Chfr gene cDNA ORF clone, Mus musculus(house mouse)

mRNA	Protein	Name
XM_011249446.2	XP_011247748.1	E3 ubiquitin-protein ligase CHFR isoform X2
NM_172717.4	NP_766305.2	E3 ubiquitin-protein ligase CHFR isoform 2
XM_011249445.2	XP_011247747.1	E3 ubiquitin-protein ligase CHFR isoform X2
XM_030254421.1	XP_030110281.1	E3 ubiquitin-protein ligase CHFR isoform X5
NM_001289580.1	NP_001276509.1	E3 ubiquitin-protein ligase CHFR isoform 5
NM_001289579.1	NP_001276508.1	E3 ubiquitin-protein ligase CHFR isoform 4
NM_001289578.1	NP_001276507.1	E3 ubiquitin-protein ligase CHFR isoform 3
XM_030254419.1	XP_030110279.1	E3 ubiquitin-protein ligase CHFR isoform X3
XM_030254418.1	XP_030110278.1	E3 ubiquitin-protein ligase CHFR isoform X3
NM_001289577.1	NP_001276506.1	E3 ubiquitin-protein ligase CHFR isoform 1
XM_006534915.1	XP_006534978.1	E3 ubiquitin-protein ligase CHFR isoform X1
XM_030254420.1	XP_030110280.1	E3 ubiquitin-protein ligase CHFR isoform X4

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu16350	NM_001289579.1 Latest version!	Mus musculus checkpoint with forkhead and ring finger domains (Chfr), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu16449	NM_001289578.1 Latest version!	Mus musculus checkpoint with forkhead and ring finger domains (Chfr), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu16462	NM_001289580.1 Latest version!	Mus musculus checkpoint with forkhead and ring finger domains (Chfr), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu16505	NM_172717.4 Latest version!	Mus musculus checkpoint with forkhead and ring finger domains (Chfr), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$391.30 $559.00
OMu17144	NM_001289577.1 Latest version!	Mus musculus checkpoint with forkhead and ring finger domains (Chfr), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu16350	XM_011249446.2 Latest version!	Mus musculus checkpoint with forkhead and ring finger domains (Chfr), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu16350	XM_011249445.2 Latest version!	Mus musculus checkpoint with forkhead and ring finger domains (Chfr), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu35453	XM_006534915.1 Latest version!	Mus musculus checkpoint with forkhead and ring finger domains (Chfr), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu88879	XM_030254420.1 Latest version!	Mus musculus checkpoint with forkhead and ring finger domains (Chfr), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu88880	XM_030254421.1 Latest version!	Mus musculus checkpoint with forkhead and ring finger domains (Chfr), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu16462	XM_030254418.1 Latest version!	Mus musculus checkpoint with forkhead and ring finger domains (Chfr), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu16462	XM_030254419.1 Latest version!	Mus musculus checkpoint with forkhead and ring finger domains (Chfr), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

Chfr ( NM_001289579.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001276508.1 Mus musculus checkpoint with forkhead and ring finger domains (Chfr), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OMu16350
Clone ID Related Accession (Same CDS sequence)	XM_011249446.2 , XM_011249445.2 , NM_001289579.1
Accession Version	NM_001289579.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1575bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase CHFR isoform 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC118260.7 and AK169182.1. On Jan 24, 2014 this sequence version replaced XM_006534918.1. Transcript Variant: This variant (4) contains an alternate exon in the 5' region and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (4) has a shorter N-terminus than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK169182.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849381 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTCCATG TGACCAAAGA TTGCTCAGGT CCAGGGCAGG GTGATGATCC CCAGGTTCCA 
CTATTGTCAC CCATGGCTCA GACATGCTTA GAGGAACCAC AGCCATCAAC ATCGACATCA 
GACCTCCTCC CCACGGCCTC TACCTCTTCT ACGGAGCCAG AGCTGACCTC TGCAGGGCAA 
AAGCATTCTT CTAGCTCTGG ACCTGGGAAC ACAAGCATCT CCCCAAAAGG ACGCAGTTCA 
CTTGTTGCAA ATGGCGAACT CTCTAGCCTT TCTCCAGTTT TCCAAGACAA AGAAGCATCC 
TTTTCTTTGC TGGAAAGTAA AGACCATGAG GAATTGGAGC CTGCCAAAAA AAAGATGAAA 
GGAGATGGGG AACTTGACAC GAACCTCCAG TTATTAGTTT CAGGCCAGCG TGGAAATGCC 
CAAACCTCAA GTGAAGATGT CAAAGATGCC TCTGTGAAGC CAGACAAGAT GGAGGAGACA 
CTAACCTGTA TCATCTGCCA GGACCTTCTG CACGATTGTG TGAGTTTGCA GCCTTGTATG 
CACACATTTT GTGCGGCTTG CTACTCTGGT TGGATGGAGC GTTCATCTCT GTGCCCTACC 
TGCCGATGTC CAGTGGAGCG GATTTGCAAA AACCACATCC TGAACAACCT AGTGGAAGCA 
TACCTTATCC AGCACCCAGA TAAAAGTCGC AGTGAAGAAG ATGTGAGAAG TATGGATGCA 
AGGAATAAAA TCACTCAAGA TATGCTGCAA CCCAAAGTCA GGAGGTCTTT CTCTGATGAA 
GAGGGGAGTT CAGAGGACCT GCTAGAGCTG TCTGATGTCG ACAGTGAATC CTCAGATATC 
AGTCAGCCAT ACATTGTCTG CAGACAGTGT CCTGAATACA GAAGGCAAGC GGTGCAGTCT 
CTTCCTTGCC CAGTCCCAGA GAGTGAGCTG GGAGCTACAC TGGCCCTTGG TGGGGAGGCA 
CCTTCAACAT CTGCCAGCTT GCCAACAGCA GCCCCGGATT ACATGTGCCC TCTTCAAGGA 
AGCCATGCCA TATGCACCTG CTGCTTCCAG CCTATGCCTG ACCGGAGAGC TGAACGGGAG 
CAGGATCCCC GCGTCGCCCC TCAGCAGTGT GCGGTGTGCC TGCAGCCCTT CTGCCACCTG 
TACTGGGGCT GCACGAGGAC TGGCTGCTTT GGCTGCTTGG CCCCATTCTG TGAGCTCAAC 
CTGGGGGACA AGTGCTTGGA TGGAGTGCTG AACAATAACA ATTATGAATC GGACATCCTG 
AAGAATTACC TGGCAACCAG GGGTCTGACA TGGAAAAGTG TGTTGACAGA GAGTCTCCTG 
GCTCTGCAGC GAGGTGTATT TATGCTGTCT GATTACAGAA TCACTGGAAA TACTGTGCTG 
TGTTACTGCT GTGGTCTGCG TAGCTTCCGA GAGCTGACCT ACCAGTATCG TCAGAACATT 
CCTGCTTCTG AGTTGCCAGT GACTGTAACA TCCCGTCCTG ACTGCTACTG GGGCCGTAAC 
TGTCGCACTC AGGTGAAGGC TCACCATGCA ATGAAATTCA ATCACATCTG TGAGCAAACA 
AGGTTCAAGA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001276508.1
CDS	610..2184
Translation	MFHVTKDCSGPGQGDDPQVPLLSPMAQTCLEEPQPSTSTSDLLPTASTSSTEPELTSAGQKHSSSSGPGNTSISPKGRSSLVANGELSSLSPVFQDKEASFSLLESKDHEELEPAKKKMKGDGELDTNLQLLVSGQRGNAQTSSEDVKDASVKPDKMEETLTCIICQDLLHDCVSLQPCMHTFCAACYSGWMERSSLCPTCRCPVERICKNHILNNLVEAYLIQHPDKSRSEEDVRSMDARNKITQDMLQPKVRRSFSDEEGSSEDLLELSDVDSESSDISQPYIVCRQCPEYRRQAVQSLPCPVPESELGATLALGGEAPSTSASLPTAAPDYMCPLQGSHAICTCCFQPMPDRRAEREQDPRVAPQQCAVCLQPFCHLYWGCTRTGCFGCLAPFCELNLGDKCLDGVLNNNNYESDILKNYLATRGLTWKSVLTESLLALQRGVFMLSDYRITGNTVLCYCCGLRSFRELTYQYRQNIPASELPVTVTSRPDCYWGRNCRTQVKAHHAMKFNHICEQTRFKN

Target ORF information:

RefSeq Version	NM_001289579.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus checkpoint with forkhead and ring finger domains (Chfr), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001289579.1

ORF Insert Sequence:

ATGTTCCATG TGACCAAAGA TTGCTCAGGT CCAGGGCAGG GTGATGATCC CCAGGTTCCA 
CTATTGTCAC CCATGGCTCA GACATGCTTA GAGGAACCAC AGCCATCAAC ATCGACATCA 
GACCTCCTCC CCACGGCCTC TACCTCTTCT ACGGAGCCAG AGCTGACCTC TGCAGGGCAA 
AAGCATTCTT CTAGCTCTGG ACCTGGGAAC ACAAGCATCT CCCCAAAAGG ACGCAGTTCA 
CTTGTTGCAA ATGGCGAACT CTCTAGCCTT TCTCCAGTTT TCCAAGACAA AGAAGCATCC 
TTTTCTTTGC TGGAAAGTAA AGACCATGAG GAATTGGAGC CTGCCAAAAA AAAGATGAAA 
GGAGATGGGG AACTTGACAC GAACCTCCAG TTATTAGTTT CAGGCCAGCG TGGAAATGCC 
CAAACCTCAA GTGAAGATGT CAAAGATGCC TCTGTGAAGC CAGACAAGAT GGAGGAGACA 
CTAACCTGTA TCATCTGCCA GGACCTTCTG CACGATTGTG TGAGTTTGCA GCCTTGTATG 
CACACATTTT GTGCGGCTTG CTACTCTGGT TGGATGGAGC GTTCATCTCT GTGCCCTACC 
TGCCGATGTC CAGTGGAGCG GATTTGCAAA AACCACATCC TGAACAACCT AGTGGAAGCA 
TACCTTATCC AGCACCCAGA TAAAAGTCGC AGTGAAGAAG ATGTGAGAAG TATGGATGCA 
AGGAATAAAA TCACTCAAGA TATGCTGCAA CCCAAAGTCA GGAGGTCTTT CTCTGATGAA 
GAGGGGAGTT CAGAGGACCT GCTAGAGCTG TCTGATGTCG ACAGTGAATC CTCAGATATC 
AGTCAGCCAT ACATTGTCTG CAGACAGTGT CCTGAATACA GAAGGCAAGC GGTGCAGTCT 
CTTCCTTGCC CAGTCCCAGA GAGTGAGCTG GGAGCTACAC TGGCCCTTGG TGGGGAGGCA 
CCTTCAACAT CTGCCAGCTT GCCAACAGCA GCCCCGGATT ACATGTGCCC TCTTCAAGGA 
AGCCATGCCA TATGCACCTG CTGCTTCCAG CCTATGCCTG ACCGGAGAGC TGAACGGGAG 
CAGGATCCCC GCGTCGCCCC TCAGCAGTGT GCGGTGTGCC TGCAGCCCTT CTGCCACCTG 
TACTGGGGCT GCACGAGGAC TGGCTGCTTT GGCTGCTTGG CCCCATTCTG TGAGCTCAAC 
CTGGGGGACA AGTGCTTGGA TGGAGTGCTG AACAATAACA ATTATGAATC GGACATCCTG 
AAGAATTACC TGGCAACCAG GGGTCTGACA TGGAAAAGTG TGTTGACAGA GAGTCTCCTG 
GCTCTGCAGC GAGGTGTATT TATGCTGTCT GATTACAGAA TCACTGGAAA TACTGTGCTG 
TGTTACTGCT GTGGTCTGCG TAGCTTCCGA GAGCTGACCT ACCAGTATCG TCAGAACATT 
CCTGCTTCTG AGTTGCCAGT GACTGTAACA TCCCGTCCTG ACTGCTACTG GGGCCGTAAC 
TGTCGCACTC AGGTGAAGGC TCACCATGCA ATGAAATTCA ATCACATCTG TGAGCAAACA 
AGGTTCAAGA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chfr ( NM_001289578.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001276507.1 Mus musculus checkpoint with forkhead and ring finger domains (Chfr), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu16449
Clone ID Related Accession (Same CDS sequence)	NM_001289578.1
Accession Version	NM_001289578.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1779bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase CHFR isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC118260.7 and AK147892.1. On Jan 24, 2014 this sequence version replaced XM_006534917.1. Transcript Variant: This variant (3) lacks an alternate in-frame exon, compared to variant 1. The encoded isoform (3) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK147892.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849381 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCTAC ACGGGGAAGA GCAGCCGCCG CCGCCGCAGG AACCCTGGGG GAGGCTTCTT 
CGTCTAGGCG CAGAGGAGGA CGAACCCCAG ATCCTTCTTT GGAAACGCGA GTGGACCATC 
GGAAGGAGGA GAGGCTGTGA CCTCTCTTTC CCCAGCAATA AACTGGTCTC TGGAGATCAC 
TGTAAACTTA CAGTGGATGA AATATCTGGT GAGGTGACAC TGGAAGACAC CAGCACCAAT 
GGAACAGTGA TCAATAAGCT GCAAGTTGTT AAGAAGCAGA CTTACCCTTT ACAGAGCGGG 
GATATCATCT ATTTGGTGTA CAGGAAGAAT GAGCCAGAAC ACAATGTGGC ATACCTCTAT 
GAATCTTTAA GTGGCAAACA GAGCTTAACT CAAGACTCCC TTGGACCTGG GAACACAAGC 
ATCTCCCCAA AAGGACGCAG TTCACTTGTT GCAAATGGCG AACTCTCTAG CCTTTCTCCA 
GTTTTCCAAG ACAAAGAAGC ATCCTTTTCT TTGCTGGAAA GTAAAGACCA TGAGGAATTG 
GAGCCTGCCA AAAAAAAGAT GAAAGGAGAT GGGGAACTTG ACACGAACCT CCAGTTATTA 
GTTTCAGGCC AGCGTGGAAA TGCCCAAACC TCAAGTGAAG ATGTCAAAGA TGCCTCTGTG 
AAGCCAGACA AGATGGAGGA GACACTAACC TGTATCATCT GCCAGGACCT TCTGCACGAT 
TGTGTGAGTT TGCAGCCTTG TATGCACACA TTTTGTGCGG CTTGCTACTC TGGTTGGATG 
GAGCGTTCAT CTCTGTGCCC TACCTGCCGA TGTCCAGTGG AGCGGATTTG CAAAAACCAC 
ATCCTGAACA ACCTAGTGGA AGCATACCTT ATCCAGCACC CAGATAAAAG TCGCAGTGAA 
GAAGATGTGA GAAGTATGGA TGCAAGGAAT AAAATCACTC AAGATATGCT GCAACCCAAA 
GTCAGGAGGT CTTTCTCTGA TGAAGAGGGG AGTTCAGAGG ACCTGCTAGA GCTGTCTGAT 
GTCGACAGTG AATCCTCAGA TATCAGTCAG CCATACATTG TCTGCAGACA GTGTCCTGAA 
TACAGAAGGC AAGCGGTGCA GTCTCTTCCT TGCCCAGTCC CAGAGAGTGA GCTGGGAGCT 
ACACTGGCCC TTGGTGGGGA GGCACCTTCA ACATCTGCCA GCTTGCCAAC AGCAGCCCCG 
GATTACATGT GCCCTCTTCA AGGAAGCCAT GCCATATGCA CCTGCTGCTT CCAGCCTATG 
CCTGACCGGA GAGCTGAACG GGAGCAGGAT CCCCGCGTCG CCCCTCAGCA GTGTGCGGTG 
TGCCTGCAGC CCTTCTGCCA CCTGTACTGG GGCTGCACGA GGACTGGCTG CTTTGGCTGC 
TTGGCCCCAT TCTGTGAGCT CAACCTGGGG GACAAGTGCT TGGATGGAGT GCTGAACAAT 
AACAATTATG AATCGGACAT CCTGAAGAAT TACCTGGCAA CCAGGGGTCT GACATGGAAA 
AGTGTGTTGA CAGAGAGTCT CCTGGCTCTG CAGCGAGGTG TATTTATGCT GTCTGATTAC 
AGAATCACTG GAAATACTGT GCTGTGTTAC TGCTGTGGTC TGCGTAGCTT CCGAGAGCTG 
ACCTACCAGT ATCGTCAGAA CATTCCTGCT TCTGAGTTGC CAGTGACTGT AACATCCCGT 
CCTGACTGCT ACTGGGGCCG TAACTGTCGC ACTCAGGTGA AGGCTCACCA TGCAATGAAA 
TTCAATCACA TCTGTGAGCA AACAAGGTTC AAGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001276507.1
CDS	117..1895
Translation	MELHGEEQPPPPQEPWGRLLRLGAEEDEPQILLWKREWTIGRRRGCDLSFPSNKLVSGDHCKLTVDEISGEVTLEDTSTNGTVINKLQVVKKQTYPLQSGDIIYLVYRKNEPEHNVAYLYESLSGKQSLTQDSLGPGNTSISPKGRSSLVANGELSSLSPVFQDKEASFSLLESKDHEELEPAKKKMKGDGELDTNLQLLVSGQRGNAQTSSEDVKDASVKPDKMEETLTCIICQDLLHDCVSLQPCMHTFCAACYSGWMERSSLCPTCRCPVERICKNHILNNLVEAYLIQHPDKSRSEEDVRSMDARNKITQDMLQPKVRRSFSDEEGSSEDLLELSDVDSESSDISQPYIVCRQCPEYRRQAVQSLPCPVPESELGATLALGGEAPSTSASLPTAAPDYMCPLQGSHAICTCCFQPMPDRRAEREQDPRVAPQQCAVCLQPFCHLYWGCTRTGCFGCLAPFCELNLGDKCLDGVLNNNNYESDILKNYLATRGLTWKSVLTESLLALQRGVFMLSDYRITGNTVLCYCCGLRSFRELTYQYRQNIPASELPVTVTSRPDCYWGRNCRTQVKAHHAMKFNHICEQTRFKN

Target ORF information:

RefSeq Version	NM_001289578.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus checkpoint with forkhead and ring finger domains (Chfr), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001289578.1

ORF Insert Sequence:

ATGGAGCTAC ACGGGGAAGA GCAGCCGCCG CCGCCGCAGG AACCCTGGGG GAGGCTTCTT 
CGTCTAGGCG CAGAGGAGGA CGAACCCCAG ATCCTTCTTT GGAAACGCGA GTGGACCATC 
GGAAGGAGGA GAGGCTGTGA CCTCTCTTTC CCCAGCAATA AACTGGTCTC TGGAGATCAC 
TGTAAACTTA CAGTGGATGA AATATCTGGT GAGGTGACAC TGGAAGACAC CAGCACCAAT 
GGAACAGTGA TCAATAAGCT GCAAGTTGTT AAGAAGCAGA CTTACCCTTT ACAGAGCGGG 
GATATCATCT ATTTGGTGTA CAGGAAGAAT GAGCCAGAAC ACAATGTGGC ATACCTCTAT 
GAATCTTTAA GTGGCAAACA GAGCTTAACT CAAGACTCCC TTGGACCTGG GAACACAAGC 
ATCTCCCCAA AAGGACGCAG TTCACTTGTT GCAAATGGCG AACTCTCTAG CCTTTCTCCA 
GTTTTCCAAG ACAAAGAAGC ATCCTTTTCT TTGCTGGAAA GTAAAGACCA TGAGGAATTG 
GAGCCTGCCA AAAAAAAGAT GAAAGGAGAT GGGGAACTTG ACACGAACCT CCAGTTATTA 
GTTTCAGGCC AGCGTGGAAA TGCCCAAACC TCAAGTGAAG ATGTCAAAGA TGCCTCTGTG 
AAGCCAGACA AGATGGAGGA GACACTAACC TGTATCATCT GCCAGGACCT TCTGCACGAT 
TGTGTGAGTT TGCAGCCTTG TATGCACACA TTTTGTGCGG CTTGCTACTC TGGTTGGATG 
GAGCGTTCAT CTCTGTGCCC TACCTGCCGA TGTCCAGTGG AGCGGATTTG CAAAAACCAC 
ATCCTGAACA ACCTAGTGGA AGCATACCTT ATCCAGCACC CAGATAAAAG TCGCAGTGAA 
GAAGATGTGA GAAGTATGGA TGCAAGGAAT AAAATCACTC AAGATATGCT GCAACCCAAA 
GTCAGGAGGT CTTTCTCTGA TGAAGAGGGG AGTTCAGAGG ACCTGCTAGA GCTGTCTGAT 
GTCGACAGTG AATCCTCAGA TATCAGTCAG CCATACATTG TCTGCAGACA GTGTCCTGAA 
TACAGAAGGC AAGCGGTGCA GTCTCTTCCT TGCCCAGTCC CAGAGAGTGA GCTGGGAGCT 
ACACTGGCCC TTGGTGGGGA GGCACCTTCA ACATCTGCCA GCTTGCCAAC AGCAGCCCCG 
GATTACATGT GCCCTCTTCA AGGAAGCCAT GCCATATGCA CCTGCTGCTT CCAGCCTATG 
CCTGACCGGA GAGCTGAACG GGAGCAGGAT CCCCGCGTCG CCCCTCAGCA GTGTGCGGTG 
TGCCTGCAGC CCTTCTGCCA CCTGTACTGG GGCTGCACGA GGACTGGCTG CTTTGGCTGC 
TTGGCCCCAT TCTGTGAGCT CAACCTGGGG GACAAGTGCT TGGATGGAGT GCTGAACAAT 
AACAATTATG AATCGGACAT CCTGAAGAAT TACCTGGCAA CCAGGGGTCT GACATGGAAA 
AGTGTGTTGA CAGAGAGTCT CCTGGCTCTG CAGCGAGGTG TATTTATGCT GTCTGATTAC 
AGAATCACTG GAAATACTGT GCTGTGTTAC TGCTGTGGTC TGCGTAGCTT CCGAGAGCTG 
ACCTACCAGT ATCGTCAGAA CATTCCTGCT TCTGAGTTGC CAGTGACTGT AACATCCCGT 
CCTGACTGCT ACTGGGGCCG TAACTGTCGC ACTCAGGTGA AGGCTCACCA TGCAATGAAA 
TTCAATCACA TCTGTGAGCA AACAAGGTTC AAGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chfr ( NM_001289580.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001276509.1 Mus musculus checkpoint with forkhead and ring finger domains (Chfr), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OMu16462
Clone ID Related Accession (Same CDS sequence)	NM_001289580.1 , XM_030254419.1 , XM_030254418.1
Accession Version	NM_001289580.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1572bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase CHFR isoform 5
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC118260.7, AK048930.1 and AK169182.1. Transcript Variant: This variant (5) contains an alternate exon in the 5' region, uses an alternate in-frame splice site, and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (5) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK048930.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849384 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTCCATG TGACCAAAGA TTGCTCAGGT CCAGGGCAGG GTGATGATCC CCAGGTTCCA 
CTATTGTCAC CCATGGCTCA GACATGCTTA GAGGAACCAC AGCCATCAAC ATCGACATCA 
GACCTCCTCC CCACGGCCTC TACCTCTTCT ACGGAGCCAG AGCTGACCTC TGCAGGGCAA 
AAGCATTCTT CTAGCTCTGG ACCTGGGAAC ACAAGCATCT CCCCAAAAGG ACGCAGTTCA 
CTTGTTGCAA ATGGCGAACT CTCTAGCCTT TCTCCAGTTT TCCAAGACAA AGAAGCATCC 
TTTTCTTTGC TGGAAAGTAA AGACCATGAG GAATTGGAGC CTGCCAAAAA AAAGATGAAA 
GGAGATGGGG AACTTGACAC GAACCTCCAG TTATTAGTTT CAGGCCAGCG TGGAAATGCC 
CAAACCTCAA GTGAAGATGT CAAAGATGCC TCTGTGAAGC CAGACAAGAT GGAGGAGACA 
CTAACCTGTA TCATCTGCCA GGACCTTCTG CACGATTGTG TGAGTTTGCA GCCTTGTATG 
CACACATTTT GTGCGGCTTG CTACTCTGGT TGGATGGAGC GTTCATCTCT GTGCCCTACC 
TGCCGATGTC CAGTGGAGCG GATTTGCAAA AACCACATCC TGAACAACCT AGTGGAAGCA 
TACCTTATCC AGCACCCAGA TAAAAGTCGC AGTGAAGAAG ATGTGAGAAG TATGGATGCA 
AGGAATAAAA TCACTCAAGA TATGCTGCAA CCCAAAGTCA GGAGGTCTTT CTCTGATGAA 
GAGGGGAGTT CAGAGGACCT GCTAGAGCTG TCTGATGTCG ACAGTGAATC CTCAGATATC 
AGTCAGCCAT ACATTGTCTG CAGACAGTGT CCTGAATACA GAAGGCAAGC GGTGCAGTCT 
CTTCCTTGCC CAGTCCCAGA GAGTGAGCTG GGAGCTACAC TGGCCCTTGG TGGGGAGGCA 
CCTTCAACAT CTGCCAGCTT GCCAACAGCC CCGGATTACA TGTGCCCTCT TCAAGGAAGC 
CATGCCATAT GCACCTGCTG CTTCCAGCCT ATGCCTGACC GGAGAGCTGA ACGGGAGCAG 
GATCCCCGCG TCGCCCCTCA GCAGTGTGCG GTGTGCCTGC AGCCCTTCTG CCACCTGTAC 
TGGGGCTGCA CGAGGACTGG CTGCTTTGGC TGCTTGGCCC CATTCTGTGA GCTCAACCTG 
GGGGACAAGT GCTTGGATGG AGTGCTGAAC AATAACAATT ATGAATCGGA CATCCTGAAG 
AATTACCTGG CAACCAGGGG TCTGACATGG AAAAGTGTGT TGACAGAGAG TCTCCTGGCT 
CTGCAGCGAG GTGTATTTAT GCTGTCTGAT TACAGAATCA CTGGAAATAC TGTGCTGTGT 
TACTGCTGTG GTCTGCGTAG CTTCCGAGAG CTGACCTACC AGTATCGTCA GAACATTCCT 
GCTTCTGAGT TGCCAGTGAC TGTAACATCC CGTCCTGACT GCTACTGGGG CCGTAACTGT 
CGCACTCAGG TGAAGGCTCA CCATGCAATG AAATTCAATC ACATCTGTGA GCAAACAAGG 
TTCAAGAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001276509.1
CDS	707..2278
Translation	MFHVTKDCSGPGQGDDPQVPLLSPMAQTCLEEPQPSTSTSDLLPTASTSSTEPELTSAGQKHSSSSGPGNTSISPKGRSSLVANGELSSLSPVFQDKEASFSLLESKDHEELEPAKKKMKGDGELDTNLQLLVSGQRGNAQTSSEDVKDASVKPDKMEETLTCIICQDLLHDCVSLQPCMHTFCAACYSGWMERSSLCPTCRCPVERICKNHILNNLVEAYLIQHPDKSRSEEDVRSMDARNKITQDMLQPKVRRSFSDEEGSSEDLLELSDVDSESSDISQPYIVCRQCPEYRRQAVQSLPCPVPESELGATLALGGEAPSTSASLPTAPDYMCPLQGSHAICTCCFQPMPDRRAEREQDPRVAPQQCAVCLQPFCHLYWGCTRTGCFGCLAPFCELNLGDKCLDGVLNNNNYESDILKNYLATRGLTWKSVLTESLLALQRGVFMLSDYRITGNTVLCYCCGLRSFRELTYQYRQNIPASELPVTVTSRPDCYWGRNCRTQVKAHHAMKFNHICEQTRFKN

Target ORF information:

RefSeq Version	NM_001289580.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus checkpoint with forkhead and ring finger domains (Chfr), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001289580.1

ORF Insert Sequence:

ATGTTCCATG TGACCAAAGA TTGCTCAGGT CCAGGGCAGG GTGATGATCC CCAGGTTCCA 
CTATTGTCAC CCATGGCTCA GACATGCTTA GAGGAACCAC AGCCATCAAC ATCGACATCA 
GACCTCCTCC CCACGGCCTC TACCTCTTCT ACGGAGCCAG AGCTGACCTC TGCAGGGCAA 
AAGCATTCTT CTAGCTCTGG ACCTGGGAAC ACAAGCATCT CCCCAAAAGG ACGCAGTTCA 
CTTGTTGCAA ATGGCGAACT CTCTAGCCTT TCTCCAGTTT TCCAAGACAA AGAAGCATCC 
TTTTCTTTGC TGGAAAGTAA AGACCATGAG GAATTGGAGC CTGCCAAAAA AAAGATGAAA 
GGAGATGGGG AACTTGACAC GAACCTCCAG TTATTAGTTT CAGGCCAGCG TGGAAATGCC 
CAAACCTCAA GTGAAGATGT CAAAGATGCC TCTGTGAAGC CAGACAAGAT GGAGGAGACA 
CTAACCTGTA TCATCTGCCA GGACCTTCTG CACGATTGTG TGAGTTTGCA GCCTTGTATG 
CACACATTTT GTGCGGCTTG CTACTCTGGT TGGATGGAGC GTTCATCTCT GTGCCCTACC 
TGCCGATGTC CAGTGGAGCG GATTTGCAAA AACCACATCC TGAACAACCT AGTGGAAGCA 
TACCTTATCC AGCACCCAGA TAAAAGTCGC AGTGAAGAAG ATGTGAGAAG TATGGATGCA 
AGGAATAAAA TCACTCAAGA TATGCTGCAA CCCAAAGTCA GGAGGTCTTT CTCTGATGAA 
GAGGGGAGTT CAGAGGACCT GCTAGAGCTG TCTGATGTCG ACAGTGAATC CTCAGATATC 
AGTCAGCCAT ACATTGTCTG CAGACAGTGT CCTGAATACA GAAGGCAAGC GGTGCAGTCT 
CTTCCTTGCC CAGTCCCAGA GAGTGAGCTG GGAGCTACAC TGGCCCTTGG TGGGGAGGCA 
CCTTCAACAT CTGCCAGCTT GCCAACAGCC CCGGATTACA TGTGCCCTCT TCAAGGAAGC 
CATGCCATAT GCACCTGCTG CTTCCAGCCT ATGCCTGACC GGAGAGCTGA ACGGGAGCAG 
GATCCCCGCG TCGCCCCTCA GCAGTGTGCG GTGTGCCTGC AGCCCTTCTG CCACCTGTAC 
TGGGGCTGCA CGAGGACTGG CTGCTTTGGC TGCTTGGCCC CATTCTGTGA GCTCAACCTG 
GGGGACAAGT GCTTGGATGG AGTGCTGAAC AATAACAATT ATGAATCGGA CATCCTGAAG 
AATTACCTGG CAACCAGGGG TCTGACATGG AAAAGTGTGT TGACAGAGAG TCTCCTGGCT 
CTGCAGCGAG GTGTATTTAT GCTGTCTGAT TACAGAATCA CTGGAAATAC TGTGCTGTGT 
TACTGCTGTG GTCTGCGTAG CTTCCGAGAG CTGACCTACC AGTATCGTCA GAACATTCCT 
GCTTCTGAGT TGCCAGTGAC TGTAACATCC CGTCCTGACT GCTACTGGGG CCGTAACTGT 
CGCACTCAGG TGAAGGCTCA CCATGCAATG AAATTCAATC ACATCTGTGA GCAAACAAGG 
TTCAAGAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chfr ( NM_172717.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_766305.2 Mus musculus checkpoint with forkhead and ring finger domains (Chfr), transcript variant 2, mRNA.

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CloneID	OMu16505
Clone ID Related Accession (Same CDS sequence)	NM_172717.4
Accession Version	NM_172717.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1992bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase CHFR isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC118260.7 and AK077629.1. On Jan 24, 2014 this sequence version replaced NM_172717.3. Transcript Variant: This variant (2) uses an alternate in-frame splice site, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK155295.1, AK155525.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCTAC ACGGGGAAGA GCAGCCGCCG CCGCCGCAGG AACCCTGGGG GAGGCTTCTT 
CGTCTAGGCG CAGAGGAGGA CGAACCCCAG ATCCTTCTTT GGAAACGCGA GTGGACCATC 
GGAAGGAGGA GAGGCTGTGA CCTCTCTTTC CCCAGCAATA AACTGGTCTC TGGAGATCAC 
TGTAAACTTA CAGTGGATGA AATATCTGGT GAGGTGACAC TGGAAGACAC CAGCACCAAT 
GGAACAGTGA TCAATAAGCT GCAAGTTGTT AAGAAGCAGA CTTACCCTTT ACAGAGCGGG 
GATATCATCT ATTTGGTGTA CAGGAAGAAT GAGCCAGAAC ACAATGTGGC ATACCTCTAT 
GAATCTTTAA GTGGCAAACA GAGCTTAACT CAAGACTCCC TTGAAGCCAA TAAGGAAAAT 
ATGTTCCATG TGACCAAAGA TTGCTCAGGT CCAGGGCAGG GTGATGATCC CCAGGTTCCA 
CTATTGTCAC CCATGGCTCA GACATGCTTA GAGGAACCAC AGCCATCAAC ATCGACATCA 
GACCTCCTCC CCACGGCCTC TACCTCTTCT ACGGAGCCAG AGCTGACCTC TGCAGGGCAA 
AAGCATTCTT CTAGCTCTGG ACCTGGGAAC ACAAGCATCT CCCCAAAAGG ACGCAGTTCA 
CTTGTTGCAA ATGGCGAACT CTCTAGCCTT TCTCCAGTTT TCCAAGACAA AGAAGCATCC 
TTTTCTTTGC TGGAAAGTAA AGACCATGAG GAATTGGAGC CTGCCAAAAA AAAGATGAAA 
GGAGATGGGG AACTTGACAC GAACCTCCAG TTATTAGTTT CAGGCCAGCG TGGAAATGCC 
CAAACCTCAA GTGAAGATGT CAAAGATGCC TCTGTGAAGC CAGACAAGAT GGAGGAGACA 
CTAACCTGTA TCATCTGCCA GGACCTTCTG CACGATTGTG TGAGTTTGCA GCCTTGTATG 
CACACATTTT GTGCGGCTTG CTACTCTGGT TGGATGGAGC GTTCATCTCT GTGCCCTACC 
TGCCGATGTC CAGTGGAGCG GATTTGCAAA AACCACATCC TGAACAACCT AGTGGAAGCA 
TACCTTATCC AGCACCCAGA TAAAAGTCGC AGTGAAGAAG ATGTGAGAAG TATGGATGCA 
AGGAATAAAA TCACTCAAGA TATGCTGCAA CCCAAAGTCA GGAGGTCTTT CTCTGATGAA 
GAGGGGAGTT CAGAGGACCT GCTAGAGCTG TCTGATGTCG ACAGTGAATC CTCAGATATC 
AGTCAGCCAT ACATTGTCTG CAGACAGTGT CCTGAATACA GAAGGCAAGC GGTGCAGTCT 
CTTCCTTGCC CAGTCCCAGA GAGTGAGCTG GGAGCTACAC TGGCCCTTGG TGGGGAGGCA 
CCTTCAACAT CTGCCAGCTT GCCAACAGCC CCGGATTACA TGTGCCCTCT TCAAGGAAGC 
CATGCCATAT GCACCTGCTG CTTCCAGCCT ATGCCTGACC GGAGAGCTGA ACGGGAGCAG 
GATCCCCGCG TCGCCCCTCA GCAGTGTGCG GTGTGCCTGC AGCCCTTCTG CCACCTGTAC 
TGGGGCTGCA CGAGGACTGG CTGCTTTGGC TGCTTGGCCC CATTCTGTGA GCTCAACCTG 
GGGGACAAGT GCTTGGATGG AGTGCTGAAC AATAACAATT ATGAATCGGA CATCCTGAAG 
AATTACCTGG CAACCAGGGG TCTGACATGG AAAAGTGTGT TGACAGAGAG TCTCCTGGCT 
CTGCAGCGAG GTGTATTTAT GCTGTCTGAT TACAGAATCA CTGGAAATAC TGTGCTGTGT 
TACTGCTGTG GTCTGCGTAG CTTCCGAGAG CTGACCTACC AGTATCGTCA GAACATTCCT 
GCTTCTGAGT TGCCAGTGAC TGTAACATCC CGTCCTGACT GCTACTGGGG CCGTAACTGT 
CGCACTCAGG TGAAGGCTCA CCATGCAATG AAATTCAATC ACATCTGTGA GCAAACAAGG 
TTCAAGAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_766305.2
CDS	117..2108
Translation	MELHGEEQPPPPQEPWGRLLRLGAEEDEPQILLWKREWTIGRRRGCDLSFPSNKLVSGDHCKLTVDEISGEVTLEDTSTNGTVINKLQVVKKQTYPLQSGDIIYLVYRKNEPEHNVAYLYESLSGKQSLTQDSLEANKENMFHVTKDCSGPGQGDDPQVPLLSPMAQTCLEEPQPSTSTSDLLPTASTSSTEPELTSAGQKHSSSSGPGNTSISPKGRSSLVANGELSSLSPVFQDKEASFSLLESKDHEELEPAKKKMKGDGELDTNLQLLVSGQRGNAQTSSEDVKDASVKPDKMEETLTCIICQDLLHDCVSLQPCMHTFCAACYSGWMERSSLCPTCRCPVERICKNHILNNLVEAYLIQHPDKSRSEEDVRSMDARNKITQDMLQPKVRRSFSDEEGSSEDLLELSDVDSESSDISQPYIVCRQCPEYRRQAVQSLPCPVPESELGATLALGGEAPSTSASLPTAPDYMCPLQGSHAICTCCFQPMPDRRAEREQDPRVAPQQCAVCLQPFCHLYWGCTRTGCFGCLAPFCELNLGDKCLDGVLNNNNYESDILKNYLATRGLTWKSVLTESLLALQRGVFMLSDYRITGNTVLCYCCGLRSFRELTYQYRQNIPASELPVTVTSRPDCYWGRNCRTQVKAHHAMKFNHICEQTRFKN

Target ORF information:

RefSeq Version	NM_172717.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus checkpoint with forkhead and ring finger domains (Chfr), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_172717.4

ORF Insert Sequence:

ATGGAGCTAC ACGGGGAAGA GCAGCCGCCG CCGCCGCAGG AACCCTGGGG GAGGCTTCTT 
CGTCTAGGCG CAGAGGAGGA CGAACCCCAG ATCCTTCTTT GGAAACGCGA GTGGACCATC 
GGAAGGAGGA GAGGCTGTGA CCTCTCTTTC CCCAGCAATA AACTGGTCTC TGGAGATCAC 
TGTAAACTTA CAGTGGATGA AATATCTGGT GAGGTGACAC TGGAAGACAC CAGCACCAAT 
GGAACAGTGA TCAATAAGCT GCAAGTTGTT AAGAAGCAGA CTTACCCTTT ACAGAGCGGG 
GATATCATCT ATTTGGTGTA CAGGAAGAAT GAGCCAGAAC ACAATGTGGC ATACCTCTAT 
GAATCTTTAA GTGGCAAACA GAGCTTAACT CAAGACTCCC TTGAAGCCAA TAAGGAAAAT 
ATGTTCCATG TGACCAAAGA TTGCTCAGGT CCAGGGCAGG GTGATGATCC CCAGGTTCCA 
CTATTGTCAC CCATGGCTCA GACATGCTTA GAGGAACCAC AGCCATCAAC ATCGACATCA 
GACCTCCTCC CCACGGCCTC TACCTCTTCT ACGGAGCCAG AGCTGACCTC TGCAGGGCAA 
AAGCATTCTT CTAGCTCTGG ACCTGGGAAC ACAAGCATCT CCCCAAAAGG ACGCAGTTCA 
CTTGTTGCAA ATGGCGAACT CTCTAGCCTT TCTCCAGTTT TCCAAGACAA AGAAGCATCC 
TTTTCTTTGC TGGAAAGTAA AGACCATGAG GAATTGGAGC CTGCCAAAAA AAAGATGAAA 
GGAGATGGGG AACTTGACAC GAACCTCCAG TTATTAGTTT CAGGCCAGCG TGGAAATGCC 
CAAACCTCAA GTGAAGATGT CAAAGATGCC TCTGTGAAGC CAGACAAGAT GGAGGAGACA 
CTAACCTGTA TCATCTGCCA GGACCTTCTG CACGATTGTG TGAGTTTGCA GCCTTGTATG 
CACACATTTT GTGCGGCTTG CTACTCTGGT TGGATGGAGC GTTCATCTCT GTGCCCTACC 
TGCCGATGTC CAGTGGAGCG GATTTGCAAA AACCACATCC TGAACAACCT AGTGGAAGCA 
TACCTTATCC AGCACCCAGA TAAAAGTCGC AGTGAAGAAG ATGTGAGAAG TATGGATGCA 
AGGAATAAAA TCACTCAAGA TATGCTGCAA CCCAAAGTCA GGAGGTCTTT CTCTGATGAA 
GAGGGGAGTT CAGAGGACCT GCTAGAGCTG TCTGATGTCG ACAGTGAATC CTCAGATATC 
AGTCAGCCAT ACATTGTCTG CAGACAGTGT CCTGAATACA GAAGGCAAGC GGTGCAGTCT 
CTTCCTTGCC CAGTCCCAGA GAGTGAGCTG GGAGCTACAC TGGCCCTTGG TGGGGAGGCA 
CCTTCAACAT CTGCCAGCTT GCCAACAGCC CCGGATTACA TGTGCCCTCT TCAAGGAAGC 
CATGCCATAT GCACCTGCTG CTTCCAGCCT ATGCCTGACC GGAGAGCTGA ACGGGAGCAG 
GATCCCCGCG TCGCCCCTCA GCAGTGTGCG GTGTGCCTGC AGCCCTTCTG CCACCTGTAC 
TGGGGCTGCA CGAGGACTGG CTGCTTTGGC TGCTTGGCCC CATTCTGTGA GCTCAACCTG 
GGGGACAAGT GCTTGGATGG AGTGCTGAAC AATAACAATT ATGAATCGGA CATCCTGAAG 
AATTACCTGG CAACCAGGGG TCTGACATGG AAAAGTGTGT TGACAGAGAG TCTCCTGGCT 
CTGCAGCGAG GTGTATTTAT GCTGTCTGAT TACAGAATCA CTGGAAATAC TGTGCTGTGT 
TACTGCTGTG GTCTGCGTAG CTTCCGAGAG CTGACCTACC AGTATCGTCA GAACATTCCT 
GCTTCTGAGT TGCCAGTGAC TGTAACATCC CGTCCTGACT GCTACTGGGG CCGTAACTGT 
CGCACTCAGG TGAAGGCTCA CCATGCAATG AAATTCAATC ACATCTGTGA GCAAACAAGG 
TTCAAGAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chfr ( NM_001289577.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001276506.1 Mus musculus checkpoint with forkhead and ring finger domains (Chfr), transcript variant 1, mRNA.

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CloneID	OMu17144
Clone ID Related Accession (Same CDS sequence)	NM_001289577.1
Accession Version	NM_001289577.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1995bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase CHFR isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC118260.7. On Jan 24, 2014 this sequence version replaced XM_006534914.1. Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AK154038.1, SRR1660811.16989.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCTAC ACGGGGAAGA GCAGCCGCCG CCGCCGCAGG AACCCTGGGG GAGGCTTCTT 
CGTCTAGGCG CAGAGGAGGA CGAACCCCAG ATCCTTCTTT GGAAACGCGA GTGGACCATC 
GGAAGGAGGA GAGGCTGTGA CCTCTCTTTC CCCAGCAATA AACTGGTCTC TGGAGATCAC 
TGTAAACTTA CAGTGGATGA AATATCTGGT GAGGTGACAC TGGAAGACAC CAGCACCAAT 
GGAACAGTGA TCAATAAGCT GCAAGTTGTT AAGAAGCAGA CTTACCCTTT ACAGAGCGGG 
GATATCATCT ATTTGGTGTA CAGGAAGAAT GAGCCAGAAC ACAATGTGGC ATACCTCTAT 
GAATCTTTAA GTGGCAAACA GAGCTTAACT CAAGACTCCC TTGAAGCCAA TAAGGAAAAT 
ATGTTCCATG TGACCAAAGA TTGCTCAGGT CCAGGGCAGG GTGATGATCC CCAGGTTCCA 
CTATTGTCAC CCATGGCTCA GACATGCTTA GAGGAACCAC AGCCATCAAC ATCGACATCA 
GACCTCCTCC CCACGGCCTC TACCTCTTCT ACGGAGCCAG AGCTGACCTC TGCAGGGCAA 
AAGCATTCTT CTAGCTCTGG ACCTGGGAAC ACAAGCATCT CCCCAAAAGG ACGCAGTTCA 
CTTGTTGCAA ATGGCGAACT CTCTAGCCTT TCTCCAGTTT TCCAAGACAA AGAAGCATCC 
TTTTCTTTGC TGGAAAGTAA AGACCATGAG GAATTGGAGC CTGCCAAAAA AAAGATGAAA 
GGAGATGGGG AACTTGACAC GAACCTCCAG TTATTAGTTT CAGGCCAGCG TGGAAATGCC 
CAAACCTCAA GTGAAGATGT CAAAGATGCC TCTGTGAAGC CAGACAAGAT GGAGGAGACA 
CTAACCTGTA TCATCTGCCA GGACCTTCTG CACGATTGTG TGAGTTTGCA GCCTTGTATG 
CACACATTTT GTGCGGCTTG CTACTCTGGT TGGATGGAGC GTTCATCTCT GTGCCCTACC 
TGCCGATGTC CAGTGGAGCG GATTTGCAAA AACCACATCC TGAACAACCT AGTGGAAGCA 
TACCTTATCC AGCACCCAGA TAAAAGTCGC AGTGAAGAAG ATGTGAGAAG TATGGATGCA 
AGGAATAAAA TCACTCAAGA TATGCTGCAA CCCAAAGTCA GGAGGTCTTT CTCTGATGAA 
GAGGGGAGTT CAGAGGACCT GCTAGAGCTG TCTGATGTCG ACAGTGAATC CTCAGATATC 
AGTCAGCCAT ACATTGTCTG CAGACAGTGT CCTGAATACA GAAGGCAAGC GGTGCAGTCT 
CTTCCTTGCC CAGTCCCAGA GAGTGAGCTG GGAGCTACAC TGGCCCTTGG TGGGGAGGCA 
CCTTCAACAT CTGCCAGCTT GCCAACAGCA GCCCCGGATT ACATGTGCCC TCTTCAAGGA 
AGCCATGCCA TATGCACCTG CTGCTTCCAG CCTATGCCTG ACCGGAGAGC TGAACGGGAG 
CAGGATCCCC GCGTCGCCCC TCAGCAGTGT GCGGTGTGCC TGCAGCCCTT CTGCCACCTG 
TACTGGGGCT GCACGAGGAC TGGCTGCTTT GGCTGCTTGG CCCCATTCTG TGAGCTCAAC 
CTGGGGGACA AGTGCTTGGA TGGAGTGCTG AACAATAACA ATTATGAATC GGACATCCTG 
AAGAATTACC TGGCAACCAG GGGTCTGACA TGGAAAAGTG TGTTGACAGA GAGTCTCCTG 
GCTCTGCAGC GAGGTGTATT TATGCTGTCT GATTACAGAA TCACTGGAAA TACTGTGCTG 
TGTTACTGCT GTGGTCTGCG TAGCTTCCGA GAGCTGACCT ACCAGTATCG TCAGAACATT 
CCTGCTTCTG AGTTGCCAGT GACTGTAACA TCCCGTCCTG ACTGCTACTG GGGCCGTAAC 
TGTCGCACTC AGGTGAAGGC TCACCATGCA ATGAAATTCA ATCACATCTG TGAGCAAACA 
AGGTTCAAGA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001276506.1
CDS	117..2111
Translation	MELHGEEQPPPPQEPWGRLLRLGAEEDEPQILLWKREWTIGRRRGCDLSFPSNKLVSGDHCKLTVDEISGEVTLEDTSTNGTVINKLQVVKKQTYPLQSGDIIYLVYRKNEPEHNVAYLYESLSGKQSLTQDSLEANKENMFHVTKDCSGPGQGDDPQVPLLSPMAQTCLEEPQPSTSTSDLLPTASTSSTEPELTSAGQKHSSSSGPGNTSISPKGRSSLVANGELSSLSPVFQDKEASFSLLESKDHEELEPAKKKMKGDGELDTNLQLLVSGQRGNAQTSSEDVKDASVKPDKMEETLTCIICQDLLHDCVSLQPCMHTFCAACYSGWMERSSLCPTCRCPVERICKNHILNNLVEAYLIQHPDKSRSEEDVRSMDARNKITQDMLQPKVRRSFSDEEGSSEDLLELSDVDSESSDISQPYIVCRQCPEYRRQAVQSLPCPVPESELGATLALGGEAPSTSASLPTAAPDYMCPLQGSHAICTCCFQPMPDRRAEREQDPRVAPQQCAVCLQPFCHLYWGCTRTGCFGCLAPFCELNLGDKCLDGVLNNNNYESDILKNYLATRGLTWKSVLTESLLALQRGVFMLSDYRITGNTVLCYCCGLRSFRELTYQYRQNIPASELPVTVTSRPDCYWGRNCRTQVKAHHAMKFNHICEQTRFKN

Target ORF information:

RefSeq Version	NM_001289577.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus checkpoint with forkhead and ring finger domains (Chfr), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001289577.1

ORF Insert Sequence:

ATGGAGCTAC ACGGGGAAGA GCAGCCGCCG CCGCCGCAGG AACCCTGGGG GAGGCTTCTT 
CGTCTAGGCG CAGAGGAGGA CGAACCCCAG ATCCTTCTTT GGAAACGCGA GTGGACCATC 
GGAAGGAGGA GAGGCTGTGA CCTCTCTTTC CCCAGCAATA AACTGGTCTC TGGAGATCAC 
TGTAAACTTA CAGTGGATGA AATATCTGGT GAGGTGACAC TGGAAGACAC CAGCACCAAT 
GGAACAGTGA TCAATAAGCT GCAAGTTGTT AAGAAGCAGA CTTACCCTTT ACAGAGCGGG 
GATATCATCT ATTTGGTGTA CAGGAAGAAT GAGCCAGAAC ACAATGTGGC ATACCTCTAT 
GAATCTTTAA GTGGCAAACA GAGCTTAACT CAAGACTCCC TTGAAGCCAA TAAGGAAAAT 
ATGTTCCATG TGACCAAAGA TTGCTCAGGT CCAGGGCAGG GTGATGATCC CCAGGTTCCA 
CTATTGTCAC CCATGGCTCA GACATGCTTA GAGGAACCAC AGCCATCAAC ATCGACATCA 
GACCTCCTCC CCACGGCCTC TACCTCTTCT ACGGAGCCAG AGCTGACCTC TGCAGGGCAA 
AAGCATTCTT CTAGCTCTGG ACCTGGGAAC ACAAGCATCT CCCCAAAAGG ACGCAGTTCA 
CTTGTTGCAA ATGGCGAACT CTCTAGCCTT TCTCCAGTTT TCCAAGACAA AGAAGCATCC 
TTTTCTTTGC TGGAAAGTAA AGACCATGAG GAATTGGAGC CTGCCAAAAA AAAGATGAAA 
GGAGATGGGG AACTTGACAC GAACCTCCAG TTATTAGTTT CAGGCCAGCG TGGAAATGCC 
CAAACCTCAA GTGAAGATGT CAAAGATGCC TCTGTGAAGC CAGACAAGAT GGAGGAGACA 
CTAACCTGTA TCATCTGCCA GGACCTTCTG CACGATTGTG TGAGTTTGCA GCCTTGTATG 
CACACATTTT GTGCGGCTTG CTACTCTGGT TGGATGGAGC GTTCATCTCT GTGCCCTACC 
TGCCGATGTC CAGTGGAGCG GATTTGCAAA AACCACATCC TGAACAACCT AGTGGAAGCA 
TACCTTATCC AGCACCCAGA TAAAAGTCGC AGTGAAGAAG ATGTGAGAAG TATGGATGCA 
AGGAATAAAA TCACTCAAGA TATGCTGCAA CCCAAAGTCA GGAGGTCTTT CTCTGATGAA 
GAGGGGAGTT CAGAGGACCT GCTAGAGCTG TCTGATGTCG ACAGTGAATC CTCAGATATC 
AGTCAGCCAT ACATTGTCTG CAGACAGTGT CCTGAATACA GAAGGCAAGC GGTGCAGTCT 
CTTCCTTGCC CAGTCCCAGA GAGTGAGCTG GGAGCTACAC TGGCCCTTGG TGGGGAGGCA 
CCTTCAACAT CTGCCAGCTT GCCAACAGCA GCCCCGGATT ACATGTGCCC TCTTCAAGGA 
AGCCATGCCA TATGCACCTG CTGCTTCCAG CCTATGCCTG ACCGGAGAGC TGAACGGGAG 
CAGGATCCCC GCGTCGCCCC TCAGCAGTGT GCGGTGTGCC TGCAGCCCTT CTGCCACCTG 
TACTGGGGCT GCACGAGGAC TGGCTGCTTT GGCTGCTTGG CCCCATTCTG TGAGCTCAAC 
CTGGGGGACA AGTGCTTGGA TGGAGTGCTG AACAATAACA ATTATGAATC GGACATCCTG 
AAGAATTACC TGGCAACCAG GGGTCTGACA TGGAAAAGTG TGTTGACAGA GAGTCTCCTG 
GCTCTGCAGC GAGGTGTATT TATGCTGTCT GATTACAGAA TCACTGGAAA TACTGTGCTG 
TGTTACTGCT GTGGTCTGCG TAGCTTCCGA GAGCTGACCT ACCAGTATCG TCAGAACATT 
CCTGCTTCTG AGTTGCCAGT GACTGTAACA TCCCGTCCTG ACTGCTACTG GGGCCGTAAC 
TGTCGCACTC AGGTGAAGGC TCACCATGCA ATGAAATTCA ATCACATCTG TGAGCAAACA 
AGGTTCAAGA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chfr ( XM_011249446.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011247748.1 Mus musculus checkpoint with forkhead and ring finger domains (Chfr), transcript variant X6, mRNA.

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CloneID	OMu16350
Clone ID Related Accession (Same CDS sequence)	XM_011249446.2 , XM_011249445.2 , NM_001289579.1
Accession Version	XM_011249446.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1575bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase CHFR isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011249446.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCATG TGACCAAAGA TTGCTCAGGT CCAGGGCAGG GTGATGATCC CCAGGTTCCA 
CTATTGTCAC CCATGGCTCA GACATGCTTA GAGGAACCAC AGCCATCAAC ATCGACATCA 
GACCTCCTCC CCACGGCCTC TACCTCTTCT ACGGAGCCAG AGCTGACCTC TGCAGGGCAA 
AAGCATTCTT CTAGCTCTGG ACCTGGGAAC ACAAGCATCT CCCCAAAAGG ACGCAGTTCA 
CTTGTTGCAA ATGGCGAACT CTCTAGCCTT TCTCCAGTTT TCCAAGACAA AGAAGCATCC 
TTTTCTTTGC TGGAAAGTAA AGACCATGAG GAATTGGAGC CTGCCAAAAA AAAGATGAAA 
GGAGATGGGG AACTTGACAC GAACCTCCAG TTATTAGTTT CAGGCCAGCG TGGAAATGCC 
CAAACCTCAA GTGAAGATGT CAAAGATGCC TCTGTGAAGC CAGACAAGAT GGAGGAGACA 
CTAACCTGTA TCATCTGCCA GGACCTTCTG CACGATTGTG TGAGTTTGCA GCCTTGTATG 
CACACATTTT GTGCGGCTTG CTACTCTGGT TGGATGGAGC GTTCATCTCT GTGCCCTACC 
TGCCGATGTC CAGTGGAGCG GATTTGCAAA AACCACATCC TGAACAACCT AGTGGAAGCA 
TACCTTATCC AGCACCCAGA TAAAAGTCGC AGTGAAGAAG ATGTGAGAAG TATGGATGCA 
AGGAATAAAA TCACTCAAGA TATGCTGCAA CCCAAAGTCA GGAGGTCTTT CTCTGATGAA 
GAGGGGAGTT CAGAGGACCT GCTAGAGCTG TCTGATGTCG ACAGTGAATC CTCAGATATC 
AGTCAGCCAT ACATTGTCTG CAGACAGTGT CCTGAATACA GAAGGCAAGC GGTGCAGTCT 
CTTCCTTGCC CAGTCCCAGA GAGTGAGCTG GGAGCTACAC TGGCCCTTGG TGGGGAGGCA 
CCTTCAACAT CTGCCAGCTT GCCAACAGCA GCCCCGGATT ACATGTGCCC TCTTCAAGGA 
AGCCATGCCA TATGCACCTG CTGCTTCCAG CCTATGCCTG ACCGGAGAGC TGAACGGGAG 
CAGGATCCCC GCGTCGCCCC TCAGCAGTGT GCGGTGTGCC TGCAGCCCTT CTGCCACCTG 
TACTGGGGCT GCACGAGGAC TGGCTGCTTT GGCTGCTTGG CCCCATTCTG TGAGCTCAAC 
CTGGGGGACA AGTGCTTGGA TGGAGTGCTG AACAATAACA ATTATGAATC GGACATCCTG 
AAGAATTACC TGGCAACCAG GGGTCTGACA TGGAAAAGTG TGTTGACAGA GAGTCTCCTG 
GCTCTGCAGC GAGGTGTATT TATGCTGTCT GATTACAGAA TCACTGGAAA TACTGTGCTG 
TGTTACTGCT GTGGTCTGCG TAGCTTCCGA GAGCTGACCT ACCAGTATCG TCAGAACATT 
CCTGCTTCTG AGTTGCCAGT GACTGTAACA TCCCGTCCTG ACTGCTACTG GGGCCGTAAC 
TGTCGCACTC AGGTGAAGGC TCACCATGCA ATGAAATTCA ATCACATCTG TGAGCAAACA 
AGGTTCAAGA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011247748.1
CDS	501..2075
Translation	MFHVTKDCSGPGQGDDPQVPLLSPMAQTCLEEPQPSTSTSDLLPTASTSSTEPELTSAGQKHSSSSGPGNTSISPKGRSSLVANGELSSLSPVFQDKEASFSLLESKDHEELEPAKKKMKGDGELDTNLQLLVSGQRGNAQTSSEDVKDASVKPDKMEETLTCIICQDLLHDCVSLQPCMHTFCAACYSGWMERSSLCPTCRCPVERICKNHILNNLVEAYLIQHPDKSRSEEDVRSMDARNKITQDMLQPKVRRSFSDEEGSSEDLLELSDVDSESSDISQPYIVCRQCPEYRRQAVQSLPCPVPESELGATLALGGEAPSTSASLPTAAPDYMCPLQGSHAICTCCFQPMPDRRAEREQDPRVAPQQCAVCLQPFCHLYWGCTRTGCFGCLAPFCELNLGDKCLDGVLNNNNYESDILKNYLATRGLTWKSVLTESLLALQRGVFMLSDYRITGNTVLCYCCGLRSFRELTYQYRQNIPASELPVTVTSRPDCYWGRNCRTQVKAHHAMKFNHICEQTRFKN

Target ORF information:

RefSeq Version	XM_011249446.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus checkpoint with forkhead and ring finger domains (Chfr), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011249446.2

ORF Insert Sequence:

ATGTTCCATG TGACCAAAGA TTGCTCAGGT CCAGGGCAGG GTGATGATCC CCAGGTTCCA 
CTATTGTCAC CCATGGCTCA GACATGCTTA GAGGAACCAC AGCCATCAAC ATCGACATCA 
GACCTCCTCC CCACGGCCTC TACCTCTTCT ACGGAGCCAG AGCTGACCTC TGCAGGGCAA 
AAGCATTCTT CTAGCTCTGG ACCTGGGAAC ACAAGCATCT CCCCAAAAGG ACGCAGTTCA 
CTTGTTGCAA ATGGCGAACT CTCTAGCCTT TCTCCAGTTT TCCAAGACAA AGAAGCATCC 
TTTTCTTTGC TGGAAAGTAA AGACCATGAG GAATTGGAGC CTGCCAAAAA AAAGATGAAA 
GGAGATGGGG AACTTGACAC GAACCTCCAG TTATTAGTTT CAGGCCAGCG TGGAAATGCC 
CAAACCTCAA GTGAAGATGT CAAAGATGCC TCTGTGAAGC CAGACAAGAT GGAGGAGACA 
CTAACCTGTA TCATCTGCCA GGACCTTCTG CACGATTGTG TGAGTTTGCA GCCTTGTATG 
CACACATTTT GTGCGGCTTG CTACTCTGGT TGGATGGAGC GTTCATCTCT GTGCCCTACC 
TGCCGATGTC CAGTGGAGCG GATTTGCAAA AACCACATCC TGAACAACCT AGTGGAAGCA 
TACCTTATCC AGCACCCAGA TAAAAGTCGC AGTGAAGAAG ATGTGAGAAG TATGGATGCA 
AGGAATAAAA TCACTCAAGA TATGCTGCAA CCCAAAGTCA GGAGGTCTTT CTCTGATGAA 
GAGGGGAGTT CAGAGGACCT GCTAGAGCTG TCTGATGTCG ACAGTGAATC CTCAGATATC 
AGTCAGCCAT ACATTGTCTG CAGACAGTGT CCTGAATACA GAAGGCAAGC GGTGCAGTCT 
CTTCCTTGCC CAGTCCCAGA GAGTGAGCTG GGAGCTACAC TGGCCCTTGG TGGGGAGGCA 
CCTTCAACAT CTGCCAGCTT GCCAACAGCA GCCCCGGATT ACATGTGCCC TCTTCAAGGA 
AGCCATGCCA TATGCACCTG CTGCTTCCAG CCTATGCCTG ACCGGAGAGC TGAACGGGAG 
CAGGATCCCC GCGTCGCCCC TCAGCAGTGT GCGGTGTGCC TGCAGCCCTT CTGCCACCTG 
TACTGGGGCT GCACGAGGAC TGGCTGCTTT GGCTGCTTGG CCCCATTCTG TGAGCTCAAC 
CTGGGGGACA AGTGCTTGGA TGGAGTGCTG AACAATAACA ATTATGAATC GGACATCCTG 
AAGAATTACC TGGCAACCAG GGGTCTGACA TGGAAAAGTG TGTTGACAGA GAGTCTCCTG 
GCTCTGCAGC GAGGTGTATT TATGCTGTCT GATTACAGAA TCACTGGAAA TACTGTGCTG 
TGTTACTGCT GTGGTCTGCG TAGCTTCCGA GAGCTGACCT ACCAGTATCG TCAGAACATT 
CCTGCTTCTG AGTTGCCAGT GACTGTAACA TCCCGTCCTG ACTGCTACTG GGGCCGTAAC 
TGTCGCACTC AGGTGAAGGC TCACCATGCA ATGAAATTCA ATCACATCTG TGAGCAAACA 
AGGTTCAAGA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chfr ( XM_011249445.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011247747.1 Mus musculus checkpoint with forkhead and ring finger domains (Chfr), transcript variant X3, mRNA.

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CloneID	OMu16350
Clone ID Related Accession (Same CDS sequence)	XM_011249446.2 , XM_011249445.2 , NM_001289579.1
Accession Version	XM_011249445.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1575bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase CHFR isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011249445.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCATG TGACCAAAGA TTGCTCAGGT CCAGGGCAGG GTGATGATCC CCAGGTTCCA 
CTATTGTCAC CCATGGCTCA GACATGCTTA GAGGAACCAC AGCCATCAAC ATCGACATCA 
GACCTCCTCC CCACGGCCTC TACCTCTTCT ACGGAGCCAG AGCTGACCTC TGCAGGGCAA 
AAGCATTCTT CTAGCTCTGG ACCTGGGAAC ACAAGCATCT CCCCAAAAGG ACGCAGTTCA 
CTTGTTGCAA ATGGCGAACT CTCTAGCCTT TCTCCAGTTT TCCAAGACAA AGAAGCATCC 
TTTTCTTTGC TGGAAAGTAA AGACCATGAG GAATTGGAGC CTGCCAAAAA AAAGATGAAA 
GGAGATGGGG AACTTGACAC GAACCTCCAG TTATTAGTTT CAGGCCAGCG TGGAAATGCC 
CAAACCTCAA GTGAAGATGT CAAAGATGCC TCTGTGAAGC CAGACAAGAT GGAGGAGACA 
CTAACCTGTA TCATCTGCCA GGACCTTCTG CACGATTGTG TGAGTTTGCA GCCTTGTATG 
CACACATTTT GTGCGGCTTG CTACTCTGGT TGGATGGAGC GTTCATCTCT GTGCCCTACC 
TGCCGATGTC CAGTGGAGCG GATTTGCAAA AACCACATCC TGAACAACCT AGTGGAAGCA 
TACCTTATCC AGCACCCAGA TAAAAGTCGC AGTGAAGAAG ATGTGAGAAG TATGGATGCA 
AGGAATAAAA TCACTCAAGA TATGCTGCAA CCCAAAGTCA GGAGGTCTTT CTCTGATGAA 
GAGGGGAGTT CAGAGGACCT GCTAGAGCTG TCTGATGTCG ACAGTGAATC CTCAGATATC 
AGTCAGCCAT ACATTGTCTG CAGACAGTGT CCTGAATACA GAAGGCAAGC GGTGCAGTCT 
CTTCCTTGCC CAGTCCCAGA GAGTGAGCTG GGAGCTACAC TGGCCCTTGG TGGGGAGGCA 
CCTTCAACAT CTGCCAGCTT GCCAACAGCA GCCCCGGATT ACATGTGCCC TCTTCAAGGA 
AGCCATGCCA TATGCACCTG CTGCTTCCAG CCTATGCCTG ACCGGAGAGC TGAACGGGAG 
CAGGATCCCC GCGTCGCCCC TCAGCAGTGT GCGGTGTGCC TGCAGCCCTT CTGCCACCTG 
TACTGGGGCT GCACGAGGAC TGGCTGCTTT GGCTGCTTGG CCCCATTCTG TGAGCTCAAC 
CTGGGGGACA AGTGCTTGGA TGGAGTGCTG AACAATAACA ATTATGAATC GGACATCCTG 
AAGAATTACC TGGCAACCAG GGGTCTGACA TGGAAAAGTG TGTTGACAGA GAGTCTCCTG 
GCTCTGCAGC GAGGTGTATT TATGCTGTCT GATTACAGAA TCACTGGAAA TACTGTGCTG 
TGTTACTGCT GTGGTCTGCG TAGCTTCCGA GAGCTGACCT ACCAGTATCG TCAGAACATT 
CCTGCTTCTG AGTTGCCAGT GACTGTAACA TCCCGTCCTG ACTGCTACTG GGGCCGTAAC 
TGTCGCACTC AGGTGAAGGC TCACCATGCA ATGAAATTCA ATCACATCTG TGAGCAAACA 
AGGTTCAAGA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011247747.1
CDS	579..2153
Translation	MFHVTKDCSGPGQGDDPQVPLLSPMAQTCLEEPQPSTSTSDLLPTASTSSTEPELTSAGQKHSSSSGPGNTSISPKGRSSLVANGELSSLSPVFQDKEASFSLLESKDHEELEPAKKKMKGDGELDTNLQLLVSGQRGNAQTSSEDVKDASVKPDKMEETLTCIICQDLLHDCVSLQPCMHTFCAACYSGWMERSSLCPTCRCPVERICKNHILNNLVEAYLIQHPDKSRSEEDVRSMDARNKITQDMLQPKVRRSFSDEEGSSEDLLELSDVDSESSDISQPYIVCRQCPEYRRQAVQSLPCPVPESELGATLALGGEAPSTSASLPTAAPDYMCPLQGSHAICTCCFQPMPDRRAEREQDPRVAPQQCAVCLQPFCHLYWGCTRTGCFGCLAPFCELNLGDKCLDGVLNNNNYESDILKNYLATRGLTWKSVLTESLLALQRGVFMLSDYRITGNTVLCYCCGLRSFRELTYQYRQNIPASELPVTVTSRPDCYWGRNCRTQVKAHHAMKFNHICEQTRFKN

Target ORF information:

RefSeq Version	XM_011249445.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus checkpoint with forkhead and ring finger domains (Chfr), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011249445.2

ORF Insert Sequence:

ATGTTCCATG TGACCAAAGA TTGCTCAGGT CCAGGGCAGG GTGATGATCC CCAGGTTCCA 
CTATTGTCAC CCATGGCTCA GACATGCTTA GAGGAACCAC AGCCATCAAC ATCGACATCA 
GACCTCCTCC CCACGGCCTC TACCTCTTCT ACGGAGCCAG AGCTGACCTC TGCAGGGCAA 
AAGCATTCTT CTAGCTCTGG ACCTGGGAAC ACAAGCATCT CCCCAAAAGG ACGCAGTTCA 
CTTGTTGCAA ATGGCGAACT CTCTAGCCTT TCTCCAGTTT TCCAAGACAA AGAAGCATCC 
TTTTCTTTGC TGGAAAGTAA AGACCATGAG GAATTGGAGC CTGCCAAAAA AAAGATGAAA 
GGAGATGGGG AACTTGACAC GAACCTCCAG TTATTAGTTT CAGGCCAGCG TGGAAATGCC 
CAAACCTCAA GTGAAGATGT CAAAGATGCC TCTGTGAAGC CAGACAAGAT GGAGGAGACA 
CTAACCTGTA TCATCTGCCA GGACCTTCTG CACGATTGTG TGAGTTTGCA GCCTTGTATG 
CACACATTTT GTGCGGCTTG CTACTCTGGT TGGATGGAGC GTTCATCTCT GTGCCCTACC 
TGCCGATGTC CAGTGGAGCG GATTTGCAAA AACCACATCC TGAACAACCT AGTGGAAGCA 
TACCTTATCC AGCACCCAGA TAAAAGTCGC AGTGAAGAAG ATGTGAGAAG TATGGATGCA 
AGGAATAAAA TCACTCAAGA TATGCTGCAA CCCAAAGTCA GGAGGTCTTT CTCTGATGAA 
GAGGGGAGTT CAGAGGACCT GCTAGAGCTG TCTGATGTCG ACAGTGAATC CTCAGATATC 
AGTCAGCCAT ACATTGTCTG CAGACAGTGT CCTGAATACA GAAGGCAAGC GGTGCAGTCT 
CTTCCTTGCC CAGTCCCAGA GAGTGAGCTG GGAGCTACAC TGGCCCTTGG TGGGGAGGCA 
CCTTCAACAT CTGCCAGCTT GCCAACAGCA GCCCCGGATT ACATGTGCCC TCTTCAAGGA 
AGCCATGCCA TATGCACCTG CTGCTTCCAG CCTATGCCTG ACCGGAGAGC TGAACGGGAG 
CAGGATCCCC GCGTCGCCCC TCAGCAGTGT GCGGTGTGCC TGCAGCCCTT CTGCCACCTG 
TACTGGGGCT GCACGAGGAC TGGCTGCTTT GGCTGCTTGG CCCCATTCTG TGAGCTCAAC 
CTGGGGGACA AGTGCTTGGA TGGAGTGCTG AACAATAACA ATTATGAATC GGACATCCTG 
AAGAATTACC TGGCAACCAG GGGTCTGACA TGGAAAAGTG TGTTGACAGA GAGTCTCCTG 
GCTCTGCAGC GAGGTGTATT TATGCTGTCT GATTACAGAA TCACTGGAAA TACTGTGCTG 
TGTTACTGCT GTGGTCTGCG TAGCTTCCGA GAGCTGACCT ACCAGTATCG TCAGAACATT 
CCTGCTTCTG AGTTGCCAGT GACTGTAACA TCCCGTCCTG ACTGCTACTG GGGCCGTAAC 
TGTCGCACTC AGGTGAAGGC TCACCATGCA ATGAAATTCA ATCACATCTG TGAGCAAACA 
AGGTTCAAGA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chfr ( XM_006534915.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006534978.1 Mus musculus checkpoint with forkhead and ring finger domains (Chfr), transcript variant X1, mRNA.

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CloneID	OMu35453
Clone ID Related Accession (Same CDS sequence)	XM_006534915.1
Accession Version	XM_006534915.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1854bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase CHFR isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATCTC CCAGCAATAA ACTGGTCTCT GGAGATCACT GTAAACTTAC AGTGGATGAA 
ATATCTGGTG AGGTGACACT GGAAGACACC AGCACCAATG GAACAGTGAT CAATAAGCTG 
CAAGTTGTTA AGAAGCAGAC TTACCCTTTA CAGAGCGGGG ATATCATCTA TTTGGTGTAC 
AGGAAGAATG AGCCAGAACA CAATGTGGCA TACCTCTATG AATCTTTAAG TGGCAAACAG 
AGCTTAACTC AAGACTCCCT TGAAGCCAAT AAGGAAAATA TGTTCCATGT GACCAAAGAT 
TGCTCAGGTC CAGGGCAGGG TGATGATCCC CAGGTTCCAC TATTGTCACC CATGGCTCAG 
ACATGCTTAG AGGAACCACA GCCATCAACA TCGACATCAG ACCTCCTCCC CACGGCCTCT 
ACCTCTTCTA CGGAGCCAGA GCTGACCTCT GCAGGGCAAA AGCATTCTTC TAGCTCTGGA 
CCTGGGAACA CAAGCATCTC CCCAAAAGGA CGCAGTTCAC TTGTTGCAAA TGGCGAACTC 
TCTAGCCTTT CTCCAGTTTT CCAAGACAAA GAAGCATCCT TTTCTTTGCT GGAAAGTAAA 
GACCATGAGG AATTGGAGCC TGCCAAAAAA AAGATGAAAG GAGATGGGGA ACTTGACACG 
AACCTCCAGT TATTAGTTTC AGGCCAGCGT GGAAATGCCC AAACCTCAAG TGAAGATGTC 
AAAGATGCCT CTGTGAAGCC AGACAAGATG GAGGAGACAC TAACCTGTAT CATCTGCCAG 
GACCTTCTGC ACGATTGTGT GAGTTTGCAG CCTTGTATGC ACACATTTTG TGCGGCTTGC 
TACTCTGGTT GGATGGAGCG TTCATCTCTG TGCCCTACCT GCCGATGTCC AGTGGAGCGG 
ATTTGCAAAA ACCACATCCT GAACAACCTA GTGGAAGCAT ACCTTATCCA GCACCCAGAT 
AAAAGTCGCA GTGAAGAAGA TGTGAGAAGT ATGGATGCAA GGAATAAAAT CACTCAAGAT 
ATGCTGCAAC CCAAAGTCAG GAGGTCTTTC TCTGATGAAG AGGGGAGTTC AGAGGACCTG 
CTAGAGCTGT CTGATGTCGA CAGTGAATCC TCAGATATCA GTCAGCCATA CATTGTCTGC 
AGACAGTGTC CTGAATACAG AAGGCAAGCG GTGCAGTCTC TTCCTTGCCC AGTCCCAGAG 
AGTGAGCTGG GAGCTACACT GGCCCTTGGT GGGGAGGCAC CTTCAACATC TGCCAGCTTG 
CCAACAGCAG CCCCGGATTA CATGTGCCCT CTTCAAGGAA GCCATGCCAT ATGCACCTGC 
TGCTTCCAGC CTATGCCTGA CCGGAGAGCT GAACGGGAGC AGGATCCCCG CGTCGCCCCT 
CAGCAGTGTG CGGTGTGCCT GCAGCCCTTC TGCCACCTGT ACTGGGGCTG CACGAGGACT 
GGCTGCTTTG GCTGCTTGGC CCCATTCTGT GAGCTCAACC TGGGGGACAA GTGCTTGGAT 
GGAGTGCTGA ACAATAACAA TTATGAATCG GACATCCTGA AGAATTACCT GGCAACCAGG 
GGTCTGACAT GGAAAAGTGT GTTGACAGAG AGTCTCCTGG CTCTGCAGCG AGGTGTATTT 
ATGCTGTCTG ATTACAGAAT CACTGGAAAT ACTGTGCTGT GTTACTGCTG TGGTCTGCGT 
AGCTTCCGAG AGCTGACCTA CCAGTATCGT CAGAACATTC CTGCTTCTGA GTTGCCAGTG 
ACTGTAACAT CCCGTCCTGA CTGCTACTGG GGCCGTAACT GTCGCACTCA GGTGAAGGCT 
CACCATGCAA TGAAATTCAA TCACATCTGT GAGCAAACAA GGTTCAAGAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006534978.1
CDS	150..2003
Translation	MRSPSNKLVSGDHCKLTVDEISGEVTLEDTSTNGTVINKLQVVKKQTYPLQSGDIIYLVYRKNEPEHNVAYLYESLSGKQSLTQDSLEANKENMFHVTKDCSGPGQGDDPQVPLLSPMAQTCLEEPQPSTSTSDLLPTASTSSTEPELTSAGQKHSSSSGPGNTSISPKGRSSLVANGELSSLSPVFQDKEASFSLLESKDHEELEPAKKKMKGDGELDTNLQLLVSGQRGNAQTSSEDVKDASVKPDKMEETLTCIICQDLLHDCVSLQPCMHTFCAACYSGWMERSSLCPTCRCPVERICKNHILNNLVEAYLIQHPDKSRSEEDVRSMDARNKITQDMLQPKVRRSFSDEEGSSEDLLELSDVDSESSDISQPYIVCRQCPEYRRQAVQSLPCPVPESELGATLALGGEAPSTSASLPTAAPDYMCPLQGSHAICTCCFQPMPDRRAEREQDPRVAPQQCAVCLQPFCHLYWGCTRTGCFGCLAPFCELNLGDKCLDGVLNNNNYESDILKNYLATRGLTWKSVLTESLLALQRGVFMLSDYRITGNTVLCYCCGLRSFRELTYQYRQNIPASELPVTVTSRPDCYWGRNCRTQVKAHHAMKFNHICEQTRFKN

Target ORF information:

RefSeq Version	XM_006534915.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus checkpoint with forkhead and ring finger domains (Chfr), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006534915.1

ORF Insert Sequence:

ATGAGATCTC CCAGCAATAA ACTGGTCTCT GGAGATCACT GTAAACTTAC AGTGGATGAA 
ATATCTGGTG AGGTGACACT GGAAGACACC AGCACCAATG GAACAGTGAT CAATAAGCTG 
CAAGTTGTTA AGAAGCAGAC TTACCCTTTA CAGAGCGGGG ATATCATCTA TTTGGTGTAC 
AGGAAGAATG AGCCAGAACA CAATGTGGCA TACCTCTATG AATCTTTAAG TGGCAAACAG 
AGCTTAACTC AAGACTCCCT TGAAGCCAAT AAGGAAAATA TGTTCCATGT GACCAAAGAT 
TGCTCAGGTC CAGGGCAGGG TGATGATCCC CAGGTTCCAC TATTGTCACC CATGGCTCAG 
ACATGCTTAG AGGAACCACA GCCATCAACA TCGACATCAG ACCTCCTCCC CACGGCCTCT 
ACCTCTTCTA CGGAGCCAGA GCTGACCTCT GCAGGGCAAA AGCATTCTTC TAGCTCTGGA 
CCTGGGAACA CAAGCATCTC CCCAAAAGGA CGCAGTTCAC TTGTTGCAAA TGGCGAACTC 
TCTAGCCTTT CTCCAGTTTT CCAAGACAAA GAAGCATCCT TTTCTTTGCT GGAAAGTAAA 
GACCATGAGG AATTGGAGCC TGCCAAAAAA AAGATGAAAG GAGATGGGGA ACTTGACACG 
AACCTCCAGT TATTAGTTTC AGGCCAGCGT GGAAATGCCC AAACCTCAAG TGAAGATGTC 
AAAGATGCCT CTGTGAAGCC AGACAAGATG GAGGAGACAC TAACCTGTAT CATCTGCCAG 
GACCTTCTGC ACGATTGTGT GAGTTTGCAG CCTTGTATGC ACACATTTTG TGCGGCTTGC 
TACTCTGGTT GGATGGAGCG TTCATCTCTG TGCCCTACCT GCCGATGTCC AGTGGAGCGG 
ATTTGCAAAA ACCACATCCT GAACAACCTA GTGGAAGCAT ACCTTATCCA GCACCCAGAT 
AAAAGTCGCA GTGAAGAAGA TGTGAGAAGT ATGGATGCAA GGAATAAAAT CACTCAAGAT 
ATGCTGCAAC CCAAAGTCAG GAGGTCTTTC TCTGATGAAG AGGGGAGTTC AGAGGACCTG 
CTAGAGCTGT CTGATGTCGA CAGTGAATCC TCAGATATCA GTCAGCCATA CATTGTCTGC 
AGACAGTGTC CTGAATACAG AAGGCAAGCG GTGCAGTCTC TTCCTTGCCC AGTCCCAGAG 
AGTGAGCTGG GAGCTACACT GGCCCTTGGT GGGGAGGCAC CTTCAACATC TGCCAGCTTG 
CCAACAGCAG CCCCGGATTA CATGTGCCCT CTTCAAGGAA GCCATGCCAT ATGCACCTGC 
TGCTTCCAGC CTATGCCTGA CCGGAGAGCT GAACGGGAGC AGGATCCCCG CGTCGCCCCT 
CAGCAGTGTG CGGTGTGCCT GCAGCCCTTC TGCCACCTGT ACTGGGGCTG CACGAGGACT 
GGCTGCTTTG GCTGCTTGGC CCCATTCTGT GAGCTCAACC TGGGGGACAA GTGCTTGGAT 
GGAGTGCTGA ACAATAACAA TTATGAATCG GACATCCTGA AGAATTACCT GGCAACCAGG 
GGTCTGACAT GGAAAAGTGT GTTGACAGAG AGTCTCCTGG CTCTGCAGCG AGGTGTATTT 
ATGCTGTCTG ATTACAGAAT CACTGGAAAT ACTGTGCTGT GTTACTGCTG TGGTCTGCGT 
AGCTTCCGAG AGCTGACCTA CCAGTATCGT CAGAACATTC CTGCTTCTGA GTTGCCAGTG 
ACTGTAACAT CCCGTCCTGA CTGCTACTGG GGCCGTAACT GTCGCACTCA GGTGAAGGCT 
CACCATGCAA TGAAATTCAA TCACATCTGT GAGCAAACAA GGTTCAAGAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chfr ( XM_030254420.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030110280.1 Mus musculus checkpoint with forkhead and ring finger domains (Chfr), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OMu88879
Clone ID Related Accession (Same CDS sequence)	XM_030254420.1
Accession Version	XM_030254420.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1107bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase CHFR isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGA CACTAACCTG TATCATCTGC CAGGACCTTC TGCACGATTG TGTGAGTTTG 
CAGCCTTGTA TGCACACATT TTGTGCGGCT TGCTACTCTG GTTGGATGGA GCGTTCATCT 
CTGTGCCCTA CCTGCCGATG TCCAGTGGAG CGGATTTGCA AAAACCACAT CCTGAACAAC 
CTAGTGGAAG CATACCTTAT CCAGCACCCA GATAAAAGTC GCAGTGAAGA AGATGTGAGA 
AGTATGGATG CAAGGAATAA AATCACTCAA GATATGCTGC AACCCAAAGT CAGGAGGTCT 
TTCTCTGATG AAGAGGGGAG TTCAGAGGAC CTGCTAGAGC TGTCTGATGT CGACAGTGAA 
TCCTCAGATA TCAGTCAGCC ATACATTGTC TGCAGACAGT GTCCTGAATA CAGAAGGCAA 
GCGGTGCAGT CTCTTCCTTG CCCAGTCCCA GAGAGTGAGC TGGGAGCTAC ACTGGCCCTT 
GGTGGGGAGG CACCTTCAAC ATCTGCCAGC TTGCCAACAG CAGCCCCGGA TTACATGTGC 
CCTCTTCAAG GAAGCCATGC CATATGCACC TGCTGCTTCC AGCCTATGCC TGACCGGAGA 
GCTGAACGGG AGCAGGATCC CCGCGTCGCC CCTCAGCAGT GTGCGGTGTG CCTGCAGCCC 
TTCTGCCACC TGTACTGGGG CTGCACGAGG ACTGGCTGCT TTGGCTGCTT GGCCCCATTC 
TGTGAGCTCA ACCTGGGGGA CAAGTGCTTG GATGGAGTGC TGAACAATAA CAATTATGAA 
TCGGACATCC TGAAGAATTA CCTGGCAACC AGGGGTCTGA CATGGAAAAG TGTGTTGACA 
GAGAGTCTCC TGGCTCTGCA GCGAGGTGTA TTTATGCTGT CTGATTACAG AATCACTGGA 
AATACTGTGC TGTGTTACTG CTGTGGTCTG CGTAGCTTCC GAGAGCTGAC CTACCAGTAT 
CGTCAGAACA TTCCTGCTTC TGAGTTGCCA GTGACTGTAA CATCCCGTCC TGACTGCTAC 
TGGGGCCGTA ACTGTCGCAC TCAGGTGAAG GCTCACCATG CAATGAAATT CAATCACATC 
TGTGAGCAAA CAAGGTTCAA GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030110280.1
CDS	1176..2282
Translation	MEETLTCIICQDLLHDCVSLQPCMHTFCAACYSGWMERSSLCPTCRCPVERICKNHILNNLVEAYLIQHPDKSRSEEDVRSMDARNKITQDMLQPKVRRSFSDEEGSSEDLLELSDVDSESSDISQPYIVCRQCPEYRRQAVQSLPCPVPESELGATLALGGEAPSTSASLPTAAPDYMCPLQGSHAICTCCFQPMPDRRAEREQDPRVAPQQCAVCLQPFCHLYWGCTRTGCFGCLAPFCELNLGDKCLDGVLNNNNYESDILKNYLATRGLTWKSVLTESLLALQRGVFMLSDYRITGNTVLCYCCGLRSFRELTYQYRQNIPASELPVTVTSRPDCYWGRNCRTQVKAHHAMKFNHICEQTRFKN

Target ORF information:

RefSeq Version	XM_030254420.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus checkpoint with forkhead and ring finger domains (Chfr), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030254420.1

ORF Insert Sequence:

ATGGAGGAGA CACTAACCTG TATCATCTGC CAGGACCTTC TGCACGATTG TGTGAGTTTG 
CAGCCTTGTA TGCACACATT TTGTGCGGCT TGCTACTCTG GTTGGATGGA GCGTTCATCT 
CTGTGCCCTA CCTGCCGATG TCCAGTGGAG CGGATTTGCA AAAACCACAT CCTGAACAAC 
CTAGTGGAAG CATACCTTAT CCAGCACCCA GATAAAAGTC GCAGTGAAGA AGATGTGAGA 
AGTATGGATG CAAGGAATAA AATCACTCAA GATATGCTGC AACCCAAAGT CAGGAGGTCT 
TTCTCTGATG AAGAGGGGAG TTCAGAGGAC CTGCTAGAGC TGTCTGATGT CGACAGTGAA 
TCCTCAGATA TCAGTCAGCC ATACATTGTC TGCAGACAGT GTCCTGAATA CAGAAGGCAA 
GCGGTGCAGT CTCTTCCTTG CCCAGTCCCA GAGAGTGAGC TGGGAGCTAC ACTGGCCCTT 
GGTGGGGAGG CACCTTCAAC ATCTGCCAGC TTGCCAACAG CAGCCCCGGA TTACATGTGC 
CCTCTTCAAG GAAGCCATGC CATATGCACC TGCTGCTTCC AGCCTATGCC TGACCGGAGA 
GCTGAACGGG AGCAGGATCC CCGCGTCGCC CCTCAGCAGT GTGCGGTGTG CCTGCAGCCC 
TTCTGCCACC TGTACTGGGG CTGCACGAGG ACTGGCTGCT TTGGCTGCTT GGCCCCATTC 
TGTGAGCTCA ACCTGGGGGA CAAGTGCTTG GATGGAGTGC TGAACAATAA CAATTATGAA 
TCGGACATCC TGAAGAATTA CCTGGCAACC AGGGGTCTGA CATGGAAAAG TGTGTTGACA 
GAGAGTCTCC TGGCTCTGCA GCGAGGTGTA TTTATGCTGT CTGATTACAG AATCACTGGA 
AATACTGTGC TGTGTTACTG CTGTGGTCTG CGTAGCTTCC GAGAGCTGAC CTACCAGTAT 
CGTCAGAACA TTCCTGCTTC TGAGTTGCCA GTGACTGTAA CATCCCGTCC TGACTGCTAC 
TGGGGCCGTA ACTGTCGCAC TCAGGTGAAG GCTCACCATG CAATGAAATT CAATCACATC 
TGTGAGCAAA CAAGGTTCAA GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chfr ( XM_030254421.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030110281.1 Mus musculus checkpoint with forkhead and ring finger domains (Chfr), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OMu88880
Clone ID Related Accession (Same CDS sequence)	XM_030254421.1
Accession Version	XM_030254421.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 861bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase CHFR isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGCAA GGAATAAAAT CACTCAAGAT ATGCTGCAAC CCAAAGTCAG GAGGTCTTTC 
TCTGATGAAG AGGGGAGTTC AGAGGACCTG CTAGAGCTGT CTGATGTCGA CAGTGAATCC 
TCAGATATCA GTCAGCCATA CATTGTCTGC AGACAGTGTC CTGAATACAG AAGGCAAGCG 
GTGCAGTCTC TTCCTTGCCC AGTCCCAGAG AGTGAGCTGG GAGCTACACT GGCCCTTGGT 
GGGGAGGCAC CTTCAACATC TGCCAGCTTG CCAACAGCCC CGGATTACAT GTGCCCTCTT 
CAAGGAAGCC ATGCCATATG CACCTGCTGC TTCCAGCCTA TGCCTGACCG GAGAGCTGAA 
CGGGAGCAGG ATCCCCGCGT CGCCCCTCAG CAGTGTGCGG TGTGCCTGCA GCCCTTCTGC 
CACCTGTACT GGGGCTGCAC GAGGACTGGC TGCTTTGGCT GCTTGGCCCC ATTCTGTGAG 
CTCAACCTGG GGGACAAGTG CTTGGATGGA GTGCTGAACA ATAACAATTA TGAATCGGAC 
ATCCTGAAGA ATTACCTGGC AACCAGGGGT CTGACATGGA AAAGTGTGTT GACAGAGAGT 
CTCCTGGCTC TGCAGCGAGG TGTATTTATG CTGTCTGATT ACAGAATCAC TGGAAATACT 
GTGCTGTGTT ACTGCTGTGG TCTGCGTAGC TTCCGAGAGC TGACCTACCA GTATCGTCAG 
AACATTCCTG CTTCTGAGTT GCCAGTGACT GTAACATCCC GTCCTGACTG CTACTGGGGC 
CGTAACTGTC GCACTCAGGT GAAGGCTCAC CATGCAATGA AATTCAATCA CATCTGTGAG 
CAAACAAGGT TCAAGAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030110281.1
CDS	1554..2414
Translation	MDARNKITQDMLQPKVRRSFSDEEGSSEDLLELSDVDSESSDISQPYIVCRQCPEYRRQAVQSLPCPVPESELGATLALGGEAPSTSASLPTAPDYMCPLQGSHAICTCCFQPMPDRRAEREQDPRVAPQQCAVCLQPFCHLYWGCTRTGCFGCLAPFCELNLGDKCLDGVLNNNNYESDILKNYLATRGLTWKSVLTESLLALQRGVFMLSDYRITGNTVLCYCCGLRSFRELTYQYRQNIPASELPVTVTSRPDCYWGRNCRTQVKAHHAMKFNHICEQTRFKN

Target ORF information:

RefSeq Version	XM_030254421.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus checkpoint with forkhead and ring finger domains (Chfr), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030254421.1

ORF Insert Sequence:

ATGGATGCAA GGAATAAAAT CACTCAAGAT ATGCTGCAAC CCAAAGTCAG GAGGTCTTTC 
TCTGATGAAG AGGGGAGTTC AGAGGACCTG CTAGAGCTGT CTGATGTCGA CAGTGAATCC 
TCAGATATCA GTCAGCCATA CATTGTCTGC AGACAGTGTC CTGAATACAG AAGGCAAGCG 
GTGCAGTCTC TTCCTTGCCC AGTCCCAGAG AGTGAGCTGG GAGCTACACT GGCCCTTGGT 
GGGGAGGCAC CTTCAACATC TGCCAGCTTG CCAACAGCCC CGGATTACAT GTGCCCTCTT 
CAAGGAAGCC ATGCCATATG CACCTGCTGC TTCCAGCCTA TGCCTGACCG GAGAGCTGAA 
CGGGAGCAGG ATCCCCGCGT CGCCCCTCAG CAGTGTGCGG TGTGCCTGCA GCCCTTCTGC 
CACCTGTACT GGGGCTGCAC GAGGACTGGC TGCTTTGGCT GCTTGGCCCC ATTCTGTGAG 
CTCAACCTGG GGGACAAGTG CTTGGATGGA GTGCTGAACA ATAACAATTA TGAATCGGAC 
ATCCTGAAGA ATTACCTGGC AACCAGGGGT CTGACATGGA AAAGTGTGTT GACAGAGAGT 
CTCCTGGCTC TGCAGCGAGG TGTATTTATG CTGTCTGATT ACAGAATCAC TGGAAATACT 
GTGCTGTGTT ACTGCTGTGG TCTGCGTAGC TTCCGAGAGC TGACCTACCA GTATCGTCAG 
AACATTCCTG CTTCTGAGTT GCCAGTGACT GTAACATCCC GTCCTGACTG CTACTGGGGC 
CGTAACTGTC GCACTCAGGT GAAGGCTCAC CATGCAATGA AATTCAATCA CATCTGTGAG 
CAAACAAGGT TCAAGAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chfr ( XM_030254419.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030110279.1 Mus musculus checkpoint with forkhead and ring finger domains (Chfr), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OMu16462
Clone ID Related Accession (Same CDS sequence)	NM_001289580.1 , XM_030254419.1 , XM_030254418.1
Accession Version	XM_030254419.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1572bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase CHFR isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCATG TGACCAAAGA TTGCTCAGGT CCAGGGCAGG GTGATGATCC CCAGGTTCCA 
CTATTGTCAC CCATGGCTCA GACATGCTTA GAGGAACCAC AGCCATCAAC ATCGACATCA 
GACCTCCTCC CCACGGCCTC TACCTCTTCT ACGGAGCCAG AGCTGACCTC TGCAGGGCAA 
AAGCATTCTT CTAGCTCTGG ACCTGGGAAC ACAAGCATCT CCCCAAAAGG ACGCAGTTCA 
CTTGTTGCAA ATGGCGAACT CTCTAGCCTT TCTCCAGTTT TCCAAGACAA AGAAGCATCC 
TTTTCTTTGC TGGAAAGTAA AGACCATGAG GAATTGGAGC CTGCCAAAAA AAAGATGAAA 
GGAGATGGGG AACTTGACAC GAACCTCCAG TTATTAGTTT CAGGCCAGCG TGGAAATGCC 
CAAACCTCAA GTGAAGATGT CAAAGATGCC TCTGTGAAGC CAGACAAGAT GGAGGAGACA 
CTAACCTGTA TCATCTGCCA GGACCTTCTG CACGATTGTG TGAGTTTGCA GCCTTGTATG 
CACACATTTT GTGCGGCTTG CTACTCTGGT TGGATGGAGC GTTCATCTCT GTGCCCTACC 
TGCCGATGTC CAGTGGAGCG GATTTGCAAA AACCACATCC TGAACAACCT AGTGGAAGCA 
TACCTTATCC AGCACCCAGA TAAAAGTCGC AGTGAAGAAG ATGTGAGAAG TATGGATGCA 
AGGAATAAAA TCACTCAAGA TATGCTGCAA CCCAAAGTCA GGAGGTCTTT CTCTGATGAA 
GAGGGGAGTT CAGAGGACCT GCTAGAGCTG TCTGATGTCG ACAGTGAATC CTCAGATATC 
AGTCAGCCAT ACATTGTCTG CAGACAGTGT CCTGAATACA GAAGGCAAGC GGTGCAGTCT 
CTTCCTTGCC CAGTCCCAGA GAGTGAGCTG GGAGCTACAC TGGCCCTTGG TGGGGAGGCA 
CCTTCAACAT CTGCCAGCTT GCCAACAGCC CCGGATTACA TGTGCCCTCT TCAAGGAAGC 
CATGCCATAT GCACCTGCTG CTTCCAGCCT ATGCCTGACC GGAGAGCTGA ACGGGAGCAG 
GATCCCCGCG TCGCCCCTCA GCAGTGTGCG GTGTGCCTGC AGCCCTTCTG CCACCTGTAC 
TGGGGCTGCA CGAGGACTGG CTGCTTTGGC TGCTTGGCCC CATTCTGTGA GCTCAACCTG 
GGGGACAAGT GCTTGGATGG AGTGCTGAAC AATAACAATT ATGAATCGGA CATCCTGAAG 
AATTACCTGG CAACCAGGGG TCTGACATGG AAAAGTGTGT TGACAGAGAG TCTCCTGGCT 
CTGCAGCGAG GTGTATTTAT GCTGTCTGAT TACAGAATCA CTGGAAATAC TGTGCTGTGT 
TACTGCTGTG GTCTGCGTAG CTTCCGAGAG CTGACCTACC AGTATCGTCA GAACATTCCT 
GCTTCTGAGT TGCCAGTGAC TGTAACATCC CGTCCTGACT GCTACTGGGG CCGTAACTGT 
CGCACTCAGG TGAAGGCTCA CCATGCAATG AAATTCAATC ACATCTGTGA GCAAACAAGG 
TTCAAGAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030110279.1
CDS	412..1983
Translation	MFHVTKDCSGPGQGDDPQVPLLSPMAQTCLEEPQPSTSTSDLLPTASTSSTEPELTSAGQKHSSSSGPGNTSISPKGRSSLVANGELSSLSPVFQDKEASFSLLESKDHEELEPAKKKMKGDGELDTNLQLLVSGQRGNAQTSSEDVKDASVKPDKMEETLTCIICQDLLHDCVSLQPCMHTFCAACYSGWMERSSLCPTCRCPVERICKNHILNNLVEAYLIQHPDKSRSEEDVRSMDARNKITQDMLQPKVRRSFSDEEGSSEDLLELSDVDSESSDISQPYIVCRQCPEYRRQAVQSLPCPVPESELGATLALGGEAPSTSASLPTAPDYMCPLQGSHAICTCCFQPMPDRRAEREQDPRVAPQQCAVCLQPFCHLYWGCTRTGCFGCLAPFCELNLGDKCLDGVLNNNNYESDILKNYLATRGLTWKSVLTESLLALQRGVFMLSDYRITGNTVLCYCCGLRSFRELTYQYRQNIPASELPVTVTSRPDCYWGRNCRTQVKAHHAMKFNHICEQTRFKN

Target ORF information:

RefSeq Version	XM_030254419.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus checkpoint with forkhead and ring finger domains (Chfr), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030254419.1

ORF Insert Sequence:

ATGTTCCATG TGACCAAAGA TTGCTCAGGT CCAGGGCAGG GTGATGATCC CCAGGTTCCA 
CTATTGTCAC CCATGGCTCA GACATGCTTA GAGGAACCAC AGCCATCAAC ATCGACATCA 
GACCTCCTCC CCACGGCCTC TACCTCTTCT ACGGAGCCAG AGCTGACCTC TGCAGGGCAA 
AAGCATTCTT CTAGCTCTGG ACCTGGGAAC ACAAGCATCT CCCCAAAAGG ACGCAGTTCA 
CTTGTTGCAA ATGGCGAACT CTCTAGCCTT TCTCCAGTTT TCCAAGACAA AGAAGCATCC 
TTTTCTTTGC TGGAAAGTAA AGACCATGAG GAATTGGAGC CTGCCAAAAA AAAGATGAAA 
GGAGATGGGG AACTTGACAC GAACCTCCAG TTATTAGTTT CAGGCCAGCG TGGAAATGCC 
CAAACCTCAA GTGAAGATGT CAAAGATGCC TCTGTGAAGC CAGACAAGAT GGAGGAGACA 
CTAACCTGTA TCATCTGCCA GGACCTTCTG CACGATTGTG TGAGTTTGCA GCCTTGTATG 
CACACATTTT GTGCGGCTTG CTACTCTGGT TGGATGGAGC GTTCATCTCT GTGCCCTACC 
TGCCGATGTC CAGTGGAGCG GATTTGCAAA AACCACATCC TGAACAACCT AGTGGAAGCA 
TACCTTATCC AGCACCCAGA TAAAAGTCGC AGTGAAGAAG ATGTGAGAAG TATGGATGCA 
AGGAATAAAA TCACTCAAGA TATGCTGCAA CCCAAAGTCA GGAGGTCTTT CTCTGATGAA 
GAGGGGAGTT CAGAGGACCT GCTAGAGCTG TCTGATGTCG ACAGTGAATC CTCAGATATC 
AGTCAGCCAT ACATTGTCTG CAGACAGTGT CCTGAATACA GAAGGCAAGC GGTGCAGTCT 
CTTCCTTGCC CAGTCCCAGA GAGTGAGCTG GGAGCTACAC TGGCCCTTGG TGGGGAGGCA 
CCTTCAACAT CTGCCAGCTT GCCAACAGCC CCGGATTACA TGTGCCCTCT TCAAGGAAGC 
CATGCCATAT GCACCTGCTG CTTCCAGCCT ATGCCTGACC GGAGAGCTGA ACGGGAGCAG 
GATCCCCGCG TCGCCCCTCA GCAGTGTGCG GTGTGCCTGC AGCCCTTCTG CCACCTGTAC 
TGGGGCTGCA CGAGGACTGG CTGCTTTGGC TGCTTGGCCC CATTCTGTGA GCTCAACCTG 
GGGGACAAGT GCTTGGATGG AGTGCTGAAC AATAACAATT ATGAATCGGA CATCCTGAAG 
AATTACCTGG CAACCAGGGG TCTGACATGG AAAAGTGTGT TGACAGAGAG TCTCCTGGCT 
CTGCAGCGAG GTGTATTTAT GCTGTCTGAT TACAGAATCA CTGGAAATAC TGTGCTGTGT 
TACTGCTGTG GTCTGCGTAG CTTCCGAGAG CTGACCTACC AGTATCGTCA GAACATTCCT 
GCTTCTGAGT TGCCAGTGAC TGTAACATCC CGTCCTGACT GCTACTGGGG CCGTAACTGT 
CGCACTCAGG TGAAGGCTCA CCATGCAATG AAATTCAATC ACATCTGTGA GCAAACAAGG 
TTCAAGAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chfr ( XM_030254418.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030110278.1 Mus musculus checkpoint with forkhead and ring finger domains (Chfr), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu16462
Clone ID Related Accession (Same CDS sequence)	NM_001289580.1 , XM_030254419.1 , XM_030254418.1
Accession Version	XM_030254418.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1572bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase CHFR isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCATG TGACCAAAGA TTGCTCAGGT CCAGGGCAGG GTGATGATCC CCAGGTTCCA 
CTATTGTCAC CCATGGCTCA GACATGCTTA GAGGAACCAC AGCCATCAAC ATCGACATCA 
GACCTCCTCC CCACGGCCTC TACCTCTTCT ACGGAGCCAG AGCTGACCTC TGCAGGGCAA 
AAGCATTCTT CTAGCTCTGG ACCTGGGAAC ACAAGCATCT CCCCAAAAGG ACGCAGTTCA 
CTTGTTGCAA ATGGCGAACT CTCTAGCCTT TCTCCAGTTT TCCAAGACAA AGAAGCATCC 
TTTTCTTTGC TGGAAAGTAA AGACCATGAG GAATTGGAGC CTGCCAAAAA AAAGATGAAA 
GGAGATGGGG AACTTGACAC GAACCTCCAG TTATTAGTTT CAGGCCAGCG TGGAAATGCC 
CAAACCTCAA GTGAAGATGT CAAAGATGCC TCTGTGAAGC CAGACAAGAT GGAGGAGACA 
CTAACCTGTA TCATCTGCCA GGACCTTCTG CACGATTGTG TGAGTTTGCA GCCTTGTATG 
CACACATTTT GTGCGGCTTG CTACTCTGGT TGGATGGAGC GTTCATCTCT GTGCCCTACC 
TGCCGATGTC CAGTGGAGCG GATTTGCAAA AACCACATCC TGAACAACCT AGTGGAAGCA 
TACCTTATCC AGCACCCAGA TAAAAGTCGC AGTGAAGAAG ATGTGAGAAG TATGGATGCA 
AGGAATAAAA TCACTCAAGA TATGCTGCAA CCCAAAGTCA GGAGGTCTTT CTCTGATGAA 
GAGGGGAGTT CAGAGGACCT GCTAGAGCTG TCTGATGTCG ACAGTGAATC CTCAGATATC 
AGTCAGCCAT ACATTGTCTG CAGACAGTGT CCTGAATACA GAAGGCAAGC GGTGCAGTCT 
CTTCCTTGCC CAGTCCCAGA GAGTGAGCTG GGAGCTACAC TGGCCCTTGG TGGGGAGGCA 
CCTTCAACAT CTGCCAGCTT GCCAACAGCC CCGGATTACA TGTGCCCTCT TCAAGGAAGC 
CATGCCATAT GCACCTGCTG CTTCCAGCCT ATGCCTGACC GGAGAGCTGA ACGGGAGCAG 
GATCCCCGCG TCGCCCCTCA GCAGTGTGCG GTGTGCCTGC AGCCCTTCTG CCACCTGTAC 
TGGGGCTGCA CGAGGACTGG CTGCTTTGGC TGCTTGGCCC CATTCTGTGA GCTCAACCTG 
GGGGACAAGT GCTTGGATGG AGTGCTGAAC AATAACAATT ATGAATCGGA CATCCTGAAG 
AATTACCTGG CAACCAGGGG TCTGACATGG AAAAGTGTGT TGACAGAGAG TCTCCTGGCT 
CTGCAGCGAG GTGTATTTAT GCTGTCTGAT TACAGAATCA CTGGAAATAC TGTGCTGTGT 
TACTGCTGTG GTCTGCGTAG CTTCCGAGAG CTGACCTACC AGTATCGTCA GAACATTCCT 
GCTTCTGAGT TGCCAGTGAC TGTAACATCC CGTCCTGACT GCTACTGGGG CCGTAACTGT 
CGCACTCAGG TGAAGGCTCA CCATGCAATG AAATTCAATC ACATCTGTGA GCAAACAAGG 
TTCAAGAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030110278.1
CDS	580..2151
Translation	MFHVTKDCSGPGQGDDPQVPLLSPMAQTCLEEPQPSTSTSDLLPTASTSSTEPELTSAGQKHSSSSGPGNTSISPKGRSSLVANGELSSLSPVFQDKEASFSLLESKDHEELEPAKKKMKGDGELDTNLQLLVSGQRGNAQTSSEDVKDASVKPDKMEETLTCIICQDLLHDCVSLQPCMHTFCAACYSGWMERSSLCPTCRCPVERICKNHILNNLVEAYLIQHPDKSRSEEDVRSMDARNKITQDMLQPKVRRSFSDEEGSSEDLLELSDVDSESSDISQPYIVCRQCPEYRRQAVQSLPCPVPESELGATLALGGEAPSTSASLPTAPDYMCPLQGSHAICTCCFQPMPDRRAEREQDPRVAPQQCAVCLQPFCHLYWGCTRTGCFGCLAPFCELNLGDKCLDGVLNNNNYESDILKNYLATRGLTWKSVLTESLLALQRGVFMLSDYRITGNTVLCYCCGLRSFRELTYQYRQNIPASELPVTVTSRPDCYWGRNCRTQVKAHHAMKFNHICEQTRFKN

Target ORF information:

RefSeq Version	XM_030254418.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus checkpoint with forkhead and ring finger domains (Chfr), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030254418.1

ORF Insert Sequence:

ATGTTCCATG TGACCAAAGA TTGCTCAGGT CCAGGGCAGG GTGATGATCC CCAGGTTCCA 
CTATTGTCAC CCATGGCTCA GACATGCTTA GAGGAACCAC AGCCATCAAC ATCGACATCA 
GACCTCCTCC CCACGGCCTC TACCTCTTCT ACGGAGCCAG AGCTGACCTC TGCAGGGCAA 
AAGCATTCTT CTAGCTCTGG ACCTGGGAAC ACAAGCATCT CCCCAAAAGG ACGCAGTTCA 
CTTGTTGCAA ATGGCGAACT CTCTAGCCTT TCTCCAGTTT TCCAAGACAA AGAAGCATCC 
TTTTCTTTGC TGGAAAGTAA AGACCATGAG GAATTGGAGC CTGCCAAAAA AAAGATGAAA 
GGAGATGGGG AACTTGACAC GAACCTCCAG TTATTAGTTT CAGGCCAGCG TGGAAATGCC 
CAAACCTCAA GTGAAGATGT CAAAGATGCC TCTGTGAAGC CAGACAAGAT GGAGGAGACA 
CTAACCTGTA TCATCTGCCA GGACCTTCTG CACGATTGTG TGAGTTTGCA GCCTTGTATG 
CACACATTTT GTGCGGCTTG CTACTCTGGT TGGATGGAGC GTTCATCTCT GTGCCCTACC 
TGCCGATGTC CAGTGGAGCG GATTTGCAAA AACCACATCC TGAACAACCT AGTGGAAGCA 
TACCTTATCC AGCACCCAGA TAAAAGTCGC AGTGAAGAAG ATGTGAGAAG TATGGATGCA 
AGGAATAAAA TCACTCAAGA TATGCTGCAA CCCAAAGTCA GGAGGTCTTT CTCTGATGAA 
GAGGGGAGTT CAGAGGACCT GCTAGAGCTG TCTGATGTCG ACAGTGAATC CTCAGATATC 
AGTCAGCCAT ACATTGTCTG CAGACAGTGT CCTGAATACA GAAGGCAAGC GGTGCAGTCT 
CTTCCTTGCC CAGTCCCAGA GAGTGAGCTG GGAGCTACAC TGGCCCTTGG TGGGGAGGCA 
CCTTCAACAT CTGCCAGCTT GCCAACAGCC CCGGATTACA TGTGCCCTCT TCAAGGAAGC 
CATGCCATAT GCACCTGCTG CTTCCAGCCT ATGCCTGACC GGAGAGCTGA ACGGGAGCAG 
GATCCCCGCG TCGCCCCTCA GCAGTGTGCG GTGTGCCTGC AGCCCTTCTG CCACCTGTAC 
TGGGGCTGCA CGAGGACTGG CTGCTTTGGC TGCTTGGCCC CATTCTGTGA GCTCAACCTG 
GGGGACAAGT GCTTGGATGG AGTGCTGAAC AATAACAATT ATGAATCGGA CATCCTGAAG 
AATTACCTGG CAACCAGGGG TCTGACATGG AAAAGTGTGT TGACAGAGAG TCTCCTGGCT 
CTGCAGCGAG GTGTATTTAT GCTGTCTGAT TACAGAATCA CTGGAAATAC TGTGCTGTGT 
TACTGCTGTG GTCTGCGTAG CTTCCGAGAG CTGACCTACC AGTATCGTCA GAACATTCCT 
GCTTCTGAGT TGCCAGTGAC TGTAACATCC CGTCCTGACT GCTACTGGGG CCGTAACTGT 
CGCACTCAGG TGAAGGCTCA CCATGCAATG AAATTCAATC ACATCTGTGA GCAAACAAGG 
TTCAAGAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	Chfr
Entrez Gene ID	231600
Full Name	checkpoint with forkhead and ring finger domains
Synonyms	5730484M20Rik,C230082M18,RNF116
General protein information	Preferred Names checkpoint with forkhead and ring finger domains Names E3 ubiquitin-protein ligase CHFR RING-type E3 ubiquitin transferase CHFR
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5\|5 F

Rattus norvegicus (Norway rat)	Chfr	NP_001009258.1
Xenopus tropicalis (tropical clawed frog)	chfr	NP_989378.1
	CHFR	XP_001083796.2
Oryza sativa (rice)	Os11g0175500	NP_001065879.2
Homo sapiens (human)	CHFR	NP_001154816.1
Pan troglodytes (chimpanzee)	CHFR	XP_003314094.2
Bos taurus (cattle)	CHFR	NP_001192403.1
Danio rerio (zebrafish)	chfr	NP_001093485.1
Canis lupus familiaris (dog)	CHFR	XP_534631.3
Arabidopsis thaliana (thale cress)	AT1G47570	NP_001077687.1
Mus musculus (house mouse)	Chfr	NP_766305.2
Gallus gallus (chicken)	CHFR	XP_004945549.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

Chfr cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

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