NPHP4 gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_015475180.1	XP_015330666.1	nephrocystin-4 isoform X3
XM_015466839.1	XP_015322325.1	nephrocystin-4 isoform X1
XM_015466842.1	XP_015322328.1	nephrocystin-4 isoform X3
XM_015466845.1	XP_015322331.1	nephrocystin-4 isoform X3
XM_024976773.1	XP_024832541.1	nephrocystin-4 isoform X1
XM_005217172.4	XP_005217229.1	nephrocystin-4 isoform X1
XM_015475179.1	XP_015330665.1	nephrocystin-4 isoform X3
XM_015475178.1	XP_015330664.1	nephrocystin-4 isoform X1
XM_015466841.1	XP_015322327.1	nephrocystin-4 isoform X2
XM_015466844.1	XP_015322330.1	nephrocystin-4 isoform X3
XM_015466846.1	XP_015322332.1	nephrocystin-4 isoform X3
XM_024976778.1	XP_024832546.1	nephrocystin-4 isoform X4
XM_005217172.3	XP_005217229.1	nephrocystin-4 isoform X3
XM_010813390.2	XP_010811692.1	nephrocystin-4 isoform X3
XM_024976775.1	XP_024832543.1	nephrocystin-4 isoform X1
XM_024976776.1	XP_024832544.1	nephrocystin-4 isoform X1
XM_024976777.1	XP_024832545.1	nephrocystin-4 isoform X3
XM_024976779.1	XP_024832547.1	nephrocystin-4 isoform X5
XM_010813384.2	XP_010811686.1	nephrocystin-4 isoform X2
XM_005217171.3	XP_005217228.1	nephrocystin-4 isoform X3
XM_024976772.1	XP_024832540.1	nephrocystin-4 isoform X1
XM_010813390.3	XP_010811692.1	nephrocystin-4 isoform X1
XM_015466843.1	XP_015322329.1	nephrocystin-4 isoform X3
XM_005217171.4	XP_005217228.1	nephrocystin-4 isoform X1
XM_015475180.2	XP_015330666.1	nephrocystin-4 isoform X1
XM_024976774.1	XP_024832542.1	nephrocystin-4 isoform X1
XM_010813384.3	XP_010811686.2	nephrocystin-4 isoform X2

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa08717	XM_024976778.1 Latest version!	Bos taurus nephrocystin 4 (NPHP4), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OBa223960	XM_010813384.3 Latest version!	Bos taurus nephrocystin 4 (NPHP4), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa223961	XM_024976777.1 Latest version!	Bos taurus nephrocystin 4 (NPHP4), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa223962	XM_024976779.1 Latest version!	Bos taurus nephrocystin 4 (NPHP4), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa92992	XM_015466839.1 Latest version!	Bos taurus nephronophthisis 4 (NPHP4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBa92992	XM_015475178.1 Latest version!	Bos taurus nephronophthisis 4 (NPHP4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBa27367	XM_015466843.1 Latest version!	Bos taurus nephronophthisis 4 (NPHP4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa27367	XM_015466845.1 Latest version!	Bos taurus nephronophthisis 4 (NPHP4), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa27367	XM_015466846.1 Latest version!	Bos taurus nephronophthisis 4 (NPHP4), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa27367	XM_015466842.1 Latest version!	Bos taurus nephronophthisis 4 (NPHP4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa27367	XM_005217171.4 Latest version!	Bos taurus nephrocystin 4 (NPHP4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa27367	XM_024976772.1 Latest version!	Bos taurus nephrocystin 4 (NPHP4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa27367	XM_024976774.1 Latest version!	Bos taurus nephrocystin 4 (NPHP4), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa27367	XM_024976773.1 Latest version!	Bos taurus nephrocystin 4 (NPHP4), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa27367	XM_010813390.3 Latest version!	Bos taurus nephrocystin 4 (NPHP4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa27367	XM_015475180.2 Latest version!	Bos taurus nephrocystin 4 (NPHP4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa27367	XM_005217172.4 Latest version!	Bos taurus nephrocystin 4 (NPHP4), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa27367	XM_024976776.1 Latest version!	Bos taurus nephrocystin 4 (NPHP4), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa27367	XM_024976775.1 Latest version!	Bos taurus nephrocystin 4 (NPHP4), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa27367	XM_015475179.1 Latest version!	Bos taurus nephronophthisis 4 (NPHP4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa27367	XM_015466844.1 Latest version!	Bos taurus nephronophthisis 4 (NPHP4), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa50068	XM_010813384.2 Latest version!	Bos taurus nephronophthisis 4 (NPHP4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa50068	XM_015466841.1 Latest version!	Bos taurus nephronophthisis 4 (NPHP4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
View Old Accession Versions >>
OBa27367	XM_005217172.3	Bos taurus nephronophthisis 4 (NPHP4), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa27367	XM_010813390.2	Bos taurus nephronophthisis 4 (NPHP4), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa27367	XM_005217171.3	Bos taurus nephronophthisis 4 (NPHP4), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa27367	XM_015475180.1	Bos taurus nephronophthisis 4 (NPHP4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
Hide Old Accession Versions >>

NPHP4 ( XM_024976778.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832546.1 Bos taurus nephrocystin 4 (NPHP4), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OBa08717
Clone ID Related Accession (Same CDS sequence)	XM_024976778.1
Accession Version	XM_024976778.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4113bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	nephrocystin-4 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACGACT GGCACAGGAT CTTTGACCAA AACCTGCTGG TCCCTCCGCA CCCACAGAGA 
GCACGCCAGC CTCTGAAGGA ATCGACGGCG TTTCAGTGTG TCCTGAAGTG GCTTGATGGC 
CCGCTAATTA AGCAGGGGGT GCTGGAGCTG CTATCGGAGG TGGGCTGCCA CCTGCGCGTG 
TCTCTCTTCG ATGTCACCTA TCGGCACTTC TTCGGGAGGA CGTGGAGGAG CTCGCCGAGG 
CCTGCAGAGC CGCTCACCAG GCAGCCGGCC AGGATCGTCT TCAATGAGCC CTTGTATTTT 
CACACGTCCT TGAACCACCC TCATATTGTG GCCGTGGTGG AGGTGGTCGT TGAAGGCAGA 
AGACGAGACG GGACCCTCCA GACTCTGTCC TGTGGGTTTG GAATTCTTCG GATCTTCAGC 
CACAAGCCGG AATCTCCAAC TTCCACTTCC CAGGACAAAC GGCTGAGACT GTATCATGGG 
AGCCCGCGAG CCCTCCTCCA CCCGCTCCTT CAGGACCCCA TAGAACAGAA CAAGCACATG 
ACCCTCATTG AGAACTGCAG CCTGCAGTAC ACGCTGAGGC CACACCCGCT CCTGGAGCCC 
GTGTTCCACC TTCTTCCCGA GAACTTGCTG GTGTCCGGTC TGCAGCAGAT ACCTGGCCTC 
CTTCCAGCTC ACGGGGAATC AGGAGATGCC CTCCGGAAGC CCCGCCTGCA GAAGCCTCTG 
ACGTGCTACC TGGATGATCT GTTCTTCACG CTGTATCCCT CGCTGGAGAA GTTCGAGGAG 
GAGCTTCTGG AACTCGTGAT CAGCGATTGC TTCCGGGAGG CGGGCAGCCC GCTGGACGTC 
GGCACCCTGG AGATCCTGGA GCGGCGCCTG TGCGTGGGCG TGCACAATGG GTTGGGCTTC 
GTGCAGCGGC CGCAGGTCCT CGTGCTGGTG CCCGAGCTGG AGGTGGCCTT GACACGCTCT 
GCCAGCTTCA GCCGGAAGGT CGGCTCCTCT TCCAAGGCCA GCTCTGGAAA CCAGGCGCTG 
GTCTTGAGAA GCCGTCTCCG ACTCCCTGAG ATGGTCCGTC ACCCCGCGTT CGCCATTGTC 
TTCCAGCTGG AGTACGTGTT TAACAGCCCC TCGGGGGCAG ACAGCAATGT GAAGCAGGTG 
GAGTCAGGGA CGGTCCAGTT CCAGTACTCG CTGAGCCCTG AAGAACACCC AGGCACACCC 
ACAGCGAATG CAAGCAGCCC CAAGGCAGAG CGGCGGTCTT TTAGGAAGCC ACCCGTGTCC 
CCTTCAAGTC CACCAGCATC GCCGTCCCAG GGGCTCACCG CTGCCCAGGA CTCACCCATG 
GGACCAGGGC TGTCACTTTC TCAGCTGGCG GCTTCCCCAC CATCCCCAGC TCCCCGCCGC 
CGCTCGGCCA AACCTTCGTC ACAGCCACCT GGGAGCCCTG TGCCCTCCCT GGTCCCAGGG 
GAGTTCCCCT TGGAGGGCAG GATCTCTCAC CTGGAGGCTG ACCTGAGCCA GACAGCCTTA 
GCCCTGGGAA CGTCCGCTGC TGACCACTTG CAGGAACTGC CTTTCACACC CGTGCACGCC 
CCGATCATTG CAGGAACCCA GACCAGGAGC TCTGGAGGGA AGCTGTCACG GGCATCCCTG 
GCGCTGCTGC AGTCCGCTGG CTTTCCCGAG ATTCTGGACG CCAACAAGCA ACCAGCTGAG 
GCTGTGAGTC CTACAGACCC CGTGAAGTTC AGCCCTCAGA AGGAAGAATC AGACTGTCTA 
CAAGGCAATG AGATAGTGTT GCAATTTCTT GCCTTTAGCA GGGTGCCCCA GGATGAGCAG 
GCAGCGCCAT GGCCACAGAC TGTGTACTTC ACCTTCCAAT TCTACCGCTT CCCACCCGAG 
ACCACGCCAC GGCTGCAGCT CGTGGAGCTG GCCGATGCTG GGAAGACCAG CTCTGCCTCC 
CTGTCGCACA TCCTCGCCCA GATCCAGAAG GATGGCTCCT TTGATGCGGG GTCTCCGGGC 
TTTCAGCGGA AGTACCTGGT GGACCCCGGG TTCCTGAGAC CCGGGGAGCA GCGCTGGTTC 
GCCCGCTACC TGGCCATGCA GACCCTGCAG ATCGACGTCT GGGATGGGGA CTCCCTGCTT 
CTCATTGGAT CTGCTGCCGT TCAGCTGAAG CACCTTCTCC GCCAGGGGCG GCCGGCCGTG 
CAGGTCTCGC ATGAGCTGGA GGTTGTGGCG ACTGAATACG AGCAGGACGC CATGGTGGTG 
AGTGGAGACA TGACCAAGTT TGGCTGCGTC AGACCCATCG ACGTCCACAC GGTGGTGAAG 
GGCCAGCTGC ACTTGACCTT GGCCAACGTG GGTCACTTGT GTGAACAGAG AGTAAGGAGT 
CCCAGTTCCC TGCCACCTTC CAGGTCGAGG GTCATCTCAA ATGATGGAGT TGGCAACTTC 
ATGGGAGGCA GCCTCCTGAC ACCCGGGGGC CCCAGATCAG TGAAAAATGT GGTCCAAGCT 
CAGAAGCTGG CTGATGTGGA CAGCGAACTG GCCGCCCTGC TCTTCACACA ACCGAGACCA 
GGGGCCCGGG GGCCCCAGGG CATCAATCAA GAGGGGGATG CCGTCCGCAG GCGCAAGCTG 
GAGCGCATGA GGGCCGTGCG GCTGCGGGAA TGTGGATTGG AGCCGGGCCG CCGCGGGCTG 
GGCGCGCTGG CCCAGCAGAG CCTCCATGCC CAGCACGCCC GAGACCTGCA GGTCATTGCC 
GCCTACCGTG AGCGCACAAA GGCGGAGAGC ATCGCCAGCG CACTGAGCAC GGCCATCACC 
ACGCAGCACA CCATCCACGC CACCCTAGGC GCTGCCGAGT TCTTCGAGTT CGCCCTGAGG 
AACCCCCACA ACGCGCAGCA CATGGTGACC ATCGAGATAG ACAACCCCGA GCTCAGTCTC 
ATCCTGGATG GTCAGGAGTG GCGGTACTTC AAGGACATGG CTGACTTGCA CACACCCCTG 
GAGGAGGACA TGTTCCACCT GCGGGGCAGC CTGGCCCCCC AACTCTTCCT GCGACCTCGG 
GAGACTGCCC ACATCCCCTT CAAGTTCCAG ACTTTCTCTG TGGGCCCCTG GGCCTCGGCA 
CAGGCCTCTG CTGAGCTGAG ATCAGAGAAG GACATGGATG CCGGATCACC CTATAAGTCC 
AGCATGATGC CCACCAAACA CGCCAAGGTC CTGTTCCGCG CGGGCAGCGG CAAGCCCCTG 
GCCGTGCTCT GCCTGACGGT GGAGCTGCAG CCTCACGTGG TGGACCAGGT CTTCCGCTTC 
CACCACCCGG AGCTCACCTT CCTGAAGAAA GCCATCCGCC TGCCACCCTG GCACACGCTT 
CCAGGTGCCC CTGTAGGGAC TTCCAGTGAG GACCCCCCTT TCCATGTTCG ATGTAGTGAC 
CCGAACATCA TTTGTGAGAC ACAGAGTGTG GGCCCCGGGG AGCCCCGTGA CGTGTTCCTG 
AAGGTGGCCA GTGGCCCTAG CCCAGAGGTC AAGGACTTCT TTGTCACCAT CTACGTAGAC 
CGCTGGTTAG CAACACCTGT CCAGACCTGG CAGGTCCACC TGCACTCGCT GCAGCGAGTG 
GACGTCTCCT GTATCACTGG CCAGAGGACC CGCCTGGCTC TCGTCCTCCG GGGGACGCAG 
ACAGTTAGGA GAGTGCAGGC TTTCAGCTCA CACCCCCAGG AGCTGACGAC GGACCCCAGA 
GGTGTGTTCG TGCTGCCTCC TCACGGGGTG CAGGACTTGC ATGTGAGCGT CAGGCCCTGC 
AGGCCGGGCA GCCGCTTCGC GCACCTCCAC CTGGTTGACG TGGACGCCCA CCAGCTGGTG 
GCCTCGTGGC TCCTGTGTCT CTCCTGCCGG CCGCCTCTCA TCTCCAAGGC CTTCGAGATA 
AAGCTGCCCG CGGGGAGCGG GAGGGGCGCC AATAAGCGCA TCACCTACAC CAATCCCTAC 
CCCTCGCGGA GGGCCTACCG CCTGCACAGC GACCACCCCC ACCTGCTGCA GTTCAAGGAG 
GACGCCTTCC AGGTCGGGGG AGGTGAGACC TATACCATCG GCCTGCGGTT TGTGTCCCGT 
CAGAGAGCCG GGGAGGAAGA GATCCTGATT TACATCAACG ACCACGAAGA CAAAAACGAG 
GAGACCTTTT GCGTGAAGGT CATCTACCAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832546.1
CDS	281..4393
Translation	MHDWHRIFDQNLLVPPHPQRARQPLKESTAFQCVLKWLDGPLIKQGVLELLSEVGCHLRVSLFDVTYRHFFGRTWRSSPRPAEPLTRQPARIVFNEPLYFHTSLNHPHIVAVVEVVVEGRRRDGTLQTLSCGFGILRIFSHKPESPTSTSQDKRLRLYHGSPRALLHPLLQDPIEQNKHMTLIENCSLQYTLRPHPLLEPVFHLLPENLLVSGLQQIPGLLPAHGESGDALRKPRLQKPLTCYLDDLFFTLYPSLEKFEEELLELVISDCFREAGSPLDVGTLEILERRLCVGVHNGLGFVQRPQVLVLVPELEVALTRSASFSRKVGSSSKASSGNQALVLRSRLRLPEMVRHPAFAIVFQLEYVFNSPSGADSNVKQVESGTVQFQYSLSPEEHPGTPTANASSPKAERRSFRKPPVSPSSPPASPSQGLTAAQDSPMGPGLSLSQLAASPPSPAPRRRSAKPSSQPPGSPVPSLVPGEFPLEGRISHLEADLSQTALALGTSAADHLQELPFTPVHAPIIAGTQTRSSGGKLSRASLALLQSAGFPEILDANKQPAEAVSPTDPVKFSPQKEESDCLQGNEIVLQFLAFSRVPQDEQAAPWPQTVYFTFQFYRFPPETTPRLQLVELADAGKTSSASLSHILAQIQKDGSFDAGSPGFQRKYLVDPGFLRPGEQRWFARYLAMQTLQIDVWDGDSLLLIGSAAVQLKHLLRQGRPAVQVSHELEVVATEYEQDAMVVSGDMTKFGCVRPIDVHTVVKGQLHLTLANVGHLCEQRVRSPSSLPPSRSRVISNDGVGNFMGGSLLTPGGPRSVKNVVQAQKLADVDSELAALLFTQPRPGARGPQGINQEGDAVRRRKLERMRAVRLRECGLEPGRRGLGALAQQSLHAQHARDLQVIAAYRERTKAESIASALSTAITTQHTIHATLGAAEFFEFALRNPHNAQHMVTIEIDNPELSLILDGQEWRYFKDMADLHTPLEEDMFHLRGSLAPQLFLRPRETAHIPFKFQTFSVGPWASAQASAELRSEKDMDAGSPYKSSMMPTKHAKVLFRAGSGKPLAVLCLTVELQPHVVDQVFRFHHPELTFLKKAIRLPPWHTLPGAPVGTSSEDPPFHVRCSDPNIICETQSVGPGEPRDVFLKVASGPSPEVKDFFVTIYVDRWLATPVQTWQVHLHSLQRVDVSCITGQRTRLALVLRGTQTVRRVQAFSSHPQELTTDPRGVFVLPPHGVQDLHVSVRPCRPGSRFAHLHLVDVDAHQLVASWLLCLSCRPPLISKAFEIKLPAGSGRGANKRITYTNPYPSRRAYRLHSDHPHLLQFKEDAFQVGGGETYTIGLRFVSRQRAGEEEILIYINDHEDKNEETFCVKVIYQ

Target ORF information:

RefSeq Version	XM_024976778.1
Organism	Bos taurus(cattle)
Definition	Bos taurus nephrocystin 4 (NPHP4), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976778.1

ORF Insert Sequence:

ATGCACGACT GGCACAGGAT CTTTGACCAA AACCTGCTGG TCCCTCCGCA CCCACAGAGA 
GCACGCCAGC CTCTGAAGGA ATCGACGGCG TTTCAGTGTG TCCTGAAGTG GCTTGATGGC 
CCGCTAATTA AGCAGGGGGT GCTGGAGCTG CTATCGGAGG TGGGCTGCCA CCTGCGCGTG 
TCTCTCTTCG ATGTCACCTA TCGGCACTTC TTCGGGAGGA CGTGGAGGAG CTCGCCGAGG 
CCTGCAGAGC CGCTCACCAG GCAGCCGGCC AGGATCGTCT TCAATGAGCC CTTGTATTTT 
CACACGTCCT TGAACCACCC TCATATTGTG GCCGTGGTGG AGGTGGTCGT TGAAGGCAGA 
AGACGAGACG GGACCCTCCA GACTCTGTCC TGTGGGTTTG GAATTCTTCG GATCTTCAGC 
CACAAGCCGG AATCTCCAAC TTCCACTTCC CAGGACAAAC GGCTGAGACT GTATCATGGG 
AGCCCGCGAG CCCTCCTCCA CCCGCTCCTT CAGGACCCCA TAGAACAGAA CAAGCACATG 
ACCCTCATTG AGAACTGCAG CCTGCAGTAC ACGCTGAGGC CACACCCGCT CCTGGAGCCC 
GTGTTCCACC TTCTTCCCGA GAACTTGCTG GTGTCCGGTC TGCAGCAGAT ACCTGGCCTC 
CTTCCAGCTC ACGGGGAATC AGGAGATGCC CTCCGGAAGC CCCGCCTGCA GAAGCCTCTG 
ACGTGCTACC TGGATGATCT GTTCTTCACG CTGTATCCCT CGCTGGAGAA GTTCGAGGAG 
GAGCTTCTGG AACTCGTGAT CAGCGATTGC TTCCGGGAGG CGGGCAGCCC GCTGGACGTC 
GGCACCCTGG AGATCCTGGA GCGGCGCCTG TGCGTGGGCG TGCACAATGG GTTGGGCTTC 
GTGCAGCGGC CGCAGGTCCT CGTGCTGGTG CCCGAGCTGG AGGTGGCCTT GACACGCTCT 
GCCAGCTTCA GCCGGAAGGT CGGCTCCTCT TCCAAGGCCA GCTCTGGAAA CCAGGCGCTG 
GTCTTGAGAA GCCGTCTCCG ACTCCCTGAG ATGGTCCGTC ACCCCGCGTT CGCCATTGTC 
TTCCAGCTGG AGTACGTGTT TAACAGCCCC TCGGGGGCAG ACAGCAATGT GAAGCAGGTG 
GAGTCAGGGA CGGTCCAGTT CCAGTACTCG CTGAGCCCTG AAGAACACCC AGGCACACCC 
ACAGCGAATG CAAGCAGCCC CAAGGCAGAG CGGCGGTCTT TTAGGAAGCC ACCCGTGTCC 
CCTTCAAGTC CACCAGCATC GCCGTCCCAG GGGCTCACCG CTGCCCAGGA CTCACCCATG 
GGACCAGGGC TGTCACTTTC TCAGCTGGCG GCTTCCCCAC CATCCCCAGC TCCCCGCCGC 
CGCTCGGCCA AACCTTCGTC ACAGCCACCT GGGAGCCCTG TGCCCTCCCT GGTCCCAGGG 
GAGTTCCCCT TGGAGGGCAG GATCTCTCAC CTGGAGGCTG ACCTGAGCCA GACAGCCTTA 
GCCCTGGGAA CGTCCGCTGC TGACCACTTG CAGGAACTGC CTTTCACACC CGTGCACGCC 
CCGATCATTG CAGGAACCCA GACCAGGAGC TCTGGAGGGA AGCTGTCACG GGCATCCCTG 
GCGCTGCTGC AGTCCGCTGG CTTTCCCGAG ATTCTGGACG CCAACAAGCA ACCAGCTGAG 
GCTGTGAGTC CTACAGACCC CGTGAAGTTC AGCCCTCAGA AGGAAGAATC AGACTGTCTA 
CAAGGCAATG AGATAGTGTT GCAATTTCTT GCCTTTAGCA GGGTGCCCCA GGATGAGCAG 
GCAGCGCCAT GGCCACAGAC TGTGTACTTC ACCTTCCAAT TCTACCGCTT CCCACCCGAG 
ACCACGCCAC GGCTGCAGCT CGTGGAGCTG GCCGATGCTG GGAAGACCAG CTCTGCCTCC 
CTGTCGCACA TCCTCGCCCA GATCCAGAAG GATGGCTCCT TTGATGCGGG GTCTCCGGGC 
TTTCAGCGGA AGTACCTGGT GGACCCCGGG TTCCTGAGAC CCGGGGAGCA GCGCTGGTTC 
GCCCGCTACC TGGCCATGCA GACCCTGCAG ATCGACGTCT GGGATGGGGA CTCCCTGCTT 
CTCATTGGAT CTGCTGCCGT TCAGCTGAAG CACCTTCTCC GCCAGGGGCG GCCGGCCGTG 
CAGGTCTCGC ATGAGCTGGA GGTTGTGGCG ACTGAATACG AGCAGGACGC CATGGTGGTG 
AGTGGAGACA TGACCAAGTT TGGCTGCGTC AGACCCATCG ACGTCCACAC GGTGGTGAAG 
GGCCAGCTGC ACTTGACCTT GGCCAACGTG GGTCACTTGT GTGAACAGAG AGTAAGGAGT 
CCCAGTTCCC TGCCACCTTC CAGGTCGAGG GTCATCTCAA ATGATGGAGT TGGCAACTTC 
ATGGGAGGCA GCCTCCTGAC ACCCGGGGGC CCCAGATCAG TGAAAAATGT GGTCCAAGCT 
CAGAAGCTGG CTGATGTGGA CAGCGAACTG GCCGCCCTGC TCTTCACACA ACCGAGACCA 
GGGGCCCGGG GGCCCCAGGG CATCAATCAA GAGGGGGATG CCGTCCGCAG GCGCAAGCTG 
GAGCGCATGA GGGCCGTGCG GCTGCGGGAA TGTGGATTGG AGCCGGGCCG CCGCGGGCTG 
GGCGCGCTGG CCCAGCAGAG CCTCCATGCC CAGCACGCCC GAGACCTGCA GGTCATTGCC 
GCCTACCGTG AGCGCACAAA GGCGGAGAGC ATCGCCAGCG CACTGAGCAC GGCCATCACC 
ACGCAGCACA CCATCCACGC CACCCTAGGC GCTGCCGAGT TCTTCGAGTT CGCCCTGAGG 
AACCCCCACA ACGCGCAGCA CATGGTGACC ATCGAGATAG ACAACCCCGA GCTCAGTCTC 
ATCCTGGATG GTCAGGAGTG GCGGTACTTC AAGGACATGG CTGACTTGCA CACACCCCTG 
GAGGAGGACA TGTTCCACCT GCGGGGCAGC CTGGCCCCCC AACTCTTCCT GCGACCTCGG 
GAGACTGCCC ACATCCCCTT CAAGTTCCAG ACTTTCTCTG TGGGCCCCTG GGCCTCGGCA 
CAGGCCTCTG CTGAGCTGAG ATCAGAGAAG GACATGGATG CCGGATCACC CTATAAGTCC 
AGCATGATGC CCACCAAACA CGCCAAGGTC CTGTTCCGCG CGGGCAGCGG CAAGCCCCTG 
GCCGTGCTCT GCCTGACGGT GGAGCTGCAG CCTCACGTGG TGGACCAGGT CTTCCGCTTC 
CACCACCCGG AGCTCACCTT CCTGAAGAAA GCCATCCGCC TGCCACCCTG GCACACGCTT 
CCAGGTGCCC CTGTAGGGAC TTCCAGTGAG GACCCCCCTT TCCATGTTCG ATGTAGTGAC 
CCGAACATCA TTTGTGAGAC ACAGAGTGTG GGCCCCGGGG AGCCCCGTGA CGTGTTCCTG 
AAGGTGGCCA GTGGCCCTAG CCCAGAGGTC AAGGACTTCT TTGTCACCAT CTACGTAGAC 
CGCTGGTTAG CAACACCTGT CCAGACCTGG CAGGTCCACC TGCACTCGCT GCAGCGAGTG 
GACGTCTCCT GTATCACTGG CCAGAGGACC CGCCTGGCTC TCGTCCTCCG GGGGACGCAG 
ACAGTTAGGA GAGTGCAGGC TTTCAGCTCA CACCCCCAGG AGCTGACGAC GGACCCCAGA 
GGTGTGTTCG TGCTGCCTCC TCACGGGGTG CAGGACTTGC ATGTGAGCGT CAGGCCCTGC 
AGGCCGGGCA GCCGCTTCGC GCACCTCCAC CTGGTTGACG TGGACGCCCA CCAGCTGGTG 
GCCTCGTGGC TCCTGTGTCT CTCCTGCCGG CCGCCTCTCA TCTCCAAGGC CTTCGAGATA 
AAGCTGCCCG CGGGGAGCGG GAGGGGCGCC AATAAGCGCA TCACCTACAC CAATCCCTAC 
CCCTCGCGGA GGGCCTACCG CCTGCACAGC GACCACCCCC ACCTGCTGCA GTTCAAGGAG 
GACGCCTTCC AGGTCGGGGG AGGTGAGACC TATACCATCG GCCTGCGGTT TGTGTCCCGT 
CAGAGAGCCG GGGAGGAAGA GATCCTGATT TACATCAACG ACCACGAAGA CAAAAACGAG 
GAGACCTTTT GCGTGAAGGT CATCTACCAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPHP4 ( XM_010813384.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010811686.2 Bos taurus nephrocystin 4 (NPHP4), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OBa223960
Clone ID Related Accession (Same CDS sequence)	XM_010813384.3
Accession Version	XM_010813384.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4293bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	nephrocystin-4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010813384.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACGACT GGCACAGGAT CTTTGACCAA AACCTGCTGG TCCCTCCGCA CCCACAGAGA 
GCACGCCAGC CTCTGAAGGA ATCGACGGCG TTTCAGTGTG TCCTGAAGTG GCTTGATGGC 
CCGCTAATTA AGCAGGGGGT GCTGGAGCTG CTATCGGAGG TGGGCTGCCA CCTGCGCGTG 
TCTCTCTTCG ATGTCACCTA TCGGCACTTC TTCGGGAGGA CGTGGAGGAG CTCGCCGAGG 
CCTGCAGAGC CGCTCACCAG GCAGCCGGCC AGGATCGTCT TCAATGAGCC CTTGTATTTT 
CACACGTCCT TGAACCACCC TCATATTGTG GCCGTGGTGG AGGTGGTCGT TGAAGGCAGA 
AGACGAGACG GGACCCTCCA GACTCTGTCC TGTGGGTTTG GAATTCTTCG GATCTTCAGC 
CACAAGCCGG AATCTCCAAC TTCCACTTCC CAGGACAAAC GGCTGAGACT GTATCATGGG 
AGCCCGCGAG CCCTCCTCCA CCCGCTCCTT CAGGACCCCA TAGAACAGAA CAAGCACATG 
ACCCTCATTG AGAACTGCAG CCTGCAGTAC ACGCTGAGGC CACACCCGCT CCTGGAGCCC 
GTGTTCCACC TTCTTCCCGA GAACTTGCTG GTGTCCGGTC TGCAGCAGAT ACCTGGCCTC 
CTTCCAGCTC ACGGGGAATC AGGAGATGCC CTCCGGAAGC CCCGCCTGCA GAAGCCTCTG 
ACGTGCTACC TGGATGATCT GTTCTTCACG CTGTATCCCT CGCTGGAGAA GTTCGAGGAG 
GAGCTTCTGG AACTCGTGAT CAGCGATTGC TTCCGGGAGG CGGGCAGCCC GCTGGACGTC 
GGCACCCTGG AGATCCTGGA GCGGCGCCTG TGCGTGGGCG TGCACAATGG GTTGGGCTTC 
GTGCAGCGGC CGCAGGTCCT CGTGCTGGTG CCCGAGCTGG AGGTGGCCTT GACACGCTCT 
GCCAGCTTCA GCCGGAAGGT CGGCTCCTCT TCCAAGGCCA GCTCTGGAAA CCAGGCGCTG 
GTCTTGAGAA GCCGTCTCCG ACTCCCTGAG ATGGTCCGTC ACCCCGCGTT CGCCATTGTC 
TTCCAGCTGG AGTACGTGTT TAACAGCCCC TCGGGGGCAG ACAGCAATGC AGCTTCACTC 
TCCTCCTTGT CCAGCCTGGC ATGTATGCAC ATGGTTCGCT GGGCTGTCTG GAACCCCTCG 
CTGGAAGCCA GCTCCGGGCA GGTGATGTTA CCTCTCCAGG GTGGCATCCA GTCCAACCCT 
TCGCACTGTC TGGTCTATAA GGTACCCCCA GCCAGCATAA GCTCCGAAGA GGTGAAGCAG 
GTGGAGTCAG GGACGGTCCA GTTCCAGTAC TCGCTGAGCC CTGAAGAACA CCCAGGCACA 
CCCACAGCGA ATGCAAGCAG CCCCAAGGCA GAGCGGCGGT CTTTTAGGAA GCCACCCGTG 
TCCCCTTCAA GTCCACCAGC ATCGCCGTCC CAGGGGCTCA CCGCTGCCCA GGACTCACCC 
ATGGGACCAG GGCTGTCACT TTCTCAGCTG GCGGCTTCCC CACCATCCCC AGCTCCCCGC 
CGCCGCTCGG CCAAACCTTC GTCACAGCCA CCTGGGAGCC CTGTGCCCTC CCTGGTCCCA 
GGGGAGTTCC CCTTGGAGGG CAGGATCTCT CACCTGGAGG CTGACCTGAG CCAGACAGCC 
TTAGCCCTGG GAACGTCCGC TGCTGACCAC TTGCAGGAAC TGCCTTTCAC ACCCGTGCAC 
GCCCCGATCA TTGCAGGAAC CCAGACCAGG AGCTCTGGAG GGAAGCTGTC ACGGGCATCC 
CTGGCGCTGC TGCAGTCCGC TGGCTTTCCC GAGATTCTGG ACGCCAACAA GCAACCAGCT 
GAGGCTGTGA GTCCTACAGA CCCCGTGAAG TTCAGCCCTC AGAAGGAAGA ATCAGACTGT 
CTACAAGGCA ATGAGATAGT GTTGCAATTT CTTGCCTTTA GCAGGGTGCC CCAGGATGAG 
CAGGCAGCGC CATGGCCACA GACTGTGTAC TTCACCTTCC AATTCTACCG CTTCCCACCC 
GAGACCACGC CACGGCTGCA GCTCGTGGAG CTGGCCGATG CTGGGAAGAC CAGCTCTGCC 
TCCCTGTCGC ACATCCTCGC CCAGATCCAG AAGGATGGCT CCTTTGATGC GGGGTCTCCG 
GGCTTTCAGC GGAAGTACCT GGTGGACCCC GGGTTCCTGA GACCCGGGGA GCAGCGCTGG 
TTCGCCCGCT ACCTGGCCAT GCAGACCCTG CAGATCGACG TCTGGGATGG GGACTCCCTG 
CTTCTCATTG GATCTGCTGC CGTTCAGCTG AAGCACCTTC TCCGCCAGGG GCGGCCGGCC 
GTGCAGGTCT CGCATGAGCT GGAGGTTGTG GCGACTGAAT ACGAGCAGGA CGCCATGGTG 
GTGAGTGGAG ACATGACCAA GTTTGGCTGC GTCAGACCCA TCGACGTCCA CACGGTGGTG 
AAGGGCCAGC TGCACTTGAC CTTGGCCAAC GTGGGTCACT TGTGTGAACA GAGAGTAAGG 
AGTCCCAGTT CCCTGCCACC TTCCAGGTCG AGGGTCATCT CAAATGATGG AGTTGGCAAC 
TTCATGGGAG GCAGCCTCCT GACACCCGGG GGCCCCAGAT TGAAAAATGT GGTCCAAGCT 
CAGAAGCTGG CTGATGTGGA CAGCGAACTG GCCGCCCTGC TCTTCACACA ACCGAGACCA 
GGGGCCCGGG GGCCCCAGGG CATCAATCAA GAGGGGGATG CCGTCCGCAG GCGCAAGCTG 
GAGCGCATGA GGGCCGTGCG GCTGCGGGAA TGTGGATTGG AGCCGGGCCG CCGCGGGCTG 
GGCGCGCTGG CCCAGCAGAG CCTCCATGCC CAGCACGCCC GAGACCTGCA GGTCATTGCC 
GCCTACCGTG AGCGCACAAA GGCGGAGAGC ATCGCCAGCG CACTGAGCAC GGCCATCACC 
ACGCAGCACA CCATCCACGC CACCCTAGGC GCTGCCGAGT TCTTCGAGTT CGCCCTGAGG 
AACCCCCACA ACGCGCAGCA CATGGTGACC ATCGAGATAG ACAACCCCGA GCTCAGTCTC 
ATCCTGGATG GTCAGGAGTG GCGGTACTTC AAGGACATGG CTGACTTGCA CACACCCCTG 
GAGGAGGACA TGTTCCACCT GCGGGGCAGC CTGGCCCCCC AACTCTTCCT GCGACCTCGG 
GAGACTGCCC ACATCCCCTT CAAGTTCCAG ACTTTCTCTG TGGGCCCCTG GGCCTCGGCA 
CAGGCCTCTG CTGAGCTGAG ATCAGAGAAG GACATGGATG CCGGATCACC CTATAAGTCC 
AGCATGATGC CCACCAAACA CGCCAAGGTC CTGTTCCGCG CGGGCAGCGG CAAGCCCCTG 
GCCGTGCTCT GCCTGACGGT GGAGCTGCAG CCTCACGTGG TGGACCAGGT CTTCCGCTTC 
CACCACCCGG AGCTCACCTT CCTGAAGAAA GCCATCCGCC TGCCACCCTG GCACACGCTT 
CCAGGTGCCC CTGTAGGGAC TTCCAGTGAG GACCCCCCTT TCCATGTTCG ATGTAGTGAC 
CCGAACATCA TTTGTGAGAC ACAGAGTGTG GGCCCCGGGG AGCCCCGTGA CGTGTTCCTG 
AAGGTGGCCA GTGGCCCTAG CCCAGAGGTC AAGGACTTCT TTGTCACCAT CTACGTAGAC 
CGCTGGTTAG CAACACCTGT CCAGACCTGG CAGGTCCACC TGCACTCGCT GCAGCGAGTG 
GACGTCTCCT GTATCACTGG CCAGAGGACC CGCCTGGCTC TCGTCCTCCG GGGGACGCAG 
ACAGTTAGGA GAGTGCAGGC TTTCAGCTCA CACCCCCAGG AGCTGACGAC GGACCCCAGA 
GGTGTGTTCG TGCTGCCTCC TCACGGGGTG CAGGACTTGC ATGTGAGCGT CAGGCCCTGC 
AGGCCGGGCA GCCGCTTCGC GCACCTCCAC CTGGTTGACG TGGACGCCCA CCAGCTGGTG 
GCCTCGTGGC TCCTGTGTCT CTCCTGCCGG CCGCCTCTCA TCTCCAAGGC CTTCGAGATA 
AAGCTGCCCG CGGGGAGCGG GAGGGGCGCC AATAAGCGCA TCACCTACAC CAATCCCTAC 
CCCTCGCGGA GGGCCTACCG CCTGCACAGC GACCACCCCC ACCTGCTGCA GTTCAAGGAG 
GACGCCTTCC AGGTCGGGGG AGGTGAGACC TATACCATCG GCCTGCGGTT TGTGTCCCGT 
CAGAGAGCCG GGGAGGAAGA GATCCTGATT TACATCAACG ACCACGAAGA CAAAAACGAG 
GAGACCTTTT GCGTGAAGGT CATCTACCAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010811686.2
CDS	281..4573
Translation	MHDWHRIFDQNLLVPPHPQRARQPLKESTAFQCVLKWLDGPLIKQGVLELLSEVGCHLRVSLFDVTYRHFFGRTWRSSPRPAEPLTRQPARIVFNEPLYFHTSLNHPHIVAVVEVVVEGRRRDGTLQTLSCGFGILRIFSHKPESPTSTSQDKRLRLYHGSPRALLHPLLQDPIEQNKHMTLIENCSLQYTLRPHPLLEPVFHLLPENLLVSGLQQIPGLLPAHGESGDALRKPRLQKPLTCYLDDLFFTLYPSLEKFEEELLELVISDCFREAGSPLDVGTLEILERRLCVGVHNGLGFVQRPQVLVLVPELEVALTRSASFSRKVGSSSKASSGNQALVLRSRLRLPEMVRHPAFAIVFQLEYVFNSPSGADSNAASLSSLSSLACMHMVRWAVWNPSLEASSGQVMLPLQGGIQSNPSHCLVYKVPPASISSEEVKQVESGTVQFQYSLSPEEHPGTPTANASSPKAERRSFRKPPVSPSSPPASPSQGLTAAQDSPMGPGLSLSQLAASPPSPAPRRRSAKPSSQPPGSPVPSLVPGEFPLEGRISHLEADLSQTALALGTSAADHLQELPFTPVHAPIIAGTQTRSSGGKLSRASLALLQSAGFPEILDANKQPAEAVSPTDPVKFSPQKEESDCLQGNEIVLQFLAFSRVPQDEQAAPWPQTVYFTFQFYRFPPETTPRLQLVELADAGKTSSASLSHILAQIQKDGSFDAGSPGFQRKYLVDPGFLRPGEQRWFARYLAMQTLQIDVWDGDSLLLIGSAAVQLKHLLRQGRPAVQVSHELEVVATEYEQDAMVVSGDMTKFGCVRPIDVHTVVKGQLHLTLANVGHLCEQRVRSPSSLPPSRSRVISNDGVGNFMGGSLLTPGGPRLKNVVQAQKLADVDSELAALLFTQPRPGARGPQGINQEGDAVRRRKLERMRAVRLRECGLEPGRRGLGALAQQSLHAQHARDLQVIAAYRERTKAESIASALSTAITTQHTIHATLGAAEFFEFALRNPHNAQHMVTIEIDNPELSLILDGQEWRYFKDMADLHTPLEEDMFHLRGSLAPQLFLRPRETAHIPFKFQTFSVGPWASAQASAELRSEKDMDAGSPYKSSMMPTKHAKVLFRAGSGKPLAVLCLTVELQPHVVDQVFRFHHPELTFLKKAIRLPPWHTLPGAPVGTSSEDPPFHVRCSDPNIICETQSVGPGEPRDVFLKVASGPSPEVKDFFVTIYVDRWLATPVQTWQVHLHSLQRVDVSCITGQRTRLALVLRGTQTVRRVQAFSSHPQELTTDPRGVFVLPPHGVQDLHVSVRPCRPGSRFAHLHLVDVDAHQLVASWLLCLSCRPPLISKAFEIKLPAGSGRGANKRITYTNPYPSRRAYRLHSDHPHLLQFKEDAFQVGGGETYTIGLRFVSRQRAGEEEILIYINDHEDKNEETFCVKVIYQ

Target ORF information:

RefSeq Version	XM_010813384.3
Organism	Bos taurus(cattle)
Definition	Bos taurus nephrocystin 4 (NPHP4), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010813384.3

ORF Insert Sequence:

ATGCACGACT GGCACAGGAT CTTTGACCAA AACCTGCTGG TCCCTCCGCA CCCACAGAGA 
GCACGCCAGC CTCTGAAGGA ATCGACGGCG TTTCAGTGTG TCCTGAAGTG GCTTGATGGC 
CCGCTAATTA AGCAGGGGGT GCTGGAGCTG CTATCGGAGG TGGGCTGCCA CCTGCGCGTG 
TCTCTCTTCG ATGTCACCTA TCGGCACTTC TTCGGGAGGA CGTGGAGGAG CTCGCCGAGG 
CCTGCAGAGC CGCTCACCAG GCAGCCGGCC AGGATCGTCT TCAATGAGCC CTTGTATTTT 
CACACGTCCT TGAACCACCC TCATATTGTG GCCGTGGTGG AGGTGGTCGT TGAAGGCAGA 
AGACGAGACG GGACCCTCCA GACTCTGTCC TGTGGGTTTG GAATTCTTCG GATCTTCAGC 
CACAAGCCGG AATCTCCAAC TTCCACTTCC CAGGACAAAC GGCTGAGACT GTATCATGGG 
AGCCCGCGAG CCCTCCTCCA CCCGCTCCTT CAGGACCCCA TAGAACAGAA CAAGCACATG 
ACCCTCATTG AGAACTGCAG CCTGCAGTAC ACGCTGAGGC CACACCCGCT CCTGGAGCCC 
GTGTTCCACC TTCTTCCCGA GAACTTGCTG GTGTCCGGTC TGCAGCAGAT ACCTGGCCTC 
CTTCCAGCTC ACGGGGAATC AGGAGATGCC CTCCGGAAGC CCCGCCTGCA GAAGCCTCTG 
ACGTGCTACC TGGATGATCT GTTCTTCACG CTGTATCCCT CGCTGGAGAA GTTCGAGGAG 
GAGCTTCTGG AACTCGTGAT CAGCGATTGC TTCCGGGAGG CGGGCAGCCC GCTGGACGTC 
GGCACCCTGG AGATCCTGGA GCGGCGCCTG TGCGTGGGCG TGCACAATGG GTTGGGCTTC 
GTGCAGCGGC CGCAGGTCCT CGTGCTGGTG CCCGAGCTGG AGGTGGCCTT GACACGCTCT 
GCCAGCTTCA GCCGGAAGGT CGGCTCCTCT TCCAAGGCCA GCTCTGGAAA CCAGGCGCTG 
GTCTTGAGAA GCCGTCTCCG ACTCCCTGAG ATGGTCCGTC ACCCCGCGTT CGCCATTGTC 
TTCCAGCTGG AGTACGTGTT TAACAGCCCC TCGGGGGCAG ACAGCAATGC AGCTTCACTC 
TCCTCCTTGT CCAGCCTGGC ATGTATGCAC ATGGTTCGCT GGGCTGTCTG GAACCCCTCG 
CTGGAAGCCA GCTCCGGGCA GGTGATGTTA CCTCTCCAGG GTGGCATCCA GTCCAACCCT 
TCGCACTGTC TGGTCTATAA GGTACCCCCA GCCAGCATAA GCTCCGAAGA GGTGAAGCAG 
GTGGAGTCAG GGACGGTCCA GTTCCAGTAC TCGCTGAGCC CTGAAGAACA CCCAGGCACA 
CCCACAGCGA ATGCAAGCAG CCCCAAGGCA GAGCGGCGGT CTTTTAGGAA GCCACCCGTG 
TCCCCTTCAA GTCCACCAGC ATCGCCGTCC CAGGGGCTCA CCGCTGCCCA GGACTCACCC 
ATGGGACCAG GGCTGTCACT TTCTCAGCTG GCGGCTTCCC CACCATCCCC AGCTCCCCGC 
CGCCGCTCGG CCAAACCTTC GTCACAGCCA CCTGGGAGCC CTGTGCCCTC CCTGGTCCCA 
GGGGAGTTCC CCTTGGAGGG CAGGATCTCT CACCTGGAGG CTGACCTGAG CCAGACAGCC 
TTAGCCCTGG GAACGTCCGC TGCTGACCAC TTGCAGGAAC TGCCTTTCAC ACCCGTGCAC 
GCCCCGATCA TTGCAGGAAC CCAGACCAGG AGCTCTGGAG GGAAGCTGTC ACGGGCATCC 
CTGGCGCTGC TGCAGTCCGC TGGCTTTCCC GAGATTCTGG ACGCCAACAA GCAACCAGCT 
GAGGCTGTGA GTCCTACAGA CCCCGTGAAG TTCAGCCCTC AGAAGGAAGA ATCAGACTGT 
CTACAAGGCA ATGAGATAGT GTTGCAATTT CTTGCCTTTA GCAGGGTGCC CCAGGATGAG 
CAGGCAGCGC CATGGCCACA GACTGTGTAC TTCACCTTCC AATTCTACCG CTTCCCACCC 
GAGACCACGC CACGGCTGCA GCTCGTGGAG CTGGCCGATG CTGGGAAGAC CAGCTCTGCC 
TCCCTGTCGC ACATCCTCGC CCAGATCCAG AAGGATGGCT CCTTTGATGC GGGGTCTCCG 
GGCTTTCAGC GGAAGTACCT GGTGGACCCC GGGTTCCTGA GACCCGGGGA GCAGCGCTGG 
TTCGCCCGCT ACCTGGCCAT GCAGACCCTG CAGATCGACG TCTGGGATGG GGACTCCCTG 
CTTCTCATTG GATCTGCTGC CGTTCAGCTG AAGCACCTTC TCCGCCAGGG GCGGCCGGCC 
GTGCAGGTCT CGCATGAGCT GGAGGTTGTG GCGACTGAAT ACGAGCAGGA CGCCATGGTG 
GTGAGTGGAG ACATGACCAA GTTTGGCTGC GTCAGACCCA TCGACGTCCA CACGGTGGTG 
AAGGGCCAGC TGCACTTGAC CTTGGCCAAC GTGGGTCACT TGTGTGAACA GAGAGTAAGG 
AGTCCCAGTT CCCTGCCACC TTCCAGGTCG AGGGTCATCT CAAATGATGG AGTTGGCAAC 
TTCATGGGAG GCAGCCTCCT GACACCCGGG GGCCCCAGAT TGAAAAATGT GGTCCAAGCT 
CAGAAGCTGG CTGATGTGGA CAGCGAACTG GCCGCCCTGC TCTTCACACA ACCGAGACCA 
GGGGCCCGGG GGCCCCAGGG CATCAATCAA GAGGGGGATG CCGTCCGCAG GCGCAAGCTG 
GAGCGCATGA GGGCCGTGCG GCTGCGGGAA TGTGGATTGG AGCCGGGCCG CCGCGGGCTG 
GGCGCGCTGG CCCAGCAGAG CCTCCATGCC CAGCACGCCC GAGACCTGCA GGTCATTGCC 
GCCTACCGTG AGCGCACAAA GGCGGAGAGC ATCGCCAGCG CACTGAGCAC GGCCATCACC 
ACGCAGCACA CCATCCACGC CACCCTAGGC GCTGCCGAGT TCTTCGAGTT CGCCCTGAGG 
AACCCCCACA ACGCGCAGCA CATGGTGACC ATCGAGATAG ACAACCCCGA GCTCAGTCTC 
ATCCTGGATG GTCAGGAGTG GCGGTACTTC AAGGACATGG CTGACTTGCA CACACCCCTG 
GAGGAGGACA TGTTCCACCT GCGGGGCAGC CTGGCCCCCC AACTCTTCCT GCGACCTCGG 
GAGACTGCCC ACATCCCCTT CAAGTTCCAG ACTTTCTCTG TGGGCCCCTG GGCCTCGGCA 
CAGGCCTCTG CTGAGCTGAG ATCAGAGAAG GACATGGATG CCGGATCACC CTATAAGTCC 
AGCATGATGC CCACCAAACA CGCCAAGGTC CTGTTCCGCG CGGGCAGCGG CAAGCCCCTG 
GCCGTGCTCT GCCTGACGGT GGAGCTGCAG CCTCACGTGG TGGACCAGGT CTTCCGCTTC 
CACCACCCGG AGCTCACCTT CCTGAAGAAA GCCATCCGCC TGCCACCCTG GCACACGCTT 
CCAGGTGCCC CTGTAGGGAC TTCCAGTGAG GACCCCCCTT TCCATGTTCG ATGTAGTGAC 
CCGAACATCA TTTGTGAGAC ACAGAGTGTG GGCCCCGGGG AGCCCCGTGA CGTGTTCCTG 
AAGGTGGCCA GTGGCCCTAG CCCAGAGGTC AAGGACTTCT TTGTCACCAT CTACGTAGAC 
CGCTGGTTAG CAACACCTGT CCAGACCTGG CAGGTCCACC TGCACTCGCT GCAGCGAGTG 
GACGTCTCCT GTATCACTGG CCAGAGGACC CGCCTGGCTC TCGTCCTCCG GGGGACGCAG 
ACAGTTAGGA GAGTGCAGGC TTTCAGCTCA CACCCCCAGG AGCTGACGAC GGACCCCAGA 
GGTGTGTTCG TGCTGCCTCC TCACGGGGTG CAGGACTTGC ATGTGAGCGT CAGGCCCTGC 
AGGCCGGGCA GCCGCTTCGC GCACCTCCAC CTGGTTGACG TGGACGCCCA CCAGCTGGTG 
GCCTCGTGGC TCCTGTGTCT CTCCTGCCGG CCGCCTCTCA TCTCCAAGGC CTTCGAGATA 
AAGCTGCCCG CGGGGAGCGG GAGGGGCGCC AATAAGCGCA TCACCTACAC CAATCCCTAC 
CCCTCGCGGA GGGCCTACCG CCTGCACAGC GACCACCCCC ACCTGCTGCA GTTCAAGGAG 
GACGCCTTCC AGGTCGGGGG AGGTGAGACC TATACCATCG GCCTGCGGTT TGTGTCCCGT 
CAGAGAGCCG GGGAGGAAGA GATCCTGATT TACATCAACG ACCACGAAGA CAAAAACGAG 
GAGACCTTTT GCGTGAAGGT CATCTACCAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPHP4 ( XM_024976777.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832545.1 Bos taurus nephrocystin 4 (NPHP4), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OBa223961
Clone ID Related Accession (Same CDS sequence)	XM_024976777.1
Accession Version	XM_024976777.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4266bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	nephrocystin-4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACGACT GGCACAGGAT CTTTGACCAA AACCTGCTGG TCCCTCCGCA CCCACAGAGA 
GCACGCCAGC CTCTGAAGGA ATCGACGGCG TTTCAGTGTG TCCTGAAGTG GCTTGATGGC 
CCGCTAATTA AGCAGGGGGT GCTGGAGCTG CTATCGGAGG TGGGCTGCCA CCTGCGCGTG 
TCTCTCTTCG ATGTCACCTA TCGGCACTTC TTCGGGAGGA CGTGGAGGAG CTCGCCGAGG 
CCTGCAGAGC CGCTCACCAG GCAGCCGGCC AGGATCGTCT TCAATGAGCC CTTGTATTTT 
CACACGTCCT TGAACCACCC TCATATTGTG GCCGTGGTGG AGGTGGTCGT TGAAGGCAGA 
AGACGAGACG GGACCCTCCA GACTCTGTCC TGTGGGTTTG GAATTCTTCG GATCTTCAGC 
CACAAGCCGG AATCTCCAAC TTCCACTTCC CAGGACAAAC GGCTGAGACT GTATCATGGG 
AGCCCGCGAG CCCTCCTCCA CCCGCTCCTT CAGGACCCCA TAGAACAGAA CAAGCACATG 
ACCCTCATTG AGAACTGCAG CCTGCAGTAC ACGCTGAGGC CACACCCGCT CCTGGAGCCC 
GTGTTCCACC TTCTTCCCGA GAACTTGCTG GTGTCCGGTC TGCAGCAGAT ACCTGGCCTC 
CTTCCAGCTC ACGGGGAATC AGGAGATGCC CTCCGGAAGC CCCGCCTGCA GAAGCCTCTG 
ACGTGCTACC TGGATGATCT GTTCTTCACG CTGTATCCCT CGCTGGAGAA GTTCGAGGAG 
GAGCTTCTGG AACTCGTGAT CAGCGATTGC TTCCGGGAGG CGGGCAGCCC GCTGGACGTC 
GGCACCCTGG AGATCCTGGA GCGGCGCCTG TGCGTGGGCG TGCACAATGG GTTGGGCTTC 
GTGCAGCGGC CGCAGGTCCT CGTGCTGGTG CCCGAGCTGG AGGTGGCCTT GACACGCTCT 
GCCAGCTTCA GCCGGAAGGT CGGCTCCTCT TCCAAGGCCA GCTCTGGAAA CCAGGCGCTG 
GTCTTGAGAA GCCGTCTCCG ACTCCCTGAG ATGGTCCGTC ACCCCGCGTT CGCCATTGTC 
TTCCAGCTGG AGTACGTGTT TAACAGCCCC TCGGGGGCAG ACAGCAATGC AGCTTCACTC 
TCCTCCTTGT CCAGCCTGGC ATGTATGCAC ATGGTTCGCT GGGCTGTCTG GAACCCCTCG 
CTGGAAGCCA GCTCCGGGCA GGTGATGTTA CCTCTCCAGG GTGGCATCCA GTCCAACCCT 
TCGCACTGTC TGGTCTATAA GGTACCCCCA GCCAGCATAA GCTCCGAAGA GGTGAAGCAG 
GTGGAGTCAG GGACGGTCCA GTTCCAGTAC TCGCTGAGCC CTGAAGAACA CCCAGGCACA 
CCCACAGCGA ATGCAAGCAG CCCCAAGGCA GAGCGGCGGT CTTTTAGGAA GCCACCCGTG 
TCCCCTTCAA GTAAGTGTGT GCTGAGAACT GAGTCTTCAA GCTCATGGCA CAGCTCCATG 
TGTGCCCAGG TCCACCAGCA TCGCCGTCCC AGGGGCTCAC CGCTGCCCAG GACTCACCCA 
TGGGACCAGG GCTGTCACTT TCTCAGCTGG CGGCTTCCCC ACCATCCCCA GCTCCCCGCC 
GCCGCTCGGC CAAACCTTCG TCACAGCCAC CTGGGAGCCC TGTGCCCTCC CTGGTCCCAG 
GGGAACCCAG ACCAGGAGTC GGCCTTCTTC TGGAGGCTGG CTCAGGGGGC CATCCGTTCC 
AGCTCTGGAG GGAAGCTGTC ACGGGCATCC CTGGCGCTGC TGCAGTCCGC TGGCTTTCCC 
GAGATTCTGG ACGCCAACAA GCAACCAGCT GAGGCTGTGA GTCCTACAGA CCCCGTGAAG 
TTCAGCCCTC AGAAGGAAGA ATCAGACTGT CTACAAGGCA ATGAGATAGT GTTGCAATTT 
CTTGCCTTTA GCAGGGTGCC CCAGGATGAG CAGGCAGCGC CATGGCCACA GACTGTGTAC 
TTCACCTTCC AATTCTACCG CTTCCCACCC GAGACCACGC CACGGCTGCA GCTCGTGGAG 
CTGGCCGATG CTGGGAAGAC CAGCTCTGCC TCCCTGTCGC ACATCCTCGC CCAGATCCAG 
AAGGATGGCT CCTTTGATGC GGGGTCTCCG GGCTTTCAGC GGAAGTACCT GGTGGACCCC 
GGGTTCCTGA GACCCGGGGA GCAGCGCTGG TTCGCCCGCT ACCTGGCCAT GCAGACCCTG 
CAGATCGACG TCTGGGATGG GGACTCCCTG CTTCTCATTG GATCTGCTGC CGTTCAGCTG 
AAGCACCTTC TCCGCCAGGG GCGGCCGGCC GTGCAGGTCT CGCATGAGCT GGAGGTTGTG 
GCGACTGAAT ACGAGCAGGA CGCCATGGTG GTGAGTGGAG ACATGACCAA GTTTGGCTGC 
GTCAGACCCA TCGACGTCCA CACGGTGGTG AAGGGCCAGC TGCACTTGAC CTTGGCCAAC 
GTGGGTCACT TGTGTGAACA GAGAGTAAGG AGTCCCAGTT CCCTGCCACC TTCCAGGTCG 
AGGGTCATCT CAAATGATGG AGTTGGCAAC TTCATGGGAG GCAGCCTCCT GACACCCGGG 
GGCCCCAGAT CAGTGAAAAA TGTGGTCCAA GCTCAGAAGC TGGCTGATGT GGACAGCGAA 
CTGGCCGCCC TGCTCTTCAC ACAACCGAGA CCAGGGGCCC GGGGGCCCCA GGGCATCAAT 
CAAGAGGGGG ATGCCGTCCG CAGGCGCAAG CTGGAGCGCA TGAGGGCCGT GCGGCTGCGG 
GAATGTGGAT TGGAGCCGGG CCGCCGCGGG CTGGGCGCGC TGGCCCAGCA GAGCCTCCAT 
GCCCAGCACG CCCGAGACCT GCAGGTCATT GCCGCCTACC GTGAGCGCAC AAAGGCGGAG 
AGCATCGCCA GCGCACTGAG CACGGCCATC ACCACGCAGC ACACCATCCA CGCCACCCTA 
GGCGCTGCCG AGTTCTTCGA GTTCGCCCTG AGGAACCCCC ACAACGCGCA GCACATGGTG 
ACCATCGAGA TAGACAACCC CGAGCTCAGT CTCATCCTGG ATGGTCAGGA GTGGCGGTAC 
TTCAAGGACA TGGCTGACTT GCACACACCC CTGGAGGAGG ACATGTTCCA CCTGCGGGGC 
AGCCTGGCCC CCCAACTCTT CCTGCGACCT CGGGAGACTG CCCACATCCC CTTCAAGTTC 
CAGACTTTCT CTGTGGGCCC CTGGGCCTCG GCACAGGCCT CTGCTGAGCT GAGATCAGAG 
AAGGACATGG ATGCCGGATC ACCCTATAAG TCCAGCATGA TGCCCACCAA ACACGCCAAG 
GTCCTGTTCC GCGCGGGCAG CGGCAAGCCC CTGGCCGTGC TCTGCCTGAC GGTGGAGCTG 
CAGCCTCACG TGGTGGACCA GGTCTTCCGC TTCCACCACC CGGAGCTCAC CTTCCTGAAG 
AAAGCCATCC GCCTGCCACC CTGGCACACG CTTCCAGGTG CCCCTGTAGG GACTTCCAGT 
GAGGACCCCC CTTTCCATGT TCGATGTAGT GACCCGAACA TCATTTGTGA GACACAGAGT 
GTGGGCCCCG GGGAGCCCCG TGACGTGTTC CTGAAGGTGG CCAGTGGCCC TAGCCCAGAG 
GTCAAGGACT TCTTTGTCAC CATCTACGTA GACCGCTGGT TAGCAACACC TGTCCAGACC 
TGGCAGGTCC ACCTGCACTC GCTGCAGCGA GTGGACGTCT CCTGTATCAC TGGCCAGAGG 
ACCCGCCTGG CTCTCGTCCT CCGGGGGACG CAGACAGTTA GGAGAGTGCA GGCTTTCAGC 
TCACACCCCC AGGAGCTGAC GACGGACCCC AGAGGTGTGT TCGTGCTGCC TCCTCACGGG 
GTGCAGGACT TGCATGTGAG CGTCAGGCCC TGCAGGCCGG GCAGCCGCTT CGCGCACCTC 
CACCTGGTTG ACGTGGACGC CCACCAGCTG GTGGCCTCGT GGCTCCTGTG TCTCTCCTGC 
CGGCCGCCTC TCATCTCCAA GGCCTTCGAG ATAAAGCTGC CCGCGGGGAG CGGGAGGGGC 
GCCAATAAGC GCATCACCTA CACCAATCCC TACCCCTCGC GGAGGGCCTA CCGCCTGCAC 
AGCGACCACC CCCACCTGCT GCAGTTCAAG GAGGACGCCT TCCAGGTCGG GGGAGGTGAG 
ACCTATACCA TCGGCCTGCG GTTTGTGTCC CGTCAGAGAG CCGGGGAGGA AGAGATCCTG 
ATTTACATCA ACGACCACGA AGACAAAAAC GAGGAGACCT TTTGCGTGAA GGTCATCTAC 
CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832545.1
CDS	281..4546
Translation	MHDWHRIFDQNLLVPPHPQRARQPLKESTAFQCVLKWLDGPLIKQGVLELLSEVGCHLRVSLFDVTYRHFFGRTWRSSPRPAEPLTRQPARIVFNEPLYFHTSLNHPHIVAVVEVVVEGRRRDGTLQTLSCGFGILRIFSHKPESPTSTSQDKRLRLYHGSPRALLHPLLQDPIEQNKHMTLIENCSLQYTLRPHPLLEPVFHLLPENLLVSGLQQIPGLLPAHGESGDALRKPRLQKPLTCYLDDLFFTLYPSLEKFEEELLELVISDCFREAGSPLDVGTLEILERRLCVGVHNGLGFVQRPQVLVLVPELEVALTRSASFSRKVGSSSKASSGNQALVLRSRLRLPEMVRHPAFAIVFQLEYVFNSPSGADSNAASLSSLSSLACMHMVRWAVWNPSLEASSGQVMLPLQGGIQSNPSHCLVYKVPPASISSEEVKQVESGTVQFQYSLSPEEHPGTPTANASSPKAERRSFRKPPVSPSSKCVLRTESSSSWHSSMCAQVHQHRRPRGSPLPRTHPWDQGCHFLSWRLPHHPQLPAAARPNLRHSHLGALCPPWSQGNPDQESAFFWRLAQGAIRSSSGGKLSRASLALLQSAGFPEILDANKQPAEAVSPTDPVKFSPQKEESDCLQGNEIVLQFLAFSRVPQDEQAAPWPQTVYFTFQFYRFPPETTPRLQLVELADAGKTSSASLSHILAQIQKDGSFDAGSPGFQRKYLVDPGFLRPGEQRWFARYLAMQTLQIDVWDGDSLLLIGSAAVQLKHLLRQGRPAVQVSHELEVVATEYEQDAMVVSGDMTKFGCVRPIDVHTVVKGQLHLTLANVGHLCEQRVRSPSSLPPSRSRVISNDGVGNFMGGSLLTPGGPRSVKNVVQAQKLADVDSELAALLFTQPRPGARGPQGINQEGDAVRRRKLERMRAVRLRECGLEPGRRGLGALAQQSLHAQHARDLQVIAAYRERTKAESIASALSTAITTQHTIHATLGAAEFFEFALRNPHNAQHMVTIEIDNPELSLILDGQEWRYFKDMADLHTPLEEDMFHLRGSLAPQLFLRPRETAHIPFKFQTFSVGPWASAQASAELRSEKDMDAGSPYKSSMMPTKHAKVLFRAGSGKPLAVLCLTVELQPHVVDQVFRFHHPELTFLKKAIRLPPWHTLPGAPVGTSSEDPPFHVRCSDPNIICETQSVGPGEPRDVFLKVASGPSPEVKDFFVTIYVDRWLATPVQTWQVHLHSLQRVDVSCITGQRTRLALVLRGTQTVRRVQAFSSHPQELTTDPRGVFVLPPHGVQDLHVSVRPCRPGSRFAHLHLVDVDAHQLVASWLLCLSCRPPLISKAFEIKLPAGSGRGANKRITYTNPYPSRRAYRLHSDHPHLLQFKEDAFQVGGGETYTIGLRFVSRQRAGEEEILIYINDHEDKNEETFCVKVIYQ

Target ORF information:

RefSeq Version	XM_024976777.1
Organism	Bos taurus(cattle)
Definition	Bos taurus nephrocystin 4 (NPHP4), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976777.1

ORF Insert Sequence:

ATGCACGACT GGCACAGGAT CTTTGACCAA AACCTGCTGG TCCCTCCGCA CCCACAGAGA 
GCACGCCAGC CTCTGAAGGA ATCGACGGCG TTTCAGTGTG TCCTGAAGTG GCTTGATGGC 
CCGCTAATTA AGCAGGGGGT GCTGGAGCTG CTATCGGAGG TGGGCTGCCA CCTGCGCGTG 
TCTCTCTTCG ATGTCACCTA TCGGCACTTC TTCGGGAGGA CGTGGAGGAG CTCGCCGAGG 
CCTGCAGAGC CGCTCACCAG GCAGCCGGCC AGGATCGTCT TCAATGAGCC CTTGTATTTT 
CACACGTCCT TGAACCACCC TCATATTGTG GCCGTGGTGG AGGTGGTCGT TGAAGGCAGA 
AGACGAGACG GGACCCTCCA GACTCTGTCC TGTGGGTTTG GAATTCTTCG GATCTTCAGC 
CACAAGCCGG AATCTCCAAC TTCCACTTCC CAGGACAAAC GGCTGAGACT GTATCATGGG 
AGCCCGCGAG CCCTCCTCCA CCCGCTCCTT CAGGACCCCA TAGAACAGAA CAAGCACATG 
ACCCTCATTG AGAACTGCAG CCTGCAGTAC ACGCTGAGGC CACACCCGCT CCTGGAGCCC 
GTGTTCCACC TTCTTCCCGA GAACTTGCTG GTGTCCGGTC TGCAGCAGAT ACCTGGCCTC 
CTTCCAGCTC ACGGGGAATC AGGAGATGCC CTCCGGAAGC CCCGCCTGCA GAAGCCTCTG 
ACGTGCTACC TGGATGATCT GTTCTTCACG CTGTATCCCT CGCTGGAGAA GTTCGAGGAG 
GAGCTTCTGG AACTCGTGAT CAGCGATTGC TTCCGGGAGG CGGGCAGCCC GCTGGACGTC 
GGCACCCTGG AGATCCTGGA GCGGCGCCTG TGCGTGGGCG TGCACAATGG GTTGGGCTTC 
GTGCAGCGGC CGCAGGTCCT CGTGCTGGTG CCCGAGCTGG AGGTGGCCTT GACACGCTCT 
GCCAGCTTCA GCCGGAAGGT CGGCTCCTCT TCCAAGGCCA GCTCTGGAAA CCAGGCGCTG 
GTCTTGAGAA GCCGTCTCCG ACTCCCTGAG ATGGTCCGTC ACCCCGCGTT CGCCATTGTC 
TTCCAGCTGG AGTACGTGTT TAACAGCCCC TCGGGGGCAG ACAGCAATGC AGCTTCACTC 
TCCTCCTTGT CCAGCCTGGC ATGTATGCAC ATGGTTCGCT GGGCTGTCTG GAACCCCTCG 
CTGGAAGCCA GCTCCGGGCA GGTGATGTTA CCTCTCCAGG GTGGCATCCA GTCCAACCCT 
TCGCACTGTC TGGTCTATAA GGTACCCCCA GCCAGCATAA GCTCCGAAGA GGTGAAGCAG 
GTGGAGTCAG GGACGGTCCA GTTCCAGTAC TCGCTGAGCC CTGAAGAACA CCCAGGCACA 
CCCACAGCGA ATGCAAGCAG CCCCAAGGCA GAGCGGCGGT CTTTTAGGAA GCCACCCGTG 
TCCCCTTCAA GTAAGTGTGT GCTGAGAACT GAGTCTTCAA GCTCATGGCA CAGCTCCATG 
TGTGCCCAGG TCCACCAGCA TCGCCGTCCC AGGGGCTCAC CGCTGCCCAG GACTCACCCA 
TGGGACCAGG GCTGTCACTT TCTCAGCTGG CGGCTTCCCC ACCATCCCCA GCTCCCCGCC 
GCCGCTCGGC CAAACCTTCG TCACAGCCAC CTGGGAGCCC TGTGCCCTCC CTGGTCCCAG 
GGGAACCCAG ACCAGGAGTC GGCCTTCTTC TGGAGGCTGG CTCAGGGGGC CATCCGTTCC 
AGCTCTGGAG GGAAGCTGTC ACGGGCATCC CTGGCGCTGC TGCAGTCCGC TGGCTTTCCC 
GAGATTCTGG ACGCCAACAA GCAACCAGCT GAGGCTGTGA GTCCTACAGA CCCCGTGAAG 
TTCAGCCCTC AGAAGGAAGA ATCAGACTGT CTACAAGGCA ATGAGATAGT GTTGCAATTT 
CTTGCCTTTA GCAGGGTGCC CCAGGATGAG CAGGCAGCGC CATGGCCACA GACTGTGTAC 
TTCACCTTCC AATTCTACCG CTTCCCACCC GAGACCACGC CACGGCTGCA GCTCGTGGAG 
CTGGCCGATG CTGGGAAGAC CAGCTCTGCC TCCCTGTCGC ACATCCTCGC CCAGATCCAG 
AAGGATGGCT CCTTTGATGC GGGGTCTCCG GGCTTTCAGC GGAAGTACCT GGTGGACCCC 
GGGTTCCTGA GACCCGGGGA GCAGCGCTGG TTCGCCCGCT ACCTGGCCAT GCAGACCCTG 
CAGATCGACG TCTGGGATGG GGACTCCCTG CTTCTCATTG GATCTGCTGC CGTTCAGCTG 
AAGCACCTTC TCCGCCAGGG GCGGCCGGCC GTGCAGGTCT CGCATGAGCT GGAGGTTGTG 
GCGACTGAAT ACGAGCAGGA CGCCATGGTG GTGAGTGGAG ACATGACCAA GTTTGGCTGC 
GTCAGACCCA TCGACGTCCA CACGGTGGTG AAGGGCCAGC TGCACTTGAC CTTGGCCAAC 
GTGGGTCACT TGTGTGAACA GAGAGTAAGG AGTCCCAGTT CCCTGCCACC TTCCAGGTCG 
AGGGTCATCT CAAATGATGG AGTTGGCAAC TTCATGGGAG GCAGCCTCCT GACACCCGGG 
GGCCCCAGAT CAGTGAAAAA TGTGGTCCAA GCTCAGAAGC TGGCTGATGT GGACAGCGAA 
CTGGCCGCCC TGCTCTTCAC ACAACCGAGA CCAGGGGCCC GGGGGCCCCA GGGCATCAAT 
CAAGAGGGGG ATGCCGTCCG CAGGCGCAAG CTGGAGCGCA TGAGGGCCGT GCGGCTGCGG 
GAATGTGGAT TGGAGCCGGG CCGCCGCGGG CTGGGCGCGC TGGCCCAGCA GAGCCTCCAT 
GCCCAGCACG CCCGAGACCT GCAGGTCATT GCCGCCTACC GTGAGCGCAC AAAGGCGGAG 
AGCATCGCCA GCGCACTGAG CACGGCCATC ACCACGCAGC ACACCATCCA CGCCACCCTA 
GGCGCTGCCG AGTTCTTCGA GTTCGCCCTG AGGAACCCCC ACAACGCGCA GCACATGGTG 
ACCATCGAGA TAGACAACCC CGAGCTCAGT CTCATCCTGG ATGGTCAGGA GTGGCGGTAC 
TTCAAGGACA TGGCTGACTT GCACACACCC CTGGAGGAGG ACATGTTCCA CCTGCGGGGC 
AGCCTGGCCC CCCAACTCTT CCTGCGACCT CGGGAGACTG CCCACATCCC CTTCAAGTTC 
CAGACTTTCT CTGTGGGCCC CTGGGCCTCG GCACAGGCCT CTGCTGAGCT GAGATCAGAG 
AAGGACATGG ATGCCGGATC ACCCTATAAG TCCAGCATGA TGCCCACCAA ACACGCCAAG 
GTCCTGTTCC GCGCGGGCAG CGGCAAGCCC CTGGCCGTGC TCTGCCTGAC GGTGGAGCTG 
CAGCCTCACG TGGTGGACCA GGTCTTCCGC TTCCACCACC CGGAGCTCAC CTTCCTGAAG 
AAAGCCATCC GCCTGCCACC CTGGCACACG CTTCCAGGTG CCCCTGTAGG GACTTCCAGT 
GAGGACCCCC CTTTCCATGT TCGATGTAGT GACCCGAACA TCATTTGTGA GACACAGAGT 
GTGGGCCCCG GGGAGCCCCG TGACGTGTTC CTGAAGGTGG CCAGTGGCCC TAGCCCAGAG 
GTCAAGGACT TCTTTGTCAC CATCTACGTA GACCGCTGGT TAGCAACACC TGTCCAGACC 
TGGCAGGTCC ACCTGCACTC GCTGCAGCGA GTGGACGTCT CCTGTATCAC TGGCCAGAGG 
ACCCGCCTGG CTCTCGTCCT CCGGGGGACG CAGACAGTTA GGAGAGTGCA GGCTTTCAGC 
TCACACCCCC AGGAGCTGAC GACGGACCCC AGAGGTGTGT TCGTGCTGCC TCCTCACGGG 
GTGCAGGACT TGCATGTGAG CGTCAGGCCC TGCAGGCCGG GCAGCCGCTT CGCGCACCTC 
CACCTGGTTG ACGTGGACGC CCACCAGCTG GTGGCCTCGT GGCTCCTGTG TCTCTCCTGC 
CGGCCGCCTC TCATCTCCAA GGCCTTCGAG ATAAAGCTGC CCGCGGGGAG CGGGAGGGGC 
GCCAATAAGC GCATCACCTA CACCAATCCC TACCCCTCGC GGAGGGCCTA CCGCCTGCAC 
AGCGACCACC CCCACCTGCT GCAGTTCAAG GAGGACGCCT TCCAGGTCGG GGGAGGTGAG 
ACCTATACCA TCGGCCTGCG GTTTGTGTCC CGTCAGAGAG CCGGGGAGGA AGAGATCCTG 
ATTTACATCA ACGACCACGA AGACAAAAAC GAGGAGACCT TTTGCGTGAA GGTCATCTAC 
CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPHP4 ( XM_024976779.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832547.1 Bos taurus nephrocystin 4 (NPHP4), transcript variant X13, mRNA.

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CloneID	OBa223962
Clone ID Related Accession (Same CDS sequence)	XM_024976779.1
Accession Version	XM_024976779.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4101bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	nephrocystin-4 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACGACT GGCACAGGAT CTTTGACCAA AACCTGCTGG TCCCTCCGCA CCCACAGAGA 
GCACGCCAGC CTCTGAAGGA ATCGACGGCG TTTCAGTGTG TCCTGAAGTG GCTTGATGGC 
CCGCTAATTA AGCAGGGGGT GCTGGAGCTG CTATCGGAGG TGGGCTGCCA CCTGCGCGTG 
TCTCTCTTCG ATGTCACCTA TCGGCACTTC TTCGGGAGGA CGTGGAGGAG CTCGCCGAGG 
CCTGCAGAGC CGCTCACCAG GCAGCCGGCC AGGATCGTCT TCAATGAGCC CTTGTATTTT 
CACACGTCCT TGAACCACCC TCATATTGTG GCCGTGGTGG AGGTGGTCGT TGAAGGCAGA 
AGACGAGACG GGACCCTCCA GACTCTGTCC TGTGGGTTTG GAATTCTTCG GATCTTCAGC 
CACAAGCCGG AATCTCCAAC TTCCACTTCC CAGGACAAAC GGCTGAGACT GTATCATGGG 
AGCCCGCGAG CCCTCCTCCA CCCGCTCCTT CAGGACCCCA TAGAACAGAA CAAGCACATG 
ACCCTCATTG AGAACTGCAG CCTGCAGTAC ACGCTGAGGC CACACCCGCT CCTGGAGCCC 
GTGTTCCACC TTCTTCCCGA GAACTTGCTG GTGTCCGGTC TGCAGCAGAT ACCTGGCCTC 
CTTCCAGCTC ACGGGGAATC AGGAGATGCC CTCCGGAAGC CCCGCCTGCA GAAGCCTCTG 
ACGTGCTACC TGGATGATCT GTTCTTCACG CTGTATCCCT CGCTGGAGAA GTTCGAGGAG 
GAGCTTCTGG AACTCGTGAT CAGCGATTGC TTCCGGGAGG CGGGCAGCCC GCTGGACGTC 
GGCACCCTGG AGATCCTGGA GCGGCGCCTG TGCGTGGGCG TGCACAATGG GTTGGGCTTC 
GTGCAGCGGC CGCAGGTCCT CGTGCTGGTG CCCGAGCTGG AGGTGGCCTT GACACGCTCT 
GCCAGCTTCA GCCGGAAGGT CGGCTCCTCT TCCAAGGCCA GCTCTGGAAA CCAGGCGCTG 
GTCTTGAGAA GCCGTCTCCG ACTCCCTGAG ATGGTCCGTC ACCCCGCGTT CGCCATTGTC 
TTCCAGCTGG AGTACGTGTT TAACAGCCCC TCGGGGGCAG ACAGCAATGC AGCTTCACTC 
TCCTCCTTGT CCAGCCTGGC ATGTATGCAC ATGGTTCGCT GGGCTGTCTG GAACCCCTCG 
CTGGAAGCCA GCTCCGGGCA GGTGATGTTA CCTCTCCAGG GTGGCATCCA GTCCAACCCT 
TCGCACTGTC TGGTCTATAA GGTACCCCCA GCCAGCATAA GCTCCGAAGA GGTGAAGCAG 
GTGGAGTCAG GGACGGTCCA GTTCCAGTAC TCGCTGAGCC CTGAAGAACA CCCAGGCACA 
CCCACAGCGA ATGCAAGCAG CCCCAAGGCA GAGCGGCGGT CTTTTAGGAA GCCACCCGTG 
TCCCCTTCAA CTGTCACTTT CTCAGCTGGC GGCTTCCCCA CCATCCCCAG CTCCCCGCCG 
CCGCTCGGCC AAACCTTCGT CACAGCCACC TGGGAGCCCT GTGCCCTCCC TGGTCCCAGG 
GGAACCCAGA CCAGGAGCTC TGGAGGGAAG CTGTCACGGG CATCCCTGGC GCTGCTGCAG 
TCCGCTGGCT TTCCCGAGAT TCTGGACGCC AACAAGCAAC CAGCTGAGGC TGTGAGTCCT 
ACAGACCCCG TGAAGTTCAG CCCTCAGAAG GAAGAATCAG ACTGTCTACA AGGCAATGAG 
ATAGTGTTGC AATTTCTTGC CTTTAGCAGG GTGCCCCAGG ATGAGCAGGC AGCGCCATGG 
CCACAGACTG TGTACTTCAC CTTCCAATTC TACCGCTTCC CACCCGAGAC CACGCCACGG 
CTGCAGCTCG TGGAGCTGGC CGATGCTGGG AAGACCAGCT CTGCCTCCCT GTCGCACATC 
CTCGCCCAGA TCCAGAAGGA TGGCTCCTTT GATGCGGGGT CTCCGGGCTT TCAGCGGAAG 
TACCTGGTGG ACCCCGGGTT CCTGAGACCC GGGGAGCAGC GCTGGTTCGC CCGCTACCTG 
GCCATGCAGA CCCTGCAGAT CGACGTCTGG GATGGGGACT CCCTGCTTCT CATTGGATCT 
GCTGCCGTTC AGCTGAAGCA CCTTCTCCGC CAGGGGCGGC CGGCCGTGCA GGTCTCGCAT 
GAGCTGGAGG TTGTGGCGAC TGAATACGAG CAGGACGCCA TGGTGGTGAG TGGAGACATG 
ACCAAGTTTG GCTGCGTCAG ACCCATCGAC GTCCACACGG TGGTGAAGGG CCAGCTGCAC 
TTGACCTTGG CCAACGTGGG TCACTTGTGT GAACAGAGAG TAAGGAGTCC CAGTTCCCTG 
CCACCTTCCA GGTCGAGGGT CATCTCAAAT GATGGAGTTG GCAACTTCAT GGGAGGCAGC 
CTCCTGACAC CCGGGGGCCC CAGATCAGTG AAAAATGTGG TCCAAGCTCA GAAGCTGGCT 
GATGTGGACA GCGAACTGGC CGCCCTGCTC TTCACACAAC CGAGACCAGG GGCCCGGGGG 
CCCCAGGGCA TCAATCAAGA GGGGGATGCC GTCCGCAGGC GCAAGCTGGA GCGCATGAGG 
GCCGTGCGGC TGCGGGAATG TGGATTGGAG CCGGGCCGCC GCGGGCTGGG CGCGCTGGCC 
CAGCAGAGCC TCCATGCCCA GCACGCCCGA GACCTGCAGG TCATTGCCGC CTACCGTGAG 
CGCACAAAGG CGGAGAGCAT CGCCAGCGCA CTGAGCACGG CCATCACCAC GCAGCACACC 
ATCCACGCCA CCCTAGGCGC TGCCGAGTTC TTCGAGTTCG CCCTGAGGAA CCCCCACAAC 
GCGCAGCACA TGGTGACCAT CGAGATAGAC AACCCCGAGC TCAGTCTCAT CCTGGATGGT 
CAGGAGTGGC GGTACTTCAA GGACATGGCT GACTTGCACA CACCCCTGGA GGAGGACATG 
TTCCACCTGC GGGGCAGCCT GGCCCCCCAA CTCTTCCTGC GACCTCGGGA GACTGCCCAC 
ATCCCCTTCA AGTTCCAGAC TTTCTCTGTG GGCCCCTGGG CCTCGGCACA GGCCTCTGCT 
GAGCTGAGAT CAGAGAAGGA CATGGATGCC GGATCACCCT ATAAGTCCAG CATGATGCCC 
ACCAAACACG CCAAGGTCCT GTTCCGCGCG GGCAGCGGCA AGCCCCTGGC CGTGCTCTGC 
CTGACGGTGG AGCTGCAGCC TCACGTGGTG GACCAGGTCT TCCGCTTCCA CCACCCGGAG 
CTCACCTTCC TGAAGAAAGC CATCCGCCTG CCACCCTGGC ACACGCTTCC AGGTGCCCCT 
GTAGGGACTT CCAGTGAGGA CCCCCCTTTC CATGTTCGAT GTAGTGACCC GAACATCATT 
TGTGAGACAC AGAGTGTGGG CCCCGGGGAG CCCCGTGACG TGTTCCTGAA GGTGGCCAGT 
GGCCCTAGCC CAGAGGTCAA GGACTTCTTT GTCACCATCT ACGTAGACCG CTGGTTAGCA 
ACACCTGTCC AGACCTGGCA GGTCCACCTG CACTCGCTGC AGCGAGTGGA CGTCTCCTGT 
ATCACTGGCC AGAGGACCCG CCTGGCTCTC GTCCTCCGGG GGACGCAGAC AGTTAGGAGA 
GTGCAGGCTT TCAGCTCACA CCCCCAGGAG CTGACGACGG ACCCCAGAGG TGTGTTCGTG 
CTGCCTCCTC ACGGGGTGCA GGACTTGCAT GTGAGCGTCA GGCCCTGCAG GCCGGGCAGC 
CGCTTCGCGC ACCTCCACCT GGTTGACGTG GACGCCCACC AGCTGGTGGC CTCGTGGCTC 
CTGTGTCTCT CCTGCCGGCC GCCTCTCATC TCCAAGGCCT TCGAGATAAA GCTGCCCGCG 
GGGAGCGGGA GGGGCGCCAA TAAGCGCATC ACCTACACCA ATCCCTACCC CTCGCGGAGG 
GCCTACCGCC TGCACAGCGA CCACCCCCAC CTGCTGCAGT TCAAGGAGGA CGCCTTCCAG 
GTCGGGGGAG GTGAGACCTA TACCATCGGC CTGCGGTTTG TGTCCCGTCA GAGAGCCGGG 
GAGGAAGAGA TCCTGATTTA CATCAACGAC CACGAAGACA AAAACGAGGA GACCTTTTGC 
GTGAAGGTCA TCTACCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832547.1
CDS	281..4381
Translation	MHDWHRIFDQNLLVPPHPQRARQPLKESTAFQCVLKWLDGPLIKQGVLELLSEVGCHLRVSLFDVTYRHFFGRTWRSSPRPAEPLTRQPARIVFNEPLYFHTSLNHPHIVAVVEVVVEGRRRDGTLQTLSCGFGILRIFSHKPESPTSTSQDKRLRLYHGSPRALLHPLLQDPIEQNKHMTLIENCSLQYTLRPHPLLEPVFHLLPENLLVSGLQQIPGLLPAHGESGDALRKPRLQKPLTCYLDDLFFTLYPSLEKFEEELLELVISDCFREAGSPLDVGTLEILERRLCVGVHNGLGFVQRPQVLVLVPELEVALTRSASFSRKVGSSSKASSGNQALVLRSRLRLPEMVRHPAFAIVFQLEYVFNSPSGADSNAASLSSLSSLACMHMVRWAVWNPSLEASSGQVMLPLQGGIQSNPSHCLVYKVPPASISSEEVKQVESGTVQFQYSLSPEEHPGTPTANASSPKAERRSFRKPPVSPSTVTFSAGGFPTIPSSPPPLGQTFVTATWEPCALPGPRGTQTRSSGGKLSRASLALLQSAGFPEILDANKQPAEAVSPTDPVKFSPQKEESDCLQGNEIVLQFLAFSRVPQDEQAAPWPQTVYFTFQFYRFPPETTPRLQLVELADAGKTSSASLSHILAQIQKDGSFDAGSPGFQRKYLVDPGFLRPGEQRWFARYLAMQTLQIDVWDGDSLLLIGSAAVQLKHLLRQGRPAVQVSHELEVVATEYEQDAMVVSGDMTKFGCVRPIDVHTVVKGQLHLTLANVGHLCEQRVRSPSSLPPSRSRVISNDGVGNFMGGSLLTPGGPRSVKNVVQAQKLADVDSELAALLFTQPRPGARGPQGINQEGDAVRRRKLERMRAVRLRECGLEPGRRGLGALAQQSLHAQHARDLQVIAAYRERTKAESIASALSTAITTQHTIHATLGAAEFFEFALRNPHNAQHMVTIEIDNPELSLILDGQEWRYFKDMADLHTPLEEDMFHLRGSLAPQLFLRPRETAHIPFKFQTFSVGPWASAQASAELRSEKDMDAGSPYKSSMMPTKHAKVLFRAGSGKPLAVLCLTVELQPHVVDQVFRFHHPELTFLKKAIRLPPWHTLPGAPVGTSSEDPPFHVRCSDPNIICETQSVGPGEPRDVFLKVASGPSPEVKDFFVTIYVDRWLATPVQTWQVHLHSLQRVDVSCITGQRTRLALVLRGTQTVRRVQAFSSHPQELTTDPRGVFVLPPHGVQDLHVSVRPCRPGSRFAHLHLVDVDAHQLVASWLLCLSCRPPLISKAFEIKLPAGSGRGANKRITYTNPYPSRRAYRLHSDHPHLLQFKEDAFQVGGGETYTIGLRFVSRQRAGEEEILIYINDHEDKNEETFCVKVIYQ

Target ORF information:

RefSeq Version	XM_024976779.1
Organism	Bos taurus(cattle)
Definition	Bos taurus nephrocystin 4 (NPHP4), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976779.1

ORF Insert Sequence:

ATGCACGACT GGCACAGGAT CTTTGACCAA AACCTGCTGG TCCCTCCGCA CCCACAGAGA 
GCACGCCAGC CTCTGAAGGA ATCGACGGCG TTTCAGTGTG TCCTGAAGTG GCTTGATGGC 
CCGCTAATTA AGCAGGGGGT GCTGGAGCTG CTATCGGAGG TGGGCTGCCA CCTGCGCGTG 
TCTCTCTTCG ATGTCACCTA TCGGCACTTC TTCGGGAGGA CGTGGAGGAG CTCGCCGAGG 
CCTGCAGAGC CGCTCACCAG GCAGCCGGCC AGGATCGTCT TCAATGAGCC CTTGTATTTT 
CACACGTCCT TGAACCACCC TCATATTGTG GCCGTGGTGG AGGTGGTCGT TGAAGGCAGA 
AGACGAGACG GGACCCTCCA GACTCTGTCC TGTGGGTTTG GAATTCTTCG GATCTTCAGC 
CACAAGCCGG AATCTCCAAC TTCCACTTCC CAGGACAAAC GGCTGAGACT GTATCATGGG 
AGCCCGCGAG CCCTCCTCCA CCCGCTCCTT CAGGACCCCA TAGAACAGAA CAAGCACATG 
ACCCTCATTG AGAACTGCAG CCTGCAGTAC ACGCTGAGGC CACACCCGCT CCTGGAGCCC 
GTGTTCCACC TTCTTCCCGA GAACTTGCTG GTGTCCGGTC TGCAGCAGAT ACCTGGCCTC 
CTTCCAGCTC ACGGGGAATC AGGAGATGCC CTCCGGAAGC CCCGCCTGCA GAAGCCTCTG 
ACGTGCTACC TGGATGATCT GTTCTTCACG CTGTATCCCT CGCTGGAGAA GTTCGAGGAG 
GAGCTTCTGG AACTCGTGAT CAGCGATTGC TTCCGGGAGG CGGGCAGCCC GCTGGACGTC 
GGCACCCTGG AGATCCTGGA GCGGCGCCTG TGCGTGGGCG TGCACAATGG GTTGGGCTTC 
GTGCAGCGGC CGCAGGTCCT CGTGCTGGTG CCCGAGCTGG AGGTGGCCTT GACACGCTCT 
GCCAGCTTCA GCCGGAAGGT CGGCTCCTCT TCCAAGGCCA GCTCTGGAAA CCAGGCGCTG 
GTCTTGAGAA GCCGTCTCCG ACTCCCTGAG ATGGTCCGTC ACCCCGCGTT CGCCATTGTC 
TTCCAGCTGG AGTACGTGTT TAACAGCCCC TCGGGGGCAG ACAGCAATGC AGCTTCACTC 
TCCTCCTTGT CCAGCCTGGC ATGTATGCAC ATGGTTCGCT GGGCTGTCTG GAACCCCTCG 
CTGGAAGCCA GCTCCGGGCA GGTGATGTTA CCTCTCCAGG GTGGCATCCA GTCCAACCCT 
TCGCACTGTC TGGTCTATAA GGTACCCCCA GCCAGCATAA GCTCCGAAGA GGTGAAGCAG 
GTGGAGTCAG GGACGGTCCA GTTCCAGTAC TCGCTGAGCC CTGAAGAACA CCCAGGCACA 
CCCACAGCGA ATGCAAGCAG CCCCAAGGCA GAGCGGCGGT CTTTTAGGAA GCCACCCGTG 
TCCCCTTCAA CTGTCACTTT CTCAGCTGGC GGCTTCCCCA CCATCCCCAG CTCCCCGCCG 
CCGCTCGGCC AAACCTTCGT CACAGCCACC TGGGAGCCCT GTGCCCTCCC TGGTCCCAGG 
GGAACCCAGA CCAGGAGCTC TGGAGGGAAG CTGTCACGGG CATCCCTGGC GCTGCTGCAG 
TCCGCTGGCT TTCCCGAGAT TCTGGACGCC AACAAGCAAC CAGCTGAGGC TGTGAGTCCT 
ACAGACCCCG TGAAGTTCAG CCCTCAGAAG GAAGAATCAG ACTGTCTACA AGGCAATGAG 
ATAGTGTTGC AATTTCTTGC CTTTAGCAGG GTGCCCCAGG ATGAGCAGGC AGCGCCATGG 
CCACAGACTG TGTACTTCAC CTTCCAATTC TACCGCTTCC CACCCGAGAC CACGCCACGG 
CTGCAGCTCG TGGAGCTGGC CGATGCTGGG AAGACCAGCT CTGCCTCCCT GTCGCACATC 
CTCGCCCAGA TCCAGAAGGA TGGCTCCTTT GATGCGGGGT CTCCGGGCTT TCAGCGGAAG 
TACCTGGTGG ACCCCGGGTT CCTGAGACCC GGGGAGCAGC GCTGGTTCGC CCGCTACCTG 
GCCATGCAGA CCCTGCAGAT CGACGTCTGG GATGGGGACT CCCTGCTTCT CATTGGATCT 
GCTGCCGTTC AGCTGAAGCA CCTTCTCCGC CAGGGGCGGC CGGCCGTGCA GGTCTCGCAT 
GAGCTGGAGG TTGTGGCGAC TGAATACGAG CAGGACGCCA TGGTGGTGAG TGGAGACATG 
ACCAAGTTTG GCTGCGTCAG ACCCATCGAC GTCCACACGG TGGTGAAGGG CCAGCTGCAC 
TTGACCTTGG CCAACGTGGG TCACTTGTGT GAACAGAGAG TAAGGAGTCC CAGTTCCCTG 
CCACCTTCCA GGTCGAGGGT CATCTCAAAT GATGGAGTTG GCAACTTCAT GGGAGGCAGC 
CTCCTGACAC CCGGGGGCCC CAGATCAGTG AAAAATGTGG TCCAAGCTCA GAAGCTGGCT 
GATGTGGACA GCGAACTGGC CGCCCTGCTC TTCACACAAC CGAGACCAGG GGCCCGGGGG 
CCCCAGGGCA TCAATCAAGA GGGGGATGCC GTCCGCAGGC GCAAGCTGGA GCGCATGAGG 
GCCGTGCGGC TGCGGGAATG TGGATTGGAG CCGGGCCGCC GCGGGCTGGG CGCGCTGGCC 
CAGCAGAGCC TCCATGCCCA GCACGCCCGA GACCTGCAGG TCATTGCCGC CTACCGTGAG 
CGCACAAAGG CGGAGAGCAT CGCCAGCGCA CTGAGCACGG CCATCACCAC GCAGCACACC 
ATCCACGCCA CCCTAGGCGC TGCCGAGTTC TTCGAGTTCG CCCTGAGGAA CCCCCACAAC 
GCGCAGCACA TGGTGACCAT CGAGATAGAC AACCCCGAGC TCAGTCTCAT CCTGGATGGT 
CAGGAGTGGC GGTACTTCAA GGACATGGCT GACTTGCACA CACCCCTGGA GGAGGACATG 
TTCCACCTGC GGGGCAGCCT GGCCCCCCAA CTCTTCCTGC GACCTCGGGA GACTGCCCAC 
ATCCCCTTCA AGTTCCAGAC TTTCTCTGTG GGCCCCTGGG CCTCGGCACA GGCCTCTGCT 
GAGCTGAGAT CAGAGAAGGA CATGGATGCC GGATCACCCT ATAAGTCCAG CATGATGCCC 
ACCAAACACG CCAAGGTCCT GTTCCGCGCG GGCAGCGGCA AGCCCCTGGC CGTGCTCTGC 
CTGACGGTGG AGCTGCAGCC TCACGTGGTG GACCAGGTCT TCCGCTTCCA CCACCCGGAG 
CTCACCTTCC TGAAGAAAGC CATCCGCCTG CCACCCTGGC ACACGCTTCC AGGTGCCCCT 
GTAGGGACTT CCAGTGAGGA CCCCCCTTTC CATGTTCGAT GTAGTGACCC GAACATCATT 
TGTGAGACAC AGAGTGTGGG CCCCGGGGAG CCCCGTGACG TGTTCCTGAA GGTGGCCAGT 
GGCCCTAGCC CAGAGGTCAA GGACTTCTTT GTCACCATCT ACGTAGACCG CTGGTTAGCA 
ACACCTGTCC AGACCTGGCA GGTCCACCTG CACTCGCTGC AGCGAGTGGA CGTCTCCTGT 
ATCACTGGCC AGAGGACCCG CCTGGCTCTC GTCCTCCGGG GGACGCAGAC AGTTAGGAGA 
GTGCAGGCTT TCAGCTCACA CCCCCAGGAG CTGACGACGG ACCCCAGAGG TGTGTTCGTG 
CTGCCTCCTC ACGGGGTGCA GGACTTGCAT GTGAGCGTCA GGCCCTGCAG GCCGGGCAGC 
CGCTTCGCGC ACCTCCACCT GGTTGACGTG GACGCCCACC AGCTGGTGGC CTCGTGGCTC 
CTGTGTCTCT CCTGCCGGCC GCCTCTCATC TCCAAGGCCT TCGAGATAAA GCTGCCCGCG 
GGGAGCGGGA GGGGCGCCAA TAAGCGCATC ACCTACACCA ATCCCTACCC CTCGCGGAGG 
GCCTACCGCC TGCACAGCGA CCACCCCCAC CTGCTGCAGT TCAAGGAGGA CGCCTTCCAG 
GTCGGGGGAG GTGAGACCTA TACCATCGGC CTGCGGTTTG TGTCCCGTCA GAGAGCCGGG 
GAGGAAGAGA TCCTGATTTA CATCAACGAC CACGAAGACA AAAACGAGGA GACCTTTTGC 
GTGAAGGTCA TCTACCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPHP4 ( XM_015466839.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015322325.1 Bos taurus nephronophthisis 4 (NPHP4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa92992
Clone ID Related Accession (Same CDS sequence)	XM_015466839.1 , XM_015475178.1
Accession Version	XM_015466839.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4530bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	nephrocystin-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646324.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGTGC CGTCGCCGCC AGTTCCAAGC CGGGTCTCCA TCACAGCTCC CTGCACCCTC 
CTCTCCTCTG TGATTTTCAT AGGTCACAAC CACCTCTTTT GTGTGCTAAC TTGTATCCTG 
TGTTTCTGCC TCCCTTCAGA TACATTCCTT GAGGTCATCG TGGTTGTCTG CAGTGTTCTC 
CAGGGCCTGG CACAGGGTAA TCCCAGAGGC CCCAGGGACC CGGGGACCAG CAGAATGCAC 
GACTGGCACA GGATCTTTGA CCAAAACCTG CTGGTCCCTC CGCACCCACA GAGAGCACGC 
CAGCCTCTGA AGGAATCGAC GGCGTTTCAG TGTGTCCTGA AGTGGCTTGA TGGCCCGCTA 
ATTAAGCAGG GGGTGCTGGA GCTGCTATCG GAGGTGGGCT GCCACCTGCG CGTGTCTCTC 
TTCGATGTCA CCTATCGGCA CTTCTTCGGG AGGACGTGGA GGAGCTCGCC GAGGCCTGCA 
GAGCCGCTCA CCAGGCAGCC GGCCAGGATC GTCTTCAATG AGCCCTTGTA TTTTCACACG 
TCCTTGAACC ACCCTCATAT TGTGGCCGTG GTGGAGGTGG TCGTTGAAGG CAGAAGACGA 
GACGGGACCC TCCAGACTCT GTCCTGTGGG TTTGGAATTC TTCGGATCTT CAGCCACAAG 
CCGGAATCTC CAACTTCCAC TTCCCAGGAC AAACGGCTGA GACTGTATCA TGGGAGCCCG 
CGAGCCCTCC TCCACCCGCT CCTTCAGGAC CCCATAGAAC AGAACAAGCA CATGACCCTC 
ATTGAGAACT GCAGCCTGCA GTACACGCTG AGGCCACACC CGCTCCTGGA GCCCGTGTTC 
CACCTTCTTC CCGAGAACTT GCTGGTGTCC GGTCTGCAGC AGATACCTGG CCTCCTTCCA 
GCTCACGGGG AATCAGGAGA TGCCCTCCGG AAGCCCCGCC TGCAGAAGCC TCTGACGTGC 
TACCTGGATG ATCTGTTCTT CACGCTGTAT CCCTCGCTGG AGAAGTTCGA GGAGGAGCTT 
CTGGAACTCG TGATCAGCGA TTGCTTCCGG GAGGCGGGCA GCCCGCTGGA CGTCGGCACC 
CTGGAGATCC TGGAGCGGCG CCTGTGCGTG GGCGTGCACA ATGGGTTGGG CTTCGTGCAG 
CGGCCGCAGG TCCTCGTGCT GGTGCCCGAG CTGGAGGTGG CCTTGACACG CTCTGCCAGC 
TTCAGCCGGA AGGTCGGCTC CTCTTCCAAG GCCAGCTCTG GAAACCAGGC GCTGGTCTTG 
AGAAGCCGTC TCCGACTCCC TGAGATGGTC CGTCACCCCG CGTTCGCCAT TGTCTTCCAG 
CTGGAGTACG TGTTTAACAG CCCCTCGGGG GCAGACAGCA ATGCAGCTTC ACTCTCCTCC 
TTGTCCAGCC TGGCATGTAT GCACATGGTT CGCTGGGCTG TCTGGAACCC CTCGCTGGAA 
GCCAGCTCCG GGCAGGTGAT GTTACCTCTC CAGGGTGGCA TCCAGTCCAA CCCTTCGCAC 
TGTCTGGTCT ATAAGGTACC CCCAGCCAGC ATAAGCTCCG AAGAGGTGAA GCAGGTGGAG 
TCAGGGACGG TCCAGTTCCA GTACTCGCTG AGCCCTGAAG AACACCCAGG CACACCCACA 
GCGAATGCAA GCAGCCCCAA GGCAGAGCGG CGGTCTTTTA GGAAGCCACC CGTGTCCCCT 
TCAAGTCCAC CAGCATCGCC GTCCCAGGGG CTCACCGCTG CCCAGGACTC ACCCATGGGA 
CCAGGGCTGT CACTTTCTCA GCTGGCGGCT TCCCCACCAT CCCCAGCTCC CCGCCGCCGC 
TCGGCCAAAC CTTCGTCACA GCCACCTGGG AGCCCTGTGC CCTCCCTGGT CCCAGGGGAG 
TTCCCCTTGG AGGGCAGGAT CTCTCACCTG GAGGCTGACC TGAGCCAGAC AGCCTTAGCC 
CTGGGAACGT CCGCTGCTGA CCACTTGCAG GAACTGCCTT TCACACCCGT GCACGCCCCG 
ATCATTGCAG GAACCCAGAC CAGGAGCTCT GGAGGGAAGC TGTCACGGGC ATCCCTGGCG 
CTGCTGCAGT CCGCTGGCTT TCCCGAGATT CTGGACGCCA ACAAGCAACC AGCTGAGGCT 
GTGAGTCCTA CAGACCCCGT GAAGTTCAGC CCTCAGAAGG AAGAATCAGA CTGTCTACAA 
GGCAATGAGA TAGTGTTGCA ATTTCTTGCC TTTAGCAGGG TGCCCCAGGA TGAGCAGGCA 
GCGCCATGGC CACAGACTGT GTACTTCACC TTCCAATTCT ACCGCTTCCC ACCCGAGACC 
ACGCCACGGC TGCAGCTCGT GGAGCTGGCC GATGCTGGGA AGACCAGCTC TGCCTCCCTG 
TCGCACATCC TCGCCCAGAT CCAGAAGGAT GGCTCCTTTG ATGCGGGGTC TCCGGGCTTT 
CAGCGGAAGT ACCTGGTGGA CCCCGGGTTC CTGAGACCCG GGGAGCAGCG CTGGTTCGCC 
CGCTACCTGG CCATGCAGAC CCTGCAGATC GACGTCTGGG ATGGGGACTC CCTGCTTCTC 
ATTGGATCTG CTGCCGTTCA GCTGAAGCAC CTTCTCCGCC AGGGGCGGCC GGCCGTGCAG 
GTCTCGCATG AGCTGGAGGT TGTGGCGACT GAATACGAGC AGGACGCCAT GGTGGTGAGT 
GGAGACATGA CCAAGTTTGG CTGCGTCAGA CCCATCGACG TCCACACGGT GGTGAAGGGC 
CAGCTGCACT TGACCTTGGC CAACGTGGGT CACTTGTGTG AACAGAGAGT AAGGAGTCCC 
AGTTCCCTGC CACCTTCCAG GTCGAGGGTC ATCTCAAATG ATGGAGTTGG CAACTTCATG 
GGAGGCAGCC TCCTGACACC CGGGGGCCCC AGATCAGTGA AAAATGTGGT CCAAGCTCAG 
AAGCTGGCTG ATGTGGACAG CGAACTGGCC GCCCTGCTCT TCACACAACC GAGACCAGGG 
GCCCGGGGGC CCCAGGGCAT CAATCAAGAG GGGGATGCCG TCCGCAGGCG CAAGCTGGAG 
CGCATGAGGG CCGTGCGGCT GCGGGAATGT GGATTGGAGC CGGGCCGCCG CGGGCTGGGC 
GCGCTGGCCC AGCAGAGCCT CCATGCCCAG CACGCCCGAG ACCTGCAGGT CATTGCCGCC 
TACCGTGAGC GCACAAAGGC GGAGAGCATC GCCAGCGCAC TGAGCACGGC CATCACCACG 
CAGCACACCA TCCACGCCAC CCTAGGCGCT GCCGAGTTCT TCGAGTTCGC CCTGAGGAAC 
CCCCACAACG CGCAGCACAT GGTGACCATC GAGATAGACA ACCCCGAGCT CAGTCTCATC 
CTGGATGGTC AGGAGTGGCG GTACTTCAAG GACATGGCTG ACTTGCACAC ACCCCTGGAG 
GAGGACATGT TCCACCTGCG GGGCAGCCTG GCCCCCCAAC TCTTCCTGCG ACCTCGGGAG 
ACTGCCCACA TCCCCTTCAA GTTCCAGACT TTCTCTGTGG GCCCCTGGGC CTCGGCACAG 
GCCTCTGCTG AGCTGAGATC AGAGAAGGAC ATGGATGCCG GATCACCCTA TAAGTCCAGC 
ATGATGCCCA CCAAACACGC CAAGGTCCTG TTCCGCGCGG GCAGCGGCAA GCCCCTGGCC 
GTGCTCTGCC TGACGGTGGA GCTGCAGCCT CACGTGGTGG ACCAGGTCTT CCGCTTCCAC 
CACCCGGAGC TCACCTTCCT GAAGAAAGCC ATCCGCCTGC CACCCTGGCA CACGCTTCCA 
GGTGCCCCTG TAGGGACTTC CAGTGAGGAC CCCCCTTTCC ATGTTCGATG TAGTGACCCG 
AACATCATTT GTGAGACACA GAGTGTGGGC CCCGGGGAGC CCCGTGACGT GTTCCTGAAG 
GTGGCCAGTG GCCCTAGCCC AGAGGTCAAG GACTTCTTTG TCACCATCTA CGTAGACCGC 
TGGTTAGCAA CACCTGTCCA GACCTGGCAG GTCCACCTGC ACTCGCTGCA GCGAGTGGAC 
GTCTCCTGTA TCACTGGCCA GAGGACCCGC CTGGCTCTCG TCCTCCGGGG GACGCAGACA 
GTTAGGAGAG TGCAGGCTTT CAGCTCACAC CCCCAGGAGC TGACGACGGA CCCCAGAGGT 
GTGTTCGTGC TGCCTCCTCA CGGGGTGCAG GACTTGCATG TGAGCGTCAG GCCCTGCAGG 
CCGGGCAGCC GCTTCGCGCA CCTCCACCTG GTTGACGTGG ACGCCCACCA GCTGGTGGCC 
TCGTGGCTCC TGTGTCTCTC CTGCCGGCCG CCTCTCATCT CCAAGGCCTT CGAGATAAAG 
CTGCCCGCGG GGAGCGGGAG GGGCGCCAAT AAGCGCATCA CCTACACCAA TCCCTACCCC 
TCGCGGAGGG CCTACCGCCT GCACAGCGAC CACCCCCACC TGCTGCAGTT CAAGGAGGAC 
GCCTTCCAGG TCGGGGGAGG TGAGACCTAT ACCATCGGCC TGCGGTTTGT GTCCCGTCAG 
AGAGCCGGGG AGGAAGAGAT CCTGATTTAC ATCAACGACC ACGAAGACAA AAACGAGGAG 
ACCTTTTGCG TGAAGGTCAT CTACCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015322325.1
CDS	581..5110
Translation	MIVPSPPVPSRVSITAPCTLLSSVIFIGHNHLFCVLTCILCFCLPSDTFLEVIVVVCSVLQGLAQGNPRGPRDPGTSRMHDWHRIFDQNLLVPPHPQRARQPLKESTAFQCVLKWLDGPLIKQGVLELLSEVGCHLRVSLFDVTYRHFFGRTWRSSPRPAEPLTRQPARIVFNEPLYFHTSLNHPHIVAVVEVVVEGRRRDGTLQTLSCGFGILRIFSHKPESPTSTSQDKRLRLYHGSPRALLHPLLQDPIEQNKHMTLIENCSLQYTLRPHPLLEPVFHLLPENLLVSGLQQIPGLLPAHGESGDALRKPRLQKPLTCYLDDLFFTLYPSLEKFEEELLELVISDCFREAGSPLDVGTLEILERRLCVGVHNGLGFVQRPQVLVLVPELEVALTRSASFSRKVGSSSKASSGNQALVLRSRLRLPEMVRHPAFAIVFQLEYVFNSPSGADSNAASLSSLSSLACMHMVRWAVWNPSLEASSGQVMLPLQGGIQSNPSHCLVYKVPPASISSEEVKQVESGTVQFQYSLSPEEHPGTPTANASSPKAERRSFRKPPVSPSSPPASPSQGLTAAQDSPMGPGLSLSQLAASPPSPAPRRRSAKPSSQPPGSPVPSLVPGEFPLEGRISHLEADLSQTALALGTSAADHLQELPFTPVHAPIIAGTQTRSSGGKLSRASLALLQSAGFPEILDANKQPAEAVSPTDPVKFSPQKEESDCLQGNEIVLQFLAFSRVPQDEQAAPWPQTVYFTFQFYRFPPETTPRLQLVELADAGKTSSASLSHILAQIQKDGSFDAGSPGFQRKYLVDPGFLRPGEQRWFARYLAMQTLQIDVWDGDSLLLIGSAAVQLKHLLRQGRPAVQVSHELEVVATEYEQDAMVVSGDMTKFGCVRPIDVHTVVKGQLHLTLANVGHLCEQRVRSPSSLPPSRSRVISNDGVGNFMGGSLLTPGGPRSVKNVVQAQKLADVDSELAALLFTQPRPGARGPQGINQEGDAVRRRKLERMRAVRLRECGLEPGRRGLGALAQQSLHAQHARDLQVIAAYRERTKAESIASALSTAITTQHTIHATLGAAEFFEFALRNPHNAQHMVTIEIDNPELSLILDGQEWRYFKDMADLHTPLEEDMFHLRGSLAPQLFLRPRETAHIPFKFQTFSVGPWASAQASAELRSEKDMDAGSPYKSSMMPTKHAKVLFRAGSGKPLAVLCLTVELQPHVVDQVFRFHHPELTFLKKAIRLPPWHTLPGAPVGTSSEDPPFHVRCSDPNIICETQSVGPGEPRDVFLKVASGPSPEVKDFFVTIYVDRWLATPVQTWQVHLHSLQRVDVSCITGQRTRLALVLRGTQTVRRVQAFSSHPQELTTDPRGVFVLPPHGVQDLHVSVRPCRPGSRFAHLHLVDVDAHQLVASWLLCLSCRPPLISKAFEIKLPAGSGRGANKRITYTNPYPSRRAYRLHSDHPHLLQFKEDAFQVGGGETYTIGLRFVSRQRAGEEEILIYINDHEDKNEETFCVKVIYQ

Target ORF information:

RefSeq Version	XM_015466839.1
Organism	Bos taurus(cattle)
Definition	Bos taurus nephronophthisis 4 (NPHP4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015466839.1

ORF Insert Sequence:

ATGATTGTGC CGTCGCCGCC AGTTCCAAGC CGGGTCTCCA TCACAGCTCC CTGCACCCTC 
CTCTCCTCTG TGATTTTCAT AGGTCACAAC CACCTCTTTT GTGTGCTAAC TTGTATCCTG 
TGTTTCTGCC TCCCTTCAGA TACATTCCTT GAGGTCATCG TGGTTGTCTG CAGTGTTCTC 
CAGGGCCTGG CACAGGGTAA TCCCAGAGGC CCCAGGGACC CGGGGACCAG CAGAATGCAC 
GACTGGCACA GGATCTTTGA CCAAAACCTG CTGGTCCCTC CGCACCCACA GAGAGCACGC 
CAGCCTCTGA AGGAATCGAC GGCGTTTCAG TGTGTCCTGA AGTGGCTTGA TGGCCCGCTA 
ATTAAGCAGG GGGTGCTGGA GCTGCTATCG GAGGTGGGCT GCCACCTGCG CGTGTCTCTC 
TTCGATGTCA CCTATCGGCA CTTCTTCGGG AGGACGTGGA GGAGCTCGCC GAGGCCTGCA 
GAGCCGCTCA CCAGGCAGCC GGCCAGGATC GTCTTCAATG AGCCCTTGTA TTTTCACACG 
TCCTTGAACC ACCCTCATAT TGTGGCCGTG GTGGAGGTGG TCGTTGAAGG CAGAAGACGA 
GACGGGACCC TCCAGACTCT GTCCTGTGGG TTTGGAATTC TTCGGATCTT CAGCCACAAG 
CCGGAATCTC CAACTTCCAC TTCCCAGGAC AAACGGCTGA GACTGTATCA TGGGAGCCCG 
CGAGCCCTCC TCCACCCGCT CCTTCAGGAC CCCATAGAAC AGAACAAGCA CATGACCCTC 
ATTGAGAACT GCAGCCTGCA GTACACGCTG AGGCCACACC CGCTCCTGGA GCCCGTGTTC 
CACCTTCTTC CCGAGAACTT GCTGGTGTCC GGTCTGCAGC AGATACCTGG CCTCCTTCCA 
GCTCACGGGG AATCAGGAGA TGCCCTCCGG AAGCCCCGCC TGCAGAAGCC TCTGACGTGC 
TACCTGGATG ATCTGTTCTT CACGCTGTAT CCCTCGCTGG AGAAGTTCGA GGAGGAGCTT 
CTGGAACTCG TGATCAGCGA TTGCTTCCGG GAGGCGGGCA GCCCGCTGGA CGTCGGCACC 
CTGGAGATCC TGGAGCGGCG CCTGTGCGTG GGCGTGCACA ATGGGTTGGG CTTCGTGCAG 
CGGCCGCAGG TCCTCGTGCT GGTGCCCGAG CTGGAGGTGG CCTTGACACG CTCTGCCAGC 
TTCAGCCGGA AGGTCGGCTC CTCTTCCAAG GCCAGCTCTG GAAACCAGGC GCTGGTCTTG 
AGAAGCCGTC TCCGACTCCC TGAGATGGTC CGTCACCCCG CGTTCGCCAT TGTCTTCCAG 
CTGGAGTACG TGTTTAACAG CCCCTCGGGG GCAGACAGCA ATGCAGCTTC ACTCTCCTCC 
TTGTCCAGCC TGGCATGTAT GCACATGGTT CGCTGGGCTG TCTGGAACCC CTCGCTGGAA 
GCCAGCTCCG GGCAGGTGAT GTTACCTCTC CAGGGTGGCA TCCAGTCCAA CCCTTCGCAC 
TGTCTGGTCT ATAAGGTACC CCCAGCCAGC ATAAGCTCCG AAGAGGTGAA GCAGGTGGAG 
TCAGGGACGG TCCAGTTCCA GTACTCGCTG AGCCCTGAAG AACACCCAGG CACACCCACA 
GCGAATGCAA GCAGCCCCAA GGCAGAGCGG CGGTCTTTTA GGAAGCCACC CGTGTCCCCT 
TCAAGTCCAC CAGCATCGCC GTCCCAGGGG CTCACCGCTG CCCAGGACTC ACCCATGGGA 
CCAGGGCTGT CACTTTCTCA GCTGGCGGCT TCCCCACCAT CCCCAGCTCC CCGCCGCCGC 
TCGGCCAAAC CTTCGTCACA GCCACCTGGG AGCCCTGTGC CCTCCCTGGT CCCAGGGGAG 
TTCCCCTTGG AGGGCAGGAT CTCTCACCTG GAGGCTGACC TGAGCCAGAC AGCCTTAGCC 
CTGGGAACGT CCGCTGCTGA CCACTTGCAG GAACTGCCTT TCACACCCGT GCACGCCCCG 
ATCATTGCAG GAACCCAGAC CAGGAGCTCT GGAGGGAAGC TGTCACGGGC ATCCCTGGCG 
CTGCTGCAGT CCGCTGGCTT TCCCGAGATT CTGGACGCCA ACAAGCAACC AGCTGAGGCT 
GTGAGTCCTA CAGACCCCGT GAAGTTCAGC CCTCAGAAGG AAGAATCAGA CTGTCTACAA 
GGCAATGAGA TAGTGTTGCA ATTTCTTGCC TTTAGCAGGG TGCCCCAGGA TGAGCAGGCA 
GCGCCATGGC CACAGACTGT GTACTTCACC TTCCAATTCT ACCGCTTCCC ACCCGAGACC 
ACGCCACGGC TGCAGCTCGT GGAGCTGGCC GATGCTGGGA AGACCAGCTC TGCCTCCCTG 
TCGCACATCC TCGCCCAGAT CCAGAAGGAT GGCTCCTTTG ATGCGGGGTC TCCGGGCTTT 
CAGCGGAAGT ACCTGGTGGA CCCCGGGTTC CTGAGACCCG GGGAGCAGCG CTGGTTCGCC 
CGCTACCTGG CCATGCAGAC CCTGCAGATC GACGTCTGGG ATGGGGACTC CCTGCTTCTC 
ATTGGATCTG CTGCCGTTCA GCTGAAGCAC CTTCTCCGCC AGGGGCGGCC GGCCGTGCAG 
GTCTCGCATG AGCTGGAGGT TGTGGCGACT GAATACGAGC AGGACGCCAT GGTGGTGAGT 
GGAGACATGA CCAAGTTTGG CTGCGTCAGA CCCATCGACG TCCACACGGT GGTGAAGGGC 
CAGCTGCACT TGACCTTGGC CAACGTGGGT CACTTGTGTG AACAGAGAGT AAGGAGTCCC 
AGTTCCCTGC CACCTTCCAG GTCGAGGGTC ATCTCAAATG ATGGAGTTGG CAACTTCATG 
GGAGGCAGCC TCCTGACACC CGGGGGCCCC AGATCAGTGA AAAATGTGGT CCAAGCTCAG 
AAGCTGGCTG ATGTGGACAG CGAACTGGCC GCCCTGCTCT TCACACAACC GAGACCAGGG 
GCCCGGGGGC CCCAGGGCAT CAATCAAGAG GGGGATGCCG TCCGCAGGCG CAAGCTGGAG 
CGCATGAGGG CCGTGCGGCT GCGGGAATGT GGATTGGAGC CGGGCCGCCG CGGGCTGGGC 
GCGCTGGCCC AGCAGAGCCT CCATGCCCAG CACGCCCGAG ACCTGCAGGT CATTGCCGCC 
TACCGTGAGC GCACAAAGGC GGAGAGCATC GCCAGCGCAC TGAGCACGGC CATCACCACG 
CAGCACACCA TCCACGCCAC CCTAGGCGCT GCCGAGTTCT TCGAGTTCGC CCTGAGGAAC 
CCCCACAACG CGCAGCACAT GGTGACCATC GAGATAGACA ACCCCGAGCT CAGTCTCATC 
CTGGATGGTC AGGAGTGGCG GTACTTCAAG GACATGGCTG ACTTGCACAC ACCCCTGGAG 
GAGGACATGT TCCACCTGCG GGGCAGCCTG GCCCCCCAAC TCTTCCTGCG ACCTCGGGAG 
ACTGCCCACA TCCCCTTCAA GTTCCAGACT TTCTCTGTGG GCCCCTGGGC CTCGGCACAG 
GCCTCTGCTG AGCTGAGATC AGAGAAGGAC ATGGATGCCG GATCACCCTA TAAGTCCAGC 
ATGATGCCCA CCAAACACGC CAAGGTCCTG TTCCGCGCGG GCAGCGGCAA GCCCCTGGCC 
GTGCTCTGCC TGACGGTGGA GCTGCAGCCT CACGTGGTGG ACCAGGTCTT CCGCTTCCAC 
CACCCGGAGC TCACCTTCCT GAAGAAAGCC ATCCGCCTGC CACCCTGGCA CACGCTTCCA 
GGTGCCCCTG TAGGGACTTC CAGTGAGGAC CCCCCTTTCC ATGTTCGATG TAGTGACCCG 
AACATCATTT GTGAGACACA GAGTGTGGGC CCCGGGGAGC CCCGTGACGT GTTCCTGAAG 
GTGGCCAGTG GCCCTAGCCC AGAGGTCAAG GACTTCTTTG TCACCATCTA CGTAGACCGC 
TGGTTAGCAA CACCTGTCCA GACCTGGCAG GTCCACCTGC ACTCGCTGCA GCGAGTGGAC 
GTCTCCTGTA TCACTGGCCA GAGGACCCGC CTGGCTCTCG TCCTCCGGGG GACGCAGACA 
GTTAGGAGAG TGCAGGCTTT CAGCTCACAC CCCCAGGAGC TGACGACGGA CCCCAGAGGT 
GTGTTCGTGC TGCCTCCTCA CGGGGTGCAG GACTTGCATG TGAGCGTCAG GCCCTGCAGG 
CCGGGCAGCC GCTTCGCGCA CCTCCACCTG GTTGACGTGG ACGCCCACCA GCTGGTGGCC 
TCGTGGCTCC TGTGTCTCTC CTGCCGGCCG CCTCTCATCT CCAAGGCCTT CGAGATAAAG 
CTGCCCGCGG GGAGCGGGAG GGGCGCCAAT AAGCGCATCA CCTACACCAA TCCCTACCCC 
TCGCGGAGGG CCTACCGCCT GCACAGCGAC CACCCCCACC TGCTGCAGTT CAAGGAGGAC 
GCCTTCCAGG TCGGGGGAGG TGAGACCTAT ACCATCGGCC TGCGGTTTGT GTCCCGTCAG 
AGAGCCGGGG AGGAAGAGAT CCTGATTTAC ATCAACGACC ACGAAGACAA AAACGAGGAG 
ACCTTTTGCG TGAAGGTCAT CTACCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPHP4 ( XM_015475178.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015330664.1 Bos taurus nephronophthisis 4 (NPHP4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa92992
Clone ID Related Accession (Same CDS sequence)	XM_015466839.1 , XM_015475178.1
Accession Version	XM_015475178.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4530bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	nephrocystin-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104439.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGTGC CGTCGCCGCC AGTTCCAAGC CGGGTCTCCA TCACAGCTCC CTGCACCCTC 
CTCTCCTCTG TGATTTTCAT AGGTCACAAC CACCTCTTTT GTGTGCTAAC TTGTATCCTG 
TGTTTCTGCC TCCCTTCAGA TACATTCCTT GAGGTCATCG TGGTTGTCTG CAGTGTTCTC 
CAGGGCCTGG CACAGGGTAA TCCCAGAGGC CCCAGGGACC CGGGGACCAG CAGAATGCAC 
GACTGGCACA GGATCTTTGA CCAAAACCTG CTGGTCCCTC CGCACCCACA GAGAGCACGC 
CAGCCTCTGA AGGAATCGAC GGCGTTTCAG TGTGTCCTGA AGTGGCTTGA TGGCCCGCTA 
ATTAAGCAGG GGGTGCTGGA GCTGCTATCG GAGGTGGGCT GCCACCTGCG CGTGTCTCTC 
TTCGATGTCA CCTATCGGCA CTTCTTCGGG AGGACGTGGA GGAGCTCGCC GAGGCCTGCA 
GAGCCGCTCA CCAGGCAGCC GGCCAGGATC GTCTTCAATG AGCCCTTGTA TTTTCACACG 
TCCTTGAACC ACCCTCATAT TGTGGCCGTG GTGGAGGTGG TCGTTGAAGG CAGAAGACGA 
GACGGGACCC TCCAGACTCT GTCCTGTGGG TTTGGAATTC TTCGGATCTT CAGCCACAAG 
CCGGAATCTC CAACTTCCAC TTCCCAGGAC AAACGGCTGA GACTGTATCA TGGGAGCCCG 
CGAGCCCTCC TCCACCCGCT CCTTCAGGAC CCCATAGAAC AGAACAAGCA CATGACCCTC 
ATTGAGAACT GCAGCCTGCA GTACACGCTG AGGCCACACC CGCTCCTGGA GCCCGTGTTC 
CACCTTCTTC CCGAGAACTT GCTGGTGTCC GGTCTGCAGC AGATACCTGG CCTCCTTCCA 
GCTCACGGGG AATCAGGAGA TGCCCTCCGG AAGCCCCGCC TGCAGAAGCC TCTGACGTGC 
TACCTGGATG ATCTGTTCTT CACGCTGTAT CCCTCGCTGG AGAAGTTCGA GGAGGAGCTT 
CTGGAACTCG TGATCAGCGA TTGCTTCCGG GAGGCGGGCA GCCCGCTGGA CGTCGGCACC 
CTGGAGATCC TGGAGCGGCG CCTGTGCGTG GGCGTGCACA ATGGGTTGGG CTTCGTGCAG 
CGGCCGCAGG TCCTCGTGCT GGTGCCCGAG CTGGAGGTGG CCTTGACACG CTCTGCCAGC 
TTCAGCCGGA AGGTCGGCTC CTCTTCCAAG GCCAGCTCTG GAAACCAGGC GCTGGTCTTG 
AGAAGCCGTC TCCGACTCCC TGAGATGGTC CGTCACCCCG CGTTCGCCAT TGTCTTCCAG 
CTGGAGTACG TGTTTAACAG CCCCTCGGGG GCAGACAGCA ATGCAGCTTC ACTCTCCTCC 
TTGTCCAGCC TGGCATGTAT GCACATGGTT CGCTGGGCTG TCTGGAACCC CTCGCTGGAA 
GCCAGCTCCG GGCAGGTGAT GTTACCTCTC CAGGGTGGCA TCCAGTCCAA CCCTTCGCAC 
TGTCTGGTCT ATAAGGTACC CCCAGCCAGC ATAAGCTCCG AAGAGGTGAA GCAGGTGGAG 
TCAGGGACGG TCCAGTTCCA GTACTCGCTG AGCCCTGAAG AACACCCAGG CACACCCACA 
GCGAATGCAA GCAGCCCCAA GGCAGAGCGG CGGTCTTTTA GGAAGCCACC CGTGTCCCCT 
TCAAGTCCAC CAGCATCGCC GTCCCAGGGG CTCACCGCTG CCCAGGACTC ACCCATGGGA 
CCAGGGCTGT CACTTTCTCA GCTGGCGGCT TCCCCACCAT CCCCAGCTCC CCGCCGCCGC 
TCGGCCAAAC CTTCGTCACA GCCACCTGGG AGCCCTGTGC CCTCCCTGGT CCCAGGGGAG 
TTCCCCTTGG AGGGCAGGAT CTCTCACCTG GAGGCTGACC TGAGCCAGAC AGCCTTAGCC 
CTGGGAACGT CCGCTGCTGA CCACTTGCAG GAACTGCCTT TCACACCCGT GCACGCCCCG 
ATCATTGCAG GAACCCAGAC CAGGAGCTCT GGAGGGAAGC TGTCACGGGC ATCCCTGGCG 
CTGCTGCAGT CCGCTGGCTT TCCCGAGATT CTGGACGCCA ACAAGCAACC AGCTGAGGCT 
GTGAGTCCTA CAGACCCCGT GAAGTTCAGC CCTCAGAAGG AAGAATCAGA CTGTCTACAA 
GGCAATGAGA TAGTGTTGCA ATTTCTTGCC TTTAGCAGGG TGCCCCAGGA TGAGCAGGCA 
GCGCCATGGC CACAGACTGT GTACTTCACC TTCCAATTCT ACCGCTTCCC ACCCGAGACC 
ACGCCACGGC TGCAGCTCGT GGAGCTGGCC GATGCTGGGA AGACCAGCTC TGCCTCCCTG 
TCGCACATCC TCGCCCAGAT CCAGAAGGAT GGCTCCTTTG ATGCGGGGTC TCCGGGCTTT 
CAGCGGAAGT ACCTGGTGGA CCCCGGGTTC CTGAGACCCG GGGAGCAGCG CTGGTTCGCC 
CGCTACCTGG CCATGCAGAC CCTGCAGATC GACGTCTGGG ATGGGGACTC CCTGCTTCTC 
ATTGGATCTG CTGCCGTTCA GCTGAAGCAC CTTCTCCGCC AGGGGCGGCC GGCCGTGCAG 
GTCTCGCATG AGCTGGAGGT TGTGGCGACT GAATACGAGC AGGACGCCAT GGTGGTGAGT 
GGAGACATGA CCAAGTTTGG CTGCGTCAGA CCCATCGACG TCCACACGGT GGTGAAGGGC 
CAGCTGCACT TGACCTTGGC CAACGTGGGT CACTTGTGTG AACAGAGAGT AAGGAGTCCC 
AGTTCCCTGC CACCTTCCAG GTCGAGGGTC ATCTCAAATG ATGGAGTTGG CAACTTCATG 
GGAGGCAGCC TCCTGACACC CGGGGGCCCC AGATCAGTGA AAAATGTGGT CCAAGCTCAG 
AAGCTGGCTG ATGTGGACAG CGAACTGGCC GCCCTGCTCT TCACACAACC GAGACCAGGG 
GCCCGGGGGC CCCAGGGCAT CAATCAAGAG GGGGATGCCG TCCGCAGGCG CAAGCTGGAG 
CGCATGAGGG CCGTGCGGCT GCGGGAATGT GGATTGGAGC CGGGCCGCCG CGGGCTGGGC 
GCGCTGGCCC AGCAGAGCCT CCATGCCCAG CACGCCCGAG ACCTGCAGGT CATTGCCGCC 
TACCGTGAGC GCACAAAGGC GGAGAGCATC GCCAGCGCAC TGAGCACGGC CATCACCACG 
CAGCACACCA TCCACGCCAC CCTAGGCGCT GCCGAGTTCT TCGAGTTCGC CCTGAGGAAC 
CCCCACAACG CGCAGCACAT GGTGACCATC GAGATAGACA ACCCCGAGCT CAGTCTCATC 
CTGGATGGTC AGGAGTGGCG GTACTTCAAG GACATGGCTG ACTTGCACAC ACCCCTGGAG 
GAGGACATGT TCCACCTGCG GGGCAGCCTG GCCCCCCAAC TCTTCCTGCG ACCTCGGGAG 
ACTGCCCACA TCCCCTTCAA GTTCCAGACT TTCTCTGTGG GCCCCTGGGC CTCGGCACAG 
GCCTCTGCTG AGCTGAGATC AGAGAAGGAC ATGGATGCCG GATCACCCTA TAAGTCCAGC 
ATGATGCCCA CCAAACACGC CAAGGTCCTG TTCCGCGCGG GCAGCGGCAA GCCCCTGGCC 
GTGCTCTGCC TGACGGTGGA GCTGCAGCCT CACGTGGTGG ACCAGGTCTT CCGCTTCCAC 
CACCCGGAGC TCACCTTCCT GAAGAAAGCC ATCCGCCTGC CACCCTGGCA CACGCTTCCA 
GGTGCCCCTG TAGGGACTTC CAGTGAGGAC CCCCCTTTCC ATGTTCGATG TAGTGACCCG 
AACATCATTT GTGAGACACA GAGTGTGGGC CCCGGGGAGC CCCGTGACGT GTTCCTGAAG 
GTGGCCAGTG GCCCTAGCCC AGAGGTCAAG GACTTCTTTG TCACCATCTA CGTAGACCGC 
TGGTTAGCAA CACCTGTCCA GACCTGGCAG GTCCACCTGC ACTCGCTGCA GCGAGTGGAC 
GTCTCCTGTA TCACTGGCCA GAGGACCCGC CTGGCTCTCG TCCTCCGGGG GACGCAGACA 
GTTAGGAGAG TGCAGGCTTT CAGCTCACAC CCCCAGGAGC TGACGACGGA CCCCAGAGGT 
GTGTTCGTGC TGCCTCCTCA CGGGGTGCAG GACTTGCATG TGAGCGTCAG GCCCTGCAGG 
CCGGGCAGCC GCTTCGCGCA CCTCCACCTG GTTGACGTGG ACGCCCACCA GCTGGTGGCC 
TCGTGGCTCC TGTGTCTCTC CTGCCGGCCG CCTCTCATCT CCAAGGCCTT CGAGATAAAG 
CTGCCCGCGG GGAGCGGGAG GGGCGCCAAT AAGCGCATCA CCTACACCAA TCCCTACCCC 
TCGCGGAGGG CCTACCGCCT GCACAGCGAC CACCCCCACC TGCTGCAGTT CAAGGAGGAC 
GCCTTCCAGG TCGGGGGAGG TGAGACCTAT ACCATCGGCC TGCGGTTTGT GTCCCGTCAG 
AGAGCCGGGG AGGAAGAGAT CCTGATTTAC ATCAACGACC ACGAAGACAA AAACGAGGAG 
ACCTTTTGCG TGAAGGTCAT CTACCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015330664.1
CDS	581..5110
Translation	MIVPSPPVPSRVSITAPCTLLSSVIFIGHNHLFCVLTCILCFCLPSDTFLEVIVVVCSVLQGLAQGNPRGPRDPGTSRMHDWHRIFDQNLLVPPHPQRARQPLKESTAFQCVLKWLDGPLIKQGVLELLSEVGCHLRVSLFDVTYRHFFGRTWRSSPRPAEPLTRQPARIVFNEPLYFHTSLNHPHIVAVVEVVVEGRRRDGTLQTLSCGFGILRIFSHKPESPTSTSQDKRLRLYHGSPRALLHPLLQDPIEQNKHMTLIENCSLQYTLRPHPLLEPVFHLLPENLLVSGLQQIPGLLPAHGESGDALRKPRLQKPLTCYLDDLFFTLYPSLEKFEEELLELVISDCFREAGSPLDVGTLEILERRLCVGVHNGLGFVQRPQVLVLVPELEVALTRSASFSRKVGSSSKASSGNQALVLRSRLRLPEMVRHPAFAIVFQLEYVFNSPSGADSNAASLSSLSSLACMHMVRWAVWNPSLEASSGQVMLPLQGGIQSNPSHCLVYKVPPASISSEEVKQVESGTVQFQYSLSPEEHPGTPTANASSPKAERRSFRKPPVSPSSPPASPSQGLTAAQDSPMGPGLSLSQLAASPPSPAPRRRSAKPSSQPPGSPVPSLVPGEFPLEGRISHLEADLSQTALALGTSAADHLQELPFTPVHAPIIAGTQTRSSGGKLSRASLALLQSAGFPEILDANKQPAEAVSPTDPVKFSPQKEESDCLQGNEIVLQFLAFSRVPQDEQAAPWPQTVYFTFQFYRFPPETTPRLQLVELADAGKTSSASLSHILAQIQKDGSFDAGSPGFQRKYLVDPGFLRPGEQRWFARYLAMQTLQIDVWDGDSLLLIGSAAVQLKHLLRQGRPAVQVSHELEVVATEYEQDAMVVSGDMTKFGCVRPIDVHTVVKGQLHLTLANVGHLCEQRVRSPSSLPPSRSRVISNDGVGNFMGGSLLTPGGPRSVKNVVQAQKLADVDSELAALLFTQPRPGARGPQGINQEGDAVRRRKLERMRAVRLRECGLEPGRRGLGALAQQSLHAQHARDLQVIAAYRERTKAESIASALSTAITTQHTIHATLGAAEFFEFALRNPHNAQHMVTIEIDNPELSLILDGQEWRYFKDMADLHTPLEEDMFHLRGSLAPQLFLRPRETAHIPFKFQTFSVGPWASAQASAELRSEKDMDAGSPYKSSMMPTKHAKVLFRAGSGKPLAVLCLTVELQPHVVDQVFRFHHPELTFLKKAIRLPPWHTLPGAPVGTSSEDPPFHVRCSDPNIICETQSVGPGEPRDVFLKVASGPSPEVKDFFVTIYVDRWLATPVQTWQVHLHSLQRVDVSCITGQRTRLALVLRGTQTVRRVQAFSSHPQELTTDPRGVFVLPPHGVQDLHVSVRPCRPGSRFAHLHLVDVDAHQLVASWLLCLSCRPPLISKAFEIKLPAGSGRGANKRITYTNPYPSRRAYRLHSDHPHLLQFKEDAFQVGGGETYTIGLRFVSRQRAGEEEILIYINDHEDKNEETFCVKVIYQ

Target ORF information:

RefSeq Version	XM_015475178.1
Organism	Bos taurus(cattle)
Definition	Bos taurus nephronophthisis 4 (NPHP4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015475178.1

ORF Insert Sequence:

ATGATTGTGC CGTCGCCGCC AGTTCCAAGC CGGGTCTCCA TCACAGCTCC CTGCACCCTC 
CTCTCCTCTG TGATTTTCAT AGGTCACAAC CACCTCTTTT GTGTGCTAAC TTGTATCCTG 
TGTTTCTGCC TCCCTTCAGA TACATTCCTT GAGGTCATCG TGGTTGTCTG CAGTGTTCTC 
CAGGGCCTGG CACAGGGTAA TCCCAGAGGC CCCAGGGACC CGGGGACCAG CAGAATGCAC 
GACTGGCACA GGATCTTTGA CCAAAACCTG CTGGTCCCTC CGCACCCACA GAGAGCACGC 
CAGCCTCTGA AGGAATCGAC GGCGTTTCAG TGTGTCCTGA AGTGGCTTGA TGGCCCGCTA 
ATTAAGCAGG GGGTGCTGGA GCTGCTATCG GAGGTGGGCT GCCACCTGCG CGTGTCTCTC 
TTCGATGTCA CCTATCGGCA CTTCTTCGGG AGGACGTGGA GGAGCTCGCC GAGGCCTGCA 
GAGCCGCTCA CCAGGCAGCC GGCCAGGATC GTCTTCAATG AGCCCTTGTA TTTTCACACG 
TCCTTGAACC ACCCTCATAT TGTGGCCGTG GTGGAGGTGG TCGTTGAAGG CAGAAGACGA 
GACGGGACCC TCCAGACTCT GTCCTGTGGG TTTGGAATTC TTCGGATCTT CAGCCACAAG 
CCGGAATCTC CAACTTCCAC TTCCCAGGAC AAACGGCTGA GACTGTATCA TGGGAGCCCG 
CGAGCCCTCC TCCACCCGCT CCTTCAGGAC CCCATAGAAC AGAACAAGCA CATGACCCTC 
ATTGAGAACT GCAGCCTGCA GTACACGCTG AGGCCACACC CGCTCCTGGA GCCCGTGTTC 
CACCTTCTTC CCGAGAACTT GCTGGTGTCC GGTCTGCAGC AGATACCTGG CCTCCTTCCA 
GCTCACGGGG AATCAGGAGA TGCCCTCCGG AAGCCCCGCC TGCAGAAGCC TCTGACGTGC 
TACCTGGATG ATCTGTTCTT CACGCTGTAT CCCTCGCTGG AGAAGTTCGA GGAGGAGCTT 
CTGGAACTCG TGATCAGCGA TTGCTTCCGG GAGGCGGGCA GCCCGCTGGA CGTCGGCACC 
CTGGAGATCC TGGAGCGGCG CCTGTGCGTG GGCGTGCACA ATGGGTTGGG CTTCGTGCAG 
CGGCCGCAGG TCCTCGTGCT GGTGCCCGAG CTGGAGGTGG CCTTGACACG CTCTGCCAGC 
TTCAGCCGGA AGGTCGGCTC CTCTTCCAAG GCCAGCTCTG GAAACCAGGC GCTGGTCTTG 
AGAAGCCGTC TCCGACTCCC TGAGATGGTC CGTCACCCCG CGTTCGCCAT TGTCTTCCAG 
CTGGAGTACG TGTTTAACAG CCCCTCGGGG GCAGACAGCA ATGCAGCTTC ACTCTCCTCC 
TTGTCCAGCC TGGCATGTAT GCACATGGTT CGCTGGGCTG TCTGGAACCC CTCGCTGGAA 
GCCAGCTCCG GGCAGGTGAT GTTACCTCTC CAGGGTGGCA TCCAGTCCAA CCCTTCGCAC 
TGTCTGGTCT ATAAGGTACC CCCAGCCAGC ATAAGCTCCG AAGAGGTGAA GCAGGTGGAG 
TCAGGGACGG TCCAGTTCCA GTACTCGCTG AGCCCTGAAG AACACCCAGG CACACCCACA 
GCGAATGCAA GCAGCCCCAA GGCAGAGCGG CGGTCTTTTA GGAAGCCACC CGTGTCCCCT 
TCAAGTCCAC CAGCATCGCC GTCCCAGGGG CTCACCGCTG CCCAGGACTC ACCCATGGGA 
CCAGGGCTGT CACTTTCTCA GCTGGCGGCT TCCCCACCAT CCCCAGCTCC CCGCCGCCGC 
TCGGCCAAAC CTTCGTCACA GCCACCTGGG AGCCCTGTGC CCTCCCTGGT CCCAGGGGAG 
TTCCCCTTGG AGGGCAGGAT CTCTCACCTG GAGGCTGACC TGAGCCAGAC AGCCTTAGCC 
CTGGGAACGT CCGCTGCTGA CCACTTGCAG GAACTGCCTT TCACACCCGT GCACGCCCCG 
ATCATTGCAG GAACCCAGAC CAGGAGCTCT GGAGGGAAGC TGTCACGGGC ATCCCTGGCG 
CTGCTGCAGT CCGCTGGCTT TCCCGAGATT CTGGACGCCA ACAAGCAACC AGCTGAGGCT 
GTGAGTCCTA CAGACCCCGT GAAGTTCAGC CCTCAGAAGG AAGAATCAGA CTGTCTACAA 
GGCAATGAGA TAGTGTTGCA ATTTCTTGCC TTTAGCAGGG TGCCCCAGGA TGAGCAGGCA 
GCGCCATGGC CACAGACTGT GTACTTCACC TTCCAATTCT ACCGCTTCCC ACCCGAGACC 
ACGCCACGGC TGCAGCTCGT GGAGCTGGCC GATGCTGGGA AGACCAGCTC TGCCTCCCTG 
TCGCACATCC TCGCCCAGAT CCAGAAGGAT GGCTCCTTTG ATGCGGGGTC TCCGGGCTTT 
CAGCGGAAGT ACCTGGTGGA CCCCGGGTTC CTGAGACCCG GGGAGCAGCG CTGGTTCGCC 
CGCTACCTGG CCATGCAGAC CCTGCAGATC GACGTCTGGG ATGGGGACTC CCTGCTTCTC 
ATTGGATCTG CTGCCGTTCA GCTGAAGCAC CTTCTCCGCC AGGGGCGGCC GGCCGTGCAG 
GTCTCGCATG AGCTGGAGGT TGTGGCGACT GAATACGAGC AGGACGCCAT GGTGGTGAGT 
GGAGACATGA CCAAGTTTGG CTGCGTCAGA CCCATCGACG TCCACACGGT GGTGAAGGGC 
CAGCTGCACT TGACCTTGGC CAACGTGGGT CACTTGTGTG AACAGAGAGT AAGGAGTCCC 
AGTTCCCTGC CACCTTCCAG GTCGAGGGTC ATCTCAAATG ATGGAGTTGG CAACTTCATG 
GGAGGCAGCC TCCTGACACC CGGGGGCCCC AGATCAGTGA AAAATGTGGT CCAAGCTCAG 
AAGCTGGCTG ATGTGGACAG CGAACTGGCC GCCCTGCTCT TCACACAACC GAGACCAGGG 
GCCCGGGGGC CCCAGGGCAT CAATCAAGAG GGGGATGCCG TCCGCAGGCG CAAGCTGGAG 
CGCATGAGGG CCGTGCGGCT GCGGGAATGT GGATTGGAGC CGGGCCGCCG CGGGCTGGGC 
GCGCTGGCCC AGCAGAGCCT CCATGCCCAG CACGCCCGAG ACCTGCAGGT CATTGCCGCC 
TACCGTGAGC GCACAAAGGC GGAGAGCATC GCCAGCGCAC TGAGCACGGC CATCACCACG 
CAGCACACCA TCCACGCCAC CCTAGGCGCT GCCGAGTTCT TCGAGTTCGC CCTGAGGAAC 
CCCCACAACG CGCAGCACAT GGTGACCATC GAGATAGACA ACCCCGAGCT CAGTCTCATC 
CTGGATGGTC AGGAGTGGCG GTACTTCAAG GACATGGCTG ACTTGCACAC ACCCCTGGAG 
GAGGACATGT TCCACCTGCG GGGCAGCCTG GCCCCCCAAC TCTTCCTGCG ACCTCGGGAG 
ACTGCCCACA TCCCCTTCAA GTTCCAGACT TTCTCTGTGG GCCCCTGGGC CTCGGCACAG 
GCCTCTGCTG AGCTGAGATC AGAGAAGGAC ATGGATGCCG GATCACCCTA TAAGTCCAGC 
ATGATGCCCA CCAAACACGC CAAGGTCCTG TTCCGCGCGG GCAGCGGCAA GCCCCTGGCC 
GTGCTCTGCC TGACGGTGGA GCTGCAGCCT CACGTGGTGG ACCAGGTCTT CCGCTTCCAC 
CACCCGGAGC TCACCTTCCT GAAGAAAGCC ATCCGCCTGC CACCCTGGCA CACGCTTCCA 
GGTGCCCCTG TAGGGACTTC CAGTGAGGAC CCCCCTTTCC ATGTTCGATG TAGTGACCCG 
AACATCATTT GTGAGACACA GAGTGTGGGC CCCGGGGAGC CCCGTGACGT GTTCCTGAAG 
GTGGCCAGTG GCCCTAGCCC AGAGGTCAAG GACTTCTTTG TCACCATCTA CGTAGACCGC 
TGGTTAGCAA CACCTGTCCA GACCTGGCAG GTCCACCTGC ACTCGCTGCA GCGAGTGGAC 
GTCTCCTGTA TCACTGGCCA GAGGACCCGC CTGGCTCTCG TCCTCCGGGG GACGCAGACA 
GTTAGGAGAG TGCAGGCTTT CAGCTCACAC CCCCAGGAGC TGACGACGGA CCCCAGAGGT 
GTGTTCGTGC TGCCTCCTCA CGGGGTGCAG GACTTGCATG TGAGCGTCAG GCCCTGCAGG 
CCGGGCAGCC GCTTCGCGCA CCTCCACCTG GTTGACGTGG ACGCCCACCA GCTGGTGGCC 
TCGTGGCTCC TGTGTCTCTC CTGCCGGCCG CCTCTCATCT CCAAGGCCTT CGAGATAAAG 
CTGCCCGCGG GGAGCGGGAG GGGCGCCAAT AAGCGCATCA CCTACACCAA TCCCTACCCC 
TCGCGGAGGG CCTACCGCCT GCACAGCGAC CACCCCCACC TGCTGCAGTT CAAGGAGGAC 
GCCTTCCAGG TCGGGGGAGG TGAGACCTAT ACCATCGGCC TGCGGTTTGT GTCCCGTCAG 
AGAGCCGGGG AGGAAGAGAT CCTGATTTAC ATCAACGACC ACGAAGACAA AAACGAGGAG 
ACCTTTTGCG TGAAGGTCAT CTACCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPHP4 ( XM_015475180.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015330666.1 Bos taurus nephronophthisis 4 (NPHP4), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa27367
Clone ID Related Accession (Same CDS sequence)	XM_015475180.1 , XM_015466842.1 , XM_015466845.1 , XM_024976773.1 , XM_005217172.4 , XM_015475179.1 , XM_015466844.1 , XM_015466846.1 , XM_005217172.3 , XM_010813390.2 , XM_024976775.1 , XM_024976776.1 , XM_005217171.3 , XM_024976772.1 , XM_010813390.3 , XM_015466843.1 , XM_005217171.4 , XM_015475180.2 , XM_024976774.1
Accession Version	XM_015475180.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4296bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	nephrocystin-4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104439.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACGACT GGCACAGGAT CTTTGACCAA AACCTGCTGG TCCCTCCGCA CCCACAGAGA 
GCACGCCAGC CTCTGAAGGA ATCGACGGCG TTTCAGTGTG TCCTGAAGTG GCTTGATGGC 
CCGCTAATTA AGCAGGGGGT GCTGGAGCTG CTATCGGAGG TGGGCTGCCA CCTGCGCGTG 
TCTCTCTTCG ATGTCACCTA TCGGCACTTC TTCGGGAGGA CGTGGAGGAG CTCGCCGAGG 
CCTGCAGAGC CGCTCACCAG GCAGCCGGCC AGGATCGTCT TCAATGAGCC CTTGTATTTT 
CACACGTCCT TGAACCACCC TCATATTGTG GCCGTGGTGG AGGTGGTCGT TGAAGGCAGA 
AGACGAGACG GGACCCTCCA GACTCTGTCC TGTGGGTTTG GAATTCTTCG GATCTTCAGC 
CACAAGCCGG AATCTCCAAC TTCCACTTCC CAGGACAAAC GGCTGAGACT GTATCATGGG 
AGCCCGCGAG CCCTCCTCCA CCCGCTCCTT CAGGACCCCA TAGAACAGAA CAAGCACATG 
ACCCTCATTG AGAACTGCAG CCTGCAGTAC ACGCTGAGGC CACACCCGCT CCTGGAGCCC 
GTGTTCCACC TTCTTCCCGA GAACTTGCTG GTGTCCGGTC TGCAGCAGAT ACCTGGCCTC 
CTTCCAGCTC ACGGGGAATC AGGAGATGCC CTCCGGAAGC CCCGCCTGCA GAAGCCTCTG 
ACGTGCTACC TGGATGATCT GTTCTTCACG CTGTATCCCT CGCTGGAGAA GTTCGAGGAG 
GAGCTTCTGG AACTCGTGAT CAGCGATTGC TTCCGGGAGG CGGGCAGCCC GCTGGACGTC 
GGCACCCTGG AGATCCTGGA GCGGCGCCTG TGCGTGGGCG TGCACAATGG GTTGGGCTTC 
GTGCAGCGGC CGCAGGTCCT CGTGCTGGTG CCCGAGCTGG AGGTGGCCTT GACACGCTCT 
GCCAGCTTCA GCCGGAAGGT CGGCTCCTCT TCCAAGGCCA GCTCTGGAAA CCAGGCGCTG 
GTCTTGAGAA GCCGTCTCCG ACTCCCTGAG ATGGTCCGTC ACCCCGCGTT CGCCATTGTC 
TTCCAGCTGG AGTACGTGTT TAACAGCCCC TCGGGGGCAG ACAGCAATGC AGCTTCACTC 
TCCTCCTTGT CCAGCCTGGC ATGTATGCAC ATGGTTCGCT GGGCTGTCTG GAACCCCTCG 
CTGGAAGCCA GCTCCGGGCA GGTGATGTTA CCTCTCCAGG GTGGCATCCA GTCCAACCCT 
TCGCACTGTC TGGTCTATAA GGTACCCCCA GCCAGCATAA GCTCCGAAGA GGTGAAGCAG 
GTGGAGTCAG GGACGGTCCA GTTCCAGTAC TCGCTGAGCC CTGAAGAACA CCCAGGCACA 
CCCACAGCGA ATGCAAGCAG CCCCAAGGCA GAGCGGCGGT CTTTTAGGAA GCCACCCGTG 
TCCCCTTCAA GTCCACCAGC ATCGCCGTCC CAGGGGCTCA CCGCTGCCCA GGACTCACCC 
ATGGGACCAG GGCTGTCACT TTCTCAGCTG GCGGCTTCCC CACCATCCCC AGCTCCCCGC 
CGCCGCTCGG CCAAACCTTC GTCACAGCCA CCTGGGAGCC CTGTGCCCTC CCTGGTCCCA 
GGGGAGTTCC CCTTGGAGGG CAGGATCTCT CACCTGGAGG CTGACCTGAG CCAGACAGCC 
TTAGCCCTGG GAACGTCCGC TGCTGACCAC TTGCAGGAAC TGCCTTTCAC ACCCGTGCAC 
GCCCCGATCA TTGCAGGAAC CCAGACCAGG AGCTCTGGAG GGAAGCTGTC ACGGGCATCC 
CTGGCGCTGC TGCAGTCCGC TGGCTTTCCC GAGATTCTGG ACGCCAACAA GCAACCAGCT 
GAGGCTGTGA GTCCTACAGA CCCCGTGAAG TTCAGCCCTC AGAAGGAAGA ATCAGACTGT 
CTACAAGGCA ATGAGATAGT GTTGCAATTT CTTGCCTTTA GCAGGGTGCC CCAGGATGAG 
CAGGCAGCGC CATGGCCACA GACTGTGTAC TTCACCTTCC AATTCTACCG CTTCCCACCC 
GAGACCACGC CACGGCTGCA GCTCGTGGAG CTGGCCGATG CTGGGAAGAC CAGCTCTGCC 
TCCCTGTCGC ACATCCTCGC CCAGATCCAG AAGGATGGCT CCTTTGATGC GGGGTCTCCG 
GGCTTTCAGC GGAAGTACCT GGTGGACCCC GGGTTCCTGA GACCCGGGGA GCAGCGCTGG 
TTCGCCCGCT ACCTGGCCAT GCAGACCCTG CAGATCGACG TCTGGGATGG GGACTCCCTG 
CTTCTCATTG GATCTGCTGC CGTTCAGCTG AAGCACCTTC TCCGCCAGGG GCGGCCGGCC 
GTGCAGGTCT CGCATGAGCT GGAGGTTGTG GCGACTGAAT ACGAGCAGGA CGCCATGGTG 
GTGAGTGGAG ACATGACCAA GTTTGGCTGC GTCAGACCCA TCGACGTCCA CACGGTGGTG 
AAGGGCCAGC TGCACTTGAC CTTGGCCAAC GTGGGTCACT TGTGTGAACA GAGAGTAAGG 
AGTCCCAGTT CCCTGCCACC TTCCAGGTCG AGGGTCATCT CAAATGATGG AGTTGGCAAC 
TTCATGGGAG GCAGCCTCCT GACACCCGGG GGCCCCAGAT CAGTGAAAAA TGTGGTCCAA 
GCTCAGAAGC TGGCTGATGT GGACAGCGAA CTGGCCGCCC TGCTCTTCAC ACAACCGAGA 
CCAGGGGCCC GGGGGCCCCA GGGCATCAAT CAAGAGGGGG ATGCCGTCCG CAGGCGCAAG 
CTGGAGCGCA TGAGGGCCGT GCGGCTGCGG GAATGTGGAT TGGAGCCGGG CCGCCGCGGG 
CTGGGCGCGC TGGCCCAGCA GAGCCTCCAT GCCCAGCACG CCCGAGACCT GCAGGTCATT 
GCCGCCTACC GTGAGCGCAC AAAGGCGGAG AGCATCGCCA GCGCACTGAG CACGGCCATC 
ACCACGCAGC ACACCATCCA CGCCACCCTA GGCGCTGCCG AGTTCTTCGA GTTCGCCCTG 
AGGAACCCCC ACAACGCGCA GCACATGGTG ACCATCGAGA TAGACAACCC CGAGCTCAGT 
CTCATCCTGG ATGGTCAGGA GTGGCGGTAC TTCAAGGACA TGGCTGACTT GCACACACCC 
CTGGAGGAGG ACATGTTCCA CCTGCGGGGC AGCCTGGCCC CCCAACTCTT CCTGCGACCT 
CGGGAGACTG CCCACATCCC CTTCAAGTTC CAGACTTTCT CTGTGGGCCC CTGGGCCTCG 
GCACAGGCCT CTGCTGAGCT GAGATCAGAG AAGGACATGG ATGCCGGATC ACCCTATAAG 
TCCAGCATGA TGCCCACCAA ACACGCCAAG GTCCTGTTCC GCGCGGGCAG CGGCAAGCCC 
CTGGCCGTGC TCTGCCTGAC GGTGGAGCTG CAGCCTCACG TGGTGGACCA GGTCTTCCGC 
TTCCACCACC CGGAGCTCAC CTTCCTGAAG AAAGCCATCC GCCTGCCACC CTGGCACACG 
CTTCCAGGTG CCCCTGTAGG GACTTCCAGT GAGGACCCCC CTTTCCATGT TCGATGTAGT 
GACCCGAACA TCATTTGTGA GACACAGAGT GTGGGCCCCG GGGAGCCCCG TGACGTGTTC 
CTGAAGGTGG CCAGTGGCCC TAGCCCAGAG GTCAAGGACT TCTTTGTCAC CATCTACGTA 
GACCGCTGGT TAGCAACACC TGTCCAGACC TGGCAGGTCC ACCTGCACTC GCTGCAGCGA 
GTGGACGTCT CCTGTATCAC TGGCCAGAGG ACCCGCCTGG CTCTCGTCCT CCGGGGGACG 
CAGACAGTTA GGAGAGTGCA GGCTTTCAGC TCACACCCCC AGGAGCTGAC GACGGACCCC 
AGAGGTGTGT TCGTGCTGCC TCCTCACGGG GTGCAGGACT TGCATGTGAG CGTCAGGCCC 
TGCAGGCCGG GCAGCCGCTT CGCGCACCTC CACCTGGTTG ACGTGGACGC CCACCAGCTG 
GTGGCCTCGT GGCTCCTGTG TCTCTCCTGC CGGCCGCCTC TCATCTCCAA GGCCTTCGAG 
ATAAAGCTGC CCGCGGGGAG CGGGAGGGGC GCCAATAAGC GCATCACCTA CACCAATCCC 
TACCCCTCGC GGAGGGCCTA CCGCCTGCAC AGCGACCACC CCCACCTGCT GCAGTTCAAG 
GAGGACGCCT TCCAGGTCGG GGGAGGTGAG ACCTATACCA TCGGCCTGCG GTTTGTGTCC 
CGTCAGAGAG CCGGGGAGGA AGAGATCCTG ATTTACATCA ACGACCACGA AGACAAAAAC 
GAGGAGACCT TTTGCGTGAA GGTCATCTAC CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015330666.1
CDS	1032..5327
Translation	MHDWHRIFDQNLLVPPHPQRARQPLKESTAFQCVLKWLDGPLIKQGVLELLSEVGCHLRVSLFDVTYRHFFGRTWRSSPRPAEPLTRQPARIVFNEPLYFHTSLNHPHIVAVVEVVVEGRRRDGTLQTLSCGFGILRIFSHKPESPTSTSQDKRLRLYHGSPRALLHPLLQDPIEQNKHMTLIENCSLQYTLRPHPLLEPVFHLLPENLLVSGLQQIPGLLPAHGESGDALRKPRLQKPLTCYLDDLFFTLYPSLEKFEEELLELVISDCFREAGSPLDVGTLEILERRLCVGVHNGLGFVQRPQVLVLVPELEVALTRSASFSRKVGSSSKASSGNQALVLRSRLRLPEMVRHPAFAIVFQLEYVFNSPSGADSNAASLSSLSSLACMHMVRWAVWNPSLEASSGQVMLPLQGGIQSNPSHCLVYKVPPASISSEEVKQVESGTVQFQYSLSPEEHPGTPTANASSPKAERRSFRKPPVSPSSPPASPSQGLTAAQDSPMGPGLSLSQLAASPPSPAPRRRSAKPSSQPPGSPVPSLVPGEFPLEGRISHLEADLSQTALALGTSAADHLQELPFTPVHAPIIAGTQTRSSGGKLSRASLALLQSAGFPEILDANKQPAEAVSPTDPVKFSPQKEESDCLQGNEIVLQFLAFSRVPQDEQAAPWPQTVYFTFQFYRFPPETTPRLQLVELADAGKTSSASLSHILAQIQKDGSFDAGSPGFQRKYLVDPGFLRPGEQRWFARYLAMQTLQIDVWDGDSLLLIGSAAVQLKHLLRQGRPAVQVSHELEVVATEYEQDAMVVSGDMTKFGCVRPIDVHTVVKGQLHLTLANVGHLCEQRVRSPSSLPPSRSRVISNDGVGNFMGGSLLTPGGPRSVKNVVQAQKLADVDSELAALLFTQPRPGARGPQGINQEGDAVRRRKLERMRAVRLRECGLEPGRRGLGALAQQSLHAQHARDLQVIAAYRERTKAESIASALSTAITTQHTIHATLGAAEFFEFALRNPHNAQHMVTIEIDNPELSLILDGQEWRYFKDMADLHTPLEEDMFHLRGSLAPQLFLRPRETAHIPFKFQTFSVGPWASAQASAELRSEKDMDAGSPYKSSMMPTKHAKVLFRAGSGKPLAVLCLTVELQPHVVDQVFRFHHPELTFLKKAIRLPPWHTLPGAPVGTSSEDPPFHVRCSDPNIICETQSVGPGEPRDVFLKVASGPSPEVKDFFVTIYVDRWLATPVQTWQVHLHSLQRVDVSCITGQRTRLALVLRGTQTVRRVQAFSSHPQELTTDPRGVFVLPPHGVQDLHVSVRPCRPGSRFAHLHLVDVDAHQLVASWLLCLSCRPPLISKAFEIKLPAGSGRGANKRITYTNPYPSRRAYRLHSDHPHLLQFKEDAFQVGGGETYTIGLRFVSRQRAGEEEILIYINDHEDKNEETFCVKVIYQ

Target ORF information:

RefSeq Version	XM_015475180.1
Organism	Bos taurus(cattle)
Definition	Bos taurus nephronophthisis 4 (NPHP4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015475180.1

ORF Insert Sequence:

ATGCACGACT GGCACAGGAT CTTTGACCAA AACCTGCTGG TCCCTCCGCA CCCACAGAGA 
GCACGCCAGC CTCTGAAGGA ATCGACGGCG TTTCAGTGTG TCCTGAAGTG GCTTGATGGC 
CCGCTAATTA AGCAGGGGGT GCTGGAGCTG CTATCGGAGG TGGGCTGCCA CCTGCGCGTG 
TCTCTCTTCG ATGTCACCTA TCGGCACTTC TTCGGGAGGA CGTGGAGGAG CTCGCCGAGG 
CCTGCAGAGC CGCTCACCAG GCAGCCGGCC AGGATCGTCT TCAATGAGCC CTTGTATTTT 
CACACGTCCT TGAACCACCC TCATATTGTG GCCGTGGTGG AGGTGGTCGT TGAAGGCAGA 
AGACGAGACG GGACCCTCCA GACTCTGTCC TGTGGGTTTG GAATTCTTCG GATCTTCAGC 
CACAAGCCGG AATCTCCAAC TTCCACTTCC CAGGACAAAC GGCTGAGACT GTATCATGGG 
AGCCCGCGAG CCCTCCTCCA CCCGCTCCTT CAGGACCCCA TAGAACAGAA CAAGCACATG 
ACCCTCATTG AGAACTGCAG CCTGCAGTAC ACGCTGAGGC CACACCCGCT CCTGGAGCCC 
GTGTTCCACC TTCTTCCCGA GAACTTGCTG GTGTCCGGTC TGCAGCAGAT ACCTGGCCTC 
CTTCCAGCTC ACGGGGAATC AGGAGATGCC CTCCGGAAGC CCCGCCTGCA GAAGCCTCTG 
ACGTGCTACC TGGATGATCT GTTCTTCACG CTGTATCCCT CGCTGGAGAA GTTCGAGGAG 
GAGCTTCTGG AACTCGTGAT CAGCGATTGC TTCCGGGAGG CGGGCAGCCC GCTGGACGTC 
GGCACCCTGG AGATCCTGGA GCGGCGCCTG TGCGTGGGCG TGCACAATGG GTTGGGCTTC 
GTGCAGCGGC CGCAGGTCCT CGTGCTGGTG CCCGAGCTGG AGGTGGCCTT GACACGCTCT 
GCCAGCTTCA GCCGGAAGGT CGGCTCCTCT TCCAAGGCCA GCTCTGGAAA CCAGGCGCTG 
GTCTTGAGAA GCCGTCTCCG ACTCCCTGAG ATGGTCCGTC ACCCCGCGTT CGCCATTGTC 
TTCCAGCTGG AGTACGTGTT TAACAGCCCC TCGGGGGCAG ACAGCAATGC AGCTTCACTC 
TCCTCCTTGT CCAGCCTGGC ATGTATGCAC ATGGTTCGCT GGGCTGTCTG GAACCCCTCG 
CTGGAAGCCA GCTCCGGGCA GGTGATGTTA CCTCTCCAGG GTGGCATCCA GTCCAACCCT 
TCGCACTGTC TGGTCTATAA GGTACCCCCA GCCAGCATAA GCTCCGAAGA GGTGAAGCAG 
GTGGAGTCAG GGACGGTCCA GTTCCAGTAC TCGCTGAGCC CTGAAGAACA CCCAGGCACA 
CCCACAGCGA ATGCAAGCAG CCCCAAGGCA GAGCGGCGGT CTTTTAGGAA GCCACCCGTG 
TCCCCTTCAA GTCCACCAGC ATCGCCGTCC CAGGGGCTCA CCGCTGCCCA GGACTCACCC 
ATGGGACCAG GGCTGTCACT TTCTCAGCTG GCGGCTTCCC CACCATCCCC AGCTCCCCGC 
CGCCGCTCGG CCAAACCTTC GTCACAGCCA CCTGGGAGCC CTGTGCCCTC CCTGGTCCCA 
GGGGAGTTCC CCTTGGAGGG CAGGATCTCT CACCTGGAGG CTGACCTGAG CCAGACAGCC 
TTAGCCCTGG GAACGTCCGC TGCTGACCAC TTGCAGGAAC TGCCTTTCAC ACCCGTGCAC 
GCCCCGATCA TTGCAGGAAC CCAGACCAGG AGCTCTGGAG GGAAGCTGTC ACGGGCATCC 
CTGGCGCTGC TGCAGTCCGC TGGCTTTCCC GAGATTCTGG ACGCCAACAA GCAACCAGCT 
GAGGCTGTGA GTCCTACAGA CCCCGTGAAG TTCAGCCCTC AGAAGGAAGA ATCAGACTGT 
CTACAAGGCA ATGAGATAGT GTTGCAATTT CTTGCCTTTA GCAGGGTGCC CCAGGATGAG 
CAGGCAGCGC CATGGCCACA GACTGTGTAC TTCACCTTCC AATTCTACCG CTTCCCACCC 
GAGACCACGC CACGGCTGCA GCTCGTGGAG CTGGCCGATG CTGGGAAGAC CAGCTCTGCC 
TCCCTGTCGC ACATCCTCGC CCAGATCCAG AAGGATGGCT CCTTTGATGC GGGGTCTCCG 
GGCTTTCAGC GGAAGTACCT GGTGGACCCC GGGTTCCTGA GACCCGGGGA GCAGCGCTGG 
TTCGCCCGCT ACCTGGCCAT GCAGACCCTG CAGATCGACG TCTGGGATGG GGACTCCCTG 
CTTCTCATTG GATCTGCTGC CGTTCAGCTG AAGCACCTTC TCCGCCAGGG GCGGCCGGCC 
GTGCAGGTCT CGCATGAGCT GGAGGTTGTG GCGACTGAAT ACGAGCAGGA CGCCATGGTG 
GTGAGTGGAG ACATGACCAA GTTTGGCTGC GTCAGACCCA TCGACGTCCA CACGGTGGTG 
AAGGGCCAGC TGCACTTGAC CTTGGCCAAC GTGGGTCACT TGTGTGAACA GAGAGTAAGG 
AGTCCCAGTT CCCTGCCACC TTCCAGGTCG AGGGTCATCT CAAATGATGG AGTTGGCAAC 
TTCATGGGAG GCAGCCTCCT GACACCCGGG GGCCCCAGAT CAGTGAAAAA TGTGGTCCAA 
GCTCAGAAGC TGGCTGATGT GGACAGCGAA CTGGCCGCCC TGCTCTTCAC ACAACCGAGA 
CCAGGGGCCC GGGGGCCCCA GGGCATCAAT CAAGAGGGGG ATGCCGTCCG CAGGCGCAAG 
CTGGAGCGCA TGAGGGCCGT GCGGCTGCGG GAATGTGGAT TGGAGCCGGG CCGCCGCGGG 
CTGGGCGCGC TGGCCCAGCA GAGCCTCCAT GCCCAGCACG CCCGAGACCT GCAGGTCATT 
GCCGCCTACC GTGAGCGCAC AAAGGCGGAG AGCATCGCCA GCGCACTGAG CACGGCCATC 
ACCACGCAGC ACACCATCCA CGCCACCCTA GGCGCTGCCG AGTTCTTCGA GTTCGCCCTG 
AGGAACCCCC ACAACGCGCA GCACATGGTG ACCATCGAGA TAGACAACCC CGAGCTCAGT 
CTCATCCTGG ATGGTCAGGA GTGGCGGTAC TTCAAGGACA TGGCTGACTT GCACACACCC 
CTGGAGGAGG ACATGTTCCA CCTGCGGGGC AGCCTGGCCC CCCAACTCTT CCTGCGACCT 
CGGGAGACTG CCCACATCCC CTTCAAGTTC CAGACTTTCT CTGTGGGCCC CTGGGCCTCG 
GCACAGGCCT CTGCTGAGCT GAGATCAGAG AAGGACATGG ATGCCGGATC ACCCTATAAG 
TCCAGCATGA TGCCCACCAA ACACGCCAAG GTCCTGTTCC GCGCGGGCAG CGGCAAGCCC 
CTGGCCGTGC TCTGCCTGAC GGTGGAGCTG CAGCCTCACG TGGTGGACCA GGTCTTCCGC 
TTCCACCACC CGGAGCTCAC CTTCCTGAAG AAAGCCATCC GCCTGCCACC CTGGCACACG 
CTTCCAGGTG CCCCTGTAGG GACTTCCAGT GAGGACCCCC CTTTCCATGT TCGATGTAGT 
GACCCGAACA TCATTTGTGA GACACAGAGT GTGGGCCCCG GGGAGCCCCG TGACGTGTTC 
CTGAAGGTGG CCAGTGGCCC TAGCCCAGAG GTCAAGGACT TCTTTGTCAC CATCTACGTA 
GACCGCTGGT TAGCAACACC TGTCCAGACC TGGCAGGTCC ACCTGCACTC GCTGCAGCGA 
GTGGACGTCT CCTGTATCAC TGGCCAGAGG ACCCGCCTGG CTCTCGTCCT CCGGGGGACG 
CAGACAGTTA GGAGAGTGCA GGCTTTCAGC TCACACCCCC AGGAGCTGAC GACGGACCCC 
AGAGGTGTGT TCGTGCTGCC TCCTCACGGG GTGCAGGACT TGCATGTGAG CGTCAGGCCC 
TGCAGGCCGG GCAGCCGCTT CGCGCACCTC CACCTGGTTG ACGTGGACGC CCACCAGCTG 
GTGGCCTCGT GGCTCCTGTG TCTCTCCTGC CGGCCGCCTC TCATCTCCAA GGCCTTCGAG 
ATAAAGCTGC CCGCGGGGAG CGGGAGGGGC GCCAATAAGC GCATCACCTA CACCAATCCC 
TACCCCTCGC GGAGGGCCTA CCGCCTGCAC AGCGACCACC CCCACCTGCT GCAGTTCAAG 
GAGGACGCCT TCCAGGTCGG GGGAGGTGAG ACCTATACCA TCGGCCTGCG GTTTGTGTCC 
CGTCAGAGAG CCGGGGAGGA AGAGATCCTG ATTTACATCA ACGACCACGA AGACAAAAAC 
GAGGAGACCT TTTGCGTGAA GGTCATCTAC CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPHP4 ( XM_015466842.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015322328.1 Bos taurus nephronophthisis 4 (NPHP4), transcript variant X3, mRNA.

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CloneID	OBa27367
Clone ID Related Accession (Same CDS sequence)	XM_015475180.1 , XM_015466842.1 , XM_015466845.1 , XM_024976773.1 , XM_005217172.4 , XM_015475179.1 , XM_015466844.1 , XM_015466846.1 , XM_005217172.3 , XM_010813390.2 , XM_024976775.1 , XM_024976776.1 , XM_005217171.3 , XM_024976772.1 , XM_010813390.3 , XM_015466843.1 , XM_005217171.4 , XM_015475180.2 , XM_024976774.1
Accession Version	XM_015466842.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4296bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	nephrocystin-4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646324.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACGACT GGCACAGGAT CTTTGACCAA AACCTGCTGG TCCCTCCGCA CCCACAGAGA 
GCACGCCAGC CTCTGAAGGA ATCGACGGCG TTTCAGTGTG TCCTGAAGTG GCTTGATGGC 
CCGCTAATTA AGCAGGGGGT GCTGGAGCTG CTATCGGAGG TGGGCTGCCA CCTGCGCGTG 
TCTCTCTTCG ATGTCACCTA TCGGCACTTC TTCGGGAGGA CGTGGAGGAG CTCGCCGAGG 
CCTGCAGAGC CGCTCACCAG GCAGCCGGCC AGGATCGTCT TCAATGAGCC CTTGTATTTT 
CACACGTCCT TGAACCACCC TCATATTGTG GCCGTGGTGG AGGTGGTCGT TGAAGGCAGA 
AGACGAGACG GGACCCTCCA GACTCTGTCC TGTGGGTTTG GAATTCTTCG GATCTTCAGC 
CACAAGCCGG AATCTCCAAC TTCCACTTCC CAGGACAAAC GGCTGAGACT GTATCATGGG 
AGCCCGCGAG CCCTCCTCCA CCCGCTCCTT CAGGACCCCA TAGAACAGAA CAAGCACATG 
ACCCTCATTG AGAACTGCAG CCTGCAGTAC ACGCTGAGGC CACACCCGCT CCTGGAGCCC 
GTGTTCCACC TTCTTCCCGA GAACTTGCTG GTGTCCGGTC TGCAGCAGAT ACCTGGCCTC 
CTTCCAGCTC ACGGGGAATC AGGAGATGCC CTCCGGAAGC CCCGCCTGCA GAAGCCTCTG 
ACGTGCTACC TGGATGATCT GTTCTTCACG CTGTATCCCT CGCTGGAGAA GTTCGAGGAG 
GAGCTTCTGG AACTCGTGAT CAGCGATTGC TTCCGGGAGG CGGGCAGCCC GCTGGACGTC 
GGCACCCTGG AGATCCTGGA GCGGCGCCTG TGCGTGGGCG TGCACAATGG GTTGGGCTTC 
GTGCAGCGGC CGCAGGTCCT CGTGCTGGTG CCCGAGCTGG AGGTGGCCTT GACACGCTCT 
GCCAGCTTCA GCCGGAAGGT CGGCTCCTCT TCCAAGGCCA GCTCTGGAAA CCAGGCGCTG 
GTCTTGAGAA GCCGTCTCCG ACTCCCTGAG ATGGTCCGTC ACCCCGCGTT CGCCATTGTC 
TTCCAGCTGG AGTACGTGTT TAACAGCCCC TCGGGGGCAG ACAGCAATGC AGCTTCACTC 
TCCTCCTTGT CCAGCCTGGC ATGTATGCAC ATGGTTCGCT GGGCTGTCTG GAACCCCTCG 
CTGGAAGCCA GCTCCGGGCA GGTGATGTTA CCTCTCCAGG GTGGCATCCA GTCCAACCCT 
TCGCACTGTC TGGTCTATAA GGTACCCCCA GCCAGCATAA GCTCCGAAGA GGTGAAGCAG 
GTGGAGTCAG GGACGGTCCA GTTCCAGTAC TCGCTGAGCC CTGAAGAACA CCCAGGCACA 
CCCACAGCGA ATGCAAGCAG CCCCAAGGCA GAGCGGCGGT CTTTTAGGAA GCCACCCGTG 
TCCCCTTCAA GTCCACCAGC ATCGCCGTCC CAGGGGCTCA CCGCTGCCCA GGACTCACCC 
ATGGGACCAG GGCTGTCACT TTCTCAGCTG GCGGCTTCCC CACCATCCCC AGCTCCCCGC 
CGCCGCTCGG CCAAACCTTC GTCACAGCCA CCTGGGAGCC CTGTGCCCTC CCTGGTCCCA 
GGGGAGTTCC CCTTGGAGGG CAGGATCTCT CACCTGGAGG CTGACCTGAG CCAGACAGCC 
TTAGCCCTGG GAACGTCCGC TGCTGACCAC TTGCAGGAAC TGCCTTTCAC ACCCGTGCAC 
GCCCCGATCA TTGCAGGAAC CCAGACCAGG AGCTCTGGAG GGAAGCTGTC ACGGGCATCC 
CTGGCGCTGC TGCAGTCCGC TGGCTTTCCC GAGATTCTGG ACGCCAACAA GCAACCAGCT 
GAGGCTGTGA GTCCTACAGA CCCCGTGAAG TTCAGCCCTC AGAAGGAAGA ATCAGACTGT 
CTACAAGGCA ATGAGATAGT GTTGCAATTT CTTGCCTTTA GCAGGGTGCC CCAGGATGAG 
CAGGCAGCGC CATGGCCACA GACTGTGTAC TTCACCTTCC AATTCTACCG CTTCCCACCC 
GAGACCACGC CACGGCTGCA GCTCGTGGAG CTGGCCGATG CTGGGAAGAC CAGCTCTGCC 
TCCCTGTCGC ACATCCTCGC CCAGATCCAG AAGGATGGCT CCTTTGATGC GGGGTCTCCG 
GGCTTTCAGC GGAAGTACCT GGTGGACCCC GGGTTCCTGA GACCCGGGGA GCAGCGCTGG 
TTCGCCCGCT ACCTGGCCAT GCAGACCCTG CAGATCGACG TCTGGGATGG GGACTCCCTG 
CTTCTCATTG GATCTGCTGC CGTTCAGCTG AAGCACCTTC TCCGCCAGGG GCGGCCGGCC 
GTGCAGGTCT CGCATGAGCT GGAGGTTGTG GCGACTGAAT ACGAGCAGGA CGCCATGGTG 
GTGAGTGGAG ACATGACCAA GTTTGGCTGC GTCAGACCCA TCGACGTCCA CACGGTGGTG 
AAGGGCCAGC TGCACTTGAC CTTGGCCAAC GTGGGTCACT TGTGTGAACA GAGAGTAAGG 
AGTCCCAGTT CCCTGCCACC TTCCAGGTCG AGGGTCATCT CAAATGATGG AGTTGGCAAC 
TTCATGGGAG GCAGCCTCCT GACACCCGGG GGCCCCAGAT CAGTGAAAAA TGTGGTCCAA 
GCTCAGAAGC TGGCTGATGT GGACAGCGAA CTGGCCGCCC TGCTCTTCAC ACAACCGAGA 
CCAGGGGCCC GGGGGCCCCA GGGCATCAAT CAAGAGGGGG ATGCCGTCCG CAGGCGCAAG 
CTGGAGCGCA TGAGGGCCGT GCGGCTGCGG GAATGTGGAT TGGAGCCGGG CCGCCGCGGG 
CTGGGCGCGC TGGCCCAGCA GAGCCTCCAT GCCCAGCACG CCCGAGACCT GCAGGTCATT 
GCCGCCTACC GTGAGCGCAC AAAGGCGGAG AGCATCGCCA GCGCACTGAG CACGGCCATC 
ACCACGCAGC ACACCATCCA CGCCACCCTA GGCGCTGCCG AGTTCTTCGA GTTCGCCCTG 
AGGAACCCCC ACAACGCGCA GCACATGGTG ACCATCGAGA TAGACAACCC CGAGCTCAGT 
CTCATCCTGG ATGGTCAGGA GTGGCGGTAC TTCAAGGACA TGGCTGACTT GCACACACCC 
CTGGAGGAGG ACATGTTCCA CCTGCGGGGC AGCCTGGCCC CCCAACTCTT CCTGCGACCT 
CGGGAGACTG CCCACATCCC CTTCAAGTTC CAGACTTTCT CTGTGGGCCC CTGGGCCTCG 
GCACAGGCCT CTGCTGAGCT GAGATCAGAG AAGGACATGG ATGCCGGATC ACCCTATAAG 
TCCAGCATGA TGCCCACCAA ACACGCCAAG GTCCTGTTCC GCGCGGGCAG CGGCAAGCCC 
CTGGCCGTGC TCTGCCTGAC GGTGGAGCTG CAGCCTCACG TGGTGGACCA GGTCTTCCGC 
TTCCACCACC CGGAGCTCAC CTTCCTGAAG AAAGCCATCC GCCTGCCACC CTGGCACACG 
CTTCCAGGTG CCCCTGTAGG GACTTCCAGT GAGGACCCCC CTTTCCATGT TCGATGTAGT 
GACCCGAACA TCATTTGTGA GACACAGAGT GTGGGCCCCG GGGAGCCCCG TGACGTGTTC 
CTGAAGGTGG CCAGTGGCCC TAGCCCAGAG GTCAAGGACT TCTTTGTCAC CATCTACGTA 
GACCGCTGGT TAGCAACACC TGTCCAGACC TGGCAGGTCC ACCTGCACTC GCTGCAGCGA 
GTGGACGTCT CCTGTATCAC TGGCCAGAGG ACCCGCCTGG CTCTCGTCCT CCGGGGGACG 
CAGACAGTTA GGAGAGTGCA GGCTTTCAGC TCACACCCCC AGGAGCTGAC GACGGACCCC 
AGAGGTGTGT TCGTGCTGCC TCCTCACGGG GTGCAGGACT TGCATGTGAG CGTCAGGCCC 
TGCAGGCCGG GCAGCCGCTT CGCGCACCTC CACCTGGTTG ACGTGGACGC CCACCAGCTG 
GTGGCCTCGT GGCTCCTGTG TCTCTCCTGC CGGCCGCCTC TCATCTCCAA GGCCTTCGAG 
ATAAAGCTGC CCGCGGGGAG CGGGAGGGGC GCCAATAAGC GCATCACCTA CACCAATCCC 
TACCCCTCGC GGAGGGCCTA CCGCCTGCAC AGCGACCACC CCCACCTGCT GCAGTTCAAG 
GAGGACGCCT TCCAGGTCGG GGGAGGTGAG ACCTATACCA TCGGCCTGCG GTTTGTGTCC 
CGTCAGAGAG CCGGGGAGGA AGAGATCCTG ATTTACATCA ACGACCACGA AGACAAAAAC 
GAGGAGACCT TTTGCGTGAA GGTCATCTAC CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015322328.1
CDS	586..4881
Translation	MHDWHRIFDQNLLVPPHPQRARQPLKESTAFQCVLKWLDGPLIKQGVLELLSEVGCHLRVSLFDVTYRHFFGRTWRSSPRPAEPLTRQPARIVFNEPLYFHTSLNHPHIVAVVEVVVEGRRRDGTLQTLSCGFGILRIFSHKPESPTSTSQDKRLRLYHGSPRALLHPLLQDPIEQNKHMTLIENCSLQYTLRPHPLLEPVFHLLPENLLVSGLQQIPGLLPAHGESGDALRKPRLQKPLTCYLDDLFFTLYPSLEKFEEELLELVISDCFREAGSPLDVGTLEILERRLCVGVHNGLGFVQRPQVLVLVPELEVALTRSASFSRKVGSSSKASSGNQALVLRSRLRLPEMVRHPAFAIVFQLEYVFNSPSGADSNAASLSSLSSLACMHMVRWAVWNPSLEASSGQVMLPLQGGIQSNPSHCLVYKVPPASISSEEVKQVESGTVQFQYSLSPEEHPGTPTANASSPKAERRSFRKPPVSPSSPPASPSQGLTAAQDSPMGPGLSLSQLAASPPSPAPRRRSAKPSSQPPGSPVPSLVPGEFPLEGRISHLEADLSQTALALGTSAADHLQELPFTPVHAPIIAGTQTRSSGGKLSRASLALLQSAGFPEILDANKQPAEAVSPTDPVKFSPQKEESDCLQGNEIVLQFLAFSRVPQDEQAAPWPQTVYFTFQFYRFPPETTPRLQLVELADAGKTSSASLSHILAQIQKDGSFDAGSPGFQRKYLVDPGFLRPGEQRWFARYLAMQTLQIDVWDGDSLLLIGSAAVQLKHLLRQGRPAVQVSHELEVVATEYEQDAMVVSGDMTKFGCVRPIDVHTVVKGQLHLTLANVGHLCEQRVRSPSSLPPSRSRVISNDGVGNFMGGSLLTPGGPRSVKNVVQAQKLADVDSELAALLFTQPRPGARGPQGINQEGDAVRRRKLERMRAVRLRECGLEPGRRGLGALAQQSLHAQHARDLQVIAAYRERTKAESIASALSTAITTQHTIHATLGAAEFFEFALRNPHNAQHMVTIEIDNPELSLILDGQEWRYFKDMADLHTPLEEDMFHLRGSLAPQLFLRPRETAHIPFKFQTFSVGPWASAQASAELRSEKDMDAGSPYKSSMMPTKHAKVLFRAGSGKPLAVLCLTVELQPHVVDQVFRFHHPELTFLKKAIRLPPWHTLPGAPVGTSSEDPPFHVRCSDPNIICETQSVGPGEPRDVFLKVASGPSPEVKDFFVTIYVDRWLATPVQTWQVHLHSLQRVDVSCITGQRTRLALVLRGTQTVRRVQAFSSHPQELTTDPRGVFVLPPHGVQDLHVSVRPCRPGSRFAHLHLVDVDAHQLVASWLLCLSCRPPLISKAFEIKLPAGSGRGANKRITYTNPYPSRRAYRLHSDHPHLLQFKEDAFQVGGGETYTIGLRFVSRQRAGEEEILIYINDHEDKNEETFCVKVIYQ

Target ORF information:

RefSeq Version	XM_015466842.1
Organism	Bos taurus(cattle)
Definition	Bos taurus nephronophthisis 4 (NPHP4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015466842.1

ORF Insert Sequence:

ATGCACGACT GGCACAGGAT CTTTGACCAA AACCTGCTGG TCCCTCCGCA CCCACAGAGA 
GCACGCCAGC CTCTGAAGGA ATCGACGGCG TTTCAGTGTG TCCTGAAGTG GCTTGATGGC 
CCGCTAATTA AGCAGGGGGT GCTGGAGCTG CTATCGGAGG TGGGCTGCCA CCTGCGCGTG 
TCTCTCTTCG ATGTCACCTA TCGGCACTTC TTCGGGAGGA CGTGGAGGAG CTCGCCGAGG 
CCTGCAGAGC CGCTCACCAG GCAGCCGGCC AGGATCGTCT TCAATGAGCC CTTGTATTTT 
CACACGTCCT TGAACCACCC TCATATTGTG GCCGTGGTGG AGGTGGTCGT TGAAGGCAGA 
AGACGAGACG GGACCCTCCA GACTCTGTCC TGTGGGTTTG GAATTCTTCG GATCTTCAGC 
CACAAGCCGG AATCTCCAAC TTCCACTTCC CAGGACAAAC GGCTGAGACT GTATCATGGG 
AGCCCGCGAG CCCTCCTCCA CCCGCTCCTT CAGGACCCCA TAGAACAGAA CAAGCACATG 
ACCCTCATTG AGAACTGCAG CCTGCAGTAC ACGCTGAGGC CACACCCGCT CCTGGAGCCC 
GTGTTCCACC TTCTTCCCGA GAACTTGCTG GTGTCCGGTC TGCAGCAGAT ACCTGGCCTC 
CTTCCAGCTC ACGGGGAATC AGGAGATGCC CTCCGGAAGC CCCGCCTGCA GAAGCCTCTG 
ACGTGCTACC TGGATGATCT GTTCTTCACG CTGTATCCCT CGCTGGAGAA GTTCGAGGAG 
GAGCTTCTGG AACTCGTGAT CAGCGATTGC TTCCGGGAGG CGGGCAGCCC GCTGGACGTC 
GGCACCCTGG AGATCCTGGA GCGGCGCCTG TGCGTGGGCG TGCACAATGG GTTGGGCTTC 
GTGCAGCGGC CGCAGGTCCT CGTGCTGGTG CCCGAGCTGG AGGTGGCCTT GACACGCTCT 
GCCAGCTTCA GCCGGAAGGT CGGCTCCTCT TCCAAGGCCA GCTCTGGAAA CCAGGCGCTG 
GTCTTGAGAA GCCGTCTCCG ACTCCCTGAG ATGGTCCGTC ACCCCGCGTT CGCCATTGTC 
TTCCAGCTGG AGTACGTGTT TAACAGCCCC TCGGGGGCAG ACAGCAATGC AGCTTCACTC 
TCCTCCTTGT CCAGCCTGGC ATGTATGCAC ATGGTTCGCT GGGCTGTCTG GAACCCCTCG 
CTGGAAGCCA GCTCCGGGCA GGTGATGTTA CCTCTCCAGG GTGGCATCCA GTCCAACCCT 
TCGCACTGTC TGGTCTATAA GGTACCCCCA GCCAGCATAA GCTCCGAAGA GGTGAAGCAG 
GTGGAGTCAG GGACGGTCCA GTTCCAGTAC TCGCTGAGCC CTGAAGAACA CCCAGGCACA 
CCCACAGCGA ATGCAAGCAG CCCCAAGGCA GAGCGGCGGT CTTTTAGGAA GCCACCCGTG 
TCCCCTTCAA GTCCACCAGC ATCGCCGTCC CAGGGGCTCA CCGCTGCCCA GGACTCACCC 
ATGGGACCAG GGCTGTCACT TTCTCAGCTG GCGGCTTCCC CACCATCCCC AGCTCCCCGC 
CGCCGCTCGG CCAAACCTTC GTCACAGCCA CCTGGGAGCC CTGTGCCCTC CCTGGTCCCA 
GGGGAGTTCC CCTTGGAGGG CAGGATCTCT CACCTGGAGG CTGACCTGAG CCAGACAGCC 
TTAGCCCTGG GAACGTCCGC TGCTGACCAC TTGCAGGAAC TGCCTTTCAC ACCCGTGCAC 
GCCCCGATCA TTGCAGGAAC CCAGACCAGG AGCTCTGGAG GGAAGCTGTC ACGGGCATCC 
CTGGCGCTGC TGCAGTCCGC TGGCTTTCCC GAGATTCTGG ACGCCAACAA GCAACCAGCT 
GAGGCTGTGA GTCCTACAGA CCCCGTGAAG TTCAGCCCTC AGAAGGAAGA ATCAGACTGT 
CTACAAGGCA ATGAGATAGT GTTGCAATTT CTTGCCTTTA GCAGGGTGCC CCAGGATGAG 
CAGGCAGCGC CATGGCCACA GACTGTGTAC TTCACCTTCC AATTCTACCG CTTCCCACCC 
GAGACCACGC CACGGCTGCA GCTCGTGGAG CTGGCCGATG CTGGGAAGAC CAGCTCTGCC 
TCCCTGTCGC ACATCCTCGC CCAGATCCAG AAGGATGGCT CCTTTGATGC GGGGTCTCCG 
GGCTTTCAGC GGAAGTACCT GGTGGACCCC GGGTTCCTGA GACCCGGGGA GCAGCGCTGG 
TTCGCCCGCT ACCTGGCCAT GCAGACCCTG CAGATCGACG TCTGGGATGG GGACTCCCTG 
CTTCTCATTG GATCTGCTGC CGTTCAGCTG AAGCACCTTC TCCGCCAGGG GCGGCCGGCC 
GTGCAGGTCT CGCATGAGCT GGAGGTTGTG GCGACTGAAT ACGAGCAGGA CGCCATGGTG 
GTGAGTGGAG ACATGACCAA GTTTGGCTGC GTCAGACCCA TCGACGTCCA CACGGTGGTG 
AAGGGCCAGC TGCACTTGAC CTTGGCCAAC GTGGGTCACT TGTGTGAACA GAGAGTAAGG 
AGTCCCAGTT CCCTGCCACC TTCCAGGTCG AGGGTCATCT CAAATGATGG AGTTGGCAAC 
TTCATGGGAG GCAGCCTCCT GACACCCGGG GGCCCCAGAT CAGTGAAAAA TGTGGTCCAA 
GCTCAGAAGC TGGCTGATGT GGACAGCGAA CTGGCCGCCC TGCTCTTCAC ACAACCGAGA 
CCAGGGGCCC GGGGGCCCCA GGGCATCAAT CAAGAGGGGG ATGCCGTCCG CAGGCGCAAG 
CTGGAGCGCA TGAGGGCCGT GCGGCTGCGG GAATGTGGAT TGGAGCCGGG CCGCCGCGGG 
CTGGGCGCGC TGGCCCAGCA GAGCCTCCAT GCCCAGCACG CCCGAGACCT GCAGGTCATT 
GCCGCCTACC GTGAGCGCAC AAAGGCGGAG AGCATCGCCA GCGCACTGAG CACGGCCATC 
ACCACGCAGC ACACCATCCA CGCCACCCTA GGCGCTGCCG AGTTCTTCGA GTTCGCCCTG 
AGGAACCCCC ACAACGCGCA GCACATGGTG ACCATCGAGA TAGACAACCC CGAGCTCAGT 
CTCATCCTGG ATGGTCAGGA GTGGCGGTAC TTCAAGGACA TGGCTGACTT GCACACACCC 
CTGGAGGAGG ACATGTTCCA CCTGCGGGGC AGCCTGGCCC CCCAACTCTT CCTGCGACCT 
CGGGAGACTG CCCACATCCC CTTCAAGTTC CAGACTTTCT CTGTGGGCCC CTGGGCCTCG 
GCACAGGCCT CTGCTGAGCT GAGATCAGAG AAGGACATGG ATGCCGGATC ACCCTATAAG 
TCCAGCATGA TGCCCACCAA ACACGCCAAG GTCCTGTTCC GCGCGGGCAG CGGCAAGCCC 
CTGGCCGTGC TCTGCCTGAC GGTGGAGCTG CAGCCTCACG TGGTGGACCA GGTCTTCCGC 
TTCCACCACC CGGAGCTCAC CTTCCTGAAG AAAGCCATCC GCCTGCCACC CTGGCACACG 
CTTCCAGGTG CCCCTGTAGG GACTTCCAGT GAGGACCCCC CTTTCCATGT TCGATGTAGT 
GACCCGAACA TCATTTGTGA GACACAGAGT GTGGGCCCCG GGGAGCCCCG TGACGTGTTC 
CTGAAGGTGG CCAGTGGCCC TAGCCCAGAG GTCAAGGACT TCTTTGTCAC CATCTACGTA 
GACCGCTGGT TAGCAACACC TGTCCAGACC TGGCAGGTCC ACCTGCACTC GCTGCAGCGA 
GTGGACGTCT CCTGTATCAC TGGCCAGAGG ACCCGCCTGG CTCTCGTCCT CCGGGGGACG 
CAGACAGTTA GGAGAGTGCA GGCTTTCAGC TCACACCCCC AGGAGCTGAC GACGGACCCC 
AGAGGTGTGT TCGTGCTGCC TCCTCACGGG GTGCAGGACT TGCATGTGAG CGTCAGGCCC 
TGCAGGCCGG GCAGCCGCTT CGCGCACCTC CACCTGGTTG ACGTGGACGC CCACCAGCTG 
GTGGCCTCGT GGCTCCTGTG TCTCTCCTGC CGGCCGCCTC TCATCTCCAA GGCCTTCGAG 
ATAAAGCTGC CCGCGGGGAG CGGGAGGGGC GCCAATAAGC GCATCACCTA CACCAATCCC 
TACCCCTCGC GGAGGGCCTA CCGCCTGCAC AGCGACCACC CCCACCTGCT GCAGTTCAAG 
GAGGACGCCT TCCAGGTCGG GGGAGGTGAG ACCTATACCA TCGGCCTGCG GTTTGTGTCC 
CGTCAGAGAG CCGGGGAGGA AGAGATCCTG ATTTACATCA ACGACCACGA AGACAAAAAC 
GAGGAGACCT TTTGCGTGAA GGTCATCTAC CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPHP4 ( XM_015466845.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015322331.1 Bos taurus nephronophthisis 4 (NPHP4), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBa27367
Clone ID Related Accession (Same CDS sequence)	XM_015475180.1 , XM_015466842.1 , XM_015466845.1 , XM_024976773.1 , XM_005217172.4 , XM_015475179.1 , XM_015466844.1 , XM_015466846.1 , XM_005217172.3 , XM_010813390.2 , XM_024976775.1 , XM_024976776.1 , XM_005217171.3 , XM_024976772.1 , XM_010813390.3 , XM_015466843.1 , XM_005217171.4 , XM_015475180.2 , XM_024976774.1
Accession Version	XM_015466845.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4296bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	nephrocystin-4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646324.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACGACT GGCACAGGAT CTTTGACCAA AACCTGCTGG TCCCTCCGCA CCCACAGAGA 
GCACGCCAGC CTCTGAAGGA ATCGACGGCG TTTCAGTGTG TCCTGAAGTG GCTTGATGGC 
CCGCTAATTA AGCAGGGGGT GCTGGAGCTG CTATCGGAGG TGGGCTGCCA CCTGCGCGTG 
TCTCTCTTCG ATGTCACCTA TCGGCACTTC TTCGGGAGGA CGTGGAGGAG CTCGCCGAGG 
CCTGCAGAGC CGCTCACCAG GCAGCCGGCC AGGATCGTCT TCAATGAGCC CTTGTATTTT 
CACACGTCCT TGAACCACCC TCATATTGTG GCCGTGGTGG AGGTGGTCGT TGAAGGCAGA 
AGACGAGACG GGACCCTCCA GACTCTGTCC TGTGGGTTTG GAATTCTTCG GATCTTCAGC 
CACAAGCCGG AATCTCCAAC TTCCACTTCC CAGGACAAAC GGCTGAGACT GTATCATGGG 
AGCCCGCGAG CCCTCCTCCA CCCGCTCCTT CAGGACCCCA TAGAACAGAA CAAGCACATG 
ACCCTCATTG AGAACTGCAG CCTGCAGTAC ACGCTGAGGC CACACCCGCT CCTGGAGCCC 
GTGTTCCACC TTCTTCCCGA GAACTTGCTG GTGTCCGGTC TGCAGCAGAT ACCTGGCCTC 
CTTCCAGCTC ACGGGGAATC AGGAGATGCC CTCCGGAAGC CCCGCCTGCA GAAGCCTCTG 
ACGTGCTACC TGGATGATCT GTTCTTCACG CTGTATCCCT CGCTGGAGAA GTTCGAGGAG 
GAGCTTCTGG AACTCGTGAT CAGCGATTGC TTCCGGGAGG CGGGCAGCCC GCTGGACGTC 
GGCACCCTGG AGATCCTGGA GCGGCGCCTG TGCGTGGGCG TGCACAATGG GTTGGGCTTC 
GTGCAGCGGC CGCAGGTCCT CGTGCTGGTG CCCGAGCTGG AGGTGGCCTT GACACGCTCT 
GCCAGCTTCA GCCGGAAGGT CGGCTCCTCT TCCAAGGCCA GCTCTGGAAA CCAGGCGCTG 
GTCTTGAGAA GCCGTCTCCG ACTCCCTGAG ATGGTCCGTC ACCCCGCGTT CGCCATTGTC 
TTCCAGCTGG AGTACGTGTT TAACAGCCCC TCGGGGGCAG ACAGCAATGC AGCTTCACTC 
TCCTCCTTGT CCAGCCTGGC ATGTATGCAC ATGGTTCGCT GGGCTGTCTG GAACCCCTCG 
CTGGAAGCCA GCTCCGGGCA GGTGATGTTA CCTCTCCAGG GTGGCATCCA GTCCAACCCT 
TCGCACTGTC TGGTCTATAA GGTACCCCCA GCCAGCATAA GCTCCGAAGA GGTGAAGCAG 
GTGGAGTCAG GGACGGTCCA GTTCCAGTAC TCGCTGAGCC CTGAAGAACA CCCAGGCACA 
CCCACAGCGA ATGCAAGCAG CCCCAAGGCA GAGCGGCGGT CTTTTAGGAA GCCACCCGTG 
TCCCCTTCAA GTCCACCAGC ATCGCCGTCC CAGGGGCTCA CCGCTGCCCA GGACTCACCC 
ATGGGACCAG GGCTGTCACT TTCTCAGCTG GCGGCTTCCC CACCATCCCC AGCTCCCCGC 
CGCCGCTCGG CCAAACCTTC GTCACAGCCA CCTGGGAGCC CTGTGCCCTC CCTGGTCCCA 
GGGGAGTTCC CCTTGGAGGG CAGGATCTCT CACCTGGAGG CTGACCTGAG CCAGACAGCC 
TTAGCCCTGG GAACGTCCGC TGCTGACCAC TTGCAGGAAC TGCCTTTCAC ACCCGTGCAC 
GCCCCGATCA TTGCAGGAAC CCAGACCAGG AGCTCTGGAG GGAAGCTGTC ACGGGCATCC 
CTGGCGCTGC TGCAGTCCGC TGGCTTTCCC GAGATTCTGG ACGCCAACAA GCAACCAGCT 
GAGGCTGTGA GTCCTACAGA CCCCGTGAAG TTCAGCCCTC AGAAGGAAGA ATCAGACTGT 
CTACAAGGCA ATGAGATAGT GTTGCAATTT CTTGCCTTTA GCAGGGTGCC CCAGGATGAG 
CAGGCAGCGC CATGGCCACA GACTGTGTAC TTCACCTTCC AATTCTACCG CTTCCCACCC 
GAGACCACGC CACGGCTGCA GCTCGTGGAG CTGGCCGATG CTGGGAAGAC CAGCTCTGCC 
TCCCTGTCGC ACATCCTCGC CCAGATCCAG AAGGATGGCT CCTTTGATGC GGGGTCTCCG 
GGCTTTCAGC GGAAGTACCT GGTGGACCCC GGGTTCCTGA GACCCGGGGA GCAGCGCTGG 
TTCGCCCGCT ACCTGGCCAT GCAGACCCTG CAGATCGACG TCTGGGATGG GGACTCCCTG 
CTTCTCATTG GATCTGCTGC CGTTCAGCTG AAGCACCTTC TCCGCCAGGG GCGGCCGGCC 
GTGCAGGTCT CGCATGAGCT GGAGGTTGTG GCGACTGAAT ACGAGCAGGA CGCCATGGTG 
GTGAGTGGAG ACATGACCAA GTTTGGCTGC GTCAGACCCA TCGACGTCCA CACGGTGGTG 
AAGGGCCAGC TGCACTTGAC CTTGGCCAAC GTGGGTCACT TGTGTGAACA GAGAGTAAGG 
AGTCCCAGTT CCCTGCCACC TTCCAGGTCG AGGGTCATCT CAAATGATGG AGTTGGCAAC 
TTCATGGGAG GCAGCCTCCT GACACCCGGG GGCCCCAGAT CAGTGAAAAA TGTGGTCCAA 
GCTCAGAAGC TGGCTGATGT GGACAGCGAA CTGGCCGCCC TGCTCTTCAC ACAACCGAGA 
CCAGGGGCCC GGGGGCCCCA GGGCATCAAT CAAGAGGGGG ATGCCGTCCG CAGGCGCAAG 
CTGGAGCGCA TGAGGGCCGT GCGGCTGCGG GAATGTGGAT TGGAGCCGGG CCGCCGCGGG 
CTGGGCGCGC TGGCCCAGCA GAGCCTCCAT GCCCAGCACG CCCGAGACCT GCAGGTCATT 
GCCGCCTACC GTGAGCGCAC AAAGGCGGAG AGCATCGCCA GCGCACTGAG CACGGCCATC 
ACCACGCAGC ACACCATCCA CGCCACCCTA GGCGCTGCCG AGTTCTTCGA GTTCGCCCTG 
AGGAACCCCC ACAACGCGCA GCACATGGTG ACCATCGAGA TAGACAACCC CGAGCTCAGT 
CTCATCCTGG ATGGTCAGGA GTGGCGGTAC TTCAAGGACA TGGCTGACTT GCACACACCC 
CTGGAGGAGG ACATGTTCCA CCTGCGGGGC AGCCTGGCCC CCCAACTCTT CCTGCGACCT 
CGGGAGACTG CCCACATCCC CTTCAAGTTC CAGACTTTCT CTGTGGGCCC CTGGGCCTCG 
GCACAGGCCT CTGCTGAGCT GAGATCAGAG AAGGACATGG ATGCCGGATC ACCCTATAAG 
TCCAGCATGA TGCCCACCAA ACACGCCAAG GTCCTGTTCC GCGCGGGCAG CGGCAAGCCC 
CTGGCCGTGC TCTGCCTGAC GGTGGAGCTG CAGCCTCACG TGGTGGACCA GGTCTTCCGC 
TTCCACCACC CGGAGCTCAC CTTCCTGAAG AAAGCCATCC GCCTGCCACC CTGGCACACG 
CTTCCAGGTG CCCCTGTAGG GACTTCCAGT GAGGACCCCC CTTTCCATGT TCGATGTAGT 
GACCCGAACA TCATTTGTGA GACACAGAGT GTGGGCCCCG GGGAGCCCCG TGACGTGTTC 
CTGAAGGTGG CCAGTGGCCC TAGCCCAGAG GTCAAGGACT TCTTTGTCAC CATCTACGTA 
GACCGCTGGT TAGCAACACC TGTCCAGACC TGGCAGGTCC ACCTGCACTC GCTGCAGCGA 
GTGGACGTCT CCTGTATCAC TGGCCAGAGG ACCCGCCTGG CTCTCGTCCT CCGGGGGACG 
CAGACAGTTA GGAGAGTGCA GGCTTTCAGC TCACACCCCC AGGAGCTGAC GACGGACCCC 
AGAGGTGTGT TCGTGCTGCC TCCTCACGGG GTGCAGGACT TGCATGTGAG CGTCAGGCCC 
TGCAGGCCGG GCAGCCGCTT CGCGCACCTC CACCTGGTTG ACGTGGACGC CCACCAGCTG 
GTGGCCTCGT GGCTCCTGTG TCTCTCCTGC CGGCCGCCTC TCATCTCCAA GGCCTTCGAG 
ATAAAGCTGC CCGCGGGGAG CGGGAGGGGC GCCAATAAGC GCATCACCTA CACCAATCCC 
TACCCCTCGC GGAGGGCCTA CCGCCTGCAC AGCGACCACC CCCACCTGCT GCAGTTCAAG 
GAGGACGCCT TCCAGGTCGG GGGAGGTGAG ACCTATACCA TCGGCCTGCG GTTTGTGTCC 
CGTCAGAGAG CCGGGGAGGA AGAGATCCTG ATTTACATCA ACGACCACGA AGACAAAAAC 
GAGGAGACCT TTTGCGTGAA GGTCATCTAC CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015322331.1
CDS	1645..5940
Translation	MHDWHRIFDQNLLVPPHPQRARQPLKESTAFQCVLKWLDGPLIKQGVLELLSEVGCHLRVSLFDVTYRHFFGRTWRSSPRPAEPLTRQPARIVFNEPLYFHTSLNHPHIVAVVEVVVEGRRRDGTLQTLSCGFGILRIFSHKPESPTSTSQDKRLRLYHGSPRALLHPLLQDPIEQNKHMTLIENCSLQYTLRPHPLLEPVFHLLPENLLVSGLQQIPGLLPAHGESGDALRKPRLQKPLTCYLDDLFFTLYPSLEKFEEELLELVISDCFREAGSPLDVGTLEILERRLCVGVHNGLGFVQRPQVLVLVPELEVALTRSASFSRKVGSSSKASSGNQALVLRSRLRLPEMVRHPAFAIVFQLEYVFNSPSGADSNAASLSSLSSLACMHMVRWAVWNPSLEASSGQVMLPLQGGIQSNPSHCLVYKVPPASISSEEVKQVESGTVQFQYSLSPEEHPGTPTANASSPKAERRSFRKPPVSPSSPPASPSQGLTAAQDSPMGPGLSLSQLAASPPSPAPRRRSAKPSSQPPGSPVPSLVPGEFPLEGRISHLEADLSQTALALGTSAADHLQELPFTPVHAPIIAGTQTRSSGGKLSRASLALLQSAGFPEILDANKQPAEAVSPTDPVKFSPQKEESDCLQGNEIVLQFLAFSRVPQDEQAAPWPQTVYFTFQFYRFPPETTPRLQLVELADAGKTSSASLSHILAQIQKDGSFDAGSPGFQRKYLVDPGFLRPGEQRWFARYLAMQTLQIDVWDGDSLLLIGSAAVQLKHLLRQGRPAVQVSHELEVVATEYEQDAMVVSGDMTKFGCVRPIDVHTVVKGQLHLTLANVGHLCEQRVRSPSSLPPSRSRVISNDGVGNFMGGSLLTPGGPRSVKNVVQAQKLADVDSELAALLFTQPRPGARGPQGINQEGDAVRRRKLERMRAVRLRECGLEPGRRGLGALAQQSLHAQHARDLQVIAAYRERTKAESIASALSTAITTQHTIHATLGAAEFFEFALRNPHNAQHMVTIEIDNPELSLILDGQEWRYFKDMADLHTPLEEDMFHLRGSLAPQLFLRPRETAHIPFKFQTFSVGPWASAQASAELRSEKDMDAGSPYKSSMMPTKHAKVLFRAGSGKPLAVLCLTVELQPHVVDQVFRFHHPELTFLKKAIRLPPWHTLPGAPVGTSSEDPPFHVRCSDPNIICETQSVGPGEPRDVFLKVASGPSPEVKDFFVTIYVDRWLATPVQTWQVHLHSLQRVDVSCITGQRTRLALVLRGTQTVRRVQAFSSHPQELTTDPRGVFVLPPHGVQDLHVSVRPCRPGSRFAHLHLVDVDAHQLVASWLLCLSCRPPLISKAFEIKLPAGSGRGANKRITYTNPYPSRRAYRLHSDHPHLLQFKEDAFQVGGGETYTIGLRFVSRQRAGEEEILIYINDHEDKNEETFCVKVIYQ

Target ORF information:

RefSeq Version	XM_015466845.1
Organism	Bos taurus(cattle)
Definition	Bos taurus nephronophthisis 4 (NPHP4), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015466845.1

ORF Insert Sequence:

ATGCACGACT GGCACAGGAT CTTTGACCAA AACCTGCTGG TCCCTCCGCA CCCACAGAGA 
GCACGCCAGC CTCTGAAGGA ATCGACGGCG TTTCAGTGTG TCCTGAAGTG GCTTGATGGC 
CCGCTAATTA AGCAGGGGGT GCTGGAGCTG CTATCGGAGG TGGGCTGCCA CCTGCGCGTG 
TCTCTCTTCG ATGTCACCTA TCGGCACTTC TTCGGGAGGA CGTGGAGGAG CTCGCCGAGG 
CCTGCAGAGC CGCTCACCAG GCAGCCGGCC AGGATCGTCT TCAATGAGCC CTTGTATTTT 
CACACGTCCT TGAACCACCC TCATATTGTG GCCGTGGTGG AGGTGGTCGT TGAAGGCAGA 
AGACGAGACG GGACCCTCCA GACTCTGTCC TGTGGGTTTG GAATTCTTCG GATCTTCAGC 
CACAAGCCGG AATCTCCAAC TTCCACTTCC CAGGACAAAC GGCTGAGACT GTATCATGGG 
AGCCCGCGAG CCCTCCTCCA CCCGCTCCTT CAGGACCCCA TAGAACAGAA CAAGCACATG 
ACCCTCATTG AGAACTGCAG CCTGCAGTAC ACGCTGAGGC CACACCCGCT CCTGGAGCCC 
GTGTTCCACC TTCTTCCCGA GAACTTGCTG GTGTCCGGTC TGCAGCAGAT ACCTGGCCTC 
CTTCCAGCTC ACGGGGAATC AGGAGATGCC CTCCGGAAGC CCCGCCTGCA GAAGCCTCTG 
ACGTGCTACC TGGATGATCT GTTCTTCACG CTGTATCCCT CGCTGGAGAA GTTCGAGGAG 
GAGCTTCTGG AACTCGTGAT CAGCGATTGC TTCCGGGAGG CGGGCAGCCC GCTGGACGTC 
GGCACCCTGG AGATCCTGGA GCGGCGCCTG TGCGTGGGCG TGCACAATGG GTTGGGCTTC 
GTGCAGCGGC CGCAGGTCCT CGTGCTGGTG CCCGAGCTGG AGGTGGCCTT GACACGCTCT 
GCCAGCTTCA GCCGGAAGGT CGGCTCCTCT TCCAAGGCCA GCTCTGGAAA CCAGGCGCTG 
GTCTTGAGAA GCCGTCTCCG ACTCCCTGAG ATGGTCCGTC ACCCCGCGTT CGCCATTGTC 
TTCCAGCTGG AGTACGTGTT TAACAGCCCC TCGGGGGCAG ACAGCAATGC AGCTTCACTC 
TCCTCCTTGT CCAGCCTGGC ATGTATGCAC ATGGTTCGCT GGGCTGTCTG GAACCCCTCG 
CTGGAAGCCA GCTCCGGGCA GGTGATGTTA CCTCTCCAGG GTGGCATCCA GTCCAACCCT 
TCGCACTGTC TGGTCTATAA GGTACCCCCA GCCAGCATAA GCTCCGAAGA GGTGAAGCAG 
GTGGAGTCAG GGACGGTCCA GTTCCAGTAC TCGCTGAGCC CTGAAGAACA CCCAGGCACA 
CCCACAGCGA ATGCAAGCAG CCCCAAGGCA GAGCGGCGGT CTTTTAGGAA GCCACCCGTG 
TCCCCTTCAA GTCCACCAGC ATCGCCGTCC CAGGGGCTCA CCGCTGCCCA GGACTCACCC 
ATGGGACCAG GGCTGTCACT TTCTCAGCTG GCGGCTTCCC CACCATCCCC AGCTCCCCGC 
CGCCGCTCGG CCAAACCTTC GTCACAGCCA CCTGGGAGCC CTGTGCCCTC CCTGGTCCCA 
GGGGAGTTCC CCTTGGAGGG CAGGATCTCT CACCTGGAGG CTGACCTGAG CCAGACAGCC 
TTAGCCCTGG GAACGTCCGC TGCTGACCAC TTGCAGGAAC TGCCTTTCAC ACCCGTGCAC 
GCCCCGATCA TTGCAGGAAC CCAGACCAGG AGCTCTGGAG GGAAGCTGTC ACGGGCATCC 
CTGGCGCTGC TGCAGTCCGC TGGCTTTCCC GAGATTCTGG ACGCCAACAA GCAACCAGCT 
GAGGCTGTGA GTCCTACAGA CCCCGTGAAG TTCAGCCCTC AGAAGGAAGA ATCAGACTGT 
CTACAAGGCA ATGAGATAGT GTTGCAATTT CTTGCCTTTA GCAGGGTGCC CCAGGATGAG 
CAGGCAGCGC CATGGCCACA GACTGTGTAC TTCACCTTCC AATTCTACCG CTTCCCACCC 
GAGACCACGC CACGGCTGCA GCTCGTGGAG CTGGCCGATG CTGGGAAGAC CAGCTCTGCC 
TCCCTGTCGC ACATCCTCGC CCAGATCCAG AAGGATGGCT CCTTTGATGC GGGGTCTCCG 
GGCTTTCAGC GGAAGTACCT GGTGGACCCC GGGTTCCTGA GACCCGGGGA GCAGCGCTGG 
TTCGCCCGCT ACCTGGCCAT GCAGACCCTG CAGATCGACG TCTGGGATGG GGACTCCCTG 
CTTCTCATTG GATCTGCTGC CGTTCAGCTG AAGCACCTTC TCCGCCAGGG GCGGCCGGCC 
GTGCAGGTCT CGCATGAGCT GGAGGTTGTG GCGACTGAAT ACGAGCAGGA CGCCATGGTG 
GTGAGTGGAG ACATGACCAA GTTTGGCTGC GTCAGACCCA TCGACGTCCA CACGGTGGTG 
AAGGGCCAGC TGCACTTGAC CTTGGCCAAC GTGGGTCACT TGTGTGAACA GAGAGTAAGG 
AGTCCCAGTT CCCTGCCACC TTCCAGGTCG AGGGTCATCT CAAATGATGG AGTTGGCAAC 
TTCATGGGAG GCAGCCTCCT GACACCCGGG GGCCCCAGAT CAGTGAAAAA TGTGGTCCAA 
GCTCAGAAGC TGGCTGATGT GGACAGCGAA CTGGCCGCCC TGCTCTTCAC ACAACCGAGA 
CCAGGGGCCC GGGGGCCCCA GGGCATCAAT CAAGAGGGGG ATGCCGTCCG CAGGCGCAAG 
CTGGAGCGCA TGAGGGCCGT GCGGCTGCGG GAATGTGGAT TGGAGCCGGG CCGCCGCGGG 
CTGGGCGCGC TGGCCCAGCA GAGCCTCCAT GCCCAGCACG CCCGAGACCT GCAGGTCATT 
GCCGCCTACC GTGAGCGCAC AAAGGCGGAG AGCATCGCCA GCGCACTGAG CACGGCCATC 
ACCACGCAGC ACACCATCCA CGCCACCCTA GGCGCTGCCG AGTTCTTCGA GTTCGCCCTG 
AGGAACCCCC ACAACGCGCA GCACATGGTG ACCATCGAGA TAGACAACCC CGAGCTCAGT 
CTCATCCTGG ATGGTCAGGA GTGGCGGTAC TTCAAGGACA TGGCTGACTT GCACACACCC 
CTGGAGGAGG ACATGTTCCA CCTGCGGGGC AGCCTGGCCC CCCAACTCTT CCTGCGACCT 
CGGGAGACTG CCCACATCCC CTTCAAGTTC CAGACTTTCT CTGTGGGCCC CTGGGCCTCG 
GCACAGGCCT CTGCTGAGCT GAGATCAGAG AAGGACATGG ATGCCGGATC ACCCTATAAG 
TCCAGCATGA TGCCCACCAA ACACGCCAAG GTCCTGTTCC GCGCGGGCAG CGGCAAGCCC 
CTGGCCGTGC TCTGCCTGAC GGTGGAGCTG CAGCCTCACG TGGTGGACCA GGTCTTCCGC 
TTCCACCACC CGGAGCTCAC CTTCCTGAAG AAAGCCATCC GCCTGCCACC CTGGCACACG 
CTTCCAGGTG CCCCTGTAGG GACTTCCAGT GAGGACCCCC CTTTCCATGT TCGATGTAGT 
GACCCGAACA TCATTTGTGA GACACAGAGT GTGGGCCCCG GGGAGCCCCG TGACGTGTTC 
CTGAAGGTGG CCAGTGGCCC TAGCCCAGAG GTCAAGGACT TCTTTGTCAC CATCTACGTA 
GACCGCTGGT TAGCAACACC TGTCCAGACC TGGCAGGTCC ACCTGCACTC GCTGCAGCGA 
GTGGACGTCT CCTGTATCAC TGGCCAGAGG ACCCGCCTGG CTCTCGTCCT CCGGGGGACG 
CAGACAGTTA GGAGAGTGCA GGCTTTCAGC TCACACCCCC AGGAGCTGAC GACGGACCCC 
AGAGGTGTGT TCGTGCTGCC TCCTCACGGG GTGCAGGACT TGCATGTGAG CGTCAGGCCC 
TGCAGGCCGG GCAGCCGCTT CGCGCACCTC CACCTGGTTG ACGTGGACGC CCACCAGCTG 
GTGGCCTCGT GGCTCCTGTG TCTCTCCTGC CGGCCGCCTC TCATCTCCAA GGCCTTCGAG 
ATAAAGCTGC CCGCGGGGAG CGGGAGGGGC GCCAATAAGC GCATCACCTA CACCAATCCC 
TACCCCTCGC GGAGGGCCTA CCGCCTGCAC AGCGACCACC CCCACCTGCT GCAGTTCAAG 
GAGGACGCCT TCCAGGTCGG GGGAGGTGAG ACCTATACCA TCGGCCTGCG GTTTGTGTCC 
CGTCAGAGAG CCGGGGAGGA AGAGATCCTG ATTTACATCA ACGACCACGA AGACAAAAAC 
GAGGAGACCT TTTGCGTGAA GGTCATCTAC CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPHP4 ( XM_024976773.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832541.1 Bos taurus nephrocystin 4 (NPHP4), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa27367
Clone ID Related Accession (Same CDS sequence)	XM_015475180.1 , XM_015466842.1 , XM_015466845.1 , XM_024976773.1 , XM_005217172.4 , XM_015475179.1 , XM_015466844.1 , XM_015466846.1 , XM_005217172.3 , XM_010813390.2 , XM_024976775.1 , XM_024976776.1 , XM_005217171.3 , XM_024976772.1 , XM_010813390.3 , XM_015466843.1 , XM_005217171.4 , XM_015475180.2 , XM_024976774.1
Accession Version	XM_024976773.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4296bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	nephrocystin-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACGACT GGCACAGGAT CTTTGACCAA AACCTGCTGG TCCCTCCGCA CCCACAGAGA 
GCACGCCAGC CTCTGAAGGA ATCGACGGCG TTTCAGTGTG TCCTGAAGTG GCTTGATGGC 
CCGCTAATTA AGCAGGGGGT GCTGGAGCTG CTATCGGAGG TGGGCTGCCA CCTGCGCGTG 
TCTCTCTTCG ATGTCACCTA TCGGCACTTC TTCGGGAGGA CGTGGAGGAG CTCGCCGAGG 
CCTGCAGAGC CGCTCACCAG GCAGCCGGCC AGGATCGTCT TCAATGAGCC CTTGTATTTT 
CACACGTCCT TGAACCACCC TCATATTGTG GCCGTGGTGG AGGTGGTCGT TGAAGGCAGA 
AGACGAGACG GGACCCTCCA GACTCTGTCC TGTGGGTTTG GAATTCTTCG GATCTTCAGC 
CACAAGCCGG AATCTCCAAC TTCCACTTCC CAGGACAAAC GGCTGAGACT GTATCATGGG 
AGCCCGCGAG CCCTCCTCCA CCCGCTCCTT CAGGACCCCA TAGAACAGAA CAAGCACATG 
ACCCTCATTG AGAACTGCAG CCTGCAGTAC ACGCTGAGGC CACACCCGCT CCTGGAGCCC 
GTGTTCCACC TTCTTCCCGA GAACTTGCTG GTGTCCGGTC TGCAGCAGAT ACCTGGCCTC 
CTTCCAGCTC ACGGGGAATC AGGAGATGCC CTCCGGAAGC CCCGCCTGCA GAAGCCTCTG 
ACGTGCTACC TGGATGATCT GTTCTTCACG CTGTATCCCT CGCTGGAGAA GTTCGAGGAG 
GAGCTTCTGG AACTCGTGAT CAGCGATTGC TTCCGGGAGG CGGGCAGCCC GCTGGACGTC 
GGCACCCTGG AGATCCTGGA GCGGCGCCTG TGCGTGGGCG TGCACAATGG GTTGGGCTTC 
GTGCAGCGGC CGCAGGTCCT CGTGCTGGTG CCCGAGCTGG AGGTGGCCTT GACACGCTCT 
GCCAGCTTCA GCCGGAAGGT CGGCTCCTCT TCCAAGGCCA GCTCTGGAAA CCAGGCGCTG 
GTCTTGAGAA GCCGTCTCCG ACTCCCTGAG ATGGTCCGTC ACCCCGCGTT CGCCATTGTC 
TTCCAGCTGG AGTACGTGTT TAACAGCCCC TCGGGGGCAG ACAGCAATGC AGCTTCACTC 
TCCTCCTTGT CCAGCCTGGC ATGTATGCAC ATGGTTCGCT GGGCTGTCTG GAACCCCTCG 
CTGGAAGCCA GCTCCGGGCA GGTGATGTTA CCTCTCCAGG GTGGCATCCA GTCCAACCCT 
TCGCACTGTC TGGTCTATAA GGTACCCCCA GCCAGCATAA GCTCCGAAGA GGTGAAGCAG 
GTGGAGTCAG GGACGGTCCA GTTCCAGTAC TCGCTGAGCC CTGAAGAACA CCCAGGCACA 
CCCACAGCGA ATGCAAGCAG CCCCAAGGCA GAGCGGCGGT CTTTTAGGAA GCCACCCGTG 
TCCCCTTCAA GTCCACCAGC ATCGCCGTCC CAGGGGCTCA CCGCTGCCCA GGACTCACCC 
ATGGGACCAG GGCTGTCACT TTCTCAGCTG GCGGCTTCCC CACCATCCCC AGCTCCCCGC 
CGCCGCTCGG CCAAACCTTC GTCACAGCCA CCTGGGAGCC CTGTGCCCTC CCTGGTCCCA 
GGGGAGTTCC CCTTGGAGGG CAGGATCTCT CACCTGGAGG CTGACCTGAG CCAGACAGCC 
TTAGCCCTGG GAACGTCCGC TGCTGACCAC TTGCAGGAAC TGCCTTTCAC ACCCGTGCAC 
GCCCCGATCA TTGCAGGAAC CCAGACCAGG AGCTCTGGAG GGAAGCTGTC ACGGGCATCC 
CTGGCGCTGC TGCAGTCCGC TGGCTTTCCC GAGATTCTGG ACGCCAACAA GCAACCAGCT 
GAGGCTGTGA GTCCTACAGA CCCCGTGAAG TTCAGCCCTC AGAAGGAAGA ATCAGACTGT 
CTACAAGGCA ATGAGATAGT GTTGCAATTT CTTGCCTTTA GCAGGGTGCC CCAGGATGAG 
CAGGCAGCGC CATGGCCACA GACTGTGTAC TTCACCTTCC AATTCTACCG CTTCCCACCC 
GAGACCACGC CACGGCTGCA GCTCGTGGAG CTGGCCGATG CTGGGAAGAC CAGCTCTGCC 
TCCCTGTCGC ACATCCTCGC CCAGATCCAG AAGGATGGCT CCTTTGATGC GGGGTCTCCG 
GGCTTTCAGC GGAAGTACCT GGTGGACCCC GGGTTCCTGA GACCCGGGGA GCAGCGCTGG 
TTCGCCCGCT ACCTGGCCAT GCAGACCCTG CAGATCGACG TCTGGGATGG GGACTCCCTG 
CTTCTCATTG GATCTGCTGC CGTTCAGCTG AAGCACCTTC TCCGCCAGGG GCGGCCGGCC 
GTGCAGGTCT CGCATGAGCT GGAGGTTGTG GCGACTGAAT ACGAGCAGGA CGCCATGGTG 
GTGAGTGGAG ACATGACCAA GTTTGGCTGC GTCAGACCCA TCGACGTCCA CACGGTGGTG 
AAGGGCCAGC TGCACTTGAC CTTGGCCAAC GTGGGTCACT TGTGTGAACA GAGAGTAAGG 
AGTCCCAGTT CCCTGCCACC TTCCAGGTCG AGGGTCATCT CAAATGATGG AGTTGGCAAC 
TTCATGGGAG GCAGCCTCCT GACACCCGGG GGCCCCAGAT CAGTGAAAAA TGTGGTCCAA 
GCTCAGAAGC TGGCTGATGT GGACAGCGAA CTGGCCGCCC TGCTCTTCAC ACAACCGAGA 
CCAGGGGCCC GGGGGCCCCA GGGCATCAAT CAAGAGGGGG ATGCCGTCCG CAGGCGCAAG 
CTGGAGCGCA TGAGGGCCGT GCGGCTGCGG GAATGTGGAT TGGAGCCGGG CCGCCGCGGG 
CTGGGCGCGC TGGCCCAGCA GAGCCTCCAT GCCCAGCACG CCCGAGACCT GCAGGTCATT 
GCCGCCTACC GTGAGCGCAC AAAGGCGGAG AGCATCGCCA GCGCACTGAG CACGGCCATC 
ACCACGCAGC ACACCATCCA CGCCACCCTA GGCGCTGCCG AGTTCTTCGA GTTCGCCCTG 
AGGAACCCCC ACAACGCGCA GCACATGGTG ACCATCGAGA TAGACAACCC CGAGCTCAGT 
CTCATCCTGG ATGGTCAGGA GTGGCGGTAC TTCAAGGACA TGGCTGACTT GCACACACCC 
CTGGAGGAGG ACATGTTCCA CCTGCGGGGC AGCCTGGCCC CCCAACTCTT CCTGCGACCT 
CGGGAGACTG CCCACATCCC CTTCAAGTTC CAGACTTTCT CTGTGGGCCC CTGGGCCTCG 
GCACAGGCCT CTGCTGAGCT GAGATCAGAG AAGGACATGG ATGCCGGATC ACCCTATAAG 
TCCAGCATGA TGCCCACCAA ACACGCCAAG GTCCTGTTCC GCGCGGGCAG CGGCAAGCCC 
CTGGCCGTGC TCTGCCTGAC GGTGGAGCTG CAGCCTCACG TGGTGGACCA GGTCTTCCGC 
TTCCACCACC CGGAGCTCAC CTTCCTGAAG AAAGCCATCC GCCTGCCACC CTGGCACACG 
CTTCCAGGTG CCCCTGTAGG GACTTCCAGT GAGGACCCCC CTTTCCATGT TCGATGTAGT 
GACCCGAACA TCATTTGTGA GACACAGAGT GTGGGCCCCG GGGAGCCCCG TGACGTGTTC 
CTGAAGGTGG CCAGTGGCCC TAGCCCAGAG GTCAAGGACT TCTTTGTCAC CATCTACGTA 
GACCGCTGGT TAGCAACACC TGTCCAGACC TGGCAGGTCC ACCTGCACTC GCTGCAGCGA 
GTGGACGTCT CCTGTATCAC TGGCCAGAGG ACCCGCCTGG CTCTCGTCCT CCGGGGGACG 
CAGACAGTTA GGAGAGTGCA GGCTTTCAGC TCACACCCCC AGGAGCTGAC GACGGACCCC 
AGAGGTGTGT TCGTGCTGCC TCCTCACGGG GTGCAGGACT TGCATGTGAG CGTCAGGCCC 
TGCAGGCCGG GCAGCCGCTT CGCGCACCTC CACCTGGTTG ACGTGGACGC CCACCAGCTG 
GTGGCCTCGT GGCTCCTGTG TCTCTCCTGC CGGCCGCCTC TCATCTCCAA GGCCTTCGAG 
ATAAAGCTGC CCGCGGGGAG CGGGAGGGGC GCCAATAAGC GCATCACCTA CACCAATCCC 
TACCCCTCGC GGAGGGCCTA CCGCCTGCAC AGCGACCACC CCCACCTGCT GCAGTTCAAG 
GAGGACGCCT TCCAGGTCGG GGGAGGTGAG ACCTATACCA TCGGCCTGCG GTTTGTGTCC 
CGTCAGAGAG CCGGGGAGGA AGAGATCCTG ATTTACATCA ACGACCACGA AGACAAAAAC 
GAGGAGACCT TTTGCGTGAA GGTCATCTAC CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832541.1
CDS	379..4674
Translation	MHDWHRIFDQNLLVPPHPQRARQPLKESTAFQCVLKWLDGPLIKQGVLELLSEVGCHLRVSLFDVTYRHFFGRTWRSSPRPAEPLTRQPARIVFNEPLYFHTSLNHPHIVAVVEVVVEGRRRDGTLQTLSCGFGILRIFSHKPESPTSTSQDKRLRLYHGSPRALLHPLLQDPIEQNKHMTLIENCSLQYTLRPHPLLEPVFHLLPENLLVSGLQQIPGLLPAHGESGDALRKPRLQKPLTCYLDDLFFTLYPSLEKFEEELLELVISDCFREAGSPLDVGTLEILERRLCVGVHNGLGFVQRPQVLVLVPELEVALTRSASFSRKVGSSSKASSGNQALVLRSRLRLPEMVRHPAFAIVFQLEYVFNSPSGADSNAASLSSLSSLACMHMVRWAVWNPSLEASSGQVMLPLQGGIQSNPSHCLVYKVPPASISSEEVKQVESGTVQFQYSLSPEEHPGTPTANASSPKAERRSFRKPPVSPSSPPASPSQGLTAAQDSPMGPGLSLSQLAASPPSPAPRRRSAKPSSQPPGSPVPSLVPGEFPLEGRISHLEADLSQTALALGTSAADHLQELPFTPVHAPIIAGTQTRSSGGKLSRASLALLQSAGFPEILDANKQPAEAVSPTDPVKFSPQKEESDCLQGNEIVLQFLAFSRVPQDEQAAPWPQTVYFTFQFYRFPPETTPRLQLVELADAGKTSSASLSHILAQIQKDGSFDAGSPGFQRKYLVDPGFLRPGEQRWFARYLAMQTLQIDVWDGDSLLLIGSAAVQLKHLLRQGRPAVQVSHELEVVATEYEQDAMVVSGDMTKFGCVRPIDVHTVVKGQLHLTLANVGHLCEQRVRSPSSLPPSRSRVISNDGVGNFMGGSLLTPGGPRSVKNVVQAQKLADVDSELAALLFTQPRPGARGPQGINQEGDAVRRRKLERMRAVRLRECGLEPGRRGLGALAQQSLHAQHARDLQVIAAYRERTKAESIASALSTAITTQHTIHATLGAAEFFEFALRNPHNAQHMVTIEIDNPELSLILDGQEWRYFKDMADLHTPLEEDMFHLRGSLAPQLFLRPRETAHIPFKFQTFSVGPWASAQASAELRSEKDMDAGSPYKSSMMPTKHAKVLFRAGSGKPLAVLCLTVELQPHVVDQVFRFHHPELTFLKKAIRLPPWHTLPGAPVGTSSEDPPFHVRCSDPNIICETQSVGPGEPRDVFLKVASGPSPEVKDFFVTIYVDRWLATPVQTWQVHLHSLQRVDVSCITGQRTRLALVLRGTQTVRRVQAFSSHPQELTTDPRGVFVLPPHGVQDLHVSVRPCRPGSRFAHLHLVDVDAHQLVASWLLCLSCRPPLISKAFEIKLPAGSGRGANKRITYTNPYPSRRAYRLHSDHPHLLQFKEDAFQVGGGETYTIGLRFVSRQRAGEEEILIYINDHEDKNEETFCVKVIYQ

Target ORF information:

RefSeq Version	XM_024976773.1
Organism	Bos taurus(cattle)
Definition	Bos taurus nephrocystin 4 (NPHP4), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976773.1

ORF Insert Sequence:

ATGCACGACT GGCACAGGAT CTTTGACCAA AACCTGCTGG TCCCTCCGCA CCCACAGAGA 
GCACGCCAGC CTCTGAAGGA ATCGACGGCG TTTCAGTGTG TCCTGAAGTG GCTTGATGGC 
CCGCTAATTA AGCAGGGGGT GCTGGAGCTG CTATCGGAGG TGGGCTGCCA CCTGCGCGTG 
TCTCTCTTCG ATGTCACCTA TCGGCACTTC TTCGGGAGGA CGTGGAGGAG CTCGCCGAGG 
CCTGCAGAGC CGCTCACCAG GCAGCCGGCC AGGATCGTCT TCAATGAGCC CTTGTATTTT 
CACACGTCCT TGAACCACCC TCATATTGTG GCCGTGGTGG AGGTGGTCGT TGAAGGCAGA 
AGACGAGACG GGACCCTCCA GACTCTGTCC TGTGGGTTTG GAATTCTTCG GATCTTCAGC 
CACAAGCCGG AATCTCCAAC TTCCACTTCC CAGGACAAAC GGCTGAGACT GTATCATGGG 
AGCCCGCGAG CCCTCCTCCA CCCGCTCCTT CAGGACCCCA TAGAACAGAA CAAGCACATG 
ACCCTCATTG AGAACTGCAG CCTGCAGTAC ACGCTGAGGC CACACCCGCT CCTGGAGCCC 
GTGTTCCACC TTCTTCCCGA GAACTTGCTG GTGTCCGGTC TGCAGCAGAT ACCTGGCCTC 
CTTCCAGCTC ACGGGGAATC AGGAGATGCC CTCCGGAAGC CCCGCCTGCA GAAGCCTCTG 
ACGTGCTACC TGGATGATCT GTTCTTCACG CTGTATCCCT CGCTGGAGAA GTTCGAGGAG 
GAGCTTCTGG AACTCGTGAT CAGCGATTGC TTCCGGGAGG CGGGCAGCCC GCTGGACGTC 
GGCACCCTGG AGATCCTGGA GCGGCGCCTG TGCGTGGGCG TGCACAATGG GTTGGGCTTC 
GTGCAGCGGC CGCAGGTCCT CGTGCTGGTG CCCGAGCTGG AGGTGGCCTT GACACGCTCT 
GCCAGCTTCA GCCGGAAGGT CGGCTCCTCT TCCAAGGCCA GCTCTGGAAA CCAGGCGCTG 
GTCTTGAGAA GCCGTCTCCG ACTCCCTGAG ATGGTCCGTC ACCCCGCGTT CGCCATTGTC 
TTCCAGCTGG AGTACGTGTT TAACAGCCCC TCGGGGGCAG ACAGCAATGC AGCTTCACTC 
TCCTCCTTGT CCAGCCTGGC ATGTATGCAC ATGGTTCGCT GGGCTGTCTG GAACCCCTCG 
CTGGAAGCCA GCTCCGGGCA GGTGATGTTA CCTCTCCAGG GTGGCATCCA GTCCAACCCT 
TCGCACTGTC TGGTCTATAA GGTACCCCCA GCCAGCATAA GCTCCGAAGA GGTGAAGCAG 
GTGGAGTCAG GGACGGTCCA GTTCCAGTAC TCGCTGAGCC CTGAAGAACA CCCAGGCACA 
CCCACAGCGA ATGCAAGCAG CCCCAAGGCA GAGCGGCGGT CTTTTAGGAA GCCACCCGTG 
TCCCCTTCAA GTCCACCAGC ATCGCCGTCC CAGGGGCTCA CCGCTGCCCA GGACTCACCC 
ATGGGACCAG GGCTGTCACT TTCTCAGCTG GCGGCTTCCC CACCATCCCC AGCTCCCCGC 
CGCCGCTCGG CCAAACCTTC GTCACAGCCA CCTGGGAGCC CTGTGCCCTC CCTGGTCCCA 
GGGGAGTTCC CCTTGGAGGG CAGGATCTCT CACCTGGAGG CTGACCTGAG CCAGACAGCC 
TTAGCCCTGG GAACGTCCGC TGCTGACCAC TTGCAGGAAC TGCCTTTCAC ACCCGTGCAC 
GCCCCGATCA TTGCAGGAAC CCAGACCAGG AGCTCTGGAG GGAAGCTGTC ACGGGCATCC 
CTGGCGCTGC TGCAGTCCGC TGGCTTTCCC GAGATTCTGG ACGCCAACAA GCAACCAGCT 
GAGGCTGTGA GTCCTACAGA CCCCGTGAAG TTCAGCCCTC AGAAGGAAGA ATCAGACTGT 
CTACAAGGCA ATGAGATAGT GTTGCAATTT CTTGCCTTTA GCAGGGTGCC CCAGGATGAG 
CAGGCAGCGC CATGGCCACA GACTGTGTAC TTCACCTTCC AATTCTACCG CTTCCCACCC 
GAGACCACGC CACGGCTGCA GCTCGTGGAG CTGGCCGATG CTGGGAAGAC CAGCTCTGCC 
TCCCTGTCGC ACATCCTCGC CCAGATCCAG AAGGATGGCT CCTTTGATGC GGGGTCTCCG 
GGCTTTCAGC GGAAGTACCT GGTGGACCCC GGGTTCCTGA GACCCGGGGA GCAGCGCTGG 
TTCGCCCGCT ACCTGGCCAT GCAGACCCTG CAGATCGACG TCTGGGATGG GGACTCCCTG 
CTTCTCATTG GATCTGCTGC CGTTCAGCTG AAGCACCTTC TCCGCCAGGG GCGGCCGGCC 
GTGCAGGTCT CGCATGAGCT GGAGGTTGTG GCGACTGAAT ACGAGCAGGA CGCCATGGTG 
GTGAGTGGAG ACATGACCAA GTTTGGCTGC GTCAGACCCA TCGACGTCCA CACGGTGGTG 
AAGGGCCAGC TGCACTTGAC CTTGGCCAAC GTGGGTCACT TGTGTGAACA GAGAGTAAGG 
AGTCCCAGTT CCCTGCCACC TTCCAGGTCG AGGGTCATCT CAAATGATGG AGTTGGCAAC 
TTCATGGGAG GCAGCCTCCT GACACCCGGG GGCCCCAGAT CAGTGAAAAA TGTGGTCCAA 
GCTCAGAAGC TGGCTGATGT GGACAGCGAA CTGGCCGCCC TGCTCTTCAC ACAACCGAGA 
CCAGGGGCCC GGGGGCCCCA GGGCATCAAT CAAGAGGGGG ATGCCGTCCG CAGGCGCAAG 
CTGGAGCGCA TGAGGGCCGT GCGGCTGCGG GAATGTGGAT TGGAGCCGGG CCGCCGCGGG 
CTGGGCGCGC TGGCCCAGCA GAGCCTCCAT GCCCAGCACG CCCGAGACCT GCAGGTCATT 
GCCGCCTACC GTGAGCGCAC AAAGGCGGAG AGCATCGCCA GCGCACTGAG CACGGCCATC 
ACCACGCAGC ACACCATCCA CGCCACCCTA GGCGCTGCCG AGTTCTTCGA GTTCGCCCTG 
AGGAACCCCC ACAACGCGCA GCACATGGTG ACCATCGAGA TAGACAACCC CGAGCTCAGT 
CTCATCCTGG ATGGTCAGGA GTGGCGGTAC TTCAAGGACA TGGCTGACTT GCACACACCC 
CTGGAGGAGG ACATGTTCCA CCTGCGGGGC AGCCTGGCCC CCCAACTCTT CCTGCGACCT 
CGGGAGACTG CCCACATCCC CTTCAAGTTC CAGACTTTCT CTGTGGGCCC CTGGGCCTCG 
GCACAGGCCT CTGCTGAGCT GAGATCAGAG AAGGACATGG ATGCCGGATC ACCCTATAAG 
TCCAGCATGA TGCCCACCAA ACACGCCAAG GTCCTGTTCC GCGCGGGCAG CGGCAAGCCC 
CTGGCCGTGC TCTGCCTGAC GGTGGAGCTG CAGCCTCACG TGGTGGACCA GGTCTTCCGC 
TTCCACCACC CGGAGCTCAC CTTCCTGAAG AAAGCCATCC GCCTGCCACC CTGGCACACG 
CTTCCAGGTG CCCCTGTAGG GACTTCCAGT GAGGACCCCC CTTTCCATGT TCGATGTAGT 
GACCCGAACA TCATTTGTGA GACACAGAGT GTGGGCCCCG GGGAGCCCCG TGACGTGTTC 
CTGAAGGTGG CCAGTGGCCC TAGCCCAGAG GTCAAGGACT TCTTTGTCAC CATCTACGTA 
GACCGCTGGT TAGCAACACC TGTCCAGACC TGGCAGGTCC ACCTGCACTC GCTGCAGCGA 
GTGGACGTCT CCTGTATCAC TGGCCAGAGG ACCCGCCTGG CTCTCGTCCT CCGGGGGACG 
CAGACAGTTA GGAGAGTGCA GGCTTTCAGC TCACACCCCC AGGAGCTGAC GACGGACCCC 
AGAGGTGTGT TCGTGCTGCC TCCTCACGGG GTGCAGGACT TGCATGTGAG CGTCAGGCCC 
TGCAGGCCGG GCAGCCGCTT CGCGCACCTC CACCTGGTTG ACGTGGACGC CCACCAGCTG 
GTGGCCTCGT GGCTCCTGTG TCTCTCCTGC CGGCCGCCTC TCATCTCCAA GGCCTTCGAG 
ATAAAGCTGC CCGCGGGGAG CGGGAGGGGC GCCAATAAGC GCATCACCTA CACCAATCCC 
TACCCCTCGC GGAGGGCCTA CCGCCTGCAC AGCGACCACC CCCACCTGCT GCAGTTCAAG 
GAGGACGCCT TCCAGGTCGG GGGAGGTGAG ACCTATACCA TCGGCCTGCG GTTTGTGTCC 
CGTCAGAGAG CCGGGGAGGA AGAGATCCTG ATTTACATCA ACGACCACGA AGACAAAAAC 
GAGGAGACCT TTTGCGTGAA GGTCATCTAC CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPHP4 ( XM_005217172.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005217229.1 Bos taurus nephrocystin 4 (NPHP4), transcript variant X6, mRNA.

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CloneID	OBa27367
Clone ID Related Accession (Same CDS sequence)	XM_015475180.1 , XM_015466842.1 , XM_015466845.1 , XM_024976773.1 , XM_005217172.4 , XM_015475179.1 , XM_015466844.1 , XM_015466846.1 , XM_005217172.3 , XM_010813390.2 , XM_024976775.1 , XM_024976776.1 , XM_005217171.3 , XM_024976772.1 , XM_010813390.3 , XM_015466843.1 , XM_005217171.4 , XM_015475180.2 , XM_024976774.1
Accession Version	XM_005217172.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4296bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	nephrocystin-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005217172.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACGACT GGCACAGGAT CTTTGACCAA AACCTGCTGG TCCCTCCGCA CCCACAGAGA 
GCACGCCAGC CTCTGAAGGA ATCGACGGCG TTTCAGTGTG TCCTGAAGTG GCTTGATGGC 
CCGCTAATTA AGCAGGGGGT GCTGGAGCTG CTATCGGAGG TGGGCTGCCA CCTGCGCGTG 
TCTCTCTTCG ATGTCACCTA TCGGCACTTC TTCGGGAGGA CGTGGAGGAG CTCGCCGAGG 
CCTGCAGAGC CGCTCACCAG GCAGCCGGCC AGGATCGTCT TCAATGAGCC CTTGTATTTT 
CACACGTCCT TGAACCACCC TCATATTGTG GCCGTGGTGG AGGTGGTCGT TGAAGGCAGA 
AGACGAGACG GGACCCTCCA GACTCTGTCC TGTGGGTTTG GAATTCTTCG GATCTTCAGC 
CACAAGCCGG AATCTCCAAC TTCCACTTCC CAGGACAAAC GGCTGAGACT GTATCATGGG 
AGCCCGCGAG CCCTCCTCCA CCCGCTCCTT CAGGACCCCA TAGAACAGAA CAAGCACATG 
ACCCTCATTG AGAACTGCAG CCTGCAGTAC ACGCTGAGGC CACACCCGCT CCTGGAGCCC 
GTGTTCCACC TTCTTCCCGA GAACTTGCTG GTGTCCGGTC TGCAGCAGAT ACCTGGCCTC 
CTTCCAGCTC ACGGGGAATC AGGAGATGCC CTCCGGAAGC CCCGCCTGCA GAAGCCTCTG 
ACGTGCTACC TGGATGATCT GTTCTTCACG CTGTATCCCT CGCTGGAGAA GTTCGAGGAG 
GAGCTTCTGG AACTCGTGAT CAGCGATTGC TTCCGGGAGG CGGGCAGCCC GCTGGACGTC 
GGCACCCTGG AGATCCTGGA GCGGCGCCTG TGCGTGGGCG TGCACAATGG GTTGGGCTTC 
GTGCAGCGGC CGCAGGTCCT CGTGCTGGTG CCCGAGCTGG AGGTGGCCTT GACACGCTCT 
GCCAGCTTCA GCCGGAAGGT CGGCTCCTCT TCCAAGGCCA GCTCTGGAAA CCAGGCGCTG 
GTCTTGAGAA GCCGTCTCCG ACTCCCTGAG ATGGTCCGTC ACCCCGCGTT CGCCATTGTC 
TTCCAGCTGG AGTACGTGTT TAACAGCCCC TCGGGGGCAG ACAGCAATGC AGCTTCACTC 
TCCTCCTTGT CCAGCCTGGC ATGTATGCAC ATGGTTCGCT GGGCTGTCTG GAACCCCTCG 
CTGGAAGCCA GCTCCGGGCA GGTGATGTTA CCTCTCCAGG GTGGCATCCA GTCCAACCCT 
TCGCACTGTC TGGTCTATAA GGTACCCCCA GCCAGCATAA GCTCCGAAGA GGTGAAGCAG 
GTGGAGTCAG GGACGGTCCA GTTCCAGTAC TCGCTGAGCC CTGAAGAACA CCCAGGCACA 
CCCACAGCGA ATGCAAGCAG CCCCAAGGCA GAGCGGCGGT CTTTTAGGAA GCCACCCGTG 
TCCCCTTCAA GTCCACCAGC ATCGCCGTCC CAGGGGCTCA CCGCTGCCCA GGACTCACCC 
ATGGGACCAG GGCTGTCACT TTCTCAGCTG GCGGCTTCCC CACCATCCCC AGCTCCCCGC 
CGCCGCTCGG CCAAACCTTC GTCACAGCCA CCTGGGAGCC CTGTGCCCTC CCTGGTCCCA 
GGGGAGTTCC CCTTGGAGGG CAGGATCTCT CACCTGGAGG CTGACCTGAG CCAGACAGCC 
TTAGCCCTGG GAACGTCCGC TGCTGACCAC TTGCAGGAAC TGCCTTTCAC ACCCGTGCAC 
GCCCCGATCA TTGCAGGAAC CCAGACCAGG AGCTCTGGAG GGAAGCTGTC ACGGGCATCC 
CTGGCGCTGC TGCAGTCCGC TGGCTTTCCC GAGATTCTGG ACGCCAACAA GCAACCAGCT 
GAGGCTGTGA GTCCTACAGA CCCCGTGAAG TTCAGCCCTC AGAAGGAAGA ATCAGACTGT 
CTACAAGGCA ATGAGATAGT GTTGCAATTT CTTGCCTTTA GCAGGGTGCC CCAGGATGAG 
CAGGCAGCGC CATGGCCACA GACTGTGTAC TTCACCTTCC AATTCTACCG CTTCCCACCC 
GAGACCACGC CACGGCTGCA GCTCGTGGAG CTGGCCGATG CTGGGAAGAC CAGCTCTGCC 
TCCCTGTCGC ACATCCTCGC CCAGATCCAG AAGGATGGCT CCTTTGATGC GGGGTCTCCG 
GGCTTTCAGC GGAAGTACCT GGTGGACCCC GGGTTCCTGA GACCCGGGGA GCAGCGCTGG 
TTCGCCCGCT ACCTGGCCAT GCAGACCCTG CAGATCGACG TCTGGGATGG GGACTCCCTG 
CTTCTCATTG GATCTGCTGC CGTTCAGCTG AAGCACCTTC TCCGCCAGGG GCGGCCGGCC 
GTGCAGGTCT CGCATGAGCT GGAGGTTGTG GCGACTGAAT ACGAGCAGGA CGCCATGGTG 
GTGAGTGGAG ACATGACCAA GTTTGGCTGC GTCAGACCCA TCGACGTCCA CACGGTGGTG 
AAGGGCCAGC TGCACTTGAC CTTGGCCAAC GTGGGTCACT TGTGTGAACA GAGAGTAAGG 
AGTCCCAGTT CCCTGCCACC TTCCAGGTCG AGGGTCATCT CAAATGATGG AGTTGGCAAC 
TTCATGGGAG GCAGCCTCCT GACACCCGGG GGCCCCAGAT CAGTGAAAAA TGTGGTCCAA 
GCTCAGAAGC TGGCTGATGT GGACAGCGAA CTGGCCGCCC TGCTCTTCAC ACAACCGAGA 
CCAGGGGCCC GGGGGCCCCA GGGCATCAAT CAAGAGGGGG ATGCCGTCCG CAGGCGCAAG 
CTGGAGCGCA TGAGGGCCGT GCGGCTGCGG GAATGTGGAT TGGAGCCGGG CCGCCGCGGG 
CTGGGCGCGC TGGCCCAGCA GAGCCTCCAT GCCCAGCACG CCCGAGACCT GCAGGTCATT 
GCCGCCTACC GTGAGCGCAC AAAGGCGGAG AGCATCGCCA GCGCACTGAG CACGGCCATC 
ACCACGCAGC ACACCATCCA CGCCACCCTA GGCGCTGCCG AGTTCTTCGA GTTCGCCCTG 
AGGAACCCCC ACAACGCGCA GCACATGGTG ACCATCGAGA TAGACAACCC CGAGCTCAGT 
CTCATCCTGG ATGGTCAGGA GTGGCGGTAC TTCAAGGACA TGGCTGACTT GCACACACCC 
CTGGAGGAGG ACATGTTCCA CCTGCGGGGC AGCCTGGCCC CCCAACTCTT CCTGCGACCT 
CGGGAGACTG CCCACATCCC CTTCAAGTTC CAGACTTTCT CTGTGGGCCC CTGGGCCTCG 
GCACAGGCCT CTGCTGAGCT GAGATCAGAG AAGGACATGG ATGCCGGATC ACCCTATAAG 
TCCAGCATGA TGCCCACCAA ACACGCCAAG GTCCTGTTCC GCGCGGGCAG CGGCAAGCCC 
CTGGCCGTGC TCTGCCTGAC GGTGGAGCTG CAGCCTCACG TGGTGGACCA GGTCTTCCGC 
TTCCACCACC CGGAGCTCAC CTTCCTGAAG AAAGCCATCC GCCTGCCACC CTGGCACACG 
CTTCCAGGTG CCCCTGTAGG GACTTCCAGT GAGGACCCCC CTTTCCATGT TCGATGTAGT 
GACCCGAACA TCATTTGTGA GACACAGAGT GTGGGCCCCG GGGAGCCCCG TGACGTGTTC 
CTGAAGGTGG CCAGTGGCCC TAGCCCAGAG GTCAAGGACT TCTTTGTCAC CATCTACGTA 
GACCGCTGGT TAGCAACACC TGTCCAGACC TGGCAGGTCC ACCTGCACTC GCTGCAGCGA 
GTGGACGTCT CCTGTATCAC TGGCCAGAGG ACCCGCCTGG CTCTCGTCCT CCGGGGGACG 
CAGACAGTTA GGAGAGTGCA GGCTTTCAGC TCACACCCCC AGGAGCTGAC GACGGACCCC 
AGAGGTGTGT TCGTGCTGCC TCCTCACGGG GTGCAGGACT TGCATGTGAG CGTCAGGCCC 
TGCAGGCCGG GCAGCCGCTT CGCGCACCTC CACCTGGTTG ACGTGGACGC CCACCAGCTG 
GTGGCCTCGT GGCTCCTGTG TCTCTCCTGC CGGCCGCCTC TCATCTCCAA GGCCTTCGAG 
ATAAAGCTGC CCGCGGGGAG CGGGAGGGGC GCCAATAAGC GCATCACCTA CACCAATCCC 
TACCCCTCGC GGAGGGCCTA CCGCCTGCAC AGCGACCACC CCCACCTGCT GCAGTTCAAG 
GAGGACGCCT TCCAGGTCGG GGGAGGTGAG ACCTATACCA TCGGCCTGCG GTTTGTGTCC 
CGTCAGAGAG CCGGGGAGGA AGAGATCCTG ATTTACATCA ACGACCACGA AGACAAAAAC 
GAGGAGACCT TTTGCGTGAA GGTCATCTAC CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005217229.1
CDS	1907..6202
Translation	MHDWHRIFDQNLLVPPHPQRARQPLKESTAFQCVLKWLDGPLIKQGVLELLSEVGCHLRVSLFDVTYRHFFGRTWRSSPRPAEPLTRQPARIVFNEPLYFHTSLNHPHIVAVVEVVVEGRRRDGTLQTLSCGFGILRIFSHKPESPTSTSQDKRLRLYHGSPRALLHPLLQDPIEQNKHMTLIENCSLQYTLRPHPLLEPVFHLLPENLLVSGLQQIPGLLPAHGESGDALRKPRLQKPLTCYLDDLFFTLYPSLEKFEEELLELVISDCFREAGSPLDVGTLEILERRLCVGVHNGLGFVQRPQVLVLVPELEVALTRSASFSRKVGSSSKASSGNQALVLRSRLRLPEMVRHPAFAIVFQLEYVFNSPSGADSNAASLSSLSSLACMHMVRWAVWNPSLEASSGQVMLPLQGGIQSNPSHCLVYKVPPASISSEEVKQVESGTVQFQYSLSPEEHPGTPTANASSPKAERRSFRKPPVSPSSPPASPSQGLTAAQDSPMGPGLSLSQLAASPPSPAPRRRSAKPSSQPPGSPVPSLVPGEFPLEGRISHLEADLSQTALALGTSAADHLQELPFTPVHAPIIAGTQTRSSGGKLSRASLALLQSAGFPEILDANKQPAEAVSPTDPVKFSPQKEESDCLQGNEIVLQFLAFSRVPQDEQAAPWPQTVYFTFQFYRFPPETTPRLQLVELADAGKTSSASLSHILAQIQKDGSFDAGSPGFQRKYLVDPGFLRPGEQRWFARYLAMQTLQIDVWDGDSLLLIGSAAVQLKHLLRQGRPAVQVSHELEVVATEYEQDAMVVSGDMTKFGCVRPIDVHTVVKGQLHLTLANVGHLCEQRVRSPSSLPPSRSRVISNDGVGNFMGGSLLTPGGPRSVKNVVQAQKLADVDSELAALLFTQPRPGARGPQGINQEGDAVRRRKLERMRAVRLRECGLEPGRRGLGALAQQSLHAQHARDLQVIAAYRERTKAESIASALSTAITTQHTIHATLGAAEFFEFALRNPHNAQHMVTIEIDNPELSLILDGQEWRYFKDMADLHTPLEEDMFHLRGSLAPQLFLRPRETAHIPFKFQTFSVGPWASAQASAELRSEKDMDAGSPYKSSMMPTKHAKVLFRAGSGKPLAVLCLTVELQPHVVDQVFRFHHPELTFLKKAIRLPPWHTLPGAPVGTSSEDPPFHVRCSDPNIICETQSVGPGEPRDVFLKVASGPSPEVKDFFVTIYVDRWLATPVQTWQVHLHSLQRVDVSCITGQRTRLALVLRGTQTVRRVQAFSSHPQELTTDPRGVFVLPPHGVQDLHVSVRPCRPGSRFAHLHLVDVDAHQLVASWLLCLSCRPPLISKAFEIKLPAGSGRGANKRITYTNPYPSRRAYRLHSDHPHLLQFKEDAFQVGGGETYTIGLRFVSRQRAGEEEILIYINDHEDKNEETFCVKVIYQ

Target ORF information:

RefSeq Version	XM_005217172.4
Organism	Bos taurus(cattle)
Definition	Bos taurus nephrocystin 4 (NPHP4), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005217172.4

ORF Insert Sequence:

ATGCACGACT GGCACAGGAT CTTTGACCAA AACCTGCTGG TCCCTCCGCA CCCACAGAGA 
GCACGCCAGC CTCTGAAGGA ATCGACGGCG TTTCAGTGTG TCCTGAAGTG GCTTGATGGC 
CCGCTAATTA AGCAGGGGGT GCTGGAGCTG CTATCGGAGG TGGGCTGCCA CCTGCGCGTG 
TCTCTCTTCG ATGTCACCTA TCGGCACTTC TTCGGGAGGA CGTGGAGGAG CTCGCCGAGG 
CCTGCAGAGC CGCTCACCAG GCAGCCGGCC AGGATCGTCT TCAATGAGCC CTTGTATTTT 
CACACGTCCT TGAACCACCC TCATATTGTG GCCGTGGTGG AGGTGGTCGT TGAAGGCAGA 
AGACGAGACG GGACCCTCCA GACTCTGTCC TGTGGGTTTG GAATTCTTCG GATCTTCAGC 
CACAAGCCGG AATCTCCAAC TTCCACTTCC CAGGACAAAC GGCTGAGACT GTATCATGGG 
AGCCCGCGAG CCCTCCTCCA CCCGCTCCTT CAGGACCCCA TAGAACAGAA CAAGCACATG 
ACCCTCATTG AGAACTGCAG CCTGCAGTAC ACGCTGAGGC CACACCCGCT CCTGGAGCCC 
GTGTTCCACC TTCTTCCCGA GAACTTGCTG GTGTCCGGTC TGCAGCAGAT ACCTGGCCTC 
CTTCCAGCTC ACGGGGAATC AGGAGATGCC CTCCGGAAGC CCCGCCTGCA GAAGCCTCTG 
ACGTGCTACC TGGATGATCT GTTCTTCACG CTGTATCCCT CGCTGGAGAA GTTCGAGGAG 
GAGCTTCTGG AACTCGTGAT CAGCGATTGC TTCCGGGAGG CGGGCAGCCC GCTGGACGTC 
GGCACCCTGG AGATCCTGGA GCGGCGCCTG TGCGTGGGCG TGCACAATGG GTTGGGCTTC 
GTGCAGCGGC CGCAGGTCCT CGTGCTGGTG CCCGAGCTGG AGGTGGCCTT GACACGCTCT 
GCCAGCTTCA GCCGGAAGGT CGGCTCCTCT TCCAAGGCCA GCTCTGGAAA CCAGGCGCTG 
GTCTTGAGAA GCCGTCTCCG ACTCCCTGAG ATGGTCCGTC ACCCCGCGTT CGCCATTGTC 
TTCCAGCTGG AGTACGTGTT TAACAGCCCC TCGGGGGCAG ACAGCAATGC AGCTTCACTC 
TCCTCCTTGT CCAGCCTGGC ATGTATGCAC ATGGTTCGCT GGGCTGTCTG GAACCCCTCG 
CTGGAAGCCA GCTCCGGGCA GGTGATGTTA CCTCTCCAGG GTGGCATCCA GTCCAACCCT 
TCGCACTGTC TGGTCTATAA GGTACCCCCA GCCAGCATAA GCTCCGAAGA GGTGAAGCAG 
GTGGAGTCAG GGACGGTCCA GTTCCAGTAC TCGCTGAGCC CTGAAGAACA CCCAGGCACA 
CCCACAGCGA ATGCAAGCAG CCCCAAGGCA GAGCGGCGGT CTTTTAGGAA GCCACCCGTG 
TCCCCTTCAA GTCCACCAGC ATCGCCGTCC CAGGGGCTCA CCGCTGCCCA GGACTCACCC 
ATGGGACCAG GGCTGTCACT TTCTCAGCTG GCGGCTTCCC CACCATCCCC AGCTCCCCGC 
CGCCGCTCGG CCAAACCTTC GTCACAGCCA CCTGGGAGCC CTGTGCCCTC CCTGGTCCCA 
GGGGAGTTCC CCTTGGAGGG CAGGATCTCT CACCTGGAGG CTGACCTGAG CCAGACAGCC 
TTAGCCCTGG GAACGTCCGC TGCTGACCAC TTGCAGGAAC TGCCTTTCAC ACCCGTGCAC 
GCCCCGATCA TTGCAGGAAC CCAGACCAGG AGCTCTGGAG GGAAGCTGTC ACGGGCATCC 
CTGGCGCTGC TGCAGTCCGC TGGCTTTCCC GAGATTCTGG ACGCCAACAA GCAACCAGCT 
GAGGCTGTGA GTCCTACAGA CCCCGTGAAG TTCAGCCCTC AGAAGGAAGA ATCAGACTGT 
CTACAAGGCA ATGAGATAGT GTTGCAATTT CTTGCCTTTA GCAGGGTGCC CCAGGATGAG 
CAGGCAGCGC CATGGCCACA GACTGTGTAC TTCACCTTCC AATTCTACCG CTTCCCACCC 
GAGACCACGC CACGGCTGCA GCTCGTGGAG CTGGCCGATG CTGGGAAGAC CAGCTCTGCC 
TCCCTGTCGC ACATCCTCGC CCAGATCCAG AAGGATGGCT CCTTTGATGC GGGGTCTCCG 
GGCTTTCAGC GGAAGTACCT GGTGGACCCC GGGTTCCTGA GACCCGGGGA GCAGCGCTGG 
TTCGCCCGCT ACCTGGCCAT GCAGACCCTG CAGATCGACG TCTGGGATGG GGACTCCCTG 
CTTCTCATTG GATCTGCTGC CGTTCAGCTG AAGCACCTTC TCCGCCAGGG GCGGCCGGCC 
GTGCAGGTCT CGCATGAGCT GGAGGTTGTG GCGACTGAAT ACGAGCAGGA CGCCATGGTG 
GTGAGTGGAG ACATGACCAA GTTTGGCTGC GTCAGACCCA TCGACGTCCA CACGGTGGTG 
AAGGGCCAGC TGCACTTGAC CTTGGCCAAC GTGGGTCACT TGTGTGAACA GAGAGTAAGG 
AGTCCCAGTT CCCTGCCACC TTCCAGGTCG AGGGTCATCT CAAATGATGG AGTTGGCAAC 
TTCATGGGAG GCAGCCTCCT GACACCCGGG GGCCCCAGAT CAGTGAAAAA TGTGGTCCAA 
GCTCAGAAGC TGGCTGATGT GGACAGCGAA CTGGCCGCCC TGCTCTTCAC ACAACCGAGA 
CCAGGGGCCC GGGGGCCCCA GGGCATCAAT CAAGAGGGGG ATGCCGTCCG CAGGCGCAAG 
CTGGAGCGCA TGAGGGCCGT GCGGCTGCGG GAATGTGGAT TGGAGCCGGG CCGCCGCGGG 
CTGGGCGCGC TGGCCCAGCA GAGCCTCCAT GCCCAGCACG CCCGAGACCT GCAGGTCATT 
GCCGCCTACC GTGAGCGCAC AAAGGCGGAG AGCATCGCCA GCGCACTGAG CACGGCCATC 
ACCACGCAGC ACACCATCCA CGCCACCCTA GGCGCTGCCG AGTTCTTCGA GTTCGCCCTG 
AGGAACCCCC ACAACGCGCA GCACATGGTG ACCATCGAGA TAGACAACCC CGAGCTCAGT 
CTCATCCTGG ATGGTCAGGA GTGGCGGTAC TTCAAGGACA TGGCTGACTT GCACACACCC 
CTGGAGGAGG ACATGTTCCA CCTGCGGGGC AGCCTGGCCC CCCAACTCTT CCTGCGACCT 
CGGGAGACTG CCCACATCCC CTTCAAGTTC CAGACTTTCT CTGTGGGCCC CTGGGCCTCG 
GCACAGGCCT CTGCTGAGCT GAGATCAGAG AAGGACATGG ATGCCGGATC ACCCTATAAG 
TCCAGCATGA TGCCCACCAA ACACGCCAAG GTCCTGTTCC GCGCGGGCAG CGGCAAGCCC 
CTGGCCGTGC TCTGCCTGAC GGTGGAGCTG CAGCCTCACG TGGTGGACCA GGTCTTCCGC 
TTCCACCACC CGGAGCTCAC CTTCCTGAAG AAAGCCATCC GCCTGCCACC CTGGCACACG 
CTTCCAGGTG CCCCTGTAGG GACTTCCAGT GAGGACCCCC CTTTCCATGT TCGATGTAGT 
GACCCGAACA TCATTTGTGA GACACAGAGT GTGGGCCCCG GGGAGCCCCG TGACGTGTTC 
CTGAAGGTGG CCAGTGGCCC TAGCCCAGAG GTCAAGGACT TCTTTGTCAC CATCTACGTA 
GACCGCTGGT TAGCAACACC TGTCCAGACC TGGCAGGTCC ACCTGCACTC GCTGCAGCGA 
GTGGACGTCT CCTGTATCAC TGGCCAGAGG ACCCGCCTGG CTCTCGTCCT CCGGGGGACG 
CAGACAGTTA GGAGAGTGCA GGCTTTCAGC TCACACCCCC AGGAGCTGAC GACGGACCCC 
AGAGGTGTGT TCGTGCTGCC TCCTCACGGG GTGCAGGACT TGCATGTGAG CGTCAGGCCC 
TGCAGGCCGG GCAGCCGCTT CGCGCACCTC CACCTGGTTG ACGTGGACGC CCACCAGCTG 
GTGGCCTCGT GGCTCCTGTG TCTCTCCTGC CGGCCGCCTC TCATCTCCAA GGCCTTCGAG 
ATAAAGCTGC CCGCGGGGAG CGGGAGGGGC GCCAATAAGC GCATCACCTA CACCAATCCC 
TACCCCTCGC GGAGGGCCTA CCGCCTGCAC AGCGACCACC CCCACCTGCT GCAGTTCAAG 
GAGGACGCCT TCCAGGTCGG GGGAGGTGAG ACCTATACCA TCGGCCTGCG GTTTGTGTCC 
CGTCAGAGAG CCGGGGAGGA AGAGATCCTG ATTTACATCA ACGACCACGA AGACAAAAAC 
GAGGAGACCT TTTGCGTGAA GGTCATCTAC CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPHP4 ( XM_015475179.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015330665.1 Bos taurus nephronophthisis 4 (NPHP4), transcript variant X3, mRNA.

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CloneID	OBa27367
Clone ID Related Accession (Same CDS sequence)	XM_015475180.1 , XM_015466842.1 , XM_015466845.1 , XM_024976773.1 , XM_005217172.4 , XM_015475179.1 , XM_015466844.1 , XM_015466846.1 , XM_005217172.3 , XM_010813390.2 , XM_024976775.1 , XM_024976776.1 , XM_005217171.3 , XM_024976772.1 , XM_010813390.3 , XM_015466843.1 , XM_005217171.4 , XM_015475180.2 , XM_024976774.1
Accession Version	XM_015475179.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4296bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	nephrocystin-4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104439.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACGACT GGCACAGGAT CTTTGACCAA AACCTGCTGG TCCCTCCGCA CCCACAGAGA 
GCACGCCAGC CTCTGAAGGA ATCGACGGCG TTTCAGTGTG TCCTGAAGTG GCTTGATGGC 
CCGCTAATTA AGCAGGGGGT GCTGGAGCTG CTATCGGAGG TGGGCTGCCA CCTGCGCGTG 
TCTCTCTTCG ATGTCACCTA TCGGCACTTC TTCGGGAGGA CGTGGAGGAG CTCGCCGAGG 
CCTGCAGAGC CGCTCACCAG GCAGCCGGCC AGGATCGTCT TCAATGAGCC CTTGTATTTT 
CACACGTCCT TGAACCACCC TCATATTGTG GCCGTGGTGG AGGTGGTCGT TGAAGGCAGA 
AGACGAGACG GGACCCTCCA GACTCTGTCC TGTGGGTTTG GAATTCTTCG GATCTTCAGC 
CACAAGCCGG AATCTCCAAC TTCCACTTCC CAGGACAAAC GGCTGAGACT GTATCATGGG 
AGCCCGCGAG CCCTCCTCCA CCCGCTCCTT CAGGACCCCA TAGAACAGAA CAAGCACATG 
ACCCTCATTG AGAACTGCAG CCTGCAGTAC ACGCTGAGGC CACACCCGCT CCTGGAGCCC 
GTGTTCCACC TTCTTCCCGA GAACTTGCTG GTGTCCGGTC TGCAGCAGAT ACCTGGCCTC 
CTTCCAGCTC ACGGGGAATC AGGAGATGCC CTCCGGAAGC CCCGCCTGCA GAAGCCTCTG 
ACGTGCTACC TGGATGATCT GTTCTTCACG CTGTATCCCT CGCTGGAGAA GTTCGAGGAG 
GAGCTTCTGG AACTCGTGAT CAGCGATTGC TTCCGGGAGG CGGGCAGCCC GCTGGACGTC 
GGCACCCTGG AGATCCTGGA GCGGCGCCTG TGCGTGGGCG TGCACAATGG GTTGGGCTTC 
GTGCAGCGGC CGCAGGTCCT CGTGCTGGTG CCCGAGCTGG AGGTGGCCTT GACACGCTCT 
GCCAGCTTCA GCCGGAAGGT CGGCTCCTCT TCCAAGGCCA GCTCTGGAAA CCAGGCGCTG 
GTCTTGAGAA GCCGTCTCCG ACTCCCTGAG ATGGTCCGTC ACCCCGCGTT CGCCATTGTC 
TTCCAGCTGG AGTACGTGTT TAACAGCCCC TCGGGGGCAG ACAGCAATGC AGCTTCACTC 
TCCTCCTTGT CCAGCCTGGC ATGTATGCAC ATGGTTCGCT GGGCTGTCTG GAACCCCTCG 
CTGGAAGCCA GCTCCGGGCA GGTGATGTTA CCTCTCCAGG GTGGCATCCA GTCCAACCCT 
TCGCACTGTC TGGTCTATAA GGTACCCCCA GCCAGCATAA GCTCCGAAGA GGTGAAGCAG 
GTGGAGTCAG GGACGGTCCA GTTCCAGTAC TCGCTGAGCC CTGAAGAACA CCCAGGCACA 
CCCACAGCGA ATGCAAGCAG CCCCAAGGCA GAGCGGCGGT CTTTTAGGAA GCCACCCGTG 
TCCCCTTCAA GTCCACCAGC ATCGCCGTCC CAGGGGCTCA CCGCTGCCCA GGACTCACCC 
ATGGGACCAG GGCTGTCACT TTCTCAGCTG GCGGCTTCCC CACCATCCCC AGCTCCCCGC 
CGCCGCTCGG CCAAACCTTC GTCACAGCCA CCTGGGAGCC CTGTGCCCTC CCTGGTCCCA 
GGGGAGTTCC CCTTGGAGGG CAGGATCTCT CACCTGGAGG CTGACCTGAG CCAGACAGCC 
TTAGCCCTGG GAACGTCCGC TGCTGACCAC TTGCAGGAAC TGCCTTTCAC ACCCGTGCAC 
GCCCCGATCA TTGCAGGAAC CCAGACCAGG AGCTCTGGAG GGAAGCTGTC ACGGGCATCC 
CTGGCGCTGC TGCAGTCCGC TGGCTTTCCC GAGATTCTGG ACGCCAACAA GCAACCAGCT 
GAGGCTGTGA GTCCTACAGA CCCCGTGAAG TTCAGCCCTC AGAAGGAAGA ATCAGACTGT 
CTACAAGGCA ATGAGATAGT GTTGCAATTT CTTGCCTTTA GCAGGGTGCC CCAGGATGAG 
CAGGCAGCGC CATGGCCACA GACTGTGTAC TTCACCTTCC AATTCTACCG CTTCCCACCC 
GAGACCACGC CACGGCTGCA GCTCGTGGAG CTGGCCGATG CTGGGAAGAC CAGCTCTGCC 
TCCCTGTCGC ACATCCTCGC CCAGATCCAG AAGGATGGCT CCTTTGATGC GGGGTCTCCG 
GGCTTTCAGC GGAAGTACCT GGTGGACCCC GGGTTCCTGA GACCCGGGGA GCAGCGCTGG 
TTCGCCCGCT ACCTGGCCAT GCAGACCCTG CAGATCGACG TCTGGGATGG GGACTCCCTG 
CTTCTCATTG GATCTGCTGC CGTTCAGCTG AAGCACCTTC TCCGCCAGGG GCGGCCGGCC 
GTGCAGGTCT CGCATGAGCT GGAGGTTGTG GCGACTGAAT ACGAGCAGGA CGCCATGGTG 
GTGAGTGGAG ACATGACCAA GTTTGGCTGC GTCAGACCCA TCGACGTCCA CACGGTGGTG 
AAGGGCCAGC TGCACTTGAC CTTGGCCAAC GTGGGTCACT TGTGTGAACA GAGAGTAAGG 
AGTCCCAGTT CCCTGCCACC TTCCAGGTCG AGGGTCATCT CAAATGATGG AGTTGGCAAC 
TTCATGGGAG GCAGCCTCCT GACACCCGGG GGCCCCAGAT CAGTGAAAAA TGTGGTCCAA 
GCTCAGAAGC TGGCTGATGT GGACAGCGAA CTGGCCGCCC TGCTCTTCAC ACAACCGAGA 
CCAGGGGCCC GGGGGCCCCA GGGCATCAAT CAAGAGGGGG ATGCCGTCCG CAGGCGCAAG 
CTGGAGCGCA TGAGGGCCGT GCGGCTGCGG GAATGTGGAT TGGAGCCGGG CCGCCGCGGG 
CTGGGCGCGC TGGCCCAGCA GAGCCTCCAT GCCCAGCACG CCCGAGACCT GCAGGTCATT 
GCCGCCTACC GTGAGCGCAC AAAGGCGGAG AGCATCGCCA GCGCACTGAG CACGGCCATC 
ACCACGCAGC ACACCATCCA CGCCACCCTA GGCGCTGCCG AGTTCTTCGA GTTCGCCCTG 
AGGAACCCCC ACAACGCGCA GCACATGGTG ACCATCGAGA TAGACAACCC CGAGCTCAGT 
CTCATCCTGG ATGGTCAGGA GTGGCGGTAC TTCAAGGACA TGGCTGACTT GCACACACCC 
CTGGAGGAGG ACATGTTCCA CCTGCGGGGC AGCCTGGCCC CCCAACTCTT CCTGCGACCT 
CGGGAGACTG CCCACATCCC CTTCAAGTTC CAGACTTTCT CTGTGGGCCC CTGGGCCTCG 
GCACAGGCCT CTGCTGAGCT GAGATCAGAG AAGGACATGG ATGCCGGATC ACCCTATAAG 
TCCAGCATGA TGCCCACCAA ACACGCCAAG GTCCTGTTCC GCGCGGGCAG CGGCAAGCCC 
CTGGCCGTGC TCTGCCTGAC GGTGGAGCTG CAGCCTCACG TGGTGGACCA GGTCTTCCGC 
TTCCACCACC CGGAGCTCAC CTTCCTGAAG AAAGCCATCC GCCTGCCACC CTGGCACACG 
CTTCCAGGTG CCCCTGTAGG GACTTCCAGT GAGGACCCCC CTTTCCATGT TCGATGTAGT 
GACCCGAACA TCATTTGTGA GACACAGAGT GTGGGCCCCG GGGAGCCCCG TGACGTGTTC 
CTGAAGGTGG CCAGTGGCCC TAGCCCAGAG GTCAAGGACT TCTTTGTCAC CATCTACGTA 
GACCGCTGGT TAGCAACACC TGTCCAGACC TGGCAGGTCC ACCTGCACTC GCTGCAGCGA 
GTGGACGTCT CCTGTATCAC TGGCCAGAGG ACCCGCCTGG CTCTCGTCCT CCGGGGGACG 
CAGACAGTTA GGAGAGTGCA GGCTTTCAGC TCACACCCCC AGGAGCTGAC GACGGACCCC 
AGAGGTGTGT TCGTGCTGCC TCCTCACGGG GTGCAGGACT TGCATGTGAG CGTCAGGCCC 
TGCAGGCCGG GCAGCCGCTT CGCGCACCTC CACCTGGTTG ACGTGGACGC CCACCAGCTG 
GTGGCCTCGT GGCTCCTGTG TCTCTCCTGC CGGCCGCCTC TCATCTCCAA GGCCTTCGAG 
ATAAAGCTGC CCGCGGGGAG CGGGAGGGGC GCCAATAAGC GCATCACCTA CACCAATCCC 
TACCCCTCGC GGAGGGCCTA CCGCCTGCAC AGCGACCACC CCCACCTGCT GCAGTTCAAG 
GAGGACGCCT TCCAGGTCGG GGGAGGTGAG ACCTATACCA TCGGCCTGCG GTTTGTGTCC 
CGTCAGAGAG CCGGGGAGGA AGAGATCCTG ATTTACATCA ACGACCACGA AGACAAAAAC 
GAGGAGACCT TTTGCGTGAA GGTCATCTAC CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015330665.1
CDS	586..4881
Translation	MHDWHRIFDQNLLVPPHPQRARQPLKESTAFQCVLKWLDGPLIKQGVLELLSEVGCHLRVSLFDVTYRHFFGRTWRSSPRPAEPLTRQPARIVFNEPLYFHTSLNHPHIVAVVEVVVEGRRRDGTLQTLSCGFGILRIFSHKPESPTSTSQDKRLRLYHGSPRALLHPLLQDPIEQNKHMTLIENCSLQYTLRPHPLLEPVFHLLPENLLVSGLQQIPGLLPAHGESGDALRKPRLQKPLTCYLDDLFFTLYPSLEKFEEELLELVISDCFREAGSPLDVGTLEILERRLCVGVHNGLGFVQRPQVLVLVPELEVALTRSASFSRKVGSSSKASSGNQALVLRSRLRLPEMVRHPAFAIVFQLEYVFNSPSGADSNAASLSSLSSLACMHMVRWAVWNPSLEASSGQVMLPLQGGIQSNPSHCLVYKVPPASISSEEVKQVESGTVQFQYSLSPEEHPGTPTANASSPKAERRSFRKPPVSPSSPPASPSQGLTAAQDSPMGPGLSLSQLAASPPSPAPRRRSAKPSSQPPGSPVPSLVPGEFPLEGRISHLEADLSQTALALGTSAADHLQELPFTPVHAPIIAGTQTRSSGGKLSRASLALLQSAGFPEILDANKQPAEAVSPTDPVKFSPQKEESDCLQGNEIVLQFLAFSRVPQDEQAAPWPQTVYFTFQFYRFPPETTPRLQLVELADAGKTSSASLSHILAQIQKDGSFDAGSPGFQRKYLVDPGFLRPGEQRWFARYLAMQTLQIDVWDGDSLLLIGSAAVQLKHLLRQGRPAVQVSHELEVVATEYEQDAMVVSGDMTKFGCVRPIDVHTVVKGQLHLTLANVGHLCEQRVRSPSSLPPSRSRVISNDGVGNFMGGSLLTPGGPRSVKNVVQAQKLADVDSELAALLFTQPRPGARGPQGINQEGDAVRRRKLERMRAVRLRECGLEPGRRGLGALAQQSLHAQHARDLQVIAAYRERTKAESIASALSTAITTQHTIHATLGAAEFFEFALRNPHNAQHMVTIEIDNPELSLILDGQEWRYFKDMADLHTPLEEDMFHLRGSLAPQLFLRPRETAHIPFKFQTFSVGPWASAQASAELRSEKDMDAGSPYKSSMMPTKHAKVLFRAGSGKPLAVLCLTVELQPHVVDQVFRFHHPELTFLKKAIRLPPWHTLPGAPVGTSSEDPPFHVRCSDPNIICETQSVGPGEPRDVFLKVASGPSPEVKDFFVTIYVDRWLATPVQTWQVHLHSLQRVDVSCITGQRTRLALVLRGTQTVRRVQAFSSHPQELTTDPRGVFVLPPHGVQDLHVSVRPCRPGSRFAHLHLVDVDAHQLVASWLLCLSCRPPLISKAFEIKLPAGSGRGANKRITYTNPYPSRRAYRLHSDHPHLLQFKEDAFQVGGGETYTIGLRFVSRQRAGEEEILIYINDHEDKNEETFCVKVIYQ

Target ORF information:

RefSeq Version	XM_015475179.1
Organism	Bos taurus(cattle)
Definition	Bos taurus nephronophthisis 4 (NPHP4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015475179.1

ORF Insert Sequence:

ATGCACGACT GGCACAGGAT CTTTGACCAA AACCTGCTGG TCCCTCCGCA CCCACAGAGA 
GCACGCCAGC CTCTGAAGGA ATCGACGGCG TTTCAGTGTG TCCTGAAGTG GCTTGATGGC 
CCGCTAATTA AGCAGGGGGT GCTGGAGCTG CTATCGGAGG TGGGCTGCCA CCTGCGCGTG 
TCTCTCTTCG ATGTCACCTA TCGGCACTTC TTCGGGAGGA CGTGGAGGAG CTCGCCGAGG 
CCTGCAGAGC CGCTCACCAG GCAGCCGGCC AGGATCGTCT TCAATGAGCC CTTGTATTTT 
CACACGTCCT TGAACCACCC TCATATTGTG GCCGTGGTGG AGGTGGTCGT TGAAGGCAGA 
AGACGAGACG GGACCCTCCA GACTCTGTCC TGTGGGTTTG GAATTCTTCG GATCTTCAGC 
CACAAGCCGG AATCTCCAAC TTCCACTTCC CAGGACAAAC GGCTGAGACT GTATCATGGG 
AGCCCGCGAG CCCTCCTCCA CCCGCTCCTT CAGGACCCCA TAGAACAGAA CAAGCACATG 
ACCCTCATTG AGAACTGCAG CCTGCAGTAC ACGCTGAGGC CACACCCGCT CCTGGAGCCC 
GTGTTCCACC TTCTTCCCGA GAACTTGCTG GTGTCCGGTC TGCAGCAGAT ACCTGGCCTC 
CTTCCAGCTC ACGGGGAATC AGGAGATGCC CTCCGGAAGC CCCGCCTGCA GAAGCCTCTG 
ACGTGCTACC TGGATGATCT GTTCTTCACG CTGTATCCCT CGCTGGAGAA GTTCGAGGAG 
GAGCTTCTGG AACTCGTGAT CAGCGATTGC TTCCGGGAGG CGGGCAGCCC GCTGGACGTC 
GGCACCCTGG AGATCCTGGA GCGGCGCCTG TGCGTGGGCG TGCACAATGG GTTGGGCTTC 
GTGCAGCGGC CGCAGGTCCT CGTGCTGGTG CCCGAGCTGG AGGTGGCCTT GACACGCTCT 
GCCAGCTTCA GCCGGAAGGT CGGCTCCTCT TCCAAGGCCA GCTCTGGAAA CCAGGCGCTG 
GTCTTGAGAA GCCGTCTCCG ACTCCCTGAG ATGGTCCGTC ACCCCGCGTT CGCCATTGTC 
TTCCAGCTGG AGTACGTGTT TAACAGCCCC TCGGGGGCAG ACAGCAATGC AGCTTCACTC 
TCCTCCTTGT CCAGCCTGGC ATGTATGCAC ATGGTTCGCT GGGCTGTCTG GAACCCCTCG 
CTGGAAGCCA GCTCCGGGCA GGTGATGTTA CCTCTCCAGG GTGGCATCCA GTCCAACCCT 
TCGCACTGTC TGGTCTATAA GGTACCCCCA GCCAGCATAA GCTCCGAAGA GGTGAAGCAG 
GTGGAGTCAG GGACGGTCCA GTTCCAGTAC TCGCTGAGCC CTGAAGAACA CCCAGGCACA 
CCCACAGCGA ATGCAAGCAG CCCCAAGGCA GAGCGGCGGT CTTTTAGGAA GCCACCCGTG 
TCCCCTTCAA GTCCACCAGC ATCGCCGTCC CAGGGGCTCA CCGCTGCCCA GGACTCACCC 
ATGGGACCAG GGCTGTCACT TTCTCAGCTG GCGGCTTCCC CACCATCCCC AGCTCCCCGC 
CGCCGCTCGG CCAAACCTTC GTCACAGCCA CCTGGGAGCC CTGTGCCCTC CCTGGTCCCA 
GGGGAGTTCC CCTTGGAGGG CAGGATCTCT CACCTGGAGG CTGACCTGAG CCAGACAGCC 
TTAGCCCTGG GAACGTCCGC TGCTGACCAC TTGCAGGAAC TGCCTTTCAC ACCCGTGCAC 
GCCCCGATCA TTGCAGGAAC CCAGACCAGG AGCTCTGGAG GGAAGCTGTC ACGGGCATCC 
CTGGCGCTGC TGCAGTCCGC TGGCTTTCCC GAGATTCTGG ACGCCAACAA GCAACCAGCT 
GAGGCTGTGA GTCCTACAGA CCCCGTGAAG TTCAGCCCTC AGAAGGAAGA ATCAGACTGT 
CTACAAGGCA ATGAGATAGT GTTGCAATTT CTTGCCTTTA GCAGGGTGCC CCAGGATGAG 
CAGGCAGCGC CATGGCCACA GACTGTGTAC TTCACCTTCC AATTCTACCG CTTCCCACCC 
GAGACCACGC CACGGCTGCA GCTCGTGGAG CTGGCCGATG CTGGGAAGAC CAGCTCTGCC 
TCCCTGTCGC ACATCCTCGC CCAGATCCAG AAGGATGGCT CCTTTGATGC GGGGTCTCCG 
GGCTTTCAGC GGAAGTACCT GGTGGACCCC GGGTTCCTGA GACCCGGGGA GCAGCGCTGG 
TTCGCCCGCT ACCTGGCCAT GCAGACCCTG CAGATCGACG TCTGGGATGG GGACTCCCTG 
CTTCTCATTG GATCTGCTGC CGTTCAGCTG AAGCACCTTC TCCGCCAGGG GCGGCCGGCC 
GTGCAGGTCT CGCATGAGCT GGAGGTTGTG GCGACTGAAT ACGAGCAGGA CGCCATGGTG 
GTGAGTGGAG ACATGACCAA GTTTGGCTGC GTCAGACCCA TCGACGTCCA CACGGTGGTG 
AAGGGCCAGC TGCACTTGAC CTTGGCCAAC GTGGGTCACT TGTGTGAACA GAGAGTAAGG 
AGTCCCAGTT CCCTGCCACC TTCCAGGTCG AGGGTCATCT CAAATGATGG AGTTGGCAAC 
TTCATGGGAG GCAGCCTCCT GACACCCGGG GGCCCCAGAT CAGTGAAAAA TGTGGTCCAA 
GCTCAGAAGC TGGCTGATGT GGACAGCGAA CTGGCCGCCC TGCTCTTCAC ACAACCGAGA 
CCAGGGGCCC GGGGGCCCCA GGGCATCAAT CAAGAGGGGG ATGCCGTCCG CAGGCGCAAG 
CTGGAGCGCA TGAGGGCCGT GCGGCTGCGG GAATGTGGAT TGGAGCCGGG CCGCCGCGGG 
CTGGGCGCGC TGGCCCAGCA GAGCCTCCAT GCCCAGCACG CCCGAGACCT GCAGGTCATT 
GCCGCCTACC GTGAGCGCAC AAAGGCGGAG AGCATCGCCA GCGCACTGAG CACGGCCATC 
ACCACGCAGC ACACCATCCA CGCCACCCTA GGCGCTGCCG AGTTCTTCGA GTTCGCCCTG 
AGGAACCCCC ACAACGCGCA GCACATGGTG ACCATCGAGA TAGACAACCC CGAGCTCAGT 
CTCATCCTGG ATGGTCAGGA GTGGCGGTAC TTCAAGGACA TGGCTGACTT GCACACACCC 
CTGGAGGAGG ACATGTTCCA CCTGCGGGGC AGCCTGGCCC CCCAACTCTT CCTGCGACCT 
CGGGAGACTG CCCACATCCC CTTCAAGTTC CAGACTTTCT CTGTGGGCCC CTGGGCCTCG 
GCACAGGCCT CTGCTGAGCT GAGATCAGAG AAGGACATGG ATGCCGGATC ACCCTATAAG 
TCCAGCATGA TGCCCACCAA ACACGCCAAG GTCCTGTTCC GCGCGGGCAG CGGCAAGCCC 
CTGGCCGTGC TCTGCCTGAC GGTGGAGCTG CAGCCTCACG TGGTGGACCA GGTCTTCCGC 
TTCCACCACC CGGAGCTCAC CTTCCTGAAG AAAGCCATCC GCCTGCCACC CTGGCACACG 
CTTCCAGGTG CCCCTGTAGG GACTTCCAGT GAGGACCCCC CTTTCCATGT TCGATGTAGT 
GACCCGAACA TCATTTGTGA GACACAGAGT GTGGGCCCCG GGGAGCCCCG TGACGTGTTC 
CTGAAGGTGG CCAGTGGCCC TAGCCCAGAG GTCAAGGACT TCTTTGTCAC CATCTACGTA 
GACCGCTGGT TAGCAACACC TGTCCAGACC TGGCAGGTCC ACCTGCACTC GCTGCAGCGA 
GTGGACGTCT CCTGTATCAC TGGCCAGAGG ACCCGCCTGG CTCTCGTCCT CCGGGGGACG 
CAGACAGTTA GGAGAGTGCA GGCTTTCAGC TCACACCCCC AGGAGCTGAC GACGGACCCC 
AGAGGTGTGT TCGTGCTGCC TCCTCACGGG GTGCAGGACT TGCATGTGAG CGTCAGGCCC 
TGCAGGCCGG GCAGCCGCTT CGCGCACCTC CACCTGGTTG ACGTGGACGC CCACCAGCTG 
GTGGCCTCGT GGCTCCTGTG TCTCTCCTGC CGGCCGCCTC TCATCTCCAA GGCCTTCGAG 
ATAAAGCTGC CCGCGGGGAG CGGGAGGGGC GCCAATAAGC GCATCACCTA CACCAATCCC 
TACCCCTCGC GGAGGGCCTA CCGCCTGCAC AGCGACCACC CCCACCTGCT GCAGTTCAAG 
GAGGACGCCT TCCAGGTCGG GGGAGGTGAG ACCTATACCA TCGGCCTGCG GTTTGTGTCC 
CGTCAGAGAG CCGGGGAGGA AGAGATCCTG ATTTACATCA ACGACCACGA AGACAAAAAC 
GAGGAGACCT TTTGCGTGAA GGTCATCTAC CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPHP4 ( XM_015466844.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015322330.1 Bos taurus nephronophthisis 4 (NPHP4), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa27367
Clone ID Related Accession (Same CDS sequence)	XM_015475180.1 , XM_015466842.1 , XM_015466845.1 , XM_024976773.1 , XM_005217172.4 , XM_015475179.1 , XM_015466844.1 , XM_015466846.1 , XM_005217172.3 , XM_010813390.2 , XM_024976775.1 , XM_024976776.1 , XM_005217171.3 , XM_024976772.1 , XM_010813390.3 , XM_015466843.1 , XM_005217171.4 , XM_015475180.2 , XM_024976774.1
Accession Version	XM_015466844.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4296bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	nephrocystin-4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646324.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACGACT GGCACAGGAT CTTTGACCAA AACCTGCTGG TCCCTCCGCA CCCACAGAGA 
GCACGCCAGC CTCTGAAGGA ATCGACGGCG TTTCAGTGTG TCCTGAAGTG GCTTGATGGC 
CCGCTAATTA AGCAGGGGGT GCTGGAGCTG CTATCGGAGG TGGGCTGCCA CCTGCGCGTG 
TCTCTCTTCG ATGTCACCTA TCGGCACTTC TTCGGGAGGA CGTGGAGGAG CTCGCCGAGG 
CCTGCAGAGC CGCTCACCAG GCAGCCGGCC AGGATCGTCT TCAATGAGCC CTTGTATTTT 
CACACGTCCT TGAACCACCC TCATATTGTG GCCGTGGTGG AGGTGGTCGT TGAAGGCAGA 
AGACGAGACG GGACCCTCCA GACTCTGTCC TGTGGGTTTG GAATTCTTCG GATCTTCAGC 
CACAAGCCGG AATCTCCAAC TTCCACTTCC CAGGACAAAC GGCTGAGACT GTATCATGGG 
AGCCCGCGAG CCCTCCTCCA CCCGCTCCTT CAGGACCCCA TAGAACAGAA CAAGCACATG 
ACCCTCATTG AGAACTGCAG CCTGCAGTAC ACGCTGAGGC CACACCCGCT CCTGGAGCCC 
GTGTTCCACC TTCTTCCCGA GAACTTGCTG GTGTCCGGTC TGCAGCAGAT ACCTGGCCTC 
CTTCCAGCTC ACGGGGAATC AGGAGATGCC CTCCGGAAGC CCCGCCTGCA GAAGCCTCTG 
ACGTGCTACC TGGATGATCT GTTCTTCACG CTGTATCCCT CGCTGGAGAA GTTCGAGGAG 
GAGCTTCTGG AACTCGTGAT CAGCGATTGC TTCCGGGAGG CGGGCAGCCC GCTGGACGTC 
GGCACCCTGG AGATCCTGGA GCGGCGCCTG TGCGTGGGCG TGCACAATGG GTTGGGCTTC 
GTGCAGCGGC CGCAGGTCCT CGTGCTGGTG CCCGAGCTGG AGGTGGCCTT GACACGCTCT 
GCCAGCTTCA GCCGGAAGGT CGGCTCCTCT TCCAAGGCCA GCTCTGGAAA CCAGGCGCTG 
GTCTTGAGAA GCCGTCTCCG ACTCCCTGAG ATGGTCCGTC ACCCCGCGTT CGCCATTGTC 
TTCCAGCTGG AGTACGTGTT TAACAGCCCC TCGGGGGCAG ACAGCAATGC AGCTTCACTC 
TCCTCCTTGT CCAGCCTGGC ATGTATGCAC ATGGTTCGCT GGGCTGTCTG GAACCCCTCG 
CTGGAAGCCA GCTCCGGGCA GGTGATGTTA CCTCTCCAGG GTGGCATCCA GTCCAACCCT 
TCGCACTGTC TGGTCTATAA GGTACCCCCA GCCAGCATAA GCTCCGAAGA GGTGAAGCAG 
GTGGAGTCAG GGACGGTCCA GTTCCAGTAC TCGCTGAGCC CTGAAGAACA CCCAGGCACA 
CCCACAGCGA ATGCAAGCAG CCCCAAGGCA GAGCGGCGGT CTTTTAGGAA GCCACCCGTG 
TCCCCTTCAA GTCCACCAGC ATCGCCGTCC CAGGGGCTCA CCGCTGCCCA GGACTCACCC 
ATGGGACCAG GGCTGTCACT TTCTCAGCTG GCGGCTTCCC CACCATCCCC AGCTCCCCGC 
CGCCGCTCGG CCAAACCTTC GTCACAGCCA CCTGGGAGCC CTGTGCCCTC CCTGGTCCCA 
GGGGAGTTCC CCTTGGAGGG CAGGATCTCT CACCTGGAGG CTGACCTGAG CCAGACAGCC 
TTAGCCCTGG GAACGTCCGC TGCTGACCAC TTGCAGGAAC TGCCTTTCAC ACCCGTGCAC 
GCCCCGATCA TTGCAGGAAC CCAGACCAGG AGCTCTGGAG GGAAGCTGTC ACGGGCATCC 
CTGGCGCTGC TGCAGTCCGC TGGCTTTCCC GAGATTCTGG ACGCCAACAA GCAACCAGCT 
GAGGCTGTGA GTCCTACAGA CCCCGTGAAG TTCAGCCCTC AGAAGGAAGA ATCAGACTGT 
CTACAAGGCA ATGAGATAGT GTTGCAATTT CTTGCCTTTA GCAGGGTGCC CCAGGATGAG 
CAGGCAGCGC CATGGCCACA GACTGTGTAC TTCACCTTCC AATTCTACCG CTTCCCACCC 
GAGACCACGC CACGGCTGCA GCTCGTGGAG CTGGCCGATG CTGGGAAGAC CAGCTCTGCC 
TCCCTGTCGC ACATCCTCGC CCAGATCCAG AAGGATGGCT CCTTTGATGC GGGGTCTCCG 
GGCTTTCAGC GGAAGTACCT GGTGGACCCC GGGTTCCTGA GACCCGGGGA GCAGCGCTGG 
TTCGCCCGCT ACCTGGCCAT GCAGACCCTG CAGATCGACG TCTGGGATGG GGACTCCCTG 
CTTCTCATTG GATCTGCTGC CGTTCAGCTG AAGCACCTTC TCCGCCAGGG GCGGCCGGCC 
GTGCAGGTCT CGCATGAGCT GGAGGTTGTG GCGACTGAAT ACGAGCAGGA CGCCATGGTG 
GTGAGTGGAG ACATGACCAA GTTTGGCTGC GTCAGACCCA TCGACGTCCA CACGGTGGTG 
AAGGGCCAGC TGCACTTGAC CTTGGCCAAC GTGGGTCACT TGTGTGAACA GAGAGTAAGG 
AGTCCCAGTT CCCTGCCACC TTCCAGGTCG AGGGTCATCT CAAATGATGG AGTTGGCAAC 
TTCATGGGAG GCAGCCTCCT GACACCCGGG GGCCCCAGAT CAGTGAAAAA TGTGGTCCAA 
GCTCAGAAGC TGGCTGATGT GGACAGCGAA CTGGCCGCCC TGCTCTTCAC ACAACCGAGA 
CCAGGGGCCC GGGGGCCCCA GGGCATCAAT CAAGAGGGGG ATGCCGTCCG CAGGCGCAAG 
CTGGAGCGCA TGAGGGCCGT GCGGCTGCGG GAATGTGGAT TGGAGCCGGG CCGCCGCGGG 
CTGGGCGCGC TGGCCCAGCA GAGCCTCCAT GCCCAGCACG CCCGAGACCT GCAGGTCATT 
GCCGCCTACC GTGAGCGCAC AAAGGCGGAG AGCATCGCCA GCGCACTGAG CACGGCCATC 
ACCACGCAGC ACACCATCCA CGCCACCCTA GGCGCTGCCG AGTTCTTCGA GTTCGCCCTG 
AGGAACCCCC ACAACGCGCA GCACATGGTG ACCATCGAGA TAGACAACCC CGAGCTCAGT 
CTCATCCTGG ATGGTCAGGA GTGGCGGTAC TTCAAGGACA TGGCTGACTT GCACACACCC 
CTGGAGGAGG ACATGTTCCA CCTGCGGGGC AGCCTGGCCC CCCAACTCTT CCTGCGACCT 
CGGGAGACTG CCCACATCCC CTTCAAGTTC CAGACTTTCT CTGTGGGCCC CTGGGCCTCG 
GCACAGGCCT CTGCTGAGCT GAGATCAGAG AAGGACATGG ATGCCGGATC ACCCTATAAG 
TCCAGCATGA TGCCCACCAA ACACGCCAAG GTCCTGTTCC GCGCGGGCAG CGGCAAGCCC 
CTGGCCGTGC TCTGCCTGAC GGTGGAGCTG CAGCCTCACG TGGTGGACCA GGTCTTCCGC 
TTCCACCACC CGGAGCTCAC CTTCCTGAAG AAAGCCATCC GCCTGCCACC CTGGCACACG 
CTTCCAGGTG CCCCTGTAGG GACTTCCAGT GAGGACCCCC CTTTCCATGT TCGATGTAGT 
GACCCGAACA TCATTTGTGA GACACAGAGT GTGGGCCCCG GGGAGCCCCG TGACGTGTTC 
CTGAAGGTGG CCAGTGGCCC TAGCCCAGAG GTCAAGGACT TCTTTGTCAC CATCTACGTA 
GACCGCTGGT TAGCAACACC TGTCCAGACC TGGCAGGTCC ACCTGCACTC GCTGCAGCGA 
GTGGACGTCT CCTGTATCAC TGGCCAGAGG ACCCGCCTGG CTCTCGTCCT CCGGGGGACG 
CAGACAGTTA GGAGAGTGCA GGCTTTCAGC TCACACCCCC AGGAGCTGAC GACGGACCCC 
AGAGGTGTGT TCGTGCTGCC TCCTCACGGG GTGCAGGACT TGCATGTGAG CGTCAGGCCC 
TGCAGGCCGG GCAGCCGCTT CGCGCACCTC CACCTGGTTG ACGTGGACGC CCACCAGCTG 
GTGGCCTCGT GGCTCCTGTG TCTCTCCTGC CGGCCGCCTC TCATCTCCAA GGCCTTCGAG 
ATAAAGCTGC CCGCGGGGAG CGGGAGGGGC GCCAATAAGC GCATCACCTA CACCAATCCC 
TACCCCTCGC GGAGGGCCTA CCGCCTGCAC AGCGACCACC CCCACCTGCT GCAGTTCAAG 
GAGGACGCCT TCCAGGTCGG GGGAGGTGAG ACCTATACCA TCGGCCTGCG GTTTGTGTCC 
CGTCAGAGAG CCGGGGAGGA AGAGATCCTG ATTTACATCA ACGACCACGA AGACAAAAAC 
GAGGAGACCT TTTGCGTGAA GGTCATCTAC CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015322330.1
CDS	185..4480
Translation	MHDWHRIFDQNLLVPPHPQRARQPLKESTAFQCVLKWLDGPLIKQGVLELLSEVGCHLRVSLFDVTYRHFFGRTWRSSPRPAEPLTRQPARIVFNEPLYFHTSLNHPHIVAVVEVVVEGRRRDGTLQTLSCGFGILRIFSHKPESPTSTSQDKRLRLYHGSPRALLHPLLQDPIEQNKHMTLIENCSLQYTLRPHPLLEPVFHLLPENLLVSGLQQIPGLLPAHGESGDALRKPRLQKPLTCYLDDLFFTLYPSLEKFEEELLELVISDCFREAGSPLDVGTLEILERRLCVGVHNGLGFVQRPQVLVLVPELEVALTRSASFSRKVGSSSKASSGNQALVLRSRLRLPEMVRHPAFAIVFQLEYVFNSPSGADSNAASLSSLSSLACMHMVRWAVWNPSLEASSGQVMLPLQGGIQSNPSHCLVYKVPPASISSEEVKQVESGTVQFQYSLSPEEHPGTPTANASSPKAERRSFRKPPVSPSSPPASPSQGLTAAQDSPMGPGLSLSQLAASPPSPAPRRRSAKPSSQPPGSPVPSLVPGEFPLEGRISHLEADLSQTALALGTSAADHLQELPFTPVHAPIIAGTQTRSSGGKLSRASLALLQSAGFPEILDANKQPAEAVSPTDPVKFSPQKEESDCLQGNEIVLQFLAFSRVPQDEQAAPWPQTVYFTFQFYRFPPETTPRLQLVELADAGKTSSASLSHILAQIQKDGSFDAGSPGFQRKYLVDPGFLRPGEQRWFARYLAMQTLQIDVWDGDSLLLIGSAAVQLKHLLRQGRPAVQVSHELEVVATEYEQDAMVVSGDMTKFGCVRPIDVHTVVKGQLHLTLANVGHLCEQRVRSPSSLPPSRSRVISNDGVGNFMGGSLLTPGGPRSVKNVVQAQKLADVDSELAALLFTQPRPGARGPQGINQEGDAVRRRKLERMRAVRLRECGLEPGRRGLGALAQQSLHAQHARDLQVIAAYRERTKAESIASALSTAITTQHTIHATLGAAEFFEFALRNPHNAQHMVTIEIDNPELSLILDGQEWRYFKDMADLHTPLEEDMFHLRGSLAPQLFLRPRETAHIPFKFQTFSVGPWASAQASAELRSEKDMDAGSPYKSSMMPTKHAKVLFRAGSGKPLAVLCLTVELQPHVVDQVFRFHHPELTFLKKAIRLPPWHTLPGAPVGTSSEDPPFHVRCSDPNIICETQSVGPGEPRDVFLKVASGPSPEVKDFFVTIYVDRWLATPVQTWQVHLHSLQRVDVSCITGQRTRLALVLRGTQTVRRVQAFSSHPQELTTDPRGVFVLPPHGVQDLHVSVRPCRPGSRFAHLHLVDVDAHQLVASWLLCLSCRPPLISKAFEIKLPAGSGRGANKRITYTNPYPSRRAYRLHSDHPHLLQFKEDAFQVGGGETYTIGLRFVSRQRAGEEEILIYINDHEDKNEETFCVKVIYQ

Target ORF information:

RefSeq Version	XM_015466844.1
Organism	Bos taurus(cattle)
Definition	Bos taurus nephronophthisis 4 (NPHP4), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015466844.1

ORF Insert Sequence:

ATGCACGACT GGCACAGGAT CTTTGACCAA AACCTGCTGG TCCCTCCGCA CCCACAGAGA 
GCACGCCAGC CTCTGAAGGA ATCGACGGCG TTTCAGTGTG TCCTGAAGTG GCTTGATGGC 
CCGCTAATTA AGCAGGGGGT GCTGGAGCTG CTATCGGAGG TGGGCTGCCA CCTGCGCGTG 
TCTCTCTTCG ATGTCACCTA TCGGCACTTC TTCGGGAGGA CGTGGAGGAG CTCGCCGAGG 
CCTGCAGAGC CGCTCACCAG GCAGCCGGCC AGGATCGTCT TCAATGAGCC CTTGTATTTT 
CACACGTCCT TGAACCACCC TCATATTGTG GCCGTGGTGG AGGTGGTCGT TGAAGGCAGA 
AGACGAGACG GGACCCTCCA GACTCTGTCC TGTGGGTTTG GAATTCTTCG GATCTTCAGC 
CACAAGCCGG AATCTCCAAC TTCCACTTCC CAGGACAAAC GGCTGAGACT GTATCATGGG 
AGCCCGCGAG CCCTCCTCCA CCCGCTCCTT CAGGACCCCA TAGAACAGAA CAAGCACATG 
ACCCTCATTG AGAACTGCAG CCTGCAGTAC ACGCTGAGGC CACACCCGCT CCTGGAGCCC 
GTGTTCCACC TTCTTCCCGA GAACTTGCTG GTGTCCGGTC TGCAGCAGAT ACCTGGCCTC 
CTTCCAGCTC ACGGGGAATC AGGAGATGCC CTCCGGAAGC CCCGCCTGCA GAAGCCTCTG 
ACGTGCTACC TGGATGATCT GTTCTTCACG CTGTATCCCT CGCTGGAGAA GTTCGAGGAG 
GAGCTTCTGG AACTCGTGAT CAGCGATTGC TTCCGGGAGG CGGGCAGCCC GCTGGACGTC 
GGCACCCTGG AGATCCTGGA GCGGCGCCTG TGCGTGGGCG TGCACAATGG GTTGGGCTTC 
GTGCAGCGGC CGCAGGTCCT CGTGCTGGTG CCCGAGCTGG AGGTGGCCTT GACACGCTCT 
GCCAGCTTCA GCCGGAAGGT CGGCTCCTCT TCCAAGGCCA GCTCTGGAAA CCAGGCGCTG 
GTCTTGAGAA GCCGTCTCCG ACTCCCTGAG ATGGTCCGTC ACCCCGCGTT CGCCATTGTC 
TTCCAGCTGG AGTACGTGTT TAACAGCCCC TCGGGGGCAG ACAGCAATGC AGCTTCACTC 
TCCTCCTTGT CCAGCCTGGC ATGTATGCAC ATGGTTCGCT GGGCTGTCTG GAACCCCTCG 
CTGGAAGCCA GCTCCGGGCA GGTGATGTTA CCTCTCCAGG GTGGCATCCA GTCCAACCCT 
TCGCACTGTC TGGTCTATAA GGTACCCCCA GCCAGCATAA GCTCCGAAGA GGTGAAGCAG 
GTGGAGTCAG GGACGGTCCA GTTCCAGTAC TCGCTGAGCC CTGAAGAACA CCCAGGCACA 
CCCACAGCGA ATGCAAGCAG CCCCAAGGCA GAGCGGCGGT CTTTTAGGAA GCCACCCGTG 
TCCCCTTCAA GTCCACCAGC ATCGCCGTCC CAGGGGCTCA CCGCTGCCCA GGACTCACCC 
ATGGGACCAG GGCTGTCACT TTCTCAGCTG GCGGCTTCCC CACCATCCCC AGCTCCCCGC 
CGCCGCTCGG CCAAACCTTC GTCACAGCCA CCTGGGAGCC CTGTGCCCTC CCTGGTCCCA 
GGGGAGTTCC CCTTGGAGGG CAGGATCTCT CACCTGGAGG CTGACCTGAG CCAGACAGCC 
TTAGCCCTGG GAACGTCCGC TGCTGACCAC TTGCAGGAAC TGCCTTTCAC ACCCGTGCAC 
GCCCCGATCA TTGCAGGAAC CCAGACCAGG AGCTCTGGAG GGAAGCTGTC ACGGGCATCC 
CTGGCGCTGC TGCAGTCCGC TGGCTTTCCC GAGATTCTGG ACGCCAACAA GCAACCAGCT 
GAGGCTGTGA GTCCTACAGA CCCCGTGAAG TTCAGCCCTC AGAAGGAAGA ATCAGACTGT 
CTACAAGGCA ATGAGATAGT GTTGCAATTT CTTGCCTTTA GCAGGGTGCC CCAGGATGAG 
CAGGCAGCGC CATGGCCACA GACTGTGTAC TTCACCTTCC AATTCTACCG CTTCCCACCC 
GAGACCACGC CACGGCTGCA GCTCGTGGAG CTGGCCGATG CTGGGAAGAC CAGCTCTGCC 
TCCCTGTCGC ACATCCTCGC CCAGATCCAG AAGGATGGCT CCTTTGATGC GGGGTCTCCG 
GGCTTTCAGC GGAAGTACCT GGTGGACCCC GGGTTCCTGA GACCCGGGGA GCAGCGCTGG 
TTCGCCCGCT ACCTGGCCAT GCAGACCCTG CAGATCGACG TCTGGGATGG GGACTCCCTG 
CTTCTCATTG GATCTGCTGC CGTTCAGCTG AAGCACCTTC TCCGCCAGGG GCGGCCGGCC 
GTGCAGGTCT CGCATGAGCT GGAGGTTGTG GCGACTGAAT ACGAGCAGGA CGCCATGGTG 
GTGAGTGGAG ACATGACCAA GTTTGGCTGC GTCAGACCCA TCGACGTCCA CACGGTGGTG 
AAGGGCCAGC TGCACTTGAC CTTGGCCAAC GTGGGTCACT TGTGTGAACA GAGAGTAAGG 
AGTCCCAGTT CCCTGCCACC TTCCAGGTCG AGGGTCATCT CAAATGATGG AGTTGGCAAC 
TTCATGGGAG GCAGCCTCCT GACACCCGGG GGCCCCAGAT CAGTGAAAAA TGTGGTCCAA 
GCTCAGAAGC TGGCTGATGT GGACAGCGAA CTGGCCGCCC TGCTCTTCAC ACAACCGAGA 
CCAGGGGCCC GGGGGCCCCA GGGCATCAAT CAAGAGGGGG ATGCCGTCCG CAGGCGCAAG 
CTGGAGCGCA TGAGGGCCGT GCGGCTGCGG GAATGTGGAT TGGAGCCGGG CCGCCGCGGG 
CTGGGCGCGC TGGCCCAGCA GAGCCTCCAT GCCCAGCACG CCCGAGACCT GCAGGTCATT 
GCCGCCTACC GTGAGCGCAC AAAGGCGGAG AGCATCGCCA GCGCACTGAG CACGGCCATC 
ACCACGCAGC ACACCATCCA CGCCACCCTA GGCGCTGCCG AGTTCTTCGA GTTCGCCCTG 
AGGAACCCCC ACAACGCGCA GCACATGGTG ACCATCGAGA TAGACAACCC CGAGCTCAGT 
CTCATCCTGG ATGGTCAGGA GTGGCGGTAC TTCAAGGACA TGGCTGACTT GCACACACCC 
CTGGAGGAGG ACATGTTCCA CCTGCGGGGC AGCCTGGCCC CCCAACTCTT CCTGCGACCT 
CGGGAGACTG CCCACATCCC CTTCAAGTTC CAGACTTTCT CTGTGGGCCC CTGGGCCTCG 
GCACAGGCCT CTGCTGAGCT GAGATCAGAG AAGGACATGG ATGCCGGATC ACCCTATAAG 
TCCAGCATGA TGCCCACCAA ACACGCCAAG GTCCTGTTCC GCGCGGGCAG CGGCAAGCCC 
CTGGCCGTGC TCTGCCTGAC GGTGGAGCTG CAGCCTCACG TGGTGGACCA GGTCTTCCGC 
TTCCACCACC CGGAGCTCAC CTTCCTGAAG AAAGCCATCC GCCTGCCACC CTGGCACACG 
CTTCCAGGTG CCCCTGTAGG GACTTCCAGT GAGGACCCCC CTTTCCATGT TCGATGTAGT 
GACCCGAACA TCATTTGTGA GACACAGAGT GTGGGCCCCG GGGAGCCCCG TGACGTGTTC 
CTGAAGGTGG CCAGTGGCCC TAGCCCAGAG GTCAAGGACT TCTTTGTCAC CATCTACGTA 
GACCGCTGGT TAGCAACACC TGTCCAGACC TGGCAGGTCC ACCTGCACTC GCTGCAGCGA 
GTGGACGTCT CCTGTATCAC TGGCCAGAGG ACCCGCCTGG CTCTCGTCCT CCGGGGGACG 
CAGACAGTTA GGAGAGTGCA GGCTTTCAGC TCACACCCCC AGGAGCTGAC GACGGACCCC 
AGAGGTGTGT TCGTGCTGCC TCCTCACGGG GTGCAGGACT TGCATGTGAG CGTCAGGCCC 
TGCAGGCCGG GCAGCCGCTT CGCGCACCTC CACCTGGTTG ACGTGGACGC CCACCAGCTG 
GTGGCCTCGT GGCTCCTGTG TCTCTCCTGC CGGCCGCCTC TCATCTCCAA GGCCTTCGAG 
ATAAAGCTGC CCGCGGGGAG CGGGAGGGGC GCCAATAAGC GCATCACCTA CACCAATCCC 
TACCCCTCGC GGAGGGCCTA CCGCCTGCAC AGCGACCACC CCCACCTGCT GCAGTTCAAG 
GAGGACGCCT TCCAGGTCGG GGGAGGTGAG ACCTATACCA TCGGCCTGCG GTTTGTGTCC 
CGTCAGAGAG CCGGGGAGGA AGAGATCCTG ATTTACATCA ACGACCACGA AGACAAAAAC 
GAGGAGACCT TTTGCGTGAA GGTCATCTAC CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPHP4 ( XM_015466846.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015322332.1 Bos taurus nephronophthisis 4 (NPHP4), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OBa27367
Clone ID Related Accession (Same CDS sequence)	XM_015475180.1 , XM_015466842.1 , XM_015466845.1 , XM_024976773.1 , XM_005217172.4 , XM_015475179.1 , XM_015466844.1 , XM_015466846.1 , XM_005217172.3 , XM_010813390.2 , XM_024976775.1 , XM_024976776.1 , XM_005217171.3 , XM_024976772.1 , XM_010813390.3 , XM_015466843.1 , XM_005217171.4 , XM_015475180.2 , XM_024976774.1
Accession Version	XM_015466846.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4296bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	nephrocystin-4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646324.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACGACT GGCACAGGAT CTTTGACCAA AACCTGCTGG TCCCTCCGCA CCCACAGAGA 
GCACGCCAGC CTCTGAAGGA ATCGACGGCG TTTCAGTGTG TCCTGAAGTG GCTTGATGGC 
CCGCTAATTA AGCAGGGGGT GCTGGAGCTG CTATCGGAGG TGGGCTGCCA CCTGCGCGTG 
TCTCTCTTCG ATGTCACCTA TCGGCACTTC TTCGGGAGGA CGTGGAGGAG CTCGCCGAGG 
CCTGCAGAGC CGCTCACCAG GCAGCCGGCC AGGATCGTCT TCAATGAGCC CTTGTATTTT 
CACACGTCCT TGAACCACCC TCATATTGTG GCCGTGGTGG AGGTGGTCGT TGAAGGCAGA 
AGACGAGACG GGACCCTCCA GACTCTGTCC TGTGGGTTTG GAATTCTTCG GATCTTCAGC 
CACAAGCCGG AATCTCCAAC TTCCACTTCC CAGGACAAAC GGCTGAGACT GTATCATGGG 
AGCCCGCGAG CCCTCCTCCA CCCGCTCCTT CAGGACCCCA TAGAACAGAA CAAGCACATG 
ACCCTCATTG AGAACTGCAG CCTGCAGTAC ACGCTGAGGC CACACCCGCT CCTGGAGCCC 
GTGTTCCACC TTCTTCCCGA GAACTTGCTG GTGTCCGGTC TGCAGCAGAT ACCTGGCCTC 
CTTCCAGCTC ACGGGGAATC AGGAGATGCC CTCCGGAAGC CCCGCCTGCA GAAGCCTCTG 
ACGTGCTACC TGGATGATCT GTTCTTCACG CTGTATCCCT CGCTGGAGAA GTTCGAGGAG 
GAGCTTCTGG AACTCGTGAT CAGCGATTGC TTCCGGGAGG CGGGCAGCCC GCTGGACGTC 
GGCACCCTGG AGATCCTGGA GCGGCGCCTG TGCGTGGGCG TGCACAATGG GTTGGGCTTC 
GTGCAGCGGC CGCAGGTCCT CGTGCTGGTG CCCGAGCTGG AGGTGGCCTT GACACGCTCT 
GCCAGCTTCA GCCGGAAGGT CGGCTCCTCT TCCAAGGCCA GCTCTGGAAA CCAGGCGCTG 
GTCTTGAGAA GCCGTCTCCG ACTCCCTGAG ATGGTCCGTC ACCCCGCGTT CGCCATTGTC 
TTCCAGCTGG AGTACGTGTT TAACAGCCCC TCGGGGGCAG ACAGCAATGC AGCTTCACTC 
TCCTCCTTGT CCAGCCTGGC ATGTATGCAC ATGGTTCGCT GGGCTGTCTG GAACCCCTCG 
CTGGAAGCCA GCTCCGGGCA GGTGATGTTA CCTCTCCAGG GTGGCATCCA GTCCAACCCT 
TCGCACTGTC TGGTCTATAA GGTACCCCCA GCCAGCATAA GCTCCGAAGA GGTGAAGCAG 
GTGGAGTCAG GGACGGTCCA GTTCCAGTAC TCGCTGAGCC CTGAAGAACA CCCAGGCACA 
CCCACAGCGA ATGCAAGCAG CCCCAAGGCA GAGCGGCGGT CTTTTAGGAA GCCACCCGTG 
TCCCCTTCAA GTCCACCAGC ATCGCCGTCC CAGGGGCTCA CCGCTGCCCA GGACTCACCC 
ATGGGACCAG GGCTGTCACT TTCTCAGCTG GCGGCTTCCC CACCATCCCC AGCTCCCCGC 
CGCCGCTCGG CCAAACCTTC GTCACAGCCA CCTGGGAGCC CTGTGCCCTC CCTGGTCCCA 
GGGGAGTTCC CCTTGGAGGG CAGGATCTCT CACCTGGAGG CTGACCTGAG CCAGACAGCC 
TTAGCCCTGG GAACGTCCGC TGCTGACCAC TTGCAGGAAC TGCCTTTCAC ACCCGTGCAC 
GCCCCGATCA TTGCAGGAAC CCAGACCAGG AGCTCTGGAG GGAAGCTGTC ACGGGCATCC 
CTGGCGCTGC TGCAGTCCGC TGGCTTTCCC GAGATTCTGG ACGCCAACAA GCAACCAGCT 
GAGGCTGTGA GTCCTACAGA CCCCGTGAAG TTCAGCCCTC AGAAGGAAGA ATCAGACTGT 
CTACAAGGCA ATGAGATAGT GTTGCAATTT CTTGCCTTTA GCAGGGTGCC CCAGGATGAG 
CAGGCAGCGC CATGGCCACA GACTGTGTAC TTCACCTTCC AATTCTACCG CTTCCCACCC 
GAGACCACGC CACGGCTGCA GCTCGTGGAG CTGGCCGATG CTGGGAAGAC CAGCTCTGCC 
TCCCTGTCGC ACATCCTCGC CCAGATCCAG AAGGATGGCT CCTTTGATGC GGGGTCTCCG 
GGCTTTCAGC GGAAGTACCT GGTGGACCCC GGGTTCCTGA GACCCGGGGA GCAGCGCTGG 
TTCGCCCGCT ACCTGGCCAT GCAGACCCTG CAGATCGACG TCTGGGATGG GGACTCCCTG 
CTTCTCATTG GATCTGCTGC CGTTCAGCTG AAGCACCTTC TCCGCCAGGG GCGGCCGGCC 
GTGCAGGTCT CGCATGAGCT GGAGGTTGTG GCGACTGAAT ACGAGCAGGA CGCCATGGTG 
GTGAGTGGAG ACATGACCAA GTTTGGCTGC GTCAGACCCA TCGACGTCCA CACGGTGGTG 
AAGGGCCAGC TGCACTTGAC CTTGGCCAAC GTGGGTCACT TGTGTGAACA GAGAGTAAGG 
AGTCCCAGTT CCCTGCCACC TTCCAGGTCG AGGGTCATCT CAAATGATGG AGTTGGCAAC 
TTCATGGGAG GCAGCCTCCT GACACCCGGG GGCCCCAGAT CAGTGAAAAA TGTGGTCCAA 
GCTCAGAAGC TGGCTGATGT GGACAGCGAA CTGGCCGCCC TGCTCTTCAC ACAACCGAGA 
CCAGGGGCCC GGGGGCCCCA GGGCATCAAT CAAGAGGGGG ATGCCGTCCG CAGGCGCAAG 
CTGGAGCGCA TGAGGGCCGT GCGGCTGCGG GAATGTGGAT TGGAGCCGGG CCGCCGCGGG 
CTGGGCGCGC TGGCCCAGCA GAGCCTCCAT GCCCAGCACG CCCGAGACCT GCAGGTCATT 
GCCGCCTACC GTGAGCGCAC AAAGGCGGAG AGCATCGCCA GCGCACTGAG CACGGCCATC 
ACCACGCAGC ACACCATCCA CGCCACCCTA GGCGCTGCCG AGTTCTTCGA GTTCGCCCTG 
AGGAACCCCC ACAACGCGCA GCACATGGTG ACCATCGAGA TAGACAACCC CGAGCTCAGT 
CTCATCCTGG ATGGTCAGGA GTGGCGGTAC TTCAAGGACA TGGCTGACTT GCACACACCC 
CTGGAGGAGG ACATGTTCCA CCTGCGGGGC AGCCTGGCCC CCCAACTCTT CCTGCGACCT 
CGGGAGACTG CCCACATCCC CTTCAAGTTC CAGACTTTCT CTGTGGGCCC CTGGGCCTCG 
GCACAGGCCT CTGCTGAGCT GAGATCAGAG AAGGACATGG ATGCCGGATC ACCCTATAAG 
TCCAGCATGA TGCCCACCAA ACACGCCAAG GTCCTGTTCC GCGCGGGCAG CGGCAAGCCC 
CTGGCCGTGC TCTGCCTGAC GGTGGAGCTG CAGCCTCACG TGGTGGACCA GGTCTTCCGC 
TTCCACCACC CGGAGCTCAC CTTCCTGAAG AAAGCCATCC GCCTGCCACC CTGGCACACG 
CTTCCAGGTG CCCCTGTAGG GACTTCCAGT GAGGACCCCC CTTTCCATGT TCGATGTAGT 
GACCCGAACA TCATTTGTGA GACACAGAGT GTGGGCCCCG GGGAGCCCCG TGACGTGTTC 
CTGAAGGTGG CCAGTGGCCC TAGCCCAGAG GTCAAGGACT TCTTTGTCAC CATCTACGTA 
GACCGCTGGT TAGCAACACC TGTCCAGACC TGGCAGGTCC ACCTGCACTC GCTGCAGCGA 
GTGGACGTCT CCTGTATCAC TGGCCAGAGG ACCCGCCTGG CTCTCGTCCT CCGGGGGACG 
CAGACAGTTA GGAGAGTGCA GGCTTTCAGC TCACACCCCC AGGAGCTGAC GACGGACCCC 
AGAGGTGTGT TCGTGCTGCC TCCTCACGGG GTGCAGGACT TGCATGTGAG CGTCAGGCCC 
TGCAGGCCGG GCAGCCGCTT CGCGCACCTC CACCTGGTTG ACGTGGACGC CCACCAGCTG 
GTGGCCTCGT GGCTCCTGTG TCTCTCCTGC CGGCCGCCTC TCATCTCCAA GGCCTTCGAG 
ATAAAGCTGC CCGCGGGGAG CGGGAGGGGC GCCAATAAGC GCATCACCTA CACCAATCCC 
TACCCCTCGC GGAGGGCCTA CCGCCTGCAC AGCGACCACC CCCACCTGCT GCAGTTCAAG 
GAGGACGCCT TCCAGGTCGG GGGAGGTGAG ACCTATACCA TCGGCCTGCG GTTTGTGTCC 
CGTCAGAGAG CCGGGGAGGA AGAGATCCTG ATTTACATCA ACGACCACGA AGACAAAAAC 
GAGGAGACCT TTTGCGTGAA GGTCATCTAC CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015322332.1
CDS	631..4926
Translation	MHDWHRIFDQNLLVPPHPQRARQPLKESTAFQCVLKWLDGPLIKQGVLELLSEVGCHLRVSLFDVTYRHFFGRTWRSSPRPAEPLTRQPARIVFNEPLYFHTSLNHPHIVAVVEVVVEGRRRDGTLQTLSCGFGILRIFSHKPESPTSTSQDKRLRLYHGSPRALLHPLLQDPIEQNKHMTLIENCSLQYTLRPHPLLEPVFHLLPENLLVSGLQQIPGLLPAHGESGDALRKPRLQKPLTCYLDDLFFTLYPSLEKFEEELLELVISDCFREAGSPLDVGTLEILERRLCVGVHNGLGFVQRPQVLVLVPELEVALTRSASFSRKVGSSSKASSGNQALVLRSRLRLPEMVRHPAFAIVFQLEYVFNSPSGADSNAASLSSLSSLACMHMVRWAVWNPSLEASSGQVMLPLQGGIQSNPSHCLVYKVPPASISSEEVKQVESGTVQFQYSLSPEEHPGTPTANASSPKAERRSFRKPPVSPSSPPASPSQGLTAAQDSPMGPGLSLSQLAASPPSPAPRRRSAKPSSQPPGSPVPSLVPGEFPLEGRISHLEADLSQTALALGTSAADHLQELPFTPVHAPIIAGTQTRSSGGKLSRASLALLQSAGFPEILDANKQPAEAVSPTDPVKFSPQKEESDCLQGNEIVLQFLAFSRVPQDEQAAPWPQTVYFTFQFYRFPPETTPRLQLVELADAGKTSSASLSHILAQIQKDGSFDAGSPGFQRKYLVDPGFLRPGEQRWFARYLAMQTLQIDVWDGDSLLLIGSAAVQLKHLLRQGRPAVQVSHELEVVATEYEQDAMVVSGDMTKFGCVRPIDVHTVVKGQLHLTLANVGHLCEQRVRSPSSLPPSRSRVISNDGVGNFMGGSLLTPGGPRSVKNVVQAQKLADVDSELAALLFTQPRPGARGPQGINQEGDAVRRRKLERMRAVRLRECGLEPGRRGLGALAQQSLHAQHARDLQVIAAYRERTKAESIASALSTAITTQHTIHATLGAAEFFEFALRNPHNAQHMVTIEIDNPELSLILDGQEWRYFKDMADLHTPLEEDMFHLRGSLAPQLFLRPRETAHIPFKFQTFSVGPWASAQASAELRSEKDMDAGSPYKSSMMPTKHAKVLFRAGSGKPLAVLCLTVELQPHVVDQVFRFHHPELTFLKKAIRLPPWHTLPGAPVGTSSEDPPFHVRCSDPNIICETQSVGPGEPRDVFLKVASGPSPEVKDFFVTIYVDRWLATPVQTWQVHLHSLQRVDVSCITGQRTRLALVLRGTQTVRRVQAFSSHPQELTTDPRGVFVLPPHGVQDLHVSVRPCRPGSRFAHLHLVDVDAHQLVASWLLCLSCRPPLISKAFEIKLPAGSGRGANKRITYTNPYPSRRAYRLHSDHPHLLQFKEDAFQVGGGETYTIGLRFVSRQRAGEEEILIYINDHEDKNEETFCVKVIYQ

Target ORF information:

RefSeq Version	XM_015466846.1
Organism	Bos taurus(cattle)
Definition	Bos taurus nephronophthisis 4 (NPHP4), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015466846.1

ORF Insert Sequence:

ATGCACGACT GGCACAGGAT CTTTGACCAA AACCTGCTGG TCCCTCCGCA CCCACAGAGA 
GCACGCCAGC CTCTGAAGGA ATCGACGGCG TTTCAGTGTG TCCTGAAGTG GCTTGATGGC 
CCGCTAATTA AGCAGGGGGT GCTGGAGCTG CTATCGGAGG TGGGCTGCCA CCTGCGCGTG 
TCTCTCTTCG ATGTCACCTA TCGGCACTTC TTCGGGAGGA CGTGGAGGAG CTCGCCGAGG 
CCTGCAGAGC CGCTCACCAG GCAGCCGGCC AGGATCGTCT TCAATGAGCC CTTGTATTTT 
CACACGTCCT TGAACCACCC TCATATTGTG GCCGTGGTGG AGGTGGTCGT TGAAGGCAGA 
AGACGAGACG GGACCCTCCA GACTCTGTCC TGTGGGTTTG GAATTCTTCG GATCTTCAGC 
CACAAGCCGG AATCTCCAAC TTCCACTTCC CAGGACAAAC GGCTGAGACT GTATCATGGG 
AGCCCGCGAG CCCTCCTCCA CCCGCTCCTT CAGGACCCCA TAGAACAGAA CAAGCACATG 
ACCCTCATTG AGAACTGCAG CCTGCAGTAC ACGCTGAGGC CACACCCGCT CCTGGAGCCC 
GTGTTCCACC TTCTTCCCGA GAACTTGCTG GTGTCCGGTC TGCAGCAGAT ACCTGGCCTC 
CTTCCAGCTC ACGGGGAATC AGGAGATGCC CTCCGGAAGC CCCGCCTGCA GAAGCCTCTG 
ACGTGCTACC TGGATGATCT GTTCTTCACG CTGTATCCCT CGCTGGAGAA GTTCGAGGAG 
GAGCTTCTGG AACTCGTGAT CAGCGATTGC TTCCGGGAGG CGGGCAGCCC GCTGGACGTC 
GGCACCCTGG AGATCCTGGA GCGGCGCCTG TGCGTGGGCG TGCACAATGG GTTGGGCTTC 
GTGCAGCGGC CGCAGGTCCT CGTGCTGGTG CCCGAGCTGG AGGTGGCCTT GACACGCTCT 
GCCAGCTTCA GCCGGAAGGT CGGCTCCTCT TCCAAGGCCA GCTCTGGAAA CCAGGCGCTG 
GTCTTGAGAA GCCGTCTCCG ACTCCCTGAG ATGGTCCGTC ACCCCGCGTT CGCCATTGTC 
TTCCAGCTGG AGTACGTGTT TAACAGCCCC TCGGGGGCAG ACAGCAATGC AGCTTCACTC 
TCCTCCTTGT CCAGCCTGGC ATGTATGCAC ATGGTTCGCT GGGCTGTCTG GAACCCCTCG 
CTGGAAGCCA GCTCCGGGCA GGTGATGTTA CCTCTCCAGG GTGGCATCCA GTCCAACCCT 
TCGCACTGTC TGGTCTATAA GGTACCCCCA GCCAGCATAA GCTCCGAAGA GGTGAAGCAG 
GTGGAGTCAG GGACGGTCCA GTTCCAGTAC TCGCTGAGCC CTGAAGAACA CCCAGGCACA 
CCCACAGCGA ATGCAAGCAG CCCCAAGGCA GAGCGGCGGT CTTTTAGGAA GCCACCCGTG 
TCCCCTTCAA GTCCACCAGC ATCGCCGTCC CAGGGGCTCA CCGCTGCCCA GGACTCACCC 
ATGGGACCAG GGCTGTCACT TTCTCAGCTG GCGGCTTCCC CACCATCCCC AGCTCCCCGC 
CGCCGCTCGG CCAAACCTTC GTCACAGCCA CCTGGGAGCC CTGTGCCCTC CCTGGTCCCA 
GGGGAGTTCC CCTTGGAGGG CAGGATCTCT CACCTGGAGG CTGACCTGAG CCAGACAGCC 
TTAGCCCTGG GAACGTCCGC TGCTGACCAC TTGCAGGAAC TGCCTTTCAC ACCCGTGCAC 
GCCCCGATCA TTGCAGGAAC CCAGACCAGG AGCTCTGGAG GGAAGCTGTC ACGGGCATCC 
CTGGCGCTGC TGCAGTCCGC TGGCTTTCCC GAGATTCTGG ACGCCAACAA GCAACCAGCT 
GAGGCTGTGA GTCCTACAGA CCCCGTGAAG TTCAGCCCTC AGAAGGAAGA ATCAGACTGT 
CTACAAGGCA ATGAGATAGT GTTGCAATTT CTTGCCTTTA GCAGGGTGCC CCAGGATGAG 
CAGGCAGCGC CATGGCCACA GACTGTGTAC TTCACCTTCC AATTCTACCG CTTCCCACCC 
GAGACCACGC CACGGCTGCA GCTCGTGGAG CTGGCCGATG CTGGGAAGAC CAGCTCTGCC 
TCCCTGTCGC ACATCCTCGC CCAGATCCAG AAGGATGGCT CCTTTGATGC GGGGTCTCCG 
GGCTTTCAGC GGAAGTACCT GGTGGACCCC GGGTTCCTGA GACCCGGGGA GCAGCGCTGG 
TTCGCCCGCT ACCTGGCCAT GCAGACCCTG CAGATCGACG TCTGGGATGG GGACTCCCTG 
CTTCTCATTG GATCTGCTGC CGTTCAGCTG AAGCACCTTC TCCGCCAGGG GCGGCCGGCC 
GTGCAGGTCT CGCATGAGCT GGAGGTTGTG GCGACTGAAT ACGAGCAGGA CGCCATGGTG 
GTGAGTGGAG ACATGACCAA GTTTGGCTGC GTCAGACCCA TCGACGTCCA CACGGTGGTG 
AAGGGCCAGC TGCACTTGAC CTTGGCCAAC GTGGGTCACT TGTGTGAACA GAGAGTAAGG 
AGTCCCAGTT CCCTGCCACC TTCCAGGTCG AGGGTCATCT CAAATGATGG AGTTGGCAAC 
TTCATGGGAG GCAGCCTCCT GACACCCGGG GGCCCCAGAT CAGTGAAAAA TGTGGTCCAA 
GCTCAGAAGC TGGCTGATGT GGACAGCGAA CTGGCCGCCC TGCTCTTCAC ACAACCGAGA 
CCAGGGGCCC GGGGGCCCCA GGGCATCAAT CAAGAGGGGG ATGCCGTCCG CAGGCGCAAG 
CTGGAGCGCA TGAGGGCCGT GCGGCTGCGG GAATGTGGAT TGGAGCCGGG CCGCCGCGGG 
CTGGGCGCGC TGGCCCAGCA GAGCCTCCAT GCCCAGCACG CCCGAGACCT GCAGGTCATT 
GCCGCCTACC GTGAGCGCAC AAAGGCGGAG AGCATCGCCA GCGCACTGAG CACGGCCATC 
ACCACGCAGC ACACCATCCA CGCCACCCTA GGCGCTGCCG AGTTCTTCGA GTTCGCCCTG 
AGGAACCCCC ACAACGCGCA GCACATGGTG ACCATCGAGA TAGACAACCC CGAGCTCAGT 
CTCATCCTGG ATGGTCAGGA GTGGCGGTAC TTCAAGGACA TGGCTGACTT GCACACACCC 
CTGGAGGAGG ACATGTTCCA CCTGCGGGGC AGCCTGGCCC CCCAACTCTT CCTGCGACCT 
CGGGAGACTG CCCACATCCC CTTCAAGTTC CAGACTTTCT CTGTGGGCCC CTGGGCCTCG 
GCACAGGCCT CTGCTGAGCT GAGATCAGAG AAGGACATGG ATGCCGGATC ACCCTATAAG 
TCCAGCATGA TGCCCACCAA ACACGCCAAG GTCCTGTTCC GCGCGGGCAG CGGCAAGCCC 
CTGGCCGTGC TCTGCCTGAC GGTGGAGCTG CAGCCTCACG TGGTGGACCA GGTCTTCCGC 
TTCCACCACC CGGAGCTCAC CTTCCTGAAG AAAGCCATCC GCCTGCCACC CTGGCACACG 
CTTCCAGGTG CCCCTGTAGG GACTTCCAGT GAGGACCCCC CTTTCCATGT TCGATGTAGT 
GACCCGAACA TCATTTGTGA GACACAGAGT GTGGGCCCCG GGGAGCCCCG TGACGTGTTC 
CTGAAGGTGG CCAGTGGCCC TAGCCCAGAG GTCAAGGACT TCTTTGTCAC CATCTACGTA 
GACCGCTGGT TAGCAACACC TGTCCAGACC TGGCAGGTCC ACCTGCACTC GCTGCAGCGA 
GTGGACGTCT CCTGTATCAC TGGCCAGAGG ACCCGCCTGG CTCTCGTCCT CCGGGGGACG 
CAGACAGTTA GGAGAGTGCA GGCTTTCAGC TCACACCCCC AGGAGCTGAC GACGGACCCC 
AGAGGTGTGT TCGTGCTGCC TCCTCACGGG GTGCAGGACT TGCATGTGAG CGTCAGGCCC 
TGCAGGCCGG GCAGCCGCTT CGCGCACCTC CACCTGGTTG ACGTGGACGC CCACCAGCTG 
GTGGCCTCGT GGCTCCTGTG TCTCTCCTGC CGGCCGCCTC TCATCTCCAA GGCCTTCGAG 
ATAAAGCTGC CCGCGGGGAG CGGGAGGGGC GCCAATAAGC GCATCACCTA CACCAATCCC 
TACCCCTCGC GGAGGGCCTA CCGCCTGCAC AGCGACCACC CCCACCTGCT GCAGTTCAAG 
GAGGACGCCT TCCAGGTCGG GGGAGGTGAG ACCTATACCA TCGGCCTGCG GTTTGTGTCC 
CGTCAGAGAG CCGGGGAGGA AGAGATCCTG ATTTACATCA ACGACCACGA AGACAAAAAC 
GAGGAGACCT TTTGCGTGAA GGTCATCTAC CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPHP4 ( XM_005217172.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005217229.1 Bos taurus nephronophthisis 4 (NPHP4), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBa27367
Clone ID Related Accession (Same CDS sequence)	XM_015475180.1 , XM_015466842.1 , XM_015466845.1 , XM_024976773.1 , XM_005217172.4 , XM_015475179.1 , XM_015466844.1 , XM_015466846.1 , XM_005217172.3 , XM_010813390.2 , XM_024976775.1 , XM_024976776.1 , XM_005217171.3 , XM_024976772.1 , XM_010813390.3 , XM_015466843.1 , XM_005217171.4 , XM_015475180.2 , XM_024976774.1
Accession Version	XM_005217172.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4296bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	nephrocystin-4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104439.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005217172.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACGACT GGCACAGGAT CTTTGACCAA AACCTGCTGG TCCCTCCGCA CCCACAGAGA 
GCACGCCAGC CTCTGAAGGA ATCGACGGCG TTTCAGTGTG TCCTGAAGTG GCTTGATGGC 
CCGCTAATTA AGCAGGGGGT GCTGGAGCTG CTATCGGAGG TGGGCTGCCA CCTGCGCGTG 
TCTCTCTTCG ATGTCACCTA TCGGCACTTC TTCGGGAGGA CGTGGAGGAG CTCGCCGAGG 
CCTGCAGAGC CGCTCACCAG GCAGCCGGCC AGGATCGTCT TCAATGAGCC CTTGTATTTT 
CACACGTCCT TGAACCACCC TCATATTGTG GCCGTGGTGG AGGTGGTCGT TGAAGGCAGA 
AGACGAGACG GGACCCTCCA GACTCTGTCC TGTGGGTTTG GAATTCTTCG GATCTTCAGC 
CACAAGCCGG AATCTCCAAC TTCCACTTCC CAGGACAAAC GGCTGAGACT GTATCATGGG 
AGCCCGCGAG CCCTCCTCCA CCCGCTCCTT CAGGACCCCA TAGAACAGAA CAAGCACATG 
ACCCTCATTG AGAACTGCAG CCTGCAGTAC ACGCTGAGGC CACACCCGCT CCTGGAGCCC 
GTGTTCCACC TTCTTCCCGA GAACTTGCTG GTGTCCGGTC TGCAGCAGAT ACCTGGCCTC 
CTTCCAGCTC ACGGGGAATC AGGAGATGCC CTCCGGAAGC CCCGCCTGCA GAAGCCTCTG 
ACGTGCTACC TGGATGATCT GTTCTTCACG CTGTATCCCT CGCTGGAGAA GTTCGAGGAG 
GAGCTTCTGG AACTCGTGAT CAGCGATTGC TTCCGGGAGG CGGGCAGCCC GCTGGACGTC 
GGCACCCTGG AGATCCTGGA GCGGCGCCTG TGCGTGGGCG TGCACAATGG GTTGGGCTTC 
GTGCAGCGGC CGCAGGTCCT CGTGCTGGTG CCCGAGCTGG AGGTGGCCTT GACACGCTCT 
GCCAGCTTCA GCCGGAAGGT CGGCTCCTCT TCCAAGGCCA GCTCTGGAAA CCAGGCGCTG 
GTCTTGAGAA GCCGTCTCCG ACTCCCTGAG ATGGTCCGTC ACCCCGCGTT CGCCATTGTC 
TTCCAGCTGG AGTACGTGTT TAACAGCCCC TCGGGGGCAG ACAGCAATGC AGCTTCACTC 
TCCTCCTTGT CCAGCCTGGC ATGTATGCAC ATGGTTCGCT GGGCTGTCTG GAACCCCTCG 
CTGGAAGCCA GCTCCGGGCA GGTGATGTTA CCTCTCCAGG GTGGCATCCA GTCCAACCCT 
TCGCACTGTC TGGTCTATAA GGTACCCCCA GCCAGCATAA GCTCCGAAGA GGTGAAGCAG 
GTGGAGTCAG GGACGGTCCA GTTCCAGTAC TCGCTGAGCC CTGAAGAACA CCCAGGCACA 
CCCACAGCGA ATGCAAGCAG CCCCAAGGCA GAGCGGCGGT CTTTTAGGAA GCCACCCGTG 
TCCCCTTCAA GTCCACCAGC ATCGCCGTCC CAGGGGCTCA CCGCTGCCCA GGACTCACCC 
ATGGGACCAG GGCTGTCACT TTCTCAGCTG GCGGCTTCCC CACCATCCCC AGCTCCCCGC 
CGCCGCTCGG CCAAACCTTC GTCACAGCCA CCTGGGAGCC CTGTGCCCTC CCTGGTCCCA 
GGGGAGTTCC CCTTGGAGGG CAGGATCTCT CACCTGGAGG CTGACCTGAG CCAGACAGCC 
TTAGCCCTGG GAACGTCCGC TGCTGACCAC TTGCAGGAAC TGCCTTTCAC ACCCGTGCAC 
GCCCCGATCA TTGCAGGAAC CCAGACCAGG AGCTCTGGAG GGAAGCTGTC ACGGGCATCC 
CTGGCGCTGC TGCAGTCCGC TGGCTTTCCC GAGATTCTGG ACGCCAACAA GCAACCAGCT 
GAGGCTGTGA GTCCTACAGA CCCCGTGAAG TTCAGCCCTC AGAAGGAAGA ATCAGACTGT 
CTACAAGGCA ATGAGATAGT GTTGCAATTT CTTGCCTTTA GCAGGGTGCC CCAGGATGAG 
CAGGCAGCGC CATGGCCACA GACTGTGTAC TTCACCTTCC AATTCTACCG CTTCCCACCC 
GAGACCACGC CACGGCTGCA GCTCGTGGAG CTGGCCGATG CTGGGAAGAC CAGCTCTGCC 
TCCCTGTCGC ACATCCTCGC CCAGATCCAG AAGGATGGCT CCTTTGATGC GGGGTCTCCG 
GGCTTTCAGC GGAAGTACCT GGTGGACCCC GGGTTCCTGA GACCCGGGGA GCAGCGCTGG 
TTCGCCCGCT ACCTGGCCAT GCAGACCCTG CAGATCGACG TCTGGGATGG GGACTCCCTG 
CTTCTCATTG GATCTGCTGC CGTTCAGCTG AAGCACCTTC TCCGCCAGGG GCGGCCGGCC 
GTGCAGGTCT CGCATGAGCT GGAGGTTGTG GCGACTGAAT ACGAGCAGGA CGCCATGGTG 
GTGAGTGGAG ACATGACCAA GTTTGGCTGC GTCAGACCCA TCGACGTCCA CACGGTGGTG 
AAGGGCCAGC TGCACTTGAC CTTGGCCAAC GTGGGTCACT TGTGTGAACA GAGAGTAAGG 
AGTCCCAGTT CCCTGCCACC TTCCAGGTCG AGGGTCATCT CAAATGATGG AGTTGGCAAC 
TTCATGGGAG GCAGCCTCCT GACACCCGGG GGCCCCAGAT CAGTGAAAAA TGTGGTCCAA 
GCTCAGAAGC TGGCTGATGT GGACAGCGAA CTGGCCGCCC TGCTCTTCAC ACAACCGAGA 
CCAGGGGCCC GGGGGCCCCA GGGCATCAAT CAAGAGGGGG ATGCCGTCCG CAGGCGCAAG 
CTGGAGCGCA TGAGGGCCGT GCGGCTGCGG GAATGTGGAT TGGAGCCGGG CCGCCGCGGG 
CTGGGCGCGC TGGCCCAGCA GAGCCTCCAT GCCCAGCACG CCCGAGACCT GCAGGTCATT 
GCCGCCTACC GTGAGCGCAC AAAGGCGGAG AGCATCGCCA GCGCACTGAG CACGGCCATC 
ACCACGCAGC ACACCATCCA CGCCACCCTA GGCGCTGCCG AGTTCTTCGA GTTCGCCCTG 
AGGAACCCCC ACAACGCGCA GCACATGGTG ACCATCGAGA TAGACAACCC CGAGCTCAGT 
CTCATCCTGG ATGGTCAGGA GTGGCGGTAC TTCAAGGACA TGGCTGACTT GCACACACCC 
CTGGAGGAGG ACATGTTCCA CCTGCGGGGC AGCCTGGCCC CCCAACTCTT CCTGCGACCT 
CGGGAGACTG CCCACATCCC CTTCAAGTTC CAGACTTTCT CTGTGGGCCC CTGGGCCTCG 
GCACAGGCCT CTGCTGAGCT GAGATCAGAG AAGGACATGG ATGCCGGATC ACCCTATAAG 
TCCAGCATGA TGCCCACCAA ACACGCCAAG GTCCTGTTCC GCGCGGGCAG CGGCAAGCCC 
CTGGCCGTGC TCTGCCTGAC GGTGGAGCTG CAGCCTCACG TGGTGGACCA GGTCTTCCGC 
TTCCACCACC CGGAGCTCAC CTTCCTGAAG AAAGCCATCC GCCTGCCACC CTGGCACACG 
CTTCCAGGTG CCCCTGTAGG GACTTCCAGT GAGGACCCCC CTTTCCATGT TCGATGTAGT 
GACCCGAACA TCATTTGTGA GACACAGAGT GTGGGCCCCG GGGAGCCCCG TGACGTGTTC 
CTGAAGGTGG CCAGTGGCCC TAGCCCAGAG GTCAAGGACT TCTTTGTCAC CATCTACGTA 
GACCGCTGGT TAGCAACACC TGTCCAGACC TGGCAGGTCC ACCTGCACTC GCTGCAGCGA 
GTGGACGTCT CCTGTATCAC TGGCCAGAGG ACCCGCCTGG CTCTCGTCCT CCGGGGGACG 
CAGACAGTTA GGAGAGTGCA GGCTTTCAGC TCACACCCCC AGGAGCTGAC GACGGACCCC 
AGAGGTGTGT TCGTGCTGCC TCCTCACGGG GTGCAGGACT TGCATGTGAG CGTCAGGCCC 
TGCAGGCCGG GCAGCCGCTT CGCGCACCTC CACCTGGTTG ACGTGGACGC CCACCAGCTG 
GTGGCCTCGT GGCTCCTGTG TCTCTCCTGC CGGCCGCCTC TCATCTCCAA GGCCTTCGAG 
ATAAAGCTGC CCGCGGGGAG CGGGAGGGGC GCCAATAAGC GCATCACCTA CACCAATCCC 
TACCCCTCGC GGAGGGCCTA CCGCCTGCAC AGCGACCACC CCCACCTGCT GCAGTTCAAG 
GAGGACGCCT TCCAGGTCGG GGGAGGTGAG ACCTATACCA TCGGCCTGCG GTTTGTGTCC 
CGTCAGAGAG CCGGGGAGGA AGAGATCCTG ATTTACATCA ACGACCACGA AGACAAAAAC 
GAGGAGACCT TTTGCGTGAA GGTCATCTAC CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005217229.1
CDS	1645..5940
Translation	MHDWHRIFDQNLLVPPHPQRARQPLKESTAFQCVLKWLDGPLIKQGVLELLSEVGCHLRVSLFDVTYRHFFGRTWRSSPRPAEPLTRQPARIVFNEPLYFHTSLNHPHIVAVVEVVVEGRRRDGTLQTLSCGFGILRIFSHKPESPTSTSQDKRLRLYHGSPRALLHPLLQDPIEQNKHMTLIENCSLQYTLRPHPLLEPVFHLLPENLLVSGLQQIPGLLPAHGESGDALRKPRLQKPLTCYLDDLFFTLYPSLEKFEEELLELVISDCFREAGSPLDVGTLEILERRLCVGVHNGLGFVQRPQVLVLVPELEVALTRSASFSRKVGSSSKASSGNQALVLRSRLRLPEMVRHPAFAIVFQLEYVFNSPSGADSNAASLSSLSSLACMHMVRWAVWNPSLEASSGQVMLPLQGGIQSNPSHCLVYKVPPASISSEEVKQVESGTVQFQYSLSPEEHPGTPTANASSPKAERRSFRKPPVSPSSPPASPSQGLTAAQDSPMGPGLSLSQLAASPPSPAPRRRSAKPSSQPPGSPVPSLVPGEFPLEGRISHLEADLSQTALALGTSAADHLQELPFTPVHAPIIAGTQTRSSGGKLSRASLALLQSAGFPEILDANKQPAEAVSPTDPVKFSPQKEESDCLQGNEIVLQFLAFSRVPQDEQAAPWPQTVYFTFQFYRFPPETTPRLQLVELADAGKTSSASLSHILAQIQKDGSFDAGSPGFQRKYLVDPGFLRPGEQRWFARYLAMQTLQIDVWDGDSLLLIGSAAVQLKHLLRQGRPAVQVSHELEVVATEYEQDAMVVSGDMTKFGCVRPIDVHTVVKGQLHLTLANVGHLCEQRVRSPSSLPPSRSRVISNDGVGNFMGGSLLTPGGPRSVKNVVQAQKLADVDSELAALLFTQPRPGARGPQGINQEGDAVRRRKLERMRAVRLRECGLEPGRRGLGALAQQSLHAQHARDLQVIAAYRERTKAESIASALSTAITTQHTIHATLGAAEFFEFALRNPHNAQHMVTIEIDNPELSLILDGQEWRYFKDMADLHTPLEEDMFHLRGSLAPQLFLRPRETAHIPFKFQTFSVGPWASAQASAELRSEKDMDAGSPYKSSMMPTKHAKVLFRAGSGKPLAVLCLTVELQPHVVDQVFRFHHPELTFLKKAIRLPPWHTLPGAPVGTSSEDPPFHVRCSDPNIICETQSVGPGEPRDVFLKVASGPSPEVKDFFVTIYVDRWLATPVQTWQVHLHSLQRVDVSCITGQRTRLALVLRGTQTVRRVQAFSSHPQELTTDPRGVFVLPPHGVQDLHVSVRPCRPGSRFAHLHLVDVDAHQLVASWLLCLSCRPPLISKAFEIKLPAGSGRGANKRITYTNPYPSRRAYRLHSDHPHLLQFKEDAFQVGGGETYTIGLRFVSRQRAGEEEILIYINDHEDKNEETFCVKVIYQ

Target ORF information:

RefSeq Version	XM_005217172.3
Organism	Bos taurus(cattle)
Definition	Bos taurus nephronophthisis 4 (NPHP4), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005217172.3

ORF Insert Sequence:

ATGCACGACT GGCACAGGAT CTTTGACCAA AACCTGCTGG TCCCTCCGCA CCCACAGAGA 
GCACGCCAGC CTCTGAAGGA ATCGACGGCG TTTCAGTGTG TCCTGAAGTG GCTTGATGGC 
CCGCTAATTA AGCAGGGGGT GCTGGAGCTG CTATCGGAGG TGGGCTGCCA CCTGCGCGTG 
TCTCTCTTCG ATGTCACCTA TCGGCACTTC TTCGGGAGGA CGTGGAGGAG CTCGCCGAGG 
CCTGCAGAGC CGCTCACCAG GCAGCCGGCC AGGATCGTCT TCAATGAGCC CTTGTATTTT 
CACACGTCCT TGAACCACCC TCATATTGTG GCCGTGGTGG AGGTGGTCGT TGAAGGCAGA 
AGACGAGACG GGACCCTCCA GACTCTGTCC TGTGGGTTTG GAATTCTTCG GATCTTCAGC 
CACAAGCCGG AATCTCCAAC TTCCACTTCC CAGGACAAAC GGCTGAGACT GTATCATGGG 
AGCCCGCGAG CCCTCCTCCA CCCGCTCCTT CAGGACCCCA TAGAACAGAA CAAGCACATG 
ACCCTCATTG AGAACTGCAG CCTGCAGTAC ACGCTGAGGC CACACCCGCT CCTGGAGCCC 
GTGTTCCACC TTCTTCCCGA GAACTTGCTG GTGTCCGGTC TGCAGCAGAT ACCTGGCCTC 
CTTCCAGCTC ACGGGGAATC AGGAGATGCC CTCCGGAAGC CCCGCCTGCA GAAGCCTCTG 
ACGTGCTACC TGGATGATCT GTTCTTCACG CTGTATCCCT CGCTGGAGAA GTTCGAGGAG 
GAGCTTCTGG AACTCGTGAT CAGCGATTGC TTCCGGGAGG CGGGCAGCCC GCTGGACGTC 
GGCACCCTGG AGATCCTGGA GCGGCGCCTG TGCGTGGGCG TGCACAATGG GTTGGGCTTC 
GTGCAGCGGC CGCAGGTCCT CGTGCTGGTG CCCGAGCTGG AGGTGGCCTT GACACGCTCT 
GCCAGCTTCA GCCGGAAGGT CGGCTCCTCT TCCAAGGCCA GCTCTGGAAA CCAGGCGCTG 
GTCTTGAGAA GCCGTCTCCG ACTCCCTGAG ATGGTCCGTC ACCCCGCGTT CGCCATTGTC 
TTCCAGCTGG AGTACGTGTT TAACAGCCCC TCGGGGGCAG ACAGCAATGC AGCTTCACTC 
TCCTCCTTGT CCAGCCTGGC ATGTATGCAC ATGGTTCGCT GGGCTGTCTG GAACCCCTCG 
CTGGAAGCCA GCTCCGGGCA GGTGATGTTA CCTCTCCAGG GTGGCATCCA GTCCAACCCT 
TCGCACTGTC TGGTCTATAA GGTACCCCCA GCCAGCATAA GCTCCGAAGA GGTGAAGCAG 
GTGGAGTCAG GGACGGTCCA GTTCCAGTAC TCGCTGAGCC CTGAAGAACA CCCAGGCACA 
CCCACAGCGA ATGCAAGCAG CCCCAAGGCA GAGCGGCGGT CTTTTAGGAA GCCACCCGTG 
TCCCCTTCAA GTCCACCAGC ATCGCCGTCC CAGGGGCTCA CCGCTGCCCA GGACTCACCC 
ATGGGACCAG GGCTGTCACT TTCTCAGCTG GCGGCTTCCC CACCATCCCC AGCTCCCCGC 
CGCCGCTCGG CCAAACCTTC GTCACAGCCA CCTGGGAGCC CTGTGCCCTC CCTGGTCCCA 
GGGGAGTTCC CCTTGGAGGG CAGGATCTCT CACCTGGAGG CTGACCTGAG CCAGACAGCC 
TTAGCCCTGG GAACGTCCGC TGCTGACCAC TTGCAGGAAC TGCCTTTCAC ACCCGTGCAC 
GCCCCGATCA TTGCAGGAAC CCAGACCAGG AGCTCTGGAG GGAAGCTGTC ACGGGCATCC 
CTGGCGCTGC TGCAGTCCGC TGGCTTTCCC GAGATTCTGG ACGCCAACAA GCAACCAGCT 
GAGGCTGTGA GTCCTACAGA CCCCGTGAAG TTCAGCCCTC AGAAGGAAGA ATCAGACTGT 
CTACAAGGCA ATGAGATAGT GTTGCAATTT CTTGCCTTTA GCAGGGTGCC CCAGGATGAG 
CAGGCAGCGC CATGGCCACA GACTGTGTAC TTCACCTTCC AATTCTACCG CTTCCCACCC 
GAGACCACGC CACGGCTGCA GCTCGTGGAG CTGGCCGATG CTGGGAAGAC CAGCTCTGCC 
TCCCTGTCGC ACATCCTCGC CCAGATCCAG AAGGATGGCT CCTTTGATGC GGGGTCTCCG 
GGCTTTCAGC GGAAGTACCT GGTGGACCCC GGGTTCCTGA GACCCGGGGA GCAGCGCTGG 
TTCGCCCGCT ACCTGGCCAT GCAGACCCTG CAGATCGACG TCTGGGATGG GGACTCCCTG 
CTTCTCATTG GATCTGCTGC CGTTCAGCTG AAGCACCTTC TCCGCCAGGG GCGGCCGGCC 
GTGCAGGTCT CGCATGAGCT GGAGGTTGTG GCGACTGAAT ACGAGCAGGA CGCCATGGTG 
GTGAGTGGAG ACATGACCAA GTTTGGCTGC GTCAGACCCA TCGACGTCCA CACGGTGGTG 
AAGGGCCAGC TGCACTTGAC CTTGGCCAAC GTGGGTCACT TGTGTGAACA GAGAGTAAGG 
AGTCCCAGTT CCCTGCCACC TTCCAGGTCG AGGGTCATCT CAAATGATGG AGTTGGCAAC 
TTCATGGGAG GCAGCCTCCT GACACCCGGG GGCCCCAGAT CAGTGAAAAA TGTGGTCCAA 
GCTCAGAAGC TGGCTGATGT GGACAGCGAA CTGGCCGCCC TGCTCTTCAC ACAACCGAGA 
CCAGGGGCCC GGGGGCCCCA GGGCATCAAT CAAGAGGGGG ATGCCGTCCG CAGGCGCAAG 
CTGGAGCGCA TGAGGGCCGT GCGGCTGCGG GAATGTGGAT TGGAGCCGGG CCGCCGCGGG 
CTGGGCGCGC TGGCCCAGCA GAGCCTCCAT GCCCAGCACG CCCGAGACCT GCAGGTCATT 
GCCGCCTACC GTGAGCGCAC AAAGGCGGAG AGCATCGCCA GCGCACTGAG CACGGCCATC 
ACCACGCAGC ACACCATCCA CGCCACCCTA GGCGCTGCCG AGTTCTTCGA GTTCGCCCTG 
AGGAACCCCC ACAACGCGCA GCACATGGTG ACCATCGAGA TAGACAACCC CGAGCTCAGT 
CTCATCCTGG ATGGTCAGGA GTGGCGGTAC TTCAAGGACA TGGCTGACTT GCACACACCC 
CTGGAGGAGG ACATGTTCCA CCTGCGGGGC AGCCTGGCCC CCCAACTCTT CCTGCGACCT 
CGGGAGACTG CCCACATCCC CTTCAAGTTC CAGACTTTCT CTGTGGGCCC CTGGGCCTCG 
GCACAGGCCT CTGCTGAGCT GAGATCAGAG AAGGACATGG ATGCCGGATC ACCCTATAAG 
TCCAGCATGA TGCCCACCAA ACACGCCAAG GTCCTGTTCC GCGCGGGCAG CGGCAAGCCC 
CTGGCCGTGC TCTGCCTGAC GGTGGAGCTG CAGCCTCACG TGGTGGACCA GGTCTTCCGC 
TTCCACCACC CGGAGCTCAC CTTCCTGAAG AAAGCCATCC GCCTGCCACC CTGGCACACG 
CTTCCAGGTG CCCCTGTAGG GACTTCCAGT GAGGACCCCC CTTTCCATGT TCGATGTAGT 
GACCCGAACA TCATTTGTGA GACACAGAGT GTGGGCCCCG GGGAGCCCCG TGACGTGTTC 
CTGAAGGTGG CCAGTGGCCC TAGCCCAGAG GTCAAGGACT TCTTTGTCAC CATCTACGTA 
GACCGCTGGT TAGCAACACC TGTCCAGACC TGGCAGGTCC ACCTGCACTC GCTGCAGCGA 
GTGGACGTCT CCTGTATCAC TGGCCAGAGG ACCCGCCTGG CTCTCGTCCT CCGGGGGACG 
CAGACAGTTA GGAGAGTGCA GGCTTTCAGC TCACACCCCC AGGAGCTGAC GACGGACCCC 
AGAGGTGTGT TCGTGCTGCC TCCTCACGGG GTGCAGGACT TGCATGTGAG CGTCAGGCCC 
TGCAGGCCGG GCAGCCGCTT CGCGCACCTC CACCTGGTTG ACGTGGACGC CCACCAGCTG 
GTGGCCTCGT GGCTCCTGTG TCTCTCCTGC CGGCCGCCTC TCATCTCCAA GGCCTTCGAG 
ATAAAGCTGC CCGCGGGGAG CGGGAGGGGC GCCAATAAGC GCATCACCTA CACCAATCCC 
TACCCCTCGC GGAGGGCCTA CCGCCTGCAC AGCGACCACC CCCACCTGCT GCAGTTCAAG 
GAGGACGCCT TCCAGGTCGG GGGAGGTGAG ACCTATACCA TCGGCCTGCG GTTTGTGTCC 
CGTCAGAGAG CCGGGGAGGA AGAGATCCTG ATTTACATCA ACGACCACGA AGACAAAAAC 
GAGGAGACCT TTTGCGTGAA GGTCATCTAC CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPHP4 ( XM_010813390.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010811692.1 Bos taurus nephronophthisis 4 (NPHP4), transcript variant X7, mRNA.

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CloneID	OBa27367
Clone ID Related Accession (Same CDS sequence)	XM_015475180.1 , XM_015466842.1 , XM_015466845.1 , XM_024976773.1 , XM_005217172.4 , XM_015475179.1 , XM_015466844.1 , XM_015466846.1 , XM_005217172.3 , XM_010813390.2 , XM_024976775.1 , XM_024976776.1 , XM_005217171.3 , XM_024976772.1 , XM_010813390.3 , XM_015466843.1 , XM_005217171.4 , XM_015475180.2 , XM_024976774.1
Accession Version	XM_010813390.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4296bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	nephrocystin-4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104439.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010813390.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACGACT GGCACAGGAT CTTTGACCAA AACCTGCTGG TCCCTCCGCA CCCACAGAGA 
GCACGCCAGC CTCTGAAGGA ATCGACGGCG TTTCAGTGTG TCCTGAAGTG GCTTGATGGC 
CCGCTAATTA AGCAGGGGGT GCTGGAGCTG CTATCGGAGG TGGGCTGCCA CCTGCGCGTG 
TCTCTCTTCG ATGTCACCTA TCGGCACTTC TTCGGGAGGA CGTGGAGGAG CTCGCCGAGG 
CCTGCAGAGC CGCTCACCAG GCAGCCGGCC AGGATCGTCT TCAATGAGCC CTTGTATTTT 
CACACGTCCT TGAACCACCC TCATATTGTG GCCGTGGTGG AGGTGGTCGT TGAAGGCAGA 
AGACGAGACG GGACCCTCCA GACTCTGTCC TGTGGGTTTG GAATTCTTCG GATCTTCAGC 
CACAAGCCGG AATCTCCAAC TTCCACTTCC CAGGACAAAC GGCTGAGACT GTATCATGGG 
AGCCCGCGAG CCCTCCTCCA CCCGCTCCTT CAGGACCCCA TAGAACAGAA CAAGCACATG 
ACCCTCATTG AGAACTGCAG CCTGCAGTAC ACGCTGAGGC CACACCCGCT CCTGGAGCCC 
GTGTTCCACC TTCTTCCCGA GAACTTGCTG GTGTCCGGTC TGCAGCAGAT ACCTGGCCTC 
CTTCCAGCTC ACGGGGAATC AGGAGATGCC CTCCGGAAGC CCCGCCTGCA GAAGCCTCTG 
ACGTGCTACC TGGATGATCT GTTCTTCACG CTGTATCCCT CGCTGGAGAA GTTCGAGGAG 
GAGCTTCTGG AACTCGTGAT CAGCGATTGC TTCCGGGAGG CGGGCAGCCC GCTGGACGTC 
GGCACCCTGG AGATCCTGGA GCGGCGCCTG TGCGTGGGCG TGCACAATGG GTTGGGCTTC 
GTGCAGCGGC CGCAGGTCCT CGTGCTGGTG CCCGAGCTGG AGGTGGCCTT GACACGCTCT 
GCCAGCTTCA GCCGGAAGGT CGGCTCCTCT TCCAAGGCCA GCTCTGGAAA CCAGGCGCTG 
GTCTTGAGAA GCCGTCTCCG ACTCCCTGAG ATGGTCCGTC ACCCCGCGTT CGCCATTGTC 
TTCCAGCTGG AGTACGTGTT TAACAGCCCC TCGGGGGCAG ACAGCAATGC AGCTTCACTC 
TCCTCCTTGT CCAGCCTGGC ATGTATGCAC ATGGTTCGCT GGGCTGTCTG GAACCCCTCG 
CTGGAAGCCA GCTCCGGGCA GGTGATGTTA CCTCTCCAGG GTGGCATCCA GTCCAACCCT 
TCGCACTGTC TGGTCTATAA GGTACCCCCA GCCAGCATAA GCTCCGAAGA GGTGAAGCAG 
GTGGAGTCAG GGACGGTCCA GTTCCAGTAC TCGCTGAGCC CTGAAGAACA CCCAGGCACA 
CCCACAGCGA ATGCAAGCAG CCCCAAGGCA GAGCGGCGGT CTTTTAGGAA GCCACCCGTG 
TCCCCTTCAA GTCCACCAGC ATCGCCGTCC CAGGGGCTCA CCGCTGCCCA GGACTCACCC 
ATGGGACCAG GGCTGTCACT TTCTCAGCTG GCGGCTTCCC CACCATCCCC AGCTCCCCGC 
CGCCGCTCGG CCAAACCTTC GTCACAGCCA CCTGGGAGCC CTGTGCCCTC CCTGGTCCCA 
GGGGAGTTCC CCTTGGAGGG CAGGATCTCT CACCTGGAGG CTGACCTGAG CCAGACAGCC 
TTAGCCCTGG GAACGTCCGC TGCTGACCAC TTGCAGGAAC TGCCTTTCAC ACCCGTGCAC 
GCCCCGATCA TTGCAGGAAC CCAGACCAGG AGCTCTGGAG GGAAGCTGTC ACGGGCATCC 
CTGGCGCTGC TGCAGTCCGC TGGCTTTCCC GAGATTCTGG ACGCCAACAA GCAACCAGCT 
GAGGCTGTGA GTCCTACAGA CCCCGTGAAG TTCAGCCCTC AGAAGGAAGA ATCAGACTGT 
CTACAAGGCA ATGAGATAGT GTTGCAATTT CTTGCCTTTA GCAGGGTGCC CCAGGATGAG 
CAGGCAGCGC CATGGCCACA GACTGTGTAC TTCACCTTCC AATTCTACCG CTTCCCACCC 
GAGACCACGC CACGGCTGCA GCTCGTGGAG CTGGCCGATG CTGGGAAGAC CAGCTCTGCC 
TCCCTGTCGC ACATCCTCGC CCAGATCCAG AAGGATGGCT CCTTTGATGC GGGGTCTCCG 
GGCTTTCAGC GGAAGTACCT GGTGGACCCC GGGTTCCTGA GACCCGGGGA GCAGCGCTGG 
TTCGCCCGCT ACCTGGCCAT GCAGACCCTG CAGATCGACG TCTGGGATGG GGACTCCCTG 
CTTCTCATTG GATCTGCTGC CGTTCAGCTG AAGCACCTTC TCCGCCAGGG GCGGCCGGCC 
GTGCAGGTCT CGCATGAGCT GGAGGTTGTG GCGACTGAAT ACGAGCAGGA CGCCATGGTG 
GTGAGTGGAG ACATGACCAA GTTTGGCTGC GTCAGACCCA TCGACGTCCA CACGGTGGTG 
AAGGGCCAGC TGCACTTGAC CTTGGCCAAC GTGGGTCACT TGTGTGAACA GAGAGTAAGG 
AGTCCCAGTT CCCTGCCACC TTCCAGGTCG AGGGTCATCT CAAATGATGG AGTTGGCAAC 
TTCATGGGAG GCAGCCTCCT GACACCCGGG GGCCCCAGAT CAGTGAAAAA TGTGGTCCAA 
GCTCAGAAGC TGGCTGATGT GGACAGCGAA CTGGCCGCCC TGCTCTTCAC ACAACCGAGA 
CCAGGGGCCC GGGGGCCCCA GGGCATCAAT CAAGAGGGGG ATGCCGTCCG CAGGCGCAAG 
CTGGAGCGCA TGAGGGCCGT GCGGCTGCGG GAATGTGGAT TGGAGCCGGG CCGCCGCGGG 
CTGGGCGCGC TGGCCCAGCA GAGCCTCCAT GCCCAGCACG CCCGAGACCT GCAGGTCATT 
GCCGCCTACC GTGAGCGCAC AAAGGCGGAG AGCATCGCCA GCGCACTGAG CACGGCCATC 
ACCACGCAGC ACACCATCCA CGCCACCCTA GGCGCTGCCG AGTTCTTCGA GTTCGCCCTG 
AGGAACCCCC ACAACGCGCA GCACATGGTG ACCATCGAGA TAGACAACCC CGAGCTCAGT 
CTCATCCTGG ATGGTCAGGA GTGGCGGTAC TTCAAGGACA TGGCTGACTT GCACACACCC 
CTGGAGGAGG ACATGTTCCA CCTGCGGGGC AGCCTGGCCC CCCAACTCTT CCTGCGACCT 
CGGGAGACTG CCCACATCCC CTTCAAGTTC CAGACTTTCT CTGTGGGCCC CTGGGCCTCG 
GCACAGGCCT CTGCTGAGCT GAGATCAGAG AAGGACATGG ATGCCGGATC ACCCTATAAG 
TCCAGCATGA TGCCCACCAA ACACGCCAAG GTCCTGTTCC GCGCGGGCAG CGGCAAGCCC 
CTGGCCGTGC TCTGCCTGAC GGTGGAGCTG CAGCCTCACG TGGTGGACCA GGTCTTCCGC 
TTCCACCACC CGGAGCTCAC CTTCCTGAAG AAAGCCATCC GCCTGCCACC CTGGCACACG 
CTTCCAGGTG CCCCTGTAGG GACTTCCAGT GAGGACCCCC CTTTCCATGT TCGATGTAGT 
GACCCGAACA TCATTTGTGA GACACAGAGT GTGGGCCCCG GGGAGCCCCG TGACGTGTTC 
CTGAAGGTGG CCAGTGGCCC TAGCCCAGAG GTCAAGGACT TCTTTGTCAC CATCTACGTA 
GACCGCTGGT TAGCAACACC TGTCCAGACC TGGCAGGTCC ACCTGCACTC GCTGCAGCGA 
GTGGACGTCT CCTGTATCAC TGGCCAGAGG ACCCGCCTGG CTCTCGTCCT CCGGGGGACG 
CAGACAGTTA GGAGAGTGCA GGCTTTCAGC TCACACCCCC AGGAGCTGAC GACGGACCCC 
AGAGGTGTGT TCGTGCTGCC TCCTCACGGG GTGCAGGACT TGCATGTGAG CGTCAGGCCC 
TGCAGGCCGG GCAGCCGCTT CGCGCACCTC CACCTGGTTG ACGTGGACGC CCACCAGCTG 
GTGGCCTCGT GGCTCCTGTG TCTCTCCTGC CGGCCGCCTC TCATCTCCAA GGCCTTCGAG 
ATAAAGCTGC CCGCGGGGAG CGGGAGGGGC GCCAATAAGC GCATCACCTA CACCAATCCC 
TACCCCTCGC GGAGGGCCTA CCGCCTGCAC AGCGACCACC CCCACCTGCT GCAGTTCAAG 
GAGGACGCCT TCCAGGTCGG GGGAGGTGAG ACCTATACCA TCGGCCTGCG GTTTGTGTCC 
CGTCAGAGAG CCGGGGAGGA AGAGATCCTG ATTTACATCA ACGACCACGA AGACAAAAAC 
GAGGAGACCT TTTGCGTGAA GGTCATCTAC CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010811692.1
CDS	631..4926
Translation	MHDWHRIFDQNLLVPPHPQRARQPLKESTAFQCVLKWLDGPLIKQGVLELLSEVGCHLRVSLFDVTYRHFFGRTWRSSPRPAEPLTRQPARIVFNEPLYFHTSLNHPHIVAVVEVVVEGRRRDGTLQTLSCGFGILRIFSHKPESPTSTSQDKRLRLYHGSPRALLHPLLQDPIEQNKHMTLIENCSLQYTLRPHPLLEPVFHLLPENLLVSGLQQIPGLLPAHGESGDALRKPRLQKPLTCYLDDLFFTLYPSLEKFEEELLELVISDCFREAGSPLDVGTLEILERRLCVGVHNGLGFVQRPQVLVLVPELEVALTRSASFSRKVGSSSKASSGNQALVLRSRLRLPEMVRHPAFAIVFQLEYVFNSPSGADSNAASLSSLSSLACMHMVRWAVWNPSLEASSGQVMLPLQGGIQSNPSHCLVYKVPPASISSEEVKQVESGTVQFQYSLSPEEHPGTPTANASSPKAERRSFRKPPVSPSSPPASPSQGLTAAQDSPMGPGLSLSQLAASPPSPAPRRRSAKPSSQPPGSPVPSLVPGEFPLEGRISHLEADLSQTALALGTSAADHLQELPFTPVHAPIIAGTQTRSSGGKLSRASLALLQSAGFPEILDANKQPAEAVSPTDPVKFSPQKEESDCLQGNEIVLQFLAFSRVPQDEQAAPWPQTVYFTFQFYRFPPETTPRLQLVELADAGKTSSASLSHILAQIQKDGSFDAGSPGFQRKYLVDPGFLRPGEQRWFARYLAMQTLQIDVWDGDSLLLIGSAAVQLKHLLRQGRPAVQVSHELEVVATEYEQDAMVVSGDMTKFGCVRPIDVHTVVKGQLHLTLANVGHLCEQRVRSPSSLPPSRSRVISNDGVGNFMGGSLLTPGGPRSVKNVVQAQKLADVDSELAALLFTQPRPGARGPQGINQEGDAVRRRKLERMRAVRLRECGLEPGRRGLGALAQQSLHAQHARDLQVIAAYRERTKAESIASALSTAITTQHTIHATLGAAEFFEFALRNPHNAQHMVTIEIDNPELSLILDGQEWRYFKDMADLHTPLEEDMFHLRGSLAPQLFLRPRETAHIPFKFQTFSVGPWASAQASAELRSEKDMDAGSPYKSSMMPTKHAKVLFRAGSGKPLAVLCLTVELQPHVVDQVFRFHHPELTFLKKAIRLPPWHTLPGAPVGTSSEDPPFHVRCSDPNIICETQSVGPGEPRDVFLKVASGPSPEVKDFFVTIYVDRWLATPVQTWQVHLHSLQRVDVSCITGQRTRLALVLRGTQTVRRVQAFSSHPQELTTDPRGVFVLPPHGVQDLHVSVRPCRPGSRFAHLHLVDVDAHQLVASWLLCLSCRPPLISKAFEIKLPAGSGRGANKRITYTNPYPSRRAYRLHSDHPHLLQFKEDAFQVGGGETYTIGLRFVSRQRAGEEEILIYINDHEDKNEETFCVKVIYQ

Target ORF information:

RefSeq Version	XM_010813390.2
Organism	Bos taurus(cattle)
Definition	Bos taurus nephronophthisis 4 (NPHP4), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010813390.2

ORF Insert Sequence:

ATGCACGACT GGCACAGGAT CTTTGACCAA AACCTGCTGG TCCCTCCGCA CCCACAGAGA 
GCACGCCAGC CTCTGAAGGA ATCGACGGCG TTTCAGTGTG TCCTGAAGTG GCTTGATGGC 
CCGCTAATTA AGCAGGGGGT GCTGGAGCTG CTATCGGAGG TGGGCTGCCA CCTGCGCGTG 
TCTCTCTTCG ATGTCACCTA TCGGCACTTC TTCGGGAGGA CGTGGAGGAG CTCGCCGAGG 
CCTGCAGAGC CGCTCACCAG GCAGCCGGCC AGGATCGTCT TCAATGAGCC CTTGTATTTT 
CACACGTCCT TGAACCACCC TCATATTGTG GCCGTGGTGG AGGTGGTCGT TGAAGGCAGA 
AGACGAGACG GGACCCTCCA GACTCTGTCC TGTGGGTTTG GAATTCTTCG GATCTTCAGC 
CACAAGCCGG AATCTCCAAC TTCCACTTCC CAGGACAAAC GGCTGAGACT GTATCATGGG 
AGCCCGCGAG CCCTCCTCCA CCCGCTCCTT CAGGACCCCA TAGAACAGAA CAAGCACATG 
ACCCTCATTG AGAACTGCAG CCTGCAGTAC ACGCTGAGGC CACACCCGCT CCTGGAGCCC 
GTGTTCCACC TTCTTCCCGA GAACTTGCTG GTGTCCGGTC TGCAGCAGAT ACCTGGCCTC 
CTTCCAGCTC ACGGGGAATC AGGAGATGCC CTCCGGAAGC CCCGCCTGCA GAAGCCTCTG 
ACGTGCTACC TGGATGATCT GTTCTTCACG CTGTATCCCT CGCTGGAGAA GTTCGAGGAG 
GAGCTTCTGG AACTCGTGAT CAGCGATTGC TTCCGGGAGG CGGGCAGCCC GCTGGACGTC 
GGCACCCTGG AGATCCTGGA GCGGCGCCTG TGCGTGGGCG TGCACAATGG GTTGGGCTTC 
GTGCAGCGGC CGCAGGTCCT CGTGCTGGTG CCCGAGCTGG AGGTGGCCTT GACACGCTCT 
GCCAGCTTCA GCCGGAAGGT CGGCTCCTCT TCCAAGGCCA GCTCTGGAAA CCAGGCGCTG 
GTCTTGAGAA GCCGTCTCCG ACTCCCTGAG ATGGTCCGTC ACCCCGCGTT CGCCATTGTC 
TTCCAGCTGG AGTACGTGTT TAACAGCCCC TCGGGGGCAG ACAGCAATGC AGCTTCACTC 
TCCTCCTTGT CCAGCCTGGC ATGTATGCAC ATGGTTCGCT GGGCTGTCTG GAACCCCTCG 
CTGGAAGCCA GCTCCGGGCA GGTGATGTTA CCTCTCCAGG GTGGCATCCA GTCCAACCCT 
TCGCACTGTC TGGTCTATAA GGTACCCCCA GCCAGCATAA GCTCCGAAGA GGTGAAGCAG 
GTGGAGTCAG GGACGGTCCA GTTCCAGTAC TCGCTGAGCC CTGAAGAACA CCCAGGCACA 
CCCACAGCGA ATGCAAGCAG CCCCAAGGCA GAGCGGCGGT CTTTTAGGAA GCCACCCGTG 
TCCCCTTCAA GTCCACCAGC ATCGCCGTCC CAGGGGCTCA CCGCTGCCCA GGACTCACCC 
ATGGGACCAG GGCTGTCACT TTCTCAGCTG GCGGCTTCCC CACCATCCCC AGCTCCCCGC 
CGCCGCTCGG CCAAACCTTC GTCACAGCCA CCTGGGAGCC CTGTGCCCTC CCTGGTCCCA 
GGGGAGTTCC CCTTGGAGGG CAGGATCTCT CACCTGGAGG CTGACCTGAG CCAGACAGCC 
TTAGCCCTGG GAACGTCCGC TGCTGACCAC TTGCAGGAAC TGCCTTTCAC ACCCGTGCAC 
GCCCCGATCA TTGCAGGAAC CCAGACCAGG AGCTCTGGAG GGAAGCTGTC ACGGGCATCC 
CTGGCGCTGC TGCAGTCCGC TGGCTTTCCC GAGATTCTGG ACGCCAACAA GCAACCAGCT 
GAGGCTGTGA GTCCTACAGA CCCCGTGAAG TTCAGCCCTC AGAAGGAAGA ATCAGACTGT 
CTACAAGGCA ATGAGATAGT GTTGCAATTT CTTGCCTTTA GCAGGGTGCC CCAGGATGAG 
CAGGCAGCGC CATGGCCACA GACTGTGTAC TTCACCTTCC AATTCTACCG CTTCCCACCC 
GAGACCACGC CACGGCTGCA GCTCGTGGAG CTGGCCGATG CTGGGAAGAC CAGCTCTGCC 
TCCCTGTCGC ACATCCTCGC CCAGATCCAG AAGGATGGCT CCTTTGATGC GGGGTCTCCG 
GGCTTTCAGC GGAAGTACCT GGTGGACCCC GGGTTCCTGA GACCCGGGGA GCAGCGCTGG 
TTCGCCCGCT ACCTGGCCAT GCAGACCCTG CAGATCGACG TCTGGGATGG GGACTCCCTG 
CTTCTCATTG GATCTGCTGC CGTTCAGCTG AAGCACCTTC TCCGCCAGGG GCGGCCGGCC 
GTGCAGGTCT CGCATGAGCT GGAGGTTGTG GCGACTGAAT ACGAGCAGGA CGCCATGGTG 
GTGAGTGGAG ACATGACCAA GTTTGGCTGC GTCAGACCCA TCGACGTCCA CACGGTGGTG 
AAGGGCCAGC TGCACTTGAC CTTGGCCAAC GTGGGTCACT TGTGTGAACA GAGAGTAAGG 
AGTCCCAGTT CCCTGCCACC TTCCAGGTCG AGGGTCATCT CAAATGATGG AGTTGGCAAC 
TTCATGGGAG GCAGCCTCCT GACACCCGGG GGCCCCAGAT CAGTGAAAAA TGTGGTCCAA 
GCTCAGAAGC TGGCTGATGT GGACAGCGAA CTGGCCGCCC TGCTCTTCAC ACAACCGAGA 
CCAGGGGCCC GGGGGCCCCA GGGCATCAAT CAAGAGGGGG ATGCCGTCCG CAGGCGCAAG 
CTGGAGCGCA TGAGGGCCGT GCGGCTGCGG GAATGTGGAT TGGAGCCGGG CCGCCGCGGG 
CTGGGCGCGC TGGCCCAGCA GAGCCTCCAT GCCCAGCACG CCCGAGACCT GCAGGTCATT 
GCCGCCTACC GTGAGCGCAC AAAGGCGGAG AGCATCGCCA GCGCACTGAG CACGGCCATC 
ACCACGCAGC ACACCATCCA CGCCACCCTA GGCGCTGCCG AGTTCTTCGA GTTCGCCCTG 
AGGAACCCCC ACAACGCGCA GCACATGGTG ACCATCGAGA TAGACAACCC CGAGCTCAGT 
CTCATCCTGG ATGGTCAGGA GTGGCGGTAC TTCAAGGACA TGGCTGACTT GCACACACCC 
CTGGAGGAGG ACATGTTCCA CCTGCGGGGC AGCCTGGCCC CCCAACTCTT CCTGCGACCT 
CGGGAGACTG CCCACATCCC CTTCAAGTTC CAGACTTTCT CTGTGGGCCC CTGGGCCTCG 
GCACAGGCCT CTGCTGAGCT GAGATCAGAG AAGGACATGG ATGCCGGATC ACCCTATAAG 
TCCAGCATGA TGCCCACCAA ACACGCCAAG GTCCTGTTCC GCGCGGGCAG CGGCAAGCCC 
CTGGCCGTGC TCTGCCTGAC GGTGGAGCTG CAGCCTCACG TGGTGGACCA GGTCTTCCGC 
TTCCACCACC CGGAGCTCAC CTTCCTGAAG AAAGCCATCC GCCTGCCACC CTGGCACACG 
CTTCCAGGTG CCCCTGTAGG GACTTCCAGT GAGGACCCCC CTTTCCATGT TCGATGTAGT 
GACCCGAACA TCATTTGTGA GACACAGAGT GTGGGCCCCG GGGAGCCCCG TGACGTGTTC 
CTGAAGGTGG CCAGTGGCCC TAGCCCAGAG GTCAAGGACT TCTTTGTCAC CATCTACGTA 
GACCGCTGGT TAGCAACACC TGTCCAGACC TGGCAGGTCC ACCTGCACTC GCTGCAGCGA 
GTGGACGTCT CCTGTATCAC TGGCCAGAGG ACCCGCCTGG CTCTCGTCCT CCGGGGGACG 
CAGACAGTTA GGAGAGTGCA GGCTTTCAGC TCACACCCCC AGGAGCTGAC GACGGACCCC 
AGAGGTGTGT TCGTGCTGCC TCCTCACGGG GTGCAGGACT TGCATGTGAG CGTCAGGCCC 
TGCAGGCCGG GCAGCCGCTT CGCGCACCTC CACCTGGTTG ACGTGGACGC CCACCAGCTG 
GTGGCCTCGT GGCTCCTGTG TCTCTCCTGC CGGCCGCCTC TCATCTCCAA GGCCTTCGAG 
ATAAAGCTGC CCGCGGGGAG CGGGAGGGGC GCCAATAAGC GCATCACCTA CACCAATCCC 
TACCCCTCGC GGAGGGCCTA CCGCCTGCAC AGCGACCACC CCCACCTGCT GCAGTTCAAG 
GAGGACGCCT TCCAGGTCGG GGGAGGTGAG ACCTATACCA TCGGCCTGCG GTTTGTGTCC 
CGTCAGAGAG CCGGGGAGGA AGAGATCCTG ATTTACATCA ACGACCACGA AGACAAAAAC 
GAGGAGACCT TTTGCGTGAA GGTCATCTAC CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPHP4 ( XM_024976775.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832543.1 Bos taurus nephrocystin 4 (NPHP4), transcript variant X8, mRNA.

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CloneID	OBa27367
Clone ID Related Accession (Same CDS sequence)	XM_015475180.1 , XM_015466842.1 , XM_015466845.1 , XM_024976773.1 , XM_005217172.4 , XM_015475179.1 , XM_015466844.1 , XM_015466846.1 , XM_005217172.3 , XM_010813390.2 , XM_024976775.1 , XM_024976776.1 , XM_005217171.3 , XM_024976772.1 , XM_010813390.3 , XM_015466843.1 , XM_005217171.4 , XM_015475180.2 , XM_024976774.1
Accession Version	XM_024976775.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4296bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	nephrocystin-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACGACT GGCACAGGAT CTTTGACCAA AACCTGCTGG TCCCTCCGCA CCCACAGAGA 
GCACGCCAGC CTCTGAAGGA ATCGACGGCG TTTCAGTGTG TCCTGAAGTG GCTTGATGGC 
CCGCTAATTA AGCAGGGGGT GCTGGAGCTG CTATCGGAGG TGGGCTGCCA CCTGCGCGTG 
TCTCTCTTCG ATGTCACCTA TCGGCACTTC TTCGGGAGGA CGTGGAGGAG CTCGCCGAGG 
CCTGCAGAGC CGCTCACCAG GCAGCCGGCC AGGATCGTCT TCAATGAGCC CTTGTATTTT 
CACACGTCCT TGAACCACCC TCATATTGTG GCCGTGGTGG AGGTGGTCGT TGAAGGCAGA 
AGACGAGACG GGACCCTCCA GACTCTGTCC TGTGGGTTTG GAATTCTTCG GATCTTCAGC 
CACAAGCCGG AATCTCCAAC TTCCACTTCC CAGGACAAAC GGCTGAGACT GTATCATGGG 
AGCCCGCGAG CCCTCCTCCA CCCGCTCCTT CAGGACCCCA TAGAACAGAA CAAGCACATG 
ACCCTCATTG AGAACTGCAG CCTGCAGTAC ACGCTGAGGC CACACCCGCT CCTGGAGCCC 
GTGTTCCACC TTCTTCCCGA GAACTTGCTG GTGTCCGGTC TGCAGCAGAT ACCTGGCCTC 
CTTCCAGCTC ACGGGGAATC AGGAGATGCC CTCCGGAAGC CCCGCCTGCA GAAGCCTCTG 
ACGTGCTACC TGGATGATCT GTTCTTCACG CTGTATCCCT CGCTGGAGAA GTTCGAGGAG 
GAGCTTCTGG AACTCGTGAT CAGCGATTGC TTCCGGGAGG CGGGCAGCCC GCTGGACGTC 
GGCACCCTGG AGATCCTGGA GCGGCGCCTG TGCGTGGGCG TGCACAATGG GTTGGGCTTC 
GTGCAGCGGC CGCAGGTCCT CGTGCTGGTG CCCGAGCTGG AGGTGGCCTT GACACGCTCT 
GCCAGCTTCA GCCGGAAGGT CGGCTCCTCT TCCAAGGCCA GCTCTGGAAA CCAGGCGCTG 
GTCTTGAGAA GCCGTCTCCG ACTCCCTGAG ATGGTCCGTC ACCCCGCGTT CGCCATTGTC 
TTCCAGCTGG AGTACGTGTT TAACAGCCCC TCGGGGGCAG ACAGCAATGC AGCTTCACTC 
TCCTCCTTGT CCAGCCTGGC ATGTATGCAC ATGGTTCGCT GGGCTGTCTG GAACCCCTCG 
CTGGAAGCCA GCTCCGGGCA GGTGATGTTA CCTCTCCAGG GTGGCATCCA GTCCAACCCT 
TCGCACTGTC TGGTCTATAA GGTACCCCCA GCCAGCATAA GCTCCGAAGA GGTGAAGCAG 
GTGGAGTCAG GGACGGTCCA GTTCCAGTAC TCGCTGAGCC CTGAAGAACA CCCAGGCACA 
CCCACAGCGA ATGCAAGCAG CCCCAAGGCA GAGCGGCGGT CTTTTAGGAA GCCACCCGTG 
TCCCCTTCAA GTCCACCAGC ATCGCCGTCC CAGGGGCTCA CCGCTGCCCA GGACTCACCC 
ATGGGACCAG GGCTGTCACT TTCTCAGCTG GCGGCTTCCC CACCATCCCC AGCTCCCCGC 
CGCCGCTCGG CCAAACCTTC GTCACAGCCA CCTGGGAGCC CTGTGCCCTC CCTGGTCCCA 
GGGGAGTTCC CCTTGGAGGG CAGGATCTCT CACCTGGAGG CTGACCTGAG CCAGACAGCC 
TTAGCCCTGG GAACGTCCGC TGCTGACCAC TTGCAGGAAC TGCCTTTCAC ACCCGTGCAC 
GCCCCGATCA TTGCAGGAAC CCAGACCAGG AGCTCTGGAG GGAAGCTGTC ACGGGCATCC 
CTGGCGCTGC TGCAGTCCGC TGGCTTTCCC GAGATTCTGG ACGCCAACAA GCAACCAGCT 
GAGGCTGTGA GTCCTACAGA CCCCGTGAAG TTCAGCCCTC AGAAGGAAGA ATCAGACTGT 
CTACAAGGCA ATGAGATAGT GTTGCAATTT CTTGCCTTTA GCAGGGTGCC CCAGGATGAG 
CAGGCAGCGC CATGGCCACA GACTGTGTAC TTCACCTTCC AATTCTACCG CTTCCCACCC 
GAGACCACGC CACGGCTGCA GCTCGTGGAG CTGGCCGATG CTGGGAAGAC CAGCTCTGCC 
TCCCTGTCGC ACATCCTCGC CCAGATCCAG AAGGATGGCT CCTTTGATGC GGGGTCTCCG 
GGCTTTCAGC GGAAGTACCT GGTGGACCCC GGGTTCCTGA GACCCGGGGA GCAGCGCTGG 
TTCGCCCGCT ACCTGGCCAT GCAGACCCTG CAGATCGACG TCTGGGATGG GGACTCCCTG 
CTTCTCATTG GATCTGCTGC CGTTCAGCTG AAGCACCTTC TCCGCCAGGG GCGGCCGGCC 
GTGCAGGTCT CGCATGAGCT GGAGGTTGTG GCGACTGAAT ACGAGCAGGA CGCCATGGTG 
GTGAGTGGAG ACATGACCAA GTTTGGCTGC GTCAGACCCA TCGACGTCCA CACGGTGGTG 
AAGGGCCAGC TGCACTTGAC CTTGGCCAAC GTGGGTCACT TGTGTGAACA GAGAGTAAGG 
AGTCCCAGTT CCCTGCCACC TTCCAGGTCG AGGGTCATCT CAAATGATGG AGTTGGCAAC 
TTCATGGGAG GCAGCCTCCT GACACCCGGG GGCCCCAGAT CAGTGAAAAA TGTGGTCCAA 
GCTCAGAAGC TGGCTGATGT GGACAGCGAA CTGGCCGCCC TGCTCTTCAC ACAACCGAGA 
CCAGGGGCCC GGGGGCCCCA GGGCATCAAT CAAGAGGGGG ATGCCGTCCG CAGGCGCAAG 
CTGGAGCGCA TGAGGGCCGT GCGGCTGCGG GAATGTGGAT TGGAGCCGGG CCGCCGCGGG 
CTGGGCGCGC TGGCCCAGCA GAGCCTCCAT GCCCAGCACG CCCGAGACCT GCAGGTCATT 
GCCGCCTACC GTGAGCGCAC AAAGGCGGAG AGCATCGCCA GCGCACTGAG CACGGCCATC 
ACCACGCAGC ACACCATCCA CGCCACCCTA GGCGCTGCCG AGTTCTTCGA GTTCGCCCTG 
AGGAACCCCC ACAACGCGCA GCACATGGTG ACCATCGAGA TAGACAACCC CGAGCTCAGT 
CTCATCCTGG ATGGTCAGGA GTGGCGGTAC TTCAAGGACA TGGCTGACTT GCACACACCC 
CTGGAGGAGG ACATGTTCCA CCTGCGGGGC AGCCTGGCCC CCCAACTCTT CCTGCGACCT 
CGGGAGACTG CCCACATCCC CTTCAAGTTC CAGACTTTCT CTGTGGGCCC CTGGGCCTCG 
GCACAGGCCT CTGCTGAGCT GAGATCAGAG AAGGACATGG ATGCCGGATC ACCCTATAAG 
TCCAGCATGA TGCCCACCAA ACACGCCAAG GTCCTGTTCC GCGCGGGCAG CGGCAAGCCC 
CTGGCCGTGC TCTGCCTGAC GGTGGAGCTG CAGCCTCACG TGGTGGACCA GGTCTTCCGC 
TTCCACCACC CGGAGCTCAC CTTCCTGAAG AAAGCCATCC GCCTGCCACC CTGGCACACG 
CTTCCAGGTG CCCCTGTAGG GACTTCCAGT GAGGACCCCC CTTTCCATGT TCGATGTAGT 
GACCCGAACA TCATTTGTGA GACACAGAGT GTGGGCCCCG GGGAGCCCCG TGACGTGTTC 
CTGAAGGTGG CCAGTGGCCC TAGCCCAGAG GTCAAGGACT TCTTTGTCAC CATCTACGTA 
GACCGCTGGT TAGCAACACC TGTCCAGACC TGGCAGGTCC ACCTGCACTC GCTGCAGCGA 
GTGGACGTCT CCTGTATCAC TGGCCAGAGG ACCCGCCTGG CTCTCGTCCT CCGGGGGACG 
CAGACAGTTA GGAGAGTGCA GGCTTTCAGC TCACACCCCC AGGAGCTGAC GACGGACCCC 
AGAGGTGTGT TCGTGCTGCC TCCTCACGGG GTGCAGGACT TGCATGTGAG CGTCAGGCCC 
TGCAGGCCGG GCAGCCGCTT CGCGCACCTC CACCTGGTTG ACGTGGACGC CCACCAGCTG 
GTGGCCTCGT GGCTCCTGTG TCTCTCCTGC CGGCCGCCTC TCATCTCCAA GGCCTTCGAG 
ATAAAGCTGC CCGCGGGGAG CGGGAGGGGC GCCAATAAGC GCATCACCTA CACCAATCCC 
TACCCCTCGC GGAGGGCCTA CCGCCTGCAC AGCGACCACC CCCACCTGCT GCAGTTCAAG 
GAGGACGCCT TCCAGGTCGG GGGAGGTGAG ACCTATACCA TCGGCCTGCG GTTTGTGTCC 
CGTCAGAGAG CCGGGGAGGA AGAGATCCTG ATTTACATCA ACGACCACGA AGACAAAAAC 
GAGGAGACCT TTTGCGTGAA GGTCATCTAC CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832543.1
CDS	1796..6091
Translation	MHDWHRIFDQNLLVPPHPQRARQPLKESTAFQCVLKWLDGPLIKQGVLELLSEVGCHLRVSLFDVTYRHFFGRTWRSSPRPAEPLTRQPARIVFNEPLYFHTSLNHPHIVAVVEVVVEGRRRDGTLQTLSCGFGILRIFSHKPESPTSTSQDKRLRLYHGSPRALLHPLLQDPIEQNKHMTLIENCSLQYTLRPHPLLEPVFHLLPENLLVSGLQQIPGLLPAHGESGDALRKPRLQKPLTCYLDDLFFTLYPSLEKFEEELLELVISDCFREAGSPLDVGTLEILERRLCVGVHNGLGFVQRPQVLVLVPELEVALTRSASFSRKVGSSSKASSGNQALVLRSRLRLPEMVRHPAFAIVFQLEYVFNSPSGADSNAASLSSLSSLACMHMVRWAVWNPSLEASSGQVMLPLQGGIQSNPSHCLVYKVPPASISSEEVKQVESGTVQFQYSLSPEEHPGTPTANASSPKAERRSFRKPPVSPSSPPASPSQGLTAAQDSPMGPGLSLSQLAASPPSPAPRRRSAKPSSQPPGSPVPSLVPGEFPLEGRISHLEADLSQTALALGTSAADHLQELPFTPVHAPIIAGTQTRSSGGKLSRASLALLQSAGFPEILDANKQPAEAVSPTDPVKFSPQKEESDCLQGNEIVLQFLAFSRVPQDEQAAPWPQTVYFTFQFYRFPPETTPRLQLVELADAGKTSSASLSHILAQIQKDGSFDAGSPGFQRKYLVDPGFLRPGEQRWFARYLAMQTLQIDVWDGDSLLLIGSAAVQLKHLLRQGRPAVQVSHELEVVATEYEQDAMVVSGDMTKFGCVRPIDVHTVVKGQLHLTLANVGHLCEQRVRSPSSLPPSRSRVISNDGVGNFMGGSLLTPGGPRSVKNVVQAQKLADVDSELAALLFTQPRPGARGPQGINQEGDAVRRRKLERMRAVRLRECGLEPGRRGLGALAQQSLHAQHARDLQVIAAYRERTKAESIASALSTAITTQHTIHATLGAAEFFEFALRNPHNAQHMVTIEIDNPELSLILDGQEWRYFKDMADLHTPLEEDMFHLRGSLAPQLFLRPRETAHIPFKFQTFSVGPWASAQASAELRSEKDMDAGSPYKSSMMPTKHAKVLFRAGSGKPLAVLCLTVELQPHVVDQVFRFHHPELTFLKKAIRLPPWHTLPGAPVGTSSEDPPFHVRCSDPNIICETQSVGPGEPRDVFLKVASGPSPEVKDFFVTIYVDRWLATPVQTWQVHLHSLQRVDVSCITGQRTRLALVLRGTQTVRRVQAFSSHPQELTTDPRGVFVLPPHGVQDLHVSVRPCRPGSRFAHLHLVDVDAHQLVASWLLCLSCRPPLISKAFEIKLPAGSGRGANKRITYTNPYPSRRAYRLHSDHPHLLQFKEDAFQVGGGETYTIGLRFVSRQRAGEEEILIYINDHEDKNEETFCVKVIYQ

Target ORF information:

RefSeq Version	XM_024976775.1
Organism	Bos taurus(cattle)
Definition	Bos taurus nephrocystin 4 (NPHP4), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976775.1

ORF Insert Sequence:

ATGCACGACT GGCACAGGAT CTTTGACCAA AACCTGCTGG TCCCTCCGCA CCCACAGAGA 
GCACGCCAGC CTCTGAAGGA ATCGACGGCG TTTCAGTGTG TCCTGAAGTG GCTTGATGGC 
CCGCTAATTA AGCAGGGGGT GCTGGAGCTG CTATCGGAGG TGGGCTGCCA CCTGCGCGTG 
TCTCTCTTCG ATGTCACCTA TCGGCACTTC TTCGGGAGGA CGTGGAGGAG CTCGCCGAGG 
CCTGCAGAGC CGCTCACCAG GCAGCCGGCC AGGATCGTCT TCAATGAGCC CTTGTATTTT 
CACACGTCCT TGAACCACCC TCATATTGTG GCCGTGGTGG AGGTGGTCGT TGAAGGCAGA 
AGACGAGACG GGACCCTCCA GACTCTGTCC TGTGGGTTTG GAATTCTTCG GATCTTCAGC 
CACAAGCCGG AATCTCCAAC TTCCACTTCC CAGGACAAAC GGCTGAGACT GTATCATGGG 
AGCCCGCGAG CCCTCCTCCA CCCGCTCCTT CAGGACCCCA TAGAACAGAA CAAGCACATG 
ACCCTCATTG AGAACTGCAG CCTGCAGTAC ACGCTGAGGC CACACCCGCT CCTGGAGCCC 
GTGTTCCACC TTCTTCCCGA GAACTTGCTG GTGTCCGGTC TGCAGCAGAT ACCTGGCCTC 
CTTCCAGCTC ACGGGGAATC AGGAGATGCC CTCCGGAAGC CCCGCCTGCA GAAGCCTCTG 
ACGTGCTACC TGGATGATCT GTTCTTCACG CTGTATCCCT CGCTGGAGAA GTTCGAGGAG 
GAGCTTCTGG AACTCGTGAT CAGCGATTGC TTCCGGGAGG CGGGCAGCCC GCTGGACGTC 
GGCACCCTGG AGATCCTGGA GCGGCGCCTG TGCGTGGGCG TGCACAATGG GTTGGGCTTC 
GTGCAGCGGC CGCAGGTCCT CGTGCTGGTG CCCGAGCTGG AGGTGGCCTT GACACGCTCT 
GCCAGCTTCA GCCGGAAGGT CGGCTCCTCT TCCAAGGCCA GCTCTGGAAA CCAGGCGCTG 
GTCTTGAGAA GCCGTCTCCG ACTCCCTGAG ATGGTCCGTC ACCCCGCGTT CGCCATTGTC 
TTCCAGCTGG AGTACGTGTT TAACAGCCCC TCGGGGGCAG ACAGCAATGC AGCTTCACTC 
TCCTCCTTGT CCAGCCTGGC ATGTATGCAC ATGGTTCGCT GGGCTGTCTG GAACCCCTCG 
CTGGAAGCCA GCTCCGGGCA GGTGATGTTA CCTCTCCAGG GTGGCATCCA GTCCAACCCT 
TCGCACTGTC TGGTCTATAA GGTACCCCCA GCCAGCATAA GCTCCGAAGA GGTGAAGCAG 
GTGGAGTCAG GGACGGTCCA GTTCCAGTAC TCGCTGAGCC CTGAAGAACA CCCAGGCACA 
CCCACAGCGA ATGCAAGCAG CCCCAAGGCA GAGCGGCGGT CTTTTAGGAA GCCACCCGTG 
TCCCCTTCAA GTCCACCAGC ATCGCCGTCC CAGGGGCTCA CCGCTGCCCA GGACTCACCC 
ATGGGACCAG GGCTGTCACT TTCTCAGCTG GCGGCTTCCC CACCATCCCC AGCTCCCCGC 
CGCCGCTCGG CCAAACCTTC GTCACAGCCA CCTGGGAGCC CTGTGCCCTC CCTGGTCCCA 
GGGGAGTTCC CCTTGGAGGG CAGGATCTCT CACCTGGAGG CTGACCTGAG CCAGACAGCC 
TTAGCCCTGG GAACGTCCGC TGCTGACCAC TTGCAGGAAC TGCCTTTCAC ACCCGTGCAC 
GCCCCGATCA TTGCAGGAAC CCAGACCAGG AGCTCTGGAG GGAAGCTGTC ACGGGCATCC 
CTGGCGCTGC TGCAGTCCGC TGGCTTTCCC GAGATTCTGG ACGCCAACAA GCAACCAGCT 
GAGGCTGTGA GTCCTACAGA CCCCGTGAAG TTCAGCCCTC AGAAGGAAGA ATCAGACTGT 
CTACAAGGCA ATGAGATAGT GTTGCAATTT CTTGCCTTTA GCAGGGTGCC CCAGGATGAG 
CAGGCAGCGC CATGGCCACA GACTGTGTAC TTCACCTTCC AATTCTACCG CTTCCCACCC 
GAGACCACGC CACGGCTGCA GCTCGTGGAG CTGGCCGATG CTGGGAAGAC CAGCTCTGCC 
TCCCTGTCGC ACATCCTCGC CCAGATCCAG AAGGATGGCT CCTTTGATGC GGGGTCTCCG 
GGCTTTCAGC GGAAGTACCT GGTGGACCCC GGGTTCCTGA GACCCGGGGA GCAGCGCTGG 
TTCGCCCGCT ACCTGGCCAT GCAGACCCTG CAGATCGACG TCTGGGATGG GGACTCCCTG 
CTTCTCATTG GATCTGCTGC CGTTCAGCTG AAGCACCTTC TCCGCCAGGG GCGGCCGGCC 
GTGCAGGTCT CGCATGAGCT GGAGGTTGTG GCGACTGAAT ACGAGCAGGA CGCCATGGTG 
GTGAGTGGAG ACATGACCAA GTTTGGCTGC GTCAGACCCA TCGACGTCCA CACGGTGGTG 
AAGGGCCAGC TGCACTTGAC CTTGGCCAAC GTGGGTCACT TGTGTGAACA GAGAGTAAGG 
AGTCCCAGTT CCCTGCCACC TTCCAGGTCG AGGGTCATCT CAAATGATGG AGTTGGCAAC 
TTCATGGGAG GCAGCCTCCT GACACCCGGG GGCCCCAGAT CAGTGAAAAA TGTGGTCCAA 
GCTCAGAAGC TGGCTGATGT GGACAGCGAA CTGGCCGCCC TGCTCTTCAC ACAACCGAGA 
CCAGGGGCCC GGGGGCCCCA GGGCATCAAT CAAGAGGGGG ATGCCGTCCG CAGGCGCAAG 
CTGGAGCGCA TGAGGGCCGT GCGGCTGCGG GAATGTGGAT TGGAGCCGGG CCGCCGCGGG 
CTGGGCGCGC TGGCCCAGCA GAGCCTCCAT GCCCAGCACG CCCGAGACCT GCAGGTCATT 
GCCGCCTACC GTGAGCGCAC AAAGGCGGAG AGCATCGCCA GCGCACTGAG CACGGCCATC 
ACCACGCAGC ACACCATCCA CGCCACCCTA GGCGCTGCCG AGTTCTTCGA GTTCGCCCTG 
AGGAACCCCC ACAACGCGCA GCACATGGTG ACCATCGAGA TAGACAACCC CGAGCTCAGT 
CTCATCCTGG ATGGTCAGGA GTGGCGGTAC TTCAAGGACA TGGCTGACTT GCACACACCC 
CTGGAGGAGG ACATGTTCCA CCTGCGGGGC AGCCTGGCCC CCCAACTCTT CCTGCGACCT 
CGGGAGACTG CCCACATCCC CTTCAAGTTC CAGACTTTCT CTGTGGGCCC CTGGGCCTCG 
GCACAGGCCT CTGCTGAGCT GAGATCAGAG AAGGACATGG ATGCCGGATC ACCCTATAAG 
TCCAGCATGA TGCCCACCAA ACACGCCAAG GTCCTGTTCC GCGCGGGCAG CGGCAAGCCC 
CTGGCCGTGC TCTGCCTGAC GGTGGAGCTG CAGCCTCACG TGGTGGACCA GGTCTTCCGC 
TTCCACCACC CGGAGCTCAC CTTCCTGAAG AAAGCCATCC GCCTGCCACC CTGGCACACG 
CTTCCAGGTG CCCCTGTAGG GACTTCCAGT GAGGACCCCC CTTTCCATGT TCGATGTAGT 
GACCCGAACA TCATTTGTGA GACACAGAGT GTGGGCCCCG GGGAGCCCCG TGACGTGTTC 
CTGAAGGTGG CCAGTGGCCC TAGCCCAGAG GTCAAGGACT TCTTTGTCAC CATCTACGTA 
GACCGCTGGT TAGCAACACC TGTCCAGACC TGGCAGGTCC ACCTGCACTC GCTGCAGCGA 
GTGGACGTCT CCTGTATCAC TGGCCAGAGG ACCCGCCTGG CTCTCGTCCT CCGGGGGACG 
CAGACAGTTA GGAGAGTGCA GGCTTTCAGC TCACACCCCC AGGAGCTGAC GACGGACCCC 
AGAGGTGTGT TCGTGCTGCC TCCTCACGGG GTGCAGGACT TGCATGTGAG CGTCAGGCCC 
TGCAGGCCGG GCAGCCGCTT CGCGCACCTC CACCTGGTTG ACGTGGACGC CCACCAGCTG 
GTGGCCTCGT GGCTCCTGTG TCTCTCCTGC CGGCCGCCTC TCATCTCCAA GGCCTTCGAG 
ATAAAGCTGC CCGCGGGGAG CGGGAGGGGC GCCAATAAGC GCATCACCTA CACCAATCCC 
TACCCCTCGC GGAGGGCCTA CCGCCTGCAC AGCGACCACC CCCACCTGCT GCAGTTCAAG 
GAGGACGCCT TCCAGGTCGG GGGAGGTGAG ACCTATACCA TCGGCCTGCG GTTTGTGTCC 
CGTCAGAGAG CCGGGGAGGA AGAGATCCTG ATTTACATCA ACGACCACGA AGACAAAAAC 
GAGGAGACCT TTTGCGTGAA GGTCATCTAC CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPHP4 ( XM_024976776.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832544.1 Bos taurus nephrocystin 4 (NPHP4), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OBa27367
Clone ID Related Accession (Same CDS sequence)	XM_015475180.1 , XM_015466842.1 , XM_015466845.1 , XM_024976773.1 , XM_005217172.4 , XM_015475179.1 , XM_015466844.1 , XM_015466846.1 , XM_005217172.3 , XM_010813390.2 , XM_024976775.1 , XM_024976776.1 , XM_005217171.3 , XM_024976772.1 , XM_010813390.3 , XM_015466843.1 , XM_005217171.4 , XM_015475180.2 , XM_024976774.1
Accession Version	XM_024976776.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4296bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	nephrocystin-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACGACT GGCACAGGAT CTTTGACCAA AACCTGCTGG TCCCTCCGCA CCCACAGAGA 
GCACGCCAGC CTCTGAAGGA ATCGACGGCG TTTCAGTGTG TCCTGAAGTG GCTTGATGGC 
CCGCTAATTA AGCAGGGGGT GCTGGAGCTG CTATCGGAGG TGGGCTGCCA CCTGCGCGTG 
TCTCTCTTCG ATGTCACCTA TCGGCACTTC TTCGGGAGGA CGTGGAGGAG CTCGCCGAGG 
CCTGCAGAGC CGCTCACCAG GCAGCCGGCC AGGATCGTCT TCAATGAGCC CTTGTATTTT 
CACACGTCCT TGAACCACCC TCATATTGTG GCCGTGGTGG AGGTGGTCGT TGAAGGCAGA 
AGACGAGACG GGACCCTCCA GACTCTGTCC TGTGGGTTTG GAATTCTTCG GATCTTCAGC 
CACAAGCCGG AATCTCCAAC TTCCACTTCC CAGGACAAAC GGCTGAGACT GTATCATGGG 
AGCCCGCGAG CCCTCCTCCA CCCGCTCCTT CAGGACCCCA TAGAACAGAA CAAGCACATG 
ACCCTCATTG AGAACTGCAG CCTGCAGTAC ACGCTGAGGC CACACCCGCT CCTGGAGCCC 
GTGTTCCACC TTCTTCCCGA GAACTTGCTG GTGTCCGGTC TGCAGCAGAT ACCTGGCCTC 
CTTCCAGCTC ACGGGGAATC AGGAGATGCC CTCCGGAAGC CCCGCCTGCA GAAGCCTCTG 
ACGTGCTACC TGGATGATCT GTTCTTCACG CTGTATCCCT CGCTGGAGAA GTTCGAGGAG 
GAGCTTCTGG AACTCGTGAT CAGCGATTGC TTCCGGGAGG CGGGCAGCCC GCTGGACGTC 
GGCACCCTGG AGATCCTGGA GCGGCGCCTG TGCGTGGGCG TGCACAATGG GTTGGGCTTC 
GTGCAGCGGC CGCAGGTCCT CGTGCTGGTG CCCGAGCTGG AGGTGGCCTT GACACGCTCT 
GCCAGCTTCA GCCGGAAGGT CGGCTCCTCT TCCAAGGCCA GCTCTGGAAA CCAGGCGCTG 
GTCTTGAGAA GCCGTCTCCG ACTCCCTGAG ATGGTCCGTC ACCCCGCGTT CGCCATTGTC 
TTCCAGCTGG AGTACGTGTT TAACAGCCCC TCGGGGGCAG ACAGCAATGC AGCTTCACTC 
TCCTCCTTGT CCAGCCTGGC ATGTATGCAC ATGGTTCGCT GGGCTGTCTG GAACCCCTCG 
CTGGAAGCCA GCTCCGGGCA GGTGATGTTA CCTCTCCAGG GTGGCATCCA GTCCAACCCT 
TCGCACTGTC TGGTCTATAA GGTACCCCCA GCCAGCATAA GCTCCGAAGA GGTGAAGCAG 
GTGGAGTCAG GGACGGTCCA GTTCCAGTAC TCGCTGAGCC CTGAAGAACA CCCAGGCACA 
CCCACAGCGA ATGCAAGCAG CCCCAAGGCA GAGCGGCGGT CTTTTAGGAA GCCACCCGTG 
TCCCCTTCAA GTCCACCAGC ATCGCCGTCC CAGGGGCTCA CCGCTGCCCA GGACTCACCC 
ATGGGACCAG GGCTGTCACT TTCTCAGCTG GCGGCTTCCC CACCATCCCC AGCTCCCCGC 
CGCCGCTCGG CCAAACCTTC GTCACAGCCA CCTGGGAGCC CTGTGCCCTC CCTGGTCCCA 
GGGGAGTTCC CCTTGGAGGG CAGGATCTCT CACCTGGAGG CTGACCTGAG CCAGACAGCC 
TTAGCCCTGG GAACGTCCGC TGCTGACCAC TTGCAGGAAC TGCCTTTCAC ACCCGTGCAC 
GCCCCGATCA TTGCAGGAAC CCAGACCAGG AGCTCTGGAG GGAAGCTGTC ACGGGCATCC 
CTGGCGCTGC TGCAGTCCGC TGGCTTTCCC GAGATTCTGG ACGCCAACAA GCAACCAGCT 
GAGGCTGTGA GTCCTACAGA CCCCGTGAAG TTCAGCCCTC AGAAGGAAGA ATCAGACTGT 
CTACAAGGCA ATGAGATAGT GTTGCAATTT CTTGCCTTTA GCAGGGTGCC CCAGGATGAG 
CAGGCAGCGC CATGGCCACA GACTGTGTAC TTCACCTTCC AATTCTACCG CTTCCCACCC 
GAGACCACGC CACGGCTGCA GCTCGTGGAG CTGGCCGATG CTGGGAAGAC CAGCTCTGCC 
TCCCTGTCGC ACATCCTCGC CCAGATCCAG AAGGATGGCT CCTTTGATGC GGGGTCTCCG 
GGCTTTCAGC GGAAGTACCT GGTGGACCCC GGGTTCCTGA GACCCGGGGA GCAGCGCTGG 
TTCGCCCGCT ACCTGGCCAT GCAGACCCTG CAGATCGACG TCTGGGATGG GGACTCCCTG 
CTTCTCATTG GATCTGCTGC CGTTCAGCTG AAGCACCTTC TCCGCCAGGG GCGGCCGGCC 
GTGCAGGTCT CGCATGAGCT GGAGGTTGTG GCGACTGAAT ACGAGCAGGA CGCCATGGTG 
GTGAGTGGAG ACATGACCAA GTTTGGCTGC GTCAGACCCA TCGACGTCCA CACGGTGGTG 
AAGGGCCAGC TGCACTTGAC CTTGGCCAAC GTGGGTCACT TGTGTGAACA GAGAGTAAGG 
AGTCCCAGTT CCCTGCCACC TTCCAGGTCG AGGGTCATCT CAAATGATGG AGTTGGCAAC 
TTCATGGGAG GCAGCCTCCT GACACCCGGG GGCCCCAGAT CAGTGAAAAA TGTGGTCCAA 
GCTCAGAAGC TGGCTGATGT GGACAGCGAA CTGGCCGCCC TGCTCTTCAC ACAACCGAGA 
CCAGGGGCCC GGGGGCCCCA GGGCATCAAT CAAGAGGGGG ATGCCGTCCG CAGGCGCAAG 
CTGGAGCGCA TGAGGGCCGT GCGGCTGCGG GAATGTGGAT TGGAGCCGGG CCGCCGCGGG 
CTGGGCGCGC TGGCCCAGCA GAGCCTCCAT GCCCAGCACG CCCGAGACCT GCAGGTCATT 
GCCGCCTACC GTGAGCGCAC AAAGGCGGAG AGCATCGCCA GCGCACTGAG CACGGCCATC 
ACCACGCAGC ACACCATCCA CGCCACCCTA GGCGCTGCCG AGTTCTTCGA GTTCGCCCTG 
AGGAACCCCC ACAACGCGCA GCACATGGTG ACCATCGAGA TAGACAACCC CGAGCTCAGT 
CTCATCCTGG ATGGTCAGGA GTGGCGGTAC TTCAAGGACA TGGCTGACTT GCACACACCC 
CTGGAGGAGG ACATGTTCCA CCTGCGGGGC AGCCTGGCCC CCCAACTCTT CCTGCGACCT 
CGGGAGACTG CCCACATCCC CTTCAAGTTC CAGACTTTCT CTGTGGGCCC CTGGGCCTCG 
GCACAGGCCT CTGCTGAGCT GAGATCAGAG AAGGACATGG ATGCCGGATC ACCCTATAAG 
TCCAGCATGA TGCCCACCAA ACACGCCAAG GTCCTGTTCC GCGCGGGCAG CGGCAAGCCC 
CTGGCCGTGC TCTGCCTGAC GGTGGAGCTG CAGCCTCACG TGGTGGACCA GGTCTTCCGC 
TTCCACCACC CGGAGCTCAC CTTCCTGAAG AAAGCCATCC GCCTGCCACC CTGGCACACG 
CTTCCAGGTG CCCCTGTAGG GACTTCCAGT GAGGACCCCC CTTTCCATGT TCGATGTAGT 
GACCCGAACA TCATTTGTGA GACACAGAGT GTGGGCCCCG GGGAGCCCCG TGACGTGTTC 
CTGAAGGTGG CCAGTGGCCC TAGCCCAGAG GTCAAGGACT TCTTTGTCAC CATCTACGTA 
GACCGCTGGT TAGCAACACC TGTCCAGACC TGGCAGGTCC ACCTGCACTC GCTGCAGCGA 
GTGGACGTCT CCTGTATCAC TGGCCAGAGG ACCCGCCTGG CTCTCGTCCT CCGGGGGACG 
CAGACAGTTA GGAGAGTGCA GGCTTTCAGC TCACACCCCC AGGAGCTGAC GACGGACCCC 
AGAGGTGTGT TCGTGCTGCC TCCTCACGGG GTGCAGGACT TGCATGTGAG CGTCAGGCCC 
TGCAGGCCGG GCAGCCGCTT CGCGCACCTC CACCTGGTTG ACGTGGACGC CCACCAGCTG 
GTGGCCTCGT GGCTCCTGTG TCTCTCCTGC CGGCCGCCTC TCATCTCCAA GGCCTTCGAG 
ATAAAGCTGC CCGCGGGGAG CGGGAGGGGC GCCAATAAGC GCATCACCTA CACCAATCCC 
TACCCCTCGC GGAGGGCCTA CCGCCTGCAC AGCGACCACC CCCACCTGCT GCAGTTCAAG 
GAGGACGCCT TCCAGGTCGG GGGAGGTGAG ACCTATACCA TCGGCCTGCG GTTTGTGTCC 
CGTCAGAGAG CCGGGGAGGA AGAGATCCTG ATTTACATCA ACGACCACGA AGACAAAAAC 
GAGGAGACCT TTTGCGTGAA GGTCATCTAC CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832544.1
CDS	945..5240
Translation	MHDWHRIFDQNLLVPPHPQRARQPLKESTAFQCVLKWLDGPLIKQGVLELLSEVGCHLRVSLFDVTYRHFFGRTWRSSPRPAEPLTRQPARIVFNEPLYFHTSLNHPHIVAVVEVVVEGRRRDGTLQTLSCGFGILRIFSHKPESPTSTSQDKRLRLYHGSPRALLHPLLQDPIEQNKHMTLIENCSLQYTLRPHPLLEPVFHLLPENLLVSGLQQIPGLLPAHGESGDALRKPRLQKPLTCYLDDLFFTLYPSLEKFEEELLELVISDCFREAGSPLDVGTLEILERRLCVGVHNGLGFVQRPQVLVLVPELEVALTRSASFSRKVGSSSKASSGNQALVLRSRLRLPEMVRHPAFAIVFQLEYVFNSPSGADSNAASLSSLSSLACMHMVRWAVWNPSLEASSGQVMLPLQGGIQSNPSHCLVYKVPPASISSEEVKQVESGTVQFQYSLSPEEHPGTPTANASSPKAERRSFRKPPVSPSSPPASPSQGLTAAQDSPMGPGLSLSQLAASPPSPAPRRRSAKPSSQPPGSPVPSLVPGEFPLEGRISHLEADLSQTALALGTSAADHLQELPFTPVHAPIIAGTQTRSSGGKLSRASLALLQSAGFPEILDANKQPAEAVSPTDPVKFSPQKEESDCLQGNEIVLQFLAFSRVPQDEQAAPWPQTVYFTFQFYRFPPETTPRLQLVELADAGKTSSASLSHILAQIQKDGSFDAGSPGFQRKYLVDPGFLRPGEQRWFARYLAMQTLQIDVWDGDSLLLIGSAAVQLKHLLRQGRPAVQVSHELEVVATEYEQDAMVVSGDMTKFGCVRPIDVHTVVKGQLHLTLANVGHLCEQRVRSPSSLPPSRSRVISNDGVGNFMGGSLLTPGGPRSVKNVVQAQKLADVDSELAALLFTQPRPGARGPQGINQEGDAVRRRKLERMRAVRLRECGLEPGRRGLGALAQQSLHAQHARDLQVIAAYRERTKAESIASALSTAITTQHTIHATLGAAEFFEFALRNPHNAQHMVTIEIDNPELSLILDGQEWRYFKDMADLHTPLEEDMFHLRGSLAPQLFLRPRETAHIPFKFQTFSVGPWASAQASAELRSEKDMDAGSPYKSSMMPTKHAKVLFRAGSGKPLAVLCLTVELQPHVVDQVFRFHHPELTFLKKAIRLPPWHTLPGAPVGTSSEDPPFHVRCSDPNIICETQSVGPGEPRDVFLKVASGPSPEVKDFFVTIYVDRWLATPVQTWQVHLHSLQRVDVSCITGQRTRLALVLRGTQTVRRVQAFSSHPQELTTDPRGVFVLPPHGVQDLHVSVRPCRPGSRFAHLHLVDVDAHQLVASWLLCLSCRPPLISKAFEIKLPAGSGRGANKRITYTNPYPSRRAYRLHSDHPHLLQFKEDAFQVGGGETYTIGLRFVSRQRAGEEEILIYINDHEDKNEETFCVKVIYQ

Target ORF information:

RefSeq Version	XM_024976776.1
Organism	Bos taurus(cattle)
Definition	Bos taurus nephrocystin 4 (NPHP4), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976776.1

ORF Insert Sequence:

ATGCACGACT GGCACAGGAT CTTTGACCAA AACCTGCTGG TCCCTCCGCA CCCACAGAGA 
GCACGCCAGC CTCTGAAGGA ATCGACGGCG TTTCAGTGTG TCCTGAAGTG GCTTGATGGC 
CCGCTAATTA AGCAGGGGGT GCTGGAGCTG CTATCGGAGG TGGGCTGCCA CCTGCGCGTG 
TCTCTCTTCG ATGTCACCTA TCGGCACTTC TTCGGGAGGA CGTGGAGGAG CTCGCCGAGG 
CCTGCAGAGC CGCTCACCAG GCAGCCGGCC AGGATCGTCT TCAATGAGCC CTTGTATTTT 
CACACGTCCT TGAACCACCC TCATATTGTG GCCGTGGTGG AGGTGGTCGT TGAAGGCAGA 
AGACGAGACG GGACCCTCCA GACTCTGTCC TGTGGGTTTG GAATTCTTCG GATCTTCAGC 
CACAAGCCGG AATCTCCAAC TTCCACTTCC CAGGACAAAC GGCTGAGACT GTATCATGGG 
AGCCCGCGAG CCCTCCTCCA CCCGCTCCTT CAGGACCCCA TAGAACAGAA CAAGCACATG 
ACCCTCATTG AGAACTGCAG CCTGCAGTAC ACGCTGAGGC CACACCCGCT CCTGGAGCCC 
GTGTTCCACC TTCTTCCCGA GAACTTGCTG GTGTCCGGTC TGCAGCAGAT ACCTGGCCTC 
CTTCCAGCTC ACGGGGAATC AGGAGATGCC CTCCGGAAGC CCCGCCTGCA GAAGCCTCTG 
ACGTGCTACC TGGATGATCT GTTCTTCACG CTGTATCCCT CGCTGGAGAA GTTCGAGGAG 
GAGCTTCTGG AACTCGTGAT CAGCGATTGC TTCCGGGAGG CGGGCAGCCC GCTGGACGTC 
GGCACCCTGG AGATCCTGGA GCGGCGCCTG TGCGTGGGCG TGCACAATGG GTTGGGCTTC 
GTGCAGCGGC CGCAGGTCCT CGTGCTGGTG CCCGAGCTGG AGGTGGCCTT GACACGCTCT 
GCCAGCTTCA GCCGGAAGGT CGGCTCCTCT TCCAAGGCCA GCTCTGGAAA CCAGGCGCTG 
GTCTTGAGAA GCCGTCTCCG ACTCCCTGAG ATGGTCCGTC ACCCCGCGTT CGCCATTGTC 
TTCCAGCTGG AGTACGTGTT TAACAGCCCC TCGGGGGCAG ACAGCAATGC AGCTTCACTC 
TCCTCCTTGT CCAGCCTGGC ATGTATGCAC ATGGTTCGCT GGGCTGTCTG GAACCCCTCG 
CTGGAAGCCA GCTCCGGGCA GGTGATGTTA CCTCTCCAGG GTGGCATCCA GTCCAACCCT 
TCGCACTGTC TGGTCTATAA GGTACCCCCA GCCAGCATAA GCTCCGAAGA GGTGAAGCAG 
GTGGAGTCAG GGACGGTCCA GTTCCAGTAC TCGCTGAGCC CTGAAGAACA CCCAGGCACA 
CCCACAGCGA ATGCAAGCAG CCCCAAGGCA GAGCGGCGGT CTTTTAGGAA GCCACCCGTG 
TCCCCTTCAA GTCCACCAGC ATCGCCGTCC CAGGGGCTCA CCGCTGCCCA GGACTCACCC 
ATGGGACCAG GGCTGTCACT TTCTCAGCTG GCGGCTTCCC CACCATCCCC AGCTCCCCGC 
CGCCGCTCGG CCAAACCTTC GTCACAGCCA CCTGGGAGCC CTGTGCCCTC CCTGGTCCCA 
GGGGAGTTCC CCTTGGAGGG CAGGATCTCT CACCTGGAGG CTGACCTGAG CCAGACAGCC 
TTAGCCCTGG GAACGTCCGC TGCTGACCAC TTGCAGGAAC TGCCTTTCAC ACCCGTGCAC 
GCCCCGATCA TTGCAGGAAC CCAGACCAGG AGCTCTGGAG GGAAGCTGTC ACGGGCATCC 
CTGGCGCTGC TGCAGTCCGC TGGCTTTCCC GAGATTCTGG ACGCCAACAA GCAACCAGCT 
GAGGCTGTGA GTCCTACAGA CCCCGTGAAG TTCAGCCCTC AGAAGGAAGA ATCAGACTGT 
CTACAAGGCA ATGAGATAGT GTTGCAATTT CTTGCCTTTA GCAGGGTGCC CCAGGATGAG 
CAGGCAGCGC CATGGCCACA GACTGTGTAC TTCACCTTCC AATTCTACCG CTTCCCACCC 
GAGACCACGC CACGGCTGCA GCTCGTGGAG CTGGCCGATG CTGGGAAGAC CAGCTCTGCC 
TCCCTGTCGC ACATCCTCGC CCAGATCCAG AAGGATGGCT CCTTTGATGC GGGGTCTCCG 
GGCTTTCAGC GGAAGTACCT GGTGGACCCC GGGTTCCTGA GACCCGGGGA GCAGCGCTGG 
TTCGCCCGCT ACCTGGCCAT GCAGACCCTG CAGATCGACG TCTGGGATGG GGACTCCCTG 
CTTCTCATTG GATCTGCTGC CGTTCAGCTG AAGCACCTTC TCCGCCAGGG GCGGCCGGCC 
GTGCAGGTCT CGCATGAGCT GGAGGTTGTG GCGACTGAAT ACGAGCAGGA CGCCATGGTG 
GTGAGTGGAG ACATGACCAA GTTTGGCTGC GTCAGACCCA TCGACGTCCA CACGGTGGTG 
AAGGGCCAGC TGCACTTGAC CTTGGCCAAC GTGGGTCACT TGTGTGAACA GAGAGTAAGG 
AGTCCCAGTT CCCTGCCACC TTCCAGGTCG AGGGTCATCT CAAATGATGG AGTTGGCAAC 
TTCATGGGAG GCAGCCTCCT GACACCCGGG GGCCCCAGAT CAGTGAAAAA TGTGGTCCAA 
GCTCAGAAGC TGGCTGATGT GGACAGCGAA CTGGCCGCCC TGCTCTTCAC ACAACCGAGA 
CCAGGGGCCC GGGGGCCCCA GGGCATCAAT CAAGAGGGGG ATGCCGTCCG CAGGCGCAAG 
CTGGAGCGCA TGAGGGCCGT GCGGCTGCGG GAATGTGGAT TGGAGCCGGG CCGCCGCGGG 
CTGGGCGCGC TGGCCCAGCA GAGCCTCCAT GCCCAGCACG CCCGAGACCT GCAGGTCATT 
GCCGCCTACC GTGAGCGCAC AAAGGCGGAG AGCATCGCCA GCGCACTGAG CACGGCCATC 
ACCACGCAGC ACACCATCCA CGCCACCCTA GGCGCTGCCG AGTTCTTCGA GTTCGCCCTG 
AGGAACCCCC ACAACGCGCA GCACATGGTG ACCATCGAGA TAGACAACCC CGAGCTCAGT 
CTCATCCTGG ATGGTCAGGA GTGGCGGTAC TTCAAGGACA TGGCTGACTT GCACACACCC 
CTGGAGGAGG ACATGTTCCA CCTGCGGGGC AGCCTGGCCC CCCAACTCTT CCTGCGACCT 
CGGGAGACTG CCCACATCCC CTTCAAGTTC CAGACTTTCT CTGTGGGCCC CTGGGCCTCG 
GCACAGGCCT CTGCTGAGCT GAGATCAGAG AAGGACATGG ATGCCGGATC ACCCTATAAG 
TCCAGCATGA TGCCCACCAA ACACGCCAAG GTCCTGTTCC GCGCGGGCAG CGGCAAGCCC 
CTGGCCGTGC TCTGCCTGAC GGTGGAGCTG CAGCCTCACG TGGTGGACCA GGTCTTCCGC 
TTCCACCACC CGGAGCTCAC CTTCCTGAAG AAAGCCATCC GCCTGCCACC CTGGCACACG 
CTTCCAGGTG CCCCTGTAGG GACTTCCAGT GAGGACCCCC CTTTCCATGT TCGATGTAGT 
GACCCGAACA TCATTTGTGA GACACAGAGT GTGGGCCCCG GGGAGCCCCG TGACGTGTTC 
CTGAAGGTGG CCAGTGGCCC TAGCCCAGAG GTCAAGGACT TCTTTGTCAC CATCTACGTA 
GACCGCTGGT TAGCAACACC TGTCCAGACC TGGCAGGTCC ACCTGCACTC GCTGCAGCGA 
GTGGACGTCT CCTGTATCAC TGGCCAGAGG ACCCGCCTGG CTCTCGTCCT CCGGGGGACG 
CAGACAGTTA GGAGAGTGCA GGCTTTCAGC TCACACCCCC AGGAGCTGAC GACGGACCCC 
AGAGGTGTGT TCGTGCTGCC TCCTCACGGG GTGCAGGACT TGCATGTGAG CGTCAGGCCC 
TGCAGGCCGG GCAGCCGCTT CGCGCACCTC CACCTGGTTG ACGTGGACGC CCACCAGCTG 
GTGGCCTCGT GGCTCCTGTG TCTCTCCTGC CGGCCGCCTC TCATCTCCAA GGCCTTCGAG 
ATAAAGCTGC CCGCGGGGAG CGGGAGGGGC GCCAATAAGC GCATCACCTA CACCAATCCC 
TACCCCTCGC GGAGGGCCTA CCGCCTGCAC AGCGACCACC CCCACCTGCT GCAGTTCAAG 
GAGGACGCCT TCCAGGTCGG GGGAGGTGAG ACCTATACCA TCGGCCTGCG GTTTGTGTCC 
CGTCAGAGAG CCGGGGAGGA AGAGATCCTG ATTTACATCA ACGACCACGA AGACAAAAAC 
GAGGAGACCT TTTGCGTGAA GGTCATCTAC CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPHP4 ( XM_005217171.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005217228.1 Bos taurus nephronophthisis 4 (NPHP4), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa27367
Clone ID Related Accession (Same CDS sequence)	XM_015475180.1 , XM_015466842.1 , XM_015466845.1 , XM_024976773.1 , XM_005217172.4 , XM_015475179.1 , XM_015466844.1 , XM_015466846.1 , XM_005217172.3 , XM_010813390.2 , XM_024976775.1 , XM_024976776.1 , XM_005217171.3 , XM_024976772.1 , XM_010813390.3 , XM_015466843.1 , XM_005217171.4 , XM_015475180.2 , XM_024976774.1
Accession Version	XM_005217171.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4296bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	nephrocystin-4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104439.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005217171.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACGACT GGCACAGGAT CTTTGACCAA AACCTGCTGG TCCCTCCGCA CCCACAGAGA 
GCACGCCAGC CTCTGAAGGA ATCGACGGCG TTTCAGTGTG TCCTGAAGTG GCTTGATGGC 
CCGCTAATTA AGCAGGGGGT GCTGGAGCTG CTATCGGAGG TGGGCTGCCA CCTGCGCGTG 
TCTCTCTTCG ATGTCACCTA TCGGCACTTC TTCGGGAGGA CGTGGAGGAG CTCGCCGAGG 
CCTGCAGAGC CGCTCACCAG GCAGCCGGCC AGGATCGTCT TCAATGAGCC CTTGTATTTT 
CACACGTCCT TGAACCACCC TCATATTGTG GCCGTGGTGG AGGTGGTCGT TGAAGGCAGA 
AGACGAGACG GGACCCTCCA GACTCTGTCC TGTGGGTTTG GAATTCTTCG GATCTTCAGC 
CACAAGCCGG AATCTCCAAC TTCCACTTCC CAGGACAAAC GGCTGAGACT GTATCATGGG 
AGCCCGCGAG CCCTCCTCCA CCCGCTCCTT CAGGACCCCA TAGAACAGAA CAAGCACATG 
ACCCTCATTG AGAACTGCAG CCTGCAGTAC ACGCTGAGGC CACACCCGCT CCTGGAGCCC 
GTGTTCCACC TTCTTCCCGA GAACTTGCTG GTGTCCGGTC TGCAGCAGAT ACCTGGCCTC 
CTTCCAGCTC ACGGGGAATC AGGAGATGCC CTCCGGAAGC CCCGCCTGCA GAAGCCTCTG 
ACGTGCTACC TGGATGATCT GTTCTTCACG CTGTATCCCT CGCTGGAGAA GTTCGAGGAG 
GAGCTTCTGG AACTCGTGAT CAGCGATTGC TTCCGGGAGG CGGGCAGCCC GCTGGACGTC 
GGCACCCTGG AGATCCTGGA GCGGCGCCTG TGCGTGGGCG TGCACAATGG GTTGGGCTTC 
GTGCAGCGGC CGCAGGTCCT CGTGCTGGTG CCCGAGCTGG AGGTGGCCTT GACACGCTCT 
GCCAGCTTCA GCCGGAAGGT CGGCTCCTCT TCCAAGGCCA GCTCTGGAAA CCAGGCGCTG 
GTCTTGAGAA GCCGTCTCCG ACTCCCTGAG ATGGTCCGTC ACCCCGCGTT CGCCATTGTC 
TTCCAGCTGG AGTACGTGTT TAACAGCCCC TCGGGGGCAG ACAGCAATGC AGCTTCACTC 
TCCTCCTTGT CCAGCCTGGC ATGTATGCAC ATGGTTCGCT GGGCTGTCTG GAACCCCTCG 
CTGGAAGCCA GCTCCGGGCA GGTGATGTTA CCTCTCCAGG GTGGCATCCA GTCCAACCCT 
TCGCACTGTC TGGTCTATAA GGTACCCCCA GCCAGCATAA GCTCCGAAGA GGTGAAGCAG 
GTGGAGTCAG GGACGGTCCA GTTCCAGTAC TCGCTGAGCC CTGAAGAACA CCCAGGCACA 
CCCACAGCGA ATGCAAGCAG CCCCAAGGCA GAGCGGCGGT CTTTTAGGAA GCCACCCGTG 
TCCCCTTCAA GTCCACCAGC ATCGCCGTCC CAGGGGCTCA CCGCTGCCCA GGACTCACCC 
ATGGGACCAG GGCTGTCACT TTCTCAGCTG GCGGCTTCCC CACCATCCCC AGCTCCCCGC 
CGCCGCTCGG CCAAACCTTC GTCACAGCCA CCTGGGAGCC CTGTGCCCTC CCTGGTCCCA 
GGGGAGTTCC CCTTGGAGGG CAGGATCTCT CACCTGGAGG CTGACCTGAG CCAGACAGCC 
TTAGCCCTGG GAACGTCCGC TGCTGACCAC TTGCAGGAAC TGCCTTTCAC ACCCGTGCAC 
GCCCCGATCA TTGCAGGAAC CCAGACCAGG AGCTCTGGAG GGAAGCTGTC ACGGGCATCC 
CTGGCGCTGC TGCAGTCCGC TGGCTTTCCC GAGATTCTGG ACGCCAACAA GCAACCAGCT 
GAGGCTGTGA GTCCTACAGA CCCCGTGAAG TTCAGCCCTC AGAAGGAAGA ATCAGACTGT 
CTACAAGGCA ATGAGATAGT GTTGCAATTT CTTGCCTTTA GCAGGGTGCC CCAGGATGAG 
CAGGCAGCGC CATGGCCACA GACTGTGTAC TTCACCTTCC AATTCTACCG CTTCCCACCC 
GAGACCACGC CACGGCTGCA GCTCGTGGAG CTGGCCGATG CTGGGAAGAC CAGCTCTGCC 
TCCCTGTCGC ACATCCTCGC CCAGATCCAG AAGGATGGCT CCTTTGATGC GGGGTCTCCG 
GGCTTTCAGC GGAAGTACCT GGTGGACCCC GGGTTCCTGA GACCCGGGGA GCAGCGCTGG 
TTCGCCCGCT ACCTGGCCAT GCAGACCCTG CAGATCGACG TCTGGGATGG GGACTCCCTG 
CTTCTCATTG GATCTGCTGC CGTTCAGCTG AAGCACCTTC TCCGCCAGGG GCGGCCGGCC 
GTGCAGGTCT CGCATGAGCT GGAGGTTGTG GCGACTGAAT ACGAGCAGGA CGCCATGGTG 
GTGAGTGGAG ACATGACCAA GTTTGGCTGC GTCAGACCCA TCGACGTCCA CACGGTGGTG 
AAGGGCCAGC TGCACTTGAC CTTGGCCAAC GTGGGTCACT TGTGTGAACA GAGAGTAAGG 
AGTCCCAGTT CCCTGCCACC TTCCAGGTCG AGGGTCATCT CAAATGATGG AGTTGGCAAC 
TTCATGGGAG GCAGCCTCCT GACACCCGGG GGCCCCAGAT CAGTGAAAAA TGTGGTCCAA 
GCTCAGAAGC TGGCTGATGT GGACAGCGAA CTGGCCGCCC TGCTCTTCAC ACAACCGAGA 
CCAGGGGCCC GGGGGCCCCA GGGCATCAAT CAAGAGGGGG ATGCCGTCCG CAGGCGCAAG 
CTGGAGCGCA TGAGGGCCGT GCGGCTGCGG GAATGTGGAT TGGAGCCGGG CCGCCGCGGG 
CTGGGCGCGC TGGCCCAGCA GAGCCTCCAT GCCCAGCACG CCCGAGACCT GCAGGTCATT 
GCCGCCTACC GTGAGCGCAC AAAGGCGGAG AGCATCGCCA GCGCACTGAG CACGGCCATC 
ACCACGCAGC ACACCATCCA CGCCACCCTA GGCGCTGCCG AGTTCTTCGA GTTCGCCCTG 
AGGAACCCCC ACAACGCGCA GCACATGGTG ACCATCGAGA TAGACAACCC CGAGCTCAGT 
CTCATCCTGG ATGGTCAGGA GTGGCGGTAC TTCAAGGACA TGGCTGACTT GCACACACCC 
CTGGAGGAGG ACATGTTCCA CCTGCGGGGC AGCCTGGCCC CCCAACTCTT CCTGCGACCT 
CGGGAGACTG CCCACATCCC CTTCAAGTTC CAGACTTTCT CTGTGGGCCC CTGGGCCTCG 
GCACAGGCCT CTGCTGAGCT GAGATCAGAG AAGGACATGG ATGCCGGATC ACCCTATAAG 
TCCAGCATGA TGCCCACCAA ACACGCCAAG GTCCTGTTCC GCGCGGGCAG CGGCAAGCCC 
CTGGCCGTGC TCTGCCTGAC GGTGGAGCTG CAGCCTCACG TGGTGGACCA GGTCTTCCGC 
TTCCACCACC CGGAGCTCAC CTTCCTGAAG AAAGCCATCC GCCTGCCACC CTGGCACACG 
CTTCCAGGTG CCCCTGTAGG GACTTCCAGT GAGGACCCCC CTTTCCATGT TCGATGTAGT 
GACCCGAACA TCATTTGTGA GACACAGAGT GTGGGCCCCG GGGAGCCCCG TGACGTGTTC 
CTGAAGGTGG CCAGTGGCCC TAGCCCAGAG GTCAAGGACT TCTTTGTCAC CATCTACGTA 
GACCGCTGGT TAGCAACACC TGTCCAGACC TGGCAGGTCC ACCTGCACTC GCTGCAGCGA 
GTGGACGTCT CCTGTATCAC TGGCCAGAGG ACCCGCCTGG CTCTCGTCCT CCGGGGGACG 
CAGACAGTTA GGAGAGTGCA GGCTTTCAGC TCACACCCCC AGGAGCTGAC GACGGACCCC 
AGAGGTGTGT TCGTGCTGCC TCCTCACGGG GTGCAGGACT TGCATGTGAG CGTCAGGCCC 
TGCAGGCCGG GCAGCCGCTT CGCGCACCTC CACCTGGTTG ACGTGGACGC CCACCAGCTG 
GTGGCCTCGT GGCTCCTGTG TCTCTCCTGC CGGCCGCCTC TCATCTCCAA GGCCTTCGAG 
ATAAAGCTGC CCGCGGGGAG CGGGAGGGGC GCCAATAAGC GCATCACCTA CACCAATCCC 
TACCCCTCGC GGAGGGCCTA CCGCCTGCAC AGCGACCACC CCCACCTGCT GCAGTTCAAG 
GAGGACGCCT TCCAGGTCGG GGGAGGTGAG ACCTATACCA TCGGCCTGCG GTTTGTGTCC 
CGTCAGAGAG CCGGGGAGGA AGAGATCCTG ATTTACATCA ACGACCACGA AGACAAAAAC 
GAGGAGACCT TTTGCGTGAA GGTCATCTAC CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005217228.1
CDS	185..4480
Translation	MHDWHRIFDQNLLVPPHPQRARQPLKESTAFQCVLKWLDGPLIKQGVLELLSEVGCHLRVSLFDVTYRHFFGRTWRSSPRPAEPLTRQPARIVFNEPLYFHTSLNHPHIVAVVEVVVEGRRRDGTLQTLSCGFGILRIFSHKPESPTSTSQDKRLRLYHGSPRALLHPLLQDPIEQNKHMTLIENCSLQYTLRPHPLLEPVFHLLPENLLVSGLQQIPGLLPAHGESGDALRKPRLQKPLTCYLDDLFFTLYPSLEKFEEELLELVISDCFREAGSPLDVGTLEILERRLCVGVHNGLGFVQRPQVLVLVPELEVALTRSASFSRKVGSSSKASSGNQALVLRSRLRLPEMVRHPAFAIVFQLEYVFNSPSGADSNAASLSSLSSLACMHMVRWAVWNPSLEASSGQVMLPLQGGIQSNPSHCLVYKVPPASISSEEVKQVESGTVQFQYSLSPEEHPGTPTANASSPKAERRSFRKPPVSPSSPPASPSQGLTAAQDSPMGPGLSLSQLAASPPSPAPRRRSAKPSSQPPGSPVPSLVPGEFPLEGRISHLEADLSQTALALGTSAADHLQELPFTPVHAPIIAGTQTRSSGGKLSRASLALLQSAGFPEILDANKQPAEAVSPTDPVKFSPQKEESDCLQGNEIVLQFLAFSRVPQDEQAAPWPQTVYFTFQFYRFPPETTPRLQLVELADAGKTSSASLSHILAQIQKDGSFDAGSPGFQRKYLVDPGFLRPGEQRWFARYLAMQTLQIDVWDGDSLLLIGSAAVQLKHLLRQGRPAVQVSHELEVVATEYEQDAMVVSGDMTKFGCVRPIDVHTVVKGQLHLTLANVGHLCEQRVRSPSSLPPSRSRVISNDGVGNFMGGSLLTPGGPRSVKNVVQAQKLADVDSELAALLFTQPRPGARGPQGINQEGDAVRRRKLERMRAVRLRECGLEPGRRGLGALAQQSLHAQHARDLQVIAAYRERTKAESIASALSTAITTQHTIHATLGAAEFFEFALRNPHNAQHMVTIEIDNPELSLILDGQEWRYFKDMADLHTPLEEDMFHLRGSLAPQLFLRPRETAHIPFKFQTFSVGPWASAQASAELRSEKDMDAGSPYKSSMMPTKHAKVLFRAGSGKPLAVLCLTVELQPHVVDQVFRFHHPELTFLKKAIRLPPWHTLPGAPVGTSSEDPPFHVRCSDPNIICETQSVGPGEPRDVFLKVASGPSPEVKDFFVTIYVDRWLATPVQTWQVHLHSLQRVDVSCITGQRTRLALVLRGTQTVRRVQAFSSHPQELTTDPRGVFVLPPHGVQDLHVSVRPCRPGSRFAHLHLVDVDAHQLVASWLLCLSCRPPLISKAFEIKLPAGSGRGANKRITYTNPYPSRRAYRLHSDHPHLLQFKEDAFQVGGGETYTIGLRFVSRQRAGEEEILIYINDHEDKNEETFCVKVIYQ

Target ORF information:

RefSeq Version	XM_005217171.3
Organism	Bos taurus(cattle)
Definition	Bos taurus nephronophthisis 4 (NPHP4), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005217171.3

ORF Insert Sequence:

ATGCACGACT GGCACAGGAT CTTTGACCAA AACCTGCTGG TCCCTCCGCA CCCACAGAGA 
GCACGCCAGC CTCTGAAGGA ATCGACGGCG TTTCAGTGTG TCCTGAAGTG GCTTGATGGC 
CCGCTAATTA AGCAGGGGGT GCTGGAGCTG CTATCGGAGG TGGGCTGCCA CCTGCGCGTG 
TCTCTCTTCG ATGTCACCTA TCGGCACTTC TTCGGGAGGA CGTGGAGGAG CTCGCCGAGG 
CCTGCAGAGC CGCTCACCAG GCAGCCGGCC AGGATCGTCT TCAATGAGCC CTTGTATTTT 
CACACGTCCT TGAACCACCC TCATATTGTG GCCGTGGTGG AGGTGGTCGT TGAAGGCAGA 
AGACGAGACG GGACCCTCCA GACTCTGTCC TGTGGGTTTG GAATTCTTCG GATCTTCAGC 
CACAAGCCGG AATCTCCAAC TTCCACTTCC CAGGACAAAC GGCTGAGACT GTATCATGGG 
AGCCCGCGAG CCCTCCTCCA CCCGCTCCTT CAGGACCCCA TAGAACAGAA CAAGCACATG 
ACCCTCATTG AGAACTGCAG CCTGCAGTAC ACGCTGAGGC CACACCCGCT CCTGGAGCCC 
GTGTTCCACC TTCTTCCCGA GAACTTGCTG GTGTCCGGTC TGCAGCAGAT ACCTGGCCTC 
CTTCCAGCTC ACGGGGAATC AGGAGATGCC CTCCGGAAGC CCCGCCTGCA GAAGCCTCTG 
ACGTGCTACC TGGATGATCT GTTCTTCACG CTGTATCCCT CGCTGGAGAA GTTCGAGGAG 
GAGCTTCTGG AACTCGTGAT CAGCGATTGC TTCCGGGAGG CGGGCAGCCC GCTGGACGTC 
GGCACCCTGG AGATCCTGGA GCGGCGCCTG TGCGTGGGCG TGCACAATGG GTTGGGCTTC 
GTGCAGCGGC CGCAGGTCCT CGTGCTGGTG CCCGAGCTGG AGGTGGCCTT GACACGCTCT 
GCCAGCTTCA GCCGGAAGGT CGGCTCCTCT TCCAAGGCCA GCTCTGGAAA CCAGGCGCTG 
GTCTTGAGAA GCCGTCTCCG ACTCCCTGAG ATGGTCCGTC ACCCCGCGTT CGCCATTGTC 
TTCCAGCTGG AGTACGTGTT TAACAGCCCC TCGGGGGCAG ACAGCAATGC AGCTTCACTC 
TCCTCCTTGT CCAGCCTGGC ATGTATGCAC ATGGTTCGCT GGGCTGTCTG GAACCCCTCG 
CTGGAAGCCA GCTCCGGGCA GGTGATGTTA CCTCTCCAGG GTGGCATCCA GTCCAACCCT 
TCGCACTGTC TGGTCTATAA GGTACCCCCA GCCAGCATAA GCTCCGAAGA GGTGAAGCAG 
GTGGAGTCAG GGACGGTCCA GTTCCAGTAC TCGCTGAGCC CTGAAGAACA CCCAGGCACA 
CCCACAGCGA ATGCAAGCAG CCCCAAGGCA GAGCGGCGGT CTTTTAGGAA GCCACCCGTG 
TCCCCTTCAA GTCCACCAGC ATCGCCGTCC CAGGGGCTCA CCGCTGCCCA GGACTCACCC 
ATGGGACCAG GGCTGTCACT TTCTCAGCTG GCGGCTTCCC CACCATCCCC AGCTCCCCGC 
CGCCGCTCGG CCAAACCTTC GTCACAGCCA CCTGGGAGCC CTGTGCCCTC CCTGGTCCCA 
GGGGAGTTCC CCTTGGAGGG CAGGATCTCT CACCTGGAGG CTGACCTGAG CCAGACAGCC 
TTAGCCCTGG GAACGTCCGC TGCTGACCAC TTGCAGGAAC TGCCTTTCAC ACCCGTGCAC 
GCCCCGATCA TTGCAGGAAC CCAGACCAGG AGCTCTGGAG GGAAGCTGTC ACGGGCATCC 
CTGGCGCTGC TGCAGTCCGC TGGCTTTCCC GAGATTCTGG ACGCCAACAA GCAACCAGCT 
GAGGCTGTGA GTCCTACAGA CCCCGTGAAG TTCAGCCCTC AGAAGGAAGA ATCAGACTGT 
CTACAAGGCA ATGAGATAGT GTTGCAATTT CTTGCCTTTA GCAGGGTGCC CCAGGATGAG 
CAGGCAGCGC CATGGCCACA GACTGTGTAC TTCACCTTCC AATTCTACCG CTTCCCACCC 
GAGACCACGC CACGGCTGCA GCTCGTGGAG CTGGCCGATG CTGGGAAGAC CAGCTCTGCC 
TCCCTGTCGC ACATCCTCGC CCAGATCCAG AAGGATGGCT CCTTTGATGC GGGGTCTCCG 
GGCTTTCAGC GGAAGTACCT GGTGGACCCC GGGTTCCTGA GACCCGGGGA GCAGCGCTGG 
TTCGCCCGCT ACCTGGCCAT GCAGACCCTG CAGATCGACG TCTGGGATGG GGACTCCCTG 
CTTCTCATTG GATCTGCTGC CGTTCAGCTG AAGCACCTTC TCCGCCAGGG GCGGCCGGCC 
GTGCAGGTCT CGCATGAGCT GGAGGTTGTG GCGACTGAAT ACGAGCAGGA CGCCATGGTG 
GTGAGTGGAG ACATGACCAA GTTTGGCTGC GTCAGACCCA TCGACGTCCA CACGGTGGTG 
AAGGGCCAGC TGCACTTGAC CTTGGCCAAC GTGGGTCACT TGTGTGAACA GAGAGTAAGG 
AGTCCCAGTT CCCTGCCACC TTCCAGGTCG AGGGTCATCT CAAATGATGG AGTTGGCAAC 
TTCATGGGAG GCAGCCTCCT GACACCCGGG GGCCCCAGAT CAGTGAAAAA TGTGGTCCAA 
GCTCAGAAGC TGGCTGATGT GGACAGCGAA CTGGCCGCCC TGCTCTTCAC ACAACCGAGA 
CCAGGGGCCC GGGGGCCCCA GGGCATCAAT CAAGAGGGGG ATGCCGTCCG CAGGCGCAAG 
CTGGAGCGCA TGAGGGCCGT GCGGCTGCGG GAATGTGGAT TGGAGCCGGG CCGCCGCGGG 
CTGGGCGCGC TGGCCCAGCA GAGCCTCCAT GCCCAGCACG CCCGAGACCT GCAGGTCATT 
GCCGCCTACC GTGAGCGCAC AAAGGCGGAG AGCATCGCCA GCGCACTGAG CACGGCCATC 
ACCACGCAGC ACACCATCCA CGCCACCCTA GGCGCTGCCG AGTTCTTCGA GTTCGCCCTG 
AGGAACCCCC ACAACGCGCA GCACATGGTG ACCATCGAGA TAGACAACCC CGAGCTCAGT 
CTCATCCTGG ATGGTCAGGA GTGGCGGTAC TTCAAGGACA TGGCTGACTT GCACACACCC 
CTGGAGGAGG ACATGTTCCA CCTGCGGGGC AGCCTGGCCC CCCAACTCTT CCTGCGACCT 
CGGGAGACTG CCCACATCCC CTTCAAGTTC CAGACTTTCT CTGTGGGCCC CTGGGCCTCG 
GCACAGGCCT CTGCTGAGCT GAGATCAGAG AAGGACATGG ATGCCGGATC ACCCTATAAG 
TCCAGCATGA TGCCCACCAA ACACGCCAAG GTCCTGTTCC GCGCGGGCAG CGGCAAGCCC 
CTGGCCGTGC TCTGCCTGAC GGTGGAGCTG CAGCCTCACG TGGTGGACCA GGTCTTCCGC 
TTCCACCACC CGGAGCTCAC CTTCCTGAAG AAAGCCATCC GCCTGCCACC CTGGCACACG 
CTTCCAGGTG CCCCTGTAGG GACTTCCAGT GAGGACCCCC CTTTCCATGT TCGATGTAGT 
GACCCGAACA TCATTTGTGA GACACAGAGT GTGGGCCCCG GGGAGCCCCG TGACGTGTTC 
CTGAAGGTGG CCAGTGGCCC TAGCCCAGAG GTCAAGGACT TCTTTGTCAC CATCTACGTA 
GACCGCTGGT TAGCAACACC TGTCCAGACC TGGCAGGTCC ACCTGCACTC GCTGCAGCGA 
GTGGACGTCT CCTGTATCAC TGGCCAGAGG ACCCGCCTGG CTCTCGTCCT CCGGGGGACG 
CAGACAGTTA GGAGAGTGCA GGCTTTCAGC TCACACCCCC AGGAGCTGAC GACGGACCCC 
AGAGGTGTGT TCGTGCTGCC TCCTCACGGG GTGCAGGACT TGCATGTGAG CGTCAGGCCC 
TGCAGGCCGG GCAGCCGCTT CGCGCACCTC CACCTGGTTG ACGTGGACGC CCACCAGCTG 
GTGGCCTCGT GGCTCCTGTG TCTCTCCTGC CGGCCGCCTC TCATCTCCAA GGCCTTCGAG 
ATAAAGCTGC CCGCGGGGAG CGGGAGGGGC GCCAATAAGC GCATCACCTA CACCAATCCC 
TACCCCTCGC GGAGGGCCTA CCGCCTGCAC AGCGACCACC CCCACCTGCT GCAGTTCAAG 
GAGGACGCCT TCCAGGTCGG GGGAGGTGAG ACCTATACCA TCGGCCTGCG GTTTGTGTCC 
CGTCAGAGAG CCGGGGAGGA AGAGATCCTG ATTTACATCA ACGACCACGA AGACAAAAAC 
GAGGAGACCT TTTGCGTGAA GGTCATCTAC CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPHP4 ( XM_024976772.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832540.1 Bos taurus nephrocystin 4 (NPHP4), transcript variant X2, mRNA.

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CloneID	OBa27367
Clone ID Related Accession (Same CDS sequence)	XM_015475180.1 , XM_015466842.1 , XM_015466845.1 , XM_024976773.1 , XM_005217172.4 , XM_015475179.1 , XM_015466844.1 , XM_015466846.1 , XM_005217172.3 , XM_010813390.2 , XM_024976775.1 , XM_024976776.1 , XM_005217171.3 , XM_024976772.1 , XM_010813390.3 , XM_015466843.1 , XM_005217171.4 , XM_015475180.2 , XM_024976774.1
Accession Version	XM_024976772.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4296bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	nephrocystin-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACGACT GGCACAGGAT CTTTGACCAA AACCTGCTGG TCCCTCCGCA CCCACAGAGA 
GCACGCCAGC CTCTGAAGGA ATCGACGGCG TTTCAGTGTG TCCTGAAGTG GCTTGATGGC 
CCGCTAATTA AGCAGGGGGT GCTGGAGCTG CTATCGGAGG TGGGCTGCCA CCTGCGCGTG 
TCTCTCTTCG ATGTCACCTA TCGGCACTTC TTCGGGAGGA CGTGGAGGAG CTCGCCGAGG 
CCTGCAGAGC CGCTCACCAG GCAGCCGGCC AGGATCGTCT TCAATGAGCC CTTGTATTTT 
CACACGTCCT TGAACCACCC TCATATTGTG GCCGTGGTGG AGGTGGTCGT TGAAGGCAGA 
AGACGAGACG GGACCCTCCA GACTCTGTCC TGTGGGTTTG GAATTCTTCG GATCTTCAGC 
CACAAGCCGG AATCTCCAAC TTCCACTTCC CAGGACAAAC GGCTGAGACT GTATCATGGG 
AGCCCGCGAG CCCTCCTCCA CCCGCTCCTT CAGGACCCCA TAGAACAGAA CAAGCACATG 
ACCCTCATTG AGAACTGCAG CCTGCAGTAC ACGCTGAGGC CACACCCGCT CCTGGAGCCC 
GTGTTCCACC TTCTTCCCGA GAACTTGCTG GTGTCCGGTC TGCAGCAGAT ACCTGGCCTC 
CTTCCAGCTC ACGGGGAATC AGGAGATGCC CTCCGGAAGC CCCGCCTGCA GAAGCCTCTG 
ACGTGCTACC TGGATGATCT GTTCTTCACG CTGTATCCCT CGCTGGAGAA GTTCGAGGAG 
GAGCTTCTGG AACTCGTGAT CAGCGATTGC TTCCGGGAGG CGGGCAGCCC GCTGGACGTC 
GGCACCCTGG AGATCCTGGA GCGGCGCCTG TGCGTGGGCG TGCACAATGG GTTGGGCTTC 
GTGCAGCGGC CGCAGGTCCT CGTGCTGGTG CCCGAGCTGG AGGTGGCCTT GACACGCTCT 
GCCAGCTTCA GCCGGAAGGT CGGCTCCTCT TCCAAGGCCA GCTCTGGAAA CCAGGCGCTG 
GTCTTGAGAA GCCGTCTCCG ACTCCCTGAG ATGGTCCGTC ACCCCGCGTT CGCCATTGTC 
TTCCAGCTGG AGTACGTGTT TAACAGCCCC TCGGGGGCAG ACAGCAATGC AGCTTCACTC 
TCCTCCTTGT CCAGCCTGGC ATGTATGCAC ATGGTTCGCT GGGCTGTCTG GAACCCCTCG 
CTGGAAGCCA GCTCCGGGCA GGTGATGTTA CCTCTCCAGG GTGGCATCCA GTCCAACCCT 
TCGCACTGTC TGGTCTATAA GGTACCCCCA GCCAGCATAA GCTCCGAAGA GGTGAAGCAG 
GTGGAGTCAG GGACGGTCCA GTTCCAGTAC TCGCTGAGCC CTGAAGAACA CCCAGGCACA 
CCCACAGCGA ATGCAAGCAG CCCCAAGGCA GAGCGGCGGT CTTTTAGGAA GCCACCCGTG 
TCCCCTTCAA GTCCACCAGC ATCGCCGTCC CAGGGGCTCA CCGCTGCCCA GGACTCACCC 
ATGGGACCAG GGCTGTCACT TTCTCAGCTG GCGGCTTCCC CACCATCCCC AGCTCCCCGC 
CGCCGCTCGG CCAAACCTTC GTCACAGCCA CCTGGGAGCC CTGTGCCCTC CCTGGTCCCA 
GGGGAGTTCC CCTTGGAGGG CAGGATCTCT CACCTGGAGG CTGACCTGAG CCAGACAGCC 
TTAGCCCTGG GAACGTCCGC TGCTGACCAC TTGCAGGAAC TGCCTTTCAC ACCCGTGCAC 
GCCCCGATCA TTGCAGGAAC CCAGACCAGG AGCTCTGGAG GGAAGCTGTC ACGGGCATCC 
CTGGCGCTGC TGCAGTCCGC TGGCTTTCCC GAGATTCTGG ACGCCAACAA GCAACCAGCT 
GAGGCTGTGA GTCCTACAGA CCCCGTGAAG TTCAGCCCTC AGAAGGAAGA ATCAGACTGT 
CTACAAGGCA ATGAGATAGT GTTGCAATTT CTTGCCTTTA GCAGGGTGCC CCAGGATGAG 
CAGGCAGCGC CATGGCCACA GACTGTGTAC TTCACCTTCC AATTCTACCG CTTCCCACCC 
GAGACCACGC CACGGCTGCA GCTCGTGGAG CTGGCCGATG CTGGGAAGAC CAGCTCTGCC 
TCCCTGTCGC ACATCCTCGC CCAGATCCAG AAGGATGGCT CCTTTGATGC GGGGTCTCCG 
GGCTTTCAGC GGAAGTACCT GGTGGACCCC GGGTTCCTGA GACCCGGGGA GCAGCGCTGG 
TTCGCCCGCT ACCTGGCCAT GCAGACCCTG CAGATCGACG TCTGGGATGG GGACTCCCTG 
CTTCTCATTG GATCTGCTGC CGTTCAGCTG AAGCACCTTC TCCGCCAGGG GCGGCCGGCC 
GTGCAGGTCT CGCATGAGCT GGAGGTTGTG GCGACTGAAT ACGAGCAGGA CGCCATGGTG 
GTGAGTGGAG ACATGACCAA GTTTGGCTGC GTCAGACCCA TCGACGTCCA CACGGTGGTG 
AAGGGCCAGC TGCACTTGAC CTTGGCCAAC GTGGGTCACT TGTGTGAACA GAGAGTAAGG 
AGTCCCAGTT CCCTGCCACC TTCCAGGTCG AGGGTCATCT CAAATGATGG AGTTGGCAAC 
TTCATGGGAG GCAGCCTCCT GACACCCGGG GGCCCCAGAT CAGTGAAAAA TGTGGTCCAA 
GCTCAGAAGC TGGCTGATGT GGACAGCGAA CTGGCCGCCC TGCTCTTCAC ACAACCGAGA 
CCAGGGGCCC GGGGGCCCCA GGGCATCAAT CAAGAGGGGG ATGCCGTCCG CAGGCGCAAG 
CTGGAGCGCA TGAGGGCCGT GCGGCTGCGG GAATGTGGAT TGGAGCCGGG CCGCCGCGGG 
CTGGGCGCGC TGGCCCAGCA GAGCCTCCAT GCCCAGCACG CCCGAGACCT GCAGGTCATT 
GCCGCCTACC GTGAGCGCAC AAAGGCGGAG AGCATCGCCA GCGCACTGAG CACGGCCATC 
ACCACGCAGC ACACCATCCA CGCCACCCTA GGCGCTGCCG AGTTCTTCGA GTTCGCCCTG 
AGGAACCCCC ACAACGCGCA GCACATGGTG ACCATCGAGA TAGACAACCC CGAGCTCAGT 
CTCATCCTGG ATGGTCAGGA GTGGCGGTAC TTCAAGGACA TGGCTGACTT GCACACACCC 
CTGGAGGAGG ACATGTTCCA CCTGCGGGGC AGCCTGGCCC CCCAACTCTT CCTGCGACCT 
CGGGAGACTG CCCACATCCC CTTCAAGTTC CAGACTTTCT CTGTGGGCCC CTGGGCCTCG 
GCACAGGCCT CTGCTGAGCT GAGATCAGAG AAGGACATGG ATGCCGGATC ACCCTATAAG 
TCCAGCATGA TGCCCACCAA ACACGCCAAG GTCCTGTTCC GCGCGGGCAG CGGCAAGCCC 
CTGGCCGTGC TCTGCCTGAC GGTGGAGCTG CAGCCTCACG TGGTGGACCA GGTCTTCCGC 
TTCCACCACC CGGAGCTCAC CTTCCTGAAG AAAGCCATCC GCCTGCCACC CTGGCACACG 
CTTCCAGGTG CCCCTGTAGG GACTTCCAGT GAGGACCCCC CTTTCCATGT TCGATGTAGT 
GACCCGAACA TCATTTGTGA GACACAGAGT GTGGGCCCCG GGGAGCCCCG TGACGTGTTC 
CTGAAGGTGG CCAGTGGCCC TAGCCCAGAG GTCAAGGACT TCTTTGTCAC CATCTACGTA 
GACCGCTGGT TAGCAACACC TGTCCAGACC TGGCAGGTCC ACCTGCACTC GCTGCAGCGA 
GTGGACGTCT CCTGTATCAC TGGCCAGAGG ACCCGCCTGG CTCTCGTCCT CCGGGGGACG 
CAGACAGTTA GGAGAGTGCA GGCTTTCAGC TCACACCCCC AGGAGCTGAC GACGGACCCC 
AGAGGTGTGT TCGTGCTGCC TCCTCACGGG GTGCAGGACT TGCATGTGAG CGTCAGGCCC 
TGCAGGCCGG GCAGCCGCTT CGCGCACCTC CACCTGGTTG ACGTGGACGC CCACCAGCTG 
GTGGCCTCGT GGCTCCTGTG TCTCTCCTGC CGGCCGCCTC TCATCTCCAA GGCCTTCGAG 
ATAAAGCTGC CCGCGGGGAG CGGGAGGGGC GCCAATAAGC GCATCACCTA CACCAATCCC 
TACCCCTCGC GGAGGGCCTA CCGCCTGCAC AGCGACCACC CCCACCTGCT GCAGTTCAAG 
GAGGACGCCT TCCAGGTCGG GGGAGGTGAG ACCTATACCA TCGGCCTGCG GTTTGTGTCC 
CGTCAGAGAG CCGGGGAGGA AGAGATCCTG ATTTACATCA ACGACCACGA AGACAAAAAC 
GAGGAGACCT TTTGCGTGAA GGTCATCTAC CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832540.1
CDS	2210..6505
Translation	MHDWHRIFDQNLLVPPHPQRARQPLKESTAFQCVLKWLDGPLIKQGVLELLSEVGCHLRVSLFDVTYRHFFGRTWRSSPRPAEPLTRQPARIVFNEPLYFHTSLNHPHIVAVVEVVVEGRRRDGTLQTLSCGFGILRIFSHKPESPTSTSQDKRLRLYHGSPRALLHPLLQDPIEQNKHMTLIENCSLQYTLRPHPLLEPVFHLLPENLLVSGLQQIPGLLPAHGESGDALRKPRLQKPLTCYLDDLFFTLYPSLEKFEEELLELVISDCFREAGSPLDVGTLEILERRLCVGVHNGLGFVQRPQVLVLVPELEVALTRSASFSRKVGSSSKASSGNQALVLRSRLRLPEMVRHPAFAIVFQLEYVFNSPSGADSNAASLSSLSSLACMHMVRWAVWNPSLEASSGQVMLPLQGGIQSNPSHCLVYKVPPASISSEEVKQVESGTVQFQYSLSPEEHPGTPTANASSPKAERRSFRKPPVSPSSPPASPSQGLTAAQDSPMGPGLSLSQLAASPPSPAPRRRSAKPSSQPPGSPVPSLVPGEFPLEGRISHLEADLSQTALALGTSAADHLQELPFTPVHAPIIAGTQTRSSGGKLSRASLALLQSAGFPEILDANKQPAEAVSPTDPVKFSPQKEESDCLQGNEIVLQFLAFSRVPQDEQAAPWPQTVYFTFQFYRFPPETTPRLQLVELADAGKTSSASLSHILAQIQKDGSFDAGSPGFQRKYLVDPGFLRPGEQRWFARYLAMQTLQIDVWDGDSLLLIGSAAVQLKHLLRQGRPAVQVSHELEVVATEYEQDAMVVSGDMTKFGCVRPIDVHTVVKGQLHLTLANVGHLCEQRVRSPSSLPPSRSRVISNDGVGNFMGGSLLTPGGPRSVKNVVQAQKLADVDSELAALLFTQPRPGARGPQGINQEGDAVRRRKLERMRAVRLRECGLEPGRRGLGALAQQSLHAQHARDLQVIAAYRERTKAESIASALSTAITTQHTIHATLGAAEFFEFALRNPHNAQHMVTIEIDNPELSLILDGQEWRYFKDMADLHTPLEEDMFHLRGSLAPQLFLRPRETAHIPFKFQTFSVGPWASAQASAELRSEKDMDAGSPYKSSMMPTKHAKVLFRAGSGKPLAVLCLTVELQPHVVDQVFRFHHPELTFLKKAIRLPPWHTLPGAPVGTSSEDPPFHVRCSDPNIICETQSVGPGEPRDVFLKVASGPSPEVKDFFVTIYVDRWLATPVQTWQVHLHSLQRVDVSCITGQRTRLALVLRGTQTVRRVQAFSSHPQELTTDPRGVFVLPPHGVQDLHVSVRPCRPGSRFAHLHLVDVDAHQLVASWLLCLSCRPPLISKAFEIKLPAGSGRGANKRITYTNPYPSRRAYRLHSDHPHLLQFKEDAFQVGGGETYTIGLRFVSRQRAGEEEILIYINDHEDKNEETFCVKVIYQ

Target ORF information:

RefSeq Version	XM_024976772.1
Organism	Bos taurus(cattle)
Definition	Bos taurus nephrocystin 4 (NPHP4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976772.1

ORF Insert Sequence:

ATGCACGACT GGCACAGGAT CTTTGACCAA AACCTGCTGG TCCCTCCGCA CCCACAGAGA 
GCACGCCAGC CTCTGAAGGA ATCGACGGCG TTTCAGTGTG TCCTGAAGTG GCTTGATGGC 
CCGCTAATTA AGCAGGGGGT GCTGGAGCTG CTATCGGAGG TGGGCTGCCA CCTGCGCGTG 
TCTCTCTTCG ATGTCACCTA TCGGCACTTC TTCGGGAGGA CGTGGAGGAG CTCGCCGAGG 
CCTGCAGAGC CGCTCACCAG GCAGCCGGCC AGGATCGTCT TCAATGAGCC CTTGTATTTT 
CACACGTCCT TGAACCACCC TCATATTGTG GCCGTGGTGG AGGTGGTCGT TGAAGGCAGA 
AGACGAGACG GGACCCTCCA GACTCTGTCC TGTGGGTTTG GAATTCTTCG GATCTTCAGC 
CACAAGCCGG AATCTCCAAC TTCCACTTCC CAGGACAAAC GGCTGAGACT GTATCATGGG 
AGCCCGCGAG CCCTCCTCCA CCCGCTCCTT CAGGACCCCA TAGAACAGAA CAAGCACATG 
ACCCTCATTG AGAACTGCAG CCTGCAGTAC ACGCTGAGGC CACACCCGCT CCTGGAGCCC 
GTGTTCCACC TTCTTCCCGA GAACTTGCTG GTGTCCGGTC TGCAGCAGAT ACCTGGCCTC 
CTTCCAGCTC ACGGGGAATC AGGAGATGCC CTCCGGAAGC CCCGCCTGCA GAAGCCTCTG 
ACGTGCTACC TGGATGATCT GTTCTTCACG CTGTATCCCT CGCTGGAGAA GTTCGAGGAG 
GAGCTTCTGG AACTCGTGAT CAGCGATTGC TTCCGGGAGG CGGGCAGCCC GCTGGACGTC 
GGCACCCTGG AGATCCTGGA GCGGCGCCTG TGCGTGGGCG TGCACAATGG GTTGGGCTTC 
GTGCAGCGGC CGCAGGTCCT CGTGCTGGTG CCCGAGCTGG AGGTGGCCTT GACACGCTCT 
GCCAGCTTCA GCCGGAAGGT CGGCTCCTCT TCCAAGGCCA GCTCTGGAAA CCAGGCGCTG 
GTCTTGAGAA GCCGTCTCCG ACTCCCTGAG ATGGTCCGTC ACCCCGCGTT CGCCATTGTC 
TTCCAGCTGG AGTACGTGTT TAACAGCCCC TCGGGGGCAG ACAGCAATGC AGCTTCACTC 
TCCTCCTTGT CCAGCCTGGC ATGTATGCAC ATGGTTCGCT GGGCTGTCTG GAACCCCTCG 
CTGGAAGCCA GCTCCGGGCA GGTGATGTTA CCTCTCCAGG GTGGCATCCA GTCCAACCCT 
TCGCACTGTC TGGTCTATAA GGTACCCCCA GCCAGCATAA GCTCCGAAGA GGTGAAGCAG 
GTGGAGTCAG GGACGGTCCA GTTCCAGTAC TCGCTGAGCC CTGAAGAACA CCCAGGCACA 
CCCACAGCGA ATGCAAGCAG CCCCAAGGCA GAGCGGCGGT CTTTTAGGAA GCCACCCGTG 
TCCCCTTCAA GTCCACCAGC ATCGCCGTCC CAGGGGCTCA CCGCTGCCCA GGACTCACCC 
ATGGGACCAG GGCTGTCACT TTCTCAGCTG GCGGCTTCCC CACCATCCCC AGCTCCCCGC 
CGCCGCTCGG CCAAACCTTC GTCACAGCCA CCTGGGAGCC CTGTGCCCTC CCTGGTCCCA 
GGGGAGTTCC CCTTGGAGGG CAGGATCTCT CACCTGGAGG CTGACCTGAG CCAGACAGCC 
TTAGCCCTGG GAACGTCCGC TGCTGACCAC TTGCAGGAAC TGCCTTTCAC ACCCGTGCAC 
GCCCCGATCA TTGCAGGAAC CCAGACCAGG AGCTCTGGAG GGAAGCTGTC ACGGGCATCC 
CTGGCGCTGC TGCAGTCCGC TGGCTTTCCC GAGATTCTGG ACGCCAACAA GCAACCAGCT 
GAGGCTGTGA GTCCTACAGA CCCCGTGAAG TTCAGCCCTC AGAAGGAAGA ATCAGACTGT 
CTACAAGGCA ATGAGATAGT GTTGCAATTT CTTGCCTTTA GCAGGGTGCC CCAGGATGAG 
CAGGCAGCGC CATGGCCACA GACTGTGTAC TTCACCTTCC AATTCTACCG CTTCCCACCC 
GAGACCACGC CACGGCTGCA GCTCGTGGAG CTGGCCGATG CTGGGAAGAC CAGCTCTGCC 
TCCCTGTCGC ACATCCTCGC CCAGATCCAG AAGGATGGCT CCTTTGATGC GGGGTCTCCG 
GGCTTTCAGC GGAAGTACCT GGTGGACCCC GGGTTCCTGA GACCCGGGGA GCAGCGCTGG 
TTCGCCCGCT ACCTGGCCAT GCAGACCCTG CAGATCGACG TCTGGGATGG GGACTCCCTG 
CTTCTCATTG GATCTGCTGC CGTTCAGCTG AAGCACCTTC TCCGCCAGGG GCGGCCGGCC 
GTGCAGGTCT CGCATGAGCT GGAGGTTGTG GCGACTGAAT ACGAGCAGGA CGCCATGGTG 
GTGAGTGGAG ACATGACCAA GTTTGGCTGC GTCAGACCCA TCGACGTCCA CACGGTGGTG 
AAGGGCCAGC TGCACTTGAC CTTGGCCAAC GTGGGTCACT TGTGTGAACA GAGAGTAAGG 
AGTCCCAGTT CCCTGCCACC TTCCAGGTCG AGGGTCATCT CAAATGATGG AGTTGGCAAC 
TTCATGGGAG GCAGCCTCCT GACACCCGGG GGCCCCAGAT CAGTGAAAAA TGTGGTCCAA 
GCTCAGAAGC TGGCTGATGT GGACAGCGAA CTGGCCGCCC TGCTCTTCAC ACAACCGAGA 
CCAGGGGCCC GGGGGCCCCA GGGCATCAAT CAAGAGGGGG ATGCCGTCCG CAGGCGCAAG 
CTGGAGCGCA TGAGGGCCGT GCGGCTGCGG GAATGTGGAT TGGAGCCGGG CCGCCGCGGG 
CTGGGCGCGC TGGCCCAGCA GAGCCTCCAT GCCCAGCACG CCCGAGACCT GCAGGTCATT 
GCCGCCTACC GTGAGCGCAC AAAGGCGGAG AGCATCGCCA GCGCACTGAG CACGGCCATC 
ACCACGCAGC ACACCATCCA CGCCACCCTA GGCGCTGCCG AGTTCTTCGA GTTCGCCCTG 
AGGAACCCCC ACAACGCGCA GCACATGGTG ACCATCGAGA TAGACAACCC CGAGCTCAGT 
CTCATCCTGG ATGGTCAGGA GTGGCGGTAC TTCAAGGACA TGGCTGACTT GCACACACCC 
CTGGAGGAGG ACATGTTCCA CCTGCGGGGC AGCCTGGCCC CCCAACTCTT CCTGCGACCT 
CGGGAGACTG CCCACATCCC CTTCAAGTTC CAGACTTTCT CTGTGGGCCC CTGGGCCTCG 
GCACAGGCCT CTGCTGAGCT GAGATCAGAG AAGGACATGG ATGCCGGATC ACCCTATAAG 
TCCAGCATGA TGCCCACCAA ACACGCCAAG GTCCTGTTCC GCGCGGGCAG CGGCAAGCCC 
CTGGCCGTGC TCTGCCTGAC GGTGGAGCTG CAGCCTCACG TGGTGGACCA GGTCTTCCGC 
TTCCACCACC CGGAGCTCAC CTTCCTGAAG AAAGCCATCC GCCTGCCACC CTGGCACACG 
CTTCCAGGTG CCCCTGTAGG GACTTCCAGT GAGGACCCCC CTTTCCATGT TCGATGTAGT 
GACCCGAACA TCATTTGTGA GACACAGAGT GTGGGCCCCG GGGAGCCCCG TGACGTGTTC 
CTGAAGGTGG CCAGTGGCCC TAGCCCAGAG GTCAAGGACT TCTTTGTCAC CATCTACGTA 
GACCGCTGGT TAGCAACACC TGTCCAGACC TGGCAGGTCC ACCTGCACTC GCTGCAGCGA 
GTGGACGTCT CCTGTATCAC TGGCCAGAGG ACCCGCCTGG CTCTCGTCCT CCGGGGGACG 
CAGACAGTTA GGAGAGTGCA GGCTTTCAGC TCACACCCCC AGGAGCTGAC GACGGACCCC 
AGAGGTGTGT TCGTGCTGCC TCCTCACGGG GTGCAGGACT TGCATGTGAG CGTCAGGCCC 
TGCAGGCCGG GCAGCCGCTT CGCGCACCTC CACCTGGTTG ACGTGGACGC CCACCAGCTG 
GTGGCCTCGT GGCTCCTGTG TCTCTCCTGC CGGCCGCCTC TCATCTCCAA GGCCTTCGAG 
ATAAAGCTGC CCGCGGGGAG CGGGAGGGGC GCCAATAAGC GCATCACCTA CACCAATCCC 
TACCCCTCGC GGAGGGCCTA CCGCCTGCAC AGCGACCACC CCCACCTGCT GCAGTTCAAG 
GAGGACGCCT TCCAGGTCGG GGGAGGTGAG ACCTATACCA TCGGCCTGCG GTTTGTGTCC 
CGTCAGAGAG CCGGGGAGGA AGAGATCCTG ATTTACATCA ACGACCACGA AGACAAAAAC 
GAGGAGACCT TTTGCGTGAA GGTCATCTAC CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPHP4 ( XM_010813390.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010811692.1 Bos taurus nephrocystin 4 (NPHP4), transcript variant X4, mRNA.

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CloneID	OBa27367
Clone ID Related Accession (Same CDS sequence)	XM_015475180.1 , XM_015466842.1 , XM_015466845.1 , XM_024976773.1 , XM_005217172.4 , XM_015475179.1 , XM_015466844.1 , XM_015466846.1 , XM_005217172.3 , XM_010813390.2 , XM_024976775.1 , XM_024976776.1 , XM_005217171.3 , XM_024976772.1 , XM_010813390.3 , XM_015466843.1 , XM_005217171.4 , XM_015475180.2 , XM_024976774.1
Accession Version	XM_010813390.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4296bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	nephrocystin-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010813390.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACGACT GGCACAGGAT CTTTGACCAA AACCTGCTGG TCCCTCCGCA CCCACAGAGA 
GCACGCCAGC CTCTGAAGGA ATCGACGGCG TTTCAGTGTG TCCTGAAGTG GCTTGATGGC 
CCGCTAATTA AGCAGGGGGT GCTGGAGCTG CTATCGGAGG TGGGCTGCCA CCTGCGCGTG 
TCTCTCTTCG ATGTCACCTA TCGGCACTTC TTCGGGAGGA CGTGGAGGAG CTCGCCGAGG 
CCTGCAGAGC CGCTCACCAG GCAGCCGGCC AGGATCGTCT TCAATGAGCC CTTGTATTTT 
CACACGTCCT TGAACCACCC TCATATTGTG GCCGTGGTGG AGGTGGTCGT TGAAGGCAGA 
AGACGAGACG GGACCCTCCA GACTCTGTCC TGTGGGTTTG GAATTCTTCG GATCTTCAGC 
CACAAGCCGG AATCTCCAAC TTCCACTTCC CAGGACAAAC GGCTGAGACT GTATCATGGG 
AGCCCGCGAG CCCTCCTCCA CCCGCTCCTT CAGGACCCCA TAGAACAGAA CAAGCACATG 
ACCCTCATTG AGAACTGCAG CCTGCAGTAC ACGCTGAGGC CACACCCGCT CCTGGAGCCC 
GTGTTCCACC TTCTTCCCGA GAACTTGCTG GTGTCCGGTC TGCAGCAGAT ACCTGGCCTC 
CTTCCAGCTC ACGGGGAATC AGGAGATGCC CTCCGGAAGC CCCGCCTGCA GAAGCCTCTG 
ACGTGCTACC TGGATGATCT GTTCTTCACG CTGTATCCCT CGCTGGAGAA GTTCGAGGAG 
GAGCTTCTGG AACTCGTGAT CAGCGATTGC TTCCGGGAGG CGGGCAGCCC GCTGGACGTC 
GGCACCCTGG AGATCCTGGA GCGGCGCCTG TGCGTGGGCG TGCACAATGG GTTGGGCTTC 
GTGCAGCGGC CGCAGGTCCT CGTGCTGGTG CCCGAGCTGG AGGTGGCCTT GACACGCTCT 
GCCAGCTTCA GCCGGAAGGT CGGCTCCTCT TCCAAGGCCA GCTCTGGAAA CCAGGCGCTG 
GTCTTGAGAA GCCGTCTCCG ACTCCCTGAG ATGGTCCGTC ACCCCGCGTT CGCCATTGTC 
TTCCAGCTGG AGTACGTGTT TAACAGCCCC TCGGGGGCAG ACAGCAATGC AGCTTCACTC 
TCCTCCTTGT CCAGCCTGGC ATGTATGCAC ATGGTTCGCT GGGCTGTCTG GAACCCCTCG 
CTGGAAGCCA GCTCCGGGCA GGTGATGTTA CCTCTCCAGG GTGGCATCCA GTCCAACCCT 
TCGCACTGTC TGGTCTATAA GGTACCCCCA GCCAGCATAA GCTCCGAAGA GGTGAAGCAG 
GTGGAGTCAG GGACGGTCCA GTTCCAGTAC TCGCTGAGCC CTGAAGAACA CCCAGGCACA 
CCCACAGCGA ATGCAAGCAG CCCCAAGGCA GAGCGGCGGT CTTTTAGGAA GCCACCCGTG 
TCCCCTTCAA GTCCACCAGC ATCGCCGTCC CAGGGGCTCA CCGCTGCCCA GGACTCACCC 
ATGGGACCAG GGCTGTCACT TTCTCAGCTG GCGGCTTCCC CACCATCCCC AGCTCCCCGC 
CGCCGCTCGG CCAAACCTTC GTCACAGCCA CCTGGGAGCC CTGTGCCCTC CCTGGTCCCA 
GGGGAGTTCC CCTTGGAGGG CAGGATCTCT CACCTGGAGG CTGACCTGAG CCAGACAGCC 
TTAGCCCTGG GAACGTCCGC TGCTGACCAC TTGCAGGAAC TGCCTTTCAC ACCCGTGCAC 
GCCCCGATCA TTGCAGGAAC CCAGACCAGG AGCTCTGGAG GGAAGCTGTC ACGGGCATCC 
CTGGCGCTGC TGCAGTCCGC TGGCTTTCCC GAGATTCTGG ACGCCAACAA GCAACCAGCT 
GAGGCTGTGA GTCCTACAGA CCCCGTGAAG TTCAGCCCTC AGAAGGAAGA ATCAGACTGT 
CTACAAGGCA ATGAGATAGT GTTGCAATTT CTTGCCTTTA GCAGGGTGCC CCAGGATGAG 
CAGGCAGCGC CATGGCCACA GACTGTGTAC TTCACCTTCC AATTCTACCG CTTCCCACCC 
GAGACCACGC CACGGCTGCA GCTCGTGGAG CTGGCCGATG CTGGGAAGAC CAGCTCTGCC 
TCCCTGTCGC ACATCCTCGC CCAGATCCAG AAGGATGGCT CCTTTGATGC GGGGTCTCCG 
GGCTTTCAGC GGAAGTACCT GGTGGACCCC GGGTTCCTGA GACCCGGGGA GCAGCGCTGG 
TTCGCCCGCT ACCTGGCCAT GCAGACCCTG CAGATCGACG TCTGGGATGG GGACTCCCTG 
CTTCTCATTG GATCTGCTGC CGTTCAGCTG AAGCACCTTC TCCGCCAGGG GCGGCCGGCC 
GTGCAGGTCT CGCATGAGCT GGAGGTTGTG GCGACTGAAT ACGAGCAGGA CGCCATGGTG 
GTGAGTGGAG ACATGACCAA GTTTGGCTGC GTCAGACCCA TCGACGTCCA CACGGTGGTG 
AAGGGCCAGC TGCACTTGAC CTTGGCCAAC GTGGGTCACT TGTGTGAACA GAGAGTAAGG 
AGTCCCAGTT CCCTGCCACC TTCCAGGTCG AGGGTCATCT CAAATGATGG AGTTGGCAAC 
TTCATGGGAG GCAGCCTCCT GACACCCGGG GGCCCCAGAT CAGTGAAAAA TGTGGTCCAA 
GCTCAGAAGC TGGCTGATGT GGACAGCGAA CTGGCCGCCC TGCTCTTCAC ACAACCGAGA 
CCAGGGGCCC GGGGGCCCCA GGGCATCAAT CAAGAGGGGG ATGCCGTCCG CAGGCGCAAG 
CTGGAGCGCA TGAGGGCCGT GCGGCTGCGG GAATGTGGAT TGGAGCCGGG CCGCCGCGGG 
CTGGGCGCGC TGGCCCAGCA GAGCCTCCAT GCCCAGCACG CCCGAGACCT GCAGGTCATT 
GCCGCCTACC GTGAGCGCAC AAAGGCGGAG AGCATCGCCA GCGCACTGAG CACGGCCATC 
ACCACGCAGC ACACCATCCA CGCCACCCTA GGCGCTGCCG AGTTCTTCGA GTTCGCCCTG 
AGGAACCCCC ACAACGCGCA GCACATGGTG ACCATCGAGA TAGACAACCC CGAGCTCAGT 
CTCATCCTGG ATGGTCAGGA GTGGCGGTAC TTCAAGGACA TGGCTGACTT GCACACACCC 
CTGGAGGAGG ACATGTTCCA CCTGCGGGGC AGCCTGGCCC CCCAACTCTT CCTGCGACCT 
CGGGAGACTG CCCACATCCC CTTCAAGTTC CAGACTTTCT CTGTGGGCCC CTGGGCCTCG 
GCACAGGCCT CTGCTGAGCT GAGATCAGAG AAGGACATGG ATGCCGGATC ACCCTATAAG 
TCCAGCATGA TGCCCACCAA ACACGCCAAG GTCCTGTTCC GCGCGGGCAG CGGCAAGCCC 
CTGGCCGTGC TCTGCCTGAC GGTGGAGCTG CAGCCTCACG TGGTGGACCA GGTCTTCCGC 
TTCCACCACC CGGAGCTCAC CTTCCTGAAG AAAGCCATCC GCCTGCCACC CTGGCACACG 
CTTCCAGGTG CCCCTGTAGG GACTTCCAGT GAGGACCCCC CTTTCCATGT TCGATGTAGT 
GACCCGAACA TCATTTGTGA GACACAGAGT GTGGGCCCCG GGGAGCCCCG TGACGTGTTC 
CTGAAGGTGG CCAGTGGCCC TAGCCCAGAG GTCAAGGACT TCTTTGTCAC CATCTACGTA 
GACCGCTGGT TAGCAACACC TGTCCAGACC TGGCAGGTCC ACCTGCACTC GCTGCAGCGA 
GTGGACGTCT CCTGTATCAC TGGCCAGAGG ACCCGCCTGG CTCTCGTCCT CCGGGGGACG 
CAGACAGTTA GGAGAGTGCA GGCTTTCAGC TCACACCCCC AGGAGCTGAC GACGGACCCC 
AGAGGTGTGT TCGTGCTGCC TCCTCACGGG GTGCAGGACT TGCATGTGAG CGTCAGGCCC 
TGCAGGCCGG GCAGCCGCTT CGCGCACCTC CACCTGGTTG ACGTGGACGC CCACCAGCTG 
GTGGCCTCGT GGCTCCTGTG TCTCTCCTGC CGGCCGCCTC TCATCTCCAA GGCCTTCGAG 
ATAAAGCTGC CCGCGGGGAG CGGGAGGGGC GCCAATAAGC GCATCACCTA CACCAATCCC 
TACCCCTCGC GGAGGGCCTA CCGCCTGCAC AGCGACCACC CCCACCTGCT GCAGTTCAAG 
GAGGACGCCT TCCAGGTCGG GGGAGGTGAG ACCTATACCA TCGGCCTGCG GTTTGTGTCC 
CGTCAGAGAG CCGGGGAGGA AGAGATCCTG ATTTACATCA ACGACCACGA AGACAAAAAC 
GAGGAGACCT TTTGCGTGAA GGTCATCTAC CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010811692.1
CDS	818..5113
Translation	MHDWHRIFDQNLLVPPHPQRARQPLKESTAFQCVLKWLDGPLIKQGVLELLSEVGCHLRVSLFDVTYRHFFGRTWRSSPRPAEPLTRQPARIVFNEPLYFHTSLNHPHIVAVVEVVVEGRRRDGTLQTLSCGFGILRIFSHKPESPTSTSQDKRLRLYHGSPRALLHPLLQDPIEQNKHMTLIENCSLQYTLRPHPLLEPVFHLLPENLLVSGLQQIPGLLPAHGESGDALRKPRLQKPLTCYLDDLFFTLYPSLEKFEEELLELVISDCFREAGSPLDVGTLEILERRLCVGVHNGLGFVQRPQVLVLVPELEVALTRSASFSRKVGSSSKASSGNQALVLRSRLRLPEMVRHPAFAIVFQLEYVFNSPSGADSNAASLSSLSSLACMHMVRWAVWNPSLEASSGQVMLPLQGGIQSNPSHCLVYKVPPASISSEEVKQVESGTVQFQYSLSPEEHPGTPTANASSPKAERRSFRKPPVSPSSPPASPSQGLTAAQDSPMGPGLSLSQLAASPPSPAPRRRSAKPSSQPPGSPVPSLVPGEFPLEGRISHLEADLSQTALALGTSAADHLQELPFTPVHAPIIAGTQTRSSGGKLSRASLALLQSAGFPEILDANKQPAEAVSPTDPVKFSPQKEESDCLQGNEIVLQFLAFSRVPQDEQAAPWPQTVYFTFQFYRFPPETTPRLQLVELADAGKTSSASLSHILAQIQKDGSFDAGSPGFQRKYLVDPGFLRPGEQRWFARYLAMQTLQIDVWDGDSLLLIGSAAVQLKHLLRQGRPAVQVSHELEVVATEYEQDAMVVSGDMTKFGCVRPIDVHTVVKGQLHLTLANVGHLCEQRVRSPSSLPPSRSRVISNDGVGNFMGGSLLTPGGPRSVKNVVQAQKLADVDSELAALLFTQPRPGARGPQGINQEGDAVRRRKLERMRAVRLRECGLEPGRRGLGALAQQSLHAQHARDLQVIAAYRERTKAESIASALSTAITTQHTIHATLGAAEFFEFALRNPHNAQHMVTIEIDNPELSLILDGQEWRYFKDMADLHTPLEEDMFHLRGSLAPQLFLRPRETAHIPFKFQTFSVGPWASAQASAELRSEKDMDAGSPYKSSMMPTKHAKVLFRAGSGKPLAVLCLTVELQPHVVDQVFRFHHPELTFLKKAIRLPPWHTLPGAPVGTSSEDPPFHVRCSDPNIICETQSVGPGEPRDVFLKVASGPSPEVKDFFVTIYVDRWLATPVQTWQVHLHSLQRVDVSCITGQRTRLALVLRGTQTVRRVQAFSSHPQELTTDPRGVFVLPPHGVQDLHVSVRPCRPGSRFAHLHLVDVDAHQLVASWLLCLSCRPPLISKAFEIKLPAGSGRGANKRITYTNPYPSRRAYRLHSDHPHLLQFKEDAFQVGGGETYTIGLRFVSRQRAGEEEILIYINDHEDKNEETFCVKVIYQ

Target ORF information:

RefSeq Version	XM_010813390.3
Organism	Bos taurus(cattle)
Definition	Bos taurus nephrocystin 4 (NPHP4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010813390.3

ORF Insert Sequence:

ATGCACGACT GGCACAGGAT CTTTGACCAA AACCTGCTGG TCCCTCCGCA CCCACAGAGA 
GCACGCCAGC CTCTGAAGGA ATCGACGGCG TTTCAGTGTG TCCTGAAGTG GCTTGATGGC 
CCGCTAATTA AGCAGGGGGT GCTGGAGCTG CTATCGGAGG TGGGCTGCCA CCTGCGCGTG 
TCTCTCTTCG ATGTCACCTA TCGGCACTTC TTCGGGAGGA CGTGGAGGAG CTCGCCGAGG 
CCTGCAGAGC CGCTCACCAG GCAGCCGGCC AGGATCGTCT TCAATGAGCC CTTGTATTTT 
CACACGTCCT TGAACCACCC TCATATTGTG GCCGTGGTGG AGGTGGTCGT TGAAGGCAGA 
AGACGAGACG GGACCCTCCA GACTCTGTCC TGTGGGTTTG GAATTCTTCG GATCTTCAGC 
CACAAGCCGG AATCTCCAAC TTCCACTTCC CAGGACAAAC GGCTGAGACT GTATCATGGG 
AGCCCGCGAG CCCTCCTCCA CCCGCTCCTT CAGGACCCCA TAGAACAGAA CAAGCACATG 
ACCCTCATTG AGAACTGCAG CCTGCAGTAC ACGCTGAGGC CACACCCGCT CCTGGAGCCC 
GTGTTCCACC TTCTTCCCGA GAACTTGCTG GTGTCCGGTC TGCAGCAGAT ACCTGGCCTC 
CTTCCAGCTC ACGGGGAATC AGGAGATGCC CTCCGGAAGC CCCGCCTGCA GAAGCCTCTG 
ACGTGCTACC TGGATGATCT GTTCTTCACG CTGTATCCCT CGCTGGAGAA GTTCGAGGAG 
GAGCTTCTGG AACTCGTGAT CAGCGATTGC TTCCGGGAGG CGGGCAGCCC GCTGGACGTC 
GGCACCCTGG AGATCCTGGA GCGGCGCCTG TGCGTGGGCG TGCACAATGG GTTGGGCTTC 
GTGCAGCGGC CGCAGGTCCT CGTGCTGGTG CCCGAGCTGG AGGTGGCCTT GACACGCTCT 
GCCAGCTTCA GCCGGAAGGT CGGCTCCTCT TCCAAGGCCA GCTCTGGAAA CCAGGCGCTG 
GTCTTGAGAA GCCGTCTCCG ACTCCCTGAG ATGGTCCGTC ACCCCGCGTT CGCCATTGTC 
TTCCAGCTGG AGTACGTGTT TAACAGCCCC TCGGGGGCAG ACAGCAATGC AGCTTCACTC 
TCCTCCTTGT CCAGCCTGGC ATGTATGCAC ATGGTTCGCT GGGCTGTCTG GAACCCCTCG 
CTGGAAGCCA GCTCCGGGCA GGTGATGTTA CCTCTCCAGG GTGGCATCCA GTCCAACCCT 
TCGCACTGTC TGGTCTATAA GGTACCCCCA GCCAGCATAA GCTCCGAAGA GGTGAAGCAG 
GTGGAGTCAG GGACGGTCCA GTTCCAGTAC TCGCTGAGCC CTGAAGAACA CCCAGGCACA 
CCCACAGCGA ATGCAAGCAG CCCCAAGGCA GAGCGGCGGT CTTTTAGGAA GCCACCCGTG 
TCCCCTTCAA GTCCACCAGC ATCGCCGTCC CAGGGGCTCA CCGCTGCCCA GGACTCACCC 
ATGGGACCAG GGCTGTCACT TTCTCAGCTG GCGGCTTCCC CACCATCCCC AGCTCCCCGC 
CGCCGCTCGG CCAAACCTTC GTCACAGCCA CCTGGGAGCC CTGTGCCCTC CCTGGTCCCA 
GGGGAGTTCC CCTTGGAGGG CAGGATCTCT CACCTGGAGG CTGACCTGAG CCAGACAGCC 
TTAGCCCTGG GAACGTCCGC TGCTGACCAC TTGCAGGAAC TGCCTTTCAC ACCCGTGCAC 
GCCCCGATCA TTGCAGGAAC CCAGACCAGG AGCTCTGGAG GGAAGCTGTC ACGGGCATCC 
CTGGCGCTGC TGCAGTCCGC TGGCTTTCCC GAGATTCTGG ACGCCAACAA GCAACCAGCT 
GAGGCTGTGA GTCCTACAGA CCCCGTGAAG TTCAGCCCTC AGAAGGAAGA ATCAGACTGT 
CTACAAGGCA ATGAGATAGT GTTGCAATTT CTTGCCTTTA GCAGGGTGCC CCAGGATGAG 
CAGGCAGCGC CATGGCCACA GACTGTGTAC TTCACCTTCC AATTCTACCG CTTCCCACCC 
GAGACCACGC CACGGCTGCA GCTCGTGGAG CTGGCCGATG CTGGGAAGAC CAGCTCTGCC 
TCCCTGTCGC ACATCCTCGC CCAGATCCAG AAGGATGGCT CCTTTGATGC GGGGTCTCCG 
GGCTTTCAGC GGAAGTACCT GGTGGACCCC GGGTTCCTGA GACCCGGGGA GCAGCGCTGG 
TTCGCCCGCT ACCTGGCCAT GCAGACCCTG CAGATCGACG TCTGGGATGG GGACTCCCTG 
CTTCTCATTG GATCTGCTGC CGTTCAGCTG AAGCACCTTC TCCGCCAGGG GCGGCCGGCC 
GTGCAGGTCT CGCATGAGCT GGAGGTTGTG GCGACTGAAT ACGAGCAGGA CGCCATGGTG 
GTGAGTGGAG ACATGACCAA GTTTGGCTGC GTCAGACCCA TCGACGTCCA CACGGTGGTG 
AAGGGCCAGC TGCACTTGAC CTTGGCCAAC GTGGGTCACT TGTGTGAACA GAGAGTAAGG 
AGTCCCAGTT CCCTGCCACC TTCCAGGTCG AGGGTCATCT CAAATGATGG AGTTGGCAAC 
TTCATGGGAG GCAGCCTCCT GACACCCGGG GGCCCCAGAT CAGTGAAAAA TGTGGTCCAA 
GCTCAGAAGC TGGCTGATGT GGACAGCGAA CTGGCCGCCC TGCTCTTCAC ACAACCGAGA 
CCAGGGGCCC GGGGGCCCCA GGGCATCAAT CAAGAGGGGG ATGCCGTCCG CAGGCGCAAG 
CTGGAGCGCA TGAGGGCCGT GCGGCTGCGG GAATGTGGAT TGGAGCCGGG CCGCCGCGGG 
CTGGGCGCGC TGGCCCAGCA GAGCCTCCAT GCCCAGCACG CCCGAGACCT GCAGGTCATT 
GCCGCCTACC GTGAGCGCAC AAAGGCGGAG AGCATCGCCA GCGCACTGAG CACGGCCATC 
ACCACGCAGC ACACCATCCA CGCCACCCTA GGCGCTGCCG AGTTCTTCGA GTTCGCCCTG 
AGGAACCCCC ACAACGCGCA GCACATGGTG ACCATCGAGA TAGACAACCC CGAGCTCAGT 
CTCATCCTGG ATGGTCAGGA GTGGCGGTAC TTCAAGGACA TGGCTGACTT GCACACACCC 
CTGGAGGAGG ACATGTTCCA CCTGCGGGGC AGCCTGGCCC CCCAACTCTT CCTGCGACCT 
CGGGAGACTG CCCACATCCC CTTCAAGTTC CAGACTTTCT CTGTGGGCCC CTGGGCCTCG 
GCACAGGCCT CTGCTGAGCT GAGATCAGAG AAGGACATGG ATGCCGGATC ACCCTATAAG 
TCCAGCATGA TGCCCACCAA ACACGCCAAG GTCCTGTTCC GCGCGGGCAG CGGCAAGCCC 
CTGGCCGTGC TCTGCCTGAC GGTGGAGCTG CAGCCTCACG TGGTGGACCA GGTCTTCCGC 
TTCCACCACC CGGAGCTCAC CTTCCTGAAG AAAGCCATCC GCCTGCCACC CTGGCACACG 
CTTCCAGGTG CCCCTGTAGG GACTTCCAGT GAGGACCCCC CTTTCCATGT TCGATGTAGT 
GACCCGAACA TCATTTGTGA GACACAGAGT GTGGGCCCCG GGGAGCCCCG TGACGTGTTC 
CTGAAGGTGG CCAGTGGCCC TAGCCCAGAG GTCAAGGACT TCTTTGTCAC CATCTACGTA 
GACCGCTGGT TAGCAACACC TGTCCAGACC TGGCAGGTCC ACCTGCACTC GCTGCAGCGA 
GTGGACGTCT CCTGTATCAC TGGCCAGAGG ACCCGCCTGG CTCTCGTCCT CCGGGGGACG 
CAGACAGTTA GGAGAGTGCA GGCTTTCAGC TCACACCCCC AGGAGCTGAC GACGGACCCC 
AGAGGTGTGT TCGTGCTGCC TCCTCACGGG GTGCAGGACT TGCATGTGAG CGTCAGGCCC 
TGCAGGCCGG GCAGCCGCTT CGCGCACCTC CACCTGGTTG ACGTGGACGC CCACCAGCTG 
GTGGCCTCGT GGCTCCTGTG TCTCTCCTGC CGGCCGCCTC TCATCTCCAA GGCCTTCGAG 
ATAAAGCTGC CCGCGGGGAG CGGGAGGGGC GCCAATAAGC GCATCACCTA CACCAATCCC 
TACCCCTCGC GGAGGGCCTA CCGCCTGCAC AGCGACCACC CCCACCTGCT GCAGTTCAAG 
GAGGACGCCT TCCAGGTCGG GGGAGGTGAG ACCTATACCA TCGGCCTGCG GTTTGTGTCC 
CGTCAGAGAG CCGGGGAGGA AGAGATCCTG ATTTACATCA ACGACCACGA AGACAAAAAC 
GAGGAGACCT TTTGCGTGAA GGTCATCTAC CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPHP4 ( XM_015466843.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015322329.1 Bos taurus nephronophthisis 4 (NPHP4), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa27367
Clone ID Related Accession (Same CDS sequence)	XM_015475180.1 , XM_015466842.1 , XM_015466845.1 , XM_024976773.1 , XM_005217172.4 , XM_015475179.1 , XM_015466844.1 , XM_015466846.1 , XM_005217172.3 , XM_010813390.2 , XM_024976775.1 , XM_024976776.1 , XM_005217171.3 , XM_024976772.1 , XM_010813390.3 , XM_015466843.1 , XM_005217171.4 , XM_015475180.2 , XM_024976774.1
Accession Version	XM_015466843.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4296bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	nephrocystin-4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646324.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACGACT GGCACAGGAT CTTTGACCAA AACCTGCTGG TCCCTCCGCA CCCACAGAGA 
GCACGCCAGC CTCTGAAGGA ATCGACGGCG TTTCAGTGTG TCCTGAAGTG GCTTGATGGC 
CCGCTAATTA AGCAGGGGGT GCTGGAGCTG CTATCGGAGG TGGGCTGCCA CCTGCGCGTG 
TCTCTCTTCG ATGTCACCTA TCGGCACTTC TTCGGGAGGA CGTGGAGGAG CTCGCCGAGG 
CCTGCAGAGC CGCTCACCAG GCAGCCGGCC AGGATCGTCT TCAATGAGCC CTTGTATTTT 
CACACGTCCT TGAACCACCC TCATATTGTG GCCGTGGTGG AGGTGGTCGT TGAAGGCAGA 
AGACGAGACG GGACCCTCCA GACTCTGTCC TGTGGGTTTG GAATTCTTCG GATCTTCAGC 
CACAAGCCGG AATCTCCAAC TTCCACTTCC CAGGACAAAC GGCTGAGACT GTATCATGGG 
AGCCCGCGAG CCCTCCTCCA CCCGCTCCTT CAGGACCCCA TAGAACAGAA CAAGCACATG 
ACCCTCATTG AGAACTGCAG CCTGCAGTAC ACGCTGAGGC CACACCCGCT CCTGGAGCCC 
GTGTTCCACC TTCTTCCCGA GAACTTGCTG GTGTCCGGTC TGCAGCAGAT ACCTGGCCTC 
CTTCCAGCTC ACGGGGAATC AGGAGATGCC CTCCGGAAGC CCCGCCTGCA GAAGCCTCTG 
ACGTGCTACC TGGATGATCT GTTCTTCACG CTGTATCCCT CGCTGGAGAA GTTCGAGGAG 
GAGCTTCTGG AACTCGTGAT CAGCGATTGC TTCCGGGAGG CGGGCAGCCC GCTGGACGTC 
GGCACCCTGG AGATCCTGGA GCGGCGCCTG TGCGTGGGCG TGCACAATGG GTTGGGCTTC 
GTGCAGCGGC CGCAGGTCCT CGTGCTGGTG CCCGAGCTGG AGGTGGCCTT GACACGCTCT 
GCCAGCTTCA GCCGGAAGGT CGGCTCCTCT TCCAAGGCCA GCTCTGGAAA CCAGGCGCTG 
GTCTTGAGAA GCCGTCTCCG ACTCCCTGAG ATGGTCCGTC ACCCCGCGTT CGCCATTGTC 
TTCCAGCTGG AGTACGTGTT TAACAGCCCC TCGGGGGCAG ACAGCAATGC AGCTTCACTC 
TCCTCCTTGT CCAGCCTGGC ATGTATGCAC ATGGTTCGCT GGGCTGTCTG GAACCCCTCG 
CTGGAAGCCA GCTCCGGGCA GGTGATGTTA CCTCTCCAGG GTGGCATCCA GTCCAACCCT 
TCGCACTGTC TGGTCTATAA GGTACCCCCA GCCAGCATAA GCTCCGAAGA GGTGAAGCAG 
GTGGAGTCAG GGACGGTCCA GTTCCAGTAC TCGCTGAGCC CTGAAGAACA CCCAGGCACA 
CCCACAGCGA ATGCAAGCAG CCCCAAGGCA GAGCGGCGGT CTTTTAGGAA GCCACCCGTG 
TCCCCTTCAA GTCCACCAGC ATCGCCGTCC CAGGGGCTCA CCGCTGCCCA GGACTCACCC 
ATGGGACCAG GGCTGTCACT TTCTCAGCTG GCGGCTTCCC CACCATCCCC AGCTCCCCGC 
CGCCGCTCGG CCAAACCTTC GTCACAGCCA CCTGGGAGCC CTGTGCCCTC CCTGGTCCCA 
GGGGAGTTCC CCTTGGAGGG CAGGATCTCT CACCTGGAGG CTGACCTGAG CCAGACAGCC 
TTAGCCCTGG GAACGTCCGC TGCTGACCAC TTGCAGGAAC TGCCTTTCAC ACCCGTGCAC 
GCCCCGATCA TTGCAGGAAC CCAGACCAGG AGCTCTGGAG GGAAGCTGTC ACGGGCATCC 
CTGGCGCTGC TGCAGTCCGC TGGCTTTCCC GAGATTCTGG ACGCCAACAA GCAACCAGCT 
GAGGCTGTGA GTCCTACAGA CCCCGTGAAG TTCAGCCCTC AGAAGGAAGA ATCAGACTGT 
CTACAAGGCA ATGAGATAGT GTTGCAATTT CTTGCCTTTA GCAGGGTGCC CCAGGATGAG 
CAGGCAGCGC CATGGCCACA GACTGTGTAC TTCACCTTCC AATTCTACCG CTTCCCACCC 
GAGACCACGC CACGGCTGCA GCTCGTGGAG CTGGCCGATG CTGGGAAGAC CAGCTCTGCC 
TCCCTGTCGC ACATCCTCGC CCAGATCCAG AAGGATGGCT CCTTTGATGC GGGGTCTCCG 
GGCTTTCAGC GGAAGTACCT GGTGGACCCC GGGTTCCTGA GACCCGGGGA GCAGCGCTGG 
TTCGCCCGCT ACCTGGCCAT GCAGACCCTG CAGATCGACG TCTGGGATGG GGACTCCCTG 
CTTCTCATTG GATCTGCTGC CGTTCAGCTG AAGCACCTTC TCCGCCAGGG GCGGCCGGCC 
GTGCAGGTCT CGCATGAGCT GGAGGTTGTG GCGACTGAAT ACGAGCAGGA CGCCATGGTG 
GTGAGTGGAG ACATGACCAA GTTTGGCTGC GTCAGACCCA TCGACGTCCA CACGGTGGTG 
AAGGGCCAGC TGCACTTGAC CTTGGCCAAC GTGGGTCACT TGTGTGAACA GAGAGTAAGG 
AGTCCCAGTT CCCTGCCACC TTCCAGGTCG AGGGTCATCT CAAATGATGG AGTTGGCAAC 
TTCATGGGAG GCAGCCTCCT GACACCCGGG GGCCCCAGAT CAGTGAAAAA TGTGGTCCAA 
GCTCAGAAGC TGGCTGATGT GGACAGCGAA CTGGCCGCCC TGCTCTTCAC ACAACCGAGA 
CCAGGGGCCC GGGGGCCCCA GGGCATCAAT CAAGAGGGGG ATGCCGTCCG CAGGCGCAAG 
CTGGAGCGCA TGAGGGCCGT GCGGCTGCGG GAATGTGGAT TGGAGCCGGG CCGCCGCGGG 
CTGGGCGCGC TGGCCCAGCA GAGCCTCCAT GCCCAGCACG CCCGAGACCT GCAGGTCATT 
GCCGCCTACC GTGAGCGCAC AAAGGCGGAG AGCATCGCCA GCGCACTGAG CACGGCCATC 
ACCACGCAGC ACACCATCCA CGCCACCCTA GGCGCTGCCG AGTTCTTCGA GTTCGCCCTG 
AGGAACCCCC ACAACGCGCA GCACATGGTG ACCATCGAGA TAGACAACCC CGAGCTCAGT 
CTCATCCTGG ATGGTCAGGA GTGGCGGTAC TTCAAGGACA TGGCTGACTT GCACACACCC 
CTGGAGGAGG ACATGTTCCA CCTGCGGGGC AGCCTGGCCC CCCAACTCTT CCTGCGACCT 
CGGGAGACTG CCCACATCCC CTTCAAGTTC CAGACTTTCT CTGTGGGCCC CTGGGCCTCG 
GCACAGGCCT CTGCTGAGCT GAGATCAGAG AAGGACATGG ATGCCGGATC ACCCTATAAG 
TCCAGCATGA TGCCCACCAA ACACGCCAAG GTCCTGTTCC GCGCGGGCAG CGGCAAGCCC 
CTGGCCGTGC TCTGCCTGAC GGTGGAGCTG CAGCCTCACG TGGTGGACCA GGTCTTCCGC 
TTCCACCACC CGGAGCTCAC CTTCCTGAAG AAAGCCATCC GCCTGCCACC CTGGCACACG 
CTTCCAGGTG CCCCTGTAGG GACTTCCAGT GAGGACCCCC CTTTCCATGT TCGATGTAGT 
GACCCGAACA TCATTTGTGA GACACAGAGT GTGGGCCCCG GGGAGCCCCG TGACGTGTTC 
CTGAAGGTGG CCAGTGGCCC TAGCCCAGAG GTCAAGGACT TCTTTGTCAC CATCTACGTA 
GACCGCTGGT TAGCAACACC TGTCCAGACC TGGCAGGTCC ACCTGCACTC GCTGCAGCGA 
GTGGACGTCT CCTGTATCAC TGGCCAGAGG ACCCGCCTGG CTCTCGTCCT CCGGGGGACG 
CAGACAGTTA GGAGAGTGCA GGCTTTCAGC TCACACCCCC AGGAGCTGAC GACGGACCCC 
AGAGGTGTGT TCGTGCTGCC TCCTCACGGG GTGCAGGACT TGCATGTGAG CGTCAGGCCC 
TGCAGGCCGG GCAGCCGCTT CGCGCACCTC CACCTGGTTG ACGTGGACGC CCACCAGCTG 
GTGGCCTCGT GGCTCCTGTG TCTCTCCTGC CGGCCGCCTC TCATCTCCAA GGCCTTCGAG 
ATAAAGCTGC CCGCGGGGAG CGGGAGGGGC GCCAATAAGC GCATCACCTA CACCAATCCC 
TACCCCTCGC GGAGGGCCTA CCGCCTGCAC AGCGACCACC CCCACCTGCT GCAGTTCAAG 
GAGGACGCCT TCCAGGTCGG GGGAGGTGAG ACCTATACCA TCGGCCTGCG GTTTGTGTCC 
CGTCAGAGAG CCGGGGAGGA AGAGATCCTG ATTTACATCA ACGACCACGA AGACAAAAAC 
GAGGAGACCT TTTGCGTGAA GGTCATCTAC CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015322329.1
CDS	1032..5327
Translation	MHDWHRIFDQNLLVPPHPQRARQPLKESTAFQCVLKWLDGPLIKQGVLELLSEVGCHLRVSLFDVTYRHFFGRTWRSSPRPAEPLTRQPARIVFNEPLYFHTSLNHPHIVAVVEVVVEGRRRDGTLQTLSCGFGILRIFSHKPESPTSTSQDKRLRLYHGSPRALLHPLLQDPIEQNKHMTLIENCSLQYTLRPHPLLEPVFHLLPENLLVSGLQQIPGLLPAHGESGDALRKPRLQKPLTCYLDDLFFTLYPSLEKFEEELLELVISDCFREAGSPLDVGTLEILERRLCVGVHNGLGFVQRPQVLVLVPELEVALTRSASFSRKVGSSSKASSGNQALVLRSRLRLPEMVRHPAFAIVFQLEYVFNSPSGADSNAASLSSLSSLACMHMVRWAVWNPSLEASSGQVMLPLQGGIQSNPSHCLVYKVPPASISSEEVKQVESGTVQFQYSLSPEEHPGTPTANASSPKAERRSFRKPPVSPSSPPASPSQGLTAAQDSPMGPGLSLSQLAASPPSPAPRRRSAKPSSQPPGSPVPSLVPGEFPLEGRISHLEADLSQTALALGTSAADHLQELPFTPVHAPIIAGTQTRSSGGKLSRASLALLQSAGFPEILDANKQPAEAVSPTDPVKFSPQKEESDCLQGNEIVLQFLAFSRVPQDEQAAPWPQTVYFTFQFYRFPPETTPRLQLVELADAGKTSSASLSHILAQIQKDGSFDAGSPGFQRKYLVDPGFLRPGEQRWFARYLAMQTLQIDVWDGDSLLLIGSAAVQLKHLLRQGRPAVQVSHELEVVATEYEQDAMVVSGDMTKFGCVRPIDVHTVVKGQLHLTLANVGHLCEQRVRSPSSLPPSRSRVISNDGVGNFMGGSLLTPGGPRSVKNVVQAQKLADVDSELAALLFTQPRPGARGPQGINQEGDAVRRRKLERMRAVRLRECGLEPGRRGLGALAQQSLHAQHARDLQVIAAYRERTKAESIASALSTAITTQHTIHATLGAAEFFEFALRNPHNAQHMVTIEIDNPELSLILDGQEWRYFKDMADLHTPLEEDMFHLRGSLAPQLFLRPRETAHIPFKFQTFSVGPWASAQASAELRSEKDMDAGSPYKSSMMPTKHAKVLFRAGSGKPLAVLCLTVELQPHVVDQVFRFHHPELTFLKKAIRLPPWHTLPGAPVGTSSEDPPFHVRCSDPNIICETQSVGPGEPRDVFLKVASGPSPEVKDFFVTIYVDRWLATPVQTWQVHLHSLQRVDVSCITGQRTRLALVLRGTQTVRRVQAFSSHPQELTTDPRGVFVLPPHGVQDLHVSVRPCRPGSRFAHLHLVDVDAHQLVASWLLCLSCRPPLISKAFEIKLPAGSGRGANKRITYTNPYPSRRAYRLHSDHPHLLQFKEDAFQVGGGETYTIGLRFVSRQRAGEEEILIYINDHEDKNEETFCVKVIYQ

Target ORF information:

RefSeq Version	XM_015466843.1
Organism	Bos taurus(cattle)
Definition	Bos taurus nephronophthisis 4 (NPHP4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015466843.1

ORF Insert Sequence:

ATGCACGACT GGCACAGGAT CTTTGACCAA AACCTGCTGG TCCCTCCGCA CCCACAGAGA 
GCACGCCAGC CTCTGAAGGA ATCGACGGCG TTTCAGTGTG TCCTGAAGTG GCTTGATGGC 
CCGCTAATTA AGCAGGGGGT GCTGGAGCTG CTATCGGAGG TGGGCTGCCA CCTGCGCGTG 
TCTCTCTTCG ATGTCACCTA TCGGCACTTC TTCGGGAGGA CGTGGAGGAG CTCGCCGAGG 
CCTGCAGAGC CGCTCACCAG GCAGCCGGCC AGGATCGTCT TCAATGAGCC CTTGTATTTT 
CACACGTCCT TGAACCACCC TCATATTGTG GCCGTGGTGG AGGTGGTCGT TGAAGGCAGA 
AGACGAGACG GGACCCTCCA GACTCTGTCC TGTGGGTTTG GAATTCTTCG GATCTTCAGC 
CACAAGCCGG AATCTCCAAC TTCCACTTCC CAGGACAAAC GGCTGAGACT GTATCATGGG 
AGCCCGCGAG CCCTCCTCCA CCCGCTCCTT CAGGACCCCA TAGAACAGAA CAAGCACATG 
ACCCTCATTG AGAACTGCAG CCTGCAGTAC ACGCTGAGGC CACACCCGCT CCTGGAGCCC 
GTGTTCCACC TTCTTCCCGA GAACTTGCTG GTGTCCGGTC TGCAGCAGAT ACCTGGCCTC 
CTTCCAGCTC ACGGGGAATC AGGAGATGCC CTCCGGAAGC CCCGCCTGCA GAAGCCTCTG 
ACGTGCTACC TGGATGATCT GTTCTTCACG CTGTATCCCT CGCTGGAGAA GTTCGAGGAG 
GAGCTTCTGG AACTCGTGAT CAGCGATTGC TTCCGGGAGG CGGGCAGCCC GCTGGACGTC 
GGCACCCTGG AGATCCTGGA GCGGCGCCTG TGCGTGGGCG TGCACAATGG GTTGGGCTTC 
GTGCAGCGGC CGCAGGTCCT CGTGCTGGTG CCCGAGCTGG AGGTGGCCTT GACACGCTCT 
GCCAGCTTCA GCCGGAAGGT CGGCTCCTCT TCCAAGGCCA GCTCTGGAAA CCAGGCGCTG 
GTCTTGAGAA GCCGTCTCCG ACTCCCTGAG ATGGTCCGTC ACCCCGCGTT CGCCATTGTC 
TTCCAGCTGG AGTACGTGTT TAACAGCCCC TCGGGGGCAG ACAGCAATGC AGCTTCACTC 
TCCTCCTTGT CCAGCCTGGC ATGTATGCAC ATGGTTCGCT GGGCTGTCTG GAACCCCTCG 
CTGGAAGCCA GCTCCGGGCA GGTGATGTTA CCTCTCCAGG GTGGCATCCA GTCCAACCCT 
TCGCACTGTC TGGTCTATAA GGTACCCCCA GCCAGCATAA GCTCCGAAGA GGTGAAGCAG 
GTGGAGTCAG GGACGGTCCA GTTCCAGTAC TCGCTGAGCC CTGAAGAACA CCCAGGCACA 
CCCACAGCGA ATGCAAGCAG CCCCAAGGCA GAGCGGCGGT CTTTTAGGAA GCCACCCGTG 
TCCCCTTCAA GTCCACCAGC ATCGCCGTCC CAGGGGCTCA CCGCTGCCCA GGACTCACCC 
ATGGGACCAG GGCTGTCACT TTCTCAGCTG GCGGCTTCCC CACCATCCCC AGCTCCCCGC 
CGCCGCTCGG CCAAACCTTC GTCACAGCCA CCTGGGAGCC CTGTGCCCTC CCTGGTCCCA 
GGGGAGTTCC CCTTGGAGGG CAGGATCTCT CACCTGGAGG CTGACCTGAG CCAGACAGCC 
TTAGCCCTGG GAACGTCCGC TGCTGACCAC TTGCAGGAAC TGCCTTTCAC ACCCGTGCAC 
GCCCCGATCA TTGCAGGAAC CCAGACCAGG AGCTCTGGAG GGAAGCTGTC ACGGGCATCC 
CTGGCGCTGC TGCAGTCCGC TGGCTTTCCC GAGATTCTGG ACGCCAACAA GCAACCAGCT 
GAGGCTGTGA GTCCTACAGA CCCCGTGAAG TTCAGCCCTC AGAAGGAAGA ATCAGACTGT 
CTACAAGGCA ATGAGATAGT GTTGCAATTT CTTGCCTTTA GCAGGGTGCC CCAGGATGAG 
CAGGCAGCGC CATGGCCACA GACTGTGTAC TTCACCTTCC AATTCTACCG CTTCCCACCC 
GAGACCACGC CACGGCTGCA GCTCGTGGAG CTGGCCGATG CTGGGAAGAC CAGCTCTGCC 
TCCCTGTCGC ACATCCTCGC CCAGATCCAG AAGGATGGCT CCTTTGATGC GGGGTCTCCG 
GGCTTTCAGC GGAAGTACCT GGTGGACCCC GGGTTCCTGA GACCCGGGGA GCAGCGCTGG 
TTCGCCCGCT ACCTGGCCAT GCAGACCCTG CAGATCGACG TCTGGGATGG GGACTCCCTG 
CTTCTCATTG GATCTGCTGC CGTTCAGCTG AAGCACCTTC TCCGCCAGGG GCGGCCGGCC 
GTGCAGGTCT CGCATGAGCT GGAGGTTGTG GCGACTGAAT ACGAGCAGGA CGCCATGGTG 
GTGAGTGGAG ACATGACCAA GTTTGGCTGC GTCAGACCCA TCGACGTCCA CACGGTGGTG 
AAGGGCCAGC TGCACTTGAC CTTGGCCAAC GTGGGTCACT TGTGTGAACA GAGAGTAAGG 
AGTCCCAGTT CCCTGCCACC TTCCAGGTCG AGGGTCATCT CAAATGATGG AGTTGGCAAC 
TTCATGGGAG GCAGCCTCCT GACACCCGGG GGCCCCAGAT CAGTGAAAAA TGTGGTCCAA 
GCTCAGAAGC TGGCTGATGT GGACAGCGAA CTGGCCGCCC TGCTCTTCAC ACAACCGAGA 
CCAGGGGCCC GGGGGCCCCA GGGCATCAAT CAAGAGGGGG ATGCCGTCCG CAGGCGCAAG 
CTGGAGCGCA TGAGGGCCGT GCGGCTGCGG GAATGTGGAT TGGAGCCGGG CCGCCGCGGG 
CTGGGCGCGC TGGCCCAGCA GAGCCTCCAT GCCCAGCACG CCCGAGACCT GCAGGTCATT 
GCCGCCTACC GTGAGCGCAC AAAGGCGGAG AGCATCGCCA GCGCACTGAG CACGGCCATC 
ACCACGCAGC ACACCATCCA CGCCACCCTA GGCGCTGCCG AGTTCTTCGA GTTCGCCCTG 
AGGAACCCCC ACAACGCGCA GCACATGGTG ACCATCGAGA TAGACAACCC CGAGCTCAGT 
CTCATCCTGG ATGGTCAGGA GTGGCGGTAC TTCAAGGACA TGGCTGACTT GCACACACCC 
CTGGAGGAGG ACATGTTCCA CCTGCGGGGC AGCCTGGCCC CCCAACTCTT CCTGCGACCT 
CGGGAGACTG CCCACATCCC CTTCAAGTTC CAGACTTTCT CTGTGGGCCC CTGGGCCTCG 
GCACAGGCCT CTGCTGAGCT GAGATCAGAG AAGGACATGG ATGCCGGATC ACCCTATAAG 
TCCAGCATGA TGCCCACCAA ACACGCCAAG GTCCTGTTCC GCGCGGGCAG CGGCAAGCCC 
CTGGCCGTGC TCTGCCTGAC GGTGGAGCTG CAGCCTCACG TGGTGGACCA GGTCTTCCGC 
TTCCACCACC CGGAGCTCAC CTTCCTGAAG AAAGCCATCC GCCTGCCACC CTGGCACACG 
CTTCCAGGTG CCCCTGTAGG GACTTCCAGT GAGGACCCCC CTTTCCATGT TCGATGTAGT 
GACCCGAACA TCATTTGTGA GACACAGAGT GTGGGCCCCG GGGAGCCCCG TGACGTGTTC 
CTGAAGGTGG CCAGTGGCCC TAGCCCAGAG GTCAAGGACT TCTTTGTCAC CATCTACGTA 
GACCGCTGGT TAGCAACACC TGTCCAGACC TGGCAGGTCC ACCTGCACTC GCTGCAGCGA 
GTGGACGTCT CCTGTATCAC TGGCCAGAGG ACCCGCCTGG CTCTCGTCCT CCGGGGGACG 
CAGACAGTTA GGAGAGTGCA GGCTTTCAGC TCACACCCCC AGGAGCTGAC GACGGACCCC 
AGAGGTGTGT TCGTGCTGCC TCCTCACGGG GTGCAGGACT TGCATGTGAG CGTCAGGCCC 
TGCAGGCCGG GCAGCCGCTT CGCGCACCTC CACCTGGTTG ACGTGGACGC CCACCAGCTG 
GTGGCCTCGT GGCTCCTGTG TCTCTCCTGC CGGCCGCCTC TCATCTCCAA GGCCTTCGAG 
ATAAAGCTGC CCGCGGGGAG CGGGAGGGGC GCCAATAAGC GCATCACCTA CACCAATCCC 
TACCCCTCGC GGAGGGCCTA CCGCCTGCAC AGCGACCACC CCCACCTGCT GCAGTTCAAG 
GAGGACGCCT TCCAGGTCGG GGGAGGTGAG ACCTATACCA TCGGCCTGCG GTTTGTGTCC 
CGTCAGAGAG CCGGGGAGGA AGAGATCCTG ATTTACATCA ACGACCACGA AGACAAAAAC 
GAGGAGACCT TTTGCGTGAA GGTCATCTAC CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPHP4 ( XM_005217171.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005217228.1 Bos taurus nephrocystin 4 (NPHP4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa27367
Clone ID Related Accession (Same CDS sequence)	XM_015475180.1 , XM_015466842.1 , XM_015466845.1 , XM_024976773.1 , XM_005217172.4 , XM_015475179.1 , XM_015466844.1 , XM_015466846.1 , XM_005217172.3 , XM_010813390.2 , XM_024976775.1 , XM_024976776.1 , XM_005217171.3 , XM_024976772.1 , XM_010813390.3 , XM_015466843.1 , XM_005217171.4 , XM_015475180.2 , XM_024976774.1
Accession Version	XM_005217171.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4296bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	nephrocystin-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005217171.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACGACT GGCACAGGAT CTTTGACCAA AACCTGCTGG TCCCTCCGCA CCCACAGAGA 
GCACGCCAGC CTCTGAAGGA ATCGACGGCG TTTCAGTGTG TCCTGAAGTG GCTTGATGGC 
CCGCTAATTA AGCAGGGGGT GCTGGAGCTG CTATCGGAGG TGGGCTGCCA CCTGCGCGTG 
TCTCTCTTCG ATGTCACCTA TCGGCACTTC TTCGGGAGGA CGTGGAGGAG CTCGCCGAGG 
CCTGCAGAGC CGCTCACCAG GCAGCCGGCC AGGATCGTCT TCAATGAGCC CTTGTATTTT 
CACACGTCCT TGAACCACCC TCATATTGTG GCCGTGGTGG AGGTGGTCGT TGAAGGCAGA 
AGACGAGACG GGACCCTCCA GACTCTGTCC TGTGGGTTTG GAATTCTTCG GATCTTCAGC 
CACAAGCCGG AATCTCCAAC TTCCACTTCC CAGGACAAAC GGCTGAGACT GTATCATGGG 
AGCCCGCGAG CCCTCCTCCA CCCGCTCCTT CAGGACCCCA TAGAACAGAA CAAGCACATG 
ACCCTCATTG AGAACTGCAG CCTGCAGTAC ACGCTGAGGC CACACCCGCT CCTGGAGCCC 
GTGTTCCACC TTCTTCCCGA GAACTTGCTG GTGTCCGGTC TGCAGCAGAT ACCTGGCCTC 
CTTCCAGCTC ACGGGGAATC AGGAGATGCC CTCCGGAAGC CCCGCCTGCA GAAGCCTCTG 
ACGTGCTACC TGGATGATCT GTTCTTCACG CTGTATCCCT CGCTGGAGAA GTTCGAGGAG 
GAGCTTCTGG AACTCGTGAT CAGCGATTGC TTCCGGGAGG CGGGCAGCCC GCTGGACGTC 
GGCACCCTGG AGATCCTGGA GCGGCGCCTG TGCGTGGGCG TGCACAATGG GTTGGGCTTC 
GTGCAGCGGC CGCAGGTCCT CGTGCTGGTG CCCGAGCTGG AGGTGGCCTT GACACGCTCT 
GCCAGCTTCA GCCGGAAGGT CGGCTCCTCT TCCAAGGCCA GCTCTGGAAA CCAGGCGCTG 
GTCTTGAGAA GCCGTCTCCG ACTCCCTGAG ATGGTCCGTC ACCCCGCGTT CGCCATTGTC 
TTCCAGCTGG AGTACGTGTT TAACAGCCCC TCGGGGGCAG ACAGCAATGC AGCTTCACTC 
TCCTCCTTGT CCAGCCTGGC ATGTATGCAC ATGGTTCGCT GGGCTGTCTG GAACCCCTCG 
CTGGAAGCCA GCTCCGGGCA GGTGATGTTA CCTCTCCAGG GTGGCATCCA GTCCAACCCT 
TCGCACTGTC TGGTCTATAA GGTACCCCCA GCCAGCATAA GCTCCGAAGA GGTGAAGCAG 
GTGGAGTCAG GGACGGTCCA GTTCCAGTAC TCGCTGAGCC CTGAAGAACA CCCAGGCACA 
CCCACAGCGA ATGCAAGCAG CCCCAAGGCA GAGCGGCGGT CTTTTAGGAA GCCACCCGTG 
TCCCCTTCAA GTCCACCAGC ATCGCCGTCC CAGGGGCTCA CCGCTGCCCA GGACTCACCC 
ATGGGACCAG GGCTGTCACT TTCTCAGCTG GCGGCTTCCC CACCATCCCC AGCTCCCCGC 
CGCCGCTCGG CCAAACCTTC GTCACAGCCA CCTGGGAGCC CTGTGCCCTC CCTGGTCCCA 
GGGGAGTTCC CCTTGGAGGG CAGGATCTCT CACCTGGAGG CTGACCTGAG CCAGACAGCC 
TTAGCCCTGG GAACGTCCGC TGCTGACCAC TTGCAGGAAC TGCCTTTCAC ACCCGTGCAC 
GCCCCGATCA TTGCAGGAAC CCAGACCAGG AGCTCTGGAG GGAAGCTGTC ACGGGCATCC 
CTGGCGCTGC TGCAGTCCGC TGGCTTTCCC GAGATTCTGG ACGCCAACAA GCAACCAGCT 
GAGGCTGTGA GTCCTACAGA CCCCGTGAAG TTCAGCCCTC AGAAGGAAGA ATCAGACTGT 
CTACAAGGCA ATGAGATAGT GTTGCAATTT CTTGCCTTTA GCAGGGTGCC CCAGGATGAG 
CAGGCAGCGC CATGGCCACA GACTGTGTAC TTCACCTTCC AATTCTACCG CTTCCCACCC 
GAGACCACGC CACGGCTGCA GCTCGTGGAG CTGGCCGATG CTGGGAAGAC CAGCTCTGCC 
TCCCTGTCGC ACATCCTCGC CCAGATCCAG AAGGATGGCT CCTTTGATGC GGGGTCTCCG 
GGCTTTCAGC GGAAGTACCT GGTGGACCCC GGGTTCCTGA GACCCGGGGA GCAGCGCTGG 
TTCGCCCGCT ACCTGGCCAT GCAGACCCTG CAGATCGACG TCTGGGATGG GGACTCCCTG 
CTTCTCATTG GATCTGCTGC CGTTCAGCTG AAGCACCTTC TCCGCCAGGG GCGGCCGGCC 
GTGCAGGTCT CGCATGAGCT GGAGGTTGTG GCGACTGAAT ACGAGCAGGA CGCCATGGTG 
GTGAGTGGAG ACATGACCAA GTTTGGCTGC GTCAGACCCA TCGACGTCCA CACGGTGGTG 
AAGGGCCAGC TGCACTTGAC CTTGGCCAAC GTGGGTCACT TGTGTGAACA GAGAGTAAGG 
AGTCCCAGTT CCCTGCCACC TTCCAGGTCG AGGGTCATCT CAAATGATGG AGTTGGCAAC 
TTCATGGGAG GCAGCCTCCT GACACCCGGG GGCCCCAGAT CAGTGAAAAA TGTGGTCCAA 
GCTCAGAAGC TGGCTGATGT GGACAGCGAA CTGGCCGCCC TGCTCTTCAC ACAACCGAGA 
CCAGGGGCCC GGGGGCCCCA GGGCATCAAT CAAGAGGGGG ATGCCGTCCG CAGGCGCAAG 
CTGGAGCGCA TGAGGGCCGT GCGGCTGCGG GAATGTGGAT TGGAGCCGGG CCGCCGCGGG 
CTGGGCGCGC TGGCCCAGCA GAGCCTCCAT GCCCAGCACG CCCGAGACCT GCAGGTCATT 
GCCGCCTACC GTGAGCGCAC AAAGGCGGAG AGCATCGCCA GCGCACTGAG CACGGCCATC 
ACCACGCAGC ACACCATCCA CGCCACCCTA GGCGCTGCCG AGTTCTTCGA GTTCGCCCTG 
AGGAACCCCC ACAACGCGCA GCACATGGTG ACCATCGAGA TAGACAACCC CGAGCTCAGT 
CTCATCCTGG ATGGTCAGGA GTGGCGGTAC TTCAAGGACA TGGCTGACTT GCACACACCC 
CTGGAGGAGG ACATGTTCCA CCTGCGGGGC AGCCTGGCCC CCCAACTCTT CCTGCGACCT 
CGGGAGACTG CCCACATCCC CTTCAAGTTC CAGACTTTCT CTGTGGGCCC CTGGGCCTCG 
GCACAGGCCT CTGCTGAGCT GAGATCAGAG AAGGACATGG ATGCCGGATC ACCCTATAAG 
TCCAGCATGA TGCCCACCAA ACACGCCAAG GTCCTGTTCC GCGCGGGCAG CGGCAAGCCC 
CTGGCCGTGC TCTGCCTGAC GGTGGAGCTG CAGCCTCACG TGGTGGACCA GGTCTTCCGC 
TTCCACCACC CGGAGCTCAC CTTCCTGAAG AAAGCCATCC GCCTGCCACC CTGGCACACG 
CTTCCAGGTG CCCCTGTAGG GACTTCCAGT GAGGACCCCC CTTTCCATGT TCGATGTAGT 
GACCCGAACA TCATTTGTGA GACACAGAGT GTGGGCCCCG GGGAGCCCCG TGACGTGTTC 
CTGAAGGTGG CCAGTGGCCC TAGCCCAGAG GTCAAGGACT TCTTTGTCAC CATCTACGTA 
GACCGCTGGT TAGCAACACC TGTCCAGACC TGGCAGGTCC ACCTGCACTC GCTGCAGCGA 
GTGGACGTCT CCTGTATCAC TGGCCAGAGG ACCCGCCTGG CTCTCGTCCT CCGGGGGACG 
CAGACAGTTA GGAGAGTGCA GGCTTTCAGC TCACACCCCC AGGAGCTGAC GACGGACCCC 
AGAGGTGTGT TCGTGCTGCC TCCTCACGGG GTGCAGGACT TGCATGTGAG CGTCAGGCCC 
TGCAGGCCGG GCAGCCGCTT CGCGCACCTC CACCTGGTTG ACGTGGACGC CCACCAGCTG 
GTGGCCTCGT GGCTCCTGTG TCTCTCCTGC CGGCCGCCTC TCATCTCCAA GGCCTTCGAG 
ATAAAGCTGC CCGCGGGGAG CGGGAGGGGC GCCAATAAGC GCATCACCTA CACCAATCCC 
TACCCCTCGC GGAGGGCCTA CCGCCTGCAC AGCGACCACC CCCACCTGCT GCAGTTCAAG 
GAGGACGCCT TCCAGGTCGG GGGAGGTGAG ACCTATACCA TCGGCCTGCG GTTTGTGTCC 
CGTCAGAGAG CCGGGGAGGA AGAGATCCTG ATTTACATCA ACGACCACGA AGACAAAAAC 
GAGGAGACCT TTTGCGTGAA GGTCATCTAC CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005217228.1
CDS	281..4576
Translation	MHDWHRIFDQNLLVPPHPQRARQPLKESTAFQCVLKWLDGPLIKQGVLELLSEVGCHLRVSLFDVTYRHFFGRTWRSSPRPAEPLTRQPARIVFNEPLYFHTSLNHPHIVAVVEVVVEGRRRDGTLQTLSCGFGILRIFSHKPESPTSTSQDKRLRLYHGSPRALLHPLLQDPIEQNKHMTLIENCSLQYTLRPHPLLEPVFHLLPENLLVSGLQQIPGLLPAHGESGDALRKPRLQKPLTCYLDDLFFTLYPSLEKFEEELLELVISDCFREAGSPLDVGTLEILERRLCVGVHNGLGFVQRPQVLVLVPELEVALTRSASFSRKVGSSSKASSGNQALVLRSRLRLPEMVRHPAFAIVFQLEYVFNSPSGADSNAASLSSLSSLACMHMVRWAVWNPSLEASSGQVMLPLQGGIQSNPSHCLVYKVPPASISSEEVKQVESGTVQFQYSLSPEEHPGTPTANASSPKAERRSFRKPPVSPSSPPASPSQGLTAAQDSPMGPGLSLSQLAASPPSPAPRRRSAKPSSQPPGSPVPSLVPGEFPLEGRISHLEADLSQTALALGTSAADHLQELPFTPVHAPIIAGTQTRSSGGKLSRASLALLQSAGFPEILDANKQPAEAVSPTDPVKFSPQKEESDCLQGNEIVLQFLAFSRVPQDEQAAPWPQTVYFTFQFYRFPPETTPRLQLVELADAGKTSSASLSHILAQIQKDGSFDAGSPGFQRKYLVDPGFLRPGEQRWFARYLAMQTLQIDVWDGDSLLLIGSAAVQLKHLLRQGRPAVQVSHELEVVATEYEQDAMVVSGDMTKFGCVRPIDVHTVVKGQLHLTLANVGHLCEQRVRSPSSLPPSRSRVISNDGVGNFMGGSLLTPGGPRSVKNVVQAQKLADVDSELAALLFTQPRPGARGPQGINQEGDAVRRRKLERMRAVRLRECGLEPGRRGLGALAQQSLHAQHARDLQVIAAYRERTKAESIASALSTAITTQHTIHATLGAAEFFEFALRNPHNAQHMVTIEIDNPELSLILDGQEWRYFKDMADLHTPLEEDMFHLRGSLAPQLFLRPRETAHIPFKFQTFSVGPWASAQASAELRSEKDMDAGSPYKSSMMPTKHAKVLFRAGSGKPLAVLCLTVELQPHVVDQVFRFHHPELTFLKKAIRLPPWHTLPGAPVGTSSEDPPFHVRCSDPNIICETQSVGPGEPRDVFLKVASGPSPEVKDFFVTIYVDRWLATPVQTWQVHLHSLQRVDVSCITGQRTRLALVLRGTQTVRRVQAFSSHPQELTTDPRGVFVLPPHGVQDLHVSVRPCRPGSRFAHLHLVDVDAHQLVASWLLCLSCRPPLISKAFEIKLPAGSGRGANKRITYTNPYPSRRAYRLHSDHPHLLQFKEDAFQVGGGETYTIGLRFVSRQRAGEEEILIYINDHEDKNEETFCVKVIYQ

Target ORF information:

RefSeq Version	XM_005217171.4
Organism	Bos taurus(cattle)
Definition	Bos taurus nephrocystin 4 (NPHP4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005217171.4

ORF Insert Sequence:

ATGCACGACT GGCACAGGAT CTTTGACCAA AACCTGCTGG TCCCTCCGCA CCCACAGAGA 
GCACGCCAGC CTCTGAAGGA ATCGACGGCG TTTCAGTGTG TCCTGAAGTG GCTTGATGGC 
CCGCTAATTA AGCAGGGGGT GCTGGAGCTG CTATCGGAGG TGGGCTGCCA CCTGCGCGTG 
TCTCTCTTCG ATGTCACCTA TCGGCACTTC TTCGGGAGGA CGTGGAGGAG CTCGCCGAGG 
CCTGCAGAGC CGCTCACCAG GCAGCCGGCC AGGATCGTCT TCAATGAGCC CTTGTATTTT 
CACACGTCCT TGAACCACCC TCATATTGTG GCCGTGGTGG AGGTGGTCGT TGAAGGCAGA 
AGACGAGACG GGACCCTCCA GACTCTGTCC TGTGGGTTTG GAATTCTTCG GATCTTCAGC 
CACAAGCCGG AATCTCCAAC TTCCACTTCC CAGGACAAAC GGCTGAGACT GTATCATGGG 
AGCCCGCGAG CCCTCCTCCA CCCGCTCCTT CAGGACCCCA TAGAACAGAA CAAGCACATG 
ACCCTCATTG AGAACTGCAG CCTGCAGTAC ACGCTGAGGC CACACCCGCT CCTGGAGCCC 
GTGTTCCACC TTCTTCCCGA GAACTTGCTG GTGTCCGGTC TGCAGCAGAT ACCTGGCCTC 
CTTCCAGCTC ACGGGGAATC AGGAGATGCC CTCCGGAAGC CCCGCCTGCA GAAGCCTCTG 
ACGTGCTACC TGGATGATCT GTTCTTCACG CTGTATCCCT CGCTGGAGAA GTTCGAGGAG 
GAGCTTCTGG AACTCGTGAT CAGCGATTGC TTCCGGGAGG CGGGCAGCCC GCTGGACGTC 
GGCACCCTGG AGATCCTGGA GCGGCGCCTG TGCGTGGGCG TGCACAATGG GTTGGGCTTC 
GTGCAGCGGC CGCAGGTCCT CGTGCTGGTG CCCGAGCTGG AGGTGGCCTT GACACGCTCT 
GCCAGCTTCA GCCGGAAGGT CGGCTCCTCT TCCAAGGCCA GCTCTGGAAA CCAGGCGCTG 
GTCTTGAGAA GCCGTCTCCG ACTCCCTGAG ATGGTCCGTC ACCCCGCGTT CGCCATTGTC 
TTCCAGCTGG AGTACGTGTT TAACAGCCCC TCGGGGGCAG ACAGCAATGC AGCTTCACTC 
TCCTCCTTGT CCAGCCTGGC ATGTATGCAC ATGGTTCGCT GGGCTGTCTG GAACCCCTCG 
CTGGAAGCCA GCTCCGGGCA GGTGATGTTA CCTCTCCAGG GTGGCATCCA GTCCAACCCT 
TCGCACTGTC TGGTCTATAA GGTACCCCCA GCCAGCATAA GCTCCGAAGA GGTGAAGCAG 
GTGGAGTCAG GGACGGTCCA GTTCCAGTAC TCGCTGAGCC CTGAAGAACA CCCAGGCACA 
CCCACAGCGA ATGCAAGCAG CCCCAAGGCA GAGCGGCGGT CTTTTAGGAA GCCACCCGTG 
TCCCCTTCAA GTCCACCAGC ATCGCCGTCC CAGGGGCTCA CCGCTGCCCA GGACTCACCC 
ATGGGACCAG GGCTGTCACT TTCTCAGCTG GCGGCTTCCC CACCATCCCC AGCTCCCCGC 
CGCCGCTCGG CCAAACCTTC GTCACAGCCA CCTGGGAGCC CTGTGCCCTC CCTGGTCCCA 
GGGGAGTTCC CCTTGGAGGG CAGGATCTCT CACCTGGAGG CTGACCTGAG CCAGACAGCC 
TTAGCCCTGG GAACGTCCGC TGCTGACCAC TTGCAGGAAC TGCCTTTCAC ACCCGTGCAC 
GCCCCGATCA TTGCAGGAAC CCAGACCAGG AGCTCTGGAG GGAAGCTGTC ACGGGCATCC 
CTGGCGCTGC TGCAGTCCGC TGGCTTTCCC GAGATTCTGG ACGCCAACAA GCAACCAGCT 
GAGGCTGTGA GTCCTACAGA CCCCGTGAAG TTCAGCCCTC AGAAGGAAGA ATCAGACTGT 
CTACAAGGCA ATGAGATAGT GTTGCAATTT CTTGCCTTTA GCAGGGTGCC CCAGGATGAG 
CAGGCAGCGC CATGGCCACA GACTGTGTAC TTCACCTTCC AATTCTACCG CTTCCCACCC 
GAGACCACGC CACGGCTGCA GCTCGTGGAG CTGGCCGATG CTGGGAAGAC CAGCTCTGCC 
TCCCTGTCGC ACATCCTCGC CCAGATCCAG AAGGATGGCT CCTTTGATGC GGGGTCTCCG 
GGCTTTCAGC GGAAGTACCT GGTGGACCCC GGGTTCCTGA GACCCGGGGA GCAGCGCTGG 
TTCGCCCGCT ACCTGGCCAT GCAGACCCTG CAGATCGACG TCTGGGATGG GGACTCCCTG 
CTTCTCATTG GATCTGCTGC CGTTCAGCTG AAGCACCTTC TCCGCCAGGG GCGGCCGGCC 
GTGCAGGTCT CGCATGAGCT GGAGGTTGTG GCGACTGAAT ACGAGCAGGA CGCCATGGTG 
GTGAGTGGAG ACATGACCAA GTTTGGCTGC GTCAGACCCA TCGACGTCCA CACGGTGGTG 
AAGGGCCAGC TGCACTTGAC CTTGGCCAAC GTGGGTCACT TGTGTGAACA GAGAGTAAGG 
AGTCCCAGTT CCCTGCCACC TTCCAGGTCG AGGGTCATCT CAAATGATGG AGTTGGCAAC 
TTCATGGGAG GCAGCCTCCT GACACCCGGG GGCCCCAGAT CAGTGAAAAA TGTGGTCCAA 
GCTCAGAAGC TGGCTGATGT GGACAGCGAA CTGGCCGCCC TGCTCTTCAC ACAACCGAGA 
CCAGGGGCCC GGGGGCCCCA GGGCATCAAT CAAGAGGGGG ATGCCGTCCG CAGGCGCAAG 
CTGGAGCGCA TGAGGGCCGT GCGGCTGCGG GAATGTGGAT TGGAGCCGGG CCGCCGCGGG 
CTGGGCGCGC TGGCCCAGCA GAGCCTCCAT GCCCAGCACG CCCGAGACCT GCAGGTCATT 
GCCGCCTACC GTGAGCGCAC AAAGGCGGAG AGCATCGCCA GCGCACTGAG CACGGCCATC 
ACCACGCAGC ACACCATCCA CGCCACCCTA GGCGCTGCCG AGTTCTTCGA GTTCGCCCTG 
AGGAACCCCC ACAACGCGCA GCACATGGTG ACCATCGAGA TAGACAACCC CGAGCTCAGT 
CTCATCCTGG ATGGTCAGGA GTGGCGGTAC TTCAAGGACA TGGCTGACTT GCACACACCC 
CTGGAGGAGG ACATGTTCCA CCTGCGGGGC AGCCTGGCCC CCCAACTCTT CCTGCGACCT 
CGGGAGACTG CCCACATCCC CTTCAAGTTC CAGACTTTCT CTGTGGGCCC CTGGGCCTCG 
GCACAGGCCT CTGCTGAGCT GAGATCAGAG AAGGACATGG ATGCCGGATC ACCCTATAAG 
TCCAGCATGA TGCCCACCAA ACACGCCAAG GTCCTGTTCC GCGCGGGCAG CGGCAAGCCC 
CTGGCCGTGC TCTGCCTGAC GGTGGAGCTG CAGCCTCACG TGGTGGACCA GGTCTTCCGC 
TTCCACCACC CGGAGCTCAC CTTCCTGAAG AAAGCCATCC GCCTGCCACC CTGGCACACG 
CTTCCAGGTG CCCCTGTAGG GACTTCCAGT GAGGACCCCC CTTTCCATGT TCGATGTAGT 
GACCCGAACA TCATTTGTGA GACACAGAGT GTGGGCCCCG GGGAGCCCCG TGACGTGTTC 
CTGAAGGTGG CCAGTGGCCC TAGCCCAGAG GTCAAGGACT TCTTTGTCAC CATCTACGTA 
GACCGCTGGT TAGCAACACC TGTCCAGACC TGGCAGGTCC ACCTGCACTC GCTGCAGCGA 
GTGGACGTCT CCTGTATCAC TGGCCAGAGG ACCCGCCTGG CTCTCGTCCT CCGGGGGACG 
CAGACAGTTA GGAGAGTGCA GGCTTTCAGC TCACACCCCC AGGAGCTGAC GACGGACCCC 
AGAGGTGTGT TCGTGCTGCC TCCTCACGGG GTGCAGGACT TGCATGTGAG CGTCAGGCCC 
TGCAGGCCGG GCAGCCGCTT CGCGCACCTC CACCTGGTTG ACGTGGACGC CCACCAGCTG 
GTGGCCTCGT GGCTCCTGTG TCTCTCCTGC CGGCCGCCTC TCATCTCCAA GGCCTTCGAG 
ATAAAGCTGC CCGCGGGGAG CGGGAGGGGC GCCAATAAGC GCATCACCTA CACCAATCCC 
TACCCCTCGC GGAGGGCCTA CCGCCTGCAC AGCGACCACC CCCACCTGCT GCAGTTCAAG 
GAGGACGCCT TCCAGGTCGG GGGAGGTGAG ACCTATACCA TCGGCCTGCG GTTTGTGTCC 
CGTCAGAGAG CCGGGGAGGA AGAGATCCTG ATTTACATCA ACGACCACGA AGACAAAAAC 
GAGGAGACCT TTTGCGTGAA GGTCATCTAC CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPHP4 ( XM_015475180.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015330666.1 Bos taurus nephrocystin 4 (NPHP4), transcript variant X3, mRNA.

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CloneID	OBa27367
Clone ID Related Accession (Same CDS sequence)	XM_015475180.1 , XM_015466842.1 , XM_015466845.1 , XM_024976773.1 , XM_005217172.4 , XM_015475179.1 , XM_015466844.1 , XM_015466846.1 , XM_005217172.3 , XM_010813390.2 , XM_024976775.1 , XM_024976776.1 , XM_005217171.3 , XM_024976772.1 , XM_010813390.3 , XM_015466843.1 , XM_005217171.4 , XM_015475180.2 , XM_024976774.1
Accession Version	XM_015475180.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4296bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	nephrocystin-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015475180.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACGACT GGCACAGGAT CTTTGACCAA AACCTGCTGG TCCCTCCGCA CCCACAGAGA 
GCACGCCAGC CTCTGAAGGA ATCGACGGCG TTTCAGTGTG TCCTGAAGTG GCTTGATGGC 
CCGCTAATTA AGCAGGGGGT GCTGGAGCTG CTATCGGAGG TGGGCTGCCA CCTGCGCGTG 
TCTCTCTTCG ATGTCACCTA TCGGCACTTC TTCGGGAGGA CGTGGAGGAG CTCGCCGAGG 
CCTGCAGAGC CGCTCACCAG GCAGCCGGCC AGGATCGTCT TCAATGAGCC CTTGTATTTT 
CACACGTCCT TGAACCACCC TCATATTGTG GCCGTGGTGG AGGTGGTCGT TGAAGGCAGA 
AGACGAGACG GGACCCTCCA GACTCTGTCC TGTGGGTTTG GAATTCTTCG GATCTTCAGC 
CACAAGCCGG AATCTCCAAC TTCCACTTCC CAGGACAAAC GGCTGAGACT GTATCATGGG 
AGCCCGCGAG CCCTCCTCCA CCCGCTCCTT CAGGACCCCA TAGAACAGAA CAAGCACATG 
ACCCTCATTG AGAACTGCAG CCTGCAGTAC ACGCTGAGGC CACACCCGCT CCTGGAGCCC 
GTGTTCCACC TTCTTCCCGA GAACTTGCTG GTGTCCGGTC TGCAGCAGAT ACCTGGCCTC 
CTTCCAGCTC ACGGGGAATC AGGAGATGCC CTCCGGAAGC CCCGCCTGCA GAAGCCTCTG 
ACGTGCTACC TGGATGATCT GTTCTTCACG CTGTATCCCT CGCTGGAGAA GTTCGAGGAG 
GAGCTTCTGG AACTCGTGAT CAGCGATTGC TTCCGGGAGG CGGGCAGCCC GCTGGACGTC 
GGCACCCTGG AGATCCTGGA GCGGCGCCTG TGCGTGGGCG TGCACAATGG GTTGGGCTTC 
GTGCAGCGGC CGCAGGTCCT CGTGCTGGTG CCCGAGCTGG AGGTGGCCTT GACACGCTCT 
GCCAGCTTCA GCCGGAAGGT CGGCTCCTCT TCCAAGGCCA GCTCTGGAAA CCAGGCGCTG 
GTCTTGAGAA GCCGTCTCCG ACTCCCTGAG ATGGTCCGTC ACCCCGCGTT CGCCATTGTC 
TTCCAGCTGG AGTACGTGTT TAACAGCCCC TCGGGGGCAG ACAGCAATGC AGCTTCACTC 
TCCTCCTTGT CCAGCCTGGC ATGTATGCAC ATGGTTCGCT GGGCTGTCTG GAACCCCTCG 
CTGGAAGCCA GCTCCGGGCA GGTGATGTTA CCTCTCCAGG GTGGCATCCA GTCCAACCCT 
TCGCACTGTC TGGTCTATAA GGTACCCCCA GCCAGCATAA GCTCCGAAGA GGTGAAGCAG 
GTGGAGTCAG GGACGGTCCA GTTCCAGTAC TCGCTGAGCC CTGAAGAACA CCCAGGCACA 
CCCACAGCGA ATGCAAGCAG CCCCAAGGCA GAGCGGCGGT CTTTTAGGAA GCCACCCGTG 
TCCCCTTCAA GTCCACCAGC ATCGCCGTCC CAGGGGCTCA CCGCTGCCCA GGACTCACCC 
ATGGGACCAG GGCTGTCACT TTCTCAGCTG GCGGCTTCCC CACCATCCCC AGCTCCCCGC 
CGCCGCTCGG CCAAACCTTC GTCACAGCCA CCTGGGAGCC CTGTGCCCTC CCTGGTCCCA 
GGGGAGTTCC CCTTGGAGGG CAGGATCTCT CACCTGGAGG CTGACCTGAG CCAGACAGCC 
TTAGCCCTGG GAACGTCCGC TGCTGACCAC TTGCAGGAAC TGCCTTTCAC ACCCGTGCAC 
GCCCCGATCA TTGCAGGAAC CCAGACCAGG AGCTCTGGAG GGAAGCTGTC ACGGGCATCC 
CTGGCGCTGC TGCAGTCCGC TGGCTTTCCC GAGATTCTGG ACGCCAACAA GCAACCAGCT 
GAGGCTGTGA GTCCTACAGA CCCCGTGAAG TTCAGCCCTC AGAAGGAAGA ATCAGACTGT 
CTACAAGGCA ATGAGATAGT GTTGCAATTT CTTGCCTTTA GCAGGGTGCC CCAGGATGAG 
CAGGCAGCGC CATGGCCACA GACTGTGTAC TTCACCTTCC AATTCTACCG CTTCCCACCC 
GAGACCACGC CACGGCTGCA GCTCGTGGAG CTGGCCGATG CTGGGAAGAC CAGCTCTGCC 
TCCCTGTCGC ACATCCTCGC CCAGATCCAG AAGGATGGCT CCTTTGATGC GGGGTCTCCG 
GGCTTTCAGC GGAAGTACCT GGTGGACCCC GGGTTCCTGA GACCCGGGGA GCAGCGCTGG 
TTCGCCCGCT ACCTGGCCAT GCAGACCCTG CAGATCGACG TCTGGGATGG GGACTCCCTG 
CTTCTCATTG GATCTGCTGC CGTTCAGCTG AAGCACCTTC TCCGCCAGGG GCGGCCGGCC 
GTGCAGGTCT CGCATGAGCT GGAGGTTGTG GCGACTGAAT ACGAGCAGGA CGCCATGGTG 
GTGAGTGGAG ACATGACCAA GTTTGGCTGC GTCAGACCCA TCGACGTCCA CACGGTGGTG 
AAGGGCCAGC TGCACTTGAC CTTGGCCAAC GTGGGTCACT TGTGTGAACA GAGAGTAAGG 
AGTCCCAGTT CCCTGCCACC TTCCAGGTCG AGGGTCATCT CAAATGATGG AGTTGGCAAC 
TTCATGGGAG GCAGCCTCCT GACACCCGGG GGCCCCAGAT CAGTGAAAAA TGTGGTCCAA 
GCTCAGAAGC TGGCTGATGT GGACAGCGAA CTGGCCGCCC TGCTCTTCAC ACAACCGAGA 
CCAGGGGCCC GGGGGCCCCA GGGCATCAAT CAAGAGGGGG ATGCCGTCCG CAGGCGCAAG 
CTGGAGCGCA TGAGGGCCGT GCGGCTGCGG GAATGTGGAT TGGAGCCGGG CCGCCGCGGG 
CTGGGCGCGC TGGCCCAGCA GAGCCTCCAT GCCCAGCACG CCCGAGACCT GCAGGTCATT 
GCCGCCTACC GTGAGCGCAC AAAGGCGGAG AGCATCGCCA GCGCACTGAG CACGGCCATC 
ACCACGCAGC ACACCATCCA CGCCACCCTA GGCGCTGCCG AGTTCTTCGA GTTCGCCCTG 
AGGAACCCCC ACAACGCGCA GCACATGGTG ACCATCGAGA TAGACAACCC CGAGCTCAGT 
CTCATCCTGG ATGGTCAGGA GTGGCGGTAC TTCAAGGACA TGGCTGACTT GCACACACCC 
CTGGAGGAGG ACATGTTCCA CCTGCGGGGC AGCCTGGCCC CCCAACTCTT CCTGCGACCT 
CGGGAGACTG CCCACATCCC CTTCAAGTTC CAGACTTTCT CTGTGGGCCC CTGGGCCTCG 
GCACAGGCCT CTGCTGAGCT GAGATCAGAG AAGGACATGG ATGCCGGATC ACCCTATAAG 
TCCAGCATGA TGCCCACCAA ACACGCCAAG GTCCTGTTCC GCGCGGGCAG CGGCAAGCCC 
CTGGCCGTGC TCTGCCTGAC GGTGGAGCTG CAGCCTCACG TGGTGGACCA GGTCTTCCGC 
TTCCACCACC CGGAGCTCAC CTTCCTGAAG AAAGCCATCC GCCTGCCACC CTGGCACACG 
CTTCCAGGTG CCCCTGTAGG GACTTCCAGT GAGGACCCCC CTTTCCATGT TCGATGTAGT 
GACCCGAACA TCATTTGTGA GACACAGAGT GTGGGCCCCG GGGAGCCCCG TGACGTGTTC 
CTGAAGGTGG CCAGTGGCCC TAGCCCAGAG GTCAAGGACT TCTTTGTCAC CATCTACGTA 
GACCGCTGGT TAGCAACACC TGTCCAGACC TGGCAGGTCC ACCTGCACTC GCTGCAGCGA 
GTGGACGTCT CCTGTATCAC TGGCCAGAGG ACCCGCCTGG CTCTCGTCCT CCGGGGGACG 
CAGACAGTTA GGAGAGTGCA GGCTTTCAGC TCACACCCCC AGGAGCTGAC GACGGACCCC 
AGAGGTGTGT TCGTGCTGCC TCCTCACGGG GTGCAGGACT TGCATGTGAG CGTCAGGCCC 
TGCAGGCCGG GCAGCCGCTT CGCGCACCTC CACCTGGTTG ACGTGGACGC CCACCAGCTG 
GTGGCCTCGT GGCTCCTGTG TCTCTCCTGC CGGCCGCCTC TCATCTCCAA GGCCTTCGAG 
ATAAAGCTGC CCGCGGGGAG CGGGAGGGGC GCCAATAAGC GCATCACCTA CACCAATCCC 
TACCCCTCGC GGAGGGCCTA CCGCCTGCAC AGCGACCACC CCCACCTGCT GCAGTTCAAG 
GAGGACGCCT TCCAGGTCGG GGGAGGTGAG ACCTATACCA TCGGCCTGCG GTTTGTGTCC 
CGTCAGAGAG CCGGGGAGGA AGAGATCCTG ATTTACATCA ACGACCACGA AGACAAAAAC 
GAGGAGACCT TTTGCGTGAA GGTCATCTAC CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015330666.1
CDS	1219..5514
Translation	MHDWHRIFDQNLLVPPHPQRARQPLKESTAFQCVLKWLDGPLIKQGVLELLSEVGCHLRVSLFDVTYRHFFGRTWRSSPRPAEPLTRQPARIVFNEPLYFHTSLNHPHIVAVVEVVVEGRRRDGTLQTLSCGFGILRIFSHKPESPTSTSQDKRLRLYHGSPRALLHPLLQDPIEQNKHMTLIENCSLQYTLRPHPLLEPVFHLLPENLLVSGLQQIPGLLPAHGESGDALRKPRLQKPLTCYLDDLFFTLYPSLEKFEEELLELVISDCFREAGSPLDVGTLEILERRLCVGVHNGLGFVQRPQVLVLVPELEVALTRSASFSRKVGSSSKASSGNQALVLRSRLRLPEMVRHPAFAIVFQLEYVFNSPSGADSNAASLSSLSSLACMHMVRWAVWNPSLEASSGQVMLPLQGGIQSNPSHCLVYKVPPASISSEEVKQVESGTVQFQYSLSPEEHPGTPTANASSPKAERRSFRKPPVSPSSPPASPSQGLTAAQDSPMGPGLSLSQLAASPPSPAPRRRSAKPSSQPPGSPVPSLVPGEFPLEGRISHLEADLSQTALALGTSAADHLQELPFTPVHAPIIAGTQTRSSGGKLSRASLALLQSAGFPEILDANKQPAEAVSPTDPVKFSPQKEESDCLQGNEIVLQFLAFSRVPQDEQAAPWPQTVYFTFQFYRFPPETTPRLQLVELADAGKTSSASLSHILAQIQKDGSFDAGSPGFQRKYLVDPGFLRPGEQRWFARYLAMQTLQIDVWDGDSLLLIGSAAVQLKHLLRQGRPAVQVSHELEVVATEYEQDAMVVSGDMTKFGCVRPIDVHTVVKGQLHLTLANVGHLCEQRVRSPSSLPPSRSRVISNDGVGNFMGGSLLTPGGPRSVKNVVQAQKLADVDSELAALLFTQPRPGARGPQGINQEGDAVRRRKLERMRAVRLRECGLEPGRRGLGALAQQSLHAQHARDLQVIAAYRERTKAESIASALSTAITTQHTIHATLGAAEFFEFALRNPHNAQHMVTIEIDNPELSLILDGQEWRYFKDMADLHTPLEEDMFHLRGSLAPQLFLRPRETAHIPFKFQTFSVGPWASAQASAELRSEKDMDAGSPYKSSMMPTKHAKVLFRAGSGKPLAVLCLTVELQPHVVDQVFRFHHPELTFLKKAIRLPPWHTLPGAPVGTSSEDPPFHVRCSDPNIICETQSVGPGEPRDVFLKVASGPSPEVKDFFVTIYVDRWLATPVQTWQVHLHSLQRVDVSCITGQRTRLALVLRGTQTVRRVQAFSSHPQELTTDPRGVFVLPPHGVQDLHVSVRPCRPGSRFAHLHLVDVDAHQLVASWLLCLSCRPPLISKAFEIKLPAGSGRGANKRITYTNPYPSRRAYRLHSDHPHLLQFKEDAFQVGGGETYTIGLRFVSRQRAGEEEILIYINDHEDKNEETFCVKVIYQ

Target ORF information:

RefSeq Version	XM_015475180.2
Organism	Bos taurus(cattle)
Definition	Bos taurus nephrocystin 4 (NPHP4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015475180.2

ORF Insert Sequence:

ATGCACGACT GGCACAGGAT CTTTGACCAA AACCTGCTGG TCCCTCCGCA CCCACAGAGA 
GCACGCCAGC CTCTGAAGGA ATCGACGGCG TTTCAGTGTG TCCTGAAGTG GCTTGATGGC 
CCGCTAATTA AGCAGGGGGT GCTGGAGCTG CTATCGGAGG TGGGCTGCCA CCTGCGCGTG 
TCTCTCTTCG ATGTCACCTA TCGGCACTTC TTCGGGAGGA CGTGGAGGAG CTCGCCGAGG 
CCTGCAGAGC CGCTCACCAG GCAGCCGGCC AGGATCGTCT TCAATGAGCC CTTGTATTTT 
CACACGTCCT TGAACCACCC TCATATTGTG GCCGTGGTGG AGGTGGTCGT TGAAGGCAGA 
AGACGAGACG GGACCCTCCA GACTCTGTCC TGTGGGTTTG GAATTCTTCG GATCTTCAGC 
CACAAGCCGG AATCTCCAAC TTCCACTTCC CAGGACAAAC GGCTGAGACT GTATCATGGG 
AGCCCGCGAG CCCTCCTCCA CCCGCTCCTT CAGGACCCCA TAGAACAGAA CAAGCACATG 
ACCCTCATTG AGAACTGCAG CCTGCAGTAC ACGCTGAGGC CACACCCGCT CCTGGAGCCC 
GTGTTCCACC TTCTTCCCGA GAACTTGCTG GTGTCCGGTC TGCAGCAGAT ACCTGGCCTC 
CTTCCAGCTC ACGGGGAATC AGGAGATGCC CTCCGGAAGC CCCGCCTGCA GAAGCCTCTG 
ACGTGCTACC TGGATGATCT GTTCTTCACG CTGTATCCCT CGCTGGAGAA GTTCGAGGAG 
GAGCTTCTGG AACTCGTGAT CAGCGATTGC TTCCGGGAGG CGGGCAGCCC GCTGGACGTC 
GGCACCCTGG AGATCCTGGA GCGGCGCCTG TGCGTGGGCG TGCACAATGG GTTGGGCTTC 
GTGCAGCGGC CGCAGGTCCT CGTGCTGGTG CCCGAGCTGG AGGTGGCCTT GACACGCTCT 
GCCAGCTTCA GCCGGAAGGT CGGCTCCTCT TCCAAGGCCA GCTCTGGAAA CCAGGCGCTG 
GTCTTGAGAA GCCGTCTCCG ACTCCCTGAG ATGGTCCGTC ACCCCGCGTT CGCCATTGTC 
TTCCAGCTGG AGTACGTGTT TAACAGCCCC TCGGGGGCAG ACAGCAATGC AGCTTCACTC 
TCCTCCTTGT CCAGCCTGGC ATGTATGCAC ATGGTTCGCT GGGCTGTCTG GAACCCCTCG 
CTGGAAGCCA GCTCCGGGCA GGTGATGTTA CCTCTCCAGG GTGGCATCCA GTCCAACCCT 
TCGCACTGTC TGGTCTATAA GGTACCCCCA GCCAGCATAA GCTCCGAAGA GGTGAAGCAG 
GTGGAGTCAG GGACGGTCCA GTTCCAGTAC TCGCTGAGCC CTGAAGAACA CCCAGGCACA 
CCCACAGCGA ATGCAAGCAG CCCCAAGGCA GAGCGGCGGT CTTTTAGGAA GCCACCCGTG 
TCCCCTTCAA GTCCACCAGC ATCGCCGTCC CAGGGGCTCA CCGCTGCCCA GGACTCACCC 
ATGGGACCAG GGCTGTCACT TTCTCAGCTG GCGGCTTCCC CACCATCCCC AGCTCCCCGC 
CGCCGCTCGG CCAAACCTTC GTCACAGCCA CCTGGGAGCC CTGTGCCCTC CCTGGTCCCA 
GGGGAGTTCC CCTTGGAGGG CAGGATCTCT CACCTGGAGG CTGACCTGAG CCAGACAGCC 
TTAGCCCTGG GAACGTCCGC TGCTGACCAC TTGCAGGAAC TGCCTTTCAC ACCCGTGCAC 
GCCCCGATCA TTGCAGGAAC CCAGACCAGG AGCTCTGGAG GGAAGCTGTC ACGGGCATCC 
CTGGCGCTGC TGCAGTCCGC TGGCTTTCCC GAGATTCTGG ACGCCAACAA GCAACCAGCT 
GAGGCTGTGA GTCCTACAGA CCCCGTGAAG TTCAGCCCTC AGAAGGAAGA ATCAGACTGT 
CTACAAGGCA ATGAGATAGT GTTGCAATTT CTTGCCTTTA GCAGGGTGCC CCAGGATGAG 
CAGGCAGCGC CATGGCCACA GACTGTGTAC TTCACCTTCC AATTCTACCG CTTCCCACCC 
GAGACCACGC CACGGCTGCA GCTCGTGGAG CTGGCCGATG CTGGGAAGAC CAGCTCTGCC 
TCCCTGTCGC ACATCCTCGC CCAGATCCAG AAGGATGGCT CCTTTGATGC GGGGTCTCCG 
GGCTTTCAGC GGAAGTACCT GGTGGACCCC GGGTTCCTGA GACCCGGGGA GCAGCGCTGG 
TTCGCCCGCT ACCTGGCCAT GCAGACCCTG CAGATCGACG TCTGGGATGG GGACTCCCTG 
CTTCTCATTG GATCTGCTGC CGTTCAGCTG AAGCACCTTC TCCGCCAGGG GCGGCCGGCC 
GTGCAGGTCT CGCATGAGCT GGAGGTTGTG GCGACTGAAT ACGAGCAGGA CGCCATGGTG 
GTGAGTGGAG ACATGACCAA GTTTGGCTGC GTCAGACCCA TCGACGTCCA CACGGTGGTG 
AAGGGCCAGC TGCACTTGAC CTTGGCCAAC GTGGGTCACT TGTGTGAACA GAGAGTAAGG 
AGTCCCAGTT CCCTGCCACC TTCCAGGTCG AGGGTCATCT CAAATGATGG AGTTGGCAAC 
TTCATGGGAG GCAGCCTCCT GACACCCGGG GGCCCCAGAT CAGTGAAAAA TGTGGTCCAA 
GCTCAGAAGC TGGCTGATGT GGACAGCGAA CTGGCCGCCC TGCTCTTCAC ACAACCGAGA 
CCAGGGGCCC GGGGGCCCCA GGGCATCAAT CAAGAGGGGG ATGCCGTCCG CAGGCGCAAG 
CTGGAGCGCA TGAGGGCCGT GCGGCTGCGG GAATGTGGAT TGGAGCCGGG CCGCCGCGGG 
CTGGGCGCGC TGGCCCAGCA GAGCCTCCAT GCCCAGCACG CCCGAGACCT GCAGGTCATT 
GCCGCCTACC GTGAGCGCAC AAAGGCGGAG AGCATCGCCA GCGCACTGAG CACGGCCATC 
ACCACGCAGC ACACCATCCA CGCCACCCTA GGCGCTGCCG AGTTCTTCGA GTTCGCCCTG 
AGGAACCCCC ACAACGCGCA GCACATGGTG ACCATCGAGA TAGACAACCC CGAGCTCAGT 
CTCATCCTGG ATGGTCAGGA GTGGCGGTAC TTCAAGGACA TGGCTGACTT GCACACACCC 
CTGGAGGAGG ACATGTTCCA CCTGCGGGGC AGCCTGGCCC CCCAACTCTT CCTGCGACCT 
CGGGAGACTG CCCACATCCC CTTCAAGTTC CAGACTTTCT CTGTGGGCCC CTGGGCCTCG 
GCACAGGCCT CTGCTGAGCT GAGATCAGAG AAGGACATGG ATGCCGGATC ACCCTATAAG 
TCCAGCATGA TGCCCACCAA ACACGCCAAG GTCCTGTTCC GCGCGGGCAG CGGCAAGCCC 
CTGGCCGTGC TCTGCCTGAC GGTGGAGCTG CAGCCTCACG TGGTGGACCA GGTCTTCCGC 
TTCCACCACC CGGAGCTCAC CTTCCTGAAG AAAGCCATCC GCCTGCCACC CTGGCACACG 
CTTCCAGGTG CCCCTGTAGG GACTTCCAGT GAGGACCCCC CTTTCCATGT TCGATGTAGT 
GACCCGAACA TCATTTGTGA GACACAGAGT GTGGGCCCCG GGGAGCCCCG TGACGTGTTC 
CTGAAGGTGG CCAGTGGCCC TAGCCCAGAG GTCAAGGACT TCTTTGTCAC CATCTACGTA 
GACCGCTGGT TAGCAACACC TGTCCAGACC TGGCAGGTCC ACCTGCACTC GCTGCAGCGA 
GTGGACGTCT CCTGTATCAC TGGCCAGAGG ACCCGCCTGG CTCTCGTCCT CCGGGGGACG 
CAGACAGTTA GGAGAGTGCA GGCTTTCAGC TCACACCCCC AGGAGCTGAC GACGGACCCC 
AGAGGTGTGT TCGTGCTGCC TCCTCACGGG GTGCAGGACT TGCATGTGAG CGTCAGGCCC 
TGCAGGCCGG GCAGCCGCTT CGCGCACCTC CACCTGGTTG ACGTGGACGC CCACCAGCTG 
GTGGCCTCGT GGCTCCTGTG TCTCTCCTGC CGGCCGCCTC TCATCTCCAA GGCCTTCGAG 
ATAAAGCTGC CCGCGGGGAG CGGGAGGGGC GCCAATAAGC GCATCACCTA CACCAATCCC 
TACCCCTCGC GGAGGGCCTA CCGCCTGCAC AGCGACCACC CCCACCTGCT GCAGTTCAAG 
GAGGACGCCT TCCAGGTCGG GGGAGGTGAG ACCTATACCA TCGGCCTGCG GTTTGTGTCC 
CGTCAGAGAG CCGGGGAGGA AGAGATCCTG ATTTACATCA ACGACCACGA AGACAAAAAC 
GAGGAGACCT TTTGCGTGAA GGTCATCTAC CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPHP4 ( XM_024976774.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832542.1 Bos taurus nephrocystin 4 (NPHP4), transcript variant X7, mRNA.

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CloneID	OBa27367
Clone ID Related Accession (Same CDS sequence)	XM_015475180.1 , XM_015466842.1 , XM_015466845.1 , XM_024976773.1 , XM_005217172.4 , XM_015475179.1 , XM_015466844.1 , XM_015466846.1 , XM_005217172.3 , XM_010813390.2 , XM_024976775.1 , XM_024976776.1 , XM_005217171.3 , XM_024976772.1 , XM_010813390.3 , XM_015466843.1 , XM_005217171.4 , XM_015475180.2 , XM_024976774.1
Accession Version	XM_024976774.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4296bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	nephrocystin-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACGACT GGCACAGGAT CTTTGACCAA AACCTGCTGG TCCCTCCGCA CCCACAGAGA 
GCACGCCAGC CTCTGAAGGA ATCGACGGCG TTTCAGTGTG TCCTGAAGTG GCTTGATGGC 
CCGCTAATTA AGCAGGGGGT GCTGGAGCTG CTATCGGAGG TGGGCTGCCA CCTGCGCGTG 
TCTCTCTTCG ATGTCACCTA TCGGCACTTC TTCGGGAGGA CGTGGAGGAG CTCGCCGAGG 
CCTGCAGAGC CGCTCACCAG GCAGCCGGCC AGGATCGTCT TCAATGAGCC CTTGTATTTT 
CACACGTCCT TGAACCACCC TCATATTGTG GCCGTGGTGG AGGTGGTCGT TGAAGGCAGA 
AGACGAGACG GGACCCTCCA GACTCTGTCC TGTGGGTTTG GAATTCTTCG GATCTTCAGC 
CACAAGCCGG AATCTCCAAC TTCCACTTCC CAGGACAAAC GGCTGAGACT GTATCATGGG 
AGCCCGCGAG CCCTCCTCCA CCCGCTCCTT CAGGACCCCA TAGAACAGAA CAAGCACATG 
ACCCTCATTG AGAACTGCAG CCTGCAGTAC ACGCTGAGGC CACACCCGCT CCTGGAGCCC 
GTGTTCCACC TTCTTCCCGA GAACTTGCTG GTGTCCGGTC TGCAGCAGAT ACCTGGCCTC 
CTTCCAGCTC ACGGGGAATC AGGAGATGCC CTCCGGAAGC CCCGCCTGCA GAAGCCTCTG 
ACGTGCTACC TGGATGATCT GTTCTTCACG CTGTATCCCT CGCTGGAGAA GTTCGAGGAG 
GAGCTTCTGG AACTCGTGAT CAGCGATTGC TTCCGGGAGG CGGGCAGCCC GCTGGACGTC 
GGCACCCTGG AGATCCTGGA GCGGCGCCTG TGCGTGGGCG TGCACAATGG GTTGGGCTTC 
GTGCAGCGGC CGCAGGTCCT CGTGCTGGTG CCCGAGCTGG AGGTGGCCTT GACACGCTCT 
GCCAGCTTCA GCCGGAAGGT CGGCTCCTCT TCCAAGGCCA GCTCTGGAAA CCAGGCGCTG 
GTCTTGAGAA GCCGTCTCCG ACTCCCTGAG ATGGTCCGTC ACCCCGCGTT CGCCATTGTC 
TTCCAGCTGG AGTACGTGTT TAACAGCCCC TCGGGGGCAG ACAGCAATGC AGCTTCACTC 
TCCTCCTTGT CCAGCCTGGC ATGTATGCAC ATGGTTCGCT GGGCTGTCTG GAACCCCTCG 
CTGGAAGCCA GCTCCGGGCA GGTGATGTTA CCTCTCCAGG GTGGCATCCA GTCCAACCCT 
TCGCACTGTC TGGTCTATAA GGTACCCCCA GCCAGCATAA GCTCCGAAGA GGTGAAGCAG 
GTGGAGTCAG GGACGGTCCA GTTCCAGTAC TCGCTGAGCC CTGAAGAACA CCCAGGCACA 
CCCACAGCGA ATGCAAGCAG CCCCAAGGCA GAGCGGCGGT CTTTTAGGAA GCCACCCGTG 
TCCCCTTCAA GTCCACCAGC ATCGCCGTCC CAGGGGCTCA CCGCTGCCCA GGACTCACCC 
ATGGGACCAG GGCTGTCACT TTCTCAGCTG GCGGCTTCCC CACCATCCCC AGCTCCCCGC 
CGCCGCTCGG CCAAACCTTC GTCACAGCCA CCTGGGAGCC CTGTGCCCTC CCTGGTCCCA 
GGGGAGTTCC CCTTGGAGGG CAGGATCTCT CACCTGGAGG CTGACCTGAG CCAGACAGCC 
TTAGCCCTGG GAACGTCCGC TGCTGACCAC TTGCAGGAAC TGCCTTTCAC ACCCGTGCAC 
GCCCCGATCA TTGCAGGAAC CCAGACCAGG AGCTCTGGAG GGAAGCTGTC ACGGGCATCC 
CTGGCGCTGC TGCAGTCCGC TGGCTTTCCC GAGATTCTGG ACGCCAACAA GCAACCAGCT 
GAGGCTGTGA GTCCTACAGA CCCCGTGAAG TTCAGCCCTC AGAAGGAAGA ATCAGACTGT 
CTACAAGGCA ATGAGATAGT GTTGCAATTT CTTGCCTTTA GCAGGGTGCC CCAGGATGAG 
CAGGCAGCGC CATGGCCACA GACTGTGTAC TTCACCTTCC AATTCTACCG CTTCCCACCC 
GAGACCACGC CACGGCTGCA GCTCGTGGAG CTGGCCGATG CTGGGAAGAC CAGCTCTGCC 
TCCCTGTCGC ACATCCTCGC CCAGATCCAG AAGGATGGCT CCTTTGATGC GGGGTCTCCG 
GGCTTTCAGC GGAAGTACCT GGTGGACCCC GGGTTCCTGA GACCCGGGGA GCAGCGCTGG 
TTCGCCCGCT ACCTGGCCAT GCAGACCCTG CAGATCGACG TCTGGGATGG GGACTCCCTG 
CTTCTCATTG GATCTGCTGC CGTTCAGCTG AAGCACCTTC TCCGCCAGGG GCGGCCGGCC 
GTGCAGGTCT CGCATGAGCT GGAGGTTGTG GCGACTGAAT ACGAGCAGGA CGCCATGGTG 
GTGAGTGGAG ACATGACCAA GTTTGGCTGC GTCAGACCCA TCGACGTCCA CACGGTGGTG 
AAGGGCCAGC TGCACTTGAC CTTGGCCAAC GTGGGTCACT TGTGTGAACA GAGAGTAAGG 
AGTCCCAGTT CCCTGCCACC TTCCAGGTCG AGGGTCATCT CAAATGATGG AGTTGGCAAC 
TTCATGGGAG GCAGCCTCCT GACACCCGGG GGCCCCAGAT CAGTGAAAAA TGTGGTCCAA 
GCTCAGAAGC TGGCTGATGT GGACAGCGAA CTGGCCGCCC TGCTCTTCAC ACAACCGAGA 
CCAGGGGCCC GGGGGCCCCA GGGCATCAAT CAAGAGGGGG ATGCCGTCCG CAGGCGCAAG 
CTGGAGCGCA TGAGGGCCGT GCGGCTGCGG GAATGTGGAT TGGAGCCGGG CCGCCGCGGG 
CTGGGCGCGC TGGCCCAGCA GAGCCTCCAT GCCCAGCACG CCCGAGACCT GCAGGTCATT 
GCCGCCTACC GTGAGCGCAC AAAGGCGGAG AGCATCGCCA GCGCACTGAG CACGGCCATC 
ACCACGCAGC ACACCATCCA CGCCACCCTA GGCGCTGCCG AGTTCTTCGA GTTCGCCCTG 
AGGAACCCCC ACAACGCGCA GCACATGGTG ACCATCGAGA TAGACAACCC CGAGCTCAGT 
CTCATCCTGG ATGGTCAGGA GTGGCGGTAC TTCAAGGACA TGGCTGACTT GCACACACCC 
CTGGAGGAGG ACATGTTCCA CCTGCGGGGC AGCCTGGCCC CCCAACTCTT CCTGCGACCT 
CGGGAGACTG CCCACATCCC CTTCAAGTTC CAGACTTTCT CTGTGGGCCC CTGGGCCTCG 
GCACAGGCCT CTGCTGAGCT GAGATCAGAG AAGGACATGG ATGCCGGATC ACCCTATAAG 
TCCAGCATGA TGCCCACCAA ACACGCCAAG GTCCTGTTCC GCGCGGGCAG CGGCAAGCCC 
CTGGCCGTGC TCTGCCTGAC GGTGGAGCTG CAGCCTCACG TGGTGGACCA GGTCTTCCGC 
TTCCACCACC CGGAGCTCAC CTTCCTGAAG AAAGCCATCC GCCTGCCACC CTGGCACACG 
CTTCCAGGTG CCCCTGTAGG GACTTCCAGT GAGGACCCCC CTTTCCATGT TCGATGTAGT 
GACCCGAACA TCATTTGTGA GACACAGAGT GTGGGCCCCG GGGAGCCCCG TGACGTGTTC 
CTGAAGGTGG CCAGTGGCCC TAGCCCAGAG GTCAAGGACT TCTTTGTCAC CATCTACGTA 
GACCGCTGGT TAGCAACACC TGTCCAGACC TGGCAGGTCC ACCTGCACTC GCTGCAGCGA 
GTGGACGTCT CCTGTATCAC TGGCCAGAGG ACCCGCCTGG CTCTCGTCCT CCGGGGGACG 
CAGACAGTTA GGAGAGTGCA GGCTTTCAGC TCACACCCCC AGGAGCTGAC GACGGACCCC 
AGAGGTGTGT TCGTGCTGCC TCCTCACGGG GTGCAGGACT TGCATGTGAG CGTCAGGCCC 
TGCAGGCCGG GCAGCCGCTT CGCGCACCTC CACCTGGTTG ACGTGGACGC CCACCAGCTG 
GTGGCCTCGT GGCTCCTGTG TCTCTCCTGC CGGCCGCCTC TCATCTCCAA GGCCTTCGAG 
ATAAAGCTGC CCGCGGGGAG CGGGAGGGGC GCCAATAAGC GCATCACCTA CACCAATCCC 
TACCCCTCGC GGAGGGCCTA CCGCCTGCAC AGCGACCACC CCCACCTGCT GCAGTTCAAG 
GAGGACGCCT TCCAGGTCGG GGGAGGTGAG ACCTATACCA TCGGCCTGCG GTTTGTGTCC 
CGTCAGAGAG CCGGGGAGGA AGAGATCCTG ATTTACATCA ACGACCACGA AGACAAAAAC 
GAGGAGACCT TTTGCGTGAA GGTCATCTAC CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832542.1
CDS	408..4703
Translation	MHDWHRIFDQNLLVPPHPQRARQPLKESTAFQCVLKWLDGPLIKQGVLELLSEVGCHLRVSLFDVTYRHFFGRTWRSSPRPAEPLTRQPARIVFNEPLYFHTSLNHPHIVAVVEVVVEGRRRDGTLQTLSCGFGILRIFSHKPESPTSTSQDKRLRLYHGSPRALLHPLLQDPIEQNKHMTLIENCSLQYTLRPHPLLEPVFHLLPENLLVSGLQQIPGLLPAHGESGDALRKPRLQKPLTCYLDDLFFTLYPSLEKFEEELLELVISDCFREAGSPLDVGTLEILERRLCVGVHNGLGFVQRPQVLVLVPELEVALTRSASFSRKVGSSSKASSGNQALVLRSRLRLPEMVRHPAFAIVFQLEYVFNSPSGADSNAASLSSLSSLACMHMVRWAVWNPSLEASSGQVMLPLQGGIQSNPSHCLVYKVPPASISSEEVKQVESGTVQFQYSLSPEEHPGTPTANASSPKAERRSFRKPPVSPSSPPASPSQGLTAAQDSPMGPGLSLSQLAASPPSPAPRRRSAKPSSQPPGSPVPSLVPGEFPLEGRISHLEADLSQTALALGTSAADHLQELPFTPVHAPIIAGTQTRSSGGKLSRASLALLQSAGFPEILDANKQPAEAVSPTDPVKFSPQKEESDCLQGNEIVLQFLAFSRVPQDEQAAPWPQTVYFTFQFYRFPPETTPRLQLVELADAGKTSSASLSHILAQIQKDGSFDAGSPGFQRKYLVDPGFLRPGEQRWFARYLAMQTLQIDVWDGDSLLLIGSAAVQLKHLLRQGRPAVQVSHELEVVATEYEQDAMVVSGDMTKFGCVRPIDVHTVVKGQLHLTLANVGHLCEQRVRSPSSLPPSRSRVISNDGVGNFMGGSLLTPGGPRSVKNVVQAQKLADVDSELAALLFTQPRPGARGPQGINQEGDAVRRRKLERMRAVRLRECGLEPGRRGLGALAQQSLHAQHARDLQVIAAYRERTKAESIASALSTAITTQHTIHATLGAAEFFEFALRNPHNAQHMVTIEIDNPELSLILDGQEWRYFKDMADLHTPLEEDMFHLRGSLAPQLFLRPRETAHIPFKFQTFSVGPWASAQASAELRSEKDMDAGSPYKSSMMPTKHAKVLFRAGSGKPLAVLCLTVELQPHVVDQVFRFHHPELTFLKKAIRLPPWHTLPGAPVGTSSEDPPFHVRCSDPNIICETQSVGPGEPRDVFLKVASGPSPEVKDFFVTIYVDRWLATPVQTWQVHLHSLQRVDVSCITGQRTRLALVLRGTQTVRRVQAFSSHPQELTTDPRGVFVLPPHGVQDLHVSVRPCRPGSRFAHLHLVDVDAHQLVASWLLCLSCRPPLISKAFEIKLPAGSGRGANKRITYTNPYPSRRAYRLHSDHPHLLQFKEDAFQVGGGETYTIGLRFVSRQRAGEEEILIYINDHEDKNEETFCVKVIYQ

Target ORF information:

RefSeq Version	XM_024976774.1
Organism	Bos taurus(cattle)
Definition	Bos taurus nephrocystin 4 (NPHP4), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976774.1

ORF Insert Sequence:

ATGCACGACT GGCACAGGAT CTTTGACCAA AACCTGCTGG TCCCTCCGCA CCCACAGAGA 
GCACGCCAGC CTCTGAAGGA ATCGACGGCG TTTCAGTGTG TCCTGAAGTG GCTTGATGGC 
CCGCTAATTA AGCAGGGGGT GCTGGAGCTG CTATCGGAGG TGGGCTGCCA CCTGCGCGTG 
TCTCTCTTCG ATGTCACCTA TCGGCACTTC TTCGGGAGGA CGTGGAGGAG CTCGCCGAGG 
CCTGCAGAGC CGCTCACCAG GCAGCCGGCC AGGATCGTCT TCAATGAGCC CTTGTATTTT 
CACACGTCCT TGAACCACCC TCATATTGTG GCCGTGGTGG AGGTGGTCGT TGAAGGCAGA 
AGACGAGACG GGACCCTCCA GACTCTGTCC TGTGGGTTTG GAATTCTTCG GATCTTCAGC 
CACAAGCCGG AATCTCCAAC TTCCACTTCC CAGGACAAAC GGCTGAGACT GTATCATGGG 
AGCCCGCGAG CCCTCCTCCA CCCGCTCCTT CAGGACCCCA TAGAACAGAA CAAGCACATG 
ACCCTCATTG AGAACTGCAG CCTGCAGTAC ACGCTGAGGC CACACCCGCT CCTGGAGCCC 
GTGTTCCACC TTCTTCCCGA GAACTTGCTG GTGTCCGGTC TGCAGCAGAT ACCTGGCCTC 
CTTCCAGCTC ACGGGGAATC AGGAGATGCC CTCCGGAAGC CCCGCCTGCA GAAGCCTCTG 
ACGTGCTACC TGGATGATCT GTTCTTCACG CTGTATCCCT CGCTGGAGAA GTTCGAGGAG 
GAGCTTCTGG AACTCGTGAT CAGCGATTGC TTCCGGGAGG CGGGCAGCCC GCTGGACGTC 
GGCACCCTGG AGATCCTGGA GCGGCGCCTG TGCGTGGGCG TGCACAATGG GTTGGGCTTC 
GTGCAGCGGC CGCAGGTCCT CGTGCTGGTG CCCGAGCTGG AGGTGGCCTT GACACGCTCT 
GCCAGCTTCA GCCGGAAGGT CGGCTCCTCT TCCAAGGCCA GCTCTGGAAA CCAGGCGCTG 
GTCTTGAGAA GCCGTCTCCG ACTCCCTGAG ATGGTCCGTC ACCCCGCGTT CGCCATTGTC 
TTCCAGCTGG AGTACGTGTT TAACAGCCCC TCGGGGGCAG ACAGCAATGC AGCTTCACTC 
TCCTCCTTGT CCAGCCTGGC ATGTATGCAC ATGGTTCGCT GGGCTGTCTG GAACCCCTCG 
CTGGAAGCCA GCTCCGGGCA GGTGATGTTA CCTCTCCAGG GTGGCATCCA GTCCAACCCT 
TCGCACTGTC TGGTCTATAA GGTACCCCCA GCCAGCATAA GCTCCGAAGA GGTGAAGCAG 
GTGGAGTCAG GGACGGTCCA GTTCCAGTAC TCGCTGAGCC CTGAAGAACA CCCAGGCACA 
CCCACAGCGA ATGCAAGCAG CCCCAAGGCA GAGCGGCGGT CTTTTAGGAA GCCACCCGTG 
TCCCCTTCAA GTCCACCAGC ATCGCCGTCC CAGGGGCTCA CCGCTGCCCA GGACTCACCC 
ATGGGACCAG GGCTGTCACT TTCTCAGCTG GCGGCTTCCC CACCATCCCC AGCTCCCCGC 
CGCCGCTCGG CCAAACCTTC GTCACAGCCA CCTGGGAGCC CTGTGCCCTC CCTGGTCCCA 
GGGGAGTTCC CCTTGGAGGG CAGGATCTCT CACCTGGAGG CTGACCTGAG CCAGACAGCC 
TTAGCCCTGG GAACGTCCGC TGCTGACCAC TTGCAGGAAC TGCCTTTCAC ACCCGTGCAC 
GCCCCGATCA TTGCAGGAAC CCAGACCAGG AGCTCTGGAG GGAAGCTGTC ACGGGCATCC 
CTGGCGCTGC TGCAGTCCGC TGGCTTTCCC GAGATTCTGG ACGCCAACAA GCAACCAGCT 
GAGGCTGTGA GTCCTACAGA CCCCGTGAAG TTCAGCCCTC AGAAGGAAGA ATCAGACTGT 
CTACAAGGCA ATGAGATAGT GTTGCAATTT CTTGCCTTTA GCAGGGTGCC CCAGGATGAG 
CAGGCAGCGC CATGGCCACA GACTGTGTAC TTCACCTTCC AATTCTACCG CTTCCCACCC 
GAGACCACGC CACGGCTGCA GCTCGTGGAG CTGGCCGATG CTGGGAAGAC CAGCTCTGCC 
TCCCTGTCGC ACATCCTCGC CCAGATCCAG AAGGATGGCT CCTTTGATGC GGGGTCTCCG 
GGCTTTCAGC GGAAGTACCT GGTGGACCCC GGGTTCCTGA GACCCGGGGA GCAGCGCTGG 
TTCGCCCGCT ACCTGGCCAT GCAGACCCTG CAGATCGACG TCTGGGATGG GGACTCCCTG 
CTTCTCATTG GATCTGCTGC CGTTCAGCTG AAGCACCTTC TCCGCCAGGG GCGGCCGGCC 
GTGCAGGTCT CGCATGAGCT GGAGGTTGTG GCGACTGAAT ACGAGCAGGA CGCCATGGTG 
GTGAGTGGAG ACATGACCAA GTTTGGCTGC GTCAGACCCA TCGACGTCCA CACGGTGGTG 
AAGGGCCAGC TGCACTTGAC CTTGGCCAAC GTGGGTCACT TGTGTGAACA GAGAGTAAGG 
AGTCCCAGTT CCCTGCCACC TTCCAGGTCG AGGGTCATCT CAAATGATGG AGTTGGCAAC 
TTCATGGGAG GCAGCCTCCT GACACCCGGG GGCCCCAGAT CAGTGAAAAA TGTGGTCCAA 
GCTCAGAAGC TGGCTGATGT GGACAGCGAA CTGGCCGCCC TGCTCTTCAC ACAACCGAGA 
CCAGGGGCCC GGGGGCCCCA GGGCATCAAT CAAGAGGGGG ATGCCGTCCG CAGGCGCAAG 
CTGGAGCGCA TGAGGGCCGT GCGGCTGCGG GAATGTGGAT TGGAGCCGGG CCGCCGCGGG 
CTGGGCGCGC TGGCCCAGCA GAGCCTCCAT GCCCAGCACG CCCGAGACCT GCAGGTCATT 
GCCGCCTACC GTGAGCGCAC AAAGGCGGAG AGCATCGCCA GCGCACTGAG CACGGCCATC 
ACCACGCAGC ACACCATCCA CGCCACCCTA GGCGCTGCCG AGTTCTTCGA GTTCGCCCTG 
AGGAACCCCC ACAACGCGCA GCACATGGTG ACCATCGAGA TAGACAACCC CGAGCTCAGT 
CTCATCCTGG ATGGTCAGGA GTGGCGGTAC TTCAAGGACA TGGCTGACTT GCACACACCC 
CTGGAGGAGG ACATGTTCCA CCTGCGGGGC AGCCTGGCCC CCCAACTCTT CCTGCGACCT 
CGGGAGACTG CCCACATCCC CTTCAAGTTC CAGACTTTCT CTGTGGGCCC CTGGGCCTCG 
GCACAGGCCT CTGCTGAGCT GAGATCAGAG AAGGACATGG ATGCCGGATC ACCCTATAAG 
TCCAGCATGA TGCCCACCAA ACACGCCAAG GTCCTGTTCC GCGCGGGCAG CGGCAAGCCC 
CTGGCCGTGC TCTGCCTGAC GGTGGAGCTG CAGCCTCACG TGGTGGACCA GGTCTTCCGC 
TTCCACCACC CGGAGCTCAC CTTCCTGAAG AAAGCCATCC GCCTGCCACC CTGGCACACG 
CTTCCAGGTG CCCCTGTAGG GACTTCCAGT GAGGACCCCC CTTTCCATGT TCGATGTAGT 
GACCCGAACA TCATTTGTGA GACACAGAGT GTGGGCCCCG GGGAGCCCCG TGACGTGTTC 
CTGAAGGTGG CCAGTGGCCC TAGCCCAGAG GTCAAGGACT TCTTTGTCAC CATCTACGTA 
GACCGCTGGT TAGCAACACC TGTCCAGACC TGGCAGGTCC ACCTGCACTC GCTGCAGCGA 
GTGGACGTCT CCTGTATCAC TGGCCAGAGG ACCCGCCTGG CTCTCGTCCT CCGGGGGACG 
CAGACAGTTA GGAGAGTGCA GGCTTTCAGC TCACACCCCC AGGAGCTGAC GACGGACCCC 
AGAGGTGTGT TCGTGCTGCC TCCTCACGGG GTGCAGGACT TGCATGTGAG CGTCAGGCCC 
TGCAGGCCGG GCAGCCGCTT CGCGCACCTC CACCTGGTTG ACGTGGACGC CCACCAGCTG 
GTGGCCTCGT GGCTCCTGTG TCTCTCCTGC CGGCCGCCTC TCATCTCCAA GGCCTTCGAG 
ATAAAGCTGC CCGCGGGGAG CGGGAGGGGC GCCAATAAGC GCATCACCTA CACCAATCCC 
TACCCCTCGC GGAGGGCCTA CCGCCTGCAC AGCGACCACC CCCACCTGCT GCAGTTCAAG 
GAGGACGCCT TCCAGGTCGG GGGAGGTGAG ACCTATACCA TCGGCCTGCG GTTTGTGTCC 
CGTCAGAGAG CCGGGGAGGA AGAGATCCTG ATTTACATCA ACGACCACGA AGACAAAAAC 
GAGGAGACCT TTTGCGTGAA GGTCATCTAC CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPHP4 ( XM_015466841.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015322327.1 Bos taurus nephronophthisis 4 (NPHP4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa50068
Clone ID Related Accession (Same CDS sequence)	XM_015466841.1 , XM_010813384.2
Accession Version	XM_015466841.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4527bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	nephrocystin-4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646324.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGTGC CGTCGCCGCC AGTTCCAAGC CGGGTCTCCA TCACAGCTCC CTGCACCCTC 
CTCTCCTCTG TGATTTTCAT AGGTCACAAC CACCTCTTTT GTGTGCTAAC TTGTATCCTG 
TGTTTCTGCC TCCCTTCAGA TACATTCCTT GAGGTCATCG TGGTTGTCTG CAGTGTTCTC 
CAGGGCCTGG CACAGGGTAA TCCCAGAGGC CCCAGGGACC CGGGGACCAG CAGAATGCAC 
GACTGGCACA GGATCTTTGA CCAAAACCTG CTGGTCCCTC CGCACCCACA GAGAGCACGC 
CAGCCTCTGA AGGAATCGAC GGCGTTTCAG TGTGTCCTGA AGTGGCTTGA TGGCCCGCTA 
ATTAAGCAGG GGGTGCTGGA GCTGCTATCG GAGGTGGGCT GCCACCTGCG CGTGTCTCTC 
TTCGATGTCA CCTATCGGCA CTTCTTCGGG AGGACGTGGA GGAGCTCGCC GAGGCCTGCA 
GAGCCGCTCA CCAGGCAGCC GGCCAGGATC GTCTTCAATG AGCCCTTGTA TTTTCACACG 
TCCTTGAACC ACCCTCATAT TGTGGCCGTG GTGGAGGTGG TCGTTGAAGG CAGAAGACGA 
GACGGGACCC TCCAGACTCT GTCCTGTGGG TTTGGAATTC TTCGGATCTT CAGCCACAAG 
CCGGAATCTC CAACTTCCAC TTCCCAGGAC AAACGGCTGA GACTGTATCA TGGGAGCCCG 
CGAGCCCTCC TCCACCCGCT CCTTCAGGAC CCCATAGAAC AGAACAAGCA CATGACCCTC 
ATTGAGAACT GCAGCCTGCA GTACACGCTG AGGCCACACC CGCTCCTGGA GCCCGTGTTC 
CACCTTCTTC CCGAGAACTT GCTGGTGTCC GGTCTGCAGC AGATACCTGG CCTCCTTCCA 
GCTCACGGGG AATCAGGAGA TGCCCTCCGG AAGCCCCGCC TGCAGAAGCC TCTGACGTGC 
TACCTGGATG ATCTGTTCTT CACGCTGTAT CCCTCGCTGG AGAAGTTCGA GGAGGAGCTT 
CTGGAACTCG TGATCAGCGA TTGCTTCCGG GAGGCGGGCA GCCCGCTGGA CGTCGGCACC 
CTGGAGATCC TGGAGCGGCG CCTGTGCGTG GGCGTGCACA ATGGGTTGGG CTTCGTGCAG 
CGGCCGCAGG TCCTCGTGCT GGTGCCCGAG CTGGAGGTGG CCTTGACACG CTCTGCCAGC 
TTCAGCCGGA AGGTCGGCTC CTCTTCCAAG GCCAGCTCTG GAAACCAGGC GCTGGTCTTG 
AGAAGCCGTC TCCGACTCCC TGAGATGGTC CGTCACCCCG CGTTCGCCAT TGTCTTCCAG 
CTGGAGTACG TGTTTAACAG CCCCTCGGGG GCAGACAGCA ATGCAGCTTC ACTCTCCTCC 
TTGTCCAGCC TGGCATGTAT GCACATGGTT CGCTGGGCTG TCTGGAACCC CTCGCTGGAA 
GCCAGCTCCG GGCAGGTGAT GTTACCTCTC CAGGGTGGCA TCCAGTCCAA CCCTTCGCAC 
TGTCTGGTCT ATAAGGTACC CCCAGCCAGC ATAAGCTCCG AAGAGGTGAA GCAGGTGGAG 
TCAGGGACGG TCCAGTTCCA GTACTCGCTG AGCCCTGAAG AACACCCAGG CACACCCACA 
GCGAATGCAA GCAGCCCCAA GGCAGAGCGG CGGTCTTTTA GGAAGCCACC CGTGTCCCCT 
TCAAGTCCAC CAGCATCGCC GTCCCAGGGG CTCACCGCTG CCCAGGACTC ACCCATGGGA 
CCAGGGCTGT CACTTTCTCA GCTGGCGGCT TCCCCACCAT CCCCAGCTCC CCGCCGCCGC 
TCGGCCAAAC CTTCGTCACA GCCACCTGGG AGCCCTGTGC CCTCCCTGGT CCCAGGGGAG 
TTCCCCTTGG AGGGCAGGAT CTCTCACCTG GAGGCTGACC TGAGCCAGAC AGCCTTAGCC 
CTGGGAACGT CCGCTGCTGA CCACTTGCAG GAACTGCCTT TCACACCCGT GCACGCCCCG 
ATCATTGCAG GAACCCAGAC CAGGAGCTCT GGAGGGAAGC TGTCACGGGC ATCCCTGGCG 
CTGCTGCAGT CCGCTGGCTT TCCCGAGATT CTGGACGCCA ACAAGCAACC AGCTGAGGCT 
GTGAGTCCTA CAGACCCCGT GAAGTTCAGC CCTCAGAAGG AAGAATCAGA CTGTCTACAA 
GGCAATGAGA TAGTGTTGCA ATTTCTTGCC TTTAGCAGGG TGCCCCAGGA TGAGCAGGCA 
GCGCCATGGC CACAGACTGT GTACTTCACC TTCCAATTCT ACCGCTTCCC ACCCGAGACC 
ACGCCACGGC TGCAGCTCGT GGAGCTGGCC GATGCTGGGA AGACCAGCTC TGCCTCCCTG 
TCGCACATCC TCGCCCAGAT CCAGAAGGAT GGCTCCTTTG ATGCGGGGTC TCCGGGCTTT 
CAGCGGAAGT ACCTGGTGGA CCCCGGGTTC CTGAGACCCG GGGAGCAGCG CTGGTTCGCC 
CGCTACCTGG CCATGCAGAC CCTGCAGATC GACGTCTGGG ATGGGGACTC CCTGCTTCTC 
ATTGGATCTG CTGCCGTTCA GCTGAAGCAC CTTCTCCGCC AGGGGCGGCC GGCCGTGCAG 
GTCTCGCATG AGCTGGAGGT TGTGGCGACT GAATACGAGC AGGACGCCAT GGTGGTGAGT 
GGAGACATGA CCAAGTTTGG CTGCGTCAGA CCCATCGACG TCCACACGGT GGTGAAGGGC 
CAGCTGCACT TGACCTTGGC CAACGTGGGT CACTTGTGTG AACAGAGAGT AAGGAGTCCC 
AGTTCCCTGC CACCTTCCAG GTCGAGGGTC ATCTCAAATG ATGGAGTTGG CAACTTCATG 
GGAGGCAGCC TCCTGACACC CGGGGGCCCC AGATTGAAAA ATGTGGTCCA AGCTCAGAAG 
CTGGCTGATG TGGACAGCGA ACTGGCCGCC CTGCTCTTCA CACAACCGAG ACCAGGGGCC 
CGGGGGCCCC AGGGCATCAA TCAAGAGGGG GATGCCGTCC GCAGGCGCAA GCTGGAGCGC 
ATGAGGGCCG TGCGGCTGCG GGAATGTGGA TTGGAGCCGG GCCGCCGCGG GCTGGGCGCG 
CTGGCCCAGC AGAGCCTCCA TGCCCAGCAC GCCCGAGACC TGCAGGTCAT TGCCGCCTAC 
CGTGAGCGCA CAAAGGCGGA GAGCATCGCC AGCGCACTGA GCACGGCCAT CACCACGCAG 
CACACCATCC ACGCCACCCT AGGCGCTGCC GAGTTCTTCG AGTTCGCCCT GAGGAACCCC 
CACAACGCGC AGCACATGGT GACCATCGAG ATAGACAACC CCGAGCTCAG TCTCATCCTG 
GATGGTCAGG AGTGGCGGTA CTTCAAGGAC ATGGCTGACT TGCACACACC CCTGGAGGAG 
GACATGTTCC ACCTGCGGGG CAGCCTGGCC CCCCAACTCT TCCTGCGACC TCGGGAGACT 
GCCCACATCC CCTTCAAGTT CCAGACTTTC TCTGTGGGCC CCTGGGCCTC GGCACAGGCC 
TCTGCTGAGC TGAGATCAGA GAAGGACATG GATGCCGGAT CACCCTATAA GTCCAGCATG 
ATGCCCACCA AACACGCCAA GGTCCTGTTC CGCGCGGGCA GCGGCAAGCC CCTGGCCGTG 
CTCTGCCTGA CGGTGGAGCT GCAGCCTCAC GTGGTGGACC AGGTCTTCCG CTTCCACCAC 
CCGGAGCTCA CCTTCCTGAA GAAAGCCATC CGCCTGCCAC CCTGGCACAC GCTTCCAGGT 
GCCCCTGTAG GGACTTCCAG TGAGGACCCC CCTTTCCATG TTCGATGTAG TGACCCGAAC 
ATCATTTGTG AGACACAGAG TGTGGGCCCC GGGGAGCCCC GTGACGTGTT CCTGAAGGTG 
GCCAGTGGCC CTAGCCCAGA GGTCAAGGAC TTCTTTGTCA CCATCTACGT AGACCGCTGG 
TTAGCAACAC CTGTCCAGAC CTGGCAGGTC CACCTGCACT CGCTGCAGCG AGTGGACGTC 
TCCTGTATCA CTGGCCAGAG GACCCGCCTG GCTCTCGTCC TCCGGGGGAC GCAGACAGTT 
AGGAGAGTGC AGGCTTTCAG CTCACACCCC CAGGAGCTGA CGACGGACCC CAGAGGTGTG 
TTCGTGCTGC CTCCTCACGG GGTGCAGGAC TTGCATGTGA GCGTCAGGCC CTGCAGGCCG 
GGCAGCCGCT TCGCGCACCT CCACCTGGTT GACGTGGACG CCCACCAGCT GGTGGCCTCG 
TGGCTCCTGT GTCTCTCCTG CCGGCCGCCT CTCATCTCCA AGGCCTTCGA GATAAAGCTG 
CCCGCGGGGA GCGGGAGGGG CGCCAATAAG CGCATCACCT ACACCAATCC CTACCCCTCG 
CGGAGGGCCT ACCGCCTGCA CAGCGACCAC CCCCACCTGC TGCAGTTCAA GGAGGACGCC 
TTCCAGGTCG GGGGAGGTGA GACCTATACC ATCGGCCTGC GGTTTGTGTC CCGTCAGAGA 
GCCGGGGAGG AAGAGATCCT GATTTACATC AACGACCACG AAGACAAAAA CGAGGAGACC 
TTTTGCGTGA AGGTCATCTA CCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015322327.1
CDS	581..5107
Translation	MIVPSPPVPSRVSITAPCTLLSSVIFIGHNHLFCVLTCILCFCLPSDTFLEVIVVVCSVLQGLAQGNPRGPRDPGTSRMHDWHRIFDQNLLVPPHPQRARQPLKESTAFQCVLKWLDGPLIKQGVLELLSEVGCHLRVSLFDVTYRHFFGRTWRSSPRPAEPLTRQPARIVFNEPLYFHTSLNHPHIVAVVEVVVEGRRRDGTLQTLSCGFGILRIFSHKPESPTSTSQDKRLRLYHGSPRALLHPLLQDPIEQNKHMTLIENCSLQYTLRPHPLLEPVFHLLPENLLVSGLQQIPGLLPAHGESGDALRKPRLQKPLTCYLDDLFFTLYPSLEKFEEELLELVISDCFREAGSPLDVGTLEILERRLCVGVHNGLGFVQRPQVLVLVPELEVALTRSASFSRKVGSSSKASSGNQALVLRSRLRLPEMVRHPAFAIVFQLEYVFNSPSGADSNAASLSSLSSLACMHMVRWAVWNPSLEASSGQVMLPLQGGIQSNPSHCLVYKVPPASISSEEVKQVESGTVQFQYSLSPEEHPGTPTANASSPKAERRSFRKPPVSPSSPPASPSQGLTAAQDSPMGPGLSLSQLAASPPSPAPRRRSAKPSSQPPGSPVPSLVPGEFPLEGRISHLEADLSQTALALGTSAADHLQELPFTPVHAPIIAGTQTRSSGGKLSRASLALLQSAGFPEILDANKQPAEAVSPTDPVKFSPQKEESDCLQGNEIVLQFLAFSRVPQDEQAAPWPQTVYFTFQFYRFPPETTPRLQLVELADAGKTSSASLSHILAQIQKDGSFDAGSPGFQRKYLVDPGFLRPGEQRWFARYLAMQTLQIDVWDGDSLLLIGSAAVQLKHLLRQGRPAVQVSHELEVVATEYEQDAMVVSGDMTKFGCVRPIDVHTVVKGQLHLTLANVGHLCEQRVRSPSSLPPSRSRVISNDGVGNFMGGSLLTPGGPRLKNVVQAQKLADVDSELAALLFTQPRPGARGPQGINQEGDAVRRRKLERMRAVRLRECGLEPGRRGLGALAQQSLHAQHARDLQVIAAYRERTKAESIASALSTAITTQHTIHATLGAAEFFEFALRNPHNAQHMVTIEIDNPELSLILDGQEWRYFKDMADLHTPLEEDMFHLRGSLAPQLFLRPRETAHIPFKFQTFSVGPWASAQASAELRSEKDMDAGSPYKSSMMPTKHAKVLFRAGSGKPLAVLCLTVELQPHVVDQVFRFHHPELTFLKKAIRLPPWHTLPGAPVGTSSEDPPFHVRCSDPNIICETQSVGPGEPRDVFLKVASGPSPEVKDFFVTIYVDRWLATPVQTWQVHLHSLQRVDVSCITGQRTRLALVLRGTQTVRRVQAFSSHPQELTTDPRGVFVLPPHGVQDLHVSVRPCRPGSRFAHLHLVDVDAHQLVASWLLCLSCRPPLISKAFEIKLPAGSGRGANKRITYTNPYPSRRAYRLHSDHPHLLQFKEDAFQVGGGETYTIGLRFVSRQRAGEEEILIYINDHEDKNEETFCVKVIYQ

Target ORF information:

RefSeq Version	XM_015466841.1
Organism	Bos taurus(cattle)
Definition	Bos taurus nephronophthisis 4 (NPHP4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015466841.1

ORF Insert Sequence:

ATGATTGTGC CGTCGCCGCC AGTTCCAAGC CGGGTCTCCA TCACAGCTCC CTGCACCCTC 
CTCTCCTCTG TGATTTTCAT AGGTCACAAC CACCTCTTTT GTGTGCTAAC TTGTATCCTG 
TGTTTCTGCC TCCCTTCAGA TACATTCCTT GAGGTCATCG TGGTTGTCTG CAGTGTTCTC 
CAGGGCCTGG CACAGGGTAA TCCCAGAGGC CCCAGGGACC CGGGGACCAG CAGAATGCAC 
GACTGGCACA GGATCTTTGA CCAAAACCTG CTGGTCCCTC CGCACCCACA GAGAGCACGC 
CAGCCTCTGA AGGAATCGAC GGCGTTTCAG TGTGTCCTGA AGTGGCTTGA TGGCCCGCTA 
ATTAAGCAGG GGGTGCTGGA GCTGCTATCG GAGGTGGGCT GCCACCTGCG CGTGTCTCTC 
TTCGATGTCA CCTATCGGCA CTTCTTCGGG AGGACGTGGA GGAGCTCGCC GAGGCCTGCA 
GAGCCGCTCA CCAGGCAGCC GGCCAGGATC GTCTTCAATG AGCCCTTGTA TTTTCACACG 
TCCTTGAACC ACCCTCATAT TGTGGCCGTG GTGGAGGTGG TCGTTGAAGG CAGAAGACGA 
GACGGGACCC TCCAGACTCT GTCCTGTGGG TTTGGAATTC TTCGGATCTT CAGCCACAAG 
CCGGAATCTC CAACTTCCAC TTCCCAGGAC AAACGGCTGA GACTGTATCA TGGGAGCCCG 
CGAGCCCTCC TCCACCCGCT CCTTCAGGAC CCCATAGAAC AGAACAAGCA CATGACCCTC 
ATTGAGAACT GCAGCCTGCA GTACACGCTG AGGCCACACC CGCTCCTGGA GCCCGTGTTC 
CACCTTCTTC CCGAGAACTT GCTGGTGTCC GGTCTGCAGC AGATACCTGG CCTCCTTCCA 
GCTCACGGGG AATCAGGAGA TGCCCTCCGG AAGCCCCGCC TGCAGAAGCC TCTGACGTGC 
TACCTGGATG ATCTGTTCTT CACGCTGTAT CCCTCGCTGG AGAAGTTCGA GGAGGAGCTT 
CTGGAACTCG TGATCAGCGA TTGCTTCCGG GAGGCGGGCA GCCCGCTGGA CGTCGGCACC 
CTGGAGATCC TGGAGCGGCG CCTGTGCGTG GGCGTGCACA ATGGGTTGGG CTTCGTGCAG 
CGGCCGCAGG TCCTCGTGCT GGTGCCCGAG CTGGAGGTGG CCTTGACACG CTCTGCCAGC 
TTCAGCCGGA AGGTCGGCTC CTCTTCCAAG GCCAGCTCTG GAAACCAGGC GCTGGTCTTG 
AGAAGCCGTC TCCGACTCCC TGAGATGGTC CGTCACCCCG CGTTCGCCAT TGTCTTCCAG 
CTGGAGTACG TGTTTAACAG CCCCTCGGGG GCAGACAGCA ATGCAGCTTC ACTCTCCTCC 
TTGTCCAGCC TGGCATGTAT GCACATGGTT CGCTGGGCTG TCTGGAACCC CTCGCTGGAA 
GCCAGCTCCG GGCAGGTGAT GTTACCTCTC CAGGGTGGCA TCCAGTCCAA CCCTTCGCAC 
TGTCTGGTCT ATAAGGTACC CCCAGCCAGC ATAAGCTCCG AAGAGGTGAA GCAGGTGGAG 
TCAGGGACGG TCCAGTTCCA GTACTCGCTG AGCCCTGAAG AACACCCAGG CACACCCACA 
GCGAATGCAA GCAGCCCCAA GGCAGAGCGG CGGTCTTTTA GGAAGCCACC CGTGTCCCCT 
TCAAGTCCAC CAGCATCGCC GTCCCAGGGG CTCACCGCTG CCCAGGACTC ACCCATGGGA 
CCAGGGCTGT CACTTTCTCA GCTGGCGGCT TCCCCACCAT CCCCAGCTCC CCGCCGCCGC 
TCGGCCAAAC CTTCGTCACA GCCACCTGGG AGCCCTGTGC CCTCCCTGGT CCCAGGGGAG 
TTCCCCTTGG AGGGCAGGAT CTCTCACCTG GAGGCTGACC TGAGCCAGAC AGCCTTAGCC 
CTGGGAACGT CCGCTGCTGA CCACTTGCAG GAACTGCCTT TCACACCCGT GCACGCCCCG 
ATCATTGCAG GAACCCAGAC CAGGAGCTCT GGAGGGAAGC TGTCACGGGC ATCCCTGGCG 
CTGCTGCAGT CCGCTGGCTT TCCCGAGATT CTGGACGCCA ACAAGCAACC AGCTGAGGCT 
GTGAGTCCTA CAGACCCCGT GAAGTTCAGC CCTCAGAAGG AAGAATCAGA CTGTCTACAA 
GGCAATGAGA TAGTGTTGCA ATTTCTTGCC TTTAGCAGGG TGCCCCAGGA TGAGCAGGCA 
GCGCCATGGC CACAGACTGT GTACTTCACC TTCCAATTCT ACCGCTTCCC ACCCGAGACC 
ACGCCACGGC TGCAGCTCGT GGAGCTGGCC GATGCTGGGA AGACCAGCTC TGCCTCCCTG 
TCGCACATCC TCGCCCAGAT CCAGAAGGAT GGCTCCTTTG ATGCGGGGTC TCCGGGCTTT 
CAGCGGAAGT ACCTGGTGGA CCCCGGGTTC CTGAGACCCG GGGAGCAGCG CTGGTTCGCC 
CGCTACCTGG CCATGCAGAC CCTGCAGATC GACGTCTGGG ATGGGGACTC CCTGCTTCTC 
ATTGGATCTG CTGCCGTTCA GCTGAAGCAC CTTCTCCGCC AGGGGCGGCC GGCCGTGCAG 
GTCTCGCATG AGCTGGAGGT TGTGGCGACT GAATACGAGC AGGACGCCAT GGTGGTGAGT 
GGAGACATGA CCAAGTTTGG CTGCGTCAGA CCCATCGACG TCCACACGGT GGTGAAGGGC 
CAGCTGCACT TGACCTTGGC CAACGTGGGT CACTTGTGTG AACAGAGAGT AAGGAGTCCC 
AGTTCCCTGC CACCTTCCAG GTCGAGGGTC ATCTCAAATG ATGGAGTTGG CAACTTCATG 
GGAGGCAGCC TCCTGACACC CGGGGGCCCC AGATTGAAAA ATGTGGTCCA AGCTCAGAAG 
CTGGCTGATG TGGACAGCGA ACTGGCCGCC CTGCTCTTCA CACAACCGAG ACCAGGGGCC 
CGGGGGCCCC AGGGCATCAA TCAAGAGGGG GATGCCGTCC GCAGGCGCAA GCTGGAGCGC 
ATGAGGGCCG TGCGGCTGCG GGAATGTGGA TTGGAGCCGG GCCGCCGCGG GCTGGGCGCG 
CTGGCCCAGC AGAGCCTCCA TGCCCAGCAC GCCCGAGACC TGCAGGTCAT TGCCGCCTAC 
CGTGAGCGCA CAAAGGCGGA GAGCATCGCC AGCGCACTGA GCACGGCCAT CACCACGCAG 
CACACCATCC ACGCCACCCT AGGCGCTGCC GAGTTCTTCG AGTTCGCCCT GAGGAACCCC 
CACAACGCGC AGCACATGGT GACCATCGAG ATAGACAACC CCGAGCTCAG TCTCATCCTG 
GATGGTCAGG AGTGGCGGTA CTTCAAGGAC ATGGCTGACT TGCACACACC CCTGGAGGAG 
GACATGTTCC ACCTGCGGGG CAGCCTGGCC CCCCAACTCT TCCTGCGACC TCGGGAGACT 
GCCCACATCC CCTTCAAGTT CCAGACTTTC TCTGTGGGCC CCTGGGCCTC GGCACAGGCC 
TCTGCTGAGC TGAGATCAGA GAAGGACATG GATGCCGGAT CACCCTATAA GTCCAGCATG 
ATGCCCACCA AACACGCCAA GGTCCTGTTC CGCGCGGGCA GCGGCAAGCC CCTGGCCGTG 
CTCTGCCTGA CGGTGGAGCT GCAGCCTCAC GTGGTGGACC AGGTCTTCCG CTTCCACCAC 
CCGGAGCTCA CCTTCCTGAA GAAAGCCATC CGCCTGCCAC CCTGGCACAC GCTTCCAGGT 
GCCCCTGTAG GGACTTCCAG TGAGGACCCC CCTTTCCATG TTCGATGTAG TGACCCGAAC 
ATCATTTGTG AGACACAGAG TGTGGGCCCC GGGGAGCCCC GTGACGTGTT CCTGAAGGTG 
GCCAGTGGCC CTAGCCCAGA GGTCAAGGAC TTCTTTGTCA CCATCTACGT AGACCGCTGG 
TTAGCAACAC CTGTCCAGAC CTGGCAGGTC CACCTGCACT CGCTGCAGCG AGTGGACGTC 
TCCTGTATCA CTGGCCAGAG GACCCGCCTG GCTCTCGTCC TCCGGGGGAC GCAGACAGTT 
AGGAGAGTGC AGGCTTTCAG CTCACACCCC CAGGAGCTGA CGACGGACCC CAGAGGTGTG 
TTCGTGCTGC CTCCTCACGG GGTGCAGGAC TTGCATGTGA GCGTCAGGCC CTGCAGGCCG 
GGCAGCCGCT TCGCGCACCT CCACCTGGTT GACGTGGACG CCCACCAGCT GGTGGCCTCG 
TGGCTCCTGT GTCTCTCCTG CCGGCCGCCT CTCATCTCCA AGGCCTTCGA GATAAAGCTG 
CCCGCGGGGA GCGGGAGGGG CGCCAATAAG CGCATCACCT ACACCAATCC CTACCCCTCG 
CGGAGGGCCT ACCGCCTGCA CAGCGACCAC CCCCACCTGC TGCAGTTCAA GGAGGACGCC 
TTCCAGGTCG GGGGAGGTGA GACCTATACC ATCGGCCTGC GGTTTGTGTC CCGTCAGAGA 
GCCGGGGAGG AAGAGATCCT GATTTACATC AACGACCACG AAGACAAAAA CGAGGAGACC 
TTTTGCGTGA AGGTCATCTA CCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPHP4 ( XM_010813384.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010811686.1 Bos taurus nephronophthisis 4 (NPHP4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa50068
Clone ID Related Accession (Same CDS sequence)	XM_015466841.1 , XM_010813384.2
Accession Version	XM_010813384.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4527bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	nephrocystin-4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104439.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010813384.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGTGC CGTCGCCGCC AGTTCCAAGC CGGGTCTCCA TCACAGCTCC CTGCACCCTC 
CTCTCCTCTG TGATTTTCAT AGGTCACAAC CACCTCTTTT GTGTGCTAAC TTGTATCCTG 
TGTTTCTGCC TCCCTTCAGA TACATTCCTT GAGGTCATCG TGGTTGTCTG CAGTGTTCTC 
CAGGGCCTGG CACAGGGTAA TCCCAGAGGC CCCAGGGACC CGGGGACCAG CAGAATGCAC 
GACTGGCACA GGATCTTTGA CCAAAACCTG CTGGTCCCTC CGCACCCACA GAGAGCACGC 
CAGCCTCTGA AGGAATCGAC GGCGTTTCAG TGTGTCCTGA AGTGGCTTGA TGGCCCGCTA 
ATTAAGCAGG GGGTGCTGGA GCTGCTATCG GAGGTGGGCT GCCACCTGCG CGTGTCTCTC 
TTCGATGTCA CCTATCGGCA CTTCTTCGGG AGGACGTGGA GGAGCTCGCC GAGGCCTGCA 
GAGCCGCTCA CCAGGCAGCC GGCCAGGATC GTCTTCAATG AGCCCTTGTA TTTTCACACG 
TCCTTGAACC ACCCTCATAT TGTGGCCGTG GTGGAGGTGG TCGTTGAAGG CAGAAGACGA 
GACGGGACCC TCCAGACTCT GTCCTGTGGG TTTGGAATTC TTCGGATCTT CAGCCACAAG 
CCGGAATCTC CAACTTCCAC TTCCCAGGAC AAACGGCTGA GACTGTATCA TGGGAGCCCG 
CGAGCCCTCC TCCACCCGCT CCTTCAGGAC CCCATAGAAC AGAACAAGCA CATGACCCTC 
ATTGAGAACT GCAGCCTGCA GTACACGCTG AGGCCACACC CGCTCCTGGA GCCCGTGTTC 
CACCTTCTTC CCGAGAACTT GCTGGTGTCC GGTCTGCAGC AGATACCTGG CCTCCTTCCA 
GCTCACGGGG AATCAGGAGA TGCCCTCCGG AAGCCCCGCC TGCAGAAGCC TCTGACGTGC 
TACCTGGATG ATCTGTTCTT CACGCTGTAT CCCTCGCTGG AGAAGTTCGA GGAGGAGCTT 
CTGGAACTCG TGATCAGCGA TTGCTTCCGG GAGGCGGGCA GCCCGCTGGA CGTCGGCACC 
CTGGAGATCC TGGAGCGGCG CCTGTGCGTG GGCGTGCACA ATGGGTTGGG CTTCGTGCAG 
CGGCCGCAGG TCCTCGTGCT GGTGCCCGAG CTGGAGGTGG CCTTGACACG CTCTGCCAGC 
TTCAGCCGGA AGGTCGGCTC CTCTTCCAAG GCCAGCTCTG GAAACCAGGC GCTGGTCTTG 
AGAAGCCGTC TCCGACTCCC TGAGATGGTC CGTCACCCCG CGTTCGCCAT TGTCTTCCAG 
CTGGAGTACG TGTTTAACAG CCCCTCGGGG GCAGACAGCA ATGCAGCTTC ACTCTCCTCC 
TTGTCCAGCC TGGCATGTAT GCACATGGTT CGCTGGGCTG TCTGGAACCC CTCGCTGGAA 
GCCAGCTCCG GGCAGGTGAT GTTACCTCTC CAGGGTGGCA TCCAGTCCAA CCCTTCGCAC 
TGTCTGGTCT ATAAGGTACC CCCAGCCAGC ATAAGCTCCG AAGAGGTGAA GCAGGTGGAG 
TCAGGGACGG TCCAGTTCCA GTACTCGCTG AGCCCTGAAG AACACCCAGG CACACCCACA 
GCGAATGCAA GCAGCCCCAA GGCAGAGCGG CGGTCTTTTA GGAAGCCACC CGTGTCCCCT 
TCAAGTCCAC CAGCATCGCC GTCCCAGGGG CTCACCGCTG CCCAGGACTC ACCCATGGGA 
CCAGGGCTGT CACTTTCTCA GCTGGCGGCT TCCCCACCAT CCCCAGCTCC CCGCCGCCGC 
TCGGCCAAAC CTTCGTCACA GCCACCTGGG AGCCCTGTGC CCTCCCTGGT CCCAGGGGAG 
TTCCCCTTGG AGGGCAGGAT CTCTCACCTG GAGGCTGACC TGAGCCAGAC AGCCTTAGCC 
CTGGGAACGT CCGCTGCTGA CCACTTGCAG GAACTGCCTT TCACACCCGT GCACGCCCCG 
ATCATTGCAG GAACCCAGAC CAGGAGCTCT GGAGGGAAGC TGTCACGGGC ATCCCTGGCG 
CTGCTGCAGT CCGCTGGCTT TCCCGAGATT CTGGACGCCA ACAAGCAACC AGCTGAGGCT 
GTGAGTCCTA CAGACCCCGT GAAGTTCAGC CCTCAGAAGG AAGAATCAGA CTGTCTACAA 
GGCAATGAGA TAGTGTTGCA ATTTCTTGCC TTTAGCAGGG TGCCCCAGGA TGAGCAGGCA 
GCGCCATGGC CACAGACTGT GTACTTCACC TTCCAATTCT ACCGCTTCCC ACCCGAGACC 
ACGCCACGGC TGCAGCTCGT GGAGCTGGCC GATGCTGGGA AGACCAGCTC TGCCTCCCTG 
TCGCACATCC TCGCCCAGAT CCAGAAGGAT GGCTCCTTTG ATGCGGGGTC TCCGGGCTTT 
CAGCGGAAGT ACCTGGTGGA CCCCGGGTTC CTGAGACCCG GGGAGCAGCG CTGGTTCGCC 
CGCTACCTGG CCATGCAGAC CCTGCAGATC GACGTCTGGG ATGGGGACTC CCTGCTTCTC 
ATTGGATCTG CTGCCGTTCA GCTGAAGCAC CTTCTCCGCC AGGGGCGGCC GGCCGTGCAG 
GTCTCGCATG AGCTGGAGGT TGTGGCGACT GAATACGAGC AGGACGCCAT GGTGGTGAGT 
GGAGACATGA CCAAGTTTGG CTGCGTCAGA CCCATCGACG TCCACACGGT GGTGAAGGGC 
CAGCTGCACT TGACCTTGGC CAACGTGGGT CACTTGTGTG AACAGAGAGT AAGGAGTCCC 
AGTTCCCTGC CACCTTCCAG GTCGAGGGTC ATCTCAAATG ATGGAGTTGG CAACTTCATG 
GGAGGCAGCC TCCTGACACC CGGGGGCCCC AGATTGAAAA ATGTGGTCCA AGCTCAGAAG 
CTGGCTGATG TGGACAGCGA ACTGGCCGCC CTGCTCTTCA CACAACCGAG ACCAGGGGCC 
CGGGGGCCCC AGGGCATCAA TCAAGAGGGG GATGCCGTCC GCAGGCGCAA GCTGGAGCGC 
ATGAGGGCCG TGCGGCTGCG GGAATGTGGA TTGGAGCCGG GCCGCCGCGG GCTGGGCGCG 
CTGGCCCAGC AGAGCCTCCA TGCCCAGCAC GCCCGAGACC TGCAGGTCAT TGCCGCCTAC 
CGTGAGCGCA CAAAGGCGGA GAGCATCGCC AGCGCACTGA GCACGGCCAT CACCACGCAG 
CACACCATCC ACGCCACCCT AGGCGCTGCC GAGTTCTTCG AGTTCGCCCT GAGGAACCCC 
CACAACGCGC AGCACATGGT GACCATCGAG ATAGACAACC CCGAGCTCAG TCTCATCCTG 
GATGGTCAGG AGTGGCGGTA CTTCAAGGAC ATGGCTGACT TGCACACACC CCTGGAGGAG 
GACATGTTCC ACCTGCGGGG CAGCCTGGCC CCCCAACTCT TCCTGCGACC TCGGGAGACT 
GCCCACATCC CCTTCAAGTT CCAGACTTTC TCTGTGGGCC CCTGGGCCTC GGCACAGGCC 
TCTGCTGAGC TGAGATCAGA GAAGGACATG GATGCCGGAT CACCCTATAA GTCCAGCATG 
ATGCCCACCA AACACGCCAA GGTCCTGTTC CGCGCGGGCA GCGGCAAGCC CCTGGCCGTG 
CTCTGCCTGA CGGTGGAGCT GCAGCCTCAC GTGGTGGACC AGGTCTTCCG CTTCCACCAC 
CCGGAGCTCA CCTTCCTGAA GAAAGCCATC CGCCTGCCAC CCTGGCACAC GCTTCCAGGT 
GCCCCTGTAG GGACTTCCAG TGAGGACCCC CCTTTCCATG TTCGATGTAG TGACCCGAAC 
ATCATTTGTG AGACACAGAG TGTGGGCCCC GGGGAGCCCC GTGACGTGTT CCTGAAGGTG 
GCCAGTGGCC CTAGCCCAGA GGTCAAGGAC TTCTTTGTCA CCATCTACGT AGACCGCTGG 
TTAGCAACAC CTGTCCAGAC CTGGCAGGTC CACCTGCACT CGCTGCAGCG AGTGGACGTC 
TCCTGTATCA CTGGCCAGAG GACCCGCCTG GCTCTCGTCC TCCGGGGGAC GCAGACAGTT 
AGGAGAGTGC AGGCTTTCAG CTCACACCCC CAGGAGCTGA CGACGGACCC CAGAGGTGTG 
TTCGTGCTGC CTCCTCACGG GGTGCAGGAC TTGCATGTGA GCGTCAGGCC CTGCAGGCCG 
GGCAGCCGCT TCGCGCACCT CCACCTGGTT GACGTGGACG CCCACCAGCT GGTGGCCTCG 
TGGCTCCTGT GTCTCTCCTG CCGGCCGCCT CTCATCTCCA AGGCCTTCGA GATAAAGCTG 
CCCGCGGGGA GCGGGAGGGG CGCCAATAAG CGCATCACCT ACACCAATCC CTACCCCTCG 
CGGAGGGCCT ACCGCCTGCA CAGCGACCAC CCCCACCTGC TGCAGTTCAA GGAGGACGCC 
TTCCAGGTCG GGGGAGGTGA GACCTATACC ATCGGCCTGC GGTTTGTGTC CCGTCAGAGA 
GCCGGGGAGG AAGAGATCCT GATTTACATC AACGACCACG AAGACAAAAA CGAGGAGACC 
TTTTGCGTGA AGGTCATCTA CCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010811686.1
CDS	581..5107
Translation	MIVPSPPVPSRVSITAPCTLLSSVIFIGHNHLFCVLTCILCFCLPSDTFLEVIVVVCSVLQGLAQGNPRGPRDPGTSRMHDWHRIFDQNLLVPPHPQRARQPLKESTAFQCVLKWLDGPLIKQGVLELLSEVGCHLRVSLFDVTYRHFFGRTWRSSPRPAEPLTRQPARIVFNEPLYFHTSLNHPHIVAVVEVVVEGRRRDGTLQTLSCGFGILRIFSHKPESPTSTSQDKRLRLYHGSPRALLHPLLQDPIEQNKHMTLIENCSLQYTLRPHPLLEPVFHLLPENLLVSGLQQIPGLLPAHGESGDALRKPRLQKPLTCYLDDLFFTLYPSLEKFEEELLELVISDCFREAGSPLDVGTLEILERRLCVGVHNGLGFVQRPQVLVLVPELEVALTRSASFSRKVGSSSKASSGNQALVLRSRLRLPEMVRHPAFAIVFQLEYVFNSPSGADSNAASLSSLSSLACMHMVRWAVWNPSLEASSGQVMLPLQGGIQSNPSHCLVYKVPPASISSEEVKQVESGTVQFQYSLSPEEHPGTPTANASSPKAERRSFRKPPVSPSSPPASPSQGLTAAQDSPMGPGLSLSQLAASPPSPAPRRRSAKPSSQPPGSPVPSLVPGEFPLEGRISHLEADLSQTALALGTSAADHLQELPFTPVHAPIIAGTQTRSSGGKLSRASLALLQSAGFPEILDANKQPAEAVSPTDPVKFSPQKEESDCLQGNEIVLQFLAFSRVPQDEQAAPWPQTVYFTFQFYRFPPETTPRLQLVELADAGKTSSASLSHILAQIQKDGSFDAGSPGFQRKYLVDPGFLRPGEQRWFARYLAMQTLQIDVWDGDSLLLIGSAAVQLKHLLRQGRPAVQVSHELEVVATEYEQDAMVVSGDMTKFGCVRPIDVHTVVKGQLHLTLANVGHLCEQRVRSPSSLPPSRSRVISNDGVGNFMGGSLLTPGGPRLKNVVQAQKLADVDSELAALLFTQPRPGARGPQGINQEGDAVRRRKLERMRAVRLRECGLEPGRRGLGALAQQSLHAQHARDLQVIAAYRERTKAESIASALSTAITTQHTIHATLGAAEFFEFALRNPHNAQHMVTIEIDNPELSLILDGQEWRYFKDMADLHTPLEEDMFHLRGSLAPQLFLRPRETAHIPFKFQTFSVGPWASAQASAELRSEKDMDAGSPYKSSMMPTKHAKVLFRAGSGKPLAVLCLTVELQPHVVDQVFRFHHPELTFLKKAIRLPPWHTLPGAPVGTSSEDPPFHVRCSDPNIICETQSVGPGEPRDVFLKVASGPSPEVKDFFVTIYVDRWLATPVQTWQVHLHSLQRVDVSCITGQRTRLALVLRGTQTVRRVQAFSSHPQELTTDPRGVFVLPPHGVQDLHVSVRPCRPGSRFAHLHLVDVDAHQLVASWLLCLSCRPPLISKAFEIKLPAGSGRGANKRITYTNPYPSRRAYRLHSDHPHLLQFKEDAFQVGGGETYTIGLRFVSRQRAGEEEILIYINDHEDKNEETFCVKVIYQ

Target ORF information:

RefSeq Version	XM_010813384.2
Organism	Bos taurus(cattle)
Definition	Bos taurus nephronophthisis 4 (NPHP4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010813384.2

ORF Insert Sequence:

ATGATTGTGC CGTCGCCGCC AGTTCCAAGC CGGGTCTCCA TCACAGCTCC CTGCACCCTC 
CTCTCCTCTG TGATTTTCAT AGGTCACAAC CACCTCTTTT GTGTGCTAAC TTGTATCCTG 
TGTTTCTGCC TCCCTTCAGA TACATTCCTT GAGGTCATCG TGGTTGTCTG CAGTGTTCTC 
CAGGGCCTGG CACAGGGTAA TCCCAGAGGC CCCAGGGACC CGGGGACCAG CAGAATGCAC 
GACTGGCACA GGATCTTTGA CCAAAACCTG CTGGTCCCTC CGCACCCACA GAGAGCACGC 
CAGCCTCTGA AGGAATCGAC GGCGTTTCAG TGTGTCCTGA AGTGGCTTGA TGGCCCGCTA 
ATTAAGCAGG GGGTGCTGGA GCTGCTATCG GAGGTGGGCT GCCACCTGCG CGTGTCTCTC 
TTCGATGTCA CCTATCGGCA CTTCTTCGGG AGGACGTGGA GGAGCTCGCC GAGGCCTGCA 
GAGCCGCTCA CCAGGCAGCC GGCCAGGATC GTCTTCAATG AGCCCTTGTA TTTTCACACG 
TCCTTGAACC ACCCTCATAT TGTGGCCGTG GTGGAGGTGG TCGTTGAAGG CAGAAGACGA 
GACGGGACCC TCCAGACTCT GTCCTGTGGG TTTGGAATTC TTCGGATCTT CAGCCACAAG 
CCGGAATCTC CAACTTCCAC TTCCCAGGAC AAACGGCTGA GACTGTATCA TGGGAGCCCG 
CGAGCCCTCC TCCACCCGCT CCTTCAGGAC CCCATAGAAC AGAACAAGCA CATGACCCTC 
ATTGAGAACT GCAGCCTGCA GTACACGCTG AGGCCACACC CGCTCCTGGA GCCCGTGTTC 
CACCTTCTTC CCGAGAACTT GCTGGTGTCC GGTCTGCAGC AGATACCTGG CCTCCTTCCA 
GCTCACGGGG AATCAGGAGA TGCCCTCCGG AAGCCCCGCC TGCAGAAGCC TCTGACGTGC 
TACCTGGATG ATCTGTTCTT CACGCTGTAT CCCTCGCTGG AGAAGTTCGA GGAGGAGCTT 
CTGGAACTCG TGATCAGCGA TTGCTTCCGG GAGGCGGGCA GCCCGCTGGA CGTCGGCACC 
CTGGAGATCC TGGAGCGGCG CCTGTGCGTG GGCGTGCACA ATGGGTTGGG CTTCGTGCAG 
CGGCCGCAGG TCCTCGTGCT GGTGCCCGAG CTGGAGGTGG CCTTGACACG CTCTGCCAGC 
TTCAGCCGGA AGGTCGGCTC CTCTTCCAAG GCCAGCTCTG GAAACCAGGC GCTGGTCTTG 
AGAAGCCGTC TCCGACTCCC TGAGATGGTC CGTCACCCCG CGTTCGCCAT TGTCTTCCAG 
CTGGAGTACG TGTTTAACAG CCCCTCGGGG GCAGACAGCA ATGCAGCTTC ACTCTCCTCC 
TTGTCCAGCC TGGCATGTAT GCACATGGTT CGCTGGGCTG TCTGGAACCC CTCGCTGGAA 
GCCAGCTCCG GGCAGGTGAT GTTACCTCTC CAGGGTGGCA TCCAGTCCAA CCCTTCGCAC 
TGTCTGGTCT ATAAGGTACC CCCAGCCAGC ATAAGCTCCG AAGAGGTGAA GCAGGTGGAG 
TCAGGGACGG TCCAGTTCCA GTACTCGCTG AGCCCTGAAG AACACCCAGG CACACCCACA 
GCGAATGCAA GCAGCCCCAA GGCAGAGCGG CGGTCTTTTA GGAAGCCACC CGTGTCCCCT 
TCAAGTCCAC CAGCATCGCC GTCCCAGGGG CTCACCGCTG CCCAGGACTC ACCCATGGGA 
CCAGGGCTGT CACTTTCTCA GCTGGCGGCT TCCCCACCAT CCCCAGCTCC CCGCCGCCGC 
TCGGCCAAAC CTTCGTCACA GCCACCTGGG AGCCCTGTGC CCTCCCTGGT CCCAGGGGAG 
TTCCCCTTGG AGGGCAGGAT CTCTCACCTG GAGGCTGACC TGAGCCAGAC AGCCTTAGCC 
CTGGGAACGT CCGCTGCTGA CCACTTGCAG GAACTGCCTT TCACACCCGT GCACGCCCCG 
ATCATTGCAG GAACCCAGAC CAGGAGCTCT GGAGGGAAGC TGTCACGGGC ATCCCTGGCG 
CTGCTGCAGT CCGCTGGCTT TCCCGAGATT CTGGACGCCA ACAAGCAACC AGCTGAGGCT 
GTGAGTCCTA CAGACCCCGT GAAGTTCAGC CCTCAGAAGG AAGAATCAGA CTGTCTACAA 
GGCAATGAGA TAGTGTTGCA ATTTCTTGCC TTTAGCAGGG TGCCCCAGGA TGAGCAGGCA 
GCGCCATGGC CACAGACTGT GTACTTCACC TTCCAATTCT ACCGCTTCCC ACCCGAGACC 
ACGCCACGGC TGCAGCTCGT GGAGCTGGCC GATGCTGGGA AGACCAGCTC TGCCTCCCTG 
TCGCACATCC TCGCCCAGAT CCAGAAGGAT GGCTCCTTTG ATGCGGGGTC TCCGGGCTTT 
CAGCGGAAGT ACCTGGTGGA CCCCGGGTTC CTGAGACCCG GGGAGCAGCG CTGGTTCGCC 
CGCTACCTGG CCATGCAGAC CCTGCAGATC GACGTCTGGG ATGGGGACTC CCTGCTTCTC 
ATTGGATCTG CTGCCGTTCA GCTGAAGCAC CTTCTCCGCC AGGGGCGGCC GGCCGTGCAG 
GTCTCGCATG AGCTGGAGGT TGTGGCGACT GAATACGAGC AGGACGCCAT GGTGGTGAGT 
GGAGACATGA CCAAGTTTGG CTGCGTCAGA CCCATCGACG TCCACACGGT GGTGAAGGGC 
CAGCTGCACT TGACCTTGGC CAACGTGGGT CACTTGTGTG AACAGAGAGT AAGGAGTCCC 
AGTTCCCTGC CACCTTCCAG GTCGAGGGTC ATCTCAAATG ATGGAGTTGG CAACTTCATG 
GGAGGCAGCC TCCTGACACC CGGGGGCCCC AGATTGAAAA ATGTGGTCCA AGCTCAGAAG 
CTGGCTGATG TGGACAGCGA ACTGGCCGCC CTGCTCTTCA CACAACCGAG ACCAGGGGCC 
CGGGGGCCCC AGGGCATCAA TCAAGAGGGG GATGCCGTCC GCAGGCGCAA GCTGGAGCGC 
ATGAGGGCCG TGCGGCTGCG GGAATGTGGA TTGGAGCCGG GCCGCCGCGG GCTGGGCGCG 
CTGGCCCAGC AGAGCCTCCA TGCCCAGCAC GCCCGAGACC TGCAGGTCAT TGCCGCCTAC 
CGTGAGCGCA CAAAGGCGGA GAGCATCGCC AGCGCACTGA GCACGGCCAT CACCACGCAG 
CACACCATCC ACGCCACCCT AGGCGCTGCC GAGTTCTTCG AGTTCGCCCT GAGGAACCCC 
CACAACGCGC AGCACATGGT GACCATCGAG ATAGACAACC CCGAGCTCAG TCTCATCCTG 
GATGGTCAGG AGTGGCGGTA CTTCAAGGAC ATGGCTGACT TGCACACACC CCTGGAGGAG 
GACATGTTCC ACCTGCGGGG CAGCCTGGCC CCCCAACTCT TCCTGCGACC TCGGGAGACT 
GCCCACATCC CCTTCAAGTT CCAGACTTTC TCTGTGGGCC CCTGGGCCTC GGCACAGGCC 
TCTGCTGAGC TGAGATCAGA GAAGGACATG GATGCCGGAT CACCCTATAA GTCCAGCATG 
ATGCCCACCA AACACGCCAA GGTCCTGTTC CGCGCGGGCA GCGGCAAGCC CCTGGCCGTG 
CTCTGCCTGA CGGTGGAGCT GCAGCCTCAC GTGGTGGACC AGGTCTTCCG CTTCCACCAC 
CCGGAGCTCA CCTTCCTGAA GAAAGCCATC CGCCTGCCAC CCTGGCACAC GCTTCCAGGT 
GCCCCTGTAG GGACTTCCAG TGAGGACCCC CCTTTCCATG TTCGATGTAG TGACCCGAAC 
ATCATTTGTG AGACACAGAG TGTGGGCCCC GGGGAGCCCC GTGACGTGTT CCTGAAGGTG 
GCCAGTGGCC CTAGCCCAGA GGTCAAGGAC TTCTTTGTCA CCATCTACGT AGACCGCTGG 
TTAGCAACAC CTGTCCAGAC CTGGCAGGTC CACCTGCACT CGCTGCAGCG AGTGGACGTC 
TCCTGTATCA CTGGCCAGAG GACCCGCCTG GCTCTCGTCC TCCGGGGGAC GCAGACAGTT 
AGGAGAGTGC AGGCTTTCAG CTCACACCCC CAGGAGCTGA CGACGGACCC CAGAGGTGTG 
TTCGTGCTGC CTCCTCACGG GGTGCAGGAC TTGCATGTGA GCGTCAGGCC CTGCAGGCCG 
GGCAGCCGCT TCGCGCACCT CCACCTGGTT GACGTGGACG CCCACCAGCT GGTGGCCTCG 
TGGCTCCTGT GTCTCTCCTG CCGGCCGCCT CTCATCTCCA AGGCCTTCGA GATAAAGCTG 
CCCGCGGGGA GCGGGAGGGG CGCCAATAAG CGCATCACCT ACACCAATCC CTACCCCTCG 
CGGAGGGCCT ACCGCCTGCA CAGCGACCAC CCCCACCTGC TGCAGTTCAA GGAGGACGCC 
TTCCAGGTCG GGGGAGGTGA GACCTATACC ATCGGCCTGC GGTTTGTGTC CCGTCAGAGA 
GCCGGGGAGG AAGAGATCCT GATTTACATC AACGACCACG AAGACAAAAA CGAGGAGACC 
TTTTGCGTGA AGGTCATCTA CCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	NPHP4
Entrez Gene ID	519551
Full Name	nephrocystin 4
General protein information	Preferred Names nephrocystin 4 Names nephrocystin-4 nephronophthisis 4
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Bos taurus (cattle)	NPHP4	XP_005217234.1
Caenorhabditis elegans (roundworm)	nphp-4	NP_505982.2
Pan troglodytes (chimpanzee)	NPHP4	XP_003307840.2
	NPHP4	XP_002802192.1
Homo sapiens (human)	NPHP4	NP_055917.1
Canis lupus familiaris (dog)	NPHP4	NP_001129260.1
Mus musculus (house mouse)	Nphp4	NP_700473.2
Danio rerio (zebrafish)	nphp4	XP_005167009.1
Rattus norvegicus (Norway rat)	Nphp4	NP_001032739.2
Gallus gallus (chicken)	NPHP4	XP_003642568.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

NPHP4 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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