chfr cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following chfr gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the chfr cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa00419 NM_204047.1
Latest version!
Xenopus tropicalis checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase (chfr), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OXa00419 XM_012958542.3
Latest version!
Xenopus tropicalis checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase (chfr), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OXa00419 XM_012958547.3
Latest version!
Xenopus tropicalis checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase (chfr), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OXa41728 XM_018091888.1
Latest version!
Xenopus tropicalis checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase (chfr), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $342.30
$489.00
View Old Accession Versions >>
OXa00419 XM_012958542.2 Xenopus tropicalis checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase (chfr), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OXa00419 XM_012958547.2 Xenopus tropicalis checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase (chfr), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa00419
    Clone ID Related Accession (Same CDS sequence) XM_012958547.2 , XM_012958542.3 , NM_204047.1 , XM_012958542.2 , XM_012958547.3
    Accession Version NM_204047.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1881bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-21
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase CHFR
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC064721.1. ##Evidence-Data-START## Transcript exon combination :: BC064721.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00028290, SAMD00028291 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGATGGCT TAGGGGAGAA GAAGCCCTGG GGGAAGCTAA GTAGATTGTT GGGAGCTGAG 
    ACGGAATCCT CATCTGAACT GTTTCTCTAC AAAAAGGAAT GGACAATAGG CAGAAAGAAA
    GCTTGTGACC TGTCTTTCCC TGGAAACAAG TTAGTGTCTG GAGAACACTG CAAAATTACA
    GTGAATGAAG AATCTGGTAG TGTTTCCTTA GAGGACACCA GCACAAATGG CACAGTAATC
    AACAAGCTGA AAGTGGTAAA GAAGCAGACA TACCCCTTGA AAAATGGTGA TGTGATCTAT
    GTTGTTTATA GGAAAAATGA GCCAGAACAA AATGTTGCTT ACCTATACAA ATCCTTAAAC
    CAGGCAGAAG ATAGTATGCA GAATCCAGCA GATACCTCAG GTTCAGAGGA AGCTGACACT
    CAGACTTTGT CATCTCAAGA TGATCAGCTG TCATATGAGG AACCTCAGCC GTCCACTTCC
    ACATCCAGTC TCTTCAGCAC ACCTACTACC TCTGCAAGTG AGCCAGCTTG CACTCTCTTG
    TATAAGTCCC AGGCATCAGT TCCTGGGGTA CAGCTTGAAA GTGGTGAGAA GTCTGGGGAA
    AGTCTTGAAG GACACAGCTC CACATCGGAT GCAACAGCAC ATGAGAAGGG ATCTTTAGGT
    CCTCCAAAAA AGAGAATGAG AACAGAGGAC CTCTGGACGG GCAACAAAAA TCTTGTTTCT
    GCAAGTTGTT CCAAAGGAGC AAGCGATGAA AGCAAGACAC CAAGTATGAA GCCAGATAAG
    ATGGAGGAAA CGCTCACCTG TATAATCTGT CAGGAATTGC TGCACGACTG TGTCAGTTTG
    CAGCCATGTA TGCACACTTT TTGTGCTGCT TGTTATTCTG GTTGGATGGA ACGCTCTTCC
    CTCTGTCCTA CCTGCCGCTG CCCTGTTGAA CGTATCTGTA AAAACCATAT ACTGAACAAT
    TTGGTGGAGG CCTATTTGAT CCAGCATCCA GAGAAATGCC GCAGTGAGGA AGACAGGTGT
    AGTATGGATG CACGGAATAA GATTACACAA GATATGTTGC AGCCAAAGGT ACGTCGGTCG
    TTTTCTGATG AAGAAGGGAG CTCTGAGGAT CTGCTGGAGC TTTCTGATGT GGATAGTGAA
    TCTTCTGATA TAAGCCAGCC TTATACTGTA TGCAGACAGT GCCCTGGATA TGTCCGCCAC
    AATATTCAAC CCCCACCATA TCCTCCACCT TCTGACACAG AGGCTTCACG TACTCAAGGA
    GATGCCCCTT CTACCTCTAC TAACTTTCCT ACAGCTACCC AGGAATATGT GTGCCCTTCT
    CATGGAAGCC ACGTGATCTG CACCTGCTGC TTCCAGCCGA TGCCAGACCG AAGGGCAGAA
    CGGGAGCACA ACTCTCATGT GGCACCTCAG CAATGCACTA TATGTTTGGA GCCCTTCTGT
    CACATGTACT GGGGATGCAA CCGGATGGGT TGTTTTGGGT GTTTAGCTCC ATTCTGTGAG
    TTGAATCTGG GAGACAAATG TTTGGATGGA GTTTTAAATA ACAATAACTA TGAATCTGAC
    ATCCTTAAGA ATTACCTGGC ATCCAGGGGA CTCACATGGA AAGATATGCT GAATGAAAGC
    TTGTCAGCTG CTCAGAGAGG GGTGTTTATG CTTCCTGATT ACAGGATAAA TGGAACCACT
    GTACTCTGCT ACTTCTGCGG GCTGCGGAAC TTTAGAATTC TAACATACCA GTACAGACAA
    AACATCCCAG CTTCAGAGCT ACCAGTGACT GTTACTTCTC GGCCAAATTG CTATTGGGGG
    CGGAATTGCA GAACTCAGGT TAAAGCTCAC CATGCTATGA AATTCAATCA CATATGTGAA
    CAAACCCGCT TCAAGAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_989378.1
    CDS31..1911
    Translation

    Target ORF information:

    RefSeq Version NM_204047.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase (chfr), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_204047.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGATGGCT TAGGGGAGAA GAAGCCCTGG GGGAAGCTAA GTAGATTGTT GGGAGCTGAG 
    ACGGAATCCT CATCTGAACT GTTTCTCTAC AAAAAGGAAT GGACAATAGG CAGAAAGAAA
    GCTTGTGACC TGTCTTTCCC TGGAAACAAG TTAGTGTCTG GAGAACACTG CAAAATTACA
    GTGAATGAAG AATCTGGTAG TGTTTCCTTA GAGGACACCA GCACAAATGG CACAGTAATC
    AACAAGCTGA AAGTGGTAAA GAAGCAGACA TACCCCTTGA AAAATGGTGA TGTGATCTAT
    GTTGTTTATA GGAAAAATGA GCCAGAACAA AATGTTGCTT ACCTATACAA ATCCTTAAAC
    CAGGCAGAAG ATAGTATGCA GAATCCAGCA GATACCTCAG GTTCAGAGGA AGCTGACACT
    CAGACTTTGT CATCTCAAGA TGATCAGCTG TCATATGAGG AACCTCAGCC GTCCACTTCC
    ACATCCAGTC TCTTCAGCAC ACCTACTACC TCTGCAAGTG AGCCAGCTTG CACTCTCTTG
    TATAAGTCCC AGGCATCAGT TCCTGGGGTA CAGCTTGAAA GTGGTGAGAA GTCTGGGGAA
    AGTCTTGAAG GACACAGCTC CACATCGGAT GCAACAGCAC ATGAGAAGGG ATCTTTAGGT
    CCTCCAAAAA AGAGAATGAG AACAGAGGAC CTCTGGACGG GCAACAAAAA TCTTGTTTCT
    GCAAGTTGTT CCAAAGGAGC AAGCGATGAA AGCAAGACAC CAAGTATGAA GCCAGATAAG
    ATGGAGGAAA CGCTCACCTG TATAATCTGT CAGGAATTGC TGCACGACTG TGTCAGTTTG
    CAGCCATGTA TGCACACTTT TTGTGCTGCT TGTTATTCTG GTTGGATGGA ACGCTCTTCC
    CTCTGTCCTA CCTGCCGCTG CCCTGTTGAA CGTATCTGTA AAAACCATAT ACTGAACAAT
    TTGGTGGAGG CCTATTTGAT CCAGCATCCA GAGAAATGCC GCAGTGAGGA AGACAGGTGT
    AGTATGGATG CACGGAATAA GATTACACAA GATATGTTGC AGCCAAAGGT ACGTCGGTCG
    TTTTCTGATG AAGAAGGGAG CTCTGAGGAT CTGCTGGAGC TTTCTGATGT GGATAGTGAA
    TCTTCTGATA TAAGCCAGCC TTATACTGTA TGCAGACAGT GCCCTGGATA TGTCCGCCAC
    AATATTCAAC CCCCACCATA TCCTCCACCT TCTGACACAG AGGCTTCACG TACTCAAGGA
    GATGCCCCTT CTACCTCTAC TAACTTTCCT ACAGCTACCC AGGAATATGT GTGCCCTTCT
    CATGGAAGCC ACGTGATCTG CACCTGCTGC TTCCAGCCGA TGCCAGACCG AAGGGCAGAA
    CGGGAGCACA ACTCTCATGT GGCACCTCAG CAATGCACTA TATGTTTGGA GCCCTTCTGT
    CACATGTACT GGGGATGCAA CCGGATGGGT TGTTTTGGGT GTTTAGCTCC ATTCTGTGAG
    TTGAATCTGG GAGACAAATG TTTGGATGGA GTTTTAAATA ACAATAACTA TGAATCTGAC
    ATCCTTAAGA ATTACCTGGC ATCCAGGGGA CTCACATGGA AAGATATGCT GAATGAAAGC
    TTGTCAGCTG CTCAGAGAGG GGTGTTTATG CTTCCTGATT ACAGGATAAA TGGAACCACT
    GTACTCTGCT ACTTCTGCGG GCTGCGGAAC TTTAGAATTC TAACATACCA GTACAGACAA
    AACATCCCAG CTTCAGAGCT ACCAGTGACT GTTACTTCTC GGCCAAATTG CTATTGGGGG
    CGGAATTGCA GAACTCAGGT TAAAGCTCAC CATGCTATGA AATTCAATCA CATATGTGAA
    CAAACCCGCT TCAAGAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa00419
    Clone ID Related Accession (Same CDS sequence) XM_012958547.2 , XM_012958542.3 , NM_204047.1 , XM_012958542.2 , XM_012958547.3
    Accession Version XM_012958547.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1881bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase CHFR isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012958547.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGATGGCT TAGGGGAGAA GAAGCCCTGG GGGAAGCTAA GTAGATTGTT GGGAGCTGAG 
    ACGGAATCCT CATCTGAACT GTTTCTCTAC AAAAAGGAAT GGACAATAGG CAGAAAGAAA
    GCTTGTGACC TGTCTTTCCC TGGAAACAAG TTAGTGTCTG GAGAACACTG CAAAATTACA
    GTGAATGAAG AATCTGGTAG TGTTTCCTTA GAGGACACCA GCACAAATGG CACAGTAATC
    AACAAGCTGA AAGTGGTAAA GAAGCAGACA TACCCCTTGA AAAATGGTGA TGTGATCTAT
    GTTGTTTATA GGAAAAATGA GCCAGAACAA AATGTTGCTT ACCTATACAA ATCCTTAAAC
    CAGGCAGAAG ATAGTATGCA GAATCCAGCA GATACCTCAG GTTCAGAGGA AGCTGACACT
    CAGACTTTGT CATCTCAAGA TGATCAGCTG TCATATGAGG AACCTCAGCC GTCCACTTCC
    ACATCCAGTC TCTTCAGCAC ACCTACTACC TCTGCAAGTG AGCCAGCTTG CACTCTCTTG
    TATAAGTCCC AGGCATCAGT TCCTGGGGTA CAGCTTGAAA GTGGTGAGAA GTCTGGGGAA
    AGTCTTGAAG GACACAGCTC CACATCGGAT GCAACAGCAC ATGAGAAGGG ATCTTTAGGT
    CCTCCAAAAA AGAGAATGAG AACAGAGGAC CTCTGGACGG GCAACAAAAA TCTTGTTTCT
    GCAAGTTGTT CCAAAGGAGC AAGCGATGAA AGCAAGACAC CAAGTATGAA GCCAGATAAG
    ATGGAGGAAA CGCTCACCTG TATAATCTGT CAGGAATTGC TGCACGACTG TGTCAGTTTG
    CAGCCATGTA TGCACACTTT TTGTGCTGCT TGTTATTCTG GTTGGATGGA ACGCTCTTCC
    CTCTGTCCTA CCTGCCGCTG CCCTGTTGAA CGTATCTGTA AAAACCATAT ACTGAACAAT
    TTGGTGGAGG CCTATTTGAT CCAGCATCCA GAGAAATGCC GCAGTGAGGA AGACAGGTGT
    AGTATGGATG CACGGAATAA GATTACACAA GATATGTTGC AGCCAAAGGT ACGTCGGTCG
    TTTTCTGATG AAGAAGGGAG CTCTGAGGAT CTGCTGGAGC TTTCTGATGT GGATAGTGAA
    TCTTCTGATA TAAGCCAGCC TTATACTGTA TGCAGACAGT GCCCTGGATA TGTCCGCCAC
    AATATTCAAC CCCCACCATA TCCTCCACCT TCTGACACAG AGGCTTCACG TACTCAAGGA
    GATGCCCCTT CTACCTCTAC TAACTTTCCT ACAGCTACCC AGGAATATGT GTGCCCTTCT
    CATGGAAGCC ACGTGATCTG CACCTGCTGC TTCCAGCCGA TGCCAGACCG AAGGGCAGAA
    CGGGAGCACA ACTCTCATGT GGCACCTCAG CAATGCACTA TATGTTTGGA GCCCTTCTGT
    CACATGTACT GGGGATGCAA CCGGATGGGT TGTTTTGGGT GTTTAGCTCC ATTCTGTGAG
    TTGAATCTGG GAGACAAATG TTTGGATGGA GTTTTAAATA ACAATAACTA TGAATCTGAC
    ATCCTTAAGA ATTACCTGGC ATCCAGGGGA CTCACATGGA AAGATATGCT GAATGAAAGC
    TTGTCAGCTG CTCAGAGAGG GGTGTTTATG CTTCCTGATT ACAGGATAAA TGGAACCACT
    GTACTCTGCT ACTTCTGCGG GCTGCGGAAC TTTAGAATTC TAACATACCA GTACAGACAA
    AACATCCCAG CTTCAGAGCT ACCAGTGACT GTTACTTCTC GGCCAAATTG CTATTGGGGG
    CGGAATTGCA GAACTCAGGT TAAAGCTCAC CATGCTATGA AATTCAATCA CATATGTGAA
    CAAACCCGCT TCAAGAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012814001.1
    CDS109..1989
    Translation

    Target ORF information:

    RefSeq Version XM_012958547.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase (chfr), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012958547.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGATGGCT TAGGGGAGAA GAAGCCCTGG GGGAAGCTAA GTAGATTGTT GGGAGCTGAG 
    ACGGAATCCT CATCTGAACT GTTTCTCTAC AAAAAGGAAT GGACAATAGG CAGAAAGAAA
    GCTTGTGACC TGTCTTTCCC TGGAAACAAG TTAGTGTCTG GAGAACACTG CAAAATTACA
    GTGAATGAAG AATCTGGTAG TGTTTCCTTA GAGGACACCA GCACAAATGG CACAGTAATC
    AACAAGCTGA AAGTGGTAAA GAAGCAGACA TACCCCTTGA AAAATGGTGA TGTGATCTAT
    GTTGTTTATA GGAAAAATGA GCCAGAACAA AATGTTGCTT ACCTATACAA ATCCTTAAAC
    CAGGCAGAAG ATAGTATGCA GAATCCAGCA GATACCTCAG GTTCAGAGGA AGCTGACACT
    CAGACTTTGT CATCTCAAGA TGATCAGCTG TCATATGAGG AACCTCAGCC GTCCACTTCC
    ACATCCAGTC TCTTCAGCAC ACCTACTACC TCTGCAAGTG AGCCAGCTTG CACTCTCTTG
    TATAAGTCCC AGGCATCAGT TCCTGGGGTA CAGCTTGAAA GTGGTGAGAA GTCTGGGGAA
    AGTCTTGAAG GACACAGCTC CACATCGGAT GCAACAGCAC ATGAGAAGGG ATCTTTAGGT
    CCTCCAAAAA AGAGAATGAG AACAGAGGAC CTCTGGACGG GCAACAAAAA TCTTGTTTCT
    GCAAGTTGTT CCAAAGGAGC AAGCGATGAA AGCAAGACAC CAAGTATGAA GCCAGATAAG
    ATGGAGGAAA CGCTCACCTG TATAATCTGT CAGGAATTGC TGCACGACTG TGTCAGTTTG
    CAGCCATGTA TGCACACTTT TTGTGCTGCT TGTTATTCTG GTTGGATGGA ACGCTCTTCC
    CTCTGTCCTA CCTGCCGCTG CCCTGTTGAA CGTATCTGTA AAAACCATAT ACTGAACAAT
    TTGGTGGAGG CCTATTTGAT CCAGCATCCA GAGAAATGCC GCAGTGAGGA AGACAGGTGT
    AGTATGGATG CACGGAATAA GATTACACAA GATATGTTGC AGCCAAAGGT ACGTCGGTCG
    TTTTCTGATG AAGAAGGGAG CTCTGAGGAT CTGCTGGAGC TTTCTGATGT GGATAGTGAA
    TCTTCTGATA TAAGCCAGCC TTATACTGTA TGCAGACAGT GCCCTGGATA TGTCCGCCAC
    AATATTCAAC CCCCACCATA TCCTCCACCT TCTGACACAG AGGCTTCACG TACTCAAGGA
    GATGCCCCTT CTACCTCTAC TAACTTTCCT ACAGCTACCC AGGAATATGT GTGCCCTTCT
    CATGGAAGCC ACGTGATCTG CACCTGCTGC TTCCAGCCGA TGCCAGACCG AAGGGCAGAA
    CGGGAGCACA ACTCTCATGT GGCACCTCAG CAATGCACTA TATGTTTGGA GCCCTTCTGT
    CACATGTACT GGGGATGCAA CCGGATGGGT TGTTTTGGGT GTTTAGCTCC ATTCTGTGAG
    TTGAATCTGG GAGACAAATG TTTGGATGGA GTTTTAAATA ACAATAACTA TGAATCTGAC
    ATCCTTAAGA ATTACCTGGC ATCCAGGGGA CTCACATGGA AAGATATGCT GAATGAAAGC
    TTGTCAGCTG CTCAGAGAGG GGTGTTTATG CTTCCTGATT ACAGGATAAA TGGAACCACT
    GTACTCTGCT ACTTCTGCGG GCTGCGGAAC TTTAGAATTC TAACATACCA GTACAGACAA
    AACATCCCAG CTTCAGAGCT ACCAGTGACT GTTACTTCTC GGCCAAATTG CTATTGGGGG
    CGGAATTGCA GAACTCAGGT TAAAGCTCAC CATGCTATGA AATTCAATCA CATATGTGAA
    CAAACCCGCT TCAAGAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa00419
    Clone ID Related Accession (Same CDS sequence) XM_012958547.2 , XM_012958542.3 , NM_204047.1 , XM_012958542.2 , XM_012958547.3
    Accession Version XM_012958542.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1881bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase CHFR isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012958542.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGATGGCT TAGGGGAGAA GAAGCCCTGG GGGAAGCTAA GTAGATTGTT GGGAGCTGAG 
    ACGGAATCCT CATCTGAACT GTTTCTCTAC AAAAAGGAAT GGACAATAGG CAGAAAGAAA
    GCTTGTGACC TGTCTTTCCC TGGAAACAAG TTAGTGTCTG GAGAACACTG CAAAATTACA
    GTGAATGAAG AATCTGGTAG TGTTTCCTTA GAGGACACCA GCACAAATGG CACAGTAATC
    AACAAGCTGA AAGTGGTAAA GAAGCAGACA TACCCCTTGA AAAATGGTGA TGTGATCTAT
    GTTGTTTATA GGAAAAATGA GCCAGAACAA AATGTTGCTT ACCTATACAA ATCCTTAAAC
    CAGGCAGAAG ATAGTATGCA GAATCCAGCA GATACCTCAG GTTCAGAGGA AGCTGACACT
    CAGACTTTGT CATCTCAAGA TGATCAGCTG TCATATGAGG AACCTCAGCC GTCCACTTCC
    ACATCCAGTC TCTTCAGCAC ACCTACTACC TCTGCAAGTG AGCCAGCTTG CACTCTCTTG
    TATAAGTCCC AGGCATCAGT TCCTGGGGTA CAGCTTGAAA GTGGTGAGAA GTCTGGGGAA
    AGTCTTGAAG GACACAGCTC CACATCGGAT GCAACAGCAC ATGAGAAGGG ATCTTTAGGT
    CCTCCAAAAA AGAGAATGAG AACAGAGGAC CTCTGGACGG GCAACAAAAA TCTTGTTTCT
    GCAAGTTGTT CCAAAGGAGC AAGCGATGAA AGCAAGACAC CAAGTATGAA GCCAGATAAG
    ATGGAGGAAA CGCTCACCTG TATAATCTGT CAGGAATTGC TGCACGACTG TGTCAGTTTG
    CAGCCATGTA TGCACACTTT TTGTGCTGCT TGTTATTCTG GTTGGATGGA ACGCTCTTCC
    CTCTGTCCTA CCTGCCGCTG CCCTGTTGAA CGTATCTGTA AAAACCATAT ACTGAACAAT
    TTGGTGGAGG CCTATTTGAT CCAGCATCCA GAGAAATGCC GCAGTGAGGA AGACAGGTGT
    AGTATGGATG CACGGAATAA GATTACACAA GATATGTTGC AGCCAAAGGT ACGTCGGTCG
    TTTTCTGATG AAGAAGGGAG CTCTGAGGAT CTGCTGGAGC TTTCTGATGT GGATAGTGAA
    TCTTCTGATA TAAGCCAGCC TTATACTGTA TGCAGACAGT GCCCTGGATA TGTCCGCCAC
    AATATTCAAC CCCCACCATA TCCTCCACCT TCTGACACAG AGGCTTCACG TACTCAAGGA
    GATGCCCCTT CTACCTCTAC TAACTTTCCT ACAGCTACCC AGGAATATGT GTGCCCTTCT
    CATGGAAGCC ACGTGATCTG CACCTGCTGC TTCCAGCCGA TGCCAGACCG AAGGGCAGAA
    CGGGAGCACA ACTCTCATGT GGCACCTCAG CAATGCACTA TATGTTTGGA GCCCTTCTGT
    CACATGTACT GGGGATGCAA CCGGATGGGT TGTTTTGGGT GTTTAGCTCC ATTCTGTGAG
    TTGAATCTGG GAGACAAATG TTTGGATGGA GTTTTAAATA ACAATAACTA TGAATCTGAC
    ATCCTTAAGA ATTACCTGGC ATCCAGGGGA CTCACATGGA AAGATATGCT GAATGAAAGC
    TTGTCAGCTG CTCAGAGAGG GGTGTTTATG CTTCCTGATT ACAGGATAAA TGGAACCACT
    GTACTCTGCT ACTTCTGCGG GCTGCGGAAC TTTAGAATTC TAACATACCA GTACAGACAA
    AACATCCCAG CTTCAGAGCT ACCAGTGACT GTTACTTCTC GGCCAAATTG CTATTGGGGG
    CGGAATTGCA GAACTCAGGT TAAAGCTCAC CATGCTATGA AATTCAATCA CATATGTGAA
    CAAACCCGCT TCAAGAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012813996.1
    CDS203..2083
    Translation

    Target ORF information:

    RefSeq Version XM_012958542.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase (chfr), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012958542.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGATGGCT TAGGGGAGAA GAAGCCCTGG GGGAAGCTAA GTAGATTGTT GGGAGCTGAG 
    ACGGAATCCT CATCTGAACT GTTTCTCTAC AAAAAGGAAT GGACAATAGG CAGAAAGAAA
    GCTTGTGACC TGTCTTTCCC TGGAAACAAG TTAGTGTCTG GAGAACACTG CAAAATTACA
    GTGAATGAAG AATCTGGTAG TGTTTCCTTA GAGGACACCA GCACAAATGG CACAGTAATC
    AACAAGCTGA AAGTGGTAAA GAAGCAGACA TACCCCTTGA AAAATGGTGA TGTGATCTAT
    GTTGTTTATA GGAAAAATGA GCCAGAACAA AATGTTGCTT ACCTATACAA ATCCTTAAAC
    CAGGCAGAAG ATAGTATGCA GAATCCAGCA GATACCTCAG GTTCAGAGGA AGCTGACACT
    CAGACTTTGT CATCTCAAGA TGATCAGCTG TCATATGAGG AACCTCAGCC GTCCACTTCC
    ACATCCAGTC TCTTCAGCAC ACCTACTACC TCTGCAAGTG AGCCAGCTTG CACTCTCTTG
    TATAAGTCCC AGGCATCAGT TCCTGGGGTA CAGCTTGAAA GTGGTGAGAA GTCTGGGGAA
    AGTCTTGAAG GACACAGCTC CACATCGGAT GCAACAGCAC ATGAGAAGGG ATCTTTAGGT
    CCTCCAAAAA AGAGAATGAG AACAGAGGAC CTCTGGACGG GCAACAAAAA TCTTGTTTCT
    GCAAGTTGTT CCAAAGGAGC AAGCGATGAA AGCAAGACAC CAAGTATGAA GCCAGATAAG
    ATGGAGGAAA CGCTCACCTG TATAATCTGT CAGGAATTGC TGCACGACTG TGTCAGTTTG
    CAGCCATGTA TGCACACTTT TTGTGCTGCT TGTTATTCTG GTTGGATGGA ACGCTCTTCC
    CTCTGTCCTA CCTGCCGCTG CCCTGTTGAA CGTATCTGTA AAAACCATAT ACTGAACAAT
    TTGGTGGAGG CCTATTTGAT CCAGCATCCA GAGAAATGCC GCAGTGAGGA AGACAGGTGT
    AGTATGGATG CACGGAATAA GATTACACAA GATATGTTGC AGCCAAAGGT ACGTCGGTCG
    TTTTCTGATG AAGAAGGGAG CTCTGAGGAT CTGCTGGAGC TTTCTGATGT GGATAGTGAA
    TCTTCTGATA TAAGCCAGCC TTATACTGTA TGCAGACAGT GCCCTGGATA TGTCCGCCAC
    AATATTCAAC CCCCACCATA TCCTCCACCT TCTGACACAG AGGCTTCACG TACTCAAGGA
    GATGCCCCTT CTACCTCTAC TAACTTTCCT ACAGCTACCC AGGAATATGT GTGCCCTTCT
    CATGGAAGCC ACGTGATCTG CACCTGCTGC TTCCAGCCGA TGCCAGACCG AAGGGCAGAA
    CGGGAGCACA ACTCTCATGT GGCACCTCAG CAATGCACTA TATGTTTGGA GCCCTTCTGT
    CACATGTACT GGGGATGCAA CCGGATGGGT TGTTTTGGGT GTTTAGCTCC ATTCTGTGAG
    TTGAATCTGG GAGACAAATG TTTGGATGGA GTTTTAAATA ACAATAACTA TGAATCTGAC
    ATCCTTAAGA ATTACCTGGC ATCCAGGGGA CTCACATGGA AAGATATGCT GAATGAAAGC
    TTGTCAGCTG CTCAGAGAGG GGTGTTTATG CTTCCTGATT ACAGGATAAA TGGAACCACT
    GTACTCTGCT ACTTCTGCGG GCTGCGGAAC TTTAGAATTC TAACATACCA GTACAGACAA
    AACATCCCAG CTTCAGAGCT ACCAGTGACT GTTACTTCTC GGCCAAATTG CTATTGGGGG
    CGGAATTGCA GAACTCAGGT TAAAGCTCAC CATGCTATGA AATTCAATCA CATATGTGAA
    CAAACCCGCT TCAAGAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa00419
    Clone ID Related Accession (Same CDS sequence) XM_012958547.2 , XM_012958542.3 , NM_204047.1 , XM_012958542.2 , XM_012958547.3
    Accession Version XM_012958542.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1881bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase CHFR isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012958542.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGATGGCT TAGGGGAGAA GAAGCCCTGG GGGAAGCTAA GTAGATTGTT GGGAGCTGAG 
    ACGGAATCCT CATCTGAACT GTTTCTCTAC AAAAAGGAAT GGACAATAGG CAGAAAGAAA
    GCTTGTGACC TGTCTTTCCC TGGAAACAAG TTAGTGTCTG GAGAACACTG CAAAATTACA
    GTGAATGAAG AATCTGGTAG TGTTTCCTTA GAGGACACCA GCACAAATGG CACAGTAATC
    AACAAGCTGA AAGTGGTAAA GAAGCAGACA TACCCCTTGA AAAATGGTGA TGTGATCTAT
    GTTGTTTATA GGAAAAATGA GCCAGAACAA AATGTTGCTT ACCTATACAA ATCCTTAAAC
    CAGGCAGAAG ATAGTATGCA GAATCCAGCA GATACCTCAG GTTCAGAGGA AGCTGACACT
    CAGACTTTGT CATCTCAAGA TGATCAGCTG TCATATGAGG AACCTCAGCC GTCCACTTCC
    ACATCCAGTC TCTTCAGCAC ACCTACTACC TCTGCAAGTG AGCCAGCTTG CACTCTCTTG
    TATAAGTCCC AGGCATCAGT TCCTGGGGTA CAGCTTGAAA GTGGTGAGAA GTCTGGGGAA
    AGTCTTGAAG GACACAGCTC CACATCGGAT GCAACAGCAC ATGAGAAGGG ATCTTTAGGT
    CCTCCAAAAA AGAGAATGAG AACAGAGGAC CTCTGGACGG GCAACAAAAA TCTTGTTTCT
    GCAAGTTGTT CCAAAGGAGC AAGCGATGAA AGCAAGACAC CAAGTATGAA GCCAGATAAG
    ATGGAGGAAA CGCTCACCTG TATAATCTGT CAGGAATTGC TGCACGACTG TGTCAGTTTG
    CAGCCATGTA TGCACACTTT TTGTGCTGCT TGTTATTCTG GTTGGATGGA ACGCTCTTCC
    CTCTGTCCTA CCTGCCGCTG CCCTGTTGAA CGTATCTGTA AAAACCATAT ACTGAACAAT
    TTGGTGGAGG CCTATTTGAT CCAGCATCCA GAGAAATGCC GCAGTGAGGA AGACAGGTGT
    AGTATGGATG CACGGAATAA GATTACACAA GATATGTTGC AGCCAAAGGT ACGTCGGTCG
    TTTTCTGATG AAGAAGGGAG CTCTGAGGAT CTGCTGGAGC TTTCTGATGT GGATAGTGAA
    TCTTCTGATA TAAGCCAGCC TTATACTGTA TGCAGACAGT GCCCTGGATA TGTCCGCCAC
    AATATTCAAC CCCCACCATA TCCTCCACCT TCTGACACAG AGGCTTCACG TACTCAAGGA
    GATGCCCCTT CTACCTCTAC TAACTTTCCT ACAGCTACCC AGGAATATGT GTGCCCTTCT
    CATGGAAGCC ACGTGATCTG CACCTGCTGC TTCCAGCCGA TGCCAGACCG AAGGGCAGAA
    CGGGAGCACA ACTCTCATGT GGCACCTCAG CAATGCACTA TATGTTTGGA GCCCTTCTGT
    CACATGTACT GGGGATGCAA CCGGATGGGT TGTTTTGGGT GTTTAGCTCC ATTCTGTGAG
    TTGAATCTGG GAGACAAATG TTTGGATGGA GTTTTAAATA ACAATAACTA TGAATCTGAC
    ATCCTTAAGA ATTACCTGGC ATCCAGGGGA CTCACATGGA AAGATATGCT GAATGAAAGC
    TTGTCAGCTG CTCAGAGAGG GGTGTTTATG CTTCCTGATT ACAGGATAAA TGGAACCACT
    GTACTCTGCT ACTTCTGCGG GCTGCGGAAC TTTAGAATTC TAACATACCA GTACAGACAA
    AACATCCCAG CTTCAGAGCT ACCAGTGACT GTTACTTCTC GGCCAAATTG CTATTGGGGG
    CGGAATTGCA GAACTCAGGT TAAAGCTCAC CATGCTATGA AATTCAATCA CATATGTGAA
    CAAACCCGCT TCAAGAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012813996.1
    CDS190..2070
    Translation

    Target ORF information:

    RefSeq Version XM_012958542.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase (chfr), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012958542.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGATGGCT TAGGGGAGAA GAAGCCCTGG GGGAAGCTAA GTAGATTGTT GGGAGCTGAG 
    ACGGAATCCT CATCTGAACT GTTTCTCTAC AAAAAGGAAT GGACAATAGG CAGAAAGAAA
    GCTTGTGACC TGTCTTTCCC TGGAAACAAG TTAGTGTCTG GAGAACACTG CAAAATTACA
    GTGAATGAAG AATCTGGTAG TGTTTCCTTA GAGGACACCA GCACAAATGG CACAGTAATC
    AACAAGCTGA AAGTGGTAAA GAAGCAGACA TACCCCTTGA AAAATGGTGA TGTGATCTAT
    GTTGTTTATA GGAAAAATGA GCCAGAACAA AATGTTGCTT ACCTATACAA ATCCTTAAAC
    CAGGCAGAAG ATAGTATGCA GAATCCAGCA GATACCTCAG GTTCAGAGGA AGCTGACACT
    CAGACTTTGT CATCTCAAGA TGATCAGCTG TCATATGAGG AACCTCAGCC GTCCACTTCC
    ACATCCAGTC TCTTCAGCAC ACCTACTACC TCTGCAAGTG AGCCAGCTTG CACTCTCTTG
    TATAAGTCCC AGGCATCAGT TCCTGGGGTA CAGCTTGAAA GTGGTGAGAA GTCTGGGGAA
    AGTCTTGAAG GACACAGCTC CACATCGGAT GCAACAGCAC ATGAGAAGGG ATCTTTAGGT
    CCTCCAAAAA AGAGAATGAG AACAGAGGAC CTCTGGACGG GCAACAAAAA TCTTGTTTCT
    GCAAGTTGTT CCAAAGGAGC AAGCGATGAA AGCAAGACAC CAAGTATGAA GCCAGATAAG
    ATGGAGGAAA CGCTCACCTG TATAATCTGT CAGGAATTGC TGCACGACTG TGTCAGTTTG
    CAGCCATGTA TGCACACTTT TTGTGCTGCT TGTTATTCTG GTTGGATGGA ACGCTCTTCC
    CTCTGTCCTA CCTGCCGCTG CCCTGTTGAA CGTATCTGTA AAAACCATAT ACTGAACAAT
    TTGGTGGAGG CCTATTTGAT CCAGCATCCA GAGAAATGCC GCAGTGAGGA AGACAGGTGT
    AGTATGGATG CACGGAATAA GATTACACAA GATATGTTGC AGCCAAAGGT ACGTCGGTCG
    TTTTCTGATG AAGAAGGGAG CTCTGAGGAT CTGCTGGAGC TTTCTGATGT GGATAGTGAA
    TCTTCTGATA TAAGCCAGCC TTATACTGTA TGCAGACAGT GCCCTGGATA TGTCCGCCAC
    AATATTCAAC CCCCACCATA TCCTCCACCT TCTGACACAG AGGCTTCACG TACTCAAGGA
    GATGCCCCTT CTACCTCTAC TAACTTTCCT ACAGCTACCC AGGAATATGT GTGCCCTTCT
    CATGGAAGCC ACGTGATCTG CACCTGCTGC TTCCAGCCGA TGCCAGACCG AAGGGCAGAA
    CGGGAGCACA ACTCTCATGT GGCACCTCAG CAATGCACTA TATGTTTGGA GCCCTTCTGT
    CACATGTACT GGGGATGCAA CCGGATGGGT TGTTTTGGGT GTTTAGCTCC ATTCTGTGAG
    TTGAATCTGG GAGACAAATG TTTGGATGGA GTTTTAAATA ACAATAACTA TGAATCTGAC
    ATCCTTAAGA ATTACCTGGC ATCCAGGGGA CTCACATGGA AAGATATGCT GAATGAAAGC
    TTGTCAGCTG CTCAGAGAGG GGTGTTTATG CTTCCTGATT ACAGGATAAA TGGAACCACT
    GTACTCTGCT ACTTCTGCGG GCTGCGGAAC TTTAGAATTC TAACATACCA GTACAGACAA
    AACATCCCAG CTTCAGAGCT ACCAGTGACT GTTACTTCTC GGCCAAATTG CTATTGGGGG
    CGGAATTGCA GAACTCAGGT TAAAGCTCAC CATGCTATGA AATTCAATCA CATATGTGAA
    CAAACCCGCT TCAAGAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa00419
    Clone ID Related Accession (Same CDS sequence) XM_012958547.2 , XM_012958542.3 , NM_204047.1 , XM_012958542.2 , XM_012958547.3
    Accession Version XM_012958547.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1881bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase CHFR isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012958547.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGATGGCT TAGGGGAGAA GAAGCCCTGG GGGAAGCTAA GTAGATTGTT GGGAGCTGAG 
    ACGGAATCCT CATCTGAACT GTTTCTCTAC AAAAAGGAAT GGACAATAGG CAGAAAGAAA
    GCTTGTGACC TGTCTTTCCC TGGAAACAAG TTAGTGTCTG GAGAACACTG CAAAATTACA
    GTGAATGAAG AATCTGGTAG TGTTTCCTTA GAGGACACCA GCACAAATGG CACAGTAATC
    AACAAGCTGA AAGTGGTAAA GAAGCAGACA TACCCCTTGA AAAATGGTGA TGTGATCTAT
    GTTGTTTATA GGAAAAATGA GCCAGAACAA AATGTTGCTT ACCTATACAA ATCCTTAAAC
    CAGGCAGAAG ATAGTATGCA GAATCCAGCA GATACCTCAG GTTCAGAGGA AGCTGACACT
    CAGACTTTGT CATCTCAAGA TGATCAGCTG TCATATGAGG AACCTCAGCC GTCCACTTCC
    ACATCCAGTC TCTTCAGCAC ACCTACTACC TCTGCAAGTG AGCCAGCTTG CACTCTCTTG
    TATAAGTCCC AGGCATCAGT TCCTGGGGTA CAGCTTGAAA GTGGTGAGAA GTCTGGGGAA
    AGTCTTGAAG GACACAGCTC CACATCGGAT GCAACAGCAC ATGAGAAGGG ATCTTTAGGT
    CCTCCAAAAA AGAGAATGAG AACAGAGGAC CTCTGGACGG GCAACAAAAA TCTTGTTTCT
    GCAAGTTGTT CCAAAGGAGC AAGCGATGAA AGCAAGACAC CAAGTATGAA GCCAGATAAG
    ATGGAGGAAA CGCTCACCTG TATAATCTGT CAGGAATTGC TGCACGACTG TGTCAGTTTG
    CAGCCATGTA TGCACACTTT TTGTGCTGCT TGTTATTCTG GTTGGATGGA ACGCTCTTCC
    CTCTGTCCTA CCTGCCGCTG CCCTGTTGAA CGTATCTGTA AAAACCATAT ACTGAACAAT
    TTGGTGGAGG CCTATTTGAT CCAGCATCCA GAGAAATGCC GCAGTGAGGA AGACAGGTGT
    AGTATGGATG CACGGAATAA GATTACACAA GATATGTTGC AGCCAAAGGT ACGTCGGTCG
    TTTTCTGATG AAGAAGGGAG CTCTGAGGAT CTGCTGGAGC TTTCTGATGT GGATAGTGAA
    TCTTCTGATA TAAGCCAGCC TTATACTGTA TGCAGACAGT GCCCTGGATA TGTCCGCCAC
    AATATTCAAC CCCCACCATA TCCTCCACCT TCTGACACAG AGGCTTCACG TACTCAAGGA
    GATGCCCCTT CTACCTCTAC TAACTTTCCT ACAGCTACCC AGGAATATGT GTGCCCTTCT
    CATGGAAGCC ACGTGATCTG CACCTGCTGC TTCCAGCCGA TGCCAGACCG AAGGGCAGAA
    CGGGAGCACA ACTCTCATGT GGCACCTCAG CAATGCACTA TATGTTTGGA GCCCTTCTGT
    CACATGTACT GGGGATGCAA CCGGATGGGT TGTTTTGGGT GTTTAGCTCC ATTCTGTGAG
    TTGAATCTGG GAGACAAATG TTTGGATGGA GTTTTAAATA ACAATAACTA TGAATCTGAC
    ATCCTTAAGA ATTACCTGGC ATCCAGGGGA CTCACATGGA AAGATATGCT GAATGAAAGC
    TTGTCAGCTG CTCAGAGAGG GGTGTTTATG CTTCCTGATT ACAGGATAAA TGGAACCACT
    GTACTCTGCT ACTTCTGCGG GCTGCGGAAC TTTAGAATTC TAACATACCA GTACAGACAA
    AACATCCCAG CTTCAGAGCT ACCAGTGACT GTTACTTCTC GGCCAAATTG CTATTGGGGG
    CGGAATTGCA GAACTCAGGT TAAAGCTCAC CATGCTATGA AATTCAATCA CATATGTGAA
    CAAACCCGCT TCAAGAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012814001.1
    CDS117..1997
    Translation

    Target ORF information:

    RefSeq Version XM_012958547.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase (chfr), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012958547.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGATGGCT TAGGGGAGAA GAAGCCCTGG GGGAAGCTAA GTAGATTGTT GGGAGCTGAG 
    ACGGAATCCT CATCTGAACT GTTTCTCTAC AAAAAGGAAT GGACAATAGG CAGAAAGAAA
    GCTTGTGACC TGTCTTTCCC TGGAAACAAG TTAGTGTCTG GAGAACACTG CAAAATTACA
    GTGAATGAAG AATCTGGTAG TGTTTCCTTA GAGGACACCA GCACAAATGG CACAGTAATC
    AACAAGCTGA AAGTGGTAAA GAAGCAGACA TACCCCTTGA AAAATGGTGA TGTGATCTAT
    GTTGTTTATA GGAAAAATGA GCCAGAACAA AATGTTGCTT ACCTATACAA ATCCTTAAAC
    CAGGCAGAAG ATAGTATGCA GAATCCAGCA GATACCTCAG GTTCAGAGGA AGCTGACACT
    CAGACTTTGT CATCTCAAGA TGATCAGCTG TCATATGAGG AACCTCAGCC GTCCACTTCC
    ACATCCAGTC TCTTCAGCAC ACCTACTACC TCTGCAAGTG AGCCAGCTTG CACTCTCTTG
    TATAAGTCCC AGGCATCAGT TCCTGGGGTA CAGCTTGAAA GTGGTGAGAA GTCTGGGGAA
    AGTCTTGAAG GACACAGCTC CACATCGGAT GCAACAGCAC ATGAGAAGGG ATCTTTAGGT
    CCTCCAAAAA AGAGAATGAG AACAGAGGAC CTCTGGACGG GCAACAAAAA TCTTGTTTCT
    GCAAGTTGTT CCAAAGGAGC AAGCGATGAA AGCAAGACAC CAAGTATGAA GCCAGATAAG
    ATGGAGGAAA CGCTCACCTG TATAATCTGT CAGGAATTGC TGCACGACTG TGTCAGTTTG
    CAGCCATGTA TGCACACTTT TTGTGCTGCT TGTTATTCTG GTTGGATGGA ACGCTCTTCC
    CTCTGTCCTA CCTGCCGCTG CCCTGTTGAA CGTATCTGTA AAAACCATAT ACTGAACAAT
    TTGGTGGAGG CCTATTTGAT CCAGCATCCA GAGAAATGCC GCAGTGAGGA AGACAGGTGT
    AGTATGGATG CACGGAATAA GATTACACAA GATATGTTGC AGCCAAAGGT ACGTCGGTCG
    TTTTCTGATG AAGAAGGGAG CTCTGAGGAT CTGCTGGAGC TTTCTGATGT GGATAGTGAA
    TCTTCTGATA TAAGCCAGCC TTATACTGTA TGCAGACAGT GCCCTGGATA TGTCCGCCAC
    AATATTCAAC CCCCACCATA TCCTCCACCT TCTGACACAG AGGCTTCACG TACTCAAGGA
    GATGCCCCTT CTACCTCTAC TAACTTTCCT ACAGCTACCC AGGAATATGT GTGCCCTTCT
    CATGGAAGCC ACGTGATCTG CACCTGCTGC TTCCAGCCGA TGCCAGACCG AAGGGCAGAA
    CGGGAGCACA ACTCTCATGT GGCACCTCAG CAATGCACTA TATGTTTGGA GCCCTTCTGT
    CACATGTACT GGGGATGCAA CCGGATGGGT TGTTTTGGGT GTTTAGCTCC ATTCTGTGAG
    TTGAATCTGG GAGACAAATG TTTGGATGGA GTTTTAAATA ACAATAACTA TGAATCTGAC
    ATCCTTAAGA ATTACCTGGC ATCCAGGGGA CTCACATGGA AAGATATGCT GAATGAAAGC
    TTGTCAGCTG CTCAGAGAGG GGTGTTTATG CTTCCTGATT ACAGGATAAA TGGAACCACT
    GTACTCTGCT ACTTCTGCGG GCTGCGGAAC TTTAGAATTC TAACATACCA GTACAGACAA
    AACATCCCAG CTTCAGAGCT ACCAGTGACT GTTACTTCTC GGCCAAATTG CTATTGGGGG
    CGGAATTGCA GAACTCAGGT TAAAGCTCAC CATGCTATGA AATTCAATCA CATATGTGAA
    CAAACCCGCT TCAAGAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa41728
    Clone ID Related Accession (Same CDS sequence) XM_018091888.1
    Accession Version XM_018091888.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1683bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase CHFR isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGACAATA GGCAGAAAGA AAGCACAAAT GGCACAGTAA TCAACAAGCT GAAAGTGGTA 
    AAGAAGCAGA CATACCCCTT GAAAAATGGT GATGTGATCT ATGTTGTTTA TAGGAAAAAT
    GAGCCAGAAC AAAATGTTGC TTACCTATAC AAATCCTTAA ACCAGGCAGA AGATAGTATG
    CAGAATCCAG CAGATACCTC AGGTTCAGAG GAAGCTGACA CTCAGACTTT GTCATCTCAA
    GATGATCAGC TGTCATATGA GGAACCTCAG CCGTCCACTT CCACATCCAG TCTCTTCAGC
    ACACCTACTA CCTCTGCAAG TGAGCCAGCT TGCACTCTCT TGTATAAGTC CCAGGCATCA
    GTTCCTGGGG TACAGCTTGA AAGTGGTGAG AAGTCTGGGG AAAGTCTTGA AGGACACAGC
    TCCACATCGG ATGCAACAGC ACATGAGAAG GGATCTTTAG GTCCTCCAAA AAAGAGAATG
    AGAACAGAGG ACCTCTGGAC GGGCAACAAA AATCTTGTTT CTGCAAGTTG TTCCAAAGGA
    GCAAGCGATG AAAGCAAGAC ACCAAGTATG AAGCCAGATA AGATGGAGGA AACGCTCACC
    TGTATAATCT GTCAGGAATT GCTGCACGAC TGTGTCAGTT TGCAGCCATG TATGCACACT
    TTTTGTGCTG CTTGTTATTC TGGTTGGATG GAACGCTCTT CCCTCTGTCC TACCTGCCGC
    TGCCCTGTTG AACGTATCTG TAAAAACCAT ATACTGAACA ATTTGGTGGA GGCCTATTTG
    ATCCAGCATC CAGAGAAATG CCGCAGTGAG GAAGACAGGT GTAGTATGGA TGCACGGAAT
    AAGATTACAC AAGATATGTT GCAGCCAAAG GTACGTCGGT CGTTTTCTGA TGAAGAAGGG
    AGCTCTGAGG ATCTGCTGGA GCTTTCTGAT GTGGATAGTG AATCTTCTGA TATAAGCCAG
    CCTTATACTG TATGCAGACA GTGCCCTGGA TATGTCCGCC ACAATATTCA ACCCCCACCA
    TATCCTCCAC CTTCTGACAC AGAGGCTTCA CGTACTCAAG GAGATGCCCC TTCTACCTCT
    ACTAACTTTC CTACAGCTAC CCAGGAATAT GTGTGCCCTT CTCATGGAAG CCACGTGATC
    TGCACCTGCT GCTTCCAGCC GATGCCAGAC CGAAGGGCAG AACGGGAGCA CAACTCTCAT
    GTGGCACCTC AGCAATGCAC TATATGTTTG GAGCCCTTCT GTCACATGTA CTGGGGATGC
    AACCGGATGG GTTGTTTTGG GTGTTTAGCT CCATTCTGTG AGTTGAATCT GGGAGACAAA
    TGTTTGGATG GAGTTTTAAA TAACAATAAC TATGAATCTG ACATCCTTAA GAATTACCTG
    GCATCCAGGG GACTCACATG GAAAGATATG CTGAATGAAA GCTTGTCAGC TGCTCAGAGA
    GGGGTGTTTA TGCTTCCTGA TTACAGGATA AATGGAACCA CTGTACTCTG CTACTTCTGC
    GGGCTGCGGA ACTTTAGAAT TCTAACATAC CAGTACAGAC AAAACATCCC AGCTTCAGAG
    CTACCAGTGA CTGTTACTTC TCGGCCAAAT TGCTATTGGG GGCGGAATTG CAGAACTCAG
    GTTAAAGCTC ACCATGCTAT GAAATTCAAT CACATATGTG AACAAACCCG CTTCAAGAAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017947377.1
    CDS303..1985
    Translation

    Target ORF information:

    RefSeq Version XM_018091888.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase (chfr), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018091888.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGACAATA GGCAGAAAGA AAGCACAAAT GGCACAGTAA TCAACAAGCT GAAAGTGGTA 
    AAGAAGCAGA CATACCCCTT GAAAAATGGT GATGTGATCT ATGTTGTTTA TAGGAAAAAT
    GAGCCAGAAC AAAATGTTGC TTACCTATAC AAATCCTTAA ACCAGGCAGA AGATAGTATG
    CAGAATCCAG CAGATACCTC AGGTTCAGAG GAAGCTGACA CTCAGACTTT GTCATCTCAA
    GATGATCAGC TGTCATATGA GGAACCTCAG CCGTCCACTT CCACATCCAG TCTCTTCAGC
    ACACCTACTA CCTCTGCAAG TGAGCCAGCT TGCACTCTCT TGTATAAGTC CCAGGCATCA
    GTTCCTGGGG TACAGCTTGA AAGTGGTGAG AAGTCTGGGG AAAGTCTTGA AGGACACAGC
    TCCACATCGG ATGCAACAGC ACATGAGAAG GGATCTTTAG GTCCTCCAAA AAAGAGAATG
    AGAACAGAGG ACCTCTGGAC GGGCAACAAA AATCTTGTTT CTGCAAGTTG TTCCAAAGGA
    GCAAGCGATG AAAGCAAGAC ACCAAGTATG AAGCCAGATA AGATGGAGGA AACGCTCACC
    TGTATAATCT GTCAGGAATT GCTGCACGAC TGTGTCAGTT TGCAGCCATG TATGCACACT
    TTTTGTGCTG CTTGTTATTC TGGTTGGATG GAACGCTCTT CCCTCTGTCC TACCTGCCGC
    TGCCCTGTTG AACGTATCTG TAAAAACCAT ATACTGAACA ATTTGGTGGA GGCCTATTTG
    ATCCAGCATC CAGAGAAATG CCGCAGTGAG GAAGACAGGT GTAGTATGGA TGCACGGAAT
    AAGATTACAC AAGATATGTT GCAGCCAAAG GTACGTCGGT CGTTTTCTGA TGAAGAAGGG
    AGCTCTGAGG ATCTGCTGGA GCTTTCTGAT GTGGATAGTG AATCTTCTGA TATAAGCCAG
    CCTTATACTG TATGCAGACA GTGCCCTGGA TATGTCCGCC ACAATATTCA ACCCCCACCA
    TATCCTCCAC CTTCTGACAC AGAGGCTTCA CGTACTCAAG GAGATGCCCC TTCTACCTCT
    ACTAACTTTC CTACAGCTAC CCAGGAATAT GTGTGCCCTT CTCATGGAAG CCACGTGATC
    TGCACCTGCT GCTTCCAGCC GATGCCAGAC CGAAGGGCAG AACGGGAGCA CAACTCTCAT
    GTGGCACCTC AGCAATGCAC TATATGTTTG GAGCCCTTCT GTCACATGTA CTGGGGATGC
    AACCGGATGG GTTGTTTTGG GTGTTTAGCT CCATTCTGTG AGTTGAATCT GGGAGACAAA
    TGTTTGGATG GAGTTTTAAA TAACAATAAC TATGAATCTG ACATCCTTAA GAATTACCTG
    GCATCCAGGG GACTCACATG GAAAGATATG CTGAATGAAA GCTTGTCAGC TGCTCAGAGA
    GGGGTGTTTA TGCTTCCTGA TTACAGGATA AATGGAACCA CTGTACTCTG CTACTTCTGC
    GGGCTGCGGA ACTTTAGAAT TCTAACATAC CAGTACAGAC AAAACATCCC AGCTTCAGAG
    CTACCAGTGA CTGTTACTTC TCGGCCAAAT TGCTATTGGG GGCGGAATTG CAGAACTCAG
    GTTAAAGCTC ACCATGCTAT GAAATTCAAT CACATATGTG AACAAACCCG CTTCAAGAAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.