CACNA2D3A cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	CACNA2D3A
Entrez Gene ID	100535196
Full Name	calcium channel, voltage-dependent, alpha 2/delta subunit 3a
General protein information	Preferred Names calcium channel, voltage-dependent, alpha 2/delta subunit 3a Names voltage-dependent calcium channel subunit alpha-2/delta-3 calcium channel, voltage-dependent, alpha 2/delta subunit 3
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021478512.1	XP_021334187.1	voltage-dependent calcium channel subunit alpha-2/delta-3 isoform X2
XM_009304342.3	XP_009302617.1	voltage-dependent calcium channel subunit alpha-2/delta-3 isoform X1
XM_021478513.1	XP_021334188.1	voltage-dependent calcium channel subunit alpha-2/delta-3 isoform X3
XM_009304342.2	XP_009302617.1	voltage-dependent calcium channel subunit alpha-2/delta-3

Pathways from BioSystems

Homologies

Homo sapiens (human)	CACNA2D3	NP_060868.2
Pan troglodytes (chimpanzee)	CACNA2D3	XP_516539.3
Canis lupus familiaris (dog)	CACNA2D3	XP_005633241.1
Mus musculus (house mouse)	Cacna2d3	NP_033915.1
Rattus norvegicus (Norway rat)	Cacna2d3	NP_783185.1
Bos taurus (cattle)	CACNA2D3	NP_001193680.1
Danio rerio (zebrafish)	cacna2d3	XP_005172562.1
Drosophila melanogaster (fruit fly)	stj	NP_001246303.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005490	XP_315491.4
Gallus gallus (chicken)	CACNA2D3	XP_414338.2
Xenopus tropicalis (tropical clawed frog)	cacna2d3	XP_004914404.1

Gene Ontology

The following CACNA2D3A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CACNA2D3A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa50128	XM_021478513.1 Latest version!	Danio rerio calcium channel, voltage-dependent, alpha 2/delta subunit 3a (cacna2d3a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
ODa50127	XM_021478512.1 Latest version!	Danio rerio calcium channel, voltage-dependent, alpha 2/delta subunit 3a (cacna2d3a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
ODa50126	XM_009304342.3 Latest version!	Danio rerio calcium channel, voltage-dependent, alpha 2/delta subunit 3a (cacna2d3a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
ODa50126	XM_009304342.2	Danio rerio calcium channel, voltage-dependent, alpha 2/delta subunit 3a (cacna2d3a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CACNA2D3A ( XM_021478513.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021334188.1 Danio rerio calcium channel, voltage-dependent, alpha 2/delta subunit 3a (cacna2d3a), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa50128
Clone ID Related Accession (Same CDS sequence)	XM_021478513.1
Accession Version	XM_021478513.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2988bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent calcium channel subunit alpha-2/delta-3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGA GGTTTCACAA GAAAGCTGAA GCCATAAAGC GTTTGGTGGA AGCAGCAGAA 
GAAGCACACC TGAACCACGA GGAGGATCCT GACCTACAGT ACGAGTATTT TAATGCAGTG 
TTGATCAATG AGTTGGACGA GGAGGGCCGC AGCGTGGAGC TGGGTGGAGA ATTCATCCTT 
CAGCCGAACG AACATTTCAA TAATCTGTCA GTAAACCTCA GCCTCAGTGT CGTCCAGGTG 
CCAACTAACA TGTACAACAA AGACTCAGAC ATCGTGAACG GGGTTTACTG GTCAGAGGCT 
CTCAACACAG TGTTTGTGGA CAACTTTAAA AGAGACCCTT CTCTCCTATG GCAGTATTTT 
GGCAGCGCTC AAGGTTTCTT CAGACAGTAT CCAGGAATGA AGTGGACGCC TGATGAACAC 
GGAGTTCTAG AGTTTGACTG TCGAAATCGG AAATGGTATA TCCAGGCCGC TACGTCTCCT 
AAAGATGTGG TGATATTGGT GGATGTGAGC GGCAGTATGA AGGGTTTACG GCTCACCATC 
GCTAGACAGA CCGTCTCCTC TATACTGGAC ACACTCGGAG ACGACGACTT CTTCAACGTT 
ATTGCTTACA ACGAGGATCT GCATTACGTG GAGCCGTGTC TGAACGGAAC ATTAGTTCAG 
GCTGACATTA CCAACAAAGA TCATTTCCGC CAGCGTCTCG ATAAGCTCCT CGCTAAAGGG 
ATCGGCATGC TGGACGTGGC TCTGACAGAG GCCTTTGAGC TGCTCAGCAA TTTTAATCAG 
ACAGGCAGAG GCAGTGAGTG CAGTCAGGCT ATAATGCTGG TGACAGACGG CGCAGTGGAG 
ACATATGATG CTGTGTTCGC CGTCTACAAC TGGCCTGACA GGAAGGTGCG TGTTTTCCCT 
TACCTGATTG GTCGAGAGTC TGCCTTCGCT GATAACCTGA AGTGGATGGC CTGCGCTAAT 
AAAGGTTACT TCACCCAGAT CTCCACGCTG GCGGACGTAC AGGAGAACGT GATGAAGTAT 
CTGCATGTTC TCAGTCGGCC GAAAGTCATC GATCGAGAGC ATGACATGGT GTGGACTGAA 
GCCTACATCG ACAACACTCG TTCGCAGGCT CAGAAGCTGG AAGACGCTCA GGGTCCAGTG 
CTCATGACTA CCGTGGCCAT GCCTGTGTTC AGCACCAAAA ACGAGACCAG GAATAAAGGG 
ATTCTTCTTG GAGTGGTCGG CACAGATGTA CCAGTGTCAG AAATCCTGAA GGCCATCCCC 
AAATACAAGC TGGGAATCCA TGGGTATGCC TTTGCCATCA CCAACAACGG ATACATCCTC 
ACCCATCCGG ACCTGCAGCC GCTGTATGAA GAAGGGAAAA AGAGGAGAAA GTCCAGTTAC 
AGCAGCGCAG ATCTGTCCGA GGTGGAGTGG GAGGACAAGG AGGACGAGCT GCGTAACGCT 
ATGGTGAACA GACAGACGGG AAGCTTCTCT ATGAAAGTCA AGAAAACTGT GGATAAAGGG 
AAGCGGGTGC TTGTTCTTCA CAATGATTAC TACTACACAG ACATTAAAGG AACGCCCTTC 
AGCTTAGGAG TGGCTCTGTC TGGAGGTCAC GGCAAATATG TCTTCAGAGG AAATATCTCT 
GTTGAAGAAG GGCTGCATGA TTTGGAGCAT CCAGACGTTG CATTAGCAGA TGAATGGACA 
TACTGTAACA CAGAGGAACA TCCAGACCAT CCCGTGCTCT CTCAGATTGA AGCCATTAAG 
CTGTATTTCA ATGGAGAAGA GCATCTGAAG TGTGATCAGG AGCTGGTGCA GGAGGTGTTG 
TTTGATGCGG TGGTCACGGC ACCACTGGAG GCGTACTGGA CCAGCCTCGC TCTCAATAAA 
TCCGAAAACT CTGATAAAGG TGTGGAGATC TCTTTCCTCG CCACTCGAAC TGGATTATCA 
CGCATCAACC TTTTTGTGAC GCCAGATCAA CTGAGCAATC AAGGCCTCCT GACAGGAGAG 
GATAAAGAGG TGCAGTTCAG CGCTGATCAT TTCCCTCTGT GGTACAGAAG AGCTGCTGAA 
CAGGTGCCCG GCACATTTGT CTACAGCCTT CCGTATGACG CAGCCTCTGA CAATAAGAGT 
GTGGTCTTGG CCAGCAGCGC CATCCATCTG CTGGATGAGA GAGAGTCACC CATCGCTGCA 
GCTGTAGGTA TTCAGATGAA GCTAGAGTTC TTTCAGAGGA AGTTCTGGAC CGCCAGCAGA 
CAGTGTGCGG CGCTTGATGG AAAGTGTTCA ATCAGCTGTG ATAACGATGA CATCAGCTGC 
TACCTCATAG ATAATAATGG ATTTATTATT GTGGCCGAGG ATCAGTCTTT GACTGGTGTG 
TTTTTTGGAG AGCCTGAAGG AGCGGTGATG GGCAAACTGG TGTCAATGGG CTCATTTAAG 
AGGGTGAATC TGTATGACTA CCGGGCCATC TGTAGGGTGT ATGCGCAGAC CAGCGACACA 
GCACACACAC TTCTACACCC CTACTTTGCT CTGTTCGCAG CTATCCGATG GCTCCTGACG 
GAGTTTGTCA TATTCCTGGT TGAGTTTAAC CTGTACAGCT GGTGGCACTC TGACCTCACC 
ATTACAGCTC ACAGAGGAGG CAGAACTCTG CTGGTTCCCT GCGACACCGA GTATCCAGCC 
TTCGTGTCCG AGCGCACCAT TAAAGAGACT GTGGGCAATA TTGACTGTGA AAGCTGTGTG 
AAATCGTTTG TGATTCAGCA GATTCCCAGC AGTAATCTCT TCATGGTGGT CATTGATAAC 
ACATGTGACT GCAGCGACTT CGGACCCGTG ACCATGGAGC CTGTCGAAAT CATGTATAAT 
GAGTCTCTGA AATGTGAGCG GCTGAAGTTT CAGAAGGACA GAAGACGTCC AGACACGTGC 
CATCCATTCC ATCATGAGGA GAACTCAATG GAGTGCGGTG GTTCAGTCAG TCTGTCTCCA 
TCCTCTCCAG CTCTTCTAGT GCTAATAATC TCCATCTTCA GCAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021334188.1
CDS	229..3216
Translation	MEQRFHKKAEAIKRLVEAAEEAHLNHEEDPDLQYEYFNAVLINELDEEGRSVELGGEFILQPNEHFNNLSVNLSLSVVQVPTNMYNKDSDIVNGVYWSEALNTVFVDNFKRDPSLLWQYFGSAQGFFRQYPGMKWTPDEHGVLEFDCRNRKWYIQAATSPKDVVILVDVSGSMKGLRLTIARQTVSSILDTLGDDDFFNVIAYNEDLHYVEPCLNGTLVQADITNKDHFRQRLDKLLAKGIGMLDVALTEAFELLSNFNQTGRGSECSQAIMLVTDGAVETYDAVFAVYNWPDRKVRVFPYLIGRESAFADNLKWMACANKGYFTQISTLADVQENVMKYLHVLSRPKVIDREHDMVWTEAYIDNTRSQAQKLEDAQGPVLMTTVAMPVFSTKNETRNKGILLGVVGTDVPVSEILKAIPKYKLGIHGYAFAITNNGYILTHPDLQPLYEEGKKRRKSSYSSADLSEVEWEDKEDELRNAMVNRQTGSFSMKVKKTVDKGKRVLVLHNDYYYTDIKGTPFSLGVALSGGHGKYVFRGNISVEEGLHDLEHPDVALADEWTYCNTEEHPDHPVLSQIEAIKLYFNGEEHLKCDQELVQEVLFDAVVTAPLEAYWTSLALNKSENSDKGVEISFLATRTGLSRINLFVTPDQLSNQGLLTGEDKEVQFSADHFPLWYRRAAEQVPGTFVYSLPYDAASDNKSVVLASSAIHLLDERESPIAAAVGIQMKLEFFQRKFWTASRQCAALDGKCSISCDNDDISCYLIDNNGFIIVAEDQSLTGVFFGEPEGAVMGKLVSMGSFKRVNLYDYRAICRVYAQTSDTAHTLLHPYFALFAAIRWLLTEFVIFLVEFNLYSWWHSDLTITAHRGGRTLLVPCDTEYPAFVSERTIKETVGNIDCESCVKSFVIQQIPSSNLFMVVIDNTCDCSDFGPVTMEPVEIMYNESLKCERLKFQKDRRRPDTCHPFHHEENSMECGGSVSLSPSSPALLVLIISIFSR

Target ORF information:

RefSeq Version	XM_021478513.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, alpha 2/delta subunit 3a (cacna2d3a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021478513.1

ORF Insert Sequence:

ATGGAGCAGA GGTTTCACAA GAAAGCTGAA GCCATAAAGC GTTTGGTGGA AGCAGCAGAA 
GAAGCACACC TGAACCACGA GGAGGATCCT GACCTACAGT ACGAGTATTT TAATGCAGTG 
TTGATCAATG AGTTGGACGA GGAGGGCCGC AGCGTGGAGC TGGGTGGAGA ATTCATCCTT 
CAGCCGAACG AACATTTCAA TAATCTGTCA GTAAACCTCA GCCTCAGTGT CGTCCAGGTG 
CCAACTAACA TGTACAACAA AGACTCAGAC ATCGTGAACG GGGTTTACTG GTCAGAGGCT 
CTCAACACAG TGTTTGTGGA CAACTTTAAA AGAGACCCTT CTCTCCTATG GCAGTATTTT 
GGCAGCGCTC AAGGTTTCTT CAGACAGTAT CCAGGAATGA AGTGGACGCC TGATGAACAC 
GGAGTTCTAG AGTTTGACTG TCGAAATCGG AAATGGTATA TCCAGGCCGC TACGTCTCCT 
AAAGATGTGG TGATATTGGT GGATGTGAGC GGCAGTATGA AGGGTTTACG GCTCACCATC 
GCTAGACAGA CCGTCTCCTC TATACTGGAC ACACTCGGAG ACGACGACTT CTTCAACGTT 
ATTGCTTACA ACGAGGATCT GCATTACGTG GAGCCGTGTC TGAACGGAAC ATTAGTTCAG 
GCTGACATTA CCAACAAAGA TCATTTCCGC CAGCGTCTCG ATAAGCTCCT CGCTAAAGGG 
ATCGGCATGC TGGACGTGGC TCTGACAGAG GCCTTTGAGC TGCTCAGCAA TTTTAATCAG 
ACAGGCAGAG GCAGTGAGTG CAGTCAGGCT ATAATGCTGG TGACAGACGG CGCAGTGGAG 
ACATATGATG CTGTGTTCGC CGTCTACAAC TGGCCTGACA GGAAGGTGCG TGTTTTCCCT 
TACCTGATTG GTCGAGAGTC TGCCTTCGCT GATAACCTGA AGTGGATGGC CTGCGCTAAT 
AAAGGTTACT TCACCCAGAT CTCCACGCTG GCGGACGTAC AGGAGAACGT GATGAAGTAT 
CTGCATGTTC TCAGTCGGCC GAAAGTCATC GATCGAGAGC ATGACATGGT GTGGACTGAA 
GCCTACATCG ACAACACTCG TTCGCAGGCT CAGAAGCTGG AAGACGCTCA GGGTCCAGTG 
CTCATGACTA CCGTGGCCAT GCCTGTGTTC AGCACCAAAA ACGAGACCAG GAATAAAGGG 
ATTCTTCTTG GAGTGGTCGG CACAGATGTA CCAGTGTCAG AAATCCTGAA GGCCATCCCC 
AAATACAAGC TGGGAATCCA TGGGTATGCC TTTGCCATCA CCAACAACGG ATACATCCTC 
ACCCATCCGG ACCTGCAGCC GCTGTATGAA GAAGGGAAAA AGAGGAGAAA GTCCAGTTAC 
AGCAGCGCAG ATCTGTCCGA GGTGGAGTGG GAGGACAAGG AGGACGAGCT GCGTAACGCT 
ATGGTGAACA GACAGACGGG AAGCTTCTCT ATGAAAGTCA AGAAAACTGT GGATAAAGGG 
AAGCGGGTGC TTGTTCTTCA CAATGATTAC TACTACACAG ACATTAAAGG AACGCCCTTC 
AGCTTAGGAG TGGCTCTGTC TGGAGGTCAC GGCAAATATG TCTTCAGAGG AAATATCTCT 
GTTGAAGAAG GGCTGCATGA TTTGGAGCAT CCAGACGTTG CATTAGCAGA TGAATGGACA 
TACTGTAACA CAGAGGAACA TCCAGACCAT CCCGTGCTCT CTCAGATTGA AGCCATTAAG 
CTGTATTTCA ATGGAGAAGA GCATCTGAAG TGTGATCAGG AGCTGGTGCA GGAGGTGTTG 
TTTGATGCGG TGGTCACGGC ACCACTGGAG GCGTACTGGA CCAGCCTCGC TCTCAATAAA 
TCCGAAAACT CTGATAAAGG TGTGGAGATC TCTTTCCTCG CCACTCGAAC TGGATTATCA 
CGCATCAACC TTTTTGTGAC GCCAGATCAA CTGAGCAATC AAGGCCTCCT GACAGGAGAG 
GATAAAGAGG TGCAGTTCAG CGCTGATCAT TTCCCTCTGT GGTACAGAAG AGCTGCTGAA 
CAGGTGCCCG GCACATTTGT CTACAGCCTT CCGTATGACG CAGCCTCTGA CAATAAGAGT 
GTGGTCTTGG CCAGCAGCGC CATCCATCTG CTGGATGAGA GAGAGTCACC CATCGCTGCA 
GCTGTAGGTA TTCAGATGAA GCTAGAGTTC TTTCAGAGGA AGTTCTGGAC CGCCAGCAGA 
CAGTGTGCGG CGCTTGATGG AAAGTGTTCA ATCAGCTGTG ATAACGATGA CATCAGCTGC 
TACCTCATAG ATAATAATGG ATTTATTATT GTGGCCGAGG ATCAGTCTTT GACTGGTGTG 
TTTTTTGGAG AGCCTGAAGG AGCGGTGATG GGCAAACTGG TGTCAATGGG CTCATTTAAG 
AGGGTGAATC TGTATGACTA CCGGGCCATC TGTAGGGTGT ATGCGCAGAC CAGCGACACA 
GCACACACAC TTCTACACCC CTACTTTGCT CTGTTCGCAG CTATCCGATG GCTCCTGACG 
GAGTTTGTCA TATTCCTGGT TGAGTTTAAC CTGTACAGCT GGTGGCACTC TGACCTCACC 
ATTACAGCTC ACAGAGGAGG CAGAACTCTG CTGGTTCCCT GCGACACCGA GTATCCAGCC 
TTCGTGTCCG AGCGCACCAT TAAAGAGACT GTGGGCAATA TTGACTGTGA AAGCTGTGTG 
AAATCGTTTG TGATTCAGCA GATTCCCAGC AGTAATCTCT TCATGGTGGT CATTGATAAC 
ACATGTGACT GCAGCGACTT CGGACCCGTG ACCATGGAGC CTGTCGAAAT CATGTATAAT 
GAGTCTCTGA AATGTGAGCG GCTGAAGTTT CAGAAGGACA GAAGACGTCC AGACACGTGC 
CATCCATTCC ATCATGAGGA GAACTCAATG GAGTGCGGTG GTTCAGTCAG TCTGTCTCCA 
TCCTCTCCAG CTCTTCTAGT GCTAATAATC TCCATCTTCA GCAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA2D3A ( XM_021478512.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021334187.1 Danio rerio calcium channel, voltage-dependent, alpha 2/delta subunit 3a (cacna2d3a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa50127
Clone ID Related Accession (Same CDS sequence)	XM_021478512.1
Accession Version	XM_021478512.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent calcium channel subunit alpha-2/delta-3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTGTG TTTTCTGTCG GCTGTTTGTC CTCCTCCTGT GGATTATTAA TGATTCAGGA 
ATATGTGCGT CTCATCAAGG GATCCCCCTG TCAGTTGTGA AACTTTGGGC TTCGGCGTTT 
GGCGGAGAGA TAAAATCCAT CGCAGCTAAA TATTCAGGCT CTCAGCTGCT GCAAAAGAAG 
TATAAGGAGT TTGAGCGGTC GGTGAGGGTG GAGGAGATCG ATGGCATCAA ACTGGTGAAG 
AAACTCGCTG CCAAAATGGA GCAGAGGTTT CACAAGAAAG CTGAAGCCAT AAAGCGTTTG 
GTGGAAGCAG CAGAAGAAGC ACACCTGAAC CACGAGGAGG ATCCTGACCT ACAGTACGAG 
TATTTTAATG CAGTGTTGAT CAATGAGTTG GACGAGGAGG GCCGCAGCGT GGAGCTGGGT 
GGAGAATTCA TCCTTCAGCC GAACGAACAT TTCAATAATC TGTCAGTAAA CCTCAGCCTC 
AGTGTCGTCC AGGTGCCAAC TAACATGTAC AACAAAGACT CAGACATCGT GAACGGGGTT 
TACTGGTCAG AGGCTCTCAA CACAGTGTTT GTGGACAACT TTAAAAGAGA CCCTTCTCTC 
CTATGGCAGT ATTTTGGCAG CGCTCAAGGT TTCTTCAGAC AGTATCCAGG AATGAAGTGG 
ACGCCTGATG AACACGGAGT TCTAGAGTTT GACTGTCGAA ATCGGAAATG GTATATCCAG 
GCCGCTACGT CTCCTAAAGA TGTGGTGATA TTGGTGGATG TGAGCGGCAG TATGAAGGGT 
TTACGGCTCA CCATCGCTAG ACAGACCGTC TCCTCTATAC TGGACACACT CGGAGACGAC 
GACTTCTTCA ACGTTATTGC TTACAACGAG GATCTGCATT ACGTGGAGCC GTGTCTGAAC 
GGAACATTAG TTCAGGCTGA CATTACCAAC AAAGATCATT TCCGCCAGCG TCTCGATAAG 
CTCCTCGCTA AAGGGATCGG CATGCTGGAC GTGGCTCTGA CAGAGGCCTT TGAGCTGCTC 
AGCAATTTTA ATCAGACAGG CAGAGGCAGT GAGTGCAGTC AGGCTATAAT GCTGGTGACA 
GACGGCGCAG TGGAGACATA TGATGCTGTG TTCGCCGTCT ACAACTGGCC TGACAGGAAG 
GTGCGTGTTT TCCCTTACCT GATTGGTCGA GAGTCTGCCT TCGCTGATAA CCTGAAGTGG 
ATGGCCTGCG CTAATAAAGG TTACTTCACC CAGATCTCCA CGCTGGCGGA CGTACAGGAG 
AACGTGATGA AGTATCTGCA TGTTCTCAGT CGGCCGAAAG TCATCGATCG AGAGCATGAC 
ATGGTGTGGA CTGAAGCCTA CATCGACAAC ACTCTGGAAG ACGCTCAGGG TCCAGTGCTC 
ATGACTACCG TGGCCATGCC TGTGTTCAGC ACCAAAAACG AGACCAGGAA TAAAGGGATT 
CTTCTTGGAG TGGTCGGCAC AGATGTACCA GTGTCAGAAA TCCTGAAGGC CATCCCCAAA 
TACAAGCTGG GAATCCATGG GTATGCCTTT GCCATCACCA ACAACGGATA CATCCTCACC 
CATCCGGACC TGCAGCCGCT GTATGAAGAA GGGAAAAAGA GGAGAAAGTC CAGTTACAGC 
AGCGCAGATC TGTCCGAGGT GGAGTGGGAG GACAAGGAGG ACGAGCTGCG TAACGCTATG 
GTGAACAGAC AGACGGGAAG CTTCTCTATG AAAGTCAAGA AAACTGTGGA TAAAGGGAAG 
CGGGTGCTTG TTCTTCACAA TGATTACTAC TACACAGACA TTAAAGGAAC GCCCTTCAGC 
TTAGGAGTGG CTCTGTCTGG AGGTCACGGC AAATATGTCT TCAGAGGAAA TATCTCTGTT 
GAAGAAGGGC TGCATGATTT GGAGCATCCA GACGTTGCAT TAGCAGATGA ATGGACATAC 
TGTAACACAG AGGAACATCC AGACCATCCC GTGCTCTCTC AGATTGAAGC CATTAAGCTG 
TATTTCAATG GAGAAGAGCA TCTGAAGTGT GATCAGGAGC TGGTGCAGGA GGTGTTGTTT 
GATGCGGTGG TCACGGCACC ACTGGAGGCG TACTGGACCA GCCTCGCTCT CAATAAATCC 
GAAAACTCTG ATAAAGGTGT GGAGATCTCT TTCCTCGCCA CTCGAACTGG ATTATCACGC 
ATCAACCTTT TTGTGACGCC AGATCAACTG AGCAATCAAG GCCTCCTGAC AGGAGAGGAT 
AAAGAGGTGC AGTTCAGCGC TGATCATTTC CCTCTGTGGT ACAGAAGAGC TGCTGAACAG 
GTGCCCGGCA CATTTGTCTA CAGCCTTCCG TATGACGCAG CCTCTGACAA TAAGAGTGTG 
GTCTTGGCCA GCAGCGCCAT CCATCTGCTG GATGAGAGAG AGTCACCCAT CGCTGCAGCT 
GTAGGTATTC AGATGAAGCT AGAGTTCTTT CAGAGGAAGT TCTGGACCGC CAGCAGACAG 
TGTGCGGCGC TTGATGGAAA GTGTTCAATC AGCTGTGATA ACGATGACAT CAGCTGCTAC 
CTCATAGATA ATAATGGATT TATTATTGTG GCCGAGGATC AGTCTTTGAC TGGTGTGTTT 
TTTGGAGAGC CTGAAGGAGC GGTGATGGGC AAACTGGTGT CAATGGGCTC ATTTAAGAGG 
GTGAATCTGT ATGACTACCG GGCCATCTGT AGGGTGTATG CGCAGACCAG CGACACAGCA 
CACACACTTC TACACCCCTA CTTTGCTCTG TTCGCAGCTA TCCGATGGCT CCTGACGGAG 
TTTGTCATAT TCCTGGTTGA GTTTAACCTG TACAGCTGGT GGCACTCTGA CCTCACCATT 
ACAGCTCACA GAGGAGGCAG AACTCTGCTG GTTCCCTGCG ACACCGAGTA TCCAGCCTTC 
GTGTCCGAGC GCACCATTAA AGAGACTGTG GGCAATATTG ACTGTGAAAG CTGTGTGAAA 
TCGTTTGTGA TTCAGCAGAT TCCCAGCAGT AATCTCTTCA TGGTGGTCAT TGATAACACA 
TGTGACTGCA GCGACTTCGG ACCCGTGACC ATGGAGCCTG TCGAAATCAT GTATAATGAG 
TCTCTGAAAT GTGAGCGGCT GAAGTTTCAG AAGGACAGAA GACGTCCAGA CACGTGCCAT 
CCATTCCATC ATGAGGAGAA CTCAATGGAG TGCGGTGGTT CAGTCAGTCT GTCTCCATCC 
TCTCCAGCTC TTCTAGTGCT AATAATCTCC ATCTTCAGCA GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021334187.1
CDS	184..3408
Translation	MKCVFCRLFVLLLWIINDSGICASHQGIPLSVVKLWASAFGGEIKSIAAKYSGSQLLQKKYKEFERSVRVEEIDGIKLVKKLAAKMEQRFHKKAEAIKRLVEAAEEAHLNHEEDPDLQYEYFNAVLINELDEEGRSVELGGEFILQPNEHFNNLSVNLSLSVVQVPTNMYNKDSDIVNGVYWSEALNTVFVDNFKRDPSLLWQYFGSAQGFFRQYPGMKWTPDEHGVLEFDCRNRKWYIQAATSPKDVVILVDVSGSMKGLRLTIARQTVSSILDTLGDDDFFNVIAYNEDLHYVEPCLNGTLVQADITNKDHFRQRLDKLLAKGIGMLDVALTEAFELLSNFNQTGRGSECSQAIMLVTDGAVETYDAVFAVYNWPDRKVRVFPYLIGRESAFADNLKWMACANKGYFTQISTLADVQENVMKYLHVLSRPKVIDREHDMVWTEAYIDNTLEDAQGPVLMTTVAMPVFSTKNETRNKGILLGVVGTDVPVSEILKAIPKYKLGIHGYAFAITNNGYILTHPDLQPLYEEGKKRRKSSYSSADLSEVEWEDKEDELRNAMVNRQTGSFSMKVKKTVDKGKRVLVLHNDYYYTDIKGTPFSLGVALSGGHGKYVFRGNISVEEGLHDLEHPDVALADEWTYCNTEEHPDHPVLSQIEAIKLYFNGEEHLKCDQELVQEVLFDAVVTAPLEAYWTSLALNKSENSDKGVEISFLATRTGLSRINLFVTPDQLSNQGLLTGEDKEVQFSADHFPLWYRRAAEQVPGTFVYSLPYDAASDNKSVVLASSAIHLLDERESPIAAAVGIQMKLEFFQRKFWTASRQCAALDGKCSISCDNDDISCYLIDNNGFIIVAEDQSLTGVFFGEPEGAVMGKLVSMGSFKRVNLYDYRAICRVYAQTSDTAHTLLHPYFALFAAIRWLLTEFVIFLVEFNLYSWWHSDLTITAHRGGRTLLVPCDTEYPAFVSERTIKETVGNIDCESCVKSFVIQQIPSSNLFMVVIDNTCDCSDFGPVTMEPVEIMYNESLKCERLKFQKDRRRPDTCHPFHHEENSMECGGSVSLSPSSPALLVLIISIFSR

Target ORF information:

RefSeq Version	XM_021478512.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, alpha 2/delta subunit 3a (cacna2d3a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021478512.1

ORF Insert Sequence:

ATGAAGTGTG TTTTCTGTCG GCTGTTTGTC CTCCTCCTGT GGATTATTAA TGATTCAGGA 
ATATGTGCGT CTCATCAAGG GATCCCCCTG TCAGTTGTGA AACTTTGGGC TTCGGCGTTT 
GGCGGAGAGA TAAAATCCAT CGCAGCTAAA TATTCAGGCT CTCAGCTGCT GCAAAAGAAG 
TATAAGGAGT TTGAGCGGTC GGTGAGGGTG GAGGAGATCG ATGGCATCAA ACTGGTGAAG 
AAACTCGCTG CCAAAATGGA GCAGAGGTTT CACAAGAAAG CTGAAGCCAT AAAGCGTTTG 
GTGGAAGCAG CAGAAGAAGC ACACCTGAAC CACGAGGAGG ATCCTGACCT ACAGTACGAG 
TATTTTAATG CAGTGTTGAT CAATGAGTTG GACGAGGAGG GCCGCAGCGT GGAGCTGGGT 
GGAGAATTCA TCCTTCAGCC GAACGAACAT TTCAATAATC TGTCAGTAAA CCTCAGCCTC 
AGTGTCGTCC AGGTGCCAAC TAACATGTAC AACAAAGACT CAGACATCGT GAACGGGGTT 
TACTGGTCAG AGGCTCTCAA CACAGTGTTT GTGGACAACT TTAAAAGAGA CCCTTCTCTC 
CTATGGCAGT ATTTTGGCAG CGCTCAAGGT TTCTTCAGAC AGTATCCAGG AATGAAGTGG 
ACGCCTGATG AACACGGAGT TCTAGAGTTT GACTGTCGAA ATCGGAAATG GTATATCCAG 
GCCGCTACGT CTCCTAAAGA TGTGGTGATA TTGGTGGATG TGAGCGGCAG TATGAAGGGT 
TTACGGCTCA CCATCGCTAG ACAGACCGTC TCCTCTATAC TGGACACACT CGGAGACGAC 
GACTTCTTCA ACGTTATTGC TTACAACGAG GATCTGCATT ACGTGGAGCC GTGTCTGAAC 
GGAACATTAG TTCAGGCTGA CATTACCAAC AAAGATCATT TCCGCCAGCG TCTCGATAAG 
CTCCTCGCTA AAGGGATCGG CATGCTGGAC GTGGCTCTGA CAGAGGCCTT TGAGCTGCTC 
AGCAATTTTA ATCAGACAGG CAGAGGCAGT GAGTGCAGTC AGGCTATAAT GCTGGTGACA 
GACGGCGCAG TGGAGACATA TGATGCTGTG TTCGCCGTCT ACAACTGGCC TGACAGGAAG 
GTGCGTGTTT TCCCTTACCT GATTGGTCGA GAGTCTGCCT TCGCTGATAA CCTGAAGTGG 
ATGGCCTGCG CTAATAAAGG TTACTTCACC CAGATCTCCA CGCTGGCGGA CGTACAGGAG 
AACGTGATGA AGTATCTGCA TGTTCTCAGT CGGCCGAAAG TCATCGATCG AGAGCATGAC 
ATGGTGTGGA CTGAAGCCTA CATCGACAAC ACTCTGGAAG ACGCTCAGGG TCCAGTGCTC 
ATGACTACCG TGGCCATGCC TGTGTTCAGC ACCAAAAACG AGACCAGGAA TAAAGGGATT 
CTTCTTGGAG TGGTCGGCAC AGATGTACCA GTGTCAGAAA TCCTGAAGGC CATCCCCAAA 
TACAAGCTGG GAATCCATGG GTATGCCTTT GCCATCACCA ACAACGGATA CATCCTCACC 
CATCCGGACC TGCAGCCGCT GTATGAAGAA GGGAAAAAGA GGAGAAAGTC CAGTTACAGC 
AGCGCAGATC TGTCCGAGGT GGAGTGGGAG GACAAGGAGG ACGAGCTGCG TAACGCTATG 
GTGAACAGAC AGACGGGAAG CTTCTCTATG AAAGTCAAGA AAACTGTGGA TAAAGGGAAG 
CGGGTGCTTG TTCTTCACAA TGATTACTAC TACACAGACA TTAAAGGAAC GCCCTTCAGC 
TTAGGAGTGG CTCTGTCTGG AGGTCACGGC AAATATGTCT TCAGAGGAAA TATCTCTGTT 
GAAGAAGGGC TGCATGATTT GGAGCATCCA GACGTTGCAT TAGCAGATGA ATGGACATAC 
TGTAACACAG AGGAACATCC AGACCATCCC GTGCTCTCTC AGATTGAAGC CATTAAGCTG 
TATTTCAATG GAGAAGAGCA TCTGAAGTGT GATCAGGAGC TGGTGCAGGA GGTGTTGTTT 
GATGCGGTGG TCACGGCACC ACTGGAGGCG TACTGGACCA GCCTCGCTCT CAATAAATCC 
GAAAACTCTG ATAAAGGTGT GGAGATCTCT TTCCTCGCCA CTCGAACTGG ATTATCACGC 
ATCAACCTTT TTGTGACGCC AGATCAACTG AGCAATCAAG GCCTCCTGAC AGGAGAGGAT 
AAAGAGGTGC AGTTCAGCGC TGATCATTTC CCTCTGTGGT ACAGAAGAGC TGCTGAACAG 
GTGCCCGGCA CATTTGTCTA CAGCCTTCCG TATGACGCAG CCTCTGACAA TAAGAGTGTG 
GTCTTGGCCA GCAGCGCCAT CCATCTGCTG GATGAGAGAG AGTCACCCAT CGCTGCAGCT 
GTAGGTATTC AGATGAAGCT AGAGTTCTTT CAGAGGAAGT TCTGGACCGC CAGCAGACAG 
TGTGCGGCGC TTGATGGAAA GTGTTCAATC AGCTGTGATA ACGATGACAT CAGCTGCTAC 
CTCATAGATA ATAATGGATT TATTATTGTG GCCGAGGATC AGTCTTTGAC TGGTGTGTTT 
TTTGGAGAGC CTGAAGGAGC GGTGATGGGC AAACTGGTGT CAATGGGCTC ATTTAAGAGG 
GTGAATCTGT ATGACTACCG GGCCATCTGT AGGGTGTATG CGCAGACCAG CGACACAGCA 
CACACACTTC TACACCCCTA CTTTGCTCTG TTCGCAGCTA TCCGATGGCT CCTGACGGAG 
TTTGTCATAT TCCTGGTTGA GTTTAACCTG TACAGCTGGT GGCACTCTGA CCTCACCATT 
ACAGCTCACA GAGGAGGCAG AACTCTGCTG GTTCCCTGCG ACACCGAGTA TCCAGCCTTC 
GTGTCCGAGC GCACCATTAA AGAGACTGTG GGCAATATTG ACTGTGAAAG CTGTGTGAAA 
TCGTTTGTGA TTCAGCAGAT TCCCAGCAGT AATCTCTTCA TGGTGGTCAT TGATAACACA 
TGTGACTGCA GCGACTTCGG ACCCGTGACC ATGGAGCCTG TCGAAATCAT GTATAATGAG 
TCTCTGAAAT GTGAGCGGCT GAAGTTTCAG AAGGACAGAA GACGTCCAGA CACGTGCCAT 
CCATTCCATC ATGAGGAGAA CTCAATGGAG TGCGGTGGTT CAGTCAGTCT GTCTCCATCC 
TCTCCAGCTC TTCTAGTGCT AATAATCTCC ATCTTCAGCA GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA2D3A ( XM_009304342.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009302617.1 Danio rerio calcium channel, voltage-dependent, alpha 2/delta subunit 3a (cacna2d3a), transcript variant X1, mRNA.

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CloneID	ODa50126
Clone ID Related Accession (Same CDS sequence)	XM_009304342.3 , XM_009304342.2
Accession Version	XM_009304342.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3243bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent calcium channel subunit alpha-2/delta-3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009304342.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTGTG TTTTCTGTCG GCTGTTTGTC CTCCTCCTGT GGATTATTAA TGATTCAGGA 
ATATGTGCGT CTCATCAAGG GATCCCCCTG TCAGTTGTGA AACTTTGGGC TTCGGCGTTT 
GGCGGAGAGA TAAAATCCAT CGCAGCTAAA TATTCAGGCT CTCAGCTGCT GCAAAAGAAG 
TATAAGGAGT TTGAGCGGTC GGTGAGGGTG GAGGAGATCG ATGGCATCAA ACTGGTGAAG 
AAACTCGCTG CCAAAATGGA GCAGAGGTTT CACAAGAAAG CTGAAGCCAT AAAGCGTTTG 
GTGGAAGCAG CAGAAGAAGC ACACCTGAAC CACGAGGAGG ATCCTGACCT ACAGTACGAG 
TATTTTAATG CAGTGTTGAT CAATGAGTTG GACGAGGAGG GCCGCAGCGT GGAGCTGGGT 
GGAGAATTCA TCCTTCAGCC GAACGAACAT TTCAATAATC TGTCAGTAAA CCTCAGCCTC 
AGTGTCGTCC AGGTGCCAAC TAACATGTAC AACAAAGACT CAGACATCGT GAACGGGGTT 
TACTGGTCAG AGGCTCTCAA CACAGTGTTT GTGGACAACT TTAAAAGAGA CCCTTCTCTC 
CTATGGCAGT ATTTTGGCAG CGCTCAAGGT TTCTTCAGAC AGTATCCAGG AATGAAGTGG 
ACGCCTGATG AACACGGAGT TCTAGAGTTT GACTGTCGAA ATCGGAAATG GTATATCCAG 
GCCGCTACGT CTCCTAAAGA TGTGGTGATA TTGGTGGATG TGAGCGGCAG TATGAAGGGT 
TTACGGCTCA CCATCGCTAG ACAGACCGTC TCCTCTATAC TGGACACACT CGGAGACGAC 
GACTTCTTCA ACGTTATTGC TTACAACGAG GATCTGCATT ACGTGGAGCC GTGTCTGAAC 
GGAACATTAG TTCAGGCTGA CATTACCAAC AAAGATCATT TCCGCCAGCG TCTCGATAAG 
CTCCTCGCTA AAGGGATCGG CATGCTGGAC GTGGCTCTGA CAGAGGCCTT TGAGCTGCTC 
AGCAATTTTA ATCAGACAGG CAGAGGCAGT GAGTGCAGTC AGGCTATAAT GCTGGTGACA 
GACGGCGCAG TGGAGACATA TGATGCTGTG TTCGCCGTCT ACAACTGGCC TGACAGGAAG 
GTGCGTGTTT TCCCTTACCT GATTGGTCGA GAGTCTGCCT TCGCTGATAA CCTGAAGTGG 
ATGGCCTGCG CTAATAAAGG TTACTTCACC CAGATCTCCA CGCTGGCGGA CGTACAGGAG 
AACGTGATGA AGTATCTGCA TGTTCTCAGT CGGCCGAAAG TCATCGATCG AGAGCATGAC 
ATGGTGTGGA CTGAAGCCTA CATCGACAAC ACTCGTTCGC AGGCTCAGAA GCTGGAAGAC 
GCTCAGGGTC CAGTGCTCAT GACTACCGTG GCCATGCCTG TGTTCAGCAC CAAAAACGAG 
ACCAGGAATA AAGGGATTCT TCTTGGAGTG GTCGGCACAG ATGTACCAGT GTCAGAAATC 
CTGAAGGCCA TCCCCAAATA CAAGCTGGGA ATCCATGGGT ATGCCTTTGC CATCACCAAC 
AACGGATACA TCCTCACCCA TCCGGACCTG CAGCCGCTGT ATGAAGAAGG GAAAAAGAGG 
AGAAAGTCCA GTTACAGCAG CGCAGATCTG TCCGAGGTGG AGTGGGAGGA CAAGGAGGAC 
GAGCTGCGTA ACGCTATGGT GAACAGACAG ACGGGAAGCT TCTCTATGAA AGTCAAGAAA 
ACTGTGGATA AAGGGAAGCG GGTGCTTGTT CTTCACAATG ATTACTACTA CACAGACATT 
AAAGGAACGC CCTTCAGCTT AGGAGTGGCT CTGTCTGGAG GTCACGGCAA ATATGTCTTC 
AGAGGAAATA TCTCTGTTGA AGAAGGGCTG CATGATTTGG AGCATCCAGA CGTTGCATTA 
GCAGATGAAT GGACATACTG TAACACAGAG GAACATCCAG ACCATCCCGT GCTCTCTCAG 
ATTGAAGCCA TTAAGCTGTA TTTCAATGGA GAAGAGCATC TGAAGTGTGA TCAGGAGCTG 
GTGCAGGAGG TGTTGTTTGA TGCGGTGGTC ACGGCACCAC TGGAGGCGTA CTGGACCAGC 
CTCGCTCTCA ATAAATCCGA AAACTCTGAT AAAGGTGTGG AGATCTCTTT CCTCGCCACT 
CGAACTGGAT TATCACGCAT CAACCTTTTT GTGACGCCAG ATCAACTGAG CAATCAAGGC 
CTCCTGACAG GAGAGGATAA AGAGGTGCAG TTCAGCGCTG ATCATTTCCC TCTGTGGTAC 
AGAAGAGCTG CTGAACAGGT GCCCGGCACA TTTGTCTACA GCCTTCCGTA TGACGCAGCC 
TCTGACAATA AGAGTGTGGT CTTGGCCAGC AGCGCCATCC ATCTGCTGGA TGAGAGAGAG 
TCACCCATCG CTGCAGCTGT AGGTATTCAG ATGAAGCTAG AGTTCTTTCA GAGGAAGTTC 
TGGACCGCCA GCAGACAGTG TGCGGCGCTT GATGGAAAGT GTTCAATCAG CTGTGATAAC 
GATGACATCA GCTGCTACCT CATAGATAAT AATGGATTTA TTATTGTGGC CGAGGATCAG 
TCTTTGACTG GTGTGTTTTT TGGAGAGCCT GAAGGAGCGG TGATGGGCAA ACTGGTGTCA 
ATGGGCTCAT TTAAGAGGGT GAATCTGTAT GACTACCGGG CCATCTGTAG GGTGTATGCG 
CAGACCAGCG ACACAGCACA CACACTTCTA CACCCCTACT TTGCTCTGTT CGCAGCTATC 
CGATGGCTCC TGACGGAGTT TGTCATATTC CTGGTTGAGT TTAACCTGTA CAGCTGGTGG 
CACTCTGACC TCACCATTAC AGCTCACAGA GGAGGCAGAA CTCTGCTGGT TCCCTGCGAC 
ACCGAGTATC CAGCCTTCGT GTCCGAGCGC ACCATTAAAG AGACTGTGGG CAATATTGAC 
TGTGAAAGCT GTGTGAAATC GTTTGTGATT CAGCAGATTC CCAGCAGTAA TCTCTTCATG 
GTGGTCATTG ATAACACATG TGACTGCAGC GACTTCGGAC CCGTGACCAT GGAGCCTGTC 
GAAATCATGT ATAATGAGTC TCTGAAATGT GAGCGGCTGA AGTTTCAGAA GGACAGAAGA 
CGTCCAGACA CGTGCCATCC ATTCCATCAT GAGGAGAACT CAATGGAGTG CGGTGGTTCA 
GTCAGTCTGT CTCCATCCTC TCCAGCTCTT CTAGTGCTAA TAATCTCCAT CTTCAGCAGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009302617.1
CDS	184..3426
Translation	MKCVFCRLFVLLLWIINDSGICASHQGIPLSVVKLWASAFGGEIKSIAAKYSGSQLLQKKYKEFERSVRVEEIDGIKLVKKLAAKMEQRFHKKAEAIKRLVEAAEEAHLNHEEDPDLQYEYFNAVLINELDEEGRSVELGGEFILQPNEHFNNLSVNLSLSVVQVPTNMYNKDSDIVNGVYWSEALNTVFVDNFKRDPSLLWQYFGSAQGFFRQYPGMKWTPDEHGVLEFDCRNRKWYIQAATSPKDVVILVDVSGSMKGLRLTIARQTVSSILDTLGDDDFFNVIAYNEDLHYVEPCLNGTLVQADITNKDHFRQRLDKLLAKGIGMLDVALTEAFELLSNFNQTGRGSECSQAIMLVTDGAVETYDAVFAVYNWPDRKVRVFPYLIGRESAFADNLKWMACANKGYFTQISTLADVQENVMKYLHVLSRPKVIDREHDMVWTEAYIDNTRSQAQKLEDAQGPVLMTTVAMPVFSTKNETRNKGILLGVVGTDVPVSEILKAIPKYKLGIHGYAFAITNNGYILTHPDLQPLYEEGKKRRKSSYSSADLSEVEWEDKEDELRNAMVNRQTGSFSMKVKKTVDKGKRVLVLHNDYYYTDIKGTPFSLGVALSGGHGKYVFRGNISVEEGLHDLEHPDVALADEWTYCNTEEHPDHPVLSQIEAIKLYFNGEEHLKCDQELVQEVLFDAVVTAPLEAYWTSLALNKSENSDKGVEISFLATRTGLSRINLFVTPDQLSNQGLLTGEDKEVQFSADHFPLWYRRAAEQVPGTFVYSLPYDAASDNKSVVLASSAIHLLDERESPIAAAVGIQMKLEFFQRKFWTASRQCAALDGKCSISCDNDDISCYLIDNNGFIIVAEDQSLTGVFFGEPEGAVMGKLVSMGSFKRVNLYDYRAICRVYAQTSDTAHTLLHPYFALFAAIRWLLTEFVIFLVEFNLYSWWHSDLTITAHRGGRTLLVPCDTEYPAFVSERTIKETVGNIDCESCVKSFVIQQIPSSNLFMVVIDNTCDCSDFGPVTMEPVEIMYNESLKCERLKFQKDRRRPDTCHPFHHEENSMECGGSVSLSPSSPALLVLIISIFSR

Target ORF information:

RefSeq Version	XM_009304342.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, alpha 2/delta subunit 3a (cacna2d3a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009304342.3

ORF Insert Sequence:

ATGAAGTGTG TTTTCTGTCG GCTGTTTGTC CTCCTCCTGT GGATTATTAA TGATTCAGGA 
ATATGTGCGT CTCATCAAGG GATCCCCCTG TCAGTTGTGA AACTTTGGGC TTCGGCGTTT 
GGCGGAGAGA TAAAATCCAT CGCAGCTAAA TATTCAGGCT CTCAGCTGCT GCAAAAGAAG 
TATAAGGAGT TTGAGCGGTC GGTGAGGGTG GAGGAGATCG ATGGCATCAA ACTGGTGAAG 
AAACTCGCTG CCAAAATGGA GCAGAGGTTT CACAAGAAAG CTGAAGCCAT AAAGCGTTTG 
GTGGAAGCAG CAGAAGAAGC ACACCTGAAC CACGAGGAGG ATCCTGACCT ACAGTACGAG 
TATTTTAATG CAGTGTTGAT CAATGAGTTG GACGAGGAGG GCCGCAGCGT GGAGCTGGGT 
GGAGAATTCA TCCTTCAGCC GAACGAACAT TTCAATAATC TGTCAGTAAA CCTCAGCCTC 
AGTGTCGTCC AGGTGCCAAC TAACATGTAC AACAAAGACT CAGACATCGT GAACGGGGTT 
TACTGGTCAG AGGCTCTCAA CACAGTGTTT GTGGACAACT TTAAAAGAGA CCCTTCTCTC 
CTATGGCAGT ATTTTGGCAG CGCTCAAGGT TTCTTCAGAC AGTATCCAGG AATGAAGTGG 
ACGCCTGATG AACACGGAGT TCTAGAGTTT GACTGTCGAA ATCGGAAATG GTATATCCAG 
GCCGCTACGT CTCCTAAAGA TGTGGTGATA TTGGTGGATG TGAGCGGCAG TATGAAGGGT 
TTACGGCTCA CCATCGCTAG ACAGACCGTC TCCTCTATAC TGGACACACT CGGAGACGAC 
GACTTCTTCA ACGTTATTGC TTACAACGAG GATCTGCATT ACGTGGAGCC GTGTCTGAAC 
GGAACATTAG TTCAGGCTGA CATTACCAAC AAAGATCATT TCCGCCAGCG TCTCGATAAG 
CTCCTCGCTA AAGGGATCGG CATGCTGGAC GTGGCTCTGA CAGAGGCCTT TGAGCTGCTC 
AGCAATTTTA ATCAGACAGG CAGAGGCAGT GAGTGCAGTC AGGCTATAAT GCTGGTGACA 
GACGGCGCAG TGGAGACATA TGATGCTGTG TTCGCCGTCT ACAACTGGCC TGACAGGAAG 
GTGCGTGTTT TCCCTTACCT GATTGGTCGA GAGTCTGCCT TCGCTGATAA CCTGAAGTGG 
ATGGCCTGCG CTAATAAAGG TTACTTCACC CAGATCTCCA CGCTGGCGGA CGTACAGGAG 
AACGTGATGA AGTATCTGCA TGTTCTCAGT CGGCCGAAAG TCATCGATCG AGAGCATGAC 
ATGGTGTGGA CTGAAGCCTA CATCGACAAC ACTCGTTCGC AGGCTCAGAA GCTGGAAGAC 
GCTCAGGGTC CAGTGCTCAT GACTACCGTG GCCATGCCTG TGTTCAGCAC CAAAAACGAG 
ACCAGGAATA AAGGGATTCT TCTTGGAGTG GTCGGCACAG ATGTACCAGT GTCAGAAATC 
CTGAAGGCCA TCCCCAAATA CAAGCTGGGA ATCCATGGGT ATGCCTTTGC CATCACCAAC 
AACGGATACA TCCTCACCCA TCCGGACCTG CAGCCGCTGT ATGAAGAAGG GAAAAAGAGG 
AGAAAGTCCA GTTACAGCAG CGCAGATCTG TCCGAGGTGG AGTGGGAGGA CAAGGAGGAC 
GAGCTGCGTA ACGCTATGGT GAACAGACAG ACGGGAAGCT TCTCTATGAA AGTCAAGAAA 
ACTGTGGATA AAGGGAAGCG GGTGCTTGTT CTTCACAATG ATTACTACTA CACAGACATT 
AAAGGAACGC CCTTCAGCTT AGGAGTGGCT CTGTCTGGAG GTCACGGCAA ATATGTCTTC 
AGAGGAAATA TCTCTGTTGA AGAAGGGCTG CATGATTTGG AGCATCCAGA CGTTGCATTA 
GCAGATGAAT GGACATACTG TAACACAGAG GAACATCCAG ACCATCCCGT GCTCTCTCAG 
ATTGAAGCCA TTAAGCTGTA TTTCAATGGA GAAGAGCATC TGAAGTGTGA TCAGGAGCTG 
GTGCAGGAGG TGTTGTTTGA TGCGGTGGTC ACGGCACCAC TGGAGGCGTA CTGGACCAGC 
CTCGCTCTCA ATAAATCCGA AAACTCTGAT AAAGGTGTGG AGATCTCTTT CCTCGCCACT 
CGAACTGGAT TATCACGCAT CAACCTTTTT GTGACGCCAG ATCAACTGAG CAATCAAGGC 
CTCCTGACAG GAGAGGATAA AGAGGTGCAG TTCAGCGCTG ATCATTTCCC TCTGTGGTAC 
AGAAGAGCTG CTGAACAGGT GCCCGGCACA TTTGTCTACA GCCTTCCGTA TGACGCAGCC 
TCTGACAATA AGAGTGTGGT CTTGGCCAGC AGCGCCATCC ATCTGCTGGA TGAGAGAGAG 
TCACCCATCG CTGCAGCTGT AGGTATTCAG ATGAAGCTAG AGTTCTTTCA GAGGAAGTTC 
TGGACCGCCA GCAGACAGTG TGCGGCGCTT GATGGAAAGT GTTCAATCAG CTGTGATAAC 
GATGACATCA GCTGCTACCT CATAGATAAT AATGGATTTA TTATTGTGGC CGAGGATCAG 
TCTTTGACTG GTGTGTTTTT TGGAGAGCCT GAAGGAGCGG TGATGGGCAA ACTGGTGTCA 
ATGGGCTCAT TTAAGAGGGT GAATCTGTAT GACTACCGGG CCATCTGTAG GGTGTATGCG 
CAGACCAGCG ACACAGCACA CACACTTCTA CACCCCTACT TTGCTCTGTT CGCAGCTATC 
CGATGGCTCC TGACGGAGTT TGTCATATTC CTGGTTGAGT TTAACCTGTA CAGCTGGTGG 
CACTCTGACC TCACCATTAC AGCTCACAGA GGAGGCAGAA CTCTGCTGGT TCCCTGCGAC 
ACCGAGTATC CAGCCTTCGT GTCCGAGCGC ACCATTAAAG AGACTGTGGG CAATATTGAC 
TGTGAAAGCT GTGTGAAATC GTTTGTGATT CAGCAGATTC CCAGCAGTAA TCTCTTCATG 
GTGGTCATTG ATAACACATG TGACTGCAGC GACTTCGGAC CCGTGACCAT GGAGCCTGTC 
GAAATCATGT ATAATGAGTC TCTGAAATGT GAGCGGCTGA AGTTTCAGAA GGACAGAAGA 
CGTCCAGACA CGTGCCATCC ATTCCATCAT GAGGAGAACT CAATGGAGTG CGGTGGTTCA 
GTCAGTCTGT CTCCATCCTC TCCAGCTCTT CTAGTGCTAA TAATCTCCAT CTTCAGCAGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA2D3A ( XM_009304342.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009302617.1 Danio rerio calcium channel, voltage-dependent, alpha 2/delta subunit 3a (cacna2d3a), mRNA.

Price & Availability↑

CloneID	ODa50126
Clone ID Related Accession (Same CDS sequence)	XM_009304342.3 , XM_009304342.2
Accession Version	XM_009304342.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3243bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	voltage-dependent calcium channel subunit alpha-2/delta-3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009304342.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTGTG TTTTCTGTCG GCTGTTTGTC CTCCTCCTGT GGATTATTAA TGATTCAGGA 
ATATGTGCGT CTCATCAAGG GATCCCCCTG TCAGTTGTGA AACTTTGGGC TTCGGCGTTT 
GGCGGAGAGA TAAAATCCAT CGCAGCTAAA TATTCAGGCT CTCAGCTGCT GCAAAAGAAG 
TATAAGGAGT TTGAGCGGTC GGTGAGGGTG GAGGAGATCG ATGGCATCAA ACTGGTGAAG 
AAACTCGCTG CCAAAATGGA GCAGAGGTTT CACAAGAAAG CTGAAGCCAT AAAGCGTTTG 
GTGGAAGCAG CAGAAGAAGC ACACCTGAAC CACGAGGAGG ATCCTGACCT ACAGTACGAG 
TATTTTAATG CAGTGTTGAT CAATGAGTTG GACGAGGAGG GCCGCAGCGT GGAGCTGGGT 
GGAGAATTCA TCCTTCAGCC GAACGAACAT TTCAATAATC TGTCAGTAAA CCTCAGCCTC 
AGTGTCGTCC AGGTGCCAAC TAACATGTAC AACAAAGACT CAGACATCGT GAACGGGGTT 
TACTGGTCAG AGGCTCTCAA CACAGTGTTT GTGGACAACT TTAAAAGAGA CCCTTCTCTC 
CTATGGCAGT ATTTTGGCAG CGCTCAAGGT TTCTTCAGAC AGTATCCAGG AATGAAGTGG 
ACGCCTGATG AACACGGAGT TCTAGAGTTT GACTGTCGAA ATCGGAAATG GTATATCCAG 
GCCGCTACGT CTCCTAAAGA TGTGGTGATA TTGGTGGATG TGAGCGGCAG TATGAAGGGT 
TTACGGCTCA CCATCGCTAG ACAGACCGTC TCCTCTATAC TGGACACACT CGGAGACGAC 
GACTTCTTCA ACGTTATTGC TTACAACGAG GATCTGCATT ACGTGGAGCC GTGTCTGAAC 
GGAACATTAG TTCAGGCTGA CATTACCAAC AAAGATCATT TCCGCCAGCG TCTCGATAAG 
CTCCTCGCTA AAGGGATCGG CATGCTGGAC GTGGCTCTGA CAGAGGCCTT TGAGCTGCTC 
AGCAATTTTA ATCAGACAGG CAGAGGCAGT GAGTGCAGTC AGGCTATAAT GCTGGTGACA 
GACGGCGCAG TGGAGACATA TGATGCTGTG TTCGCCGTCT ACAACTGGCC TGACAGGAAG 
GTGCGTGTTT TCCCTTACCT GATTGGTCGA GAGTCTGCCT TCGCTGATAA CCTGAAGTGG 
ATGGCCTGCG CTAATAAAGG TTACTTCACC CAGATCTCCA CGCTGGCGGA CGTACAGGAG 
AACGTGATGA AGTATCTGCA TGTTCTCAGT CGGCCGAAAG TCATCGATCG AGAGCATGAC 
ATGGTGTGGA CTGAAGCCTA CATCGACAAC ACTCGTTCGC AGGCTCAGAA GCTGGAAGAC 
GCTCAGGGTC CAGTGCTCAT GACTACCGTG GCCATGCCTG TGTTCAGCAC CAAAAACGAG 
ACCAGGAATA AAGGGATTCT TCTTGGAGTG GTCGGCACAG ATGTACCAGT GTCAGAAATC 
CTGAAGGCCA TCCCCAAATA CAAGCTGGGA ATCCATGGGT ATGCCTTTGC CATCACCAAC 
AACGGATACA TCCTCACCCA TCCGGACCTG CAGCCGCTGT ATGAAGAAGG GAAAAAGAGG 
AGAAAGTCCA GTTACAGCAG CGCAGATCTG TCCGAGGTGG AGTGGGAGGA CAAGGAGGAC 
GAGCTGCGTA ACGCTATGGT GAACAGACAG ACGGGAAGCT TCTCTATGAA AGTCAAGAAA 
ACTGTGGATA AAGGGAAGCG GGTGCTTGTT CTTCACAATG ATTACTACTA CACAGACATT 
AAAGGAACGC CCTTCAGCTT AGGAGTGGCT CTGTCTGGAG GTCACGGCAA ATATGTCTTC 
AGAGGAAATA TCTCTGTTGA AGAAGGGCTG CATGATTTGG AGCATCCAGA CGTTGCATTA 
GCAGATGAAT GGACATACTG TAACACAGAG GAACATCCAG ACCATCCCGT GCTCTCTCAG 
ATTGAAGCCA TTAAGCTGTA TTTCAATGGA GAAGAGCATC TGAAGTGTGA TCAGGAGCTG 
GTGCAGGAGG TGTTGTTTGA TGCGGTGGTC ACGGCACCAC TGGAGGCGTA CTGGACCAGC 
CTCGCTCTCA ATAAATCCGA AAACTCTGAT AAAGGTGTGG AGATCTCTTT CCTCGCCACT 
CGAACTGGAT TATCACGCAT CAACCTTTTT GTGACGCCAG ATCAACTGAG CAATCAAGGC 
CTCCTGACAG GAGAGGATAA AGAGGTGCAG TTCAGCGCTG ATCATTTCCC TCTGTGGTAC 
AGAAGAGCTG CTGAACAGGT GCCCGGCACA TTTGTCTACA GCCTTCCGTA TGACGCAGCC 
TCTGACAATA AGAGTGTGGT CTTGGCCAGC AGCGCCATCC ATCTGCTGGA TGAGAGAGAG 
TCACCCATCG CTGCAGCTGT AGGTATTCAG ATGAAGCTAG AGTTCTTTCA GAGGAAGTTC 
TGGACCGCCA GCAGACAGTG TGCGGCGCTT GATGGAAAGT GTTCAATCAG CTGTGATAAC 
GATGACATCA GCTGCTACCT CATAGATAAT AATGGATTTA TTATTGTGGC CGAGGATCAG 
TCTTTGACTG GTGTGTTTTT TGGAGAGCCT GAAGGAGCGG TGATGGGCAA ACTGGTGTCA 
ATGGGCTCAT TTAAGAGGGT GAATCTGTAT GACTACCGGG CCATCTGTAG GGTGTATGCG 
CAGACCAGCG ACACAGCACA CACACTTCTA CACCCCTACT TTGCTCTGTT CGCAGCTATC 
CGATGGCTCC TGACGGAGTT TGTCATATTC CTGGTTGAGT TTAACCTGTA CAGCTGGTGG 
CACTCTGACC TCACCATTAC AGCTCACAGA GGAGGCAGAA CTCTGCTGGT TCCCTGCGAC 
ACCGAGTATC CAGCCTTCGT GTCCGAGCGC ACCATTAAAG AGACTGTGGG CAATATTGAC 
TGTGAAAGCT GTGTGAAATC GTTTGTGATT CAGCAGATTC CCAGCAGTAA TCTCTTCATG 
GTGGTCATTG ATAACACATG TGACTGCAGC GACTTCGGAC CCGTGACCAT GGAGCCTGTC 
GAAATCATGT ATAATGAGTC TCTGAAATGT GAGCGGCTGA AGTTTCAGAA GGACAGAAGA 
CGTCCAGACA CGTGCCATCC ATTCCATCAT GAGGAGAACT CAATGGAGTG CGGTGGTTCA 
GTCAGTCTGT CTCCATCCTC TCCAGCTCTT CTAGTGCTAA TAATCTCCAT CTTCAGCAGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009302617.1
CDS	186..3428
Translation	MKCVFCRLFVLLLWIINDSGICASHQGIPLSVVKLWASAFGGEIKSIAAKYSGSQLLQKKYKEFERSVRVEEIDGIKLVKKLAAKMEQRFHKKAEAIKRLVEAAEEAHLNHEEDPDLQYEYFNAVLINELDEEGRSVELGGEFILQPNEHFNNLSVNLSLSVVQVPTNMYNKDSDIVNGVYWSEALNTVFVDNFKRDPSLLWQYFGSAQGFFRQYPGMKWTPDEHGVLEFDCRNRKWYIQAATSPKDVVILVDVSGSMKGLRLTIARQTVSSILDTLGDDDFFNVIAYNEDLHYVEPCLNGTLVQADITNKDHFRQRLDKLLAKGIGMLDVALTEAFELLSNFNQTGRGSECSQAIMLVTDGAVETYDAVFAVYNWPDRKVRVFPYLIGRESAFADNLKWMACANKGYFTQISTLADVQENVMKYLHVLSRPKVIDREHDMVWTEAYIDNTRSQAQKLEDAQGPVLMTTVAMPVFSTKNETRNKGILLGVVGTDVPVSEILKAIPKYKLGIHGYAFAITNNGYILTHPDLQPLYEEGKKRRKSSYSSADLSEVEWEDKEDELRNAMVNRQTGSFSMKVKKTVDKGKRVLVLHNDYYYTDIKGTPFSLGVALSGGHGKYVFRGNISVEEGLHDLEHPDVALADEWTYCNTEEHPDHPVLSQIEAIKLYFNGEEHLKCDQELVQEVLFDAVVTAPLEAYWTSLALNKSENSDKGVEISFLATRTGLSRINLFVTPDQLSNQGLLTGEDKEVQFSADHFPLWYRRAAEQVPGTFVYSLPYDAASDNKSVVLASSAIHLLDERESPIAAAVGIQMKLEFFQRKFWTASRQCAALDGKCSISCDNDDISCYLIDNNGFIIVAEDQSLTGVFFGEPEGAVMGKLVSMGSFKRVNLYDYRAICRVYAQTSDTAHTLLHPYFALFAAIRWLLTEFVIFLVEFNLYSWWHSDLTITAHRGGRTLLVPCDTEYPAFVSERTIKETVGNIDCESCVKSFVIQQIPSSNLFMVVIDNTCDCSDFGPVTMEPVEIMYNESLKCERLKFQKDRRRPDTCHPFHHEENSMECGGSVSLSPSSPALLVLIISIFSR

Target ORF information:

RefSeq Version	XM_009304342.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, alpha 2/delta subunit 3a (cacna2d3a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009304342.2

ORF Insert Sequence:

ATGAAGTGTG TTTTCTGTCG GCTGTTTGTC CTCCTCCTGT GGATTATTAA TGATTCAGGA 
ATATGTGCGT CTCATCAAGG GATCCCCCTG TCAGTTGTGA AACTTTGGGC TTCGGCGTTT 
GGCGGAGAGA TAAAATCCAT CGCAGCTAAA TATTCAGGCT CTCAGCTGCT GCAAAAGAAG 
TATAAGGAGT TTGAGCGGTC GGTGAGGGTG GAGGAGATCG ATGGCATCAA ACTGGTGAAG 
AAACTCGCTG CCAAAATGGA GCAGAGGTTT CACAAGAAAG CTGAAGCCAT AAAGCGTTTG 
GTGGAAGCAG CAGAAGAAGC ACACCTGAAC CACGAGGAGG ATCCTGACCT ACAGTACGAG 
TATTTTAATG CAGTGTTGAT CAATGAGTTG GACGAGGAGG GCCGCAGCGT GGAGCTGGGT 
GGAGAATTCA TCCTTCAGCC GAACGAACAT TTCAATAATC TGTCAGTAAA CCTCAGCCTC 
AGTGTCGTCC AGGTGCCAAC TAACATGTAC AACAAAGACT CAGACATCGT GAACGGGGTT 
TACTGGTCAG AGGCTCTCAA CACAGTGTTT GTGGACAACT TTAAAAGAGA CCCTTCTCTC 
CTATGGCAGT ATTTTGGCAG CGCTCAAGGT TTCTTCAGAC AGTATCCAGG AATGAAGTGG 
ACGCCTGATG AACACGGAGT TCTAGAGTTT GACTGTCGAA ATCGGAAATG GTATATCCAG 
GCCGCTACGT CTCCTAAAGA TGTGGTGATA TTGGTGGATG TGAGCGGCAG TATGAAGGGT 
TTACGGCTCA CCATCGCTAG ACAGACCGTC TCCTCTATAC TGGACACACT CGGAGACGAC 
GACTTCTTCA ACGTTATTGC TTACAACGAG GATCTGCATT ACGTGGAGCC GTGTCTGAAC 
GGAACATTAG TTCAGGCTGA CATTACCAAC AAAGATCATT TCCGCCAGCG TCTCGATAAG 
CTCCTCGCTA AAGGGATCGG CATGCTGGAC GTGGCTCTGA CAGAGGCCTT TGAGCTGCTC 
AGCAATTTTA ATCAGACAGG CAGAGGCAGT GAGTGCAGTC AGGCTATAAT GCTGGTGACA 
GACGGCGCAG TGGAGACATA TGATGCTGTG TTCGCCGTCT ACAACTGGCC TGACAGGAAG 
GTGCGTGTTT TCCCTTACCT GATTGGTCGA GAGTCTGCCT TCGCTGATAA CCTGAAGTGG 
ATGGCCTGCG CTAATAAAGG TTACTTCACC CAGATCTCCA CGCTGGCGGA CGTACAGGAG 
AACGTGATGA AGTATCTGCA TGTTCTCAGT CGGCCGAAAG TCATCGATCG AGAGCATGAC 
ATGGTGTGGA CTGAAGCCTA CATCGACAAC ACTCGTTCGC AGGCTCAGAA GCTGGAAGAC 
GCTCAGGGTC CAGTGCTCAT GACTACCGTG GCCATGCCTG TGTTCAGCAC CAAAAACGAG 
ACCAGGAATA AAGGGATTCT TCTTGGAGTG GTCGGCACAG ATGTACCAGT GTCAGAAATC 
CTGAAGGCCA TCCCCAAATA CAAGCTGGGA ATCCATGGGT ATGCCTTTGC CATCACCAAC 
AACGGATACA TCCTCACCCA TCCGGACCTG CAGCCGCTGT ATGAAGAAGG GAAAAAGAGG 
AGAAAGTCCA GTTACAGCAG CGCAGATCTG TCCGAGGTGG AGTGGGAGGA CAAGGAGGAC 
GAGCTGCGTA ACGCTATGGT GAACAGACAG ACGGGAAGCT TCTCTATGAA AGTCAAGAAA 
ACTGTGGATA AAGGGAAGCG GGTGCTTGTT CTTCACAATG ATTACTACTA CACAGACATT 
AAAGGAACGC CCTTCAGCTT AGGAGTGGCT CTGTCTGGAG GTCACGGCAA ATATGTCTTC 
AGAGGAAATA TCTCTGTTGA AGAAGGGCTG CATGATTTGG AGCATCCAGA CGTTGCATTA 
GCAGATGAAT GGACATACTG TAACACAGAG GAACATCCAG ACCATCCCGT GCTCTCTCAG 
ATTGAAGCCA TTAAGCTGTA TTTCAATGGA GAAGAGCATC TGAAGTGTGA TCAGGAGCTG 
GTGCAGGAGG TGTTGTTTGA TGCGGTGGTC ACGGCACCAC TGGAGGCGTA CTGGACCAGC 
CTCGCTCTCA ATAAATCCGA AAACTCTGAT AAAGGTGTGG AGATCTCTTT CCTCGCCACT 
CGAACTGGAT TATCACGCAT CAACCTTTTT GTGACGCCAG ATCAACTGAG CAATCAAGGC 
CTCCTGACAG GAGAGGATAA AGAGGTGCAG TTCAGCGCTG ATCATTTCCC TCTGTGGTAC 
AGAAGAGCTG CTGAACAGGT GCCCGGCACA TTTGTCTACA GCCTTCCGTA TGACGCAGCC 
TCTGACAATA AGAGTGTGGT CTTGGCCAGC AGCGCCATCC ATCTGCTGGA TGAGAGAGAG 
TCACCCATCG CTGCAGCTGT AGGTATTCAG ATGAAGCTAG AGTTCTTTCA GAGGAAGTTC 
TGGACCGCCA GCAGACAGTG TGCGGCGCTT GATGGAAAGT GTTCAATCAG CTGTGATAAC 
GATGACATCA GCTGCTACCT CATAGATAAT AATGGATTTA TTATTGTGGC CGAGGATCAG 
TCTTTGACTG GTGTGTTTTT TGGAGAGCCT GAAGGAGCGG TGATGGGCAA ACTGGTGTCA 
ATGGGCTCAT TTAAGAGGGT GAATCTGTAT GACTACCGGG CCATCTGTAG GGTGTATGCG 
CAGACCAGCG ACACAGCACA CACACTTCTA CACCCCTACT TTGCTCTGTT CGCAGCTATC 
CGATGGCTCC TGACGGAGTT TGTCATATTC CTGGTTGAGT TTAACCTGTA CAGCTGGTGG 
CACTCTGACC TCACCATTAC AGCTCACAGA GGAGGCAGAA CTCTGCTGGT TCCCTGCGAC 
ACCGAGTATC CAGCCTTCGT GTCCGAGCGC ACCATTAAAG AGACTGTGGG CAATATTGAC 
TGTGAAAGCT GTGTGAAATC GTTTGTGATT CAGCAGATTC CCAGCAGTAA TCTCTTCATG 
GTGGTCATTG ATAACACATG TGACTGCAGC GACTTCGGAC CCGTGACCAT GGAGCCTGTC 
GAAATCATGT ATAATGAGTC TCTGAAATGT GAGCGGCTGA AGTTTCAGAA GGACAGAAGA 
CGTCCAGACA CGTGCCATCC ATTCCATCAT GAGGAGAACT CAATGGAGTG CGGTGGTTCA 
GTCAGTCTGT CTCCATCCTC TCCAGCTCTT CTAGTGCTAA TAATCTCCAT CTTCAGCAGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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CACNA2D3A cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Eメール

電話

FAX

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