Hspa5 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hspa5
Entrez Gene ID	14828
Full Name	heat shock protein 5
Synonyms	AL022860,AU019543,Bip,D2Wsu141e,D2Wsu17e,Grp78,Hsce70,SEZ-7,Sez7,baffled,mBiP
General protein information	Preferred Names heat shock protein 5 Names 78 kDa glucose-regulated protein GRP-78 XAP-1 antigen glucose regulated protein, 78 kDa heat shock 70 kDa protein 5 heat shock 70kD protein 5 (glucose-regulated protein, 78kD) immunoglobulin heavy chain-binding protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2 B\|2 22.94 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_022310.3	NP_071705.3	endoplasmic reticulum chaperone BiP precursor
NM_001163434.1	NP_001156906.1	endoplasmic reticulum chaperone BiP precursor

Pathways from BioSystems

Homologies

Caenorhabditis elegans (roundworm)	hsp-4	NP_495536.1
Schizosaccharomyces pombe (fission yeast)	bip1	NP_593245.1
Arabidopsis thaliana (thale cress)	BIP1	NP_198206.1
Homo sapiens (human)	HSPA5	NP_005338.1
Rattus norvegicus (Norway rat)	Hspa5	NP_037215.1
Danio rerio (zebrafish)	hspa5	NP_998223.1
Neurospora crassa	NCU03982	XP_956567.1
Xenopus tropicalis (tropical clawed frog)	LOC100492570	XP_002941690.1
	HSPA5	XP_001099110.1
Canis lupus familiaris (dog)	HSPA5	XP_863385.2
Bos taurus (cattle)	HSPA5	NP_001068616.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004192	XP_313085.3
Eremothecium gossypii	AGOS_ACR038W	NP_983441.1
Caenorhabditis elegans (roundworm)	hsp-3	NP_509019.1
Saccharomyces cerevisiae (baker's yeast)	KAR2	NP_012500.3
Kluyveromyces lactis	KLLA0D09559g	XP_453488.1
Arabidopsis thaliana (thale cress)	BIP2	NP_851119.1
Pan troglodytes (chimpanzee)	HSPA5	XP_520257.2
Mus musculus (house mouse)	Hspa5	NP_001156906.1
Gallus gallus (chicken)	HSPA5	NP_990822.1
Drosophila melanogaster (fruit fly)	Hsc70-3	NP_727564.1
Magnaporthe oryzae (rice blast fungus)	MGG_02503	XP_003709308.1
Oryza sativa (rice)	Os02g0115900	NP_001045675.1

Gene Ontology

The following Hspa5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hspa5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu16064	NM_001163434.1 Latest version!	Mus musculus heat shock protein 5 (Hspa5), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$321.30 $459.00
OMu16064	NM_022310.3 Latest version!	Mus musculus heat shock protein 5 (Hspa5), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$321.30 $459.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hspa5 ( NM_022310.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_071705.3 Mus musculus heat shock protein 5 (Hspa5), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu16064
Clone ID Related Accession (Same CDS sequence)	NM_022310.3 , NM_001163434.1
Accession Version	NM_022310.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1968bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Mus musculus(house mouse)
Product	endoplasmic reticulum chaperone BiP precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL929106.5 and AK152521.1. On Jul 21, 2009 this sequence version replaced NM_022310.2. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660815.54083.1, SRR1660817.183933.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGATGAAGT TCACTGTGGT GGCGGCGGCG TTGCTGCTGC TGGGCGCGGT GCGGGCCGAG 
GAGGAGGACA AGAAGGAGGA TGTGGGCACG GTGGTCGGCA TCGACTTGGG GACCACCTAT 
TCCTGCGTCG GTGTGTTCAA GAACGGCCGC GTGGAGATCA TAGCCAACGA TCAGGGCAAC 
CGCATCACGC CGTCGTATGT GGCCTTCACT CCTGAAGGGG AGCGTCTGAT TGGCGATGCG 
GCCAAGAACC AACTCACGTC CAACCCCGAG AACACGGTCT TCGATGCCAA GCGCCTCATC 
GGACGCACTT GGAATGACCC TTCGGTGCAG CAGGACATCA AGTTCTTGCC ATTCAAGGTG 
GTTGAAAAGA AAACTAAACC GTACATTCAA GTTGATATTG GAGGTGGGCA AACCAAGACA 
TTTGCCCCAG AAGAAATTTC TGCCATGGTT CTCACTAAAA TGAAGGAGAC TGCTGAGGCG 
TATTTGGGAA AGAAGGTTAC CCATGCAGTT GTTACTGTAC CAGCTTACTT CAATGATGCC 
CAGCGACAAG CAACCAAAGA TGCTGGCACT ATTGCTGGAC TGAATGTCAT GAGGATCATC 
AATGAGCCTA CAGCAGCTGC TATTGCATAT GGCCTGGATA AGAGAGAGGG AGAGAAGAAC 
ATCCTTGTGT TTGACCTGGG TGGCGGCACC TTCGATGTGT CTCTTCTCAC CATTGACAAT 
GGTGTCTTTG AAGTGGTGGC CACTAATGGA GATACTCACC TGGGTGGGGA AGACTTTGAT 
CAGCGGGTCA TGGAACACTT CATCAAGTTG TACAAAAAGA AAACTGGTAA AGATGTTAGG 
AAAGACAACA GAGCTGTGCA GAAACTCCGG CGTGAGGTAG AAAAGGCTAA GAGAGCCTTG 
TCTTCTCAGC ATCAAGCAAG GATTGAAATT GAGTCCTTCT TCGAAGGAGA AGACTTCTCA 
GAGACCCTTA CTCGGGCCAA ATTTGAAGAG CTGAACATGG ACCTGTTCCG CTCTACCATG 
AAGCCTGTCC AGAAAGTGTT GGAAGACTCT GATCTGAAGA AATCTGATAT TGATGAAATT 
GTTCTGGTTG GTGGATCTAC TCGAATTCCA AAGATTCAGC AACTGGTGAA AGAGTTCTTC 
AATGGCAAGG AGCCATCCCG TGGCATAAAC CCCGATGAGG CTGTAGCCTA TGGTGCCGCT 
GTCCAGGCTG GTGTCCTCTC TGGTGATCAG GATACAGGTG ATCTGGTACT GCTTGATGTT 
TGTCCCCTTA CACTTGGTAT TGAAACTGTG GGAGGAGTCA TGACAAAACT GATTCCAAGG 
AACACTGTGG TACCCACCAA GAAGTCTCAG ATCTTCTCCA CGGCTTCCGA TAATCAGCCA 
ACTGTAACAA TCAAGGTCTA TGAAGGTGAA CGACCCCTAA CAAAAGACAA TCATCTTCTG 
GGTACATTTG ATCTGACTGG AATTCCTCCT GCTCCCCGTG GAGTTCCCCA GATTGAAGTC 
ACTTTTGAGA TAGATGTTAA TGGTATTCTC CGAGTGACAG CTGAAGACAA AGGTACAGGA 
AACAAAAACA AAATCACAAT TACCAATGAC CAAAACCGCC TGACACCTGA AGAAATTGAA 
AGGATGGTTA ATGATGCTGA GAAGTTTGCT GAGGAAGACA AAAAGCTCAA AGAGCGCATT 
GACACCAGGA ATGAATTGGA AAGCTATGCT TATTCTCTCA AGAACCAGAT TGGAGATAAA 
GAAAAGCTGG GAGGTAAACT TTCTTCTGAA GATAAAGAAA CCATGGAAAA AGCTGTAGAG 
GAAAAGATTG AATGGCTGGA AAGCCACCAG GATGCGGACA TTGAAGACTT TAAAGCCAAA 
AAGAAGGAAC TAGAAGAAAT TGTTCAGCCA ATTATCAGCA AACTCTATGG AAGTGGAGGC 
CCTCCCCCAA CTGGTGAAGA GGATACATCA GAAAAAGATG AGTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_071705.3
CDS	233..2200
Translation	MMKFTVVAAALLLLGAVRAEEEDKKEDVGTVVGIDLGTTYSCVGVFKNGRVEIIANDQGNRITPSYVAFTPEGERLIGDAAKNQLTSNPENTVFDAKRLIGRTWNDPSVQQDIKFLPFKVVEKKTKPYIQVDIGGGQTKTFAPEEISAMVLTKMKETAEAYLGKKVTHAVVTVPAYFNDAQRQATKDAGTIAGLNVMRIINEPTAAAIAYGLDKREGEKNILVFDLGGGTFDVSLLTIDNGVFEVVATNGDTHLGGEDFDQRVMEHFIKLYKKKTGKDVRKDNRAVQKLRREVEKAKRALSSQHQARIEIESFFEGEDFSETLTRAKFEELNMDLFRSTMKPVQKVLEDSDLKKSDIDEIVLVGGSTRIPKIQQLVKEFFNGKEPSRGINPDEAVAYGAAVQAGVLSGDQDTGDLVLLDVCPLTLGIETVGGVMTKLIPRNTVVPTKKSQIFSTASDNQPTVTIKVYEGERPLTKDNHLLGTFDLTGIPPAPRGVPQIEVTFEIDVNGILRVTAEDKGTGNKNKITITNDQNRLTPEEIERMVNDAEKFAEEDKKLKERIDTRNELESYAYSLKNQIGDKEKLGGKLSSEDKETMEKAVEEKIEWLESHQDADIEDFKAKKKELEEIVQPIISKLYGSGGPPPTGEEDTSEKDEL

Target ORF information:

RefSeq Version	NM_022310.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus heat shock protein 5 (Hspa5), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022310.3

ORF Insert Sequence:

ATGATGAAGT TCACTGTGGT GGCGGCGGCG TTGCTGCTGC TGGGCGCGGT GCGGGCCGAG 
GAGGAGGACA AGAAGGAGGA TGTGGGCACG GTGGTCGGCA TCGACTTGGG GACCACCTAT 
TCCTGCGTCG GTGTGTTCAA GAACGGCCGC GTGGAGATCA TAGCCAACGA TCAGGGCAAC 
CGCATCACGC CGTCGTATGT GGCCTTCACT CCTGAAGGGG AGCGTCTGAT TGGCGATGCG 
GCCAAGAACC AACTCACGTC CAACCCCGAG AACACGGTCT TCGATGCCAA GCGCCTCATC 
GGACGCACTT GGAATGACCC TTCGGTGCAG CAGGACATCA AGTTCTTGCC ATTCAAGGTG 
GTTGAAAAGA AAACTAAACC GTACATTCAA GTTGATATTG GAGGTGGGCA AACCAAGACA 
TTTGCCCCAG AAGAAATTTC TGCCATGGTT CTCACTAAAA TGAAGGAGAC TGCTGAGGCG 
TATTTGGGAA AGAAGGTTAC CCATGCAGTT GTTACTGTAC CAGCTTACTT CAATGATGCC 
CAGCGACAAG CAACCAAAGA TGCTGGCACT ATTGCTGGAC TGAATGTCAT GAGGATCATC 
AATGAGCCTA CAGCAGCTGC TATTGCATAT GGCCTGGATA AGAGAGAGGG AGAGAAGAAC 
ATCCTTGTGT TTGACCTGGG TGGCGGCACC TTCGATGTGT CTCTTCTCAC CATTGACAAT 
GGTGTCTTTG AAGTGGTGGC CACTAATGGA GATACTCACC TGGGTGGGGA AGACTTTGAT 
CAGCGGGTCA TGGAACACTT CATCAAGTTG TACAAAAAGA AAACTGGTAA AGATGTTAGG 
AAAGACAACA GAGCTGTGCA GAAACTCCGG CGTGAGGTAG AAAAGGCTAA GAGAGCCTTG 
TCTTCTCAGC ATCAAGCAAG GATTGAAATT GAGTCCTTCT TCGAAGGAGA AGACTTCTCA 
GAGACCCTTA CTCGGGCCAA ATTTGAAGAG CTGAACATGG ACCTGTTCCG CTCTACCATG 
AAGCCTGTCC AGAAAGTGTT GGAAGACTCT GATCTGAAGA AATCTGATAT TGATGAAATT 
GTTCTGGTTG GTGGATCTAC TCGAATTCCA AAGATTCAGC AACTGGTGAA AGAGTTCTTC 
AATGGCAAGG AGCCATCCCG TGGCATAAAC CCCGATGAGG CTGTAGCCTA TGGTGCCGCT 
GTCCAGGCTG GTGTCCTCTC TGGTGATCAG GATACAGGTG ATCTGGTACT GCTTGATGTT 
TGTCCCCTTA CACTTGGTAT TGAAACTGTG GGAGGAGTCA TGACAAAACT GATTCCAAGG 
AACACTGTGG TACCCACCAA GAAGTCTCAG ATCTTCTCCA CGGCTTCCGA TAATCAGCCA 
ACTGTAACAA TCAAGGTCTA TGAAGGTGAA CGACCCCTAA CAAAAGACAA TCATCTTCTG 
GGTACATTTG ATCTGACTGG AATTCCTCCT GCTCCCCGTG GAGTTCCCCA GATTGAAGTC 
ACTTTTGAGA TAGATGTTAA TGGTATTCTC CGAGTGACAG CTGAAGACAA AGGTACAGGA 
AACAAAAACA AAATCACAAT TACCAATGAC CAAAACCGCC TGACACCTGA AGAAATTGAA 
AGGATGGTTA ATGATGCTGA GAAGTTTGCT GAGGAAGACA AAAAGCTCAA AGAGCGCATT 
GACACCAGGA ATGAATTGGA AAGCTATGCT TATTCTCTCA AGAACCAGAT TGGAGATAAA 
GAAAAGCTGG GAGGTAAACT TTCTTCTGAA GATAAAGAAA CCATGGAAAA AGCTGTAGAG 
GAAAAGATTG AATGGCTGGA AAGCCACCAG GATGCGGACA TTGAAGACTT TAAAGCCAAA 
AAGAAGGAAC TAGAAGAAAT TGTTCAGCCA ATTATCAGCA AACTCTATGG AAGTGGAGGC 
CCTCCCCCAA CTGGTGAAGA GGATACATCA GAAAAAGATG AGTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hspa5 ( NM_001163434.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001156906.1 Mus musculus heat shock protein 5 (Hspa5), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu16064
Clone ID Related Accession (Same CDS sequence)	NM_022310.3 , NM_001163434.1
Accession Version	NM_001163434.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1968bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Mus musculus(house mouse)
Product	endoplasmic reticulum chaperone BiP precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL929106.5 and AK148539.1. Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK160821.1, AK077463.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGATGAAGT TCACTGTGGT GGCGGCGGCG TTGCTGCTGC TGGGCGCGGT GCGGGCCGAG 
GAGGAGGACA AGAAGGAGGA TGTGGGCACG GTGGTCGGCA TCGACTTGGG GACCACCTAT 
TCCTGCGTCG GTGTGTTCAA GAACGGCCGC GTGGAGATCA TAGCCAACGA TCAGGGCAAC 
CGCATCACGC CGTCGTATGT GGCCTTCACT CCTGAAGGGG AGCGTCTGAT TGGCGATGCG 
GCCAAGAACC AACTCACGTC CAACCCCGAG AACACGGTCT TCGATGCCAA GCGCCTCATC 
GGACGCACTT GGAATGACCC TTCGGTGCAG CAGGACATCA AGTTCTTGCC ATTCAAGGTG 
GTTGAAAAGA AAACTAAACC GTACATTCAA GTTGATATTG GAGGTGGGCA AACCAAGACA 
TTTGCCCCAG AAGAAATTTC TGCCATGGTT CTCACTAAAA TGAAGGAGAC TGCTGAGGCG 
TATTTGGGAA AGAAGGTTAC CCATGCAGTT GTTACTGTAC CAGCTTACTT CAATGATGCC 
CAGCGACAAG CAACCAAAGA TGCTGGCACT ATTGCTGGAC TGAATGTCAT GAGGATCATC 
AATGAGCCTA CAGCAGCTGC TATTGCATAT GGCCTGGATA AGAGAGAGGG AGAGAAGAAC 
ATCCTTGTGT TTGACCTGGG TGGCGGCACC TTCGATGTGT CTCTTCTCAC CATTGACAAT 
GGTGTCTTTG AAGTGGTGGC CACTAATGGA GATACTCACC TGGGTGGGGA AGACTTTGAT 
CAGCGGGTCA TGGAACACTT CATCAAGTTG TACAAAAAGA AAACTGGTAA AGATGTTAGG 
AAAGACAACA GAGCTGTGCA GAAACTCCGG CGTGAGGTAG AAAAGGCTAA GAGAGCCTTG 
TCTTCTCAGC ATCAAGCAAG GATTGAAATT GAGTCCTTCT TCGAAGGAGA AGACTTCTCA 
GAGACCCTTA CTCGGGCCAA ATTTGAAGAG CTGAACATGG ACCTGTTCCG CTCTACCATG 
AAGCCTGTCC AGAAAGTGTT GGAAGACTCT GATCTGAAGA AATCTGATAT TGATGAAATT 
GTTCTGGTTG GTGGATCTAC TCGAATTCCA AAGATTCAGC AACTGGTGAA AGAGTTCTTC 
AATGGCAAGG AGCCATCCCG TGGCATAAAC CCCGATGAGG CTGTAGCCTA TGGTGCCGCT 
GTCCAGGCTG GTGTCCTCTC TGGTGATCAG GATACAGGTG ATCTGGTACT GCTTGATGTT 
TGTCCCCTTA CACTTGGTAT TGAAACTGTG GGAGGAGTCA TGACAAAACT GATTCCAAGG 
AACACTGTGG TACCCACCAA GAAGTCTCAG ATCTTCTCCA CGGCTTCCGA TAATCAGCCA 
ACTGTAACAA TCAAGGTCTA TGAAGGTGAA CGACCCCTAA CAAAAGACAA TCATCTTCTG 
GGTACATTTG ATCTGACTGG AATTCCTCCT GCTCCCCGTG GAGTTCCCCA GATTGAAGTC 
ACTTTTGAGA TAGATGTTAA TGGTATTCTC CGAGTGACAG CTGAAGACAA AGGTACAGGA 
AACAAAAACA AAATCACAAT TACCAATGAC CAAAACCGCC TGACACCTGA AGAAATTGAA 
AGGATGGTTA ATGATGCTGA GAAGTTTGCT GAGGAAGACA AAAAGCTCAA AGAGCGCATT 
GACACCAGGA ATGAATTGGA AAGCTATGCT TATTCTCTCA AGAACCAGAT TGGAGATAAA 
GAAAAGCTGG GAGGTAAACT TTCTTCTGAA GATAAAGAAA CCATGGAAAA AGCTGTAGAG 
GAAAAGATTG AATGGCTGGA AAGCCACCAG GATGCGGACA TTGAAGACTT TAAAGCCAAA 
AAGAAGGAAC TAGAAGAAAT TGTTCAGCCA ATTATCAGCA AACTCTATGG AAGTGGAGGC 
CCTCCCCCAA CTGGTGAAGA GGATACATCA GAAAAAGATG AGTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001156906.1
CDS	151..2118
Translation	MMKFTVVAAALLLLGAVRAEEEDKKEDVGTVVGIDLGTTYSCVGVFKNGRVEIIANDQGNRITPSYVAFTPEGERLIGDAAKNQLTSNPENTVFDAKRLIGRTWNDPSVQQDIKFLPFKVVEKKTKPYIQVDIGGGQTKTFAPEEISAMVLTKMKETAEAYLGKKVTHAVVTVPAYFNDAQRQATKDAGTIAGLNVMRIINEPTAAAIAYGLDKREGEKNILVFDLGGGTFDVSLLTIDNGVFEVVATNGDTHLGGEDFDQRVMEHFIKLYKKKTGKDVRKDNRAVQKLRREVEKAKRALSSQHQARIEIESFFEGEDFSETLTRAKFEELNMDLFRSTMKPVQKVLEDSDLKKSDIDEIVLVGGSTRIPKIQQLVKEFFNGKEPSRGINPDEAVAYGAAVQAGVLSGDQDTGDLVLLDVCPLTLGIETVGGVMTKLIPRNTVVPTKKSQIFSTASDNQPTVTIKVYEGERPLTKDNHLLGTFDLTGIPPAPRGVPQIEVTFEIDVNGILRVTAEDKGTGNKNKITITNDQNRLTPEEIERMVNDAEKFAEEDKKLKERIDTRNELESYAYSLKNQIGDKEKLGGKLSSEDKETMEKAVEEKIEWLESHQDADIEDFKAKKKELEEIVQPIISKLYGSGGPPPTGEEDTSEKDEL

Target ORF information:

RefSeq Version	NM_001163434.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus heat shock protein 5 (Hspa5), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001163434.1

ORF Insert Sequence:

ATGATGAAGT TCACTGTGGT GGCGGCGGCG TTGCTGCTGC TGGGCGCGGT GCGGGCCGAG 
GAGGAGGACA AGAAGGAGGA TGTGGGCACG GTGGTCGGCA TCGACTTGGG GACCACCTAT 
TCCTGCGTCG GTGTGTTCAA GAACGGCCGC GTGGAGATCA TAGCCAACGA TCAGGGCAAC 
CGCATCACGC CGTCGTATGT GGCCTTCACT CCTGAAGGGG AGCGTCTGAT TGGCGATGCG 
GCCAAGAACC AACTCACGTC CAACCCCGAG AACACGGTCT TCGATGCCAA GCGCCTCATC 
GGACGCACTT GGAATGACCC TTCGGTGCAG CAGGACATCA AGTTCTTGCC ATTCAAGGTG 
GTTGAAAAGA AAACTAAACC GTACATTCAA GTTGATATTG GAGGTGGGCA AACCAAGACA 
TTTGCCCCAG AAGAAATTTC TGCCATGGTT CTCACTAAAA TGAAGGAGAC TGCTGAGGCG 
TATTTGGGAA AGAAGGTTAC CCATGCAGTT GTTACTGTAC CAGCTTACTT CAATGATGCC 
CAGCGACAAG CAACCAAAGA TGCTGGCACT ATTGCTGGAC TGAATGTCAT GAGGATCATC 
AATGAGCCTA CAGCAGCTGC TATTGCATAT GGCCTGGATA AGAGAGAGGG AGAGAAGAAC 
ATCCTTGTGT TTGACCTGGG TGGCGGCACC TTCGATGTGT CTCTTCTCAC CATTGACAAT 
GGTGTCTTTG AAGTGGTGGC CACTAATGGA GATACTCACC TGGGTGGGGA AGACTTTGAT 
CAGCGGGTCA TGGAACACTT CATCAAGTTG TACAAAAAGA AAACTGGTAA AGATGTTAGG 
AAAGACAACA GAGCTGTGCA GAAACTCCGG CGTGAGGTAG AAAAGGCTAA GAGAGCCTTG 
TCTTCTCAGC ATCAAGCAAG GATTGAAATT GAGTCCTTCT TCGAAGGAGA AGACTTCTCA 
GAGACCCTTA CTCGGGCCAA ATTTGAAGAG CTGAACATGG ACCTGTTCCG CTCTACCATG 
AAGCCTGTCC AGAAAGTGTT GGAAGACTCT GATCTGAAGA AATCTGATAT TGATGAAATT 
GTTCTGGTTG GTGGATCTAC TCGAATTCCA AAGATTCAGC AACTGGTGAA AGAGTTCTTC 
AATGGCAAGG AGCCATCCCG TGGCATAAAC CCCGATGAGG CTGTAGCCTA TGGTGCCGCT 
GTCCAGGCTG GTGTCCTCTC TGGTGATCAG GATACAGGTG ATCTGGTACT GCTTGATGTT 
TGTCCCCTTA CACTTGGTAT TGAAACTGTG GGAGGAGTCA TGACAAAACT GATTCCAAGG 
AACACTGTGG TACCCACCAA GAAGTCTCAG ATCTTCTCCA CGGCTTCCGA TAATCAGCCA 
ACTGTAACAA TCAAGGTCTA TGAAGGTGAA CGACCCCTAA CAAAAGACAA TCATCTTCTG 
GGTACATTTG ATCTGACTGG AATTCCTCCT GCTCCCCGTG GAGTTCCCCA GATTGAAGTC 
ACTTTTGAGA TAGATGTTAA TGGTATTCTC CGAGTGACAG CTGAAGACAA AGGTACAGGA 
AACAAAAACA AAATCACAAT TACCAATGAC CAAAACCGCC TGACACCTGA AGAAATTGAA 
AGGATGGTTA ATGATGCTGA GAAGTTTGCT GAGGAAGACA AAAAGCTCAA AGAGCGCATT 
GACACCAGGA ATGAATTGGA AAGCTATGCT TATTCTCTCA AGAACCAGAT TGGAGATAAA 
GAAAAGCTGG GAGGTAAACT TTCTTCTGAA GATAAAGAAA CCATGGAAAA AGCTGTAGAG 
GAAAAGATTG AATGGCTGGA AAGCCACCAG GATGCGGACA TTGAAGACTT TAAAGCCAAA 
AAGAAGGAAC TAGAAGAAAT TGTTCAGCCA ATTATCAGCA AACTCTATGG AAGTGGAGGC 
CCTCCCCCAA CTGGTGAAGA GGATACATCA GAAAAAGATG AGTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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Hspa5 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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