GNAI3 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	GNAI3
Entrez Gene ID	2773
Full Name	G protein subunit alpha i3
Synonyms	87U6,ARCND1
General protein information	Preferred Names G protein subunit alpha i3 Names guanine nucleotide-binding protein G(k) subunit alpha g(i) alpha-3 guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 3
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1p13.3
Summary	Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling pathways. G proteins are composed of 3 units: alpha, beta and gamma. This gene encodes an alpha subunit and belongs to the G-alpha family. Mutation in this gene, resulting in a gly40-to-arg substitution, is associated with auriculocondylar syndrome, and shown to affect downstream targets in the G protein-coupled endothelin receptor pathway. [provided by RefSeq, Jun 2012].
Disorder MIM:	139370
Disorder Html:	Auriculocondylar syndrome 1, 602483 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_006496.3	NP_006487.1	guanine nucleotide-binding protein G(k) subunit alpha
NM_006496.4	NP_006487.1	guanine nucleotide-binding protein G(i) subunit alpha

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	GNAI3	XP_513624.1
	GNAI3	XP_001091470.1
Bos taurus (cattle)	GNAI3	NP_001029849.1
Rattus norvegicus (Norway rat)	Gnai3	NP_037238.1
Canis lupus familiaris (dog)	GNAI3	XP_854614.3
Xenopus tropicalis (tropical clawed frog)	gnai3	NP_001011471.1
Homo sapiens (human)	GNAI3	NP_006487.1
Danio rerio (zebrafish)	gnai3	NP_001104720.1
Mus musculus (house mouse)	Gnai3	NP_034436.1
Gallus gallus (chicken)	GNAI3	NP_989580.1

Gene Ontology
Bibliography

Related articles in PubMed

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

G?i3 nuclear translocation causes irradiation resistance in human glioma cells.
Cai S, Li Y, Bai JY, Zhang ZQ, Wang Y, Qiao YB, Zhou XZ, Yang B, Tian Y, Cao C
Oncotarget8(21)35061-35068(2017 May)

Role of GATA binding protein 4 (GATA4) in the regulation of tooth development via GNAI3.
Guo S, Zhang Y, Zhou T, Wang D, Weng Y, Wang L, Ma J
Scientific reports7(1)1534(2017 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These data indicate that, unlike in taste cells, TAS2Rs couple to the prevalent G proteins, Galphai1, Galphai2, and Galphai3, with no evidence for functional coupling to Galphagust.
Title: Coupling of Airway Smooth Muscle Bitter Taste Receptors to Intracellular Signaling and Relaxation Is via G

Data show that auriculo-condylar syndrome (ACS)-associated mutations in G protein subunit alpha i3 (GNAI3) produce dominant-negative Galpha(i3) mutant proteins that couple to endothelin type A receptor (ET(A)R).
Title: Dominant-negative G? subunits are a mechanism of dysregulated heterotrimeric G protein signaling in human disease.

GNAI3 is identified a second gene possibly responsible for X-linked ocular albinism.
Title: GNAI3: Another Candidate Gene to Screen in Persons with Ocular Albinism.

In postmortem human prefrontal cortex, adenosine A1 receptor is coupled preferentially, if not exclusively, to Galphai-3.
Title: Adenosine A1( )receptors are selectively coupled to G?(i-3) in postmortem human brain cortex: Guanosine-5'-O-(3-[(35)S]thio)triphosphate ([(35)S]GTP?S) binding/immunoprecipitation study.

GIV and its substrate Galphai3 are recruited to active integrin complexes
Title: Integrins activate trimeric G proteins via the nonreceptor protein GIV/Girdin.

The following GNAI3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GNAI3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu19778	NM_006496.4 Latest version!	Homo sapiens G protein subunit alpha i3 (GNAI3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
View Old Accession Versions >>
OHu19778	NM_006496.3	Homo sapiens G protein subunit alpha i3 (GNAI3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GNAI3 ( NM_006496.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_006487.1 Homo sapiens G protein subunit alpha i3 (GNAI3), mRNA.

Price & Availability↑

CloneID	OHu19778
Clone ID Related Accession (Same CDS sequence)	NM_006496.3 , NM_006496.4
Accession Version	NM_006496.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1065bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	guanine nucleotide-binding protein G(k) subunit alpha
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP289420.1, BC025285.1, BE886720.1, BU195094.1, AW984247.1, CX165017.1, BI492787.1, AI684171.1 and BU622030.1. This sequence is a reference standard in the RefSeqGene project. On Jun 14, 2012 this sequence version replaced NM_006496.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.268128.1, SRR1660807.112929.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCTGCA CGTTGAGCGC CGAAGACAAG GCGGCAGTGG AGCGAAGCAA GATGATCGAC 
CGCAACTTAC GGGAGGACGG GGAAAAAGCG GCCAAAGAAG TGAAGCTGCT GCTACTCGGT 
GCTGGAGAAT CTGGTAAAAG CACCATTGTG AAACAGATGA AAATCATTCA TGAGGATGGC 
TATTCAGAGG ATGAATGTAA ACAATATAAA GTAGTTGTCT ACAGCAATAC TATACAGTCC 
ATCATTGCAA TCATAAGAGC CATGGGACGG CTAAAGATTG ACTTTGGGGA AGCTGCCAGG 
GCAGATGATG CCCGGCAATT ATTTGTTTTA GCTGGCAGTG CTGAAGAAGG AGTCATGACT 
CCAGAACTAG CAGGAGTGAT TAAACGGTTA TGGCGAGATG GTGGGGTACA AGCTTGCTTC 
AGCAGATCCA GGGAATATCA GCTCAATGAT TCTGCTTCAT ATTATCTAAA TGATCTGGAT 
AGAATATCCC AGTCTAACTA CATTCCAACT CAGCAAGATG TTCTTCGGAC GAGAGTGAAG 
ACCACAGGCA TTGTAGAAAC ACATTTCACC TTCAAAGACC TATACTTCAA GATGTTTGAT 
GTAGGTGGCC AAAGATCAGA ACGAAAAAAG TGGATTCACT GTTTTGAGGG AGTGACAGCA 
ATTATCTTCT GTGTGGCCCT CAGTGATTAT GACCTTGTTC TGGCTGAGGA CGAGGAGATG 
AACCGAATGC ATGAAAGCAT GAAACTGTTT GACAGCATTT GTAATAACAA ATGGTTTACA 
GAAACTTCAA TCATTCTCTT CCTTAACAAG AAAGACCTTT TTGAGGAAAA AATAAAGAGG 
AGTCCGTTAA CTATCTGTTA TCCAGAATAC ACAGGTTCCA ATACATATGA AGAGGCAGCT 
GCCTATATTC AATGCCAGTT TGAAGATCTG AACAGAAGAA AAGATACCAA GGAGATCTAT 
ACTCACTTCA CCTGTGCCAC AGACACGAAG AATGTGCAGT TTGTTTTTGA TGCTGTTACA 
GATGTCATCA TTAAAAACAA CTTAAAGGAA TGTGGACTTT ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006487.1
CDS	158..1222
Translation	MGCTLSAEDKAAVERSKMIDRNLREDGEKAAKEVKLLLLGAGESGKSTIVKQMKIIHEDGYSEDECKQYKVVVYSNTIQSIIAIIRAMGRLKIDFGEAARADDARQLFVLAGSAEEGVMTPELAGVIKRLWRDGGVQACFSRSREYQLNDSASYYLNDLDRISQSNYIPTQQDVLRTRVKTTGIVETHFTFKDLYFKMFDVGGQRSERKKWIHCFEGVTAIIFCVALSDYDLVLAEDEEMNRMHESMKLFDSICNNKWFTETSIILFLNKKDLFEEKIKRSPLTICYPEYTGSNTYEEAAAYIQCQFEDLNRRKDTKEIYTHFTCATDTKNVQFVFDAVTDVIIKNNLKECGLY

Target ORF information:

RefSeq Version	NM_006496.3
Organism	Homo sapiens(human)
Definition	Homo sapiens G protein subunit alpha i3 (GNAI3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006496.3

ORF Insert Sequence:

ATGGGCTGCA CGTTGAGCGC CGAAGACAAG GCGGCAGTGG AGCGAAGCAA GATGATCGAC 
CGCAACTTAC GGGAGGACGG GGAAAAAGCG GCCAAAGAAG TGAAGCTGCT GCTACTCGGT 
GCTGGAGAAT CTGGTAAAAG CACCATTGTG AAACAGATGA AAATCATTCA TGAGGATGGC 
TATTCAGAGG ATGAATGTAA ACAATATAAA GTAGTTGTCT ACAGCAATAC TATACAGTCC 
ATCATTGCAA TCATAAGAGC CATGGGACGG CTAAAGATTG ACTTTGGGGA AGCTGCCAGG 
GCAGATGATG CCCGGCAATT ATTTGTTTTA GCTGGCAGTG CTGAAGAAGG AGTCATGACT 
CCAGAACTAG CAGGAGTGAT TAAACGGTTA TGGCGAGATG GTGGGGTACA AGCTTGCTTC 
AGCAGATCCA GGGAATATCA GCTCAATGAT TCTGCTTCAT ATTATCTAAA TGATCTGGAT 
AGAATATCCC AGTCTAACTA CATTCCAACT CAGCAAGATG TTCTTCGGAC GAGAGTGAAG 
ACCACAGGCA TTGTAGAAAC ACATTTCACC TTCAAAGACC TATACTTCAA GATGTTTGAT 
GTAGGTGGCC AAAGATCAGA ACGAAAAAAG TGGATTCACT GTTTTGAGGG AGTGACAGCA 
ATTATCTTCT GTGTGGCCCT CAGTGATTAT GACCTTGTTC TGGCTGAGGA CGAGGAGATG 
AACCGAATGC ATGAAAGCAT GAAACTGTTT GACAGCATTT GTAATAACAA ATGGTTTACA 
GAAACTTCAA TCATTCTCTT CCTTAACAAG AAAGACCTTT TTGAGGAAAA AATAAAGAGG 
AGTCCGTTAA CTATCTGTTA TCCAGAATAC ACAGGTTCCA ATACATATGA AGAGGCAGCT 
GCCTATATTC AATGCCAGTT TGAAGATCTG AACAGAAGAA AAGATACCAA GGAGATCTAT 
ACTCACTTCA CCTGTGCCAC AGACACGAAG AATGTGCAGT TTGTTTTTGA TGCTGTTACA 
GATGTCATCA TTAAAAACAA CTTAAAGGAA TGTGGACTTT ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GNAI3 ( NM_006496.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_006487.1 Homo sapiens G protein subunit alpha i3 (GNAI3), mRNA.

Price & Availability↑

CloneID	OHu19778
Clone ID Related Accession (Same CDS sequence)	NM_006496.3 , NM_006496.4
Accession Version	NM_006496.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1065bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	guanine nucleotide-binding protein G(i) subunit alpha
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL355310.20. On May 24, 2019 this sequence version replaced NM_006496.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.156507.1, SRR1803617.43796.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGCTGCA CGTTGAGCGC CGAAGACAAG GCGGCAGTGG AGCGAAGCAA GATGATCGAC 
CGCAACTTAC GGGAGGACGG GGAAAAAGCG GCCAAAGAAG TGAAGCTGCT GCTACTCGGT 
GCTGGAGAAT CTGGTAAAAG CACCATTGTG AAACAGATGA AAATCATTCA TGAGGATGGC 
TATTCAGAGG ATGAATGTAA ACAATATAAA GTAGTTGTCT ACAGCAATAC TATACAGTCC 
ATCATTGCAA TCATAAGAGC CATGGGACGG CTAAAGATTG ACTTTGGGGA AGCTGCCAGG 
GCAGATGATG CCCGGCAATT ATTTGTTTTA GCTGGCAGTG CTGAAGAAGG AGTCATGACT 
CCAGAACTAG CAGGAGTGAT TAAACGGTTA TGGCGAGATG GTGGGGTACA AGCTTGCTTC 
AGCAGATCCA GGGAATATCA GCTCAATGAT TCTGCTTCAT ATTATCTAAA TGATCTGGAT 
AGAATATCCC AGTCTAACTA CATTCCAACT CAGCAAGATG TTCTTCGGAC GAGAGTGAAG 
ACCACAGGCA TTGTAGAAAC ACATTTCACC TTCAAAGACC TATACTTCAA GATGTTTGAT 
GTAGGTGGCC AAAGATCAGA ACGAAAAAAG TGGATTCACT GTTTTGAGGG AGTGACAGCA 
ATTATCTTCT GTGTGGCCCT CAGTGATTAT GACCTTGTTC TGGCTGAGGA CGAGGAGATG 
AACCGAATGC ATGAAAGCAT GAAACTGTTT GACAGCATTT GTAATAACAA ATGGTTTACA 
GAAACTTCAA TCATTCTCTT CCTTAACAAG AAAGACCTTT TTGAGGAAAA AATAAAGAGG 
AGTCCGTTAA CTATCTGTTA TCCAGAATAC ACAGGTTCCA ATACATATGA AGAGGCAGCT 
GCCTATATTC AATGCCAGTT TGAAGATCTG AACAGAAGAA AAGATACCAA GGAGATCTAT 
ACTCACTTCA CCTGTGCCAC AGACACGAAG AATGTGCAGT TTGTTTTTGA TGCTGTTACA 
GATGTCATCA TTAAAAACAA CTTAAAGGAA TGTGGACTTT ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006487.1
CDS	107..1171
Translation	MGCTLSAEDKAAVERSKMIDRNLREDGEKAAKEVKLLLLGAGESGKSTIVKQMKIIHEDGYSEDECKQYKVVVYSNTIQSIIAIIRAMGRLKIDFGEAARADDARQLFVLAGSAEEGVMTPELAGVIKRLWRDGGVQACFSRSREYQLNDSASYYLNDLDRISQSNYIPTQQDVLRTRVKTTGIVETHFTFKDLYFKMFDVGGQRSERKKWIHCFEGVTAIIFCVALSDYDLVLAEDEEMNRMHESMKLFDSICNNKWFTETSIILFLNKKDLFEEKIKRSPLTICYPEYTGSNTYEEAAAYIQCQFEDLNRRKDTKEIYTHFTCATDTKNVQFVFDAVTDVIIKNNLKECGLY

Target ORF information:

RefSeq Version	NM_006496.4
Organism	Homo sapiens(human)
Definition	Homo sapiens G protein subunit alpha i3 (GNAI3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006496.4

ORF Insert Sequence:

ATGGGCTGCA CGTTGAGCGC CGAAGACAAG GCGGCAGTGG AGCGAAGCAA GATGATCGAC 
CGCAACTTAC GGGAGGACGG GGAAAAAGCG GCCAAAGAAG TGAAGCTGCT GCTACTCGGT 
GCTGGAGAAT CTGGTAAAAG CACCATTGTG AAACAGATGA AAATCATTCA TGAGGATGGC 
TATTCAGAGG ATGAATGTAA ACAATATAAA GTAGTTGTCT ACAGCAATAC TATACAGTCC 
ATCATTGCAA TCATAAGAGC CATGGGACGG CTAAAGATTG ACTTTGGGGA AGCTGCCAGG 
GCAGATGATG CCCGGCAATT ATTTGTTTTA GCTGGCAGTG CTGAAGAAGG AGTCATGACT 
CCAGAACTAG CAGGAGTGAT TAAACGGTTA TGGCGAGATG GTGGGGTACA AGCTTGCTTC 
AGCAGATCCA GGGAATATCA GCTCAATGAT TCTGCTTCAT ATTATCTAAA TGATCTGGAT 
AGAATATCCC AGTCTAACTA CATTCCAACT CAGCAAGATG TTCTTCGGAC GAGAGTGAAG 
ACCACAGGCA TTGTAGAAAC ACATTTCACC TTCAAAGACC TATACTTCAA GATGTTTGAT 
GTAGGTGGCC AAAGATCAGA ACGAAAAAAG TGGATTCACT GTTTTGAGGG AGTGACAGCA 
ATTATCTTCT GTGTGGCCCT CAGTGATTAT GACCTTGTTC TGGCTGAGGA CGAGGAGATG 
AACCGAATGC ATGAAAGCAT GAAACTGTTT GACAGCATTT GTAATAACAA ATGGTTTACA 
GAAACTTCAA TCATTCTCTT CCTTAACAAG AAAGACCTTT TTGAGGAAAA AATAAAGAGG 
AGTCCGTTAA CTATCTGTTA TCCAGAATAC ACAGGTTCCA ATACATATGA AGAGGCAGCT 
GCCTATATTC AATGCCAGTT TGAAGATCTG AACAGAAGAA AAGATACCAA GGAGATCTAT 
ACTCACTTCA CCTGTGCCAC AGACACGAAG AATGTGCAGT TTGTTTTTGA TGCTGTTACA 
GATGTCATCA TTAAAAACAA CTTAAAGGAA TGTGGACTTT ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

G?i3 nuclear translocation causes irradiation resistance in human glioma cells.
Oncotarget8(21)35061-35068(2017 May)
Cai S,Li Y,Bai JY,Zhang ZQ,Wang Y,Qiao YB,Zhou XZ,Yang B,Tian Y,Cao C

Role of GATA binding protein 4 (GATA4) in the regulation of tooth development via GNAI3.
Scientific reports7(1)1534(2017 May)
Guo S,Zhang Y,Zhou T,Wang D,Weng Y,Wang L,Ma J

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GNAI3 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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