SMG7 gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_010813528.3	XP_010811830.2	protein SMG7 isoform X7
XM_015475257.1	XP_015330743.1	protein SMG7 isoform X4
XM_015475258.1	XP_015330744.1	protein SMG7 isoform X5
XM_010813528.2	XP_010811830.2	protein SMG7 isoform X6
XM_005217296.4	XP_005217353.1	protein SMG7 isoform X1
XM_005217297.4	XP_005217354.1	protein SMG7 isoform X2
XM_010813526.3	XP_010811828.2	protein SMG7 isoform X3
XM_024976351.1	XP_024832119.1	protein SMG7 isoform X10
XM_024976352.1	XP_024832120.1	protein SMG7 isoform X11
XM_005217297.3	XP_005217354.1	protein SMG7 isoform X2
XM_015475257.2	XP_015330743.1	protein SMG7 isoform X4
XM_024976349.1	XP_024832117.1	protein SMG7 isoform X8
NM_001193205.2	NP_001180134.2	protein SMG7
XM_005217296.3	XP_005217353.1	protein SMG7 isoform X1
XM_010813526.2	XP_010811828.2	protein SMG7 isoform X3
NM_001193205.3	NP_001180134.2	protein SMG7
XM_024976348.1	XP_024832116.1	protein SMG7 isoform X5
XM_015475258.2	XP_015330744.1	protein SMG7 isoform X6
XM_024976350.1	XP_024832118.1	protein SMG7 isoform X9

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa223660	XM_024976352.1 Latest version!	Bos taurus SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa239010	NM_001193205.3 Latest version!	Bos taurus SMG7 nonsense mediated mRNA decay factor (SMG7), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa223650	XM_005217296.4 Latest version!	Bos taurus SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa223651	XM_005217297.4 Latest version!	Bos taurus SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa223652	XM_010813526.3 Latest version!	Bos taurus SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa223653	XM_015475257.2 Latest version!	Bos taurus SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa223654	XM_024976348.1 Latest version!	Bos taurus SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa223655	XM_015475258.2 Latest version!	Bos taurus SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa223656	XM_010813528.3 Latest version!	Bos taurus SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa223657	XM_024976349.1 Latest version!	Bos taurus SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa223658	XM_024976350.1 Latest version!	Bos taurus SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa223659	XM_024976351.1 Latest version!	Bos taurus SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa93045	XM_010813526.2 Latest version!	Bos taurus SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBa93046	XM_015475257.1 Latest version!	Bos taurus SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBa93047	XM_015475258.1 Latest version!	Bos taurus SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OBa93048	XM_010813528.2 Latest version!	Bos taurus SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OBa39141	NM_001193205.2 Latest version!	Bos taurus SMG7, nonsense mediated mRNA decay factor (SMG7), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa27463	XM_005217296.3 Latest version!	Bos taurus SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa27464	XM_005217297.3 Latest version!	Bos taurus SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

SMG7 ( XM_024976352.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832120.1 Bos taurus SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OBa223660
Clone ID Related Accession (Same CDS sequence)	XM_024976352.1
Accession Version	XM_024976352.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2421bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein SMG7 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTTTC TTGGCATCCT GTGCAAGTGT CCTCTCCAGA ACAAGTCTCA GGAGGAATCC 
TACAATGCCT ATCCCCTACC TGCCGTCAAG GTGTCCATGG ACTGGCTGAG ACTCAGACCC 
AGGGTGTTTC AGGAGGCAGT GGTGGATGAA AGACAGTACA TTTGGCCGTG GCTAATTTCT 
CTTCTGAACA GTTTCCATCC CCATGAAGAA GATCTTTCAA GTACTAATGC AACACCACTT 
CCAGAGGAGT TTGAGTTACA AGGATTCTTG GCTTTGAGAC CTTCTTTCAG GAACTTGGAT 
TTTTCCAAAG GTCACCAGGG TATTACAGGA GACAAAGAGG GTCAGCAACG ACAAATACGA 
CAGCAGCGTT TGATCTCTAT AGGCAAATGG ATTGCAGATA ATCAGCCAAG GCTGATTCAG 
TGTGAAAATG AGGTAGGGAA ATTGTTGTTT ATCACAGAAA TCCCAGAGTT AATACTGGAA 
GACCCCAGTG AAGCCAAAGA GAACCTCATT CTGCAAGACA CAACCATCAT AGAGTCCCTG 
GCAGCTGACG GGAACCCAGG ACTGAAATCA GTGCTATCTA CAGGCCGGAA TCTAAGCAAC 
AACTGCGACC CAGGAGAGAA ACCAGTGGTC ACCTTCAAAG AGAACATGAA GCCACGAGAA 
GTGAGCAGAG ACCAAGGAAG AAGTTTTCCT CCCAAAGAGG TAAAATCTCA GACAGAGTTA 
AGAAAGACTC CAGTGTCTGA AGCCAGGAAA ACACCTGTAA CTCAAACCCC AAGTCAAGCA 
AGTAACTCCC AGTTCATCCC CATTCATCAC CCTGGAGCCT TCCCTCCTCT TCCCAGCCGG 
CCAGGGTTCC CGCCCCCAAC ATATGTTATC CCCCCTCCTG TGGCATTTTC TATGGGCTCA 
GGTTACACCT TCCCAGCTGG TGTTTCTGTC CCAGGAACCT TTCTTCAGCC TACAGCTCAC 
TCTCCACCAG GAAACCAGGT GCAAGCTGGG AAACAGTCCC ACATTCCTTA CAGCCAGCAA 
CGGCCCTCCG GACCAGGGCC AGTGACCCAG GGACCTCAGC AGCCTCAGCC ACCTTCCCAG 
CAGCCCCTCC CACCTCTGCC AGCTCCGCCA GCAGCACAGA CTGCAGGCCA GCTGCAGGTT 
CAAGCTCTAG CTCAGCAGCA ACAGTCCCCT ACAAAAGCTG TGCCGGCTTT GGGGAAAAGT 
CCTCCTCACC ACTCTGGATT CCAGCAGTAT CAACAGGCAG ATGCCTCCAA ACAGCTGTGG 
AATCCCCCTC AGGTTCAAGG CCCATTAGGG AAAATCATGC CTGTGAAACA GCCCTACTAC 
CTTCAAACCC AAGACCCCAT AAAACTGTTT GAGCCGTCGT TGCAACCTCC TGTAAGGCAG 
CAGCAGCCTC TAGAGAAAAC AATGAAGCCT TTCCCCATGG AGCCATATAA CCATAACCCC 
TCAGAAGTCA AGATCCCGGA GTTCTACTGG GATTCTTCCT ACAGCGCTGC TGATAACAGA 
GCTGTGATGG CACAGCAAGC CAGTGTGGAC CGCAGGGGCA AACGGTCCCC GGGAGTCTTC 
CGTCCAGAGC AGGACCCTGT GCCCAGGATG CCATTTGAGG ACCCCAAAGG CTCCCCTCTG 
CTTCCTCCGG ACCTGTTAAA GAGTCTGGCT GCCTTGGAGG AAGAGGAAGA GCTGATTTTT 
TCTAACCCTC CTGATCTTTA CCCAGCTCTG CTGGGGCCTC TCGCCTCTCT TCCTGGACGA 
AGCCTCTTTA AATCCTTACT GGAGAAGCCC TCAGAGCTCA TGTCACATTC ATCCTCTTTC 
CTGTCCCTCA CCGGATTCTC CCTCAATCAG GAGCGATACC CAAATAACAG TGTGTTCAAT 
GAGGTATACG GGAAGAACCT GACAACCAGC TCCAAAGCAG AACTTAATCC CTCAGTGGCT 
CCCCAGGAAA CCTCACTGTA CTCGCTTTTT GAAGGGACTC CATGGTCTCC ATCTCTTCCT 
GCCAGTTCAG ATCATTCAAC ACCAGCCAGC CAATCTCCTC ATTCCTCCAA CCCAAGCAGC 
CTGCCAAGCT CCCCTCCAAC ACACAACCAC AATTCTGTCC CATTCTCCAA TTTTGGACCC 
ATTGGGACTC CAGATAACAG GGATAGGAGA ACTGCAGACC GGTGGAAAAC TGATAAGCCA 
GCCATGGGTG GGTTTGGCAT CGATTATCTC TCAGCAACAT CATCCTCTGA GAGCAGTTGG 
CATCAGGCCA GCACTCCAAG TGGCTCCTGG ACAGGCCATG GCCCATCCAT GGAGGATTCC 
TCTGCTGTCC TCATGGAAAG CCTAAAGTCT ATCTGGTCCA GTTCCATGAT GCATCCTGGA 
CCTTCCGCTT TGGAGCAGCT GTTAATGCAG CAGAAGCAGA AACAGCAGCG GGGCCAAGGC 
GCCATGAACC CTCCACACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832120.1
CDS	1187..3607
Translation	MSFLGILCKCPLQNKSQEESYNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPHEEDLSSTNATPLPEEFELQGFLALRPSFRNLDFSKGHQGITGDKEGQQRQIRQQRLISIGKWIADNQPRLIQCENEVGKLLFITEIPELILEDPSEAKENLILQDTTIIESLAADGNPGLKSVLSTGRNLSNNCDPGEKPVVTFKENMKPREVSRDQGRSFPPKEVKSQTELRKTPVSEARKTPVTQTPSQASNSQFIPIHHPGAFPPLPSRPGFPPPTYVIPPPVAFSMGSGYTFPAGVSVPGTFLQPTAHSPPGNQVQAGKQSHIPYSQQRPSGPGPVTQGPQQPQPPSQQPLPPLPAPPAAQTAGQLQVQALAQQQQSPTKAVPALGKSPPHHSGFQQYQQADASKQLWNPPQVQGPLGKIMPVKQPYYLQTQDPIKLFEPSLQPPVRQQQPLEKTMKPFPMEPYNHNPSEVKIPEFYWDSSYSAADNRAVMAQQASVDRRGKRSPGVFRPEQDPVPRMPFEDPKGSPLLPPDLLKSLAALEEEEELIFSNPPDLYPALLGPLASLPGRSLFKSLLEKPSELMSHSSSFLSLTGFSLNQERYPNNSVFNEVYGKNLTTSSKAELNPSVAPQETSLYSLFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSVPFSNFGPIGTPDNRDRRTADRWKTDKPAMGGFGIDYLSATSSSESSWHQASTPSGSWTGHGPSMEDSSAVLMESLKSIWSSSMMHPGPSALEQLLMQQKQKQQRGQGAMNPPH

Target ORF information:

RefSeq Version	XM_024976352.1
Organism	Bos taurus(cattle)
Definition	Bos taurus SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976352.1

ORF Insert Sequence:

ATGTCTTTTC TTGGCATCCT GTGCAAGTGT CCTCTCCAGA ACAAGTCTCA GGAGGAATCC 
TACAATGCCT ATCCCCTACC TGCCGTCAAG GTGTCCATGG ACTGGCTGAG ACTCAGACCC 
AGGGTGTTTC AGGAGGCAGT GGTGGATGAA AGACAGTACA TTTGGCCGTG GCTAATTTCT 
CTTCTGAACA GTTTCCATCC CCATGAAGAA GATCTTTCAA GTACTAATGC AACACCACTT 
CCAGAGGAGT TTGAGTTACA AGGATTCTTG GCTTTGAGAC CTTCTTTCAG GAACTTGGAT 
TTTTCCAAAG GTCACCAGGG TATTACAGGA GACAAAGAGG GTCAGCAACG ACAAATACGA 
CAGCAGCGTT TGATCTCTAT AGGCAAATGG ATTGCAGATA ATCAGCCAAG GCTGATTCAG 
TGTGAAAATG AGGTAGGGAA ATTGTTGTTT ATCACAGAAA TCCCAGAGTT AATACTGGAA 
GACCCCAGTG AAGCCAAAGA GAACCTCATT CTGCAAGACA CAACCATCAT AGAGTCCCTG 
GCAGCTGACG GGAACCCAGG ACTGAAATCA GTGCTATCTA CAGGCCGGAA TCTAAGCAAC 
AACTGCGACC CAGGAGAGAA ACCAGTGGTC ACCTTCAAAG AGAACATGAA GCCACGAGAA 
GTGAGCAGAG ACCAAGGAAG AAGTTTTCCT CCCAAAGAGG TAAAATCTCA GACAGAGTTA 
AGAAAGACTC CAGTGTCTGA AGCCAGGAAA ACACCTGTAA CTCAAACCCC AAGTCAAGCA 
AGTAACTCCC AGTTCATCCC CATTCATCAC CCTGGAGCCT TCCCTCCTCT TCCCAGCCGG 
CCAGGGTTCC CGCCCCCAAC ATATGTTATC CCCCCTCCTG TGGCATTTTC TATGGGCTCA 
GGTTACACCT TCCCAGCTGG TGTTTCTGTC CCAGGAACCT TTCTTCAGCC TACAGCTCAC 
TCTCCACCAG GAAACCAGGT GCAAGCTGGG AAACAGTCCC ACATTCCTTA CAGCCAGCAA 
CGGCCCTCCG GACCAGGGCC AGTGACCCAG GGACCTCAGC AGCCTCAGCC ACCTTCCCAG 
CAGCCCCTCC CACCTCTGCC AGCTCCGCCA GCAGCACAGA CTGCAGGCCA GCTGCAGGTT 
CAAGCTCTAG CTCAGCAGCA ACAGTCCCCT ACAAAAGCTG TGCCGGCTTT GGGGAAAAGT 
CCTCCTCACC ACTCTGGATT CCAGCAGTAT CAACAGGCAG ATGCCTCCAA ACAGCTGTGG 
AATCCCCCTC AGGTTCAAGG CCCATTAGGG AAAATCATGC CTGTGAAACA GCCCTACTAC 
CTTCAAACCC AAGACCCCAT AAAACTGTTT GAGCCGTCGT TGCAACCTCC TGTAAGGCAG 
CAGCAGCCTC TAGAGAAAAC AATGAAGCCT TTCCCCATGG AGCCATATAA CCATAACCCC 
TCAGAAGTCA AGATCCCGGA GTTCTACTGG GATTCTTCCT ACAGCGCTGC TGATAACAGA 
GCTGTGATGG CACAGCAAGC CAGTGTGGAC CGCAGGGGCA AACGGTCCCC GGGAGTCTTC 
CGTCCAGAGC AGGACCCTGT GCCCAGGATG CCATTTGAGG ACCCCAAAGG CTCCCCTCTG 
CTTCCTCCGG ACCTGTTAAA GAGTCTGGCT GCCTTGGAGG AAGAGGAAGA GCTGATTTTT 
TCTAACCCTC CTGATCTTTA CCCAGCTCTG CTGGGGCCTC TCGCCTCTCT TCCTGGACGA 
AGCCTCTTTA AATCCTTACT GGAGAAGCCC TCAGAGCTCA TGTCACATTC ATCCTCTTTC 
CTGTCCCTCA CCGGATTCTC CCTCAATCAG GAGCGATACC CAAATAACAG TGTGTTCAAT 
GAGGTATACG GGAAGAACCT GACAACCAGC TCCAAAGCAG AACTTAATCC CTCAGTGGCT 
CCCCAGGAAA CCTCACTGTA CTCGCTTTTT GAAGGGACTC CATGGTCTCC ATCTCTTCCT 
GCCAGTTCAG ATCATTCAAC ACCAGCCAGC CAATCTCCTC ATTCCTCCAA CCCAAGCAGC 
CTGCCAAGCT CCCCTCCAAC ACACAACCAC AATTCTGTCC CATTCTCCAA TTTTGGACCC 
ATTGGGACTC CAGATAACAG GGATAGGAGA ACTGCAGACC GGTGGAAAAC TGATAAGCCA 
GCCATGGGTG GGTTTGGCAT CGATTATCTC TCAGCAACAT CATCCTCTGA GAGCAGTTGG 
CATCAGGCCA GCACTCCAAG TGGCTCCTGG ACAGGCCATG GCCCATCCAT GGAGGATTCC 
TCTGCTGTCC TCATGGAAAG CCTAAAGTCT ATCTGGTCCA GTTCCATGAT GCATCCTGGA 
CCTTCCGCTT TGGAGCAGCT GTTAATGCAG CAGAAGCAGA AACAGCAGCG GGGCCAAGGC 
GCCATGAACC CTCCACACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG7 ( NM_001193205.3 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001180134.2 Bos taurus SMG7 nonsense mediated mRNA decay factor (SMG7), mRNA.

Price & Availability↑

CloneID	OBa239010
Clone ID Related Accession (Same CDS sequence)	NM_001193205.3
Accession Version	NM_001193205.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3414bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-29
Organism	Bos taurus(cattle)
Product	protein SMG7
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000016.1. On May 29, 2018 this sequence version replaced NM_001193205.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR6764281.28505.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145414 [ECO:0000348] ##Evidence-Data-END##

ATGAGCCTGC AGAGCGCGCA GTATCTCCGG CAGGCAGAAG TCCTGAAGGC TGACATGACA 
GATTCTAAGC TGGGTCCAGC CGAAGTCTGG ACATCCAGGC AGGCTCTGCA GGACCTGTAC 
CAGAAAATGC TTGTTACTGA TTTGGAATAC GCTTTAGACA AGAAAGTAGA ACAGGATCTC 
TGGAATCATG CCTTTAAGAA TCAGATCACA ACACTACAAG GCCAGGCAAA GAATCGAGCA 
AACCCGAATC GGAGTGAAGT TCAGGCAAAC CTTTCTCTGT TCCTAGAGGC AGCTAGTGGC 
TTCTATACTC AGTTATTACA AGAACTGTGT ACAGTGTTTA ATGTAGATTT ACCATGCCGT 
GTGAAGTCTT CCCAGTTGGG AATTATCAGC AATAAACAGA CGCATACCAG CGCCATAGTG 
AAGCCACAGT CTAGCTCCTG TTCCTATATC TGCCAGCACT GCCTCGTTCA CCTTGGAGAC 
ATTGCTCGAT ACAGAAACCA GACCAGCCAG GCAGAGTCCT ACTATAGGCA TGCGGCTCAG 
CTTGTCCCCT CTAATGGTCA GCCTTACAAT CAGTTGGCTA TCTTAGCTTC TTCCAAAGGA 
GACCATCTGA CTACAATTTT CTACTACTGC AGAAGCATTG CTGTGAAATT CCCTTTCCCA 
GCTGCCTCTA CTAATCTACA AAAAGCACTT TCTAAAGCAC TGGAAAGCCG GGATGAGGTG 
AAAACCAAAT GGGGTGTTTC TGACTTCATC AAGGCCTTTA TTAAATTCCA CGGTCATGTG 
TACCTGAGTA AGAGCTTGGA AAAGTTGAGC CCTCTTCGAG AGAAGTTGGA AGAACAGTTT 
AAGAGGCTGC TATTCCAGAA AGCTTTCAAC TCTCAGCAGT TAGTTCACGT CACTGTCATT 
AACCTGTTTC AACTTCATCA TCTTCGTGAC TTTAGCAACG AAACAGAGCA GCATAGTTAT 
AGCCAAGATG AGCAGCTGTG TTGGACACAG TTGCTGGCCC TCTTTATGTC TTTTCTTGGC 
ATCCTGTGCA AGTGTCCTCT CCAGAACAAG TCTCAGGAGG AATCCTACAA TGCCTATCCC 
CTACCTGCCG TCAAGGTGTC CATGGACTGG CTGAGACTCA GACCCAGGGT GTTTCAGGAG 
GCAGTGGTGG ATGAAAGACA GTACATTTGG CCGTGGCTAA TTTCTCTTCT GAACAGTTTC 
CATCCCCATG AAGAAGATCT TTCAAGTACT AATGCAACAC CACTTCCAGA GGAGTTTGAG 
TTACAAGGAT TCTTGGCTTT GAGACCTTCT TTCAGGAACT TGGATTTTTC CAAAGGTCAC 
CAGGGTATTA CAGGAGACAA AGAGGGTCAG CAACGACAAA TACGACAGCA GCGTTTGATC 
TCTATAGGCA AATGGATTGC AGATAATCAG CCAAGGCTGA TTCAGTGTGA AAATGAGGTA 
GGGAAATTGT TGTTTATCAC AGAAATCCCA GAGTTAATAC TGGAAGACCC CAGTGAAGCC 
AAAGAGAACC TCATTCTGCA AGACACAACC ATCATAGAGT CCCTGGCAGC TGACGGGAAC 
CCAGGACTGA AATCAGTGCT ATCTACAGGC CGGAATCTAA GCAACAACTG CGACCCAGGA 
GAGAAACCAG TGGTCACCTT CAAAGAGAAC ATGAAGCCAC GAGAAGTGAG CAGAGACCAA 
GGAAGAAGTT TTCCTCCCAA AGAGGTGAGA AGGGACTATA GCAAAGGAGT AGCTGTAACT 
GAGAATGATG GAAAGAAGGA CAACAACAAG AGGAAAACAG AAACCAAGAA ATGCACCTTA 
GAAAAGTTAC AGGAAACAGG AAAGCAGAGT GTGGCAGTGC AGGTAAAATC TCAGACAGAG 
TTAAGAAAGA CTCCAGTGTC TGAAGCCAGG AAAACACCTG TAACTCAAAC CCCAAGTCAA 
GCAAGTAACT CCCAGTTCAT CCCCATTCAT CACCCTGGAG CCTTCCCTCC TCTTCCCAGC 
CGGCCAGGGT TCCCGCCCCC AACATATGTT ATCCCCCCTC CTGTGGCATT TTCTATGGGC 
TCAGGTTACA CCTTCCCAGC TGGTGTTTCT GTCCCAGGAA CCTTTCTTCA GCCTACAGCT 
CACTCTCCAC CAGGAAACCA GGTGCAAGCT GGGAAACAGT CCCACATTCC TTACAGCCAG 
CAACGGCCCT CCGGACCAGG GCCAGTGACC CAGGGACCTC AGCAGCCTCA GCCACCTTCC 
CAGCAGCCCC TCCCACCTCT GCCAGCTCCG CCAGCAGCAC AGACTGCAGG CCAGCTGCAG 
GTTCAAGCTC TAGCTCAGCA GCAACAGTCC CCTACAAAAG CTGTGCCGGC TTTGGGGAAA 
AGTCCTCCTC ACCACTCTGG ATTCCAGCAG TATCAACAGG CAGATGCCTC CAAACAGCTG 
TGGAATCCCC CTCAGGTTCA AGGCCCATTA GGGAAAATCA TGCCTGTGAA ACAGCCCTAC 
TACCTTCAAA CCCAAGACCC CATAAAACTG TTTGAGCCGT CGTTGCAACC TCCTGTAAGG 
CAGCAGCAGC CTCTAGAGAA AACAATGAAG CCTTTCCCCA TGGAGCCATA TAACCATAAC 
CCCTCAGAAG TCAAGATCCC GGAGTTCTAC TGGGATTCTT CCTACAGCGC TGCTGATAAC 
AGAGCTGTGA TGGCACAGCA AGCCAGTGTG GACCGCAGGG GCAAACGGTC CCCGGGAGTC 
TTCCGTCCAG AGCAGGACCC TGTGCCCAGG ATGCCATTTG AGAAATCCTT ACTGGAGAAG 
CCCTCAGAGC TCATGTCACA TTCATCCTCT TTCCTGTCCC TCACCGGATT CTCCCTCAAT 
CAGGAGCGAT ACCCAAATAA CAGTGTGTTC AATGAGGTAT ACGGGAAGAA CCTGACAACC 
AGCTCCAAAG CAGAACTTAA TCCCTCAGTG GCTCCCCAGG AAACCTCACT GTACTCGCTT 
TTTGAAGGGA CTCCATGGTC TCCATCTCTT CCTGCCAGTT CAGATCATTC AACACCAGCC 
AGCCAATCTC CTCATTCCTC CAACCCAAGC AGCCTGCCAA GCTCCCCTCC AACACACAAC 
CACAATTCTG TCCCATTCTC CAATTTTGGA CCCATTGGGA CTCCAGATAA CAGGGATAGG 
AGAACTGCAG ACCGGTGGAA AACTGATAAG CCAGCCATGG GTGGGTTTGG CATCGATTAT 
CTCTCAGCAA CATCATCCTC TGAGAGCAGT TGGCATCAGG CCAGCACTCC AAGTGGCTCC 
TGGACAGGCC ATGGCCCATC CATGGAGGAT TCCTCTGCTG TCCTCATGGA AAGCCTAAAG 
TCTATCTGGT CCAGTTCCAT GATGCATCCT GGACCTTCCG CTTTGGAGCA GCTGTTAATG 
CAGCAGAAGC AGAAACAGCA GCGGGGCCAA GGCGCCATGA ACCCTCCACA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001180134.2
CDS	154..3567
Translation	MSLQSAQYLRQAEVLKADMTDSKLGPAEVWTSRQALQDLYQKMLVTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRANPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQTHTSAIVKPQSSSCSYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNLQKALSKALESRDEVKTKWGVSDFIKAFIKFHGHVYLSKSLEKLSPLREKLEEQFKRLLFQKAFNSQQLVHVTVINLFQLHHLRDFSNETEQHSYSQDEQLCWTQLLALFMSFLGILCKCPLQNKSQEESYNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPHEEDLSSTNATPLPEEFELQGFLALRPSFRNLDFSKGHQGITGDKEGQQRQIRQQRLISIGKWIADNQPRLIQCENEVGKLLFITEIPELILEDPSEAKENLILQDTTIIESLAADGNPGLKSVLSTGRNLSNNCDPGEKPVVTFKENMKPREVSRDQGRSFPPKEVRRDYSKGVAVTENDGKKDNNKRKTETKKCTLEKLQETGKQSVAVQVKSQTELRKTPVSEARKTPVTQTPSQASNSQFIPIHHPGAFPPLPSRPGFPPPTYVIPPPVAFSMGSGYTFPAGVSVPGTFLQPTAHSPPGNQVQAGKQSHIPYSQQRPSGPGPVTQGPQQPQPPSQQPLPPLPAPPAAQTAGQLQVQALAQQQQSPTKAVPALGKSPPHHSGFQQYQQADASKQLWNPPQVQGPLGKIMPVKQPYYLQTQDPIKLFEPSLQPPVRQQQPLEKTMKPFPMEPYNHNPSEVKIPEFYWDSSYSAADNRAVMAQQASVDRRGKRSPGVFRPEQDPVPRMPFEKSLLEKPSELMSHSSSFLSLTGFSLNQERYPNNSVFNEVYGKNLTTSSKAELNPSVAPQETSLYSLFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSVPFSNFGPIGTPDNRDRRTADRWKTDKPAMGGFGIDYLSATSSSESSWHQASTPSGSWTGHGPSMEDSSAVLMESLKSIWSSSMMHPGPSALEQLLMQQKQKQQRGQGAMNPPH

Target ORF information:

RefSeq Version	NM_001193205.3
Organism	Bos taurus(cattle)
Definition	Bos taurus SMG7 nonsense mediated mRNA decay factor (SMG7), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001193205.3

ORF Insert Sequence:

ATGAGCCTGC AGAGCGCGCA GTATCTCCGG CAGGCAGAAG TCCTGAAGGC TGACATGACA 
GATTCTAAGC TGGGTCCAGC CGAAGTCTGG ACATCCAGGC AGGCTCTGCA GGACCTGTAC 
CAGAAAATGC TTGTTACTGA TTTGGAATAC GCTTTAGACA AGAAAGTAGA ACAGGATCTC 
TGGAATCATG CCTTTAAGAA TCAGATCACA ACACTACAAG GCCAGGCAAA GAATCGAGCA 
AACCCGAATC GGAGTGAAGT TCAGGCAAAC CTTTCTCTGT TCCTAGAGGC AGCTAGTGGC 
TTCTATACTC AGTTATTACA AGAACTGTGT ACAGTGTTTA ATGTAGATTT ACCATGCCGT 
GTGAAGTCTT CCCAGTTGGG AATTATCAGC AATAAACAGA CGCATACCAG CGCCATAGTG 
AAGCCACAGT CTAGCTCCTG TTCCTATATC TGCCAGCACT GCCTCGTTCA CCTTGGAGAC 
ATTGCTCGAT ACAGAAACCA GACCAGCCAG GCAGAGTCCT ACTATAGGCA TGCGGCTCAG 
CTTGTCCCCT CTAATGGTCA GCCTTACAAT CAGTTGGCTA TCTTAGCTTC TTCCAAAGGA 
GACCATCTGA CTACAATTTT CTACTACTGC AGAAGCATTG CTGTGAAATT CCCTTTCCCA 
GCTGCCTCTA CTAATCTACA AAAAGCACTT TCTAAAGCAC TGGAAAGCCG GGATGAGGTG 
AAAACCAAAT GGGGTGTTTC TGACTTCATC AAGGCCTTTA TTAAATTCCA CGGTCATGTG 
TACCTGAGTA AGAGCTTGGA AAAGTTGAGC CCTCTTCGAG AGAAGTTGGA AGAACAGTTT 
AAGAGGCTGC TATTCCAGAA AGCTTTCAAC TCTCAGCAGT TAGTTCACGT CACTGTCATT 
AACCTGTTTC AACTTCATCA TCTTCGTGAC TTTAGCAACG AAACAGAGCA GCATAGTTAT 
AGCCAAGATG AGCAGCTGTG TTGGACACAG TTGCTGGCCC TCTTTATGTC TTTTCTTGGC 
ATCCTGTGCA AGTGTCCTCT CCAGAACAAG TCTCAGGAGG AATCCTACAA TGCCTATCCC 
CTACCTGCCG TCAAGGTGTC CATGGACTGG CTGAGACTCA GACCCAGGGT GTTTCAGGAG 
GCAGTGGTGG ATGAAAGACA GTACATTTGG CCGTGGCTAA TTTCTCTTCT GAACAGTTTC 
CATCCCCATG AAGAAGATCT TTCAAGTACT AATGCAACAC CACTTCCAGA GGAGTTTGAG 
TTACAAGGAT TCTTGGCTTT GAGACCTTCT TTCAGGAACT TGGATTTTTC CAAAGGTCAC 
CAGGGTATTA CAGGAGACAA AGAGGGTCAG CAACGACAAA TACGACAGCA GCGTTTGATC 
TCTATAGGCA AATGGATTGC AGATAATCAG CCAAGGCTGA TTCAGTGTGA AAATGAGGTA 
GGGAAATTGT TGTTTATCAC AGAAATCCCA GAGTTAATAC TGGAAGACCC CAGTGAAGCC 
AAAGAGAACC TCATTCTGCA AGACACAACC ATCATAGAGT CCCTGGCAGC TGACGGGAAC 
CCAGGACTGA AATCAGTGCT ATCTACAGGC CGGAATCTAA GCAACAACTG CGACCCAGGA 
GAGAAACCAG TGGTCACCTT CAAAGAGAAC ATGAAGCCAC GAGAAGTGAG CAGAGACCAA 
GGAAGAAGTT TTCCTCCCAA AGAGGTGAGA AGGGACTATA GCAAAGGAGT AGCTGTAACT 
GAGAATGATG GAAAGAAGGA CAACAACAAG AGGAAAACAG AAACCAAGAA ATGCACCTTA 
GAAAAGTTAC AGGAAACAGG AAAGCAGAGT GTGGCAGTGC AGGTAAAATC TCAGACAGAG 
TTAAGAAAGA CTCCAGTGTC TGAAGCCAGG AAAACACCTG TAACTCAAAC CCCAAGTCAA 
GCAAGTAACT CCCAGTTCAT CCCCATTCAT CACCCTGGAG CCTTCCCTCC TCTTCCCAGC 
CGGCCAGGGT TCCCGCCCCC AACATATGTT ATCCCCCCTC CTGTGGCATT TTCTATGGGC 
TCAGGTTACA CCTTCCCAGC TGGTGTTTCT GTCCCAGGAA CCTTTCTTCA GCCTACAGCT 
CACTCTCCAC CAGGAAACCA GGTGCAAGCT GGGAAACAGT CCCACATTCC TTACAGCCAG 
CAACGGCCCT CCGGACCAGG GCCAGTGACC CAGGGACCTC AGCAGCCTCA GCCACCTTCC 
CAGCAGCCCC TCCCACCTCT GCCAGCTCCG CCAGCAGCAC AGACTGCAGG CCAGCTGCAG 
GTTCAAGCTC TAGCTCAGCA GCAACAGTCC CCTACAAAAG CTGTGCCGGC TTTGGGGAAA 
AGTCCTCCTC ACCACTCTGG ATTCCAGCAG TATCAACAGG CAGATGCCTC CAAACAGCTG 
TGGAATCCCC CTCAGGTTCA AGGCCCATTA GGGAAAATCA TGCCTGTGAA ACAGCCCTAC 
TACCTTCAAA CCCAAGACCC CATAAAACTG TTTGAGCCGT CGTTGCAACC TCCTGTAAGG 
CAGCAGCAGC CTCTAGAGAA AACAATGAAG CCTTTCCCCA TGGAGCCATA TAACCATAAC 
CCCTCAGAAG TCAAGATCCC GGAGTTCTAC TGGGATTCTT CCTACAGCGC TGCTGATAAC 
AGAGCTGTGA TGGCACAGCA AGCCAGTGTG GACCGCAGGG GCAAACGGTC CCCGGGAGTC 
TTCCGTCCAG AGCAGGACCC TGTGCCCAGG ATGCCATTTG AGAAATCCTT ACTGGAGAAG 
CCCTCAGAGC TCATGTCACA TTCATCCTCT TTCCTGTCCC TCACCGGATT CTCCCTCAAT 
CAGGAGCGAT ACCCAAATAA CAGTGTGTTC AATGAGGTAT ACGGGAAGAA CCTGACAACC 
AGCTCCAAAG CAGAACTTAA TCCCTCAGTG GCTCCCCAGG AAACCTCACT GTACTCGCTT 
TTTGAAGGGA CTCCATGGTC TCCATCTCTT CCTGCCAGTT CAGATCATTC AACACCAGCC 
AGCCAATCTC CTCATTCCTC CAACCCAAGC AGCCTGCCAA GCTCCCCTCC AACACACAAC 
CACAATTCTG TCCCATTCTC CAATTTTGGA CCCATTGGGA CTCCAGATAA CAGGGATAGG 
AGAACTGCAG ACCGGTGGAA AACTGATAAG CCAGCCATGG GTGGGTTTGG CATCGATTAT 
CTCTCAGCAA CATCATCCTC TGAGAGCAGT TGGCATCAGG CCAGCACTCC AAGTGGCTCC 
TGGACAGGCC ATGGCCCATC CATGGAGGAT TCCTCTGCTG TCCTCATGGA AAGCCTAAAG 
TCTATCTGGT CCAGTTCCAT GATGCATCCT GGACCTTCCG CTTTGGAGCA GCTGTTAATG 
CAGCAGAAGC AGAAACAGCA GCGGGGCCAA GGCGCCATGA ACCCTCCACA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG7 ( XM_005217296.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005217353.1 Bos taurus SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa223650
Clone ID Related Accession (Same CDS sequence)	XM_005217296.4
Accession Version	XM_005217296.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3651bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein SMG7 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005217296.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
CAGATGAGGA CCGGAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG 
GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCCGA AGTCTGGACA 
TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTTG TTACTGATTT GGAATACGCT 
TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCATGCCT TTAAGAATCA GATCACAACA 
CTACAAGGCC AGGCAAAGAA TCGAGCAAAC CCGAATCGGA GTGAAGTTCA GGCAAACCTT 
TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA 
GTGTTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AGTTGGGAAT TATCAGCAAT 
AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC 
CAGCACTGCC TCGTTCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA 
GAGTCCTACT ATAGGCATGC GGCTCAGCTT GTCCCCTCTA ATGGTCAGCC TTACAATCAG 
TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACTA CAATTTTCTA CTACTGCAGA 
AGCATTGCTG TGAAATTCCC TTTCCCAGCT GCCTCTACTA ATCTACAAAA AGCACTTTCT 
AAAGCACTGG AAAGCCGGGA TGAGGTGAAA ACCAAATGGG GTGTTTCTGA CTTCATCAAG 
GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT 
CTTCGAGAGA AGTTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAGAAAGC TTTCAACTCT 
CAGCAGTTAG TTCACGTCAC TGTCATTAAC CTGTTTCAAC TTCATCATCT TCGTGACTTT 
AGCAACGAAA CAGAGCAGCA TAGTTATAGC CAAGATGAGC AGCTGTGTTG GACACAGTTG 
CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTCCA GAACAAGTCT 
CAGGAGGAAT CCTACAATGC CTATCCCCTA CCTGCCGTCA AGGTGTCCAT GGACTGGCTG 
AGACTCAGAC CCAGGGTGTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCG 
TGGCTAATTT CTCTTCTGAA CAGTTTCCAT CCCCATGAAG AAGATCTTTC AAGTACTAAT 
GCAACACCAC TTCCAGAGGA GTTTGAGTTA CAAGGATTCT TGGCTTTGAG ACCTTCTTTC 
AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GAGACAAAGA GGGTCAGCAA 
CGACAAATAC GACAGCAGCG TTTGATCTCT ATAGGCAAAT GGATTGCAGA TAATCAGCCA 
AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAG 
TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA CACAACCATC 
ATAGAGTCCC TGGCAGCTGA CGGGAACCCA GGACTGAAAT CAGTGCTATC TACAGGCCGG 
AATCTAAGCA ACAACTGCGA CCCAGGAGAG AAACCAGTGG TCACCTTCAA AGAGAACATG 
AAGCCACGAG AAGTGAGCAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTGAGAAGG 
GACTATAGCA AAGGAGTAGC TGTAACTGAG AATGATGGAA AGAAGGACAA CAACAAGAGG 
AAAACAGAAA CCAAGAAATG CACCTTAGAA AAGTTACAGG AAACAGGAAA GCAGAGTGTG 
GCAGTGCAGG TAAAATCTCA GACAGAGTTA AGAAAGACTC CAGTGTCTGA AGCCAGGAAA 
ACACCTGTAA CTCAAACCCC AAGTCAAGCA AGTAACTCCC AGTTCATCCC CATTCATCAC 
CCTGGAGCCT TCCCTCCTCT TCCCAGCCGG CCAGGGTTCC CGCCCCCAAC ATATGTTATC 
CCCCCTCCTG TGGCATTTTC TATGGGCTCA GGTTACACCT TCCCAGCTGG TGTTTCTGTC 
CCAGGAACCT TTCTTCAGCC TACAGCTCAC TCTCCACCAG GAAACCAGGT GCAAGCTGGG 
AAACAGTCCC ACATTCCTTA CAGCCAGCAA CGGCCCTCCG GACCAGGGCC AGTGACCCAG 
GGACCTCAGC AGCCTCAGCC ACCTTCCCAG CAGCCCCTCC CACCTCTGCC AGCTCCGCCA 
GCAGCACAGA CTGCAGGCCA GCTGCAGGTT CAAGCTCTAG CTCAGCAGCA ACAGTCCCCT 
ACAAAAGCTG TGCCGGCTTT GGGGAAAAGT CCTCCTCACC ACTCTGGATT CCAGCAGTAT 
CAACAGGCAG ATGCCTCCAA ACAGCTGTGG AATCCCCCTC AGGTTCAAGG CCCATTAGGG 
AAAATCATGC CTGTGAAACA GCCCTACTAC CTTCAAACCC AAGACCCCAT AAAACTGTTT 
GAGCCGTCGT TGCAACCTCC TGTAAGGCAG CAGCAGCCTC TAGAGAAAAC AATGAAGCCT 
TTCCCCATGG AGCCATATAA CCATAACCCC TCAGAAGTCA AGATCCCGGA GTTCTACTGG 
GATTCTTCCT ACAGCGCTGC TGATAACAGA GCTGTGATGG CACAGCAAGC CAGTGTGGAC 
CGCAGGGGCA AACGGTCCCC GGGAGTCTTC CGTCCAGAGC AGGACCCTGT GCCCAGGATG 
CCATTTGAGG ACCCCAAAGG CTCCCCTCTG CTTCCTCCGG ACCTGTTAAA GAGTCTGGCT 
GCCTTGGAGG AAGAGGAAGA GCTGATTTTT TCTAACCCTC CTGATCTTTA CCCAGCTCTG 
CTGGGGCCTC TCGCCTCTCT TCCTGGACGA AGCCTCTTTA AATCCTTACT GGAGAAGCCC 
TCAGAGCTCA TGTCACATTC ATCCTCTTTC CTGTCCCTCA CCGGATTCTC CCTCAATCAG 
GAGCGATACC CAAATAACAG TGTGTTCAAT GAGGTATACG GGAAGAACCT GACAACCAGC 
TCCAAAGCAG AACTTAATCC CTCAGTGGCT CCCCAGGAAA CCTCACTGTA CTCGCTTTTT 
GAAGGGACTC CATGGTCTCC ATCTCTTCCT GCCAGTTCAG ATCATTCAAC ACCAGCCAGC 
CAATCTCCTC ATTCCTCCAA CCCAAGCAGC CTGCCAAGCT CCCCTCCAAC ACACAACCAC 
AATTCTGTCC CATTCTCCAA TTTTGGACCC ATTGGGACTC CAGATAACAG GGATAGGAGA 
ACTGCAGACC GGTGGAAAAC TGATAAGCCA GCCATGGGTG GGTTTGGCAT CGATTATCTC 
TCAGCAACAT CATCCTCTGA GAGCAGTTGG CATCAGGCCA GCACTCCAAG TGGCTCCTGG 
ACAGGCCATG GCCCATCCAT GGAGGATTCC TCTGCTGTCC TCATGGAAAG CCTAAAGTCT 
ATCTGGTCCA GTTCCATGAT GCATCCTGGA CCTTCCGCTT TGGAGCAGCT GTTAATGCAG 
CAGAAGCAGA AACAGCAGCG GGGCCAAGGC GCCATGAACC CTCCACACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005217353.1
CDS	149..3799
Translation	MRNWMFLQIVIVGENVSFKSQMRTGNLKSEEHLKSSNIRQAEVLKADMTDSKLGPAEVWTSRQALQDLYQKMLVTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRANPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQTHTSAIVKPQSSSCSYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNLQKALSKALESRDEVKTKWGVSDFIKAFIKFHGHVYLSKSLEKLSPLREKLEEQFKRLLFQKAFNSQQLVHVTVINLFQLHHLRDFSNETEQHSYSQDEQLCWTQLLALFMSFLGILCKCPLQNKSQEESYNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPHEEDLSSTNATPLPEEFELQGFLALRPSFRNLDFSKGHQGITGDKEGQQRQIRQQRLISIGKWIADNQPRLIQCENEVGKLLFITEIPELILEDPSEAKENLILQDTTIIESLAADGNPGLKSVLSTGRNLSNNCDPGEKPVVTFKENMKPREVSRDQGRSFPPKEVRRDYSKGVAVTENDGKKDNNKRKTETKKCTLEKLQETGKQSVAVQVKSQTELRKTPVSEARKTPVTQTPSQASNSQFIPIHHPGAFPPLPSRPGFPPPTYVIPPPVAFSMGSGYTFPAGVSVPGTFLQPTAHSPPGNQVQAGKQSHIPYSQQRPSGPGPVTQGPQQPQPPSQQPLPPLPAPPAAQTAGQLQVQALAQQQQSPTKAVPALGKSPPHHSGFQQYQQADASKQLWNPPQVQGPLGKIMPVKQPYYLQTQDPIKLFEPSLQPPVRQQQPLEKTMKPFPMEPYNHNPSEVKIPEFYWDSSYSAADNRAVMAQQASVDRRGKRSPGVFRPEQDPVPRMPFEDPKGSPLLPPDLLKSLAALEEEEELIFSNPPDLYPALLGPLASLPGRSLFKSLLEKPSELMSHSSSFLSLTGFSLNQERYPNNSVFNEVYGKNLTTSSKAELNPSVAPQETSLYSLFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSVPFSNFGPIGTPDNRDRRTADRWKTDKPAMGGFGIDYLSATSSSESSWHQASTPSGSWTGHGPSMEDSSAVLMESLKSIWSSSMMHPGPSALEQLLMQQKQKQQRGQGAMNPPH

Target ORF information:

RefSeq Version	XM_005217296.4
Organism	Bos taurus(cattle)
Definition	Bos taurus SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005217296.4

ORF Insert Sequence:

ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
CAGATGAGGA CCGGAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG 
GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCCGA AGTCTGGACA 
TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTTG TTACTGATTT GGAATACGCT 
TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCATGCCT TTAAGAATCA GATCACAACA 
CTACAAGGCC AGGCAAAGAA TCGAGCAAAC CCGAATCGGA GTGAAGTTCA GGCAAACCTT 
TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA 
GTGTTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AGTTGGGAAT TATCAGCAAT 
AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC 
CAGCACTGCC TCGTTCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA 
GAGTCCTACT ATAGGCATGC GGCTCAGCTT GTCCCCTCTA ATGGTCAGCC TTACAATCAG 
TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACTA CAATTTTCTA CTACTGCAGA 
AGCATTGCTG TGAAATTCCC TTTCCCAGCT GCCTCTACTA ATCTACAAAA AGCACTTTCT 
AAAGCACTGG AAAGCCGGGA TGAGGTGAAA ACCAAATGGG GTGTTTCTGA CTTCATCAAG 
GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT 
CTTCGAGAGA AGTTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAGAAAGC TTTCAACTCT 
CAGCAGTTAG TTCACGTCAC TGTCATTAAC CTGTTTCAAC TTCATCATCT TCGTGACTTT 
AGCAACGAAA CAGAGCAGCA TAGTTATAGC CAAGATGAGC AGCTGTGTTG GACACAGTTG 
CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTCCA GAACAAGTCT 
CAGGAGGAAT CCTACAATGC CTATCCCCTA CCTGCCGTCA AGGTGTCCAT GGACTGGCTG 
AGACTCAGAC CCAGGGTGTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCG 
TGGCTAATTT CTCTTCTGAA CAGTTTCCAT CCCCATGAAG AAGATCTTTC AAGTACTAAT 
GCAACACCAC TTCCAGAGGA GTTTGAGTTA CAAGGATTCT TGGCTTTGAG ACCTTCTTTC 
AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GAGACAAAGA GGGTCAGCAA 
CGACAAATAC GACAGCAGCG TTTGATCTCT ATAGGCAAAT GGATTGCAGA TAATCAGCCA 
AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAG 
TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA CACAACCATC 
ATAGAGTCCC TGGCAGCTGA CGGGAACCCA GGACTGAAAT CAGTGCTATC TACAGGCCGG 
AATCTAAGCA ACAACTGCGA CCCAGGAGAG AAACCAGTGG TCACCTTCAA AGAGAACATG 
AAGCCACGAG AAGTGAGCAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTGAGAAGG 
GACTATAGCA AAGGAGTAGC TGTAACTGAG AATGATGGAA AGAAGGACAA CAACAAGAGG 
AAAACAGAAA CCAAGAAATG CACCTTAGAA AAGTTACAGG AAACAGGAAA GCAGAGTGTG 
GCAGTGCAGG TAAAATCTCA GACAGAGTTA AGAAAGACTC CAGTGTCTGA AGCCAGGAAA 
ACACCTGTAA CTCAAACCCC AAGTCAAGCA AGTAACTCCC AGTTCATCCC CATTCATCAC 
CCTGGAGCCT TCCCTCCTCT TCCCAGCCGG CCAGGGTTCC CGCCCCCAAC ATATGTTATC 
CCCCCTCCTG TGGCATTTTC TATGGGCTCA GGTTACACCT TCCCAGCTGG TGTTTCTGTC 
CCAGGAACCT TTCTTCAGCC TACAGCTCAC TCTCCACCAG GAAACCAGGT GCAAGCTGGG 
AAACAGTCCC ACATTCCTTA CAGCCAGCAA CGGCCCTCCG GACCAGGGCC AGTGACCCAG 
GGACCTCAGC AGCCTCAGCC ACCTTCCCAG CAGCCCCTCC CACCTCTGCC AGCTCCGCCA 
GCAGCACAGA CTGCAGGCCA GCTGCAGGTT CAAGCTCTAG CTCAGCAGCA ACAGTCCCCT 
ACAAAAGCTG TGCCGGCTTT GGGGAAAAGT CCTCCTCACC ACTCTGGATT CCAGCAGTAT 
CAACAGGCAG ATGCCTCCAA ACAGCTGTGG AATCCCCCTC AGGTTCAAGG CCCATTAGGG 
AAAATCATGC CTGTGAAACA GCCCTACTAC CTTCAAACCC AAGACCCCAT AAAACTGTTT 
GAGCCGTCGT TGCAACCTCC TGTAAGGCAG CAGCAGCCTC TAGAGAAAAC AATGAAGCCT 
TTCCCCATGG AGCCATATAA CCATAACCCC TCAGAAGTCA AGATCCCGGA GTTCTACTGG 
GATTCTTCCT ACAGCGCTGC TGATAACAGA GCTGTGATGG CACAGCAAGC CAGTGTGGAC 
CGCAGGGGCA AACGGTCCCC GGGAGTCTTC CGTCCAGAGC AGGACCCTGT GCCCAGGATG 
CCATTTGAGG ACCCCAAAGG CTCCCCTCTG CTTCCTCCGG ACCTGTTAAA GAGTCTGGCT 
GCCTTGGAGG AAGAGGAAGA GCTGATTTTT TCTAACCCTC CTGATCTTTA CCCAGCTCTG 
CTGGGGCCTC TCGCCTCTCT TCCTGGACGA AGCCTCTTTA AATCCTTACT GGAGAAGCCC 
TCAGAGCTCA TGTCACATTC ATCCTCTTTC CTGTCCCTCA CCGGATTCTC CCTCAATCAG 
GAGCGATACC CAAATAACAG TGTGTTCAAT GAGGTATACG GGAAGAACCT GACAACCAGC 
TCCAAAGCAG AACTTAATCC CTCAGTGGCT CCCCAGGAAA CCTCACTGTA CTCGCTTTTT 
GAAGGGACTC CATGGTCTCC ATCTCTTCCT GCCAGTTCAG ATCATTCAAC ACCAGCCAGC 
CAATCTCCTC ATTCCTCCAA CCCAAGCAGC CTGCCAAGCT CCCCTCCAAC ACACAACCAC 
AATTCTGTCC CATTCTCCAA TTTTGGACCC ATTGGGACTC CAGATAACAG GGATAGGAGA 
ACTGCAGACC GGTGGAAAAC TGATAAGCCA GCCATGGGTG GGTTTGGCAT CGATTATCTC 
TCAGCAACAT CATCCTCTGA GAGCAGTTGG CATCAGGCCA GCACTCCAAG TGGCTCCTGG 
ACAGGCCATG GCCCATCCAT GGAGGATTCC TCTGCTGTCC TCATGGAAAG CCTAAAGTCT 
ATCTGGTCCA GTTCCATGAT GCATCCTGGA CCTTCCGCTT TGGAGCAGCT GTTAATGCAG 
CAGAAGCAGA AACAGCAGCG GGGCCAAGGC GCCATGAACC CTCCACACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG7 ( XM_005217297.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005217354.1 Bos taurus SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa223651
Clone ID Related Accession (Same CDS sequence)	XM_005217297.4
Accession Version	XM_005217297.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3564bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein SMG7 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005217297.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCTGC AGAGCGCGCA GTATCTCCGG CAGGCAGAAG TCCTGAAGGC TGACATGACA 
GATTCTAAGC TGGGTCCAGC CGAAGTCTGG ACATCCAGGC AGGCTCTGCA GGACCTGTAC 
CAGAAAATGC TTGTTACTGA TTTGGAATAC GCTTTAGACA AGAAAGTAGA ACAGGATCTC 
TGGAATCATG CCTTTAAGAA TCAGATCACA ACACTACAAG GCCAGGCAAA GAATCGAGCA 
AACCCGAATC GGAGTGAAGT TCAGGCAAAC CTTTCTCTGT TCCTAGAGGC AGCTAGTGGC 
TTCTATACTC AGTTATTACA AGAACTGTGT ACAGTGTTTA ATGTAGATTT ACCATGCCGT 
GTGAAGTCTT CCCAGTTGGG AATTATCAGC AATAAACAGA CGCATACCAG CGCCATAGTG 
AAGCCACAGT CTAGCTCCTG TTCCTATATC TGCCAGCACT GCCTCGTTCA CCTTGGAGAC 
ATTGCTCGAT ACAGAAACCA GACCAGCCAG GCAGAGTCCT ACTATAGGCA TGCGGCTCAG 
CTTGTCCCCT CTAATGGTCA GCCTTACAAT CAGTTGGCTA TCTTAGCTTC TTCCAAAGGA 
GACCATCTGA CTACAATTTT CTACTACTGC AGAAGCATTG CTGTGAAATT CCCTTTCCCA 
GCTGCCTCTA CTAATCTACA AAAAGCACTT TCTAAAGCAC TGGAAAGCCG GGATGAGGTG 
AAAACCAAAT GGGGTGTTTC TGACTTCATC AAGGCCTTTA TTAAATTCCA CGGTCATGTG 
TACCTGAGTA AGAGCTTGGA AAAGTTGAGC CCTCTTCGAG AGAAGTTGGA AGAACAGTTT 
AAGAGGCTGC TATTCCAGAA AGCTTTCAAC TCTCAGCAGT TAGTTCACGT CACTGTCATT 
AACCTGTTTC AACTTCATCA TCTTCGTGAC TTTAGCAACG AAACAGAGCA GCATAGTTAT 
AGCCAAGATG AGCAGCTGTG TTGGACACAG TTGCTGGCCC TCTTTATGTC TTTTCTTGGC 
ATCCTGTGCA AGTGTCCTCT CCAGAACAAG TCTCAGGAGG AATCCTACAA TGCCTATCCC 
CTACCTGCCG TCAAGGTGTC CATGGACTGG CTGAGACTCA GACCCAGGGT GTTTCAGGAG 
GCAGTGGTGG ATGAAAGACA GTACATTTGG CCGTGGCTAA TTTCTCTTCT GAACAGTTTC 
CATCCCCATG AAGAAGATCT TTCAAGTACT AATGCAACAC CACTTCCAGA GGAGTTTGAG 
TTACAAGGAT TCTTGGCTTT GAGACCTTCT TTCAGGAACT TGGATTTTTC CAAAGGTCAC 
CAGGGTATTA CAGGAGACAA AGAGGGTCAG CAACGACAAA TACGACAGCA GCGTTTGATC 
TCTATAGGCA AATGGATTGC AGATAATCAG CCAAGGCTGA TTCAGTGTGA AAATGAGGTA 
GGGAAATTGT TGTTTATCAC AGAAATCCCA GAGTTAATAC TGGAAGACCC CAGTGAAGCC 
AAAGAGAACC TCATTCTGCA AGACACAACC ATCATAGAGT CCCTGGCAGC TGACGGGAAC 
CCAGGACTGA AATCAGTGCT ATCTACAGGC CGGAATCTAA GCAACAACTG CGACCCAGGA 
GAGAAACCAG TGGTCACCTT CAAAGAGAAC ATGAAGCCAC GAGAAGTGAG CAGAGACCAA 
GGAAGAAGTT TTCCTCCCAA AGAGGTGAGA AGGGACTATA GCAAAGGAGT AGCTGTAACT 
GAGAATGATG GAAAGAAGGA CAACAACAAG AGGAAAACAG AAACCAAGAA ATGCACCTTA 
GAAAAGTTAC AGGAAACAGG AAAGCAGAGT GTGGCAGTGC AGGTAAAATC TCAGACAGAG 
TTAAGAAAGA CTCCAGTGTC TGAAGCCAGG AAAACACCTG TAACTCAAAC CCCAAGTCAA 
GCAAGTAACT CCCAGTTCAT CCCCATTCAT CACCCTGGAG CCTTCCCTCC TCTTCCCAGC 
CGGCCAGGGT TCCCGCCCCC AACATATGTT ATCCCCCCTC CTGTGGCATT TTCTATGGGC 
TCAGGTTACA CCTTCCCAGC TGGTGTTTCT GTCCCAGGAA CCTTTCTTCA GCCTACAGCT 
CACTCTCCAC CAGGAAACCA GGTGCAAGCT GGGAAACAGT CCCACATTCC TTACAGCCAG 
CAACGGCCCT CCGGACCAGG GCCAGTGACC CAGGGACCTC AGCAGCCTCA GCCACCTTCC 
CAGCAGCCCC TCCCACCTCT GCCAGCTCCG CCAGCAGCAC AGACTGCAGG CCAGCTGCAG 
GTTCAAGCTC TAGCTCAGCA GCAACAGTCC CCTACAAAAG CTGTGCCGGC TTTGGGGAAA 
AGTCCTCCTC ACCACTCTGG ATTCCAGCAG TATCAACAGG CAGATGCCTC CAAACAGCTG 
TGGAATCCCC CTCAGGTTCA AGGCCCATTA GGGAAAATCA TGCCTGTGAA ACAGCCCTAC 
TACCTTCAAA CCCAAGACCC CATAAAACTG TTTGAGCCGT CGTTGCAACC TCCTGTAAGG 
CAGCAGCAGC CTCTAGAGAA AACAATGAAG CCTTTCCCCA TGGAGCCATA TAACCATAAC 
CCCTCAGAAG TCAAGATCCC GGAGTTCTAC TGGGATTCTT CCTACAGCGC TGCTGATAAC 
AGAGCTGTGA TGGCACAGCA AGCCAGTGTG GACCGCAGGG GCAAACGGTC CCCGGGAGTC 
TTCCGTCCAG AGCAGGACCC TGTGCCCAGG ATGCCATTTG AGGACCCCAA AGGCTCCCCT 
CTGCTTCCTC CGGACCTGTT AAAGAGTCTG GCTGCCTTGG AGGAAGAGGA AGAGCTGATT 
TTTTCTAACC CTCCTGATCT TTACCCAGCT CTGCTGGGGC CTCTCGCCTC TCTTCCTGGA 
CGAAGCCTCT TTAAATCCTT ACTGGAGAAG CCCTCAGAGC TCATGTCACA TTCATCCTCT 
TTCCTGTCCC TCACCGGATT CTCCCTCAAT CAGGAGCGAT ACCCAAATAA CAGTGTGTTC 
AATGAGGTAT ACGGGAAGAA CCTGACAACC AGCTCCAAAG CAGAACTTAA TCCCTCAGTG 
GCTCCCCAGG AAACCTCACT GTACTCGCTT TTTGAAGGGA CTCCATGGTC TCCATCTCTT 
CCTGCCAGTT CAGATCATTC AACACCAGCC AGCCAATCTC CTCATTCCTC CAACCCAAGC 
AGCCTGCCAA GCTCCCCTCC AACACACAAC CACAATTCTG TCCCATTCTC CAATTTTGGA 
CCCATTGGGA CTCCAGATAA CAGGGATAGG AGAACTGCAG ACCGGTGGAA AACTGATAAG 
CCAGCCATGG GTGGGTTTGG CATCGATTAT CTCTCAGCAA CATCATCCTC TGAGAGCAGT 
TGGCATCAGG CCAGCACTCC AAGTGGCTCC TGGACAGGCC ATGGCCCATC CATGGAGGAT 
TCCTCTGCTG TCCTCATGGA AAGCCTAAAG TCTATCTGGT CCAGTTCCAT GATGCATCCT 
GGACCTTCCG CTTTGGAGCA GCTGTTAATG CAGCAGAAGC AGAAACAGCA GCGGGGCCAA 
GGCGCCATGA ACCCTCCACA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005217354.1
CDS	100..3663
Translation	MSLQSAQYLRQAEVLKADMTDSKLGPAEVWTSRQALQDLYQKMLVTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRANPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQTHTSAIVKPQSSSCSYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNLQKALSKALESRDEVKTKWGVSDFIKAFIKFHGHVYLSKSLEKLSPLREKLEEQFKRLLFQKAFNSQQLVHVTVINLFQLHHLRDFSNETEQHSYSQDEQLCWTQLLALFMSFLGILCKCPLQNKSQEESYNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPHEEDLSSTNATPLPEEFELQGFLALRPSFRNLDFSKGHQGITGDKEGQQRQIRQQRLISIGKWIADNQPRLIQCENEVGKLLFITEIPELILEDPSEAKENLILQDTTIIESLAADGNPGLKSVLSTGRNLSNNCDPGEKPVVTFKENMKPREVSRDQGRSFPPKEVRRDYSKGVAVTENDGKKDNNKRKTETKKCTLEKLQETGKQSVAVQVKSQTELRKTPVSEARKTPVTQTPSQASNSQFIPIHHPGAFPPLPSRPGFPPPTYVIPPPVAFSMGSGYTFPAGVSVPGTFLQPTAHSPPGNQVQAGKQSHIPYSQQRPSGPGPVTQGPQQPQPPSQQPLPPLPAPPAAQTAGQLQVQALAQQQQSPTKAVPALGKSPPHHSGFQQYQQADASKQLWNPPQVQGPLGKIMPVKQPYYLQTQDPIKLFEPSLQPPVRQQQPLEKTMKPFPMEPYNHNPSEVKIPEFYWDSSYSAADNRAVMAQQASVDRRGKRSPGVFRPEQDPVPRMPFEDPKGSPLLPPDLLKSLAALEEEEELIFSNPPDLYPALLGPLASLPGRSLFKSLLEKPSELMSHSSSFLSLTGFSLNQERYPNNSVFNEVYGKNLTTSSKAELNPSVAPQETSLYSLFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSVPFSNFGPIGTPDNRDRRTADRWKTDKPAMGGFGIDYLSATSSSESSWHQASTPSGSWTGHGPSMEDSSAVLMESLKSIWSSSMMHPGPSALEQLLMQQKQKQQRGQGAMNPPH

Target ORF information:

RefSeq Version	XM_005217297.4
Organism	Bos taurus(cattle)
Definition	Bos taurus SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005217297.4

ORF Insert Sequence:

ATGAGCCTGC AGAGCGCGCA GTATCTCCGG CAGGCAGAAG TCCTGAAGGC TGACATGACA 
GATTCTAAGC TGGGTCCAGC CGAAGTCTGG ACATCCAGGC AGGCTCTGCA GGACCTGTAC 
CAGAAAATGC TTGTTACTGA TTTGGAATAC GCTTTAGACA AGAAAGTAGA ACAGGATCTC 
TGGAATCATG CCTTTAAGAA TCAGATCACA ACACTACAAG GCCAGGCAAA GAATCGAGCA 
AACCCGAATC GGAGTGAAGT TCAGGCAAAC CTTTCTCTGT TCCTAGAGGC AGCTAGTGGC 
TTCTATACTC AGTTATTACA AGAACTGTGT ACAGTGTTTA ATGTAGATTT ACCATGCCGT 
GTGAAGTCTT CCCAGTTGGG AATTATCAGC AATAAACAGA CGCATACCAG CGCCATAGTG 
AAGCCACAGT CTAGCTCCTG TTCCTATATC TGCCAGCACT GCCTCGTTCA CCTTGGAGAC 
ATTGCTCGAT ACAGAAACCA GACCAGCCAG GCAGAGTCCT ACTATAGGCA TGCGGCTCAG 
CTTGTCCCCT CTAATGGTCA GCCTTACAAT CAGTTGGCTA TCTTAGCTTC TTCCAAAGGA 
GACCATCTGA CTACAATTTT CTACTACTGC AGAAGCATTG CTGTGAAATT CCCTTTCCCA 
GCTGCCTCTA CTAATCTACA AAAAGCACTT TCTAAAGCAC TGGAAAGCCG GGATGAGGTG 
AAAACCAAAT GGGGTGTTTC TGACTTCATC AAGGCCTTTA TTAAATTCCA CGGTCATGTG 
TACCTGAGTA AGAGCTTGGA AAAGTTGAGC CCTCTTCGAG AGAAGTTGGA AGAACAGTTT 
AAGAGGCTGC TATTCCAGAA AGCTTTCAAC TCTCAGCAGT TAGTTCACGT CACTGTCATT 
AACCTGTTTC AACTTCATCA TCTTCGTGAC TTTAGCAACG AAACAGAGCA GCATAGTTAT 
AGCCAAGATG AGCAGCTGTG TTGGACACAG TTGCTGGCCC TCTTTATGTC TTTTCTTGGC 
ATCCTGTGCA AGTGTCCTCT CCAGAACAAG TCTCAGGAGG AATCCTACAA TGCCTATCCC 
CTACCTGCCG TCAAGGTGTC CATGGACTGG CTGAGACTCA GACCCAGGGT GTTTCAGGAG 
GCAGTGGTGG ATGAAAGACA GTACATTTGG CCGTGGCTAA TTTCTCTTCT GAACAGTTTC 
CATCCCCATG AAGAAGATCT TTCAAGTACT AATGCAACAC CACTTCCAGA GGAGTTTGAG 
TTACAAGGAT TCTTGGCTTT GAGACCTTCT TTCAGGAACT TGGATTTTTC CAAAGGTCAC 
CAGGGTATTA CAGGAGACAA AGAGGGTCAG CAACGACAAA TACGACAGCA GCGTTTGATC 
TCTATAGGCA AATGGATTGC AGATAATCAG CCAAGGCTGA TTCAGTGTGA AAATGAGGTA 
GGGAAATTGT TGTTTATCAC AGAAATCCCA GAGTTAATAC TGGAAGACCC CAGTGAAGCC 
AAAGAGAACC TCATTCTGCA AGACACAACC ATCATAGAGT CCCTGGCAGC TGACGGGAAC 
CCAGGACTGA AATCAGTGCT ATCTACAGGC CGGAATCTAA GCAACAACTG CGACCCAGGA 
GAGAAACCAG TGGTCACCTT CAAAGAGAAC ATGAAGCCAC GAGAAGTGAG CAGAGACCAA 
GGAAGAAGTT TTCCTCCCAA AGAGGTGAGA AGGGACTATA GCAAAGGAGT AGCTGTAACT 
GAGAATGATG GAAAGAAGGA CAACAACAAG AGGAAAACAG AAACCAAGAA ATGCACCTTA 
GAAAAGTTAC AGGAAACAGG AAAGCAGAGT GTGGCAGTGC AGGTAAAATC TCAGACAGAG 
TTAAGAAAGA CTCCAGTGTC TGAAGCCAGG AAAACACCTG TAACTCAAAC CCCAAGTCAA 
GCAAGTAACT CCCAGTTCAT CCCCATTCAT CACCCTGGAG CCTTCCCTCC TCTTCCCAGC 
CGGCCAGGGT TCCCGCCCCC AACATATGTT ATCCCCCCTC CTGTGGCATT TTCTATGGGC 
TCAGGTTACA CCTTCCCAGC TGGTGTTTCT GTCCCAGGAA CCTTTCTTCA GCCTACAGCT 
CACTCTCCAC CAGGAAACCA GGTGCAAGCT GGGAAACAGT CCCACATTCC TTACAGCCAG 
CAACGGCCCT CCGGACCAGG GCCAGTGACC CAGGGACCTC AGCAGCCTCA GCCACCTTCC 
CAGCAGCCCC TCCCACCTCT GCCAGCTCCG CCAGCAGCAC AGACTGCAGG CCAGCTGCAG 
GTTCAAGCTC TAGCTCAGCA GCAACAGTCC CCTACAAAAG CTGTGCCGGC TTTGGGGAAA 
AGTCCTCCTC ACCACTCTGG ATTCCAGCAG TATCAACAGG CAGATGCCTC CAAACAGCTG 
TGGAATCCCC CTCAGGTTCA AGGCCCATTA GGGAAAATCA TGCCTGTGAA ACAGCCCTAC 
TACCTTCAAA CCCAAGACCC CATAAAACTG TTTGAGCCGT CGTTGCAACC TCCTGTAAGG 
CAGCAGCAGC CTCTAGAGAA AACAATGAAG CCTTTCCCCA TGGAGCCATA TAACCATAAC 
CCCTCAGAAG TCAAGATCCC GGAGTTCTAC TGGGATTCTT CCTACAGCGC TGCTGATAAC 
AGAGCTGTGA TGGCACAGCA AGCCAGTGTG GACCGCAGGG GCAAACGGTC CCCGGGAGTC 
TTCCGTCCAG AGCAGGACCC TGTGCCCAGG ATGCCATTTG AGGACCCCAA AGGCTCCCCT 
CTGCTTCCTC CGGACCTGTT AAAGAGTCTG GCTGCCTTGG AGGAAGAGGA AGAGCTGATT 
TTTTCTAACC CTCCTGATCT TTACCCAGCT CTGCTGGGGC CTCTCGCCTC TCTTCCTGGA 
CGAAGCCTCT TTAAATCCTT ACTGGAGAAG CCCTCAGAGC TCATGTCACA TTCATCCTCT 
TTCCTGTCCC TCACCGGATT CTCCCTCAAT CAGGAGCGAT ACCCAAATAA CAGTGTGTTC 
AATGAGGTAT ACGGGAAGAA CCTGACAACC AGCTCCAAAG CAGAACTTAA TCCCTCAGTG 
GCTCCCCAGG AAACCTCACT GTACTCGCTT TTTGAAGGGA CTCCATGGTC TCCATCTCTT 
CCTGCCAGTT CAGATCATTC AACACCAGCC AGCCAATCTC CTCATTCCTC CAACCCAAGC 
AGCCTGCCAA GCTCCCCTCC AACACACAAC CACAATTCTG TCCCATTCTC CAATTTTGGA 
CCCATTGGGA CTCCAGATAA CAGGGATAGG AGAACTGCAG ACCGGTGGAA AACTGATAAG 
CCAGCCATGG GTGGGTTTGG CATCGATTAT CTCTCAGCAA CATCATCCTC TGAGAGCAGT 
TGGCATCAGG CCAGCACTCC AAGTGGCTCC TGGACAGGCC ATGGCCCATC CATGGAGGAT 
TCCTCTGCTG TCCTCATGGA AAGCCTAAAG TCTATCTGGT CCAGTTCCAT GATGCATCCT 
GGACCTTCCG CTTTGGAGCA GCTGTTAATG CAGCAGAAGC AGAAACAGCA GCGGGGCCAA 
GGCGCCATGA ACCCTCCACA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG7 ( XM_010813526.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010811828.2 Bos taurus SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X3, mRNA.

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CloneID	OBa223652
Clone ID Related Accession (Same CDS sequence)	XM_010813526.3
Accession Version	XM_010813526.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3513bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein SMG7 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010813526.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
CAGATGAGGA CCGGAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG 
GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCCGA AGTCTGGACA 
TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTTG TTACTGATTT GGAATACGCT 
TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCATGCCT TTAAGAATCA GATCACAACA 
CTACAAGGCC AGGCAAAGAA TCGAGCAAAC CCGAATCGGA GTGAAGTTCA GGCAAACCTT 
TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA 
GTGTTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AGTTGGGAAT TATCAGCAAT 
AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC 
CAGCACTGCC TCGTTCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA 
GAGTCCTACT ATAGGCATGC GGCTCAGCTT GTCCCCTCTA ATGGTCAGCC TTACAATCAG 
TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACTA CAATTTTCTA CTACTGCAGA 
AGCATTGCTG TGAAATTCCC TTTCCCAGCT GCCTCTACTA ATCTACAAAA AGCACTTTCT 
AAAGCACTGG AAAGCCGGGA TGAGGTGAAA ACCAAATGGG GTGTTTCTGA CTTCATCAAG 
GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT 
CTTCGAGAGA AGTTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAGAAAGC TTTCAACTCT 
CAGCAGTTAG TTCACGTCAC TGTCATTAAC CTGTTTCAAC TTCATCATCT TCGTGACTTT 
AGCAACGAAA CAGAGCAGCA TAGTTATAGC CAAGATGAGC AGCTGTGTTG GACACAGTTG 
CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTCCA GAACAAGTCT 
CAGGAGGAAT CCTACAATGC CTATCCCCTA CCTGCCGTCA AGGTGTCCAT GGACTGGCTG 
AGACTCAGAC CCAGGGTGTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCG 
TGGCTAATTT CTCTTCTGAA CAGTTTCCAT CCCCATGAAG AAGATCTTTC AAGTACTAAT 
GCAACACCAC TTCCAGAGGA GTTTGAGTTA CAAGGATTCT TGGCTTTGAG ACCTTCTTTC 
AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GAGACAAAGA GGGTCAGCAA 
CGACAAATAC GACAGCAGCG TTTGATCTCT ATAGGCAAAT GGATTGCAGA TAATCAGCCA 
AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAG 
TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA CACAACCATC 
ATAGAGTCCC TGGCAGCTGA CGGGAACCCA GGACTGAAAT CAGTGCTATC TACAGGCCGG 
AATCTAAGCA ACAACTGCGA CCCAGGAGAG AAACCAGTGG TCACCTTCAA AGAGAACATG 
AAGCCACGAG AAGTGAGCAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTAAAATCT 
CAGACAGAGT TAAGAAAGAC TCCAGTGTCT GAAGCCAGGA AAACACCTGT AACTCAAACC 
CCAAGTCAAG CAAGTAACTC CCAGTTCATC CCCATTCATC ACCCTGGAGC CTTCCCTCCT 
CTTCCCAGCC GGCCAGGGTT CCCGCCCCCA ACATATGTTA TCCCCCCTCC TGTGGCATTT 
TCTATGGGCT CAGGTTACAC CTTCCCAGCT GGTGTTTCTG TCCCAGGAAC CTTTCTTCAG 
CCTACAGCTC ACTCTCCACC AGGAAACCAG GTGCAAGCTG GGAAACAGTC CCACATTCCT 
TACAGCCAGC AACGGCCCTC CGGACCAGGG CCAGTGACCC AGGGACCTCA GCAGCCTCAG 
CCACCTTCCC AGCAGCCCCT CCCACCTCTG CCAGCTCCGC CAGCAGCACA GACTGCAGGC 
CAGCTGCAGG TTCAAGCTCT AGCTCAGCAG CAACAGTCCC CTACAAAAGC TGTGCCGGCT 
TTGGGGAAAA GTCCTCCTCA CCACTCTGGA TTCCAGCAGT ATCAACAGGC AGATGCCTCC 
AAACAGCTGT GGAATCCCCC TCAGGTTCAA GGCCCATTAG GGAAAATCAT GCCTGTGAAA 
CAGCCCTACT ACCTTCAAAC CCAAGACCCC ATAAAACTGT TTGAGCCGTC GTTGCAACCT 
CCTGTAAGGC AGCAGCAGCC TCTAGAGAAA ACAATGAAGC CTTTCCCCAT GGAGCCATAT 
AACCATAACC CCTCAGAAGT CAAGATCCCG GAGTTCTACT GGGATTCTTC CTACAGCGCT 
GCTGATAACA GAGCTGTGAT GGCACAGCAA GCCAGTGTGG ACCGCAGGGG CAAACGGTCC 
CCGGGAGTCT TCCGTCCAGA GCAGGACCCT GTGCCCAGGA TGCCATTTGA GGACCCCAAA 
GGCTCCCCTC TGCTTCCTCC GGACCTGTTA AAGAGTCTGG CTGCCTTGGA GGAAGAGGAA 
GAGCTGATTT TTTCTAACCC TCCTGATCTT TACCCAGCTC TGCTGGGGCC TCTCGCCTCT 
CTTCCTGGAC GAAGCCTCTT TAAATCCTTA CTGGAGAAGC CCTCAGAGCT CATGTCACAT 
TCATCCTCTT TCCTGTCCCT CACCGGATTC TCCCTCAATC AGGAGCGATA CCCAAATAAC 
AGTGTGTTCA ATGAGGTATA CGGGAAGAAC CTGACAACCA GCTCCAAAGC AGAACTTAAT 
CCCTCAGTGG CTCCCCAGGA AACCTCACTG TACTCGCTTT TTGAAGGGAC TCCATGGTCT 
CCATCTCTTC CTGCCAGTTC AGATCATTCA ACACCAGCCA GCCAATCTCC TCATTCCTCC 
AACCCAAGCA GCCTGCCAAG CTCCCCTCCA ACACACAACC ACAATTCTGT CCCATTCTCC 
AATTTTGGAC CCATTGGGAC TCCAGATAAC AGGGATAGGA GAACTGCAGA CCGGTGGAAA 
ACTGATAAGC CAGCCATGGG TGGGTTTGGC ATCGATTATC TCTCAGCAAC ATCATCCTCT 
GAGAGCAGTT GGCATCAGGC CAGCACTCCA AGTGGCTCCT GGACAGGCCA TGGCCCATCC 
ATGGAGGATT CCTCTGCTGT CCTCATGGAA AGCCTAAAGT CTATCTGGTC CAGTTCCATG 
ATGCATCCTG GACCTTCCGC TTTGGAGCAG CTGTTAATGC AGCAGAAGCA GAAACAGCAG 
CGGGGCCAAG GCGCCATGAA CCCTCCACAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010811828.2
CDS	149..3661
Translation	MRNWMFLQIVIVGENVSFKSQMRTGNLKSEEHLKSSNIRQAEVLKADMTDSKLGPAEVWTSRQALQDLYQKMLVTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRANPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQTHTSAIVKPQSSSCSYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNLQKALSKALESRDEVKTKWGVSDFIKAFIKFHGHVYLSKSLEKLSPLREKLEEQFKRLLFQKAFNSQQLVHVTVINLFQLHHLRDFSNETEQHSYSQDEQLCWTQLLALFMSFLGILCKCPLQNKSQEESYNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPHEEDLSSTNATPLPEEFELQGFLALRPSFRNLDFSKGHQGITGDKEGQQRQIRQQRLISIGKWIADNQPRLIQCENEVGKLLFITEIPELILEDPSEAKENLILQDTTIIESLAADGNPGLKSVLSTGRNLSNNCDPGEKPVVTFKENMKPREVSRDQGRSFPPKEVKSQTELRKTPVSEARKTPVTQTPSQASNSQFIPIHHPGAFPPLPSRPGFPPPTYVIPPPVAFSMGSGYTFPAGVSVPGTFLQPTAHSPPGNQVQAGKQSHIPYSQQRPSGPGPVTQGPQQPQPPSQQPLPPLPAPPAAQTAGQLQVQALAQQQQSPTKAVPALGKSPPHHSGFQQYQQADASKQLWNPPQVQGPLGKIMPVKQPYYLQTQDPIKLFEPSLQPPVRQQQPLEKTMKPFPMEPYNHNPSEVKIPEFYWDSSYSAADNRAVMAQQASVDRRGKRSPGVFRPEQDPVPRMPFEDPKGSPLLPPDLLKSLAALEEEEELIFSNPPDLYPALLGPLASLPGRSLFKSLLEKPSELMSHSSSFLSLTGFSLNQERYPNNSVFNEVYGKNLTTSSKAELNPSVAPQETSLYSLFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSVPFSNFGPIGTPDNRDRRTADRWKTDKPAMGGFGIDYLSATSSSESSWHQASTPSGSWTGHGPSMEDSSAVLMESLKSIWSSSMMHPGPSALEQLLMQQKQKQQRGQGAMNPPH

Target ORF information:

RefSeq Version	XM_010813526.3
Organism	Bos taurus(cattle)
Definition	Bos taurus SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010813526.3

ORF Insert Sequence:

ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
CAGATGAGGA CCGGAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG 
GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCCGA AGTCTGGACA 
TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTTG TTACTGATTT GGAATACGCT 
TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCATGCCT TTAAGAATCA GATCACAACA 
CTACAAGGCC AGGCAAAGAA TCGAGCAAAC CCGAATCGGA GTGAAGTTCA GGCAAACCTT 
TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA 
GTGTTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AGTTGGGAAT TATCAGCAAT 
AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC 
CAGCACTGCC TCGTTCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA 
GAGTCCTACT ATAGGCATGC GGCTCAGCTT GTCCCCTCTA ATGGTCAGCC TTACAATCAG 
TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACTA CAATTTTCTA CTACTGCAGA 
AGCATTGCTG TGAAATTCCC TTTCCCAGCT GCCTCTACTA ATCTACAAAA AGCACTTTCT 
AAAGCACTGG AAAGCCGGGA TGAGGTGAAA ACCAAATGGG GTGTTTCTGA CTTCATCAAG 
GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT 
CTTCGAGAGA AGTTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAGAAAGC TTTCAACTCT 
CAGCAGTTAG TTCACGTCAC TGTCATTAAC CTGTTTCAAC TTCATCATCT TCGTGACTTT 
AGCAACGAAA CAGAGCAGCA TAGTTATAGC CAAGATGAGC AGCTGTGTTG GACACAGTTG 
CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTCCA GAACAAGTCT 
CAGGAGGAAT CCTACAATGC CTATCCCCTA CCTGCCGTCA AGGTGTCCAT GGACTGGCTG 
AGACTCAGAC CCAGGGTGTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCG 
TGGCTAATTT CTCTTCTGAA CAGTTTCCAT CCCCATGAAG AAGATCTTTC AAGTACTAAT 
GCAACACCAC TTCCAGAGGA GTTTGAGTTA CAAGGATTCT TGGCTTTGAG ACCTTCTTTC 
AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GAGACAAAGA GGGTCAGCAA 
CGACAAATAC GACAGCAGCG TTTGATCTCT ATAGGCAAAT GGATTGCAGA TAATCAGCCA 
AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAG 
TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA CACAACCATC 
ATAGAGTCCC TGGCAGCTGA CGGGAACCCA GGACTGAAAT CAGTGCTATC TACAGGCCGG 
AATCTAAGCA ACAACTGCGA CCCAGGAGAG AAACCAGTGG TCACCTTCAA AGAGAACATG 
AAGCCACGAG AAGTGAGCAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTAAAATCT 
CAGACAGAGT TAAGAAAGAC TCCAGTGTCT GAAGCCAGGA AAACACCTGT AACTCAAACC 
CCAAGTCAAG CAAGTAACTC CCAGTTCATC CCCATTCATC ACCCTGGAGC CTTCCCTCCT 
CTTCCCAGCC GGCCAGGGTT CCCGCCCCCA ACATATGTTA TCCCCCCTCC TGTGGCATTT 
TCTATGGGCT CAGGTTACAC CTTCCCAGCT GGTGTTTCTG TCCCAGGAAC CTTTCTTCAG 
CCTACAGCTC ACTCTCCACC AGGAAACCAG GTGCAAGCTG GGAAACAGTC CCACATTCCT 
TACAGCCAGC AACGGCCCTC CGGACCAGGG CCAGTGACCC AGGGACCTCA GCAGCCTCAG 
CCACCTTCCC AGCAGCCCCT CCCACCTCTG CCAGCTCCGC CAGCAGCACA GACTGCAGGC 
CAGCTGCAGG TTCAAGCTCT AGCTCAGCAG CAACAGTCCC CTACAAAAGC TGTGCCGGCT 
TTGGGGAAAA GTCCTCCTCA CCACTCTGGA TTCCAGCAGT ATCAACAGGC AGATGCCTCC 
AAACAGCTGT GGAATCCCCC TCAGGTTCAA GGCCCATTAG GGAAAATCAT GCCTGTGAAA 
CAGCCCTACT ACCTTCAAAC CCAAGACCCC ATAAAACTGT TTGAGCCGTC GTTGCAACCT 
CCTGTAAGGC AGCAGCAGCC TCTAGAGAAA ACAATGAAGC CTTTCCCCAT GGAGCCATAT 
AACCATAACC CCTCAGAAGT CAAGATCCCG GAGTTCTACT GGGATTCTTC CTACAGCGCT 
GCTGATAACA GAGCTGTGAT GGCACAGCAA GCCAGTGTGG ACCGCAGGGG CAAACGGTCC 
CCGGGAGTCT TCCGTCCAGA GCAGGACCCT GTGCCCAGGA TGCCATTTGA GGACCCCAAA 
GGCTCCCCTC TGCTTCCTCC GGACCTGTTA AAGAGTCTGG CTGCCTTGGA GGAAGAGGAA 
GAGCTGATTT TTTCTAACCC TCCTGATCTT TACCCAGCTC TGCTGGGGCC TCTCGCCTCT 
CTTCCTGGAC GAAGCCTCTT TAAATCCTTA CTGGAGAAGC CCTCAGAGCT CATGTCACAT 
TCATCCTCTT TCCTGTCCCT CACCGGATTC TCCCTCAATC AGGAGCGATA CCCAAATAAC 
AGTGTGTTCA ATGAGGTATA CGGGAAGAAC CTGACAACCA GCTCCAAAGC AGAACTTAAT 
CCCTCAGTGG CTCCCCAGGA AACCTCACTG TACTCGCTTT TTGAAGGGAC TCCATGGTCT 
CCATCTCTTC CTGCCAGTTC AGATCATTCA ACACCAGCCA GCCAATCTCC TCATTCCTCC 
AACCCAAGCA GCCTGCCAAG CTCCCCTCCA ACACACAACC ACAATTCTGT CCCATTCTCC 
AATTTTGGAC CCATTGGGAC TCCAGATAAC AGGGATAGGA GAACTGCAGA CCGGTGGAAA 
ACTGATAAGC CAGCCATGGG TGGGTTTGGC ATCGATTATC TCTCAGCAAC ATCATCCTCT 
GAGAGCAGTT GGCATCAGGC CAGCACTCCA AGTGGCTCCT GGACAGGCCA TGGCCCATCC 
ATGGAGGATT CCTCTGCTGT CCTCATGGAA AGCCTAAAGT CTATCTGGTC CAGTTCCATG 
ATGCATCCTG GACCTTCCGC TTTGGAGCAG CTGTTAATGC AGCAGAAGCA GAAACAGCAG 
CGGGGCCAAG GCGCCATGAA CCCTCCACAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG7 ( XM_015475257.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015330743.1 Bos taurus SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X4, mRNA.

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CloneID	OBa223653
Clone ID Related Accession (Same CDS sequence)	XM_015475257.2
Accession Version	XM_015475257.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3501bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein SMG7 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015475257.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
CAGATGAGGA CCGGAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG 
GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCCGA AGTCTGGACA 
TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTTG TTACTGATTT GGAATACGCT 
TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCATGCCT TTAAGAATCA GATCACAACA 
CTACAAGGCC AGGCAAAGAA TCGAGCAAAC CCGAATCGGA GTGAAGTTCA GGCAAACCTT 
TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA 
GTGTTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AGTTGGGAAT TATCAGCAAT 
AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC 
CAGCACTGCC TCGTTCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA 
GAGTCCTACT ATAGGCATGC GGCTCAGCTT GTCCCCTCTA ATGGTCAGCC TTACAATCAG 
TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACTA CAATTTTCTA CTACTGCAGA 
AGCATTGCTG TGAAATTCCC TTTCCCAGCT GCCTCTACTA ATCTACAAAA AGCACTTTCT 
AAAGCACTGG AAAGCCGGGA TGAGGTGAAA ACCAAATGGG GTGTTTCTGA CTTCATCAAG 
GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT 
CTTCGAGAGA AGTTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAGAAAGC TTTCAACTCT 
CAGCAGTTAG TTCACGTCAC TGTCATTAAC CTGTTTCAAC TTCATCATCT TCGTGACTTT 
AGCAACGAAA CAGAGCAGCA TAGTTATAGC CAAGATGAGC AGCTGTGTTG GACACAGTTG 
CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTCCA GAACAAGTCT 
CAGGAGGAAT CCTACAATGC CTATCCCCTA CCTGCCGTCA AGGTGTCCAT GGACTGGCTG 
AGACTCAGAC CCAGGGTGTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCG 
TGGCTAATTT CTCTTCTGAA CAGTTTCCAT CCCCATGAAG AAGATCTTTC AAGTACTAAT 
GCAACACCAC TTCCAGAGGA GTTTGAGTTA CAAGGATTCT TGGCTTTGAG ACCTTCTTTC 
AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GAGACAAAGA GGGTCAGCAA 
CGACAAATAC GACAGCAGCG TTTGATCTCT ATAGGCAAAT GGATTGCAGA TAATCAGCCA 
AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAG 
TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA CACAACCATC 
ATAGAGTCCC TGGCAGCTGA CGGGAACCCA GGACTGAAAT CAGTGCTATC TACAGGCCGG 
AATCTAAGCA ACAACTGCGA CCCAGGAGAG AAACCAGTGG TCACCTTCAA AGAGAACATG 
AAGCCACGAG AAGTGAGCAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTGAGAAGG 
GACTATAGCA AAGGAGTAGC TGTAACTGAG AATGATGGAA AGAAGGACAA CAACAAGAGG 
AAAACAGAAA CCAAGAAATG CACCTTAGAA AAGTTACAGG AAACAGGAAA GCAGAGTGTG 
GCAGTGCAGG TAAAATCTCA GACAGAGTTA AGAAAGACTC CAGTGTCTGA AGCCAGGAAA 
ACACCTGTAA CTCAAACCCC AAGTCAAGCA AGTAACTCCC AGTTCATCCC CATTCATCAC 
CCTGGAGCCT TCCCTCCTCT TCCCAGCCGG CCAGGGTTCC CGCCCCCAAC ATATGTTATC 
CCCCCTCCTG TGGCATTTTC TATGGGCTCA GGTTACACCT TCCCAGCTGG TGTTTCTGTC 
CCAGGAACCT TTCTTCAGCC TACAGCTCAC TCTCCACCAG GAAACCAGGT GCAAGCTGGG 
AAACAGTCCC ACATTCCTTA CAGCCAGCAA CGGCCCTCCG GACCAGGGCC AGTGACCCAG 
GGACCTCAGC AGCCTCAGCC ACCTTCCCAG CAGCCCCTCC CACCTCTGCC AGCTCCGCCA 
GCAGCACAGA CTGCAGGCCA GCTGCAGGTT CAAGCTCTAG CTCAGCAGCA ACAGTCCCCT 
ACAAAAGCTG TGCCGGCTTT GGGGAAAAGT CCTCCTCACC ACTCTGGATT CCAGCAGTAT 
CAACAGGCAG ATGCCTCCAA ACAGCTGTGG AATCCCCCTC AGGTTCAAGG CCCATTAGGG 
AAAATCATGC CTGTGAAACA GCCCTACTAC CTTCAAACCC AAGACCCCAT AAAACTGTTT 
GAGCCGTCGT TGCAACCTCC TGTAAGGCAG CAGCAGCCTC TAGAGAAAAC AATGAAGCCT 
TTCCCCATGG AGCCATATAA CCATAACCCC TCAGAAGTCA AGATCCCGGA GTTCTACTGG 
GATTCTTCCT ACAGCGCTGC TGATAACAGA GCTGTGATGG CACAGCAAGC CAGTGTGGAC 
CGCAGGGGCA AACGGTCCCC GGGAGTCTTC CGTCCAGAGC AGGACCCTGT GCCCAGGATG 
CCATTTGAGA AATCCTTACT GGAGAAGCCC TCAGAGCTCA TGTCACATTC ATCCTCTTTC 
CTGTCCCTCA CCGGATTCTC CCTCAATCAG GAGCGATACC CAAATAACAG TGTGTTCAAT 
GAGGTATACG GGAAGAACCT GACAACCAGC TCCAAAGCAG AACTTAATCC CTCAGTGGCT 
CCCCAGGAAA CCTCACTGTA CTCGCTTTTT GAAGGGACTC CATGGTCTCC ATCTCTTCCT 
GCCAGTTCAG ATCATTCAAC ACCAGCCAGC CAATCTCCTC ATTCCTCCAA CCCAAGCAGC 
CTGCCAAGCT CCCCTCCAAC ACACAACCAC AATTCTGTCC CATTCTCCAA TTTTGGACCC 
ATTGGGACTC CAGATAACAG GGATAGGAGA ACTGCAGACC GGTGGAAAAC TGATAAGCCA 
GCCATGGGTG GGTTTGGCAT CGATTATCTC TCAGCAACAT CATCCTCTGA GAGCAGTTGG 
CATCAGGCCA GCACTCCAAG TGGCTCCTGG ACAGGCCATG GCCCATCCAT GGAGGATTCC 
TCTGCTGTCC TCATGGAAAG CCTAAAGTCT ATCTGGTCCA GTTCCATGAT GCATCCTGGA 
CCTTCCGCTT TGGAGCAGCT GTTAATGCAG CAGAAGCAGA AACAGCAGCG GGGCCAAGGC 
GCCATGAACC CTCCACACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015330743.1
CDS	149..3649
Translation	MRNWMFLQIVIVGENVSFKSQMRTGNLKSEEHLKSSNIRQAEVLKADMTDSKLGPAEVWTSRQALQDLYQKMLVTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRANPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQTHTSAIVKPQSSSCSYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNLQKALSKALESRDEVKTKWGVSDFIKAFIKFHGHVYLSKSLEKLSPLREKLEEQFKRLLFQKAFNSQQLVHVTVINLFQLHHLRDFSNETEQHSYSQDEQLCWTQLLALFMSFLGILCKCPLQNKSQEESYNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPHEEDLSSTNATPLPEEFELQGFLALRPSFRNLDFSKGHQGITGDKEGQQRQIRQQRLISIGKWIADNQPRLIQCENEVGKLLFITEIPELILEDPSEAKENLILQDTTIIESLAADGNPGLKSVLSTGRNLSNNCDPGEKPVVTFKENMKPREVSRDQGRSFPPKEVRRDYSKGVAVTENDGKKDNNKRKTETKKCTLEKLQETGKQSVAVQVKSQTELRKTPVSEARKTPVTQTPSQASNSQFIPIHHPGAFPPLPSRPGFPPPTYVIPPPVAFSMGSGYTFPAGVSVPGTFLQPTAHSPPGNQVQAGKQSHIPYSQQRPSGPGPVTQGPQQPQPPSQQPLPPLPAPPAAQTAGQLQVQALAQQQQSPTKAVPALGKSPPHHSGFQQYQQADASKQLWNPPQVQGPLGKIMPVKQPYYLQTQDPIKLFEPSLQPPVRQQQPLEKTMKPFPMEPYNHNPSEVKIPEFYWDSSYSAADNRAVMAQQASVDRRGKRSPGVFRPEQDPVPRMPFEKSLLEKPSELMSHSSSFLSLTGFSLNQERYPNNSVFNEVYGKNLTTSSKAELNPSVAPQETSLYSLFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSVPFSNFGPIGTPDNRDRRTADRWKTDKPAMGGFGIDYLSATSSSESSWHQASTPSGSWTGHGPSMEDSSAVLMESLKSIWSSSMMHPGPSALEQLLMQQKQKQQRGQGAMNPPH

Target ORF information:

RefSeq Version	XM_015475257.2
Organism	Bos taurus(cattle)
Definition	Bos taurus SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015475257.2

ORF Insert Sequence:

ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
CAGATGAGGA CCGGAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG 
GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCCGA AGTCTGGACA 
TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTTG TTACTGATTT GGAATACGCT 
TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCATGCCT TTAAGAATCA GATCACAACA 
CTACAAGGCC AGGCAAAGAA TCGAGCAAAC CCGAATCGGA GTGAAGTTCA GGCAAACCTT 
TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA 
GTGTTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AGTTGGGAAT TATCAGCAAT 
AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC 
CAGCACTGCC TCGTTCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA 
GAGTCCTACT ATAGGCATGC GGCTCAGCTT GTCCCCTCTA ATGGTCAGCC TTACAATCAG 
TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACTA CAATTTTCTA CTACTGCAGA 
AGCATTGCTG TGAAATTCCC TTTCCCAGCT GCCTCTACTA ATCTACAAAA AGCACTTTCT 
AAAGCACTGG AAAGCCGGGA TGAGGTGAAA ACCAAATGGG GTGTTTCTGA CTTCATCAAG 
GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT 
CTTCGAGAGA AGTTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAGAAAGC TTTCAACTCT 
CAGCAGTTAG TTCACGTCAC TGTCATTAAC CTGTTTCAAC TTCATCATCT TCGTGACTTT 
AGCAACGAAA CAGAGCAGCA TAGTTATAGC CAAGATGAGC AGCTGTGTTG GACACAGTTG 
CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTCCA GAACAAGTCT 
CAGGAGGAAT CCTACAATGC CTATCCCCTA CCTGCCGTCA AGGTGTCCAT GGACTGGCTG 
AGACTCAGAC CCAGGGTGTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCG 
TGGCTAATTT CTCTTCTGAA CAGTTTCCAT CCCCATGAAG AAGATCTTTC AAGTACTAAT 
GCAACACCAC TTCCAGAGGA GTTTGAGTTA CAAGGATTCT TGGCTTTGAG ACCTTCTTTC 
AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GAGACAAAGA GGGTCAGCAA 
CGACAAATAC GACAGCAGCG TTTGATCTCT ATAGGCAAAT GGATTGCAGA TAATCAGCCA 
AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAG 
TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA CACAACCATC 
ATAGAGTCCC TGGCAGCTGA CGGGAACCCA GGACTGAAAT CAGTGCTATC TACAGGCCGG 
AATCTAAGCA ACAACTGCGA CCCAGGAGAG AAACCAGTGG TCACCTTCAA AGAGAACATG 
AAGCCACGAG AAGTGAGCAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTGAGAAGG 
GACTATAGCA AAGGAGTAGC TGTAACTGAG AATGATGGAA AGAAGGACAA CAACAAGAGG 
AAAACAGAAA CCAAGAAATG CACCTTAGAA AAGTTACAGG AAACAGGAAA GCAGAGTGTG 
GCAGTGCAGG TAAAATCTCA GACAGAGTTA AGAAAGACTC CAGTGTCTGA AGCCAGGAAA 
ACACCTGTAA CTCAAACCCC AAGTCAAGCA AGTAACTCCC AGTTCATCCC CATTCATCAC 
CCTGGAGCCT TCCCTCCTCT TCCCAGCCGG CCAGGGTTCC CGCCCCCAAC ATATGTTATC 
CCCCCTCCTG TGGCATTTTC TATGGGCTCA GGTTACACCT TCCCAGCTGG TGTTTCTGTC 
CCAGGAACCT TTCTTCAGCC TACAGCTCAC TCTCCACCAG GAAACCAGGT GCAAGCTGGG 
AAACAGTCCC ACATTCCTTA CAGCCAGCAA CGGCCCTCCG GACCAGGGCC AGTGACCCAG 
GGACCTCAGC AGCCTCAGCC ACCTTCCCAG CAGCCCCTCC CACCTCTGCC AGCTCCGCCA 
GCAGCACAGA CTGCAGGCCA GCTGCAGGTT CAAGCTCTAG CTCAGCAGCA ACAGTCCCCT 
ACAAAAGCTG TGCCGGCTTT GGGGAAAAGT CCTCCTCACC ACTCTGGATT CCAGCAGTAT 
CAACAGGCAG ATGCCTCCAA ACAGCTGTGG AATCCCCCTC AGGTTCAAGG CCCATTAGGG 
AAAATCATGC CTGTGAAACA GCCCTACTAC CTTCAAACCC AAGACCCCAT AAAACTGTTT 
GAGCCGTCGT TGCAACCTCC TGTAAGGCAG CAGCAGCCTC TAGAGAAAAC AATGAAGCCT 
TTCCCCATGG AGCCATATAA CCATAACCCC TCAGAAGTCA AGATCCCGGA GTTCTACTGG 
GATTCTTCCT ACAGCGCTGC TGATAACAGA GCTGTGATGG CACAGCAAGC CAGTGTGGAC 
CGCAGGGGCA AACGGTCCCC GGGAGTCTTC CGTCCAGAGC AGGACCCTGT GCCCAGGATG 
CCATTTGAGA AATCCTTACT GGAGAAGCCC TCAGAGCTCA TGTCACATTC ATCCTCTTTC 
CTGTCCCTCA CCGGATTCTC CCTCAATCAG GAGCGATACC CAAATAACAG TGTGTTCAAT 
GAGGTATACG GGAAGAACCT GACAACCAGC TCCAAAGCAG AACTTAATCC CTCAGTGGCT 
CCCCAGGAAA CCTCACTGTA CTCGCTTTTT GAAGGGACTC CATGGTCTCC ATCTCTTCCT 
GCCAGTTCAG ATCATTCAAC ACCAGCCAGC CAATCTCCTC ATTCCTCCAA CCCAAGCAGC 
CTGCCAAGCT CCCCTCCAAC ACACAACCAC AATTCTGTCC CATTCTCCAA TTTTGGACCC 
ATTGGGACTC CAGATAACAG GGATAGGAGA ACTGCAGACC GGTGGAAAAC TGATAAGCCA 
GCCATGGGTG GGTTTGGCAT CGATTATCTC TCAGCAACAT CATCCTCTGA GAGCAGTTGG 
CATCAGGCCA GCACTCCAAG TGGCTCCTGG ACAGGCCATG GCCCATCCAT GGAGGATTCC 
TCTGCTGTCC TCATGGAAAG CCTAAAGTCT ATCTGGTCCA GTTCCATGAT GCATCCTGGA 
CCTTCCGCTT TGGAGCAGCT GTTAATGCAG CAGAAGCAGA AACAGCAGCG GGGCCAAGGC 
GCCATGAACC CTCCACACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG7 ( XM_024976348.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832116.1 Bos taurus SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa223654
Clone ID Related Accession (Same CDS sequence)	XM_024976348.1
Accession Version	XM_024976348.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3426bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein SMG7 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCTGC AGAGCGCGCA GTATCTCCGG CAGGCAGAAG TCCTGAAGGC TGACATGACA 
GATTCTAAGC TGGGTCCAGC CGAAGTCTGG ACATCCAGGC AGGCTCTGCA GGACCTGTAC 
CAGAAAATGC TTGTTACTGA TTTGGAATAC GCTTTAGACA AGAAAGTAGA ACAGGATCTC 
TGGAATCATG CCTTTAAGAA TCAGATCACA ACACTACAAG GCCAGGCAAA GAATCGAGCA 
AACCCGAATC GGAGTGAAGT TCAGGCAAAC CTTTCTCTGT TCCTAGAGGC AGCTAGTGGC 
TTCTATACTC AGTTATTACA AGAACTGTGT ACAGTGTTTA ATGTAGATTT ACCATGCCGT 
GTGAAGTCTT CCCAGTTGGG AATTATCAGC AATAAACAGA CGCATACCAG CGCCATAGTG 
AAGCCACAGT CTAGCTCCTG TTCCTATATC TGCCAGCACT GCCTCGTTCA CCTTGGAGAC 
ATTGCTCGAT ACAGAAACCA GACCAGCCAG GCAGAGTCCT ACTATAGGCA TGCGGCTCAG 
CTTGTCCCCT CTAATGGTCA GCCTTACAAT CAGTTGGCTA TCTTAGCTTC TTCCAAAGGA 
GACCATCTGA CTACAATTTT CTACTACTGC AGAAGCATTG CTGTGAAATT CCCTTTCCCA 
GCTGCCTCTA CTAATCTACA AAAAGCACTT TCTAAAGCAC TGGAAAGCCG GGATGAGGTG 
AAAACCAAAT GGGGTGTTTC TGACTTCATC AAGGCCTTTA TTAAATTCCA CGGTCATGTG 
TACCTGAGTA AGAGCTTGGA AAAGTTGAGC CCTCTTCGAG AGAAGTTGGA AGAACAGTTT 
AAGAGGCTGC TATTCCAGAA AGCTTTCAAC TCTCAGCAGT TAGTTCACGT CACTGTCATT 
AACCTGTTTC AACTTCATCA TCTTCGTGAC TTTAGCAACG AAACAGAGCA GCATAGTTAT 
AGCCAAGATG AGCAGCTGTG TTGGACACAG TTGCTGGCCC TCTTTATGTC TTTTCTTGGC 
ATCCTGTGCA AGTGTCCTCT CCAGAACAAG TCTCAGGAGG AATCCTACAA TGCCTATCCC 
CTACCTGCCG TCAAGGTGTC CATGGACTGG CTGAGACTCA GACCCAGGGT GTTTCAGGAG 
GCAGTGGTGG ATGAAAGACA GTACATTTGG CCGTGGCTAA TTTCTCTTCT GAACAGTTTC 
CATCCCCATG AAGAAGATCT TTCAAGTACT AATGCAACAC CACTTCCAGA GGAGTTTGAG 
TTACAAGGAT TCTTGGCTTT GAGACCTTCT TTCAGGAACT TGGATTTTTC CAAAGGTCAC 
CAGGGTATTA CAGGAGACAA AGAGGGTCAG CAACGACAAA TACGACAGCA GCGTTTGATC 
TCTATAGGCA AATGGATTGC AGATAATCAG CCAAGGCTGA TTCAGTGTGA AAATGAGGTA 
GGGAAATTGT TGTTTATCAC AGAAATCCCA GAGTTAATAC TGGAAGACCC CAGTGAAGCC 
AAAGAGAACC TCATTCTGCA AGACACAACC ATCATAGAGT CCCTGGCAGC TGACGGGAAC 
CCAGGACTGA AATCAGTGCT ATCTACAGGC CGGAATCTAA GCAACAACTG CGACCCAGGA 
GAGAAACCAG TGGTCACCTT CAAAGAGAAC ATGAAGCCAC GAGAAGTGAG CAGAGACCAA 
GGAAGAAGTT TTCCTCCCAA AGAGGTAAAA TCTCAGACAG AGTTAAGAAA GACTCCAGTG 
TCTGAAGCCA GGAAAACACC TGTAACTCAA ACCCCAAGTC AAGCAAGTAA CTCCCAGTTC 
ATCCCCATTC ATCACCCTGG AGCCTTCCCT CCTCTTCCCA GCCGGCCAGG GTTCCCGCCC 
CCAACATATG TTATCCCCCC TCCTGTGGCA TTTTCTATGG GCTCAGGTTA CACCTTCCCA 
GCTGGTGTTT CTGTCCCAGG AACCTTTCTT CAGCCTACAG CTCACTCTCC ACCAGGAAAC 
CAGGTGCAAG CTGGGAAACA GTCCCACATT CCTTACAGCC AGCAACGGCC CTCCGGACCA 
GGGCCAGTGA CCCAGGGACC TCAGCAGCCT CAGCCACCTT CCCAGCAGCC CCTCCCACCT 
CTGCCAGCTC CGCCAGCAGC ACAGACTGCA GGCCAGCTGC AGGTTCAAGC TCTAGCTCAG 
CAGCAACAGT CCCCTACAAA AGCTGTGCCG GCTTTGGGGA AAAGTCCTCC TCACCACTCT 
GGATTCCAGC AGTATCAACA GGCAGATGCC TCCAAACAGC TGTGGAATCC CCCTCAGGTT 
CAAGGCCCAT TAGGGAAAAT CATGCCTGTG AAACAGCCCT ACTACCTTCA AACCCAAGAC 
CCCATAAAAC TGTTTGAGCC GTCGTTGCAA CCTCCTGTAA GGCAGCAGCA GCCTCTAGAG 
AAAACAATGA AGCCTTTCCC CATGGAGCCA TATAACCATA ACCCCTCAGA AGTCAAGATC 
CCGGAGTTCT ACTGGGATTC TTCCTACAGC GCTGCTGATA ACAGAGCTGT GATGGCACAG 
CAAGCCAGTG TGGACCGCAG GGGCAAACGG TCCCCGGGAG TCTTCCGTCC AGAGCAGGAC 
CCTGTGCCCA GGATGCCATT TGAGGACCCC AAAGGCTCCC CTCTGCTTCC TCCGGACCTG 
TTAAAGAGTC TGGCTGCCTT GGAGGAAGAG GAAGAGCTGA TTTTTTCTAA CCCTCCTGAT 
CTTTACCCAG CTCTGCTGGG GCCTCTCGCC TCTCTTCCTG GACGAAGCCT CTTTAAATCC 
TTACTGGAGA AGCCCTCAGA GCTCATGTCA CATTCATCCT CTTTCCTGTC CCTCACCGGA 
TTCTCCCTCA ATCAGGAGCG ATACCCAAAT AACAGTGTGT TCAATGAGGT ATACGGGAAG 
AACCTGACAA CCAGCTCCAA AGCAGAACTT AATCCCTCAG TGGCTCCCCA GGAAACCTCA 
CTGTACTCGC TTTTTGAAGG GACTCCATGG TCTCCATCTC TTCCTGCCAG TTCAGATCAT 
TCAACACCAG CCAGCCAATC TCCTCATTCC TCCAACCCAA GCAGCCTGCC AAGCTCCCCT 
CCAACACACA ACCACAATTC TGTCCCATTC TCCAATTTTG GACCCATTGG GACTCCAGAT 
AACAGGGATA GGAGAACTGC AGACCGGTGG AAAACTGATA AGCCAGCCAT GGGTGGGTTT 
GGCATCGATT ATCTCTCAGC AACATCATCC TCTGAGAGCA GTTGGCATCA GGCCAGCACT 
CCAAGTGGCT CCTGGACAGG CCATGGCCCA TCCATGGAGG ATTCCTCTGC TGTCCTCATG 
GAAAGCCTAA AGTCTATCTG GTCCAGTTCC ATGATGCATC CTGGACCTTC CGCTTTGGAG 
CAGCTGTTAA TGCAGCAGAA GCAGAAACAG CAGCGGGGCC AAGGCGCCAT GAACCCTCCA 
CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832116.1
CDS	248..3673
Translation	MSLQSAQYLRQAEVLKADMTDSKLGPAEVWTSRQALQDLYQKMLVTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRANPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQTHTSAIVKPQSSSCSYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNLQKALSKALESRDEVKTKWGVSDFIKAFIKFHGHVYLSKSLEKLSPLREKLEEQFKRLLFQKAFNSQQLVHVTVINLFQLHHLRDFSNETEQHSYSQDEQLCWTQLLALFMSFLGILCKCPLQNKSQEESYNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPHEEDLSSTNATPLPEEFELQGFLALRPSFRNLDFSKGHQGITGDKEGQQRQIRQQRLISIGKWIADNQPRLIQCENEVGKLLFITEIPELILEDPSEAKENLILQDTTIIESLAADGNPGLKSVLSTGRNLSNNCDPGEKPVVTFKENMKPREVSRDQGRSFPPKEVKSQTELRKTPVSEARKTPVTQTPSQASNSQFIPIHHPGAFPPLPSRPGFPPPTYVIPPPVAFSMGSGYTFPAGVSVPGTFLQPTAHSPPGNQVQAGKQSHIPYSQQRPSGPGPVTQGPQQPQPPSQQPLPPLPAPPAAQTAGQLQVQALAQQQQSPTKAVPALGKSPPHHSGFQQYQQADASKQLWNPPQVQGPLGKIMPVKQPYYLQTQDPIKLFEPSLQPPVRQQQPLEKTMKPFPMEPYNHNPSEVKIPEFYWDSSYSAADNRAVMAQQASVDRRGKRSPGVFRPEQDPVPRMPFEDPKGSPLLPPDLLKSLAALEEEEELIFSNPPDLYPALLGPLASLPGRSLFKSLLEKPSELMSHSSSFLSLTGFSLNQERYPNNSVFNEVYGKNLTTSSKAELNPSVAPQETSLYSLFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSVPFSNFGPIGTPDNRDRRTADRWKTDKPAMGGFGIDYLSATSSSESSWHQASTPSGSWTGHGPSMEDSSAVLMESLKSIWSSSMMHPGPSALEQLLMQQKQKQQRGQGAMNPPH

Target ORF information:

RefSeq Version	XM_024976348.1
Organism	Bos taurus(cattle)
Definition	Bos taurus SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976348.1

ORF Insert Sequence:

ATGAGCCTGC AGAGCGCGCA GTATCTCCGG CAGGCAGAAG TCCTGAAGGC TGACATGACA 
GATTCTAAGC TGGGTCCAGC CGAAGTCTGG ACATCCAGGC AGGCTCTGCA GGACCTGTAC 
CAGAAAATGC TTGTTACTGA TTTGGAATAC GCTTTAGACA AGAAAGTAGA ACAGGATCTC 
TGGAATCATG CCTTTAAGAA TCAGATCACA ACACTACAAG GCCAGGCAAA GAATCGAGCA 
AACCCGAATC GGAGTGAAGT TCAGGCAAAC CTTTCTCTGT TCCTAGAGGC AGCTAGTGGC 
TTCTATACTC AGTTATTACA AGAACTGTGT ACAGTGTTTA ATGTAGATTT ACCATGCCGT 
GTGAAGTCTT CCCAGTTGGG AATTATCAGC AATAAACAGA CGCATACCAG CGCCATAGTG 
AAGCCACAGT CTAGCTCCTG TTCCTATATC TGCCAGCACT GCCTCGTTCA CCTTGGAGAC 
ATTGCTCGAT ACAGAAACCA GACCAGCCAG GCAGAGTCCT ACTATAGGCA TGCGGCTCAG 
CTTGTCCCCT CTAATGGTCA GCCTTACAAT CAGTTGGCTA TCTTAGCTTC TTCCAAAGGA 
GACCATCTGA CTACAATTTT CTACTACTGC AGAAGCATTG CTGTGAAATT CCCTTTCCCA 
GCTGCCTCTA CTAATCTACA AAAAGCACTT TCTAAAGCAC TGGAAAGCCG GGATGAGGTG 
AAAACCAAAT GGGGTGTTTC TGACTTCATC AAGGCCTTTA TTAAATTCCA CGGTCATGTG 
TACCTGAGTA AGAGCTTGGA AAAGTTGAGC CCTCTTCGAG AGAAGTTGGA AGAACAGTTT 
AAGAGGCTGC TATTCCAGAA AGCTTTCAAC TCTCAGCAGT TAGTTCACGT CACTGTCATT 
AACCTGTTTC AACTTCATCA TCTTCGTGAC TTTAGCAACG AAACAGAGCA GCATAGTTAT 
AGCCAAGATG AGCAGCTGTG TTGGACACAG TTGCTGGCCC TCTTTATGTC TTTTCTTGGC 
ATCCTGTGCA AGTGTCCTCT CCAGAACAAG TCTCAGGAGG AATCCTACAA TGCCTATCCC 
CTACCTGCCG TCAAGGTGTC CATGGACTGG CTGAGACTCA GACCCAGGGT GTTTCAGGAG 
GCAGTGGTGG ATGAAAGACA GTACATTTGG CCGTGGCTAA TTTCTCTTCT GAACAGTTTC 
CATCCCCATG AAGAAGATCT TTCAAGTACT AATGCAACAC CACTTCCAGA GGAGTTTGAG 
TTACAAGGAT TCTTGGCTTT GAGACCTTCT TTCAGGAACT TGGATTTTTC CAAAGGTCAC 
CAGGGTATTA CAGGAGACAA AGAGGGTCAG CAACGACAAA TACGACAGCA GCGTTTGATC 
TCTATAGGCA AATGGATTGC AGATAATCAG CCAAGGCTGA TTCAGTGTGA AAATGAGGTA 
GGGAAATTGT TGTTTATCAC AGAAATCCCA GAGTTAATAC TGGAAGACCC CAGTGAAGCC 
AAAGAGAACC TCATTCTGCA AGACACAACC ATCATAGAGT CCCTGGCAGC TGACGGGAAC 
CCAGGACTGA AATCAGTGCT ATCTACAGGC CGGAATCTAA GCAACAACTG CGACCCAGGA 
GAGAAACCAG TGGTCACCTT CAAAGAGAAC ATGAAGCCAC GAGAAGTGAG CAGAGACCAA 
GGAAGAAGTT TTCCTCCCAA AGAGGTAAAA TCTCAGACAG AGTTAAGAAA GACTCCAGTG 
TCTGAAGCCA GGAAAACACC TGTAACTCAA ACCCCAAGTC AAGCAAGTAA CTCCCAGTTC 
ATCCCCATTC ATCACCCTGG AGCCTTCCCT CCTCTTCCCA GCCGGCCAGG GTTCCCGCCC 
CCAACATATG TTATCCCCCC TCCTGTGGCA TTTTCTATGG GCTCAGGTTA CACCTTCCCA 
GCTGGTGTTT CTGTCCCAGG AACCTTTCTT CAGCCTACAG CTCACTCTCC ACCAGGAAAC 
CAGGTGCAAG CTGGGAAACA GTCCCACATT CCTTACAGCC AGCAACGGCC CTCCGGACCA 
GGGCCAGTGA CCCAGGGACC TCAGCAGCCT CAGCCACCTT CCCAGCAGCC CCTCCCACCT 
CTGCCAGCTC CGCCAGCAGC ACAGACTGCA GGCCAGCTGC AGGTTCAAGC TCTAGCTCAG 
CAGCAACAGT CCCCTACAAA AGCTGTGCCG GCTTTGGGGA AAAGTCCTCC TCACCACTCT 
GGATTCCAGC AGTATCAACA GGCAGATGCC TCCAAACAGC TGTGGAATCC CCCTCAGGTT 
CAAGGCCCAT TAGGGAAAAT CATGCCTGTG AAACAGCCCT ACTACCTTCA AACCCAAGAC 
CCCATAAAAC TGTTTGAGCC GTCGTTGCAA CCTCCTGTAA GGCAGCAGCA GCCTCTAGAG 
AAAACAATGA AGCCTTTCCC CATGGAGCCA TATAACCATA ACCCCTCAGA AGTCAAGATC 
CCGGAGTTCT ACTGGGATTC TTCCTACAGC GCTGCTGATA ACAGAGCTGT GATGGCACAG 
CAAGCCAGTG TGGACCGCAG GGGCAAACGG TCCCCGGGAG TCTTCCGTCC AGAGCAGGAC 
CCTGTGCCCA GGATGCCATT TGAGGACCCC AAAGGCTCCC CTCTGCTTCC TCCGGACCTG 
TTAAAGAGTC TGGCTGCCTT GGAGGAAGAG GAAGAGCTGA TTTTTTCTAA CCCTCCTGAT 
CTTTACCCAG CTCTGCTGGG GCCTCTCGCC TCTCTTCCTG GACGAAGCCT CTTTAAATCC 
TTACTGGAGA AGCCCTCAGA GCTCATGTCA CATTCATCCT CTTTCCTGTC CCTCACCGGA 
TTCTCCCTCA ATCAGGAGCG ATACCCAAAT AACAGTGTGT TCAATGAGGT ATACGGGAAG 
AACCTGACAA CCAGCTCCAA AGCAGAACTT AATCCCTCAG TGGCTCCCCA GGAAACCTCA 
CTGTACTCGC TTTTTGAAGG GACTCCATGG TCTCCATCTC TTCCTGCCAG TTCAGATCAT 
TCAACACCAG CCAGCCAATC TCCTCATTCC TCCAACCCAA GCAGCCTGCC AAGCTCCCCT 
CCAACACACA ACCACAATTC TGTCCCATTC TCCAATTTTG GACCCATTGG GACTCCAGAT 
AACAGGGATA GGAGAACTGC AGACCGGTGG AAAACTGATA AGCCAGCCAT GGGTGGGTTT 
GGCATCGATT ATCTCTCAGC AACATCATCC TCTGAGAGCA GTTGGCATCA GGCCAGCACT 
CCAAGTGGCT CCTGGACAGG CCATGGCCCA TCCATGGAGG ATTCCTCTGC TGTCCTCATG 
GAAAGCCTAA AGTCTATCTG GTCCAGTTCC ATGATGCATC CTGGACCTTC CGCTTTGGAG 
CAGCTGTTAA TGCAGCAGAA GCAGAAACAG CAGCGGGGCC AAGGCGCCAT GAACCCTCCA 
CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG7 ( XM_015475258.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015330744.1 Bos taurus SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X6, mRNA.

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CloneID	OBa223655
Clone ID Related Accession (Same CDS sequence)	XM_015475258.2
Accession Version	XM_015475258.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3423bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein SMG7 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015475258.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
CAGATGAGGA CCGGAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG 
GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCCGA AGTCTGGACA 
TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTTG TTACTGATTT GGAATACGCT 
TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCATGCCT TTAAGAATCA GATCACAACA 
CTACAAGGCC AGGCAAAGAA TCGAGCAAAC CCGAATCGGA GTGAAGTTCA GGCAAACCTT 
TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA 
GTGTTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AGTTGGGAAT TATCAGCAAT 
AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC 
CAGCACTGCC TCGTTCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA 
GAGTCCTACT ATAGGCATGC GGCTCAGCTT GTCCCCTCTA ATGGTCAGCC TTACAATCAG 
TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACTA CAATTTTCTA CTACTGCAGA 
AGCATTGCTG TGAAATTCCC TTTCCCAGCT GCCTCTACTA ATCTACAAAA AGCACTTTCT 
AAAGCACTGG AAAGCCGGGA TGAGGTGAAA ACCAAATGGG GTGTTTCTGA CTTCATCAAG 
GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT 
CTTCGAGAGA AGTTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAGAAAGC TTTCAACTCT 
CAGCAGTTAG TTCACGTCAC TGTCATTAAC CTGTTTCAAC TTCATCATCT TCGTGACTTT 
AGCAACGAAA CAGAGCAGCA TAGTTATAGC CAAGATGAGC AGCTGTGTTG GACACAGTTG 
CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTCCA GAACAAGTCT 
CAGGAGGAAT CCTACAATGC CTATCCCCTA CCTGCCGTCA AGGTGTCCAT GGACTGGCTG 
AGACTCAGAC CCAGGGTGTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCG 
TGGCTAATTT CTCTTCTGAA CAGTTTCCAT CCCCATGAAG AAGATCTTTC AAGTACTAAT 
GCAACACCAC TTCCAGAGGA GTTTGAGTTA CAAGGATTCT TGGCTTTGAG ACCTTCTTTC 
AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GAGACAAAGA GGGTCAGCAA 
CGACAAATAC GACAGCAGCG TTTGATCTCT ATAGGCAAAT GGATTGCAGA TAATCAGCCA 
AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAG 
TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA CACAACCATC 
ATAGAGTCCC TGGCAGCTGA CGGGAACCCA GGACTGAAAT CAGTGCTATC TACAGGCCGG 
AATCTAAGCA ACAACTGCGA CCCAGGAGAG AAACCAGTGG TCACCTTCAA AGAGAACATG 
AAGCCACGAG AAGTGAGCAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTAAAATCT 
CAGACAGAGT TAAGAAAGAC TCCAGTGTCT GAAGCCAGGA AAACACCTGT AACTCAAACC 
CCAAGTCAAG CAAGTAACTC CCAGTTCATC CCCATTCATC ACCCTGGAGC CTTCCCTCCT 
CTTCCCAGCC GGCCAGGAAC CTTTCTTCAG CCTACAGCTC ACTCTCCACC AGGAAACCAG 
GTGCAAGCTG GGAAACAGTC CCACATTCCT TACAGCCAGC AACGGCCCTC CGGACCAGGG 
CCAGTGACCC AGGGACCTCA GCAGCCTCAG CCACCTTCCC AGCAGCCCCT CCCACCTCTG 
CCAGCTCCGC CAGCAGCACA GACTGCAGGC CAGCTGCAGG TTCAAGCTCT AGCTCAGCAG 
CAACAGTCCC CTACAAAAGC TGTGCCGGCT TTGGGGAAAA GTCCTCCTCA CCACTCTGGA 
TTCCAGCAGT ATCAACAGGC AGATGCCTCC AAACAGCTGT GGAATCCCCC TCAGGTTCAA 
GGCCCATTAG GGAAAATCAT GCCTGTGAAA CAGCCCTACT ACCTTCAAAC CCAAGACCCC 
ATAAAACTGT TTGAGCCGTC GTTGCAACCT CCTGTAAGGC AGCAGCAGCC TCTAGAGAAA 
ACAATGAAGC CTTTCCCCAT GGAGCCATAT AACCATAACC CCTCAGAAGT CAAGATCCCG 
GAGTTCTACT GGGATTCTTC CTACAGCGCT GCTGATAACA GAGCTGTGAT GGCACAGCAA 
GCCAGTGTGG ACCGCAGGGG CAAACGGTCC CCGGGAGTCT TCCGTCCAGA GCAGGACCCT 
GTGCCCAGGA TGCCATTTGA GGACCCCAAA GGCTCCCCTC TGCTTCCTCC GGACCTGTTA 
AAGAGTCTGG CTGCCTTGGA GGAAGAGGAA GAGCTGATTT TTTCTAACCC TCCTGATCTT 
TACCCAGCTC TGCTGGGGCC TCTCGCCTCT CTTCCTGGAC GAAGCCTCTT TAAATCCTTA 
CTGGAGAAGC CCTCAGAGCT CATGTCACAT TCATCCTCTT TCCTGTCCCT CACCGGATTC 
TCCCTCAATC AGGAGCGATA CCCAAATAAC AGTGTGTTCA ATGAGGTATA CGGGAAGAAC 
CTGACAACCA GCTCCAAAGC AGAACTTAAT CCCTCAGTGG CTCCCCAGGA AACCTCACTG 
TACTCGCTTT TTGAAGGGAC TCCATGGTCT CCATCTCTTC CTGCCAGTTC AGATCATTCA 
ACACCAGCCA GCCAATCTCC TCATTCCTCC AACCCAAGCA GCCTGCCAAG CTCCCCTCCA 
ACACACAACC ACAATTCTGT CCCATTCTCC AATTTTGGAC CCATTGGGAC TCCAGATAAC 
AGGGATAGGA GAACTGCAGA CCGGTGGAAA ACTGATAAGC CAGCCATGGG TGGGTTTGGC 
ATCGATTATC TCTCAGCAAC ATCATCCTCT GAGAGCAGTT GGCATCAGGC CAGCACTCCA 
AGTGGCTCCT GGACAGGCCA TGGCCCATCC ATGGAGGATT CCTCTGCTGT CCTCATGGAA 
AGCCTAAAGT CTATCTGGTC CAGTTCCATG ATGCATCCTG GACCTTCCGC TTTGGAGCAG 
CTGTTAATGC AGCAGAAGCA GAAACAGCAG CGGGGCCAAG GCGCCATGAA CCCTCCACAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015330744.1
CDS	149..3571
Translation	MRNWMFLQIVIVGENVSFKSQMRTGNLKSEEHLKSSNIRQAEVLKADMTDSKLGPAEVWTSRQALQDLYQKMLVTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRANPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQTHTSAIVKPQSSSCSYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNLQKALSKALESRDEVKTKWGVSDFIKAFIKFHGHVYLSKSLEKLSPLREKLEEQFKRLLFQKAFNSQQLVHVTVINLFQLHHLRDFSNETEQHSYSQDEQLCWTQLLALFMSFLGILCKCPLQNKSQEESYNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPHEEDLSSTNATPLPEEFELQGFLALRPSFRNLDFSKGHQGITGDKEGQQRQIRQQRLISIGKWIADNQPRLIQCENEVGKLLFITEIPELILEDPSEAKENLILQDTTIIESLAADGNPGLKSVLSTGRNLSNNCDPGEKPVVTFKENMKPREVSRDQGRSFPPKEVKSQTELRKTPVSEARKTPVTQTPSQASNSQFIPIHHPGAFPPLPSRPGTFLQPTAHSPPGNQVQAGKQSHIPYSQQRPSGPGPVTQGPQQPQPPSQQPLPPLPAPPAAQTAGQLQVQALAQQQQSPTKAVPALGKSPPHHSGFQQYQQADASKQLWNPPQVQGPLGKIMPVKQPYYLQTQDPIKLFEPSLQPPVRQQQPLEKTMKPFPMEPYNHNPSEVKIPEFYWDSSYSAADNRAVMAQQASVDRRGKRSPGVFRPEQDPVPRMPFEDPKGSPLLPPDLLKSLAALEEEEELIFSNPPDLYPALLGPLASLPGRSLFKSLLEKPSELMSHSSSFLSLTGFSLNQERYPNNSVFNEVYGKNLTTSSKAELNPSVAPQETSLYSLFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSVPFSNFGPIGTPDNRDRRTADRWKTDKPAMGGFGIDYLSATSSSESSWHQASTPSGSWTGHGPSMEDSSAVLMESLKSIWSSSMMHPGPSALEQLLMQQKQKQQRGQGAMNPPH

Target ORF information:

RefSeq Version	XM_015475258.2
Organism	Bos taurus(cattle)
Definition	Bos taurus SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015475258.2

ORF Insert Sequence:

ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
CAGATGAGGA CCGGAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG 
GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCCGA AGTCTGGACA 
TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTTG TTACTGATTT GGAATACGCT 
TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCATGCCT TTAAGAATCA GATCACAACA 
CTACAAGGCC AGGCAAAGAA TCGAGCAAAC CCGAATCGGA GTGAAGTTCA GGCAAACCTT 
TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA 
GTGTTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AGTTGGGAAT TATCAGCAAT 
AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC 
CAGCACTGCC TCGTTCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA 
GAGTCCTACT ATAGGCATGC GGCTCAGCTT GTCCCCTCTA ATGGTCAGCC TTACAATCAG 
TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACTA CAATTTTCTA CTACTGCAGA 
AGCATTGCTG TGAAATTCCC TTTCCCAGCT GCCTCTACTA ATCTACAAAA AGCACTTTCT 
AAAGCACTGG AAAGCCGGGA TGAGGTGAAA ACCAAATGGG GTGTTTCTGA CTTCATCAAG 
GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT 
CTTCGAGAGA AGTTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAGAAAGC TTTCAACTCT 
CAGCAGTTAG TTCACGTCAC TGTCATTAAC CTGTTTCAAC TTCATCATCT TCGTGACTTT 
AGCAACGAAA CAGAGCAGCA TAGTTATAGC CAAGATGAGC AGCTGTGTTG GACACAGTTG 
CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTCCA GAACAAGTCT 
CAGGAGGAAT CCTACAATGC CTATCCCCTA CCTGCCGTCA AGGTGTCCAT GGACTGGCTG 
AGACTCAGAC CCAGGGTGTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCG 
TGGCTAATTT CTCTTCTGAA CAGTTTCCAT CCCCATGAAG AAGATCTTTC AAGTACTAAT 
GCAACACCAC TTCCAGAGGA GTTTGAGTTA CAAGGATTCT TGGCTTTGAG ACCTTCTTTC 
AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GAGACAAAGA GGGTCAGCAA 
CGACAAATAC GACAGCAGCG TTTGATCTCT ATAGGCAAAT GGATTGCAGA TAATCAGCCA 
AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAG 
TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA CACAACCATC 
ATAGAGTCCC TGGCAGCTGA CGGGAACCCA GGACTGAAAT CAGTGCTATC TACAGGCCGG 
AATCTAAGCA ACAACTGCGA CCCAGGAGAG AAACCAGTGG TCACCTTCAA AGAGAACATG 
AAGCCACGAG AAGTGAGCAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTAAAATCT 
CAGACAGAGT TAAGAAAGAC TCCAGTGTCT GAAGCCAGGA AAACACCTGT AACTCAAACC 
CCAAGTCAAG CAAGTAACTC CCAGTTCATC CCCATTCATC ACCCTGGAGC CTTCCCTCCT 
CTTCCCAGCC GGCCAGGAAC CTTTCTTCAG CCTACAGCTC ACTCTCCACC AGGAAACCAG 
GTGCAAGCTG GGAAACAGTC CCACATTCCT TACAGCCAGC AACGGCCCTC CGGACCAGGG 
CCAGTGACCC AGGGACCTCA GCAGCCTCAG CCACCTTCCC AGCAGCCCCT CCCACCTCTG 
CCAGCTCCGC CAGCAGCACA GACTGCAGGC CAGCTGCAGG TTCAAGCTCT AGCTCAGCAG 
CAACAGTCCC CTACAAAAGC TGTGCCGGCT TTGGGGAAAA GTCCTCCTCA CCACTCTGGA 
TTCCAGCAGT ATCAACAGGC AGATGCCTCC AAACAGCTGT GGAATCCCCC TCAGGTTCAA 
GGCCCATTAG GGAAAATCAT GCCTGTGAAA CAGCCCTACT ACCTTCAAAC CCAAGACCCC 
ATAAAACTGT TTGAGCCGTC GTTGCAACCT CCTGTAAGGC AGCAGCAGCC TCTAGAGAAA 
ACAATGAAGC CTTTCCCCAT GGAGCCATAT AACCATAACC CCTCAGAAGT CAAGATCCCG 
GAGTTCTACT GGGATTCTTC CTACAGCGCT GCTGATAACA GAGCTGTGAT GGCACAGCAA 
GCCAGTGTGG ACCGCAGGGG CAAACGGTCC CCGGGAGTCT TCCGTCCAGA GCAGGACCCT 
GTGCCCAGGA TGCCATTTGA GGACCCCAAA GGCTCCCCTC TGCTTCCTCC GGACCTGTTA 
AAGAGTCTGG CTGCCTTGGA GGAAGAGGAA GAGCTGATTT TTTCTAACCC TCCTGATCTT 
TACCCAGCTC TGCTGGGGCC TCTCGCCTCT CTTCCTGGAC GAAGCCTCTT TAAATCCTTA 
CTGGAGAAGC CCTCAGAGCT CATGTCACAT TCATCCTCTT TCCTGTCCCT CACCGGATTC 
TCCCTCAATC AGGAGCGATA CCCAAATAAC AGTGTGTTCA ATGAGGTATA CGGGAAGAAC 
CTGACAACCA GCTCCAAAGC AGAACTTAAT CCCTCAGTGG CTCCCCAGGA AACCTCACTG 
TACTCGCTTT TTGAAGGGAC TCCATGGTCT CCATCTCTTC CTGCCAGTTC AGATCATTCA 
ACACCAGCCA GCCAATCTCC TCATTCCTCC AACCCAAGCA GCCTGCCAAG CTCCCCTCCA 
ACACACAACC ACAATTCTGT CCCATTCTCC AATTTTGGAC CCATTGGGAC TCCAGATAAC 
AGGGATAGGA GAACTGCAGA CCGGTGGAAA ACTGATAAGC CAGCCATGGG TGGGTTTGGC 
ATCGATTATC TCTCAGCAAC ATCATCCTCT GAGAGCAGTT GGCATCAGGC CAGCACTCCA 
AGTGGCTCCT GGACAGGCCA TGGCCCATCC ATGGAGGATT CCTCTGCTGT CCTCATGGAA 
AGCCTAAAGT CTATCTGGTC CAGTTCCATG ATGCATCCTG GACCTTCCGC TTTGGAGCAG 
CTGTTAATGC AGCAGAAGCA GAAACAGCAG CGGGGCCAAG GCGCCATGAA CCCTCCACAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG7 ( XM_010813528.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010811830.2 Bos taurus SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OBa223656
Clone ID Related Accession (Same CDS sequence)	XM_010813528.3
Accession Version	XM_010813528.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3363bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein SMG7 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010813528.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
CAGATGAGGA CCGGAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG 
GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCCGA AGTCTGGACA 
TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTTG TTACTGATTT GGAATACGCT 
TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCATGCCT TTAAGAATCA GATCACAACA 
CTACAAGGCC AGGCAAAGAA TCGAGCAAAC CCGAATCGGA GTGAAGTTCA GGCAAACCTT 
TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA 
GTGTTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AGTTGGGAAT TATCAGCAAT 
AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC 
CAGCACTGCC TCGTTCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA 
GAGTCCTACT ATAGGCATGC GGCTCAGCTT GTCCCCTCTA ATGGTCAGCC TTACAATCAG 
TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACTA CAATTTTCTA CTACTGCAGA 
AGCATTGCTG TGAAATTCCC TTTCCCAGCT GCCTCTACTA ATCTACAAAA AGCACTTTCT 
AAAGCACTGG AAAGCCGGGA TGAGGTGAAA ACCAAATGGG GTGTTTCTGA CTTCATCAAG 
GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT 
CTTCGAGAGA AGTTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAGAAAGC TTTCAACTCT 
CAGCAGTTAG TTCACGTCAC TGTCATTAAC CTGTTTCAAC TTCATCATCT TCGTGACTTT 
AGCAACGAAA CAGAGCAGCA TAGTTATAGC CAAGATGAGC AGCTGTGTTG GACACAGTTG 
CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTCCA GAACAAGTCT 
CAGGAGGAAT CCTACAATGC CTATCCCCTA CCTGCCGTCA AGGTGTCCAT GGACTGGCTG 
AGACTCAGAC CCAGGGTGTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCG 
TGGCTAATTT CTCTTCTGAA CAGTTTCCAT CCCCATGAAG AAGATCTTTC AAGTACTAAT 
GCAACACCAC TTCCAGAGGA GTTTGAGTTA CAAGGATTCT TGGCTTTGAG ACCTTCTTTC 
AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GAGACAAAGA GGGTCAGCAA 
CGACAAATAC GACAGCAGCG TTTGATCTCT ATAGGCAAAT GGATTGCAGA TAATCAGCCA 
AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAG 
TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA CACAACCATC 
ATAGAGTCCC TGGCAGCTGA CGGGAACCCA GGACTGAAAT CAGTGCTATC TACAGGCCGG 
AATCTAAGCA ACAACTGCGA CCCAGGAGAG AAACCAGTGG TCACCTTCAA AGAGAACATG 
AAGCCACGAG AAGTGAGCAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTAAAATCT 
CAGACAGAGT TAAGAAAGAC TCCAGTGTCT GAAGCCAGGA AAACACCTGT AACTCAAACC 
CCAAGTCAAG CAAGTAACTC CCAGTTCATC CCCATTCATC ACCCTGGAGC CTTCCCTCCT 
CTTCCCAGCC GGCCAGGGTT CCCGCCCCCA ACATATGTTA TCCCCCCTCC TGTGGCATTT 
TCTATGGGCT CAGGTTACAC CTTCCCAGCT GGTGTTTCTG TCCCAGGAAC CTTTCTTCAG 
CCTACAGCTC ACTCTCCACC AGGAAACCAG GTGCAAGCTG GGAAACAGTC CCACATTCCT 
TACAGCCAGC AACGGCCCTC CGGACCAGGG CCAGTGACCC AGGGACCTCA GCAGCCTCAG 
CCACCTTCCC AGCAGCCCCT CCCACCTCTG CCAGCTCCGC CAGCAGCACA GACTGCAGGC 
CAGCTGCAGG TTCAAGCTCT AGCTCAGCAG CAACAGTCCC CTACAAAAGC TGTGCCGGCT 
TTGGGGAAAA GTCCTCCTCA CCACTCTGGA TTCCAGCAGT ATCAACAGGC AGATGCCTCC 
AAACAGCTGT GGAATCCCCC TCAGGTTCAA GGCCCATTAG GGAAAATCAT GCCTGTGAAA 
CAGCCCTACT ACCTTCAAAC CCAAGACCCC ATAAAACTGT TTGAGCCGTC GTTGCAACCT 
CCTGTAAGGC AGCAGCAGCC TCTAGAGAAA ACAATGAAGC CTTTCCCCAT GGAGCCATAT 
AACCATAACC CCTCAGAAGT CAAGATCCCG GAGTTCTACT GGGATTCTTC CTACAGCGCT 
GCTGATAACA GAGCTGTGAT GGCACAGCAA GCCAGTGTGG ACCGCAGGGG CAAACGGTCC 
CCGGGAGTCT TCCGTCCAGA GCAGGACCCT GTGCCCAGGA TGCCATTTGA GAAATCCTTA 
CTGGAGAAGC CCTCAGAGCT CATGTCACAT TCATCCTCTT TCCTGTCCCT CACCGGATTC 
TCCCTCAATC AGGAGCGATA CCCAAATAAC AGTGTGTTCA ATGAGGTATA CGGGAAGAAC 
CTGACAACCA GCTCCAAAGC AGAACTTAAT CCCTCAGTGG CTCCCCAGGA AACCTCACTG 
TACTCGCTTT TTGAAGGGAC TCCATGGTCT CCATCTCTTC CTGCCAGTTC AGATCATTCA 
ACACCAGCCA GCCAATCTCC TCATTCCTCC AACCCAAGCA GCCTGCCAAG CTCCCCTCCA 
ACACACAACC ACAATTCTGT CCCATTCTCC AATTTTGGAC CCATTGGGAC TCCAGATAAC 
AGGGATAGGA GAACTGCAGA CCGGTGGAAA ACTGATAAGC CAGCCATGGG TGGGTTTGGC 
ATCGATTATC TCTCAGCAAC ATCATCCTCT GAGAGCAGTT GGCATCAGGC CAGCACTCCA 
AGTGGCTCCT GGACAGGCCA TGGCCCATCC ATGGAGGATT CCTCTGCTGT CCTCATGGAA 
AGCCTAAAGT CTATCTGGTC CAGTTCCATG ATGCATCCTG GACCTTCCGC TTTGGAGCAG 
CTGTTAATGC AGCAGAAGCA GAAACAGCAG CGGGGCCAAG GCGCCATGAA CCCTCCACAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010811830.2
CDS	149..3511
Translation	MRNWMFLQIVIVGENVSFKSQMRTGNLKSEEHLKSSNIRQAEVLKADMTDSKLGPAEVWTSRQALQDLYQKMLVTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRANPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQTHTSAIVKPQSSSCSYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNLQKALSKALESRDEVKTKWGVSDFIKAFIKFHGHVYLSKSLEKLSPLREKLEEQFKRLLFQKAFNSQQLVHVTVINLFQLHHLRDFSNETEQHSYSQDEQLCWTQLLALFMSFLGILCKCPLQNKSQEESYNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPHEEDLSSTNATPLPEEFELQGFLALRPSFRNLDFSKGHQGITGDKEGQQRQIRQQRLISIGKWIADNQPRLIQCENEVGKLLFITEIPELILEDPSEAKENLILQDTTIIESLAADGNPGLKSVLSTGRNLSNNCDPGEKPVVTFKENMKPREVSRDQGRSFPPKEVKSQTELRKTPVSEARKTPVTQTPSQASNSQFIPIHHPGAFPPLPSRPGFPPPTYVIPPPVAFSMGSGYTFPAGVSVPGTFLQPTAHSPPGNQVQAGKQSHIPYSQQRPSGPGPVTQGPQQPQPPSQQPLPPLPAPPAAQTAGQLQVQALAQQQQSPTKAVPALGKSPPHHSGFQQYQQADASKQLWNPPQVQGPLGKIMPVKQPYYLQTQDPIKLFEPSLQPPVRQQQPLEKTMKPFPMEPYNHNPSEVKIPEFYWDSSYSAADNRAVMAQQASVDRRGKRSPGVFRPEQDPVPRMPFEKSLLEKPSELMSHSSSFLSLTGFSLNQERYPNNSVFNEVYGKNLTTSSKAELNPSVAPQETSLYSLFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSVPFSNFGPIGTPDNRDRRTADRWKTDKPAMGGFGIDYLSATSSSESSWHQASTPSGSWTGHGPSMEDSSAVLMESLKSIWSSSMMHPGPSALEQLLMQQKQKQQRGQGAMNPPH

Target ORF information:

RefSeq Version	XM_010813528.3
Organism	Bos taurus(cattle)
Definition	Bos taurus SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010813528.3

ORF Insert Sequence:

ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
CAGATGAGGA CCGGAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG 
GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCCGA AGTCTGGACA 
TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTTG TTACTGATTT GGAATACGCT 
TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCATGCCT TTAAGAATCA GATCACAACA 
CTACAAGGCC AGGCAAAGAA TCGAGCAAAC CCGAATCGGA GTGAAGTTCA GGCAAACCTT 
TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA 
GTGTTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AGTTGGGAAT TATCAGCAAT 
AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC 
CAGCACTGCC TCGTTCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA 
GAGTCCTACT ATAGGCATGC GGCTCAGCTT GTCCCCTCTA ATGGTCAGCC TTACAATCAG 
TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACTA CAATTTTCTA CTACTGCAGA 
AGCATTGCTG TGAAATTCCC TTTCCCAGCT GCCTCTACTA ATCTACAAAA AGCACTTTCT 
AAAGCACTGG AAAGCCGGGA TGAGGTGAAA ACCAAATGGG GTGTTTCTGA CTTCATCAAG 
GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT 
CTTCGAGAGA AGTTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAGAAAGC TTTCAACTCT 
CAGCAGTTAG TTCACGTCAC TGTCATTAAC CTGTTTCAAC TTCATCATCT TCGTGACTTT 
AGCAACGAAA CAGAGCAGCA TAGTTATAGC CAAGATGAGC AGCTGTGTTG GACACAGTTG 
CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTCCA GAACAAGTCT 
CAGGAGGAAT CCTACAATGC CTATCCCCTA CCTGCCGTCA AGGTGTCCAT GGACTGGCTG 
AGACTCAGAC CCAGGGTGTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCG 
TGGCTAATTT CTCTTCTGAA CAGTTTCCAT CCCCATGAAG AAGATCTTTC AAGTACTAAT 
GCAACACCAC TTCCAGAGGA GTTTGAGTTA CAAGGATTCT TGGCTTTGAG ACCTTCTTTC 
AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GAGACAAAGA GGGTCAGCAA 
CGACAAATAC GACAGCAGCG TTTGATCTCT ATAGGCAAAT GGATTGCAGA TAATCAGCCA 
AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAG 
TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA CACAACCATC 
ATAGAGTCCC TGGCAGCTGA CGGGAACCCA GGACTGAAAT CAGTGCTATC TACAGGCCGG 
AATCTAAGCA ACAACTGCGA CCCAGGAGAG AAACCAGTGG TCACCTTCAA AGAGAACATG 
AAGCCACGAG AAGTGAGCAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTAAAATCT 
CAGACAGAGT TAAGAAAGAC TCCAGTGTCT GAAGCCAGGA AAACACCTGT AACTCAAACC 
CCAAGTCAAG CAAGTAACTC CCAGTTCATC CCCATTCATC ACCCTGGAGC CTTCCCTCCT 
CTTCCCAGCC GGCCAGGGTT CCCGCCCCCA ACATATGTTA TCCCCCCTCC TGTGGCATTT 
TCTATGGGCT CAGGTTACAC CTTCCCAGCT GGTGTTTCTG TCCCAGGAAC CTTTCTTCAG 
CCTACAGCTC ACTCTCCACC AGGAAACCAG GTGCAAGCTG GGAAACAGTC CCACATTCCT 
TACAGCCAGC AACGGCCCTC CGGACCAGGG CCAGTGACCC AGGGACCTCA GCAGCCTCAG 
CCACCTTCCC AGCAGCCCCT CCCACCTCTG CCAGCTCCGC CAGCAGCACA GACTGCAGGC 
CAGCTGCAGG TTCAAGCTCT AGCTCAGCAG CAACAGTCCC CTACAAAAGC TGTGCCGGCT 
TTGGGGAAAA GTCCTCCTCA CCACTCTGGA TTCCAGCAGT ATCAACAGGC AGATGCCTCC 
AAACAGCTGT GGAATCCCCC TCAGGTTCAA GGCCCATTAG GGAAAATCAT GCCTGTGAAA 
CAGCCCTACT ACCTTCAAAC CCAAGACCCC ATAAAACTGT TTGAGCCGTC GTTGCAACCT 
CCTGTAAGGC AGCAGCAGCC TCTAGAGAAA ACAATGAAGC CTTTCCCCAT GGAGCCATAT 
AACCATAACC CCTCAGAAGT CAAGATCCCG GAGTTCTACT GGGATTCTTC CTACAGCGCT 
GCTGATAACA GAGCTGTGAT GGCACAGCAA GCCAGTGTGG ACCGCAGGGG CAAACGGTCC 
CCGGGAGTCT TCCGTCCAGA GCAGGACCCT GTGCCCAGGA TGCCATTTGA GAAATCCTTA 
CTGGAGAAGC CCTCAGAGCT CATGTCACAT TCATCCTCTT TCCTGTCCCT CACCGGATTC 
TCCCTCAATC AGGAGCGATA CCCAAATAAC AGTGTGTTCA ATGAGGTATA CGGGAAGAAC 
CTGACAACCA GCTCCAAAGC AGAACTTAAT CCCTCAGTGG CTCCCCAGGA AACCTCACTG 
TACTCGCTTT TTGAAGGGAC TCCATGGTCT CCATCTCTTC CTGCCAGTTC AGATCATTCA 
ACACCAGCCA GCCAATCTCC TCATTCCTCC AACCCAAGCA GCCTGCCAAG CTCCCCTCCA 
ACACACAACC ACAATTCTGT CCCATTCTCC AATTTTGGAC CCATTGGGAC TCCAGATAAC 
AGGGATAGGA GAACTGCAGA CCGGTGGAAA ACTGATAAGC CAGCCATGGG TGGGTTTGGC 
ATCGATTATC TCTCAGCAAC ATCATCCTCT GAGAGCAGTT GGCATCAGGC CAGCACTCCA 
AGTGGCTCCT GGACAGGCCA TGGCCCATCC ATGGAGGATT CCTCTGCTGT CCTCATGGAA 
AGCCTAAAGT CTATCTGGTC CAGTTCCATG ATGCATCCTG GACCTTCCGC TTTGGAGCAG 
CTGTTAATGC AGCAGAAGCA GAAACAGCAG CGGGGCCAAG GCGCCATGAA CCCTCCACAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG7 ( XM_024976349.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832117.1 Bos taurus SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OBa223657
Clone ID Related Accession (Same CDS sequence)	XM_024976349.1
Accession Version	XM_024976349.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3276bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein SMG7 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCTGC AGAGCGCGCA GTATCTCCGG CAGGCAGAAG TCCTGAAGGC TGACATGACA 
GATTCTAAGC TGGGTCCAGC CGAAGTCTGG ACATCCAGGC AGGCTCTGCA GGACCTGTAC 
CAGAAAATGC TTGTTACTGA TTTGGAATAC GCTTTAGACA AGAAAGTAGA ACAGGATCTC 
TGGAATCATG CCTTTAAGAA TCAGATCACA ACACTACAAG GCCAGGCAAA GAATCGAGCA 
AACCCGAATC GGAGTGAAGT TCAGGCAAAC CTTTCTCTGT TCCTAGAGGC AGCTAGTGGC 
TTCTATACTC AGTTATTACA AGAACTGTGT ACAGTGTTTA ATGTAGATTT ACCATGCCGT 
GTGAAGTCTT CCCAGTTGGG AATTATCAGC AATAAACAGA CGCATACCAG CGCCATAGTG 
AAGCCACAGT CTAGCTCCTG TTCCTATATC TGCCAGCACT GCCTCGTTCA CCTTGGAGAC 
ATTGCTCGAT ACAGAAACCA GACCAGCCAG GCAGAGTCCT ACTATAGGCA TGCGGCTCAG 
CTTGTCCCCT CTAATGGTCA GCCTTACAAT CAGTTGGCTA TCTTAGCTTC TTCCAAAGGA 
GACCATCTGA CTACAATTTT CTACTACTGC AGAAGCATTG CTGTGAAATT CCCTTTCCCA 
GCTGCCTCTA CTAATCTACA AAAAGCACTT TCTAAAGCAC TGGAAAGCCG GGATGAGGTG 
AAAACCAAAT GGGGTGTTTC TGACTTCATC AAGGCCTTTA TTAAATTCCA CGGTCATGTG 
TACCTGAGTA AGAGCTTGGA AAAGTTGAGC CCTCTTCGAG AGAAGTTGGA AGAACAGTTT 
AAGAGGCTGC TATTCCAGAA AGCTTTCAAC TCTCAGCAGT TAGTTCACGT CACTGTCATT 
AACCTGTTTC AACTTCATCA TCTTCGTGAC TTTAGCAACG AAACAGAGCA GCATAGTTAT 
AGCCAAGATG AGCAGCTGTG TTGGACACAG TTGCTGGCCC TCTTTATGTC TTTTCTTGGC 
ATCCTGTGCA AGTGTCCTCT CCAGAACAAG TCTCAGGAGG AATCCTACAA TGCCTATCCC 
CTACCTGCCG TCAAGGTGTC CATGGACTGG CTGAGACTCA GACCCAGGGT GTTTCAGGAG 
GCAGTGGTGG ATGAAAGACA GTACATTTGG CCGTGGCTAA TTTCTCTTCT GAACAGTTTC 
CATCCCCATG AAGAAGATCT TTCAAGTACT AATGCAACAC CACTTCCAGA GGAGTTTGAG 
TTACAAGGAT TCTTGGCTTT GAGACCTTCT TTCAGGAACT TGGATTTTTC CAAAGGTCAC 
CAGGGTATTA CAGGAGACAA AGAGGGTCAG CAACGACAAA TACGACAGCA GCGTTTGATC 
TCTATAGGCA AATGGATTGC AGATAATCAG CCAAGGCTGA TTCAGTGTGA AAATGAGGTA 
GGGAAATTGT TGTTTATCAC AGAAATCCCA GAGTTAATAC TGGAAGACCC CAGTGAAGCC 
AAAGAGAACC TCATTCTGCA AGACACAACC ATCATAGAGT CCCTGGCAGC TGACGGGAAC 
CCAGGACTGA AATCAGTGCT ATCTACAGGC CGGAATCTAA GCAACAACTG CGACCCAGGA 
GAGAAACCAG TGGTCACCTT CAAAGAGAAC ATGAAGCCAC GAGAAGTGAG CAGAGACCAA 
GGAAGAAGTT TTCCTCCCAA AGAGGTAAAA TCTCAGACAG AGTTAAGAAA GACTCCAGTG 
TCTGAAGCCA GGAAAACACC TGTAACTCAA ACCCCAAGTC AAGCAAGTAA CTCCCAGTTC 
ATCCCCATTC ATCACCCTGG AGCCTTCCCT CCTCTTCCCA GCCGGCCAGG GTTCCCGCCC 
CCAACATATG TTATCCCCCC TCCTGTGGCA TTTTCTATGG GCTCAGGTTA CACCTTCCCA 
GCTGGTGTTT CTGTCCCAGG AACCTTTCTT CAGCCTACAG CTCACTCTCC ACCAGGAAAC 
CAGGTGCAAG CTGGGAAACA GTCCCACATT CCTTACAGCC AGCAACGGCC CTCCGGACCA 
GGGCCAGTGA CCCAGGGACC TCAGCAGCCT CAGCCACCTT CCCAGCAGCC CCTCCCACCT 
CTGCCAGCTC CGCCAGCAGC ACAGACTGCA GGCCAGCTGC AGGTTCAAGC TCTAGCTCAG 
CAGCAACAGT CCCCTACAAA AGCTGTGCCG GCTTTGGGGA AAAGTCCTCC TCACCACTCT 
GGATTCCAGC AGTATCAACA GGCAGATGCC TCCAAACAGC TGTGGAATCC CCCTCAGGTT 
CAAGGCCCAT TAGGGAAAAT CATGCCTGTG AAACAGCCCT ACTACCTTCA AACCCAAGAC 
CCCATAAAAC TGTTTGAGCC GTCGTTGCAA CCTCCTGTAA GGCAGCAGCA GCCTCTAGAG 
AAAACAATGA AGCCTTTCCC CATGGAGCCA TATAACCATA ACCCCTCAGA AGTCAAGATC 
CCGGAGTTCT ACTGGGATTC TTCCTACAGC GCTGCTGATA ACAGAGCTGT GATGGCACAG 
CAAGCCAGTG TGGACCGCAG GGGCAAACGG TCCCCGGGAG TCTTCCGTCC AGAGCAGGAC 
CCTGTGCCCA GGATGCCATT TGAGAAATCC TTACTGGAGA AGCCCTCAGA GCTCATGTCA 
CATTCATCCT CTTTCCTGTC CCTCACCGGA TTCTCCCTCA ATCAGGAGCG ATACCCAAAT 
AACAGTGTGT TCAATGAGGT ATACGGGAAG AACCTGACAA CCAGCTCCAA AGCAGAACTT 
AATCCCTCAG TGGCTCCCCA GGAAACCTCA CTGTACTCGC TTTTTGAAGG GACTCCATGG 
TCTCCATCTC TTCCTGCCAG TTCAGATCAT TCAACACCAG CCAGCCAATC TCCTCATTCC 
TCCAACCCAA GCAGCCTGCC AAGCTCCCCT CCAACACACA ACCACAATTC TGTCCCATTC 
TCCAATTTTG GACCCATTGG GACTCCAGAT AACAGGGATA GGAGAACTGC AGACCGGTGG 
AAAACTGATA AGCCAGCCAT GGGTGGGTTT GGCATCGATT ATCTCTCAGC AACATCATCC 
TCTGAGAGCA GTTGGCATCA GGCCAGCACT CCAAGTGGCT CCTGGACAGG CCATGGCCCA 
TCCATGGAGG ATTCCTCTGC TGTCCTCATG GAAAGCCTAA AGTCTATCTG GTCCAGTTCC 
ATGATGCATC CTGGACCTTC CGCTTTGGAG CAGCTGTTAA TGCAGCAGAA GCAGAAACAG 
CAGCGGGGCC AAGGCGCCAT GAACCCTCCA CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832117.1
CDS	276..3551
Translation	MSLQSAQYLRQAEVLKADMTDSKLGPAEVWTSRQALQDLYQKMLVTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRANPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQTHTSAIVKPQSSSCSYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNLQKALSKALESRDEVKTKWGVSDFIKAFIKFHGHVYLSKSLEKLSPLREKLEEQFKRLLFQKAFNSQQLVHVTVINLFQLHHLRDFSNETEQHSYSQDEQLCWTQLLALFMSFLGILCKCPLQNKSQEESYNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPHEEDLSSTNATPLPEEFELQGFLALRPSFRNLDFSKGHQGITGDKEGQQRQIRQQRLISIGKWIADNQPRLIQCENEVGKLLFITEIPELILEDPSEAKENLILQDTTIIESLAADGNPGLKSVLSTGRNLSNNCDPGEKPVVTFKENMKPREVSRDQGRSFPPKEVKSQTELRKTPVSEARKTPVTQTPSQASNSQFIPIHHPGAFPPLPSRPGFPPPTYVIPPPVAFSMGSGYTFPAGVSVPGTFLQPTAHSPPGNQVQAGKQSHIPYSQQRPSGPGPVTQGPQQPQPPSQQPLPPLPAPPAAQTAGQLQVQALAQQQQSPTKAVPALGKSPPHHSGFQQYQQADASKQLWNPPQVQGPLGKIMPVKQPYYLQTQDPIKLFEPSLQPPVRQQQPLEKTMKPFPMEPYNHNPSEVKIPEFYWDSSYSAADNRAVMAQQASVDRRGKRSPGVFRPEQDPVPRMPFEKSLLEKPSELMSHSSSFLSLTGFSLNQERYPNNSVFNEVYGKNLTTSSKAELNPSVAPQETSLYSLFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSVPFSNFGPIGTPDNRDRRTADRWKTDKPAMGGFGIDYLSATSSSESSWHQASTPSGSWTGHGPSMEDSSAVLMESLKSIWSSSMMHPGPSALEQLLMQQKQKQQRGQGAMNPPH

Target ORF information:

RefSeq Version	XM_024976349.1
Organism	Bos taurus(cattle)
Definition	Bos taurus SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976349.1

ORF Insert Sequence:

ATGAGCCTGC AGAGCGCGCA GTATCTCCGG CAGGCAGAAG TCCTGAAGGC TGACATGACA 
GATTCTAAGC TGGGTCCAGC CGAAGTCTGG ACATCCAGGC AGGCTCTGCA GGACCTGTAC 
CAGAAAATGC TTGTTACTGA TTTGGAATAC GCTTTAGACA AGAAAGTAGA ACAGGATCTC 
TGGAATCATG CCTTTAAGAA TCAGATCACA ACACTACAAG GCCAGGCAAA GAATCGAGCA 
AACCCGAATC GGAGTGAAGT TCAGGCAAAC CTTTCTCTGT TCCTAGAGGC AGCTAGTGGC 
TTCTATACTC AGTTATTACA AGAACTGTGT ACAGTGTTTA ATGTAGATTT ACCATGCCGT 
GTGAAGTCTT CCCAGTTGGG AATTATCAGC AATAAACAGA CGCATACCAG CGCCATAGTG 
AAGCCACAGT CTAGCTCCTG TTCCTATATC TGCCAGCACT GCCTCGTTCA CCTTGGAGAC 
ATTGCTCGAT ACAGAAACCA GACCAGCCAG GCAGAGTCCT ACTATAGGCA TGCGGCTCAG 
CTTGTCCCCT CTAATGGTCA GCCTTACAAT CAGTTGGCTA TCTTAGCTTC TTCCAAAGGA 
GACCATCTGA CTACAATTTT CTACTACTGC AGAAGCATTG CTGTGAAATT CCCTTTCCCA 
GCTGCCTCTA CTAATCTACA AAAAGCACTT TCTAAAGCAC TGGAAAGCCG GGATGAGGTG 
AAAACCAAAT GGGGTGTTTC TGACTTCATC AAGGCCTTTA TTAAATTCCA CGGTCATGTG 
TACCTGAGTA AGAGCTTGGA AAAGTTGAGC CCTCTTCGAG AGAAGTTGGA AGAACAGTTT 
AAGAGGCTGC TATTCCAGAA AGCTTTCAAC TCTCAGCAGT TAGTTCACGT CACTGTCATT 
AACCTGTTTC AACTTCATCA TCTTCGTGAC TTTAGCAACG AAACAGAGCA GCATAGTTAT 
AGCCAAGATG AGCAGCTGTG TTGGACACAG TTGCTGGCCC TCTTTATGTC TTTTCTTGGC 
ATCCTGTGCA AGTGTCCTCT CCAGAACAAG TCTCAGGAGG AATCCTACAA TGCCTATCCC 
CTACCTGCCG TCAAGGTGTC CATGGACTGG CTGAGACTCA GACCCAGGGT GTTTCAGGAG 
GCAGTGGTGG ATGAAAGACA GTACATTTGG CCGTGGCTAA TTTCTCTTCT GAACAGTTTC 
CATCCCCATG AAGAAGATCT TTCAAGTACT AATGCAACAC CACTTCCAGA GGAGTTTGAG 
TTACAAGGAT TCTTGGCTTT GAGACCTTCT TTCAGGAACT TGGATTTTTC CAAAGGTCAC 
CAGGGTATTA CAGGAGACAA AGAGGGTCAG CAACGACAAA TACGACAGCA GCGTTTGATC 
TCTATAGGCA AATGGATTGC AGATAATCAG CCAAGGCTGA TTCAGTGTGA AAATGAGGTA 
GGGAAATTGT TGTTTATCAC AGAAATCCCA GAGTTAATAC TGGAAGACCC CAGTGAAGCC 
AAAGAGAACC TCATTCTGCA AGACACAACC ATCATAGAGT CCCTGGCAGC TGACGGGAAC 
CCAGGACTGA AATCAGTGCT ATCTACAGGC CGGAATCTAA GCAACAACTG CGACCCAGGA 
GAGAAACCAG TGGTCACCTT CAAAGAGAAC ATGAAGCCAC GAGAAGTGAG CAGAGACCAA 
GGAAGAAGTT TTCCTCCCAA AGAGGTAAAA TCTCAGACAG AGTTAAGAAA GACTCCAGTG 
TCTGAAGCCA GGAAAACACC TGTAACTCAA ACCCCAAGTC AAGCAAGTAA CTCCCAGTTC 
ATCCCCATTC ATCACCCTGG AGCCTTCCCT CCTCTTCCCA GCCGGCCAGG GTTCCCGCCC 
CCAACATATG TTATCCCCCC TCCTGTGGCA TTTTCTATGG GCTCAGGTTA CACCTTCCCA 
GCTGGTGTTT CTGTCCCAGG AACCTTTCTT CAGCCTACAG CTCACTCTCC ACCAGGAAAC 
CAGGTGCAAG CTGGGAAACA GTCCCACATT CCTTACAGCC AGCAACGGCC CTCCGGACCA 
GGGCCAGTGA CCCAGGGACC TCAGCAGCCT CAGCCACCTT CCCAGCAGCC CCTCCCACCT 
CTGCCAGCTC CGCCAGCAGC ACAGACTGCA GGCCAGCTGC AGGTTCAAGC TCTAGCTCAG 
CAGCAACAGT CCCCTACAAA AGCTGTGCCG GCTTTGGGGA AAAGTCCTCC TCACCACTCT 
GGATTCCAGC AGTATCAACA GGCAGATGCC TCCAAACAGC TGTGGAATCC CCCTCAGGTT 
CAAGGCCCAT TAGGGAAAAT CATGCCTGTG AAACAGCCCT ACTACCTTCA AACCCAAGAC 
CCCATAAAAC TGTTTGAGCC GTCGTTGCAA CCTCCTGTAA GGCAGCAGCA GCCTCTAGAG 
AAAACAATGA AGCCTTTCCC CATGGAGCCA TATAACCATA ACCCCTCAGA AGTCAAGATC 
CCGGAGTTCT ACTGGGATTC TTCCTACAGC GCTGCTGATA ACAGAGCTGT GATGGCACAG 
CAAGCCAGTG TGGACCGCAG GGGCAAACGG TCCCCGGGAG TCTTCCGTCC AGAGCAGGAC 
CCTGTGCCCA GGATGCCATT TGAGAAATCC TTACTGGAGA AGCCCTCAGA GCTCATGTCA 
CATTCATCCT CTTTCCTGTC CCTCACCGGA TTCTCCCTCA ATCAGGAGCG ATACCCAAAT 
AACAGTGTGT TCAATGAGGT ATACGGGAAG AACCTGACAA CCAGCTCCAA AGCAGAACTT 
AATCCCTCAG TGGCTCCCCA GGAAACCTCA CTGTACTCGC TTTTTGAAGG GACTCCATGG 
TCTCCATCTC TTCCTGCCAG TTCAGATCAT TCAACACCAG CCAGCCAATC TCCTCATTCC 
TCCAACCCAA GCAGCCTGCC AAGCTCCCCT CCAACACACA ACCACAATTC TGTCCCATTC 
TCCAATTTTG GACCCATTGG GACTCCAGAT AACAGGGATA GGAGAACTGC AGACCGGTGG 
AAAACTGATA AGCCAGCCAT GGGTGGGTTT GGCATCGATT ATCTCTCAGC AACATCATCC 
TCTGAGAGCA GTTGGCATCA GGCCAGCACT CCAAGTGGCT CCTGGACAGG CCATGGCCCA 
TCCATGGAGG ATTCCTCTGC TGTCCTCATG GAAAGCCTAA AGTCTATCTG GTCCAGTTCC 
ATGATGCATC CTGGACCTTC CGCTTTGGAG CAGCTGTTAA TGCAGCAGAA GCAGAAACAG 
CAGCGGGGCC AAGGCGCCAT GAACCCTCCA CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG7 ( XM_024976350.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832118.1 Bos taurus SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OBa223658
Clone ID Related Accession (Same CDS sequence)	XM_024976350.1
Accession Version	XM_024976350.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3273bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein SMG7 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
CAGATGAGGA CCGGAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG 
GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCCGA AGTCTGGACA 
TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTTG TTACTGATTT GGAATACGCT 
TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCATGCCT TTAAGAATCA GATCACAACA 
CTACAAGGCC AGGCAAAGAA TCGAGCAAAC CCGAATCGGA GTGAAGTTCA GGCAAACCTT 
TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA 
GTGTTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AGTTGGGAAT TATCAGCAAT 
AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC 
CAGCACTGCC TCGTTCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA 
GAGTCCTACT ATAGGCATGC GGCTCAGCTT GTCCCCTCTA ATGGTCAGCC TTACAATCAG 
TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACTA CAATTTTCTA CTACTGCAGA 
AGCATTGCTG TGAAATTCCC TTTCCCAGCT GCCTCTACTA ATCTACAAAA AGCACTTTCT 
AAAGCACTGG AAAGCCGGGA TGAGGTGAAA ACCAAATGGG GTGTTTCTGA CTTCATCAAG 
GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT 
CTTCGAGAGA AGTTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAGAAAGC TTTCAACTCT 
CAGCAGTTAG TTCACGTCAC TGTCATTAAC CTGTTTCAAC TTCATCATCT TCGTGACTTT 
AGCAACGAAA CAGAGCAGCA TAGTTATAGC CAAGATGAGC AGCTGTGTTG GACACAGTTG 
CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTCCA GAACAAGTCT 
CAGGAGGAAT CCTACAATGC CTATCCCCTA CCTGCCGTCA AGGTGTCCAT GGACTGGCTG 
AGACTCAGAC CCAGGGTGTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCG 
TGGCTAATTT CTCTTCTGAA CAGTTTCCAT CCCCATGAAG AAGATCTTTC AAGTACTAAT 
GCAACACCAC TTCCAGAGGA GTTTGAGTTA CAAGGATTCT TGGCTTTGAG ACCTTCTTTC 
AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GAGACAAAGA GGGTCAGCAA 
CGACAAATAC GACAGCAGCG TTTGATCTCT ATAGGCAAAT GGATTGCAGA TAATCAGCCA 
AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAG 
TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA CACAACCATC 
ATAGAGTCCC TGGCAGCTGA CGGGAACCCA GGACTGAAAT CAGTGCTATC TACAGGCCGG 
AATCTAAGCA ACAACTGCGA CCCAGGAGAG AAACCAGTGG TCACCTTCAA AGAGAACATG 
AAGCCACGAG AAGTGAGCAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTAAAATCT 
CAGACAGAGT TAAGAAAGAC TCCAGTGTCT GAAGCCAGGA AAACACCTGT AACTCAAACC 
CCAAGTCAAG CAAGTAACTC CCAGTTCATC CCCATTCATC ACCCTGGAGC CTTCCCTCCT 
CTTCCCAGCC GGCCAGGAAC CTTTCTTCAG CCTACAGCTC ACTCTCCACC AGGAAACCAG 
GTGCAAGCTG GGAAACAGTC CCACATTCCT TACAGCCAGC AACGGCCCTC CGGACCAGGG 
CCAGTGACCC AGGGACCTCA GCAGCCTCAG CCACCTTCCC AGCAGCCCCT CCCACCTCTG 
CCAGCTCCGC CAGCAGCACA GACTGCAGGC CAGCTGCAGG TTCAAGCTCT AGCTCAGCAG 
CAACAGTCCC CTACAAAAGC TGTGCCGGCT TTGGGGAAAA GTCCTCCTCA CCACTCTGGA 
TTCCAGCAGT ATCAACAGGC AGATGCCTCC AAACAGCTGT GGAATCCCCC TCAGGTTCAA 
GGCCCATTAG GGAAAATCAT GCCTGTGAAA CAGCCCTACT ACCTTCAAAC CCAAGACCCC 
ATAAAACTGT TTGAGCCGTC GTTGCAACCT CCTGTAAGGC AGCAGCAGCC TCTAGAGAAA 
ACAATGAAGC CTTTCCCCAT GGAGCCATAT AACCATAACC CCTCAGAAGT CAAGATCCCG 
GAGTTCTACT GGGATTCTTC CTACAGCGCT GCTGATAACA GAGCTGTGAT GGCACAGCAA 
GCCAGTGTGG ACCGCAGGGG CAAACGGTCC CCGGGAGTCT TCCGTCCAGA GCAGGACCCT 
GTGCCCAGGA TGCCATTTGA GAAATCCTTA CTGGAGAAGC CCTCAGAGCT CATGTCACAT 
TCATCCTCTT TCCTGTCCCT CACCGGATTC TCCCTCAATC AGGAGCGATA CCCAAATAAC 
AGTGTGTTCA ATGAGGTATA CGGGAAGAAC CTGACAACCA GCTCCAAAGC AGAACTTAAT 
CCCTCAGTGG CTCCCCAGGA AACCTCACTG TACTCGCTTT TTGAAGGGAC TCCATGGTCT 
CCATCTCTTC CTGCCAGTTC AGATCATTCA ACACCAGCCA GCCAATCTCC TCATTCCTCC 
AACCCAAGCA GCCTGCCAAG CTCCCCTCCA ACACACAACC ACAATTCTGT CCCATTCTCC 
AATTTTGGAC CCATTGGGAC TCCAGATAAC AGGGATAGGA GAACTGCAGA CCGGTGGAAA 
ACTGATAAGC CAGCCATGGG TGGGTTTGGC ATCGATTATC TCTCAGCAAC ATCATCCTCT 
GAGAGCAGTT GGCATCAGGC CAGCACTCCA AGTGGCTCCT GGACAGGCCA TGGCCCATCC 
ATGGAGGATT CCTCTGCTGT CCTCATGGAA AGCCTAAAGT CTATCTGGTC CAGTTCCATG 
ATGCATCCTG GACCTTCCGC TTTGGAGCAG CTGTTAATGC AGCAGAAGCA GAAACAGCAG 
CGGGGCCAAG GCGCCATGAA CCCTCCACAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832118.1
CDS	149..3421
Translation	MRNWMFLQIVIVGENVSFKSQMRTGNLKSEEHLKSSNIRQAEVLKADMTDSKLGPAEVWTSRQALQDLYQKMLVTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRANPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQTHTSAIVKPQSSSCSYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNLQKALSKALESRDEVKTKWGVSDFIKAFIKFHGHVYLSKSLEKLSPLREKLEEQFKRLLFQKAFNSQQLVHVTVINLFQLHHLRDFSNETEQHSYSQDEQLCWTQLLALFMSFLGILCKCPLQNKSQEESYNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPHEEDLSSTNATPLPEEFELQGFLALRPSFRNLDFSKGHQGITGDKEGQQRQIRQQRLISIGKWIADNQPRLIQCENEVGKLLFITEIPELILEDPSEAKENLILQDTTIIESLAADGNPGLKSVLSTGRNLSNNCDPGEKPVVTFKENMKPREVSRDQGRSFPPKEVKSQTELRKTPVSEARKTPVTQTPSQASNSQFIPIHHPGAFPPLPSRPGTFLQPTAHSPPGNQVQAGKQSHIPYSQQRPSGPGPVTQGPQQPQPPSQQPLPPLPAPPAAQTAGQLQVQALAQQQQSPTKAVPALGKSPPHHSGFQQYQQADASKQLWNPPQVQGPLGKIMPVKQPYYLQTQDPIKLFEPSLQPPVRQQQPLEKTMKPFPMEPYNHNPSEVKIPEFYWDSSYSAADNRAVMAQQASVDRRGKRSPGVFRPEQDPVPRMPFEKSLLEKPSELMSHSSSFLSLTGFSLNQERYPNNSVFNEVYGKNLTTSSKAELNPSVAPQETSLYSLFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSVPFSNFGPIGTPDNRDRRTADRWKTDKPAMGGFGIDYLSATSSSESSWHQASTPSGSWTGHGPSMEDSSAVLMESLKSIWSSSMMHPGPSALEQLLMQQKQKQQRGQGAMNPPH

Target ORF information:

RefSeq Version	XM_024976350.1
Organism	Bos taurus(cattle)
Definition	Bos taurus SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976350.1

ORF Insert Sequence:

ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
CAGATGAGGA CCGGAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG 
GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCCGA AGTCTGGACA 
TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTTG TTACTGATTT GGAATACGCT 
TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCATGCCT TTAAGAATCA GATCACAACA 
CTACAAGGCC AGGCAAAGAA TCGAGCAAAC CCGAATCGGA GTGAAGTTCA GGCAAACCTT 
TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA 
GTGTTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AGTTGGGAAT TATCAGCAAT 
AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC 
CAGCACTGCC TCGTTCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA 
GAGTCCTACT ATAGGCATGC GGCTCAGCTT GTCCCCTCTA ATGGTCAGCC TTACAATCAG 
TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACTA CAATTTTCTA CTACTGCAGA 
AGCATTGCTG TGAAATTCCC TTTCCCAGCT GCCTCTACTA ATCTACAAAA AGCACTTTCT 
AAAGCACTGG AAAGCCGGGA TGAGGTGAAA ACCAAATGGG GTGTTTCTGA CTTCATCAAG 
GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT 
CTTCGAGAGA AGTTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAGAAAGC TTTCAACTCT 
CAGCAGTTAG TTCACGTCAC TGTCATTAAC CTGTTTCAAC TTCATCATCT TCGTGACTTT 
AGCAACGAAA CAGAGCAGCA TAGTTATAGC CAAGATGAGC AGCTGTGTTG GACACAGTTG 
CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTCCA GAACAAGTCT 
CAGGAGGAAT CCTACAATGC CTATCCCCTA CCTGCCGTCA AGGTGTCCAT GGACTGGCTG 
AGACTCAGAC CCAGGGTGTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCG 
TGGCTAATTT CTCTTCTGAA CAGTTTCCAT CCCCATGAAG AAGATCTTTC AAGTACTAAT 
GCAACACCAC TTCCAGAGGA GTTTGAGTTA CAAGGATTCT TGGCTTTGAG ACCTTCTTTC 
AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GAGACAAAGA GGGTCAGCAA 
CGACAAATAC GACAGCAGCG TTTGATCTCT ATAGGCAAAT GGATTGCAGA TAATCAGCCA 
AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAG 
TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA CACAACCATC 
ATAGAGTCCC TGGCAGCTGA CGGGAACCCA GGACTGAAAT CAGTGCTATC TACAGGCCGG 
AATCTAAGCA ACAACTGCGA CCCAGGAGAG AAACCAGTGG TCACCTTCAA AGAGAACATG 
AAGCCACGAG AAGTGAGCAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTAAAATCT 
CAGACAGAGT TAAGAAAGAC TCCAGTGTCT GAAGCCAGGA AAACACCTGT AACTCAAACC 
CCAAGTCAAG CAAGTAACTC CCAGTTCATC CCCATTCATC ACCCTGGAGC CTTCCCTCCT 
CTTCCCAGCC GGCCAGGAAC CTTTCTTCAG CCTACAGCTC ACTCTCCACC AGGAAACCAG 
GTGCAAGCTG GGAAACAGTC CCACATTCCT TACAGCCAGC AACGGCCCTC CGGACCAGGG 
CCAGTGACCC AGGGACCTCA GCAGCCTCAG CCACCTTCCC AGCAGCCCCT CCCACCTCTG 
CCAGCTCCGC CAGCAGCACA GACTGCAGGC CAGCTGCAGG TTCAAGCTCT AGCTCAGCAG 
CAACAGTCCC CTACAAAAGC TGTGCCGGCT TTGGGGAAAA GTCCTCCTCA CCACTCTGGA 
TTCCAGCAGT ATCAACAGGC AGATGCCTCC AAACAGCTGT GGAATCCCCC TCAGGTTCAA 
GGCCCATTAG GGAAAATCAT GCCTGTGAAA CAGCCCTACT ACCTTCAAAC CCAAGACCCC 
ATAAAACTGT TTGAGCCGTC GTTGCAACCT CCTGTAAGGC AGCAGCAGCC TCTAGAGAAA 
ACAATGAAGC CTTTCCCCAT GGAGCCATAT AACCATAACC CCTCAGAAGT CAAGATCCCG 
GAGTTCTACT GGGATTCTTC CTACAGCGCT GCTGATAACA GAGCTGTGAT GGCACAGCAA 
GCCAGTGTGG ACCGCAGGGG CAAACGGTCC CCGGGAGTCT TCCGTCCAGA GCAGGACCCT 
GTGCCCAGGA TGCCATTTGA GAAATCCTTA CTGGAGAAGC CCTCAGAGCT CATGTCACAT 
TCATCCTCTT TCCTGTCCCT CACCGGATTC TCCCTCAATC AGGAGCGATA CCCAAATAAC 
AGTGTGTTCA ATGAGGTATA CGGGAAGAAC CTGACAACCA GCTCCAAAGC AGAACTTAAT 
CCCTCAGTGG CTCCCCAGGA AACCTCACTG TACTCGCTTT TTGAAGGGAC TCCATGGTCT 
CCATCTCTTC CTGCCAGTTC AGATCATTCA ACACCAGCCA GCCAATCTCC TCATTCCTCC 
AACCCAAGCA GCCTGCCAAG CTCCCCTCCA ACACACAACC ACAATTCTGT CCCATTCTCC 
AATTTTGGAC CCATTGGGAC TCCAGATAAC AGGGATAGGA GAACTGCAGA CCGGTGGAAA 
ACTGATAAGC CAGCCATGGG TGGGTTTGGC ATCGATTATC TCTCAGCAAC ATCATCCTCT 
GAGAGCAGTT GGCATCAGGC CAGCACTCCA AGTGGCTCCT GGACAGGCCA TGGCCCATCC 
ATGGAGGATT CCTCTGCTGT CCTCATGGAA AGCCTAAAGT CTATCTGGTC CAGTTCCATG 
ATGCATCCTG GACCTTCCGC TTTGGAGCAG CTGTTAATGC AGCAGAAGCA GAAACAGCAG 
CGGGGCCAAG GCGCCATGAA CCCTCCACAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG7 ( XM_024976351.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832119.1 Bos taurus SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OBa223659
Clone ID Related Accession (Same CDS sequence)	XM_024976351.1
Accession Version	XM_024976351.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3186bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein SMG7 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCTGC AGAGCGCGCA GTATCTCCGG CAGGCAGAAG TCCTGAAGGC TGACATGACA 
GATTCTAAGC TGGGTCCAGC CGAAGTCTGG ACATCCAGGC AGGCTCTGCA GGACCTGTAC 
CAGAAAATGC TTGTTACTGA TTTGGAATAC GCTTTAGACA AGAAAGTAGA ACAGGATCTC 
TGGAATCATG CCTTTAAGAA TCAGATCACA ACACTACAAG GCCAGGCAAA GAATCGAGCA 
AACCCGAATC GGAGTGAAGT TCAGGCAAAC CTTTCTCTGT TCCTAGAGGC AGCTAGTGGC 
TTCTATACTC AGTTATTACA AGAACTGTGT ACAGTGTTTA ATGTAGATTT ACCATGCCGT 
GTGAAGTCTT CCCAGTTGGG AATTATCAGC AATAAACAGA CGCATACCAG CGCCATAGTG 
AAGCCACAGT CTAGCTCCTG TTCCTATATC TGCCAGCACT GCCTCGTTCA CCTTGGAGAC 
ATTGCTCGAT ACAGAAACCA GACCAGCCAG GCAGAGTCCT ACTATAGGCA TGCGGCTCAG 
CTTGTCCCCT CTAATGGTCA GCCTTACAAT CAGTTGGCTA TCTTAGCTTC TTCCAAAGGA 
GACCATCTGA CTACAATTTT CTACTACTGC AGAAGCATTG CTGTGAAATT CCCTTTCCCA 
GCTGCCTCTA CTAATCTACA AAAAGCACTT TCTAAAGCAC TGGAAAGCCG GGATGAGGTG 
AAAACCAAAT GGGGTGTTTC TGACTTCATC AAGGCCTTTA TTAAATTCCA CGGTCATGTG 
TACCTGAGTA AGAGCTTGGA AAAGTTGAGC CCTCTTCGAG AGAAGTTGGA AGAACAGTTT 
AAGAGGCTGC TATTCCAGAA AGCTTTCAAC TCTCAGCAGT TAGTTCACGT CACTGTCATT 
AACCTGTTTC AACTTCATCA TCTTCGTGAC TTTAGCAACG AAACAGAGCA GCATAGTTAT 
AGCCAAGATG AGCAGCTGTG TTGGACACAG TTGCTGGCCC TCTTTATGTC TTTTCTTGGC 
ATCCTGTGCA AGTGTCCTCT CCAGAACAAG TCTCAGGAGG AATCCTACAA TGCCTATCCC 
CTACCTGCCG TCAAGGTGTC CATGGACTGG CTGAGACTCA GACCCAGGGT GTTTCAGGAG 
GCAGTGGTGG ATGAAAGACA GTACATTTGG CCGTGGCTAA TTTCTCTTCT GAACAGTTTC 
CATCCCCATG AAGAAGATCT TTCAAGTACT AATGCAACAC CACTTCCAGA GGAGTTTGAG 
TTACAAGGAT TCTTGGCTTT GAGACCTTCT TTCAGGAACT TGGATTTTTC CAAAGGTCAC 
CAGGGTATTA CAGGAGACAA AGAGGGTCAG CAACGACAAA TACGACAGCA GCGTTTGATC 
TCTATAGGCA AATGGATTGC AGATAATCAG CCAAGGCTGA TTCAGTGTGA AAATGAGGTA 
GGGAAATTGT TGTTTATCAC AGAAATCCCA GAGTTAATAC TGGAAGACCC CAGTGAAGCC 
AAAGAGAACC TCATTCTGCA AGACACAACC ATCATAGAGT CCCTGGCAGC TGACGGGAAC 
CCAGGACTGA AATCAGTGCT ATCTACAGGC CGGAATCTAA GCAACAACTG CGACCCAGGA 
GAGAAACCAG TGGTCACCTT CAAAGAGAAC ATGAAGCCAC GAGAAGTGAG CAGAGACCAA 
GGAAGAAGTT TTCCTCCCAA AGAGGTAAAA TCTCAGACAG AGTTAAGAAA GACTCCAGTG 
TCTGAAGCCA GGAAAACACC TGTAACTCAA ACCCCAAGTC AAGCAAGTAA CTCCCAGTTC 
ATCCCCATTC ATCACCCTGG AGCCTTCCCT CCTCTTCCCA GCCGGCCAGG AACCTTTCTT 
CAGCCTACAG CTCACTCTCC ACCAGGAAAC CAGGTGCAAG CTGGGAAACA GTCCCACATT 
CCTTACAGCC AGCAACGGCC CTCCGGACCA GGGCCAGTGA CCCAGGGACC TCAGCAGCCT 
CAGCCACCTT CCCAGCAGCC CCTCCCACCT CTGCCAGCTC CGCCAGCAGC ACAGACTGCA 
GGCCAGCTGC AGGTTCAAGC TCTAGCTCAG CAGCAACAGT CCCCTACAAA AGCTGTGCCG 
GCTTTGGGGA AAAGTCCTCC TCACCACTCT GGATTCCAGC AGTATCAACA GGCAGATGCC 
TCCAAACAGC TGTGGAATCC CCCTCAGGTT CAAGGCCCAT TAGGGAAAAT CATGCCTGTG 
AAACAGCCCT ACTACCTTCA AACCCAAGAC CCCATAAAAC TGTTTGAGCC GTCGTTGCAA 
CCTCCTGTAA GGCAGCAGCA GCCTCTAGAG AAAACAATGA AGCCTTTCCC CATGGAGCCA 
TATAACCATA ACCCCTCAGA AGTCAAGATC CCGGAGTTCT ACTGGGATTC TTCCTACAGC 
GCTGCTGATA ACAGAGCTGT GATGGCACAG CAAGCCAGTG TGGACCGCAG GGGCAAACGG 
TCCCCGGGAG TCTTCCGTCC AGAGCAGGAC CCTGTGCCCA GGATGCCATT TGAGAAATCC 
TTACTGGAGA AGCCCTCAGA GCTCATGTCA CATTCATCCT CTTTCCTGTC CCTCACCGGA 
TTCTCCCTCA ATCAGGAGCG ATACCCAAAT AACAGTGTGT TCAATGAGGT ATACGGGAAG 
AACCTGACAA CCAGCTCCAA AGCAGAACTT AATCCCTCAG TGGCTCCCCA GGAAACCTCA 
CTGTACTCGC TTTTTGAAGG GACTCCATGG TCTCCATCTC TTCCTGCCAG TTCAGATCAT 
TCAACACCAG CCAGCCAATC TCCTCATTCC TCCAACCCAA GCAGCCTGCC AAGCTCCCCT 
CCAACACACA ACCACAATTC TGTCCCATTC TCCAATTTTG GACCCATTGG GACTCCAGAT 
AACAGGGATA GGAGAACTGC AGACCGGTGG AAAACTGATA AGCCAGCCAT GGGTGGGTTT 
GGCATCGATT ATCTCTCAGC AACATCATCC TCTGAGAGCA GTTGGCATCA GGCCAGCACT 
CCAAGTGGCT CCTGGACAGG CCATGGCCCA TCCATGGAGG ATTCCTCTGC TGTCCTCATG 
GAAAGCCTAA AGTCTATCTG GTCCAGTTCC ATGATGCATC CTGGACCTTC CGCTTTGGAG 
CAGCTGTTAA TGCAGCAGAA GCAGAAACAG CAGCGGGGCC AAGGCGCCAT GAACCCTCCA 
CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832119.1
CDS	196..3381
Translation	MSLQSAQYLRQAEVLKADMTDSKLGPAEVWTSRQALQDLYQKMLVTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRANPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQTHTSAIVKPQSSSCSYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNLQKALSKALESRDEVKTKWGVSDFIKAFIKFHGHVYLSKSLEKLSPLREKLEEQFKRLLFQKAFNSQQLVHVTVINLFQLHHLRDFSNETEQHSYSQDEQLCWTQLLALFMSFLGILCKCPLQNKSQEESYNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPHEEDLSSTNATPLPEEFELQGFLALRPSFRNLDFSKGHQGITGDKEGQQRQIRQQRLISIGKWIADNQPRLIQCENEVGKLLFITEIPELILEDPSEAKENLILQDTTIIESLAADGNPGLKSVLSTGRNLSNNCDPGEKPVVTFKENMKPREVSRDQGRSFPPKEVKSQTELRKTPVSEARKTPVTQTPSQASNSQFIPIHHPGAFPPLPSRPGTFLQPTAHSPPGNQVQAGKQSHIPYSQQRPSGPGPVTQGPQQPQPPSQQPLPPLPAPPAAQTAGQLQVQALAQQQQSPTKAVPALGKSPPHHSGFQQYQQADASKQLWNPPQVQGPLGKIMPVKQPYYLQTQDPIKLFEPSLQPPVRQQQPLEKTMKPFPMEPYNHNPSEVKIPEFYWDSSYSAADNRAVMAQQASVDRRGKRSPGVFRPEQDPVPRMPFEKSLLEKPSELMSHSSSFLSLTGFSLNQERYPNNSVFNEVYGKNLTTSSKAELNPSVAPQETSLYSLFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSVPFSNFGPIGTPDNRDRRTADRWKTDKPAMGGFGIDYLSATSSSESSWHQASTPSGSWTGHGPSMEDSSAVLMESLKSIWSSSMMHPGPSALEQLLMQQKQKQQRGQGAMNPPH

Target ORF information:

RefSeq Version	XM_024976351.1
Organism	Bos taurus(cattle)
Definition	Bos taurus SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976351.1

ORF Insert Sequence:

ATGAGCCTGC AGAGCGCGCA GTATCTCCGG CAGGCAGAAG TCCTGAAGGC TGACATGACA 
GATTCTAAGC TGGGTCCAGC CGAAGTCTGG ACATCCAGGC AGGCTCTGCA GGACCTGTAC 
CAGAAAATGC TTGTTACTGA TTTGGAATAC GCTTTAGACA AGAAAGTAGA ACAGGATCTC 
TGGAATCATG CCTTTAAGAA TCAGATCACA ACACTACAAG GCCAGGCAAA GAATCGAGCA 
AACCCGAATC GGAGTGAAGT TCAGGCAAAC CTTTCTCTGT TCCTAGAGGC AGCTAGTGGC 
TTCTATACTC AGTTATTACA AGAACTGTGT ACAGTGTTTA ATGTAGATTT ACCATGCCGT 
GTGAAGTCTT CCCAGTTGGG AATTATCAGC AATAAACAGA CGCATACCAG CGCCATAGTG 
AAGCCACAGT CTAGCTCCTG TTCCTATATC TGCCAGCACT GCCTCGTTCA CCTTGGAGAC 
ATTGCTCGAT ACAGAAACCA GACCAGCCAG GCAGAGTCCT ACTATAGGCA TGCGGCTCAG 
CTTGTCCCCT CTAATGGTCA GCCTTACAAT CAGTTGGCTA TCTTAGCTTC TTCCAAAGGA 
GACCATCTGA CTACAATTTT CTACTACTGC AGAAGCATTG CTGTGAAATT CCCTTTCCCA 
GCTGCCTCTA CTAATCTACA AAAAGCACTT TCTAAAGCAC TGGAAAGCCG GGATGAGGTG 
AAAACCAAAT GGGGTGTTTC TGACTTCATC AAGGCCTTTA TTAAATTCCA CGGTCATGTG 
TACCTGAGTA AGAGCTTGGA AAAGTTGAGC CCTCTTCGAG AGAAGTTGGA AGAACAGTTT 
AAGAGGCTGC TATTCCAGAA AGCTTTCAAC TCTCAGCAGT TAGTTCACGT CACTGTCATT 
AACCTGTTTC AACTTCATCA TCTTCGTGAC TTTAGCAACG AAACAGAGCA GCATAGTTAT 
AGCCAAGATG AGCAGCTGTG TTGGACACAG TTGCTGGCCC TCTTTATGTC TTTTCTTGGC 
ATCCTGTGCA AGTGTCCTCT CCAGAACAAG TCTCAGGAGG AATCCTACAA TGCCTATCCC 
CTACCTGCCG TCAAGGTGTC CATGGACTGG CTGAGACTCA GACCCAGGGT GTTTCAGGAG 
GCAGTGGTGG ATGAAAGACA GTACATTTGG CCGTGGCTAA TTTCTCTTCT GAACAGTTTC 
CATCCCCATG AAGAAGATCT TTCAAGTACT AATGCAACAC CACTTCCAGA GGAGTTTGAG 
TTACAAGGAT TCTTGGCTTT GAGACCTTCT TTCAGGAACT TGGATTTTTC CAAAGGTCAC 
CAGGGTATTA CAGGAGACAA AGAGGGTCAG CAACGACAAA TACGACAGCA GCGTTTGATC 
TCTATAGGCA AATGGATTGC AGATAATCAG CCAAGGCTGA TTCAGTGTGA AAATGAGGTA 
GGGAAATTGT TGTTTATCAC AGAAATCCCA GAGTTAATAC TGGAAGACCC CAGTGAAGCC 
AAAGAGAACC TCATTCTGCA AGACACAACC ATCATAGAGT CCCTGGCAGC TGACGGGAAC 
CCAGGACTGA AATCAGTGCT ATCTACAGGC CGGAATCTAA GCAACAACTG CGACCCAGGA 
GAGAAACCAG TGGTCACCTT CAAAGAGAAC ATGAAGCCAC GAGAAGTGAG CAGAGACCAA 
GGAAGAAGTT TTCCTCCCAA AGAGGTAAAA TCTCAGACAG AGTTAAGAAA GACTCCAGTG 
TCTGAAGCCA GGAAAACACC TGTAACTCAA ACCCCAAGTC AAGCAAGTAA CTCCCAGTTC 
ATCCCCATTC ATCACCCTGG AGCCTTCCCT CCTCTTCCCA GCCGGCCAGG AACCTTTCTT 
CAGCCTACAG CTCACTCTCC ACCAGGAAAC CAGGTGCAAG CTGGGAAACA GTCCCACATT 
CCTTACAGCC AGCAACGGCC CTCCGGACCA GGGCCAGTGA CCCAGGGACC TCAGCAGCCT 
CAGCCACCTT CCCAGCAGCC CCTCCCACCT CTGCCAGCTC CGCCAGCAGC ACAGACTGCA 
GGCCAGCTGC AGGTTCAAGC TCTAGCTCAG CAGCAACAGT CCCCTACAAA AGCTGTGCCG 
GCTTTGGGGA AAAGTCCTCC TCACCACTCT GGATTCCAGC AGTATCAACA GGCAGATGCC 
TCCAAACAGC TGTGGAATCC CCCTCAGGTT CAAGGCCCAT TAGGGAAAAT CATGCCTGTG 
AAACAGCCCT ACTACCTTCA AACCCAAGAC CCCATAAAAC TGTTTGAGCC GTCGTTGCAA 
CCTCCTGTAA GGCAGCAGCA GCCTCTAGAG AAAACAATGA AGCCTTTCCC CATGGAGCCA 
TATAACCATA ACCCCTCAGA AGTCAAGATC CCGGAGTTCT ACTGGGATTC TTCCTACAGC 
GCTGCTGATA ACAGAGCTGT GATGGCACAG CAAGCCAGTG TGGACCGCAG GGGCAAACGG 
TCCCCGGGAG TCTTCCGTCC AGAGCAGGAC CCTGTGCCCA GGATGCCATT TGAGAAATCC 
TTACTGGAGA AGCCCTCAGA GCTCATGTCA CATTCATCCT CTTTCCTGTC CCTCACCGGA 
TTCTCCCTCA ATCAGGAGCG ATACCCAAAT AACAGTGTGT TCAATGAGGT ATACGGGAAG 
AACCTGACAA CCAGCTCCAA AGCAGAACTT AATCCCTCAG TGGCTCCCCA GGAAACCTCA 
CTGTACTCGC TTTTTGAAGG GACTCCATGG TCTCCATCTC TTCCTGCCAG TTCAGATCAT 
TCAACACCAG CCAGCCAATC TCCTCATTCC TCCAACCCAA GCAGCCTGCC AAGCTCCCCT 
CCAACACACA ACCACAATTC TGTCCCATTC TCCAATTTTG GACCCATTGG GACTCCAGAT 
AACAGGGATA GGAGAACTGC AGACCGGTGG AAAACTGATA AGCCAGCCAT GGGTGGGTTT 
GGCATCGATT ATCTCTCAGC AACATCATCC TCTGAGAGCA GTTGGCATCA GGCCAGCACT 
CCAAGTGGCT CCTGGACAGG CCATGGCCCA TCCATGGAGG ATTCCTCTGC TGTCCTCATG 
GAAAGCCTAA AGTCTATCTG GTCCAGTTCC ATGATGCATC CTGGACCTTC CGCTTTGGAG 
CAGCTGTTAA TGCAGCAGAA GCAGAAACAG CAGCGGGGCC AAGGCGCCAT GAACCCTCCA 
CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG7 ( XM_010813526.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010811828.2 Bos taurus SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa93045
Clone ID Related Accession (Same CDS sequence)	XM_010813526.2
Accession Version	XM_010813526.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3513bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein SMG7 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104440.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010813526.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
CAGATGAGGA CCGGAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG 
GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCCGA AGTCTGGACA 
TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTTG TTACTGATTT GGAATACGCT 
TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCATGCCT TTAAGAATCA GATCACAACA 
CTACAAGGCC AGGCAAAGAA TCGAGCAAAC CCGAATCGGA GTGAAGTTCA GGCAAACCTT 
TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA 
GTGTTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AGTTGGGAAT TATCAGCAAT 
AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC 
CAGCACTGCC TCGTTCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA 
GAGTCCTACT ATAGGCATGC GGCTCAGCTT GTCCCCTCTA ATGGTCAGCC TTACAATCAG 
TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACTA CAATTTTCTA CTACTGCAGA 
AGCATTGCTG TGAAATTCCC TTTCCCAGCT GCCTCTACTA ATCTACAAAA AGCACTTTCT 
AAAGCACTGG AAAGCCGGGA TGAGGTGAAA ACCAAATGGG GTGTTTCTGA CTTCATCAAG 
GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT 
CTTCGAGAGA AGTTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAGAAAGC TTTCAACTCT 
CAGCAGTTAG TTCACGTCAC TGTCATTAAC CTGTTTCAAC TTCATCATCT TCGTGACTTT 
AGCAACGAAA CGGAGCAGCA TAGTTATAGC CAAGATGAGC AGCTGTGTTG GACACAGTTG 
CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTCCA GAACAAGTCT 
CAGGAGGAAT CCTACAATGC CTATCCCCTA CCTGCCGTCA AGGTGTCCAT GGACTGGCTG 
AGACTCAGAC CCAGGGTGTT TCAGGAAGCA GTGGTGGATG AAAGACAGTA CATTTGGCCG 
TGGCTAATTT CTCTTCTGAA CAGTTTCCAT CCCCATGAAG AAGATCTTTC AAGTACTAAT 
GCAACACCAC TTCCAGAGGA GTTTGAGTTA CAAGGATTCT TGGCTTTGAG ACCTTCTTTC 
AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GAGACAAAGA GGGTCAGCAA 
CGACAAATAC GACAGCAGCG TTTGATCTCT ATAGGCAAAT GGATTGCAGA TAATCAGCCA 
AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAG 
TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA CACAACCATC 
ATAGAGTCCC TGGCAGCTGA CGGGAACCCA GGACTGAAAT CAGTGCTATC TACAGGCCGG 
AATCTAAGCA ACAACTGCGA CCCAGGAGAG AAACCAGTGG TCACCTTCAA AGAGAACATG 
AAGCCACGAG AAGTGAGCAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTAAAATCT 
CAGACAGAGT TAAGAAAGAC TCCAGTGTCT GAAGCCAGGA AAACACCTGT AACTCAAACC 
CCAAGTCAAG CAAGTAACTC CCAGTTCATC CCCATTCATC ACCCTGGAGC CTTCCCTCCT 
CTTCCCAGCC GGCCAGGGTT CCCGCCCCCA ACATATGTTA TCCCCCCTCC TGTGGCATTT 
TCTATGGGCT CAGGTTACAC CTTCCCAGCT GGTGTTTCTG TCCCAGGAAC CTTTCTTCAG 
CCTACAGCTC ACTCTCCACC AGGAAACCAG GTGCAAGCTG GGAAACAGTC CCACATTCCT 
TACAGCCAGC AACGGCCCTC CGGACCAGGG CCAGTGACCC AGGGACCTCA GCAGCCTCAG 
CCACCTTCCC AGCAGCCCCT CCCACCTCTG CCAGCTCCGC CAGCAGCACA GACTGCAGGC 
CAGCTGCAGG TTCAAGCTCT AGCTCAGCAG CAACAGTCCC CTACAAAAGC TGTGCCGGCT 
TTGGGGAAAA GTCCTCCTCA CCACTCTGGA TTCCAGCAGT ATCAACAGGC AGATGCCTCC 
AAACAGCTGT GGAATCCCCC TCAGGTTCAA GGCCCATTAG GGAAAATCAT GCCTGTGAAA 
CAGCCCTACT ACCTTCAAAC CCAAGACCCC ATAAAACTGT TTGAGCCGTC GTTGCAACCT 
CCTGTAAGGC AGCAGCAGCC TCTAGAGAAA ACAATGAAGC CTTTCCCCAT GGAGCCATAT 
AACCATAACC CCTCAGAAGT CAAGATCCCG GAGTTCTACT GGGATTCTTC CTACAGCGCT 
GCTGATAACA GAGCTGTGAT GGCACAGCAA GCCAGTGTGG ACCGCAGGGG CAAACGGTCC 
CCGGGAGTCT TCCGTCCAGA GCAGGACCCT GTGCCCAGGA TGCCATTTGA GGACCCCAAA 
GGCTCCCCTC TGCTTCCTCC GGACCTGTTA AAGAGTCTGG CTGCCTTGGA GGAAGAGGAA 
GAGCTGATTT TTTCTAACCC TCCTGATCTT TACCCAGCTC TGCTGGGGCC TCTCGCCTCT 
CTTCCTGGAC GAAGCCTCTT TAAATCCTTA CTGGAGAAGC CCTCAGAGCT CATGTCACAT 
TCATCCTCTT TCCTGTCCCT CACCGGATTC TCCCTCAATC AGGAGCGATA CCCAAATAAC 
AGTGTGTTCA ATGAGGTATA CGGGAAGAAC CTGACAACCA GCTCCAAAGC AGAACTTAAT 
CCCTCAGTGG CTCCCCAGGA AACCTCACTG TACTCGCTTT TTGAAGGGAC TCCATGGTCT 
CCATCTCTTC CTGCCAGTTC AGATCATTCA ACACCAGCCA GCCAATCTCC TCATTCCTCC 
AACCCAAGCA GCCTGCCAAG CTCCCCTCCA ACACACAACC ACAATTCTGT CCCATTCTCC 
AATTTTGGAC CCATTGGGAC TCCAGATAAC AGGGATAGGA GAACTGCAGA CCGGTGGAAA 
ACTGATAAGC CAGCCATGGG TGGGTTTGGC ATCGATTATC TCTCAGCAAC ATCATCCTCT 
GAGAGCAGTT GGCATCAGGC CAGCACTCCA AGTGGCTCCT GGACAGGCCA TGGCCCATCC 
ATGGAGGATT CCTCTGCTGT CCTCATGGAA AGCCTAAAGT CTATCTGGTC CAGTTCCATG 
ATGCATCCTG GACCTTCCGC TTTGGAGCAG CTGTTAATGC AGCAGAAGCA GAAACAGCAG 
CGGGGCCAAG GCGCCATGAA CCCTCCACAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010811828.2
CDS	92..3604
Translation	MRNWMFLQIVIVGENVSFKSQMRTGNLKSEEHLKSSNIRQAEVLKADMTDSKLGPAEVWTSRQALQDLYQKMLVTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRANPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQTHTSAIVKPQSSSCSYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNLQKALSKALESRDEVKTKWGVSDFIKAFIKFHGHVYLSKSLEKLSPLREKLEEQFKRLLFQKAFNSQQLVHVTVINLFQLHHLRDFSNETEQHSYSQDEQLCWTQLLALFMSFLGILCKCPLQNKSQEESYNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPHEEDLSSTNATPLPEEFELQGFLALRPSFRNLDFSKGHQGITGDKEGQQRQIRQQRLISIGKWIADNQPRLIQCENEVGKLLFITEIPELILEDPSEAKENLILQDTTIIESLAADGNPGLKSVLSTGRNLSNNCDPGEKPVVTFKENMKPREVSRDQGRSFPPKEVKSQTELRKTPVSEARKTPVTQTPSQASNSQFIPIHHPGAFPPLPSRPGFPPPTYVIPPPVAFSMGSGYTFPAGVSVPGTFLQPTAHSPPGNQVQAGKQSHIPYSQQRPSGPGPVTQGPQQPQPPSQQPLPPLPAPPAAQTAGQLQVQALAQQQQSPTKAVPALGKSPPHHSGFQQYQQADASKQLWNPPQVQGPLGKIMPVKQPYYLQTQDPIKLFEPSLQPPVRQQQPLEKTMKPFPMEPYNHNPSEVKIPEFYWDSSYSAADNRAVMAQQASVDRRGKRSPGVFRPEQDPVPRMPFEDPKGSPLLPPDLLKSLAALEEEEELIFSNPPDLYPALLGPLASLPGRSLFKSLLEKPSELMSHSSSFLSLTGFSLNQERYPNNSVFNEVYGKNLTTSSKAELNPSVAPQETSLYSLFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSVPFSNFGPIGTPDNRDRRTADRWKTDKPAMGGFGIDYLSATSSSESSWHQASTPSGSWTGHGPSMEDSSAVLMESLKSIWSSSMMHPGPSALEQLLMQQKQKQQRGQGAMNPPH

Target ORF information:

RefSeq Version	XM_010813526.2
Organism	Bos taurus(cattle)
Definition	Bos taurus SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010813526.2

ORF Insert Sequence:

ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
CAGATGAGGA CCGGAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG 
GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCCGA AGTCTGGACA 
TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTTG TTACTGATTT GGAATACGCT 
TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCATGCCT TTAAGAATCA GATCACAACA 
CTACAAGGCC AGGCAAAGAA TCGAGCAAAC CCGAATCGGA GTGAAGTTCA GGCAAACCTT 
TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA 
GTGTTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AGTTGGGAAT TATCAGCAAT 
AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC 
CAGCACTGCC TCGTTCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA 
GAGTCCTACT ATAGGCATGC GGCTCAGCTT GTCCCCTCTA ATGGTCAGCC TTACAATCAG 
TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACTA CAATTTTCTA CTACTGCAGA 
AGCATTGCTG TGAAATTCCC TTTCCCAGCT GCCTCTACTA ATCTACAAAA AGCACTTTCT 
AAAGCACTGG AAAGCCGGGA TGAGGTGAAA ACCAAATGGG GTGTTTCTGA CTTCATCAAG 
GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT 
CTTCGAGAGA AGTTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAGAAAGC TTTCAACTCT 
CAGCAGTTAG TTCACGTCAC TGTCATTAAC CTGTTTCAAC TTCATCATCT TCGTGACTTT 
AGCAACGAAA CGGAGCAGCA TAGTTATAGC CAAGATGAGC AGCTGTGTTG GACACAGTTG 
CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTCCA GAACAAGTCT 
CAGGAGGAAT CCTACAATGC CTATCCCCTA CCTGCCGTCA AGGTGTCCAT GGACTGGCTG 
AGACTCAGAC CCAGGGTGTT TCAGGAAGCA GTGGTGGATG AAAGACAGTA CATTTGGCCG 
TGGCTAATTT CTCTTCTGAA CAGTTTCCAT CCCCATGAAG AAGATCTTTC AAGTACTAAT 
GCAACACCAC TTCCAGAGGA GTTTGAGTTA CAAGGATTCT TGGCTTTGAG ACCTTCTTTC 
AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GAGACAAAGA GGGTCAGCAA 
CGACAAATAC GACAGCAGCG TTTGATCTCT ATAGGCAAAT GGATTGCAGA TAATCAGCCA 
AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAG 
TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA CACAACCATC 
ATAGAGTCCC TGGCAGCTGA CGGGAACCCA GGACTGAAAT CAGTGCTATC TACAGGCCGG 
AATCTAAGCA ACAACTGCGA CCCAGGAGAG AAACCAGTGG TCACCTTCAA AGAGAACATG 
AAGCCACGAG AAGTGAGCAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTAAAATCT 
CAGACAGAGT TAAGAAAGAC TCCAGTGTCT GAAGCCAGGA AAACACCTGT AACTCAAACC 
CCAAGTCAAG CAAGTAACTC CCAGTTCATC CCCATTCATC ACCCTGGAGC CTTCCCTCCT 
CTTCCCAGCC GGCCAGGGTT CCCGCCCCCA ACATATGTTA TCCCCCCTCC TGTGGCATTT 
TCTATGGGCT CAGGTTACAC CTTCCCAGCT GGTGTTTCTG TCCCAGGAAC CTTTCTTCAG 
CCTACAGCTC ACTCTCCACC AGGAAACCAG GTGCAAGCTG GGAAACAGTC CCACATTCCT 
TACAGCCAGC AACGGCCCTC CGGACCAGGG CCAGTGACCC AGGGACCTCA GCAGCCTCAG 
CCACCTTCCC AGCAGCCCCT CCCACCTCTG CCAGCTCCGC CAGCAGCACA GACTGCAGGC 
CAGCTGCAGG TTCAAGCTCT AGCTCAGCAG CAACAGTCCC CTACAAAAGC TGTGCCGGCT 
TTGGGGAAAA GTCCTCCTCA CCACTCTGGA TTCCAGCAGT ATCAACAGGC AGATGCCTCC 
AAACAGCTGT GGAATCCCCC TCAGGTTCAA GGCCCATTAG GGAAAATCAT GCCTGTGAAA 
CAGCCCTACT ACCTTCAAAC CCAAGACCCC ATAAAACTGT TTGAGCCGTC GTTGCAACCT 
CCTGTAAGGC AGCAGCAGCC TCTAGAGAAA ACAATGAAGC CTTTCCCCAT GGAGCCATAT 
AACCATAACC CCTCAGAAGT CAAGATCCCG GAGTTCTACT GGGATTCTTC CTACAGCGCT 
GCTGATAACA GAGCTGTGAT GGCACAGCAA GCCAGTGTGG ACCGCAGGGG CAAACGGTCC 
CCGGGAGTCT TCCGTCCAGA GCAGGACCCT GTGCCCAGGA TGCCATTTGA GGACCCCAAA 
GGCTCCCCTC TGCTTCCTCC GGACCTGTTA AAGAGTCTGG CTGCCTTGGA GGAAGAGGAA 
GAGCTGATTT TTTCTAACCC TCCTGATCTT TACCCAGCTC TGCTGGGGCC TCTCGCCTCT 
CTTCCTGGAC GAAGCCTCTT TAAATCCTTA CTGGAGAAGC CCTCAGAGCT CATGTCACAT 
TCATCCTCTT TCCTGTCCCT CACCGGATTC TCCCTCAATC AGGAGCGATA CCCAAATAAC 
AGTGTGTTCA ATGAGGTATA CGGGAAGAAC CTGACAACCA GCTCCAAAGC AGAACTTAAT 
CCCTCAGTGG CTCCCCAGGA AACCTCACTG TACTCGCTTT TTGAAGGGAC TCCATGGTCT 
CCATCTCTTC CTGCCAGTTC AGATCATTCA ACACCAGCCA GCCAATCTCC TCATTCCTCC 
AACCCAAGCA GCCTGCCAAG CTCCCCTCCA ACACACAACC ACAATTCTGT CCCATTCTCC 
AATTTTGGAC CCATTGGGAC TCCAGATAAC AGGGATAGGA GAACTGCAGA CCGGTGGAAA 
ACTGATAAGC CAGCCATGGG TGGGTTTGGC ATCGATTATC TCTCAGCAAC ATCATCCTCT 
GAGAGCAGTT GGCATCAGGC CAGCACTCCA AGTGGCTCCT GGACAGGCCA TGGCCCATCC 
ATGGAGGATT CCTCTGCTGT CCTCATGGAA AGCCTAAAGT CTATCTGGTC CAGTTCCATG 
ATGCATCCTG GACCTTCCGC TTTGGAGCAG CTGTTAATGC AGCAGAAGCA GAAACAGCAG 
CGGGGCCAAG GCGCCATGAA CCCTCCACAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG7 ( XM_015475257.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015330743.1 Bos taurus SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa93046
Clone ID Related Accession (Same CDS sequence)	XM_015475257.1
Accession Version	XM_015475257.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3501bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein SMG7 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104440.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
CAGATGAGGA CCGGAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG 
GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCCGA AGTCTGGACA 
TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTTG TTACTGATTT GGAATACGCT 
TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCATGCCT TTAAGAATCA GATCACAACA 
CTACAAGGCC AGGCAAAGAA TCGAGCAAAC CCGAATCGGA GTGAAGTTCA GGCAAACCTT 
TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA 
GTGTTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AGTTGGGAAT TATCAGCAAT 
AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC 
CAGCACTGCC TCGTTCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA 
GAGTCCTACT ATAGGCATGC GGCTCAGCTT GTCCCCTCTA ATGGTCAGCC TTACAATCAG 
TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACTA CAATTTTCTA CTACTGCAGA 
AGCATTGCTG TGAAATTCCC TTTCCCAGCT GCCTCTACTA ATCTACAAAA AGCACTTTCT 
AAAGCACTGG AAAGCCGGGA TGAGGTGAAA ACCAAATGGG GTGTTTCTGA CTTCATCAAG 
GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT 
CTTCGAGAGA AGTTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAGAAAGC TTTCAACTCT 
CAGCAGTTAG TTCACGTCAC TGTCATTAAC CTGTTTCAAC TTCATCATCT TCGTGACTTT 
AGCAACGAAA CGGAGCAGCA TAGTTATAGC CAAGATGAGC AGCTGTGTTG GACACAGTTG 
CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTCCA GAACAAGTCT 
CAGGAGGAAT CCTACAATGC CTATCCCCTA CCTGCCGTCA AGGTGTCCAT GGACTGGCTG 
AGACTCAGAC CCAGGGTGTT TCAGGAAGCA GTGGTGGATG AAAGACAGTA CATTTGGCCG 
TGGCTAATTT CTCTTCTGAA CAGTTTCCAT CCCCATGAAG AAGATCTTTC AAGTACTAAT 
GCAACACCAC TTCCAGAGGA GTTTGAGTTA CAAGGATTCT TGGCTTTGAG ACCTTCTTTC 
AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GAGACAAAGA GGGTCAGCAA 
CGACAAATAC GACAGCAGCG TTTGATCTCT ATAGGCAAAT GGATTGCAGA TAATCAGCCA 
AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAG 
TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA CACAACCATC 
ATAGAGTCCC TGGCAGCTGA CGGGAACCCA GGACTGAAAT CAGTGCTATC TACAGGCCGG 
AATCTAAGCA ACAACTGCGA CCCAGGAGAG AAACCAGTGG TCACCTTCAA AGAGAACATG 
AAGCCACGAG AAGTGAGCAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTGAGAAGG 
GACTATAGCA AAGGAGTAGC TGTAACTGAG AATGATGGAA AGAAGGACAA CAACAAGAGG 
AAAACAGAAA CCAAGAAATG CACCTTAGAA AAGTTACAGG AAACAGGAAA GCAGAGTGTG 
GCAGTGCAGG TAAAATCTCA GACAGAGTTA AGAAAGACTC CAGTGTCTGA AGCCAGGAAA 
ACACCTGTAA CTCAAACCCC AAGTCAAGCA AGTAACTCCC AGTTCATCCC CATTCATCAC 
CCTGGAGCCT TCCCTCCTCT TCCCAGCCGG CCAGGGTTCC CGCCCCCAAC ATATGTTATC 
CCCCCTCCTG TGGCATTTTC TATGGGCTCA GGTTACACCT TCCCAGCTGG TGTTTCTGTC 
CCAGGAACCT TTCTTCAGCC TACAGCTCAC TCTCCACCAG GAAACCAGGT GCAAGCTGGG 
AAACAGTCCC ACATTCCTTA CAGCCAGCAA CGGCCCTCCG GACCAGGGCC AGTGACCCAG 
GGACCTCAGC AGCCTCAGCC ACCTTCCCAG CAGCCCCTCC CACCTCTGCC AGCTCCGCCA 
GCAGCACAGA CTGCAGGCCA GCTGCAGGTT CAAGCTCTAG CTCAGCAGCA ACAGTCCCCT 
ACAAAAGCTG TGCCGGCTTT GGGGAAAAGT CCTCCTCACC ACTCTGGATT CCAGCAGTAT 
CAACAGGCAG ATGCCTCCAA ACAGCTGTGG AATCCCCCTC AGGTTCAAGG CCCATTAGGG 
AAAATCATGC CTGTGAAACA GCCCTACTAC CTTCAAACCC AAGACCCCAT AAAACTGTTT 
GAGCCGTCGT TGCAACCTCC TGTAAGGCAG CAGCAGCCTC TAGAGAAAAC AATGAAGCCT 
TTCCCCATGG AGCCATATAA CCATAACCCC TCAGAAGTCA AGATCCCGGA GTTCTACTGG 
GATTCTTCCT ACAGCGCTGC TGATAACAGA GCTGTGATGG CACAGCAAGC CAGTGTGGAC 
CGCAGGGGCA AACGGTCCCC GGGAGTCTTC CGTCCAGAGC AGGACCCTGT GCCCAGGATG 
CCATTTGAGA AATCCTTACT GGAGAAGCCC TCAGAGCTCA TGTCACATTC ATCCTCTTTC 
CTGTCCCTCA CCGGATTCTC CCTCAATCAG GAGCGATACC CAAATAACAG TGTGTTCAAT 
GAGGTATACG GGAAGAACCT GACAACCAGC TCCAAAGCAG AACTTAATCC CTCAGTGGCT 
CCCCAGGAAA CCTCACTGTA CTCGCTTTTT GAAGGGACTC CATGGTCTCC ATCTCTTCCT 
GCCAGTTCAG ATCATTCAAC ACCAGCCAGC CAATCTCCTC ATTCCTCCAA CCCAAGCAGC 
CTGCCAAGCT CCCCTCCAAC ACACAACCAC AATTCTGTCC CATTCTCCAA TTTTGGACCC 
ATTGGGACTC CAGATAACAG GGATAGGAGA ACTGCAGACC GGTGGAAAAC TGATAAGCCA 
GCCATGGGTG GGTTTGGCAT CGATTATCTC TCAGCAACAT CATCCTCTGA GAGCAGTTGG 
CATCAGGCCA GCACTCCAAG TGGCTCCTGG ACAGGCCATG GCCCATCCAT GGAGGATTCC 
TCTGCTGTCC TCATGGAAAG CCTAAAGTCT ATCTGGTCCA GTTCCATGAT GCATCCTGGA 
CCTTCCGCTT TGGAGCAGCT GTTAATGCAG CAGAAGCAGA AACAGCAGCG GGGCCAAGGC 
GCCATGAACC CTCCACACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015330743.1
CDS	92..3592
Translation	MRNWMFLQIVIVGENVSFKSQMRTGNLKSEEHLKSSNIRQAEVLKADMTDSKLGPAEVWTSRQALQDLYQKMLVTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRANPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQTHTSAIVKPQSSSCSYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNLQKALSKALESRDEVKTKWGVSDFIKAFIKFHGHVYLSKSLEKLSPLREKLEEQFKRLLFQKAFNSQQLVHVTVINLFQLHHLRDFSNETEQHSYSQDEQLCWTQLLALFMSFLGILCKCPLQNKSQEESYNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPHEEDLSSTNATPLPEEFELQGFLALRPSFRNLDFSKGHQGITGDKEGQQRQIRQQRLISIGKWIADNQPRLIQCENEVGKLLFITEIPELILEDPSEAKENLILQDTTIIESLAADGNPGLKSVLSTGRNLSNNCDPGEKPVVTFKENMKPREVSRDQGRSFPPKEVRRDYSKGVAVTENDGKKDNNKRKTETKKCTLEKLQETGKQSVAVQVKSQTELRKTPVSEARKTPVTQTPSQASNSQFIPIHHPGAFPPLPSRPGFPPPTYVIPPPVAFSMGSGYTFPAGVSVPGTFLQPTAHSPPGNQVQAGKQSHIPYSQQRPSGPGPVTQGPQQPQPPSQQPLPPLPAPPAAQTAGQLQVQALAQQQQSPTKAVPALGKSPPHHSGFQQYQQADASKQLWNPPQVQGPLGKIMPVKQPYYLQTQDPIKLFEPSLQPPVRQQQPLEKTMKPFPMEPYNHNPSEVKIPEFYWDSSYSAADNRAVMAQQASVDRRGKRSPGVFRPEQDPVPRMPFEKSLLEKPSELMSHSSSFLSLTGFSLNQERYPNNSVFNEVYGKNLTTSSKAELNPSVAPQETSLYSLFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSVPFSNFGPIGTPDNRDRRTADRWKTDKPAMGGFGIDYLSATSSSESSWHQASTPSGSWTGHGPSMEDSSAVLMESLKSIWSSSMMHPGPSALEQLLMQQKQKQQRGQGAMNPPH

Target ORF information:

RefSeq Version	XM_015475257.1
Organism	Bos taurus(cattle)
Definition	Bos taurus SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015475257.1

ORF Insert Sequence:

ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
CAGATGAGGA CCGGAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG 
GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCCGA AGTCTGGACA 
TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTTG TTACTGATTT GGAATACGCT 
TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCATGCCT TTAAGAATCA GATCACAACA 
CTACAAGGCC AGGCAAAGAA TCGAGCAAAC CCGAATCGGA GTGAAGTTCA GGCAAACCTT 
TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA 
GTGTTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AGTTGGGAAT TATCAGCAAT 
AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC 
CAGCACTGCC TCGTTCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA 
GAGTCCTACT ATAGGCATGC GGCTCAGCTT GTCCCCTCTA ATGGTCAGCC TTACAATCAG 
TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACTA CAATTTTCTA CTACTGCAGA 
AGCATTGCTG TGAAATTCCC TTTCCCAGCT GCCTCTACTA ATCTACAAAA AGCACTTTCT 
AAAGCACTGG AAAGCCGGGA TGAGGTGAAA ACCAAATGGG GTGTTTCTGA CTTCATCAAG 
GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT 
CTTCGAGAGA AGTTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAGAAAGC TTTCAACTCT 
CAGCAGTTAG TTCACGTCAC TGTCATTAAC CTGTTTCAAC TTCATCATCT TCGTGACTTT 
AGCAACGAAA CGGAGCAGCA TAGTTATAGC CAAGATGAGC AGCTGTGTTG GACACAGTTG 
CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTCCA GAACAAGTCT 
CAGGAGGAAT CCTACAATGC CTATCCCCTA CCTGCCGTCA AGGTGTCCAT GGACTGGCTG 
AGACTCAGAC CCAGGGTGTT TCAGGAAGCA GTGGTGGATG AAAGACAGTA CATTTGGCCG 
TGGCTAATTT CTCTTCTGAA CAGTTTCCAT CCCCATGAAG AAGATCTTTC AAGTACTAAT 
GCAACACCAC TTCCAGAGGA GTTTGAGTTA CAAGGATTCT TGGCTTTGAG ACCTTCTTTC 
AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GAGACAAAGA GGGTCAGCAA 
CGACAAATAC GACAGCAGCG TTTGATCTCT ATAGGCAAAT GGATTGCAGA TAATCAGCCA 
AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAG 
TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA CACAACCATC 
ATAGAGTCCC TGGCAGCTGA CGGGAACCCA GGACTGAAAT CAGTGCTATC TACAGGCCGG 
AATCTAAGCA ACAACTGCGA CCCAGGAGAG AAACCAGTGG TCACCTTCAA AGAGAACATG 
AAGCCACGAG AAGTGAGCAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTGAGAAGG 
GACTATAGCA AAGGAGTAGC TGTAACTGAG AATGATGGAA AGAAGGACAA CAACAAGAGG 
AAAACAGAAA CCAAGAAATG CACCTTAGAA AAGTTACAGG AAACAGGAAA GCAGAGTGTG 
GCAGTGCAGG TAAAATCTCA GACAGAGTTA AGAAAGACTC CAGTGTCTGA AGCCAGGAAA 
ACACCTGTAA CTCAAACCCC AAGTCAAGCA AGTAACTCCC AGTTCATCCC CATTCATCAC 
CCTGGAGCCT TCCCTCCTCT TCCCAGCCGG CCAGGGTTCC CGCCCCCAAC ATATGTTATC 
CCCCCTCCTG TGGCATTTTC TATGGGCTCA GGTTACACCT TCCCAGCTGG TGTTTCTGTC 
CCAGGAACCT TTCTTCAGCC TACAGCTCAC TCTCCACCAG GAAACCAGGT GCAAGCTGGG 
AAACAGTCCC ACATTCCTTA CAGCCAGCAA CGGCCCTCCG GACCAGGGCC AGTGACCCAG 
GGACCTCAGC AGCCTCAGCC ACCTTCCCAG CAGCCCCTCC CACCTCTGCC AGCTCCGCCA 
GCAGCACAGA CTGCAGGCCA GCTGCAGGTT CAAGCTCTAG CTCAGCAGCA ACAGTCCCCT 
ACAAAAGCTG TGCCGGCTTT GGGGAAAAGT CCTCCTCACC ACTCTGGATT CCAGCAGTAT 
CAACAGGCAG ATGCCTCCAA ACAGCTGTGG AATCCCCCTC AGGTTCAAGG CCCATTAGGG 
AAAATCATGC CTGTGAAACA GCCCTACTAC CTTCAAACCC AAGACCCCAT AAAACTGTTT 
GAGCCGTCGT TGCAACCTCC TGTAAGGCAG CAGCAGCCTC TAGAGAAAAC AATGAAGCCT 
TTCCCCATGG AGCCATATAA CCATAACCCC TCAGAAGTCA AGATCCCGGA GTTCTACTGG 
GATTCTTCCT ACAGCGCTGC TGATAACAGA GCTGTGATGG CACAGCAAGC CAGTGTGGAC 
CGCAGGGGCA AACGGTCCCC GGGAGTCTTC CGTCCAGAGC AGGACCCTGT GCCCAGGATG 
CCATTTGAGA AATCCTTACT GGAGAAGCCC TCAGAGCTCA TGTCACATTC ATCCTCTTTC 
CTGTCCCTCA CCGGATTCTC CCTCAATCAG GAGCGATACC CAAATAACAG TGTGTTCAAT 
GAGGTATACG GGAAGAACCT GACAACCAGC TCCAAAGCAG AACTTAATCC CTCAGTGGCT 
CCCCAGGAAA CCTCACTGTA CTCGCTTTTT GAAGGGACTC CATGGTCTCC ATCTCTTCCT 
GCCAGTTCAG ATCATTCAAC ACCAGCCAGC CAATCTCCTC ATTCCTCCAA CCCAAGCAGC 
CTGCCAAGCT CCCCTCCAAC ACACAACCAC AATTCTGTCC CATTCTCCAA TTTTGGACCC 
ATTGGGACTC CAGATAACAG GGATAGGAGA ACTGCAGACC GGTGGAAAAC TGATAAGCCA 
GCCATGGGTG GGTTTGGCAT CGATTATCTC TCAGCAACAT CATCCTCTGA GAGCAGTTGG 
CATCAGGCCA GCACTCCAAG TGGCTCCTGG ACAGGCCATG GCCCATCCAT GGAGGATTCC 
TCTGCTGTCC TCATGGAAAG CCTAAAGTCT ATCTGGTCCA GTTCCATGAT GCATCCTGGA 
CCTTCCGCTT TGGAGCAGCT GTTAATGCAG CAGAAGCAGA AACAGCAGCG GGGCCAAGGC 
GCCATGAACC CTCCACACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG7 ( XM_015475258.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015330744.1 Bos taurus SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X5, mRNA.

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CloneID	OBa93047
Clone ID Related Accession (Same CDS sequence)	XM_015475258.1
Accession Version	XM_015475258.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3423bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein SMG7 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104440.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
CAGATGAGGA CCGGAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG 
GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCCGA AGTCTGGACA 
TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTTG TTACTGATTT GGAATACGCT 
TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCATGCCT TTAAGAATCA GATCACAACA 
CTACAAGGCC AGGCAAAGAA TCGAGCAAAC CCGAATCGGA GTGAAGTTCA GGCAAACCTT 
TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA 
GTGTTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AGTTGGGAAT TATCAGCAAT 
AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC 
CAGCACTGCC TCGTTCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA 
GAGTCCTACT ATAGGCATGC GGCTCAGCTT GTCCCCTCTA ATGGTCAGCC TTACAATCAG 
TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACTA CAATTTTCTA CTACTGCAGA 
AGCATTGCTG TGAAATTCCC TTTCCCAGCT GCCTCTACTA ATCTACAAAA AGCACTTTCT 
AAAGCACTGG AAAGCCGGGA TGAGGTGAAA ACCAAATGGG GTGTTTCTGA CTTCATCAAG 
GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT 
CTTCGAGAGA AGTTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAGAAAGC TTTCAACTCT 
CAGCAGTTAG TTCACGTCAC TGTCATTAAC CTGTTTCAAC TTCATCATCT TCGTGACTTT 
AGCAACGAAA CGGAGCAGCA TAGTTATAGC CAAGATGAGC AGCTGTGTTG GACACAGTTG 
CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTCCA GAACAAGTCT 
CAGGAGGAAT CCTACAATGC CTATCCCCTA CCTGCCGTCA AGGTGTCCAT GGACTGGCTG 
AGACTCAGAC CCAGGGTGTT TCAGGAAGCA GTGGTGGATG AAAGACAGTA CATTTGGCCG 
TGGCTAATTT CTCTTCTGAA CAGTTTCCAT CCCCATGAAG AAGATCTTTC AAGTACTAAT 
GCAACACCAC TTCCAGAGGA GTTTGAGTTA CAAGGATTCT TGGCTTTGAG ACCTTCTTTC 
AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GAGACAAAGA GGGTCAGCAA 
CGACAAATAC GACAGCAGCG TTTGATCTCT ATAGGCAAAT GGATTGCAGA TAATCAGCCA 
AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAG 
TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA CACAACCATC 
ATAGAGTCCC TGGCAGCTGA CGGGAACCCA GGACTGAAAT CAGTGCTATC TACAGGCCGG 
AATCTAAGCA ACAACTGCGA CCCAGGAGAG AAACCAGTGG TCACCTTCAA AGAGAACATG 
AAGCCACGAG AAGTGAGCAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTAAAATCT 
CAGACAGAGT TAAGAAAGAC TCCAGTGTCT GAAGCCAGGA AAACACCTGT AACTCAAACC 
CCAAGTCAAG CAAGTAACTC CCAGTTCATC CCCATTCATC ACCCTGGAGC CTTCCCTCCT 
CTTCCCAGCC GGCCAGGAAC CTTTCTTCAG CCTACAGCTC ACTCTCCACC AGGAAACCAG 
GTGCAAGCTG GGAAACAGTC CCACATTCCT TACAGCCAGC AACGGCCCTC CGGACCAGGG 
CCAGTGACCC AGGGACCTCA GCAGCCTCAG CCACCTTCCC AGCAGCCCCT CCCACCTCTG 
CCAGCTCCGC CAGCAGCACA GACTGCAGGC CAGCTGCAGG TTCAAGCTCT AGCTCAGCAG 
CAACAGTCCC CTACAAAAGC TGTGCCGGCT TTGGGGAAAA GTCCTCCTCA CCACTCTGGA 
TTCCAGCAGT ATCAACAGGC AGATGCCTCC AAACAGCTGT GGAATCCCCC TCAGGTTCAA 
GGCCCATTAG GGAAAATCAT GCCTGTGAAA CAGCCCTACT ACCTTCAAAC CCAAGACCCC 
ATAAAACTGT TTGAGCCGTC GTTGCAACCT CCTGTAAGGC AGCAGCAGCC TCTAGAGAAA 
ACAATGAAGC CTTTCCCCAT GGAGCCATAT AACCATAACC CCTCAGAAGT CAAGATCCCG 
GAGTTCTACT GGGATTCTTC CTACAGCGCT GCTGATAACA GAGCTGTGAT GGCACAGCAA 
GCCAGTGTGG ACCGCAGGGG CAAACGGTCC CCGGGAGTCT TCCGTCCAGA GCAGGACCCT 
GTGCCCAGGA TGCCATTTGA GGACCCCAAA GGCTCCCCTC TGCTTCCTCC GGACCTGTTA 
AAGAGTCTGG CTGCCTTGGA GGAAGAGGAA GAGCTGATTT TTTCTAACCC TCCTGATCTT 
TACCCAGCTC TGCTGGGGCC TCTCGCCTCT CTTCCTGGAC GAAGCCTCTT TAAATCCTTA 
CTGGAGAAGC CCTCAGAGCT CATGTCACAT TCATCCTCTT TCCTGTCCCT CACCGGATTC 
TCCCTCAATC AGGAGCGATA CCCAAATAAC AGTGTGTTCA ATGAGGTATA CGGGAAGAAC 
CTGACAACCA GCTCCAAAGC AGAACTTAAT CCCTCAGTGG CTCCCCAGGA AACCTCACTG 
TACTCGCTTT TTGAAGGGAC TCCATGGTCT CCATCTCTTC CTGCCAGTTC AGATCATTCA 
ACACCAGCCA GCCAATCTCC TCATTCCTCC AACCCAAGCA GCCTGCCAAG CTCCCCTCCA 
ACACACAACC ACAATTCTGT CCCATTCTCC AATTTTGGAC CCATTGGGAC TCCAGATAAC 
AGGGATAGGA GAACTGCAGA CCGGTGGAAA ACTGATAAGC CAGCCATGGG TGGGTTTGGC 
ATCGATTATC TCTCAGCAAC ATCATCCTCT GAGAGCAGTT GGCATCAGGC CAGCACTCCA 
AGTGGCTCCT GGACAGGCCA TGGCCCATCC ATGGAGGATT CCTCTGCTGT CCTCATGGAA 
AGCCTAAAGT CTATCTGGTC CAGTTCCATG ATGCATCCTG GACCTTCCGC TTTGGAGCAG 
CTGTTAATGC AGCAGAAGCA GAAACAGCAG CGGGGCCAAG GCGCCATGAA CCCTCCACAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015330744.1
CDS	92..3514
Translation	MRNWMFLQIVIVGENVSFKSQMRTGNLKSEEHLKSSNIRQAEVLKADMTDSKLGPAEVWTSRQALQDLYQKMLVTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRANPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQTHTSAIVKPQSSSCSYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNLQKALSKALESRDEVKTKWGVSDFIKAFIKFHGHVYLSKSLEKLSPLREKLEEQFKRLLFQKAFNSQQLVHVTVINLFQLHHLRDFSNETEQHSYSQDEQLCWTQLLALFMSFLGILCKCPLQNKSQEESYNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPHEEDLSSTNATPLPEEFELQGFLALRPSFRNLDFSKGHQGITGDKEGQQRQIRQQRLISIGKWIADNQPRLIQCENEVGKLLFITEIPELILEDPSEAKENLILQDTTIIESLAADGNPGLKSVLSTGRNLSNNCDPGEKPVVTFKENMKPREVSRDQGRSFPPKEVKSQTELRKTPVSEARKTPVTQTPSQASNSQFIPIHHPGAFPPLPSRPGTFLQPTAHSPPGNQVQAGKQSHIPYSQQRPSGPGPVTQGPQQPQPPSQQPLPPLPAPPAAQTAGQLQVQALAQQQQSPTKAVPALGKSPPHHSGFQQYQQADASKQLWNPPQVQGPLGKIMPVKQPYYLQTQDPIKLFEPSLQPPVRQQQPLEKTMKPFPMEPYNHNPSEVKIPEFYWDSSYSAADNRAVMAQQASVDRRGKRSPGVFRPEQDPVPRMPFEDPKGSPLLPPDLLKSLAALEEEEELIFSNPPDLYPALLGPLASLPGRSLFKSLLEKPSELMSHSSSFLSLTGFSLNQERYPNNSVFNEVYGKNLTTSSKAELNPSVAPQETSLYSLFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSVPFSNFGPIGTPDNRDRRTADRWKTDKPAMGGFGIDYLSATSSSESSWHQASTPSGSWTGHGPSMEDSSAVLMESLKSIWSSSMMHPGPSALEQLLMQQKQKQQRGQGAMNPPH

Target ORF information:

RefSeq Version	XM_015475258.1
Organism	Bos taurus(cattle)
Definition	Bos taurus SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015475258.1

ORF Insert Sequence:

ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
CAGATGAGGA CCGGAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG 
GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCCGA AGTCTGGACA 
TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTTG TTACTGATTT GGAATACGCT 
TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCATGCCT TTAAGAATCA GATCACAACA 
CTACAAGGCC AGGCAAAGAA TCGAGCAAAC CCGAATCGGA GTGAAGTTCA GGCAAACCTT 
TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA 
GTGTTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AGTTGGGAAT TATCAGCAAT 
AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC 
CAGCACTGCC TCGTTCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA 
GAGTCCTACT ATAGGCATGC GGCTCAGCTT GTCCCCTCTA ATGGTCAGCC TTACAATCAG 
TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACTA CAATTTTCTA CTACTGCAGA 
AGCATTGCTG TGAAATTCCC TTTCCCAGCT GCCTCTACTA ATCTACAAAA AGCACTTTCT 
AAAGCACTGG AAAGCCGGGA TGAGGTGAAA ACCAAATGGG GTGTTTCTGA CTTCATCAAG 
GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT 
CTTCGAGAGA AGTTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAGAAAGC TTTCAACTCT 
CAGCAGTTAG TTCACGTCAC TGTCATTAAC CTGTTTCAAC TTCATCATCT TCGTGACTTT 
AGCAACGAAA CGGAGCAGCA TAGTTATAGC CAAGATGAGC AGCTGTGTTG GACACAGTTG 
CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTCCA GAACAAGTCT 
CAGGAGGAAT CCTACAATGC CTATCCCCTA CCTGCCGTCA AGGTGTCCAT GGACTGGCTG 
AGACTCAGAC CCAGGGTGTT TCAGGAAGCA GTGGTGGATG AAAGACAGTA CATTTGGCCG 
TGGCTAATTT CTCTTCTGAA CAGTTTCCAT CCCCATGAAG AAGATCTTTC AAGTACTAAT 
GCAACACCAC TTCCAGAGGA GTTTGAGTTA CAAGGATTCT TGGCTTTGAG ACCTTCTTTC 
AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GAGACAAAGA GGGTCAGCAA 
CGACAAATAC GACAGCAGCG TTTGATCTCT ATAGGCAAAT GGATTGCAGA TAATCAGCCA 
AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAG 
TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA CACAACCATC 
ATAGAGTCCC TGGCAGCTGA CGGGAACCCA GGACTGAAAT CAGTGCTATC TACAGGCCGG 
AATCTAAGCA ACAACTGCGA CCCAGGAGAG AAACCAGTGG TCACCTTCAA AGAGAACATG 
AAGCCACGAG AAGTGAGCAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTAAAATCT 
CAGACAGAGT TAAGAAAGAC TCCAGTGTCT GAAGCCAGGA AAACACCTGT AACTCAAACC 
CCAAGTCAAG CAAGTAACTC CCAGTTCATC CCCATTCATC ACCCTGGAGC CTTCCCTCCT 
CTTCCCAGCC GGCCAGGAAC CTTTCTTCAG CCTACAGCTC ACTCTCCACC AGGAAACCAG 
GTGCAAGCTG GGAAACAGTC CCACATTCCT TACAGCCAGC AACGGCCCTC CGGACCAGGG 
CCAGTGACCC AGGGACCTCA GCAGCCTCAG CCACCTTCCC AGCAGCCCCT CCCACCTCTG 
CCAGCTCCGC CAGCAGCACA GACTGCAGGC CAGCTGCAGG TTCAAGCTCT AGCTCAGCAG 
CAACAGTCCC CTACAAAAGC TGTGCCGGCT TTGGGGAAAA GTCCTCCTCA CCACTCTGGA 
TTCCAGCAGT ATCAACAGGC AGATGCCTCC AAACAGCTGT GGAATCCCCC TCAGGTTCAA 
GGCCCATTAG GGAAAATCAT GCCTGTGAAA CAGCCCTACT ACCTTCAAAC CCAAGACCCC 
ATAAAACTGT TTGAGCCGTC GTTGCAACCT CCTGTAAGGC AGCAGCAGCC TCTAGAGAAA 
ACAATGAAGC CTTTCCCCAT GGAGCCATAT AACCATAACC CCTCAGAAGT CAAGATCCCG 
GAGTTCTACT GGGATTCTTC CTACAGCGCT GCTGATAACA GAGCTGTGAT GGCACAGCAA 
GCCAGTGTGG ACCGCAGGGG CAAACGGTCC CCGGGAGTCT TCCGTCCAGA GCAGGACCCT 
GTGCCCAGGA TGCCATTTGA GGACCCCAAA GGCTCCCCTC TGCTTCCTCC GGACCTGTTA 
AAGAGTCTGG CTGCCTTGGA GGAAGAGGAA GAGCTGATTT TTTCTAACCC TCCTGATCTT 
TACCCAGCTC TGCTGGGGCC TCTCGCCTCT CTTCCTGGAC GAAGCCTCTT TAAATCCTTA 
CTGGAGAAGC CCTCAGAGCT CATGTCACAT TCATCCTCTT TCCTGTCCCT CACCGGATTC 
TCCCTCAATC AGGAGCGATA CCCAAATAAC AGTGTGTTCA ATGAGGTATA CGGGAAGAAC 
CTGACAACCA GCTCCAAAGC AGAACTTAAT CCCTCAGTGG CTCCCCAGGA AACCTCACTG 
TACTCGCTTT TTGAAGGGAC TCCATGGTCT CCATCTCTTC CTGCCAGTTC AGATCATTCA 
ACACCAGCCA GCCAATCTCC TCATTCCTCC AACCCAAGCA GCCTGCCAAG CTCCCCTCCA 
ACACACAACC ACAATTCTGT CCCATTCTCC AATTTTGGAC CCATTGGGAC TCCAGATAAC 
AGGGATAGGA GAACTGCAGA CCGGTGGAAA ACTGATAAGC CAGCCATGGG TGGGTTTGGC 
ATCGATTATC TCTCAGCAAC ATCATCCTCT GAGAGCAGTT GGCATCAGGC CAGCACTCCA 
AGTGGCTCCT GGACAGGCCA TGGCCCATCC ATGGAGGATT CCTCTGCTGT CCTCATGGAA 
AGCCTAAAGT CTATCTGGTC CAGTTCCATG ATGCATCCTG GACCTTCCGC TTTGGAGCAG 
CTGTTAATGC AGCAGAAGCA GAAACAGCAG CGGGGCCAAG GCGCCATGAA CCCTCCACAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG7 ( XM_010813528.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010811830.2 Bos taurus SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X6, mRNA.

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CloneID	OBa93048
Clone ID Related Accession (Same CDS sequence)	XM_010813528.2
Accession Version	XM_010813528.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3363bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein SMG7 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104440.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010813528.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
CAGATGAGGA CCGGAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG 
GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCCGA AGTCTGGACA 
TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTTG TTACTGATTT GGAATACGCT 
TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCATGCCT TTAAGAATCA GATCACAACA 
CTACAAGGCC AGGCAAAGAA TCGAGCAAAC CCGAATCGGA GTGAAGTTCA GGCAAACCTT 
TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA 
GTGTTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AGTTGGGAAT TATCAGCAAT 
AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC 
CAGCACTGCC TCGTTCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA 
GAGTCCTACT ATAGGCATGC GGCTCAGCTT GTCCCCTCTA ATGGTCAGCC TTACAATCAG 
TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACTA CAATTTTCTA CTACTGCAGA 
AGCATTGCTG TGAAATTCCC TTTCCCAGCT GCCTCTACTA ATCTACAAAA AGCACTTTCT 
AAAGCACTGG AAAGCCGGGA TGAGGTGAAA ACCAAATGGG GTGTTTCTGA CTTCATCAAG 
GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT 
CTTCGAGAGA AGTTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAGAAAGC TTTCAACTCT 
CAGCAGTTAG TTCACGTCAC TGTCATTAAC CTGTTTCAAC TTCATCATCT TCGTGACTTT 
AGCAACGAAA CGGAGCAGCA TAGTTATAGC CAAGATGAGC AGCTGTGTTG GACACAGTTG 
CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTCCA GAACAAGTCT 
CAGGAGGAAT CCTACAATGC CTATCCCCTA CCTGCCGTCA AGGTGTCCAT GGACTGGCTG 
AGACTCAGAC CCAGGGTGTT TCAGGAAGCA GTGGTGGATG AAAGACAGTA CATTTGGCCG 
TGGCTAATTT CTCTTCTGAA CAGTTTCCAT CCCCATGAAG AAGATCTTTC AAGTACTAAT 
GCAACACCAC TTCCAGAGGA GTTTGAGTTA CAAGGATTCT TGGCTTTGAG ACCTTCTTTC 
AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GAGACAAAGA GGGTCAGCAA 
CGACAAATAC GACAGCAGCG TTTGATCTCT ATAGGCAAAT GGATTGCAGA TAATCAGCCA 
AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAG 
TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA CACAACCATC 
ATAGAGTCCC TGGCAGCTGA CGGGAACCCA GGACTGAAAT CAGTGCTATC TACAGGCCGG 
AATCTAAGCA ACAACTGCGA CCCAGGAGAG AAACCAGTGG TCACCTTCAA AGAGAACATG 
AAGCCACGAG AAGTGAGCAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTAAAATCT 
CAGACAGAGT TAAGAAAGAC TCCAGTGTCT GAAGCCAGGA AAACACCTGT AACTCAAACC 
CCAAGTCAAG CAAGTAACTC CCAGTTCATC CCCATTCATC ACCCTGGAGC CTTCCCTCCT 
CTTCCCAGCC GGCCAGGGTT CCCGCCCCCA ACATATGTTA TCCCCCCTCC TGTGGCATTT 
TCTATGGGCT CAGGTTACAC CTTCCCAGCT GGTGTTTCTG TCCCAGGAAC CTTTCTTCAG 
CCTACAGCTC ACTCTCCACC AGGAAACCAG GTGCAAGCTG GGAAACAGTC CCACATTCCT 
TACAGCCAGC AACGGCCCTC CGGACCAGGG CCAGTGACCC AGGGACCTCA GCAGCCTCAG 
CCACCTTCCC AGCAGCCCCT CCCACCTCTG CCAGCTCCGC CAGCAGCACA GACTGCAGGC 
CAGCTGCAGG TTCAAGCTCT AGCTCAGCAG CAACAGTCCC CTACAAAAGC TGTGCCGGCT 
TTGGGGAAAA GTCCTCCTCA CCACTCTGGA TTCCAGCAGT ATCAACAGGC AGATGCCTCC 
AAACAGCTGT GGAATCCCCC TCAGGTTCAA GGCCCATTAG GGAAAATCAT GCCTGTGAAA 
CAGCCCTACT ACCTTCAAAC CCAAGACCCC ATAAAACTGT TTGAGCCGTC GTTGCAACCT 
CCTGTAAGGC AGCAGCAGCC TCTAGAGAAA ACAATGAAGC CTTTCCCCAT GGAGCCATAT 
AACCATAACC CCTCAGAAGT CAAGATCCCG GAGTTCTACT GGGATTCTTC CTACAGCGCT 
GCTGATAACA GAGCTGTGAT GGCACAGCAA GCCAGTGTGG ACCGCAGGGG CAAACGGTCC 
CCGGGAGTCT TCCGTCCAGA GCAGGACCCT GTGCCCAGGA TGCCATTTGA GAAATCCTTA 
CTGGAGAAGC CCTCAGAGCT CATGTCACAT TCATCCTCTT TCCTGTCCCT CACCGGATTC 
TCCCTCAATC AGGAGCGATA CCCAAATAAC AGTGTGTTCA ATGAGGTATA CGGGAAGAAC 
CTGACAACCA GCTCCAAAGC AGAACTTAAT CCCTCAGTGG CTCCCCAGGA AACCTCACTG 
TACTCGCTTT TTGAAGGGAC TCCATGGTCT CCATCTCTTC CTGCCAGTTC AGATCATTCA 
ACACCAGCCA GCCAATCTCC TCATTCCTCC AACCCAAGCA GCCTGCCAAG CTCCCCTCCA 
ACACACAACC ACAATTCTGT CCCATTCTCC AATTTTGGAC CCATTGGGAC TCCAGATAAC 
AGGGATAGGA GAACTGCAGA CCGGTGGAAA ACTGATAAGC CAGCCATGGG TGGGTTTGGC 
ATCGATTATC TCTCAGCAAC ATCATCCTCT GAGAGCAGTT GGCATCAGGC CAGCACTCCA 
AGTGGCTCCT GGACAGGCCA TGGCCCATCC ATGGAGGATT CCTCTGCTGT CCTCATGGAA 
AGCCTAAAGT CTATCTGGTC CAGTTCCATG ATGCATCCTG GACCTTCCGC TTTGGAGCAG 
CTGTTAATGC AGCAGAAGCA GAAACAGCAG CGGGGCCAAG GCGCCATGAA CCCTCCACAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010811830.2
CDS	92..3454
Translation	MRNWMFLQIVIVGENVSFKSQMRTGNLKSEEHLKSSNIRQAEVLKADMTDSKLGPAEVWTSRQALQDLYQKMLVTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRANPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQTHTSAIVKPQSSSCSYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNLQKALSKALESRDEVKTKWGVSDFIKAFIKFHGHVYLSKSLEKLSPLREKLEEQFKRLLFQKAFNSQQLVHVTVINLFQLHHLRDFSNETEQHSYSQDEQLCWTQLLALFMSFLGILCKCPLQNKSQEESYNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPHEEDLSSTNATPLPEEFELQGFLALRPSFRNLDFSKGHQGITGDKEGQQRQIRQQRLISIGKWIADNQPRLIQCENEVGKLLFITEIPELILEDPSEAKENLILQDTTIIESLAADGNPGLKSVLSTGRNLSNNCDPGEKPVVTFKENMKPREVSRDQGRSFPPKEVKSQTELRKTPVSEARKTPVTQTPSQASNSQFIPIHHPGAFPPLPSRPGFPPPTYVIPPPVAFSMGSGYTFPAGVSVPGTFLQPTAHSPPGNQVQAGKQSHIPYSQQRPSGPGPVTQGPQQPQPPSQQPLPPLPAPPAAQTAGQLQVQALAQQQQSPTKAVPALGKSPPHHSGFQQYQQADASKQLWNPPQVQGPLGKIMPVKQPYYLQTQDPIKLFEPSLQPPVRQQQPLEKTMKPFPMEPYNHNPSEVKIPEFYWDSSYSAADNRAVMAQQASVDRRGKRSPGVFRPEQDPVPRMPFEKSLLEKPSELMSHSSSFLSLTGFSLNQERYPNNSVFNEVYGKNLTTSSKAELNPSVAPQETSLYSLFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSVPFSNFGPIGTPDNRDRRTADRWKTDKPAMGGFGIDYLSATSSSESSWHQASTPSGSWTGHGPSMEDSSAVLMESLKSIWSSSMMHPGPSALEQLLMQQKQKQQRGQGAMNPPH

Target ORF information:

RefSeq Version	XM_010813528.2
Organism	Bos taurus(cattle)
Definition	Bos taurus SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010813528.2

ORF Insert Sequence:

ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
CAGATGAGGA CCGGAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG 
GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCCGA AGTCTGGACA 
TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTTG TTACTGATTT GGAATACGCT 
TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCATGCCT TTAAGAATCA GATCACAACA 
CTACAAGGCC AGGCAAAGAA TCGAGCAAAC CCGAATCGGA GTGAAGTTCA GGCAAACCTT 
TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA 
GTGTTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AGTTGGGAAT TATCAGCAAT 
AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC 
CAGCACTGCC TCGTTCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA 
GAGTCCTACT ATAGGCATGC GGCTCAGCTT GTCCCCTCTA ATGGTCAGCC TTACAATCAG 
TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACTA CAATTTTCTA CTACTGCAGA 
AGCATTGCTG TGAAATTCCC TTTCCCAGCT GCCTCTACTA ATCTACAAAA AGCACTTTCT 
AAAGCACTGG AAAGCCGGGA TGAGGTGAAA ACCAAATGGG GTGTTTCTGA CTTCATCAAG 
GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT 
CTTCGAGAGA AGTTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAGAAAGC TTTCAACTCT 
CAGCAGTTAG TTCACGTCAC TGTCATTAAC CTGTTTCAAC TTCATCATCT TCGTGACTTT 
AGCAACGAAA CGGAGCAGCA TAGTTATAGC CAAGATGAGC AGCTGTGTTG GACACAGTTG 
CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTCCA GAACAAGTCT 
CAGGAGGAAT CCTACAATGC CTATCCCCTA CCTGCCGTCA AGGTGTCCAT GGACTGGCTG 
AGACTCAGAC CCAGGGTGTT TCAGGAAGCA GTGGTGGATG AAAGACAGTA CATTTGGCCG 
TGGCTAATTT CTCTTCTGAA CAGTTTCCAT CCCCATGAAG AAGATCTTTC AAGTACTAAT 
GCAACACCAC TTCCAGAGGA GTTTGAGTTA CAAGGATTCT TGGCTTTGAG ACCTTCTTTC 
AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GAGACAAAGA GGGTCAGCAA 
CGACAAATAC GACAGCAGCG TTTGATCTCT ATAGGCAAAT GGATTGCAGA TAATCAGCCA 
AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAG 
TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA CACAACCATC 
ATAGAGTCCC TGGCAGCTGA CGGGAACCCA GGACTGAAAT CAGTGCTATC TACAGGCCGG 
AATCTAAGCA ACAACTGCGA CCCAGGAGAG AAACCAGTGG TCACCTTCAA AGAGAACATG 
AAGCCACGAG AAGTGAGCAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTAAAATCT 
CAGACAGAGT TAAGAAAGAC TCCAGTGTCT GAAGCCAGGA AAACACCTGT AACTCAAACC 
CCAAGTCAAG CAAGTAACTC CCAGTTCATC CCCATTCATC ACCCTGGAGC CTTCCCTCCT 
CTTCCCAGCC GGCCAGGGTT CCCGCCCCCA ACATATGTTA TCCCCCCTCC TGTGGCATTT 
TCTATGGGCT CAGGTTACAC CTTCCCAGCT GGTGTTTCTG TCCCAGGAAC CTTTCTTCAG 
CCTACAGCTC ACTCTCCACC AGGAAACCAG GTGCAAGCTG GGAAACAGTC CCACATTCCT 
TACAGCCAGC AACGGCCCTC CGGACCAGGG CCAGTGACCC AGGGACCTCA GCAGCCTCAG 
CCACCTTCCC AGCAGCCCCT CCCACCTCTG CCAGCTCCGC CAGCAGCACA GACTGCAGGC 
CAGCTGCAGG TTCAAGCTCT AGCTCAGCAG CAACAGTCCC CTACAAAAGC TGTGCCGGCT 
TTGGGGAAAA GTCCTCCTCA CCACTCTGGA TTCCAGCAGT ATCAACAGGC AGATGCCTCC 
AAACAGCTGT GGAATCCCCC TCAGGTTCAA GGCCCATTAG GGAAAATCAT GCCTGTGAAA 
CAGCCCTACT ACCTTCAAAC CCAAGACCCC ATAAAACTGT TTGAGCCGTC GTTGCAACCT 
CCTGTAAGGC AGCAGCAGCC TCTAGAGAAA ACAATGAAGC CTTTCCCCAT GGAGCCATAT 
AACCATAACC CCTCAGAAGT CAAGATCCCG GAGTTCTACT GGGATTCTTC CTACAGCGCT 
GCTGATAACA GAGCTGTGAT GGCACAGCAA GCCAGTGTGG ACCGCAGGGG CAAACGGTCC 
CCGGGAGTCT TCCGTCCAGA GCAGGACCCT GTGCCCAGGA TGCCATTTGA GAAATCCTTA 
CTGGAGAAGC CCTCAGAGCT CATGTCACAT TCATCCTCTT TCCTGTCCCT CACCGGATTC 
TCCCTCAATC AGGAGCGATA CCCAAATAAC AGTGTGTTCA ATGAGGTATA CGGGAAGAAC 
CTGACAACCA GCTCCAAAGC AGAACTTAAT CCCTCAGTGG CTCCCCAGGA AACCTCACTG 
TACTCGCTTT TTGAAGGGAC TCCATGGTCT CCATCTCTTC CTGCCAGTTC AGATCATTCA 
ACACCAGCCA GCCAATCTCC TCATTCCTCC AACCCAAGCA GCCTGCCAAG CTCCCCTCCA 
ACACACAACC ACAATTCTGT CCCATTCTCC AATTTTGGAC CCATTGGGAC TCCAGATAAC 
AGGGATAGGA GAACTGCAGA CCGGTGGAAA ACTGATAAGC CAGCCATGGG TGGGTTTGGC 
ATCGATTATC TCTCAGCAAC ATCATCCTCT GAGAGCAGTT GGCATCAGGC CAGCACTCCA 
AGTGGCTCCT GGACAGGCCA TGGCCCATCC ATGGAGGATT CCTCTGCTGT CCTCATGGAA 
AGCCTAAAGT CTATCTGGTC CAGTTCCATG ATGCATCCTG GACCTTCCGC TTTGGAGCAG 
CTGTTAATGC AGCAGAAGCA GAAACAGCAG CGGGGCCAAG GCGCCATGAA CCCTCCACAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG7 ( NM_001193205.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001180134.2 Bos taurus SMG7, nonsense mediated mRNA decay factor (SMG7), mRNA.

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CloneID	OBa39141
Clone ID Related Accession (Same CDS sequence)	NM_001193205.2
Accession Version	NM_001193205.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3414bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-22
Organism	Bos taurus(cattle)
Product	protein SMG7
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DAAA02043572.1, DAAA02043573.1 and DAAA02043574.1. On Apr 16, 2011 this sequence version replaced NM_001193205.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN02403640, SAMN02403648 [ECO:0000348] ##Evidence-Data-END##

ATGAGCCTGC AGAGCGCGCA GTATCTCCGG CAGGCAGAAG TCCTGAAGGC TGACATGACA 
GATTCTAAGC TGGGTCCAGC CGAAGTCTGG ACATCCAGGC AGGCTCTGCA GGACCTGTAC 
CAGAAAATGC TTGTTACTGA TTTGGAATAC GCTTTAGACA AGAAAGTAGA ACAGGATCTC 
TGGAATCATG CCTTTAAGAA TCAGATCACA ACACTACAAG GCCAGGCAAA GAATCGAGCA 
AACCCGAATC GGAGTGAAGT TCAGGCAAAC CTTTCTCTGT TCCTAGAGGC AGCTAGTGGC 
TTCTATACTC AGTTATTACA AGAACTGTGT ACAGTGTTTA ATGTAGATTT ACCATGCCGT 
GTGAAGTCTT CCCAGTTGGG AATTATCAGC AATAAACAGA CGCATACCAG CGCCATAGTG 
AAGCCACAGT CTAGCTCCTG TTCCTATATC TGCCAGCACT GCCTCGTTCA CCTTGGAGAC 
ATTGCTCGAT ACAGAAACCA GACCAGCCAG GCAGAGTCCT ACTATAGGCA TGCGGCTCAG 
CTTGTCCCCT CTAATGGTCA GCCTTACAAT CAGTTGGCTA TCTTAGCTTC TTCCAAAGGA 
GACCATCTGA CTACAATTTT CTACTACTGC AGAAGCATTG CTGTGAAATT CCCTTTCCCA 
GCTGCCTCTA CTAATCTACA AAAAGCACTT TCTAAAGCAC TGGAAAGCCG GGATGAGGTG 
AAAACCAAAT GGGGTGTTTC TGACTTCATC AAGGCCTTTA TTAAATTCCA CGGTCATGTG 
TACCTGAGTA AGAGCTTGGA AAAGTTGAGC CCTCTTCGAG AGAAGTTGGA AGAACAGTTT 
AAGAGGCTGC TATTCCAGAA AGCTTTCAAC TCTCAGCAGT TAGTTCACGT CACTGTCATT 
AACCTGTTTC AACTTCATCA TCTTCGTGAC TTTAGCAACG AAACGGAGCA GCATAGTTAT 
AGCCAAGATG AGCAGCTGTG TTGGACACAG TTGCTGGCCC TCTTTATGTC TTTTCTTGGC 
ATCCTGTGCA AGTGTCCTCT CCAGAACAAG TCTCAGGAGG AATCCTACAA TGCCTATCCC 
CTACCTGCCG TCAAGGTGTC CATGGACTGG CTGAGACTCA GACCCAGGGT GTTTCAGGAA 
GCAGTGGTGG ATGAAAGACA GTACATTTGG CCGTGGCTAA TTTCTCTTCT GAACAGTTTC 
CATCCCCATG AAGAAGATCT TTCAAGTACT AATGCAACAC CACTTCCAGA GGAGTTTGAG 
TTACAAGGAT TCTTGGCTTT GAGACCTTCT TTCAGGAACT TGGATTTTTC CAAAGGTCAC 
CAGGGTATTA CAGGAGACAA AGAGGGTCAG CAACGACAAA TACGACAGCA GCGTTTGATC 
TCTATAGGCA AATGGATTGC AGATAATCAG CCAAGGCTGA TTCAGTGTGA AAATGAGGTA 
GGGAAATTGT TGTTTATCAC AGAAATCCCA GAGTTAATAC TGGAAGACCC CAGTGAAGCC 
AAAGAGAACC TCATTCTGCA AGACACAACC ATCATAGAGT CCCTGGCAGC TGACGGGAAC 
CCAGGACTGA AATCAGTGCT ATCTACAGGC CGGAATCTAA GCAACAACTG CGACCCAGGA 
GAGAAACCAG TGGTCACCTT CAAAGAGAAC ATGAAGCCAC GAGAAGTGAG CAGAGACCAA 
GGAAGAAGTT TTCCTCCCAA AGAGGTGAGA AGGGACTATA GCAAAGGAGT AGCTGTAACT 
GAGAATGATG GAAAGAAGGA CAACAACAAG AGGAAAACAG AAACCAAGAA ATGCACCTTA 
GAAAAGTTAC AGGAAACAGG AAAGCAGAGT GTGGCAGTGC AGGTAAAATC TCAGACAGAG 
TTAAGAAAGA CTCCAGTGTC TGAAGCCAGG AAAACACCTG TAACTCAAAC CCCAAGTCAA 
GCAAGTAACT CCCAGTTCAT CCCCATTCAT CACCCTGGAG CCTTCCCTCC TCTTCCCAGC 
CGGCCAGGGT TCCCGCCCCC AACATATGTT ATCCCCCCTC CTGTGGCATT TTCTATGGGC 
TCAGGTTACA CCTTCCCAGC TGGTGTTTCT GTCCCAGGAA CCTTTCTTCA GCCTACAGCT 
CACTCTCCAC CAGGAAACCA GGTGCAAGCT GGGAAACAGT CCCACATTCC TTACAGCCAG 
CAACGGCCCT CCGGACCAGG GCCAGTGACC CAGGGACCTC AGCAGCCTCA GCCACCTTCC 
CAGCAGCCCC TCCCACCTCT GCCAGCTCCG CCAGCAGCAC AGACTGCAGG CCAGCTGCAG 
GTTCAAGCTC TAGCTCAGCA GCAACAGTCC CCTACAAAAG CTGTGCCGGC TTTGGGGAAA 
AGTCCTCCTC ACCACTCTGG ATTCCAGCAG TATCAACAGG CAGATGCCTC CAAACAGCTG 
TGGAATCCCC CTCAGGTTCA AGGCCCATTA GGGAAAATCA TGCCTGTGAA ACAGCCCTAC 
TACCTTCAAA CCCAAGACCC CATAAAACTG TTTGAGCCGT CGTTGCAACC TCCTGTAAGG 
CAGCAGCAGC CTCTAGAGAA AACAATGAAG CCTTTCCCCA TGGAGCCATA TAACCATAAC 
CCCTCAGAAG TCAAGATCCC GGAGTTCTAC TGGGATTCTT CCTACAGCGC TGCTGATAAC 
AGAGCTGTGA TGGCACAGCA AGCCAGTGTG GACCGCAGGG GCAAACGGTC CCCGGGAGTC 
TTCCGTCCAG AGCAGGACCC TGTGCCCAGG ATGCCATTTG AGAAATCCTT ACTGGAGAAG 
CCCTCAGAGC TCATGTCACA TTCATCCTCT TTCCTGTCCC TCACCGGATT CTCCCTCAAT 
CAGGAGCGAT ACCCAAATAA CAGTGTGTTC AATGAGGTAT ACGGGAAGAA CCTGACAACC 
AGCTCCAAAG CAGAACTTAA TCCCTCAGTG GCTCCCCAGG AAACCTCACT GTACTCGCTT 
TTTGAAGGGA CTCCATGGTC TCCATCTCTT CCTGCCAGTT CAGATCATTC AACACCAGCC 
AGCCAATCTC CTCATTCCTC CAACCCAAGC AGCCTGCCAA GCTCCCCTCC AACACACAAC 
CACAATTCTG TCCCATTCTC CAATTTTGGA CCCATTGGGA CTCCAGATAA CAGGGATAGG 
AGAACTGCAG ACCGGTGGAA AACTGATAAG CCAGCCATGG GTGGGTTTGG CATCGATTAT 
CTCTCAGCAA CATCATCCTC TGAGAGCAGT TGGCATCAGG CCAGCACTCC AAGTGGCTCC 
TGGACAGGCC ATGGCCCATC CATGGAGGAT TCCTCTGCTG TCCTCATGGA AAGCCTAAAG 
TCTATCTGGT CCAGTTCCAT GATGCATCCT GGACCTTCCG CTTTGGAGCA GCTGTTAATG 
CAGCAGAAGC AGAAACAGCA GCGGGGCCAA GGCGCCATGA ACCCTCCACA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001180134.2
CDS	154..3567
Translation	MSLQSAQYLRQAEVLKADMTDSKLGPAEVWTSRQALQDLYQKMLVTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRANPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQTHTSAIVKPQSSSCSYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNLQKALSKALESRDEVKTKWGVSDFIKAFIKFHGHVYLSKSLEKLSPLREKLEEQFKRLLFQKAFNSQQLVHVTVINLFQLHHLRDFSNETEQHSYSQDEQLCWTQLLALFMSFLGILCKCPLQNKSQEESYNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPHEEDLSSTNATPLPEEFELQGFLALRPSFRNLDFSKGHQGITGDKEGQQRQIRQQRLISIGKWIADNQPRLIQCENEVGKLLFITEIPELILEDPSEAKENLILQDTTIIESLAADGNPGLKSVLSTGRNLSNNCDPGEKPVVTFKENMKPREVSRDQGRSFPPKEVRRDYSKGVAVTENDGKKDNNKRKTETKKCTLEKLQETGKQSVAVQVKSQTELRKTPVSEARKTPVTQTPSQASNSQFIPIHHPGAFPPLPSRPGFPPPTYVIPPPVAFSMGSGYTFPAGVSVPGTFLQPTAHSPPGNQVQAGKQSHIPYSQQRPSGPGPVTQGPQQPQPPSQQPLPPLPAPPAAQTAGQLQVQALAQQQQSPTKAVPALGKSPPHHSGFQQYQQADASKQLWNPPQVQGPLGKIMPVKQPYYLQTQDPIKLFEPSLQPPVRQQQPLEKTMKPFPMEPYNHNPSEVKIPEFYWDSSYSAADNRAVMAQQASVDRRGKRSPGVFRPEQDPVPRMPFEKSLLEKPSELMSHSSSFLSLTGFSLNQERYPNNSVFNEVYGKNLTTSSKAELNPSVAPQETSLYSLFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSVPFSNFGPIGTPDNRDRRTADRWKTDKPAMGGFGIDYLSATSSSESSWHQASTPSGSWTGHGPSMEDSSAVLMESLKSIWSSSMMHPGPSALEQLLMQQKQKQQRGQGAMNPPH

Target ORF information:

RefSeq Version	NM_001193205.2
Organism	Bos taurus(cattle)
Definition	Bos taurus SMG7, nonsense mediated mRNA decay factor (SMG7), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001193205.2

ORF Insert Sequence:

ATGAGCCTGC AGAGCGCGCA GTATCTCCGG CAGGCAGAAG TCCTGAAGGC TGACATGACA 
GATTCTAAGC TGGGTCCAGC CGAAGTCTGG ACATCCAGGC AGGCTCTGCA GGACCTGTAC 
CAGAAAATGC TTGTTACTGA TTTGGAATAC GCTTTAGACA AGAAAGTAGA ACAGGATCTC 
TGGAATCATG CCTTTAAGAA TCAGATCACA ACACTACAAG GCCAGGCAAA GAATCGAGCA 
AACCCGAATC GGAGTGAAGT TCAGGCAAAC CTTTCTCTGT TCCTAGAGGC AGCTAGTGGC 
TTCTATACTC AGTTATTACA AGAACTGTGT ACAGTGTTTA ATGTAGATTT ACCATGCCGT 
GTGAAGTCTT CCCAGTTGGG AATTATCAGC AATAAACAGA CGCATACCAG CGCCATAGTG 
AAGCCACAGT CTAGCTCCTG TTCCTATATC TGCCAGCACT GCCTCGTTCA CCTTGGAGAC 
ATTGCTCGAT ACAGAAACCA GACCAGCCAG GCAGAGTCCT ACTATAGGCA TGCGGCTCAG 
CTTGTCCCCT CTAATGGTCA GCCTTACAAT CAGTTGGCTA TCTTAGCTTC TTCCAAAGGA 
GACCATCTGA CTACAATTTT CTACTACTGC AGAAGCATTG CTGTGAAATT CCCTTTCCCA 
GCTGCCTCTA CTAATCTACA AAAAGCACTT TCTAAAGCAC TGGAAAGCCG GGATGAGGTG 
AAAACCAAAT GGGGTGTTTC TGACTTCATC AAGGCCTTTA TTAAATTCCA CGGTCATGTG 
TACCTGAGTA AGAGCTTGGA AAAGTTGAGC CCTCTTCGAG AGAAGTTGGA AGAACAGTTT 
AAGAGGCTGC TATTCCAGAA AGCTTTCAAC TCTCAGCAGT TAGTTCACGT CACTGTCATT 
AACCTGTTTC AACTTCATCA TCTTCGTGAC TTTAGCAACG AAACGGAGCA GCATAGTTAT 
AGCCAAGATG AGCAGCTGTG TTGGACACAG TTGCTGGCCC TCTTTATGTC TTTTCTTGGC 
ATCCTGTGCA AGTGTCCTCT CCAGAACAAG TCTCAGGAGG AATCCTACAA TGCCTATCCC 
CTACCTGCCG TCAAGGTGTC CATGGACTGG CTGAGACTCA GACCCAGGGT GTTTCAGGAA 
GCAGTGGTGG ATGAAAGACA GTACATTTGG CCGTGGCTAA TTTCTCTTCT GAACAGTTTC 
CATCCCCATG AAGAAGATCT TTCAAGTACT AATGCAACAC CACTTCCAGA GGAGTTTGAG 
TTACAAGGAT TCTTGGCTTT GAGACCTTCT TTCAGGAACT TGGATTTTTC CAAAGGTCAC 
CAGGGTATTA CAGGAGACAA AGAGGGTCAG CAACGACAAA TACGACAGCA GCGTTTGATC 
TCTATAGGCA AATGGATTGC AGATAATCAG CCAAGGCTGA TTCAGTGTGA AAATGAGGTA 
GGGAAATTGT TGTTTATCAC AGAAATCCCA GAGTTAATAC TGGAAGACCC CAGTGAAGCC 
AAAGAGAACC TCATTCTGCA AGACACAACC ATCATAGAGT CCCTGGCAGC TGACGGGAAC 
CCAGGACTGA AATCAGTGCT ATCTACAGGC CGGAATCTAA GCAACAACTG CGACCCAGGA 
GAGAAACCAG TGGTCACCTT CAAAGAGAAC ATGAAGCCAC GAGAAGTGAG CAGAGACCAA 
GGAAGAAGTT TTCCTCCCAA AGAGGTGAGA AGGGACTATA GCAAAGGAGT AGCTGTAACT 
GAGAATGATG GAAAGAAGGA CAACAACAAG AGGAAAACAG AAACCAAGAA ATGCACCTTA 
GAAAAGTTAC AGGAAACAGG AAAGCAGAGT GTGGCAGTGC AGGTAAAATC TCAGACAGAG 
TTAAGAAAGA CTCCAGTGTC TGAAGCCAGG AAAACACCTG TAACTCAAAC CCCAAGTCAA 
GCAAGTAACT CCCAGTTCAT CCCCATTCAT CACCCTGGAG CCTTCCCTCC TCTTCCCAGC 
CGGCCAGGGT TCCCGCCCCC AACATATGTT ATCCCCCCTC CTGTGGCATT TTCTATGGGC 
TCAGGTTACA CCTTCCCAGC TGGTGTTTCT GTCCCAGGAA CCTTTCTTCA GCCTACAGCT 
CACTCTCCAC CAGGAAACCA GGTGCAAGCT GGGAAACAGT CCCACATTCC TTACAGCCAG 
CAACGGCCCT CCGGACCAGG GCCAGTGACC CAGGGACCTC AGCAGCCTCA GCCACCTTCC 
CAGCAGCCCC TCCCACCTCT GCCAGCTCCG CCAGCAGCAC AGACTGCAGG CCAGCTGCAG 
GTTCAAGCTC TAGCTCAGCA GCAACAGTCC CCTACAAAAG CTGTGCCGGC TTTGGGGAAA 
AGTCCTCCTC ACCACTCTGG ATTCCAGCAG TATCAACAGG CAGATGCCTC CAAACAGCTG 
TGGAATCCCC CTCAGGTTCA AGGCCCATTA GGGAAAATCA TGCCTGTGAA ACAGCCCTAC 
TACCTTCAAA CCCAAGACCC CATAAAACTG TTTGAGCCGT CGTTGCAACC TCCTGTAAGG 
CAGCAGCAGC CTCTAGAGAA AACAATGAAG CCTTTCCCCA TGGAGCCATA TAACCATAAC 
CCCTCAGAAG TCAAGATCCC GGAGTTCTAC TGGGATTCTT CCTACAGCGC TGCTGATAAC 
AGAGCTGTGA TGGCACAGCA AGCCAGTGTG GACCGCAGGG GCAAACGGTC CCCGGGAGTC 
TTCCGTCCAG AGCAGGACCC TGTGCCCAGG ATGCCATTTG AGAAATCCTT ACTGGAGAAG 
CCCTCAGAGC TCATGTCACA TTCATCCTCT TTCCTGTCCC TCACCGGATT CTCCCTCAAT 
CAGGAGCGAT ACCCAAATAA CAGTGTGTTC AATGAGGTAT ACGGGAAGAA CCTGACAACC 
AGCTCCAAAG CAGAACTTAA TCCCTCAGTG GCTCCCCAGG AAACCTCACT GTACTCGCTT 
TTTGAAGGGA CTCCATGGTC TCCATCTCTT CCTGCCAGTT CAGATCATTC AACACCAGCC 
AGCCAATCTC CTCATTCCTC CAACCCAAGC AGCCTGCCAA GCTCCCCTCC AACACACAAC 
CACAATTCTG TCCCATTCTC CAATTTTGGA CCCATTGGGA CTCCAGATAA CAGGGATAGG 
AGAACTGCAG ACCGGTGGAA AACTGATAAG CCAGCCATGG GTGGGTTTGG CATCGATTAT 
CTCTCAGCAA CATCATCCTC TGAGAGCAGT TGGCATCAGG CCAGCACTCC AAGTGGCTCC 
TGGACAGGCC ATGGCCCATC CATGGAGGAT TCCTCTGCTG TCCTCATGGA AAGCCTAAAG 
TCTATCTGGT CCAGTTCCAT GATGCATCCT GGACCTTCCG CTTTGGAGCA GCTGTTAATG 
CAGCAGAAGC AGAAACAGCA GCGGGGCCAA GGCGCCATGA ACCCTCCACA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG7 ( XM_005217296.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005217353.1 Bos taurus SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa27463
Clone ID Related Accession (Same CDS sequence)	XM_005217296.3
Accession Version	XM_005217296.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3651bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein SMG7 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104440.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005217296.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
CAGATGAGGA CCGGAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG 
GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCCGA AGTCTGGACA 
TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTTG TTACTGATTT GGAATACGCT 
TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCATGCCT TTAAGAATCA GATCACAACA 
CTACAAGGCC AGGCAAAGAA TCGAGCAAAC CCGAATCGGA GTGAAGTTCA GGCAAACCTT 
TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA 
GTGTTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AGTTGGGAAT TATCAGCAAT 
AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC 
CAGCACTGCC TCGTTCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA 
GAGTCCTACT ATAGGCATGC GGCTCAGCTT GTCCCCTCTA ATGGTCAGCC TTACAATCAG 
TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACTA CAATTTTCTA CTACTGCAGA 
AGCATTGCTG TGAAATTCCC TTTCCCAGCT GCCTCTACTA ATCTACAAAA AGCACTTTCT 
AAAGCACTGG AAAGCCGGGA TGAGGTGAAA ACCAAATGGG GTGTTTCTGA CTTCATCAAG 
GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT 
CTTCGAGAGA AGTTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAGAAAGC TTTCAACTCT 
CAGCAGTTAG TTCACGTCAC TGTCATTAAC CTGTTTCAAC TTCATCATCT TCGTGACTTT 
AGCAACGAAA CGGAGCAGCA TAGTTATAGC CAAGATGAGC AGCTGTGTTG GACACAGTTG 
CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTCCA GAACAAGTCT 
CAGGAGGAAT CCTACAATGC CTATCCCCTA CCTGCCGTCA AGGTGTCCAT GGACTGGCTG 
AGACTCAGAC CCAGGGTGTT TCAGGAAGCA GTGGTGGATG AAAGACAGTA CATTTGGCCG 
TGGCTAATTT CTCTTCTGAA CAGTTTCCAT CCCCATGAAG AAGATCTTTC AAGTACTAAT 
GCAACACCAC TTCCAGAGGA GTTTGAGTTA CAAGGATTCT TGGCTTTGAG ACCTTCTTTC 
AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GAGACAAAGA GGGTCAGCAA 
CGACAAATAC GACAGCAGCG TTTGATCTCT ATAGGCAAAT GGATTGCAGA TAATCAGCCA 
AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAG 
TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA CACAACCATC 
ATAGAGTCCC TGGCAGCTGA CGGGAACCCA GGACTGAAAT CAGTGCTATC TACAGGCCGG 
AATCTAAGCA ACAACTGCGA CCCAGGAGAG AAACCAGTGG TCACCTTCAA AGAGAACATG 
AAGCCACGAG AAGTGAGCAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTGAGAAGG 
GACTATAGCA AAGGAGTAGC TGTAACTGAG AATGATGGAA AGAAGGACAA CAACAAGAGG 
AAAACAGAAA CCAAGAAATG CACCTTAGAA AAGTTACAGG AAACAGGAAA GCAGAGTGTG 
GCAGTGCAGG TAAAATCTCA GACAGAGTTA AGAAAGACTC CAGTGTCTGA AGCCAGGAAA 
ACACCTGTAA CTCAAACCCC AAGTCAAGCA AGTAACTCCC AGTTCATCCC CATTCATCAC 
CCTGGAGCCT TCCCTCCTCT TCCCAGCCGG CCAGGGTTCC CGCCCCCAAC ATATGTTATC 
CCCCCTCCTG TGGCATTTTC TATGGGCTCA GGTTACACCT TCCCAGCTGG TGTTTCTGTC 
CCAGGAACCT TTCTTCAGCC TACAGCTCAC TCTCCACCAG GAAACCAGGT GCAAGCTGGG 
AAACAGTCCC ACATTCCTTA CAGCCAGCAA CGGCCCTCCG GACCAGGGCC AGTGACCCAG 
GGACCTCAGC AGCCTCAGCC ACCTTCCCAG CAGCCCCTCC CACCTCTGCC AGCTCCGCCA 
GCAGCACAGA CTGCAGGCCA GCTGCAGGTT CAAGCTCTAG CTCAGCAGCA ACAGTCCCCT 
ACAAAAGCTG TGCCGGCTTT GGGGAAAAGT CCTCCTCACC ACTCTGGATT CCAGCAGTAT 
CAACAGGCAG ATGCCTCCAA ACAGCTGTGG AATCCCCCTC AGGTTCAAGG CCCATTAGGG 
AAAATCATGC CTGTGAAACA GCCCTACTAC CTTCAAACCC AAGACCCCAT AAAACTGTTT 
GAGCCGTCGT TGCAACCTCC TGTAAGGCAG CAGCAGCCTC TAGAGAAAAC AATGAAGCCT 
TTCCCCATGG AGCCATATAA CCATAACCCC TCAGAAGTCA AGATCCCGGA GTTCTACTGG 
GATTCTTCCT ACAGCGCTGC TGATAACAGA GCTGTGATGG CACAGCAAGC CAGTGTGGAC 
CGCAGGGGCA AACGGTCCCC GGGAGTCTTC CGTCCAGAGC AGGACCCTGT GCCCAGGATG 
CCATTTGAGG ACCCCAAAGG CTCCCCTCTG CTTCCTCCGG ACCTGTTAAA GAGTCTGGCT 
GCCTTGGAGG AAGAGGAAGA GCTGATTTTT TCTAACCCTC CTGATCTTTA CCCAGCTCTG 
CTGGGGCCTC TCGCCTCTCT TCCTGGACGA AGCCTCTTTA AATCCTTACT GGAGAAGCCC 
TCAGAGCTCA TGTCACATTC ATCCTCTTTC CTGTCCCTCA CCGGATTCTC CCTCAATCAG 
GAGCGATACC CAAATAACAG TGTGTTCAAT GAGGTATACG GGAAGAACCT GACAACCAGC 
TCCAAAGCAG AACTTAATCC CTCAGTGGCT CCCCAGGAAA CCTCACTGTA CTCGCTTTTT 
GAAGGGACTC CATGGTCTCC ATCTCTTCCT GCCAGTTCAG ATCATTCAAC ACCAGCCAGC 
CAATCTCCTC ATTCCTCCAA CCCAAGCAGC CTGCCAAGCT CCCCTCCAAC ACACAACCAC 
AATTCTGTCC CATTCTCCAA TTTTGGACCC ATTGGGACTC CAGATAACAG GGATAGGAGA 
ACTGCAGACC GGTGGAAAAC TGATAAGCCA GCCATGGGTG GGTTTGGCAT CGATTATCTC 
TCAGCAACAT CATCCTCTGA GAGCAGTTGG CATCAGGCCA GCACTCCAAG TGGCTCCTGG 
ACAGGCCATG GCCCATCCAT GGAGGATTCC TCTGCTGTCC TCATGGAAAG CCTAAAGTCT 
ATCTGGTCCA GTTCCATGAT GCATCCTGGA CCTTCCGCTT TGGAGCAGCT GTTAATGCAG 
CAGAAGCAGA AACAGCAGCG GGGCCAAGGC GCCATGAACC CTCCACACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005217353.1
CDS	92..3742
Translation	MRNWMFLQIVIVGENVSFKSQMRTGNLKSEEHLKSSNIRQAEVLKADMTDSKLGPAEVWTSRQALQDLYQKMLVTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRANPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQTHTSAIVKPQSSSCSYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNLQKALSKALESRDEVKTKWGVSDFIKAFIKFHGHVYLSKSLEKLSPLREKLEEQFKRLLFQKAFNSQQLVHVTVINLFQLHHLRDFSNETEQHSYSQDEQLCWTQLLALFMSFLGILCKCPLQNKSQEESYNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPHEEDLSSTNATPLPEEFELQGFLALRPSFRNLDFSKGHQGITGDKEGQQRQIRQQRLISIGKWIADNQPRLIQCENEVGKLLFITEIPELILEDPSEAKENLILQDTTIIESLAADGNPGLKSVLSTGRNLSNNCDPGEKPVVTFKENMKPREVSRDQGRSFPPKEVRRDYSKGVAVTENDGKKDNNKRKTETKKCTLEKLQETGKQSVAVQVKSQTELRKTPVSEARKTPVTQTPSQASNSQFIPIHHPGAFPPLPSRPGFPPPTYVIPPPVAFSMGSGYTFPAGVSVPGTFLQPTAHSPPGNQVQAGKQSHIPYSQQRPSGPGPVTQGPQQPQPPSQQPLPPLPAPPAAQTAGQLQVQALAQQQQSPTKAVPALGKSPPHHSGFQQYQQADASKQLWNPPQVQGPLGKIMPVKQPYYLQTQDPIKLFEPSLQPPVRQQQPLEKTMKPFPMEPYNHNPSEVKIPEFYWDSSYSAADNRAVMAQQASVDRRGKRSPGVFRPEQDPVPRMPFEDPKGSPLLPPDLLKSLAALEEEEELIFSNPPDLYPALLGPLASLPGRSLFKSLLEKPSELMSHSSSFLSLTGFSLNQERYPNNSVFNEVYGKNLTTSSKAELNPSVAPQETSLYSLFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSVPFSNFGPIGTPDNRDRRTADRWKTDKPAMGGFGIDYLSATSSSESSWHQASTPSGSWTGHGPSMEDSSAVLMESLKSIWSSSMMHPGPSALEQLLMQQKQKQQRGQGAMNPPH

Target ORF information:

RefSeq Version	XM_005217296.3
Organism	Bos taurus(cattle)
Definition	Bos taurus SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005217296.3

ORF Insert Sequence:

ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
CAGATGAGGA CCGGAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG 
GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCCGA AGTCTGGACA 
TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTTG TTACTGATTT GGAATACGCT 
TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCATGCCT TTAAGAATCA GATCACAACA 
CTACAAGGCC AGGCAAAGAA TCGAGCAAAC CCGAATCGGA GTGAAGTTCA GGCAAACCTT 
TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA 
GTGTTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AGTTGGGAAT TATCAGCAAT 
AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC 
CAGCACTGCC TCGTTCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA 
GAGTCCTACT ATAGGCATGC GGCTCAGCTT GTCCCCTCTA ATGGTCAGCC TTACAATCAG 
TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACTA CAATTTTCTA CTACTGCAGA 
AGCATTGCTG TGAAATTCCC TTTCCCAGCT GCCTCTACTA ATCTACAAAA AGCACTTTCT 
AAAGCACTGG AAAGCCGGGA TGAGGTGAAA ACCAAATGGG GTGTTTCTGA CTTCATCAAG 
GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT 
CTTCGAGAGA AGTTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAGAAAGC TTTCAACTCT 
CAGCAGTTAG TTCACGTCAC TGTCATTAAC CTGTTTCAAC TTCATCATCT TCGTGACTTT 
AGCAACGAAA CGGAGCAGCA TAGTTATAGC CAAGATGAGC AGCTGTGTTG GACACAGTTG 
CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTCCA GAACAAGTCT 
CAGGAGGAAT CCTACAATGC CTATCCCCTA CCTGCCGTCA AGGTGTCCAT GGACTGGCTG 
AGACTCAGAC CCAGGGTGTT TCAGGAAGCA GTGGTGGATG AAAGACAGTA CATTTGGCCG 
TGGCTAATTT CTCTTCTGAA CAGTTTCCAT CCCCATGAAG AAGATCTTTC AAGTACTAAT 
GCAACACCAC TTCCAGAGGA GTTTGAGTTA CAAGGATTCT TGGCTTTGAG ACCTTCTTTC 
AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GAGACAAAGA GGGTCAGCAA 
CGACAAATAC GACAGCAGCG TTTGATCTCT ATAGGCAAAT GGATTGCAGA TAATCAGCCA 
AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAG 
TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA CACAACCATC 
ATAGAGTCCC TGGCAGCTGA CGGGAACCCA GGACTGAAAT CAGTGCTATC TACAGGCCGG 
AATCTAAGCA ACAACTGCGA CCCAGGAGAG AAACCAGTGG TCACCTTCAA AGAGAACATG 
AAGCCACGAG AAGTGAGCAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTGAGAAGG 
GACTATAGCA AAGGAGTAGC TGTAACTGAG AATGATGGAA AGAAGGACAA CAACAAGAGG 
AAAACAGAAA CCAAGAAATG CACCTTAGAA AAGTTACAGG AAACAGGAAA GCAGAGTGTG 
GCAGTGCAGG TAAAATCTCA GACAGAGTTA AGAAAGACTC CAGTGTCTGA AGCCAGGAAA 
ACACCTGTAA CTCAAACCCC AAGTCAAGCA AGTAACTCCC AGTTCATCCC CATTCATCAC 
CCTGGAGCCT TCCCTCCTCT TCCCAGCCGG CCAGGGTTCC CGCCCCCAAC ATATGTTATC 
CCCCCTCCTG TGGCATTTTC TATGGGCTCA GGTTACACCT TCCCAGCTGG TGTTTCTGTC 
CCAGGAACCT TTCTTCAGCC TACAGCTCAC TCTCCACCAG GAAACCAGGT GCAAGCTGGG 
AAACAGTCCC ACATTCCTTA CAGCCAGCAA CGGCCCTCCG GACCAGGGCC AGTGACCCAG 
GGACCTCAGC AGCCTCAGCC ACCTTCCCAG CAGCCCCTCC CACCTCTGCC AGCTCCGCCA 
GCAGCACAGA CTGCAGGCCA GCTGCAGGTT CAAGCTCTAG CTCAGCAGCA ACAGTCCCCT 
ACAAAAGCTG TGCCGGCTTT GGGGAAAAGT CCTCCTCACC ACTCTGGATT CCAGCAGTAT 
CAACAGGCAG ATGCCTCCAA ACAGCTGTGG AATCCCCCTC AGGTTCAAGG CCCATTAGGG 
AAAATCATGC CTGTGAAACA GCCCTACTAC CTTCAAACCC AAGACCCCAT AAAACTGTTT 
GAGCCGTCGT TGCAACCTCC TGTAAGGCAG CAGCAGCCTC TAGAGAAAAC AATGAAGCCT 
TTCCCCATGG AGCCATATAA CCATAACCCC TCAGAAGTCA AGATCCCGGA GTTCTACTGG 
GATTCTTCCT ACAGCGCTGC TGATAACAGA GCTGTGATGG CACAGCAAGC CAGTGTGGAC 
CGCAGGGGCA AACGGTCCCC GGGAGTCTTC CGTCCAGAGC AGGACCCTGT GCCCAGGATG 
CCATTTGAGG ACCCCAAAGG CTCCCCTCTG CTTCCTCCGG ACCTGTTAAA GAGTCTGGCT 
GCCTTGGAGG AAGAGGAAGA GCTGATTTTT TCTAACCCTC CTGATCTTTA CCCAGCTCTG 
CTGGGGCCTC TCGCCTCTCT TCCTGGACGA AGCCTCTTTA AATCCTTACT GGAGAAGCCC 
TCAGAGCTCA TGTCACATTC ATCCTCTTTC CTGTCCCTCA CCGGATTCTC CCTCAATCAG 
GAGCGATACC CAAATAACAG TGTGTTCAAT GAGGTATACG GGAAGAACCT GACAACCAGC 
TCCAAAGCAG AACTTAATCC CTCAGTGGCT CCCCAGGAAA CCTCACTGTA CTCGCTTTTT 
GAAGGGACTC CATGGTCTCC ATCTCTTCCT GCCAGTTCAG ATCATTCAAC ACCAGCCAGC 
CAATCTCCTC ATTCCTCCAA CCCAAGCAGC CTGCCAAGCT CCCCTCCAAC ACACAACCAC 
AATTCTGTCC CATTCTCCAA TTTTGGACCC ATTGGGACTC CAGATAACAG GGATAGGAGA 
ACTGCAGACC GGTGGAAAAC TGATAAGCCA GCCATGGGTG GGTTTGGCAT CGATTATCTC 
TCAGCAACAT CATCCTCTGA GAGCAGTTGG CATCAGGCCA GCACTCCAAG TGGCTCCTGG 
ACAGGCCATG GCCCATCCAT GGAGGATTCC TCTGCTGTCC TCATGGAAAG CCTAAAGTCT 
ATCTGGTCCA GTTCCATGAT GCATCCTGGA CCTTCCGCTT TGGAGCAGCT GTTAATGCAG 
CAGAAGCAGA AACAGCAGCG GGGCCAAGGC GCCATGAACC CTCCACACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG7 ( XM_005217297.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005217354.1 Bos taurus SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa27464
Clone ID Related Accession (Same CDS sequence)	XM_005217297.3
Accession Version	XM_005217297.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3564bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein SMG7 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104440.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005217297.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCTGC AGAGCGCGCA GTATCTCCGG CAGGCAGAAG TCCTGAAGGC TGACATGACA 
GATTCTAAGC TGGGTCCAGC CGAAGTCTGG ACATCCAGGC AGGCTCTGCA GGACCTGTAC 
CAGAAAATGC TTGTTACTGA TTTGGAATAC GCTTTAGACA AGAAAGTAGA ACAGGATCTC 
TGGAATCATG CCTTTAAGAA TCAGATCACA ACACTACAAG GCCAGGCAAA GAATCGAGCA 
AACCCGAATC GGAGTGAAGT TCAGGCAAAC CTTTCTCTGT TCCTAGAGGC AGCTAGTGGC 
TTCTATACTC AGTTATTACA AGAACTGTGT ACAGTGTTTA ATGTAGATTT ACCATGCCGT 
GTGAAGTCTT CCCAGTTGGG AATTATCAGC AATAAACAGA CGCATACCAG CGCCATAGTG 
AAGCCACAGT CTAGCTCCTG TTCCTATATC TGCCAGCACT GCCTCGTTCA CCTTGGAGAC 
ATTGCTCGAT ACAGAAACCA GACCAGCCAG GCAGAGTCCT ACTATAGGCA TGCGGCTCAG 
CTTGTCCCCT CTAATGGTCA GCCTTACAAT CAGTTGGCTA TCTTAGCTTC TTCCAAAGGA 
GACCATCTGA CTACAATTTT CTACTACTGC AGAAGCATTG CTGTGAAATT CCCTTTCCCA 
GCTGCCTCTA CTAATCTACA AAAAGCACTT TCTAAAGCAC TGGAAAGCCG GGATGAGGTG 
AAAACCAAAT GGGGTGTTTC TGACTTCATC AAGGCCTTTA TTAAATTCCA CGGTCATGTG 
TACCTGAGTA AGAGCTTGGA AAAGTTGAGC CCTCTTCGAG AGAAGTTGGA AGAACAGTTT 
AAGAGGCTGC TATTCCAGAA AGCTTTCAAC TCTCAGCAGT TAGTTCACGT CACTGTCATT 
AACCTGTTTC AACTTCATCA TCTTCGTGAC TTTAGCAACG AAACGGAGCA GCATAGTTAT 
AGCCAAGATG AGCAGCTGTG TTGGACACAG TTGCTGGCCC TCTTTATGTC TTTTCTTGGC 
ATCCTGTGCA AGTGTCCTCT CCAGAACAAG TCTCAGGAGG AATCCTACAA TGCCTATCCC 
CTACCTGCCG TCAAGGTGTC CATGGACTGG CTGAGACTCA GACCCAGGGT GTTTCAGGAA 
GCAGTGGTGG ATGAAAGACA GTACATTTGG CCGTGGCTAA TTTCTCTTCT GAACAGTTTC 
CATCCCCATG AAGAAGATCT TTCAAGTACT AATGCAACAC CACTTCCAGA GGAGTTTGAG 
TTACAAGGAT TCTTGGCTTT GAGACCTTCT TTCAGGAACT TGGATTTTTC CAAAGGTCAC 
CAGGGTATTA CAGGAGACAA AGAGGGTCAG CAACGACAAA TACGACAGCA GCGTTTGATC 
TCTATAGGCA AATGGATTGC AGATAATCAG CCAAGGCTGA TTCAGTGTGA AAATGAGGTA 
GGGAAATTGT TGTTTATCAC AGAAATCCCA GAGTTAATAC TGGAAGACCC CAGTGAAGCC 
AAAGAGAACC TCATTCTGCA AGACACAACC ATCATAGAGT CCCTGGCAGC TGACGGGAAC 
CCAGGACTGA AATCAGTGCT ATCTACAGGC CGGAATCTAA GCAACAACTG CGACCCAGGA 
GAGAAACCAG TGGTCACCTT CAAAGAGAAC ATGAAGCCAC GAGAAGTGAG CAGAGACCAA 
GGAAGAAGTT TTCCTCCCAA AGAGGTGAGA AGGGACTATA GCAAAGGAGT AGCTGTAACT 
GAGAATGATG GAAAGAAGGA CAACAACAAG AGGAAAACAG AAACCAAGAA ATGCACCTTA 
GAAAAGTTAC AGGAAACAGG AAAGCAGAGT GTGGCAGTGC AGGTAAAATC TCAGACAGAG 
TTAAGAAAGA CTCCAGTGTC TGAAGCCAGG AAAACACCTG TAACTCAAAC CCCAAGTCAA 
GCAAGTAACT CCCAGTTCAT CCCCATTCAT CACCCTGGAG CCTTCCCTCC TCTTCCCAGC 
CGGCCAGGGT TCCCGCCCCC AACATATGTT ATCCCCCCTC CTGTGGCATT TTCTATGGGC 
TCAGGTTACA CCTTCCCAGC TGGTGTTTCT GTCCCAGGAA CCTTTCTTCA GCCTACAGCT 
CACTCTCCAC CAGGAAACCA GGTGCAAGCT GGGAAACAGT CCCACATTCC TTACAGCCAG 
CAACGGCCCT CCGGACCAGG GCCAGTGACC CAGGGACCTC AGCAGCCTCA GCCACCTTCC 
CAGCAGCCCC TCCCACCTCT GCCAGCTCCG CCAGCAGCAC AGACTGCAGG CCAGCTGCAG 
GTTCAAGCTC TAGCTCAGCA GCAACAGTCC CCTACAAAAG CTGTGCCGGC TTTGGGGAAA 
AGTCCTCCTC ACCACTCTGG ATTCCAGCAG TATCAACAGG CAGATGCCTC CAAACAGCTG 
TGGAATCCCC CTCAGGTTCA AGGCCCATTA GGGAAAATCA TGCCTGTGAA ACAGCCCTAC 
TACCTTCAAA CCCAAGACCC CATAAAACTG TTTGAGCCGT CGTTGCAACC TCCTGTAAGG 
CAGCAGCAGC CTCTAGAGAA AACAATGAAG CCTTTCCCCA TGGAGCCATA TAACCATAAC 
CCCTCAGAAG TCAAGATCCC GGAGTTCTAC TGGGATTCTT CCTACAGCGC TGCTGATAAC 
AGAGCTGTGA TGGCACAGCA AGCCAGTGTG GACCGCAGGG GCAAACGGTC CCCGGGAGTC 
TTCCGTCCAG AGCAGGACCC TGTGCCCAGG ATGCCATTTG AGGACCCCAA AGGCTCCCCT 
CTGCTTCCTC CGGACCTGTT AAAGAGTCTG GCTGCCTTGG AGGAAGAGGA AGAGCTGATT 
TTTTCTAACC CTCCTGATCT TTACCCAGCT CTGCTGGGGC CTCTCGCCTC TCTTCCTGGA 
CGAAGCCTCT TTAAATCCTT ACTGGAGAAG CCCTCAGAGC TCATGTCACA TTCATCCTCT 
TTCCTGTCCC TCACCGGATT CTCCCTCAAT CAGGAGCGAT ACCCAAATAA CAGTGTGTTC 
AATGAGGTAT ACGGGAAGAA CCTGACAACC AGCTCCAAAG CAGAACTTAA TCCCTCAGTG 
GCTCCCCAGG AAACCTCACT GTACTCGCTT TTTGAAGGGA CTCCATGGTC TCCATCTCTT 
CCTGCCAGTT CAGATCATTC AACACCAGCC AGCCAATCTC CTCATTCCTC CAACCCAAGC 
AGCCTGCCAA GCTCCCCTCC AACACACAAC CACAATTCTG TCCCATTCTC CAATTTTGGA 
CCCATTGGGA CTCCAGATAA CAGGGATAGG AGAACTGCAG ACCGGTGGAA AACTGATAAG 
CCAGCCATGG GTGGGTTTGG CATCGATTAT CTCTCAGCAA CATCATCCTC TGAGAGCAGT 
TGGCATCAGG CCAGCACTCC AAGTGGCTCC TGGACAGGCC ATGGCCCATC CATGGAGGAT 
TCCTCTGCTG TCCTCATGGA AAGCCTAAAG TCTATCTGGT CCAGTTCCAT GATGCATCCT 
GGACCTTCCG CTTTGGAGCA GCTGTTAATG CAGCAGAAGC AGAAACAGCA GCGGGGCCAA 
GGCGCCATGA ACCCTCCACA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005217354.1
CDS	68..3631
Translation	MSLQSAQYLRQAEVLKADMTDSKLGPAEVWTSRQALQDLYQKMLVTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRANPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQTHTSAIVKPQSSSCSYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNLQKALSKALESRDEVKTKWGVSDFIKAFIKFHGHVYLSKSLEKLSPLREKLEEQFKRLLFQKAFNSQQLVHVTVINLFQLHHLRDFSNETEQHSYSQDEQLCWTQLLALFMSFLGILCKCPLQNKSQEESYNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPHEEDLSSTNATPLPEEFELQGFLALRPSFRNLDFSKGHQGITGDKEGQQRQIRQQRLISIGKWIADNQPRLIQCENEVGKLLFITEIPELILEDPSEAKENLILQDTTIIESLAADGNPGLKSVLSTGRNLSNNCDPGEKPVVTFKENMKPREVSRDQGRSFPPKEVRRDYSKGVAVTENDGKKDNNKRKTETKKCTLEKLQETGKQSVAVQVKSQTELRKTPVSEARKTPVTQTPSQASNSQFIPIHHPGAFPPLPSRPGFPPPTYVIPPPVAFSMGSGYTFPAGVSVPGTFLQPTAHSPPGNQVQAGKQSHIPYSQQRPSGPGPVTQGPQQPQPPSQQPLPPLPAPPAAQTAGQLQVQALAQQQQSPTKAVPALGKSPPHHSGFQQYQQADASKQLWNPPQVQGPLGKIMPVKQPYYLQTQDPIKLFEPSLQPPVRQQQPLEKTMKPFPMEPYNHNPSEVKIPEFYWDSSYSAADNRAVMAQQASVDRRGKRSPGVFRPEQDPVPRMPFEDPKGSPLLPPDLLKSLAALEEEEELIFSNPPDLYPALLGPLASLPGRSLFKSLLEKPSELMSHSSSFLSLTGFSLNQERYPNNSVFNEVYGKNLTTSSKAELNPSVAPQETSLYSLFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSVPFSNFGPIGTPDNRDRRTADRWKTDKPAMGGFGIDYLSATSSSESSWHQASTPSGSWTGHGPSMEDSSAVLMESLKSIWSSSMMHPGPSALEQLLMQQKQKQQRGQGAMNPPH

Target ORF information:

RefSeq Version	XM_005217297.3
Organism	Bos taurus(cattle)
Definition	Bos taurus SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005217297.3

ORF Insert Sequence:

ATGAGCCTGC AGAGCGCGCA GTATCTCCGG CAGGCAGAAG TCCTGAAGGC TGACATGACA 
GATTCTAAGC TGGGTCCAGC CGAAGTCTGG ACATCCAGGC AGGCTCTGCA GGACCTGTAC 
CAGAAAATGC TTGTTACTGA TTTGGAATAC GCTTTAGACA AGAAAGTAGA ACAGGATCTC 
TGGAATCATG CCTTTAAGAA TCAGATCACA ACACTACAAG GCCAGGCAAA GAATCGAGCA 
AACCCGAATC GGAGTGAAGT TCAGGCAAAC CTTTCTCTGT TCCTAGAGGC AGCTAGTGGC 
TTCTATACTC AGTTATTACA AGAACTGTGT ACAGTGTTTA ATGTAGATTT ACCATGCCGT 
GTGAAGTCTT CCCAGTTGGG AATTATCAGC AATAAACAGA CGCATACCAG CGCCATAGTG 
AAGCCACAGT CTAGCTCCTG TTCCTATATC TGCCAGCACT GCCTCGTTCA CCTTGGAGAC 
ATTGCTCGAT ACAGAAACCA GACCAGCCAG GCAGAGTCCT ACTATAGGCA TGCGGCTCAG 
CTTGTCCCCT CTAATGGTCA GCCTTACAAT CAGTTGGCTA TCTTAGCTTC TTCCAAAGGA 
GACCATCTGA CTACAATTTT CTACTACTGC AGAAGCATTG CTGTGAAATT CCCTTTCCCA 
GCTGCCTCTA CTAATCTACA AAAAGCACTT TCTAAAGCAC TGGAAAGCCG GGATGAGGTG 
AAAACCAAAT GGGGTGTTTC TGACTTCATC AAGGCCTTTA TTAAATTCCA CGGTCATGTG 
TACCTGAGTA AGAGCTTGGA AAAGTTGAGC CCTCTTCGAG AGAAGTTGGA AGAACAGTTT 
AAGAGGCTGC TATTCCAGAA AGCTTTCAAC TCTCAGCAGT TAGTTCACGT CACTGTCATT 
AACCTGTTTC AACTTCATCA TCTTCGTGAC TTTAGCAACG AAACGGAGCA GCATAGTTAT 
AGCCAAGATG AGCAGCTGTG TTGGACACAG TTGCTGGCCC TCTTTATGTC TTTTCTTGGC 
ATCCTGTGCA AGTGTCCTCT CCAGAACAAG TCTCAGGAGG AATCCTACAA TGCCTATCCC 
CTACCTGCCG TCAAGGTGTC CATGGACTGG CTGAGACTCA GACCCAGGGT GTTTCAGGAA 
GCAGTGGTGG ATGAAAGACA GTACATTTGG CCGTGGCTAA TTTCTCTTCT GAACAGTTTC 
CATCCCCATG AAGAAGATCT TTCAAGTACT AATGCAACAC CACTTCCAGA GGAGTTTGAG 
TTACAAGGAT TCTTGGCTTT GAGACCTTCT TTCAGGAACT TGGATTTTTC CAAAGGTCAC 
CAGGGTATTA CAGGAGACAA AGAGGGTCAG CAACGACAAA TACGACAGCA GCGTTTGATC 
TCTATAGGCA AATGGATTGC AGATAATCAG CCAAGGCTGA TTCAGTGTGA AAATGAGGTA 
GGGAAATTGT TGTTTATCAC AGAAATCCCA GAGTTAATAC TGGAAGACCC CAGTGAAGCC 
AAAGAGAACC TCATTCTGCA AGACACAACC ATCATAGAGT CCCTGGCAGC TGACGGGAAC 
CCAGGACTGA AATCAGTGCT ATCTACAGGC CGGAATCTAA GCAACAACTG CGACCCAGGA 
GAGAAACCAG TGGTCACCTT CAAAGAGAAC ATGAAGCCAC GAGAAGTGAG CAGAGACCAA 
GGAAGAAGTT TTCCTCCCAA AGAGGTGAGA AGGGACTATA GCAAAGGAGT AGCTGTAACT 
GAGAATGATG GAAAGAAGGA CAACAACAAG AGGAAAACAG AAACCAAGAA ATGCACCTTA 
GAAAAGTTAC AGGAAACAGG AAAGCAGAGT GTGGCAGTGC AGGTAAAATC TCAGACAGAG 
TTAAGAAAGA CTCCAGTGTC TGAAGCCAGG AAAACACCTG TAACTCAAAC CCCAAGTCAA 
GCAAGTAACT CCCAGTTCAT CCCCATTCAT CACCCTGGAG CCTTCCCTCC TCTTCCCAGC 
CGGCCAGGGT TCCCGCCCCC AACATATGTT ATCCCCCCTC CTGTGGCATT TTCTATGGGC 
TCAGGTTACA CCTTCCCAGC TGGTGTTTCT GTCCCAGGAA CCTTTCTTCA GCCTACAGCT 
CACTCTCCAC CAGGAAACCA GGTGCAAGCT GGGAAACAGT CCCACATTCC TTACAGCCAG 
CAACGGCCCT CCGGACCAGG GCCAGTGACC CAGGGACCTC AGCAGCCTCA GCCACCTTCC 
CAGCAGCCCC TCCCACCTCT GCCAGCTCCG CCAGCAGCAC AGACTGCAGG CCAGCTGCAG 
GTTCAAGCTC TAGCTCAGCA GCAACAGTCC CCTACAAAAG CTGTGCCGGC TTTGGGGAAA 
AGTCCTCCTC ACCACTCTGG ATTCCAGCAG TATCAACAGG CAGATGCCTC CAAACAGCTG 
TGGAATCCCC CTCAGGTTCA AGGCCCATTA GGGAAAATCA TGCCTGTGAA ACAGCCCTAC 
TACCTTCAAA CCCAAGACCC CATAAAACTG TTTGAGCCGT CGTTGCAACC TCCTGTAAGG 
CAGCAGCAGC CTCTAGAGAA AACAATGAAG CCTTTCCCCA TGGAGCCATA TAACCATAAC 
CCCTCAGAAG TCAAGATCCC GGAGTTCTAC TGGGATTCTT CCTACAGCGC TGCTGATAAC 
AGAGCTGTGA TGGCACAGCA AGCCAGTGTG GACCGCAGGG GCAAACGGTC CCCGGGAGTC 
TTCCGTCCAG AGCAGGACCC TGTGCCCAGG ATGCCATTTG AGGACCCCAA AGGCTCCCCT 
CTGCTTCCTC CGGACCTGTT AAAGAGTCTG GCTGCCTTGG AGGAAGAGGA AGAGCTGATT 
TTTTCTAACC CTCCTGATCT TTACCCAGCT CTGCTGGGGC CTCTCGCCTC TCTTCCTGGA 
CGAAGCCTCT TTAAATCCTT ACTGGAGAAG CCCTCAGAGC TCATGTCACA TTCATCCTCT 
TTCCTGTCCC TCACCGGATT CTCCCTCAAT CAGGAGCGAT ACCCAAATAA CAGTGTGTTC 
AATGAGGTAT ACGGGAAGAA CCTGACAACC AGCTCCAAAG CAGAACTTAA TCCCTCAGTG 
GCTCCCCAGG AAACCTCACT GTACTCGCTT TTTGAAGGGA CTCCATGGTC TCCATCTCTT 
CCTGCCAGTT CAGATCATTC AACACCAGCC AGCCAATCTC CTCATTCCTC CAACCCAAGC 
AGCCTGCCAA GCTCCCCTCC AACACACAAC CACAATTCTG TCCCATTCTC CAATTTTGGA 
CCCATTGGGA CTCCAGATAA CAGGGATAGG AGAACTGCAG ACCGGTGGAA AACTGATAAG 
CCAGCCATGG GTGGGTTTGG CATCGATTAT CTCTCAGCAA CATCATCCTC TGAGAGCAGT 
TGGCATCAGG CCAGCACTCC AAGTGGCTCC TGGACAGGCC ATGGCCCATC CATGGAGGAT 
TCCTCTGCTG TCCTCATGGA AAGCCTAAAG TCTATCTGGT CCAGTTCCAT GATGCATCCT 
GGACCTTCCG CTTTGGAGCA GCTGTTAATG CAGCAGAAGC AGAAACAGCA GCGGGGCCAA 
GGCGCCATGA ACCCTCCACA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	SMG7
Entrez Gene ID	537131
Full Name	SMG7, nonsense mediated mRNA decay factor
General protein information	Preferred Names SMG7, nonsense mediated mRNA decay factor Names protein SMG7 smg-7 homolog, nonsense mediated mRNA decay factor
Gene Type	protein-coding
Organism	Bos taurus(cattle)

	SMG7	XP_002802068.1
Mus musculus (house mouse)	Smg7	NP_001005507.1
Danio rerio (zebrafish)	smg7	NP_001038548.2
Bos taurus (cattle)	SMG7	NP_001180134.2
Xenopus tropicalis (tropical clawed frog)	smg7	NP_989258.1
Homo sapiens (human)	SMG7	NP_775179.1
Pan troglodytes (chimpanzee)	SMG7	XP_514048.3
Gallus gallus (chicken)	SMG7	XP_004943329.1
Canis lupus familiaris (dog)	SMG7	XP_537157.4
Rattus norvegicus (Norway rat)	Smg7	NP_001178478.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

SMG7 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

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