CDase cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CDase
Entrez Gene ID	43618
Full Name	Ceramidase
Synonyms	CG1471,Dmel\CG1471,anon-WO0118547.407,cdase,fcd,slab
General protein information	Preferred Names Ceramidase Names CG1471 gene product from transcript CG1471-RF CDase-PA CDase-PB CDase-PC CDase-PD CDase-PE CDase-PF CG1471-PA CG1471-PB CG1471-PC CG1471-PD CG1471-PE CG1471-PF ceramidase slug-a-bed
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 99F5-99F6

mRNA and Protein(s)

mRNA	Protein	Name
NM_001276180.1	NP_001263109.1	ceramidase, isoform F
NM_143540.2	NP_651797.1	ceramidase, isoform A
NM_170489.2	NP_733368.1	ceramidase, isoform C
NM_170491.2	NP_733370.1	ceramidase, isoform E
NM_170490.2	NP_733369.1	ceramidase, isoform D
NM_170488.2	NP_733367.1	ceramidase, isoform B

Pathways from BioSystems

Homologies

Homo sapiens (human)	ASAH2	NP_063946.2
Drosophila melanogaster (fruit fly)	CDase	NP_733370.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000973	XP_309165.3
Arabidopsis thaliana (thale cress)	AT2G38010	NP_181337.2
Xenopus tropicalis (tropical clawed frog)	asah2	NP_001123830.1
Xenopus tropicalis (tropical clawed frog)	LOC100494440	XP_002935475.2
	ASAH2	XP_001100613.1
Bos taurus (cattle)	ASAH2	XP_002698412.1
Mus musculus (house mouse)	Asah2	NP_061300.1
Rattus norvegicus (Norway rat)	Asah2	NP_446098.1
Danio rerio (zebrafish)	asah2	NP_001007764.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003730	XP_310261.7
Gallus gallus (chicken)	ASAH2	XP_421560.4
Oryza sativa (rice)	Os01g0624000	NP_001043618.1
Pan troglodytes (chimpanzee)	ASAH2	XP_507791.2
Canis lupus familiaris (dog)	ASAH2B	XP_005636694.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

findings show the neutral ceramidase gene CDase encodes the protein that is responsible for all measurable ceramidase activity in Drosophila; studies show strong genetic interaction of Bwa with CDase and the ceramide kinase gene
Title: CDase is a pan-ceramidase in Drosophila.

SLAB ceramidase function controls presynaptic terminal sphingolipid composition to regulate vesicle fusion and trafficking, and thus the strength and reliability of synaptic transmission
Title: Ceramidase regulates synaptic vesicle exocytosis and trafficking.

Data report the molecular cloning and characterization of the Drosophila neutral ceramidase.
Title: Molecular cloning and characterization of a secretory neutral ceramidase of Drosophila melanogaster.

The following CDase gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CDase cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa06655	NM_143540.2 Latest version!	Drosophila melanogaster ceramidase, transcript variant A (CDase), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa06655	NM_170490.2 Latest version!	Drosophila melanogaster ceramidase, transcript variant D (CDase), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa06655	NM_170488.2 Latest version!	Drosophila melanogaster ceramidase, transcript variant B (CDase), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa06655	NM_001276180.1 Latest version!	Drosophila melanogaster ceramidase, transcript variant F (CDase), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa06655	NM_170491.2 Latest version!	Drosophila melanogaster ceramidase, transcript variant E (CDase), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa06655	NM_170489.2 Latest version!	Drosophila melanogaster ceramidase, transcript variant C (CDase), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CDase ( NM_001276180.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001263109.1 Drosophila melanogaster ceramidase, transcript variant F (CDase), mRNA.

Price & Availability↑

CloneID	OFa06655
Clone ID Related Accession (Same CDS sequence)	NM_001276180.1 , NM_143540.2 , NM_170489.2 , NM_170491.2 , NM_170490.2 , NM_170488.2
Accession Version	NM_001276180.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2115bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ceramidase, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTAATA GCAAGATGGC CTTCTTGGCC TTTCTGGCCG TAAGTTTCCT GTGTGGATTG 
GTTAGTGCCA CCTACAAAGT GGGCGTGGGC CGCGCGGATA TCACAGGACC TCCAGTGGAG 
ATCAATTTCA TGGGCTATGC CAACATCAAG CAAGTGGGTC GTGGCATCCA CACCCGTGTC 
TTTGCCCGTG CCTTTGTGGT GGAGGATGAA AAGGGCAACC GAGTGGCCTT CGTGAGCGCA 
GATGCCGGAA TGATGGGCTA CGGATTGAAG AGGGAGGTGA TAAAACGACT CCAGGCACGT 
TATGGCAACA TCTACCACAA TGATAATGTG GCCATCAGTG GCACCCACAC GCACGGTGCT 
CCTGGCGGAT TCCTGATGCA TCTGCTCTAT GACATCTCCA TTCTGGGCTT TGTGCCTCAG 
ACGTTTGAGG TGATGGCTCA GGGACTGTAT CTGTGCATCA AAAGGGCGAC GGACAACCTG 
GTGGATGGTC GCATCTTGCT GTCCAAAACT ACTGTGCTAA ATGTTAACAT CAATCGTTCG 
CCCTCATCCT ACTTGAGGAA TCCCGCCGAG GAGCGTGCCC AATACGAGCA CGATACGGAT 
AAGACACTGA CCCAGCTGCG ATTTGTCGAC CTGGAAAACA ACCTCCTGGG CGCTTTCAAC 
TGGTATGCGG TGCATGCCAC CTCCATGAAC AATACCAACA GACTGGTGAC CAGCGACAAT 
GTGGGTTATG CCGCCCTGCT CCTGGAAAAG GAGTACAATC CGAACAAGAT GCCCGGAAAG 
GGCAAGTTCG TGGGTGCCTT CTGCTCATCC AACCTTGGCG ATGTGTCCCC CAATATAATG 
GGTCCCAAGT GCTCGATCTC CGGCAATGAG TGTGATCTAC TGACCTCCCG TTGTCCCACT 
GGCGAGGGAG ATTGCTTTGC CTCCGGACCC GGTAAGGATA TGTTCGAAAG CACCCAGATC 
TTGGGTCAAC GTCTGGCGGA TGCTGCTCTG GGACTGCTCA ACGAACAGAG CCAGGAGTCC 
ACGGCACGGG AGGTCACTGG AGATGTGAGG TTCATCCACC AGTTTGTGGA CATGCCCAAC 
TACAATGGCA GCACCTACAA TCCGCTGAGC AGGAAGGTCG ACAAGATCAG GGGATGTCAG 
CCGGCCATGG GCTATAGCTT TGCTGCTGGT ACCACCGATG GACCTGGAGC CTTCAGCTTC 
GAGCAGGGAA CCACCACGGA CAACCCCATG TGGAACTTTG TGCGCGACTT CATCGCTGCT 
CCCACGCAGG AGGACATCAA GTGCCATGAG CCCAAGCCCA TTCTTCTGGC CACCGGCAGG 
GCCACCTTTC CCTATGAGTG GCAGCCAAAG ATTGTCTCGG ATCAGCTACT AAAGATCGGC 
GATGTGATCA TAGCCGCCGT GCCCTGCGAG TTCACCACAA TGGCAGGACG TCGTCTGCGA 
AACCAGATTC GAGCAGCTGC CTCCGCCGTC GGAGGCATCG ACACCGAGGT AATCATCGCC 
GGACTGACCA ACATATACAC CAGCTACACG GTGACCCCGG AGGAGTACCA GGCGCAGCGT 
TACGAGGCCG CCTCCACGAT CTTCGGTCCC CATACCCACT CCATTTACAT GGACGTCTTC 
GAACGCCTGA CCAAGGCCAT GATGCGTAAT GAGACCGTGG ATGCGGGTCC AAGTCCGCCG 
TACATGAATG ATGTGATGCT ATCGCTGAAC ACCGGGGTTC TCTTCGACGG ACATCCCATA 
AACACCGACT TTGGCTACGT GAAGTCGCAG CCCAACAAGG AGTACGGCAT CAACGAGACG 
GTGAAGGTCA CCTACATATC CGGAAATCCG CGTAACAACC TCTTCACCGA GAAAACCTAC 
TTCACCATTG AGCGAAAGAT CAACGAGGAT CGCTGGAAGG TGGCCTATAC GGATGCCAGC 
TGGGAGACCA AAATGGTCTG GCACCGAACC AATACGATCC TGGGCTTCAG CGAAATGGAC 
ATCTACTGGG ACATCAGTCC GCAGACTCTT CCCGGGGAAT ATCGCATTCG CCATTCCGGG 
GAGTACAAGT ACATCCTGGG CGGAAAGTAT CCCTACGAGG GACTCACGCA TTCCTTCACC 
GTTAAGGAGG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001263109.1
CDS	47..2161
Translation	MANSKMAFLAFLAVSFLCGLVSATYKVGVGRADITGPPVEINFMGYANIKQVGRGIHTRVFARAFVVEDEKGNRVAFVSADAGMMGYGLKREVIKRLQARYGNIYHNDNVAISGTHTHGAPGGFLMHLLYDISILGFVPQTFEVMAQGLYLCIKRATDNLVDGRILLSKTTVLNVNINRSPSSYLRNPAEERAQYEHDTDKTLTQLRFVDLENNLLGAFNWYAVHATSMNNTNRLVTSDNVGYAALLLEKEYNPNKMPGKGKFVGAFCSSNLGDVSPNIMGPKCSISGNECDLLTSRCPTGEGDCFASGPGKDMFESTQILGQRLADAALGLLNEQSQESTAREVTGDVRFIHQFVDMPNYNGSTYNPLSRKVDKIRGCQPAMGYSFAAGTTDGPGAFSFEQGTTTDNPMWNFVRDFIAAPTQEDIKCHEPKPILLATGRATFPYEWQPKIVSDQLLKIGDVIIAAVPCEFTTMAGRRLRNQIRAAASAVGGIDTEVIIAGLTNIYTSYTVTPEEYQAQRYEAASTIFGPHTHSIYMDVFERLTKAMMRNETVDAGPSPPYMNDVMLSLNTGVLFDGHPINTDFGYVKSQPNKEYGINETVKVTYISGNPRNNLFTEKTYFTIERKINEDRWKVAYTDASWETKMVWHRTNTILGFSEMDIYWDISPQTLPGEYRIRHSGEYKYILGGKYPYEGLTHSFTVKED

Target ORF information:

RefSeq Version	NM_001276180.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ceramidase, transcript variant F (CDase), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001276180.1

ORF Insert Sequence:

ATGGCTAATA GCAAGATGGC CTTCTTGGCC TTTCTGGCCG TAAGTTTCCT GTGTGGATTG 
GTTAGTGCCA CCTACAAAGT GGGCGTGGGC CGCGCGGATA TCACAGGACC TCCAGTGGAG 
ATCAATTTCA TGGGCTATGC CAACATCAAG CAAGTGGGTC GTGGCATCCA CACCCGTGTC 
TTTGCCCGTG CCTTTGTGGT GGAGGATGAA AAGGGCAACC GAGTGGCCTT CGTGAGCGCA 
GATGCCGGAA TGATGGGCTA CGGATTGAAG AGGGAGGTGA TAAAACGACT CCAGGCACGT 
TATGGCAACA TCTACCACAA TGATAATGTG GCCATCAGTG GCACCCACAC GCACGGTGCT 
CCTGGCGGAT TCCTGATGCA TCTGCTCTAT GACATCTCCA TTCTGGGCTT TGTGCCTCAG 
ACGTTTGAGG TGATGGCTCA GGGACTGTAT CTGTGCATCA AAAGGGCGAC GGACAACCTG 
GTGGATGGTC GCATCTTGCT GTCCAAAACT ACTGTGCTAA ATGTTAACAT CAATCGTTCG 
CCCTCATCCT ACTTGAGGAA TCCCGCCGAG GAGCGTGCCC AATACGAGCA CGATACGGAT 
AAGACACTGA CCCAGCTGCG ATTTGTCGAC CTGGAAAACA ACCTCCTGGG CGCTTTCAAC 
TGGTATGCGG TGCATGCCAC CTCCATGAAC AATACCAACA GACTGGTGAC CAGCGACAAT 
GTGGGTTATG CCGCCCTGCT CCTGGAAAAG GAGTACAATC CGAACAAGAT GCCCGGAAAG 
GGCAAGTTCG TGGGTGCCTT CTGCTCATCC AACCTTGGCG ATGTGTCCCC CAATATAATG 
GGTCCCAAGT GCTCGATCTC CGGCAATGAG TGTGATCTAC TGACCTCCCG TTGTCCCACT 
GGCGAGGGAG ATTGCTTTGC CTCCGGACCC GGTAAGGATA TGTTCGAAAG CACCCAGATC 
TTGGGTCAAC GTCTGGCGGA TGCTGCTCTG GGACTGCTCA ACGAACAGAG CCAGGAGTCC 
ACGGCACGGG AGGTCACTGG AGATGTGAGG TTCATCCACC AGTTTGTGGA CATGCCCAAC 
TACAATGGCA GCACCTACAA TCCGCTGAGC AGGAAGGTCG ACAAGATCAG GGGATGTCAG 
CCGGCCATGG GCTATAGCTT TGCTGCTGGT ACCACCGATG GACCTGGAGC CTTCAGCTTC 
GAGCAGGGAA CCACCACGGA CAACCCCATG TGGAACTTTG TGCGCGACTT CATCGCTGCT 
CCCACGCAGG AGGACATCAA GTGCCATGAG CCCAAGCCCA TTCTTCTGGC CACCGGCAGG 
GCCACCTTTC CCTATGAGTG GCAGCCAAAG ATTGTCTCGG ATCAGCTACT AAAGATCGGC 
GATGTGATCA TAGCCGCCGT GCCCTGCGAG TTCACCACAA TGGCAGGACG TCGTCTGCGA 
AACCAGATTC GAGCAGCTGC CTCCGCCGTC GGAGGCATCG ACACCGAGGT AATCATCGCC 
GGACTGACCA ACATATACAC CAGCTACACG GTGACCCCGG AGGAGTACCA GGCGCAGCGT 
TACGAGGCCG CCTCCACGAT CTTCGGTCCC CATACCCACT CCATTTACAT GGACGTCTTC 
GAACGCCTGA CCAAGGCCAT GATGCGTAAT GAGACCGTGG ATGCGGGTCC AAGTCCGCCG 
TACATGAATG ATGTGATGCT ATCGCTGAAC ACCGGGGTTC TCTTCGACGG ACATCCCATA 
AACACCGACT TTGGCTACGT GAAGTCGCAG CCCAACAAGG AGTACGGCAT CAACGAGACG 
GTGAAGGTCA CCTACATATC CGGAAATCCG CGTAACAACC TCTTCACCGA GAAAACCTAC 
TTCACCATTG AGCGAAAGAT CAACGAGGAT CGCTGGAAGG TGGCCTATAC GGATGCCAGC 
TGGGAGACCA AAATGGTCTG GCACCGAACC AATACGATCC TGGGCTTCAG CGAAATGGAC 
ATCTACTGGG ACATCAGTCC GCAGACTCTT CCCGGGGAAT ATCGCATTCG CCATTCCGGG 
GAGTACAAGT ACATCCTGGG CGGAAAGTAT CCCTACGAGG GACTCACGCA TTCCTTCACC 
GTTAAGGAGG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDase ( NM_143540.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_651797.1 Drosophila melanogaster ceramidase, transcript variant A (CDase), mRNA.

Price & Availability↑

CloneID	OFa06655
Clone ID Related Accession (Same CDS sequence)	NM_001276180.1 , NM_143540.2 , NM_170489.2 , NM_170491.2 , NM_170490.2 , NM_170488.2
Accession Version	NM_143540.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2115bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ceramidase, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_143540.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTAATA GCAAGATGGC CTTCTTGGCC TTTCTGGCCG TAAGTTTCCT GTGTGGATTG 
GTTAGTGCCA CCTACAAAGT GGGCGTGGGC CGCGCGGATA TCACAGGACC TCCAGTGGAG 
ATCAATTTCA TGGGCTATGC CAACATCAAG CAAGTGGGTC GTGGCATCCA CACCCGTGTC 
TTTGCCCGTG CCTTTGTGGT GGAGGATGAA AAGGGCAACC GAGTGGCCTT CGTGAGCGCA 
GATGCCGGAA TGATGGGCTA CGGATTGAAG AGGGAGGTGA TAAAACGACT CCAGGCACGT 
TATGGCAACA TCTACCACAA TGATAATGTG GCCATCAGTG GCACCCACAC GCACGGTGCT 
CCTGGCGGAT TCCTGATGCA TCTGCTCTAT GACATCTCCA TTCTGGGCTT TGTGCCTCAG 
ACGTTTGAGG TGATGGCTCA GGGACTGTAT CTGTGCATCA AAAGGGCGAC GGACAACCTG 
GTGGATGGTC GCATCTTGCT GTCCAAAACT ACTGTGCTAA ATGTTAACAT CAATCGTTCG 
CCCTCATCCT ACTTGAGGAA TCCCGCCGAG GAGCGTGCCC AATACGAGCA CGATACGGAT 
AAGACACTGA CCCAGCTGCG ATTTGTCGAC CTGGAAAACA ACCTCCTGGG CGCTTTCAAC 
TGGTATGCGG TGCATGCCAC CTCCATGAAC AATACCAACA GACTGGTGAC CAGCGACAAT 
GTGGGTTATG CCGCCCTGCT CCTGGAAAAG GAGTACAATC CGAACAAGAT GCCCGGAAAG 
GGCAAGTTCG TGGGTGCCTT CTGCTCATCC AACCTTGGCG ATGTGTCCCC CAATATAATG 
GGTCCCAAGT GCTCGATCTC CGGCAATGAG TGTGATCTAC TGACCTCCCG TTGTCCCACT 
GGCGAGGGAG ATTGCTTTGC CTCCGGACCC GGTAAGGATA TGTTCGAAAG CACCCAGATC 
TTGGGTCAAC GTCTGGCGGA TGCTGCTCTG GGACTGCTCA ACGAACAGAG CCAGGAGTCC 
ACGGCACGGG AGGTCACTGG AGATGTGAGG TTCATCCACC AGTTTGTGGA CATGCCCAAC 
TACAATGGCA GCACCTACAA TCCGCTGAGC AGGAAGGTCG ACAAGATCAG GGGATGTCAG 
CCGGCCATGG GCTATAGCTT TGCTGCTGGT ACCACCGATG GACCTGGAGC CTTCAGCTTC 
GAGCAGGGAA CCACCACGGA CAACCCCATG TGGAACTTTG TGCGCGACTT CATCGCTGCT 
CCCACGCAGG AGGACATCAA GTGCCATGAG CCCAAGCCCA TTCTTCTGGC CACCGGCAGG 
GCCACCTTTC CCTATGAGTG GCAGCCAAAG ATTGTCTCGG ATCAGCTACT AAAGATCGGC 
GATGTGATCA TAGCCGCCGT GCCCTGCGAG TTCACCACAA TGGCAGGACG TCGTCTGCGA 
AACCAGATTC GAGCAGCTGC CTCCGCCGTC GGAGGCATCG ACACCGAGGT AATCATCGCC 
GGACTGACCA ACATATACAC CAGCTACACG GTGACCCCGG AGGAGTACCA GGCGCAGCGT 
TACGAGGCCG CCTCCACGAT CTTCGGTCCC CATACCCACT CCATTTACAT GGACGTCTTC 
GAACGCCTGA CCAAGGCCAT GATGCGTAAT GAGACCGTGG ATGCGGGTCC AAGTCCGCCG 
TACATGAATG ATGTGATGCT ATCGCTGAAC ACCGGGGTTC TCTTCGACGG ACATCCCATA 
AACACCGACT TTGGCTACGT GAAGTCGCAG CCCAACAAGG AGTACGGCAT CAACGAGACG 
GTGAAGGTCA CCTACATATC CGGAAATCCG CGTAACAACC TCTTCACCGA GAAAACCTAC 
TTCACCATTG AGCGAAAGAT CAACGAGGAT CGCTGGAAGG TGGCCTATAC GGATGCCAGC 
TGGGAGACCA AAATGGTCTG GCACCGAACC AATACGATCC TGGGCTTCAG CGAAATGGAC 
ATCTACTGGG ACATCAGTCC GCAGACTCTT CCCGGGGAAT ATCGCATTCG CCATTCCGGG 
GAGTACAAGT ACATCCTGGG CGGAAAGTAT CCCTACGAGG GACTCACGCA TTCCTTCACC 
GTTAAGGAGG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_651797.1
CDS	350..2464
Translation	MANSKMAFLAFLAVSFLCGLVSATYKVGVGRADITGPPVEINFMGYANIKQVGRGIHTRVFARAFVVEDEKGNRVAFVSADAGMMGYGLKREVIKRLQARYGNIYHNDNVAISGTHTHGAPGGFLMHLLYDISILGFVPQTFEVMAQGLYLCIKRATDNLVDGRILLSKTTVLNVNINRSPSSYLRNPAEERAQYEHDTDKTLTQLRFVDLENNLLGAFNWYAVHATSMNNTNRLVTSDNVGYAALLLEKEYNPNKMPGKGKFVGAFCSSNLGDVSPNIMGPKCSISGNECDLLTSRCPTGEGDCFASGPGKDMFESTQILGQRLADAALGLLNEQSQESTAREVTGDVRFIHQFVDMPNYNGSTYNPLSRKVDKIRGCQPAMGYSFAAGTTDGPGAFSFEQGTTTDNPMWNFVRDFIAAPTQEDIKCHEPKPILLATGRATFPYEWQPKIVSDQLLKIGDVIIAAVPCEFTTMAGRRLRNQIRAAASAVGGIDTEVIIAGLTNIYTSYTVTPEEYQAQRYEAASTIFGPHTHSIYMDVFERLTKAMMRNETVDAGPSPPYMNDVMLSLNTGVLFDGHPINTDFGYVKSQPNKEYGINETVKVTYISGNPRNNLFTEKTYFTIERKINEDRWKVAYTDASWETKMVWHRTNTILGFSEMDIYWDISPQTLPGEYRIRHSGEYKYILGGKYPYEGLTHSFTVKED

Target ORF information:

RefSeq Version	NM_143540.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ceramidase, transcript variant A (CDase), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_143540.2

ORF Insert Sequence:

ATGGCTAATA GCAAGATGGC CTTCTTGGCC TTTCTGGCCG TAAGTTTCCT GTGTGGATTG 
GTTAGTGCCA CCTACAAAGT GGGCGTGGGC CGCGCGGATA TCACAGGACC TCCAGTGGAG 
ATCAATTTCA TGGGCTATGC CAACATCAAG CAAGTGGGTC GTGGCATCCA CACCCGTGTC 
TTTGCCCGTG CCTTTGTGGT GGAGGATGAA AAGGGCAACC GAGTGGCCTT CGTGAGCGCA 
GATGCCGGAA TGATGGGCTA CGGATTGAAG AGGGAGGTGA TAAAACGACT CCAGGCACGT 
TATGGCAACA TCTACCACAA TGATAATGTG GCCATCAGTG GCACCCACAC GCACGGTGCT 
CCTGGCGGAT TCCTGATGCA TCTGCTCTAT GACATCTCCA TTCTGGGCTT TGTGCCTCAG 
ACGTTTGAGG TGATGGCTCA GGGACTGTAT CTGTGCATCA AAAGGGCGAC GGACAACCTG 
GTGGATGGTC GCATCTTGCT GTCCAAAACT ACTGTGCTAA ATGTTAACAT CAATCGTTCG 
CCCTCATCCT ACTTGAGGAA TCCCGCCGAG GAGCGTGCCC AATACGAGCA CGATACGGAT 
AAGACACTGA CCCAGCTGCG ATTTGTCGAC CTGGAAAACA ACCTCCTGGG CGCTTTCAAC 
TGGTATGCGG TGCATGCCAC CTCCATGAAC AATACCAACA GACTGGTGAC CAGCGACAAT 
GTGGGTTATG CCGCCCTGCT CCTGGAAAAG GAGTACAATC CGAACAAGAT GCCCGGAAAG 
GGCAAGTTCG TGGGTGCCTT CTGCTCATCC AACCTTGGCG ATGTGTCCCC CAATATAATG 
GGTCCCAAGT GCTCGATCTC CGGCAATGAG TGTGATCTAC TGACCTCCCG TTGTCCCACT 
GGCGAGGGAG ATTGCTTTGC CTCCGGACCC GGTAAGGATA TGTTCGAAAG CACCCAGATC 
TTGGGTCAAC GTCTGGCGGA TGCTGCTCTG GGACTGCTCA ACGAACAGAG CCAGGAGTCC 
ACGGCACGGG AGGTCACTGG AGATGTGAGG TTCATCCACC AGTTTGTGGA CATGCCCAAC 
TACAATGGCA GCACCTACAA TCCGCTGAGC AGGAAGGTCG ACAAGATCAG GGGATGTCAG 
CCGGCCATGG GCTATAGCTT TGCTGCTGGT ACCACCGATG GACCTGGAGC CTTCAGCTTC 
GAGCAGGGAA CCACCACGGA CAACCCCATG TGGAACTTTG TGCGCGACTT CATCGCTGCT 
CCCACGCAGG AGGACATCAA GTGCCATGAG CCCAAGCCCA TTCTTCTGGC CACCGGCAGG 
GCCACCTTTC CCTATGAGTG GCAGCCAAAG ATTGTCTCGG ATCAGCTACT AAAGATCGGC 
GATGTGATCA TAGCCGCCGT GCCCTGCGAG TTCACCACAA TGGCAGGACG TCGTCTGCGA 
AACCAGATTC GAGCAGCTGC CTCCGCCGTC GGAGGCATCG ACACCGAGGT AATCATCGCC 
GGACTGACCA ACATATACAC CAGCTACACG GTGACCCCGG AGGAGTACCA GGCGCAGCGT 
TACGAGGCCG CCTCCACGAT CTTCGGTCCC CATACCCACT CCATTTACAT GGACGTCTTC 
GAACGCCTGA CCAAGGCCAT GATGCGTAAT GAGACCGTGG ATGCGGGTCC AAGTCCGCCG 
TACATGAATG ATGTGATGCT ATCGCTGAAC ACCGGGGTTC TCTTCGACGG ACATCCCATA 
AACACCGACT TTGGCTACGT GAAGTCGCAG CCCAACAAGG AGTACGGCAT CAACGAGACG 
GTGAAGGTCA CCTACATATC CGGAAATCCG CGTAACAACC TCTTCACCGA GAAAACCTAC 
TTCACCATTG AGCGAAAGAT CAACGAGGAT CGCTGGAAGG TGGCCTATAC GGATGCCAGC 
TGGGAGACCA AAATGGTCTG GCACCGAACC AATACGATCC TGGGCTTCAG CGAAATGGAC 
ATCTACTGGG ACATCAGTCC GCAGACTCTT CCCGGGGAAT ATCGCATTCG CCATTCCGGG 
GAGTACAAGT ACATCCTGGG CGGAAAGTAT CCCTACGAGG GACTCACGCA TTCCTTCACC 
GTTAAGGAGG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDase ( NM_170489.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_733368.1 Drosophila melanogaster ceramidase, transcript variant C (CDase), mRNA.

Price & Availability↑

CloneID	OFa06655
Clone ID Related Accession (Same CDS sequence)	NM_001276180.1 , NM_143540.2 , NM_170489.2 , NM_170491.2 , NM_170490.2 , NM_170488.2
Accession Version	NM_170489.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2115bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ceramidase, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_170489.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTAATA GCAAGATGGC CTTCTTGGCC TTTCTGGCCG TAAGTTTCCT GTGTGGATTG 
GTTAGTGCCA CCTACAAAGT GGGCGTGGGC CGCGCGGATA TCACAGGACC TCCAGTGGAG 
ATCAATTTCA TGGGCTATGC CAACATCAAG CAAGTGGGTC GTGGCATCCA CACCCGTGTC 
TTTGCCCGTG CCTTTGTGGT GGAGGATGAA AAGGGCAACC GAGTGGCCTT CGTGAGCGCA 
GATGCCGGAA TGATGGGCTA CGGATTGAAG AGGGAGGTGA TAAAACGACT CCAGGCACGT 
TATGGCAACA TCTACCACAA TGATAATGTG GCCATCAGTG GCACCCACAC GCACGGTGCT 
CCTGGCGGAT TCCTGATGCA TCTGCTCTAT GACATCTCCA TTCTGGGCTT TGTGCCTCAG 
ACGTTTGAGG TGATGGCTCA GGGACTGTAT CTGTGCATCA AAAGGGCGAC GGACAACCTG 
GTGGATGGTC GCATCTTGCT GTCCAAAACT ACTGTGCTAA ATGTTAACAT CAATCGTTCG 
CCCTCATCCT ACTTGAGGAA TCCCGCCGAG GAGCGTGCCC AATACGAGCA CGATACGGAT 
AAGACACTGA CCCAGCTGCG ATTTGTCGAC CTGGAAAACA ACCTCCTGGG CGCTTTCAAC 
TGGTATGCGG TGCATGCCAC CTCCATGAAC AATACCAACA GACTGGTGAC CAGCGACAAT 
GTGGGTTATG CCGCCCTGCT CCTGGAAAAG GAGTACAATC CGAACAAGAT GCCCGGAAAG 
GGCAAGTTCG TGGGTGCCTT CTGCTCATCC AACCTTGGCG ATGTGTCCCC CAATATAATG 
GGTCCCAAGT GCTCGATCTC CGGCAATGAG TGTGATCTAC TGACCTCCCG TTGTCCCACT 
GGCGAGGGAG ATTGCTTTGC CTCCGGACCC GGTAAGGATA TGTTCGAAAG CACCCAGATC 
TTGGGTCAAC GTCTGGCGGA TGCTGCTCTG GGACTGCTCA ACGAACAGAG CCAGGAGTCC 
ACGGCACGGG AGGTCACTGG AGATGTGAGG TTCATCCACC AGTTTGTGGA CATGCCCAAC 
TACAATGGCA GCACCTACAA TCCGCTGAGC AGGAAGGTCG ACAAGATCAG GGGATGTCAG 
CCGGCCATGG GCTATAGCTT TGCTGCTGGT ACCACCGATG GACCTGGAGC CTTCAGCTTC 
GAGCAGGGAA CCACCACGGA CAACCCCATG TGGAACTTTG TGCGCGACTT CATCGCTGCT 
CCCACGCAGG AGGACATCAA GTGCCATGAG CCCAAGCCCA TTCTTCTGGC CACCGGCAGG 
GCCACCTTTC CCTATGAGTG GCAGCCAAAG ATTGTCTCGG ATCAGCTACT AAAGATCGGC 
GATGTGATCA TAGCCGCCGT GCCCTGCGAG TTCACCACAA TGGCAGGACG TCGTCTGCGA 
AACCAGATTC GAGCAGCTGC CTCCGCCGTC GGAGGCATCG ACACCGAGGT AATCATCGCC 
GGACTGACCA ACATATACAC CAGCTACACG GTGACCCCGG AGGAGTACCA GGCGCAGCGT 
TACGAGGCCG CCTCCACGAT CTTCGGTCCC CATACCCACT CCATTTACAT GGACGTCTTC 
GAACGCCTGA CCAAGGCCAT GATGCGTAAT GAGACCGTGG ATGCGGGTCC AAGTCCGCCG 
TACATGAATG ATGTGATGCT ATCGCTGAAC ACCGGGGTTC TCTTCGACGG ACATCCCATA 
AACACCGACT TTGGCTACGT GAAGTCGCAG CCCAACAAGG AGTACGGCAT CAACGAGACG 
GTGAAGGTCA CCTACATATC CGGAAATCCG CGTAACAACC TCTTCACCGA GAAAACCTAC 
TTCACCATTG AGCGAAAGAT CAACGAGGAT CGCTGGAAGG TGGCCTATAC GGATGCCAGC 
TGGGAGACCA AAATGGTCTG GCACCGAACC AATACGATCC TGGGCTTCAG CGAAATGGAC 
ATCTACTGGG ACATCAGTCC GCAGACTCTT CCCGGGGAAT ATCGCATTCG CCATTCCGGG 
GAGTACAAGT ACATCCTGGG CGGAAAGTAT CCCTACGAGG GACTCACGCA TTCCTTCACC 
GTTAAGGAGG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_733368.1
CDS	455..2569
Translation	MANSKMAFLAFLAVSFLCGLVSATYKVGVGRADITGPPVEINFMGYANIKQVGRGIHTRVFARAFVVEDEKGNRVAFVSADAGMMGYGLKREVIKRLQARYGNIYHNDNVAISGTHTHGAPGGFLMHLLYDISILGFVPQTFEVMAQGLYLCIKRATDNLVDGRILLSKTTVLNVNINRSPSSYLRNPAEERAQYEHDTDKTLTQLRFVDLENNLLGAFNWYAVHATSMNNTNRLVTSDNVGYAALLLEKEYNPNKMPGKGKFVGAFCSSNLGDVSPNIMGPKCSISGNECDLLTSRCPTGEGDCFASGPGKDMFESTQILGQRLADAALGLLNEQSQESTAREVTGDVRFIHQFVDMPNYNGSTYNPLSRKVDKIRGCQPAMGYSFAAGTTDGPGAFSFEQGTTTDNPMWNFVRDFIAAPTQEDIKCHEPKPILLATGRATFPYEWQPKIVSDQLLKIGDVIIAAVPCEFTTMAGRRLRNQIRAAASAVGGIDTEVIIAGLTNIYTSYTVTPEEYQAQRYEAASTIFGPHTHSIYMDVFERLTKAMMRNETVDAGPSPPYMNDVMLSLNTGVLFDGHPINTDFGYVKSQPNKEYGINETVKVTYISGNPRNNLFTEKTYFTIERKINEDRWKVAYTDASWETKMVWHRTNTILGFSEMDIYWDISPQTLPGEYRIRHSGEYKYILGGKYPYEGLTHSFTVKED

Target ORF information:

RefSeq Version	NM_170489.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ceramidase, transcript variant C (CDase), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170489.2

ORF Insert Sequence:

ATGGCTAATA GCAAGATGGC CTTCTTGGCC TTTCTGGCCG TAAGTTTCCT GTGTGGATTG 
GTTAGTGCCA CCTACAAAGT GGGCGTGGGC CGCGCGGATA TCACAGGACC TCCAGTGGAG 
ATCAATTTCA TGGGCTATGC CAACATCAAG CAAGTGGGTC GTGGCATCCA CACCCGTGTC 
TTTGCCCGTG CCTTTGTGGT GGAGGATGAA AAGGGCAACC GAGTGGCCTT CGTGAGCGCA 
GATGCCGGAA TGATGGGCTA CGGATTGAAG AGGGAGGTGA TAAAACGACT CCAGGCACGT 
TATGGCAACA TCTACCACAA TGATAATGTG GCCATCAGTG GCACCCACAC GCACGGTGCT 
CCTGGCGGAT TCCTGATGCA TCTGCTCTAT GACATCTCCA TTCTGGGCTT TGTGCCTCAG 
ACGTTTGAGG TGATGGCTCA GGGACTGTAT CTGTGCATCA AAAGGGCGAC GGACAACCTG 
GTGGATGGTC GCATCTTGCT GTCCAAAACT ACTGTGCTAA ATGTTAACAT CAATCGTTCG 
CCCTCATCCT ACTTGAGGAA TCCCGCCGAG GAGCGTGCCC AATACGAGCA CGATACGGAT 
AAGACACTGA CCCAGCTGCG ATTTGTCGAC CTGGAAAACA ACCTCCTGGG CGCTTTCAAC 
TGGTATGCGG TGCATGCCAC CTCCATGAAC AATACCAACA GACTGGTGAC CAGCGACAAT 
GTGGGTTATG CCGCCCTGCT CCTGGAAAAG GAGTACAATC CGAACAAGAT GCCCGGAAAG 
GGCAAGTTCG TGGGTGCCTT CTGCTCATCC AACCTTGGCG ATGTGTCCCC CAATATAATG 
GGTCCCAAGT GCTCGATCTC CGGCAATGAG TGTGATCTAC TGACCTCCCG TTGTCCCACT 
GGCGAGGGAG ATTGCTTTGC CTCCGGACCC GGTAAGGATA TGTTCGAAAG CACCCAGATC 
TTGGGTCAAC GTCTGGCGGA TGCTGCTCTG GGACTGCTCA ACGAACAGAG CCAGGAGTCC 
ACGGCACGGG AGGTCACTGG AGATGTGAGG TTCATCCACC AGTTTGTGGA CATGCCCAAC 
TACAATGGCA GCACCTACAA TCCGCTGAGC AGGAAGGTCG ACAAGATCAG GGGATGTCAG 
CCGGCCATGG GCTATAGCTT TGCTGCTGGT ACCACCGATG GACCTGGAGC CTTCAGCTTC 
GAGCAGGGAA CCACCACGGA CAACCCCATG TGGAACTTTG TGCGCGACTT CATCGCTGCT 
CCCACGCAGG AGGACATCAA GTGCCATGAG CCCAAGCCCA TTCTTCTGGC CACCGGCAGG 
GCCACCTTTC CCTATGAGTG GCAGCCAAAG ATTGTCTCGG ATCAGCTACT AAAGATCGGC 
GATGTGATCA TAGCCGCCGT GCCCTGCGAG TTCACCACAA TGGCAGGACG TCGTCTGCGA 
AACCAGATTC GAGCAGCTGC CTCCGCCGTC GGAGGCATCG ACACCGAGGT AATCATCGCC 
GGACTGACCA ACATATACAC CAGCTACACG GTGACCCCGG AGGAGTACCA GGCGCAGCGT 
TACGAGGCCG CCTCCACGAT CTTCGGTCCC CATACCCACT CCATTTACAT GGACGTCTTC 
GAACGCCTGA CCAAGGCCAT GATGCGTAAT GAGACCGTGG ATGCGGGTCC AAGTCCGCCG 
TACATGAATG ATGTGATGCT ATCGCTGAAC ACCGGGGTTC TCTTCGACGG ACATCCCATA 
AACACCGACT TTGGCTACGT GAAGTCGCAG CCCAACAAGG AGTACGGCAT CAACGAGACG 
GTGAAGGTCA CCTACATATC CGGAAATCCG CGTAACAACC TCTTCACCGA GAAAACCTAC 
TTCACCATTG AGCGAAAGAT CAACGAGGAT CGCTGGAAGG TGGCCTATAC GGATGCCAGC 
TGGGAGACCA AAATGGTCTG GCACCGAACC AATACGATCC TGGGCTTCAG CGAAATGGAC 
ATCTACTGGG ACATCAGTCC GCAGACTCTT CCCGGGGAAT ATCGCATTCG CCATTCCGGG 
GAGTACAAGT ACATCCTGGG CGGAAAGTAT CCCTACGAGG GACTCACGCA TTCCTTCACC 
GTTAAGGAGG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDase ( NM_170491.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_733370.1 Drosophila melanogaster ceramidase, transcript variant E (CDase), mRNA.

Price & Availability↑

CloneID	OFa06655
Clone ID Related Accession (Same CDS sequence)	NM_001276180.1 , NM_143540.2 , NM_170489.2 , NM_170491.2 , NM_170490.2 , NM_170488.2
Accession Version	NM_170491.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2115bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ceramidase, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_170491.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTAATA GCAAGATGGC CTTCTTGGCC TTTCTGGCCG TAAGTTTCCT GTGTGGATTG 
GTTAGTGCCA CCTACAAAGT GGGCGTGGGC CGCGCGGATA TCACAGGACC TCCAGTGGAG 
ATCAATTTCA TGGGCTATGC CAACATCAAG CAAGTGGGTC GTGGCATCCA CACCCGTGTC 
TTTGCCCGTG CCTTTGTGGT GGAGGATGAA AAGGGCAACC GAGTGGCCTT CGTGAGCGCA 
GATGCCGGAA TGATGGGCTA CGGATTGAAG AGGGAGGTGA TAAAACGACT CCAGGCACGT 
TATGGCAACA TCTACCACAA TGATAATGTG GCCATCAGTG GCACCCACAC GCACGGTGCT 
CCTGGCGGAT TCCTGATGCA TCTGCTCTAT GACATCTCCA TTCTGGGCTT TGTGCCTCAG 
ACGTTTGAGG TGATGGCTCA GGGACTGTAT CTGTGCATCA AAAGGGCGAC GGACAACCTG 
GTGGATGGTC GCATCTTGCT GTCCAAAACT ACTGTGCTAA ATGTTAACAT CAATCGTTCG 
CCCTCATCCT ACTTGAGGAA TCCCGCCGAG GAGCGTGCCC AATACGAGCA CGATACGGAT 
AAGACACTGA CCCAGCTGCG ATTTGTCGAC CTGGAAAACA ACCTCCTGGG CGCTTTCAAC 
TGGTATGCGG TGCATGCCAC CTCCATGAAC AATACCAACA GACTGGTGAC CAGCGACAAT 
GTGGGTTATG CCGCCCTGCT CCTGGAAAAG GAGTACAATC CGAACAAGAT GCCCGGAAAG 
GGCAAGTTCG TGGGTGCCTT CTGCTCATCC AACCTTGGCG ATGTGTCCCC CAATATAATG 
GGTCCCAAGT GCTCGATCTC CGGCAATGAG TGTGATCTAC TGACCTCCCG TTGTCCCACT 
GGCGAGGGAG ATTGCTTTGC CTCCGGACCC GGTAAGGATA TGTTCGAAAG CACCCAGATC 
TTGGGTCAAC GTCTGGCGGA TGCTGCTCTG GGACTGCTCA ACGAACAGAG CCAGGAGTCC 
ACGGCACGGG AGGTCACTGG AGATGTGAGG TTCATCCACC AGTTTGTGGA CATGCCCAAC 
TACAATGGCA GCACCTACAA TCCGCTGAGC AGGAAGGTCG ACAAGATCAG GGGATGTCAG 
CCGGCCATGG GCTATAGCTT TGCTGCTGGT ACCACCGATG GACCTGGAGC CTTCAGCTTC 
GAGCAGGGAA CCACCACGGA CAACCCCATG TGGAACTTTG TGCGCGACTT CATCGCTGCT 
CCCACGCAGG AGGACATCAA GTGCCATGAG CCCAAGCCCA TTCTTCTGGC CACCGGCAGG 
GCCACCTTTC CCTATGAGTG GCAGCCAAAG ATTGTCTCGG ATCAGCTACT AAAGATCGGC 
GATGTGATCA TAGCCGCCGT GCCCTGCGAG TTCACCACAA TGGCAGGACG TCGTCTGCGA 
AACCAGATTC GAGCAGCTGC CTCCGCCGTC GGAGGCATCG ACACCGAGGT AATCATCGCC 
GGACTGACCA ACATATACAC CAGCTACACG GTGACCCCGG AGGAGTACCA GGCGCAGCGT 
TACGAGGCCG CCTCCACGAT CTTCGGTCCC CATACCCACT CCATTTACAT GGACGTCTTC 
GAACGCCTGA CCAAGGCCAT GATGCGTAAT GAGACCGTGG ATGCGGGTCC AAGTCCGCCG 
TACATGAATG ATGTGATGCT ATCGCTGAAC ACCGGGGTTC TCTTCGACGG ACATCCCATA 
AACACCGACT TTGGCTACGT GAAGTCGCAG CCCAACAAGG AGTACGGCAT CAACGAGACG 
GTGAAGGTCA CCTACATATC CGGAAATCCG CGTAACAACC TCTTCACCGA GAAAACCTAC 
TTCACCATTG AGCGAAAGAT CAACGAGGAT CGCTGGAAGG TGGCCTATAC GGATGCCAGC 
TGGGAGACCA AAATGGTCTG GCACCGAACC AATACGATCC TGGGCTTCAG CGAAATGGAC 
ATCTACTGGG ACATCAGTCC GCAGACTCTT CCCGGGGAAT ATCGCATTCG CCATTCCGGG 
GAGTACAAGT ACATCCTGGG CGGAAAGTAT CCCTACGAGG GACTCACGCA TTCCTTCACC 
GTTAAGGAGG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_733370.1
CDS	586..2700
Translation	MANSKMAFLAFLAVSFLCGLVSATYKVGVGRADITGPPVEINFMGYANIKQVGRGIHTRVFARAFVVEDEKGNRVAFVSADAGMMGYGLKREVIKRLQARYGNIYHNDNVAISGTHTHGAPGGFLMHLLYDISILGFVPQTFEVMAQGLYLCIKRATDNLVDGRILLSKTTVLNVNINRSPSSYLRNPAEERAQYEHDTDKTLTQLRFVDLENNLLGAFNWYAVHATSMNNTNRLVTSDNVGYAALLLEKEYNPNKMPGKGKFVGAFCSSNLGDVSPNIMGPKCSISGNECDLLTSRCPTGEGDCFASGPGKDMFESTQILGQRLADAALGLLNEQSQESTAREVTGDVRFIHQFVDMPNYNGSTYNPLSRKVDKIRGCQPAMGYSFAAGTTDGPGAFSFEQGTTTDNPMWNFVRDFIAAPTQEDIKCHEPKPILLATGRATFPYEWQPKIVSDQLLKIGDVIIAAVPCEFTTMAGRRLRNQIRAAASAVGGIDTEVIIAGLTNIYTSYTVTPEEYQAQRYEAASTIFGPHTHSIYMDVFERLTKAMMRNETVDAGPSPPYMNDVMLSLNTGVLFDGHPINTDFGYVKSQPNKEYGINETVKVTYISGNPRNNLFTEKTYFTIERKINEDRWKVAYTDASWETKMVWHRTNTILGFSEMDIYWDISPQTLPGEYRIRHSGEYKYILGGKYPYEGLTHSFTVKED

Target ORF information:

RefSeq Version	NM_170491.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ceramidase, transcript variant E (CDase), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170491.2

ORF Insert Sequence:

ATGGCTAATA GCAAGATGGC CTTCTTGGCC TTTCTGGCCG TAAGTTTCCT GTGTGGATTG 
GTTAGTGCCA CCTACAAAGT GGGCGTGGGC CGCGCGGATA TCACAGGACC TCCAGTGGAG 
ATCAATTTCA TGGGCTATGC CAACATCAAG CAAGTGGGTC GTGGCATCCA CACCCGTGTC 
TTTGCCCGTG CCTTTGTGGT GGAGGATGAA AAGGGCAACC GAGTGGCCTT CGTGAGCGCA 
GATGCCGGAA TGATGGGCTA CGGATTGAAG AGGGAGGTGA TAAAACGACT CCAGGCACGT 
TATGGCAACA TCTACCACAA TGATAATGTG GCCATCAGTG GCACCCACAC GCACGGTGCT 
CCTGGCGGAT TCCTGATGCA TCTGCTCTAT GACATCTCCA TTCTGGGCTT TGTGCCTCAG 
ACGTTTGAGG TGATGGCTCA GGGACTGTAT CTGTGCATCA AAAGGGCGAC GGACAACCTG 
GTGGATGGTC GCATCTTGCT GTCCAAAACT ACTGTGCTAA ATGTTAACAT CAATCGTTCG 
CCCTCATCCT ACTTGAGGAA TCCCGCCGAG GAGCGTGCCC AATACGAGCA CGATACGGAT 
AAGACACTGA CCCAGCTGCG ATTTGTCGAC CTGGAAAACA ACCTCCTGGG CGCTTTCAAC 
TGGTATGCGG TGCATGCCAC CTCCATGAAC AATACCAACA GACTGGTGAC CAGCGACAAT 
GTGGGTTATG CCGCCCTGCT CCTGGAAAAG GAGTACAATC CGAACAAGAT GCCCGGAAAG 
GGCAAGTTCG TGGGTGCCTT CTGCTCATCC AACCTTGGCG ATGTGTCCCC CAATATAATG 
GGTCCCAAGT GCTCGATCTC CGGCAATGAG TGTGATCTAC TGACCTCCCG TTGTCCCACT 
GGCGAGGGAG ATTGCTTTGC CTCCGGACCC GGTAAGGATA TGTTCGAAAG CACCCAGATC 
TTGGGTCAAC GTCTGGCGGA TGCTGCTCTG GGACTGCTCA ACGAACAGAG CCAGGAGTCC 
ACGGCACGGG AGGTCACTGG AGATGTGAGG TTCATCCACC AGTTTGTGGA CATGCCCAAC 
TACAATGGCA GCACCTACAA TCCGCTGAGC AGGAAGGTCG ACAAGATCAG GGGATGTCAG 
CCGGCCATGG GCTATAGCTT TGCTGCTGGT ACCACCGATG GACCTGGAGC CTTCAGCTTC 
GAGCAGGGAA CCACCACGGA CAACCCCATG TGGAACTTTG TGCGCGACTT CATCGCTGCT 
CCCACGCAGG AGGACATCAA GTGCCATGAG CCCAAGCCCA TTCTTCTGGC CACCGGCAGG 
GCCACCTTTC CCTATGAGTG GCAGCCAAAG ATTGTCTCGG ATCAGCTACT AAAGATCGGC 
GATGTGATCA TAGCCGCCGT GCCCTGCGAG TTCACCACAA TGGCAGGACG TCGTCTGCGA 
AACCAGATTC GAGCAGCTGC CTCCGCCGTC GGAGGCATCG ACACCGAGGT AATCATCGCC 
GGACTGACCA ACATATACAC CAGCTACACG GTGACCCCGG AGGAGTACCA GGCGCAGCGT 
TACGAGGCCG CCTCCACGAT CTTCGGTCCC CATACCCACT CCATTTACAT GGACGTCTTC 
GAACGCCTGA CCAAGGCCAT GATGCGTAAT GAGACCGTGG ATGCGGGTCC AAGTCCGCCG 
TACATGAATG ATGTGATGCT ATCGCTGAAC ACCGGGGTTC TCTTCGACGG ACATCCCATA 
AACACCGACT TTGGCTACGT GAAGTCGCAG CCCAACAAGG AGTACGGCAT CAACGAGACG 
GTGAAGGTCA CCTACATATC CGGAAATCCG CGTAACAACC TCTTCACCGA GAAAACCTAC 
TTCACCATTG AGCGAAAGAT CAACGAGGAT CGCTGGAAGG TGGCCTATAC GGATGCCAGC 
TGGGAGACCA AAATGGTCTG GCACCGAACC AATACGATCC TGGGCTTCAG CGAAATGGAC 
ATCTACTGGG ACATCAGTCC GCAGACTCTT CCCGGGGAAT ATCGCATTCG CCATTCCGGG 
GAGTACAAGT ACATCCTGGG CGGAAAGTAT CCCTACGAGG GACTCACGCA TTCCTTCACC 
GTTAAGGAGG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDase ( NM_170490.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_733369.1 Drosophila melanogaster ceramidase, transcript variant D (CDase), mRNA.

Price & Availability↑

CloneID	OFa06655
Clone ID Related Accession (Same CDS sequence)	NM_001276180.1 , NM_143540.2 , NM_170489.2 , NM_170491.2 , NM_170490.2 , NM_170488.2
Accession Version	NM_170490.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2115bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ceramidase, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_170490.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTAATA GCAAGATGGC CTTCTTGGCC TTTCTGGCCG TAAGTTTCCT GTGTGGATTG 
GTTAGTGCCA CCTACAAAGT GGGCGTGGGC CGCGCGGATA TCACAGGACC TCCAGTGGAG 
ATCAATTTCA TGGGCTATGC CAACATCAAG CAAGTGGGTC GTGGCATCCA CACCCGTGTC 
TTTGCCCGTG CCTTTGTGGT GGAGGATGAA AAGGGCAACC GAGTGGCCTT CGTGAGCGCA 
GATGCCGGAA TGATGGGCTA CGGATTGAAG AGGGAGGTGA TAAAACGACT CCAGGCACGT 
TATGGCAACA TCTACCACAA TGATAATGTG GCCATCAGTG GCACCCACAC GCACGGTGCT 
CCTGGCGGAT TCCTGATGCA TCTGCTCTAT GACATCTCCA TTCTGGGCTT TGTGCCTCAG 
ACGTTTGAGG TGATGGCTCA GGGACTGTAT CTGTGCATCA AAAGGGCGAC GGACAACCTG 
GTGGATGGTC GCATCTTGCT GTCCAAAACT ACTGTGCTAA ATGTTAACAT CAATCGTTCG 
CCCTCATCCT ACTTGAGGAA TCCCGCCGAG GAGCGTGCCC AATACGAGCA CGATACGGAT 
AAGACACTGA CCCAGCTGCG ATTTGTCGAC CTGGAAAACA ACCTCCTGGG CGCTTTCAAC 
TGGTATGCGG TGCATGCCAC CTCCATGAAC AATACCAACA GACTGGTGAC CAGCGACAAT 
GTGGGTTATG CCGCCCTGCT CCTGGAAAAG GAGTACAATC CGAACAAGAT GCCCGGAAAG 
GGCAAGTTCG TGGGTGCCTT CTGCTCATCC AACCTTGGCG ATGTGTCCCC CAATATAATG 
GGTCCCAAGT GCTCGATCTC CGGCAATGAG TGTGATCTAC TGACCTCCCG TTGTCCCACT 
GGCGAGGGAG ATTGCTTTGC CTCCGGACCC GGTAAGGATA TGTTCGAAAG CACCCAGATC 
TTGGGTCAAC GTCTGGCGGA TGCTGCTCTG GGACTGCTCA ACGAACAGAG CCAGGAGTCC 
ACGGCACGGG AGGTCACTGG AGATGTGAGG TTCATCCACC AGTTTGTGGA CATGCCCAAC 
TACAATGGCA GCACCTACAA TCCGCTGAGC AGGAAGGTCG ACAAGATCAG GGGATGTCAG 
CCGGCCATGG GCTATAGCTT TGCTGCTGGT ACCACCGATG GACCTGGAGC CTTCAGCTTC 
GAGCAGGGAA CCACCACGGA CAACCCCATG TGGAACTTTG TGCGCGACTT CATCGCTGCT 
CCCACGCAGG AGGACATCAA GTGCCATGAG CCCAAGCCCA TTCTTCTGGC CACCGGCAGG 
GCCACCTTTC CCTATGAGTG GCAGCCAAAG ATTGTCTCGG ATCAGCTACT AAAGATCGGC 
GATGTGATCA TAGCCGCCGT GCCCTGCGAG TTCACCACAA TGGCAGGACG TCGTCTGCGA 
AACCAGATTC GAGCAGCTGC CTCCGCCGTC GGAGGCATCG ACACCGAGGT AATCATCGCC 
GGACTGACCA ACATATACAC CAGCTACACG GTGACCCCGG AGGAGTACCA GGCGCAGCGT 
TACGAGGCCG CCTCCACGAT CTTCGGTCCC CATACCCACT CCATTTACAT GGACGTCTTC 
GAACGCCTGA CCAAGGCCAT GATGCGTAAT GAGACCGTGG ATGCGGGTCC AAGTCCGCCG 
TACATGAATG ATGTGATGCT ATCGCTGAAC ACCGGGGTTC TCTTCGACGG ACATCCCATA 
AACACCGACT TTGGCTACGT GAAGTCGCAG CCCAACAAGG AGTACGGCAT CAACGAGACG 
GTGAAGGTCA CCTACATATC CGGAAATCCG CGTAACAACC TCTTCACCGA GAAAACCTAC 
TTCACCATTG AGCGAAAGAT CAACGAGGAT CGCTGGAAGG TGGCCTATAC GGATGCCAGC 
TGGGAGACCA AAATGGTCTG GCACCGAACC AATACGATCC TGGGCTTCAG CGAAATGGAC 
ATCTACTGGG ACATCAGTCC GCAGACTCTT CCCGGGGAAT ATCGCATTCG CCATTCCGGG 
GAGTACAAGT ACATCCTGGG CGGAAAGTAT CCCTACGAGG GACTCACGCA TTCCTTCACC 
GTTAAGGAGG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_733369.1
CDS	451..2565
Translation	MANSKMAFLAFLAVSFLCGLVSATYKVGVGRADITGPPVEINFMGYANIKQVGRGIHTRVFARAFVVEDEKGNRVAFVSADAGMMGYGLKREVIKRLQARYGNIYHNDNVAISGTHTHGAPGGFLMHLLYDISILGFVPQTFEVMAQGLYLCIKRATDNLVDGRILLSKTTVLNVNINRSPSSYLRNPAEERAQYEHDTDKTLTQLRFVDLENNLLGAFNWYAVHATSMNNTNRLVTSDNVGYAALLLEKEYNPNKMPGKGKFVGAFCSSNLGDVSPNIMGPKCSISGNECDLLTSRCPTGEGDCFASGPGKDMFESTQILGQRLADAALGLLNEQSQESTAREVTGDVRFIHQFVDMPNYNGSTYNPLSRKVDKIRGCQPAMGYSFAAGTTDGPGAFSFEQGTTTDNPMWNFVRDFIAAPTQEDIKCHEPKPILLATGRATFPYEWQPKIVSDQLLKIGDVIIAAVPCEFTTMAGRRLRNQIRAAASAVGGIDTEVIIAGLTNIYTSYTVTPEEYQAQRYEAASTIFGPHTHSIYMDVFERLTKAMMRNETVDAGPSPPYMNDVMLSLNTGVLFDGHPINTDFGYVKSQPNKEYGINETVKVTYISGNPRNNLFTEKTYFTIERKINEDRWKVAYTDASWETKMVWHRTNTILGFSEMDIYWDISPQTLPGEYRIRHSGEYKYILGGKYPYEGLTHSFTVKED

Target ORF information:

RefSeq Version	NM_170490.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ceramidase, transcript variant D (CDase), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170490.2

ORF Insert Sequence:

ATGGCTAATA GCAAGATGGC CTTCTTGGCC TTTCTGGCCG TAAGTTTCCT GTGTGGATTG 
GTTAGTGCCA CCTACAAAGT GGGCGTGGGC CGCGCGGATA TCACAGGACC TCCAGTGGAG 
ATCAATTTCA TGGGCTATGC CAACATCAAG CAAGTGGGTC GTGGCATCCA CACCCGTGTC 
TTTGCCCGTG CCTTTGTGGT GGAGGATGAA AAGGGCAACC GAGTGGCCTT CGTGAGCGCA 
GATGCCGGAA TGATGGGCTA CGGATTGAAG AGGGAGGTGA TAAAACGACT CCAGGCACGT 
TATGGCAACA TCTACCACAA TGATAATGTG GCCATCAGTG GCACCCACAC GCACGGTGCT 
CCTGGCGGAT TCCTGATGCA TCTGCTCTAT GACATCTCCA TTCTGGGCTT TGTGCCTCAG 
ACGTTTGAGG TGATGGCTCA GGGACTGTAT CTGTGCATCA AAAGGGCGAC GGACAACCTG 
GTGGATGGTC GCATCTTGCT GTCCAAAACT ACTGTGCTAA ATGTTAACAT CAATCGTTCG 
CCCTCATCCT ACTTGAGGAA TCCCGCCGAG GAGCGTGCCC AATACGAGCA CGATACGGAT 
AAGACACTGA CCCAGCTGCG ATTTGTCGAC CTGGAAAACA ACCTCCTGGG CGCTTTCAAC 
TGGTATGCGG TGCATGCCAC CTCCATGAAC AATACCAACA GACTGGTGAC CAGCGACAAT 
GTGGGTTATG CCGCCCTGCT CCTGGAAAAG GAGTACAATC CGAACAAGAT GCCCGGAAAG 
GGCAAGTTCG TGGGTGCCTT CTGCTCATCC AACCTTGGCG ATGTGTCCCC CAATATAATG 
GGTCCCAAGT GCTCGATCTC CGGCAATGAG TGTGATCTAC TGACCTCCCG TTGTCCCACT 
GGCGAGGGAG ATTGCTTTGC CTCCGGACCC GGTAAGGATA TGTTCGAAAG CACCCAGATC 
TTGGGTCAAC GTCTGGCGGA TGCTGCTCTG GGACTGCTCA ACGAACAGAG CCAGGAGTCC 
ACGGCACGGG AGGTCACTGG AGATGTGAGG TTCATCCACC AGTTTGTGGA CATGCCCAAC 
TACAATGGCA GCACCTACAA TCCGCTGAGC AGGAAGGTCG ACAAGATCAG GGGATGTCAG 
CCGGCCATGG GCTATAGCTT TGCTGCTGGT ACCACCGATG GACCTGGAGC CTTCAGCTTC 
GAGCAGGGAA CCACCACGGA CAACCCCATG TGGAACTTTG TGCGCGACTT CATCGCTGCT 
CCCACGCAGG AGGACATCAA GTGCCATGAG CCCAAGCCCA TTCTTCTGGC CACCGGCAGG 
GCCACCTTTC CCTATGAGTG GCAGCCAAAG ATTGTCTCGG ATCAGCTACT AAAGATCGGC 
GATGTGATCA TAGCCGCCGT GCCCTGCGAG TTCACCACAA TGGCAGGACG TCGTCTGCGA 
AACCAGATTC GAGCAGCTGC CTCCGCCGTC GGAGGCATCG ACACCGAGGT AATCATCGCC 
GGACTGACCA ACATATACAC CAGCTACACG GTGACCCCGG AGGAGTACCA GGCGCAGCGT 
TACGAGGCCG CCTCCACGAT CTTCGGTCCC CATACCCACT CCATTTACAT GGACGTCTTC 
GAACGCCTGA CCAAGGCCAT GATGCGTAAT GAGACCGTGG ATGCGGGTCC AAGTCCGCCG 
TACATGAATG ATGTGATGCT ATCGCTGAAC ACCGGGGTTC TCTTCGACGG ACATCCCATA 
AACACCGACT TTGGCTACGT GAAGTCGCAG CCCAACAAGG AGTACGGCAT CAACGAGACG 
GTGAAGGTCA CCTACATATC CGGAAATCCG CGTAACAACC TCTTCACCGA GAAAACCTAC 
TTCACCATTG AGCGAAAGAT CAACGAGGAT CGCTGGAAGG TGGCCTATAC GGATGCCAGC 
TGGGAGACCA AAATGGTCTG GCACCGAACC AATACGATCC TGGGCTTCAG CGAAATGGAC 
ATCTACTGGG ACATCAGTCC GCAGACTCTT CCCGGGGAAT ATCGCATTCG CCATTCCGGG 
GAGTACAAGT ACATCCTGGG CGGAAAGTAT CCCTACGAGG GACTCACGCA TTCCTTCACC 
GTTAAGGAGG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDase ( NM_170488.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_733367.1 Drosophila melanogaster ceramidase, transcript variant B (CDase), mRNA.

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CloneID	OFa06655
Clone ID Related Accession (Same CDS sequence)	NM_001276180.1 , NM_143540.2 , NM_170489.2 , NM_170491.2 , NM_170490.2 , NM_170488.2
Accession Version	NM_170488.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2115bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ceramidase, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_170488.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTAATA GCAAGATGGC CTTCTTGGCC TTTCTGGCCG TAAGTTTCCT GTGTGGATTG 
GTTAGTGCCA CCTACAAAGT GGGCGTGGGC CGCGCGGATA TCACAGGACC TCCAGTGGAG 
ATCAATTTCA TGGGCTATGC CAACATCAAG CAAGTGGGTC GTGGCATCCA CACCCGTGTC 
TTTGCCCGTG CCTTTGTGGT GGAGGATGAA AAGGGCAACC GAGTGGCCTT CGTGAGCGCA 
GATGCCGGAA TGATGGGCTA CGGATTGAAG AGGGAGGTGA TAAAACGACT CCAGGCACGT 
TATGGCAACA TCTACCACAA TGATAATGTG GCCATCAGTG GCACCCACAC GCACGGTGCT 
CCTGGCGGAT TCCTGATGCA TCTGCTCTAT GACATCTCCA TTCTGGGCTT TGTGCCTCAG 
ACGTTTGAGG TGATGGCTCA GGGACTGTAT CTGTGCATCA AAAGGGCGAC GGACAACCTG 
GTGGATGGTC GCATCTTGCT GTCCAAAACT ACTGTGCTAA ATGTTAACAT CAATCGTTCG 
CCCTCATCCT ACTTGAGGAA TCCCGCCGAG GAGCGTGCCC AATACGAGCA CGATACGGAT 
AAGACACTGA CCCAGCTGCG ATTTGTCGAC CTGGAAAACA ACCTCCTGGG CGCTTTCAAC 
TGGTATGCGG TGCATGCCAC CTCCATGAAC AATACCAACA GACTGGTGAC CAGCGACAAT 
GTGGGTTATG CCGCCCTGCT CCTGGAAAAG GAGTACAATC CGAACAAGAT GCCCGGAAAG 
GGCAAGTTCG TGGGTGCCTT CTGCTCATCC AACCTTGGCG ATGTGTCCCC CAATATAATG 
GGTCCCAAGT GCTCGATCTC CGGCAATGAG TGTGATCTAC TGACCTCCCG TTGTCCCACT 
GGCGAGGGAG ATTGCTTTGC CTCCGGACCC GGTAAGGATA TGTTCGAAAG CACCCAGATC 
TTGGGTCAAC GTCTGGCGGA TGCTGCTCTG GGACTGCTCA ACGAACAGAG CCAGGAGTCC 
ACGGCACGGG AGGTCACTGG AGATGTGAGG TTCATCCACC AGTTTGTGGA CATGCCCAAC 
TACAATGGCA GCACCTACAA TCCGCTGAGC AGGAAGGTCG ACAAGATCAG GGGATGTCAG 
CCGGCCATGG GCTATAGCTT TGCTGCTGGT ACCACCGATG GACCTGGAGC CTTCAGCTTC 
GAGCAGGGAA CCACCACGGA CAACCCCATG TGGAACTTTG TGCGCGACTT CATCGCTGCT 
CCCACGCAGG AGGACATCAA GTGCCATGAG CCCAAGCCCA TTCTTCTGGC CACCGGCAGG 
GCCACCTTTC CCTATGAGTG GCAGCCAAAG ATTGTCTCGG ATCAGCTACT AAAGATCGGC 
GATGTGATCA TAGCCGCCGT GCCCTGCGAG TTCACCACAA TGGCAGGACG TCGTCTGCGA 
AACCAGATTC GAGCAGCTGC CTCCGCCGTC GGAGGCATCG ACACCGAGGT AATCATCGCC 
GGACTGACCA ACATATACAC CAGCTACACG GTGACCCCGG AGGAGTACCA GGCGCAGCGT 
TACGAGGCCG CCTCCACGAT CTTCGGTCCC CATACCCACT CCATTTACAT GGACGTCTTC 
GAACGCCTGA CCAAGGCCAT GATGCGTAAT GAGACCGTGG ATGCGGGTCC AAGTCCGCCG 
TACATGAATG ATGTGATGCT ATCGCTGAAC ACCGGGGTTC TCTTCGACGG ACATCCCATA 
AACACCGACT TTGGCTACGT GAAGTCGCAG CCCAACAAGG AGTACGGCAT CAACGAGACG 
GTGAAGGTCA CCTACATATC CGGAAATCCG CGTAACAACC TCTTCACCGA GAAAACCTAC 
TTCACCATTG AGCGAAAGAT CAACGAGGAT CGCTGGAAGG TGGCCTATAC GGATGCCAGC 
TGGGAGACCA AAATGGTCTG GCACCGAACC AATACGATCC TGGGCTTCAG CGAAATGGAC 
ATCTACTGGG ACATCAGTCC GCAGACTCTT CCCGGGGAAT ATCGCATTCG CCATTCCGGG 
GAGTACAAGT ACATCCTGGG CGGAAAGTAT CCCTACGAGG GACTCACGCA TTCCTTCACC 
GTTAAGGAGG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_733367.1
CDS	83..2197
Translation	MANSKMAFLAFLAVSFLCGLVSATYKVGVGRADITGPPVEINFMGYANIKQVGRGIHTRVFARAFVVEDEKGNRVAFVSADAGMMGYGLKREVIKRLQARYGNIYHNDNVAISGTHTHGAPGGFLMHLLYDISILGFVPQTFEVMAQGLYLCIKRATDNLVDGRILLSKTTVLNVNINRSPSSYLRNPAEERAQYEHDTDKTLTQLRFVDLENNLLGAFNWYAVHATSMNNTNRLVTSDNVGYAALLLEKEYNPNKMPGKGKFVGAFCSSNLGDVSPNIMGPKCSISGNECDLLTSRCPTGEGDCFASGPGKDMFESTQILGQRLADAALGLLNEQSQESTAREVTGDVRFIHQFVDMPNYNGSTYNPLSRKVDKIRGCQPAMGYSFAAGTTDGPGAFSFEQGTTTDNPMWNFVRDFIAAPTQEDIKCHEPKPILLATGRATFPYEWQPKIVSDQLLKIGDVIIAAVPCEFTTMAGRRLRNQIRAAASAVGGIDTEVIIAGLTNIYTSYTVTPEEYQAQRYEAASTIFGPHTHSIYMDVFERLTKAMMRNETVDAGPSPPYMNDVMLSLNTGVLFDGHPINTDFGYVKSQPNKEYGINETVKVTYISGNPRNNLFTEKTYFTIERKINEDRWKVAYTDASWETKMVWHRTNTILGFSEMDIYWDISPQTLPGEYRIRHSGEYKYILGGKYPYEGLTHSFTVKED

Target ORF information:

RefSeq Version	NM_170488.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ceramidase, transcript variant B (CDase), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170488.2

ORF Insert Sequence:

ATGGCTAATA GCAAGATGGC CTTCTTGGCC TTTCTGGCCG TAAGTTTCCT GTGTGGATTG 
GTTAGTGCCA CCTACAAAGT GGGCGTGGGC CGCGCGGATA TCACAGGACC TCCAGTGGAG 
ATCAATTTCA TGGGCTATGC CAACATCAAG CAAGTGGGTC GTGGCATCCA CACCCGTGTC 
TTTGCCCGTG CCTTTGTGGT GGAGGATGAA AAGGGCAACC GAGTGGCCTT CGTGAGCGCA 
GATGCCGGAA TGATGGGCTA CGGATTGAAG AGGGAGGTGA TAAAACGACT CCAGGCACGT 
TATGGCAACA TCTACCACAA TGATAATGTG GCCATCAGTG GCACCCACAC GCACGGTGCT 
CCTGGCGGAT TCCTGATGCA TCTGCTCTAT GACATCTCCA TTCTGGGCTT TGTGCCTCAG 
ACGTTTGAGG TGATGGCTCA GGGACTGTAT CTGTGCATCA AAAGGGCGAC GGACAACCTG 
GTGGATGGTC GCATCTTGCT GTCCAAAACT ACTGTGCTAA ATGTTAACAT CAATCGTTCG 
CCCTCATCCT ACTTGAGGAA TCCCGCCGAG GAGCGTGCCC AATACGAGCA CGATACGGAT 
AAGACACTGA CCCAGCTGCG ATTTGTCGAC CTGGAAAACA ACCTCCTGGG CGCTTTCAAC 
TGGTATGCGG TGCATGCCAC CTCCATGAAC AATACCAACA GACTGGTGAC CAGCGACAAT 
GTGGGTTATG CCGCCCTGCT CCTGGAAAAG GAGTACAATC CGAACAAGAT GCCCGGAAAG 
GGCAAGTTCG TGGGTGCCTT CTGCTCATCC AACCTTGGCG ATGTGTCCCC CAATATAATG 
GGTCCCAAGT GCTCGATCTC CGGCAATGAG TGTGATCTAC TGACCTCCCG TTGTCCCACT 
GGCGAGGGAG ATTGCTTTGC CTCCGGACCC GGTAAGGATA TGTTCGAAAG CACCCAGATC 
TTGGGTCAAC GTCTGGCGGA TGCTGCTCTG GGACTGCTCA ACGAACAGAG CCAGGAGTCC 
ACGGCACGGG AGGTCACTGG AGATGTGAGG TTCATCCACC AGTTTGTGGA CATGCCCAAC 
TACAATGGCA GCACCTACAA TCCGCTGAGC AGGAAGGTCG ACAAGATCAG GGGATGTCAG 
CCGGCCATGG GCTATAGCTT TGCTGCTGGT ACCACCGATG GACCTGGAGC CTTCAGCTTC 
GAGCAGGGAA CCACCACGGA CAACCCCATG TGGAACTTTG TGCGCGACTT CATCGCTGCT 
CCCACGCAGG AGGACATCAA GTGCCATGAG CCCAAGCCCA TTCTTCTGGC CACCGGCAGG 
GCCACCTTTC CCTATGAGTG GCAGCCAAAG ATTGTCTCGG ATCAGCTACT AAAGATCGGC 
GATGTGATCA TAGCCGCCGT GCCCTGCGAG TTCACCACAA TGGCAGGACG TCGTCTGCGA 
AACCAGATTC GAGCAGCTGC CTCCGCCGTC GGAGGCATCG ACACCGAGGT AATCATCGCC 
GGACTGACCA ACATATACAC CAGCTACACG GTGACCCCGG AGGAGTACCA GGCGCAGCGT 
TACGAGGCCG CCTCCACGAT CTTCGGTCCC CATACCCACT CCATTTACAT GGACGTCTTC 
GAACGCCTGA CCAAGGCCAT GATGCGTAAT GAGACCGTGG ATGCGGGTCC AAGTCCGCCG 
TACATGAATG ATGTGATGCT ATCGCTGAAC ACCGGGGTTC TCTTCGACGG ACATCCCATA 
AACACCGACT TTGGCTACGT GAAGTCGCAG CCCAACAAGG AGTACGGCAT CAACGAGACG 
GTGAAGGTCA CCTACATATC CGGAAATCCG CGTAACAACC TCTTCACCGA GAAAACCTAC 
TTCACCATTG AGCGAAAGAT CAACGAGGAT CGCTGGAAGG TGGCCTATAC GGATGCCAGC 
TGGGAGACCA AAATGGTCTG GCACCGAACC AATACGATCC TGGGCTTCAG CGAAATGGAC 
ATCTACTGGG ACATCAGTCC GCAGACTCTT CCCGGGGAAT ATCGCATTCG CCATTCCGGG 
GAGTACAAGT ACATCCTGGG CGGAAAGTAT CCCTACGAGG GACTCACGCA TTCCTTCACC 
GTTAAGGAGG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CDase cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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