GLI3 cDNA ORF clone, Bos taurus(cattle)

The following GLI3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GLI3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa03954 XM_005205661.4
Latest version!
Bos taurus GLI family zinc finger 3 (GLI3), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa03954 XM_001253425.4
Latest version!
Bos taurus GLI family zinc finger 3 (GLI3), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa18737 XM_005205659.4
Latest version!
Bos taurus GLI family zinc finger 3 (GLI3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa18737 XM_015463085.1
Latest version!
Bos taurus GLI family zinc finger 3 (GLI3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
View Old Accession Versions >>
OBa03954 XM_005205661.3 Bos taurus GLI family zinc finger 3 (GLI3), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa18737 XM_005205659.3 Bos taurus GLI family zinc finger 3 (GLI3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa03954
    Clone ID Related Accession (Same CDS sequence) XM_001253425.4 , XM_005205661.3 , XM_005205661.4
    Accession Version XM_001253425.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4587bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product transcriptional activator GLI3 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644732.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_001253425.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCAGCCAC AGATTGCCCC GGGGCTCAGC AAGATCAGCG AGGAGCCTTC AACATCCAGC 
    GACGAGAGGG CCTCACTGAT CAAGAAGGAG ATCCATGGGT CCTTGCCCCA CCTGGCCGAG
    CCCTCTGTCC CCTACCGCGG GGCTGTGTTT GCTATGGACC CCAGGAATGG CTACATGGAG
    CCTCACTATC ACCCTCCTCA TCTCTTTCCT GCATTCCATC CTCCTGTACC AATTGATGCC
    AGACACCACG AGGGCCGTTA CCATTATGAT CCATCTCCGA TCCCTCCGTT GCATGTGCCT
    TCTGCCTTAT CTAGTAGCCC CACGTATTCT GACCTGCCCT TCATCAGGAT CTCCCCACAT
    CGGAACCCTG CTGCAGCTTC CGAATCCCCC TTCAGCCCCC CGCATCCCTA CATCAACCCC
    TACATGGACT ACATCCGGTC CCTGCACAGC AGCCCATCCC TCTCCATGAT CTCGGCCGCT
    CGTGGGCTGA GCCCCACAGA TGCCCCCCAT GCCGGAGTCA GCCCAGCAGA ATACTATCAT
    CAGATGGCCC TGTTAGCCGG CCAGCGCAGC CCCTATGCAG ACATCATTCC CTCTGCTGCC
    ACCGCTGGCG CAGGGGCCAT CCACATGGAG TATCTTCACG CCATGGATAG CACCAGATTC
    CCCAGCCCTA GGCTGTCAGC CCGGCCGAGC CGAAAACGTA CACTGTCCAT ATCACCACTG
    TCCGATCATA GCTTTGACCT TCAGACCATG ATAAGGACAT CTCCCAACTC CTTGGTCACG
    ATTCTCAATA ATTCCCGCAG CAGCTCTTCA GCAAGTGGCT CCTATGGCCA CTTATCTGCA
    AGTGCAATCA GCCCTGCCCT GAGCTTCACC TACCCTTCGG CGCCCGTGTC CCTCCACATG
    CATCAGCAGA TCCTAAGCCG ACAACAGAGC TTAGGCTCAG CCTTTGGACA CAGCCCTCCG
    CTCATCCACC CCGCCCCAAC TTTCCCGACC CAGAGGCCTA TTCCCGGAAT CCCTACAGTT
    CTGAACCCCG TCCAGGTCAG CTCCGGCCCT TCCGAGTCCT CACAGAACAA GCCCACGAGT
    GAGTCTGCAG TGAGCAGTAC GGGGGACCTG GTGCACAACA AACGCTCCAA GATCAAACCA
    GATGAAGACC TCCCCAGCCC AGGGCCACGG GGCCAGCAGG AACAGCCAGA AGGAACAACC
    CTGGTGAAGG AGGAAGGGGA CAAAGATGAA AGCAAACAGG AGCCCGAAGT CATCTATGAG
    ACAAACTGCC ACTGGGAAGG CTGTTCTCGA GAGTTCGACA CCCAGGAGCA GCTGGTGCAT
    CATATAAATA ATGACCACAT CCATGGAGAG AAGAAGGAGT TTGTGTGCCG GTGGCTCGAC
    TGCTCCAGAG AACAGAAACC CTTCAAAGCC CAGTATATGC TGGTGGTGCA CATGCGCAGG
    CACACGGGGG AGAAGCCTCA CAAATGCACT TTTGAAGGTT GCACAAAGGC CTACTCGAGA
    CTCGAAAACT TGAAAACACA CTTGAGATCT CACACTGGAG AGAAACCATA CGTCTGTGAG
    CACGAAGGAT GCAACAAGGC TTTCTCTAAT GCCTCGGATC GCGCTAAGCA CCAGAACAGG
    ACACATTCCA ACGAGAAACC ATATGTGTGC AAAATCCCCG GCTGCACCAA GCGCTACACA
    GACCCCAGCT CCCTCCGGAA ACACGTGAAG ACCGTGCACG GCCCAGAGGC TCATGTCACC
    AAGAAGCAGC GAGGGGACGT GCATCCCCGG CCCCCGCCCC CTCGAGATTC CGGCAGCCAC
    TCACAGTCTA GGTCACCTGG CCGGCAGACT CAGGGAGCCC TCGGGGAGCA GAAGGACCTC
    AGCAACACTA CCTCAAAGCG GGAAGAATGC CTCCAAGTGA AAACCGTCAA GGCGGAGAAG
    CCCATGACAT CTCAGCCAAG CCCTGGTGGT CAGTCTTCAT GCAGCAGCCA ACAGTCCCCC
    ATCAGCAACT ATTCCAACAG TGGGCTCGAG CTTCCTCTGA CCGATGGAGG TGGTGTGGGC
    GACCTCAGTG CCATCGATGA AACCCCAATC ATGGACTCGA CCATTTCCAC TGCAACCACA
    GCCCTCGCTT TGCAAGCCCG GAGGAACCCA GCAGGGACCA AATGGATGGA GCACGTCAAA
    CTAGAAAGGC TCAAACAAGT GAATGGAATG CTTCCACGAC TGAACCCCAT TCTACCCCCT
    AAAGCCCCTG CAGTCTCTCC TCTCATAGGA AACGGCACGC AGCCCAGTAC CAGCTGCAGT
    CTGGGCGGGC CCATGCCCCT CCTCCCGAAC AGAAGCGACC TGTCTGGGGT GGACATCACC
    ATGTTGAACA TGCTCAACCG GAGGGACAGC AGCACCAGCA CCGTCAGCTC CGCCTACCTG
    AGCAGCCGCC GCTCCTCTGG CATCTCGCCC TGCTTCTCCA GCCGCCGGTC CAGCGAGGCG
    TCCCAGGCCG AGGGGCGACC CCAGAACGTG AGCGTGGCCG ACTCTTACGA CCCCATCTCC
    ACCGACGCCT CTCGCAGGTC CAGCGAGGCC AGCCAGGGCG ACGGTCTGCC CAGCCTGCTC
    AGCCTCACTC CCGCCCAGCA GTACCGCCTG AAAGCCAAGT ACGCTGCCGC CACGGGCGGC
    CCCCCGCCCA CCCCGCTGCC CAACATGGAG AGGATGAGCC TCAAGACCCG GATGGCCCTG
    TTGGGAGATG GCAGGGAGCC GGCGGGGACC CTGCCTCCCG TGCATCCTCC TCGGAGATGC
    AGCGACGGCG GGGCCCACGG TTACAGCCGG CGCCCACTGC TGCCCCAGGA TGCGCTGGGC
    AACGGCGTGA GAAGAGCCAG CGACCCGGTG AGGACGGTTT CGGAGAGCCT CTCTCTGCCC
    CGGGTACAGC GGTTCAACAG CCTTAACAGC TTCAACCCTC CGGTGTTGCC TCCATCCCTG
    GAAAAGCGCA ACCTCGTGCT TCAGAACTAC ACGCGGCCCG ATGGGGGCCA GGCCCGCCAC
    TTCCACTCCT CTCCCTGTCC CCCGAGCATC ACTGAGAATG TCACCCTGGA GTCCCTGACC
    ATGGACGCCG ACGTGAGCCT GAACGATGAG GACCTCCTGC CTGATGACGT GGTGCAGTAT
    TTAAATTCCC AGAACCCAGC CGGGTATGAG CAGCACTTCC CAGGCACCTT CTCTGATGAC
    AGCAAAGTGC CCCATGGCTT AGGGAGTGGA CCCGGGGACT TTGACCCACT GGGGCTGCCC
    GACAGCCACG CCAGCCACAC CGGCAGCCAG CAGTACCGCA GCCTGGAGCA GCCCTGCCCC
    GATGCCAGTA AATCCGACCT GCCCATTCAG TGGAACGAAG TCAGCTCCGG CAGCGCGGAC
    CTGTCCTCCT CCAAGCTGAA ATGTGGCCAG CGGTCCATGG GGCAGCAGGC CCGGGCCTTC
    GGGTCGTACA GCCACATGGG TGTTCACCCA CAGAATCCAT TGAGGAGTGG GGCTGGCCCA
    GCTGGGGGGT ATCCGACCCT TGGGGAGCAT GCCAGCTCCT TCGGGGGCCC GGAACACTTA
    GTGAGCCACG GTGGCGGAGC TGGCACCAAT GGGAATGCCT TCCATGAACC ACCCTATAAG
    GCCCAGCAGT ATGGGAACTG CCTCAACAGG CAGCCTGGTG CCCCCACCTT ACTCGATGGG
    GCCTGTGGCC CTGGGATCCA GGCGGCAAAG CTGAAAAGCA TCACCATGCA AGGGAGCGGG
    AGCCATCTGG ATTTTGGCCT GTCGGTGGTG CCGAATGAGT CAACCGGCAG CACGGTGAAT
    GGCATACCCA CCCCAGACCC CACAGGACAG GGGTACCTGG CTCAGCAGCT CCTCGGTGAC
    AGCACACACA CCCAGGGGGC AGGCCGCTCC GGGCAGAACC TGCTGGGGCA GGCTAGTGCT
    ACCTCACACA TCTATCAAGG GCCGGAGAGC AGCCTGCCCG GGCCTCCCAG CATCAGCGTG
    CAGCCGCCAA ACGTGGCAGC CGTCAGGGGC TACCAGCCAT GTGTTGGCTA CGGGGGCAGC
    CGGCGCCAGG CTGGGCCGAG GGGTGGTCTC GCTCTGCAGC CAGGACAGTT TAGTGACCCG
    AGTCAGACCT GCAGGGTGAA TGGCATCAAG ATGGAGATGC CAGGACAGCC CCATCAGCTC
    TGTTCGAATG TGCAGAGTTA CTCTGGTCAG TTCTATGACC AAACTGTTGG CTTCGGTCAG
    CAAGACATGA AAACTGGTTC CTTCTCCATG TCAGAAGCCA ACTGCCTGCT CCAGGGGGCC
    GGCGCCGAAA ACTCTGAATT ACTCTCCCCA GGTGCTAACC AGGTGACAAG CACAGTTGAC
    AGCCTCGACA GCCATGACCT GGAAGGGGTG CAGATTGATT TCGACGCTAT CATAGACGAC
    GGGGACCACG CCAGCCTGAT GTCAGGAGCC CTGAGCCCAA GCATTATTCA GAACCTTTCT
    CATAGCTCCT CCCGACTCAC CACGCCGCGA GCCGCCCTTC CGTTCCCGCC TCTGTCTTTG
    AGCACCACCA ACATGGCAAT CGGGGACATG AGCTCTCTGC TGACCTCCCT CGCGGAAGAA
    AGCAAATTCC TTGCGGTTAT GCAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_001253426.2
    CDS311..4897
    Translation

    Target ORF information:

    RefSeq Version XM_001253425.4
    Organism Bos taurus(cattle)
    Definition Bos taurus GLI family zinc finger 3 (GLI3), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_001253425.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCAGCCAC AGATTGCCCC GGGGCTCAGC AAGATCAGCG AGGAGCCTTC AACATCCAGC 
    GACGAGAGGG CCTCACTGAT CAAGAAGGAG ATCCATGGGT CCTTGCCCCA CCTGGCCGAG
    CCCTCTGTCC CCTACCGCGG GGCTGTGTTT GCTATGGACC CCAGGAATGG CTACATGGAG
    CCTCACTATC ACCCTCCTCA TCTCTTTCCT GCATTCCATC CTCCTGTACC AATTGATGCC
    AGACACCACG AGGGCCGTTA CCATTATGAT CCATCTCCGA TCCCTCCGTT GCATGTGCCT
    TCTGCCTTAT CTAGTAGCCC CACGTATTCT GACCTGCCCT TCATCAGGAT CTCCCCACAT
    CGGAACCCTG CTGCAGCTTC CGAATCCCCC TTCAGCCCCC CGCATCCCTA CATCAACCCC
    TACATGGACT ACATCCGGTC CCTGCACAGC AGCCCATCCC TCTCCATGAT CTCGGCCGCT
    CGTGGGCTGA GCCCCACAGA TGCCCCCCAT GCCGGAGTCA GCCCAGCAGA ATACTATCAT
    CAGATGGCCC TGTTAGCCGG CCAGCGCAGC CCCTATGCAG ACATCATTCC CTCTGCTGCC
    ACCGCTGGCG CAGGGGCCAT CCACATGGAG TATCTTCACG CCATGGATAG CACCAGATTC
    CCCAGCCCTA GGCTGTCAGC CCGGCCGAGC CGAAAACGTA CACTGTCCAT ATCACCACTG
    TCCGATCATA GCTTTGACCT TCAGACCATG ATAAGGACAT CTCCCAACTC CTTGGTCACG
    ATTCTCAATA ATTCCCGCAG CAGCTCTTCA GCAAGTGGCT CCTATGGCCA CTTATCTGCA
    AGTGCAATCA GCCCTGCCCT GAGCTTCACC TACCCTTCGG CGCCCGTGTC CCTCCACATG
    CATCAGCAGA TCCTAAGCCG ACAACAGAGC TTAGGCTCAG CCTTTGGACA CAGCCCTCCG
    CTCATCCACC CCGCCCCAAC TTTCCCGACC CAGAGGCCTA TTCCCGGAAT CCCTACAGTT
    CTGAACCCCG TCCAGGTCAG CTCCGGCCCT TCCGAGTCCT CACAGAACAA GCCCACGAGT
    GAGTCTGCAG TGAGCAGTAC GGGGGACCTG GTGCACAACA AACGCTCCAA GATCAAACCA
    GATGAAGACC TCCCCAGCCC AGGGCCACGG GGCCAGCAGG AACAGCCAGA AGGAACAACC
    CTGGTGAAGG AGGAAGGGGA CAAAGATGAA AGCAAACAGG AGCCCGAAGT CATCTATGAG
    ACAAACTGCC ACTGGGAAGG CTGTTCTCGA GAGTTCGACA CCCAGGAGCA GCTGGTGCAT
    CATATAAATA ATGACCACAT CCATGGAGAG AAGAAGGAGT TTGTGTGCCG GTGGCTCGAC
    TGCTCCAGAG AACAGAAACC CTTCAAAGCC CAGTATATGC TGGTGGTGCA CATGCGCAGG
    CACACGGGGG AGAAGCCTCA CAAATGCACT TTTGAAGGTT GCACAAAGGC CTACTCGAGA
    CTCGAAAACT TGAAAACACA CTTGAGATCT CACACTGGAG AGAAACCATA CGTCTGTGAG
    CACGAAGGAT GCAACAAGGC TTTCTCTAAT GCCTCGGATC GCGCTAAGCA CCAGAACAGG
    ACACATTCCA ACGAGAAACC ATATGTGTGC AAAATCCCCG GCTGCACCAA GCGCTACACA
    GACCCCAGCT CCCTCCGGAA ACACGTGAAG ACCGTGCACG GCCCAGAGGC TCATGTCACC
    AAGAAGCAGC GAGGGGACGT GCATCCCCGG CCCCCGCCCC CTCGAGATTC CGGCAGCCAC
    TCACAGTCTA GGTCACCTGG CCGGCAGACT CAGGGAGCCC TCGGGGAGCA GAAGGACCTC
    AGCAACACTA CCTCAAAGCG GGAAGAATGC CTCCAAGTGA AAACCGTCAA GGCGGAGAAG
    CCCATGACAT CTCAGCCAAG CCCTGGTGGT CAGTCTTCAT GCAGCAGCCA ACAGTCCCCC
    ATCAGCAACT ATTCCAACAG TGGGCTCGAG CTTCCTCTGA CCGATGGAGG TGGTGTGGGC
    GACCTCAGTG CCATCGATGA AACCCCAATC ATGGACTCGA CCATTTCCAC TGCAACCACA
    GCCCTCGCTT TGCAAGCCCG GAGGAACCCA GCAGGGACCA AATGGATGGA GCACGTCAAA
    CTAGAAAGGC TCAAACAAGT GAATGGAATG CTTCCACGAC TGAACCCCAT TCTACCCCCT
    AAAGCCCCTG CAGTCTCTCC TCTCATAGGA AACGGCACGC AGCCCAGTAC CAGCTGCAGT
    CTGGGCGGGC CCATGCCCCT CCTCCCGAAC AGAAGCGACC TGTCTGGGGT GGACATCACC
    ATGTTGAACA TGCTCAACCG GAGGGACAGC AGCACCAGCA CCGTCAGCTC CGCCTACCTG
    AGCAGCCGCC GCTCCTCTGG CATCTCGCCC TGCTTCTCCA GCCGCCGGTC CAGCGAGGCG
    TCCCAGGCCG AGGGGCGACC CCAGAACGTG AGCGTGGCCG ACTCTTACGA CCCCATCTCC
    ACCGACGCCT CTCGCAGGTC CAGCGAGGCC AGCCAGGGCG ACGGTCTGCC CAGCCTGCTC
    AGCCTCACTC CCGCCCAGCA GTACCGCCTG AAAGCCAAGT ACGCTGCCGC CACGGGCGGC
    CCCCCGCCCA CCCCGCTGCC CAACATGGAG AGGATGAGCC TCAAGACCCG GATGGCCCTG
    TTGGGAGATG GCAGGGAGCC GGCGGGGACC CTGCCTCCCG TGCATCCTCC TCGGAGATGC
    AGCGACGGCG GGGCCCACGG TTACAGCCGG CGCCCACTGC TGCCCCAGGA TGCGCTGGGC
    AACGGCGTGA GAAGAGCCAG CGACCCGGTG AGGACGGTTT CGGAGAGCCT CTCTCTGCCC
    CGGGTACAGC GGTTCAACAG CCTTAACAGC TTCAACCCTC CGGTGTTGCC TCCATCCCTG
    GAAAAGCGCA ACCTCGTGCT TCAGAACTAC ACGCGGCCCG ATGGGGGCCA GGCCCGCCAC
    TTCCACTCCT CTCCCTGTCC CCCGAGCATC ACTGAGAATG TCACCCTGGA GTCCCTGACC
    ATGGACGCCG ACGTGAGCCT GAACGATGAG GACCTCCTGC CTGATGACGT GGTGCAGTAT
    TTAAATTCCC AGAACCCAGC CGGGTATGAG CAGCACTTCC CAGGCACCTT CTCTGATGAC
    AGCAAAGTGC CCCATGGCTT AGGGAGTGGA CCCGGGGACT TTGACCCACT GGGGCTGCCC
    GACAGCCACG CCAGCCACAC CGGCAGCCAG CAGTACCGCA GCCTGGAGCA GCCCTGCCCC
    GATGCCAGTA AATCCGACCT GCCCATTCAG TGGAACGAAG TCAGCTCCGG CAGCGCGGAC
    CTGTCCTCCT CCAAGCTGAA ATGTGGCCAG CGGTCCATGG GGCAGCAGGC CCGGGCCTTC
    GGGTCGTACA GCCACATGGG TGTTCACCCA CAGAATCCAT TGAGGAGTGG GGCTGGCCCA
    GCTGGGGGGT ATCCGACCCT TGGGGAGCAT GCCAGCTCCT TCGGGGGCCC GGAACACTTA
    GTGAGCCACG GTGGCGGAGC TGGCACCAAT GGGAATGCCT TCCATGAACC ACCCTATAAG
    GCCCAGCAGT ATGGGAACTG CCTCAACAGG CAGCCTGGTG CCCCCACCTT ACTCGATGGG
    GCCTGTGGCC CTGGGATCCA GGCGGCAAAG CTGAAAAGCA TCACCATGCA AGGGAGCGGG
    AGCCATCTGG ATTTTGGCCT GTCGGTGGTG CCGAATGAGT CAACCGGCAG CACGGTGAAT
    GGCATACCCA CCCCAGACCC CACAGGACAG GGGTACCTGG CTCAGCAGCT CCTCGGTGAC
    AGCACACACA CCCAGGGGGC AGGCCGCTCC GGGCAGAACC TGCTGGGGCA GGCTAGTGCT
    ACCTCACACA TCTATCAAGG GCCGGAGAGC AGCCTGCCCG GGCCTCCCAG CATCAGCGTG
    CAGCCGCCAA ACGTGGCAGC CGTCAGGGGC TACCAGCCAT GTGTTGGCTA CGGGGGCAGC
    CGGCGCCAGG CTGGGCCGAG GGGTGGTCTC GCTCTGCAGC CAGGACAGTT TAGTGACCCG
    AGTCAGACCT GCAGGGTGAA TGGCATCAAG ATGGAGATGC CAGGACAGCC CCATCAGCTC
    TGTTCGAATG TGCAGAGTTA CTCTGGTCAG TTCTATGACC AAACTGTTGG CTTCGGTCAG
    CAAGACATGA AAACTGGTTC CTTCTCCATG TCAGAAGCCA ACTGCCTGCT CCAGGGGGCC
    GGCGCCGAAA ACTCTGAATT ACTCTCCCCA GGTGCTAACC AGGTGACAAG CACAGTTGAC
    AGCCTCGACA GCCATGACCT GGAAGGGGTG CAGATTGATT TCGACGCTAT CATAGACGAC
    GGGGACCACG CCAGCCTGAT GTCAGGAGCC CTGAGCCCAA GCATTATTCA GAACCTTTCT
    CATAGCTCCT CCCGACTCAC CACGCCGCGA GCCGCCCTTC CGTTCCCGCC TCTGTCTTTG
    AGCACCACCA ACATGGCAAT CGGGGACATG AGCTCTCTGC TGACCTCCCT CGCGGAAGAA
    AGCAAATTCC TTGCGGTTAT GCAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa03954
    Clone ID Related Accession (Same CDS sequence) XM_001253425.4 , XM_005205661.3 , XM_005205661.4
    Accession Version XM_005205661.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4587bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product transcriptional activator GLI3 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103903.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005205661.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCAGCCAC AGATTGCCCC GGGGCTCAGC AAGATCAGCG AGGAGCCTTC AACATCCAGC 
    GACGAGAGGG CCTCACTGAT CAAGAAGGAG ATCCATGGGT CCTTGCCCCA CCTGGCCGAG
    CCCTCTGTCC CCTACCGCGG GGCTGTGTTT GCTATGGACC CCAGGAATGG CTACATGGAG
    CCTCACTATC ACCCTCCTCA TCTCTTTCCT GCATTCCATC CTCCTGTACC AATTGATGCC
    AGACACCACG AGGGCCGTTA CCATTATGAT CCATCTCCGA TCCCTCCGTT GCATGTGCCT
    TCTGCCTTAT CTAGTAGCCC CACGTATTCT GACCTGCCCT TCATCAGGAT CTCCCCACAT
    CGGAACCCTG CTGCAGCTTC CGAATCCCCC TTCAGCCCCC CGCATCCCTA CATCAACCCC
    TACATGGACT ACATCCGGTC CCTGCACAGC AGCCCATCCC TCTCCATGAT CTCGGCCGCT
    CGTGGGCTGA GCCCCACAGA TGCCCCCCAT GCCGGAGTCA GCCCAGCAGA ATACTATCAT
    CAGATGGCCC TGTTAGCCGG CCAGCGCAGC CCCTATGCAG ACATCATTCC CTCTGCTGCC
    ACCGCTGGCG CAGGGGCCAT CCACATGGAG TATCTTCACG CCATGGATAG CACCAGATTC
    CCCAGCCCTA GGCTGTCAGC CCGGCCGAGC CGAAAACGTA CACTGTCCAT ATCACCACTG
    TCCGATCATA GCTTTGACCT TCAGACCATG ATAAGGACAT CTCCCAACTC CTTGGTCACG
    ATTCTCAATA ATTCCCGCAG CAGCTCTTCA GCAAGTGGCT CCTATGGCCA CTTATCTGCA
    AGTGCAATCA GCCCTGCCCT GAGCTTCACC TACCCTTCGG CGCCCGTGTC CCTCCACATG
    CATCAGCAGA TCCTAAGCCG ACAACAGAGC TTAGGCTCAG CCTTTGGACA CAGCCCTCCG
    CTCATCCACC CCGCCCCAAC TTTCCCGACC CAGAGGCCTA TTCCCGGAAT CCCTACAGTT
    CTGAACCCCG TCCAGGTCAG CTCCGGCCCT TCCGAGTCCT CACAGAACAA GCCCACGAGT
    GAGTCTGCAG TGAGCAGTAC GGGGGACCTG GTGCACAACA AACGCTCCAA GATCAAACCA
    GATGAAGACC TCCCCAGCCC AGGGCCACGG GGCCAGCAGG AACAGCCAGA AGGAACAACC
    CTGGTGAAGG AGGAAGGGGA CAAAGATGAA AGCAAACAGG AGCCCGAAGT CATCTATGAG
    ACAAACTGCC ACTGGGAAGG CTGTTCTCGA GAGTTCGACA CCCAGGAGCA GCTGGTGCAT
    CATATAAATA ATGACCACAT CCATGGAGAG AAGAAGGAGT TTGTGTGCCG GTGGCTCGAC
    TGCTCCAGAG AACAGAAACC CTTCAAAGCC CAGTATATGC TGGTGGTGCA CATGCGCAGG
    CACACGGGGG AGAAGCCTCA CAAATGCACT TTTGAAGGTT GCACAAAGGC CTACTCGAGA
    CTCGAAAACT TGAAAACACA CTTGAGATCT CACACTGGAG AGAAACCATA CGTCTGTGAG
    CACGAAGGAT GCAACAAGGC TTTCTCTAAT GCCTCGGATC GCGCTAAGCA CCAGAACAGG
    ACACATTCCA ACGAGAAACC ATATGTGTGC AAAATCCCCG GCTGCACCAA GCGCTACACA
    GACCCCAGCT CCCTCCGGAA ACACGTGAAG ACCGTGCACG GCCCAGAGGC TCATGTCACC
    AAGAAGCAGC GAGGGGACGT GCATCCCCGG CCCCCGCCCC CTCGAGATTC CGGCAGCCAC
    TCACAGTCTA GGTCACCTGG CCGGCAGACT CAGGGAGCCC TCGGGGAGCA GAAGGACCTC
    AGCAACACTA CCTCAAAGCG GGAAGAATGC CTCCAAGTGA AAACCGTCAA GGCGGAGAAG
    CCCATGACAT CTCAGCCAAG CCCTGGTGGT CAGTCTTCAT GCAGCAGCCA ACAGTCCCCC
    ATCAGCAACT ATTCCAACAG TGGGCTCGAG CTTCCTCTGA CCGATGGAGG TGGTGTGGGC
    GACCTCAGTG CCATCGATGA AACCCCAATC ATGGACTCGA CCATTTCCAC TGCAACCACA
    GCCCTCGCTT TGCAAGCCCG GAGGAACCCA GCAGGGACCA AATGGATGGA GCACGTCAAA
    CTAGAAAGGC TCAAACAAGT GAATGGAATG CTTCCACGAC TGAACCCCAT TCTACCCCCT
    AAAGCCCCTG CAGTCTCTCC TCTCATAGGA AACGGCACGC AGCCCAGTAC CAGCTGCAGT
    CTGGGCGGGC CCATGCCCCT CCTCCCGAAC AGAAGCGACC TGTCTGGGGT GGACATCACC
    ATGTTGAACA TGCTCAACCG GAGGGACAGC AGCACCAGCA CCGTCAGCTC CGCCTACCTG
    AGCAGCCGCC GCTCCTCTGG CATCTCGCCC TGCTTCTCCA GCCGCCGGTC CAGCGAGGCG
    TCCCAGGCCG AGGGGCGACC CCAGAACGTG AGCGTGGCCG ACTCTTACGA CCCCATCTCC
    ACCGACGCCT CTCGCAGGTC CAGCGAGGCC AGCCAGGGCG ACGGTCTGCC CAGCCTGCTC
    AGCCTCACTC CCGCCCAGCA GTACCGCCTG AAAGCCAAGT ACGCTGCCGC CACGGGCGGC
    CCCCCGCCCA CCCCGCTGCC CAACATGGAG AGGATGAGCC TCAAGACCCG GATGGCCCTG
    TTGGGAGATG GCAGGGAGCC GGCGGGGACC CTGCCTCCCG TGCATCCTCC TCGGAGATGC
    AGCGACGGCG GGGCCCACGG TTACAGCCGG CGCCCACTGC TGCCCCAGGA TGCGCTGGGC
    AACGGCGTGA GAAGAGCCAG CGACCCGGTG AGGACGGTTT CGGAGAGCCT CTCTCTGCCC
    CGGGTACAGC GGTTCAACAG CCTTAACAGC TTCAACCCTC CGGTGTTGCC TCCATCCCTG
    GAAAAGCGCA ACCTCGTGCT TCAGAACTAC ACGCGGCCCG ATGGGGGCCA GGCCCGCCAC
    TTCCACTCCT CTCCCTGTCC CCCGAGCATC ACTGAGAATG TCACCCTGGA GTCCCTGACC
    ATGGACGCCG ACGTGAGCCT GAACGATGAG GACCTCCTGC CTGATGACGT GGTGCAGTAT
    TTAAATTCCC AGAACCCAGC CGGGTATGAG CAGCACTTCC CAGGCACCTT CTCTGATGAC
    AGCAAAGTGC CCCATGGCTT AGGGAGTGGA CCCGGGGACT TTGACCCACT GGGGCTGCCC
    GACAGCCACG CCAGCCACAC CGGCAGCCAG CAGTACCGCA GCCTGGAGCA GCCCTGCCCC
    GATGCCAGTA AATCCGACCT GCCCATTCAG TGGAACGAAG TCAGCTCCGG CAGCGCGGAC
    CTGTCCTCCT CCAAGCTGAA ATGTGGCCAG CGGTCCATGG GGCAGCAGGC CCGGGCCTTC
    GGGTCGTACA GCCACATGGG TGTTCACCCA CAGAATCCAT TGAGGAGTGG GGCTGGCCCA
    GCTGGGGGGT ATCCGACCCT TGGGGAGCAT GCCAGCTCCT TCGGGGGCCC GGAACACTTA
    GTGAGCCACG GTGGCGGAGC TGGCACCAAT GGGAATGCCT TCCATGAACC ACCCTATAAG
    GCCCAGCAGT ATGGGAACTG CCTCAACAGG CAGCCTGGTG CCCCCACCTT ACTCGATGGG
    GCCTGTGGCC CTGGGATCCA GGCGGCAAAG CTGAAAAGCA TCACCATGCA AGGGAGCGGG
    AGCCATCTGG ATTTTGGCCT GTCGGTGGTG CCGAATGAGT CAACCGGCAG CACGGTGAAT
    GGCATACCCA CCCCAGACCC CACAGGACAG GGGTACCTGG CTCAGCAGCT CCTCGGTGAC
    AGCACACACA CCCAGGGGGC AGGCCGCTCC GGGCAGAACC TGCTGGGGCA GGCTAGTGCT
    ACCTCACACA TCTATCAAGG GCCGGAGAGC AGCCTGCCCG GGCCTCCCAG CATCAGCGTG
    CAGCCGCCAA ACGTGGCAGC CGTCAGGGGC TACCAGCCAT GTGTTGGCTA CGGGGGCAGC
    CGGCGCCAGG CTGGGCCGAG GGGTGGTCTC GCTCTGCAGC CAGGACAGTT TAGTGACCCG
    AGTCAGACCT GCAGGGTGAA TGGCATCAAG ATGGAGATGC CAGGACAGCC CCATCAGCTC
    TGTTCGAATG TGCAGAGTTA CTCTGGTCAG TTCTATGACC AAACTGTTGG CTTCGGTCAG
    CAAGACATGA AAACTGGTTC CTTCTCCATG TCAGAAGCCA ACTGCCTGCT CCAGGGGGCC
    GGCGCCGAAA ACTCTGAATT ACTCTCCCCA GGTGCTAACC AGGTGACAAG CACAGTTGAC
    AGCCTCGACA GCCATGACCT GGAAGGGGTG CAGATTGATT TCGACGCTAT CATAGACGAC
    GGGGACCACG CCAGCCTGAT GTCAGGAGCC CTGAGCCCAA GCATTATTCA GAACCTTTCT
    CATAGCTCCT CCCGACTCAC CACGCCGCGA GCCGCCCTTC CGTTCCCGCC TCTGTCTTTG
    AGCACCACCA ACATGGCAAT CGGGGACATG AGCTCTCTGC TGACCTCCCT CGCGGAAGAA
    AGCAAATTCC TTGCGGTTAT GCAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005205718.1
    CDS311..4897
    Translation

    Target ORF information:

    RefSeq Version XM_005205661.3
    Organism Bos taurus(cattle)
    Definition Bos taurus GLI family zinc finger 3 (GLI3), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005205661.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCAGCCAC AGATTGCCCC GGGGCTCAGC AAGATCAGCG AGGAGCCTTC AACATCCAGC 
    GACGAGAGGG CCTCACTGAT CAAGAAGGAG ATCCATGGGT CCTTGCCCCA CCTGGCCGAG
    CCCTCTGTCC CCTACCGCGG GGCTGTGTTT GCTATGGACC CCAGGAATGG CTACATGGAG
    CCTCACTATC ACCCTCCTCA TCTCTTTCCT GCATTCCATC CTCCTGTACC AATTGATGCC
    AGACACCACG AGGGCCGTTA CCATTATGAT CCATCTCCGA TCCCTCCGTT GCATGTGCCT
    TCTGCCTTAT CTAGTAGCCC CACGTATTCT GACCTGCCCT TCATCAGGAT CTCCCCACAT
    CGGAACCCTG CTGCAGCTTC CGAATCCCCC TTCAGCCCCC CGCATCCCTA CATCAACCCC
    TACATGGACT ACATCCGGTC CCTGCACAGC AGCCCATCCC TCTCCATGAT CTCGGCCGCT
    CGTGGGCTGA GCCCCACAGA TGCCCCCCAT GCCGGAGTCA GCCCAGCAGA ATACTATCAT
    CAGATGGCCC TGTTAGCCGG CCAGCGCAGC CCCTATGCAG ACATCATTCC CTCTGCTGCC
    ACCGCTGGCG CAGGGGCCAT CCACATGGAG TATCTTCACG CCATGGATAG CACCAGATTC
    CCCAGCCCTA GGCTGTCAGC CCGGCCGAGC CGAAAACGTA CACTGTCCAT ATCACCACTG
    TCCGATCATA GCTTTGACCT TCAGACCATG ATAAGGACAT CTCCCAACTC CTTGGTCACG
    ATTCTCAATA ATTCCCGCAG CAGCTCTTCA GCAAGTGGCT CCTATGGCCA CTTATCTGCA
    AGTGCAATCA GCCCTGCCCT GAGCTTCACC TACCCTTCGG CGCCCGTGTC CCTCCACATG
    CATCAGCAGA TCCTAAGCCG ACAACAGAGC TTAGGCTCAG CCTTTGGACA CAGCCCTCCG
    CTCATCCACC CCGCCCCAAC TTTCCCGACC CAGAGGCCTA TTCCCGGAAT CCCTACAGTT
    CTGAACCCCG TCCAGGTCAG CTCCGGCCCT TCCGAGTCCT CACAGAACAA GCCCACGAGT
    GAGTCTGCAG TGAGCAGTAC GGGGGACCTG GTGCACAACA AACGCTCCAA GATCAAACCA
    GATGAAGACC TCCCCAGCCC AGGGCCACGG GGCCAGCAGG AACAGCCAGA AGGAACAACC
    CTGGTGAAGG AGGAAGGGGA CAAAGATGAA AGCAAACAGG AGCCCGAAGT CATCTATGAG
    ACAAACTGCC ACTGGGAAGG CTGTTCTCGA GAGTTCGACA CCCAGGAGCA GCTGGTGCAT
    CATATAAATA ATGACCACAT CCATGGAGAG AAGAAGGAGT TTGTGTGCCG GTGGCTCGAC
    TGCTCCAGAG AACAGAAACC CTTCAAAGCC CAGTATATGC TGGTGGTGCA CATGCGCAGG
    CACACGGGGG AGAAGCCTCA CAAATGCACT TTTGAAGGTT GCACAAAGGC CTACTCGAGA
    CTCGAAAACT TGAAAACACA CTTGAGATCT CACACTGGAG AGAAACCATA CGTCTGTGAG
    CACGAAGGAT GCAACAAGGC TTTCTCTAAT GCCTCGGATC GCGCTAAGCA CCAGAACAGG
    ACACATTCCA ACGAGAAACC ATATGTGTGC AAAATCCCCG GCTGCACCAA GCGCTACACA
    GACCCCAGCT CCCTCCGGAA ACACGTGAAG ACCGTGCACG GCCCAGAGGC TCATGTCACC
    AAGAAGCAGC GAGGGGACGT GCATCCCCGG CCCCCGCCCC CTCGAGATTC CGGCAGCCAC
    TCACAGTCTA GGTCACCTGG CCGGCAGACT CAGGGAGCCC TCGGGGAGCA GAAGGACCTC
    AGCAACACTA CCTCAAAGCG GGAAGAATGC CTCCAAGTGA AAACCGTCAA GGCGGAGAAG
    CCCATGACAT CTCAGCCAAG CCCTGGTGGT CAGTCTTCAT GCAGCAGCCA ACAGTCCCCC
    ATCAGCAACT ATTCCAACAG TGGGCTCGAG CTTCCTCTGA CCGATGGAGG TGGTGTGGGC
    GACCTCAGTG CCATCGATGA AACCCCAATC ATGGACTCGA CCATTTCCAC TGCAACCACA
    GCCCTCGCTT TGCAAGCCCG GAGGAACCCA GCAGGGACCA AATGGATGGA GCACGTCAAA
    CTAGAAAGGC TCAAACAAGT GAATGGAATG CTTCCACGAC TGAACCCCAT TCTACCCCCT
    AAAGCCCCTG CAGTCTCTCC TCTCATAGGA AACGGCACGC AGCCCAGTAC CAGCTGCAGT
    CTGGGCGGGC CCATGCCCCT CCTCCCGAAC AGAAGCGACC TGTCTGGGGT GGACATCACC
    ATGTTGAACA TGCTCAACCG GAGGGACAGC AGCACCAGCA CCGTCAGCTC CGCCTACCTG
    AGCAGCCGCC GCTCCTCTGG CATCTCGCCC TGCTTCTCCA GCCGCCGGTC CAGCGAGGCG
    TCCCAGGCCG AGGGGCGACC CCAGAACGTG AGCGTGGCCG ACTCTTACGA CCCCATCTCC
    ACCGACGCCT CTCGCAGGTC CAGCGAGGCC AGCCAGGGCG ACGGTCTGCC CAGCCTGCTC
    AGCCTCACTC CCGCCCAGCA GTACCGCCTG AAAGCCAAGT ACGCTGCCGC CACGGGCGGC
    CCCCCGCCCA CCCCGCTGCC CAACATGGAG AGGATGAGCC TCAAGACCCG GATGGCCCTG
    TTGGGAGATG GCAGGGAGCC GGCGGGGACC CTGCCTCCCG TGCATCCTCC TCGGAGATGC
    AGCGACGGCG GGGCCCACGG TTACAGCCGG CGCCCACTGC TGCCCCAGGA TGCGCTGGGC
    AACGGCGTGA GAAGAGCCAG CGACCCGGTG AGGACGGTTT CGGAGAGCCT CTCTCTGCCC
    CGGGTACAGC GGTTCAACAG CCTTAACAGC TTCAACCCTC CGGTGTTGCC TCCATCCCTG
    GAAAAGCGCA ACCTCGTGCT TCAGAACTAC ACGCGGCCCG ATGGGGGCCA GGCCCGCCAC
    TTCCACTCCT CTCCCTGTCC CCCGAGCATC ACTGAGAATG TCACCCTGGA GTCCCTGACC
    ATGGACGCCG ACGTGAGCCT GAACGATGAG GACCTCCTGC CTGATGACGT GGTGCAGTAT
    TTAAATTCCC AGAACCCAGC CGGGTATGAG CAGCACTTCC CAGGCACCTT CTCTGATGAC
    AGCAAAGTGC CCCATGGCTT AGGGAGTGGA CCCGGGGACT TTGACCCACT GGGGCTGCCC
    GACAGCCACG CCAGCCACAC CGGCAGCCAG CAGTACCGCA GCCTGGAGCA GCCCTGCCCC
    GATGCCAGTA AATCCGACCT GCCCATTCAG TGGAACGAAG TCAGCTCCGG CAGCGCGGAC
    CTGTCCTCCT CCAAGCTGAA ATGTGGCCAG CGGTCCATGG GGCAGCAGGC CCGGGCCTTC
    GGGTCGTACA GCCACATGGG TGTTCACCCA CAGAATCCAT TGAGGAGTGG GGCTGGCCCA
    GCTGGGGGGT ATCCGACCCT TGGGGAGCAT GCCAGCTCCT TCGGGGGCCC GGAACACTTA
    GTGAGCCACG GTGGCGGAGC TGGCACCAAT GGGAATGCCT TCCATGAACC ACCCTATAAG
    GCCCAGCAGT ATGGGAACTG CCTCAACAGG CAGCCTGGTG CCCCCACCTT ACTCGATGGG
    GCCTGTGGCC CTGGGATCCA GGCGGCAAAG CTGAAAAGCA TCACCATGCA AGGGAGCGGG
    AGCCATCTGG ATTTTGGCCT GTCGGTGGTG CCGAATGAGT CAACCGGCAG CACGGTGAAT
    GGCATACCCA CCCCAGACCC CACAGGACAG GGGTACCTGG CTCAGCAGCT CCTCGGTGAC
    AGCACACACA CCCAGGGGGC AGGCCGCTCC GGGCAGAACC TGCTGGGGCA GGCTAGTGCT
    ACCTCACACA TCTATCAAGG GCCGGAGAGC AGCCTGCCCG GGCCTCCCAG CATCAGCGTG
    CAGCCGCCAA ACGTGGCAGC CGTCAGGGGC TACCAGCCAT GTGTTGGCTA CGGGGGCAGC
    CGGCGCCAGG CTGGGCCGAG GGGTGGTCTC GCTCTGCAGC CAGGACAGTT TAGTGACCCG
    AGTCAGACCT GCAGGGTGAA TGGCATCAAG ATGGAGATGC CAGGACAGCC CCATCAGCTC
    TGTTCGAATG TGCAGAGTTA CTCTGGTCAG TTCTATGACC AAACTGTTGG CTTCGGTCAG
    CAAGACATGA AAACTGGTTC CTTCTCCATG TCAGAAGCCA ACTGCCTGCT CCAGGGGGCC
    GGCGCCGAAA ACTCTGAATT ACTCTCCCCA GGTGCTAACC AGGTGACAAG CACAGTTGAC
    AGCCTCGACA GCCATGACCT GGAAGGGGTG CAGATTGATT TCGACGCTAT CATAGACGAC
    GGGGACCACG CCAGCCTGAT GTCAGGAGCC CTGAGCCCAA GCATTATTCA GAACCTTTCT
    CATAGCTCCT CCCGACTCAC CACGCCGCGA GCCGCCCTTC CGTTCCCGCC TCTGTCTTTG
    AGCACCACCA ACATGGCAAT CGGGGACATG AGCTCTCTGC TGACCTCCCT CGCGGAAGAA
    AGCAAATTCC TTGCGGTTAT GCAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa03954
    Clone ID Related Accession (Same CDS sequence) XM_001253425.4 , XM_005205661.3 , XM_005205661.4
    Accession Version XM_005205661.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4587bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product transcriptional activator GLI3 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005205661.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCAGCCAC AGATTGCCCC GGGGCTCAGC AAGATCAGCG AGGAGCCTTC AACATCCAGC 
    GACGAGAGGG CCTCACTGAT CAAGAAGGAG ATCCATGGGT CCTTGCCCCA CCTGGCCGAG
    CCCTCTGTCC CCTACCGCGG GGCTGTGTTT GCTATGGACC CCAGGAATGG CTACATGGAG
    CCTCACTATC ACCCTCCTCA TCTCTTTCCT GCATTCCATC CTCCTGTACC AATTGATGCC
    AGACACCACG AGGGCCGTTA CCATTATGAT CCATCTCCGA TCCCTCCGTT GCATGTGCCT
    TCTGCCTTAT CTAGTAGCCC CACGTATTCT GACCTGCCCT TCATCAGGAT CTCCCCACAT
    CGGAACCCTG CTGCAGCTTC CGAATCCCCC TTCAGCCCCC CGCATCCCTA CATCAACCCC
    TACATGGACT ACATCCGGTC CCTGCACAGC AGCCCATCCC TCTCCATGAT CTCGGCCGCT
    CGTGGGCTGA GCCCCACAGA TGCCCCCCAT GCCGGAGTCA GCCCAGCAGA ATACTATCAT
    CAGATGGCCC TGTTAGCCGG CCAGCGCAGC CCCTATGCAG ACATCATTCC CTCTGCTGCC
    ACCGCTGGCG CAGGGGCCAT CCACATGGAG TATCTTCACG CCATGGATAG CACCAGATTC
    CCCAGCCCTA GGCTGTCAGC CCGGCCGAGC CGAAAACGTA CACTGTCCAT ATCACCACTG
    TCCGATCATA GCTTTGACCT TCAGACCATG ATAAGGACAT CTCCCAACTC CTTGGTCACG
    ATTCTCAATA ATTCCCGCAG CAGCTCTTCA GCAAGTGGCT CCTATGGCCA CTTATCTGCA
    AGTGCAATCA GCCCTGCCCT GAGCTTCACC TACCCTTCGG CGCCCGTGTC CCTCCACATG
    CATCAGCAGA TCCTAAGCCG ACAACAGAGC TTAGGCTCAG CCTTTGGACA CAGCCCTCCG
    CTCATCCACC CCGCCCCAAC TTTCCCGACC CAGAGGCCTA TTCCCGGAAT CCCTACAGTT
    CTGAACCCCG TCCAGGTCAG CTCCGGCCCT TCCGAGTCCT CACAGAACAA GCCCACGAGT
    GAGTCTGCAG TGAGCAGTAC GGGGGACCTG GTGCACAACA AACGCTCCAA GATCAAACCA
    GATGAAGACC TCCCCAGCCC AGGGCCACGG GGCCAGCAGG AACAGCCAGA AGGAACAACC
    CTGGTGAAGG AGGAAGGGGA CAAAGATGAA AGCAAACAGG AGCCCGAAGT CATCTATGAG
    ACAAACTGCC ACTGGGAAGG CTGTTCTCGA GAGTTCGACA CCCAGGAGCA GCTGGTGCAT
    CATATAAATA ATGACCACAT CCATGGAGAG AAGAAGGAGT TTGTGTGCCG GTGGCTCGAC
    TGCTCCAGAG AACAGAAACC CTTCAAAGCC CAGTATATGC TGGTGGTGCA CATGCGCAGG
    CACACGGGGG AGAAGCCTCA CAAATGCACT TTTGAAGGTT GCACAAAGGC CTACTCGAGA
    CTCGAAAACT TGAAAACACA CTTGAGATCT CACACTGGAG AGAAACCATA CGTCTGTGAG
    CACGAAGGAT GCAACAAGGC TTTCTCTAAT GCCTCGGATC GCGCTAAGCA CCAGAACAGG
    ACACATTCCA ACGAGAAACC ATATGTGTGC AAAATCCCCG GCTGCACCAA GCGCTACACA
    GACCCCAGCT CCCTCCGGAA ACACGTGAAG ACCGTGCACG GCCCAGAGGC TCATGTCACC
    AAGAAGCAGC GAGGGGACGT GCATCCCCGG CCCCCGCCCC CTCGAGATTC CGGCAGCCAC
    TCACAGTCTA GGTCACCTGG CCGGCAGACT CAGGGAGCCC TCGGGGAGCA GAAGGACCTC
    AGCAACACTA CCTCAAAGCG GGAAGAATGC CTCCAAGTGA AAACCGTCAA GGCGGAGAAG
    CCCATGACAT CTCAGCCAAG CCCTGGTGGT CAGTCTTCAT GCAGCAGCCA ACAGTCCCCC
    ATCAGCAACT ATTCCAACAG TGGGCTCGAG CTTCCTCTGA CCGATGGAGG TGGTGTGGGC
    GACCTCAGTG CCATCGATGA AACCCCAATC ATGGACTCGA CCATTTCCAC TGCAACCACA
    GCCCTCGCTT TGCAAGCCCG GAGGAACCCA GCAGGGACCA AATGGATGGA GCACGTCAAA
    CTAGAAAGGC TCAAACAAGT GAATGGAATG CTTCCACGAC TGAACCCCAT TCTACCCCCT
    AAAGCCCCTG CAGTCTCTCC TCTCATAGGA AACGGCACGC AGCCCAGTAC CAGCTGCAGT
    CTGGGCGGGC CCATGCCCCT CCTCCCGAAC AGAAGCGACC TGTCTGGGGT GGACATCACC
    ATGTTGAACA TGCTCAACCG GAGGGACAGC AGCACCAGCA CCGTCAGCTC CGCCTACCTG
    AGCAGCCGCC GCTCCTCTGG CATCTCGCCC TGCTTCTCCA GCCGCCGGTC CAGCGAGGCG
    TCCCAGGCCG AGGGGCGACC CCAGAACGTG AGCGTGGCCG ACTCTTACGA CCCCATCTCC
    ACCGACGCCT CTCGCAGGTC CAGCGAGGCC AGCCAGGGCG ACGGTCTGCC CAGCCTGCTC
    AGCCTCACTC CCGCCCAGCA GTACCGCCTG AAAGCCAAGT ACGCTGCCGC CACGGGCGGC
    CCCCCGCCCA CCCCGCTGCC CAACATGGAG AGGATGAGCC TCAAGACCCG GATGGCCCTG
    TTGGGAGATG GCAGGGAGCC GGCGGGGACC CTGCCTCCCG TGCATCCTCC TCGGAGATGC
    AGCGACGGCG GGGCCCACGG TTACAGCCGG CGCCCACTGC TGCCCCAGGA TGCGCTGGGC
    AACGGCGTGA GAAGAGCCAG CGACCCGGTG AGGACGGTTT CGGAGAGCCT CTCTCTGCCC
    CGGGTACAGC GGTTCAACAG CCTTAACAGC TTCAACCCTC CGGTGTTGCC TCCATCCCTG
    GAAAAGCGCA ACCTCGTGCT TCAGAACTAC ACGCGGCCCG ATGGGGGCCA GGCCCGCCAC
    TTCCACTCCT CTCCCTGTCC CCCGAGCATC ACTGAGAATG TCACCCTGGA GTCCCTGACC
    ATGGACGCCG ACGTGAGCCT GAACGATGAG GACCTCCTGC CTGATGACGT GGTGCAGTAT
    TTAAATTCCC AGAACCCAGC CGGGTATGAG CAGCACTTCC CAGGCACCTT CTCTGATGAC
    AGCAAAGTGC CCCATGGCTT AGGGAGTGGA CCCGGGGACT TTGACCCACT GGGGCTGCCC
    GACAGCCACG CCAGCCACAC CGGCAGCCAG CAGTACCGCA GCCTGGAGCA GCCCTGCCCC
    GATGCCAGTA AATCCGACCT GCCCATTCAG TGGAACGAAG TCAGCTCCGG CAGCGCGGAC
    CTGTCCTCCT CCAAGCTGAA ATGTGGCCAG CGGTCCATGG GGCAGCAGGC CCGGGCCTTC
    GGGTCGTACA GCCACATGGG TGTTCACCCA CAGAATCCAT TGAGGAGTGG GGCTGGCCCA
    GCTGGGGGGT ATCCGACCCT TGGGGAGCAT GCCAGCTCCT TCGGGGGCCC GGAACACTTA
    GTGAGCCACG GTGGCGGAGC TGGCACCAAT GGGAATGCCT TCCATGAACC ACCCTATAAG
    GCCCAGCAGT ATGGGAACTG CCTCAACAGG CAGCCTGGTG CCCCCACCTT ACTCGATGGG
    GCCTGTGGCC CTGGGATCCA GGCGGCAAAG CTGAAAAGCA TCACCATGCA AGGGAGCGGG
    AGCCATCTGG ATTTTGGCCT GTCGGTGGTG CCGAATGAGT CAACCGGCAG CACGGTGAAT
    GGCATACCCA CCCCAGACCC CACAGGACAG GGGTACCTGG CTCAGCAGCT CCTCGGTGAC
    AGCACACACA CCCAGGGGGC AGGCCGCTCC GGGCAGAACC TGCTGGGGCA GGCTAGTGCT
    ACCTCACACA TCTATCAAGG GCCGGAGAGC AGCCTGCCCG GGCCTCCCAG CATCAGCGTG
    CAGCCGCCAA ACGTGGCAGC CGTCAGGGGC TACCAGCCAT GTGTTGGCTA CGGGGGCAGC
    CGGCGCCAGG CTGGGCCGAG GGGTGGTCTC GCTCTGCAGC CAGGACAGTT TAGTGACCCG
    AGTCAGACCT GCAGGGTGAA TGGCATCAAG ATGGAGATGC CAGGACAGCC CCATCAGCTC
    TGTTCGAATG TGCAGAGTTA CTCTGGTCAG TTCTATGACC AAACTGTTGG CTTCGGTCAG
    CAAGACATGA AAACTGGTTC CTTCTCCATG TCAGAAGCCA ACTGCCTGCT CCAGGGGGCC
    GGCGCCGAAA ACTCTGAATT ACTCTCCCCA GGTGCTAACC AGGTGACAAG CACAGTTGAC
    AGCCTCGACA GCCATGACCT GGAAGGGGTG CAGATTGATT TCGACGCTAT CATAGACGAC
    GGGGACCACG CCAGCCTGAT GTCAGGAGCC CTGAGCCCAA GCATTATTCA GAACCTTTCT
    CATAGCTCCT CCCGACTCAC CACGCCGCGA GCCGCCCTTC CGTTCCCGCC TCTGTCTTTG
    AGCACCACCA ACATGGCAAT CGGGGACATG AGCTCTCTGC TGACCTCCCT CGCGGAAGAA
    AGCAAATTCC TTGCGGTTAT GCAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005205718.1
    CDS270..4856
    Translation

    Target ORF information:

    RefSeq Version XM_005205661.4
    Organism Bos taurus(cattle)
    Definition Bos taurus GLI family zinc finger 3 (GLI3), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005205661.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCAGCCAC AGATTGCCCC GGGGCTCAGC AAGATCAGCG AGGAGCCTTC AACATCCAGC 
    GACGAGAGGG CCTCACTGAT CAAGAAGGAG ATCCATGGGT CCTTGCCCCA CCTGGCCGAG
    CCCTCTGTCC CCTACCGCGG GGCTGTGTTT GCTATGGACC CCAGGAATGG CTACATGGAG
    CCTCACTATC ACCCTCCTCA TCTCTTTCCT GCATTCCATC CTCCTGTACC AATTGATGCC
    AGACACCACG AGGGCCGTTA CCATTATGAT CCATCTCCGA TCCCTCCGTT GCATGTGCCT
    TCTGCCTTAT CTAGTAGCCC CACGTATTCT GACCTGCCCT TCATCAGGAT CTCCCCACAT
    CGGAACCCTG CTGCAGCTTC CGAATCCCCC TTCAGCCCCC CGCATCCCTA CATCAACCCC
    TACATGGACT ACATCCGGTC CCTGCACAGC AGCCCATCCC TCTCCATGAT CTCGGCCGCT
    CGTGGGCTGA GCCCCACAGA TGCCCCCCAT GCCGGAGTCA GCCCAGCAGA ATACTATCAT
    CAGATGGCCC TGTTAGCCGG CCAGCGCAGC CCCTATGCAG ACATCATTCC CTCTGCTGCC
    ACCGCTGGCG CAGGGGCCAT CCACATGGAG TATCTTCACG CCATGGATAG CACCAGATTC
    CCCAGCCCTA GGCTGTCAGC CCGGCCGAGC CGAAAACGTA CACTGTCCAT ATCACCACTG
    TCCGATCATA GCTTTGACCT TCAGACCATG ATAAGGACAT CTCCCAACTC CTTGGTCACG
    ATTCTCAATA ATTCCCGCAG CAGCTCTTCA GCAAGTGGCT CCTATGGCCA CTTATCTGCA
    AGTGCAATCA GCCCTGCCCT GAGCTTCACC TACCCTTCGG CGCCCGTGTC CCTCCACATG
    CATCAGCAGA TCCTAAGCCG ACAACAGAGC TTAGGCTCAG CCTTTGGACA CAGCCCTCCG
    CTCATCCACC CCGCCCCAAC TTTCCCGACC CAGAGGCCTA TTCCCGGAAT CCCTACAGTT
    CTGAACCCCG TCCAGGTCAG CTCCGGCCCT TCCGAGTCCT CACAGAACAA GCCCACGAGT
    GAGTCTGCAG TGAGCAGTAC GGGGGACCTG GTGCACAACA AACGCTCCAA GATCAAACCA
    GATGAAGACC TCCCCAGCCC AGGGCCACGG GGCCAGCAGG AACAGCCAGA AGGAACAACC
    CTGGTGAAGG AGGAAGGGGA CAAAGATGAA AGCAAACAGG AGCCCGAAGT CATCTATGAG
    ACAAACTGCC ACTGGGAAGG CTGTTCTCGA GAGTTCGACA CCCAGGAGCA GCTGGTGCAT
    CATATAAATA ATGACCACAT CCATGGAGAG AAGAAGGAGT TTGTGTGCCG GTGGCTCGAC
    TGCTCCAGAG AACAGAAACC CTTCAAAGCC CAGTATATGC TGGTGGTGCA CATGCGCAGG
    CACACGGGGG AGAAGCCTCA CAAATGCACT TTTGAAGGTT GCACAAAGGC CTACTCGAGA
    CTCGAAAACT TGAAAACACA CTTGAGATCT CACACTGGAG AGAAACCATA CGTCTGTGAG
    CACGAAGGAT GCAACAAGGC TTTCTCTAAT GCCTCGGATC GCGCTAAGCA CCAGAACAGG
    ACACATTCCA ACGAGAAACC ATATGTGTGC AAAATCCCCG GCTGCACCAA GCGCTACACA
    GACCCCAGCT CCCTCCGGAA ACACGTGAAG ACCGTGCACG GCCCAGAGGC TCATGTCACC
    AAGAAGCAGC GAGGGGACGT GCATCCCCGG CCCCCGCCCC CTCGAGATTC CGGCAGCCAC
    TCACAGTCTA GGTCACCTGG CCGGCAGACT CAGGGAGCCC TCGGGGAGCA GAAGGACCTC
    AGCAACACTA CCTCAAAGCG GGAAGAATGC CTCCAAGTGA AAACCGTCAA GGCGGAGAAG
    CCCATGACAT CTCAGCCAAG CCCTGGTGGT CAGTCTTCAT GCAGCAGCCA ACAGTCCCCC
    ATCAGCAACT ATTCCAACAG TGGGCTCGAG CTTCCTCTGA CCGATGGAGG TGGTGTGGGC
    GACCTCAGTG CCATCGATGA AACCCCAATC ATGGACTCGA CCATTTCCAC TGCAACCACA
    GCCCTCGCTT TGCAAGCCCG GAGGAACCCA GCAGGGACCA AATGGATGGA GCACGTCAAA
    CTAGAAAGGC TCAAACAAGT GAATGGAATG CTTCCACGAC TGAACCCCAT TCTACCCCCT
    AAAGCCCCTG CAGTCTCTCC TCTCATAGGA AACGGCACGC AGCCCAGTAC CAGCTGCAGT
    CTGGGCGGGC CCATGCCCCT CCTCCCGAAC AGAAGCGACC TGTCTGGGGT GGACATCACC
    ATGTTGAACA TGCTCAACCG GAGGGACAGC AGCACCAGCA CCGTCAGCTC CGCCTACCTG
    AGCAGCCGCC GCTCCTCTGG CATCTCGCCC TGCTTCTCCA GCCGCCGGTC CAGCGAGGCG
    TCCCAGGCCG AGGGGCGACC CCAGAACGTG AGCGTGGCCG ACTCTTACGA CCCCATCTCC
    ACCGACGCCT CTCGCAGGTC CAGCGAGGCC AGCCAGGGCG ACGGTCTGCC CAGCCTGCTC
    AGCCTCACTC CCGCCCAGCA GTACCGCCTG AAAGCCAAGT ACGCTGCCGC CACGGGCGGC
    CCCCCGCCCA CCCCGCTGCC CAACATGGAG AGGATGAGCC TCAAGACCCG GATGGCCCTG
    TTGGGAGATG GCAGGGAGCC GGCGGGGACC CTGCCTCCCG TGCATCCTCC TCGGAGATGC
    AGCGACGGCG GGGCCCACGG TTACAGCCGG CGCCCACTGC TGCCCCAGGA TGCGCTGGGC
    AACGGCGTGA GAAGAGCCAG CGACCCGGTG AGGACGGTTT CGGAGAGCCT CTCTCTGCCC
    CGGGTACAGC GGTTCAACAG CCTTAACAGC TTCAACCCTC CGGTGTTGCC TCCATCCCTG
    GAAAAGCGCA ACCTCGTGCT TCAGAACTAC ACGCGGCCCG ATGGGGGCCA GGCCCGCCAC
    TTCCACTCCT CTCCCTGTCC CCCGAGCATC ACTGAGAATG TCACCCTGGA GTCCCTGACC
    ATGGACGCCG ACGTGAGCCT GAACGATGAG GACCTCCTGC CTGATGACGT GGTGCAGTAT
    TTAAATTCCC AGAACCCAGC CGGGTATGAG CAGCACTTCC CAGGCACCTT CTCTGATGAC
    AGCAAAGTGC CCCATGGCTT AGGGAGTGGA CCCGGGGACT TTGACCCACT GGGGCTGCCC
    GACAGCCACG CCAGCCACAC CGGCAGCCAG CAGTACCGCA GCCTGGAGCA GCCCTGCCCC
    GATGCCAGTA AATCCGACCT GCCCATTCAG TGGAACGAAG TCAGCTCCGG CAGCGCGGAC
    CTGTCCTCCT CCAAGCTGAA ATGTGGCCAG CGGTCCATGG GGCAGCAGGC CCGGGCCTTC
    GGGTCGTACA GCCACATGGG TGTTCACCCA CAGAATCCAT TGAGGAGTGG GGCTGGCCCA
    GCTGGGGGGT ATCCGACCCT TGGGGAGCAT GCCAGCTCCT TCGGGGGCCC GGAACACTTA
    GTGAGCCACG GTGGCGGAGC TGGCACCAAT GGGAATGCCT TCCATGAACC ACCCTATAAG
    GCCCAGCAGT ATGGGAACTG CCTCAACAGG CAGCCTGGTG CCCCCACCTT ACTCGATGGG
    GCCTGTGGCC CTGGGATCCA GGCGGCAAAG CTGAAAAGCA TCACCATGCA AGGGAGCGGG
    AGCCATCTGG ATTTTGGCCT GTCGGTGGTG CCGAATGAGT CAACCGGCAG CACGGTGAAT
    GGCATACCCA CCCCAGACCC CACAGGACAG GGGTACCTGG CTCAGCAGCT CCTCGGTGAC
    AGCACACACA CCCAGGGGGC AGGCCGCTCC GGGCAGAACC TGCTGGGGCA GGCTAGTGCT
    ACCTCACACA TCTATCAAGG GCCGGAGAGC AGCCTGCCCG GGCCTCCCAG CATCAGCGTG
    CAGCCGCCAA ACGTGGCAGC CGTCAGGGGC TACCAGCCAT GTGTTGGCTA CGGGGGCAGC
    CGGCGCCAGG CTGGGCCGAG GGGTGGTCTC GCTCTGCAGC CAGGACAGTT TAGTGACCCG
    AGTCAGACCT GCAGGGTGAA TGGCATCAAG ATGGAGATGC CAGGACAGCC CCATCAGCTC
    TGTTCGAATG TGCAGAGTTA CTCTGGTCAG TTCTATGACC AAACTGTTGG CTTCGGTCAG
    CAAGACATGA AAACTGGTTC CTTCTCCATG TCAGAAGCCA ACTGCCTGCT CCAGGGGGCC
    GGCGCCGAAA ACTCTGAATT ACTCTCCCCA GGTGCTAACC AGGTGACAAG CACAGTTGAC
    AGCCTCGACA GCCATGACCT GGAAGGGGTG CAGATTGATT TCGACGCTAT CATAGACGAC
    GGGGACCACG CCAGCCTGAT GTCAGGAGCC CTGAGCCCAA GCATTATTCA GAACCTTTCT
    CATAGCTCCT CCCGACTCAC CACGCCGCGA GCCGCCCTTC CGTTCCCGCC TCTGTCTTTG
    AGCACCACCA ACATGGCAAT CGGGGACATG AGCTCTCTGC TGACCTCCCT CGCGGAAGAA
    AGCAAATTCC TTGCGGTTAT GCAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa18737
    Clone ID Related Accession (Same CDS sequence) XM_015463085.1 , XM_005205659.3 , XM_005205659.4
    Accession Version XM_015463085.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4764bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product transcriptional activator GLI3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644732.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGAGGCCC AGTCCCAGAG CTCGACGACC ACTGAGAAAA AGAAAGTGGA GAATTCCATA 
    GTGAAATGCC CCACTCGGCC AGATGTCAGC GAGAAAGCCG TGGCCTCCAG CACCACTTCC
    AATGAGGATG AAAGTCCTGG ACAGACCTAT CACAGAGAGA GAAGAAACGC AGTCACTATG
    CAGCCACAGA TTGCCCCGGG GCTCAGCAAG ATCAGCGAGG AGCCTTCAAC ATCCAGCGAC
    GAGAGGGCCT CACTGATCAA GAAGGAGATC CATGGGTCCT TGCCCCACCT GGCCGAGCCC
    TCTGTCCCCT ACCGCGGGGC TGTGTTTGCT ATGGACCCCA GGAATGGCTA CATGGAGCCT
    CACTATCACC CTCCTCATCT CTTTCCTGCA TTCCATCCTC CTGTACCAAT TGATGCCAGA
    CACCACGAGG GCCGTTACCA TTATGATCCA TCTCCGATCC CTCCGTTGCA TGTGCCTTCT
    GCCTTATCTA GTAGCCCCAC GTATTCTGAC CTGCCCTTCA TCAGGATCTC CCCACATCGG
    AACCCTGCTG CAGCTTCCGA ATCCCCCTTC AGCCCCCCGC ATCCCTACAT CAACCCCTAC
    ATGGACTACA TCCGGTCCCT GCACAGCAGC CCATCCCTCT CCATGATCTC GGCCGCTCGT
    GGGCTGAGCC CCACAGATGC CCCCCATGCC GGAGTCAGCC CAGCAGAATA CTATCATCAG
    ATGGCCCTGT TAGCCGGCCA GCGCAGCCCC TATGCAGACA TCATTCCCTC TGCTGCCACC
    GCTGGCGCAG GGGCCATCCA CATGGAGTAT CTTCACGCCA TGGATAGCAC CAGATTCCCC
    AGCCCTAGGC TGTCAGCCCG GCCGAGCCGA AAACGTACAC TGTCCATATC ACCACTGTCC
    GATCATAGCT TTGACCTTCA GACCATGATA AGGACATCTC CCAACTCCTT GGTCACGATT
    CTCAATAATT CCCGCAGCAG CTCTTCAGCA AGTGGCTCCT ATGGCCACTT ATCTGCAAGT
    GCAATCAGCC CTGCCCTGAG CTTCACCTAC CCTTCGGCGC CCGTGTCCCT CCACATGCAT
    CAGCAGATCC TAAGCCGACA ACAGAGCTTA GGCTCAGCCT TTGGACACAG CCCTCCGCTC
    ATCCACCCCG CCCCAACTTT CCCGACCCAG AGGCCTATTC CCGGAATCCC TACAGTTCTG
    AACCCCGTCC AGGTCAGCTC CGGCCCTTCC GAGTCCTCAC AGAACAAGCC CACGAGTGAG
    TCTGCAGTGA GCAGTACGGG GGACCTGGTG CACAACAAAC GCTCCAAGAT CAAACCAGAT
    GAAGACCTCC CCAGCCCAGG GCCACGGGGC CAGCAGGAAC AGCCAGAAGG AACAACCCTG
    GTGAAGGAGG AAGGGGACAA AGATGAAAGC AAACAGGAGC CCGAAGTCAT CTATGAGACA
    AACTGCCACT GGGAAGGCTG TTCTCGAGAG TTCGACACCC AGGAGCAGCT GGTGCATCAT
    ATAAATAATG ACCACATCCA TGGAGAGAAG AAGGAGTTTG TGTGCCGGTG GCTCGACTGC
    TCCAGAGAAC AGAAACCCTT CAAAGCCCAG TATATGCTGG TGGTGCACAT GCGCAGGCAC
    ACGGGGGAGA AGCCTCACAA ATGCACTTTT GAAGGTTGCA CAAAGGCCTA CTCGAGACTC
    GAAAACTTGA AAACACACTT GAGATCTCAC ACTGGAGAGA AACCATACGT CTGTGAGCAC
    GAAGGATGCA ACAAGGCTTT CTCTAATGCC TCGGATCGCG CTAAGCACCA GAACAGGACA
    CATTCCAACG AGAAACCATA TGTGTGCAAA ATCCCCGGCT GCACCAAGCG CTACACAGAC
    CCCAGCTCCC TCCGGAAACA CGTGAAGACC GTGCACGGCC CAGAGGCTCA TGTCACCAAG
    AAGCAGCGAG GGGACGTGCA TCCCCGGCCC CCGCCCCCTC GAGATTCCGG CAGCCACTCA
    CAGTCTAGGT CACCTGGCCG GCAGACTCAG GGAGCCCTCG GGGAGCAGAA GGACCTCAGC
    AACACTACCT CAAAGCGGGA AGAATGCCTC CAAGTGAAAA CCGTCAAGGC GGAGAAGCCC
    ATGACATCTC AGCCAAGCCC TGGTGGTCAG TCTTCATGCA GCAGCCAACA GTCCCCCATC
    AGCAACTATT CCAACAGTGG GCTCGAGCTT CCTCTGACCG ATGGAGGTGG TGTGGGCGAC
    CTCAGTGCCA TCGATGAAAC CCCAATCATG GACTCGACCA TTTCCACTGC AACCACAGCC
    CTCGCTTTGC AAGCCCGGAG GAACCCAGCA GGGACCAAAT GGATGGAGCA CGTCAAACTA
    GAAAGGCTCA AACAAGTGAA TGGAATGCTT CCACGACTGA ACCCCATTCT ACCCCCTAAA
    GCCCCTGCAG TCTCTCCTCT CATAGGAAAC GGCACGCAGC CCAGTACCAG CTGCAGTCTG
    GGCGGGCCCA TGCCCCTCCT CCCGAACAGA AGCGACCTGT CTGGGGTGGA CATCACCATG
    TTGAACATGC TCAACCGGAG GGACAGCAGC ACCAGCACCG TCAGCTCCGC CTACCTGAGC
    AGCCGCCGCT CCTCTGGCAT CTCGCCCTGC TTCTCCAGCC GCCGGTCCAG CGAGGCGTCC
    CAGGCCGAGG GGCGACCCCA GAACGTGAGC GTGGCCGACT CTTACGACCC CATCTCCACC
    GACGCCTCTC GCAGGTCCAG CGAGGCCAGC CAGGGCGACG GTCTGCCCAG CCTGCTCAGC
    CTCACTCCCG CCCAGCAGTA CCGCCTGAAA GCCAAGTACG CTGCCGCCAC GGGCGGCCCC
    CCGCCCACCC CGCTGCCCAA CATGGAGAGG ATGAGCCTCA AGACCCGGAT GGCCCTGTTG
    GGAGATGGCA GGGAGCCGGC GGGGACCCTG CCTCCCGTGC ATCCTCCTCG GAGATGCAGC
    GACGGCGGGG CCCACGGTTA CAGCCGGCGC CCACTGCTGC CCCAGGATGC GCTGGGCAAC
    GGCGTGAGAA GAGCCAGCGA CCCGGTGAGG ACGGTTTCGG AGAGCCTCTC TCTGCCCCGG
    GTACAGCGGT TCAACAGCCT TAACAGCTTC AACCCTCCGG TGTTGCCTCC ATCCCTGGAA
    AAGCGCAACC TCGTGCTTCA GAACTACACG CGGCCCGATG GGGGCCAGGC CCGCCACTTC
    CACTCCTCTC CCTGTCCCCC GAGCATCACT GAGAATGTCA CCCTGGAGTC CCTGACCATG
    GACGCCGACG TGAGCCTGAA CGATGAGGAC CTCCTGCCTG ATGACGTGGT GCAGTATTTA
    AATTCCCAGA ACCCAGCCGG GTATGAGCAG CACTTCCCAG GCACCTTCTC TGATGACAGC
    AAAGTGCCCC ATGGCTTAGG GAGTGGACCC GGGGACTTTG ACCCACTGGG GCTGCCCGAC
    AGCCACGCCA GCCACACCGG CAGCCAGCAG TACCGCAGCC TGGAGCAGCC CTGCCCCGAT
    GCCAGTAAAT CCGACCTGCC CATTCAGTGG AACGAAGTCA GCTCCGGCAG CGCGGACCTG
    TCCTCCTCCA AGCTGAAATG TGGCCAGCGG TCCATGGGGC AGCAGGCCCG GGCCTTCGGG
    TCGTACAGCC ACATGGGTGT TCACCCACAG AATCCATTGA GGAGTGGGGC TGGCCCAGCT
    GGGGGGTATC CGACCCTTGG GGAGCATGCC AGCTCCTTCG GGGGCCCGGA ACACTTAGTG
    AGCCACGGTG GCGGAGCTGG CACCAATGGG AATGCCTTCC ATGAACCACC CTATAAGGCC
    CAGCAGTATG GGAACTGCCT CAACAGGCAG CCTGGTGCCC CCACCTTACT CGATGGGGCC
    TGTGGCCCTG GGATCCAGGC GGCAAAGCTG AAAAGCATCA CCATGCAAGG GAGCGGGAGC
    CATCTGGATT TTGGCCTGTC GGTGGTGCCG AATGAGTCAA CCGGCAGCAC GGTGAATGGC
    ATACCCACCC CAGACCCCAC AGGACAGGGG TACCTGGCTC AGCAGCTCCT CGGTGACAGC
    ACACACACCC AGGGGGCAGG CCGCTCCGGG CAGAACCTGC TGGGGCAGGC TAGTGCTACC
    TCACACATCT ATCAAGGGCC GGAGAGCAGC CTGCCCGGGC CTCCCAGCAT CAGCGTGCAG
    CCGCCAAACG TGGCAGCCGT CAGGGGCTAC CAGCCATGTG TTGGCTACGG GGGCAGCCGG
    CGCCAGGCTG GGCCGAGGGG TGGTCTCGCT CTGCAGCCAG GACAGTTTAG TGACCCGAGT
    CAGACCTGCA GGGTGAATGG CATCAAGATG GAGATGCCAG GACAGCCCCA TCAGCTCTGT
    TCGAATGTGC AGAGTTACTC TGGTCAGTTC TATGACCAAA CTGTTGGCTT CGGTCAGCAA
    GACATGAAAA CTGGTTCCTT CTCCATGTCA GAAGCCAACT GCCTGCTCCA GGGGGCCGGC
    GCCGAAAACT CTGAATTACT CTCCCCAGGT GCTAACCAGG TGACAAGCAC AGTTGACAGC
    CTCGACAGCC ATGACCTGGA AGGGGTGCAG ATTGATTTCG ACGCTATCAT AGACGACGGG
    GACCACGCCA GCCTGATGTC AGGAGCCCTG AGCCCAAGCA TTATTCAGAA CCTTTCTCAT
    AGCTCCTCCC GACTCACCAC GCCGCGAGCC GCCCTTCCGT TCCCGCCTCT GTCTTTGAGC
    ACCACCAACA TGGCAATCGG GGACATGAGC TCTCTGCTGA CCTCCCTCGC GGAAGAAAGC
    AAATTCCTTG CGGTTATGCA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015318571.1
    CDS229..4992
    Translation

    Target ORF information:

    RefSeq Version XM_015463085.1
    Organism Bos taurus(cattle)
    Definition Bos taurus GLI family zinc finger 3 (GLI3), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463085.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGAGGCCC AGTCCCAGAG CTCGACGACC ACTGAGAAAA AGAAAGTGGA GAATTCCATA 
    GTGAAATGCC CCACTCGGCC AGATGTCAGC GAGAAAGCCG TGGCCTCCAG CACCACTTCC
    AATGAGGATG AAAGTCCTGG ACAGACCTAT CACAGAGAGA GAAGAAACGC AGTCACTATG
    CAGCCACAGA TTGCCCCGGG GCTCAGCAAG ATCAGCGAGG AGCCTTCAAC ATCCAGCGAC
    GAGAGGGCCT CACTGATCAA GAAGGAGATC CATGGGTCCT TGCCCCACCT GGCCGAGCCC
    TCTGTCCCCT ACCGCGGGGC TGTGTTTGCT ATGGACCCCA GGAATGGCTA CATGGAGCCT
    CACTATCACC CTCCTCATCT CTTTCCTGCA TTCCATCCTC CTGTACCAAT TGATGCCAGA
    CACCACGAGG GCCGTTACCA TTATGATCCA TCTCCGATCC CTCCGTTGCA TGTGCCTTCT
    GCCTTATCTA GTAGCCCCAC GTATTCTGAC CTGCCCTTCA TCAGGATCTC CCCACATCGG
    AACCCTGCTG CAGCTTCCGA ATCCCCCTTC AGCCCCCCGC ATCCCTACAT CAACCCCTAC
    ATGGACTACA TCCGGTCCCT GCACAGCAGC CCATCCCTCT CCATGATCTC GGCCGCTCGT
    GGGCTGAGCC CCACAGATGC CCCCCATGCC GGAGTCAGCC CAGCAGAATA CTATCATCAG
    ATGGCCCTGT TAGCCGGCCA GCGCAGCCCC TATGCAGACA TCATTCCCTC TGCTGCCACC
    GCTGGCGCAG GGGCCATCCA CATGGAGTAT CTTCACGCCA TGGATAGCAC CAGATTCCCC
    AGCCCTAGGC TGTCAGCCCG GCCGAGCCGA AAACGTACAC TGTCCATATC ACCACTGTCC
    GATCATAGCT TTGACCTTCA GACCATGATA AGGACATCTC CCAACTCCTT GGTCACGATT
    CTCAATAATT CCCGCAGCAG CTCTTCAGCA AGTGGCTCCT ATGGCCACTT ATCTGCAAGT
    GCAATCAGCC CTGCCCTGAG CTTCACCTAC CCTTCGGCGC CCGTGTCCCT CCACATGCAT
    CAGCAGATCC TAAGCCGACA ACAGAGCTTA GGCTCAGCCT TTGGACACAG CCCTCCGCTC
    ATCCACCCCG CCCCAACTTT CCCGACCCAG AGGCCTATTC CCGGAATCCC TACAGTTCTG
    AACCCCGTCC AGGTCAGCTC CGGCCCTTCC GAGTCCTCAC AGAACAAGCC CACGAGTGAG
    TCTGCAGTGA GCAGTACGGG GGACCTGGTG CACAACAAAC GCTCCAAGAT CAAACCAGAT
    GAAGACCTCC CCAGCCCAGG GCCACGGGGC CAGCAGGAAC AGCCAGAAGG AACAACCCTG
    GTGAAGGAGG AAGGGGACAA AGATGAAAGC AAACAGGAGC CCGAAGTCAT CTATGAGACA
    AACTGCCACT GGGAAGGCTG TTCTCGAGAG TTCGACACCC AGGAGCAGCT GGTGCATCAT
    ATAAATAATG ACCACATCCA TGGAGAGAAG AAGGAGTTTG TGTGCCGGTG GCTCGACTGC
    TCCAGAGAAC AGAAACCCTT CAAAGCCCAG TATATGCTGG TGGTGCACAT GCGCAGGCAC
    ACGGGGGAGA AGCCTCACAA ATGCACTTTT GAAGGTTGCA CAAAGGCCTA CTCGAGACTC
    GAAAACTTGA AAACACACTT GAGATCTCAC ACTGGAGAGA AACCATACGT CTGTGAGCAC
    GAAGGATGCA ACAAGGCTTT CTCTAATGCC TCGGATCGCG CTAAGCACCA GAACAGGACA
    CATTCCAACG AGAAACCATA TGTGTGCAAA ATCCCCGGCT GCACCAAGCG CTACACAGAC
    CCCAGCTCCC TCCGGAAACA CGTGAAGACC GTGCACGGCC CAGAGGCTCA TGTCACCAAG
    AAGCAGCGAG GGGACGTGCA TCCCCGGCCC CCGCCCCCTC GAGATTCCGG CAGCCACTCA
    CAGTCTAGGT CACCTGGCCG GCAGACTCAG GGAGCCCTCG GGGAGCAGAA GGACCTCAGC
    AACACTACCT CAAAGCGGGA AGAATGCCTC CAAGTGAAAA CCGTCAAGGC GGAGAAGCCC
    ATGACATCTC AGCCAAGCCC TGGTGGTCAG TCTTCATGCA GCAGCCAACA GTCCCCCATC
    AGCAACTATT CCAACAGTGG GCTCGAGCTT CCTCTGACCG ATGGAGGTGG TGTGGGCGAC
    CTCAGTGCCA TCGATGAAAC CCCAATCATG GACTCGACCA TTTCCACTGC AACCACAGCC
    CTCGCTTTGC AAGCCCGGAG GAACCCAGCA GGGACCAAAT GGATGGAGCA CGTCAAACTA
    GAAAGGCTCA AACAAGTGAA TGGAATGCTT CCACGACTGA ACCCCATTCT ACCCCCTAAA
    GCCCCTGCAG TCTCTCCTCT CATAGGAAAC GGCACGCAGC CCAGTACCAG CTGCAGTCTG
    GGCGGGCCCA TGCCCCTCCT CCCGAACAGA AGCGACCTGT CTGGGGTGGA CATCACCATG
    TTGAACATGC TCAACCGGAG GGACAGCAGC ACCAGCACCG TCAGCTCCGC CTACCTGAGC
    AGCCGCCGCT CCTCTGGCAT CTCGCCCTGC TTCTCCAGCC GCCGGTCCAG CGAGGCGTCC
    CAGGCCGAGG GGCGACCCCA GAACGTGAGC GTGGCCGACT CTTACGACCC CATCTCCACC
    GACGCCTCTC GCAGGTCCAG CGAGGCCAGC CAGGGCGACG GTCTGCCCAG CCTGCTCAGC
    CTCACTCCCG CCCAGCAGTA CCGCCTGAAA GCCAAGTACG CTGCCGCCAC GGGCGGCCCC
    CCGCCCACCC CGCTGCCCAA CATGGAGAGG ATGAGCCTCA AGACCCGGAT GGCCCTGTTG
    GGAGATGGCA GGGAGCCGGC GGGGACCCTG CCTCCCGTGC ATCCTCCTCG GAGATGCAGC
    GACGGCGGGG CCCACGGTTA CAGCCGGCGC CCACTGCTGC CCCAGGATGC GCTGGGCAAC
    GGCGTGAGAA GAGCCAGCGA CCCGGTGAGG ACGGTTTCGG AGAGCCTCTC TCTGCCCCGG
    GTACAGCGGT TCAACAGCCT TAACAGCTTC AACCCTCCGG TGTTGCCTCC ATCCCTGGAA
    AAGCGCAACC TCGTGCTTCA GAACTACACG CGGCCCGATG GGGGCCAGGC CCGCCACTTC
    CACTCCTCTC CCTGTCCCCC GAGCATCACT GAGAATGTCA CCCTGGAGTC CCTGACCATG
    GACGCCGACG TGAGCCTGAA CGATGAGGAC CTCCTGCCTG ATGACGTGGT GCAGTATTTA
    AATTCCCAGA ACCCAGCCGG GTATGAGCAG CACTTCCCAG GCACCTTCTC TGATGACAGC
    AAAGTGCCCC ATGGCTTAGG GAGTGGACCC GGGGACTTTG ACCCACTGGG GCTGCCCGAC
    AGCCACGCCA GCCACACCGG CAGCCAGCAG TACCGCAGCC TGGAGCAGCC CTGCCCCGAT
    GCCAGTAAAT CCGACCTGCC CATTCAGTGG AACGAAGTCA GCTCCGGCAG CGCGGACCTG
    TCCTCCTCCA AGCTGAAATG TGGCCAGCGG TCCATGGGGC AGCAGGCCCG GGCCTTCGGG
    TCGTACAGCC ACATGGGTGT TCACCCACAG AATCCATTGA GGAGTGGGGC TGGCCCAGCT
    GGGGGGTATC CGACCCTTGG GGAGCATGCC AGCTCCTTCG GGGGCCCGGA ACACTTAGTG
    AGCCACGGTG GCGGAGCTGG CACCAATGGG AATGCCTTCC ATGAACCACC CTATAAGGCC
    CAGCAGTATG GGAACTGCCT CAACAGGCAG CCTGGTGCCC CCACCTTACT CGATGGGGCC
    TGTGGCCCTG GGATCCAGGC GGCAAAGCTG AAAAGCATCA CCATGCAAGG GAGCGGGAGC
    CATCTGGATT TTGGCCTGTC GGTGGTGCCG AATGAGTCAA CCGGCAGCAC GGTGAATGGC
    ATACCCACCC CAGACCCCAC AGGACAGGGG TACCTGGCTC AGCAGCTCCT CGGTGACAGC
    ACACACACCC AGGGGGCAGG CCGCTCCGGG CAGAACCTGC TGGGGCAGGC TAGTGCTACC
    TCACACATCT ATCAAGGGCC GGAGAGCAGC CTGCCCGGGC CTCCCAGCAT CAGCGTGCAG
    CCGCCAAACG TGGCAGCCGT CAGGGGCTAC CAGCCATGTG TTGGCTACGG GGGCAGCCGG
    CGCCAGGCTG GGCCGAGGGG TGGTCTCGCT CTGCAGCCAG GACAGTTTAG TGACCCGAGT
    CAGACCTGCA GGGTGAATGG CATCAAGATG GAGATGCCAG GACAGCCCCA TCAGCTCTGT
    TCGAATGTGC AGAGTTACTC TGGTCAGTTC TATGACCAAA CTGTTGGCTT CGGTCAGCAA
    GACATGAAAA CTGGTTCCTT CTCCATGTCA GAAGCCAACT GCCTGCTCCA GGGGGCCGGC
    GCCGAAAACT CTGAATTACT CTCCCCAGGT GCTAACCAGG TGACAAGCAC AGTTGACAGC
    CTCGACAGCC ATGACCTGGA AGGGGTGCAG ATTGATTTCG ACGCTATCAT AGACGACGGG
    GACCACGCCA GCCTGATGTC AGGAGCCCTG AGCCCAAGCA TTATTCAGAA CCTTTCTCAT
    AGCTCCTCCC GACTCACCAC GCCGCGAGCC GCCCTTCCGT TCCCGCCTCT GTCTTTGAGC
    ACCACCAACA TGGCAATCGG GGACATGAGC TCTCTGCTGA CCTCCCTCGC GGAAGAAAGC
    AAATTCCTTG CGGTTATGCA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa18737
    Clone ID Related Accession (Same CDS sequence) XM_015463085.1 , XM_005205659.3 , XM_005205659.4
    Accession Version XM_005205659.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4764bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product transcriptional activator GLI3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103903.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005205659.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGAGGCCC AGTCCCAGAG CTCGACGACC ACTGAGAAAA AGAAAGTGGA GAATTCCATA 
    GTGAAATGCC CCACTCGGCC AGATGTCAGC GAGAAAGCCG TGGCCTCCAG CACCACTTCC
    AATGAGGATG AAAGTCCTGG ACAGACCTAT CACAGAGAGA GAAGAAACGC AGTCACTATG
    CAGCCACAGA TTGCCCCGGG GCTCAGCAAG ATCAGCGAGG AGCCTTCAAC ATCCAGCGAC
    GAGAGGGCCT CACTGATCAA GAAGGAGATC CATGGGTCCT TGCCCCACCT GGCCGAGCCC
    TCTGTCCCCT ACCGCGGGGC TGTGTTTGCT ATGGACCCCA GGAATGGCTA CATGGAGCCT
    CACTATCACC CTCCTCATCT CTTTCCTGCA TTCCATCCTC CTGTACCAAT TGATGCCAGA
    CACCACGAGG GCCGTTACCA TTATGATCCA TCTCCGATCC CTCCGTTGCA TGTGCCTTCT
    GCCTTATCTA GTAGCCCCAC GTATTCTGAC CTGCCCTTCA TCAGGATCTC CCCACATCGG
    AACCCTGCTG CAGCTTCCGA ATCCCCCTTC AGCCCCCCGC ATCCCTACAT CAACCCCTAC
    ATGGACTACA TCCGGTCCCT GCACAGCAGC CCATCCCTCT CCATGATCTC GGCCGCTCGT
    GGGCTGAGCC CCACAGATGC CCCCCATGCC GGAGTCAGCC CAGCAGAATA CTATCATCAG
    ATGGCCCTGT TAGCCGGCCA GCGCAGCCCC TATGCAGACA TCATTCCCTC TGCTGCCACC
    GCTGGCGCAG GGGCCATCCA CATGGAGTAT CTTCACGCCA TGGATAGCAC CAGATTCCCC
    AGCCCTAGGC TGTCAGCCCG GCCGAGCCGA AAACGTACAC TGTCCATATC ACCACTGTCC
    GATCATAGCT TTGACCTTCA GACCATGATA AGGACATCTC CCAACTCCTT GGTCACGATT
    CTCAATAATT CCCGCAGCAG CTCTTCAGCA AGTGGCTCCT ATGGCCACTT ATCTGCAAGT
    GCAATCAGCC CTGCCCTGAG CTTCACCTAC CCTTCGGCGC CCGTGTCCCT CCACATGCAT
    CAGCAGATCC TAAGCCGACA ACAGAGCTTA GGCTCAGCCT TTGGACACAG CCCTCCGCTC
    ATCCACCCCG CCCCAACTTT CCCGACCCAG AGGCCTATTC CCGGAATCCC TACAGTTCTG
    AACCCCGTCC AGGTCAGCTC CGGCCCTTCC GAGTCCTCAC AGAACAAGCC CACGAGTGAG
    TCTGCAGTGA GCAGTACGGG GGACCTGGTG CACAACAAAC GCTCCAAGAT CAAACCAGAT
    GAAGACCTCC CCAGCCCAGG GCCACGGGGC CAGCAGGAAC AGCCAGAAGG AACAACCCTG
    GTGAAGGAGG AAGGGGACAA AGATGAAAGC AAACAGGAGC CCGAAGTCAT CTATGAGACA
    AACTGCCACT GGGAAGGCTG TTCTCGAGAG TTCGACACCC AGGAGCAGCT GGTGCATCAT
    ATAAATAATG ACCACATCCA TGGAGAGAAG AAGGAGTTTG TGTGCCGGTG GCTCGACTGC
    TCCAGAGAAC AGAAACCCTT CAAAGCCCAG TATATGCTGG TGGTGCACAT GCGCAGGCAC
    ACGGGGGAGA AGCCTCACAA ATGCACTTTT GAAGGTTGCA CAAAGGCCTA CTCGAGACTC
    GAAAACTTGA AAACACACTT GAGATCTCAC ACTGGAGAGA AACCATACGT CTGTGAGCAC
    GAAGGATGCA ACAAGGCTTT CTCTAATGCC TCGGATCGCG CTAAGCACCA GAACAGGACA
    CATTCCAACG AGAAACCATA TGTGTGCAAA ATCCCCGGCT GCACCAAGCG CTACACAGAC
    CCCAGCTCCC TCCGGAAACA CGTGAAGACC GTGCACGGCC CAGAGGCTCA TGTCACCAAG
    AAGCAGCGAG GGGACGTGCA TCCCCGGCCC CCGCCCCCTC GAGATTCCGG CAGCCACTCA
    CAGTCTAGGT CACCTGGCCG GCAGACTCAG GGAGCCCTCG GGGAGCAGAA GGACCTCAGC
    AACACTACCT CAAAGCGGGA AGAATGCCTC CAAGTGAAAA CCGTCAAGGC GGAGAAGCCC
    ATGACATCTC AGCCAAGCCC TGGTGGTCAG TCTTCATGCA GCAGCCAACA GTCCCCCATC
    AGCAACTATT CCAACAGTGG GCTCGAGCTT CCTCTGACCG ATGGAGGTGG TGTGGGCGAC
    CTCAGTGCCA TCGATGAAAC CCCAATCATG GACTCGACCA TTTCCACTGC AACCACAGCC
    CTCGCTTTGC AAGCCCGGAG GAACCCAGCA GGGACCAAAT GGATGGAGCA CGTCAAACTA
    GAAAGGCTCA AACAAGTGAA TGGAATGCTT CCACGACTGA ACCCCATTCT ACCCCCTAAA
    GCCCCTGCAG TCTCTCCTCT CATAGGAAAC GGCACGCAGC CCAGTACCAG CTGCAGTCTG
    GGCGGGCCCA TGCCCCTCCT CCCGAACAGA AGCGACCTGT CTGGGGTGGA CATCACCATG
    TTGAACATGC TCAACCGGAG GGACAGCAGC ACCAGCACCG TCAGCTCCGC CTACCTGAGC
    AGCCGCCGCT CCTCTGGCAT CTCGCCCTGC TTCTCCAGCC GCCGGTCCAG CGAGGCGTCC
    CAGGCCGAGG GGCGACCCCA GAACGTGAGC GTGGCCGACT CTTACGACCC CATCTCCACC
    GACGCCTCTC GCAGGTCCAG CGAGGCCAGC CAGGGCGACG GTCTGCCCAG CCTGCTCAGC
    CTCACTCCCG CCCAGCAGTA CCGCCTGAAA GCCAAGTACG CTGCCGCCAC GGGCGGCCCC
    CCGCCCACCC CGCTGCCCAA CATGGAGAGG ATGAGCCTCA AGACCCGGAT GGCCCTGTTG
    GGAGATGGCA GGGAGCCGGC GGGGACCCTG CCTCCCGTGC ATCCTCCTCG GAGATGCAGC
    GACGGCGGGG CCCACGGTTA CAGCCGGCGC CCACTGCTGC CCCAGGATGC GCTGGGCAAC
    GGCGTGAGAA GAGCCAGCGA CCCGGTGAGG ACGGTTTCGG AGAGCCTCTC TCTGCCCCGG
    GTACAGCGGT TCAACAGCCT TAACAGCTTC AACCCTCCGG TGTTGCCTCC ATCCCTGGAA
    AAGCGCAACC TCGTGCTTCA GAACTACACG CGGCCCGATG GGGGCCAGGC CCGCCACTTC
    CACTCCTCTC CCTGTCCCCC GAGCATCACT GAGAATGTCA CCCTGGAGTC CCTGACCATG
    GACGCCGACG TGAGCCTGAA CGATGAGGAC CTCCTGCCTG ATGACGTGGT GCAGTATTTA
    AATTCCCAGA ACCCAGCCGG GTATGAGCAG CACTTCCCAG GCACCTTCTC TGATGACAGC
    AAAGTGCCCC ATGGCTTAGG GAGTGGACCC GGGGACTTTG ACCCACTGGG GCTGCCCGAC
    AGCCACGCCA GCCACACCGG CAGCCAGCAG TACCGCAGCC TGGAGCAGCC CTGCCCCGAT
    GCCAGTAAAT CCGACCTGCC CATTCAGTGG AACGAAGTCA GCTCCGGCAG CGCGGACCTG
    TCCTCCTCCA AGCTGAAATG TGGCCAGCGG TCCATGGGGC AGCAGGCCCG GGCCTTCGGG
    TCGTACAGCC ACATGGGTGT TCACCCACAG AATCCATTGA GGAGTGGGGC TGGCCCAGCT
    GGGGGGTATC CGACCCTTGG GGAGCATGCC AGCTCCTTCG GGGGCCCGGA ACACTTAGTG
    AGCCACGGTG GCGGAGCTGG CACCAATGGG AATGCCTTCC ATGAACCACC CTATAAGGCC
    CAGCAGTATG GGAACTGCCT CAACAGGCAG CCTGGTGCCC CCACCTTACT CGATGGGGCC
    TGTGGCCCTG GGATCCAGGC GGCAAAGCTG AAAAGCATCA CCATGCAAGG GAGCGGGAGC
    CATCTGGATT TTGGCCTGTC GGTGGTGCCG AATGAGTCAA CCGGCAGCAC GGTGAATGGC
    ATACCCACCC CAGACCCCAC AGGACAGGGG TACCTGGCTC AGCAGCTCCT CGGTGACAGC
    ACACACACCC AGGGGGCAGG CCGCTCCGGG CAGAACCTGC TGGGGCAGGC TAGTGCTACC
    TCACACATCT ATCAAGGGCC GGAGAGCAGC CTGCCCGGGC CTCCCAGCAT CAGCGTGCAG
    CCGCCAAACG TGGCAGCCGT CAGGGGCTAC CAGCCATGTG TTGGCTACGG GGGCAGCCGG
    CGCCAGGCTG GGCCGAGGGG TGGTCTCGCT CTGCAGCCAG GACAGTTTAG TGACCCGAGT
    CAGACCTGCA GGGTGAATGG CATCAAGATG GAGATGCCAG GACAGCCCCA TCAGCTCTGT
    TCGAATGTGC AGAGTTACTC TGGTCAGTTC TATGACCAAA CTGTTGGCTT CGGTCAGCAA
    GACATGAAAA CTGGTTCCTT CTCCATGTCA GAAGCCAACT GCCTGCTCCA GGGGGCCGGC
    GCCGAAAACT CTGAATTACT CTCCCCAGGT GCTAACCAGG TGACAAGCAC AGTTGACAGC
    CTCGACAGCC ATGACCTGGA AGGGGTGCAG ATTGATTTCG ACGCTATCAT AGACGACGGG
    GACCACGCCA GCCTGATGTC AGGAGCCCTG AGCCCAAGCA TTATTCAGAA CCTTTCTCAT
    AGCTCCTCCC GACTCACCAC GCCGCGAGCC GCCCTTCCGT TCCCGCCTCT GTCTTTGAGC
    ACCACCAACA TGGCAATCGG GGACATGAGC TCTCTGCTGA CCTCCCTCGC GGAAGAAAGC
    AAATTCCTTG CGGTTATGCA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005205716.1
    CDS229..4992
    Translation

    Target ORF information:

    RefSeq Version XM_005205659.3
    Organism Bos taurus(cattle)
    Definition Bos taurus GLI family zinc finger 3 (GLI3), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005205659.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGAGGCCC AGTCCCAGAG CTCGACGACC ACTGAGAAAA AGAAAGTGGA GAATTCCATA 
    GTGAAATGCC CCACTCGGCC AGATGTCAGC GAGAAAGCCG TGGCCTCCAG CACCACTTCC
    AATGAGGATG AAAGTCCTGG ACAGACCTAT CACAGAGAGA GAAGAAACGC AGTCACTATG
    CAGCCACAGA TTGCCCCGGG GCTCAGCAAG ATCAGCGAGG AGCCTTCAAC ATCCAGCGAC
    GAGAGGGCCT CACTGATCAA GAAGGAGATC CATGGGTCCT TGCCCCACCT GGCCGAGCCC
    TCTGTCCCCT ACCGCGGGGC TGTGTTTGCT ATGGACCCCA GGAATGGCTA CATGGAGCCT
    CACTATCACC CTCCTCATCT CTTTCCTGCA TTCCATCCTC CTGTACCAAT TGATGCCAGA
    CACCACGAGG GCCGTTACCA TTATGATCCA TCTCCGATCC CTCCGTTGCA TGTGCCTTCT
    GCCTTATCTA GTAGCCCCAC GTATTCTGAC CTGCCCTTCA TCAGGATCTC CCCACATCGG
    AACCCTGCTG CAGCTTCCGA ATCCCCCTTC AGCCCCCCGC ATCCCTACAT CAACCCCTAC
    ATGGACTACA TCCGGTCCCT GCACAGCAGC CCATCCCTCT CCATGATCTC GGCCGCTCGT
    GGGCTGAGCC CCACAGATGC CCCCCATGCC GGAGTCAGCC CAGCAGAATA CTATCATCAG
    ATGGCCCTGT TAGCCGGCCA GCGCAGCCCC TATGCAGACA TCATTCCCTC TGCTGCCACC
    GCTGGCGCAG GGGCCATCCA CATGGAGTAT CTTCACGCCA TGGATAGCAC CAGATTCCCC
    AGCCCTAGGC TGTCAGCCCG GCCGAGCCGA AAACGTACAC TGTCCATATC ACCACTGTCC
    GATCATAGCT TTGACCTTCA GACCATGATA AGGACATCTC CCAACTCCTT GGTCACGATT
    CTCAATAATT CCCGCAGCAG CTCTTCAGCA AGTGGCTCCT ATGGCCACTT ATCTGCAAGT
    GCAATCAGCC CTGCCCTGAG CTTCACCTAC CCTTCGGCGC CCGTGTCCCT CCACATGCAT
    CAGCAGATCC TAAGCCGACA ACAGAGCTTA GGCTCAGCCT TTGGACACAG CCCTCCGCTC
    ATCCACCCCG CCCCAACTTT CCCGACCCAG AGGCCTATTC CCGGAATCCC TACAGTTCTG
    AACCCCGTCC AGGTCAGCTC CGGCCCTTCC GAGTCCTCAC AGAACAAGCC CACGAGTGAG
    TCTGCAGTGA GCAGTACGGG GGACCTGGTG CACAACAAAC GCTCCAAGAT CAAACCAGAT
    GAAGACCTCC CCAGCCCAGG GCCACGGGGC CAGCAGGAAC AGCCAGAAGG AACAACCCTG
    GTGAAGGAGG AAGGGGACAA AGATGAAAGC AAACAGGAGC CCGAAGTCAT CTATGAGACA
    AACTGCCACT GGGAAGGCTG TTCTCGAGAG TTCGACACCC AGGAGCAGCT GGTGCATCAT
    ATAAATAATG ACCACATCCA TGGAGAGAAG AAGGAGTTTG TGTGCCGGTG GCTCGACTGC
    TCCAGAGAAC AGAAACCCTT CAAAGCCCAG TATATGCTGG TGGTGCACAT GCGCAGGCAC
    ACGGGGGAGA AGCCTCACAA ATGCACTTTT GAAGGTTGCA CAAAGGCCTA CTCGAGACTC
    GAAAACTTGA AAACACACTT GAGATCTCAC ACTGGAGAGA AACCATACGT CTGTGAGCAC
    GAAGGATGCA ACAAGGCTTT CTCTAATGCC TCGGATCGCG CTAAGCACCA GAACAGGACA
    CATTCCAACG AGAAACCATA TGTGTGCAAA ATCCCCGGCT GCACCAAGCG CTACACAGAC
    CCCAGCTCCC TCCGGAAACA CGTGAAGACC GTGCACGGCC CAGAGGCTCA TGTCACCAAG
    AAGCAGCGAG GGGACGTGCA TCCCCGGCCC CCGCCCCCTC GAGATTCCGG CAGCCACTCA
    CAGTCTAGGT CACCTGGCCG GCAGACTCAG GGAGCCCTCG GGGAGCAGAA GGACCTCAGC
    AACACTACCT CAAAGCGGGA AGAATGCCTC CAAGTGAAAA CCGTCAAGGC GGAGAAGCCC
    ATGACATCTC AGCCAAGCCC TGGTGGTCAG TCTTCATGCA GCAGCCAACA GTCCCCCATC
    AGCAACTATT CCAACAGTGG GCTCGAGCTT CCTCTGACCG ATGGAGGTGG TGTGGGCGAC
    CTCAGTGCCA TCGATGAAAC CCCAATCATG GACTCGACCA TTTCCACTGC AACCACAGCC
    CTCGCTTTGC AAGCCCGGAG GAACCCAGCA GGGACCAAAT GGATGGAGCA CGTCAAACTA
    GAAAGGCTCA AACAAGTGAA TGGAATGCTT CCACGACTGA ACCCCATTCT ACCCCCTAAA
    GCCCCTGCAG TCTCTCCTCT CATAGGAAAC GGCACGCAGC CCAGTACCAG CTGCAGTCTG
    GGCGGGCCCA TGCCCCTCCT CCCGAACAGA AGCGACCTGT CTGGGGTGGA CATCACCATG
    TTGAACATGC TCAACCGGAG GGACAGCAGC ACCAGCACCG TCAGCTCCGC CTACCTGAGC
    AGCCGCCGCT CCTCTGGCAT CTCGCCCTGC TTCTCCAGCC GCCGGTCCAG CGAGGCGTCC
    CAGGCCGAGG GGCGACCCCA GAACGTGAGC GTGGCCGACT CTTACGACCC CATCTCCACC
    GACGCCTCTC GCAGGTCCAG CGAGGCCAGC CAGGGCGACG GTCTGCCCAG CCTGCTCAGC
    CTCACTCCCG CCCAGCAGTA CCGCCTGAAA GCCAAGTACG CTGCCGCCAC GGGCGGCCCC
    CCGCCCACCC CGCTGCCCAA CATGGAGAGG ATGAGCCTCA AGACCCGGAT GGCCCTGTTG
    GGAGATGGCA GGGAGCCGGC GGGGACCCTG CCTCCCGTGC ATCCTCCTCG GAGATGCAGC
    GACGGCGGGG CCCACGGTTA CAGCCGGCGC CCACTGCTGC CCCAGGATGC GCTGGGCAAC
    GGCGTGAGAA GAGCCAGCGA CCCGGTGAGG ACGGTTTCGG AGAGCCTCTC TCTGCCCCGG
    GTACAGCGGT TCAACAGCCT TAACAGCTTC AACCCTCCGG TGTTGCCTCC ATCCCTGGAA
    AAGCGCAACC TCGTGCTTCA GAACTACACG CGGCCCGATG GGGGCCAGGC CCGCCACTTC
    CACTCCTCTC CCTGTCCCCC GAGCATCACT GAGAATGTCA CCCTGGAGTC CCTGACCATG
    GACGCCGACG TGAGCCTGAA CGATGAGGAC CTCCTGCCTG ATGACGTGGT GCAGTATTTA
    AATTCCCAGA ACCCAGCCGG GTATGAGCAG CACTTCCCAG GCACCTTCTC TGATGACAGC
    AAAGTGCCCC ATGGCTTAGG GAGTGGACCC GGGGACTTTG ACCCACTGGG GCTGCCCGAC
    AGCCACGCCA GCCACACCGG CAGCCAGCAG TACCGCAGCC TGGAGCAGCC CTGCCCCGAT
    GCCAGTAAAT CCGACCTGCC CATTCAGTGG AACGAAGTCA GCTCCGGCAG CGCGGACCTG
    TCCTCCTCCA AGCTGAAATG TGGCCAGCGG TCCATGGGGC AGCAGGCCCG GGCCTTCGGG
    TCGTACAGCC ACATGGGTGT TCACCCACAG AATCCATTGA GGAGTGGGGC TGGCCCAGCT
    GGGGGGTATC CGACCCTTGG GGAGCATGCC AGCTCCTTCG GGGGCCCGGA ACACTTAGTG
    AGCCACGGTG GCGGAGCTGG CACCAATGGG AATGCCTTCC ATGAACCACC CTATAAGGCC
    CAGCAGTATG GGAACTGCCT CAACAGGCAG CCTGGTGCCC CCACCTTACT CGATGGGGCC
    TGTGGCCCTG GGATCCAGGC GGCAAAGCTG AAAAGCATCA CCATGCAAGG GAGCGGGAGC
    CATCTGGATT TTGGCCTGTC GGTGGTGCCG AATGAGTCAA CCGGCAGCAC GGTGAATGGC
    ATACCCACCC CAGACCCCAC AGGACAGGGG TACCTGGCTC AGCAGCTCCT CGGTGACAGC
    ACACACACCC AGGGGGCAGG CCGCTCCGGG CAGAACCTGC TGGGGCAGGC TAGTGCTACC
    TCACACATCT ATCAAGGGCC GGAGAGCAGC CTGCCCGGGC CTCCCAGCAT CAGCGTGCAG
    CCGCCAAACG TGGCAGCCGT CAGGGGCTAC CAGCCATGTG TTGGCTACGG GGGCAGCCGG
    CGCCAGGCTG GGCCGAGGGG TGGTCTCGCT CTGCAGCCAG GACAGTTTAG TGACCCGAGT
    CAGACCTGCA GGGTGAATGG CATCAAGATG GAGATGCCAG GACAGCCCCA TCAGCTCTGT
    TCGAATGTGC AGAGTTACTC TGGTCAGTTC TATGACCAAA CTGTTGGCTT CGGTCAGCAA
    GACATGAAAA CTGGTTCCTT CTCCATGTCA GAAGCCAACT GCCTGCTCCA GGGGGCCGGC
    GCCGAAAACT CTGAATTACT CTCCCCAGGT GCTAACCAGG TGACAAGCAC AGTTGACAGC
    CTCGACAGCC ATGACCTGGA AGGGGTGCAG ATTGATTTCG ACGCTATCAT AGACGACGGG
    GACCACGCCA GCCTGATGTC AGGAGCCCTG AGCCCAAGCA TTATTCAGAA CCTTTCTCAT
    AGCTCCTCCC GACTCACCAC GCCGCGAGCC GCCCTTCCGT TCCCGCCTCT GTCTTTGAGC
    ACCACCAACA TGGCAATCGG GGACATGAGC TCTCTGCTGA CCTCCCTCGC GGAAGAAAGC
    AAATTCCTTG CGGTTATGCA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa18737
    Clone ID Related Accession (Same CDS sequence) XM_015463085.1 , XM_005205659.3 , XM_005205659.4
    Accession Version XM_005205659.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4764bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product transcriptional activator GLI3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005205659.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGAGGCCC AGTCCCAGAG CTCGACGACC ACTGAGAAAA AGAAAGTGGA GAATTCCATA 
    GTGAAATGCC CCACTCGGCC AGATGTCAGC GAGAAAGCCG TGGCCTCCAG CACCACTTCC
    AATGAGGATG AAAGTCCTGG ACAGACCTAT CACAGAGAGA GAAGAAACGC AGTCACTATG
    CAGCCACAGA TTGCCCCGGG GCTCAGCAAG ATCAGCGAGG AGCCTTCAAC ATCCAGCGAC
    GAGAGGGCCT CACTGATCAA GAAGGAGATC CATGGGTCCT TGCCCCACCT GGCCGAGCCC
    TCTGTCCCCT ACCGCGGGGC TGTGTTTGCT ATGGACCCCA GGAATGGCTA CATGGAGCCT
    CACTATCACC CTCCTCATCT CTTTCCTGCA TTCCATCCTC CTGTACCAAT TGATGCCAGA
    CACCACGAGG GCCGTTACCA TTATGATCCA TCTCCGATCC CTCCGTTGCA TGTGCCTTCT
    GCCTTATCTA GTAGCCCCAC GTATTCTGAC CTGCCCTTCA TCAGGATCTC CCCACATCGG
    AACCCTGCTG CAGCTTCCGA ATCCCCCTTC AGCCCCCCGC ATCCCTACAT CAACCCCTAC
    ATGGACTACA TCCGGTCCCT GCACAGCAGC CCATCCCTCT CCATGATCTC GGCCGCTCGT
    GGGCTGAGCC CCACAGATGC CCCCCATGCC GGAGTCAGCC CAGCAGAATA CTATCATCAG
    ATGGCCCTGT TAGCCGGCCA GCGCAGCCCC TATGCAGACA TCATTCCCTC TGCTGCCACC
    GCTGGCGCAG GGGCCATCCA CATGGAGTAT CTTCACGCCA TGGATAGCAC CAGATTCCCC
    AGCCCTAGGC TGTCAGCCCG GCCGAGCCGA AAACGTACAC TGTCCATATC ACCACTGTCC
    GATCATAGCT TTGACCTTCA GACCATGATA AGGACATCTC CCAACTCCTT GGTCACGATT
    CTCAATAATT CCCGCAGCAG CTCTTCAGCA AGTGGCTCCT ATGGCCACTT ATCTGCAAGT
    GCAATCAGCC CTGCCCTGAG CTTCACCTAC CCTTCGGCGC CCGTGTCCCT CCACATGCAT
    CAGCAGATCC TAAGCCGACA ACAGAGCTTA GGCTCAGCCT TTGGACACAG CCCTCCGCTC
    ATCCACCCCG CCCCAACTTT CCCGACCCAG AGGCCTATTC CCGGAATCCC TACAGTTCTG
    AACCCCGTCC AGGTCAGCTC CGGCCCTTCC GAGTCCTCAC AGAACAAGCC CACGAGTGAG
    TCTGCAGTGA GCAGTACGGG GGACCTGGTG CACAACAAAC GCTCCAAGAT CAAACCAGAT
    GAAGACCTCC CCAGCCCAGG GCCACGGGGC CAGCAGGAAC AGCCAGAAGG AACAACCCTG
    GTGAAGGAGG AAGGGGACAA AGATGAAAGC AAACAGGAGC CCGAAGTCAT CTATGAGACA
    AACTGCCACT GGGAAGGCTG TTCTCGAGAG TTCGACACCC AGGAGCAGCT GGTGCATCAT
    ATAAATAATG ACCACATCCA TGGAGAGAAG AAGGAGTTTG TGTGCCGGTG GCTCGACTGC
    TCCAGAGAAC AGAAACCCTT CAAAGCCCAG TATATGCTGG TGGTGCACAT GCGCAGGCAC
    ACGGGGGAGA AGCCTCACAA ATGCACTTTT GAAGGTTGCA CAAAGGCCTA CTCGAGACTC
    GAAAACTTGA AAACACACTT GAGATCTCAC ACTGGAGAGA AACCATACGT CTGTGAGCAC
    GAAGGATGCA ACAAGGCTTT CTCTAATGCC TCGGATCGCG CTAAGCACCA GAACAGGACA
    CATTCCAACG AGAAACCATA TGTGTGCAAA ATCCCCGGCT GCACCAAGCG CTACACAGAC
    CCCAGCTCCC TCCGGAAACA CGTGAAGACC GTGCACGGCC CAGAGGCTCA TGTCACCAAG
    AAGCAGCGAG GGGACGTGCA TCCCCGGCCC CCGCCCCCTC GAGATTCCGG CAGCCACTCA
    CAGTCTAGGT CACCTGGCCG GCAGACTCAG GGAGCCCTCG GGGAGCAGAA GGACCTCAGC
    AACACTACCT CAAAGCGGGA AGAATGCCTC CAAGTGAAAA CCGTCAAGGC GGAGAAGCCC
    ATGACATCTC AGCCAAGCCC TGGTGGTCAG TCTTCATGCA GCAGCCAACA GTCCCCCATC
    AGCAACTATT CCAACAGTGG GCTCGAGCTT CCTCTGACCG ATGGAGGTGG TGTGGGCGAC
    CTCAGTGCCA TCGATGAAAC CCCAATCATG GACTCGACCA TTTCCACTGC AACCACAGCC
    CTCGCTTTGC AAGCCCGGAG GAACCCAGCA GGGACCAAAT GGATGGAGCA CGTCAAACTA
    GAAAGGCTCA AACAAGTGAA TGGAATGCTT CCACGACTGA ACCCCATTCT ACCCCCTAAA
    GCCCCTGCAG TCTCTCCTCT CATAGGAAAC GGCACGCAGC CCAGTACCAG CTGCAGTCTG
    GGCGGGCCCA TGCCCCTCCT CCCGAACAGA AGCGACCTGT CTGGGGTGGA CATCACCATG
    TTGAACATGC TCAACCGGAG GGACAGCAGC ACCAGCACCG TCAGCTCCGC CTACCTGAGC
    AGCCGCCGCT CCTCTGGCAT CTCGCCCTGC TTCTCCAGCC GCCGGTCCAG CGAGGCGTCC
    CAGGCCGAGG GGCGACCCCA GAACGTGAGC GTGGCCGACT CTTACGACCC CATCTCCACC
    GACGCCTCTC GCAGGTCCAG CGAGGCCAGC CAGGGCGACG GTCTGCCCAG CCTGCTCAGC
    CTCACTCCCG CCCAGCAGTA CCGCCTGAAA GCCAAGTACG CTGCCGCCAC GGGCGGCCCC
    CCGCCCACCC CGCTGCCCAA CATGGAGAGG ATGAGCCTCA AGACCCGGAT GGCCCTGTTG
    GGAGATGGCA GGGAGCCGGC GGGGACCCTG CCTCCCGTGC ATCCTCCTCG GAGATGCAGC
    GACGGCGGGG CCCACGGTTA CAGCCGGCGC CCACTGCTGC CCCAGGATGC GCTGGGCAAC
    GGCGTGAGAA GAGCCAGCGA CCCGGTGAGG ACGGTTTCGG AGAGCCTCTC TCTGCCCCGG
    GTACAGCGGT TCAACAGCCT TAACAGCTTC AACCCTCCGG TGTTGCCTCC ATCCCTGGAA
    AAGCGCAACC TCGTGCTTCA GAACTACACG CGGCCCGATG GGGGCCAGGC CCGCCACTTC
    CACTCCTCTC CCTGTCCCCC GAGCATCACT GAGAATGTCA CCCTGGAGTC CCTGACCATG
    GACGCCGACG TGAGCCTGAA CGATGAGGAC CTCCTGCCTG ATGACGTGGT GCAGTATTTA
    AATTCCCAGA ACCCAGCCGG GTATGAGCAG CACTTCCCAG GCACCTTCTC TGATGACAGC
    AAAGTGCCCC ATGGCTTAGG GAGTGGACCC GGGGACTTTG ACCCACTGGG GCTGCCCGAC
    AGCCACGCCA GCCACACCGG CAGCCAGCAG TACCGCAGCC TGGAGCAGCC CTGCCCCGAT
    GCCAGTAAAT CCGACCTGCC CATTCAGTGG AACGAAGTCA GCTCCGGCAG CGCGGACCTG
    TCCTCCTCCA AGCTGAAATG TGGCCAGCGG TCCATGGGGC AGCAGGCCCG GGCCTTCGGG
    TCGTACAGCC ACATGGGTGT TCACCCACAG AATCCATTGA GGAGTGGGGC TGGCCCAGCT
    GGGGGGTATC CGACCCTTGG GGAGCATGCC AGCTCCTTCG GGGGCCCGGA ACACTTAGTG
    AGCCACGGTG GCGGAGCTGG CACCAATGGG AATGCCTTCC ATGAACCACC CTATAAGGCC
    CAGCAGTATG GGAACTGCCT CAACAGGCAG CCTGGTGCCC CCACCTTACT CGATGGGGCC
    TGTGGCCCTG GGATCCAGGC GGCAAAGCTG AAAAGCATCA CCATGCAAGG GAGCGGGAGC
    CATCTGGATT TTGGCCTGTC GGTGGTGCCG AATGAGTCAA CCGGCAGCAC GGTGAATGGC
    ATACCCACCC CAGACCCCAC AGGACAGGGG TACCTGGCTC AGCAGCTCCT CGGTGACAGC
    ACACACACCC AGGGGGCAGG CCGCTCCGGG CAGAACCTGC TGGGGCAGGC TAGTGCTACC
    TCACACATCT ATCAAGGGCC GGAGAGCAGC CTGCCCGGGC CTCCCAGCAT CAGCGTGCAG
    CCGCCAAACG TGGCAGCCGT CAGGGGCTAC CAGCCATGTG TTGGCTACGG GGGCAGCCGG
    CGCCAGGCTG GGCCGAGGGG TGGTCTCGCT CTGCAGCCAG GACAGTTTAG TGACCCGAGT
    CAGACCTGCA GGGTGAATGG CATCAAGATG GAGATGCCAG GACAGCCCCA TCAGCTCTGT
    TCGAATGTGC AGAGTTACTC TGGTCAGTTC TATGACCAAA CTGTTGGCTT CGGTCAGCAA
    GACATGAAAA CTGGTTCCTT CTCCATGTCA GAAGCCAACT GCCTGCTCCA GGGGGCCGGC
    GCCGAAAACT CTGAATTACT CTCCCCAGGT GCTAACCAGG TGACAAGCAC AGTTGACAGC
    CTCGACAGCC ATGACCTGGA AGGGGTGCAG ATTGATTTCG ACGCTATCAT AGACGACGGG
    GACCACGCCA GCCTGATGTC AGGAGCCCTG AGCCCAAGCA TTATTCAGAA CCTTTCTCAT
    AGCTCCTCCC GACTCACCAC GCCGCGAGCC GCCCTTCCGT TCCCGCCTCT GTCTTTGAGC
    ACCACCAACA TGGCAATCGG GGACATGAGC TCTCTGCTGA CCTCCCTCGC GGAAGAAAGC
    AAATTCCTTG CGGTTATGCA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005205716.1
    CDS123..4886
    Translation

    Target ORF information:

    RefSeq Version XM_005205659.4
    Organism Bos taurus(cattle)
    Definition Bos taurus GLI family zinc finger 3 (GLI3), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005205659.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGAGGCCC AGTCCCAGAG CTCGACGACC ACTGAGAAAA AGAAAGTGGA GAATTCCATA 
    GTGAAATGCC CCACTCGGCC AGATGTCAGC GAGAAAGCCG TGGCCTCCAG CACCACTTCC
    AATGAGGATG AAAGTCCTGG ACAGACCTAT CACAGAGAGA GAAGAAACGC AGTCACTATG
    CAGCCACAGA TTGCCCCGGG GCTCAGCAAG ATCAGCGAGG AGCCTTCAAC ATCCAGCGAC
    GAGAGGGCCT CACTGATCAA GAAGGAGATC CATGGGTCCT TGCCCCACCT GGCCGAGCCC
    TCTGTCCCCT ACCGCGGGGC TGTGTTTGCT ATGGACCCCA GGAATGGCTA CATGGAGCCT
    CACTATCACC CTCCTCATCT CTTTCCTGCA TTCCATCCTC CTGTACCAAT TGATGCCAGA
    CACCACGAGG GCCGTTACCA TTATGATCCA TCTCCGATCC CTCCGTTGCA TGTGCCTTCT
    GCCTTATCTA GTAGCCCCAC GTATTCTGAC CTGCCCTTCA TCAGGATCTC CCCACATCGG
    AACCCTGCTG CAGCTTCCGA ATCCCCCTTC AGCCCCCCGC ATCCCTACAT CAACCCCTAC
    ATGGACTACA TCCGGTCCCT GCACAGCAGC CCATCCCTCT CCATGATCTC GGCCGCTCGT
    GGGCTGAGCC CCACAGATGC CCCCCATGCC GGAGTCAGCC CAGCAGAATA CTATCATCAG
    ATGGCCCTGT TAGCCGGCCA GCGCAGCCCC TATGCAGACA TCATTCCCTC TGCTGCCACC
    GCTGGCGCAG GGGCCATCCA CATGGAGTAT CTTCACGCCA TGGATAGCAC CAGATTCCCC
    AGCCCTAGGC TGTCAGCCCG GCCGAGCCGA AAACGTACAC TGTCCATATC ACCACTGTCC
    GATCATAGCT TTGACCTTCA GACCATGATA AGGACATCTC CCAACTCCTT GGTCACGATT
    CTCAATAATT CCCGCAGCAG CTCTTCAGCA AGTGGCTCCT ATGGCCACTT ATCTGCAAGT
    GCAATCAGCC CTGCCCTGAG CTTCACCTAC CCTTCGGCGC CCGTGTCCCT CCACATGCAT
    CAGCAGATCC TAAGCCGACA ACAGAGCTTA GGCTCAGCCT TTGGACACAG CCCTCCGCTC
    ATCCACCCCG CCCCAACTTT CCCGACCCAG AGGCCTATTC CCGGAATCCC TACAGTTCTG
    AACCCCGTCC AGGTCAGCTC CGGCCCTTCC GAGTCCTCAC AGAACAAGCC CACGAGTGAG
    TCTGCAGTGA GCAGTACGGG GGACCTGGTG CACAACAAAC GCTCCAAGAT CAAACCAGAT
    GAAGACCTCC CCAGCCCAGG GCCACGGGGC CAGCAGGAAC AGCCAGAAGG AACAACCCTG
    GTGAAGGAGG AAGGGGACAA AGATGAAAGC AAACAGGAGC CCGAAGTCAT CTATGAGACA
    AACTGCCACT GGGAAGGCTG TTCTCGAGAG TTCGACACCC AGGAGCAGCT GGTGCATCAT
    ATAAATAATG ACCACATCCA TGGAGAGAAG AAGGAGTTTG TGTGCCGGTG GCTCGACTGC
    TCCAGAGAAC AGAAACCCTT CAAAGCCCAG TATATGCTGG TGGTGCACAT GCGCAGGCAC
    ACGGGGGAGA AGCCTCACAA ATGCACTTTT GAAGGTTGCA CAAAGGCCTA CTCGAGACTC
    GAAAACTTGA AAACACACTT GAGATCTCAC ACTGGAGAGA AACCATACGT CTGTGAGCAC
    GAAGGATGCA ACAAGGCTTT CTCTAATGCC TCGGATCGCG CTAAGCACCA GAACAGGACA
    CATTCCAACG AGAAACCATA TGTGTGCAAA ATCCCCGGCT GCACCAAGCG CTACACAGAC
    CCCAGCTCCC TCCGGAAACA CGTGAAGACC GTGCACGGCC CAGAGGCTCA TGTCACCAAG
    AAGCAGCGAG GGGACGTGCA TCCCCGGCCC CCGCCCCCTC GAGATTCCGG CAGCCACTCA
    CAGTCTAGGT CACCTGGCCG GCAGACTCAG GGAGCCCTCG GGGAGCAGAA GGACCTCAGC
    AACACTACCT CAAAGCGGGA AGAATGCCTC CAAGTGAAAA CCGTCAAGGC GGAGAAGCCC
    ATGACATCTC AGCCAAGCCC TGGTGGTCAG TCTTCATGCA GCAGCCAACA GTCCCCCATC
    AGCAACTATT CCAACAGTGG GCTCGAGCTT CCTCTGACCG ATGGAGGTGG TGTGGGCGAC
    CTCAGTGCCA TCGATGAAAC CCCAATCATG GACTCGACCA TTTCCACTGC AACCACAGCC
    CTCGCTTTGC AAGCCCGGAG GAACCCAGCA GGGACCAAAT GGATGGAGCA CGTCAAACTA
    GAAAGGCTCA AACAAGTGAA TGGAATGCTT CCACGACTGA ACCCCATTCT ACCCCCTAAA
    GCCCCTGCAG TCTCTCCTCT CATAGGAAAC GGCACGCAGC CCAGTACCAG CTGCAGTCTG
    GGCGGGCCCA TGCCCCTCCT CCCGAACAGA AGCGACCTGT CTGGGGTGGA CATCACCATG
    TTGAACATGC TCAACCGGAG GGACAGCAGC ACCAGCACCG TCAGCTCCGC CTACCTGAGC
    AGCCGCCGCT CCTCTGGCAT CTCGCCCTGC TTCTCCAGCC GCCGGTCCAG CGAGGCGTCC
    CAGGCCGAGG GGCGACCCCA GAACGTGAGC GTGGCCGACT CTTACGACCC CATCTCCACC
    GACGCCTCTC GCAGGTCCAG CGAGGCCAGC CAGGGCGACG GTCTGCCCAG CCTGCTCAGC
    CTCACTCCCG CCCAGCAGTA CCGCCTGAAA GCCAAGTACG CTGCCGCCAC GGGCGGCCCC
    CCGCCCACCC CGCTGCCCAA CATGGAGAGG ATGAGCCTCA AGACCCGGAT GGCCCTGTTG
    GGAGATGGCA GGGAGCCGGC GGGGACCCTG CCTCCCGTGC ATCCTCCTCG GAGATGCAGC
    GACGGCGGGG CCCACGGTTA CAGCCGGCGC CCACTGCTGC CCCAGGATGC GCTGGGCAAC
    GGCGTGAGAA GAGCCAGCGA CCCGGTGAGG ACGGTTTCGG AGAGCCTCTC TCTGCCCCGG
    GTACAGCGGT TCAACAGCCT TAACAGCTTC AACCCTCCGG TGTTGCCTCC ATCCCTGGAA
    AAGCGCAACC TCGTGCTTCA GAACTACACG CGGCCCGATG GGGGCCAGGC CCGCCACTTC
    CACTCCTCTC CCTGTCCCCC GAGCATCACT GAGAATGTCA CCCTGGAGTC CCTGACCATG
    GACGCCGACG TGAGCCTGAA CGATGAGGAC CTCCTGCCTG ATGACGTGGT GCAGTATTTA
    AATTCCCAGA ACCCAGCCGG GTATGAGCAG CACTTCCCAG GCACCTTCTC TGATGACAGC
    AAAGTGCCCC ATGGCTTAGG GAGTGGACCC GGGGACTTTG ACCCACTGGG GCTGCCCGAC
    AGCCACGCCA GCCACACCGG CAGCCAGCAG TACCGCAGCC TGGAGCAGCC CTGCCCCGAT
    GCCAGTAAAT CCGACCTGCC CATTCAGTGG AACGAAGTCA GCTCCGGCAG CGCGGACCTG
    TCCTCCTCCA AGCTGAAATG TGGCCAGCGG TCCATGGGGC AGCAGGCCCG GGCCTTCGGG
    TCGTACAGCC ACATGGGTGT TCACCCACAG AATCCATTGA GGAGTGGGGC TGGCCCAGCT
    GGGGGGTATC CGACCCTTGG GGAGCATGCC AGCTCCTTCG GGGGCCCGGA ACACTTAGTG
    AGCCACGGTG GCGGAGCTGG CACCAATGGG AATGCCTTCC ATGAACCACC CTATAAGGCC
    CAGCAGTATG GGAACTGCCT CAACAGGCAG CCTGGTGCCC CCACCTTACT CGATGGGGCC
    TGTGGCCCTG GGATCCAGGC GGCAAAGCTG AAAAGCATCA CCATGCAAGG GAGCGGGAGC
    CATCTGGATT TTGGCCTGTC GGTGGTGCCG AATGAGTCAA CCGGCAGCAC GGTGAATGGC
    ATACCCACCC CAGACCCCAC AGGACAGGGG TACCTGGCTC AGCAGCTCCT CGGTGACAGC
    ACACACACCC AGGGGGCAGG CCGCTCCGGG CAGAACCTGC TGGGGCAGGC TAGTGCTACC
    TCACACATCT ATCAAGGGCC GGAGAGCAGC CTGCCCGGGC CTCCCAGCAT CAGCGTGCAG
    CCGCCAAACG TGGCAGCCGT CAGGGGCTAC CAGCCATGTG TTGGCTACGG GGGCAGCCGG
    CGCCAGGCTG GGCCGAGGGG TGGTCTCGCT CTGCAGCCAG GACAGTTTAG TGACCCGAGT
    CAGACCTGCA GGGTGAATGG CATCAAGATG GAGATGCCAG GACAGCCCCA TCAGCTCTGT
    TCGAATGTGC AGAGTTACTC TGGTCAGTTC TATGACCAAA CTGTTGGCTT CGGTCAGCAA
    GACATGAAAA CTGGTTCCTT CTCCATGTCA GAAGCCAACT GCCTGCTCCA GGGGGCCGGC
    GCCGAAAACT CTGAATTACT CTCCCCAGGT GCTAACCAGG TGACAAGCAC AGTTGACAGC
    CTCGACAGCC ATGACCTGGA AGGGGTGCAG ATTGATTTCG ACGCTATCAT AGACGACGGG
    GACCACGCCA GCCTGATGTC AGGAGCCCTG AGCCCAAGCA TTATTCAGAA CCTTTCTCAT
    AGCTCCTCCC GACTCACCAC GCCGCGAGCC GCCCTTCCGT TCCCGCCTCT GTCTTTGAGC
    ACCACCAACA TGGCAATCGG GGACATGAGC TCTCTGCTGA CCTCCCTCGC GGAAGAAAGC
    AAATTCCTTG CGGTTATGCA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.