smg7 cDNA ORF clone, Danio rerio(zebrafish)

The following smg7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the smg7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa03516 NM_001045083.2
Latest version!
Danio rerio SMG7 nonsense mediated mRNA decay factor (smg7), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
ODa07221 XM_005171308.4
Latest version!
Danio rerio SMG7 nonsense mediated mRNA decay factor (smg7), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
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ODa07221 XM_005171308.3 Danio rerio SMG7 nonsense mediated mRNA decay factor (smg7), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa03516
    Clone ID Related Accession (Same CDS sequence) NM_001045083.2
    Accession Version NM_001045083.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3393bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-11-23
    Organism Danio rerio(zebrafish)
    Product protein SMG7
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC162970.1. On Jun 22, 2008 this sequence version replaced NM_001045083.1. ##Evidence-Data-START## Transcript exon combination :: BC162970.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAACCTCT GCGCCCAATA CTTACGGCAG GCAGAGGCCC TGAAGGCTGA CATGACAGAT 
    TCTAAGCTTG GTGCCGCGGA GGTCTGGACG TCACGACAGG CTCTTCAGGA CTTGTACCAG
    AAAATGCTGG TTACGGACCT GGAGTACGCC CTGGACAAGA AAGTCGAGCA AGATCTCTGG
    AACCATGCTT TTAAGAATCA AATAACGACA TTACAGAGTC AAGCTAAGAA CAGGGCCAAT
    CCCAATCGAA GTGAAGTACA GGCCAATCTC TCCCTGTTTC TTGAGGCAGC TAGTGGATTC
    TACACACAAC TGTTGCAGGA GCTGTGCACA GTGTTTAATG TGGACTTGCC TTGCCGTGTG
    AAGTCATCTC AGCTTGGCAT CATCAGCAAT AAACAGAGCA GCACCAGCGC CATCGTGAAG
    CCCCAGCCCA GCTCCTGTTC CTACATCTGC CAGCATTGTC TCGTCCATCT CGGAGATATT
    GCACGTTATC GGAACCAAAC CAGCCAGGCT GAGTCATACT ACAGACATGC AGCTCAACTT
    GTTCCCTCAA ATGGTCAACC TTACAATCAG CTGGCCATAC TGGCCTCCTC CAAAGGAGAC
    CACCTCACAA CAATCTTCTA CTACTGCAGG AGCATTGCTG TCAAATTCCC CTTTCCTGCT
    GCCTCCACCA ACCTACAAAA AGCTCTTTCC AAGGCCCTTG AGAGCCGTGA TGAGGTCAAA
    ACAAAATGGA GTGTGTCGGA TTTCATTAAA GCCTTCATCA AGTTCCACGG CCATGTTTAT
    CTCTGCAAAA GCCTGGATAA GCTGAACACC CTCAGAGAGA AACTAGAAGA ACAGTTCCAG
    AGGCTGATTC TCCAGAAAGC CTTCAGCTCC CAGCAGCTGG TCCACATCAC AGTGATCAAC
    CTGTTTGAAC TGCATCACCT CCGAGACTTG AGCAATGAAG CAGATGAGCA CAGCTACAGC
    TCGGATGAGC AGATCAGCTG GATTCAACTC CTCGGCCTGT TCATGTCTTT CCTGGGAGTG
    ATGCTAAGTC GAGCTTTACT GAATAAGAAC CGAGAGGAGA TTATGGGAGA ATGTCCACTG
    CCTGCCATTA AAGTATCTCT GGACTGGCTG AGGCTTCGAC CCACTGTGTT TAATGAGAGT
    GCCATGGACA AGCGACAGTA CATCTGGCCA TGGCTGGTGT CCATTCTGAA CAGCTTTCAG
    CCTAAAGAGG AGGATGTGTC AAATGCATCA GTGATTCCAC TGCCTGAGGA GTTTGAATTG
    CAAGGCTTTC TTGCTCTTCG GCCTGCTTTG AGGATGCTAG ACTTCTCGAA AGGGCATCAG
    GGTATAGTGA TTGGCAAAGA GAGTCTGTTA ATTCATGCCA GACACCAGAG ACTCATCAGC
    TTGGGCAAGT GGGTGGCTGA TAACCAACCT CGGTTGATCC AGTGTCGCAT AAGTGATGGC
    CTTGTGCTCT TCATCACTGA CATCCCTGAG ATAGTGGTGG AAGAGCCACA GGAAAAAGAC
    ACACCGGTCC TTCAGGAGTC CTCTAATGGC GAACAGACCC CTAACGAAAG CACCCATGGC
    CTGAAGTCAG TCCTCTCAGC CGGAAAGAAC CAGAACAGTG GGCTGGATGG CAGCGAGAGG
    CCCGTGGTCA CATTCAAAGA GAACATCAAA CCTCGAGAGC AGAGCAGAGA GCAGAACCGG
    AACCAGAACC AGAGGGACAC TGGGAAGGAC AGAGCAGGAT TTAATAAAGG AAATGGGGTA
    CAAGGGAAGA ATGAGCAGAA GAAAGAGGGC AAGAGGAAGA GCGAGGTGAA GAAAAACAGT
    CATGATAAAA CAACAGATGC TGGGAAACAG GTGAAGACTC AGACTGAGTT GAGAAAGACT
    CCAGTGTCTG AGGCGAGGAA GACTCCGGTC ACACAGACTC AAACCACATG CTCTTCACAG
    TTTATACCCA TCCACCACCC AGGAGCTTTC CCTCCTCTGC CGAGCAGACC AGGTTTTCCC
    CCACCAGCAT ATGTGATCCC TCCTCCTGTG GCGTTCTCCA TGAGCCCAGG CTTCACCTTC
    CCTACGGGTG TGTCTGTACC TGCACCCTTT CTTCAGACCG CTTCTCATCC TCAGTCTGCC
    AATCCTGTCC AGACTGGAAA ACCCTCACAC ATTCCCTACA GCCAGCAGAG GCCCTCGGGC
    CCTGTAGCCC TAAACCAGGG CCCTCCCCAG CCTCAGCAGA CCCAGCCTCC CCCTCCACAA
    ACATCCCAAC AGGCTCTGCA ACAATCTGTG CAGCTCCAAC TACAGCAGCA GCAGCAACAA
    CAACAGCAGC AGCAGCAGCA GCAGCAGCAG TCACCCACCA AGCAGAGCTC TCAGCTTGGG
    AAAAGCCCTC CTCATCATCA CAGCATGCAG CAGTCTTACA TGCAGGTGCC TGAGCAGCCT
    GGCCAAATGT GGAACCAGCA CCAAAATCAG CCCGGCATGC AAAAAATGCC CATGCAGATG
    CCAGTCAAAC AGCCTTTCTT CATGCCCACC CAGGACCCAA TGAAGCTCTT TGAACACCCT
    ATGAGCATGC AGCCCCAACA GCCCAGCATG GATAAGAAGA TGAAGTTTCC AGAGGTCAAA
    GTGCAGGATT TCTACTGGGA CCCCCCTTAC CACATGGCAG GAGAGGGCAG GAGCGCAATG
    GCGGACCGAA TGGGCAAACG TCAGCCTGGA GTCTTTTGCT CTGACCAGGA GAACATGCCC
    AGAGGCCCTC CATATGAATT GCCAAATCAG TCTCGGCTTG ACAGTGGAGC TGATGTGATT
    GGTCAGTCCT CCCTTCTTCC TCGATTTTCC ATTCAGGATA ACTCCTACCA GAACAACAGC
    ATTTTTAGTG AGGCCTACGG TAAAAACATG ACACCTTCAT CTAAGCCGGA TGCACCCATG
    ATGCATCAGG AGCCCTCACT CTATTCCCTG TTTGAAGGAA ATCCCTGGTC TCCCTCCCTT
    CCTGCCAGCT CAGATCACTC AACCCCAGCA AGCCAGTCCC CTCACTCCTC CAATCCCAGC
    AGTCTGCCTT CATCCCCACC CACCCACAGC CATGGCTCCA TGCCCTTCTC CAACTTTGGC
    CCCATCGGGA CGCCAGACAG CAGAGACCGC CGAGCAAATG ATCGCTGGAA GGCTGAGAAG
    ACTGGTGTGA GTGGCTTTGG GCTGGATTAC CTGCCTTCTG CCTCGACCTC GTCTGTTCCA
    GAGACCAACA GCTGGCACCA GGGGGCGCCA ACCAGCACCT GGGCAGCCCA GGACATGCCA
    ATGGAGGACT CCTCTACTGT CCTACTTGAC AGTTTCAAGT CAATCTGGTC GAGCTCCATG
    ATGCAGCCTG GTCCGTCTGC GCTGGAGCAG CTCCTGATGC AGCAGAAACA GAAGCAACAG
    CGCGGACACG GCAACATGAA CCCGCCACAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001038548.2
    CDS69..3461
    Translation

    Target ORF information:

    RefSeq Version NM_001045083.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio SMG7 nonsense mediated mRNA decay factor (smg7), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001045083.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAACCTCT GCGCCCAATA CTTACGGCAG GCAGAGGCCC TGAAGGCTGA CATGACAGAT 
    TCTAAGCTTG GTGCCGCGGA GGTCTGGACG TCACGACAGG CTCTTCAGGA CTTGTACCAG
    AAAATGCTGG TTACGGACCT GGAGTACGCC CTGGACAAGA AAGTCGAGCA AGATCTCTGG
    AACCATGCTT TTAAGAATCA AATAACGACA TTACAGAGTC AAGCTAAGAA CAGGGCCAAT
    CCCAATCGAA GTGAAGTACA GGCCAATCTC TCCCTGTTTC TTGAGGCAGC TAGTGGATTC
    TACACACAAC TGTTGCAGGA GCTGTGCACA GTGTTTAATG TGGACTTGCC TTGCCGTGTG
    AAGTCATCTC AGCTTGGCAT CATCAGCAAT AAACAGAGCA GCACCAGCGC CATCGTGAAG
    CCCCAGCCCA GCTCCTGTTC CTACATCTGC CAGCATTGTC TCGTCCATCT CGGAGATATT
    GCACGTTATC GGAACCAAAC CAGCCAGGCT GAGTCATACT ACAGACATGC AGCTCAACTT
    GTTCCCTCAA ATGGTCAACC TTACAATCAG CTGGCCATAC TGGCCTCCTC CAAAGGAGAC
    CACCTCACAA CAATCTTCTA CTACTGCAGG AGCATTGCTG TCAAATTCCC CTTTCCTGCT
    GCCTCCACCA ACCTACAAAA AGCTCTTTCC AAGGCCCTTG AGAGCCGTGA TGAGGTCAAA
    ACAAAATGGA GTGTGTCGGA TTTCATTAAA GCCTTCATCA AGTTCCACGG CCATGTTTAT
    CTCTGCAAAA GCCTGGATAA GCTGAACACC CTCAGAGAGA AACTAGAAGA ACAGTTCCAG
    AGGCTGATTC TCCAGAAAGC CTTCAGCTCC CAGCAGCTGG TCCACATCAC AGTGATCAAC
    CTGTTTGAAC TGCATCACCT CCGAGACTTG AGCAATGAAG CAGATGAGCA CAGCTACAGC
    TCGGATGAGC AGATCAGCTG GATTCAACTC CTCGGCCTGT TCATGTCTTT CCTGGGAGTG
    ATGCTAAGTC GAGCTTTACT GAATAAGAAC CGAGAGGAGA TTATGGGAGA ATGTCCACTG
    CCTGCCATTA AAGTATCTCT GGACTGGCTG AGGCTTCGAC CCACTGTGTT TAATGAGAGT
    GCCATGGACA AGCGACAGTA CATCTGGCCA TGGCTGGTGT CCATTCTGAA CAGCTTTCAG
    CCTAAAGAGG AGGATGTGTC AAATGCATCA GTGATTCCAC TGCCTGAGGA GTTTGAATTG
    CAAGGCTTTC TTGCTCTTCG GCCTGCTTTG AGGATGCTAG ACTTCTCGAA AGGGCATCAG
    GGTATAGTGA TTGGCAAAGA GAGTCTGTTA ATTCATGCCA GACACCAGAG ACTCATCAGC
    TTGGGCAAGT GGGTGGCTGA TAACCAACCT CGGTTGATCC AGTGTCGCAT AAGTGATGGC
    CTTGTGCTCT TCATCACTGA CATCCCTGAG ATAGTGGTGG AAGAGCCACA GGAAAAAGAC
    ACACCGGTCC TTCAGGAGTC CTCTAATGGC GAACAGACCC CTAACGAAAG CACCCATGGC
    CTGAAGTCAG TCCTCTCAGC CGGAAAGAAC CAGAACAGTG GGCTGGATGG CAGCGAGAGG
    CCCGTGGTCA CATTCAAAGA GAACATCAAA CCTCGAGAGC AGAGCAGAGA GCAGAACCGG
    AACCAGAACC AGAGGGACAC TGGGAAGGAC AGAGCAGGAT TTAATAAAGG AAATGGGGTA
    CAAGGGAAGA ATGAGCAGAA GAAAGAGGGC AAGAGGAAGA GCGAGGTGAA GAAAAACAGT
    CATGATAAAA CAACAGATGC TGGGAAACAG GTGAAGACTC AGACTGAGTT GAGAAAGACT
    CCAGTGTCTG AGGCGAGGAA GACTCCGGTC ACACAGACTC AAACCACATG CTCTTCACAG
    TTTATACCCA TCCACCACCC AGGAGCTTTC CCTCCTCTGC CGAGCAGACC AGGTTTTCCC
    CCACCAGCAT ATGTGATCCC TCCTCCTGTG GCGTTCTCCA TGAGCCCAGG CTTCACCTTC
    CCTACGGGTG TGTCTGTACC TGCACCCTTT CTTCAGACCG CTTCTCATCC TCAGTCTGCC
    AATCCTGTCC AGACTGGAAA ACCCTCACAC ATTCCCTACA GCCAGCAGAG GCCCTCGGGC
    CCTGTAGCCC TAAACCAGGG CCCTCCCCAG CCTCAGCAGA CCCAGCCTCC CCCTCCACAA
    ACATCCCAAC AGGCTCTGCA ACAATCTGTG CAGCTCCAAC TACAGCAGCA GCAGCAACAA
    CAACAGCAGC AGCAGCAGCA GCAGCAGCAG TCACCCACCA AGCAGAGCTC TCAGCTTGGG
    AAAAGCCCTC CTCATCATCA CAGCATGCAG CAGTCTTACA TGCAGGTGCC TGAGCAGCCT
    GGCCAAATGT GGAACCAGCA CCAAAATCAG CCCGGCATGC AAAAAATGCC CATGCAGATG
    CCAGTCAAAC AGCCTTTCTT CATGCCCACC CAGGACCCAA TGAAGCTCTT TGAACACCCT
    ATGAGCATGC AGCCCCAACA GCCCAGCATG GATAAGAAGA TGAAGTTTCC AGAGGTCAAA
    GTGCAGGATT TCTACTGGGA CCCCCCTTAC CACATGGCAG GAGAGGGCAG GAGCGCAATG
    GCGGACCGAA TGGGCAAACG TCAGCCTGGA GTCTTTTGCT CTGACCAGGA GAACATGCCC
    AGAGGCCCTC CATATGAATT GCCAAATCAG TCTCGGCTTG ACAGTGGAGC TGATGTGATT
    GGTCAGTCCT CCCTTCTTCC TCGATTTTCC ATTCAGGATA ACTCCTACCA GAACAACAGC
    ATTTTTAGTG AGGCCTACGG TAAAAACATG ACACCTTCAT CTAAGCCGGA TGCACCCATG
    ATGCATCAGG AGCCCTCACT CTATTCCCTG TTTGAAGGAA ATCCCTGGTC TCCCTCCCTT
    CCTGCCAGCT CAGATCACTC AACCCCAGCA AGCCAGTCCC CTCACTCCTC CAATCCCAGC
    AGTCTGCCTT CATCCCCACC CACCCACAGC CATGGCTCCA TGCCCTTCTC CAACTTTGGC
    CCCATCGGGA CGCCAGACAG CAGAGACCGC CGAGCAAATG ATCGCTGGAA GGCTGAGAAG
    ACTGGTGTGA GTGGCTTTGG GCTGGATTAC CTGCCTTCTG CCTCGACCTC GTCTGTTCCA
    GAGACCAACA GCTGGCACCA GGGGGCGCCA ACCAGCACCT GGGCAGCCCA GGACATGCCA
    ATGGAGGACT CCTCTACTGT CCTACTTGAC AGTTTCAAGT CAATCTGGTC GAGCTCCATG
    ATGCAGCCTG GTCCGTCTGC GCTGGAGCAG CTCCTGATGC AGCAGAAACA GAAGCAACAG
    CGCGGACACG GCAACATGAA CCCGCCACAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa07221
    Clone ID Related Accession (Same CDS sequence) XM_005171308.3 , XM_005171308.4
    Accession Version XM_005171308.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3543bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product protein SMG7 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005171308.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAACCTCT GCGCCCAATA CTTACGGCAG GCAGAGGCCC TGAAGGCTGA CATGACAGAT 
    TCTAAGCTTG GTGCCGCGGA GGTCTGGACG TCACGACAGG CTCTTCAGGA CTTGTACCAG
    AAAATGCTGG TTACGGACCT GGAGTACGCC CTGGACAAGA AAGTTGAGCA AGATCTCTGG
    AACCATGCTT TTAAGAATCA AATAACGACA TTACAGAGTC AAGCTAAGAA CAGGGCCAAT
    CCCAATCGAA GTGAAGTACA GGCCAATCTC TCCCTGTTTC TTGAGGCAGC TAGTGGATTC
    TACACACAAC TGTTGCAGGA GCTGTGCACA GTGTTTAATG TGGACTTGCC TTGCCGTGTG
    AAGTCATCTC AGCTTGGCAT CATCAGCAAT AAACAGAGCA GCACCAGCGC CATCGTGAAG
    CCCCAGCCCA GCTCCTGTTC CTACATCTGC CAGCATTGTC TCGTCCATCT CGGAGATATT
    GCACGTTATC GGAACCAAAC CAGCCAGGCT GAGTCATACT ACAGACATGC AGCTCAACTT
    GTTCCCTCAA ATGGTCAACC TTACAATCAG CTGGCCATAC TGGCCTCCTC CAAAGGAGAC
    CACCTCACAA CAATCTTCTA CTACTGCAGG AGCATTGCTG TCAAATTCCC CTTTCCTGCT
    GCCTCCACCA ACCTACAAAA AGCTCTTTCC AAGGCCCTTG AGAGCCGTGA TGAGGTCAAA
    ACAAAATGGA GTGTGTCGGA TTTCATTAAA GCCTTCATCA AGTTCCACGG CCATGTTTAT
    CTCTGCAAAA GCCTGGATAA GCTGAACACC CTCAGAGAGA AACTAGAAGA ACAGTTCCAG
    AGGCTGATTC TCCAGAAAGC CTTCAGCTCC CAGCAGCTGG TCCACATCAC GGTGATCAAC
    CTGTTTGAAC TGCATCACCT CCGAGACTTG AGCAATGAAG CAGATGAGCA CAGCTACAGC
    TCGGATGAGC AGATCAGCTG GATTCAACTC CTCGGCCTGT TCATGTCTTT TCTGGGAGTG
    ATGCTAAGTC GAGCTTTACT GAATAAGAAC CGAGAGGAGA TTATGGGAGA ATGTCCACTG
    CCTGCCATTA AAGTATCTCT GGACTGGCTG AGGCTTCGAC CCACTGTGTT TAATGAGAGT
    GCCATGGACA AGCGACAGTA CATCTGGCCA TGGCTGGTGT CCATTCTGAA CAGCTTTCAG
    CCTAAAGAGG AGGATGTGTC AAATGCATCA GTGATTCCAC TGCCTGAGGA GTTTGAATTG
    CAAGGCTTTC TTGCTCTTCG GCCTGCTTTG AGGATGCTAG ACTTCTCGAA AGGGCATCAG
    GGTATAGTGA TTGGCAAAGA GAGTCTGTTA ATTCATGCCA GACACCAGAG ACTCATCAGC
    TTGGGCAAGT GGGTGGCTGA TAACCAACCT CGGTTGATCC AGTGTCGCAT GAGTGATGGC
    CTTGTGCTCT TCATCACTGA CATCCCTGAG ATAGTGGTGG AAGAGCCACA GGAAAAAGAC
    ACACCGGTCC TTCAGGAGTC CTCTAATGGC GAACAGACCC CTAACGAAAG CACCCATGGC
    CTGAAGTCAG TCCTCTCAGC CGGAAAGAAC CAGAACAGTG GGCTGGATGG CAGCGAGAGG
    CCCGTGGTCA CATTCAAAGA GAACATCAAA CCTCGAGAGC AGAGCAGAGA GCAGAACCGG
    AACCAGAACC AGAGGGACAC TGGGAAGGAC AGAGCAGGAT TTAATAAAGG AAATGGGGTA
    CAAGGGAAGA ATGAGCAGAA GAAAGAGGGC AAGAGGAAGA GCGAGGTGAA GAAAAACAGT
    CATGATAAAA CAACAGATGC TGGGAAACAG GTGAAGACTC AGACTGAGTT GAGAAAGACT
    CCAGTGTCTG AGGCGAGGAA GACTCCGGTC ACACAGACTC AAACCACATG CTCTTCACAG
    TTTATACCCA TCCACCACCC AGGAGCTTTC CCTCCTCTGC CGAGCAGACC AGGTTTTCCC
    CCACCAGCAT ATGTGATCCC TCCTCCTGTG GCGTTCTCCA TGAGCCCAGG CTTCACCTTC
    CCTACGGGTG TATCTGTACC TGCACCCTTT CTTCAGACCG CTTCTCATCC TCAGTCTGCC
    AATCCTGTCC AGACTGGAAA ACCCTCACAC ATTCCCTACA GCCAGCAGAG GCCCTCAGGC
    CCTGTAGCCC TAAACCAGGG CCCTCCCCAG CCTCAGCAGA CCCAGCCTCC CCCTCCACAA
    ACATCCCAAC AGGCTCTGCA ACAATCTGTG CAGCTCCAAC TACAGCAGCA GCAGCAACAA
    CAACAGCAGC AGCAGCAGCA GCAGCAGCAG TCACCCACCA AGCAGAGCTC TCAGCTCGGG
    AAAAGCCCTC CTCATCATCA CAGCATGCAG CAGTCTTACA TGCAGGTGCC TGAGCAGCCT
    GGCCAAATGT GGAACCAGCA CCAAAATCAG CCCGGCATGC AGAAAATGCC CATGCAGATG
    CCAGTCAAAC AGCCTTTCTT CATGCCCACC CAGGACCCAA TGAAGCTCTT TGAACACCCT
    ATGAGCATGC AGCCCCAACA GCCCAGCATG GATAAGAAGA TGAAGTTTCC AGAGGTCAAA
    GTGCAGGATT TCTACTGGGA CCCCCCTTAC CACATGGCAG GAGAGGGAAG GAGCACAATG
    GCGGACCGAA TGGGCAAACG TCAGCCTGGA GTCTTTTGCT CTGACCAGGA GAACATGCCC
    AGAGGCCCTC CATATGAAGA CAACAAGAGC TCCCCTCTTC TGCCCCCAGA CCTGTTAAAA
    ACTCTGGCGG ATTTTGAGGA GGAGGAGGAG CTGCTCTTTA CTAAACCTCA TGATTTCTAC
    CAGGCCTTGG CTGGTCCTCT TAATTCTGCT CCTGGAAGGA ACATGTTTTT GCCAAATCAG
    TCTCGGCTTG ACAGTGGAGC TGATGTGATT GGTCAGTCCT CCCTTCTTCC TCGATTTTCC
    ATTCAGGATA ACTCCTACCA GAACAACAGC ATTTTTAGTG AGGCCTACGG TAAAAACATG
    ACACCTTCAT CTAAGCCGGA TGCACCCATG ATGCATCAGG AGCCCTCACT CTATTCCCTG
    TTTGAAGGAA ATCCCTGGTC TCCCTCCCTT CCTGCCAGCT CAGATCACTC AACCCCAGCA
    AGCCAGTCCC CTCACTCCTC CAATCCCAGC AGTCTGCCTT CATCCCCACC CACCCACAGC
    CATGGCTCCA TGCCCTTCTC CAACTTTGGC CCCATCGGGA CGCCAGACAG CAGAGACCGC
    CGAGCAAATG ATCGCTGGAA GGCTGAGAAG ACTGGTGTGA GTGGCTTTGG GCTGGATTAC
    CTGCCTTCTG CCTCGACCTC GTCTGTTCCA GAGACCAACA GCTGGCACCA GGGGGCGCCA
    ACCAGCACCT GGGCAGCCCA GGACATGCCA ATGGAGGACT CCTCTACTGT CCTACTTGAC
    AGTTTCAAGT CAATCTGGTC GAGCTCCATG ATGCAGCCTG GTCCGTCTGC GCTGGAGCAG
    CTCCTGATGC AGCAGAAACA GAAGCAACAG CGCGGACACG GCAACATGAA CCCGCCACAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005171365.1
    CDS98..3640
    Translation

    Target ORF information:

    RefSeq Version XM_005171308.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio SMG7 nonsense mediated mRNA decay factor (smg7), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005171308.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAACCTCT GCGCCCAATA CTTACGGCAG GCAGAGGCCC TGAAGGCTGA CATGACAGAT 
    TCTAAGCTTG GTGCCGCGGA GGTCTGGACG TCACGACAGG CTCTTCAGGA CTTGTACCAG
    AAAATGCTGG TTACGGACCT GGAGTACGCC CTGGACAAGA AAGTTGAGCA AGATCTCTGG
    AACCATGCTT TTAAGAATCA AATAACGACA TTACAGAGTC AAGCTAAGAA CAGGGCCAAT
    CCCAATCGAA GTGAAGTACA GGCCAATCTC TCCCTGTTTC TTGAGGCAGC TAGTGGATTC
    TACACACAAC TGTTGCAGGA GCTGTGCACA GTGTTTAATG TGGACTTGCC TTGCCGTGTG
    AAGTCATCTC AGCTTGGCAT CATCAGCAAT AAACAGAGCA GCACCAGCGC CATCGTGAAG
    CCCCAGCCCA GCTCCTGTTC CTACATCTGC CAGCATTGTC TCGTCCATCT CGGAGATATT
    GCACGTTATC GGAACCAAAC CAGCCAGGCT GAGTCATACT ACAGACATGC AGCTCAACTT
    GTTCCCTCAA ATGGTCAACC TTACAATCAG CTGGCCATAC TGGCCTCCTC CAAAGGAGAC
    CACCTCACAA CAATCTTCTA CTACTGCAGG AGCATTGCTG TCAAATTCCC CTTTCCTGCT
    GCCTCCACCA ACCTACAAAA AGCTCTTTCC AAGGCCCTTG AGAGCCGTGA TGAGGTCAAA
    ACAAAATGGA GTGTGTCGGA TTTCATTAAA GCCTTCATCA AGTTCCACGG CCATGTTTAT
    CTCTGCAAAA GCCTGGATAA GCTGAACACC CTCAGAGAGA AACTAGAAGA ACAGTTCCAG
    AGGCTGATTC TCCAGAAAGC CTTCAGCTCC CAGCAGCTGG TCCACATCAC GGTGATCAAC
    CTGTTTGAAC TGCATCACCT CCGAGACTTG AGCAATGAAG CAGATGAGCA CAGCTACAGC
    TCGGATGAGC AGATCAGCTG GATTCAACTC CTCGGCCTGT TCATGTCTTT TCTGGGAGTG
    ATGCTAAGTC GAGCTTTACT GAATAAGAAC CGAGAGGAGA TTATGGGAGA ATGTCCACTG
    CCTGCCATTA AAGTATCTCT GGACTGGCTG AGGCTTCGAC CCACTGTGTT TAATGAGAGT
    GCCATGGACA AGCGACAGTA CATCTGGCCA TGGCTGGTGT CCATTCTGAA CAGCTTTCAG
    CCTAAAGAGG AGGATGTGTC AAATGCATCA GTGATTCCAC TGCCTGAGGA GTTTGAATTG
    CAAGGCTTTC TTGCTCTTCG GCCTGCTTTG AGGATGCTAG ACTTCTCGAA AGGGCATCAG
    GGTATAGTGA TTGGCAAAGA GAGTCTGTTA ATTCATGCCA GACACCAGAG ACTCATCAGC
    TTGGGCAAGT GGGTGGCTGA TAACCAACCT CGGTTGATCC AGTGTCGCAT GAGTGATGGC
    CTTGTGCTCT TCATCACTGA CATCCCTGAG ATAGTGGTGG AAGAGCCACA GGAAAAAGAC
    ACACCGGTCC TTCAGGAGTC CTCTAATGGC GAACAGACCC CTAACGAAAG CACCCATGGC
    CTGAAGTCAG TCCTCTCAGC CGGAAAGAAC CAGAACAGTG GGCTGGATGG CAGCGAGAGG
    CCCGTGGTCA CATTCAAAGA GAACATCAAA CCTCGAGAGC AGAGCAGAGA GCAGAACCGG
    AACCAGAACC AGAGGGACAC TGGGAAGGAC AGAGCAGGAT TTAATAAAGG AAATGGGGTA
    CAAGGGAAGA ATGAGCAGAA GAAAGAGGGC AAGAGGAAGA GCGAGGTGAA GAAAAACAGT
    CATGATAAAA CAACAGATGC TGGGAAACAG GTGAAGACTC AGACTGAGTT GAGAAAGACT
    CCAGTGTCTG AGGCGAGGAA GACTCCGGTC ACACAGACTC AAACCACATG CTCTTCACAG
    TTTATACCCA TCCACCACCC AGGAGCTTTC CCTCCTCTGC CGAGCAGACC AGGTTTTCCC
    CCACCAGCAT ATGTGATCCC TCCTCCTGTG GCGTTCTCCA TGAGCCCAGG CTTCACCTTC
    CCTACGGGTG TATCTGTACC TGCACCCTTT CTTCAGACCG CTTCTCATCC TCAGTCTGCC
    AATCCTGTCC AGACTGGAAA ACCCTCACAC ATTCCCTACA GCCAGCAGAG GCCCTCAGGC
    CCTGTAGCCC TAAACCAGGG CCCTCCCCAG CCTCAGCAGA CCCAGCCTCC CCCTCCACAA
    ACATCCCAAC AGGCTCTGCA ACAATCTGTG CAGCTCCAAC TACAGCAGCA GCAGCAACAA
    CAACAGCAGC AGCAGCAGCA GCAGCAGCAG TCACCCACCA AGCAGAGCTC TCAGCTCGGG
    AAAAGCCCTC CTCATCATCA CAGCATGCAG CAGTCTTACA TGCAGGTGCC TGAGCAGCCT
    GGCCAAATGT GGAACCAGCA CCAAAATCAG CCCGGCATGC AGAAAATGCC CATGCAGATG
    CCAGTCAAAC AGCCTTTCTT CATGCCCACC CAGGACCCAA TGAAGCTCTT TGAACACCCT
    ATGAGCATGC AGCCCCAACA GCCCAGCATG GATAAGAAGA TGAAGTTTCC AGAGGTCAAA
    GTGCAGGATT TCTACTGGGA CCCCCCTTAC CACATGGCAG GAGAGGGAAG GAGCACAATG
    GCGGACCGAA TGGGCAAACG TCAGCCTGGA GTCTTTTGCT CTGACCAGGA GAACATGCCC
    AGAGGCCCTC CATATGAAGA CAACAAGAGC TCCCCTCTTC TGCCCCCAGA CCTGTTAAAA
    ACTCTGGCGG ATTTTGAGGA GGAGGAGGAG CTGCTCTTTA CTAAACCTCA TGATTTCTAC
    CAGGCCTTGG CTGGTCCTCT TAATTCTGCT CCTGGAAGGA ACATGTTTTT GCCAAATCAG
    TCTCGGCTTG ACAGTGGAGC TGATGTGATT GGTCAGTCCT CCCTTCTTCC TCGATTTTCC
    ATTCAGGATA ACTCCTACCA GAACAACAGC ATTTTTAGTG AGGCCTACGG TAAAAACATG
    ACACCTTCAT CTAAGCCGGA TGCACCCATG ATGCATCAGG AGCCCTCACT CTATTCCCTG
    TTTGAAGGAA ATCCCTGGTC TCCCTCCCTT CCTGCCAGCT CAGATCACTC AACCCCAGCA
    AGCCAGTCCC CTCACTCCTC CAATCCCAGC AGTCTGCCTT CATCCCCACC CACCCACAGC
    CATGGCTCCA TGCCCTTCTC CAACTTTGGC CCCATCGGGA CGCCAGACAG CAGAGACCGC
    CGAGCAAATG ATCGCTGGAA GGCTGAGAAG ACTGGTGTGA GTGGCTTTGG GCTGGATTAC
    CTGCCTTCTG CCTCGACCTC GTCTGTTCCA GAGACCAACA GCTGGCACCA GGGGGCGCCA
    ACCAGCACCT GGGCAGCCCA GGACATGCCA ATGGAGGACT CCTCTACTGT CCTACTTGAC
    AGTTTCAAGT CAATCTGGTC GAGCTCCATG ATGCAGCCTG GTCCGTCTGC GCTGGAGCAG
    CTCCTGATGC AGCAGAAACA GAAGCAACAG CGCGGACACG GCAACATGAA CCCGCCACAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa07221
    Clone ID Related Accession (Same CDS sequence) XM_005171308.3 , XM_005171308.4
    Accession Version XM_005171308.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3543bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product protein SMG7 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005171308.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAACCTCT GCGCCCAATA CTTACGGCAG GCAGAGGCCC TGAAGGCTGA CATGACAGAT 
    TCTAAGCTTG GTGCCGCGGA GGTCTGGACG TCACGACAGG CTCTTCAGGA CTTGTACCAG
    AAAATGCTGG TTACGGACCT GGAGTACGCC CTGGACAAGA AAGTTGAGCA AGATCTCTGG
    AACCATGCTT TTAAGAATCA AATAACGACA TTACAGAGTC AAGCTAAGAA CAGGGCCAAT
    CCCAATCGAA GTGAAGTACA GGCCAATCTC TCCCTGTTTC TTGAGGCAGC TAGTGGATTC
    TACACACAAC TGTTGCAGGA GCTGTGCACA GTGTTTAATG TGGACTTGCC TTGCCGTGTG
    AAGTCATCTC AGCTTGGCAT CATCAGCAAT AAACAGAGCA GCACCAGCGC CATCGTGAAG
    CCCCAGCCCA GCTCCTGTTC CTACATCTGC CAGCATTGTC TCGTCCATCT CGGAGATATT
    GCACGTTATC GGAACCAAAC CAGCCAGGCT GAGTCATACT ACAGACATGC AGCTCAACTT
    GTTCCCTCAA ATGGTCAACC TTACAATCAG CTGGCCATAC TGGCCTCCTC CAAAGGAGAC
    CACCTCACAA CAATCTTCTA CTACTGCAGG AGCATTGCTG TCAAATTCCC CTTTCCTGCT
    GCCTCCACCA ACCTACAAAA AGCTCTTTCC AAGGCCCTTG AGAGCCGTGA TGAGGTCAAA
    ACAAAATGGA GTGTGTCGGA TTTCATTAAA GCCTTCATCA AGTTCCACGG CCATGTTTAT
    CTCTGCAAAA GCCTGGATAA GCTGAACACC CTCAGAGAGA AACTAGAAGA ACAGTTCCAG
    AGGCTGATTC TCCAGAAAGC CTTCAGCTCC CAGCAGCTGG TCCACATCAC GGTGATCAAC
    CTGTTTGAAC TGCATCACCT CCGAGACTTG AGCAATGAAG CAGATGAGCA CAGCTACAGC
    TCGGATGAGC AGATCAGCTG GATTCAACTC CTCGGCCTGT TCATGTCTTT TCTGGGAGTG
    ATGCTAAGTC GAGCTTTACT GAATAAGAAC CGAGAGGAGA TTATGGGAGA ATGTCCACTG
    CCTGCCATTA AAGTATCTCT GGACTGGCTG AGGCTTCGAC CCACTGTGTT TAATGAGAGT
    GCCATGGACA AGCGACAGTA CATCTGGCCA TGGCTGGTGT CCATTCTGAA CAGCTTTCAG
    CCTAAAGAGG AGGATGTGTC AAATGCATCA GTGATTCCAC TGCCTGAGGA GTTTGAATTG
    CAAGGCTTTC TTGCTCTTCG GCCTGCTTTG AGGATGCTAG ACTTCTCGAA AGGGCATCAG
    GGTATAGTGA TTGGCAAAGA GAGTCTGTTA ATTCATGCCA GACACCAGAG ACTCATCAGC
    TTGGGCAAGT GGGTGGCTGA TAACCAACCT CGGTTGATCC AGTGTCGCAT GAGTGATGGC
    CTTGTGCTCT TCATCACTGA CATCCCTGAG ATAGTGGTGG AAGAGCCACA GGAAAAAGAC
    ACACCGGTCC TTCAGGAGTC CTCTAATGGC GAACAGACCC CTAACGAAAG CACCCATGGC
    CTGAAGTCAG TCCTCTCAGC CGGAAAGAAC CAGAACAGTG GGCTGGATGG CAGCGAGAGG
    CCCGTGGTCA CATTCAAAGA GAACATCAAA CCTCGAGAGC AGAGCAGAGA GCAGAACCGG
    AACCAGAACC AGAGGGACAC TGGGAAGGAC AGAGCAGGAT TTAATAAAGG AAATGGGGTA
    CAAGGGAAGA ATGAGCAGAA GAAAGAGGGC AAGAGGAAGA GCGAGGTGAA GAAAAACAGT
    CATGATAAAA CAACAGATGC TGGGAAACAG GTGAAGACTC AGACTGAGTT GAGAAAGACT
    CCAGTGTCTG AGGCGAGGAA GACTCCGGTC ACACAGACTC AAACCACATG CTCTTCACAG
    TTTATACCCA TCCACCACCC AGGAGCTTTC CCTCCTCTGC CGAGCAGACC AGGTTTTCCC
    CCACCAGCAT ATGTGATCCC TCCTCCTGTG GCGTTCTCCA TGAGCCCAGG CTTCACCTTC
    CCTACGGGTG TATCTGTACC TGCACCCTTT CTTCAGACCG CTTCTCATCC TCAGTCTGCC
    AATCCTGTCC AGACTGGAAA ACCCTCACAC ATTCCCTACA GCCAGCAGAG GCCCTCAGGC
    CCTGTAGCCC TAAACCAGGG CCCTCCCCAG CCTCAGCAGA CCCAGCCTCC CCCTCCACAA
    ACATCCCAAC AGGCTCTGCA ACAATCTGTG CAGCTCCAAC TACAGCAGCA GCAGCAACAA
    CAACAGCAGC AGCAGCAGCA GCAGCAGCAG TCACCCACCA AGCAGAGCTC TCAGCTCGGG
    AAAAGCCCTC CTCATCATCA CAGCATGCAG CAGTCTTACA TGCAGGTGCC TGAGCAGCCT
    GGCCAAATGT GGAACCAGCA CCAAAATCAG CCCGGCATGC AGAAAATGCC CATGCAGATG
    CCAGTCAAAC AGCCTTTCTT CATGCCCACC CAGGACCCAA TGAAGCTCTT TGAACACCCT
    ATGAGCATGC AGCCCCAACA GCCCAGCATG GATAAGAAGA TGAAGTTTCC AGAGGTCAAA
    GTGCAGGATT TCTACTGGGA CCCCCCTTAC CACATGGCAG GAGAGGGAAG GAGCACAATG
    GCGGACCGAA TGGGCAAACG TCAGCCTGGA GTCTTTTGCT CTGACCAGGA GAACATGCCC
    AGAGGCCCTC CATATGAAGA CAACAAGAGC TCCCCTCTTC TGCCCCCAGA CCTGTTAAAA
    ACTCTGGCGG ATTTTGAGGA GGAGGAGGAG CTGCTCTTTA CTAAACCTCA TGATTTCTAC
    CAGGCCTTGG CTGGTCCTCT TAATTCTGCT CCTGGAAGGA ACATGTTTTT GCCAAATCAG
    TCTCGGCTTG ACAGTGGAGC TGATGTGATT GGTCAGTCCT CCCTTCTTCC TCGATTTTCC
    ATTCAGGATA ACTCCTACCA GAACAACAGC ATTTTTAGTG AGGCCTACGG TAAAAACATG
    ACACCTTCAT CTAAGCCGGA TGCACCCATG ATGCATCAGG AGCCCTCACT CTATTCCCTG
    TTTGAAGGAA ATCCCTGGTC TCCCTCCCTT CCTGCCAGCT CAGATCACTC AACCCCAGCA
    AGCCAGTCCC CTCACTCCTC CAATCCCAGC AGTCTGCCTT CATCCCCACC CACCCACAGC
    CATGGCTCCA TGCCCTTCTC CAACTTTGGC CCCATCGGGA CGCCAGACAG CAGAGACCGC
    CGAGCAAATG ATCGCTGGAA GGCTGAGAAG ACTGGTGTGA GTGGCTTTGG GCTGGATTAC
    CTGCCTTCTG CCTCGACCTC GTCTGTTCCA GAGACCAACA GCTGGCACCA GGGGGCGCCA
    ACCAGCACCT GGGCAGCCCA GGACATGCCA ATGGAGGACT CCTCTACTGT CCTACTTGAC
    AGTTTCAAGT CAATCTGGTC GAGCTCCATG ATGCAGCCTG GTCCGTCTGC GCTGGAGCAG
    CTCCTGATGC AGCAGAAACA GAAGCAACAG CGCGGACACG GCAACATGAA CCCGCCACAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005171365.1
    CDS107..3649
    Translation

    Target ORF information:

    RefSeq Version XM_005171308.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio SMG7 nonsense mediated mRNA decay factor (smg7), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005171308.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAACCTCT GCGCCCAATA CTTACGGCAG GCAGAGGCCC TGAAGGCTGA CATGACAGAT 
    TCTAAGCTTG GTGCCGCGGA GGTCTGGACG TCACGACAGG CTCTTCAGGA CTTGTACCAG
    AAAATGCTGG TTACGGACCT GGAGTACGCC CTGGACAAGA AAGTTGAGCA AGATCTCTGG
    AACCATGCTT TTAAGAATCA AATAACGACA TTACAGAGTC AAGCTAAGAA CAGGGCCAAT
    CCCAATCGAA GTGAAGTACA GGCCAATCTC TCCCTGTTTC TTGAGGCAGC TAGTGGATTC
    TACACACAAC TGTTGCAGGA GCTGTGCACA GTGTTTAATG TGGACTTGCC TTGCCGTGTG
    AAGTCATCTC AGCTTGGCAT CATCAGCAAT AAACAGAGCA GCACCAGCGC CATCGTGAAG
    CCCCAGCCCA GCTCCTGTTC CTACATCTGC CAGCATTGTC TCGTCCATCT CGGAGATATT
    GCACGTTATC GGAACCAAAC CAGCCAGGCT GAGTCATACT ACAGACATGC AGCTCAACTT
    GTTCCCTCAA ATGGTCAACC TTACAATCAG CTGGCCATAC TGGCCTCCTC CAAAGGAGAC
    CACCTCACAA CAATCTTCTA CTACTGCAGG AGCATTGCTG TCAAATTCCC CTTTCCTGCT
    GCCTCCACCA ACCTACAAAA AGCTCTTTCC AAGGCCCTTG AGAGCCGTGA TGAGGTCAAA
    ACAAAATGGA GTGTGTCGGA TTTCATTAAA GCCTTCATCA AGTTCCACGG CCATGTTTAT
    CTCTGCAAAA GCCTGGATAA GCTGAACACC CTCAGAGAGA AACTAGAAGA ACAGTTCCAG
    AGGCTGATTC TCCAGAAAGC CTTCAGCTCC CAGCAGCTGG TCCACATCAC GGTGATCAAC
    CTGTTTGAAC TGCATCACCT CCGAGACTTG AGCAATGAAG CAGATGAGCA CAGCTACAGC
    TCGGATGAGC AGATCAGCTG GATTCAACTC CTCGGCCTGT TCATGTCTTT TCTGGGAGTG
    ATGCTAAGTC GAGCTTTACT GAATAAGAAC CGAGAGGAGA TTATGGGAGA ATGTCCACTG
    CCTGCCATTA AAGTATCTCT GGACTGGCTG AGGCTTCGAC CCACTGTGTT TAATGAGAGT
    GCCATGGACA AGCGACAGTA CATCTGGCCA TGGCTGGTGT CCATTCTGAA CAGCTTTCAG
    CCTAAAGAGG AGGATGTGTC AAATGCATCA GTGATTCCAC TGCCTGAGGA GTTTGAATTG
    CAAGGCTTTC TTGCTCTTCG GCCTGCTTTG AGGATGCTAG ACTTCTCGAA AGGGCATCAG
    GGTATAGTGA TTGGCAAAGA GAGTCTGTTA ATTCATGCCA GACACCAGAG ACTCATCAGC
    TTGGGCAAGT GGGTGGCTGA TAACCAACCT CGGTTGATCC AGTGTCGCAT GAGTGATGGC
    CTTGTGCTCT TCATCACTGA CATCCCTGAG ATAGTGGTGG AAGAGCCACA GGAAAAAGAC
    ACACCGGTCC TTCAGGAGTC CTCTAATGGC GAACAGACCC CTAACGAAAG CACCCATGGC
    CTGAAGTCAG TCCTCTCAGC CGGAAAGAAC CAGAACAGTG GGCTGGATGG CAGCGAGAGG
    CCCGTGGTCA CATTCAAAGA GAACATCAAA CCTCGAGAGC AGAGCAGAGA GCAGAACCGG
    AACCAGAACC AGAGGGACAC TGGGAAGGAC AGAGCAGGAT TTAATAAAGG AAATGGGGTA
    CAAGGGAAGA ATGAGCAGAA GAAAGAGGGC AAGAGGAAGA GCGAGGTGAA GAAAAACAGT
    CATGATAAAA CAACAGATGC TGGGAAACAG GTGAAGACTC AGACTGAGTT GAGAAAGACT
    CCAGTGTCTG AGGCGAGGAA GACTCCGGTC ACACAGACTC AAACCACATG CTCTTCACAG
    TTTATACCCA TCCACCACCC AGGAGCTTTC CCTCCTCTGC CGAGCAGACC AGGTTTTCCC
    CCACCAGCAT ATGTGATCCC TCCTCCTGTG GCGTTCTCCA TGAGCCCAGG CTTCACCTTC
    CCTACGGGTG TATCTGTACC TGCACCCTTT CTTCAGACCG CTTCTCATCC TCAGTCTGCC
    AATCCTGTCC AGACTGGAAA ACCCTCACAC ATTCCCTACA GCCAGCAGAG GCCCTCAGGC
    CCTGTAGCCC TAAACCAGGG CCCTCCCCAG CCTCAGCAGA CCCAGCCTCC CCCTCCACAA
    ACATCCCAAC AGGCTCTGCA ACAATCTGTG CAGCTCCAAC TACAGCAGCA GCAGCAACAA
    CAACAGCAGC AGCAGCAGCA GCAGCAGCAG TCACCCACCA AGCAGAGCTC TCAGCTCGGG
    AAAAGCCCTC CTCATCATCA CAGCATGCAG CAGTCTTACA TGCAGGTGCC TGAGCAGCCT
    GGCCAAATGT GGAACCAGCA CCAAAATCAG CCCGGCATGC AGAAAATGCC CATGCAGATG
    CCAGTCAAAC AGCCTTTCTT CATGCCCACC CAGGACCCAA TGAAGCTCTT TGAACACCCT
    ATGAGCATGC AGCCCCAACA GCCCAGCATG GATAAGAAGA TGAAGTTTCC AGAGGTCAAA
    GTGCAGGATT TCTACTGGGA CCCCCCTTAC CACATGGCAG GAGAGGGAAG GAGCACAATG
    GCGGACCGAA TGGGCAAACG TCAGCCTGGA GTCTTTTGCT CTGACCAGGA GAACATGCCC
    AGAGGCCCTC CATATGAAGA CAACAAGAGC TCCCCTCTTC TGCCCCCAGA CCTGTTAAAA
    ACTCTGGCGG ATTTTGAGGA GGAGGAGGAG CTGCTCTTTA CTAAACCTCA TGATTTCTAC
    CAGGCCTTGG CTGGTCCTCT TAATTCTGCT CCTGGAAGGA ACATGTTTTT GCCAAATCAG
    TCTCGGCTTG ACAGTGGAGC TGATGTGATT GGTCAGTCCT CCCTTCTTCC TCGATTTTCC
    ATTCAGGATA ACTCCTACCA GAACAACAGC ATTTTTAGTG AGGCCTACGG TAAAAACATG
    ACACCTTCAT CTAAGCCGGA TGCACCCATG ATGCATCAGG AGCCCTCACT CTATTCCCTG
    TTTGAAGGAA ATCCCTGGTC TCCCTCCCTT CCTGCCAGCT CAGATCACTC AACCCCAGCA
    AGCCAGTCCC CTCACTCCTC CAATCCCAGC AGTCTGCCTT CATCCCCACC CACCCACAGC
    CATGGCTCCA TGCCCTTCTC CAACTTTGGC CCCATCGGGA CGCCAGACAG CAGAGACCGC
    CGAGCAAATG ATCGCTGGAA GGCTGAGAAG ACTGGTGTGA GTGGCTTTGG GCTGGATTAC
    CTGCCTTCTG CCTCGACCTC GTCTGTTCCA GAGACCAACA GCTGGCACCA GGGGGCGCCA
    ACCAGCACCT GGGCAGCCCA GGACATGCCA ATGGAGGACT CCTCTACTGT CCTACTTGAC
    AGTTTCAAGT CAATCTGGTC GAGCTCCATG ATGCAGCCTG GTCCGTCTGC GCTGGAGCAG
    CTCCTGATGC AGCAGAAACA GAAGCAACAG CGCGGACACG GCAACATGAA CCCGCCACAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.