nphp-4 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	nphp-4
Entrez Gene ID	179620
Full Name	NePHronoPhthisis (human kidney disease) homolog
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_073581.3	NP_505982.2	NePHronoPhthisis (human kidney disease) homolog
NM_073581.2	NP_505982.2	NePHronoPhthisis (human kidney disease) homolog

Pathways from BioSystems

Homologies

Bos taurus (cattle)	NPHP4	XP_005217234.1
Caenorhabditis elegans (roundworm)	nphp-4	NP_505982.2
Pan troglodytes (chimpanzee)	NPHP4	XP_003307840.2
	NPHP4	XP_002802192.1
Homo sapiens (human)	NPHP4	NP_055917.1
Canis lupus familiaris (dog)	NPHP4	NP_001129260.1
Mus musculus (house mouse)	Nphp4	NP_700473.2
Danio rerio (zebrafish)	nphp4	XP_005167009.1
Rattus norvegicus (Norway rat)	Nphp4	NP_001032739.2
Gallus gallus (chicken)	NPHP4	XP_003642568.2

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

OSM-3 mutations cause NPHP-4 dependent and independent phenotypes.
Title: A Screen for Modifiers of Cilia Phenotypes Reveals Novel MKS Alleles and Uncovers a Specific Genetic Interaction between osm-3 and nphp-4.

data indicate a genetic interaction between mutations in mks-3 and nphp-4 causes cilia and sensilla morphology defects
Title: Normal ciliogenesis requires synergy between the cystic kidney disease genes MKS-3 and NPHP-4.

The C. elegans B9 proteins form a complex that localizes to the base of cilia. Mutations in the B9 genes do not overtly affect cilia formation unless they are in combination with a mutation in nph-1 or nph-4.
Title: Functional redundancy of the B9 proteins and nephrocystins in Caenorhabditis elegans ciliogenesis.

We propose that NPHP-1 and NPHP-4 act globally at the TZ to regulate ciliary access of the IFT machinery, axonemal structural components, and signaling molecules, and that perturbing this balance results in cell type-specific phenotypes.
Title: The Caenorhabditis elegans nephrocystins act as global modifiers of cilium structure.

NPHP-4 protein plays important and redundant role in facilitating ciliary sensory signal transduction.
Title: Functional characterization of the C. elegans nephrocystins NPHP-1 and NPHP-4 and their role in cilia and male sensory behaviors.

The following nphp-4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nphp-4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa345044	NM_073581.3 Latest version!	Caenorhabditis elegans NePHronoPhthisis (human kidney disease) homolog (nphp-4), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
OCa345044	NM_073581.2	Caenorhabditis elegans NePHronoPhthisis (human kidney disease) homolog (nphp-4), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nphp-4 ( NM_073581.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_505982.2 Caenorhabditis elegans NePHronoPhthisis (human kidney disease) homolog (nphp-4), partial mRNA.

Price & Availability↑

CloneID	OCa345044
Clone ID Related Accession (Same CDS sequence)	NM_073581.3 , NM_073581.2
Accession Version	NM_073581.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3918bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	NePHronoPhthisis (human kidney disease) homolog
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). On Sep 4, 2019 this sequence version replaced NM_073581.2. COMPLETENESS: incomplete on the 5' end.

ATGTCGGTCA ACGACTGGTA TTCGTTGTTT CTGGCAAACC GGCCAGTTGA AATGAAACGT 
AATGTTTCGA GAGGAACTAA AGCTTTGTGT TATTCAATGT TCATTTCCAA TTTGACATCT 
CCACAGCTGA CTGAGAACAT TCGATACCAG ATTAGTGCTT TTTTATTCGA CACGAAGACT 
TCTCAAATGT TTGGGAGGCA ATGTAGAACA GAATGGATTC CAGCGAATAG TAATGGAACT 
TGTGTTTTTA ATGAAACATT ATATTTTTAC TCAATTATAA ACAGCAGAGA TGTGCTTCTT 
ATTCTTGAAT TTGTGGAAGA AGGATCAGAC GAAATAACGC CTGCCACAAG CGTTGGATGG 
TTTTCAACGC ATATCGAGAA GAAAACGCCC GTAGAAATAT CAAACACTAA AATTTTCGAT 
ATATTTGGTG GAACTCCAAA ACTATTAATT TTTGATAAAG AAACAGTTTT AAAACCAGTT 
GGAAATGTTG AATGTACATA CAACATATTC GAAATGCCAC CCATATTCTT CCAATGTTTG 
CCTGAATTTT GCATTGTTTG CGACAAAGAC ATTATTCCTG GAATAATCAA AGACAGTTCT 
GATGAATGGT GGTTGAGCAC GCCAAAAGAG ATGCCAACAA TTCCCGCTGC AATTGATGCA 
ATTGTAATTC AATTCAAGAA TAATGTACCT GAATTAGAAA AACAAATTAC ACATGATATT 
GAAAAAGAAT GGGCATTGAA AGAAGGAGGA ACTCTTAAAC CAAAAGCAAT CATAATGGAT 
AGAAAATTGA GAATCGGAGT ACACAATGGG TACACATATG TCACTGAACC ATTCACAGTT 
GACTTGGAAA TAATCTCCTC AAATGCTGGT GATACTCTAA GATCGCGAAA GAAACCTATT 
GACTTTGGGA AATCATCAAA TTGGGAAGAA CAACTGCTAT TTCAAGCTGC TGGAAACCCG 
CGTCTGGCAC TTCGAAATCT GTATGCGGAT CCAAGAATGG CTATCATATT CTTGCTTGAA 
TACACCTTTC ACCGAGAAGA CAATCAAAGC CTTAATCAGA CTATTCTAAT CGGATGGGCT 
GCATGGACTC CATTCTCAGA TGGAGCCTTT TCTGGAAAAG AAGTGGAGAC ACGAGTTAGC 
TTTGTTGGTG GTCCACGTCC GAATCCAGAA GGCGTTTTAT GTTATAAGAA CGTATTGAAT 
CAACCAGACT CCTTGAAGCC ATTGAATGAG AAACTTGAAA TTTTTGTGGA CTTTAAATTT 
TATGAAAATG GAAGAAGTGT TCACAATACA CCAACATCAA GAAGGGCTGC TGACTCTGCA 
AGAGTTCAAA CTGGAAGATC AGGCGATAAT GGCCAATCTG CTAGATCAAA TAGAAAATCA 
GTGAAAATAG AAACTCCTAG AAGTCCAGAA AATTCGAATA GATTCCCTGC ACTAGTAGAT 
ACAGGAAGAT CAGTTTCATC AGTAGATGAA TTGAGATCAA TAAATGAGGA TTTGAATAGA 
TTCATAGAAG AACCAATGGA AATTCCAGTT CAAGACGTAG TTGTTGCAAA GAAACCAGTA 
GAAGAACCTC TACCAATAAC ATCAGTTTAC AAGATTCCTT TTGATGAGCT CAAACCAATA 
AACTTCCCAA GATCTGCCCA TTCCATGTTT GCGAGACAAA ACTTCACTCA GTTGAAAGAC 
AGGAACGGAT CACCACCAAA TACTGAAGAT GTGACACTAA AAACCATCAT CGATATGAAA 
AGAGAGCAAT TGGACAGACT CATTACATCA CATGTATATT TTCAATTTAT TGCTTTCAAA 
CAACTTGCTG CTCCTGATGC TCGCATGATT AAGAAGTTAT TTTTCACAAT CGGGTTTTAC 
CGATTCCCCG ATATTACAAC AGAGTCAATG CTTCTCACAT CTATGGAGAA AGGAGAACCA 
ACCCTTTTAA CCCGACTTGA TAAAAATGGG AATTCGGATG TTATAGCTTC ACCTGGATTC 
ATAGCAAAAT ATATAATCGA AGGAGAAGAA AGCAAGGCTG ACTTTTTGGA TTTTATGGCT 
TCTGGTCATG CAACTATTGA TGTATGGGAT TCAGACTCGT TGATTCACTT GGGATCAACT 
ATTGTGCCAA TCAAGAATTT GTATCGAAGA GGAAGGGAAG CTGTTCAGTT GTTTATTCAA 
TGCCCAGTGG TTGATACATC TTTAGACACT TCATCAAAAG CTGGGGCTTT CCTTTACATG 
AGAGTTGCCA ATATCGGATT TCCGTCAGGC AATACATATG ATCTCTCCTC CTCCTCTTCA 
TCGCTGACTA CCACAAGATC CAACGTAAAC TCTGGACAAG GTACAGTAGT CCGCCGACTG 
ACGTCATCAA TCCGGTTAAA TGAAGAAGGA CCCCATTCCT ATCGAATTCA CGCAAAACCA 
TTACCTGGTA ATAGTGGTGT TGGACTAGAT CGGTTTTTGA CAGCTCAGAG ACTAGATATT 
CAACAACGGC ATGAACAATT GTTCAATGAG AATAGTTTGG ATAAGATCAG ACAATGGAAT 
GATTTAAAAG AAGGATTCAA CTTTTCTGAT AATAAAGAAA TTGCACAGAA ATTCATTTTT 
GAAGAAGAGC TAGCTGCATA CAAAAAGCTT CGATATGAAA GTAAACCTGC AAAACTTCTT 
GAAGCTGTTT TCAAAGGTAT TACAAGTTGT CACCAAATAA ATCCCAGTTT TGGAGAGAAA 
GTATTTTTTG AATTTCCTCT CGAGAACTAC AATTCTGAAC CAATCAATTG CACCATTGAA 
TTTGATGATG AAGCTTTGAA ACCAGTATTT GATGCAGAAG AATGGAAATT TTACAAAACA 
GTGAACAAAG TAACAACTCC ATCAGAAAAG CAAATGATGA GACAAACAAC GGATAGAATT 
GAGATTTGTC TTCAACCTGG GGATGTTTTA TTTATTCCAT TTATCTACGA CGCTTTCTTT 
TTCCCAAATG ATGCATTCAA CATGTACTCA ACAAAAGTTG TATTTAGAAG ATGGGATACA 
AAAGAACCAC TAGCAATATT GGATCTCCAC GTGCATCGAA GAAATTTCCT TCTTCAACAT 
TCAGTTACTT TTATTTGTGA AACATCTGGA AACTGGGAAA AACAATTAGT ACTACCACCA 
ATGGCCAGAG ACAGAAGAGT TTTGTCATGT CGATGCTCTG ATCCGTCTGT AAGACTGACT 
GTTCGAAATG CGACACTACA ACAGATTGTT GGATTCACAA CATATTCAGG GGAAACGAAT 
GATCGAAAGA CGTTTCTTTT ATTGATGTAT AGTGATCATT ATCAAACAAG ATTAATGGCT 
ACCTGGAAAA TCACTATTCT TCCATTCTTC AATGTTGATG TTCGTAGTAT TGTTGGGCAA 
ACAACTCGTC TTCATTTATT AGTGCACAGA AGAAGCGAGC ATGATGGAGT GCCAGATGAT 
TTACTGAAAG TGTATACAGC ATCTGGATGT ATGAAAGTTG TGGATTCTGT GCTCACTGAA 
AGAACACCTA CAGCCACAAT TGATTTTACA CCAAACTTTA TTGGAACAAA GAAGCTTGTC 
GTTTCTGTTG TGAATACCAA TACATTGAAG TTAGAACGAG GGTTTTTGGT TTACGGAAAG 
TCTGAAGCGC CACGGATTAC GCAGAAGTTT GTCATACAAA TTCCATCATC TGATGAAGCT 
ATTAGAAAGA GAATCCCGAT AAGAAACCCA TATGGTTTAC CAAAGACATT CCGAATCACC 
ACATCAAATT CTGATATTGT AAAAATTACG GATAGTCTGT TGTCAGTTCC ACCTATGGGA 
AAACTTCCAT GTGAAATGTA TTTTGTGAAG AATACACATC TTCAAAAGAA TATAGAGACA 
TTGTTGTATA TATCAGATGC GGAGACTTAT GTACAAGAAG AAGCATATTC AATCACACTT 
GCATTCGAAG CTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_505982.2
CDS	1..3918
Translation	MSVNDWYSLFLANRPVEMKRNVSRGTKALCYSMFISNLTSPQLTENIRYQISAFLFDTKTSQMFGRQCRTEWIPANSNGTCVFNETLYFYSIINSRDVLLILEFVEEGSDEITPATSVGWFSTHIEKKTPVEISNTKIFDIFGGTPKLLIFDKETVLKPVGNVECTYNIFEMPPIFFQCLPEFCIVCDKDIIPGIIKDSSDEWWLSTPKEMPTIPAAIDAIVIQFKNNVPELEKQITHDIEKEWALKEGGTLKPKAIIMDRKLRIGVHNGYTYVTEPFTVDLEIISSNAGDTLRSRKKPIDFGKSSNWEEQLLFQAAGNPRLALRNLYADPRMAIIFLLEYTFHREDNQSLNQTILIGWAAWTPFSDGAFSGKEVETRVSFVGGPRPNPEGVLCYKNVLNQPDSLKPLNEKLEIFVDFKFYENGRSVHNTPTSRRAADSARVQTGRSGDNGQSARSNRKSVKIETPRSPENSNRFPALVDTGRSVSSVDELRSINEDLNRFIEEPMEIPVQDVVVAKKPVEEPLPITSVYKIPFDELKPINFPRSAHSMFARQNFTQLKDRNGSPPNTEDVTLKTIIDMKREQLDRLITSHVYFQFIAFKQLAAPDARMIKKLFFTIGFYRFPDITTESMLLTSMEKGEPTLLTRLDKNGNSDVIASPGFIAKYIIEGEESKADFLDFMASGHATIDVWDSDSLIHLGSTIVPIKNLYRRGREAVQLFIQCPVVDTSLDTSSKAGAFLYMRVANIGFPSGNTYDLSSSSSSLTTTRSNVNSGQGTVVRRLTSSIRLNEEGPHSYRIHAKPLPGNSGVGLDRFLTAQRLDIQQRHEQLFNENSLDKIRQWNDLKEGFNFSDNKEIAQKFIFEEELAAYKKLRYESKPAKLLEAVFKGITSCHQINPSFGEKVFFEFPLENYNSEPINCTIEFDDEALKPVFDAEEWKFYKTVNKVTTPSEKQMMRQTTDRIEICLQPGDVLFIPFIYDAFFFPNDAFNMYSTKVVFRRWDTKEPLAILDLHVHRRNFLLQHSVTFICETSGNWEKQLVLPPMARDRRVLSCRCSDPSVRLTVRNATLQQIVGFTTYSGETNDRKTFLLLMYSDHYQTRLMATWKITILPFFNVDVRSIVGQTTRLHLLVHRRSEHDGVPDDLLKVYTASGCMKVVDSVLTERTPTATIDFTPNFIGTKKLVVSVVNTNTLKLERGFLVYGKSEAPRITQKFVIQIPSSDEAIRKRIPIRNPYGLPKTFRITTSNSDIVKITDSLLSVPPMGKLPCEMYFVKNTHLQKNIETLLYISDAETYVQEEAYSITLAFEAS

Target ORF information:

RefSeq Version	NM_073581.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans NePHronoPhthisis (human kidney disease) homolog (nphp-4), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_073581.3

ORF Insert Sequence:

ATGTCGGTCA ACGACTGGTA TTCGTTGTTT CTGGCAAACC GGCCAGTTGA AATGAAACGT 
AATGTTTCGA GAGGAACTAA AGCTTTGTGT TATTCAATGT TCATTTCCAA TTTGACATCT 
CCACAGCTGA CTGAGAACAT TCGATACCAG ATTAGTGCTT TTTTATTCGA CACGAAGACT 
TCTCAAATGT TTGGGAGGCA ATGTAGAACA GAATGGATTC CAGCGAATAG TAATGGAACT 
TGTGTTTTTA ATGAAACATT ATATTTTTAC TCAATTATAA ACAGCAGAGA TGTGCTTCTT 
ATTCTTGAAT TTGTGGAAGA AGGATCAGAC GAAATAACGC CTGCCACAAG CGTTGGATGG 
TTTTCAACGC ATATCGAGAA GAAAACGCCC GTAGAAATAT CAAACACTAA AATTTTCGAT 
ATATTTGGTG GAACTCCAAA ACTATTAATT TTTGATAAAG AAACAGTTTT AAAACCAGTT 
GGAAATGTTG AATGTACATA CAACATATTC GAAATGCCAC CCATATTCTT CCAATGTTTG 
CCTGAATTTT GCATTGTTTG CGACAAAGAC ATTATTCCTG GAATAATCAA AGACAGTTCT 
GATGAATGGT GGTTGAGCAC GCCAAAAGAG ATGCCAACAA TTCCCGCTGC AATTGATGCA 
ATTGTAATTC AATTCAAGAA TAATGTACCT GAATTAGAAA AACAAATTAC ACATGATATT 
GAAAAAGAAT GGGCATTGAA AGAAGGAGGA ACTCTTAAAC CAAAAGCAAT CATAATGGAT 
AGAAAATTGA GAATCGGAGT ACACAATGGG TACACATATG TCACTGAACC ATTCACAGTT 
GACTTGGAAA TAATCTCCTC AAATGCTGGT GATACTCTAA GATCGCGAAA GAAACCTATT 
GACTTTGGGA AATCATCAAA TTGGGAAGAA CAACTGCTAT TTCAAGCTGC TGGAAACCCG 
CGTCTGGCAC TTCGAAATCT GTATGCGGAT CCAAGAATGG CTATCATATT CTTGCTTGAA 
TACACCTTTC ACCGAGAAGA CAATCAAAGC CTTAATCAGA CTATTCTAAT CGGATGGGCT 
GCATGGACTC CATTCTCAGA TGGAGCCTTT TCTGGAAAAG AAGTGGAGAC ACGAGTTAGC 
TTTGTTGGTG GTCCACGTCC GAATCCAGAA GGCGTTTTAT GTTATAAGAA CGTATTGAAT 
CAACCAGACT CCTTGAAGCC ATTGAATGAG AAACTTGAAA TTTTTGTGGA CTTTAAATTT 
TATGAAAATG GAAGAAGTGT TCACAATACA CCAACATCAA GAAGGGCTGC TGACTCTGCA 
AGAGTTCAAA CTGGAAGATC AGGCGATAAT GGCCAATCTG CTAGATCAAA TAGAAAATCA 
GTGAAAATAG AAACTCCTAG AAGTCCAGAA AATTCGAATA GATTCCCTGC ACTAGTAGAT 
ACAGGAAGAT CAGTTTCATC AGTAGATGAA TTGAGATCAA TAAATGAGGA TTTGAATAGA 
TTCATAGAAG AACCAATGGA AATTCCAGTT CAAGACGTAG TTGTTGCAAA GAAACCAGTA 
GAAGAACCTC TACCAATAAC ATCAGTTTAC AAGATTCCTT TTGATGAGCT CAAACCAATA 
AACTTCCCAA GATCTGCCCA TTCCATGTTT GCGAGACAAA ACTTCACTCA GTTGAAAGAC 
AGGAACGGAT CACCACCAAA TACTGAAGAT GTGACACTAA AAACCATCAT CGATATGAAA 
AGAGAGCAAT TGGACAGACT CATTACATCA CATGTATATT TTCAATTTAT TGCTTTCAAA 
CAACTTGCTG CTCCTGATGC TCGCATGATT AAGAAGTTAT TTTTCACAAT CGGGTTTTAC 
CGATTCCCCG ATATTACAAC AGAGTCAATG CTTCTCACAT CTATGGAGAA AGGAGAACCA 
ACCCTTTTAA CCCGACTTGA TAAAAATGGG AATTCGGATG TTATAGCTTC ACCTGGATTC 
ATAGCAAAAT ATATAATCGA AGGAGAAGAA AGCAAGGCTG ACTTTTTGGA TTTTATGGCT 
TCTGGTCATG CAACTATTGA TGTATGGGAT TCAGACTCGT TGATTCACTT GGGATCAACT 
ATTGTGCCAA TCAAGAATTT GTATCGAAGA GGAAGGGAAG CTGTTCAGTT GTTTATTCAA 
TGCCCAGTGG TTGATACATC TTTAGACACT TCATCAAAAG CTGGGGCTTT CCTTTACATG 
AGAGTTGCCA ATATCGGATT TCCGTCAGGC AATACATATG ATCTCTCCTC CTCCTCTTCA 
TCGCTGACTA CCACAAGATC CAACGTAAAC TCTGGACAAG GTACAGTAGT CCGCCGACTG 
ACGTCATCAA TCCGGTTAAA TGAAGAAGGA CCCCATTCCT ATCGAATTCA CGCAAAACCA 
TTACCTGGTA ATAGTGGTGT TGGACTAGAT CGGTTTTTGA CAGCTCAGAG ACTAGATATT 
CAACAACGGC ATGAACAATT GTTCAATGAG AATAGTTTGG ATAAGATCAG ACAATGGAAT 
GATTTAAAAG AAGGATTCAA CTTTTCTGAT AATAAAGAAA TTGCACAGAA ATTCATTTTT 
GAAGAAGAGC TAGCTGCATA CAAAAAGCTT CGATATGAAA GTAAACCTGC AAAACTTCTT 
GAAGCTGTTT TCAAAGGTAT TACAAGTTGT CACCAAATAA ATCCCAGTTT TGGAGAGAAA 
GTATTTTTTG AATTTCCTCT CGAGAACTAC AATTCTGAAC CAATCAATTG CACCATTGAA 
TTTGATGATG AAGCTTTGAA ACCAGTATTT GATGCAGAAG AATGGAAATT TTACAAAACA 
GTGAACAAAG TAACAACTCC ATCAGAAAAG CAAATGATGA GACAAACAAC GGATAGAATT 
GAGATTTGTC TTCAACCTGG GGATGTTTTA TTTATTCCAT TTATCTACGA CGCTTTCTTT 
TTCCCAAATG ATGCATTCAA CATGTACTCA ACAAAAGTTG TATTTAGAAG ATGGGATACA 
AAAGAACCAC TAGCAATATT GGATCTCCAC GTGCATCGAA GAAATTTCCT TCTTCAACAT 
TCAGTTACTT TTATTTGTGA AACATCTGGA AACTGGGAAA AACAATTAGT ACTACCACCA 
ATGGCCAGAG ACAGAAGAGT TTTGTCATGT CGATGCTCTG ATCCGTCTGT AAGACTGACT 
GTTCGAAATG CGACACTACA ACAGATTGTT GGATTCACAA CATATTCAGG GGAAACGAAT 
GATCGAAAGA CGTTTCTTTT ATTGATGTAT AGTGATCATT ATCAAACAAG ATTAATGGCT 
ACCTGGAAAA TCACTATTCT TCCATTCTTC AATGTTGATG TTCGTAGTAT TGTTGGGCAA 
ACAACTCGTC TTCATTTATT AGTGCACAGA AGAAGCGAGC ATGATGGAGT GCCAGATGAT 
TTACTGAAAG TGTATACAGC ATCTGGATGT ATGAAAGTTG TGGATTCTGT GCTCACTGAA 
AGAACACCTA CAGCCACAAT TGATTTTACA CCAAACTTTA TTGGAACAAA GAAGCTTGTC 
GTTTCTGTTG TGAATACCAA TACATTGAAG TTAGAACGAG GGTTTTTGGT TTACGGAAAG 
TCTGAAGCGC CACGGATTAC GCAGAAGTTT GTCATACAAA TTCCATCATC TGATGAAGCT 
ATTAGAAAGA GAATCCCGAT AAGAAACCCA TATGGTTTAC CAAAGACATT CCGAATCACC 
ACATCAAATT CTGATATTGT AAAAATTACG GATAGTCTGT TGTCAGTTCC ACCTATGGGA 
AAACTTCCAT GTGAAATGTA TTTTGTGAAG AATACACATC TTCAAAAGAA TATAGAGACA 
TTGTTGTATA TATCAGATGC GGAGACTTAT GTACAAGAAG AAGCATATTC AATCACACTT 
GCATTCGAAG CTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nphp-4 ( NM_073581.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_505982.2 Caenorhabditis elegans NePHronoPhthisis (human kidney disease) homolog (nphp-4), partial mRNA.

Price & Availability↑

CloneID	OCa345044
Clone ID Related Accession (Same CDS sequence)	NM_073581.3 , NM_073581.2
Accession Version	NM_073581.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3918bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	NePHronoPhthisis (human kidney disease) homolog
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). On Aug 9, 2005 this sequence version replaced NM_073581.1. COMPLETENESS: incomplete on both ends.

ATGTCGGTCA ACGACTGGTA TTCGTTGTTT CTGGCAAACC GGCCAGTTGA AATGAAACGT 
AATGTTTCGA GAGGAACTAA AGCTTTGTGT TATTCAATGT TCATTTCCAA TTTGACATCT 
CCACAGCTGA CTGAGAACAT TCGATACCAG ATTAGTGCTT TTTTATTCGA CACGAAGACT 
TCTCAAATGT TTGGGAGGCA ATGTAGAACA GAATGGATTC CAGCGAATAG TAATGGAACT 
TGTGTTTTTA ATGAAACATT ATATTTTTAC TCAATTATAA ACAGCAGAGA TGTGCTTCTT 
ATTCTTGAAT TTGTGGAAGA AGGATCAGAC GAAATAACGC CTGCCACAAG CGTTGGATGG 
TTTTCAACGC ATATCGAGAA GAAAACGCCC GTAGAAATAT CAAACACTAA AATTTTCGAT 
ATATTTGGTG GAACTCCAAA ACTATTAATT TTTGATAAAG AAACAGTTTT AAAACCAGTT 
GGAAATGTTG AATGTACATA CAACATATTC GAAATGCCAC CCATATTCTT CCAATGTTTG 
CCTGAATTTT GCATTGTTTG CGACAAAGAC ATTATTCCTG GAATAATCAA AGACAGTTCT 
GATGAATGGT GGTTGAGCAC GCCAAAAGAG ATGCCAACAA TTCCCGCTGC AATTGATGCA 
ATTGTAATTC AATTCAAGAA TAATGTACCT GAATTAGAAA AACAAATTAC ACATGATATT 
GAAAAAGAAT GGGCATTGAA AGAAGGAGGA ACTCTTAAAC CAAAAGCAAT CATAATGGAT 
AGAAAATTGA GAATCGGAGT ACACAATGGG TACACATATG TCACTGAACC ATTCACAGTT 
GACTTGGAAA TAATCTCCTC AAATGCTGGT GATACTCTAA GATCGCGAAA GAAACCTATT 
GACTTTGGGA AATCATCAAA TTGGGAAGAA CAACTGCTAT TTCAAGCTGC TGGAAACCCG 
CGTCTGGCAC TTCGAAATCT GTATGCGGAT CCAAGAATGG CTATCATATT CTTGCTTGAA 
TACACCTTTC ACCGAGAAGA CAATCAAAGC CTTAATCAGA CTATTCTAAT CGGATGGGCT 
GCATGGACTC CATTCTCAGA TGGAGCCTTT TCTGGAAAAG AAGTGGAGAC ACGAGTTAGC 
TTTGTTGGTG GTCCACGTCC GAATCCAGAA GGCGTTTTAT GTTATAAGAA CGTATTGAAT 
CAACCAGACT CCTTGAAGCC ATTGAATGAG AAACTTGAAA TTTTTGTGGA CTTTAAATTT 
TATGAAAATG GAAGAAGTGT TCACAATACA CCAACATCAA GAAGGGCTGC TGACTCTGCA 
AGAGTTCAAA CTGGAAGATC AGGCGATAAT GGCCAATCTG CTAGATCAAA TAGAAAATCA 
GTGAAAATAG AAACTCCTAG AAGTCCAGAA AATTCGAATA GATTCCCTGC ACTAGTAGAT 
ACAGGAAGAT CAGTTTCATC AGTAGATGAA TTGAGATCAA TAAATGAGGA TTTGAATAGA 
TTCATAGAAG AACCAATGGA AATTCCAGTT CAAGACGTAG TTGTTGCAAA GAAACCAGTA 
GAAGAACCTC TACCAATAAC ATCAGTTTAC AAGATTCCTT TTGATGAGCT CAAACCAATA 
AACTTCCCAA GATCTGCCCA TTCCATGTTT GCGAGACAAA ACTTCACTCA GTTGAAAGAC 
AGGAACGGAT CACCACCAAA TACTGAAGAT GTGACACTAA AAACCATCAT CGATATGAAA 
AGAGAGCAAT TGGACAGACT CATTACATCA CATGTATATT TTCAATTTAT TGCTTTCAAA 
CAACTTGCTG CTCCTGATGC TCGCATGATT AAGAAGTTAT TTTTCACAAT CGGGTTTTAC 
CGATTCCCCG ATATTACAAC AGAGTCAATG CTTCTCACAT CTATGGAGAA AGGAGAACCA 
ACCCTTTTAA CCCGACTTGA TAAAAATGGG AATTCGGATG TTATAGCTTC ACCTGGATTC 
ATAGCAAAAT ATATAATCGA AGGAGAAGAA AGCAAGGCTG ACTTTTTGGA TTTTATGGCT 
TCTGGTCATG CAACTATTGA TGTATGGGAT TCAGACTCGT TGATTCACTT GGGATCAACT 
ATTGTGCCAA TCAAGAATTT GTATCGAAGA GGAAGGGAAG CTGTTCAGTT GTTTATTCAA 
TGCCCAGTGG TTGATACATC TTTAGACACT TCATCAAAAG CTGGGGCTTT CCTTTACATG 
AGAGTTGCCA ATATCGGATT TCCGTCAGGC AATACATATG ATCTCTCCTC CTCCTCTTCA 
TCGCTGACTA CCACAAGATC CAACGTAAAC TCTGGACAAG GTACAGTAGT CCGCCGACTG 
ACGTCATCAA TCCGGTTAAA TGAAGAAGGA CCCCATTCCT ATCGAATTCA CGCAAAACCA 
TTACCTGGTA ATAGTGGTGT TGGACTAGAT CGGTTTTTGA CAGCTCAGAG ACTAGATATT 
CAACAACGGC ATGAACAATT GTTCAATGAG AATAGTTTGG ATAAGATCAG ACAATGGAAT 
GATTTAAAAG AAGGATTCAA CTTTTCTGAT AATAAAGAAA TTGCACAGAA ATTCATTTTT 
GAAGAAGAGC TAGCTGCATA CAAAAAGCTT CGATATGAAA GTAAACCTGC AAAACTTCTT 
GAAGCTGTTT TCAAAGGTAT TACAAGTTGT CACCAAATAA ATCCCAGTTT TGGAGAGAAA 
GTATTTTTTG AATTTCCTCT CGAGAACTAC AATTCTGAAC CAATCAATTG CACCATTGAA 
TTTGATGATG AAGCTTTGAA ACCAGTATTT GATGCAGAAG AATGGAAATT TTACAAAACA 
GTGAACAAAG TAACAACTCC ATCAGAAAAG CAAATGATGA GACAAACAAC GGATAGAATT 
GAGATTTGTC TTCAACCTGG GGATGTTTTA TTTATTCCAT TTATCTACGA CGCTTTCTTT 
TTCCCAAATG ATGCATTCAA CATGTACTCA ACAAAAGTTG TATTTAGAAG ATGGGATACA 
AAAGAACCAC TAGCAATATT GGATCTCCAC GTGCATCGAA GAAATTTCCT TCTTCAACAT 
TCAGTTACTT TTATTTGTGA AACATCTGGA AACTGGGAAA AACAATTAGT ACTACCACCA 
ATGGCCAGAG ACAGAAGAGT TTTGTCATGT CGATGCTCTG ATCCGTCTGT AAGACTGACT 
GTTCGAAATG CGACACTACA ACAGATTGTT GGATTCACAA CATATTCAGG GGAAACGAAT 
GATCGAAAGA CGTTTCTTTT ATTGATGTAT AGTGATCATT ATCAAACAAG ATTAATGGCT 
ACCTGGAAAA TCACTATTCT TCCATTCTTC AATGTTGATG TTCGTAGTAT TGTTGGGCAA 
ACAACTCGTC TTCATTTATT AGTGCACAGA AGAAGCGAGC ATGATGGAGT GCCAGATGAT 
TTACTGAAAG TGTATACAGC ATCTGGATGT ATGAAAGTTG TGGATTCTGT GCTCACTGAA 
AGAACACCTA CAGCCACAAT TGATTTTACA CCAAACTTTA TTGGAACAAA GAAGCTTGTC 
GTTTCTGTTG TGAATACCAA TACATTGAAG TTAGAACGAG GGTTTTTGGT TTACGGAAAG 
TCTGAAGCGC CACGGATTAC GCAGAAGTTT GTCATACAAA TTCCATCATC TGATGAAGCT 
ATTAGAAAGA GAATCCCGAT AAGAAACCCA TATGGTTTAC CAAAGACATT CCGAATCACC 
ACATCAAATT CTGATATTGT AAAAATTACG GATAGTCTGT TGTCAGTTCC ACCTATGGGA 
AAACTTCCAT GTGAAATGTA TTTTGTGAAG AATACACATC TTCAAAAGAA TATAGAGACA 
TTGTTGTATA TATCAGATGC GGAGACTTAT GTACAAGAAG AAGCATATTC AATCACACTT 
GCATTCGAAG CTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_505982.2
CDS	1..3918
Translation	MSVNDWYSLFLANRPVEMKRNVSRGTKALCYSMFISNLTSPQLTENIRYQISAFLFDTKTSQMFGRQCRTEWIPANSNGTCVFNETLYFYSIINSRDVLLILEFVEEGSDEITPATSVGWFSTHIEKKTPVEISNTKIFDIFGGTPKLLIFDKETVLKPVGNVECTYNIFEMPPIFFQCLPEFCIVCDKDIIPGIIKDSSDEWWLSTPKEMPTIPAAIDAIVIQFKNNVPELEKQITHDIEKEWALKEGGTLKPKAIIMDRKLRIGVHNGYTYVTEPFTVDLEIISSNAGDTLRSRKKPIDFGKSSNWEEQLLFQAAGNPRLALRNLYADPRMAIIFLLEYTFHREDNQSLNQTILIGWAAWTPFSDGAFSGKEVETRVSFVGGPRPNPEGVLCYKNVLNQPDSLKPLNEKLEIFVDFKFYENGRSVHNTPTSRRAADSARVQTGRSGDNGQSARSNRKSVKIETPRSPENSNRFPALVDTGRSVSSVDELRSINEDLNRFIEEPMEIPVQDVVVAKKPVEEPLPITSVYKIPFDELKPINFPRSAHSMFARQNFTQLKDRNGSPPNTEDVTLKTIIDMKREQLDRLITSHVYFQFIAFKQLAAPDARMIKKLFFTIGFYRFPDITTESMLLTSMEKGEPTLLTRLDKNGNSDVIASPGFIAKYIIEGEESKADFLDFMASGHATIDVWDSDSLIHLGSTIVPIKNLYRRGREAVQLFIQCPVVDTSLDTSSKAGAFLYMRVANIGFPSGNTYDLSSSSSSLTTTRSNVNSGQGTVVRRLTSSIRLNEEGPHSYRIHAKPLPGNSGVGLDRFLTAQRLDIQQRHEQLFNENSLDKIRQWNDLKEGFNFSDNKEIAQKFIFEEELAAYKKLRYESKPAKLLEAVFKGITSCHQINPSFGEKVFFEFPLENYNSEPINCTIEFDDEALKPVFDAEEWKFYKTVNKVTTPSEKQMMRQTTDRIEICLQPGDVLFIPFIYDAFFFPNDAFNMYSTKVVFRRWDTKEPLAILDLHVHRRNFLLQHSVTFICETSGNWEKQLVLPPMARDRRVLSCRCSDPSVRLTVRNATLQQIVGFTTYSGETNDRKTFLLLMYSDHYQTRLMATWKITILPFFNVDVRSIVGQTTRLHLLVHRRSEHDGVPDDLLKVYTASGCMKVVDSVLTERTPTATIDFTPNFIGTKKLVVSVVNTNTLKLERGFLVYGKSEAPRITQKFVIQIPSSDEAIRKRIPIRNPYGLPKTFRITTSNSDIVKITDSLLSVPPMGKLPCEMYFVKNTHLQKNIETLLYISDAETYVQEEAYSITLAFEAS

Target ORF information:

RefSeq Version	NM_073581.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans NePHronoPhthisis (human kidney disease) homolog (nphp-4), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_073581.2

ORF Insert Sequence:

ATGTCGGTCA ACGACTGGTA TTCGTTGTTT CTGGCAAACC GGCCAGTTGA AATGAAACGT 
AATGTTTCGA GAGGAACTAA AGCTTTGTGT TATTCAATGT TCATTTCCAA TTTGACATCT 
CCACAGCTGA CTGAGAACAT TCGATACCAG ATTAGTGCTT TTTTATTCGA CACGAAGACT 
TCTCAAATGT TTGGGAGGCA ATGTAGAACA GAATGGATTC CAGCGAATAG TAATGGAACT 
TGTGTTTTTA ATGAAACATT ATATTTTTAC TCAATTATAA ACAGCAGAGA TGTGCTTCTT 
ATTCTTGAAT TTGTGGAAGA AGGATCAGAC GAAATAACGC CTGCCACAAG CGTTGGATGG 
TTTTCAACGC ATATCGAGAA GAAAACGCCC GTAGAAATAT CAAACACTAA AATTTTCGAT 
ATATTTGGTG GAACTCCAAA ACTATTAATT TTTGATAAAG AAACAGTTTT AAAACCAGTT 
GGAAATGTTG AATGTACATA CAACATATTC GAAATGCCAC CCATATTCTT CCAATGTTTG 
CCTGAATTTT GCATTGTTTG CGACAAAGAC ATTATTCCTG GAATAATCAA AGACAGTTCT 
GATGAATGGT GGTTGAGCAC GCCAAAAGAG ATGCCAACAA TTCCCGCTGC AATTGATGCA 
ATTGTAATTC AATTCAAGAA TAATGTACCT GAATTAGAAA AACAAATTAC ACATGATATT 
GAAAAAGAAT GGGCATTGAA AGAAGGAGGA ACTCTTAAAC CAAAAGCAAT CATAATGGAT 
AGAAAATTGA GAATCGGAGT ACACAATGGG TACACATATG TCACTGAACC ATTCACAGTT 
GACTTGGAAA TAATCTCCTC AAATGCTGGT GATACTCTAA GATCGCGAAA GAAACCTATT 
GACTTTGGGA AATCATCAAA TTGGGAAGAA CAACTGCTAT TTCAAGCTGC TGGAAACCCG 
CGTCTGGCAC TTCGAAATCT GTATGCGGAT CCAAGAATGG CTATCATATT CTTGCTTGAA 
TACACCTTTC ACCGAGAAGA CAATCAAAGC CTTAATCAGA CTATTCTAAT CGGATGGGCT 
GCATGGACTC CATTCTCAGA TGGAGCCTTT TCTGGAAAAG AAGTGGAGAC ACGAGTTAGC 
TTTGTTGGTG GTCCACGTCC GAATCCAGAA GGCGTTTTAT GTTATAAGAA CGTATTGAAT 
CAACCAGACT CCTTGAAGCC ATTGAATGAG AAACTTGAAA TTTTTGTGGA CTTTAAATTT 
TATGAAAATG GAAGAAGTGT TCACAATACA CCAACATCAA GAAGGGCTGC TGACTCTGCA 
AGAGTTCAAA CTGGAAGATC AGGCGATAAT GGCCAATCTG CTAGATCAAA TAGAAAATCA 
GTGAAAATAG AAACTCCTAG AAGTCCAGAA AATTCGAATA GATTCCCTGC ACTAGTAGAT 
ACAGGAAGAT CAGTTTCATC AGTAGATGAA TTGAGATCAA TAAATGAGGA TTTGAATAGA 
TTCATAGAAG AACCAATGGA AATTCCAGTT CAAGACGTAG TTGTTGCAAA GAAACCAGTA 
GAAGAACCTC TACCAATAAC ATCAGTTTAC AAGATTCCTT TTGATGAGCT CAAACCAATA 
AACTTCCCAA GATCTGCCCA TTCCATGTTT GCGAGACAAA ACTTCACTCA GTTGAAAGAC 
AGGAACGGAT CACCACCAAA TACTGAAGAT GTGACACTAA AAACCATCAT CGATATGAAA 
AGAGAGCAAT TGGACAGACT CATTACATCA CATGTATATT TTCAATTTAT TGCTTTCAAA 
CAACTTGCTG CTCCTGATGC TCGCATGATT AAGAAGTTAT TTTTCACAAT CGGGTTTTAC 
CGATTCCCCG ATATTACAAC AGAGTCAATG CTTCTCACAT CTATGGAGAA AGGAGAACCA 
ACCCTTTTAA CCCGACTTGA TAAAAATGGG AATTCGGATG TTATAGCTTC ACCTGGATTC 
ATAGCAAAAT ATATAATCGA AGGAGAAGAA AGCAAGGCTG ACTTTTTGGA TTTTATGGCT 
TCTGGTCATG CAACTATTGA TGTATGGGAT TCAGACTCGT TGATTCACTT GGGATCAACT 
ATTGTGCCAA TCAAGAATTT GTATCGAAGA GGAAGGGAAG CTGTTCAGTT GTTTATTCAA 
TGCCCAGTGG TTGATACATC TTTAGACACT TCATCAAAAG CTGGGGCTTT CCTTTACATG 
AGAGTTGCCA ATATCGGATT TCCGTCAGGC AATACATATG ATCTCTCCTC CTCCTCTTCA 
TCGCTGACTA CCACAAGATC CAACGTAAAC TCTGGACAAG GTACAGTAGT CCGCCGACTG 
ACGTCATCAA TCCGGTTAAA TGAAGAAGGA CCCCATTCCT ATCGAATTCA CGCAAAACCA 
TTACCTGGTA ATAGTGGTGT TGGACTAGAT CGGTTTTTGA CAGCTCAGAG ACTAGATATT 
CAACAACGGC ATGAACAATT GTTCAATGAG AATAGTTTGG ATAAGATCAG ACAATGGAAT 
GATTTAAAAG AAGGATTCAA CTTTTCTGAT AATAAAGAAA TTGCACAGAA ATTCATTTTT 
GAAGAAGAGC TAGCTGCATA CAAAAAGCTT CGATATGAAA GTAAACCTGC AAAACTTCTT 
GAAGCTGTTT TCAAAGGTAT TACAAGTTGT CACCAAATAA ATCCCAGTTT TGGAGAGAAA 
GTATTTTTTG AATTTCCTCT CGAGAACTAC AATTCTGAAC CAATCAATTG CACCATTGAA 
TTTGATGATG AAGCTTTGAA ACCAGTATTT GATGCAGAAG AATGGAAATT TTACAAAACA 
GTGAACAAAG TAACAACTCC ATCAGAAAAG CAAATGATGA GACAAACAAC GGATAGAATT 
GAGATTTGTC TTCAACCTGG GGATGTTTTA TTTATTCCAT TTATCTACGA CGCTTTCTTT 
TTCCCAAATG ATGCATTCAA CATGTACTCA ACAAAAGTTG TATTTAGAAG ATGGGATACA 
AAAGAACCAC TAGCAATATT GGATCTCCAC GTGCATCGAA GAAATTTCCT TCTTCAACAT 
TCAGTTACTT TTATTTGTGA AACATCTGGA AACTGGGAAA AACAATTAGT ACTACCACCA 
ATGGCCAGAG ACAGAAGAGT TTTGTCATGT CGATGCTCTG ATCCGTCTGT AAGACTGACT 
GTTCGAAATG CGACACTACA ACAGATTGTT GGATTCACAA CATATTCAGG GGAAACGAAT 
GATCGAAAGA CGTTTCTTTT ATTGATGTAT AGTGATCATT ATCAAACAAG ATTAATGGCT 
ACCTGGAAAA TCACTATTCT TCCATTCTTC AATGTTGATG TTCGTAGTAT TGTTGGGCAA 
ACAACTCGTC TTCATTTATT AGTGCACAGA AGAAGCGAGC ATGATGGAGT GCCAGATGAT 
TTACTGAAAG TGTATACAGC ATCTGGATGT ATGAAAGTTG TGGATTCTGT GCTCACTGAA 
AGAACACCTA CAGCCACAAT TGATTTTACA CCAAACTTTA TTGGAACAAA GAAGCTTGTC 
GTTTCTGTTG TGAATACCAA TACATTGAAG TTAGAACGAG GGTTTTTGGT TTACGGAAAG 
TCTGAAGCGC CACGGATTAC GCAGAAGTTT GTCATACAAA TTCCATCATC TGATGAAGCT 
ATTAGAAAGA GAATCCCGAT AAGAAACCCA TATGGTTTAC CAAAGACATT CCGAATCACC 
ACATCAAATT CTGATATTGT AAAAATTACG GATAGTCTGT TGTCAGTTCC ACCTATGGGA 
AAACTTCCAT GTGAAATGTA TTTTGTGAAG AATACACATC TTCAAAAGAA TATAGAGACA 
TTGTTGTATA TATCAGATGC GGAGACTTAT GTACAAGAAG AAGCATATTC AATCACACTT 
GCATTCGAAG CTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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nphp-4 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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