACCCTC TTGTTTGTTA CCAGTGGGAC CATTGAAAAC 
CACAAAGATT CAGGAAATCA CTGGTTTTCA ACCACTCTGG GCTCCACGAC AACCCAGCCC 
CCTCCAATTA CACAGACTCC AAACTTCCCT TCATTTCGGA ACTTCAGTGG CTACTACATT 
GGCGTTGGGA GAGCAGATTG CACAGGACAA GTGTCAGATA TCAATTTGAT GGGCTATGGC 
AAAAATGGCC AGAATGCACG GGGTCTCCTC ACCAGGCTGT TCAGCCGTGC TTTTATCTTG 
GCGGATCCAG ATGGGTCAAA TCGAATGGCA TTTGTGAGCG TGGAACTATG TATGATTTCC 
CAACGACTGA GGTTGGAGGT CCTGAAGAGA CTAGAGAGTA AATATGGCTC TCTGTATCGA 
AGAGACAATG TTATCCTGAG TGCCATTCAC ACACACTCTG GCCCAGCAGG GTTTTTCCAA 
TATACACTCT ATATACTCGC CAGCGAGGGA TTCAGCAACC GGACCTTTCA GTACATAGTC 
TCTGGGATCA TGAAGAGCAT TGATATAGCT CACACAAATC TTAAACCAGG CAAAATCTTT 
ATCAACAAAG GAAATGTTGC TAATGTGCAG ATCAACCGAA GCCCCTCCTC TTACCTTCTG 
AATCCACAGT CAGAGAGAGC AAGGTATTCT TCAAACACAG ACAAGGAAAT GCTGGTCTTG 
AAACTGGTGG ATTTGAATGG AGAAGACTTG GGTCTTATCA GCTGGTTTGC CATCCACCCC 
GTGAGCATGA ACAATAGCAA CCACTTTGTG AATAGTGACA ATATGGGCTA TGCGGCTTAC 
CTTTTTGAGC AAGAAAAGAA CAAAGGCTAT CTGCCTGGAC AGGGACCGTT TGTAGCAGGC 
TTTGCTTCAT CAAATCTCGG AGACGTGTCA CCCAACATTC TTGGCCCGCA TTGTGTCAAC 
ACAGGGGAGT CTTGTGACAA CGACAAGAGC ACCTGTCCCA ACGGTGGGCC TAGCATGTGC 
ATGGCCAGCG GACCTGGACA AGACATGTTT GAGAGCACAC ACATTATAGG ACGGATCATC 
TATCAGAAGG CCAAGGAGCT GTATGCCTCT GCCTCCCAGG AGGTGACCGG CCCAGTGCTT 
GCAGCTCACC AGTGGGTGAA CATGACAGAT GTGAGCGTCC AGCTCAATGC CACACACACA 
GTGAAGACGT GTAAACCTGC CCTGGGCTAC AGTTTTGCCG CAGGCACAAT TGATGGAGTT 
TCGGGCCTCA ATATTACACA GGGAACTACG GAAGGGGATC CATTCTGGGA CACTCTTCGG 
GACCAGCTCT TGGGAAAACC ATCTGAAGAG ATTGTAGAGT GTCAGAAACC CAAACCAATC 
CTGCTTCACA GTGGAGAGCT GACGATACCA CATCCTTGGC AACCAGATAT TGTTGATGTT 
CAGATTGTTA CCGTTGGGTC CTTGGCCATA GCTGCTATCC CTGGGGAATT AACAACCATG 
TCGGGACGAA GATTTCGTGA GGCAATTAAA AAAGAATTTG CACTTTATGG GATGAAGGAT 
ATGACCGTTG TTATCGCAGG TCTAAGCAAT GTTTATACAC ATTACATTAC CACATATGAA 
GAATACCAGG CTCAGCGGTA CGAGGCAGCA TCTACAATCT ATGGACCACA CACCCTGTCT 
GCATACATCC AACTCTTCAG AGACCTTGCT AAGGCAATTG CTACGGACAC AGTAGCCAAC 
ATGAGCAGTG GTCCCGAGCC TCCATTCTTC AAAAATCTAA TAGCTTCACT TATTCCTAAT 
ATTGCGGATA GAGCACCAAT TGGCAAACAT TTTGGGGATG TCTTGCAGCC AGCAAAACCT 
GAATACAGAG TGGGAGAAGT GGTTGAAGTT ATATTTGTAG GCGCTAACCC AAAGAATTCA 
GCAGAGAACC AGACCCATCA AACCTTCCTC ACTGTGGAGA AATACGAGGA CTCTGTAGCT 
GACTGGCAGA TAATGTATAA CGATGCCTCC TGGGAGACGA GGTTTTATTG GCACAAAGGA 
ATACTGGGTC TGAGCAATGC AACAATATAC TGGCATATTC CAGATACTGC CTACCCTGGA 
ATCTACAGAA TAAGATATTT TGGACACAAT CGGAAGCAGG AACTTCTGAA ACCCGCTGTC 
ATACTAGCAT TTGAAGGAAT TTCTTCTCCT TTTGAAGTTG TCACTACTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011245592.1
CDS	765..3035
Translation	MAKRTFSTLEAFLIFLLVIMTVITVALLTLLFVTSGTIENHKDSGNHWFSTTLGSTTTQPPPITQTPNFPSFRNFSGYYIGVGRADCTGQVSDINLMGYGKNGQNARGLLTRLFSRAFILADPDGSNRMAFVSVELCMISQRLRLEVLKRLESKYGSLYRRDNVILSAIHTHSGPAGFFQYTLYILASEGFSNRTFQYIVSGIMKSIDIAHTNLKPGKIFINKGNVANVQINRSPSSYLLNPQSERARYSSNTDKEMLVLKLVDLNGEDLGLISWFAIHPVSMNNSNHFVNSDNMGYAAYLFEQEKNKGYLPGQGPFVAGFASSNLGDVSPNILGPHCVNTGESCDNDKSTCPNGGPSMCMASGPGQDMFESTHIIGRIIYQKAKELYASASQEVTGPVLAAHQWVNMTDVSVQLNATHTVKTCKPALGYSFAAGTIDGVSGLNITQGTTEGDPFWDTLRDQLLGKPSEEIVECQKPKPILLHSGELTIPHPWQPDIVDVQIVTVGSLAIAAIPGELTTMSGRRFREAIKKEFALYGMKDMTVVIAGLSNVYTHYITTYEEYQAQRYEAASTIYGPHTLSAYIQLFRDLAKAIATDTVANMSSGPEPPFFKNLIASLIPNIADRAPIGKHFGDVLQPAKPEYRVGEVVEVIFVGANPKNSAENQTHQTFLTVEKYEDSVADWQIMYNDASWETRFYWHKGILGLSNATIYWHIPDTAYPGIYRIRYFGHNRKQELLKPAVILAFEGISSPFEVVTT

Target ORF information:

RefSeq Version	XM_011247290.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus N-acylsphingosine amidohydrolase 2 (Asah2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011247290.2

ORF Insert Sequence:

ATGGCAAAGC GAACCTTCTC CACCTTGGAG GCATTCCTCA TTTTCCTTCT GGTAATAATG 
ACAGTCATCA CAGTGGCCCT TCTCACCCTC TTGTTTGTTA CCAGTGGGAC CATTGAAAAC 
CACAAAGATT CAGGAAATCA CTGGTTTTCA ACCACTCTGG GCTCCACGAC AACCCAGCCC 
CCTCCAATTA CACAGACTCC AAACTTCCCT TCATTTCGGA ACTTCAGTGG CTACTACATT 
GGCGTTGGGA GAGCAGATTG CACAGGACAA GTGTCAGATA TCAATTTGAT GGGCTATGGC 
AAAAATGGCC AGAATGCACG GGGTCTCCTC ACCAGGCTGT TCAGCCGTGC TTTTATCTTG 
GCGGATCCAG ATGGGTCAAA TCGAATGGCA TTTGTGAGCG TGGAACTATG TATGATTTCC 
CAACGACTGA GGTTGGAGGT CCTGAAGAGA CTAGAGAGTA AATATGGCTC TCTGTATCGA 
AGAGACAATG TTATCCTGAG TGCCATTCAC ACACACTCTG GCCCAGCAGG GTTTTTCCAA 
TATACACTCT ATATACTCGC CAGCGAGGGA TTCAGCAACC GGACCTTTCA GTACATAGTC 
TCTGGGATCA TGAAGAGCAT TGATATAGCT CACACAAATC TTAAACCAGG CAAAATCTTT 
ATCAACAAAG GAAATGTTGC TAATGTGCAG ATCAACCGAA GCCCCTCCTC TTACCTTCTG 
AATCCACAGT CAGAGAGAGC AAGGTATTCT TCAAACACAG ACAAGGAAAT GCTGGTCTTG 
AAACTGGTGG ATTTGAATGG AGAAGACTTG GGTCTTATCA GCTGGTTTGC CATCCACCCC 
GTGAGCATGA ACAATAGCAA CCACTTTGTG AATAGTGACA ATATGGGCTA TGCGGCTTAC 
CTTTTTGAGC AAGAAAAGAA CAAAGGCTAT CTGCCTGGAC AGGGACCGTT TGTAGCAGGC 
TTTGCTTCAT CAAATCTCGG AGACGTGTCA CCCAACATTC TTGGCCCGCA TTGTGTCAAC 
ACAGGGGAGT CTTGTGACAA CGACAAGAGC ACCTGTCCCA ACGGTGGGCC TAGCATGTGC 
ATGGCCAGCG GACCTGGACA AGACATGTTT GAGAGCACAC ACATTATAGG ACGGATCATC 
TATCAGAAGG CCAAGGAGCT GTATGCCTCT GCCTCCCAGG AGGTGACCGG CCCAGTGCTT 
GCAGCTCACC AGTGGGTGAA CATGACAGAT GTGAGCGTCC AGCTCAATGC CACACACACA 
GTGAAGACGT GTAAACCTGC CCTGGGCTAC AGTTTTGCCG CAGGCACAAT TGATGGAGTT 
TCGGGCCTCA ATATTACACA GGGAACTACG GAAGGGGATC CATTCTGGGA CACTCTTCGG 
GACCAGCTCT TGGGAAAACC ATCTGAAGAG ATTGTAGAGT GTCAGAAACC CAAACCAATC 
CTGCTTCACA GTGGAGAGCT GACGATACCA CATCCTTGGC AACCAGATAT TGTTGATGTT 
CAGATTGTTA CCGTTGGGTC CTTGGCCATA GCTGCTATCC CTGGGGAATT AACAACCATG 
TCGGGACGAA GATTTCGTGA GGCAATTAAA AAAGAATTTG CACTTTATGG GATGAAGGAT 
ATGACCGTTG TTATCGCAGG TCTAAGCAAT GTTTATACAC ATTACATTAC CACATATGAA 
GAATACCAGG CTCAGCGGTA CGAGGCAGCA TCTACAATCT ATGGACCACA CACCCTGTCT 
GCATACATCC AACTCTTCAG AGACCTTGCT AAGGCAATTG CTACGGACAC AGTAGCCAAC 
ATGAGCAGTG GTCCCGAGCC TCCATTCTTC AAAAATCTAA TAGCTTCACT TATTCCTAAT 
ATTGCGGATA GAGCACCAAT TGGCAAACAT TTTGGGGATG TCTTGCAGCC AGCAAAACCT 
GAATACAGAG TGGGAGAAGT GGTTGAAGTT ATATTTGTAG GCGCTAACCC AAAGAATTCA 
GCAGAGAACC AGACCCATCA AACCTTCCTC ACTGTGGAGA AATACGAGGA CTCTGTAGCT 
GACTGGCAGA TAATGTATAA CGATGCCTCC TGGGAGACGA GGTTTTATTG GCACAAAGGA 
ATACTGGGTC TGAGCAATGC AACAATATAC TGGCATATTC CAGATACTGC CTACCCTGGA 
ATCTACAGAA TAAGATATTT TGGACACAAT CGGAAGCAGG AACTTCTGAA ACCCGCTGTC 
ATACTAGCAT TTGAAGGAAT TTCTTCTCCT TTTGAAGTTG TCACTACTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Lactosylceramide contributes to mitochondrial dysfunction in diabetes.
Journal of lipid research57(4)546-62(2016 Apr)
Novgorodov SA,Riley CL,Yu J,Keffler JA,Clarke CJ,Van Laer AO,Baicu CF,Zile MR,Gudz TI

Loss of neutral ceramidase protects cells from nutrient- and energy -deprivation-induced cell death.
The Biochemical journal473(6)743-55(2016 Mar)
Sundaram K,Mather AR,Marimuthu S,Shah PP,Snider AJ,Obeid LM,Hannun YA,Beverly LJ,Siskind LJ

Essential roles of neutral ceramidase and sphingosine in mitochondrial dysfunction due to traumatic brain injury.
The Journal of biological chemistry289(19)13142-54(2014 May)
Novgorodov SA,Riley CL,Yu J,Borg KT,Hannun YA,Proia RL,Kindy MS,Gudz TI

Transcription factor Ctip2 controls epidermal lipid metabolism and regulates expression of genes involved in sphingolipid biosynthesis during skin development.
The Journal of investigative dermatology133(3)668-676(2013 Mar)
Wang Z,Kirkwood JS,Taylor AW,Stevens JF,Leid M,Ganguli-Indra G,Indra AK

Loss of neutral ceramidase increases inflammation in a mouse model of inflammatory bowel disease.
Prostaglandins & other lipid mediators99(3-4)124-30(2012 Dec)
Snider AJ,Wu BX,Jenkins RW,Sticca JA,Kawamori T,Hannun YA,Obeid LM

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Asah2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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