CHFR cDNA ORF clone, Canis lupus familiaris(dog)

The following CHFR gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CHFR cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe26048 XM_014107998.1
Latest version!
Canis lupus familiaris checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase (CHFR), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OCe26048 XM_534631.5
Latest version!
Canis lupus familiaris checkpoint with forkhead and ring finger domains (CHFR), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OCe26048 XM_022410413.1
Latest version!
Canis lupus familiaris checkpoint with forkhead and ring finger domains (CHFR), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OCe26048 XM_005636063.2
Latest version!
Canis lupus familiaris checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase (CHFR), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OCe26048 XM_022410412.1
Latest version!
Canis lupus familiaris checkpoint with forkhead and ring finger domains (CHFR), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OCe26049 XM_022410416.1
Latest version!
Canis lupus familiaris checkpoint with forkhead and ring finger domains (CHFR), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OCe26049 XM_005636065.1
Latest version!
Canis lupus familiaris checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase (CHFR), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OCe282435 XM_022410415.1
Latest version!
Canis lupus familiaris checkpoint with forkhead and ring finger domains (CHFR), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OCe282435 XM_022410414.1
Latest version!
Canis lupus familiaris checkpoint with forkhead and ring finger domains (CHFR), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
View Old Accession Versions >>
OCe26048 XM_534631.4 Canis lupus familiaris checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase (CHFR), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe26048
    Clone ID Related Accession (Same CDS sequence) XM_534631.5 , XM_534631.4 , XM_022410413.1 , XM_014107998.1 , XM_005636063.2 , XM_022410412.1
    Accession Version XM_534631.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1986bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product E3 ubiquitin-protein ligase CHFR isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006608.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_534631.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGAGCAGC GCCGGGAAGG CGAGCAGCCG CCGCAGCAGC AGCCGTGGGG GAGGCTCCTC 
    CGCTTGGGCG CCGGGGACGG CGAGCCGCAC GTCCTCCTGA GGAACCGGGA GTGGACCATC
    GGGCGGAGGA GAGGTTGTGA CCTTTCATTC CCTGGCAATA AACTGGTCTC TGGAGATCAC
    TGTAAAATTA TAGTGGATGA AAAATCTGGT CAGGTGTCAC TAGAAGATAC CAGCACCAAT
    GGAACAGTAA TTAACAAGCT GAAGGTTGTT AAGAAGCAGA CCTGCCCTTT GCAGACGGGG
    GATGTCATCT ACTTGGTGTA CAGGAAGAAT GAGCCAGAAC ACAATGTGGC ATACCTCTAT
    GAATCTTTAA ATGAGAAGCA GGACATCACA CAAGACTCCT TTGAAGCCAA TAAAGAGAAT
    GTGTTCCGTG TGACCAAAGA TACCTCAGGT GCAGGGCAAG GTGACGACCC CCAGGTCCTG
    CCGTCATCGC CCACCACTCA GGCGTGCTTT GAGGAACCAC AGCCATCAAC ATCCACATCA
    GACCTCTTCC CCACGGCTTC TACCTCTTCC ATGGAGCCCA CCTCTGCAGG GCAAGCACCT
    TCCTCCAGTT CTGCTTCCAG AGATGCATAC ATCTCCCCAA AAGAGTGTGG TCCATCTGTG
    GCAAGTGATG AAATCCCCAT CTTTCCTTCA GCTCTGCCAG ACAGAGAGAG AGCATCCTTT
    TCTCTGTCGG AACCCCAGGA TCAGGAGGAT TTGGAGCCCA TCAGGAAGAA AATGAAAGGA
    GGTGGGGACC CTGACCTTAC CCTGCAGTTC TTGGTTGCAG ACCAGTGTAG AGACACCCAC
    ACCACCCTTG GGAATGTTAG ATCCGAGGCT GTGAAGCCTG ACAAGATGGA GGAGACACTC
    ACATGCATCA TCTGCCAGGA CCTGCTGCAT GATTGTGTGA GCCTGCAGCC CTGCATGCAC
    ACCTTCTGTG CAGCCTGCTA CTCAGGCTGG ATGGAGCGTT CTACCTGGTG TCCCACCTGC
    CGCTGTCCAG TCGAGAGGAT CTGTAAAAAC CACATCCTGA ACAACCTGGT GGAGGCCTAC
    CTTCTCCAGC ACCCAGACAA GAGTCGCAGT GAGGAAGATG TGCGGAGCAT GGCTGCCAGG
    AACAAGATCA CTCAAGACAT GTTGCAACCC AAGGTCAGAA GGTCTTTCTC TGATGAGGAG
    GGCAGTTCGG AAGATCTGCT AGAGCTGTCA GATGTGGACA GTGAATCATC GGATGTTAGC
    CAGCCGTACA TTGTATGCAG GCAGTGCCCT GAGTACCGGA GGCAGGGTGG GCAGGCCCTC
    CCCTGCCCAG GGCCTAACAG TGAGCCAGGG GTACCACAGG CTGCTGGGGA TGCGCCGTCA
    ACCTCCACCA GTGTCAAGAC AGCCCAGGAT TATGTGTGCG CCCTGCAAGG AAGCCATGCC
    ATATGCACCT GCTGCTTCCA GCCCATGCCT GACCGGAGGG CAGAACGTGA GCAGGACCCC
    CGCATCGCCC CCCAGCAGTG TGCCGTTTGC CTACAGCCTT TCTGCCACCT GTACTGGGGC
    TGCGCCCGGG CGGGCTGCCT TGGCTGCCTG GCCCCATTCT GTGAGCTAGA CCTGGGCGAC
    AGGTGTCTGG ACGGGGTTCT GAGCAACAAC CACTACGAGT CAGACGTCCT GAAGAATTAT
    CTGGCAACCA GGGGTTTGAC ATGGAAAAAT ATGTTGACAG AAAGTCTGGT GGCTTTGCAG
    AGGGGAGCGT TTTTACTATC TGATTACAGA ATCACGGGGA ACACCGTGCT GTGTTACTGC
    TGTGGCCTGC GGAGCTTCCG AGAGCTGACC TACCAGTATC GGCAGAACAT CCCTGCTTCT
    GAGTTGCCAG TGGCTGTAAC ATCCCGTCCT GATTGCTATT GGGGGCGCAA CTGCCGCACT
    CAGGTGAAGG CCCACCACGC AATGAAATTC AATCATATCT GTGAACAGAC AAGGTTCAAG
    AACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_534631.3
    CDS166..2151
    Translation

    Target ORF information:

    RefSeq Version XM_534631.5
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris checkpoint with forkhead and ring finger domains (CHFR), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_534631.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGAGCAGC GCCGGGAAGG CGAGCAGCCG CCGCAGCAGC AGCCGTGGGG GAGGCTCCTC 
    CGCTTGGGCG CCGGGGACGG CGAGCCGCAC GTCCTCCTGA GGAACCGGGA GTGGACCATC
    GGGCGGAGGA GAGGTTGTGA CCTTTCATTC CCTGGCAATA AACTGGTCTC TGGAGATCAC
    TGTAAAATTA TAGTGGATGA AAAATCTGGT CAGGTGTCAC TAGAAGATAC CAGCACCAAT
    GGAACAGTAA TTAACAAGCT GAAGGTTGTT AAGAAGCAGA CCTGCCCTTT GCAGACGGGG
    GATGTCATCT ACTTGGTGTA CAGGAAGAAT GAGCCAGAAC ACAATGTGGC ATACCTCTAT
    GAATCTTTAA ATGAGAAGCA GGACATCACA CAAGACTCCT TTGAAGCCAA TAAAGAGAAT
    GTGTTCCGTG TGACCAAAGA TACCTCAGGT GCAGGGCAAG GTGACGACCC CCAGGTCCTG
    CCGTCATCGC CCACCACTCA GGCGTGCTTT GAGGAACCAC AGCCATCAAC ATCCACATCA
    GACCTCTTCC CCACGGCTTC TACCTCTTCC ATGGAGCCCA CCTCTGCAGG GCAAGCACCT
    TCCTCCAGTT CTGCTTCCAG AGATGCATAC ATCTCCCCAA AAGAGTGTGG TCCATCTGTG
    GCAAGTGATG AAATCCCCAT CTTTCCTTCA GCTCTGCCAG ACAGAGAGAG AGCATCCTTT
    TCTCTGTCGG AACCCCAGGA TCAGGAGGAT TTGGAGCCCA TCAGGAAGAA AATGAAAGGA
    GGTGGGGACC CTGACCTTAC CCTGCAGTTC TTGGTTGCAG ACCAGTGTAG AGACACCCAC
    ACCACCCTTG GGAATGTTAG ATCCGAGGCT GTGAAGCCTG ACAAGATGGA GGAGACACTC
    ACATGCATCA TCTGCCAGGA CCTGCTGCAT GATTGTGTGA GCCTGCAGCC CTGCATGCAC
    ACCTTCTGTG CAGCCTGCTA CTCAGGCTGG ATGGAGCGTT CTACCTGGTG TCCCACCTGC
    CGCTGTCCAG TCGAGAGGAT CTGTAAAAAC CACATCCTGA ACAACCTGGT GGAGGCCTAC
    CTTCTCCAGC ACCCAGACAA GAGTCGCAGT GAGGAAGATG TGCGGAGCAT GGCTGCCAGG
    AACAAGATCA CTCAAGACAT GTTGCAACCC AAGGTCAGAA GGTCTTTCTC TGATGAGGAG
    GGCAGTTCGG AAGATCTGCT AGAGCTGTCA GATGTGGACA GTGAATCATC GGATGTTAGC
    CAGCCGTACA TTGTATGCAG GCAGTGCCCT GAGTACCGGA GGCAGGGTGG GCAGGCCCTC
    CCCTGCCCAG GGCCTAACAG TGAGCCAGGG GTACCACAGG CTGCTGGGGA TGCGCCGTCA
    ACCTCCACCA GTGTCAAGAC AGCCCAGGAT TATGTGTGCG CCCTGCAAGG AAGCCATGCC
    ATATGCACCT GCTGCTTCCA GCCCATGCCT GACCGGAGGG CAGAACGTGA GCAGGACCCC
    CGCATCGCCC CCCAGCAGTG TGCCGTTTGC CTACAGCCTT TCTGCCACCT GTACTGGGGC
    TGCGCCCGGG CGGGCTGCCT TGGCTGCCTG GCCCCATTCT GTGAGCTAGA CCTGGGCGAC
    AGGTGTCTGG ACGGGGTTCT GAGCAACAAC CACTACGAGT CAGACGTCCT GAAGAATTAT
    CTGGCAACCA GGGGTTTGAC ATGGAAAAAT ATGTTGACAG AAAGTCTGGT GGCTTTGCAG
    AGGGGAGCGT TTTTACTATC TGATTACAGA ATCACGGGGA ACACCGTGCT GTGTTACTGC
    TGTGGCCTGC GGAGCTTCCG AGAGCTGACC TACCAGTATC GGCAGAACAT CCCTGCTTCT
    GAGTTGCCAG TGGCTGTAAC ATCCCGTCCT GATTGCTATT GGGGGCGCAA CTGCCGCACT
    CAGGTGAAGG CCCACCACGC AATGAAATTC AATCATATCT GTGAACAGAC AAGGTTCAAG
    AACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe26048
    Clone ID Related Accession (Same CDS sequence) XM_534631.5 , XM_534631.4 , XM_022410413.1 , XM_014107998.1 , XM_005636063.2 , XM_022410412.1
    Accession Version XM_534631.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1986bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product E3 ubiquitin-protein ligase CHFR isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726106.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 24, 2013 this sequence version replaced XM_534631.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGAGCAGC GCCGGGAAGG CGAGCAGCCG CCGCAGCAGC AGCCGTGGGG GAGGCTCCTC 
    CGCTTGGGCG CCGGGGACGG CGAGCCGCAC GTCCTCCTGA GGAACCGGGA GTGGACCATC
    GGGCGGAGGA GAGGTTGTGA CCTTTCATTC CCTGGCAATA AACTGGTCTC TGGAGATCAC
    TGTAAAATTA TAGTGGATGA AAAATCTGGT CAGGTGTCAC TAGAAGATAC CAGCACCAAT
    GGAACAGTAA TTAACAAGCT GAAGGTTGTT AAGAAGCAGA CCTGCCCTTT GCAGACGGGG
    GATGTCATCT ACTTGGTGTA CAGGAAGAAT GAGCCAGAAC ACAATGTGGC ATACCTCTAT
    GAATCTTTAA ATGAGAAGCA GGACATCACA CAAGACTCCT TTGAAGCCAA TAAAGAGAAT
    GTGTTCCGTG TGACCAAAGA TACCTCAGGT GCAGGGCAAG GTGACGACCC CCAGGTCCTG
    CCGTCATCGC CCACCACTCA GGCGTGCTTT GAGGAACCAC AGCCATCAAC ATCCACATCA
    GACCTCTTCC CCACGGCTTC TACCTCTTCC ATGGAGCCCA CCTCTGCAGG GCAAGCACCT
    TCCTCCAGTT CTGCTTCCAG AGATGCATAC ATCTCCCCAA AAGAGTGTGG TCCATCTGTG
    GCAAGTGATG AAATCCCCAT CTTTCCTTCA GCTCTGCCAG ACAGAGAGAG AGCATCCTTT
    TCTCTGTCGG AACCCCAGGA TCAGGAGGAT TTGGAGCCCA TCAGGAAGAA AATGAAAGGA
    GGTGGGGACC CTGACCTTAC CCTGCAGTTC TTGGTTGCAG ACCAGTGTAG AGACACCCAC
    ACCACCCTTG GGAATGTTAG ATCCGAGGCT GTGAAGCCTG ACAAGATGGA GGAGACACTC
    ACATGCATCA TCTGCCAGGA CCTGCTGCAT GATTGTGTGA GCCTGCAGCC CTGCATGCAC
    ACCTTCTGTG CAGCCTGCTA CTCAGGCTGG ATGGAGCGTT CTACCTGGTG TCCCACCTGC
    CGCTGTCCAG TCGAGAGGAT CTGTAAAAAC CACATCCTGA ACAACCTGGT GGAGGCCTAC
    CTTCTCCAGC ACCCAGACAA GAGTCGCAGT GAGGAAGATG TGCGGAGCAT GGCTGCCAGG
    AACAAGATCA CTCAAGACAT GTTGCAACCC AAGGTCAGAA GGTCTTTCTC TGATGAGGAG
    GGCAGTTCGG AAGATCTGCT AGAGCTGTCA GATGTGGACA GTGAATCATC GGATGTTAGC
    CAGCCGTACA TTGTATGCAG GCAGTGCCCT GAGTACCGGA GGCAGGGTGG GCAGGCCCTC
    CCCTGCCCAG GGCCTAACAG TGAGCCAGGG GTACCACAGG CTGCTGGGGA TGCGCCGTCA
    ACCTCCACCA GTGTCAAGAC AGCCCAGGAT TATGTGTGCG CCCTGCAAGG AAGCCATGCC
    ATATGCACCT GCTGCTTCCA GCCCATGCCT GACCGGAGGG CAGAACGTGA GCAGGACCCC
    CGCATCGCCC CCCAGCAGTG TGCCGTTTGC CTACAGCCTT TCTGCCACCT GTACTGGGGC
    TGCGCCCGGG CGGGCTGCCT TGGCTGCCTG GCCCCATTCT GTGAGCTAGA CCTGGGCGAC
    AGGTGTCTGG ACGGGGTTCT GAGCAACAAC CACTACGAGT CAGACGTCCT GAAGAATTAT
    CTGGCAACCA GGGGTTTGAC ATGGAAAAAT ATGTTGACAG AAAGTCTGGT GGCTTTGCAG
    AGGGGAGCGT TTTTACTATC TGATTACAGA ATCACGGGGA ACACCGTGCT GTGTTACTGC
    TGTGGCCTGC GGAGCTTCCG AGAGCTGACC TACCAGTATC GGCAGAACAT CCCTGCTTCT
    GAGTTGCCAG TGGCTGTAAC ATCCCGTCCT GATTGCTATT GGGGGCGCAA CTGCCGCACT
    CAGGTGAAGG CCCACCACGC AATGAAATTC AATCATATCT GTGAACAGAC AAGGTTCAAG
    AACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_534631.3
    CDS177..2162
    Translation

    Target ORF information:

    RefSeq Version XM_534631.4
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase (CHFR), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_534631.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGAGCAGC GCCGGGAAGG CGAGCAGCCG CCGCAGCAGC AGCCGTGGGG GAGGCTCCTC 
    CGCTTGGGCG CCGGGGACGG CGAGCCGCAC GTCCTCCTGA GGAACCGGGA GTGGACCATC
    GGGCGGAGGA GAGGTTGTGA CCTTTCATTC CCTGGCAATA AACTGGTCTC TGGAGATCAC
    TGTAAAATTA TAGTGGATGA AAAATCTGGT CAGGTGTCAC TAGAAGATAC CAGCACCAAT
    GGAACAGTAA TTAACAAGCT GAAGGTTGTT AAGAAGCAGA CCTGCCCTTT GCAGACGGGG
    GATGTCATCT ACTTGGTGTA CAGGAAGAAT GAGCCAGAAC ACAATGTGGC ATACCTCTAT
    GAATCTTTAA ATGAGAAGCA GGACATCACA CAAGACTCCT TTGAAGCCAA TAAAGAGAAT
    GTGTTCCGTG TGACCAAAGA TACCTCAGGT GCAGGGCAAG GTGACGACCC CCAGGTCCTG
    CCGTCATCGC CCACCACTCA GGCGTGCTTT GAGGAACCAC AGCCATCAAC ATCCACATCA
    GACCTCTTCC CCACGGCTTC TACCTCTTCC ATGGAGCCCA CCTCTGCAGG GCAAGCACCT
    TCCTCCAGTT CTGCTTCCAG AGATGCATAC ATCTCCCCAA AAGAGTGTGG TCCATCTGTG
    GCAAGTGATG AAATCCCCAT CTTTCCTTCA GCTCTGCCAG ACAGAGAGAG AGCATCCTTT
    TCTCTGTCGG AACCCCAGGA TCAGGAGGAT TTGGAGCCCA TCAGGAAGAA AATGAAAGGA
    GGTGGGGACC CTGACCTTAC CCTGCAGTTC TTGGTTGCAG ACCAGTGTAG AGACACCCAC
    ACCACCCTTG GGAATGTTAG ATCCGAGGCT GTGAAGCCTG ACAAGATGGA GGAGACACTC
    ACATGCATCA TCTGCCAGGA CCTGCTGCAT GATTGTGTGA GCCTGCAGCC CTGCATGCAC
    ACCTTCTGTG CAGCCTGCTA CTCAGGCTGG ATGGAGCGTT CTACCTGGTG TCCCACCTGC
    CGCTGTCCAG TCGAGAGGAT CTGTAAAAAC CACATCCTGA ACAACCTGGT GGAGGCCTAC
    CTTCTCCAGC ACCCAGACAA GAGTCGCAGT GAGGAAGATG TGCGGAGCAT GGCTGCCAGG
    AACAAGATCA CTCAAGACAT GTTGCAACCC AAGGTCAGAA GGTCTTTCTC TGATGAGGAG
    GGCAGTTCGG AAGATCTGCT AGAGCTGTCA GATGTGGACA GTGAATCATC GGATGTTAGC
    CAGCCGTACA TTGTATGCAG GCAGTGCCCT GAGTACCGGA GGCAGGGTGG GCAGGCCCTC
    CCCTGCCCAG GGCCTAACAG TGAGCCAGGG GTACCACAGG CTGCTGGGGA TGCGCCGTCA
    ACCTCCACCA GTGTCAAGAC AGCCCAGGAT TATGTGTGCG CCCTGCAAGG AAGCCATGCC
    ATATGCACCT GCTGCTTCCA GCCCATGCCT GACCGGAGGG CAGAACGTGA GCAGGACCCC
    CGCATCGCCC CCCAGCAGTG TGCCGTTTGC CTACAGCCTT TCTGCCACCT GTACTGGGGC
    TGCGCCCGGG CGGGCTGCCT TGGCTGCCTG GCCCCATTCT GTGAGCTAGA CCTGGGCGAC
    AGGTGTCTGG ACGGGGTTCT GAGCAACAAC CACTACGAGT CAGACGTCCT GAAGAATTAT
    CTGGCAACCA GGGGTTTGAC ATGGAAAAAT ATGTTGACAG AAAGTCTGGT GGCTTTGCAG
    AGGGGAGCGT TTTTACTATC TGATTACAGA ATCACGGGGA ACACCGTGCT GTGTTACTGC
    TGTGGCCTGC GGAGCTTCCG AGAGCTGACC TACCAGTATC GGCAGAACAT CCCTGCTTCT
    GAGTTGCCAG TGGCTGTAAC ATCCCGTCCT GATTGCTATT GGGGGCGCAA CTGCCGCACT
    CAGGTGAAGG CCCACCACGC AATGAAATTC AATCATATCT GTGAACAGAC AAGGTTCAAG
    AACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe26048
    Clone ID Related Accession (Same CDS sequence) XM_534631.5 , XM_534631.4 , XM_022410413.1 , XM_014107998.1 , XM_005636063.2 , XM_022410412.1
    Accession Version XM_022410413.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1986bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product E3 ubiquitin-protein ligase CHFR isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006608.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGAGCAGC GCCGGGAAGG CGAGCAGCCG CCGCAGCAGC AGCCGTGGGG GAGGCTCCTC 
    CGCTTGGGCG CCGGGGACGG CGAGCCGCAC GTCCTCCTGA GGAACCGGGA GTGGACCATC
    GGGCGGAGGA GAGGTTGTGA CCTTTCATTC CCTGGCAATA AACTGGTCTC TGGAGATCAC
    TGTAAAATTA TAGTGGATGA AAAATCTGGT CAGGTGTCAC TAGAAGATAC CAGCACCAAT
    GGAACAGTAA TTAACAAGCT GAAGGTTGTT AAGAAGCAGA CCTGCCCTTT GCAGACGGGG
    GATGTCATCT ACTTGGTGTA CAGGAAGAAT GAGCCAGAAC ACAATGTGGC ATACCTCTAT
    GAATCTTTAA ATGAGAAGCA GGACATCACA CAAGACTCCT TTGAAGCCAA TAAAGAGAAT
    GTGTTCCGTG TGACCAAAGA TACCTCAGGT GCAGGGCAAG GTGACGACCC CCAGGTCCTG
    CCGTCATCGC CCACCACTCA GGCGTGCTTT GAGGAACCAC AGCCATCAAC ATCCACATCA
    GACCTCTTCC CCACGGCTTC TACCTCTTCC ATGGAGCCCA CCTCTGCAGG GCAAGCACCT
    TCCTCCAGTT CTGCTTCCAG AGATGCATAC ATCTCCCCAA AAGAGTGTGG TCCATCTGTG
    GCAAGTGATG AAATCCCCAT CTTTCCTTCA GCTCTGCCAG ACAGAGAGAG AGCATCCTTT
    TCTCTGTCGG AACCCCAGGA TCAGGAGGAT TTGGAGCCCA TCAGGAAGAA AATGAAAGGA
    GGTGGGGACC CTGACCTTAC CCTGCAGTTC TTGGTTGCAG ACCAGTGTAG AGACACCCAC
    ACCACCCTTG GGAATGTTAG ATCCGAGGCT GTGAAGCCTG ACAAGATGGA GGAGACACTC
    ACATGCATCA TCTGCCAGGA CCTGCTGCAT GATTGTGTGA GCCTGCAGCC CTGCATGCAC
    ACCTTCTGTG CAGCCTGCTA CTCAGGCTGG ATGGAGCGTT CTACCTGGTG TCCCACCTGC
    CGCTGTCCAG TCGAGAGGAT CTGTAAAAAC CACATCCTGA ACAACCTGGT GGAGGCCTAC
    CTTCTCCAGC ACCCAGACAA GAGTCGCAGT GAGGAAGATG TGCGGAGCAT GGCTGCCAGG
    AACAAGATCA CTCAAGACAT GTTGCAACCC AAGGTCAGAA GGTCTTTCTC TGATGAGGAG
    GGCAGTTCGG AAGATCTGCT AGAGCTGTCA GATGTGGACA GTGAATCATC GGATGTTAGC
    CAGCCGTACA TTGTATGCAG GCAGTGCCCT GAGTACCGGA GGCAGGGTGG GCAGGCCCTC
    CCCTGCCCAG GGCCTAACAG TGAGCCAGGG GTACCACAGG CTGCTGGGGA TGCGCCGTCA
    ACCTCCACCA GTGTCAAGAC AGCCCAGGAT TATGTGTGCG CCCTGCAAGG AAGCCATGCC
    ATATGCACCT GCTGCTTCCA GCCCATGCCT GACCGGAGGG CAGAACGTGA GCAGGACCCC
    CGCATCGCCC CCCAGCAGTG TGCCGTTTGC CTACAGCCTT TCTGCCACCT GTACTGGGGC
    TGCGCCCGGG CGGGCTGCCT TGGCTGCCTG GCCCCATTCT GTGAGCTAGA CCTGGGCGAC
    AGGTGTCTGG ACGGGGTTCT GAGCAACAAC CACTACGAGT CAGACGTCCT GAAGAATTAT
    CTGGCAACCA GGGGTTTGAC ATGGAAAAAT ATGTTGACAG AAAGTCTGGT GGCTTTGCAG
    AGGGGAGCGT TTTTACTATC TGATTACAGA ATCACGGGGA ACACCGTGCT GTGTTACTGC
    TGTGGCCTGC GGAGCTTCCG AGAGCTGACC TACCAGTATC GGCAGAACAT CCCTGCTTCT
    GAGTTGCCAG TGGCTGTAAC ATCCCGTCCT GATTGCTATT GGGGGCGCAA CTGCCGCACT
    CAGGTGAAGG CCCACCACGC AATGAAATTC AATCATATCT GTGAACAGAC AAGGTTCAAG
    AACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022266121.1
    CDS12..1997
    Translation

    Target ORF information:

    RefSeq Version XM_022410413.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris checkpoint with forkhead and ring finger domains (CHFR), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022410413.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGAGCAGC GCCGGGAAGG CGAGCAGCCG CCGCAGCAGC AGCCGTGGGG GAGGCTCCTC 
    CGCTTGGGCG CCGGGGACGG CGAGCCGCAC GTCCTCCTGA GGAACCGGGA GTGGACCATC
    GGGCGGAGGA GAGGTTGTGA CCTTTCATTC CCTGGCAATA AACTGGTCTC TGGAGATCAC
    TGTAAAATTA TAGTGGATGA AAAATCTGGT CAGGTGTCAC TAGAAGATAC CAGCACCAAT
    GGAACAGTAA TTAACAAGCT GAAGGTTGTT AAGAAGCAGA CCTGCCCTTT GCAGACGGGG
    GATGTCATCT ACTTGGTGTA CAGGAAGAAT GAGCCAGAAC ACAATGTGGC ATACCTCTAT
    GAATCTTTAA ATGAGAAGCA GGACATCACA CAAGACTCCT TTGAAGCCAA TAAAGAGAAT
    GTGTTCCGTG TGACCAAAGA TACCTCAGGT GCAGGGCAAG GTGACGACCC CCAGGTCCTG
    CCGTCATCGC CCACCACTCA GGCGTGCTTT GAGGAACCAC AGCCATCAAC ATCCACATCA
    GACCTCTTCC CCACGGCTTC TACCTCTTCC ATGGAGCCCA CCTCTGCAGG GCAAGCACCT
    TCCTCCAGTT CTGCTTCCAG AGATGCATAC ATCTCCCCAA AAGAGTGTGG TCCATCTGTG
    GCAAGTGATG AAATCCCCAT CTTTCCTTCA GCTCTGCCAG ACAGAGAGAG AGCATCCTTT
    TCTCTGTCGG AACCCCAGGA TCAGGAGGAT TTGGAGCCCA TCAGGAAGAA AATGAAAGGA
    GGTGGGGACC CTGACCTTAC CCTGCAGTTC TTGGTTGCAG ACCAGTGTAG AGACACCCAC
    ACCACCCTTG GGAATGTTAG ATCCGAGGCT GTGAAGCCTG ACAAGATGGA GGAGACACTC
    ACATGCATCA TCTGCCAGGA CCTGCTGCAT GATTGTGTGA GCCTGCAGCC CTGCATGCAC
    ACCTTCTGTG CAGCCTGCTA CTCAGGCTGG ATGGAGCGTT CTACCTGGTG TCCCACCTGC
    CGCTGTCCAG TCGAGAGGAT CTGTAAAAAC CACATCCTGA ACAACCTGGT GGAGGCCTAC
    CTTCTCCAGC ACCCAGACAA GAGTCGCAGT GAGGAAGATG TGCGGAGCAT GGCTGCCAGG
    AACAAGATCA CTCAAGACAT GTTGCAACCC AAGGTCAGAA GGTCTTTCTC TGATGAGGAG
    GGCAGTTCGG AAGATCTGCT AGAGCTGTCA GATGTGGACA GTGAATCATC GGATGTTAGC
    CAGCCGTACA TTGTATGCAG GCAGTGCCCT GAGTACCGGA GGCAGGGTGG GCAGGCCCTC
    CCCTGCCCAG GGCCTAACAG TGAGCCAGGG GTACCACAGG CTGCTGGGGA TGCGCCGTCA
    ACCTCCACCA GTGTCAAGAC AGCCCAGGAT TATGTGTGCG CCCTGCAAGG AAGCCATGCC
    ATATGCACCT GCTGCTTCCA GCCCATGCCT GACCGGAGGG CAGAACGTGA GCAGGACCCC
    CGCATCGCCC CCCAGCAGTG TGCCGTTTGC CTACAGCCTT TCTGCCACCT GTACTGGGGC
    TGCGCCCGGG CGGGCTGCCT TGGCTGCCTG GCCCCATTCT GTGAGCTAGA CCTGGGCGAC
    AGGTGTCTGG ACGGGGTTCT GAGCAACAAC CACTACGAGT CAGACGTCCT GAAGAATTAT
    CTGGCAACCA GGGGTTTGAC ATGGAAAAAT ATGTTGACAG AAAGTCTGGT GGCTTTGCAG
    AGGGGAGCGT TTTTACTATC TGATTACAGA ATCACGGGGA ACACCGTGCT GTGTTACTGC
    TGTGGCCTGC GGAGCTTCCG AGAGCTGACC TACCAGTATC GGCAGAACAT CCCTGCTTCT
    GAGTTGCCAG TGGCTGTAAC ATCCCGTCCT GATTGCTATT GGGGGCGCAA CTGCCGCACT
    CAGGTGAAGG CCCACCACGC AATGAAATTC AATCATATCT GTGAACAGAC AAGGTTCAAG
    AACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe26048
    Clone ID Related Accession (Same CDS sequence) XM_534631.5 , XM_534631.4 , XM_022410413.1 , XM_014107998.1 , XM_005636063.2 , XM_022410412.1
    Accession Version XM_014107998.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1986bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product E3 ubiquitin-protein ligase CHFR isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726106.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGAGCAGC GCCGGGAAGG CGAGCAGCCG CCGCAGCAGC AGCCGTGGGG GAGGCTCCTC 
    CGCTTGGGCG CCGGGGACGG CGAGCCGCAC GTCCTCCTGA GGAACCGGGA GTGGACCATC
    GGGCGGAGGA GAGGTTGTGA CCTTTCATTC CCTGGCAATA AACTGGTCTC TGGAGATCAC
    TGTAAAATTA TAGTGGATGA AAAATCTGGT CAGGTGTCAC TAGAAGATAC CAGCACCAAT
    GGAACAGTAA TTAACAAGCT GAAGGTTGTT AAGAAGCAGA CCTGCCCTTT GCAGACGGGG
    GATGTCATCT ACTTGGTGTA CAGGAAGAAT GAGCCAGAAC ACAATGTGGC ATACCTCTAT
    GAATCTTTAA ATGAGAAGCA GGACATCACA CAAGACTCCT TTGAAGCCAA TAAAGAGAAT
    GTGTTCCGTG TGACCAAAGA TACCTCAGGT GCAGGGCAAG GTGACGACCC CCAGGTCCTG
    CCGTCATCGC CCACCACTCA GGCGTGCTTT GAGGAACCAC AGCCATCAAC ATCCACATCA
    GACCTCTTCC CCACGGCTTC TACCTCTTCC ATGGAGCCCA CCTCTGCAGG GCAAGCACCT
    TCCTCCAGTT CTGCTTCCAG AGATGCATAC ATCTCCCCAA AAGAGTGTGG TCCATCTGTG
    GCAAGTGATG AAATCCCCAT CTTTCCTTCA GCTCTGCCAG ACAGAGAGAG AGCATCCTTT
    TCTCTGTCGG AACCCCAGGA TCAGGAGGAT TTGGAGCCCA TCAGGAAGAA AATGAAAGGA
    GGTGGGGACC CTGACCTTAC CCTGCAGTTC TTGGTTGCAG ACCAGTGTAG AGACACCCAC
    ACCACCCTTG GGAATGTTAG ATCCGAGGCT GTGAAGCCTG ACAAGATGGA GGAGACACTC
    ACATGCATCA TCTGCCAGGA CCTGCTGCAT GATTGTGTGA GCCTGCAGCC CTGCATGCAC
    ACCTTCTGTG CAGCCTGCTA CTCAGGCTGG ATGGAGCGTT CTACCTGGTG TCCCACCTGC
    CGCTGTCCAG TCGAGAGGAT CTGTAAAAAC CACATCCTGA ACAACCTGGT GGAGGCCTAC
    CTTCTCCAGC ACCCAGACAA GAGTCGCAGT GAGGAAGATG TGCGGAGCAT GGCTGCCAGG
    AACAAGATCA CTCAAGACAT GTTGCAACCC AAGGTCAGAA GGTCTTTCTC TGATGAGGAG
    GGCAGTTCGG AAGATCTGCT AGAGCTGTCA GATGTGGACA GTGAATCATC GGATGTTAGC
    CAGCCGTACA TTGTATGCAG GCAGTGCCCT GAGTACCGGA GGCAGGGTGG GCAGGCCCTC
    CCCTGCCCAG GGCCTAACAG TGAGCCAGGG GTACCACAGG CTGCTGGGGA TGCGCCGTCA
    ACCTCCACCA GTGTCAAGAC AGCCCAGGAT TATGTGTGCG CCCTGCAAGG AAGCCATGCC
    ATATGCACCT GCTGCTTCCA GCCCATGCCT GACCGGAGGG CAGAACGTGA GCAGGACCCC
    CGCATCGCCC CCCAGCAGTG TGCCGTTTGC CTACAGCCTT TCTGCCACCT GTACTGGGGC
    TGCGCCCGGG CGGGCTGCCT TGGCTGCCTG GCCCCATTCT GTGAGCTAGA CCTGGGCGAC
    AGGTGTCTGG ACGGGGTTCT GAGCAACAAC CACTACGAGT CAGACGTCCT GAAGAATTAT
    CTGGCAACCA GGGGTTTGAC ATGGAAAAAT ATGTTGACAG AAAGTCTGGT GGCTTTGCAG
    AGGGGAGCGT TTTTACTATC TGATTACAGA ATCACGGGGA ACACCGTGCT GTGTTACTGC
    TGTGGCCTGC GGAGCTTCCG AGAGCTGACC TACCAGTATC GGCAGAACAT CCCTGCTTCT
    GAGTTGCCAG TGGCTGTAAC ATCCCGTCCT GATTGCTATT GGGGGCGCAA CTGCCGCACT
    CAGGTGAAGG CCCACCACGC AATGAAATTC AATCATATCT GTGAACAGAC AAGGTTCAAG
    AACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013963473.1
    CDS99..2084
    Translation

    Target ORF information:

    RefSeq Version XM_014107998.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase (CHFR), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014107998.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGAGCAGC GCCGGGAAGG CGAGCAGCCG CCGCAGCAGC AGCCGTGGGG GAGGCTCCTC 
    CGCTTGGGCG CCGGGGACGG CGAGCCGCAC GTCCTCCTGA GGAACCGGGA GTGGACCATC
    GGGCGGAGGA GAGGTTGTGA CCTTTCATTC CCTGGCAATA AACTGGTCTC TGGAGATCAC
    TGTAAAATTA TAGTGGATGA AAAATCTGGT CAGGTGTCAC TAGAAGATAC CAGCACCAAT
    GGAACAGTAA TTAACAAGCT GAAGGTTGTT AAGAAGCAGA CCTGCCCTTT GCAGACGGGG
    GATGTCATCT ACTTGGTGTA CAGGAAGAAT GAGCCAGAAC ACAATGTGGC ATACCTCTAT
    GAATCTTTAA ATGAGAAGCA GGACATCACA CAAGACTCCT TTGAAGCCAA TAAAGAGAAT
    GTGTTCCGTG TGACCAAAGA TACCTCAGGT GCAGGGCAAG GTGACGACCC CCAGGTCCTG
    CCGTCATCGC CCACCACTCA GGCGTGCTTT GAGGAACCAC AGCCATCAAC ATCCACATCA
    GACCTCTTCC CCACGGCTTC TACCTCTTCC ATGGAGCCCA CCTCTGCAGG GCAAGCACCT
    TCCTCCAGTT CTGCTTCCAG AGATGCATAC ATCTCCCCAA AAGAGTGTGG TCCATCTGTG
    GCAAGTGATG AAATCCCCAT CTTTCCTTCA GCTCTGCCAG ACAGAGAGAG AGCATCCTTT
    TCTCTGTCGG AACCCCAGGA TCAGGAGGAT TTGGAGCCCA TCAGGAAGAA AATGAAAGGA
    GGTGGGGACC CTGACCTTAC CCTGCAGTTC TTGGTTGCAG ACCAGTGTAG AGACACCCAC
    ACCACCCTTG GGAATGTTAG ATCCGAGGCT GTGAAGCCTG ACAAGATGGA GGAGACACTC
    ACATGCATCA TCTGCCAGGA CCTGCTGCAT GATTGTGTGA GCCTGCAGCC CTGCATGCAC
    ACCTTCTGTG CAGCCTGCTA CTCAGGCTGG ATGGAGCGTT CTACCTGGTG TCCCACCTGC
    CGCTGTCCAG TCGAGAGGAT CTGTAAAAAC CACATCCTGA ACAACCTGGT GGAGGCCTAC
    CTTCTCCAGC ACCCAGACAA GAGTCGCAGT GAGGAAGATG TGCGGAGCAT GGCTGCCAGG
    AACAAGATCA CTCAAGACAT GTTGCAACCC AAGGTCAGAA GGTCTTTCTC TGATGAGGAG
    GGCAGTTCGG AAGATCTGCT AGAGCTGTCA GATGTGGACA GTGAATCATC GGATGTTAGC
    CAGCCGTACA TTGTATGCAG GCAGTGCCCT GAGTACCGGA GGCAGGGTGG GCAGGCCCTC
    CCCTGCCCAG GGCCTAACAG TGAGCCAGGG GTACCACAGG CTGCTGGGGA TGCGCCGTCA
    ACCTCCACCA GTGTCAAGAC AGCCCAGGAT TATGTGTGCG CCCTGCAAGG AAGCCATGCC
    ATATGCACCT GCTGCTTCCA GCCCATGCCT GACCGGAGGG CAGAACGTGA GCAGGACCCC
    CGCATCGCCC CCCAGCAGTG TGCCGTTTGC CTACAGCCTT TCTGCCACCT GTACTGGGGC
    TGCGCCCGGG CGGGCTGCCT TGGCTGCCTG GCCCCATTCT GTGAGCTAGA CCTGGGCGAC
    AGGTGTCTGG ACGGGGTTCT GAGCAACAAC CACTACGAGT CAGACGTCCT GAAGAATTAT
    CTGGCAACCA GGGGTTTGAC ATGGAAAAAT ATGTTGACAG AAAGTCTGGT GGCTTTGCAG
    AGGGGAGCGT TTTTACTATC TGATTACAGA ATCACGGGGA ACACCGTGCT GTGTTACTGC
    TGTGGCCTGC GGAGCTTCCG AGAGCTGACC TACCAGTATC GGCAGAACAT CCCTGCTTCT
    GAGTTGCCAG TGGCTGTAAC ATCCCGTCCT GATTGCTATT GGGGGCGCAA CTGCCGCACT
    CAGGTGAAGG CCCACCACGC AATGAAATTC AATCATATCT GTGAACAGAC AAGGTTCAAG
    AACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe26048
    Clone ID Related Accession (Same CDS sequence) XM_534631.5 , XM_534631.4 , XM_022410413.1 , XM_014107998.1 , XM_005636063.2 , XM_022410412.1
    Accession Version XM_005636063.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1986bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product E3 ubiquitin-protein ligase CHFR isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726106.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005636063.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGAGCAGC GCCGGGAAGG CGAGCAGCCG CCGCAGCAGC AGCCGTGGGG GAGGCTCCTC 
    CGCTTGGGCG CCGGGGACGG CGAGCCGCAC GTCCTCCTGA GGAACCGGGA GTGGACCATC
    GGGCGGAGGA GAGGTTGTGA CCTTTCATTC CCTGGCAATA AACTGGTCTC TGGAGATCAC
    TGTAAAATTA TAGTGGATGA AAAATCTGGT CAGGTGTCAC TAGAAGATAC CAGCACCAAT
    GGAACAGTAA TTAACAAGCT GAAGGTTGTT AAGAAGCAGA CCTGCCCTTT GCAGACGGGG
    GATGTCATCT ACTTGGTGTA CAGGAAGAAT GAGCCAGAAC ACAATGTGGC ATACCTCTAT
    GAATCTTTAA ATGAGAAGCA GGACATCACA CAAGACTCCT TTGAAGCCAA TAAAGAGAAT
    GTGTTCCGTG TGACCAAAGA TACCTCAGGT GCAGGGCAAG GTGACGACCC CCAGGTCCTG
    CCGTCATCGC CCACCACTCA GGCGTGCTTT GAGGAACCAC AGCCATCAAC ATCCACATCA
    GACCTCTTCC CCACGGCTTC TACCTCTTCC ATGGAGCCCA CCTCTGCAGG GCAAGCACCT
    TCCTCCAGTT CTGCTTCCAG AGATGCATAC ATCTCCCCAA AAGAGTGTGG TCCATCTGTG
    GCAAGTGATG AAATCCCCAT CTTTCCTTCA GCTCTGCCAG ACAGAGAGAG AGCATCCTTT
    TCTCTGTCGG AACCCCAGGA TCAGGAGGAT TTGGAGCCCA TCAGGAAGAA AATGAAAGGA
    GGTGGGGACC CTGACCTTAC CCTGCAGTTC TTGGTTGCAG ACCAGTGTAG AGACACCCAC
    ACCACCCTTG GGAATGTTAG ATCCGAGGCT GTGAAGCCTG ACAAGATGGA GGAGACACTC
    ACATGCATCA TCTGCCAGGA CCTGCTGCAT GATTGTGTGA GCCTGCAGCC CTGCATGCAC
    ACCTTCTGTG CAGCCTGCTA CTCAGGCTGG ATGGAGCGTT CTACCTGGTG TCCCACCTGC
    CGCTGTCCAG TCGAGAGGAT CTGTAAAAAC CACATCCTGA ACAACCTGGT GGAGGCCTAC
    CTTCTCCAGC ACCCAGACAA GAGTCGCAGT GAGGAAGATG TGCGGAGCAT GGCTGCCAGG
    AACAAGATCA CTCAAGACAT GTTGCAACCC AAGGTCAGAA GGTCTTTCTC TGATGAGGAG
    GGCAGTTCGG AAGATCTGCT AGAGCTGTCA GATGTGGACA GTGAATCATC GGATGTTAGC
    CAGCCGTACA TTGTATGCAG GCAGTGCCCT GAGTACCGGA GGCAGGGTGG GCAGGCCCTC
    CCCTGCCCAG GGCCTAACAG TGAGCCAGGG GTACCACAGG CTGCTGGGGA TGCGCCGTCA
    ACCTCCACCA GTGTCAAGAC AGCCCAGGAT TATGTGTGCG CCCTGCAAGG AAGCCATGCC
    ATATGCACCT GCTGCTTCCA GCCCATGCCT GACCGGAGGG CAGAACGTGA GCAGGACCCC
    CGCATCGCCC CCCAGCAGTG TGCCGTTTGC CTACAGCCTT TCTGCCACCT GTACTGGGGC
    TGCGCCCGGG CGGGCTGCCT TGGCTGCCTG GCCCCATTCT GTGAGCTAGA CCTGGGCGAC
    AGGTGTCTGG ACGGGGTTCT GAGCAACAAC CACTACGAGT CAGACGTCCT GAAGAATTAT
    CTGGCAACCA GGGGTTTGAC ATGGAAAAAT ATGTTGACAG AAAGTCTGGT GGCTTTGCAG
    AGGGGAGCGT TTTTACTATC TGATTACAGA ATCACGGGGA ACACCGTGCT GTGTTACTGC
    TGTGGCCTGC GGAGCTTCCG AGAGCTGACC TACCAGTATC GGCAGAACAT CCCTGCTTCT
    GAGTTGCCAG TGGCTGTAAC ATCCCGTCCT GATTGCTATT GGGGGCGCAA CTGCCGCACT
    CAGGTGAAGG CCCACCACGC AATGAAATTC AATCATATCT GTGAACAGAC AAGGTTCAAG
    AACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005636120.1
    CDS178..2163
    Translation

    Target ORF information:

    RefSeq Version XM_005636063.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase (CHFR), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005636063.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGAGCAGC GCCGGGAAGG CGAGCAGCCG CCGCAGCAGC AGCCGTGGGG GAGGCTCCTC 
    CGCTTGGGCG CCGGGGACGG CGAGCCGCAC GTCCTCCTGA GGAACCGGGA GTGGACCATC
    GGGCGGAGGA GAGGTTGTGA CCTTTCATTC CCTGGCAATA AACTGGTCTC TGGAGATCAC
    TGTAAAATTA TAGTGGATGA AAAATCTGGT CAGGTGTCAC TAGAAGATAC CAGCACCAAT
    GGAACAGTAA TTAACAAGCT GAAGGTTGTT AAGAAGCAGA CCTGCCCTTT GCAGACGGGG
    GATGTCATCT ACTTGGTGTA CAGGAAGAAT GAGCCAGAAC ACAATGTGGC ATACCTCTAT
    GAATCTTTAA ATGAGAAGCA GGACATCACA CAAGACTCCT TTGAAGCCAA TAAAGAGAAT
    GTGTTCCGTG TGACCAAAGA TACCTCAGGT GCAGGGCAAG GTGACGACCC CCAGGTCCTG
    CCGTCATCGC CCACCACTCA GGCGTGCTTT GAGGAACCAC AGCCATCAAC ATCCACATCA
    GACCTCTTCC CCACGGCTTC TACCTCTTCC ATGGAGCCCA CCTCTGCAGG GCAAGCACCT
    TCCTCCAGTT CTGCTTCCAG AGATGCATAC ATCTCCCCAA AAGAGTGTGG TCCATCTGTG
    GCAAGTGATG AAATCCCCAT CTTTCCTTCA GCTCTGCCAG ACAGAGAGAG AGCATCCTTT
    TCTCTGTCGG AACCCCAGGA TCAGGAGGAT TTGGAGCCCA TCAGGAAGAA AATGAAAGGA
    GGTGGGGACC CTGACCTTAC CCTGCAGTTC TTGGTTGCAG ACCAGTGTAG AGACACCCAC
    ACCACCCTTG GGAATGTTAG ATCCGAGGCT GTGAAGCCTG ACAAGATGGA GGAGACACTC
    ACATGCATCA TCTGCCAGGA CCTGCTGCAT GATTGTGTGA GCCTGCAGCC CTGCATGCAC
    ACCTTCTGTG CAGCCTGCTA CTCAGGCTGG ATGGAGCGTT CTACCTGGTG TCCCACCTGC
    CGCTGTCCAG TCGAGAGGAT CTGTAAAAAC CACATCCTGA ACAACCTGGT GGAGGCCTAC
    CTTCTCCAGC ACCCAGACAA GAGTCGCAGT GAGGAAGATG TGCGGAGCAT GGCTGCCAGG
    AACAAGATCA CTCAAGACAT GTTGCAACCC AAGGTCAGAA GGTCTTTCTC TGATGAGGAG
    GGCAGTTCGG AAGATCTGCT AGAGCTGTCA GATGTGGACA GTGAATCATC GGATGTTAGC
    CAGCCGTACA TTGTATGCAG GCAGTGCCCT GAGTACCGGA GGCAGGGTGG GCAGGCCCTC
    CCCTGCCCAG GGCCTAACAG TGAGCCAGGG GTACCACAGG CTGCTGGGGA TGCGCCGTCA
    ACCTCCACCA GTGTCAAGAC AGCCCAGGAT TATGTGTGCG CCCTGCAAGG AAGCCATGCC
    ATATGCACCT GCTGCTTCCA GCCCATGCCT GACCGGAGGG CAGAACGTGA GCAGGACCCC
    CGCATCGCCC CCCAGCAGTG TGCCGTTTGC CTACAGCCTT TCTGCCACCT GTACTGGGGC
    TGCGCCCGGG CGGGCTGCCT TGGCTGCCTG GCCCCATTCT GTGAGCTAGA CCTGGGCGAC
    AGGTGTCTGG ACGGGGTTCT GAGCAACAAC CACTACGAGT CAGACGTCCT GAAGAATTAT
    CTGGCAACCA GGGGTTTGAC ATGGAAAAAT ATGTTGACAG AAAGTCTGGT GGCTTTGCAG
    AGGGGAGCGT TTTTACTATC TGATTACAGA ATCACGGGGA ACACCGTGCT GTGTTACTGC
    TGTGGCCTGC GGAGCTTCCG AGAGCTGACC TACCAGTATC GGCAGAACAT CCCTGCTTCT
    GAGTTGCCAG TGGCTGTAAC ATCCCGTCCT GATTGCTATT GGGGGCGCAA CTGCCGCACT
    CAGGTGAAGG CCCACCACGC AATGAAATTC AATCATATCT GTGAACAGAC AAGGTTCAAG
    AACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe26048
    Clone ID Related Accession (Same CDS sequence) XM_534631.5 , XM_534631.4 , XM_022410413.1 , XM_014107998.1 , XM_005636063.2 , XM_022410412.1
    Accession Version XM_022410412.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1986bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product E3 ubiquitin-protein ligase CHFR isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006608.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGAGCAGC GCCGGGAAGG CGAGCAGCCG CCGCAGCAGC AGCCGTGGGG GAGGCTCCTC 
    CGCTTGGGCG CCGGGGACGG CGAGCCGCAC GTCCTCCTGA GGAACCGGGA GTGGACCATC
    GGGCGGAGGA GAGGTTGTGA CCTTTCATTC CCTGGCAATA AACTGGTCTC TGGAGATCAC
    TGTAAAATTA TAGTGGATGA AAAATCTGGT CAGGTGTCAC TAGAAGATAC CAGCACCAAT
    GGAACAGTAA TTAACAAGCT GAAGGTTGTT AAGAAGCAGA CCTGCCCTTT GCAGACGGGG
    GATGTCATCT ACTTGGTGTA CAGGAAGAAT GAGCCAGAAC ACAATGTGGC ATACCTCTAT
    GAATCTTTAA ATGAGAAGCA GGACATCACA CAAGACTCCT TTGAAGCCAA TAAAGAGAAT
    GTGTTCCGTG TGACCAAAGA TACCTCAGGT GCAGGGCAAG GTGACGACCC CCAGGTCCTG
    CCGTCATCGC CCACCACTCA GGCGTGCTTT GAGGAACCAC AGCCATCAAC ATCCACATCA
    GACCTCTTCC CCACGGCTTC TACCTCTTCC ATGGAGCCCA CCTCTGCAGG GCAAGCACCT
    TCCTCCAGTT CTGCTTCCAG AGATGCATAC ATCTCCCCAA AAGAGTGTGG TCCATCTGTG
    GCAAGTGATG AAATCCCCAT CTTTCCTTCA GCTCTGCCAG ACAGAGAGAG AGCATCCTTT
    TCTCTGTCGG AACCCCAGGA TCAGGAGGAT TTGGAGCCCA TCAGGAAGAA AATGAAAGGA
    GGTGGGGACC CTGACCTTAC CCTGCAGTTC TTGGTTGCAG ACCAGTGTAG AGACACCCAC
    ACCACCCTTG GGAATGTTAG ATCCGAGGCT GTGAAGCCTG ACAAGATGGA GGAGACACTC
    ACATGCATCA TCTGCCAGGA CCTGCTGCAT GATTGTGTGA GCCTGCAGCC CTGCATGCAC
    ACCTTCTGTG CAGCCTGCTA CTCAGGCTGG ATGGAGCGTT CTACCTGGTG TCCCACCTGC
    CGCTGTCCAG TCGAGAGGAT CTGTAAAAAC CACATCCTGA ACAACCTGGT GGAGGCCTAC
    CTTCTCCAGC ACCCAGACAA GAGTCGCAGT GAGGAAGATG TGCGGAGCAT GGCTGCCAGG
    AACAAGATCA CTCAAGACAT GTTGCAACCC AAGGTCAGAA GGTCTTTCTC TGATGAGGAG
    GGCAGTTCGG AAGATCTGCT AGAGCTGTCA GATGTGGACA GTGAATCATC GGATGTTAGC
    CAGCCGTACA TTGTATGCAG GCAGTGCCCT GAGTACCGGA GGCAGGGTGG GCAGGCCCTC
    CCCTGCCCAG GGCCTAACAG TGAGCCAGGG GTACCACAGG CTGCTGGGGA TGCGCCGTCA
    ACCTCCACCA GTGTCAAGAC AGCCCAGGAT TATGTGTGCG CCCTGCAAGG AAGCCATGCC
    ATATGCACCT GCTGCTTCCA GCCCATGCCT GACCGGAGGG CAGAACGTGA GCAGGACCCC
    CGCATCGCCC CCCAGCAGTG TGCCGTTTGC CTACAGCCTT TCTGCCACCT GTACTGGGGC
    TGCGCCCGGG CGGGCTGCCT TGGCTGCCTG GCCCCATTCT GTGAGCTAGA CCTGGGCGAC
    AGGTGTCTGG ACGGGGTTCT GAGCAACAAC CACTACGAGT CAGACGTCCT GAAGAATTAT
    CTGGCAACCA GGGGTTTGAC ATGGAAAAAT ATGTTGACAG AAAGTCTGGT GGCTTTGCAG
    AGGGGAGCGT TTTTACTATC TGATTACAGA ATCACGGGGA ACACCGTGCT GTGTTACTGC
    TGTGGCCTGC GGAGCTTCCG AGAGCTGACC TACCAGTATC GGCAGAACAT CCCTGCTTCT
    GAGTTGCCAG TGGCTGTAAC ATCCCGTCCT GATTGCTATT GGGGGCGCAA CTGCCGCACT
    CAGGTGAAGG CCCACCACGC AATGAAATTC AATCATATCT GTGAACAGAC AAGGTTCAAG
    AACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022266120.1
    CDS12..1997
    Translation

    Target ORF information:

    RefSeq Version XM_022410412.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris checkpoint with forkhead and ring finger domains (CHFR), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022410412.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGAGCAGC GCCGGGAAGG CGAGCAGCCG CCGCAGCAGC AGCCGTGGGG GAGGCTCCTC 
    CGCTTGGGCG CCGGGGACGG CGAGCCGCAC GTCCTCCTGA GGAACCGGGA GTGGACCATC
    GGGCGGAGGA GAGGTTGTGA CCTTTCATTC CCTGGCAATA AACTGGTCTC TGGAGATCAC
    TGTAAAATTA TAGTGGATGA AAAATCTGGT CAGGTGTCAC TAGAAGATAC CAGCACCAAT
    GGAACAGTAA TTAACAAGCT GAAGGTTGTT AAGAAGCAGA CCTGCCCTTT GCAGACGGGG
    GATGTCATCT ACTTGGTGTA CAGGAAGAAT GAGCCAGAAC ACAATGTGGC ATACCTCTAT
    GAATCTTTAA ATGAGAAGCA GGACATCACA CAAGACTCCT TTGAAGCCAA TAAAGAGAAT
    GTGTTCCGTG TGACCAAAGA TACCTCAGGT GCAGGGCAAG GTGACGACCC CCAGGTCCTG
    CCGTCATCGC CCACCACTCA GGCGTGCTTT GAGGAACCAC AGCCATCAAC ATCCACATCA
    GACCTCTTCC CCACGGCTTC TACCTCTTCC ATGGAGCCCA CCTCTGCAGG GCAAGCACCT
    TCCTCCAGTT CTGCTTCCAG AGATGCATAC ATCTCCCCAA AAGAGTGTGG TCCATCTGTG
    GCAAGTGATG AAATCCCCAT CTTTCCTTCA GCTCTGCCAG ACAGAGAGAG AGCATCCTTT
    TCTCTGTCGG AACCCCAGGA TCAGGAGGAT TTGGAGCCCA TCAGGAAGAA AATGAAAGGA
    GGTGGGGACC CTGACCTTAC CCTGCAGTTC TTGGTTGCAG ACCAGTGTAG AGACACCCAC
    ACCACCCTTG GGAATGTTAG ATCCGAGGCT GTGAAGCCTG ACAAGATGGA GGAGACACTC
    ACATGCATCA TCTGCCAGGA CCTGCTGCAT GATTGTGTGA GCCTGCAGCC CTGCATGCAC
    ACCTTCTGTG CAGCCTGCTA CTCAGGCTGG ATGGAGCGTT CTACCTGGTG TCCCACCTGC
    CGCTGTCCAG TCGAGAGGAT CTGTAAAAAC CACATCCTGA ACAACCTGGT GGAGGCCTAC
    CTTCTCCAGC ACCCAGACAA GAGTCGCAGT GAGGAAGATG TGCGGAGCAT GGCTGCCAGG
    AACAAGATCA CTCAAGACAT GTTGCAACCC AAGGTCAGAA GGTCTTTCTC TGATGAGGAG
    GGCAGTTCGG AAGATCTGCT AGAGCTGTCA GATGTGGACA GTGAATCATC GGATGTTAGC
    CAGCCGTACA TTGTATGCAG GCAGTGCCCT GAGTACCGGA GGCAGGGTGG GCAGGCCCTC
    CCCTGCCCAG GGCCTAACAG TGAGCCAGGG GTACCACAGG CTGCTGGGGA TGCGCCGTCA
    ACCTCCACCA GTGTCAAGAC AGCCCAGGAT TATGTGTGCG CCCTGCAAGG AAGCCATGCC
    ATATGCACCT GCTGCTTCCA GCCCATGCCT GACCGGAGGG CAGAACGTGA GCAGGACCCC
    CGCATCGCCC CCCAGCAGTG TGCCGTTTGC CTACAGCCTT TCTGCCACCT GTACTGGGGC
    TGCGCCCGGG CGGGCTGCCT TGGCTGCCTG GCCCCATTCT GTGAGCTAGA CCTGGGCGAC
    AGGTGTCTGG ACGGGGTTCT GAGCAACAAC CACTACGAGT CAGACGTCCT GAAGAATTAT
    CTGGCAACCA GGGGTTTGAC ATGGAAAAAT ATGTTGACAG AAAGTCTGGT GGCTTTGCAG
    AGGGGAGCGT TTTTACTATC TGATTACAGA ATCACGGGGA ACACCGTGCT GTGTTACTGC
    TGTGGCCTGC GGAGCTTCCG AGAGCTGACC TACCAGTATC GGCAGAACAT CCCTGCTTCT
    GAGTTGCCAG TGGCTGTAAC ATCCCGTCCT GATTGCTATT GGGGGCGCAA CTGCCGCACT
    CAGGTGAAGG CCCACCACGC AATGAAATTC AATCATATCT GTGAACAGAC AAGGTTCAAG
    AACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe26049
    Clone ID Related Accession (Same CDS sequence) XM_005636065.1 , XM_022410416.1
    Accession Version XM_005636065.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1818bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product E3 ubiquitin-protein ligase CHFR isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726106.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGGAGCAGC GCCGGGAAGG CGAGCAGCCG CCGCAGCAGC AGCCGTGGGG GAGGCTCCTC 
    CGCTTGGGCG CCGGGGACGG CGAGCCGCAC GTCCTCCTGA GGAACCGGGA GTGGACCATC
    GGGCGGAGGA GAGGTTGTGA CCTTTCATTC CCTGGCAATA AACTGGTCTC TGGAGATCAC
    TGTAAAATTA TAGTGGATGA AAAATCTGGT CAGGTGTCAC TAGAAGATAC CAGCACCAAT
    GGAACAGTAA TTAACAAGCT GAAGGTTGTT AAGAAGCAGA CCTGCCCTTT GCAGACGGGG
    GATGTCATCT ACTTGGTGTA CAGGAAGAAT GAGCCAGAAC ACAATGTGGC ATACCTCTAT
    GAATCTTTAA ATGAGAAGCA GGACATCACA CAAGACTCCT TTGAAGCCAA TAAAGAGAAT
    GTGTTCCGTG TGACCAAAGA TACCTCAGGT GCAGGGCAAG GTGACGACCC CCAGGTCCTG
    CCGTCATCGC CCACCACTCA GGCGTGCTTT GAGGAACCAC AGCCATCAAC ATCCACATCA
    GACCTCTTCC CCACGGCTTC TACCTCTTCC ATGGAGCCCA CCTCTGCAGG GCAAGCACCT
    TCCTCCAGTT CTGGTGGGGA CCCTGACCTT ACCCTGCAGT TCTTGGTTGC AGACCAGTGT
    AGAGACACCC ACACCACCCT TGGGAATGTT AGATCCGAGG CTGTGAAGCC TGACAAGATG
    GAGGAGACAC TCACATGCAT CATCTGCCAG GACCTGCTGC ATGATTGTGT GAGCCTGCAG
    CCCTGCATGC ACACCTTCTG TGCAGCCTGC TACTCAGGCT GGATGGAGCG TTCTACCTGG
    TGTCCCACCT GCCGCTGTCC AGTCGAGAGG ATCTGTAAAA ACCACATCCT GAACAACCTG
    GTGGAGGCCT ACCTTCTCCA GCACCCAGAC AAGAGTCGCA GTGAGGAAGA TGTGCGGAGC
    ATGGCTGCCA GGAACAAGAT CACTCAAGAC ATGTTGCAAC CCAAGGTCAG AAGGTCTTTC
    TCTGATGAGG AGGGCAGTTC GGAAGATCTG CTAGAGCTGT CAGATGTGGA CAGTGAATCA
    TCGGATGTTA GCCAGCCGTA CATTGTATGC AGGCAGTGCC CTGAGTACCG GAGGCAGGGT
    GGGCAGGCCC TCCCCTGCCC AGGGCCTAAC AGTGAGCCAG GGGTACCACA GGCTGCTGGG
    GATGCGCCGT CAACCTCCAC CAGTGTCAAG ACAGCCCAGG ATTATGTGTG CGCCCTGCAA
    GGAAGCCATG CCATATGCAC CTGCTGCTTC CAGCCCATGC CTGACCGGAG GGCAGAACGT
    GAGCAGGACC CCCGCATCGC CCCCCAGCAG TGTGCCGTTT GCCTACAGCC TTTCTGCCAC
    CTGTACTGGG GCTGCGCCCG GGCGGGCTGC CTTGGCTGCC TGGCCCCATT CTGTGAGCTA
    GACCTGGGCG ACAGGTGTCT GGACGGGGTT CTGAGCAACA ACCACTACGA GTCAGACGTC
    CTGAAGAATT ATCTGGCAAC CAGGGGTTTG ACATGGAAAA ATATGTTGAC AGAAAGTCTG
    GTGGCTTTGC AGAGGGGAGC GTTTTTACTA TCTGATTACA GAATCACGGG GAACACCGTG
    CTGTGTTACT GCTGTGGCCT GCGGAGCTTC CGAGAGCTGA CCTACCAGTA TCGGCAGAAC
    ATCCCTGCTT CTGAGTTGCC AGTGGCTGTA ACATCCCGTC CTGATTGCTA TTGGGGGCGC
    AACTGCCGCA CTCAGGTGAA GGCCCACCAC GCAATGAAAT TCAATCATAT CTGTGAACAG
    ACAAGGTTCA AGAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005636122.1
    CDS178..1995
    Translation

    Target ORF information:

    RefSeq Version XM_005636065.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase (CHFR), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005636065.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGGAGCAGC GCCGGGAAGG CGAGCAGCCG CCGCAGCAGC AGCCGTGGGG GAGGCTCCTC 
    CGCTTGGGCG CCGGGGACGG CGAGCCGCAC GTCCTCCTGA GGAACCGGGA GTGGACCATC
    GGGCGGAGGA GAGGTTGTGA CCTTTCATTC CCTGGCAATA AACTGGTCTC TGGAGATCAC
    TGTAAAATTA TAGTGGATGA AAAATCTGGT CAGGTGTCAC TAGAAGATAC CAGCACCAAT
    GGAACAGTAA TTAACAAGCT GAAGGTTGTT AAGAAGCAGA CCTGCCCTTT GCAGACGGGG
    GATGTCATCT ACTTGGTGTA CAGGAAGAAT GAGCCAGAAC ACAATGTGGC ATACCTCTAT
    GAATCTTTAA ATGAGAAGCA GGACATCACA CAAGACTCCT TTGAAGCCAA TAAAGAGAAT
    GTGTTCCGTG TGACCAAAGA TACCTCAGGT GCAGGGCAAG GTGACGACCC CCAGGTCCTG
    CCGTCATCGC CCACCACTCA GGCGTGCTTT GAGGAACCAC AGCCATCAAC ATCCACATCA
    GACCTCTTCC CCACGGCTTC TACCTCTTCC ATGGAGCCCA CCTCTGCAGG GCAAGCACCT
    TCCTCCAGTT CTGGTGGGGA CCCTGACCTT ACCCTGCAGT TCTTGGTTGC AGACCAGTGT
    AGAGACACCC ACACCACCCT TGGGAATGTT AGATCCGAGG CTGTGAAGCC TGACAAGATG
    GAGGAGACAC TCACATGCAT CATCTGCCAG GACCTGCTGC ATGATTGTGT GAGCCTGCAG
    CCCTGCATGC ACACCTTCTG TGCAGCCTGC TACTCAGGCT GGATGGAGCG TTCTACCTGG
    TGTCCCACCT GCCGCTGTCC AGTCGAGAGG ATCTGTAAAA ACCACATCCT GAACAACCTG
    GTGGAGGCCT ACCTTCTCCA GCACCCAGAC AAGAGTCGCA GTGAGGAAGA TGTGCGGAGC
    ATGGCTGCCA GGAACAAGAT CACTCAAGAC ATGTTGCAAC CCAAGGTCAG AAGGTCTTTC
    TCTGATGAGG AGGGCAGTTC GGAAGATCTG CTAGAGCTGT CAGATGTGGA CAGTGAATCA
    TCGGATGTTA GCCAGCCGTA CATTGTATGC AGGCAGTGCC CTGAGTACCG GAGGCAGGGT
    GGGCAGGCCC TCCCCTGCCC AGGGCCTAAC AGTGAGCCAG GGGTACCACA GGCTGCTGGG
    GATGCGCCGT CAACCTCCAC CAGTGTCAAG ACAGCCCAGG ATTATGTGTG CGCCCTGCAA
    GGAAGCCATG CCATATGCAC CTGCTGCTTC CAGCCCATGC CTGACCGGAG GGCAGAACGT
    GAGCAGGACC CCCGCATCGC CCCCCAGCAG TGTGCCGTTT GCCTACAGCC TTTCTGCCAC
    CTGTACTGGG GCTGCGCCCG GGCGGGCTGC CTTGGCTGCC TGGCCCCATT CTGTGAGCTA
    GACCTGGGCG ACAGGTGTCT GGACGGGGTT CTGAGCAACA ACCACTACGA GTCAGACGTC
    CTGAAGAATT ATCTGGCAAC CAGGGGTTTG ACATGGAAAA ATATGTTGAC AGAAAGTCTG
    GTGGCTTTGC AGAGGGGAGC GTTTTTACTA TCTGATTACA GAATCACGGG GAACACCGTG
    CTGTGTTACT GCTGTGGCCT GCGGAGCTTC CGAGAGCTGA CCTACCAGTA TCGGCAGAAC
    ATCCCTGCTT CTGAGTTGCC AGTGGCTGTA ACATCCCGTC CTGATTGCTA TTGGGGGCGC
    AACTGCCGCA CTCAGGTGAA GGCCCACCAC GCAATGAAAT TCAATCATAT CTGTGAACAG
    ACAAGGTTCA AGAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe26049
    Clone ID Related Accession (Same CDS sequence) XM_005636065.1 , XM_022410416.1
    Accession Version XM_022410416.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1818bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product E3 ubiquitin-protein ligase CHFR isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006608.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGGAGCAGC GCCGGGAAGG CGAGCAGCCG CCGCAGCAGC AGCCGTGGGG GAGGCTCCTC 
    CGCTTGGGCG CCGGGGACGG CGAGCCGCAC GTCCTCCTGA GGAACCGGGA GTGGACCATC
    GGGCGGAGGA GAGGTTGTGA CCTTTCATTC CCTGGCAATA AACTGGTCTC TGGAGATCAC
    TGTAAAATTA TAGTGGATGA AAAATCTGGT CAGGTGTCAC TAGAAGATAC CAGCACCAAT
    GGAACAGTAA TTAACAAGCT GAAGGTTGTT AAGAAGCAGA CCTGCCCTTT GCAGACGGGG
    GATGTCATCT ACTTGGTGTA CAGGAAGAAT GAGCCAGAAC ACAATGTGGC ATACCTCTAT
    GAATCTTTAA ATGAGAAGCA GGACATCACA CAAGACTCCT TTGAAGCCAA TAAAGAGAAT
    GTGTTCCGTG TGACCAAAGA TACCTCAGGT GCAGGGCAAG GTGACGACCC CCAGGTCCTG
    CCGTCATCGC CCACCACTCA GGCGTGCTTT GAGGAACCAC AGCCATCAAC ATCCACATCA
    GACCTCTTCC CCACGGCTTC TACCTCTTCC ATGGAGCCCA CCTCTGCAGG GCAAGCACCT
    TCCTCCAGTT CTGGTGGGGA CCCTGACCTT ACCCTGCAGT TCTTGGTTGC AGACCAGTGT
    AGAGACACCC ACACCACCCT TGGGAATGTT AGATCCGAGG CTGTGAAGCC TGACAAGATG
    GAGGAGACAC TCACATGCAT CATCTGCCAG GACCTGCTGC ATGATTGTGT GAGCCTGCAG
    CCCTGCATGC ACACCTTCTG TGCAGCCTGC TACTCAGGCT GGATGGAGCG TTCTACCTGG
    TGTCCCACCT GCCGCTGTCC AGTCGAGAGG ATCTGTAAAA ACCACATCCT GAACAACCTG
    GTGGAGGCCT ACCTTCTCCA GCACCCAGAC AAGAGTCGCA GTGAGGAAGA TGTGCGGAGC
    ATGGCTGCCA GGAACAAGAT CACTCAAGAC ATGTTGCAAC CCAAGGTCAG AAGGTCTTTC
    TCTGATGAGG AGGGCAGTTC GGAAGATCTG CTAGAGCTGT CAGATGTGGA CAGTGAATCA
    TCGGATGTTA GCCAGCCGTA CATTGTATGC AGGCAGTGCC CTGAGTACCG GAGGCAGGGT
    GGGCAGGCCC TCCCCTGCCC AGGGCCTAAC AGTGAGCCAG GGGTACCACA GGCTGCTGGG
    GATGCGCCGT CAACCTCCAC CAGTGTCAAG ACAGCCCAGG ATTATGTGTG CGCCCTGCAA
    GGAAGCCATG CCATATGCAC CTGCTGCTTC CAGCCCATGC CTGACCGGAG GGCAGAACGT
    GAGCAGGACC CCCGCATCGC CCCCCAGCAG TGTGCCGTTT GCCTACAGCC TTTCTGCCAC
    CTGTACTGGG GCTGCGCCCG GGCGGGCTGC CTTGGCTGCC TGGCCCCATT CTGTGAGCTA
    GACCTGGGCG ACAGGTGTCT GGACGGGGTT CTGAGCAACA ACCACTACGA GTCAGACGTC
    CTGAAGAATT ATCTGGCAAC CAGGGGTTTG ACATGGAAAA ATATGTTGAC AGAAAGTCTG
    GTGGCTTTGC AGAGGGGAGC GTTTTTACTA TCTGATTACA GAATCACGGG GAACACCGTG
    CTGTGTTACT GCTGTGGCCT GCGGAGCTTC CGAGAGCTGA CCTACCAGTA TCGGCAGAAC
    ATCCCTGCTT CTGAGTTGCC AGTGGCTGTA ACATCCCGTC CTGATTGCTA TTGGGGGCGC
    AACTGCCGCA CTCAGGTGAA GGCCCACCAC GCAATGAAAT TCAATCATAT CTGTGAACAG
    ACAAGGTTCA AGAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022266124.1
    CDS12..1829
    Translation

    Target ORF information:

    RefSeq Version XM_022410416.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris checkpoint with forkhead and ring finger domains (CHFR), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022410416.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGGAGCAGC GCCGGGAAGG CGAGCAGCCG CCGCAGCAGC AGCCGTGGGG GAGGCTCCTC 
    CGCTTGGGCG CCGGGGACGG CGAGCCGCAC GTCCTCCTGA GGAACCGGGA GTGGACCATC
    GGGCGGAGGA GAGGTTGTGA CCTTTCATTC CCTGGCAATA AACTGGTCTC TGGAGATCAC
    TGTAAAATTA TAGTGGATGA AAAATCTGGT CAGGTGTCAC TAGAAGATAC CAGCACCAAT
    GGAACAGTAA TTAACAAGCT GAAGGTTGTT AAGAAGCAGA CCTGCCCTTT GCAGACGGGG
    GATGTCATCT ACTTGGTGTA CAGGAAGAAT GAGCCAGAAC ACAATGTGGC ATACCTCTAT
    GAATCTTTAA ATGAGAAGCA GGACATCACA CAAGACTCCT TTGAAGCCAA TAAAGAGAAT
    GTGTTCCGTG TGACCAAAGA TACCTCAGGT GCAGGGCAAG GTGACGACCC CCAGGTCCTG
    CCGTCATCGC CCACCACTCA GGCGTGCTTT GAGGAACCAC AGCCATCAAC ATCCACATCA
    GACCTCTTCC CCACGGCTTC TACCTCTTCC ATGGAGCCCA CCTCTGCAGG GCAAGCACCT
    TCCTCCAGTT CTGGTGGGGA CCCTGACCTT ACCCTGCAGT TCTTGGTTGC AGACCAGTGT
    AGAGACACCC ACACCACCCT TGGGAATGTT AGATCCGAGG CTGTGAAGCC TGACAAGATG
    GAGGAGACAC TCACATGCAT CATCTGCCAG GACCTGCTGC ATGATTGTGT GAGCCTGCAG
    CCCTGCATGC ACACCTTCTG TGCAGCCTGC TACTCAGGCT GGATGGAGCG TTCTACCTGG
    TGTCCCACCT GCCGCTGTCC AGTCGAGAGG ATCTGTAAAA ACCACATCCT GAACAACCTG
    GTGGAGGCCT ACCTTCTCCA GCACCCAGAC AAGAGTCGCA GTGAGGAAGA TGTGCGGAGC
    ATGGCTGCCA GGAACAAGAT CACTCAAGAC ATGTTGCAAC CCAAGGTCAG AAGGTCTTTC
    TCTGATGAGG AGGGCAGTTC GGAAGATCTG CTAGAGCTGT CAGATGTGGA CAGTGAATCA
    TCGGATGTTA GCCAGCCGTA CATTGTATGC AGGCAGTGCC CTGAGTACCG GAGGCAGGGT
    GGGCAGGCCC TCCCCTGCCC AGGGCCTAAC AGTGAGCCAG GGGTACCACA GGCTGCTGGG
    GATGCGCCGT CAACCTCCAC CAGTGTCAAG ACAGCCCAGG ATTATGTGTG CGCCCTGCAA
    GGAAGCCATG CCATATGCAC CTGCTGCTTC CAGCCCATGC CTGACCGGAG GGCAGAACGT
    GAGCAGGACC CCCGCATCGC CCCCCAGCAG TGTGCCGTTT GCCTACAGCC TTTCTGCCAC
    CTGTACTGGG GCTGCGCCCG GGCGGGCTGC CTTGGCTGCC TGGCCCCATT CTGTGAGCTA
    GACCTGGGCG ACAGGTGTCT GGACGGGGTT CTGAGCAACA ACCACTACGA GTCAGACGTC
    CTGAAGAATT ATCTGGCAAC CAGGGGTTTG ACATGGAAAA ATATGTTGAC AGAAAGTCTG
    GTGGCTTTGC AGAGGGGAGC GTTTTTACTA TCTGATTACA GAATCACGGG GAACACCGTG
    CTGTGTTACT GCTGTGGCCT GCGGAGCTTC CGAGAGCTGA CCTACCAGTA TCGGCAGAAC
    ATCCCTGCTT CTGAGTTGCC AGTGGCTGTA ACATCCCGTC CTGATTGCTA TTGGGGGCGC
    AACTGCCGCA CTCAGGTGAA GGCCCACCAC GCAATGAAAT TCAATCATAT CTGTGAACAG
    ACAAGGTTCA AGAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe282435
    Clone ID Related Accession (Same CDS sequence) XM_022410414.1 , XM_022410415.1
    Accession Version XM_022410414.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2019bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product E3 ubiquitin-protein ligase CHFR isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006608.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGAACAAG TCTGTGAAAC AAATGCAAGT GGAAAAATAT TTCAGTTTGG CATGAACCTT 
    CCACCTTTAA AGCAAATGCC AAATAAGCAT GTCTCAAATG GAAAACATCT GAAATACTCA
    TTAGATTTAT TTACTCAGAC TGGAAATTGT GGAAGAAAGA AAGCTGGTTG TGACCTTTCA
    TTCCCTGGCA ATAAACTGGT CTCTGGAGAT CACTGTAAAA TTATAGTGGA TGAAAAATCT
    GGTCAGGTGT CACTAGAAGA TACCAGCACC AATGGAACAG TAATTAACAA GCTGAAGGTT
    GTTAAGAAGC AGACCTGCCC TTTGCAGACG GGGGATGTCA TCTACTTGGT GTACAGGAAG
    AATGAGCCAG AACACAATGT GGCATACCTC TATGAATCTT TAAATGAGAA GCAGGACATC
    ACACAAGACT CCTTTGAAGC CAATAAAGAG AATGTGTTCC GTGTGACCAA AGATACCTCA
    GGTGCAGGGC AAGGTGACGA CCCCCAGGTC CTGCCGTCAT CGCCCACCAC TCAGGCGTGC
    TTTGAGGAAC CACAGCCATC AACATCCACA TCAGACCTCT TCCCCACGGC TTCTACCTCT
    TCCATGGAGC CCACCTCTGC AGGGCAAGCA CCTTCCTCCA GTTCTGCTTC CAGAGATGCA
    TACATCTCCC CAAAAGAGTG TGGTCCATCT GTGGCAAGTG ATGAAATCCC CATCTTTCCT
    TCAGCTCTGC CAGACAGAGA GAGAGCATCC TTTTCTCTGT CGGAACCCCA GGATCAGGAG
    GATTTGGAGC CCATCAGGAA GAAAATGAAA GGAGGTGGGG ACCCTGACCT TACCCTGCAG
    TTCTTGGTTG CAGACCAGTG TAGAGACACC CACACCACCC TTGGGAATGT TAGATCCGAG
    GCTGTGAAGC CTGACAAGAT GGAGGAGACA CTCACATGCA TCATCTGCCA GGACCTGCTG
    CATGATTGTG TGAGCCTGCA GCCCTGCATG CACACCTTCT GTGCAGCCTG CTACTCAGGC
    TGGATGGAGC GTTCTACCTG GTGTCCCACC TGCCGCTGTC CAGTCGAGAG GATCTGTAAA
    AACCACATCC TGAACAACCT GGTGGAGGCC TACCTTCTCC AGCACCCAGA CAAGAGTCGC
    AGTGAGGAAG ATGTGCGGAG CATGGCTGCC AGGAACAAGA TCACTCAAGA CATGTTGCAA
    CCCAAGGTCA GAAGGTCTTT CTCTGATGAG GAGGGCAGTT CGGAAGATCT GCTAGAGCTG
    TCAGATGTGG ACAGTGAATC ATCGGATGTT AGCCAGCCGT ACATTGTATG CAGGCAGTGC
    CCTGAGTACC GGAGGCAGGG TGGGCAGGCC CTCCCCTGCC CAGGGCCTAA CAGTGAGCCA
    GGGGTACCAC AGGCTGCTGG GGATGCGCCG TCAACCTCCA CCAGTGTCAA GACAGCCCAG
    GATTATGTGT GCGCCCTGCA AGGAAGCCAT GCCATATGCA CCTGCTGCTT CCAGCCCATG
    CCTGACCGGA GGGCAGAACG TGAGCAGGAC CCCCGCATCG CCCCCCAGCA GTGTGCCGTT
    TGCCTACAGC CTTTCTGCCA CCTGTACTGG GGCTGCGCCC GGGCGGGCTG CCTTGGCTGC
    CTGGCCCCAT TCTGTGAGCT AGACCTGGGC GACAGGTGTC TGGACGGGGT TCTGAGCAAC
    AACCACTACG AGTCAGACGT CCTGAAGAAT TATCTGGCAA CCAGGGGTTT GACATGGAAA
    AATATGTTGA CAGAAAGTCT GGTGGCTTTG CAGAGGGGAG CGTTTTTACT ATCTGATTAC
    AGAATCACGG GGAACACCGT GCTGTGTTAC TGCTGTGGCC TGCGGAGCTT CCGAGAGCTG
    ACCTACCAGT ATCGGCAGAA CATCCCTGCT TCTGAGTTGC CAGTGGCTGT AACATCCCGT
    CCTGATTGCT ATTGGGGGCG CAACTGCCGC ACTCAGGTGA AGGCCCACCA CGCAATGAAA
    TTCAATCATA TCTGTGAACA GACAAGGTTC AAGAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022266122.1
    CDS295..2313
    Translation

    Target ORF information:

    RefSeq Version XM_022410414.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris checkpoint with forkhead and ring finger domains (CHFR), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022410414.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGAACAAG TCTGTGAAAC AAATGCAAGT GGAAAAATAT TTCAGTTTGG CATGAACCTT 
    CCACCTTTAA AGCAAATGCC AAATAAGCAT GTCTCAAATG GAAAACATCT GAAATACTCA
    TTAGATTTAT TTACTCAGAC TGGAAATTGT GGAAGAAAGA AAGCTGGTTG TGACCTTTCA
    TTCCCTGGCA ATAAACTGGT CTCTGGAGAT CACTGTAAAA TTATAGTGGA TGAAAAATCT
    GGTCAGGTGT CACTAGAAGA TACCAGCACC AATGGAACAG TAATTAACAA GCTGAAGGTT
    GTTAAGAAGC AGACCTGCCC TTTGCAGACG GGGGATGTCA TCTACTTGGT GTACAGGAAG
    AATGAGCCAG AACACAATGT GGCATACCTC TATGAATCTT TAAATGAGAA GCAGGACATC
    ACACAAGACT CCTTTGAAGC CAATAAAGAG AATGTGTTCC GTGTGACCAA AGATACCTCA
    GGTGCAGGGC AAGGTGACGA CCCCCAGGTC CTGCCGTCAT CGCCCACCAC TCAGGCGTGC
    TTTGAGGAAC CACAGCCATC AACATCCACA TCAGACCTCT TCCCCACGGC TTCTACCTCT
    TCCATGGAGC CCACCTCTGC AGGGCAAGCA CCTTCCTCCA GTTCTGCTTC CAGAGATGCA
    TACATCTCCC CAAAAGAGTG TGGTCCATCT GTGGCAAGTG ATGAAATCCC CATCTTTCCT
    TCAGCTCTGC CAGACAGAGA GAGAGCATCC TTTTCTCTGT CGGAACCCCA GGATCAGGAG
    GATTTGGAGC CCATCAGGAA GAAAATGAAA GGAGGTGGGG ACCCTGACCT TACCCTGCAG
    TTCTTGGTTG CAGACCAGTG TAGAGACACC CACACCACCC TTGGGAATGT TAGATCCGAG
    GCTGTGAAGC CTGACAAGAT GGAGGAGACA CTCACATGCA TCATCTGCCA GGACCTGCTG
    CATGATTGTG TGAGCCTGCA GCCCTGCATG CACACCTTCT GTGCAGCCTG CTACTCAGGC
    TGGATGGAGC GTTCTACCTG GTGTCCCACC TGCCGCTGTC CAGTCGAGAG GATCTGTAAA
    AACCACATCC TGAACAACCT GGTGGAGGCC TACCTTCTCC AGCACCCAGA CAAGAGTCGC
    AGTGAGGAAG ATGTGCGGAG CATGGCTGCC AGGAACAAGA TCACTCAAGA CATGTTGCAA
    CCCAAGGTCA GAAGGTCTTT CTCTGATGAG GAGGGCAGTT CGGAAGATCT GCTAGAGCTG
    TCAGATGTGG ACAGTGAATC ATCGGATGTT AGCCAGCCGT ACATTGTATG CAGGCAGTGC
    CCTGAGTACC GGAGGCAGGG TGGGCAGGCC CTCCCCTGCC CAGGGCCTAA CAGTGAGCCA
    GGGGTACCAC AGGCTGCTGG GGATGCGCCG TCAACCTCCA CCAGTGTCAA GACAGCCCAG
    GATTATGTGT GCGCCCTGCA AGGAAGCCAT GCCATATGCA CCTGCTGCTT CCAGCCCATG
    CCTGACCGGA GGGCAGAACG TGAGCAGGAC CCCCGCATCG CCCCCCAGCA GTGTGCCGTT
    TGCCTACAGC CTTTCTGCCA CCTGTACTGG GGCTGCGCCC GGGCGGGCTG CCTTGGCTGC
    CTGGCCCCAT TCTGTGAGCT AGACCTGGGC GACAGGTGTC TGGACGGGGT TCTGAGCAAC
    AACCACTACG AGTCAGACGT CCTGAAGAAT TATCTGGCAA CCAGGGGTTT GACATGGAAA
    AATATGTTGA CAGAAAGTCT GGTGGCTTTG CAGAGGGGAG CGTTTTTACT ATCTGATTAC
    AGAATCACGG GGAACACCGT GCTGTGTTAC TGCTGTGGCC TGCGGAGCTT CCGAGAGCTG
    ACCTACCAGT ATCGGCAGAA CATCCCTGCT TCTGAGTTGC CAGTGGCTGT AACATCCCGT
    CCTGATTGCT ATTGGGGGCG CAACTGCCGC ACTCAGGTGA AGGCCCACCA CGCAATGAAA
    TTCAATCATA TCTGTGAACA GACAAGGTTC AAGAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe282435
    Clone ID Related Accession (Same CDS sequence) XM_022410414.1 , XM_022410415.1
    Accession Version XM_022410415.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2019bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product E3 ubiquitin-protein ligase CHFR isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006608.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGAACAAG TCTGTGAAAC AAATGCAAGT GGAAAAATAT TTCAGTTTGG CATGAACCTT 
    CCACCTTTAA AGCAAATGCC AAATAAGCAT GTCTCAAATG GAAAACATCT GAAATACTCA
    TTAGATTTAT TTACTCAGAC TGGAAATTGT GGAAGAAAGA AAGCTGGTTG TGACCTTTCA
    TTCCCTGGCA ATAAACTGGT CTCTGGAGAT CACTGTAAAA TTATAGTGGA TGAAAAATCT
    GGTCAGGTGT CACTAGAAGA TACCAGCACC AATGGAACAG TAATTAACAA GCTGAAGGTT
    GTTAAGAAGC AGACCTGCCC TTTGCAGACG GGGGATGTCA TCTACTTGGT GTACAGGAAG
    AATGAGCCAG AACACAATGT GGCATACCTC TATGAATCTT TAAATGAGAA GCAGGACATC
    ACACAAGACT CCTTTGAAGC CAATAAAGAG AATGTGTTCC GTGTGACCAA AGATACCTCA
    GGTGCAGGGC AAGGTGACGA CCCCCAGGTC CTGCCGTCAT CGCCCACCAC TCAGGCGTGC
    TTTGAGGAAC CACAGCCATC AACATCCACA TCAGACCTCT TCCCCACGGC TTCTACCTCT
    TCCATGGAGC CCACCTCTGC AGGGCAAGCA CCTTCCTCCA GTTCTGCTTC CAGAGATGCA
    TACATCTCCC CAAAAGAGTG TGGTCCATCT GTGGCAAGTG ATGAAATCCC CATCTTTCCT
    TCAGCTCTGC CAGACAGAGA GAGAGCATCC TTTTCTCTGT CGGAACCCCA GGATCAGGAG
    GATTTGGAGC CCATCAGGAA GAAAATGAAA GGAGGTGGGG ACCCTGACCT TACCCTGCAG
    TTCTTGGTTG CAGACCAGTG TAGAGACACC CACACCACCC TTGGGAATGT TAGATCCGAG
    GCTGTGAAGC CTGACAAGAT GGAGGAGACA CTCACATGCA TCATCTGCCA GGACCTGCTG
    CATGATTGTG TGAGCCTGCA GCCCTGCATG CACACCTTCT GTGCAGCCTG CTACTCAGGC
    TGGATGGAGC GTTCTACCTG GTGTCCCACC TGCCGCTGTC CAGTCGAGAG GATCTGTAAA
    AACCACATCC TGAACAACCT GGTGGAGGCC TACCTTCTCC AGCACCCAGA CAAGAGTCGC
    AGTGAGGAAG ATGTGCGGAG CATGGCTGCC AGGAACAAGA TCACTCAAGA CATGTTGCAA
    CCCAAGGTCA GAAGGTCTTT CTCTGATGAG GAGGGCAGTT CGGAAGATCT GCTAGAGCTG
    TCAGATGTGG ACAGTGAATC ATCGGATGTT AGCCAGCCGT ACATTGTATG CAGGCAGTGC
    CCTGAGTACC GGAGGCAGGG TGGGCAGGCC CTCCCCTGCC CAGGGCCTAA CAGTGAGCCA
    GGGGTACCAC AGGCTGCTGG GGATGCGCCG TCAACCTCCA CCAGTGTCAA GACAGCCCAG
    GATTATGTGT GCGCCCTGCA AGGAAGCCAT GCCATATGCA CCTGCTGCTT CCAGCCCATG
    CCTGACCGGA GGGCAGAACG TGAGCAGGAC CCCCGCATCG CCCCCCAGCA GTGTGCCGTT
    TGCCTACAGC CTTTCTGCCA CCTGTACTGG GGCTGCGCCC GGGCGGGCTG CCTTGGCTGC
    CTGGCCCCAT TCTGTGAGCT AGACCTGGGC GACAGGTGTC TGGACGGGGT TCTGAGCAAC
    AACCACTACG AGTCAGACGT CCTGAAGAAT TATCTGGCAA CCAGGGGTTT GACATGGAAA
    AATATGTTGA CAGAAAGTCT GGTGGCTTTG CAGAGGGGAG CGTTTTTACT ATCTGATTAC
    AGAATCACGG GGAACACCGT GCTGTGTTAC TGCTGTGGCC TGCGGAGCTT CCGAGAGCTG
    ACCTACCAGT ATCGGCAGAA CATCCCTGCT TCTGAGTTGC CAGTGGCTGT AACATCCCGT
    CCTGATTGCT ATTGGGGGCG CAACTGCCGC ACTCAGGTGA AGGCCCACCA CGCAATGAAA
    TTCAATCATA TCTGTGAACA GACAAGGTTC AAGAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022266123.1
    CDS255..2273
    Translation

    Target ORF information:

    RefSeq Version XM_022410415.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris checkpoint with forkhead and ring finger domains (CHFR), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022410415.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGAACAAG TCTGTGAAAC AAATGCAAGT GGAAAAATAT TTCAGTTTGG CATGAACCTT 
    CCACCTTTAA AGCAAATGCC AAATAAGCAT GTCTCAAATG GAAAACATCT GAAATACTCA
    TTAGATTTAT TTACTCAGAC TGGAAATTGT GGAAGAAAGA AAGCTGGTTG TGACCTTTCA
    TTCCCTGGCA ATAAACTGGT CTCTGGAGAT CACTGTAAAA TTATAGTGGA TGAAAAATCT
    GGTCAGGTGT CACTAGAAGA TACCAGCACC AATGGAACAG TAATTAACAA GCTGAAGGTT
    GTTAAGAAGC AGACCTGCCC TTTGCAGACG GGGGATGTCA TCTACTTGGT GTACAGGAAG
    AATGAGCCAG AACACAATGT GGCATACCTC TATGAATCTT TAAATGAGAA GCAGGACATC
    ACACAAGACT CCTTTGAAGC CAATAAAGAG AATGTGTTCC GTGTGACCAA AGATACCTCA
    GGTGCAGGGC AAGGTGACGA CCCCCAGGTC CTGCCGTCAT CGCCCACCAC TCAGGCGTGC
    TTTGAGGAAC CACAGCCATC AACATCCACA TCAGACCTCT TCCCCACGGC TTCTACCTCT
    TCCATGGAGC CCACCTCTGC AGGGCAAGCA CCTTCCTCCA GTTCTGCTTC CAGAGATGCA
    TACATCTCCC CAAAAGAGTG TGGTCCATCT GTGGCAAGTG ATGAAATCCC CATCTTTCCT
    TCAGCTCTGC CAGACAGAGA GAGAGCATCC TTTTCTCTGT CGGAACCCCA GGATCAGGAG
    GATTTGGAGC CCATCAGGAA GAAAATGAAA GGAGGTGGGG ACCCTGACCT TACCCTGCAG
    TTCTTGGTTG CAGACCAGTG TAGAGACACC CACACCACCC TTGGGAATGT TAGATCCGAG
    GCTGTGAAGC CTGACAAGAT GGAGGAGACA CTCACATGCA TCATCTGCCA GGACCTGCTG
    CATGATTGTG TGAGCCTGCA GCCCTGCATG CACACCTTCT GTGCAGCCTG CTACTCAGGC
    TGGATGGAGC GTTCTACCTG GTGTCCCACC TGCCGCTGTC CAGTCGAGAG GATCTGTAAA
    AACCACATCC TGAACAACCT GGTGGAGGCC TACCTTCTCC AGCACCCAGA CAAGAGTCGC
    AGTGAGGAAG ATGTGCGGAG CATGGCTGCC AGGAACAAGA TCACTCAAGA CATGTTGCAA
    CCCAAGGTCA GAAGGTCTTT CTCTGATGAG GAGGGCAGTT CGGAAGATCT GCTAGAGCTG
    TCAGATGTGG ACAGTGAATC ATCGGATGTT AGCCAGCCGT ACATTGTATG CAGGCAGTGC
    CCTGAGTACC GGAGGCAGGG TGGGCAGGCC CTCCCCTGCC CAGGGCCTAA CAGTGAGCCA
    GGGGTACCAC AGGCTGCTGG GGATGCGCCG TCAACCTCCA CCAGTGTCAA GACAGCCCAG
    GATTATGTGT GCGCCCTGCA AGGAAGCCAT GCCATATGCA CCTGCTGCTT CCAGCCCATG
    CCTGACCGGA GGGCAGAACG TGAGCAGGAC CCCCGCATCG CCCCCCAGCA GTGTGCCGTT
    TGCCTACAGC CTTTCTGCCA CCTGTACTGG GGCTGCGCCC GGGCGGGCTG CCTTGGCTGC
    CTGGCCCCAT TCTGTGAGCT AGACCTGGGC GACAGGTGTC TGGACGGGGT TCTGAGCAAC
    AACCACTACG AGTCAGACGT CCTGAAGAAT TATCTGGCAA CCAGGGGTTT GACATGGAAA
    AATATGTTGA CAGAAAGTCT GGTGGCTTTG CAGAGGGGAG CGTTTTTACT ATCTGATTAC
    AGAATCACGG GGAACACCGT GCTGTGTTAC TGCTGTGGCC TGCGGAGCTT CCGAGAGCTG
    ACCTACCAGT ATCGGCAGAA CATCCCTGCT TCTGAGTTGC CAGTGGCTGT AACATCCCGT
    CCTGATTGCT ATTGGGGGCG CAACTGCCGC ACTCAGGTGA AGGCCCACCA CGCAATGAAA
    TTCAATCATA TCTGTGAACA GACAAGGTTC AAGAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.