ARHGEF28 cDNA ORF clone, Bos taurus(cattle)

The following ARHGEF28 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ARHGEF28 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa228079 XM_024981393.1
Latest version!
Bos taurus Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa228080 XM_024981394.1
Latest version!
Bos taurus Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa228081 XM_024981395.1
Latest version!
Bos taurus Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa228082 XM_024981396.1
Latest version!
Bos taurus Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa228083 XM_024981398.1
Latest version!
Bos taurus Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa228083 XM_024981397.1
Latest version!
Bos taurus Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa228083 XM_024981399.1
Latest version!
Bos taurus Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa228084 XM_024981400.1
Latest version!
Bos taurus Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa228085 XM_024981401.1
Latest version!
Bos taurus Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa228086 XM_024981402.1
Latest version!
Bos taurus Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa228087 XM_024981403.1
Latest version!
Bos taurus Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa39032 NM_001102307.1
Latest version!
Bos taurus Rho guanine nucleotide exchange factor 28 (ARHGEF28), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa228079
    Clone ID Related Accession (Same CDS sequence) XM_024981393.1
    Accession Version XM_024981393.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5406bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product rho guanine nucleotide exchange factor 28 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGCAGCCCA CTCTTCACTT TCTTCCCATC TTTAGGTGTC CGTTCAGCTG TGAGCTCTGT 
    GAGGAGTGGA TCGTTTCCTG GTCATCTTTG TGCAATGCTG AGCCCATTGA AGATGCGGAA
    ACCATGGAGC TGAACTGCAG CGAAGTACCT CTGTATGGGC AGATGACGGT CTGCGCGAAG
    TTTGGCAAAA ATGTTTATCT TCCTAAAGAT GCTGAGTTTT ACTTTATTTA TAATGGATCT
    CATCAGAGAC ACATCGTGAT TGCAGAGCGC ACAGAAGATA ATGTTCTCCA GTCCAGCATT
    CCAGGCCACG GGCTTCAGGA GACGGTCACC GTGTCTGTGT GCCTGTGCTC GGAAGGTTAC
    TCCCCGGTGA CCATGGGCTC TGACTCCGTG ACCTACGTGG ACAACATGGC CTGCAGGCTG
    GCCCGTCTGC TGGTCACCCA GGCCGATCGC CTCACCGCCA GCAGCCACCA GACCTTGCTG
    ACCCCGTTTG CCCTGACGGC GGGGGCATTG CCTGCTCTGG ACGAGGAGCT CGTGCTGGCC
    CTGACTCATC TGGAGTTGCC TCTGGGGTGG ACCGTCTTGG GGAATTCTTC ACTGGAAGTT
    TCTCTGCATA GGGAGTCTCT TCTGCACCTG GCTATGAGAT GGGGCCTTGC TAAGCTTTCC
    CAGCTCCTTC TGTGTCTCCC TGGGGGAGCC CAGGCCCTGA CTTTACCCAA CGAGGAGGGT
    GCCACGCCAT TAGACTTGGC TTTACATGGT GGACATTCCA AGCTGGTTGA AGACATCACA
    AACTTCCAGG GCAGGCGTTC CCCAGGTTTC TCCTGCGTGC AGCTCAGCGA AGATGCCTCT
    CTGCGGTATA TTCACTCATC AGAAACTCTG ACCCTGACGC TTAACCACAC AGCCGAGCAT
    TTGCTGGAGG CGGATATTAA ACTCTTCAGA AAATACTTTT GGGATAGAGC CTTTCTCATC
    AAGGCTCTTG AAGAGCAAGA AGGCACGTCA GAGGAGAAAC CAGATATGCC CTCCAGCGCT
    GCAGAAACTG AAGAAGGTAT GCATGCTCCT TCCCCACCTG GGGAACTCAG AAAGTGCTCA
    GCATTCTCTA GTCAATTGCG AGTTGAAGTG TGTGGAGAAG GAGAGCAGGA GCCGATGGAC
    TCGGAGGCGA GAGTAGGTTG CTCTGGGGCC GCAGGGCCCA GAGTTCCTTC AGCAGCACTG
    ACCAAAGAGA TTAAGAATTC GGGGTGCAGC AGATCACCAC CCGGGAAGGA GGGTGTAAAG
    CGTGTGAAAA GCCTGGTGGT TCCACTGAAT GAACATGAGG ACCAGGAGAG GCTAGATTTG
    GATCGTTCCT TTGATGCCCT CAAAAAATCC AAGCACCCCT CAACATTGCT TGCTGCTGGC
    CAGCTCTCTG ACATGCTGAA TGGTGGTGAT GACGTCTACG CTAACTGCAT GGTGATAGAC
    CAGGTTGGTG ATTTGGACAT TAACTATATT AATATAGAGG AAATCCCTGC AAATACCTGT
    CTTGAATCCA TGGATTCTTC TCTGGGGCCT GAGAGCAGCA AACCCAGCCT TCCTACTGAA
    GCCAGTGCTG GCACATACCC ACCAAATAAG AGGCCAGAAG TGACGTCACA CACGGAAGCC
    CAATCATCTT TCATATCATC TAGTTCGTAT GCTTCCAATT TGAATCTTTC TTTTGGTCTT
    CGTGGATTTG AAAAGGAGCA AAGTCGTCTA AAGAAAAGAA GTTCCAGCCT TGATGCCTTG
    GATGCTGACA GTGAAGGAGA GGGCCATTCG GAGCAGTCGC ACAATTGCTA CACTCCCGTG
    TGTCAGAGTT CCTCGAGAAC TGGCATTGCT AGCGGGGATG AATTGGACTC TTTTGAGACC
    AACACTGAAC CAGATTTTAA TATCTCCAGG ACGGAATCAT TTTCTTTATC AAGTAGCCTG
    CAGCTGAAGG AATCATTGTT TTCTGGAATC CGTTCACGTT CGTATTCCTG CTCATCACCC
    AAAATTTCTT TAGGAAAAAC TCGATTGATG CGTGATTTTA CAGTGTGTAA TTCAATTGAA
    GAGCAAAGAG CTTACAGCTT GCCGGAGCCA CCGAGAGAAC AAAGAATTCA GGAAGAAGAA
    TGGGATAAAT ATATCATACC TGCCAAATCA GAATCTGAAA AATACAAAGT AAGCCGAACT
    TTTAGCTTCC TCATGAATAG GATGACCAGC CCTCGGAATA AATCAAAGAC AAAAGGCAAG
    GATGCCAAAG ACAAAGAGAA ACTGACTCGA CATCAGTTTG TCCCTGGAAC TTTCTCTGGG
    GTTCTGCAGT GTTTGGTTTG CGATAAAACG CTCCTGGGGA AAGAGTCATT CCAGTGCTCC
    AACTGTAATG GAAACGTACA TAAAGTCTGT AAAGATGCTG CCCCTCCGTG TACCAAGAAA
    TTCCAAGACA AATATAATAA GAACAAACCA CAGACCATCC TTGGAAATTC TTCGTTTCGA
    GACGTCCCAT TGCCTGGGCT CTCTCTGCAC CCTTCCTCCT CCATGCCTAT CGGGTTGCCA
    ACTGGGAGGA AGGAGATGGC AGGGCAAGTG CATCCCTTGT CCAGAAGTGT CCCAGGCACC
    ACCTTGGAAA GCTTCAGGAG GCCGGGGGCA TCCTTGGAGT CCGAGAATGA TGGCAGCACC
    TGGAGAAGCA GGTCTCACTC TGACGAGCTG TTCCAGCCCA TGGGCTCGTC CCCCTCCACG
    GACTCCTTCT TAACGGAAGA TGTCGTGGAT TCTTCTCTGT GGAGTGACCT CAGCGGCGAT
    GCCCAGGAGT TTGAAGCGGA ATCTTGGAGT CTGGTGGTGG ACCCTTCATT TTGTAGTCGG
    CAGGAGAAGG ATGTCATCCA AAGACAGGAT GTCATTTTTG AGCTAATGCA GACAGAACTG
    CATCACATCC AGACCCTGTT CATCATGTCT GAGATCTTCA GGAAAGGGAT GAAAGAGGAG
    CTGCAGCTGG ACCACAGCAC TGTGGACAAA ATCTTCCCCT GTCTGGACGA GTTACTTGAA
    ATCCACAAGC ATTTCTTCTG TAGTATGAAG GAGCGAAGGC AGGAATCCTG TGTTGGCAAT
    GACAGGAATT TCATCATCGG CCGAATTGGA GATATTCTAG TACAACAGTT TTCAGAAGAA
    AATGCAAATA AAATGAAGAA AATATATGGG GAATTCTGTA GCCATCACAA AGAGGCTGTT
    AGCCTCTTTA AAGAACTCCA GCAGAATAAA AAGTTTCAGA ATTTTATTAA GCTCCGAAAT
    AGTAACCTTC TGGCTCGACG CCGAGGAATT CCAGAATGCA TTCTGCTGGT CACTCAGCGC
    ATCACAAAAT ACCCTGTCTT GGTGGAAAGG ATATTGCAGT ACACAAAGGA GAGCACTGAA
    GAACAGAGAG ACTTATGCAA AGCTCTTTGC TTAATTAAAG ACATGATTGC AGCTGTGGAT
    TTAAAAGTCA GCGAATATGA GAAAAAACAA AAATGGCTTG AGATCCTAAA TAAGATCGAA
    AACAAAACAT ACACCAAGCT CAAAAATGGC CATGTGTTTA GGAAACAGGC ACTGATGAGT
    AAAGAGAGGA CCCTGTTATA TGATGGCCTT GTCTATTGGA AAACTGCTAC AGGTCGTTTC
    AAAGATATCC TAGCTCTACT TCTAACTGAT GTGCTGCTCT TTTTACAAGA AAAAGACCAG
    AAATACATCT TTGCAGCTGT TGATCAGAAG CCATCAGTTA TTTCCCTTCA GAAGCTCATT
    GCTAGGGAAG TTGCGAATGA GGAGAGAGGA ATGTTTTTGA TCAGCGCTTC ATCTGCTGGT
    CCCGAGATGT ATGAAATCCA TACCAACTCC AAGGAGGAAA GAAATACCTG GATGAGACGG
    ATCCAACAGG CAGTAGAAAG TTGCCCAGAA GAAGAAGGGG GAAGGACAAG TGAGTCTGAT
    GAAGAGAGGA GGAAAGCTGA AGCCAGAGTG GCGAAGATTC AGCAATGCCA AGAAATACTC
    AGTAACCAAG ATCAACAAAT TTGCACATAT TTGGAAGAGA AACTGCATAT CTACGCGAAA
    CTTGGAGAAC TAAGTGGATT TCAGGATGTC CATCTAGAGC CCCACCTCCT CATTCAACCA
    GACCCCGGAG AGCCTCCCCA GGCAGCCTCA TTACTGGCCG CAGCACTGAA AGAGGCTGAG
    AGTCTGCAGG TGGCAGTGAA GGCTTCCCAG ATGAGCGATG CATGCCAGTC CCCCGAGGAA
    GGTTGTGGAG AACCTGCTTT GCCGGACGTG CTCCATTCTC AGGACGCACC AGGATCGTCT
    ACTGCTTCAC TAGTCACAGA AGGGACAGGA GGAAGAGGCT GTTGGGATGT GGATGCCGGG
    ACCCAGGGTG TGGGAACCGA CCTGGCGGTT TCTGATGCGG GGGAGAAGGT GGAATATAGA
    AGTTTCCCAG GTTCTTTGCA GTCAGAGATT ATACAAGCGA TACAGAATTT AACTCGTCTC
    TTATATAGCC TTCAGGCCGC CTTGACCATT CAGGATAGCC ACATTGAGAT CCACAGGCTG
    GTTCTCCAAC AGCGGGAGAG CCTGGCCCCG GGCCCCGCGT TCCGAGGCAG CCCCTTTCAG
    GACCAGGAGA AGTCCCGCAG CCTGGAGAAG CAGCGGGAGG AGCTGGCCGA TACCCACAAG
    CTGCAGCTCC AGTTCCAGCA AGACCAGCAC CGCTGGCTCC GCAGGTGCGA CCAGCAGCAG
    CGCGAGCAGG AGGCCCGGGC CAGCCGGCTG CAGGAGCGCG AGAGGGAGTG CCGGGCGCAG
    GAGGAGCTGC TGCTCAGGAG CCGGGGCGAG CTGGACCAGC AGCTCCAAGA CTACCAGCAG
    AGCCTGGAGC GCCTGCGCGA GGGCCAGCGC CTGGTGGAGA GGGAGCGGGA GAGGATGCGG
    GCCCAGCAGA GCCTGCTGCG CCACTGGAAG CACGGGCGGC AGAGCAGCCT TCCCGCCGCG
    TTTTCTCCAG GAGCCCTGGA GGTAATGGAA CTTAATCGAT CTGAGACTTT ACGTCATGAA
    AATTCATTCT TCATCAATGA AGCTTTAGTG CAAATGTCAC TCAACCCTTT GAACAAACCG
    CCGCCACCAG GTATCCATCA GGATGCGGCT TACCCCCCAG GCATACCTCA TTCTGATTTG
    GTAAGGACTA CTGAAAATCA AGTAGACCTC AAGACGGACG TTTCTCAACC CTCAGATATC
    AACCATGAAC TGTGGATAGC GGCTGGGGCC TGTCATCAGA TACCCCCTCT CCACCAAAGC
    AGCAAGGATT CTTGTAAAAA TGATGTGAAC ATCTCTCAGA CTGCATACCC ACCCCTCCAT
    GACTCAAATC CACACAGCCC TCGGCTGCAG ACGTTTATAG CAGAAGAAAA GCTAAATCTA
    CAGAGAGTGA CCAGACAAGA CGGGGAAACT GGAAATGGAG CAGAAGAAAA TATTGTTTAC
    CTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024837161.1
    CDS28..5433
    Translation

    Target ORF information:

    RefSeq Version XM_024981393.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981393.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGCAGCCCA CTCTTCACTT TCTTCCCATC TTTAGGTGTC CGTTCAGCTG TGAGCTCTGT 
    GAGGAGTGGA TCGTTTCCTG GTCATCTTTG TGCAATGCTG AGCCCATTGA AGATGCGGAA
    ACCATGGAGC TGAACTGCAG CGAAGTACCT CTGTATGGGC AGATGACGGT CTGCGCGAAG
    TTTGGCAAAA ATGTTTATCT TCCTAAAGAT GCTGAGTTTT ACTTTATTTA TAATGGATCT
    CATCAGAGAC ACATCGTGAT TGCAGAGCGC ACAGAAGATA ATGTTCTCCA GTCCAGCATT
    CCAGGCCACG GGCTTCAGGA GACGGTCACC GTGTCTGTGT GCCTGTGCTC GGAAGGTTAC
    TCCCCGGTGA CCATGGGCTC TGACTCCGTG ACCTACGTGG ACAACATGGC CTGCAGGCTG
    GCCCGTCTGC TGGTCACCCA GGCCGATCGC CTCACCGCCA GCAGCCACCA GACCTTGCTG
    ACCCCGTTTG CCCTGACGGC GGGGGCATTG CCTGCTCTGG ACGAGGAGCT CGTGCTGGCC
    CTGACTCATC TGGAGTTGCC TCTGGGGTGG ACCGTCTTGG GGAATTCTTC ACTGGAAGTT
    TCTCTGCATA GGGAGTCTCT TCTGCACCTG GCTATGAGAT GGGGCCTTGC TAAGCTTTCC
    CAGCTCCTTC TGTGTCTCCC TGGGGGAGCC CAGGCCCTGA CTTTACCCAA CGAGGAGGGT
    GCCACGCCAT TAGACTTGGC TTTACATGGT GGACATTCCA AGCTGGTTGA AGACATCACA
    AACTTCCAGG GCAGGCGTTC CCCAGGTTTC TCCTGCGTGC AGCTCAGCGA AGATGCCTCT
    CTGCGGTATA TTCACTCATC AGAAACTCTG ACCCTGACGC TTAACCACAC AGCCGAGCAT
    TTGCTGGAGG CGGATATTAA ACTCTTCAGA AAATACTTTT GGGATAGAGC CTTTCTCATC
    AAGGCTCTTG AAGAGCAAGA AGGCACGTCA GAGGAGAAAC CAGATATGCC CTCCAGCGCT
    GCAGAAACTG AAGAAGGTAT GCATGCTCCT TCCCCACCTG GGGAACTCAG AAAGTGCTCA
    GCATTCTCTA GTCAATTGCG AGTTGAAGTG TGTGGAGAAG GAGAGCAGGA GCCGATGGAC
    TCGGAGGCGA GAGTAGGTTG CTCTGGGGCC GCAGGGCCCA GAGTTCCTTC AGCAGCACTG
    ACCAAAGAGA TTAAGAATTC GGGGTGCAGC AGATCACCAC CCGGGAAGGA GGGTGTAAAG
    CGTGTGAAAA GCCTGGTGGT TCCACTGAAT GAACATGAGG ACCAGGAGAG GCTAGATTTG
    GATCGTTCCT TTGATGCCCT CAAAAAATCC AAGCACCCCT CAACATTGCT TGCTGCTGGC
    CAGCTCTCTG ACATGCTGAA TGGTGGTGAT GACGTCTACG CTAACTGCAT GGTGATAGAC
    CAGGTTGGTG ATTTGGACAT TAACTATATT AATATAGAGG AAATCCCTGC AAATACCTGT
    CTTGAATCCA TGGATTCTTC TCTGGGGCCT GAGAGCAGCA AACCCAGCCT TCCTACTGAA
    GCCAGTGCTG GCACATACCC ACCAAATAAG AGGCCAGAAG TGACGTCACA CACGGAAGCC
    CAATCATCTT TCATATCATC TAGTTCGTAT GCTTCCAATT TGAATCTTTC TTTTGGTCTT
    CGTGGATTTG AAAAGGAGCA AAGTCGTCTA AAGAAAAGAA GTTCCAGCCT TGATGCCTTG
    GATGCTGACA GTGAAGGAGA GGGCCATTCG GAGCAGTCGC ACAATTGCTA CACTCCCGTG
    TGTCAGAGTT CCTCGAGAAC TGGCATTGCT AGCGGGGATG AATTGGACTC TTTTGAGACC
    AACACTGAAC CAGATTTTAA TATCTCCAGG ACGGAATCAT TTTCTTTATC AAGTAGCCTG
    CAGCTGAAGG AATCATTGTT TTCTGGAATC CGTTCACGTT CGTATTCCTG CTCATCACCC
    AAAATTTCTT TAGGAAAAAC TCGATTGATG CGTGATTTTA CAGTGTGTAA TTCAATTGAA
    GAGCAAAGAG CTTACAGCTT GCCGGAGCCA CCGAGAGAAC AAAGAATTCA GGAAGAAGAA
    TGGGATAAAT ATATCATACC TGCCAAATCA GAATCTGAAA AATACAAAGT AAGCCGAACT
    TTTAGCTTCC TCATGAATAG GATGACCAGC CCTCGGAATA AATCAAAGAC AAAAGGCAAG
    GATGCCAAAG ACAAAGAGAA ACTGACTCGA CATCAGTTTG TCCCTGGAAC TTTCTCTGGG
    GTTCTGCAGT GTTTGGTTTG CGATAAAACG CTCCTGGGGA AAGAGTCATT CCAGTGCTCC
    AACTGTAATG GAAACGTACA TAAAGTCTGT AAAGATGCTG CCCCTCCGTG TACCAAGAAA
    TTCCAAGACA AATATAATAA GAACAAACCA CAGACCATCC TTGGAAATTC TTCGTTTCGA
    GACGTCCCAT TGCCTGGGCT CTCTCTGCAC CCTTCCTCCT CCATGCCTAT CGGGTTGCCA
    ACTGGGAGGA AGGAGATGGC AGGGCAAGTG CATCCCTTGT CCAGAAGTGT CCCAGGCACC
    ACCTTGGAAA GCTTCAGGAG GCCGGGGGCA TCCTTGGAGT CCGAGAATGA TGGCAGCACC
    TGGAGAAGCA GGTCTCACTC TGACGAGCTG TTCCAGCCCA TGGGCTCGTC CCCCTCCACG
    GACTCCTTCT TAACGGAAGA TGTCGTGGAT TCTTCTCTGT GGAGTGACCT CAGCGGCGAT
    GCCCAGGAGT TTGAAGCGGA ATCTTGGAGT CTGGTGGTGG ACCCTTCATT TTGTAGTCGG
    CAGGAGAAGG ATGTCATCCA AAGACAGGAT GTCATTTTTG AGCTAATGCA GACAGAACTG
    CATCACATCC AGACCCTGTT CATCATGTCT GAGATCTTCA GGAAAGGGAT GAAAGAGGAG
    CTGCAGCTGG ACCACAGCAC TGTGGACAAA ATCTTCCCCT GTCTGGACGA GTTACTTGAA
    ATCCACAAGC ATTTCTTCTG TAGTATGAAG GAGCGAAGGC AGGAATCCTG TGTTGGCAAT
    GACAGGAATT TCATCATCGG CCGAATTGGA GATATTCTAG TACAACAGTT TTCAGAAGAA
    AATGCAAATA AAATGAAGAA AATATATGGG GAATTCTGTA GCCATCACAA AGAGGCTGTT
    AGCCTCTTTA AAGAACTCCA GCAGAATAAA AAGTTTCAGA ATTTTATTAA GCTCCGAAAT
    AGTAACCTTC TGGCTCGACG CCGAGGAATT CCAGAATGCA TTCTGCTGGT CACTCAGCGC
    ATCACAAAAT ACCCTGTCTT GGTGGAAAGG ATATTGCAGT ACACAAAGGA GAGCACTGAA
    GAACAGAGAG ACTTATGCAA AGCTCTTTGC TTAATTAAAG ACATGATTGC AGCTGTGGAT
    TTAAAAGTCA GCGAATATGA GAAAAAACAA AAATGGCTTG AGATCCTAAA TAAGATCGAA
    AACAAAACAT ACACCAAGCT CAAAAATGGC CATGTGTTTA GGAAACAGGC ACTGATGAGT
    AAAGAGAGGA CCCTGTTATA TGATGGCCTT GTCTATTGGA AAACTGCTAC AGGTCGTTTC
    AAAGATATCC TAGCTCTACT TCTAACTGAT GTGCTGCTCT TTTTACAAGA AAAAGACCAG
    AAATACATCT TTGCAGCTGT TGATCAGAAG CCATCAGTTA TTTCCCTTCA GAAGCTCATT
    GCTAGGGAAG TTGCGAATGA GGAGAGAGGA ATGTTTTTGA TCAGCGCTTC ATCTGCTGGT
    CCCGAGATGT ATGAAATCCA TACCAACTCC AAGGAGGAAA GAAATACCTG GATGAGACGG
    ATCCAACAGG CAGTAGAAAG TTGCCCAGAA GAAGAAGGGG GAAGGACAAG TGAGTCTGAT
    GAAGAGAGGA GGAAAGCTGA AGCCAGAGTG GCGAAGATTC AGCAATGCCA AGAAATACTC
    AGTAACCAAG ATCAACAAAT TTGCACATAT TTGGAAGAGA AACTGCATAT CTACGCGAAA
    CTTGGAGAAC TAAGTGGATT TCAGGATGTC CATCTAGAGC CCCACCTCCT CATTCAACCA
    GACCCCGGAG AGCCTCCCCA GGCAGCCTCA TTACTGGCCG CAGCACTGAA AGAGGCTGAG
    AGTCTGCAGG TGGCAGTGAA GGCTTCCCAG ATGAGCGATG CATGCCAGTC CCCCGAGGAA
    GGTTGTGGAG AACCTGCTTT GCCGGACGTG CTCCATTCTC AGGACGCACC AGGATCGTCT
    ACTGCTTCAC TAGTCACAGA AGGGACAGGA GGAAGAGGCT GTTGGGATGT GGATGCCGGG
    ACCCAGGGTG TGGGAACCGA CCTGGCGGTT TCTGATGCGG GGGAGAAGGT GGAATATAGA
    AGTTTCCCAG GTTCTTTGCA GTCAGAGATT ATACAAGCGA TACAGAATTT AACTCGTCTC
    TTATATAGCC TTCAGGCCGC CTTGACCATT CAGGATAGCC ACATTGAGAT CCACAGGCTG
    GTTCTCCAAC AGCGGGAGAG CCTGGCCCCG GGCCCCGCGT TCCGAGGCAG CCCCTTTCAG
    GACCAGGAGA AGTCCCGCAG CCTGGAGAAG CAGCGGGAGG AGCTGGCCGA TACCCACAAG
    CTGCAGCTCC AGTTCCAGCA AGACCAGCAC CGCTGGCTCC GCAGGTGCGA CCAGCAGCAG
    CGCGAGCAGG AGGCCCGGGC CAGCCGGCTG CAGGAGCGCG AGAGGGAGTG CCGGGCGCAG
    GAGGAGCTGC TGCTCAGGAG CCGGGGCGAG CTGGACCAGC AGCTCCAAGA CTACCAGCAG
    AGCCTGGAGC GCCTGCGCGA GGGCCAGCGC CTGGTGGAGA GGGAGCGGGA GAGGATGCGG
    GCCCAGCAGA GCCTGCTGCG CCACTGGAAG CACGGGCGGC AGAGCAGCCT TCCCGCCGCG
    TTTTCTCCAG GAGCCCTGGA GGTAATGGAA CTTAATCGAT CTGAGACTTT ACGTCATGAA
    AATTCATTCT TCATCAATGA AGCTTTAGTG CAAATGTCAC TCAACCCTTT GAACAAACCG
    CCGCCACCAG GTATCCATCA GGATGCGGCT TACCCCCCAG GCATACCTCA TTCTGATTTG
    GTAAGGACTA CTGAAAATCA AGTAGACCTC AAGACGGACG TTTCTCAACC CTCAGATATC
    AACCATGAAC TGTGGATAGC GGCTGGGGCC TGTCATCAGA TACCCCCTCT CCACCAAAGC
    AGCAAGGATT CTTGTAAAAA TGATGTGAAC ATCTCTCAGA CTGCATACCC ACCCCTCCAT
    GACTCAAATC CACACAGCCC TCGGCTGCAG ACGTTTATAG CAGAAGAAAA GCTAAATCTA
    CAGAGAGTGA CCAGACAAGA CGGGGAAACT GGAAATGGAG CAGAAGAAAA TATTGTTTAC
    CTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa228080
    Clone ID Related Accession (Same CDS sequence) XM_024981394.1
    Accession Version XM_024981394.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5358bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product rho guanine nucleotide exchange factor 28 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGAGGGTCA TTTTTGTTAG AAACACCCAA GATTGTCTTC TGTTTTCTCC TAGGTGGGAA 
    GAAGATGCGG AAACCATGGA GCTGAACTGC AGCGAAGTAC CTCTGTATGG GCAGATGACG
    GTCTGCGCGA AGTTTGGCAA AAATGTTTAT CTTCCTAAAG ATGCTGAGTT TTACTTTATT
    TATAATGGAT CTCATCAGAG ACACATCGTG ATTGCAGAGC GCACAGAAGA TAATGTTCTC
    CAGTCCAGCA TTCCAGGCCA CGGGCTTCAG GAGACGGTCA CCGTGTCTGT GTGCCTGTGC
    TCGGAAGGTT ACTCCCCGGT GACCATGGGC TCTGACTCCG TGACCTACGT GGACAACATG
    GCCTGCAGGC TGGCCCGTCT GCTGGTCACC CAGGCCGATC GCCTCACCGC CAGCAGCCAC
    CAGACCTTGC TGACCCCGTT TGCCCTGACG GCGGGGGCAT TGCCTGCTCT GGACGAGGAG
    CTCGTGCTGG CCCTGACTCA TCTGGAGTTG CCTCTGGGGT GGACCGTCTT GGGGAATTCT
    TCACTGGAAG TTTCTCTGCA TAGGGAGTCT CTTCTGCACC TGGCTATGAG ATGGGGCCTT
    GCTAAGCTTT CCCAGCTCCT TCTGTGTCTC CCTGGGGGAG CCCAGGCCCT GACTTTACCC
    AACGAGGAGG GTGCCACGCC ATTAGACTTG GCTTTACATG GTGGACATTC CAAGCTGGTT
    GAAGACATCA CAAACTTCCA GGGCAGGCGT TCCCCAGGTT TCTCCTGCGT GCAGCTCAGC
    GAAGATGCCT CTCTGCGGTA TATTCACTCA TCAGAAACTC TGACCCTGAC GCTTAACCAC
    ACAGCCGAGC ATTTGCTGGA GGCGGATATT AAACTCTTCA GAAAATACTT TTGGGATAGA
    GCCTTTCTCA TCAAGGCTCT TGAAGAGCAA GAAGGCACGT CAGAGGAGAA ACCAGATATG
    CCCTCCAGCG CTGCAGAAAC TGAAGAAGGT ATGCATGCTC CTTCCCCACC TGGGGAACTC
    AGAAAGTGCT CAGCATTCTC TAGTCAATTG CGAGTTGAAG TGTGTGGAGA AGGAGAGCAG
    GAGCCGATGG ACTCGGAGGC GAGAGTAGGT TGCTCTGGGG CCGCAGGGCC CAGAGTTCCT
    TCAGCAGCAC TGACCAAAGA GATTAAGAAT TCGGGGTGCA GCAGATCACC ACCCGGGAAG
    GAGGGTGTAA AGCGTGTGAA AAGCCTGGTG GTTCCACTGA ATGAACATGA GGACCAGGAG
    AGGCTAGATT TGGATCGTTC CTTTGATGCC CTCAAAAAAT CCAAGCACCC CTCAACATTG
    CTTGCTGCTG GCCAGCTCTC TGACATGCTG AATGGTGGTG ATGACGTCTA CGCTAACTGC
    ATGGTGATAG ACCAGGTTGG TGATTTGGAC ATTAACTATA TTAATATAGA GGAAATCCCT
    GCAAATACCT GTCTTGAATC CATGGATTCT TCTCTGGGGC CTGAGAGCAG CAAACCCAGC
    CTTCCTACTG AAGCCAGTGC TGGCACATAC CCACCAAATA AGAGGCCAGA AGTGACGTCA
    CACACGGAAG CCCAATCATC TTTCATATCA TCTAGTTCGT ATGCTTCCAA TTTGAATCTT
    TCTTTTGGTC TTCGTGGATT TGAAAAGGAG CAAAGTCGTC TAAAGAAAAG AAGTTCCAGC
    CTTGATGCCT TGGATGCTGA CAGTGAAGGA GAGGGCCATT CGGAGCAGTC GCACAATTGC
    TACACTCCCG TGTGTCAGAG TTCCTCGAGA ACTGGCATTG CTAGCGGGGA TGAATTGGAC
    TCTTTTGAGA CCAACACTGA ACCAGATTTT AATATCTCCA GGACGGAATC ATTTTCTTTA
    TCAAGTAGCC TGCAGCTGAA GGAATCATTG TTTTCTGGAA TCCGTTCACG TTCGTATTCC
    TGCTCATCAC CCAAAATTTC TTTAGGAAAA ACTCGATTGA TGCGTGATTT TACAGTGTGT
    AATTCAATTG AAGAGCAAAG AGCTTACAGC TTGCCGGAGC CACCGAGAGA ACAAAGAATT
    CAGGAAGAAG AATGGGATAA ATATATCATA CCTGCCAAAT CAGAATCTGA AAAATACAAA
    GTAAGCCGAA CTTTTAGCTT CCTCATGAAT AGGATGACCA GCCCTCGGAA TAAATCAAAG
    ACAAAAGGCA AGGATGCCAA AGACAAAGAG AAACTGACTC GACATCAGTT TGTCCCTGGA
    ACTTTCTCTG GGGTTCTGCA GTGTTTGGTT TGCGATAAAA CGCTCCTGGG GAAAGAGTCA
    TTCCAGTGCT CCAACTGTAA TGGAAACGTA CATAAAGTCT GTAAAGATGC TGCCCCTCCG
    TGTACCAAGA AATTCCAAGA CAAATATAAT AAGAACAAAC CACAGACCAT CCTTGGAAAT
    TCTTCGTTTC GAGACGTCCC ATTGCCTGGG CTCTCTCTGC ACCCTTCCTC CTCCATGCCT
    ATCGGGTTGC CAACTGGGAG GAAGGAGATG GCAGGGCAAG TGCATCCCTT GTCCAGAAGT
    GTCCCAGGCA CCACCTTGGA AAGCTTCAGG AGGCCGGGGG CATCCTTGGA GTCCGAGAAT
    GATGGCAGCA CCTGGAGAAG CAGGTCTCAC TCTGACGAGC TGTTCCAGCC CATGGGCTCG
    TCCCCCTCCA CGGACTCCTT CTTAACGGAA GATGTCGTGG ATTCTTCTCT GTGGAGTGAC
    CTCAGCGGCG ATGCCCAGGA GTTTGAAGCG GAATCTTGGA GTCTGGTGGT GGACCCTTCA
    TTTTGTAGTC GGCAGGAGAA GGATGTCATC CAAAGACAGG ATGTCATTTT TGAGCTAATG
    CAGACAGAAC TGCATCACAT CCAGACCCTG TTCATCATGT CTGAGATCTT CAGGAAAGGG
    ATGAAAGAGG AGCTGCAGCT GGACCACAGC ACTGTGGACA AAATCTTCCC CTGTCTGGAC
    GAGTTACTTG AAATCCACAA GCATTTCTTC TGTAGTATGA AGGAGCGAAG GCAGGAATCC
    TGTGTTGGCA ATGACAGGAA TTTCATCATC GGCCGAATTG GAGATATTCT AGTACAACAG
    TTTTCAGAAG AAAATGCAAA TAAAATGAAG AAAATATATG GGGAATTCTG TAGCCATCAC
    AAAGAGGCTG TTAGCCTCTT TAAAGAACTC CAGCAGAATA AAAAGTTTCA GAATTTTATT
    AAGCTCCGAA ATAGTAACCT TCTGGCTCGA CGCCGAGGAA TTCCAGAATG CATTCTGCTG
    GTCACTCAGC GCATCACAAA ATACCCTGTC TTGGTGGAAA GGATATTGCA GTACACAAAG
    GAGAGCACTG AAGAACAGAG AGACTTATGC AAAGCTCTTT GCTTAATTAA AGACATGATT
    GCAGCTGTGG ATTTAAAAGT CAGCGAATAT GAGAAAAAAC AAAAATGGCT TGAGATCCTA
    AATAAGATCG AAAACAAAAC ATACACCAAG CTCAAAAATG GCCATGTGTT TAGGAAACAG
    GCACTGATGA GTAAAGAGAG GACCCTGTTA TATGATGGCC TTGTCTATTG GAAAACTGCT
    ACAGGTCGTT TCAAAGATAT CCTAGCTCTA CTTCTAACTG ATGTGCTGCT CTTTTTACAA
    GAAAAAGACC AGAAATACAT CTTTGCAGCT GTTGATCAGA AGCCATCAGT TATTTCCCTT
    CAGAAGCTCA TTGCTAGGGA AGTTGCGAAT GAGGAGAGAG GAATGTTTTT GATCAGCGCT
    TCATCTGCTG GTCCCGAGAT GTATGAAATC CATACCAACT CCAAGGAGGA AAGAAATACC
    TGGATGAGAC GGATCCAACA GGCAGTAGAA AGTTGCCCAG AAGAAGAAGG GGGAAGGACA
    AGTGAGTCTG ATGAAGAGAG GAGGAAAGCT GAAGCCAGAG TGGCGAAGAT TCAGCAATGC
    CAAGAAATAC TCAGTAACCA AGATCAACAA ATTTGCACAT ATTTGGAAGA GAAACTGCAT
    ATCTACGCGA AACTTGGAGA ACTAAGTGGA TTTCAGGATG TCCATCTAGA GCCCCACCTC
    CTCATTCAAC CAGACCCCGG AGAGCCTCCC CAGGCAGCCT CATTACTGGC CGCAGCACTG
    AAAGAGGCTG AGAGTCTGCA GGTGGCAGTG AAGGCTTCCC AGATGAGCGA TGCATGCCAG
    TCCCCCGAGG AAGGTTGTGG AGAACCTGCT TTGCCGGACG TGCTCCATTC TCAGGACGCA
    CCAGGATCGT CTACTGCTTC ACTAGTCACA GAAGGGACAG GAGGAAGAGG CTGTTGGGAT
    GTGGATGCCG GGACCCAGGG TGTGGGAACC GACCTGGCGG TTTCTGATGC GGGGGAGAAG
    GTGGAATATA GAAGTTTCCC AGGTTCTTTG CAGTCAGAGA TTATACAAGC GATACAGAAT
    TTAACTCGTC TCTTATATAG CCTTCAGGCC GCCTTGACCA TTCAGGATAG CCACATTGAG
    ATCCACAGGC TGGTTCTCCA ACAGCGGGAG AGCCTGGCCC CGGGCCCCGC GTTCCGAGGC
    AGCCCCTTTC AGGACCAGGA GAAGTCCCGC AGCCTGGAGA AGCAGCGGGA GGAGCTGGCC
    GATACCCACA AGCTGCAGCT CCAGTTCCAG CAAGACCAGC ACCGCTGGCT CCGCAGGTGC
    GACCAGCAGC AGCGCGAGCA GGAGGCCCGG GCCAGCCGGC TGCAGGAGCG CGAGAGGGAG
    TGCCGGGCGC AGGAGGAGCT GCTGCTCAGG AGCCGGGGCG AGCTGGACCA GCAGCTCCAA
    GACTACCAGC AGAGCCTGGA GCGCCTGCGC GAGGGCCAGC GCCTGGTGGA GAGGGAGCGG
    GAGAGGATGC GGGCCCAGCA GAGCCTGCTG CGCCACTGGA AGCACGGGCG GCAGAGCAGC
    CTTCCCGCCG CGTTTTCTCC AGGAGCCCTG GAGGTAATGG AACTTAATCG ATCTGAGACT
    TTACGTCATG AAAATTCATT CTTCATCAAT GAAGCTTTAG TGCAAATGTC ACTCAACCCT
    TTGAACAAAC CGCCGCCACC AGGTATCCAT CAGGATGCGG CTTACCCCCC AGGCATACCT
    CATTCTGATT TGGTAAGGAC TACTGAAAAT CAAGTAGACC TCAAGACGGA CGTTTCTCAA
    CCCTCAGATA TCAACCATGA ACTGTGGATA GCGGCTGGGG CCTGTCATCA GATACCCCCT
    CTCCACCAAA GCAGCAAGGA TTCTTGTAAA AATGATGTGA ACATCTCTCA GACTGCATAC
    CCACCCCTCC ATGACTCAAA TCCACACAGC CCTCGGCTGC AGACGTTTAT AGCAGAAGAA
    AAGCTAAATC TACAGAGAGT GACCAGACAA GACGGGGAAA CTGGAAATGG AGCAGAAGAA
    AATATTGTTT ACCTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024837162.1
    CDS102..5459
    Translation

    Target ORF information:

    RefSeq Version XM_024981394.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981394.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGAGGGTCA TTTTTGTTAG AAACACCCAA GATTGTCTTC TGTTTTCTCC TAGGTGGGAA 
    GAAGATGCGG AAACCATGGA GCTGAACTGC AGCGAAGTAC CTCTGTATGG GCAGATGACG
    GTCTGCGCGA AGTTTGGCAA AAATGTTTAT CTTCCTAAAG ATGCTGAGTT TTACTTTATT
    TATAATGGAT CTCATCAGAG ACACATCGTG ATTGCAGAGC GCACAGAAGA TAATGTTCTC
    CAGTCCAGCA TTCCAGGCCA CGGGCTTCAG GAGACGGTCA CCGTGTCTGT GTGCCTGTGC
    TCGGAAGGTT ACTCCCCGGT GACCATGGGC TCTGACTCCG TGACCTACGT GGACAACATG
    GCCTGCAGGC TGGCCCGTCT GCTGGTCACC CAGGCCGATC GCCTCACCGC CAGCAGCCAC
    CAGACCTTGC TGACCCCGTT TGCCCTGACG GCGGGGGCAT TGCCTGCTCT GGACGAGGAG
    CTCGTGCTGG CCCTGACTCA TCTGGAGTTG CCTCTGGGGT GGACCGTCTT GGGGAATTCT
    TCACTGGAAG TTTCTCTGCA TAGGGAGTCT CTTCTGCACC TGGCTATGAG ATGGGGCCTT
    GCTAAGCTTT CCCAGCTCCT TCTGTGTCTC CCTGGGGGAG CCCAGGCCCT GACTTTACCC
    AACGAGGAGG GTGCCACGCC ATTAGACTTG GCTTTACATG GTGGACATTC CAAGCTGGTT
    GAAGACATCA CAAACTTCCA GGGCAGGCGT TCCCCAGGTT TCTCCTGCGT GCAGCTCAGC
    GAAGATGCCT CTCTGCGGTA TATTCACTCA TCAGAAACTC TGACCCTGAC GCTTAACCAC
    ACAGCCGAGC ATTTGCTGGA GGCGGATATT AAACTCTTCA GAAAATACTT TTGGGATAGA
    GCCTTTCTCA TCAAGGCTCT TGAAGAGCAA GAAGGCACGT CAGAGGAGAA ACCAGATATG
    CCCTCCAGCG CTGCAGAAAC TGAAGAAGGT ATGCATGCTC CTTCCCCACC TGGGGAACTC
    AGAAAGTGCT CAGCATTCTC TAGTCAATTG CGAGTTGAAG TGTGTGGAGA AGGAGAGCAG
    GAGCCGATGG ACTCGGAGGC GAGAGTAGGT TGCTCTGGGG CCGCAGGGCC CAGAGTTCCT
    TCAGCAGCAC TGACCAAAGA GATTAAGAAT TCGGGGTGCA GCAGATCACC ACCCGGGAAG
    GAGGGTGTAA AGCGTGTGAA AAGCCTGGTG GTTCCACTGA ATGAACATGA GGACCAGGAG
    AGGCTAGATT TGGATCGTTC CTTTGATGCC CTCAAAAAAT CCAAGCACCC CTCAACATTG
    CTTGCTGCTG GCCAGCTCTC TGACATGCTG AATGGTGGTG ATGACGTCTA CGCTAACTGC
    ATGGTGATAG ACCAGGTTGG TGATTTGGAC ATTAACTATA TTAATATAGA GGAAATCCCT
    GCAAATACCT GTCTTGAATC CATGGATTCT TCTCTGGGGC CTGAGAGCAG CAAACCCAGC
    CTTCCTACTG AAGCCAGTGC TGGCACATAC CCACCAAATA AGAGGCCAGA AGTGACGTCA
    CACACGGAAG CCCAATCATC TTTCATATCA TCTAGTTCGT ATGCTTCCAA TTTGAATCTT
    TCTTTTGGTC TTCGTGGATT TGAAAAGGAG CAAAGTCGTC TAAAGAAAAG AAGTTCCAGC
    CTTGATGCCT TGGATGCTGA CAGTGAAGGA GAGGGCCATT CGGAGCAGTC GCACAATTGC
    TACACTCCCG TGTGTCAGAG TTCCTCGAGA ACTGGCATTG CTAGCGGGGA TGAATTGGAC
    TCTTTTGAGA CCAACACTGA ACCAGATTTT AATATCTCCA GGACGGAATC ATTTTCTTTA
    TCAAGTAGCC TGCAGCTGAA GGAATCATTG TTTTCTGGAA TCCGTTCACG TTCGTATTCC
    TGCTCATCAC CCAAAATTTC TTTAGGAAAA ACTCGATTGA TGCGTGATTT TACAGTGTGT
    AATTCAATTG AAGAGCAAAG AGCTTACAGC TTGCCGGAGC CACCGAGAGA ACAAAGAATT
    CAGGAAGAAG AATGGGATAA ATATATCATA CCTGCCAAAT CAGAATCTGA AAAATACAAA
    GTAAGCCGAA CTTTTAGCTT CCTCATGAAT AGGATGACCA GCCCTCGGAA TAAATCAAAG
    ACAAAAGGCA AGGATGCCAA AGACAAAGAG AAACTGACTC GACATCAGTT TGTCCCTGGA
    ACTTTCTCTG GGGTTCTGCA GTGTTTGGTT TGCGATAAAA CGCTCCTGGG GAAAGAGTCA
    TTCCAGTGCT CCAACTGTAA TGGAAACGTA CATAAAGTCT GTAAAGATGC TGCCCCTCCG
    TGTACCAAGA AATTCCAAGA CAAATATAAT AAGAACAAAC CACAGACCAT CCTTGGAAAT
    TCTTCGTTTC GAGACGTCCC ATTGCCTGGG CTCTCTCTGC ACCCTTCCTC CTCCATGCCT
    ATCGGGTTGC CAACTGGGAG GAAGGAGATG GCAGGGCAAG TGCATCCCTT GTCCAGAAGT
    GTCCCAGGCA CCACCTTGGA AAGCTTCAGG AGGCCGGGGG CATCCTTGGA GTCCGAGAAT
    GATGGCAGCA CCTGGAGAAG CAGGTCTCAC TCTGACGAGC TGTTCCAGCC CATGGGCTCG
    TCCCCCTCCA CGGACTCCTT CTTAACGGAA GATGTCGTGG ATTCTTCTCT GTGGAGTGAC
    CTCAGCGGCG ATGCCCAGGA GTTTGAAGCG GAATCTTGGA GTCTGGTGGT GGACCCTTCA
    TTTTGTAGTC GGCAGGAGAA GGATGTCATC CAAAGACAGG ATGTCATTTT TGAGCTAATG
    CAGACAGAAC TGCATCACAT CCAGACCCTG TTCATCATGT CTGAGATCTT CAGGAAAGGG
    ATGAAAGAGG AGCTGCAGCT GGACCACAGC ACTGTGGACA AAATCTTCCC CTGTCTGGAC
    GAGTTACTTG AAATCCACAA GCATTTCTTC TGTAGTATGA AGGAGCGAAG GCAGGAATCC
    TGTGTTGGCA ATGACAGGAA TTTCATCATC GGCCGAATTG GAGATATTCT AGTACAACAG
    TTTTCAGAAG AAAATGCAAA TAAAATGAAG AAAATATATG GGGAATTCTG TAGCCATCAC
    AAAGAGGCTG TTAGCCTCTT TAAAGAACTC CAGCAGAATA AAAAGTTTCA GAATTTTATT
    AAGCTCCGAA ATAGTAACCT TCTGGCTCGA CGCCGAGGAA TTCCAGAATG CATTCTGCTG
    GTCACTCAGC GCATCACAAA ATACCCTGTC TTGGTGGAAA GGATATTGCA GTACACAAAG
    GAGAGCACTG AAGAACAGAG AGACTTATGC AAAGCTCTTT GCTTAATTAA AGACATGATT
    GCAGCTGTGG ATTTAAAAGT CAGCGAATAT GAGAAAAAAC AAAAATGGCT TGAGATCCTA
    AATAAGATCG AAAACAAAAC ATACACCAAG CTCAAAAATG GCCATGTGTT TAGGAAACAG
    GCACTGATGA GTAAAGAGAG GACCCTGTTA TATGATGGCC TTGTCTATTG GAAAACTGCT
    ACAGGTCGTT TCAAAGATAT CCTAGCTCTA CTTCTAACTG ATGTGCTGCT CTTTTTACAA
    GAAAAAGACC AGAAATACAT CTTTGCAGCT GTTGATCAGA AGCCATCAGT TATTTCCCTT
    CAGAAGCTCA TTGCTAGGGA AGTTGCGAAT GAGGAGAGAG GAATGTTTTT GATCAGCGCT
    TCATCTGCTG GTCCCGAGAT GTATGAAATC CATACCAACT CCAAGGAGGA AAGAAATACC
    TGGATGAGAC GGATCCAACA GGCAGTAGAA AGTTGCCCAG AAGAAGAAGG GGGAAGGACA
    AGTGAGTCTG ATGAAGAGAG GAGGAAAGCT GAAGCCAGAG TGGCGAAGAT TCAGCAATGC
    CAAGAAATAC TCAGTAACCA AGATCAACAA ATTTGCACAT ATTTGGAAGA GAAACTGCAT
    ATCTACGCGA AACTTGGAGA ACTAAGTGGA TTTCAGGATG TCCATCTAGA GCCCCACCTC
    CTCATTCAAC CAGACCCCGG AGAGCCTCCC CAGGCAGCCT CATTACTGGC CGCAGCACTG
    AAAGAGGCTG AGAGTCTGCA GGTGGCAGTG AAGGCTTCCC AGATGAGCGA TGCATGCCAG
    TCCCCCGAGG AAGGTTGTGG AGAACCTGCT TTGCCGGACG TGCTCCATTC TCAGGACGCA
    CCAGGATCGT CTACTGCTTC ACTAGTCACA GAAGGGACAG GAGGAAGAGG CTGTTGGGAT
    GTGGATGCCG GGACCCAGGG TGTGGGAACC GACCTGGCGG TTTCTGATGC GGGGGAGAAG
    GTGGAATATA GAAGTTTCCC AGGTTCTTTG CAGTCAGAGA TTATACAAGC GATACAGAAT
    TTAACTCGTC TCTTATATAG CCTTCAGGCC GCCTTGACCA TTCAGGATAG CCACATTGAG
    ATCCACAGGC TGGTTCTCCA ACAGCGGGAG AGCCTGGCCC CGGGCCCCGC GTTCCGAGGC
    AGCCCCTTTC AGGACCAGGA GAAGTCCCGC AGCCTGGAGA AGCAGCGGGA GGAGCTGGCC
    GATACCCACA AGCTGCAGCT CCAGTTCCAG CAAGACCAGC ACCGCTGGCT CCGCAGGTGC
    GACCAGCAGC AGCGCGAGCA GGAGGCCCGG GCCAGCCGGC TGCAGGAGCG CGAGAGGGAG
    TGCCGGGCGC AGGAGGAGCT GCTGCTCAGG AGCCGGGGCG AGCTGGACCA GCAGCTCCAA
    GACTACCAGC AGAGCCTGGA GCGCCTGCGC GAGGGCCAGC GCCTGGTGGA GAGGGAGCGG
    GAGAGGATGC GGGCCCAGCA GAGCCTGCTG CGCCACTGGA AGCACGGGCG GCAGAGCAGC
    CTTCCCGCCG CGTTTTCTCC AGGAGCCCTG GAGGTAATGG AACTTAATCG ATCTGAGACT
    TTACGTCATG AAAATTCATT CTTCATCAAT GAAGCTTTAG TGCAAATGTC ACTCAACCCT
    TTGAACAAAC CGCCGCCACC AGGTATCCAT CAGGATGCGG CTTACCCCCC AGGCATACCT
    CATTCTGATT TGGTAAGGAC TACTGAAAAT CAAGTAGACC TCAAGACGGA CGTTTCTCAA
    CCCTCAGATA TCAACCATGA ACTGTGGATA GCGGCTGGGG CCTGTCATCA GATACCCCCT
    CTCCACCAAA GCAGCAAGGA TTCTTGTAAA AATGATGTGA ACATCTCTCA GACTGCATAC
    CCACCCCTCC ATGACTCAAA TCCACACAGC CCTCGGCTGC AGACGTTTAT AGCAGAAGAA
    AAGCTAAATC TACAGAGAGT GACCAGACAA GACGGGGAAA CTGGAAATGG AGCAGAAGAA
    AATATTGTTT ACCTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa228081
    Clone ID Related Accession (Same CDS sequence) XM_024981395.1
    Accession Version XM_024981395.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5301bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product rho guanine nucleotide exchange factor 28 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGCAGCCCA CTCTTCACTT TCTTCCCATC TTTAGGTGTC CGTTCAGCTG TGAGCTCTGT 
    GAGGAGTGGA TCGTTTCCTG GTCATCTTTG TGCAATGCTG AGCCCATTGA AGATGCGGAA
    ACCATGGAGC TGAACTGCAG CGAAGTACCT CTGTATGGGC AGATGACGGT CTGCGCGAAG
    TTTGGCAAAA ATGTTTATCT TCCTAAAGAT GCTGAGTTTT ACTTTATTTA TAATGGATCT
    CATCAGAGAC ACATCGTGAT TGCAGAGCGC ACAGAAGATA ATGTTCTCCA GTCCAGCATT
    CCAGGCCACG GGCTTCAGGA GACGGTCACC GTGTCTGTGT GCCTGTGCTC GGAAGGTTAC
    TCCCCGGTGA CCATGGGCTC TGACTCCGTG ACCTACGTGG ACAACATGGC CTGCAGGCTG
    GCCCGTCTGC TGGTCACCCA GGCCGATCGC CTCACCGCCA GCAGCCACCA GACCTTGCTG
    ACCCCGTTTG CCCTGACGGC GGGGGCATTG CCTGCTCTGG ACGAGGAGCT CGTGCTGGCC
    CTGACTCATC TGGAGTTGCC TCTGGGGTGG ACCGTCTTGG GGAATTCTTC ACTGGAAGTT
    TCTCTGCATA GGGAGTCTCT TCTGCACCTG GCTATGAGAT GGGGCCTTGC TAAGCTTTCC
    CAGCTCCTTC TGTGTCTCCC TGGGGGAGCC CAGGCCCTGA CTTTACCCAA CGAGGAGGGT
    GCCACGCCAT TAGACTTGGC TTTACATGGT GGACATTCCA AGCTGGTTGA AGACATCACA
    AACTTCCAGG GCAGGCGTTC CCCAGGTTTC TCCTGCGTGC AGCTCAGCGA AGATGCCTCT
    CTGCGGTATA TTCACTCATC AGAAACTCTG ACCCTGACGC TTAACCACAC AGCCGAGCAT
    TTGCTGGAGG CGGATATTAA ACTCTTCAGA AAATACTTTT GGGATAGAGC CTTTCTCATC
    AAGGCTCTTG AAGAGCAAGA AGGCACGTCA GAGGAGAAAC CAGATATGCC CTCCAGCGCT
    GCAGAAACTG AAGAAGGTAT GCATGCTCCT TCCCCACCTG GGGAACTCAG AAAGTGCTCA
    GCATTCTCTA GTCAATTGCG AGTTGAAGTG TGTGGAGAAG GAGAGCAGGA GCCGATGGAC
    TCGGAGGCGA GAGTAGGTTG CTCTGGGGCC GCAGGGCCCA GAGTTCCTTC AGCAGCACTG
    ACCAAAGAGA TTAAGAATTC GGGGTGCAGC AGATCACCAC CCGGGAAGGA GGGTGTAAAG
    CGTGTGAAAA GCCTGGTGGT TCCACTGAAT GAACATGAGG ACCAGGAGAG GCTAGATTTG
    GATCGTTCCT TTGATGCCCT CAAAAAATCC AAGCACCCCT CAACATTGCT TGCTGCTGGC
    CAGCTCTCTG ACATGCTGAA TGGTGGTGAT GACGTCTACG CTAACTGCAT GGTGATAGAC
    CAGGTTGGTG ATTTGGACAT TAACTATATT AATATAGAGG AAATCCCTGC AAATACCTGT
    CTTGAATCCA TGGATTCTTC TCTGGGGCCT GAGAGCAGCA AACCCAGCCT TCCTACTGAA
    GCCAGTGCTG GCACATACCC ACCAAATAAG AGGCCAGAAG TGACGTCACA CACGGAAGCC
    CAATCATCTT TCATATCATC TAGTTCGTAT GCTTCCAATT TGAATCTTTC TTTTGGTCTT
    CGTGGATTTG AAAAGGAGCA AAGTCGTCTA AAGAAAAGAA GTTCCAGCCT TGATGCCTTG
    GATGCTGACA GTGAAGGAGA GGGCCATTCG GAGCAGTCGC ACAATTGCTA CACTCCCGTG
    TGTCAGAGTT CCTCGAGAAC TGGCATTGCT AGCGGGGATG AATTGGACTC TTTTGAGACC
    AACACTGAAC CAGATTTTAA TATCTCCAGG ACGGAATCAT TTTCTTTATC AAGTAGCCTG
    CAGCTGAAGG AATCATTGTT TTCTGGAATC CGTTCACGTT CGTATTCCTG CTCATCACCC
    AAAATTTCTT TAGGAAAAAC TCGATTGATG CGTGATTTTA CAGTGTGTAA TTCAATTGAA
    GAGCAAAGAG CTTACAGCTT GCCGGAGCCA CCGAGAGAAC AAAGAATTCA GGAAGAAGAA
    TGGGATAAAT ATATCATACC TGCCAAATCA GAATCTGAAA AATACAAAGT AAGCCGAACT
    TTTAGCTTCC TCATGAATAG GATGACCAGC CCTCGGAATA AATCAAAGAC AAAAGGCAAG
    GATGCCAAAG ACAAAGAGAA ACTGACTCGA CATCAGTTTG TCCCTGGAAC TTTCTCTGGG
    GTTCTGCAGT GTTTGGTTTG CGATAAAACG CTCCTGGGGA AAGAGTCATT CCAGTGCTCC
    AATTCTTCGT TTCGAGACGT CCCATTGCCT GGGCTCTCTC TGCACCCTTC CTCCTCCATG
    CCTATCGGGT TGCCAACTGG GAGGAAGGAG ATGGCAGGGC AAGTGCATCC CTTGTCCAGA
    AGTGTCCCAG GCACCACCTT GGAAAGCTTC AGGAGGCCGG GGGCATCCTT GGAGTCCGAG
    AATGATGGCA GCACCTGGAG AAGCAGGTCT CACTCTGACG AGCTGTTCCA GCCCATGGGC
    TCGTCCCCCT CCACGGACTC CTTCTTAACG GAAGATGTCG TGGATTCTTC TCTGTGGAGT
    GACCTCAGCG GCGATGCCCA GGAGTTTGAA GCGGAATCTT GGAGTCTGGT GGTGGACCCT
    TCATTTTGTA GTCGGCAGGA GAAGGATGTC ATCCAAAGAC AGGATGTCAT TTTTGAGCTA
    ATGCAGACAG AACTGCATCA CATCCAGACC CTGTTCATCA TGTCTGAGAT CTTCAGGAAA
    GGGATGAAAG AGGAGCTGCA GCTGGACCAC AGCACTGTGG ACAAAATCTT CCCCTGTCTG
    GACGAGTTAC TTGAAATCCA CAAGCATTTC TTCTGTAGTA TGAAGGAGCG AAGGCAGGAA
    TCCTGTGTTG GCAATGACAG GAATTTCATC ATCGGCCGAA TTGGAGATAT TCTAGTACAA
    CAGTTTTCAG AAGAAAATGC AAATAAAATG AAGAAAATAT ATGGGGAATT CTGTAGCCAT
    CACAAAGAGG CTGTTAGCCT CTTTAAAGAA CTCCAGCAGA ATAAAAAGTT TCAGAATTTT
    ATTAAGCTCC GAAATAGTAA CCTTCTGGCT CGACGCCGAG GAATTCCAGA ATGCATTCTG
    CTGGTCACTC AGCGCATCAC AAAATACCCT GTCTTGGTGG AAAGGATATT GCAGTACACA
    AAGGAGAGCA CTGAAGAACA GAGAGACTTA TGCAAAGCTC TTTGCTTAAT TAAAGACATG
    ATTGCAGCTG TGGATTTAAA AGTCAGCGAA TATGAGAAAA AACAAAAATG GCTTGAGATC
    CTAAATAAGA TCGAAAACAA AACATACACC AAGCTCAAAA ATGGCCATGT GTTTAGGAAA
    CAGGCACTGA TGAGTAAAGA GAGGACCCTG TTATATGATG GCCTTGTCTA TTGGAAAACT
    GCTACAGGTC GTTTCAAAGA TATCCTAGCT CTACTTCTAA CTGATGTGCT GCTCTTTTTA
    CAAGAAAAAG ACCAGAAATA CATCTTTGCA GCTGTTGATC AGAAGCCATC AGTTATTTCC
    CTTCAGAAGC TCATTGCTAG GGAAGTTGCG AATGAGGAGA GAGGAATGTT TTTGATCAGC
    GCTTCATCTG CTGGTCCCGA GATGTATGAA ATCCATACCA ACTCCAAGGA GGAAAGAAAT
    ACCTGGATGA GACGGATCCA ACAGGCAGTA GAAAGTTGCC CAGAAGAAGA AGGGGGAAGG
    ACAAGTGAGT CTGATGAAGA GAGGAGGAAA GCTGAAGCCA GAGTGGCGAA GATTCAGCAA
    TGCCAAGAAA TACTCAGTAA CCAAGATCAA CAAATTTGCA CATATTTGGA AGAGAAACTG
    CATATCTACG CGAAACTTGG AGAACTAAGT GGATTTCAGG ATGTCCATCT AGAGCCCCAC
    CTCCTCATTC AACCAGACCC CGGAGAGCCT CCCCAGGCAG CCTCATTACT GGCCGCAGCA
    CTGAAAGAGG CTGAGAGTCT GCAGGTGGCA GTGAAGGCTT CCCAGATGAG CGATGCATGC
    CAGTCCCCCG AGGAAGGTTG TGGAGAACCT GCTTTGCCGG ACGTGCTCCA TTCTCAGGAC
    GCACCAGGAT CGTCTACTGC TTCACTAGTC ACAGAAGGGA CAGGAGGAAG AGGCTGTTGG
    GATGTGGATG CCGGGACCCA GGGTGTGGGA ACCGACCTGG CGGTTTCTGA TGCGGGGGAG
    AAGGTGGAAT ATAGAAGTTT CCCAGGTTCT TTGCAGTCAG AGATTATACA AGCGATACAG
    AATTTAACTC GTCTCTTATA TAGCCTTCAG GCCGCCTTGA CCATTCAGGA TAGCCACATT
    GAGATCCACA GGCTGGTTCT CCAACAGCGG GAGAGCCTGG CCCCGGGCCC CGCGTTCCGA
    GGCAGCCCCT TTCAGGACCA GGAGAAGTCC CGCAGCCTGG AGAAGCAGCG GGAGGAGCTG
    GCCGATACCC ACAAGCTGCA GCTCCAGTTC CAGCAAGACC AGCACCGCTG GCTCCGCAGG
    TGCGACCAGC AGCAGCGCGA GCAGGAGGCC CGGGCCAGCC GGCTGCAGGA GCGCGAGAGG
    GAGTGCCGGG CGCAGGAGGA GCTGCTGCTC AGGAGCCGGG GCGAGCTGGA CCAGCAGCTC
    CAAGACTACC AGCAGAGCCT GGAGCGCCTG CGCGAGGGCC AGCGCCTGGT GGAGAGGGAG
    CGGGAGAGGA TGCGGGCCCA GCAGAGCCTG CTGCGCCACT GGAAGCACGG GCGGCAGAGC
    AGCCTTCCCG CCGCGTTTTC TCCAGGAGCC CTGGAGGTAA TGGAACTTAA TCGATCTGAG
    ACTTTACGTC ATGAAAATTC ATTCTTCATC AATGAAGCTT TAGTGCAAAT GTCACTCAAC
    CCTTTGAACA AACCGCCGCC ACCAGGTATC CATCAGGATG CGGCTTACCC CCCAGGCATA
    CCTCATTCTG ATTTGGTAAG GACTACTGAA AATCAAGTAG ACCTCAAGAC GGACGTTTCT
    CAACCCTCAG ATATCAACCA TGAACTGTGG ATAGCGGCTG GGGCCTGTCA TCAGATACCC
    CCTCTCCACC AAAGCAGCAA GGATTCTTGT AAAAATGATG TGAACATCTC TCAGACTGCA
    TACCCACCCC TCCATGACTC AAATCCACAC AGCCCTCGGC TGCAGACGTT TATAGCAGAA
    GAAAAGCTAA ATCTACAGAG AGTGACCAGA CAAGACGGGG AAACTGGAAA TGGAGCAGAA
    GAAAATATTG TTTACCTCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024837163.1
    CDS118..5418
    Translation

    Target ORF information:

    RefSeq Version XM_024981395.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981395.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGCAGCCCA CTCTTCACTT TCTTCCCATC TTTAGGTGTC CGTTCAGCTG TGAGCTCTGT 
    GAGGAGTGGA TCGTTTCCTG GTCATCTTTG TGCAATGCTG AGCCCATTGA AGATGCGGAA
    ACCATGGAGC TGAACTGCAG CGAAGTACCT CTGTATGGGC AGATGACGGT CTGCGCGAAG
    TTTGGCAAAA ATGTTTATCT TCCTAAAGAT GCTGAGTTTT ACTTTATTTA TAATGGATCT
    CATCAGAGAC ACATCGTGAT TGCAGAGCGC ACAGAAGATA ATGTTCTCCA GTCCAGCATT
    CCAGGCCACG GGCTTCAGGA GACGGTCACC GTGTCTGTGT GCCTGTGCTC GGAAGGTTAC
    TCCCCGGTGA CCATGGGCTC TGACTCCGTG ACCTACGTGG ACAACATGGC CTGCAGGCTG
    GCCCGTCTGC TGGTCACCCA GGCCGATCGC CTCACCGCCA GCAGCCACCA GACCTTGCTG
    ACCCCGTTTG CCCTGACGGC GGGGGCATTG CCTGCTCTGG ACGAGGAGCT CGTGCTGGCC
    CTGACTCATC TGGAGTTGCC TCTGGGGTGG ACCGTCTTGG GGAATTCTTC ACTGGAAGTT
    TCTCTGCATA GGGAGTCTCT TCTGCACCTG GCTATGAGAT GGGGCCTTGC TAAGCTTTCC
    CAGCTCCTTC TGTGTCTCCC TGGGGGAGCC CAGGCCCTGA CTTTACCCAA CGAGGAGGGT
    GCCACGCCAT TAGACTTGGC TTTACATGGT GGACATTCCA AGCTGGTTGA AGACATCACA
    AACTTCCAGG GCAGGCGTTC CCCAGGTTTC TCCTGCGTGC AGCTCAGCGA AGATGCCTCT
    CTGCGGTATA TTCACTCATC AGAAACTCTG ACCCTGACGC TTAACCACAC AGCCGAGCAT
    TTGCTGGAGG CGGATATTAA ACTCTTCAGA AAATACTTTT GGGATAGAGC CTTTCTCATC
    AAGGCTCTTG AAGAGCAAGA AGGCACGTCA GAGGAGAAAC CAGATATGCC CTCCAGCGCT
    GCAGAAACTG AAGAAGGTAT GCATGCTCCT TCCCCACCTG GGGAACTCAG AAAGTGCTCA
    GCATTCTCTA GTCAATTGCG AGTTGAAGTG TGTGGAGAAG GAGAGCAGGA GCCGATGGAC
    TCGGAGGCGA GAGTAGGTTG CTCTGGGGCC GCAGGGCCCA GAGTTCCTTC AGCAGCACTG
    ACCAAAGAGA TTAAGAATTC GGGGTGCAGC AGATCACCAC CCGGGAAGGA GGGTGTAAAG
    CGTGTGAAAA GCCTGGTGGT TCCACTGAAT GAACATGAGG ACCAGGAGAG GCTAGATTTG
    GATCGTTCCT TTGATGCCCT CAAAAAATCC AAGCACCCCT CAACATTGCT TGCTGCTGGC
    CAGCTCTCTG ACATGCTGAA TGGTGGTGAT GACGTCTACG CTAACTGCAT GGTGATAGAC
    CAGGTTGGTG ATTTGGACAT TAACTATATT AATATAGAGG AAATCCCTGC AAATACCTGT
    CTTGAATCCA TGGATTCTTC TCTGGGGCCT GAGAGCAGCA AACCCAGCCT TCCTACTGAA
    GCCAGTGCTG GCACATACCC ACCAAATAAG AGGCCAGAAG TGACGTCACA CACGGAAGCC
    CAATCATCTT TCATATCATC TAGTTCGTAT GCTTCCAATT TGAATCTTTC TTTTGGTCTT
    CGTGGATTTG AAAAGGAGCA AAGTCGTCTA AAGAAAAGAA GTTCCAGCCT TGATGCCTTG
    GATGCTGACA GTGAAGGAGA GGGCCATTCG GAGCAGTCGC ACAATTGCTA CACTCCCGTG
    TGTCAGAGTT CCTCGAGAAC TGGCATTGCT AGCGGGGATG AATTGGACTC TTTTGAGACC
    AACACTGAAC CAGATTTTAA TATCTCCAGG ACGGAATCAT TTTCTTTATC AAGTAGCCTG
    CAGCTGAAGG AATCATTGTT TTCTGGAATC CGTTCACGTT CGTATTCCTG CTCATCACCC
    AAAATTTCTT TAGGAAAAAC TCGATTGATG CGTGATTTTA CAGTGTGTAA TTCAATTGAA
    GAGCAAAGAG CTTACAGCTT GCCGGAGCCA CCGAGAGAAC AAAGAATTCA GGAAGAAGAA
    TGGGATAAAT ATATCATACC TGCCAAATCA GAATCTGAAA AATACAAAGT AAGCCGAACT
    TTTAGCTTCC TCATGAATAG GATGACCAGC CCTCGGAATA AATCAAAGAC AAAAGGCAAG
    GATGCCAAAG ACAAAGAGAA ACTGACTCGA CATCAGTTTG TCCCTGGAAC TTTCTCTGGG
    GTTCTGCAGT GTTTGGTTTG CGATAAAACG CTCCTGGGGA AAGAGTCATT CCAGTGCTCC
    AATTCTTCGT TTCGAGACGT CCCATTGCCT GGGCTCTCTC TGCACCCTTC CTCCTCCATG
    CCTATCGGGT TGCCAACTGG GAGGAAGGAG ATGGCAGGGC AAGTGCATCC CTTGTCCAGA
    AGTGTCCCAG GCACCACCTT GGAAAGCTTC AGGAGGCCGG GGGCATCCTT GGAGTCCGAG
    AATGATGGCA GCACCTGGAG AAGCAGGTCT CACTCTGACG AGCTGTTCCA GCCCATGGGC
    TCGTCCCCCT CCACGGACTC CTTCTTAACG GAAGATGTCG TGGATTCTTC TCTGTGGAGT
    GACCTCAGCG GCGATGCCCA GGAGTTTGAA GCGGAATCTT GGAGTCTGGT GGTGGACCCT
    TCATTTTGTA GTCGGCAGGA GAAGGATGTC ATCCAAAGAC AGGATGTCAT TTTTGAGCTA
    ATGCAGACAG AACTGCATCA CATCCAGACC CTGTTCATCA TGTCTGAGAT CTTCAGGAAA
    GGGATGAAAG AGGAGCTGCA GCTGGACCAC AGCACTGTGG ACAAAATCTT CCCCTGTCTG
    GACGAGTTAC TTGAAATCCA CAAGCATTTC TTCTGTAGTA TGAAGGAGCG AAGGCAGGAA
    TCCTGTGTTG GCAATGACAG GAATTTCATC ATCGGCCGAA TTGGAGATAT TCTAGTACAA
    CAGTTTTCAG AAGAAAATGC AAATAAAATG AAGAAAATAT ATGGGGAATT CTGTAGCCAT
    CACAAAGAGG CTGTTAGCCT CTTTAAAGAA CTCCAGCAGA ATAAAAAGTT TCAGAATTTT
    ATTAAGCTCC GAAATAGTAA CCTTCTGGCT CGACGCCGAG GAATTCCAGA ATGCATTCTG
    CTGGTCACTC AGCGCATCAC AAAATACCCT GTCTTGGTGG AAAGGATATT GCAGTACACA
    AAGGAGAGCA CTGAAGAACA GAGAGACTTA TGCAAAGCTC TTTGCTTAAT TAAAGACATG
    ATTGCAGCTG TGGATTTAAA AGTCAGCGAA TATGAGAAAA AACAAAAATG GCTTGAGATC
    CTAAATAAGA TCGAAAACAA AACATACACC AAGCTCAAAA ATGGCCATGT GTTTAGGAAA
    CAGGCACTGA TGAGTAAAGA GAGGACCCTG TTATATGATG GCCTTGTCTA TTGGAAAACT
    GCTACAGGTC GTTTCAAAGA TATCCTAGCT CTACTTCTAA CTGATGTGCT GCTCTTTTTA
    CAAGAAAAAG ACCAGAAATA CATCTTTGCA GCTGTTGATC AGAAGCCATC AGTTATTTCC
    CTTCAGAAGC TCATTGCTAG GGAAGTTGCG AATGAGGAGA GAGGAATGTT TTTGATCAGC
    GCTTCATCTG CTGGTCCCGA GATGTATGAA ATCCATACCA ACTCCAAGGA GGAAAGAAAT
    ACCTGGATGA GACGGATCCA ACAGGCAGTA GAAAGTTGCC CAGAAGAAGA AGGGGGAAGG
    ACAAGTGAGT CTGATGAAGA GAGGAGGAAA GCTGAAGCCA GAGTGGCGAA GATTCAGCAA
    TGCCAAGAAA TACTCAGTAA CCAAGATCAA CAAATTTGCA CATATTTGGA AGAGAAACTG
    CATATCTACG CGAAACTTGG AGAACTAAGT GGATTTCAGG ATGTCCATCT AGAGCCCCAC
    CTCCTCATTC AACCAGACCC CGGAGAGCCT CCCCAGGCAG CCTCATTACT GGCCGCAGCA
    CTGAAAGAGG CTGAGAGTCT GCAGGTGGCA GTGAAGGCTT CCCAGATGAG CGATGCATGC
    CAGTCCCCCG AGGAAGGTTG TGGAGAACCT GCTTTGCCGG ACGTGCTCCA TTCTCAGGAC
    GCACCAGGAT CGTCTACTGC TTCACTAGTC ACAGAAGGGA CAGGAGGAAG AGGCTGTTGG
    GATGTGGATG CCGGGACCCA GGGTGTGGGA ACCGACCTGG CGGTTTCTGA TGCGGGGGAG
    AAGGTGGAAT ATAGAAGTTT CCCAGGTTCT TTGCAGTCAG AGATTATACA AGCGATACAG
    AATTTAACTC GTCTCTTATA TAGCCTTCAG GCCGCCTTGA CCATTCAGGA TAGCCACATT
    GAGATCCACA GGCTGGTTCT CCAACAGCGG GAGAGCCTGG CCCCGGGCCC CGCGTTCCGA
    GGCAGCCCCT TTCAGGACCA GGAGAAGTCC CGCAGCCTGG AGAAGCAGCG GGAGGAGCTG
    GCCGATACCC ACAAGCTGCA GCTCCAGTTC CAGCAAGACC AGCACCGCTG GCTCCGCAGG
    TGCGACCAGC AGCAGCGCGA GCAGGAGGCC CGGGCCAGCC GGCTGCAGGA GCGCGAGAGG
    GAGTGCCGGG CGCAGGAGGA GCTGCTGCTC AGGAGCCGGG GCGAGCTGGA CCAGCAGCTC
    CAAGACTACC AGCAGAGCCT GGAGCGCCTG CGCGAGGGCC AGCGCCTGGT GGAGAGGGAG
    CGGGAGAGGA TGCGGGCCCA GCAGAGCCTG CTGCGCCACT GGAAGCACGG GCGGCAGAGC
    AGCCTTCCCG CCGCGTTTTC TCCAGGAGCC CTGGAGGTAA TGGAACTTAA TCGATCTGAG
    ACTTTACGTC ATGAAAATTC ATTCTTCATC AATGAAGCTT TAGTGCAAAT GTCACTCAAC
    CCTTTGAACA AACCGCCGCC ACCAGGTATC CATCAGGATG CGGCTTACCC CCCAGGCATA
    CCTCATTCTG ATTTGGTAAG GACTACTGAA AATCAAGTAG ACCTCAAGAC GGACGTTTCT
    CAACCCTCAG ATATCAACCA TGAACTGTGG ATAGCGGCTG GGGCCTGTCA TCAGATACCC
    CCTCTCCACC AAAGCAGCAA GGATTCTTGT AAAAATGATG TGAACATCTC TCAGACTGCA
    TACCCACCCC TCCATGACTC AAATCCACAC AGCCCTCGGC TGCAGACGTT TATAGCAGAA
    GAAAAGCTAA ATCTACAGAG AGTGACCAGA CAAGACGGGG AAACTGGAAA TGGAGCAGAA
    GAAAATATTG TTTACCTCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa228082
    Clone ID Related Accession (Same CDS sequence) XM_024981396.1
    Accession Version XM_024981396.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5292bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product rho guanine nucleotide exchange factor 28 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGCAGCCCA CTCTTCACTT TCTTCCCATC TTTAGGTGTC CGTTCAGCTG TGAGCTCTGT 
    GAGGAGTGGA TCGTTTCCTG GTCATCTTTG TGCAATGCTG AGCCCATTGA AGATGCGGAA
    ACCATGGAGC TGAACTGCAG CGAAGTACCT CTGTATGGGC AGATGACGGT CTGCGCGAAG
    TTTGGCAAAA ATGTTTATCT TCCTAAAGAT GCTGAGTTTT ACTTTATTTA TAATGGATCT
    CATCAGAGAC ACATCGTGAT TGCAGAGCGC ACAGAAGATA ATGTTCTCCA GTCCAGCATT
    CCAGGCCACG GGCTTCAGGA GACGGTCACC GTGTCTGTGT GCCTGTGCTC GGAAGGTTAC
    TCCCCGGTGA CCATGGGCTC TGACTCCGTG ACCTACGTGG ACAACATGGC CTGCAGGCTG
    GCCCGTCTGC TGGTCACCCA GGCCGATCGC CTCACCGCCA GCAGCCACCA GACCTTGCTG
    ACCCCGTTTG CCCTGACGGC GGGGGCATTG CCTGCTCTGG ACGAGGAGCT CGTGCTGGCC
    CTGACTCATC TGGAGTTGCC TCTGGGGTGG ACCGTCTTGG GGAATTCTTC ACTGGAAGTT
    TCTCTGCATA GGGAGTCTCT TCTGCACCTG GCTATGAGAT GGGGCCTTGC TAAGCTTTCC
    CAGCTCCTTC TGTGTCTCCC TGGGGGAGCC CAGGCCCTGA CTTTACCCAA CGAGGAGGGT
    GCCACGCCAT TAGACTTGGC TTTACATGGT GGACATTCCA AGCTGGTTGA AGACATCACA
    AACTTCCAGG GCAGGCGTTC CCCAGGTTTC TCCTGCGTGC AGCTCAGCGA AGATGCCTCT
    CTGCGGTATA TTCACTCATC AGAAACTCTG ACCCTGACGC TTAACCACAC AGCCGAGCAT
    TTGCTGGAGG CGGATATTAA ACTCTTCAGA AAATACTTTT GGGATAGAGC CTTTCTCATC
    AAGGCTCTTG AAGAGCAAGA AGGCACGTCA GAGGAGAAAC CAGATATGCC CTCCAGCGCT
    GCAGAAACTG AAGAAGGTAT GCATGCTCCT TCCCCACCTG GGGAACTCAG AAAGTGCTCA
    GCATTCTCTA GTCAATTGCG AGTTGAAGTG TGTGGAGAAG GAGAGCAGGA GCCGATGGAC
    TCGGAGGCGA GAGTAGGTTG CTCTGGGGCC GCAGGGCCCA GAGTTCCTTC AGCAGCACTG
    ACCAAAGATC GTTCCTTTGA TGCCCTCAAA AAATCCAAGC ACCCCTCAAC ATTGCTTGCT
    GCTGGCCAGC TCTCTGACAT GCTGAATGGT GGTGATGACG TCTACGCTAA CTGCATGGTG
    ATAGACCAGG TTGGTGATTT GGACATTAAC TATATTAATA TAGAGGAAAT CCCTGCAAAT
    ACCTGTCTTG AATCCATGGA TTCTTCTCTG GGGCCTGAGA GCAGCAAACC CAGCCTTCCT
    ACTGAAGCCA GTGCTGGCAC ATACCCACCA AATAAGAGGC CAGAAGTGAC GTCACACACG
    GAAGCCCAAT CATCTTTCAT ATCATCTAGT TCGTATGCTT CCAATTTGAA TCTTTCTTTT
    GGTCTTCGTG GATTTGAAAA GGAGCAAAGT CGTCTAAAGA AAAGAAGTTC CAGCCTTGAT
    GCCTTGGATG CTGACAGTGA AGGAGAGGGC CATTCGGAGC AGTCGCACAA TTGCTACACT
    CCCGTGTGTC AGAGTTCCTC GAGAACTGGC ATTGCTAGCG GGGATGAATT GGACTCTTTT
    GAGACCAACA CTGAACCAGA TTTTAATATC TCCAGGACGG AATCATTTTC TTTATCAAGT
    AGCCTGCAGC TGAAGGAATC ATTGTTTTCT GGAATCCGTT CACGTTCGTA TTCCTGCTCA
    TCACCCAAAA TTTCTTTAGG AAAAACTCGA TTGATGCGTG ATTTTACAGT GTGTAATTCA
    ATTGAAGAGC AAAGAGCTTA CAGCTTGCCG GAGCCACCGA GAGAACAAAG AATTCAGGAA
    GAAGAATGGG ATAAATATAT CATACCTGCC AAATCAGAAT CTGAAAAATA CAAAGTAAGC
    CGAACTTTTA GCTTCCTCAT GAATAGGATG ACCAGCCCTC GGAATAAATC AAAGACAAAA
    GGCAAGGATG CCAAAGACAA AGAGAAACTG ACTCGACATC AGTTTGTCCC TGGAACTTTC
    TCTGGGGTTC TGCAGTGTTT GGTTTGCGAT AAAACGCTCC TGGGGAAAGA GTCATTCCAG
    TGCTCCAACT GTAATGGAAA CGTACATAAA GTCTGTAAAG ATGCTGCCCC TCCGTGTACC
    AAGAAATTCC AAGACAAATA TAATAAGAAC AAACCACAGA CCATCCTTGG AAATTCTTCG
    TTTCGAGACG TCCCATTGCC TGGGCTCTCT CTGCACCCTT CCTCCTCCAT GCCTATCGGG
    TTGCCAACTG GGAGGAAGGA GATGGCAGGG CAAGTGCATC CCTTGTCCAG AAGTGTCCCA
    GGCACCACCT TGGAAAGCTT CAGGAGGCCG GGGGCATCCT TGGAGTCCGA GAATGATGGC
    AGCACCTGGA GAAGCAGGTC TCACTCTGAC GAGCTGTTCC AGCCCATGGG CTCGTCCCCC
    TCCACGGACT CCTTCTTAAC GGAAGATGTC GTGGATTCTT CTCTGTGGAG TGACCTCAGC
    GGCGATGCCC AGGAGTTTGA AGCGGAATCT TGGAGTCTGG TGGTGGACCC TTCATTTTGT
    AGTCGGCAGG AGAAGGATGT CATCCAAAGA CAGGATGTCA TTTTTGAGCT AATGCAGACA
    GAACTGCATC ACATCCAGAC CCTGTTCATC ATGTCTGAGA TCTTCAGGAA AGGGATGAAA
    GAGGAGCTGC AGCTGGACCA CAGCACTGTG GACAAAATCT TCCCCTGTCT GGACGAGTTA
    CTTGAAATCC ACAAGCATTT CTTCTGTAGT ATGAAGGAGC GAAGGCAGGA ATCCTGTGTT
    GGCAATGACA GGAATTTCAT CATCGGCCGA ATTGGAGATA TTCTAGTACA ACAGTTTTCA
    GAAGAAAATG CAAATAAAAT GAAGAAAATA TATGGGGAAT TCTGTAGCCA TCACAAAGAG
    GCTGTTAGCC TCTTTAAAGA ACTCCAGCAG AATAAAAAGT TTCAGAATTT TATTAAGCTC
    CGAAATAGTA ACCTTCTGGC TCGACGCCGA GGAATTCCAG AATGCATTCT GCTGGTCACT
    CAGCGCATCA CAAAATACCC TGTCTTGGTG GAAAGGATAT TGCAGTACAC AAAGGAGAGC
    ACTGAAGAAC AGAGAGACTT ATGCAAAGCT CTTTGCTTAA TTAAAGACAT GATTGCAGCT
    GTGGATTTAA AAGTCAGCGA ATATGAGAAA AAACAAAAAT GGCTTGAGAT CCTAAATAAG
    ATCGAAAACA AAACATACAC CAAGCTCAAA AATGGCCATG TGTTTAGGAA ACAGGCACTG
    ATGAGTAAAG AGAGGACCCT GTTATATGAT GGCCTTGTCT ATTGGAAAAC TGCTACAGGT
    CGTTTCAAAG ATATCCTAGC TCTACTTCTA ACTGATGTGC TGCTCTTTTT ACAAGAAAAA
    GACCAGAAAT ACATCTTTGC AGCTGTTGAT CAGAAGCCAT CAGTTATTTC CCTTCAGAAG
    CTCATTGCTA GGGAAGTTGC GAATGAGGAG AGAGGAATGT TTTTGATCAG CGCTTCATCT
    GCTGGTCCCG AGATGTATGA AATCCATACC AACTCCAAGG AGGAAAGAAA TACCTGGATG
    AGACGGATCC AACAGGCAGT AGAAAGTTGC CCAGAAGAAG AAGGGGGAAG GACAAGTGAG
    TCTGATGAAG AGAGGAGGAA AGCTGAAGCC AGAGTGGCGA AGATTCAGCA ATGCCAAGAA
    ATACTCAGTA ACCAAGATCA ACAAATTTGC ACATATTTGG AAGAGAAACT GCATATCTAC
    GCGAAACTTG GAGAACTAAG TGGATTTCAG GATGTCCATC TAGAGCCCCA CCTCCTCATT
    CAACCAGACC CCGGAGAGCC TCCCCAGGCA GCCTCATTAC TGGCCGCAGC ACTGAAAGAG
    GCTGAGAGTC TGCAGGTGGC AGTGAAGGCT TCCCAGATGA GCGATGCATG CCAGTCCCCC
    GAGGAAGGTT GTGGAGAACC TGCTTTGCCG GACGTGCTCC ATTCTCAGGA CGCACCAGGA
    TCGTCTACTG CTTCACTAGT CACAGAAGGG ACAGGAGGAA GAGGCTGTTG GGATGTGGAT
    GCCGGGACCC AGGGTGTGGG AACCGACCTG GCGGTTTCTG ATGCGGGGGA GAAGGTGGAA
    TATAGAAGTT TCCCAGGTTC TTTGCAGTCA GAGATTATAC AAGCGATACA GAATTTAACT
    CGTCTCTTAT ATAGCCTTCA GGCCGCCTTG ACCATTCAGG ATAGCCACAT TGAGATCCAC
    AGGCTGGTTC TCCAACAGCG GGAGAGCCTG GCCCCGGGCC CCGCGTTCCG AGGCAGCCCC
    TTTCAGGACC AGGAGAAGTC CCGCAGCCTG GAGAAGCAGC GGGAGGAGCT GGCCGATACC
    CACAAGCTGC AGCTCCAGTT CCAGCAAGAC CAGCACCGCT GGCTCCGCAG GTGCGACCAG
    CAGCAGCGCG AGCAGGAGGC CCGGGCCAGC CGGCTGCAGG AGCGCGAGAG GGAGTGCCGG
    GCGCAGGAGG AGCTGCTGCT CAGGAGCCGG GGCGAGCTGG ACCAGCAGCT CCAAGACTAC
    CAGCAGAGCC TGGAGCGCCT GCGCGAGGGC CAGCGCCTGG TGGAGAGGGA GCGGGAGAGG
    ATGCGGGCCC AGCAGAGCCT GCTGCGCCAC TGGAAGCACG GGCGGCAGAG CAGCCTTCCC
    GCCGCGTTTT CTCCAGGAGC CCTGGAGGTA ATGGAACTTA ATCGATCTGA GACTTTACGT
    CATGAAAATT CATTCTTCAT CAATGAAGCT TTAGTGCAAA TGTCACTCAA CCCTTTGAAC
    AAACCGCCGC CACCAGGTAT CCATCAGGAT GCGGCTTACC CCCCAGGCAT ACCTCATTCT
    GATTTGGTAA GGACTACTGA AAATCAAGTA GACCTCAAGA CGGACGTTTC TCAACCCTCA
    GATATCAACC ATGAACTGTG GATAGCGGCT GGGGCCTGTC ATCAGATACC CCCTCTCCAC
    CAAAGCAGCA AGGATTCTTG TAAAAATGAT GTGAACATCT CTCAGACTGC ATACCCACCC
    CTCCATGACT CAAATCCACA CAGCCCTCGG CTGCAGACGT TTATAGCAGA AGAAAAGCTA
    AATCTACAGA GAGTGACCAG ACAAGACGGG GAAACTGGAA ATGGAGCAGA AGAAAATATT
    GTTTACCTCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024837164.1
    CDS19..5310
    Translation

    Target ORF information:

    RefSeq Version XM_024981396.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981396.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGCAGCCCA CTCTTCACTT TCTTCCCATC TTTAGGTGTC CGTTCAGCTG TGAGCTCTGT 
    GAGGAGTGGA TCGTTTCCTG GTCATCTTTG TGCAATGCTG AGCCCATTGA AGATGCGGAA
    ACCATGGAGC TGAACTGCAG CGAAGTACCT CTGTATGGGC AGATGACGGT CTGCGCGAAG
    TTTGGCAAAA ATGTTTATCT TCCTAAAGAT GCTGAGTTTT ACTTTATTTA TAATGGATCT
    CATCAGAGAC ACATCGTGAT TGCAGAGCGC ACAGAAGATA ATGTTCTCCA GTCCAGCATT
    CCAGGCCACG GGCTTCAGGA GACGGTCACC GTGTCTGTGT GCCTGTGCTC GGAAGGTTAC
    TCCCCGGTGA CCATGGGCTC TGACTCCGTG ACCTACGTGG ACAACATGGC CTGCAGGCTG
    GCCCGTCTGC TGGTCACCCA GGCCGATCGC CTCACCGCCA GCAGCCACCA GACCTTGCTG
    ACCCCGTTTG CCCTGACGGC GGGGGCATTG CCTGCTCTGG ACGAGGAGCT CGTGCTGGCC
    CTGACTCATC TGGAGTTGCC TCTGGGGTGG ACCGTCTTGG GGAATTCTTC ACTGGAAGTT
    TCTCTGCATA GGGAGTCTCT TCTGCACCTG GCTATGAGAT GGGGCCTTGC TAAGCTTTCC
    CAGCTCCTTC TGTGTCTCCC TGGGGGAGCC CAGGCCCTGA CTTTACCCAA CGAGGAGGGT
    GCCACGCCAT TAGACTTGGC TTTACATGGT GGACATTCCA AGCTGGTTGA AGACATCACA
    AACTTCCAGG GCAGGCGTTC CCCAGGTTTC TCCTGCGTGC AGCTCAGCGA AGATGCCTCT
    CTGCGGTATA TTCACTCATC AGAAACTCTG ACCCTGACGC TTAACCACAC AGCCGAGCAT
    TTGCTGGAGG CGGATATTAA ACTCTTCAGA AAATACTTTT GGGATAGAGC CTTTCTCATC
    AAGGCTCTTG AAGAGCAAGA AGGCACGTCA GAGGAGAAAC CAGATATGCC CTCCAGCGCT
    GCAGAAACTG AAGAAGGTAT GCATGCTCCT TCCCCACCTG GGGAACTCAG AAAGTGCTCA
    GCATTCTCTA GTCAATTGCG AGTTGAAGTG TGTGGAGAAG GAGAGCAGGA GCCGATGGAC
    TCGGAGGCGA GAGTAGGTTG CTCTGGGGCC GCAGGGCCCA GAGTTCCTTC AGCAGCACTG
    ACCAAAGATC GTTCCTTTGA TGCCCTCAAA AAATCCAAGC ACCCCTCAAC ATTGCTTGCT
    GCTGGCCAGC TCTCTGACAT GCTGAATGGT GGTGATGACG TCTACGCTAA CTGCATGGTG
    ATAGACCAGG TTGGTGATTT GGACATTAAC TATATTAATA TAGAGGAAAT CCCTGCAAAT
    ACCTGTCTTG AATCCATGGA TTCTTCTCTG GGGCCTGAGA GCAGCAAACC CAGCCTTCCT
    ACTGAAGCCA GTGCTGGCAC ATACCCACCA AATAAGAGGC CAGAAGTGAC GTCACACACG
    GAAGCCCAAT CATCTTTCAT ATCATCTAGT TCGTATGCTT CCAATTTGAA TCTTTCTTTT
    GGTCTTCGTG GATTTGAAAA GGAGCAAAGT CGTCTAAAGA AAAGAAGTTC CAGCCTTGAT
    GCCTTGGATG CTGACAGTGA AGGAGAGGGC CATTCGGAGC AGTCGCACAA TTGCTACACT
    CCCGTGTGTC AGAGTTCCTC GAGAACTGGC ATTGCTAGCG GGGATGAATT GGACTCTTTT
    GAGACCAACA CTGAACCAGA TTTTAATATC TCCAGGACGG AATCATTTTC TTTATCAAGT
    AGCCTGCAGC TGAAGGAATC ATTGTTTTCT GGAATCCGTT CACGTTCGTA TTCCTGCTCA
    TCACCCAAAA TTTCTTTAGG AAAAACTCGA TTGATGCGTG ATTTTACAGT GTGTAATTCA
    ATTGAAGAGC AAAGAGCTTA CAGCTTGCCG GAGCCACCGA GAGAACAAAG AATTCAGGAA
    GAAGAATGGG ATAAATATAT CATACCTGCC AAATCAGAAT CTGAAAAATA CAAAGTAAGC
    CGAACTTTTA GCTTCCTCAT GAATAGGATG ACCAGCCCTC GGAATAAATC AAAGACAAAA
    GGCAAGGATG CCAAAGACAA AGAGAAACTG ACTCGACATC AGTTTGTCCC TGGAACTTTC
    TCTGGGGTTC TGCAGTGTTT GGTTTGCGAT AAAACGCTCC TGGGGAAAGA GTCATTCCAG
    TGCTCCAACT GTAATGGAAA CGTACATAAA GTCTGTAAAG ATGCTGCCCC TCCGTGTACC
    AAGAAATTCC AAGACAAATA TAATAAGAAC AAACCACAGA CCATCCTTGG AAATTCTTCG
    TTTCGAGACG TCCCATTGCC TGGGCTCTCT CTGCACCCTT CCTCCTCCAT GCCTATCGGG
    TTGCCAACTG GGAGGAAGGA GATGGCAGGG CAAGTGCATC CCTTGTCCAG AAGTGTCCCA
    GGCACCACCT TGGAAAGCTT CAGGAGGCCG GGGGCATCCT TGGAGTCCGA GAATGATGGC
    AGCACCTGGA GAAGCAGGTC TCACTCTGAC GAGCTGTTCC AGCCCATGGG CTCGTCCCCC
    TCCACGGACT CCTTCTTAAC GGAAGATGTC GTGGATTCTT CTCTGTGGAG TGACCTCAGC
    GGCGATGCCC AGGAGTTTGA AGCGGAATCT TGGAGTCTGG TGGTGGACCC TTCATTTTGT
    AGTCGGCAGG AGAAGGATGT CATCCAAAGA CAGGATGTCA TTTTTGAGCT AATGCAGACA
    GAACTGCATC ACATCCAGAC CCTGTTCATC ATGTCTGAGA TCTTCAGGAA AGGGATGAAA
    GAGGAGCTGC AGCTGGACCA CAGCACTGTG GACAAAATCT TCCCCTGTCT GGACGAGTTA
    CTTGAAATCC ACAAGCATTT CTTCTGTAGT ATGAAGGAGC GAAGGCAGGA ATCCTGTGTT
    GGCAATGACA GGAATTTCAT CATCGGCCGA ATTGGAGATA TTCTAGTACA ACAGTTTTCA
    GAAGAAAATG CAAATAAAAT GAAGAAAATA TATGGGGAAT TCTGTAGCCA TCACAAAGAG
    GCTGTTAGCC TCTTTAAAGA ACTCCAGCAG AATAAAAAGT TTCAGAATTT TATTAAGCTC
    CGAAATAGTA ACCTTCTGGC TCGACGCCGA GGAATTCCAG AATGCATTCT GCTGGTCACT
    CAGCGCATCA CAAAATACCC TGTCTTGGTG GAAAGGATAT TGCAGTACAC AAAGGAGAGC
    ACTGAAGAAC AGAGAGACTT ATGCAAAGCT CTTTGCTTAA TTAAAGACAT GATTGCAGCT
    GTGGATTTAA AAGTCAGCGA ATATGAGAAA AAACAAAAAT GGCTTGAGAT CCTAAATAAG
    ATCGAAAACA AAACATACAC CAAGCTCAAA AATGGCCATG TGTTTAGGAA ACAGGCACTG
    ATGAGTAAAG AGAGGACCCT GTTATATGAT GGCCTTGTCT ATTGGAAAAC TGCTACAGGT
    CGTTTCAAAG ATATCCTAGC TCTACTTCTA ACTGATGTGC TGCTCTTTTT ACAAGAAAAA
    GACCAGAAAT ACATCTTTGC AGCTGTTGAT CAGAAGCCAT CAGTTATTTC CCTTCAGAAG
    CTCATTGCTA GGGAAGTTGC GAATGAGGAG AGAGGAATGT TTTTGATCAG CGCTTCATCT
    GCTGGTCCCG AGATGTATGA AATCCATACC AACTCCAAGG AGGAAAGAAA TACCTGGATG
    AGACGGATCC AACAGGCAGT AGAAAGTTGC CCAGAAGAAG AAGGGGGAAG GACAAGTGAG
    TCTGATGAAG AGAGGAGGAA AGCTGAAGCC AGAGTGGCGA AGATTCAGCA ATGCCAAGAA
    ATACTCAGTA ACCAAGATCA ACAAATTTGC ACATATTTGG AAGAGAAACT GCATATCTAC
    GCGAAACTTG GAGAACTAAG TGGATTTCAG GATGTCCATC TAGAGCCCCA CCTCCTCATT
    CAACCAGACC CCGGAGAGCC TCCCCAGGCA GCCTCATTAC TGGCCGCAGC ACTGAAAGAG
    GCTGAGAGTC TGCAGGTGGC AGTGAAGGCT TCCCAGATGA GCGATGCATG CCAGTCCCCC
    GAGGAAGGTT GTGGAGAACC TGCTTTGCCG GACGTGCTCC ATTCTCAGGA CGCACCAGGA
    TCGTCTACTG CTTCACTAGT CACAGAAGGG ACAGGAGGAA GAGGCTGTTG GGATGTGGAT
    GCCGGGACCC AGGGTGTGGG AACCGACCTG GCGGTTTCTG ATGCGGGGGA GAAGGTGGAA
    TATAGAAGTT TCCCAGGTTC TTTGCAGTCA GAGATTATAC AAGCGATACA GAATTTAACT
    CGTCTCTTAT ATAGCCTTCA GGCCGCCTTG ACCATTCAGG ATAGCCACAT TGAGATCCAC
    AGGCTGGTTC TCCAACAGCG GGAGAGCCTG GCCCCGGGCC CCGCGTTCCG AGGCAGCCCC
    TTTCAGGACC AGGAGAAGTC CCGCAGCCTG GAGAAGCAGC GGGAGGAGCT GGCCGATACC
    CACAAGCTGC AGCTCCAGTT CCAGCAAGAC CAGCACCGCT GGCTCCGCAG GTGCGACCAG
    CAGCAGCGCG AGCAGGAGGC CCGGGCCAGC CGGCTGCAGG AGCGCGAGAG GGAGTGCCGG
    GCGCAGGAGG AGCTGCTGCT CAGGAGCCGG GGCGAGCTGG ACCAGCAGCT CCAAGACTAC
    CAGCAGAGCC TGGAGCGCCT GCGCGAGGGC CAGCGCCTGG TGGAGAGGGA GCGGGAGAGG
    ATGCGGGCCC AGCAGAGCCT GCTGCGCCAC TGGAAGCACG GGCGGCAGAG CAGCCTTCCC
    GCCGCGTTTT CTCCAGGAGC CCTGGAGGTA ATGGAACTTA ATCGATCTGA GACTTTACGT
    CATGAAAATT CATTCTTCAT CAATGAAGCT TTAGTGCAAA TGTCACTCAA CCCTTTGAAC
    AAACCGCCGC CACCAGGTAT CCATCAGGAT GCGGCTTACC CCCCAGGCAT ACCTCATTCT
    GATTTGGTAA GGACTACTGA AAATCAAGTA GACCTCAAGA CGGACGTTTC TCAACCCTCA
    GATATCAACC ATGAACTGTG GATAGCGGCT GGGGCCTGTC ATCAGATACC CCCTCTCCAC
    CAAAGCAGCA AGGATTCTTG TAAAAATGAT GTGAACATCT CTCAGACTGC ATACCCACCC
    CTCCATGACT CAAATCCACA CAGCCCTCGG CTGCAGACGT TTATAGCAGA AGAAAAGCTA
    AATCTACAGA GAGTGACCAG ACAAGACGGG GAAACTGGAA ATGGAGCAGA AGAAAATATT
    GTTTACCTCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa228083
    Clone ID Related Accession (Same CDS sequence) XM_024981397.1 , XM_024981398.1 , XM_024981399.1
    Accession Version XM_024981397.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5283bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product rho guanine nucleotide exchange factor 28 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGAGCTGA ACTGCAGCGA AGTACCTCTG TATGGGCAGA TGACGGTCTG CGCGAAGTTT 
    GGCAAAAATG TTTATCTTCC TAAAGATGCT GAGTTTTACT TTATTTATAA TGGATCTCAT
    CAGAGACACA TCGTGATTGC AGAGCGCACA GAAGATAATG TTCTCCAGTC CAGCATTCCA
    GGCCACGGGC TTCAGGAGAC GGTCACCGTG TCTGTGTGCC TGTGCTCGGA AGGTTACTCC
    CCGGTGACCA TGGGCTCTGA CTCCGTGACC TACGTGGACA ACATGGCCTG CAGGCTGGCC
    CGTCTGCTGG TCACCCAGGC CGATCGCCTC ACCGCCAGCA GCCACCAGAC CTTGCTGACC
    CCGTTTGCCC TGACGGCGGG GGCATTGCCT GCTCTGGACG AGGAGCTCGT GCTGGCCCTG
    ACTCATCTGG AGTTGCCTCT GGGGTGGACC GTCTTGGGGA ATTCTTCACT GGAAGTTTCT
    CTGCATAGGG AGTCTCTTCT GCACCTGGCT ATGAGATGGG GCCTTGCTAA GCTTTCCCAG
    CTCCTTCTGT GTCTCCCTGG GGGAGCCCAG GCCCTGACTT TACCCAACGA GGAGGGTGCC
    ACGCCATTAG ACTTGGCTTT ACATGGTGGA CATTCCAAGC TGGTTGAAGA CATCACAAAC
    TTCCAGGGCA GGCGTTCCCC AGGTTTCTCC TGCGTGCAGC TCAGCGAAGA TGCCTCTCTG
    CGGTATATTC ACTCATCAGA AACTCTGACC CTGACGCTTA ACCACACAGC CGAGCATTTG
    CTGGAGGCGG ATATTAAACT CTTCAGAAAA TACTTTTGGG ATAGAGCCTT TCTCATCAAG
    GCTCTTGAAG AGCAAGAAGG CACGTCAGAG GAGAAACCAG ATATGCCCTC CAGCGCTGCA
    GAAACTGAAG AAGGTATGCA TGCTCCTTCC CCACCTGGGG AACTCAGAAA GTGCTCAGCA
    TTCTCTAGTC AATTGCGAGT TGAAGTGTGT GGAGAAGGAG AGCAGGAGCC GATGGACTCG
    GAGGCGAGAG TAGGTTGCTC TGGGGCCGCA GGGCCCAGAG TTCCTTCAGC AGCACTGACC
    AAAGAGATTA AGAATTCGGG GTGCAGCAGA TCACCACCCG GGAAGGAGGG TGTAAAGCGT
    GTGAAAAGCC TGGTGGTTCC ACTGAATGAA CATGAGGACC AGGAGAGGCT AGATTTGGAT
    CGTTCCTTTG ATGCCCTCAA AAAATCCAAG CACCCCTCAA CATTGCTTGC TGCTGGCCAG
    CTCTCTGACA TGCTGAATGG TGGTGATGAC GTCTACGCTA ACTGCATGGT GATAGACCAG
    GTTGGTGATT TGGACATTAA CTATATTAAT ATAGAGGAAA TCCCTGCAAA TACCTGTCTT
    GAATCCATGG ATTCTTCTCT GGGGCCTGAG AGCAGCAAAC CCAGCCTTCC TACTGAAGCC
    AGTGCTGGCA CATACCCACC AAATAAGAGG CCAGAAGTGA CGTCACACAC GGAAGCCCAA
    TCATCTTTCA TATCATCTAG TTCGTATGCT TCCAATTTGA ATCTTTCTTT TGGTCTTCGT
    GGATTTGAAA AGGAGCAAAG TCGTCTAAAG AAAAGAAGTT CCAGCCTTGA TGCCTTGGAT
    GCTGACAGTG AAGGAGAGGG CCATTCGGAG CAGTCGCACA ATTGCTACAC TCCCGTGTGT
    CAGAGTTCCT CGAGAACTGG CATTGCTAGC GGGGATGAAT TGGACTCTTT TGAGACCAAC
    ACTGAACCAG ATTTTAATAT CTCCAGGACG GAATCATTTT CTTTATCAAG TAGCCTGCAG
    CTGAAGGAAT CATTGTTTTC TGGAATCCGT TCACGTTCGT ATTCCTGCTC ATCACCCAAA
    ATTTCTTTAG GAAAAACTCG ATTGATGCGT GATTTTACAG TGTGTAATTC AATTGAAGAG
    CAAAGAGCTT ACAGCTTGCC GGAGCCACCG AGAGAACAAA GAATTCAGGA AGAAGAATGG
    GATAAATATA TCATACCTGC CAAATCAGAA TCTGAAAAAT ACAAAGTAAG CCGAACTTTT
    AGCTTCCTCA TGAATAGGAT GACCAGCCCT CGGAATAAAT CAAAGACAAA AGGCAAGGAT
    GCCAAAGACA AAGAGAAACT GACTCGACAT CAGTTTGTCC CTGGAACTTT CTCTGGGGTT
    CTGCAGTGTT TGGTTTGCGA TAAAACGCTC CTGGGGAAAG AGTCATTCCA GTGCTCCAAC
    TGTAATGGAA ACGTACATAA AGTCTGTAAA GATGCTGCCC CTCCGTGTAC CAAGAAATTC
    CAAGACAAAT ATAATAAGAA CAAACCACAG ACCATCCTTG GAAATTCTTC GTTTCGAGAC
    GTCCCATTGC CTGGGCTCTC TCTGCACCCT TCCTCCTCCA TGCCTATCGG GTTGCCAACT
    GGGAGGAAGG AGATGGCAGG GCAAGTGCAT CCCTTGTCCA GAAGTGTCCC AGGCACCACC
    TTGGAAAGCT TCAGGAGGCC GGGGGCATCC TTGGAGTCCG AGAATGATGG CAGCACCTGG
    AGAAGCAGGT CTCACTCTGA CGAGCTGTTC CAGCCCATGG GCTCGTCCCC CTCCACGGAC
    TCCTTCTTAA CGGAAGATGT CGTGGATTCT TCTCTGTGGA GTGACCTCAG CGGCGATGCC
    CAGGAGTTTG AAGCGGAATC TTGGAGTCTG GTGGTGGACC CTTCATTTTG TAGTCGGCAG
    GAGAAGGATG TCATCCAAAG ACAGGATGTC ATTTTTGAGC TAATGCAGAC AGAACTGCAT
    CACATCCAGA CCCTGTTCAT CATGTCTGAG ATCTTCAGGA AAGGGATGAA AGAGGAGCTG
    CAGCTGGACC ACAGCACTGT GGACAAAATC TTCCCCTGTC TGGACGAGTT ACTTGAAATC
    CACAAGCATT TCTTCTGTAG TATGAAGGAG CGAAGGCAGG AATCCTGTGT TGGCAATGAC
    AGGAATTTCA TCATCGGCCG AATTGGAGAT ATTCTAGTAC AACAGTTTTC AGAAGAAAAT
    GCAAATAAAA TGAAGAAAAT ATATGGGGAA TTCTGTAGCC ATCACAAAGA GGCTGTTAGC
    CTCTTTAAAG AACTCCAGCA GAATAAAAAG TTTCAGAATT TTATTAAGCT CCGAAATAGT
    AACCTTCTGG CTCGACGCCG AGGAATTCCA GAATGCATTC TGCTGGTCAC TCAGCGCATC
    ACAAAATACC CTGTCTTGGT GGAAAGGATA TTGCAGTACA CAAAGGAGAG CACTGAAGAA
    CAGAGAGACT TATGCAAAGC TCTTTGCTTA ATTAAAGACA TGATTGCAGC TGTGGATTTA
    AAAGTCAGCG AATATGAGAA AAAACAAAAA TGGCTTGAGA TCCTAAATAA GATCGAAAAC
    AAAACATACA CCAAGCTCAA AAATGGCCAT GTGTTTAGGA AACAGGCACT GATGAGTAAA
    GAGAGGACCC TGTTATATGA TGGCCTTGTC TATTGGAAAA CTGCTACAGG TCGTTTCAAA
    GATATCCTAG CTCTACTTCT AACTGATGTG CTGCTCTTTT TACAAGAAAA AGACCAGAAA
    TACATCTTTG CAGCTGTTGA TCAGAAGCCA TCAGTTATTT CCCTTCAGAA GCTCATTGCT
    AGGGAAGTTG CGAATGAGGA GAGAGGAATG TTTTTGATCA GCGCTTCATC TGCTGGTCCC
    GAGATGTATG AAATCCATAC CAACTCCAAG GAGGAAAGAA ATACCTGGAT GAGACGGATC
    CAACAGGCAG TAGAAAGTTG CCCAGAAGAA GAAGGGGGAA GGACAAGTGA GTCTGATGAA
    GAGAGGAGGA AAGCTGAAGC CAGAGTGGCG AAGATTCAGC AATGCCAAGA AATACTCAGT
    AACCAAGATC AACAAATTTG CACATATTTG GAAGAGAAAC TGCATATCTA CGCGAAACTT
    GGAGAACTAA GTGGATTTCA GGATGTCCAT CTAGAGCCCC ACCTCCTCAT TCAACCAGAC
    CCCGGAGAGC CTCCCCAGGC AGCCTCATTA CTGGCCGCAG CACTGAAAGA GGCTGAGAGT
    CTGCAGGTGG CAGTGAAGGC TTCCCAGATG AGCGATGCAT GCCAGTCCCC CGAGGAAGGT
    TGTGGAGAAC CTGCTTTGCC GGACGTGCTC CATTCTCAGG ACGCACCAGG ATCGTCTACT
    GCTTCACTAG TCACAGAAGG GACAGGAGGA AGAGGCTGTT GGGATGTGGA TGCCGGGACC
    CAGGGTGTGG GAACCGACCT GGCGGTTTCT GATGCGGGGG AGAAGGTGGA ATATAGAAGT
    TTCCCAGGTT CTTTGCAGTC AGAGATTATA CAAGCGATAC AGAATTTAAC TCGTCTCTTA
    TATAGCCTTC AGGCCGCCTT GACCATTCAG GATAGCCACA TTGAGATCCA CAGGCTGGTT
    CTCCAACAGC GGGAGAGCCT GGCCCCGGGC CCCGCGTTCC GAGGCAGCCC CTTTCAGGAC
    CAGGAGAAGT CCCGCAGCCT GGAGAAGCAG CGGGAGGAGC TGGCCGATAC CCACAAGCTG
    CAGCTCCAGT TCCAGCAAGA CCAGCACCGC TGGCTCCGCA GGTGCGACCA GCAGCAGCGC
    GAGCAGGAGG CCCGGGCCAG CCGGCTGCAG GAGCGCGAGA GGGAGTGCCG GGCGCAGGAG
    GAGCTGCTGC TCAGGAGCCG GGGCGAGCTG GACCAGCAGC TCCAAGACTA CCAGCAGAGC
    CTGGAGCGCC TGCGCGAGGG CCAGCGCCTG GTGGAGAGGG AGCGGGAGAG GATGCGGGCC
    CAGCAGAGCC TGCTGCGCCA CTGGAAGCAC GGGCGGCAGA GCAGCCTTCC CGCCGCGTTT
    TCTCCAGGAG CCCTGGAGGT AATGGAACTT AATCGATCTG AGACTTTACG TCATGAAAAT
    TCATTCTTCA TCAATGAAGC TTTAGTGCAA ATGTCACTCA ACCCTTTGAA CAAACCGCCG
    CCACCAGGTA TCCATCAGGA TGCGGCTTAC CCCCCAGGCA TACCTCATTC TGATTTGGTA
    AGGACTACTG AAAATCAAGT AGACCTCAAG ACGGACGTTT CTCAACCCTC AGATATCAAC
    CATGAACTGT GGATAGCGGC TGGGGCCTGT CATCAGATAC CCCCTCTCCA CCAAAGCAGC
    AAGGATTCTT GTAAAAATGA TGTGAACATC TCTCAGACTG CATACCCACC CCTCCATGAC
    TCAAATCCAC ACAGCCCTCG GCTGCAGACG TTTATAGCAG AAGAAAAGCT AAATCTACAG
    AGAGTGACCA GACAAGACGG GGAAACTGGA AATGGAGCAG AAGAAAATAT TGTTTACCTC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024837165.1
    CDS138..5420
    Translation

    Target ORF information:

    RefSeq Version XM_024981397.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981397.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGAGCTGA ACTGCAGCGA AGTACCTCTG TATGGGCAGA TGACGGTCTG CGCGAAGTTT 
    GGCAAAAATG TTTATCTTCC TAAAGATGCT GAGTTTTACT TTATTTATAA TGGATCTCAT
    CAGAGACACA TCGTGATTGC AGAGCGCACA GAAGATAATG TTCTCCAGTC CAGCATTCCA
    GGCCACGGGC TTCAGGAGAC GGTCACCGTG TCTGTGTGCC TGTGCTCGGA AGGTTACTCC
    CCGGTGACCA TGGGCTCTGA CTCCGTGACC TACGTGGACA ACATGGCCTG CAGGCTGGCC
    CGTCTGCTGG TCACCCAGGC CGATCGCCTC ACCGCCAGCA GCCACCAGAC CTTGCTGACC
    CCGTTTGCCC TGACGGCGGG GGCATTGCCT GCTCTGGACG AGGAGCTCGT GCTGGCCCTG
    ACTCATCTGG AGTTGCCTCT GGGGTGGACC GTCTTGGGGA ATTCTTCACT GGAAGTTTCT
    CTGCATAGGG AGTCTCTTCT GCACCTGGCT ATGAGATGGG GCCTTGCTAA GCTTTCCCAG
    CTCCTTCTGT GTCTCCCTGG GGGAGCCCAG GCCCTGACTT TACCCAACGA GGAGGGTGCC
    ACGCCATTAG ACTTGGCTTT ACATGGTGGA CATTCCAAGC TGGTTGAAGA CATCACAAAC
    TTCCAGGGCA GGCGTTCCCC AGGTTTCTCC TGCGTGCAGC TCAGCGAAGA TGCCTCTCTG
    CGGTATATTC ACTCATCAGA AACTCTGACC CTGACGCTTA ACCACACAGC CGAGCATTTG
    CTGGAGGCGG ATATTAAACT CTTCAGAAAA TACTTTTGGG ATAGAGCCTT TCTCATCAAG
    GCTCTTGAAG AGCAAGAAGG CACGTCAGAG GAGAAACCAG ATATGCCCTC CAGCGCTGCA
    GAAACTGAAG AAGGTATGCA TGCTCCTTCC CCACCTGGGG AACTCAGAAA GTGCTCAGCA
    TTCTCTAGTC AATTGCGAGT TGAAGTGTGT GGAGAAGGAG AGCAGGAGCC GATGGACTCG
    GAGGCGAGAG TAGGTTGCTC TGGGGCCGCA GGGCCCAGAG TTCCTTCAGC AGCACTGACC
    AAAGAGATTA AGAATTCGGG GTGCAGCAGA TCACCACCCG GGAAGGAGGG TGTAAAGCGT
    GTGAAAAGCC TGGTGGTTCC ACTGAATGAA CATGAGGACC AGGAGAGGCT AGATTTGGAT
    CGTTCCTTTG ATGCCCTCAA AAAATCCAAG CACCCCTCAA CATTGCTTGC TGCTGGCCAG
    CTCTCTGACA TGCTGAATGG TGGTGATGAC GTCTACGCTA ACTGCATGGT GATAGACCAG
    GTTGGTGATT TGGACATTAA CTATATTAAT ATAGAGGAAA TCCCTGCAAA TACCTGTCTT
    GAATCCATGG ATTCTTCTCT GGGGCCTGAG AGCAGCAAAC CCAGCCTTCC TACTGAAGCC
    AGTGCTGGCA CATACCCACC AAATAAGAGG CCAGAAGTGA CGTCACACAC GGAAGCCCAA
    TCATCTTTCA TATCATCTAG TTCGTATGCT TCCAATTTGA ATCTTTCTTT TGGTCTTCGT
    GGATTTGAAA AGGAGCAAAG TCGTCTAAAG AAAAGAAGTT CCAGCCTTGA TGCCTTGGAT
    GCTGACAGTG AAGGAGAGGG CCATTCGGAG CAGTCGCACA ATTGCTACAC TCCCGTGTGT
    CAGAGTTCCT CGAGAACTGG CATTGCTAGC GGGGATGAAT TGGACTCTTT TGAGACCAAC
    ACTGAACCAG ATTTTAATAT CTCCAGGACG GAATCATTTT CTTTATCAAG TAGCCTGCAG
    CTGAAGGAAT CATTGTTTTC TGGAATCCGT TCACGTTCGT ATTCCTGCTC ATCACCCAAA
    ATTTCTTTAG GAAAAACTCG ATTGATGCGT GATTTTACAG TGTGTAATTC AATTGAAGAG
    CAAAGAGCTT ACAGCTTGCC GGAGCCACCG AGAGAACAAA GAATTCAGGA AGAAGAATGG
    GATAAATATA TCATACCTGC CAAATCAGAA TCTGAAAAAT ACAAAGTAAG CCGAACTTTT
    AGCTTCCTCA TGAATAGGAT GACCAGCCCT CGGAATAAAT CAAAGACAAA AGGCAAGGAT
    GCCAAAGACA AAGAGAAACT GACTCGACAT CAGTTTGTCC CTGGAACTTT CTCTGGGGTT
    CTGCAGTGTT TGGTTTGCGA TAAAACGCTC CTGGGGAAAG AGTCATTCCA GTGCTCCAAC
    TGTAATGGAA ACGTACATAA AGTCTGTAAA GATGCTGCCC CTCCGTGTAC CAAGAAATTC
    CAAGACAAAT ATAATAAGAA CAAACCACAG ACCATCCTTG GAAATTCTTC GTTTCGAGAC
    GTCCCATTGC CTGGGCTCTC TCTGCACCCT TCCTCCTCCA TGCCTATCGG GTTGCCAACT
    GGGAGGAAGG AGATGGCAGG GCAAGTGCAT CCCTTGTCCA GAAGTGTCCC AGGCACCACC
    TTGGAAAGCT TCAGGAGGCC GGGGGCATCC TTGGAGTCCG AGAATGATGG CAGCACCTGG
    AGAAGCAGGT CTCACTCTGA CGAGCTGTTC CAGCCCATGG GCTCGTCCCC CTCCACGGAC
    TCCTTCTTAA CGGAAGATGT CGTGGATTCT TCTCTGTGGA GTGACCTCAG CGGCGATGCC
    CAGGAGTTTG AAGCGGAATC TTGGAGTCTG GTGGTGGACC CTTCATTTTG TAGTCGGCAG
    GAGAAGGATG TCATCCAAAG ACAGGATGTC ATTTTTGAGC TAATGCAGAC AGAACTGCAT
    CACATCCAGA CCCTGTTCAT CATGTCTGAG ATCTTCAGGA AAGGGATGAA AGAGGAGCTG
    CAGCTGGACC ACAGCACTGT GGACAAAATC TTCCCCTGTC TGGACGAGTT ACTTGAAATC
    CACAAGCATT TCTTCTGTAG TATGAAGGAG CGAAGGCAGG AATCCTGTGT TGGCAATGAC
    AGGAATTTCA TCATCGGCCG AATTGGAGAT ATTCTAGTAC AACAGTTTTC AGAAGAAAAT
    GCAAATAAAA TGAAGAAAAT ATATGGGGAA TTCTGTAGCC ATCACAAAGA GGCTGTTAGC
    CTCTTTAAAG AACTCCAGCA GAATAAAAAG TTTCAGAATT TTATTAAGCT CCGAAATAGT
    AACCTTCTGG CTCGACGCCG AGGAATTCCA GAATGCATTC TGCTGGTCAC TCAGCGCATC
    ACAAAATACC CTGTCTTGGT GGAAAGGATA TTGCAGTACA CAAAGGAGAG CACTGAAGAA
    CAGAGAGACT TATGCAAAGC TCTTTGCTTA ATTAAAGACA TGATTGCAGC TGTGGATTTA
    AAAGTCAGCG AATATGAGAA AAAACAAAAA TGGCTTGAGA TCCTAAATAA GATCGAAAAC
    AAAACATACA CCAAGCTCAA AAATGGCCAT GTGTTTAGGA AACAGGCACT GATGAGTAAA
    GAGAGGACCC TGTTATATGA TGGCCTTGTC TATTGGAAAA CTGCTACAGG TCGTTTCAAA
    GATATCCTAG CTCTACTTCT AACTGATGTG CTGCTCTTTT TACAAGAAAA AGACCAGAAA
    TACATCTTTG CAGCTGTTGA TCAGAAGCCA TCAGTTATTT CCCTTCAGAA GCTCATTGCT
    AGGGAAGTTG CGAATGAGGA GAGAGGAATG TTTTTGATCA GCGCTTCATC TGCTGGTCCC
    GAGATGTATG AAATCCATAC CAACTCCAAG GAGGAAAGAA ATACCTGGAT GAGACGGATC
    CAACAGGCAG TAGAAAGTTG CCCAGAAGAA GAAGGGGGAA GGACAAGTGA GTCTGATGAA
    GAGAGGAGGA AAGCTGAAGC CAGAGTGGCG AAGATTCAGC AATGCCAAGA AATACTCAGT
    AACCAAGATC AACAAATTTG CACATATTTG GAAGAGAAAC TGCATATCTA CGCGAAACTT
    GGAGAACTAA GTGGATTTCA GGATGTCCAT CTAGAGCCCC ACCTCCTCAT TCAACCAGAC
    CCCGGAGAGC CTCCCCAGGC AGCCTCATTA CTGGCCGCAG CACTGAAAGA GGCTGAGAGT
    CTGCAGGTGG CAGTGAAGGC TTCCCAGATG AGCGATGCAT GCCAGTCCCC CGAGGAAGGT
    TGTGGAGAAC CTGCTTTGCC GGACGTGCTC CATTCTCAGG ACGCACCAGG ATCGTCTACT
    GCTTCACTAG TCACAGAAGG GACAGGAGGA AGAGGCTGTT GGGATGTGGA TGCCGGGACC
    CAGGGTGTGG GAACCGACCT GGCGGTTTCT GATGCGGGGG AGAAGGTGGA ATATAGAAGT
    TTCCCAGGTT CTTTGCAGTC AGAGATTATA CAAGCGATAC AGAATTTAAC TCGTCTCTTA
    TATAGCCTTC AGGCCGCCTT GACCATTCAG GATAGCCACA TTGAGATCCA CAGGCTGGTT
    CTCCAACAGC GGGAGAGCCT GGCCCCGGGC CCCGCGTTCC GAGGCAGCCC CTTTCAGGAC
    CAGGAGAAGT CCCGCAGCCT GGAGAAGCAG CGGGAGGAGC TGGCCGATAC CCACAAGCTG
    CAGCTCCAGT TCCAGCAAGA CCAGCACCGC TGGCTCCGCA GGTGCGACCA GCAGCAGCGC
    GAGCAGGAGG CCCGGGCCAG CCGGCTGCAG GAGCGCGAGA GGGAGTGCCG GGCGCAGGAG
    GAGCTGCTGC TCAGGAGCCG GGGCGAGCTG GACCAGCAGC TCCAAGACTA CCAGCAGAGC
    CTGGAGCGCC TGCGCGAGGG CCAGCGCCTG GTGGAGAGGG AGCGGGAGAG GATGCGGGCC
    CAGCAGAGCC TGCTGCGCCA CTGGAAGCAC GGGCGGCAGA GCAGCCTTCC CGCCGCGTTT
    TCTCCAGGAG CCCTGGAGGT AATGGAACTT AATCGATCTG AGACTTTACG TCATGAAAAT
    TCATTCTTCA TCAATGAAGC TTTAGTGCAA ATGTCACTCA ACCCTTTGAA CAAACCGCCG
    CCACCAGGTA TCCATCAGGA TGCGGCTTAC CCCCCAGGCA TACCTCATTC TGATTTGGTA
    AGGACTACTG AAAATCAAGT AGACCTCAAG ACGGACGTTT CTCAACCCTC AGATATCAAC
    CATGAACTGT GGATAGCGGC TGGGGCCTGT CATCAGATAC CCCCTCTCCA CCAAAGCAGC
    AAGGATTCTT GTAAAAATGA TGTGAACATC TCTCAGACTG CATACCCACC CCTCCATGAC
    TCAAATCCAC ACAGCCCTCG GCTGCAGACG TTTATAGCAG AAGAAAAGCT AAATCTACAG
    AGAGTGACCA GACAAGACGG GGAAACTGGA AATGGAGCAG AAGAAAATAT TGTTTACCTC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa228083
    Clone ID Related Accession (Same CDS sequence) XM_024981397.1 , XM_024981398.1 , XM_024981399.1
    Accession Version XM_024981398.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5283bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product rho guanine nucleotide exchange factor 28 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGAGCTGA ACTGCAGCGA AGTACCTCTG TATGGGCAGA TGACGGTCTG CGCGAAGTTT 
    GGCAAAAATG TTTATCTTCC TAAAGATGCT GAGTTTTACT TTATTTATAA TGGATCTCAT
    CAGAGACACA TCGTGATTGC AGAGCGCACA GAAGATAATG TTCTCCAGTC CAGCATTCCA
    GGCCACGGGC TTCAGGAGAC GGTCACCGTG TCTGTGTGCC TGTGCTCGGA AGGTTACTCC
    CCGGTGACCA TGGGCTCTGA CTCCGTGACC TACGTGGACA ACATGGCCTG CAGGCTGGCC
    CGTCTGCTGG TCACCCAGGC CGATCGCCTC ACCGCCAGCA GCCACCAGAC CTTGCTGACC
    CCGTTTGCCC TGACGGCGGG GGCATTGCCT GCTCTGGACG AGGAGCTCGT GCTGGCCCTG
    ACTCATCTGG AGTTGCCTCT GGGGTGGACC GTCTTGGGGA ATTCTTCACT GGAAGTTTCT
    CTGCATAGGG AGTCTCTTCT GCACCTGGCT ATGAGATGGG GCCTTGCTAA GCTTTCCCAG
    CTCCTTCTGT GTCTCCCTGG GGGAGCCCAG GCCCTGACTT TACCCAACGA GGAGGGTGCC
    ACGCCATTAG ACTTGGCTTT ACATGGTGGA CATTCCAAGC TGGTTGAAGA CATCACAAAC
    TTCCAGGGCA GGCGTTCCCC AGGTTTCTCC TGCGTGCAGC TCAGCGAAGA TGCCTCTCTG
    CGGTATATTC ACTCATCAGA AACTCTGACC CTGACGCTTA ACCACACAGC CGAGCATTTG
    CTGGAGGCGG ATATTAAACT CTTCAGAAAA TACTTTTGGG ATAGAGCCTT TCTCATCAAG
    GCTCTTGAAG AGCAAGAAGG CACGTCAGAG GAGAAACCAG ATATGCCCTC CAGCGCTGCA
    GAAACTGAAG AAGGTATGCA TGCTCCTTCC CCACCTGGGG AACTCAGAAA GTGCTCAGCA
    TTCTCTAGTC AATTGCGAGT TGAAGTGTGT GGAGAAGGAG AGCAGGAGCC GATGGACTCG
    GAGGCGAGAG TAGGTTGCTC TGGGGCCGCA GGGCCCAGAG TTCCTTCAGC AGCACTGACC
    AAAGAGATTA AGAATTCGGG GTGCAGCAGA TCACCACCCG GGAAGGAGGG TGTAAAGCGT
    GTGAAAAGCC TGGTGGTTCC ACTGAATGAA CATGAGGACC AGGAGAGGCT AGATTTGGAT
    CGTTCCTTTG ATGCCCTCAA AAAATCCAAG CACCCCTCAA CATTGCTTGC TGCTGGCCAG
    CTCTCTGACA TGCTGAATGG TGGTGATGAC GTCTACGCTA ACTGCATGGT GATAGACCAG
    GTTGGTGATT TGGACATTAA CTATATTAAT ATAGAGGAAA TCCCTGCAAA TACCTGTCTT
    GAATCCATGG ATTCTTCTCT GGGGCCTGAG AGCAGCAAAC CCAGCCTTCC TACTGAAGCC
    AGTGCTGGCA CATACCCACC AAATAAGAGG CCAGAAGTGA CGTCACACAC GGAAGCCCAA
    TCATCTTTCA TATCATCTAG TTCGTATGCT TCCAATTTGA ATCTTTCTTT TGGTCTTCGT
    GGATTTGAAA AGGAGCAAAG TCGTCTAAAG AAAAGAAGTT CCAGCCTTGA TGCCTTGGAT
    GCTGACAGTG AAGGAGAGGG CCATTCGGAG CAGTCGCACA ATTGCTACAC TCCCGTGTGT
    CAGAGTTCCT CGAGAACTGG CATTGCTAGC GGGGATGAAT TGGACTCTTT TGAGACCAAC
    ACTGAACCAG ATTTTAATAT CTCCAGGACG GAATCATTTT CTTTATCAAG TAGCCTGCAG
    CTGAAGGAAT CATTGTTTTC TGGAATCCGT TCACGTTCGT ATTCCTGCTC ATCACCCAAA
    ATTTCTTTAG GAAAAACTCG ATTGATGCGT GATTTTACAG TGTGTAATTC AATTGAAGAG
    CAAAGAGCTT ACAGCTTGCC GGAGCCACCG AGAGAACAAA GAATTCAGGA AGAAGAATGG
    GATAAATATA TCATACCTGC CAAATCAGAA TCTGAAAAAT ACAAAGTAAG CCGAACTTTT
    AGCTTCCTCA TGAATAGGAT GACCAGCCCT CGGAATAAAT CAAAGACAAA AGGCAAGGAT
    GCCAAAGACA AAGAGAAACT GACTCGACAT CAGTTTGTCC CTGGAACTTT CTCTGGGGTT
    CTGCAGTGTT TGGTTTGCGA TAAAACGCTC CTGGGGAAAG AGTCATTCCA GTGCTCCAAC
    TGTAATGGAA ACGTACATAA AGTCTGTAAA GATGCTGCCC CTCCGTGTAC CAAGAAATTC
    CAAGACAAAT ATAATAAGAA CAAACCACAG ACCATCCTTG GAAATTCTTC GTTTCGAGAC
    GTCCCATTGC CTGGGCTCTC TCTGCACCCT TCCTCCTCCA TGCCTATCGG GTTGCCAACT
    GGGAGGAAGG AGATGGCAGG GCAAGTGCAT CCCTTGTCCA GAAGTGTCCC AGGCACCACC
    TTGGAAAGCT TCAGGAGGCC GGGGGCATCC TTGGAGTCCG AGAATGATGG CAGCACCTGG
    AGAAGCAGGT CTCACTCTGA CGAGCTGTTC CAGCCCATGG GCTCGTCCCC CTCCACGGAC
    TCCTTCTTAA CGGAAGATGT CGTGGATTCT TCTCTGTGGA GTGACCTCAG CGGCGATGCC
    CAGGAGTTTG AAGCGGAATC TTGGAGTCTG GTGGTGGACC CTTCATTTTG TAGTCGGCAG
    GAGAAGGATG TCATCCAAAG ACAGGATGTC ATTTTTGAGC TAATGCAGAC AGAACTGCAT
    CACATCCAGA CCCTGTTCAT CATGTCTGAG ATCTTCAGGA AAGGGATGAA AGAGGAGCTG
    CAGCTGGACC ACAGCACTGT GGACAAAATC TTCCCCTGTC TGGACGAGTT ACTTGAAATC
    CACAAGCATT TCTTCTGTAG TATGAAGGAG CGAAGGCAGG AATCCTGTGT TGGCAATGAC
    AGGAATTTCA TCATCGGCCG AATTGGAGAT ATTCTAGTAC AACAGTTTTC AGAAGAAAAT
    GCAAATAAAA TGAAGAAAAT ATATGGGGAA TTCTGTAGCC ATCACAAAGA GGCTGTTAGC
    CTCTTTAAAG AACTCCAGCA GAATAAAAAG TTTCAGAATT TTATTAAGCT CCGAAATAGT
    AACCTTCTGG CTCGACGCCG AGGAATTCCA GAATGCATTC TGCTGGTCAC TCAGCGCATC
    ACAAAATACC CTGTCTTGGT GGAAAGGATA TTGCAGTACA CAAAGGAGAG CACTGAAGAA
    CAGAGAGACT TATGCAAAGC TCTTTGCTTA ATTAAAGACA TGATTGCAGC TGTGGATTTA
    AAAGTCAGCG AATATGAGAA AAAACAAAAA TGGCTTGAGA TCCTAAATAA GATCGAAAAC
    AAAACATACA CCAAGCTCAA AAATGGCCAT GTGTTTAGGA AACAGGCACT GATGAGTAAA
    GAGAGGACCC TGTTATATGA TGGCCTTGTC TATTGGAAAA CTGCTACAGG TCGTTTCAAA
    GATATCCTAG CTCTACTTCT AACTGATGTG CTGCTCTTTT TACAAGAAAA AGACCAGAAA
    TACATCTTTG CAGCTGTTGA TCAGAAGCCA TCAGTTATTT CCCTTCAGAA GCTCATTGCT
    AGGGAAGTTG CGAATGAGGA GAGAGGAATG TTTTTGATCA GCGCTTCATC TGCTGGTCCC
    GAGATGTATG AAATCCATAC CAACTCCAAG GAGGAAAGAA ATACCTGGAT GAGACGGATC
    CAACAGGCAG TAGAAAGTTG CCCAGAAGAA GAAGGGGGAA GGACAAGTGA GTCTGATGAA
    GAGAGGAGGA AAGCTGAAGC CAGAGTGGCG AAGATTCAGC AATGCCAAGA AATACTCAGT
    AACCAAGATC AACAAATTTG CACATATTTG GAAGAGAAAC TGCATATCTA CGCGAAACTT
    GGAGAACTAA GTGGATTTCA GGATGTCCAT CTAGAGCCCC ACCTCCTCAT TCAACCAGAC
    CCCGGAGAGC CTCCCCAGGC AGCCTCATTA CTGGCCGCAG CACTGAAAGA GGCTGAGAGT
    CTGCAGGTGG CAGTGAAGGC TTCCCAGATG AGCGATGCAT GCCAGTCCCC CGAGGAAGGT
    TGTGGAGAAC CTGCTTTGCC GGACGTGCTC CATTCTCAGG ACGCACCAGG ATCGTCTACT
    GCTTCACTAG TCACAGAAGG GACAGGAGGA AGAGGCTGTT GGGATGTGGA TGCCGGGACC
    CAGGGTGTGG GAACCGACCT GGCGGTTTCT GATGCGGGGG AGAAGGTGGA ATATAGAAGT
    TTCCCAGGTT CTTTGCAGTC AGAGATTATA CAAGCGATAC AGAATTTAAC TCGTCTCTTA
    TATAGCCTTC AGGCCGCCTT GACCATTCAG GATAGCCACA TTGAGATCCA CAGGCTGGTT
    CTCCAACAGC GGGAGAGCCT GGCCCCGGGC CCCGCGTTCC GAGGCAGCCC CTTTCAGGAC
    CAGGAGAAGT CCCGCAGCCT GGAGAAGCAG CGGGAGGAGC TGGCCGATAC CCACAAGCTG
    CAGCTCCAGT TCCAGCAAGA CCAGCACCGC TGGCTCCGCA GGTGCGACCA GCAGCAGCGC
    GAGCAGGAGG CCCGGGCCAG CCGGCTGCAG GAGCGCGAGA GGGAGTGCCG GGCGCAGGAG
    GAGCTGCTGC TCAGGAGCCG GGGCGAGCTG GACCAGCAGC TCCAAGACTA CCAGCAGAGC
    CTGGAGCGCC TGCGCGAGGG CCAGCGCCTG GTGGAGAGGG AGCGGGAGAG GATGCGGGCC
    CAGCAGAGCC TGCTGCGCCA CTGGAAGCAC GGGCGGCAGA GCAGCCTTCC CGCCGCGTTT
    TCTCCAGGAG CCCTGGAGGT AATGGAACTT AATCGATCTG AGACTTTACG TCATGAAAAT
    TCATTCTTCA TCAATGAAGC TTTAGTGCAA ATGTCACTCA ACCCTTTGAA CAAACCGCCG
    CCACCAGGTA TCCATCAGGA TGCGGCTTAC CCCCCAGGCA TACCTCATTC TGATTTGGTA
    AGGACTACTG AAAATCAAGT AGACCTCAAG ACGGACGTTT CTCAACCCTC AGATATCAAC
    CATGAACTGT GGATAGCGGC TGGGGCCTGT CATCAGATAC CCCCTCTCCA CCAAAGCAGC
    AAGGATTCTT GTAAAAATGA TGTGAACATC TCTCAGACTG CATACCCACC CCTCCATGAC
    TCAAATCCAC ACAGCCCTCG GCTGCAGACG TTTATAGCAG AAGAAAAGCT AAATCTACAG
    AGAGTGACCA GACAAGACGG GGAAACTGGA AATGGAGCAG AAGAAAATAT TGTTTACCTC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024837166.1
    CDS203..5485
    Translation

    Target ORF information:

    RefSeq Version XM_024981398.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981398.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGAGCTGA ACTGCAGCGA AGTACCTCTG TATGGGCAGA TGACGGTCTG CGCGAAGTTT 
    GGCAAAAATG TTTATCTTCC TAAAGATGCT GAGTTTTACT TTATTTATAA TGGATCTCAT
    CAGAGACACA TCGTGATTGC AGAGCGCACA GAAGATAATG TTCTCCAGTC CAGCATTCCA
    GGCCACGGGC TTCAGGAGAC GGTCACCGTG TCTGTGTGCC TGTGCTCGGA AGGTTACTCC
    CCGGTGACCA TGGGCTCTGA CTCCGTGACC TACGTGGACA ACATGGCCTG CAGGCTGGCC
    CGTCTGCTGG TCACCCAGGC CGATCGCCTC ACCGCCAGCA GCCACCAGAC CTTGCTGACC
    CCGTTTGCCC TGACGGCGGG GGCATTGCCT GCTCTGGACG AGGAGCTCGT GCTGGCCCTG
    ACTCATCTGG AGTTGCCTCT GGGGTGGACC GTCTTGGGGA ATTCTTCACT GGAAGTTTCT
    CTGCATAGGG AGTCTCTTCT GCACCTGGCT ATGAGATGGG GCCTTGCTAA GCTTTCCCAG
    CTCCTTCTGT GTCTCCCTGG GGGAGCCCAG GCCCTGACTT TACCCAACGA GGAGGGTGCC
    ACGCCATTAG ACTTGGCTTT ACATGGTGGA CATTCCAAGC TGGTTGAAGA CATCACAAAC
    TTCCAGGGCA GGCGTTCCCC AGGTTTCTCC TGCGTGCAGC TCAGCGAAGA TGCCTCTCTG
    CGGTATATTC ACTCATCAGA AACTCTGACC CTGACGCTTA ACCACACAGC CGAGCATTTG
    CTGGAGGCGG ATATTAAACT CTTCAGAAAA TACTTTTGGG ATAGAGCCTT TCTCATCAAG
    GCTCTTGAAG AGCAAGAAGG CACGTCAGAG GAGAAACCAG ATATGCCCTC CAGCGCTGCA
    GAAACTGAAG AAGGTATGCA TGCTCCTTCC CCACCTGGGG AACTCAGAAA GTGCTCAGCA
    TTCTCTAGTC AATTGCGAGT TGAAGTGTGT GGAGAAGGAG AGCAGGAGCC GATGGACTCG
    GAGGCGAGAG TAGGTTGCTC TGGGGCCGCA GGGCCCAGAG TTCCTTCAGC AGCACTGACC
    AAAGAGATTA AGAATTCGGG GTGCAGCAGA TCACCACCCG GGAAGGAGGG TGTAAAGCGT
    GTGAAAAGCC TGGTGGTTCC ACTGAATGAA CATGAGGACC AGGAGAGGCT AGATTTGGAT
    CGTTCCTTTG ATGCCCTCAA AAAATCCAAG CACCCCTCAA CATTGCTTGC TGCTGGCCAG
    CTCTCTGACA TGCTGAATGG TGGTGATGAC GTCTACGCTA ACTGCATGGT GATAGACCAG
    GTTGGTGATT TGGACATTAA CTATATTAAT ATAGAGGAAA TCCCTGCAAA TACCTGTCTT
    GAATCCATGG ATTCTTCTCT GGGGCCTGAG AGCAGCAAAC CCAGCCTTCC TACTGAAGCC
    AGTGCTGGCA CATACCCACC AAATAAGAGG CCAGAAGTGA CGTCACACAC GGAAGCCCAA
    TCATCTTTCA TATCATCTAG TTCGTATGCT TCCAATTTGA ATCTTTCTTT TGGTCTTCGT
    GGATTTGAAA AGGAGCAAAG TCGTCTAAAG AAAAGAAGTT CCAGCCTTGA TGCCTTGGAT
    GCTGACAGTG AAGGAGAGGG CCATTCGGAG CAGTCGCACA ATTGCTACAC TCCCGTGTGT
    CAGAGTTCCT CGAGAACTGG CATTGCTAGC GGGGATGAAT TGGACTCTTT TGAGACCAAC
    ACTGAACCAG ATTTTAATAT CTCCAGGACG GAATCATTTT CTTTATCAAG TAGCCTGCAG
    CTGAAGGAAT CATTGTTTTC TGGAATCCGT TCACGTTCGT ATTCCTGCTC ATCACCCAAA
    ATTTCTTTAG GAAAAACTCG ATTGATGCGT GATTTTACAG TGTGTAATTC AATTGAAGAG
    CAAAGAGCTT ACAGCTTGCC GGAGCCACCG AGAGAACAAA GAATTCAGGA AGAAGAATGG
    GATAAATATA TCATACCTGC CAAATCAGAA TCTGAAAAAT ACAAAGTAAG CCGAACTTTT
    AGCTTCCTCA TGAATAGGAT GACCAGCCCT CGGAATAAAT CAAAGACAAA AGGCAAGGAT
    GCCAAAGACA AAGAGAAACT GACTCGACAT CAGTTTGTCC CTGGAACTTT CTCTGGGGTT
    CTGCAGTGTT TGGTTTGCGA TAAAACGCTC CTGGGGAAAG AGTCATTCCA GTGCTCCAAC
    TGTAATGGAA ACGTACATAA AGTCTGTAAA GATGCTGCCC CTCCGTGTAC CAAGAAATTC
    CAAGACAAAT ATAATAAGAA CAAACCACAG ACCATCCTTG GAAATTCTTC GTTTCGAGAC
    GTCCCATTGC CTGGGCTCTC TCTGCACCCT TCCTCCTCCA TGCCTATCGG GTTGCCAACT
    GGGAGGAAGG AGATGGCAGG GCAAGTGCAT CCCTTGTCCA GAAGTGTCCC AGGCACCACC
    TTGGAAAGCT TCAGGAGGCC GGGGGCATCC TTGGAGTCCG AGAATGATGG CAGCACCTGG
    AGAAGCAGGT CTCACTCTGA CGAGCTGTTC CAGCCCATGG GCTCGTCCCC CTCCACGGAC
    TCCTTCTTAA CGGAAGATGT CGTGGATTCT TCTCTGTGGA GTGACCTCAG CGGCGATGCC
    CAGGAGTTTG AAGCGGAATC TTGGAGTCTG GTGGTGGACC CTTCATTTTG TAGTCGGCAG
    GAGAAGGATG TCATCCAAAG ACAGGATGTC ATTTTTGAGC TAATGCAGAC AGAACTGCAT
    CACATCCAGA CCCTGTTCAT CATGTCTGAG ATCTTCAGGA AAGGGATGAA AGAGGAGCTG
    CAGCTGGACC ACAGCACTGT GGACAAAATC TTCCCCTGTC TGGACGAGTT ACTTGAAATC
    CACAAGCATT TCTTCTGTAG TATGAAGGAG CGAAGGCAGG AATCCTGTGT TGGCAATGAC
    AGGAATTTCA TCATCGGCCG AATTGGAGAT ATTCTAGTAC AACAGTTTTC AGAAGAAAAT
    GCAAATAAAA TGAAGAAAAT ATATGGGGAA TTCTGTAGCC ATCACAAAGA GGCTGTTAGC
    CTCTTTAAAG AACTCCAGCA GAATAAAAAG TTTCAGAATT TTATTAAGCT CCGAAATAGT
    AACCTTCTGG CTCGACGCCG AGGAATTCCA GAATGCATTC TGCTGGTCAC TCAGCGCATC
    ACAAAATACC CTGTCTTGGT GGAAAGGATA TTGCAGTACA CAAAGGAGAG CACTGAAGAA
    CAGAGAGACT TATGCAAAGC TCTTTGCTTA ATTAAAGACA TGATTGCAGC TGTGGATTTA
    AAAGTCAGCG AATATGAGAA AAAACAAAAA TGGCTTGAGA TCCTAAATAA GATCGAAAAC
    AAAACATACA CCAAGCTCAA AAATGGCCAT GTGTTTAGGA AACAGGCACT GATGAGTAAA
    GAGAGGACCC TGTTATATGA TGGCCTTGTC TATTGGAAAA CTGCTACAGG TCGTTTCAAA
    GATATCCTAG CTCTACTTCT AACTGATGTG CTGCTCTTTT TACAAGAAAA AGACCAGAAA
    TACATCTTTG CAGCTGTTGA TCAGAAGCCA TCAGTTATTT CCCTTCAGAA GCTCATTGCT
    AGGGAAGTTG CGAATGAGGA GAGAGGAATG TTTTTGATCA GCGCTTCATC TGCTGGTCCC
    GAGATGTATG AAATCCATAC CAACTCCAAG GAGGAAAGAA ATACCTGGAT GAGACGGATC
    CAACAGGCAG TAGAAAGTTG CCCAGAAGAA GAAGGGGGAA GGACAAGTGA GTCTGATGAA
    GAGAGGAGGA AAGCTGAAGC CAGAGTGGCG AAGATTCAGC AATGCCAAGA AATACTCAGT
    AACCAAGATC AACAAATTTG CACATATTTG GAAGAGAAAC TGCATATCTA CGCGAAACTT
    GGAGAACTAA GTGGATTTCA GGATGTCCAT CTAGAGCCCC ACCTCCTCAT TCAACCAGAC
    CCCGGAGAGC CTCCCCAGGC AGCCTCATTA CTGGCCGCAG CACTGAAAGA GGCTGAGAGT
    CTGCAGGTGG CAGTGAAGGC TTCCCAGATG AGCGATGCAT GCCAGTCCCC CGAGGAAGGT
    TGTGGAGAAC CTGCTTTGCC GGACGTGCTC CATTCTCAGG ACGCACCAGG ATCGTCTACT
    GCTTCACTAG TCACAGAAGG GACAGGAGGA AGAGGCTGTT GGGATGTGGA TGCCGGGACC
    CAGGGTGTGG GAACCGACCT GGCGGTTTCT GATGCGGGGG AGAAGGTGGA ATATAGAAGT
    TTCCCAGGTT CTTTGCAGTC AGAGATTATA CAAGCGATAC AGAATTTAAC TCGTCTCTTA
    TATAGCCTTC AGGCCGCCTT GACCATTCAG GATAGCCACA TTGAGATCCA CAGGCTGGTT
    CTCCAACAGC GGGAGAGCCT GGCCCCGGGC CCCGCGTTCC GAGGCAGCCC CTTTCAGGAC
    CAGGAGAAGT CCCGCAGCCT GGAGAAGCAG CGGGAGGAGC TGGCCGATAC CCACAAGCTG
    CAGCTCCAGT TCCAGCAAGA CCAGCACCGC TGGCTCCGCA GGTGCGACCA GCAGCAGCGC
    GAGCAGGAGG CCCGGGCCAG CCGGCTGCAG GAGCGCGAGA GGGAGTGCCG GGCGCAGGAG
    GAGCTGCTGC TCAGGAGCCG GGGCGAGCTG GACCAGCAGC TCCAAGACTA CCAGCAGAGC
    CTGGAGCGCC TGCGCGAGGG CCAGCGCCTG GTGGAGAGGG AGCGGGAGAG GATGCGGGCC
    CAGCAGAGCC TGCTGCGCCA CTGGAAGCAC GGGCGGCAGA GCAGCCTTCC CGCCGCGTTT
    TCTCCAGGAG CCCTGGAGGT AATGGAACTT AATCGATCTG AGACTTTACG TCATGAAAAT
    TCATTCTTCA TCAATGAAGC TTTAGTGCAA ATGTCACTCA ACCCTTTGAA CAAACCGCCG
    CCACCAGGTA TCCATCAGGA TGCGGCTTAC CCCCCAGGCA TACCTCATTC TGATTTGGTA
    AGGACTACTG AAAATCAAGT AGACCTCAAG ACGGACGTTT CTCAACCCTC AGATATCAAC
    CATGAACTGT GGATAGCGGC TGGGGCCTGT CATCAGATAC CCCCTCTCCA CCAAAGCAGC
    AAGGATTCTT GTAAAAATGA TGTGAACATC TCTCAGACTG CATACCCACC CCTCCATGAC
    TCAAATCCAC ACAGCCCTCG GCTGCAGACG TTTATAGCAG AAGAAAAGCT AAATCTACAG
    AGAGTGACCA GACAAGACGG GGAAACTGGA AATGGAGCAG AAGAAAATAT TGTTTACCTC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa228083
    Clone ID Related Accession (Same CDS sequence) XM_024981397.1 , XM_024981398.1 , XM_024981399.1
    Accession Version XM_024981399.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5283bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product rho guanine nucleotide exchange factor 28 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGAGCTGA ACTGCAGCGA AGTACCTCTG TATGGGCAGA TGACGGTCTG CGCGAAGTTT 
    GGCAAAAATG TTTATCTTCC TAAAGATGCT GAGTTTTACT TTATTTATAA TGGATCTCAT
    CAGAGACACA TCGTGATTGC AGAGCGCACA GAAGATAATG TTCTCCAGTC CAGCATTCCA
    GGCCACGGGC TTCAGGAGAC GGTCACCGTG TCTGTGTGCC TGTGCTCGGA AGGTTACTCC
    CCGGTGACCA TGGGCTCTGA CTCCGTGACC TACGTGGACA ACATGGCCTG CAGGCTGGCC
    CGTCTGCTGG TCACCCAGGC CGATCGCCTC ACCGCCAGCA GCCACCAGAC CTTGCTGACC
    CCGTTTGCCC TGACGGCGGG GGCATTGCCT GCTCTGGACG AGGAGCTCGT GCTGGCCCTG
    ACTCATCTGG AGTTGCCTCT GGGGTGGACC GTCTTGGGGA ATTCTTCACT GGAAGTTTCT
    CTGCATAGGG AGTCTCTTCT GCACCTGGCT ATGAGATGGG GCCTTGCTAA GCTTTCCCAG
    CTCCTTCTGT GTCTCCCTGG GGGAGCCCAG GCCCTGACTT TACCCAACGA GGAGGGTGCC
    ACGCCATTAG ACTTGGCTTT ACATGGTGGA CATTCCAAGC TGGTTGAAGA CATCACAAAC
    TTCCAGGGCA GGCGTTCCCC AGGTTTCTCC TGCGTGCAGC TCAGCGAAGA TGCCTCTCTG
    CGGTATATTC ACTCATCAGA AACTCTGACC CTGACGCTTA ACCACACAGC CGAGCATTTG
    CTGGAGGCGG ATATTAAACT CTTCAGAAAA TACTTTTGGG ATAGAGCCTT TCTCATCAAG
    GCTCTTGAAG AGCAAGAAGG CACGTCAGAG GAGAAACCAG ATATGCCCTC CAGCGCTGCA
    GAAACTGAAG AAGGTATGCA TGCTCCTTCC CCACCTGGGG AACTCAGAAA GTGCTCAGCA
    TTCTCTAGTC AATTGCGAGT TGAAGTGTGT GGAGAAGGAG AGCAGGAGCC GATGGACTCG
    GAGGCGAGAG TAGGTTGCTC TGGGGCCGCA GGGCCCAGAG TTCCTTCAGC AGCACTGACC
    AAAGAGATTA AGAATTCGGG GTGCAGCAGA TCACCACCCG GGAAGGAGGG TGTAAAGCGT
    GTGAAAAGCC TGGTGGTTCC ACTGAATGAA CATGAGGACC AGGAGAGGCT AGATTTGGAT
    CGTTCCTTTG ATGCCCTCAA AAAATCCAAG CACCCCTCAA CATTGCTTGC TGCTGGCCAG
    CTCTCTGACA TGCTGAATGG TGGTGATGAC GTCTACGCTA ACTGCATGGT GATAGACCAG
    GTTGGTGATT TGGACATTAA CTATATTAAT ATAGAGGAAA TCCCTGCAAA TACCTGTCTT
    GAATCCATGG ATTCTTCTCT GGGGCCTGAG AGCAGCAAAC CCAGCCTTCC TACTGAAGCC
    AGTGCTGGCA CATACCCACC AAATAAGAGG CCAGAAGTGA CGTCACACAC GGAAGCCCAA
    TCATCTTTCA TATCATCTAG TTCGTATGCT TCCAATTTGA ATCTTTCTTT TGGTCTTCGT
    GGATTTGAAA AGGAGCAAAG TCGTCTAAAG AAAAGAAGTT CCAGCCTTGA TGCCTTGGAT
    GCTGACAGTG AAGGAGAGGG CCATTCGGAG CAGTCGCACA ATTGCTACAC TCCCGTGTGT
    CAGAGTTCCT CGAGAACTGG CATTGCTAGC GGGGATGAAT TGGACTCTTT TGAGACCAAC
    ACTGAACCAG ATTTTAATAT CTCCAGGACG GAATCATTTT CTTTATCAAG TAGCCTGCAG
    CTGAAGGAAT CATTGTTTTC TGGAATCCGT TCACGTTCGT ATTCCTGCTC ATCACCCAAA
    ATTTCTTTAG GAAAAACTCG ATTGATGCGT GATTTTACAG TGTGTAATTC AATTGAAGAG
    CAAAGAGCTT ACAGCTTGCC GGAGCCACCG AGAGAACAAA GAATTCAGGA AGAAGAATGG
    GATAAATATA TCATACCTGC CAAATCAGAA TCTGAAAAAT ACAAAGTAAG CCGAACTTTT
    AGCTTCCTCA TGAATAGGAT GACCAGCCCT CGGAATAAAT CAAAGACAAA AGGCAAGGAT
    GCCAAAGACA AAGAGAAACT GACTCGACAT CAGTTTGTCC CTGGAACTTT CTCTGGGGTT
    CTGCAGTGTT TGGTTTGCGA TAAAACGCTC CTGGGGAAAG AGTCATTCCA GTGCTCCAAC
    TGTAATGGAA ACGTACATAA AGTCTGTAAA GATGCTGCCC CTCCGTGTAC CAAGAAATTC
    CAAGACAAAT ATAATAAGAA CAAACCACAG ACCATCCTTG GAAATTCTTC GTTTCGAGAC
    GTCCCATTGC CTGGGCTCTC TCTGCACCCT TCCTCCTCCA TGCCTATCGG GTTGCCAACT
    GGGAGGAAGG AGATGGCAGG GCAAGTGCAT CCCTTGTCCA GAAGTGTCCC AGGCACCACC
    TTGGAAAGCT TCAGGAGGCC GGGGGCATCC TTGGAGTCCG AGAATGATGG CAGCACCTGG
    AGAAGCAGGT CTCACTCTGA CGAGCTGTTC CAGCCCATGG GCTCGTCCCC CTCCACGGAC
    TCCTTCTTAA CGGAAGATGT CGTGGATTCT TCTCTGTGGA GTGACCTCAG CGGCGATGCC
    CAGGAGTTTG AAGCGGAATC TTGGAGTCTG GTGGTGGACC CTTCATTTTG TAGTCGGCAG
    GAGAAGGATG TCATCCAAAG ACAGGATGTC ATTTTTGAGC TAATGCAGAC AGAACTGCAT
    CACATCCAGA CCCTGTTCAT CATGTCTGAG ATCTTCAGGA AAGGGATGAA AGAGGAGCTG
    CAGCTGGACC ACAGCACTGT GGACAAAATC TTCCCCTGTC TGGACGAGTT ACTTGAAATC
    CACAAGCATT TCTTCTGTAG TATGAAGGAG CGAAGGCAGG AATCCTGTGT TGGCAATGAC
    AGGAATTTCA TCATCGGCCG AATTGGAGAT ATTCTAGTAC AACAGTTTTC AGAAGAAAAT
    GCAAATAAAA TGAAGAAAAT ATATGGGGAA TTCTGTAGCC ATCACAAAGA GGCTGTTAGC
    CTCTTTAAAG AACTCCAGCA GAATAAAAAG TTTCAGAATT TTATTAAGCT CCGAAATAGT
    AACCTTCTGG CTCGACGCCG AGGAATTCCA GAATGCATTC TGCTGGTCAC TCAGCGCATC
    ACAAAATACC CTGTCTTGGT GGAAAGGATA TTGCAGTACA CAAAGGAGAG CACTGAAGAA
    CAGAGAGACT TATGCAAAGC TCTTTGCTTA ATTAAAGACA TGATTGCAGC TGTGGATTTA
    AAAGTCAGCG AATATGAGAA AAAACAAAAA TGGCTTGAGA TCCTAAATAA GATCGAAAAC
    AAAACATACA CCAAGCTCAA AAATGGCCAT GTGTTTAGGA AACAGGCACT GATGAGTAAA
    GAGAGGACCC TGTTATATGA TGGCCTTGTC TATTGGAAAA CTGCTACAGG TCGTTTCAAA
    GATATCCTAG CTCTACTTCT AACTGATGTG CTGCTCTTTT TACAAGAAAA AGACCAGAAA
    TACATCTTTG CAGCTGTTGA TCAGAAGCCA TCAGTTATTT CCCTTCAGAA GCTCATTGCT
    AGGGAAGTTG CGAATGAGGA GAGAGGAATG TTTTTGATCA GCGCTTCATC TGCTGGTCCC
    GAGATGTATG AAATCCATAC CAACTCCAAG GAGGAAAGAA ATACCTGGAT GAGACGGATC
    CAACAGGCAG TAGAAAGTTG CCCAGAAGAA GAAGGGGGAA GGACAAGTGA GTCTGATGAA
    GAGAGGAGGA AAGCTGAAGC CAGAGTGGCG AAGATTCAGC AATGCCAAGA AATACTCAGT
    AACCAAGATC AACAAATTTG CACATATTTG GAAGAGAAAC TGCATATCTA CGCGAAACTT
    GGAGAACTAA GTGGATTTCA GGATGTCCAT CTAGAGCCCC ACCTCCTCAT TCAACCAGAC
    CCCGGAGAGC CTCCCCAGGC AGCCTCATTA CTGGCCGCAG CACTGAAAGA GGCTGAGAGT
    CTGCAGGTGG CAGTGAAGGC TTCCCAGATG AGCGATGCAT GCCAGTCCCC CGAGGAAGGT
    TGTGGAGAAC CTGCTTTGCC GGACGTGCTC CATTCTCAGG ACGCACCAGG ATCGTCTACT
    GCTTCACTAG TCACAGAAGG GACAGGAGGA AGAGGCTGTT GGGATGTGGA TGCCGGGACC
    CAGGGTGTGG GAACCGACCT GGCGGTTTCT GATGCGGGGG AGAAGGTGGA ATATAGAAGT
    TTCCCAGGTT CTTTGCAGTC AGAGATTATA CAAGCGATAC AGAATTTAAC TCGTCTCTTA
    TATAGCCTTC AGGCCGCCTT GACCATTCAG GATAGCCACA TTGAGATCCA CAGGCTGGTT
    CTCCAACAGC GGGAGAGCCT GGCCCCGGGC CCCGCGTTCC GAGGCAGCCC CTTTCAGGAC
    CAGGAGAAGT CCCGCAGCCT GGAGAAGCAG CGGGAGGAGC TGGCCGATAC CCACAAGCTG
    CAGCTCCAGT TCCAGCAAGA CCAGCACCGC TGGCTCCGCA GGTGCGACCA GCAGCAGCGC
    GAGCAGGAGG CCCGGGCCAG CCGGCTGCAG GAGCGCGAGA GGGAGTGCCG GGCGCAGGAG
    GAGCTGCTGC TCAGGAGCCG GGGCGAGCTG GACCAGCAGC TCCAAGACTA CCAGCAGAGC
    CTGGAGCGCC TGCGCGAGGG CCAGCGCCTG GTGGAGAGGG AGCGGGAGAG GATGCGGGCC
    CAGCAGAGCC TGCTGCGCCA CTGGAAGCAC GGGCGGCAGA GCAGCCTTCC CGCCGCGTTT
    TCTCCAGGAG CCCTGGAGGT AATGGAACTT AATCGATCTG AGACTTTACG TCATGAAAAT
    TCATTCTTCA TCAATGAAGC TTTAGTGCAA ATGTCACTCA ACCCTTTGAA CAAACCGCCG
    CCACCAGGTA TCCATCAGGA TGCGGCTTAC CCCCCAGGCA TACCTCATTC TGATTTGGTA
    AGGACTACTG AAAATCAAGT AGACCTCAAG ACGGACGTTT CTCAACCCTC AGATATCAAC
    CATGAACTGT GGATAGCGGC TGGGGCCTGT CATCAGATAC CCCCTCTCCA CCAAAGCAGC
    AAGGATTCTT GTAAAAATGA TGTGAACATC TCTCAGACTG CATACCCACC CCTCCATGAC
    TCAAATCCAC ACAGCCCTCG GCTGCAGACG TTTATAGCAG AAGAAAAGCT AAATCTACAG
    AGAGTGACCA GACAAGACGG GGAAACTGGA AATGGAGCAG AAGAAAATAT TGTTTACCTC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024837167.1
    CDS232..5514
    Translation

    Target ORF information:

    RefSeq Version XM_024981399.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981399.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGAGCTGA ACTGCAGCGA AGTACCTCTG TATGGGCAGA TGACGGTCTG CGCGAAGTTT 
    GGCAAAAATG TTTATCTTCC TAAAGATGCT GAGTTTTACT TTATTTATAA TGGATCTCAT
    CAGAGACACA TCGTGATTGC AGAGCGCACA GAAGATAATG TTCTCCAGTC CAGCATTCCA
    GGCCACGGGC TTCAGGAGAC GGTCACCGTG TCTGTGTGCC TGTGCTCGGA AGGTTACTCC
    CCGGTGACCA TGGGCTCTGA CTCCGTGACC TACGTGGACA ACATGGCCTG CAGGCTGGCC
    CGTCTGCTGG TCACCCAGGC CGATCGCCTC ACCGCCAGCA GCCACCAGAC CTTGCTGACC
    CCGTTTGCCC TGACGGCGGG GGCATTGCCT GCTCTGGACG AGGAGCTCGT GCTGGCCCTG
    ACTCATCTGG AGTTGCCTCT GGGGTGGACC GTCTTGGGGA ATTCTTCACT GGAAGTTTCT
    CTGCATAGGG AGTCTCTTCT GCACCTGGCT ATGAGATGGG GCCTTGCTAA GCTTTCCCAG
    CTCCTTCTGT GTCTCCCTGG GGGAGCCCAG GCCCTGACTT TACCCAACGA GGAGGGTGCC
    ACGCCATTAG ACTTGGCTTT ACATGGTGGA CATTCCAAGC TGGTTGAAGA CATCACAAAC
    TTCCAGGGCA GGCGTTCCCC AGGTTTCTCC TGCGTGCAGC TCAGCGAAGA TGCCTCTCTG
    CGGTATATTC ACTCATCAGA AACTCTGACC CTGACGCTTA ACCACACAGC CGAGCATTTG
    CTGGAGGCGG ATATTAAACT CTTCAGAAAA TACTTTTGGG ATAGAGCCTT TCTCATCAAG
    GCTCTTGAAG AGCAAGAAGG CACGTCAGAG GAGAAACCAG ATATGCCCTC CAGCGCTGCA
    GAAACTGAAG AAGGTATGCA TGCTCCTTCC CCACCTGGGG AACTCAGAAA GTGCTCAGCA
    TTCTCTAGTC AATTGCGAGT TGAAGTGTGT GGAGAAGGAG AGCAGGAGCC GATGGACTCG
    GAGGCGAGAG TAGGTTGCTC TGGGGCCGCA GGGCCCAGAG TTCCTTCAGC AGCACTGACC
    AAAGAGATTA AGAATTCGGG GTGCAGCAGA TCACCACCCG GGAAGGAGGG TGTAAAGCGT
    GTGAAAAGCC TGGTGGTTCC ACTGAATGAA CATGAGGACC AGGAGAGGCT AGATTTGGAT
    CGTTCCTTTG ATGCCCTCAA AAAATCCAAG CACCCCTCAA CATTGCTTGC TGCTGGCCAG
    CTCTCTGACA TGCTGAATGG TGGTGATGAC GTCTACGCTA ACTGCATGGT GATAGACCAG
    GTTGGTGATT TGGACATTAA CTATATTAAT ATAGAGGAAA TCCCTGCAAA TACCTGTCTT
    GAATCCATGG ATTCTTCTCT GGGGCCTGAG AGCAGCAAAC CCAGCCTTCC TACTGAAGCC
    AGTGCTGGCA CATACCCACC AAATAAGAGG CCAGAAGTGA CGTCACACAC GGAAGCCCAA
    TCATCTTTCA TATCATCTAG TTCGTATGCT TCCAATTTGA ATCTTTCTTT TGGTCTTCGT
    GGATTTGAAA AGGAGCAAAG TCGTCTAAAG AAAAGAAGTT CCAGCCTTGA TGCCTTGGAT
    GCTGACAGTG AAGGAGAGGG CCATTCGGAG CAGTCGCACA ATTGCTACAC TCCCGTGTGT
    CAGAGTTCCT CGAGAACTGG CATTGCTAGC GGGGATGAAT TGGACTCTTT TGAGACCAAC
    ACTGAACCAG ATTTTAATAT CTCCAGGACG GAATCATTTT CTTTATCAAG TAGCCTGCAG
    CTGAAGGAAT CATTGTTTTC TGGAATCCGT TCACGTTCGT ATTCCTGCTC ATCACCCAAA
    ATTTCTTTAG GAAAAACTCG ATTGATGCGT GATTTTACAG TGTGTAATTC AATTGAAGAG
    CAAAGAGCTT ACAGCTTGCC GGAGCCACCG AGAGAACAAA GAATTCAGGA AGAAGAATGG
    GATAAATATA TCATACCTGC CAAATCAGAA TCTGAAAAAT ACAAAGTAAG CCGAACTTTT
    AGCTTCCTCA TGAATAGGAT GACCAGCCCT CGGAATAAAT CAAAGACAAA AGGCAAGGAT
    GCCAAAGACA AAGAGAAACT GACTCGACAT CAGTTTGTCC CTGGAACTTT CTCTGGGGTT
    CTGCAGTGTT TGGTTTGCGA TAAAACGCTC CTGGGGAAAG AGTCATTCCA GTGCTCCAAC
    TGTAATGGAA ACGTACATAA AGTCTGTAAA GATGCTGCCC CTCCGTGTAC CAAGAAATTC
    CAAGACAAAT ATAATAAGAA CAAACCACAG ACCATCCTTG GAAATTCTTC GTTTCGAGAC
    GTCCCATTGC CTGGGCTCTC TCTGCACCCT TCCTCCTCCA TGCCTATCGG GTTGCCAACT
    GGGAGGAAGG AGATGGCAGG GCAAGTGCAT CCCTTGTCCA GAAGTGTCCC AGGCACCACC
    TTGGAAAGCT TCAGGAGGCC GGGGGCATCC TTGGAGTCCG AGAATGATGG CAGCACCTGG
    AGAAGCAGGT CTCACTCTGA CGAGCTGTTC CAGCCCATGG GCTCGTCCCC CTCCACGGAC
    TCCTTCTTAA CGGAAGATGT CGTGGATTCT TCTCTGTGGA GTGACCTCAG CGGCGATGCC
    CAGGAGTTTG AAGCGGAATC TTGGAGTCTG GTGGTGGACC CTTCATTTTG TAGTCGGCAG
    GAGAAGGATG TCATCCAAAG ACAGGATGTC ATTTTTGAGC TAATGCAGAC AGAACTGCAT
    CACATCCAGA CCCTGTTCAT CATGTCTGAG ATCTTCAGGA AAGGGATGAA AGAGGAGCTG
    CAGCTGGACC ACAGCACTGT GGACAAAATC TTCCCCTGTC TGGACGAGTT ACTTGAAATC
    CACAAGCATT TCTTCTGTAG TATGAAGGAG CGAAGGCAGG AATCCTGTGT TGGCAATGAC
    AGGAATTTCA TCATCGGCCG AATTGGAGAT ATTCTAGTAC AACAGTTTTC AGAAGAAAAT
    GCAAATAAAA TGAAGAAAAT ATATGGGGAA TTCTGTAGCC ATCACAAAGA GGCTGTTAGC
    CTCTTTAAAG AACTCCAGCA GAATAAAAAG TTTCAGAATT TTATTAAGCT CCGAAATAGT
    AACCTTCTGG CTCGACGCCG AGGAATTCCA GAATGCATTC TGCTGGTCAC TCAGCGCATC
    ACAAAATACC CTGTCTTGGT GGAAAGGATA TTGCAGTACA CAAAGGAGAG CACTGAAGAA
    CAGAGAGACT TATGCAAAGC TCTTTGCTTA ATTAAAGACA TGATTGCAGC TGTGGATTTA
    AAAGTCAGCG AATATGAGAA AAAACAAAAA TGGCTTGAGA TCCTAAATAA GATCGAAAAC
    AAAACATACA CCAAGCTCAA AAATGGCCAT GTGTTTAGGA AACAGGCACT GATGAGTAAA
    GAGAGGACCC TGTTATATGA TGGCCTTGTC TATTGGAAAA CTGCTACAGG TCGTTTCAAA
    GATATCCTAG CTCTACTTCT AACTGATGTG CTGCTCTTTT TACAAGAAAA AGACCAGAAA
    TACATCTTTG CAGCTGTTGA TCAGAAGCCA TCAGTTATTT CCCTTCAGAA GCTCATTGCT
    AGGGAAGTTG CGAATGAGGA GAGAGGAATG TTTTTGATCA GCGCTTCATC TGCTGGTCCC
    GAGATGTATG AAATCCATAC CAACTCCAAG GAGGAAAGAA ATACCTGGAT GAGACGGATC
    CAACAGGCAG TAGAAAGTTG CCCAGAAGAA GAAGGGGGAA GGACAAGTGA GTCTGATGAA
    GAGAGGAGGA AAGCTGAAGC CAGAGTGGCG AAGATTCAGC AATGCCAAGA AATACTCAGT
    AACCAAGATC AACAAATTTG CACATATTTG GAAGAGAAAC TGCATATCTA CGCGAAACTT
    GGAGAACTAA GTGGATTTCA GGATGTCCAT CTAGAGCCCC ACCTCCTCAT TCAACCAGAC
    CCCGGAGAGC CTCCCCAGGC AGCCTCATTA CTGGCCGCAG CACTGAAAGA GGCTGAGAGT
    CTGCAGGTGG CAGTGAAGGC TTCCCAGATG AGCGATGCAT GCCAGTCCCC CGAGGAAGGT
    TGTGGAGAAC CTGCTTTGCC GGACGTGCTC CATTCTCAGG ACGCACCAGG ATCGTCTACT
    GCTTCACTAG TCACAGAAGG GACAGGAGGA AGAGGCTGTT GGGATGTGGA TGCCGGGACC
    CAGGGTGTGG GAACCGACCT GGCGGTTTCT GATGCGGGGG AGAAGGTGGA ATATAGAAGT
    TTCCCAGGTT CTTTGCAGTC AGAGATTATA CAAGCGATAC AGAATTTAAC TCGTCTCTTA
    TATAGCCTTC AGGCCGCCTT GACCATTCAG GATAGCCACA TTGAGATCCA CAGGCTGGTT
    CTCCAACAGC GGGAGAGCCT GGCCCCGGGC CCCGCGTTCC GAGGCAGCCC CTTTCAGGAC
    CAGGAGAAGT CCCGCAGCCT GGAGAAGCAG CGGGAGGAGC TGGCCGATAC CCACAAGCTG
    CAGCTCCAGT TCCAGCAAGA CCAGCACCGC TGGCTCCGCA GGTGCGACCA GCAGCAGCGC
    GAGCAGGAGG CCCGGGCCAG CCGGCTGCAG GAGCGCGAGA GGGAGTGCCG GGCGCAGGAG
    GAGCTGCTGC TCAGGAGCCG GGGCGAGCTG GACCAGCAGC TCCAAGACTA CCAGCAGAGC
    CTGGAGCGCC TGCGCGAGGG CCAGCGCCTG GTGGAGAGGG AGCGGGAGAG GATGCGGGCC
    CAGCAGAGCC TGCTGCGCCA CTGGAAGCAC GGGCGGCAGA GCAGCCTTCC CGCCGCGTTT
    TCTCCAGGAG CCCTGGAGGT AATGGAACTT AATCGATCTG AGACTTTACG TCATGAAAAT
    TCATTCTTCA TCAATGAAGC TTTAGTGCAA ATGTCACTCA ACCCTTTGAA CAAACCGCCG
    CCACCAGGTA TCCATCAGGA TGCGGCTTAC CCCCCAGGCA TACCTCATTC TGATTTGGTA
    AGGACTACTG AAAATCAAGT AGACCTCAAG ACGGACGTTT CTCAACCCTC AGATATCAAC
    CATGAACTGT GGATAGCGGC TGGGGCCTGT CATCAGATAC CCCCTCTCCA CCAAAGCAGC
    AAGGATTCTT GTAAAAATGA TGTGAACATC TCTCAGACTG CATACCCACC CCTCCATGAC
    TCAAATCCAC ACAGCCCTCG GCTGCAGACG TTTATAGCAG AAGAAAAGCT AAATCTACAG
    AGAGTGACCA GACAAGACGG GGAAACTGGA AATGGAGCAG AAGAAAATAT TGTTTACCTC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa228084
    Clone ID Related Accession (Same CDS sequence) XM_024981400.1
    Accession Version XM_024981400.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5244bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product rho guanine nucleotide exchange factor 28 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGACGGTCT GCGCGAAGTT TGGCAAAAAT GTTTATCTTC CTAAAGATGC TGAGTTTTAC 
    TTTATTTATA ATGGATCTCA TCAGAGACAC ATCGTGATTG CAGAGCGCAC AGAAGATAAT
    GTTCTCCAGT CCAGCATTCC AGGCCACGGG CTTCAGGAGA CGGTCACCGT GTCTGTGTGC
    CTGTGCTCGG AAGGTTACTC CCCGGTGACC ATGGGCTCTG ACTCCGTGAC CTACGTGGAC
    AACATGGCCT GCAGGCTGGC CCGTCTGCTG GTCACCCAGG CCGATCGCCT CACCGCCAGC
    AGCCACCAGA CCTTGCTGAC CCCGTTTGCC CTGACGGCGG GGGCATTGCC TGCTCTGGAC
    GAGGAGCTCG TGCTGGCCCT GACTCATCTG GAGTTGCCTC TGGGGTGGAC CGTCTTGGGG
    AATTCTTCAC TGGAAGTTTC TCTGCATAGG GAGTCTCTTC TGCACCTGGC TATGAGATGG
    GGCCTTGCTA AGCTTTCCCA GCTCCTTCTG TGTCTCCCTG GGGGAGCCCA GGCCCTGACT
    TTACCCAACG AGGAGGGTGC CACGCCATTA GACTTGGCTT TACATGGTGG ACATTCCAAG
    CTGGTTGAAG ACATCACAAA CTTCCAGGGC AGGCGTTCCC CAGGTTTCTC CTGCGTGCAG
    CTCAGCGAAG ATGCCTCTCT GCGGTATATT CACTCATCAG AAACTCTGAC CCTGACGCTT
    AACCACACAG CCGAGCATTT GCTGGAGGCG GATATTAAAC TCTTCAGAAA ATACTTTTGG
    GATAGAGCCT TTCTCATCAA GGCTCTTGAA GAGCAAGAAG GCACGTCAGA GGAGAAACCA
    GATATGCCCT CCAGCGCTGC AGAAACTGAA GAAGGTATGC ATGCTCCTTC CCCACCTGGG
    GAACTCAGAA AGTGCTCAGC ATTCTCTAGT CAATTGCGAG TTGAAGTGTG TGGAGAAGGA
    GAGCAGGAGC CGATGGACTC GGAGGCGAGA GTAGGTTGCT CTGGGGCCGC AGGGCCCAGA
    GTTCCTTCAG CAGCACTGAC CAAAGAGATT AAGAATTCGG GGTGCAGCAG ATCACCACCC
    GGGAAGGAGG GTGTAAAGCG TGTGAAAAGC CTGGTGGTTC CACTGAATGA ACATGAGGAC
    CAGGAGAGGC TAGATTTGGA TCGTTCCTTT GATGCCCTCA AAAAATCCAA GCACCCCTCA
    ACATTGCTTG CTGCTGGCCA GCTCTCTGAC ATGCTGAATG GTGGTGATGA CGTCTACGCT
    AACTGCATGG TGATAGACCA GGTTGGTGAT TTGGACATTA ACTATATTAA TATAGAGGAA
    ATCCCTGCAA ATACCTGTCT TGAATCCATG GATTCTTCTC TGGGGCCTGA GAGCAGCAAA
    CCCAGCCTTC CTACTGAAGC CAGTGCTGGC ACATACCCAC CAAATAAGAG GCCAGAAGTG
    ACGTCACACA CGGAAGCCCA ATCATCTTTC ATATCATCTA GTTCGTATGC TTCCAATTTG
    AATCTTTCTT TTGGTCTTCG TGGATTTGAA AAGGAGCAAA GTCGTCTAAA GAAAAGAAGT
    TCCAGCCTTG ATGCCTTGGA TGCTGACAGT GAAGGAGAGG GCCATTCGGA GCAGTCGCAC
    AATTGCTACA CTCCCGTGTG TCAGAGTTCC TCGAGAACTG GCATTGCTAG CGGGGATGAA
    TTGGACTCTT TTGAGACCAA CACTGAACCA GATTTTAATA TCTCCAGGAC GGAATCATTT
    TCTTTATCAA GTAGCCTGCA GCTGAAGGAA TCATTGTTTT CTGGAATCCG TTCACGTTCG
    TATTCCTGCT CATCACCCAA AATTTCTTTA GGAAAAACTC GATTGATGCG TGATTTTACA
    GTGTGTAATT CAATTGAAGA GCAAAGAGCT TACAGCTTGC CGGAGCCACC GAGAGAACAA
    AGAATTCAGG AAGAAGAATG GGATAAATAT ATCATACCTG CCAAATCAGA ATCTGAAAAA
    TACAAAGTAA GCCGAACTTT TAGCTTCCTC ATGAATAGGA TGACCAGCCC TCGGAATAAA
    TCAAAGACAA AAGGCAAGGA TGCCAAAGAC AAAGAGAAAC TGACTCGACA TCAGTTTGTC
    CCTGGAACTT TCTCTGGGGT TCTGCAGTGT TTGGTTTGCG ATAAAACGCT CCTGGGGAAA
    GAGTCATTCC AGTGCTCCAA CTGTAATGGA AACGTACATA AAGTCTGTAA AGATGCTGCC
    CCTCCGTGTA CCAAGAAATT CCAAGACAAA TATAATAAGA ACAAACCACA GACCATCCTT
    GGAAATTCTT CGTTTCGAGA CGTCCCATTG CCTGGGCTCT CTCTGCACCC TTCCTCCTCC
    ATGCCTATCG GGTTGCCAAC TGGGAGGAAG GAGATGGCAG GGCAAGTGCA TCCCTTGTCC
    AGAAGTGTCC CAGGCACCAC CTTGGAAAGC TTCAGGAGGC CGGGGGCATC CTTGGAGTCC
    GAGAATGATG GCAGCACCTG GAGAAGCAGG TCTCACTCTG ACGAGCTGTT CCAGCCCATG
    GGCTCGTCCC CCTCCACGGA CTCCTTCTTA ACGGAAGATG TCGTGGATTC TTCTCTGTGG
    AGTGACCTCA GCGGCGATGC CCAGGAGTTT GAAGCGGAAT CTTGGAGTCT GGTGGTGGAC
    CCTTCATTTT GTAGTCGGCA GGAGAAGGAT GTCATCCAAA GACAGGATGT CATTTTTGAG
    CTAATGCAGA CAGAACTGCA TCACATCCAG ACCCTGTTCA TCATGTCTGA GATCTTCAGG
    AAAGGGATGA AAGAGGAGCT GCAGCTGGAC CACAGCACTG TGGACAAAAT CTTCCCCTGT
    CTGGACGAGT TACTTGAAAT CCACAAGCAT TTCTTCTGTA GTATGAAGGA GCGAAGGCAG
    GAATCCTGTG TTGGCAATGA CAGGAATTTC ATCATCGGCC GAATTGGAGA TATTCTAGTA
    CAACAGTTTT CAGAAGAAAA TGCAAATAAA ATGAAGAAAA TATATGGGGA ATTCTGTAGC
    CATCACAAAG AGGCTGTTAG CCTCTTTAAA GAACTCCAGC AGAATAAAAA GTTTCAGAAT
    TTTATTAAGC TCCGAAATAG TAACCTTCTG GCTCGACGCC GAGGAATTCC AGAATGCATT
    CTGCTGGTCA CTCAGCGCAT CACAAAATAC CCTGTCTTGG TGGAAAGGAT ATTGCAGTAC
    ACAAAGGAGA GCACTGAAGA ACAGAGAGAC TTATGCAAAG CTCTTTGCTT AATTAAAGAC
    ATGATTGCAG CTGTGGATTT AAAAGTCAGC GAATATGAGA AAAAACAAAA ATGGCTTGAG
    ATCCTAAATA AGATCGAAAA CAAAACATAC ACCAAGCTCA AAAATGGCCA TGTGTTTAGG
    AAACAGGCAC TGATGAGTAA AGAGAGGACC CTGTTATATG ATGGCCTTGT CTATTGGAAA
    ACTGCTACAG GTCGTTTCAA AGATATCCTA GCTCTACTTC TAACTGATGT GCTGCTCTTT
    TTACAAGAAA AAGACCAGAA ATACATCTTT GCAGCTGTTG ATCAGAAGCC ATCAGTTATT
    TCCCTTCAGA AGCTCATTGC TAGGGAAGTT GCGAATGAGG AGAGAGGAAT GTTTTTGATC
    AGCGCTTCAT CTGCTGGTCC CGAGATGTAT GAAATCCATA CCAACTCCAA GGAGGAAAGA
    AATACCTGGA TGAGACGGAT CCAACAGGCA GTAGAAAGTT GCCCAGAAGA AGAAGGGGGA
    AGGACAAGTG AGTCTGATGA AGAGAGGAGG AAAGCTGAAG CCAGAGTGGC GAAGATTCAG
    CAATGCCAAG AAATACTCAG TAACCAAGAT CAACAAATTT GCACATATTT GGAAGAGAAA
    CTGCATATCT ACGCGAAACT TGGAGAACTA AGTGGATTTC AGGATGTCCA TCTAGAGCCC
    CACCTCCTCA TTCAACCAGA CCCCGGAGAG CCTCCCCAGG CAGCCTCATT ACTGGCCGCA
    GCACTGAAAG AGGCTGAGAG TCTGCAGGTG GCAGTGAAGG CTTCCCAGAT GAGCGATGCA
    TGCCAGTCCC CCGAGGAAGG TTGTGGAGAA CCTGCTTTGC CGGACGTGCT CCATTCTCAG
    GACGCACCAG GATCGTCTAC TGCTTCACTA GTCACAGAAG GGACAGGAGG AAGAGGCTGT
    TGGGATGTGG ATGCCGGGAC CCAGGGTGTG GGAACCGACC TGGCGGTTTC TGATGCGGGG
    GAGAAGGTGG AATATAGAAG TTTCCCAGGT TCTTTGCAGT CAGAGATTAT ACAAGCGATA
    CAGAATTTAA CTCGTCTCTT ATATAGCCTT CAGGCCGCCT TGACCATTCA GGATAGCCAC
    ATTGAGATCC ACAGGCTGGT TCTCCAACAG CGGGAGAGCC TGGCCCCGGG CCCCGCGTTC
    CGAGGCAGCC CCTTTCAGGA CCAGGAGAAG TCCCGCAGCC TGGAGAAGCA GCGGGAGGAG
    CTGGCCGATA CCCACAAGCT GCAGCTCCAG TTCCAGCAAG ACCAGCACCG CTGGCTCCGC
    AGGTGCGACC AGCAGCAGCG CGAGCAGGAG GCCCGGGCCA GCCGGCTGCA GGAGCGCGAG
    AGGGAGTGCC GGGCGCAGGA GGAGCTGCTG CTCAGGAGCC GGGGCGAGCT GGACCAGCAG
    CTCCAAGACT ACCAGCAGAG CCTGGAGCGC CTGCGCGAGG GCCAGCGCCT GGTGGAGAGG
    GAGCGGGAGA GGATGCGGGC CCAGCAGAGC CTGCTGCGCC ACTGGAAGCA CGGGCGGCAG
    AGCAGCCTTC CCGCCGCGTT TTCTCCAGGA GCCCTGGAGG TAATGGAACT TAATCGATCT
    GAGACTTTAC GTCATGAAAA TTCATTCTTC ATCAATGAAG CTTTAGTGCA AATGTCACTC
    AACCCTTTGA ACAAACCGCC GCCACCAGGT ATCCATCAGG ATGCGGCTTA CCCCCCAGGC
    ATACCTCATT CTGATTTGGT AAGGACTACT GAAAATCAAG TAGACCTCAA GACGGACGTT
    TCTCAACCCT CAGATATCAA CCATGAACTG TGGATAGCGG CTGGGGCCTG TCATCAGATA
    CCCCCTCTCC ACCAAAGCAG CAAGGATTCT TGTAAAAATG ATGTGAACAT CTCTCAGACT
    GCATACCCAC CCCTCCATGA CTCAAATCCA CACAGCCCTC GGCTGCAGAC GTTTATAGCA
    GAAGAAAAGC TAAATCTACA GAGAGTGACC AGACAAGACG GGGAAACTGG AAATGGAGCA
    GAAGAAAATA TTGTTTACCT CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024837168.1
    CDS1573..6816
    Translation

    Target ORF information:

    RefSeq Version XM_024981400.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981400.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGACGGTCT GCGCGAAGTT TGGCAAAAAT GTTTATCTTC CTAAAGATGC TGAGTTTTAC 
    TTTATTTATA ATGGATCTCA TCAGAGACAC ATCGTGATTG CAGAGCGCAC AGAAGATAAT
    GTTCTCCAGT CCAGCATTCC AGGCCACGGG CTTCAGGAGA CGGTCACCGT GTCTGTGTGC
    CTGTGCTCGG AAGGTTACTC CCCGGTGACC ATGGGCTCTG ACTCCGTGAC CTACGTGGAC
    AACATGGCCT GCAGGCTGGC CCGTCTGCTG GTCACCCAGG CCGATCGCCT CACCGCCAGC
    AGCCACCAGA CCTTGCTGAC CCCGTTTGCC CTGACGGCGG GGGCATTGCC TGCTCTGGAC
    GAGGAGCTCG TGCTGGCCCT GACTCATCTG GAGTTGCCTC TGGGGTGGAC CGTCTTGGGG
    AATTCTTCAC TGGAAGTTTC TCTGCATAGG GAGTCTCTTC TGCACCTGGC TATGAGATGG
    GGCCTTGCTA AGCTTTCCCA GCTCCTTCTG TGTCTCCCTG GGGGAGCCCA GGCCCTGACT
    TTACCCAACG AGGAGGGTGC CACGCCATTA GACTTGGCTT TACATGGTGG ACATTCCAAG
    CTGGTTGAAG ACATCACAAA CTTCCAGGGC AGGCGTTCCC CAGGTTTCTC CTGCGTGCAG
    CTCAGCGAAG ATGCCTCTCT GCGGTATATT CACTCATCAG AAACTCTGAC CCTGACGCTT
    AACCACACAG CCGAGCATTT GCTGGAGGCG GATATTAAAC TCTTCAGAAA ATACTTTTGG
    GATAGAGCCT TTCTCATCAA GGCTCTTGAA GAGCAAGAAG GCACGTCAGA GGAGAAACCA
    GATATGCCCT CCAGCGCTGC AGAAACTGAA GAAGGTATGC ATGCTCCTTC CCCACCTGGG
    GAACTCAGAA AGTGCTCAGC ATTCTCTAGT CAATTGCGAG TTGAAGTGTG TGGAGAAGGA
    GAGCAGGAGC CGATGGACTC GGAGGCGAGA GTAGGTTGCT CTGGGGCCGC AGGGCCCAGA
    GTTCCTTCAG CAGCACTGAC CAAAGAGATT AAGAATTCGG GGTGCAGCAG ATCACCACCC
    GGGAAGGAGG GTGTAAAGCG TGTGAAAAGC CTGGTGGTTC CACTGAATGA ACATGAGGAC
    CAGGAGAGGC TAGATTTGGA TCGTTCCTTT GATGCCCTCA AAAAATCCAA GCACCCCTCA
    ACATTGCTTG CTGCTGGCCA GCTCTCTGAC ATGCTGAATG GTGGTGATGA CGTCTACGCT
    AACTGCATGG TGATAGACCA GGTTGGTGAT TTGGACATTA ACTATATTAA TATAGAGGAA
    ATCCCTGCAA ATACCTGTCT TGAATCCATG GATTCTTCTC TGGGGCCTGA GAGCAGCAAA
    CCCAGCCTTC CTACTGAAGC CAGTGCTGGC ACATACCCAC CAAATAAGAG GCCAGAAGTG
    ACGTCACACA CGGAAGCCCA ATCATCTTTC ATATCATCTA GTTCGTATGC TTCCAATTTG
    AATCTTTCTT TTGGTCTTCG TGGATTTGAA AAGGAGCAAA GTCGTCTAAA GAAAAGAAGT
    TCCAGCCTTG ATGCCTTGGA TGCTGACAGT GAAGGAGAGG GCCATTCGGA GCAGTCGCAC
    AATTGCTACA CTCCCGTGTG TCAGAGTTCC TCGAGAACTG GCATTGCTAG CGGGGATGAA
    TTGGACTCTT TTGAGACCAA CACTGAACCA GATTTTAATA TCTCCAGGAC GGAATCATTT
    TCTTTATCAA GTAGCCTGCA GCTGAAGGAA TCATTGTTTT CTGGAATCCG TTCACGTTCG
    TATTCCTGCT CATCACCCAA AATTTCTTTA GGAAAAACTC GATTGATGCG TGATTTTACA
    GTGTGTAATT CAATTGAAGA GCAAAGAGCT TACAGCTTGC CGGAGCCACC GAGAGAACAA
    AGAATTCAGG AAGAAGAATG GGATAAATAT ATCATACCTG CCAAATCAGA ATCTGAAAAA
    TACAAAGTAA GCCGAACTTT TAGCTTCCTC ATGAATAGGA TGACCAGCCC TCGGAATAAA
    TCAAAGACAA AAGGCAAGGA TGCCAAAGAC AAAGAGAAAC TGACTCGACA TCAGTTTGTC
    CCTGGAACTT TCTCTGGGGT TCTGCAGTGT TTGGTTTGCG ATAAAACGCT CCTGGGGAAA
    GAGTCATTCC AGTGCTCCAA CTGTAATGGA AACGTACATA AAGTCTGTAA AGATGCTGCC
    CCTCCGTGTA CCAAGAAATT CCAAGACAAA TATAATAAGA ACAAACCACA GACCATCCTT
    GGAAATTCTT CGTTTCGAGA CGTCCCATTG CCTGGGCTCT CTCTGCACCC TTCCTCCTCC
    ATGCCTATCG GGTTGCCAAC TGGGAGGAAG GAGATGGCAG GGCAAGTGCA TCCCTTGTCC
    AGAAGTGTCC CAGGCACCAC CTTGGAAAGC TTCAGGAGGC CGGGGGCATC CTTGGAGTCC
    GAGAATGATG GCAGCACCTG GAGAAGCAGG TCTCACTCTG ACGAGCTGTT CCAGCCCATG
    GGCTCGTCCC CCTCCACGGA CTCCTTCTTA ACGGAAGATG TCGTGGATTC TTCTCTGTGG
    AGTGACCTCA GCGGCGATGC CCAGGAGTTT GAAGCGGAAT CTTGGAGTCT GGTGGTGGAC
    CCTTCATTTT GTAGTCGGCA GGAGAAGGAT GTCATCCAAA GACAGGATGT CATTTTTGAG
    CTAATGCAGA CAGAACTGCA TCACATCCAG ACCCTGTTCA TCATGTCTGA GATCTTCAGG
    AAAGGGATGA AAGAGGAGCT GCAGCTGGAC CACAGCACTG TGGACAAAAT CTTCCCCTGT
    CTGGACGAGT TACTTGAAAT CCACAAGCAT TTCTTCTGTA GTATGAAGGA GCGAAGGCAG
    GAATCCTGTG TTGGCAATGA CAGGAATTTC ATCATCGGCC GAATTGGAGA TATTCTAGTA
    CAACAGTTTT CAGAAGAAAA TGCAAATAAA ATGAAGAAAA TATATGGGGA ATTCTGTAGC
    CATCACAAAG AGGCTGTTAG CCTCTTTAAA GAACTCCAGC AGAATAAAAA GTTTCAGAAT
    TTTATTAAGC TCCGAAATAG TAACCTTCTG GCTCGACGCC GAGGAATTCC AGAATGCATT
    CTGCTGGTCA CTCAGCGCAT CACAAAATAC CCTGTCTTGG TGGAAAGGAT ATTGCAGTAC
    ACAAAGGAGA GCACTGAAGA ACAGAGAGAC TTATGCAAAG CTCTTTGCTT AATTAAAGAC
    ATGATTGCAG CTGTGGATTT AAAAGTCAGC GAATATGAGA AAAAACAAAA ATGGCTTGAG
    ATCCTAAATA AGATCGAAAA CAAAACATAC ACCAAGCTCA AAAATGGCCA TGTGTTTAGG
    AAACAGGCAC TGATGAGTAA AGAGAGGACC CTGTTATATG ATGGCCTTGT CTATTGGAAA
    ACTGCTACAG GTCGTTTCAA AGATATCCTA GCTCTACTTC TAACTGATGT GCTGCTCTTT
    TTACAAGAAA AAGACCAGAA ATACATCTTT GCAGCTGTTG ATCAGAAGCC ATCAGTTATT
    TCCCTTCAGA AGCTCATTGC TAGGGAAGTT GCGAATGAGG AGAGAGGAAT GTTTTTGATC
    AGCGCTTCAT CTGCTGGTCC CGAGATGTAT GAAATCCATA CCAACTCCAA GGAGGAAAGA
    AATACCTGGA TGAGACGGAT CCAACAGGCA GTAGAAAGTT GCCCAGAAGA AGAAGGGGGA
    AGGACAAGTG AGTCTGATGA AGAGAGGAGG AAAGCTGAAG CCAGAGTGGC GAAGATTCAG
    CAATGCCAAG AAATACTCAG TAACCAAGAT CAACAAATTT GCACATATTT GGAAGAGAAA
    CTGCATATCT ACGCGAAACT TGGAGAACTA AGTGGATTTC AGGATGTCCA TCTAGAGCCC
    CACCTCCTCA TTCAACCAGA CCCCGGAGAG CCTCCCCAGG CAGCCTCATT ACTGGCCGCA
    GCACTGAAAG AGGCTGAGAG TCTGCAGGTG GCAGTGAAGG CTTCCCAGAT GAGCGATGCA
    TGCCAGTCCC CCGAGGAAGG TTGTGGAGAA CCTGCTTTGC CGGACGTGCT CCATTCTCAG
    GACGCACCAG GATCGTCTAC TGCTTCACTA GTCACAGAAG GGACAGGAGG AAGAGGCTGT
    TGGGATGTGG ATGCCGGGAC CCAGGGTGTG GGAACCGACC TGGCGGTTTC TGATGCGGGG
    GAGAAGGTGG AATATAGAAG TTTCCCAGGT TCTTTGCAGT CAGAGATTAT ACAAGCGATA
    CAGAATTTAA CTCGTCTCTT ATATAGCCTT CAGGCCGCCT TGACCATTCA GGATAGCCAC
    ATTGAGATCC ACAGGCTGGT TCTCCAACAG CGGGAGAGCC TGGCCCCGGG CCCCGCGTTC
    CGAGGCAGCC CCTTTCAGGA CCAGGAGAAG TCCCGCAGCC TGGAGAAGCA GCGGGAGGAG
    CTGGCCGATA CCCACAAGCT GCAGCTCCAG TTCCAGCAAG ACCAGCACCG CTGGCTCCGC
    AGGTGCGACC AGCAGCAGCG CGAGCAGGAG GCCCGGGCCA GCCGGCTGCA GGAGCGCGAG
    AGGGAGTGCC GGGCGCAGGA GGAGCTGCTG CTCAGGAGCC GGGGCGAGCT GGACCAGCAG
    CTCCAAGACT ACCAGCAGAG CCTGGAGCGC CTGCGCGAGG GCCAGCGCCT GGTGGAGAGG
    GAGCGGGAGA GGATGCGGGC CCAGCAGAGC CTGCTGCGCC ACTGGAAGCA CGGGCGGCAG
    AGCAGCCTTC CCGCCGCGTT TTCTCCAGGA GCCCTGGAGG TAATGGAACT TAATCGATCT
    GAGACTTTAC GTCATGAAAA TTCATTCTTC ATCAATGAAG CTTTAGTGCA AATGTCACTC
    AACCCTTTGA ACAAACCGCC GCCACCAGGT ATCCATCAGG ATGCGGCTTA CCCCCCAGGC
    ATACCTCATT CTGATTTGGT AAGGACTACT GAAAATCAAG TAGACCTCAA GACGGACGTT
    TCTCAACCCT CAGATATCAA CCATGAACTG TGGATAGCGG CTGGGGCCTG TCATCAGATA
    CCCCCTCTCC ACCAAAGCAG CAAGGATTCT TGTAAAAATG ATGTGAACAT CTCTCAGACT
    GCATACCCAC CCCTCCATGA CTCAAATCCA CACAGCCCTC GGCTGCAGAC GTTTATAGCA
    GAAGAAAAGC TAAATCTACA GAGAGTGACC AGACAAGACG GGGAAACTGG AAATGGAGCA
    GAAGAAAATA TTGTTTACCT CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa228085
    Clone ID Related Accession (Same CDS sequence) XM_024981401.1
    Accession Version XM_024981401.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5235bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product rho guanine nucleotide exchange factor 28 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGCAGCCCA CTCTTCACTT TCTTCCCATC TTTAGGTGTC CGTTCAGCTG TGAGCTCTGT 
    GAGGAGTGGA TCGTTTCCTG GTCATCTTTG TGCAATGCTG AGCCCATTGA AGATGCGGAA
    ACCATGGAGC TGAACTGCAG CGAAGTACCT CTGTATGGGC AGATGACGGT CTGCGCGAAG
    TTTGGCAAAA ATGTTTATCT TCCTAAAGAT GCTGAGTTTT ACTTTATTTA TAATGGATCT
    CATCAGAGAC ACATCGTGAT TGCAGAGCGC ACAGAAGATA ATGTTCTCCA GTCCAGCATT
    CCAGGCCACG GGCTTCAGGA GACGGTCACC GTGTCTGTGT GCCTGTGCTC GGAAGGTTAC
    TCCCCGGTGA CCATGGGCTC TGACTCCGTG ACCTACGTGG ACAACATGGC CTGCAGGCTG
    GCCCGTCTGC TGGTCACCCA GGCCGATCGC CTCACCGCCA GCAGCCACCA GACCTTGCTG
    ACCCCGTTTG CCCTGACGGC GGGGGCATTG CCTGCTCTGG ACGAGGAGCT CGTGCTGGCC
    CTGACTCATC TGGAGTTGCC TCTGGGGTGG ACCGTCTTGG GGAATTCTTC ACTGGAAGTT
    TCTCTGCATA GGGAGTCTCT TCTGCACCTG GCTATGAGAT GGGGCCTTGC TAAGCTTTCC
    CAGCTCCTTC TGTGTCTCCC TGGGGGAGCC CAGGCCCTGA CTTTACCCAA CGAGGAGGGT
    GCCACGCCAT TAGACTTGGC TTTACATGGT GGACATTCCA AGCTGGTTGA AGACATCACA
    AACTTCCAGG GCAGGCGTTC CCCAGGTTTC TCCTGCGTGC AGCTCAGCGA AGATGCCTCT
    CTGCGGTATA TTCACTCATC AGAAACTCTG ACCCTGACGC TTAACCACAC AGCCGAGCAT
    TTGCTGGAGG CGGATATTAA ACTCTTCAGA AAATACTTTT GGGATAGAGC CTTTCTCATC
    AAGGCTCTTG AAGAGCAAGA AGGCACGTCA GAGGAGAAAC CAGATATGCC CTCCAGCGCT
    GCAGAAACTG AAGAAGAGAT TAAGAATTCG GGGTGCAGCA GATCACCACC CGGGAAGGAG
    GGTGTAAAGC GTGTGAAAAG CCTGGTGGTT CCACTGAATG AACATGAGGA CCAGGAGAGG
    CTAGATTTGG ATCGTTCCTT TGATGCCCTC AAAAAATCCA AGCACCCCTC AACATTGCTT
    GCTGCTGGCC AGCTCTCTGA CATGCTGAAT GGTGGTGATG ACGTCTACGC TAACTGCATG
    GTGATAGACC AGGTTGGTGA TTTGGACATT AACTATATTA ATATAGAGGA AATCCCTGCA
    AATACCTGTC TTGAATCCAT GGATTCTTCT CTGGGGCCTG AGAGCAGCAA ACCCAGCCTT
    CCTACTGAAG CCAGTGCTGG CACATACCCA CCAAATAAGA GGCCAGAAGT GACGTCACAC
    ACGGAAGCCC AATCATCTTT CATATCATCT AGTTCGTATG CTTCCAATTT GAATCTTTCT
    TTTGGTCTTC GTGGATTTGA AAAGGAGCAA AGTCGTCTAA AGAAAAGAAG TTCCAGCCTT
    GATGCCTTGG ATGCTGACAG TGAAGGAGAG GGCCATTCGG AGCAGTCGCA CAATTGCTAC
    ACTCCCGTGT GTCAGAGTTC CTCGAGAACT GGCATTGCTA GCGGGGATGA ATTGGACTCT
    TTTGAGACCA ACACTGAACC AGATTTTAAT ATCTCCAGGA CGGAATCATT TTCTTTATCA
    AGTAGCCTGC AGCTGAAGGA ATCATTGTTT TCTGGAATCC GTTCACGTTC GTATTCCTGC
    TCATCACCCA AAATTTCTTT AGGAAAAACT CGATTGATGC GTGATTTTAC AGTGTGTAAT
    TCAATTGAAG AGCAAAGAGC TTACAGCTTG CCGGAGCCAC CGAGAGAACA AAGAATTCAG
    GAAGAAGAAT GGGATAAATA TATCATACCT GCCAAATCAG AATCTGAAAA ATACAAAGTA
    AGCCGAACTT TTAGCTTCCT CATGAATAGG ATGACCAGCC CTCGGAATAA ATCAAAGACA
    AAAGGCAAGG ATGCCAAAGA CAAAGAGAAA CTGACTCGAC ATCAGTTTGT CCCTGGAACT
    TTCTCTGGGG TTCTGCAGTG TTTGGTTTGC GATAAAACGC TCCTGGGGAA AGAGTCATTC
    CAGTGCTCCA ACTGTAATGG AAACGTACAT AAAGTCTGTA AAGATGCTGC CCCTCCGTGT
    ACCAAGAAAT TCCAAGACAA ATATAATAAG AACAAACCAC AGACCATCCT TGGAAATTCT
    TCGTTTCGAG ACGTCCCATT GCCTGGGCTC TCTCTGCACC CTTCCTCCTC CATGCCTATC
    GGGTTGCCAA CTGGGAGGAA GGAGATGGCA GGGCAAGTGC ATCCCTTGTC CAGAAGTGTC
    CCAGGCACCA CCTTGGAAAG CTTCAGGAGG CCGGGGGCAT CCTTGGAGTC CGAGAATGAT
    GGCAGCACCT GGAGAAGCAG GTCTCACTCT GACGAGCTGT TCCAGCCCAT GGGCTCGTCC
    CCCTCCACGG ACTCCTTCTT AACGGAAGAT GTCGTGGATT CTTCTCTGTG GAGTGACCTC
    AGCGGCGATG CCCAGGAGTT TGAAGCGGAA TCTTGGAGTC TGGTGGTGGA CCCTTCATTT
    TGTAGTCGGC AGGAGAAGGA TGTCATCCAA AGACAGGATG TCATTTTTGA GCTAATGCAG
    ACAGAACTGC ATCACATCCA GACCCTGTTC ATCATGTCTG AGATCTTCAG GAAAGGGATG
    AAAGAGGAGC TGCAGCTGGA CCACAGCACT GTGGACAAAA TCTTCCCCTG TCTGGACGAG
    TTACTTGAAA TCCACAAGCA TTTCTTCTGT AGTATGAAGG AGCGAAGGCA GGAATCCTGT
    GTTGGCAATG ACAGGAATTT CATCATCGGC CGAATTGGAG ATATTCTAGT ACAACAGTTT
    TCAGAAGAAA ATGCAAATAA AATGAAGAAA ATATATGGGG AATTCTGTAG CCATCACAAA
    GAGGCTGTTA GCCTCTTTAA AGAACTCCAG CAGAATAAAA AGTTTCAGAA TTTTATTAAG
    CTCCGAAATA GTAACCTTCT GGCTCGACGC CGAGGAATTC CAGAATGCAT TCTGCTGGTC
    ACTCAGCGCA TCACAAAATA CCCTGTCTTG GTGGAAAGGA TATTGCAGTA CACAAAGGAG
    AGCACTGAAG AACAGAGAGA CTTATGCAAA GCTCTTTGCT TAATTAAAGA CATGATTGCA
    GCTGTGGATT TAAAAGTCAG CGAATATGAG AAAAAACAAA AATGGCTTGA GATCCTAAAT
    AAGATCGAAA ACAAAACATA CACCAAGCTC AAAAATGGCC ATGTGTTTAG GAAACAGGCA
    CTGATGAGTA AAGAGAGGAC CCTGTTATAT GATGGCCTTG TCTATTGGAA AACTGCTACA
    GGTCGTTTCA AAGATATCCT AGCTCTACTT CTAACTGATG TGCTGCTCTT TTTACAAGAA
    AAAGACCAGA AATACATCTT TGCAGCTGTT GATCAGAAGC CATCAGTTAT TTCCCTTCAG
    AAGCTCATTG CTAGGGAAGT TGCGAATGAG GAGAGAGGAA TGTTTTTGAT CAGCGCTTCA
    TCTGCTGGTC CCGAGATGTA TGAAATCCAT ACCAACTCCA AGGAGGAAAG AAATACCTGG
    ATGAGACGGA TCCAACAGGC AGTAGAAAGT TGCCCAGAAG AAGAAGGGGG AAGGACAAGT
    GAGTCTGATG AAGAGAGGAG GAAAGCTGAA GCCAGAGTGG CGAAGATTCA GCAATGCCAA
    GAAATACTCA GTAACCAAGA TCAACAAATT TGCACATATT TGGAAGAGAA ACTGCATATC
    TACGCGAAAC TTGGAGAACT AAGTGGATTT CAGGATGTCC ATCTAGAGCC CCACCTCCTC
    ATTCAACCAG ACCCCGGAGA GCCTCCCCAG GCAGCCTCAT TACTGGCCGC AGCACTGAAA
    GAGGCTGAGA GTCTGCAGGT GGCAGTGAAG GCTTCCCAGA TGAGCGATGC ATGCCAGTCC
    CCCGAGGAAG GTTGTGGAGA ACCTGCTTTG CCGGACGTGC TCCATTCTCA GGACGCACCA
    GGATCGTCTA CTGCTTCACT AGTCACAGAA GGGACAGGAG GAAGAGGCTG TTGGGATGTG
    GATGCCGGGA CCCAGGGTGT GGGAACCGAC CTGGCGGTTT CTGATGCGGG GGAGAAGGTG
    GAATATAGAA GTTTCCCAGG TTCTTTGCAG TCAGAGATTA TACAAGCGAT ACAGAATTTA
    ACTCGTCTCT TATATAGCCT TCAGGCCGCC TTGACCATTC AGGATAGCCA CATTGAGATC
    CACAGGCTGG TTCTCCAACA GCGGGAGAGC CTGGCCCCGG GCCCCGCGTT CCGAGGCAGC
    CCCTTTCAGG ACCAGGAGAA GTCCCGCAGC CTGGAGAAGC AGCGGGAGGA GCTGGCCGAT
    ACCCACAAGC TGCAGCTCCA GTTCCAGCAA GACCAGCACC GCTGGCTCCG CAGGTGCGAC
    CAGCAGCAGC GCGAGCAGGA GGCCCGGGCC AGCCGGCTGC AGGAGCGCGA GAGGGAGTGC
    CGGGCGCAGG AGGAGCTGCT GCTCAGGAGC CGGGGCGAGC TGGACCAGCA GCTCCAAGAC
    TACCAGCAGA GCCTGGAGCG CCTGCGCGAG GGCCAGCGCC TGGTGGAGAG GGAGCGGGAG
    AGGATGCGGG CCCAGCAGAG CCTGCTGCGC CACTGGAAGC ACGGGCGGCA GAGCAGCCTT
    CCCGCCGCGT TTTCTCCAGG AGCCCTGGAG GTAATGGAAC TTAATCGATC TGAGACTTTA
    CGTCATGAAA ATTCATTCTT CATCAATGAA GCTTTAGTGC AAATGTCACT CAACCCTTTG
    AACAAACCGC CGCCACCAGG TATCCATCAG GATGCGGCTT ACCCCCCAGG CATACCTCAT
    TCTGATTTGG TAAGGACTAC TGAAAATCAA GTAGACCTCA AGACGGACGT TTCTCAACCC
    TCAGATATCA ACCATGAACT GTGGATAGCG GCTGGGGCCT GTCATCAGAT ACCCCCTCTC
    CACCAAAGCA GCAAGGATTC TTGTAAAAAT GATGTGAACA TCTCTCAGAC TGCATACCCA
    CCCCTCCATG ACTCAAATCC ACACAGCCCT CGGCTGCAGA CGTTTATAGC AGAAGAAAAG
    CTAAATCTAC AGAGAGTGAC CAGACAAGAC GGGGAAACTG GAAATGGAGC AGAAGAAAAT
    ATTGTTTACC TCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024837169.1
    CDS17..5251
    Translation

    Target ORF information:

    RefSeq Version XM_024981401.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981401.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGCAGCCCA CTCTTCACTT TCTTCCCATC TTTAGGTGTC CGTTCAGCTG TGAGCTCTGT 
    GAGGAGTGGA TCGTTTCCTG GTCATCTTTG TGCAATGCTG AGCCCATTGA AGATGCGGAA
    ACCATGGAGC TGAACTGCAG CGAAGTACCT CTGTATGGGC AGATGACGGT CTGCGCGAAG
    TTTGGCAAAA ATGTTTATCT TCCTAAAGAT GCTGAGTTTT ACTTTATTTA TAATGGATCT
    CATCAGAGAC ACATCGTGAT TGCAGAGCGC ACAGAAGATA ATGTTCTCCA GTCCAGCATT
    CCAGGCCACG GGCTTCAGGA GACGGTCACC GTGTCTGTGT GCCTGTGCTC GGAAGGTTAC
    TCCCCGGTGA CCATGGGCTC TGACTCCGTG ACCTACGTGG ACAACATGGC CTGCAGGCTG
    GCCCGTCTGC TGGTCACCCA GGCCGATCGC CTCACCGCCA GCAGCCACCA GACCTTGCTG
    ACCCCGTTTG CCCTGACGGC GGGGGCATTG CCTGCTCTGG ACGAGGAGCT CGTGCTGGCC
    CTGACTCATC TGGAGTTGCC TCTGGGGTGG ACCGTCTTGG GGAATTCTTC ACTGGAAGTT
    TCTCTGCATA GGGAGTCTCT TCTGCACCTG GCTATGAGAT GGGGCCTTGC TAAGCTTTCC
    CAGCTCCTTC TGTGTCTCCC TGGGGGAGCC CAGGCCCTGA CTTTACCCAA CGAGGAGGGT
    GCCACGCCAT TAGACTTGGC TTTACATGGT GGACATTCCA AGCTGGTTGA AGACATCACA
    AACTTCCAGG GCAGGCGTTC CCCAGGTTTC TCCTGCGTGC AGCTCAGCGA AGATGCCTCT
    CTGCGGTATA TTCACTCATC AGAAACTCTG ACCCTGACGC TTAACCACAC AGCCGAGCAT
    TTGCTGGAGG CGGATATTAA ACTCTTCAGA AAATACTTTT GGGATAGAGC CTTTCTCATC
    AAGGCTCTTG AAGAGCAAGA AGGCACGTCA GAGGAGAAAC CAGATATGCC CTCCAGCGCT
    GCAGAAACTG AAGAAGAGAT TAAGAATTCG GGGTGCAGCA GATCACCACC CGGGAAGGAG
    GGTGTAAAGC GTGTGAAAAG CCTGGTGGTT CCACTGAATG AACATGAGGA CCAGGAGAGG
    CTAGATTTGG ATCGTTCCTT TGATGCCCTC AAAAAATCCA AGCACCCCTC AACATTGCTT
    GCTGCTGGCC AGCTCTCTGA CATGCTGAAT GGTGGTGATG ACGTCTACGC TAACTGCATG
    GTGATAGACC AGGTTGGTGA TTTGGACATT AACTATATTA ATATAGAGGA AATCCCTGCA
    AATACCTGTC TTGAATCCAT GGATTCTTCT CTGGGGCCTG AGAGCAGCAA ACCCAGCCTT
    CCTACTGAAG CCAGTGCTGG CACATACCCA CCAAATAAGA GGCCAGAAGT GACGTCACAC
    ACGGAAGCCC AATCATCTTT CATATCATCT AGTTCGTATG CTTCCAATTT GAATCTTTCT
    TTTGGTCTTC GTGGATTTGA AAAGGAGCAA AGTCGTCTAA AGAAAAGAAG TTCCAGCCTT
    GATGCCTTGG ATGCTGACAG TGAAGGAGAG GGCCATTCGG AGCAGTCGCA CAATTGCTAC
    ACTCCCGTGT GTCAGAGTTC CTCGAGAACT GGCATTGCTA GCGGGGATGA ATTGGACTCT
    TTTGAGACCA ACACTGAACC AGATTTTAAT ATCTCCAGGA CGGAATCATT TTCTTTATCA
    AGTAGCCTGC AGCTGAAGGA ATCATTGTTT TCTGGAATCC GTTCACGTTC GTATTCCTGC
    TCATCACCCA AAATTTCTTT AGGAAAAACT CGATTGATGC GTGATTTTAC AGTGTGTAAT
    TCAATTGAAG AGCAAAGAGC TTACAGCTTG CCGGAGCCAC CGAGAGAACA AAGAATTCAG
    GAAGAAGAAT GGGATAAATA TATCATACCT GCCAAATCAG AATCTGAAAA ATACAAAGTA
    AGCCGAACTT TTAGCTTCCT CATGAATAGG ATGACCAGCC CTCGGAATAA ATCAAAGACA
    AAAGGCAAGG ATGCCAAAGA CAAAGAGAAA CTGACTCGAC ATCAGTTTGT CCCTGGAACT
    TTCTCTGGGG TTCTGCAGTG TTTGGTTTGC GATAAAACGC TCCTGGGGAA AGAGTCATTC
    CAGTGCTCCA ACTGTAATGG AAACGTACAT AAAGTCTGTA AAGATGCTGC CCCTCCGTGT
    ACCAAGAAAT TCCAAGACAA ATATAATAAG AACAAACCAC AGACCATCCT TGGAAATTCT
    TCGTTTCGAG ACGTCCCATT GCCTGGGCTC TCTCTGCACC CTTCCTCCTC CATGCCTATC
    GGGTTGCCAA CTGGGAGGAA GGAGATGGCA GGGCAAGTGC ATCCCTTGTC CAGAAGTGTC
    CCAGGCACCA CCTTGGAAAG CTTCAGGAGG CCGGGGGCAT CCTTGGAGTC CGAGAATGAT
    GGCAGCACCT GGAGAAGCAG GTCTCACTCT GACGAGCTGT TCCAGCCCAT GGGCTCGTCC
    CCCTCCACGG ACTCCTTCTT AACGGAAGAT GTCGTGGATT CTTCTCTGTG GAGTGACCTC
    AGCGGCGATG CCCAGGAGTT TGAAGCGGAA TCTTGGAGTC TGGTGGTGGA CCCTTCATTT
    TGTAGTCGGC AGGAGAAGGA TGTCATCCAA AGACAGGATG TCATTTTTGA GCTAATGCAG
    ACAGAACTGC ATCACATCCA GACCCTGTTC ATCATGTCTG AGATCTTCAG GAAAGGGATG
    AAAGAGGAGC TGCAGCTGGA CCACAGCACT GTGGACAAAA TCTTCCCCTG TCTGGACGAG
    TTACTTGAAA TCCACAAGCA TTTCTTCTGT AGTATGAAGG AGCGAAGGCA GGAATCCTGT
    GTTGGCAATG ACAGGAATTT CATCATCGGC CGAATTGGAG ATATTCTAGT ACAACAGTTT
    TCAGAAGAAA ATGCAAATAA AATGAAGAAA ATATATGGGG AATTCTGTAG CCATCACAAA
    GAGGCTGTTA GCCTCTTTAA AGAACTCCAG CAGAATAAAA AGTTTCAGAA TTTTATTAAG
    CTCCGAAATA GTAACCTTCT GGCTCGACGC CGAGGAATTC CAGAATGCAT TCTGCTGGTC
    ACTCAGCGCA TCACAAAATA CCCTGTCTTG GTGGAAAGGA TATTGCAGTA CACAAAGGAG
    AGCACTGAAG AACAGAGAGA CTTATGCAAA GCTCTTTGCT TAATTAAAGA CATGATTGCA
    GCTGTGGATT TAAAAGTCAG CGAATATGAG AAAAAACAAA AATGGCTTGA GATCCTAAAT
    AAGATCGAAA ACAAAACATA CACCAAGCTC AAAAATGGCC ATGTGTTTAG GAAACAGGCA
    CTGATGAGTA AAGAGAGGAC CCTGTTATAT GATGGCCTTG TCTATTGGAA AACTGCTACA
    GGTCGTTTCA AAGATATCCT AGCTCTACTT CTAACTGATG TGCTGCTCTT TTTACAAGAA
    AAAGACCAGA AATACATCTT TGCAGCTGTT GATCAGAAGC CATCAGTTAT TTCCCTTCAG
    AAGCTCATTG CTAGGGAAGT TGCGAATGAG GAGAGAGGAA TGTTTTTGAT CAGCGCTTCA
    TCTGCTGGTC CCGAGATGTA TGAAATCCAT ACCAACTCCA AGGAGGAAAG AAATACCTGG
    ATGAGACGGA TCCAACAGGC AGTAGAAAGT TGCCCAGAAG AAGAAGGGGG AAGGACAAGT
    GAGTCTGATG AAGAGAGGAG GAAAGCTGAA GCCAGAGTGG CGAAGATTCA GCAATGCCAA
    GAAATACTCA GTAACCAAGA TCAACAAATT TGCACATATT TGGAAGAGAA ACTGCATATC
    TACGCGAAAC TTGGAGAACT AAGTGGATTT CAGGATGTCC ATCTAGAGCC CCACCTCCTC
    ATTCAACCAG ACCCCGGAGA GCCTCCCCAG GCAGCCTCAT TACTGGCCGC AGCACTGAAA
    GAGGCTGAGA GTCTGCAGGT GGCAGTGAAG GCTTCCCAGA TGAGCGATGC ATGCCAGTCC
    CCCGAGGAAG GTTGTGGAGA ACCTGCTTTG CCGGACGTGC TCCATTCTCA GGACGCACCA
    GGATCGTCTA CTGCTTCACT AGTCACAGAA GGGACAGGAG GAAGAGGCTG TTGGGATGTG
    GATGCCGGGA CCCAGGGTGT GGGAACCGAC CTGGCGGTTT CTGATGCGGG GGAGAAGGTG
    GAATATAGAA GTTTCCCAGG TTCTTTGCAG TCAGAGATTA TACAAGCGAT ACAGAATTTA
    ACTCGTCTCT TATATAGCCT TCAGGCCGCC TTGACCATTC AGGATAGCCA CATTGAGATC
    CACAGGCTGG TTCTCCAACA GCGGGAGAGC CTGGCCCCGG GCCCCGCGTT CCGAGGCAGC
    CCCTTTCAGG ACCAGGAGAA GTCCCGCAGC CTGGAGAAGC AGCGGGAGGA GCTGGCCGAT
    ACCCACAAGC TGCAGCTCCA GTTCCAGCAA GACCAGCACC GCTGGCTCCG CAGGTGCGAC
    CAGCAGCAGC GCGAGCAGGA GGCCCGGGCC AGCCGGCTGC AGGAGCGCGA GAGGGAGTGC
    CGGGCGCAGG AGGAGCTGCT GCTCAGGAGC CGGGGCGAGC TGGACCAGCA GCTCCAAGAC
    TACCAGCAGA GCCTGGAGCG CCTGCGCGAG GGCCAGCGCC TGGTGGAGAG GGAGCGGGAG
    AGGATGCGGG CCCAGCAGAG CCTGCTGCGC CACTGGAAGC ACGGGCGGCA GAGCAGCCTT
    CCCGCCGCGT TTTCTCCAGG AGCCCTGGAG GTAATGGAAC TTAATCGATC TGAGACTTTA
    CGTCATGAAA ATTCATTCTT CATCAATGAA GCTTTAGTGC AAATGTCACT CAACCCTTTG
    AACAAACCGC CGCCACCAGG TATCCATCAG GATGCGGCTT ACCCCCCAGG CATACCTCAT
    TCTGATTTGG TAAGGACTAC TGAAAATCAA GTAGACCTCA AGACGGACGT TTCTCAACCC
    TCAGATATCA ACCATGAACT GTGGATAGCG GCTGGGGCCT GTCATCAGAT ACCCCCTCTC
    CACCAAAGCA GCAAGGATTC TTGTAAAAAT GATGTGAACA TCTCTCAGAC TGCATACCCA
    CCCCTCCATG ACTCAAATCC ACACAGCCCT CGGCTGCAGA CGTTTATAGC AGAAGAAAAG
    CTAAATCTAC AGAGAGTGAC CAGACAAGAC GGGGAAACTG GAAATGGAGC AGAAGAAAAT
    ATTGTTTACC TCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa228086
    Clone ID Related Accession (Same CDS sequence) XM_024981402.1
    Accession Version XM_024981402.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5121bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product rho guanine nucleotide exchange factor 28 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGCAGCCCA CTCTTCACTT TCTTCCCATC TTTAGGTGTC CGTTCAGCTG TGAGCTCTGT 
    GAGGAGTGGA TCGTTTCCTG GTCATCTTTG TGCAATGCTG AGCCCATTGA AGATGCGGAA
    ACCATGGAGC TGAACTGCAG CGAAGTACCT CTGTATGGGC AGATGACGGT CTGCGCGAAG
    TTTGGCAAAA ATGTTTATCT TCCTAAAGAT GCTGAGTTTT ACTTTATTTA TAATGGATCT
    CATCAGAGAC ACATCGTGAT TGCAGAGCGC ACAGAAGATA ATGTTCTCCA GTCCAGCATT
    CCAGGCCACG GGCTTCAGGA GACGGTCACC GTGTCTGTGT GCCTGTGCTC GGAAGGTTAC
    TCCCCGGTGA CCATGGGCTC TGACTCCGTG ACCTACGTGG ACAACATGGC CTGCAGGCTG
    GCCCGTCTGC TGGTCACCCA GGCCGATCGC CTCACCGCCA GCAGCCACCA GACCTTGCTG
    ACCCCGTTTG CCCTGACGGC GGGGGCATTG CCTGCTCTGG ACGAGGAGCT CGTGCTGGCC
    CTGACTCATC TGGAGTTGCC TCTGGGGTGG ACCGTCTTGG GGAATTCTTC ACTGGAAGTT
    TCTCTGCATA GGGAGTCTCT TCTGCACCTG GCTATGAGAT GGGGCCTTGC TAAGCTTTCC
    CAGCTCCTTC TGTGTCTCCC TGGGGGAGCC CAGGCCCTGA CTTTACCCAA CGAGGAGGGT
    GCCACGCCAT TAGACTTGGC TTTACATGGT GGACATTCCA AGCTGGTTGA AGACATCACA
    AACTTCCAGG GCAGGCGTTC CCCAGGTTTC TCCTGCGTGC AGCTCAGCGA AGATGCCTCT
    CTGCGGTATA TTCACTCATC AGAAACTCTG ACCCTGACGC TTAACCACAC AGCCGAGCAT
    TTGCTGGAGG CGGATATTAA ACTCTTCAGA AAATACTTTT GGGATAGAGC CTTTCTCATC
    AAGGCTCTTG AAGAGCAAGA AGGCACGTCA GAGGAGAAAC CAGATATGCC CTCCAGCGCT
    GCAGAAACTG AAGAAGATCG TTCCTTTGAT GCCCTCAAAA AATCCAAGCA CCCCTCAACA
    TTGCTTGCTG CTGGCCAGCT CTCTGACATG CTGAATGGTG GTGATGACGT CTACGCTAAC
    TGCATGGTGA TAGACCAGGT TGGTGATTTG GACATTAACT ATATTAATAT AGAGGAAATC
    CCTGCAAATA CCTGTCTTGA ATCCATGGAT TCTTCTCTGG GGCCTGAGAG CAGCAAACCC
    AGCCTTCCTA CTGAAGCCAG TGCTGGCACA TACCCACCAA ATAAGAGGCC AGAAGTGACG
    TCACACACGG AAGCCCAATC ATCTTTCATA TCATCTAGTT CGTATGCTTC CAATTTGAAT
    CTTTCTTTTG GTCTTCGTGG ATTTGAAAAG GAGCAAAGTC GTCTAAAGAA AAGAAGTTCC
    AGCCTTGATG CCTTGGATGC TGACAGTGAA GGAGAGGGCC ATTCGGAGCA GTCGCACAAT
    TGCTACACTC CCGTGTGTCA GAGTTCCTCG AGAACTGGCA TTGCTAGCGG GGATGAATTG
    GACTCTTTTG AGACCAACAC TGAACCAGAT TTTAATATCT CCAGGACGGA ATCATTTTCT
    TTATCAAGTA GCCTGCAGCT GAAGGAATCA TTGTTTTCTG GAATCCGTTC ACGTTCGTAT
    TCCTGCTCAT CACCCAAAAT TTCTTTAGGA AAAACTCGAT TGATGCGTGA TTTTACAGTG
    TGTAATTCAA TTGAAGAGCA AAGAGCTTAC AGCTTGCCGG AGCCACCGAG AGAACAAAGA
    ATTCAGGAAG AAGAATGGGA TAAATATATC ATACCTGCCA AATCAGAATC TGAAAAATAC
    AAAGTAAGCC GAACTTTTAG CTTCCTCATG AATAGGATGA CCAGCCCTCG GAATAAATCA
    AAGACAAAAG GCAAGGATGC CAAAGACAAA GAGAAACTGA CTCGACATCA GTTTGTCCCT
    GGAACTTTCT CTGGGGTTCT GCAGTGTTTG GTTTGCGATA AAACGCTCCT GGGGAAAGAG
    TCATTCCAGT GCTCCAACTG TAATGGAAAC GTACATAAAG TCTGTAAAGA TGCTGCCCCT
    CCGTGTACCA AGAAATTCCA AGACAAATAT AATAAGAACA AACCACAGAC CATCCTTGGA
    AATTCTTCGT TTCGAGACGT CCCATTGCCT GGGCTCTCTC TGCACCCTTC CTCCTCCATG
    CCTATCGGGT TGCCAACTGG GAGGAAGGAG ATGGCAGGGC AAGTGCATCC CTTGTCCAGA
    AGTGTCCCAG GCACCACCTT GGAAAGCTTC AGGAGGCCGG GGGCATCCTT GGAGTCCGAG
    AATGATGGCA GCACCTGGAG AAGCAGGTCT CACTCTGACG AGCTGTTCCA GCCCATGGGC
    TCGTCCCCCT CCACGGACTC CTTCTTAACG GAAGATGTCG TGGATTCTTC TCTGTGGAGT
    GACCTCAGCG GCGATGCCCA GGAGTTTGAA GCGGAATCTT GGAGTCTGGT GGTGGACCCT
    TCATTTTGTA GTCGGCAGGA GAAGGATGTC ATCCAAAGAC AGGATGTCAT TTTTGAGCTA
    ATGCAGACAG AACTGCATCA CATCCAGACC CTGTTCATCA TGTCTGAGAT CTTCAGGAAA
    GGGATGAAAG AGGAGCTGCA GCTGGACCAC AGCACTGTGG ACAAAATCTT CCCCTGTCTG
    GACGAGTTAC TTGAAATCCA CAAGCATTTC TTCTGTAGTA TGAAGGAGCG AAGGCAGGAA
    TCCTGTGTTG GCAATGACAG GAATTTCATC ATCGGCCGAA TTGGAGATAT TCTAGTACAA
    CAGTTTTCAG AAGAAAATGC AAATAAAATG AAGAAAATAT ATGGGGAATT CTGTAGCCAT
    CACAAAGAGG CTGTTAGCCT CTTTAAAGAA CTCCAGCAGA ATAAAAAGTT TCAGAATTTT
    ATTAAGCTCC GAAATAGTAA CCTTCTGGCT CGACGCCGAG GAATTCCAGA ATGCATTCTG
    CTGGTCACTC AGCGCATCAC AAAATACCCT GTCTTGGTGG AAAGGATATT GCAGTACACA
    AAGGAGAGCA CTGAAGAACA GAGAGACTTA TGCAAAGCTC TTTGCTTAAT TAAAGACATG
    ATTGCAGCTG TGGATTTAAA AGTCAGCGAA TATGAGAAAA AACAAAAATG GCTTGAGATC
    CTAAATAAGA TCGAAAACAA AACATACACC AAGCTCAAAA ATGGCCATGT GTTTAGGAAA
    CAGGCACTGA TGAGTAAAGA GAGGACCCTG TTATATGATG GCCTTGTCTA TTGGAAAACT
    GCTACAGGTC GTTTCAAAGA TATCCTAGCT CTACTTCTAA CTGATGTGCT GCTCTTTTTA
    CAAGAAAAAG ACCAGAAATA CATCTTTGCA GCTGTTGATC AGAAGCCATC AGTTATTTCC
    CTTCAGAAGC TCATTGCTAG GGAAGTTGCG AATGAGGAGA GAGGAATGTT TTTGATCAGC
    GCTTCATCTG CTGGTCCCGA GATGTATGAA ATCCATACCA ACTCCAAGGA GGAAAGAAAT
    ACCTGGATGA GACGGATCCA ACAGGCAGTA GAAAGTTGCC CAGAAGAAGA AGGGGGAAGG
    ACAAGTGAGT CTGATGAAGA GAGGAGGAAA GCTGAAGCCA GAGTGGCGAA GATTCAGCAA
    TGCCAAGAAA TACTCAGTAA CCAAGATCAA CAAATTTGCA CATATTTGGA AGAGAAACTG
    CATATCTACG CGAAACTTGG AGAACTAAGT GGATTTCAGG ATGTCCATCT AGAGCCCCAC
    CTCCTCATTC AACCAGACCC CGGAGAGCCT CCCCAGGCAG CCTCATTACT GGCCGCAGCA
    CTGAAAGAGG CTGAGAGTCT GCAGGTGGCA GTGAAGGCTT CCCAGATGAG CGATGCATGC
    CAGTCCCCCG AGGAAGGTTG TGGAGAACCT GCTTTGCCGG ACGTGCTCCA TTCTCAGGAC
    GCACCAGGAT CGTCTACTGC TTCACTAGTC ACAGAAGGGA CAGGAGGAAG AGGCTGTTGG
    GATGTGGATG CCGGGACCCA GGGTGTGGGA ACCGACCTGG CGGTTTCTGA TGCGGGGGAG
    AAGGTGGAAT ATAGAAGTTT CCCAGGTTCT TTGCAGTCAG AGATTATACA AGCGATACAG
    AATTTAACTC GTCTCTTATA TAGCCTTCAG GCCGCCTTGA CCATTCAGGA TAGCCACATT
    GAGATCCACA GGCTGGTTCT CCAACAGCGG GAGAGCCTGG CCCCGGGCCC CGCGTTCCGA
    GGCAGCCCCT TTCAGGACCA GGAGAAGTCC CGCAGCCTGG AGAAGCAGCG GGAGGAGCTG
    GCCGATACCC ACAAGCTGCA GCTCCAGTTC CAGCAAGACC AGCACCGCTG GCTCCGCAGG
    TGCGACCAGC AGCAGCGCGA GCAGGAGGCC CGGGCCAGCC GGCTGCAGGA GCGCGAGAGG
    GAGTGCCGGG CGCAGGAGGA GCTGCTGCTC AGGAGCCGGG GCGAGCTGGA CCAGCAGCTC
    CAAGACTACC AGCAGAGCCT GGAGCGCCTG CGCGAGGGCC AGCGCCTGGT GGAGAGGGAG
    CGGGAGAGGA TGCGGGCCCA GCAGAGCCTG CTGCGCCACT GGAAGCACGG GCGGCAGAGC
    AGCCTTCCCG CCGCGTTTTC TCCAGGAGCC CTGGAGGTAA TGGAACTTAA TCGATCTGAG
    ACTTTACGTC ATGAAAATTC ATTCTTCATC AATGAAGCTT TAGTGCAAAT GTCACTCAAC
    CCTTTGAACA AACCGCCGCC ACCAGGTATC CATCAGGATG CGGCTTACCC CCCAGGCATA
    CCTCATTCTG ATTTGGTAAG GACTACTGAA AATCAAGTAG ACCTCAAGAC GGACGTTTCT
    CAACCCTCAG ATATCAACCA TGAACTGTGG ATAGCGGCTG GGGCCTGTCA TCAGATACCC
    CCTCTCCACC AAAGCAGCAA GGATTCTTGT AAAAATGATG TGAACATCTC TCAGACTGCA
    TACCCACCCC TCCATGACTC AAATCCACAC AGCCCTCGGC TGCAGACGTT TATAGCAGAA
    GAAAAGCTAA ATCTACAGAG AGTGACCAGA CAAGACGGGG AAACTGGAAA TGGAGCAGAA
    GAAAATATTG TTTACCTCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024837170.1
    CDS16..5136
    Translation

    Target ORF information:

    RefSeq Version XM_024981402.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981402.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGCAGCCCA CTCTTCACTT TCTTCCCATC TTTAGGTGTC CGTTCAGCTG TGAGCTCTGT 
    GAGGAGTGGA TCGTTTCCTG GTCATCTTTG TGCAATGCTG AGCCCATTGA AGATGCGGAA
    ACCATGGAGC TGAACTGCAG CGAAGTACCT CTGTATGGGC AGATGACGGT CTGCGCGAAG
    TTTGGCAAAA ATGTTTATCT TCCTAAAGAT GCTGAGTTTT ACTTTATTTA TAATGGATCT
    CATCAGAGAC ACATCGTGAT TGCAGAGCGC ACAGAAGATA ATGTTCTCCA GTCCAGCATT
    CCAGGCCACG GGCTTCAGGA GACGGTCACC GTGTCTGTGT GCCTGTGCTC GGAAGGTTAC
    TCCCCGGTGA CCATGGGCTC TGACTCCGTG ACCTACGTGG ACAACATGGC CTGCAGGCTG
    GCCCGTCTGC TGGTCACCCA GGCCGATCGC CTCACCGCCA GCAGCCACCA GACCTTGCTG
    ACCCCGTTTG CCCTGACGGC GGGGGCATTG CCTGCTCTGG ACGAGGAGCT CGTGCTGGCC
    CTGACTCATC TGGAGTTGCC TCTGGGGTGG ACCGTCTTGG GGAATTCTTC ACTGGAAGTT
    TCTCTGCATA GGGAGTCTCT TCTGCACCTG GCTATGAGAT GGGGCCTTGC TAAGCTTTCC
    CAGCTCCTTC TGTGTCTCCC TGGGGGAGCC CAGGCCCTGA CTTTACCCAA CGAGGAGGGT
    GCCACGCCAT TAGACTTGGC TTTACATGGT GGACATTCCA AGCTGGTTGA AGACATCACA
    AACTTCCAGG GCAGGCGTTC CCCAGGTTTC TCCTGCGTGC AGCTCAGCGA AGATGCCTCT
    CTGCGGTATA TTCACTCATC AGAAACTCTG ACCCTGACGC TTAACCACAC AGCCGAGCAT
    TTGCTGGAGG CGGATATTAA ACTCTTCAGA AAATACTTTT GGGATAGAGC CTTTCTCATC
    AAGGCTCTTG AAGAGCAAGA AGGCACGTCA GAGGAGAAAC CAGATATGCC CTCCAGCGCT
    GCAGAAACTG AAGAAGATCG TTCCTTTGAT GCCCTCAAAA AATCCAAGCA CCCCTCAACA
    TTGCTTGCTG CTGGCCAGCT CTCTGACATG CTGAATGGTG GTGATGACGT CTACGCTAAC
    TGCATGGTGA TAGACCAGGT TGGTGATTTG GACATTAACT ATATTAATAT AGAGGAAATC
    CCTGCAAATA CCTGTCTTGA ATCCATGGAT TCTTCTCTGG GGCCTGAGAG CAGCAAACCC
    AGCCTTCCTA CTGAAGCCAG TGCTGGCACA TACCCACCAA ATAAGAGGCC AGAAGTGACG
    TCACACACGG AAGCCCAATC ATCTTTCATA TCATCTAGTT CGTATGCTTC CAATTTGAAT
    CTTTCTTTTG GTCTTCGTGG ATTTGAAAAG GAGCAAAGTC GTCTAAAGAA AAGAAGTTCC
    AGCCTTGATG CCTTGGATGC TGACAGTGAA GGAGAGGGCC ATTCGGAGCA GTCGCACAAT
    TGCTACACTC CCGTGTGTCA GAGTTCCTCG AGAACTGGCA TTGCTAGCGG GGATGAATTG
    GACTCTTTTG AGACCAACAC TGAACCAGAT TTTAATATCT CCAGGACGGA ATCATTTTCT
    TTATCAAGTA GCCTGCAGCT GAAGGAATCA TTGTTTTCTG GAATCCGTTC ACGTTCGTAT
    TCCTGCTCAT CACCCAAAAT TTCTTTAGGA AAAACTCGAT TGATGCGTGA TTTTACAGTG
    TGTAATTCAA TTGAAGAGCA AAGAGCTTAC AGCTTGCCGG AGCCACCGAG AGAACAAAGA
    ATTCAGGAAG AAGAATGGGA TAAATATATC ATACCTGCCA AATCAGAATC TGAAAAATAC
    AAAGTAAGCC GAACTTTTAG CTTCCTCATG AATAGGATGA CCAGCCCTCG GAATAAATCA
    AAGACAAAAG GCAAGGATGC CAAAGACAAA GAGAAACTGA CTCGACATCA GTTTGTCCCT
    GGAACTTTCT CTGGGGTTCT GCAGTGTTTG GTTTGCGATA AAACGCTCCT GGGGAAAGAG
    TCATTCCAGT GCTCCAACTG TAATGGAAAC GTACATAAAG TCTGTAAAGA TGCTGCCCCT
    CCGTGTACCA AGAAATTCCA AGACAAATAT AATAAGAACA AACCACAGAC CATCCTTGGA
    AATTCTTCGT TTCGAGACGT CCCATTGCCT GGGCTCTCTC TGCACCCTTC CTCCTCCATG
    CCTATCGGGT TGCCAACTGG GAGGAAGGAG ATGGCAGGGC AAGTGCATCC CTTGTCCAGA
    AGTGTCCCAG GCACCACCTT GGAAAGCTTC AGGAGGCCGG GGGCATCCTT GGAGTCCGAG
    AATGATGGCA GCACCTGGAG AAGCAGGTCT CACTCTGACG AGCTGTTCCA GCCCATGGGC
    TCGTCCCCCT CCACGGACTC CTTCTTAACG GAAGATGTCG TGGATTCTTC TCTGTGGAGT
    GACCTCAGCG GCGATGCCCA GGAGTTTGAA GCGGAATCTT GGAGTCTGGT GGTGGACCCT
    TCATTTTGTA GTCGGCAGGA GAAGGATGTC ATCCAAAGAC AGGATGTCAT TTTTGAGCTA
    ATGCAGACAG AACTGCATCA CATCCAGACC CTGTTCATCA TGTCTGAGAT CTTCAGGAAA
    GGGATGAAAG AGGAGCTGCA GCTGGACCAC AGCACTGTGG ACAAAATCTT CCCCTGTCTG
    GACGAGTTAC TTGAAATCCA CAAGCATTTC TTCTGTAGTA TGAAGGAGCG AAGGCAGGAA
    TCCTGTGTTG GCAATGACAG GAATTTCATC ATCGGCCGAA TTGGAGATAT TCTAGTACAA
    CAGTTTTCAG AAGAAAATGC AAATAAAATG AAGAAAATAT ATGGGGAATT CTGTAGCCAT
    CACAAAGAGG CTGTTAGCCT CTTTAAAGAA CTCCAGCAGA ATAAAAAGTT TCAGAATTTT
    ATTAAGCTCC GAAATAGTAA CCTTCTGGCT CGACGCCGAG GAATTCCAGA ATGCATTCTG
    CTGGTCACTC AGCGCATCAC AAAATACCCT GTCTTGGTGG AAAGGATATT GCAGTACACA
    AAGGAGAGCA CTGAAGAACA GAGAGACTTA TGCAAAGCTC TTTGCTTAAT TAAAGACATG
    ATTGCAGCTG TGGATTTAAA AGTCAGCGAA TATGAGAAAA AACAAAAATG GCTTGAGATC
    CTAAATAAGA TCGAAAACAA AACATACACC AAGCTCAAAA ATGGCCATGT GTTTAGGAAA
    CAGGCACTGA TGAGTAAAGA GAGGACCCTG TTATATGATG GCCTTGTCTA TTGGAAAACT
    GCTACAGGTC GTTTCAAAGA TATCCTAGCT CTACTTCTAA CTGATGTGCT GCTCTTTTTA
    CAAGAAAAAG ACCAGAAATA CATCTTTGCA GCTGTTGATC AGAAGCCATC AGTTATTTCC
    CTTCAGAAGC TCATTGCTAG GGAAGTTGCG AATGAGGAGA GAGGAATGTT TTTGATCAGC
    GCTTCATCTG CTGGTCCCGA GATGTATGAA ATCCATACCA ACTCCAAGGA GGAAAGAAAT
    ACCTGGATGA GACGGATCCA ACAGGCAGTA GAAAGTTGCC CAGAAGAAGA AGGGGGAAGG
    ACAAGTGAGT CTGATGAAGA GAGGAGGAAA GCTGAAGCCA GAGTGGCGAA GATTCAGCAA
    TGCCAAGAAA TACTCAGTAA CCAAGATCAA CAAATTTGCA CATATTTGGA AGAGAAACTG
    CATATCTACG CGAAACTTGG AGAACTAAGT GGATTTCAGG ATGTCCATCT AGAGCCCCAC
    CTCCTCATTC AACCAGACCC CGGAGAGCCT CCCCAGGCAG CCTCATTACT GGCCGCAGCA
    CTGAAAGAGG CTGAGAGTCT GCAGGTGGCA GTGAAGGCTT CCCAGATGAG CGATGCATGC
    CAGTCCCCCG AGGAAGGTTG TGGAGAACCT GCTTTGCCGG ACGTGCTCCA TTCTCAGGAC
    GCACCAGGAT CGTCTACTGC TTCACTAGTC ACAGAAGGGA CAGGAGGAAG AGGCTGTTGG
    GATGTGGATG CCGGGACCCA GGGTGTGGGA ACCGACCTGG CGGTTTCTGA TGCGGGGGAG
    AAGGTGGAAT ATAGAAGTTT CCCAGGTTCT TTGCAGTCAG AGATTATACA AGCGATACAG
    AATTTAACTC GTCTCTTATA TAGCCTTCAG GCCGCCTTGA CCATTCAGGA TAGCCACATT
    GAGATCCACA GGCTGGTTCT CCAACAGCGG GAGAGCCTGG CCCCGGGCCC CGCGTTCCGA
    GGCAGCCCCT TTCAGGACCA GGAGAAGTCC CGCAGCCTGG AGAAGCAGCG GGAGGAGCTG
    GCCGATACCC ACAAGCTGCA GCTCCAGTTC CAGCAAGACC AGCACCGCTG GCTCCGCAGG
    TGCGACCAGC AGCAGCGCGA GCAGGAGGCC CGGGCCAGCC GGCTGCAGGA GCGCGAGAGG
    GAGTGCCGGG CGCAGGAGGA GCTGCTGCTC AGGAGCCGGG GCGAGCTGGA CCAGCAGCTC
    CAAGACTACC AGCAGAGCCT GGAGCGCCTG CGCGAGGGCC AGCGCCTGGT GGAGAGGGAG
    CGGGAGAGGA TGCGGGCCCA GCAGAGCCTG CTGCGCCACT GGAAGCACGG GCGGCAGAGC
    AGCCTTCCCG CCGCGTTTTC TCCAGGAGCC CTGGAGGTAA TGGAACTTAA TCGATCTGAG
    ACTTTACGTC ATGAAAATTC ATTCTTCATC AATGAAGCTT TAGTGCAAAT GTCACTCAAC
    CCTTTGAACA AACCGCCGCC ACCAGGTATC CATCAGGATG CGGCTTACCC CCCAGGCATA
    CCTCATTCTG ATTTGGTAAG GACTACTGAA AATCAAGTAG ACCTCAAGAC GGACGTTTCT
    CAACCCTCAG ATATCAACCA TGAACTGTGG ATAGCGGCTG GGGCCTGTCA TCAGATACCC
    CCTCTCCACC AAAGCAGCAA GGATTCTTGT AAAAATGATG TGAACATCTC TCAGACTGCA
    TACCCACCCC TCCATGACTC AAATCCACAC AGCCCTCGGC TGCAGACGTT TATAGCAGAA
    GAAAAGCTAA ATCTACAGAG AGTGACCAGA CAAGACGGGG AAACTGGAAA TGGAGCAGAA
    GAAAATATTG TTTACCTCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa228087
    Clone ID Related Accession (Same CDS sequence) XM_024981403.1
    Accession Version XM_024981403.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4977bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product rho guanine nucleotide exchange factor 28 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCAGCCCA CTCTTCACTT TCTTCCCATC TTTAGGTGTC CGTTCAGCTG TGAGCTCTGT 
    GAGGAGTGGA TCGTTTCCTG GTCATCTTTG TGCAATGCTG AGCCCATTGA AGATGCGGAA
    ACCATGGAGC TGAACTGCAG CGAAGTACCT CTGTATGGGC AGATGACGGT CTGCGCGAAG
    TTTGGCAAAA ATGTTTATCT TCCTAAAGAT GCTGAGTTTT ACTTTATTTA TAATGGATCT
    CATCAGAGAC ACATCGTGAT TGCAGAGCGC ACAGAAGATA ATGTTCTCCA GTCCAGCATT
    CCAGGCCACG GGCTTCAGGA GACGGTCACC GTGTCTGTGT GCCTGTGCTC GGAAGGTTAC
    TCCCCGGTGA CCATGGGCTC TGACTCCGTG ACCTACGTGG ACAACATGGC CTGCAGGCTG
    GCCCGTCTGC TGGTCACCCA GGCCGATCGC CTCACCGCCA GCAGCCACCA GACCTTGCTG
    ACCCCGTTTG CCCTGACGGC GGGGGCATTG CCTGCTCTGG ACGAGGAGCT CGTGCTGGCC
    CTGACTCATC TGGAGTTGCC TCTGGGGTGG ACCGTCTTGG GGAATTCTTC ACTGGAAGTT
    TCTCTGCATA GGGAGTCTCT TCTGCACCTG GCTATGAGAT GGGGCCTTGC TAAGCTTTCC
    CAGCTCCTTC TGTGTCTCCC TGGGGGAGCC CAGGCCCTGA CTTTACCCAA CGAGGAGGGT
    GCCACGCCAT TAGACTTGGC TTTACATGGT GGACATTCCA AGCTGGTTGA AGACATCACA
    AACTTCCAGG GCAGGCGTTC CCCAGGTTTC TCCTGCGTGC AGCTCAGCGA AGATGCCTCT
    CTGCGGTATA TTCACTCATC AGAAACTCTG ACCCTGACGC TTAACCACAC AGCCGAGCAT
    TTGCTGGAGG CGGATATTAA ACTCTTCAGA AAATACTTTT GGGATAGAGC CTTTCTCATC
    AAGGCTCTTG AAGAGCAAGA AGGCACGTCA GAGGAGAAAC CAGATATGCC CTCCAGCGCT
    GCAGAAACTG AAGAAGGTAT GCATGCTCCT TCCCCACCTG GGGAACTCAG AAAGTGCTCA
    GCATTCTCTA GTCAATTGCG AGTTGAAGTG TGTGGAGAAG GAGAGCAGGA GCCGATGGAC
    TCGGAGGCGA GAGTAGGTTG CTCTGGGGCC GCAGGGCCCA GAGTTCCTTC AGCAGCACTG
    ACCAAAGAGA TTAAGAATTC GGGGTGCAGC AGATCACCAC CCGGGAAGGA GGGTGTAAAG
    CGTGTGAAAA GCCTGGTGGT TCCACTGAAT GAACATGAGG ACCAGGAGAG GCTAGATTTG
    GATCGTTCCT TTGATGCCCT CAAAAAATCC AAGCACCCCT CAACATTGCT TGCTGCTGGC
    CAGCTCTCTG ACATGCTGAA TGGTGGTGAT GACGTCTACG CTAACTGCAT GGTGATAGAC
    CAGGTTGGTG ATTTGGACAT TAACTATATT AATATAGAGG AAATCCCTGC AAATACCTGT
    CTTGAATCCA TGGATTCTTC TCTGGGGCCT GAGAGCAGCA AACCCAGCCT TCCTACTGAA
    GCCAGTGCTG GCACATACCC ACCAAATAAG AGGCCAGAAG TGACGTCACA CACGGAAGCC
    CAATCATCTT TCATATCATC TAGTTCGTAT GCTTCCAATT TGAATCTTTC TTTTGGTCTT
    CGTGGATTTG AAAAGGAGCA AAGTCGTCTA AAGAAAAGAA GTTCCAGCCT TGATGCCTTG
    GATGCTGACA GTGAAGGAGA GGGCCATTCG GAGCAGTCGC ACAATTGCTA CACTCCCGTG
    TGTCAGAGTT CCTCGAGAAC TGGCATTGCT AGCGGGGATG AATTGGACTC TTTTGAGACC
    AACACTGAAC CAGATTTTAA TATCTCCAGG ACGGAATCAT TTTCTTTATC AAGTAGCCTG
    CAGCTGAAGG AATCATTGTT TTCTGGAATC CGTTCACGTT CGTATTCCTG CTCATCACCC
    AAAATTTCTT TAGGAAAAAC TCGATTGATG CGTGATTTTA CAGTGTGTAA TTCAATTGAA
    GAGCAAAGAG CTTACAGCTT GCCGGAGCCA CCGAGAGAAC AAAGAATTCA GGAAGAAGAA
    TGGGATAAAT ATATCATACC TGCCAAATCA GAATCTGAAA AATACAAAGT AAGCCGAACT
    TTTAGCTTCC TCATGAATAG GATGACCAGC CCTCGGAATA AATCAAAGAC AAAAGGCAAG
    GATGCCAAAG ACAAAGAGAA ACTGACTCGA CATCAGTTTG TCCCTGGAAC TTTCTCTGGG
    GTTCTGCAGT GTTTGGTTTG CGATAAAACG CTCCTGGGGA AAGAGTCATT CCAGTGCTCC
    AACTGTAATG GAAACGTACA TAAAGTCTGT AAAGATGCTG CCCCTCCGTG TACCAAGAAA
    TTCCAAGACA AATATAATAA GAACAAACCA CAGACCATCC TTGGAAATTC TTCGTTTCGA
    GACGTCCCAT TGCCTGGGCT CTCTCTGCAC CCTTCCTCCT CCATGCCTAT CGGGTTGCCA
    ACTGGGAGGA AGGAGATGGC AGGGCAAGTG CATCCCTTGT CCAGAAGTGT CCCAGGCACC
    ACCTTGGAAA GCTTCAGGAG GCCGGGGGCA TCCTTGGAGT CCGAGAATGA TGGCAGCACC
    TGGAGAAGCA GGTCTCACTC TGACGAGCTG TTCCAGCCCA TGGGCTCGTC CCCCTCCACG
    GACTCCTTCT TAACGGAAGA TGTCGTGGAT TCTTCTCTGT GGAGTGACCT CAGCGGCGAT
    GCCCAGGAGT TTGAAGCGGA ATCTTGGAGT CTGGTGGTGG ACCCTTCATT TTGTAGTCGG
    CAGGAGAAGG ATGTCATCCA AAGACAGGAT GTCATTTTTG AGCTAATGCA GACAGAACTG
    CATCACATCC AGACCCTGTT CATCATGTCT GAGATCTTCA GGAAAGGGAT GAAAGAGGAG
    CTGCAGCTGG ACCACAGCAC TGTGGACAAA ATCTTCCCCT GTCTGGACGA GTTACTTGAA
    ATCCACAAGC ATTTCTTCTG TAGTATGAAG GAGCGAAGGC AGGAATCCTG TGTTGGCAAT
    GACAGGAATT TCATCATCGG CCGAATTGGA GATATTCTAG TACAACAGTT TTCAGAAGAA
    AATGCAAATA AAATGAAGAA AATATATGGG GAATTCTGTA GCCATCACAA AGAGGCTGTT
    AGCCTCTTTA AAGAACTCCA GCAGAATAAA AAGTTTCAGA ATTTTATTAA GCTCCGAAAT
    AGTAACCTTC TGGCTCGACG CCGAGGAATT CCAGAATGCA TTCTGCTGGT CACTCAGCGC
    ATCACAAAAT ACCCTGTCTT GGTGGAAAGG ATATTGCAGT ACACAAAGGA GAGCACTGAA
    GAACAGAGAG ACTTATGCAA AGCTCTTTGC TTAATTAAAG ACATGATTGC AGCTGTGGAT
    TTAAAAGTCA GCGAATATGA GAAAAAACAA AAATGGCTTG AGATCCTAAA TAAGATCGAA
    AACAAAACAT ACACCAAGCT CAAAAATGGC CATGTGTTTA GGAAACAGGC ACTGATGAGT
    AAAGAGAGGA CCCTGTTATA TGATGGCCTT GTCTATTGGA AAACTGCTAC AGGTCGTTTC
    AAAGATATCC TAGCTCTACT TCTAACTGAT GTGCTGCTCT TTTTACAAGA AAAAGACCAG
    AAATACATCT TTGCAGCTGT TGATCAGAAG CCATCAGTTA TTTCCCTTCA GAAGCTCATT
    GCTAGGGAAG TTGCGAATGA GGAGAGAGGA ATGTTTTTGA TCAGCGCTTC ATCTGCTGGT
    CCCGAGATGT ATGAAATCCA TACCAACTCC AAGGAGGAAA GAAATACCTG GATGAGACGG
    ATCCAACAGG CAGTAGAAAG TTGCCCAGAA GAAGAAGGGG GAAGGACAAG TGAGTCTGAT
    GAAGAGAGGA GGAAAGCTGA AGCCAGAGTG GCGAAGATTC AGCAATGCCA AGAAATACTC
    AGTAACCAAG ATCAACAAAT TTGCACATAT TTGGAAGAGA AACTGCATAT CTACGCGAAA
    CTTGGAGAAC TAAGTGGATT TCAGGATGTC CATCTAGAGC CCCACCTCCT CATTCAACCA
    GACCCCGGAG AGCCTCCCCA GGCAGCCTCA TTACTGGCCG CAGCACTGAA AGAGGCTGAG
    AGTCTGCAGG TGGCAGTGAA GGCTTCCCAG ATGAGCGATG CATGCCAGTC CCCCGAGGAA
    GGTTGTGGAG AACCTGCTTT GCCGGACGTG CTCCATTCTC AGGACGCACC AGGATCGTCT
    ACTGCTTCAC TAGTCACAGA AGGGACAGGA GGAAGAGGCT GTTGGGATGT GGATGCCGGG
    ACCCAGGGTG TGGGAACCGA CCTGGCGGTT TCTGATGCGG GGGAGAAGGT GGAATATAGA
    AGTTTCCCAG GTTCTTTGCA GTCAGAGATT ATACAAGCGA TACAGAATTT AACTCGTCTC
    TTATATAGCC TTCAGGCCGC CTTGACCATT CAGGATAGCC ACATTGAGAT CCACAGGCTG
    GTTCTCCAAC AGCGGGAGAG CCTGGCCCCG GGCCCCGCGT TCCGAGGCAG CCCCTTTCAG
    GACCAGGAGA AGTCCCGCAG CCTGGAGAAG CAGCGGGAGG AGCTGGCCGA TACCCACAAG
    CTGCAGCTCC AGTTCCAGCA AGACCAGCAC CGCTGGCTCC GCAGGTGCGA CCAGCAGCAG
    CGCGAGCAGG AGGCCCGGGC CAGCCGGCTG CAGGAGCGCG AGAGGGAGTG CCGGGCGCAG
    GAGGAGCTGC TGCTCAGGAG CCGGGGCGAG CTGGACCAGC AGCTCCAAGA CTACCAGCAG
    AGCCTGGAGC GCCTGCGCGA GGGCCAGCGC CTGGTGGAGA GGGAGCGGGA GAGGATGCGG
    GCCCAGCAGA GCCTGCTGCG CCACTGGAAG CACGGGCGGC AGAGCAGCCT TCCCGCCGCG
    TTTTCTCCAG GAGCCCTGGA GGTACGGGAC TCCTGTGCTC GCAGAAGCAG AGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024837171.1
    CDS121..5097
    Translation

    Target ORF information:

    RefSeq Version XM_024981403.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981403.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCAGCCCA CTCTTCACTT TCTTCCCATC TTTAGGTGTC CGTTCAGCTG TGAGCTCTGT 
    GAGGAGTGGA TCGTTTCCTG GTCATCTTTG TGCAATGCTG AGCCCATTGA AGATGCGGAA
    ACCATGGAGC TGAACTGCAG CGAAGTACCT CTGTATGGGC AGATGACGGT CTGCGCGAAG
    TTTGGCAAAA ATGTTTATCT TCCTAAAGAT GCTGAGTTTT ACTTTATTTA TAATGGATCT
    CATCAGAGAC ACATCGTGAT TGCAGAGCGC ACAGAAGATA ATGTTCTCCA GTCCAGCATT
    CCAGGCCACG GGCTTCAGGA GACGGTCACC GTGTCTGTGT GCCTGTGCTC GGAAGGTTAC
    TCCCCGGTGA CCATGGGCTC TGACTCCGTG ACCTACGTGG ACAACATGGC CTGCAGGCTG
    GCCCGTCTGC TGGTCACCCA GGCCGATCGC CTCACCGCCA GCAGCCACCA GACCTTGCTG
    ACCCCGTTTG CCCTGACGGC GGGGGCATTG CCTGCTCTGG ACGAGGAGCT CGTGCTGGCC
    CTGACTCATC TGGAGTTGCC TCTGGGGTGG ACCGTCTTGG GGAATTCTTC ACTGGAAGTT
    TCTCTGCATA GGGAGTCTCT TCTGCACCTG GCTATGAGAT GGGGCCTTGC TAAGCTTTCC
    CAGCTCCTTC TGTGTCTCCC TGGGGGAGCC CAGGCCCTGA CTTTACCCAA CGAGGAGGGT
    GCCACGCCAT TAGACTTGGC TTTACATGGT GGACATTCCA AGCTGGTTGA AGACATCACA
    AACTTCCAGG GCAGGCGTTC CCCAGGTTTC TCCTGCGTGC AGCTCAGCGA AGATGCCTCT
    CTGCGGTATA TTCACTCATC AGAAACTCTG ACCCTGACGC TTAACCACAC AGCCGAGCAT
    TTGCTGGAGG CGGATATTAA ACTCTTCAGA AAATACTTTT GGGATAGAGC CTTTCTCATC
    AAGGCTCTTG AAGAGCAAGA AGGCACGTCA GAGGAGAAAC CAGATATGCC CTCCAGCGCT
    GCAGAAACTG AAGAAGGTAT GCATGCTCCT TCCCCACCTG GGGAACTCAG AAAGTGCTCA
    GCATTCTCTA GTCAATTGCG AGTTGAAGTG TGTGGAGAAG GAGAGCAGGA GCCGATGGAC
    TCGGAGGCGA GAGTAGGTTG CTCTGGGGCC GCAGGGCCCA GAGTTCCTTC AGCAGCACTG
    ACCAAAGAGA TTAAGAATTC GGGGTGCAGC AGATCACCAC CCGGGAAGGA GGGTGTAAAG
    CGTGTGAAAA GCCTGGTGGT TCCACTGAAT GAACATGAGG ACCAGGAGAG GCTAGATTTG
    GATCGTTCCT TTGATGCCCT CAAAAAATCC AAGCACCCCT CAACATTGCT TGCTGCTGGC
    CAGCTCTCTG ACATGCTGAA TGGTGGTGAT GACGTCTACG CTAACTGCAT GGTGATAGAC
    CAGGTTGGTG ATTTGGACAT TAACTATATT AATATAGAGG AAATCCCTGC AAATACCTGT
    CTTGAATCCA TGGATTCTTC TCTGGGGCCT GAGAGCAGCA AACCCAGCCT TCCTACTGAA
    GCCAGTGCTG GCACATACCC ACCAAATAAG AGGCCAGAAG TGACGTCACA CACGGAAGCC
    CAATCATCTT TCATATCATC TAGTTCGTAT GCTTCCAATT TGAATCTTTC TTTTGGTCTT
    CGTGGATTTG AAAAGGAGCA AAGTCGTCTA AAGAAAAGAA GTTCCAGCCT TGATGCCTTG
    GATGCTGACA GTGAAGGAGA GGGCCATTCG GAGCAGTCGC ACAATTGCTA CACTCCCGTG
    TGTCAGAGTT CCTCGAGAAC TGGCATTGCT AGCGGGGATG AATTGGACTC TTTTGAGACC
    AACACTGAAC CAGATTTTAA TATCTCCAGG ACGGAATCAT TTTCTTTATC AAGTAGCCTG
    CAGCTGAAGG AATCATTGTT TTCTGGAATC CGTTCACGTT CGTATTCCTG CTCATCACCC
    AAAATTTCTT TAGGAAAAAC TCGATTGATG CGTGATTTTA CAGTGTGTAA TTCAATTGAA
    GAGCAAAGAG CTTACAGCTT GCCGGAGCCA CCGAGAGAAC AAAGAATTCA GGAAGAAGAA
    TGGGATAAAT ATATCATACC TGCCAAATCA GAATCTGAAA AATACAAAGT AAGCCGAACT
    TTTAGCTTCC TCATGAATAG GATGACCAGC CCTCGGAATA AATCAAAGAC AAAAGGCAAG
    GATGCCAAAG ACAAAGAGAA ACTGACTCGA CATCAGTTTG TCCCTGGAAC TTTCTCTGGG
    GTTCTGCAGT GTTTGGTTTG CGATAAAACG CTCCTGGGGA AAGAGTCATT CCAGTGCTCC
    AACTGTAATG GAAACGTACA TAAAGTCTGT AAAGATGCTG CCCCTCCGTG TACCAAGAAA
    TTCCAAGACA AATATAATAA GAACAAACCA CAGACCATCC TTGGAAATTC TTCGTTTCGA
    GACGTCCCAT TGCCTGGGCT CTCTCTGCAC CCTTCCTCCT CCATGCCTAT CGGGTTGCCA
    ACTGGGAGGA AGGAGATGGC AGGGCAAGTG CATCCCTTGT CCAGAAGTGT CCCAGGCACC
    ACCTTGGAAA GCTTCAGGAG GCCGGGGGCA TCCTTGGAGT CCGAGAATGA TGGCAGCACC
    TGGAGAAGCA GGTCTCACTC TGACGAGCTG TTCCAGCCCA TGGGCTCGTC CCCCTCCACG
    GACTCCTTCT TAACGGAAGA TGTCGTGGAT TCTTCTCTGT GGAGTGACCT CAGCGGCGAT
    GCCCAGGAGT TTGAAGCGGA ATCTTGGAGT CTGGTGGTGG ACCCTTCATT TTGTAGTCGG
    CAGGAGAAGG ATGTCATCCA AAGACAGGAT GTCATTTTTG AGCTAATGCA GACAGAACTG
    CATCACATCC AGACCCTGTT CATCATGTCT GAGATCTTCA GGAAAGGGAT GAAAGAGGAG
    CTGCAGCTGG ACCACAGCAC TGTGGACAAA ATCTTCCCCT GTCTGGACGA GTTACTTGAA
    ATCCACAAGC ATTTCTTCTG TAGTATGAAG GAGCGAAGGC AGGAATCCTG TGTTGGCAAT
    GACAGGAATT TCATCATCGG CCGAATTGGA GATATTCTAG TACAACAGTT TTCAGAAGAA
    AATGCAAATA AAATGAAGAA AATATATGGG GAATTCTGTA GCCATCACAA AGAGGCTGTT
    AGCCTCTTTA AAGAACTCCA GCAGAATAAA AAGTTTCAGA ATTTTATTAA GCTCCGAAAT
    AGTAACCTTC TGGCTCGACG CCGAGGAATT CCAGAATGCA TTCTGCTGGT CACTCAGCGC
    ATCACAAAAT ACCCTGTCTT GGTGGAAAGG ATATTGCAGT ACACAAAGGA GAGCACTGAA
    GAACAGAGAG ACTTATGCAA AGCTCTTTGC TTAATTAAAG ACATGATTGC AGCTGTGGAT
    TTAAAAGTCA GCGAATATGA GAAAAAACAA AAATGGCTTG AGATCCTAAA TAAGATCGAA
    AACAAAACAT ACACCAAGCT CAAAAATGGC CATGTGTTTA GGAAACAGGC ACTGATGAGT
    AAAGAGAGGA CCCTGTTATA TGATGGCCTT GTCTATTGGA AAACTGCTAC AGGTCGTTTC
    AAAGATATCC TAGCTCTACT TCTAACTGAT GTGCTGCTCT TTTTACAAGA AAAAGACCAG
    AAATACATCT TTGCAGCTGT TGATCAGAAG CCATCAGTTA TTTCCCTTCA GAAGCTCATT
    GCTAGGGAAG TTGCGAATGA GGAGAGAGGA ATGTTTTTGA TCAGCGCTTC ATCTGCTGGT
    CCCGAGATGT ATGAAATCCA TACCAACTCC AAGGAGGAAA GAAATACCTG GATGAGACGG
    ATCCAACAGG CAGTAGAAAG TTGCCCAGAA GAAGAAGGGG GAAGGACAAG TGAGTCTGAT
    GAAGAGAGGA GGAAAGCTGA AGCCAGAGTG GCGAAGATTC AGCAATGCCA AGAAATACTC
    AGTAACCAAG ATCAACAAAT TTGCACATAT TTGGAAGAGA AACTGCATAT CTACGCGAAA
    CTTGGAGAAC TAAGTGGATT TCAGGATGTC CATCTAGAGC CCCACCTCCT CATTCAACCA
    GACCCCGGAG AGCCTCCCCA GGCAGCCTCA TTACTGGCCG CAGCACTGAA AGAGGCTGAG
    AGTCTGCAGG TGGCAGTGAA GGCTTCCCAG ATGAGCGATG CATGCCAGTC CCCCGAGGAA
    GGTTGTGGAG AACCTGCTTT GCCGGACGTG CTCCATTCTC AGGACGCACC AGGATCGTCT
    ACTGCTTCAC TAGTCACAGA AGGGACAGGA GGAAGAGGCT GTTGGGATGT GGATGCCGGG
    ACCCAGGGTG TGGGAACCGA CCTGGCGGTT TCTGATGCGG GGGAGAAGGT GGAATATAGA
    AGTTTCCCAG GTTCTTTGCA GTCAGAGATT ATACAAGCGA TACAGAATTT AACTCGTCTC
    TTATATAGCC TTCAGGCCGC CTTGACCATT CAGGATAGCC ACATTGAGAT CCACAGGCTG
    GTTCTCCAAC AGCGGGAGAG CCTGGCCCCG GGCCCCGCGT TCCGAGGCAG CCCCTTTCAG
    GACCAGGAGA AGTCCCGCAG CCTGGAGAAG CAGCGGGAGG AGCTGGCCGA TACCCACAAG
    CTGCAGCTCC AGTTCCAGCA AGACCAGCAC CGCTGGCTCC GCAGGTGCGA CCAGCAGCAG
    CGCGAGCAGG AGGCCCGGGC CAGCCGGCTG CAGGAGCGCG AGAGGGAGTG CCGGGCGCAG
    GAGGAGCTGC TGCTCAGGAG CCGGGGCGAG CTGGACCAGC AGCTCCAAGA CTACCAGCAG
    AGCCTGGAGC GCCTGCGCGA GGGCCAGCGC CTGGTGGAGA GGGAGCGGGA GAGGATGCGG
    GCCCAGCAGA GCCTGCTGCG CCACTGGAAG CACGGGCGGC AGAGCAGCCT TCCCGCCGCG
    TTTTCTCCAG GAGCCCTGGA GGTACGGGAC TCCTGTGCTC GCAGAAGCAG AGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa39032
    Clone ID Related Accession (Same CDS sequence) NM_001102307.1
    Accession Version NM_001102307.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4170bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-13
    Organism Bos taurus(cattle)
    Product rho guanine nucleotide exchange factor 28
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC149175.1. On Aug 21, 2007 this sequence version replaced XR_028065.1. ##Evidence-Data-START## Transcript exon combination :: BC149175.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGATTCTG ACTCGGACTC ACCTTTTAAC TACTCGTGGC CTTCCTTCCC CAAAATGAAG 
    ATTCGACGGA GGGCGTCCAA ACAAGATCGT TCCTTTGATG CCCTCAAAAA ATCCAAGCAC
    CCCTCAACAT TGCTTGCTGC TGGCCAGCTC TCTGACATGC TGAATGGTGG TGATGACGTC
    TACGCTAACT GCATGGTGAT AGACCAGGTT GGTGATTTGG ACATTAACTA TATTAATATA
    GAGGAAATCC CTGCAAATAC CTGTCTTGAA TCCATGGATT CTTCTCTGGG GCCTGAGAGC
    AGCAAACCCA GCCTTCCTAC TGAAGCCAGT GCTGGCACAT ACCCACCAAA TAAGAGGCCA
    GAAGTGACGT CACACACGGA AGCCCAATCA TCTTTCATAT CATCTAGTTC GTATGCTTCC
    AATTTGAATC TTTCTTTTGG TCTTCGTGGA TTTGAAAAGG AGCAAAGTCG TCTAAAGAAA
    AGAAGTTCCA GCCTTGATGC CTTGGATGCT GACAGTGAAG GAGAGGGCCA TTCGGAGCAG
    TCGCACAATT GCTACACTCC CGTGTGTCAG AGTTCCTCGA GAACTGGCAT TGCTAGCGGG
    GATGAATTGG ACTCTTTTGA GACCAACACT GAACCAGATT TTAATATCTC CAGGACGGAA
    TCATTTTCTT TATCAAGTAG CCTGCAGCTG AAGGAATCAT TGTTTTCTGG AATCCGTTCA
    CGTTCGTATT CCTGCTCATC ACCCAAAATT TCTTTAGGAA AAACTCGATT GATGCGTGAT
    TTTACAGTGT GTAATTCAAT TGAAGAGCAA AGAGCTTACA GCTTGCCGGA GCCACCGAGA
    GAACAAAGAA TTCAGGAAGA AGAATGGGAT AAATATATCA TACCTGCCAA ATCAGAATCT
    GAAAAATACA AAGTAAGCCG AACTTTTAGC TTCCTCATGA ATAGGATGAC CAGCCCTCGG
    AATAAATCAA AGACAAAAGG CAAGGATGCC AAAGACAAAG AGAAACTGAC TCGACATCAG
    TTTGTCCCTG GAACTTTCTC TGGGGTTCTG CAGTGTTTGG TTTGCGATAA AACGCTCCTG
    GGGAAAGAGT CATTCCAGTG CTCCAACTGT AATGGAAACG TACATAAAGT CTGTAAAGAT
    GCTGCCCCTC CGTGTACCAA GAAATTCCAA GACAAATATA ATAAGAACAA ACCACAGACC
    ATCCTTGGAA ATTCTTCGTT TCGAGACGTC CCATTGCCTG GGCTCTCTCT GCACCCTTCC
    TCCTCCATGC CTATCGGGTT GCCAACTGGG AGGAAGGAGA TGGCAGGGCA AGTGCATCCC
    TTGTCCAGAA GTGTCCCAGG CACCACCTTG GAAAGCTTCA GGAGGCCGGG GGCATCCTTG
    GAGTCCGAGA ATGATGGCAG CACCTGGAGA AGCAGGTCTC ACTCTGACGA GCTGTTCCAG
    CCCATGGGCT CGTCCCCCTC CACGGACTCC TTCTTAACGG AAGATGTCGT GGATTCTTCT
    CTGTGGAGTG ACCTCAGCGG CGATGCCCAG GAGTTTGAAG CGGAATCTTG GAGTCTGGTG
    GTGGACCCTT CATTTTGTAG TCGGCAGGAG AAGGATGTCA TCCAAAGACA GGATGTCATT
    TTTGAGCTAA TGCAGACAGA ACTGCATCAC ATCCAGACCC TGTTCATCAT GTCTGAGATC
    TTCAGGAAAG GGATGAAAGA GGAGCTGCAG CTGGACCACA GCACTGTGGA CAAAATCTTC
    CCCTGTCTGG ACGAGTTACT TGAAATCCAC AAGCATTTCT TCTGTAGTAT GAAGGAGCGA
    AGGCAGGAAT CCTGTGTTGG CAATGACAGG AATTTCATCA TCGGCCGAAT TGGAGATATT
    CTAGTACAAC AGTTTTCAGA AGAAAATGCA AATAAAATGA AGAAAATATA TGGGGAATTC
    TGTAGCCATC ACAAAGAGGC TGTTAGCCTC TTTAAAGAAC TCCAGCAGAA TAAAAAGTTT
    CAGAATTTTA TTAAGCTCCG AAATAGTAAC CTTCTGGCTC GACGCCGAGG AATTCCAGAA
    TGCATTCTGC TGGTCACTCA GCGCATCACA AAATACCCTG TCTTGGTGGA AAGGATATTG
    CAGTACACAA AGGAGAGCAC TGAAGAACAG AGAGACTTAT GCAAAGCTCT TTGCTTAATT
    AAAGACATGA TTGCAGCTGT GGATTTAAAA GTCAGCGAAT ATGAGAAAAA ACAAAAATGG
    CTTGAGATCC TAAATAAGAT CGAAAACAAA ACATACACCA AGCTCAAAAA TGGCCATGTG
    TTTAGGAAAC AGGCACTGAT GAGTAAAGAG AGGACCCTGT TATATGATGG CCTTGTCTAT
    TGGAAAACTG CTACAGGTCG TTTCAAAGAT ATCCTAGCTC TACTTCTAAC TGATGTGCTG
    CTCTTTTTAC AAGAAAAAGA CCAGAAATAC ATCTTTGCAG CTGTTGATCA GAAGCCATCA
    GTTATTTCCC TTCAGAAGCT CATTGCTAGG GAAGTTGCGA ATGAGGAGAG AGGAATGTTT
    TTGATCAGCG CTTCATCTGC TGGTCCCGAG ATGTATGAAA TCCATACCAA CTCTAAGGAG
    GAAAGAAATA CCTGGATGAG ACGGATCCAA CAGGCAGTAG AAAGTTGCCC AGAAGAAGAA
    GGGGGAAGGA CAAGTGAGTC TGATGAAGAG AGGAGGAAAG CTGAAGCCAG AGTGGCGAAG
    ATTCAGCAAT GCCAAGAAAT ACTCAGTAAC CAAGATCAAC AAATTTGCAC ATATTTGGAA
    GAGAAACTGC ATATCTACGC GAAACTTGGA GAACTAAGTG GATTTCAGGA TGTCCATCTA
    GAGCCCCACC TCCTCATTCA ACCAGACCCC GGAGAGCCTC CCCAGGCAGC CTCATTACTG
    GCCGCAGCAC TGAAAGAGGC TGAGAGTCTG CAGGTGGCAG TGAAGGCTTC CCAGATGAGC
    GATGCATGCC AGTCCCCCGA GGAAGGTTGT GGAGAACCTG CTTTGCCGGA CGTGCTCCAT
    TCTCAGGACG CACCAGGATC GTCTACTGCT TCACTAGTCA CAGAAGGGAC AGGAGGAAGA
    GGCTGTTGGG ATGTGGATGC CGGGACCCAG GGTGTGGGAA CCGACCTGGC GGTTTCTGAT
    GCGGGGGAGA AGGTGGAATA TAGAAGTTTC CCAGGTTCTT TGCAGTCAGA GATTATACAA
    GCGATACAGA ATTTAACTCG TCTCTTATAT AGCCTTCAGG CCGCCTTGAC CATTCAGGAT
    AGCCACATTG AGATCCACAG GCTGGTTCTC CAACAGCGGG AGAGCCTGGC CCCGGGCCCC
    GCGTTCCGAG GCAGCCCCTT TCAGGACCAG GAGAAGTCCC GCAGCCTGGA GAAGCAGCGG
    GAGGAGCTGG CCGATACCCA CAAGCTGCAG CTCCAGTTCC AGCAAGACCA GCACCGCTGG
    CTCCGCAGGT GCGACCAGCA GCAGCGCGAG CAGGAGGCCC GGGCCAGCCG GCTGCAGGAG
    CGCGAGAGGG AGTGCCGGGC GCAGGAGGAG CTGCTGCTCA GGAGCCGGGG CGAGCTGGAC
    CAGCAGCTCC AAGACTACCA GCAGAGCCTG GAGCGCCTGC GCGAGGGCCA GCGCCTGGTG
    GAGAGGGAGC GGGAGAGGAT GCGGGCCCAG CAGAGCCTGC TGCGCCACTG GAAGCACGGG
    CGGCAGAGCA GCCTTCCCGC CGCGTTTTCT CCAGGAGCCC TGGAGGTAAT GGAACTTAAT
    CGATCTGAGA CTTTACGTCA TGAAAATTCA TTCTTCATCA ATGAAGCTTT AGTGCAAATG
    TCACTCAACC CTTTGAACAA ACCGCCGCCA CCAGGTATCC ATCAGGATGC GGCTTACCCC
    CCAGGCATAC CTCATTCTGA TTTGGTAAGG ACTACTGAAA ATCAAGTAGA CCTCAAGACG
    GACGTTTCTC AACCCTCAGA TATCAACCAT GAACTGTGGA TAGCGGCTGG GGCCTGTCAT
    CAGATACCCC CTCTCCACCA AAGCAGCAAG GATTCTTGTA AAAATGATGT GAACATCTCT
    CAGACTGCAT ACCCACCCCT CCATGACTCA AATCCACACA GCCCTCGGCT GCAGACGTTT
    ATAGCAGAAG AAAAGCTAAA TCTACAGAGA GTGACCAGAC AAGACGGGGA AACTGGAAAT
    GGAGCAGAAG AAAATATTGT TTACCTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001095777.2
    CDS21..4190
    Translation

    Target ORF information:

    RefSeq Version NM_001102307.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Rho guanine nucleotide exchange factor 28 (ARHGEF28), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001102307.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGATTCTG ACTCGGACTC ACCTTTTAAC TACTCGTGGC CTTCCTTCCC CAAAATGAAG 
    ATTCGACGGA GGGCGTCCAA ACAAGATCGT TCCTTTGATG CCCTCAAAAA ATCCAAGCAC
    CCCTCAACAT TGCTTGCTGC TGGCCAGCTC TCTGACATGC TGAATGGTGG TGATGACGTC
    TACGCTAACT GCATGGTGAT AGACCAGGTT GGTGATTTGG ACATTAACTA TATTAATATA
    GAGGAAATCC CTGCAAATAC CTGTCTTGAA TCCATGGATT CTTCTCTGGG GCCTGAGAGC
    AGCAAACCCA GCCTTCCTAC TGAAGCCAGT GCTGGCACAT ACCCACCAAA TAAGAGGCCA
    GAAGTGACGT CACACACGGA AGCCCAATCA TCTTTCATAT CATCTAGTTC GTATGCTTCC
    AATTTGAATC TTTCTTTTGG TCTTCGTGGA TTTGAAAAGG AGCAAAGTCG TCTAAAGAAA
    AGAAGTTCCA GCCTTGATGC CTTGGATGCT GACAGTGAAG GAGAGGGCCA TTCGGAGCAG
    TCGCACAATT GCTACACTCC CGTGTGTCAG AGTTCCTCGA GAACTGGCAT TGCTAGCGGG
    GATGAATTGG ACTCTTTTGA GACCAACACT GAACCAGATT TTAATATCTC CAGGACGGAA
    TCATTTTCTT TATCAAGTAG CCTGCAGCTG AAGGAATCAT TGTTTTCTGG AATCCGTTCA
    CGTTCGTATT CCTGCTCATC ACCCAAAATT TCTTTAGGAA AAACTCGATT GATGCGTGAT
    TTTACAGTGT GTAATTCAAT TGAAGAGCAA AGAGCTTACA GCTTGCCGGA GCCACCGAGA
    GAACAAAGAA TTCAGGAAGA AGAATGGGAT AAATATATCA TACCTGCCAA ATCAGAATCT
    GAAAAATACA AAGTAAGCCG AACTTTTAGC TTCCTCATGA ATAGGATGAC CAGCCCTCGG
    AATAAATCAA AGACAAAAGG CAAGGATGCC AAAGACAAAG AGAAACTGAC TCGACATCAG
    TTTGTCCCTG GAACTTTCTC TGGGGTTCTG CAGTGTTTGG TTTGCGATAA AACGCTCCTG
    GGGAAAGAGT CATTCCAGTG CTCCAACTGT AATGGAAACG TACATAAAGT CTGTAAAGAT
    GCTGCCCCTC CGTGTACCAA GAAATTCCAA GACAAATATA ATAAGAACAA ACCACAGACC
    ATCCTTGGAA ATTCTTCGTT TCGAGACGTC CCATTGCCTG GGCTCTCTCT GCACCCTTCC
    TCCTCCATGC CTATCGGGTT GCCAACTGGG AGGAAGGAGA TGGCAGGGCA AGTGCATCCC
    TTGTCCAGAA GTGTCCCAGG CACCACCTTG GAAAGCTTCA GGAGGCCGGG GGCATCCTTG
    GAGTCCGAGA ATGATGGCAG CACCTGGAGA AGCAGGTCTC ACTCTGACGA GCTGTTCCAG
    CCCATGGGCT CGTCCCCCTC CACGGACTCC TTCTTAACGG AAGATGTCGT GGATTCTTCT
    CTGTGGAGTG ACCTCAGCGG CGATGCCCAG GAGTTTGAAG CGGAATCTTG GAGTCTGGTG
    GTGGACCCTT CATTTTGTAG TCGGCAGGAG AAGGATGTCA TCCAAAGACA GGATGTCATT
    TTTGAGCTAA TGCAGACAGA ACTGCATCAC ATCCAGACCC TGTTCATCAT GTCTGAGATC
    TTCAGGAAAG GGATGAAAGA GGAGCTGCAG CTGGACCACA GCACTGTGGA CAAAATCTTC
    CCCTGTCTGG ACGAGTTACT TGAAATCCAC AAGCATTTCT TCTGTAGTAT GAAGGAGCGA
    AGGCAGGAAT CCTGTGTTGG CAATGACAGG AATTTCATCA TCGGCCGAAT TGGAGATATT
    CTAGTACAAC AGTTTTCAGA AGAAAATGCA AATAAAATGA AGAAAATATA TGGGGAATTC
    TGTAGCCATC ACAAAGAGGC TGTTAGCCTC TTTAAAGAAC TCCAGCAGAA TAAAAAGTTT
    CAGAATTTTA TTAAGCTCCG AAATAGTAAC CTTCTGGCTC GACGCCGAGG AATTCCAGAA
    TGCATTCTGC TGGTCACTCA GCGCATCACA AAATACCCTG TCTTGGTGGA AAGGATATTG
    CAGTACACAA AGGAGAGCAC TGAAGAACAG AGAGACTTAT GCAAAGCTCT TTGCTTAATT
    AAAGACATGA TTGCAGCTGT GGATTTAAAA GTCAGCGAAT ATGAGAAAAA ACAAAAATGG
    CTTGAGATCC TAAATAAGAT CGAAAACAAA ACATACACCA AGCTCAAAAA TGGCCATGTG
    TTTAGGAAAC AGGCACTGAT GAGTAAAGAG AGGACCCTGT TATATGATGG CCTTGTCTAT
    TGGAAAACTG CTACAGGTCG TTTCAAAGAT ATCCTAGCTC TACTTCTAAC TGATGTGCTG
    CTCTTTTTAC AAGAAAAAGA CCAGAAATAC ATCTTTGCAG CTGTTGATCA GAAGCCATCA
    GTTATTTCCC TTCAGAAGCT CATTGCTAGG GAAGTTGCGA ATGAGGAGAG AGGAATGTTT
    TTGATCAGCG CTTCATCTGC TGGTCCCGAG ATGTATGAAA TCCATACCAA CTCTAAGGAG
    GAAAGAAATA CCTGGATGAG ACGGATCCAA CAGGCAGTAG AAAGTTGCCC AGAAGAAGAA
    GGGGGAAGGA CAAGTGAGTC TGATGAAGAG AGGAGGAAAG CTGAAGCCAG AGTGGCGAAG
    ATTCAGCAAT GCCAAGAAAT ACTCAGTAAC CAAGATCAAC AAATTTGCAC ATATTTGGAA
    GAGAAACTGC ATATCTACGC GAAACTTGGA GAACTAAGTG GATTTCAGGA TGTCCATCTA
    GAGCCCCACC TCCTCATTCA ACCAGACCCC GGAGAGCCTC CCCAGGCAGC CTCATTACTG
    GCCGCAGCAC TGAAAGAGGC TGAGAGTCTG CAGGTGGCAG TGAAGGCTTC CCAGATGAGC
    GATGCATGCC AGTCCCCCGA GGAAGGTTGT GGAGAACCTG CTTTGCCGGA CGTGCTCCAT
    TCTCAGGACG CACCAGGATC GTCTACTGCT TCACTAGTCA CAGAAGGGAC AGGAGGAAGA
    GGCTGTTGGG ATGTGGATGC CGGGACCCAG GGTGTGGGAA CCGACCTGGC GGTTTCTGAT
    GCGGGGGAGA AGGTGGAATA TAGAAGTTTC CCAGGTTCTT TGCAGTCAGA GATTATACAA
    GCGATACAGA ATTTAACTCG TCTCTTATAT AGCCTTCAGG CCGCCTTGAC CATTCAGGAT
    AGCCACATTG AGATCCACAG GCTGGTTCTC CAACAGCGGG AGAGCCTGGC CCCGGGCCCC
    GCGTTCCGAG GCAGCCCCTT TCAGGACCAG GAGAAGTCCC GCAGCCTGGA GAAGCAGCGG
    GAGGAGCTGG CCGATACCCA CAAGCTGCAG CTCCAGTTCC AGCAAGACCA GCACCGCTGG
    CTCCGCAGGT GCGACCAGCA GCAGCGCGAG CAGGAGGCCC GGGCCAGCCG GCTGCAGGAG
    CGCGAGAGGG AGTGCCGGGC GCAGGAGGAG CTGCTGCTCA GGAGCCGGGG CGAGCTGGAC
    CAGCAGCTCC AAGACTACCA GCAGAGCCTG GAGCGCCTGC GCGAGGGCCA GCGCCTGGTG
    GAGAGGGAGC GGGAGAGGAT GCGGGCCCAG CAGAGCCTGC TGCGCCACTG GAAGCACGGG
    CGGCAGAGCA GCCTTCCCGC CGCGTTTTCT CCAGGAGCCC TGGAGGTAAT GGAACTTAAT
    CGATCTGAGA CTTTACGTCA TGAAAATTCA TTCTTCATCA ATGAAGCTTT AGTGCAAATG
    TCACTCAACC CTTTGAACAA ACCGCCGCCA CCAGGTATCC ATCAGGATGC GGCTTACCCC
    CCAGGCATAC CTCATTCTGA TTTGGTAAGG ACTACTGAAA ATCAAGTAGA CCTCAAGACG
    GACGTTTCTC AACCCTCAGA TATCAACCAT GAACTGTGGA TAGCGGCTGG GGCCTGTCAT
    CAGATACCCC CTCTCCACCA AAGCAGCAAG GATTCTTGTA AAAATGATGT GAACATCTCT
    CAGACTGCAT ACCCACCCCT CCATGACTCA AATCCACACA GCCCTCGGCT GCAGACGTTT
    ATAGCAGAAG AAAAGCTAAA TCTACAGAGA GTGACCAGAC AAGACGGGGA AACTGGAAAT
    GGAGCAGAAG AAAATATTGT TTACCTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.