hspa5 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	hspa5
Entrez Gene ID	378848
Full Name	heat shock protein 5
Synonyms	cb865,fb60h09,fi36d04,wu:fb60h09,wu:fi36d04,zgc:55994,zgc:77606
General protein information	Preferred Names heat shock protein 5 Names 78 kDa glucose-regulated protein bip glucose-regulated protein, 78kDa heat shock 70kDa protein 5 (glucose-regulated protein)
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_213058.1	NP_998223.1	78 kDa glucose-regulated protein precursor

Pathways from BioSystems

Homologies

Caenorhabditis elegans (roundworm)	hsp-4	NP_495536.1
Schizosaccharomyces pombe (fission yeast)	bip1	NP_593245.1
Arabidopsis thaliana (thale cress)	BIP1	NP_198206.1
Homo sapiens (human)	HSPA5	NP_005338.1
Rattus norvegicus (Norway rat)	Hspa5	NP_037215.1
Danio rerio (zebrafish)	hspa5	NP_998223.1
Neurospora crassa	NCU03982	XP_956567.1
Xenopus tropicalis (tropical clawed frog)	LOC100492570	XP_002941690.1
	HSPA5	XP_001099110.1
Canis lupus familiaris (dog)	HSPA5	XP_863385.2
Bos taurus (cattle)	HSPA5	NP_001068616.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004192	XP_313085.3
Eremothecium gossypii	AGOS_ACR038W	NP_983441.1
Caenorhabditis elegans (roundworm)	hsp-3	NP_509019.1
Saccharomyces cerevisiae (baker's yeast)	KAR2	NP_012500.3
Kluyveromyces lactis	KLLA0D09559g	XP_453488.1
Arabidopsis thaliana (thale cress)	BIP2	NP_851119.1
Pan troglodytes (chimpanzee)	HSPA5	XP_520257.2
Mus musculus (house mouse)	Hspa5	NP_001156906.1
Gallus gallus (chicken)	HSPA5	NP_990822.1
Drosophila melanogaster (fruit fly)	Hsc70-3	NP_727564.1
Magnaporthe oryzae (rice blast fungus)	MGG_02503	XP_003709308.1
Oryza sativa (rice)	Os02g0115900	NP_001045675.1

Gene Ontology

The following hspa5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the hspa5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa00958	NM_213058.1 Latest version!	Danio rerio heat shock protein 5 (hspa5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

hspa5 ( NM_213058.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_998223.1 Danio rerio heat shock protein 5 (hspa5), mRNA.

Price & Availability↑

CloneID	ODa00958
Clone ID Related Accession (Same CDS sequence)	NM_213058.1
Accession Version	NM_213058.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1953bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-20
Organism	Danio rerio(zebrafish)
Product	78 kDa glucose-regulated protein precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC052971.1. On Jul 13, 2004 this sequence version replaced NM_213254.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC052971.1, AY648749.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA4476767 [ECO:0000348] ##Evidence-Data-END##

ATGCGGTTGC TTTGCCTGTT TTTGCTGGTG GCCGGCAGCG TGTTTGCCGA AGAGGACGAT 
AAGAAGGAGA GTGTTGGGAC AGTGATTGGG ATCGACCTCG GGACCACATA CTCCTGTGTT 
GGAGTCTACA AGAATGGCCG TGTTGAGATT ATTGCCAATG ACCAGGGAAA CCGCATCACT 
CCGTCATACG TGGCCTTTAC CACTGAAGGA GAGCGGCTCA TCGGAGATGC TGCGAAGAAC 
CAGCTCACAT CCAACCCTGA AAACACTGTG TTTGACGCCA AGAGGCTGAT CGGACGCACA 
TGGGGCGACT CTTCTGTGCA GCAGGACATC AAATACTTCC CCTTTAAGGT GATCGAGAAG 
AAAAACAAGC CTCACATCCA GCTGGACATC GGCTCTGGTC AGATGAAGAC GTTTGCACCG 
GAGGAAATTT CCGCCATGGT TTTGACCAAG ATGAAGGAAA CCGCAGAGGC TTATCTGGGA 
AAGAAGGTCA CTCATGCTGT GGTCACCGTT CCTGCTTATT TCAACGATGC TCAGCGTCAG 
GCCACTAAAG ATGCTGGAAC CATTGCTGGG CTGAATGTCA TGAGGATCAT CAATGAGCCT 
ACGGCGGCTG CCATTGCATA CGGTCTGGAC AAGAGGGACG GAGAGAAAAA CATCCTGGTG 
TTCGATCTGG GTGGTGGCAC CTTTGACGTG TCTCTGCTGA CCATCGATAA CGGCGTGTTT 
GAAGTGGTGG CCACAAACGG AGACACTCAC CTGGGCGGAG AAGACTTCGA CCAGCGCGTC 
ATGGAGCACT TCATCAAGCT GTACAAGAAG AAGACGGGCA AAGACGTGCG CAAAGACAAC 
CGCGCCGTGC AGAAGCTGCG CAGAGAGGTG GAGAAGGCTA AGAGAGCGCT GTCTGCCCAG 
CATCAGGCCC GCATCGAGAT CGAGTCCTTC TTTGAAGGAG AAGATTTCTC TGAGACTCTC 
ACCAGAGCCA AGTTTGAGGA GCTCAACATG GACTTGTTCC GCTCCACTAT GAAGCCGGTT 
CAGAAGGTTC TGGAGGACTC TGACCCGAAG AAGCCAGATA TCGATGAGAT CGTGCTGGTC 
GGCGGCTCCA CTCGTATCCC GAAGATCCAG CAGCTGGTGA AGGAGTTCTT CAACGGAAAA 
GAGCCGTCCA GAGGAATCAA CCCTGACGAG GCCGTGGCGT ACGGAGCTGC TGTCCAGGCT 
GGAGTCCTGT CCGGAGAGGA GGAGACCGGT GATCTGGTTC TTCTGGACGT GTGTCCGCTG 
ACTCTGGGCA TTGAGACTGT TGGAGGAGTG ATGACCAAAC TCATTCCCAG AAACACTGTT 
GTTCCCACCA AGAAATCCCA GATCTTCTCC ACTGCTTCCG ACAACCAGCC CACCGTCACT 
ATCAAAGTTT ATGAGGGCGA GCGTCCCCTG ACCAAAGACA ACCATCTGCT GGGCACCTTT 
GACCTGACAG GCATCCCTCC AGCACCTCGT GGTGTCCCAC AGATCGAGGT AACTTTCGAG 
ATCGACGTCA ACGGCATCCT GCGCGTCACC GCCGAAGACA AAGGCACCGG AAACAAAAAC 
AAGATCACCA TTACCAACGA CCAGAACCGG CTGACCCCTG AGGACATCGA GAGAATGGTG 
AACGAAGCCG AGAGATTCGC TGATGAGGAC AAGAAACTGA AGGAGAGAAT CGACAGCCGC 
AATGAATTGG AGAGCTACGC CTATTCCCTG AAGAACCAGA TCGGGGATAA AGAGAAATTA 
GGCGGAAAGT TATCCTCTGA AGACAAGGAG GCCATCGAGA AGGCAGTGGA GGAGAAGATC 
GAGTGGCTGG AGGCGCATCA GGACGCCGAT CTGGAGGAAT TCCAGGCCAA AAAGAAGGAG 
CTGGAGGAGG TGGTGCAGCC CATCGTCAGC AAACTGTACG GCAGTGCGGG AGGACCACCG 
CCTGAAGAGG CCGAAGAGAA GGACGAGCTG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_998223.1
CDS	139..2091
Translation	MRLLCLFLLVAGSVFAEEDDKKESVGTVIGIDLGTTYSCVGVYKNGRVEIIANDQGNRITPSYVAFTTEGERLIGDAAKNQLTSNPENTVFDAKRLIGRTWGDSSVQQDIKYFPFKVIEKKNKPHIQLDIGSGQMKTFAPEEISAMVLTKMKETAEAYLGKKVTHAVVTVPAYFNDAQRQATKDAGTIAGLNVMRIINEPTAAAIAYGLDKRDGEKNILVFDLGGGTFDVSLLTIDNGVFEVVATNGDTHLGGEDFDQRVMEHFIKLYKKKTGKDVRKDNRAVQKLRREVEKAKRALSAQHQARIEIESFFEGEDFSETLTRAKFEELNMDLFRSTMKPVQKVLEDSDPKKPDIDEIVLVGGSTRIPKIQQLVKEFFNGKEPSRGINPDEAVAYGAAVQAGVLSGEEETGDLVLLDVCPLTLGIETVGGVMTKLIPRNTVVPTKKSQIFSTASDNQPTVTIKVYEGERPLTKDNHLLGTFDLTGIPPAPRGVPQIEVTFEIDVNGILRVTAEDKGTGNKNKITITNDQNRLTPEDIERMVNEAERFADEDKKLKERIDSRNELESYAYSLKNQIGDKEKLGGKLSSEDKEAIEKAVEEKIEWLEAHQDADLEEFQAKKKELEEVVQPIVSKLYGSAGGPPPEEAEEKDEL

Target ORF information:

RefSeq Version	NM_213058.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio heat shock protein 5 (hspa5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_213058.1

ORF Insert Sequence:

ATGCGGTTGC TTTGCCTGTT TTTGCTGGTG GCCGGCAGCG TGTTTGCCGA AGAGGACGAT 
AAGAAGGAGA GTGTTGGGAC AGTGATTGGG ATCGACCTCG GGACCACATA CTCCTGTGTT 
GGAGTCTACA AGAATGGCCG TGTTGAGATT ATTGCCAATG ACCAGGGAAA CCGCATCACT 
CCGTCATACG TGGCCTTTAC CACTGAAGGA GAGCGGCTCA TCGGAGATGC TGCGAAGAAC 
CAGCTCACAT CCAACCCTGA AAACACTGTG TTTGACGCCA AGAGGCTGAT CGGACGCACA 
TGGGGCGACT CTTCTGTGCA GCAGGACATC AAATACTTCC CCTTTAAGGT GATCGAGAAG 
AAAAACAAGC CTCACATCCA GCTGGACATC GGCTCTGGTC AGATGAAGAC GTTTGCACCG 
GAGGAAATTT CCGCCATGGT TTTGACCAAG ATGAAGGAAA CCGCAGAGGC TTATCTGGGA 
AAGAAGGTCA CTCATGCTGT GGTCACCGTT CCTGCTTATT TCAACGATGC TCAGCGTCAG 
GCCACTAAAG ATGCTGGAAC CATTGCTGGG CTGAATGTCA TGAGGATCAT CAATGAGCCT 
ACGGCGGCTG CCATTGCATA CGGTCTGGAC AAGAGGGACG GAGAGAAAAA CATCCTGGTG 
TTCGATCTGG GTGGTGGCAC CTTTGACGTG TCTCTGCTGA CCATCGATAA CGGCGTGTTT 
GAAGTGGTGG CCACAAACGG AGACACTCAC CTGGGCGGAG AAGACTTCGA CCAGCGCGTC 
ATGGAGCACT TCATCAAGCT GTACAAGAAG AAGACGGGCA AAGACGTGCG CAAAGACAAC 
CGCGCCGTGC AGAAGCTGCG CAGAGAGGTG GAGAAGGCTA AGAGAGCGCT GTCTGCCCAG 
CATCAGGCCC GCATCGAGAT CGAGTCCTTC TTTGAAGGAG AAGATTTCTC TGAGACTCTC 
ACCAGAGCCA AGTTTGAGGA GCTCAACATG GACTTGTTCC GCTCCACTAT GAAGCCGGTT 
CAGAAGGTTC TGGAGGACTC TGACCCGAAG AAGCCAGATA TCGATGAGAT CGTGCTGGTC 
GGCGGCTCCA CTCGTATCCC GAAGATCCAG CAGCTGGTGA AGGAGTTCTT CAACGGAAAA 
GAGCCGTCCA GAGGAATCAA CCCTGACGAG GCCGTGGCGT ACGGAGCTGC TGTCCAGGCT 
GGAGTCCTGT CCGGAGAGGA GGAGACCGGT GATCTGGTTC TTCTGGACGT GTGTCCGCTG 
ACTCTGGGCA TTGAGACTGT TGGAGGAGTG ATGACCAAAC TCATTCCCAG AAACACTGTT 
GTTCCCACCA AGAAATCCCA GATCTTCTCC ACTGCTTCCG ACAACCAGCC CACCGTCACT 
ATCAAAGTTT ATGAGGGCGA GCGTCCCCTG ACCAAAGACA ACCATCTGCT GGGCACCTTT 
GACCTGACAG GCATCCCTCC AGCACCTCGT GGTGTCCCAC AGATCGAGGT AACTTTCGAG 
ATCGACGTCA ACGGCATCCT GCGCGTCACC GCCGAAGACA AAGGCACCGG AAACAAAAAC 
AAGATCACCA TTACCAACGA CCAGAACCGG CTGACCCCTG AGGACATCGA GAGAATGGTG 
AACGAAGCCG AGAGATTCGC TGATGAGGAC AAGAAACTGA AGGAGAGAAT CGACAGCCGC 
AATGAATTGG AGAGCTACGC CTATTCCCTG AAGAACCAGA TCGGGGATAA AGAGAAATTA 
GGCGGAAAGT TATCCTCTGA AGACAAGGAG GCCATCGAGA AGGCAGTGGA GGAGAAGATC 
GAGTGGCTGG AGGCGCATCA GGACGCCGAT CTGGAGGAAT TCCAGGCCAA AAAGAAGGAG 
CTGGAGGAGG TGGTGCAGCC CATCGTCAGC AAACTGTACG GCAGTGCGGG AGGACCACCG 
CCTGAAGAGG CCGAAGAGAA GGACGAGCTG TAG