CHFR cDNA ORF clone, Bos taurus(Cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	CHFR
Entrez Gene ID	614816
Full Name	checkpoint with forkhead and ring finger domains
General protein information	Preferred Names checkpoint with forkhead and ring finger domains Names E3 ubiquitin-protein ligase CHFR
Gene Type	protein-coding
Organism	Bos taurus(Cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005217679.4	XP_005217736.2	E3 ubiquitin-protein ligase CHFR isoform X1
NM_001205474.3	NP_001192403.2	E3 ubiquitin-protein ligase CHFR
NM_001205474.2	NP_001192403.1	E3 ubiquitin-protein ligase CHFR
XM_024977446.1	XP_024833214.1	E3 ubiquitin-protein ligase CHFR isoform X2
XM_005217679.3	XP_005217736.1	E3 ubiquitin-protein ligase CHFR isoform X1
NM_001205474.1	NP_001192403.1	E3 ubiquitin-protein ligase CHFR

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Chfr	NP_001009258.1
Xenopus tropicalis (tropical clawed frog)	chfr	NP_989378.1
	CHFR	XP_001083796.2
Oryza sativa (rice)	Os11g0175500	NP_001065879.2
Homo sapiens (human)	CHFR	NP_001154816.1
Pan troglodytes (chimpanzee)	CHFR	XP_003314094.2
Bos taurus (cattle)	CHFR	NP_001192403.1
Danio rerio (zebrafish)	chfr	NP_001093485.1
Canis lupus familiaris (dog)	CHFR	XP_534631.3
Arabidopsis thaliana (thale cress)	AT1G47570	NP_001077687.1
Mus musculus (house mouse)	Chfr	NP_766305.2
Gallus gallus (chicken)	CHFR	XP_004945549.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CHFR gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CHFR cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa27764	NM_001205474.2 Latest version!	Bos taurus checkpoint with forkhead and ring finger domains (CHFR), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa36751	NM_001205474.1 Latest version!	Bos taurus checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase (CHFR), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa27764	XM_005217679.3 Latest version!	Bos taurus checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase (CHFR), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa224391	NM_001205474.3 Latest version!	Bos taurus checkpoint with forkhead and ring finger domains (CHFR), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OBa224391	XM_005217679.4 Latest version!	Bos taurus checkpoint with forkhead and ring finger domains (CHFR), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OBa224392	XM_024977446.1 Latest version!	Bos taurus checkpoint with forkhead and ring finger domains (CHFR), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CHFR ( NM_001205474.2 ) cDNA ORF clone, Bos taurus(Cattle) -> NP_001192403.1 Bos taurus checkpoint with forkhead and ring finger domains (CHFR), mRNA.

Price & Availability↑

CloneID	OBa27764
Clone ID Related Accession (Same CDS sequence)	NM_001205474.2 , XM_005217679.3
Accession Version	NM_001205474.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1992bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-22
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase CHFR
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DAAA02044944.1. On Jun 30, 2017 this sequence version replaced NM_001205474.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN02822088, SAMN02822089 [ECO:0000348] ##Evidence-Data-END##

ATGGAGCGGC CGGGGGAAGG CGAGCAGCCG CCCCAGCAGC AGCCGTGGGG AAGGCTTCTT 
CGCCTGGGCG CCGAGGAGGG CGAGCCGCAC GTCCTCCTGA GGAAACGGGA GTGGACCATC 
GGGCGAAGAA GAGGTTGTGA CCTTTCATTC CCTGGCAATA AATTGGTCTC TGGAGATCAC 
TGTAAAATTA CAGTGGATGA AAAATCGGGT CAGGTATCAC TGGAAGATAC CAGTGCCAAT 
GGAACTGTCA TTAACAAGCT GAAGGTTGTT AAGAAGCAAA CTTGCCCTTT ACAGACTGGG 
GATGTCATCT ACTTGGTGTA CAGGAAGAAT GAGCCAGAAC ACAACGTGGC ATACCTCTAC 
GAATCTCTAA ATGAAAAGCA AGGTGTAACA CAAGACTCCT TTGAAGCTAA TAAAGAAAAT 
GTGTTCCACG TGACCAAAGA TACCTCAGGT GCAGGGCGAG GTGACGATCC TCAGGATCCC 
CAGGTCCTGC TGTCGTCGCC CGCCACTCAG GTGTGCTTTG AGGAACCACA GCCATCGACG 
TCCACATCGG ACCTCTTCCC CACGGCCTCT ACCTCTTCCA TGGAGCCCAC CTCCGCAGGC 
CGAGGGCCTC CCTCCAGCTC CAGCTCCAGA GGTACAGACA TCTCTCCAAA GGGGTGTGGT 
CCATCTGTGA CAAGTGATGA AATCTCCAGC TTCCCTTTAA CTCTCCCAGA CAGAGAGGGG 
GCTTCCTTTT CTCTGTCGGA ACCCCAGGAT CAGGAGGATT TGGAGCCTGT TAAGAAGAGA 
GTAAAAGCAG TTGGGGAGCC AGACCTTAAC CTGCCCATAC TGGTCACAGA CCCAGGCAGA 
GACCCTCCCG CTGCCCTTGA CGTCAGAGCC GAGGCCATGA AGCCAGACAA GATGGAGGAG 
ACGCTCACGT GCATCATCTG CCAGGACCTG CTGCACGACT GTGTGAGCCT GCAGCCCTGT 
ATGCACACCT TCTGCGCTGC CTGCTACTCG GGCTGGATGG AGCGGTCTGC CCTGTGCCCC 
ACCTGCCGCT GTCCGGTTGA GCGCATCTGT AAAAACCACA TCCTCAACAA CCTGGTGGAG 
GCGTACCTGC TGCAGCACCC GGACAAGAGG CGCAGCGAGG AGGATCTGCG CAGCATGGCC 
GCCAGGAACA AGATCACACA GGACATGCTG CAGCCCAAGG TCAGGAGGGC CTTCTCCGAC 
GAGGAGGGCA GCTCTGAGGA CCTGCTGGAG CTGTCGGACG TGGACAGTGA GTCCTCGGAC 
GTTAGCCAGC CGTCCATCGT GTGCAGACAG TGCCCTGAGT ACCGCAGGCA GGCGGGGCAG 
CCCCTTCCCT ACCCAGGGCC CGGCAGCGAG CCAGGGGCAC CGCAGGTCCC CGGGGATGCA 
CCGTCCACGT CCGCCAGTGT CACGGCAGCC CAGGACTACG TGTGCGCCCT GCAAGGAAGC 
CACGCCATAT GCACCTGCTG CTTCCAGCCC ATGCCGGACC GGAGAGCGGA GCGTGAGCGG 
GACCCCCGCA TCGCCCCCCA GCAGTGTGCC ATCTGCCTGC AGCCCTTCTG CCACCTATAC 
TGGGGCTGTG CCCGGACTGG CTGCCTCGGC TGCCTGGCCC CGTTCTGCGA GCTCAACCTG 
GGTGACCGGT GTCTGGACGG GGTGCTGAGC AACAACAACT ATGAGTCGGA CGTCCTGAAG 
AATTACCTGG CCACCAGGGG TTTGACTTGG AAAAACATGT TGACTGAGAG CCTCGGGGCT 
CTGCAGAGGG GAGTATTCCT GCTGTCTGAT TACAGAATCA CCGGGAACAC CGTGCTGTGC 
TACTGCTGTG GCCTGCGGAG CTTCCGCGAG CTCACCTATC AGTATCGGCA GAACATTCCT 
GCTTCTGAGC TACCAGCGGC CGTCACGTCC CGTCCTGATT GCTACTGGGG CCGCAACTGC 
CGCACTCAGG TGAAGGCCCA CCACGCAATG AAATTCAATC ACATCTGTGA ACAGACGAGG 
TTCAAGAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001192403.1
CDS	1..1992
Translation	MERPGEGEQPPQQQPWGRLLRLGAEEGEPHVLLRKREWTIGRRRGCDLSFPGNKLVSGDHCKITVDEKSGQVSLEDTSANGTVINKLKVVKKQTCPLQTGDVIYLVYRKNEPEHNVAYLYESLNEKQGVTQDSFEANKENVFHVTKDTSGAGRGDDPQDPQVLLSSPATQVCFEEPQPSTSTSDLFPTASTSSMEPTSAGRGPPSSSSSRGTDISPKGCGPSVTSDEISSFPLTLPDREGASFSLSEPQDQEDLEPVKKRVKAVGEPDLNLPILVTDPGRDPPAALDVRAEAMKPDKMEETLTCIICQDLLHDCVSLQPCMHTFCAACYSGWMERSALCPTCRCPVERICKNHILNNLVEAYLLQHPDKRRSEEDLRSMAARNKITQDMLQPKVRRAFSDEEGSSEDLLELSDVDSESSDVSQPSIVCRQCPEYRRQAGQPLPYPGPGSEPGAPQVPGDAPSTSASVTAAQDYVCALQGSHAICTCCFQPMPDRRAERERDPRIAPQQCAICLQPFCHLYWGCARTGCLGCLAPFCELNLGDRCLDGVLSNNNYESDVLKNYLATRGLTWKNMLTESLGALQRGVFLLSDYRITGNTVLCYCCGLRSFRELTYQYRQNIPASELPAAVTSRPDCYWGRNCRTQVKAHHAMKFNHICEQTRFKN

Target ORF information:

RefSeq Version	NM_001205474.2
Organism	Bos taurus(cattle)
Definition	Bos taurus checkpoint with forkhead and ring finger domains (CHFR), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001205474.2

ORF Insert Sequence:

ATGGAGCGGC CGGGGGAAGG CGAGCAGCCG CCCCAGCAGC AGCCGTGGGG AAGGCTTCTT 
CGCCTGGGCG CCGAGGAGGG CGAGCCGCAC GTCCTCCTGA GGAAACGGGA GTGGACCATC 
GGGCGAAGAA GAGGTTGTGA CCTTTCATTC CCTGGCAATA AATTGGTCTC TGGAGATCAC 
TGTAAAATTA CAGTGGATGA AAAATCGGGT CAGGTATCAC TGGAAGATAC CAGTGCCAAT 
GGAACTGTCA TTAACAAGCT GAAGGTTGTT AAGAAGCAAA CTTGCCCTTT ACAGACTGGG 
GATGTCATCT ACTTGGTGTA CAGGAAGAAT GAGCCAGAAC ACAACGTGGC ATACCTCTAC 
GAATCTCTAA ATGAAAAGCA AGGTGTAACA CAAGACTCCT TTGAAGCTAA TAAAGAAAAT 
GTGTTCCACG TGACCAAAGA TACCTCAGGT GCAGGGCGAG GTGACGATCC TCAGGATCCC 
CAGGTCCTGC TGTCGTCGCC CGCCACTCAG GTGTGCTTTG AGGAACCACA GCCATCGACG 
TCCACATCGG ACCTCTTCCC CACGGCCTCT ACCTCTTCCA TGGAGCCCAC CTCCGCAGGC 
CGAGGGCCTC CCTCCAGCTC CAGCTCCAGA GGTACAGACA TCTCTCCAAA GGGGTGTGGT 
CCATCTGTGA CAAGTGATGA AATCTCCAGC TTCCCTTTAA CTCTCCCAGA CAGAGAGGGG 
GCTTCCTTTT CTCTGTCGGA ACCCCAGGAT CAGGAGGATT TGGAGCCTGT TAAGAAGAGA 
GTAAAAGCAG TTGGGGAGCC AGACCTTAAC CTGCCCATAC TGGTCACAGA CCCAGGCAGA 
GACCCTCCCG CTGCCCTTGA CGTCAGAGCC GAGGCCATGA AGCCAGACAA GATGGAGGAG 
ACGCTCACGT GCATCATCTG CCAGGACCTG CTGCACGACT GTGTGAGCCT GCAGCCCTGT 
ATGCACACCT TCTGCGCTGC CTGCTACTCG GGCTGGATGG AGCGGTCTGC CCTGTGCCCC 
ACCTGCCGCT GTCCGGTTGA GCGCATCTGT AAAAACCACA TCCTCAACAA CCTGGTGGAG 
GCGTACCTGC TGCAGCACCC GGACAAGAGG CGCAGCGAGG AGGATCTGCG CAGCATGGCC 
GCCAGGAACA AGATCACACA GGACATGCTG CAGCCCAAGG TCAGGAGGGC CTTCTCCGAC 
GAGGAGGGCA GCTCTGAGGA CCTGCTGGAG CTGTCGGACG TGGACAGTGA GTCCTCGGAC 
GTTAGCCAGC CGTCCATCGT GTGCAGACAG TGCCCTGAGT ACCGCAGGCA GGCGGGGCAG 
CCCCTTCCCT ACCCAGGGCC CGGCAGCGAG CCAGGGGCAC CGCAGGTCCC CGGGGATGCA 
CCGTCCACGT CCGCCAGTGT CACGGCAGCC CAGGACTACG TGTGCGCCCT GCAAGGAAGC 
CACGCCATAT GCACCTGCTG CTTCCAGCCC ATGCCGGACC GGAGAGCGGA GCGTGAGCGG 
GACCCCCGCA TCGCCCCCCA GCAGTGTGCC ATCTGCCTGC AGCCCTTCTG CCACCTATAC 
TGGGGCTGTG CCCGGACTGG CTGCCTCGGC TGCCTGGCCC CGTTCTGCGA GCTCAACCTG 
GGTGACCGGT GTCTGGACGG GGTGCTGAGC AACAACAACT ATGAGTCGGA CGTCCTGAAG 
AATTACCTGG CCACCAGGGG TTTGACTTGG AAAAACATGT TGACTGAGAG CCTCGGGGCT 
CTGCAGAGGG GAGTATTCCT GCTGTCTGAT TACAGAATCA CCGGGAACAC CGTGCTGTGC 
TACTGCTGTG GCCTGCGGAG CTTCCGCGAG CTCACCTATC AGTATCGGCA GAACATTCCT 
GCTTCTGAGC TACCAGCGGC CGTCACGTCC CGTCCTGATT GCTACTGGGG CCGCAACTGC 
CGCACTCAGG TGAAGGCCCA CCACGCAATG AAATTCAATC ACATCTGTGA ACAGACGAGG 
TTCAAGAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHFR ( NM_001205474.1 ) cDNA ORF clone, Bos taurus(Cattle) -> NP_001192403.1 Bos taurus checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase (CHFR), mRNA.

Price & Availability↑

CloneID	OBa36751
Clone ID Related Accession (Same CDS sequence)	NM_001205474.1
Accession Version	NM_001205474.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1992bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-03
Organism	Bos taurus(Cattle)
Product	E3 ubiquitin-protein ligase CHFR
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAFC03091943.1. On Apr 20, 2011 this sequence version replaced XM_001788064.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00000728, SAMN01093727 [ECO:0000348] ##Evidence-Data-END##

ATGGAGCGGC CGGGGGAAGG CGAGCAGCCG CCCCAGCAGC AGCCGTGGGG AAGGCTTCTT 
CGCCTGGGCG CCGAGGAGGG CGAGCCGCAC GTCCTCCTGA GGAAACGGGA GTGGACCATC 
GGGCGAAGAA GAGGTTGTGA CCTTTCATTC CCTGGCAATA AATTGGTCTC TGGAGATCAC 
TGTAAAATTA CAGTGGATGA AAAATCGGGT CAGGTATCAC TGGAAGATAC CAGTGCCAAT 
GGAACTGTCA TTAACAAGCT GAAGGTTGTT AAGAAGCAAA CTTGCCCTTT ACAGACTGGG 
GATGTCATCT ACTTGGTGTA CAGGAAGAAT GAGCCAGAAC ACAACGTGGC ATACCTCTAC 
GAATCTCTAA ATGAAAAGCA AGGTGTAACA CAAGACTCCT TTGAAGCTAA TAAAGAAAAT 
GTGTTCCACG TGACCAAAGA TACCTCAGGT GCAGGGCGAG GTGACGATCC TCAGGATCCC 
CAGGTCCTGC TGTCGTCGCC CGCCACTCAG GTGTGCTTTG AGGAACCACA GCCATCGACG 
TCCACATCGG ACCTCTTCCC CACGGCCTCT ACCTCTTCCA TGGAGCCCAC CTCCGCAGGC 
CGAGGGCCTC CCTCCAGCTC CAGCTCCAGA GGTACAGACA TCTCTCCAAA GGGGTGTGGT 
CCATCTGTGA CAAGTGATGA AATCTCCAGC TTCCCTTTAA CTCTCCCAGA CAGAGAGGGG 
GCTTCCTTTT CTCTGTCGGA ACCCCAGGAT CAGGAGGATT TGGAGCCTGT TAAGAAGAGA 
GTAAAAGCAG TTGGGGAGCC AGACCTTAAC CTGCCCATAC TGGTCACAGA CCCAGGCAGA 
GACCCTCCCG CTGCCCTTGA CGTCAGAGCC GAGGCCATGA AGCCAGACAA GATGGAGGAG 
ACGCTCACGT GCATCATCTG CCAGGACCTG CTGCACGACT GTGTGAGCCT GCAGCCCTGT 
ATGCACACCT TCTGCGCTGC CTGCTACTCG GGCTGGATGG AGCGGTCTGC CCTGTGCCCC 
ACCTGCCGCT GTCCGGTTGA GCGCATCTGT AAAAACCACA TCCTCAACAA CCTGGTGGAG 
GCGTACCTGC TGCAGCACCC GGACAAGAGG CGCAGCGAGG AGGATCTGCG CAGCATGGCC 
GCCAGGAACA AGATCACACA GGACATGCTG CAGCCCAAGG TCAGGAGGGC CTTCTCCGAC 
GAGGAGGGCA GCTCTGAGGA CCTGCTGGAG CTGTCGGACG TGGACAGTGA GTCCTCGGAC 
GTTAGCCAGC CGTCCATCGT GTGCAGACAG TGCCCTGAGT ACCGCAGGCA GGCGGGGCAG 
CCCCTTCCCT ACCCAGGGCC CGGCAGCGAG CCAGGGGCAC CGCAGGTCCC CGGGGATGCA 
CCGTCCACGT CCGCCAGTGT CACGGCAGCC CAGGACTACG TGTGCGCCCT TCAAGGAAGC 
CACGCCATAT GCACCTGCTG CTTCCAGCCC ATGCCGGACC GGAGAGCGGA GCGTGAGCGG 
GACCCCCGCA TCGCCCCCCA GCAGTGTGCC ATCTGCCTGC AGCCCTTCTG CCACCTATAC 
TGGGGCTGTG CCCGGACTGG CTGCCTCGGC TGCCTGGCCC CGTTCTGCGA GCTCAACCTG 
GGTGACCGGT GTCTGGACGG GGTGCTGAGC AACAACAACT ATGAGTCGGA CGTCCTGAAG 
AATTACCTGG CCACCAGGGG TTTGACTTGG AAAAACATGT TGACTGAGAG CCTCGGGGCT 
CTGCAGAGGG GAGTATTCCT GCTGTCTGAT TACAGAATCA CCGGGAACAC CGTGCTGTGC 
TACTGCTGTG GCCTGCGGAG CTTCCGCGAG CTCACCTACC AGTATCGGCA GAACATTCCT 
GCTTCTGAGC TACCAGCGGC CGTCACGTCC CGTCCTGATT GCTACTGGGG CCGCAACTGC 
CGCACTCAGG TGAAGGCCCA CCACGCAATG AAATTCAATC ACATCTGTGA ACAGACGAGG 
TTCAAGAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001192403.1
CDS	1..1992
Translation	MERPGEGEQPPQQQPWGRLLRLGAEEGEPHVLLRKREWTIGRRRGCDLSFPGNKLVSGDHCKITVDEKSGQVSLEDTSANGTVINKLKVVKKQTCPLQTGDVIYLVYRKNEPEHNVAYLYESLNEKQGVTQDSFEANKENVFHVTKDTSGAGRGDDPQDPQVLLSSPATQVCFEEPQPSTSTSDLFPTASTSSMEPTSAGRGPPSSSSSRGTDISPKGCGPSVTSDEISSFPLTLPDREGASFSLSEPQDQEDLEPVKKRVKAVGEPDLNLPILVTDPGRDPPAALDVRAEAMKPDKMEETLTCIICQDLLHDCVSLQPCMHTFCAACYSGWMERSALCPTCRCPVERICKNHILNNLVEAYLLQHPDKRRSEEDLRSMAARNKITQDMLQPKVRRAFSDEEGSSEDLLELSDVDSESSDVSQPSIVCRQCPEYRRQAGQPLPYPGPGSEPGAPQVPGDAPSTSASVTAAQDYVCALQGSHAICTCCFQPMPDRRAERERDPRIAPQQCAICLQPFCHLYWGCARTGCLGCLAPFCELNLGDRCLDGVLSNNNYESDVLKNYLATRGLTWKNMLTESLGALQRGVFLLSDYRITGNTVLCYCCGLRSFRELTYQYRQNIPASELPAAVTSRPDCYWGRNCRTQVKAHHAMKFNHICEQTRFKN

Target ORF information:

RefSeq Version	NM_001205474.1
Organism	Bos taurus(Cattle)
Definition	Bos taurus checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase (CHFR), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001205474.1

ORF Insert Sequence:

ATGGAGCGGC CGGGGGAAGG CGAGCAGCCG CCCCAGCAGC AGCCGTGGGG AAGGCTTCTT 
CGCCTGGGCG CCGAGGAGGG CGAGCCGCAC GTCCTCCTGA GGAAACGGGA GTGGACCATC 
GGGCGAAGAA GAGGTTGTGA CCTTTCATTC CCTGGCAATA AATTGGTCTC TGGAGATCAC 
TGTAAAATTA CAGTGGATGA AAAATCGGGT CAGGTATCAC TGGAAGATAC CAGTGCCAAT 
GGAACTGTCA TTAACAAGCT GAAGGTTGTT AAGAAGCAAA CTTGCCCTTT ACAGACTGGG 
GATGTCATCT ACTTGGTGTA CAGGAAGAAT GAGCCAGAAC ACAACGTGGC ATACCTCTAC 
GAATCTCTAA ATGAAAAGCA AGGTGTAACA CAAGACTCCT TTGAAGCTAA TAAAGAAAAT 
GTGTTCCACG TGACCAAAGA TACCTCAGGT GCAGGGCGAG GTGACGATCC TCAGGATCCC 
CAGGTCCTGC TGTCGTCGCC CGCCACTCAG GTGTGCTTTG AGGAACCACA GCCATCGACG 
TCCACATCGG ACCTCTTCCC CACGGCCTCT ACCTCTTCCA TGGAGCCCAC CTCCGCAGGC 
CGAGGGCCTC CCTCCAGCTC CAGCTCCAGA GGTACAGACA TCTCTCCAAA GGGGTGTGGT 
CCATCTGTGA CAAGTGATGA AATCTCCAGC TTCCCTTTAA CTCTCCCAGA CAGAGAGGGG 
GCTTCCTTTT CTCTGTCGGA ACCCCAGGAT CAGGAGGATT TGGAGCCTGT TAAGAAGAGA 
GTAAAAGCAG TTGGGGAGCC AGACCTTAAC CTGCCCATAC TGGTCACAGA CCCAGGCAGA 
GACCCTCCCG CTGCCCTTGA CGTCAGAGCC GAGGCCATGA AGCCAGACAA GATGGAGGAG 
ACGCTCACGT GCATCATCTG CCAGGACCTG CTGCACGACT GTGTGAGCCT GCAGCCCTGT 
ATGCACACCT TCTGCGCTGC CTGCTACTCG GGCTGGATGG AGCGGTCTGC CCTGTGCCCC 
ACCTGCCGCT GTCCGGTTGA GCGCATCTGT AAAAACCACA TCCTCAACAA CCTGGTGGAG 
GCGTACCTGC TGCAGCACCC GGACAAGAGG CGCAGCGAGG AGGATCTGCG CAGCATGGCC 
GCCAGGAACA AGATCACACA GGACATGCTG CAGCCCAAGG TCAGGAGGGC CTTCTCCGAC 
GAGGAGGGCA GCTCTGAGGA CCTGCTGGAG CTGTCGGACG TGGACAGTGA GTCCTCGGAC 
GTTAGCCAGC CGTCCATCGT GTGCAGACAG TGCCCTGAGT ACCGCAGGCA GGCGGGGCAG 
CCCCTTCCCT ACCCAGGGCC CGGCAGCGAG CCAGGGGCAC CGCAGGTCCC CGGGGATGCA 
CCGTCCACGT CCGCCAGTGT CACGGCAGCC CAGGACTACG TGTGCGCCCT TCAAGGAAGC 
CACGCCATAT GCACCTGCTG CTTCCAGCCC ATGCCGGACC GGAGAGCGGA GCGTGAGCGG 
GACCCCCGCA TCGCCCCCCA GCAGTGTGCC ATCTGCCTGC AGCCCTTCTG CCACCTATAC 
TGGGGCTGTG CCCGGACTGG CTGCCTCGGC TGCCTGGCCC CGTTCTGCGA GCTCAACCTG 
GGTGACCGGT GTCTGGACGG GGTGCTGAGC AACAACAACT ATGAGTCGGA CGTCCTGAAG 
AATTACCTGG CCACCAGGGG TTTGACTTGG AAAAACATGT TGACTGAGAG CCTCGGGGCT 
CTGCAGAGGG GAGTATTCCT GCTGTCTGAT TACAGAATCA CCGGGAACAC CGTGCTGTGC 
TACTGCTGTG GCCTGCGGAG CTTCCGCGAG CTCACCTACC AGTATCGGCA GAACATTCCT 
GCTTCTGAGC TACCAGCGGC CGTCACGTCC CGTCCTGATT GCTACTGGGG CCGCAACTGC 
CGCACTCAGG TGAAGGCCCA CCACGCAATG AAATTCAATC ACATCTGTGA ACAGACGAGG 
TTCAAGAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHFR ( XM_005217679.3 ) cDNA ORF clone, Bos taurus(Cattle) -> XP_005217736.1 Bos taurus checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase (CHFR), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa27764
Clone ID Related Accession (Same CDS sequence)	NM_001205474.2 , XM_005217679.3
Accession Version	XM_005217679.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1992bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase CHFR isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104452.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005217679.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCGGC CGGGGGAAGG CGAGCAGCCG CCCCAGCAGC AGCCGTGGGG AAGGCTTCTT 
CGCCTGGGCG CCGAGGAGGG CGAGCCGCAC GTCCTCCTGA GGAAACGGGA GTGGACCATC 
GGGCGAAGAA GAGGTTGTGA CCTTTCATTC CCTGGCAATA AATTGGTCTC TGGAGATCAC 
TGTAAAATTA CAGTGGATGA AAAATCGGGT CAGGTATCAC TGGAAGATAC CAGTGCCAAT 
GGAACTGTCA TTAACAAGCT GAAGGTTGTT AAGAAGCAAA CTTGCCCTTT ACAGACTGGG 
GATGTCATCT ACTTGGTGTA CAGGAAGAAT GAGCCAGAAC ACAACGTGGC ATACCTCTAC 
GAATCTCTAA ATGAAAAGCA AGGTGTAACA CAAGACTCCT TTGAAGCTAA TAAAGAAAAT 
GTGTTCCACG TGACCAAAGA TACCTCAGGT GCAGGGCGAG GTGACGATCC TCAGGATCCC 
CAGGTCCTGC TGTCGTCGCC CGCCACTCAG GTGTGCTTTG AGGAACCACA GCCATCGACG 
TCCACATCGG ACCTCTTCCC CACGGCCTCT ACCTCTTCCA TGGAGCCCAC CTCCGCAGGC 
CGAGGGCCTC CCTCCAGCTC CAGCTCCAGA GGTACAGACA TCTCTCCAAA GGGGTGTGGT 
CCATCTGTGA CAAGTGATGA AATCTCCAGC TTCCCTTTAA CTCTCCCAGA CAGAGAGGGG 
GCTTCCTTTT CTCTGTCGGA ACCCCAGGAT CAGGAGGATT TGGAGCCTGT TAAGAAGAGA 
GTAAAAGCAG TTGGGGAGCC AGACCTTAAC CTGCCCATAC TGGTCACAGA CCCAGGCAGA 
GACCCTCCCG CTGCCCTTGA CGTCAGAGCC GAGGCCATGA AGCCAGACAA GATGGAGGAG 
ACGCTCACGT GCATCATCTG CCAGGACCTG CTGCACGACT GTGTGAGCCT GCAGCCCTGT 
ATGCACACCT TCTGCGCTGC CTGCTACTCG GGCTGGATGG AGCGGTCTGC CCTGTGCCCC 
ACCTGCCGCT GTCCGGTTGA GCGCATCTGT AAAAACCACA TCCTCAACAA CCTGGTGGAG 
GCGTACCTGC TGCAGCACCC GGACAAGAGG CGCAGCGAGG AGGATCTGCG CAGCATGGCC 
GCCAGGAACA AGATCACACA GGACATGCTG CAGCCCAAGG TCAGGAGGGC CTTCTCCGAC 
GAGGAGGGCA GCTCTGAGGA CCTGCTGGAG CTGTCGGACG TGGACAGTGA GTCCTCGGAC 
GTTAGCCAGC CGTCCATCGT GTGCAGACAG TGCCCTGAGT ACCGCAGGCA GGCGGGGCAG 
CCCCTTCCCT ACCCAGGGCC CGGCAGCGAG CCAGGGGCAC CGCAGGTCCC CGGGGATGCA 
CCGTCCACGT CCGCCAGTGT CACGGCAGCC CAGGACTACG TGTGCGCCCT GCAAGGAAGC 
CACGCCATAT GCACCTGCTG CTTCCAGCCC ATGCCGGACC GGAGAGCGGA GCGTGAGCGG 
GACCCCCGCA TCGCCCCCCA GCAGTGTGCC ATCTGCCTGC AGCCCTTCTG CCACCTATAC 
TGGGGCTGTG CCCGGACTGG CTGCCTCGGC TGCCTGGCCC CGTTCTGCGA GCTCAACCTG 
GGTGACCGGT GTCTGGACGG GGTGCTGAGC AACAACAACT ATGAGTCGGA CGTCCTGAAG 
AATTACCTGG CCACCAGGGG TTTGACTTGG AAAAACATGT TGACTGAGAG CCTCGGGGCT 
CTGCAGAGGG GAGTATTCCT GCTGTCTGAT TACAGAATCA CCGGGAACAC CGTGCTGTGC 
TACTGCTGTG GCCTGCGGAG CTTCCGCGAG CTCACCTATC AGTATCGGCA GAACATTCCT 
GCTTCTGAGC TACCAGCGGC CGTCACGTCC CGTCCTGATT GCTACTGGGG CCGCAACTGC 
CGCACTCAGG TGAAGGCCCA CCACGCAATG AAATTCAATC ACATCTGTGA ACAGACGAGG 
TTCAAGAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005217736.1
CDS	107..2098
Translation	MERPGEGEQPPQQQPWGRLLRLGAEEGEPHVLLRKREWTIGRRRGCDLSFPGNKLVSGDHCKITVDEKSGQVSLEDTSANGTVINKLKVVKKQTCPLQTGDVIYLVYRKNEPEHNVAYLYESLNEKQGVTQDSFEANKENVFHVTKDTSGAGRGDDPQDPQVLLSSPATQVCFEEPQPSTSTSDLFPTASTSSMEPTSAGRGPPSSSSSRGTDISPKGCGPSVTSDEISSFPLTLPDREGASFSLSEPQDQEDLEPVKKRVKAVGEPDLNLPILVTDPGRDPPAALDVRAEAMKPDKMEETLTCIICQDLLHDCVSLQPCMHTFCAACYSGWMERSALCPTCRCPVERICKNHILNNLVEAYLLQHPDKRRSEEDLRSMAARNKITQDMLQPKVRRAFSDEEGSSEDLLELSDVDSESSDVSQPSIVCRQCPEYRRQAGQPLPYPGPGSEPGAPQVPGDAPSTSASVTAAQDYVCALQGSHAICTCCFQPMPDRRAERERDPRIAPQQCAICLQPFCHLYWGCARTGCLGCLAPFCELNLGDRCLDGVLSNNNYESDVLKNYLATRGLTWKNMLTESLGALQRGVFLLSDYRITGNTVLCYCCGLRSFRELTYQYRQNIPASELPAAVTSRPDCYWGRNCRTQVKAHHAMKFNHICEQTRFKN

Target ORF information:

RefSeq Version	XM_005217679.3
Organism	Bos taurus(cattle)
Definition	Bos taurus checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase (CHFR), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005217679.3

ORF Insert Sequence:

ATGGAGCGGC CGGGGGAAGG CGAGCAGCCG CCCCAGCAGC AGCCGTGGGG AAGGCTTCTT 
CGCCTGGGCG CCGAGGAGGG CGAGCCGCAC GTCCTCCTGA GGAAACGGGA GTGGACCATC 
GGGCGAAGAA GAGGTTGTGA CCTTTCATTC CCTGGCAATA AATTGGTCTC TGGAGATCAC 
TGTAAAATTA CAGTGGATGA AAAATCGGGT CAGGTATCAC TGGAAGATAC CAGTGCCAAT 
GGAACTGTCA TTAACAAGCT GAAGGTTGTT AAGAAGCAAA CTTGCCCTTT ACAGACTGGG 
GATGTCATCT ACTTGGTGTA CAGGAAGAAT GAGCCAGAAC ACAACGTGGC ATACCTCTAC 
GAATCTCTAA ATGAAAAGCA AGGTGTAACA CAAGACTCCT TTGAAGCTAA TAAAGAAAAT 
GTGTTCCACG TGACCAAAGA TACCTCAGGT GCAGGGCGAG GTGACGATCC TCAGGATCCC 
CAGGTCCTGC TGTCGTCGCC CGCCACTCAG GTGTGCTTTG AGGAACCACA GCCATCGACG 
TCCACATCGG ACCTCTTCCC CACGGCCTCT ACCTCTTCCA TGGAGCCCAC CTCCGCAGGC 
CGAGGGCCTC CCTCCAGCTC CAGCTCCAGA GGTACAGACA TCTCTCCAAA GGGGTGTGGT 
CCATCTGTGA CAAGTGATGA AATCTCCAGC TTCCCTTTAA CTCTCCCAGA CAGAGAGGGG 
GCTTCCTTTT CTCTGTCGGA ACCCCAGGAT CAGGAGGATT TGGAGCCTGT TAAGAAGAGA 
GTAAAAGCAG TTGGGGAGCC AGACCTTAAC CTGCCCATAC TGGTCACAGA CCCAGGCAGA 
GACCCTCCCG CTGCCCTTGA CGTCAGAGCC GAGGCCATGA AGCCAGACAA GATGGAGGAG 
ACGCTCACGT GCATCATCTG CCAGGACCTG CTGCACGACT GTGTGAGCCT GCAGCCCTGT 
ATGCACACCT TCTGCGCTGC CTGCTACTCG GGCTGGATGG AGCGGTCTGC CCTGTGCCCC 
ACCTGCCGCT GTCCGGTTGA GCGCATCTGT AAAAACCACA TCCTCAACAA CCTGGTGGAG 
GCGTACCTGC TGCAGCACCC GGACAAGAGG CGCAGCGAGG AGGATCTGCG CAGCATGGCC 
GCCAGGAACA AGATCACACA GGACATGCTG CAGCCCAAGG TCAGGAGGGC CTTCTCCGAC 
GAGGAGGGCA GCTCTGAGGA CCTGCTGGAG CTGTCGGACG TGGACAGTGA GTCCTCGGAC 
GTTAGCCAGC CGTCCATCGT GTGCAGACAG TGCCCTGAGT ACCGCAGGCA GGCGGGGCAG 
CCCCTTCCCT ACCCAGGGCC CGGCAGCGAG CCAGGGGCAC CGCAGGTCCC CGGGGATGCA 
CCGTCCACGT CCGCCAGTGT CACGGCAGCC CAGGACTACG TGTGCGCCCT GCAAGGAAGC 
CACGCCATAT GCACCTGCTG CTTCCAGCCC ATGCCGGACC GGAGAGCGGA GCGTGAGCGG 
GACCCCCGCA TCGCCCCCCA GCAGTGTGCC ATCTGCCTGC AGCCCTTCTG CCACCTATAC 
TGGGGCTGTG CCCGGACTGG CTGCCTCGGC TGCCTGGCCC CGTTCTGCGA GCTCAACCTG 
GGTGACCGGT GTCTGGACGG GGTGCTGAGC AACAACAACT ATGAGTCGGA CGTCCTGAAG 
AATTACCTGG CCACCAGGGG TTTGACTTGG AAAAACATGT TGACTGAGAG CCTCGGGGCT 
CTGCAGAGGG GAGTATTCCT GCTGTCTGAT TACAGAATCA CCGGGAACAC CGTGCTGTGC 
TACTGCTGTG GCCTGCGGAG CTTCCGCGAG CTCACCTATC AGTATCGGCA GAACATTCCT 
GCTTCTGAGC TACCAGCGGC CGTCACGTCC CGTCCTGATT GCTACTGGGG CCGCAACTGC 
CGCACTCAGG TGAAGGCCCA CCACGCAATG AAATTCAATC ACATCTGTGA ACAGACGAGG 
TTCAAGAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHFR ( NM_001205474.3 ) cDNA ORF clone, Bos taurus(Cattle) -> NP_001192403.2 Bos taurus checkpoint with forkhead and ring finger domains (CHFR), mRNA.

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CloneID	OBa224391
Clone ID Related Accession (Same CDS sequence)	XM_005217679.4 , NM_001205474.3
Accession Version	NM_001205474.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1992bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-19
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase CHFR
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000017.1. On Dec 20, 2018 this sequence version replaced NM_001205474.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: GGVH01008198.1, GGUY01128769.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145414 [ECO:0000348] ##Evidence-Data-END##

ATGGAGCGGC CGGGGGAAGG CGAGCAGCCG CCCCAGCAGC AGCCGTGGGG AAGGCTTCTT 
CGCCTGGGCG CCGAGGAGGG CGAGCCGCAC GTCCTCCTGA GGAAACGGGA GTGGACCATC 
GGGCGAAGAA GAGGTTGTGA CCTTTCATTC CCTGGCAATA AATTGGTCTC TGGAGATCAC 
TGTAAAATTA CAGTGGATGA AAAATCGGGT CAGGTATCAC TGGAAGATAC CAGTGCCAAT 
GGAACTGTCA TTAACAAGCT GAAGGTTGTT AAGAAGCAAA CTTGCCCTTT ACAGACTGGG 
GATGTCATCT ACTTGGTGTA CAGGAAGAAT GAGCCAGAAC ACAACGTGGC ATACCTCTAC 
GAATCTCTAA ATGAAAAGCA AGGTGTAACA CAAGACTCCT TTGAAGCTAA TAAAGAAAAT 
GTGTTCCACG TGACCAAAGA TACCTCAGGT GCAGGGCGAG GTGACGATCC TCAGGATCCC 
CAGGTCCTGC TGTCGTCGCC CGCCACTCAG GTGTGCTTTG AGGAACCACA GCCATCGACG 
TCCACATCGG ACCTCTTCCC CACGGCCTCT ACCTCTTCCA TGGAGCCCAC CTCCGCAGGC 
CGAGGGCCTC CCTCCAGCTC CAGCTCCAGA GGTACAGACA TCTCTCCAAA GGGGTGTGGT 
CCATCTGTGA CAAGTGATGA AATCTCCAGC TTCCCTTTAA CTCTCCCAGA CAGAGAGGGG 
GCTTCCTTTT CTCTGTCGGA ACCCCAGGAT CAGGAGGATT TGGAGCCTGT TAAGAAGAGA 
GTAAAAGCAG TTGGGGAGCC AGACCTTAAC CTGCCCATAC TGGTCACAGA CCCAGGCAGA 
GACCCTCCCG CTGCCCTTGA CGTCAGAGCC GAGGCCATGA AGCCAGACAA GATGGAGGAG 
ACGCTCACGT GCATCATCTG CCAGGACCTG CTGCACGACT GTGTGAGCCT GCAGCCCTGT 
ATGCACACCT TCTGCGCTGC CTGCTACTCG GGCTGGATGG AGCGGTCTGC CCTGTGCCCC 
ACCTGCCGCT GTCCGGTTGA GCGCATCTGT AAAAACCACA TCCTCAACAA CCTGGTGGAG 
GCGTACCTGC TGCAGCACCC GGACAAGAGG CGCAGCGAGG AGGATCTGCG CAGCATGGCC 
GCCAGGAACA AGATCACACA GGACATGCTG CAGCCCAAGG TCAGGAGGGC CTTCTCCGAC 
GAGGAGGGCA GCTCTGAGGA CCTGCTGGAG CTGTCGGACG TGGACAGTGA GTCCTCGGAC 
GTTAGCCAGC CGTCCATCGT GTGCAGACAG TGCCCTGAGT ACCGCAGGCA GGCGGGGCAG 
CCCCTTCCCT ACCCAGGGCC CGGCAGCGAG CCAGGGGCAC CGCAGGTCCC CGGGGATGCA 
CCGTCCACGT CCGCCAGTGT CATGGCAGCC CAGGACTACG TGTGCGCCCT TCAAGGAAGC 
CACGCCATAT GCACCTGCTG CTTCCAGCCC ATGCCGGACC GGAGAGCGGA GCGTGAGCGG 
GACCCCCGCA TCGCCCCCCA GCAGTGTGCC ATCTGCCTGC AGCCCTTCTG CCACCTATAC 
TGGGGCTGTG CCCGGACTGG CTGCCTCGGC TGCCTGGCCC CGTTCTGCGA GCTCAACCTG 
GGTGACCGGT GTCTGGACGG GGTGCTGAGC AACAACAACT ATGAGTCGGA CGTCCTGAAG 
AATTACCTGG CCACCAGGGG TTTGACTTGG AAAAACATGT TGACTGAGAG CCTCGGGGCT 
CTGCAGAGGG GAGTATTCCT GCTGTCTGAT TACAGAATCA CCGGGAACAC CGTGCTGTGC 
TACTGCTGTG GCCTGCGGAG CTTCCGCGAG CTCACCTATC AGTATCGGCA GAACATTCCT 
GCTTCTGAGC TACCAGCGGC CGTCACGTCC CGTCCTGATT GCTACTGGGG CCGCAACTGC 
CGCACTCAGG TGAAGGCCCA CCACGCAATG AAATTCAATC ACATCTGTGA ACAGACGAGG 
TTCAAGAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001192403.2
CDS	1..1992
Translation	MERPGEGEQPPQQQPWGRLLRLGAEEGEPHVLLRKREWTIGRRRGCDLSFPGNKLVSGDHCKITVDEKSGQVSLEDTSANGTVINKLKVVKKQTCPLQTGDVIYLVYRKNEPEHNVAYLYESLNEKQGVTQDSFEANKENVFHVTKDTSGAGRGDDPQDPQVLLSSPATQVCFEEPQPSTSTSDLFPTASTSSMEPTSAGRGPPSSSSSRGTDISPKGCGPSVTSDEISSFPLTLPDREGASFSLSEPQDQEDLEPVKKRVKAVGEPDLNLPILVTDPGRDPPAALDVRAEAMKPDKMEETLTCIICQDLLHDCVSLQPCMHTFCAACYSGWMERSALCPTCRCPVERICKNHILNNLVEAYLLQHPDKRRSEEDLRSMAARNKITQDMLQPKVRRAFSDEEGSSEDLLELSDVDSESSDVSQPSIVCRQCPEYRRQAGQPLPYPGPGSEPGAPQVPGDAPSTSASVMAAQDYVCALQGSHAICTCCFQPMPDRRAERERDPRIAPQQCAICLQPFCHLYWGCARTGCLGCLAPFCELNLGDRCLDGVLSNNNYESDVLKNYLATRGLTWKNMLTESLGALQRGVFLLSDYRITGNTVLCYCCGLRSFRELTYQYRQNIPASELPAAVTSRPDCYWGRNCRTQVKAHHAMKFNHICEQTRFKN

Target ORF information:

RefSeq Version	NM_001205474.3
Organism	Bos taurus(cattle)
Definition	Bos taurus checkpoint with forkhead and ring finger domains (CHFR), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001205474.3

ORF Insert Sequence:

ATGGAGCGGC CGGGGGAAGG CGAGCAGCCG CCCCAGCAGC AGCCGTGGGG AAGGCTTCTT 
CGCCTGGGCG CCGAGGAGGG CGAGCCGCAC GTCCTCCTGA GGAAACGGGA GTGGACCATC 
GGGCGAAGAA GAGGTTGTGA CCTTTCATTC CCTGGCAATA AATTGGTCTC TGGAGATCAC 
TGTAAAATTA CAGTGGATGA AAAATCGGGT CAGGTATCAC TGGAAGATAC CAGTGCCAAT 
GGAACTGTCA TTAACAAGCT GAAGGTTGTT AAGAAGCAAA CTTGCCCTTT ACAGACTGGG 
GATGTCATCT ACTTGGTGTA CAGGAAGAAT GAGCCAGAAC ACAACGTGGC ATACCTCTAC 
GAATCTCTAA ATGAAAAGCA AGGTGTAACA CAAGACTCCT TTGAAGCTAA TAAAGAAAAT 
GTGTTCCACG TGACCAAAGA TACCTCAGGT GCAGGGCGAG GTGACGATCC TCAGGATCCC 
CAGGTCCTGC TGTCGTCGCC CGCCACTCAG GTGTGCTTTG AGGAACCACA GCCATCGACG 
TCCACATCGG ACCTCTTCCC CACGGCCTCT ACCTCTTCCA TGGAGCCCAC CTCCGCAGGC 
CGAGGGCCTC CCTCCAGCTC CAGCTCCAGA GGTACAGACA TCTCTCCAAA GGGGTGTGGT 
CCATCTGTGA CAAGTGATGA AATCTCCAGC TTCCCTTTAA CTCTCCCAGA CAGAGAGGGG 
GCTTCCTTTT CTCTGTCGGA ACCCCAGGAT CAGGAGGATT TGGAGCCTGT TAAGAAGAGA 
GTAAAAGCAG TTGGGGAGCC AGACCTTAAC CTGCCCATAC TGGTCACAGA CCCAGGCAGA 
GACCCTCCCG CTGCCCTTGA CGTCAGAGCC GAGGCCATGA AGCCAGACAA GATGGAGGAG 
ACGCTCACGT GCATCATCTG CCAGGACCTG CTGCACGACT GTGTGAGCCT GCAGCCCTGT 
ATGCACACCT TCTGCGCTGC CTGCTACTCG GGCTGGATGG AGCGGTCTGC CCTGTGCCCC 
ACCTGCCGCT GTCCGGTTGA GCGCATCTGT AAAAACCACA TCCTCAACAA CCTGGTGGAG 
GCGTACCTGC TGCAGCACCC GGACAAGAGG CGCAGCGAGG AGGATCTGCG CAGCATGGCC 
GCCAGGAACA AGATCACACA GGACATGCTG CAGCCCAAGG TCAGGAGGGC CTTCTCCGAC 
GAGGAGGGCA GCTCTGAGGA CCTGCTGGAG CTGTCGGACG TGGACAGTGA GTCCTCGGAC 
GTTAGCCAGC CGTCCATCGT GTGCAGACAG TGCCCTGAGT ACCGCAGGCA GGCGGGGCAG 
CCCCTTCCCT ACCCAGGGCC CGGCAGCGAG CCAGGGGCAC CGCAGGTCCC CGGGGATGCA 
CCGTCCACGT CCGCCAGTGT CATGGCAGCC CAGGACTACG TGTGCGCCCT TCAAGGAAGC 
CACGCCATAT GCACCTGCTG CTTCCAGCCC ATGCCGGACC GGAGAGCGGA GCGTGAGCGG 
GACCCCCGCA TCGCCCCCCA GCAGTGTGCC ATCTGCCTGC AGCCCTTCTG CCACCTATAC 
TGGGGCTGTG CCCGGACTGG CTGCCTCGGC TGCCTGGCCC CGTTCTGCGA GCTCAACCTG 
GGTGACCGGT GTCTGGACGG GGTGCTGAGC AACAACAACT ATGAGTCGGA CGTCCTGAAG 
AATTACCTGG CCACCAGGGG TTTGACTTGG AAAAACATGT TGACTGAGAG CCTCGGGGCT 
CTGCAGAGGG GAGTATTCCT GCTGTCTGAT TACAGAATCA CCGGGAACAC CGTGCTGTGC 
TACTGCTGTG GCCTGCGGAG CTTCCGCGAG CTCACCTATC AGTATCGGCA GAACATTCCT 
GCTTCTGAGC TACCAGCGGC CGTCACGTCC CGTCCTGATT GCTACTGGGG CCGCAACTGC 
CGCACTCAGG TGAAGGCCCA CCACGCAATG AAATTCAATC ACATCTGTGA ACAGACGAGG 
TTCAAGAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHFR ( XM_005217679.4 ) cDNA ORF clone, Bos taurus(Cattle) -> XP_005217736.2 Bos taurus checkpoint with forkhead and ring finger domains (CHFR), transcript variant X1, mRNA.

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CloneID	OBa224391
Clone ID Related Accession (Same CDS sequence)	XM_005217679.4 , NM_001205474.3
Accession Version	XM_005217679.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1992bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase CHFR isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005217679.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCGGC CGGGGGAAGG CGAGCAGCCG CCCCAGCAGC AGCCGTGGGG AAGGCTTCTT 
CGCCTGGGCG CCGAGGAGGG CGAGCCGCAC GTCCTCCTGA GGAAACGGGA GTGGACCATC 
GGGCGAAGAA GAGGTTGTGA CCTTTCATTC CCTGGCAATA AATTGGTCTC TGGAGATCAC 
TGTAAAATTA CAGTGGATGA AAAATCGGGT CAGGTATCAC TGGAAGATAC CAGTGCCAAT 
GGAACTGTCA TTAACAAGCT GAAGGTTGTT AAGAAGCAAA CTTGCCCTTT ACAGACTGGG 
GATGTCATCT ACTTGGTGTA CAGGAAGAAT GAGCCAGAAC ACAACGTGGC ATACCTCTAC 
GAATCTCTAA ATGAAAAGCA AGGTGTAACA CAAGACTCCT TTGAAGCTAA TAAAGAAAAT 
GTGTTCCACG TGACCAAAGA TACCTCAGGT GCAGGGCGAG GTGACGATCC TCAGGATCCC 
CAGGTCCTGC TGTCGTCGCC CGCCACTCAG GTGTGCTTTG AGGAACCACA GCCATCGACG 
TCCACATCGG ACCTCTTCCC CACGGCCTCT ACCTCTTCCA TGGAGCCCAC CTCCGCAGGC 
CGAGGGCCTC CCTCCAGCTC CAGCTCCAGA GGTACAGACA TCTCTCCAAA GGGGTGTGGT 
CCATCTGTGA CAAGTGATGA AATCTCCAGC TTCCCTTTAA CTCTCCCAGA CAGAGAGGGG 
GCTTCCTTTT CTCTGTCGGA ACCCCAGGAT CAGGAGGATT TGGAGCCTGT TAAGAAGAGA 
GTAAAAGCAG TTGGGGAGCC AGACCTTAAC CTGCCCATAC TGGTCACAGA CCCAGGCAGA 
GACCCTCCCG CTGCCCTTGA CGTCAGAGCC GAGGCCATGA AGCCAGACAA GATGGAGGAG 
ACGCTCACGT GCATCATCTG CCAGGACCTG CTGCACGACT GTGTGAGCCT GCAGCCCTGT 
ATGCACACCT TCTGCGCTGC CTGCTACTCG GGCTGGATGG AGCGGTCTGC CCTGTGCCCC 
ACCTGCCGCT GTCCGGTTGA GCGCATCTGT AAAAACCACA TCCTCAACAA CCTGGTGGAG 
GCGTACCTGC TGCAGCACCC GGACAAGAGG CGCAGCGAGG AGGATCTGCG CAGCATGGCC 
GCCAGGAACA AGATCACACA GGACATGCTG CAGCCCAAGG TCAGGAGGGC CTTCTCCGAC 
GAGGAGGGCA GCTCTGAGGA CCTGCTGGAG CTGTCGGACG TGGACAGTGA GTCCTCGGAC 
GTTAGCCAGC CGTCCATCGT GTGCAGACAG TGCCCTGAGT ACCGCAGGCA GGCGGGGCAG 
CCCCTTCCCT ACCCAGGGCC CGGCAGCGAG CCAGGGGCAC CGCAGGTCCC CGGGGATGCA 
CCGTCCACGT CCGCCAGTGT CATGGCAGCC CAGGACTACG TGTGCGCCCT TCAAGGAAGC 
CACGCCATAT GCACCTGCTG CTTCCAGCCC ATGCCGGACC GGAGAGCGGA GCGTGAGCGG 
GACCCCCGCA TCGCCCCCCA GCAGTGTGCC ATCTGCCTGC AGCCCTTCTG CCACCTATAC 
TGGGGCTGTG CCCGGACTGG CTGCCTCGGC TGCCTGGCCC CGTTCTGCGA GCTCAACCTG 
GGTGACCGGT GTCTGGACGG GGTGCTGAGC AACAACAACT ATGAGTCGGA CGTCCTGAAG 
AATTACCTGG CCACCAGGGG TTTGACTTGG AAAAACATGT TGACTGAGAG CCTCGGGGCT 
CTGCAGAGGG GAGTATTCCT GCTGTCTGAT TACAGAATCA CCGGGAACAC CGTGCTGTGC 
TACTGCTGTG GCCTGCGGAG CTTCCGCGAG CTCACCTATC AGTATCGGCA GAACATTCCT 
GCTTCTGAGC TACCAGCGGC CGTCACGTCC CGTCCTGATT GCTACTGGGG CCGCAACTGC 
CGCACTCAGG TGAAGGCCCA CCACGCAATG AAATTCAATC ACATCTGTGA ACAGACGAGG 
TTCAAGAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005217736.2
CDS	154..2145
Translation	MERPGEGEQPPQQQPWGRLLRLGAEEGEPHVLLRKREWTIGRRRGCDLSFPGNKLVSGDHCKITVDEKSGQVSLEDTSANGTVINKLKVVKKQTCPLQTGDVIYLVYRKNEPEHNVAYLYESLNEKQGVTQDSFEANKENVFHVTKDTSGAGRGDDPQDPQVLLSSPATQVCFEEPQPSTSTSDLFPTASTSSMEPTSAGRGPPSSSSSRGTDISPKGCGPSVTSDEISSFPLTLPDREGASFSLSEPQDQEDLEPVKKRVKAVGEPDLNLPILVTDPGRDPPAALDVRAEAMKPDKMEETLTCIICQDLLHDCVSLQPCMHTFCAACYSGWMERSALCPTCRCPVERICKNHILNNLVEAYLLQHPDKRRSEEDLRSMAARNKITQDMLQPKVRRAFSDEEGSSEDLLELSDVDSESSDVSQPSIVCRQCPEYRRQAGQPLPYPGPGSEPGAPQVPGDAPSTSASVMAAQDYVCALQGSHAICTCCFQPMPDRRAERERDPRIAPQQCAICLQPFCHLYWGCARTGCLGCLAPFCELNLGDRCLDGVLSNNNYESDVLKNYLATRGLTWKNMLTESLGALQRGVFLLSDYRITGNTVLCYCCGLRSFRELTYQYRQNIPASELPAAVTSRPDCYWGRNCRTQVKAHHAMKFNHICEQTRFKN

Target ORF information:

RefSeq Version	XM_005217679.4
Organism	Bos taurus(cattle)
Definition	Bos taurus checkpoint with forkhead and ring finger domains (CHFR), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005217679.4

ORF Insert Sequence:

ATGGAGCGGC CGGGGGAAGG CGAGCAGCCG CCCCAGCAGC AGCCGTGGGG AAGGCTTCTT 
CGCCTGGGCG CCGAGGAGGG CGAGCCGCAC GTCCTCCTGA GGAAACGGGA GTGGACCATC 
GGGCGAAGAA GAGGTTGTGA CCTTTCATTC CCTGGCAATA AATTGGTCTC TGGAGATCAC 
TGTAAAATTA CAGTGGATGA AAAATCGGGT CAGGTATCAC TGGAAGATAC CAGTGCCAAT 
GGAACTGTCA TTAACAAGCT GAAGGTTGTT AAGAAGCAAA CTTGCCCTTT ACAGACTGGG 
GATGTCATCT ACTTGGTGTA CAGGAAGAAT GAGCCAGAAC ACAACGTGGC ATACCTCTAC 
GAATCTCTAA ATGAAAAGCA AGGTGTAACA CAAGACTCCT TTGAAGCTAA TAAAGAAAAT 
GTGTTCCACG TGACCAAAGA TACCTCAGGT GCAGGGCGAG GTGACGATCC TCAGGATCCC 
CAGGTCCTGC TGTCGTCGCC CGCCACTCAG GTGTGCTTTG AGGAACCACA GCCATCGACG 
TCCACATCGG ACCTCTTCCC CACGGCCTCT ACCTCTTCCA TGGAGCCCAC CTCCGCAGGC 
CGAGGGCCTC CCTCCAGCTC CAGCTCCAGA GGTACAGACA TCTCTCCAAA GGGGTGTGGT 
CCATCTGTGA CAAGTGATGA AATCTCCAGC TTCCCTTTAA CTCTCCCAGA CAGAGAGGGG 
GCTTCCTTTT CTCTGTCGGA ACCCCAGGAT CAGGAGGATT TGGAGCCTGT TAAGAAGAGA 
GTAAAAGCAG TTGGGGAGCC AGACCTTAAC CTGCCCATAC TGGTCACAGA CCCAGGCAGA 
GACCCTCCCG CTGCCCTTGA CGTCAGAGCC GAGGCCATGA AGCCAGACAA GATGGAGGAG 
ACGCTCACGT GCATCATCTG CCAGGACCTG CTGCACGACT GTGTGAGCCT GCAGCCCTGT 
ATGCACACCT TCTGCGCTGC CTGCTACTCG GGCTGGATGG AGCGGTCTGC CCTGTGCCCC 
ACCTGCCGCT GTCCGGTTGA GCGCATCTGT AAAAACCACA TCCTCAACAA CCTGGTGGAG 
GCGTACCTGC TGCAGCACCC GGACAAGAGG CGCAGCGAGG AGGATCTGCG CAGCATGGCC 
GCCAGGAACA AGATCACACA GGACATGCTG CAGCCCAAGG TCAGGAGGGC CTTCTCCGAC 
GAGGAGGGCA GCTCTGAGGA CCTGCTGGAG CTGTCGGACG TGGACAGTGA GTCCTCGGAC 
GTTAGCCAGC CGTCCATCGT GTGCAGACAG TGCCCTGAGT ACCGCAGGCA GGCGGGGCAG 
CCCCTTCCCT ACCCAGGGCC CGGCAGCGAG CCAGGGGCAC CGCAGGTCCC CGGGGATGCA 
CCGTCCACGT CCGCCAGTGT CATGGCAGCC CAGGACTACG TGTGCGCCCT TCAAGGAAGC 
CACGCCATAT GCACCTGCTG CTTCCAGCCC ATGCCGGACC GGAGAGCGGA GCGTGAGCGG 
GACCCCCGCA TCGCCCCCCA GCAGTGTGCC ATCTGCCTGC AGCCCTTCTG CCACCTATAC 
TGGGGCTGTG CCCGGACTGG CTGCCTCGGC TGCCTGGCCC CGTTCTGCGA GCTCAACCTG 
GGTGACCGGT GTCTGGACGG GGTGCTGAGC AACAACAACT ATGAGTCGGA CGTCCTGAAG 
AATTACCTGG CCACCAGGGG TTTGACTTGG AAAAACATGT TGACTGAGAG CCTCGGGGCT 
CTGCAGAGGG GAGTATTCCT GCTGTCTGAT TACAGAATCA CCGGGAACAC CGTGCTGTGC 
TACTGCTGTG GCCTGCGGAG CTTCCGCGAG CTCACCTATC AGTATCGGCA GAACATTCCT 
GCTTCTGAGC TACCAGCGGC CGTCACGTCC CGTCCTGATT GCTACTGGGG CCGCAACTGC 
CGCACTCAGG TGAAGGCCCA CCACGCAATG AAATTCAATC ACATCTGTGA ACAGACGAGG 
TTCAAGAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHFR ( XM_024977446.1 ) cDNA ORF clone, Bos taurus(Cattle) -> XP_024833214.1 Bos taurus checkpoint with forkhead and ring finger domains (CHFR), transcript variant X2, mRNA.

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CloneID	OBa224392
Clone ID Related Accession (Same CDS sequence)	XM_024977446.1
Accession Version	XM_024977446.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1884bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase CHFR isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCGGC CGGGGGAAGG CGAGCAGCCG CCCCAGCAGC AGCCGTGGGG AAGGCTTCTT 
CGCCTGGGCG CCGAGGAGGG CGAGCCGCAC GTCCTCCTGA GGAAACGGGA GTGGACCATC 
GGGCGAAGAA GAGGTTGTGA CCTTTCATTC CCTGGCAATA AATTGGTCTC TGGAGATCAC 
TGTAAAATTA CAGTGGATGA AAAATCGGGT CAGGTATCAC TGGAAGATAC CAGTGCCAAT 
GGAACTGTCA TTAACAAGCT GAAGGTTGTT AAGAAGCAAA CTTGCCCTTT ACAGACTGGG 
GATGTCATCT ACTTGGTGTA CAGGAAGAAT GAGCCAGAAC ACAACGTGGC ATACCTCTAC 
GAATCTCTAA ATGAAAAGCA AGGTGTAACA CAAGACTCCT TTGAAGCTAA TAAAGAAAAT 
GTGTTCCACG TGACCAAAGA TACCTCAGGT GCAGGGCGAG GTGACGATCC TCAGGATCCC 
CAGGTCCTGC TGTCGTCGCC CGCCACTCAG GTGTGCTTTG AGGAACCACA GCCATCGACG 
TCCACATCGG ACCTCTTCCC CACGGCCTCT ACCTCTTCCA TGGAGCCCAC CTCCGCAGGC 
CGAGGGCCTC CCTCCAGCTC CAGCTCCAGA GGTACAGACA TCTCTCCAAA GGGGTGTGGT 
CCATCTGTGA CAAGTGATGA AATCTCCAGC TTCCCTTTAA CTCTCCCAGA CAGAGAGGGG 
GCTTCCTTTT CTCTGTCGGA ACCCCAGGAT CAGGAGGATT TGGAGCCTGT TAAGAAGAGA 
GTAAAAGCAG TTGGGGAGCC AGACCTTAAC CTGCCCATAC TGGTCACAGA CCCAGGCAGA 
GACCCTCCCG CTGCCCTTGA CGTCAGAGCC GAGGCCATGA AGCCAGACAA GATGGAGGAG 
ACGCTCACGT GCATCATCTG CCAGGACCTG CTGCACGACT GTGTGAGCCT GCAGCCCTGT 
ATGCACACCT TCTGCGCTGC CTGCTACTCG GGCTGGATGG AGCGGTCTGC CCTGTGCCCC 
ACCTGCCGCT GTCCGGTTGA GCGCATCTGT AAAAACCACA TCCTCAACAA CCTGGTGGAG 
GCGTACCTGC TGCAGCACCC GGACAAGAGG CGCAGCGAGG AGGATCTGCG CAGCATGGCC 
GCCAGGAACA AGATCACACA GGACATGCTG CAGCCCAAGG TCAGGAGGGC CTTCTCCGAC 
GAGGAGGGCA GCTCTGAGGA CCTGCTGGAG CTGTCGGACG TGGACAGTGA GTCCTCGGAC 
GTTAGCCAGC CGTCCATCGT GTGCAGACAG TGCCCTGAGT ACCGCAGGCA GGCGGGGCAG 
CCCCTTCCCT ACCCAGGGCC CGGCAGCGAG CCAGGGGCAC CGCAGGTCCC CGGGGATGCA 
CCGTCCACGT CCGCCAGTGT CATGGCAGCC CAGGACTACG TGTGCGCCCT TCAAGGAAGC 
CACGCCATAT GCACCTGCTG CTTCCAGCCC ATGCCGGACC GGAGAGCGGA GCGTGAGCGG 
GACCCCCGCA TCGCCCCCCA GCAGTGTGCC ATCTGCCTGC AGCCCTTCTG CCACCTATAC 
TGGGGCTGTG CCCGGACTGG CTGCCTCGGC TGCCTGGCCC CGTTCTGCGA GCTCAACCTG 
GGTGACCGGT GTCTGGACGG GGTGCTGAGC AACAACAACT ATGAGTCGGA CGTCCTGAAG 
AATTACCTGG CCACCAGGGG TTTGACTTGG AAAAACATGT TGACTGAGAG CCTCGGGGCT 
CTGCAGAGGG GAGTATTCCT GCTGTCTGCG GCCGTCACGT CCCGTCCTGA TTGCTACTGG 
GGCCGCAACT GCCGCACTCA GGTGAAGGCC CACCACGCAA TGAAATTCAA TCACATCTGT 
GAACAGACGA GGTTCAAGAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024833214.1
CDS	144..2027
Translation	MERPGEGEQPPQQQPWGRLLRLGAEEGEPHVLLRKREWTIGRRRGCDLSFPGNKLVSGDHCKITVDEKSGQVSLEDTSANGTVINKLKVVKKQTCPLQTGDVIYLVYRKNEPEHNVAYLYESLNEKQGVTQDSFEANKENVFHVTKDTSGAGRGDDPQDPQVLLSSPATQVCFEEPQPSTSTSDLFPTASTSSMEPTSAGRGPPSSSSSRGTDISPKGCGPSVTSDEISSFPLTLPDREGASFSLSEPQDQEDLEPVKKRVKAVGEPDLNLPILVTDPGRDPPAALDVRAEAMKPDKMEETLTCIICQDLLHDCVSLQPCMHTFCAACYSGWMERSALCPTCRCPVERICKNHILNNLVEAYLLQHPDKRRSEEDLRSMAARNKITQDMLQPKVRRAFSDEEGSSEDLLELSDVDSESSDVSQPSIVCRQCPEYRRQAGQPLPYPGPGSEPGAPQVPGDAPSTSASVMAAQDYVCALQGSHAICTCCFQPMPDRRAERERDPRIAPQQCAICLQPFCHLYWGCARTGCLGCLAPFCELNLGDRCLDGVLSNNNYESDVLKNYLATRGLTWKNMLTESLGALQRGVFLLSAAVTSRPDCYWGRNCRTQVKAHHAMKFNHICEQTRFKN

Target ORF information:

RefSeq Version	XM_024977446.1
Organism	Bos taurus(cattle)
Definition	Bos taurus checkpoint with forkhead and ring finger domains (CHFR), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024977446.1

ORF Insert Sequence:

ATGGAGCGGC CGGGGGAAGG CGAGCAGCCG CCCCAGCAGC AGCCGTGGGG AAGGCTTCTT 
CGCCTGGGCG CCGAGGAGGG CGAGCCGCAC GTCCTCCTGA GGAAACGGGA GTGGACCATC 
GGGCGAAGAA GAGGTTGTGA CCTTTCATTC CCTGGCAATA AATTGGTCTC TGGAGATCAC 
TGTAAAATTA CAGTGGATGA AAAATCGGGT CAGGTATCAC TGGAAGATAC CAGTGCCAAT 
GGAACTGTCA TTAACAAGCT GAAGGTTGTT AAGAAGCAAA CTTGCCCTTT ACAGACTGGG 
GATGTCATCT ACTTGGTGTA CAGGAAGAAT GAGCCAGAAC ACAACGTGGC ATACCTCTAC 
GAATCTCTAA ATGAAAAGCA AGGTGTAACA CAAGACTCCT TTGAAGCTAA TAAAGAAAAT 
GTGTTCCACG TGACCAAAGA TACCTCAGGT GCAGGGCGAG GTGACGATCC TCAGGATCCC 
CAGGTCCTGC TGTCGTCGCC CGCCACTCAG GTGTGCTTTG AGGAACCACA GCCATCGACG 
TCCACATCGG ACCTCTTCCC CACGGCCTCT ACCTCTTCCA TGGAGCCCAC CTCCGCAGGC 
CGAGGGCCTC CCTCCAGCTC CAGCTCCAGA GGTACAGACA TCTCTCCAAA GGGGTGTGGT 
CCATCTGTGA CAAGTGATGA AATCTCCAGC TTCCCTTTAA CTCTCCCAGA CAGAGAGGGG 
GCTTCCTTTT CTCTGTCGGA ACCCCAGGAT CAGGAGGATT TGGAGCCTGT TAAGAAGAGA 
GTAAAAGCAG TTGGGGAGCC AGACCTTAAC CTGCCCATAC TGGTCACAGA CCCAGGCAGA 
GACCCTCCCG CTGCCCTTGA CGTCAGAGCC GAGGCCATGA AGCCAGACAA GATGGAGGAG 
ACGCTCACGT GCATCATCTG CCAGGACCTG CTGCACGACT GTGTGAGCCT GCAGCCCTGT 
ATGCACACCT TCTGCGCTGC CTGCTACTCG GGCTGGATGG AGCGGTCTGC CCTGTGCCCC 
ACCTGCCGCT GTCCGGTTGA GCGCATCTGT AAAAACCACA TCCTCAACAA CCTGGTGGAG 
GCGTACCTGC TGCAGCACCC GGACAAGAGG CGCAGCGAGG AGGATCTGCG CAGCATGGCC 
GCCAGGAACA AGATCACACA GGACATGCTG CAGCCCAAGG TCAGGAGGGC CTTCTCCGAC 
GAGGAGGGCA GCTCTGAGGA CCTGCTGGAG CTGTCGGACG TGGACAGTGA GTCCTCGGAC 
GTTAGCCAGC CGTCCATCGT GTGCAGACAG TGCCCTGAGT ACCGCAGGCA GGCGGGGCAG 
CCCCTTCCCT ACCCAGGGCC CGGCAGCGAG CCAGGGGCAC CGCAGGTCCC CGGGGATGCA 
CCGTCCACGT CCGCCAGTGT CATGGCAGCC CAGGACTACG TGTGCGCCCT TCAAGGAAGC 
CACGCCATAT GCACCTGCTG CTTCCAGCCC ATGCCGGACC GGAGAGCGGA GCGTGAGCGG 
GACCCCCGCA TCGCCCCCCA GCAGTGTGCC ATCTGCCTGC AGCCCTTCTG CCACCTATAC 
TGGGGCTGTG CCCGGACTGG CTGCCTCGGC TGCCTGGCCC CGTTCTGCGA GCTCAACCTG 
GGTGACCGGT GTCTGGACGG GGTGCTGAGC AACAACAACT ATGAGTCGGA CGTCCTGAAG 
AATTACCTGG CCACCAGGGG TTTGACTTGG AAAAACATGT TGACTGAGAG CCTCGGGGCT 
CTGCAGAGGG GAGTATTCCT GCTGTCTGCG GCCGTCACGT CCCGTCCTGA TTGCTACTGG 
GGCCGCAACT GCCGCACTCA GGTGAAGGCC CACCACGCAA TGAAATTCAA TCACATCTGT 
GAACAGACGA GGTTCAAGAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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CHFR cDNA ORF clone, Bos taurus(Cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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