gnai3 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	gnai3
Entrez Gene ID	100006888
Full Name	guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 3
General protein information	Preferred Names guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 3 Names guanine nucleotide-binding protein G(k) subunit alpha Galpha i3
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001111250.1	NP_001104720.1	guanine nucleotide-binding protein G(k) subunit alpha

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	GNAI3	XP_513624.1
	GNAI3	XP_001091470.1
Bos taurus (cattle)	GNAI3	NP_001029849.1
Rattus norvegicus (Norway rat)	Gnai3	NP_037238.1
Canis lupus familiaris (dog)	GNAI3	XP_854614.3
Xenopus tropicalis (tropical clawed frog)	gnai3	NP_001011471.1
Homo sapiens (human)	GNAI3	NP_006487.1
Danio rerio (zebrafish)	gnai3	NP_001104720.1
Mus musculus (house mouse)	Gnai3	NP_034436.1
Gallus gallus (chicken)	GNAI3	NP_989580.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Distinct functions of two olfactory marker protein genes derived from teleost-specific whole genome duplication.
Suzuki H, Nikaido M, Hagino-Yamagishi K, Okada N
BMC evolutionary biology15245(2015 Nov)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

The vertebrate ancestral repertoire of visual opsins, transducin alpha subunits and oxytocin/vasopressin receptors was established by duplication of their shared genomic region in the two rounds of early vertebrate genome duplications.
Lagman D, Ocampo Daza D, Widmark J, Abalo XM, Sundstr?m G, Larhammar D
BMC evolutionary biology13238(2013 Nov)

Mutually exclusive expression of G?ia and G?14 reveals diversification of taste receptor cells in zebrafish.
Ohmoto M, Okada S, Nakamura S, Abe K, Matsumoto I
The Journal of comparative neurology519(8)1616-29(2011 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following gnai3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gnai3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa35163	NM_001111250.1 Latest version!	Danio rerio guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 3 (gnai3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

gnai3 ( NM_001111250.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001104720.1 Danio rerio guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 3 (gnai3), mRNA.

Price & Availability↑

CloneID	ODa35163
Clone ID Related Accession (Same CDS sequence)	NM_001111250.1
Accession Version	NM_001111250.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1065bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	guanine nucleotide-binding protein G(k) subunit alpha
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC155288.1. On Dec 9, 2007 this sequence version replaced XM_001343090.1. ##Evidence-Data-START## Transcript exon combination :: BC155288.1, BC171642.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGGTTGCA CGTTAAGCAC TGAAGATAAA GCCGCGATGG AGAAGAGTAA GATGATTGAC 
CGGACCCTGC GAGAGGACGG AGAGAAAGCA TCCAGAGAGG TCAAACTCCT GCTGCTCGGT 
GCTGGGGAAT CAGGGAAGAG TACTATCGTT AAGCAGATGA AGATCATTCA TGAAGATGGT 
TACTCAGAAG ACGAGTGCAA GCAGTATAAA GTGGTGGTTT ACAGCAACAC TATCCAGTCC 
ATCATTGCCA TCATCAGGGC CATGGGACGA TTAAAAATCG ACTTTGGGGA TCCGGCAAGA 
GCGGATGATG CCCGTCAGCT GTTTGTGCTC GCTGGCACTG CTGAGGAAGG GGTGATGACG 
GCTGATCTCT CCGGGGTCAT CCGCCGGCTG TGGAAGGATG AAGGAGTACA GATGTGTTTC 
GTCAGGTCAC GAGAGTATCA ACTCAACGAT TCTGCAGCCT ACTATTTGAA TGATCTGGAA 
AGGATATCAC AGCCCACCTA CACACCCACT CAACAAGATG TGCTGAGGAC TCGGGTCAAG 
ACCACTGGCA TTGTAGAAAC ACACTTCACC TTCAAAGAGC TCTATTTCAA GATGTTTGAT 
GTGGGTGGCC AGCGTTCTGA GCGGAAGAAG TGGATTCATT GTTTTGAAGG AGTCACTGCC 
ATCATCTTCT GTGTGGCGCT CAGCGACTAT GACCTGGTGC TTGCTGAGGA TGAAGAGATG 
AACCGCATGC ATGAGAGCAT GAAGCTTTTT GACAGCATCT GCAACAACAA GTGGTTCACA 
GACACGTCCG TCATCCTCTT CCTCAATAAG AAGGATCTGT TCGAGGACAA GATCAAGAAA 
AGTCCACTTA CAATATGCTA CCCTGAATAC TGCGGGACAT GCACGTACGA GGAAGCAGCG 
GCGTACATTC AGTGCCAGTT TGAGGATCTG AACAAAAGAA AGGAGACAAA GGAGATCTAC 
ACACACTTCA CCTGTGCCAC AGACACCAAG AATGTACAGT TTGTGTTTGA TGCAGTCACT 
GATGTCATCA TCAAGAATAA CCTCATTGAG TGTGGGCTCT ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001104720.1
CDS	459..1523
Translation	MGCTLSTEDKAAMEKSKMIDRTLREDGEKASREVKLLLLGAGESGKSTIVKQMKIIHEDGYSEDECKQYKVVVYSNTIQSIIAIIRAMGRLKIDFGDPARADDARQLFVLAGTAEEGVMTADLSGVIRRLWKDEGVQMCFVRSREYQLNDSAAYYLNDLERISQPTYTPTQQDVLRTRVKTTGIVETHFTFKELYFKMFDVGGQRSERKKWIHCFEGVTAIIFCVALSDYDLVLAEDEEMNRMHESMKLFDSICNNKWFTDTSVILFLNKKDLFEDKIKKSPLTICYPEYCGTCTYEEAAAYIQCQFEDLNKRKETKEIYTHFTCATDTKNVQFVFDAVTDVIIKNNLIECGLY

Target ORF information:

RefSeq Version	NM_001111250.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 3 (gnai3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001111250.1

ORF Insert Sequence:

ATGGGTTGCA CGTTAAGCAC TGAAGATAAA GCCGCGATGG AGAAGAGTAA GATGATTGAC 
CGGACCCTGC GAGAGGACGG AGAGAAAGCA TCCAGAGAGG TCAAACTCCT GCTGCTCGGT 
GCTGGGGAAT CAGGGAAGAG TACTATCGTT AAGCAGATGA AGATCATTCA TGAAGATGGT 
TACTCAGAAG ACGAGTGCAA GCAGTATAAA GTGGTGGTTT ACAGCAACAC TATCCAGTCC 
ATCATTGCCA TCATCAGGGC CATGGGACGA TTAAAAATCG ACTTTGGGGA TCCGGCAAGA 
GCGGATGATG CCCGTCAGCT GTTTGTGCTC GCTGGCACTG CTGAGGAAGG GGTGATGACG 
GCTGATCTCT CCGGGGTCAT CCGCCGGCTG TGGAAGGATG AAGGAGTACA GATGTGTTTC 
GTCAGGTCAC GAGAGTATCA ACTCAACGAT TCTGCAGCCT ACTATTTGAA TGATCTGGAA 
AGGATATCAC AGCCCACCTA CACACCCACT CAACAAGATG TGCTGAGGAC TCGGGTCAAG 
ACCACTGGCA TTGTAGAAAC ACACTTCACC TTCAAAGAGC TCTATTTCAA GATGTTTGAT 
GTGGGTGGCC AGCGTTCTGA GCGGAAGAAG TGGATTCATT GTTTTGAAGG AGTCACTGCC 
ATCATCTTCT GTGTGGCGCT CAGCGACTAT GACCTGGTGC TTGCTGAGGA TGAAGAGATG 
AACCGCATGC ATGAGAGCAT GAAGCTTTTT GACAGCATCT GCAACAACAA GTGGTTCACA 
GACACGTCCG TCATCCTCTT CCTCAATAAG AAGGATCTGT TCGAGGACAA GATCAAGAAA 
AGTCCACTTA CAATATGCTA CCCTGAATAC TGCGGGACAT GCACGTACGA GGAAGCAGCG 
GCGTACATTC AGTGCCAGTT TGAGGATCTG AACAAAAGAA AGGAGACAAA GGAGATCTAC 
ACACACTTCA CCTGTGCCAC AGACACCAAG AATGTACAGT TTGTGTTTGA TGCAGTCACT 
GATGTCATCA TCAAGAATAA CCTCATTGAG TGTGGGCTCT ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Distinct functions of two olfactory marker protein genes derived from teleost-specific whole genome duplication.
BMC evolutionary biology15245(2015 Nov)
Suzuki H,Nikaido M,Hagino-Yamagishi K,Okada N

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

The vertebrate ancestral repertoire of visual opsins, transducin alpha subunits and oxytocin/vasopressin receptors was established by duplication of their shared genomic region in the two rounds of early vertebrate genome duplications.
BMC evolutionary biology13238(2013 Nov)
Lagman D,Ocampo Daza D,Widmark J,Abalo XM,Sundstr?m G,Larhammar D

Mutually exclusive expression of G?ia and G?14 reveals diversification of taste receptor cells in zebrafish.
The Journal of comparative neurology519(8)1616-29(2011 Jun)
Ohmoto M,Okada S,Nakamura S,Abe K,Matsumoto I