SMG7 cDNA ORF clone, Canis lupus familiaris(dog)

The following SMG7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SMG7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe09143 XM_022420965.1
Latest version!
Canis lupus familiaris SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe09145 XM_014115128.2
Latest version!
Canis lupus familiaris SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe09146 XM_014115129.2
Latest version!
Canis lupus familiaris SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe09147 XM_014115130.2
Latest version!
Canis lupus familiaris SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe37419 XM_014115127.2
Latest version!
Canis lupus familiaris SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe37420 XM_014115128.1
Latest version!
Canis lupus familiaris SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe37421 XM_014115129.1
Latest version!
Canis lupus familiaris SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe37422 XM_014115130.1
Latest version!
Canis lupus familiaris SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
View Old Accession Versions >>
OCe37419 XM_014115127.1 Canis lupus familiaris SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe09143
    Clone ID Related Accession (Same CDS sequence) XM_022420965.1
    Accession Version XM_022420965.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3678bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product protein SMG7 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGAGAGAAT GTCTTAAGCC ATTCTACCTT ATGAGAAATT GGATGTTCTT GCAGATAGTC 
    ATTGTGGGAG AAAACGTTTC ATATAAGTCC CAGATGAGGA CCGGAAACTT GAAATCAGAG
    GAGCATCTGA AATCAAGTAA TATTAGGCAG GCAGAAGTCC TGAAGGCTGA TATGACAGAT
    TCTAAGCTGG GTCCAGCTGA GGTCTGGACA TCCAGGCAGG CTCTGCAGGA CCTGTACCAG
    AAAATGCTAG TTACTGATTT GGAATACGCT TTAGACAAGA AAGTAGAACA GGATCTCTGG
    AATCATGCCT TTAAGAATCA GATCACAACA CTACAAGGCC AGGCAAAGAA TCGAGCAAAC
    CCGAATCGGA GTGAAGTTCA GGCAAACCTT TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC
    TATACTCAGT TATTACAAGA ACTGTGTACA GTGTTTAATG TAGATTTACC ATGCCGTGTG
    AAGTCTTCCC AATTGGGAAT TATCAGCAAT AAACAGACGC ATACCAGCGC CATAGTGAAG
    CCACAGTCTA GTTCCTGTTC TTATATCTGC CAGCACTGCC TCGTCCACCT TGGAGACATT
    GCTCGATACA GAAACCAGAC CAGCCAGGCA GAGTCCTACT ATAGGCATGC AGCTCAGCTT
    GTCCCCTCTA ATGGTCAGCC TTACAATCAG TTGGCTATCT TAGCTTCTTC CAAAGGAGAC
    CATCTGACCA CAATTTTCTA CTACTGCAGA AGCATCGCTG TGAAGTTCCC TTTCCCAGCT
    GCCTCCACGA ATCTACAAAA AGCACTTTCT AAAGCACTGG AAAGCCGGGA TGAGGTGAAA
    ACCAAATGGG GTGTTTCTGA CTTCATCAAG GCCTTTATTA AATTCCACGG TCATGTGTAC
    CTGAGTAAGA GCTTGGAAAA GCTGAGCCCT CTTCGAGAGA AATTGGAAGA ACAGTTTAAG
    AGGCTGCTAT TCCAAAAAGC TTTCAACTCT CAGCAGTTAG TTCATGTCAC TGTCATTAAC
    CTGTTTCAAC TTCATCACCT TCGTGACTTT AGCAATGAAA CGGAGCAGCA TAGTTATAGC
    CAAGATGAGC AGCTGTGTTG GACACAGTTA CTGGCCCTCT TTATGTCTTT TCTTGGCATC
    CTGTGCAAGT GTCCTCTCCA GAACAAGTCT CAGGAGGAGT CCTACAATGC CTATCCTCTT
    CCTGCAGTCA AGGTCTCCAT GGACTGGCTG AGACTCAGAC CCAGGGTCTT TCAGGAGGCA
    GTGGTAGATG AAAGACAGTA CATTTGGCCC TGGCTAATTT CTCTTCTAAA TAGTTTCCAT
    CCCCATGAAG AGGATCTTTC AAGTACTAAT GCTACACCAC TTCCAGAGGA GTTTGAATTA
    CAAGGATTCT TGGCTTTGAG ACCTTCTTTC AGGAACTTGG ATTTTTCCAA AGGCCATCAG
    GGTATTACAG GAGACAAAGA AGGTCAGCAA CGGCAAATAC GACAGCAGCG CTTGATCTCT
    ATAGGCAAAT GGATTGCCGA TAATCAGCCA AGGCTGATTC AGTGTGAAAA TGAGGTAGGG
    AAATTGTTGT TTATCACAGA AATCCCAGAG TTAACACTAG AAGACCCCAG TGAAGCCAAA
    GAGAACCTCA TTCTGCCAGA AACATCCATG ATAGAGTCAC TATCTGCAGA CGGGAACCCA
    GGACTGAAAT CAGTGCTGTC CACGGGCCGT AATCTAAGCA ACAACTGTGA CCCCGGAGAG
    AAACCTATGG TCACCTTCAA AGAGAACATT AAGCCGCGAG AAGTGAACAG AGACCAAGGA
    AGAAGTTTTC CTCCCAAAGA GGTGAGAAGG GACTATAGCA AAGGAATTAC TATAACTAAG
    AATGATGGAA AGAAGGACAA CAGCAAGAGG AAAACAGAAA CCAAGAAATG CACCTTAGAA
    AAGTTACAGG AAACAGGAAA GCAGAGTGTG GCAGTGCAGG TAAAATCCCA GACAGAATTA
    AGAAAGACTC CAGTGTCTGA AGCCAGGAAA ACCCCTGTAA CTCAAACCCC AAGTCAAGCA
    ACTAACTCCC AGTTCATCCC CATTCATCAC CCTGGAGCCT TCCCTCCTCT TCCCAGCCGG
    CCAGGGTTCC CGCCCCCTAC ATATGTTATT CCCCCTCCTG TGGCATTTTC TATGGGCTCA
    GGTTACACCT TCCCAGCTGG TGTTTCTGTC CCAGGAACCT TTCTTCAGCC TACAGCTCAC
    TCTCCAGCAG GAAACCAGGT GCAAGCTGGG AAACAGTCCC ACATTCCTTA CAGCCAGCAA
    CGGCCCTCTG GACCAGGGCC AATGAACCAG GGACCTCAAC AACCACAGCC ACCTTCCCAG
    CAATCCCTTA CATCTTTACC AGCTCAGCCA ACAGCACAGT CTACAAGCCA GTTGCAGGTT
    CAAGCTCTAG CTCAGCAACA ATCCCCCACA AAAGCTGTGC CAGCTTTGGG GAAAAGCCCT
    CCTCACCACT CTGGATTCCA GCAGTATCAA CAGGCAGATG CCTCCAAACA GCTGTGGAAT
    CCCCCTCAGG TTCAAGGCCC ATTAGGGAAA ATCATGCCTG TGAAACAGCC CTACTACCTT
    CAGTCCCAAG ACCCCATAAA ACTGTTTGAG CCGTCATTGC AACCTCCGGT AATGCAGCAG
    CAGCCTCTAG AGAAAAAAAT GAAGCCTTTT CCCATGGAGC CATATAACCA TAATCCCTCA
    GAAGTCAAGG TCCCAGAATT CTACTGGGAT TCTTCATACA GCATGGCTGA TAACAGAGCT
    GTAATGGCAC AGCAAGCAAA TATGGACCGC AGGGGCAAAC GGTCACCAGG AGTCTTCCGT
    CCAGAGCAGG ATCCTGTGCC CAGGATGCCA TTTGAGGACC CCAAGGGCTC CCCTCTGCTT
    CCTCCGGACC TGTTAAAGAG TCTGGCTGCC TTGGAGGAAG AGGAAGAGCT GATTTTTTCT
    AACCCTCCTG ATCTTTACCC AGCCCTGCTG GGGCCTCTCG CCTCTCTTCC TGGACGGAGC
    CTCTTTAAAT CCTTGTTGGA GAAGCCCTCG GAGCTCATGT CCCATTCCTC CTCTTTCTTG
    TCCCTCACCG GATTCTCTCT CAATCAGGAA AGATACCCAA ATAACAGTGT GTTTAATGAG
    GTATATGGGA AAAACCTGAC AACCAGCTCC AAATCAGAAC TTAATCCTTC GCTGGCCCCC
    CAGGAGACGT CACTCTATTC CCTCTTTGAA GGGACCCCCT GGTCTCCATC ACTTCCTGCC
    AGTTCAGATC ATTCAACACC AGCCAGCCAG TCTCCTCATT CCTCTAACCC AAGCAGCCTG
    CCAAGCTCCC CTCCAACACA CAACCATAAT TCTGTTCCAT TCTCCAACTT TGGACCCATT
    GGGACTCCAG ATAACAGGGA TAGGAGAACC GCAGATCGGT GGAAAACCGA TAAGCCAGCC
    ATGGGTGGGT TTGGCATTGA CTATCTCTCA GCAACATCAT CCTCTGAGAG CAGTTGGCAT
    CAGGCCAGCA CTGCAAGTGG CACCTGGACA GGCCACGGCC CCTCCATGGA GGATTCCTCT
    GCTGTCCTCA TGGAAAGCCT CAAGTCTATC TGGTCCAGTT CCATGATGCA TCCTGGACCT
    TCCGCTCTGG AGCAGCTGTT AATGCAGCAG AAGCAGAAGC AGCAGCGGGG ACAAGGCACC
    ATGAACCCTC CACACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022276673.1
    CDS118..3795
    Translation

    Target ORF information:

    RefSeq Version XM_022420965.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022420965.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGAGAGAAT GTCTTAAGCC ATTCTACCTT ATGAGAAATT GGATGTTCTT GCAGATAGTC 
    ATTGTGGGAG AAAACGTTTC ATATAAGTCC CAGATGAGGA CCGGAAACTT GAAATCAGAG
    GAGCATCTGA AATCAAGTAA TATTAGGCAG GCAGAAGTCC TGAAGGCTGA TATGACAGAT
    TCTAAGCTGG GTCCAGCTGA GGTCTGGACA TCCAGGCAGG CTCTGCAGGA CCTGTACCAG
    AAAATGCTAG TTACTGATTT GGAATACGCT TTAGACAAGA AAGTAGAACA GGATCTCTGG
    AATCATGCCT TTAAGAATCA GATCACAACA CTACAAGGCC AGGCAAAGAA TCGAGCAAAC
    CCGAATCGGA GTGAAGTTCA GGCAAACCTT TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC
    TATACTCAGT TATTACAAGA ACTGTGTACA GTGTTTAATG TAGATTTACC ATGCCGTGTG
    AAGTCTTCCC AATTGGGAAT TATCAGCAAT AAACAGACGC ATACCAGCGC CATAGTGAAG
    CCACAGTCTA GTTCCTGTTC TTATATCTGC CAGCACTGCC TCGTCCACCT TGGAGACATT
    GCTCGATACA GAAACCAGAC CAGCCAGGCA GAGTCCTACT ATAGGCATGC AGCTCAGCTT
    GTCCCCTCTA ATGGTCAGCC TTACAATCAG TTGGCTATCT TAGCTTCTTC CAAAGGAGAC
    CATCTGACCA CAATTTTCTA CTACTGCAGA AGCATCGCTG TGAAGTTCCC TTTCCCAGCT
    GCCTCCACGA ATCTACAAAA AGCACTTTCT AAAGCACTGG AAAGCCGGGA TGAGGTGAAA
    ACCAAATGGG GTGTTTCTGA CTTCATCAAG GCCTTTATTA AATTCCACGG TCATGTGTAC
    CTGAGTAAGA GCTTGGAAAA GCTGAGCCCT CTTCGAGAGA AATTGGAAGA ACAGTTTAAG
    AGGCTGCTAT TCCAAAAAGC TTTCAACTCT CAGCAGTTAG TTCATGTCAC TGTCATTAAC
    CTGTTTCAAC TTCATCACCT TCGTGACTTT AGCAATGAAA CGGAGCAGCA TAGTTATAGC
    CAAGATGAGC AGCTGTGTTG GACACAGTTA CTGGCCCTCT TTATGTCTTT TCTTGGCATC
    CTGTGCAAGT GTCCTCTCCA GAACAAGTCT CAGGAGGAGT CCTACAATGC CTATCCTCTT
    CCTGCAGTCA AGGTCTCCAT GGACTGGCTG AGACTCAGAC CCAGGGTCTT TCAGGAGGCA
    GTGGTAGATG AAAGACAGTA CATTTGGCCC TGGCTAATTT CTCTTCTAAA TAGTTTCCAT
    CCCCATGAAG AGGATCTTTC AAGTACTAAT GCTACACCAC TTCCAGAGGA GTTTGAATTA
    CAAGGATTCT TGGCTTTGAG ACCTTCTTTC AGGAACTTGG ATTTTTCCAA AGGCCATCAG
    GGTATTACAG GAGACAAAGA AGGTCAGCAA CGGCAAATAC GACAGCAGCG CTTGATCTCT
    ATAGGCAAAT GGATTGCCGA TAATCAGCCA AGGCTGATTC AGTGTGAAAA TGAGGTAGGG
    AAATTGTTGT TTATCACAGA AATCCCAGAG TTAACACTAG AAGACCCCAG TGAAGCCAAA
    GAGAACCTCA TTCTGCCAGA AACATCCATG ATAGAGTCAC TATCTGCAGA CGGGAACCCA
    GGACTGAAAT CAGTGCTGTC CACGGGCCGT AATCTAAGCA ACAACTGTGA CCCCGGAGAG
    AAACCTATGG TCACCTTCAA AGAGAACATT AAGCCGCGAG AAGTGAACAG AGACCAAGGA
    AGAAGTTTTC CTCCCAAAGA GGTGAGAAGG GACTATAGCA AAGGAATTAC TATAACTAAG
    AATGATGGAA AGAAGGACAA CAGCAAGAGG AAAACAGAAA CCAAGAAATG CACCTTAGAA
    AAGTTACAGG AAACAGGAAA GCAGAGTGTG GCAGTGCAGG TAAAATCCCA GACAGAATTA
    AGAAAGACTC CAGTGTCTGA AGCCAGGAAA ACCCCTGTAA CTCAAACCCC AAGTCAAGCA
    ACTAACTCCC AGTTCATCCC CATTCATCAC CCTGGAGCCT TCCCTCCTCT TCCCAGCCGG
    CCAGGGTTCC CGCCCCCTAC ATATGTTATT CCCCCTCCTG TGGCATTTTC TATGGGCTCA
    GGTTACACCT TCCCAGCTGG TGTTTCTGTC CCAGGAACCT TTCTTCAGCC TACAGCTCAC
    TCTCCAGCAG GAAACCAGGT GCAAGCTGGG AAACAGTCCC ACATTCCTTA CAGCCAGCAA
    CGGCCCTCTG GACCAGGGCC AATGAACCAG GGACCTCAAC AACCACAGCC ACCTTCCCAG
    CAATCCCTTA CATCTTTACC AGCTCAGCCA ACAGCACAGT CTACAAGCCA GTTGCAGGTT
    CAAGCTCTAG CTCAGCAACA ATCCCCCACA AAAGCTGTGC CAGCTTTGGG GAAAAGCCCT
    CCTCACCACT CTGGATTCCA GCAGTATCAA CAGGCAGATG CCTCCAAACA GCTGTGGAAT
    CCCCCTCAGG TTCAAGGCCC ATTAGGGAAA ATCATGCCTG TGAAACAGCC CTACTACCTT
    CAGTCCCAAG ACCCCATAAA ACTGTTTGAG CCGTCATTGC AACCTCCGGT AATGCAGCAG
    CAGCCTCTAG AGAAAAAAAT GAAGCCTTTT CCCATGGAGC CATATAACCA TAATCCCTCA
    GAAGTCAAGG TCCCAGAATT CTACTGGGAT TCTTCATACA GCATGGCTGA TAACAGAGCT
    GTAATGGCAC AGCAAGCAAA TATGGACCGC AGGGGCAAAC GGTCACCAGG AGTCTTCCGT
    CCAGAGCAGG ATCCTGTGCC CAGGATGCCA TTTGAGGACC CCAAGGGCTC CCCTCTGCTT
    CCTCCGGACC TGTTAAAGAG TCTGGCTGCC TTGGAGGAAG AGGAAGAGCT GATTTTTTCT
    AACCCTCCTG ATCTTTACCC AGCCCTGCTG GGGCCTCTCG CCTCTCTTCC TGGACGGAGC
    CTCTTTAAAT CCTTGTTGGA GAAGCCCTCG GAGCTCATGT CCCATTCCTC CTCTTTCTTG
    TCCCTCACCG GATTCTCTCT CAATCAGGAA AGATACCCAA ATAACAGTGT GTTTAATGAG
    GTATATGGGA AAAACCTGAC AACCAGCTCC AAATCAGAAC TTAATCCTTC GCTGGCCCCC
    CAGGAGACGT CACTCTATTC CCTCTTTGAA GGGACCCCCT GGTCTCCATC ACTTCCTGCC
    AGTTCAGATC ATTCAACACC AGCCAGCCAG TCTCCTCATT CCTCTAACCC AAGCAGCCTG
    CCAAGCTCCC CTCCAACACA CAACCATAAT TCTGTTCCAT TCTCCAACTT TGGACCCATT
    GGGACTCCAG ATAACAGGGA TAGGAGAACC GCAGATCGGT GGAAAACCGA TAAGCCAGCC
    ATGGGTGGGT TTGGCATTGA CTATCTCTCA GCAACATCAT CCTCTGAGAG CAGTTGGCAT
    CAGGCCAGCA CTGCAAGTGG CACCTGGACA GGCCACGGCC CCTCCATGGA GGATTCCTCT
    GCTGTCCTCA TGGAAAGCCT CAAGTCTATC TGGTCCAGTT CCATGATGCA TCCTGGACCT
    TCCGCTCTGG AGCAGCTGTT AATGCAGCAG AAGCAGAAGC AGCAGCGGGG ACAAGGCACC
    ATGAACCCTC CACACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe09145
    Clone ID Related Accession (Same CDS sequence) XM_014115128.2
    Accession Version XM_014115128.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3540bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product protein SMG7 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014115128.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGAGAAT GTCTTAAGCC ATTCTACCTT ATGAGAAATT GGATGTTCTT GCAGATAGTC 
    ATTGTGGGAG AAAACGTTTC ATATAAGTCC CAGATGAGGA CCGGAAACTT GAAATCAGAG
    GAGCATCTGA AATCAAGTAA TATTAGGCAG GCAGAAGTCC TGAAGGCTGA TATGACAGAT
    TCTAAGCTGG GTCCAGCTGA GGTCTGGACA TCCAGGCAGG CTCTGCAGGA CCTGTACCAG
    AAAATGCTAG TTACTGATTT GGAATACGCT TTAGACAAGA AAGTAGAACA GGATCTCTGG
    AATCATGCCT TTAAGAATCA GATCACAACA CTACAAGGCC AGGCAAAGAA TCGAGCAAAC
    CCGAATCGGA GTGAAGTTCA GGCAAACCTT TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC
    TATACTCAGT TATTACAAGA ACTGTGTACA GTGTTTAATG TAGATTTACC ATGCCGTGTG
    AAGTCTTCCC AATTGGGAAT TATCAGCAAT AAACAGACGC ATACCAGCGC CATAGTGAAG
    CCACAGTCTA GTTCCTGTTC TTATATCTGC CAGCACTGCC TCGTCCACCT TGGAGACATT
    GCTCGATACA GAAACCAGAC CAGCCAGGCA GAGTCCTACT ATAGGCATGC AGCTCAGCTT
    GTCCCCTCTA ATGGTCAGCC TTACAATCAG TTGGCTATCT TAGCTTCTTC CAAAGGAGAC
    CATCTGACCA CAATTTTCTA CTACTGCAGA AGCATCGCTG TGAAGTTCCC TTTCCCAGCT
    GCCTCCACGA ATCTACAAAA AGCACTTTCT AAAGCACTGG AAAGCCGGGA TGAGGTGAAA
    ACCAAATGGG GTGTTTCTGA CTTCATCAAG GCCTTTATTA AATTCCACGG TCATGTGTAC
    CTGAGTAAGA GCTTGGAAAA GCTGAGCCCT CTTCGAGAGA AATTGGAAGA ACAGTTTAAG
    AGGCTGCTAT TCCAAAAAGC TTTCAACTCT CAGCAGTTAG TTCATGTCAC TGTCATTAAC
    CTGTTTCAAC TTCATCACCT TCGTGACTTT AGCAATGAAA CGGAGCAGCA TAGTTATAGC
    CAAGATGAGC AGCTGTGTTG GACACAGTTA CTGGCCCTCT TTATGTCTTT TCTTGGCATC
    CTGTGCAAGT GTCCTCTCCA GAACAAGTCT CAGGAGGAGT CCTACAATGC CTATCCTCTT
    CCTGCAGTCA AGGTCTCCAT GGACTGGCTG AGACTCAGAC CCAGGGTCTT TCAGGAGGCA
    GTGGTAGATG AAAGACAGTA CATTTGGCCC TGGCTAATTT CTCTTCTAAA TAGTTTCCAT
    CCCCATGAAG AGGATCTTTC AAGTACTAAT GCTACACCAC TTCCAGAGGA GTTTGAATTA
    CAAGGATTCT TGGCTTTGAG ACCTTCTTTC AGGAACTTGG ATTTTTCCAA AGGCCATCAG
    GGTATTACAG GAGACAAAGA AGGTCAGCAA CGGCAAATAC GACAGCAGCG CTTGATCTCT
    ATAGGCAAAT GGATTGCCGA TAATCAGCCA AGGCTGATTC AGTGTGAAAA TGAGGTAGGG
    AAATTGTTGT TTATCACAGA AATCCCAGAG TTAACACTAG AAGACCCCAG TGAAGCCAAA
    GAGAACCTCA TTCTGCCAGA AACATCCATG ATAGAGTCAC TATCTGCAGA CGGGAACCCA
    GGACTGAAAT CAGTGCTGTC CACGGGCCGT AATCTAAGCA ACAACTGTGA CCCCGGAGAG
    AAACCTATGG TCACCTTCAA AGAGAACATT AAGCCGCGAG AAGTGAACAG AGACCAAGGA
    AGAAGTTTTC CTCCCAAAGA GGTAAAATCC CAGACAGAAT TAAGAAAGAC TCCAGTGTCT
    GAAGCCAGGA AAACCCCTGT AACTCAAACC CCAAGTCAAG CAACTAACTC CCAGTTCATC
    CCCATTCATC ACCCTGGAGC CTTCCCTCCT CTTCCCAGCC GGCCAGGGTT CCCGCCCCCT
    ACATATGTTA TTCCCCCTCC TGTGGCATTT TCTATGGGCT CAGGTTACAC CTTCCCAGCT
    GGTGTTTCTG TCCCAGGAAC CTTTCTTCAG CCTACAGCTC ACTCTCCAGC AGGAAACCAG
    GTGCAAGCTG GGAAACAGTC CCACATTCCT TACAGCCAGC AACGGCCCTC TGGACCAGGG
    CCAATGAACC AGGGACCTCA ACAACCACAG CCACCTTCCC AGCAATCCCT TACATCTTTA
    CCAGCTCAGC CAACAGCACA GTCTACAAGC CAGTTGCAGG TTCAAGCTCT AGCTCAGCAA
    CAATCCCCCA CAAAAGCTGT GCCAGCTTTG GGGAAAAGCC CTCCTCACCA CTCTGGATTC
    CAGCAGTATC AACAGGCAGA TGCCTCCAAA CAGCTGTGGA ATCCCCCTCA GGTTCAAGGC
    CCATTAGGGA AAATCATGCC TGTGAAACAG CCCTACTACC TTCAGTCCCA AGACCCCATA
    AAACTGTTTG AGCCGTCATT GCAACCTCCG GTAATGCAGC AGCAGCCTCT AGAGAAAAAA
    ATGAAGCCTT TTCCCATGGA GCCATATAAC CATAATCCCT CAGAAGTCAA GGTCCCAGAA
    TTCTACTGGG ATTCTTCATA CAGCATGGCT GATAACAGAG CTGTAATGGC ACAGCAAGCA
    AATATGGACC GCAGGGGCAA ACGGTCACCA GGAGTCTTCC GTCCAGAGCA GGATCCTGTG
    CCCAGGATGC CATTTGAGGA CCCCAAGGGC TCCCCTCTGC TTCCTCCGGA CCTGTTAAAG
    AGTCTGGCTG CCTTGGAGGA AGAGGAAGAG CTGATTTTTT CTAACCCTCC TGATCTTTAC
    CCAGCCCTGC TGGGGCCTCT CGCCTCTCTT CCTGGACGGA GCCTCTTTAA ATCCTTGTTG
    GAGAAGCCCT CGGAGCTCAT GTCCCATTCC TCCTCTTTCT TGTCCCTCAC CGGATTCTCT
    CTCAATCAGG AAAGATACCC AAATAACAGT GTGTTTAATG AGGTATATGG GAAAAACCTG
    ACAACCAGCT CCAAATCAGA ACTTAATCCT TCGCTGGCCC CCCAGGAGAC GTCACTCTAT
    TCCCTCTTTG AAGGGACCCC CTGGTCTCCA TCACTTCCTG CCAGTTCAGA TCATTCAACA
    CCAGCCAGCC AGTCTCCTCA TTCCTCTAAC CCAAGCAGCC TGCCAAGCTC CCCTCCAACA
    CACAACCATA ATTCTGTTCC ATTCTCCAAC TTTGGACCCA TTGGGACTCC AGATAACAGG
    GATAGGAGAA CCGCAGATCG GTGGAAAACC GATAAGCCAG CCATGGGTGG GTTTGGCATT
    GACTATCTCT CAGCAACATC ATCCTCTGAG AGCAGTTGGC ATCAGGCCAG CACTGCAAGT
    GGCACCTGGA CAGGCCACGG CCCCTCCATG GAGGATTCCT CTGCTGTCCT CATGGAAAGC
    CTCAAGTCTA TCTGGTCCAG TTCCATGATG CATCCTGGAC CTTCCGCTCT GGAGCAGCTG
    TTAATGCAGC AGAAGCAGAA GCAGCAGCGG GGACAAGGCA CCATGAACCC TCCACACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013970603.2
    CDS115..3654
    Translation

    Target ORF information:

    RefSeq Version XM_014115128.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014115128.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGAGAAT GTCTTAAGCC ATTCTACCTT ATGAGAAATT GGATGTTCTT GCAGATAGTC 
    ATTGTGGGAG AAAACGTTTC ATATAAGTCC CAGATGAGGA CCGGAAACTT GAAATCAGAG
    GAGCATCTGA AATCAAGTAA TATTAGGCAG GCAGAAGTCC TGAAGGCTGA TATGACAGAT
    TCTAAGCTGG GTCCAGCTGA GGTCTGGACA TCCAGGCAGG CTCTGCAGGA CCTGTACCAG
    AAAATGCTAG TTACTGATTT GGAATACGCT TTAGACAAGA AAGTAGAACA GGATCTCTGG
    AATCATGCCT TTAAGAATCA GATCACAACA CTACAAGGCC AGGCAAAGAA TCGAGCAAAC
    CCGAATCGGA GTGAAGTTCA GGCAAACCTT TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC
    TATACTCAGT TATTACAAGA ACTGTGTACA GTGTTTAATG TAGATTTACC ATGCCGTGTG
    AAGTCTTCCC AATTGGGAAT TATCAGCAAT AAACAGACGC ATACCAGCGC CATAGTGAAG
    CCACAGTCTA GTTCCTGTTC TTATATCTGC CAGCACTGCC TCGTCCACCT TGGAGACATT
    GCTCGATACA GAAACCAGAC CAGCCAGGCA GAGTCCTACT ATAGGCATGC AGCTCAGCTT
    GTCCCCTCTA ATGGTCAGCC TTACAATCAG TTGGCTATCT TAGCTTCTTC CAAAGGAGAC
    CATCTGACCA CAATTTTCTA CTACTGCAGA AGCATCGCTG TGAAGTTCCC TTTCCCAGCT
    GCCTCCACGA ATCTACAAAA AGCACTTTCT AAAGCACTGG AAAGCCGGGA TGAGGTGAAA
    ACCAAATGGG GTGTTTCTGA CTTCATCAAG GCCTTTATTA AATTCCACGG TCATGTGTAC
    CTGAGTAAGA GCTTGGAAAA GCTGAGCCCT CTTCGAGAGA AATTGGAAGA ACAGTTTAAG
    AGGCTGCTAT TCCAAAAAGC TTTCAACTCT CAGCAGTTAG TTCATGTCAC TGTCATTAAC
    CTGTTTCAAC TTCATCACCT TCGTGACTTT AGCAATGAAA CGGAGCAGCA TAGTTATAGC
    CAAGATGAGC AGCTGTGTTG GACACAGTTA CTGGCCCTCT TTATGTCTTT TCTTGGCATC
    CTGTGCAAGT GTCCTCTCCA GAACAAGTCT CAGGAGGAGT CCTACAATGC CTATCCTCTT
    CCTGCAGTCA AGGTCTCCAT GGACTGGCTG AGACTCAGAC CCAGGGTCTT TCAGGAGGCA
    GTGGTAGATG AAAGACAGTA CATTTGGCCC TGGCTAATTT CTCTTCTAAA TAGTTTCCAT
    CCCCATGAAG AGGATCTTTC AAGTACTAAT GCTACACCAC TTCCAGAGGA GTTTGAATTA
    CAAGGATTCT TGGCTTTGAG ACCTTCTTTC AGGAACTTGG ATTTTTCCAA AGGCCATCAG
    GGTATTACAG GAGACAAAGA AGGTCAGCAA CGGCAAATAC GACAGCAGCG CTTGATCTCT
    ATAGGCAAAT GGATTGCCGA TAATCAGCCA AGGCTGATTC AGTGTGAAAA TGAGGTAGGG
    AAATTGTTGT TTATCACAGA AATCCCAGAG TTAACACTAG AAGACCCCAG TGAAGCCAAA
    GAGAACCTCA TTCTGCCAGA AACATCCATG ATAGAGTCAC TATCTGCAGA CGGGAACCCA
    GGACTGAAAT CAGTGCTGTC CACGGGCCGT AATCTAAGCA ACAACTGTGA CCCCGGAGAG
    AAACCTATGG TCACCTTCAA AGAGAACATT AAGCCGCGAG AAGTGAACAG AGACCAAGGA
    AGAAGTTTTC CTCCCAAAGA GGTAAAATCC CAGACAGAAT TAAGAAAGAC TCCAGTGTCT
    GAAGCCAGGA AAACCCCTGT AACTCAAACC CCAAGTCAAG CAACTAACTC CCAGTTCATC
    CCCATTCATC ACCCTGGAGC CTTCCCTCCT CTTCCCAGCC GGCCAGGGTT CCCGCCCCCT
    ACATATGTTA TTCCCCCTCC TGTGGCATTT TCTATGGGCT CAGGTTACAC CTTCCCAGCT
    GGTGTTTCTG TCCCAGGAAC CTTTCTTCAG CCTACAGCTC ACTCTCCAGC AGGAAACCAG
    GTGCAAGCTG GGAAACAGTC CCACATTCCT TACAGCCAGC AACGGCCCTC TGGACCAGGG
    CCAATGAACC AGGGACCTCA ACAACCACAG CCACCTTCCC AGCAATCCCT TACATCTTTA
    CCAGCTCAGC CAACAGCACA GTCTACAAGC CAGTTGCAGG TTCAAGCTCT AGCTCAGCAA
    CAATCCCCCA CAAAAGCTGT GCCAGCTTTG GGGAAAAGCC CTCCTCACCA CTCTGGATTC
    CAGCAGTATC AACAGGCAGA TGCCTCCAAA CAGCTGTGGA ATCCCCCTCA GGTTCAAGGC
    CCATTAGGGA AAATCATGCC TGTGAAACAG CCCTACTACC TTCAGTCCCA AGACCCCATA
    AAACTGTTTG AGCCGTCATT GCAACCTCCG GTAATGCAGC AGCAGCCTCT AGAGAAAAAA
    ATGAAGCCTT TTCCCATGGA GCCATATAAC CATAATCCCT CAGAAGTCAA GGTCCCAGAA
    TTCTACTGGG ATTCTTCATA CAGCATGGCT GATAACAGAG CTGTAATGGC ACAGCAAGCA
    AATATGGACC GCAGGGGCAA ACGGTCACCA GGAGTCTTCC GTCCAGAGCA GGATCCTGTG
    CCCAGGATGC CATTTGAGGA CCCCAAGGGC TCCCCTCTGC TTCCTCCGGA CCTGTTAAAG
    AGTCTGGCTG CCTTGGAGGA AGAGGAAGAG CTGATTTTTT CTAACCCTCC TGATCTTTAC
    CCAGCCCTGC TGGGGCCTCT CGCCTCTCTT CCTGGACGGA GCCTCTTTAA ATCCTTGTTG
    GAGAAGCCCT CGGAGCTCAT GTCCCATTCC TCCTCTTTCT TGTCCCTCAC CGGATTCTCT
    CTCAATCAGG AAAGATACCC AAATAACAGT GTGTTTAATG AGGTATATGG GAAAAACCTG
    ACAACCAGCT CCAAATCAGA ACTTAATCCT TCGCTGGCCC CCCAGGAGAC GTCACTCTAT
    TCCCTCTTTG AAGGGACCCC CTGGTCTCCA TCACTTCCTG CCAGTTCAGA TCATTCAACA
    CCAGCCAGCC AGTCTCCTCA TTCCTCTAAC CCAAGCAGCC TGCCAAGCTC CCCTCCAACA
    CACAACCATA ATTCTGTTCC ATTCTCCAAC TTTGGACCCA TTGGGACTCC AGATAACAGG
    GATAGGAGAA CCGCAGATCG GTGGAAAACC GATAAGCCAG CCATGGGTGG GTTTGGCATT
    GACTATCTCT CAGCAACATC ATCCTCTGAG AGCAGTTGGC ATCAGGCCAG CACTGCAAGT
    GGCACCTGGA CAGGCCACGG CCCCTCCATG GAGGATTCCT CTGCTGTCCT CATGGAAAGC
    CTCAAGTCTA TCTGGTCCAG TTCCATGATG CATCCTGGAC CTTCCGCTCT GGAGCAGCTG
    TTAATGCAGC AGAAGCAGAA GCAGCAGCGG GGACAAGGCA CCATGAACCC TCCACACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe09146
    Clone ID Related Accession (Same CDS sequence) XM_014115129.2
    Accession Version XM_014115129.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3528bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product protein SMG7 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014115129.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGAGAAT GTCTTAAGCC ATTCTACCTT ATGAGAAATT GGATGTTCTT GCAGATAGTC 
    ATTGTGGGAG AAAACGTTTC ATATAAGTCC CAGATGAGGA CCGGAAACTT GAAATCAGAG
    GAGCATCTGA AATCAAGTAA TATTAGGCAG GCAGAAGTCC TGAAGGCTGA TATGACAGAT
    TCTAAGCTGG GTCCAGCTGA GGTCTGGACA TCCAGGCAGG CTCTGCAGGA CCTGTACCAG
    AAAATGCTAG TTACTGATTT GGAATACGCT TTAGACAAGA AAGTAGAACA GGATCTCTGG
    AATCATGCCT TTAAGAATCA GATCACAACA CTACAAGGCC AGGCAAAGAA TCGAGCAAAC
    CCGAATCGGA GTGAAGTTCA GGCAAACCTT TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC
    TATACTCAGT TATTACAAGA ACTGTGTACA GTGTTTAATG TAGATTTACC ATGCCGTGTG
    AAGTCTTCCC AATTGGGAAT TATCAGCAAT AAACAGACGC ATACCAGCGC CATAGTGAAG
    CCACAGTCTA GTTCCTGTTC TTATATCTGC CAGCACTGCC TCGTCCACCT TGGAGACATT
    GCTCGATACA GAAACCAGAC CAGCCAGGCA GAGTCCTACT ATAGGCATGC AGCTCAGCTT
    GTCCCCTCTA ATGGTCAGCC TTACAATCAG TTGGCTATCT TAGCTTCTTC CAAAGGAGAC
    CATCTGACCA CAATTTTCTA CTACTGCAGA AGCATCGCTG TGAAGTTCCC TTTCCCAGCT
    GCCTCCACGA ATCTACAAAA AGCACTTTCT AAAGCACTGG AAAGCCGGGA TGAGGTGAAA
    ACCAAATGGG GTGTTTCTGA CTTCATCAAG GCCTTTATTA AATTCCACGG TCATGTGTAC
    CTGAGTAAGA GCTTGGAAAA GCTGAGCCCT CTTCGAGAGA AATTGGAAGA ACAGTTTAAG
    AGGCTGCTAT TCCAAAAAGC TTTCAACTCT CAGCAGTTAG TTCATGTCAC TGTCATTAAC
    CTGTTTCAAC TTCATCACCT TCGTGACTTT AGCAATGAAA CGGAGCAGCA TAGTTATAGC
    CAAGATGAGC AGCTGTGTTG GACACAGTTA CTGGCCCTCT TTATGTCTTT TCTTGGCATC
    CTGTGCAAGT GTCCTCTCCA GAACAAGTCT CAGGAGGAGT CCTACAATGC CTATCCTCTT
    CCTGCAGTCA AGGTCTCCAT GGACTGGCTG AGACTCAGAC CCAGGGTCTT TCAGGAGGCA
    GTGGTAGATG AAAGACAGTA CATTTGGCCC TGGCTAATTT CTCTTCTAAA TAGTTTCCAT
    CCCCATGAAG AGGATCTTTC AAGTACTAAT GCTACACCAC TTCCAGAGGA GTTTGAATTA
    CAAGGATTCT TGGCTTTGAG ACCTTCTTTC AGGAACTTGG ATTTTTCCAA AGGCCATCAG
    GGTATTACAG GAGACAAAGA AGGTCAGCAA CGGCAAATAC GACAGCAGCG CTTGATCTCT
    ATAGGCAAAT GGATTGCCGA TAATCAGCCA AGGCTGATTC AGTGTGAAAA TGAGGTAGGG
    AAATTGTTGT TTATCACAGA AATCCCAGAG TTAACACTAG AAGACCCCAG TGAAGCCAAA
    GAGAACCTCA TTCTGCCAGA AACATCCATG ATAGAGTCAC TATCTGCAGA CGGGAACCCA
    GGACTGAAAT CAGTGCTGTC CACGGGCCGT AATCTAAGCA ACAACTGTGA CCCCGGAGAG
    AAACCTATGG TCACCTTCAA AGAGAACATT AAGCCGCGAG AAGTGAACAG AGACCAAGGA
    AGAAGTTTTC CTCCCAAAGA GGTGAGAAGG GACTATAGCA AAGGAATTAC TATAACTAAG
    AATGATGGAA AGAAGGACAA CAGCAAGAGG AAAACAGAAA CCAAGAAATG CACCTTAGAA
    AAGTTACAGG AAACAGGAAA GCAGAGTGTG GCAGTGCAGG TAAAATCCCA GACAGAATTA
    AGAAAGACTC CAGTGTCTGA AGCCAGGAAA ACCCCTGTAA CTCAAACCCC AAGTCAAGCA
    ACTAACTCCC AGTTCATCCC CATTCATCAC CCTGGAGCCT TCCCTCCTCT TCCCAGCCGG
    CCAGGGTTCC CGCCCCCTAC ATATGTTATT CCCCCTCCTG TGGCATTTTC TATGGGCTCA
    GGTTACACCT TCCCAGCTGG TGTTTCTGTC CCAGGAACCT TTCTTCAGCC TACAGCTCAC
    TCTCCAGCAG GAAACCAGGT GCAAGCTGGG AAACAGTCCC ACATTCCTTA CAGCCAGCAA
    CGGCCCTCTG GACCAGGGCC AATGAACCAG GGACCTCAAC AACCACAGCC ACCTTCCCAG
    CAATCCCTTA CATCTTTACC AGCTCAGCCA ACAGCACAGT CTACAAGCCA GTTGCAGGTT
    CAAGCTCTAG CTCAGCAACA ATCCCCCACA AAAGCTGTGC CAGCTTTGGG GAAAAGCCCT
    CCTCACCACT CTGGATTCCA GCAGTATCAA CAGGCAGATG CCTCCAAACA GCTGTGGAAT
    CCCCCTCAGG TTCAAGGCCC ATTAGGGAAA ATCATGCCTG TGAAACAGCC CTACTACCTT
    CAGTCCCAAG ACCCCATAAA ACTGTTTGAG CCGTCATTGC AACCTCCGGT AATGCAGCAG
    CAGCCTCTAG AGAAAAAAAT GAAGCCTTTT CCCATGGAGC CATATAACCA TAATCCCTCA
    GAAGTCAAGG TCCCAGAATT CTACTGGGAT TCTTCATACA GCATGGCTGA TAACAGAGCT
    GTAATGGCAC AGCAAGCAAA TATGGACCGC AGGGGCAAAC GGTCACCAGG AGTCTTCCGT
    CCAGAGCAGG ATCCTGTGCC CAGGATGCCA TTTGAGAAAT CCTTGTTGGA GAAGCCCTCG
    GAGCTCATGT CCCATTCCTC CTCTTTCTTG TCCCTCACCG GATTCTCTCT CAATCAGGAA
    AGATACCCAA ATAACAGTGT GTTTAATGAG GTATATGGGA AAAACCTGAC AACCAGCTCC
    AAATCAGAAC TTAATCCTTC GCTGGCCCCC CAGGAGACGT CACTCTATTC CCTCTTTGAA
    GGGACCCCCT GGTCTCCATC ACTTCCTGCC AGTTCAGATC ATTCAACACC AGCCAGCCAG
    TCTCCTCATT CCTCTAACCC AAGCAGCCTG CCAAGCTCCC CTCCAACACA CAACCATAAT
    TCTGTTCCAT TCTCCAACTT TGGACCCATT GGGACTCCAG ATAACAGGGA TAGGAGAACC
    GCAGATCGGT GGAAAACCGA TAAGCCAGCC ATGGGTGGGT TTGGCATTGA CTATCTCTCA
    GCAACATCAT CCTCTGAGAG CAGTTGGCAT CAGGCCAGCA CTGCAAGTGG CACCTGGACA
    GGCCACGGCC CCTCCATGGA GGATTCCTCT GCTGTCCTCA TGGAAAGCCT CAAGTCTATC
    TGGTCCAGTT CCATGATGCA TCCTGGACCT TCCGCTCTGG AGCAGCTGTT AATGCAGCAG
    AAGCAGAAGC AGCAGCGGGG ACAAGGCACC ATGAACCCTC CACACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013970604.2
    CDS117..3644
    Translation

    Target ORF information:

    RefSeq Version XM_014115129.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014115129.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGAGAAT GTCTTAAGCC ATTCTACCTT ATGAGAAATT GGATGTTCTT GCAGATAGTC 
    ATTGTGGGAG AAAACGTTTC ATATAAGTCC CAGATGAGGA CCGGAAACTT GAAATCAGAG
    GAGCATCTGA AATCAAGTAA TATTAGGCAG GCAGAAGTCC TGAAGGCTGA TATGACAGAT
    TCTAAGCTGG GTCCAGCTGA GGTCTGGACA TCCAGGCAGG CTCTGCAGGA CCTGTACCAG
    AAAATGCTAG TTACTGATTT GGAATACGCT TTAGACAAGA AAGTAGAACA GGATCTCTGG
    AATCATGCCT TTAAGAATCA GATCACAACA CTACAAGGCC AGGCAAAGAA TCGAGCAAAC
    CCGAATCGGA GTGAAGTTCA GGCAAACCTT TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC
    TATACTCAGT TATTACAAGA ACTGTGTACA GTGTTTAATG TAGATTTACC ATGCCGTGTG
    AAGTCTTCCC AATTGGGAAT TATCAGCAAT AAACAGACGC ATACCAGCGC CATAGTGAAG
    CCACAGTCTA GTTCCTGTTC TTATATCTGC CAGCACTGCC TCGTCCACCT TGGAGACATT
    GCTCGATACA GAAACCAGAC CAGCCAGGCA GAGTCCTACT ATAGGCATGC AGCTCAGCTT
    GTCCCCTCTA ATGGTCAGCC TTACAATCAG TTGGCTATCT TAGCTTCTTC CAAAGGAGAC
    CATCTGACCA CAATTTTCTA CTACTGCAGA AGCATCGCTG TGAAGTTCCC TTTCCCAGCT
    GCCTCCACGA ATCTACAAAA AGCACTTTCT AAAGCACTGG AAAGCCGGGA TGAGGTGAAA
    ACCAAATGGG GTGTTTCTGA CTTCATCAAG GCCTTTATTA AATTCCACGG TCATGTGTAC
    CTGAGTAAGA GCTTGGAAAA GCTGAGCCCT CTTCGAGAGA AATTGGAAGA ACAGTTTAAG
    AGGCTGCTAT TCCAAAAAGC TTTCAACTCT CAGCAGTTAG TTCATGTCAC TGTCATTAAC
    CTGTTTCAAC TTCATCACCT TCGTGACTTT AGCAATGAAA CGGAGCAGCA TAGTTATAGC
    CAAGATGAGC AGCTGTGTTG GACACAGTTA CTGGCCCTCT TTATGTCTTT TCTTGGCATC
    CTGTGCAAGT GTCCTCTCCA GAACAAGTCT CAGGAGGAGT CCTACAATGC CTATCCTCTT
    CCTGCAGTCA AGGTCTCCAT GGACTGGCTG AGACTCAGAC CCAGGGTCTT TCAGGAGGCA
    GTGGTAGATG AAAGACAGTA CATTTGGCCC TGGCTAATTT CTCTTCTAAA TAGTTTCCAT
    CCCCATGAAG AGGATCTTTC AAGTACTAAT GCTACACCAC TTCCAGAGGA GTTTGAATTA
    CAAGGATTCT TGGCTTTGAG ACCTTCTTTC AGGAACTTGG ATTTTTCCAA AGGCCATCAG
    GGTATTACAG GAGACAAAGA AGGTCAGCAA CGGCAAATAC GACAGCAGCG CTTGATCTCT
    ATAGGCAAAT GGATTGCCGA TAATCAGCCA AGGCTGATTC AGTGTGAAAA TGAGGTAGGG
    AAATTGTTGT TTATCACAGA AATCCCAGAG TTAACACTAG AAGACCCCAG TGAAGCCAAA
    GAGAACCTCA TTCTGCCAGA AACATCCATG ATAGAGTCAC TATCTGCAGA CGGGAACCCA
    GGACTGAAAT CAGTGCTGTC CACGGGCCGT AATCTAAGCA ACAACTGTGA CCCCGGAGAG
    AAACCTATGG TCACCTTCAA AGAGAACATT AAGCCGCGAG AAGTGAACAG AGACCAAGGA
    AGAAGTTTTC CTCCCAAAGA GGTGAGAAGG GACTATAGCA AAGGAATTAC TATAACTAAG
    AATGATGGAA AGAAGGACAA CAGCAAGAGG AAAACAGAAA CCAAGAAATG CACCTTAGAA
    AAGTTACAGG AAACAGGAAA GCAGAGTGTG GCAGTGCAGG TAAAATCCCA GACAGAATTA
    AGAAAGACTC CAGTGTCTGA AGCCAGGAAA ACCCCTGTAA CTCAAACCCC AAGTCAAGCA
    ACTAACTCCC AGTTCATCCC CATTCATCAC CCTGGAGCCT TCCCTCCTCT TCCCAGCCGG
    CCAGGGTTCC CGCCCCCTAC ATATGTTATT CCCCCTCCTG TGGCATTTTC TATGGGCTCA
    GGTTACACCT TCCCAGCTGG TGTTTCTGTC CCAGGAACCT TTCTTCAGCC TACAGCTCAC
    TCTCCAGCAG GAAACCAGGT GCAAGCTGGG AAACAGTCCC ACATTCCTTA CAGCCAGCAA
    CGGCCCTCTG GACCAGGGCC AATGAACCAG GGACCTCAAC AACCACAGCC ACCTTCCCAG
    CAATCCCTTA CATCTTTACC AGCTCAGCCA ACAGCACAGT CTACAAGCCA GTTGCAGGTT
    CAAGCTCTAG CTCAGCAACA ATCCCCCACA AAAGCTGTGC CAGCTTTGGG GAAAAGCCCT
    CCTCACCACT CTGGATTCCA GCAGTATCAA CAGGCAGATG CCTCCAAACA GCTGTGGAAT
    CCCCCTCAGG TTCAAGGCCC ATTAGGGAAA ATCATGCCTG TGAAACAGCC CTACTACCTT
    CAGTCCCAAG ACCCCATAAA ACTGTTTGAG CCGTCATTGC AACCTCCGGT AATGCAGCAG
    CAGCCTCTAG AGAAAAAAAT GAAGCCTTTT CCCATGGAGC CATATAACCA TAATCCCTCA
    GAAGTCAAGG TCCCAGAATT CTACTGGGAT TCTTCATACA GCATGGCTGA TAACAGAGCT
    GTAATGGCAC AGCAAGCAAA TATGGACCGC AGGGGCAAAC GGTCACCAGG AGTCTTCCGT
    CCAGAGCAGG ATCCTGTGCC CAGGATGCCA TTTGAGAAAT CCTTGTTGGA GAAGCCCTCG
    GAGCTCATGT CCCATTCCTC CTCTTTCTTG TCCCTCACCG GATTCTCTCT CAATCAGGAA
    AGATACCCAA ATAACAGTGT GTTTAATGAG GTATATGGGA AAAACCTGAC AACCAGCTCC
    AAATCAGAAC TTAATCCTTC GCTGGCCCCC CAGGAGACGT CACTCTATTC CCTCTTTGAA
    GGGACCCCCT GGTCTCCATC ACTTCCTGCC AGTTCAGATC ATTCAACACC AGCCAGCCAG
    TCTCCTCATT CCTCTAACCC AAGCAGCCTG CCAAGCTCCC CTCCAACACA CAACCATAAT
    TCTGTTCCAT TCTCCAACTT TGGACCCATT GGGACTCCAG ATAACAGGGA TAGGAGAACC
    GCAGATCGGT GGAAAACCGA TAAGCCAGCC ATGGGTGGGT TTGGCATTGA CTATCTCTCA
    GCAACATCAT CCTCTGAGAG CAGTTGGCAT CAGGCCAGCA CTGCAAGTGG CACCTGGACA
    GGCCACGGCC CCTCCATGGA GGATTCCTCT GCTGTCCTCA TGGAAAGCCT CAAGTCTATC
    TGGTCCAGTT CCATGATGCA TCCTGGACCT TCCGCTCTGG AGCAGCTGTT AATGCAGCAG
    AAGCAGAAGC AGCAGCGGGG ACAAGGCACC ATGAACCCTC CACACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe09147
    Clone ID Related Accession (Same CDS sequence) XM_014115130.2
    Accession Version XM_014115130.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3390bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product protein SMG7 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014115130.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAGAGAAT GTCTTAAGCC ATTCTACCTT ATGAGAAATT GGATGTTCTT GCAGATAGTC 
    ATTGTGGGAG AAAACGTTTC ATATAAGTCC CAGATGAGGA CCGGAAACTT GAAATCAGAG
    GAGCATCTGA AATCAAGTAA TATTAGGCAG GCAGAAGTCC TGAAGGCTGA TATGACAGAT
    TCTAAGCTGG GTCCAGCTGA GGTCTGGACA TCCAGGCAGG CTCTGCAGGA CCTGTACCAG
    AAAATGCTAG TTACTGATTT GGAATACGCT TTAGACAAGA AAGTAGAACA GGATCTCTGG
    AATCATGCCT TTAAGAATCA GATCACAACA CTACAAGGCC AGGCAAAGAA TCGAGCAAAC
    CCGAATCGGA GTGAAGTTCA GGCAAACCTT TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC
    TATACTCAGT TATTACAAGA ACTGTGTACA GTGTTTAATG TAGATTTACC ATGCCGTGTG
    AAGTCTTCCC AATTGGGAAT TATCAGCAAT AAACAGACGC ATACCAGCGC CATAGTGAAG
    CCACAGTCTA GTTCCTGTTC TTATATCTGC CAGCACTGCC TCGTCCACCT TGGAGACATT
    GCTCGATACA GAAACCAGAC CAGCCAGGCA GAGTCCTACT ATAGGCATGC AGCTCAGCTT
    GTCCCCTCTA ATGGTCAGCC TTACAATCAG TTGGCTATCT TAGCTTCTTC CAAAGGAGAC
    CATCTGACCA CAATTTTCTA CTACTGCAGA AGCATCGCTG TGAAGTTCCC TTTCCCAGCT
    GCCTCCACGA ATCTACAAAA AGCACTTTCT AAAGCACTGG AAAGCCGGGA TGAGGTGAAA
    ACCAAATGGG GTGTTTCTGA CTTCATCAAG GCCTTTATTA AATTCCACGG TCATGTGTAC
    CTGAGTAAGA GCTTGGAAAA GCTGAGCCCT CTTCGAGAGA AATTGGAAGA ACAGTTTAAG
    AGGCTGCTAT TCCAAAAAGC TTTCAACTCT CAGCAGTTAG TTCATGTCAC TGTCATTAAC
    CTGTTTCAAC TTCATCACCT TCGTGACTTT AGCAATGAAA CGGAGCAGCA TAGTTATAGC
    CAAGATGAGC AGCTGTGTTG GACACAGTTA CTGGCCCTCT TTATGTCTTT TCTTGGCATC
    CTGTGCAAGT GTCCTCTCCA GAACAAGTCT CAGGAGGAGT CCTACAATGC CTATCCTCTT
    CCTGCAGTCA AGGTCTCCAT GGACTGGCTG AGACTCAGAC CCAGGGTCTT TCAGGAGGCA
    GTGGTAGATG AAAGACAGTA CATTTGGCCC TGGCTAATTT CTCTTCTAAA TAGTTTCCAT
    CCCCATGAAG AGGATCTTTC AAGTACTAAT GCTACACCAC TTCCAGAGGA GTTTGAATTA
    CAAGGATTCT TGGCTTTGAG ACCTTCTTTC AGGAACTTGG ATTTTTCCAA AGGCCATCAG
    GGTATTACAG GAGACAAAGA AGGTCAGCAA CGGCAAATAC GACAGCAGCG CTTGATCTCT
    ATAGGCAAAT GGATTGCCGA TAATCAGCCA AGGCTGATTC AGTGTGAAAA TGAGGTAGGG
    AAATTGTTGT TTATCACAGA AATCCCAGAG TTAACACTAG AAGACCCCAG TGAAGCCAAA
    GAGAACCTCA TTCTGCCAGA AACATCCATG ATAGAGTCAC TATCTGCAGA CGGGAACCCA
    GGACTGAAAT CAGTGCTGTC CACGGGCCGT AATCTAAGCA ACAACTGTGA CCCCGGAGAG
    AAACCTATGG TCACCTTCAA AGAGAACATT AAGCCGCGAG AAGTGAACAG AGACCAAGGA
    AGAAGTTTTC CTCCCAAAGA GGTAAAATCC CAGACAGAAT TAAGAAAGAC TCCAGTGTCT
    GAAGCCAGGA AAACCCCTGT AACTCAAACC CCAAGTCAAG CAACTAACTC CCAGTTCATC
    CCCATTCATC ACCCTGGAGC CTTCCCTCCT CTTCCCAGCC GGCCAGGGTT CCCGCCCCCT
    ACATATGTTA TTCCCCCTCC TGTGGCATTT TCTATGGGCT CAGGTTACAC CTTCCCAGCT
    GGTGTTTCTG TCCCAGGAAC CTTTCTTCAG CCTACAGCTC ACTCTCCAGC AGGAAACCAG
    GTGCAAGCTG GGAAACAGTC CCACATTCCT TACAGCCAGC AACGGCCCTC TGGACCAGGG
    CCAATGAACC AGGGACCTCA ACAACCACAG CCACCTTCCC AGCAATCCCT TACATCTTTA
    CCAGCTCAGC CAACAGCACA GTCTACAAGC CAGTTGCAGG TTCAAGCTCT AGCTCAGCAA
    CAATCCCCCA CAAAAGCTGT GCCAGCTTTG GGGAAAAGCC CTCCTCACCA CTCTGGATTC
    CAGCAGTATC AACAGGCAGA TGCCTCCAAA CAGCTGTGGA ATCCCCCTCA GGTTCAAGGC
    CCATTAGGGA AAATCATGCC TGTGAAACAG CCCTACTACC TTCAGTCCCA AGACCCCATA
    AAACTGTTTG AGCCGTCATT GCAACCTCCG GTAATGCAGC AGCAGCCTCT AGAGAAAAAA
    ATGAAGCCTT TTCCCATGGA GCCATATAAC CATAATCCCT CAGAAGTCAA GGTCCCAGAA
    TTCTACTGGG ATTCTTCATA CAGCATGGCT GATAACAGAG CTGTAATGGC ACAGCAAGCA
    AATATGGACC GCAGGGGCAA ACGGTCACCA GGAGTCTTCC GTCCAGAGCA GGATCCTGTG
    CCCAGGATGC CATTTGAGAA ATCCTTGTTG GAGAAGCCCT CGGAGCTCAT GTCCCATTCC
    TCCTCTTTCT TGTCCCTCAC CGGATTCTCT CTCAATCAGG AAAGATACCC AAATAACAGT
    GTGTTTAATG AGGTATATGG GAAAAACCTG ACAACCAGCT CCAAATCAGA ACTTAATCCT
    TCGCTGGCCC CCCAGGAGAC GTCACTCTAT TCCCTCTTTG AAGGGACCCC CTGGTCTCCA
    TCACTTCCTG CCAGTTCAGA TCATTCAACA CCAGCCAGCC AGTCTCCTCA TTCCTCTAAC
    CCAAGCAGCC TGCCAAGCTC CCCTCCAACA CACAACCATA ATTCTGTTCC ATTCTCCAAC
    TTTGGACCCA TTGGGACTCC AGATAACAGG GATAGGAGAA CCGCAGATCG GTGGAAAACC
    GATAAGCCAG CCATGGGTGG GTTTGGCATT GACTATCTCT CAGCAACATC ATCCTCTGAG
    AGCAGTTGGC ATCAGGCCAG CACTGCAAGT GGCACCTGGA CAGGCCACGG CCCCTCCATG
    GAGGATTCCT CTGCTGTCCT CATGGAAAGC CTCAAGTCTA TCTGGTCCAG TTCCATGATG
    CATCCTGGAC CTTCCGCTCT GGAGCAGCTG TTAATGCAGC AGAAGCAGAA GCAGCAGCGG
    GGACAAGGCA CCATGAACCC TCCACACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013970605.2
    CDS114..3503
    Translation

    Target ORF information:

    RefSeq Version XM_014115130.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014115130.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAGAGAAT GTCTTAAGCC ATTCTACCTT ATGAGAAATT GGATGTTCTT GCAGATAGTC 
    ATTGTGGGAG AAAACGTTTC ATATAAGTCC CAGATGAGGA CCGGAAACTT GAAATCAGAG
    GAGCATCTGA AATCAAGTAA TATTAGGCAG GCAGAAGTCC TGAAGGCTGA TATGACAGAT
    TCTAAGCTGG GTCCAGCTGA GGTCTGGACA TCCAGGCAGG CTCTGCAGGA CCTGTACCAG
    AAAATGCTAG TTACTGATTT GGAATACGCT TTAGACAAGA AAGTAGAACA GGATCTCTGG
    AATCATGCCT TTAAGAATCA GATCACAACA CTACAAGGCC AGGCAAAGAA TCGAGCAAAC
    CCGAATCGGA GTGAAGTTCA GGCAAACCTT TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC
    TATACTCAGT TATTACAAGA ACTGTGTACA GTGTTTAATG TAGATTTACC ATGCCGTGTG
    AAGTCTTCCC AATTGGGAAT TATCAGCAAT AAACAGACGC ATACCAGCGC CATAGTGAAG
    CCACAGTCTA GTTCCTGTTC TTATATCTGC CAGCACTGCC TCGTCCACCT TGGAGACATT
    GCTCGATACA GAAACCAGAC CAGCCAGGCA GAGTCCTACT ATAGGCATGC AGCTCAGCTT
    GTCCCCTCTA ATGGTCAGCC TTACAATCAG TTGGCTATCT TAGCTTCTTC CAAAGGAGAC
    CATCTGACCA CAATTTTCTA CTACTGCAGA AGCATCGCTG TGAAGTTCCC TTTCCCAGCT
    GCCTCCACGA ATCTACAAAA AGCACTTTCT AAAGCACTGG AAAGCCGGGA TGAGGTGAAA
    ACCAAATGGG GTGTTTCTGA CTTCATCAAG GCCTTTATTA AATTCCACGG TCATGTGTAC
    CTGAGTAAGA GCTTGGAAAA GCTGAGCCCT CTTCGAGAGA AATTGGAAGA ACAGTTTAAG
    AGGCTGCTAT TCCAAAAAGC TTTCAACTCT CAGCAGTTAG TTCATGTCAC TGTCATTAAC
    CTGTTTCAAC TTCATCACCT TCGTGACTTT AGCAATGAAA CGGAGCAGCA TAGTTATAGC
    CAAGATGAGC AGCTGTGTTG GACACAGTTA CTGGCCCTCT TTATGTCTTT TCTTGGCATC
    CTGTGCAAGT GTCCTCTCCA GAACAAGTCT CAGGAGGAGT CCTACAATGC CTATCCTCTT
    CCTGCAGTCA AGGTCTCCAT GGACTGGCTG AGACTCAGAC CCAGGGTCTT TCAGGAGGCA
    GTGGTAGATG AAAGACAGTA CATTTGGCCC TGGCTAATTT CTCTTCTAAA TAGTTTCCAT
    CCCCATGAAG AGGATCTTTC AAGTACTAAT GCTACACCAC TTCCAGAGGA GTTTGAATTA
    CAAGGATTCT TGGCTTTGAG ACCTTCTTTC AGGAACTTGG ATTTTTCCAA AGGCCATCAG
    GGTATTACAG GAGACAAAGA AGGTCAGCAA CGGCAAATAC GACAGCAGCG CTTGATCTCT
    ATAGGCAAAT GGATTGCCGA TAATCAGCCA AGGCTGATTC AGTGTGAAAA TGAGGTAGGG
    AAATTGTTGT TTATCACAGA AATCCCAGAG TTAACACTAG AAGACCCCAG TGAAGCCAAA
    GAGAACCTCA TTCTGCCAGA AACATCCATG ATAGAGTCAC TATCTGCAGA CGGGAACCCA
    GGACTGAAAT CAGTGCTGTC CACGGGCCGT AATCTAAGCA ACAACTGTGA CCCCGGAGAG
    AAACCTATGG TCACCTTCAA AGAGAACATT AAGCCGCGAG AAGTGAACAG AGACCAAGGA
    AGAAGTTTTC CTCCCAAAGA GGTAAAATCC CAGACAGAAT TAAGAAAGAC TCCAGTGTCT
    GAAGCCAGGA AAACCCCTGT AACTCAAACC CCAAGTCAAG CAACTAACTC CCAGTTCATC
    CCCATTCATC ACCCTGGAGC CTTCCCTCCT CTTCCCAGCC GGCCAGGGTT CCCGCCCCCT
    ACATATGTTA TTCCCCCTCC TGTGGCATTT TCTATGGGCT CAGGTTACAC CTTCCCAGCT
    GGTGTTTCTG TCCCAGGAAC CTTTCTTCAG CCTACAGCTC ACTCTCCAGC AGGAAACCAG
    GTGCAAGCTG GGAAACAGTC CCACATTCCT TACAGCCAGC AACGGCCCTC TGGACCAGGG
    CCAATGAACC AGGGACCTCA ACAACCACAG CCACCTTCCC AGCAATCCCT TACATCTTTA
    CCAGCTCAGC CAACAGCACA GTCTACAAGC CAGTTGCAGG TTCAAGCTCT AGCTCAGCAA
    CAATCCCCCA CAAAAGCTGT GCCAGCTTTG GGGAAAAGCC CTCCTCACCA CTCTGGATTC
    CAGCAGTATC AACAGGCAGA TGCCTCCAAA CAGCTGTGGA ATCCCCCTCA GGTTCAAGGC
    CCATTAGGGA AAATCATGCC TGTGAAACAG CCCTACTACC TTCAGTCCCA AGACCCCATA
    AAACTGTTTG AGCCGTCATT GCAACCTCCG GTAATGCAGC AGCAGCCTCT AGAGAAAAAA
    ATGAAGCCTT TTCCCATGGA GCCATATAAC CATAATCCCT CAGAAGTCAA GGTCCCAGAA
    TTCTACTGGG ATTCTTCATA CAGCATGGCT GATAACAGAG CTGTAATGGC ACAGCAAGCA
    AATATGGACC GCAGGGGCAA ACGGTCACCA GGAGTCTTCC GTCCAGAGCA GGATCCTGTG
    CCCAGGATGC CATTTGAGAA ATCCTTGTTG GAGAAGCCCT CGGAGCTCAT GTCCCATTCC
    TCCTCTTTCT TGTCCCTCAC CGGATTCTCT CTCAATCAGG AAAGATACCC AAATAACAGT
    GTGTTTAATG AGGTATATGG GAAAAACCTG ACAACCAGCT CCAAATCAGA ACTTAATCCT
    TCGCTGGCCC CCCAGGAGAC GTCACTCTAT TCCCTCTTTG AAGGGACCCC CTGGTCTCCA
    TCACTTCCTG CCAGTTCAGA TCATTCAACA CCAGCCAGCC AGTCTCCTCA TTCCTCTAAC
    CCAAGCAGCC TGCCAAGCTC CCCTCCAACA CACAACCATA ATTCTGTTCC ATTCTCCAAC
    TTTGGACCCA TTGGGACTCC AGATAACAGG GATAGGAGAA CCGCAGATCG GTGGAAAACC
    GATAAGCCAG CCATGGGTGG GTTTGGCATT GACTATCTCT CAGCAACATC ATCCTCTGAG
    AGCAGTTGGC ATCAGGCCAG CACTGCAAGT GGCACCTGGA CAGGCCACGG CCCCTCCATG
    GAGGATTCCT CTGCTGTCCT CATGGAAAGC CTCAAGTCTA TCTGGTCCAG TTCCATGATG
    CATCCTGGAC CTTCCGCTCT GGAGCAGCTG TTAATGCAGC AGAAGCAGAA GCAGCAGCGG
    GGACAAGGCA CCATGAACCC TCCACACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe37419
    Clone ID Related Accession (Same CDS sequence) XM_014115127.1 , XM_014115127.2
    Accession Version XM_014115127.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3648bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product protein SMG7 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726067.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATATAAGTCC 
    CAGATGAGGA CCGGAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG
    GCAGAAGTCC TGAAGGCTGA TATGACAGAT TCTAAGCTGG GTCCAGCTGA GGTCTGGACA
    TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTAG TTACTGATTT GGAATACGCT
    TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCATGCCT TTAAGAATCA GATCACAACA
    CTACAAGGCC AGGCAAAGAA TCGAGCAAAC CCGAATCGGA GTGAAGTTCA GGCAAACCTT
    TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA
    GTGTTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AATTGGGAAT TATCAGCAAT
    AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GTTCCTGTTC TTATATCTGC
    CAGCACTGCC TCGTCCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA
    GAGTCCTACT ATAGGCATGC AGCTCAGCTT GTCCCCTCTA ATGGTCAGCC TTACAATCAG
    TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACCA CAATTTTCTA CTACTGCAGA
    AGCATCGCTG TGAAGTTCCC TTTCCCAGCT GCCTCCACGA ATCTACAAAA AGCACTTTCT
    AAAGCACTGG AAAGCCGGGA TGAGGTGAAA ACCAAATGGG GTGTTTCTGA CTTCATCAAG
    GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GCTGAGCCCT
    CTTCGAGAGA AATTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAAAAAGC TTTCAACTCT
    CAGCAGTTAG TTCATGTCAC TGTCATTAAC CTGTTTCAAC TTCATCACCT TCGTGACTTT
    AGCAATGAAA CGGAGCAGCA TAGTTATAGC CAAGATGAGC AGCTGTGTTG GACACAGTTA
    CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTCCA GAACAAGTCT
    CAGGAGGAGT CCTACAATGC CTATCCTCTT CCTGCAGTCA AGGTCTCCAT GGACTGGCTG
    AGACTCAGAC CCAGGGTCTT TCAGGAGGCA GTGGTAGATG AAAGACAGTA CATTTGGCCC
    TGGCTAATTT CTCTTCTAAA TAGTTTCCAT CCCCATGAAG AGGATCTTTC AAGTACTAAT
    GCTACACCAC TTCCAGAGGA GTTTGAATTA CAAGGATTCT TGGCTTTGAG ACCTTCTTTC
    AGGAACTTGG ATTTTTCCAA AGGCCATCAG GGTATTACAG GAGACAAAGA AGGTCAGCAA
    CGGCAAATAC GACAGCAGCG CTTGATCTCT ATAGGCAAAT GGATTGCCGA TAATCAGCCA
    AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAG
    TTAACACTAG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCCAGA AACATCCATG
    ATAGAGTCAC TATCTGCAGA CGGGAACCCA GGACTGAAAT CAGTGCTGTC CACGGGCCGT
    AATCTAAGCA ACAACTGTGA CCCCGGAGAG AAACCTATGG TCACCTTCAA AGAGAACATT
    AAGCCGCGAG AAGTGAACAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTGAGAAGG
    GACTATAGCA AAGGAATTAC TATAACTAAG AATGATGGAA AGAAGGACAA CAGCAAGAGG
    AAAACAGAAA CCAAGAAATG CACCTTAGAA AAGTTACAGG AAACAGGAAA GCAGAGTGTG
    GCAGTGCAGG TAAAATCCCA GACAGAATTA AGAAAGACTC CAGTGTCTGA AGCCAGGAAA
    ACCCCTGTAA CTCAAACCCC AAGTCAAGCA ACTAACTCCC AGTTCATCCC CATTCATCAC
    CCTGGAGCCT TCCCTCCTCT TCCCAGCCGG CCAGGGTTCC CGCCCCCTAC ATATGTTATT
    CCCCCTCCTG TGGCATTTTC TATGGGCTCA GGTTACACCT TCCCAGCTGG TGTTTCTGTC
    CCAGGAACCT TTCTTCAGCC TACAGCTCAC TCTCCAGCAG GAAACCAGGT GCAAGCTGGG
    AAACAGTCCC ACATTCCTTA CAGCCAGCAA CGGCCCTCTG GACCAGGGCC AATGAACCAG
    GGACCTCAAC AACCACAGCC ACCTTCCCAG CAATCCCTTA CATCTTTACC AGCTCAGCCA
    ACAGCACAGT CTACAAGCCA GTTGCAGGTT CAAGCTCTAG CTCAGCAACA ATCCCCCACA
    AAAGCTGTGC CAGCTTTGGG GAAAAGCCCT CCTCACCACT CTGGATTCCA GCAGTATCAA
    CAGGCAGATG CCTCCAAACA GCTGTGGAAT CCCCCTCAGG TTCAAGGCCC ATTAGGGAAA
    ATCATGCCTG TGAAACAGCC CTACTACCTT CAGTCCCAAG ACCCCATAAA ACTGTTTGAG
    CCGTCATTGC AACCTCCGGT AATGCAGCAG CAGCCTCTAG AGAAAAAAAT GAAGCCTTTT
    CCCATGGAGC CATATAACCA TAATCCCTCA GAAGTCAAGG TCCCAGAATT CTACTGGGAT
    TCTTCATACA GCATGGCTGA TAACAGAGCT GTAATGGCAC AGCAAGCAAA TATGGACCGC
    AGGGGCAAAC GGTCACCAGG AGTCTTCCGT CCAGAGCAGG ATCCTGTGCC CAGGATGCCA
    TTTGAGGACC CCAAGGGCTC CCCTCTGCTT CCTCCGGACC TGTTAAAGAG TCTGGCTGCC
    TTGGAGGAAG AGGAAGAGCT GATTTTTTCT AACCCTCCTG ATCTTTACCC AGCCCTGCTG
    GGGCCTCTCG CCTCTCTTCC TGGACGGAGC CTCTTTAAAT CCTTGTTGGA GAAGCCCTCG
    GAGCTCATGT CCCATTCCTC CTCTTTCTTG TCCCTCACCG GATTCTCTCT CAATCAGGAA
    AGATACCCAA ATAACAGTGT GTTTAATGAG GTATATGGGA AAAACCTGAC AACCAGCTCC
    AAATCAGAAC TTAATCCTTC GCTGGCCCCC CAGGAGACGT CACTCTATTC CCTCTTTGAA
    GGGACCCCCT GGTCTCCATC ACTTCCTGCC AGTTCAGATC ATTCAACACC AGCCAGCCAG
    TCTCCTCATT CCTCTAACCC AAGCAGCCTG CCAAGCTCCC CTCCAACACA CAACCATAAT
    TCTGTTCCAT TCTCCAACTT TGGACCCATT GGGACTCCAG ATAACAGGGA TAGGAGAACC
    GCAGATCGGT GGAAAACCGA TAAGCCAGCC ATGGGTGGGT TTGGCATTGA CTATCTCTCA
    GCAACATCAT CCTCTGAGAG CAGTTGGCAT CAGGCCAGCA CTGCAAGTGG CACCTGGACA
    GGCCACGGCC CCTCCATGGA GGATTCCTCT GCTGTCCTCA TGGAAAGCCT CAAGTCTATC
    TGGTCCAGTT CCATGATGCA TCCTGGACCT TCCGCTCTGG AGCAGCTGTT AATGCAGCAG
    AAGCAGAAGC AGCAGCGGGG ACAAGGCACC ATGAACCCTC CACACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013970602.1
    CDS24..3671
    Translation

    Target ORF information:

    RefSeq Version XM_014115127.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014115127.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATATAAGTCC 
    CAGATGAGGA CCGGAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG
    GCAGAAGTCC TGAAGGCTGA TATGACAGAT TCTAAGCTGG GTCCAGCTGA GGTCTGGACA
    TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTAG TTACTGATTT GGAATACGCT
    TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCATGCCT TTAAGAATCA GATCACAACA
    CTACAAGGCC AGGCAAAGAA TCGAGCAAAC CCGAATCGGA GTGAAGTTCA GGCAAACCTT
    TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA
    GTGTTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AATTGGGAAT TATCAGCAAT
    AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GTTCCTGTTC TTATATCTGC
    CAGCACTGCC TCGTCCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA
    GAGTCCTACT ATAGGCATGC AGCTCAGCTT GTCCCCTCTA ATGGTCAGCC TTACAATCAG
    TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACCA CAATTTTCTA CTACTGCAGA
    AGCATCGCTG TGAAGTTCCC TTTCCCAGCT GCCTCCACGA ATCTACAAAA AGCACTTTCT
    AAAGCACTGG AAAGCCGGGA TGAGGTGAAA ACCAAATGGG GTGTTTCTGA CTTCATCAAG
    GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GCTGAGCCCT
    CTTCGAGAGA AATTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAAAAAGC TTTCAACTCT
    CAGCAGTTAG TTCATGTCAC TGTCATTAAC CTGTTTCAAC TTCATCACCT TCGTGACTTT
    AGCAATGAAA CGGAGCAGCA TAGTTATAGC CAAGATGAGC AGCTGTGTTG GACACAGTTA
    CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTCCA GAACAAGTCT
    CAGGAGGAGT CCTACAATGC CTATCCTCTT CCTGCAGTCA AGGTCTCCAT GGACTGGCTG
    AGACTCAGAC CCAGGGTCTT TCAGGAGGCA GTGGTAGATG AAAGACAGTA CATTTGGCCC
    TGGCTAATTT CTCTTCTAAA TAGTTTCCAT CCCCATGAAG AGGATCTTTC AAGTACTAAT
    GCTACACCAC TTCCAGAGGA GTTTGAATTA CAAGGATTCT TGGCTTTGAG ACCTTCTTTC
    AGGAACTTGG ATTTTTCCAA AGGCCATCAG GGTATTACAG GAGACAAAGA AGGTCAGCAA
    CGGCAAATAC GACAGCAGCG CTTGATCTCT ATAGGCAAAT GGATTGCCGA TAATCAGCCA
    AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAG
    TTAACACTAG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCCAGA AACATCCATG
    ATAGAGTCAC TATCTGCAGA CGGGAACCCA GGACTGAAAT CAGTGCTGTC CACGGGCCGT
    AATCTAAGCA ACAACTGTGA CCCCGGAGAG AAACCTATGG TCACCTTCAA AGAGAACATT
    AAGCCGCGAG AAGTGAACAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTGAGAAGG
    GACTATAGCA AAGGAATTAC TATAACTAAG AATGATGGAA AGAAGGACAA CAGCAAGAGG
    AAAACAGAAA CCAAGAAATG CACCTTAGAA AAGTTACAGG AAACAGGAAA GCAGAGTGTG
    GCAGTGCAGG TAAAATCCCA GACAGAATTA AGAAAGACTC CAGTGTCTGA AGCCAGGAAA
    ACCCCTGTAA CTCAAACCCC AAGTCAAGCA ACTAACTCCC AGTTCATCCC CATTCATCAC
    CCTGGAGCCT TCCCTCCTCT TCCCAGCCGG CCAGGGTTCC CGCCCCCTAC ATATGTTATT
    CCCCCTCCTG TGGCATTTTC TATGGGCTCA GGTTACACCT TCCCAGCTGG TGTTTCTGTC
    CCAGGAACCT TTCTTCAGCC TACAGCTCAC TCTCCAGCAG GAAACCAGGT GCAAGCTGGG
    AAACAGTCCC ACATTCCTTA CAGCCAGCAA CGGCCCTCTG GACCAGGGCC AATGAACCAG
    GGACCTCAAC AACCACAGCC ACCTTCCCAG CAATCCCTTA CATCTTTACC AGCTCAGCCA
    ACAGCACAGT CTACAAGCCA GTTGCAGGTT CAAGCTCTAG CTCAGCAACA ATCCCCCACA
    AAAGCTGTGC CAGCTTTGGG GAAAAGCCCT CCTCACCACT CTGGATTCCA GCAGTATCAA
    CAGGCAGATG CCTCCAAACA GCTGTGGAAT CCCCCTCAGG TTCAAGGCCC ATTAGGGAAA
    ATCATGCCTG TGAAACAGCC CTACTACCTT CAGTCCCAAG ACCCCATAAA ACTGTTTGAG
    CCGTCATTGC AACCTCCGGT AATGCAGCAG CAGCCTCTAG AGAAAAAAAT GAAGCCTTTT
    CCCATGGAGC CATATAACCA TAATCCCTCA GAAGTCAAGG TCCCAGAATT CTACTGGGAT
    TCTTCATACA GCATGGCTGA TAACAGAGCT GTAATGGCAC AGCAAGCAAA TATGGACCGC
    AGGGGCAAAC GGTCACCAGG AGTCTTCCGT CCAGAGCAGG ATCCTGTGCC CAGGATGCCA
    TTTGAGGACC CCAAGGGCTC CCCTCTGCTT CCTCCGGACC TGTTAAAGAG TCTGGCTGCC
    TTGGAGGAAG AGGAAGAGCT GATTTTTTCT AACCCTCCTG ATCTTTACCC AGCCCTGCTG
    GGGCCTCTCG CCTCTCTTCC TGGACGGAGC CTCTTTAAAT CCTTGTTGGA GAAGCCCTCG
    GAGCTCATGT CCCATTCCTC CTCTTTCTTG TCCCTCACCG GATTCTCTCT CAATCAGGAA
    AGATACCCAA ATAACAGTGT GTTTAATGAG GTATATGGGA AAAACCTGAC AACCAGCTCC
    AAATCAGAAC TTAATCCTTC GCTGGCCCCC CAGGAGACGT CACTCTATTC CCTCTTTGAA
    GGGACCCCCT GGTCTCCATC ACTTCCTGCC AGTTCAGATC ATTCAACACC AGCCAGCCAG
    TCTCCTCATT CCTCTAACCC AAGCAGCCTG CCAAGCTCCC CTCCAACACA CAACCATAAT
    TCTGTTCCAT TCTCCAACTT TGGACCCATT GGGACTCCAG ATAACAGGGA TAGGAGAACC
    GCAGATCGGT GGAAAACCGA TAAGCCAGCC ATGGGTGGGT TTGGCATTGA CTATCTCTCA
    GCAACATCAT CCTCTGAGAG CAGTTGGCAT CAGGCCAGCA CTGCAAGTGG CACCTGGACA
    GGCCACGGCC CCTCCATGGA GGATTCCTCT GCTGTCCTCA TGGAAAGCCT CAAGTCTATC
    TGGTCCAGTT CCATGATGCA TCCTGGACCT TCCGCTCTGG AGCAGCTGTT AATGCAGCAG
    AAGCAGAAGC AGCAGCGGGG ACAAGGCACC ATGAACCCTC CACACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe37419
    Clone ID Related Accession (Same CDS sequence) XM_014115127.1 , XM_014115127.2
    Accession Version XM_014115127.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3648bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product protein SMG7 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014115127.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATATAAGTCC 
    CAGATGAGGA CCGGAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG
    GCAGAAGTCC TGAAGGCTGA TATGACAGAT TCTAAGCTGG GTCCAGCTGA GGTCTGGACA
    TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTAG TTACTGATTT GGAATACGCT
    TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCATGCCT TTAAGAATCA GATCACAACA
    CTACAAGGCC AGGCAAAGAA TCGAGCAAAC CCGAATCGGA GTGAAGTTCA GGCAAACCTT
    TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA
    GTGTTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AATTGGGAAT TATCAGCAAT
    AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GTTCCTGTTC TTATATCTGC
    CAGCACTGCC TCGTCCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA
    GAGTCCTACT ATAGGCATGC AGCTCAGCTT GTCCCCTCTA ATGGTCAGCC TTACAATCAG
    TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACCA CAATTTTCTA CTACTGCAGA
    AGCATCGCTG TGAAGTTCCC TTTCCCAGCT GCCTCCACGA ATCTACAAAA AGCACTTTCT
    AAAGCACTGG AAAGCCGGGA TGAGGTGAAA ACCAAATGGG GTGTTTCTGA CTTCATCAAG
    GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GCTGAGCCCT
    CTTCGAGAGA AATTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAAAAAGC TTTCAACTCT
    CAGCAGTTAG TTCATGTCAC TGTCATTAAC CTGTTTCAAC TTCATCACCT TCGTGACTTT
    AGCAATGAAA CGGAGCAGCA TAGTTATAGC CAAGATGAGC AGCTGTGTTG GACACAGTTA
    CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTCCA GAACAAGTCT
    CAGGAGGAGT CCTACAATGC CTATCCTCTT CCTGCAGTCA AGGTCTCCAT GGACTGGCTG
    AGACTCAGAC CCAGGGTCTT TCAGGAGGCA GTGGTAGATG AAAGACAGTA CATTTGGCCC
    TGGCTAATTT CTCTTCTAAA TAGTTTCCAT CCCCATGAAG AGGATCTTTC AAGTACTAAT
    GCTACACCAC TTCCAGAGGA GTTTGAATTA CAAGGATTCT TGGCTTTGAG ACCTTCTTTC
    AGGAACTTGG ATTTTTCCAA AGGCCATCAG GGTATTACAG GAGACAAAGA AGGTCAGCAA
    CGGCAAATAC GACAGCAGCG CTTGATCTCT ATAGGCAAAT GGATTGCCGA TAATCAGCCA
    AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAG
    TTAACACTAG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCCAGA AACATCCATG
    ATAGAGTCAC TATCTGCAGA CGGGAACCCA GGACTGAAAT CAGTGCTGTC CACGGGCCGT
    AATCTAAGCA ACAACTGTGA CCCCGGAGAG AAACCTATGG TCACCTTCAA AGAGAACATT
    AAGCCGCGAG AAGTGAACAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTGAGAAGG
    GACTATAGCA AAGGAATTAC TATAACTAAG AATGATGGAA AGAAGGACAA CAGCAAGAGG
    AAAACAGAAA CCAAGAAATG CACCTTAGAA AAGTTACAGG AAACAGGAAA GCAGAGTGTG
    GCAGTGCAGG TAAAATCCCA GACAGAATTA AGAAAGACTC CAGTGTCTGA AGCCAGGAAA
    ACCCCTGTAA CTCAAACCCC AAGTCAAGCA ACTAACTCCC AGTTCATCCC CATTCATCAC
    CCTGGAGCCT TCCCTCCTCT TCCCAGCCGG CCAGGGTTCC CGCCCCCTAC ATATGTTATT
    CCCCCTCCTG TGGCATTTTC TATGGGCTCA GGTTACACCT TCCCAGCTGG TGTTTCTGTC
    CCAGGAACCT TTCTTCAGCC TACAGCTCAC TCTCCAGCAG GAAACCAGGT GCAAGCTGGG
    AAACAGTCCC ACATTCCTTA CAGCCAGCAA CGGCCCTCTG GACCAGGGCC AATGAACCAG
    GGACCTCAAC AACCACAGCC ACCTTCCCAG CAATCCCTTA CATCTTTACC AGCTCAGCCA
    ACAGCACAGT CTACAAGCCA GTTGCAGGTT CAAGCTCTAG CTCAGCAACA ATCCCCCACA
    AAAGCTGTGC CAGCTTTGGG GAAAAGCCCT CCTCACCACT CTGGATTCCA GCAGTATCAA
    CAGGCAGATG CCTCCAAACA GCTGTGGAAT CCCCCTCAGG TTCAAGGCCC ATTAGGGAAA
    ATCATGCCTG TGAAACAGCC CTACTACCTT CAGTCCCAAG ACCCCATAAA ACTGTTTGAG
    CCGTCATTGC AACCTCCGGT AATGCAGCAG CAGCCTCTAG AGAAAAAAAT GAAGCCTTTT
    CCCATGGAGC CATATAACCA TAATCCCTCA GAAGTCAAGG TCCCAGAATT CTACTGGGAT
    TCTTCATACA GCATGGCTGA TAACAGAGCT GTAATGGCAC AGCAAGCAAA TATGGACCGC
    AGGGGCAAAC GGTCACCAGG AGTCTTCCGT CCAGAGCAGG ATCCTGTGCC CAGGATGCCA
    TTTGAGGACC CCAAGGGCTC CCCTCTGCTT CCTCCGGACC TGTTAAAGAG TCTGGCTGCC
    TTGGAGGAAG AGGAAGAGCT GATTTTTTCT AACCCTCCTG ATCTTTACCC AGCCCTGCTG
    GGGCCTCTCG CCTCTCTTCC TGGACGGAGC CTCTTTAAAT CCTTGTTGGA GAAGCCCTCG
    GAGCTCATGT CCCATTCCTC CTCTTTCTTG TCCCTCACCG GATTCTCTCT CAATCAGGAA
    AGATACCCAA ATAACAGTGT GTTTAATGAG GTATATGGGA AAAACCTGAC AACCAGCTCC
    AAATCAGAAC TTAATCCTTC GCTGGCCCCC CAGGAGACGT CACTCTATTC CCTCTTTGAA
    GGGACCCCCT GGTCTCCATC ACTTCCTGCC AGTTCAGATC ATTCAACACC AGCCAGCCAG
    TCTCCTCATT CCTCTAACCC AAGCAGCCTG CCAAGCTCCC CTCCAACACA CAACCATAAT
    TCTGTTCCAT TCTCCAACTT TGGACCCATT GGGACTCCAG ATAACAGGGA TAGGAGAACC
    GCAGATCGGT GGAAAACCGA TAAGCCAGCC ATGGGTGGGT TTGGCATTGA CTATCTCTCA
    GCAACATCAT CCTCTGAGAG CAGTTGGCAT CAGGCCAGCA CTGCAAGTGG CACCTGGACA
    GGCCACGGCC CCTCCATGGA GGATTCCTCT GCTGTCCTCA TGGAAAGCCT CAAGTCTATC
    TGGTCCAGTT CCATGATGCA TCCTGGACCT TCCGCTCTGG AGCAGCTGTT AATGCAGCAG
    AAGCAGAAGC AGCAGCGGGG ACAAGGCACC ATGAACCCTC CACACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013970602.1
    CDS263..3910
    Translation

    Target ORF information:

    RefSeq Version XM_014115127.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014115127.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATATAAGTCC 
    CAGATGAGGA CCGGAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG
    GCAGAAGTCC TGAAGGCTGA TATGACAGAT TCTAAGCTGG GTCCAGCTGA GGTCTGGACA
    TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTAG TTACTGATTT GGAATACGCT
    TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCATGCCT TTAAGAATCA GATCACAACA
    CTACAAGGCC AGGCAAAGAA TCGAGCAAAC CCGAATCGGA GTGAAGTTCA GGCAAACCTT
    TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA
    GTGTTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AATTGGGAAT TATCAGCAAT
    AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GTTCCTGTTC TTATATCTGC
    CAGCACTGCC TCGTCCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA
    GAGTCCTACT ATAGGCATGC AGCTCAGCTT GTCCCCTCTA ATGGTCAGCC TTACAATCAG
    TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACCA CAATTTTCTA CTACTGCAGA
    AGCATCGCTG TGAAGTTCCC TTTCCCAGCT GCCTCCACGA ATCTACAAAA AGCACTTTCT
    AAAGCACTGG AAAGCCGGGA TGAGGTGAAA ACCAAATGGG GTGTTTCTGA CTTCATCAAG
    GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GCTGAGCCCT
    CTTCGAGAGA AATTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAAAAAGC TTTCAACTCT
    CAGCAGTTAG TTCATGTCAC TGTCATTAAC CTGTTTCAAC TTCATCACCT TCGTGACTTT
    AGCAATGAAA CGGAGCAGCA TAGTTATAGC CAAGATGAGC AGCTGTGTTG GACACAGTTA
    CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTCCA GAACAAGTCT
    CAGGAGGAGT CCTACAATGC CTATCCTCTT CCTGCAGTCA AGGTCTCCAT GGACTGGCTG
    AGACTCAGAC CCAGGGTCTT TCAGGAGGCA GTGGTAGATG AAAGACAGTA CATTTGGCCC
    TGGCTAATTT CTCTTCTAAA TAGTTTCCAT CCCCATGAAG AGGATCTTTC AAGTACTAAT
    GCTACACCAC TTCCAGAGGA GTTTGAATTA CAAGGATTCT TGGCTTTGAG ACCTTCTTTC
    AGGAACTTGG ATTTTTCCAA AGGCCATCAG GGTATTACAG GAGACAAAGA AGGTCAGCAA
    CGGCAAATAC GACAGCAGCG CTTGATCTCT ATAGGCAAAT GGATTGCCGA TAATCAGCCA
    AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAG
    TTAACACTAG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCCAGA AACATCCATG
    ATAGAGTCAC TATCTGCAGA CGGGAACCCA GGACTGAAAT CAGTGCTGTC CACGGGCCGT
    AATCTAAGCA ACAACTGTGA CCCCGGAGAG AAACCTATGG TCACCTTCAA AGAGAACATT
    AAGCCGCGAG AAGTGAACAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTGAGAAGG
    GACTATAGCA AAGGAATTAC TATAACTAAG AATGATGGAA AGAAGGACAA CAGCAAGAGG
    AAAACAGAAA CCAAGAAATG CACCTTAGAA AAGTTACAGG AAACAGGAAA GCAGAGTGTG
    GCAGTGCAGG TAAAATCCCA GACAGAATTA AGAAAGACTC CAGTGTCTGA AGCCAGGAAA
    ACCCCTGTAA CTCAAACCCC AAGTCAAGCA ACTAACTCCC AGTTCATCCC CATTCATCAC
    CCTGGAGCCT TCCCTCCTCT TCCCAGCCGG CCAGGGTTCC CGCCCCCTAC ATATGTTATT
    CCCCCTCCTG TGGCATTTTC TATGGGCTCA GGTTACACCT TCCCAGCTGG TGTTTCTGTC
    CCAGGAACCT TTCTTCAGCC TACAGCTCAC TCTCCAGCAG GAAACCAGGT GCAAGCTGGG
    AAACAGTCCC ACATTCCTTA CAGCCAGCAA CGGCCCTCTG GACCAGGGCC AATGAACCAG
    GGACCTCAAC AACCACAGCC ACCTTCCCAG CAATCCCTTA CATCTTTACC AGCTCAGCCA
    ACAGCACAGT CTACAAGCCA GTTGCAGGTT CAAGCTCTAG CTCAGCAACA ATCCCCCACA
    AAAGCTGTGC CAGCTTTGGG GAAAAGCCCT CCTCACCACT CTGGATTCCA GCAGTATCAA
    CAGGCAGATG CCTCCAAACA GCTGTGGAAT CCCCCTCAGG TTCAAGGCCC ATTAGGGAAA
    ATCATGCCTG TGAAACAGCC CTACTACCTT CAGTCCCAAG ACCCCATAAA ACTGTTTGAG
    CCGTCATTGC AACCTCCGGT AATGCAGCAG CAGCCTCTAG AGAAAAAAAT GAAGCCTTTT
    CCCATGGAGC CATATAACCA TAATCCCTCA GAAGTCAAGG TCCCAGAATT CTACTGGGAT
    TCTTCATACA GCATGGCTGA TAACAGAGCT GTAATGGCAC AGCAAGCAAA TATGGACCGC
    AGGGGCAAAC GGTCACCAGG AGTCTTCCGT CCAGAGCAGG ATCCTGTGCC CAGGATGCCA
    TTTGAGGACC CCAAGGGCTC CCCTCTGCTT CCTCCGGACC TGTTAAAGAG TCTGGCTGCC
    TTGGAGGAAG AGGAAGAGCT GATTTTTTCT AACCCTCCTG ATCTTTACCC AGCCCTGCTG
    GGGCCTCTCG CCTCTCTTCC TGGACGGAGC CTCTTTAAAT CCTTGTTGGA GAAGCCCTCG
    GAGCTCATGT CCCATTCCTC CTCTTTCTTG TCCCTCACCG GATTCTCTCT CAATCAGGAA
    AGATACCCAA ATAACAGTGT GTTTAATGAG GTATATGGGA AAAACCTGAC AACCAGCTCC
    AAATCAGAAC TTAATCCTTC GCTGGCCCCC CAGGAGACGT CACTCTATTC CCTCTTTGAA
    GGGACCCCCT GGTCTCCATC ACTTCCTGCC AGTTCAGATC ATTCAACACC AGCCAGCCAG
    TCTCCTCATT CCTCTAACCC AAGCAGCCTG CCAAGCTCCC CTCCAACACA CAACCATAAT
    TCTGTTCCAT TCTCCAACTT TGGACCCATT GGGACTCCAG ATAACAGGGA TAGGAGAACC
    GCAGATCGGT GGAAAACCGA TAAGCCAGCC ATGGGTGGGT TTGGCATTGA CTATCTCTCA
    GCAACATCAT CCTCTGAGAG CAGTTGGCAT CAGGCCAGCA CTGCAAGTGG CACCTGGACA
    GGCCACGGCC CCTCCATGGA GGATTCCTCT GCTGTCCTCA TGGAAAGCCT CAAGTCTATC
    TGGTCCAGTT CCATGATGCA TCCTGGACCT TCCGCTCTGG AGCAGCTGTT AATGCAGCAG
    AAGCAGAAGC AGCAGCGGGG ACAAGGCACC ATGAACCCTC CACACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe37420
    Clone ID Related Accession (Same CDS sequence) XM_014115128.1
    Accession Version XM_014115128.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3510bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product protein SMG7 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726067.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATATAAGTCC 
    CAGATGAGGA CCGGAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG
    GCAGAAGTCC TGAAGGCTGA TATGACAGAT TCTAAGCTGG GTCCAGCTGA GGTCTGGACA
    TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTAG TTACTGATTT GGAATACGCT
    TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCATGCCT TTAAGAATCA GATCACAACA
    CTACAAGGCC AGGCAAAGAA TCGAGCAAAC CCGAATCGGA GTGAAGTTCA GGCAAACCTT
    TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA
    GTGTTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AATTGGGAAT TATCAGCAAT
    AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GTTCCTGTTC TTATATCTGC
    CAGCACTGCC TCGTCCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA
    GAGTCCTACT ATAGGCATGC AGCTCAGCTT GTCCCCTCTA ATGGTCAGCC TTACAATCAG
    TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACCA CAATTTTCTA CTACTGCAGA
    AGCATCGCTG TGAAGTTCCC TTTCCCAGCT GCCTCCACGA ATCTACAAAA AGCACTTTCT
    AAAGCACTGG AAAGCCGGGA TGAGGTGAAA ACCAAATGGG GTGTTTCTGA CTTCATCAAG
    GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GCTGAGCCCT
    CTTCGAGAGA AATTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAAAAAGC TTTCAACTCT
    CAGCAGTTAG TTCATGTCAC TGTCATTAAC CTGTTTCAAC TTCATCACCT TCGTGACTTT
    AGCAATGAAA CGGAGCAGCA TAGTTATAGC CAAGATGAGC AGCTGTGTTG GACACAGTTA
    CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTCCA GAACAAGTCT
    CAGGAGGAGT CCTACAATGC CTATCCTCTT CCTGCAGTCA AGGTCTCCAT GGACTGGCTG
    AGACTCAGAC CCAGGGTCTT TCAGGAGGCA GTGGTAGATG AAAGACAGTA CATTTGGCCC
    TGGCTAATTT CTCTTCTAAA TAGTTTCCAT CCCCATGAAG AGGATCTTTC AAGTACTAAT
    GCTACACCAC TTCCAGAGGA GTTTGAATTA CAAGGATTCT TGGCTTTGAG ACCTTCTTTC
    AGGAACTTGG ATTTTTCCAA AGGCCATCAG GGTATTACAG GAGACAAAGA AGGTCAGCAA
    CGGCAAATAC GACAGCAGCG CTTGATCTCT ATAGGCAAAT GGATTGCCGA TAATCAGCCA
    AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAG
    TTAACACTAG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCCAGA AACATCCATG
    ATAGAGTCAC TATCTGCAGA CGGGAACCCA GGACTGAAAT CAGTGCTGTC CACGGGCCGT
    AATCTAAGCA ACAACTGTGA CCCCGGAGAG AAACCTATGG TCACCTTCAA AGAGAACATT
    AAGCCGCGAG AAGTGAACAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTAAAATCC
    CAGACAGAAT TAAGAAAGAC TCCAGTGTCT GAAGCCAGGA AAACCCCTGT AACTCAAACC
    CCAAGTCAAG CAACTAACTC CCAGTTCATC CCCATTCATC ACCCTGGAGC CTTCCCTCCT
    CTTCCCAGCC GGCCAGGGTT CCCGCCCCCT ACATATGTTA TTCCCCCTCC TGTGGCATTT
    TCTATGGGCT CAGGTTACAC CTTCCCAGCT GGTGTTTCTG TCCCAGGAAC CTTTCTTCAG
    CCTACAGCTC ACTCTCCAGC AGGAAACCAG GTGCAAGCTG GGAAACAGTC CCACATTCCT
    TACAGCCAGC AACGGCCCTC TGGACCAGGG CCAATGAACC AGGGACCTCA ACAACCACAG
    CCACCTTCCC AGCAATCCCT TACATCTTTA CCAGCTCAGC CAACAGCACA GTCTACAAGC
    CAGTTGCAGG TTCAAGCTCT AGCTCAGCAA CAATCCCCCA CAAAAGCTGT GCCAGCTTTG
    GGGAAAAGCC CTCCTCACCA CTCTGGATTC CAGCAGTATC AACAGGCAGA TGCCTCCAAA
    CAGCTGTGGA ATCCCCCTCA GGTTCAAGGC CCATTAGGGA AAATCATGCC TGTGAAACAG
    CCCTACTACC TTCAGTCCCA AGACCCCATA AAACTGTTTG AGCCGTCATT GCAACCTCCG
    GTAATGCAGC AGCAGCCTCT AGAGAAAAAA ATGAAGCCTT TTCCCATGGA GCCATATAAC
    CATAATCCCT CAGAAGTCAA GGTCCCAGAA TTCTACTGGG ATTCTTCATA CAGCATGGCT
    GATAACAGAG CTGTAATGGC ACAGCAAGCA AATATGGACC GCAGGGGCAA ACGGTCACCA
    GGAGTCTTCC GTCCAGAGCA GGATCCTGTG CCCAGGATGC CATTTGAGGA CCCCAAGGGC
    TCCCCTCTGC TTCCTCCGGA CCTGTTAAAG AGTCTGGCTG CCTTGGAGGA AGAGGAAGAG
    CTGATTTTTT CTAACCCTCC TGATCTTTAC CCAGCCCTGC TGGGGCCTCT CGCCTCTCTT
    CCTGGACGGA GCCTCTTTAA ATCCTTGTTG GAGAAGCCCT CGGAGCTCAT GTCCCATTCC
    TCCTCTTTCT TGTCCCTCAC CGGATTCTCT CTCAATCAGG AAAGATACCC AAATAACAGT
    GTGTTTAATG AGGTATATGG GAAAAACCTG ACAACCAGCT CCAAATCAGA ACTTAATCCT
    TCGCTGGCCC CCCAGGAGAC GTCACTCTAT TCCCTCTTTG AAGGGACCCC CTGGTCTCCA
    TCACTTCCTG CCAGTTCAGA TCATTCAACA CCAGCCAGCC AGTCTCCTCA TTCCTCTAAC
    CCAAGCAGCC TGCCAAGCTC CCCTCCAACA CACAACCATA ATTCTGTTCC ATTCTCCAAC
    TTTGGACCCA TTGGGACTCC AGATAACAGG GATAGGAGAA CCGCAGATCG GTGGAAAACC
    GATAAGCCAG CCATGGGTGG GTTTGGCATT GACTATCTCT CAGCAACATC ATCCTCTGAG
    AGCAGTTGGC ATCAGGCCAG CACTGCAAGT GGCACCTGGA CAGGCCACGG CCCCTCCATG
    GAGGATTCCT CTGCTGTCCT CATGGAAAGC CTCAAGTCTA TCTGGTCCAG TTCCATGATG
    CATCCTGGAC CTTCCGCTCT GGAGCAGCTG TTAATGCAGC AGAAGCAGAA GCAGCAGCGG
    GGACAAGGCA CCATGAACCC TCCACACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013970603.1
    CDS24..3533
    Translation

    Target ORF information:

    RefSeq Version XM_014115128.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014115128.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATATAAGTCC 
    CAGATGAGGA CCGGAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG
    GCAGAAGTCC TGAAGGCTGA TATGACAGAT TCTAAGCTGG GTCCAGCTGA GGTCTGGACA
    TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTAG TTACTGATTT GGAATACGCT
    TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCATGCCT TTAAGAATCA GATCACAACA
    CTACAAGGCC AGGCAAAGAA TCGAGCAAAC CCGAATCGGA GTGAAGTTCA GGCAAACCTT
    TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA
    GTGTTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AATTGGGAAT TATCAGCAAT
    AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GTTCCTGTTC TTATATCTGC
    CAGCACTGCC TCGTCCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA
    GAGTCCTACT ATAGGCATGC AGCTCAGCTT GTCCCCTCTA ATGGTCAGCC TTACAATCAG
    TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACCA CAATTTTCTA CTACTGCAGA
    AGCATCGCTG TGAAGTTCCC TTTCCCAGCT GCCTCCACGA ATCTACAAAA AGCACTTTCT
    AAAGCACTGG AAAGCCGGGA TGAGGTGAAA ACCAAATGGG GTGTTTCTGA CTTCATCAAG
    GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GCTGAGCCCT
    CTTCGAGAGA AATTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAAAAAGC TTTCAACTCT
    CAGCAGTTAG TTCATGTCAC TGTCATTAAC CTGTTTCAAC TTCATCACCT TCGTGACTTT
    AGCAATGAAA CGGAGCAGCA TAGTTATAGC CAAGATGAGC AGCTGTGTTG GACACAGTTA
    CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTCCA GAACAAGTCT
    CAGGAGGAGT CCTACAATGC CTATCCTCTT CCTGCAGTCA AGGTCTCCAT GGACTGGCTG
    AGACTCAGAC CCAGGGTCTT TCAGGAGGCA GTGGTAGATG AAAGACAGTA CATTTGGCCC
    TGGCTAATTT CTCTTCTAAA TAGTTTCCAT CCCCATGAAG AGGATCTTTC AAGTACTAAT
    GCTACACCAC TTCCAGAGGA GTTTGAATTA CAAGGATTCT TGGCTTTGAG ACCTTCTTTC
    AGGAACTTGG ATTTTTCCAA AGGCCATCAG GGTATTACAG GAGACAAAGA AGGTCAGCAA
    CGGCAAATAC GACAGCAGCG CTTGATCTCT ATAGGCAAAT GGATTGCCGA TAATCAGCCA
    AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAG
    TTAACACTAG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCCAGA AACATCCATG
    ATAGAGTCAC TATCTGCAGA CGGGAACCCA GGACTGAAAT CAGTGCTGTC CACGGGCCGT
    AATCTAAGCA ACAACTGTGA CCCCGGAGAG AAACCTATGG TCACCTTCAA AGAGAACATT
    AAGCCGCGAG AAGTGAACAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTAAAATCC
    CAGACAGAAT TAAGAAAGAC TCCAGTGTCT GAAGCCAGGA AAACCCCTGT AACTCAAACC
    CCAAGTCAAG CAACTAACTC CCAGTTCATC CCCATTCATC ACCCTGGAGC CTTCCCTCCT
    CTTCCCAGCC GGCCAGGGTT CCCGCCCCCT ACATATGTTA TTCCCCCTCC TGTGGCATTT
    TCTATGGGCT CAGGTTACAC CTTCCCAGCT GGTGTTTCTG TCCCAGGAAC CTTTCTTCAG
    CCTACAGCTC ACTCTCCAGC AGGAAACCAG GTGCAAGCTG GGAAACAGTC CCACATTCCT
    TACAGCCAGC AACGGCCCTC TGGACCAGGG CCAATGAACC AGGGACCTCA ACAACCACAG
    CCACCTTCCC AGCAATCCCT TACATCTTTA CCAGCTCAGC CAACAGCACA GTCTACAAGC
    CAGTTGCAGG TTCAAGCTCT AGCTCAGCAA CAATCCCCCA CAAAAGCTGT GCCAGCTTTG
    GGGAAAAGCC CTCCTCACCA CTCTGGATTC CAGCAGTATC AACAGGCAGA TGCCTCCAAA
    CAGCTGTGGA ATCCCCCTCA GGTTCAAGGC CCATTAGGGA AAATCATGCC TGTGAAACAG
    CCCTACTACC TTCAGTCCCA AGACCCCATA AAACTGTTTG AGCCGTCATT GCAACCTCCG
    GTAATGCAGC AGCAGCCTCT AGAGAAAAAA ATGAAGCCTT TTCCCATGGA GCCATATAAC
    CATAATCCCT CAGAAGTCAA GGTCCCAGAA TTCTACTGGG ATTCTTCATA CAGCATGGCT
    GATAACAGAG CTGTAATGGC ACAGCAAGCA AATATGGACC GCAGGGGCAA ACGGTCACCA
    GGAGTCTTCC GTCCAGAGCA GGATCCTGTG CCCAGGATGC CATTTGAGGA CCCCAAGGGC
    TCCCCTCTGC TTCCTCCGGA CCTGTTAAAG AGTCTGGCTG CCTTGGAGGA AGAGGAAGAG
    CTGATTTTTT CTAACCCTCC TGATCTTTAC CCAGCCCTGC TGGGGCCTCT CGCCTCTCTT
    CCTGGACGGA GCCTCTTTAA ATCCTTGTTG GAGAAGCCCT CGGAGCTCAT GTCCCATTCC
    TCCTCTTTCT TGTCCCTCAC CGGATTCTCT CTCAATCAGG AAAGATACCC AAATAACAGT
    GTGTTTAATG AGGTATATGG GAAAAACCTG ACAACCAGCT CCAAATCAGA ACTTAATCCT
    TCGCTGGCCC CCCAGGAGAC GTCACTCTAT TCCCTCTTTG AAGGGACCCC CTGGTCTCCA
    TCACTTCCTG CCAGTTCAGA TCATTCAACA CCAGCCAGCC AGTCTCCTCA TTCCTCTAAC
    CCAAGCAGCC TGCCAAGCTC CCCTCCAACA CACAACCATA ATTCTGTTCC ATTCTCCAAC
    TTTGGACCCA TTGGGACTCC AGATAACAGG GATAGGAGAA CCGCAGATCG GTGGAAAACC
    GATAAGCCAG CCATGGGTGG GTTTGGCATT GACTATCTCT CAGCAACATC ATCCTCTGAG
    AGCAGTTGGC ATCAGGCCAG CACTGCAAGT GGCACCTGGA CAGGCCACGG CCCCTCCATG
    GAGGATTCCT CTGCTGTCCT CATGGAAAGC CTCAAGTCTA TCTGGTCCAG TTCCATGATG
    CATCCTGGAC CTTCCGCTCT GGAGCAGCTG TTAATGCAGC AGAAGCAGAA GCAGCAGCGG
    GGACAAGGCA CCATGAACCC TCCACACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe37421
    Clone ID Related Accession (Same CDS sequence) XM_014115129.1
    Accession Version XM_014115129.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3498bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product protein SMG7 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726067.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATATAAGTCC 
    CAGATGAGGA CCGGAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG
    GCAGAAGTCC TGAAGGCTGA TATGACAGAT TCTAAGCTGG GTCCAGCTGA GGTCTGGACA
    TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTAG TTACTGATTT GGAATACGCT
    TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCATGCCT TTAAGAATCA GATCACAACA
    CTACAAGGCC AGGCAAAGAA TCGAGCAAAC CCGAATCGGA GTGAAGTTCA GGCAAACCTT
    TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA
    GTGTTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AATTGGGAAT TATCAGCAAT
    AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GTTCCTGTTC TTATATCTGC
    CAGCACTGCC TCGTCCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA
    GAGTCCTACT ATAGGCATGC AGCTCAGCTT GTCCCCTCTA ATGGTCAGCC TTACAATCAG
    TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACCA CAATTTTCTA CTACTGCAGA
    AGCATCGCTG TGAAGTTCCC TTTCCCAGCT GCCTCCACGA ATCTACAAAA AGCACTTTCT
    AAAGCACTGG AAAGCCGGGA TGAGGTGAAA ACCAAATGGG GTGTTTCTGA CTTCATCAAG
    GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GCTGAGCCCT
    CTTCGAGAGA AATTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAAAAAGC TTTCAACTCT
    CAGCAGTTAG TTCATGTCAC TGTCATTAAC CTGTTTCAAC TTCATCACCT TCGTGACTTT
    AGCAATGAAA CGGAGCAGCA TAGTTATAGC CAAGATGAGC AGCTGTGTTG GACACAGTTA
    CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTCCA GAACAAGTCT
    CAGGAGGAGT CCTACAATGC CTATCCTCTT CCTGCAGTCA AGGTCTCCAT GGACTGGCTG
    AGACTCAGAC CCAGGGTCTT TCAGGAGGCA GTGGTAGATG AAAGACAGTA CATTTGGCCC
    TGGCTAATTT CTCTTCTAAA TAGTTTCCAT CCCCATGAAG AGGATCTTTC AAGTACTAAT
    GCTACACCAC TTCCAGAGGA GTTTGAATTA CAAGGATTCT TGGCTTTGAG ACCTTCTTTC
    AGGAACTTGG ATTTTTCCAA AGGCCATCAG GGTATTACAG GAGACAAAGA AGGTCAGCAA
    CGGCAAATAC GACAGCAGCG CTTGATCTCT ATAGGCAAAT GGATTGCCGA TAATCAGCCA
    AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAG
    TTAACACTAG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCCAGA AACATCCATG
    ATAGAGTCAC TATCTGCAGA CGGGAACCCA GGACTGAAAT CAGTGCTGTC CACGGGCCGT
    AATCTAAGCA ACAACTGTGA CCCCGGAGAG AAACCTATGG TCACCTTCAA AGAGAACATT
    AAGCCGCGAG AAGTGAACAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTGAGAAGG
    GACTATAGCA AAGGAATTAC TATAACTAAG AATGATGGAA AGAAGGACAA CAGCAAGAGG
    AAAACAGAAA CCAAGAAATG CACCTTAGAA AAGTTACAGG AAACAGGAAA GCAGAGTGTG
    GCAGTGCAGG TAAAATCCCA GACAGAATTA AGAAAGACTC CAGTGTCTGA AGCCAGGAAA
    ACCCCTGTAA CTCAAACCCC AAGTCAAGCA ACTAACTCCC AGTTCATCCC CATTCATCAC
    CCTGGAGCCT TCCCTCCTCT TCCCAGCCGG CCAGGGTTCC CGCCCCCTAC ATATGTTATT
    CCCCCTCCTG TGGCATTTTC TATGGGCTCA GGTTACACCT TCCCAGCTGG TGTTTCTGTC
    CCAGGAACCT TTCTTCAGCC TACAGCTCAC TCTCCAGCAG GAAACCAGGT GCAAGCTGGG
    AAACAGTCCC ACATTCCTTA CAGCCAGCAA CGGCCCTCTG GACCAGGGCC AATGAACCAG
    GGACCTCAAC AACCACAGCC ACCTTCCCAG CAATCCCTTA CATCTTTACC AGCTCAGCCA
    ACAGCACAGT CTACAAGCCA GTTGCAGGTT CAAGCTCTAG CTCAGCAACA ATCCCCCACA
    AAAGCTGTGC CAGCTTTGGG GAAAAGCCCT CCTCACCACT CTGGATTCCA GCAGTATCAA
    CAGGCAGATG CCTCCAAACA GCTGTGGAAT CCCCCTCAGG TTCAAGGCCC ATTAGGGAAA
    ATCATGCCTG TGAAACAGCC CTACTACCTT CAGTCCCAAG ACCCCATAAA ACTGTTTGAG
    CCGTCATTGC AACCTCCGGT AATGCAGCAG CAGCCTCTAG AGAAAAAAAT GAAGCCTTTT
    CCCATGGAGC CATATAACCA TAATCCCTCA GAAGTCAAGG TCCCAGAATT CTACTGGGAT
    TCTTCATACA GCATGGCTGA TAACAGAGCT GTAATGGCAC AGCAAGCAAA TATGGACCGC
    AGGGGCAAAC GGTCACCAGG AGTCTTCCGT CCAGAGCAGG ATCCTGTGCC CAGGATGCCA
    TTTGAGAAAT CCTTGTTGGA GAAGCCCTCG GAGCTCATGT CCCATTCCTC CTCTTTCTTG
    TCCCTCACCG GATTCTCTCT CAATCAGGAA AGATACCCAA ATAACAGTGT GTTTAATGAG
    GTATATGGGA AAAACCTGAC AACCAGCTCC AAATCAGAAC TTAATCCTTC GCTGGCCCCC
    CAGGAGACGT CACTCTATTC CCTCTTTGAA GGGACCCCCT GGTCTCCATC ACTTCCTGCC
    AGTTCAGATC ATTCAACACC AGCCAGCCAG TCTCCTCATT CCTCTAACCC AAGCAGCCTG
    CCAAGCTCCC CTCCAACACA CAACCATAAT TCTGTTCCAT TCTCCAACTT TGGACCCATT
    GGGACTCCAG ATAACAGGGA TAGGAGAACC GCAGATCGGT GGAAAACCGA TAAGCCAGCC
    ATGGGTGGGT TTGGCATTGA CTATCTCTCA GCAACATCAT CCTCTGAGAG CAGTTGGCAT
    CAGGCCAGCA CTGCAAGTGG CACCTGGACA GGCCACGGCC CCTCCATGGA GGATTCCTCT
    GCTGTCCTCA TGGAAAGCCT CAAGTCTATC TGGTCCAGTT CCATGATGCA TCCTGGACCT
    TCCGCTCTGG AGCAGCTGTT AATGCAGCAG AAGCAGAAGC AGCAGCGGGG ACAAGGCACC
    ATGAACCCTC CACACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013970604.1
    CDS24..3521
    Translation

    Target ORF information:

    RefSeq Version XM_014115129.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014115129.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATATAAGTCC 
    CAGATGAGGA CCGGAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG
    GCAGAAGTCC TGAAGGCTGA TATGACAGAT TCTAAGCTGG GTCCAGCTGA GGTCTGGACA
    TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTAG TTACTGATTT GGAATACGCT
    TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCATGCCT TTAAGAATCA GATCACAACA
    CTACAAGGCC AGGCAAAGAA TCGAGCAAAC CCGAATCGGA GTGAAGTTCA GGCAAACCTT
    TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA
    GTGTTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AATTGGGAAT TATCAGCAAT
    AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GTTCCTGTTC TTATATCTGC
    CAGCACTGCC TCGTCCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA
    GAGTCCTACT ATAGGCATGC AGCTCAGCTT GTCCCCTCTA ATGGTCAGCC TTACAATCAG
    TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACCA CAATTTTCTA CTACTGCAGA
    AGCATCGCTG TGAAGTTCCC TTTCCCAGCT GCCTCCACGA ATCTACAAAA AGCACTTTCT
    AAAGCACTGG AAAGCCGGGA TGAGGTGAAA ACCAAATGGG GTGTTTCTGA CTTCATCAAG
    GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GCTGAGCCCT
    CTTCGAGAGA AATTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAAAAAGC TTTCAACTCT
    CAGCAGTTAG TTCATGTCAC TGTCATTAAC CTGTTTCAAC TTCATCACCT TCGTGACTTT
    AGCAATGAAA CGGAGCAGCA TAGTTATAGC CAAGATGAGC AGCTGTGTTG GACACAGTTA
    CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTCCA GAACAAGTCT
    CAGGAGGAGT CCTACAATGC CTATCCTCTT CCTGCAGTCA AGGTCTCCAT GGACTGGCTG
    AGACTCAGAC CCAGGGTCTT TCAGGAGGCA GTGGTAGATG AAAGACAGTA CATTTGGCCC
    TGGCTAATTT CTCTTCTAAA TAGTTTCCAT CCCCATGAAG AGGATCTTTC AAGTACTAAT
    GCTACACCAC TTCCAGAGGA GTTTGAATTA CAAGGATTCT TGGCTTTGAG ACCTTCTTTC
    AGGAACTTGG ATTTTTCCAA AGGCCATCAG GGTATTACAG GAGACAAAGA AGGTCAGCAA
    CGGCAAATAC GACAGCAGCG CTTGATCTCT ATAGGCAAAT GGATTGCCGA TAATCAGCCA
    AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAG
    TTAACACTAG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCCAGA AACATCCATG
    ATAGAGTCAC TATCTGCAGA CGGGAACCCA GGACTGAAAT CAGTGCTGTC CACGGGCCGT
    AATCTAAGCA ACAACTGTGA CCCCGGAGAG AAACCTATGG TCACCTTCAA AGAGAACATT
    AAGCCGCGAG AAGTGAACAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTGAGAAGG
    GACTATAGCA AAGGAATTAC TATAACTAAG AATGATGGAA AGAAGGACAA CAGCAAGAGG
    AAAACAGAAA CCAAGAAATG CACCTTAGAA AAGTTACAGG AAACAGGAAA GCAGAGTGTG
    GCAGTGCAGG TAAAATCCCA GACAGAATTA AGAAAGACTC CAGTGTCTGA AGCCAGGAAA
    ACCCCTGTAA CTCAAACCCC AAGTCAAGCA ACTAACTCCC AGTTCATCCC CATTCATCAC
    CCTGGAGCCT TCCCTCCTCT TCCCAGCCGG CCAGGGTTCC CGCCCCCTAC ATATGTTATT
    CCCCCTCCTG TGGCATTTTC TATGGGCTCA GGTTACACCT TCCCAGCTGG TGTTTCTGTC
    CCAGGAACCT TTCTTCAGCC TACAGCTCAC TCTCCAGCAG GAAACCAGGT GCAAGCTGGG
    AAACAGTCCC ACATTCCTTA CAGCCAGCAA CGGCCCTCTG GACCAGGGCC AATGAACCAG
    GGACCTCAAC AACCACAGCC ACCTTCCCAG CAATCCCTTA CATCTTTACC AGCTCAGCCA
    ACAGCACAGT CTACAAGCCA GTTGCAGGTT CAAGCTCTAG CTCAGCAACA ATCCCCCACA
    AAAGCTGTGC CAGCTTTGGG GAAAAGCCCT CCTCACCACT CTGGATTCCA GCAGTATCAA
    CAGGCAGATG CCTCCAAACA GCTGTGGAAT CCCCCTCAGG TTCAAGGCCC ATTAGGGAAA
    ATCATGCCTG TGAAACAGCC CTACTACCTT CAGTCCCAAG ACCCCATAAA ACTGTTTGAG
    CCGTCATTGC AACCTCCGGT AATGCAGCAG CAGCCTCTAG AGAAAAAAAT GAAGCCTTTT
    CCCATGGAGC CATATAACCA TAATCCCTCA GAAGTCAAGG TCCCAGAATT CTACTGGGAT
    TCTTCATACA GCATGGCTGA TAACAGAGCT GTAATGGCAC AGCAAGCAAA TATGGACCGC
    AGGGGCAAAC GGTCACCAGG AGTCTTCCGT CCAGAGCAGG ATCCTGTGCC CAGGATGCCA
    TTTGAGAAAT CCTTGTTGGA GAAGCCCTCG GAGCTCATGT CCCATTCCTC CTCTTTCTTG
    TCCCTCACCG GATTCTCTCT CAATCAGGAA AGATACCCAA ATAACAGTGT GTTTAATGAG
    GTATATGGGA AAAACCTGAC AACCAGCTCC AAATCAGAAC TTAATCCTTC GCTGGCCCCC
    CAGGAGACGT CACTCTATTC CCTCTTTGAA GGGACCCCCT GGTCTCCATC ACTTCCTGCC
    AGTTCAGATC ATTCAACACC AGCCAGCCAG TCTCCTCATT CCTCTAACCC AAGCAGCCTG
    CCAAGCTCCC CTCCAACACA CAACCATAAT TCTGTTCCAT TCTCCAACTT TGGACCCATT
    GGGACTCCAG ATAACAGGGA TAGGAGAACC GCAGATCGGT GGAAAACCGA TAAGCCAGCC
    ATGGGTGGGT TTGGCATTGA CTATCTCTCA GCAACATCAT CCTCTGAGAG CAGTTGGCAT
    CAGGCCAGCA CTGCAAGTGG CACCTGGACA GGCCACGGCC CCTCCATGGA GGATTCCTCT
    GCTGTCCTCA TGGAAAGCCT CAAGTCTATC TGGTCCAGTT CCATGATGCA TCCTGGACCT
    TCCGCTCTGG AGCAGCTGTT AATGCAGCAG AAGCAGAAGC AGCAGCGGGG ACAAGGCACC
    ATGAACCCTC CACACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe37422
    Clone ID Related Accession (Same CDS sequence) XM_014115130.1
    Accession Version XM_014115130.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3360bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product protein SMG7 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726067.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATATAAGTCC 
    CAGATGAGGA CCGGAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG
    GCAGAAGTCC TGAAGGCTGA TATGACAGAT TCTAAGCTGG GTCCAGCTGA GGTCTGGACA
    TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTAG TTACTGATTT GGAATACGCT
    TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCATGCCT TTAAGAATCA GATCACAACA
    CTACAAGGCC AGGCAAAGAA TCGAGCAAAC CCGAATCGGA GTGAAGTTCA GGCAAACCTT
    TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA
    GTGTTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AATTGGGAAT TATCAGCAAT
    AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GTTCCTGTTC TTATATCTGC
    CAGCACTGCC TCGTCCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA
    GAGTCCTACT ATAGGCATGC AGCTCAGCTT GTCCCCTCTA ATGGTCAGCC TTACAATCAG
    TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACCA CAATTTTCTA CTACTGCAGA
    AGCATCGCTG TGAAGTTCCC TTTCCCAGCT GCCTCCACGA ATCTACAAAA AGCACTTTCT
    AAAGCACTGG AAAGCCGGGA TGAGGTGAAA ACCAAATGGG GTGTTTCTGA CTTCATCAAG
    GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GCTGAGCCCT
    CTTCGAGAGA AATTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAAAAAGC TTTCAACTCT
    CAGCAGTTAG TTCATGTCAC TGTCATTAAC CTGTTTCAAC TTCATCACCT TCGTGACTTT
    AGCAATGAAA CGGAGCAGCA TAGTTATAGC CAAGATGAGC AGCTGTGTTG GACACAGTTA
    CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTCCA GAACAAGTCT
    CAGGAGGAGT CCTACAATGC CTATCCTCTT CCTGCAGTCA AGGTCTCCAT GGACTGGCTG
    AGACTCAGAC CCAGGGTCTT TCAGGAGGCA GTGGTAGATG AAAGACAGTA CATTTGGCCC
    TGGCTAATTT CTCTTCTAAA TAGTTTCCAT CCCCATGAAG AGGATCTTTC AAGTACTAAT
    GCTACACCAC TTCCAGAGGA GTTTGAATTA CAAGGATTCT TGGCTTTGAG ACCTTCTTTC
    AGGAACTTGG ATTTTTCCAA AGGCCATCAG GGTATTACAG GAGACAAAGA AGGTCAGCAA
    CGGCAAATAC GACAGCAGCG CTTGATCTCT ATAGGCAAAT GGATTGCCGA TAATCAGCCA
    AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAG
    TTAACACTAG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCCAGA AACATCCATG
    ATAGAGTCAC TATCTGCAGA CGGGAACCCA GGACTGAAAT CAGTGCTGTC CACGGGCCGT
    AATCTAAGCA ACAACTGTGA CCCCGGAGAG AAACCTATGG TCACCTTCAA AGAGAACATT
    AAGCCGCGAG AAGTGAACAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTAAAATCC
    CAGACAGAAT TAAGAAAGAC TCCAGTGTCT GAAGCCAGGA AAACCCCTGT AACTCAAACC
    CCAAGTCAAG CAACTAACTC CCAGTTCATC CCCATTCATC ACCCTGGAGC CTTCCCTCCT
    CTTCCCAGCC GGCCAGGGTT CCCGCCCCCT ACATATGTTA TTCCCCCTCC TGTGGCATTT
    TCTATGGGCT CAGGTTACAC CTTCCCAGCT GGTGTTTCTG TCCCAGGAAC CTTTCTTCAG
    CCTACAGCTC ACTCTCCAGC AGGAAACCAG GTGCAAGCTG GGAAACAGTC CCACATTCCT
    TACAGCCAGC AACGGCCCTC TGGACCAGGG CCAATGAACC AGGGACCTCA ACAACCACAG
    CCACCTTCCC AGCAATCCCT TACATCTTTA CCAGCTCAGC CAACAGCACA GTCTACAAGC
    CAGTTGCAGG TTCAAGCTCT AGCTCAGCAA CAATCCCCCA CAAAAGCTGT GCCAGCTTTG
    GGGAAAAGCC CTCCTCACCA CTCTGGATTC CAGCAGTATC AACAGGCAGA TGCCTCCAAA
    CAGCTGTGGA ATCCCCCTCA GGTTCAAGGC CCATTAGGGA AAATCATGCC TGTGAAACAG
    CCCTACTACC TTCAGTCCCA AGACCCCATA AAACTGTTTG AGCCGTCATT GCAACCTCCG
    GTAATGCAGC AGCAGCCTCT AGAGAAAAAA ATGAAGCCTT TTCCCATGGA GCCATATAAC
    CATAATCCCT CAGAAGTCAA GGTCCCAGAA TTCTACTGGG ATTCTTCATA CAGCATGGCT
    GATAACAGAG CTGTAATGGC ACAGCAAGCA AATATGGACC GCAGGGGCAA ACGGTCACCA
    GGAGTCTTCC GTCCAGAGCA GGATCCTGTG CCCAGGATGC CATTTGAGAA ATCCTTGTTG
    GAGAAGCCCT CGGAGCTCAT GTCCCATTCC TCCTCTTTCT TGTCCCTCAC CGGATTCTCT
    CTCAATCAGG AAAGATACCC AAATAACAGT GTGTTTAATG AGGTATATGG GAAAAACCTG
    ACAACCAGCT CCAAATCAGA ACTTAATCCT TCGCTGGCCC CCCAGGAGAC GTCACTCTAT
    TCCCTCTTTG AAGGGACCCC CTGGTCTCCA TCACTTCCTG CCAGTTCAGA TCATTCAACA
    CCAGCCAGCC AGTCTCCTCA TTCCTCTAAC CCAAGCAGCC TGCCAAGCTC CCCTCCAACA
    CACAACCATA ATTCTGTTCC ATTCTCCAAC TTTGGACCCA TTGGGACTCC AGATAACAGG
    GATAGGAGAA CCGCAGATCG GTGGAAAACC GATAAGCCAG CCATGGGTGG GTTTGGCATT
    GACTATCTCT CAGCAACATC ATCCTCTGAG AGCAGTTGGC ATCAGGCCAG CACTGCAAGT
    GGCACCTGGA CAGGCCACGG CCCCTCCATG GAGGATTCCT CTGCTGTCCT CATGGAAAGC
    CTCAAGTCTA TCTGGTCCAG TTCCATGATG CATCCTGGAC CTTCCGCTCT GGAGCAGCTG
    TTAATGCAGC AGAAGCAGAA GCAGCAGCGG GGACAAGGCA CCATGAACCC TCCACACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013970605.1
    CDS24..3383
    Translation

    Target ORF information:

    RefSeq Version XM_014115130.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014115130.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATATAAGTCC 
    CAGATGAGGA CCGGAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG
    GCAGAAGTCC TGAAGGCTGA TATGACAGAT TCTAAGCTGG GTCCAGCTGA GGTCTGGACA
    TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTAG TTACTGATTT GGAATACGCT
    TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCATGCCT TTAAGAATCA GATCACAACA
    CTACAAGGCC AGGCAAAGAA TCGAGCAAAC CCGAATCGGA GTGAAGTTCA GGCAAACCTT
    TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA
    GTGTTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AATTGGGAAT TATCAGCAAT
    AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GTTCCTGTTC TTATATCTGC
    CAGCACTGCC TCGTCCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA
    GAGTCCTACT ATAGGCATGC AGCTCAGCTT GTCCCCTCTA ATGGTCAGCC TTACAATCAG
    TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACCA CAATTTTCTA CTACTGCAGA
    AGCATCGCTG TGAAGTTCCC TTTCCCAGCT GCCTCCACGA ATCTACAAAA AGCACTTTCT
    AAAGCACTGG AAAGCCGGGA TGAGGTGAAA ACCAAATGGG GTGTTTCTGA CTTCATCAAG
    GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GCTGAGCCCT
    CTTCGAGAGA AATTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAAAAAGC TTTCAACTCT
    CAGCAGTTAG TTCATGTCAC TGTCATTAAC CTGTTTCAAC TTCATCACCT TCGTGACTTT
    AGCAATGAAA CGGAGCAGCA TAGTTATAGC CAAGATGAGC AGCTGTGTTG GACACAGTTA
    CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTCCA GAACAAGTCT
    CAGGAGGAGT CCTACAATGC CTATCCTCTT CCTGCAGTCA AGGTCTCCAT GGACTGGCTG
    AGACTCAGAC CCAGGGTCTT TCAGGAGGCA GTGGTAGATG AAAGACAGTA CATTTGGCCC
    TGGCTAATTT CTCTTCTAAA TAGTTTCCAT CCCCATGAAG AGGATCTTTC AAGTACTAAT
    GCTACACCAC TTCCAGAGGA GTTTGAATTA CAAGGATTCT TGGCTTTGAG ACCTTCTTTC
    AGGAACTTGG ATTTTTCCAA AGGCCATCAG GGTATTACAG GAGACAAAGA AGGTCAGCAA
    CGGCAAATAC GACAGCAGCG CTTGATCTCT ATAGGCAAAT GGATTGCCGA TAATCAGCCA
    AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAG
    TTAACACTAG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCCAGA AACATCCATG
    ATAGAGTCAC TATCTGCAGA CGGGAACCCA GGACTGAAAT CAGTGCTGTC CACGGGCCGT
    AATCTAAGCA ACAACTGTGA CCCCGGAGAG AAACCTATGG TCACCTTCAA AGAGAACATT
    AAGCCGCGAG AAGTGAACAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTAAAATCC
    CAGACAGAAT TAAGAAAGAC TCCAGTGTCT GAAGCCAGGA AAACCCCTGT AACTCAAACC
    CCAAGTCAAG CAACTAACTC CCAGTTCATC CCCATTCATC ACCCTGGAGC CTTCCCTCCT
    CTTCCCAGCC GGCCAGGGTT CCCGCCCCCT ACATATGTTA TTCCCCCTCC TGTGGCATTT
    TCTATGGGCT CAGGTTACAC CTTCCCAGCT GGTGTTTCTG TCCCAGGAAC CTTTCTTCAG
    CCTACAGCTC ACTCTCCAGC AGGAAACCAG GTGCAAGCTG GGAAACAGTC CCACATTCCT
    TACAGCCAGC AACGGCCCTC TGGACCAGGG CCAATGAACC AGGGACCTCA ACAACCACAG
    CCACCTTCCC AGCAATCCCT TACATCTTTA CCAGCTCAGC CAACAGCACA GTCTACAAGC
    CAGTTGCAGG TTCAAGCTCT AGCTCAGCAA CAATCCCCCA CAAAAGCTGT GCCAGCTTTG
    GGGAAAAGCC CTCCTCACCA CTCTGGATTC CAGCAGTATC AACAGGCAGA TGCCTCCAAA
    CAGCTGTGGA ATCCCCCTCA GGTTCAAGGC CCATTAGGGA AAATCATGCC TGTGAAACAG
    CCCTACTACC TTCAGTCCCA AGACCCCATA AAACTGTTTG AGCCGTCATT GCAACCTCCG
    GTAATGCAGC AGCAGCCTCT AGAGAAAAAA ATGAAGCCTT TTCCCATGGA GCCATATAAC
    CATAATCCCT CAGAAGTCAA GGTCCCAGAA TTCTACTGGG ATTCTTCATA CAGCATGGCT
    GATAACAGAG CTGTAATGGC ACAGCAAGCA AATATGGACC GCAGGGGCAA ACGGTCACCA
    GGAGTCTTCC GTCCAGAGCA GGATCCTGTG CCCAGGATGC CATTTGAGAA ATCCTTGTTG
    GAGAAGCCCT CGGAGCTCAT GTCCCATTCC TCCTCTTTCT TGTCCCTCAC CGGATTCTCT
    CTCAATCAGG AAAGATACCC AAATAACAGT GTGTTTAATG AGGTATATGG GAAAAACCTG
    ACAACCAGCT CCAAATCAGA ACTTAATCCT TCGCTGGCCC CCCAGGAGAC GTCACTCTAT
    TCCCTCTTTG AAGGGACCCC CTGGTCTCCA TCACTTCCTG CCAGTTCAGA TCATTCAACA
    CCAGCCAGCC AGTCTCCTCA TTCCTCTAAC CCAAGCAGCC TGCCAAGCTC CCCTCCAACA
    CACAACCATA ATTCTGTTCC ATTCTCCAAC TTTGGACCCA TTGGGACTCC AGATAACAGG
    GATAGGAGAA CCGCAGATCG GTGGAAAACC GATAAGCCAG CCATGGGTGG GTTTGGCATT
    GACTATCTCT CAGCAACATC ATCCTCTGAG AGCAGTTGGC ATCAGGCCAG CACTGCAAGT
    GGCACCTGGA CAGGCCACGG CCCCTCCATG GAGGATTCCT CTGCTGTCCT CATGGAAAGC
    CTCAAGTCTA TCTGGTCCAG TTCCATGATG CATCCTGGAC CTTCCGCTCT GGAGCAGCTG
    TTAATGCAGC AGAAGCAGAA GCAGCAGCGG GGACAAGGCA CCATGAACCC TCCACACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.