chfr cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	chfr
Entrez Gene ID	564271
Full Name	checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase
Synonyms	fc43f10,si:dkey-69h6.7,wu:fc43f10
General protein information	Preferred Names checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase Names E3 ubiquitin-protein ligase CHFR Checkpoint with forkhead and RING finger domains protein RING-type E3 ubiquitin transferase CHFR
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017356139.1	XP_017211628.1	E3 ubiquitin-protein ligase CHFR isoform X2
XM_017356138.1	XP_017211627.1	E3 ubiquitin-protein ligase CHFR isoform X1
NM_001100015.1	NP_001093485.1	E3 ubiquitin-protein ligase CHFR
XM_017356139.2	XP_017211628.1	E3 ubiquitin-protein ligase CHFR isoform X2
XM_021476266.1	XP_021331941.1	E3 ubiquitin-protein ligase CHFR isoform X2
XM_017356138.2	XP_017211627.1	E3 ubiquitin-protein ligase CHFR isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Chfr	NP_001009258.1
Xenopus tropicalis (tropical clawed frog)	chfr	NP_989378.1
	CHFR	XP_001083796.2
Oryza sativa (rice)	Os11g0175500	NP_001065879.2
Homo sapiens (human)	CHFR	NP_001154816.1
Pan troglodytes (chimpanzee)	CHFR	XP_003314094.2
Bos taurus (cattle)	CHFR	NP_001192403.1
Danio rerio (zebrafish)	chfr	NP_001093485.1
Canis lupus familiaris (dog)	CHFR	XP_534631.3
Arabidopsis thaliana (thale cress)	AT1G47570	NP_001077687.1
Mus musculus (house mouse)	Chfr	NP_766305.2
Gallus gallus (chicken)	CHFR	XP_004945549.1

Gene Ontology
Bibliography

Related articles in PubMed

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following chfr gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the chfr cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa00246	NM_001100015.1 Latest version!	Danio rerio checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase (chfr), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa00246	XM_017356138.2 Latest version!	Danio rerio checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase (chfr), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa10198	XM_017356139.2 Latest version!	Danio rerio checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase (chfr), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa10198	XM_021476266.1 Latest version!	Danio rerio checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase (chfr), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
View Old Accession Versions >>
ODa10198	XM_017356139.1	Danio rerio checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase (chfr), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa00246	XM_017356138.1	Danio rerio checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase (chfr), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

chfr ( NM_001100015.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001093485.1 Danio rerio checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase (chfr), mRNA.

Price & Availability↑

CloneID	ODa00246
Clone ID Related Accession (Same CDS sequence)	XM_017356138.1 , NM_001100015.1 , XM_017356138.2
Accession Version	NM_001100015.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1914bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-21
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase CHFR
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CT573109.10. On Jul 26, 2007 this sequence version replaced XM_687615.2. ##Evidence-Data-START## Transcript exon combination :: GFIL01003891.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGACAAATC AGGACAGCGA TCAAGCCTGG GGGAAACTGG TGAAAGTGGA CGCATCTCCA 
GGTTCGGAGA TAGTGCTTAT AAATAGTGAA TGCACCGTTG GGAGAAAGAA AGATTGTGAC 
CTCTCATTCC CAGCTAATAA ACTGGTCTCA GGAAATCATT GCAAAATCAC TCATGATCAA 
AACTCAGGAA AGGTGTGGCT GGAAGACATG AGCACCAACG GAACTGTGAT CAACATGTCC 
AAAGTAGTGA AGAAACAGAC GCACTTACTG CAGAACGGTG ATGTCATTTA CTTTGTGTAC 
AGGAAGAACG AGCCAGAGCA AAACATTGCT TATGTTTATC AGTCCATCAC GCCACAGGAA 
AGTGCCTCAC ATGATGTTGA AGATGCAGGG AGAGAAGAAG ATTCTGATCT GACAGAAACG 
GAGAGTGAAC CAGCACCAGT AGAGCCTGTT ATCGTGAAGC CTTTACCTCA GTCTGGACAT 
GAGGATCCTC AGCCATCTAC CTCCAGCTCT TCCTTACACT TCTACAACAT GCCATTGTCT 
ACCTGCTCTG ATGTGTCTGC AAGAAAGAAT CCAGTCAGTT CTTCAGCTGT TTGTAAAGGA 
GACTCTACTT CATCAGGAAG TCCCGCTCAA ACCCGGCTGA AGTGGACCTG CTGGACTGAT 
GGAGAGCCGG AGGAGGAAAT GCAGAGGAAA AGAAGAAAAA CTGACAGAGA TGATCCAGGG 
TTTGGATCTG CTCACAGTGA TGCTTCTGCT GACATTCCCC TCAGAGGAGC AAGTGGAAAA 
GAAAAAACTG AAGGAGCCAC AACAGACAAG ATGGAGGAAT CCCTCACATG CATCATCTGT 
CAGGACCTGC TATATGACTG CATCAGTGTT CAGCCTTGCA TGCATACGTT TTGTGCGGCC 
TGTTATTCTG GCTGGATGGA GCGTTCCTCT TTCTGTCCCA CCTGTCGATG TCCTGTGGAG 
AGGATCCGCA AAAACCACAT CCTCAACAAC CTGGTGGAGG CTTACCTTCT CCAGCACCCG 
GAGAAGTGTC GGACTGAAGA TGATTTGCGC AGTATGGATG CCAGAAACAA GATCACACAG 
GACATGCTGC AGCCGAAGGT CGAGCGATCG TTCTCAGACG AGGAGGCCAG TTCAGACTAT 
CTGTTTGAAC TCTCAGACAA TGACAGCGAC ATTTCTGACA TGAGCCAACC CTACATGATG 
TGTCGTCAGT GTCCGGGTTA TCGAAAGGAA CTCAGCTCGG CCCTGTGGAT CTGCGAGTCG 
GCACAATCAG AGAGTCTGGC TAAAACTGCT GGAGACGGTC CCTCTACATC CTCTGATTCC 
ACCACAGCAG CTCCTCAGGA GTTCAGATGT CCTCCTCAGG CCAGTCACCT GATCTGCACC 
TGCTGTCTGC AGCCCATGCC GGACCGCCGC TTCGAGCACC TTCCCCCTCA GGTGTCCCCG 
CAGCACTGTT TGGTGTGCCA GAAACCGTTC TGTCACGTGT ACTGGGGCTG CCCGCGGATC 
GGCTGTCATG GCTGTTTAGC ACGTTTCAGC GAACTAAACC TCAATGATAA ATGTCTGGAT 
GGAGTTTTTA ATGGCAATCA ATACGAGTCG GAGGTCCTTC AGAATTATCT GAGCTGTCGA 
GGGATGAGCT GGAGGCATTT GCTGCAGGAC TCGCTGCAGG CTCTACAGCA GGGGCTCTAC 
CATCTCTCTG ATTATAGAAT CACTGCCAAC TCTTTTCTGT GCTACTGCTG TGGACTTCGC 
ACTTTTCGAG AGCTCGCATA CAAATACAGA GAGCGTATTC CTCCATCAGA GCTTCCAGAT 
GCTGTGACCA ACAGACCGAA CTGTTACTGG GGACGCAACT GTCGAACTCA AGTGAAAGCA 
CATCATGCAC TGAAGTTCAA CCACATATGT GAGCAGACCC GTTTTAAGAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001093485.1
CDS	148..2061
Translation	MTNQDSDQAWGKLVKVDASPGSEIVLINSECTVGRKKDCDLSFPANKLVSGNHCKITHDQNSGKVWLEDMSTNGTVINMSKVVKKQTHLLQNGDVIYFVYRKNEPEQNIAYVYQSITPQESASHDVEDAGREEDSDLTETESEPAPVEPVIVKPLPQSGHEDPQPSTSSSSLHFYNMPLSTCSDVSARKNPVSSSAVCKGDSTSSGSPAQTRLKWTCWTDGEPEEEMQRKRRKTDRDDPGFGSAHSDASADIPLRGASGKEKTEGATTDKMEESLTCIICQDLLYDCISVQPCMHTFCAACYSGWMERSSFCPTCRCPVERIRKNHILNNLVEAYLLQHPEKCRTEDDLRSMDARNKITQDMLQPKVERSFSDEEASSDYLFELSDNDSDISDMSQPYMMCRQCPGYRKELSSALWICESAQSESLAKTAGDGPSTSSDSTTAAPQEFRCPPQASHLICTCCLQPMPDRRFEHLPPQVSPQHCLVCQKPFCHVYWGCPRIGCHGCLARFSELNLNDKCLDGVFNGNQYESEVLQNYLSCRGMSWRHLLQDSLQALQQGLYHLSDYRITANSFLCYCCGLRTFRELAYKYRERIPPSELPDAVTNRPNCYWGRNCRTQVKAHHALKFNHICEQTRFKN

Target ORF information:

RefSeq Version	NM_001100015.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase (chfr), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001100015.1

ORF Insert Sequence:

ATGACAAATC AGGACAGCGA TCAAGCCTGG GGGAAACTGG TGAAAGTGGA CGCATCTCCA 
GGTTCGGAGA TAGTGCTTAT AAATAGTGAA TGCACCGTTG GGAGAAAGAA AGATTGTGAC 
CTCTCATTCC CAGCTAATAA ACTGGTCTCA GGAAATCATT GCAAAATCAC TCATGATCAA 
AACTCAGGAA AGGTGTGGCT GGAAGACATG AGCACCAACG GAACTGTGAT CAACATGTCC 
AAAGTAGTGA AGAAACAGAC GCACTTACTG CAGAACGGTG ATGTCATTTA CTTTGTGTAC 
AGGAAGAACG AGCCAGAGCA AAACATTGCT TATGTTTATC AGTCCATCAC GCCACAGGAA 
AGTGCCTCAC ATGATGTTGA AGATGCAGGG AGAGAAGAAG ATTCTGATCT GACAGAAACG 
GAGAGTGAAC CAGCACCAGT AGAGCCTGTT ATCGTGAAGC CTTTACCTCA GTCTGGACAT 
GAGGATCCTC AGCCATCTAC CTCCAGCTCT TCCTTACACT TCTACAACAT GCCATTGTCT 
ACCTGCTCTG ATGTGTCTGC AAGAAAGAAT CCAGTCAGTT CTTCAGCTGT TTGTAAAGGA 
GACTCTACTT CATCAGGAAG TCCCGCTCAA ACCCGGCTGA AGTGGACCTG CTGGACTGAT 
GGAGAGCCGG AGGAGGAAAT GCAGAGGAAA AGAAGAAAAA CTGACAGAGA TGATCCAGGG 
TTTGGATCTG CTCACAGTGA TGCTTCTGCT GACATTCCCC TCAGAGGAGC AAGTGGAAAA 
GAAAAAACTG AAGGAGCCAC AACAGACAAG ATGGAGGAAT CCCTCACATG CATCATCTGT 
CAGGACCTGC TATATGACTG CATCAGTGTT CAGCCTTGCA TGCATACGTT TTGTGCGGCC 
TGTTATTCTG GCTGGATGGA GCGTTCCTCT TTCTGTCCCA CCTGTCGATG TCCTGTGGAG 
AGGATCCGCA AAAACCACAT CCTCAACAAC CTGGTGGAGG CTTACCTTCT CCAGCACCCG 
GAGAAGTGTC GGACTGAAGA TGATTTGCGC AGTATGGATG CCAGAAACAA GATCACACAG 
GACATGCTGC AGCCGAAGGT CGAGCGATCG TTCTCAGACG AGGAGGCCAG TTCAGACTAT 
CTGTTTGAAC TCTCAGACAA TGACAGCGAC ATTTCTGACA TGAGCCAACC CTACATGATG 
TGTCGTCAGT GTCCGGGTTA TCGAAAGGAA CTCAGCTCGG CCCTGTGGAT CTGCGAGTCG 
GCACAATCAG AGAGTCTGGC TAAAACTGCT GGAGACGGTC CCTCTACATC CTCTGATTCC 
ACCACAGCAG CTCCTCAGGA GTTCAGATGT CCTCCTCAGG CCAGTCACCT GATCTGCACC 
TGCTGTCTGC AGCCCATGCC GGACCGCCGC TTCGAGCACC TTCCCCCTCA GGTGTCCCCG 
CAGCACTGTT TGGTGTGCCA GAAACCGTTC TGTCACGTGT ACTGGGGCTG CCCGCGGATC 
GGCTGTCATG GCTGTTTAGC ACGTTTCAGC GAACTAAACC TCAATGATAA ATGTCTGGAT 
GGAGTTTTTA ATGGCAATCA ATACGAGTCG GAGGTCCTTC AGAATTATCT GAGCTGTCGA 
GGGATGAGCT GGAGGCATTT GCTGCAGGAC TCGCTGCAGG CTCTACAGCA GGGGCTCTAC 
CATCTCTCTG ATTATAGAAT CACTGCCAAC TCTTTTCTGT GCTACTGCTG TGGACTTCGC 
ACTTTTCGAG AGCTCGCATA CAAATACAGA GAGCGTATTC CTCCATCAGA GCTTCCAGAT 
GCTGTGACCA ACAGACCGAA CTGTTACTGG GGACGCAACT GTCGAACTCA AGTGAAAGCA 
CATCATGCAC TGAAGTTCAA CCACATATGT GAGCAGACCC GTTTTAAGAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

chfr ( XM_017356138.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017211627.1 Danio rerio checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase (chfr), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa00246
Clone ID Related Accession (Same CDS sequence)	XM_017356138.1 , NM_001100015.1 , XM_017356138.2
Accession Version	XM_017356138.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1914bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	E3 ubiquitin-protein ligase CHFR isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAAATC AGGACAGCGA TCAAGCCTGG GGGAAACTGG TGAAAGTGGA CGCATCTCCA 
GGTTCGGAGA TAGTGCTTAT AAATAGTGAA TGCACCGTTG GGAGAAAGAA AGATTGTGAC 
CTCTCATTCC CAGCTAATAA ACTGGTCTCA GGAAATCATT GCAAAATCAC TCATGATCAA 
AACTCAGGAA AGGTGTGGCT GGAAGACATG AGCACCAACG GAACTGTGAT CAACATGTCC 
AAAGTAGTGA AGAAACAGAC GCACTTACTG CAGAACGGTG ATGTCATTTA CTTTGTGTAC 
AGGAAGAACG AGCCAGAGCA AAACATTGCT TATGTTTATC AGTCCATCAC GCCACAGGAA 
AGTGCCTCAC ATGATGTTGA AGATGCAGGG AGAGAAGAAG ATTCTGATCT GACAGAAACG 
GAGAGTGAAC CAGCACCAGT AGAGCCTGTT ATCGTGAAGC CTTTACCTCA GTCTGGACAT 
GAGGATCCTC AGCCATCTAC CTCCAGCTCT TCCTTACACT TCTACAACAT GCCATTGTCT 
ACCTGCTCTG ATGTGTCTGC AAGAAAGAAT CCAGTCAGTT CTTCAGCTGT TTGTAAAGGA 
GACTCTACTT CATCAGGAAG TCCCGCTCAA ACCCGGCTGA AGTGGACCTG CTGGACTGAT 
GGAGAGCCGG AGGAGGAAAT GCAGAGGAAA AGAAGAAAAA CTGACAGAGA TGATCCAGGG 
TTTGGATCTG CTCACAGTGA TGCTTCTGCT GACATTCCCC TCAGAGGAGC AAGTGGAAAA 
GAAAAAACTG AAGGAGCCAC AACAGACAAG ATGGAGGAAT CCCTCACATG CATCATCTGT 
CAGGACCTGC TATATGACTG CATCAGTGTT CAGCCTTGCA TGCATACGTT TTGTGCGGCC 
TGTTATTCTG GCTGGATGGA GCGTTCCTCT TTCTGTCCCA CCTGTCGATG TCCTGTGGAG 
AGGATCCGCA AAAACCACAT CCTCAACAAC CTGGTGGAGG CTTACCTTCT CCAGCACCCG 
GAGAAGTGTC GGACTGAAGA TGATTTGCGC AGTATGGATG CCAGAAACAA GATCACACAG 
GACATGCTGC AGCCGAAGGT CGAGCGATCG TTCTCAGACG AGGAGGCCAG TTCAGACTAT 
CTGTTTGAAC TCTCAGACAA TGACAGCGAC ATTTCTGACA TGAGCCAACC CTACATGATG 
TGTCGTCAGT GTCCGGGTTA TCGAAAGGAA CTCAGCTCGG CCCTGTGGAT CTGCGAGTCG 
GCACAATCAG AGAGTCTGGC TAAAACTGCT GGAGACGGTC CCTCTACATC CTCTGATTCC 
ACCACAGCAG CTCCTCAGGA GTTCAGATGT CCTCCTCAGG CCAGTCACCT GATCTGCACC 
TGCTGTCTGC AGCCCATGCC GGACCGCCGC TTCGAGCACC TTCCCCCTCA GGTGTCCCCG 
CAGCACTGTT TGGTGTGCCA GAAACCGTTC TGTCACGTGT ACTGGGGCTG CCCGCGGATC 
GGCTGTCATG GCTGTTTAGC ACGTTTCAGC GAACTAAACC TCAATGATAA ATGTCTGGAT 
GGAGTTTTTA ATGGCAATCA ATACGAGTCG GAGGTCCTTC AGAATTATCT GAGCTGTCGA 
GGGATGAGCT GGAGGCATTT GCTGCAGGAC TCGCTGCAGG CTCTACAGCA GGGGCTCTAC 
CATCTCTCTG ATTATAGAAT CACTGCCAAC TCTTTTCTGT GCTACTGCTG TGGACTTCGC 
ACTTTTCGAG AGCTCGCATA CAAATACAGA GAGCGTATTC CTCCATCAGA GCTTCCAGAT 
GCTGTGACCA ACAGACCGAA CTGTTACTGG GGACGCAACT GTCGAACTCA AGTGAAAGCA 
CATCATGCAC TGAAGTTCAA CCACATATGT GAGCAGACCC GTTTTAAGAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017211627.1
CDS	216..2129
Translation	MTNQDSDQAWGKLVKVDASPGSEIVLINSECTVGRKKDCDLSFPANKLVSGNHCKITHDQNSGKVWLEDMSTNGTVINMSKVVKKQTHLLQNGDVIYFVYRKNEPEQNIAYVYQSITPQESASHDVEDAGREEDSDLTETESEPAPVEPVIVKPLPQSGHEDPQPSTSSSSLHFYNMPLSTCSDVSARKNPVSSSAVCKGDSTSSGSPAQTRLKWTCWTDGEPEEEMQRKRRKTDRDDPGFGSAHSDASADIPLRGASGKEKTEGATTDKMEESLTCIICQDLLYDCISVQPCMHTFCAACYSGWMERSSFCPTCRCPVERIRKNHILNNLVEAYLLQHPEKCRTEDDLRSMDARNKITQDMLQPKVERSFSDEEASSDYLFELSDNDSDISDMSQPYMMCRQCPGYRKELSSALWICESAQSESLAKTAGDGPSTSSDSTTAAPQEFRCPPQASHLICTCCLQPMPDRRFEHLPPQVSPQHCLVCQKPFCHVYWGCPRIGCHGCLARFSELNLNDKCLDGVFNGNQYESEVLQNYLSCRGMSWRHLLQDSLQALQQGLYHLSDYRITANSFLCYCCGLRTFRELAYKYRERIPPSELPDAVTNRPNCYWGRNCRTQVKAHHALKFNHICEQTRFKN

Target ORF information:

RefSeq Version	XM_017356138.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase (chfr), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356138.1

ORF Insert Sequence:

ATGACAAATC AGGACAGCGA TCAAGCCTGG GGGAAACTGG TGAAAGTGGA CGCATCTCCA 
GGTTCGGAGA TAGTGCTTAT AAATAGTGAA TGCACCGTTG GGAGAAAGAA AGATTGTGAC 
CTCTCATTCC CAGCTAATAA ACTGGTCTCA GGAAATCATT GCAAAATCAC TCATGATCAA 
AACTCAGGAA AGGTGTGGCT GGAAGACATG AGCACCAACG GAACTGTGAT CAACATGTCC 
AAAGTAGTGA AGAAACAGAC GCACTTACTG CAGAACGGTG ATGTCATTTA CTTTGTGTAC 
AGGAAGAACG AGCCAGAGCA AAACATTGCT TATGTTTATC AGTCCATCAC GCCACAGGAA 
AGTGCCTCAC ATGATGTTGA AGATGCAGGG AGAGAAGAAG ATTCTGATCT GACAGAAACG 
GAGAGTGAAC CAGCACCAGT AGAGCCTGTT ATCGTGAAGC CTTTACCTCA GTCTGGACAT 
GAGGATCCTC AGCCATCTAC CTCCAGCTCT TCCTTACACT TCTACAACAT GCCATTGTCT 
ACCTGCTCTG ATGTGTCTGC AAGAAAGAAT CCAGTCAGTT CTTCAGCTGT TTGTAAAGGA 
GACTCTACTT CATCAGGAAG TCCCGCTCAA ACCCGGCTGA AGTGGACCTG CTGGACTGAT 
GGAGAGCCGG AGGAGGAAAT GCAGAGGAAA AGAAGAAAAA CTGACAGAGA TGATCCAGGG 
TTTGGATCTG CTCACAGTGA TGCTTCTGCT GACATTCCCC TCAGAGGAGC AAGTGGAAAA 
GAAAAAACTG AAGGAGCCAC AACAGACAAG ATGGAGGAAT CCCTCACATG CATCATCTGT 
CAGGACCTGC TATATGACTG CATCAGTGTT CAGCCTTGCA TGCATACGTT TTGTGCGGCC 
TGTTATTCTG GCTGGATGGA GCGTTCCTCT TTCTGTCCCA CCTGTCGATG TCCTGTGGAG 
AGGATCCGCA AAAACCACAT CCTCAACAAC CTGGTGGAGG CTTACCTTCT CCAGCACCCG 
GAGAAGTGTC GGACTGAAGA TGATTTGCGC AGTATGGATG CCAGAAACAA GATCACACAG 
GACATGCTGC AGCCGAAGGT CGAGCGATCG TTCTCAGACG AGGAGGCCAG TTCAGACTAT 
CTGTTTGAAC TCTCAGACAA TGACAGCGAC ATTTCTGACA TGAGCCAACC CTACATGATG 
TGTCGTCAGT GTCCGGGTTA TCGAAAGGAA CTCAGCTCGG CCCTGTGGAT CTGCGAGTCG 
GCACAATCAG AGAGTCTGGC TAAAACTGCT GGAGACGGTC CCTCTACATC CTCTGATTCC 
ACCACAGCAG CTCCTCAGGA GTTCAGATGT CCTCCTCAGG CCAGTCACCT GATCTGCACC 
TGCTGTCTGC AGCCCATGCC GGACCGCCGC TTCGAGCACC TTCCCCCTCA GGTGTCCCCG 
CAGCACTGTT TGGTGTGCCA GAAACCGTTC TGTCACGTGT ACTGGGGCTG CCCGCGGATC 
GGCTGTCATG GCTGTTTAGC ACGTTTCAGC GAACTAAACC TCAATGATAA ATGTCTGGAT 
GGAGTTTTTA ATGGCAATCA ATACGAGTCG GAGGTCCTTC AGAATTATCT GAGCTGTCGA 
GGGATGAGCT GGAGGCATTT GCTGCAGGAC TCGCTGCAGG CTCTACAGCA GGGGCTCTAC 
CATCTCTCTG ATTATAGAAT CACTGCCAAC TCTTTTCTGT GCTACTGCTG TGGACTTCGC 
ACTTTTCGAG AGCTCGCATA CAAATACAGA GAGCGTATTC CTCCATCAGA GCTTCCAGAT 
GCTGTGACCA ACAGACCGAA CTGTTACTGG GGACGCAACT GTCGAACTCA AGTGAAAGCA 
CATCATGCAC TGAAGTTCAA CCACATATGT GAGCAGACCC GTTTTAAGAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

chfr ( XM_017356138.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017211627.1 Danio rerio checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase (chfr), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa00246
Clone ID Related Accession (Same CDS sequence)	XM_017356138.1 , NM_001100015.1 , XM_017356138.2
Accession Version	XM_017356138.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1914bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase CHFR isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017356138.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAAATC AGGACAGCGA TCAAGCCTGG GGGAAACTGG TGAAAGTGGA CGCATCTCCA 
GGTTCGGAGA TAGTGCTTAT AAATAGTGAA TGCACCGTTG GGAGAAAGAA AGATTGTGAC 
CTCTCATTCC CAGCTAATAA ACTGGTCTCA GGAAATCATT GCAAAATCAC TCATGATCAA 
AACTCAGGAA AGGTGTGGCT GGAAGACATG AGCACCAACG GAACTGTGAT CAACATGTCC 
AAAGTAGTGA AGAAACAGAC GCACTTACTG CAGAACGGTG ATGTCATTTA CTTTGTGTAC 
AGGAAGAACG AGCCAGAGCA AAACATTGCT TATGTTTATC AGTCCATCAC GCCACAGGAA 
AGTGCCTCAC ATGATGTTGA AGATGCAGGG AGAGAAGAAG ATTCTGATCT GACAGAAACG 
GAGAGTGAAC CAGCACCAGT AGAGCCTGTT ATCGTGAAGC CTTTACCTCA GTCTGGACAT 
GAGGATCCTC AGCCATCTAC CTCCAGCTCT TCCTTACACT TCTACAACAT GCCATTGTCT 
ACCTGCTCTG ATGTGTCTGC AAGAAAGAAT CCAGTCAGTT CTTCAGCTGT TTGTAAAGGA 
GACTCTACTT CATCAGGAAG TCCCGCTCAA ACCCGGCTGA AGTGGACCTG CTGGACTGAT 
GGAGAGCCGG AGGAGGAAAT GCAGAGGAAA AGAAGAAAAA CTGACAGAGA TGATCCAGGG 
TTTGGATCTG CTCACAGTGA TGCTTCTGCT GACATTCCCC TCAGAGGAGC AAGTGGAAAA 
GAAAAAACTG AAGGAGCCAC AACAGACAAG ATGGAGGAAT CCCTCACATG CATCATCTGT 
CAGGACCTGC TATATGACTG CATCAGTGTT CAGCCTTGCA TGCATACGTT TTGTGCGGCC 
TGTTATTCTG GCTGGATGGA GCGTTCCTCT TTCTGTCCCA CCTGTCGATG TCCTGTGGAG 
AGGATCCGCA AAAACCACAT CCTCAACAAC CTGGTGGAGG CTTACCTTCT CCAGCACCCG 
GAGAAGTGTC GGACTGAAGA TGATTTGCGC AGTATGGATG CCAGAAACAA GATCACACAG 
GACATGCTGC AGCCGAAGGT CGAGCGATCG TTCTCAGACG AGGAGGCCAG TTCAGACTAT 
CTGTTTGAAC TCTCAGACAA TGACAGCGAC ATTTCTGACA TGAGCCAACC CTACATGATG 
TGTCGTCAGT GTCCGGGTTA TCGAAAGGAA CTCAGCTCGG CCCTGTGGAT CTGCGAGTCG 
GCACAATCAG AGAGTCTGGC TAAAACTGCT GGAGACGGTC CCTCTACATC CTCTGATTCC 
ACCACAGCAG CTCCTCAGGA GTTCAGATGT CCTCCTCAGG CCAGTCACCT GATCTGCACC 
TGCTGTCTGC AGCCCATGCC GGACCGCCGC TTCGAGCACC TTCCCCCTCA GGTGTCCCCG 
CAGCACTGTT TGGTGTGCCA GAAACCGTTC TGTCACGTGT ACTGGGGCTG CCCGCGGATC 
GGCTGTCATG GCTGTTTAGC ACGTTTCAGC GAACTAAACC TCAATGATAA ATGTCTGGAT 
GGAGTTTTTA ATGGCAATCA ATACGAGTCG GAGGTCCTTC AGAATTATCT GAGCTGTCGA 
GGGATGAGCT GGAGGCATTT GCTGCAGGAC TCGCTGCAGG CTCTACAGCA GGGGCTCTAC 
CATCTCTCTG ATTATAGAAT CACTGCCAAC TCTTTTCTGT GCTACTGCTG TGGACTTCGC 
ACTTTTCGAG AGCTCGCATA CAAATACAGA GAGCGTATTC CTCCATCAGA GCTTCCAGAT 
GCTGTGACCA ACAGACCGAA CTGTTACTGG GGACGCAACT GTCGAACTCA AGTGAAAGCA 
CATCATGCAC TGAAGTTCAA CCACATATGT GAGCAGACCC GTTTTAAGAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017211627.1
CDS	176..2089
Translation	MTNQDSDQAWGKLVKVDASPGSEIVLINSECTVGRKKDCDLSFPANKLVSGNHCKITHDQNSGKVWLEDMSTNGTVINMSKVVKKQTHLLQNGDVIYFVYRKNEPEQNIAYVYQSITPQESASHDVEDAGREEDSDLTETESEPAPVEPVIVKPLPQSGHEDPQPSTSSSSLHFYNMPLSTCSDVSARKNPVSSSAVCKGDSTSSGSPAQTRLKWTCWTDGEPEEEMQRKRRKTDRDDPGFGSAHSDASADIPLRGASGKEKTEGATTDKMEESLTCIICQDLLYDCISVQPCMHTFCAACYSGWMERSSFCPTCRCPVERIRKNHILNNLVEAYLLQHPEKCRTEDDLRSMDARNKITQDMLQPKVERSFSDEEASSDYLFELSDNDSDISDMSQPYMMCRQCPGYRKELSSALWICESAQSESLAKTAGDGPSTSSDSTTAAPQEFRCPPQASHLICTCCLQPMPDRRFEHLPPQVSPQHCLVCQKPFCHVYWGCPRIGCHGCLARFSELNLNDKCLDGVFNGNQYESEVLQNYLSCRGMSWRHLLQDSLQALQQGLYHLSDYRITANSFLCYCCGLRTFRELAYKYRERIPPSELPDAVTNRPNCYWGRNCRTQVKAHHALKFNHICEQTRFKN

Target ORF information:

RefSeq Version	XM_017356138.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase (chfr), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356138.2

ORF Insert Sequence:

ATGACAAATC AGGACAGCGA TCAAGCCTGG GGGAAACTGG TGAAAGTGGA CGCATCTCCA 
GGTTCGGAGA TAGTGCTTAT AAATAGTGAA TGCACCGTTG GGAGAAAGAA AGATTGTGAC 
CTCTCATTCC CAGCTAATAA ACTGGTCTCA GGAAATCATT GCAAAATCAC TCATGATCAA 
AACTCAGGAA AGGTGTGGCT GGAAGACATG AGCACCAACG GAACTGTGAT CAACATGTCC 
AAAGTAGTGA AGAAACAGAC GCACTTACTG CAGAACGGTG ATGTCATTTA CTTTGTGTAC 
AGGAAGAACG AGCCAGAGCA AAACATTGCT TATGTTTATC AGTCCATCAC GCCACAGGAA 
AGTGCCTCAC ATGATGTTGA AGATGCAGGG AGAGAAGAAG ATTCTGATCT GACAGAAACG 
GAGAGTGAAC CAGCACCAGT AGAGCCTGTT ATCGTGAAGC CTTTACCTCA GTCTGGACAT 
GAGGATCCTC AGCCATCTAC CTCCAGCTCT TCCTTACACT TCTACAACAT GCCATTGTCT 
ACCTGCTCTG ATGTGTCTGC AAGAAAGAAT CCAGTCAGTT CTTCAGCTGT TTGTAAAGGA 
GACTCTACTT CATCAGGAAG TCCCGCTCAA ACCCGGCTGA AGTGGACCTG CTGGACTGAT 
GGAGAGCCGG AGGAGGAAAT GCAGAGGAAA AGAAGAAAAA CTGACAGAGA TGATCCAGGG 
TTTGGATCTG CTCACAGTGA TGCTTCTGCT GACATTCCCC TCAGAGGAGC AAGTGGAAAA 
GAAAAAACTG AAGGAGCCAC AACAGACAAG ATGGAGGAAT CCCTCACATG CATCATCTGT 
CAGGACCTGC TATATGACTG CATCAGTGTT CAGCCTTGCA TGCATACGTT TTGTGCGGCC 
TGTTATTCTG GCTGGATGGA GCGTTCCTCT TTCTGTCCCA CCTGTCGATG TCCTGTGGAG 
AGGATCCGCA AAAACCACAT CCTCAACAAC CTGGTGGAGG CTTACCTTCT CCAGCACCCG 
GAGAAGTGTC GGACTGAAGA TGATTTGCGC AGTATGGATG CCAGAAACAA GATCACACAG 
GACATGCTGC AGCCGAAGGT CGAGCGATCG TTCTCAGACG AGGAGGCCAG TTCAGACTAT 
CTGTTTGAAC TCTCAGACAA TGACAGCGAC ATTTCTGACA TGAGCCAACC CTACATGATG 
TGTCGTCAGT GTCCGGGTTA TCGAAAGGAA CTCAGCTCGG CCCTGTGGAT CTGCGAGTCG 
GCACAATCAG AGAGTCTGGC TAAAACTGCT GGAGACGGTC CCTCTACATC CTCTGATTCC 
ACCACAGCAG CTCCTCAGGA GTTCAGATGT CCTCCTCAGG CCAGTCACCT GATCTGCACC 
TGCTGTCTGC AGCCCATGCC GGACCGCCGC TTCGAGCACC TTCCCCCTCA GGTGTCCCCG 
CAGCACTGTT TGGTGTGCCA GAAACCGTTC TGTCACGTGT ACTGGGGCTG CCCGCGGATC 
GGCTGTCATG GCTGTTTAGC ACGTTTCAGC GAACTAAACC TCAATGATAA ATGTCTGGAT 
GGAGTTTTTA ATGGCAATCA ATACGAGTCG GAGGTCCTTC AGAATTATCT GAGCTGTCGA 
GGGATGAGCT GGAGGCATTT GCTGCAGGAC TCGCTGCAGG CTCTACAGCA GGGGCTCTAC 
CATCTCTCTG ATTATAGAAT CACTGCCAAC TCTTTTCTGT GCTACTGCTG TGGACTTCGC 
ACTTTTCGAG AGCTCGCATA CAAATACAGA GAGCGTATTC CTCCATCAGA GCTTCCAGAT 
GCTGTGACCA ACAGACCGAA CTGTTACTGG GGACGCAACT GTCGAACTCA AGTGAAAGCA 
CATCATGCAC TGAAGTTCAA CCACATATGT GAGCAGACCC GTTTTAAGAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

chfr ( XM_017356139.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017211628.1 Danio rerio checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase (chfr), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa10198
Clone ID Related Accession (Same CDS sequence)	XM_017356139.1 , XM_017356139.2 , XM_021476266.1
Accession Version	XM_017356139.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1911bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	E3 ubiquitin-protein ligase CHFR isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAAATC AGGACAGCGA TCAAGCCTGG GGGAAACTGG TGAAAGTGGA CGCATCTCCA 
GGTTCGGAGA TAGTGCTTAT AAATAGTGAA TGCACCGTTG GGAGAAAGAA AGATTGTGAC 
CTCTCATTCC CAGCTAATAA ACTGGTCTCA GGAAATCATT GCAAAATCAC TCATGATCAA 
AACTCAGGAA AGGTGTGGCT GGAAGACATG AGCACCAACG GAACTGTGAT CAACATGTCC 
AAAGTAGTGA AGAAACAGAC GCACTTACTG CAGAACGGTG ATGTCATTTA CTTTGTGTAC 
AGGAAGAACG AGCCAGAGCA AAACATTGCT TATGTTTATC AGTCCATCAC GCCACAGGAA 
AGTGCCTCAC ATGATGTTGA AGATGCAGGG AGAGAAGAAG ATTCTGATCT GACAGAAACG 
GAGAGTGAAC CAGCACCAGT AGAGCCTGTT ATCGTGAAGC CTTTACCTCA GTCTGGACAT 
GAGGATCCTC AGCCATCTAC CTCCAGCTCT TCCTTACACT TCTACAACAT GCCATTGTCT 
ACCTGCTCTG ATGTGTCTGC AAGAAAGAAT CCAGTCAGTT CTTCAGCTGT TTGTAAAGGA 
GACTCTACTT CATCAGGAAG TCCCGCTCAA ACCCGGCTGA AGTGGACCTG CTGGACTGAT 
GGAGAGCCGG AGGAGGAAAT GCAGAGGAAA AGAAGAAAAA CTGACAGAGA TGATCCAGGG 
TTTGGATCTG CTCACAGTGA TGCTTCTGCT GACATTCCCC TCAGAGGAGC AAGTGGAAAA 
GAAAAAACTG AAGGAGCCAC AACAGACAAG ATGGAGGAAT CCCTCACATG CATCATCTGT 
CAGGACCTGC TATATGACTG CATCAGTGTT CAGCCTTGCA TGCATACGTT TTGTGCGGCC 
TGTTATTCTG GCTGGATGGA GCGTTCCTCT TTCTGTCCCA CCTGTCGATG TCCTGTGGAG 
AGGATCCGCA AAAACCACAT CCTCAACAAC CTGGTGGAGG CTTACCTTCT CCAGCACCCG 
GAGAAGTGTC GGACTGAAGA TGATTTGCGC AGTATGGATG CCAGAAACAA GATCACACAG 
GACATGCTGC AGCCGAAGGT CGAGCGATCG TTCTCAGACG AGGAGGCCAG TTCAGACTAT 
CTGTTTGAAC TCTCAGACAA TGACAGCGAC ATTTCTGACA TGAGCCAACC CTACATGATG 
TGTCGTCAGT GTCCGGGTTA TCGAAAGGAA CTCAGCTCGG CCCTGTGGAT CTGCGAGTCG 
GCACAATCAG AGAGTCTGGC TAAAACTGCT GGAGACGGTC CCTCTACATC CTCTGATTCC 
ACCACAGCTC CTCAGGAGTT CAGATGTCCT CCTCAGGCCA GTCACCTGAT CTGCACCTGC 
TGTCTGCAGC CCATGCCGGA CCGCCGCTTC GAGCACCTTC CCCCTCAGGT GTCCCCGCAG 
CACTGTTTGG TGTGCCAGAA ACCGTTCTGT CACGTGTACT GGGGCTGCCC GCGGATCGGC 
TGTCATGGCT GTTTAGCACG TTTCAGCGAA CTAAACCTCA ATGATAAATG TCTGGATGGA 
GTTTTTAATG GCAATCAATA CGAGTCGGAG GTCCTTCAGA ATTATCTGAG CTGTCGAGGG 
ATGAGCTGGA GGCATTTGCT GCAGGACTCG CTGCAGGCTC TACAGCAGGG GCTCTACCAT 
CTCTCTGATT ATAGAATCAC TGCCAACTCT TTTCTGTGCT ACTGCTGTGG ACTTCGCACT 
TTTCGAGAGC TCGCATACAA ATACAGAGAG CGTATTCCTC CATCAGAGCT TCCAGATGCT 
GTGACCAACA GACCGAACTG TTACTGGGGA CGCAACTGTC GAACTCAAGT GAAAGCACAT 
CATGCACTGA AGTTCAACCA CATATGTGAG CAGACCCGTT TTAAGAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017211628.1
CDS	216..2126
Translation	MTNQDSDQAWGKLVKVDASPGSEIVLINSECTVGRKKDCDLSFPANKLVSGNHCKITHDQNSGKVWLEDMSTNGTVINMSKVVKKQTHLLQNGDVIYFVYRKNEPEQNIAYVYQSITPQESASHDVEDAGREEDSDLTETESEPAPVEPVIVKPLPQSGHEDPQPSTSSSSLHFYNMPLSTCSDVSARKNPVSSSAVCKGDSTSSGSPAQTRLKWTCWTDGEPEEEMQRKRRKTDRDDPGFGSAHSDASADIPLRGASGKEKTEGATTDKMEESLTCIICQDLLYDCISVQPCMHTFCAACYSGWMERSSFCPTCRCPVERIRKNHILNNLVEAYLLQHPEKCRTEDDLRSMDARNKITQDMLQPKVERSFSDEEASSDYLFELSDNDSDISDMSQPYMMCRQCPGYRKELSSALWICESAQSESLAKTAGDGPSTSSDSTTAPQEFRCPPQASHLICTCCLQPMPDRRFEHLPPQVSPQHCLVCQKPFCHVYWGCPRIGCHGCLARFSELNLNDKCLDGVFNGNQYESEVLQNYLSCRGMSWRHLLQDSLQALQQGLYHLSDYRITANSFLCYCCGLRTFRELAYKYRERIPPSELPDAVTNRPNCYWGRNCRTQVKAHHALKFNHICEQTRFKN

Target ORF information:

RefSeq Version	XM_017356139.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase (chfr), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356139.1

ORF Insert Sequence:

ATGACAAATC AGGACAGCGA TCAAGCCTGG GGGAAACTGG TGAAAGTGGA CGCATCTCCA 
GGTTCGGAGA TAGTGCTTAT AAATAGTGAA TGCACCGTTG GGAGAAAGAA AGATTGTGAC 
CTCTCATTCC CAGCTAATAA ACTGGTCTCA GGAAATCATT GCAAAATCAC TCATGATCAA 
AACTCAGGAA AGGTGTGGCT GGAAGACATG AGCACCAACG GAACTGTGAT CAACATGTCC 
AAAGTAGTGA AGAAACAGAC GCACTTACTG CAGAACGGTG ATGTCATTTA CTTTGTGTAC 
AGGAAGAACG AGCCAGAGCA AAACATTGCT TATGTTTATC AGTCCATCAC GCCACAGGAA 
AGTGCCTCAC ATGATGTTGA AGATGCAGGG AGAGAAGAAG ATTCTGATCT GACAGAAACG 
GAGAGTGAAC CAGCACCAGT AGAGCCTGTT ATCGTGAAGC CTTTACCTCA GTCTGGACAT 
GAGGATCCTC AGCCATCTAC CTCCAGCTCT TCCTTACACT TCTACAACAT GCCATTGTCT 
ACCTGCTCTG ATGTGTCTGC AAGAAAGAAT CCAGTCAGTT CTTCAGCTGT TTGTAAAGGA 
GACTCTACTT CATCAGGAAG TCCCGCTCAA ACCCGGCTGA AGTGGACCTG CTGGACTGAT 
GGAGAGCCGG AGGAGGAAAT GCAGAGGAAA AGAAGAAAAA CTGACAGAGA TGATCCAGGG 
TTTGGATCTG CTCACAGTGA TGCTTCTGCT GACATTCCCC TCAGAGGAGC AAGTGGAAAA 
GAAAAAACTG AAGGAGCCAC AACAGACAAG ATGGAGGAAT CCCTCACATG CATCATCTGT 
CAGGACCTGC TATATGACTG CATCAGTGTT CAGCCTTGCA TGCATACGTT TTGTGCGGCC 
TGTTATTCTG GCTGGATGGA GCGTTCCTCT TTCTGTCCCA CCTGTCGATG TCCTGTGGAG 
AGGATCCGCA AAAACCACAT CCTCAACAAC CTGGTGGAGG CTTACCTTCT CCAGCACCCG 
GAGAAGTGTC GGACTGAAGA TGATTTGCGC AGTATGGATG CCAGAAACAA GATCACACAG 
GACATGCTGC AGCCGAAGGT CGAGCGATCG TTCTCAGACG AGGAGGCCAG TTCAGACTAT 
CTGTTTGAAC TCTCAGACAA TGACAGCGAC ATTTCTGACA TGAGCCAACC CTACATGATG 
TGTCGTCAGT GTCCGGGTTA TCGAAAGGAA CTCAGCTCGG CCCTGTGGAT CTGCGAGTCG 
GCACAATCAG AGAGTCTGGC TAAAACTGCT GGAGACGGTC CCTCTACATC CTCTGATTCC 
ACCACAGCTC CTCAGGAGTT CAGATGTCCT CCTCAGGCCA GTCACCTGAT CTGCACCTGC 
TGTCTGCAGC CCATGCCGGA CCGCCGCTTC GAGCACCTTC CCCCTCAGGT GTCCCCGCAG 
CACTGTTTGG TGTGCCAGAA ACCGTTCTGT CACGTGTACT GGGGCTGCCC GCGGATCGGC 
TGTCATGGCT GTTTAGCACG TTTCAGCGAA CTAAACCTCA ATGATAAATG TCTGGATGGA 
GTTTTTAATG GCAATCAATA CGAGTCGGAG GTCCTTCAGA ATTATCTGAG CTGTCGAGGG 
ATGAGCTGGA GGCATTTGCT GCAGGACTCG CTGCAGGCTC TACAGCAGGG GCTCTACCAT 
CTCTCTGATT ATAGAATCAC TGCCAACTCT TTTCTGTGCT ACTGCTGTGG ACTTCGCACT 
TTTCGAGAGC TCGCATACAA ATACAGAGAG CGTATTCCTC CATCAGAGCT TCCAGATGCT 
GTGACCAACA GACCGAACTG TTACTGGGGA CGCAACTGTC GAACTCAAGT GAAAGCACAT 
CATGCACTGA AGTTCAACCA CATATGTGAG CAGACCCGTT TTAAGAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

chfr ( XM_017356139.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017211628.1 Danio rerio checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase (chfr), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa10198
Clone ID Related Accession (Same CDS sequence)	XM_017356139.1 , XM_017356139.2 , XM_021476266.1
Accession Version	XM_017356139.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1911bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase CHFR isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017356139.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAAATC AGGACAGCGA TCAAGCCTGG GGGAAACTGG TGAAAGTGGA CGCATCTCCA 
GGTTCGGAGA TAGTGCTTAT AAATAGTGAA TGCACCGTTG GGAGAAAGAA AGATTGTGAC 
CTCTCATTCC CAGCTAATAA ACTGGTCTCA GGAAATCATT GCAAAATCAC TCATGATCAA 
AACTCAGGAA AGGTGTGGCT GGAAGACATG AGCACCAACG GAACTGTGAT CAACATGTCC 
AAAGTAGTGA AGAAACAGAC GCACTTACTG CAGAACGGTG ATGTCATTTA CTTTGTGTAC 
AGGAAGAACG AGCCAGAGCA AAACATTGCT TATGTTTATC AGTCCATCAC GCCACAGGAA 
AGTGCCTCAC ATGATGTTGA AGATGCAGGG AGAGAAGAAG ATTCTGATCT GACAGAAACG 
GAGAGTGAAC CAGCACCAGT AGAGCCTGTT ATCGTGAAGC CTTTACCTCA GTCTGGACAT 
GAGGATCCTC AGCCATCTAC CTCCAGCTCT TCCTTACACT TCTACAACAT GCCATTGTCT 
ACCTGCTCTG ATGTGTCTGC AAGAAAGAAT CCAGTCAGTT CTTCAGCTGT TTGTAAAGGA 
GACTCTACTT CATCAGGAAG TCCCGCTCAA ACCCGGCTGA AGTGGACCTG CTGGACTGAT 
GGAGAGCCGG AGGAGGAAAT GCAGAGGAAA AGAAGAAAAA CTGACAGAGA TGATCCAGGG 
TTTGGATCTG CTCACAGTGA TGCTTCTGCT GACATTCCCC TCAGAGGAGC AAGTGGAAAA 
GAAAAAACTG AAGGAGCCAC AACAGACAAG ATGGAGGAAT CCCTCACATG CATCATCTGT 
CAGGACCTGC TATATGACTG CATCAGTGTT CAGCCTTGCA TGCATACGTT TTGTGCGGCC 
TGTTATTCTG GCTGGATGGA GCGTTCCTCT TTCTGTCCCA CCTGTCGATG TCCTGTGGAG 
AGGATCCGCA AAAACCACAT CCTCAACAAC CTGGTGGAGG CTTACCTTCT CCAGCACCCG 
GAGAAGTGTC GGACTGAAGA TGATTTGCGC AGTATGGATG CCAGAAACAA GATCACACAG 
GACATGCTGC AGCCGAAGGT CGAGCGATCG TTCTCAGACG AGGAGGCCAG TTCAGACTAT 
CTGTTTGAAC TCTCAGACAA TGACAGCGAC ATTTCTGACA TGAGCCAACC CTACATGATG 
TGTCGTCAGT GTCCGGGTTA TCGAAAGGAA CTCAGCTCGG CCCTGTGGAT CTGCGAGTCG 
GCACAATCAG AGAGTCTGGC TAAAACTGCT GGAGACGGTC CCTCTACATC CTCTGATTCC 
ACCACAGCTC CTCAGGAGTT CAGATGTCCT CCTCAGGCCA GTCACCTGAT CTGCACCTGC 
TGTCTGCAGC CCATGCCGGA CCGCCGCTTC GAGCACCTTC CCCCTCAGGT GTCCCCGCAG 
CACTGTTTGG TGTGCCAGAA ACCGTTCTGT CACGTGTACT GGGGCTGCCC GCGGATCGGC 
TGTCATGGCT GTTTAGCACG TTTCAGCGAA CTAAACCTCA ATGATAAATG TCTGGATGGA 
GTTTTTAATG GCAATCAATA CGAGTCGGAG GTCCTTCAGA ATTATCTGAG CTGTCGAGGG 
ATGAGCTGGA GGCATTTGCT GCAGGACTCG CTGCAGGCTC TACAGCAGGG GCTCTACCAT 
CTCTCTGATT ATAGAATCAC TGCCAACTCT TTTCTGTGCT ACTGCTGTGG ACTTCGCACT 
TTTCGAGAGC TCGCATACAA ATACAGAGAG CGTATTCCTC CATCAGAGCT TCCAGATGCT 
GTGACCAACA GACCGAACTG TTACTGGGGA CGCAACTGTC GAACTCAAGT GAAAGCACAT 
CATGCACTGA AGTTCAACCA CATATGTGAG CAGACCCGTT TTAAGAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017211628.1
CDS	176..2086
Translation	MTNQDSDQAWGKLVKVDASPGSEIVLINSECTVGRKKDCDLSFPANKLVSGNHCKITHDQNSGKVWLEDMSTNGTVINMSKVVKKQTHLLQNGDVIYFVYRKNEPEQNIAYVYQSITPQESASHDVEDAGREEDSDLTETESEPAPVEPVIVKPLPQSGHEDPQPSTSSSSLHFYNMPLSTCSDVSARKNPVSSSAVCKGDSTSSGSPAQTRLKWTCWTDGEPEEEMQRKRRKTDRDDPGFGSAHSDASADIPLRGASGKEKTEGATTDKMEESLTCIICQDLLYDCISVQPCMHTFCAACYSGWMERSSFCPTCRCPVERIRKNHILNNLVEAYLLQHPEKCRTEDDLRSMDARNKITQDMLQPKVERSFSDEEASSDYLFELSDNDSDISDMSQPYMMCRQCPGYRKELSSALWICESAQSESLAKTAGDGPSTSSDSTTAPQEFRCPPQASHLICTCCLQPMPDRRFEHLPPQVSPQHCLVCQKPFCHVYWGCPRIGCHGCLARFSELNLNDKCLDGVFNGNQYESEVLQNYLSCRGMSWRHLLQDSLQALQQGLYHLSDYRITANSFLCYCCGLRTFRELAYKYRERIPPSELPDAVTNRPNCYWGRNCRTQVKAHHALKFNHICEQTRFKN

Target ORF information:

RefSeq Version	XM_017356139.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase (chfr), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356139.2

ORF Insert Sequence:

ATGACAAATC AGGACAGCGA TCAAGCCTGG GGGAAACTGG TGAAAGTGGA CGCATCTCCA 
GGTTCGGAGA TAGTGCTTAT AAATAGTGAA TGCACCGTTG GGAGAAAGAA AGATTGTGAC 
CTCTCATTCC CAGCTAATAA ACTGGTCTCA GGAAATCATT GCAAAATCAC TCATGATCAA 
AACTCAGGAA AGGTGTGGCT GGAAGACATG AGCACCAACG GAACTGTGAT CAACATGTCC 
AAAGTAGTGA AGAAACAGAC GCACTTACTG CAGAACGGTG ATGTCATTTA CTTTGTGTAC 
AGGAAGAACG AGCCAGAGCA AAACATTGCT TATGTTTATC AGTCCATCAC GCCACAGGAA 
AGTGCCTCAC ATGATGTTGA AGATGCAGGG AGAGAAGAAG ATTCTGATCT GACAGAAACG 
GAGAGTGAAC CAGCACCAGT AGAGCCTGTT ATCGTGAAGC CTTTACCTCA GTCTGGACAT 
GAGGATCCTC AGCCATCTAC CTCCAGCTCT TCCTTACACT TCTACAACAT GCCATTGTCT 
ACCTGCTCTG ATGTGTCTGC AAGAAAGAAT CCAGTCAGTT CTTCAGCTGT TTGTAAAGGA 
GACTCTACTT CATCAGGAAG TCCCGCTCAA ACCCGGCTGA AGTGGACCTG CTGGACTGAT 
GGAGAGCCGG AGGAGGAAAT GCAGAGGAAA AGAAGAAAAA CTGACAGAGA TGATCCAGGG 
TTTGGATCTG CTCACAGTGA TGCTTCTGCT GACATTCCCC TCAGAGGAGC AAGTGGAAAA 
GAAAAAACTG AAGGAGCCAC AACAGACAAG ATGGAGGAAT CCCTCACATG CATCATCTGT 
CAGGACCTGC TATATGACTG CATCAGTGTT CAGCCTTGCA TGCATACGTT TTGTGCGGCC 
TGTTATTCTG GCTGGATGGA GCGTTCCTCT TTCTGTCCCA CCTGTCGATG TCCTGTGGAG 
AGGATCCGCA AAAACCACAT CCTCAACAAC CTGGTGGAGG CTTACCTTCT CCAGCACCCG 
GAGAAGTGTC GGACTGAAGA TGATTTGCGC AGTATGGATG CCAGAAACAA GATCACACAG 
GACATGCTGC AGCCGAAGGT CGAGCGATCG TTCTCAGACG AGGAGGCCAG TTCAGACTAT 
CTGTTTGAAC TCTCAGACAA TGACAGCGAC ATTTCTGACA TGAGCCAACC CTACATGATG 
TGTCGTCAGT GTCCGGGTTA TCGAAAGGAA CTCAGCTCGG CCCTGTGGAT CTGCGAGTCG 
GCACAATCAG AGAGTCTGGC TAAAACTGCT GGAGACGGTC CCTCTACATC CTCTGATTCC 
ACCACAGCTC CTCAGGAGTT CAGATGTCCT CCTCAGGCCA GTCACCTGAT CTGCACCTGC 
TGTCTGCAGC CCATGCCGGA CCGCCGCTTC GAGCACCTTC CCCCTCAGGT GTCCCCGCAG 
CACTGTTTGG TGTGCCAGAA ACCGTTCTGT CACGTGTACT GGGGCTGCCC GCGGATCGGC 
TGTCATGGCT GTTTAGCACG TTTCAGCGAA CTAAACCTCA ATGATAAATG TCTGGATGGA 
GTTTTTAATG GCAATCAATA CGAGTCGGAG GTCCTTCAGA ATTATCTGAG CTGTCGAGGG 
ATGAGCTGGA GGCATTTGCT GCAGGACTCG CTGCAGGCTC TACAGCAGGG GCTCTACCAT 
CTCTCTGATT ATAGAATCAC TGCCAACTCT TTTCTGTGCT ACTGCTGTGG ACTTCGCACT 
TTTCGAGAGC TCGCATACAA ATACAGAGAG CGTATTCCTC CATCAGAGCT TCCAGATGCT 
GTGACCAACA GACCGAACTG TTACTGGGGA CGCAACTGTC GAACTCAAGT GAAAGCACAT 
CATGCACTGA AGTTCAACCA CATATGTGAG CAGACCCGTT TTAAGAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

chfr ( XM_021476266.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021331941.1 Danio rerio checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase (chfr), transcript variant X2, mRNA.

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CloneID	ODa10198
Clone ID Related Accession (Same CDS sequence)	XM_017356139.1 , XM_017356139.2 , XM_021476266.1
Accession Version	XM_021476266.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1911bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase CHFR isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAAATC AGGACAGCGA TCAAGCCTGG GGGAAACTGG TGAAAGTGGA CGCATCTCCA 
GGTTCGGAGA TAGTGCTTAT AAATAGTGAA TGCACCGTTG GGAGAAAGAA AGATTGTGAC 
CTCTCATTCC CAGCTAATAA ACTGGTCTCA GGAAATCATT GCAAAATCAC TCATGATCAA 
AACTCAGGAA AGGTGTGGCT GGAAGACATG AGCACCAACG GAACTGTGAT CAACATGTCC 
AAAGTAGTGA AGAAACAGAC GCACTTACTG CAGAACGGTG ATGTCATTTA CTTTGTGTAC 
AGGAAGAACG AGCCAGAGCA AAACATTGCT TATGTTTATC AGTCCATCAC GCCACAGGAA 
AGTGCCTCAC ATGATGTTGA AGATGCAGGG AGAGAAGAAG ATTCTGATCT GACAGAAACG 
GAGAGTGAAC CAGCACCAGT AGAGCCTGTT ATCGTGAAGC CTTTACCTCA GTCTGGACAT 
GAGGATCCTC AGCCATCTAC CTCCAGCTCT TCCTTACACT TCTACAACAT GCCATTGTCT 
ACCTGCTCTG ATGTGTCTGC AAGAAAGAAT CCAGTCAGTT CTTCAGCTGT TTGTAAAGGA 
GACTCTACTT CATCAGGAAG TCCCGCTCAA ACCCGGCTGA AGTGGACCTG CTGGACTGAT 
GGAGAGCCGG AGGAGGAAAT GCAGAGGAAA AGAAGAAAAA CTGACAGAGA TGATCCAGGG 
TTTGGATCTG CTCACAGTGA TGCTTCTGCT GACATTCCCC TCAGAGGAGC AAGTGGAAAA 
GAAAAAACTG AAGGAGCCAC AACAGACAAG ATGGAGGAAT CCCTCACATG CATCATCTGT 
CAGGACCTGC TATATGACTG CATCAGTGTT CAGCCTTGCA TGCATACGTT TTGTGCGGCC 
TGTTATTCTG GCTGGATGGA GCGTTCCTCT TTCTGTCCCA CCTGTCGATG TCCTGTGGAG 
AGGATCCGCA AAAACCACAT CCTCAACAAC CTGGTGGAGG CTTACCTTCT CCAGCACCCG 
GAGAAGTGTC GGACTGAAGA TGATTTGCGC AGTATGGATG CCAGAAACAA GATCACACAG 
GACATGCTGC AGCCGAAGGT CGAGCGATCG TTCTCAGACG AGGAGGCCAG TTCAGACTAT 
CTGTTTGAAC TCTCAGACAA TGACAGCGAC ATTTCTGACA TGAGCCAACC CTACATGATG 
TGTCGTCAGT GTCCGGGTTA TCGAAAGGAA CTCAGCTCGG CCCTGTGGAT CTGCGAGTCG 
GCACAATCAG AGAGTCTGGC TAAAACTGCT GGAGACGGTC CCTCTACATC CTCTGATTCC 
ACCACAGCTC CTCAGGAGTT CAGATGTCCT CCTCAGGCCA GTCACCTGAT CTGCACCTGC 
TGTCTGCAGC CCATGCCGGA CCGCCGCTTC GAGCACCTTC CCCCTCAGGT GTCCCCGCAG 
CACTGTTTGG TGTGCCAGAA ACCGTTCTGT CACGTGTACT GGGGCTGCCC GCGGATCGGC 
TGTCATGGCT GTTTAGCACG TTTCAGCGAA CTAAACCTCA ATGATAAATG TCTGGATGGA 
GTTTTTAATG GCAATCAATA CGAGTCGGAG GTCCTTCAGA ATTATCTGAG CTGTCGAGGG 
ATGAGCTGGA GGCATTTGCT GCAGGACTCG CTGCAGGCTC TACAGCAGGG GCTCTACCAT 
CTCTCTGATT ATAGAATCAC TGCCAACTCT TTTCTGTGCT ACTGCTGTGG ACTTCGCACT 
TTTCGAGAGC TCGCATACAA ATACAGAGAG CGTATTCCTC CATCAGAGCT TCCAGATGCT 
GTGACCAACA GACCGAACTG TTACTGGGGA CGCAACTGTC GAACTCAAGT GAAAGCACAT 
CATGCACTGA AGTTCAACCA CATATGTGAG CAGACCCGTT TTAAGAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021331941.1
CDS	176..2086
Translation	MTNQDSDQAWGKLVKVDASPGSEIVLINSECTVGRKKDCDLSFPANKLVSGNHCKITHDQNSGKVWLEDMSTNGTVINMSKVVKKQTHLLQNGDVIYFVYRKNEPEQNIAYVYQSITPQESASHDVEDAGREEDSDLTETESEPAPVEPVIVKPLPQSGHEDPQPSTSSSSLHFYNMPLSTCSDVSARKNPVSSSAVCKGDSTSSGSPAQTRLKWTCWTDGEPEEEMQRKRRKTDRDDPGFGSAHSDASADIPLRGASGKEKTEGATTDKMEESLTCIICQDLLYDCISVQPCMHTFCAACYSGWMERSSFCPTCRCPVERIRKNHILNNLVEAYLLQHPEKCRTEDDLRSMDARNKITQDMLQPKVERSFSDEEASSDYLFELSDNDSDISDMSQPYMMCRQCPGYRKELSSALWICESAQSESLAKTAGDGPSTSSDSTTAPQEFRCPPQASHLICTCCLQPMPDRRFEHLPPQVSPQHCLVCQKPFCHVYWGCPRIGCHGCLARFSELNLNDKCLDGVFNGNQYESEVLQNYLSCRGMSWRHLLQDSLQALQQGLYHLSDYRITANSFLCYCCGLRTFRELAYKYRERIPPSELPDAVTNRPNCYWGRNCRTQVKAHHALKFNHICEQTRFKN

Target ORF information:

RefSeq Version	XM_021476266.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase (chfr), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476266.1

ORF Insert Sequence:

ATGACAAATC AGGACAGCGA TCAAGCCTGG GGGAAACTGG TGAAAGTGGA CGCATCTCCA 
GGTTCGGAGA TAGTGCTTAT AAATAGTGAA TGCACCGTTG GGAGAAAGAA AGATTGTGAC 
CTCTCATTCC CAGCTAATAA ACTGGTCTCA GGAAATCATT GCAAAATCAC TCATGATCAA 
AACTCAGGAA AGGTGTGGCT GGAAGACATG AGCACCAACG GAACTGTGAT CAACATGTCC 
AAAGTAGTGA AGAAACAGAC GCACTTACTG CAGAACGGTG ATGTCATTTA CTTTGTGTAC 
AGGAAGAACG AGCCAGAGCA AAACATTGCT TATGTTTATC AGTCCATCAC GCCACAGGAA 
AGTGCCTCAC ATGATGTTGA AGATGCAGGG AGAGAAGAAG ATTCTGATCT GACAGAAACG 
GAGAGTGAAC CAGCACCAGT AGAGCCTGTT ATCGTGAAGC CTTTACCTCA GTCTGGACAT 
GAGGATCCTC AGCCATCTAC CTCCAGCTCT TCCTTACACT TCTACAACAT GCCATTGTCT 
ACCTGCTCTG ATGTGTCTGC AAGAAAGAAT CCAGTCAGTT CTTCAGCTGT TTGTAAAGGA 
GACTCTACTT CATCAGGAAG TCCCGCTCAA ACCCGGCTGA AGTGGACCTG CTGGACTGAT 
GGAGAGCCGG AGGAGGAAAT GCAGAGGAAA AGAAGAAAAA CTGACAGAGA TGATCCAGGG 
TTTGGATCTG CTCACAGTGA TGCTTCTGCT GACATTCCCC TCAGAGGAGC AAGTGGAAAA 
GAAAAAACTG AAGGAGCCAC AACAGACAAG ATGGAGGAAT CCCTCACATG CATCATCTGT 
CAGGACCTGC TATATGACTG CATCAGTGTT CAGCCTTGCA TGCATACGTT TTGTGCGGCC 
TGTTATTCTG GCTGGATGGA GCGTTCCTCT TTCTGTCCCA CCTGTCGATG TCCTGTGGAG 
AGGATCCGCA AAAACCACAT CCTCAACAAC CTGGTGGAGG CTTACCTTCT CCAGCACCCG 
GAGAAGTGTC GGACTGAAGA TGATTTGCGC AGTATGGATG CCAGAAACAA GATCACACAG 
GACATGCTGC AGCCGAAGGT CGAGCGATCG TTCTCAGACG AGGAGGCCAG TTCAGACTAT 
CTGTTTGAAC TCTCAGACAA TGACAGCGAC ATTTCTGACA TGAGCCAACC CTACATGATG 
TGTCGTCAGT GTCCGGGTTA TCGAAAGGAA CTCAGCTCGG CCCTGTGGAT CTGCGAGTCG 
GCACAATCAG AGAGTCTGGC TAAAACTGCT GGAGACGGTC CCTCTACATC CTCTGATTCC 
ACCACAGCTC CTCAGGAGTT CAGATGTCCT CCTCAGGCCA GTCACCTGAT CTGCACCTGC 
TGTCTGCAGC CCATGCCGGA CCGCCGCTTC GAGCACCTTC CCCCTCAGGT GTCCCCGCAG 
CACTGTTTGG TGTGCCAGAA ACCGTTCTGT CACGTGTACT GGGGCTGCCC GCGGATCGGC 
TGTCATGGCT GTTTAGCACG TTTCAGCGAA CTAAACCTCA ATGATAAATG TCTGGATGGA 
GTTTTTAATG GCAATCAATA CGAGTCGGAG GTCCTTCAGA ATTATCTGAG CTGTCGAGGG 
ATGAGCTGGA GGCATTTGCT GCAGGACTCG CTGCAGGCTC TACAGCAGGG GCTCTACCAT 
CTCTCTGATT ATAGAATCAC TGCCAACTCT TTTCTGTGCT ACTGCTGTGG ACTTCGCACT 
TTTCGAGAGC TCGCATACAA ATACAGAGAG CGTATTCCTC CATCAGAGCT TCCAGATGCT 
GTGACCAACA GACCGAACTG TTACTGGGGA CGCAACTGTC GAACTCAAGT GAAAGCACAT 
CATGCACTGA AGTTCAACCA CATATGTGAG CAGACCCGTT TTAAGAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

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chfr cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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