HSPA5 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	HSPA5
Entrez Gene ID	415113
Full Name	heat shock protein family A (Hsp70) member 5
Synonyms	grp78
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024998380.1	XP_024854148.1	endoplasmic reticulum chaperone BiP isoform X1
NM_001075148.1	NP_001068616.1	endoplasmic reticulum chaperone BiP precursor

Pathways from BioSystems

Homologies

Caenorhabditis elegans (roundworm)	hsp-4	NP_495536.1
Schizosaccharomyces pombe (fission yeast)	bip1	NP_593245.1
Arabidopsis thaliana (thale cress)	BIP1	NP_198206.1
Homo sapiens (human)	HSPA5	NP_005338.1
Rattus norvegicus (Norway rat)	Hspa5	NP_037215.1
Danio rerio (zebrafish)	hspa5	NP_998223.1
Neurospora crassa	NCU03982	XP_956567.1
Xenopus tropicalis (tropical clawed frog)	LOC100492570	XP_002941690.1
	HSPA5	XP_001099110.1
Canis lupus familiaris (dog)	HSPA5	XP_863385.2
Bos taurus (cattle)	HSPA5	NP_001068616.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004192	XP_313085.3
Eremothecium gossypii	AGOS_ACR038W	NP_983441.1
Caenorhabditis elegans (roundworm)	hsp-3	NP_509019.1
Saccharomyces cerevisiae (baker's yeast)	KAR2	NP_012500.3
Kluyveromyces lactis	KLLA0D09559g	XP_453488.1
Arabidopsis thaliana (thale cress)	BIP2	NP_851119.1
Pan troglodytes (chimpanzee)	HSPA5	XP_520257.2
Mus musculus (house mouse)	Hspa5	NP_001156906.1
Gallus gallus (chicken)	HSPA5	NP_990822.1
Drosophila melanogaster (fruit fly)	Hsc70-3	NP_727564.1
Magnaporthe oryzae (rice blast fungus)	MGG_02503	XP_003709308.1
Oryza sativa (rice)	Os02g0115900	NP_001045675.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

AB5 subtilase cytotoxin inactivates the endoplasmic reticulum chaperone BiP.
Paton AW, Beddoe T, Thorpe CM, Whisstock JC, Wilce MC, Rossjohn J, Talbot UM, Paton JC
Nature443(7111)548-52(2006 Oct)

Characterization of 954 bovine full-CDS cDNA sequences.
Harhay GP, Sonstegard TS, Keele JW, Heaton MP, Clawson ML, Snelling WM, Wiedmann RT, Van Tassell CP, Smith TP
BMC genomics6166(2005 Nov)

Localization of the chaperone proteins GRP78 and HSP60 on the luminal surface of bovine oviduct epithelial cells and their association with spermatozoa.
Boilard M, Reyes-Moreno C, Lachance C, Massicotte L, Bailey JL, Sirard MA, Leclerc P
Biology of reproduction71(6)1879-89(2004 Dec)

Monitoring gene expression changes in bovine oviduct epithelial cells during the oestrous cycle.
Bauersachs S, Rehfeld S, Ulbrich SE, Mallok S, Prelle K, Wenigerkind H, Einspanier R, Blum H, Wolf E
Journal of molecular endocrinology32(2)449-66(2004 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

BiP is a master regulator of endoplasmic reticulum function, and its cleavage by subtilase cytotoxin represents a previously unknown trigger for cell death
Title: AB5 subtilase cytotoxin inactivates the endoplasmic reticulum chaperone BiP.

This paper reports the localization of both GRP78 and HSP60 on the luminal/apical surface of oviduct epithelial cells, their binding to spermatozoa, and the presence of endogenous HSP60 in the sperm midpiece.
Title: Localization of the chaperone proteins GRP78 and HSP60 on the luminal surface of bovine oviduct epithelial cells and their association with spermatozoa.

The following HSPA5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HSPA5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa42804	NM_001075148.1 Latest version!	Bos taurus heat shock protein family A (Hsp70) member 5 (HSPA5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa42804	XM_024998380.1 Latest version!	Bos taurus heat shock protein family A (Hsp70) member 5 (HSPA5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HSPA5 ( NM_001075148.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001068616.1 Bos taurus heat shock protein family A (Hsp70) member 5 (HSPA5), mRNA.

Price & Availability↑

CloneID	OBa42804
Clone ID Related Accession (Same CDS sequence)	XM_024998380.1 , NM_001075148.1
Accession Version	NM_001075148.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1968bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Bos taurus(cattle)
Product	endoplasmic reticulum chaperone BiP precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC119953.1. On Oct 4, 2006 this sequence version replaced XM_867578.1. ##Evidence-Data-START## Transcript exon combination :: BC119953.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145419, SAMN03145442 [ECO:0000348] ##Evidence-Data-END##

ATGAAGCTGT CCCTGGTGGC TGCGGTGCTG CTGCTGCTGC TTGGCACGGC ACGCGCGGAG 
GAGGAGGACA AGAAGGAGGA CGTGGGCACG GTGGTCGGCA TCGACCTGGG TACCACCTAC 
TCCTGCGTCG GGGTGTTCAA GAACGGCCGC GTGGAGATCA TCGCCAACGA TCAAGGCAAC 
CGCATCACGC CGTCTTATGT GGCTTTCACT CCTGAAGGGG AGCGTCTGAT CGGCGATGCA 
GCCAAGAACC AGCTCACCTC CAATCCGGAG AACACAGTCT TCGACGCCAA GCGACTCATC 
GGCCGGACGT GGAATGACCC GTCCGTGCAG CAGGACATCA AGTTCTTGCC TTTCAAGGTG 
GTTGAAAAGA AAACTAAACC ATACATTCAA GTTGATGTTG GAGGTGGGCA AACAAAGACA 
TTTGCTCCTG AAGAAATTTC TGCCATGGTT CTCACTAAAA TGAAGGAAAC TGCTGAGGCT 
TATTTGGGAA AGAAGGTTAC TCATGCAGTT GTTACTGTAC CAGCATATTT CAATGATGCC 
CAACGCCAGG CAACCAAAGA TGCTGGAACT ATTGCTGGAC TGAATGTCAT GAGGATCATC 
AATGAGCCTA CAGCAGCTGC TATTGCTTAT GGCCTGGATA AGAGGGAAGG GGAGAAGAAC 
ATCCTGGTGT TTGACCTGGG TGGTGGAACC TTTGATGTGT CTCTTCTCAC CATTGATAAT 
GGTGTCTTTG AAGTCGTGGC CACTAATGGA GATACTCATC TGGGTGGAGA AGACTTTGAC 
CAGCGTGTCA TGGAACACTT CATCAAACTC TACAAAAAGA AGACTGGCAA AGATGTTCGG 
AAGGACAACA GAGCTGTGCA GAAACTCCGG CGTGAGGTAG AAAAGGCCAA ACGGGCCCTG 
TCTTCCCAAC ATCAAGCAAG AATTGAAATT GAGTCCTTCT ATGAAGGAGA AGACTTCTCG 
GAGACCCTGA CTCGGGCTAA ATTTGAAGAG CTAAACATGG ACCTGTTCCG TTCCACCATG 
AAGCCTGTCC AGAAAGTGTT GGAAGATTCT GATTTAAAGA AGTCTGATAT TGATGAAATT 
GTTCTTGTTG GTGGCTCTAC TAGAATCCCA AAGATTCAAC AGCTGGTTAA AGAGTTTTTT 
AATGGCAAGG AGCCATCCCG TGGCATAAAC CCAGATGAGG CTGTGGCATA TGGTGCCGCT 
GTCCAGGCTG GTGTGCTCTC TGGTGATCAA GATACAGGTG ACCTGGTACT GCTTGATGTA 
TGTCCCCTGA CACTTGGTAT TGAAACTGTG GGAGGTGTCA TGACCAAATT GATTCCAAGG 
AACACTGTGG TGCCCACCAA GAAGTCTCAG ATCTTTTCTA CAGCCTCTGA TAATCAACCA 
ACTGTTACCA TCAAGGTCTA TGAAGGTGAA CGACCCCTGA CGAAAGACAA TCACCTTCTG 
GGAACTTTCG ACCTGACTGG AATTCCTCCT GCACCCCGTG GGGTCCCACA GATTGAAGTC 
ACCTTTGAGA TAGATGTGAA TGGCATTCTT CGAGTGACAG CTGAAGACAA AGGTACAGGC 
AACAAAAATA AGATCACAAT TACCAATGAC CAAAATCGCC TGACACCTGA AGAAATTGAA 
AGAATGGTCA ATGATGCTGA GAAGTTTGCT GAGGAAGACA AAAAGCTCAA GGAGCGCATC 
GACACAAGAA ATGAATTGGA AAGCTATGCC TACTCTCTTA AGAATCAGAT TGGAGATAAA 
GAAAAACTGG GAGGTAAACT TTCCTCAGAA GATAAGGAGA CCATGGAAAA AGCTGTAGAA 
GAAAAGATTG AGTGGCTGGA AAGTCACCAA GATGCTGACA TTGAAGATTT CAAAGCTAAA 
AAGAAGGAGC TAGAAGAAAT TGTTCAGCCA ATTATCAGCA AACTCTATGG AAGTGCAGGC 
CCTCCCCCAA CCAGTGAGGA GGAAGCAGCA GACAAAGATG AGTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001068616.1
CDS	122..2089
Translation	MKLSLVAAVLLLLLGTARAEEEDKKEDVGTVVGIDLGTTYSCVGVFKNGRVEIIANDQGNRITPSYVAFTPEGERLIGDAAKNQLTSNPENTVFDAKRLIGRTWNDPSVQQDIKFLPFKVVEKKTKPYIQVDVGGGQTKTFAPEEISAMVLTKMKETAEAYLGKKVTHAVVTVPAYFNDAQRQATKDAGTIAGLNVMRIINEPTAAAIAYGLDKREGEKNILVFDLGGGTFDVSLLTIDNGVFEVVATNGDTHLGGEDFDQRVMEHFIKLYKKKTGKDVRKDNRAVQKLRREVEKAKRALSSQHQARIEIESFYEGEDFSETLTRAKFEELNMDLFRSTMKPVQKVLEDSDLKKSDIDEIVLVGGSTRIPKIQQLVKEFFNGKEPSRGINPDEAVAYGAAVQAGVLSGDQDTGDLVLLDVCPLTLGIETVGGVMTKLIPRNTVVPTKKSQIFSTASDNQPTVTIKVYEGERPLTKDNHLLGTFDLTGIPPAPRGVPQIEVTFEIDVNGILRVTAEDKGTGNKNKITITNDQNRLTPEEIERMVNDAEKFAEEDKKLKERIDTRNELESYAYSLKNQIGDKEKLGGKLSSEDKETMEKAVEEKIEWLESHQDADIEDFKAKKKELEEIVQPIISKLYGSAGPPPTSEEEAADKDEL

Target ORF information:

RefSeq Version	NM_001075148.1
Organism	Bos taurus(cattle)
Definition	Bos taurus heat shock protein family A (Hsp70) member 5 (HSPA5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001075148.1

ORF Insert Sequence:

ATGAAGCTGT CCCTGGTGGC TGCGGTGCTG CTGCTGCTGC TTGGCACGGC ACGCGCGGAG 
GAGGAGGACA AGAAGGAGGA CGTGGGCACG GTGGTCGGCA TCGACCTGGG TACCACCTAC 
TCCTGCGTCG GGGTGTTCAA GAACGGCCGC GTGGAGATCA TCGCCAACGA TCAAGGCAAC 
CGCATCACGC CGTCTTATGT GGCTTTCACT CCTGAAGGGG AGCGTCTGAT CGGCGATGCA 
GCCAAGAACC AGCTCACCTC CAATCCGGAG AACACAGTCT TCGACGCCAA GCGACTCATC 
GGCCGGACGT GGAATGACCC GTCCGTGCAG CAGGACATCA AGTTCTTGCC TTTCAAGGTG 
GTTGAAAAGA AAACTAAACC ATACATTCAA GTTGATGTTG GAGGTGGGCA AACAAAGACA 
TTTGCTCCTG AAGAAATTTC TGCCATGGTT CTCACTAAAA TGAAGGAAAC TGCTGAGGCT 
TATTTGGGAA AGAAGGTTAC TCATGCAGTT GTTACTGTAC CAGCATATTT CAATGATGCC 
CAACGCCAGG CAACCAAAGA TGCTGGAACT ATTGCTGGAC TGAATGTCAT GAGGATCATC 
AATGAGCCTA CAGCAGCTGC TATTGCTTAT GGCCTGGATA AGAGGGAAGG GGAGAAGAAC 
ATCCTGGTGT TTGACCTGGG TGGTGGAACC TTTGATGTGT CTCTTCTCAC CATTGATAAT 
GGTGTCTTTG AAGTCGTGGC CACTAATGGA GATACTCATC TGGGTGGAGA AGACTTTGAC 
CAGCGTGTCA TGGAACACTT CATCAAACTC TACAAAAAGA AGACTGGCAA AGATGTTCGG 
AAGGACAACA GAGCTGTGCA GAAACTCCGG CGTGAGGTAG AAAAGGCCAA ACGGGCCCTG 
TCTTCCCAAC ATCAAGCAAG AATTGAAATT GAGTCCTTCT ATGAAGGAGA AGACTTCTCG 
GAGACCCTGA CTCGGGCTAA ATTTGAAGAG CTAAACATGG ACCTGTTCCG TTCCACCATG 
AAGCCTGTCC AGAAAGTGTT GGAAGATTCT GATTTAAAGA AGTCTGATAT TGATGAAATT 
GTTCTTGTTG GTGGCTCTAC TAGAATCCCA AAGATTCAAC AGCTGGTTAA AGAGTTTTTT 
AATGGCAAGG AGCCATCCCG TGGCATAAAC CCAGATGAGG CTGTGGCATA TGGTGCCGCT 
GTCCAGGCTG GTGTGCTCTC TGGTGATCAA GATACAGGTG ACCTGGTACT GCTTGATGTA 
TGTCCCCTGA CACTTGGTAT TGAAACTGTG GGAGGTGTCA TGACCAAATT GATTCCAAGG 
AACACTGTGG TGCCCACCAA GAAGTCTCAG ATCTTTTCTA CAGCCTCTGA TAATCAACCA 
ACTGTTACCA TCAAGGTCTA TGAAGGTGAA CGACCCCTGA CGAAAGACAA TCACCTTCTG 
GGAACTTTCG ACCTGACTGG AATTCCTCCT GCACCCCGTG GGGTCCCACA GATTGAAGTC 
ACCTTTGAGA TAGATGTGAA TGGCATTCTT CGAGTGACAG CTGAAGACAA AGGTACAGGC 
AACAAAAATA AGATCACAAT TACCAATGAC CAAAATCGCC TGACACCTGA AGAAATTGAA 
AGAATGGTCA ATGATGCTGA GAAGTTTGCT GAGGAAGACA AAAAGCTCAA GGAGCGCATC 
GACACAAGAA ATGAATTGGA AAGCTATGCC TACTCTCTTA AGAATCAGAT TGGAGATAAA 
GAAAAACTGG GAGGTAAACT TTCCTCAGAA GATAAGGAGA CCATGGAAAA AGCTGTAGAA 
GAAAAGATTG AGTGGCTGGA AAGTCACCAA GATGCTGACA TTGAAGATTT CAAAGCTAAA 
AAGAAGGAGC TAGAAGAAAT TGTTCAGCCA ATTATCAGCA AACTCTATGG AAGTGCAGGC 
CCTCCCCCAA CCAGTGAGGA GGAAGCAGCA GACAAAGATG AGTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HSPA5 ( XM_024998380.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024854148.1 Bos taurus heat shock protein family A (Hsp70) member 5 (HSPA5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa42804
Clone ID Related Accession (Same CDS sequence)	XM_024998380.1 , NM_001075148.1
Accession Version	XM_024998380.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1968bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	endoplasmic reticulum chaperone BiP isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGT CCCTGGTGGC TGCGGTGCTG CTGCTGCTGC TTGGCACGGC ACGCGCGGAG 
GAGGAGGACA AGAAGGAGGA CGTGGGCACG GTGGTCGGCA TCGACCTGGG TACCACCTAC 
TCCTGCGTCG GGGTGTTCAA GAACGGCCGC GTGGAGATCA TCGCCAACGA TCAAGGCAAC 
CGCATCACGC CGTCTTATGT GGCTTTCACT CCTGAAGGGG AGCGTCTGAT CGGCGATGCA 
GCCAAGAACC AGCTCACCTC CAATCCGGAG AACACAGTCT TCGACGCCAA GCGACTCATC 
GGCCGGACGT GGAATGACCC GTCCGTGCAG CAGGACATCA AGTTCTTGCC TTTCAAGGTG 
GTTGAAAAGA AAACTAAACC ATACATTCAA GTTGATGTTG GAGGTGGGCA AACAAAGACA 
TTTGCTCCTG AAGAAATTTC TGCCATGGTT CTCACTAAAA TGAAGGAAAC TGCTGAGGCT 
TATTTGGGAA AGAAGGTTAC TCATGCAGTT GTTACTGTAC CAGCATATTT CAATGATGCC 
CAACGCCAGG CAACCAAAGA TGCTGGAACT ATTGCTGGAC TGAATGTCAT GAGGATCATC 
AATGAGCCTA CAGCAGCTGC TATTGCTTAT GGCCTGGATA AGAGGGAAGG GGAGAAGAAC 
ATCCTGGTGT TTGACCTGGG TGGTGGAACC TTTGATGTGT CTCTTCTCAC CATTGATAAT 
GGTGTCTTTG AAGTCGTGGC CACTAATGGA GATACTCATC TGGGTGGAGA AGACTTTGAC 
CAGCGTGTCA TGGAACACTT CATCAAACTC TACAAAAAGA AGACTGGCAA AGATGTTCGG 
AAGGACAACA GAGCTGTGCA GAAACTCCGG CGTGAGGTAG AAAAGGCCAA ACGGGCCCTG 
TCTTCCCAAC ATCAAGCAAG AATTGAAATT GAGTCCTTCT ATGAAGGAGA AGACTTCTCG 
GAGACCCTGA CTCGGGCTAA ATTTGAAGAG CTAAACATGG ACCTGTTCCG TTCCACCATG 
AAGCCTGTCC AGAAAGTGTT GGAAGATTCT GATTTAAAGA AGTCTGATAT TGATGAAATT 
GTTCTTGTTG GTGGCTCTAC TAGAATCCCA AAGATTCAAC AGCTGGTTAA AGAGTTTTTT 
AATGGCAAGG AGCCATCCCG TGGCATAAAC CCAGATGAGG CTGTGGCATA TGGTGCCGCT 
GTCCAGGCTG GTGTGCTCTC TGGTGATCAA GATACAGGTG ACCTGGTACT GCTTGATGTA 
TGTCCCCTGA CACTTGGTAT TGAAACTGTG GGAGGTGTCA TGACCAAATT GATTCCAAGG 
AACACTGTGG TGCCCACCAA GAAGTCTCAG ATCTTTTCTA CAGCCTCTGA TAATCAACCA 
ACTGTTACCA TCAAGGTCTA TGAAGGTGAA CGACCCCTGA CGAAAGACAA TCACCTTCTG 
GGAACTTTCG ACCTGACTGG AATTCCTCCT GCACCCCGTG GGGTCCCACA GATTGAAGTC 
ACCTTTGAGA TAGATGTGAA TGGCATTCTT CGAGTGACAG CTGAAGACAA AGGTACAGGC 
AACAAAAATA AGATCACAAT TACCAATGAC CAAAATCGCC TGACACCTGA AGAAATTGAA 
AGAATGGTCA ATGATGCTGA GAAGTTTGCT GAGGAAGACA AAAAGCTCAA GGAGCGCATC 
GACACAAGAA ATGAATTGGA AAGCTATGCC TACTCTCTTA AGAATCAGAT TGGAGATAAA 
GAAAAACTGG GAGGTAAACT TTCCTCAGAA GATAAGGAGA CCATGGAAAA AGCTGTAGAA 
GAAAAGATTG AGTGGCTGGA AAGTCACCAA GATGCTGACA TTGAAGATTT CAAAGCTAAA 
AAGAAGGAGC TAGAAGAAAT TGTTCAGCCA ATTATCAGCA AACTCTATGG AAGTGCAGGC 
CCTCCCCCAA CCAGTGAGGA GGAAGCAGCA GACAAAGATG AGTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024854148.1
CDS	259..2226
Translation	MKLSLVAAVLLLLLGTARAEEEDKKEDVGTVVGIDLGTTYSCVGVFKNGRVEIIANDQGNRITPSYVAFTPEGERLIGDAAKNQLTSNPENTVFDAKRLIGRTWNDPSVQQDIKFLPFKVVEKKTKPYIQVDVGGGQTKTFAPEEISAMVLTKMKETAEAYLGKKVTHAVVTVPAYFNDAQRQATKDAGTIAGLNVMRIINEPTAAAIAYGLDKREGEKNILVFDLGGGTFDVSLLTIDNGVFEVVATNGDTHLGGEDFDQRVMEHFIKLYKKKTGKDVRKDNRAVQKLRREVEKAKRALSSQHQARIEIESFYEGEDFSETLTRAKFEELNMDLFRSTMKPVQKVLEDSDLKKSDIDEIVLVGGSTRIPKIQQLVKEFFNGKEPSRGINPDEAVAYGAAVQAGVLSGDQDTGDLVLLDVCPLTLGIETVGGVMTKLIPRNTVVPTKKSQIFSTASDNQPTVTIKVYEGERPLTKDNHLLGTFDLTGIPPAPRGVPQIEVTFEIDVNGILRVTAEDKGTGNKNKITITNDQNRLTPEEIERMVNDAEKFAEEDKKLKERIDTRNELESYAYSLKNQIGDKEKLGGKLSSEDKETMEKAVEEKIEWLESHQDADIEDFKAKKKELEEIVQPIISKLYGSAGPPPTSEEEAADKDEL

Target ORF information:

RefSeq Version	XM_024998380.1
Organism	Bos taurus(cattle)
Definition	Bos taurus heat shock protein family A (Hsp70) member 5 (HSPA5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024998380.1

ORF Insert Sequence:

ATGAAGCTGT CCCTGGTGGC TGCGGTGCTG CTGCTGCTGC TTGGCACGGC ACGCGCGGAG 
GAGGAGGACA AGAAGGAGGA CGTGGGCACG GTGGTCGGCA TCGACCTGGG TACCACCTAC 
TCCTGCGTCG GGGTGTTCAA GAACGGCCGC GTGGAGATCA TCGCCAACGA TCAAGGCAAC 
CGCATCACGC CGTCTTATGT GGCTTTCACT CCTGAAGGGG AGCGTCTGAT CGGCGATGCA 
GCCAAGAACC AGCTCACCTC CAATCCGGAG AACACAGTCT TCGACGCCAA GCGACTCATC 
GGCCGGACGT GGAATGACCC GTCCGTGCAG CAGGACATCA AGTTCTTGCC TTTCAAGGTG 
GTTGAAAAGA AAACTAAACC ATACATTCAA GTTGATGTTG GAGGTGGGCA AACAAAGACA 
TTTGCTCCTG AAGAAATTTC TGCCATGGTT CTCACTAAAA TGAAGGAAAC TGCTGAGGCT 
TATTTGGGAA AGAAGGTTAC TCATGCAGTT GTTACTGTAC CAGCATATTT CAATGATGCC 
CAACGCCAGG CAACCAAAGA TGCTGGAACT ATTGCTGGAC TGAATGTCAT GAGGATCATC 
AATGAGCCTA CAGCAGCTGC TATTGCTTAT GGCCTGGATA AGAGGGAAGG GGAGAAGAAC 
ATCCTGGTGT TTGACCTGGG TGGTGGAACC TTTGATGTGT CTCTTCTCAC CATTGATAAT 
GGTGTCTTTG AAGTCGTGGC CACTAATGGA GATACTCATC TGGGTGGAGA AGACTTTGAC 
CAGCGTGTCA TGGAACACTT CATCAAACTC TACAAAAAGA AGACTGGCAA AGATGTTCGG 
AAGGACAACA GAGCTGTGCA GAAACTCCGG CGTGAGGTAG AAAAGGCCAA ACGGGCCCTG 
TCTTCCCAAC ATCAAGCAAG AATTGAAATT GAGTCCTTCT ATGAAGGAGA AGACTTCTCG 
GAGACCCTGA CTCGGGCTAA ATTTGAAGAG CTAAACATGG ACCTGTTCCG TTCCACCATG 
AAGCCTGTCC AGAAAGTGTT GGAAGATTCT GATTTAAAGA AGTCTGATAT TGATGAAATT 
GTTCTTGTTG GTGGCTCTAC TAGAATCCCA AAGATTCAAC AGCTGGTTAA AGAGTTTTTT 
AATGGCAAGG AGCCATCCCG TGGCATAAAC CCAGATGAGG CTGTGGCATA TGGTGCCGCT 
GTCCAGGCTG GTGTGCTCTC TGGTGATCAA GATACAGGTG ACCTGGTACT GCTTGATGTA 
TGTCCCCTGA CACTTGGTAT TGAAACTGTG GGAGGTGTCA TGACCAAATT GATTCCAAGG 
AACACTGTGG TGCCCACCAA GAAGTCTCAG ATCTTTTCTA CAGCCTCTGA TAATCAACCA 
ACTGTTACCA TCAAGGTCTA TGAAGGTGAA CGACCCCTGA CGAAAGACAA TCACCTTCTG 
GGAACTTTCG ACCTGACTGG AATTCCTCCT GCACCCCGTG GGGTCCCACA GATTGAAGTC 
ACCTTTGAGA TAGATGTGAA TGGCATTCTT CGAGTGACAG CTGAAGACAA AGGTACAGGC 
AACAAAAATA AGATCACAAT TACCAATGAC CAAAATCGCC TGACACCTGA AGAAATTGAA 
AGAATGGTCA ATGATGCTGA GAAGTTTGCT GAGGAAGACA AAAAGCTCAA GGAGCGCATC 
GACACAAGAA ATGAATTGGA AAGCTATGCC TACTCTCTTA AGAATCAGAT TGGAGATAAA 
GAAAAACTGG GAGGTAAACT TTCCTCAGAA GATAAGGAGA CCATGGAAAA AGCTGTAGAA 
GAAAAGATTG AGTGGCTGGA AAGTCACCAA GATGCTGACA TTGAAGATTT CAAAGCTAAA 
AAGAAGGAGC TAGAAGAAAT TGTTCAGCCA ATTATCAGCA AACTCTATGG AAGTGCAGGC 
CCTCCCCCAA CCAGTGAGGA GGAAGCAGCA GACAAAGATG AGTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

AB5 subtilase cytotoxin inactivates the endoplasmic reticulum chaperone BiP.
Nature443(7111)548-52(2006 Oct)
Paton AW,Beddoe T,Thorpe CM,Whisstock JC,Wilce MC,Rossjohn J,Talbot UM,Paton JC

Characterization of 954 bovine full-CDS cDNA sequences.
BMC genomics6166(2005 Nov)
Harhay GP,Sonstegard TS,Keele JW,Heaton MP,Clawson ML,Snelling WM,Wiedmann RT,Van Tassell CP,Smith TP

Localization of the chaperone proteins GRP78 and HSP60 on the luminal surface of bovine oviduct epithelial cells and their association with spermatozoa.
Biology of reproduction71(6)1879-89(2004 Dec)
Boilard M,Reyes-Moreno C,Lachance C,Massicotte L,Bailey JL,Sirard MA,Leclerc P

Monitoring gene expression changes in bovine oviduct epithelial cells during the oestrous cycle.
Journal of molecular endocrinology32(2)449-66(2004 Apr)
Bauersachs S,Rehfeld S,Ulbrich SE,Mallok S,Prelle K,Wenigerkind H,Einspanier R,Blum H,Wolf E

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HSPA5 cDNA ORF clone, Bos taurus(cattle)

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ORF Insert Sequence:

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Target ORF information:

ORF Insert Sequence:

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