stj cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	stj
Entrez Gene ID	36526
Full Name	straightjacket
Synonyms	CG12295,Dmel\CG12295,alpha[[2]]delta,alpha[[2]]delta-3,dalpha[[2]]delta-3,l(2)k10814
General protein information	Preferred Names straightjacket Names CG12295 gene product from transcript CG12295-RB CG12295-PB CG12295-PC CG12295-PD lethal (2) k10814 stj-PB stj-PC stj-PD
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 50C3-50C6

mRNA and Protein(s)

mRNA	Protein	Name
NM_137058.4	NP_610902.1	straightjacket, isoform B
NM_001259374.2	NP_001246303.1	straightjacket, isoform C
NM_001259375.2	NP_001246304.1	straightjacket, isoform D

Pathways from BioSystems

Homologies

Homo sapiens (human)	CACNA2D3	NP_060868.2
Pan troglodytes (chimpanzee)	CACNA2D3	XP_516539.3
Canis lupus familiaris (dog)	CACNA2D3	XP_005633241.1
Mus musculus (house mouse)	Cacna2d3	NP_033915.1
Rattus norvegicus (Norway rat)	Cacna2d3	NP_783185.1
Bos taurus (cattle)	CACNA2D3	NP_001193680.1
Danio rerio (zebrafish)	cacna2d3	XP_005172562.1
Drosophila melanogaster (fruit fly)	stj	NP_001246303.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005490	XP_315491.4
Gallus gallus (chicken)	CACNA2D3	XP_414338.2
Xenopus tropicalis (tropical clawed frog)	cacna2d3	XP_004914404.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

stj is required for noxious heat avoidance in adult Drosophila
Title: A genome-wide Drosophila screen for heat nociception identifies ?2?3 as an evolutionarily conserved pain gene.

In a screen for genes affecting synaptic function, straightjacket (stj), which encodes a Drosophila melanogaster alpha2delta similar to vertebrate alpha2delta3; stj mutants exhibit a severe reduction in Ca2+-dependent evoked neurotransmitter release.
Title: straightjacket is required for the synaptic stabilization of cacophony, a voltage-gated calcium channel alpha1 subunit.

The following stj gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the stj cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa09908	NM_137058.4 Latest version!	Drosophila melanogaster straightjacket (stj), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa13014	NM_001259375.2 Latest version!	Drosophila melanogaster straightjacket (stj), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa13015	NM_001259374.2 Latest version!	Drosophila melanogaster straightjacket (stj), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

stj ( NM_137058.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_610902.1 Drosophila melanogaster straightjacket (stj), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa09908
Clone ID Related Accession (Same CDS sequence)	NM_137058.4
Accession Version	NM_137058.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3657bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	straightjacket, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_137058.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCTGGT CCCGTCTCCT GGCCTGGTGG CGCCTCTGCT TGGGCCTGCT CTCCGTTCTG 
GTCTGTTTGG CACCGTGCGT CAATCTGCAG CCAGCCGAGA ACATCAACTA CAATCTCGTT 
CATTCGTGGG CCGACAAATT GGGCATGGAG CTGTTTCATC TGGGCGATTT CATCACCAGG 
CGCAAGGAGG TGCAAGAGAG TTTCAAGGAT GCAAAGGTCG TTTCGCGCAA CGGCGCTTCT 
ATTGTCGATT CGATGGCCAA GGAAATCGAA ATGATGATGG ACCTAAAAGT CAGTGCAGTG 
AGGCGAATCA TGGACACGGC TGAGAACACG GCGTTGTCGC ATCAGAACGA CATGGCGGAC 
AAGATGTTCT CCTATTATAG TGCCAAGGAG ATGCTGGAGC CCGGCGATCC AGTCCCGCCC 
ATTCCCACAC CGGCTCCCGA CATGGACAAG GATATAGGCG AGCCGCTGAT CTACGTCCAG 
CCGAAGGTGG TGGTGTTGGA ACCGCGTCCG GAATTCCACA ATACTCCGGT CAACTTTAGC 
GTCAGTTCGG TCCACGTGCC AGTCAATGTG TTCGATCGAG CACCGGATGT GATAAAAGCA 
ATTCAGTGGT CGGAGAATTT GGATCAGATA TTCCGTGACA ATTACAAAAA CGATCCGACA 
TTGTCGTGGC AATTCTTTGG CAGTTCGACT GGCTTTATGC GTCAATTCCC TGCATCGAAG 
TGGCGAAAGG ACGTACCGGT CGATCTATAC GATTGCCGAC TACGCTCCTG GTACATGGAG 
GCGGCGACGA GTCCGAAGGA TATTGTTATC CTGATGGATG GATCCGGTTC AATGTTGGGA 
CAGAGGCTAG ATATCGCAAA GCATGTTGTC AATACGATAC TCGATACATT AGGTACAAAT 
GACTTTGTGA ACATCTTCAC CTTTGATAAG GAAGTGAGCC CAGTCGTCCC ATGTTTCGAA 
GACACACTGA TTCAAGCCAA TCTGGGCAAC ATACGCGAAC TGAAAGAAGG GATTGAACTG 
TTTAGACCCA AATCGATCGC CAATTATACC GCTGCATTGA CAAAGGCATT CGAGCTATTG 
GAAGAGACCA AGTTGAGTTC GCGCGGGGCG CAGTGCAATC AGGCGATCAT GATCATTGGC 
GACGGGGCGC CGGAGAACAA TCGCGAGGTC TTCGAGTTGC ACAATTGGCG GGACCCGCCG 
TACAAGCCGG TCCGTGTGTT CACCTACCTG ATTGGCAAGG AGGTGGCCAA TTGGGATGAT 
ATACGGTGGA TGGCGTGCGA GAATCAGGGC TACTACGTCC ACCTCAGCGA CACCGCCGAG 
GTGCGCGAGA TGGTCTTGAA TTACATACCG GTTATGGCCA GGCCGCTCGT CCTGGGCAGG 
CACGATCATC CGGTGATTTG GTCGCAGGTC TATGCGGACA TCGAGGACAC GAAGCTATCC 
GACTATCTGT GGGACATTAA TCAGTGCGAG TACCAGAAGG CCGATGTCCT CGAGTATTGG 
CAGGTGCACG ATCGGATGCT GGAGCCCAGC GAAATGCACC GCCGGAAGTA CAGAAGGATG 
AAGGAGACCT GGAACCAGCC GGTGGACTCG AATGTGTACC AATTTATGAC CACCGTATCG 
ATGCCCATCT ACGATCGTCG CGAGAACGCG ACTAGGATTG CGAATATATT GGGCGTGGCC 
GGCACGGATG TGCCCATCAA TGAAATCAAA AAGCTGCTGT CGCCGTTCAC GCTCGGCGTG 
AATGGCTATG CGTTTATAGT AACCAACAAT GGTTATGTAC TATTCCATCC AGACTTTCGT 
CCAATTTTCC AAGGCTACAT ATTAAAGCCA GCATACAATA GCGTTGACAT GATTGAAGTC 
GAGCTATTGG ATGACGATCG ACCCGCCAGA GACTTTAATC CAGTGCTGAT GACGATAAGA 
GATTCTATAA TCAATCAATC GACAGGCAGC AAATGGATGT TAGTCAAAAA TCATTTCGAT 
GAAATGAAAC GGGTGGCACG CGTAAAGCGA CAATACTACT GGACGGCCAT CAAGAAGACG 
CCCTTTACTC TGGTCATTTC CTATCCGGAG CAATACGGCG TGAGTCACAT GGACATCCGA 
GCTGACCAGG AGATCCATCG CATAAGCATA AAGGGCACAA ACTTGCGCAG CGTTTTCAGC 
GGCAAGCGGT GGAAAATTCA TCCCGACTGG CTATTTTGTA AGCACAGCAA CAGGACATTC 
AAAACGCCAG AGATCGAACT GCTGTACTTT CTCGAGCGTA TGTCTGAGCC TGGCTGGCGA 
TGGCCGGGTA GTCGGAGTGC TATGCCCCCG GAGCACGCGG CCGCCATGTT TTGCGATCGG 
CAGCTAATGC AGGCTCTGGT CTTCGATGCC CGAGTCACCG GGTGGTTCTC CAACAATACC 
AGCTTTAACT CCAAGGACGA CAAAGGAAAC GAATTCAAGC AGCGTTTTGG CGTCACCGTC 
GCCTTTTTGG CCACCCACAG TGGTCTCACC CGCTGGCATG AGTTCCACTC GAATGCTGCC 
GAGGAATCCG GAGTCGGCGA GACTTTTAGC CAAAATAACA CGCGGGCCAT CGATGAGATT 
TGGTACAAAC GCGCCGTGGA CCAACATTTT GTGCGGGAGG AGAGCTTCGT TTACTCCGTG 
CCCTTCGATG CGGGTGAAAG CAACTCAGAG ATCCTGGTGA CCGCCAGTCA TGCCGTTTTC 
CACAATGAGG GCGGCAAGAC TGCACCGGCG GCTGTTGTGG GCTTCCAGTT TCAGCATTCG 
GCGCTCTACA AGCTCTTCCA CAACATCACC GGCAATGCCT GTGCCGTGGA TGACAAAGAC 
TGCTATATAC TGGACAACAA TGGCTATGTG ATCATTTCGA CGAGGGTCCA CGAGACGGGT 
CGATTCTTTG GCGAGGTGAA CGGAGCGATC ATGAAGCGAC TACTGGAGGA GAATGTCTAC 
AGGCAGGTCA CTGTCTACGA TTACCAGGCG GTGTGCTTCG AGTCCAAGAA CGATAACAAC 
GCCTCCAGCA TGCTGCTTTC GCCCCTGTTC CATCTGCTGC GCGTGGGCAA ATGGCTGCTG 
CATACGGCGC TGTGGTATAT TGTACAACTG TTGCAGTGGG CGCCCGGTGT GTCCAGCCAC 
TATGCGGATA TGTATGGTGA CTCCAACGAC ACGGAGCCAC CGCCGCCGGA GCCGCATCCG 
GATCACCATG CTCGCAACGG CAATGGGCAT GGGAAGAAGG ACGATGACCA CTGGCTGCGC 
TACTTGACAC TCCATCGCAC CCGGCTCAAG CCGTGCGACA TGAAGCGCGA TCTGTACACG 
CTGTTCAATG AAAAGGACAA CGTTGTCTAC AATATGACAG CCCATGCATG TGAGCGGCCA 
TTTGTCGTCC TGCCCATCCC GTTCAGCAAC CTCATCCTGC TGGTCATCGA CCAGCTGTGT 
CCCAGGGACG GCTCAGTCGT CCTCACGGTG AATCCGCAGC CGATCGACTA TCATCTGTCG 
GTCAACGATT CGTTGGCATG CTACAAGCAG GCTCGCGAGT TCAACCGCAT GCGACCACAC 
AGCTGCATCA GTCGGCACGC GAACGAGAGC GGCATTAAGC TGTGCGGAAA GGCCTGCTCC 
GTGTACGCCA ACCTGGGGCT GCTGCTCCTC TGTCACATAC TGAGTCGTTG GCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_610902.1
CDS	810..4466
Translation	MAWSRLLAWWRLCLGLLSVLVCLAPCVNLQPAENINYNLVHSWADKLGMELFHLGDFITRRKEVQESFKDAKVVSRNGASIVDSMAKEIEMMMDLKVSAVRRIMDTAENTALSHQNDMADKMFSYYSAKEMLEPGDPVPPIPTPAPDMDKDIGEPLIYVQPKVVVLEPRPEFHNTPVNFSVSSVHVPVNVFDRAPDVIKAIQWSENLDQIFRDNYKNDPTLSWQFFGSSTGFMRQFPASKWRKDVPVDLYDCRLRSWYMEAATSPKDIVILMDGSGSMLGQRLDIAKHVVNTILDTLGTNDFVNIFTFDKEVSPVVPCFEDTLIQANLGNIRELKEGIELFRPKSIANYTAALTKAFELLEETKLSSRGAQCNQAIMIIGDGAPENNREVFELHNWRDPPYKPVRVFTYLIGKEVANWDDIRWMACENQGYYVHLSDTAEVREMVLNYIPVMARPLVLGRHDHPVIWSQVYADIEDTKLSDYLWDINQCEYQKADVLEYWQVHDRMLEPSEMHRRKYRRMKETWNQPVDSNVYQFMTTVSMPIYDRRENATRIANILGVAGTDVPINEIKKLLSPFTLGVNGYAFIVTNNGYVLFHPDFRPIFQGYILKPAYNSVDMIEVELLDDDRPARDFNPVLMTIRDSIINQSTGSKWMLVKNHFDEMKRVARVKRQYYWTAIKKTPFTLVISYPEQYGVSHMDIRADQEIHRISIKGTNLRSVFSGKRWKIHPDWLFCKHSNRTFKTPEIELLYFLERMSEPGWRWPGSRSAMPPEHAAAMFCDRQLMQALVFDARVTGWFSNNTSFNSKDDKGNEFKQRFGVTVAFLATHSGLTRWHEFHSNAAEESGVGETFSQNNTRAIDEIWYKRAVDQHFVREESFVYSVPFDAGESNSEILVTASHAVFHNEGGKTAPAAVVGFQFQHSALYKLFHNITGNACAVDDKDCYILDNNGYVIISTRVHETGRFFGEVNGAIMKRLLEENVYRQVTVYDYQAVCFESKNDNNASSMLLSPLFHLLRVGKWLLHTALWYIVQLLQWAPGVSSHYADMYGDSNDTEPPPPEPHPDHHARNGNGHGKKDDDHWLRYLTLHRTRLKPCDMKRDLYTLFNEKDNVVYNMTAHACERPFVVLPIPFSNLILLVIDQLCPRDGSVVLTVNPQPIDYHLSVNDSLACYKQAREFNRMRPHSCISRHANESGIKLCGKACSVYANLGLLLLCHILSRWL

Target ORF information:

RefSeq Version	NM_137058.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster straightjacket (stj), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_137058.4

ORF Insert Sequence:

ATGGCCTGGT CCCGTCTCCT GGCCTGGTGG CGCCTCTGCT TGGGCCTGCT CTCCGTTCTG 
GTCTGTTTGG CACCGTGCGT CAATCTGCAG CCAGCCGAGA ACATCAACTA CAATCTCGTT 
CATTCGTGGG CCGACAAATT GGGCATGGAG CTGTTTCATC TGGGCGATTT CATCACCAGG 
CGCAAGGAGG TGCAAGAGAG TTTCAAGGAT GCAAAGGTCG TTTCGCGCAA CGGCGCTTCT 
ATTGTCGATT CGATGGCCAA GGAAATCGAA ATGATGATGG ACCTAAAAGT CAGTGCAGTG 
AGGCGAATCA TGGACACGGC TGAGAACACG GCGTTGTCGC ATCAGAACGA CATGGCGGAC 
AAGATGTTCT CCTATTATAG TGCCAAGGAG ATGCTGGAGC CCGGCGATCC AGTCCCGCCC 
ATTCCCACAC CGGCTCCCGA CATGGACAAG GATATAGGCG AGCCGCTGAT CTACGTCCAG 
CCGAAGGTGG TGGTGTTGGA ACCGCGTCCG GAATTCCACA ATACTCCGGT CAACTTTAGC 
GTCAGTTCGG TCCACGTGCC AGTCAATGTG TTCGATCGAG CACCGGATGT GATAAAAGCA 
ATTCAGTGGT CGGAGAATTT GGATCAGATA TTCCGTGACA ATTACAAAAA CGATCCGACA 
TTGTCGTGGC AATTCTTTGG CAGTTCGACT GGCTTTATGC GTCAATTCCC TGCATCGAAG 
TGGCGAAAGG ACGTACCGGT CGATCTATAC GATTGCCGAC TACGCTCCTG GTACATGGAG 
GCGGCGACGA GTCCGAAGGA TATTGTTATC CTGATGGATG GATCCGGTTC AATGTTGGGA 
CAGAGGCTAG ATATCGCAAA GCATGTTGTC AATACGATAC TCGATACATT AGGTACAAAT 
GACTTTGTGA ACATCTTCAC CTTTGATAAG GAAGTGAGCC CAGTCGTCCC ATGTTTCGAA 
GACACACTGA TTCAAGCCAA TCTGGGCAAC ATACGCGAAC TGAAAGAAGG GATTGAACTG 
TTTAGACCCA AATCGATCGC CAATTATACC GCTGCATTGA CAAAGGCATT CGAGCTATTG 
GAAGAGACCA AGTTGAGTTC GCGCGGGGCG CAGTGCAATC AGGCGATCAT GATCATTGGC 
GACGGGGCGC CGGAGAACAA TCGCGAGGTC TTCGAGTTGC ACAATTGGCG GGACCCGCCG 
TACAAGCCGG TCCGTGTGTT CACCTACCTG ATTGGCAAGG AGGTGGCCAA TTGGGATGAT 
ATACGGTGGA TGGCGTGCGA GAATCAGGGC TACTACGTCC ACCTCAGCGA CACCGCCGAG 
GTGCGCGAGA TGGTCTTGAA TTACATACCG GTTATGGCCA GGCCGCTCGT CCTGGGCAGG 
CACGATCATC CGGTGATTTG GTCGCAGGTC TATGCGGACA TCGAGGACAC GAAGCTATCC 
GACTATCTGT GGGACATTAA TCAGTGCGAG TACCAGAAGG CCGATGTCCT CGAGTATTGG 
CAGGTGCACG ATCGGATGCT GGAGCCCAGC GAAATGCACC GCCGGAAGTA CAGAAGGATG 
AAGGAGACCT GGAACCAGCC GGTGGACTCG AATGTGTACC AATTTATGAC CACCGTATCG 
ATGCCCATCT ACGATCGTCG CGAGAACGCG ACTAGGATTG CGAATATATT GGGCGTGGCC 
GGCACGGATG TGCCCATCAA TGAAATCAAA AAGCTGCTGT CGCCGTTCAC GCTCGGCGTG 
AATGGCTATG CGTTTATAGT AACCAACAAT GGTTATGTAC TATTCCATCC AGACTTTCGT 
CCAATTTTCC AAGGCTACAT ATTAAAGCCA GCATACAATA GCGTTGACAT GATTGAAGTC 
GAGCTATTGG ATGACGATCG ACCCGCCAGA GACTTTAATC CAGTGCTGAT GACGATAAGA 
GATTCTATAA TCAATCAATC GACAGGCAGC AAATGGATGT TAGTCAAAAA TCATTTCGAT 
GAAATGAAAC GGGTGGCACG CGTAAAGCGA CAATACTACT GGACGGCCAT CAAGAAGACG 
CCCTTTACTC TGGTCATTTC CTATCCGGAG CAATACGGCG TGAGTCACAT GGACATCCGA 
GCTGACCAGG AGATCCATCG CATAAGCATA AAGGGCACAA ACTTGCGCAG CGTTTTCAGC 
GGCAAGCGGT GGAAAATTCA TCCCGACTGG CTATTTTGTA AGCACAGCAA CAGGACATTC 
AAAACGCCAG AGATCGAACT GCTGTACTTT CTCGAGCGTA TGTCTGAGCC TGGCTGGCGA 
TGGCCGGGTA GTCGGAGTGC TATGCCCCCG GAGCACGCGG CCGCCATGTT TTGCGATCGG 
CAGCTAATGC AGGCTCTGGT CTTCGATGCC CGAGTCACCG GGTGGTTCTC CAACAATACC 
AGCTTTAACT CCAAGGACGA CAAAGGAAAC GAATTCAAGC AGCGTTTTGG CGTCACCGTC 
GCCTTTTTGG CCACCCACAG TGGTCTCACC CGCTGGCATG AGTTCCACTC GAATGCTGCC 
GAGGAATCCG GAGTCGGCGA GACTTTTAGC CAAAATAACA CGCGGGCCAT CGATGAGATT 
TGGTACAAAC GCGCCGTGGA CCAACATTTT GTGCGGGAGG AGAGCTTCGT TTACTCCGTG 
CCCTTCGATG CGGGTGAAAG CAACTCAGAG ATCCTGGTGA CCGCCAGTCA TGCCGTTTTC 
CACAATGAGG GCGGCAAGAC TGCACCGGCG GCTGTTGTGG GCTTCCAGTT TCAGCATTCG 
GCGCTCTACA AGCTCTTCCA CAACATCACC GGCAATGCCT GTGCCGTGGA TGACAAAGAC 
TGCTATATAC TGGACAACAA TGGCTATGTG ATCATTTCGA CGAGGGTCCA CGAGACGGGT 
CGATTCTTTG GCGAGGTGAA CGGAGCGATC ATGAAGCGAC TACTGGAGGA GAATGTCTAC 
AGGCAGGTCA CTGTCTACGA TTACCAGGCG GTGTGCTTCG AGTCCAAGAA CGATAACAAC 
GCCTCCAGCA TGCTGCTTTC GCCCCTGTTC CATCTGCTGC GCGTGGGCAA ATGGCTGCTG 
CATACGGCGC TGTGGTATAT TGTACAACTG TTGCAGTGGG CGCCCGGTGT GTCCAGCCAC 
TATGCGGATA TGTATGGTGA CTCCAACGAC ACGGAGCCAC CGCCGCCGGA GCCGCATCCG 
GATCACCATG CTCGCAACGG CAATGGGCAT GGGAAGAAGG ACGATGACCA CTGGCTGCGC 
TACTTGACAC TCCATCGCAC CCGGCTCAAG CCGTGCGACA TGAAGCGCGA TCTGTACACG 
CTGTTCAATG AAAAGGACAA CGTTGTCTAC AATATGACAG CCCATGCATG TGAGCGGCCA 
TTTGTCGTCC TGCCCATCCC GTTCAGCAAC CTCATCCTGC TGGTCATCGA CCAGCTGTGT 
CCCAGGGACG GCTCAGTCGT CCTCACGGTG AATCCGCAGC CGATCGACTA TCATCTGTCG 
GTCAACGATT CGTTGGCATG CTACAAGCAG GCTCGCGAGT TCAACCGCAT GCGACCACAC 
AGCTGCATCA GTCGGCACGC GAACGAGAGC GGCATTAAGC TGTGCGGAAA GGCCTGCTCC 
GTGTACGCCA ACCTGGGGCT GCTGCTCCTC TGTCACATAC TGAGTCGTTG GCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

stj ( NM_001259375.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246304.1 Drosophila melanogaster straightjacket (stj), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa13014
Clone ID Related Accession (Same CDS sequence)	NM_001259375.2
Accession Version	NM_001259375.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3714bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	straightjacket, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001259375.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCTGGT CCCGTCTCCT GGCCTGGTGG CGCCTCTGCT TGGGCCTGCT CTCCGTTCTG 
GTCTGTTTGG CACCGTGCGT CAATCTGCAG CCAGCCGAGA ACATCAACTA CAATCTCGTT 
CATTCGTGGG CCGACAAATT GGGCATGGAG CTGTTTCATC TGGGCGATTT CATCACCAGG 
CGCAAGGAGG TGCAAGAGAG TTTCAAGGAT GCAAAGGTCG TTTCGCGCAA CGGCGCTTCT 
ATTGTCGATT CGATGGCCAA GGAAATCGAA ATGATGATGG ACCTAAAAGT CAGTGCAGTG 
AGGCGAATCA TGGACACGGC TGAGAACACG GCGTTGTCGC ATCAGAACGA CATGGCGGAC 
AAGATGTTCT CCTATTATAG TGCCAAGGAG ATGCTGGAGC CCGGCGATCC AGTCCCGCCC 
ATTCCCACAC CGGCTCCCGA CATGGACAAG GATATAGGCG AGCCGCTGAT CTACGTCCAG 
CCGAAGGTGG TGGTGTTGGA ACCGCGTCCG GAATTCCACA ATACTCCGGT CAACTTTAGC 
GTCAGTTCGG TCCACGTGCC AGTCAATGTG TTCGATCGAG CACCGGATGT GATAAAAGCA 
ATTCAGTGGT CGGAGAATTT GGATCAGATA TTCCGTGACA ATTACAAAAA CGATCCGACA 
TTGTCGTGGC AATTCTTTGG CAGTTCGACT GGCTTTATGC GTCAATTCCC TGCATCGAAG 
TGGCGAAAGG ACGTACCGGT CGATCTATAC GATTGCCGAC TACGCTCCTG GTACATGGAG 
GCGGCGACGA GTCCGAAGGA TATTGTTATC CTGATGGATG GATCCGGTTC AATGTTGGGA 
CAGAGGCTAG ATATCGCAAA GCATGTTGTC AATACGATAC TCGATACATT AGGTACAAAT 
GACTTTGTGA ACATCTTCAC CTTTGATAAG GAAGTGAGCC CAGTCGTCCC ATGTTTCGAA 
GACACACTGA TTCAAGCCAA TCTGGGCAAC ATACGCGAAC TGAAAGAAGG GATTGAACTG 
TTTAGACCCA AATCGATCGC CAATTATACC GCTGCATTGA CAAAGGCATT CGAGCTATTG 
GAAGAGACCA AGTTGAGTTC GCGCGGGGCG CAGTGCAATC AGGCGATCAT GATCATTGGC 
GACGGGGCGC CGGAGAACAA TCGCGAGGTC TTCGAGTTGC ACAATTGGCG GGACCCGCCG 
TACAAGCCGG TCCGTGTGTT CACCTACCTG ATTGGCAAGG AGGTGGCCAA TTGGGATGAT 
ATACGGTGGA TGGCGTGCGA GAATCAGGGC TACTACGTCC ACCTCAGCGA CACCGCCGAG 
GTGCGCGAGA TGGTCTTGAA TTACATACCG GTTATGGCCA GGCCGCTCGT CCTGGGCAGG 
CACGATCATC CGGTGATTTG GTCGCAGGTC TATGCGGACA TCGAGGACAC GAAGCTATCC 
GACTATCTGT GGGACATTAA TCAGTGCGAG TACCAGAAGG CCGATGTCCT CGAGTATTGG 
CAGGTGCACG ATCGGATGCT GGAGCCCAGC GAAATGCACC GCCGGAAGTA CAGAAGGATG 
AAGGAGACCT GGAACCAGCC GGTGGACTCG AATGTGTACC AATTTATGAC CACCGTATCG 
ATGCCCATCT ACGATCGTCG CGAGAACGCG AATATCACCG AAGAAGTTTT AATAAATGAA 
GCACTCTGGG AATTGCAAAC ACGTGAGACT AGGATTGCGA ATATATTGGG CGTGGCCGGC 
ACGGATGTGC CCATCAATGA AATCAAAAAG CTGCTGTCGC CGTTCACGCT CGGCGTGAAT 
GGCTATGCGT TTATAGTAAC CAACAATGGT TATGTACTAT TCCATCCAGA CTTTCGTCCA 
ATTTTCCAAG GCTACATATT AAAGCCAGCA TACAATAGCG TTGACATGAT TGAAGTCGAG 
CTATTGGATG ACGATCGACC CGCCAGAGAC TTTAATCCAG TGCTGATGAC GATAAGAGAT 
TCTATAATCA ATCAATCGAC AGGCAGCAAA TGGATGTTAG TCAAAAATCA TTTCGATGAA 
ATGAAACGGG TGGCACGCGT AAAGCGACAA TACTACTGGA CGGCCATCAA GAAGACGCCC 
TTTACTCTGG TCATTTCCTA TCCGGAGCAA TACGGCGTGA GTCACATGGA CATCCGAGCT 
GACCAGGAGA TCCATCGCAT AAGCATAAAG GGCACAAACT TGCGCAGCGT TTTCAGCGGC 
AAGCGGTGGA AAATTCATCC CGACTGGCTA TTTTGTAAGC ACAGCAACAG GACATTCAAA 
ACGCCAGAGA TCGAACTGCT GTACTTTCTC GAGCGTATGT CTGAGCCTGG CTGGCGATGG 
CCGGGTAGTC GGAGTGCTAT GCCCCCGGAG CACGCGGCCG CCATGTTTTG CGATCGGCAG 
CTAATGCAGG CTCTGGTCTT CGATGCCCGA GTCACCGGGT GGTTCTCCAA CAATACCAGC 
TTTAACTCCA AGGACGACAA AGGAAACGAA TTCAAGCAGC GTTTTGGCGT CACCGTCGCC 
TTTTTGGCCA CCCACAGTGG TCTCACCCGC TGGCATGAGT TCCACTCGAA TGCTGCCGAG 
GAATCCGGAG TCGGCGAGAC TTTTAGCCAA AATAACACGC GGGCCATCGA TGAGATTTGG 
TACAAACGCG CCGTGGACCA ACATTTTGTG CGGGAGGAGA GCTTCGTTTA CTCCGTGCCC 
TTCGATGCGG GTGAAAGCAA CTCAGAGATC CTGGTGACCG CCAGTCATGC CGTTTTCCAC 
AATGAGGGCG GCAAGACTGC ACCGGCGGCT GTTGTGGGCT TCCAGTTTCA GCATTCGGCG 
CTCTACAAGC TCTTCCACAA CATCACCGGC AATGCCTGTG CCGTGGATGA CAAAGACTGC 
TATATACTGG ACAACAATGG CTATGTGATC ATTTCGACGA GGGTCCACGA GACGGGTCGA 
TTCTTTGGCG AGGTGAACGG AGCGATCATG AAGCGACTAC TGGAGGAGAA TGTCTACAGG 
CAGGTCACTG TCTACGATTA CCAGGCGGTG TGCTTCGAGT CCAAGAACGA TAACAACGCC 
TCCAGCATGC TGCTTTCGCC CCTGTTCCAT CTGCTGCGCG TGGGCAAATG GCTGCTGCAT 
ACGGCGCTGT GGTATATTGT ACAACTGTTG CAGTGGGCGC CCGGTGTGTC CAGCCACTAT 
GCGGATATGT ATGGTGACTC CAACGACACG GAGCCACCGC CGCCGGAGCC GCATCCGGAT 
CACCATGCTC GCAACGGCAA TGGGCATGGG AAGAAGGACG ATGACCACTG GCTGCGCTAC 
TTGACACTCC ATCGCACCCG GCTCAAGCCG TGCGACATGA AGCGCGATCT GTACACGCTG 
TTCAATGAAA AGGACAACGT TGTCTACAAT ATGACAGCCC ATGCATGTGA GCGGCCATTT 
GTCGTCCTGC CCATCCCGTT CAGCAACCTC ATCCTGCTGG TCATCGACCA GCTGTGTCCC 
AGGGACGGCT CAGTCGTCCT CACGGTGAAT CCGCAGCCGA TCGACTATCA TCTGTCGGTC 
AACGATTCGT TGGCATGCTA CAAGCAGGCT CGCGAGTTCA ACCGCATGCG ACCACACAGC 
TGCATCAGTC GGCACGCGAA CGAGAGCGGC ATTAAGCTGT GCGGAAAGGC CTGCTCCGTG 
TACGCCAACC TGGGGCTGCT GCTCCTCTGT CACATACTGA GTCGTTGGCT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246304.1
CDS	810..4523
Translation	MAWSRLLAWWRLCLGLLSVLVCLAPCVNLQPAENINYNLVHSWADKLGMELFHLGDFITRRKEVQESFKDAKVVSRNGASIVDSMAKEIEMMMDLKVSAVRRIMDTAENTALSHQNDMADKMFSYYSAKEMLEPGDPVPPIPTPAPDMDKDIGEPLIYVQPKVVVLEPRPEFHNTPVNFSVSSVHVPVNVFDRAPDVIKAIQWSENLDQIFRDNYKNDPTLSWQFFGSSTGFMRQFPASKWRKDVPVDLYDCRLRSWYMEAATSPKDIVILMDGSGSMLGQRLDIAKHVVNTILDTLGTNDFVNIFTFDKEVSPVVPCFEDTLIQANLGNIRELKEGIELFRPKSIANYTAALTKAFELLEETKLSSRGAQCNQAIMIIGDGAPENNREVFELHNWRDPPYKPVRVFTYLIGKEVANWDDIRWMACENQGYYVHLSDTAEVREMVLNYIPVMARPLVLGRHDHPVIWSQVYADIEDTKLSDYLWDINQCEYQKADVLEYWQVHDRMLEPSEMHRRKYRRMKETWNQPVDSNVYQFMTTVSMPIYDRRENANITEEVLINEALWELQTRETRIANILGVAGTDVPINEIKKLLSPFTLGVNGYAFIVTNNGYVLFHPDFRPIFQGYILKPAYNSVDMIEVELLDDDRPARDFNPVLMTIRDSIINQSTGSKWMLVKNHFDEMKRVARVKRQYYWTAIKKTPFTLVISYPEQYGVSHMDIRADQEIHRISIKGTNLRSVFSGKRWKIHPDWLFCKHSNRTFKTPEIELLYFLERMSEPGWRWPGSRSAMPPEHAAAMFCDRQLMQALVFDARVTGWFSNNTSFNSKDDKGNEFKQRFGVTVAFLATHSGLTRWHEFHSNAAEESGVGETFSQNNTRAIDEIWYKRAVDQHFVREESFVYSVPFDAGESNSEILVTASHAVFHNEGGKTAPAAVVGFQFQHSALYKLFHNITGNACAVDDKDCYILDNNGYVIISTRVHETGRFFGEVNGAIMKRLLEENVYRQVTVYDYQAVCFESKNDNNASSMLLSPLFHLLRVGKWLLHTALWYIVQLLQWAPGVSSHYADMYGDSNDTEPPPPEPHPDHHARNGNGHGKKDDDHWLRYLTLHRTRLKPCDMKRDLYTLFNEKDNVVYNMTAHACERPFVVLPIPFSNLILLVIDQLCPRDGSVVLTVNPQPIDYHLSVNDSLACYKQAREFNRMRPHSCISRHANESGIKLCGKACSVYANLGLLLLCHILSRWL

Target ORF information:

RefSeq Version	NM_001259375.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster straightjacket (stj), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259375.2

ORF Insert Sequence:

ATGGCCTGGT CCCGTCTCCT GGCCTGGTGG CGCCTCTGCT TGGGCCTGCT CTCCGTTCTG 
GTCTGTTTGG CACCGTGCGT CAATCTGCAG CCAGCCGAGA ACATCAACTA CAATCTCGTT 
CATTCGTGGG CCGACAAATT GGGCATGGAG CTGTTTCATC TGGGCGATTT CATCACCAGG 
CGCAAGGAGG TGCAAGAGAG TTTCAAGGAT GCAAAGGTCG TTTCGCGCAA CGGCGCTTCT 
ATTGTCGATT CGATGGCCAA GGAAATCGAA ATGATGATGG ACCTAAAAGT CAGTGCAGTG 
AGGCGAATCA TGGACACGGC TGAGAACACG GCGTTGTCGC ATCAGAACGA CATGGCGGAC 
AAGATGTTCT CCTATTATAG TGCCAAGGAG ATGCTGGAGC CCGGCGATCC AGTCCCGCCC 
ATTCCCACAC CGGCTCCCGA CATGGACAAG GATATAGGCG AGCCGCTGAT CTACGTCCAG 
CCGAAGGTGG TGGTGTTGGA ACCGCGTCCG GAATTCCACA ATACTCCGGT CAACTTTAGC 
GTCAGTTCGG TCCACGTGCC AGTCAATGTG TTCGATCGAG CACCGGATGT GATAAAAGCA 
ATTCAGTGGT CGGAGAATTT GGATCAGATA TTCCGTGACA ATTACAAAAA CGATCCGACA 
TTGTCGTGGC AATTCTTTGG CAGTTCGACT GGCTTTATGC GTCAATTCCC TGCATCGAAG 
TGGCGAAAGG ACGTACCGGT CGATCTATAC GATTGCCGAC TACGCTCCTG GTACATGGAG 
GCGGCGACGA GTCCGAAGGA TATTGTTATC CTGATGGATG GATCCGGTTC AATGTTGGGA 
CAGAGGCTAG ATATCGCAAA GCATGTTGTC AATACGATAC TCGATACATT AGGTACAAAT 
GACTTTGTGA ACATCTTCAC CTTTGATAAG GAAGTGAGCC CAGTCGTCCC ATGTTTCGAA 
GACACACTGA TTCAAGCCAA TCTGGGCAAC ATACGCGAAC TGAAAGAAGG GATTGAACTG 
TTTAGACCCA AATCGATCGC CAATTATACC GCTGCATTGA CAAAGGCATT CGAGCTATTG 
GAAGAGACCA AGTTGAGTTC GCGCGGGGCG CAGTGCAATC AGGCGATCAT GATCATTGGC 
GACGGGGCGC CGGAGAACAA TCGCGAGGTC TTCGAGTTGC ACAATTGGCG GGACCCGCCG 
TACAAGCCGG TCCGTGTGTT CACCTACCTG ATTGGCAAGG AGGTGGCCAA TTGGGATGAT 
ATACGGTGGA TGGCGTGCGA GAATCAGGGC TACTACGTCC ACCTCAGCGA CACCGCCGAG 
GTGCGCGAGA TGGTCTTGAA TTACATACCG GTTATGGCCA GGCCGCTCGT CCTGGGCAGG 
CACGATCATC CGGTGATTTG GTCGCAGGTC TATGCGGACA TCGAGGACAC GAAGCTATCC 
GACTATCTGT GGGACATTAA TCAGTGCGAG TACCAGAAGG CCGATGTCCT CGAGTATTGG 
CAGGTGCACG ATCGGATGCT GGAGCCCAGC GAAATGCACC GCCGGAAGTA CAGAAGGATG 
AAGGAGACCT GGAACCAGCC GGTGGACTCG AATGTGTACC AATTTATGAC CACCGTATCG 
ATGCCCATCT ACGATCGTCG CGAGAACGCG AATATCACCG AAGAAGTTTT AATAAATGAA 
GCACTCTGGG AATTGCAAAC ACGTGAGACT AGGATTGCGA ATATATTGGG CGTGGCCGGC 
ACGGATGTGC CCATCAATGA AATCAAAAAG CTGCTGTCGC CGTTCACGCT CGGCGTGAAT 
GGCTATGCGT TTATAGTAAC CAACAATGGT TATGTACTAT TCCATCCAGA CTTTCGTCCA 
ATTTTCCAAG GCTACATATT AAAGCCAGCA TACAATAGCG TTGACATGAT TGAAGTCGAG 
CTATTGGATG ACGATCGACC CGCCAGAGAC TTTAATCCAG TGCTGATGAC GATAAGAGAT 
TCTATAATCA ATCAATCGAC AGGCAGCAAA TGGATGTTAG TCAAAAATCA TTTCGATGAA 
ATGAAACGGG TGGCACGCGT AAAGCGACAA TACTACTGGA CGGCCATCAA GAAGACGCCC 
TTTACTCTGG TCATTTCCTA TCCGGAGCAA TACGGCGTGA GTCACATGGA CATCCGAGCT 
GACCAGGAGA TCCATCGCAT AAGCATAAAG GGCACAAACT TGCGCAGCGT TTTCAGCGGC 
AAGCGGTGGA AAATTCATCC CGACTGGCTA TTTTGTAAGC ACAGCAACAG GACATTCAAA 
ACGCCAGAGA TCGAACTGCT GTACTTTCTC GAGCGTATGT CTGAGCCTGG CTGGCGATGG 
CCGGGTAGTC GGAGTGCTAT GCCCCCGGAG CACGCGGCCG CCATGTTTTG CGATCGGCAG 
CTAATGCAGG CTCTGGTCTT CGATGCCCGA GTCACCGGGT GGTTCTCCAA CAATACCAGC 
TTTAACTCCA AGGACGACAA AGGAAACGAA TTCAAGCAGC GTTTTGGCGT CACCGTCGCC 
TTTTTGGCCA CCCACAGTGG TCTCACCCGC TGGCATGAGT TCCACTCGAA TGCTGCCGAG 
GAATCCGGAG TCGGCGAGAC TTTTAGCCAA AATAACACGC GGGCCATCGA TGAGATTTGG 
TACAAACGCG CCGTGGACCA ACATTTTGTG CGGGAGGAGA GCTTCGTTTA CTCCGTGCCC 
TTCGATGCGG GTGAAAGCAA CTCAGAGATC CTGGTGACCG CCAGTCATGC CGTTTTCCAC 
AATGAGGGCG GCAAGACTGC ACCGGCGGCT GTTGTGGGCT TCCAGTTTCA GCATTCGGCG 
CTCTACAAGC TCTTCCACAA CATCACCGGC AATGCCTGTG CCGTGGATGA CAAAGACTGC 
TATATACTGG ACAACAATGG CTATGTGATC ATTTCGACGA GGGTCCACGA GACGGGTCGA 
TTCTTTGGCG AGGTGAACGG AGCGATCATG AAGCGACTAC TGGAGGAGAA TGTCTACAGG 
CAGGTCACTG TCTACGATTA CCAGGCGGTG TGCTTCGAGT CCAAGAACGA TAACAACGCC 
TCCAGCATGC TGCTTTCGCC CCTGTTCCAT CTGCTGCGCG TGGGCAAATG GCTGCTGCAT 
ACGGCGCTGT GGTATATTGT ACAACTGTTG CAGTGGGCGC CCGGTGTGTC CAGCCACTAT 
GCGGATATGT ATGGTGACTC CAACGACACG GAGCCACCGC CGCCGGAGCC GCATCCGGAT 
CACCATGCTC GCAACGGCAA TGGGCATGGG AAGAAGGACG ATGACCACTG GCTGCGCTAC 
TTGACACTCC ATCGCACCCG GCTCAAGCCG TGCGACATGA AGCGCGATCT GTACACGCTG 
TTCAATGAAA AGGACAACGT TGTCTACAAT ATGACAGCCC ATGCATGTGA GCGGCCATTT 
GTCGTCCTGC CCATCCCGTT CAGCAACCTC ATCCTGCTGG TCATCGACCA GCTGTGTCCC 
AGGGACGGCT CAGTCGTCCT CACGGTGAAT CCGCAGCCGA TCGACTATCA TCTGTCGGTC 
AACGATTCGT TGGCATGCTA CAAGCAGGCT CGCGAGTTCA ACCGCATGCG ACCACACAGC 
TGCATCAGTC GGCACGCGAA CGAGAGCGGC ATTAAGCTGT GCGGAAAGGC CTGCTCCGTG 
TACGCCAACC TGGGGCTGCT GCTCCTCTGT CACATACTGA GTCGTTGGCT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

stj ( NM_001259374.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246303.1 Drosophila melanogaster straightjacket (stj), transcript variant C, mRNA.

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CloneID	OFa13015
Clone ID Related Accession (Same CDS sequence)	NM_001259374.2
Accession Version	NM_001259374.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3816bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	straightjacket, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001259374.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCTGGT CCCGTCTCCT GGCCTGGTGG CGCCTCTGCT TGGGCCTGCT CTCCGTTCTG 
GTCTGTTTGG CACCGTGCGT CAATCTGCAG CCAGCCGAGA ACATCAACTA CAATCTCGTT 
CATTCGTGGG CCGACAAATT GGGCATGGAG CTGTTTCATC TGGGCGATTT CATCACCAGG 
CGCAAGGAGG TGCAAGAGAG TTTCAAGGAT GCAAAGGTCG TTTCGCGCAA CGGCGCTTCT 
ATTGTCGATT CGATGGCCAA GGAAATCGAA ATGATGATGG ACCTAAAAGT CAGTGCAGTG 
AGGCGAATCA TGGACACGGC TGAGAACACG GCGTTGTCGC ATCAGAACGA CATGGCGGAC 
AAGATGTTCT CCTATTATAG TGCCAAGGAG ATGCTGGAGC CCGGCGATCC AGTCCCGCCC 
ATTCCCACAC CGGCTCCCGA CATGGACAAG GATATAGGCG AGCCGCTGAT CTACGTCCAG 
CCGAAGGTGG TGGTGTTGGA ACCGCGTCCG GAATTCCACA ATACTCCGGT CAACTTTAGC 
GTCAGTTCGG TCCACGTGCC AGTCAATGTG TTCGATCGAG CACCGGATGT GATAAAAGCA 
ATTCAGTGGT CGGAGAATTT GGATCAGATA TTCCGTGACA ATTACAAAAA CGATCCGACA 
TTGTCGTGGC AATTCTTTGG CAGTTCGACT GGCTTTATGC GTCAATTCCC TGCATCGAAG 
TGGCGAAAGG ACGTACCGGT CGATCTATAC GATTGCCGAC TACGCTCCTG GTACATGGAG 
GCGGCGACGA GTCCGAAGGA TATTGTTATC CTGATGGATG GATCCGGTTC AATGTTGGGA 
CAGAGGCTAG ATATCGCAAA GCATGTTGTC AATACGATAC TCGATACATT AGGTACAAAT 
GACTTTGTGA ACATCTTCAC CTTTGATAAG GAAGTGAGCC CAGTCGTCCC ATGTTTCGAA 
GACACACTGA TTCAAGCCAA TCTGGGCAAC ATACGCGAAC TGAAAGAAGG GATTGAACTG 
TTTAGACCCA AATCGATCGC CAATTATACC GCTGCATTGA CAAAGGCATT CGAGCTATTG 
GAAGAGACCA AGTTGAGTTC GCGCGGGGCG CAGTGCAATC AGGCGATCAT GATCATTGGC 
GACGGGGCGC CGGAGAACAA TCGCGAGGTC TTCGAGTTGC ACAATTGGCG GGACCCGCCG 
TACAAGCCGG TCCGTGTGTT CACCTACCTG ATTGGCAAGG AGGTGGCCAA TTGGGATGAT 
ATACGGTGGA TGGCGTGCGA GAATCAGGGC TACTACGTCC ACCTCAGCGA CACCGCCGAG 
GTGCGCGAGA TGGTCTTGAA TTACATACCG GTTATGGCCA GGCCGCTCGT CCTGGGCAGG 
CACGATCATC CGGTGATTTG GTCGCAGGTC TATGCGGACA TCGAGGACAC GAAGCTATCC 
GACTATCTGT GGGACATTAA TCAGTGCGAG TACCAGAAGG CCGATGTCCT CGAGTATTGG 
CAGGTGCACG ATCGGATGCT GGAGCCCAGC GAAATGCACC GCCGGAAGTA CAGAAGGATG 
AAGGAGACCT GGAACCAGCC GGTGGACTCG AATGTGTACC AATTTATGAC CACCGTATCG 
ATGCCCATCT ACGATCGTCG CGAGAACGCG CTCATTAATC TAACGACCGA TATAAATCCG 
GCTGCGAAAA TAAACCTTCA AGTTACTAGG ATTGCGAATA TATTGGGCGT GGCCGGCACG 
GATGTGCCCA TCAATGAAAT CAAAAAGCTG CTGTCGCCGT TCACGCTCGG CGTGAATGGC 
TATGCGTTTA TAGTAACCAA CAATGGTTAT GTACTATTCC ATCCAGACTT TCGTCCAATT 
TTCCAAGGCT ACATATTAAA GCCAGCATAC AATAGCGTTG ACATGATTGA AGTCGAGCTA 
TTGGATGACG ATCGACCCGC CAGAGACTTT AATCCAGTGC TGATGACGAT AAGAGATTCT 
ATAATCAATC AATCGACAGG CAGCAAATGG ATGTTAGTCA AAAATCATTT CGATGAAATG 
AAACGGGTGG CACGCGTAAA GCGACAATAC TACTGGACGG CCATCAAGAA GACGCCCTTT 
ACTCTGGTCA TTTCCTATCC GGAGCAATAC GGCGTGAGTC ACATGGACAT CCGAGCTGAC 
CAGGAGATCC ATCGCATAAG CATAAAGGGC ACAAACTTGC GCAGCGTTTT CAGCGGCAAG 
CGGTGGAAAA TTCATCCCGA CTGGCTATTT TGTAAGCACA GCAACAGGAC ATTCAAAACG 
CCAGAGATCG AACTGCTGTA CTTTCTCGAG CGTATGTCTG AGCCTGGCTG GCGATGGCCG 
GGTAGTCGGA GTGCTATGCC CCCGGAGCAC GCGGCCGCCA TGTTTTCTAA CAACTCATCA 
ACCGGCCGTT ATCCATCAAT CAATGAAAAA GAAAGCTACT ATTGCGATCG GCAGCTAATG 
CAGGCTCTGG TCTTCGATGC CCGAGTCACC GGGTGGTTCT CCAACAATAC CAGCTTTAAC 
TCCAAGGACG ACAAAGGCAC AAGCGCATCA AGCCCAATAG CTGTTCTAAT GGGTTTGCTG 
CCAAGAAACG AATTCAAGCA GCGTTTTGGC GTCACCGTCG CCTTTTTGGC CACCCACAGT 
GGTCTCACCC GCTGGCATGA GTTCCACTCG AATGCTGCCG AGGAATCCGG AGTCGGCGAG 
ACTTTTAGCC AAAATAACAC GCGGGCCATC GATGAGATTT GGTACAAACG CGCCGTGGAC 
CAACATTTTG TGCGGGAGGA GAGCTTCGTT TACTCCGTGC CCTTCGATGC GGGTGAAAGC 
AACTCAGAGA TCCTGGTGAC CGCCAGTCAT GCCGTTTTCC ACAATGAGGG CGGCAAGACT 
GCACCGGCGG CTGTTGTGGG CTTCCAGTTT CAGCATTCGG CGCTCTACAA GCTCTTCCAC 
AACATCACCG GCAATGCCTG TGCCGTGGAT GACAAAGACT GCTATATACT GGACAACAAT 
GGCTATGTGA TCATTTCGAC GAGGGTCCAC GAGACGGGTC GATTCTTTGG CGAGGTGAAC 
GGAGCGATCA TGAAGCGACT ACTGGAGGAG AATGTCTACA GGCAGGTCAC TGTCTACGAT 
TACCAGGCGG TGTGCTTCGA GTCCAAGAAC GATAACAACG CCTCCAGCAT GCTGCTTTCG 
CCCCTGTTCC ATCTGCTGCG CGTGGGCAAA TGGCTGCTGC ATACGGCGCT GTGGTATATT 
GTACAACTGT TGCAGTGGGC GCCCGGTGTG TCCAGCCACT ATGCGGATAT GTATGGTGAC 
TCCAACGACA CGGAGCCACC GCCGCCGGAG CCGCATCCGG ATCACCATGC TCGCAACGGC 
AATGGGCATG GGAAGAAGGA CGATGACCAC TGGCTGCGCT ACTTGACACT CCATCGCACC 
CGGCTCAAGC CGTGCGACAT GAAGCGCGAT CTGTACACGC TGTTCAATGA AAAGGACAAC 
GTTGTCTACA ATATGACAGC CCATGCATGT GAGCGGCCAT TTGTCGTCCT GCCCATCCCG 
TTCAGCAACC TCATCCTGCT GGTCATCGAC CAGCTGTGTC CCAGGGACGG CTCAGTCGTC 
CTCACGGTGA ATCCGCAGCC GATCGACTAT CATCTGTCGG TCAACGATTC GTTGGCATGC 
TACAAGCAGG CTCGCGAGTT CAACCGCATG CGACCACACA GCTGCATCAG TCGGCACGCG 
AACGAGAGCG GCATTAAGCT GTGCGGAAAG GCCTGCTCCG TGTACGCCAA CCTGGGGCTG 
CTGCTCCTCT GTCACATACT GAGTCGTTGG CTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246303.1
CDS	419..4234
Translation	MAWSRLLAWWRLCLGLLSVLVCLAPCVNLQPAENINYNLVHSWADKLGMELFHLGDFITRRKEVQESFKDAKVVSRNGASIVDSMAKEIEMMMDLKVSAVRRIMDTAENTALSHQNDMADKMFSYYSAKEMLEPGDPVPPIPTPAPDMDKDIGEPLIYVQPKVVVLEPRPEFHNTPVNFSVSSVHVPVNVFDRAPDVIKAIQWSENLDQIFRDNYKNDPTLSWQFFGSSTGFMRQFPASKWRKDVPVDLYDCRLRSWYMEAATSPKDIVILMDGSGSMLGQRLDIAKHVVNTILDTLGTNDFVNIFTFDKEVSPVVPCFEDTLIQANLGNIRELKEGIELFRPKSIANYTAALTKAFELLEETKLSSRGAQCNQAIMIIGDGAPENNREVFELHNWRDPPYKPVRVFTYLIGKEVANWDDIRWMACENQGYYVHLSDTAEVREMVLNYIPVMARPLVLGRHDHPVIWSQVYADIEDTKLSDYLWDINQCEYQKADVLEYWQVHDRMLEPSEMHRRKYRRMKETWNQPVDSNVYQFMTTVSMPIYDRRENALINLTTDINPAAKINLQVTRIANILGVAGTDVPINEIKKLLSPFTLGVNGYAFIVTNNGYVLFHPDFRPIFQGYILKPAYNSVDMIEVELLDDDRPARDFNPVLMTIRDSIINQSTGSKWMLVKNHFDEMKRVARVKRQYYWTAIKKTPFTLVISYPEQYGVSHMDIRADQEIHRISIKGTNLRSVFSGKRWKIHPDWLFCKHSNRTFKTPEIELLYFLERMSEPGWRWPGSRSAMPPEHAAAMFSNNSSTGRYPSINEKESYYCDRQLMQALVFDARVTGWFSNNTSFNSKDDKGTSASSPIAVLMGLLPRNEFKQRFGVTVAFLATHSGLTRWHEFHSNAAEESGVGETFSQNNTRAIDEIWYKRAVDQHFVREESFVYSVPFDAGESNSEILVTASHAVFHNEGGKTAPAAVVGFQFQHSALYKLFHNITGNACAVDDKDCYILDNNGYVIISTRVHETGRFFGEVNGAIMKRLLEENVYRQVTVYDYQAVCFESKNDNNASSMLLSPLFHLLRVGKWLLHTALWYIVQLLQWAPGVSSHYADMYGDSNDTEPPPPEPHPDHHARNGNGHGKKDDDHWLRYLTLHRTRLKPCDMKRDLYTLFNEKDNVVYNMTAHACERPFVVLPIPFSNLILLVIDQLCPRDGSVVLTVNPQPIDYHLSVNDSLACYKQAREFNRMRPHSCISRHANESGIKLCGKACSVYANLGLLLLCHILSRWL

Target ORF information:

RefSeq Version	NM_001259374.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster straightjacket (stj), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259374.2

ORF Insert Sequence:

ATGGCCTGGT CCCGTCTCCT GGCCTGGTGG CGCCTCTGCT TGGGCCTGCT CTCCGTTCTG 
GTCTGTTTGG CACCGTGCGT CAATCTGCAG CCAGCCGAGA ACATCAACTA CAATCTCGTT 
CATTCGTGGG CCGACAAATT GGGCATGGAG CTGTTTCATC TGGGCGATTT CATCACCAGG 
CGCAAGGAGG TGCAAGAGAG TTTCAAGGAT GCAAAGGTCG TTTCGCGCAA CGGCGCTTCT 
ATTGTCGATT CGATGGCCAA GGAAATCGAA ATGATGATGG ACCTAAAAGT CAGTGCAGTG 
AGGCGAATCA TGGACACGGC TGAGAACACG GCGTTGTCGC ATCAGAACGA CATGGCGGAC 
AAGATGTTCT CCTATTATAG TGCCAAGGAG ATGCTGGAGC CCGGCGATCC AGTCCCGCCC 
ATTCCCACAC CGGCTCCCGA CATGGACAAG GATATAGGCG AGCCGCTGAT CTACGTCCAG 
CCGAAGGTGG TGGTGTTGGA ACCGCGTCCG GAATTCCACA ATACTCCGGT CAACTTTAGC 
GTCAGTTCGG TCCACGTGCC AGTCAATGTG TTCGATCGAG CACCGGATGT GATAAAAGCA 
ATTCAGTGGT CGGAGAATTT GGATCAGATA TTCCGTGACA ATTACAAAAA CGATCCGACA 
TTGTCGTGGC AATTCTTTGG CAGTTCGACT GGCTTTATGC GTCAATTCCC TGCATCGAAG 
TGGCGAAAGG ACGTACCGGT CGATCTATAC GATTGCCGAC TACGCTCCTG GTACATGGAG 
GCGGCGACGA GTCCGAAGGA TATTGTTATC CTGATGGATG GATCCGGTTC AATGTTGGGA 
CAGAGGCTAG ATATCGCAAA GCATGTTGTC AATACGATAC TCGATACATT AGGTACAAAT 
GACTTTGTGA ACATCTTCAC CTTTGATAAG GAAGTGAGCC CAGTCGTCCC ATGTTTCGAA 
GACACACTGA TTCAAGCCAA TCTGGGCAAC ATACGCGAAC TGAAAGAAGG GATTGAACTG 
TTTAGACCCA AATCGATCGC CAATTATACC GCTGCATTGA CAAAGGCATT CGAGCTATTG 
GAAGAGACCA AGTTGAGTTC GCGCGGGGCG CAGTGCAATC AGGCGATCAT GATCATTGGC 
GACGGGGCGC CGGAGAACAA TCGCGAGGTC TTCGAGTTGC ACAATTGGCG GGACCCGCCG 
TACAAGCCGG TCCGTGTGTT CACCTACCTG ATTGGCAAGG AGGTGGCCAA TTGGGATGAT 
ATACGGTGGA TGGCGTGCGA GAATCAGGGC TACTACGTCC ACCTCAGCGA CACCGCCGAG 
GTGCGCGAGA TGGTCTTGAA TTACATACCG GTTATGGCCA GGCCGCTCGT CCTGGGCAGG 
CACGATCATC CGGTGATTTG GTCGCAGGTC TATGCGGACA TCGAGGACAC GAAGCTATCC 
GACTATCTGT GGGACATTAA TCAGTGCGAG TACCAGAAGG CCGATGTCCT CGAGTATTGG 
CAGGTGCACG ATCGGATGCT GGAGCCCAGC GAAATGCACC GCCGGAAGTA CAGAAGGATG 
AAGGAGACCT GGAACCAGCC GGTGGACTCG AATGTGTACC AATTTATGAC CACCGTATCG 
ATGCCCATCT ACGATCGTCG CGAGAACGCG CTCATTAATC TAACGACCGA TATAAATCCG 
GCTGCGAAAA TAAACCTTCA AGTTACTAGG ATTGCGAATA TATTGGGCGT GGCCGGCACG 
GATGTGCCCA TCAATGAAAT CAAAAAGCTG CTGTCGCCGT TCACGCTCGG CGTGAATGGC 
TATGCGTTTA TAGTAACCAA CAATGGTTAT GTACTATTCC ATCCAGACTT TCGTCCAATT 
TTCCAAGGCT ACATATTAAA GCCAGCATAC AATAGCGTTG ACATGATTGA AGTCGAGCTA 
TTGGATGACG ATCGACCCGC CAGAGACTTT AATCCAGTGC TGATGACGAT AAGAGATTCT 
ATAATCAATC AATCGACAGG CAGCAAATGG ATGTTAGTCA AAAATCATTT CGATGAAATG 
AAACGGGTGG CACGCGTAAA GCGACAATAC TACTGGACGG CCATCAAGAA GACGCCCTTT 
ACTCTGGTCA TTTCCTATCC GGAGCAATAC GGCGTGAGTC ACATGGACAT CCGAGCTGAC 
CAGGAGATCC ATCGCATAAG CATAAAGGGC ACAAACTTGC GCAGCGTTTT CAGCGGCAAG 
CGGTGGAAAA TTCATCCCGA CTGGCTATTT TGTAAGCACA GCAACAGGAC ATTCAAAACG 
CCAGAGATCG AACTGCTGTA CTTTCTCGAG CGTATGTCTG AGCCTGGCTG GCGATGGCCG 
GGTAGTCGGA GTGCTATGCC CCCGGAGCAC GCGGCCGCCA TGTTTTCTAA CAACTCATCA 
ACCGGCCGTT ATCCATCAAT CAATGAAAAA GAAAGCTACT ATTGCGATCG GCAGCTAATG 
CAGGCTCTGG TCTTCGATGC CCGAGTCACC GGGTGGTTCT CCAACAATAC CAGCTTTAAC 
TCCAAGGACG ACAAAGGCAC AAGCGCATCA AGCCCAATAG CTGTTCTAAT GGGTTTGCTG 
CCAAGAAACG AATTCAAGCA GCGTTTTGGC GTCACCGTCG CCTTTTTGGC CACCCACAGT 
GGTCTCACCC GCTGGCATGA GTTCCACTCG AATGCTGCCG AGGAATCCGG AGTCGGCGAG 
ACTTTTAGCC AAAATAACAC GCGGGCCATC GATGAGATTT GGTACAAACG CGCCGTGGAC 
CAACATTTTG TGCGGGAGGA GAGCTTCGTT TACTCCGTGC CCTTCGATGC GGGTGAAAGC 
AACTCAGAGA TCCTGGTGAC CGCCAGTCAT GCCGTTTTCC ACAATGAGGG CGGCAAGACT 
GCACCGGCGG CTGTTGTGGG CTTCCAGTTT CAGCATTCGG CGCTCTACAA GCTCTTCCAC 
AACATCACCG GCAATGCCTG TGCCGTGGAT GACAAAGACT GCTATATACT GGACAACAAT 
GGCTATGTGA TCATTTCGAC GAGGGTCCAC GAGACGGGTC GATTCTTTGG CGAGGTGAAC 
GGAGCGATCA TGAAGCGACT ACTGGAGGAG AATGTCTACA GGCAGGTCAC TGTCTACGAT 
TACCAGGCGG TGTGCTTCGA GTCCAAGAAC GATAACAACG CCTCCAGCAT GCTGCTTTCG 
CCCCTGTTCC ATCTGCTGCG CGTGGGCAAA TGGCTGCTGC ATACGGCGCT GTGGTATATT 
GTACAACTGT TGCAGTGGGC GCCCGGTGTG TCCAGCCACT ATGCGGATAT GTATGGTGAC 
TCCAACGACA CGGAGCCACC GCCGCCGGAG CCGCATCCGG ATCACCATGC TCGCAACGGC 
AATGGGCATG GGAAGAAGGA CGATGACCAC TGGCTGCGCT ACTTGACACT CCATCGCACC 
CGGCTCAAGC CGTGCGACAT GAAGCGCGAT CTGTACACGC TGTTCAATGA AAAGGACAAC 
GTTGTCTACA ATATGACAGC CCATGCATGT GAGCGGCCAT TTGTCGTCCT GCCCATCCCG 
TTCAGCAACC TCATCCTGCT GGTCATCGAC CAGCTGTGTC CCAGGGACGG CTCAGTCGTC 
CTCACGGTGA ATCCGCAGCC GATCGACTAT CATCTGTCGG TCAACGATTC GTTGGCATGC 
TACAAGCAGG CTCGCGAGTT CAACCGCATG CGACCACACA GCTGCATCAG TCGGCACGCG 
AACGAGAGCG GCATTAAGCT GTGCGGAAAG GCCTGCTCCG TGTACGCCAA CCTGGGGCTG 
CTGCTCCTCT GTCACATACT GAGTCGTTGG CTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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stj cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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