Smg7 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Smg7
Entrez Gene ID	360855
Full Name	SMG7 nonsense mediated mRNA decay factor
Synonyms	RGD1310761
General protein information	Preferred Names SMG7 nonsense mediated mRNA decay factor Names protein SMG7 Smg-7 homolog, nonsense mediated mRNA decay factor
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 13 Map Location: 13q21

mRNA and Protein(s)

mRNA	Protein	Name
XM_006250006.3	XP_006250068.1	protein SMG7 isoform X2
XM_008769655.2	XP_008767877.1	protein SMG7 isoform X3
XM_006250005.3	XP_006250067.1	protein SMG7 isoform X1
XM_008769656.2	XP_008767878.1	protein SMG7 isoform X4
NM_001191549.1	NP_001178478.1	protein SMG7

Pathways from BioSystems

Homologies

	SMG7	XP_002802068.1
Mus musculus (house mouse)	Smg7	NP_001005507.1
Danio rerio (zebrafish)	smg7	NP_001038548.2
Bos taurus (cattle)	SMG7	NP_001180134.2
Xenopus tropicalis (tropical clawed frog)	smg7	NP_989258.1
Homo sapiens (human)	SMG7	NP_775179.1
Pan troglodytes (chimpanzee)	SMG7	XP_514048.3
Gallus gallus (chicken)	SMG7	XP_004943329.1
Canis lupus familiaris (dog)	SMG7	XP_537157.4
Rattus norvegicus (Norway rat)	Smg7	NP_001178478.1

Gene Ontology
Bibliography

Related articles in PubMed

Telomeric repeat containing RNA and RNA surveillance factors at mammalian chromosome ends.
Azzalin CM, Reichenbach P, Khoriauli L, Giulotto E, Lingner J
Science (New York, N.Y.)318(5851)798-801(2007 Nov)

Phosphorylation of hUPF1 induces formation of mRNA surveillance complexes containing hSMG-5 and hSMG-7.
Ohnishi T, Yamashita A, Kashima I, Schell T, Anders KR, Grimson A, Hachiya T, Hentze MW, Anderson P, Ohno S
Molecular cell12(5)1187-200(2003 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Smg7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Smg7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa06959	NM_001191549.1 Latest version!	Rattus norvegicus SMG7 nonsense mediated mRNA decay factor (Smg7), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
ORa35452	XM_006250005.3 Latest version!	Rattus norvegicus SMG7 nonsense mediated mRNA decay factor (Smg7), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
ORa35453	XM_006250006.3 Latest version!	Rattus norvegicus SMG7 nonsense mediated mRNA decay factor (Smg7), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
ORa35455	XM_008769655.2 Latest version!	Rattus norvegicus SMG7 nonsense mediated mRNA decay factor (Smg7), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
ORa35456	XM_008769656.2 Latest version!	Rattus norvegicus SMG7 nonsense mediated mRNA decay factor (Smg7), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Smg7 ( NM_001191549.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001178478.1 Rattus norvegicus SMG7 nonsense mediated mRNA decay factor (Smg7), mRNA.

Price & Availability↑

CloneID	ORa06959
Clone ID Related Accession (Same CDS sequence)	NM_001191549.1
Accession Version	NM_001191549.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3450bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-31
Organism	Rattus norvegicus(Norway rat)
Product	protein SMG7
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AABR07021404.1, AABR07021403.1 and AABR07021402.1. On Jul 17, 2010 this sequence version replaced XM_002728013.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGAGGACCG AAAACTTGAA ATCAGAGGAG CATCTGAAAT CAAGTAATAT TAGGCAGGCA 
GAAGTCCTGA AGGCTGAAAT GACAGATTCT AAGCTGGGTC CAGCTGAGGT CTGGACATCC 
AGGCAAGCTC TGCAGGACTT GTACCAGAAA ATGCTGGTTA CTGATCTGGA GTATGCTTTA 
GACAAGAAGG TAGAGCAGGA CCTCTGGAAT CATGCCTTTA AGAATCAGAT CACAACACTA 
CAAGGCCAAG CCAAGAATCG AGCAAACCCA AATCGGAGTG AAGTCCAGGC AAACCTTTCT 
CTGTTCCTAG AGGCAGCTAG CGGCTTCTAT ACTCAGTTAT TACAGGAACT GTGTACGGTG 
TTTAATGTAG ATTTGCCATG CCGTGTGAAG TCTTCCCAGT TGGGAATTAT TAGCAATAAA 
CAGACGCACA CCAGCGCCAT AGTGAAGCCA CAGTCTAGCT CCTGTTCCTA CATTTGCCAG 
CACTGCCTCG TCCACCTTGG AGACATTGCT CGATACAGAA ACCAGACTAG CCAGGCAGAG 
TCCTACTATA GGCATGCAGC TCAGCTTGTC CCCTCCAATG GTCAGCCTTA CAATCAGTTG 
GCTATCTTAG CTTCTTCCAA AGGAGACCAC CTGACCACAA TTTTCTACTA CTGCAGAAGC 
ATTGCTGTGA AATTCCCTTT CCCAGCTGCC TCCACTAACC TACAAAAAGC ACTTTCTAAA 
GCACTGGAAA GCCGGGATGA GTTGAAAACC AAGTGGGGTG TTTCTGACTT CATCAAGGCC 
TTTATTAAAT TCCACGGTCA TGTATACCTG AGTAAGAGTT TGGAAAAGCT GAGCCCTCTT 
CGAGAGAAGT TAGAAGAACA GTTTAAGAGA CTGCTGTTCC AAAAAGCTTT CAACTCTCAG 
CAGTTAGTTC ATGTCACTGT CATTAACCTG TTTCAACTTC ATCATCTTCG TGACTTTAGC 
AATGAAACAG AGCAGCACAG TTACAGCCAA GATGAACAGC TGTGTTGGAC ACAGTTGCTG 
GCCCTCTTCA TGTCTTTTCT TGGCATCCTG TGCAAATGTC CTCTCCAGAA TGATTCTCAG 
GAGTCCTACA ATGCCTATCC CCTTCCTGCA GTCAAGGTCT CCATGGACTG GCTAAGACTC 
CGACCTAGAG TCTTTCAAGA AGCTGTAGTG GATGAAAGAC AGTACATCTG GCCCTGGCTA 
ATTTCTCTTC TCAATAGTTT TCATCCCCGT GAAGATGATC TCTCAAGTAC TAATGCCACA 
CCACTTCCAG AGGAGTTTGA ACTACAAGGG TTCCTGGCTT TGAGACCTTC TTTCAGGAAC 
TTGGATTTTT CCAAAGGCCA TCAGGGTATT ACAGGAGACA AAGAGGGTCA ACAACGGCGA 
ATACGGCAGC AGCGTTTGAT CTCCATAGGG AAGTGGATTG CTGACAACCA GCCACGGCTG 
ATTCAGTGTG AAAATGAGGT AGGGAAATTG TTATTTATCA CAGAAATCCC AGAATTAATA 
CTAGAAGACC CCAGTGAAGC CAAAGAGAAC CTCATCCTAC AAGAAACATC TGCGATAGAG 
TCACACGCTT CGGACGGGAG CCCAGGACTG AAGTCAGTGC TGTCTACAGG CCGAAGTCCA 
AGCACCAGCT GTGACTCAGG AGAGAAGCCA GTGGTCACCT TCAAAGAGAA CATTAAGCCA 
CGAGAAGTGA ACAGAGACCA AGGAAGAAGC TTTCCTCCCA AAGAGGTAAA ATCCCAGACA 
GAACTAAGAA AGACCCCAGT GTCCGAAGCC AGGAAAACTC CTGTCACTCA AACTCCAAGT 
CAAACAAATA ATTCTCAGTT CATCCCCATC CATCACCCTG GAGCCTTCCC TCCTCTTCCC 
AGCCGACCGG GGTTCCCGCC CCCAACATAT GTTATCCCCC CTCCTGTGGC ATTTTCTATG 
GGCTCAGGAT ACACCTTCCC AGCTGGTGTT TCTGTCCCAG GAACCTTTCT TCAGTCTACA 
GCTCACTCTC CAGCAGGAAA CCAGGTGCAA GCTGGGAAAC AGTCCCACAT TCCTTACAGC 
CAGCAACGGC CCTCCGGACC AGGGCCAATG AACCAGGGAC CTCAACAATC ACAGCCACCT 
TCCCAGCCAC CCCTTACATC TTTACCAGCT CAGCCAACAG CACAGTCTAC AAGCCAGTTG 
CAGGTTCAAG CTCTAGCTCA GCAACAGCAG TCCCCTACAA AAGTCATACC AGCCTTGGGG 
AAAAGCCCGC CTCACCACTC TGGATTCCAG CAGTATCAAC AGGCAGATGC CTCCAAACAG 
CTGTGGAATC CCCCTCAGGT TCAAAGCCCA CTAGGGAAAA TTATGCCTGT GAAACAGCCC 
TACTACCTTC AGACCCAAGA CCCTATAAAA CTGTTTGAGC CGTCATTGCA GCCTCCTGTA 
ATACAGCAAC AGCCTCTAGA GAAAAAAATG AAGCCTTTCC CCATGGAGCC ATATAACCAT 
AATCCCTCAG AAGTCAAGGT CCCAGAGTTC TACTGGGATT CTTCCTACAG CATGGCTGAT 
AACAGAGCAG TAATGGCTCA GCAACCAAAT ATGGACCGCA GGAGCAAACG GTCACCCGGA 
GTCTTCCGTC CAGAGCAGGA TCCTGTGCCC AGGATGCCGT TTGAGGACCC CAAGAGCTCC 
CCTCTGCTTC CTCCGGACCT GTTAAAGAGT CTGGCTGCCT TGGAGGAAGA GGAAGAGCTG 
ATCTTTTCTA ACCCTCCTGA TCTTTACCCA GCTCTGCTGG GTCCTCTCGC CTCTCTTCCT 
GGACGAAGCC TCTTTAAATC CTTACTGGAG AAGCCTTCCG AGCTCATGTC ACATTCATCT 
TCTTTCCTGT CCCTCACTGG GTTCTCTGTC AATCAGGAAA GATATCCAAA CAGCAGTGTG 
TTCAATGAAG TGTATGGGAA GAACCTGACA GCCAGCTCCA AGGCAGAGCT GAGCCCCTCG 
GTTGCCTCCT CCCAGGAAAC ATCACTGTAC TCCCTCTTCG AAGGGACCCC TTGGTCTCCA 
TCACTTCCTG CCAGTTCAGA TCATTCAACA CCAGCCAGCC AGTCTCCTCA TTCCTCCAAC 
CCAAGCAGCC TGCCCAGTTC TCCTCCAACA CATAACCATA ATTCTGCTCC ATTCTCTAAT 
TTTGGACCCA TTGGGACTCC AGATAACAGG GATAGGCGGC CTGCAGATAG GTGGAAAACT 
GATAAACCAG CCATGGGTGG TTTCGGCGTT GATTACCTCT CAGCAACGTC ATCATCTGAG 
AACAGTTGGC ATCAGGCTAG CACTCCAAGT GGCACCTGGA CAGGCCACGG CCCCTCCATG 
GAGGATTCCT CCGCTGTCCT CATGGAAAGC CTAAAGTCTA TCTGGTCCAG TTCCATGATG 
CATCCTGGAC CCTCCGCTCT CGAGCAGTTG TTAATGCAGC AGAAGCAGAA ACAGCAGCGG 
GGACAAGGTG CCATGAACCC TCCACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001178478.1
CDS	1..3450
Translation	MRTENLKSEEHLKSSNIRQAEVLKAEMTDSKLGPAEVWTSRQALQDLYQKMLVTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRANPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQTHTSAIVKPQSSSCSYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNLQKALSKALESRDELKTKWGVSDFIKAFIKFHGHVYLSKSLEKLSPLREKLEEQFKRLLFQKAFNSQQLVHVTVINLFQLHHLRDFSNETEQHSYSQDEQLCWTQLLALFMSFLGILCKCPLQNDSQESYNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPREDDLSSTNATPLPEEFELQGFLALRPSFRNLDFSKGHQGITGDKEGQQRRIRQQRLISIGKWIADNQPRLIQCENEVGKLLFITEIPELILEDPSEAKENLILQETSAIESHASDGSPGLKSVLSTGRSPSTSCDSGEKPVVTFKENIKPREVNRDQGRSFPPKEVKSQTELRKTPVSEARKTPVTQTPSQTNNSQFIPIHHPGAFPPLPSRPGFPPPTYVIPPPVAFSMGSGYTFPAGVSVPGTFLQSTAHSPAGNQVQAGKQSHIPYSQQRPSGPGPMNQGPQQSQPPSQPPLTSLPAQPTAQSTSQLQVQALAQQQQSPTKVIPALGKSPPHHSGFQQYQQADASKQLWNPPQVQSPLGKIMPVKQPYYLQTQDPIKLFEPSLQPPVIQQQPLEKKMKPFPMEPYNHNPSEVKVPEFYWDSSYSMADNRAVMAQQPNMDRRSKRSPGVFRPEQDPVPRMPFEDPKSSPLLPPDLLKSLAALEEEEELIFSNPPDLYPALLGPLASLPGRSLFKSLLEKPSELMSHSSSFLSLTGFSVNQERYPNSSVFNEVYGKNLTASSKAELSPSVASSQETSLYSLFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSAPFSNFGPIGTPDNRDRRPADRWKTDKPAMGGFGVDYLSATSSSENSWHQASTPSGTWTGHGPSMEDSSAVLMESLKSIWSSSMMHPGPSALEQLLMQQKQKQQRGQGAMNPPH

Target ORF information:

RefSeq Version	NM_001191549.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus SMG7 nonsense mediated mRNA decay factor (Smg7), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001191549.1

ORF Insert Sequence:

ATGAGGACCG AAAACTTGAA ATCAGAGGAG CATCTGAAAT CAAGTAATAT TAGGCAGGCA 
GAAGTCCTGA AGGCTGAAAT GACAGATTCT AAGCTGGGTC CAGCTGAGGT CTGGACATCC 
AGGCAAGCTC TGCAGGACTT GTACCAGAAA ATGCTGGTTA CTGATCTGGA GTATGCTTTA 
GACAAGAAGG TAGAGCAGGA CCTCTGGAAT CATGCCTTTA AGAATCAGAT CACAACACTA 
CAAGGCCAAG CCAAGAATCG AGCAAACCCA AATCGGAGTG AAGTCCAGGC AAACCTTTCT 
CTGTTCCTAG AGGCAGCTAG CGGCTTCTAT ACTCAGTTAT TACAGGAACT GTGTACGGTG 
TTTAATGTAG ATTTGCCATG CCGTGTGAAG TCTTCCCAGT TGGGAATTAT TAGCAATAAA 
CAGACGCACA CCAGCGCCAT AGTGAAGCCA CAGTCTAGCT CCTGTTCCTA CATTTGCCAG 
CACTGCCTCG TCCACCTTGG AGACATTGCT CGATACAGAA ACCAGACTAG CCAGGCAGAG 
TCCTACTATA GGCATGCAGC TCAGCTTGTC CCCTCCAATG GTCAGCCTTA CAATCAGTTG 
GCTATCTTAG CTTCTTCCAA AGGAGACCAC CTGACCACAA TTTTCTACTA CTGCAGAAGC 
ATTGCTGTGA AATTCCCTTT CCCAGCTGCC TCCACTAACC TACAAAAAGC ACTTTCTAAA 
GCACTGGAAA GCCGGGATGA GTTGAAAACC AAGTGGGGTG TTTCTGACTT CATCAAGGCC 
TTTATTAAAT TCCACGGTCA TGTATACCTG AGTAAGAGTT TGGAAAAGCT GAGCCCTCTT 
CGAGAGAAGT TAGAAGAACA GTTTAAGAGA CTGCTGTTCC AAAAAGCTTT CAACTCTCAG 
CAGTTAGTTC ATGTCACTGT CATTAACCTG TTTCAACTTC ATCATCTTCG TGACTTTAGC 
AATGAAACAG AGCAGCACAG TTACAGCCAA GATGAACAGC TGTGTTGGAC ACAGTTGCTG 
GCCCTCTTCA TGTCTTTTCT TGGCATCCTG TGCAAATGTC CTCTCCAGAA TGATTCTCAG 
GAGTCCTACA ATGCCTATCC CCTTCCTGCA GTCAAGGTCT CCATGGACTG GCTAAGACTC 
CGACCTAGAG TCTTTCAAGA AGCTGTAGTG GATGAAAGAC AGTACATCTG GCCCTGGCTA 
ATTTCTCTTC TCAATAGTTT TCATCCCCGT GAAGATGATC TCTCAAGTAC TAATGCCACA 
CCACTTCCAG AGGAGTTTGA ACTACAAGGG TTCCTGGCTT TGAGACCTTC TTTCAGGAAC 
TTGGATTTTT CCAAAGGCCA TCAGGGTATT ACAGGAGACA AAGAGGGTCA ACAACGGCGA 
ATACGGCAGC AGCGTTTGAT CTCCATAGGG AAGTGGATTG CTGACAACCA GCCACGGCTG 
ATTCAGTGTG AAAATGAGGT AGGGAAATTG TTATTTATCA CAGAAATCCC AGAATTAATA 
CTAGAAGACC CCAGTGAAGC CAAAGAGAAC CTCATCCTAC AAGAAACATC TGCGATAGAG 
TCACACGCTT CGGACGGGAG CCCAGGACTG AAGTCAGTGC TGTCTACAGG CCGAAGTCCA 
AGCACCAGCT GTGACTCAGG AGAGAAGCCA GTGGTCACCT TCAAAGAGAA CATTAAGCCA 
CGAGAAGTGA ACAGAGACCA AGGAAGAAGC TTTCCTCCCA AAGAGGTAAA ATCCCAGACA 
GAACTAAGAA AGACCCCAGT GTCCGAAGCC AGGAAAACTC CTGTCACTCA AACTCCAAGT 
CAAACAAATA ATTCTCAGTT CATCCCCATC CATCACCCTG GAGCCTTCCC TCCTCTTCCC 
AGCCGACCGG GGTTCCCGCC CCCAACATAT GTTATCCCCC CTCCTGTGGC ATTTTCTATG 
GGCTCAGGAT ACACCTTCCC AGCTGGTGTT TCTGTCCCAG GAACCTTTCT TCAGTCTACA 
GCTCACTCTC CAGCAGGAAA CCAGGTGCAA GCTGGGAAAC AGTCCCACAT TCCTTACAGC 
CAGCAACGGC CCTCCGGACC AGGGCCAATG AACCAGGGAC CTCAACAATC ACAGCCACCT 
TCCCAGCCAC CCCTTACATC TTTACCAGCT CAGCCAACAG CACAGTCTAC AAGCCAGTTG 
CAGGTTCAAG CTCTAGCTCA GCAACAGCAG TCCCCTACAA AAGTCATACC AGCCTTGGGG 
AAAAGCCCGC CTCACCACTC TGGATTCCAG CAGTATCAAC AGGCAGATGC CTCCAAACAG 
CTGTGGAATC CCCCTCAGGT TCAAAGCCCA CTAGGGAAAA TTATGCCTGT GAAACAGCCC 
TACTACCTTC AGACCCAAGA CCCTATAAAA CTGTTTGAGC CGTCATTGCA GCCTCCTGTA 
ATACAGCAAC AGCCTCTAGA GAAAAAAATG AAGCCTTTCC CCATGGAGCC ATATAACCAT 
AATCCCTCAG AAGTCAAGGT CCCAGAGTTC TACTGGGATT CTTCCTACAG CATGGCTGAT 
AACAGAGCAG TAATGGCTCA GCAACCAAAT ATGGACCGCA GGAGCAAACG GTCACCCGGA 
GTCTTCCGTC CAGAGCAGGA TCCTGTGCCC AGGATGCCGT TTGAGGACCC CAAGAGCTCC 
CCTCTGCTTC CTCCGGACCT GTTAAAGAGT CTGGCTGCCT TGGAGGAAGA GGAAGAGCTG 
ATCTTTTCTA ACCCTCCTGA TCTTTACCCA GCTCTGCTGG GTCCTCTCGC CTCTCTTCCT 
GGACGAAGCC TCTTTAAATC CTTACTGGAG AAGCCTTCCG AGCTCATGTC ACATTCATCT 
TCTTTCCTGT CCCTCACTGG GTTCTCTGTC AATCAGGAAA GATATCCAAA CAGCAGTGTG 
TTCAATGAAG TGTATGGGAA GAACCTGACA GCCAGCTCCA AGGCAGAGCT GAGCCCCTCG 
GTTGCCTCCT CCCAGGAAAC ATCACTGTAC TCCCTCTTCG AAGGGACCCC TTGGTCTCCA 
TCACTTCCTG CCAGTTCAGA TCATTCAACA CCAGCCAGCC AGTCTCCTCA TTCCTCCAAC 
CCAAGCAGCC TGCCCAGTTC TCCTCCAACA CATAACCATA ATTCTGCTCC ATTCTCTAAT 
TTTGGACCCA TTGGGACTCC AGATAACAGG GATAGGCGGC CTGCAGATAG GTGGAAAACT 
GATAAACCAG CCATGGGTGG TTTCGGCGTT GATTACCTCT CAGCAACGTC ATCATCTGAG 
AACAGTTGGC ATCAGGCTAG CACTCCAAGT GGCACCTGGA CAGGCCACGG CCCCTCCATG 
GAGGATTCCT CCGCTGTCCT CATGGAAAGC CTAAAGTCTA TCTGGTCCAG TTCCATGATG 
CATCCTGGAC CCTCCGCTCT CGAGCAGTTG TTAATGCAGC AGAAGCAGAA ACAGCAGCGG 
GGACAAGGTG CCATGAACCC TCCACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Smg7 ( XM_006250005.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006250067.1 Rattus norvegicus SMG7 nonsense mediated mRNA decay factor (Smg7), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa35452
Clone ID Related Accession (Same CDS sequence)	XM_006250005.3
Accession Version	XM_006250005.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3639bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	protein SMG7 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006250005.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTTAC AGATAGTCAT TGTGGGAGAA AACGTTTCAT TTAAGTCCCA GATGAGGACC 
GAAAACTTGA AATCAGAGGA GCATCTGAAA TCAAGTAATA TTAGGCAGGC AGAAGTCCTG 
AAGGCTGAAA TGACAGATTC TAAGCTGGGT CCAGCTGAGG TCTGGACATC CAGGCAAGCT 
CTGCAGGACT TGTACCAGAA AATGCTGGTT ACTGATCTGG AGTATGCTTT AGACAAGAAG 
GTAGAGCAGG ACCTCTGGAA TCATGCCTTT AAGAATCAGA TCACAACACT ACAAGGCCAA 
GCCAAGAATC GAGCAAACCC AAATCGGAGT GAAGTCCAGG CAAACCTTTC TCTGTTCCTA 
GAGGCAGCTA GCGGCTTCTA TACTCAGTTA TTACAGGAAC TGTGTACGGT GTTTAATGTA 
GATTTGCCAT GCCGTGTGAA GTCTTCCCAG TTGGGAATTA TTAGCAATAA ACAGACGCAC 
ACCAGCGCCA TAGTGAAGCC ACAGTCTAGC TCCTGTTCCT ACATTTGCCA GCACTGCCTC 
GTCCACCTTG GAGACATTGC TCGATACAGA AACCAGACTA GCCAGGCAGA GTCCTACTAT 
AGGCATGCAG CTCAGCTTGT CCCCTCCAAT GGTCAGCCTT ACAATCAGTT GGCTATCTTA 
GCTTCTTCCA AAGGAGACCA CCTGACCACA ATTTTCTACT ACTGCAGAAG CATTGCTGTG 
AAATTCCCTT TCCCAGCTGC CTCCACTAAC CTACAAAAAG CACTTTCTAA AGCACTGGAA 
AGCCGGGATG AGTTGAAAAC CAAGTGGGGT GTTTCTGACT TCATCAAGGC CTTTATTAAA 
TTCCACGGTC ATGTATACCT GAGTAAGAGT TTGGAAAAGC TGAGCCCTCT TCGAGAGAAG 
TTAGAAGAAC AGTTTAAGAG ACTGCTGTTC CAAAAAGCTT TCAACTCTCA GCAGTTAGTT 
CATGTCACTG TCATTAACCT GTTTCAACTT CATCATCTTC GTGACTTTAG CAATGAAACA 
GAGCAGCACA GTTACAGCCA AGATGAACAG CTGTGTTGGA CACAGTTGCT GGCCCTCTTC 
ATGTCTTTTC TTGGCATCCT GTGCAAATGT CCTCTCCAGA ATGATTCTCA GGAGTCCTAC 
AATGCCTATC CCCTTCCTGC AGTCAAGGTC TCCATGGACT GGCTAAGACT CCGACCTAGA 
GTCTTTCAAG AAGCTGTAGT GGATGAAAGA CAGTACATCT GGCCCTGGCT AATTTCTCTT 
CTCAATAGTT TTCATCCCCG TGAAGATGAT CTCTCAAGTA CTAATGCCAC ACCACTTCCA 
GAGGAGTTTG AACTACAAGG GTTCCTGGCT TTGAGACCTT CTTTCAGGAA CTTGGATTTT 
TCCAAAGGCC ATCAGGGTAT TACAGGAGAC AAAGAGGGTC AACAACGGCG AATACGGCAG 
CAGCGTTTGA TCTCCATAGG GAAGTGGATT GCTGACAACC AGCCACGGCT GATTCAGTGT 
GAAAATGAGG TAGGGAAATT GTTATTTATC ACAGAAATCC CAGAATTAAT ACTAGAAGAC 
CCCAGTGAAG CCAAAGAGAA CCTCATCCTA CAAGAAACAT CTGCGATAGA GTCACACGCT 
TCGGACGGGA GCCCAGGACT GAAGTCAGTG CTGTCTACAG GCCGAAGTCC AAGCACCAGC 
TGTGACTCAG GAGAGAAGCC AGTGGTCACC TTCAAAGAGA ACATTAAGCC ACGAGAAGTG 
AACAGAGACC AAGGAAGAAG CTTTCCTCCC AAAGAGGTGA GAAGGGACTG TAGCAAAGGA 
GTAACTGTAA CTCAGGATGA CGGACAGAAG GACAGCAGCA AGAGGAGAGC TGAGACCAAG 
AGATGCACCT TAGGAAAGTT GCAGGAAACA GGAAAGCAGA GTGTGGCAGT GCAGGTAAAA 
TCCCAGACAG AACTAAGAAA GACCCCAGTG TCCGAAGCCA GGAAAACTCC TGTCACTCAA 
ACTCCAAGTC AAACAAATAA TTCTCAGTTC ATCCCCATCC ATCACCCTGG AGCCTTCCCT 
CCTCTTCCCA GCCGACCGGG GTTCCCGCCC CCAACATATG TTATCCCCCC TCCTGTGGCA 
TTTTCTATGG GCTCAGGATA CACCTTCCCA GCTGGTGTTT CTGTCCCAGG AACCTTTCTT 
CAGTCTACAG CTCACTCTCC AGCAGGAAAC CAGGTGCAAG CTGGGAAACA GTCCCACATT 
CCTTACAGCC AGCAACGGCC CTCCGGACCA GGGCCAATGA ACCAGGGACC TCAACAATCA 
CAGCCACCTT CCCAGCCACC CCTTACATCT TTACCAGCTC AGCCAACAGC ACAGTCTACA 
AGCCAGTTGC AGGTTCAAGC TCTAGCTCAG CAACAGCAGT CCCCTACAAA AGTCATACCA 
GCCTTGGGGA AAAGCCCGCC TCACCACTCT GGATTCCAGC AGTATCAACA GGCAGATGCC 
TCCAAACAGC TGTGGAATCC CCCTCAGGTT CAAAGCCCAC TAGGGAAAAT TATGCCTGTG 
AAACAGCCCT ACTACCTTCA GACCCAAGAC CCTATAAAAC TGTTTGAGCC GTCATTGCAG 
CCTCCTGTAA TACAGCAACA GCCTCTAGAG AAAAAAATGA AGCCTTTCCC CATGGAGCCA 
TATAACCATA ATCCCTCAGA AGTCAAGGTC CCAGAGTTCT ACTGGGATTC TTCCTACAGC 
ATGGCTGATA ACAGAGCAGT AATGGCTCAG CAACCAAATA TGGACCGCAG GAGCAAACGG 
TCACCCGGAG TCTTCCGTCC AGAGCAGGAT CCTGTGCCCA GGATGCCGTT TGAGGACCCC 
AAGAGCTCCC CTCTGCTTCC TCCGGACCTG TTAAAGAGTC TGGCTGCCTT GGAGGAAGAG 
GAAGAGCTGA TCTTTTCTAA CCCTCCTGAT CTTTACCCAG CTCTGCTGGG TCCTCTCGCC 
TCTCTTCCTG GACGAAGCCT CTTTAAATCC TTACTGGAGA AGCCTTCCGA GCTCATGTCA 
CATTCATCTT CTTTCCTGTC CCTCACTGGG TTCTCTGTCA ATCAGGAAAG ATATCCAAAC 
AGCAGTGTGT TCAATGAAGT GTATGGGAAG AACCTGACAG CCAGCTCCAA GGCAGAGCTG 
AGCCCCTCGG TTGCCTCCTC CCAGGAAACA TCACTGTACT CCCTCTTCGA AGGGACCCCT 
TGGTCTCCAT CACTTCCTGC CAGTTCAGAT CATTCAACAC CAGCCAGCCA GTCTCCTCAT 
TCCTCCAACC CAAGCAGCCT GCCCAGTTCT CCTCCAACAC ATAACCATAA TTCTGCTCCA 
TTCTCTAATT TTGGACCCAT TGGGACTCCA GATAACAGGG ATAGGCGGCC TGCAGATAGG 
TGGAAAACTG ATAAACCAGC CATGGGTGGT TTCGGCGTTG ATTACCTCTC AGCAACGTCA 
TCATCTGAGA ACAGTTGGCA TCAGGCTAGC ACTCCAAGTG GCACCTGGAC AGGCCACGGC 
CCCTCCATGG AGGATTCCTC CGCTGTCCTC ATGGAAAGCC TAAAGTCTAT CTGGTCCAGT 
TCCATGATGC ATCCTGGACC CTCCGCTCTC GAGCAGTTGT TAATGCAGCA GAAGCAGAAA 
CAGCAGCGGG GACAAGGTGC CATGAACCCT CCACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006250067.1
CDS	314..3952
Translation	MLLQIVIVGENVSFKSQMRTENLKSEEHLKSSNIRQAEVLKAEMTDSKLGPAEVWTSRQALQDLYQKMLVTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRANPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQTHTSAIVKPQSSSCSYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNLQKALSKALESRDELKTKWGVSDFIKAFIKFHGHVYLSKSLEKLSPLREKLEEQFKRLLFQKAFNSQQLVHVTVINLFQLHHLRDFSNETEQHSYSQDEQLCWTQLLALFMSFLGILCKCPLQNDSQESYNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPREDDLSSTNATPLPEEFELQGFLALRPSFRNLDFSKGHQGITGDKEGQQRRIRQQRLISIGKWIADNQPRLIQCENEVGKLLFITEIPELILEDPSEAKENLILQETSAIESHASDGSPGLKSVLSTGRSPSTSCDSGEKPVVTFKENIKPREVNRDQGRSFPPKEVRRDCSKGVTVTQDDGQKDSSKRRAETKRCTLGKLQETGKQSVAVQVKSQTELRKTPVSEARKTPVTQTPSQTNNSQFIPIHHPGAFPPLPSRPGFPPPTYVIPPPVAFSMGSGYTFPAGVSVPGTFLQSTAHSPAGNQVQAGKQSHIPYSQQRPSGPGPMNQGPQQSQPPSQPPLTSLPAQPTAQSTSQLQVQALAQQQQSPTKVIPALGKSPPHHSGFQQYQQADASKQLWNPPQVQSPLGKIMPVKQPYYLQTQDPIKLFEPSLQPPVIQQQPLEKKMKPFPMEPYNHNPSEVKVPEFYWDSSYSMADNRAVMAQQPNMDRRSKRSPGVFRPEQDPVPRMPFEDPKSSPLLPPDLLKSLAALEEEEELIFSNPPDLYPALLGPLASLPGRSLFKSLLEKPSELMSHSSSFLSLTGFSVNQERYPNSSVFNEVYGKNLTASSKAELSPSVASSQETSLYSLFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSAPFSNFGPIGTPDNRDRRPADRWKTDKPAMGGFGVDYLSATSSSENSWHQASTPSGTWTGHGPSMEDSSAVLMESLKSIWSSSMMHPGPSALEQLLMQQKQKQQRGQGAMNPPH

Target ORF information:

RefSeq Version	XM_006250005.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus SMG7 nonsense mediated mRNA decay factor (Smg7), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006250005.3

ORF Insert Sequence:

ATGCTCTTAC AGATAGTCAT TGTGGGAGAA AACGTTTCAT TTAAGTCCCA GATGAGGACC 
GAAAACTTGA AATCAGAGGA GCATCTGAAA TCAAGTAATA TTAGGCAGGC AGAAGTCCTG 
AAGGCTGAAA TGACAGATTC TAAGCTGGGT CCAGCTGAGG TCTGGACATC CAGGCAAGCT 
CTGCAGGACT TGTACCAGAA AATGCTGGTT ACTGATCTGG AGTATGCTTT AGACAAGAAG 
GTAGAGCAGG ACCTCTGGAA TCATGCCTTT AAGAATCAGA TCACAACACT ACAAGGCCAA 
GCCAAGAATC GAGCAAACCC AAATCGGAGT GAAGTCCAGG CAAACCTTTC TCTGTTCCTA 
GAGGCAGCTA GCGGCTTCTA TACTCAGTTA TTACAGGAAC TGTGTACGGT GTTTAATGTA 
GATTTGCCAT GCCGTGTGAA GTCTTCCCAG TTGGGAATTA TTAGCAATAA ACAGACGCAC 
ACCAGCGCCA TAGTGAAGCC ACAGTCTAGC TCCTGTTCCT ACATTTGCCA GCACTGCCTC 
GTCCACCTTG GAGACATTGC TCGATACAGA AACCAGACTA GCCAGGCAGA GTCCTACTAT 
AGGCATGCAG CTCAGCTTGT CCCCTCCAAT GGTCAGCCTT ACAATCAGTT GGCTATCTTA 
GCTTCTTCCA AAGGAGACCA CCTGACCACA ATTTTCTACT ACTGCAGAAG CATTGCTGTG 
AAATTCCCTT TCCCAGCTGC CTCCACTAAC CTACAAAAAG CACTTTCTAA AGCACTGGAA 
AGCCGGGATG AGTTGAAAAC CAAGTGGGGT GTTTCTGACT TCATCAAGGC CTTTATTAAA 
TTCCACGGTC ATGTATACCT GAGTAAGAGT TTGGAAAAGC TGAGCCCTCT TCGAGAGAAG 
TTAGAAGAAC AGTTTAAGAG ACTGCTGTTC CAAAAAGCTT TCAACTCTCA GCAGTTAGTT 
CATGTCACTG TCATTAACCT GTTTCAACTT CATCATCTTC GTGACTTTAG CAATGAAACA 
GAGCAGCACA GTTACAGCCA AGATGAACAG CTGTGTTGGA CACAGTTGCT GGCCCTCTTC 
ATGTCTTTTC TTGGCATCCT GTGCAAATGT CCTCTCCAGA ATGATTCTCA GGAGTCCTAC 
AATGCCTATC CCCTTCCTGC AGTCAAGGTC TCCATGGACT GGCTAAGACT CCGACCTAGA 
GTCTTTCAAG AAGCTGTAGT GGATGAAAGA CAGTACATCT GGCCCTGGCT AATTTCTCTT 
CTCAATAGTT TTCATCCCCG TGAAGATGAT CTCTCAAGTA CTAATGCCAC ACCACTTCCA 
GAGGAGTTTG AACTACAAGG GTTCCTGGCT TTGAGACCTT CTTTCAGGAA CTTGGATTTT 
TCCAAAGGCC ATCAGGGTAT TACAGGAGAC AAAGAGGGTC AACAACGGCG AATACGGCAG 
CAGCGTTTGA TCTCCATAGG GAAGTGGATT GCTGACAACC AGCCACGGCT GATTCAGTGT 
GAAAATGAGG TAGGGAAATT GTTATTTATC ACAGAAATCC CAGAATTAAT ACTAGAAGAC 
CCCAGTGAAG CCAAAGAGAA CCTCATCCTA CAAGAAACAT CTGCGATAGA GTCACACGCT 
TCGGACGGGA GCCCAGGACT GAAGTCAGTG CTGTCTACAG GCCGAAGTCC AAGCACCAGC 
TGTGACTCAG GAGAGAAGCC AGTGGTCACC TTCAAAGAGA ACATTAAGCC ACGAGAAGTG 
AACAGAGACC AAGGAAGAAG CTTTCCTCCC AAAGAGGTGA GAAGGGACTG TAGCAAAGGA 
GTAACTGTAA CTCAGGATGA CGGACAGAAG GACAGCAGCA AGAGGAGAGC TGAGACCAAG 
AGATGCACCT TAGGAAAGTT GCAGGAAACA GGAAAGCAGA GTGTGGCAGT GCAGGTAAAA 
TCCCAGACAG AACTAAGAAA GACCCCAGTG TCCGAAGCCA GGAAAACTCC TGTCACTCAA 
ACTCCAAGTC AAACAAATAA TTCTCAGTTC ATCCCCATCC ATCACCCTGG AGCCTTCCCT 
CCTCTTCCCA GCCGACCGGG GTTCCCGCCC CCAACATATG TTATCCCCCC TCCTGTGGCA 
TTTTCTATGG GCTCAGGATA CACCTTCCCA GCTGGTGTTT CTGTCCCAGG AACCTTTCTT 
CAGTCTACAG CTCACTCTCC AGCAGGAAAC CAGGTGCAAG CTGGGAAACA GTCCCACATT 
CCTTACAGCC AGCAACGGCC CTCCGGACCA GGGCCAATGA ACCAGGGACC TCAACAATCA 
CAGCCACCTT CCCAGCCACC CCTTACATCT TTACCAGCTC AGCCAACAGC ACAGTCTACA 
AGCCAGTTGC AGGTTCAAGC TCTAGCTCAG CAACAGCAGT CCCCTACAAA AGTCATACCA 
GCCTTGGGGA AAAGCCCGCC TCACCACTCT GGATTCCAGC AGTATCAACA GGCAGATGCC 
TCCAAACAGC TGTGGAATCC CCCTCAGGTT CAAAGCCCAC TAGGGAAAAT TATGCCTGTG 
AAACAGCCCT ACTACCTTCA GACCCAAGAC CCTATAAAAC TGTTTGAGCC GTCATTGCAG 
CCTCCTGTAA TACAGCAACA GCCTCTAGAG AAAAAAATGA AGCCTTTCCC CATGGAGCCA 
TATAACCATA ATCCCTCAGA AGTCAAGGTC CCAGAGTTCT ACTGGGATTC TTCCTACAGC 
ATGGCTGATA ACAGAGCAGT AATGGCTCAG CAACCAAATA TGGACCGCAG GAGCAAACGG 
TCACCCGGAG TCTTCCGTCC AGAGCAGGAT CCTGTGCCCA GGATGCCGTT TGAGGACCCC 
AAGAGCTCCC CTCTGCTTCC TCCGGACCTG TTAAAGAGTC TGGCTGCCTT GGAGGAAGAG 
GAAGAGCTGA TCTTTTCTAA CCCTCCTGAT CTTTACCCAG CTCTGCTGGG TCCTCTCGCC 
TCTCTTCCTG GACGAAGCCT CTTTAAATCC TTACTGGAGA AGCCTTCCGA GCTCATGTCA 
CATTCATCTT CTTTCCTGTC CCTCACTGGG TTCTCTGTCA ATCAGGAAAG ATATCCAAAC 
AGCAGTGTGT TCAATGAAGT GTATGGGAAG AACCTGACAG CCAGCTCCAA GGCAGAGCTG 
AGCCCCTCGG TTGCCTCCTC CCAGGAAACA TCACTGTACT CCCTCTTCGA AGGGACCCCT 
TGGTCTCCAT CACTTCCTGC CAGTTCAGAT CATTCAACAC CAGCCAGCCA GTCTCCTCAT 
TCCTCCAACC CAAGCAGCCT GCCCAGTTCT CCTCCAACAC ATAACCATAA TTCTGCTCCA 
TTCTCTAATT TTGGACCCAT TGGGACTCCA GATAACAGGG ATAGGCGGCC TGCAGATAGG 
TGGAAAACTG ATAAACCAGC CATGGGTGGT TTCGGCGTTG ATTACCTCTC AGCAACGTCA 
TCATCTGAGA ACAGTTGGCA TCAGGCTAGC ACTCCAAGTG GCACCTGGAC AGGCCACGGC 
CCCTCCATGG AGGATTCCTC CGCTGTCCTC ATGGAAAGCC TAAAGTCTAT CTGGTCCAGT 
TCCATGATGC ATCCTGGACC CTCCGCTCTC GAGCAGTTGT TAATGCAGCA GAAGCAGAAA 
CAGCAGCGGG GACAAGGTGC CATGAACCCT CCACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Smg7 ( XM_006250006.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006250068.1 Rattus norvegicus SMG7 nonsense mediated mRNA decay factor (Smg7), transcript variant X2, mRNA.

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CloneID	ORa35453
Clone ID Related Accession (Same CDS sequence)	XM_006250006.3
Accession Version	XM_006250006.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3564bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	protein SMG7 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006250006.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCTGC AGAGCGCGCA GTATCTCCGG CAGGCAGAAG TCCTGAAGGC TGAAATGACA 
GATTCTAAGC TGGGTCCAGC TGAGGTCTGG ACATCCAGGC AAGCTCTGCA GGACTTGTAC 
CAGAAAATGC TGGTTACTGA TCTGGAGTAT GCTTTAGACA AGAAGGTAGA GCAGGACCTC 
TGGAATCATG CCTTTAAGAA TCAGATCACA ACACTACAAG GCCAAGCCAA GAATCGAGCA 
AACCCAAATC GGAGTGAAGT CCAGGCAAAC CTTTCTCTGT TCCTAGAGGC AGCTAGCGGC 
TTCTATACTC AGTTATTACA GGAACTGTGT ACGGTGTTTA ATGTAGATTT GCCATGCCGT 
GTGAAGTCTT CCCAGTTGGG AATTATTAGC AATAAACAGA CGCACACCAG CGCCATAGTG 
AAGCCACAGT CTAGCTCCTG TTCCTACATT TGCCAGCACT GCCTCGTCCA CCTTGGAGAC 
ATTGCTCGAT ACAGAAACCA GACTAGCCAG GCAGAGTCCT ACTATAGGCA TGCAGCTCAG 
CTTGTCCCCT CCAATGGTCA GCCTTACAAT CAGTTGGCTA TCTTAGCTTC TTCCAAAGGA 
GACCACCTGA CCACAATTTT CTACTACTGC AGAAGCATTG CTGTGAAATT CCCTTTCCCA 
GCTGCCTCCA CTAACCTACA AAAAGCACTT TCTAAAGCAC TGGAAAGCCG GGATGAGTTG 
AAAACCAAGT GGGGTGTTTC TGACTTCATC AAGGCCTTTA TTAAATTCCA CGGTCATGTA 
TACCTGAGTA AGAGTTTGGA AAAGCTGAGC CCTCTTCGAG AGAAGTTAGA AGAACAGTTT 
AAGAGACTGC TGTTCCAAAA AGCTTTCAAC TCTCAGCAGT TAGTTCATGT CACTGTCATT 
AACCTGTTTC AACTTCATCA TCTTCGTGAC TTTAGCAATG AAACAGAGCA GCACAGTTAC 
AGCCAAGATG AACAGCTGTG TTGGACACAG TTGCTGGCCC TCTTCATGTC TTTTCTTGGC 
ATCCTGTGCA AATGTCCTCT CCAGAATGAT TCTCAGGAGT CCTACAATGC CTATCCCCTT 
CCTGCAGTCA AGGTCTCCAT GGACTGGCTA AGACTCCGAC CTAGAGTCTT TCAAGAAGCT 
GTAGTGGATG AAAGACAGTA CATCTGGCCC TGGCTAATTT CTCTTCTCAA TAGTTTTCAT 
CCCCGTGAAG ATGATCTCTC AAGTACTAAT GCCACACCAC TTCCAGAGGA GTTTGAACTA 
CAAGGGTTCC TGGCTTTGAG ACCTTCTTTC AGGAACTTGG ATTTTTCCAA AGGCCATCAG 
GGTATTACAG GAGACAAAGA GGGTCAACAA CGGCGAATAC GGCAGCAGCG TTTGATCTCC 
ATAGGGAAGT GGATTGCTGA CAACCAGCCA CGGCTGATTC AGTGTGAAAA TGAGGTAGGG 
AAATTGTTAT TTATCACAGA AATCCCAGAA TTAATACTAG AAGACCCCAG TGAAGCCAAA 
GAGAACCTCA TCCTACAAGA AACATCTGCG ATAGAGTCAC ACGCTTCGGA CGGGAGCCCA 
GGACTGAAGT CAGTGCTGTC TACAGGCCGA AGTCCAAGCA CCAGCTGTGA CTCAGGAGAG 
AAGCCAGTGG TCACCTTCAA AGAGAACATT AAGCCACGAG AAGTGAACAG AGACCAAGGA 
AGAAGCTTTC CTCCCAAAGA GGTGAGAAGG GACTGTAGCA AAGGAGTAAC TGTAACTCAG 
GATGACGGAC AGAAGGACAG CAGCAAGAGG AGAGCTGAGA CCAAGAGATG CACCTTAGGA 
AAGTTGCAGG AAACAGGAAA GCAGAGTGTG GCAGTGCAGG TAAAATCCCA GACAGAACTA 
AGAAAGACCC CAGTGTCCGA AGCCAGGAAA ACTCCTGTCA CTCAAACTCC AAGTCAAACA 
AATAATTCTC AGTTCATCCC CATCCATCAC CCTGGAGCCT TCCCTCCTCT TCCCAGCCGA 
CCGGGGTTCC CGCCCCCAAC ATATGTTATC CCCCCTCCTG TGGCATTTTC TATGGGCTCA 
GGATACACCT TCCCAGCTGG TGTTTCTGTC CCAGGAACCT TTCTTCAGTC TACAGCTCAC 
TCTCCAGCAG GAAACCAGGT GCAAGCTGGG AAACAGTCCC ACATTCCTTA CAGCCAGCAA 
CGGCCCTCCG GACCAGGGCC AATGAACCAG GGACCTCAAC AATCACAGCC ACCTTCCCAG 
CCACCCCTTA CATCTTTACC AGCTCAGCCA ACAGCACAGT CTACAAGCCA GTTGCAGGTT 
CAAGCTCTAG CTCAGCAACA GCAGTCCCCT ACAAAAGTCA TACCAGCCTT GGGGAAAAGC 
CCGCCTCACC ACTCTGGATT CCAGCAGTAT CAACAGGCAG ATGCCTCCAA ACAGCTGTGG 
AATCCCCCTC AGGTTCAAAG CCCACTAGGG AAAATTATGC CTGTGAAACA GCCCTACTAC 
CTTCAGACCC AAGACCCTAT AAAACTGTTT GAGCCGTCAT TGCAGCCTCC TGTAATACAG 
CAACAGCCTC TAGAGAAAAA AATGAAGCCT TTCCCCATGG AGCCATATAA CCATAATCCC 
TCAGAAGTCA AGGTCCCAGA GTTCTACTGG GATTCTTCCT ACAGCATGGC TGATAACAGA 
GCAGTAATGG CTCAGCAACC AAATATGGAC CGCAGGAGCA AACGGTCACC CGGAGTCTTC 
CGTCCAGAGC AGGATCCTGT GCCCAGGATG CCGTTTGAGG ACCCCAAGAG CTCCCCTCTG 
CTTCCTCCGG ACCTGTTAAA GAGTCTGGCT GCCTTGGAGG AAGAGGAAGA GCTGATCTTT 
TCTAACCCTC CTGATCTTTA CCCAGCTCTG CTGGGTCCTC TCGCCTCTCT TCCTGGACGA 
AGCCTCTTTA AATCCTTACT GGAGAAGCCT TCCGAGCTCA TGTCACATTC ATCTTCTTTC 
CTGTCCCTCA CTGGGTTCTC TGTCAATCAG GAAAGATATC CAAACAGCAG TGTGTTCAAT 
GAAGTGTATG GGAAGAACCT GACAGCCAGC TCCAAGGCAG AGCTGAGCCC CTCGGTTGCC 
TCCTCCCAGG AAACATCACT GTACTCCCTC TTCGAAGGGA CCCCTTGGTC TCCATCACTT 
CCTGCCAGTT CAGATCATTC AACACCAGCC AGCCAGTCTC CTCATTCCTC CAACCCAAGC 
AGCCTGCCCA GTTCTCCTCC AACACATAAC CATAATTCTG CTCCATTCTC TAATTTTGGA 
CCCATTGGGA CTCCAGATAA CAGGGATAGG CGGCCTGCAG ATAGGTGGAA AACTGATAAA 
CCAGCCATGG GTGGTTTCGG CGTTGATTAC CTCTCAGCAA CGTCATCATC TGAGAACAGT 
TGGCATCAGG CTAGCACTCC AAGTGGCACC TGGACAGGCC ACGGCCCCTC CATGGAGGAT 
TCCTCCGCTG TCCTCATGGA AAGCCTAAAG TCTATCTGGT CCAGTTCCAT GATGCATCCT 
GGACCCTCCG CTCTCGAGCA GTTGTTAATG CAGCAGAAGC AGAAACAGCA GCGGGGACAA 
GGTGCCATGA ACCCTCCACA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006250068.1
CDS	250..3813
Translation	MSLQSAQYLRQAEVLKAEMTDSKLGPAEVWTSRQALQDLYQKMLVTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRANPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQTHTSAIVKPQSSSCSYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNLQKALSKALESRDELKTKWGVSDFIKAFIKFHGHVYLSKSLEKLSPLREKLEEQFKRLLFQKAFNSQQLVHVTVINLFQLHHLRDFSNETEQHSYSQDEQLCWTQLLALFMSFLGILCKCPLQNDSQESYNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPREDDLSSTNATPLPEEFELQGFLALRPSFRNLDFSKGHQGITGDKEGQQRRIRQQRLISIGKWIADNQPRLIQCENEVGKLLFITEIPELILEDPSEAKENLILQETSAIESHASDGSPGLKSVLSTGRSPSTSCDSGEKPVVTFKENIKPREVNRDQGRSFPPKEVRRDCSKGVTVTQDDGQKDSSKRRAETKRCTLGKLQETGKQSVAVQVKSQTELRKTPVSEARKTPVTQTPSQTNNSQFIPIHHPGAFPPLPSRPGFPPPTYVIPPPVAFSMGSGYTFPAGVSVPGTFLQSTAHSPAGNQVQAGKQSHIPYSQQRPSGPGPMNQGPQQSQPPSQPPLTSLPAQPTAQSTSQLQVQALAQQQQSPTKVIPALGKSPPHHSGFQQYQQADASKQLWNPPQVQSPLGKIMPVKQPYYLQTQDPIKLFEPSLQPPVIQQQPLEKKMKPFPMEPYNHNPSEVKVPEFYWDSSYSMADNRAVMAQQPNMDRRSKRSPGVFRPEQDPVPRMPFEDPKSSPLLPPDLLKSLAALEEEEELIFSNPPDLYPALLGPLASLPGRSLFKSLLEKPSELMSHSSSFLSLTGFSVNQERYPNSSVFNEVYGKNLTASSKAELSPSVASSQETSLYSLFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSAPFSNFGPIGTPDNRDRRPADRWKTDKPAMGGFGVDYLSATSSSENSWHQASTPSGTWTGHGPSMEDSSAVLMESLKSIWSSSMMHPGPSALEQLLMQQKQKQQRGQGAMNPPH

Target ORF information:

RefSeq Version	XM_006250006.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus SMG7 nonsense mediated mRNA decay factor (Smg7), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006250006.3

ORF Insert Sequence:

ATGAGCCTGC AGAGCGCGCA GTATCTCCGG CAGGCAGAAG TCCTGAAGGC TGAAATGACA 
GATTCTAAGC TGGGTCCAGC TGAGGTCTGG ACATCCAGGC AAGCTCTGCA GGACTTGTAC 
CAGAAAATGC TGGTTACTGA TCTGGAGTAT GCTTTAGACA AGAAGGTAGA GCAGGACCTC 
TGGAATCATG CCTTTAAGAA TCAGATCACA ACACTACAAG GCCAAGCCAA GAATCGAGCA 
AACCCAAATC GGAGTGAAGT CCAGGCAAAC CTTTCTCTGT TCCTAGAGGC AGCTAGCGGC 
TTCTATACTC AGTTATTACA GGAACTGTGT ACGGTGTTTA ATGTAGATTT GCCATGCCGT 
GTGAAGTCTT CCCAGTTGGG AATTATTAGC AATAAACAGA CGCACACCAG CGCCATAGTG 
AAGCCACAGT CTAGCTCCTG TTCCTACATT TGCCAGCACT GCCTCGTCCA CCTTGGAGAC 
ATTGCTCGAT ACAGAAACCA GACTAGCCAG GCAGAGTCCT ACTATAGGCA TGCAGCTCAG 
CTTGTCCCCT CCAATGGTCA GCCTTACAAT CAGTTGGCTA TCTTAGCTTC TTCCAAAGGA 
GACCACCTGA CCACAATTTT CTACTACTGC AGAAGCATTG CTGTGAAATT CCCTTTCCCA 
GCTGCCTCCA CTAACCTACA AAAAGCACTT TCTAAAGCAC TGGAAAGCCG GGATGAGTTG 
AAAACCAAGT GGGGTGTTTC TGACTTCATC AAGGCCTTTA TTAAATTCCA CGGTCATGTA 
TACCTGAGTA AGAGTTTGGA AAAGCTGAGC CCTCTTCGAG AGAAGTTAGA AGAACAGTTT 
AAGAGACTGC TGTTCCAAAA AGCTTTCAAC TCTCAGCAGT TAGTTCATGT CACTGTCATT 
AACCTGTTTC AACTTCATCA TCTTCGTGAC TTTAGCAATG AAACAGAGCA GCACAGTTAC 
AGCCAAGATG AACAGCTGTG TTGGACACAG TTGCTGGCCC TCTTCATGTC TTTTCTTGGC 
ATCCTGTGCA AATGTCCTCT CCAGAATGAT TCTCAGGAGT CCTACAATGC CTATCCCCTT 
CCTGCAGTCA AGGTCTCCAT GGACTGGCTA AGACTCCGAC CTAGAGTCTT TCAAGAAGCT 
GTAGTGGATG AAAGACAGTA CATCTGGCCC TGGCTAATTT CTCTTCTCAA TAGTTTTCAT 
CCCCGTGAAG ATGATCTCTC AAGTACTAAT GCCACACCAC TTCCAGAGGA GTTTGAACTA 
CAAGGGTTCC TGGCTTTGAG ACCTTCTTTC AGGAACTTGG ATTTTTCCAA AGGCCATCAG 
GGTATTACAG GAGACAAAGA GGGTCAACAA CGGCGAATAC GGCAGCAGCG TTTGATCTCC 
ATAGGGAAGT GGATTGCTGA CAACCAGCCA CGGCTGATTC AGTGTGAAAA TGAGGTAGGG 
AAATTGTTAT TTATCACAGA AATCCCAGAA TTAATACTAG AAGACCCCAG TGAAGCCAAA 
GAGAACCTCA TCCTACAAGA AACATCTGCG ATAGAGTCAC ACGCTTCGGA CGGGAGCCCA 
GGACTGAAGT CAGTGCTGTC TACAGGCCGA AGTCCAAGCA CCAGCTGTGA CTCAGGAGAG 
AAGCCAGTGG TCACCTTCAA AGAGAACATT AAGCCACGAG AAGTGAACAG AGACCAAGGA 
AGAAGCTTTC CTCCCAAAGA GGTGAGAAGG GACTGTAGCA AAGGAGTAAC TGTAACTCAG 
GATGACGGAC AGAAGGACAG CAGCAAGAGG AGAGCTGAGA CCAAGAGATG CACCTTAGGA 
AAGTTGCAGG AAACAGGAAA GCAGAGTGTG GCAGTGCAGG TAAAATCCCA GACAGAACTA 
AGAAAGACCC CAGTGTCCGA AGCCAGGAAA ACTCCTGTCA CTCAAACTCC AAGTCAAACA 
AATAATTCTC AGTTCATCCC CATCCATCAC CCTGGAGCCT TCCCTCCTCT TCCCAGCCGA 
CCGGGGTTCC CGCCCCCAAC ATATGTTATC CCCCCTCCTG TGGCATTTTC TATGGGCTCA 
GGATACACCT TCCCAGCTGG TGTTTCTGTC CCAGGAACCT TTCTTCAGTC TACAGCTCAC 
TCTCCAGCAG GAAACCAGGT GCAAGCTGGG AAACAGTCCC ACATTCCTTA CAGCCAGCAA 
CGGCCCTCCG GACCAGGGCC AATGAACCAG GGACCTCAAC AATCACAGCC ACCTTCCCAG 
CCACCCCTTA CATCTTTACC AGCTCAGCCA ACAGCACAGT CTACAAGCCA GTTGCAGGTT 
CAAGCTCTAG CTCAGCAACA GCAGTCCCCT ACAAAAGTCA TACCAGCCTT GGGGAAAAGC 
CCGCCTCACC ACTCTGGATT CCAGCAGTAT CAACAGGCAG ATGCCTCCAA ACAGCTGTGG 
AATCCCCCTC AGGTTCAAAG CCCACTAGGG AAAATTATGC CTGTGAAACA GCCCTACTAC 
CTTCAGACCC AAGACCCTAT AAAACTGTTT GAGCCGTCAT TGCAGCCTCC TGTAATACAG 
CAACAGCCTC TAGAGAAAAA AATGAAGCCT TTCCCCATGG AGCCATATAA CCATAATCCC 
TCAGAAGTCA AGGTCCCAGA GTTCTACTGG GATTCTTCCT ACAGCATGGC TGATAACAGA 
GCAGTAATGG CTCAGCAACC AAATATGGAC CGCAGGAGCA AACGGTCACC CGGAGTCTTC 
CGTCCAGAGC AGGATCCTGT GCCCAGGATG CCGTTTGAGG ACCCCAAGAG CTCCCCTCTG 
CTTCCTCCGG ACCTGTTAAA GAGTCTGGCT GCCTTGGAGG AAGAGGAAGA GCTGATCTTT 
TCTAACCCTC CTGATCTTTA CCCAGCTCTG CTGGGTCCTC TCGCCTCTCT TCCTGGACGA 
AGCCTCTTTA AATCCTTACT GGAGAAGCCT TCCGAGCTCA TGTCACATTC ATCTTCTTTC 
CTGTCCCTCA CTGGGTTCTC TGTCAATCAG GAAAGATATC CAAACAGCAG TGTGTTCAAT 
GAAGTGTATG GGAAGAACCT GACAGCCAGC TCCAAGGCAG AGCTGAGCCC CTCGGTTGCC 
TCCTCCCAGG AAACATCACT GTACTCCCTC TTCGAAGGGA CCCCTTGGTC TCCATCACTT 
CCTGCCAGTT CAGATCATTC AACACCAGCC AGCCAGTCTC CTCATTCCTC CAACCCAAGC 
AGCCTGCCCA GTTCTCCTCC AACACATAAC CATAATTCTG CTCCATTCTC TAATTTTGGA 
CCCATTGGGA CTCCAGATAA CAGGGATAGG CGGCCTGCAG ATAGGTGGAA AACTGATAAA 
CCAGCCATGG GTGGTTTCGG CGTTGATTAC CTCTCAGCAA CGTCATCATC TGAGAACAGT 
TGGCATCAGG CTAGCACTCC AAGTGGCACC TGGACAGGCC ACGGCCCCTC CATGGAGGAT 
TCCTCCGCTG TCCTCATGGA AAGCCTAAAG TCTATCTGGT CCAGTTCCAT GATGCATCCT 
GGACCCTCCG CTCTCGAGCA GTTGTTAATG CAGCAGAAGC AGAAACAGCA GCGGGGACAA 
GGTGCCATGA ACCCTCCACA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Smg7 ( XM_008769655.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008767877.1 Rattus norvegicus SMG7 nonsense mediated mRNA decay factor (Smg7), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa35455
Clone ID Related Accession (Same CDS sequence)	XM_008769655.2
Accession Version	XM_008769655.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3489bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	protein SMG7 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008769655.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTTAC AGATAGTCAT TGTGGGAGAA AACGTTTCAT TTAAGTCCCA GATGAGGACC 
GAAAACTTGA AATCAGAGGA GCATCTGAAA TCAAGTAATA TTAGGCAGGC AGAAGTCCTG 
AAGGCTGAAA TGACAGATTC TAAGCTGGGT CCAGCTGAGG TCTGGACATC CAGGCAAGCT 
CTGCAGGACT TGTACCAGAA AATGCTGGTT ACTGATCTGG AGTATGCTTT AGACAAGAAG 
GTAGAGCAGG ACCTCTGGAA TCATGCCTTT AAGAATCAGA TCACAACACT ACAAGGCCAA 
GCCAAGAATC GAGCAAACCC AAATCGGAGT GAAGTCCAGG CAAACCTTTC TCTGTTCCTA 
GAGGCAGCTA GCGGCTTCTA TACTCAGTTA TTACAGGAAC TGTGTACGGT GTTTAATGTA 
GATTTGCCAT GCCGTGTGAA GTCTTCCCAG TTGGGAATTA TTAGCAATAA ACAGACGCAC 
ACCAGCGCCA TAGTGAAGCC ACAGTCTAGC TCCTGTTCCT ACATTTGCCA GCACTGCCTC 
GTCCACCTTG GAGACATTGC TCGATACAGA AACCAGACTA GCCAGGCAGA GTCCTACTAT 
AGGCATGCAG CTCAGCTTGT CCCCTCCAAT GGTCAGCCTT ACAATCAGTT GGCTATCTTA 
GCTTCTTCCA AAGGAGACCA CCTGACCACA ATTTTCTACT ACTGCAGAAG CATTGCTGTG 
AAATTCCCTT TCCCAGCTGC CTCCACTAAC CTACAAAAAG CACTTTCTAA AGCACTGGAA 
AGCCGGGATG AGTTGAAAAC CAAGTGGGGT GTTTCTGACT TCATCAAGGC CTTTATTAAA 
TTCCACGGTC ATGTATACCT GAGTAAGAGT TTGGAAAAGC TGAGCCCTCT TCGAGAGAAG 
TTAGAAGAAC AGTTTAAGAG ACTGCTGTTC CAAAAAGCTT TCAACTCTCA GCAGTTAGTT 
CATGTCACTG TCATTAACCT GTTTCAACTT CATCATCTTC GTGACTTTAG CAATGAAACA 
GAGCAGCACA GTTACAGCCA AGATGAACAG CTGTGTTGGA CACAGTTGCT GGCCCTCTTC 
ATGTCTTTTC TTGGCATCCT GTGCAAATGT CCTCTCCAGA ATGATTCTCA GGAGTCCTAC 
AATGCCTATC CCCTTCCTGC AGTCAAGGTC TCCATGGACT GGCTAAGACT CCGACCTAGA 
GTCTTTCAAG AAGCTGTAGT GGATGAAAGA CAGTACATCT GGCCCTGGCT AATTTCTCTT 
CTCAATAGTT TTCATCCCCG TGAAGATGAT CTCTCAAGTA CTAATGCCAC ACCACTTCCA 
GAGGAGTTTG AACTACAAGG GTTCCTGGCT TTGAGACCTT CTTTCAGGAA CTTGGATTTT 
TCCAAAGGCC ATCAGGGTAT TACAGGAGAC AAAGAGGGTC AACAACGGCG AATACGGCAG 
CAGCGTTTGA TCTCCATAGG GAAGTGGATT GCTGACAACC AGCCACGGCT GATTCAGTGT 
GAAAATGAGG TAGGGAAATT GTTATTTATC ACAGAAATCC CAGAATTAAT ACTAGAAGAC 
CCCAGTGAAG CCAAAGAGAA CCTCATCCTA CAAGAAACAT CTGCGATAGA GTCACACGCT 
TCGGACGGGA GCCCAGGACT GAAGTCAGTG CTGTCTACAG GCCGAAGTCC AAGCACCAGC 
TGTGACTCAG GAGAGAAGCC AGTGGTCACC TTCAAAGAGA ACATTAAGCC ACGAGAAGTG 
AACAGAGACC AAGGAAGAAG CTTTCCTCCC AAAGAGGTGA GAAGGGACTG TAGCAAAGGA 
GTAACTGTAA CTCAGGATGA CGGACAGAAG GACAGCAGCA AGAGGAGAGC TGAGACCAAG 
AGATGCACCT TAGGAAAGTT GCAGGAAACA GGAAAGCAGA GTGTGGCAGT GCAGGTAAAA 
TCCCAGACAG AACTAAGAAA GACCCCAGTG TCCGAAGCCA GGAAAACTCC TGTCACTCAA 
ACTCCAAGTC AAACAAATAA TTCTCAGTTC ATCCCCATCC ATCACCCTGG AGCCTTCCCT 
CCTCTTCCCA GCCGACCGGG GTTCCCGCCC CCAACATATG TTATCCCCCC TCCTGTGGCA 
TTTTCTATGG GCTCAGGATA CACCTTCCCA GCTGGTGTTT CTGTCCCAGG AACCTTTCTT 
CAGTCTACAG CTCACTCTCC AGCAGGAAAC CAGGTGCAAG CTGGGAAACA GTCCCACATT 
CCTTACAGCC AGCAACGGCC CTCCGGACCA GGGCCAATGA ACCAGGGACC TCAACAATCA 
CAGCCACCTT CCCAGCCACC CCTTACATCT TTACCAGCTC AGCCAACAGC ACAGTCTACA 
AGCCAGTTGC AGGTTCAAGC TCTAGCTCAG CAACAGCAGT CCCCTACAAA AGTCATACCA 
GCCTTGGGGA AAAGCCCGCC TCACCACTCT GGATTCCAGC AGTATCAACA GGCAGATGCC 
TCCAAACAGC TGTGGAATCC CCCTCAGGTT CAAAGCCCAC TAGGGAAAAT TATGCCTGTG 
AAACAGCCCT ACTACCTTCA GACCCAAGAC CCTATAAAAC TGTTTGAGCC GTCATTGCAG 
CCTCCTGTAA TACAGCAACA GCCTCTAGAG AAAAAAATGA AGCCTTTCCC CATGGAGCCA 
TATAACCATA ATCCCTCAGA AGTCAAGGTC CCAGAGTTCT ACTGGGATTC TTCCTACAGC 
ATGGCTGATA ACAGAGCAGT AATGGCTCAG CAACCAAATA TGGACCGCAG GAGCAAACGG 
TCACCCGGAG TCTTCCGTCC AGAGCAGGAT CCTGTGCCCA GGATGCCGTT TGAGAAATCC 
TTACTGGAGA AGCCTTCCGA GCTCATGTCA CATTCATCTT CTTTCCTGTC CCTCACTGGG 
TTCTCTGTCA ATCAGGAAAG ATATCCAAAC AGCAGTGTGT TCAATGAAGT GTATGGGAAG 
AACCTGACAG CCAGCTCCAA GGCAGAGCTG AGCCCCTCGG TTGCCTCCTC CCAGGAAACA 
TCACTGTACT CCCTCTTCGA AGGGACCCCT TGGTCTCCAT CACTTCCTGC CAGTTCAGAT 
CATTCAACAC CAGCCAGCCA GTCTCCTCAT TCCTCCAACC CAAGCAGCCT GCCCAGTTCT 
CCTCCAACAC ATAACCATAA TTCTGCTCCA TTCTCTAATT TTGGACCCAT TGGGACTCCA 
GATAACAGGG ATAGGCGGCC TGCAGATAGG TGGAAAACTG ATAAACCAGC CATGGGTGGT 
TTCGGCGTTG ATTACCTCTC AGCAACGTCA TCATCTGAGA ACAGTTGGCA TCAGGCTAGC 
ACTCCAAGTG GCACCTGGAC AGGCCACGGC CCCTCCATGG AGGATTCCTC CGCTGTCCTC 
ATGGAAAGCC TAAAGTCTAT CTGGTCCAGT TCCATGATGC ATCCTGGACC CTCCGCTCTC 
GAGCAGTTGT TAATGCAGCA GAAGCAGAAA CAGCAGCGGG GACAAGGTGC CATGAACCCT 
CCACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008767877.1
CDS	1404..4892
Translation	MLLQIVIVGENVSFKSQMRTENLKSEEHLKSSNIRQAEVLKAEMTDSKLGPAEVWTSRQALQDLYQKMLVTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRANPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQTHTSAIVKPQSSSCSYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNLQKALSKALESRDELKTKWGVSDFIKAFIKFHGHVYLSKSLEKLSPLREKLEEQFKRLLFQKAFNSQQLVHVTVINLFQLHHLRDFSNETEQHSYSQDEQLCWTQLLALFMSFLGILCKCPLQNDSQESYNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPREDDLSSTNATPLPEEFELQGFLALRPSFRNLDFSKGHQGITGDKEGQQRRIRQQRLISIGKWIADNQPRLIQCENEVGKLLFITEIPELILEDPSEAKENLILQETSAIESHASDGSPGLKSVLSTGRSPSTSCDSGEKPVVTFKENIKPREVNRDQGRSFPPKEVRRDCSKGVTVTQDDGQKDSSKRRAETKRCTLGKLQETGKQSVAVQVKSQTELRKTPVSEARKTPVTQTPSQTNNSQFIPIHHPGAFPPLPSRPGFPPPTYVIPPPVAFSMGSGYTFPAGVSVPGTFLQSTAHSPAGNQVQAGKQSHIPYSQQRPSGPGPMNQGPQQSQPPSQPPLTSLPAQPTAQSTSQLQVQALAQQQQSPTKVIPALGKSPPHHSGFQQYQQADASKQLWNPPQVQSPLGKIMPVKQPYYLQTQDPIKLFEPSLQPPVIQQQPLEKKMKPFPMEPYNHNPSEVKVPEFYWDSSYSMADNRAVMAQQPNMDRRSKRSPGVFRPEQDPVPRMPFEKSLLEKPSELMSHSSSFLSLTGFSVNQERYPNSSVFNEVYGKNLTASSKAELSPSVASSQETSLYSLFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSAPFSNFGPIGTPDNRDRRPADRWKTDKPAMGGFGVDYLSATSSSENSWHQASTPSGTWTGHGPSMEDSSAVLMESLKSIWSSSMMHPGPSALEQLLMQQKQKQQRGQGAMNPPH

Target ORF information:

RefSeq Version	XM_008769655.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus SMG7 nonsense mediated mRNA decay factor (Smg7), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008769655.2

ORF Insert Sequence:

ATGCTCTTAC AGATAGTCAT TGTGGGAGAA AACGTTTCAT TTAAGTCCCA GATGAGGACC 
GAAAACTTGA AATCAGAGGA GCATCTGAAA TCAAGTAATA TTAGGCAGGC AGAAGTCCTG 
AAGGCTGAAA TGACAGATTC TAAGCTGGGT CCAGCTGAGG TCTGGACATC CAGGCAAGCT 
CTGCAGGACT TGTACCAGAA AATGCTGGTT ACTGATCTGG AGTATGCTTT AGACAAGAAG 
GTAGAGCAGG ACCTCTGGAA TCATGCCTTT AAGAATCAGA TCACAACACT ACAAGGCCAA 
GCCAAGAATC GAGCAAACCC AAATCGGAGT GAAGTCCAGG CAAACCTTTC TCTGTTCCTA 
GAGGCAGCTA GCGGCTTCTA TACTCAGTTA TTACAGGAAC TGTGTACGGT GTTTAATGTA 
GATTTGCCAT GCCGTGTGAA GTCTTCCCAG TTGGGAATTA TTAGCAATAA ACAGACGCAC 
ACCAGCGCCA TAGTGAAGCC ACAGTCTAGC TCCTGTTCCT ACATTTGCCA GCACTGCCTC 
GTCCACCTTG GAGACATTGC TCGATACAGA AACCAGACTA GCCAGGCAGA GTCCTACTAT 
AGGCATGCAG CTCAGCTTGT CCCCTCCAAT GGTCAGCCTT ACAATCAGTT GGCTATCTTA 
GCTTCTTCCA AAGGAGACCA CCTGACCACA ATTTTCTACT ACTGCAGAAG CATTGCTGTG 
AAATTCCCTT TCCCAGCTGC CTCCACTAAC CTACAAAAAG CACTTTCTAA AGCACTGGAA 
AGCCGGGATG AGTTGAAAAC CAAGTGGGGT GTTTCTGACT TCATCAAGGC CTTTATTAAA 
TTCCACGGTC ATGTATACCT GAGTAAGAGT TTGGAAAAGC TGAGCCCTCT TCGAGAGAAG 
TTAGAAGAAC AGTTTAAGAG ACTGCTGTTC CAAAAAGCTT TCAACTCTCA GCAGTTAGTT 
CATGTCACTG TCATTAACCT GTTTCAACTT CATCATCTTC GTGACTTTAG CAATGAAACA 
GAGCAGCACA GTTACAGCCA AGATGAACAG CTGTGTTGGA CACAGTTGCT GGCCCTCTTC 
ATGTCTTTTC TTGGCATCCT GTGCAAATGT CCTCTCCAGA ATGATTCTCA GGAGTCCTAC 
AATGCCTATC CCCTTCCTGC AGTCAAGGTC TCCATGGACT GGCTAAGACT CCGACCTAGA 
GTCTTTCAAG AAGCTGTAGT GGATGAAAGA CAGTACATCT GGCCCTGGCT AATTTCTCTT 
CTCAATAGTT TTCATCCCCG TGAAGATGAT CTCTCAAGTA CTAATGCCAC ACCACTTCCA 
GAGGAGTTTG AACTACAAGG GTTCCTGGCT TTGAGACCTT CTTTCAGGAA CTTGGATTTT 
TCCAAAGGCC ATCAGGGTAT TACAGGAGAC AAAGAGGGTC AACAACGGCG AATACGGCAG 
CAGCGTTTGA TCTCCATAGG GAAGTGGATT GCTGACAACC AGCCACGGCT GATTCAGTGT 
GAAAATGAGG TAGGGAAATT GTTATTTATC ACAGAAATCC CAGAATTAAT ACTAGAAGAC 
CCCAGTGAAG CCAAAGAGAA CCTCATCCTA CAAGAAACAT CTGCGATAGA GTCACACGCT 
TCGGACGGGA GCCCAGGACT GAAGTCAGTG CTGTCTACAG GCCGAAGTCC AAGCACCAGC 
TGTGACTCAG GAGAGAAGCC AGTGGTCACC TTCAAAGAGA ACATTAAGCC ACGAGAAGTG 
AACAGAGACC AAGGAAGAAG CTTTCCTCCC AAAGAGGTGA GAAGGGACTG TAGCAAAGGA 
GTAACTGTAA CTCAGGATGA CGGACAGAAG GACAGCAGCA AGAGGAGAGC TGAGACCAAG 
AGATGCACCT TAGGAAAGTT GCAGGAAACA GGAAAGCAGA GTGTGGCAGT GCAGGTAAAA 
TCCCAGACAG AACTAAGAAA GACCCCAGTG TCCGAAGCCA GGAAAACTCC TGTCACTCAA 
ACTCCAAGTC AAACAAATAA TTCTCAGTTC ATCCCCATCC ATCACCCTGG AGCCTTCCCT 
CCTCTTCCCA GCCGACCGGG GTTCCCGCCC CCAACATATG TTATCCCCCC TCCTGTGGCA 
TTTTCTATGG GCTCAGGATA CACCTTCCCA GCTGGTGTTT CTGTCCCAGG AACCTTTCTT 
CAGTCTACAG CTCACTCTCC AGCAGGAAAC CAGGTGCAAG CTGGGAAACA GTCCCACATT 
CCTTACAGCC AGCAACGGCC CTCCGGACCA GGGCCAATGA ACCAGGGACC TCAACAATCA 
CAGCCACCTT CCCAGCCACC CCTTACATCT TTACCAGCTC AGCCAACAGC ACAGTCTACA 
AGCCAGTTGC AGGTTCAAGC TCTAGCTCAG CAACAGCAGT CCCCTACAAA AGTCATACCA 
GCCTTGGGGA AAAGCCCGCC TCACCACTCT GGATTCCAGC AGTATCAACA GGCAGATGCC 
TCCAAACAGC TGTGGAATCC CCCTCAGGTT CAAAGCCCAC TAGGGAAAAT TATGCCTGTG 
AAACAGCCCT ACTACCTTCA GACCCAAGAC CCTATAAAAC TGTTTGAGCC GTCATTGCAG 
CCTCCTGTAA TACAGCAACA GCCTCTAGAG AAAAAAATGA AGCCTTTCCC CATGGAGCCA 
TATAACCATA ATCCCTCAGA AGTCAAGGTC CCAGAGTTCT ACTGGGATTC TTCCTACAGC 
ATGGCTGATA ACAGAGCAGT AATGGCTCAG CAACCAAATA TGGACCGCAG GAGCAAACGG 
TCACCCGGAG TCTTCCGTCC AGAGCAGGAT CCTGTGCCCA GGATGCCGTT TGAGAAATCC 
TTACTGGAGA AGCCTTCCGA GCTCATGTCA CATTCATCTT CTTTCCTGTC CCTCACTGGG 
TTCTCTGTCA ATCAGGAAAG ATATCCAAAC AGCAGTGTGT TCAATGAAGT GTATGGGAAG 
AACCTGACAG CCAGCTCCAA GGCAGAGCTG AGCCCCTCGG TTGCCTCCTC CCAGGAAACA 
TCACTGTACT CCCTCTTCGA AGGGACCCCT TGGTCTCCAT CACTTCCTGC CAGTTCAGAT 
CATTCAACAC CAGCCAGCCA GTCTCCTCAT TCCTCCAACC CAAGCAGCCT GCCCAGTTCT 
CCTCCAACAC ATAACCATAA TTCTGCTCCA TTCTCTAATT TTGGACCCAT TGGGACTCCA 
GATAACAGGG ATAGGCGGCC TGCAGATAGG TGGAAAACTG ATAAACCAGC CATGGGTGGT 
TTCGGCGTTG ATTACCTCTC AGCAACGTCA TCATCTGAGA ACAGTTGGCA TCAGGCTAGC 
ACTCCAAGTG GCACCTGGAC AGGCCACGGC CCCTCCATGG AGGATTCCTC CGCTGTCCTC 
ATGGAAAGCC TAAAGTCTAT CTGGTCCAGT TCCATGATGC ATCCTGGACC CTCCGCTCTC 
GAGCAGTTGT TAATGCAGCA GAAGCAGAAA CAGCAGCGGG GACAAGGTGC CATGAACCCT 
CCACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Smg7 ( XM_008769656.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008767878.1 Rattus norvegicus SMG7 nonsense mediated mRNA decay factor (Smg7), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa35456
Clone ID Related Accession (Same CDS sequence)	XM_008769656.2
Accession Version	XM_008769656.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	protein SMG7 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008769656.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTTAC AGATAGTCAT TGTGGGAGAA AACGTTTCAT TTAAGTCCCA GATGAGGACC 
GAAAACTTGA AATCAGAGGA GCATCTGAAA TCAAGTAATA TTAGGCAGGC AGAAGTCCTG 
AAGGCTGAAA TGACAGATTC TAAGCTGGGT CCAGCTGAGG TCTGGACATC CAGGCAAGCT 
CTGCAGGACT TGTACCAGAA AATGCTGGTT ACTGATCTGG AGTATGCTTT AGACAAGAAG 
GTAGAGCAGG ACCTCTGGAA TCATGCCTTT AAGAATCAGA TCACAACACT ACAAGGCCAA 
GCCAAGAATC GAGCAAACCC AAATCGGAGT GAAGTCCAGG CAAACCTTTC TCTGTTCCTA 
GAGGCAGCTA GCGGCTTCTA TACTCAGTTA TTACAGGAAC TGTGTACGGT GTTTAATGTA 
GATTTGCCAT GCCGTGTGAA GTCTTCCCAG TTGGGAATTA TTAGCAATAA ACAGACGCAC 
ACCAGCGCCA TAGTGAAGCC ACAGTCTAGC TCCTGTTCCT ACATTTGCCA GCACTGCCTC 
GTCCACCTTG GAGACATTGC TCGATACAGA AACCAGACTA GCCAGGCAGA GTCCTACTAT 
AGGCATGCAG CTCAGCTTGT CCCCTCCAAT GGTCAGCCTT ACAATCAGTT GGCTATCTTA 
GCTTCTTCCA AAGGAGACCA CCTGACCACA ATTTTCTACT ACTGCAGAAG CATTGCTGTG 
AAATTCCCTT TCCCAGCTGC CTCCACTAAC CTACAAAAAG CACTTTCTAA AGCACTGGAA 
AGCCGGGATG AGTTGAAAAC CAAGTGGGGT GTTTCTGACT TCATCAAGGC CTTTATTAAA 
TTCCACGGTC ATGTATACCT GAGTAAGAGT TTGGAAAAGC TGAGCCCTCT TCGAGAGAAG 
TTAGAAGAAC AGTTTAAGAG ACTGCTGTTC CAAAAAGCTT TCAACTCTCA GCAGTTAGTT 
CATGTCACTG TCATTAACCT GTTTCAACTT CATCATCTTC GTGACTTTAG CAATGAAACA 
GAGCAGCACA GTTACAGCCA AGATGAACAG CTGTGTTGGA CACAGTTGCT GGCCCTCTTC 
ATGTCTTTTC TTGGCATCCT GTGCAAATGT CCTCTCCAGA ATGATTCTCA GGAGTCCTAC 
AATGCCTATC CCCTTCCTGC AGTCAAGGTC TCCATGGACT GGCTAAGACT CCGACCTAGA 
GTCTTTCAAG AAGCTGTAGT GGATGAAAGA CAGTACATCT GGCCCTGGCT AATTTCTCTT 
CTCAATAGTT TTCATCCCCG TGAAGATGAT CTCTCAAGTA CTAATGCCAC ACCACTTCCA 
GAGGAGTTTG AACTACAAGG GTTCCTGGCT TTGAGACCTT CTTTCAGGAA CTTGGATTTT 
TCCAAAGGCC ATCAGGGTAT TACAGGAGAC AAAGAGGGTC AACAACGGCG AATACGGCAG 
CAGCGTTTGA TCTCCATAGG GAAGTGGATT GCTGACAACC AGCCACGGCT GATTCAGTGT 
GAAAATGAGG TAGGGAAATT GTTATTTATC ACAGAAATCC CAGAATTAAT ACTAGAAGAC 
CCCAGTGAAG CCAAAGAGAA CCTCATCCTA CAAGAAACAT CTGCGATAGA GTCACACGCT 
TCGGACGGGA GCCCAGGACT GAAGTCAGTG CTGTCTACAG GCCGAAGTCC AAGCACCAGC 
TGTGACTCAG GAGAGAAGCC AGTGGTCACC TTCAAAGAGA ACATTAAGCC ACGAGAAGTG 
AACAGAGACC AAGGAAGAAG CTTTCCTCCC AAAGAGGTAA AATCCCAGAC AGAACTAAGA 
AAGACCCCAG TGTCCGAAGC CAGGAAAACT CCTGTCACTC AAACTCCAAG TCAAACAAAT 
AATTCTCAGT TCATCCCCAT CCATCACCCT GGAGCCTTCC CTCCTCTTCC CAGCCGACCG 
GGGTTCCCGC CCCCAACATA TGTTATCCCC CCTCCTGTGG CATTTTCTAT GGGCTCAGGA 
TACACCTTCC CAGCTGGTGT TTCTGTCCCA GGAACCTTTC TTCAGTCTAC AGCTCACTCT 
CCAGCAGGAA ACCAGGTGCA AGCTGGGAAA CAGTCCCACA TTCCTTACAG CCAGCAACGG 
CCCTCCGGAC CAGGGCCAAT GAACCAGGGA CCTCAACAAT CACAGCCACC TTCCCAGCCA 
CCCCTTACAT CTTTACCAGC TCAGCCAACA GCACAGTCTA CAAGCCAGTT GCAGGTTCAA 
GCTCTAGCTC AGCAACAGCA GTCCCCTACA AAAGTCATAC CAGCCTTGGG GAAAAGCCCG 
CCTCACCACT CTGGATTCCA GCAGTATCAA CAGGCAGATG CCTCCAAACA GCTGTGGAAT 
CCCCCTCAGG TTCAAAGCCC ACTAGGGAAA ATTATGCCTG TGAAACAGCC CTACTACCTT 
CAGACCCAAG ACCCTATAAA ACTGTTTGAG CCGTCATTGC AGCCTCCTGT AATACAGCAA 
CAGCCTCTAG AGAAAAAAAT GAAGCCTTTC CCCATGGAGC CATATAACCA TAATCCCTCA 
GAAGTCAAGG TCCCAGAGTT CTACTGGGAT TCTTCCTACA GCATGGCTGA TAACAGAGCA 
GTAATGGCTC AGCAACCAAA TATGGACCGC AGGAGCAAAC GGTCACCCGG AGTCTTCCGT 
CCAGAGCAGG ATCCTGTGCC CAGGATGCCG TTTGAGAAAT CCTTACTGGA GAAGCCTTCC 
GAGCTCATGT CACATTCATC TTCTTTCCTG TCCCTCACTG GGTTCTCTGT CAATCAGGAA 
AGATATCCAA ACAGCAGTGT GTTCAATGAA GTGTATGGGA AGAACCTGAC AGCCAGCTCC 
AAGGCAGAGC TGAGCCCCTC GGTTGCCTCC TCCCAGGAAA CATCACTGTA CTCCCTCTTC 
GAAGGGACCC CTTGGTCTCC ATCACTTCCT GCCAGTTCAG ATCATTCAAC ACCAGCCAGC 
CAGTCTCCTC ATTCCTCCAA CCCAAGCAGC CTGCCCAGTT CTCCTCCAAC ACATAACCAT 
AATTCTGCTC CATTCTCTAA TTTTGGACCC ATTGGGACTC CAGATAACAG GGATAGGCGG 
CCTGCAGATA GGTGGAAAAC TGATAAACCA GCCATGGGTG GTTTCGGCGT TGATTACCTC 
TCAGCAACGT CATCATCTGA GAACAGTTGG CATCAGGCTA GCACTCCAAG TGGCACCTGG 
ACAGGCCACG GCCCCTCCAT GGAGGATTCC TCCGCTGTCC TCATGGAAAG CCTAAAGTCT 
ATCTGGTCCA GTTCCATGAT GCATCCTGGA CCCTCCGCTC TCGAGCAGTT GTTAATGCAG 
CAGAAGCAGA AACAGCAGCG GGGACAAGGT GCCATGAACC CTCCACACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008767878.1
CDS	1404..4754
Translation	MLLQIVIVGENVSFKSQMRTENLKSEEHLKSSNIRQAEVLKAEMTDSKLGPAEVWTSRQALQDLYQKMLVTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRANPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQTHTSAIVKPQSSSCSYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNLQKALSKALESRDELKTKWGVSDFIKAFIKFHGHVYLSKSLEKLSPLREKLEEQFKRLLFQKAFNSQQLVHVTVINLFQLHHLRDFSNETEQHSYSQDEQLCWTQLLALFMSFLGILCKCPLQNDSQESYNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPREDDLSSTNATPLPEEFELQGFLALRPSFRNLDFSKGHQGITGDKEGQQRRIRQQRLISIGKWIADNQPRLIQCENEVGKLLFITEIPELILEDPSEAKENLILQETSAIESHASDGSPGLKSVLSTGRSPSTSCDSGEKPVVTFKENIKPREVNRDQGRSFPPKEVKSQTELRKTPVSEARKTPVTQTPSQTNNSQFIPIHHPGAFPPLPSRPGFPPPTYVIPPPVAFSMGSGYTFPAGVSVPGTFLQSTAHSPAGNQVQAGKQSHIPYSQQRPSGPGPMNQGPQQSQPPSQPPLTSLPAQPTAQSTSQLQVQALAQQQQSPTKVIPALGKSPPHHSGFQQYQQADASKQLWNPPQVQSPLGKIMPVKQPYYLQTQDPIKLFEPSLQPPVIQQQPLEKKMKPFPMEPYNHNPSEVKVPEFYWDSSYSMADNRAVMAQQPNMDRRSKRSPGVFRPEQDPVPRMPFEKSLLEKPSELMSHSSSFLSLTGFSVNQERYPNSSVFNEVYGKNLTASSKAELSPSVASSQETSLYSLFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSAPFSNFGPIGTPDNRDRRPADRWKTDKPAMGGFGVDYLSATSSSENSWHQASTPSGTWTGHGPSMEDSSAVLMESLKSIWSSSMMHPGPSALEQLLMQQKQKQQRGQGAMNPPH

Target ORF information:

RefSeq Version	XM_008769656.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus SMG7 nonsense mediated mRNA decay factor (Smg7), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008769656.2

ORF Insert Sequence:

ATGCTCTTAC AGATAGTCAT TGTGGGAGAA AACGTTTCAT TTAAGTCCCA GATGAGGACC 
GAAAACTTGA AATCAGAGGA GCATCTGAAA TCAAGTAATA TTAGGCAGGC AGAAGTCCTG 
AAGGCTGAAA TGACAGATTC TAAGCTGGGT CCAGCTGAGG TCTGGACATC CAGGCAAGCT 
CTGCAGGACT TGTACCAGAA AATGCTGGTT ACTGATCTGG AGTATGCTTT AGACAAGAAG 
GTAGAGCAGG ACCTCTGGAA TCATGCCTTT AAGAATCAGA TCACAACACT ACAAGGCCAA 
GCCAAGAATC GAGCAAACCC AAATCGGAGT GAAGTCCAGG CAAACCTTTC TCTGTTCCTA 
GAGGCAGCTA GCGGCTTCTA TACTCAGTTA TTACAGGAAC TGTGTACGGT GTTTAATGTA 
GATTTGCCAT GCCGTGTGAA GTCTTCCCAG TTGGGAATTA TTAGCAATAA ACAGACGCAC 
ACCAGCGCCA TAGTGAAGCC ACAGTCTAGC TCCTGTTCCT ACATTTGCCA GCACTGCCTC 
GTCCACCTTG GAGACATTGC TCGATACAGA AACCAGACTA GCCAGGCAGA GTCCTACTAT 
AGGCATGCAG CTCAGCTTGT CCCCTCCAAT GGTCAGCCTT ACAATCAGTT GGCTATCTTA 
GCTTCTTCCA AAGGAGACCA CCTGACCACA ATTTTCTACT ACTGCAGAAG CATTGCTGTG 
AAATTCCCTT TCCCAGCTGC CTCCACTAAC CTACAAAAAG CACTTTCTAA AGCACTGGAA 
AGCCGGGATG AGTTGAAAAC CAAGTGGGGT GTTTCTGACT TCATCAAGGC CTTTATTAAA 
TTCCACGGTC ATGTATACCT GAGTAAGAGT TTGGAAAAGC TGAGCCCTCT TCGAGAGAAG 
TTAGAAGAAC AGTTTAAGAG ACTGCTGTTC CAAAAAGCTT TCAACTCTCA GCAGTTAGTT 
CATGTCACTG TCATTAACCT GTTTCAACTT CATCATCTTC GTGACTTTAG CAATGAAACA 
GAGCAGCACA GTTACAGCCA AGATGAACAG CTGTGTTGGA CACAGTTGCT GGCCCTCTTC 
ATGTCTTTTC TTGGCATCCT GTGCAAATGT CCTCTCCAGA ATGATTCTCA GGAGTCCTAC 
AATGCCTATC CCCTTCCTGC AGTCAAGGTC TCCATGGACT GGCTAAGACT CCGACCTAGA 
GTCTTTCAAG AAGCTGTAGT GGATGAAAGA CAGTACATCT GGCCCTGGCT AATTTCTCTT 
CTCAATAGTT TTCATCCCCG TGAAGATGAT CTCTCAAGTA CTAATGCCAC ACCACTTCCA 
GAGGAGTTTG AACTACAAGG GTTCCTGGCT TTGAGACCTT CTTTCAGGAA CTTGGATTTT 
TCCAAAGGCC ATCAGGGTAT TACAGGAGAC AAAGAGGGTC AACAACGGCG AATACGGCAG 
CAGCGTTTGA TCTCCATAGG GAAGTGGATT GCTGACAACC AGCCACGGCT GATTCAGTGT 
GAAAATGAGG TAGGGAAATT GTTATTTATC ACAGAAATCC CAGAATTAAT ACTAGAAGAC 
CCCAGTGAAG CCAAAGAGAA CCTCATCCTA CAAGAAACAT CTGCGATAGA GTCACACGCT 
TCGGACGGGA GCCCAGGACT GAAGTCAGTG CTGTCTACAG GCCGAAGTCC AAGCACCAGC 
TGTGACTCAG GAGAGAAGCC AGTGGTCACC TTCAAAGAGA ACATTAAGCC ACGAGAAGTG 
AACAGAGACC AAGGAAGAAG CTTTCCTCCC AAAGAGGTAA AATCCCAGAC AGAACTAAGA 
AAGACCCCAG TGTCCGAAGC CAGGAAAACT CCTGTCACTC AAACTCCAAG TCAAACAAAT 
AATTCTCAGT TCATCCCCAT CCATCACCCT GGAGCCTTCC CTCCTCTTCC CAGCCGACCG 
GGGTTCCCGC CCCCAACATA TGTTATCCCC CCTCCTGTGG CATTTTCTAT GGGCTCAGGA 
TACACCTTCC CAGCTGGTGT TTCTGTCCCA GGAACCTTTC TTCAGTCTAC AGCTCACTCT 
CCAGCAGGAA ACCAGGTGCA AGCTGGGAAA CAGTCCCACA TTCCTTACAG CCAGCAACGG 
CCCTCCGGAC CAGGGCCAAT GAACCAGGGA CCTCAACAAT CACAGCCACC TTCCCAGCCA 
CCCCTTACAT CTTTACCAGC TCAGCCAACA GCACAGTCTA CAAGCCAGTT GCAGGTTCAA 
GCTCTAGCTC AGCAACAGCA GTCCCCTACA AAAGTCATAC CAGCCTTGGG GAAAAGCCCG 
CCTCACCACT CTGGATTCCA GCAGTATCAA CAGGCAGATG CCTCCAAACA GCTGTGGAAT 
CCCCCTCAGG TTCAAAGCCC ACTAGGGAAA ATTATGCCTG TGAAACAGCC CTACTACCTT 
CAGACCCAAG ACCCTATAAA ACTGTTTGAG CCGTCATTGC AGCCTCCTGT AATACAGCAA 
CAGCCTCTAG AGAAAAAAAT GAAGCCTTTC CCCATGGAGC CATATAACCA TAATCCCTCA 
GAAGTCAAGG TCCCAGAGTT CTACTGGGAT TCTTCCTACA GCATGGCTGA TAACAGAGCA 
GTAATGGCTC AGCAACCAAA TATGGACCGC AGGAGCAAAC GGTCACCCGG AGTCTTCCGT 
CCAGAGCAGG ATCCTGTGCC CAGGATGCCG TTTGAGAAAT CCTTACTGGA GAAGCCTTCC 
GAGCTCATGT CACATTCATC TTCTTTCCTG TCCCTCACTG GGTTCTCTGT CAATCAGGAA 
AGATATCCAA ACAGCAGTGT GTTCAATGAA GTGTATGGGA AGAACCTGAC AGCCAGCTCC 
AAGGCAGAGC TGAGCCCCTC GGTTGCCTCC TCCCAGGAAA CATCACTGTA CTCCCTCTTC 
GAAGGGACCC CTTGGTCTCC ATCACTTCCT GCCAGTTCAG ATCATTCAAC ACCAGCCAGC 
CAGTCTCCTC ATTCCTCCAA CCCAAGCAGC CTGCCCAGTT CTCCTCCAAC ACATAACCAT 
AATTCTGCTC CATTCTCTAA TTTTGGACCC ATTGGGACTC CAGATAACAG GGATAGGCGG 
CCTGCAGATA GGTGGAAAAC TGATAAACCA GCCATGGGTG GTTTCGGCGT TGATTACCTC 
TCAGCAACGT CATCATCTGA GAACAGTTGG CATCAGGCTA GCACTCCAAG TGGCACCTGG 
ACAGGCCACG GCCCCTCCAT GGAGGATTCC TCCGCTGTCC TCATGGAAAG CCTAAAGTCT 
ATCTGGTCCA GTTCCATGAT GCATCCTGGA CCCTCCGCTC TCGAGCAGTT GTTAATGCAG 
CAGAAGCAGA AACAGCAGCG GGGACAAGGT GCCATGAACC CTCCACACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Telomeric repeat containing RNA and RNA surveillance factors at mammalian chromosome ends.
Science (New York, N.Y.)318(5851)798-801(2007 Nov)
Azzalin CM,Reichenbach P,Khoriauli L,Giulotto E,Lingner J

Phosphorylation of hUPF1 induces formation of mRNA surveillance complexes containing hSMG-5 and hSMG-7.
Molecular cell12(5)1187-200(2003 Nov)
Ohnishi T,Yamashita A,Kashima I,Schell T,Anders KR,Grimson A,Hachiya T,Hentze MW,Anderson P,Ohno S

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Smg7 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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