CHFR cDNA ORF clone, Bos taurus(Cattle)

The following CHFR gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CHFR cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa27764 NM_001205474.2
Latest version!
Bos taurus checkpoint with forkhead and ring finger domains (CHFR), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa36751 NM_001205474.1
Latest version!
Bos taurus checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase (CHFR), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa27764 XM_005217679.3
Latest version!
Bos taurus checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase (CHFR), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa224391 NM_001205474.3
Latest version!
Bos taurus checkpoint with forkhead and ring finger domains (CHFR), mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OBa224391 XM_005217679.4
Latest version!
Bos taurus checkpoint with forkhead and ring finger domains (CHFR), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OBa224392 XM_024977446.1
Latest version!
Bos taurus checkpoint with forkhead and ring finger domains (CHFR), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa27764
    Clone ID Related Accession (Same CDS sequence) NM_001205474.2 , XM_005217679.3
    Accession Version NM_001205474.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1992bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-22
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase CHFR
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DAAA02044944.1. On Jun 30, 2017 this sequence version replaced NM_001205474.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN02822088, SAMN02822089 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGAGCGGC CGGGGGAAGG CGAGCAGCCG CCCCAGCAGC AGCCGTGGGG AAGGCTTCTT 
    CGCCTGGGCG CCGAGGAGGG CGAGCCGCAC GTCCTCCTGA GGAAACGGGA GTGGACCATC
    GGGCGAAGAA GAGGTTGTGA CCTTTCATTC CCTGGCAATA AATTGGTCTC TGGAGATCAC
    TGTAAAATTA CAGTGGATGA AAAATCGGGT CAGGTATCAC TGGAAGATAC CAGTGCCAAT
    GGAACTGTCA TTAACAAGCT GAAGGTTGTT AAGAAGCAAA CTTGCCCTTT ACAGACTGGG
    GATGTCATCT ACTTGGTGTA CAGGAAGAAT GAGCCAGAAC ACAACGTGGC ATACCTCTAC
    GAATCTCTAA ATGAAAAGCA AGGTGTAACA CAAGACTCCT TTGAAGCTAA TAAAGAAAAT
    GTGTTCCACG TGACCAAAGA TACCTCAGGT GCAGGGCGAG GTGACGATCC TCAGGATCCC
    CAGGTCCTGC TGTCGTCGCC CGCCACTCAG GTGTGCTTTG AGGAACCACA GCCATCGACG
    TCCACATCGG ACCTCTTCCC CACGGCCTCT ACCTCTTCCA TGGAGCCCAC CTCCGCAGGC
    CGAGGGCCTC CCTCCAGCTC CAGCTCCAGA GGTACAGACA TCTCTCCAAA GGGGTGTGGT
    CCATCTGTGA CAAGTGATGA AATCTCCAGC TTCCCTTTAA CTCTCCCAGA CAGAGAGGGG
    GCTTCCTTTT CTCTGTCGGA ACCCCAGGAT CAGGAGGATT TGGAGCCTGT TAAGAAGAGA
    GTAAAAGCAG TTGGGGAGCC AGACCTTAAC CTGCCCATAC TGGTCACAGA CCCAGGCAGA
    GACCCTCCCG CTGCCCTTGA CGTCAGAGCC GAGGCCATGA AGCCAGACAA GATGGAGGAG
    ACGCTCACGT GCATCATCTG CCAGGACCTG CTGCACGACT GTGTGAGCCT GCAGCCCTGT
    ATGCACACCT TCTGCGCTGC CTGCTACTCG GGCTGGATGG AGCGGTCTGC CCTGTGCCCC
    ACCTGCCGCT GTCCGGTTGA GCGCATCTGT AAAAACCACA TCCTCAACAA CCTGGTGGAG
    GCGTACCTGC TGCAGCACCC GGACAAGAGG CGCAGCGAGG AGGATCTGCG CAGCATGGCC
    GCCAGGAACA AGATCACACA GGACATGCTG CAGCCCAAGG TCAGGAGGGC CTTCTCCGAC
    GAGGAGGGCA GCTCTGAGGA CCTGCTGGAG CTGTCGGACG TGGACAGTGA GTCCTCGGAC
    GTTAGCCAGC CGTCCATCGT GTGCAGACAG TGCCCTGAGT ACCGCAGGCA GGCGGGGCAG
    CCCCTTCCCT ACCCAGGGCC CGGCAGCGAG CCAGGGGCAC CGCAGGTCCC CGGGGATGCA
    CCGTCCACGT CCGCCAGTGT CACGGCAGCC CAGGACTACG TGTGCGCCCT GCAAGGAAGC
    CACGCCATAT GCACCTGCTG CTTCCAGCCC ATGCCGGACC GGAGAGCGGA GCGTGAGCGG
    GACCCCCGCA TCGCCCCCCA GCAGTGTGCC ATCTGCCTGC AGCCCTTCTG CCACCTATAC
    TGGGGCTGTG CCCGGACTGG CTGCCTCGGC TGCCTGGCCC CGTTCTGCGA GCTCAACCTG
    GGTGACCGGT GTCTGGACGG GGTGCTGAGC AACAACAACT ATGAGTCGGA CGTCCTGAAG
    AATTACCTGG CCACCAGGGG TTTGACTTGG AAAAACATGT TGACTGAGAG CCTCGGGGCT
    CTGCAGAGGG GAGTATTCCT GCTGTCTGAT TACAGAATCA CCGGGAACAC CGTGCTGTGC
    TACTGCTGTG GCCTGCGGAG CTTCCGCGAG CTCACCTATC AGTATCGGCA GAACATTCCT
    GCTTCTGAGC TACCAGCGGC CGTCACGTCC CGTCCTGATT GCTACTGGGG CCGCAACTGC
    CGCACTCAGG TGAAGGCCCA CCACGCAATG AAATTCAATC ACATCTGTGA ACAGACGAGG
    TTCAAGAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001192403.1
    CDS1..1992
    Translation

    Target ORF information:

    RefSeq Version NM_001205474.2
    Organism Bos taurus(cattle)
    Definition Bos taurus checkpoint with forkhead and ring finger domains (CHFR), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001205474.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGAGCGGC CGGGGGAAGG CGAGCAGCCG CCCCAGCAGC AGCCGTGGGG AAGGCTTCTT 
    CGCCTGGGCG CCGAGGAGGG CGAGCCGCAC GTCCTCCTGA GGAAACGGGA GTGGACCATC
    GGGCGAAGAA GAGGTTGTGA CCTTTCATTC CCTGGCAATA AATTGGTCTC TGGAGATCAC
    TGTAAAATTA CAGTGGATGA AAAATCGGGT CAGGTATCAC TGGAAGATAC CAGTGCCAAT
    GGAACTGTCA TTAACAAGCT GAAGGTTGTT AAGAAGCAAA CTTGCCCTTT ACAGACTGGG
    GATGTCATCT ACTTGGTGTA CAGGAAGAAT GAGCCAGAAC ACAACGTGGC ATACCTCTAC
    GAATCTCTAA ATGAAAAGCA AGGTGTAACA CAAGACTCCT TTGAAGCTAA TAAAGAAAAT
    GTGTTCCACG TGACCAAAGA TACCTCAGGT GCAGGGCGAG GTGACGATCC TCAGGATCCC
    CAGGTCCTGC TGTCGTCGCC CGCCACTCAG GTGTGCTTTG AGGAACCACA GCCATCGACG
    TCCACATCGG ACCTCTTCCC CACGGCCTCT ACCTCTTCCA TGGAGCCCAC CTCCGCAGGC
    CGAGGGCCTC CCTCCAGCTC CAGCTCCAGA GGTACAGACA TCTCTCCAAA GGGGTGTGGT
    CCATCTGTGA CAAGTGATGA AATCTCCAGC TTCCCTTTAA CTCTCCCAGA CAGAGAGGGG
    GCTTCCTTTT CTCTGTCGGA ACCCCAGGAT CAGGAGGATT TGGAGCCTGT TAAGAAGAGA
    GTAAAAGCAG TTGGGGAGCC AGACCTTAAC CTGCCCATAC TGGTCACAGA CCCAGGCAGA
    GACCCTCCCG CTGCCCTTGA CGTCAGAGCC GAGGCCATGA AGCCAGACAA GATGGAGGAG
    ACGCTCACGT GCATCATCTG CCAGGACCTG CTGCACGACT GTGTGAGCCT GCAGCCCTGT
    ATGCACACCT TCTGCGCTGC CTGCTACTCG GGCTGGATGG AGCGGTCTGC CCTGTGCCCC
    ACCTGCCGCT GTCCGGTTGA GCGCATCTGT AAAAACCACA TCCTCAACAA CCTGGTGGAG
    GCGTACCTGC TGCAGCACCC GGACAAGAGG CGCAGCGAGG AGGATCTGCG CAGCATGGCC
    GCCAGGAACA AGATCACACA GGACATGCTG CAGCCCAAGG TCAGGAGGGC CTTCTCCGAC
    GAGGAGGGCA GCTCTGAGGA CCTGCTGGAG CTGTCGGACG TGGACAGTGA GTCCTCGGAC
    GTTAGCCAGC CGTCCATCGT GTGCAGACAG TGCCCTGAGT ACCGCAGGCA GGCGGGGCAG
    CCCCTTCCCT ACCCAGGGCC CGGCAGCGAG CCAGGGGCAC CGCAGGTCCC CGGGGATGCA
    CCGTCCACGT CCGCCAGTGT CACGGCAGCC CAGGACTACG TGTGCGCCCT GCAAGGAAGC
    CACGCCATAT GCACCTGCTG CTTCCAGCCC ATGCCGGACC GGAGAGCGGA GCGTGAGCGG
    GACCCCCGCA TCGCCCCCCA GCAGTGTGCC ATCTGCCTGC AGCCCTTCTG CCACCTATAC
    TGGGGCTGTG CCCGGACTGG CTGCCTCGGC TGCCTGGCCC CGTTCTGCGA GCTCAACCTG
    GGTGACCGGT GTCTGGACGG GGTGCTGAGC AACAACAACT ATGAGTCGGA CGTCCTGAAG
    AATTACCTGG CCACCAGGGG TTTGACTTGG AAAAACATGT TGACTGAGAG CCTCGGGGCT
    CTGCAGAGGG GAGTATTCCT GCTGTCTGAT TACAGAATCA CCGGGAACAC CGTGCTGTGC
    TACTGCTGTG GCCTGCGGAG CTTCCGCGAG CTCACCTATC AGTATCGGCA GAACATTCCT
    GCTTCTGAGC TACCAGCGGC CGTCACGTCC CGTCCTGATT GCTACTGGGG CCGCAACTGC
    CGCACTCAGG TGAAGGCCCA CCACGCAATG AAATTCAATC ACATCTGTGA ACAGACGAGG
    TTCAAGAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa36751
    Clone ID Related Accession (Same CDS sequence) NM_001205474.1
    Accession Version NM_001205474.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1992bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-01-03
    Organism Bos taurus(Cattle)
    Product E3 ubiquitin-protein ligase CHFR
    Comment PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAFC03091943.1. On Apr 20, 2011 this sequence version replaced XM_001788064.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00000728, SAMN01093727 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGAGCGGC CGGGGGAAGG CGAGCAGCCG CCCCAGCAGC AGCCGTGGGG AAGGCTTCTT 
    CGCCTGGGCG CCGAGGAGGG CGAGCCGCAC GTCCTCCTGA GGAAACGGGA GTGGACCATC
    GGGCGAAGAA GAGGTTGTGA CCTTTCATTC CCTGGCAATA AATTGGTCTC TGGAGATCAC
    TGTAAAATTA CAGTGGATGA AAAATCGGGT CAGGTATCAC TGGAAGATAC CAGTGCCAAT
    GGAACTGTCA TTAACAAGCT GAAGGTTGTT AAGAAGCAAA CTTGCCCTTT ACAGACTGGG
    GATGTCATCT ACTTGGTGTA CAGGAAGAAT GAGCCAGAAC ACAACGTGGC ATACCTCTAC
    GAATCTCTAA ATGAAAAGCA AGGTGTAACA CAAGACTCCT TTGAAGCTAA TAAAGAAAAT
    GTGTTCCACG TGACCAAAGA TACCTCAGGT GCAGGGCGAG GTGACGATCC TCAGGATCCC
    CAGGTCCTGC TGTCGTCGCC CGCCACTCAG GTGTGCTTTG AGGAACCACA GCCATCGACG
    TCCACATCGG ACCTCTTCCC CACGGCCTCT ACCTCTTCCA TGGAGCCCAC CTCCGCAGGC
    CGAGGGCCTC CCTCCAGCTC CAGCTCCAGA GGTACAGACA TCTCTCCAAA GGGGTGTGGT
    CCATCTGTGA CAAGTGATGA AATCTCCAGC TTCCCTTTAA CTCTCCCAGA CAGAGAGGGG
    GCTTCCTTTT CTCTGTCGGA ACCCCAGGAT CAGGAGGATT TGGAGCCTGT TAAGAAGAGA
    GTAAAAGCAG TTGGGGAGCC AGACCTTAAC CTGCCCATAC TGGTCACAGA CCCAGGCAGA
    GACCCTCCCG CTGCCCTTGA CGTCAGAGCC GAGGCCATGA AGCCAGACAA GATGGAGGAG
    ACGCTCACGT GCATCATCTG CCAGGACCTG CTGCACGACT GTGTGAGCCT GCAGCCCTGT
    ATGCACACCT TCTGCGCTGC CTGCTACTCG GGCTGGATGG AGCGGTCTGC CCTGTGCCCC
    ACCTGCCGCT GTCCGGTTGA GCGCATCTGT AAAAACCACA TCCTCAACAA CCTGGTGGAG
    GCGTACCTGC TGCAGCACCC GGACAAGAGG CGCAGCGAGG AGGATCTGCG CAGCATGGCC
    GCCAGGAACA AGATCACACA GGACATGCTG CAGCCCAAGG TCAGGAGGGC CTTCTCCGAC
    GAGGAGGGCA GCTCTGAGGA CCTGCTGGAG CTGTCGGACG TGGACAGTGA GTCCTCGGAC
    GTTAGCCAGC CGTCCATCGT GTGCAGACAG TGCCCTGAGT ACCGCAGGCA GGCGGGGCAG
    CCCCTTCCCT ACCCAGGGCC CGGCAGCGAG CCAGGGGCAC CGCAGGTCCC CGGGGATGCA
    CCGTCCACGT CCGCCAGTGT CACGGCAGCC CAGGACTACG TGTGCGCCCT TCAAGGAAGC
    CACGCCATAT GCACCTGCTG CTTCCAGCCC ATGCCGGACC GGAGAGCGGA GCGTGAGCGG
    GACCCCCGCA TCGCCCCCCA GCAGTGTGCC ATCTGCCTGC AGCCCTTCTG CCACCTATAC
    TGGGGCTGTG CCCGGACTGG CTGCCTCGGC TGCCTGGCCC CGTTCTGCGA GCTCAACCTG
    GGTGACCGGT GTCTGGACGG GGTGCTGAGC AACAACAACT ATGAGTCGGA CGTCCTGAAG
    AATTACCTGG CCACCAGGGG TTTGACTTGG AAAAACATGT TGACTGAGAG CCTCGGGGCT
    CTGCAGAGGG GAGTATTCCT GCTGTCTGAT TACAGAATCA CCGGGAACAC CGTGCTGTGC
    TACTGCTGTG GCCTGCGGAG CTTCCGCGAG CTCACCTACC AGTATCGGCA GAACATTCCT
    GCTTCTGAGC TACCAGCGGC CGTCACGTCC CGTCCTGATT GCTACTGGGG CCGCAACTGC
    CGCACTCAGG TGAAGGCCCA CCACGCAATG AAATTCAATC ACATCTGTGA ACAGACGAGG
    TTCAAGAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001192403.1
    CDS1..1992
    Translation

    Target ORF information:

    RefSeq Version NM_001205474.1
    Organism Bos taurus(Cattle)
    Definition Bos taurus checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase (CHFR), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001205474.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGAGCGGC CGGGGGAAGG CGAGCAGCCG CCCCAGCAGC AGCCGTGGGG AAGGCTTCTT 
    CGCCTGGGCG CCGAGGAGGG CGAGCCGCAC GTCCTCCTGA GGAAACGGGA GTGGACCATC
    GGGCGAAGAA GAGGTTGTGA CCTTTCATTC CCTGGCAATA AATTGGTCTC TGGAGATCAC
    TGTAAAATTA CAGTGGATGA AAAATCGGGT CAGGTATCAC TGGAAGATAC CAGTGCCAAT
    GGAACTGTCA TTAACAAGCT GAAGGTTGTT AAGAAGCAAA CTTGCCCTTT ACAGACTGGG
    GATGTCATCT ACTTGGTGTA CAGGAAGAAT GAGCCAGAAC ACAACGTGGC ATACCTCTAC
    GAATCTCTAA ATGAAAAGCA AGGTGTAACA CAAGACTCCT TTGAAGCTAA TAAAGAAAAT
    GTGTTCCACG TGACCAAAGA TACCTCAGGT GCAGGGCGAG GTGACGATCC TCAGGATCCC
    CAGGTCCTGC TGTCGTCGCC CGCCACTCAG GTGTGCTTTG AGGAACCACA GCCATCGACG
    TCCACATCGG ACCTCTTCCC CACGGCCTCT ACCTCTTCCA TGGAGCCCAC CTCCGCAGGC
    CGAGGGCCTC CCTCCAGCTC CAGCTCCAGA GGTACAGACA TCTCTCCAAA GGGGTGTGGT
    CCATCTGTGA CAAGTGATGA AATCTCCAGC TTCCCTTTAA CTCTCCCAGA CAGAGAGGGG
    GCTTCCTTTT CTCTGTCGGA ACCCCAGGAT CAGGAGGATT TGGAGCCTGT TAAGAAGAGA
    GTAAAAGCAG TTGGGGAGCC AGACCTTAAC CTGCCCATAC TGGTCACAGA CCCAGGCAGA
    GACCCTCCCG CTGCCCTTGA CGTCAGAGCC GAGGCCATGA AGCCAGACAA GATGGAGGAG
    ACGCTCACGT GCATCATCTG CCAGGACCTG CTGCACGACT GTGTGAGCCT GCAGCCCTGT
    ATGCACACCT TCTGCGCTGC CTGCTACTCG GGCTGGATGG AGCGGTCTGC CCTGTGCCCC
    ACCTGCCGCT GTCCGGTTGA GCGCATCTGT AAAAACCACA TCCTCAACAA CCTGGTGGAG
    GCGTACCTGC TGCAGCACCC GGACAAGAGG CGCAGCGAGG AGGATCTGCG CAGCATGGCC
    GCCAGGAACA AGATCACACA GGACATGCTG CAGCCCAAGG TCAGGAGGGC CTTCTCCGAC
    GAGGAGGGCA GCTCTGAGGA CCTGCTGGAG CTGTCGGACG TGGACAGTGA GTCCTCGGAC
    GTTAGCCAGC CGTCCATCGT GTGCAGACAG TGCCCTGAGT ACCGCAGGCA GGCGGGGCAG
    CCCCTTCCCT ACCCAGGGCC CGGCAGCGAG CCAGGGGCAC CGCAGGTCCC CGGGGATGCA
    CCGTCCACGT CCGCCAGTGT CACGGCAGCC CAGGACTACG TGTGCGCCCT TCAAGGAAGC
    CACGCCATAT GCACCTGCTG CTTCCAGCCC ATGCCGGACC GGAGAGCGGA GCGTGAGCGG
    GACCCCCGCA TCGCCCCCCA GCAGTGTGCC ATCTGCCTGC AGCCCTTCTG CCACCTATAC
    TGGGGCTGTG CCCGGACTGG CTGCCTCGGC TGCCTGGCCC CGTTCTGCGA GCTCAACCTG
    GGTGACCGGT GTCTGGACGG GGTGCTGAGC AACAACAACT ATGAGTCGGA CGTCCTGAAG
    AATTACCTGG CCACCAGGGG TTTGACTTGG AAAAACATGT TGACTGAGAG CCTCGGGGCT
    CTGCAGAGGG GAGTATTCCT GCTGTCTGAT TACAGAATCA CCGGGAACAC CGTGCTGTGC
    TACTGCTGTG GCCTGCGGAG CTTCCGCGAG CTCACCTACC AGTATCGGCA GAACATTCCT
    GCTTCTGAGC TACCAGCGGC CGTCACGTCC CGTCCTGATT GCTACTGGGG CCGCAACTGC
    CGCACTCAGG TGAAGGCCCA CCACGCAATG AAATTCAATC ACATCTGTGA ACAGACGAGG
    TTCAAGAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa27764
    Clone ID Related Accession (Same CDS sequence) NM_001205474.2 , XM_005217679.3
    Accession Version XM_005217679.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1992bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase CHFR isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104452.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005217679.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGAGCGGC CGGGGGAAGG CGAGCAGCCG CCCCAGCAGC AGCCGTGGGG AAGGCTTCTT 
    CGCCTGGGCG CCGAGGAGGG CGAGCCGCAC GTCCTCCTGA GGAAACGGGA GTGGACCATC
    GGGCGAAGAA GAGGTTGTGA CCTTTCATTC CCTGGCAATA AATTGGTCTC TGGAGATCAC
    TGTAAAATTA CAGTGGATGA AAAATCGGGT CAGGTATCAC TGGAAGATAC CAGTGCCAAT
    GGAACTGTCA TTAACAAGCT GAAGGTTGTT AAGAAGCAAA CTTGCCCTTT ACAGACTGGG
    GATGTCATCT ACTTGGTGTA CAGGAAGAAT GAGCCAGAAC ACAACGTGGC ATACCTCTAC
    GAATCTCTAA ATGAAAAGCA AGGTGTAACA CAAGACTCCT TTGAAGCTAA TAAAGAAAAT
    GTGTTCCACG TGACCAAAGA TACCTCAGGT GCAGGGCGAG GTGACGATCC TCAGGATCCC
    CAGGTCCTGC TGTCGTCGCC CGCCACTCAG GTGTGCTTTG AGGAACCACA GCCATCGACG
    TCCACATCGG ACCTCTTCCC CACGGCCTCT ACCTCTTCCA TGGAGCCCAC CTCCGCAGGC
    CGAGGGCCTC CCTCCAGCTC CAGCTCCAGA GGTACAGACA TCTCTCCAAA GGGGTGTGGT
    CCATCTGTGA CAAGTGATGA AATCTCCAGC TTCCCTTTAA CTCTCCCAGA CAGAGAGGGG
    GCTTCCTTTT CTCTGTCGGA ACCCCAGGAT CAGGAGGATT TGGAGCCTGT TAAGAAGAGA
    GTAAAAGCAG TTGGGGAGCC AGACCTTAAC CTGCCCATAC TGGTCACAGA CCCAGGCAGA
    GACCCTCCCG CTGCCCTTGA CGTCAGAGCC GAGGCCATGA AGCCAGACAA GATGGAGGAG
    ACGCTCACGT GCATCATCTG CCAGGACCTG CTGCACGACT GTGTGAGCCT GCAGCCCTGT
    ATGCACACCT TCTGCGCTGC CTGCTACTCG GGCTGGATGG AGCGGTCTGC CCTGTGCCCC
    ACCTGCCGCT GTCCGGTTGA GCGCATCTGT AAAAACCACA TCCTCAACAA CCTGGTGGAG
    GCGTACCTGC TGCAGCACCC GGACAAGAGG CGCAGCGAGG AGGATCTGCG CAGCATGGCC
    GCCAGGAACA AGATCACACA GGACATGCTG CAGCCCAAGG TCAGGAGGGC CTTCTCCGAC
    GAGGAGGGCA GCTCTGAGGA CCTGCTGGAG CTGTCGGACG TGGACAGTGA GTCCTCGGAC
    GTTAGCCAGC CGTCCATCGT GTGCAGACAG TGCCCTGAGT ACCGCAGGCA GGCGGGGCAG
    CCCCTTCCCT ACCCAGGGCC CGGCAGCGAG CCAGGGGCAC CGCAGGTCCC CGGGGATGCA
    CCGTCCACGT CCGCCAGTGT CACGGCAGCC CAGGACTACG TGTGCGCCCT GCAAGGAAGC
    CACGCCATAT GCACCTGCTG CTTCCAGCCC ATGCCGGACC GGAGAGCGGA GCGTGAGCGG
    GACCCCCGCA TCGCCCCCCA GCAGTGTGCC ATCTGCCTGC AGCCCTTCTG CCACCTATAC
    TGGGGCTGTG CCCGGACTGG CTGCCTCGGC TGCCTGGCCC CGTTCTGCGA GCTCAACCTG
    GGTGACCGGT GTCTGGACGG GGTGCTGAGC AACAACAACT ATGAGTCGGA CGTCCTGAAG
    AATTACCTGG CCACCAGGGG TTTGACTTGG AAAAACATGT TGACTGAGAG CCTCGGGGCT
    CTGCAGAGGG GAGTATTCCT GCTGTCTGAT TACAGAATCA CCGGGAACAC CGTGCTGTGC
    TACTGCTGTG GCCTGCGGAG CTTCCGCGAG CTCACCTATC AGTATCGGCA GAACATTCCT
    GCTTCTGAGC TACCAGCGGC CGTCACGTCC CGTCCTGATT GCTACTGGGG CCGCAACTGC
    CGCACTCAGG TGAAGGCCCA CCACGCAATG AAATTCAATC ACATCTGTGA ACAGACGAGG
    TTCAAGAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005217736.1
    CDS107..2098
    Translation

    Target ORF information:

    RefSeq Version XM_005217679.3
    Organism Bos taurus(cattle)
    Definition Bos taurus checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase (CHFR), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005217679.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGAGCGGC CGGGGGAAGG CGAGCAGCCG CCCCAGCAGC AGCCGTGGGG AAGGCTTCTT 
    CGCCTGGGCG CCGAGGAGGG CGAGCCGCAC GTCCTCCTGA GGAAACGGGA GTGGACCATC
    GGGCGAAGAA GAGGTTGTGA CCTTTCATTC CCTGGCAATA AATTGGTCTC TGGAGATCAC
    TGTAAAATTA CAGTGGATGA AAAATCGGGT CAGGTATCAC TGGAAGATAC CAGTGCCAAT
    GGAACTGTCA TTAACAAGCT GAAGGTTGTT AAGAAGCAAA CTTGCCCTTT ACAGACTGGG
    GATGTCATCT ACTTGGTGTA CAGGAAGAAT GAGCCAGAAC ACAACGTGGC ATACCTCTAC
    GAATCTCTAA ATGAAAAGCA AGGTGTAACA CAAGACTCCT TTGAAGCTAA TAAAGAAAAT
    GTGTTCCACG TGACCAAAGA TACCTCAGGT GCAGGGCGAG GTGACGATCC TCAGGATCCC
    CAGGTCCTGC TGTCGTCGCC CGCCACTCAG GTGTGCTTTG AGGAACCACA GCCATCGACG
    TCCACATCGG ACCTCTTCCC CACGGCCTCT ACCTCTTCCA TGGAGCCCAC CTCCGCAGGC
    CGAGGGCCTC CCTCCAGCTC CAGCTCCAGA GGTACAGACA TCTCTCCAAA GGGGTGTGGT
    CCATCTGTGA CAAGTGATGA AATCTCCAGC TTCCCTTTAA CTCTCCCAGA CAGAGAGGGG
    GCTTCCTTTT CTCTGTCGGA ACCCCAGGAT CAGGAGGATT TGGAGCCTGT TAAGAAGAGA
    GTAAAAGCAG TTGGGGAGCC AGACCTTAAC CTGCCCATAC TGGTCACAGA CCCAGGCAGA
    GACCCTCCCG CTGCCCTTGA CGTCAGAGCC GAGGCCATGA AGCCAGACAA GATGGAGGAG
    ACGCTCACGT GCATCATCTG CCAGGACCTG CTGCACGACT GTGTGAGCCT GCAGCCCTGT
    ATGCACACCT TCTGCGCTGC CTGCTACTCG GGCTGGATGG AGCGGTCTGC CCTGTGCCCC
    ACCTGCCGCT GTCCGGTTGA GCGCATCTGT AAAAACCACA TCCTCAACAA CCTGGTGGAG
    GCGTACCTGC TGCAGCACCC GGACAAGAGG CGCAGCGAGG AGGATCTGCG CAGCATGGCC
    GCCAGGAACA AGATCACACA GGACATGCTG CAGCCCAAGG TCAGGAGGGC CTTCTCCGAC
    GAGGAGGGCA GCTCTGAGGA CCTGCTGGAG CTGTCGGACG TGGACAGTGA GTCCTCGGAC
    GTTAGCCAGC CGTCCATCGT GTGCAGACAG TGCCCTGAGT ACCGCAGGCA GGCGGGGCAG
    CCCCTTCCCT ACCCAGGGCC CGGCAGCGAG CCAGGGGCAC CGCAGGTCCC CGGGGATGCA
    CCGTCCACGT CCGCCAGTGT CACGGCAGCC CAGGACTACG TGTGCGCCCT GCAAGGAAGC
    CACGCCATAT GCACCTGCTG CTTCCAGCCC ATGCCGGACC GGAGAGCGGA GCGTGAGCGG
    GACCCCCGCA TCGCCCCCCA GCAGTGTGCC ATCTGCCTGC AGCCCTTCTG CCACCTATAC
    TGGGGCTGTG CCCGGACTGG CTGCCTCGGC TGCCTGGCCC CGTTCTGCGA GCTCAACCTG
    GGTGACCGGT GTCTGGACGG GGTGCTGAGC AACAACAACT ATGAGTCGGA CGTCCTGAAG
    AATTACCTGG CCACCAGGGG TTTGACTTGG AAAAACATGT TGACTGAGAG CCTCGGGGCT
    CTGCAGAGGG GAGTATTCCT GCTGTCTGAT TACAGAATCA CCGGGAACAC CGTGCTGTGC
    TACTGCTGTG GCCTGCGGAG CTTCCGCGAG CTCACCTATC AGTATCGGCA GAACATTCCT
    GCTTCTGAGC TACCAGCGGC CGTCACGTCC CGTCCTGATT GCTACTGGGG CCGCAACTGC
    CGCACTCAGG TGAAGGCCCA CCACGCAATG AAATTCAATC ACATCTGTGA ACAGACGAGG
    TTCAAGAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa224391
    Clone ID Related Accession (Same CDS sequence) XM_005217679.4 , NM_001205474.3
    Accession Version NM_001205474.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1992bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-12-19
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase CHFR
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000017.1. On Dec 20, 2018 this sequence version replaced NM_001205474.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: GGVH01008198.1, GGUY01128769.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145414 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGAGCGGC CGGGGGAAGG CGAGCAGCCG CCCCAGCAGC AGCCGTGGGG AAGGCTTCTT 
    CGCCTGGGCG CCGAGGAGGG CGAGCCGCAC GTCCTCCTGA GGAAACGGGA GTGGACCATC
    GGGCGAAGAA GAGGTTGTGA CCTTTCATTC CCTGGCAATA AATTGGTCTC TGGAGATCAC
    TGTAAAATTA CAGTGGATGA AAAATCGGGT CAGGTATCAC TGGAAGATAC CAGTGCCAAT
    GGAACTGTCA TTAACAAGCT GAAGGTTGTT AAGAAGCAAA CTTGCCCTTT ACAGACTGGG
    GATGTCATCT ACTTGGTGTA CAGGAAGAAT GAGCCAGAAC ACAACGTGGC ATACCTCTAC
    GAATCTCTAA ATGAAAAGCA AGGTGTAACA CAAGACTCCT TTGAAGCTAA TAAAGAAAAT
    GTGTTCCACG TGACCAAAGA TACCTCAGGT GCAGGGCGAG GTGACGATCC TCAGGATCCC
    CAGGTCCTGC TGTCGTCGCC CGCCACTCAG GTGTGCTTTG AGGAACCACA GCCATCGACG
    TCCACATCGG ACCTCTTCCC CACGGCCTCT ACCTCTTCCA TGGAGCCCAC CTCCGCAGGC
    CGAGGGCCTC CCTCCAGCTC CAGCTCCAGA GGTACAGACA TCTCTCCAAA GGGGTGTGGT
    CCATCTGTGA CAAGTGATGA AATCTCCAGC TTCCCTTTAA CTCTCCCAGA CAGAGAGGGG
    GCTTCCTTTT CTCTGTCGGA ACCCCAGGAT CAGGAGGATT TGGAGCCTGT TAAGAAGAGA
    GTAAAAGCAG TTGGGGAGCC AGACCTTAAC CTGCCCATAC TGGTCACAGA CCCAGGCAGA
    GACCCTCCCG CTGCCCTTGA CGTCAGAGCC GAGGCCATGA AGCCAGACAA GATGGAGGAG
    ACGCTCACGT GCATCATCTG CCAGGACCTG CTGCACGACT GTGTGAGCCT GCAGCCCTGT
    ATGCACACCT TCTGCGCTGC CTGCTACTCG GGCTGGATGG AGCGGTCTGC CCTGTGCCCC
    ACCTGCCGCT GTCCGGTTGA GCGCATCTGT AAAAACCACA TCCTCAACAA CCTGGTGGAG
    GCGTACCTGC TGCAGCACCC GGACAAGAGG CGCAGCGAGG AGGATCTGCG CAGCATGGCC
    GCCAGGAACA AGATCACACA GGACATGCTG CAGCCCAAGG TCAGGAGGGC CTTCTCCGAC
    GAGGAGGGCA GCTCTGAGGA CCTGCTGGAG CTGTCGGACG TGGACAGTGA GTCCTCGGAC
    GTTAGCCAGC CGTCCATCGT GTGCAGACAG TGCCCTGAGT ACCGCAGGCA GGCGGGGCAG
    CCCCTTCCCT ACCCAGGGCC CGGCAGCGAG CCAGGGGCAC CGCAGGTCCC CGGGGATGCA
    CCGTCCACGT CCGCCAGTGT CATGGCAGCC CAGGACTACG TGTGCGCCCT TCAAGGAAGC
    CACGCCATAT GCACCTGCTG CTTCCAGCCC ATGCCGGACC GGAGAGCGGA GCGTGAGCGG
    GACCCCCGCA TCGCCCCCCA GCAGTGTGCC ATCTGCCTGC AGCCCTTCTG CCACCTATAC
    TGGGGCTGTG CCCGGACTGG CTGCCTCGGC TGCCTGGCCC CGTTCTGCGA GCTCAACCTG
    GGTGACCGGT GTCTGGACGG GGTGCTGAGC AACAACAACT ATGAGTCGGA CGTCCTGAAG
    AATTACCTGG CCACCAGGGG TTTGACTTGG AAAAACATGT TGACTGAGAG CCTCGGGGCT
    CTGCAGAGGG GAGTATTCCT GCTGTCTGAT TACAGAATCA CCGGGAACAC CGTGCTGTGC
    TACTGCTGTG GCCTGCGGAG CTTCCGCGAG CTCACCTATC AGTATCGGCA GAACATTCCT
    GCTTCTGAGC TACCAGCGGC CGTCACGTCC CGTCCTGATT GCTACTGGGG CCGCAACTGC
    CGCACTCAGG TGAAGGCCCA CCACGCAATG AAATTCAATC ACATCTGTGA ACAGACGAGG
    TTCAAGAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001192403.2
    CDS1..1992
    Translation

    Target ORF information:

    RefSeq Version NM_001205474.3
    Organism Bos taurus(cattle)
    Definition Bos taurus checkpoint with forkhead and ring finger domains (CHFR), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001205474.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGAGCGGC CGGGGGAAGG CGAGCAGCCG CCCCAGCAGC AGCCGTGGGG AAGGCTTCTT 
    CGCCTGGGCG CCGAGGAGGG CGAGCCGCAC GTCCTCCTGA GGAAACGGGA GTGGACCATC
    GGGCGAAGAA GAGGTTGTGA CCTTTCATTC CCTGGCAATA AATTGGTCTC TGGAGATCAC
    TGTAAAATTA CAGTGGATGA AAAATCGGGT CAGGTATCAC TGGAAGATAC CAGTGCCAAT
    GGAACTGTCA TTAACAAGCT GAAGGTTGTT AAGAAGCAAA CTTGCCCTTT ACAGACTGGG
    GATGTCATCT ACTTGGTGTA CAGGAAGAAT GAGCCAGAAC ACAACGTGGC ATACCTCTAC
    GAATCTCTAA ATGAAAAGCA AGGTGTAACA CAAGACTCCT TTGAAGCTAA TAAAGAAAAT
    GTGTTCCACG TGACCAAAGA TACCTCAGGT GCAGGGCGAG GTGACGATCC TCAGGATCCC
    CAGGTCCTGC TGTCGTCGCC CGCCACTCAG GTGTGCTTTG AGGAACCACA GCCATCGACG
    TCCACATCGG ACCTCTTCCC CACGGCCTCT ACCTCTTCCA TGGAGCCCAC CTCCGCAGGC
    CGAGGGCCTC CCTCCAGCTC CAGCTCCAGA GGTACAGACA TCTCTCCAAA GGGGTGTGGT
    CCATCTGTGA CAAGTGATGA AATCTCCAGC TTCCCTTTAA CTCTCCCAGA CAGAGAGGGG
    GCTTCCTTTT CTCTGTCGGA ACCCCAGGAT CAGGAGGATT TGGAGCCTGT TAAGAAGAGA
    GTAAAAGCAG TTGGGGAGCC AGACCTTAAC CTGCCCATAC TGGTCACAGA CCCAGGCAGA
    GACCCTCCCG CTGCCCTTGA CGTCAGAGCC GAGGCCATGA AGCCAGACAA GATGGAGGAG
    ACGCTCACGT GCATCATCTG CCAGGACCTG CTGCACGACT GTGTGAGCCT GCAGCCCTGT
    ATGCACACCT TCTGCGCTGC CTGCTACTCG GGCTGGATGG AGCGGTCTGC CCTGTGCCCC
    ACCTGCCGCT GTCCGGTTGA GCGCATCTGT AAAAACCACA TCCTCAACAA CCTGGTGGAG
    GCGTACCTGC TGCAGCACCC GGACAAGAGG CGCAGCGAGG AGGATCTGCG CAGCATGGCC
    GCCAGGAACA AGATCACACA GGACATGCTG CAGCCCAAGG TCAGGAGGGC CTTCTCCGAC
    GAGGAGGGCA GCTCTGAGGA CCTGCTGGAG CTGTCGGACG TGGACAGTGA GTCCTCGGAC
    GTTAGCCAGC CGTCCATCGT GTGCAGACAG TGCCCTGAGT ACCGCAGGCA GGCGGGGCAG
    CCCCTTCCCT ACCCAGGGCC CGGCAGCGAG CCAGGGGCAC CGCAGGTCCC CGGGGATGCA
    CCGTCCACGT CCGCCAGTGT CATGGCAGCC CAGGACTACG TGTGCGCCCT TCAAGGAAGC
    CACGCCATAT GCACCTGCTG CTTCCAGCCC ATGCCGGACC GGAGAGCGGA GCGTGAGCGG
    GACCCCCGCA TCGCCCCCCA GCAGTGTGCC ATCTGCCTGC AGCCCTTCTG CCACCTATAC
    TGGGGCTGTG CCCGGACTGG CTGCCTCGGC TGCCTGGCCC CGTTCTGCGA GCTCAACCTG
    GGTGACCGGT GTCTGGACGG GGTGCTGAGC AACAACAACT ATGAGTCGGA CGTCCTGAAG
    AATTACCTGG CCACCAGGGG TTTGACTTGG AAAAACATGT TGACTGAGAG CCTCGGGGCT
    CTGCAGAGGG GAGTATTCCT GCTGTCTGAT TACAGAATCA CCGGGAACAC CGTGCTGTGC
    TACTGCTGTG GCCTGCGGAG CTTCCGCGAG CTCACCTATC AGTATCGGCA GAACATTCCT
    GCTTCTGAGC TACCAGCGGC CGTCACGTCC CGTCCTGATT GCTACTGGGG CCGCAACTGC
    CGCACTCAGG TGAAGGCCCA CCACGCAATG AAATTCAATC ACATCTGTGA ACAGACGAGG
    TTCAAGAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa224391
    Clone ID Related Accession (Same CDS sequence) XM_005217679.4 , NM_001205474.3
    Accession Version XM_005217679.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1992bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase CHFR isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005217679.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGAGCGGC CGGGGGAAGG CGAGCAGCCG CCCCAGCAGC AGCCGTGGGG AAGGCTTCTT 
    CGCCTGGGCG CCGAGGAGGG CGAGCCGCAC GTCCTCCTGA GGAAACGGGA GTGGACCATC
    GGGCGAAGAA GAGGTTGTGA CCTTTCATTC CCTGGCAATA AATTGGTCTC TGGAGATCAC
    TGTAAAATTA CAGTGGATGA AAAATCGGGT CAGGTATCAC TGGAAGATAC CAGTGCCAAT
    GGAACTGTCA TTAACAAGCT GAAGGTTGTT AAGAAGCAAA CTTGCCCTTT ACAGACTGGG
    GATGTCATCT ACTTGGTGTA CAGGAAGAAT GAGCCAGAAC ACAACGTGGC ATACCTCTAC
    GAATCTCTAA ATGAAAAGCA AGGTGTAACA CAAGACTCCT TTGAAGCTAA TAAAGAAAAT
    GTGTTCCACG TGACCAAAGA TACCTCAGGT GCAGGGCGAG GTGACGATCC TCAGGATCCC
    CAGGTCCTGC TGTCGTCGCC CGCCACTCAG GTGTGCTTTG AGGAACCACA GCCATCGACG
    TCCACATCGG ACCTCTTCCC CACGGCCTCT ACCTCTTCCA TGGAGCCCAC CTCCGCAGGC
    CGAGGGCCTC CCTCCAGCTC CAGCTCCAGA GGTACAGACA TCTCTCCAAA GGGGTGTGGT
    CCATCTGTGA CAAGTGATGA AATCTCCAGC TTCCCTTTAA CTCTCCCAGA CAGAGAGGGG
    GCTTCCTTTT CTCTGTCGGA ACCCCAGGAT CAGGAGGATT TGGAGCCTGT TAAGAAGAGA
    GTAAAAGCAG TTGGGGAGCC AGACCTTAAC CTGCCCATAC TGGTCACAGA CCCAGGCAGA
    GACCCTCCCG CTGCCCTTGA CGTCAGAGCC GAGGCCATGA AGCCAGACAA GATGGAGGAG
    ACGCTCACGT GCATCATCTG CCAGGACCTG CTGCACGACT GTGTGAGCCT GCAGCCCTGT
    ATGCACACCT TCTGCGCTGC CTGCTACTCG GGCTGGATGG AGCGGTCTGC CCTGTGCCCC
    ACCTGCCGCT GTCCGGTTGA GCGCATCTGT AAAAACCACA TCCTCAACAA CCTGGTGGAG
    GCGTACCTGC TGCAGCACCC GGACAAGAGG CGCAGCGAGG AGGATCTGCG CAGCATGGCC
    GCCAGGAACA AGATCACACA GGACATGCTG CAGCCCAAGG TCAGGAGGGC CTTCTCCGAC
    GAGGAGGGCA GCTCTGAGGA CCTGCTGGAG CTGTCGGACG TGGACAGTGA GTCCTCGGAC
    GTTAGCCAGC CGTCCATCGT GTGCAGACAG TGCCCTGAGT ACCGCAGGCA GGCGGGGCAG
    CCCCTTCCCT ACCCAGGGCC CGGCAGCGAG CCAGGGGCAC CGCAGGTCCC CGGGGATGCA
    CCGTCCACGT CCGCCAGTGT CATGGCAGCC CAGGACTACG TGTGCGCCCT TCAAGGAAGC
    CACGCCATAT GCACCTGCTG CTTCCAGCCC ATGCCGGACC GGAGAGCGGA GCGTGAGCGG
    GACCCCCGCA TCGCCCCCCA GCAGTGTGCC ATCTGCCTGC AGCCCTTCTG CCACCTATAC
    TGGGGCTGTG CCCGGACTGG CTGCCTCGGC TGCCTGGCCC CGTTCTGCGA GCTCAACCTG
    GGTGACCGGT GTCTGGACGG GGTGCTGAGC AACAACAACT ATGAGTCGGA CGTCCTGAAG
    AATTACCTGG CCACCAGGGG TTTGACTTGG AAAAACATGT TGACTGAGAG CCTCGGGGCT
    CTGCAGAGGG GAGTATTCCT GCTGTCTGAT TACAGAATCA CCGGGAACAC CGTGCTGTGC
    TACTGCTGTG GCCTGCGGAG CTTCCGCGAG CTCACCTATC AGTATCGGCA GAACATTCCT
    GCTTCTGAGC TACCAGCGGC CGTCACGTCC CGTCCTGATT GCTACTGGGG CCGCAACTGC
    CGCACTCAGG TGAAGGCCCA CCACGCAATG AAATTCAATC ACATCTGTGA ACAGACGAGG
    TTCAAGAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005217736.2
    CDS154..2145
    Translation

    Target ORF information:

    RefSeq Version XM_005217679.4
    Organism Bos taurus(cattle)
    Definition Bos taurus checkpoint with forkhead and ring finger domains (CHFR), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005217679.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGAGCGGC CGGGGGAAGG CGAGCAGCCG CCCCAGCAGC AGCCGTGGGG AAGGCTTCTT 
    CGCCTGGGCG CCGAGGAGGG CGAGCCGCAC GTCCTCCTGA GGAAACGGGA GTGGACCATC
    GGGCGAAGAA GAGGTTGTGA CCTTTCATTC CCTGGCAATA AATTGGTCTC TGGAGATCAC
    TGTAAAATTA CAGTGGATGA AAAATCGGGT CAGGTATCAC TGGAAGATAC CAGTGCCAAT
    GGAACTGTCA TTAACAAGCT GAAGGTTGTT AAGAAGCAAA CTTGCCCTTT ACAGACTGGG
    GATGTCATCT ACTTGGTGTA CAGGAAGAAT GAGCCAGAAC ACAACGTGGC ATACCTCTAC
    GAATCTCTAA ATGAAAAGCA AGGTGTAACA CAAGACTCCT TTGAAGCTAA TAAAGAAAAT
    GTGTTCCACG TGACCAAAGA TACCTCAGGT GCAGGGCGAG GTGACGATCC TCAGGATCCC
    CAGGTCCTGC TGTCGTCGCC CGCCACTCAG GTGTGCTTTG AGGAACCACA GCCATCGACG
    TCCACATCGG ACCTCTTCCC CACGGCCTCT ACCTCTTCCA TGGAGCCCAC CTCCGCAGGC
    CGAGGGCCTC CCTCCAGCTC CAGCTCCAGA GGTACAGACA TCTCTCCAAA GGGGTGTGGT
    CCATCTGTGA CAAGTGATGA AATCTCCAGC TTCCCTTTAA CTCTCCCAGA CAGAGAGGGG
    GCTTCCTTTT CTCTGTCGGA ACCCCAGGAT CAGGAGGATT TGGAGCCTGT TAAGAAGAGA
    GTAAAAGCAG TTGGGGAGCC AGACCTTAAC CTGCCCATAC TGGTCACAGA CCCAGGCAGA
    GACCCTCCCG CTGCCCTTGA CGTCAGAGCC GAGGCCATGA AGCCAGACAA GATGGAGGAG
    ACGCTCACGT GCATCATCTG CCAGGACCTG CTGCACGACT GTGTGAGCCT GCAGCCCTGT
    ATGCACACCT TCTGCGCTGC CTGCTACTCG GGCTGGATGG AGCGGTCTGC CCTGTGCCCC
    ACCTGCCGCT GTCCGGTTGA GCGCATCTGT AAAAACCACA TCCTCAACAA CCTGGTGGAG
    GCGTACCTGC TGCAGCACCC GGACAAGAGG CGCAGCGAGG AGGATCTGCG CAGCATGGCC
    GCCAGGAACA AGATCACACA GGACATGCTG CAGCCCAAGG TCAGGAGGGC CTTCTCCGAC
    GAGGAGGGCA GCTCTGAGGA CCTGCTGGAG CTGTCGGACG TGGACAGTGA GTCCTCGGAC
    GTTAGCCAGC CGTCCATCGT GTGCAGACAG TGCCCTGAGT ACCGCAGGCA GGCGGGGCAG
    CCCCTTCCCT ACCCAGGGCC CGGCAGCGAG CCAGGGGCAC CGCAGGTCCC CGGGGATGCA
    CCGTCCACGT CCGCCAGTGT CATGGCAGCC CAGGACTACG TGTGCGCCCT TCAAGGAAGC
    CACGCCATAT GCACCTGCTG CTTCCAGCCC ATGCCGGACC GGAGAGCGGA GCGTGAGCGG
    GACCCCCGCA TCGCCCCCCA GCAGTGTGCC ATCTGCCTGC AGCCCTTCTG CCACCTATAC
    TGGGGCTGTG CCCGGACTGG CTGCCTCGGC TGCCTGGCCC CGTTCTGCGA GCTCAACCTG
    GGTGACCGGT GTCTGGACGG GGTGCTGAGC AACAACAACT ATGAGTCGGA CGTCCTGAAG
    AATTACCTGG CCACCAGGGG TTTGACTTGG AAAAACATGT TGACTGAGAG CCTCGGGGCT
    CTGCAGAGGG GAGTATTCCT GCTGTCTGAT TACAGAATCA CCGGGAACAC CGTGCTGTGC
    TACTGCTGTG GCCTGCGGAG CTTCCGCGAG CTCACCTATC AGTATCGGCA GAACATTCCT
    GCTTCTGAGC TACCAGCGGC CGTCACGTCC CGTCCTGATT GCTACTGGGG CCGCAACTGC
    CGCACTCAGG TGAAGGCCCA CCACGCAATG AAATTCAATC ACATCTGTGA ACAGACGAGG
    TTCAAGAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa224392
    Clone ID Related Accession (Same CDS sequence) XM_024977446.1
    Accession Version XM_024977446.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1884bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase CHFR isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGAGCGGC CGGGGGAAGG CGAGCAGCCG CCCCAGCAGC AGCCGTGGGG AAGGCTTCTT 
    CGCCTGGGCG CCGAGGAGGG CGAGCCGCAC GTCCTCCTGA GGAAACGGGA GTGGACCATC
    GGGCGAAGAA GAGGTTGTGA CCTTTCATTC CCTGGCAATA AATTGGTCTC TGGAGATCAC
    TGTAAAATTA CAGTGGATGA AAAATCGGGT CAGGTATCAC TGGAAGATAC CAGTGCCAAT
    GGAACTGTCA TTAACAAGCT GAAGGTTGTT AAGAAGCAAA CTTGCCCTTT ACAGACTGGG
    GATGTCATCT ACTTGGTGTA CAGGAAGAAT GAGCCAGAAC ACAACGTGGC ATACCTCTAC
    GAATCTCTAA ATGAAAAGCA AGGTGTAACA CAAGACTCCT TTGAAGCTAA TAAAGAAAAT
    GTGTTCCACG TGACCAAAGA TACCTCAGGT GCAGGGCGAG GTGACGATCC TCAGGATCCC
    CAGGTCCTGC TGTCGTCGCC CGCCACTCAG GTGTGCTTTG AGGAACCACA GCCATCGACG
    TCCACATCGG ACCTCTTCCC CACGGCCTCT ACCTCTTCCA TGGAGCCCAC CTCCGCAGGC
    CGAGGGCCTC CCTCCAGCTC CAGCTCCAGA GGTACAGACA TCTCTCCAAA GGGGTGTGGT
    CCATCTGTGA CAAGTGATGA AATCTCCAGC TTCCCTTTAA CTCTCCCAGA CAGAGAGGGG
    GCTTCCTTTT CTCTGTCGGA ACCCCAGGAT CAGGAGGATT TGGAGCCTGT TAAGAAGAGA
    GTAAAAGCAG TTGGGGAGCC AGACCTTAAC CTGCCCATAC TGGTCACAGA CCCAGGCAGA
    GACCCTCCCG CTGCCCTTGA CGTCAGAGCC GAGGCCATGA AGCCAGACAA GATGGAGGAG
    ACGCTCACGT GCATCATCTG CCAGGACCTG CTGCACGACT GTGTGAGCCT GCAGCCCTGT
    ATGCACACCT TCTGCGCTGC CTGCTACTCG GGCTGGATGG AGCGGTCTGC CCTGTGCCCC
    ACCTGCCGCT GTCCGGTTGA GCGCATCTGT AAAAACCACA TCCTCAACAA CCTGGTGGAG
    GCGTACCTGC TGCAGCACCC GGACAAGAGG CGCAGCGAGG AGGATCTGCG CAGCATGGCC
    GCCAGGAACA AGATCACACA GGACATGCTG CAGCCCAAGG TCAGGAGGGC CTTCTCCGAC
    GAGGAGGGCA GCTCTGAGGA CCTGCTGGAG CTGTCGGACG TGGACAGTGA GTCCTCGGAC
    GTTAGCCAGC CGTCCATCGT GTGCAGACAG TGCCCTGAGT ACCGCAGGCA GGCGGGGCAG
    CCCCTTCCCT ACCCAGGGCC CGGCAGCGAG CCAGGGGCAC CGCAGGTCCC CGGGGATGCA
    CCGTCCACGT CCGCCAGTGT CATGGCAGCC CAGGACTACG TGTGCGCCCT TCAAGGAAGC
    CACGCCATAT GCACCTGCTG CTTCCAGCCC ATGCCGGACC GGAGAGCGGA GCGTGAGCGG
    GACCCCCGCA TCGCCCCCCA GCAGTGTGCC ATCTGCCTGC AGCCCTTCTG CCACCTATAC
    TGGGGCTGTG CCCGGACTGG CTGCCTCGGC TGCCTGGCCC CGTTCTGCGA GCTCAACCTG
    GGTGACCGGT GTCTGGACGG GGTGCTGAGC AACAACAACT ATGAGTCGGA CGTCCTGAAG
    AATTACCTGG CCACCAGGGG TTTGACTTGG AAAAACATGT TGACTGAGAG CCTCGGGGCT
    CTGCAGAGGG GAGTATTCCT GCTGTCTGCG GCCGTCACGT CCCGTCCTGA TTGCTACTGG
    GGCCGCAACT GCCGCACTCA GGTGAAGGCC CACCACGCAA TGAAATTCAA TCACATCTGT
    GAACAGACGA GGTTCAAGAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024833214.1
    CDS144..2027
    Translation

    Target ORF information:

    RefSeq Version XM_024977446.1
    Organism Bos taurus(cattle)
    Definition Bos taurus checkpoint with forkhead and ring finger domains (CHFR), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024977446.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGAGCGGC CGGGGGAAGG CGAGCAGCCG CCCCAGCAGC AGCCGTGGGG AAGGCTTCTT 
    CGCCTGGGCG CCGAGGAGGG CGAGCCGCAC GTCCTCCTGA GGAAACGGGA GTGGACCATC
    GGGCGAAGAA GAGGTTGTGA CCTTTCATTC CCTGGCAATA AATTGGTCTC TGGAGATCAC
    TGTAAAATTA CAGTGGATGA AAAATCGGGT CAGGTATCAC TGGAAGATAC CAGTGCCAAT
    GGAACTGTCA TTAACAAGCT GAAGGTTGTT AAGAAGCAAA CTTGCCCTTT ACAGACTGGG
    GATGTCATCT ACTTGGTGTA CAGGAAGAAT GAGCCAGAAC ACAACGTGGC ATACCTCTAC
    GAATCTCTAA ATGAAAAGCA AGGTGTAACA CAAGACTCCT TTGAAGCTAA TAAAGAAAAT
    GTGTTCCACG TGACCAAAGA TACCTCAGGT GCAGGGCGAG GTGACGATCC TCAGGATCCC
    CAGGTCCTGC TGTCGTCGCC CGCCACTCAG GTGTGCTTTG AGGAACCACA GCCATCGACG
    TCCACATCGG ACCTCTTCCC CACGGCCTCT ACCTCTTCCA TGGAGCCCAC CTCCGCAGGC
    CGAGGGCCTC CCTCCAGCTC CAGCTCCAGA GGTACAGACA TCTCTCCAAA GGGGTGTGGT
    CCATCTGTGA CAAGTGATGA AATCTCCAGC TTCCCTTTAA CTCTCCCAGA CAGAGAGGGG
    GCTTCCTTTT CTCTGTCGGA ACCCCAGGAT CAGGAGGATT TGGAGCCTGT TAAGAAGAGA
    GTAAAAGCAG TTGGGGAGCC AGACCTTAAC CTGCCCATAC TGGTCACAGA CCCAGGCAGA
    GACCCTCCCG CTGCCCTTGA CGTCAGAGCC GAGGCCATGA AGCCAGACAA GATGGAGGAG
    ACGCTCACGT GCATCATCTG CCAGGACCTG CTGCACGACT GTGTGAGCCT GCAGCCCTGT
    ATGCACACCT TCTGCGCTGC CTGCTACTCG GGCTGGATGG AGCGGTCTGC CCTGTGCCCC
    ACCTGCCGCT GTCCGGTTGA GCGCATCTGT AAAAACCACA TCCTCAACAA CCTGGTGGAG
    GCGTACCTGC TGCAGCACCC GGACAAGAGG CGCAGCGAGG AGGATCTGCG CAGCATGGCC
    GCCAGGAACA AGATCACACA GGACATGCTG CAGCCCAAGG TCAGGAGGGC CTTCTCCGAC
    GAGGAGGGCA GCTCTGAGGA CCTGCTGGAG CTGTCGGACG TGGACAGTGA GTCCTCGGAC
    GTTAGCCAGC CGTCCATCGT GTGCAGACAG TGCCCTGAGT ACCGCAGGCA GGCGGGGCAG
    CCCCTTCCCT ACCCAGGGCC CGGCAGCGAG CCAGGGGCAC CGCAGGTCCC CGGGGATGCA
    CCGTCCACGT CCGCCAGTGT CATGGCAGCC CAGGACTACG TGTGCGCCCT TCAAGGAAGC
    CACGCCATAT GCACCTGCTG CTTCCAGCCC ATGCCGGACC GGAGAGCGGA GCGTGAGCGG
    GACCCCCGCA TCGCCCCCCA GCAGTGTGCC ATCTGCCTGC AGCCCTTCTG CCACCTATAC
    TGGGGCTGTG CCCGGACTGG CTGCCTCGGC TGCCTGGCCC CGTTCTGCGA GCTCAACCTG
    GGTGACCGGT GTCTGGACGG GGTGCTGAGC AACAACAACT ATGAGTCGGA CGTCCTGAAG
    AATTACCTGG CCACCAGGGG TTTGACTTGG AAAAACATGT TGACTGAGAG CCTCGGGGCT
    CTGCAGAGGG GAGTATTCCT GCTGTCTGCG GCCGTCACGT CCCGTCCTGA TTGCTACTGG
    GGCCGCAACT GCCGCACTCA GGTGAAGGCC CACCACGCAA TGAAATTCAA TCACATCTGT
    GAACAGACGA GGTTCAAGAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.