SMG7 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SMG7
Entrez Gene ID	9887
Full Name	SMG7, nonsense mediated mRNA decay factor
Synonyms	C1orf16,EST1C,SGA56M
General protein information	Preferred Names SMG7, nonsense mediated mRNA decay factor Names protein SMG7 EST1 telomerase component homolog C EST1-like protein C breast cancer-associated antigen SGA-56M ever shorter telomeres 1C smg-7 homolog, nonsense mediated mRNA decay factor
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1q25.3
Summary	This gene encodes a protein that is essential for nonsense-mediated mRNA decay (NMD); a process whereby transcripts with premature termination codons are targeted for rapid degradation by a mRNA decay complex. The mRNA decay complex consists, in part, of this protein along with proteins SMG5 and UPF1. The N-terminal domain of this protein is thought to mediate its association with SMG5 or UPF1 while the C-terminal domain interacts with the mRNA decay complex. This protein may therefore couple changes in UPF1 phosphorylation state to the degradation of NMD-candidate transcripts. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Aug 2011].
Disorder MIM:	610964

mRNA and Protein(s)

mRNA	Protein	Name
XM_011510206.2	XP_011508508.1	protein SMG7 isoform X3
XM_011510207.2	XP_011508509.1	protein SMG7 isoform X4
XM_011510208.2	XP_011508510.1	protein SMG7 isoform X5
XM_005245652.3	XP_005245709.1	protein SMG7 isoform X2
NM_201569.2	NP_963863.2	protein SMG7 isoform 4
XM_017002969.1	XP_016858458.1	protein SMG7 isoform X10
NM_001331007.2	NP_001317936.1	protein SMG7 isoform 6
NM_201568.3	NP_963862.1	protein SMG7 isoform 2
NM_001350221.1	NP_001337150.1	protein SMG7 isoform 6
NM_001350220.1	NP_001337149.1	protein SMG7 isoform 8
NM_001331007.1	NP_001317936.1	protein SMG7 isoform 6
XM_017002968.1	XP_016858457.1	protein SMG7 isoform X9
XM_011510205.2	XP_011508507.1	protein SMG7 isoform X1
NM_001350221.2	NP_001337150.1	protein SMG7 isoform 6
NM_001350222.2	NP_001337151.1	protein SMG7 isoform 9
XM_011510206.3	XP_011508508.1	protein SMG7 isoform X3
XM_011510207.3	XP_011508509.1	protein SMG7 isoform X4
XM_011510210.2	XP_011508512.1	protein SMG7 isoform X8
NM_001350219.1	NP_001337148.1	protein SMG7 isoform 7
NM_201568.2	NP_963862.1	protein SMG7 isoform 2
NM_001174061.1	NP_001167532.1	protein SMG7 isoform 5
XM_005245653.4	XP_005245710.1	protein SMG7 isoform X4
NM_001174061.2	NP_001167532.1	protein SMG7 isoform 5
NM_001350220.2	NP_001337149.1	protein SMG7 isoform 8
NM_001375584.1	NP_001362513.1	protein SMG7 isoform 10
NM_001375585.1	NP_001362514.1	protein SMG7 isoform 11
XM_017002968.2	XP_016858457.1	protein SMG7 isoform X9
XM_005245649.3	XP_005245706.1	protein SMG7 isoform X1
NM_173156.2	NP_775179.1	protein SMG7 isoform 1
XM_006711678.3	XP_006711741.1	protein SMG7 isoform X6
NM_001350219.2	NP_001337148.1	protein SMG7 isoform 7
NM_201569.3	NP_963863.2	protein SMG7 isoform 4
NM_173156.3	NP_775179.1	protein SMG7 isoform 1
XM_011510205.3	XP_011508507.1	protein SMG7 isoform X1
XM_005245649.4	XP_005245706.1	protein SMG7 isoform X1
XM_011510208.3	XP_011508510.1	protein SMG7 isoform X5
XM_011510209.3	XP_011508511.1	protein SMG7 isoform X7
NM_001350222.1	NP_001337151.1	protein SMG7 isoform 9
XM_011510209.2	XP_011508511.1	protein SMG7 isoform X7
XM_005245653.5	XP_005245710.1	protein SMG7 isoform X4
XM_017002970.1	XP_016858459.1	protein SMG7 isoform X10

Pathways from BioSystems

Homologies

	SMG7	XP_002802068.1
Mus musculus (house mouse)	Smg7	NP_001005507.1
Danio rerio (zebrafish)	smg7	NP_001038548.2
Bos taurus (cattle)	SMG7	NP_001180134.2
Xenopus tropicalis (tropical clawed frog)	smg7	NP_989258.1
Homo sapiens (human)	SMG7	NP_775179.1
Pan troglodytes (chimpanzee)	SMG7	XP_514048.3
Gallus gallus (chicken)	SMG7	XP_004943329.1
Canis lupus familiaris (dog)	SMG7	XP_537157.4
Rattus norvegicus (Norway rat)	Smg7	NP_001178478.1

Gene Ontology
Bibliography

Related articles in PubMed

Transcript-specific characteristics determine the contribution of endo- and exonucleolytic decay pathways during the degradation of nonsense-mediated decay substrates.
Ottens F, Boehm V, Sibley CR, Ule J, Gehring NH
RNA (New York, N.Y.)23(8)1224-1236(2017 08)

Transcriptome-wide identification of NMD-targeted human mRNAs reveals extensive redundancy between SMG6- and SMG7-mediated degradation pathways.
Colombo M, Karousis ED, Bourquin J, Bruggmann R, M?hlemann O
RNA (New York, N.Y.)23(2)189-201(2017 02)

Frameshift Mutations of SMG7 Essential for Nonsense-Mediated mRNA Decay in Gastric and Colorectal Cancers.
Jo YS, Song SY, Kim MS, Yoo NJ, Lee SH
Pathology oncology research : POR23(1)221-222(2017 Jan)

Decreased SMG7 expression associates with lupus-risk variants and elevated antinuclear antibody production.
Deng Y, Zhao J, Sakurai D, Sestak AL, Osadchiy V, Langefeld CD, Kaufman KM, Kelly JA, James JA, Petri MA, Bae SC, Alarc?n-Riquelme ME, Alarc?n GS, Anaya JM, Criswell LA, Freedman BI, Kamen DL, Gilkeson GS, Jacob CO, Merrill JT, Gaffney PM, Sivils KM, Niewold TB, Ramsey-Goldman R, Reveille JD, Scofield RH, Stevens AM, Boackle SA, Vil? LM, Sohn II, Lee S, Chang DM, Song YW, Vyse TJ, Harley JB, Brown EE, Edberg JC, Kimberly RP, Cantor RM, Hahn BH, Grossman JM, Tsao BP
Annals of the rheumatic diseases75(11)2007-2013(2016 Nov)

The host nonsense-mediated mRNA decay pathway restricts Mammalian RNA virus replication.
Balistreri G, Horvath P, Schweingruber C, Z?nd D, McInerney G, Merits A, M?hlemann O, Azzalin C, Helenius A
Cell host & microbe16(3)403-11(2014 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Nonsense-mediated mRNA decay (NMD) substrates with PTCs undergo constitutive SMG6-dependent endocleavage, rather than SMG7-dependent exonucleolytic decay. In contrast, the turnover of NMD substrates containing upstream ORFs and long 3' UTRs involves both SMG6- and SMG7-dependent endo- and exonucleolytic decay, respectively; extent to which SMG6 and SMG7 degrade NMD substrates is determined by the mRNA architecture.
Title: Transcript-specific characteristics determine the contribution of endo- and exonucleolytic decay pathways during the degradation of nonsense-mediated decay substrates.

Transcriptome-wide identification of nonsense-mediated mRNA decay-targeted human mRNAs reveals extensive redundancy between SMG6- and SMG7-mediated degradation pathways.
Title: Transcriptome-wide identification of NMD-targeted human mRNAs reveals extensive redundancy between SMG6- and SMG7-mediated degradation pathways.

In the Title.
Title: Frameshift Mutations of SMG7 Essential for Nonsense-Mediated mRNA Decay in Gastric and Colorectal Cancers.

We showed SMG7 mRNA levels in peripheral blood mononuclear cells correlated inversely with antinuclear antibody titres of patients with systemic lupus erythematosus. The inverse relationship between levels of the risk allele-associated SMG7 mRNAs and antinuclear antibody suggested the novel contribution of mRNA surveillance pathway to systemic lupus erythematosus pathogenesis.
Title: Decreased SMG7 expression associates with lupus-risk variants and elevated antinuclear antibody production.

Depletion of nonsense-mediated mRNA decay pathway components Upf1, Smg5, and Smg7 led to increased levels of viral proteins and and virus release.
Title: The host nonsense-mediated mRNA decay pathway restricts Mammalian RNA virus replication.

The following SMG7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SMG7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu04620	NM_001174061.2 Latest version!	Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$399.50-$559.30 $799.00
OHu63457	XM_017002970.1 Latest version!	Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu63457	XM_017002969.1 Latest version!	Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu102343	NM_001350222.2 Latest version!	Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu34882	NM_001375584.1 Latest version!	Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu34886	NM_001375585.1 Latest version!	Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu90882	XM_017002968.2 Latest version!	Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu02898	NM_201568.3 Latest version!	Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu07205	NM_173156.3 Latest version!	Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu22107	NM_201569.3 Latest version!	Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu34879	NM_001350220.2 Latest version!	Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu34883	NM_001350221.2 Latest version!	Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu34883	NM_001331007.2 Latest version!	Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu63455	NM_001350219.2 Latest version!	Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu34879	XM_011510205.3 Latest version!	Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu34879	XM_005245649.4 Latest version!	Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu34882	XM_005245652.3 Latest version!	Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu34883	XM_011510206.3 Latest version!	Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu34884	XM_011510207.3 Latest version!	Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu34884	XM_005245653.5 Latest version!	Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu34886	XM_006711678.3 Latest version!	Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu63454	XM_011510208.3 Latest version!	Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu63455	XM_011510209.3 Latest version!	Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu63456	XM_011510210.2 Latest version!	Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
View Old Accession Versions >>
OHu34883	NM_001331007.1	Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu34883	NM_001350221.1	Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu34883	XM_011510206.2	Homo sapiens SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu34884	XM_011510207.2	Homo sapiens SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu02898	NM_201568.2	Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu04620	NM_001174061.1	Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$399.50-$559.30 $799.00
OHu34879	NM_001350220.1	Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu90882	XM_017002968.1	Homo sapiens SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu63455	NM_001350219.1	Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu22107	NM_201569.2	Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu07205	NM_173156.2	Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu34879	XM_011510205.2	Homo sapiens SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu34879	XM_005245649.3	Homo sapiens SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu63454	XM_011510208.2	Homo sapiens SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu102343	NM_001350222.1	Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu63455	XM_011510209.2	Homo sapiens SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu34884	XM_005245653.4	Homo sapiens SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SMG7 ( NM_001174061.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001167532.1 Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 5, mRNA.

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CloneID	OHu04620
Clone ID Related Accession (Same CDS sequence)	NM_001174061.1 , NM_001174061.2
Accession Version	NM_001174061.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3438bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-27
Organism	Homo sapiens(human)
Product	protein SMG7 isoform 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB223038.1, AK299178.1, AB085674.1 and CN479993.1. Transcript Variant: This variant (5) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. This variant also contains an alternate in-frame exon in the 3' coding region, compared to variant 1. The encoded isoform (5) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK299178.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTAGTTA CCGATTTGGA ATACGCTTTA GACAAGAAAG TAGAACAGGA TCTCTGGAAT 
CACGCCTTTA AGAATCAGAT CACAACACTG CAAGGCCAGG CAAAGAATCG AGCAAATCCG 
AATCGGAGTG AAGTTCAGGC AAACCTTTCT CTGTTCCTAG AGGCAGCTAG TGGCTTCTAT 
ACTCAGTTAT TACAAGAACT GTGTACAGTA TTTAATGTAG ATTTACCATG CCGTGTGAAG 
TCTTCCCAAT TGGGAATTAT CAGCAATAAA CAGACGCATA CCAGCGCCAT AGTGAAGCCA 
CAGTCTAGCT CCTGTTCCTA TATCTGCCAG CACTGCCTCG TCCACCTTGG AGACATTGCT 
CGATACAGAA ACCAGACCAG CCAGGCAGAG TCCTACTATA GGCATGCAGC TCAGCTTGTC 
CCCTCCAATG GTCAGCCTTA TAATCAGTTG GCTATCTTAG CTTCTTCCAA AGGAGACCAT 
CTGACCACAA TTTTCTACTA CTGCAGAAGC ATTGCTGTGA AGTTCCCTTT CCCAGCTGCC 
TCCACTAATC TGCAAAAAGC ACTTTCTAAA GCACTGGAAA GCCGAGATGA GGTGAAAACC 
AAGTGGGGTG TTTCTGACTT CATCAAGGCC TTTATTAAAT TCCACGGTCA TGTGTACCTG 
AGTAAGAGCT TGGAAAAGTT GAGCCCTCTT CGAGAGAAAT TGGAAGAACA GTTTAAGAGG 
CTGCTATTCC AAAAAGCTTT CAACTCTCAG CAGTTAGTTC ATGTCACTGT CATTAACCTG 
TTTCAACTTC ATCACCTTCG TGACTTTAGC AATGAAACCG AGCAGCACAC TTATAGCCAA 
GATGAGCAGC TATGTTGGAC ACAGTTGCTG GCCCTCTTTA TGTCTTTTCT TGGCATCCTG 
TGCAAGTGTC CTCTACAGAA TGAGTCTCAG GAGGAGTCCT ACAATGCCTA TCCTCTTCCA 
GCAGTCAAGG TCTCCATGGA CTGGCTAAGA CTCAGACCCA GGGTCTTTCA GGAGGCAGTG 
GTGGATGAAA GACAGTACAT TTGGCCCTGG TTGATTTCTC TTCTGAATAG TTTCCATCCC 
CATGAAGAGG ACCTCTCAAG TATTAGTGCG ACACCACTTC CAGAGGAGTT TGAATTACAA 
GGATTTTTGG CATTGAGACC TTCTTTCAGG AACTTGGATT TTTCCAAAGG TCACCAGGGT 
ATTACAGGGG ACAAAGAAGG CCAGCAACGA CGAATACGAC AGCAACGCTT GATCTCTATA 
GGCAAATGGA TTGCTGATAA TCAGCCAAGG CTGATTCAGT GTGAAAATGA GGTAGGGAAA 
TTGTTGTTTA TCACAGAAAT CCCAGAATTA ATACTGGAAG ACCCCAGTGA AGCCAAAGAG 
AACCTCATTC TGCAAGAAAC ATCTGTGATA GAGTCGCTGG CTGCAGATGG GAGCCCAGGG 
CTAAAATCAG TGCTATCTAC AAGCCGAAAT TTAAGCAACA ACTGTGACAC AGGAGAGAAG 
CCAGTGGTTA CCTTCAAAGA AAACATTAAG ACACGAGAAG TGAACAGAGA CCAAGGAAGA 
AGTTTTCCTC CCAAAGAGGT GAGAAGGGAC TATAGCAAAG GAATAACTGT AACTAAGAAT 
GATGGAAAGA AGGACAACAA CAAGAGGAAA ACTGAAACCA AGAAATGCAC CTTAGAAAAG 
TTACAGGAAA CAGGAAAGCA GAATGTGGCA GTGCAGGTAA AATCCCAGAC AGAACTAAGA 
AAGACTCCAG TGTCTGAAGC CAGAAAAACA CCTGTAACTC AAACCCCAAC TCAAGCAAGT 
AACTCCCAGT TCATCCCCAT TCATCACCCT GGAGCCTTCC CTCCTCTTCC CAGCAGGCCA 
GGGTTTCCGC CCCCAACATA TGTTATCCCC CCGCCTGTGG CATTTTCTAT GGGCTCAGGT 
TACACCTTCC CAGCTGGTGT TTCTGTCCCA GGAACCTTTC TTCAGCCTAC AGCTCACTCT 
CCAGCAGGAA ACCAGGTGCA AGCTGGGAAA CAGTCCCACA TTCCTTACAG CCAGCAACGG 
CCCTCTGGAC CAGGGCCAAT GAACCAGGGA CCTCAACAAT CACAGCCACC TTCCCAGCAA 
CCCCTTACAT CTTTACCAGC TCAGCCAACA GCACAGTCTA CAAGCCAGCT GCAGGTTCAA 
GCTCTAACTC AGCAACAACA ATCCCCTACA AAAGCTGTGC CGGCTTTGGG GAAAAGCCCG 
CCTCACCACT CTGGATTCCA GCAGTATCAA CAGGCAGATG CCTCCAAACA GCTGTGGAAT 
CCCCCTCAGG TTCAAGGCCC ATTAGGGAAA ATTATGCCTG TGAAACAGCC CTACTACCTT 
CAGACCCAAG ACCCCATAAA ACTGTTTGAG CCGTCATTGC AACCTCCTGT AATGCAGCAG 
CAGCCTCTAG AAAAAAAAAT GAAGCCTTTT CCCATGGAGC CATATAACCA TAATCCCTCA 
GAAGTCAAGG TCCCAGAATT CTACTGGGAT TCTTCCTACA GCATGGCTGA TAACAGATCT 
GTAATGGCAC AGCAAGCAAA CATAGACCGC AGGGGCAAAC GGTCACCAGG AGTCTTCCGT 
CCAGAGCAGG ATCCTGTACC CAGAATGCCG TTTGAGGACC CCAAGAGCTC CCCTCTGCTT 
CCTCCGGACC TGTTAAAGAG TCTGGCTGCC TTGGAGGAAG AGGAAGAGCT GATTTTTTCT 
AACCCTCCTG ATCTTTACCC GGCTCTGCTG GGGCCTCTCG CCTCTCTTCC TGGACGAAGC 
CTTTTTAAAT CCTTATTGGA GAAGCCCTCA GAGCTCATGT CACATTCATC CTCTTTCCTG 
TCCCTCACCG GATTCTCTCT CAATCAGGAA AGATACCCAA ATAATAGTAT GTTCAATGAG 
GTATATGGGA AAAACCTGAC ATCCAGCTCC AAAGCAGAAC TCAGTCCCTC AATGGCCCCC 
CAGGAAACAT CTCTGTATTC CCTTTTTGAA GGGACTCCGT GGTCTCCATC ACTTCCTGCC 
AGTTCAGATC ATTCAACACC AGCCAGCCAG TCTCCTCATT CCTCTAACCC AAGCAGCCTA 
CCCAGCTCTC CTCCAACACA CAACCATAAT TCTGTTCCAT TCTCCAATTT TGGACCCATT 
GGGACTCCAG ATAACAGGGA TAGAAGGACT GCAGATCGGT GGAAAACTGA TAAGCCAGCC 
ATGGGTGGGT TTGGCATTGA TTATCTCTCA GCAACGTCAT CCTCTGAGAG CAGTTGGCAT 
CAGGCCAGCA CTCCGAGTGG CACCTGGACA GGCCATGGCC CTTCCATGGA GGATTCCTCT 
GCTGTCCTCA TGGAAAGCCT AAAGTCTATC TGGTCCAGTT CCATGATGCA TCCTGGACCT 
TCTGCTCTGG AGCAGCTGTT AATGCAGCAG AAGCAGAAAC AGCAACGGGG ACAAGGCACC 
ATGAACCCTC CACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001167532.1
CDS	345..3782
Translation	MLVTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRANPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQTHTSAIVKPQSSSCSYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNLQKALSKALESRDEVKTKWGVSDFIKAFIKFHGHVYLSKSLEKLSPLREKLEEQFKRLLFQKAFNSQQLVHVTVINLFQLHHLRDFSNETEQHTYSQDEQLCWTQLLALFMSFLGILCKCPLQNESQEESYNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPHEEDLSSISATPLPEEFELQGFLALRPSFRNLDFSKGHQGITGDKEGQQRRIRQQRLISIGKWIADNQPRLIQCENEVGKLLFITEIPELILEDPSEAKENLILQETSVIESLAADGSPGLKSVLSTSRNLSNNCDTGEKPVVTFKENIKTREVNRDQGRSFPPKEVRRDYSKGITVTKNDGKKDNNKRKTETKKCTLEKLQETGKQNVAVQVKSQTELRKTPVSEARKTPVTQTPTQASNSQFIPIHHPGAFPPLPSRPGFPPPTYVIPPPVAFSMGSGYTFPAGVSVPGTFLQPTAHSPAGNQVQAGKQSHIPYSQQRPSGPGPMNQGPQQSQPPSQQPLTSLPAQPTAQSTSQLQVQALTQQQQSPTKAVPALGKSPPHHSGFQQYQQADASKQLWNPPQVQGPLGKIMPVKQPYYLQTQDPIKLFEPSLQPPVMQQQPLEKKMKPFPMEPYNHNPSEVKVPEFYWDSSYSMADNRSVMAQQANIDRRGKRSPGVFRPEQDPVPRMPFEDPKSSPLLPPDLLKSLAALEEEEELIFSNPPDLYPALLGPLASLPGRSLFKSLLEKPSELMSHSSSFLSLTGFSLNQERYPNNSMFNEVYGKNLTSSSKAELSPSMAPQETSLYSLFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSVPFSNFGPIGTPDNRDRRTADRWKTDKPAMGGFGIDYLSATSSSESSWHQASTPSGTWTGHGPSMEDSSAVLMESLKSIWSSSMMHPGPSALEQLLMQQKQKQQRGQGTMNPPH

Target ORF information:

RefSeq Version	NM_001174061.1
Organism	Homo sapiens(human)
Definition	Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001174061.1

ORF Insert Sequence:

ATGCTAGTTA CCGATTTGGA ATACGCTTTA GACAAGAAAG TAGAACAGGA TCTCTGGAAT 
CACGCCTTTA AGAATCAGAT CACAACACTG CAAGGCCAGG CAAAGAATCG AGCAAATCCG 
AATCGGAGTG AAGTTCAGGC AAACCTTTCT CTGTTCCTAG AGGCAGCTAG TGGCTTCTAT 
ACTCAGTTAT TACAAGAACT GTGTACAGTA TTTAATGTAG ATTTACCATG CCGTGTGAAG 
TCTTCCCAAT TGGGAATTAT CAGCAATAAA CAGACGCATA CCAGCGCCAT AGTGAAGCCA 
CAGTCTAGCT CCTGTTCCTA TATCTGCCAG CACTGCCTCG TCCACCTTGG AGACATTGCT 
CGATACAGAA ACCAGACCAG CCAGGCAGAG TCCTACTATA GGCATGCAGC TCAGCTTGTC 
CCCTCCAATG GTCAGCCTTA TAATCAGTTG GCTATCTTAG CTTCTTCCAA AGGAGACCAT 
CTGACCACAA TTTTCTACTA CTGCAGAAGC ATTGCTGTGA AGTTCCCTTT CCCAGCTGCC 
TCCACTAATC TGCAAAAAGC ACTTTCTAAA GCACTGGAAA GCCGAGATGA GGTGAAAACC 
AAGTGGGGTG TTTCTGACTT CATCAAGGCC TTTATTAAAT TCCACGGTCA TGTGTACCTG 
AGTAAGAGCT TGGAAAAGTT GAGCCCTCTT CGAGAGAAAT TGGAAGAACA GTTTAAGAGG 
CTGCTATTCC AAAAAGCTTT CAACTCTCAG CAGTTAGTTC ATGTCACTGT CATTAACCTG 
TTTCAACTTC ATCACCTTCG TGACTTTAGC AATGAAACCG AGCAGCACAC TTATAGCCAA 
GATGAGCAGC TATGTTGGAC ACAGTTGCTG GCCCTCTTTA TGTCTTTTCT TGGCATCCTG 
TGCAAGTGTC CTCTACAGAA TGAGTCTCAG GAGGAGTCCT ACAATGCCTA TCCTCTTCCA 
GCAGTCAAGG TCTCCATGGA CTGGCTAAGA CTCAGACCCA GGGTCTTTCA GGAGGCAGTG 
GTGGATGAAA GACAGTACAT TTGGCCCTGG TTGATTTCTC TTCTGAATAG TTTCCATCCC 
CATGAAGAGG ACCTCTCAAG TATTAGTGCG ACACCACTTC CAGAGGAGTT TGAATTACAA 
GGATTTTTGG CATTGAGACC TTCTTTCAGG AACTTGGATT TTTCCAAAGG TCACCAGGGT 
ATTACAGGGG ACAAAGAAGG CCAGCAACGA CGAATACGAC AGCAACGCTT GATCTCTATA 
GGCAAATGGA TTGCTGATAA TCAGCCAAGG CTGATTCAGT GTGAAAATGA GGTAGGGAAA 
TTGTTGTTTA TCACAGAAAT CCCAGAATTA ATACTGGAAG ACCCCAGTGA AGCCAAAGAG 
AACCTCATTC TGCAAGAAAC ATCTGTGATA GAGTCGCTGG CTGCAGATGG GAGCCCAGGG 
CTAAAATCAG TGCTATCTAC AAGCCGAAAT TTAAGCAACA ACTGTGACAC AGGAGAGAAG 
CCAGTGGTTA CCTTCAAAGA AAACATTAAG ACACGAGAAG TGAACAGAGA CCAAGGAAGA 
AGTTTTCCTC CCAAAGAGGT GAGAAGGGAC TATAGCAAAG GAATAACTGT AACTAAGAAT 
GATGGAAAGA AGGACAACAA CAAGAGGAAA ACTGAAACCA AGAAATGCAC CTTAGAAAAG 
TTACAGGAAA CAGGAAAGCA GAATGTGGCA GTGCAGGTAA AATCCCAGAC AGAACTAAGA 
AAGACTCCAG TGTCTGAAGC CAGAAAAACA CCTGTAACTC AAACCCCAAC TCAAGCAAGT 
AACTCCCAGT TCATCCCCAT TCATCACCCT GGAGCCTTCC CTCCTCTTCC CAGCAGGCCA 
GGGTTTCCGC CCCCAACATA TGTTATCCCC CCGCCTGTGG CATTTTCTAT GGGCTCAGGT 
TACACCTTCC CAGCTGGTGT TTCTGTCCCA GGAACCTTTC TTCAGCCTAC AGCTCACTCT 
CCAGCAGGAA ACCAGGTGCA AGCTGGGAAA CAGTCCCACA TTCCTTACAG CCAGCAACGG 
CCCTCTGGAC CAGGGCCAAT GAACCAGGGA CCTCAACAAT CACAGCCACC TTCCCAGCAA 
CCCCTTACAT CTTTACCAGC TCAGCCAACA GCACAGTCTA CAAGCCAGCT GCAGGTTCAA 
GCTCTAACTC AGCAACAACA ATCCCCTACA AAAGCTGTGC CGGCTTTGGG GAAAAGCCCG 
CCTCACCACT CTGGATTCCA GCAGTATCAA CAGGCAGATG CCTCCAAACA GCTGTGGAAT 
CCCCCTCAGG TTCAAGGCCC ATTAGGGAAA ATTATGCCTG TGAAACAGCC CTACTACCTT 
CAGACCCAAG ACCCCATAAA ACTGTTTGAG CCGTCATTGC AACCTCCTGT AATGCAGCAG 
CAGCCTCTAG AAAAAAAAAT GAAGCCTTTT CCCATGGAGC CATATAACCA TAATCCCTCA 
GAAGTCAAGG TCCCAGAATT CTACTGGGAT TCTTCCTACA GCATGGCTGA TAACAGATCT 
GTAATGGCAC AGCAAGCAAA CATAGACCGC AGGGGCAAAC GGTCACCAGG AGTCTTCCGT 
CCAGAGCAGG ATCCTGTACC CAGAATGCCG TTTGAGGACC CCAAGAGCTC CCCTCTGCTT 
CCTCCGGACC TGTTAAAGAG TCTGGCTGCC TTGGAGGAAG AGGAAGAGCT GATTTTTTCT 
AACCCTCCTG ATCTTTACCC GGCTCTGCTG GGGCCTCTCG CCTCTCTTCC TGGACGAAGC 
CTTTTTAAAT CCTTATTGGA GAAGCCCTCA GAGCTCATGT CACATTCATC CTCTTTCCTG 
TCCCTCACCG GATTCTCTCT CAATCAGGAA AGATACCCAA ATAATAGTAT GTTCAATGAG 
GTATATGGGA AAAACCTGAC ATCCAGCTCC AAAGCAGAAC TCAGTCCCTC AATGGCCCCC 
CAGGAAACAT CTCTGTATTC CCTTTTTGAA GGGACTCCGT GGTCTCCATC ACTTCCTGCC 
AGTTCAGATC ATTCAACACC AGCCAGCCAG TCTCCTCATT CCTCTAACCC AAGCAGCCTA 
CCCAGCTCTC CTCCAACACA CAACCATAAT TCTGTTCCAT TCTCCAATTT TGGACCCATT 
GGGACTCCAG ATAACAGGGA TAGAAGGACT GCAGATCGGT GGAAAACTGA TAAGCCAGCC 
ATGGGTGGGT TTGGCATTGA TTATCTCTCA GCAACGTCAT CCTCTGAGAG CAGTTGGCAT 
CAGGCCAGCA CTCCGAGTGG CACCTGGACA GGCCATGGCC CTTCCATGGA GGATTCCTCT 
GCTGTCCTCA TGGAAAGCCT AAAGTCTATC TGGTCCAGTT CCATGATGCA TCCTGGACCT 
TCTGCTCTGG AGCAGCTGTT AATGCAGCAG AAGCAGAAAC AGCAACGGGG ACAAGGCACC 
ATGAACCCTC CACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG7 ( NM_001174061.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001167532.1 Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 5, mRNA.

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CloneID	OHu04620
Clone ID Related Accession (Same CDS sequence)	NM_001174061.1 , NM_001174061.2
Accession Version	NM_001174061.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3438bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-28
Organism	Homo sapiens(human)
Product	protein SMG7 isoform 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB223038.1, AK299178.1, AB085674.1 and CN479993.1. On Jun 2, 2019 this sequence version replaced NM_001174061.1. Transcript Variant: This variant (5) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. This variant also contains an alternate in-frame exon in the 3' coding region, compared to variant 1. The encoded isoform (5) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK299178.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTAGTTA CCGATTTGGA ATACGCTTTA GACAAGAAAG TAGAACAGGA TCTCTGGAAT 
CACGCCTTTA AGAATCAGAT CACAACACTG CAAGGCCAGG CAAAGAATCG AGCAAATCCG 
AATCGGAGTG AAGTTCAGGC AAACCTTTCT CTGTTCCTAG AGGCAGCTAG TGGCTTCTAT 
ACTCAGTTAT TACAAGAACT GTGTACAGTA TTTAATGTAG ATTTACCATG CCGTGTGAAG 
TCTTCCCAAT TGGGAATTAT CAGCAATAAA CAGACGCATA CCAGCGCCAT AGTGAAGCCA 
CAGTCTAGCT CCTGTTCCTA TATCTGCCAG CACTGCCTCG TCCACCTTGG AGACATTGCT 
CGATACAGAA ACCAGACCAG CCAGGCAGAG TCCTACTATA GGCATGCAGC TCAGCTTGTC 
CCCTCCAATG GTCAGCCTTA TAATCAGTTG GCTATCTTAG CTTCTTCCAA AGGAGACCAT 
CTGACCACAA TTTTCTACTA CTGCAGAAGC ATTGCTGTGA AGTTCCCTTT CCCAGCTGCC 
TCCACTAATC TGCAAAAAGC ACTTTCTAAA GCACTGGAAA GCCGAGATGA GGTGAAAACC 
AAGTGGGGTG TTTCTGACTT CATCAAGGCC TTTATTAAAT TCCACGGTCA TGTGTACCTG 
AGTAAGAGCT TGGAAAAGTT GAGCCCTCTT CGAGAGAAAT TGGAAGAACA GTTTAAGAGG 
CTGCTATTCC AAAAAGCTTT CAACTCTCAG CAGTTAGTTC ATGTCACTGT CATTAACCTG 
TTTCAACTTC ATCACCTTCG TGACTTTAGC AATGAAACCG AGCAGCACAC TTATAGCCAA 
GATGAGCAGC TATGTTGGAC ACAGTTGCTG GCCCTCTTTA TGTCTTTTCT TGGCATCCTG 
TGCAAGTGTC CTCTACAGAA TGAGTCTCAG GAGGAGTCCT ACAATGCCTA TCCTCTTCCA 
GCAGTCAAGG TCTCCATGGA CTGGCTAAGA CTCAGACCCA GGGTCTTTCA GGAGGCAGTG 
GTGGATGAAA GACAGTACAT TTGGCCCTGG TTGATTTCTC TTCTGAATAG TTTCCATCCC 
CATGAAGAGG ACCTCTCAAG TATTAGTGCG ACACCACTTC CAGAGGAGTT TGAATTACAA 
GGATTTTTGG CATTGAGACC TTCTTTCAGG AACTTGGATT TTTCCAAAGG TCACCAGGGT 
ATTACAGGGG ACAAAGAAGG CCAGCAACGA CGAATACGAC AGCAACGCTT GATCTCTATA 
GGCAAATGGA TTGCTGATAA TCAGCCAAGG CTGATTCAGT GTGAAAATGA GGTAGGGAAA 
TTGTTGTTTA TCACAGAAAT CCCAGAATTA ATACTGGAAG ACCCCAGTGA AGCCAAAGAG 
AACCTCATTC TGCAAGAAAC ATCTGTGATA GAGTCGCTGG CTGCAGATGG GAGCCCAGGG 
CTAAAATCAG TGCTATCTAC AAGCCGAAAT TTAAGCAACA ACTGTGACAC AGGAGAGAAG 
CCAGTGGTTA CCTTCAAAGA AAACATTAAG ACACGAGAAG TGAACAGAGA CCAAGGAAGA 
AGTTTTCCTC CCAAAGAGGT GAGAAGGGAC TATAGCAAAG GAATAACTGT AACTAAGAAT 
GATGGAAAGA AGGACAACAA CAAGAGGAAA ACTGAAACCA AGAAATGCAC CTTAGAAAAG 
TTACAGGAAA CAGGAAAGCA GAATGTGGCA GTGCAGGTAA AATCCCAGAC AGAACTAAGA 
AAGACTCCAG TGTCTGAAGC CAGAAAAACA CCTGTAACTC AAACCCCAAC TCAAGCAAGT 
AACTCCCAGT TCATCCCCAT TCATCACCCT GGAGCCTTCC CTCCTCTTCC CAGCAGGCCA 
GGGTTTCCGC CCCCAACATA TGTTATCCCC CCGCCTGTGG CATTTTCTAT GGGCTCAGGT 
TACACCTTCC CAGCTGGTGT TTCTGTCCCA GGAACCTTTC TTCAGCCTAC AGCTCACTCT 
CCAGCAGGAA ACCAGGTGCA AGCTGGGAAA CAGTCCCACA TTCCTTACAG CCAGCAACGG 
CCCTCTGGAC CAGGGCCAAT GAACCAGGGA CCTCAACAAT CACAGCCACC TTCCCAGCAA 
CCCCTTACAT CTTTACCAGC TCAGCCAACA GCACAGTCTA CAAGCCAGCT GCAGGTTCAA 
GCTCTAACTC AGCAACAACA ATCCCCTACA AAAGCTGTGC CGGCTTTGGG GAAAAGCCCG 
CCTCACCACT CTGGATTCCA GCAGTATCAA CAGGCAGATG CCTCCAAACA GCTGTGGAAT 
CCCCCTCAGG TTCAAGGCCC ATTAGGGAAA ATTATGCCTG TGAAACAGCC CTACTACCTT 
CAGACCCAAG ACCCCATAAA ACTGTTTGAG CCGTCATTGC AACCTCCTGT AATGCAGCAG 
CAGCCTCTAG AAAAAAAAAT GAAGCCTTTT CCCATGGAGC CATATAACCA TAATCCCTCA 
GAAGTCAAGG TCCCAGAATT CTACTGGGAT TCTTCCTACA GCATGGCTGA TAACAGATCT 
GTAATGGCAC AGCAAGCAAA CATAGACCGC AGGGGCAAAC GGTCACCAGG AGTCTTCCGT 
CCAGAGCAGG ATCCTGTACC CAGAATGCCG TTTGAGGACC CCAAGAGCTC CCCTCTGCTT 
CCTCCGGACC TGTTAAAGAG TCTGGCTGCC TTGGAGGAAG AGGAAGAGCT GATTTTTTCT 
AACCCTCCTG ATCTTTACCC GGCTCTGCTG GGGCCTCTCG CCTCTCTTCC TGGACGAAGC 
CTTTTTAAAT CCTTATTGGA GAAGCCCTCA GAGCTCATGT CACATTCATC CTCTTTCCTG 
TCCCTCACCG GATTCTCTCT CAATCAGGAA AGATACCCAA ATAATAGTAT GTTCAATGAG 
GTATATGGGA AAAACCTGAC ATCCAGCTCC AAAGCAGAAC TCAGTCCCTC AATGGCCCCC 
CAGGAAACAT CTCTGTATTC CCTTTTTGAA GGGACTCCGT GGTCTCCATC ACTTCCTGCC 
AGTTCAGATC ATTCAACACC AGCCAGCCAG TCTCCTCATT CCTCTAACCC AAGCAGCCTA 
CCCAGCTCTC CTCCAACACA CAACCATAAT TCTGTTCCAT TCTCCAATTT TGGACCCATT 
GGGACTCCAG ATAACAGGGA TAGAAGGACT GCAGATCGGT GGAAAACTGA TAAGCCAGCC 
ATGGGTGGGT TTGGCATTGA TTATCTCTCA GCAACGTCAT CCTCTGAGAG CAGTTGGCAT 
CAGGCCAGCA CTCCGAGTGG CACCTGGACA GGCCATGGCC CTTCCATGGA GGATTCCTCT 
GCTGTCCTCA TGGAAAGCCT AAAGTCTATC TGGTCCAGTT CCATGATGCA TCCTGGACCT 
TCTGCTCTGG AGCAGCTGTT AATGCAGCAG AAGCAGAAAC AGCAACGGGG ACAAGGCACC 
ATGAACCCTC CACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001167532.1
CDS	217..3654
Translation	MLVTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRANPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQTHTSAIVKPQSSSCSYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNLQKALSKALESRDEVKTKWGVSDFIKAFIKFHGHVYLSKSLEKLSPLREKLEEQFKRLLFQKAFNSQQLVHVTVINLFQLHHLRDFSNETEQHTYSQDEQLCWTQLLALFMSFLGILCKCPLQNESQEESYNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPHEEDLSSISATPLPEEFELQGFLALRPSFRNLDFSKGHQGITGDKEGQQRRIRQQRLISIGKWIADNQPRLIQCENEVGKLLFITEIPELILEDPSEAKENLILQETSVIESLAADGSPGLKSVLSTSRNLSNNCDTGEKPVVTFKENIKTREVNRDQGRSFPPKEVRRDYSKGITVTKNDGKKDNNKRKTETKKCTLEKLQETGKQNVAVQVKSQTELRKTPVSEARKTPVTQTPTQASNSQFIPIHHPGAFPPLPSRPGFPPPTYVIPPPVAFSMGSGYTFPAGVSVPGTFLQPTAHSPAGNQVQAGKQSHIPYSQQRPSGPGPMNQGPQQSQPPSQQPLTSLPAQPTAQSTSQLQVQALTQQQQSPTKAVPALGKSPPHHSGFQQYQQADASKQLWNPPQVQGPLGKIMPVKQPYYLQTQDPIKLFEPSLQPPVMQQQPLEKKMKPFPMEPYNHNPSEVKVPEFYWDSSYSMADNRSVMAQQANIDRRGKRSPGVFRPEQDPVPRMPFEDPKSSPLLPPDLLKSLAALEEEEELIFSNPPDLYPALLGPLASLPGRSLFKSLLEKPSELMSHSSSFLSLTGFSLNQERYPNNSMFNEVYGKNLTSSSKAELSPSMAPQETSLYSLFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSVPFSNFGPIGTPDNRDRRTADRWKTDKPAMGGFGIDYLSATSSSESSWHQASTPSGTWTGHGPSMEDSSAVLMESLKSIWSSSMMHPGPSALEQLLMQQKQKQQRGQGTMNPPH

Target ORF information:

RefSeq Version	NM_001174061.2
Organism	Homo sapiens(human)
Definition	Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001174061.2

ORF Insert Sequence:

ATGCTAGTTA CCGATTTGGA ATACGCTTTA GACAAGAAAG TAGAACAGGA TCTCTGGAAT 
CACGCCTTTA AGAATCAGAT CACAACACTG CAAGGCCAGG CAAAGAATCG AGCAAATCCG 
AATCGGAGTG AAGTTCAGGC AAACCTTTCT CTGTTCCTAG AGGCAGCTAG TGGCTTCTAT 
ACTCAGTTAT TACAAGAACT GTGTACAGTA TTTAATGTAG ATTTACCATG CCGTGTGAAG 
TCTTCCCAAT TGGGAATTAT CAGCAATAAA CAGACGCATA CCAGCGCCAT AGTGAAGCCA 
CAGTCTAGCT CCTGTTCCTA TATCTGCCAG CACTGCCTCG TCCACCTTGG AGACATTGCT 
CGATACAGAA ACCAGACCAG CCAGGCAGAG TCCTACTATA GGCATGCAGC TCAGCTTGTC 
CCCTCCAATG GTCAGCCTTA TAATCAGTTG GCTATCTTAG CTTCTTCCAA AGGAGACCAT 
CTGACCACAA TTTTCTACTA CTGCAGAAGC ATTGCTGTGA AGTTCCCTTT CCCAGCTGCC 
TCCACTAATC TGCAAAAAGC ACTTTCTAAA GCACTGGAAA GCCGAGATGA GGTGAAAACC 
AAGTGGGGTG TTTCTGACTT CATCAAGGCC TTTATTAAAT TCCACGGTCA TGTGTACCTG 
AGTAAGAGCT TGGAAAAGTT GAGCCCTCTT CGAGAGAAAT TGGAAGAACA GTTTAAGAGG 
CTGCTATTCC AAAAAGCTTT CAACTCTCAG CAGTTAGTTC ATGTCACTGT CATTAACCTG 
TTTCAACTTC ATCACCTTCG TGACTTTAGC AATGAAACCG AGCAGCACAC TTATAGCCAA 
GATGAGCAGC TATGTTGGAC ACAGTTGCTG GCCCTCTTTA TGTCTTTTCT TGGCATCCTG 
TGCAAGTGTC CTCTACAGAA TGAGTCTCAG GAGGAGTCCT ACAATGCCTA TCCTCTTCCA 
GCAGTCAAGG TCTCCATGGA CTGGCTAAGA CTCAGACCCA GGGTCTTTCA GGAGGCAGTG 
GTGGATGAAA GACAGTACAT TTGGCCCTGG TTGATTTCTC TTCTGAATAG TTTCCATCCC 
CATGAAGAGG ACCTCTCAAG TATTAGTGCG ACACCACTTC CAGAGGAGTT TGAATTACAA 
GGATTTTTGG CATTGAGACC TTCTTTCAGG AACTTGGATT TTTCCAAAGG TCACCAGGGT 
ATTACAGGGG ACAAAGAAGG CCAGCAACGA CGAATACGAC AGCAACGCTT GATCTCTATA 
GGCAAATGGA TTGCTGATAA TCAGCCAAGG CTGATTCAGT GTGAAAATGA GGTAGGGAAA 
TTGTTGTTTA TCACAGAAAT CCCAGAATTA ATACTGGAAG ACCCCAGTGA AGCCAAAGAG 
AACCTCATTC TGCAAGAAAC ATCTGTGATA GAGTCGCTGG CTGCAGATGG GAGCCCAGGG 
CTAAAATCAG TGCTATCTAC AAGCCGAAAT TTAAGCAACA ACTGTGACAC AGGAGAGAAG 
CCAGTGGTTA CCTTCAAAGA AAACATTAAG ACACGAGAAG TGAACAGAGA CCAAGGAAGA 
AGTTTTCCTC CCAAAGAGGT GAGAAGGGAC TATAGCAAAG GAATAACTGT AACTAAGAAT 
GATGGAAAGA AGGACAACAA CAAGAGGAAA ACTGAAACCA AGAAATGCAC CTTAGAAAAG 
TTACAGGAAA CAGGAAAGCA GAATGTGGCA GTGCAGGTAA AATCCCAGAC AGAACTAAGA 
AAGACTCCAG TGTCTGAAGC CAGAAAAACA CCTGTAACTC AAACCCCAAC TCAAGCAAGT 
AACTCCCAGT TCATCCCCAT TCATCACCCT GGAGCCTTCC CTCCTCTTCC CAGCAGGCCA 
GGGTTTCCGC CCCCAACATA TGTTATCCCC CCGCCTGTGG CATTTTCTAT GGGCTCAGGT 
TACACCTTCC CAGCTGGTGT TTCTGTCCCA GGAACCTTTC TTCAGCCTAC AGCTCACTCT 
CCAGCAGGAA ACCAGGTGCA AGCTGGGAAA CAGTCCCACA TTCCTTACAG CCAGCAACGG 
CCCTCTGGAC CAGGGCCAAT GAACCAGGGA CCTCAACAAT CACAGCCACC TTCCCAGCAA 
CCCCTTACAT CTTTACCAGC TCAGCCAACA GCACAGTCTA CAAGCCAGCT GCAGGTTCAA 
GCTCTAACTC AGCAACAACA ATCCCCTACA AAAGCTGTGC CGGCTTTGGG GAAAAGCCCG 
CCTCACCACT CTGGATTCCA GCAGTATCAA CAGGCAGATG CCTCCAAACA GCTGTGGAAT 
CCCCCTCAGG TTCAAGGCCC ATTAGGGAAA ATTATGCCTG TGAAACAGCC CTACTACCTT 
CAGACCCAAG ACCCCATAAA ACTGTTTGAG CCGTCATTGC AACCTCCTGT AATGCAGCAG 
CAGCCTCTAG AAAAAAAAAT GAAGCCTTTT CCCATGGAGC CATATAACCA TAATCCCTCA 
GAAGTCAAGG TCCCAGAATT CTACTGGGAT TCTTCCTACA GCATGGCTGA TAACAGATCT 
GTAATGGCAC AGCAAGCAAA CATAGACCGC AGGGGCAAAC GGTCACCAGG AGTCTTCCGT 
CCAGAGCAGG ATCCTGTACC CAGAATGCCG TTTGAGGACC CCAAGAGCTC CCCTCTGCTT 
CCTCCGGACC TGTTAAAGAG TCTGGCTGCC TTGGAGGAAG AGGAAGAGCT GATTTTTTCT 
AACCCTCCTG ATCTTTACCC GGCTCTGCTG GGGCCTCTCG CCTCTCTTCC TGGACGAAGC 
CTTTTTAAAT CCTTATTGGA GAAGCCCTCA GAGCTCATGT CACATTCATC CTCTTTCCTG 
TCCCTCACCG GATTCTCTCT CAATCAGGAA AGATACCCAA ATAATAGTAT GTTCAATGAG 
GTATATGGGA AAAACCTGAC ATCCAGCTCC AAAGCAGAAC TCAGTCCCTC AATGGCCCCC 
CAGGAAACAT CTCTGTATTC CCTTTTTGAA GGGACTCCGT GGTCTCCATC ACTTCCTGCC 
AGTTCAGATC ATTCAACACC AGCCAGCCAG TCTCCTCATT CCTCTAACCC AAGCAGCCTA 
CCCAGCTCTC CTCCAACACA CAACCATAAT TCTGTTCCAT TCTCCAATTT TGGACCCATT 
GGGACTCCAG ATAACAGGGA TAGAAGGACT GCAGATCGGT GGAAAACTGA TAAGCCAGCC 
ATGGGTGGGT TTGGCATTGA TTATCTCTCA GCAACGTCAT CCTCTGAGAG CAGTTGGCAT 
CAGGCCAGCA CTCCGAGTGG CACCTGGACA GGCCATGGCC CTTCCATGGA GGATTCCTCT 
GCTGTCCTCA TGGAAAGCCT AAAGTCTATC TGGTCCAGTT CCATGATGCA TCCTGGACCT 
TCTGCTCTGG AGCAGCTGTT AATGCAGCAG AAGCAGAAAC AGCAACGGGG ACAAGGCACC 
ATGAACCCTC CACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG7 ( XM_017002969.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016858458.1 Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X12, mRNA.

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CloneID	OHu63457
Clone ID Related Accession (Same CDS sequence)	XM_017002969.1 , XM_017002970.1
Accession Version	XM_017002969.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2559bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein SMG7 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTTTC TTGGCATCCT GTGCAAGTGT CCTCTACAGA ATGAGTCTCA GGAGGAGTCC 
TACAATGCCT ATCCTCTTCC AGCAGTCAAG GTCTCCATGG ACTGGCTAAG ACTCAGACCC 
AGGGTCTTTC AGGAGGCAGT GGTGGATGAA AGACAGTACA TTTGGCCCTG GTTGATTTCT 
CTTCTGAATA GTTTCCATCC CCATGAAGAG GACCTCTCAA GTATTAGTGC GACACCACTT 
CCAGAGGAGT TTGAATTACA AGGATTTTTG GCATTGAGAC CTTCTTTCAG GAACTTGGAT 
TTTTCCAAAG GTCACCAGGG TATTACAGGG GACAAAGAAG GCCAGCAACG ACGAATACGA 
CAGCAACGCT TGATCTCTAT AGGCAAATGG ATTGCTGATA ATCAGCCAAG GCTGATTCAG 
TGTGAAAATG AGGTAGGGAA ATTGTTGTTT ATCACAGAAA TCCCAGAATT AATACTGGAA 
GACCCCAGTG AAGCCAAAGA GAACCTCATT CTGCAAGAAA CATCTGTGAT AGAGTCGCTG 
GCTGCAGATG GGAGCCCAGG GCTAAAATCA GTGCTATCTA CAAGCCGAAA TTTAAGCAAC 
AACTGTGACA CAGGAGAGAA GCCAGTGGTT ACCTTCAAAG AAAACATTAA GACACGAGAA 
GTGAACAGAG ACCAAGGAAG AAGTTTTCCT CCCAAAGAGG TGAGAAGGGA CTATAGCAAA 
GGAATAACTG TAACTAAGAA TGATGGAAAG AAGGACAACA ACAAGAGGAA AACTGAAACC 
AAGAAATGCA CCTTAGAAAA GTTACAGGAA ACAGGAAAGC AGAATGTGGC AGTGCAGGTA 
AAATCCCAGA CAGAACTAAG AAAGACTCCA GTGTCTGAAG CCAGAAAAAC ACCTGTAACT 
CAAACCCCAA CTCAAGCAAG TAACTCCCAG TTCATCCCCA TTCATCACCC TGGAGCCTTC 
CCTCCTCTTC CCAGCAGGCC AGGGTTTCCG CCCCCAACAT ATGTTATCCC CCCGCCTGTG 
GCATTTTCTA TGGGCTCAGG TTACACCTTC CCAGCTGGTG TTTCTGTCCC AGGAACCTTT 
CTTCAGCCTA CAGCTCACTC TCCAGCAGGA AACCAGGTGC AAGCTGGGAA ACAGTCCCAC 
ATTCCTTACA GCCAGCAACG GCCCTCTGGA CCAGGGCCAA TGAACCAGGG ACCTCAACAA 
TCACAGCCAC CTTCCCAGCA ACCCCTTACA TCTTTACCAG CTCAGCCAAC AGCACAGTCT 
ACAAGCCAGC TGCAGGTTCA AGCTCTAACT CAGCAACAAC AATCCCCTAC AAAAGCTGTG 
CCGGCTTTGG GGAAAAGCCC GCCTCACCAC TCTGGATTCC AGCAGTATCA ACAGGCAGAT 
GCCTCCAAAC AGCTGTGGAA TCCCCCTCAG GTTCAAGGCC CATTAGGGAA AATTATGCCT 
GTGAAACAGC CCTACTACCT TCAGACCCAA GACCCCATAA AACTGTTTGA GCCGTCATTG 
CAACCTCCTG TAATGCAGCA GCAGCCTCTA GAAAAAAAAA TGAAGCCTTT TCCCATGGAG 
CCATATAACC ATAATCCCTC AGAAGTCAAG GTCCCAGAAT TCTACTGGGA TTCTTCCTAC 
AGCATGGCTG ATAACAGATC TGTAATGGCA CAGCAAGCAA ACATAGACCG CAGGGGCAAA 
CGGTCACCAG GAGTCTTCCG TCCAGAGCAG GATCCTGTAC CCAGAATGCC GTTTGAGGAC 
CCCAAGAGCT CCCCTCTGCT TCCTCCGGAC CTGTTAAAGA GTCTGGCTGC CTTGGAGGAA 
GAGGAAGAGC TGATTTTTTC TAACCCTCCT GATCTTTACC CGGCTCTGCT GGGGCCTCTC 
GCCTCTCTTC CTGGACGAAG CCTTTTTAAA TCCTTATTGG AGAAGCCCTC AGAGCTCATG 
TCACATTCAT CCTCTTTCCT GTCCCTCACC GGATTCTCTC TCAATCAGGA AAGATACCCA 
AATAATAGTA TGTTCAATGA GGTATATGGG AAAAACCTGA CATCCAGCTC CAAAGCAGAA 
CTCAGTCCCT CAATGGCCCC CCAGGAAACA TCTCTGTATT CCCTTTTTGA AGGGACTCCG 
TGGTCTCCAT CACTTCCTGC CAGTTCAGAT CATTCAACAC CAGCCAGCCA GTCTCCTCAT 
TCCTCTAACC CAAGCAGCCT ACCCAGCTCT CCTCCAACAC ACAACCATAA TTCTGTTCCA 
TTCTCCAATT TTGGACCCAT TGGGACTCCA GATAACAGGG ATAGAAGGAC TGCAGATCGG 
TGGAAAACTG ATAAGCCAGC CATGGGTGGG TTTGGCATTG ATTATCTCTC AGCAACGTCA 
TCCTCTGAGA GCAGTTGGCA TCAGGCCAGC ACTCCGAGTG GCACCTGGAC AGGCCATGGC 
CCTTCCATGG AGGATTCCTC TGCTGTCCTC ATGGAAAGCC TAAAGTCTAT CTGGTCCAGT 
TCCATGATGC ATCCTGGACC TTCTGCTCTG GAGCAGCTGT TAATGCAGCA GAAGCAGAAA 
CAGCAACGGG GACAAGGCAC CATGAACCCT CCACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016858458.1
CDS	1318..3876
Translation	MSFLGILCKCPLQNESQEESYNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPHEEDLSSISATPLPEEFELQGFLALRPSFRNLDFSKGHQGITGDKEGQQRRIRQQRLISIGKWIADNQPRLIQCENEVGKLLFITEIPELILEDPSEAKENLILQETSVIESLAADGSPGLKSVLSTSRNLSNNCDTGEKPVVTFKENIKTREVNRDQGRSFPPKEVRRDYSKGITVTKNDGKKDNNKRKTETKKCTLEKLQETGKQNVAVQVKSQTELRKTPVSEARKTPVTQTPTQASNSQFIPIHHPGAFPPLPSRPGFPPPTYVIPPPVAFSMGSGYTFPAGVSVPGTFLQPTAHSPAGNQVQAGKQSHIPYSQQRPSGPGPMNQGPQQSQPPSQQPLTSLPAQPTAQSTSQLQVQALTQQQQSPTKAVPALGKSPPHHSGFQQYQQADASKQLWNPPQVQGPLGKIMPVKQPYYLQTQDPIKLFEPSLQPPVMQQQPLEKKMKPFPMEPYNHNPSEVKVPEFYWDSSYSMADNRSVMAQQANIDRRGKRSPGVFRPEQDPVPRMPFEDPKSSPLLPPDLLKSLAALEEEEELIFSNPPDLYPALLGPLASLPGRSLFKSLLEKPSELMSHSSSFLSLTGFSLNQERYPNNSMFNEVYGKNLTSSSKAELSPSMAPQETSLYSLFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSVPFSNFGPIGTPDNRDRRTADRWKTDKPAMGGFGIDYLSATSSSESSWHQASTPSGTWTGHGPSMEDSSAVLMESLKSIWSSSMMHPGPSALEQLLMQQKQKQQRGQGTMNPPH

Target ORF information:

RefSeq Version	XM_017002969.1
Organism	Homo sapiens(human)
Definition	Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017002969.1

ORF Insert Sequence:

ATGTCTTTTC TTGGCATCCT GTGCAAGTGT CCTCTACAGA ATGAGTCTCA GGAGGAGTCC 
TACAATGCCT ATCCTCTTCC AGCAGTCAAG GTCTCCATGG ACTGGCTAAG ACTCAGACCC 
AGGGTCTTTC AGGAGGCAGT GGTGGATGAA AGACAGTACA TTTGGCCCTG GTTGATTTCT 
CTTCTGAATA GTTTCCATCC CCATGAAGAG GACCTCTCAA GTATTAGTGC GACACCACTT 
CCAGAGGAGT TTGAATTACA AGGATTTTTG GCATTGAGAC CTTCTTTCAG GAACTTGGAT 
TTTTCCAAAG GTCACCAGGG TATTACAGGG GACAAAGAAG GCCAGCAACG ACGAATACGA 
CAGCAACGCT TGATCTCTAT AGGCAAATGG ATTGCTGATA ATCAGCCAAG GCTGATTCAG 
TGTGAAAATG AGGTAGGGAA ATTGTTGTTT ATCACAGAAA TCCCAGAATT AATACTGGAA 
GACCCCAGTG AAGCCAAAGA GAACCTCATT CTGCAAGAAA CATCTGTGAT AGAGTCGCTG 
GCTGCAGATG GGAGCCCAGG GCTAAAATCA GTGCTATCTA CAAGCCGAAA TTTAAGCAAC 
AACTGTGACA CAGGAGAGAA GCCAGTGGTT ACCTTCAAAG AAAACATTAA GACACGAGAA 
GTGAACAGAG ACCAAGGAAG AAGTTTTCCT CCCAAAGAGG TGAGAAGGGA CTATAGCAAA 
GGAATAACTG TAACTAAGAA TGATGGAAAG AAGGACAACA ACAAGAGGAA AACTGAAACC 
AAGAAATGCA CCTTAGAAAA GTTACAGGAA ACAGGAAAGC AGAATGTGGC AGTGCAGGTA 
AAATCCCAGA CAGAACTAAG AAAGACTCCA GTGTCTGAAG CCAGAAAAAC ACCTGTAACT 
CAAACCCCAA CTCAAGCAAG TAACTCCCAG TTCATCCCCA TTCATCACCC TGGAGCCTTC 
CCTCCTCTTC CCAGCAGGCC AGGGTTTCCG CCCCCAACAT ATGTTATCCC CCCGCCTGTG 
GCATTTTCTA TGGGCTCAGG TTACACCTTC CCAGCTGGTG TTTCTGTCCC AGGAACCTTT 
CTTCAGCCTA CAGCTCACTC TCCAGCAGGA AACCAGGTGC AAGCTGGGAA ACAGTCCCAC 
ATTCCTTACA GCCAGCAACG GCCCTCTGGA CCAGGGCCAA TGAACCAGGG ACCTCAACAA 
TCACAGCCAC CTTCCCAGCA ACCCCTTACA TCTTTACCAG CTCAGCCAAC AGCACAGTCT 
ACAAGCCAGC TGCAGGTTCA AGCTCTAACT CAGCAACAAC AATCCCCTAC AAAAGCTGTG 
CCGGCTTTGG GGAAAAGCCC GCCTCACCAC TCTGGATTCC AGCAGTATCA ACAGGCAGAT 
GCCTCCAAAC AGCTGTGGAA TCCCCCTCAG GTTCAAGGCC CATTAGGGAA AATTATGCCT 
GTGAAACAGC CCTACTACCT TCAGACCCAA GACCCCATAA AACTGTTTGA GCCGTCATTG 
CAACCTCCTG TAATGCAGCA GCAGCCTCTA GAAAAAAAAA TGAAGCCTTT TCCCATGGAG 
CCATATAACC ATAATCCCTC AGAAGTCAAG GTCCCAGAAT TCTACTGGGA TTCTTCCTAC 
AGCATGGCTG ATAACAGATC TGTAATGGCA CAGCAAGCAA ACATAGACCG CAGGGGCAAA 
CGGTCACCAG GAGTCTTCCG TCCAGAGCAG GATCCTGTAC CCAGAATGCC GTTTGAGGAC 
CCCAAGAGCT CCCCTCTGCT TCCTCCGGAC CTGTTAAAGA GTCTGGCTGC CTTGGAGGAA 
GAGGAAGAGC TGATTTTTTC TAACCCTCCT GATCTTTACC CGGCTCTGCT GGGGCCTCTC 
GCCTCTCTTC CTGGACGAAG CCTTTTTAAA TCCTTATTGG AGAAGCCCTC AGAGCTCATG 
TCACATTCAT CCTCTTTCCT GTCCCTCACC GGATTCTCTC TCAATCAGGA AAGATACCCA 
AATAATAGTA TGTTCAATGA GGTATATGGG AAAAACCTGA CATCCAGCTC CAAAGCAGAA 
CTCAGTCCCT CAATGGCCCC CCAGGAAACA TCTCTGTATT CCCTTTTTGA AGGGACTCCG 
TGGTCTCCAT CACTTCCTGC CAGTTCAGAT CATTCAACAC CAGCCAGCCA GTCTCCTCAT 
TCCTCTAACC CAAGCAGCCT ACCCAGCTCT CCTCCAACAC ACAACCATAA TTCTGTTCCA 
TTCTCCAATT TTGGACCCAT TGGGACTCCA GATAACAGGG ATAGAAGGAC TGCAGATCGG 
TGGAAAACTG ATAAGCCAGC CATGGGTGGG TTTGGCATTG ATTATCTCTC AGCAACGTCA 
TCCTCTGAGA GCAGTTGGCA TCAGGCCAGC ACTCCGAGTG GCACCTGGAC AGGCCATGGC 
CCTTCCATGG AGGATTCCTC TGCTGTCCTC ATGGAAAGCC TAAAGTCTAT CTGGTCCAGT 
TCCATGATGC ATCCTGGACC TTCTGCTCTG GAGCAGCTGT TAATGCAGCA GAAGCAGAAA 
CAGCAACGGG GACAAGGCAC CATGAACCCT CCACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG7 ( XM_017002970.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016858459.1 Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X13, mRNA.

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CloneID	OHu63457
Clone ID Related Accession (Same CDS sequence)	XM_017002969.1 , XM_017002970.1
Accession Version	XM_017002970.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2559bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein SMG7 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTTTC TTGGCATCCT GTGCAAGTGT CCTCTACAGA ATGAGTCTCA GGAGGAGTCC 
TACAATGCCT ATCCTCTTCC AGCAGTCAAG GTCTCCATGG ACTGGCTAAG ACTCAGACCC 
AGGGTCTTTC AGGAGGCAGT GGTGGATGAA AGACAGTACA TTTGGCCCTG GTTGATTTCT 
CTTCTGAATA GTTTCCATCC CCATGAAGAG GACCTCTCAA GTATTAGTGC GACACCACTT 
CCAGAGGAGT TTGAATTACA AGGATTTTTG GCATTGAGAC CTTCTTTCAG GAACTTGGAT 
TTTTCCAAAG GTCACCAGGG TATTACAGGG GACAAAGAAG GCCAGCAACG ACGAATACGA 
CAGCAACGCT TGATCTCTAT AGGCAAATGG ATTGCTGATA ATCAGCCAAG GCTGATTCAG 
TGTGAAAATG AGGTAGGGAA ATTGTTGTTT ATCACAGAAA TCCCAGAATT AATACTGGAA 
GACCCCAGTG AAGCCAAAGA GAACCTCATT CTGCAAGAAA CATCTGTGAT AGAGTCGCTG 
GCTGCAGATG GGAGCCCAGG GCTAAAATCA GTGCTATCTA CAAGCCGAAA TTTAAGCAAC 
AACTGTGACA CAGGAGAGAA GCCAGTGGTT ACCTTCAAAG AAAACATTAA GACACGAGAA 
GTGAACAGAG ACCAAGGAAG AAGTTTTCCT CCCAAAGAGG TGAGAAGGGA CTATAGCAAA 
GGAATAACTG TAACTAAGAA TGATGGAAAG AAGGACAACA ACAAGAGGAA AACTGAAACC 
AAGAAATGCA CCTTAGAAAA GTTACAGGAA ACAGGAAAGC AGAATGTGGC AGTGCAGGTA 
AAATCCCAGA CAGAACTAAG AAAGACTCCA GTGTCTGAAG CCAGAAAAAC ACCTGTAACT 
CAAACCCCAA CTCAAGCAAG TAACTCCCAG TTCATCCCCA TTCATCACCC TGGAGCCTTC 
CCTCCTCTTC CCAGCAGGCC AGGGTTTCCG CCCCCAACAT ATGTTATCCC CCCGCCTGTG 
GCATTTTCTA TGGGCTCAGG TTACACCTTC CCAGCTGGTG TTTCTGTCCC AGGAACCTTT 
CTTCAGCCTA CAGCTCACTC TCCAGCAGGA AACCAGGTGC AAGCTGGGAA ACAGTCCCAC 
ATTCCTTACA GCCAGCAACG GCCCTCTGGA CCAGGGCCAA TGAACCAGGG ACCTCAACAA 
TCACAGCCAC CTTCCCAGCA ACCCCTTACA TCTTTACCAG CTCAGCCAAC AGCACAGTCT 
ACAAGCCAGC TGCAGGTTCA AGCTCTAACT CAGCAACAAC AATCCCCTAC AAAAGCTGTG 
CCGGCTTTGG GGAAAAGCCC GCCTCACCAC TCTGGATTCC AGCAGTATCA ACAGGCAGAT 
GCCTCCAAAC AGCTGTGGAA TCCCCCTCAG GTTCAAGGCC CATTAGGGAA AATTATGCCT 
GTGAAACAGC CCTACTACCT TCAGACCCAA GACCCCATAA AACTGTTTGA GCCGTCATTG 
CAACCTCCTG TAATGCAGCA GCAGCCTCTA GAAAAAAAAA TGAAGCCTTT TCCCATGGAG 
CCATATAACC ATAATCCCTC AGAAGTCAAG GTCCCAGAAT TCTACTGGGA TTCTTCCTAC 
AGCATGGCTG ATAACAGATC TGTAATGGCA CAGCAAGCAA ACATAGACCG CAGGGGCAAA 
CGGTCACCAG GAGTCTTCCG TCCAGAGCAG GATCCTGTAC CCAGAATGCC GTTTGAGGAC 
CCCAAGAGCT CCCCTCTGCT TCCTCCGGAC CTGTTAAAGA GTCTGGCTGC CTTGGAGGAA 
GAGGAAGAGC TGATTTTTTC TAACCCTCCT GATCTTTACC CGGCTCTGCT GGGGCCTCTC 
GCCTCTCTTC CTGGACGAAG CCTTTTTAAA TCCTTATTGG AGAAGCCCTC AGAGCTCATG 
TCACATTCAT CCTCTTTCCT GTCCCTCACC GGATTCTCTC TCAATCAGGA AAGATACCCA 
AATAATAGTA TGTTCAATGA GGTATATGGG AAAAACCTGA CATCCAGCTC CAAAGCAGAA 
CTCAGTCCCT CAATGGCCCC CCAGGAAACA TCTCTGTATT CCCTTTTTGA AGGGACTCCG 
TGGTCTCCAT CACTTCCTGC CAGTTCAGAT CATTCAACAC CAGCCAGCCA GTCTCCTCAT 
TCCTCTAACC CAAGCAGCCT ACCCAGCTCT CCTCCAACAC ACAACCATAA TTCTGTTCCA 
TTCTCCAATT TTGGACCCAT TGGGACTCCA GATAACAGGG ATAGAAGGAC TGCAGATCGG 
TGGAAAACTG ATAAGCCAGC CATGGGTGGG TTTGGCATTG ATTATCTCTC AGCAACGTCA 
TCCTCTGAGA GCAGTTGGCA TCAGGCCAGC ACTCCGAGTG GCACCTGGAC AGGCCATGGC 
CCTTCCATGG AGGATTCCTC TGCTGTCCTC ATGGAAAGCC TAAAGTCTAT CTGGTCCAGT 
TCCATGATGC ATCCTGGACC TTCTGCTCTG GAGCAGCTGT TAATGCAGCA GAAGCAGAAA 
CAGCAACGGG GACAAGGCAC CATGAACCCT CCACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016858459.1
CDS	1242..3800
Translation	MSFLGILCKCPLQNESQEESYNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPHEEDLSSISATPLPEEFELQGFLALRPSFRNLDFSKGHQGITGDKEGQQRRIRQQRLISIGKWIADNQPRLIQCENEVGKLLFITEIPELILEDPSEAKENLILQETSVIESLAADGSPGLKSVLSTSRNLSNNCDTGEKPVVTFKENIKTREVNRDQGRSFPPKEVRRDYSKGITVTKNDGKKDNNKRKTETKKCTLEKLQETGKQNVAVQVKSQTELRKTPVSEARKTPVTQTPTQASNSQFIPIHHPGAFPPLPSRPGFPPPTYVIPPPVAFSMGSGYTFPAGVSVPGTFLQPTAHSPAGNQVQAGKQSHIPYSQQRPSGPGPMNQGPQQSQPPSQQPLTSLPAQPTAQSTSQLQVQALTQQQQSPTKAVPALGKSPPHHSGFQQYQQADASKQLWNPPQVQGPLGKIMPVKQPYYLQTQDPIKLFEPSLQPPVMQQQPLEKKMKPFPMEPYNHNPSEVKVPEFYWDSSYSMADNRSVMAQQANIDRRGKRSPGVFRPEQDPVPRMPFEDPKSSPLLPPDLLKSLAALEEEEELIFSNPPDLYPALLGPLASLPGRSLFKSLLEKPSELMSHSSSFLSLTGFSLNQERYPNNSMFNEVYGKNLTSSSKAELSPSMAPQETSLYSLFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSVPFSNFGPIGTPDNRDRRTADRWKTDKPAMGGFGIDYLSATSSSESSWHQASTPSGTWTGHGPSMEDSSAVLMESLKSIWSSSMMHPGPSALEQLLMQQKQKQQRGQGTMNPPH

Target ORF information:

RefSeq Version	XM_017002970.1
Organism	Homo sapiens(human)
Definition	Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017002970.1

ORF Insert Sequence:

ATGTCTTTTC TTGGCATCCT GTGCAAGTGT CCTCTACAGA ATGAGTCTCA GGAGGAGTCC 
TACAATGCCT ATCCTCTTCC AGCAGTCAAG GTCTCCATGG ACTGGCTAAG ACTCAGACCC 
AGGGTCTTTC AGGAGGCAGT GGTGGATGAA AGACAGTACA TTTGGCCCTG GTTGATTTCT 
CTTCTGAATA GTTTCCATCC CCATGAAGAG GACCTCTCAA GTATTAGTGC GACACCACTT 
CCAGAGGAGT TTGAATTACA AGGATTTTTG GCATTGAGAC CTTCTTTCAG GAACTTGGAT 
TTTTCCAAAG GTCACCAGGG TATTACAGGG GACAAAGAAG GCCAGCAACG ACGAATACGA 
CAGCAACGCT TGATCTCTAT AGGCAAATGG ATTGCTGATA ATCAGCCAAG GCTGATTCAG 
TGTGAAAATG AGGTAGGGAA ATTGTTGTTT ATCACAGAAA TCCCAGAATT AATACTGGAA 
GACCCCAGTG AAGCCAAAGA GAACCTCATT CTGCAAGAAA CATCTGTGAT AGAGTCGCTG 
GCTGCAGATG GGAGCCCAGG GCTAAAATCA GTGCTATCTA CAAGCCGAAA TTTAAGCAAC 
AACTGTGACA CAGGAGAGAA GCCAGTGGTT ACCTTCAAAG AAAACATTAA GACACGAGAA 
GTGAACAGAG ACCAAGGAAG AAGTTTTCCT CCCAAAGAGG TGAGAAGGGA CTATAGCAAA 
GGAATAACTG TAACTAAGAA TGATGGAAAG AAGGACAACA ACAAGAGGAA AACTGAAACC 
AAGAAATGCA CCTTAGAAAA GTTACAGGAA ACAGGAAAGC AGAATGTGGC AGTGCAGGTA 
AAATCCCAGA CAGAACTAAG AAAGACTCCA GTGTCTGAAG CCAGAAAAAC ACCTGTAACT 
CAAACCCCAA CTCAAGCAAG TAACTCCCAG TTCATCCCCA TTCATCACCC TGGAGCCTTC 
CCTCCTCTTC CCAGCAGGCC AGGGTTTCCG CCCCCAACAT ATGTTATCCC CCCGCCTGTG 
GCATTTTCTA TGGGCTCAGG TTACACCTTC CCAGCTGGTG TTTCTGTCCC AGGAACCTTT 
CTTCAGCCTA CAGCTCACTC TCCAGCAGGA AACCAGGTGC AAGCTGGGAA ACAGTCCCAC 
ATTCCTTACA GCCAGCAACG GCCCTCTGGA CCAGGGCCAA TGAACCAGGG ACCTCAACAA 
TCACAGCCAC CTTCCCAGCA ACCCCTTACA TCTTTACCAG CTCAGCCAAC AGCACAGTCT 
ACAAGCCAGC TGCAGGTTCA AGCTCTAACT CAGCAACAAC AATCCCCTAC AAAAGCTGTG 
CCGGCTTTGG GGAAAAGCCC GCCTCACCAC TCTGGATTCC AGCAGTATCA ACAGGCAGAT 
GCCTCCAAAC AGCTGTGGAA TCCCCCTCAG GTTCAAGGCC CATTAGGGAA AATTATGCCT 
GTGAAACAGC CCTACTACCT TCAGACCCAA GACCCCATAA AACTGTTTGA GCCGTCATTG 
CAACCTCCTG TAATGCAGCA GCAGCCTCTA GAAAAAAAAA TGAAGCCTTT TCCCATGGAG 
CCATATAACC ATAATCCCTC AGAAGTCAAG GTCCCAGAAT TCTACTGGGA TTCTTCCTAC 
AGCATGGCTG ATAACAGATC TGTAATGGCA CAGCAAGCAA ACATAGACCG CAGGGGCAAA 
CGGTCACCAG GAGTCTTCCG TCCAGAGCAG GATCCTGTAC CCAGAATGCC GTTTGAGGAC 
CCCAAGAGCT CCCCTCTGCT TCCTCCGGAC CTGTTAAAGA GTCTGGCTGC CTTGGAGGAA 
GAGGAAGAGC TGATTTTTTC TAACCCTCCT GATCTTTACC CGGCTCTGCT GGGGCCTCTC 
GCCTCTCTTC CTGGACGAAG CCTTTTTAAA TCCTTATTGG AGAAGCCCTC AGAGCTCATG 
TCACATTCAT CCTCTTTCCT GTCCCTCACC GGATTCTCTC TCAATCAGGA AAGATACCCA 
AATAATAGTA TGTTCAATGA GGTATATGGG AAAAACCTGA CATCCAGCTC CAAAGCAGAA 
CTCAGTCCCT CAATGGCCCC CCAGGAAACA TCTCTGTATT CCCTTTTTGA AGGGACTCCG 
TGGTCTCCAT CACTTCCTGC CAGTTCAGAT CATTCAACAC CAGCCAGCCA GTCTCCTCAT 
TCCTCTAACC CAAGCAGCCT ACCCAGCTCT CCTCCAACAC ACAACCATAA TTCTGTTCCA 
TTCTCCAATT TTGGACCCAT TGGGACTCCA GATAACAGGG ATAGAAGGAC TGCAGATCGG 
TGGAAAACTG ATAAGCCAGC CATGGGTGGG TTTGGCATTG ATTATCTCTC AGCAACGTCA 
TCCTCTGAGA GCAGTTGGCA TCAGGCCAGC ACTCCGAGTG GCACCTGGAC AGGCCATGGC 
CCTTCCATGG AGGATTCCTC TGCTGTCCTC ATGGAAAGCC TAAAGTCTAT CTGGTCCAGT 
TCCATGATGC ATCCTGGACC TTCTGCTCTG GAGCAGCTGT TAATGCAGCA GAAGCAGAAA 
CAGCAACGGG GACAAGGCAC CATGAACCCT CCACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG7 ( NM_001350222.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001337151.1 Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 10, mRNA.

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CloneID	OHu102343
Clone ID Related Accession (Same CDS sequence)	NM_001350222.2 , NM_001350222.1
Accession Version	NM_001350222.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2421bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-29
Organism	Homo sapiens(human)
Product	protein SMG7 isoform 9
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB223038.1, DA773831.1, AB085674.1, BC036381.1, AK299178.1, DA629659.1 and CN479993.1. On Jun 2, 2019 this sequence version replaced NM_001350222.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7346977.2850010.1, SRR1660807.228673.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTTTTC TTGGCATCCT GTGCAAGTGT CCTCTACAGA ATGAGTCTCA GGAGGAGTCC 
TACAATGCCT ATCCTCTTCC AGCAGTCAAG GTCTCCATGG ACTGGCTAAG ACTCAGACCC 
AGGGTCTTTC AGGAGGCAGT GGTGGATGAA AGACAGTACA TTTGGCCCTG GTTGATTTCT 
CTTCTGAATA GTTTCCATCC CCATGAAGAG GACCTCTCAA GTATTAGTGC GACACCACTT 
CCAGAGGAGT TTGAATTACA AGGATTTTTG GCATTGAGAC CTTCTTTCAG GAACTTGGAT 
TTTTCCAAAG GTCACCAGGG TATTACAGGG GACAAAGAAG GCCAGCAACG ACGAATACGA 
CAGCAACGCT TGATCTCTAT AGGCAAATGG ATTGCTGATA ATCAGCCAAG GCTGATTCAG 
TGTGAAAATG AGGTAGGGAA ATTGTTGTTT ATCACAGAAA TCCCAGAATT AATACTGGAA 
GACCCCAGTG AAGCCAAAGA GAACCTCATT CTGCAAGAAA CATCTGTGAT AGAGTCGCTG 
GCTGCAGATG GGAGCCCAGG GCTAAAATCA GTGCTATCTA CAAGCCGAAA TTTAAGCAAC 
AACTGTGACA CAGGAGAGAA GCCAGTGGTT ACCTTCAAAG AAAACATTAA GACACGAGAA 
GTGAACAGAG ACCAAGGAAG AAGTTTTCCT CCCAAAGAGG TAAAATCCCA GACAGAACTA 
AGAAAGACTC CAGTGTCTGA AGCCAGAAAA ACACCTGTAA CTCAAACCCC AACTCAAGCA 
AGTAACTCCC AGTTCATCCC CATTCATCAC CCTGGAGCCT TCCCTCCTCT TCCCAGCAGG 
CCAGGGTTTC CGCCCCCAAC ATATGTTATC CCCCCGCCTG TGGCATTTTC TATGGGCTCA 
GGTTACACCT TCCCAGCTGG TGTTTCTGTC CCAGGAACCT TTCTTCAGCC TACAGCTCAC 
TCTCCAGCAG GAAACCAGGT GCAAGCTGGG AAACAGTCCC ACATTCCTTA CAGCCAGCAA 
CGGCCCTCTG GACCAGGGCC AATGAACCAG GGACCTCAAC AATCACAGCC ACCTTCCCAG 
CAACCCCTTA CATCTTTACC AGCTCAGCCA ACAGCACAGT CTACAAGCCA GCTGCAGGTT 
CAAGCTCTAA CTCAGCAACA ACAATCCCCT ACAAAAGCTG TGCCGGCTTT GGGGAAAAGC 
CCGCCTCACC ACTCTGGATT CCAGCAGTAT CAACAGGCAG ATGCCTCCAA ACAGCTGTGG 
AATCCCCCTC AGGTTCAAGG CCCATTAGGG AAAATTATGC CTGTGAAACA GCCCTACTAC 
CTTCAGACCC AAGACCCCAT AAAACTGTTT GAGCCGTCAT TGCAACCTCC TGTAATGCAG 
CAGCAGCCTC TAGAAAAAAA AATGAAGCCT TTTCCCATGG AGCCATATAA CCATAATCCC 
TCAGAAGTCA AGGTCCCAGA ATTCTACTGG GATTCTTCCT ACAGCATGGC TGATAACAGA 
TCTGTAATGG CACAGCAAGC AAACATAGAC CGCAGGGGCA AACGGTCACC AGGAGTCTTC 
CGTCCAGAGC AGGATCCTGT ACCCAGAATG CCGTTTGAGG ACCCCAAGAG CTCCCCTCTG 
CTTCCTCCGG ACCTGTTAAA GAGTCTGGCT GCCTTGGAGG AAGAGGAAGA GCTGATTTTT 
TCTAACCCTC CTGATCTTTA CCCGGCTCTG CTGGGGCCTC TCGCCTCTCT TCCTGGACGA 
AGCCTTTTTA AATCCTTATT GGAGAAGCCC TCAGAGCTCA TGTCACATTC ATCCTCTTTC 
CTGTCCCTCA CCGGATTCTC TCTCAATCAG GAAAGATACC CAAATAATAG TATGTTCAAT 
GAGGTATATG GGAAAAACCT GACATCCAGC TCCAAAGCAG AACTCAGTCC CTCAATGGCC 
CCCCAGGAAA CATCTCTGTA TTCCCTTTTT GAAGGGACTC CGTGGTCTCC ATCACTTCCT 
GCCAGTTCAG ATCATTCAAC ACCAGCCAGC CAGTCTCCTC ATTCCTCTAA CCCAAGCAGC 
CTACCCAGCT CTCCTCCAAC ACACAACCAT AATTCTGTTC CATTCTCCAA TTTTGGACCC 
ATTGGGACTC CAGATAACAG GGATAGAAGG ACTGCAGATC GGTGGAAAAC TGATAAGCCA 
GCCATGGGTG GGTTTGGCAT TGATTATCTC TCAGCAACGT CATCCTCTGA GAGCAGTTGG 
CATCAGGCCA GCACTCCGAG TGGCACCTGG ACAGGCCATG GCCCTTCCAT GGAGGATTCC 
TCTGCTGTCC TCATGGAAAG CCTAAAGTCT ATCTGGTCCA GTTCCATGAT GCATCCTGGA 
CCTTCTGCTC TGGAGCAGCT GTTAATGCAG CAGAAGCAGA AACAGCAACG GGGACAAGGC 
ACCATGAACC CTCCACACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001337151.1
CDS	1190..3610
Translation	MSFLGILCKCPLQNESQEESYNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPHEEDLSSISATPLPEEFELQGFLALRPSFRNLDFSKGHQGITGDKEGQQRRIRQQRLISIGKWIADNQPRLIQCENEVGKLLFITEIPELILEDPSEAKENLILQETSVIESLAADGSPGLKSVLSTSRNLSNNCDTGEKPVVTFKENIKTREVNRDQGRSFPPKEVKSQTELRKTPVSEARKTPVTQTPTQASNSQFIPIHHPGAFPPLPSRPGFPPPTYVIPPPVAFSMGSGYTFPAGVSVPGTFLQPTAHSPAGNQVQAGKQSHIPYSQQRPSGPGPMNQGPQQSQPPSQQPLTSLPAQPTAQSTSQLQVQALTQQQQSPTKAVPALGKSPPHHSGFQQYQQADASKQLWNPPQVQGPLGKIMPVKQPYYLQTQDPIKLFEPSLQPPVMQQQPLEKKMKPFPMEPYNHNPSEVKVPEFYWDSSYSMADNRSVMAQQANIDRRGKRSPGVFRPEQDPVPRMPFEDPKSSPLLPPDLLKSLAALEEEEELIFSNPPDLYPALLGPLASLPGRSLFKSLLEKPSELMSHSSSFLSLTGFSLNQERYPNNSMFNEVYGKNLTSSSKAELSPSMAPQETSLYSLFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSVPFSNFGPIGTPDNRDRRTADRWKTDKPAMGGFGIDYLSATSSSESSWHQASTPSGTWTGHGPSMEDSSAVLMESLKSIWSSSMMHPGPSALEQLLMQQKQKQQRGQGTMNPPH

Target ORF information:

RefSeq Version	NM_001350222.2
Organism	Homo sapiens(human)
Definition	Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001350222.2

ORF Insert Sequence:

ATGTCTTTTC TTGGCATCCT GTGCAAGTGT CCTCTACAGA ATGAGTCTCA GGAGGAGTCC 
TACAATGCCT ATCCTCTTCC AGCAGTCAAG GTCTCCATGG ACTGGCTAAG ACTCAGACCC 
AGGGTCTTTC AGGAGGCAGT GGTGGATGAA AGACAGTACA TTTGGCCCTG GTTGATTTCT 
CTTCTGAATA GTTTCCATCC CCATGAAGAG GACCTCTCAA GTATTAGTGC GACACCACTT 
CCAGAGGAGT TTGAATTACA AGGATTTTTG GCATTGAGAC CTTCTTTCAG GAACTTGGAT 
TTTTCCAAAG GTCACCAGGG TATTACAGGG GACAAAGAAG GCCAGCAACG ACGAATACGA 
CAGCAACGCT TGATCTCTAT AGGCAAATGG ATTGCTGATA ATCAGCCAAG GCTGATTCAG 
TGTGAAAATG AGGTAGGGAA ATTGTTGTTT ATCACAGAAA TCCCAGAATT AATACTGGAA 
GACCCCAGTG AAGCCAAAGA GAACCTCATT CTGCAAGAAA CATCTGTGAT AGAGTCGCTG 
GCTGCAGATG GGAGCCCAGG GCTAAAATCA GTGCTATCTA CAAGCCGAAA TTTAAGCAAC 
AACTGTGACA CAGGAGAGAA GCCAGTGGTT ACCTTCAAAG AAAACATTAA GACACGAGAA 
GTGAACAGAG ACCAAGGAAG AAGTTTTCCT CCCAAAGAGG TAAAATCCCA GACAGAACTA 
AGAAAGACTC CAGTGTCTGA AGCCAGAAAA ACACCTGTAA CTCAAACCCC AACTCAAGCA 
AGTAACTCCC AGTTCATCCC CATTCATCAC CCTGGAGCCT TCCCTCCTCT TCCCAGCAGG 
CCAGGGTTTC CGCCCCCAAC ATATGTTATC CCCCCGCCTG TGGCATTTTC TATGGGCTCA 
GGTTACACCT TCCCAGCTGG TGTTTCTGTC CCAGGAACCT TTCTTCAGCC TACAGCTCAC 
TCTCCAGCAG GAAACCAGGT GCAAGCTGGG AAACAGTCCC ACATTCCTTA CAGCCAGCAA 
CGGCCCTCTG GACCAGGGCC AATGAACCAG GGACCTCAAC AATCACAGCC ACCTTCCCAG 
CAACCCCTTA CATCTTTACC AGCTCAGCCA ACAGCACAGT CTACAAGCCA GCTGCAGGTT 
CAAGCTCTAA CTCAGCAACA ACAATCCCCT ACAAAAGCTG TGCCGGCTTT GGGGAAAAGC 
CCGCCTCACC ACTCTGGATT CCAGCAGTAT CAACAGGCAG ATGCCTCCAA ACAGCTGTGG 
AATCCCCCTC AGGTTCAAGG CCCATTAGGG AAAATTATGC CTGTGAAACA GCCCTACTAC 
CTTCAGACCC AAGACCCCAT AAAACTGTTT GAGCCGTCAT TGCAACCTCC TGTAATGCAG 
CAGCAGCCTC TAGAAAAAAA AATGAAGCCT TTTCCCATGG AGCCATATAA CCATAATCCC 
TCAGAAGTCA AGGTCCCAGA ATTCTACTGG GATTCTTCCT ACAGCATGGC TGATAACAGA 
TCTGTAATGG CACAGCAAGC AAACATAGAC CGCAGGGGCA AACGGTCACC AGGAGTCTTC 
CGTCCAGAGC AGGATCCTGT ACCCAGAATG CCGTTTGAGG ACCCCAAGAG CTCCCCTCTG 
CTTCCTCCGG ACCTGTTAAA GAGTCTGGCT GCCTTGGAGG AAGAGGAAGA GCTGATTTTT 
TCTAACCCTC CTGATCTTTA CCCGGCTCTG CTGGGGCCTC TCGCCTCTCT TCCTGGACGA 
AGCCTTTTTA AATCCTTATT GGAGAAGCCC TCAGAGCTCA TGTCACATTC ATCCTCTTTC 
CTGTCCCTCA CCGGATTCTC TCTCAATCAG GAAAGATACC CAAATAATAG TATGTTCAAT 
GAGGTATATG GGAAAAACCT GACATCCAGC TCCAAAGCAG AACTCAGTCC CTCAATGGCC 
CCCCAGGAAA CATCTCTGTA TTCCCTTTTT GAAGGGACTC CGTGGTCTCC ATCACTTCCT 
GCCAGTTCAG ATCATTCAAC ACCAGCCAGC CAGTCTCCTC ATTCCTCTAA CCCAAGCAGC 
CTACCCAGCT CTCCTCCAAC ACACAACCAT AATTCTGTTC CATTCTCCAA TTTTGGACCC 
ATTGGGACTC CAGATAACAG GGATAGAAGG ACTGCAGATC GGTGGAAAAC TGATAAGCCA 
GCCATGGGTG GGTTTGGCAT TGATTATCTC TCAGCAACGT CATCCTCTGA GAGCAGTTGG 
CATCAGGCCA GCACTCCGAG TGGCACCTGG ACAGGCCATG GCCCTTCCAT GGAGGATTCC 
TCTGCTGTCC TCATGGAAAG CCTAAAGTCT ATCTGGTCCA GTTCCATGAT GCATCCTGGA 
CCTTCTGCTC TGGAGCAGCT GTTAATGCAG CAGAAGCAGA AACAGCAACG GGGACAAGGC 
ACCATGAACC CTCCACACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG7 ( NM_001350222.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001337151.1 Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 10, mRNA.

Price & Availability↑

CloneID	OHu102343
Clone ID Related Accession (Same CDS sequence)	NM_001350222.2 , NM_001350222.1
Accession Version	NM_001350222.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2421bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-27
Organism	Homo sapiens(human)
Product	protein SMG7 isoform 9
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB223038.1, DA773831.1, AB085674.1, BC036381.1, AK299178.1, DA629659.1 and CN479993.1. On Apr 10, 2017 this sequence version replaced XM_017002971.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.228673.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTTTTC TTGGCATCCT GTGCAAGTGT CCTCTACAGA ATGAGTCTCA GGAGGAGTCC 
TACAATGCCT ATCCTCTTCC AGCAGTCAAG GTCTCCATGG ACTGGCTAAG ACTCAGACCC 
AGGGTCTTTC AGGAGGCAGT GGTGGATGAA AGACAGTACA TTTGGCCCTG GTTGATTTCT 
CTTCTGAATA GTTTCCATCC CCATGAAGAG GACCTCTCAA GTATTAGTGC GACACCACTT 
CCAGAGGAGT TTGAATTACA AGGATTTTTG GCATTGAGAC CTTCTTTCAG GAACTTGGAT 
TTTTCCAAAG GTCACCAGGG TATTACAGGG GACAAAGAAG GCCAGCAACG ACGAATACGA 
CAGCAACGCT TGATCTCTAT AGGCAAATGG ATTGCTGATA ATCAGCCAAG GCTGATTCAG 
TGTGAAAATG AGGTAGGGAA ATTGTTGTTT ATCACAGAAA TCCCAGAATT AATACTGGAA 
GACCCCAGTG AAGCCAAAGA GAACCTCATT CTGCAAGAAA CATCTGTGAT AGAGTCGCTG 
GCTGCAGATG GGAGCCCAGG GCTAAAATCA GTGCTATCTA CAAGCCGAAA TTTAAGCAAC 
AACTGTGACA CAGGAGAGAA GCCAGTGGTT ACCTTCAAAG AAAACATTAA GACACGAGAA 
GTGAACAGAG ACCAAGGAAG AAGTTTTCCT CCCAAAGAGG TAAAATCCCA GACAGAACTA 
AGAAAGACTC CAGTGTCTGA AGCCAGAAAA ACACCTGTAA CTCAAACCCC AACTCAAGCA 
AGTAACTCCC AGTTCATCCC CATTCATCAC CCTGGAGCCT TCCCTCCTCT TCCCAGCAGG 
CCAGGGTTTC CGCCCCCAAC ATATGTTATC CCCCCGCCTG TGGCATTTTC TATGGGCTCA 
GGTTACACCT TCCCAGCTGG TGTTTCTGTC CCAGGAACCT TTCTTCAGCC TACAGCTCAC 
TCTCCAGCAG GAAACCAGGT GCAAGCTGGG AAACAGTCCC ACATTCCTTA CAGCCAGCAA 
CGGCCCTCTG GACCAGGGCC AATGAACCAG GGACCTCAAC AATCACAGCC ACCTTCCCAG 
CAACCCCTTA CATCTTTACC AGCTCAGCCA ACAGCACAGT CTACAAGCCA GCTGCAGGTT 
CAAGCTCTAA CTCAGCAACA ACAATCCCCT ACAAAAGCTG TGCCGGCTTT GGGGAAAAGC 
CCGCCTCACC ACTCTGGATT CCAGCAGTAT CAACAGGCAG ATGCCTCCAA ACAGCTGTGG 
AATCCCCCTC AGGTTCAAGG CCCATTAGGG AAAATTATGC CTGTGAAACA GCCCTACTAC 
CTTCAGACCC AAGACCCCAT AAAACTGTTT GAGCCGTCAT TGCAACCTCC TGTAATGCAG 
CAGCAGCCTC TAGAAAAAAA AATGAAGCCT TTTCCCATGG AGCCATATAA CCATAATCCC 
TCAGAAGTCA AGGTCCCAGA ATTCTACTGG GATTCTTCCT ACAGCATGGC TGATAACAGA 
TCTGTAATGG CACAGCAAGC AAACATAGAC CGCAGGGGCA AACGGTCACC AGGAGTCTTC 
CGTCCAGAGC AGGATCCTGT ACCCAGAATG CCGTTTGAGG ACCCCAAGAG CTCCCCTCTG 
CTTCCTCCGG ACCTGTTAAA GAGTCTGGCT GCCTTGGAGG AAGAGGAAGA GCTGATTTTT 
TCTAACCCTC CTGATCTTTA CCCGGCTCTG CTGGGGCCTC TCGCCTCTCT TCCTGGACGA 
AGCCTTTTTA AATCCTTATT GGAGAAGCCC TCAGAGCTCA TGTCACATTC ATCCTCTTTC 
CTGTCCCTCA CCGGATTCTC TCTCAATCAG GAAAGATACC CAAATAATAG TATGTTCAAT 
GAGGTATATG GGAAAAACCT GACATCCAGC TCCAAAGCAG AACTCAGTCC CTCAATGGCC 
CCCCAGGAAA CATCTCTGTA TTCCCTTTTT GAAGGGACTC CGTGGTCTCC ATCACTTCCT 
GCCAGTTCAG ATCATTCAAC ACCAGCCAGC CAGTCTCCTC ATTCCTCTAA CCCAAGCAGC 
CTACCCAGCT CTCCTCCAAC ACACAACCAT AATTCTGTTC CATTCTCCAA TTTTGGACCC 
ATTGGGACTC CAGATAACAG GGATAGAAGG ACTGCAGATC GGTGGAAAAC TGATAAGCCA 
GCCATGGGTG GGTTTGGCAT TGATTATCTC TCAGCAACGT CATCCTCTGA GAGCAGTTGG 
CATCAGGCCA GCACTCCGAG TGGCACCTGG ACAGGCCATG GCCCTTCCAT GGAGGATTCC 
TCTGCTGTCC TCATGGAAAG CCTAAAGTCT ATCTGGTCCA GTTCCATGAT GCATCCTGGA 
CCTTCTGCTC TGGAGCAGCT GTTAATGCAG CAGAAGCAGA AACAGCAACG GGGACAAGGC 
ACCATGAACC CTCCACACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001337151.1
CDS	1318..3738
Translation	MSFLGILCKCPLQNESQEESYNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPHEEDLSSISATPLPEEFELQGFLALRPSFRNLDFSKGHQGITGDKEGQQRRIRQQRLISIGKWIADNQPRLIQCENEVGKLLFITEIPELILEDPSEAKENLILQETSVIESLAADGSPGLKSVLSTSRNLSNNCDTGEKPVVTFKENIKTREVNRDQGRSFPPKEVKSQTELRKTPVSEARKTPVTQTPTQASNSQFIPIHHPGAFPPLPSRPGFPPPTYVIPPPVAFSMGSGYTFPAGVSVPGTFLQPTAHSPAGNQVQAGKQSHIPYSQQRPSGPGPMNQGPQQSQPPSQQPLTSLPAQPTAQSTSQLQVQALTQQQQSPTKAVPALGKSPPHHSGFQQYQQADASKQLWNPPQVQGPLGKIMPVKQPYYLQTQDPIKLFEPSLQPPVMQQQPLEKKMKPFPMEPYNHNPSEVKVPEFYWDSSYSMADNRSVMAQQANIDRRGKRSPGVFRPEQDPVPRMPFEDPKSSPLLPPDLLKSLAALEEEEELIFSNPPDLYPALLGPLASLPGRSLFKSLLEKPSELMSHSSSFLSLTGFSLNQERYPNNSMFNEVYGKNLTSSSKAELSPSMAPQETSLYSLFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSVPFSNFGPIGTPDNRDRRTADRWKTDKPAMGGFGIDYLSATSSSESSWHQASTPSGTWTGHGPSMEDSSAVLMESLKSIWSSSMMHPGPSALEQLLMQQKQKQQRGQGTMNPPH

Target ORF information:

RefSeq Version	NM_001350222.1
Organism	Homo sapiens(human)
Definition	Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001350222.1

ORF Insert Sequence:

ATGTCTTTTC TTGGCATCCT GTGCAAGTGT CCTCTACAGA ATGAGTCTCA GGAGGAGTCC 
TACAATGCCT ATCCTCTTCC AGCAGTCAAG GTCTCCATGG ACTGGCTAAG ACTCAGACCC 
AGGGTCTTTC AGGAGGCAGT GGTGGATGAA AGACAGTACA TTTGGCCCTG GTTGATTTCT 
CTTCTGAATA GTTTCCATCC CCATGAAGAG GACCTCTCAA GTATTAGTGC GACACCACTT 
CCAGAGGAGT TTGAATTACA AGGATTTTTG GCATTGAGAC CTTCTTTCAG GAACTTGGAT 
TTTTCCAAAG GTCACCAGGG TATTACAGGG GACAAAGAAG GCCAGCAACG ACGAATACGA 
CAGCAACGCT TGATCTCTAT AGGCAAATGG ATTGCTGATA ATCAGCCAAG GCTGATTCAG 
TGTGAAAATG AGGTAGGGAA ATTGTTGTTT ATCACAGAAA TCCCAGAATT AATACTGGAA 
GACCCCAGTG AAGCCAAAGA GAACCTCATT CTGCAAGAAA CATCTGTGAT AGAGTCGCTG 
GCTGCAGATG GGAGCCCAGG GCTAAAATCA GTGCTATCTA CAAGCCGAAA TTTAAGCAAC 
AACTGTGACA CAGGAGAGAA GCCAGTGGTT ACCTTCAAAG AAAACATTAA GACACGAGAA 
GTGAACAGAG ACCAAGGAAG AAGTTTTCCT CCCAAAGAGG TAAAATCCCA GACAGAACTA 
AGAAAGACTC CAGTGTCTGA AGCCAGAAAA ACACCTGTAA CTCAAACCCC AACTCAAGCA 
AGTAACTCCC AGTTCATCCC CATTCATCAC CCTGGAGCCT TCCCTCCTCT TCCCAGCAGG 
CCAGGGTTTC CGCCCCCAAC ATATGTTATC CCCCCGCCTG TGGCATTTTC TATGGGCTCA 
GGTTACACCT TCCCAGCTGG TGTTTCTGTC CCAGGAACCT TTCTTCAGCC TACAGCTCAC 
TCTCCAGCAG GAAACCAGGT GCAAGCTGGG AAACAGTCCC ACATTCCTTA CAGCCAGCAA 
CGGCCCTCTG GACCAGGGCC AATGAACCAG GGACCTCAAC AATCACAGCC ACCTTCCCAG 
CAACCCCTTA CATCTTTACC AGCTCAGCCA ACAGCACAGT CTACAAGCCA GCTGCAGGTT 
CAAGCTCTAA CTCAGCAACA ACAATCCCCT ACAAAAGCTG TGCCGGCTTT GGGGAAAAGC 
CCGCCTCACC ACTCTGGATT CCAGCAGTAT CAACAGGCAG ATGCCTCCAA ACAGCTGTGG 
AATCCCCCTC AGGTTCAAGG CCCATTAGGG AAAATTATGC CTGTGAAACA GCCCTACTAC 
CTTCAGACCC AAGACCCCAT AAAACTGTTT GAGCCGTCAT TGCAACCTCC TGTAATGCAG 
CAGCAGCCTC TAGAAAAAAA AATGAAGCCT TTTCCCATGG AGCCATATAA CCATAATCCC 
TCAGAAGTCA AGGTCCCAGA ATTCTACTGG GATTCTTCCT ACAGCATGGC TGATAACAGA 
TCTGTAATGG CACAGCAAGC AAACATAGAC CGCAGGGGCA AACGGTCACC AGGAGTCTTC 
CGTCCAGAGC AGGATCCTGT ACCCAGAATG CCGTTTGAGG ACCCCAAGAG CTCCCCTCTG 
CTTCCTCCGG ACCTGTTAAA GAGTCTGGCT GCCTTGGAGG AAGAGGAAGA GCTGATTTTT 
TCTAACCCTC CTGATCTTTA CCCGGCTCTG CTGGGGCCTC TCGCCTCTCT TCCTGGACGA 
AGCCTTTTTA AATCCTTATT GGAGAAGCCC TCAGAGCTCA TGTCACATTC ATCCTCTTTC 
CTGTCCCTCA CCGGATTCTC TCTCAATCAG GAAAGATACC CAAATAATAG TATGTTCAAT 
GAGGTATATG GGAAAAACCT GACATCCAGC TCCAAAGCAG AACTCAGTCC CTCAATGGCC 
CCCCAGGAAA CATCTCTGTA TTCCCTTTTT GAAGGGACTC CGTGGTCTCC ATCACTTCCT 
GCCAGTTCAG ATCATTCAAC ACCAGCCAGC CAGTCTCCTC ATTCCTCTAA CCCAAGCAGC 
CTACCCAGCT CTCCTCCAAC ACACAACCAT AATTCTGTTC CATTCTCCAA TTTTGGACCC 
ATTGGGACTC CAGATAACAG GGATAGAAGG ACTGCAGATC GGTGGAAAAC TGATAAGCCA 
GCCATGGGTG GGTTTGGCAT TGATTATCTC TCAGCAACGT CATCCTCTGA GAGCAGTTGG 
CATCAGGCCA GCACTCCGAG TGGCACCTGG ACAGGCCATG GCCCTTCCAT GGAGGATTCC 
TCTGCTGTCC TCATGGAAAG CCTAAAGTCT ATCTGGTCCA GTTCCATGAT GCATCCTGGA 
CCTTCTGCTC TGGAGCAGCT GTTAATGCAG CAGAAGCAGA AACAGCAACG GGGACAAGGC 
ACCATGAACC CTCCACACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG7 ( NM_001375584.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001362513.1 Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 11, mRNA.

Price & Availability↑

CloneID	OHu34882
Clone ID Related Accession (Same CDS sequence)	XM_005245652.3 , NM_001375584.1
Accession Version	NM_001375584.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3564bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-28
Organism	Homo sapiens(human)
Product	protein SMG7 isoform 10
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL449223.7 and AL137800.12. On Oct 29, 2019 this sequence version replaced XM_005245652.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.221341.1, SRR1660807.81080.1 [ECO:0000332] ##Evidence-Data-END##

ATGAGCCTGC AGAGCGCGCA GTACCTCCGG CAGGCAGAAG TCCTGAAGGC TGACATGACA 
GATTCTAAGC TGGGTCCAGC TGAAGTCTGG ACATCCAGGC AGGCTCTGCA GGACCTGTAC 
CAGAAAATGC TAGTTACCGA TTTGGAATAC GCTTTAGACA AGAAAGTAGA ACAGGATCTC 
TGGAATCACG CCTTTAAGAA TCAGATCACA ACACTGCAAG GCCAGGCAAA GAATCGAGCA 
AATCCGAATC GGAGTGAAGT TCAGGCAAAC CTTTCTCTGT TCCTAGAGGC AGCTAGTGGC 
TTCTATACTC AGTTATTACA AGAACTGTGT ACAGTATTTA ATGTAGATTT ACCATGCCGT 
GTGAAGTCTT CCCAATTGGG AATTATCAGC AATAAACAGA CGCATACCAG CGCCATAGTG 
AAGCCACAGT CTAGCTCCTG TTCCTATATC TGCCAGCACT GCCTCGTCCA CCTTGGAGAC 
ATTGCTCGAT ACAGAAACCA GACCAGCCAG GCAGAGTCCT ACTATAGGCA TGCAGCTCAG 
CTTGTCCCCT CCAATGGTCA GCCTTATAAT CAGTTGGCTA TCTTAGCTTC TTCCAAAGGA 
GACCATCTGA CCACAATTTT CTACTACTGC AGAAGCATTG CTGTGAAGTT CCCTTTCCCA 
GCTGCCTCCA CTAATCTGCA AAAAGCACTT TCTAAAGCAC TGGAAAGCCG AGATGAGGTG 
AAAACCAAGT GGGGTGTTTC TGACTTCATC AAGGCCTTTA TTAAATTCCA CGGTCATGTG 
TACCTGAGTA AGAGCTTGGA AAAGTTGAGC CCTCTTCGAG AGAAATTGGA AGAACAGTTT 
AAGAGGCTGC TATTCCAAAA AGCTTTCAAC TCTCAGCAGT TAGTTCATGT CACTGTCATT 
AACCTGTTTC AACTTCATCA CCTTCGTGAC TTTAGCAATG AAACCGAGCA GCACACTTAT 
AGCCAAGATG AGCAGCTATG TTGGACACAG TTGCTGGCCC TCTTTATGTC TTTTCTTGGC 
ATCCTGTGCA AGTGTCCTCT ACAGAATGAG TCTCAGGAGG AGTCCTACAA TGCCTATCCT 
CTTCCAGCAG TCAAGGTCTC CATGGACTGG CTAAGACTCA GACCCAGGGT CTTTCAGGAG 
GCAGTGGTGG ATGAAAGACA GTACATTTGG CCCTGGTTGA TTTCTCTTCT GAATAGTTTC 
CATCCCCATG AAGAGGACCT CTCAAGTATT AGTGCGACAC CACTTCCAGA GGAGTTTGAA 
TTACAAGGAT TTTTGGCATT GAGACCTTCT TTCAGGAACT TGGATTTTTC CAAAGGTCAC 
CAGGGTATTA CAGGGGACAA AGAAGGCCAG CAACGACGAA TACGACAGCA ACGCTTGATC 
TCTATAGGCA AATGGATTGC TGATAATCAG CCAAGGCTGA TTCAGTGTGA AAATGAGGTA 
GGGAAATTGT TGTTTATCAC AGAAATCCCA GAATTAATAC TGGAAGACCC CAGTGAAGCC 
AAAGAGAACC TCATTCTGCA AGAAACATCT GTGATAGAGT CGCTGGCTGC AGATGGGAGC 
CCAGGGCTAA AATCAGTGCT ATCTACAAGC CGAAATTTAA GCAACAACTG TGACACAGGA 
GAGAAGCCAG TGGTTACCTT CAAAGAAAAC ATTAAGACAC GAGAAGTGAA CAGAGACCAA 
GGAAGAAGTT TTCCTCCCAA AGAGGTGAGA AGGGACTATA GCAAAGGAAT AACTGTAACT 
AAGAATGATG GAAAGAAGGA CAACAACAAG AGGAAAACTG AAACCAAGAA ATGCACCTTA 
GAAAAGTTAC AGGAAACAGG AAAGCAGAAT GTGGCAGTGC AGGTAAAATC CCAGACAGAA 
CTAAGAAAGA CTCCAGTGTC TGAAGCCAGA AAAACACCTG TAACTCAAAC CCCAACTCAA 
GCAAGTAACT CCCAGTTCAT CCCCATTCAT CACCCTGGAG CCTTCCCTCC TCTTCCCAGC 
AGGCCAGGGT TTCCGCCCCC AACATATGTT ATCCCCCCGC CTGTGGCATT TTCTATGGGC 
TCAGGTTACA CCTTCCCAGC TGGTGTTTCT GTCCCAGGAA CCTTTCTTCA GCCTACAGCT 
CACTCTCCAG CAGGAAACCA GGTGCAAGCT GGGAAACAGT CCCACATTCC TTACAGCCAG 
CAACGGCCCT CTGGACCAGG GCCAATGAAC CAGGGACCTC AACAATCACA GCCACCTTCC 
CAGCAACCCC TTACATCTTT ACCAGCTCAG CCAACAGCAC AGTCTACAAG CCAGCTGCAG 
GTTCAAGCTC TAACTCAGCA ACAACAATCC CCTACAAAAG CTGTGCCGGC TTTGGGGAAA 
AGCCCGCCTC ACCACTCTGG ATTCCAGCAG TATCAACAGG CAGATGCCTC CAAACAGCTG 
TGGAATCCCC CTCAGGTTCA AGGCCCATTA GGGAAAATTA TGCCTGTGAA ACAGCCCTAC 
TACCTTCAGA CCCAAGACCC CATAAAACTG TTTGAGCCGT CATTGCAACC TCCTGTAATG 
CAGCAGCAGC CTCTAGAAAA AAAAATGAAG CCTTTTCCCA TGGAGCCATA TAACCATAAT 
CCCTCAGAAG TCAAGGTCCC AGAATTCTAC TGGGATTCTT CCTACAGCAT GGCTGATAAC 
AGATCTGTAA TGGCACAGCA AGCAAACATA GACCGCAGGG GCAAACGGTC ACCAGGAGTC 
TTCCGTCCAG AGCAGGATCC TGTACCCAGA ATGCCGTTTG AGGACCCCAA GAGCTCCCCT 
CTGCTTCCTC CGGACCTGTT AAAGAGTCTG GCTGCCTTGG AGGAAGAGGA AGAGCTGATT 
TTTTCTAACC CTCCTGATCT TTACCCGGCT CTGCTGGGGC CTCTCGCCTC TCTTCCTGGA 
CGAAGCCTTT TTAAATCCTT ATTGGAGAAG CCCTCAGAGC TCATGTCACA TTCATCCTCT 
TTCCTGTCCC TCACCGGATT CTCTCTCAAT CAGGAAAGAT ACCCAAATAA TAGTATGTTC 
AATGAGGTAT ATGGGAAAAA CCTGACATCC AGCTCCAAAG CAGAACTCAG TCCCTCAATG 
GCCCCCCAGG AAACATCTCT GTATTCCCTT TTTGAAGGGA CTCCGTGGTC TCCATCACTT 
CCTGCCAGTT CAGATCATTC AACACCAGCC AGCCAGTCTC CTCATTCCTC TAACCCAAGC 
AGCCTACCCA GCTCTCCTCC AACACACAAC CATAATTCTG TTCCATTCTC CAATTTTGGA 
CCCATTGGGA CTCCAGATAA CAGGGATAGA AGGACTGCAG ATCGGTGGAA AACTGATAAG 
CCAGCCATGG GTGGGTTTGG CATTGATTAT CTCTCAGCAA CGTCATCCTC TGAGAGCAGT 
TGGCATCAGG CCAGCACTCC GAGTGGCACC TGGACAGGCC ATGGCCCTTC CATGGAGGAT 
TCCTCTGCTG TCCTCATGGA AAGCCTAAAG TCTATCTGGT CCAGTTCCAT GATGCATCCT 
GGACCTTCTG CTCTGGAGCA GCTGTTAATG CAGCAGAAGC AGAAACAGCA ACGGGGACAA 
GGCACCATGA ACCCTCCACA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001362513.1
CDS	123..3686
Translation	MSLQSAQYLRQAEVLKADMTDSKLGPAEVWTSRQALQDLYQKMLVTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRANPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQTHTSAIVKPQSSSCSYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNLQKALSKALESRDEVKTKWGVSDFIKAFIKFHGHVYLSKSLEKLSPLREKLEEQFKRLLFQKAFNSQQLVHVTVINLFQLHHLRDFSNETEQHTYSQDEQLCWTQLLALFMSFLGILCKCPLQNESQEESYNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPHEEDLSSISATPLPEEFELQGFLALRPSFRNLDFSKGHQGITGDKEGQQRRIRQQRLISIGKWIADNQPRLIQCENEVGKLLFITEIPELILEDPSEAKENLILQETSVIESLAADGSPGLKSVLSTSRNLSNNCDTGEKPVVTFKENIKTREVNRDQGRSFPPKEVRRDYSKGITVTKNDGKKDNNKRKTETKKCTLEKLQETGKQNVAVQVKSQTELRKTPVSEARKTPVTQTPTQASNSQFIPIHHPGAFPPLPSRPGFPPPTYVIPPPVAFSMGSGYTFPAGVSVPGTFLQPTAHSPAGNQVQAGKQSHIPYSQQRPSGPGPMNQGPQQSQPPSQQPLTSLPAQPTAQSTSQLQVQALTQQQQSPTKAVPALGKSPPHHSGFQQYQQADASKQLWNPPQVQGPLGKIMPVKQPYYLQTQDPIKLFEPSLQPPVMQQQPLEKKMKPFPMEPYNHNPSEVKVPEFYWDSSYSMADNRSVMAQQANIDRRGKRSPGVFRPEQDPVPRMPFEDPKSSPLLPPDLLKSLAALEEEEELIFSNPPDLYPALLGPLASLPGRSLFKSLLEKPSELMSHSSSFLSLTGFSLNQERYPNNSMFNEVYGKNLTSSSKAELSPSMAPQETSLYSLFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSVPFSNFGPIGTPDNRDRRTADRWKTDKPAMGGFGIDYLSATSSSESSWHQASTPSGTWTGHGPSMEDSSAVLMESLKSIWSSSMMHPGPSALEQLLMQQKQKQQRGQGTMNPPH

Target ORF information:

RefSeq Version	NM_001375584.1
Organism	Homo sapiens(human)
Definition	Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001375584.1

ORF Insert Sequence:

ATGAGCCTGC AGAGCGCGCA GTACCTCCGG CAGGCAGAAG TCCTGAAGGC TGACATGACA 
GATTCTAAGC TGGGTCCAGC TGAAGTCTGG ACATCCAGGC AGGCTCTGCA GGACCTGTAC 
CAGAAAATGC TAGTTACCGA TTTGGAATAC GCTTTAGACA AGAAAGTAGA ACAGGATCTC 
TGGAATCACG CCTTTAAGAA TCAGATCACA ACACTGCAAG GCCAGGCAAA GAATCGAGCA 
AATCCGAATC GGAGTGAAGT TCAGGCAAAC CTTTCTCTGT TCCTAGAGGC AGCTAGTGGC 
TTCTATACTC AGTTATTACA AGAACTGTGT ACAGTATTTA ATGTAGATTT ACCATGCCGT 
GTGAAGTCTT CCCAATTGGG AATTATCAGC AATAAACAGA CGCATACCAG CGCCATAGTG 
AAGCCACAGT CTAGCTCCTG TTCCTATATC TGCCAGCACT GCCTCGTCCA CCTTGGAGAC 
ATTGCTCGAT ACAGAAACCA GACCAGCCAG GCAGAGTCCT ACTATAGGCA TGCAGCTCAG 
CTTGTCCCCT CCAATGGTCA GCCTTATAAT CAGTTGGCTA TCTTAGCTTC TTCCAAAGGA 
GACCATCTGA CCACAATTTT CTACTACTGC AGAAGCATTG CTGTGAAGTT CCCTTTCCCA 
GCTGCCTCCA CTAATCTGCA AAAAGCACTT TCTAAAGCAC TGGAAAGCCG AGATGAGGTG 
AAAACCAAGT GGGGTGTTTC TGACTTCATC AAGGCCTTTA TTAAATTCCA CGGTCATGTG 
TACCTGAGTA AGAGCTTGGA AAAGTTGAGC CCTCTTCGAG AGAAATTGGA AGAACAGTTT 
AAGAGGCTGC TATTCCAAAA AGCTTTCAAC TCTCAGCAGT TAGTTCATGT CACTGTCATT 
AACCTGTTTC AACTTCATCA CCTTCGTGAC TTTAGCAATG AAACCGAGCA GCACACTTAT 
AGCCAAGATG AGCAGCTATG TTGGACACAG TTGCTGGCCC TCTTTATGTC TTTTCTTGGC 
ATCCTGTGCA AGTGTCCTCT ACAGAATGAG TCTCAGGAGG AGTCCTACAA TGCCTATCCT 
CTTCCAGCAG TCAAGGTCTC CATGGACTGG CTAAGACTCA GACCCAGGGT CTTTCAGGAG 
GCAGTGGTGG ATGAAAGACA GTACATTTGG CCCTGGTTGA TTTCTCTTCT GAATAGTTTC 
CATCCCCATG AAGAGGACCT CTCAAGTATT AGTGCGACAC CACTTCCAGA GGAGTTTGAA 
TTACAAGGAT TTTTGGCATT GAGACCTTCT TTCAGGAACT TGGATTTTTC CAAAGGTCAC 
CAGGGTATTA CAGGGGACAA AGAAGGCCAG CAACGACGAA TACGACAGCA ACGCTTGATC 
TCTATAGGCA AATGGATTGC TGATAATCAG CCAAGGCTGA TTCAGTGTGA AAATGAGGTA 
GGGAAATTGT TGTTTATCAC AGAAATCCCA GAATTAATAC TGGAAGACCC CAGTGAAGCC 
AAAGAGAACC TCATTCTGCA AGAAACATCT GTGATAGAGT CGCTGGCTGC AGATGGGAGC 
CCAGGGCTAA AATCAGTGCT ATCTACAAGC CGAAATTTAA GCAACAACTG TGACACAGGA 
GAGAAGCCAG TGGTTACCTT CAAAGAAAAC ATTAAGACAC GAGAAGTGAA CAGAGACCAA 
GGAAGAAGTT TTCCTCCCAA AGAGGTGAGA AGGGACTATA GCAAAGGAAT AACTGTAACT 
AAGAATGATG GAAAGAAGGA CAACAACAAG AGGAAAACTG AAACCAAGAA ATGCACCTTA 
GAAAAGTTAC AGGAAACAGG AAAGCAGAAT GTGGCAGTGC AGGTAAAATC CCAGACAGAA 
CTAAGAAAGA CTCCAGTGTC TGAAGCCAGA AAAACACCTG TAACTCAAAC CCCAACTCAA 
GCAAGTAACT CCCAGTTCAT CCCCATTCAT CACCCTGGAG CCTTCCCTCC TCTTCCCAGC 
AGGCCAGGGT TTCCGCCCCC AACATATGTT ATCCCCCCGC CTGTGGCATT TTCTATGGGC 
TCAGGTTACA CCTTCCCAGC TGGTGTTTCT GTCCCAGGAA CCTTTCTTCA GCCTACAGCT 
CACTCTCCAG CAGGAAACCA GGTGCAAGCT GGGAAACAGT CCCACATTCC TTACAGCCAG 
CAACGGCCCT CTGGACCAGG GCCAATGAAC CAGGGACCTC AACAATCACA GCCACCTTCC 
CAGCAACCCC TTACATCTTT ACCAGCTCAG CCAACAGCAC AGTCTACAAG CCAGCTGCAG 
GTTCAAGCTC TAACTCAGCA ACAACAATCC CCTACAAAAG CTGTGCCGGC TTTGGGGAAA 
AGCCCGCCTC ACCACTCTGG ATTCCAGCAG TATCAACAGG CAGATGCCTC CAAACAGCTG 
TGGAATCCCC CTCAGGTTCA AGGCCCATTA GGGAAAATTA TGCCTGTGAA ACAGCCCTAC 
TACCTTCAGA CCCAAGACCC CATAAAACTG TTTGAGCCGT CATTGCAACC TCCTGTAATG 
CAGCAGCAGC CTCTAGAAAA AAAAATGAAG CCTTTTCCCA TGGAGCCATA TAACCATAAT 
CCCTCAGAAG TCAAGGTCCC AGAATTCTAC TGGGATTCTT CCTACAGCAT GGCTGATAAC 
AGATCTGTAA TGGCACAGCA AGCAAACATA GACCGCAGGG GCAAACGGTC ACCAGGAGTC 
TTCCGTCCAG AGCAGGATCC TGTACCCAGA ATGCCGTTTG AGGACCCCAA GAGCTCCCCT 
CTGCTTCCTC CGGACCTGTT AAAGAGTCTG GCTGCCTTGG AGGAAGAGGA AGAGCTGATT 
TTTTCTAACC CTCCTGATCT TTACCCGGCT CTGCTGGGGC CTCTCGCCTC TCTTCCTGGA 
CGAAGCCTTT TTAAATCCTT ATTGGAGAAG CCCTCAGAGC TCATGTCACA TTCATCCTCT 
TTCCTGTCCC TCACCGGATT CTCTCTCAAT CAGGAAAGAT ACCCAAATAA TAGTATGTTC 
AATGAGGTAT ATGGGAAAAA CCTGACATCC AGCTCCAAAG CAGAACTCAG TCCCTCAATG 
GCCCCCCAGG AAACATCTCT GTATTCCCTT TTTGAAGGGA CTCCGTGGTC TCCATCACTT 
CCTGCCAGTT CAGATCATTC AACACCAGCC AGCCAGTCTC CTCATTCCTC TAACCCAAGC 
AGCCTACCCA GCTCTCCTCC AACACACAAC CATAATTCTG TTCCATTCTC CAATTTTGGA 
CCCATTGGGA CTCCAGATAA CAGGGATAGA AGGACTGCAG ATCGGTGGAA AACTGATAAG 
CCAGCCATGG GTGGGTTTGG CATTGATTAT CTCTCAGCAA CGTCATCCTC TGAGAGCAGT 
TGGCATCAGG CCAGCACTCC GAGTGGCACC TGGACAGGCC ATGGCCCTTC CATGGAGGAT 
TCCTCTGCTG TCCTCATGGA AAGCCTAAAG TCTATCTGGT CCAGTTCCAT GATGCATCCT 
GGACCTTCTG CTCTGGAGCA GCTGTTAATG CAGCAGAAGC AGAAACAGCA ACGGGGACAA 
GGCACCATGA ACCCTCCACA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG7 ( NM_001375585.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001362514.1 Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 12, mRNA.

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CloneID	OHu34886
Clone ID Related Accession (Same CDS sequence)	NM_001375585.1 , XM_006711678.3
Accession Version	NM_001375585.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3426bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-28
Organism	Homo sapiens(human)
Product	protein SMG7 isoform 11
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL449223.7 and AL137800.12. On Oct 29, 2019 this sequence version replaced XM_006711678.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7346977.2891567.1, SRR1803615.167462.1 [ECO:0000332] ##Evidence-Data-END##

ATGAGCCTGC AGAGCGCGCA GTACCTCCGG CAGGCAGAAG TCCTGAAGGC TGACATGACA 
GATTCTAAGC TGGGTCCAGC TGAAGTCTGG ACATCCAGGC AGGCTCTGCA GGACCTGTAC 
CAGAAAATGC TAGTTACCGA TTTGGAATAC GCTTTAGACA AGAAAGTAGA ACAGGATCTC 
TGGAATCACG CCTTTAAGAA TCAGATCACA ACACTGCAAG GCCAGGCAAA GAATCGAGCA 
AATCCGAATC GGAGTGAAGT TCAGGCAAAC CTTTCTCTGT TCCTAGAGGC AGCTAGTGGC 
TTCTATACTC AGTTATTACA AGAACTGTGT ACAGTATTTA ATGTAGATTT ACCATGCCGT 
GTGAAGTCTT CCCAATTGGG AATTATCAGC AATAAACAGA CGCATACCAG CGCCATAGTG 
AAGCCACAGT CTAGCTCCTG TTCCTATATC TGCCAGCACT GCCTCGTCCA CCTTGGAGAC 
ATTGCTCGAT ACAGAAACCA GACCAGCCAG GCAGAGTCCT ACTATAGGCA TGCAGCTCAG 
CTTGTCCCCT CCAATGGTCA GCCTTATAAT CAGTTGGCTA TCTTAGCTTC TTCCAAAGGA 
GACCATCTGA CCACAATTTT CTACTACTGC AGAAGCATTG CTGTGAAGTT CCCTTTCCCA 
GCTGCCTCCA CTAATCTGCA AAAAGCACTT TCTAAAGCAC TGGAAAGCCG AGATGAGGTG 
AAAACCAAGT GGGGTGTTTC TGACTTCATC AAGGCCTTTA TTAAATTCCA CGGTCATGTG 
TACCTGAGTA AGAGCTTGGA AAAGTTGAGC CCTCTTCGAG AGAAATTGGA AGAACAGTTT 
AAGAGGCTGC TATTCCAAAA AGCTTTCAAC TCTCAGCAGT TAGTTCATGT CACTGTCATT 
AACCTGTTTC AACTTCATCA CCTTCGTGAC TTTAGCAATG AAACCGAGCA GCACACTTAT 
AGCCAAGATG AGCAGCTATG TTGGACACAG TTGCTGGCCC TCTTTATGTC TTTTCTTGGC 
ATCCTGTGCA AGTGTCCTCT ACAGAATGAG TCTCAGGAGG AGTCCTACAA TGCCTATCCT 
CTTCCAGCAG TCAAGGTCTC CATGGACTGG CTAAGACTCA GACCCAGGGT CTTTCAGGAG 
GCAGTGGTGG ATGAAAGACA GTACATTTGG CCCTGGTTGA TTTCTCTTCT GAATAGTTTC 
CATCCCCATG AAGAGGACCT CTCAAGTATT AGTGCGACAC CACTTCCAGA GGAGTTTGAA 
TTACAAGGAT TTTTGGCATT GAGACCTTCT TTCAGGAACT TGGATTTTTC CAAAGGTCAC 
CAGGGTATTA CAGGGGACAA AGAAGGCCAG CAACGACGAA TACGACAGCA ACGCTTGATC 
TCTATAGGCA AATGGATTGC TGATAATCAG CCAAGGCTGA TTCAGTGTGA AAATGAGGTA 
GGGAAATTGT TGTTTATCAC AGAAATCCCA GAATTAATAC TGGAAGACCC CAGTGAAGCC 
AAAGAGAACC TCATTCTGCA AGAAACATCT GTGATAGAGT CGCTGGCTGC AGATGGGAGC 
CCAGGGCTAA AATCAGTGCT ATCTACAAGC CGAAATTTAA GCAACAACTG TGACACAGGA 
GAGAAGCCAG TGGTTACCTT CAAAGAAAAC ATTAAGACAC GAGAAGTGAA CAGAGACCAA 
GGAAGAAGTT TTCCTCCCAA AGAGGTAAAA TCCCAGACAG AACTAAGAAA GACTCCAGTG 
TCTGAAGCCA GAAAAACACC TGTAACTCAA ACCCCAACTC AAGCAAGTAA CTCCCAGTTC 
ATCCCCATTC ATCACCCTGG AGCCTTCCCT CCTCTTCCCA GCAGGCCAGG GTTTCCGCCC 
CCAACATATG TTATCCCCCC GCCTGTGGCA TTTTCTATGG GCTCAGGTTA CACCTTCCCA 
GCTGGTGTTT CTGTCCCAGG AACCTTTCTT CAGCCTACAG CTCACTCTCC AGCAGGAAAC 
CAGGTGCAAG CTGGGAAACA GTCCCACATT CCTTACAGCC AGCAACGGCC CTCTGGACCA 
GGGCCAATGA ACCAGGGACC TCAACAATCA CAGCCACCTT CCCAGCAACC CCTTACATCT 
TTACCAGCTC AGCCAACAGC ACAGTCTACA AGCCAGCTGC AGGTTCAAGC TCTAACTCAG 
CAACAACAAT CCCCTACAAA AGCTGTGCCG GCTTTGGGGA AAAGCCCGCC TCACCACTCT 
GGATTCCAGC AGTATCAACA GGCAGATGCC TCCAAACAGC TGTGGAATCC CCCTCAGGTT 
CAAGGCCCAT TAGGGAAAAT TATGCCTGTG AAACAGCCCT ACTACCTTCA GACCCAAGAC 
CCCATAAAAC TGTTTGAGCC GTCATTGCAA CCTCCTGTAA TGCAGCAGCA GCCTCTAGAA 
AAAAAAATGA AGCCTTTTCC CATGGAGCCA TATAACCATA ATCCCTCAGA AGTCAAGGTC 
CCAGAATTCT ACTGGGATTC TTCCTACAGC ATGGCTGATA ACAGATCTGT AATGGCACAG 
CAAGCAAACA TAGACCGCAG GGGCAAACGG TCACCAGGAG TCTTCCGTCC AGAGCAGGAT 
CCTGTACCCA GAATGCCGTT TGAGGACCCC AAGAGCTCCC CTCTGCTTCC TCCGGACCTG 
TTAAAGAGTC TGGCTGCCTT GGAGGAAGAG GAAGAGCTGA TTTTTTCTAA CCCTCCTGAT 
CTTTACCCGG CTCTGCTGGG GCCTCTCGCC TCTCTTCCTG GACGAAGCCT TTTTAAATCC 
TTATTGGAGA AGCCCTCAGA GCTCATGTCA CATTCATCCT CTTTCCTGTC CCTCACCGGA 
TTCTCTCTCA ATCAGGAAAG ATACCCAAAT AATAGTATGT TCAATGAGGT ATATGGGAAA 
AACCTGACAT CCAGCTCCAA AGCAGAACTC AGTCCCTCAA TGGCCCCCCA GGAAACATCT 
CTGTATTCCC TTTTTGAAGG GACTCCGTGG TCTCCATCAC TTCCTGCCAG TTCAGATCAT 
TCAACACCAG CCAGCCAGTC TCCTCATTCC TCTAACCCAA GCAGCCTACC CAGCTCTCCT 
CCAACACACA ACCATAATTC TGTTCCATTC TCCAATTTTG GACCCATTGG GACTCCAGAT 
AACAGGGATA GAAGGACTGC AGATCGGTGG AAAACTGATA AGCCAGCCAT GGGTGGGTTT 
GGCATTGATT ATCTCTCAGC AACGTCATCC TCTGAGAGCA GTTGGCATCA GGCCAGCACT 
CCGAGTGGCA CCTGGACAGG CCATGGCCCT TCCATGGAGG ATTCCTCTGC TGTCCTCATG 
GAAAGCCTAA AGTCTATCTG GTCCAGTTCC ATGATGCATC CTGGACCTTC TGCTCTGGAG 
CAGCTGTTAA TGCAGCAGAA GCAGAAACAG CAACGGGGAC AAGGCACCAT GAACCCTCCA 
CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001362514.1
CDS	123..3548
Translation	MSLQSAQYLRQAEVLKADMTDSKLGPAEVWTSRQALQDLYQKMLVTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRANPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQTHTSAIVKPQSSSCSYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNLQKALSKALESRDEVKTKWGVSDFIKAFIKFHGHVYLSKSLEKLSPLREKLEEQFKRLLFQKAFNSQQLVHVTVINLFQLHHLRDFSNETEQHTYSQDEQLCWTQLLALFMSFLGILCKCPLQNESQEESYNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPHEEDLSSISATPLPEEFELQGFLALRPSFRNLDFSKGHQGITGDKEGQQRRIRQQRLISIGKWIADNQPRLIQCENEVGKLLFITEIPELILEDPSEAKENLILQETSVIESLAADGSPGLKSVLSTSRNLSNNCDTGEKPVVTFKENIKTREVNRDQGRSFPPKEVKSQTELRKTPVSEARKTPVTQTPTQASNSQFIPIHHPGAFPPLPSRPGFPPPTYVIPPPVAFSMGSGYTFPAGVSVPGTFLQPTAHSPAGNQVQAGKQSHIPYSQQRPSGPGPMNQGPQQSQPPSQQPLTSLPAQPTAQSTSQLQVQALTQQQQSPTKAVPALGKSPPHHSGFQQYQQADASKQLWNPPQVQGPLGKIMPVKQPYYLQTQDPIKLFEPSLQPPVMQQQPLEKKMKPFPMEPYNHNPSEVKVPEFYWDSSYSMADNRSVMAQQANIDRRGKRSPGVFRPEQDPVPRMPFEDPKSSPLLPPDLLKSLAALEEEEELIFSNPPDLYPALLGPLASLPGRSLFKSLLEKPSELMSHSSSFLSLTGFSLNQERYPNNSMFNEVYGKNLTSSSKAELSPSMAPQETSLYSLFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSVPFSNFGPIGTPDNRDRRTADRWKTDKPAMGGFGIDYLSATSSSESSWHQASTPSGTWTGHGPSMEDSSAVLMESLKSIWSSSMMHPGPSALEQLLMQQKQKQQRGQGTMNPPH

Target ORF information:

RefSeq Version	NM_001375585.1
Organism	Homo sapiens(human)
Definition	Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001375585.1

ORF Insert Sequence:

ATGAGCCTGC AGAGCGCGCA GTACCTCCGG CAGGCAGAAG TCCTGAAGGC TGACATGACA 
GATTCTAAGC TGGGTCCAGC TGAAGTCTGG ACATCCAGGC AGGCTCTGCA GGACCTGTAC 
CAGAAAATGC TAGTTACCGA TTTGGAATAC GCTTTAGACA AGAAAGTAGA ACAGGATCTC 
TGGAATCACG CCTTTAAGAA TCAGATCACA ACACTGCAAG GCCAGGCAAA GAATCGAGCA 
AATCCGAATC GGAGTGAAGT TCAGGCAAAC CTTTCTCTGT TCCTAGAGGC AGCTAGTGGC 
TTCTATACTC AGTTATTACA AGAACTGTGT ACAGTATTTA ATGTAGATTT ACCATGCCGT 
GTGAAGTCTT CCCAATTGGG AATTATCAGC AATAAACAGA CGCATACCAG CGCCATAGTG 
AAGCCACAGT CTAGCTCCTG TTCCTATATC TGCCAGCACT GCCTCGTCCA CCTTGGAGAC 
ATTGCTCGAT ACAGAAACCA GACCAGCCAG GCAGAGTCCT ACTATAGGCA TGCAGCTCAG 
CTTGTCCCCT CCAATGGTCA GCCTTATAAT CAGTTGGCTA TCTTAGCTTC TTCCAAAGGA 
GACCATCTGA CCACAATTTT CTACTACTGC AGAAGCATTG CTGTGAAGTT CCCTTTCCCA 
GCTGCCTCCA CTAATCTGCA AAAAGCACTT TCTAAAGCAC TGGAAAGCCG AGATGAGGTG 
AAAACCAAGT GGGGTGTTTC TGACTTCATC AAGGCCTTTA TTAAATTCCA CGGTCATGTG 
TACCTGAGTA AGAGCTTGGA AAAGTTGAGC CCTCTTCGAG AGAAATTGGA AGAACAGTTT 
AAGAGGCTGC TATTCCAAAA AGCTTTCAAC TCTCAGCAGT TAGTTCATGT CACTGTCATT 
AACCTGTTTC AACTTCATCA CCTTCGTGAC TTTAGCAATG AAACCGAGCA GCACACTTAT 
AGCCAAGATG AGCAGCTATG TTGGACACAG TTGCTGGCCC TCTTTATGTC TTTTCTTGGC 
ATCCTGTGCA AGTGTCCTCT ACAGAATGAG TCTCAGGAGG AGTCCTACAA TGCCTATCCT 
CTTCCAGCAG TCAAGGTCTC CATGGACTGG CTAAGACTCA GACCCAGGGT CTTTCAGGAG 
GCAGTGGTGG ATGAAAGACA GTACATTTGG CCCTGGTTGA TTTCTCTTCT GAATAGTTTC 
CATCCCCATG AAGAGGACCT CTCAAGTATT AGTGCGACAC CACTTCCAGA GGAGTTTGAA 
TTACAAGGAT TTTTGGCATT GAGACCTTCT TTCAGGAACT TGGATTTTTC CAAAGGTCAC 
CAGGGTATTA CAGGGGACAA AGAAGGCCAG CAACGACGAA TACGACAGCA ACGCTTGATC 
TCTATAGGCA AATGGATTGC TGATAATCAG CCAAGGCTGA TTCAGTGTGA AAATGAGGTA 
GGGAAATTGT TGTTTATCAC AGAAATCCCA GAATTAATAC TGGAAGACCC CAGTGAAGCC 
AAAGAGAACC TCATTCTGCA AGAAACATCT GTGATAGAGT CGCTGGCTGC AGATGGGAGC 
CCAGGGCTAA AATCAGTGCT ATCTACAAGC CGAAATTTAA GCAACAACTG TGACACAGGA 
GAGAAGCCAG TGGTTACCTT CAAAGAAAAC ATTAAGACAC GAGAAGTGAA CAGAGACCAA 
GGAAGAAGTT TTCCTCCCAA AGAGGTAAAA TCCCAGACAG AACTAAGAAA GACTCCAGTG 
TCTGAAGCCA GAAAAACACC TGTAACTCAA ACCCCAACTC AAGCAAGTAA CTCCCAGTTC 
ATCCCCATTC ATCACCCTGG AGCCTTCCCT CCTCTTCCCA GCAGGCCAGG GTTTCCGCCC 
CCAACATATG TTATCCCCCC GCCTGTGGCA TTTTCTATGG GCTCAGGTTA CACCTTCCCA 
GCTGGTGTTT CTGTCCCAGG AACCTTTCTT CAGCCTACAG CTCACTCTCC AGCAGGAAAC 
CAGGTGCAAG CTGGGAAACA GTCCCACATT CCTTACAGCC AGCAACGGCC CTCTGGACCA 
GGGCCAATGA ACCAGGGACC TCAACAATCA CAGCCACCTT CCCAGCAACC CCTTACATCT 
TTACCAGCTC AGCCAACAGC ACAGTCTACA AGCCAGCTGC AGGTTCAAGC TCTAACTCAG 
CAACAACAAT CCCCTACAAA AGCTGTGCCG GCTTTGGGGA AAAGCCCGCC TCACCACTCT 
GGATTCCAGC AGTATCAACA GGCAGATGCC TCCAAACAGC TGTGGAATCC CCCTCAGGTT 
CAAGGCCCAT TAGGGAAAAT TATGCCTGTG AAACAGCCCT ACTACCTTCA GACCCAAGAC 
CCCATAAAAC TGTTTGAGCC GTCATTGCAA CCTCCTGTAA TGCAGCAGCA GCCTCTAGAA 
AAAAAAATGA AGCCTTTTCC CATGGAGCCA TATAACCATA ATCCCTCAGA AGTCAAGGTC 
CCAGAATTCT ACTGGGATTC TTCCTACAGC ATGGCTGATA ACAGATCTGT AATGGCACAG 
CAAGCAAACA TAGACCGCAG GGGCAAACGG TCACCAGGAG TCTTCCGTCC AGAGCAGGAT 
CCTGTACCCA GAATGCCGTT TGAGGACCCC AAGAGCTCCC CTCTGCTTCC TCCGGACCTG 
TTAAAGAGTC TGGCTGCCTT GGAGGAAGAG GAAGAGCTGA TTTTTTCTAA CCCTCCTGAT 
CTTTACCCGG CTCTGCTGGG GCCTCTCGCC TCTCTTCCTG GACGAAGCCT TTTTAAATCC 
TTATTGGAGA AGCCCTCAGA GCTCATGTCA CATTCATCCT CTTTCCTGTC CCTCACCGGA 
TTCTCTCTCA ATCAGGAAAG ATACCCAAAT AATAGTATGT TCAATGAGGT ATATGGGAAA 
AACCTGACAT CCAGCTCCAA AGCAGAACTC AGTCCCTCAA TGGCCCCCCA GGAAACATCT 
CTGTATTCCC TTTTTGAAGG GACTCCGTGG TCTCCATCAC TTCCTGCCAG TTCAGATCAT 
TCAACACCAG CCAGCCAGTC TCCTCATTCC TCTAACCCAA GCAGCCTACC CAGCTCTCCT 
CCAACACACA ACCATAATTC TGTTCCATTC TCCAATTTTG GACCCATTGG GACTCCAGAT 
AACAGGGATA GAAGGACTGC AGATCGGTGG AAAACTGATA AGCCAGCCAT GGGTGGGTTT 
GGCATTGATT ATCTCTCAGC AACGTCATCC TCTGAGAGCA GTTGGCATCA GGCCAGCACT 
CCGAGTGGCA CCTGGACAGG CCATGGCCCT TCCATGGAGG ATTCCTCTGC TGTCCTCATG 
GAAAGCCTAA AGTCTATCTG GTCCAGTTCC ATGATGCATC CTGGACCTTC TGCTCTGGAG 
CAGCTGTTAA TGCAGCAGAA GCAGAAACAG CAACGGGGAC AAGGCACCAT GAACCCTCCA 
CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG7 ( XM_017002968.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016858457.1 Homo sapiens SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X15, mRNA.

Price & Availability↑

CloneID	OHu90882
Clone ID Related Accession (Same CDS sequence)	XM_017002968.1 , XM_017002968.2
Accession Version	XM_017002968.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3150bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	protein SMG7 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTAGTTA CCGATTTGGA ATACGCTTTA GACAAGAAAG TAGAACAGGA TCTCTGGAAT 
CACGCCTTTA AGAATCAGAT CACAACACTG CAAGGCCAGG CAAAGAATCG AGCAAATCCG 
AATCGGAGTG AAGTTCAGGC AAACCTTTCT CTGTTCCTAG AGGCAGCTAG TGGCTTCTAT 
ACTCAGTTAT TACAAGAACT GTGTACAGTA TTTAATGTAG ATTTACCATG CCGTGTGAAG 
TCTTCCCAAT TGGGAATTAT CAGCAATAAA CAGACGCATA CCAGCGCCAT AGTGAAGCCA 
CAGTCTAGCT CCTGTTCCTA TATCTGCCAG CACTGCCTCG TCCACCTTGG AGACATTGCT 
CGATACAGAA ACCAGACCAG CCAGGCAGAG TCCTACTATA GGCATGCAGC TCAGCTTGTC 
CCCTCCAATG GTCAGCCTTA TAATCAGTTG GCTATCTTAG CTTCTTCCAA AGGAGACCAT 
CTGACCACAA TTTTCTACTA CTGCAGAAGC ATTGCTGTGA AGTTCCCTTT CCCAGCTGCC 
TCCACTAATC TGCAAAAAGC ACTTTCTAAA GCACTGGAAA GCCGAGATGA GGTGAAAACC 
AAGTGGGGTG TTTCTGACTT CATCAAGGCC TTTATTAAAT TCCACGGTCA TGTGTACCTG 
AGTAAGAGCT TGGAAAAGTT GAGCCCTCTT CGAGAGAAAT TGGAAGAACA GTTTAAGAGG 
CTGCTATTCC AAAAAGCTTT CAACTCTCAG CAGTTAGTTC ATGTCACTGT CATTAACCTG 
TTTCAACTTC ATCACCTTCG TGACTTTAGC AATGAAACCG AGCAGCACAC TTATAGCCAA 
GATGAGCAGC TATGTTGGAC ACAGTTGCTG GCCCTCTTTA TGTCTTTTCT TGGCATCCTG 
TGCAAGTGTC CTCTACAGAA TGAGTCTCAG GAGGAGTCCT ACAATGCCTA TCCTCTTCCA 
GCAGTCAAGG TCTCCATGGA CTGGCTAAGA CTCAGACCCA GGGTCTTTCA GGAGGCAGTG 
GTGGATGAAA GACAGTACAT TTGGCCCTGG TTGATTTCTC TTCTGAATAG TTTCCATCCC 
CATGAAGAGG ACCTCTCAAG TATTAGTGCG ACACCACTTC CAGAGGAGTT TGAATTACAA 
GGATTTTTGG CATTGAGACC TTCTTTCAGG AACTTGGATT TTTCCAAAGG TCACCAGGGT 
ATTACAGGGG ACAAAGAAGG CCAGCAACGA CGAATACGAC AGCAACGCTT GATCTCTATA 
GGCAAATGGA TTGCTGATAA TCAGCCAAGG CTGATTCAGT GTGAAAATGA GGTAGGGAAA 
TTGTTGTTTA TCACAGAAAT CCCAGAATTA ATACTGGAAG ACCCCAGTGA AGCCAAAGAG 
AACCTCATTC TGCAAGAAAC ATCTGTGATA GAGTCGCTGG CTGCAGATGG GAGCCCAGGG 
CTAAAATCAG TGCTATCTAC AAGCCGAAAT TTAAGCAACA ACTGTGACAC AGGAGAGAAG 
CCAGTGGTTA CCTTCAAAGA AAACATTAAG ACACGAGAAG TGAACAGAGA CCAAGGAAGA 
AGTTTTCCTC CCAAAGAGGT AAAATCCCAG ACAGAACTAA GAAAGACTCC AGTGTCTGAA 
GCCAGAAAAA CACCTGTAAC TCAAACCCCA ACTCAAGCAA GTAACTCCCA GTTCATCCCC 
ATTCATCACC CTGGAGCCTT CCCTCCTCTT CCCAGCAGGC CAGGGTTTCC GCCCCCAACA 
TATGTTATCC CCCCGCCTGT GGCATTTTCT ATGGGCTCAG GTTACACCTT CCCAGCTGGT 
GTTTCTGTCC CAGGAACCTT TCTTCAGCCT ACAGCTCACT CTCCAGCAGG AAACCAGGTG 
CAAGCTGGGA AACAGTCCCA CATTCCTTAC AGCCAGCAAC GGCCCTCTGG ACCAGGGCCA 
ATGAACCAGG GACCTCAACA ATCACAGCCA CCTTCCCAGC AACCCCTTAC ATCTTTACCA 
GCTCAGCCAA CAGCACAGTC TACAAGCCAG CTGCAGGTTC AAGCTCTAAC TCAGCAACAA 
CAATCCCCTA CAAAAGCTGT GCCGGCTTTG GGGAAAAGCC CGCCTCACCA CTCTGGATTC 
CAGCAGTATC AACAGGCAGA TGCCTCCAAA CAGCTGTGGA ATCCCCCTCA GGTTCAAGGC 
CCATTAGGGA AAATTATGCC TGTGAAACAG CCCTACTACC TTCAGACCCA AGACCCCATA 
AAACTGTTTG AGCCGTCATT GCAACCTCCT GTAATGCAGC AGCAGCCTCT AGAAAAAAAA 
ATGAAGCCTT TTCCCATGGA GCCATATAAC CATAATCCCT CAGAAGTCAA GGTCCCAGAA 
TTCTACTGGG ATTCTTCCTA CAGCATGGCT GATAACAGAT CTGTAATGGC ACAGCAAGCA 
AACATAGACC GCAGGGGCAA ACGGTCACCA GGAGTCTTCC GTCCAGAGCA GGATCCTGTA 
CCCAGAATGC CGTTTGAGAA ATCCTTATTG GAGAAGCCCT CAGAGCTCAT GTCACATTCA 
TCCTCTTTCC TGTCCCTCAC CGGATTCTCT CTCAATCAGG AAAGATACCC AAATAATAGT 
ATGTTCAATG AGGTATATGG GAAAAACCTG ACATCCAGCT CCAAAGCAGA ACTCAGTCCC 
TCAATGGCCC CCCAGGAAAC ATCTCTGTAT TCCCTTTTTG AAGGGACTCC GTGGTCTCCA 
TCACTTCCTG CCAGTTCAGA TCATTCAACA CCAGCCAGCC AGTCTCCTCA TTCCTCTAAC 
CCAAGCAGCC TACCCAGCTC TCCTCCAACA CACAACCATA ATTCTGTTCC ATTCTCCAAT 
TTTGGACCCA TTGGGACTCC AGATAACAGG GATAGAAGGA CTGCAGATCG GTGGAAAACT 
GATAAGCCAG CCATGGGTGG GTTTGGCATT GATTATCTCT CAGCAACGTC ATCCTCTGAG 
AGCAGTTGGC ATCAGGCCAG CACTCCGAGT GGCACCTGGA CAGGCCATGG CCCTTCCATG 
GAGGATTCCT CTGCTGTCCT CATGGAAAGC CTAAAGTCTA TCTGGTCCAG TTCCATGATG 
CATCCTGGAC CTTCTGCTCT GGAGCAGCTG TTAATGCAGC AGAAGCAGAA ACAGCAACGG 
GGACAAGGCA CCATGAACCC TCCACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016858457.1
CDS	220..3369
Translation	MLVTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRANPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQTHTSAIVKPQSSSCSYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNLQKALSKALESRDEVKTKWGVSDFIKAFIKFHGHVYLSKSLEKLSPLREKLEEQFKRLLFQKAFNSQQLVHVTVINLFQLHHLRDFSNETEQHTYSQDEQLCWTQLLALFMSFLGILCKCPLQNESQEESYNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPHEEDLSSISATPLPEEFELQGFLALRPSFRNLDFSKGHQGITGDKEGQQRRIRQQRLISIGKWIADNQPRLIQCENEVGKLLFITEIPELILEDPSEAKENLILQETSVIESLAADGSPGLKSVLSTSRNLSNNCDTGEKPVVTFKENIKTREVNRDQGRSFPPKEVKSQTELRKTPVSEARKTPVTQTPTQASNSQFIPIHHPGAFPPLPSRPGFPPPTYVIPPPVAFSMGSGYTFPAGVSVPGTFLQPTAHSPAGNQVQAGKQSHIPYSQQRPSGPGPMNQGPQQSQPPSQQPLTSLPAQPTAQSTSQLQVQALTQQQQSPTKAVPALGKSPPHHSGFQQYQQADASKQLWNPPQVQGPLGKIMPVKQPYYLQTQDPIKLFEPSLQPPVMQQQPLEKKMKPFPMEPYNHNPSEVKVPEFYWDSSYSMADNRSVMAQQANIDRRGKRSPGVFRPEQDPVPRMPFEKSLLEKPSELMSHSSSFLSLTGFSLNQERYPNNSMFNEVYGKNLTSSSKAELSPSMAPQETSLYSLFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSVPFSNFGPIGTPDNRDRRTADRWKTDKPAMGGFGIDYLSATSSSESSWHQASTPSGTWTGHGPSMEDSSAVLMESLKSIWSSSMMHPGPSALEQLLMQQKQKQQRGQGTMNPPH

Target ORF information:

RefSeq Version	XM_017002968.1
Organism	Homo sapiens(human)
Definition	Homo sapiens SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017002968.1

ORF Insert Sequence:

ATGCTAGTTA CCGATTTGGA ATACGCTTTA GACAAGAAAG TAGAACAGGA TCTCTGGAAT 
CACGCCTTTA AGAATCAGAT CACAACACTG CAAGGCCAGG CAAAGAATCG AGCAAATCCG 
AATCGGAGTG AAGTTCAGGC AAACCTTTCT CTGTTCCTAG AGGCAGCTAG TGGCTTCTAT 
ACTCAGTTAT TACAAGAACT GTGTACAGTA TTTAATGTAG ATTTACCATG CCGTGTGAAG 
TCTTCCCAAT TGGGAATTAT CAGCAATAAA CAGACGCATA CCAGCGCCAT AGTGAAGCCA 
CAGTCTAGCT CCTGTTCCTA TATCTGCCAG CACTGCCTCG TCCACCTTGG AGACATTGCT 
CGATACAGAA ACCAGACCAG CCAGGCAGAG TCCTACTATA GGCATGCAGC TCAGCTTGTC 
CCCTCCAATG GTCAGCCTTA TAATCAGTTG GCTATCTTAG CTTCTTCCAA AGGAGACCAT 
CTGACCACAA TTTTCTACTA CTGCAGAAGC ATTGCTGTGA AGTTCCCTTT CCCAGCTGCC 
TCCACTAATC TGCAAAAAGC ACTTTCTAAA GCACTGGAAA GCCGAGATGA GGTGAAAACC 
AAGTGGGGTG TTTCTGACTT CATCAAGGCC TTTATTAAAT TCCACGGTCA TGTGTACCTG 
AGTAAGAGCT TGGAAAAGTT GAGCCCTCTT CGAGAGAAAT TGGAAGAACA GTTTAAGAGG 
CTGCTATTCC AAAAAGCTTT CAACTCTCAG CAGTTAGTTC ATGTCACTGT CATTAACCTG 
TTTCAACTTC ATCACCTTCG TGACTTTAGC AATGAAACCG AGCAGCACAC TTATAGCCAA 
GATGAGCAGC TATGTTGGAC ACAGTTGCTG GCCCTCTTTA TGTCTTTTCT TGGCATCCTG 
TGCAAGTGTC CTCTACAGAA TGAGTCTCAG GAGGAGTCCT ACAATGCCTA TCCTCTTCCA 
GCAGTCAAGG TCTCCATGGA CTGGCTAAGA CTCAGACCCA GGGTCTTTCA GGAGGCAGTG 
GTGGATGAAA GACAGTACAT TTGGCCCTGG TTGATTTCTC TTCTGAATAG TTTCCATCCC 
CATGAAGAGG ACCTCTCAAG TATTAGTGCG ACACCACTTC CAGAGGAGTT TGAATTACAA 
GGATTTTTGG CATTGAGACC TTCTTTCAGG AACTTGGATT TTTCCAAAGG TCACCAGGGT 
ATTACAGGGG ACAAAGAAGG CCAGCAACGA CGAATACGAC AGCAACGCTT GATCTCTATA 
GGCAAATGGA TTGCTGATAA TCAGCCAAGG CTGATTCAGT GTGAAAATGA GGTAGGGAAA 
TTGTTGTTTA TCACAGAAAT CCCAGAATTA ATACTGGAAG ACCCCAGTGA AGCCAAAGAG 
AACCTCATTC TGCAAGAAAC ATCTGTGATA GAGTCGCTGG CTGCAGATGG GAGCCCAGGG 
CTAAAATCAG TGCTATCTAC AAGCCGAAAT TTAAGCAACA ACTGTGACAC AGGAGAGAAG 
CCAGTGGTTA CCTTCAAAGA AAACATTAAG ACACGAGAAG TGAACAGAGA CCAAGGAAGA 
AGTTTTCCTC CCAAAGAGGT AAAATCCCAG ACAGAACTAA GAAAGACTCC AGTGTCTGAA 
GCCAGAAAAA CACCTGTAAC TCAAACCCCA ACTCAAGCAA GTAACTCCCA GTTCATCCCC 
ATTCATCACC CTGGAGCCTT CCCTCCTCTT CCCAGCAGGC CAGGGTTTCC GCCCCCAACA 
TATGTTATCC CCCCGCCTGT GGCATTTTCT ATGGGCTCAG GTTACACCTT CCCAGCTGGT 
GTTTCTGTCC CAGGAACCTT TCTTCAGCCT ACAGCTCACT CTCCAGCAGG AAACCAGGTG 
CAAGCTGGGA AACAGTCCCA CATTCCTTAC AGCCAGCAAC GGCCCTCTGG ACCAGGGCCA 
ATGAACCAGG GACCTCAACA ATCACAGCCA CCTTCCCAGC AACCCCTTAC ATCTTTACCA 
GCTCAGCCAA CAGCACAGTC TACAAGCCAG CTGCAGGTTC AAGCTCTAAC TCAGCAACAA 
CAATCCCCTA CAAAAGCTGT GCCGGCTTTG GGGAAAAGCC CGCCTCACCA CTCTGGATTC 
CAGCAGTATC AACAGGCAGA TGCCTCCAAA CAGCTGTGGA ATCCCCCTCA GGTTCAAGGC 
CCATTAGGGA AAATTATGCC TGTGAAACAG CCCTACTACC TTCAGACCCA AGACCCCATA 
AAACTGTTTG AGCCGTCATT GCAACCTCCT GTAATGCAGC AGCAGCCTCT AGAAAAAAAA 
ATGAAGCCTT TTCCCATGGA GCCATATAAC CATAATCCCT CAGAAGTCAA GGTCCCAGAA 
TTCTACTGGG ATTCTTCCTA CAGCATGGCT GATAACAGAT CTGTAATGGC ACAGCAAGCA 
AACATAGACC GCAGGGGCAA ACGGTCACCA GGAGTCTTCC GTCCAGAGCA GGATCCTGTA 
CCCAGAATGC CGTTTGAGAA ATCCTTATTG GAGAAGCCCT CAGAGCTCAT GTCACATTCA 
TCCTCTTTCC TGTCCCTCAC CGGATTCTCT CTCAATCAGG AAAGATACCC AAATAATAGT 
ATGTTCAATG AGGTATATGG GAAAAACCTG ACATCCAGCT CCAAAGCAGA ACTCAGTCCC 
TCAATGGCCC CCCAGGAAAC ATCTCTGTAT TCCCTTTTTG AAGGGACTCC GTGGTCTCCA 
TCACTTCCTG CCAGTTCAGA TCATTCAACA CCAGCCAGCC AGTCTCCTCA TTCCTCTAAC 
CCAAGCAGCC TACCCAGCTC TCCTCCAACA CACAACCATA ATTCTGTTCC ATTCTCCAAT 
TTTGGACCCA TTGGGACTCC AGATAACAGG GATAGAAGGA CTGCAGATCG GTGGAAAACT 
GATAAGCCAG CCATGGGTGG GTTTGGCATT GATTATCTCT CAGCAACGTC ATCCTCTGAG 
AGCAGTTGGC ATCAGGCCAG CACTCCGAGT GGCACCTGGA CAGGCCATGG CCCTTCCATG 
GAGGATTCCT CTGCTGTCCT CATGGAAAGC CTAAAGTCTA TCTGGTCCAG TTCCATGATG 
CATCCTGGAC CTTCTGCTCT GGAGCAGCTG TTAATGCAGC AGAAGCAGAA ACAGCAACGG 
GGACAAGGCA CCATGAACCC TCCACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG7 ( XM_017002968.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016858457.1 Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X11, mRNA.

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CloneID	OHu90882
Clone ID Related Accession (Same CDS sequence)	XM_017002968.1 , XM_017002968.2
Accession Version	XM_017002968.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3150bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein SMG7 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017002968.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTAGTTA CCGATTTGGA ATACGCTTTA GACAAGAAAG TAGAACAGGA TCTCTGGAAT 
CACGCCTTTA AGAATCAGAT CACAACACTG CAAGGCCAGG CAAAGAATCG AGCAAATCCG 
AATCGGAGTG AAGTTCAGGC AAACCTTTCT CTGTTCCTAG AGGCAGCTAG TGGCTTCTAT 
ACTCAGTTAT TACAAGAACT GTGTACAGTA TTTAATGTAG ATTTACCATG CCGTGTGAAG 
TCTTCCCAAT TGGGAATTAT CAGCAATAAA CAGACGCATA CCAGCGCCAT AGTGAAGCCA 
CAGTCTAGCT CCTGTTCCTA TATCTGCCAG CACTGCCTCG TCCACCTTGG AGACATTGCT 
CGATACAGAA ACCAGACCAG CCAGGCAGAG TCCTACTATA GGCATGCAGC TCAGCTTGTC 
CCCTCCAATG GTCAGCCTTA TAATCAGTTG GCTATCTTAG CTTCTTCCAA AGGAGACCAT 
CTGACCACAA TTTTCTACTA CTGCAGAAGC ATTGCTGTGA AGTTCCCTTT CCCAGCTGCC 
TCCACTAATC TGCAAAAAGC ACTTTCTAAA GCACTGGAAA GCCGAGATGA GGTGAAAACC 
AAGTGGGGTG TTTCTGACTT CATCAAGGCC TTTATTAAAT TCCACGGTCA TGTGTACCTG 
AGTAAGAGCT TGGAAAAGTT GAGCCCTCTT CGAGAGAAAT TGGAAGAACA GTTTAAGAGG 
CTGCTATTCC AAAAAGCTTT CAACTCTCAG CAGTTAGTTC ATGTCACTGT CATTAACCTG 
TTTCAACTTC ATCACCTTCG TGACTTTAGC AATGAAACCG AGCAGCACAC TTATAGCCAA 
GATGAGCAGC TATGTTGGAC ACAGTTGCTG GCCCTCTTTA TGTCTTTTCT TGGCATCCTG 
TGCAAGTGTC CTCTACAGAA TGAGTCTCAG GAGGAGTCCT ACAATGCCTA TCCTCTTCCA 
GCAGTCAAGG TCTCCATGGA CTGGCTAAGA CTCAGACCCA GGGTCTTTCA GGAGGCAGTG 
GTGGATGAAA GACAGTACAT TTGGCCCTGG TTGATTTCTC TTCTGAATAG TTTCCATCCC 
CATGAAGAGG ACCTCTCAAG TATTAGTGCG ACACCACTTC CAGAGGAGTT TGAATTACAA 
GGATTTTTGG CATTGAGACC TTCTTTCAGG AACTTGGATT TTTCCAAAGG TCACCAGGGT 
ATTACAGGGG ACAAAGAAGG CCAGCAACGA CGAATACGAC AGCAACGCTT GATCTCTATA 
GGCAAATGGA TTGCTGATAA TCAGCCAAGG CTGATTCAGT GTGAAAATGA GGTAGGGAAA 
TTGTTGTTTA TCACAGAAAT CCCAGAATTA ATACTGGAAG ACCCCAGTGA AGCCAAAGAG 
AACCTCATTC TGCAAGAAAC ATCTGTGATA GAGTCGCTGG CTGCAGATGG GAGCCCAGGG 
CTAAAATCAG TGCTATCTAC AAGCCGAAAT TTAAGCAACA ACTGTGACAC AGGAGAGAAG 
CCAGTGGTTA CCTTCAAAGA AAACATTAAG ACACGAGAAG TGAACAGAGA CCAAGGAAGA 
AGTTTTCCTC CCAAAGAGGT AAAATCCCAG ACAGAACTAA GAAAGACTCC AGTGTCTGAA 
GCCAGAAAAA CACCTGTAAC TCAAACCCCA ACTCAAGCAA GTAACTCCCA GTTCATCCCC 
ATTCATCACC CTGGAGCCTT CCCTCCTCTT CCCAGCAGGC CAGGGTTTCC GCCCCCAACA 
TATGTTATCC CCCCGCCTGT GGCATTTTCT ATGGGCTCAG GTTACACCTT CCCAGCTGGT 
GTTTCTGTCC CAGGAACCTT TCTTCAGCCT ACAGCTCACT CTCCAGCAGG AAACCAGGTG 
CAAGCTGGGA AACAGTCCCA CATTCCTTAC AGCCAGCAAC GGCCCTCTGG ACCAGGGCCA 
ATGAACCAGG GACCTCAACA ATCACAGCCA CCTTCCCAGC AACCCCTTAC ATCTTTACCA 
GCTCAGCCAA CAGCACAGTC TACAAGCCAG CTGCAGGTTC AAGCTCTAAC TCAGCAACAA 
CAATCCCCTA CAAAAGCTGT GCCGGCTTTG GGGAAAAGCC CGCCTCACCA CTCTGGATTC 
CAGCAGTATC AACAGGCAGA TGCCTCCAAA CAGCTGTGGA ATCCCCCTCA GGTTCAAGGC 
CCATTAGGGA AAATTATGCC TGTGAAACAG CCCTACTACC TTCAGACCCA AGACCCCATA 
AAACTGTTTG AGCCGTCATT GCAACCTCCT GTAATGCAGC AGCAGCCTCT AGAAAAAAAA 
ATGAAGCCTT TTCCCATGGA GCCATATAAC CATAATCCCT CAGAAGTCAA GGTCCCAGAA 
TTCTACTGGG ATTCTTCCTA CAGCATGGCT GATAACAGAT CTGTAATGGC ACAGCAAGCA 
AACATAGACC GCAGGGGCAA ACGGTCACCA GGAGTCTTCC GTCCAGAGCA GGATCCTGTA 
CCCAGAATGC CGTTTGAGAA ATCCTTATTG GAGAAGCCCT CAGAGCTCAT GTCACATTCA 
TCCTCTTTCC TGTCCCTCAC CGGATTCTCT CTCAATCAGG AAAGATACCC AAATAATAGT 
ATGTTCAATG AGGTATATGG GAAAAACCTG ACATCCAGCT CCAAAGCAGA ACTCAGTCCC 
TCAATGGCCC CCCAGGAAAC ATCTCTGTAT TCCCTTTTTG AAGGGACTCC GTGGTCTCCA 
TCACTTCCTG CCAGTTCAGA TCATTCAACA CCAGCCAGCC AGTCTCCTCA TTCCTCTAAC 
CCAAGCAGCC TACCCAGCTC TCCTCCAACA CACAACCATA ATTCTGTTCC ATTCTCCAAT 
TTTGGACCCA TTGGGACTCC AGATAACAGG GATAGAAGGA CTGCAGATCG GTGGAAAACT 
GATAAGCCAG CCATGGGTGG GTTTGGCATT GATTATCTCT CAGCAACGTC ATCCTCTGAG 
AGCAGTTGGC ATCAGGCCAG CACTCCGAGT GGCACCTGGA CAGGCCATGG CCCTTCCATG 
GAGGATTCCT CTGCTGTCCT CATGGAAAGC CTAAAGTCTA TCTGGTCCAG TTCCATGATG 
CATCCTGGAC CTTCTGCTCT GGAGCAGCTG TTAATGCAGC AGAAGCAGAA ACAGCAACGG 
GGACAAGGCA CCATGAACCC TCCACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016858457.1
CDS	214..3363
Translation	MLVTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRANPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQTHTSAIVKPQSSSCSYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNLQKALSKALESRDEVKTKWGVSDFIKAFIKFHGHVYLSKSLEKLSPLREKLEEQFKRLLFQKAFNSQQLVHVTVINLFQLHHLRDFSNETEQHTYSQDEQLCWTQLLALFMSFLGILCKCPLQNESQEESYNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPHEEDLSSISATPLPEEFELQGFLALRPSFRNLDFSKGHQGITGDKEGQQRRIRQQRLISIGKWIADNQPRLIQCENEVGKLLFITEIPELILEDPSEAKENLILQETSVIESLAADGSPGLKSVLSTSRNLSNNCDTGEKPVVTFKENIKTREVNRDQGRSFPPKEVKSQTELRKTPVSEARKTPVTQTPTQASNSQFIPIHHPGAFPPLPSRPGFPPPTYVIPPPVAFSMGSGYTFPAGVSVPGTFLQPTAHSPAGNQVQAGKQSHIPYSQQRPSGPGPMNQGPQQSQPPSQQPLTSLPAQPTAQSTSQLQVQALTQQQQSPTKAVPALGKSPPHHSGFQQYQQADASKQLWNPPQVQGPLGKIMPVKQPYYLQTQDPIKLFEPSLQPPVMQQQPLEKKMKPFPMEPYNHNPSEVKVPEFYWDSSYSMADNRSVMAQQANIDRRGKRSPGVFRPEQDPVPRMPFEKSLLEKPSELMSHSSSFLSLTGFSLNQERYPNNSMFNEVYGKNLTSSSKAELSPSMAPQETSLYSLFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSVPFSNFGPIGTPDNRDRRTADRWKTDKPAMGGFGIDYLSATSSSESSWHQASTPSGTWTGHGPSMEDSSAVLMESLKSIWSSSMMHPGPSALEQLLMQQKQKQQRGQGTMNPPH

Target ORF information:

RefSeq Version	XM_017002968.2
Organism	Homo sapiens(human)
Definition	Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017002968.2

ORF Insert Sequence:

ATGCTAGTTA CCGATTTGGA ATACGCTTTA GACAAGAAAG TAGAACAGGA TCTCTGGAAT 
CACGCCTTTA AGAATCAGAT CACAACACTG CAAGGCCAGG CAAAGAATCG AGCAAATCCG 
AATCGGAGTG AAGTTCAGGC AAACCTTTCT CTGTTCCTAG AGGCAGCTAG TGGCTTCTAT 
ACTCAGTTAT TACAAGAACT GTGTACAGTA TTTAATGTAG ATTTACCATG CCGTGTGAAG 
TCTTCCCAAT TGGGAATTAT CAGCAATAAA CAGACGCATA CCAGCGCCAT AGTGAAGCCA 
CAGTCTAGCT CCTGTTCCTA TATCTGCCAG CACTGCCTCG TCCACCTTGG AGACATTGCT 
CGATACAGAA ACCAGACCAG CCAGGCAGAG TCCTACTATA GGCATGCAGC TCAGCTTGTC 
CCCTCCAATG GTCAGCCTTA TAATCAGTTG GCTATCTTAG CTTCTTCCAA AGGAGACCAT 
CTGACCACAA TTTTCTACTA CTGCAGAAGC ATTGCTGTGA AGTTCCCTTT CCCAGCTGCC 
TCCACTAATC TGCAAAAAGC ACTTTCTAAA GCACTGGAAA GCCGAGATGA GGTGAAAACC 
AAGTGGGGTG TTTCTGACTT CATCAAGGCC TTTATTAAAT TCCACGGTCA TGTGTACCTG 
AGTAAGAGCT TGGAAAAGTT GAGCCCTCTT CGAGAGAAAT TGGAAGAACA GTTTAAGAGG 
CTGCTATTCC AAAAAGCTTT CAACTCTCAG CAGTTAGTTC ATGTCACTGT CATTAACCTG 
TTTCAACTTC ATCACCTTCG TGACTTTAGC AATGAAACCG AGCAGCACAC TTATAGCCAA 
GATGAGCAGC TATGTTGGAC ACAGTTGCTG GCCCTCTTTA TGTCTTTTCT TGGCATCCTG 
TGCAAGTGTC CTCTACAGAA TGAGTCTCAG GAGGAGTCCT ACAATGCCTA TCCTCTTCCA 
GCAGTCAAGG TCTCCATGGA CTGGCTAAGA CTCAGACCCA GGGTCTTTCA GGAGGCAGTG 
GTGGATGAAA GACAGTACAT TTGGCCCTGG TTGATTTCTC TTCTGAATAG TTTCCATCCC 
CATGAAGAGG ACCTCTCAAG TATTAGTGCG ACACCACTTC CAGAGGAGTT TGAATTACAA 
GGATTTTTGG CATTGAGACC TTCTTTCAGG AACTTGGATT TTTCCAAAGG TCACCAGGGT 
ATTACAGGGG ACAAAGAAGG CCAGCAACGA CGAATACGAC AGCAACGCTT GATCTCTATA 
GGCAAATGGA TTGCTGATAA TCAGCCAAGG CTGATTCAGT GTGAAAATGA GGTAGGGAAA 
TTGTTGTTTA TCACAGAAAT CCCAGAATTA ATACTGGAAG ACCCCAGTGA AGCCAAAGAG 
AACCTCATTC TGCAAGAAAC ATCTGTGATA GAGTCGCTGG CTGCAGATGG GAGCCCAGGG 
CTAAAATCAG TGCTATCTAC AAGCCGAAAT TTAAGCAACA ACTGTGACAC AGGAGAGAAG 
CCAGTGGTTA CCTTCAAAGA AAACATTAAG ACACGAGAAG TGAACAGAGA CCAAGGAAGA 
AGTTTTCCTC CCAAAGAGGT AAAATCCCAG ACAGAACTAA GAAAGACTCC AGTGTCTGAA 
GCCAGAAAAA CACCTGTAAC TCAAACCCCA ACTCAAGCAA GTAACTCCCA GTTCATCCCC 
ATTCATCACC CTGGAGCCTT CCCTCCTCTT CCCAGCAGGC CAGGGTTTCC GCCCCCAACA 
TATGTTATCC CCCCGCCTGT GGCATTTTCT ATGGGCTCAG GTTACACCTT CCCAGCTGGT 
GTTTCTGTCC CAGGAACCTT TCTTCAGCCT ACAGCTCACT CTCCAGCAGG AAACCAGGTG 
CAAGCTGGGA AACAGTCCCA CATTCCTTAC AGCCAGCAAC GGCCCTCTGG ACCAGGGCCA 
ATGAACCAGG GACCTCAACA ATCACAGCCA CCTTCCCAGC AACCCCTTAC ATCTTTACCA 
GCTCAGCCAA CAGCACAGTC TACAAGCCAG CTGCAGGTTC AAGCTCTAAC TCAGCAACAA 
CAATCCCCTA CAAAAGCTGT GCCGGCTTTG GGGAAAAGCC CGCCTCACCA CTCTGGATTC 
CAGCAGTATC AACAGGCAGA TGCCTCCAAA CAGCTGTGGA ATCCCCCTCA GGTTCAAGGC 
CCATTAGGGA AAATTATGCC TGTGAAACAG CCCTACTACC TTCAGACCCA AGACCCCATA 
AAACTGTTTG AGCCGTCATT GCAACCTCCT GTAATGCAGC AGCAGCCTCT AGAAAAAAAA 
ATGAAGCCTT TTCCCATGGA GCCATATAAC CATAATCCCT CAGAAGTCAA GGTCCCAGAA 
TTCTACTGGG ATTCTTCCTA CAGCATGGCT GATAACAGAT CTGTAATGGC ACAGCAAGCA 
AACATAGACC GCAGGGGCAA ACGGTCACCA GGAGTCTTCC GTCCAGAGCA GGATCCTGTA 
CCCAGAATGC CGTTTGAGAA ATCCTTATTG GAGAAGCCCT CAGAGCTCAT GTCACATTCA 
TCCTCTTTCC TGTCCCTCAC CGGATTCTCT CTCAATCAGG AAAGATACCC AAATAATAGT 
ATGTTCAATG AGGTATATGG GAAAAACCTG ACATCCAGCT CCAAAGCAGA ACTCAGTCCC 
TCAATGGCCC CCCAGGAAAC ATCTCTGTAT TCCCTTTTTG AAGGGACTCC GTGGTCTCCA 
TCACTTCCTG CCAGTTCAGA TCATTCAACA CCAGCCAGCC AGTCTCCTCA TTCCTCTAAC 
CCAAGCAGCC TACCCAGCTC TCCTCCAACA CACAACCATA ATTCTGTTCC ATTCTCCAAT 
TTTGGACCCA TTGGGACTCC AGATAACAGG GATAGAAGGA CTGCAGATCG GTGGAAAACT 
GATAAGCCAG CCATGGGTGG GTTTGGCATT GATTATCTCT CAGCAACGTC ATCCTCTGAG 
AGCAGTTGGC ATCAGGCCAG CACTCCGAGT GGCACCTGGA CAGGCCATGG CCCTTCCATG 
GAGGATTCCT CTGCTGTCCT CATGGAAAGC CTAAAGTCTA TCTGGTCCAG TTCCATGATG 
CATCCTGGAC CTTCTGCTCT GGAGCAGCTG TTAATGCAGC AGAAGCAGAA ACAGCAACGG 
GGACAAGGCA CCATGAACCC TCCACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG7 ( NM_201568.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_963862.1 Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu02898
Clone ID Related Accession (Same CDS sequence)	NM_201568.3 , NM_201568.2
Accession Version	NM_201568.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3276bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-28
Organism	Homo sapiens(human)
Product	protein SMG7 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB223038.1, AB085674.1, DC380923.1, D87437.2 and CN479993.1. On Jun 2, 2019 this sequence version replaced NM_201568.2. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7346977.2287091.1, SRR7346977.2824553.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCCTGC AGAGCGCGCA GTACCTCCGG CAGGCAGAAG TCCTGAAGGC TGACATGACA 
GATTCTAAGC TGGGTCCAGC TGAAGTCTGG ACATCCAGGC AGGCTCTGCA GGACCTGTAC 
CAGAAAATGC TAGTTACCGA TTTGGAATAC GCTTTAGACA AGAAAGTAGA ACAGGATCTC 
TGGAATCACG CCTTTAAGAA TCAGATCACA ACACTGCAAG GCCAGGCAAA GAATCGAGCA 
AATCCGAATC GGAGTGAAGT TCAGGCAAAC CTTTCTCTGT TCCTAGAGGC AGCTAGTGGC 
TTCTATACTC AGTTATTACA AGAACTGTGT ACAGTATTTA ATGTAGATTT ACCATGCCGT 
GTGAAGTCTT CCCAATTGGG AATTATCAGC AATAAACAGA CGCATACCAG CGCCATAGTG 
AAGCCACAGT CTAGCTCCTG TTCCTATATC TGCCAGCACT GCCTCGTCCA CCTTGGAGAC 
ATTGCTCGAT ACAGAAACCA GACCAGCCAG GCAGAGTCCT ACTATAGGCA TGCAGCTCAG 
CTTGTCCCCT CCAATGGTCA GCCTTATAAT CAGTTGGCTA TCTTAGCTTC TTCCAAAGGA 
GACCATCTGA CCACAATTTT CTACTACTGC AGAAGCATTG CTGTGAAGTT CCCTTTCCCA 
GCTGCCTCCA CTAATCTGCA AAAAGCACTT TCTAAAGCAC TGGAAAGCCG AGATGAGGTG 
AAAACCAAGT GGGGTGTTTC TGACTTCATC AAGGCCTTTA TTAAATTCCA CGGTCATGTG 
TACCTGAGTA AGAGCTTGGA AAAGTTGAGC CCTCTTCGAG AGAAATTGGA AGAACAGTTT 
AAGAGGCTGC TATTCCAAAA AGCTTTCAAC TCTCAGCAGT TAGTTCATGT CACTGTCATT 
AACCTGTTTC AACTTCATCA CCTTCGTGAC TTTAGCAATG AAACCGAGCA GCACACTTAT 
AGCCAAGATG AGCAGCTATG TTGGACACAG TTGCTGGCCC TCTTTATGTC TTTTCTTGGC 
ATCCTGTGCA AGTGTCCTCT ACAGAATGAG TCTCAGGAGG AGTCCTACAA TGCCTATCCT 
CTTCCAGCAG TCAAGGTCTC CATGGACTGG CTAAGACTCA GACCCAGGGT CTTTCAGGAG 
GCAGTGGTGG ATGAAAGACA GTACATTTGG CCCTGGTTGA TTTCTCTTCT GAATAGTTTC 
CATCCCCATG AAGAGGACCT CTCAAGTATT AGTGCGACAC CACTTCCAGA GGAGTTTGAA 
TTACAAGGAT TTTTGGCATT GAGACCTTCT TTCAGGAACT TGGATTTTTC CAAAGGTCAC 
CAGGGTATTA CAGGGGACAA AGAAGGCCAG CAACGACGAA TACGACAGCA ACGCTTGATC 
TCTATAGGCA AATGGATTGC TGATAATCAG CCAAGGCTGA TTCAGTGTGA AAATGAGGTA 
GGGAAATTGT TGTTTATCAC AGAAATCCCA GAATTAATAC TGGAAGACCC CAGTGAAGCC 
AAAGAGAACC TCATTCTGCA AGAAACATCT GTGATAGAGT CGCTGGCTGC AGATGGGAGC 
CCAGGGCTAA AATCAGTGCT ATCTACAAGC CGAAATTTAA GCAACAACTG TGACACAGGA 
GAGAAGCCAG TGGTTACCTT CAAAGAAAAC ATTAAGACAC GAGAAGTGAA CAGAGACCAA 
GGAAGAAGTT TTCCTCCCAA AGAGGTAAAA TCCCAGACAG AACTAAGAAA GACTCCAGTG 
TCTGAAGCCA GAAAAACACC TGTAACTCAA ACCCCAACTC AAGCAAGTAA CTCCCAGTTC 
ATCCCCATTC ATCACCCTGG AGCCTTCCCT CCTCTTCCCA GCAGGCCAGG GTTTCCGCCC 
CCAACATATG TTATCCCCCC GCCTGTGGCA TTTTCTATGG GCTCAGGTTA CACCTTCCCA 
GCTGGTGTTT CTGTCCCAGG AACCTTTCTT CAGCCTACAG CTCACTCTCC AGCAGGAAAC 
CAGGTGCAAG CTGGGAAACA GTCCCACATT CCTTACAGCC AGCAACGGCC CTCTGGACCA 
GGGCCAATGA ACCAGGGACC TCAACAATCA CAGCCACCTT CCCAGCAACC CCTTACATCT 
TTACCAGCTC AGCCAACAGC ACAGTCTACA AGCCAGCTGC AGGTTCAAGC TCTAACTCAG 
CAACAACAAT CCCCTACAAA AGCTGTGCCG GCTTTGGGGA AAAGCCCGCC TCACCACTCT 
GGATTCCAGC AGTATCAACA GGCAGATGCC TCCAAACAGC TGTGGAATCC CCCTCAGGTT 
CAAGGCCCAT TAGGGAAAAT TATGCCTGTG AAACAGCCCT ACTACCTTCA GACCCAAGAC 
CCCATAAAAC TGTTTGAGCC GTCATTGCAA CCTCCTGTAA TGCAGCAGCA GCCTCTAGAA 
AAAAAAATGA AGCCTTTTCC CATGGAGCCA TATAACCATA ATCCCTCAGA AGTCAAGGTC 
CCAGAATTCT ACTGGGATTC TTCCTACAGC ATGGCTGATA ACAGATCTGT AATGGCACAG 
CAAGCAAACA TAGACCGCAG GGGCAAACGG TCACCAGGAG TCTTCCGTCC AGAGCAGGAT 
CCTGTACCCA GAATGCCGTT TGAGAAATCC TTATTGGAGA AGCCCTCAGA GCTCATGTCA 
CATTCATCCT CTTTCCTGTC CCTCACCGGA TTCTCTCTCA ATCAGGAAAG ATACCCAAAT 
AATAGTATGT TCAATGAGGT ATATGGGAAA AACCTGACAT CCAGCTCCAA AGCAGAACTC 
AGTCCCTCAA TGGCCCCCCA GGAAACATCT CTGTATTCCC TTTTTGAAGG GACTCCGTGG 
TCTCCATCAC TTCCTGCCAG TTCAGATCAT TCAACACCAG CCAGCCAGTC TCCTCATTCC 
TCTAACCCAA GCAGCCTACC CAGCTCTCCT CCAACACACA ACCATAATTC TGTTCCATTC 
TCCAATTTTG GACCCATTGG GACTCCAGAT AACAGGGATA GAAGGACTGC AGATCGGTGG 
AAAACTGATA AGCCAGCCAT GGGTGGGTTT GGCATTGATT ATCTCTCAGC AACGTCATCC 
TCTGAGAGCA GTTGGCATCA GGCCAGCACT CCGAGTGGCA CCTGGACAGG CCATGGCCCT 
TCCATGGAGG ATTCCTCTGC TGTCCTCATG GAAAGCCTAA AGTCTATCTG GTCCAGTTCC 
ATGATGCATC CTGGACCTTC TGCTCTGGAG CAGCTGTTAA TGCAGCAGAA GCAGAAACAG 
CAACGGGGAC AAGGCACCAT GAACCCTCCA CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_963862.1
CDS	123..3398
Translation	MSLQSAQYLRQAEVLKADMTDSKLGPAEVWTSRQALQDLYQKMLVTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRANPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQTHTSAIVKPQSSSCSYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNLQKALSKALESRDEVKTKWGVSDFIKAFIKFHGHVYLSKSLEKLSPLREKLEEQFKRLLFQKAFNSQQLVHVTVINLFQLHHLRDFSNETEQHTYSQDEQLCWTQLLALFMSFLGILCKCPLQNESQEESYNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPHEEDLSSISATPLPEEFELQGFLALRPSFRNLDFSKGHQGITGDKEGQQRRIRQQRLISIGKWIADNQPRLIQCENEVGKLLFITEIPELILEDPSEAKENLILQETSVIESLAADGSPGLKSVLSTSRNLSNNCDTGEKPVVTFKENIKTREVNRDQGRSFPPKEVKSQTELRKTPVSEARKTPVTQTPTQASNSQFIPIHHPGAFPPLPSRPGFPPPTYVIPPPVAFSMGSGYTFPAGVSVPGTFLQPTAHSPAGNQVQAGKQSHIPYSQQRPSGPGPMNQGPQQSQPPSQQPLTSLPAQPTAQSTSQLQVQALTQQQQSPTKAVPALGKSPPHHSGFQQYQQADASKQLWNPPQVQGPLGKIMPVKQPYYLQTQDPIKLFEPSLQPPVMQQQPLEKKMKPFPMEPYNHNPSEVKVPEFYWDSSYSMADNRSVMAQQANIDRRGKRSPGVFRPEQDPVPRMPFEKSLLEKPSELMSHSSSFLSLTGFSLNQERYPNNSMFNEVYGKNLTSSSKAELSPSMAPQETSLYSLFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSVPFSNFGPIGTPDNRDRRTADRWKTDKPAMGGFGIDYLSATSSSESSWHQASTPSGTWTGHGPSMEDSSAVLMESLKSIWSSSMMHPGPSALEQLLMQQKQKQQRGQGTMNPPH

Target ORF information:

RefSeq Version	NM_201568.3
Organism	Homo sapiens(human)
Definition	Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_201568.3

ORF Insert Sequence:

ATGAGCCTGC AGAGCGCGCA GTACCTCCGG CAGGCAGAAG TCCTGAAGGC TGACATGACA 
GATTCTAAGC TGGGTCCAGC TGAAGTCTGG ACATCCAGGC AGGCTCTGCA GGACCTGTAC 
CAGAAAATGC TAGTTACCGA TTTGGAATAC GCTTTAGACA AGAAAGTAGA ACAGGATCTC 
TGGAATCACG CCTTTAAGAA TCAGATCACA ACACTGCAAG GCCAGGCAAA GAATCGAGCA 
AATCCGAATC GGAGTGAAGT TCAGGCAAAC CTTTCTCTGT TCCTAGAGGC AGCTAGTGGC 
TTCTATACTC AGTTATTACA AGAACTGTGT ACAGTATTTA ATGTAGATTT ACCATGCCGT 
GTGAAGTCTT CCCAATTGGG AATTATCAGC AATAAACAGA CGCATACCAG CGCCATAGTG 
AAGCCACAGT CTAGCTCCTG TTCCTATATC TGCCAGCACT GCCTCGTCCA CCTTGGAGAC 
ATTGCTCGAT ACAGAAACCA GACCAGCCAG GCAGAGTCCT ACTATAGGCA TGCAGCTCAG 
CTTGTCCCCT CCAATGGTCA GCCTTATAAT CAGTTGGCTA TCTTAGCTTC TTCCAAAGGA 
GACCATCTGA CCACAATTTT CTACTACTGC AGAAGCATTG CTGTGAAGTT CCCTTTCCCA 
GCTGCCTCCA CTAATCTGCA AAAAGCACTT TCTAAAGCAC TGGAAAGCCG AGATGAGGTG 
AAAACCAAGT GGGGTGTTTC TGACTTCATC AAGGCCTTTA TTAAATTCCA CGGTCATGTG 
TACCTGAGTA AGAGCTTGGA AAAGTTGAGC CCTCTTCGAG AGAAATTGGA AGAACAGTTT 
AAGAGGCTGC TATTCCAAAA AGCTTTCAAC TCTCAGCAGT TAGTTCATGT CACTGTCATT 
AACCTGTTTC AACTTCATCA CCTTCGTGAC TTTAGCAATG AAACCGAGCA GCACACTTAT 
AGCCAAGATG AGCAGCTATG TTGGACACAG TTGCTGGCCC TCTTTATGTC TTTTCTTGGC 
ATCCTGTGCA AGTGTCCTCT ACAGAATGAG TCTCAGGAGG AGTCCTACAA TGCCTATCCT 
CTTCCAGCAG TCAAGGTCTC CATGGACTGG CTAAGACTCA GACCCAGGGT CTTTCAGGAG 
GCAGTGGTGG ATGAAAGACA GTACATTTGG CCCTGGTTGA TTTCTCTTCT GAATAGTTTC 
CATCCCCATG AAGAGGACCT CTCAAGTATT AGTGCGACAC CACTTCCAGA GGAGTTTGAA 
TTACAAGGAT TTTTGGCATT GAGACCTTCT TTCAGGAACT TGGATTTTTC CAAAGGTCAC 
CAGGGTATTA CAGGGGACAA AGAAGGCCAG CAACGACGAA TACGACAGCA ACGCTTGATC 
TCTATAGGCA AATGGATTGC TGATAATCAG CCAAGGCTGA TTCAGTGTGA AAATGAGGTA 
GGGAAATTGT TGTTTATCAC AGAAATCCCA GAATTAATAC TGGAAGACCC CAGTGAAGCC 
AAAGAGAACC TCATTCTGCA AGAAACATCT GTGATAGAGT CGCTGGCTGC AGATGGGAGC 
CCAGGGCTAA AATCAGTGCT ATCTACAAGC CGAAATTTAA GCAACAACTG TGACACAGGA 
GAGAAGCCAG TGGTTACCTT CAAAGAAAAC ATTAAGACAC GAGAAGTGAA CAGAGACCAA 
GGAAGAAGTT TTCCTCCCAA AGAGGTAAAA TCCCAGACAG AACTAAGAAA GACTCCAGTG 
TCTGAAGCCA GAAAAACACC TGTAACTCAA ACCCCAACTC AAGCAAGTAA CTCCCAGTTC 
ATCCCCATTC ATCACCCTGG AGCCTTCCCT CCTCTTCCCA GCAGGCCAGG GTTTCCGCCC 
CCAACATATG TTATCCCCCC GCCTGTGGCA TTTTCTATGG GCTCAGGTTA CACCTTCCCA 
GCTGGTGTTT CTGTCCCAGG AACCTTTCTT CAGCCTACAG CTCACTCTCC AGCAGGAAAC 
CAGGTGCAAG CTGGGAAACA GTCCCACATT CCTTACAGCC AGCAACGGCC CTCTGGACCA 
GGGCCAATGA ACCAGGGACC TCAACAATCA CAGCCACCTT CCCAGCAACC CCTTACATCT 
TTACCAGCTC AGCCAACAGC ACAGTCTACA AGCCAGCTGC AGGTTCAAGC TCTAACTCAG 
CAACAACAAT CCCCTACAAA AGCTGTGCCG GCTTTGGGGA AAAGCCCGCC TCACCACTCT 
GGATTCCAGC AGTATCAACA GGCAGATGCC TCCAAACAGC TGTGGAATCC CCCTCAGGTT 
CAAGGCCCAT TAGGGAAAAT TATGCCTGTG AAACAGCCCT ACTACCTTCA GACCCAAGAC 
CCCATAAAAC TGTTTGAGCC GTCATTGCAA CCTCCTGTAA TGCAGCAGCA GCCTCTAGAA 
AAAAAAATGA AGCCTTTTCC CATGGAGCCA TATAACCATA ATCCCTCAGA AGTCAAGGTC 
CCAGAATTCT ACTGGGATTC TTCCTACAGC ATGGCTGATA ACAGATCTGT AATGGCACAG 
CAAGCAAACA TAGACCGCAG GGGCAAACGG TCACCAGGAG TCTTCCGTCC AGAGCAGGAT 
CCTGTACCCA GAATGCCGTT TGAGAAATCC TTATTGGAGA AGCCCTCAGA GCTCATGTCA 
CATTCATCCT CTTTCCTGTC CCTCACCGGA TTCTCTCTCA ATCAGGAAAG ATACCCAAAT 
AATAGTATGT TCAATGAGGT ATATGGGAAA AACCTGACAT CCAGCTCCAA AGCAGAACTC 
AGTCCCTCAA TGGCCCCCCA GGAAACATCT CTGTATTCCC TTTTTGAAGG GACTCCGTGG 
TCTCCATCAC TTCCTGCCAG TTCAGATCAT TCAACACCAG CCAGCCAGTC TCCTCATTCC 
TCTAACCCAA GCAGCCTACC CAGCTCTCCT CCAACACACA ACCATAATTC TGTTCCATTC 
TCCAATTTTG GACCCATTGG GACTCCAGAT AACAGGGATA GAAGGACTGC AGATCGGTGG 
AAAACTGATA AGCCAGCCAT GGGTGGGTTT GGCATTGATT ATCTCTCAGC AACGTCATCC 
TCTGAGAGCA GTTGGCATCA GGCCAGCACT CCGAGTGGCA CCTGGACAGG CCATGGCCCT 
TCCATGGAGG ATTCCTCTGC TGTCCTCATG GAAAGCCTAA AGTCTATCTG GTCCAGTTCC 
ATGATGCATC CTGGACCTTC TGCTCTGGAG CAGCTGTTAA TGCAGCAGAA GCAGAAACAG 
CAACGGGGAC AAGGCACCAT GAACCCTCCA CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG7 ( NM_201568.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_963862.1 Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu02898
Clone ID Related Accession (Same CDS sequence)	NM_201568.3 , NM_201568.2
Accession Version	NM_201568.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3276bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-27
Organism	Homo sapiens(human)
Product	protein SMG7 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB223038.1, AB085674.1, DC380923.1, D87437.2 and CN479993.1. On Mar 23, 2010 this sequence version replaced NM_201568.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D87437.2, SRR1803614.66164.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCCTGC AGAGCGCGCA GTACCTCCGG CAGGCAGAAG TCCTGAAGGC TGACATGACA 
GATTCTAAGC TGGGTCCAGC TGAAGTCTGG ACATCCAGGC AGGCTCTGCA GGACCTGTAC 
CAGAAAATGC TAGTTACCGA TTTGGAATAC GCTTTAGACA AGAAAGTAGA ACAGGATCTC 
TGGAATCACG CCTTTAAGAA TCAGATCACA ACACTGCAAG GCCAGGCAAA GAATCGAGCA 
AATCCGAATC GGAGTGAAGT TCAGGCAAAC CTTTCTCTGT TCCTAGAGGC AGCTAGTGGC 
TTCTATACTC AGTTATTACA AGAACTGTGT ACAGTATTTA ATGTAGATTT ACCATGCCGT 
GTGAAGTCTT CCCAATTGGG AATTATCAGC AATAAACAGA CGCATACCAG CGCCATAGTG 
AAGCCACAGT CTAGCTCCTG TTCCTATATC TGCCAGCACT GCCTCGTCCA CCTTGGAGAC 
ATTGCTCGAT ACAGAAACCA GACCAGCCAG GCAGAGTCCT ACTATAGGCA TGCAGCTCAG 
CTTGTCCCCT CCAATGGTCA GCCTTATAAT CAGTTGGCTA TCTTAGCTTC TTCCAAAGGA 
GACCATCTGA CCACAATTTT CTACTACTGC AGAAGCATTG CTGTGAAGTT CCCTTTCCCA 
GCTGCCTCCA CTAATCTGCA AAAAGCACTT TCTAAAGCAC TGGAAAGCCG AGATGAGGTG 
AAAACCAAGT GGGGTGTTTC TGACTTCATC AAGGCCTTTA TTAAATTCCA CGGTCATGTG 
TACCTGAGTA AGAGCTTGGA AAAGTTGAGC CCTCTTCGAG AGAAATTGGA AGAACAGTTT 
AAGAGGCTGC TATTCCAAAA AGCTTTCAAC TCTCAGCAGT TAGTTCATGT CACTGTCATT 
AACCTGTTTC AACTTCATCA CCTTCGTGAC TTTAGCAATG AAACCGAGCA GCACACTTAT 
AGCCAAGATG AGCAGCTATG TTGGACACAG TTGCTGGCCC TCTTTATGTC TTTTCTTGGC 
ATCCTGTGCA AGTGTCCTCT ACAGAATGAG TCTCAGGAGG AGTCCTACAA TGCCTATCCT 
CTTCCAGCAG TCAAGGTCTC CATGGACTGG CTAAGACTCA GACCCAGGGT CTTTCAGGAG 
GCAGTGGTGG ATGAAAGACA GTACATTTGG CCCTGGTTGA TTTCTCTTCT GAATAGTTTC 
CATCCCCATG AAGAGGACCT CTCAAGTATT AGTGCGACAC CACTTCCAGA GGAGTTTGAA 
TTACAAGGAT TTTTGGCATT GAGACCTTCT TTCAGGAACT TGGATTTTTC CAAAGGTCAC 
CAGGGTATTA CAGGGGACAA AGAAGGCCAG CAACGACGAA TACGACAGCA ACGCTTGATC 
TCTATAGGCA AATGGATTGC TGATAATCAG CCAAGGCTGA TTCAGTGTGA AAATGAGGTA 
GGGAAATTGT TGTTTATCAC AGAAATCCCA GAATTAATAC TGGAAGACCC CAGTGAAGCC 
AAAGAGAACC TCATTCTGCA AGAAACATCT GTGATAGAGT CGCTGGCTGC AGATGGGAGC 
CCAGGGCTAA AATCAGTGCT ATCTACAAGC CGAAATTTAA GCAACAACTG TGACACAGGA 
GAGAAGCCAG TGGTTACCTT CAAAGAAAAC ATTAAGACAC GAGAAGTGAA CAGAGACCAA 
GGAAGAAGTT TTCCTCCCAA AGAGGTAAAA TCCCAGACAG AACTAAGAAA GACTCCAGTG 
TCTGAAGCCA GAAAAACACC TGTAACTCAA ACCCCAACTC AAGCAAGTAA CTCCCAGTTC 
ATCCCCATTC ATCACCCTGG AGCCTTCCCT CCTCTTCCCA GCAGGCCAGG GTTTCCGCCC 
CCAACATATG TTATCCCCCC GCCTGTGGCA TTTTCTATGG GCTCAGGTTA CACCTTCCCA 
GCTGGTGTTT CTGTCCCAGG AACCTTTCTT CAGCCTACAG CTCACTCTCC AGCAGGAAAC 
CAGGTGCAAG CTGGGAAACA GTCCCACATT CCTTACAGCC AGCAACGGCC CTCTGGACCA 
GGGCCAATGA ACCAGGGACC TCAACAATCA CAGCCACCTT CCCAGCAACC CCTTACATCT 
TTACCAGCTC AGCCAACAGC ACAGTCTACA AGCCAGCTGC AGGTTCAAGC TCTAACTCAG 
CAACAACAAT CCCCTACAAA AGCTGTGCCG GCTTTGGGGA AAAGCCCGCC TCACCACTCT 
GGATTCCAGC AGTATCAACA GGCAGATGCC TCCAAACAGC TGTGGAATCC CCCTCAGGTT 
CAAGGCCCAT TAGGGAAAAT TATGCCTGTG AAACAGCCCT ACTACCTTCA GACCCAAGAC 
CCCATAAAAC TGTTTGAGCC GTCATTGCAA CCTCCTGTAA TGCAGCAGCA GCCTCTAGAA 
AAAAAAATGA AGCCTTTTCC CATGGAGCCA TATAACCATA ATCCCTCAGA AGTCAAGGTC 
CCAGAATTCT ACTGGGATTC TTCCTACAGC ATGGCTGATA ACAGATCTGT AATGGCACAG 
CAAGCAAACA TAGACCGCAG GGGCAAACGG TCACCAGGAG TCTTCCGTCC AGAGCAGGAT 
CCTGTACCCA GAATGCCGTT TGAGAAATCC TTATTGGAGA AGCCCTCAGA GCTCATGTCA 
CATTCATCCT CTTTCCTGTC CCTCACCGGA TTCTCTCTCA ATCAGGAAAG ATACCCAAAT 
AATAGTATGT TCAATGAGGT ATATGGGAAA AACCTGACAT CCAGCTCCAA AGCAGAACTC 
AGTCCCTCAA TGGCCCCCCA GGAAACATCT CTGTATTCCC TTTTTGAAGG GACTCCGTGG 
TCTCCATCAC TTCCTGCCAG TTCAGATCAT TCAACACCAG CCAGCCAGTC TCCTCATTCC 
TCTAACCCAA GCAGCCTACC CAGCTCTCCT CCAACACACA ACCATAATTC TGTTCCATTC 
TCCAATTTTG GACCCATTGG GACTCCAGAT AACAGGGATA GAAGGACTGC AGATCGGTGG 
AAAACTGATA AGCCAGCCAT GGGTGGGTTT GGCATTGATT ATCTCTCAGC AACGTCATCC 
TCTGAGAGCA GTTGGCATCA GGCCAGCACT CCGAGTGGCA CCTGGACAGG CCATGGCCCT 
TCCATGGAGG ATTCCTCTGC TGTCCTCATG GAAAGCCTAA AGTCTATCTG GTCCAGTTCC 
ATGATGCATC CTGGACCTTC TGCTCTGGAG CAGCTGTTAA TGCAGCAGAA GCAGAAACAG 
CAACGGGGAC AAGGCACCAT GAACCCTCCA CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_963862.1
CDS	251..3526
Translation	MSLQSAQYLRQAEVLKADMTDSKLGPAEVWTSRQALQDLYQKMLVTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRANPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQTHTSAIVKPQSSSCSYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNLQKALSKALESRDEVKTKWGVSDFIKAFIKFHGHVYLSKSLEKLSPLREKLEEQFKRLLFQKAFNSQQLVHVTVINLFQLHHLRDFSNETEQHTYSQDEQLCWTQLLALFMSFLGILCKCPLQNESQEESYNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPHEEDLSSISATPLPEEFELQGFLALRPSFRNLDFSKGHQGITGDKEGQQRRIRQQRLISIGKWIADNQPRLIQCENEVGKLLFITEIPELILEDPSEAKENLILQETSVIESLAADGSPGLKSVLSTSRNLSNNCDTGEKPVVTFKENIKTREVNRDQGRSFPPKEVKSQTELRKTPVSEARKTPVTQTPTQASNSQFIPIHHPGAFPPLPSRPGFPPPTYVIPPPVAFSMGSGYTFPAGVSVPGTFLQPTAHSPAGNQVQAGKQSHIPYSQQRPSGPGPMNQGPQQSQPPSQQPLTSLPAQPTAQSTSQLQVQALTQQQQSPTKAVPALGKSPPHHSGFQQYQQADASKQLWNPPQVQGPLGKIMPVKQPYYLQTQDPIKLFEPSLQPPVMQQQPLEKKMKPFPMEPYNHNPSEVKVPEFYWDSSYSMADNRSVMAQQANIDRRGKRSPGVFRPEQDPVPRMPFEKSLLEKPSELMSHSSSFLSLTGFSLNQERYPNNSMFNEVYGKNLTSSSKAELSPSMAPQETSLYSLFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSVPFSNFGPIGTPDNRDRRTADRWKTDKPAMGGFGIDYLSATSSSESSWHQASTPSGTWTGHGPSMEDSSAVLMESLKSIWSSSMMHPGPSALEQLLMQQKQKQQRGQGTMNPPH

Target ORF information:

RefSeq Version	NM_201568.2
Organism	Homo sapiens(human)
Definition	Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_201568.2

ORF Insert Sequence:

ATGAGCCTGC AGAGCGCGCA GTACCTCCGG CAGGCAGAAG TCCTGAAGGC TGACATGACA 
GATTCTAAGC TGGGTCCAGC TGAAGTCTGG ACATCCAGGC AGGCTCTGCA GGACCTGTAC 
CAGAAAATGC TAGTTACCGA TTTGGAATAC GCTTTAGACA AGAAAGTAGA ACAGGATCTC 
TGGAATCACG CCTTTAAGAA TCAGATCACA ACACTGCAAG GCCAGGCAAA GAATCGAGCA 
AATCCGAATC GGAGTGAAGT TCAGGCAAAC CTTTCTCTGT TCCTAGAGGC AGCTAGTGGC 
TTCTATACTC AGTTATTACA AGAACTGTGT ACAGTATTTA ATGTAGATTT ACCATGCCGT 
GTGAAGTCTT CCCAATTGGG AATTATCAGC AATAAACAGA CGCATACCAG CGCCATAGTG 
AAGCCACAGT CTAGCTCCTG TTCCTATATC TGCCAGCACT GCCTCGTCCA CCTTGGAGAC 
ATTGCTCGAT ACAGAAACCA GACCAGCCAG GCAGAGTCCT ACTATAGGCA TGCAGCTCAG 
CTTGTCCCCT CCAATGGTCA GCCTTATAAT CAGTTGGCTA TCTTAGCTTC TTCCAAAGGA 
GACCATCTGA CCACAATTTT CTACTACTGC AGAAGCATTG CTGTGAAGTT CCCTTTCCCA 
GCTGCCTCCA CTAATCTGCA AAAAGCACTT TCTAAAGCAC TGGAAAGCCG AGATGAGGTG 
AAAACCAAGT GGGGTGTTTC TGACTTCATC AAGGCCTTTA TTAAATTCCA CGGTCATGTG 
TACCTGAGTA AGAGCTTGGA AAAGTTGAGC CCTCTTCGAG AGAAATTGGA AGAACAGTTT 
AAGAGGCTGC TATTCCAAAA AGCTTTCAAC TCTCAGCAGT TAGTTCATGT CACTGTCATT 
AACCTGTTTC AACTTCATCA CCTTCGTGAC TTTAGCAATG AAACCGAGCA GCACACTTAT 
AGCCAAGATG AGCAGCTATG TTGGACACAG TTGCTGGCCC TCTTTATGTC TTTTCTTGGC 
ATCCTGTGCA AGTGTCCTCT ACAGAATGAG TCTCAGGAGG AGTCCTACAA TGCCTATCCT 
CTTCCAGCAG TCAAGGTCTC CATGGACTGG CTAAGACTCA GACCCAGGGT CTTTCAGGAG 
GCAGTGGTGG ATGAAAGACA GTACATTTGG CCCTGGTTGA TTTCTCTTCT GAATAGTTTC 
CATCCCCATG AAGAGGACCT CTCAAGTATT AGTGCGACAC CACTTCCAGA GGAGTTTGAA 
TTACAAGGAT TTTTGGCATT GAGACCTTCT TTCAGGAACT TGGATTTTTC CAAAGGTCAC 
CAGGGTATTA CAGGGGACAA AGAAGGCCAG CAACGACGAA TACGACAGCA ACGCTTGATC 
TCTATAGGCA AATGGATTGC TGATAATCAG CCAAGGCTGA TTCAGTGTGA AAATGAGGTA 
GGGAAATTGT TGTTTATCAC AGAAATCCCA GAATTAATAC TGGAAGACCC CAGTGAAGCC 
AAAGAGAACC TCATTCTGCA AGAAACATCT GTGATAGAGT CGCTGGCTGC AGATGGGAGC 
CCAGGGCTAA AATCAGTGCT ATCTACAAGC CGAAATTTAA GCAACAACTG TGACACAGGA 
GAGAAGCCAG TGGTTACCTT CAAAGAAAAC ATTAAGACAC GAGAAGTGAA CAGAGACCAA 
GGAAGAAGTT TTCCTCCCAA AGAGGTAAAA TCCCAGACAG AACTAAGAAA GACTCCAGTG 
TCTGAAGCCA GAAAAACACC TGTAACTCAA ACCCCAACTC AAGCAAGTAA CTCCCAGTTC 
ATCCCCATTC ATCACCCTGG AGCCTTCCCT CCTCTTCCCA GCAGGCCAGG GTTTCCGCCC 
CCAACATATG TTATCCCCCC GCCTGTGGCA TTTTCTATGG GCTCAGGTTA CACCTTCCCA 
GCTGGTGTTT CTGTCCCAGG AACCTTTCTT CAGCCTACAG CTCACTCTCC AGCAGGAAAC 
CAGGTGCAAG CTGGGAAACA GTCCCACATT CCTTACAGCC AGCAACGGCC CTCTGGACCA 
GGGCCAATGA ACCAGGGACC TCAACAATCA CAGCCACCTT CCCAGCAACC CCTTACATCT 
TTACCAGCTC AGCCAACAGC ACAGTCTACA AGCCAGCTGC AGGTTCAAGC TCTAACTCAG 
CAACAACAAT CCCCTACAAA AGCTGTGCCG GCTTTGGGGA AAAGCCCGCC TCACCACTCT 
GGATTCCAGC AGTATCAACA GGCAGATGCC TCCAAACAGC TGTGGAATCC CCCTCAGGTT 
CAAGGCCCAT TAGGGAAAAT TATGCCTGTG AAACAGCCCT ACTACCTTCA GACCCAAGAC 
CCCATAAAAC TGTTTGAGCC GTCATTGCAA CCTCCTGTAA TGCAGCAGCA GCCTCTAGAA 
AAAAAAATGA AGCCTTTTCC CATGGAGCCA TATAACCATA ATCCCTCAGA AGTCAAGGTC 
CCAGAATTCT ACTGGGATTC TTCCTACAGC ATGGCTGATA ACAGATCTGT AATGGCACAG 
CAAGCAAACA TAGACCGCAG GGGCAAACGG TCACCAGGAG TCTTCCGTCC AGAGCAGGAT 
CCTGTACCCA GAATGCCGTT TGAGAAATCC TTATTGGAGA AGCCCTCAGA GCTCATGTCA 
CATTCATCCT CTTTCCTGTC CCTCACCGGA TTCTCTCTCA ATCAGGAAAG ATACCCAAAT 
AATAGTATGT TCAATGAGGT ATATGGGAAA AACCTGACAT CCAGCTCCAA AGCAGAACTC 
AGTCCCTCAA TGGCCCCCCA GGAAACATCT CTGTATTCCC TTTTTGAAGG GACTCCGTGG 
TCTCCATCAC TTCCTGCCAG TTCAGATCAT TCAACACCAG CCAGCCAGTC TCCTCATTCC 
TCTAACCCAA GCAGCCTACC CAGCTCTCCT CCAACACACA ACCATAATTC TGTTCCATTC 
TCCAATTTTG GACCCATTGG GACTCCAGAT AACAGGGATA GAAGGACTGC AGATCGGTGG 
AAAACTGATA AGCCAGCCAT GGGTGGGTTT GGCATTGATT ATCTCTCAGC AACGTCATCC 
TCTGAGAGCA GTTGGCATCA GGCCAGCACT CCGAGTGGCA CCTGGACAGG CCATGGCCCT 
TCCATGGAGG ATTCCTCTGC TGTCCTCATG GAAAGCCTAA AGTCTATCTG GTCCAGTTCC 
ATGATGCATC CTGGACCTTC TGCTCTGGAG CAGCTGTTAA TGCAGCAGAA GCAGAAACAG 
CAACGGGGAC AAGGCACCAT GAACCCTCCA CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG7 ( NM_173156.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_775179.1 Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu07205
Clone ID Related Accession (Same CDS sequence)	NM_173156.2 , NM_173156.3
Accession Version	NM_173156.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3414bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	protein SMG7 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB223038.1, AB085674.1, DC380923.1, AY386363.1 and CN479993.1. On Mar 23, 2010 this sequence version replaced NM_173156.1. Transcript Variant: This variant (1) encodes isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.233309.1, AB085674.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCCTGC AGAGCGCGCA GTACCTCCGG CAGGCAGAAG TCCTGAAGGC TGACATGACA 
GATTCTAAGC TGGGTCCAGC TGAAGTCTGG ACATCCAGGC AGGCTCTGCA GGACCTGTAC 
CAGAAAATGC TAGTTACCGA TTTGGAATAC GCTTTAGACA AGAAAGTAGA ACAGGATCTC 
TGGAATCACG CCTTTAAGAA TCAGATCACA ACACTGCAAG GCCAGGCAAA GAATCGAGCA 
AATCCGAATC GGAGTGAAGT TCAGGCAAAC CTTTCTCTGT TCCTAGAGGC AGCTAGTGGC 
TTCTATACTC AGTTATTACA AGAACTGTGT ACAGTATTTA ATGTAGATTT ACCATGCCGT 
GTGAAGTCTT CCCAATTGGG AATTATCAGC AATAAACAGA CGCATACCAG CGCCATAGTG 
AAGCCACAGT CTAGCTCCTG TTCCTATATC TGCCAGCACT GCCTCGTCCA CCTTGGAGAC 
ATTGCTCGAT ACAGAAACCA GACCAGCCAG GCAGAGTCCT ACTATAGGCA TGCAGCTCAG 
CTTGTCCCCT CCAATGGTCA GCCTTATAAT CAGTTGGCTA TCTTAGCTTC TTCCAAAGGA 
GACCATCTGA CCACAATTTT CTACTACTGC AGAAGCATTG CTGTGAAGTT CCCTTTCCCA 
GCTGCCTCCA CTAATCTGCA AAAAGCACTT TCTAAAGCAC TGGAAAGCCG AGATGAGGTG 
AAAACCAAGT GGGGTGTTTC TGACTTCATC AAGGCCTTTA TTAAATTCCA CGGTCATGTG 
TACCTGAGTA AGAGCTTGGA AAAGTTGAGC CCTCTTCGAG AGAAATTGGA AGAACAGTTT 
AAGAGGCTGC TATTCCAAAA AGCTTTCAAC TCTCAGCAGT TAGTTCATGT CACTGTCATT 
AACCTGTTTC AACTTCATCA CCTTCGTGAC TTTAGCAATG AAACCGAGCA GCACACTTAT 
AGCCAAGATG AGCAGCTATG TTGGACACAG TTGCTGGCCC TCTTTATGTC TTTTCTTGGC 
ATCCTGTGCA AGTGTCCTCT ACAGAATGAG TCTCAGGAGG AGTCCTACAA TGCCTATCCT 
CTTCCAGCAG TCAAGGTCTC CATGGACTGG CTAAGACTCA GACCCAGGGT CTTTCAGGAG 
GCAGTGGTGG ATGAAAGACA GTACATTTGG CCCTGGTTGA TTTCTCTTCT GAATAGTTTC 
CATCCCCATG AAGAGGACCT CTCAAGTATT AGTGCGACAC CACTTCCAGA GGAGTTTGAA 
TTACAAGGAT TTTTGGCATT GAGACCTTCT TTCAGGAACT TGGATTTTTC CAAAGGTCAC 
CAGGGTATTA CAGGGGACAA AGAAGGCCAG CAACGACGAA TACGACAGCA ACGCTTGATC 
TCTATAGGCA AATGGATTGC TGATAATCAG CCAAGGCTGA TTCAGTGTGA AAATGAGGTA 
GGGAAATTGT TGTTTATCAC AGAAATCCCA GAATTAATAC TGGAAGACCC CAGTGAAGCC 
AAAGAGAACC TCATTCTGCA AGAAACATCT GTGATAGAGT CGCTGGCTGC AGATGGGAGC 
CCAGGGCTAA AATCAGTGCT ATCTACAAGC CGAAATTTAA GCAACAACTG TGACACAGGA 
GAGAAGCCAG TGGTTACCTT CAAAGAAAAC ATTAAGACAC GAGAAGTGAA CAGAGACCAA 
GGAAGAAGTT TTCCTCCCAA AGAGGTGAGA AGGGACTATA GCAAAGGAAT AACTGTAACT 
AAGAATGATG GAAAGAAGGA CAACAACAAG AGGAAAACTG AAACCAAGAA ATGCACCTTA 
GAAAAGTTAC AGGAAACAGG AAAGCAGAAT GTGGCAGTGC AGGTAAAATC CCAGACAGAA 
CTAAGAAAGA CTCCAGTGTC TGAAGCCAGA AAAACACCTG TAACTCAAAC CCCAACTCAA 
GCAAGTAACT CCCAGTTCAT CCCCATTCAT CACCCTGGAG CCTTCCCTCC TCTTCCCAGC 
AGGCCAGGGT TTCCGCCCCC AACATATGTT ATCCCCCCGC CTGTGGCATT TTCTATGGGC 
TCAGGTTACA CCTTCCCAGC TGGTGTTTCT GTCCCAGGAA CCTTTCTTCA GCCTACAGCT 
CACTCTCCAG CAGGAAACCA GGTGCAAGCT GGGAAACAGT CCCACATTCC TTACAGCCAG 
CAACGGCCCT CTGGACCAGG GCCAATGAAC CAGGGACCTC AACAATCACA GCCACCTTCC 
CAGCAACCCC TTACATCTTT ACCAGCTCAG CCAACAGCAC AGTCTACAAG CCAGCTGCAG 
GTTCAAGCTC TAACTCAGCA ACAACAATCC CCTACAAAAG CTGTGCCGGC TTTGGGGAAA 
AGCCCGCCTC ACCACTCTGG ATTCCAGCAG TATCAACAGG CAGATGCCTC CAAACAGCTG 
TGGAATCCCC CTCAGGTTCA AGGCCCATTA GGGAAAATTA TGCCTGTGAA ACAGCCCTAC 
TACCTTCAGA CCCAAGACCC CATAAAACTG TTTGAGCCGT CATTGCAACC TCCTGTAATG 
CAGCAGCAGC CTCTAGAAAA AAAAATGAAG CCTTTTCCCA TGGAGCCATA TAACCATAAT 
CCCTCAGAAG TCAAGGTCCC AGAATTCTAC TGGGATTCTT CCTACAGCAT GGCTGATAAC 
AGATCTGTAA TGGCACAGCA AGCAAACATA GACCGCAGGG GCAAACGGTC ACCAGGAGTC 
TTCCGTCCAG AGCAGGATCC TGTACCCAGA ATGCCGTTTG AGAAATCCTT ATTGGAGAAG 
CCCTCAGAGC TCATGTCACA TTCATCCTCT TTCCTGTCCC TCACCGGATT CTCTCTCAAT 
CAGGAAAGAT ACCCAAATAA TAGTATGTTC AATGAGGTAT ATGGGAAAAA CCTGACATCC 
AGCTCCAAAG CAGAACTCAG TCCCTCAATG GCCCCCCAGG AAACATCTCT GTATTCCCTT 
TTTGAAGGGA CTCCGTGGTC TCCATCACTT CCTGCCAGTT CAGATCATTC AACACCAGCC 
AGCCAGTCTC CTCATTCCTC TAACCCAAGC AGCCTACCCA GCTCTCCTCC AACACACAAC 
CATAATTCTG TTCCATTCTC CAATTTTGGA CCCATTGGGA CTCCAGATAA CAGGGATAGA 
AGGACTGCAG ATCGGTGGAA AACTGATAAG CCAGCCATGG GTGGGTTTGG CATTGATTAT 
CTCTCAGCAA CGTCATCCTC TGAGAGCAGT TGGCATCAGG CCAGCACTCC GAGTGGCACC 
TGGACAGGCC ATGGCCCTTC CATGGAGGAT TCCTCTGCTG TCCTCATGGA AAGCCTAAAG 
TCTATCTGGT CCAGTTCCAT GATGCATCCT GGACCTTCTG CTCTGGAGCA GCTGTTAATG 
CAGCAGAAGC AGAAACAGCA ACGGGGACAA GGCACCATGA ACCCTCCACA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_775179.1
CDS	251..3664
Translation	MSLQSAQYLRQAEVLKADMTDSKLGPAEVWTSRQALQDLYQKMLVTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRANPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQTHTSAIVKPQSSSCSYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNLQKALSKALESRDEVKTKWGVSDFIKAFIKFHGHVYLSKSLEKLSPLREKLEEQFKRLLFQKAFNSQQLVHVTVINLFQLHHLRDFSNETEQHTYSQDEQLCWTQLLALFMSFLGILCKCPLQNESQEESYNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPHEEDLSSISATPLPEEFELQGFLALRPSFRNLDFSKGHQGITGDKEGQQRRIRQQRLISIGKWIADNQPRLIQCENEVGKLLFITEIPELILEDPSEAKENLILQETSVIESLAADGSPGLKSVLSTSRNLSNNCDTGEKPVVTFKENIKTREVNRDQGRSFPPKEVRRDYSKGITVTKNDGKKDNNKRKTETKKCTLEKLQETGKQNVAVQVKSQTELRKTPVSEARKTPVTQTPTQASNSQFIPIHHPGAFPPLPSRPGFPPPTYVIPPPVAFSMGSGYTFPAGVSVPGTFLQPTAHSPAGNQVQAGKQSHIPYSQQRPSGPGPMNQGPQQSQPPSQQPLTSLPAQPTAQSTSQLQVQALTQQQQSPTKAVPALGKSPPHHSGFQQYQQADASKQLWNPPQVQGPLGKIMPVKQPYYLQTQDPIKLFEPSLQPPVMQQQPLEKKMKPFPMEPYNHNPSEVKVPEFYWDSSYSMADNRSVMAQQANIDRRGKRSPGVFRPEQDPVPRMPFEKSLLEKPSELMSHSSSFLSLTGFSLNQERYPNNSMFNEVYGKNLTSSSKAELSPSMAPQETSLYSLFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSVPFSNFGPIGTPDNRDRRTADRWKTDKPAMGGFGIDYLSATSSSESSWHQASTPSGTWTGHGPSMEDSSAVLMESLKSIWSSSMMHPGPSALEQLLMQQKQKQQRGQGTMNPPH

Target ORF information:

RefSeq Version	NM_173156.2
Organism	Homo sapiens(human)
Definition	Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_173156.2

ORF Insert Sequence:

ATGAGCCTGC AGAGCGCGCA GTACCTCCGG CAGGCAGAAG TCCTGAAGGC TGACATGACA 
GATTCTAAGC TGGGTCCAGC TGAAGTCTGG ACATCCAGGC AGGCTCTGCA GGACCTGTAC 
CAGAAAATGC TAGTTACCGA TTTGGAATAC GCTTTAGACA AGAAAGTAGA ACAGGATCTC 
TGGAATCACG CCTTTAAGAA TCAGATCACA ACACTGCAAG GCCAGGCAAA GAATCGAGCA 
AATCCGAATC GGAGTGAAGT TCAGGCAAAC CTTTCTCTGT TCCTAGAGGC AGCTAGTGGC 
TTCTATACTC AGTTATTACA AGAACTGTGT ACAGTATTTA ATGTAGATTT ACCATGCCGT 
GTGAAGTCTT CCCAATTGGG AATTATCAGC AATAAACAGA CGCATACCAG CGCCATAGTG 
AAGCCACAGT CTAGCTCCTG TTCCTATATC TGCCAGCACT GCCTCGTCCA CCTTGGAGAC 
ATTGCTCGAT ACAGAAACCA GACCAGCCAG GCAGAGTCCT ACTATAGGCA TGCAGCTCAG 
CTTGTCCCCT CCAATGGTCA GCCTTATAAT CAGTTGGCTA TCTTAGCTTC TTCCAAAGGA 
GACCATCTGA CCACAATTTT CTACTACTGC AGAAGCATTG CTGTGAAGTT CCCTTTCCCA 
GCTGCCTCCA CTAATCTGCA AAAAGCACTT TCTAAAGCAC TGGAAAGCCG AGATGAGGTG 
AAAACCAAGT GGGGTGTTTC TGACTTCATC AAGGCCTTTA TTAAATTCCA CGGTCATGTG 
TACCTGAGTA AGAGCTTGGA AAAGTTGAGC CCTCTTCGAG AGAAATTGGA AGAACAGTTT 
AAGAGGCTGC TATTCCAAAA AGCTTTCAAC TCTCAGCAGT TAGTTCATGT CACTGTCATT 
AACCTGTTTC AACTTCATCA CCTTCGTGAC TTTAGCAATG AAACCGAGCA GCACACTTAT 
AGCCAAGATG AGCAGCTATG TTGGACACAG TTGCTGGCCC TCTTTATGTC TTTTCTTGGC 
ATCCTGTGCA AGTGTCCTCT ACAGAATGAG TCTCAGGAGG AGTCCTACAA TGCCTATCCT 
CTTCCAGCAG TCAAGGTCTC CATGGACTGG CTAAGACTCA GACCCAGGGT CTTTCAGGAG 
GCAGTGGTGG ATGAAAGACA GTACATTTGG CCCTGGTTGA TTTCTCTTCT GAATAGTTTC 
CATCCCCATG AAGAGGACCT CTCAAGTATT AGTGCGACAC CACTTCCAGA GGAGTTTGAA 
TTACAAGGAT TTTTGGCATT GAGACCTTCT TTCAGGAACT TGGATTTTTC CAAAGGTCAC 
CAGGGTATTA CAGGGGACAA AGAAGGCCAG CAACGACGAA TACGACAGCA ACGCTTGATC 
TCTATAGGCA AATGGATTGC TGATAATCAG CCAAGGCTGA TTCAGTGTGA AAATGAGGTA 
GGGAAATTGT TGTTTATCAC AGAAATCCCA GAATTAATAC TGGAAGACCC CAGTGAAGCC 
AAAGAGAACC TCATTCTGCA AGAAACATCT GTGATAGAGT CGCTGGCTGC AGATGGGAGC 
CCAGGGCTAA AATCAGTGCT ATCTACAAGC CGAAATTTAA GCAACAACTG TGACACAGGA 
GAGAAGCCAG TGGTTACCTT CAAAGAAAAC ATTAAGACAC GAGAAGTGAA CAGAGACCAA 
GGAAGAAGTT TTCCTCCCAA AGAGGTGAGA AGGGACTATA GCAAAGGAAT AACTGTAACT 
AAGAATGATG GAAAGAAGGA CAACAACAAG AGGAAAACTG AAACCAAGAA ATGCACCTTA 
GAAAAGTTAC AGGAAACAGG AAAGCAGAAT GTGGCAGTGC AGGTAAAATC CCAGACAGAA 
CTAAGAAAGA CTCCAGTGTC TGAAGCCAGA AAAACACCTG TAACTCAAAC CCCAACTCAA 
GCAAGTAACT CCCAGTTCAT CCCCATTCAT CACCCTGGAG CCTTCCCTCC TCTTCCCAGC 
AGGCCAGGGT TTCCGCCCCC AACATATGTT ATCCCCCCGC CTGTGGCATT TTCTATGGGC 
TCAGGTTACA CCTTCCCAGC TGGTGTTTCT GTCCCAGGAA CCTTTCTTCA GCCTACAGCT 
CACTCTCCAG CAGGAAACCA GGTGCAAGCT GGGAAACAGT CCCACATTCC TTACAGCCAG 
CAACGGCCCT CTGGACCAGG GCCAATGAAC CAGGGACCTC AACAATCACA GCCACCTTCC 
CAGCAACCCC TTACATCTTT ACCAGCTCAG CCAACAGCAC AGTCTACAAG CCAGCTGCAG 
GTTCAAGCTC TAACTCAGCA ACAACAATCC CCTACAAAAG CTGTGCCGGC TTTGGGGAAA 
AGCCCGCCTC ACCACTCTGG ATTCCAGCAG TATCAACAGG CAGATGCCTC CAAACAGCTG 
TGGAATCCCC CTCAGGTTCA AGGCCCATTA GGGAAAATTA TGCCTGTGAA ACAGCCCTAC 
TACCTTCAGA CCCAAGACCC CATAAAACTG TTTGAGCCGT CATTGCAACC TCCTGTAATG 
CAGCAGCAGC CTCTAGAAAA AAAAATGAAG CCTTTTCCCA TGGAGCCATA TAACCATAAT 
CCCTCAGAAG TCAAGGTCCC AGAATTCTAC TGGGATTCTT CCTACAGCAT GGCTGATAAC 
AGATCTGTAA TGGCACAGCA AGCAAACATA GACCGCAGGG GCAAACGGTC ACCAGGAGTC 
TTCCGTCCAG AGCAGGATCC TGTACCCAGA ATGCCGTTTG AGAAATCCTT ATTGGAGAAG 
CCCTCAGAGC TCATGTCACA TTCATCCTCT TTCCTGTCCC TCACCGGATT CTCTCTCAAT 
CAGGAAAGAT ACCCAAATAA TAGTATGTTC AATGAGGTAT ATGGGAAAAA CCTGACATCC 
AGCTCCAAAG CAGAACTCAG TCCCTCAATG GCCCCCCAGG AAACATCTCT GTATTCCCTT 
TTTGAAGGGA CTCCGTGGTC TCCATCACTT CCTGCCAGTT CAGATCATTC AACACCAGCC 
AGCCAGTCTC CTCATTCCTC TAACCCAAGC AGCCTACCCA GCTCTCCTCC AACACACAAC 
CATAATTCTG TTCCATTCTC CAATTTTGGA CCCATTGGGA CTCCAGATAA CAGGGATAGA 
AGGACTGCAG ATCGGTGGAA AACTGATAAG CCAGCCATGG GTGGGTTTGG CATTGATTAT 
CTCTCAGCAA CGTCATCCTC TGAGAGCAGT TGGCATCAGG CCAGCACTCC GAGTGGCACC 
TGGACAGGCC ATGGCCCTTC CATGGAGGAT TCCTCTGCTG TCCTCATGGA AAGCCTAAAG 
TCTATCTGGT CCAGTTCCAT GATGCATCCT GGACCTTCTG CTCTGGAGCA GCTGTTAATG 
CAGCAGAAGC AGAAACAGCA ACGGGGACAA GGCACCATGA ACCCTCCACA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG7 ( NM_173156.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_775179.1 Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu07205
Clone ID Related Accession (Same CDS sequence)	NM_173156.2 , NM_173156.3
Accession Version	NM_173156.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3414bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-29
Organism	Homo sapiens(human)
Product	protein SMG7 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB223038.1, AB085674.1, DC380923.1, AY386363.1 and CN479993.1. On Jun 2, 2019 this sequence version replaced NM_173156.2. Transcript Variant: This variant (1) encodes isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7346977.2306630.1, SRR7346977.2233726.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCCTGC AGAGCGCGCA GTACCTCCGG CAGGCAGAAG TCCTGAAGGC TGACATGACA 
GATTCTAAGC TGGGTCCAGC TGAAGTCTGG ACATCCAGGC AGGCTCTGCA GGACCTGTAC 
CAGAAAATGC TAGTTACCGA TTTGGAATAC GCTTTAGACA AGAAAGTAGA ACAGGATCTC 
TGGAATCACG CCTTTAAGAA TCAGATCACA ACACTGCAAG GCCAGGCAAA GAATCGAGCA 
AATCCGAATC GGAGTGAAGT TCAGGCAAAC CTTTCTCTGT TCCTAGAGGC AGCTAGTGGC 
TTCTATACTC AGTTATTACA AGAACTGTGT ACAGTATTTA ATGTAGATTT ACCATGCCGT 
GTGAAGTCTT CCCAATTGGG AATTATCAGC AATAAACAGA CGCATACCAG CGCCATAGTG 
AAGCCACAGT CTAGCTCCTG TTCCTATATC TGCCAGCACT GCCTCGTCCA CCTTGGAGAC 
ATTGCTCGAT ACAGAAACCA GACCAGCCAG GCAGAGTCCT ACTATAGGCA TGCAGCTCAG 
CTTGTCCCCT CCAATGGTCA GCCTTATAAT CAGTTGGCTA TCTTAGCTTC TTCCAAAGGA 
GACCATCTGA CCACAATTTT CTACTACTGC AGAAGCATTG CTGTGAAGTT CCCTTTCCCA 
GCTGCCTCCA CTAATCTGCA AAAAGCACTT TCTAAAGCAC TGGAAAGCCG AGATGAGGTG 
AAAACCAAGT GGGGTGTTTC TGACTTCATC AAGGCCTTTA TTAAATTCCA CGGTCATGTG 
TACCTGAGTA AGAGCTTGGA AAAGTTGAGC CCTCTTCGAG AGAAATTGGA AGAACAGTTT 
AAGAGGCTGC TATTCCAAAA AGCTTTCAAC TCTCAGCAGT TAGTTCATGT CACTGTCATT 
AACCTGTTTC AACTTCATCA CCTTCGTGAC TTTAGCAATG AAACCGAGCA GCACACTTAT 
AGCCAAGATG AGCAGCTATG TTGGACACAG TTGCTGGCCC TCTTTATGTC TTTTCTTGGC 
ATCCTGTGCA AGTGTCCTCT ACAGAATGAG TCTCAGGAGG AGTCCTACAA TGCCTATCCT 
CTTCCAGCAG TCAAGGTCTC CATGGACTGG CTAAGACTCA GACCCAGGGT CTTTCAGGAG 
GCAGTGGTGG ATGAAAGACA GTACATTTGG CCCTGGTTGA TTTCTCTTCT GAATAGTTTC 
CATCCCCATG AAGAGGACCT CTCAAGTATT AGTGCGACAC CACTTCCAGA GGAGTTTGAA 
TTACAAGGAT TTTTGGCATT GAGACCTTCT TTCAGGAACT TGGATTTTTC CAAAGGTCAC 
CAGGGTATTA CAGGGGACAA AGAAGGCCAG CAACGACGAA TACGACAGCA ACGCTTGATC 
TCTATAGGCA AATGGATTGC TGATAATCAG CCAAGGCTGA TTCAGTGTGA AAATGAGGTA 
GGGAAATTGT TGTTTATCAC AGAAATCCCA GAATTAATAC TGGAAGACCC CAGTGAAGCC 
AAAGAGAACC TCATTCTGCA AGAAACATCT GTGATAGAGT CGCTGGCTGC AGATGGGAGC 
CCAGGGCTAA AATCAGTGCT ATCTACAAGC CGAAATTTAA GCAACAACTG TGACACAGGA 
GAGAAGCCAG TGGTTACCTT CAAAGAAAAC ATTAAGACAC GAGAAGTGAA CAGAGACCAA 
GGAAGAAGTT TTCCTCCCAA AGAGGTGAGA AGGGACTATA GCAAAGGAAT AACTGTAACT 
AAGAATGATG GAAAGAAGGA CAACAACAAG AGGAAAACTG AAACCAAGAA ATGCACCTTA 
GAAAAGTTAC AGGAAACAGG AAAGCAGAAT GTGGCAGTGC AGGTAAAATC CCAGACAGAA 
CTAAGAAAGA CTCCAGTGTC TGAAGCCAGA AAAACACCTG TAACTCAAAC CCCAACTCAA 
GCAAGTAACT CCCAGTTCAT CCCCATTCAT CACCCTGGAG CCTTCCCTCC TCTTCCCAGC 
AGGCCAGGGT TTCCGCCCCC AACATATGTT ATCCCCCCGC CTGTGGCATT TTCTATGGGC 
TCAGGTTACA CCTTCCCAGC TGGTGTTTCT GTCCCAGGAA CCTTTCTTCA GCCTACAGCT 
CACTCTCCAG CAGGAAACCA GGTGCAAGCT GGGAAACAGT CCCACATTCC TTACAGCCAG 
CAACGGCCCT CTGGACCAGG GCCAATGAAC CAGGGACCTC AACAATCACA GCCACCTTCC 
CAGCAACCCC TTACATCTTT ACCAGCTCAG CCAACAGCAC AGTCTACAAG CCAGCTGCAG 
GTTCAAGCTC TAACTCAGCA ACAACAATCC CCTACAAAAG CTGTGCCGGC TTTGGGGAAA 
AGCCCGCCTC ACCACTCTGG ATTCCAGCAG TATCAACAGG CAGATGCCTC CAAACAGCTG 
TGGAATCCCC CTCAGGTTCA AGGCCCATTA GGGAAAATTA TGCCTGTGAA ACAGCCCTAC 
TACCTTCAGA CCCAAGACCC CATAAAACTG TTTGAGCCGT CATTGCAACC TCCTGTAATG 
CAGCAGCAGC CTCTAGAAAA AAAAATGAAG CCTTTTCCCA TGGAGCCATA TAACCATAAT 
CCCTCAGAAG TCAAGGTCCC AGAATTCTAC TGGGATTCTT CCTACAGCAT GGCTGATAAC 
AGATCTGTAA TGGCACAGCA AGCAAACATA GACCGCAGGG GCAAACGGTC ACCAGGAGTC 
TTCCGTCCAG AGCAGGATCC TGTACCCAGA ATGCCGTTTG AGAAATCCTT ATTGGAGAAG 
CCCTCAGAGC TCATGTCACA TTCATCCTCT TTCCTGTCCC TCACCGGATT CTCTCTCAAT 
CAGGAAAGAT ACCCAAATAA TAGTATGTTC AATGAGGTAT ATGGGAAAAA CCTGACATCC 
AGCTCCAAAG CAGAACTCAG TCCCTCAATG GCCCCCCAGG AAACATCTCT GTATTCCCTT 
TTTGAAGGGA CTCCGTGGTC TCCATCACTT CCTGCCAGTT CAGATCATTC AACACCAGCC 
AGCCAGTCTC CTCATTCCTC TAACCCAAGC AGCCTACCCA GCTCTCCTCC AACACACAAC 
CATAATTCTG TTCCATTCTC CAATTTTGGA CCCATTGGGA CTCCAGATAA CAGGGATAGA 
AGGACTGCAG ATCGGTGGAA AACTGATAAG CCAGCCATGG GTGGGTTTGG CATTGATTAT 
CTCTCAGCAA CGTCATCCTC TGAGAGCAGT TGGCATCAGG CCAGCACTCC GAGTGGCACC 
TGGACAGGCC ATGGCCCTTC CATGGAGGAT TCCTCTGCTG TCCTCATGGA AAGCCTAAAG 
TCTATCTGGT CCAGTTCCAT GATGCATCCT GGACCTTCTG CTCTGGAGCA GCTGTTAATG 
CAGCAGAAGC AGAAACAGCA ACGGGGACAA GGCACCATGA ACCCTCCACA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_775179.1
CDS	123..3536
Translation	MSLQSAQYLRQAEVLKADMTDSKLGPAEVWTSRQALQDLYQKMLVTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRANPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQTHTSAIVKPQSSSCSYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNLQKALSKALESRDEVKTKWGVSDFIKAFIKFHGHVYLSKSLEKLSPLREKLEEQFKRLLFQKAFNSQQLVHVTVINLFQLHHLRDFSNETEQHTYSQDEQLCWTQLLALFMSFLGILCKCPLQNESQEESYNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPHEEDLSSISATPLPEEFELQGFLALRPSFRNLDFSKGHQGITGDKEGQQRRIRQQRLISIGKWIADNQPRLIQCENEVGKLLFITEIPELILEDPSEAKENLILQETSVIESLAADGSPGLKSVLSTSRNLSNNCDTGEKPVVTFKENIKTREVNRDQGRSFPPKEVRRDYSKGITVTKNDGKKDNNKRKTETKKCTLEKLQETGKQNVAVQVKSQTELRKTPVSEARKTPVTQTPTQASNSQFIPIHHPGAFPPLPSRPGFPPPTYVIPPPVAFSMGSGYTFPAGVSVPGTFLQPTAHSPAGNQVQAGKQSHIPYSQQRPSGPGPMNQGPQQSQPPSQQPLTSLPAQPTAQSTSQLQVQALTQQQQSPTKAVPALGKSPPHHSGFQQYQQADASKQLWNPPQVQGPLGKIMPVKQPYYLQTQDPIKLFEPSLQPPVMQQQPLEKKMKPFPMEPYNHNPSEVKVPEFYWDSSYSMADNRSVMAQQANIDRRGKRSPGVFRPEQDPVPRMPFEKSLLEKPSELMSHSSSFLSLTGFSLNQERYPNNSMFNEVYGKNLTSSSKAELSPSMAPQETSLYSLFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSVPFSNFGPIGTPDNRDRRTADRWKTDKPAMGGFGIDYLSATSSSESSWHQASTPSGTWTGHGPSMEDSSAVLMESLKSIWSSSMMHPGPSALEQLLMQQKQKQQRGQGTMNPPH

Target ORF information:

RefSeq Version	NM_173156.3
Organism	Homo sapiens(human)
Definition	Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_173156.3

ORF Insert Sequence:

ATGAGCCTGC AGAGCGCGCA GTACCTCCGG CAGGCAGAAG TCCTGAAGGC TGACATGACA 
GATTCTAAGC TGGGTCCAGC TGAAGTCTGG ACATCCAGGC AGGCTCTGCA GGACCTGTAC 
CAGAAAATGC TAGTTACCGA TTTGGAATAC GCTTTAGACA AGAAAGTAGA ACAGGATCTC 
TGGAATCACG CCTTTAAGAA TCAGATCACA ACACTGCAAG GCCAGGCAAA GAATCGAGCA 
AATCCGAATC GGAGTGAAGT TCAGGCAAAC CTTTCTCTGT TCCTAGAGGC AGCTAGTGGC 
TTCTATACTC AGTTATTACA AGAACTGTGT ACAGTATTTA ATGTAGATTT ACCATGCCGT 
GTGAAGTCTT CCCAATTGGG AATTATCAGC AATAAACAGA CGCATACCAG CGCCATAGTG 
AAGCCACAGT CTAGCTCCTG TTCCTATATC TGCCAGCACT GCCTCGTCCA CCTTGGAGAC 
ATTGCTCGAT ACAGAAACCA GACCAGCCAG GCAGAGTCCT ACTATAGGCA TGCAGCTCAG 
CTTGTCCCCT CCAATGGTCA GCCTTATAAT CAGTTGGCTA TCTTAGCTTC TTCCAAAGGA 
GACCATCTGA CCACAATTTT CTACTACTGC AGAAGCATTG CTGTGAAGTT CCCTTTCCCA 
GCTGCCTCCA CTAATCTGCA AAAAGCACTT TCTAAAGCAC TGGAAAGCCG AGATGAGGTG 
AAAACCAAGT GGGGTGTTTC TGACTTCATC AAGGCCTTTA TTAAATTCCA CGGTCATGTG 
TACCTGAGTA AGAGCTTGGA AAAGTTGAGC CCTCTTCGAG AGAAATTGGA AGAACAGTTT 
AAGAGGCTGC TATTCCAAAA AGCTTTCAAC TCTCAGCAGT TAGTTCATGT CACTGTCATT 
AACCTGTTTC AACTTCATCA CCTTCGTGAC TTTAGCAATG AAACCGAGCA GCACACTTAT 
AGCCAAGATG AGCAGCTATG TTGGACACAG TTGCTGGCCC TCTTTATGTC TTTTCTTGGC 
ATCCTGTGCA AGTGTCCTCT ACAGAATGAG TCTCAGGAGG AGTCCTACAA TGCCTATCCT 
CTTCCAGCAG TCAAGGTCTC CATGGACTGG CTAAGACTCA GACCCAGGGT CTTTCAGGAG 
GCAGTGGTGG ATGAAAGACA GTACATTTGG CCCTGGTTGA TTTCTCTTCT GAATAGTTTC 
CATCCCCATG AAGAGGACCT CTCAAGTATT AGTGCGACAC CACTTCCAGA GGAGTTTGAA 
TTACAAGGAT TTTTGGCATT GAGACCTTCT TTCAGGAACT TGGATTTTTC CAAAGGTCAC 
CAGGGTATTA CAGGGGACAA AGAAGGCCAG CAACGACGAA TACGACAGCA ACGCTTGATC 
TCTATAGGCA AATGGATTGC TGATAATCAG CCAAGGCTGA TTCAGTGTGA AAATGAGGTA 
GGGAAATTGT TGTTTATCAC AGAAATCCCA GAATTAATAC TGGAAGACCC CAGTGAAGCC 
AAAGAGAACC TCATTCTGCA AGAAACATCT GTGATAGAGT CGCTGGCTGC AGATGGGAGC 
CCAGGGCTAA AATCAGTGCT ATCTACAAGC CGAAATTTAA GCAACAACTG TGACACAGGA 
GAGAAGCCAG TGGTTACCTT CAAAGAAAAC ATTAAGACAC GAGAAGTGAA CAGAGACCAA 
GGAAGAAGTT TTCCTCCCAA AGAGGTGAGA AGGGACTATA GCAAAGGAAT AACTGTAACT 
AAGAATGATG GAAAGAAGGA CAACAACAAG AGGAAAACTG AAACCAAGAA ATGCACCTTA 
GAAAAGTTAC AGGAAACAGG AAAGCAGAAT GTGGCAGTGC AGGTAAAATC CCAGACAGAA 
CTAAGAAAGA CTCCAGTGTC TGAAGCCAGA AAAACACCTG TAACTCAAAC CCCAACTCAA 
GCAAGTAACT CCCAGTTCAT CCCCATTCAT CACCCTGGAG CCTTCCCTCC TCTTCCCAGC 
AGGCCAGGGT TTCCGCCCCC AACATATGTT ATCCCCCCGC CTGTGGCATT TTCTATGGGC 
TCAGGTTACA CCTTCCCAGC TGGTGTTTCT GTCCCAGGAA CCTTTCTTCA GCCTACAGCT 
CACTCTCCAG CAGGAAACCA GGTGCAAGCT GGGAAACAGT CCCACATTCC TTACAGCCAG 
CAACGGCCCT CTGGACCAGG GCCAATGAAC CAGGGACCTC AACAATCACA GCCACCTTCC 
CAGCAACCCC TTACATCTTT ACCAGCTCAG CCAACAGCAC AGTCTACAAG CCAGCTGCAG 
GTTCAAGCTC TAACTCAGCA ACAACAATCC CCTACAAAAG CTGTGCCGGC TTTGGGGAAA 
AGCCCGCCTC ACCACTCTGG ATTCCAGCAG TATCAACAGG CAGATGCCTC CAAACAGCTG 
TGGAATCCCC CTCAGGTTCA AGGCCCATTA GGGAAAATTA TGCCTGTGAA ACAGCCCTAC 
TACCTTCAGA CCCAAGACCC CATAAAACTG TTTGAGCCGT CATTGCAACC TCCTGTAATG 
CAGCAGCAGC CTCTAGAAAA AAAAATGAAG CCTTTTCCCA TGGAGCCATA TAACCATAAT 
CCCTCAGAAG TCAAGGTCCC AGAATTCTAC TGGGATTCTT CCTACAGCAT GGCTGATAAC 
AGATCTGTAA TGGCACAGCA AGCAAACATA GACCGCAGGG GCAAACGGTC ACCAGGAGTC 
TTCCGTCCAG AGCAGGATCC TGTACCCAGA ATGCCGTTTG AGAAATCCTT ATTGGAGAAG 
CCCTCAGAGC TCATGTCACA TTCATCCTCT TTCCTGTCCC TCACCGGATT CTCTCTCAAT 
CAGGAAAGAT ACCCAAATAA TAGTATGTTC AATGAGGTAT ATGGGAAAAA CCTGACATCC 
AGCTCCAAAG CAGAACTCAG TCCCTCAATG GCCCCCCAGG AAACATCTCT GTATTCCCTT 
TTTGAAGGGA CTCCGTGGTC TCCATCACTT CCTGCCAGTT CAGATCATTC AACACCAGCC 
AGCCAGTCTC CTCATTCCTC TAACCCAAGC AGCCTACCCA GCTCTCCTCC AACACACAAC 
CATAATTCTG TTCCATTCTC CAATTTTGGA CCCATTGGGA CTCCAGATAA CAGGGATAGA 
AGGACTGCAG ATCGGTGGAA AACTGATAAG CCAGCCATGG GTGGGTTTGG CATTGATTAT 
CTCTCAGCAA CGTCATCCTC TGAGAGCAGT TGGCATCAGG CCAGCACTCC GAGTGGCACC 
TGGACAGGCC ATGGCCCTTC CATGGAGGAT TCCTCTGCTG TCCTCATGGA AAGCCTAAAG 
TCTATCTGGT CCAGTTCCAT GATGCATCCT GGACCTTCTG CTCTGGAGCA GCTGTTAATG 
CAGCAGAAGC AGAAACAGCA ACGGGGACAA GGCACCATGA ACCCTCCACA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG7 ( NM_201569.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_963863.2 Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 4, mRNA.

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CloneID	OHu22107
Clone ID Related Accession (Same CDS sequence)	NM_201569.2 , NM_201569.3
Accession Version	NM_201569.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-27
Organism	Homo sapiens(human)
Product	protein SMG7 isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB223038.1, BC036381.1, AB085674.1, AK299178.1, AL137800.12 and CN479993.1. This sequence is a reference standard in the RefSeqGene project. On Mar 23, 2010 this sequence version replaced NM_201569.1. Transcript Variant: This variant (4) has multiple differences in the coding region compared to variant 1, one of which results in a translational frameshift. The resulting protein (isoform 4) has a distinct C-terminus and is longer than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC036381.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCCTGC AGAGCGCGCA GTACCTCCGG CAGGCAGAAG TCCTGAAGGC TGACATGACA 
GATTCTAAGC TGGGTCCAGC TGAAGTCTGG ACATCCAGGC AGGCTCTGCA GGACCTGTAC 
CAGAAAATGC TAGTTACCGA TTTGGAATAC GCTTTAGACA AGAAAGTAGA ACAGGATCTC 
TGGAATCACG CCTTTAAGAA TCAGATCACA ACACTGCAAG GCCAGGCAAA GAATCGAGCA 
AATCCGAATC GGAGTGAAGT TCAGGCAAAC CTTTCTCTGT TCCTAGAGGC AGCTAGTGGC 
TTCTATACTC AGTTATTACA AGAACTGTGT ACAGTATTTA ATGTAGATTT ACCATGCCGT 
GTGAAGTCTT CCCAATTGGG AATTATCAGC AATAAACAGA CGCATACCAG CGCCATAGTG 
AAGCCACAGT CTAGCTCCTG TTCCTATATC TGCCAGCACT GCCTCGTCCA CCTTGGAGAC 
ATTGCTCGAT ACAGAAACCA GACCAGCCAG GCAGAGTCCT ACTATAGGCA TGCAGCTCAG 
CTTGTCCCCT CCAATGGTCA GCCTTATAAT CAGTTGGCTA TCTTAGCTTC TTCCAAAGGA 
GACCATCTGA CCACAATTTT CTACTACTGC AGAAGCATTG CTGTGAAGTT CCCTTTCCCA 
GCTGCCTCCA CTAATCTGCA AAAAGCACTT TCTAAAGCAC TGGAAAGCCG AGATGAGGTG 
AAAACCAAGT GGGGTGTTTC TGACTTCATC AAGGCCTTTA TTAAATTCCA CGGTCATGTG 
TACCTGAGTA AGAGCTTGGA AAAGTTGAGC CCTCTTCGAG AGAAATTGGA AGAACAGTTT 
AAGAGGCTGC TATTCCAAAA AGCTTTCAAC TCTCAGCAGT TAGTTCATGT CACTGTCATT 
AACCTGTTTC AACTTCATCA CCTTCGTGAC TTTAGCAATG AAACCGAGCA GCACACTTAT 
AGCCAAGATG AGCAGCTATG TTGGACACAG TTGCTGGCCC TCTTTATGTC TTTTCTTGGC 
ATCCTGTGCA AGTGTCCTCT ACAGAATGAG TCTCAGGAGG AGTCCTACAA TGCCTATCCT 
CTTCCAGCAG TCAAGGTCTC CATGGACTGG CTAAGACTCA GACCCAGGGT CTTTCAGGAG 
GCAGTGGTGG ATGAAAGACA GTACATTTGG CCCTGGTTGA TTTCTCTTCT GAATAGTTTC 
CATCCCCATG AAGAGGACCT CTCAAGTATT AGTGCGACAC CACTTCCAGA GGAGTTTGAA 
TTACAAGGAT TTTTGGCATT GAGACCTTCT TTCAGGAACT TGGATTTTTC CAAAGGTCAC 
CAGGGTATTA CAGGGGACAA AGAAGGCCAG CAACGACGAA TACGACAGCA ACGCTTGATC 
TCTATAGGCA AATGGATTGC TGATAATCAG CCAAGGCTGA TTCAGTGTGA AAATGAGGTA 
GGGAAATTGT TGTTTATCAC AGAAATCCCA GAATTAATAC TGGAAGACCC CAGTGAAGCC 
AAAGAGAACC TCATTCTGCA AGAAACATCT GTGATAGAGT CGCTGGCTGC AGATGGGAGC 
CCAGGGCTAA AATCAGTGCT ATCTACAAGC CGAAATTTAA GCAACAACTG TGACACAGGA 
GAGAAGCCAG TGGTTACCTT CAAAGAAAAC ATTAAGACAC GAGAAGTGAA CAGAGACCAA 
GGAAGAAGTT TTCCTCCCAA AGAGGTAAAA TCCCAGACAG AACTAAGAAA GACTCCAGTG 
TCTGAAGCCA GAAAAACACC TGTAACTCAA ACCCCAACTC AAGCAAGTAA CTCCCAGTTC 
ATCCCCATTC ATCACCCTGG AGCCTTCCCT CCTCTTCCCA GCAGGCCAGG GTTTCCGCCC 
CCAACATATG TTATCCCCCC GCCTGTGGCA TTTTCTATGG GCTCAGGTTA CACCTTCCCA 
GCTGGTGTTT CTGTCCCAGG AACCTTTCTT CAGCCTACAG CTCACTCTCC AGCAGGAAAC 
CAGGTGCAAG CTGGGAAACA GTCCCACATT CCTTACAGCC AGCAACGGCC CTCTGGACCA 
GGGCCAATGA ACCAGGGACC TCAACAATCA CAGCCACCTT CCCAGCAACC CCTTACATCT 
TTACCAGCTC AGCCAACAGC ACAGTCTACA AGCCAGCTGC AGGTTCAAGC TCTAACTCAG 
CAACAACAAT CCCCTACAAA AGCTGTGCCG GCTTTGGGGA AAAGCCCGCC TCACCACTCT 
GGATTCCAGC AGTATCAACA GGCAGATGCC TCCAAACAGC TGTGGAATCC CCCTCAGGTT 
CAAGGCCCAT TAGGGAAAAT TATGCCTGTG AAACAGCCCT ACTACCTTCA GACCCAAGAC 
CCCATAAAAC TGTTTGAGCC GTCATTGCAA CCTCCTGTAA TGCAGCAGCA GCCTCTAGAA 
AAAAAAATGA AGCCTTTTCC CATGGAGCCA TATAACCATA ATCCCTCAGA AGTCAAGGTC 
CCAGAATTCT ACTGGGATTC TTCCTACAGC ATGGCTGATA ACAGATCTGT AATGGCACAG 
CAAGCAAACA TAGACCGCAG GGGCAAACGG TCACCAGGAG TCTTCCGTCC AGAGCAGGAT 
CCTGTACCCA GAATGCCGTT TGAGGACCCC AAGAGCTCCC CTCTGCTTCC TCCGGACCTG 
TTAAAGAGTC TGGCTGCCTT GGAGGAAGAG GAAGAGCTGA TTTTTTCTAA CCCTCCTGAT 
CTTTACCCGG CTCTGCTGGG GCCTCTCGCC TCTCTTCCTG GACGAAGCCT TTTTAAATCC 
TTATTGGAGA AGCCCTCAGA GCTCATGTCA CATTCATCCT CTTTCCTGTC CCTCACCGGA 
TTCTCTCTCA ATCAGGAAAG ATACCCAAAT AATAGTATGT TCAATGAGGT ATATGGGAAA 
AACCTGACAT CCAGCTCCAA AGCAGAACTC AGTCCCTCAA TGGCCCCCCA GGAAACATCT 
CTGTATTCCC TTTTTGAAGG GACTCCGTGG TCTCCATCAC TTCCTGCCAG TTCAGATCAT 
TCAACACCAG CCAGCCAGTC TCCTCATTCC TCTAACCCAA GCAGCCTACC CAGCTCTCCT 
CCAACACACA ACCATAATTC TGTTCCATTC TCCAATTTTG GACCCATTGG GACTCCAGAT 
AACAGGGATA GAAGGACTGC AGATCGGTGG AAAACTGATA AGCCAGCCAT GGGTGGGTTT 
GGCATTGATT ATCTCTCAGC AACGTCATCC TCTGAGAGCA GTTGGCATCA GGCCAGCACT 
CCGAGTGGCA CCTGGACAGG CCATGGCCCT TCCATGGAGG ATTCCTCTGC TGTCCTCATG 
GAAAGCCTAA AGAAGCAACA GCATGGGGTC CAGCAGTTGG GGCCCAAAAG ACAGTCTGAA 
GAGGAAGGAA GCAGCAGTAT CTGCGTAGCC CACAGAGGGC CCAGGCCCCT GCCCAGCTGC 
AGTCTCCCAG CCTCCACTTT CAGAGTGAAA TTCAAGGCAG CACGGACATG TGCCCATCAG 
GCACAGAAGA AAACACGACG TCGTCCATTT TGGAAGAGAC GAAAGAAAGG AAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_963863.2
CDS	251..3787
Translation	MSLQSAQYLRQAEVLKADMTDSKLGPAEVWTSRQALQDLYQKMLVTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRANPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQTHTSAIVKPQSSSCSYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNLQKALSKALESRDEVKTKWGVSDFIKAFIKFHGHVYLSKSLEKLSPLREKLEEQFKRLLFQKAFNSQQLVHVTVINLFQLHHLRDFSNETEQHTYSQDEQLCWTQLLALFMSFLGILCKCPLQNESQEESYNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPHEEDLSSISATPLPEEFELQGFLALRPSFRNLDFSKGHQGITGDKEGQQRRIRQQRLISIGKWIADNQPRLIQCENEVGKLLFITEIPELILEDPSEAKENLILQETSVIESLAADGSPGLKSVLSTSRNLSNNCDTGEKPVVTFKENIKTREVNRDQGRSFPPKEVKSQTELRKTPVSEARKTPVTQTPTQASNSQFIPIHHPGAFPPLPSRPGFPPPTYVIPPPVAFSMGSGYTFPAGVSVPGTFLQPTAHSPAGNQVQAGKQSHIPYSQQRPSGPGPMNQGPQQSQPPSQQPLTSLPAQPTAQSTSQLQVQALTQQQQSPTKAVPALGKSPPHHSGFQQYQQADASKQLWNPPQVQGPLGKIMPVKQPYYLQTQDPIKLFEPSLQPPVMQQQPLEKKMKPFPMEPYNHNPSEVKVPEFYWDSSYSMADNRSVMAQQANIDRRGKRSPGVFRPEQDPVPRMPFEDPKSSPLLPPDLLKSLAALEEEEELIFSNPPDLYPALLGPLASLPGRSLFKSLLEKPSELMSHSSSFLSLTGFSLNQERYPNNSMFNEVYGKNLTSSSKAELSPSMAPQETSLYSLFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSVPFSNFGPIGTPDNRDRRTADRWKTDKPAMGGFGIDYLSATSSSESSWHQASTPSGTWTGHGPSMEDSSAVLMESLKKQQHGVQQLGPKRQSEEEGSSSICVAHRGPRPLPSCSLPASTFRVKFKAARTCAHQAQKKTRRRPFWKRRKKGK

Target ORF information:

RefSeq Version	NM_201569.2
Organism	Homo sapiens(human)
Definition	Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_201569.2

ORF Insert Sequence:

ATGAGCCTGC AGAGCGCGCA GTACCTCCGG CAGGCAGAAG TCCTGAAGGC TGACATGACA 
GATTCTAAGC TGGGTCCAGC TGAAGTCTGG ACATCCAGGC AGGCTCTGCA GGACCTGTAC 
CAGAAAATGC TAGTTACCGA TTTGGAATAC GCTTTAGACA AGAAAGTAGA ACAGGATCTC 
TGGAATCACG CCTTTAAGAA TCAGATCACA ACACTGCAAG GCCAGGCAAA GAATCGAGCA 
AATCCGAATC GGAGTGAAGT TCAGGCAAAC CTTTCTCTGT TCCTAGAGGC AGCTAGTGGC 
TTCTATACTC AGTTATTACA AGAACTGTGT ACAGTATTTA ATGTAGATTT ACCATGCCGT 
GTGAAGTCTT CCCAATTGGG AATTATCAGC AATAAACAGA CGCATACCAG CGCCATAGTG 
AAGCCACAGT CTAGCTCCTG TTCCTATATC TGCCAGCACT GCCTCGTCCA CCTTGGAGAC 
ATTGCTCGAT ACAGAAACCA GACCAGCCAG GCAGAGTCCT ACTATAGGCA TGCAGCTCAG 
CTTGTCCCCT CCAATGGTCA GCCTTATAAT CAGTTGGCTA TCTTAGCTTC TTCCAAAGGA 
GACCATCTGA CCACAATTTT CTACTACTGC AGAAGCATTG CTGTGAAGTT CCCTTTCCCA 
GCTGCCTCCA CTAATCTGCA AAAAGCACTT TCTAAAGCAC TGGAAAGCCG AGATGAGGTG 
AAAACCAAGT GGGGTGTTTC TGACTTCATC AAGGCCTTTA TTAAATTCCA CGGTCATGTG 
TACCTGAGTA AGAGCTTGGA AAAGTTGAGC CCTCTTCGAG AGAAATTGGA AGAACAGTTT 
AAGAGGCTGC TATTCCAAAA AGCTTTCAAC TCTCAGCAGT TAGTTCATGT CACTGTCATT 
AACCTGTTTC AACTTCATCA CCTTCGTGAC TTTAGCAATG AAACCGAGCA GCACACTTAT 
AGCCAAGATG AGCAGCTATG TTGGACACAG TTGCTGGCCC TCTTTATGTC TTTTCTTGGC 
ATCCTGTGCA AGTGTCCTCT ACAGAATGAG TCTCAGGAGG AGTCCTACAA TGCCTATCCT 
CTTCCAGCAG TCAAGGTCTC CATGGACTGG CTAAGACTCA GACCCAGGGT CTTTCAGGAG 
GCAGTGGTGG ATGAAAGACA GTACATTTGG CCCTGGTTGA TTTCTCTTCT GAATAGTTTC 
CATCCCCATG AAGAGGACCT CTCAAGTATT AGTGCGACAC CACTTCCAGA GGAGTTTGAA 
TTACAAGGAT TTTTGGCATT GAGACCTTCT TTCAGGAACT TGGATTTTTC CAAAGGTCAC 
CAGGGTATTA CAGGGGACAA AGAAGGCCAG CAACGACGAA TACGACAGCA ACGCTTGATC 
TCTATAGGCA AATGGATTGC TGATAATCAG CCAAGGCTGA TTCAGTGTGA AAATGAGGTA 
GGGAAATTGT TGTTTATCAC AGAAATCCCA GAATTAATAC TGGAAGACCC CAGTGAAGCC 
AAAGAGAACC TCATTCTGCA AGAAACATCT GTGATAGAGT CGCTGGCTGC AGATGGGAGC 
CCAGGGCTAA AATCAGTGCT ATCTACAAGC CGAAATTTAA GCAACAACTG TGACACAGGA 
GAGAAGCCAG TGGTTACCTT CAAAGAAAAC ATTAAGACAC GAGAAGTGAA CAGAGACCAA 
GGAAGAAGTT TTCCTCCCAA AGAGGTAAAA TCCCAGACAG AACTAAGAAA GACTCCAGTG 
TCTGAAGCCA GAAAAACACC TGTAACTCAA ACCCCAACTC AAGCAAGTAA CTCCCAGTTC 
ATCCCCATTC ATCACCCTGG AGCCTTCCCT CCTCTTCCCA GCAGGCCAGG GTTTCCGCCC 
CCAACATATG TTATCCCCCC GCCTGTGGCA TTTTCTATGG GCTCAGGTTA CACCTTCCCA 
GCTGGTGTTT CTGTCCCAGG AACCTTTCTT CAGCCTACAG CTCACTCTCC AGCAGGAAAC 
CAGGTGCAAG CTGGGAAACA GTCCCACATT CCTTACAGCC AGCAACGGCC CTCTGGACCA 
GGGCCAATGA ACCAGGGACC TCAACAATCA CAGCCACCTT CCCAGCAACC CCTTACATCT 
TTACCAGCTC AGCCAACAGC ACAGTCTACA AGCCAGCTGC AGGTTCAAGC TCTAACTCAG 
CAACAACAAT CCCCTACAAA AGCTGTGCCG GCTTTGGGGA AAAGCCCGCC TCACCACTCT 
GGATTCCAGC AGTATCAACA GGCAGATGCC TCCAAACAGC TGTGGAATCC CCCTCAGGTT 
CAAGGCCCAT TAGGGAAAAT TATGCCTGTG AAACAGCCCT ACTACCTTCA GACCCAAGAC 
CCCATAAAAC TGTTTGAGCC GTCATTGCAA CCTCCTGTAA TGCAGCAGCA GCCTCTAGAA 
AAAAAAATGA AGCCTTTTCC CATGGAGCCA TATAACCATA ATCCCTCAGA AGTCAAGGTC 
CCAGAATTCT ACTGGGATTC TTCCTACAGC ATGGCTGATA ACAGATCTGT AATGGCACAG 
CAAGCAAACA TAGACCGCAG GGGCAAACGG TCACCAGGAG TCTTCCGTCC AGAGCAGGAT 
CCTGTACCCA GAATGCCGTT TGAGGACCCC AAGAGCTCCC CTCTGCTTCC TCCGGACCTG 
TTAAAGAGTC TGGCTGCCTT GGAGGAAGAG GAAGAGCTGA TTTTTTCTAA CCCTCCTGAT 
CTTTACCCGG CTCTGCTGGG GCCTCTCGCC TCTCTTCCTG GACGAAGCCT TTTTAAATCC 
TTATTGGAGA AGCCCTCAGA GCTCATGTCA CATTCATCCT CTTTCCTGTC CCTCACCGGA 
TTCTCTCTCA ATCAGGAAAG ATACCCAAAT AATAGTATGT TCAATGAGGT ATATGGGAAA 
AACCTGACAT CCAGCTCCAA AGCAGAACTC AGTCCCTCAA TGGCCCCCCA GGAAACATCT 
CTGTATTCCC TTTTTGAAGG GACTCCGTGG TCTCCATCAC TTCCTGCCAG TTCAGATCAT 
TCAACACCAG CCAGCCAGTC TCCTCATTCC TCTAACCCAA GCAGCCTACC CAGCTCTCCT 
CCAACACACA ACCATAATTC TGTTCCATTC TCCAATTTTG GACCCATTGG GACTCCAGAT 
AACAGGGATA GAAGGACTGC AGATCGGTGG AAAACTGATA AGCCAGCCAT GGGTGGGTTT 
GGCATTGATT ATCTCTCAGC AACGTCATCC TCTGAGAGCA GTTGGCATCA GGCCAGCACT 
CCGAGTGGCA CCTGGACAGG CCATGGCCCT TCCATGGAGG ATTCCTCTGC TGTCCTCATG 
GAAAGCCTAA AGAAGCAACA GCATGGGGTC CAGCAGTTGG GGCCCAAAAG ACAGTCTGAA 
GAGGAAGGAA GCAGCAGTAT CTGCGTAGCC CACAGAGGGC CCAGGCCCCT GCCCAGCTGC 
AGTCTCCCAG CCTCCACTTT CAGAGTGAAA TTCAAGGCAG CACGGACATG TGCCCATCAG 
GCACAGAAGA AAACACGACG TCGTCCATTT TGGAAGAGAC GAAAGAAAGG AAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG7 ( NM_201569.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_963863.2 Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 4, mRNA.

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CloneID	OHu22107
Clone ID Related Accession (Same CDS sequence)	NM_201569.2 , NM_201569.3
Accession Version	NM_201569.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-28
Organism	Homo sapiens(human)
Product	protein SMG7 isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB223038.1, BC036381.1, AB085674.1, AK299178.1, AL137800.12 and CN479993.1. This sequence is a reference standard in the RefSeqGene project. On Jun 2, 2019 this sequence version replaced NM_201569.2. Transcript Variant: This variant (4) has multiple differences in the coding region compared to variant 1, one of which results in a translational frameshift. The resulting protein (isoform 4) has a distinct C-terminus and is longer than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC036381.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCCTGC AGAGCGCGCA GTACCTCCGG CAGGCAGAAG TCCTGAAGGC TGACATGACA 
GATTCTAAGC TGGGTCCAGC TGAAGTCTGG ACATCCAGGC AGGCTCTGCA GGACCTGTAC 
CAGAAAATGC TAGTTACCGA TTTGGAATAC GCTTTAGACA AGAAAGTAGA ACAGGATCTC 
TGGAATCACG CCTTTAAGAA TCAGATCACA ACACTGCAAG GCCAGGCAAA GAATCGAGCA 
AATCCGAATC GGAGTGAAGT TCAGGCAAAC CTTTCTCTGT TCCTAGAGGC AGCTAGTGGC 
TTCTATACTC AGTTATTACA AGAACTGTGT ACAGTATTTA ATGTAGATTT ACCATGCCGT 
GTGAAGTCTT CCCAATTGGG AATTATCAGC AATAAACAGA CGCATACCAG CGCCATAGTG 
AAGCCACAGT CTAGCTCCTG TTCCTATATC TGCCAGCACT GCCTCGTCCA CCTTGGAGAC 
ATTGCTCGAT ACAGAAACCA GACCAGCCAG GCAGAGTCCT ACTATAGGCA TGCAGCTCAG 
CTTGTCCCCT CCAATGGTCA GCCTTATAAT CAGTTGGCTA TCTTAGCTTC TTCCAAAGGA 
GACCATCTGA CCACAATTTT CTACTACTGC AGAAGCATTG CTGTGAAGTT CCCTTTCCCA 
GCTGCCTCCA CTAATCTGCA AAAAGCACTT TCTAAAGCAC TGGAAAGCCG AGATGAGGTG 
AAAACCAAGT GGGGTGTTTC TGACTTCATC AAGGCCTTTA TTAAATTCCA CGGTCATGTG 
TACCTGAGTA AGAGCTTGGA AAAGTTGAGC CCTCTTCGAG AGAAATTGGA AGAACAGTTT 
AAGAGGCTGC TATTCCAAAA AGCTTTCAAC TCTCAGCAGT TAGTTCATGT CACTGTCATT 
AACCTGTTTC AACTTCATCA CCTTCGTGAC TTTAGCAATG AAACCGAGCA GCACACTTAT 
AGCCAAGATG AGCAGCTATG TTGGACACAG TTGCTGGCCC TCTTTATGTC TTTTCTTGGC 
ATCCTGTGCA AGTGTCCTCT ACAGAATGAG TCTCAGGAGG AGTCCTACAA TGCCTATCCT 
CTTCCAGCAG TCAAGGTCTC CATGGACTGG CTAAGACTCA GACCCAGGGT CTTTCAGGAG 
GCAGTGGTGG ATGAAAGACA GTACATTTGG CCCTGGTTGA TTTCTCTTCT GAATAGTTTC 
CATCCCCATG AAGAGGACCT CTCAAGTATT AGTGCGACAC CACTTCCAGA GGAGTTTGAA 
TTACAAGGAT TTTTGGCATT GAGACCTTCT TTCAGGAACT TGGATTTTTC CAAAGGTCAC 
CAGGGTATTA CAGGGGACAA AGAAGGCCAG CAACGACGAA TACGACAGCA ACGCTTGATC 
TCTATAGGCA AATGGATTGC TGATAATCAG CCAAGGCTGA TTCAGTGTGA AAATGAGGTA 
GGGAAATTGT TGTTTATCAC AGAAATCCCA GAATTAATAC TGGAAGACCC CAGTGAAGCC 
AAAGAGAACC TCATTCTGCA AGAAACATCT GTGATAGAGT CGCTGGCTGC AGATGGGAGC 
CCAGGGCTAA AATCAGTGCT ATCTACAAGC CGAAATTTAA GCAACAACTG TGACACAGGA 
GAGAAGCCAG TGGTTACCTT CAAAGAAAAC ATTAAGACAC GAGAAGTGAA CAGAGACCAA 
GGAAGAAGTT TTCCTCCCAA AGAGGTAAAA TCCCAGACAG AACTAAGAAA GACTCCAGTG 
TCTGAAGCCA GAAAAACACC TGTAACTCAA ACCCCAACTC AAGCAAGTAA CTCCCAGTTC 
ATCCCCATTC ATCACCCTGG AGCCTTCCCT CCTCTTCCCA GCAGGCCAGG GTTTCCGCCC 
CCAACATATG TTATCCCCCC GCCTGTGGCA TTTTCTATGG GCTCAGGTTA CACCTTCCCA 
GCTGGTGTTT CTGTCCCAGG AACCTTTCTT CAGCCTACAG CTCACTCTCC AGCAGGAAAC 
CAGGTGCAAG CTGGGAAACA GTCCCACATT CCTTACAGCC AGCAACGGCC CTCTGGACCA 
GGGCCAATGA ACCAGGGACC TCAACAATCA CAGCCACCTT CCCAGCAACC CCTTACATCT 
TTACCAGCTC AGCCAACAGC ACAGTCTACA AGCCAGCTGC AGGTTCAAGC TCTAACTCAG 
CAACAACAAT CCCCTACAAA AGCTGTGCCG GCTTTGGGGA AAAGCCCGCC TCACCACTCT 
GGATTCCAGC AGTATCAACA GGCAGATGCC TCCAAACAGC TGTGGAATCC CCCTCAGGTT 
CAAGGCCCAT TAGGGAAAAT TATGCCTGTG AAACAGCCCT ACTACCTTCA GACCCAAGAC 
CCCATAAAAC TGTTTGAGCC GTCATTGCAA CCTCCTGTAA TGCAGCAGCA GCCTCTAGAA 
AAAAAAATGA AGCCTTTTCC CATGGAGCCA TATAACCATA ATCCCTCAGA AGTCAAGGTC 
CCAGAATTCT ACTGGGATTC TTCCTACAGC ATGGCTGATA ACAGATCTGT AATGGCACAG 
CAAGCAAACA TAGACCGCAG GGGCAAACGG TCACCAGGAG TCTTCCGTCC AGAGCAGGAT 
CCTGTACCCA GAATGCCGTT TGAGGACCCC AAGAGCTCCC CTCTGCTTCC TCCGGACCTG 
TTAAAGAGTC TGGCTGCCTT GGAGGAAGAG GAAGAGCTGA TTTTTTCTAA CCCTCCTGAT 
CTTTACCCGG CTCTGCTGGG GCCTCTCGCC TCTCTTCCTG GACGAAGCCT TTTTAAATCC 
TTATTGGAGA AGCCCTCAGA GCTCATGTCA CATTCATCCT CTTTCCTGTC CCTCACCGGA 
TTCTCTCTCA ATCAGGAAAG ATACCCAAAT AATAGTATGT TCAATGAGGT ATATGGGAAA 
AACCTGACAT CCAGCTCCAA AGCAGAACTC AGTCCCTCAA TGGCCCCCCA GGAAACATCT 
CTGTATTCCC TTTTTGAAGG GACTCCGTGG TCTCCATCAC TTCCTGCCAG TTCAGATCAT 
TCAACACCAG CCAGCCAGTC TCCTCATTCC TCTAACCCAA GCAGCCTACC CAGCTCTCCT 
CCAACACACA ACCATAATTC TGTTCCATTC TCCAATTTTG GACCCATTGG GACTCCAGAT 
AACAGGGATA GAAGGACTGC AGATCGGTGG AAAACTGATA AGCCAGCCAT GGGTGGGTTT 
GGCATTGATT ATCTCTCAGC AACGTCATCC TCTGAGAGCA GTTGGCATCA GGCCAGCACT 
CCGAGTGGCA CCTGGACAGG CCATGGCCCT TCCATGGAGG ATTCCTCTGC TGTCCTCATG 
GAAAGCCTAA AGAAGCAACA GCATGGGGTC CAGCAGTTGG GGCCCAAAAG ACAGTCTGAA 
GAGGAAGGAA GCAGCAGTAT CTGCGTAGCC CACAGAGGGC CCAGGCCCCT GCCCAGCTGC 
AGTCTCCCAG CCTCCACTTT CAGAGTGAAA TTCAAGGCAG CACGGACATG TGCCCATCAG 
GCACAGAAGA AAACACGACG TCGTCCATTT TGGAAGAGAC GAAAGAAAGG AAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_963863.2
CDS	123..3659
Translation	MSLQSAQYLRQAEVLKADMTDSKLGPAEVWTSRQALQDLYQKMLVTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRANPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQTHTSAIVKPQSSSCSYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNLQKALSKALESRDEVKTKWGVSDFIKAFIKFHGHVYLSKSLEKLSPLREKLEEQFKRLLFQKAFNSQQLVHVTVINLFQLHHLRDFSNETEQHTYSQDEQLCWTQLLALFMSFLGILCKCPLQNESQEESYNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPHEEDLSSISATPLPEEFELQGFLALRPSFRNLDFSKGHQGITGDKEGQQRRIRQQRLISIGKWIADNQPRLIQCENEVGKLLFITEIPELILEDPSEAKENLILQETSVIESLAADGSPGLKSVLSTSRNLSNNCDTGEKPVVTFKENIKTREVNRDQGRSFPPKEVKSQTELRKTPVSEARKTPVTQTPTQASNSQFIPIHHPGAFPPLPSRPGFPPPTYVIPPPVAFSMGSGYTFPAGVSVPGTFLQPTAHSPAGNQVQAGKQSHIPYSQQRPSGPGPMNQGPQQSQPPSQQPLTSLPAQPTAQSTSQLQVQALTQQQQSPTKAVPALGKSPPHHSGFQQYQQADASKQLWNPPQVQGPLGKIMPVKQPYYLQTQDPIKLFEPSLQPPVMQQQPLEKKMKPFPMEPYNHNPSEVKVPEFYWDSSYSMADNRSVMAQQANIDRRGKRSPGVFRPEQDPVPRMPFEDPKSSPLLPPDLLKSLAALEEEEELIFSNPPDLYPALLGPLASLPGRSLFKSLLEKPSELMSHSSSFLSLTGFSLNQERYPNNSMFNEVYGKNLTSSSKAELSPSMAPQETSLYSLFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSVPFSNFGPIGTPDNRDRRTADRWKTDKPAMGGFGIDYLSATSSSESSWHQASTPSGTWTGHGPSMEDSSAVLMESLKKQQHGVQQLGPKRQSEEEGSSSICVAHRGPRPLPSCSLPASTFRVKFKAARTCAHQAQKKTRRRPFWKRRKKGK

Target ORF information:

RefSeq Version	NM_201569.3
Organism	Homo sapiens(human)
Definition	Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_201569.3

ORF Insert Sequence:

ATGAGCCTGC AGAGCGCGCA GTACCTCCGG CAGGCAGAAG TCCTGAAGGC TGACATGACA 
GATTCTAAGC TGGGTCCAGC TGAAGTCTGG ACATCCAGGC AGGCTCTGCA GGACCTGTAC 
CAGAAAATGC TAGTTACCGA TTTGGAATAC GCTTTAGACA AGAAAGTAGA ACAGGATCTC 
TGGAATCACG CCTTTAAGAA TCAGATCACA ACACTGCAAG GCCAGGCAAA GAATCGAGCA 
AATCCGAATC GGAGTGAAGT TCAGGCAAAC CTTTCTCTGT TCCTAGAGGC AGCTAGTGGC 
TTCTATACTC AGTTATTACA AGAACTGTGT ACAGTATTTA ATGTAGATTT ACCATGCCGT 
GTGAAGTCTT CCCAATTGGG AATTATCAGC AATAAACAGA CGCATACCAG CGCCATAGTG 
AAGCCACAGT CTAGCTCCTG TTCCTATATC TGCCAGCACT GCCTCGTCCA CCTTGGAGAC 
ATTGCTCGAT ACAGAAACCA GACCAGCCAG GCAGAGTCCT ACTATAGGCA TGCAGCTCAG 
CTTGTCCCCT CCAATGGTCA GCCTTATAAT CAGTTGGCTA TCTTAGCTTC TTCCAAAGGA 
GACCATCTGA CCACAATTTT CTACTACTGC AGAAGCATTG CTGTGAAGTT CCCTTTCCCA 
GCTGCCTCCA CTAATCTGCA AAAAGCACTT TCTAAAGCAC TGGAAAGCCG AGATGAGGTG 
AAAACCAAGT GGGGTGTTTC TGACTTCATC AAGGCCTTTA TTAAATTCCA CGGTCATGTG 
TACCTGAGTA AGAGCTTGGA AAAGTTGAGC CCTCTTCGAG AGAAATTGGA AGAACAGTTT 
AAGAGGCTGC TATTCCAAAA AGCTTTCAAC TCTCAGCAGT TAGTTCATGT CACTGTCATT 
AACCTGTTTC AACTTCATCA CCTTCGTGAC TTTAGCAATG AAACCGAGCA GCACACTTAT 
AGCCAAGATG AGCAGCTATG TTGGACACAG TTGCTGGCCC TCTTTATGTC TTTTCTTGGC 
ATCCTGTGCA AGTGTCCTCT ACAGAATGAG TCTCAGGAGG AGTCCTACAA TGCCTATCCT 
CTTCCAGCAG TCAAGGTCTC CATGGACTGG CTAAGACTCA GACCCAGGGT CTTTCAGGAG 
GCAGTGGTGG ATGAAAGACA GTACATTTGG CCCTGGTTGA TTTCTCTTCT GAATAGTTTC 
CATCCCCATG AAGAGGACCT CTCAAGTATT AGTGCGACAC CACTTCCAGA GGAGTTTGAA 
TTACAAGGAT TTTTGGCATT GAGACCTTCT TTCAGGAACT TGGATTTTTC CAAAGGTCAC 
CAGGGTATTA CAGGGGACAA AGAAGGCCAG CAACGACGAA TACGACAGCA ACGCTTGATC 
TCTATAGGCA AATGGATTGC TGATAATCAG CCAAGGCTGA TTCAGTGTGA AAATGAGGTA 
GGGAAATTGT TGTTTATCAC AGAAATCCCA GAATTAATAC TGGAAGACCC CAGTGAAGCC 
AAAGAGAACC TCATTCTGCA AGAAACATCT GTGATAGAGT CGCTGGCTGC AGATGGGAGC 
CCAGGGCTAA AATCAGTGCT ATCTACAAGC CGAAATTTAA GCAACAACTG TGACACAGGA 
GAGAAGCCAG TGGTTACCTT CAAAGAAAAC ATTAAGACAC GAGAAGTGAA CAGAGACCAA 
GGAAGAAGTT TTCCTCCCAA AGAGGTAAAA TCCCAGACAG AACTAAGAAA GACTCCAGTG 
TCTGAAGCCA GAAAAACACC TGTAACTCAA ACCCCAACTC AAGCAAGTAA CTCCCAGTTC 
ATCCCCATTC ATCACCCTGG AGCCTTCCCT CCTCTTCCCA GCAGGCCAGG GTTTCCGCCC 
CCAACATATG TTATCCCCCC GCCTGTGGCA TTTTCTATGG GCTCAGGTTA CACCTTCCCA 
GCTGGTGTTT CTGTCCCAGG AACCTTTCTT CAGCCTACAG CTCACTCTCC AGCAGGAAAC 
CAGGTGCAAG CTGGGAAACA GTCCCACATT CCTTACAGCC AGCAACGGCC CTCTGGACCA 
GGGCCAATGA ACCAGGGACC TCAACAATCA CAGCCACCTT CCCAGCAACC CCTTACATCT 
TTACCAGCTC AGCCAACAGC ACAGTCTACA AGCCAGCTGC AGGTTCAAGC TCTAACTCAG 
CAACAACAAT CCCCTACAAA AGCTGTGCCG GCTTTGGGGA AAAGCCCGCC TCACCACTCT 
GGATTCCAGC AGTATCAACA GGCAGATGCC TCCAAACAGC TGTGGAATCC CCCTCAGGTT 
CAAGGCCCAT TAGGGAAAAT TATGCCTGTG AAACAGCCCT ACTACCTTCA GACCCAAGAC 
CCCATAAAAC TGTTTGAGCC GTCATTGCAA CCTCCTGTAA TGCAGCAGCA GCCTCTAGAA 
AAAAAAATGA AGCCTTTTCC CATGGAGCCA TATAACCATA ATCCCTCAGA AGTCAAGGTC 
CCAGAATTCT ACTGGGATTC TTCCTACAGC ATGGCTGATA ACAGATCTGT AATGGCACAG 
CAAGCAAACA TAGACCGCAG GGGCAAACGG TCACCAGGAG TCTTCCGTCC AGAGCAGGAT 
CCTGTACCCA GAATGCCGTT TGAGGACCCC AAGAGCTCCC CTCTGCTTCC TCCGGACCTG 
TTAAAGAGTC TGGCTGCCTT GGAGGAAGAG GAAGAGCTGA TTTTTTCTAA CCCTCCTGAT 
CTTTACCCGG CTCTGCTGGG GCCTCTCGCC TCTCTTCCTG GACGAAGCCT TTTTAAATCC 
TTATTGGAGA AGCCCTCAGA GCTCATGTCA CATTCATCCT CTTTCCTGTC CCTCACCGGA 
TTCTCTCTCA ATCAGGAAAG ATACCCAAAT AATAGTATGT TCAATGAGGT ATATGGGAAA 
AACCTGACAT CCAGCTCCAA AGCAGAACTC AGTCCCTCAA TGGCCCCCCA GGAAACATCT 
CTGTATTCCC TTTTTGAAGG GACTCCGTGG TCTCCATCAC TTCCTGCCAG TTCAGATCAT 
TCAACACCAG CCAGCCAGTC TCCTCATTCC TCTAACCCAA GCAGCCTACC CAGCTCTCCT 
CCAACACACA ACCATAATTC TGTTCCATTC TCCAATTTTG GACCCATTGG GACTCCAGAT 
AACAGGGATA GAAGGACTGC AGATCGGTGG AAAACTGATA AGCCAGCCAT GGGTGGGTTT 
GGCATTGATT ATCTCTCAGC AACGTCATCC TCTGAGAGCA GTTGGCATCA GGCCAGCACT 
CCGAGTGGCA CCTGGACAGG CCATGGCCCT TCCATGGAGG ATTCCTCTGC TGTCCTCATG 
GAAAGCCTAA AGAAGCAACA GCATGGGGTC CAGCAGTTGG GGCCCAAAAG ACAGTCTGAA 
GAGGAAGGAA GCAGCAGTAT CTGCGTAGCC CACAGAGGGC CCAGGCCCCT GCCCAGCTGC 
AGTCTCCCAG CCTCCACTTT CAGAGTGAAA TTCAAGGCAG CACGGACATG TGCCCATCAG 
GCACAGAAGA AAACACGACG TCGTCCATTT TGGAAGAGAC GAAAGAAAGG AAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG7 ( NM_001350220.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001337149.1 Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 8, mRNA.

Price & Availability↑

CloneID	OHu34879
Clone ID Related Accession (Same CDS sequence)	NM_001350220.1 , XM_011510205.2 , NM_001350220.2 , XM_005245649.3 , XM_011510205.3 , XM_005245649.4
Accession Version	NM_001350220.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3651bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-27
Organism	Homo sapiens(human)
Product	protein SMG7 isoform 8
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB223038.1, AB085674.1, AL137800.12, DC341780.1, D87437.2, CR995963.1, DA238539.1, DA057117.1 and CN479993.1. On Apr 10, 2017 this sequence version replaced XM_005245648.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.223290.1, SRR1803612.198442.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
CAGATGAGGA CCGAAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG 
GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCTGA AGTCTGGACA 
TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTAG TTACCGATTT GGAATACGCT 
TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCACGCCT TTAAGAATCA GATCACAACA 
CTGCAAGGCC AGGCAAAGAA TCGAGCAAAT CCGAATCGGA GTGAAGTTCA GGCAAACCTT 
TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA 
GTATTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AATTGGGAAT TATCAGCAAT 
AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC 
CAGCACTGCC TCGTCCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA 
GAGTCCTACT ATAGGCATGC AGCTCAGCTT GTCCCCTCCA ATGGTCAGCC TTATAATCAG 
TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACCA CAATTTTCTA CTACTGCAGA 
AGCATTGCTG TGAAGTTCCC TTTCCCAGCT GCCTCCACTA ATCTGCAAAA AGCACTTTCT 
AAAGCACTGG AAAGCCGAGA TGAGGTGAAA ACCAAGTGGG GTGTTTCTGA CTTCATCAAG 
GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT 
CTTCGAGAGA AATTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAAAAAGC TTTCAACTCT 
CAGCAGTTAG TTCATGTCAC TGTCATTAAC CTGTTTCAAC TTCATCACCT TCGTGACTTT 
AGCAATGAAA CCGAGCAGCA CACTTATAGC CAAGATGAGC AGCTATGTTG GACACAGTTG 
CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTACA GAATGAGTCT 
CAGGAGGAGT CCTACAATGC CTATCCTCTT CCAGCAGTCA AGGTCTCCAT GGACTGGCTA 
AGACTCAGAC CCAGGGTCTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCC 
TGGTTGATTT CTCTTCTGAA TAGTTTCCAT CCCCATGAAG AGGACCTCTC AAGTATTAGT 
GCGACACCAC TTCCAGAGGA GTTTGAATTA CAAGGATTTT TGGCATTGAG ACCTTCTTTC 
AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GGGACAAAGA AGGCCAGCAA 
CGACGAATAC GACAGCAACG CTTGATCTCT ATAGGCAAAT GGATTGCTGA TAATCAGCCA 
AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAA 
TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA AACATCTGTG 
ATAGAGTCGC TGGCTGCAGA TGGGAGCCCA GGGCTAAAAT CAGTGCTATC TACAAGCCGA 
AATTTAAGCA ACAACTGTGA CACAGGAGAG AAGCCAGTGG TTACCTTCAA AGAAAACATT 
AAGACACGAG AAGTGAACAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTGAGAAGG 
GACTATAGCA AAGGAATAAC TGTAACTAAG AATGATGGAA AGAAGGACAA CAACAAGAGG 
AAAACTGAAA CCAAGAAATG CACCTTAGAA AAGTTACAGG AAACAGGAAA GCAGAATGTG 
GCAGTGCAGG TAAAATCCCA GACAGAACTA AGAAAGACTC CAGTGTCTGA AGCCAGAAAA 
ACACCTGTAA CTCAAACCCC AACTCAAGCA AGTAACTCCC AGTTCATCCC CATTCATCAC 
CCTGGAGCCT TCCCTCCTCT TCCCAGCAGG CCAGGGTTTC CGCCCCCAAC ATATGTTATC 
CCCCCGCCTG TGGCATTTTC TATGGGCTCA GGTTACACCT TCCCAGCTGG TGTTTCTGTC 
CCAGGAACCT TTCTTCAGCC TACAGCTCAC TCTCCAGCAG GAAACCAGGT GCAAGCTGGG 
AAACAGTCCC ACATTCCTTA CAGCCAGCAA CGGCCCTCTG GACCAGGGCC AATGAACCAG 
GGACCTCAAC AATCACAGCC ACCTTCCCAG CAACCCCTTA CATCTTTACC AGCTCAGCCA 
ACAGCACAGT CTACAAGCCA GCTGCAGGTT CAAGCTCTAA CTCAGCAACA ACAATCCCCT 
ACAAAAGCTG TGCCGGCTTT GGGGAAAAGC CCGCCTCACC ACTCTGGATT CCAGCAGTAT 
CAACAGGCAG ATGCCTCCAA ACAGCTGTGG AATCCCCCTC AGGTTCAAGG CCCATTAGGG 
AAAATTATGC CTGTGAAACA GCCCTACTAC CTTCAGACCC AAGACCCCAT AAAACTGTTT 
GAGCCGTCAT TGCAACCTCC TGTAATGCAG CAGCAGCCTC TAGAAAAAAA AATGAAGCCT 
TTTCCCATGG AGCCATATAA CCATAATCCC TCAGAAGTCA AGGTCCCAGA ATTCTACTGG 
GATTCTTCCT ACAGCATGGC TGATAACAGA TCTGTAATGG CACAGCAAGC AAACATAGAC 
CGCAGGGGCA AACGGTCACC AGGAGTCTTC CGTCCAGAGC AGGATCCTGT ACCCAGAATG 
CCGTTTGAGG ACCCCAAGAG CTCCCCTCTG CTTCCTCCGG ACCTGTTAAA GAGTCTGGCT 
GCCTTGGAGG AAGAGGAAGA GCTGATTTTT TCTAACCCTC CTGATCTTTA CCCGGCTCTG 
CTGGGGCCTC TCGCCTCTCT TCCTGGACGA AGCCTTTTTA AATCCTTATT GGAGAAGCCC 
TCAGAGCTCA TGTCACATTC ATCCTCTTTC CTGTCCCTCA CCGGATTCTC TCTCAATCAG 
GAAAGATACC CAAATAATAG TATGTTCAAT GAGGTATATG GGAAAAACCT GACATCCAGC 
TCCAAAGCAG AACTCAGTCC CTCAATGGCC CCCCAGGAAA CATCTCTGTA TTCCCTTTTT 
GAAGGGACTC CGTGGTCTCC ATCACTTCCT GCCAGTTCAG ATCATTCAAC ACCAGCCAGC 
CAGTCTCCTC ATTCCTCTAA CCCAAGCAGC CTACCCAGCT CTCCTCCAAC ACACAACCAT 
AATTCTGTTC CATTCTCCAA TTTTGGACCC ATTGGGACTC CAGATAACAG GGATAGAAGG 
ACTGCAGATC GGTGGAAAAC TGATAAGCCA GCCATGGGTG GGTTTGGCAT TGATTATCTC 
TCAGCAACGT CATCCTCTGA GAGCAGTTGG CATCAGGCCA GCACTCCGAG TGGCACCTGG 
ACAGGCCATG GCCCTTCCAT GGAGGATTCC TCTGCTGTCC TCATGGAAAG CCTAAAGTCT 
ATCTGGTCCA GTTCCATGAT GCATCCTGGA CCTTCTGCTC TGGAGCAGCT GTTAATGCAG 
CAGAAGCAGA AACAGCAACG GGGACAAGGC ACCATGAACC CTCCACACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001337149.1
CDS	440..4090
Translation	MRNWMFLQIVIVGENVSFKSQMRTENLKSEEHLKSSNIRQAEVLKADMTDSKLGPAEVWTSRQALQDLYQKMLVTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRANPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQTHTSAIVKPQSSSCSYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNLQKALSKALESRDEVKTKWGVSDFIKAFIKFHGHVYLSKSLEKLSPLREKLEEQFKRLLFQKAFNSQQLVHVTVINLFQLHHLRDFSNETEQHTYSQDEQLCWTQLLALFMSFLGILCKCPLQNESQEESYNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPHEEDLSSISATPLPEEFELQGFLALRPSFRNLDFSKGHQGITGDKEGQQRRIRQQRLISIGKWIADNQPRLIQCENEVGKLLFITEIPELILEDPSEAKENLILQETSVIESLAADGSPGLKSVLSTSRNLSNNCDTGEKPVVTFKENIKTREVNRDQGRSFPPKEVRRDYSKGITVTKNDGKKDNNKRKTETKKCTLEKLQETGKQNVAVQVKSQTELRKTPVSEARKTPVTQTPTQASNSQFIPIHHPGAFPPLPSRPGFPPPTYVIPPPVAFSMGSGYTFPAGVSVPGTFLQPTAHSPAGNQVQAGKQSHIPYSQQRPSGPGPMNQGPQQSQPPSQQPLTSLPAQPTAQSTSQLQVQALTQQQQSPTKAVPALGKSPPHHSGFQQYQQADASKQLWNPPQVQGPLGKIMPVKQPYYLQTQDPIKLFEPSLQPPVMQQQPLEKKMKPFPMEPYNHNPSEVKVPEFYWDSSYSMADNRSVMAQQANIDRRGKRSPGVFRPEQDPVPRMPFEDPKSSPLLPPDLLKSLAALEEEEELIFSNPPDLYPALLGPLASLPGRSLFKSLLEKPSELMSHSSSFLSLTGFSLNQERYPNNSMFNEVYGKNLTSSSKAELSPSMAPQETSLYSLFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSVPFSNFGPIGTPDNRDRRTADRWKTDKPAMGGFGIDYLSATSSSESSWHQASTPSGTWTGHGPSMEDSSAVLMESLKSIWSSSMMHPGPSALEQLLMQQKQKQQRGQGTMNPPH

Target ORF information:

RefSeq Version	NM_001350220.1
Organism	Homo sapiens(human)
Definition	Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001350220.1

ORF Insert Sequence:

ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
CAGATGAGGA CCGAAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG 
GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCTGA AGTCTGGACA 
TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTAG TTACCGATTT GGAATACGCT 
TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCACGCCT TTAAGAATCA GATCACAACA 
CTGCAAGGCC AGGCAAAGAA TCGAGCAAAT CCGAATCGGA GTGAAGTTCA GGCAAACCTT 
TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA 
GTATTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AATTGGGAAT TATCAGCAAT 
AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC 
CAGCACTGCC TCGTCCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA 
GAGTCCTACT ATAGGCATGC AGCTCAGCTT GTCCCCTCCA ATGGTCAGCC TTATAATCAG 
TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACCA CAATTTTCTA CTACTGCAGA 
AGCATTGCTG TGAAGTTCCC TTTCCCAGCT GCCTCCACTA ATCTGCAAAA AGCACTTTCT 
AAAGCACTGG AAAGCCGAGA TGAGGTGAAA ACCAAGTGGG GTGTTTCTGA CTTCATCAAG 
GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT 
CTTCGAGAGA AATTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAAAAAGC TTTCAACTCT 
CAGCAGTTAG TTCATGTCAC TGTCATTAAC CTGTTTCAAC TTCATCACCT TCGTGACTTT 
AGCAATGAAA CCGAGCAGCA CACTTATAGC CAAGATGAGC AGCTATGTTG GACACAGTTG 
CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTACA GAATGAGTCT 
CAGGAGGAGT CCTACAATGC CTATCCTCTT CCAGCAGTCA AGGTCTCCAT GGACTGGCTA 
AGACTCAGAC CCAGGGTCTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCC 
TGGTTGATTT CTCTTCTGAA TAGTTTCCAT CCCCATGAAG AGGACCTCTC AAGTATTAGT 
GCGACACCAC TTCCAGAGGA GTTTGAATTA CAAGGATTTT TGGCATTGAG ACCTTCTTTC 
AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GGGACAAAGA AGGCCAGCAA 
CGACGAATAC GACAGCAACG CTTGATCTCT ATAGGCAAAT GGATTGCTGA TAATCAGCCA 
AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAA 
TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA AACATCTGTG 
ATAGAGTCGC TGGCTGCAGA TGGGAGCCCA GGGCTAAAAT CAGTGCTATC TACAAGCCGA 
AATTTAAGCA ACAACTGTGA CACAGGAGAG AAGCCAGTGG TTACCTTCAA AGAAAACATT 
AAGACACGAG AAGTGAACAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTGAGAAGG 
GACTATAGCA AAGGAATAAC TGTAACTAAG AATGATGGAA AGAAGGACAA CAACAAGAGG 
AAAACTGAAA CCAAGAAATG CACCTTAGAA AAGTTACAGG AAACAGGAAA GCAGAATGTG 
GCAGTGCAGG TAAAATCCCA GACAGAACTA AGAAAGACTC CAGTGTCTGA AGCCAGAAAA 
ACACCTGTAA CTCAAACCCC AACTCAAGCA AGTAACTCCC AGTTCATCCC CATTCATCAC 
CCTGGAGCCT TCCCTCCTCT TCCCAGCAGG CCAGGGTTTC CGCCCCCAAC ATATGTTATC 
CCCCCGCCTG TGGCATTTTC TATGGGCTCA GGTTACACCT TCCCAGCTGG TGTTTCTGTC 
CCAGGAACCT TTCTTCAGCC TACAGCTCAC TCTCCAGCAG GAAACCAGGT GCAAGCTGGG 
AAACAGTCCC ACATTCCTTA CAGCCAGCAA CGGCCCTCTG GACCAGGGCC AATGAACCAG 
GGACCTCAAC AATCACAGCC ACCTTCCCAG CAACCCCTTA CATCTTTACC AGCTCAGCCA 
ACAGCACAGT CTACAAGCCA GCTGCAGGTT CAAGCTCTAA CTCAGCAACA ACAATCCCCT 
ACAAAAGCTG TGCCGGCTTT GGGGAAAAGC CCGCCTCACC ACTCTGGATT CCAGCAGTAT 
CAACAGGCAG ATGCCTCCAA ACAGCTGTGG AATCCCCCTC AGGTTCAAGG CCCATTAGGG 
AAAATTATGC CTGTGAAACA GCCCTACTAC CTTCAGACCC AAGACCCCAT AAAACTGTTT 
GAGCCGTCAT TGCAACCTCC TGTAATGCAG CAGCAGCCTC TAGAAAAAAA AATGAAGCCT 
TTTCCCATGG AGCCATATAA CCATAATCCC TCAGAAGTCA AGGTCCCAGA ATTCTACTGG 
GATTCTTCCT ACAGCATGGC TGATAACAGA TCTGTAATGG CACAGCAAGC AAACATAGAC 
CGCAGGGGCA AACGGTCACC AGGAGTCTTC CGTCCAGAGC AGGATCCTGT ACCCAGAATG 
CCGTTTGAGG ACCCCAAGAG CTCCCCTCTG CTTCCTCCGG ACCTGTTAAA GAGTCTGGCT 
GCCTTGGAGG AAGAGGAAGA GCTGATTTTT TCTAACCCTC CTGATCTTTA CCCGGCTCTG 
CTGGGGCCTC TCGCCTCTCT TCCTGGACGA AGCCTTTTTA AATCCTTATT GGAGAAGCCC 
TCAGAGCTCA TGTCACATTC ATCCTCTTTC CTGTCCCTCA CCGGATTCTC TCTCAATCAG 
GAAAGATACC CAAATAATAG TATGTTCAAT GAGGTATATG GGAAAAACCT GACATCCAGC 
TCCAAAGCAG AACTCAGTCC CTCAATGGCC CCCCAGGAAA CATCTCTGTA TTCCCTTTTT 
GAAGGGACTC CGTGGTCTCC ATCACTTCCT GCCAGTTCAG ATCATTCAAC ACCAGCCAGC 
CAGTCTCCTC ATTCCTCTAA CCCAAGCAGC CTACCCAGCT CTCCTCCAAC ACACAACCAT 
AATTCTGTTC CATTCTCCAA TTTTGGACCC ATTGGGACTC CAGATAACAG GGATAGAAGG 
ACTGCAGATC GGTGGAAAAC TGATAAGCCA GCCATGGGTG GGTTTGGCAT TGATTATCTC 
TCAGCAACGT CATCCTCTGA GAGCAGTTGG CATCAGGCCA GCACTCCGAG TGGCACCTGG 
ACAGGCCATG GCCCTTCCAT GGAGGATTCC TCTGCTGTCC TCATGGAAAG CCTAAAGTCT 
ATCTGGTCCA GTTCCATGAT GCATCCTGGA CCTTCTGCTC TGGAGCAGCT GTTAATGCAG 
CAGAAGCAGA AACAGCAACG GGGACAAGGC ACCATGAACC CTCCACACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG7 ( NM_001350220.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001337149.1 Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 8, mRNA.

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CloneID	OHu34879
Clone ID Related Accession (Same CDS sequence)	NM_001350220.1 , XM_011510205.2 , NM_001350220.2 , XM_005245649.3 , XM_011510205.3 , XM_005245649.4
Accession Version	NM_001350220.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3651bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-28
Organism	Homo sapiens(human)
Product	protein SMG7 isoform 8
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB223038.1, AB085674.1, AL137800.12, DC341780.1, D87437.2, CR995963.1, DA238539.1, DA057117.1 and CN479993.1. On Jun 2, 2019 this sequence version replaced NM_001350220.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.223290.1, SRR1803612.198442.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
CAGATGAGGA CCGAAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG 
GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCTGA AGTCTGGACA 
TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTAG TTACCGATTT GGAATACGCT 
TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCACGCCT TTAAGAATCA GATCACAACA 
CTGCAAGGCC AGGCAAAGAA TCGAGCAAAT CCGAATCGGA GTGAAGTTCA GGCAAACCTT 
TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA 
GTATTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AATTGGGAAT TATCAGCAAT 
AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC 
CAGCACTGCC TCGTCCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA 
GAGTCCTACT ATAGGCATGC AGCTCAGCTT GTCCCCTCCA ATGGTCAGCC TTATAATCAG 
TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACCA CAATTTTCTA CTACTGCAGA 
AGCATTGCTG TGAAGTTCCC TTTCCCAGCT GCCTCCACTA ATCTGCAAAA AGCACTTTCT 
AAAGCACTGG AAAGCCGAGA TGAGGTGAAA ACCAAGTGGG GTGTTTCTGA CTTCATCAAG 
GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT 
CTTCGAGAGA AATTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAAAAAGC TTTCAACTCT 
CAGCAGTTAG TTCATGTCAC TGTCATTAAC CTGTTTCAAC TTCATCACCT TCGTGACTTT 
AGCAATGAAA CCGAGCAGCA CACTTATAGC CAAGATGAGC AGCTATGTTG GACACAGTTG 
CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTACA GAATGAGTCT 
CAGGAGGAGT CCTACAATGC CTATCCTCTT CCAGCAGTCA AGGTCTCCAT GGACTGGCTA 
AGACTCAGAC CCAGGGTCTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCC 
TGGTTGATTT CTCTTCTGAA TAGTTTCCAT CCCCATGAAG AGGACCTCTC AAGTATTAGT 
GCGACACCAC TTCCAGAGGA GTTTGAATTA CAAGGATTTT TGGCATTGAG ACCTTCTTTC 
AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GGGACAAAGA AGGCCAGCAA 
CGACGAATAC GACAGCAACG CTTGATCTCT ATAGGCAAAT GGATTGCTGA TAATCAGCCA 
AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAA 
TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA AACATCTGTG 
ATAGAGTCGC TGGCTGCAGA TGGGAGCCCA GGGCTAAAAT CAGTGCTATC TACAAGCCGA 
AATTTAAGCA ACAACTGTGA CACAGGAGAG AAGCCAGTGG TTACCTTCAA AGAAAACATT 
AAGACACGAG AAGTGAACAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTGAGAAGG 
GACTATAGCA AAGGAATAAC TGTAACTAAG AATGATGGAA AGAAGGACAA CAACAAGAGG 
AAAACTGAAA CCAAGAAATG CACCTTAGAA AAGTTACAGG AAACAGGAAA GCAGAATGTG 
GCAGTGCAGG TAAAATCCCA GACAGAACTA AGAAAGACTC CAGTGTCTGA AGCCAGAAAA 
ACACCTGTAA CTCAAACCCC AACTCAAGCA AGTAACTCCC AGTTCATCCC CATTCATCAC 
CCTGGAGCCT TCCCTCCTCT TCCCAGCAGG CCAGGGTTTC CGCCCCCAAC ATATGTTATC 
CCCCCGCCTG TGGCATTTTC TATGGGCTCA GGTTACACCT TCCCAGCTGG TGTTTCTGTC 
CCAGGAACCT TTCTTCAGCC TACAGCTCAC TCTCCAGCAG GAAACCAGGT GCAAGCTGGG 
AAACAGTCCC ACATTCCTTA CAGCCAGCAA CGGCCCTCTG GACCAGGGCC AATGAACCAG 
GGACCTCAAC AATCACAGCC ACCTTCCCAG CAACCCCTTA CATCTTTACC AGCTCAGCCA 
ACAGCACAGT CTACAAGCCA GCTGCAGGTT CAAGCTCTAA CTCAGCAACA ACAATCCCCT 
ACAAAAGCTG TGCCGGCTTT GGGGAAAAGC CCGCCTCACC ACTCTGGATT CCAGCAGTAT 
CAACAGGCAG ATGCCTCCAA ACAGCTGTGG AATCCCCCTC AGGTTCAAGG CCCATTAGGG 
AAAATTATGC CTGTGAAACA GCCCTACTAC CTTCAGACCC AAGACCCCAT AAAACTGTTT 
GAGCCGTCAT TGCAACCTCC TGTAATGCAG CAGCAGCCTC TAGAAAAAAA AATGAAGCCT 
TTTCCCATGG AGCCATATAA CCATAATCCC TCAGAAGTCA AGGTCCCAGA ATTCTACTGG 
GATTCTTCCT ACAGCATGGC TGATAACAGA TCTGTAATGG CACAGCAAGC AAACATAGAC 
CGCAGGGGCA AACGGTCACC AGGAGTCTTC CGTCCAGAGC AGGATCCTGT ACCCAGAATG 
CCGTTTGAGG ACCCCAAGAG CTCCCCTCTG CTTCCTCCGG ACCTGTTAAA GAGTCTGGCT 
GCCTTGGAGG AAGAGGAAGA GCTGATTTTT TCTAACCCTC CTGATCTTTA CCCGGCTCTG 
CTGGGGCCTC TCGCCTCTCT TCCTGGACGA AGCCTTTTTA AATCCTTATT GGAGAAGCCC 
TCAGAGCTCA TGTCACATTC ATCCTCTTTC CTGTCCCTCA CCGGATTCTC TCTCAATCAG 
GAAAGATACC CAAATAATAG TATGTTCAAT GAGGTATATG GGAAAAACCT GACATCCAGC 
TCCAAAGCAG AACTCAGTCC CTCAATGGCC CCCCAGGAAA CATCTCTGTA TTCCCTTTTT 
GAAGGGACTC CGTGGTCTCC ATCACTTCCT GCCAGTTCAG ATCATTCAAC ACCAGCCAGC 
CAGTCTCCTC ATTCCTCTAA CCCAAGCAGC CTACCCAGCT CTCCTCCAAC ACACAACCAT 
AATTCTGTTC CATTCTCCAA TTTTGGACCC ATTGGGACTC CAGATAACAG GGATAGAAGG 
ACTGCAGATC GGTGGAAAAC TGATAAGCCA GCCATGGGTG GGTTTGGCAT TGATTATCTC 
TCAGCAACGT CATCCTCTGA GAGCAGTTGG CATCAGGCCA GCACTCCGAG TGGCACCTGG 
ACAGGCCATG GCCCTTCCAT GGAGGATTCC TCTGCTGTCC TCATGGAAAG CCTAAAGTCT 
ATCTGGTCCA GTTCCATGAT GCATCCTGGA CCTTCTGCTC TGGAGCAGCT GTTAATGCAG 
CAGAAGCAGA AACAGCAACG GGGACAAGGC ACCATGAACC CTCCACACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001337149.1
CDS	312..3962
Translation	MRNWMFLQIVIVGENVSFKSQMRTENLKSEEHLKSSNIRQAEVLKADMTDSKLGPAEVWTSRQALQDLYQKMLVTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRANPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQTHTSAIVKPQSSSCSYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNLQKALSKALESRDEVKTKWGVSDFIKAFIKFHGHVYLSKSLEKLSPLREKLEEQFKRLLFQKAFNSQQLVHVTVINLFQLHHLRDFSNETEQHTYSQDEQLCWTQLLALFMSFLGILCKCPLQNESQEESYNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPHEEDLSSISATPLPEEFELQGFLALRPSFRNLDFSKGHQGITGDKEGQQRRIRQQRLISIGKWIADNQPRLIQCENEVGKLLFITEIPELILEDPSEAKENLILQETSVIESLAADGSPGLKSVLSTSRNLSNNCDTGEKPVVTFKENIKTREVNRDQGRSFPPKEVRRDYSKGITVTKNDGKKDNNKRKTETKKCTLEKLQETGKQNVAVQVKSQTELRKTPVSEARKTPVTQTPTQASNSQFIPIHHPGAFPPLPSRPGFPPPTYVIPPPVAFSMGSGYTFPAGVSVPGTFLQPTAHSPAGNQVQAGKQSHIPYSQQRPSGPGPMNQGPQQSQPPSQQPLTSLPAQPTAQSTSQLQVQALTQQQQSPTKAVPALGKSPPHHSGFQQYQQADASKQLWNPPQVQGPLGKIMPVKQPYYLQTQDPIKLFEPSLQPPVMQQQPLEKKMKPFPMEPYNHNPSEVKVPEFYWDSSYSMADNRSVMAQQANIDRRGKRSPGVFRPEQDPVPRMPFEDPKSSPLLPPDLLKSLAALEEEEELIFSNPPDLYPALLGPLASLPGRSLFKSLLEKPSELMSHSSSFLSLTGFSLNQERYPNNSMFNEVYGKNLTSSSKAELSPSMAPQETSLYSLFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSVPFSNFGPIGTPDNRDRRTADRWKTDKPAMGGFGIDYLSATSSSESSWHQASTPSGTWTGHGPSMEDSSAVLMESLKSIWSSSMMHPGPSALEQLLMQQKQKQQRGQGTMNPPH

Target ORF information:

RefSeq Version	NM_001350220.2
Organism	Homo sapiens(human)
Definition	Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001350220.2

ORF Insert Sequence:

ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
CAGATGAGGA CCGAAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG 
GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCTGA AGTCTGGACA 
TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTAG TTACCGATTT GGAATACGCT 
TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCACGCCT TTAAGAATCA GATCACAACA 
CTGCAAGGCC AGGCAAAGAA TCGAGCAAAT CCGAATCGGA GTGAAGTTCA GGCAAACCTT 
TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA 
GTATTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AATTGGGAAT TATCAGCAAT 
AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC 
CAGCACTGCC TCGTCCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA 
GAGTCCTACT ATAGGCATGC AGCTCAGCTT GTCCCCTCCA ATGGTCAGCC TTATAATCAG 
TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACCA CAATTTTCTA CTACTGCAGA 
AGCATTGCTG TGAAGTTCCC TTTCCCAGCT GCCTCCACTA ATCTGCAAAA AGCACTTTCT 
AAAGCACTGG AAAGCCGAGA TGAGGTGAAA ACCAAGTGGG GTGTTTCTGA CTTCATCAAG 
GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT 
CTTCGAGAGA AATTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAAAAAGC TTTCAACTCT 
CAGCAGTTAG TTCATGTCAC TGTCATTAAC CTGTTTCAAC TTCATCACCT TCGTGACTTT 
AGCAATGAAA CCGAGCAGCA CACTTATAGC CAAGATGAGC AGCTATGTTG GACACAGTTG 
CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTACA GAATGAGTCT 
CAGGAGGAGT CCTACAATGC CTATCCTCTT CCAGCAGTCA AGGTCTCCAT GGACTGGCTA 
AGACTCAGAC CCAGGGTCTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCC 
TGGTTGATTT CTCTTCTGAA TAGTTTCCAT CCCCATGAAG AGGACCTCTC AAGTATTAGT 
GCGACACCAC TTCCAGAGGA GTTTGAATTA CAAGGATTTT TGGCATTGAG ACCTTCTTTC 
AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GGGACAAAGA AGGCCAGCAA 
CGACGAATAC GACAGCAACG CTTGATCTCT ATAGGCAAAT GGATTGCTGA TAATCAGCCA 
AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAA 
TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA AACATCTGTG 
ATAGAGTCGC TGGCTGCAGA TGGGAGCCCA GGGCTAAAAT CAGTGCTATC TACAAGCCGA 
AATTTAAGCA ACAACTGTGA CACAGGAGAG AAGCCAGTGG TTACCTTCAA AGAAAACATT 
AAGACACGAG AAGTGAACAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTGAGAAGG 
GACTATAGCA AAGGAATAAC TGTAACTAAG AATGATGGAA AGAAGGACAA CAACAAGAGG 
AAAACTGAAA CCAAGAAATG CACCTTAGAA AAGTTACAGG AAACAGGAAA GCAGAATGTG 
GCAGTGCAGG TAAAATCCCA GACAGAACTA AGAAAGACTC CAGTGTCTGA AGCCAGAAAA 
ACACCTGTAA CTCAAACCCC AACTCAAGCA AGTAACTCCC AGTTCATCCC CATTCATCAC 
CCTGGAGCCT TCCCTCCTCT TCCCAGCAGG CCAGGGTTTC CGCCCCCAAC ATATGTTATC 
CCCCCGCCTG TGGCATTTTC TATGGGCTCA GGTTACACCT TCCCAGCTGG TGTTTCTGTC 
CCAGGAACCT TTCTTCAGCC TACAGCTCAC TCTCCAGCAG GAAACCAGGT GCAAGCTGGG 
AAACAGTCCC ACATTCCTTA CAGCCAGCAA CGGCCCTCTG GACCAGGGCC AATGAACCAG 
GGACCTCAAC AATCACAGCC ACCTTCCCAG CAACCCCTTA CATCTTTACC AGCTCAGCCA 
ACAGCACAGT CTACAAGCCA GCTGCAGGTT CAAGCTCTAA CTCAGCAACA ACAATCCCCT 
ACAAAAGCTG TGCCGGCTTT GGGGAAAAGC CCGCCTCACC ACTCTGGATT CCAGCAGTAT 
CAACAGGCAG ATGCCTCCAA ACAGCTGTGG AATCCCCCTC AGGTTCAAGG CCCATTAGGG 
AAAATTATGC CTGTGAAACA GCCCTACTAC CTTCAGACCC AAGACCCCAT AAAACTGTTT 
GAGCCGTCAT TGCAACCTCC TGTAATGCAG CAGCAGCCTC TAGAAAAAAA AATGAAGCCT 
TTTCCCATGG AGCCATATAA CCATAATCCC TCAGAAGTCA AGGTCCCAGA ATTCTACTGG 
GATTCTTCCT ACAGCATGGC TGATAACAGA TCTGTAATGG CACAGCAAGC AAACATAGAC 
CGCAGGGGCA AACGGTCACC AGGAGTCTTC CGTCCAGAGC AGGATCCTGT ACCCAGAATG 
CCGTTTGAGG ACCCCAAGAG CTCCCCTCTG CTTCCTCCGG ACCTGTTAAA GAGTCTGGCT 
GCCTTGGAGG AAGAGGAAGA GCTGATTTTT TCTAACCCTC CTGATCTTTA CCCGGCTCTG 
CTGGGGCCTC TCGCCTCTCT TCCTGGACGA AGCCTTTTTA AATCCTTATT GGAGAAGCCC 
TCAGAGCTCA TGTCACATTC ATCCTCTTTC CTGTCCCTCA CCGGATTCTC TCTCAATCAG 
GAAAGATACC CAAATAATAG TATGTTCAAT GAGGTATATG GGAAAAACCT GACATCCAGC 
TCCAAAGCAG AACTCAGTCC CTCAATGGCC CCCCAGGAAA CATCTCTGTA TTCCCTTTTT 
GAAGGGACTC CGTGGTCTCC ATCACTTCCT GCCAGTTCAG ATCATTCAAC ACCAGCCAGC 
CAGTCTCCTC ATTCCTCTAA CCCAAGCAGC CTACCCAGCT CTCCTCCAAC ACACAACCAT 
AATTCTGTTC CATTCTCCAA TTTTGGACCC ATTGGGACTC CAGATAACAG GGATAGAAGG 
ACTGCAGATC GGTGGAAAAC TGATAAGCCA GCCATGGGTG GGTTTGGCAT TGATTATCTC 
TCAGCAACGT CATCCTCTGA GAGCAGTTGG CATCAGGCCA GCACTCCGAG TGGCACCTGG 
ACAGGCCATG GCCCTTCCAT GGAGGATTCC TCTGCTGTCC TCATGGAAAG CCTAAAGTCT 
ATCTGGTCCA GTTCCATGAT GCATCCTGGA CCTTCTGCTC TGGAGCAGCT GTTAATGCAG 
CAGAAGCAGA AACAGCAACG GGGACAAGGC ACCATGAACC CTCCACACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG7 ( NM_001331007.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317936.1 Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 6, mRNA.

Price & Availability↑

CloneID	OHu34883
Clone ID Related Accession (Same CDS sequence)	XM_011510206.2 , NM_001331007.2 , NM_001350221.1 , NM_001331007.1 , NM_001350221.2 , XM_011510206.3
Accession Version	NM_001331007.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3513bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-29
Organism	Homo sapiens(human)
Product	protein SMG7 isoform 6
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL449223.7 and AL137800.12. On Jun 2, 2019 this sequence version replaced NM_001331007.1. Transcript Variant: This variant (6) differs in the 5' UTR and coding sequence, uses an alternate in-frame splice junction, and contains an alternate in-frame exon compared to variant 1. The resulting isoform (6) has a longer and distinct N-terminus, contains an alternate internal segment, and lacks another alternate internal segment compared to isoform 1. Variants 6 and 9 encode the same protein (isoform 6). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.202323.1, SRR1803611.163447.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END##

ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
CAGATGAGGA CCGAAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG 
GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCTGA AGTCTGGACA 
TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTAG TTACCGATTT GGAATACGCT 
TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCACGCCT TTAAGAATCA GATCACAACA 
CTGCAAGGCC AGGCAAAGAA TCGAGCAAAT CCGAATCGGA GTGAAGTTCA GGCAAACCTT 
TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA 
GTATTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AATTGGGAAT TATCAGCAAT 
AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC 
CAGCACTGCC TCGTCCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA 
GAGTCCTACT ATAGGCATGC AGCTCAGCTT GTCCCCTCCA ATGGTCAGCC TTATAATCAG 
TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACCA CAATTTTCTA CTACTGCAGA 
AGCATTGCTG TGAAGTTCCC TTTCCCAGCT GCCTCCACTA ATCTGCAAAA AGCACTTTCT 
AAAGCACTGG AAAGCCGAGA TGAGGTGAAA ACCAAGTGGG GTGTTTCTGA CTTCATCAAG 
GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT 
CTTCGAGAGA AATTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAAAAAGC TTTCAACTCT 
CAGCAGTTAG TTCATGTCAC TGTCATTAAC CTGTTTCAAC TTCATCACCT TCGTGACTTT 
AGCAATGAAA CCGAGCAGCA CACTTATAGC CAAGATGAGC AGCTATGTTG GACACAGTTG 
CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTACA GAATGAGTCT 
CAGGAGGAGT CCTACAATGC CTATCCTCTT CCAGCAGTCA AGGTCTCCAT GGACTGGCTA 
AGACTCAGAC CCAGGGTCTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCC 
TGGTTGATTT CTCTTCTGAA TAGTTTCCAT CCCCATGAAG AGGACCTCTC AAGTATTAGT 
GCGACACCAC TTCCAGAGGA GTTTGAATTA CAAGGATTTT TGGCATTGAG ACCTTCTTTC 
AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GGGACAAAGA AGGCCAGCAA 
CGACGAATAC GACAGCAACG CTTGATCTCT ATAGGCAAAT GGATTGCTGA TAATCAGCCA 
AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAA 
TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA AACATCTGTG 
ATAGAGTCGC TGGCTGCAGA TGGGAGCCCA GGGCTAAAAT CAGTGCTATC TACAAGCCGA 
AATTTAAGCA ACAACTGTGA CACAGGAGAG AAGCCAGTGG TTACCTTCAA AGAAAACATT 
AAGACACGAG AAGTGAACAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTAAAATCC 
CAGACAGAAC TAAGAAAGAC TCCAGTGTCT GAAGCCAGAA AAACACCTGT AACTCAAACC 
CCAACTCAAG CAAGTAACTC CCAGTTCATC CCCATTCATC ACCCTGGAGC CTTCCCTCCT 
CTTCCCAGCA GGCCAGGGTT TCCGCCCCCA ACATATGTTA TCCCCCCGCC TGTGGCATTT 
TCTATGGGCT CAGGTTACAC CTTCCCAGCT GGTGTTTCTG TCCCAGGAAC CTTTCTTCAG 
CCTACAGCTC ACTCTCCAGC AGGAAACCAG GTGCAAGCTG GGAAACAGTC CCACATTCCT 
TACAGCCAGC AACGGCCCTC TGGACCAGGG CCAATGAACC AGGGACCTCA ACAATCACAG 
CCACCTTCCC AGCAACCCCT TACATCTTTA CCAGCTCAGC CAACAGCACA GTCTACAAGC 
CAGCTGCAGG TTCAAGCTCT AACTCAGCAA CAACAATCCC CTACAAAAGC TGTGCCGGCT 
TTGGGGAAAA GCCCGCCTCA CCACTCTGGA TTCCAGCAGT ATCAACAGGC AGATGCCTCC 
AAACAGCTGT GGAATCCCCC TCAGGTTCAA GGCCCATTAG GGAAAATTAT GCCTGTGAAA 
CAGCCCTACT ACCTTCAGAC CCAAGACCCC ATAAAACTGT TTGAGCCGTC ATTGCAACCT 
CCTGTAATGC AGCAGCAGCC TCTAGAAAAA AAAATGAAGC CTTTTCCCAT GGAGCCATAT 
AACCATAATC CCTCAGAAGT CAAGGTCCCA GAATTCTACT GGGATTCTTC CTACAGCATG 
GCTGATAACA GATCTGTAAT GGCACAGCAA GCAAACATAG ACCGCAGGGG CAAACGGTCA 
CCAGGAGTCT TCCGTCCAGA GCAGGATCCT GTACCCAGAA TGCCGTTTGA GGACCCCAAG 
AGCTCCCCTC TGCTTCCTCC GGACCTGTTA AAGAGTCTGG CTGCCTTGGA GGAAGAGGAA 
GAGCTGATTT TTTCTAACCC TCCTGATCTT TACCCGGCTC TGCTGGGGCC TCTCGCCTCT 
CTTCCTGGAC GAAGCCTTTT TAAATCCTTA TTGGAGAAGC CCTCAGAGCT CATGTCACAT 
TCATCCTCTT TCCTGTCCCT CACCGGATTC TCTCTCAATC AGGAAAGATA CCCAAATAAT 
AGTATGTTCA ATGAGGTATA TGGGAAAAAC CTGACATCCA GCTCCAAAGC AGAACTCAGT 
CCCTCAATGG CCCCCCAGGA AACATCTCTG TATTCCCTTT TTGAAGGGAC TCCGTGGTCT 
CCATCACTTC CTGCCAGTTC AGATCATTCA ACACCAGCCA GCCAGTCTCC TCATTCCTCT 
AACCCAAGCA GCCTACCCAG CTCTCCTCCA ACACACAACC ATAATTCTGT TCCATTCTCC 
AATTTTGGAC CCATTGGGAC TCCAGATAAC AGGGATAGAA GGACTGCAGA TCGGTGGAAA 
ACTGATAAGC CAGCCATGGG TGGGTTTGGC ATTGATTATC TCTCAGCAAC GTCATCCTCT 
GAGAGCAGTT GGCATCAGGC CAGCACTCCG AGTGGCACCT GGACAGGCCA TGGCCCTTCC 
ATGGAGGATT CCTCTGCTGT CCTCATGGAA AGCCTAAAGT CTATCTGGTC CAGTTCCATG 
ATGCATCCTG GACCTTCTGC TCTGGAGCAG CTGTTAATGC AGCAGAAGCA GAAACAGCAA 
CGGGGACAAG GCACCATGAA CCCTCCACAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317936.1
CDS	175..3687
Translation	MRNWMFLQIVIVGENVSFKSQMRTENLKSEEHLKSSNIRQAEVLKADMTDSKLGPAEVWTSRQALQDLYQKMLVTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRANPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQTHTSAIVKPQSSSCSYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNLQKALSKALESRDEVKTKWGVSDFIKAFIKFHGHVYLSKSLEKLSPLREKLEEQFKRLLFQKAFNSQQLVHVTVINLFQLHHLRDFSNETEQHTYSQDEQLCWTQLLALFMSFLGILCKCPLQNESQEESYNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPHEEDLSSISATPLPEEFELQGFLALRPSFRNLDFSKGHQGITGDKEGQQRRIRQQRLISIGKWIADNQPRLIQCENEVGKLLFITEIPELILEDPSEAKENLILQETSVIESLAADGSPGLKSVLSTSRNLSNNCDTGEKPVVTFKENIKTREVNRDQGRSFPPKEVKSQTELRKTPVSEARKTPVTQTPTQASNSQFIPIHHPGAFPPLPSRPGFPPPTYVIPPPVAFSMGSGYTFPAGVSVPGTFLQPTAHSPAGNQVQAGKQSHIPYSQQRPSGPGPMNQGPQQSQPPSQQPLTSLPAQPTAQSTSQLQVQALTQQQQSPTKAVPALGKSPPHHSGFQQYQQADASKQLWNPPQVQGPLGKIMPVKQPYYLQTQDPIKLFEPSLQPPVMQQQPLEKKMKPFPMEPYNHNPSEVKVPEFYWDSSYSMADNRSVMAQQANIDRRGKRSPGVFRPEQDPVPRMPFEDPKSSPLLPPDLLKSLAALEEEEELIFSNPPDLYPALLGPLASLPGRSLFKSLLEKPSELMSHSSSFLSLTGFSLNQERYPNNSMFNEVYGKNLTSSSKAELSPSMAPQETSLYSLFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSVPFSNFGPIGTPDNRDRRTADRWKTDKPAMGGFGIDYLSATSSSESSWHQASTPSGTWTGHGPSMEDSSAVLMESLKSIWSSSMMHPGPSALEQLLMQQKQKQQRGQGTMNPPH

Target ORF information:

RefSeq Version	NM_001331007.2
Organism	Homo sapiens(human)
Definition	Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001331007.2

ORF Insert Sequence:

ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
CAGATGAGGA CCGAAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG 
GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCTGA AGTCTGGACA 
TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTAG TTACCGATTT GGAATACGCT 
TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCACGCCT TTAAGAATCA GATCACAACA 
CTGCAAGGCC AGGCAAAGAA TCGAGCAAAT CCGAATCGGA GTGAAGTTCA GGCAAACCTT 
TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA 
GTATTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AATTGGGAAT TATCAGCAAT 
AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC 
CAGCACTGCC TCGTCCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA 
GAGTCCTACT ATAGGCATGC AGCTCAGCTT GTCCCCTCCA ATGGTCAGCC TTATAATCAG 
TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACCA CAATTTTCTA CTACTGCAGA 
AGCATTGCTG TGAAGTTCCC TTTCCCAGCT GCCTCCACTA ATCTGCAAAA AGCACTTTCT 
AAAGCACTGG AAAGCCGAGA TGAGGTGAAA ACCAAGTGGG GTGTTTCTGA CTTCATCAAG 
GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT 
CTTCGAGAGA AATTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAAAAAGC TTTCAACTCT 
CAGCAGTTAG TTCATGTCAC TGTCATTAAC CTGTTTCAAC TTCATCACCT TCGTGACTTT 
AGCAATGAAA CCGAGCAGCA CACTTATAGC CAAGATGAGC AGCTATGTTG GACACAGTTG 
CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTACA GAATGAGTCT 
CAGGAGGAGT CCTACAATGC CTATCCTCTT CCAGCAGTCA AGGTCTCCAT GGACTGGCTA 
AGACTCAGAC CCAGGGTCTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCC 
TGGTTGATTT CTCTTCTGAA TAGTTTCCAT CCCCATGAAG AGGACCTCTC AAGTATTAGT 
GCGACACCAC TTCCAGAGGA GTTTGAATTA CAAGGATTTT TGGCATTGAG ACCTTCTTTC 
AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GGGACAAAGA AGGCCAGCAA 
CGACGAATAC GACAGCAACG CTTGATCTCT ATAGGCAAAT GGATTGCTGA TAATCAGCCA 
AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAA 
TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA AACATCTGTG 
ATAGAGTCGC TGGCTGCAGA TGGGAGCCCA GGGCTAAAAT CAGTGCTATC TACAAGCCGA 
AATTTAAGCA ACAACTGTGA CACAGGAGAG AAGCCAGTGG TTACCTTCAA AGAAAACATT 
AAGACACGAG AAGTGAACAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTAAAATCC 
CAGACAGAAC TAAGAAAGAC TCCAGTGTCT GAAGCCAGAA AAACACCTGT AACTCAAACC 
CCAACTCAAG CAAGTAACTC CCAGTTCATC CCCATTCATC ACCCTGGAGC CTTCCCTCCT 
CTTCCCAGCA GGCCAGGGTT TCCGCCCCCA ACATATGTTA TCCCCCCGCC TGTGGCATTT 
TCTATGGGCT CAGGTTACAC CTTCCCAGCT GGTGTTTCTG TCCCAGGAAC CTTTCTTCAG 
CCTACAGCTC ACTCTCCAGC AGGAAACCAG GTGCAAGCTG GGAAACAGTC CCACATTCCT 
TACAGCCAGC AACGGCCCTC TGGACCAGGG CCAATGAACC AGGGACCTCA ACAATCACAG 
CCACCTTCCC AGCAACCCCT TACATCTTTA CCAGCTCAGC CAACAGCACA GTCTACAAGC 
CAGCTGCAGG TTCAAGCTCT AACTCAGCAA CAACAATCCC CTACAAAAGC TGTGCCGGCT 
TTGGGGAAAA GCCCGCCTCA CCACTCTGGA TTCCAGCAGT ATCAACAGGC AGATGCCTCC 
AAACAGCTGT GGAATCCCCC TCAGGTTCAA GGCCCATTAG GGAAAATTAT GCCTGTGAAA 
CAGCCCTACT ACCTTCAGAC CCAAGACCCC ATAAAACTGT TTGAGCCGTC ATTGCAACCT 
CCTGTAATGC AGCAGCAGCC TCTAGAAAAA AAAATGAAGC CTTTTCCCAT GGAGCCATAT 
AACCATAATC CCTCAGAAGT CAAGGTCCCA GAATTCTACT GGGATTCTTC CTACAGCATG 
GCTGATAACA GATCTGTAAT GGCACAGCAA GCAAACATAG ACCGCAGGGG CAAACGGTCA 
CCAGGAGTCT TCCGTCCAGA GCAGGATCCT GTACCCAGAA TGCCGTTTGA GGACCCCAAG 
AGCTCCCCTC TGCTTCCTCC GGACCTGTTA AAGAGTCTGG CTGCCTTGGA GGAAGAGGAA 
GAGCTGATTT TTTCTAACCC TCCTGATCTT TACCCGGCTC TGCTGGGGCC TCTCGCCTCT 
CTTCCTGGAC GAAGCCTTTT TAAATCCTTA TTGGAGAAGC CCTCAGAGCT CATGTCACAT 
TCATCCTCTT TCCTGTCCCT CACCGGATTC TCTCTCAATC AGGAAAGATA CCCAAATAAT 
AGTATGTTCA ATGAGGTATA TGGGAAAAAC CTGACATCCA GCTCCAAAGC AGAACTCAGT 
CCCTCAATGG CCCCCCAGGA AACATCTCTG TATTCCCTTT TTGAAGGGAC TCCGTGGTCT 
CCATCACTTC CTGCCAGTTC AGATCATTCA ACACCAGCCA GCCAGTCTCC TCATTCCTCT 
AACCCAAGCA GCCTACCCAG CTCTCCTCCA ACACACAACC ATAATTCTGT TCCATTCTCC 
AATTTTGGAC CCATTGGGAC TCCAGATAAC AGGGATAGAA GGACTGCAGA TCGGTGGAAA 
ACTGATAAGC CAGCCATGGG TGGGTTTGGC ATTGATTATC TCTCAGCAAC GTCATCCTCT 
GAGAGCAGTT GGCATCAGGC CAGCACTCCG AGTGGCACCT GGACAGGCCA TGGCCCTTCC 
ATGGAGGATT CCTCTGCTGT CCTCATGGAA AGCCTAAAGT CTATCTGGTC CAGTTCCATG 
ATGCATCCTG GACCTTCTGC TCTGGAGCAG CTGTTAATGC AGCAGAAGCA GAAACAGCAA 
CGGGGACAAG GCACCATGAA CCCTCCACAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG7 ( NM_001350221.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001337150.1 Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 9, mRNA.

Price & Availability↑

CloneID	OHu34883
Clone ID Related Accession (Same CDS sequence)	XM_011510206.2 , NM_001331007.2 , NM_001350221.1 , NM_001331007.1 , NM_001350221.2 , XM_011510206.3
Accession Version	NM_001350221.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3513bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-27
Organism	Homo sapiens(human)
Product	protein SMG7 isoform 6
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB223038.1, AB085674.1, AL137800.12, DC341780.1, D87437.2, DA096344.1 and CN479993.1. On Apr 10, 2017 this sequence version replaced XM_017002966.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.147809.1, SRR1803614.253492.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
CAGATGAGGA CCGAAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG 
GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCTGA AGTCTGGACA 
TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTAG TTACCGATTT GGAATACGCT 
TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCACGCCT TTAAGAATCA GATCACAACA 
CTGCAAGGCC AGGCAAAGAA TCGAGCAAAT CCGAATCGGA GTGAAGTTCA GGCAAACCTT 
TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA 
GTATTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AATTGGGAAT TATCAGCAAT 
AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC 
CAGCACTGCC TCGTCCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA 
GAGTCCTACT ATAGGCATGC AGCTCAGCTT GTCCCCTCCA ATGGTCAGCC TTATAATCAG 
TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACCA CAATTTTCTA CTACTGCAGA 
AGCATTGCTG TGAAGTTCCC TTTCCCAGCT GCCTCCACTA ATCTGCAAAA AGCACTTTCT 
AAAGCACTGG AAAGCCGAGA TGAGGTGAAA ACCAAGTGGG GTGTTTCTGA CTTCATCAAG 
GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT 
CTTCGAGAGA AATTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAAAAAGC TTTCAACTCT 
CAGCAGTTAG TTCATGTCAC TGTCATTAAC CTGTTTCAAC TTCATCACCT TCGTGACTTT 
AGCAATGAAA CCGAGCAGCA CACTTATAGC CAAGATGAGC AGCTATGTTG GACACAGTTG 
CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTACA GAATGAGTCT 
CAGGAGGAGT CCTACAATGC CTATCCTCTT CCAGCAGTCA AGGTCTCCAT GGACTGGCTA 
AGACTCAGAC CCAGGGTCTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCC 
TGGTTGATTT CTCTTCTGAA TAGTTTCCAT CCCCATGAAG AGGACCTCTC AAGTATTAGT 
GCGACACCAC TTCCAGAGGA GTTTGAATTA CAAGGATTTT TGGCATTGAG ACCTTCTTTC 
AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GGGACAAAGA AGGCCAGCAA 
CGACGAATAC GACAGCAACG CTTGATCTCT ATAGGCAAAT GGATTGCTGA TAATCAGCCA 
AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAA 
TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA AACATCTGTG 
ATAGAGTCGC TGGCTGCAGA TGGGAGCCCA GGGCTAAAAT CAGTGCTATC TACAAGCCGA 
AATTTAAGCA ACAACTGTGA CACAGGAGAG AAGCCAGTGG TTACCTTCAA AGAAAACATT 
AAGACACGAG AAGTGAACAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTAAAATCC 
CAGACAGAAC TAAGAAAGAC TCCAGTGTCT GAAGCCAGAA AAACACCTGT AACTCAAACC 
CCAACTCAAG CAAGTAACTC CCAGTTCATC CCCATTCATC ACCCTGGAGC CTTCCCTCCT 
CTTCCCAGCA GGCCAGGGTT TCCGCCCCCA ACATATGTTA TCCCCCCGCC TGTGGCATTT 
TCTATGGGCT CAGGTTACAC CTTCCCAGCT GGTGTTTCTG TCCCAGGAAC CTTTCTTCAG 
CCTACAGCTC ACTCTCCAGC AGGAAACCAG GTGCAAGCTG GGAAACAGTC CCACATTCCT 
TACAGCCAGC AACGGCCCTC TGGACCAGGG CCAATGAACC AGGGACCTCA ACAATCACAG 
CCACCTTCCC AGCAACCCCT TACATCTTTA CCAGCTCAGC CAACAGCACA GTCTACAAGC 
CAGCTGCAGG TTCAAGCTCT AACTCAGCAA CAACAATCCC CTACAAAAGC TGTGCCGGCT 
TTGGGGAAAA GCCCGCCTCA CCACTCTGGA TTCCAGCAGT ATCAACAGGC AGATGCCTCC 
AAACAGCTGT GGAATCCCCC TCAGGTTCAA GGCCCATTAG GGAAAATTAT GCCTGTGAAA 
CAGCCCTACT ACCTTCAGAC CCAAGACCCC ATAAAACTGT TTGAGCCGTC ATTGCAACCT 
CCTGTAATGC AGCAGCAGCC TCTAGAAAAA AAAATGAAGC CTTTTCCCAT GGAGCCATAT 
AACCATAATC CCTCAGAAGT CAAGGTCCCA GAATTCTACT GGGATTCTTC CTACAGCATG 
GCTGATAACA GATCTGTAAT GGCACAGCAA GCAAACATAG ACCGCAGGGG CAAACGGTCA 
CCAGGAGTCT TCCGTCCAGA GCAGGATCCT GTACCCAGAA TGCCGTTTGA GGACCCCAAG 
AGCTCCCCTC TGCTTCCTCC GGACCTGTTA AAGAGTCTGG CTGCCTTGGA GGAAGAGGAA 
GAGCTGATTT TTTCTAACCC TCCTGATCTT TACCCGGCTC TGCTGGGGCC TCTCGCCTCT 
CTTCCTGGAC GAAGCCTTTT TAAATCCTTA TTGGAGAAGC CCTCAGAGCT CATGTCACAT 
TCATCCTCTT TCCTGTCCCT CACCGGATTC TCTCTCAATC AGGAAAGATA CCCAAATAAT 
AGTATGTTCA ATGAGGTATA TGGGAAAAAC CTGACATCCA GCTCCAAAGC AGAACTCAGT 
CCCTCAATGG CCCCCCAGGA AACATCTCTG TATTCCCTTT TTGAAGGGAC TCCGTGGTCT 
CCATCACTTC CTGCCAGTTC AGATCATTCA ACACCAGCCA GCCAGTCTCC TCATTCCTCT 
AACCCAAGCA GCCTACCCAG CTCTCCTCCA ACACACAACC ATAATTCTGT TCCATTCTCC 
AATTTTGGAC CCATTGGGAC TCCAGATAAC AGGGATAGAA GGACTGCAGA TCGGTGGAAA 
ACTGATAAGC CAGCCATGGG TGGGTTTGGC ATTGATTATC TCTCAGCAAC GTCATCCTCT 
GAGAGCAGTT GGCATCAGGC CAGCACTCCG AGTGGCACCT GGACAGGCCA TGGCCCTTCC 
ATGGAGGATT CCTCTGCTGT CCTCATGGAA AGCCTAAAGT CTATCTGGTC CAGTTCCATG 
ATGCATCCTG GACCTTCTGC TCTGGAGCAG CTGTTAATGC AGCAGAAGCA GAAACAGCAA 
CGGGGACAAG GCACCATGAA CCCTCCACAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001337150.1
CDS	440..3952
Translation	MRNWMFLQIVIVGENVSFKSQMRTENLKSEEHLKSSNIRQAEVLKADMTDSKLGPAEVWTSRQALQDLYQKMLVTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRANPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQTHTSAIVKPQSSSCSYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNLQKALSKALESRDEVKTKWGVSDFIKAFIKFHGHVYLSKSLEKLSPLREKLEEQFKRLLFQKAFNSQQLVHVTVINLFQLHHLRDFSNETEQHTYSQDEQLCWTQLLALFMSFLGILCKCPLQNESQEESYNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPHEEDLSSISATPLPEEFELQGFLALRPSFRNLDFSKGHQGITGDKEGQQRRIRQQRLISIGKWIADNQPRLIQCENEVGKLLFITEIPELILEDPSEAKENLILQETSVIESLAADGSPGLKSVLSTSRNLSNNCDTGEKPVVTFKENIKTREVNRDQGRSFPPKEVKSQTELRKTPVSEARKTPVTQTPTQASNSQFIPIHHPGAFPPLPSRPGFPPPTYVIPPPVAFSMGSGYTFPAGVSVPGTFLQPTAHSPAGNQVQAGKQSHIPYSQQRPSGPGPMNQGPQQSQPPSQQPLTSLPAQPTAQSTSQLQVQALTQQQQSPTKAVPALGKSPPHHSGFQQYQQADASKQLWNPPQVQGPLGKIMPVKQPYYLQTQDPIKLFEPSLQPPVMQQQPLEKKMKPFPMEPYNHNPSEVKVPEFYWDSSYSMADNRSVMAQQANIDRRGKRSPGVFRPEQDPVPRMPFEDPKSSPLLPPDLLKSLAALEEEEELIFSNPPDLYPALLGPLASLPGRSLFKSLLEKPSELMSHSSSFLSLTGFSLNQERYPNNSMFNEVYGKNLTSSSKAELSPSMAPQETSLYSLFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSVPFSNFGPIGTPDNRDRRTADRWKTDKPAMGGFGIDYLSATSSSESSWHQASTPSGTWTGHGPSMEDSSAVLMESLKSIWSSSMMHPGPSALEQLLMQQKQKQQRGQGTMNPPH

Target ORF information:

RefSeq Version	NM_001350221.1
Organism	Homo sapiens(human)
Definition	Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001350221.1

ORF Insert Sequence:

ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
CAGATGAGGA CCGAAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG 
GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCTGA AGTCTGGACA 
TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTAG TTACCGATTT GGAATACGCT 
TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCACGCCT TTAAGAATCA GATCACAACA 
CTGCAAGGCC AGGCAAAGAA TCGAGCAAAT CCGAATCGGA GTGAAGTTCA GGCAAACCTT 
TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA 
GTATTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AATTGGGAAT TATCAGCAAT 
AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC 
CAGCACTGCC TCGTCCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA 
GAGTCCTACT ATAGGCATGC AGCTCAGCTT GTCCCCTCCA ATGGTCAGCC TTATAATCAG 
TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACCA CAATTTTCTA CTACTGCAGA 
AGCATTGCTG TGAAGTTCCC TTTCCCAGCT GCCTCCACTA ATCTGCAAAA AGCACTTTCT 
AAAGCACTGG AAAGCCGAGA TGAGGTGAAA ACCAAGTGGG GTGTTTCTGA CTTCATCAAG 
GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT 
CTTCGAGAGA AATTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAAAAAGC TTTCAACTCT 
CAGCAGTTAG TTCATGTCAC TGTCATTAAC CTGTTTCAAC TTCATCACCT TCGTGACTTT 
AGCAATGAAA CCGAGCAGCA CACTTATAGC CAAGATGAGC AGCTATGTTG GACACAGTTG 
CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTACA GAATGAGTCT 
CAGGAGGAGT CCTACAATGC CTATCCTCTT CCAGCAGTCA AGGTCTCCAT GGACTGGCTA 
AGACTCAGAC CCAGGGTCTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCC 
TGGTTGATTT CTCTTCTGAA TAGTTTCCAT CCCCATGAAG AGGACCTCTC AAGTATTAGT 
GCGACACCAC TTCCAGAGGA GTTTGAATTA CAAGGATTTT TGGCATTGAG ACCTTCTTTC 
AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GGGACAAAGA AGGCCAGCAA 
CGACGAATAC GACAGCAACG CTTGATCTCT ATAGGCAAAT GGATTGCTGA TAATCAGCCA 
AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAA 
TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA AACATCTGTG 
ATAGAGTCGC TGGCTGCAGA TGGGAGCCCA GGGCTAAAAT CAGTGCTATC TACAAGCCGA 
AATTTAAGCA ACAACTGTGA CACAGGAGAG AAGCCAGTGG TTACCTTCAA AGAAAACATT 
AAGACACGAG AAGTGAACAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTAAAATCC 
CAGACAGAAC TAAGAAAGAC TCCAGTGTCT GAAGCCAGAA AAACACCTGT AACTCAAACC 
CCAACTCAAG CAAGTAACTC CCAGTTCATC CCCATTCATC ACCCTGGAGC CTTCCCTCCT 
CTTCCCAGCA GGCCAGGGTT TCCGCCCCCA ACATATGTTA TCCCCCCGCC TGTGGCATTT 
TCTATGGGCT CAGGTTACAC CTTCCCAGCT GGTGTTTCTG TCCCAGGAAC CTTTCTTCAG 
CCTACAGCTC ACTCTCCAGC AGGAAACCAG GTGCAAGCTG GGAAACAGTC CCACATTCCT 
TACAGCCAGC AACGGCCCTC TGGACCAGGG CCAATGAACC AGGGACCTCA ACAATCACAG 
CCACCTTCCC AGCAACCCCT TACATCTTTA CCAGCTCAGC CAACAGCACA GTCTACAAGC 
CAGCTGCAGG TTCAAGCTCT AACTCAGCAA CAACAATCCC CTACAAAAGC TGTGCCGGCT 
TTGGGGAAAA GCCCGCCTCA CCACTCTGGA TTCCAGCAGT ATCAACAGGC AGATGCCTCC 
AAACAGCTGT GGAATCCCCC TCAGGTTCAA GGCCCATTAG GGAAAATTAT GCCTGTGAAA 
CAGCCCTACT ACCTTCAGAC CCAAGACCCC ATAAAACTGT TTGAGCCGTC ATTGCAACCT 
CCTGTAATGC AGCAGCAGCC TCTAGAAAAA AAAATGAAGC CTTTTCCCAT GGAGCCATAT 
AACCATAATC CCTCAGAAGT CAAGGTCCCA GAATTCTACT GGGATTCTTC CTACAGCATG 
GCTGATAACA GATCTGTAAT GGCACAGCAA GCAAACATAG ACCGCAGGGG CAAACGGTCA 
CCAGGAGTCT TCCGTCCAGA GCAGGATCCT GTACCCAGAA TGCCGTTTGA GGACCCCAAG 
AGCTCCCCTC TGCTTCCTCC GGACCTGTTA AAGAGTCTGG CTGCCTTGGA GGAAGAGGAA 
GAGCTGATTT TTTCTAACCC TCCTGATCTT TACCCGGCTC TGCTGGGGCC TCTCGCCTCT 
CTTCCTGGAC GAAGCCTTTT TAAATCCTTA TTGGAGAAGC CCTCAGAGCT CATGTCACAT 
TCATCCTCTT TCCTGTCCCT CACCGGATTC TCTCTCAATC AGGAAAGATA CCCAAATAAT 
AGTATGTTCA ATGAGGTATA TGGGAAAAAC CTGACATCCA GCTCCAAAGC AGAACTCAGT 
CCCTCAATGG CCCCCCAGGA AACATCTCTG TATTCCCTTT TTGAAGGGAC TCCGTGGTCT 
CCATCACTTC CTGCCAGTTC AGATCATTCA ACACCAGCCA GCCAGTCTCC TCATTCCTCT 
AACCCAAGCA GCCTACCCAG CTCTCCTCCA ACACACAACC ATAATTCTGT TCCATTCTCC 
AATTTTGGAC CCATTGGGAC TCCAGATAAC AGGGATAGAA GGACTGCAGA TCGGTGGAAA 
ACTGATAAGC CAGCCATGGG TGGGTTTGGC ATTGATTATC TCTCAGCAAC GTCATCCTCT 
GAGAGCAGTT GGCATCAGGC CAGCACTCCG AGTGGCACCT GGACAGGCCA TGGCCCTTCC 
ATGGAGGATT CCTCTGCTGT CCTCATGGAA AGCCTAAAGT CTATCTGGTC CAGTTCCATG 
ATGCATCCTG GACCTTCTGC TCTGGAGCAG CTGTTAATGC AGCAGAAGCA GAAACAGCAA 
CGGGGACAAG GCACCATGAA CCCTCCACAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG7 ( NM_001331007.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317936.1 Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 6, mRNA.

Price & Availability↑

CloneID	OHu34883
Clone ID Related Accession (Same CDS sequence)	XM_011510206.2 , NM_001331007.2 , NM_001350221.1 , NM_001331007.1 , NM_001350221.2 , XM_011510206.3
Accession Version	NM_001331007.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3513bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-27
Organism	Homo sapiens(human)
Product	protein SMG7 isoform 6
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL449223.7 and AL137800.12. On Sep 3, 2016 this sequence version replaced XM_006711676.3. Transcript Variant: This variant (6) differs in the 5' UTR and coding sequence, uses an alternate in-frame splice junction, and contains an alternate in-frame exon compared to variant 1. The resulting isoform (6) has a longer and distinct N-terminus, contains an alternate internal segment, and lacks another alternate internal segment compared to isoform 1. Variants 6 and 9 encode the same protein (isoform 6). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.172321.1, SRR1803611.163447.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END##

ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
CAGATGAGGA CCGAAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG 
GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCTGA AGTCTGGACA 
TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTAG TTACCGATTT GGAATACGCT 
TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCACGCCT TTAAGAATCA GATCACAACA 
CTGCAAGGCC AGGCAAAGAA TCGAGCAAAT CCGAATCGGA GTGAAGTTCA GGCAAACCTT 
TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA 
GTATTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AATTGGGAAT TATCAGCAAT 
AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC 
CAGCACTGCC TCGTCCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA 
GAGTCCTACT ATAGGCATGC AGCTCAGCTT GTCCCCTCCA ATGGTCAGCC TTATAATCAG 
TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACCA CAATTTTCTA CTACTGCAGA 
AGCATTGCTG TGAAGTTCCC TTTCCCAGCT GCCTCCACTA ATCTGCAAAA AGCACTTTCT 
AAAGCACTGG AAAGCCGAGA TGAGGTGAAA ACCAAGTGGG GTGTTTCTGA CTTCATCAAG 
GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT 
CTTCGAGAGA AATTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAAAAAGC TTTCAACTCT 
CAGCAGTTAG TTCATGTCAC TGTCATTAAC CTGTTTCAAC TTCATCACCT TCGTGACTTT 
AGCAATGAAA CCGAGCAGCA CACTTATAGC CAAGATGAGC AGCTATGTTG GACACAGTTG 
CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTACA GAATGAGTCT 
CAGGAGGAGT CCTACAATGC CTATCCTCTT CCAGCAGTCA AGGTCTCCAT GGACTGGCTA 
AGACTCAGAC CCAGGGTCTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCC 
TGGTTGATTT CTCTTCTGAA TAGTTTCCAT CCCCATGAAG AGGACCTCTC AAGTATTAGT 
GCGACACCAC TTCCAGAGGA GTTTGAATTA CAAGGATTTT TGGCATTGAG ACCTTCTTTC 
AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GGGACAAAGA AGGCCAGCAA 
CGACGAATAC GACAGCAACG CTTGATCTCT ATAGGCAAAT GGATTGCTGA TAATCAGCCA 
AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAA 
TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA AACATCTGTG 
ATAGAGTCGC TGGCTGCAGA TGGGAGCCCA GGGCTAAAAT CAGTGCTATC TACAAGCCGA 
AATTTAAGCA ACAACTGTGA CACAGGAGAG AAGCCAGTGG TTACCTTCAA AGAAAACATT 
AAGACACGAG AAGTGAACAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTAAAATCC 
CAGACAGAAC TAAGAAAGAC TCCAGTGTCT GAAGCCAGAA AAACACCTGT AACTCAAACC 
CCAACTCAAG CAAGTAACTC CCAGTTCATC CCCATTCATC ACCCTGGAGC CTTCCCTCCT 
CTTCCCAGCA GGCCAGGGTT TCCGCCCCCA ACATATGTTA TCCCCCCGCC TGTGGCATTT 
TCTATGGGCT CAGGTTACAC CTTCCCAGCT GGTGTTTCTG TCCCAGGAAC CTTTCTTCAG 
CCTACAGCTC ACTCTCCAGC AGGAAACCAG GTGCAAGCTG GGAAACAGTC CCACATTCCT 
TACAGCCAGC AACGGCCCTC TGGACCAGGG CCAATGAACC AGGGACCTCA ACAATCACAG 
CCACCTTCCC AGCAACCCCT TACATCTTTA CCAGCTCAGC CAACAGCACA GTCTACAAGC 
CAGCTGCAGG TTCAAGCTCT AACTCAGCAA CAACAATCCC CTACAAAAGC TGTGCCGGCT 
TTGGGGAAAA GCCCGCCTCA CCACTCTGGA TTCCAGCAGT ATCAACAGGC AGATGCCTCC 
AAACAGCTGT GGAATCCCCC TCAGGTTCAA GGCCCATTAG GGAAAATTAT GCCTGTGAAA 
CAGCCCTACT ACCTTCAGAC CCAAGACCCC ATAAAACTGT TTGAGCCGTC ATTGCAACCT 
CCTGTAATGC AGCAGCAGCC TCTAGAAAAA AAAATGAAGC CTTTTCCCAT GGAGCCATAT 
AACCATAATC CCTCAGAAGT CAAGGTCCCA GAATTCTACT GGGATTCTTC CTACAGCATG 
GCTGATAACA GATCTGTAAT GGCACAGCAA GCAAACATAG ACCGCAGGGG CAAACGGTCA 
CCAGGAGTCT TCCGTCCAGA GCAGGATCCT GTACCCAGAA TGCCGTTTGA GGACCCCAAG 
AGCTCCCCTC TGCTTCCTCC GGACCTGTTA AAGAGTCTGG CTGCCTTGGA GGAAGAGGAA 
GAGCTGATTT TTTCTAACCC TCCTGATCTT TACCCGGCTC TGCTGGGGCC TCTCGCCTCT 
CTTCCTGGAC GAAGCCTTTT TAAATCCTTA TTGGAGAAGC CCTCAGAGCT CATGTCACAT 
TCATCCTCTT TCCTGTCCCT CACCGGATTC TCTCTCAATC AGGAAAGATA CCCAAATAAT 
AGTATGTTCA ATGAGGTATA TGGGAAAAAC CTGACATCCA GCTCCAAAGC AGAACTCAGT 
CCCTCAATGG CCCCCCAGGA AACATCTCTG TATTCCCTTT TTGAAGGGAC TCCGTGGTCT 
CCATCACTTC CTGCCAGTTC AGATCATTCA ACACCAGCCA GCCAGTCTCC TCATTCCTCT 
AACCCAAGCA GCCTACCCAG CTCTCCTCCA ACACACAACC ATAATTCTGT TCCATTCTCC 
AATTTTGGAC CCATTGGGAC TCCAGATAAC AGGGATAGAA GGACTGCAGA TCGGTGGAAA 
ACTGATAAGC CAGCCATGGG TGGGTTTGGC ATTGATTATC TCTCAGCAAC GTCATCCTCT 
GAGAGCAGTT GGCATCAGGC CAGCACTCCG AGTGGCACCT GGACAGGCCA TGGCCCTTCC 
ATGGAGGATT CCTCTGCTGT CCTCATGGAA AGCCTAAAGT CTATCTGGTC CAGTTCCATG 
ATGCATCCTG GACCTTCTGC TCTGGAGCAG CTGTTAATGC AGCAGAAGCA GAAACAGCAA 
CGGGGACAAG GCACCATGAA CCCTCCACAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317936.1
CDS	303..3815
Translation	MRNWMFLQIVIVGENVSFKSQMRTENLKSEEHLKSSNIRQAEVLKADMTDSKLGPAEVWTSRQALQDLYQKMLVTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRANPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQTHTSAIVKPQSSSCSYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNLQKALSKALESRDEVKTKWGVSDFIKAFIKFHGHVYLSKSLEKLSPLREKLEEQFKRLLFQKAFNSQQLVHVTVINLFQLHHLRDFSNETEQHTYSQDEQLCWTQLLALFMSFLGILCKCPLQNESQEESYNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPHEEDLSSISATPLPEEFELQGFLALRPSFRNLDFSKGHQGITGDKEGQQRRIRQQRLISIGKWIADNQPRLIQCENEVGKLLFITEIPELILEDPSEAKENLILQETSVIESLAADGSPGLKSVLSTSRNLSNNCDTGEKPVVTFKENIKTREVNRDQGRSFPPKEVKSQTELRKTPVSEARKTPVTQTPTQASNSQFIPIHHPGAFPPLPSRPGFPPPTYVIPPPVAFSMGSGYTFPAGVSVPGTFLQPTAHSPAGNQVQAGKQSHIPYSQQRPSGPGPMNQGPQQSQPPSQQPLTSLPAQPTAQSTSQLQVQALTQQQQSPTKAVPALGKSPPHHSGFQQYQQADASKQLWNPPQVQGPLGKIMPVKQPYYLQTQDPIKLFEPSLQPPVMQQQPLEKKMKPFPMEPYNHNPSEVKVPEFYWDSSYSMADNRSVMAQQANIDRRGKRSPGVFRPEQDPVPRMPFEDPKSSPLLPPDLLKSLAALEEEEELIFSNPPDLYPALLGPLASLPGRSLFKSLLEKPSELMSHSSSFLSLTGFSLNQERYPNNSMFNEVYGKNLTSSSKAELSPSMAPQETSLYSLFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSVPFSNFGPIGTPDNRDRRTADRWKTDKPAMGGFGIDYLSATSSSESSWHQASTPSGTWTGHGPSMEDSSAVLMESLKSIWSSSMMHPGPSALEQLLMQQKQKQQRGQGTMNPPH

Target ORF information:

RefSeq Version	NM_001331007.1
Organism	Homo sapiens(human)
Definition	Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001331007.1

ORF Insert Sequence:

ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
CAGATGAGGA CCGAAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG 
GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCTGA AGTCTGGACA 
TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTAG TTACCGATTT GGAATACGCT 
TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCACGCCT TTAAGAATCA GATCACAACA 
CTGCAAGGCC AGGCAAAGAA TCGAGCAAAT CCGAATCGGA GTGAAGTTCA GGCAAACCTT 
TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA 
GTATTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AATTGGGAAT TATCAGCAAT 
AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC 
CAGCACTGCC TCGTCCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA 
GAGTCCTACT ATAGGCATGC AGCTCAGCTT GTCCCCTCCA ATGGTCAGCC TTATAATCAG 
TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACCA CAATTTTCTA CTACTGCAGA 
AGCATTGCTG TGAAGTTCCC TTTCCCAGCT GCCTCCACTA ATCTGCAAAA AGCACTTTCT 
AAAGCACTGG AAAGCCGAGA TGAGGTGAAA ACCAAGTGGG GTGTTTCTGA CTTCATCAAG 
GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT 
CTTCGAGAGA AATTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAAAAAGC TTTCAACTCT 
CAGCAGTTAG TTCATGTCAC TGTCATTAAC CTGTTTCAAC TTCATCACCT TCGTGACTTT 
AGCAATGAAA CCGAGCAGCA CACTTATAGC CAAGATGAGC AGCTATGTTG GACACAGTTG 
CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTACA GAATGAGTCT 
CAGGAGGAGT CCTACAATGC CTATCCTCTT CCAGCAGTCA AGGTCTCCAT GGACTGGCTA 
AGACTCAGAC CCAGGGTCTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCC 
TGGTTGATTT CTCTTCTGAA TAGTTTCCAT CCCCATGAAG AGGACCTCTC AAGTATTAGT 
GCGACACCAC TTCCAGAGGA GTTTGAATTA CAAGGATTTT TGGCATTGAG ACCTTCTTTC 
AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GGGACAAAGA AGGCCAGCAA 
CGACGAATAC GACAGCAACG CTTGATCTCT ATAGGCAAAT GGATTGCTGA TAATCAGCCA 
AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAA 
TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA AACATCTGTG 
ATAGAGTCGC TGGCTGCAGA TGGGAGCCCA GGGCTAAAAT CAGTGCTATC TACAAGCCGA 
AATTTAAGCA ACAACTGTGA CACAGGAGAG AAGCCAGTGG TTACCTTCAA AGAAAACATT 
AAGACACGAG AAGTGAACAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTAAAATCC 
CAGACAGAAC TAAGAAAGAC TCCAGTGTCT GAAGCCAGAA AAACACCTGT AACTCAAACC 
CCAACTCAAG CAAGTAACTC CCAGTTCATC CCCATTCATC ACCCTGGAGC CTTCCCTCCT 
CTTCCCAGCA GGCCAGGGTT TCCGCCCCCA ACATATGTTA TCCCCCCGCC TGTGGCATTT 
TCTATGGGCT CAGGTTACAC CTTCCCAGCT GGTGTTTCTG TCCCAGGAAC CTTTCTTCAG 
CCTACAGCTC ACTCTCCAGC AGGAAACCAG GTGCAAGCTG GGAAACAGTC CCACATTCCT 
TACAGCCAGC AACGGCCCTC TGGACCAGGG CCAATGAACC AGGGACCTCA ACAATCACAG 
CCACCTTCCC AGCAACCCCT TACATCTTTA CCAGCTCAGC CAACAGCACA GTCTACAAGC 
CAGCTGCAGG TTCAAGCTCT AACTCAGCAA CAACAATCCC CTACAAAAGC TGTGCCGGCT 
TTGGGGAAAA GCCCGCCTCA CCACTCTGGA TTCCAGCAGT ATCAACAGGC AGATGCCTCC 
AAACAGCTGT GGAATCCCCC TCAGGTTCAA GGCCCATTAG GGAAAATTAT GCCTGTGAAA 
CAGCCCTACT ACCTTCAGAC CCAAGACCCC ATAAAACTGT TTGAGCCGTC ATTGCAACCT 
CCTGTAATGC AGCAGCAGCC TCTAGAAAAA AAAATGAAGC CTTTTCCCAT GGAGCCATAT 
AACCATAATC CCTCAGAAGT CAAGGTCCCA GAATTCTACT GGGATTCTTC CTACAGCATG 
GCTGATAACA GATCTGTAAT GGCACAGCAA GCAAACATAG ACCGCAGGGG CAAACGGTCA 
CCAGGAGTCT TCCGTCCAGA GCAGGATCCT GTACCCAGAA TGCCGTTTGA GGACCCCAAG 
AGCTCCCCTC TGCTTCCTCC GGACCTGTTA AAGAGTCTGG CTGCCTTGGA GGAAGAGGAA 
GAGCTGATTT TTTCTAACCC TCCTGATCTT TACCCGGCTC TGCTGGGGCC TCTCGCCTCT 
CTTCCTGGAC GAAGCCTTTT TAAATCCTTA TTGGAGAAGC CCTCAGAGCT CATGTCACAT 
TCATCCTCTT TCCTGTCCCT CACCGGATTC TCTCTCAATC AGGAAAGATA CCCAAATAAT 
AGTATGTTCA ATGAGGTATA TGGGAAAAAC CTGACATCCA GCTCCAAAGC AGAACTCAGT 
CCCTCAATGG CCCCCCAGGA AACATCTCTG TATTCCCTTT TTGAAGGGAC TCCGTGGTCT 
CCATCACTTC CTGCCAGTTC AGATCATTCA ACACCAGCCA GCCAGTCTCC TCATTCCTCT 
AACCCAAGCA GCCTACCCAG CTCTCCTCCA ACACACAACC ATAATTCTGT TCCATTCTCC 
AATTTTGGAC CCATTGGGAC TCCAGATAAC AGGGATAGAA GGACTGCAGA TCGGTGGAAA 
ACTGATAAGC CAGCCATGGG TGGGTTTGGC ATTGATTATC TCTCAGCAAC GTCATCCTCT 
GAGAGCAGTT GGCATCAGGC CAGCACTCCG AGTGGCACCT GGACAGGCCA TGGCCCTTCC 
ATGGAGGATT CCTCTGCTGT CCTCATGGAA AGCCTAAAGT CTATCTGGTC CAGTTCCATG 
ATGCATCCTG GACCTTCTGC TCTGGAGCAG CTGTTAATGC AGCAGAAGCA GAAACAGCAA 
CGGGGACAAG GCACCATGAA CCCTCCACAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG7 ( NM_001350221.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001337150.1 Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 9, mRNA.

Price & Availability↑

CloneID	OHu34883
Clone ID Related Accession (Same CDS sequence)	XM_011510206.2 , NM_001331007.2 , NM_001350221.1 , NM_001331007.1 , NM_001350221.2 , XM_011510206.3
Accession Version	NM_001350221.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3513bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-28
Organism	Homo sapiens(human)
Product	protein SMG7 isoform 6
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB223038.1, AB085674.1, AL137800.12, DC341780.1, D87437.2, DA096344.1 and CN479993.1. On Jun 2, 2019 this sequence version replaced NM_001350221.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.147809.1, SRR1803614.253492.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
CAGATGAGGA CCGAAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG 
GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCTGA AGTCTGGACA 
TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTAG TTACCGATTT GGAATACGCT 
TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCACGCCT TTAAGAATCA GATCACAACA 
CTGCAAGGCC AGGCAAAGAA TCGAGCAAAT CCGAATCGGA GTGAAGTTCA GGCAAACCTT 
TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA 
GTATTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AATTGGGAAT TATCAGCAAT 
AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC 
CAGCACTGCC TCGTCCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA 
GAGTCCTACT ATAGGCATGC AGCTCAGCTT GTCCCCTCCA ATGGTCAGCC TTATAATCAG 
TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACCA CAATTTTCTA CTACTGCAGA 
AGCATTGCTG TGAAGTTCCC TTTCCCAGCT GCCTCCACTA ATCTGCAAAA AGCACTTTCT 
AAAGCACTGG AAAGCCGAGA TGAGGTGAAA ACCAAGTGGG GTGTTTCTGA CTTCATCAAG 
GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT 
CTTCGAGAGA AATTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAAAAAGC TTTCAACTCT 
CAGCAGTTAG TTCATGTCAC TGTCATTAAC CTGTTTCAAC TTCATCACCT TCGTGACTTT 
AGCAATGAAA CCGAGCAGCA CACTTATAGC CAAGATGAGC AGCTATGTTG GACACAGTTG 
CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTACA GAATGAGTCT 
CAGGAGGAGT CCTACAATGC CTATCCTCTT CCAGCAGTCA AGGTCTCCAT GGACTGGCTA 
AGACTCAGAC CCAGGGTCTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCC 
TGGTTGATTT CTCTTCTGAA TAGTTTCCAT CCCCATGAAG AGGACCTCTC AAGTATTAGT 
GCGACACCAC TTCCAGAGGA GTTTGAATTA CAAGGATTTT TGGCATTGAG ACCTTCTTTC 
AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GGGACAAAGA AGGCCAGCAA 
CGACGAATAC GACAGCAACG CTTGATCTCT ATAGGCAAAT GGATTGCTGA TAATCAGCCA 
AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAA 
TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA AACATCTGTG 
ATAGAGTCGC TGGCTGCAGA TGGGAGCCCA GGGCTAAAAT CAGTGCTATC TACAAGCCGA 
AATTTAAGCA ACAACTGTGA CACAGGAGAG AAGCCAGTGG TTACCTTCAA AGAAAACATT 
AAGACACGAG AAGTGAACAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTAAAATCC 
CAGACAGAAC TAAGAAAGAC TCCAGTGTCT GAAGCCAGAA AAACACCTGT AACTCAAACC 
CCAACTCAAG CAAGTAACTC CCAGTTCATC CCCATTCATC ACCCTGGAGC CTTCCCTCCT 
CTTCCCAGCA GGCCAGGGTT TCCGCCCCCA ACATATGTTA TCCCCCCGCC TGTGGCATTT 
TCTATGGGCT CAGGTTACAC CTTCCCAGCT GGTGTTTCTG TCCCAGGAAC CTTTCTTCAG 
CCTACAGCTC ACTCTCCAGC AGGAAACCAG GTGCAAGCTG GGAAACAGTC CCACATTCCT 
TACAGCCAGC AACGGCCCTC TGGACCAGGG CCAATGAACC AGGGACCTCA ACAATCACAG 
CCACCTTCCC AGCAACCCCT TACATCTTTA CCAGCTCAGC CAACAGCACA GTCTACAAGC 
CAGCTGCAGG TTCAAGCTCT AACTCAGCAA CAACAATCCC CTACAAAAGC TGTGCCGGCT 
TTGGGGAAAA GCCCGCCTCA CCACTCTGGA TTCCAGCAGT ATCAACAGGC AGATGCCTCC 
AAACAGCTGT GGAATCCCCC TCAGGTTCAA GGCCCATTAG GGAAAATTAT GCCTGTGAAA 
CAGCCCTACT ACCTTCAGAC CCAAGACCCC ATAAAACTGT TTGAGCCGTC ATTGCAACCT 
CCTGTAATGC AGCAGCAGCC TCTAGAAAAA AAAATGAAGC CTTTTCCCAT GGAGCCATAT 
AACCATAATC CCTCAGAAGT CAAGGTCCCA GAATTCTACT GGGATTCTTC CTACAGCATG 
GCTGATAACA GATCTGTAAT GGCACAGCAA GCAAACATAG ACCGCAGGGG CAAACGGTCA 
CCAGGAGTCT TCCGTCCAGA GCAGGATCCT GTACCCAGAA TGCCGTTTGA GGACCCCAAG 
AGCTCCCCTC TGCTTCCTCC GGACCTGTTA AAGAGTCTGG CTGCCTTGGA GGAAGAGGAA 
GAGCTGATTT TTTCTAACCC TCCTGATCTT TACCCGGCTC TGCTGGGGCC TCTCGCCTCT 
CTTCCTGGAC GAAGCCTTTT TAAATCCTTA TTGGAGAAGC CCTCAGAGCT CATGTCACAT 
TCATCCTCTT TCCTGTCCCT CACCGGATTC TCTCTCAATC AGGAAAGATA CCCAAATAAT 
AGTATGTTCA ATGAGGTATA TGGGAAAAAC CTGACATCCA GCTCCAAAGC AGAACTCAGT 
CCCTCAATGG CCCCCCAGGA AACATCTCTG TATTCCCTTT TTGAAGGGAC TCCGTGGTCT 
CCATCACTTC CTGCCAGTTC AGATCATTCA ACACCAGCCA GCCAGTCTCC TCATTCCTCT 
AACCCAAGCA GCCTACCCAG CTCTCCTCCA ACACACAACC ATAATTCTGT TCCATTCTCC 
AATTTTGGAC CCATTGGGAC TCCAGATAAC AGGGATAGAA GGACTGCAGA TCGGTGGAAA 
ACTGATAAGC CAGCCATGGG TGGGTTTGGC ATTGATTATC TCTCAGCAAC GTCATCCTCT 
GAGAGCAGTT GGCATCAGGC CAGCACTCCG AGTGGCACCT GGACAGGCCA TGGCCCTTCC 
ATGGAGGATT CCTCTGCTGT CCTCATGGAA AGCCTAAAGT CTATCTGGTC CAGTTCCATG 
ATGCATCCTG GACCTTCTGC TCTGGAGCAG CTGTTAATGC AGCAGAAGCA GAAACAGCAA 
CGGGGACAAG GCACCATGAA CCCTCCACAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001337150.1
CDS	312..3824
Translation	MRNWMFLQIVIVGENVSFKSQMRTENLKSEEHLKSSNIRQAEVLKADMTDSKLGPAEVWTSRQALQDLYQKMLVTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRANPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQTHTSAIVKPQSSSCSYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNLQKALSKALESRDEVKTKWGVSDFIKAFIKFHGHVYLSKSLEKLSPLREKLEEQFKRLLFQKAFNSQQLVHVTVINLFQLHHLRDFSNETEQHTYSQDEQLCWTQLLALFMSFLGILCKCPLQNESQEESYNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPHEEDLSSISATPLPEEFELQGFLALRPSFRNLDFSKGHQGITGDKEGQQRRIRQQRLISIGKWIADNQPRLIQCENEVGKLLFITEIPELILEDPSEAKENLILQETSVIESLAADGSPGLKSVLSTSRNLSNNCDTGEKPVVTFKENIKTREVNRDQGRSFPPKEVKSQTELRKTPVSEARKTPVTQTPTQASNSQFIPIHHPGAFPPLPSRPGFPPPTYVIPPPVAFSMGSGYTFPAGVSVPGTFLQPTAHSPAGNQVQAGKQSHIPYSQQRPSGPGPMNQGPQQSQPPSQQPLTSLPAQPTAQSTSQLQVQALTQQQQSPTKAVPALGKSPPHHSGFQQYQQADASKQLWNPPQVQGPLGKIMPVKQPYYLQTQDPIKLFEPSLQPPVMQQQPLEKKMKPFPMEPYNHNPSEVKVPEFYWDSSYSMADNRSVMAQQANIDRRGKRSPGVFRPEQDPVPRMPFEDPKSSPLLPPDLLKSLAALEEEEELIFSNPPDLYPALLGPLASLPGRSLFKSLLEKPSELMSHSSSFLSLTGFSLNQERYPNNSMFNEVYGKNLTSSSKAELSPSMAPQETSLYSLFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSVPFSNFGPIGTPDNRDRRTADRWKTDKPAMGGFGIDYLSATSSSESSWHQASTPSGTWTGHGPSMEDSSAVLMESLKSIWSSSMMHPGPSALEQLLMQQKQKQQRGQGTMNPPH

Target ORF information:

RefSeq Version	NM_001350221.2
Organism	Homo sapiens(human)
Definition	Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001350221.2

ORF Insert Sequence:

ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
CAGATGAGGA CCGAAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG 
GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCTGA AGTCTGGACA 
TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTAG TTACCGATTT GGAATACGCT 
TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCACGCCT TTAAGAATCA GATCACAACA 
CTGCAAGGCC AGGCAAAGAA TCGAGCAAAT CCGAATCGGA GTGAAGTTCA GGCAAACCTT 
TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA 
GTATTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AATTGGGAAT TATCAGCAAT 
AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC 
CAGCACTGCC TCGTCCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA 
GAGTCCTACT ATAGGCATGC AGCTCAGCTT GTCCCCTCCA ATGGTCAGCC TTATAATCAG 
TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACCA CAATTTTCTA CTACTGCAGA 
AGCATTGCTG TGAAGTTCCC TTTCCCAGCT GCCTCCACTA ATCTGCAAAA AGCACTTTCT 
AAAGCACTGG AAAGCCGAGA TGAGGTGAAA ACCAAGTGGG GTGTTTCTGA CTTCATCAAG 
GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT 
CTTCGAGAGA AATTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAAAAAGC TTTCAACTCT 
CAGCAGTTAG TTCATGTCAC TGTCATTAAC CTGTTTCAAC TTCATCACCT TCGTGACTTT 
AGCAATGAAA CCGAGCAGCA CACTTATAGC CAAGATGAGC AGCTATGTTG GACACAGTTG 
CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTACA GAATGAGTCT 
CAGGAGGAGT CCTACAATGC CTATCCTCTT CCAGCAGTCA AGGTCTCCAT GGACTGGCTA 
AGACTCAGAC CCAGGGTCTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCC 
TGGTTGATTT CTCTTCTGAA TAGTTTCCAT CCCCATGAAG AGGACCTCTC AAGTATTAGT 
GCGACACCAC TTCCAGAGGA GTTTGAATTA CAAGGATTTT TGGCATTGAG ACCTTCTTTC 
AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GGGACAAAGA AGGCCAGCAA 
CGACGAATAC GACAGCAACG CTTGATCTCT ATAGGCAAAT GGATTGCTGA TAATCAGCCA 
AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAA 
TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA AACATCTGTG 
ATAGAGTCGC TGGCTGCAGA TGGGAGCCCA GGGCTAAAAT CAGTGCTATC TACAAGCCGA 
AATTTAAGCA ACAACTGTGA CACAGGAGAG AAGCCAGTGG TTACCTTCAA AGAAAACATT 
AAGACACGAG AAGTGAACAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTAAAATCC 
CAGACAGAAC TAAGAAAGAC TCCAGTGTCT GAAGCCAGAA AAACACCTGT AACTCAAACC 
CCAACTCAAG CAAGTAACTC CCAGTTCATC CCCATTCATC ACCCTGGAGC CTTCCCTCCT 
CTTCCCAGCA GGCCAGGGTT TCCGCCCCCA ACATATGTTA TCCCCCCGCC TGTGGCATTT 
TCTATGGGCT CAGGTTACAC CTTCCCAGCT GGTGTTTCTG TCCCAGGAAC CTTTCTTCAG 
CCTACAGCTC ACTCTCCAGC AGGAAACCAG GTGCAAGCTG GGAAACAGTC CCACATTCCT 
TACAGCCAGC AACGGCCCTC TGGACCAGGG CCAATGAACC AGGGACCTCA ACAATCACAG 
CCACCTTCCC AGCAACCCCT TACATCTTTA CCAGCTCAGC CAACAGCACA GTCTACAAGC 
CAGCTGCAGG TTCAAGCTCT AACTCAGCAA CAACAATCCC CTACAAAAGC TGTGCCGGCT 
TTGGGGAAAA GCCCGCCTCA CCACTCTGGA TTCCAGCAGT ATCAACAGGC AGATGCCTCC 
AAACAGCTGT GGAATCCCCC TCAGGTTCAA GGCCCATTAG GGAAAATTAT GCCTGTGAAA 
CAGCCCTACT ACCTTCAGAC CCAAGACCCC ATAAAACTGT TTGAGCCGTC ATTGCAACCT 
CCTGTAATGC AGCAGCAGCC TCTAGAAAAA AAAATGAAGC CTTTTCCCAT GGAGCCATAT 
AACCATAATC CCTCAGAAGT CAAGGTCCCA GAATTCTACT GGGATTCTTC CTACAGCATG 
GCTGATAACA GATCTGTAAT GGCACAGCAA GCAAACATAG ACCGCAGGGG CAAACGGTCA 
CCAGGAGTCT TCCGTCCAGA GCAGGATCCT GTACCCAGAA TGCCGTTTGA GGACCCCAAG 
AGCTCCCCTC TGCTTCCTCC GGACCTGTTA AAGAGTCTGG CTGCCTTGGA GGAAGAGGAA 
GAGCTGATTT TTTCTAACCC TCCTGATCTT TACCCGGCTC TGCTGGGGCC TCTCGCCTCT 
CTTCCTGGAC GAAGCCTTTT TAAATCCTTA TTGGAGAAGC CCTCAGAGCT CATGTCACAT 
TCATCCTCTT TCCTGTCCCT CACCGGATTC TCTCTCAATC AGGAAAGATA CCCAAATAAT 
AGTATGTTCA ATGAGGTATA TGGGAAAAAC CTGACATCCA GCTCCAAAGC AGAACTCAGT 
CCCTCAATGG CCCCCCAGGA AACATCTCTG TATTCCCTTT TTGAAGGGAC TCCGTGGTCT 
CCATCACTTC CTGCCAGTTC AGATCATTCA ACACCAGCCA GCCAGTCTCC TCATTCCTCT 
AACCCAAGCA GCCTACCCAG CTCTCCTCCA ACACACAACC ATAATTCTGT TCCATTCTCC 
AATTTTGGAC CCATTGGGAC TCCAGATAAC AGGGATAGAA GGACTGCAGA TCGGTGGAAA 
ACTGATAAGC CAGCCATGGG TGGGTTTGGC ATTGATTATC TCTCAGCAAC GTCATCCTCT 
GAGAGCAGTT GGCATCAGGC CAGCACTCCG AGTGGCACCT GGACAGGCCA TGGCCCTTCC 
ATGGAGGATT CCTCTGCTGT CCTCATGGAA AGCCTAAAGT CTATCTGGTC CAGTTCCATG 
ATGCATCCTG GACCTTCTGC TCTGGAGCAG CTGTTAATGC AGCAGAAGCA GAAACAGCAA 
CGGGGACAAG GCACCATGAA CCCTCCACAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG7 ( NM_001350219.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001337148.1 Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 7, mRNA.

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CloneID	OHu63455
Clone ID Related Accession (Same CDS sequence)	NM_001350219.1 , NM_001350219.2 , XM_011510209.3 , XM_011510209.2
Accession Version	NM_001350219.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3363bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-27
Organism	Homo sapiens(human)
Product	protein SMG7 isoform 7
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB223038.1, AB085674.1, AL137800.12, DC341780.1, D87437.2 and CN479993.1. On Apr 10, 2017 this sequence version replaced XM_017002967.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.13416.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
CAGATGAGGA CCGAAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG 
GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCTGA AGTCTGGACA 
TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTAG TTACCGATTT GGAATACGCT 
TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCACGCCT TTAAGAATCA GATCACAACA 
CTGCAAGGCC AGGCAAAGAA TCGAGCAAAT CCGAATCGGA GTGAAGTTCA GGCAAACCTT 
TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA 
GTATTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AATTGGGAAT TATCAGCAAT 
AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC 
CAGCACTGCC TCGTCCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA 
GAGTCCTACT ATAGGCATGC AGCTCAGCTT GTCCCCTCCA ATGGTCAGCC TTATAATCAG 
TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACCA CAATTTTCTA CTACTGCAGA 
AGCATTGCTG TGAAGTTCCC TTTCCCAGCT GCCTCCACTA ATCTGCAAAA AGCACTTTCT 
AAAGCACTGG AAAGCCGAGA TGAGGTGAAA ACCAAGTGGG GTGTTTCTGA CTTCATCAAG 
GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT 
CTTCGAGAGA AATTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAAAAAGC TTTCAACTCT 
CAGCAGTTAG TTCATGTCAC TGTCATTAAC CTGTTTCAAC TTCATCACCT TCGTGACTTT 
AGCAATGAAA CCGAGCAGCA CACTTATAGC CAAGATGAGC AGCTATGTTG GACACAGTTG 
CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTACA GAATGAGTCT 
CAGGAGGAGT CCTACAATGC CTATCCTCTT CCAGCAGTCA AGGTCTCCAT GGACTGGCTA 
AGACTCAGAC CCAGGGTCTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCC 
TGGTTGATTT CTCTTCTGAA TAGTTTCCAT CCCCATGAAG AGGACCTCTC AAGTATTAGT 
GCGACACCAC TTCCAGAGGA GTTTGAATTA CAAGGATTTT TGGCATTGAG ACCTTCTTTC 
AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GGGACAAAGA AGGCCAGCAA 
CGACGAATAC GACAGCAACG CTTGATCTCT ATAGGCAAAT GGATTGCTGA TAATCAGCCA 
AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAA 
TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA AACATCTGTG 
ATAGAGTCGC TGGCTGCAGA TGGGAGCCCA GGGCTAAAAT CAGTGCTATC TACAAGCCGA 
AATTTAAGCA ACAACTGTGA CACAGGAGAG AAGCCAGTGG TTACCTTCAA AGAAAACATT 
AAGACACGAG AAGTGAACAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTAAAATCC 
CAGACAGAAC TAAGAAAGAC TCCAGTGTCT GAAGCCAGAA AAACACCTGT AACTCAAACC 
CCAACTCAAG CAAGTAACTC CCAGTTCATC CCCATTCATC ACCCTGGAGC CTTCCCTCCT 
CTTCCCAGCA GGCCAGGGTT TCCGCCCCCA ACATATGTTA TCCCCCCGCC TGTGGCATTT 
TCTATGGGCT CAGGTTACAC CTTCCCAGCT GGTGTTTCTG TCCCAGGAAC CTTTCTTCAG 
CCTACAGCTC ACTCTCCAGC AGGAAACCAG GTGCAAGCTG GGAAACAGTC CCACATTCCT 
TACAGCCAGC AACGGCCCTC TGGACCAGGG CCAATGAACC AGGGACCTCA ACAATCACAG 
CCACCTTCCC AGCAACCCCT TACATCTTTA CCAGCTCAGC CAACAGCACA GTCTACAAGC 
CAGCTGCAGG TTCAAGCTCT AACTCAGCAA CAACAATCCC CTACAAAAGC TGTGCCGGCT 
TTGGGGAAAA GCCCGCCTCA CCACTCTGGA TTCCAGCAGT ATCAACAGGC AGATGCCTCC 
AAACAGCTGT GGAATCCCCC TCAGGTTCAA GGCCCATTAG GGAAAATTAT GCCTGTGAAA 
CAGCCCTACT ACCTTCAGAC CCAAGACCCC ATAAAACTGT TTGAGCCGTC ATTGCAACCT 
CCTGTAATGC AGCAGCAGCC TCTAGAAAAA AAAATGAAGC CTTTTCCCAT GGAGCCATAT 
AACCATAATC CCTCAGAAGT CAAGGTCCCA GAATTCTACT GGGATTCTTC CTACAGCATG 
GCTGATAACA GATCTGTAAT GGCACAGCAA GCAAACATAG ACCGCAGGGG CAAACGGTCA 
CCAGGAGTCT TCCGTCCAGA GCAGGATCCT GTACCCAGAA TGCCGTTTGA GAAATCCTTA 
TTGGAGAAGC CCTCAGAGCT CATGTCACAT TCATCCTCTT TCCTGTCCCT CACCGGATTC 
TCTCTCAATC AGGAAAGATA CCCAAATAAT AGTATGTTCA ATGAGGTATA TGGGAAAAAC 
CTGACATCCA GCTCCAAAGC AGAACTCAGT CCCTCAATGG CCCCCCAGGA AACATCTCTG 
TATTCCCTTT TTGAAGGGAC TCCGTGGTCT CCATCACTTC CTGCCAGTTC AGATCATTCA 
ACACCAGCCA GCCAGTCTCC TCATTCCTCT AACCCAAGCA GCCTACCCAG CTCTCCTCCA 
ACACACAACC ATAATTCTGT TCCATTCTCC AATTTTGGAC CCATTGGGAC TCCAGATAAC 
AGGGATAGAA GGACTGCAGA TCGGTGGAAA ACTGATAAGC CAGCCATGGG TGGGTTTGGC 
ATTGATTATC TCTCAGCAAC GTCATCCTCT GAGAGCAGTT GGCATCAGGC CAGCACTCCG 
AGTGGCACCT GGACAGGCCA TGGCCCTTCC ATGGAGGATT CCTCTGCTGT CCTCATGGAA 
AGCCTAAAGT CTATCTGGTC CAGTTCCATG ATGCATCCTG GACCTTCTGC TCTGGAGCAG 
CTGTTAATGC AGCAGAAGCA GAAACAGCAA CGGGGACAAG GCACCATGAA CCCTCCACAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001337148.1
CDS	440..3802
Translation	MRNWMFLQIVIVGENVSFKSQMRTENLKSEEHLKSSNIRQAEVLKADMTDSKLGPAEVWTSRQALQDLYQKMLVTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRANPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQTHTSAIVKPQSSSCSYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNLQKALSKALESRDEVKTKWGVSDFIKAFIKFHGHVYLSKSLEKLSPLREKLEEQFKRLLFQKAFNSQQLVHVTVINLFQLHHLRDFSNETEQHTYSQDEQLCWTQLLALFMSFLGILCKCPLQNESQEESYNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPHEEDLSSISATPLPEEFELQGFLALRPSFRNLDFSKGHQGITGDKEGQQRRIRQQRLISIGKWIADNQPRLIQCENEVGKLLFITEIPELILEDPSEAKENLILQETSVIESLAADGSPGLKSVLSTSRNLSNNCDTGEKPVVTFKENIKTREVNRDQGRSFPPKEVKSQTELRKTPVSEARKTPVTQTPTQASNSQFIPIHHPGAFPPLPSRPGFPPPTYVIPPPVAFSMGSGYTFPAGVSVPGTFLQPTAHSPAGNQVQAGKQSHIPYSQQRPSGPGPMNQGPQQSQPPSQQPLTSLPAQPTAQSTSQLQVQALTQQQQSPTKAVPALGKSPPHHSGFQQYQQADASKQLWNPPQVQGPLGKIMPVKQPYYLQTQDPIKLFEPSLQPPVMQQQPLEKKMKPFPMEPYNHNPSEVKVPEFYWDSSYSMADNRSVMAQQANIDRRGKRSPGVFRPEQDPVPRMPFEKSLLEKPSELMSHSSSFLSLTGFSLNQERYPNNSMFNEVYGKNLTSSSKAELSPSMAPQETSLYSLFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSVPFSNFGPIGTPDNRDRRTADRWKTDKPAMGGFGIDYLSATSSSESSWHQASTPSGTWTGHGPSMEDSSAVLMESLKSIWSSSMMHPGPSALEQLLMQQKQKQQRGQGTMNPPH

Target ORF information:

RefSeq Version	NM_001350219.1
Organism	Homo sapiens(human)
Definition	Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001350219.1

ORF Insert Sequence:

ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
CAGATGAGGA CCGAAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG 
GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCTGA AGTCTGGACA 
TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTAG TTACCGATTT GGAATACGCT 
TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCACGCCT TTAAGAATCA GATCACAACA 
CTGCAAGGCC AGGCAAAGAA TCGAGCAAAT CCGAATCGGA GTGAAGTTCA GGCAAACCTT 
TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA 
GTATTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AATTGGGAAT TATCAGCAAT 
AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC 
CAGCACTGCC TCGTCCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA 
GAGTCCTACT ATAGGCATGC AGCTCAGCTT GTCCCCTCCA ATGGTCAGCC TTATAATCAG 
TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACCA CAATTTTCTA CTACTGCAGA 
AGCATTGCTG TGAAGTTCCC TTTCCCAGCT GCCTCCACTA ATCTGCAAAA AGCACTTTCT 
AAAGCACTGG AAAGCCGAGA TGAGGTGAAA ACCAAGTGGG GTGTTTCTGA CTTCATCAAG 
GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT 
CTTCGAGAGA AATTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAAAAAGC TTTCAACTCT 
CAGCAGTTAG TTCATGTCAC TGTCATTAAC CTGTTTCAAC TTCATCACCT TCGTGACTTT 
AGCAATGAAA CCGAGCAGCA CACTTATAGC CAAGATGAGC AGCTATGTTG GACACAGTTG 
CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTACA GAATGAGTCT 
CAGGAGGAGT CCTACAATGC CTATCCTCTT CCAGCAGTCA AGGTCTCCAT GGACTGGCTA 
AGACTCAGAC CCAGGGTCTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCC 
TGGTTGATTT CTCTTCTGAA TAGTTTCCAT CCCCATGAAG AGGACCTCTC AAGTATTAGT 
GCGACACCAC TTCCAGAGGA GTTTGAATTA CAAGGATTTT TGGCATTGAG ACCTTCTTTC 
AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GGGACAAAGA AGGCCAGCAA 
CGACGAATAC GACAGCAACG CTTGATCTCT ATAGGCAAAT GGATTGCTGA TAATCAGCCA 
AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAA 
TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA AACATCTGTG 
ATAGAGTCGC TGGCTGCAGA TGGGAGCCCA GGGCTAAAAT CAGTGCTATC TACAAGCCGA 
AATTTAAGCA ACAACTGTGA CACAGGAGAG AAGCCAGTGG TTACCTTCAA AGAAAACATT 
AAGACACGAG AAGTGAACAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTAAAATCC 
CAGACAGAAC TAAGAAAGAC TCCAGTGTCT GAAGCCAGAA AAACACCTGT AACTCAAACC 
CCAACTCAAG CAAGTAACTC CCAGTTCATC CCCATTCATC ACCCTGGAGC CTTCCCTCCT 
CTTCCCAGCA GGCCAGGGTT TCCGCCCCCA ACATATGTTA TCCCCCCGCC TGTGGCATTT 
TCTATGGGCT CAGGTTACAC CTTCCCAGCT GGTGTTTCTG TCCCAGGAAC CTTTCTTCAG 
CCTACAGCTC ACTCTCCAGC AGGAAACCAG GTGCAAGCTG GGAAACAGTC CCACATTCCT 
TACAGCCAGC AACGGCCCTC TGGACCAGGG CCAATGAACC AGGGACCTCA ACAATCACAG 
CCACCTTCCC AGCAACCCCT TACATCTTTA CCAGCTCAGC CAACAGCACA GTCTACAAGC 
CAGCTGCAGG TTCAAGCTCT AACTCAGCAA CAACAATCCC CTACAAAAGC TGTGCCGGCT 
TTGGGGAAAA GCCCGCCTCA CCACTCTGGA TTCCAGCAGT ATCAACAGGC AGATGCCTCC 
AAACAGCTGT GGAATCCCCC TCAGGTTCAA GGCCCATTAG GGAAAATTAT GCCTGTGAAA 
CAGCCCTACT ACCTTCAGAC CCAAGACCCC ATAAAACTGT TTGAGCCGTC ATTGCAACCT 
CCTGTAATGC AGCAGCAGCC TCTAGAAAAA AAAATGAAGC CTTTTCCCAT GGAGCCATAT 
AACCATAATC CCTCAGAAGT CAAGGTCCCA GAATTCTACT GGGATTCTTC CTACAGCATG 
GCTGATAACA GATCTGTAAT GGCACAGCAA GCAAACATAG ACCGCAGGGG CAAACGGTCA 
CCAGGAGTCT TCCGTCCAGA GCAGGATCCT GTACCCAGAA TGCCGTTTGA GAAATCCTTA 
TTGGAGAAGC CCTCAGAGCT CATGTCACAT TCATCCTCTT TCCTGTCCCT CACCGGATTC 
TCTCTCAATC AGGAAAGATA CCCAAATAAT AGTATGTTCA ATGAGGTATA TGGGAAAAAC 
CTGACATCCA GCTCCAAAGC AGAACTCAGT CCCTCAATGG CCCCCCAGGA AACATCTCTG 
TATTCCCTTT TTGAAGGGAC TCCGTGGTCT CCATCACTTC CTGCCAGTTC AGATCATTCA 
ACACCAGCCA GCCAGTCTCC TCATTCCTCT AACCCAAGCA GCCTACCCAG CTCTCCTCCA 
ACACACAACC ATAATTCTGT TCCATTCTCC AATTTTGGAC CCATTGGGAC TCCAGATAAC 
AGGGATAGAA GGACTGCAGA TCGGTGGAAA ACTGATAAGC CAGCCATGGG TGGGTTTGGC 
ATTGATTATC TCTCAGCAAC GTCATCCTCT GAGAGCAGTT GGCATCAGGC CAGCACTCCG 
AGTGGCACCT GGACAGGCCA TGGCCCTTCC ATGGAGGATT CCTCTGCTGT CCTCATGGAA 
AGCCTAAAGT CTATCTGGTC CAGTTCCATG ATGCATCCTG GACCTTCTGC TCTGGAGCAG 
CTGTTAATGC AGCAGAAGCA GAAACAGCAA CGGGGACAAG GCACCATGAA CCCTCCACAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG7 ( NM_001350219.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001337148.1 Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 7, mRNA.

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CloneID	OHu63455
Clone ID Related Accession (Same CDS sequence)	NM_001350219.1 , NM_001350219.2 , XM_011510209.3 , XM_011510209.2
Accession Version	NM_001350219.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3363bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-29
Organism	Homo sapiens(human)
Product	protein SMG7 isoform 7
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB223038.1, AB085674.1, AL137800.12, DC341780.1, D87437.2 and CN479993.1. On Jun 2, 2019 this sequence version replaced NM_001350219.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.13416.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
CAGATGAGGA CCGAAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG 
GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCTGA AGTCTGGACA 
TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTAG TTACCGATTT GGAATACGCT 
TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCACGCCT TTAAGAATCA GATCACAACA 
CTGCAAGGCC AGGCAAAGAA TCGAGCAAAT CCGAATCGGA GTGAAGTTCA GGCAAACCTT 
TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA 
GTATTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AATTGGGAAT TATCAGCAAT 
AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC 
CAGCACTGCC TCGTCCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA 
GAGTCCTACT ATAGGCATGC AGCTCAGCTT GTCCCCTCCA ATGGTCAGCC TTATAATCAG 
TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACCA CAATTTTCTA CTACTGCAGA 
AGCATTGCTG TGAAGTTCCC TTTCCCAGCT GCCTCCACTA ATCTGCAAAA AGCACTTTCT 
AAAGCACTGG AAAGCCGAGA TGAGGTGAAA ACCAAGTGGG GTGTTTCTGA CTTCATCAAG 
GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT 
CTTCGAGAGA AATTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAAAAAGC TTTCAACTCT 
CAGCAGTTAG TTCATGTCAC TGTCATTAAC CTGTTTCAAC TTCATCACCT TCGTGACTTT 
AGCAATGAAA CCGAGCAGCA CACTTATAGC CAAGATGAGC AGCTATGTTG GACACAGTTG 
CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTACA GAATGAGTCT 
CAGGAGGAGT CCTACAATGC CTATCCTCTT CCAGCAGTCA AGGTCTCCAT GGACTGGCTA 
AGACTCAGAC CCAGGGTCTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCC 
TGGTTGATTT CTCTTCTGAA TAGTTTCCAT CCCCATGAAG AGGACCTCTC AAGTATTAGT 
GCGACACCAC TTCCAGAGGA GTTTGAATTA CAAGGATTTT TGGCATTGAG ACCTTCTTTC 
AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GGGACAAAGA AGGCCAGCAA 
CGACGAATAC GACAGCAACG CTTGATCTCT ATAGGCAAAT GGATTGCTGA TAATCAGCCA 
AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAA 
TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA AACATCTGTG 
ATAGAGTCGC TGGCTGCAGA TGGGAGCCCA GGGCTAAAAT CAGTGCTATC TACAAGCCGA 
AATTTAAGCA ACAACTGTGA CACAGGAGAG AAGCCAGTGG TTACCTTCAA AGAAAACATT 
AAGACACGAG AAGTGAACAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTAAAATCC 
CAGACAGAAC TAAGAAAGAC TCCAGTGTCT GAAGCCAGAA AAACACCTGT AACTCAAACC 
CCAACTCAAG CAAGTAACTC CCAGTTCATC CCCATTCATC ACCCTGGAGC CTTCCCTCCT 
CTTCCCAGCA GGCCAGGGTT TCCGCCCCCA ACATATGTTA TCCCCCCGCC TGTGGCATTT 
TCTATGGGCT CAGGTTACAC CTTCCCAGCT GGTGTTTCTG TCCCAGGAAC CTTTCTTCAG 
CCTACAGCTC ACTCTCCAGC AGGAAACCAG GTGCAAGCTG GGAAACAGTC CCACATTCCT 
TACAGCCAGC AACGGCCCTC TGGACCAGGG CCAATGAACC AGGGACCTCA ACAATCACAG 
CCACCTTCCC AGCAACCCCT TACATCTTTA CCAGCTCAGC CAACAGCACA GTCTACAAGC 
CAGCTGCAGG TTCAAGCTCT AACTCAGCAA CAACAATCCC CTACAAAAGC TGTGCCGGCT 
TTGGGGAAAA GCCCGCCTCA CCACTCTGGA TTCCAGCAGT ATCAACAGGC AGATGCCTCC 
AAACAGCTGT GGAATCCCCC TCAGGTTCAA GGCCCATTAG GGAAAATTAT GCCTGTGAAA 
CAGCCCTACT ACCTTCAGAC CCAAGACCCC ATAAAACTGT TTGAGCCGTC ATTGCAACCT 
CCTGTAATGC AGCAGCAGCC TCTAGAAAAA AAAATGAAGC CTTTTCCCAT GGAGCCATAT 
AACCATAATC CCTCAGAAGT CAAGGTCCCA GAATTCTACT GGGATTCTTC CTACAGCATG 
GCTGATAACA GATCTGTAAT GGCACAGCAA GCAAACATAG ACCGCAGGGG CAAACGGTCA 
CCAGGAGTCT TCCGTCCAGA GCAGGATCCT GTACCCAGAA TGCCGTTTGA GAAATCCTTA 
TTGGAGAAGC CCTCAGAGCT CATGTCACAT TCATCCTCTT TCCTGTCCCT CACCGGATTC 
TCTCTCAATC AGGAAAGATA CCCAAATAAT AGTATGTTCA ATGAGGTATA TGGGAAAAAC 
CTGACATCCA GCTCCAAAGC AGAACTCAGT CCCTCAATGG CCCCCCAGGA AACATCTCTG 
TATTCCCTTT TTGAAGGGAC TCCGTGGTCT CCATCACTTC CTGCCAGTTC AGATCATTCA 
ACACCAGCCA GCCAGTCTCC TCATTCCTCT AACCCAAGCA GCCTACCCAG CTCTCCTCCA 
ACACACAACC ATAATTCTGT TCCATTCTCC AATTTTGGAC CCATTGGGAC TCCAGATAAC 
AGGGATAGAA GGACTGCAGA TCGGTGGAAA ACTGATAAGC CAGCCATGGG TGGGTTTGGC 
ATTGATTATC TCTCAGCAAC GTCATCCTCT GAGAGCAGTT GGCATCAGGC CAGCACTCCG 
AGTGGCACCT GGACAGGCCA TGGCCCTTCC ATGGAGGATT CCTCTGCTGT CCTCATGGAA 
AGCCTAAAGT CTATCTGGTC CAGTTCCATG ATGCATCCTG GACCTTCTGC TCTGGAGCAG 
CTGTTAATGC AGCAGAAGCA GAAACAGCAA CGGGGACAAG GCACCATGAA CCCTCCACAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001337148.1
CDS	312..3674
Translation	MRNWMFLQIVIVGENVSFKSQMRTENLKSEEHLKSSNIRQAEVLKADMTDSKLGPAEVWTSRQALQDLYQKMLVTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRANPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQTHTSAIVKPQSSSCSYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNLQKALSKALESRDEVKTKWGVSDFIKAFIKFHGHVYLSKSLEKLSPLREKLEEQFKRLLFQKAFNSQQLVHVTVINLFQLHHLRDFSNETEQHTYSQDEQLCWTQLLALFMSFLGILCKCPLQNESQEESYNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPHEEDLSSISATPLPEEFELQGFLALRPSFRNLDFSKGHQGITGDKEGQQRRIRQQRLISIGKWIADNQPRLIQCENEVGKLLFITEIPELILEDPSEAKENLILQETSVIESLAADGSPGLKSVLSTSRNLSNNCDTGEKPVVTFKENIKTREVNRDQGRSFPPKEVKSQTELRKTPVSEARKTPVTQTPTQASNSQFIPIHHPGAFPPLPSRPGFPPPTYVIPPPVAFSMGSGYTFPAGVSVPGTFLQPTAHSPAGNQVQAGKQSHIPYSQQRPSGPGPMNQGPQQSQPPSQQPLTSLPAQPTAQSTSQLQVQALTQQQQSPTKAVPALGKSPPHHSGFQQYQQADASKQLWNPPQVQGPLGKIMPVKQPYYLQTQDPIKLFEPSLQPPVMQQQPLEKKMKPFPMEPYNHNPSEVKVPEFYWDSSYSMADNRSVMAQQANIDRRGKRSPGVFRPEQDPVPRMPFEKSLLEKPSELMSHSSSFLSLTGFSLNQERYPNNSMFNEVYGKNLTSSSKAELSPSMAPQETSLYSLFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSVPFSNFGPIGTPDNRDRRTADRWKTDKPAMGGFGIDYLSATSSSESSWHQASTPSGTWTGHGPSMEDSSAVLMESLKSIWSSSMMHPGPSALEQLLMQQKQKQQRGQGTMNPPH

Target ORF information:

RefSeq Version	NM_001350219.2
Organism	Homo sapiens(human)
Definition	Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001350219.2

ORF Insert Sequence:

ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
CAGATGAGGA CCGAAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG 
GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCTGA AGTCTGGACA 
TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTAG TTACCGATTT GGAATACGCT 
TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCACGCCT TTAAGAATCA GATCACAACA 
CTGCAAGGCC AGGCAAAGAA TCGAGCAAAT CCGAATCGGA GTGAAGTTCA GGCAAACCTT 
TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA 
GTATTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AATTGGGAAT TATCAGCAAT 
AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC 
CAGCACTGCC TCGTCCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA 
GAGTCCTACT ATAGGCATGC AGCTCAGCTT GTCCCCTCCA ATGGTCAGCC TTATAATCAG 
TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACCA CAATTTTCTA CTACTGCAGA 
AGCATTGCTG TGAAGTTCCC TTTCCCAGCT GCCTCCACTA ATCTGCAAAA AGCACTTTCT 
AAAGCACTGG AAAGCCGAGA TGAGGTGAAA ACCAAGTGGG GTGTTTCTGA CTTCATCAAG 
GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT 
CTTCGAGAGA AATTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAAAAAGC TTTCAACTCT 
CAGCAGTTAG TTCATGTCAC TGTCATTAAC CTGTTTCAAC TTCATCACCT TCGTGACTTT 
AGCAATGAAA CCGAGCAGCA CACTTATAGC CAAGATGAGC AGCTATGTTG GACACAGTTG 
CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTACA GAATGAGTCT 
CAGGAGGAGT CCTACAATGC CTATCCTCTT CCAGCAGTCA AGGTCTCCAT GGACTGGCTA 
AGACTCAGAC CCAGGGTCTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCC 
TGGTTGATTT CTCTTCTGAA TAGTTTCCAT CCCCATGAAG AGGACCTCTC AAGTATTAGT 
GCGACACCAC TTCCAGAGGA GTTTGAATTA CAAGGATTTT TGGCATTGAG ACCTTCTTTC 
AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GGGACAAAGA AGGCCAGCAA 
CGACGAATAC GACAGCAACG CTTGATCTCT ATAGGCAAAT GGATTGCTGA TAATCAGCCA 
AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAA 
TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA AACATCTGTG 
ATAGAGTCGC TGGCTGCAGA TGGGAGCCCA GGGCTAAAAT CAGTGCTATC TACAAGCCGA 
AATTTAAGCA ACAACTGTGA CACAGGAGAG AAGCCAGTGG TTACCTTCAA AGAAAACATT 
AAGACACGAG AAGTGAACAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTAAAATCC 
CAGACAGAAC TAAGAAAGAC TCCAGTGTCT GAAGCCAGAA AAACACCTGT AACTCAAACC 
CCAACTCAAG CAAGTAACTC CCAGTTCATC CCCATTCATC ACCCTGGAGC CTTCCCTCCT 
CTTCCCAGCA GGCCAGGGTT TCCGCCCCCA ACATATGTTA TCCCCCCGCC TGTGGCATTT 
TCTATGGGCT CAGGTTACAC CTTCCCAGCT GGTGTTTCTG TCCCAGGAAC CTTTCTTCAG 
CCTACAGCTC ACTCTCCAGC AGGAAACCAG GTGCAAGCTG GGAAACAGTC CCACATTCCT 
TACAGCCAGC AACGGCCCTC TGGACCAGGG CCAATGAACC AGGGACCTCA ACAATCACAG 
CCACCTTCCC AGCAACCCCT TACATCTTTA CCAGCTCAGC CAACAGCACA GTCTACAAGC 
CAGCTGCAGG TTCAAGCTCT AACTCAGCAA CAACAATCCC CTACAAAAGC TGTGCCGGCT 
TTGGGGAAAA GCCCGCCTCA CCACTCTGGA TTCCAGCAGT ATCAACAGGC AGATGCCTCC 
AAACAGCTGT GGAATCCCCC TCAGGTTCAA GGCCCATTAG GGAAAATTAT GCCTGTGAAA 
CAGCCCTACT ACCTTCAGAC CCAAGACCCC ATAAAACTGT TTGAGCCGTC ATTGCAACCT 
CCTGTAATGC AGCAGCAGCC TCTAGAAAAA AAAATGAAGC CTTTTCCCAT GGAGCCATAT 
AACCATAATC CCTCAGAAGT CAAGGTCCCA GAATTCTACT GGGATTCTTC CTACAGCATG 
GCTGATAACA GATCTGTAAT GGCACAGCAA GCAAACATAG ACCGCAGGGG CAAACGGTCA 
CCAGGAGTCT TCCGTCCAGA GCAGGATCCT GTACCCAGAA TGCCGTTTGA GAAATCCTTA 
TTGGAGAAGC CCTCAGAGCT CATGTCACAT TCATCCTCTT TCCTGTCCCT CACCGGATTC 
TCTCTCAATC AGGAAAGATA CCCAAATAAT AGTATGTTCA ATGAGGTATA TGGGAAAAAC 
CTGACATCCA GCTCCAAAGC AGAACTCAGT CCCTCAATGG CCCCCCAGGA AACATCTCTG 
TATTCCCTTT TTGAAGGGAC TCCGTGGTCT CCATCACTTC CTGCCAGTTC AGATCATTCA 
ACACCAGCCA GCCAGTCTCC TCATTCCTCT AACCCAAGCA GCCTACCCAG CTCTCCTCCA 
ACACACAACC ATAATTCTGT TCCATTCTCC AATTTTGGAC CCATTGGGAC TCCAGATAAC 
AGGGATAGAA GGACTGCAGA TCGGTGGAAA ACTGATAAGC CAGCCATGGG TGGGTTTGGC 
ATTGATTATC TCTCAGCAAC GTCATCCTCT GAGAGCAGTT GGCATCAGGC CAGCACTCCG 
AGTGGCACCT GGACAGGCCA TGGCCCTTCC ATGGAGGATT CCTCTGCTGT CCTCATGGAA 
AGCCTAAAGT CTATCTGGTC CAGTTCCATG ATGCATCCTG GACCTTCTGC TCTGGAGCAG 
CTGTTAATGC AGCAGAAGCA GAAACAGCAA CGGGGACAAG GCACCATGAA CCCTCCACAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG7 ( XM_011510205.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011508507.1 Homo sapiens SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X1, mRNA.

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CloneID	OHu34879
Clone ID Related Accession (Same CDS sequence)	NM_001350220.1 , XM_011510205.2 , NM_001350220.2 , XM_005245649.3 , XM_011510205.3 , XM_005245649.4
Accession Version	XM_011510205.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3651bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	protein SMG7 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011510205.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
CAGATGAGGA CCGAAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG 
GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCTGA AGTCTGGACA 
TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTAG TTACCGATTT GGAATACGCT 
TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCACGCCT TTAAGAATCA GATCACAACA 
CTGCAAGGCC AGGCAAAGAA TCGAGCAAAT CCGAATCGGA GTGAAGTTCA GGCAAACCTT 
TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA 
GTATTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AATTGGGAAT TATCAGCAAT 
AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC 
CAGCACTGCC TCGTCCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA 
GAGTCCTACT ATAGGCATGC AGCTCAGCTT GTCCCCTCCA ATGGTCAGCC TTATAATCAG 
TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACCA CAATTTTCTA CTACTGCAGA 
AGCATTGCTG TGAAGTTCCC TTTCCCAGCT GCCTCCACTA ATCTGCAAAA AGCACTTTCT 
AAAGCACTGG AAAGCCGAGA TGAGGTGAAA ACCAAGTGGG GTGTTTCTGA CTTCATCAAG 
GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT 
CTTCGAGAGA AATTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAAAAAGC TTTCAACTCT 
CAGCAGTTAG TTCATGTCAC TGTCATTAAC CTGTTTCAAC TTCATCACCT TCGTGACTTT 
AGCAATGAAA CCGAGCAGCA CACTTATAGC CAAGATGAGC AGCTATGTTG GACACAGTTG 
CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTACA GAATGAGTCT 
CAGGAGGAGT CCTACAATGC CTATCCTCTT CCAGCAGTCA AGGTCTCCAT GGACTGGCTA 
AGACTCAGAC CCAGGGTCTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCC 
TGGTTGATTT CTCTTCTGAA TAGTTTCCAT CCCCATGAAG AGGACCTCTC AAGTATTAGT 
GCGACACCAC TTCCAGAGGA GTTTGAATTA CAAGGATTTT TGGCATTGAG ACCTTCTTTC 
AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GGGACAAAGA AGGCCAGCAA 
CGACGAATAC GACAGCAACG CTTGATCTCT ATAGGCAAAT GGATTGCTGA TAATCAGCCA 
AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAA 
TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA AACATCTGTG 
ATAGAGTCGC TGGCTGCAGA TGGGAGCCCA GGGCTAAAAT CAGTGCTATC TACAAGCCGA 
AATTTAAGCA ACAACTGTGA CACAGGAGAG AAGCCAGTGG TTACCTTCAA AGAAAACATT 
AAGACACGAG AAGTGAACAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTGAGAAGG 
GACTATAGCA AAGGAATAAC TGTAACTAAG AATGATGGAA AGAAGGACAA CAACAAGAGG 
AAAACTGAAA CCAAGAAATG CACCTTAGAA AAGTTACAGG AAACAGGAAA GCAGAATGTG 
GCAGTGCAGG TAAAATCCCA GACAGAACTA AGAAAGACTC CAGTGTCTGA AGCCAGAAAA 
ACACCTGTAA CTCAAACCCC AACTCAAGCA AGTAACTCCC AGTTCATCCC CATTCATCAC 
CCTGGAGCCT TCCCTCCTCT TCCCAGCAGG CCAGGGTTTC CGCCCCCAAC ATATGTTATC 
CCCCCGCCTG TGGCATTTTC TATGGGCTCA GGTTACACCT TCCCAGCTGG TGTTTCTGTC 
CCAGGAACCT TTCTTCAGCC TACAGCTCAC TCTCCAGCAG GAAACCAGGT GCAAGCTGGG 
AAACAGTCCC ACATTCCTTA CAGCCAGCAA CGGCCCTCTG GACCAGGGCC AATGAACCAG 
GGACCTCAAC AATCACAGCC ACCTTCCCAG CAACCCCTTA CATCTTTACC AGCTCAGCCA 
ACAGCACAGT CTACAAGCCA GCTGCAGGTT CAAGCTCTAA CTCAGCAACA ACAATCCCCT 
ACAAAAGCTG TGCCGGCTTT GGGGAAAAGC CCGCCTCACC ACTCTGGATT CCAGCAGTAT 
CAACAGGCAG ATGCCTCCAA ACAGCTGTGG AATCCCCCTC AGGTTCAAGG CCCATTAGGG 
AAAATTATGC CTGTGAAACA GCCCTACTAC CTTCAGACCC AAGACCCCAT AAAACTGTTT 
GAGCCGTCAT TGCAACCTCC TGTAATGCAG CAGCAGCCTC TAGAAAAAAA AATGAAGCCT 
TTTCCCATGG AGCCATATAA CCATAATCCC TCAGAAGTCA AGGTCCCAGA ATTCTACTGG 
GATTCTTCCT ACAGCATGGC TGATAACAGA TCTGTAATGG CACAGCAAGC AAACATAGAC 
CGCAGGGGCA AACGGTCACC AGGAGTCTTC CGTCCAGAGC AGGATCCTGT ACCCAGAATG 
CCGTTTGAGG ACCCCAAGAG CTCCCCTCTG CTTCCTCCGG ACCTGTTAAA GAGTCTGGCT 
GCCTTGGAGG AAGAGGAAGA GCTGATTTTT TCTAACCCTC CTGATCTTTA CCCGGCTCTG 
CTGGGGCCTC TCGCCTCTCT TCCTGGACGA AGCCTTTTTA AATCCTTATT GGAGAAGCCC 
TCAGAGCTCA TGTCACATTC ATCCTCTTTC CTGTCCCTCA CCGGATTCTC TCTCAATCAG 
GAAAGATACC CAAATAATAG TATGTTCAAT GAGGTATATG GGAAAAACCT GACATCCAGC 
TCCAAAGCAG AACTCAGTCC CTCAATGGCC CCCCAGGAAA CATCTCTGTA TTCCCTTTTT 
GAAGGGACTC CGTGGTCTCC ATCACTTCCT GCCAGTTCAG ATCATTCAAC ACCAGCCAGC 
CAGTCTCCTC ATTCCTCTAA CCCAAGCAGC CTACCCAGCT CTCCTCCAAC ACACAACCAT 
AATTCTGTTC CATTCTCCAA TTTTGGACCC ATTGGGACTC CAGATAACAG GGATAGAAGG 
ACTGCAGATC GGTGGAAAAC TGATAAGCCA GCCATGGGTG GGTTTGGCAT TGATTATCTC 
TCAGCAACGT CATCCTCTGA GAGCAGTTGG CATCAGGCCA GCACTCCGAG TGGCACCTGG 
ACAGGCCATG GCCCTTCCAT GGAGGATTCC TCTGCTGTCC TCATGGAAAG CCTAAAGTCT 
ATCTGGTCCA GTTCCATGAT GCATCCTGGA CCTTCTGCTC TGGAGCAGCT GTTAATGCAG 
CAGAAGCAGA AACAGCAACG GGGACAAGGC ACCATGAACC CTCCACACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011508507.1
CDS	1027..4677
Translation	MRNWMFLQIVIVGENVSFKSQMRTENLKSEEHLKSSNIRQAEVLKADMTDSKLGPAEVWTSRQALQDLYQKMLVTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRANPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQTHTSAIVKPQSSSCSYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNLQKALSKALESRDEVKTKWGVSDFIKAFIKFHGHVYLSKSLEKLSPLREKLEEQFKRLLFQKAFNSQQLVHVTVINLFQLHHLRDFSNETEQHTYSQDEQLCWTQLLALFMSFLGILCKCPLQNESQEESYNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPHEEDLSSISATPLPEEFELQGFLALRPSFRNLDFSKGHQGITGDKEGQQRRIRQQRLISIGKWIADNQPRLIQCENEVGKLLFITEIPELILEDPSEAKENLILQETSVIESLAADGSPGLKSVLSTSRNLSNNCDTGEKPVVTFKENIKTREVNRDQGRSFPPKEVRRDYSKGITVTKNDGKKDNNKRKTETKKCTLEKLQETGKQNVAVQVKSQTELRKTPVSEARKTPVTQTPTQASNSQFIPIHHPGAFPPLPSRPGFPPPTYVIPPPVAFSMGSGYTFPAGVSVPGTFLQPTAHSPAGNQVQAGKQSHIPYSQQRPSGPGPMNQGPQQSQPPSQQPLTSLPAQPTAQSTSQLQVQALTQQQQSPTKAVPALGKSPPHHSGFQQYQQADASKQLWNPPQVQGPLGKIMPVKQPYYLQTQDPIKLFEPSLQPPVMQQQPLEKKMKPFPMEPYNHNPSEVKVPEFYWDSSYSMADNRSVMAQQANIDRRGKRSPGVFRPEQDPVPRMPFEDPKSSPLLPPDLLKSLAALEEEEELIFSNPPDLYPALLGPLASLPGRSLFKSLLEKPSELMSHSSSFLSLTGFSLNQERYPNNSMFNEVYGKNLTSSSKAELSPSMAPQETSLYSLFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSVPFSNFGPIGTPDNRDRRTADRWKTDKPAMGGFGIDYLSATSSSESSWHQASTPSGTWTGHGPSMEDSSAVLMESLKSIWSSSMMHPGPSALEQLLMQQKQKQQRGQGTMNPPH

Target ORF information:

RefSeq Version	XM_011510205.2
Organism	Homo sapiens(human)
Definition	Homo sapiens SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011510205.2

ORF Insert Sequence:

ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
CAGATGAGGA CCGAAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG 
GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCTGA AGTCTGGACA 
TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTAG TTACCGATTT GGAATACGCT 
TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCACGCCT TTAAGAATCA GATCACAACA 
CTGCAAGGCC AGGCAAAGAA TCGAGCAAAT CCGAATCGGA GTGAAGTTCA GGCAAACCTT 
TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA 
GTATTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AATTGGGAAT TATCAGCAAT 
AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC 
CAGCACTGCC TCGTCCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA 
GAGTCCTACT ATAGGCATGC AGCTCAGCTT GTCCCCTCCA ATGGTCAGCC TTATAATCAG 
TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACCA CAATTTTCTA CTACTGCAGA 
AGCATTGCTG TGAAGTTCCC TTTCCCAGCT GCCTCCACTA ATCTGCAAAA AGCACTTTCT 
AAAGCACTGG AAAGCCGAGA TGAGGTGAAA ACCAAGTGGG GTGTTTCTGA CTTCATCAAG 
GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT 
CTTCGAGAGA AATTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAAAAAGC TTTCAACTCT 
CAGCAGTTAG TTCATGTCAC TGTCATTAAC CTGTTTCAAC TTCATCACCT TCGTGACTTT 
AGCAATGAAA CCGAGCAGCA CACTTATAGC CAAGATGAGC AGCTATGTTG GACACAGTTG 
CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTACA GAATGAGTCT 
CAGGAGGAGT CCTACAATGC CTATCCTCTT CCAGCAGTCA AGGTCTCCAT GGACTGGCTA 
AGACTCAGAC CCAGGGTCTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCC 
TGGTTGATTT CTCTTCTGAA TAGTTTCCAT CCCCATGAAG AGGACCTCTC AAGTATTAGT 
GCGACACCAC TTCCAGAGGA GTTTGAATTA CAAGGATTTT TGGCATTGAG ACCTTCTTTC 
AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GGGACAAAGA AGGCCAGCAA 
CGACGAATAC GACAGCAACG CTTGATCTCT ATAGGCAAAT GGATTGCTGA TAATCAGCCA 
AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAA 
TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA AACATCTGTG 
ATAGAGTCGC TGGCTGCAGA TGGGAGCCCA GGGCTAAAAT CAGTGCTATC TACAAGCCGA 
AATTTAAGCA ACAACTGTGA CACAGGAGAG AAGCCAGTGG TTACCTTCAA AGAAAACATT 
AAGACACGAG AAGTGAACAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTGAGAAGG 
GACTATAGCA AAGGAATAAC TGTAACTAAG AATGATGGAA AGAAGGACAA CAACAAGAGG 
AAAACTGAAA CCAAGAAATG CACCTTAGAA AAGTTACAGG AAACAGGAAA GCAGAATGTG 
GCAGTGCAGG TAAAATCCCA GACAGAACTA AGAAAGACTC CAGTGTCTGA AGCCAGAAAA 
ACACCTGTAA CTCAAACCCC AACTCAAGCA AGTAACTCCC AGTTCATCCC CATTCATCAC 
CCTGGAGCCT TCCCTCCTCT TCCCAGCAGG CCAGGGTTTC CGCCCCCAAC ATATGTTATC 
CCCCCGCCTG TGGCATTTTC TATGGGCTCA GGTTACACCT TCCCAGCTGG TGTTTCTGTC 
CCAGGAACCT TTCTTCAGCC TACAGCTCAC TCTCCAGCAG GAAACCAGGT GCAAGCTGGG 
AAACAGTCCC ACATTCCTTA CAGCCAGCAA CGGCCCTCTG GACCAGGGCC AATGAACCAG 
GGACCTCAAC AATCACAGCC ACCTTCCCAG CAACCCCTTA CATCTTTACC AGCTCAGCCA 
ACAGCACAGT CTACAAGCCA GCTGCAGGTT CAAGCTCTAA CTCAGCAACA ACAATCCCCT 
ACAAAAGCTG TGCCGGCTTT GGGGAAAAGC CCGCCTCACC ACTCTGGATT CCAGCAGTAT 
CAACAGGCAG ATGCCTCCAA ACAGCTGTGG AATCCCCCTC AGGTTCAAGG CCCATTAGGG 
AAAATTATGC CTGTGAAACA GCCCTACTAC CTTCAGACCC AAGACCCCAT AAAACTGTTT 
GAGCCGTCAT TGCAACCTCC TGTAATGCAG CAGCAGCCTC TAGAAAAAAA AATGAAGCCT 
TTTCCCATGG AGCCATATAA CCATAATCCC TCAGAAGTCA AGGTCCCAGA ATTCTACTGG 
GATTCTTCCT ACAGCATGGC TGATAACAGA TCTGTAATGG CACAGCAAGC AAACATAGAC 
CGCAGGGGCA AACGGTCACC AGGAGTCTTC CGTCCAGAGC AGGATCCTGT ACCCAGAATG 
CCGTTTGAGG ACCCCAAGAG CTCCCCTCTG CTTCCTCCGG ACCTGTTAAA GAGTCTGGCT 
GCCTTGGAGG AAGAGGAAGA GCTGATTTTT TCTAACCCTC CTGATCTTTA CCCGGCTCTG 
CTGGGGCCTC TCGCCTCTCT TCCTGGACGA AGCCTTTTTA AATCCTTATT GGAGAAGCCC 
TCAGAGCTCA TGTCACATTC ATCCTCTTTC CTGTCCCTCA CCGGATTCTC TCTCAATCAG 
GAAAGATACC CAAATAATAG TATGTTCAAT GAGGTATATG GGAAAAACCT GACATCCAGC 
TCCAAAGCAG AACTCAGTCC CTCAATGGCC CCCCAGGAAA CATCTCTGTA TTCCCTTTTT 
GAAGGGACTC CGTGGTCTCC ATCACTTCCT GCCAGTTCAG ATCATTCAAC ACCAGCCAGC 
CAGTCTCCTC ATTCCTCTAA CCCAAGCAGC CTACCCAGCT CTCCTCCAAC ACACAACCAT 
AATTCTGTTC CATTCTCCAA TTTTGGACCC ATTGGGACTC CAGATAACAG GGATAGAAGG 
ACTGCAGATC GGTGGAAAAC TGATAAGCCA GCCATGGGTG GGTTTGGCAT TGATTATCTC 
TCAGCAACGT CATCCTCTGA GAGCAGTTGG CATCAGGCCA GCACTCCGAG TGGCACCTGG 
ACAGGCCATG GCCCTTCCAT GGAGGATTCC TCTGCTGTCC TCATGGAAAG CCTAAAGTCT 
ATCTGGTCCA GTTCCATGAT GCATCCTGGA CCTTCTGCTC TGGAGCAGCT GTTAATGCAG 
CAGAAGCAGA AACAGCAACG GGGACAAGGC ACCATGAACC CTCCACACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG7 ( XM_005245649.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005245706.1 Homo sapiens SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X3, mRNA.

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CloneID	OHu34879
Clone ID Related Accession (Same CDS sequence)	NM_001350220.1 , XM_011510205.2 , NM_001350220.2 , XM_005245649.3 , XM_011510205.3 , XM_005245649.4
Accession Version	XM_005245649.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3651bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	protein SMG7 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005245649.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
CAGATGAGGA CCGAAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG 
GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCTGA AGTCTGGACA 
TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTAG TTACCGATTT GGAATACGCT 
TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCACGCCT TTAAGAATCA GATCACAACA 
CTGCAAGGCC AGGCAAAGAA TCGAGCAAAT CCGAATCGGA GTGAAGTTCA GGCAAACCTT 
TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA 
GTATTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AATTGGGAAT TATCAGCAAT 
AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC 
CAGCACTGCC TCGTCCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA 
GAGTCCTACT ATAGGCATGC AGCTCAGCTT GTCCCCTCCA ATGGTCAGCC TTATAATCAG 
TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACCA CAATTTTCTA CTACTGCAGA 
AGCATTGCTG TGAAGTTCCC TTTCCCAGCT GCCTCCACTA ATCTGCAAAA AGCACTTTCT 
AAAGCACTGG AAAGCCGAGA TGAGGTGAAA ACCAAGTGGG GTGTTTCTGA CTTCATCAAG 
GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT 
CTTCGAGAGA AATTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAAAAAGC TTTCAACTCT 
CAGCAGTTAG TTCATGTCAC TGTCATTAAC CTGTTTCAAC TTCATCACCT TCGTGACTTT 
AGCAATGAAA CCGAGCAGCA CACTTATAGC CAAGATGAGC AGCTATGTTG GACACAGTTG 
CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTACA GAATGAGTCT 
CAGGAGGAGT CCTACAATGC CTATCCTCTT CCAGCAGTCA AGGTCTCCAT GGACTGGCTA 
AGACTCAGAC CCAGGGTCTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCC 
TGGTTGATTT CTCTTCTGAA TAGTTTCCAT CCCCATGAAG AGGACCTCTC AAGTATTAGT 
GCGACACCAC TTCCAGAGGA GTTTGAATTA CAAGGATTTT TGGCATTGAG ACCTTCTTTC 
AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GGGACAAAGA AGGCCAGCAA 
CGACGAATAC GACAGCAACG CTTGATCTCT ATAGGCAAAT GGATTGCTGA TAATCAGCCA 
AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAA 
TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA AACATCTGTG 
ATAGAGTCGC TGGCTGCAGA TGGGAGCCCA GGGCTAAAAT CAGTGCTATC TACAAGCCGA 
AATTTAAGCA ACAACTGTGA CACAGGAGAG AAGCCAGTGG TTACCTTCAA AGAAAACATT 
AAGACACGAG AAGTGAACAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTGAGAAGG 
GACTATAGCA AAGGAATAAC TGTAACTAAG AATGATGGAA AGAAGGACAA CAACAAGAGG 
AAAACTGAAA CCAAGAAATG CACCTTAGAA AAGTTACAGG AAACAGGAAA GCAGAATGTG 
GCAGTGCAGG TAAAATCCCA GACAGAACTA AGAAAGACTC CAGTGTCTGA AGCCAGAAAA 
ACACCTGTAA CTCAAACCCC AACTCAAGCA AGTAACTCCC AGTTCATCCC CATTCATCAC 
CCTGGAGCCT TCCCTCCTCT TCCCAGCAGG CCAGGGTTTC CGCCCCCAAC ATATGTTATC 
CCCCCGCCTG TGGCATTTTC TATGGGCTCA GGTTACACCT TCCCAGCTGG TGTTTCTGTC 
CCAGGAACCT TTCTTCAGCC TACAGCTCAC TCTCCAGCAG GAAACCAGGT GCAAGCTGGG 
AAACAGTCCC ACATTCCTTA CAGCCAGCAA CGGCCCTCTG GACCAGGGCC AATGAACCAG 
GGACCTCAAC AATCACAGCC ACCTTCCCAG CAACCCCTTA CATCTTTACC AGCTCAGCCA 
ACAGCACAGT CTACAAGCCA GCTGCAGGTT CAAGCTCTAA CTCAGCAACA ACAATCCCCT 
ACAAAAGCTG TGCCGGCTTT GGGGAAAAGC CCGCCTCACC ACTCTGGATT CCAGCAGTAT 
CAACAGGCAG ATGCCTCCAA ACAGCTGTGG AATCCCCCTC AGGTTCAAGG CCCATTAGGG 
AAAATTATGC CTGTGAAACA GCCCTACTAC CTTCAGACCC AAGACCCCAT AAAACTGTTT 
GAGCCGTCAT TGCAACCTCC TGTAATGCAG CAGCAGCCTC TAGAAAAAAA AATGAAGCCT 
TTTCCCATGG AGCCATATAA CCATAATCCC TCAGAAGTCA AGGTCCCAGA ATTCTACTGG 
GATTCTTCCT ACAGCATGGC TGATAACAGA TCTGTAATGG CACAGCAAGC AAACATAGAC 
CGCAGGGGCA AACGGTCACC AGGAGTCTTC CGTCCAGAGC AGGATCCTGT ACCCAGAATG 
CCGTTTGAGG ACCCCAAGAG CTCCCCTCTG CTTCCTCCGG ACCTGTTAAA GAGTCTGGCT 
GCCTTGGAGG AAGAGGAAGA GCTGATTTTT TCTAACCCTC CTGATCTTTA CCCGGCTCTG 
CTGGGGCCTC TCGCCTCTCT TCCTGGACGA AGCCTTTTTA AATCCTTATT GGAGAAGCCC 
TCAGAGCTCA TGTCACATTC ATCCTCTTTC CTGTCCCTCA CCGGATTCTC TCTCAATCAG 
GAAAGATACC CAAATAATAG TATGTTCAAT GAGGTATATG GGAAAAACCT GACATCCAGC 
TCCAAAGCAG AACTCAGTCC CTCAATGGCC CCCCAGGAAA CATCTCTGTA TTCCCTTTTT 
GAAGGGACTC CGTGGTCTCC ATCACTTCCT GCCAGTTCAG ATCATTCAAC ACCAGCCAGC 
CAGTCTCCTC ATTCCTCTAA CCCAAGCAGC CTACCCAGCT CTCCTCCAAC ACACAACCAT 
AATTCTGTTC CATTCTCCAA TTTTGGACCC ATTGGGACTC CAGATAACAG GGATAGAAGG 
ACTGCAGATC GGTGGAAAAC TGATAAGCCA GCCATGGGTG GGTTTGGCAT TGATTATCTC 
TCAGCAACGT CATCCTCTGA GAGCAGTTGG CATCAGGCCA GCACTCCGAG TGGCACCTGG 
ACAGGCCATG GCCCTTCCAT GGAGGATTCC TCTGCTGTCC TCATGGAAAG CCTAAAGTCT 
ATCTGGTCCA GTTCCATGAT GCATCCTGGA CCTTCTGCTC TGGAGCAGCT GTTAATGCAG 
CAGAAGCAGA AACAGCAACG GGGACAAGGC ACCATGAACC CTCCACACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005245706.1
CDS	712..4362
Translation	MRNWMFLQIVIVGENVSFKSQMRTENLKSEEHLKSSNIRQAEVLKADMTDSKLGPAEVWTSRQALQDLYQKMLVTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRANPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQTHTSAIVKPQSSSCSYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNLQKALSKALESRDEVKTKWGVSDFIKAFIKFHGHVYLSKSLEKLSPLREKLEEQFKRLLFQKAFNSQQLVHVTVINLFQLHHLRDFSNETEQHTYSQDEQLCWTQLLALFMSFLGILCKCPLQNESQEESYNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPHEEDLSSISATPLPEEFELQGFLALRPSFRNLDFSKGHQGITGDKEGQQRRIRQQRLISIGKWIADNQPRLIQCENEVGKLLFITEIPELILEDPSEAKENLILQETSVIESLAADGSPGLKSVLSTSRNLSNNCDTGEKPVVTFKENIKTREVNRDQGRSFPPKEVRRDYSKGITVTKNDGKKDNNKRKTETKKCTLEKLQETGKQNVAVQVKSQTELRKTPVSEARKTPVTQTPTQASNSQFIPIHHPGAFPPLPSRPGFPPPTYVIPPPVAFSMGSGYTFPAGVSVPGTFLQPTAHSPAGNQVQAGKQSHIPYSQQRPSGPGPMNQGPQQSQPPSQQPLTSLPAQPTAQSTSQLQVQALTQQQQSPTKAVPALGKSPPHHSGFQQYQQADASKQLWNPPQVQGPLGKIMPVKQPYYLQTQDPIKLFEPSLQPPVMQQQPLEKKMKPFPMEPYNHNPSEVKVPEFYWDSSYSMADNRSVMAQQANIDRRGKRSPGVFRPEQDPVPRMPFEDPKSSPLLPPDLLKSLAALEEEEELIFSNPPDLYPALLGPLASLPGRSLFKSLLEKPSELMSHSSSFLSLTGFSLNQERYPNNSMFNEVYGKNLTSSSKAELSPSMAPQETSLYSLFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSVPFSNFGPIGTPDNRDRRTADRWKTDKPAMGGFGIDYLSATSSSESSWHQASTPSGTWTGHGPSMEDSSAVLMESLKSIWSSSMMHPGPSALEQLLMQQKQKQQRGQGTMNPPH

Target ORF information:

RefSeq Version	XM_005245649.3
Organism	Homo sapiens(human)
Definition	Homo sapiens SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005245649.3

ORF Insert Sequence:

ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
CAGATGAGGA CCGAAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG 
GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCTGA AGTCTGGACA 
TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTAG TTACCGATTT GGAATACGCT 
TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCACGCCT TTAAGAATCA GATCACAACA 
CTGCAAGGCC AGGCAAAGAA TCGAGCAAAT CCGAATCGGA GTGAAGTTCA GGCAAACCTT 
TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA 
GTATTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AATTGGGAAT TATCAGCAAT 
AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC 
CAGCACTGCC TCGTCCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA 
GAGTCCTACT ATAGGCATGC AGCTCAGCTT GTCCCCTCCA ATGGTCAGCC TTATAATCAG 
TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACCA CAATTTTCTA CTACTGCAGA 
AGCATTGCTG TGAAGTTCCC TTTCCCAGCT GCCTCCACTA ATCTGCAAAA AGCACTTTCT 
AAAGCACTGG AAAGCCGAGA TGAGGTGAAA ACCAAGTGGG GTGTTTCTGA CTTCATCAAG 
GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT 
CTTCGAGAGA AATTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAAAAAGC TTTCAACTCT 
CAGCAGTTAG TTCATGTCAC TGTCATTAAC CTGTTTCAAC TTCATCACCT TCGTGACTTT 
AGCAATGAAA CCGAGCAGCA CACTTATAGC CAAGATGAGC AGCTATGTTG GACACAGTTG 
CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTACA GAATGAGTCT 
CAGGAGGAGT CCTACAATGC CTATCCTCTT CCAGCAGTCA AGGTCTCCAT GGACTGGCTA 
AGACTCAGAC CCAGGGTCTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCC 
TGGTTGATTT CTCTTCTGAA TAGTTTCCAT CCCCATGAAG AGGACCTCTC AAGTATTAGT 
GCGACACCAC TTCCAGAGGA GTTTGAATTA CAAGGATTTT TGGCATTGAG ACCTTCTTTC 
AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GGGACAAAGA AGGCCAGCAA 
CGACGAATAC GACAGCAACG CTTGATCTCT ATAGGCAAAT GGATTGCTGA TAATCAGCCA 
AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAA 
TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA AACATCTGTG 
ATAGAGTCGC TGGCTGCAGA TGGGAGCCCA GGGCTAAAAT CAGTGCTATC TACAAGCCGA 
AATTTAAGCA ACAACTGTGA CACAGGAGAG AAGCCAGTGG TTACCTTCAA AGAAAACATT 
AAGACACGAG AAGTGAACAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTGAGAAGG 
GACTATAGCA AAGGAATAAC TGTAACTAAG AATGATGGAA AGAAGGACAA CAACAAGAGG 
AAAACTGAAA CCAAGAAATG CACCTTAGAA AAGTTACAGG AAACAGGAAA GCAGAATGTG 
GCAGTGCAGG TAAAATCCCA GACAGAACTA AGAAAGACTC CAGTGTCTGA AGCCAGAAAA 
ACACCTGTAA CTCAAACCCC AACTCAAGCA AGTAACTCCC AGTTCATCCC CATTCATCAC 
CCTGGAGCCT TCCCTCCTCT TCCCAGCAGG CCAGGGTTTC CGCCCCCAAC ATATGTTATC 
CCCCCGCCTG TGGCATTTTC TATGGGCTCA GGTTACACCT TCCCAGCTGG TGTTTCTGTC 
CCAGGAACCT TTCTTCAGCC TACAGCTCAC TCTCCAGCAG GAAACCAGGT GCAAGCTGGG 
AAACAGTCCC ACATTCCTTA CAGCCAGCAA CGGCCCTCTG GACCAGGGCC AATGAACCAG 
GGACCTCAAC AATCACAGCC ACCTTCCCAG CAACCCCTTA CATCTTTACC AGCTCAGCCA 
ACAGCACAGT CTACAAGCCA GCTGCAGGTT CAAGCTCTAA CTCAGCAACA ACAATCCCCT 
ACAAAAGCTG TGCCGGCTTT GGGGAAAAGC CCGCCTCACC ACTCTGGATT CCAGCAGTAT 
CAACAGGCAG ATGCCTCCAA ACAGCTGTGG AATCCCCCTC AGGTTCAAGG CCCATTAGGG 
AAAATTATGC CTGTGAAACA GCCCTACTAC CTTCAGACCC AAGACCCCAT AAAACTGTTT 
GAGCCGTCAT TGCAACCTCC TGTAATGCAG CAGCAGCCTC TAGAAAAAAA AATGAAGCCT 
TTTCCCATGG AGCCATATAA CCATAATCCC TCAGAAGTCA AGGTCCCAGA ATTCTACTGG 
GATTCTTCCT ACAGCATGGC TGATAACAGA TCTGTAATGG CACAGCAAGC AAACATAGAC 
CGCAGGGGCA AACGGTCACC AGGAGTCTTC CGTCCAGAGC AGGATCCTGT ACCCAGAATG 
CCGTTTGAGG ACCCCAAGAG CTCCCCTCTG CTTCCTCCGG ACCTGTTAAA GAGTCTGGCT 
GCCTTGGAGG AAGAGGAAGA GCTGATTTTT TCTAACCCTC CTGATCTTTA CCCGGCTCTG 
CTGGGGCCTC TCGCCTCTCT TCCTGGACGA AGCCTTTTTA AATCCTTATT GGAGAAGCCC 
TCAGAGCTCA TGTCACATTC ATCCTCTTTC CTGTCCCTCA CCGGATTCTC TCTCAATCAG 
GAAAGATACC CAAATAATAG TATGTTCAAT GAGGTATATG GGAAAAACCT GACATCCAGC 
TCCAAAGCAG AACTCAGTCC CTCAATGGCC CCCCAGGAAA CATCTCTGTA TTCCCTTTTT 
GAAGGGACTC CGTGGTCTCC ATCACTTCCT GCCAGTTCAG ATCATTCAAC ACCAGCCAGC 
CAGTCTCCTC ATTCCTCTAA CCCAAGCAGC CTACCCAGCT CTCCTCCAAC ACACAACCAT 
AATTCTGTTC CATTCTCCAA TTTTGGACCC ATTGGGACTC CAGATAACAG GGATAGAAGG 
ACTGCAGATC GGTGGAAAAC TGATAAGCCA GCCATGGGTG GGTTTGGCAT TGATTATCTC 
TCAGCAACGT CATCCTCTGA GAGCAGTTGG CATCAGGCCA GCACTCCGAG TGGCACCTGG 
ACAGGCCATG GCCCTTCCAT GGAGGATTCC TCTGCTGTCC TCATGGAAAG CCTAAAGTCT 
ATCTGGTCCA GTTCCATGAT GCATCCTGGA CCTTCTGCTC TGGAGCAGCT GTTAATGCAG 
CAGAAGCAGA AACAGCAACG GGGACAAGGC ACCATGAACC CTCCACACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG7 ( XM_011510205.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011508507.1 Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu34879
Clone ID Related Accession (Same CDS sequence)	NM_001350220.1 , XM_011510205.2 , NM_001350220.2 , XM_005245649.3 , XM_011510205.3 , XM_005245649.4
Accession Version	XM_011510205.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3651bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein SMG7 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011510205.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
CAGATGAGGA CCGAAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG 
GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCTGA AGTCTGGACA 
TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTAG TTACCGATTT GGAATACGCT 
TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCACGCCT TTAAGAATCA GATCACAACA 
CTGCAAGGCC AGGCAAAGAA TCGAGCAAAT CCGAATCGGA GTGAAGTTCA GGCAAACCTT 
TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA 
GTATTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AATTGGGAAT TATCAGCAAT 
AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC 
CAGCACTGCC TCGTCCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA 
GAGTCCTACT ATAGGCATGC AGCTCAGCTT GTCCCCTCCA ATGGTCAGCC TTATAATCAG 
TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACCA CAATTTTCTA CTACTGCAGA 
AGCATTGCTG TGAAGTTCCC TTTCCCAGCT GCCTCCACTA ATCTGCAAAA AGCACTTTCT 
AAAGCACTGG AAAGCCGAGA TGAGGTGAAA ACCAAGTGGG GTGTTTCTGA CTTCATCAAG 
GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT 
CTTCGAGAGA AATTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAAAAAGC TTTCAACTCT 
CAGCAGTTAG TTCATGTCAC TGTCATTAAC CTGTTTCAAC TTCATCACCT TCGTGACTTT 
AGCAATGAAA CCGAGCAGCA CACTTATAGC CAAGATGAGC AGCTATGTTG GACACAGTTG 
CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTACA GAATGAGTCT 
CAGGAGGAGT CCTACAATGC CTATCCTCTT CCAGCAGTCA AGGTCTCCAT GGACTGGCTA 
AGACTCAGAC CCAGGGTCTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCC 
TGGTTGATTT CTCTTCTGAA TAGTTTCCAT CCCCATGAAG AGGACCTCTC AAGTATTAGT 
GCGACACCAC TTCCAGAGGA GTTTGAATTA CAAGGATTTT TGGCATTGAG ACCTTCTTTC 
AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GGGACAAAGA AGGCCAGCAA 
CGACGAATAC GACAGCAACG CTTGATCTCT ATAGGCAAAT GGATTGCTGA TAATCAGCCA 
AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAA 
TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA AACATCTGTG 
ATAGAGTCGC TGGCTGCAGA TGGGAGCCCA GGGCTAAAAT CAGTGCTATC TACAAGCCGA 
AATTTAAGCA ACAACTGTGA CACAGGAGAG AAGCCAGTGG TTACCTTCAA AGAAAACATT 
AAGACACGAG AAGTGAACAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTGAGAAGG 
GACTATAGCA AAGGAATAAC TGTAACTAAG AATGATGGAA AGAAGGACAA CAACAAGAGG 
AAAACTGAAA CCAAGAAATG CACCTTAGAA AAGTTACAGG AAACAGGAAA GCAGAATGTG 
GCAGTGCAGG TAAAATCCCA GACAGAACTA AGAAAGACTC CAGTGTCTGA AGCCAGAAAA 
ACACCTGTAA CTCAAACCCC AACTCAAGCA AGTAACTCCC AGTTCATCCC CATTCATCAC 
CCTGGAGCCT TCCCTCCTCT TCCCAGCAGG CCAGGGTTTC CGCCCCCAAC ATATGTTATC 
CCCCCGCCTG TGGCATTTTC TATGGGCTCA GGTTACACCT TCCCAGCTGG TGTTTCTGTC 
CCAGGAACCT TTCTTCAGCC TACAGCTCAC TCTCCAGCAG GAAACCAGGT GCAAGCTGGG 
AAACAGTCCC ACATTCCTTA CAGCCAGCAA CGGCCCTCTG GACCAGGGCC AATGAACCAG 
GGACCTCAAC AATCACAGCC ACCTTCCCAG CAACCCCTTA CATCTTTACC AGCTCAGCCA 
ACAGCACAGT CTACAAGCCA GCTGCAGGTT CAAGCTCTAA CTCAGCAACA ACAATCCCCT 
ACAAAAGCTG TGCCGGCTTT GGGGAAAAGC CCGCCTCACC ACTCTGGATT CCAGCAGTAT 
CAACAGGCAG ATGCCTCCAA ACAGCTGTGG AATCCCCCTC AGGTTCAAGG CCCATTAGGG 
AAAATTATGC CTGTGAAACA GCCCTACTAC CTTCAGACCC AAGACCCCAT AAAACTGTTT 
GAGCCGTCAT TGCAACCTCC TGTAATGCAG CAGCAGCCTC TAGAAAAAAA AATGAAGCCT 
TTTCCCATGG AGCCATATAA CCATAATCCC TCAGAAGTCA AGGTCCCAGA ATTCTACTGG 
GATTCTTCCT ACAGCATGGC TGATAACAGA TCTGTAATGG CACAGCAAGC AAACATAGAC 
CGCAGGGGCA AACGGTCACC AGGAGTCTTC CGTCCAGAGC AGGATCCTGT ACCCAGAATG 
CCGTTTGAGG ACCCCAAGAG CTCCCCTCTG CTTCCTCCGG ACCTGTTAAA GAGTCTGGCT 
GCCTTGGAGG AAGAGGAAGA GCTGATTTTT TCTAACCCTC CTGATCTTTA CCCGGCTCTG 
CTGGGGCCTC TCGCCTCTCT TCCTGGACGA AGCCTTTTTA AATCCTTATT GGAGAAGCCC 
TCAGAGCTCA TGTCACATTC ATCCTCTTTC CTGTCCCTCA CCGGATTCTC TCTCAATCAG 
GAAAGATACC CAAATAATAG TATGTTCAAT GAGGTATATG GGAAAAACCT GACATCCAGC 
TCCAAAGCAG AACTCAGTCC CTCAATGGCC CCCCAGGAAA CATCTCTGTA TTCCCTTTTT 
GAAGGGACTC CGTGGTCTCC ATCACTTCCT GCCAGTTCAG ATCATTCAAC ACCAGCCAGC 
CAGTCTCCTC ATTCCTCTAA CCCAAGCAGC CTACCCAGCT CTCCTCCAAC ACACAACCAT 
AATTCTGTTC CATTCTCCAA TTTTGGACCC ATTGGGACTC CAGATAACAG GGATAGAAGG 
ACTGCAGATC GGTGGAAAAC TGATAAGCCA GCCATGGGTG GGTTTGGCAT TGATTATCTC 
TCAGCAACGT CATCCTCTGA GAGCAGTTGG CATCAGGCCA GCACTCCGAG TGGCACCTGG 
ACAGGCCATG GCCCTTCCAT GGAGGATTCC TCTGCTGTCC TCATGGAAAG CCTAAAGTCT 
ATCTGGTCCA GTTCCATGAT GCATCCTGGA CCTTCTGCTC TGGAGCAGCT GTTAATGCAG 
CAGAAGCAGA AACAGCAACG GGGACAAGGC ACCATGAACC CTCCACACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011508507.1
CDS	996..4646
Translation	MRNWMFLQIVIVGENVSFKSQMRTENLKSEEHLKSSNIRQAEVLKADMTDSKLGPAEVWTSRQALQDLYQKMLVTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRANPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQTHTSAIVKPQSSSCSYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNLQKALSKALESRDEVKTKWGVSDFIKAFIKFHGHVYLSKSLEKLSPLREKLEEQFKRLLFQKAFNSQQLVHVTVINLFQLHHLRDFSNETEQHTYSQDEQLCWTQLLALFMSFLGILCKCPLQNESQEESYNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPHEEDLSSISATPLPEEFELQGFLALRPSFRNLDFSKGHQGITGDKEGQQRRIRQQRLISIGKWIADNQPRLIQCENEVGKLLFITEIPELILEDPSEAKENLILQETSVIESLAADGSPGLKSVLSTSRNLSNNCDTGEKPVVTFKENIKTREVNRDQGRSFPPKEVRRDYSKGITVTKNDGKKDNNKRKTETKKCTLEKLQETGKQNVAVQVKSQTELRKTPVSEARKTPVTQTPTQASNSQFIPIHHPGAFPPLPSRPGFPPPTYVIPPPVAFSMGSGYTFPAGVSVPGTFLQPTAHSPAGNQVQAGKQSHIPYSQQRPSGPGPMNQGPQQSQPPSQQPLTSLPAQPTAQSTSQLQVQALTQQQQSPTKAVPALGKSPPHHSGFQQYQQADASKQLWNPPQVQGPLGKIMPVKQPYYLQTQDPIKLFEPSLQPPVMQQQPLEKKMKPFPMEPYNHNPSEVKVPEFYWDSSYSMADNRSVMAQQANIDRRGKRSPGVFRPEQDPVPRMPFEDPKSSPLLPPDLLKSLAALEEEEELIFSNPPDLYPALLGPLASLPGRSLFKSLLEKPSELMSHSSSFLSLTGFSLNQERYPNNSMFNEVYGKNLTSSSKAELSPSMAPQETSLYSLFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSVPFSNFGPIGTPDNRDRRTADRWKTDKPAMGGFGIDYLSATSSSESSWHQASTPSGTWTGHGPSMEDSSAVLMESLKSIWSSSMMHPGPSALEQLLMQQKQKQQRGQGTMNPPH

Target ORF information:

RefSeq Version	XM_011510205.3
Organism	Homo sapiens(human)
Definition	Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011510205.3

ORF Insert Sequence:

ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
CAGATGAGGA CCGAAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG 
GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCTGA AGTCTGGACA 
TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTAG TTACCGATTT GGAATACGCT 
TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCACGCCT TTAAGAATCA GATCACAACA 
CTGCAAGGCC AGGCAAAGAA TCGAGCAAAT CCGAATCGGA GTGAAGTTCA GGCAAACCTT 
TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA 
GTATTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AATTGGGAAT TATCAGCAAT 
AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC 
CAGCACTGCC TCGTCCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA 
GAGTCCTACT ATAGGCATGC AGCTCAGCTT GTCCCCTCCA ATGGTCAGCC TTATAATCAG 
TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACCA CAATTTTCTA CTACTGCAGA 
AGCATTGCTG TGAAGTTCCC TTTCCCAGCT GCCTCCACTA ATCTGCAAAA AGCACTTTCT 
AAAGCACTGG AAAGCCGAGA TGAGGTGAAA ACCAAGTGGG GTGTTTCTGA CTTCATCAAG 
GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT 
CTTCGAGAGA AATTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAAAAAGC TTTCAACTCT 
CAGCAGTTAG TTCATGTCAC TGTCATTAAC CTGTTTCAAC TTCATCACCT TCGTGACTTT 
AGCAATGAAA CCGAGCAGCA CACTTATAGC CAAGATGAGC AGCTATGTTG GACACAGTTG 
CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTACA GAATGAGTCT 
CAGGAGGAGT CCTACAATGC CTATCCTCTT CCAGCAGTCA AGGTCTCCAT GGACTGGCTA 
AGACTCAGAC CCAGGGTCTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCC 
TGGTTGATTT CTCTTCTGAA TAGTTTCCAT CCCCATGAAG AGGACCTCTC AAGTATTAGT 
GCGACACCAC TTCCAGAGGA GTTTGAATTA CAAGGATTTT TGGCATTGAG ACCTTCTTTC 
AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GGGACAAAGA AGGCCAGCAA 
CGACGAATAC GACAGCAACG CTTGATCTCT ATAGGCAAAT GGATTGCTGA TAATCAGCCA 
AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAA 
TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA AACATCTGTG 
ATAGAGTCGC TGGCTGCAGA TGGGAGCCCA GGGCTAAAAT CAGTGCTATC TACAAGCCGA 
AATTTAAGCA ACAACTGTGA CACAGGAGAG AAGCCAGTGG TTACCTTCAA AGAAAACATT 
AAGACACGAG AAGTGAACAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTGAGAAGG 
GACTATAGCA AAGGAATAAC TGTAACTAAG AATGATGGAA AGAAGGACAA CAACAAGAGG 
AAAACTGAAA CCAAGAAATG CACCTTAGAA AAGTTACAGG AAACAGGAAA GCAGAATGTG 
GCAGTGCAGG TAAAATCCCA GACAGAACTA AGAAAGACTC CAGTGTCTGA AGCCAGAAAA 
ACACCTGTAA CTCAAACCCC AACTCAAGCA AGTAACTCCC AGTTCATCCC CATTCATCAC 
CCTGGAGCCT TCCCTCCTCT TCCCAGCAGG CCAGGGTTTC CGCCCCCAAC ATATGTTATC 
CCCCCGCCTG TGGCATTTTC TATGGGCTCA GGTTACACCT TCCCAGCTGG TGTTTCTGTC 
CCAGGAACCT TTCTTCAGCC TACAGCTCAC TCTCCAGCAG GAAACCAGGT GCAAGCTGGG 
AAACAGTCCC ACATTCCTTA CAGCCAGCAA CGGCCCTCTG GACCAGGGCC AATGAACCAG 
GGACCTCAAC AATCACAGCC ACCTTCCCAG CAACCCCTTA CATCTTTACC AGCTCAGCCA 
ACAGCACAGT CTACAAGCCA GCTGCAGGTT CAAGCTCTAA CTCAGCAACA ACAATCCCCT 
ACAAAAGCTG TGCCGGCTTT GGGGAAAAGC CCGCCTCACC ACTCTGGATT CCAGCAGTAT 
CAACAGGCAG ATGCCTCCAA ACAGCTGTGG AATCCCCCTC AGGTTCAAGG CCCATTAGGG 
AAAATTATGC CTGTGAAACA GCCCTACTAC CTTCAGACCC AAGACCCCAT AAAACTGTTT 
GAGCCGTCAT TGCAACCTCC TGTAATGCAG CAGCAGCCTC TAGAAAAAAA AATGAAGCCT 
TTTCCCATGG AGCCATATAA CCATAATCCC TCAGAAGTCA AGGTCCCAGA ATTCTACTGG 
GATTCTTCCT ACAGCATGGC TGATAACAGA TCTGTAATGG CACAGCAAGC AAACATAGAC 
CGCAGGGGCA AACGGTCACC AGGAGTCTTC CGTCCAGAGC AGGATCCTGT ACCCAGAATG 
CCGTTTGAGG ACCCCAAGAG CTCCCCTCTG CTTCCTCCGG ACCTGTTAAA GAGTCTGGCT 
GCCTTGGAGG AAGAGGAAGA GCTGATTTTT TCTAACCCTC CTGATCTTTA CCCGGCTCTG 
CTGGGGCCTC TCGCCTCTCT TCCTGGACGA AGCCTTTTTA AATCCTTATT GGAGAAGCCC 
TCAGAGCTCA TGTCACATTC ATCCTCTTTC CTGTCCCTCA CCGGATTCTC TCTCAATCAG 
GAAAGATACC CAAATAATAG TATGTTCAAT GAGGTATATG GGAAAAACCT GACATCCAGC 
TCCAAAGCAG AACTCAGTCC CTCAATGGCC CCCCAGGAAA CATCTCTGTA TTCCCTTTTT 
GAAGGGACTC CGTGGTCTCC ATCACTTCCT GCCAGTTCAG ATCATTCAAC ACCAGCCAGC 
CAGTCTCCTC ATTCCTCTAA CCCAAGCAGC CTACCCAGCT CTCCTCCAAC ACACAACCAT 
AATTCTGTTC CATTCTCCAA TTTTGGACCC ATTGGGACTC CAGATAACAG GGATAGAAGG 
ACTGCAGATC GGTGGAAAAC TGATAAGCCA GCCATGGGTG GGTTTGGCAT TGATTATCTC 
TCAGCAACGT CATCCTCTGA GAGCAGTTGG CATCAGGCCA GCACTCCGAG TGGCACCTGG 
ACAGGCCATG GCCCTTCCAT GGAGGATTCC TCTGCTGTCC TCATGGAAAG CCTAAAGTCT 
ATCTGGTCCA GTTCCATGAT GCATCCTGGA CCTTCTGCTC TGGAGCAGCT GTTAATGCAG 
CAGAAGCAGA AACAGCAACG GGGACAAGGC ACCATGAACC CTCCACACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG7 ( XM_005245649.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005245706.1 Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu34879
Clone ID Related Accession (Same CDS sequence)	NM_001350220.1 , XM_011510205.2 , NM_001350220.2 , XM_005245649.3 , XM_011510205.3 , XM_005245649.4
Accession Version	XM_005245649.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3651bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein SMG7 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005245649.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
CAGATGAGGA CCGAAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG 
GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCTGA AGTCTGGACA 
TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTAG TTACCGATTT GGAATACGCT 
TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCACGCCT TTAAGAATCA GATCACAACA 
CTGCAAGGCC AGGCAAAGAA TCGAGCAAAT CCGAATCGGA GTGAAGTTCA GGCAAACCTT 
TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA 
GTATTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AATTGGGAAT TATCAGCAAT 
AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC 
CAGCACTGCC TCGTCCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA 
GAGTCCTACT ATAGGCATGC AGCTCAGCTT GTCCCCTCCA ATGGTCAGCC TTATAATCAG 
TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACCA CAATTTTCTA CTACTGCAGA 
AGCATTGCTG TGAAGTTCCC TTTCCCAGCT GCCTCCACTA ATCTGCAAAA AGCACTTTCT 
AAAGCACTGG AAAGCCGAGA TGAGGTGAAA ACCAAGTGGG GTGTTTCTGA CTTCATCAAG 
GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT 
CTTCGAGAGA AATTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAAAAAGC TTTCAACTCT 
CAGCAGTTAG TTCATGTCAC TGTCATTAAC CTGTTTCAAC TTCATCACCT TCGTGACTTT 
AGCAATGAAA CCGAGCAGCA CACTTATAGC CAAGATGAGC AGCTATGTTG GACACAGTTG 
CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTACA GAATGAGTCT 
CAGGAGGAGT CCTACAATGC CTATCCTCTT CCAGCAGTCA AGGTCTCCAT GGACTGGCTA 
AGACTCAGAC CCAGGGTCTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCC 
TGGTTGATTT CTCTTCTGAA TAGTTTCCAT CCCCATGAAG AGGACCTCTC AAGTATTAGT 
GCGACACCAC TTCCAGAGGA GTTTGAATTA CAAGGATTTT TGGCATTGAG ACCTTCTTTC 
AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GGGACAAAGA AGGCCAGCAA 
CGACGAATAC GACAGCAACG CTTGATCTCT ATAGGCAAAT GGATTGCTGA TAATCAGCCA 
AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAA 
TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA AACATCTGTG 
ATAGAGTCGC TGGCTGCAGA TGGGAGCCCA GGGCTAAAAT CAGTGCTATC TACAAGCCGA 
AATTTAAGCA ACAACTGTGA CACAGGAGAG AAGCCAGTGG TTACCTTCAA AGAAAACATT 
AAGACACGAG AAGTGAACAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTGAGAAGG 
GACTATAGCA AAGGAATAAC TGTAACTAAG AATGATGGAA AGAAGGACAA CAACAAGAGG 
AAAACTGAAA CCAAGAAATG CACCTTAGAA AAGTTACAGG AAACAGGAAA GCAGAATGTG 
GCAGTGCAGG TAAAATCCCA GACAGAACTA AGAAAGACTC CAGTGTCTGA AGCCAGAAAA 
ACACCTGTAA CTCAAACCCC AACTCAAGCA AGTAACTCCC AGTTCATCCC CATTCATCAC 
CCTGGAGCCT TCCCTCCTCT TCCCAGCAGG CCAGGGTTTC CGCCCCCAAC ATATGTTATC 
CCCCCGCCTG TGGCATTTTC TATGGGCTCA GGTTACACCT TCCCAGCTGG TGTTTCTGTC 
CCAGGAACCT TTCTTCAGCC TACAGCTCAC TCTCCAGCAG GAAACCAGGT GCAAGCTGGG 
AAACAGTCCC ACATTCCTTA CAGCCAGCAA CGGCCCTCTG GACCAGGGCC AATGAACCAG 
GGACCTCAAC AATCACAGCC ACCTTCCCAG CAACCCCTTA CATCTTTACC AGCTCAGCCA 
ACAGCACAGT CTACAAGCCA GCTGCAGGTT CAAGCTCTAA CTCAGCAACA ACAATCCCCT 
ACAAAAGCTG TGCCGGCTTT GGGGAAAAGC CCGCCTCACC ACTCTGGATT CCAGCAGTAT 
CAACAGGCAG ATGCCTCCAA ACAGCTGTGG AATCCCCCTC AGGTTCAAGG CCCATTAGGG 
AAAATTATGC CTGTGAAACA GCCCTACTAC CTTCAGACCC AAGACCCCAT AAAACTGTTT 
GAGCCGTCAT TGCAACCTCC TGTAATGCAG CAGCAGCCTC TAGAAAAAAA AATGAAGCCT 
TTTCCCATGG AGCCATATAA CCATAATCCC TCAGAAGTCA AGGTCCCAGA ATTCTACTGG 
GATTCTTCCT ACAGCATGGC TGATAACAGA TCTGTAATGG CACAGCAAGC AAACATAGAC 
CGCAGGGGCA AACGGTCACC AGGAGTCTTC CGTCCAGAGC AGGATCCTGT ACCCAGAATG 
CCGTTTGAGG ACCCCAAGAG CTCCCCTCTG CTTCCTCCGG ACCTGTTAAA GAGTCTGGCT 
GCCTTGGAGG AAGAGGAAGA GCTGATTTTT TCTAACCCTC CTGATCTTTA CCCGGCTCTG 
CTGGGGCCTC TCGCCTCTCT TCCTGGACGA AGCCTTTTTA AATCCTTATT GGAGAAGCCC 
TCAGAGCTCA TGTCACATTC ATCCTCTTTC CTGTCCCTCA CCGGATTCTC TCTCAATCAG 
GAAAGATACC CAAATAATAG TATGTTCAAT GAGGTATATG GGAAAAACCT GACATCCAGC 
TCCAAAGCAG AACTCAGTCC CTCAATGGCC CCCCAGGAAA CATCTCTGTA TTCCCTTTTT 
GAAGGGACTC CGTGGTCTCC ATCACTTCCT GCCAGTTCAG ATCATTCAAC ACCAGCCAGC 
CAGTCTCCTC ATTCCTCTAA CCCAAGCAGC CTACCCAGCT CTCCTCCAAC ACACAACCAT 
AATTCTGTTC CATTCTCCAA TTTTGGACCC ATTGGGACTC CAGATAACAG GGATAGAAGG 
ACTGCAGATC GGTGGAAAAC TGATAAGCCA GCCATGGGTG GGTTTGGCAT TGATTATCTC 
TCAGCAACGT CATCCTCTGA GAGCAGTTGG CATCAGGCCA GCACTCCGAG TGGCACCTGG 
ACAGGCCATG GCCCTTCCAT GGAGGATTCC TCTGCTGTCC TCATGGAAAG CCTAAAGTCT 
ATCTGGTCCA GTTCCATGAT GCATCCTGGA CCTTCTGCTC TGGAGCAGCT GTTAATGCAG 
CAGAAGCAGA AACAGCAACG GGGACAAGGC ACCATGAACC CTCCACACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005245706.1
CDS	681..4331
Translation	MRNWMFLQIVIVGENVSFKSQMRTENLKSEEHLKSSNIRQAEVLKADMTDSKLGPAEVWTSRQALQDLYQKMLVTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRANPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQTHTSAIVKPQSSSCSYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNLQKALSKALESRDEVKTKWGVSDFIKAFIKFHGHVYLSKSLEKLSPLREKLEEQFKRLLFQKAFNSQQLVHVTVINLFQLHHLRDFSNETEQHTYSQDEQLCWTQLLALFMSFLGILCKCPLQNESQEESYNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPHEEDLSSISATPLPEEFELQGFLALRPSFRNLDFSKGHQGITGDKEGQQRRIRQQRLISIGKWIADNQPRLIQCENEVGKLLFITEIPELILEDPSEAKENLILQETSVIESLAADGSPGLKSVLSTSRNLSNNCDTGEKPVVTFKENIKTREVNRDQGRSFPPKEVRRDYSKGITVTKNDGKKDNNKRKTETKKCTLEKLQETGKQNVAVQVKSQTELRKTPVSEARKTPVTQTPTQASNSQFIPIHHPGAFPPLPSRPGFPPPTYVIPPPVAFSMGSGYTFPAGVSVPGTFLQPTAHSPAGNQVQAGKQSHIPYSQQRPSGPGPMNQGPQQSQPPSQQPLTSLPAQPTAQSTSQLQVQALTQQQQSPTKAVPALGKSPPHHSGFQQYQQADASKQLWNPPQVQGPLGKIMPVKQPYYLQTQDPIKLFEPSLQPPVMQQQPLEKKMKPFPMEPYNHNPSEVKVPEFYWDSSYSMADNRSVMAQQANIDRRGKRSPGVFRPEQDPVPRMPFEDPKSSPLLPPDLLKSLAALEEEEELIFSNPPDLYPALLGPLASLPGRSLFKSLLEKPSELMSHSSSFLSLTGFSLNQERYPNNSMFNEVYGKNLTSSSKAELSPSMAPQETSLYSLFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSVPFSNFGPIGTPDNRDRRTADRWKTDKPAMGGFGIDYLSATSSSESSWHQASTPSGTWTGHGPSMEDSSAVLMESLKSIWSSSMMHPGPSALEQLLMQQKQKQQRGQGTMNPPH

Target ORF information:

RefSeq Version	XM_005245649.4
Organism	Homo sapiens(human)
Definition	Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005245649.4

ORF Insert Sequence:

ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
CAGATGAGGA CCGAAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG 
GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCTGA AGTCTGGACA 
TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTAG TTACCGATTT GGAATACGCT 
TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCACGCCT TTAAGAATCA GATCACAACA 
CTGCAAGGCC AGGCAAAGAA TCGAGCAAAT CCGAATCGGA GTGAAGTTCA GGCAAACCTT 
TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA 
GTATTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AATTGGGAAT TATCAGCAAT 
AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC 
CAGCACTGCC TCGTCCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA 
GAGTCCTACT ATAGGCATGC AGCTCAGCTT GTCCCCTCCA ATGGTCAGCC TTATAATCAG 
TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACCA CAATTTTCTA CTACTGCAGA 
AGCATTGCTG TGAAGTTCCC TTTCCCAGCT GCCTCCACTA ATCTGCAAAA AGCACTTTCT 
AAAGCACTGG AAAGCCGAGA TGAGGTGAAA ACCAAGTGGG GTGTTTCTGA CTTCATCAAG 
GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT 
CTTCGAGAGA AATTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAAAAAGC TTTCAACTCT 
CAGCAGTTAG TTCATGTCAC TGTCATTAAC CTGTTTCAAC TTCATCACCT TCGTGACTTT 
AGCAATGAAA CCGAGCAGCA CACTTATAGC CAAGATGAGC AGCTATGTTG GACACAGTTG 
CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTACA GAATGAGTCT 
CAGGAGGAGT CCTACAATGC CTATCCTCTT CCAGCAGTCA AGGTCTCCAT GGACTGGCTA 
AGACTCAGAC CCAGGGTCTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCC 
TGGTTGATTT CTCTTCTGAA TAGTTTCCAT CCCCATGAAG AGGACCTCTC AAGTATTAGT 
GCGACACCAC TTCCAGAGGA GTTTGAATTA CAAGGATTTT TGGCATTGAG ACCTTCTTTC 
AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GGGACAAAGA AGGCCAGCAA 
CGACGAATAC GACAGCAACG CTTGATCTCT ATAGGCAAAT GGATTGCTGA TAATCAGCCA 
AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAA 
TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA AACATCTGTG 
ATAGAGTCGC TGGCTGCAGA TGGGAGCCCA GGGCTAAAAT CAGTGCTATC TACAAGCCGA 
AATTTAAGCA ACAACTGTGA CACAGGAGAG AAGCCAGTGG TTACCTTCAA AGAAAACATT 
AAGACACGAG AAGTGAACAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTGAGAAGG 
GACTATAGCA AAGGAATAAC TGTAACTAAG AATGATGGAA AGAAGGACAA CAACAAGAGG 
AAAACTGAAA CCAAGAAATG CACCTTAGAA AAGTTACAGG AAACAGGAAA GCAGAATGTG 
GCAGTGCAGG TAAAATCCCA GACAGAACTA AGAAAGACTC CAGTGTCTGA AGCCAGAAAA 
ACACCTGTAA CTCAAACCCC AACTCAAGCA AGTAACTCCC AGTTCATCCC CATTCATCAC 
CCTGGAGCCT TCCCTCCTCT TCCCAGCAGG CCAGGGTTTC CGCCCCCAAC ATATGTTATC 
CCCCCGCCTG TGGCATTTTC TATGGGCTCA GGTTACACCT TCCCAGCTGG TGTTTCTGTC 
CCAGGAACCT TTCTTCAGCC TACAGCTCAC TCTCCAGCAG GAAACCAGGT GCAAGCTGGG 
AAACAGTCCC ACATTCCTTA CAGCCAGCAA CGGCCCTCTG GACCAGGGCC AATGAACCAG 
GGACCTCAAC AATCACAGCC ACCTTCCCAG CAACCCCTTA CATCTTTACC AGCTCAGCCA 
ACAGCACAGT CTACAAGCCA GCTGCAGGTT CAAGCTCTAA CTCAGCAACA ACAATCCCCT 
ACAAAAGCTG TGCCGGCTTT GGGGAAAAGC CCGCCTCACC ACTCTGGATT CCAGCAGTAT 
CAACAGGCAG ATGCCTCCAA ACAGCTGTGG AATCCCCCTC AGGTTCAAGG CCCATTAGGG 
AAAATTATGC CTGTGAAACA GCCCTACTAC CTTCAGACCC AAGACCCCAT AAAACTGTTT 
GAGCCGTCAT TGCAACCTCC TGTAATGCAG CAGCAGCCTC TAGAAAAAAA AATGAAGCCT 
TTTCCCATGG AGCCATATAA CCATAATCCC TCAGAAGTCA AGGTCCCAGA ATTCTACTGG 
GATTCTTCCT ACAGCATGGC TGATAACAGA TCTGTAATGG CACAGCAAGC AAACATAGAC 
CGCAGGGGCA AACGGTCACC AGGAGTCTTC CGTCCAGAGC AGGATCCTGT ACCCAGAATG 
CCGTTTGAGG ACCCCAAGAG CTCCCCTCTG CTTCCTCCGG ACCTGTTAAA GAGTCTGGCT 
GCCTTGGAGG AAGAGGAAGA GCTGATTTTT TCTAACCCTC CTGATCTTTA CCCGGCTCTG 
CTGGGGCCTC TCGCCTCTCT TCCTGGACGA AGCCTTTTTA AATCCTTATT GGAGAAGCCC 
TCAGAGCTCA TGTCACATTC ATCCTCTTTC CTGTCCCTCA CCGGATTCTC TCTCAATCAG 
GAAAGATACC CAAATAATAG TATGTTCAAT GAGGTATATG GGAAAAACCT GACATCCAGC 
TCCAAAGCAG AACTCAGTCC CTCAATGGCC CCCCAGGAAA CATCTCTGTA TTCCCTTTTT 
GAAGGGACTC CGTGGTCTCC ATCACTTCCT GCCAGTTCAG ATCATTCAAC ACCAGCCAGC 
CAGTCTCCTC ATTCCTCTAA CCCAAGCAGC CTACCCAGCT CTCCTCCAAC ACACAACCAT 
AATTCTGTTC CATTCTCCAA TTTTGGACCC ATTGGGACTC CAGATAACAG GGATAGAAGG 
ACTGCAGATC GGTGGAAAAC TGATAAGCCA GCCATGGGTG GGTTTGGCAT TGATTATCTC 
TCAGCAACGT CATCCTCTGA GAGCAGTTGG CATCAGGCCA GCACTCCGAG TGGCACCTGG 
ACAGGCCATG GCCCTTCCAT GGAGGATTCC TCTGCTGTCC TCATGGAAAG CCTAAAGTCT 
ATCTGGTCCA GTTCCATGAT GCATCCTGGA CCTTCTGCTC TGGAGCAGCT GTTAATGCAG 
CAGAAGCAGA AACAGCAACG GGGACAAGGC ACCATGAACC CTCCACACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG7 ( XM_005245652.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005245709.1 Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X3, mRNA.

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CloneID	OHu34882
Clone ID Related Accession (Same CDS sequence)	XM_005245652.3 , NM_001375584.1
Accession Version	XM_005245652.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3564bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-12
Organism	Homo sapiens(human)
Product	protein SMG7 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005245652.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20190607 Annotation Version :: 109.20190607 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA Annotation Freeze :: 20190607 ##Genome-Annotation-Data-END##

ATGAGCCTGC AGAGCGCGCA GTACCTCCGG CAGGCAGAAG TCCTGAAGGC TGACATGACA 
GATTCTAAGC TGGGTCCAGC TGAAGTCTGG ACATCCAGGC AGGCTCTGCA GGACCTGTAC 
CAGAAAATGC TAGTTACCGA TTTGGAATAC GCTTTAGACA AGAAAGTAGA ACAGGATCTC 
TGGAATCACG CCTTTAAGAA TCAGATCACA ACACTGCAAG GCCAGGCAAA GAATCGAGCA 
AATCCGAATC GGAGTGAAGT TCAGGCAAAC CTTTCTCTGT TCCTAGAGGC AGCTAGTGGC 
TTCTATACTC AGTTATTACA AGAACTGTGT ACAGTATTTA ATGTAGATTT ACCATGCCGT 
GTGAAGTCTT CCCAATTGGG AATTATCAGC AATAAACAGA CGCATACCAG CGCCATAGTG 
AAGCCACAGT CTAGCTCCTG TTCCTATATC TGCCAGCACT GCCTCGTCCA CCTTGGAGAC 
ATTGCTCGAT ACAGAAACCA GACCAGCCAG GCAGAGTCCT ACTATAGGCA TGCAGCTCAG 
CTTGTCCCCT CCAATGGTCA GCCTTATAAT CAGTTGGCTA TCTTAGCTTC TTCCAAAGGA 
GACCATCTGA CCACAATTTT CTACTACTGC AGAAGCATTG CTGTGAAGTT CCCTTTCCCA 
GCTGCCTCCA CTAATCTGCA AAAAGCACTT TCTAAAGCAC TGGAAAGCCG AGATGAGGTG 
AAAACCAAGT GGGGTGTTTC TGACTTCATC AAGGCCTTTA TTAAATTCCA CGGTCATGTG 
TACCTGAGTA AGAGCTTGGA AAAGTTGAGC CCTCTTCGAG AGAAATTGGA AGAACAGTTT 
AAGAGGCTGC TATTCCAAAA AGCTTTCAAC TCTCAGCAGT TAGTTCATGT CACTGTCATT 
AACCTGTTTC AACTTCATCA CCTTCGTGAC TTTAGCAATG AAACCGAGCA GCACACTTAT 
AGCCAAGATG AGCAGCTATG TTGGACACAG TTGCTGGCCC TCTTTATGTC TTTTCTTGGC 
ATCCTGTGCA AGTGTCCTCT ACAGAATGAG TCTCAGGAGG AGTCCTACAA TGCCTATCCT 
CTTCCAGCAG TCAAGGTCTC CATGGACTGG CTAAGACTCA GACCCAGGGT CTTTCAGGAG 
GCAGTGGTGG ATGAAAGACA GTACATTTGG CCCTGGTTGA TTTCTCTTCT GAATAGTTTC 
CATCCCCATG AAGAGGACCT CTCAAGTATT AGTGCGACAC CACTTCCAGA GGAGTTTGAA 
TTACAAGGAT TTTTGGCATT GAGACCTTCT TTCAGGAACT TGGATTTTTC CAAAGGTCAC 
CAGGGTATTA CAGGGGACAA AGAAGGCCAG CAACGACGAA TACGACAGCA ACGCTTGATC 
TCTATAGGCA AATGGATTGC TGATAATCAG CCAAGGCTGA TTCAGTGTGA AAATGAGGTA 
GGGAAATTGT TGTTTATCAC AGAAATCCCA GAATTAATAC TGGAAGACCC CAGTGAAGCC 
AAAGAGAACC TCATTCTGCA AGAAACATCT GTGATAGAGT CGCTGGCTGC AGATGGGAGC 
CCAGGGCTAA AATCAGTGCT ATCTACAAGC CGAAATTTAA GCAACAACTG TGACACAGGA 
GAGAAGCCAG TGGTTACCTT CAAAGAAAAC ATTAAGACAC GAGAAGTGAA CAGAGACCAA 
GGAAGAAGTT TTCCTCCCAA AGAGGTGAGA AGGGACTATA GCAAAGGAAT AACTGTAACT 
AAGAATGATG GAAAGAAGGA CAACAACAAG AGGAAAACTG AAACCAAGAA ATGCACCTTA 
GAAAAGTTAC AGGAAACAGG AAAGCAGAAT GTGGCAGTGC AGGTAAAATC CCAGACAGAA 
CTAAGAAAGA CTCCAGTGTC TGAAGCCAGA AAAACACCTG TAACTCAAAC CCCAACTCAA 
GCAAGTAACT CCCAGTTCAT CCCCATTCAT CACCCTGGAG CCTTCCCTCC TCTTCCCAGC 
AGGCCAGGGT TTCCGCCCCC AACATATGTT ATCCCCCCGC CTGTGGCATT TTCTATGGGC 
TCAGGTTACA CCTTCCCAGC TGGTGTTTCT GTCCCAGGAA CCTTTCTTCA GCCTACAGCT 
CACTCTCCAG CAGGAAACCA GGTGCAAGCT GGGAAACAGT CCCACATTCC TTACAGCCAG 
CAACGGCCCT CTGGACCAGG GCCAATGAAC CAGGGACCTC AACAATCACA GCCACCTTCC 
CAGCAACCCC TTACATCTTT ACCAGCTCAG CCAACAGCAC AGTCTACAAG CCAGCTGCAG 
GTTCAAGCTC TAACTCAGCA ACAACAATCC CCTACAAAAG CTGTGCCGGC TTTGGGGAAA 
AGCCCGCCTC ACCACTCTGG ATTCCAGCAG TATCAACAGG CAGATGCCTC CAAACAGCTG 
TGGAATCCCC CTCAGGTTCA AGGCCCATTA GGGAAAATTA TGCCTGTGAA ACAGCCCTAC 
TACCTTCAGA CCCAAGACCC CATAAAACTG TTTGAGCCGT CATTGCAACC TCCTGTAATG 
CAGCAGCAGC CTCTAGAAAA AAAAATGAAG CCTTTTCCCA TGGAGCCATA TAACCATAAT 
CCCTCAGAAG TCAAGGTCCC AGAATTCTAC TGGGATTCTT CCTACAGCAT GGCTGATAAC 
AGATCTGTAA TGGCACAGCA AGCAAACATA GACCGCAGGG GCAAACGGTC ACCAGGAGTC 
TTCCGTCCAG AGCAGGATCC TGTACCCAGA ATGCCGTTTG AGGACCCCAA GAGCTCCCCT 
CTGCTTCCTC CGGACCTGTT AAAGAGTCTG GCTGCCTTGG AGGAAGAGGA AGAGCTGATT 
TTTTCTAACC CTCCTGATCT TTACCCGGCT CTGCTGGGGC CTCTCGCCTC TCTTCCTGGA 
CGAAGCCTTT TTAAATCCTT ATTGGAGAAG CCCTCAGAGC TCATGTCACA TTCATCCTCT 
TTCCTGTCCC TCACCGGATT CTCTCTCAAT CAGGAAAGAT ACCCAAATAA TAGTATGTTC 
AATGAGGTAT ATGGGAAAAA CCTGACATCC AGCTCCAAAG CAGAACTCAG TCCCTCAATG 
GCCCCCCAGG AAACATCTCT GTATTCCCTT TTTGAAGGGA CTCCGTGGTC TCCATCACTT 
CCTGCCAGTT CAGATCATTC AACACCAGCC AGCCAGTCTC CTCATTCCTC TAACCCAAGC 
AGCCTACCCA GCTCTCCTCC AACACACAAC CATAATTCTG TTCCATTCTC CAATTTTGGA 
CCCATTGGGA CTCCAGATAA CAGGGATAGA AGGACTGCAG ATCGGTGGAA AACTGATAAG 
CCAGCCATGG GTGGGTTTGG CATTGATTAT CTCTCAGCAA CGTCATCCTC TGAGAGCAGT 
TGGCATCAGG CCAGCACTCC GAGTGGCACC TGGACAGGCC ATGGCCCTTC CATGGAGGAT 
TCCTCTGCTG TCCTCATGGA AAGCCTAAAG TCTATCTGGT CCAGTTCCAT GATGCATCCT 
GGACCTTCTG CTCTGGAGCA GCTGTTAATG CAGCAGAAGC AGAAACAGCA ACGGGGACAA 
GGCACCATGA ACCCTCCACA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005245709.1
CDS	120..3683
Translation	MSLQSAQYLRQAEVLKADMTDSKLGPAEVWTSRQALQDLYQKMLVTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRANPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQTHTSAIVKPQSSSCSYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNLQKALSKALESRDEVKTKWGVSDFIKAFIKFHGHVYLSKSLEKLSPLREKLEEQFKRLLFQKAFNSQQLVHVTVINLFQLHHLRDFSNETEQHTYSQDEQLCWTQLLALFMSFLGILCKCPLQNESQEESYNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPHEEDLSSISATPLPEEFELQGFLALRPSFRNLDFSKGHQGITGDKEGQQRRIRQQRLISIGKWIADNQPRLIQCENEVGKLLFITEIPELILEDPSEAKENLILQETSVIESLAADGSPGLKSVLSTSRNLSNNCDTGEKPVVTFKENIKTREVNRDQGRSFPPKEVRRDYSKGITVTKNDGKKDNNKRKTETKKCTLEKLQETGKQNVAVQVKSQTELRKTPVSEARKTPVTQTPTQASNSQFIPIHHPGAFPPLPSRPGFPPPTYVIPPPVAFSMGSGYTFPAGVSVPGTFLQPTAHSPAGNQVQAGKQSHIPYSQQRPSGPGPMNQGPQQSQPPSQQPLTSLPAQPTAQSTSQLQVQALTQQQQSPTKAVPALGKSPPHHSGFQQYQQADASKQLWNPPQVQGPLGKIMPVKQPYYLQTQDPIKLFEPSLQPPVMQQQPLEKKMKPFPMEPYNHNPSEVKVPEFYWDSSYSMADNRSVMAQQANIDRRGKRSPGVFRPEQDPVPRMPFEDPKSSPLLPPDLLKSLAALEEEEELIFSNPPDLYPALLGPLASLPGRSLFKSLLEKPSELMSHSSSFLSLTGFSLNQERYPNNSMFNEVYGKNLTSSSKAELSPSMAPQETSLYSLFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSVPFSNFGPIGTPDNRDRRTADRWKTDKPAMGGFGIDYLSATSSSESSWHQASTPSGTWTGHGPSMEDSSAVLMESLKSIWSSSMMHPGPSALEQLLMQQKQKQQRGQGTMNPPH

Target ORF information:

RefSeq Version	XM_005245652.3
Organism	Homo sapiens(human)
Definition	Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005245652.3

ORF Insert Sequence:

ATGAGCCTGC AGAGCGCGCA GTACCTCCGG CAGGCAGAAG TCCTGAAGGC TGACATGACA 
GATTCTAAGC TGGGTCCAGC TGAAGTCTGG ACATCCAGGC AGGCTCTGCA GGACCTGTAC 
CAGAAAATGC TAGTTACCGA TTTGGAATAC GCTTTAGACA AGAAAGTAGA ACAGGATCTC 
TGGAATCACG CCTTTAAGAA TCAGATCACA ACACTGCAAG GCCAGGCAAA GAATCGAGCA 
AATCCGAATC GGAGTGAAGT TCAGGCAAAC CTTTCTCTGT TCCTAGAGGC AGCTAGTGGC 
TTCTATACTC AGTTATTACA AGAACTGTGT ACAGTATTTA ATGTAGATTT ACCATGCCGT 
GTGAAGTCTT CCCAATTGGG AATTATCAGC AATAAACAGA CGCATACCAG CGCCATAGTG 
AAGCCACAGT CTAGCTCCTG TTCCTATATC TGCCAGCACT GCCTCGTCCA CCTTGGAGAC 
ATTGCTCGAT ACAGAAACCA GACCAGCCAG GCAGAGTCCT ACTATAGGCA TGCAGCTCAG 
CTTGTCCCCT CCAATGGTCA GCCTTATAAT CAGTTGGCTA TCTTAGCTTC TTCCAAAGGA 
GACCATCTGA CCACAATTTT CTACTACTGC AGAAGCATTG CTGTGAAGTT CCCTTTCCCA 
GCTGCCTCCA CTAATCTGCA AAAAGCACTT TCTAAAGCAC TGGAAAGCCG AGATGAGGTG 
AAAACCAAGT GGGGTGTTTC TGACTTCATC AAGGCCTTTA TTAAATTCCA CGGTCATGTG 
TACCTGAGTA AGAGCTTGGA AAAGTTGAGC CCTCTTCGAG AGAAATTGGA AGAACAGTTT 
AAGAGGCTGC TATTCCAAAA AGCTTTCAAC TCTCAGCAGT TAGTTCATGT CACTGTCATT 
AACCTGTTTC AACTTCATCA CCTTCGTGAC TTTAGCAATG AAACCGAGCA GCACACTTAT 
AGCCAAGATG AGCAGCTATG TTGGACACAG TTGCTGGCCC TCTTTATGTC TTTTCTTGGC 
ATCCTGTGCA AGTGTCCTCT ACAGAATGAG TCTCAGGAGG AGTCCTACAA TGCCTATCCT 
CTTCCAGCAG TCAAGGTCTC CATGGACTGG CTAAGACTCA GACCCAGGGT CTTTCAGGAG 
GCAGTGGTGG ATGAAAGACA GTACATTTGG CCCTGGTTGA TTTCTCTTCT GAATAGTTTC 
CATCCCCATG AAGAGGACCT CTCAAGTATT AGTGCGACAC CACTTCCAGA GGAGTTTGAA 
TTACAAGGAT TTTTGGCATT GAGACCTTCT TTCAGGAACT TGGATTTTTC CAAAGGTCAC 
CAGGGTATTA CAGGGGACAA AGAAGGCCAG CAACGACGAA TACGACAGCA ACGCTTGATC 
TCTATAGGCA AATGGATTGC TGATAATCAG CCAAGGCTGA TTCAGTGTGA AAATGAGGTA 
GGGAAATTGT TGTTTATCAC AGAAATCCCA GAATTAATAC TGGAAGACCC CAGTGAAGCC 
AAAGAGAACC TCATTCTGCA AGAAACATCT GTGATAGAGT CGCTGGCTGC AGATGGGAGC 
CCAGGGCTAA AATCAGTGCT ATCTACAAGC CGAAATTTAA GCAACAACTG TGACACAGGA 
GAGAAGCCAG TGGTTACCTT CAAAGAAAAC ATTAAGACAC GAGAAGTGAA CAGAGACCAA 
GGAAGAAGTT TTCCTCCCAA AGAGGTGAGA AGGGACTATA GCAAAGGAAT AACTGTAACT 
AAGAATGATG GAAAGAAGGA CAACAACAAG AGGAAAACTG AAACCAAGAA ATGCACCTTA 
GAAAAGTTAC AGGAAACAGG AAAGCAGAAT GTGGCAGTGC AGGTAAAATC CCAGACAGAA 
CTAAGAAAGA CTCCAGTGTC TGAAGCCAGA AAAACACCTG TAACTCAAAC CCCAACTCAA 
GCAAGTAACT CCCAGTTCAT CCCCATTCAT CACCCTGGAG CCTTCCCTCC TCTTCCCAGC 
AGGCCAGGGT TTCCGCCCCC AACATATGTT ATCCCCCCGC CTGTGGCATT TTCTATGGGC 
TCAGGTTACA CCTTCCCAGC TGGTGTTTCT GTCCCAGGAA CCTTTCTTCA GCCTACAGCT 
CACTCTCCAG CAGGAAACCA GGTGCAAGCT GGGAAACAGT CCCACATTCC TTACAGCCAG 
CAACGGCCCT CTGGACCAGG GCCAATGAAC CAGGGACCTC AACAATCACA GCCACCTTCC 
CAGCAACCCC TTACATCTTT ACCAGCTCAG CCAACAGCAC AGTCTACAAG CCAGCTGCAG 
GTTCAAGCTC TAACTCAGCA ACAACAATCC CCTACAAAAG CTGTGCCGGC TTTGGGGAAA 
AGCCCGCCTC ACCACTCTGG ATTCCAGCAG TATCAACAGG CAGATGCCTC CAAACAGCTG 
TGGAATCCCC CTCAGGTTCA AGGCCCATTA GGGAAAATTA TGCCTGTGAA ACAGCCCTAC 
TACCTTCAGA CCCAAGACCC CATAAAACTG TTTGAGCCGT CATTGCAACC TCCTGTAATG 
CAGCAGCAGC CTCTAGAAAA AAAAATGAAG CCTTTTCCCA TGGAGCCATA TAACCATAAT 
CCCTCAGAAG TCAAGGTCCC AGAATTCTAC TGGGATTCTT CCTACAGCAT GGCTGATAAC 
AGATCTGTAA TGGCACAGCA AGCAAACATA GACCGCAGGG GCAAACGGTC ACCAGGAGTC 
TTCCGTCCAG AGCAGGATCC TGTACCCAGA ATGCCGTTTG AGGACCCCAA GAGCTCCCCT 
CTGCTTCCTC CGGACCTGTT AAAGAGTCTG GCTGCCTTGG AGGAAGAGGA AGAGCTGATT 
TTTTCTAACC CTCCTGATCT TTACCCGGCT CTGCTGGGGC CTCTCGCCTC TCTTCCTGGA 
CGAAGCCTTT TTAAATCCTT ATTGGAGAAG CCCTCAGAGC TCATGTCACA TTCATCCTCT 
TTCCTGTCCC TCACCGGATT CTCTCTCAAT CAGGAAAGAT ACCCAAATAA TAGTATGTTC 
AATGAGGTAT ATGGGAAAAA CCTGACATCC AGCTCCAAAG CAGAACTCAG TCCCTCAATG 
GCCCCCCAGG AAACATCTCT GTATTCCCTT TTTGAAGGGA CTCCGTGGTC TCCATCACTT 
CCTGCCAGTT CAGATCATTC AACACCAGCC AGCCAGTCTC CTCATTCCTC TAACCCAAGC 
AGCCTACCCA GCTCTCCTCC AACACACAAC CATAATTCTG TTCCATTCTC CAATTTTGGA 
CCCATTGGGA CTCCAGATAA CAGGGATAGA AGGACTGCAG ATCGGTGGAA AACTGATAAG 
CCAGCCATGG GTGGGTTTGG CATTGATTAT CTCTCAGCAA CGTCATCCTC TGAGAGCAGT 
TGGCATCAGG CCAGCACTCC GAGTGGCACC TGGACAGGCC ATGGCCCTTC CATGGAGGAT 
TCCTCTGCTG TCCTCATGGA AAGCCTAAAG TCTATCTGGT CCAGTTCCAT GATGCATCCT 
GGACCTTCTG CTCTGGAGCA GCTGTTAATG CAGCAGAAGC AGAAACAGCA ACGGGGACAA 
GGCACCATGA ACCCTCCACA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG7 ( XM_011510206.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011508508.1 Homo sapiens SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X5, mRNA.

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CloneID	OHu34883
Clone ID Related Accession (Same CDS sequence)	XM_011510206.2 , NM_001331007.2 , NM_001350221.1 , NM_001331007.1 , NM_001350221.2 , XM_011510206.3
Accession Version	XM_011510206.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3513bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	protein SMG7 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011510206.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
CAGATGAGGA CCGAAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG 
GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCTGA AGTCTGGACA 
TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTAG TTACCGATTT GGAATACGCT 
TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCACGCCT TTAAGAATCA GATCACAACA 
CTGCAAGGCC AGGCAAAGAA TCGAGCAAAT CCGAATCGGA GTGAAGTTCA GGCAAACCTT 
TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA 
GTATTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AATTGGGAAT TATCAGCAAT 
AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC 
CAGCACTGCC TCGTCCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA 
GAGTCCTACT ATAGGCATGC AGCTCAGCTT GTCCCCTCCA ATGGTCAGCC TTATAATCAG 
TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACCA CAATTTTCTA CTACTGCAGA 
AGCATTGCTG TGAAGTTCCC TTTCCCAGCT GCCTCCACTA ATCTGCAAAA AGCACTTTCT 
AAAGCACTGG AAAGCCGAGA TGAGGTGAAA ACCAAGTGGG GTGTTTCTGA CTTCATCAAG 
GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT 
CTTCGAGAGA AATTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAAAAAGC TTTCAACTCT 
CAGCAGTTAG TTCATGTCAC TGTCATTAAC CTGTTTCAAC TTCATCACCT TCGTGACTTT 
AGCAATGAAA CCGAGCAGCA CACTTATAGC CAAGATGAGC AGCTATGTTG GACACAGTTG 
CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTACA GAATGAGTCT 
CAGGAGGAGT CCTACAATGC CTATCCTCTT CCAGCAGTCA AGGTCTCCAT GGACTGGCTA 
AGACTCAGAC CCAGGGTCTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCC 
TGGTTGATTT CTCTTCTGAA TAGTTTCCAT CCCCATGAAG AGGACCTCTC AAGTATTAGT 
GCGACACCAC TTCCAGAGGA GTTTGAATTA CAAGGATTTT TGGCATTGAG ACCTTCTTTC 
AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GGGACAAAGA AGGCCAGCAA 
CGACGAATAC GACAGCAACG CTTGATCTCT ATAGGCAAAT GGATTGCTGA TAATCAGCCA 
AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAA 
TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA AACATCTGTG 
ATAGAGTCGC TGGCTGCAGA TGGGAGCCCA GGGCTAAAAT CAGTGCTATC TACAAGCCGA 
AATTTAAGCA ACAACTGTGA CACAGGAGAG AAGCCAGTGG TTACCTTCAA AGAAAACATT 
AAGACACGAG AAGTGAACAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTAAAATCC 
CAGACAGAAC TAAGAAAGAC TCCAGTGTCT GAAGCCAGAA AAACACCTGT AACTCAAACC 
CCAACTCAAG CAAGTAACTC CCAGTTCATC CCCATTCATC ACCCTGGAGC CTTCCCTCCT 
CTTCCCAGCA GGCCAGGGTT TCCGCCCCCA ACATATGTTA TCCCCCCGCC TGTGGCATTT 
TCTATGGGCT CAGGTTACAC CTTCCCAGCT GGTGTTTCTG TCCCAGGAAC CTTTCTTCAG 
CCTACAGCTC ACTCTCCAGC AGGAAACCAG GTGCAAGCTG GGAAACAGTC CCACATTCCT 
TACAGCCAGC AACGGCCCTC TGGACCAGGG CCAATGAACC AGGGACCTCA ACAATCACAG 
CCACCTTCCC AGCAACCCCT TACATCTTTA CCAGCTCAGC CAACAGCACA GTCTACAAGC 
CAGCTGCAGG TTCAAGCTCT AACTCAGCAA CAACAATCCC CTACAAAAGC TGTGCCGGCT 
TTGGGGAAAA GCCCGCCTCA CCACTCTGGA TTCCAGCAGT ATCAACAGGC AGATGCCTCC 
AAACAGCTGT GGAATCCCCC TCAGGTTCAA GGCCCATTAG GGAAAATTAT GCCTGTGAAA 
CAGCCCTACT ACCTTCAGAC CCAAGACCCC ATAAAACTGT TTGAGCCGTC ATTGCAACCT 
CCTGTAATGC AGCAGCAGCC TCTAGAAAAA AAAATGAAGC CTTTTCCCAT GGAGCCATAT 
AACCATAATC CCTCAGAAGT CAAGGTCCCA GAATTCTACT GGGATTCTTC CTACAGCATG 
GCTGATAACA GATCTGTAAT GGCACAGCAA GCAAACATAG ACCGCAGGGG CAAACGGTCA 
CCAGGAGTCT TCCGTCCAGA GCAGGATCCT GTACCCAGAA TGCCGTTTGA GGACCCCAAG 
AGCTCCCCTC TGCTTCCTCC GGACCTGTTA AAGAGTCTGG CTGCCTTGGA GGAAGAGGAA 
GAGCTGATTT TTTCTAACCC TCCTGATCTT TACCCGGCTC TGCTGGGGCC TCTCGCCTCT 
CTTCCTGGAC GAAGCCTTTT TAAATCCTTA TTGGAGAAGC CCTCAGAGCT CATGTCACAT 
TCATCCTCTT TCCTGTCCCT CACCGGATTC TCTCTCAATC AGGAAAGATA CCCAAATAAT 
AGTATGTTCA ATGAGGTATA TGGGAAAAAC CTGACATCCA GCTCCAAAGC AGAACTCAGT 
CCCTCAATGG CCCCCCAGGA AACATCTCTG TATTCCCTTT TTGAAGGGAC TCCGTGGTCT 
CCATCACTTC CTGCCAGTTC AGATCATTCA ACACCAGCCA GCCAGTCTCC TCATTCCTCT 
AACCCAAGCA GCCTACCCAG CTCTCCTCCA ACACACAACC ATAATTCTGT TCCATTCTCC 
AATTTTGGAC CCATTGGGAC TCCAGATAAC AGGGATAGAA GGACTGCAGA TCGGTGGAAA 
ACTGATAAGC CAGCCATGGG TGGGTTTGGC ATTGATTATC TCTCAGCAAC GTCATCCTCT 
GAGAGCAGTT GGCATCAGGC CAGCACTCCG AGTGGCACCT GGACAGGCCA TGGCCCTTCC 
ATGGAGGATT CCTCTGCTGT CCTCATGGAA AGCCTAAAGT CTATCTGGTC CAGTTCCATG 
ATGCATCCTG GACCTTCTGC TCTGGAGCAG CTGTTAATGC AGCAGAAGCA GAAACAGCAA 
CGGGGACAAG GCACCATGAA CCCTCCACAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011508508.1
CDS	1027..4539
Translation	MRNWMFLQIVIVGENVSFKSQMRTENLKSEEHLKSSNIRQAEVLKADMTDSKLGPAEVWTSRQALQDLYQKMLVTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRANPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQTHTSAIVKPQSSSCSYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNLQKALSKALESRDEVKTKWGVSDFIKAFIKFHGHVYLSKSLEKLSPLREKLEEQFKRLLFQKAFNSQQLVHVTVINLFQLHHLRDFSNETEQHTYSQDEQLCWTQLLALFMSFLGILCKCPLQNESQEESYNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPHEEDLSSISATPLPEEFELQGFLALRPSFRNLDFSKGHQGITGDKEGQQRRIRQQRLISIGKWIADNQPRLIQCENEVGKLLFITEIPELILEDPSEAKENLILQETSVIESLAADGSPGLKSVLSTSRNLSNNCDTGEKPVVTFKENIKTREVNRDQGRSFPPKEVKSQTELRKTPVSEARKTPVTQTPTQASNSQFIPIHHPGAFPPLPSRPGFPPPTYVIPPPVAFSMGSGYTFPAGVSVPGTFLQPTAHSPAGNQVQAGKQSHIPYSQQRPSGPGPMNQGPQQSQPPSQQPLTSLPAQPTAQSTSQLQVQALTQQQQSPTKAVPALGKSPPHHSGFQQYQQADASKQLWNPPQVQGPLGKIMPVKQPYYLQTQDPIKLFEPSLQPPVMQQQPLEKKMKPFPMEPYNHNPSEVKVPEFYWDSSYSMADNRSVMAQQANIDRRGKRSPGVFRPEQDPVPRMPFEDPKSSPLLPPDLLKSLAALEEEEELIFSNPPDLYPALLGPLASLPGRSLFKSLLEKPSELMSHSSSFLSLTGFSLNQERYPNNSMFNEVYGKNLTSSSKAELSPSMAPQETSLYSLFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSVPFSNFGPIGTPDNRDRRTADRWKTDKPAMGGFGIDYLSATSSSESSWHQASTPSGTWTGHGPSMEDSSAVLMESLKSIWSSSMMHPGPSALEQLLMQQKQKQQRGQGTMNPPH

Target ORF information:

RefSeq Version	XM_011510206.2
Organism	Homo sapiens(human)
Definition	Homo sapiens SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011510206.2

ORF Insert Sequence:

ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
CAGATGAGGA CCGAAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG 
GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCTGA AGTCTGGACA 
TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTAG TTACCGATTT GGAATACGCT 
TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCACGCCT TTAAGAATCA GATCACAACA 
CTGCAAGGCC AGGCAAAGAA TCGAGCAAAT CCGAATCGGA GTGAAGTTCA GGCAAACCTT 
TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA 
GTATTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AATTGGGAAT TATCAGCAAT 
AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC 
CAGCACTGCC TCGTCCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA 
GAGTCCTACT ATAGGCATGC AGCTCAGCTT GTCCCCTCCA ATGGTCAGCC TTATAATCAG 
TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACCA CAATTTTCTA CTACTGCAGA 
AGCATTGCTG TGAAGTTCCC TTTCCCAGCT GCCTCCACTA ATCTGCAAAA AGCACTTTCT 
AAAGCACTGG AAAGCCGAGA TGAGGTGAAA ACCAAGTGGG GTGTTTCTGA CTTCATCAAG 
GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT 
CTTCGAGAGA AATTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAAAAAGC TTTCAACTCT 
CAGCAGTTAG TTCATGTCAC TGTCATTAAC CTGTTTCAAC TTCATCACCT TCGTGACTTT 
AGCAATGAAA CCGAGCAGCA CACTTATAGC CAAGATGAGC AGCTATGTTG GACACAGTTG 
CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTACA GAATGAGTCT 
CAGGAGGAGT CCTACAATGC CTATCCTCTT CCAGCAGTCA AGGTCTCCAT GGACTGGCTA 
AGACTCAGAC CCAGGGTCTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCC 
TGGTTGATTT CTCTTCTGAA TAGTTTCCAT CCCCATGAAG AGGACCTCTC AAGTATTAGT 
GCGACACCAC TTCCAGAGGA GTTTGAATTA CAAGGATTTT TGGCATTGAG ACCTTCTTTC 
AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GGGACAAAGA AGGCCAGCAA 
CGACGAATAC GACAGCAACG CTTGATCTCT ATAGGCAAAT GGATTGCTGA TAATCAGCCA 
AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAA 
TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA AACATCTGTG 
ATAGAGTCGC TGGCTGCAGA TGGGAGCCCA GGGCTAAAAT CAGTGCTATC TACAAGCCGA 
AATTTAAGCA ACAACTGTGA CACAGGAGAG AAGCCAGTGG TTACCTTCAA AGAAAACATT 
AAGACACGAG AAGTGAACAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTAAAATCC 
CAGACAGAAC TAAGAAAGAC TCCAGTGTCT GAAGCCAGAA AAACACCTGT AACTCAAACC 
CCAACTCAAG CAAGTAACTC CCAGTTCATC CCCATTCATC ACCCTGGAGC CTTCCCTCCT 
CTTCCCAGCA GGCCAGGGTT TCCGCCCCCA ACATATGTTA TCCCCCCGCC TGTGGCATTT 
TCTATGGGCT CAGGTTACAC CTTCCCAGCT GGTGTTTCTG TCCCAGGAAC CTTTCTTCAG 
CCTACAGCTC ACTCTCCAGC AGGAAACCAG GTGCAAGCTG GGAAACAGTC CCACATTCCT 
TACAGCCAGC AACGGCCCTC TGGACCAGGG CCAATGAACC AGGGACCTCA ACAATCACAG 
CCACCTTCCC AGCAACCCCT TACATCTTTA CCAGCTCAGC CAACAGCACA GTCTACAAGC 
CAGCTGCAGG TTCAAGCTCT AACTCAGCAA CAACAATCCC CTACAAAAGC TGTGCCGGCT 
TTGGGGAAAA GCCCGCCTCA CCACTCTGGA TTCCAGCAGT ATCAACAGGC AGATGCCTCC 
AAACAGCTGT GGAATCCCCC TCAGGTTCAA GGCCCATTAG GGAAAATTAT GCCTGTGAAA 
CAGCCCTACT ACCTTCAGAC CCAAGACCCC ATAAAACTGT TTGAGCCGTC ATTGCAACCT 
CCTGTAATGC AGCAGCAGCC TCTAGAAAAA AAAATGAAGC CTTTTCCCAT GGAGCCATAT 
AACCATAATC CCTCAGAAGT CAAGGTCCCA GAATTCTACT GGGATTCTTC CTACAGCATG 
GCTGATAACA GATCTGTAAT GGCACAGCAA GCAAACATAG ACCGCAGGGG CAAACGGTCA 
CCAGGAGTCT TCCGTCCAGA GCAGGATCCT GTACCCAGAA TGCCGTTTGA GGACCCCAAG 
AGCTCCCCTC TGCTTCCTCC GGACCTGTTA AAGAGTCTGG CTGCCTTGGA GGAAGAGGAA 
GAGCTGATTT TTTCTAACCC TCCTGATCTT TACCCGGCTC TGCTGGGGCC TCTCGCCTCT 
CTTCCTGGAC GAAGCCTTTT TAAATCCTTA TTGGAGAAGC CCTCAGAGCT CATGTCACAT 
TCATCCTCTT TCCTGTCCCT CACCGGATTC TCTCTCAATC AGGAAAGATA CCCAAATAAT 
AGTATGTTCA ATGAGGTATA TGGGAAAAAC CTGACATCCA GCTCCAAAGC AGAACTCAGT 
CCCTCAATGG CCCCCCAGGA AACATCTCTG TATTCCCTTT TTGAAGGGAC TCCGTGGTCT 
CCATCACTTC CTGCCAGTTC AGATCATTCA ACACCAGCCA GCCAGTCTCC TCATTCCTCT 
AACCCAAGCA GCCTACCCAG CTCTCCTCCA ACACACAACC ATAATTCTGT TCCATTCTCC 
AATTTTGGAC CCATTGGGAC TCCAGATAAC AGGGATAGAA GGACTGCAGA TCGGTGGAAA 
ACTGATAAGC CAGCCATGGG TGGGTTTGGC ATTGATTATC TCTCAGCAAC GTCATCCTCT 
GAGAGCAGTT GGCATCAGGC CAGCACTCCG AGTGGCACCT GGACAGGCCA TGGCCCTTCC 
ATGGAGGATT CCTCTGCTGT CCTCATGGAA AGCCTAAAGT CTATCTGGTC CAGTTCCATG 
ATGCATCCTG GACCTTCTGC TCTGGAGCAG CTGTTAATGC AGCAGAAGCA GAAACAGCAA 
CGGGGACAAG GCACCATGAA CCCTCCACAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG7 ( XM_011510206.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011508508.1 Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X4, mRNA.

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CloneID	OHu34883
Clone ID Related Accession (Same CDS sequence)	XM_011510206.2 , NM_001331007.2 , NM_001350221.1 , NM_001331007.1 , NM_001350221.2 , XM_011510206.3
Accession Version	XM_011510206.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3513bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein SMG7 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011510206.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
CAGATGAGGA CCGAAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG 
GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCTGA AGTCTGGACA 
TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTAG TTACCGATTT GGAATACGCT 
TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCACGCCT TTAAGAATCA GATCACAACA 
CTGCAAGGCC AGGCAAAGAA TCGAGCAAAT CCGAATCGGA GTGAAGTTCA GGCAAACCTT 
TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA 
GTATTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AATTGGGAAT TATCAGCAAT 
AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC 
CAGCACTGCC TCGTCCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA 
GAGTCCTACT ATAGGCATGC AGCTCAGCTT GTCCCCTCCA ATGGTCAGCC TTATAATCAG 
TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACCA CAATTTTCTA CTACTGCAGA 
AGCATTGCTG TGAAGTTCCC TTTCCCAGCT GCCTCCACTA ATCTGCAAAA AGCACTTTCT 
AAAGCACTGG AAAGCCGAGA TGAGGTGAAA ACCAAGTGGG GTGTTTCTGA CTTCATCAAG 
GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT 
CTTCGAGAGA AATTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAAAAAGC TTTCAACTCT 
CAGCAGTTAG TTCATGTCAC TGTCATTAAC CTGTTTCAAC TTCATCACCT TCGTGACTTT 
AGCAATGAAA CCGAGCAGCA CACTTATAGC CAAGATGAGC AGCTATGTTG GACACAGTTG 
CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTACA GAATGAGTCT 
CAGGAGGAGT CCTACAATGC CTATCCTCTT CCAGCAGTCA AGGTCTCCAT GGACTGGCTA 
AGACTCAGAC CCAGGGTCTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCC 
TGGTTGATTT CTCTTCTGAA TAGTTTCCAT CCCCATGAAG AGGACCTCTC AAGTATTAGT 
GCGACACCAC TTCCAGAGGA GTTTGAATTA CAAGGATTTT TGGCATTGAG ACCTTCTTTC 
AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GGGACAAAGA AGGCCAGCAA 
CGACGAATAC GACAGCAACG CTTGATCTCT ATAGGCAAAT GGATTGCTGA TAATCAGCCA 
AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAA 
TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA AACATCTGTG 
ATAGAGTCGC TGGCTGCAGA TGGGAGCCCA GGGCTAAAAT CAGTGCTATC TACAAGCCGA 
AATTTAAGCA ACAACTGTGA CACAGGAGAG AAGCCAGTGG TTACCTTCAA AGAAAACATT 
AAGACACGAG AAGTGAACAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTAAAATCC 
CAGACAGAAC TAAGAAAGAC TCCAGTGTCT GAAGCCAGAA AAACACCTGT AACTCAAACC 
CCAACTCAAG CAAGTAACTC CCAGTTCATC CCCATTCATC ACCCTGGAGC CTTCCCTCCT 
CTTCCCAGCA GGCCAGGGTT TCCGCCCCCA ACATATGTTA TCCCCCCGCC TGTGGCATTT 
TCTATGGGCT CAGGTTACAC CTTCCCAGCT GGTGTTTCTG TCCCAGGAAC CTTTCTTCAG 
CCTACAGCTC ACTCTCCAGC AGGAAACCAG GTGCAAGCTG GGAAACAGTC CCACATTCCT 
TACAGCCAGC AACGGCCCTC TGGACCAGGG CCAATGAACC AGGGACCTCA ACAATCACAG 
CCACCTTCCC AGCAACCCCT TACATCTTTA CCAGCTCAGC CAACAGCACA GTCTACAAGC 
CAGCTGCAGG TTCAAGCTCT AACTCAGCAA CAACAATCCC CTACAAAAGC TGTGCCGGCT 
TTGGGGAAAA GCCCGCCTCA CCACTCTGGA TTCCAGCAGT ATCAACAGGC AGATGCCTCC 
AAACAGCTGT GGAATCCCCC TCAGGTTCAA GGCCCATTAG GGAAAATTAT GCCTGTGAAA 
CAGCCCTACT ACCTTCAGAC CCAAGACCCC ATAAAACTGT TTGAGCCGTC ATTGCAACCT 
CCTGTAATGC AGCAGCAGCC TCTAGAAAAA AAAATGAAGC CTTTTCCCAT GGAGCCATAT 
AACCATAATC CCTCAGAAGT CAAGGTCCCA GAATTCTACT GGGATTCTTC CTACAGCATG 
GCTGATAACA GATCTGTAAT GGCACAGCAA GCAAACATAG ACCGCAGGGG CAAACGGTCA 
CCAGGAGTCT TCCGTCCAGA GCAGGATCCT GTACCCAGAA TGCCGTTTGA GGACCCCAAG 
AGCTCCCCTC TGCTTCCTCC GGACCTGTTA AAGAGTCTGG CTGCCTTGGA GGAAGAGGAA 
GAGCTGATTT TTTCTAACCC TCCTGATCTT TACCCGGCTC TGCTGGGGCC TCTCGCCTCT 
CTTCCTGGAC GAAGCCTTTT TAAATCCTTA TTGGAGAAGC CCTCAGAGCT CATGTCACAT 
TCATCCTCTT TCCTGTCCCT CACCGGATTC TCTCTCAATC AGGAAAGATA CCCAAATAAT 
AGTATGTTCA ATGAGGTATA TGGGAAAAAC CTGACATCCA GCTCCAAAGC AGAACTCAGT 
CCCTCAATGG CCCCCCAGGA AACATCTCTG TATTCCCTTT TTGAAGGGAC TCCGTGGTCT 
CCATCACTTC CTGCCAGTTC AGATCATTCA ACACCAGCCA GCCAGTCTCC TCATTCCTCT 
AACCCAAGCA GCCTACCCAG CTCTCCTCCA ACACACAACC ATAATTCTGT TCCATTCTCC 
AATTTTGGAC CCATTGGGAC TCCAGATAAC AGGGATAGAA GGACTGCAGA TCGGTGGAAA 
ACTGATAAGC CAGCCATGGG TGGGTTTGGC ATTGATTATC TCTCAGCAAC GTCATCCTCT 
GAGAGCAGTT GGCATCAGGC CAGCACTCCG AGTGGCACCT GGACAGGCCA TGGCCCTTCC 
ATGGAGGATT CCTCTGCTGT CCTCATGGAA AGCCTAAAGT CTATCTGGTC CAGTTCCATG 
ATGCATCCTG GACCTTCTGC TCTGGAGCAG CTGTTAATGC AGCAGAAGCA GAAACAGCAA 
CGGGGACAAG GCACCATGAA CCCTCCACAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011508508.1
CDS	996..4508
Translation	MRNWMFLQIVIVGENVSFKSQMRTENLKSEEHLKSSNIRQAEVLKADMTDSKLGPAEVWTSRQALQDLYQKMLVTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRANPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQTHTSAIVKPQSSSCSYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNLQKALSKALESRDEVKTKWGVSDFIKAFIKFHGHVYLSKSLEKLSPLREKLEEQFKRLLFQKAFNSQQLVHVTVINLFQLHHLRDFSNETEQHTYSQDEQLCWTQLLALFMSFLGILCKCPLQNESQEESYNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPHEEDLSSISATPLPEEFELQGFLALRPSFRNLDFSKGHQGITGDKEGQQRRIRQQRLISIGKWIADNQPRLIQCENEVGKLLFITEIPELILEDPSEAKENLILQETSVIESLAADGSPGLKSVLSTSRNLSNNCDTGEKPVVTFKENIKTREVNRDQGRSFPPKEVKSQTELRKTPVSEARKTPVTQTPTQASNSQFIPIHHPGAFPPLPSRPGFPPPTYVIPPPVAFSMGSGYTFPAGVSVPGTFLQPTAHSPAGNQVQAGKQSHIPYSQQRPSGPGPMNQGPQQSQPPSQQPLTSLPAQPTAQSTSQLQVQALTQQQQSPTKAVPALGKSPPHHSGFQQYQQADASKQLWNPPQVQGPLGKIMPVKQPYYLQTQDPIKLFEPSLQPPVMQQQPLEKKMKPFPMEPYNHNPSEVKVPEFYWDSSYSMADNRSVMAQQANIDRRGKRSPGVFRPEQDPVPRMPFEDPKSSPLLPPDLLKSLAALEEEEELIFSNPPDLYPALLGPLASLPGRSLFKSLLEKPSELMSHSSSFLSLTGFSLNQERYPNNSMFNEVYGKNLTSSSKAELSPSMAPQETSLYSLFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSVPFSNFGPIGTPDNRDRRTADRWKTDKPAMGGFGIDYLSATSSSESSWHQASTPSGTWTGHGPSMEDSSAVLMESLKSIWSSSMMHPGPSALEQLLMQQKQKQQRGQGTMNPPH

Target ORF information:

RefSeq Version	XM_011510206.3
Organism	Homo sapiens(human)
Definition	Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011510206.3

ORF Insert Sequence:

ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
CAGATGAGGA CCGAAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG 
GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCTGA AGTCTGGACA 
TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTAG TTACCGATTT GGAATACGCT 
TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCACGCCT TTAAGAATCA GATCACAACA 
CTGCAAGGCC AGGCAAAGAA TCGAGCAAAT CCGAATCGGA GTGAAGTTCA GGCAAACCTT 
TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA 
GTATTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AATTGGGAAT TATCAGCAAT 
AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC 
CAGCACTGCC TCGTCCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA 
GAGTCCTACT ATAGGCATGC AGCTCAGCTT GTCCCCTCCA ATGGTCAGCC TTATAATCAG 
TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACCA CAATTTTCTA CTACTGCAGA 
AGCATTGCTG TGAAGTTCCC TTTCCCAGCT GCCTCCACTA ATCTGCAAAA AGCACTTTCT 
AAAGCACTGG AAAGCCGAGA TGAGGTGAAA ACCAAGTGGG GTGTTTCTGA CTTCATCAAG 
GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT 
CTTCGAGAGA AATTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAAAAAGC TTTCAACTCT 
CAGCAGTTAG TTCATGTCAC TGTCATTAAC CTGTTTCAAC TTCATCACCT TCGTGACTTT 
AGCAATGAAA CCGAGCAGCA CACTTATAGC CAAGATGAGC AGCTATGTTG GACACAGTTG 
CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTACA GAATGAGTCT 
CAGGAGGAGT CCTACAATGC CTATCCTCTT CCAGCAGTCA AGGTCTCCAT GGACTGGCTA 
AGACTCAGAC CCAGGGTCTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCC 
TGGTTGATTT CTCTTCTGAA TAGTTTCCAT CCCCATGAAG AGGACCTCTC AAGTATTAGT 
GCGACACCAC TTCCAGAGGA GTTTGAATTA CAAGGATTTT TGGCATTGAG ACCTTCTTTC 
AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GGGACAAAGA AGGCCAGCAA 
CGACGAATAC GACAGCAACG CTTGATCTCT ATAGGCAAAT GGATTGCTGA TAATCAGCCA 
AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAA 
TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA AACATCTGTG 
ATAGAGTCGC TGGCTGCAGA TGGGAGCCCA GGGCTAAAAT CAGTGCTATC TACAAGCCGA 
AATTTAAGCA ACAACTGTGA CACAGGAGAG AAGCCAGTGG TTACCTTCAA AGAAAACATT 
AAGACACGAG AAGTGAACAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTAAAATCC 
CAGACAGAAC TAAGAAAGAC TCCAGTGTCT GAAGCCAGAA AAACACCTGT AACTCAAACC 
CCAACTCAAG CAAGTAACTC CCAGTTCATC CCCATTCATC ACCCTGGAGC CTTCCCTCCT 
CTTCCCAGCA GGCCAGGGTT TCCGCCCCCA ACATATGTTA TCCCCCCGCC TGTGGCATTT 
TCTATGGGCT CAGGTTACAC CTTCCCAGCT GGTGTTTCTG TCCCAGGAAC CTTTCTTCAG 
CCTACAGCTC ACTCTCCAGC AGGAAACCAG GTGCAAGCTG GGAAACAGTC CCACATTCCT 
TACAGCCAGC AACGGCCCTC TGGACCAGGG CCAATGAACC AGGGACCTCA ACAATCACAG 
CCACCTTCCC AGCAACCCCT TACATCTTTA CCAGCTCAGC CAACAGCACA GTCTACAAGC 
CAGCTGCAGG TTCAAGCTCT AACTCAGCAA CAACAATCCC CTACAAAAGC TGTGCCGGCT 
TTGGGGAAAA GCCCGCCTCA CCACTCTGGA TTCCAGCAGT ATCAACAGGC AGATGCCTCC 
AAACAGCTGT GGAATCCCCC TCAGGTTCAA GGCCCATTAG GGAAAATTAT GCCTGTGAAA 
CAGCCCTACT ACCTTCAGAC CCAAGACCCC ATAAAACTGT TTGAGCCGTC ATTGCAACCT 
CCTGTAATGC AGCAGCAGCC TCTAGAAAAA AAAATGAAGC CTTTTCCCAT GGAGCCATAT 
AACCATAATC CCTCAGAAGT CAAGGTCCCA GAATTCTACT GGGATTCTTC CTACAGCATG 
GCTGATAACA GATCTGTAAT GGCACAGCAA GCAAACATAG ACCGCAGGGG CAAACGGTCA 
CCAGGAGTCT TCCGTCCAGA GCAGGATCCT GTACCCAGAA TGCCGTTTGA GGACCCCAAG 
AGCTCCCCTC TGCTTCCTCC GGACCTGTTA AAGAGTCTGG CTGCCTTGGA GGAAGAGGAA 
GAGCTGATTT TTTCTAACCC TCCTGATCTT TACCCGGCTC TGCTGGGGCC TCTCGCCTCT 
CTTCCTGGAC GAAGCCTTTT TAAATCCTTA TTGGAGAAGC CCTCAGAGCT CATGTCACAT 
TCATCCTCTT TCCTGTCCCT CACCGGATTC TCTCTCAATC AGGAAAGATA CCCAAATAAT 
AGTATGTTCA ATGAGGTATA TGGGAAAAAC CTGACATCCA GCTCCAAAGC AGAACTCAGT 
CCCTCAATGG CCCCCCAGGA AACATCTCTG TATTCCCTTT TTGAAGGGAC TCCGTGGTCT 
CCATCACTTC CTGCCAGTTC AGATCATTCA ACACCAGCCA GCCAGTCTCC TCATTCCTCT 
AACCCAAGCA GCCTACCCAG CTCTCCTCCA ACACACAACC ATAATTCTGT TCCATTCTCC 
AATTTTGGAC CCATTGGGAC TCCAGATAAC AGGGATAGAA GGACTGCAGA TCGGTGGAAA 
ACTGATAAGC CAGCCATGGG TGGGTTTGGC ATTGATTATC TCTCAGCAAC GTCATCCTCT 
GAGAGCAGTT GGCATCAGGC CAGCACTCCG AGTGGCACCT GGACAGGCCA TGGCCCTTCC 
ATGGAGGATT CCTCTGCTGT CCTCATGGAA AGCCTAAAGT CTATCTGGTC CAGTTCCATG 
ATGCATCCTG GACCTTCTGC TCTGGAGCAG CTGTTAATGC AGCAGAAGCA GAAACAGCAA 
CGGGGACAAG GCACCATGAA CCCTCCACAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG7 ( XM_011510207.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011508509.1 Homo sapiens SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X7, mRNA.

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CloneID	OHu34884
Clone ID Related Accession (Same CDS sequence)	XM_011510207.2 , XM_011510207.3 , XM_005245653.4 , XM_005245653.5
Accession Version	XM_011510207.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3510bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	protein SMG7 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011510207.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAGATT CTAAGCTGGG TCCAGCTGAA GTCTGGACAT CCAGGCAGGC TCTGCAGGAC 
CTGTACCAGA AAATGCTAGT TACCGATTTG GAATACGCTT TAGACAAGAA AGTAGAACAG 
GATCTCTGGA ATCACGCCTT TAAGAATCAG ATCACAACAC TGCAAGGCCA GGCAAAGAAT 
CGAGCAAATC CGAATCGGAG TGAAGTTCAG GCAAACCTTT CTCTGTTCCT AGAGGCAGCT 
AGTGGCTTCT ATACTCAGTT ATTACAAGAA CTGTGTACAG TATTTAATGT AGATTTACCA 
TGCCGTGTGA AGTCTTCCCA ATTGGGAATT ATCAGCAATA AACAGACGCA TACCAGCGCC 
ATAGTGAAGC CACAGTCTAG CTCCTGTTCC TATATCTGCC AGCACTGCCT CGTCCACCTT 
GGAGACATTG CTCGATACAG AAACCAGACC AGCCAGGCAG AGTCCTACTA TAGGCATGCA 
GCTCAGCTTG TCCCCTCCAA TGGTCAGCCT TATAATCAGT TGGCTATCTT AGCTTCTTCC 
AAAGGAGACC ATCTGACCAC AATTTTCTAC TACTGCAGAA GCATTGCTGT GAAGTTCCCT 
TTCCCAGCTG CCTCCACTAA TCTGCAAAAA GCACTTTCTA AAGCACTGGA AAGCCGAGAT 
GAGGTGAAAA CCAAGTGGGG TGTTTCTGAC TTCATCAAGG CCTTTATTAA ATTCCACGGT 
CATGTGTACC TGAGTAAGAG CTTGGAAAAG TTGAGCCCTC TTCGAGAGAA ATTGGAAGAA 
CAGTTTAAGA GGCTGCTATT CCAAAAAGCT TTCAACTCTC AGCAGTTAGT TCATGTCACT 
GTCATTAACC TGTTTCAACT TCATCACCTT CGTGACTTTA GCAATGAAAC CGAGCAGCAC 
ACTTATAGCC AAGATGAGCA GCTATGTTGG ACACAGTTGC TGGCCCTCTT TATGTCTTTT 
CTTGGCATCC TGTGCAAGTG TCCTCTACAG AATGAGTCTC AGGAGGAGTC CTACAATGCC 
TATCCTCTTC CAGCAGTCAA GGTCTCCATG GACTGGCTAA GACTCAGACC CAGGGTCTTT 
CAGGAGGCAG TGGTGGATGA AAGACAGTAC ATTTGGCCCT GGTTGATTTC TCTTCTGAAT 
AGTTTCCATC CCCATGAAGA GGACCTCTCA AGTATTAGTG CGACACCACT TCCAGAGGAG 
TTTGAATTAC AAGGATTTTT GGCATTGAGA CCTTCTTTCA GGAACTTGGA TTTTTCCAAA 
GGTCACCAGG GTATTACAGG GGACAAAGAA GGCCAGCAAC GACGAATACG ACAGCAACGC 
TTGATCTCTA TAGGCAAATG GATTGCTGAT AATCAGCCAA GGCTGATTCA GTGTGAAAAT 
GAGGTAGGGA AATTGTTGTT TATCACAGAA ATCCCAGAAT TAATACTGGA AGACCCCAGT 
GAAGCCAAAG AGAACCTCAT TCTGCAAGAA ACATCTGTGA TAGAGTCGCT GGCTGCAGAT 
GGGAGCCCAG GGCTAAAATC AGTGCTATCT ACAAGCCGAA ATTTAAGCAA CAACTGTGAC 
ACAGGAGAGA AGCCAGTGGT TACCTTCAAA GAAAACATTA AGACACGAGA AGTGAACAGA 
GACCAAGGAA GAAGTTTTCC TCCCAAAGAG GTGAGAAGGG ACTATAGCAA AGGAATAACT 
GTAACTAAGA ATGATGGAAA GAAGGACAAC AACAAGAGGA AAACTGAAAC CAAGAAATGC 
ACCTTAGAAA AGTTACAGGA AACAGGAAAG CAGAATGTGG CAGTGCAGGT AAAATCCCAG 
ACAGAACTAA GAAAGACTCC AGTGTCTGAA GCCAGAAAAA CACCTGTAAC TCAAACCCCA 
ACTCAAGCAA GTAACTCCCA GTTCATCCCC ATTCATCACC CTGGAGCCTT CCCTCCTCTT 
CCCAGCAGGC CAGGGTTTCC GCCCCCAACA TATGTTATCC CCCCGCCTGT GGCATTTTCT 
ATGGGCTCAG GTTACACCTT CCCAGCTGGT GTTTCTGTCC CAGGAACCTT TCTTCAGCCT 
ACAGCTCACT CTCCAGCAGG AAACCAGGTG CAAGCTGGGA AACAGTCCCA CATTCCTTAC 
AGCCAGCAAC GGCCCTCTGG ACCAGGGCCA ATGAACCAGG GACCTCAACA ATCACAGCCA 
CCTTCCCAGC AACCCCTTAC ATCTTTACCA GCTCAGCCAA CAGCACAGTC TACAAGCCAG 
CTGCAGGTTC AAGCTCTAAC TCAGCAACAA CAATCCCCTA CAAAAGCTGT GCCGGCTTTG 
GGGAAAAGCC CGCCTCACCA CTCTGGATTC CAGCAGTATC AACAGGCAGA TGCCTCCAAA 
CAGCTGTGGA ATCCCCCTCA GGTTCAAGGC CCATTAGGGA AAATTATGCC TGTGAAACAG 
CCCTACTACC TTCAGACCCA AGACCCCATA AAACTGTTTG AGCCGTCATT GCAACCTCCT 
GTAATGCAGC AGCAGCCTCT AGAAAAAAAA ATGAAGCCTT TTCCCATGGA GCCATATAAC 
CATAATCCCT CAGAAGTCAA GGTCCCAGAA TTCTACTGGG ATTCTTCCTA CAGCATGGCT 
GATAACAGAT CTGTAATGGC ACAGCAAGCA AACATAGACC GCAGGGGCAA ACGGTCACCA 
GGAGTCTTCC GTCCAGAGCA GGATCCTGTA CCCAGAATGC CGTTTGAGGA CCCCAAGAGC 
TCCCCTCTGC TTCCTCCGGA CCTGTTAAAG AGTCTGGCTG CCTTGGAGGA AGAGGAAGAG 
CTGATTTTTT CTAACCCTCC TGATCTTTAC CCGGCTCTGC TGGGGCCTCT CGCCTCTCTT 
CCTGGACGAA GCCTTTTTAA ATCCTTATTG GAGAAGCCCT CAGAGCTCAT GTCACATTCA 
TCCTCTTTCC TGTCCCTCAC CGGATTCTCT CTCAATCAGG AAAGATACCC AAATAATAGT 
ATGTTCAATG AGGTATATGG GAAAAACCTG ACATCCAGCT CCAAAGCAGA ACTCAGTCCC 
TCAATGGCCC CCCAGGAAAC ATCTCTGTAT TCCCTTTTTG AAGGGACTCC GTGGTCTCCA 
TCACTTCCTG CCAGTTCAGA TCATTCAACA CCAGCCAGCC AGTCTCCTCA TTCCTCTAAC 
CCAAGCAGCC TACCCAGCTC TCCTCCAACA CACAACCATA ATTCTGTTCC ATTCTCCAAT 
TTTGGACCCA TTGGGACTCC AGATAACAGG GATAGAAGGA CTGCAGATCG GTGGAAAACT 
GATAAGCCAG CCATGGGTGG GTTTGGCATT GATTATCTCT CAGCAACGTC ATCCTCTGAG 
AGCAGTTGGC ATCAGGCCAG CACTCCGAGT GGCACCTGGA CAGGCCATGG CCCTTCCATG 
GAGGATTCCT CTGCTGTCCT CATGGAAAGC CTAAAGTCTA TCTGGTCCAG TTCCATGATG 
CATCCTGGAC CTTCTGCTCT GGAGCAGCTG TTAATGCAGC AGAAGCAGAA ACAGCAACGG 
GGACAAGGCA CCATGAACCC TCCACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011508509.1
CDS	892..4401
Translation	MTDSKLGPAEVWTSRQALQDLYQKMLVTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRANPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQTHTSAIVKPQSSSCSYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNLQKALSKALESRDEVKTKWGVSDFIKAFIKFHGHVYLSKSLEKLSPLREKLEEQFKRLLFQKAFNSQQLVHVTVINLFQLHHLRDFSNETEQHTYSQDEQLCWTQLLALFMSFLGILCKCPLQNESQEESYNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPHEEDLSSISATPLPEEFELQGFLALRPSFRNLDFSKGHQGITGDKEGQQRRIRQQRLISIGKWIADNQPRLIQCENEVGKLLFITEIPELILEDPSEAKENLILQETSVIESLAADGSPGLKSVLSTSRNLSNNCDTGEKPVVTFKENIKTREVNRDQGRSFPPKEVRRDYSKGITVTKNDGKKDNNKRKTETKKCTLEKLQETGKQNVAVQVKSQTELRKTPVSEARKTPVTQTPTQASNSQFIPIHHPGAFPPLPSRPGFPPPTYVIPPPVAFSMGSGYTFPAGVSVPGTFLQPTAHSPAGNQVQAGKQSHIPYSQQRPSGPGPMNQGPQQSQPPSQQPLTSLPAQPTAQSTSQLQVQALTQQQQSPTKAVPALGKSPPHHSGFQQYQQADASKQLWNPPQVQGPLGKIMPVKQPYYLQTQDPIKLFEPSLQPPVMQQQPLEKKMKPFPMEPYNHNPSEVKVPEFYWDSSYSMADNRSVMAQQANIDRRGKRSPGVFRPEQDPVPRMPFEDPKSSPLLPPDLLKSLAALEEEEELIFSNPPDLYPALLGPLASLPGRSLFKSLLEKPSELMSHSSSFLSLTGFSLNQERYPNNSMFNEVYGKNLTSSSKAELSPSMAPQETSLYSLFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSVPFSNFGPIGTPDNRDRRTADRWKTDKPAMGGFGIDYLSATSSSESSWHQASTPSGTWTGHGPSMEDSSAVLMESLKSIWSSSMMHPGPSALEQLLMQQKQKQQRGQGTMNPPH

Target ORF information:

RefSeq Version	XM_011510207.2
Organism	Homo sapiens(human)
Definition	Homo sapiens SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011510207.2

ORF Insert Sequence:

ATGACAGATT CTAAGCTGGG TCCAGCTGAA GTCTGGACAT CCAGGCAGGC TCTGCAGGAC 
CTGTACCAGA AAATGCTAGT TACCGATTTG GAATACGCTT TAGACAAGAA AGTAGAACAG 
GATCTCTGGA ATCACGCCTT TAAGAATCAG ATCACAACAC TGCAAGGCCA GGCAAAGAAT 
CGAGCAAATC CGAATCGGAG TGAAGTTCAG GCAAACCTTT CTCTGTTCCT AGAGGCAGCT 
AGTGGCTTCT ATACTCAGTT ATTACAAGAA CTGTGTACAG TATTTAATGT AGATTTACCA 
TGCCGTGTGA AGTCTTCCCA ATTGGGAATT ATCAGCAATA AACAGACGCA TACCAGCGCC 
ATAGTGAAGC CACAGTCTAG CTCCTGTTCC TATATCTGCC AGCACTGCCT CGTCCACCTT 
GGAGACATTG CTCGATACAG AAACCAGACC AGCCAGGCAG AGTCCTACTA TAGGCATGCA 
GCTCAGCTTG TCCCCTCCAA TGGTCAGCCT TATAATCAGT TGGCTATCTT AGCTTCTTCC 
AAAGGAGACC ATCTGACCAC AATTTTCTAC TACTGCAGAA GCATTGCTGT GAAGTTCCCT 
TTCCCAGCTG CCTCCACTAA TCTGCAAAAA GCACTTTCTA AAGCACTGGA AAGCCGAGAT 
GAGGTGAAAA CCAAGTGGGG TGTTTCTGAC TTCATCAAGG CCTTTATTAA ATTCCACGGT 
CATGTGTACC TGAGTAAGAG CTTGGAAAAG TTGAGCCCTC TTCGAGAGAA ATTGGAAGAA 
CAGTTTAAGA GGCTGCTATT CCAAAAAGCT TTCAACTCTC AGCAGTTAGT TCATGTCACT 
GTCATTAACC TGTTTCAACT TCATCACCTT CGTGACTTTA GCAATGAAAC CGAGCAGCAC 
ACTTATAGCC AAGATGAGCA GCTATGTTGG ACACAGTTGC TGGCCCTCTT TATGTCTTTT 
CTTGGCATCC TGTGCAAGTG TCCTCTACAG AATGAGTCTC AGGAGGAGTC CTACAATGCC 
TATCCTCTTC CAGCAGTCAA GGTCTCCATG GACTGGCTAA GACTCAGACC CAGGGTCTTT 
CAGGAGGCAG TGGTGGATGA AAGACAGTAC ATTTGGCCCT GGTTGATTTC TCTTCTGAAT 
AGTTTCCATC CCCATGAAGA GGACCTCTCA AGTATTAGTG CGACACCACT TCCAGAGGAG 
TTTGAATTAC AAGGATTTTT GGCATTGAGA CCTTCTTTCA GGAACTTGGA TTTTTCCAAA 
GGTCACCAGG GTATTACAGG GGACAAAGAA GGCCAGCAAC GACGAATACG ACAGCAACGC 
TTGATCTCTA TAGGCAAATG GATTGCTGAT AATCAGCCAA GGCTGATTCA GTGTGAAAAT 
GAGGTAGGGA AATTGTTGTT TATCACAGAA ATCCCAGAAT TAATACTGGA AGACCCCAGT 
GAAGCCAAAG AGAACCTCAT TCTGCAAGAA ACATCTGTGA TAGAGTCGCT GGCTGCAGAT 
GGGAGCCCAG GGCTAAAATC AGTGCTATCT ACAAGCCGAA ATTTAAGCAA CAACTGTGAC 
ACAGGAGAGA AGCCAGTGGT TACCTTCAAA GAAAACATTA AGACACGAGA AGTGAACAGA 
GACCAAGGAA GAAGTTTTCC TCCCAAAGAG GTGAGAAGGG ACTATAGCAA AGGAATAACT 
GTAACTAAGA ATGATGGAAA GAAGGACAAC AACAAGAGGA AAACTGAAAC CAAGAAATGC 
ACCTTAGAAA AGTTACAGGA AACAGGAAAG CAGAATGTGG CAGTGCAGGT AAAATCCCAG 
ACAGAACTAA GAAAGACTCC AGTGTCTGAA GCCAGAAAAA CACCTGTAAC TCAAACCCCA 
ACTCAAGCAA GTAACTCCCA GTTCATCCCC ATTCATCACC CTGGAGCCTT CCCTCCTCTT 
CCCAGCAGGC CAGGGTTTCC GCCCCCAACA TATGTTATCC CCCCGCCTGT GGCATTTTCT 
ATGGGCTCAG GTTACACCTT CCCAGCTGGT GTTTCTGTCC CAGGAACCTT TCTTCAGCCT 
ACAGCTCACT CTCCAGCAGG AAACCAGGTG CAAGCTGGGA AACAGTCCCA CATTCCTTAC 
AGCCAGCAAC GGCCCTCTGG ACCAGGGCCA ATGAACCAGG GACCTCAACA ATCACAGCCA 
CCTTCCCAGC AACCCCTTAC ATCTTTACCA GCTCAGCCAA CAGCACAGTC TACAAGCCAG 
CTGCAGGTTC AAGCTCTAAC TCAGCAACAA CAATCCCCTA CAAAAGCTGT GCCGGCTTTG 
GGGAAAAGCC CGCCTCACCA CTCTGGATTC CAGCAGTATC AACAGGCAGA TGCCTCCAAA 
CAGCTGTGGA ATCCCCCTCA GGTTCAAGGC CCATTAGGGA AAATTATGCC TGTGAAACAG 
CCCTACTACC TTCAGACCCA AGACCCCATA AAACTGTTTG AGCCGTCATT GCAACCTCCT 
GTAATGCAGC AGCAGCCTCT AGAAAAAAAA ATGAAGCCTT TTCCCATGGA GCCATATAAC 
CATAATCCCT CAGAAGTCAA GGTCCCAGAA TTCTACTGGG ATTCTTCCTA CAGCATGGCT 
GATAACAGAT CTGTAATGGC ACAGCAAGCA AACATAGACC GCAGGGGCAA ACGGTCACCA 
GGAGTCTTCC GTCCAGAGCA GGATCCTGTA CCCAGAATGC CGTTTGAGGA CCCCAAGAGC 
TCCCCTCTGC TTCCTCCGGA CCTGTTAAAG AGTCTGGCTG CCTTGGAGGA AGAGGAAGAG 
CTGATTTTTT CTAACCCTCC TGATCTTTAC CCGGCTCTGC TGGGGCCTCT CGCCTCTCTT 
CCTGGACGAA GCCTTTTTAA ATCCTTATTG GAGAAGCCCT CAGAGCTCAT GTCACATTCA 
TCCTCTTTCC TGTCCCTCAC CGGATTCTCT CTCAATCAGG AAAGATACCC AAATAATAGT 
ATGTTCAATG AGGTATATGG GAAAAACCTG ACATCCAGCT CCAAAGCAGA ACTCAGTCCC 
TCAATGGCCC CCCAGGAAAC ATCTCTGTAT TCCCTTTTTG AAGGGACTCC GTGGTCTCCA 
TCACTTCCTG CCAGTTCAGA TCATTCAACA CCAGCCAGCC AGTCTCCTCA TTCCTCTAAC 
CCAAGCAGCC TACCCAGCTC TCCTCCAACA CACAACCATA ATTCTGTTCC ATTCTCCAAT 
TTTGGACCCA TTGGGACTCC AGATAACAGG GATAGAAGGA CTGCAGATCG GTGGAAAACT 
GATAAGCCAG CCATGGGTGG GTTTGGCATT GATTATCTCT CAGCAACGTC ATCCTCTGAG 
AGCAGTTGGC ATCAGGCCAG CACTCCGAGT GGCACCTGGA CAGGCCATGG CCCTTCCATG 
GAGGATTCCT CTGCTGTCCT CATGGAAAGC CTAAAGTCTA TCTGGTCCAG TTCCATGATG 
CATCCTGGAC CTTCTGCTCT GGAGCAGCTG TTAATGCAGC AGAAGCAGAA ACAGCAACGG 
GGACAAGGCA CCATGAACCC TCCACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG7 ( XM_011510207.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011508509.1 Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu34884
Clone ID Related Accession (Same CDS sequence)	XM_011510207.2 , XM_011510207.3 , XM_005245653.4 , XM_005245653.5
Accession Version	XM_011510207.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3510bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein SMG7 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011510207.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAGATT CTAAGCTGGG TCCAGCTGAA GTCTGGACAT CCAGGCAGGC TCTGCAGGAC 
CTGTACCAGA AAATGCTAGT TACCGATTTG GAATACGCTT TAGACAAGAA AGTAGAACAG 
GATCTCTGGA ATCACGCCTT TAAGAATCAG ATCACAACAC TGCAAGGCCA GGCAAAGAAT 
CGAGCAAATC CGAATCGGAG TGAAGTTCAG GCAAACCTTT CTCTGTTCCT AGAGGCAGCT 
AGTGGCTTCT ATACTCAGTT ATTACAAGAA CTGTGTACAG TATTTAATGT AGATTTACCA 
TGCCGTGTGA AGTCTTCCCA ATTGGGAATT ATCAGCAATA AACAGACGCA TACCAGCGCC 
ATAGTGAAGC CACAGTCTAG CTCCTGTTCC TATATCTGCC AGCACTGCCT CGTCCACCTT 
GGAGACATTG CTCGATACAG AAACCAGACC AGCCAGGCAG AGTCCTACTA TAGGCATGCA 
GCTCAGCTTG TCCCCTCCAA TGGTCAGCCT TATAATCAGT TGGCTATCTT AGCTTCTTCC 
AAAGGAGACC ATCTGACCAC AATTTTCTAC TACTGCAGAA GCATTGCTGT GAAGTTCCCT 
TTCCCAGCTG CCTCCACTAA TCTGCAAAAA GCACTTTCTA AAGCACTGGA AAGCCGAGAT 
GAGGTGAAAA CCAAGTGGGG TGTTTCTGAC TTCATCAAGG CCTTTATTAA ATTCCACGGT 
CATGTGTACC TGAGTAAGAG CTTGGAAAAG TTGAGCCCTC TTCGAGAGAA ATTGGAAGAA 
CAGTTTAAGA GGCTGCTATT CCAAAAAGCT TTCAACTCTC AGCAGTTAGT TCATGTCACT 
GTCATTAACC TGTTTCAACT TCATCACCTT CGTGACTTTA GCAATGAAAC CGAGCAGCAC 
ACTTATAGCC AAGATGAGCA GCTATGTTGG ACACAGTTGC TGGCCCTCTT TATGTCTTTT 
CTTGGCATCC TGTGCAAGTG TCCTCTACAG AATGAGTCTC AGGAGGAGTC CTACAATGCC 
TATCCTCTTC CAGCAGTCAA GGTCTCCATG GACTGGCTAA GACTCAGACC CAGGGTCTTT 
CAGGAGGCAG TGGTGGATGA AAGACAGTAC ATTTGGCCCT GGTTGATTTC TCTTCTGAAT 
AGTTTCCATC CCCATGAAGA GGACCTCTCA AGTATTAGTG CGACACCACT TCCAGAGGAG 
TTTGAATTAC AAGGATTTTT GGCATTGAGA CCTTCTTTCA GGAACTTGGA TTTTTCCAAA 
GGTCACCAGG GTATTACAGG GGACAAAGAA GGCCAGCAAC GACGAATACG ACAGCAACGC 
TTGATCTCTA TAGGCAAATG GATTGCTGAT AATCAGCCAA GGCTGATTCA GTGTGAAAAT 
GAGGTAGGGA AATTGTTGTT TATCACAGAA ATCCCAGAAT TAATACTGGA AGACCCCAGT 
GAAGCCAAAG AGAACCTCAT TCTGCAAGAA ACATCTGTGA TAGAGTCGCT GGCTGCAGAT 
GGGAGCCCAG GGCTAAAATC AGTGCTATCT ACAAGCCGAA ATTTAAGCAA CAACTGTGAC 
ACAGGAGAGA AGCCAGTGGT TACCTTCAAA GAAAACATTA AGACACGAGA AGTGAACAGA 
GACCAAGGAA GAAGTTTTCC TCCCAAAGAG GTGAGAAGGG ACTATAGCAA AGGAATAACT 
GTAACTAAGA ATGATGGAAA GAAGGACAAC AACAAGAGGA AAACTGAAAC CAAGAAATGC 
ACCTTAGAAA AGTTACAGGA AACAGGAAAG CAGAATGTGG CAGTGCAGGT AAAATCCCAG 
ACAGAACTAA GAAAGACTCC AGTGTCTGAA GCCAGAAAAA CACCTGTAAC TCAAACCCCA 
ACTCAAGCAA GTAACTCCCA GTTCATCCCC ATTCATCACC CTGGAGCCTT CCCTCCTCTT 
CCCAGCAGGC CAGGGTTTCC GCCCCCAACA TATGTTATCC CCCCGCCTGT GGCATTTTCT 
ATGGGCTCAG GTTACACCTT CCCAGCTGGT GTTTCTGTCC CAGGAACCTT TCTTCAGCCT 
ACAGCTCACT CTCCAGCAGG AAACCAGGTG CAAGCTGGGA AACAGTCCCA CATTCCTTAC 
AGCCAGCAAC GGCCCTCTGG ACCAGGGCCA ATGAACCAGG GACCTCAACA ATCACAGCCA 
CCTTCCCAGC AACCCCTTAC ATCTTTACCA GCTCAGCCAA CAGCACAGTC TACAAGCCAG 
CTGCAGGTTC AAGCTCTAAC TCAGCAACAA CAATCCCCTA CAAAAGCTGT GCCGGCTTTG 
GGGAAAAGCC CGCCTCACCA CTCTGGATTC CAGCAGTATC AACAGGCAGA TGCCTCCAAA 
CAGCTGTGGA ATCCCCCTCA GGTTCAAGGC CCATTAGGGA AAATTATGCC TGTGAAACAG 
CCCTACTACC TTCAGACCCA AGACCCCATA AAACTGTTTG AGCCGTCATT GCAACCTCCT 
GTAATGCAGC AGCAGCCTCT AGAAAAAAAA ATGAAGCCTT TTCCCATGGA GCCATATAAC 
CATAATCCCT CAGAAGTCAA GGTCCCAGAA TTCTACTGGG ATTCTTCCTA CAGCATGGCT 
GATAACAGAT CTGTAATGGC ACAGCAAGCA AACATAGACC GCAGGGGCAA ACGGTCACCA 
GGAGTCTTCC GTCCAGAGCA GGATCCTGTA CCCAGAATGC CGTTTGAGGA CCCCAAGAGC 
TCCCCTCTGC TTCCTCCGGA CCTGTTAAAG AGTCTGGCTG CCTTGGAGGA AGAGGAAGAG 
CTGATTTTTT CTAACCCTCC TGATCTTTAC CCGGCTCTGC TGGGGCCTCT CGCCTCTCTT 
CCTGGACGAA GCCTTTTTAA ATCCTTATTG GAGAAGCCCT CAGAGCTCAT GTCACATTCA 
TCCTCTTTCC TGTCCCTCAC CGGATTCTCT CTCAATCAGG AAAGATACCC AAATAATAGT 
ATGTTCAATG AGGTATATGG GAAAAACCTG ACATCCAGCT CCAAAGCAGA ACTCAGTCCC 
TCAATGGCCC CCCAGGAAAC ATCTCTGTAT TCCCTTTTTG AAGGGACTCC GTGGTCTCCA 
TCACTTCCTG CCAGTTCAGA TCATTCAACA CCAGCCAGCC AGTCTCCTCA TTCCTCTAAC 
CCAAGCAGCC TACCCAGCTC TCCTCCAACA CACAACCATA ATTCTGTTCC ATTCTCCAAT 
TTTGGACCCA TTGGGACTCC AGATAACAGG GATAGAAGGA CTGCAGATCG GTGGAAAACT 
GATAAGCCAG CCATGGGTGG GTTTGGCATT GATTATCTCT CAGCAACGTC ATCCTCTGAG 
AGCAGTTGGC ATCAGGCCAG CACTCCGAGT GGCACCTGGA CAGGCCATGG CCCTTCCATG 
GAGGATTCCT CTGCTGTCCT CATGGAAAGC CTAAAGTCTA TCTGGTCCAG TTCCATGATG 
CATCCTGGAC CTTCTGCTCT GGAGCAGCTG TTAATGCAGC AGAAGCAGAA ACAGCAACGG 
GGACAAGGCA CCATGAACCC TCCACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011508509.1
CDS	861..4370
Translation	MTDSKLGPAEVWTSRQALQDLYQKMLVTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRANPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQTHTSAIVKPQSSSCSYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNLQKALSKALESRDEVKTKWGVSDFIKAFIKFHGHVYLSKSLEKLSPLREKLEEQFKRLLFQKAFNSQQLVHVTVINLFQLHHLRDFSNETEQHTYSQDEQLCWTQLLALFMSFLGILCKCPLQNESQEESYNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPHEEDLSSISATPLPEEFELQGFLALRPSFRNLDFSKGHQGITGDKEGQQRRIRQQRLISIGKWIADNQPRLIQCENEVGKLLFITEIPELILEDPSEAKENLILQETSVIESLAADGSPGLKSVLSTSRNLSNNCDTGEKPVVTFKENIKTREVNRDQGRSFPPKEVRRDYSKGITVTKNDGKKDNNKRKTETKKCTLEKLQETGKQNVAVQVKSQTELRKTPVSEARKTPVTQTPTQASNSQFIPIHHPGAFPPLPSRPGFPPPTYVIPPPVAFSMGSGYTFPAGVSVPGTFLQPTAHSPAGNQVQAGKQSHIPYSQQRPSGPGPMNQGPQQSQPPSQQPLTSLPAQPTAQSTSQLQVQALTQQQQSPTKAVPALGKSPPHHSGFQQYQQADASKQLWNPPQVQGPLGKIMPVKQPYYLQTQDPIKLFEPSLQPPVMQQQPLEKKMKPFPMEPYNHNPSEVKVPEFYWDSSYSMADNRSVMAQQANIDRRGKRSPGVFRPEQDPVPRMPFEDPKSSPLLPPDLLKSLAALEEEEELIFSNPPDLYPALLGPLASLPGRSLFKSLLEKPSELMSHSSSFLSLTGFSLNQERYPNNSMFNEVYGKNLTSSSKAELSPSMAPQETSLYSLFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSVPFSNFGPIGTPDNRDRRTADRWKTDKPAMGGFGIDYLSATSSSESSWHQASTPSGTWTGHGPSMEDSSAVLMESLKSIWSSSMMHPGPSALEQLLMQQKQKQQRGQGTMNPPH

Target ORF information:

RefSeq Version	XM_011510207.3
Organism	Homo sapiens(human)
Definition	Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011510207.3

ORF Insert Sequence:

ATGACAGATT CTAAGCTGGG TCCAGCTGAA GTCTGGACAT CCAGGCAGGC TCTGCAGGAC 
CTGTACCAGA AAATGCTAGT TACCGATTTG GAATACGCTT TAGACAAGAA AGTAGAACAG 
GATCTCTGGA ATCACGCCTT TAAGAATCAG ATCACAACAC TGCAAGGCCA GGCAAAGAAT 
CGAGCAAATC CGAATCGGAG TGAAGTTCAG GCAAACCTTT CTCTGTTCCT AGAGGCAGCT 
AGTGGCTTCT ATACTCAGTT ATTACAAGAA CTGTGTACAG TATTTAATGT AGATTTACCA 
TGCCGTGTGA AGTCTTCCCA ATTGGGAATT ATCAGCAATA AACAGACGCA TACCAGCGCC 
ATAGTGAAGC CACAGTCTAG CTCCTGTTCC TATATCTGCC AGCACTGCCT CGTCCACCTT 
GGAGACATTG CTCGATACAG AAACCAGACC AGCCAGGCAG AGTCCTACTA TAGGCATGCA 
GCTCAGCTTG TCCCCTCCAA TGGTCAGCCT TATAATCAGT TGGCTATCTT AGCTTCTTCC 
AAAGGAGACC ATCTGACCAC AATTTTCTAC TACTGCAGAA GCATTGCTGT GAAGTTCCCT 
TTCCCAGCTG CCTCCACTAA TCTGCAAAAA GCACTTTCTA AAGCACTGGA AAGCCGAGAT 
GAGGTGAAAA CCAAGTGGGG TGTTTCTGAC TTCATCAAGG CCTTTATTAA ATTCCACGGT 
CATGTGTACC TGAGTAAGAG CTTGGAAAAG TTGAGCCCTC TTCGAGAGAA ATTGGAAGAA 
CAGTTTAAGA GGCTGCTATT CCAAAAAGCT TTCAACTCTC AGCAGTTAGT TCATGTCACT 
GTCATTAACC TGTTTCAACT TCATCACCTT CGTGACTTTA GCAATGAAAC CGAGCAGCAC 
ACTTATAGCC AAGATGAGCA GCTATGTTGG ACACAGTTGC TGGCCCTCTT TATGTCTTTT 
CTTGGCATCC TGTGCAAGTG TCCTCTACAG AATGAGTCTC AGGAGGAGTC CTACAATGCC 
TATCCTCTTC CAGCAGTCAA GGTCTCCATG GACTGGCTAA GACTCAGACC CAGGGTCTTT 
CAGGAGGCAG TGGTGGATGA AAGACAGTAC ATTTGGCCCT GGTTGATTTC TCTTCTGAAT 
AGTTTCCATC CCCATGAAGA GGACCTCTCA AGTATTAGTG CGACACCACT TCCAGAGGAG 
TTTGAATTAC AAGGATTTTT GGCATTGAGA CCTTCTTTCA GGAACTTGGA TTTTTCCAAA 
GGTCACCAGG GTATTACAGG GGACAAAGAA GGCCAGCAAC GACGAATACG ACAGCAACGC 
TTGATCTCTA TAGGCAAATG GATTGCTGAT AATCAGCCAA GGCTGATTCA GTGTGAAAAT 
GAGGTAGGGA AATTGTTGTT TATCACAGAA ATCCCAGAAT TAATACTGGA AGACCCCAGT 
GAAGCCAAAG AGAACCTCAT TCTGCAAGAA ACATCTGTGA TAGAGTCGCT GGCTGCAGAT 
GGGAGCCCAG GGCTAAAATC AGTGCTATCT ACAAGCCGAA ATTTAAGCAA CAACTGTGAC 
ACAGGAGAGA AGCCAGTGGT TACCTTCAAA GAAAACATTA AGACACGAGA AGTGAACAGA 
GACCAAGGAA GAAGTTTTCC TCCCAAAGAG GTGAGAAGGG ACTATAGCAA AGGAATAACT 
GTAACTAAGA ATGATGGAAA GAAGGACAAC AACAAGAGGA AAACTGAAAC CAAGAAATGC 
ACCTTAGAAA AGTTACAGGA AACAGGAAAG CAGAATGTGG CAGTGCAGGT AAAATCCCAG 
ACAGAACTAA GAAAGACTCC AGTGTCTGAA GCCAGAAAAA CACCTGTAAC TCAAACCCCA 
ACTCAAGCAA GTAACTCCCA GTTCATCCCC ATTCATCACC CTGGAGCCTT CCCTCCTCTT 
CCCAGCAGGC CAGGGTTTCC GCCCCCAACA TATGTTATCC CCCCGCCTGT GGCATTTTCT 
ATGGGCTCAG GTTACACCTT CCCAGCTGGT GTTTCTGTCC CAGGAACCTT TCTTCAGCCT 
ACAGCTCACT CTCCAGCAGG AAACCAGGTG CAAGCTGGGA AACAGTCCCA CATTCCTTAC 
AGCCAGCAAC GGCCCTCTGG ACCAGGGCCA ATGAACCAGG GACCTCAACA ATCACAGCCA 
CCTTCCCAGC AACCCCTTAC ATCTTTACCA GCTCAGCCAA CAGCACAGTC TACAAGCCAG 
CTGCAGGTTC AAGCTCTAAC TCAGCAACAA CAATCCCCTA CAAAAGCTGT GCCGGCTTTG 
GGGAAAAGCC CGCCTCACCA CTCTGGATTC CAGCAGTATC AACAGGCAGA TGCCTCCAAA 
CAGCTGTGGA ATCCCCCTCA GGTTCAAGGC CCATTAGGGA AAATTATGCC TGTGAAACAG 
CCCTACTACC TTCAGACCCA AGACCCCATA AAACTGTTTG AGCCGTCATT GCAACCTCCT 
GTAATGCAGC AGCAGCCTCT AGAAAAAAAA ATGAAGCCTT TTCCCATGGA GCCATATAAC 
CATAATCCCT CAGAAGTCAA GGTCCCAGAA TTCTACTGGG ATTCTTCCTA CAGCATGGCT 
GATAACAGAT CTGTAATGGC ACAGCAAGCA AACATAGACC GCAGGGGCAA ACGGTCACCA 
GGAGTCTTCC GTCCAGAGCA GGATCCTGTA CCCAGAATGC CGTTTGAGGA CCCCAAGAGC 
TCCCCTCTGC TTCCTCCGGA CCTGTTAAAG AGTCTGGCTG CCTTGGAGGA AGAGGAAGAG 
CTGATTTTTT CTAACCCTCC TGATCTTTAC CCGGCTCTGC TGGGGCCTCT CGCCTCTCTT 
CCTGGACGAA GCCTTTTTAA ATCCTTATTG GAGAAGCCCT CAGAGCTCAT GTCACATTCA 
TCCTCTTTCC TGTCCCTCAC CGGATTCTCT CTCAATCAGG AAAGATACCC AAATAATAGT 
ATGTTCAATG AGGTATATGG GAAAAACCTG ACATCCAGCT CCAAAGCAGA ACTCAGTCCC 
TCAATGGCCC CCCAGGAAAC ATCTCTGTAT TCCCTTTTTG AAGGGACTCC GTGGTCTCCA 
TCACTTCCTG CCAGTTCAGA TCATTCAACA CCAGCCAGCC AGTCTCCTCA TTCCTCTAAC 
CCAAGCAGCC TACCCAGCTC TCCTCCAACA CACAACCATA ATTCTGTTCC ATTCTCCAAT 
TTTGGACCCA TTGGGACTCC AGATAACAGG GATAGAAGGA CTGCAGATCG GTGGAAAACT 
GATAAGCCAG CCATGGGTGG GTTTGGCATT GATTATCTCT CAGCAACGTC ATCCTCTGAG 
AGCAGTTGGC ATCAGGCCAG CACTCCGAGT GGCACCTGGA CAGGCCATGG CCCTTCCATG 
GAGGATTCCT CTGCTGTCCT CATGGAAAGC CTAAAGTCTA TCTGGTCCAG TTCCATGATG 
CATCCTGGAC CTTCTGCTCT GGAGCAGCTG TTAATGCAGC AGAAGCAGAA ACAGCAACGG 
GGACAAGGCA CCATGAACCC TCCACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG7 ( XM_005245653.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005245710.1 Homo sapiens SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X6, mRNA.

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CloneID	OHu34884
Clone ID Related Accession (Same CDS sequence)	XM_011510207.2 , XM_011510207.3 , XM_005245653.4 , XM_005245653.5
Accession Version	XM_005245653.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3510bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	protein SMG7 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005245653.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAGATT CTAAGCTGGG TCCAGCTGAA GTCTGGACAT CCAGGCAGGC TCTGCAGGAC 
CTGTACCAGA AAATGCTAGT TACCGATTTG GAATACGCTT TAGACAAGAA AGTAGAACAG 
GATCTCTGGA ATCACGCCTT TAAGAATCAG ATCACAACAC TGCAAGGCCA GGCAAAGAAT 
CGAGCAAATC CGAATCGGAG TGAAGTTCAG GCAAACCTTT CTCTGTTCCT AGAGGCAGCT 
AGTGGCTTCT ATACTCAGTT ATTACAAGAA CTGTGTACAG TATTTAATGT AGATTTACCA 
TGCCGTGTGA AGTCTTCCCA ATTGGGAATT ATCAGCAATA AACAGACGCA TACCAGCGCC 
ATAGTGAAGC CACAGTCTAG CTCCTGTTCC TATATCTGCC AGCACTGCCT CGTCCACCTT 
GGAGACATTG CTCGATACAG AAACCAGACC AGCCAGGCAG AGTCCTACTA TAGGCATGCA 
GCTCAGCTTG TCCCCTCCAA TGGTCAGCCT TATAATCAGT TGGCTATCTT AGCTTCTTCC 
AAAGGAGACC ATCTGACCAC AATTTTCTAC TACTGCAGAA GCATTGCTGT GAAGTTCCCT 
TTCCCAGCTG CCTCCACTAA TCTGCAAAAA GCACTTTCTA AAGCACTGGA AAGCCGAGAT 
GAGGTGAAAA CCAAGTGGGG TGTTTCTGAC TTCATCAAGG CCTTTATTAA ATTCCACGGT 
CATGTGTACC TGAGTAAGAG CTTGGAAAAG TTGAGCCCTC TTCGAGAGAA ATTGGAAGAA 
CAGTTTAAGA GGCTGCTATT CCAAAAAGCT TTCAACTCTC AGCAGTTAGT TCATGTCACT 
GTCATTAACC TGTTTCAACT TCATCACCTT CGTGACTTTA GCAATGAAAC CGAGCAGCAC 
ACTTATAGCC AAGATGAGCA GCTATGTTGG ACACAGTTGC TGGCCCTCTT TATGTCTTTT 
CTTGGCATCC TGTGCAAGTG TCCTCTACAG AATGAGTCTC AGGAGGAGTC CTACAATGCC 
TATCCTCTTC CAGCAGTCAA GGTCTCCATG GACTGGCTAA GACTCAGACC CAGGGTCTTT 
CAGGAGGCAG TGGTGGATGA AAGACAGTAC ATTTGGCCCT GGTTGATTTC TCTTCTGAAT 
AGTTTCCATC CCCATGAAGA GGACCTCTCA AGTATTAGTG CGACACCACT TCCAGAGGAG 
TTTGAATTAC AAGGATTTTT GGCATTGAGA CCTTCTTTCA GGAACTTGGA TTTTTCCAAA 
GGTCACCAGG GTATTACAGG GGACAAAGAA GGCCAGCAAC GACGAATACG ACAGCAACGC 
TTGATCTCTA TAGGCAAATG GATTGCTGAT AATCAGCCAA GGCTGATTCA GTGTGAAAAT 
GAGGTAGGGA AATTGTTGTT TATCACAGAA ATCCCAGAAT TAATACTGGA AGACCCCAGT 
GAAGCCAAAG AGAACCTCAT TCTGCAAGAA ACATCTGTGA TAGAGTCGCT GGCTGCAGAT 
GGGAGCCCAG GGCTAAAATC AGTGCTATCT ACAAGCCGAA ATTTAAGCAA CAACTGTGAC 
ACAGGAGAGA AGCCAGTGGT TACCTTCAAA GAAAACATTA AGACACGAGA AGTGAACAGA 
GACCAAGGAA GAAGTTTTCC TCCCAAAGAG GTGAGAAGGG ACTATAGCAA AGGAATAACT 
GTAACTAAGA ATGATGGAAA GAAGGACAAC AACAAGAGGA AAACTGAAAC CAAGAAATGC 
ACCTTAGAAA AGTTACAGGA AACAGGAAAG CAGAATGTGG CAGTGCAGGT AAAATCCCAG 
ACAGAACTAA GAAAGACTCC AGTGTCTGAA GCCAGAAAAA CACCTGTAAC TCAAACCCCA 
ACTCAAGCAA GTAACTCCCA GTTCATCCCC ATTCATCACC CTGGAGCCTT CCCTCCTCTT 
CCCAGCAGGC CAGGGTTTCC GCCCCCAACA TATGTTATCC CCCCGCCTGT GGCATTTTCT 
ATGGGCTCAG GTTACACCTT CCCAGCTGGT GTTTCTGTCC CAGGAACCTT TCTTCAGCCT 
ACAGCTCACT CTCCAGCAGG AAACCAGGTG CAAGCTGGGA AACAGTCCCA CATTCCTTAC 
AGCCAGCAAC GGCCCTCTGG ACCAGGGCCA ATGAACCAGG GACCTCAACA ATCACAGCCA 
CCTTCCCAGC AACCCCTTAC ATCTTTACCA GCTCAGCCAA CAGCACAGTC TACAAGCCAG 
CTGCAGGTTC AAGCTCTAAC TCAGCAACAA CAATCCCCTA CAAAAGCTGT GCCGGCTTTG 
GGGAAAAGCC CGCCTCACCA CTCTGGATTC CAGCAGTATC AACAGGCAGA TGCCTCCAAA 
CAGCTGTGGA ATCCCCCTCA GGTTCAAGGC CCATTAGGGA AAATTATGCC TGTGAAACAG 
CCCTACTACC TTCAGACCCA AGACCCCATA AAACTGTTTG AGCCGTCATT GCAACCTCCT 
GTAATGCAGC AGCAGCCTCT AGAAAAAAAA ATGAAGCCTT TTCCCATGGA GCCATATAAC 
CATAATCCCT CAGAAGTCAA GGTCCCAGAA TTCTACTGGG ATTCTTCCTA CAGCATGGCT 
GATAACAGAT CTGTAATGGC ACAGCAAGCA AACATAGACC GCAGGGGCAA ACGGTCACCA 
GGAGTCTTCC GTCCAGAGCA GGATCCTGTA CCCAGAATGC CGTTTGAGGA CCCCAAGAGC 
TCCCCTCTGC TTCCTCCGGA CCTGTTAAAG AGTCTGGCTG CCTTGGAGGA AGAGGAAGAG 
CTGATTTTTT CTAACCCTCC TGATCTTTAC CCGGCTCTGC TGGGGCCTCT CGCCTCTCTT 
CCTGGACGAA GCCTTTTTAA ATCCTTATTG GAGAAGCCCT CAGAGCTCAT GTCACATTCA 
TCCTCTTTCC TGTCCCTCAC CGGATTCTCT CTCAATCAGG AAAGATACCC AAATAATAGT 
ATGTTCAATG AGGTATATGG GAAAAACCTG ACATCCAGCT CCAAAGCAGA ACTCAGTCCC 
TCAATGGCCC CCCAGGAAAC ATCTCTGTAT TCCCTTTTTG AAGGGACTCC GTGGTCTCCA 
TCACTTCCTG CCAGTTCAGA TCATTCAACA CCAGCCAGCC AGTCTCCTCA TTCCTCTAAC 
CCAAGCAGCC TACCCAGCTC TCCTCCAACA CACAACCATA ATTCTGTTCC ATTCTCCAAT 
TTTGGACCCA TTGGGACTCC AGATAACAGG GATAGAAGGA CTGCAGATCG GTGGAAAACT 
GATAAGCCAG CCATGGGTGG GTTTGGCATT GATTATCTCT CAGCAACGTC ATCCTCTGAG 
AGCAGTTGGC ATCAGGCCAG CACTCCGAGT GGCACCTGGA CAGGCCATGG CCCTTCCATG 
GAGGATTCCT CTGCTGTCCT CATGGAAAGC CTAAAGTCTA TCTGGTCCAG TTCCATGATG 
CATCCTGGAC CTTCTGCTCT GGAGCAGCTG TTAATGCAGC AGAAGCAGAA ACAGCAACGG 
GGACAAGGCA CCATGAACCC TCCACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005245710.1
CDS	276..3785
Translation	MTDSKLGPAEVWTSRQALQDLYQKMLVTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRANPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQTHTSAIVKPQSSSCSYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNLQKALSKALESRDEVKTKWGVSDFIKAFIKFHGHVYLSKSLEKLSPLREKLEEQFKRLLFQKAFNSQQLVHVTVINLFQLHHLRDFSNETEQHTYSQDEQLCWTQLLALFMSFLGILCKCPLQNESQEESYNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPHEEDLSSISATPLPEEFELQGFLALRPSFRNLDFSKGHQGITGDKEGQQRRIRQQRLISIGKWIADNQPRLIQCENEVGKLLFITEIPELILEDPSEAKENLILQETSVIESLAADGSPGLKSVLSTSRNLSNNCDTGEKPVVTFKENIKTREVNRDQGRSFPPKEVRRDYSKGITVTKNDGKKDNNKRKTETKKCTLEKLQETGKQNVAVQVKSQTELRKTPVSEARKTPVTQTPTQASNSQFIPIHHPGAFPPLPSRPGFPPPTYVIPPPVAFSMGSGYTFPAGVSVPGTFLQPTAHSPAGNQVQAGKQSHIPYSQQRPSGPGPMNQGPQQSQPPSQQPLTSLPAQPTAQSTSQLQVQALTQQQQSPTKAVPALGKSPPHHSGFQQYQQADASKQLWNPPQVQGPLGKIMPVKQPYYLQTQDPIKLFEPSLQPPVMQQQPLEKKMKPFPMEPYNHNPSEVKVPEFYWDSSYSMADNRSVMAQQANIDRRGKRSPGVFRPEQDPVPRMPFEDPKSSPLLPPDLLKSLAALEEEEELIFSNPPDLYPALLGPLASLPGRSLFKSLLEKPSELMSHSSSFLSLTGFSLNQERYPNNSMFNEVYGKNLTSSSKAELSPSMAPQETSLYSLFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSVPFSNFGPIGTPDNRDRRTADRWKTDKPAMGGFGIDYLSATSSSESSWHQASTPSGTWTGHGPSMEDSSAVLMESLKSIWSSSMMHPGPSALEQLLMQQKQKQQRGQGTMNPPH

Target ORF information:

RefSeq Version	XM_005245653.4
Organism	Homo sapiens(human)
Definition	Homo sapiens SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005245653.4

ORF Insert Sequence:

ATGACAGATT CTAAGCTGGG TCCAGCTGAA GTCTGGACAT CCAGGCAGGC TCTGCAGGAC 
CTGTACCAGA AAATGCTAGT TACCGATTTG GAATACGCTT TAGACAAGAA AGTAGAACAG 
GATCTCTGGA ATCACGCCTT TAAGAATCAG ATCACAACAC TGCAAGGCCA GGCAAAGAAT 
CGAGCAAATC CGAATCGGAG TGAAGTTCAG GCAAACCTTT CTCTGTTCCT AGAGGCAGCT 
AGTGGCTTCT ATACTCAGTT ATTACAAGAA CTGTGTACAG TATTTAATGT AGATTTACCA 
TGCCGTGTGA AGTCTTCCCA ATTGGGAATT ATCAGCAATA AACAGACGCA TACCAGCGCC 
ATAGTGAAGC CACAGTCTAG CTCCTGTTCC TATATCTGCC AGCACTGCCT CGTCCACCTT 
GGAGACATTG CTCGATACAG AAACCAGACC AGCCAGGCAG AGTCCTACTA TAGGCATGCA 
GCTCAGCTTG TCCCCTCCAA TGGTCAGCCT TATAATCAGT TGGCTATCTT AGCTTCTTCC 
AAAGGAGACC ATCTGACCAC AATTTTCTAC TACTGCAGAA GCATTGCTGT GAAGTTCCCT 
TTCCCAGCTG CCTCCACTAA TCTGCAAAAA GCACTTTCTA AAGCACTGGA AAGCCGAGAT 
GAGGTGAAAA CCAAGTGGGG TGTTTCTGAC TTCATCAAGG CCTTTATTAA ATTCCACGGT 
CATGTGTACC TGAGTAAGAG CTTGGAAAAG TTGAGCCCTC TTCGAGAGAA ATTGGAAGAA 
CAGTTTAAGA GGCTGCTATT CCAAAAAGCT TTCAACTCTC AGCAGTTAGT TCATGTCACT 
GTCATTAACC TGTTTCAACT TCATCACCTT CGTGACTTTA GCAATGAAAC CGAGCAGCAC 
ACTTATAGCC AAGATGAGCA GCTATGTTGG ACACAGTTGC TGGCCCTCTT TATGTCTTTT 
CTTGGCATCC TGTGCAAGTG TCCTCTACAG AATGAGTCTC AGGAGGAGTC CTACAATGCC 
TATCCTCTTC CAGCAGTCAA GGTCTCCATG GACTGGCTAA GACTCAGACC CAGGGTCTTT 
CAGGAGGCAG TGGTGGATGA AAGACAGTAC ATTTGGCCCT GGTTGATTTC TCTTCTGAAT 
AGTTTCCATC CCCATGAAGA GGACCTCTCA AGTATTAGTG CGACACCACT TCCAGAGGAG 
TTTGAATTAC AAGGATTTTT GGCATTGAGA CCTTCTTTCA GGAACTTGGA TTTTTCCAAA 
GGTCACCAGG GTATTACAGG GGACAAAGAA GGCCAGCAAC GACGAATACG ACAGCAACGC 
TTGATCTCTA TAGGCAAATG GATTGCTGAT AATCAGCCAA GGCTGATTCA GTGTGAAAAT 
GAGGTAGGGA AATTGTTGTT TATCACAGAA ATCCCAGAAT TAATACTGGA AGACCCCAGT 
GAAGCCAAAG AGAACCTCAT TCTGCAAGAA ACATCTGTGA TAGAGTCGCT GGCTGCAGAT 
GGGAGCCCAG GGCTAAAATC AGTGCTATCT ACAAGCCGAA ATTTAAGCAA CAACTGTGAC 
ACAGGAGAGA AGCCAGTGGT TACCTTCAAA GAAAACATTA AGACACGAGA AGTGAACAGA 
GACCAAGGAA GAAGTTTTCC TCCCAAAGAG GTGAGAAGGG ACTATAGCAA AGGAATAACT 
GTAACTAAGA ATGATGGAAA GAAGGACAAC AACAAGAGGA AAACTGAAAC CAAGAAATGC 
ACCTTAGAAA AGTTACAGGA AACAGGAAAG CAGAATGTGG CAGTGCAGGT AAAATCCCAG 
ACAGAACTAA GAAAGACTCC AGTGTCTGAA GCCAGAAAAA CACCTGTAAC TCAAACCCCA 
ACTCAAGCAA GTAACTCCCA GTTCATCCCC ATTCATCACC CTGGAGCCTT CCCTCCTCTT 
CCCAGCAGGC CAGGGTTTCC GCCCCCAACA TATGTTATCC CCCCGCCTGT GGCATTTTCT 
ATGGGCTCAG GTTACACCTT CCCAGCTGGT GTTTCTGTCC CAGGAACCTT TCTTCAGCCT 
ACAGCTCACT CTCCAGCAGG AAACCAGGTG CAAGCTGGGA AACAGTCCCA CATTCCTTAC 
AGCCAGCAAC GGCCCTCTGG ACCAGGGCCA ATGAACCAGG GACCTCAACA ATCACAGCCA 
CCTTCCCAGC AACCCCTTAC ATCTTTACCA GCTCAGCCAA CAGCACAGTC TACAAGCCAG 
CTGCAGGTTC AAGCTCTAAC TCAGCAACAA CAATCCCCTA CAAAAGCTGT GCCGGCTTTG 
GGGAAAAGCC CGCCTCACCA CTCTGGATTC CAGCAGTATC AACAGGCAGA TGCCTCCAAA 
CAGCTGTGGA ATCCCCCTCA GGTTCAAGGC CCATTAGGGA AAATTATGCC TGTGAAACAG 
CCCTACTACC TTCAGACCCA AGACCCCATA AAACTGTTTG AGCCGTCATT GCAACCTCCT 
GTAATGCAGC AGCAGCCTCT AGAAAAAAAA ATGAAGCCTT TTCCCATGGA GCCATATAAC 
CATAATCCCT CAGAAGTCAA GGTCCCAGAA TTCTACTGGG ATTCTTCCTA CAGCATGGCT 
GATAACAGAT CTGTAATGGC ACAGCAAGCA AACATAGACC GCAGGGGCAA ACGGTCACCA 
GGAGTCTTCC GTCCAGAGCA GGATCCTGTA CCCAGAATGC CGTTTGAGGA CCCCAAGAGC 
TCCCCTCTGC TTCCTCCGGA CCTGTTAAAG AGTCTGGCTG CCTTGGAGGA AGAGGAAGAG 
CTGATTTTTT CTAACCCTCC TGATCTTTAC CCGGCTCTGC TGGGGCCTCT CGCCTCTCTT 
CCTGGACGAA GCCTTTTTAA ATCCTTATTG GAGAAGCCCT CAGAGCTCAT GTCACATTCA 
TCCTCTTTCC TGTCCCTCAC CGGATTCTCT CTCAATCAGG AAAGATACCC AAATAATAGT 
ATGTTCAATG AGGTATATGG GAAAAACCTG ACATCCAGCT CCAAAGCAGA ACTCAGTCCC 
TCAATGGCCC CCCAGGAAAC ATCTCTGTAT TCCCTTTTTG AAGGGACTCC GTGGTCTCCA 
TCACTTCCTG CCAGTTCAGA TCATTCAACA CCAGCCAGCC AGTCTCCTCA TTCCTCTAAC 
CCAAGCAGCC TACCCAGCTC TCCTCCAACA CACAACCATA ATTCTGTTCC ATTCTCCAAT 
TTTGGACCCA TTGGGACTCC AGATAACAGG GATAGAAGGA CTGCAGATCG GTGGAAAACT 
GATAAGCCAG CCATGGGTGG GTTTGGCATT GATTATCTCT CAGCAACGTC ATCCTCTGAG 
AGCAGTTGGC ATCAGGCCAG CACTCCGAGT GGCACCTGGA CAGGCCATGG CCCTTCCATG 
GAGGATTCCT CTGCTGTCCT CATGGAAAGC CTAAAGTCTA TCTGGTCCAG TTCCATGATG 
CATCCTGGAC CTTCTGCTCT GGAGCAGCTG TTAATGCAGC AGAAGCAGAA ACAGCAACGG 
GGACAAGGCA CCATGAACCC TCCACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG7 ( XM_005245653.5 ) cDNA ORF clone, Homo sapiens(human) -> XP_005245710.1 Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X6, mRNA.

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CloneID	OHu34884
Clone ID Related Accession (Same CDS sequence)	XM_011510207.2 , XM_011510207.3 , XM_005245653.4 , XM_005245653.5
Accession Version	XM_005245653.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3510bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein SMG7 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005245653.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAGATT CTAAGCTGGG TCCAGCTGAA GTCTGGACAT CCAGGCAGGC TCTGCAGGAC 
CTGTACCAGA AAATGCTAGT TACCGATTTG GAATACGCTT TAGACAAGAA AGTAGAACAG 
GATCTCTGGA ATCACGCCTT TAAGAATCAG ATCACAACAC TGCAAGGCCA GGCAAAGAAT 
CGAGCAAATC CGAATCGGAG TGAAGTTCAG GCAAACCTTT CTCTGTTCCT AGAGGCAGCT 
AGTGGCTTCT ATACTCAGTT ATTACAAGAA CTGTGTACAG TATTTAATGT AGATTTACCA 
TGCCGTGTGA AGTCTTCCCA ATTGGGAATT ATCAGCAATA AACAGACGCA TACCAGCGCC 
ATAGTGAAGC CACAGTCTAG CTCCTGTTCC TATATCTGCC AGCACTGCCT CGTCCACCTT 
GGAGACATTG CTCGATACAG AAACCAGACC AGCCAGGCAG AGTCCTACTA TAGGCATGCA 
GCTCAGCTTG TCCCCTCCAA TGGTCAGCCT TATAATCAGT TGGCTATCTT AGCTTCTTCC 
AAAGGAGACC ATCTGACCAC AATTTTCTAC TACTGCAGAA GCATTGCTGT GAAGTTCCCT 
TTCCCAGCTG CCTCCACTAA TCTGCAAAAA GCACTTTCTA AAGCACTGGA AAGCCGAGAT 
GAGGTGAAAA CCAAGTGGGG TGTTTCTGAC TTCATCAAGG CCTTTATTAA ATTCCACGGT 
CATGTGTACC TGAGTAAGAG CTTGGAAAAG TTGAGCCCTC TTCGAGAGAA ATTGGAAGAA 
CAGTTTAAGA GGCTGCTATT CCAAAAAGCT TTCAACTCTC AGCAGTTAGT TCATGTCACT 
GTCATTAACC TGTTTCAACT TCATCACCTT CGTGACTTTA GCAATGAAAC CGAGCAGCAC 
ACTTATAGCC AAGATGAGCA GCTATGTTGG ACACAGTTGC TGGCCCTCTT TATGTCTTTT 
CTTGGCATCC TGTGCAAGTG TCCTCTACAG AATGAGTCTC AGGAGGAGTC CTACAATGCC 
TATCCTCTTC CAGCAGTCAA GGTCTCCATG GACTGGCTAA GACTCAGACC CAGGGTCTTT 
CAGGAGGCAG TGGTGGATGA AAGACAGTAC ATTTGGCCCT GGTTGATTTC TCTTCTGAAT 
AGTTTCCATC CCCATGAAGA GGACCTCTCA AGTATTAGTG CGACACCACT TCCAGAGGAG 
TTTGAATTAC AAGGATTTTT GGCATTGAGA CCTTCTTTCA GGAACTTGGA TTTTTCCAAA 
GGTCACCAGG GTATTACAGG GGACAAAGAA GGCCAGCAAC GACGAATACG ACAGCAACGC 
TTGATCTCTA TAGGCAAATG GATTGCTGAT AATCAGCCAA GGCTGATTCA GTGTGAAAAT 
GAGGTAGGGA AATTGTTGTT TATCACAGAA ATCCCAGAAT TAATACTGGA AGACCCCAGT 
GAAGCCAAAG AGAACCTCAT TCTGCAAGAA ACATCTGTGA TAGAGTCGCT GGCTGCAGAT 
GGGAGCCCAG GGCTAAAATC AGTGCTATCT ACAAGCCGAA ATTTAAGCAA CAACTGTGAC 
ACAGGAGAGA AGCCAGTGGT TACCTTCAAA GAAAACATTA AGACACGAGA AGTGAACAGA 
GACCAAGGAA GAAGTTTTCC TCCCAAAGAG GTGAGAAGGG ACTATAGCAA AGGAATAACT 
GTAACTAAGA ATGATGGAAA GAAGGACAAC AACAAGAGGA AAACTGAAAC CAAGAAATGC 
ACCTTAGAAA AGTTACAGGA AACAGGAAAG CAGAATGTGG CAGTGCAGGT AAAATCCCAG 
ACAGAACTAA GAAAGACTCC AGTGTCTGAA GCCAGAAAAA CACCTGTAAC TCAAACCCCA 
ACTCAAGCAA GTAACTCCCA GTTCATCCCC ATTCATCACC CTGGAGCCTT CCCTCCTCTT 
CCCAGCAGGC CAGGGTTTCC GCCCCCAACA TATGTTATCC CCCCGCCTGT GGCATTTTCT 
ATGGGCTCAG GTTACACCTT CCCAGCTGGT GTTTCTGTCC CAGGAACCTT TCTTCAGCCT 
ACAGCTCACT CTCCAGCAGG AAACCAGGTG CAAGCTGGGA AACAGTCCCA CATTCCTTAC 
AGCCAGCAAC GGCCCTCTGG ACCAGGGCCA ATGAACCAGG GACCTCAACA ATCACAGCCA 
CCTTCCCAGC AACCCCTTAC ATCTTTACCA GCTCAGCCAA CAGCACAGTC TACAAGCCAG 
CTGCAGGTTC AAGCTCTAAC TCAGCAACAA CAATCCCCTA CAAAAGCTGT GCCGGCTTTG 
GGGAAAAGCC CGCCTCACCA CTCTGGATTC CAGCAGTATC AACAGGCAGA TGCCTCCAAA 
CAGCTGTGGA ATCCCCCTCA GGTTCAAGGC CCATTAGGGA AAATTATGCC TGTGAAACAG 
CCCTACTACC TTCAGACCCA AGACCCCATA AAACTGTTTG AGCCGTCATT GCAACCTCCT 
GTAATGCAGC AGCAGCCTCT AGAAAAAAAA ATGAAGCCTT TTCCCATGGA GCCATATAAC 
CATAATCCCT CAGAAGTCAA GGTCCCAGAA TTCTACTGGG ATTCTTCCTA CAGCATGGCT 
GATAACAGAT CTGTAATGGC ACAGCAAGCA AACATAGACC GCAGGGGCAA ACGGTCACCA 
GGAGTCTTCC GTCCAGAGCA GGATCCTGTA CCCAGAATGC CGTTTGAGGA CCCCAAGAGC 
TCCCCTCTGC TTCCTCCGGA CCTGTTAAAG AGTCTGGCTG CCTTGGAGGA AGAGGAAGAG 
CTGATTTTTT CTAACCCTCC TGATCTTTAC CCGGCTCTGC TGGGGCCTCT CGCCTCTCTT 
CCTGGACGAA GCCTTTTTAA ATCCTTATTG GAGAAGCCCT CAGAGCTCAT GTCACATTCA 
TCCTCTTTCC TGTCCCTCAC CGGATTCTCT CTCAATCAGG AAAGATACCC AAATAATAGT 
ATGTTCAATG AGGTATATGG GAAAAACCTG ACATCCAGCT CCAAAGCAGA ACTCAGTCCC 
TCAATGGCCC CCCAGGAAAC ATCTCTGTAT TCCCTTTTTG AAGGGACTCC GTGGTCTCCA 
TCACTTCCTG CCAGTTCAGA TCATTCAACA CCAGCCAGCC AGTCTCCTCA TTCCTCTAAC 
CCAAGCAGCC TACCCAGCTC TCCTCCAACA CACAACCATA ATTCTGTTCC ATTCTCCAAT 
TTTGGACCCA TTGGGACTCC AGATAACAGG GATAGAAGGA CTGCAGATCG GTGGAAAACT 
GATAAGCCAG CCATGGGTGG GTTTGGCATT GATTATCTCT CAGCAACGTC ATCCTCTGAG 
AGCAGTTGGC ATCAGGCCAG CACTCCGAGT GGCACCTGGA CAGGCCATGG CCCTTCCATG 
GAGGATTCCT CTGCTGTCCT CATGGAAAGC CTAAAGTCTA TCTGGTCCAG TTCCATGATG 
CATCCTGGAC CTTCTGCTCT GGAGCAGCTG TTAATGCAGC AGAAGCAGAA ACAGCAACGG 
GGACAAGGCA CCATGAACCC TCCACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005245710.1
CDS	245..3754
Translation	MTDSKLGPAEVWTSRQALQDLYQKMLVTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRANPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQTHTSAIVKPQSSSCSYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNLQKALSKALESRDEVKTKWGVSDFIKAFIKFHGHVYLSKSLEKLSPLREKLEEQFKRLLFQKAFNSQQLVHVTVINLFQLHHLRDFSNETEQHTYSQDEQLCWTQLLALFMSFLGILCKCPLQNESQEESYNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPHEEDLSSISATPLPEEFELQGFLALRPSFRNLDFSKGHQGITGDKEGQQRRIRQQRLISIGKWIADNQPRLIQCENEVGKLLFITEIPELILEDPSEAKENLILQETSVIESLAADGSPGLKSVLSTSRNLSNNCDTGEKPVVTFKENIKTREVNRDQGRSFPPKEVRRDYSKGITVTKNDGKKDNNKRKTETKKCTLEKLQETGKQNVAVQVKSQTELRKTPVSEARKTPVTQTPTQASNSQFIPIHHPGAFPPLPSRPGFPPPTYVIPPPVAFSMGSGYTFPAGVSVPGTFLQPTAHSPAGNQVQAGKQSHIPYSQQRPSGPGPMNQGPQQSQPPSQQPLTSLPAQPTAQSTSQLQVQALTQQQQSPTKAVPALGKSPPHHSGFQQYQQADASKQLWNPPQVQGPLGKIMPVKQPYYLQTQDPIKLFEPSLQPPVMQQQPLEKKMKPFPMEPYNHNPSEVKVPEFYWDSSYSMADNRSVMAQQANIDRRGKRSPGVFRPEQDPVPRMPFEDPKSSPLLPPDLLKSLAALEEEEELIFSNPPDLYPALLGPLASLPGRSLFKSLLEKPSELMSHSSSFLSLTGFSLNQERYPNNSMFNEVYGKNLTSSSKAELSPSMAPQETSLYSLFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSVPFSNFGPIGTPDNRDRRTADRWKTDKPAMGGFGIDYLSATSSSESSWHQASTPSGTWTGHGPSMEDSSAVLMESLKSIWSSSMMHPGPSALEQLLMQQKQKQQRGQGTMNPPH

Target ORF information:

RefSeq Version	XM_005245653.5
Organism	Homo sapiens(human)
Definition	Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005245653.5

ORF Insert Sequence:

ATGACAGATT CTAAGCTGGG TCCAGCTGAA GTCTGGACAT CCAGGCAGGC TCTGCAGGAC 
CTGTACCAGA AAATGCTAGT TACCGATTTG GAATACGCTT TAGACAAGAA AGTAGAACAG 
GATCTCTGGA ATCACGCCTT TAAGAATCAG ATCACAACAC TGCAAGGCCA GGCAAAGAAT 
CGAGCAAATC CGAATCGGAG TGAAGTTCAG GCAAACCTTT CTCTGTTCCT AGAGGCAGCT 
AGTGGCTTCT ATACTCAGTT ATTACAAGAA CTGTGTACAG TATTTAATGT AGATTTACCA 
TGCCGTGTGA AGTCTTCCCA ATTGGGAATT ATCAGCAATA AACAGACGCA TACCAGCGCC 
ATAGTGAAGC CACAGTCTAG CTCCTGTTCC TATATCTGCC AGCACTGCCT CGTCCACCTT 
GGAGACATTG CTCGATACAG AAACCAGACC AGCCAGGCAG AGTCCTACTA TAGGCATGCA 
GCTCAGCTTG TCCCCTCCAA TGGTCAGCCT TATAATCAGT TGGCTATCTT AGCTTCTTCC 
AAAGGAGACC ATCTGACCAC AATTTTCTAC TACTGCAGAA GCATTGCTGT GAAGTTCCCT 
TTCCCAGCTG CCTCCACTAA TCTGCAAAAA GCACTTTCTA AAGCACTGGA AAGCCGAGAT 
GAGGTGAAAA CCAAGTGGGG TGTTTCTGAC TTCATCAAGG CCTTTATTAA ATTCCACGGT 
CATGTGTACC TGAGTAAGAG CTTGGAAAAG TTGAGCCCTC TTCGAGAGAA ATTGGAAGAA 
CAGTTTAAGA GGCTGCTATT CCAAAAAGCT TTCAACTCTC AGCAGTTAGT TCATGTCACT 
GTCATTAACC TGTTTCAACT TCATCACCTT CGTGACTTTA GCAATGAAAC CGAGCAGCAC 
ACTTATAGCC AAGATGAGCA GCTATGTTGG ACACAGTTGC TGGCCCTCTT TATGTCTTTT 
CTTGGCATCC TGTGCAAGTG TCCTCTACAG AATGAGTCTC AGGAGGAGTC CTACAATGCC 
TATCCTCTTC CAGCAGTCAA GGTCTCCATG GACTGGCTAA GACTCAGACC CAGGGTCTTT 
CAGGAGGCAG TGGTGGATGA AAGACAGTAC ATTTGGCCCT GGTTGATTTC TCTTCTGAAT 
AGTTTCCATC CCCATGAAGA GGACCTCTCA AGTATTAGTG CGACACCACT TCCAGAGGAG 
TTTGAATTAC AAGGATTTTT GGCATTGAGA CCTTCTTTCA GGAACTTGGA TTTTTCCAAA 
GGTCACCAGG GTATTACAGG GGACAAAGAA GGCCAGCAAC GACGAATACG ACAGCAACGC 
TTGATCTCTA TAGGCAAATG GATTGCTGAT AATCAGCCAA GGCTGATTCA GTGTGAAAAT 
GAGGTAGGGA AATTGTTGTT TATCACAGAA ATCCCAGAAT TAATACTGGA AGACCCCAGT 
GAAGCCAAAG AGAACCTCAT TCTGCAAGAA ACATCTGTGA TAGAGTCGCT GGCTGCAGAT 
GGGAGCCCAG GGCTAAAATC AGTGCTATCT ACAAGCCGAA ATTTAAGCAA CAACTGTGAC 
ACAGGAGAGA AGCCAGTGGT TACCTTCAAA GAAAACATTA AGACACGAGA AGTGAACAGA 
GACCAAGGAA GAAGTTTTCC TCCCAAAGAG GTGAGAAGGG ACTATAGCAA AGGAATAACT 
GTAACTAAGA ATGATGGAAA GAAGGACAAC AACAAGAGGA AAACTGAAAC CAAGAAATGC 
ACCTTAGAAA AGTTACAGGA AACAGGAAAG CAGAATGTGG CAGTGCAGGT AAAATCCCAG 
ACAGAACTAA GAAAGACTCC AGTGTCTGAA GCCAGAAAAA CACCTGTAAC TCAAACCCCA 
ACTCAAGCAA GTAACTCCCA GTTCATCCCC ATTCATCACC CTGGAGCCTT CCCTCCTCTT 
CCCAGCAGGC CAGGGTTTCC GCCCCCAACA TATGTTATCC CCCCGCCTGT GGCATTTTCT 
ATGGGCTCAG GTTACACCTT CCCAGCTGGT GTTTCTGTCC CAGGAACCTT TCTTCAGCCT 
ACAGCTCACT CTCCAGCAGG AAACCAGGTG CAAGCTGGGA AACAGTCCCA CATTCCTTAC 
AGCCAGCAAC GGCCCTCTGG ACCAGGGCCA ATGAACCAGG GACCTCAACA ATCACAGCCA 
CCTTCCCAGC AACCCCTTAC ATCTTTACCA GCTCAGCCAA CAGCACAGTC TACAAGCCAG 
CTGCAGGTTC AAGCTCTAAC TCAGCAACAA CAATCCCCTA CAAAAGCTGT GCCGGCTTTG 
GGGAAAAGCC CGCCTCACCA CTCTGGATTC CAGCAGTATC AACAGGCAGA TGCCTCCAAA 
CAGCTGTGGA ATCCCCCTCA GGTTCAAGGC CCATTAGGGA AAATTATGCC TGTGAAACAG 
CCCTACTACC TTCAGACCCA AGACCCCATA AAACTGTTTG AGCCGTCATT GCAACCTCCT 
GTAATGCAGC AGCAGCCTCT AGAAAAAAAA ATGAAGCCTT TTCCCATGGA GCCATATAAC 
CATAATCCCT CAGAAGTCAA GGTCCCAGAA TTCTACTGGG ATTCTTCCTA CAGCATGGCT 
GATAACAGAT CTGTAATGGC ACAGCAAGCA AACATAGACC GCAGGGGCAA ACGGTCACCA 
GGAGTCTTCC GTCCAGAGCA GGATCCTGTA CCCAGAATGC CGTTTGAGGA CCCCAAGAGC 
TCCCCTCTGC TTCCTCCGGA CCTGTTAAAG AGTCTGGCTG CCTTGGAGGA AGAGGAAGAG 
CTGATTTTTT CTAACCCTCC TGATCTTTAC CCGGCTCTGC TGGGGCCTCT CGCCTCTCTT 
CCTGGACGAA GCCTTTTTAA ATCCTTATTG GAGAAGCCCT CAGAGCTCAT GTCACATTCA 
TCCTCTTTCC TGTCCCTCAC CGGATTCTCT CTCAATCAGG AAAGATACCC AAATAATAGT 
ATGTTCAATG AGGTATATGG GAAAAACCTG ACATCCAGCT CCAAAGCAGA ACTCAGTCCC 
TCAATGGCCC CCCAGGAAAC ATCTCTGTAT TCCCTTTTTG AAGGGACTCC GTGGTCTCCA 
TCACTTCCTG CCAGTTCAGA TCATTCAACA CCAGCCAGCC AGTCTCCTCA TTCCTCTAAC 
CCAAGCAGCC TACCCAGCTC TCCTCCAACA CACAACCATA ATTCTGTTCC ATTCTCCAAT 
TTTGGACCCA TTGGGACTCC AGATAACAGG GATAGAAGGA CTGCAGATCG GTGGAAAACT 
GATAAGCCAG CCATGGGTGG GTTTGGCATT GATTATCTCT CAGCAACGTC ATCCTCTGAG 
AGCAGTTGGC ATCAGGCCAG CACTCCGAGT GGCACCTGGA CAGGCCATGG CCCTTCCATG 
GAGGATTCCT CTGCTGTCCT CATGGAAAGC CTAAAGTCTA TCTGGTCCAG TTCCATGATG 
CATCCTGGAC CTTCTGCTCT GGAGCAGCTG TTAATGCAGC AGAAGCAGAA ACAGCAACGG 
GGACAAGGCA CCATGAACCC TCCACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG7 ( XM_006711678.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006711741.1 Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OHu34886
Clone ID Related Accession (Same CDS sequence)	NM_001375585.1 , XM_006711678.3
Accession Version	XM_006711678.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3426bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-12
Organism	Homo sapiens(human)
Product	protein SMG7 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006711678.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20190607 Annotation Version :: 109.20190607 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA Annotation Freeze :: 20190607 ##Genome-Annotation-Data-END##

ATGAGCCTGC AGAGCGCGCA GTACCTCCGG CAGGCAGAAG TCCTGAAGGC TGACATGACA 
GATTCTAAGC TGGGTCCAGC TGAAGTCTGG ACATCCAGGC AGGCTCTGCA GGACCTGTAC 
CAGAAAATGC TAGTTACCGA TTTGGAATAC GCTTTAGACA AGAAAGTAGA ACAGGATCTC 
TGGAATCACG CCTTTAAGAA TCAGATCACA ACACTGCAAG GCCAGGCAAA GAATCGAGCA 
AATCCGAATC GGAGTGAAGT TCAGGCAAAC CTTTCTCTGT TCCTAGAGGC AGCTAGTGGC 
TTCTATACTC AGTTATTACA AGAACTGTGT ACAGTATTTA ATGTAGATTT ACCATGCCGT 
GTGAAGTCTT CCCAATTGGG AATTATCAGC AATAAACAGA CGCATACCAG CGCCATAGTG 
AAGCCACAGT CTAGCTCCTG TTCCTATATC TGCCAGCACT GCCTCGTCCA CCTTGGAGAC 
ATTGCTCGAT ACAGAAACCA GACCAGCCAG GCAGAGTCCT ACTATAGGCA TGCAGCTCAG 
CTTGTCCCCT CCAATGGTCA GCCTTATAAT CAGTTGGCTA TCTTAGCTTC TTCCAAAGGA 
GACCATCTGA CCACAATTTT CTACTACTGC AGAAGCATTG CTGTGAAGTT CCCTTTCCCA 
GCTGCCTCCA CTAATCTGCA AAAAGCACTT TCTAAAGCAC TGGAAAGCCG AGATGAGGTG 
AAAACCAAGT GGGGTGTTTC TGACTTCATC AAGGCCTTTA TTAAATTCCA CGGTCATGTG 
TACCTGAGTA AGAGCTTGGA AAAGTTGAGC CCTCTTCGAG AGAAATTGGA AGAACAGTTT 
AAGAGGCTGC TATTCCAAAA AGCTTTCAAC TCTCAGCAGT TAGTTCATGT CACTGTCATT 
AACCTGTTTC AACTTCATCA CCTTCGTGAC TTTAGCAATG AAACCGAGCA GCACACTTAT 
AGCCAAGATG AGCAGCTATG TTGGACACAG TTGCTGGCCC TCTTTATGTC TTTTCTTGGC 
ATCCTGTGCA AGTGTCCTCT ACAGAATGAG TCTCAGGAGG AGTCCTACAA TGCCTATCCT 
CTTCCAGCAG TCAAGGTCTC CATGGACTGG CTAAGACTCA GACCCAGGGT CTTTCAGGAG 
GCAGTGGTGG ATGAAAGACA GTACATTTGG CCCTGGTTGA TTTCTCTTCT GAATAGTTTC 
CATCCCCATG AAGAGGACCT CTCAAGTATT AGTGCGACAC CACTTCCAGA GGAGTTTGAA 
TTACAAGGAT TTTTGGCATT GAGACCTTCT TTCAGGAACT TGGATTTTTC CAAAGGTCAC 
CAGGGTATTA CAGGGGACAA AGAAGGCCAG CAACGACGAA TACGACAGCA ACGCTTGATC 
TCTATAGGCA AATGGATTGC TGATAATCAG CCAAGGCTGA TTCAGTGTGA AAATGAGGTA 
GGGAAATTGT TGTTTATCAC AGAAATCCCA GAATTAATAC TGGAAGACCC CAGTGAAGCC 
AAAGAGAACC TCATTCTGCA AGAAACATCT GTGATAGAGT CGCTGGCTGC AGATGGGAGC 
CCAGGGCTAA AATCAGTGCT ATCTACAAGC CGAAATTTAA GCAACAACTG TGACACAGGA 
GAGAAGCCAG TGGTTACCTT CAAAGAAAAC ATTAAGACAC GAGAAGTGAA CAGAGACCAA 
GGAAGAAGTT TTCCTCCCAA AGAGGTAAAA TCCCAGACAG AACTAAGAAA GACTCCAGTG 
TCTGAAGCCA GAAAAACACC TGTAACTCAA ACCCCAACTC AAGCAAGTAA CTCCCAGTTC 
ATCCCCATTC ATCACCCTGG AGCCTTCCCT CCTCTTCCCA GCAGGCCAGG GTTTCCGCCC 
CCAACATATG TTATCCCCCC GCCTGTGGCA TTTTCTATGG GCTCAGGTTA CACCTTCCCA 
GCTGGTGTTT CTGTCCCAGG AACCTTTCTT CAGCCTACAG CTCACTCTCC AGCAGGAAAC 
CAGGTGCAAG CTGGGAAACA GTCCCACATT CCTTACAGCC AGCAACGGCC CTCTGGACCA 
GGGCCAATGA ACCAGGGACC TCAACAATCA CAGCCACCTT CCCAGCAACC CCTTACATCT 
TTACCAGCTC AGCCAACAGC ACAGTCTACA AGCCAGCTGC AGGTTCAAGC TCTAACTCAG 
CAACAACAAT CCCCTACAAA AGCTGTGCCG GCTTTGGGGA AAAGCCCGCC TCACCACTCT 
GGATTCCAGC AGTATCAACA GGCAGATGCC TCCAAACAGC TGTGGAATCC CCCTCAGGTT 
CAAGGCCCAT TAGGGAAAAT TATGCCTGTG AAACAGCCCT ACTACCTTCA GACCCAAGAC 
CCCATAAAAC TGTTTGAGCC GTCATTGCAA CCTCCTGTAA TGCAGCAGCA GCCTCTAGAA 
AAAAAAATGA AGCCTTTTCC CATGGAGCCA TATAACCATA ATCCCTCAGA AGTCAAGGTC 
CCAGAATTCT ACTGGGATTC TTCCTACAGC ATGGCTGATA ACAGATCTGT AATGGCACAG 
CAAGCAAACA TAGACCGCAG GGGCAAACGG TCACCAGGAG TCTTCCGTCC AGAGCAGGAT 
CCTGTACCCA GAATGCCGTT TGAGGACCCC AAGAGCTCCC CTCTGCTTCC TCCGGACCTG 
TTAAAGAGTC TGGCTGCCTT GGAGGAAGAG GAAGAGCTGA TTTTTTCTAA CCCTCCTGAT 
CTTTACCCGG CTCTGCTGGG GCCTCTCGCC TCTCTTCCTG GACGAAGCCT TTTTAAATCC 
TTATTGGAGA AGCCCTCAGA GCTCATGTCA CATTCATCCT CTTTCCTGTC CCTCACCGGA 
TTCTCTCTCA ATCAGGAAAG ATACCCAAAT AATAGTATGT TCAATGAGGT ATATGGGAAA 
AACCTGACAT CCAGCTCCAA AGCAGAACTC AGTCCCTCAA TGGCCCCCCA GGAAACATCT 
CTGTATTCCC TTTTTGAAGG GACTCCGTGG TCTCCATCAC TTCCTGCCAG TTCAGATCAT 
TCAACACCAG CCAGCCAGTC TCCTCATTCC TCTAACCCAA GCAGCCTACC CAGCTCTCCT 
CCAACACACA ACCATAATTC TGTTCCATTC TCCAATTTTG GACCCATTGG GACTCCAGAT 
AACAGGGATA GAAGGACTGC AGATCGGTGG AAAACTGATA AGCCAGCCAT GGGTGGGTTT 
GGCATTGATT ATCTCTCAGC AACGTCATCC TCTGAGAGCA GTTGGCATCA GGCCAGCACT 
CCGAGTGGCA CCTGGACAGG CCATGGCCCT TCCATGGAGG ATTCCTCTGC TGTCCTCATG 
GAAAGCCTAA AGTCTATCTG GTCCAGTTCC ATGATGCATC CTGGACCTTC TGCTCTGGAG 
CAGCTGTTAA TGCAGCAGAA GCAGAAACAG CAACGGGGAC AAGGCACCAT GAACCCTCCA 
CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006711741.1
CDS	120..3545
Translation	MSLQSAQYLRQAEVLKADMTDSKLGPAEVWTSRQALQDLYQKMLVTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRANPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQTHTSAIVKPQSSSCSYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNLQKALSKALESRDEVKTKWGVSDFIKAFIKFHGHVYLSKSLEKLSPLREKLEEQFKRLLFQKAFNSQQLVHVTVINLFQLHHLRDFSNETEQHTYSQDEQLCWTQLLALFMSFLGILCKCPLQNESQEESYNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPHEEDLSSISATPLPEEFELQGFLALRPSFRNLDFSKGHQGITGDKEGQQRRIRQQRLISIGKWIADNQPRLIQCENEVGKLLFITEIPELILEDPSEAKENLILQETSVIESLAADGSPGLKSVLSTSRNLSNNCDTGEKPVVTFKENIKTREVNRDQGRSFPPKEVKSQTELRKTPVSEARKTPVTQTPTQASNSQFIPIHHPGAFPPLPSRPGFPPPTYVIPPPVAFSMGSGYTFPAGVSVPGTFLQPTAHSPAGNQVQAGKQSHIPYSQQRPSGPGPMNQGPQQSQPPSQQPLTSLPAQPTAQSTSQLQVQALTQQQQSPTKAVPALGKSPPHHSGFQQYQQADASKQLWNPPQVQGPLGKIMPVKQPYYLQTQDPIKLFEPSLQPPVMQQQPLEKKMKPFPMEPYNHNPSEVKVPEFYWDSSYSMADNRSVMAQQANIDRRGKRSPGVFRPEQDPVPRMPFEDPKSSPLLPPDLLKSLAALEEEEELIFSNPPDLYPALLGPLASLPGRSLFKSLLEKPSELMSHSSSFLSLTGFSLNQERYPNNSMFNEVYGKNLTSSSKAELSPSMAPQETSLYSLFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSVPFSNFGPIGTPDNRDRRTADRWKTDKPAMGGFGIDYLSATSSSESSWHQASTPSGTWTGHGPSMEDSSAVLMESLKSIWSSSMMHPGPSALEQLLMQQKQKQQRGQGTMNPPH

Target ORF information:

RefSeq Version	XM_006711678.3
Organism	Homo sapiens(human)
Definition	Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006711678.3

ORF Insert Sequence:

ATGAGCCTGC AGAGCGCGCA GTACCTCCGG CAGGCAGAAG TCCTGAAGGC TGACATGACA 
GATTCTAAGC TGGGTCCAGC TGAAGTCTGG ACATCCAGGC AGGCTCTGCA GGACCTGTAC 
CAGAAAATGC TAGTTACCGA TTTGGAATAC GCTTTAGACA AGAAAGTAGA ACAGGATCTC 
TGGAATCACG CCTTTAAGAA TCAGATCACA ACACTGCAAG GCCAGGCAAA GAATCGAGCA 
AATCCGAATC GGAGTGAAGT TCAGGCAAAC CTTTCTCTGT TCCTAGAGGC AGCTAGTGGC 
TTCTATACTC AGTTATTACA AGAACTGTGT ACAGTATTTA ATGTAGATTT ACCATGCCGT 
GTGAAGTCTT CCCAATTGGG AATTATCAGC AATAAACAGA CGCATACCAG CGCCATAGTG 
AAGCCACAGT CTAGCTCCTG TTCCTATATC TGCCAGCACT GCCTCGTCCA CCTTGGAGAC 
ATTGCTCGAT ACAGAAACCA GACCAGCCAG GCAGAGTCCT ACTATAGGCA TGCAGCTCAG 
CTTGTCCCCT CCAATGGTCA GCCTTATAAT CAGTTGGCTA TCTTAGCTTC TTCCAAAGGA 
GACCATCTGA CCACAATTTT CTACTACTGC AGAAGCATTG CTGTGAAGTT CCCTTTCCCA 
GCTGCCTCCA CTAATCTGCA AAAAGCACTT TCTAAAGCAC TGGAAAGCCG AGATGAGGTG 
AAAACCAAGT GGGGTGTTTC TGACTTCATC AAGGCCTTTA TTAAATTCCA CGGTCATGTG 
TACCTGAGTA AGAGCTTGGA AAAGTTGAGC CCTCTTCGAG AGAAATTGGA AGAACAGTTT 
AAGAGGCTGC TATTCCAAAA AGCTTTCAAC TCTCAGCAGT TAGTTCATGT CACTGTCATT 
AACCTGTTTC AACTTCATCA CCTTCGTGAC TTTAGCAATG AAACCGAGCA GCACACTTAT 
AGCCAAGATG AGCAGCTATG TTGGACACAG TTGCTGGCCC TCTTTATGTC TTTTCTTGGC 
ATCCTGTGCA AGTGTCCTCT ACAGAATGAG TCTCAGGAGG AGTCCTACAA TGCCTATCCT 
CTTCCAGCAG TCAAGGTCTC CATGGACTGG CTAAGACTCA GACCCAGGGT CTTTCAGGAG 
GCAGTGGTGG ATGAAAGACA GTACATTTGG CCCTGGTTGA TTTCTCTTCT GAATAGTTTC 
CATCCCCATG AAGAGGACCT CTCAAGTATT AGTGCGACAC CACTTCCAGA GGAGTTTGAA 
TTACAAGGAT TTTTGGCATT GAGACCTTCT TTCAGGAACT TGGATTTTTC CAAAGGTCAC 
CAGGGTATTA CAGGGGACAA AGAAGGCCAG CAACGACGAA TACGACAGCA ACGCTTGATC 
TCTATAGGCA AATGGATTGC TGATAATCAG CCAAGGCTGA TTCAGTGTGA AAATGAGGTA 
GGGAAATTGT TGTTTATCAC AGAAATCCCA GAATTAATAC TGGAAGACCC CAGTGAAGCC 
AAAGAGAACC TCATTCTGCA AGAAACATCT GTGATAGAGT CGCTGGCTGC AGATGGGAGC 
CCAGGGCTAA AATCAGTGCT ATCTACAAGC CGAAATTTAA GCAACAACTG TGACACAGGA 
GAGAAGCCAG TGGTTACCTT CAAAGAAAAC ATTAAGACAC GAGAAGTGAA CAGAGACCAA 
GGAAGAAGTT TTCCTCCCAA AGAGGTAAAA TCCCAGACAG AACTAAGAAA GACTCCAGTG 
TCTGAAGCCA GAAAAACACC TGTAACTCAA ACCCCAACTC AAGCAAGTAA CTCCCAGTTC 
ATCCCCATTC ATCACCCTGG AGCCTTCCCT CCTCTTCCCA GCAGGCCAGG GTTTCCGCCC 
CCAACATATG TTATCCCCCC GCCTGTGGCA TTTTCTATGG GCTCAGGTTA CACCTTCCCA 
GCTGGTGTTT CTGTCCCAGG AACCTTTCTT CAGCCTACAG CTCACTCTCC AGCAGGAAAC 
CAGGTGCAAG CTGGGAAACA GTCCCACATT CCTTACAGCC AGCAACGGCC CTCTGGACCA 
GGGCCAATGA ACCAGGGACC TCAACAATCA CAGCCACCTT CCCAGCAACC CCTTACATCT 
TTACCAGCTC AGCCAACAGC ACAGTCTACA AGCCAGCTGC AGGTTCAAGC TCTAACTCAG 
CAACAACAAT CCCCTACAAA AGCTGTGCCG GCTTTGGGGA AAAGCCCGCC TCACCACTCT 
GGATTCCAGC AGTATCAACA GGCAGATGCC TCCAAACAGC TGTGGAATCC CCCTCAGGTT 
CAAGGCCCAT TAGGGAAAAT TATGCCTGTG AAACAGCCCT ACTACCTTCA GACCCAAGAC 
CCCATAAAAC TGTTTGAGCC GTCATTGCAA CCTCCTGTAA TGCAGCAGCA GCCTCTAGAA 
AAAAAAATGA AGCCTTTTCC CATGGAGCCA TATAACCATA ATCCCTCAGA AGTCAAGGTC 
CCAGAATTCT ACTGGGATTC TTCCTACAGC ATGGCTGATA ACAGATCTGT AATGGCACAG 
CAAGCAAACA TAGACCGCAG GGGCAAACGG TCACCAGGAG TCTTCCGTCC AGAGCAGGAT 
CCTGTACCCA GAATGCCGTT TGAGGACCCC AAGAGCTCCC CTCTGCTTCC TCCGGACCTG 
TTAAAGAGTC TGGCTGCCTT GGAGGAAGAG GAAGAGCTGA TTTTTTCTAA CCCTCCTGAT 
CTTTACCCGG CTCTGCTGGG GCCTCTCGCC TCTCTTCCTG GACGAAGCCT TTTTAAATCC 
TTATTGGAGA AGCCCTCAGA GCTCATGTCA CATTCATCCT CTTTCCTGTC CCTCACCGGA 
TTCTCTCTCA ATCAGGAAAG ATACCCAAAT AATAGTATGT TCAATGAGGT ATATGGGAAA 
AACCTGACAT CCAGCTCCAA AGCAGAACTC AGTCCCTCAA TGGCCCCCCA GGAAACATCT 
CTGTATTCCC TTTTTGAAGG GACTCCGTGG TCTCCATCAC TTCCTGCCAG TTCAGATCAT 
TCAACACCAG CCAGCCAGTC TCCTCATTCC TCTAACCCAA GCAGCCTACC CAGCTCTCCT 
CCAACACACA ACCATAATTC TGTTCCATTC TCCAATTTTG GACCCATTGG GACTCCAGAT 
AACAGGGATA GAAGGACTGC AGATCGGTGG AAAACTGATA AGCCAGCCAT GGGTGGGTTT 
GGCATTGATT ATCTCTCAGC AACGTCATCC TCTGAGAGCA GTTGGCATCA GGCCAGCACT 
CCGAGTGGCA CCTGGACAGG CCATGGCCCT TCCATGGAGG ATTCCTCTGC TGTCCTCATG 
GAAAGCCTAA AGTCTATCTG GTCCAGTTCC ATGATGCATC CTGGACCTTC TGCTCTGGAG 
CAGCTGTTAA TGCAGCAGAA GCAGAAACAG CAACGGGGAC AAGGCACCAT GAACCCTCCA 
CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG7 ( XM_011510208.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011508510.1 Homo sapiens SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X8, mRNA.

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CloneID	OHu63454
Clone ID Related Accession (Same CDS sequence)	XM_011510208.2 , XM_011510208.3
Accession Version	XM_011510208.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3501bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	protein SMG7 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011510208.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
CAGATGAGGA CCGAAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG 
GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCTGA AGTCTGGACA 
TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTAG TTACCGATTT GGAATACGCT 
TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCACGCCT TTAAGAATCA GATCACAACA 
CTGCAAGGCC AGGCAAAGAA TCGAGCAAAT CCGAATCGGA GTGAAGTTCA GGCAAACCTT 
TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA 
GTATTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AATTGGGAAT TATCAGCAAT 
AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC 
CAGCACTGCC TCGTCCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA 
GAGTCCTACT ATAGGCATGC AGCTCAGCTT GTCCCCTCCA ATGGTCAGCC TTATAATCAG 
TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACCA CAATTTTCTA CTACTGCAGA 
AGCATTGCTG TGAAGTTCCC TTTCCCAGCT GCCTCCACTA ATCTGCAAAA AGCACTTTCT 
AAAGCACTGG AAAGCCGAGA TGAGGTGAAA ACCAAGTGGG GTGTTTCTGA CTTCATCAAG 
GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT 
CTTCGAGAGA AATTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAAAAAGC TTTCAACTCT 
CAGCAGTTAG TTCATGTCAC TGTCATTAAC CTGTTTCAAC TTCATCACCT TCGTGACTTT 
AGCAATGAAA CCGAGCAGCA CACTTATAGC CAAGATGAGC AGCTATGTTG GACACAGTTG 
CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTACA GAATGAGTCT 
CAGGAGGAGT CCTACAATGC CTATCCTCTT CCAGCAGTCA AGGTCTCCAT GGACTGGCTA 
AGACTCAGAC CCAGGGTCTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCC 
TGGTTGATTT CTCTTCTGAA TAGTTTCCAT CCCCATGAAG AGGACCTCTC AAGTATTAGT 
GCGACACCAC TTCCAGAGGA GTTTGAATTA CAAGGATTTT TGGCATTGAG ACCTTCTTTC 
AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GGGACAAAGA AGGCCAGCAA 
CGACGAATAC GACAGCAACG CTTGATCTCT ATAGGCAAAT GGATTGCTGA TAATCAGCCA 
AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAA 
TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA AACATCTGTG 
ATAGAGTCGC TGGCTGCAGA TGGGAGCCCA GGGCTAAAAT CAGTGCTATC TACAAGCCGA 
AATTTAAGCA ACAACTGTGA CACAGGAGAG AAGCCAGTGG TTACCTTCAA AGAAAACATT 
AAGACACGAG AAGTGAACAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTGAGAAGG 
GACTATAGCA AAGGAATAAC TGTAACTAAG AATGATGGAA AGAAGGACAA CAACAAGAGG 
AAAACTGAAA CCAAGAAATG CACCTTAGAA AAGTTACAGG AAACAGGAAA GCAGAATGTG 
GCAGTGCAGG TAAAATCCCA GACAGAACTA AGAAAGACTC CAGTGTCTGA AGCCAGAAAA 
ACACCTGTAA CTCAAACCCC AACTCAAGCA AGTAACTCCC AGTTCATCCC CATTCATCAC 
CCTGGAGCCT TCCCTCCTCT TCCCAGCAGG CCAGGGTTTC CGCCCCCAAC ATATGTTATC 
CCCCCGCCTG TGGCATTTTC TATGGGCTCA GGTTACACCT TCCCAGCTGG TGTTTCTGTC 
CCAGGAACCT TTCTTCAGCC TACAGCTCAC TCTCCAGCAG GAAACCAGGT GCAAGCTGGG 
AAACAGTCCC ACATTCCTTA CAGCCAGCAA CGGCCCTCTG GACCAGGGCC AATGAACCAG 
GGACCTCAAC AATCACAGCC ACCTTCCCAG CAACCCCTTA CATCTTTACC AGCTCAGCCA 
ACAGCACAGT CTACAAGCCA GCTGCAGGTT CAAGCTCTAA CTCAGCAACA ACAATCCCCT 
ACAAAAGCTG TGCCGGCTTT GGGGAAAAGC CCGCCTCACC ACTCTGGATT CCAGCAGTAT 
CAACAGGCAG ATGCCTCCAA ACAGCTGTGG AATCCCCCTC AGGTTCAAGG CCCATTAGGG 
AAAATTATGC CTGTGAAACA GCCCTACTAC CTTCAGACCC AAGACCCCAT AAAACTGTTT 
GAGCCGTCAT TGCAACCTCC TGTAATGCAG CAGCAGCCTC TAGAAAAAAA AATGAAGCCT 
TTTCCCATGG AGCCATATAA CCATAATCCC TCAGAAGTCA AGGTCCCAGA ATTCTACTGG 
GATTCTTCCT ACAGCATGGC TGATAACAGA TCTGTAATGG CACAGCAAGC AAACATAGAC 
CGCAGGGGCA AACGGTCACC AGGAGTCTTC CGTCCAGAGC AGGATCCTGT ACCCAGAATG 
CCGTTTGAGA AATCCTTATT GGAGAAGCCC TCAGAGCTCA TGTCACATTC ATCCTCTTTC 
CTGTCCCTCA CCGGATTCTC TCTCAATCAG GAAAGATACC CAAATAATAG TATGTTCAAT 
GAGGTATATG GGAAAAACCT GACATCCAGC TCCAAAGCAG AACTCAGTCC CTCAATGGCC 
CCCCAGGAAA CATCTCTGTA TTCCCTTTTT GAAGGGACTC CGTGGTCTCC ATCACTTCCT 
GCCAGTTCAG ATCATTCAAC ACCAGCCAGC CAGTCTCCTC ATTCCTCTAA CCCAAGCAGC 
CTACCCAGCT CTCCTCCAAC ACACAACCAT AATTCTGTTC CATTCTCCAA TTTTGGACCC 
ATTGGGACTC CAGATAACAG GGATAGAAGG ACTGCAGATC GGTGGAAAAC TGATAAGCCA 
GCCATGGGTG GGTTTGGCAT TGATTATCTC TCAGCAACGT CATCCTCTGA GAGCAGTTGG 
CATCAGGCCA GCACTCCGAG TGGCACCTGG ACAGGCCATG GCCCTTCCAT GGAGGATTCC 
TCTGCTGTCC TCATGGAAAG CCTAAAGTCT ATCTGGTCCA GTTCCATGAT GCATCCTGGA 
CCTTCTGCTC TGGAGCAGCT GTTAATGCAG CAGAAGCAGA AACAGCAACG GGGACAAGGC 
ACCATGAACC CTCCACACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011508510.1
CDS	1027..4527
Translation	MRNWMFLQIVIVGENVSFKSQMRTENLKSEEHLKSSNIRQAEVLKADMTDSKLGPAEVWTSRQALQDLYQKMLVTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRANPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQTHTSAIVKPQSSSCSYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNLQKALSKALESRDEVKTKWGVSDFIKAFIKFHGHVYLSKSLEKLSPLREKLEEQFKRLLFQKAFNSQQLVHVTVINLFQLHHLRDFSNETEQHTYSQDEQLCWTQLLALFMSFLGILCKCPLQNESQEESYNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPHEEDLSSISATPLPEEFELQGFLALRPSFRNLDFSKGHQGITGDKEGQQRRIRQQRLISIGKWIADNQPRLIQCENEVGKLLFITEIPELILEDPSEAKENLILQETSVIESLAADGSPGLKSVLSTSRNLSNNCDTGEKPVVTFKENIKTREVNRDQGRSFPPKEVRRDYSKGITVTKNDGKKDNNKRKTETKKCTLEKLQETGKQNVAVQVKSQTELRKTPVSEARKTPVTQTPTQASNSQFIPIHHPGAFPPLPSRPGFPPPTYVIPPPVAFSMGSGYTFPAGVSVPGTFLQPTAHSPAGNQVQAGKQSHIPYSQQRPSGPGPMNQGPQQSQPPSQQPLTSLPAQPTAQSTSQLQVQALTQQQQSPTKAVPALGKSPPHHSGFQQYQQADASKQLWNPPQVQGPLGKIMPVKQPYYLQTQDPIKLFEPSLQPPVMQQQPLEKKMKPFPMEPYNHNPSEVKVPEFYWDSSYSMADNRSVMAQQANIDRRGKRSPGVFRPEQDPVPRMPFEKSLLEKPSELMSHSSSFLSLTGFSLNQERYPNNSMFNEVYGKNLTSSSKAELSPSMAPQETSLYSLFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSVPFSNFGPIGTPDNRDRRTADRWKTDKPAMGGFGIDYLSATSSSESSWHQASTPSGTWTGHGPSMEDSSAVLMESLKSIWSSSMMHPGPSALEQLLMQQKQKQQRGQGTMNPPH

Target ORF information:

RefSeq Version	XM_011510208.2
Organism	Homo sapiens(human)
Definition	Homo sapiens SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011510208.2

ORF Insert Sequence:

ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
CAGATGAGGA CCGAAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG 
GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCTGA AGTCTGGACA 
TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTAG TTACCGATTT GGAATACGCT 
TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCACGCCT TTAAGAATCA GATCACAACA 
CTGCAAGGCC AGGCAAAGAA TCGAGCAAAT CCGAATCGGA GTGAAGTTCA GGCAAACCTT 
TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA 
GTATTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AATTGGGAAT TATCAGCAAT 
AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC 
CAGCACTGCC TCGTCCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA 
GAGTCCTACT ATAGGCATGC AGCTCAGCTT GTCCCCTCCA ATGGTCAGCC TTATAATCAG 
TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACCA CAATTTTCTA CTACTGCAGA 
AGCATTGCTG TGAAGTTCCC TTTCCCAGCT GCCTCCACTA ATCTGCAAAA AGCACTTTCT 
AAAGCACTGG AAAGCCGAGA TGAGGTGAAA ACCAAGTGGG GTGTTTCTGA CTTCATCAAG 
GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT 
CTTCGAGAGA AATTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAAAAAGC TTTCAACTCT 
CAGCAGTTAG TTCATGTCAC TGTCATTAAC CTGTTTCAAC TTCATCACCT TCGTGACTTT 
AGCAATGAAA CCGAGCAGCA CACTTATAGC CAAGATGAGC AGCTATGTTG GACACAGTTG 
CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTACA GAATGAGTCT 
CAGGAGGAGT CCTACAATGC CTATCCTCTT CCAGCAGTCA AGGTCTCCAT GGACTGGCTA 
AGACTCAGAC CCAGGGTCTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCC 
TGGTTGATTT CTCTTCTGAA TAGTTTCCAT CCCCATGAAG AGGACCTCTC AAGTATTAGT 
GCGACACCAC TTCCAGAGGA GTTTGAATTA CAAGGATTTT TGGCATTGAG ACCTTCTTTC 
AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GGGACAAAGA AGGCCAGCAA 
CGACGAATAC GACAGCAACG CTTGATCTCT ATAGGCAAAT GGATTGCTGA TAATCAGCCA 
AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAA 
TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA AACATCTGTG 
ATAGAGTCGC TGGCTGCAGA TGGGAGCCCA GGGCTAAAAT CAGTGCTATC TACAAGCCGA 
AATTTAAGCA ACAACTGTGA CACAGGAGAG AAGCCAGTGG TTACCTTCAA AGAAAACATT 
AAGACACGAG AAGTGAACAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTGAGAAGG 
GACTATAGCA AAGGAATAAC TGTAACTAAG AATGATGGAA AGAAGGACAA CAACAAGAGG 
AAAACTGAAA CCAAGAAATG CACCTTAGAA AAGTTACAGG AAACAGGAAA GCAGAATGTG 
GCAGTGCAGG TAAAATCCCA GACAGAACTA AGAAAGACTC CAGTGTCTGA AGCCAGAAAA 
ACACCTGTAA CTCAAACCCC AACTCAAGCA AGTAACTCCC AGTTCATCCC CATTCATCAC 
CCTGGAGCCT TCCCTCCTCT TCCCAGCAGG CCAGGGTTTC CGCCCCCAAC ATATGTTATC 
CCCCCGCCTG TGGCATTTTC TATGGGCTCA GGTTACACCT TCCCAGCTGG TGTTTCTGTC 
CCAGGAACCT TTCTTCAGCC TACAGCTCAC TCTCCAGCAG GAAACCAGGT GCAAGCTGGG 
AAACAGTCCC ACATTCCTTA CAGCCAGCAA CGGCCCTCTG GACCAGGGCC AATGAACCAG 
GGACCTCAAC AATCACAGCC ACCTTCCCAG CAACCCCTTA CATCTTTACC AGCTCAGCCA 
ACAGCACAGT CTACAAGCCA GCTGCAGGTT CAAGCTCTAA CTCAGCAACA ACAATCCCCT 
ACAAAAGCTG TGCCGGCTTT GGGGAAAAGC CCGCCTCACC ACTCTGGATT CCAGCAGTAT 
CAACAGGCAG ATGCCTCCAA ACAGCTGTGG AATCCCCCTC AGGTTCAAGG CCCATTAGGG 
AAAATTATGC CTGTGAAACA GCCCTACTAC CTTCAGACCC AAGACCCCAT AAAACTGTTT 
GAGCCGTCAT TGCAACCTCC TGTAATGCAG CAGCAGCCTC TAGAAAAAAA AATGAAGCCT 
TTTCCCATGG AGCCATATAA CCATAATCCC TCAGAAGTCA AGGTCCCAGA ATTCTACTGG 
GATTCTTCCT ACAGCATGGC TGATAACAGA TCTGTAATGG CACAGCAAGC AAACATAGAC 
CGCAGGGGCA AACGGTCACC AGGAGTCTTC CGTCCAGAGC AGGATCCTGT ACCCAGAATG 
CCGTTTGAGA AATCCTTATT GGAGAAGCCC TCAGAGCTCA TGTCACATTC ATCCTCTTTC 
CTGTCCCTCA CCGGATTCTC TCTCAATCAG GAAAGATACC CAAATAATAG TATGTTCAAT 
GAGGTATATG GGAAAAACCT GACATCCAGC TCCAAAGCAG AACTCAGTCC CTCAATGGCC 
CCCCAGGAAA CATCTCTGTA TTCCCTTTTT GAAGGGACTC CGTGGTCTCC ATCACTTCCT 
GCCAGTTCAG ATCATTCAAC ACCAGCCAGC CAGTCTCCTC ATTCCTCTAA CCCAAGCAGC 
CTACCCAGCT CTCCTCCAAC ACACAACCAT AATTCTGTTC CATTCTCCAA TTTTGGACCC 
ATTGGGACTC CAGATAACAG GGATAGAAGG ACTGCAGATC GGTGGAAAAC TGATAAGCCA 
GCCATGGGTG GGTTTGGCAT TGATTATCTC TCAGCAACGT CATCCTCTGA GAGCAGTTGG 
CATCAGGCCA GCACTCCGAG TGGCACCTGG ACAGGCCATG GCCCTTCCAT GGAGGATTCC 
TCTGCTGTCC TCATGGAAAG CCTAAAGTCT ATCTGGTCCA GTTCCATGAT GCATCCTGGA 
CCTTCTGCTC TGGAGCAGCT GTTAATGCAG CAGAAGCAGA AACAGCAACG GGGACAAGGC 
ACCATGAACC CTCCACACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG7 ( XM_011510208.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011508510.1 Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OHu63454
Clone ID Related Accession (Same CDS sequence)	XM_011510208.2 , XM_011510208.3
Accession Version	XM_011510208.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3501bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein SMG7 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011510208.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
CAGATGAGGA CCGAAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG 
GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCTGA AGTCTGGACA 
TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTAG TTACCGATTT GGAATACGCT 
TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCACGCCT TTAAGAATCA GATCACAACA 
CTGCAAGGCC AGGCAAAGAA TCGAGCAAAT CCGAATCGGA GTGAAGTTCA GGCAAACCTT 
TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA 
GTATTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AATTGGGAAT TATCAGCAAT 
AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC 
CAGCACTGCC TCGTCCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA 
GAGTCCTACT ATAGGCATGC AGCTCAGCTT GTCCCCTCCA ATGGTCAGCC TTATAATCAG 
TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACCA CAATTTTCTA CTACTGCAGA 
AGCATTGCTG TGAAGTTCCC TTTCCCAGCT GCCTCCACTA ATCTGCAAAA AGCACTTTCT 
AAAGCACTGG AAAGCCGAGA TGAGGTGAAA ACCAAGTGGG GTGTTTCTGA CTTCATCAAG 
GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT 
CTTCGAGAGA AATTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAAAAAGC TTTCAACTCT 
CAGCAGTTAG TTCATGTCAC TGTCATTAAC CTGTTTCAAC TTCATCACCT TCGTGACTTT 
AGCAATGAAA CCGAGCAGCA CACTTATAGC CAAGATGAGC AGCTATGTTG GACACAGTTG 
CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTACA GAATGAGTCT 
CAGGAGGAGT CCTACAATGC CTATCCTCTT CCAGCAGTCA AGGTCTCCAT GGACTGGCTA 
AGACTCAGAC CCAGGGTCTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCC 
TGGTTGATTT CTCTTCTGAA TAGTTTCCAT CCCCATGAAG AGGACCTCTC AAGTATTAGT 
GCGACACCAC TTCCAGAGGA GTTTGAATTA CAAGGATTTT TGGCATTGAG ACCTTCTTTC 
AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GGGACAAAGA AGGCCAGCAA 
CGACGAATAC GACAGCAACG CTTGATCTCT ATAGGCAAAT GGATTGCTGA TAATCAGCCA 
AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAA 
TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA AACATCTGTG 
ATAGAGTCGC TGGCTGCAGA TGGGAGCCCA GGGCTAAAAT CAGTGCTATC TACAAGCCGA 
AATTTAAGCA ACAACTGTGA CACAGGAGAG AAGCCAGTGG TTACCTTCAA AGAAAACATT 
AAGACACGAG AAGTGAACAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTGAGAAGG 
GACTATAGCA AAGGAATAAC TGTAACTAAG AATGATGGAA AGAAGGACAA CAACAAGAGG 
AAAACTGAAA CCAAGAAATG CACCTTAGAA AAGTTACAGG AAACAGGAAA GCAGAATGTG 
GCAGTGCAGG TAAAATCCCA GACAGAACTA AGAAAGACTC CAGTGTCTGA AGCCAGAAAA 
ACACCTGTAA CTCAAACCCC AACTCAAGCA AGTAACTCCC AGTTCATCCC CATTCATCAC 
CCTGGAGCCT TCCCTCCTCT TCCCAGCAGG CCAGGGTTTC CGCCCCCAAC ATATGTTATC 
CCCCCGCCTG TGGCATTTTC TATGGGCTCA GGTTACACCT TCCCAGCTGG TGTTTCTGTC 
CCAGGAACCT TTCTTCAGCC TACAGCTCAC TCTCCAGCAG GAAACCAGGT GCAAGCTGGG 
AAACAGTCCC ACATTCCTTA CAGCCAGCAA CGGCCCTCTG GACCAGGGCC AATGAACCAG 
GGACCTCAAC AATCACAGCC ACCTTCCCAG CAACCCCTTA CATCTTTACC AGCTCAGCCA 
ACAGCACAGT CTACAAGCCA GCTGCAGGTT CAAGCTCTAA CTCAGCAACA ACAATCCCCT 
ACAAAAGCTG TGCCGGCTTT GGGGAAAAGC CCGCCTCACC ACTCTGGATT CCAGCAGTAT 
CAACAGGCAG ATGCCTCCAA ACAGCTGTGG AATCCCCCTC AGGTTCAAGG CCCATTAGGG 
AAAATTATGC CTGTGAAACA GCCCTACTAC CTTCAGACCC AAGACCCCAT AAAACTGTTT 
GAGCCGTCAT TGCAACCTCC TGTAATGCAG CAGCAGCCTC TAGAAAAAAA AATGAAGCCT 
TTTCCCATGG AGCCATATAA CCATAATCCC TCAGAAGTCA AGGTCCCAGA ATTCTACTGG 
GATTCTTCCT ACAGCATGGC TGATAACAGA TCTGTAATGG CACAGCAAGC AAACATAGAC 
CGCAGGGGCA AACGGTCACC AGGAGTCTTC CGTCCAGAGC AGGATCCTGT ACCCAGAATG 
CCGTTTGAGA AATCCTTATT GGAGAAGCCC TCAGAGCTCA TGTCACATTC ATCCTCTTTC 
CTGTCCCTCA CCGGATTCTC TCTCAATCAG GAAAGATACC CAAATAATAG TATGTTCAAT 
GAGGTATATG GGAAAAACCT GACATCCAGC TCCAAAGCAG AACTCAGTCC CTCAATGGCC 
CCCCAGGAAA CATCTCTGTA TTCCCTTTTT GAAGGGACTC CGTGGTCTCC ATCACTTCCT 
GCCAGTTCAG ATCATTCAAC ACCAGCCAGC CAGTCTCCTC ATTCCTCTAA CCCAAGCAGC 
CTACCCAGCT CTCCTCCAAC ACACAACCAT AATTCTGTTC CATTCTCCAA TTTTGGACCC 
ATTGGGACTC CAGATAACAG GGATAGAAGG ACTGCAGATC GGTGGAAAAC TGATAAGCCA 
GCCATGGGTG GGTTTGGCAT TGATTATCTC TCAGCAACGT CATCCTCTGA GAGCAGTTGG 
CATCAGGCCA GCACTCCGAG TGGCACCTGG ACAGGCCATG GCCCTTCCAT GGAGGATTCC 
TCTGCTGTCC TCATGGAAAG CCTAAAGTCT ATCTGGTCCA GTTCCATGAT GCATCCTGGA 
CCTTCTGCTC TGGAGCAGCT GTTAATGCAG CAGAAGCAGA AACAGCAACG GGGACAAGGC 
ACCATGAACC CTCCACACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011508510.1
CDS	996..4496
Translation	MRNWMFLQIVIVGENVSFKSQMRTENLKSEEHLKSSNIRQAEVLKADMTDSKLGPAEVWTSRQALQDLYQKMLVTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRANPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQTHTSAIVKPQSSSCSYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNLQKALSKALESRDEVKTKWGVSDFIKAFIKFHGHVYLSKSLEKLSPLREKLEEQFKRLLFQKAFNSQQLVHVTVINLFQLHHLRDFSNETEQHTYSQDEQLCWTQLLALFMSFLGILCKCPLQNESQEESYNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPHEEDLSSISATPLPEEFELQGFLALRPSFRNLDFSKGHQGITGDKEGQQRRIRQQRLISIGKWIADNQPRLIQCENEVGKLLFITEIPELILEDPSEAKENLILQETSVIESLAADGSPGLKSVLSTSRNLSNNCDTGEKPVVTFKENIKTREVNRDQGRSFPPKEVRRDYSKGITVTKNDGKKDNNKRKTETKKCTLEKLQETGKQNVAVQVKSQTELRKTPVSEARKTPVTQTPTQASNSQFIPIHHPGAFPPLPSRPGFPPPTYVIPPPVAFSMGSGYTFPAGVSVPGTFLQPTAHSPAGNQVQAGKQSHIPYSQQRPSGPGPMNQGPQQSQPPSQQPLTSLPAQPTAQSTSQLQVQALTQQQQSPTKAVPALGKSPPHHSGFQQYQQADASKQLWNPPQVQGPLGKIMPVKQPYYLQTQDPIKLFEPSLQPPVMQQQPLEKKMKPFPMEPYNHNPSEVKVPEFYWDSSYSMADNRSVMAQQANIDRRGKRSPGVFRPEQDPVPRMPFEKSLLEKPSELMSHSSSFLSLTGFSLNQERYPNNSMFNEVYGKNLTSSSKAELSPSMAPQETSLYSLFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSVPFSNFGPIGTPDNRDRRTADRWKTDKPAMGGFGIDYLSATSSSESSWHQASTPSGTWTGHGPSMEDSSAVLMESLKSIWSSSMMHPGPSALEQLLMQQKQKQQRGQGTMNPPH

Target ORF information:

RefSeq Version	XM_011510208.3
Organism	Homo sapiens(human)
Definition	Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011510208.3

ORF Insert Sequence:

ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
CAGATGAGGA CCGAAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG 
GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCTGA AGTCTGGACA 
TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTAG TTACCGATTT GGAATACGCT 
TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCACGCCT TTAAGAATCA GATCACAACA 
CTGCAAGGCC AGGCAAAGAA TCGAGCAAAT CCGAATCGGA GTGAAGTTCA GGCAAACCTT 
TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA 
GTATTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AATTGGGAAT TATCAGCAAT 
AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC 
CAGCACTGCC TCGTCCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA 
GAGTCCTACT ATAGGCATGC AGCTCAGCTT GTCCCCTCCA ATGGTCAGCC TTATAATCAG 
TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACCA CAATTTTCTA CTACTGCAGA 
AGCATTGCTG TGAAGTTCCC TTTCCCAGCT GCCTCCACTA ATCTGCAAAA AGCACTTTCT 
AAAGCACTGG AAAGCCGAGA TGAGGTGAAA ACCAAGTGGG GTGTTTCTGA CTTCATCAAG 
GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT 
CTTCGAGAGA AATTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAAAAAGC TTTCAACTCT 
CAGCAGTTAG TTCATGTCAC TGTCATTAAC CTGTTTCAAC TTCATCACCT TCGTGACTTT 
AGCAATGAAA CCGAGCAGCA CACTTATAGC CAAGATGAGC AGCTATGTTG GACACAGTTG 
CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTACA GAATGAGTCT 
CAGGAGGAGT CCTACAATGC CTATCCTCTT CCAGCAGTCA AGGTCTCCAT GGACTGGCTA 
AGACTCAGAC CCAGGGTCTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCC 
TGGTTGATTT CTCTTCTGAA TAGTTTCCAT CCCCATGAAG AGGACCTCTC AAGTATTAGT 
GCGACACCAC TTCCAGAGGA GTTTGAATTA CAAGGATTTT TGGCATTGAG ACCTTCTTTC 
AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GGGACAAAGA AGGCCAGCAA 
CGACGAATAC GACAGCAACG CTTGATCTCT ATAGGCAAAT GGATTGCTGA TAATCAGCCA 
AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAA 
TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA AACATCTGTG 
ATAGAGTCGC TGGCTGCAGA TGGGAGCCCA GGGCTAAAAT CAGTGCTATC TACAAGCCGA 
AATTTAAGCA ACAACTGTGA CACAGGAGAG AAGCCAGTGG TTACCTTCAA AGAAAACATT 
AAGACACGAG AAGTGAACAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTGAGAAGG 
GACTATAGCA AAGGAATAAC TGTAACTAAG AATGATGGAA AGAAGGACAA CAACAAGAGG 
AAAACTGAAA CCAAGAAATG CACCTTAGAA AAGTTACAGG AAACAGGAAA GCAGAATGTG 
GCAGTGCAGG TAAAATCCCA GACAGAACTA AGAAAGACTC CAGTGTCTGA AGCCAGAAAA 
ACACCTGTAA CTCAAACCCC AACTCAAGCA AGTAACTCCC AGTTCATCCC CATTCATCAC 
CCTGGAGCCT TCCCTCCTCT TCCCAGCAGG CCAGGGTTTC CGCCCCCAAC ATATGTTATC 
CCCCCGCCTG TGGCATTTTC TATGGGCTCA GGTTACACCT TCCCAGCTGG TGTTTCTGTC 
CCAGGAACCT TTCTTCAGCC TACAGCTCAC TCTCCAGCAG GAAACCAGGT GCAAGCTGGG 
AAACAGTCCC ACATTCCTTA CAGCCAGCAA CGGCCCTCTG GACCAGGGCC AATGAACCAG 
GGACCTCAAC AATCACAGCC ACCTTCCCAG CAACCCCTTA CATCTTTACC AGCTCAGCCA 
ACAGCACAGT CTACAAGCCA GCTGCAGGTT CAAGCTCTAA CTCAGCAACA ACAATCCCCT 
ACAAAAGCTG TGCCGGCTTT GGGGAAAAGC CCGCCTCACC ACTCTGGATT CCAGCAGTAT 
CAACAGGCAG ATGCCTCCAA ACAGCTGTGG AATCCCCCTC AGGTTCAAGG CCCATTAGGG 
AAAATTATGC CTGTGAAACA GCCCTACTAC CTTCAGACCC AAGACCCCAT AAAACTGTTT 
GAGCCGTCAT TGCAACCTCC TGTAATGCAG CAGCAGCCTC TAGAAAAAAA AATGAAGCCT 
TTTCCCATGG AGCCATATAA CCATAATCCC TCAGAAGTCA AGGTCCCAGA ATTCTACTGG 
GATTCTTCCT ACAGCATGGC TGATAACAGA TCTGTAATGG CACAGCAAGC AAACATAGAC 
CGCAGGGGCA AACGGTCACC AGGAGTCTTC CGTCCAGAGC AGGATCCTGT ACCCAGAATG 
CCGTTTGAGA AATCCTTATT GGAGAAGCCC TCAGAGCTCA TGTCACATTC ATCCTCTTTC 
CTGTCCCTCA CCGGATTCTC TCTCAATCAG GAAAGATACC CAAATAATAG TATGTTCAAT 
GAGGTATATG GGAAAAACCT GACATCCAGC TCCAAAGCAG AACTCAGTCC CTCAATGGCC 
CCCCAGGAAA CATCTCTGTA TTCCCTTTTT GAAGGGACTC CGTGGTCTCC ATCACTTCCT 
GCCAGTTCAG ATCATTCAAC ACCAGCCAGC CAGTCTCCTC ATTCCTCTAA CCCAAGCAGC 
CTACCCAGCT CTCCTCCAAC ACACAACCAT AATTCTGTTC CATTCTCCAA TTTTGGACCC 
ATTGGGACTC CAGATAACAG GGATAGAAGG ACTGCAGATC GGTGGAAAAC TGATAAGCCA 
GCCATGGGTG GGTTTGGCAT TGATTATCTC TCAGCAACGT CATCCTCTGA GAGCAGTTGG 
CATCAGGCCA GCACTCCGAG TGGCACCTGG ACAGGCCATG GCCCTTCCAT GGAGGATTCC 
TCTGCTGTCC TCATGGAAAG CCTAAAGTCT ATCTGGTCCA GTTCCATGAT GCATCCTGGA 
CCTTCTGCTC TGGAGCAGCT GTTAATGCAG CAGAAGCAGA AACAGCAACG GGGACAAGGC 
ACCATGAACC CTCCACACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG7 ( XM_011510209.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011508511.1 Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X9, mRNA.

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CloneID	OHu63455
Clone ID Related Accession (Same CDS sequence)	NM_001350219.1 , NM_001350219.2 , XM_011510209.3 , XM_011510209.2
Accession Version	XM_011510209.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3363bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein SMG7 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011510209.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
CAGATGAGGA CCGAAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG 
GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCTGA AGTCTGGACA 
TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTAG TTACCGATTT GGAATACGCT 
TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCACGCCT TTAAGAATCA GATCACAACA 
CTGCAAGGCC AGGCAAAGAA TCGAGCAAAT CCGAATCGGA GTGAAGTTCA GGCAAACCTT 
TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA 
GTATTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AATTGGGAAT TATCAGCAAT 
AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC 
CAGCACTGCC TCGTCCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA 
GAGTCCTACT ATAGGCATGC AGCTCAGCTT GTCCCCTCCA ATGGTCAGCC TTATAATCAG 
TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACCA CAATTTTCTA CTACTGCAGA 
AGCATTGCTG TGAAGTTCCC TTTCCCAGCT GCCTCCACTA ATCTGCAAAA AGCACTTTCT 
AAAGCACTGG AAAGCCGAGA TGAGGTGAAA ACCAAGTGGG GTGTTTCTGA CTTCATCAAG 
GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT 
CTTCGAGAGA AATTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAAAAAGC TTTCAACTCT 
CAGCAGTTAG TTCATGTCAC TGTCATTAAC CTGTTTCAAC TTCATCACCT TCGTGACTTT 
AGCAATGAAA CCGAGCAGCA CACTTATAGC CAAGATGAGC AGCTATGTTG GACACAGTTG 
CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTACA GAATGAGTCT 
CAGGAGGAGT CCTACAATGC CTATCCTCTT CCAGCAGTCA AGGTCTCCAT GGACTGGCTA 
AGACTCAGAC CCAGGGTCTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCC 
TGGTTGATTT CTCTTCTGAA TAGTTTCCAT CCCCATGAAG AGGACCTCTC AAGTATTAGT 
GCGACACCAC TTCCAGAGGA GTTTGAATTA CAAGGATTTT TGGCATTGAG ACCTTCTTTC 
AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GGGACAAAGA AGGCCAGCAA 
CGACGAATAC GACAGCAACG CTTGATCTCT ATAGGCAAAT GGATTGCTGA TAATCAGCCA 
AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAA 
TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA AACATCTGTG 
ATAGAGTCGC TGGCTGCAGA TGGGAGCCCA GGGCTAAAAT CAGTGCTATC TACAAGCCGA 
AATTTAAGCA ACAACTGTGA CACAGGAGAG AAGCCAGTGG TTACCTTCAA AGAAAACATT 
AAGACACGAG AAGTGAACAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTAAAATCC 
CAGACAGAAC TAAGAAAGAC TCCAGTGTCT GAAGCCAGAA AAACACCTGT AACTCAAACC 
CCAACTCAAG CAAGTAACTC CCAGTTCATC CCCATTCATC ACCCTGGAGC CTTCCCTCCT 
CTTCCCAGCA GGCCAGGGTT TCCGCCCCCA ACATATGTTA TCCCCCCGCC TGTGGCATTT 
TCTATGGGCT CAGGTTACAC CTTCCCAGCT GGTGTTTCTG TCCCAGGAAC CTTTCTTCAG 
CCTACAGCTC ACTCTCCAGC AGGAAACCAG GTGCAAGCTG GGAAACAGTC CCACATTCCT 
TACAGCCAGC AACGGCCCTC TGGACCAGGG CCAATGAACC AGGGACCTCA ACAATCACAG 
CCACCTTCCC AGCAACCCCT TACATCTTTA CCAGCTCAGC CAACAGCACA GTCTACAAGC 
CAGCTGCAGG TTCAAGCTCT AACTCAGCAA CAACAATCCC CTACAAAAGC TGTGCCGGCT 
TTGGGGAAAA GCCCGCCTCA CCACTCTGGA TTCCAGCAGT ATCAACAGGC AGATGCCTCC 
AAACAGCTGT GGAATCCCCC TCAGGTTCAA GGCCCATTAG GGAAAATTAT GCCTGTGAAA 
CAGCCCTACT ACCTTCAGAC CCAAGACCCC ATAAAACTGT TTGAGCCGTC ATTGCAACCT 
CCTGTAATGC AGCAGCAGCC TCTAGAAAAA AAAATGAAGC CTTTTCCCAT GGAGCCATAT 
AACCATAATC CCTCAGAAGT CAAGGTCCCA GAATTCTACT GGGATTCTTC CTACAGCATG 
GCTGATAACA GATCTGTAAT GGCACAGCAA GCAAACATAG ACCGCAGGGG CAAACGGTCA 
CCAGGAGTCT TCCGTCCAGA GCAGGATCCT GTACCCAGAA TGCCGTTTGA GAAATCCTTA 
TTGGAGAAGC CCTCAGAGCT CATGTCACAT TCATCCTCTT TCCTGTCCCT CACCGGATTC 
TCTCTCAATC AGGAAAGATA CCCAAATAAT AGTATGTTCA ATGAGGTATA TGGGAAAAAC 
CTGACATCCA GCTCCAAAGC AGAACTCAGT CCCTCAATGG CCCCCCAGGA AACATCTCTG 
TATTCCCTTT TTGAAGGGAC TCCGTGGTCT CCATCACTTC CTGCCAGTTC AGATCATTCA 
ACACCAGCCA GCCAGTCTCC TCATTCCTCT AACCCAAGCA GCCTACCCAG CTCTCCTCCA 
ACACACAACC ATAATTCTGT TCCATTCTCC AATTTTGGAC CCATTGGGAC TCCAGATAAC 
AGGGATAGAA GGACTGCAGA TCGGTGGAAA ACTGATAAGC CAGCCATGGG TGGGTTTGGC 
ATTGATTATC TCTCAGCAAC GTCATCCTCT GAGAGCAGTT GGCATCAGGC CAGCACTCCG 
AGTGGCACCT GGACAGGCCA TGGCCCTTCC ATGGAGGATT CCTCTGCTGT CCTCATGGAA 
AGCCTAAAGT CTATCTGGTC CAGTTCCATG ATGCATCCTG GACCTTCTGC TCTGGAGCAG 
CTGTTAATGC AGCAGAAGCA GAAACAGCAA CGGGGACAAG GCACCATGAA CCCTCCACAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011508511.1
CDS	996..4358
Translation	MRNWMFLQIVIVGENVSFKSQMRTENLKSEEHLKSSNIRQAEVLKADMTDSKLGPAEVWTSRQALQDLYQKMLVTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRANPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQTHTSAIVKPQSSSCSYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNLQKALSKALESRDEVKTKWGVSDFIKAFIKFHGHVYLSKSLEKLSPLREKLEEQFKRLLFQKAFNSQQLVHVTVINLFQLHHLRDFSNETEQHTYSQDEQLCWTQLLALFMSFLGILCKCPLQNESQEESYNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPHEEDLSSISATPLPEEFELQGFLALRPSFRNLDFSKGHQGITGDKEGQQRRIRQQRLISIGKWIADNQPRLIQCENEVGKLLFITEIPELILEDPSEAKENLILQETSVIESLAADGSPGLKSVLSTSRNLSNNCDTGEKPVVTFKENIKTREVNRDQGRSFPPKEVKSQTELRKTPVSEARKTPVTQTPTQASNSQFIPIHHPGAFPPLPSRPGFPPPTYVIPPPVAFSMGSGYTFPAGVSVPGTFLQPTAHSPAGNQVQAGKQSHIPYSQQRPSGPGPMNQGPQQSQPPSQQPLTSLPAQPTAQSTSQLQVQALTQQQQSPTKAVPALGKSPPHHSGFQQYQQADASKQLWNPPQVQGPLGKIMPVKQPYYLQTQDPIKLFEPSLQPPVMQQQPLEKKMKPFPMEPYNHNPSEVKVPEFYWDSSYSMADNRSVMAQQANIDRRGKRSPGVFRPEQDPVPRMPFEKSLLEKPSELMSHSSSFLSLTGFSLNQERYPNNSMFNEVYGKNLTSSSKAELSPSMAPQETSLYSLFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSVPFSNFGPIGTPDNRDRRTADRWKTDKPAMGGFGIDYLSATSSSESSWHQASTPSGTWTGHGPSMEDSSAVLMESLKSIWSSSMMHPGPSALEQLLMQQKQKQQRGQGTMNPPH

Target ORF information:

RefSeq Version	XM_011510209.3
Organism	Homo sapiens(human)
Definition	Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011510209.3

ORF Insert Sequence:

ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
CAGATGAGGA CCGAAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG 
GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCTGA AGTCTGGACA 
TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTAG TTACCGATTT GGAATACGCT 
TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCACGCCT TTAAGAATCA GATCACAACA 
CTGCAAGGCC AGGCAAAGAA TCGAGCAAAT CCGAATCGGA GTGAAGTTCA GGCAAACCTT 
TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA 
GTATTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AATTGGGAAT TATCAGCAAT 
AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC 
CAGCACTGCC TCGTCCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA 
GAGTCCTACT ATAGGCATGC AGCTCAGCTT GTCCCCTCCA ATGGTCAGCC TTATAATCAG 
TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACCA CAATTTTCTA CTACTGCAGA 
AGCATTGCTG TGAAGTTCCC TTTCCCAGCT GCCTCCACTA ATCTGCAAAA AGCACTTTCT 
AAAGCACTGG AAAGCCGAGA TGAGGTGAAA ACCAAGTGGG GTGTTTCTGA CTTCATCAAG 
GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT 
CTTCGAGAGA AATTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAAAAAGC TTTCAACTCT 
CAGCAGTTAG TTCATGTCAC TGTCATTAAC CTGTTTCAAC TTCATCACCT TCGTGACTTT 
AGCAATGAAA CCGAGCAGCA CACTTATAGC CAAGATGAGC AGCTATGTTG GACACAGTTG 
CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTACA GAATGAGTCT 
CAGGAGGAGT CCTACAATGC CTATCCTCTT CCAGCAGTCA AGGTCTCCAT GGACTGGCTA 
AGACTCAGAC CCAGGGTCTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCC 
TGGTTGATTT CTCTTCTGAA TAGTTTCCAT CCCCATGAAG AGGACCTCTC AAGTATTAGT 
GCGACACCAC TTCCAGAGGA GTTTGAATTA CAAGGATTTT TGGCATTGAG ACCTTCTTTC 
AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GGGACAAAGA AGGCCAGCAA 
CGACGAATAC GACAGCAACG CTTGATCTCT ATAGGCAAAT GGATTGCTGA TAATCAGCCA 
AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAA 
TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA AACATCTGTG 
ATAGAGTCGC TGGCTGCAGA TGGGAGCCCA GGGCTAAAAT CAGTGCTATC TACAAGCCGA 
AATTTAAGCA ACAACTGTGA CACAGGAGAG AAGCCAGTGG TTACCTTCAA AGAAAACATT 
AAGACACGAG AAGTGAACAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTAAAATCC 
CAGACAGAAC TAAGAAAGAC TCCAGTGTCT GAAGCCAGAA AAACACCTGT AACTCAAACC 
CCAACTCAAG CAAGTAACTC CCAGTTCATC CCCATTCATC ACCCTGGAGC CTTCCCTCCT 
CTTCCCAGCA GGCCAGGGTT TCCGCCCCCA ACATATGTTA TCCCCCCGCC TGTGGCATTT 
TCTATGGGCT CAGGTTACAC CTTCCCAGCT GGTGTTTCTG TCCCAGGAAC CTTTCTTCAG 
CCTACAGCTC ACTCTCCAGC AGGAAACCAG GTGCAAGCTG GGAAACAGTC CCACATTCCT 
TACAGCCAGC AACGGCCCTC TGGACCAGGG CCAATGAACC AGGGACCTCA ACAATCACAG 
CCACCTTCCC AGCAACCCCT TACATCTTTA CCAGCTCAGC CAACAGCACA GTCTACAAGC 
CAGCTGCAGG TTCAAGCTCT AACTCAGCAA CAACAATCCC CTACAAAAGC TGTGCCGGCT 
TTGGGGAAAA GCCCGCCTCA CCACTCTGGA TTCCAGCAGT ATCAACAGGC AGATGCCTCC 
AAACAGCTGT GGAATCCCCC TCAGGTTCAA GGCCCATTAG GGAAAATTAT GCCTGTGAAA 
CAGCCCTACT ACCTTCAGAC CCAAGACCCC ATAAAACTGT TTGAGCCGTC ATTGCAACCT 
CCTGTAATGC AGCAGCAGCC TCTAGAAAAA AAAATGAAGC CTTTTCCCAT GGAGCCATAT 
AACCATAATC CCTCAGAAGT CAAGGTCCCA GAATTCTACT GGGATTCTTC CTACAGCATG 
GCTGATAACA GATCTGTAAT GGCACAGCAA GCAAACATAG ACCGCAGGGG CAAACGGTCA 
CCAGGAGTCT TCCGTCCAGA GCAGGATCCT GTACCCAGAA TGCCGTTTGA GAAATCCTTA 
TTGGAGAAGC CCTCAGAGCT CATGTCACAT TCATCCTCTT TCCTGTCCCT CACCGGATTC 
TCTCTCAATC AGGAAAGATA CCCAAATAAT AGTATGTTCA ATGAGGTATA TGGGAAAAAC 
CTGACATCCA GCTCCAAAGC AGAACTCAGT CCCTCAATGG CCCCCCAGGA AACATCTCTG 
TATTCCCTTT TTGAAGGGAC TCCGTGGTCT CCATCACTTC CTGCCAGTTC AGATCATTCA 
ACACCAGCCA GCCAGTCTCC TCATTCCTCT AACCCAAGCA GCCTACCCAG CTCTCCTCCA 
ACACACAACC ATAATTCTGT TCCATTCTCC AATTTTGGAC CCATTGGGAC TCCAGATAAC 
AGGGATAGAA GGACTGCAGA TCGGTGGAAA ACTGATAAGC CAGCCATGGG TGGGTTTGGC 
ATTGATTATC TCTCAGCAAC GTCATCCTCT GAGAGCAGTT GGCATCAGGC CAGCACTCCG 
AGTGGCACCT GGACAGGCCA TGGCCCTTCC ATGGAGGATT CCTCTGCTGT CCTCATGGAA 
AGCCTAAAGT CTATCTGGTC CAGTTCCATG ATGCATCCTG GACCTTCTGC TCTGGAGCAG 
CTGTTAATGC AGCAGAAGCA GAAACAGCAA CGGGGACAAG GCACCATGAA CCCTCCACAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG7 ( XM_011510209.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011508511.1 Homo sapiens SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X12, mRNA.

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CloneID	OHu63455
Clone ID Related Accession (Same CDS sequence)	NM_001350219.1 , NM_001350219.2 , XM_011510209.3 , XM_011510209.2
Accession Version	XM_011510209.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3363bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	protein SMG7 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011510209.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
CAGATGAGGA CCGAAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG 
GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCTGA AGTCTGGACA 
TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTAG TTACCGATTT GGAATACGCT 
TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCACGCCT TTAAGAATCA GATCACAACA 
CTGCAAGGCC AGGCAAAGAA TCGAGCAAAT CCGAATCGGA GTGAAGTTCA GGCAAACCTT 
TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA 
GTATTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AATTGGGAAT TATCAGCAAT 
AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC 
CAGCACTGCC TCGTCCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA 
GAGTCCTACT ATAGGCATGC AGCTCAGCTT GTCCCCTCCA ATGGTCAGCC TTATAATCAG 
TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACCA CAATTTTCTA CTACTGCAGA 
AGCATTGCTG TGAAGTTCCC TTTCCCAGCT GCCTCCACTA ATCTGCAAAA AGCACTTTCT 
AAAGCACTGG AAAGCCGAGA TGAGGTGAAA ACCAAGTGGG GTGTTTCTGA CTTCATCAAG 
GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT 
CTTCGAGAGA AATTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAAAAAGC TTTCAACTCT 
CAGCAGTTAG TTCATGTCAC TGTCATTAAC CTGTTTCAAC TTCATCACCT TCGTGACTTT 
AGCAATGAAA CCGAGCAGCA CACTTATAGC CAAGATGAGC AGCTATGTTG GACACAGTTG 
CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTACA GAATGAGTCT 
CAGGAGGAGT CCTACAATGC CTATCCTCTT CCAGCAGTCA AGGTCTCCAT GGACTGGCTA 
AGACTCAGAC CCAGGGTCTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCC 
TGGTTGATTT CTCTTCTGAA TAGTTTCCAT CCCCATGAAG AGGACCTCTC AAGTATTAGT 
GCGACACCAC TTCCAGAGGA GTTTGAATTA CAAGGATTTT TGGCATTGAG ACCTTCTTTC 
AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GGGACAAAGA AGGCCAGCAA 
CGACGAATAC GACAGCAACG CTTGATCTCT ATAGGCAAAT GGATTGCTGA TAATCAGCCA 
AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAA 
TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA AACATCTGTG 
ATAGAGTCGC TGGCTGCAGA TGGGAGCCCA GGGCTAAAAT CAGTGCTATC TACAAGCCGA 
AATTTAAGCA ACAACTGTGA CACAGGAGAG AAGCCAGTGG TTACCTTCAA AGAAAACATT 
AAGACACGAG AAGTGAACAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTAAAATCC 
CAGACAGAAC TAAGAAAGAC TCCAGTGTCT GAAGCCAGAA AAACACCTGT AACTCAAACC 
CCAACTCAAG CAAGTAACTC CCAGTTCATC CCCATTCATC ACCCTGGAGC CTTCCCTCCT 
CTTCCCAGCA GGCCAGGGTT TCCGCCCCCA ACATATGTTA TCCCCCCGCC TGTGGCATTT 
TCTATGGGCT CAGGTTACAC CTTCCCAGCT GGTGTTTCTG TCCCAGGAAC CTTTCTTCAG 
CCTACAGCTC ACTCTCCAGC AGGAAACCAG GTGCAAGCTG GGAAACAGTC CCACATTCCT 
TACAGCCAGC AACGGCCCTC TGGACCAGGG CCAATGAACC AGGGACCTCA ACAATCACAG 
CCACCTTCCC AGCAACCCCT TACATCTTTA CCAGCTCAGC CAACAGCACA GTCTACAAGC 
CAGCTGCAGG TTCAAGCTCT AACTCAGCAA CAACAATCCC CTACAAAAGC TGTGCCGGCT 
TTGGGGAAAA GCCCGCCTCA CCACTCTGGA TTCCAGCAGT ATCAACAGGC AGATGCCTCC 
AAACAGCTGT GGAATCCCCC TCAGGTTCAA GGCCCATTAG GGAAAATTAT GCCTGTGAAA 
CAGCCCTACT ACCTTCAGAC CCAAGACCCC ATAAAACTGT TTGAGCCGTC ATTGCAACCT 
CCTGTAATGC AGCAGCAGCC TCTAGAAAAA AAAATGAAGC CTTTTCCCAT GGAGCCATAT 
AACCATAATC CCTCAGAAGT CAAGGTCCCA GAATTCTACT GGGATTCTTC CTACAGCATG 
GCTGATAACA GATCTGTAAT GGCACAGCAA GCAAACATAG ACCGCAGGGG CAAACGGTCA 
CCAGGAGTCT TCCGTCCAGA GCAGGATCCT GTACCCAGAA TGCCGTTTGA GAAATCCTTA 
TTGGAGAAGC CCTCAGAGCT CATGTCACAT TCATCCTCTT TCCTGTCCCT CACCGGATTC 
TCTCTCAATC AGGAAAGATA CCCAAATAAT AGTATGTTCA ATGAGGTATA TGGGAAAAAC 
CTGACATCCA GCTCCAAAGC AGAACTCAGT CCCTCAATGG CCCCCCAGGA AACATCTCTG 
TATTCCCTTT TTGAAGGGAC TCCGTGGTCT CCATCACTTC CTGCCAGTTC AGATCATTCA 
ACACCAGCCA GCCAGTCTCC TCATTCCTCT AACCCAAGCA GCCTACCCAG CTCTCCTCCA 
ACACACAACC ATAATTCTGT TCCATTCTCC AATTTTGGAC CCATTGGGAC TCCAGATAAC 
AGGGATAGAA GGACTGCAGA TCGGTGGAAA ACTGATAAGC CAGCCATGGG TGGGTTTGGC 
ATTGATTATC TCTCAGCAAC GTCATCCTCT GAGAGCAGTT GGCATCAGGC CAGCACTCCG 
AGTGGCACCT GGACAGGCCA TGGCCCTTCC ATGGAGGATT CCTCTGCTGT CCTCATGGAA 
AGCCTAAAGT CTATCTGGTC CAGTTCCATG ATGCATCCTG GACCTTCTGC TCTGGAGCAG 
CTGTTAATGC AGCAGAAGCA GAAACAGCAA CGGGGACAAG GCACCATGAA CCCTCCACAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011508511.1
CDS	1027..4389
Translation	MRNWMFLQIVIVGENVSFKSQMRTENLKSEEHLKSSNIRQAEVLKADMTDSKLGPAEVWTSRQALQDLYQKMLVTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRANPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQTHTSAIVKPQSSSCSYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNLQKALSKALESRDEVKTKWGVSDFIKAFIKFHGHVYLSKSLEKLSPLREKLEEQFKRLLFQKAFNSQQLVHVTVINLFQLHHLRDFSNETEQHTYSQDEQLCWTQLLALFMSFLGILCKCPLQNESQEESYNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPHEEDLSSISATPLPEEFELQGFLALRPSFRNLDFSKGHQGITGDKEGQQRRIRQQRLISIGKWIADNQPRLIQCENEVGKLLFITEIPELILEDPSEAKENLILQETSVIESLAADGSPGLKSVLSTSRNLSNNCDTGEKPVVTFKENIKTREVNRDQGRSFPPKEVKSQTELRKTPVSEARKTPVTQTPTQASNSQFIPIHHPGAFPPLPSRPGFPPPTYVIPPPVAFSMGSGYTFPAGVSVPGTFLQPTAHSPAGNQVQAGKQSHIPYSQQRPSGPGPMNQGPQQSQPPSQQPLTSLPAQPTAQSTSQLQVQALTQQQQSPTKAVPALGKSPPHHSGFQQYQQADASKQLWNPPQVQGPLGKIMPVKQPYYLQTQDPIKLFEPSLQPPVMQQQPLEKKMKPFPMEPYNHNPSEVKVPEFYWDSSYSMADNRSVMAQQANIDRRGKRSPGVFRPEQDPVPRMPFEKSLLEKPSELMSHSSSFLSLTGFSLNQERYPNNSMFNEVYGKNLTSSSKAELSPSMAPQETSLYSLFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSVPFSNFGPIGTPDNRDRRTADRWKTDKPAMGGFGIDYLSATSSSESSWHQASTPSGTWTGHGPSMEDSSAVLMESLKSIWSSSMMHPGPSALEQLLMQQKQKQQRGQGTMNPPH

Target ORF information:

RefSeq Version	XM_011510209.2
Organism	Homo sapiens(human)
Definition	Homo sapiens SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011510209.2

ORF Insert Sequence:

ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
CAGATGAGGA CCGAAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG 
GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCTGA AGTCTGGACA 
TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTAG TTACCGATTT GGAATACGCT 
TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCACGCCT TTAAGAATCA GATCACAACA 
CTGCAAGGCC AGGCAAAGAA TCGAGCAAAT CCGAATCGGA GTGAAGTTCA GGCAAACCTT 
TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA 
GTATTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AATTGGGAAT TATCAGCAAT 
AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC 
CAGCACTGCC TCGTCCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA 
GAGTCCTACT ATAGGCATGC AGCTCAGCTT GTCCCCTCCA ATGGTCAGCC TTATAATCAG 
TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACCA CAATTTTCTA CTACTGCAGA 
AGCATTGCTG TGAAGTTCCC TTTCCCAGCT GCCTCCACTA ATCTGCAAAA AGCACTTTCT 
AAAGCACTGG AAAGCCGAGA TGAGGTGAAA ACCAAGTGGG GTGTTTCTGA CTTCATCAAG 
GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT 
CTTCGAGAGA AATTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAAAAAGC TTTCAACTCT 
CAGCAGTTAG TTCATGTCAC TGTCATTAAC CTGTTTCAAC TTCATCACCT TCGTGACTTT 
AGCAATGAAA CCGAGCAGCA CACTTATAGC CAAGATGAGC AGCTATGTTG GACACAGTTG 
CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTACA GAATGAGTCT 
CAGGAGGAGT CCTACAATGC CTATCCTCTT CCAGCAGTCA AGGTCTCCAT GGACTGGCTA 
AGACTCAGAC CCAGGGTCTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCC 
TGGTTGATTT CTCTTCTGAA TAGTTTCCAT CCCCATGAAG AGGACCTCTC AAGTATTAGT 
GCGACACCAC TTCCAGAGGA GTTTGAATTA CAAGGATTTT TGGCATTGAG ACCTTCTTTC 
AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GGGACAAAGA AGGCCAGCAA 
CGACGAATAC GACAGCAACG CTTGATCTCT ATAGGCAAAT GGATTGCTGA TAATCAGCCA 
AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAA 
TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA AACATCTGTG 
ATAGAGTCGC TGGCTGCAGA TGGGAGCCCA GGGCTAAAAT CAGTGCTATC TACAAGCCGA 
AATTTAAGCA ACAACTGTGA CACAGGAGAG AAGCCAGTGG TTACCTTCAA AGAAAACATT 
AAGACACGAG AAGTGAACAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTAAAATCC 
CAGACAGAAC TAAGAAAGAC TCCAGTGTCT GAAGCCAGAA AAACACCTGT AACTCAAACC 
CCAACTCAAG CAAGTAACTC CCAGTTCATC CCCATTCATC ACCCTGGAGC CTTCCCTCCT 
CTTCCCAGCA GGCCAGGGTT TCCGCCCCCA ACATATGTTA TCCCCCCGCC TGTGGCATTT 
TCTATGGGCT CAGGTTACAC CTTCCCAGCT GGTGTTTCTG TCCCAGGAAC CTTTCTTCAG 
CCTACAGCTC ACTCTCCAGC AGGAAACCAG GTGCAAGCTG GGAAACAGTC CCACATTCCT 
TACAGCCAGC AACGGCCCTC TGGACCAGGG CCAATGAACC AGGGACCTCA ACAATCACAG 
CCACCTTCCC AGCAACCCCT TACATCTTTA CCAGCTCAGC CAACAGCACA GTCTACAAGC 
CAGCTGCAGG TTCAAGCTCT AACTCAGCAA CAACAATCCC CTACAAAAGC TGTGCCGGCT 
TTGGGGAAAA GCCCGCCTCA CCACTCTGGA TTCCAGCAGT ATCAACAGGC AGATGCCTCC 
AAACAGCTGT GGAATCCCCC TCAGGTTCAA GGCCCATTAG GGAAAATTAT GCCTGTGAAA 
CAGCCCTACT ACCTTCAGAC CCAAGACCCC ATAAAACTGT TTGAGCCGTC ATTGCAACCT 
CCTGTAATGC AGCAGCAGCC TCTAGAAAAA AAAATGAAGC CTTTTCCCAT GGAGCCATAT 
AACCATAATC CCTCAGAAGT CAAGGTCCCA GAATTCTACT GGGATTCTTC CTACAGCATG 
GCTGATAACA GATCTGTAAT GGCACAGCAA GCAAACATAG ACCGCAGGGG CAAACGGTCA 
CCAGGAGTCT TCCGTCCAGA GCAGGATCCT GTACCCAGAA TGCCGTTTGA GAAATCCTTA 
TTGGAGAAGC CCTCAGAGCT CATGTCACAT TCATCCTCTT TCCTGTCCCT CACCGGATTC 
TCTCTCAATC AGGAAAGATA CCCAAATAAT AGTATGTTCA ATGAGGTATA TGGGAAAAAC 
CTGACATCCA GCTCCAAAGC AGAACTCAGT CCCTCAATGG CCCCCCAGGA AACATCTCTG 
TATTCCCTTT TTGAAGGGAC TCCGTGGTCT CCATCACTTC CTGCCAGTTC AGATCATTCA 
ACACCAGCCA GCCAGTCTCC TCATTCCTCT AACCCAAGCA GCCTACCCAG CTCTCCTCCA 
ACACACAACC ATAATTCTGT TCCATTCTCC AATTTTGGAC CCATTGGGAC TCCAGATAAC 
AGGGATAGAA GGACTGCAGA TCGGTGGAAA ACTGATAAGC CAGCCATGGG TGGGTTTGGC 
ATTGATTATC TCTCAGCAAC GTCATCCTCT GAGAGCAGTT GGCATCAGGC CAGCACTCCG 
AGTGGCACCT GGACAGGCCA TGGCCCTTCC ATGGAGGATT CCTCTGCTGT CCTCATGGAA 
AGCCTAAAGT CTATCTGGTC CAGTTCCATG ATGCATCCTG GACCTTCTGC TCTGGAGCAG 
CTGTTAATGC AGCAGAAGCA GAAACAGCAA CGGGGACAAG GCACCATGAA CCCTCCACAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG7 ( XM_011510210.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011508512.1 Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OHu63456
Clone ID Related Accession (Same CDS sequence)	XM_011510210.2
Accession Version	XM_011510210.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3300bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein SMG7 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011510210.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTAGTTA CCGATTTGGA ATACGCTTTA GACAAGAAAG TAGAACAGGA TCTCTGGAAT 
CACGCCTTTA AGAATCAGAT CACAACACTG CAAGGCCAGG CAAAGAATCG AGCAAATCCG 
AATCGGAGTG AAGTTCAGGC AAACCTTTCT CTGTTCCTAG AGGCAGCTAG TGGCTTCTAT 
ACTCAGTTAT TACAAGAACT GTGTACAGTA TTTAATGTAG ATTTACCATG CCGTGTGAAG 
TCTTCCCAAT TGGGAATTAT CAGCAATAAA CAGACGCATA CCAGCGCCAT AGTGAAGCCA 
CAGTCTAGCT CCTGTTCCTA TATCTGCCAG CACTGCCTCG TCCACCTTGG AGACATTGCT 
CGATACAGAA ACCAGACCAG CCAGGCAGAG TCCTACTATA GGCATGCAGC TCAGCTTGTC 
CCCTCCAATG GTCAGCCTTA TAATCAGTTG GCTATCTTAG CTTCTTCCAA AGGAGACCAT 
CTGACCACAA TTTTCTACTA CTGCAGAAGC ATTGCTGTGA AGTTCCCTTT CCCAGCTGCC 
TCCACTAATC TGCAAAAAGC ACTTTCTAAA GCACTGGAAA GCCGAGATGA GGTGAAAACC 
AAGTGGGGTG TTTCTGACTT CATCAAGGCC TTTATTAAAT TCCACGGTCA TGTGTACCTG 
AGTAAGAGCT TGGAAAAGTT GAGCCCTCTT CGAGAGAAAT TGGAAGAACA GTTTAAGAGG 
CTGCTATTCC AAAAAGCTTT CAACTCTCAG CAGTTAGTTC ATGTCACTGT CATTAACCTG 
TTTCAACTTC ATCACCTTCG TGACTTTAGC AATGAAACCG AGCAGCACAC TTATAGCCAA 
GATGAGCAGC TATGTTGGAC ACAGTTGCTG GCCCTCTTTA TGTCTTTTCT TGGCATCCTG 
TGCAAGTGTC CTCTACAGAA TGAGTCTCAG GAGGAGTCCT ACAATGCCTA TCCTCTTCCA 
GCAGTCAAGG TCTCCATGGA CTGGCTAAGA CTCAGACCCA GGGTCTTTCA GGAGGCAGTG 
GTGGATGAAA GACAGTACAT TTGGCCCTGG TTGATTTCTC TTCTGAATAG TTTCCATCCC 
CATGAAGAGG ACCTCTCAAG TATTAGTGCG ACACCACTTC CAGAGGAGTT TGAATTACAA 
GGATTTTTGG CATTGAGACC TTCTTTCAGG AACTTGGATT TTTCCAAAGG TCACCAGGGT 
ATTACAGGGG ACAAAGAAGG CCAGCAACGA CGAATACGAC AGCAACGCTT GATCTCTATA 
GGCAAATGGA TTGCTGATAA TCAGCCAAGG CTGATTCAGT GTGAAAATGA GGTAGGGAAA 
TTGTTGTTTA TCACAGAAAT CCCAGAATTA ATACTGGAAG ACCCCAGTGA AGCCAAAGAG 
AACCTCATTC TGCAAGAAAC ATCTGTGATA GAGTCGCTGG CTGCAGATGG GAGCCCAGGG 
CTAAAATCAG TGCTATCTAC AAGCCGAAAT TTAAGCAACA ACTGTGACAC AGGAGAGAAG 
CCAGTGGTTA CCTTCAAAGA AAACATTAAG ACACGAGAAG TGAACAGAGA CCAAGGAAGA 
AGTTTTCCTC CCAAAGAGGT AAAATCCCAG ACAGAACTAA GAAAGACTCC AGTGTCTGAA 
GCCAGAAAAA CACCTGTAAC TCAAACCCCA ACTCAAGCAA GTAACTCCCA GTTCATCCCC 
ATTCATCACC CTGGAGCCTT CCCTCCTCTT CCCAGCAGGC CAGGGTTTCC GCCCCCAACA 
TATGTTATCC CCCCGCCTGT GGCATTTTCT ATGGGCTCAG GTTACACCTT CCCAGCTGGT 
GTTTCTGTCC CAGGAACCTT TCTTCAGCCT ACAGCTCACT CTCCAGCAGG AAACCAGGTG 
CAAGCTGGGA AACAGTCCCA CATTCCTTAC AGCCAGCAAC GGCCCTCTGG ACCAGGGCCA 
ATGAACCAGG GACCTCAACA ATCACAGCCA CCTTCCCAGC AACCCCTTAC ATCTTTACCA 
GCTCAGCCAA CAGCACAGTC TACAAGCCAG CTGCAGGTTC AAGCTCTAAC TCAGCAACAA 
CAATCCCCTA CAAAAGCTGT GCCGGCTTTG GGGAAAAGCC CGCCTCACCA CTCTGGATTC 
CAGCAGTATC AACAGGCAGA TGCCTCCAAA CAGCTGTGGA ATCCCCCTCA GGTTCAAGGC 
CCATTAGGGA AAATTATGCC TGTGAAACAG CCCTACTACC TTCAGACCCA AGACCCCATA 
AAACTGTTTG AGCCGTCATT GCAACCTCCT GTAATGCAGC AGCAGCCTCT AGAAAAAAAA 
ATGAAGCCTT TTCCCATGGA GCCATATAAC CATAATCCCT CAGAAGTCAA GGTCCCAGAA 
TTCTACTGGG ATTCTTCCTA CAGCATGGCT GATAACAGAT CTGTAATGGC ACAGCAAGCA 
AACATAGACC GCAGGGGCAA ACGGTCACCA GGAGTCTTCC GTCCAGAGCA GGATCCTGTA 
CCCAGAATGC CGTTTGAGGA CCCCAAGAGC TCCCCTCTGC TTCCTCCGGA CCTGTTAAAG 
AGTCTGGCTG CCTTGGAGGA AGAGGAAGAG CTGATTTTTT CTAACCCTCC TGATCTTTAC 
CCGGCTCTGC TGGGGCCTCT CGCCTCTCTT CCTGGACGAA GCCTTTTTAA ATCCTTATTG 
GAGAAGCCCT CAGAGCTCAT GTCACATTCA TCCTCTTTCC TGTCCCTCAC CGGATTCTCT 
CTCAATCAGG AAAGATACCC AAATAATAGT ATGTTCAATG AGGTATATGG GAAAAACCTG 
ACATCCAGCT CCAAAGCAGA ACTCAGTCCC TCAATGGCCC CCCAGGAAAC ATCTCTGTAT 
TCCCTTTTTG AAGGGACTCC GTGGTCTCCA TCACTTCCTG CCAGTTCAGA TCATTCAACA 
CCAGCCAGCC AGTCTCCTCA TTCCTCTAAC CCAAGCAGCC TACCCAGCTC TCCTCCAACA 
CACAACCATA ATTCTGTTCC ATTCTCCAAT TTTGGACCCA TTGGGACTCC AGATAACAGG 
GATAGAAGGA CTGCAGATCG GTGGAAAACT GATAAGCCAG CCATGGGTGG GTTTGGCATT 
GATTATCTCT CAGCAACGTC ATCCTCTGAG AGCAGTTGGC ATCAGGCCAG CACTCCGAGT 
GGCACCTGGA CAGGCCATGG CCCTTCCATG GAGGATTCCT CTGCTGTCCT CATGGAAAGC 
CTAAAGTCTA TCTGGTCCAG TTCCATGATG CATCCTGGAC CTTCTGCTCT GGAGCAGCTG 
TTAATGCAGC AGAAGCAGAA ACAGCAACGG GGACAAGGCA CCATGAACCC TCCACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011508512.1
CDS	216..3515
Translation	MLVTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRANPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQTHTSAIVKPQSSSCSYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNLQKALSKALESRDEVKTKWGVSDFIKAFIKFHGHVYLSKSLEKLSPLREKLEEQFKRLLFQKAFNSQQLVHVTVINLFQLHHLRDFSNETEQHTYSQDEQLCWTQLLALFMSFLGILCKCPLQNESQEESYNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPHEEDLSSISATPLPEEFELQGFLALRPSFRNLDFSKGHQGITGDKEGQQRRIRQQRLISIGKWIADNQPRLIQCENEVGKLLFITEIPELILEDPSEAKENLILQETSVIESLAADGSPGLKSVLSTSRNLSNNCDTGEKPVVTFKENIKTREVNRDQGRSFPPKEVKSQTELRKTPVSEARKTPVTQTPTQASNSQFIPIHHPGAFPPLPSRPGFPPPTYVIPPPVAFSMGSGYTFPAGVSVPGTFLQPTAHSPAGNQVQAGKQSHIPYSQQRPSGPGPMNQGPQQSQPPSQQPLTSLPAQPTAQSTSQLQVQALTQQQQSPTKAVPALGKSPPHHSGFQQYQQADASKQLWNPPQVQGPLGKIMPVKQPYYLQTQDPIKLFEPSLQPPVMQQQPLEKKMKPFPMEPYNHNPSEVKVPEFYWDSSYSMADNRSVMAQQANIDRRGKRSPGVFRPEQDPVPRMPFEDPKSSPLLPPDLLKSLAALEEEEELIFSNPPDLYPALLGPLASLPGRSLFKSLLEKPSELMSHSSSFLSLTGFSLNQERYPNNSMFNEVYGKNLTSSSKAELSPSMAPQETSLYSLFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSVPFSNFGPIGTPDNRDRRTADRWKTDKPAMGGFGIDYLSATSSSESSWHQASTPSGTWTGHGPSMEDSSAVLMESLKSIWSSSMMHPGPSALEQLLMQQKQKQQRGQGTMNPPH

Target ORF information:

RefSeq Version	XM_011510210.2
Organism	Homo sapiens(human)
Definition	Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011510210.2

ORF Insert Sequence:

ATGCTAGTTA CCGATTTGGA ATACGCTTTA GACAAGAAAG TAGAACAGGA TCTCTGGAAT 
CACGCCTTTA AGAATCAGAT CACAACACTG CAAGGCCAGG CAAAGAATCG AGCAAATCCG 
AATCGGAGTG AAGTTCAGGC AAACCTTTCT CTGTTCCTAG AGGCAGCTAG TGGCTTCTAT 
ACTCAGTTAT TACAAGAACT GTGTACAGTA TTTAATGTAG ATTTACCATG CCGTGTGAAG 
TCTTCCCAAT TGGGAATTAT CAGCAATAAA CAGACGCATA CCAGCGCCAT AGTGAAGCCA 
CAGTCTAGCT CCTGTTCCTA TATCTGCCAG CACTGCCTCG TCCACCTTGG AGACATTGCT 
CGATACAGAA ACCAGACCAG CCAGGCAGAG TCCTACTATA GGCATGCAGC TCAGCTTGTC 
CCCTCCAATG GTCAGCCTTA TAATCAGTTG GCTATCTTAG CTTCTTCCAA AGGAGACCAT 
CTGACCACAA TTTTCTACTA CTGCAGAAGC ATTGCTGTGA AGTTCCCTTT CCCAGCTGCC 
TCCACTAATC TGCAAAAAGC ACTTTCTAAA GCACTGGAAA GCCGAGATGA GGTGAAAACC 
AAGTGGGGTG TTTCTGACTT CATCAAGGCC TTTATTAAAT TCCACGGTCA TGTGTACCTG 
AGTAAGAGCT TGGAAAAGTT GAGCCCTCTT CGAGAGAAAT TGGAAGAACA GTTTAAGAGG 
CTGCTATTCC AAAAAGCTTT CAACTCTCAG CAGTTAGTTC ATGTCACTGT CATTAACCTG 
TTTCAACTTC ATCACCTTCG TGACTTTAGC AATGAAACCG AGCAGCACAC TTATAGCCAA 
GATGAGCAGC TATGTTGGAC ACAGTTGCTG GCCCTCTTTA TGTCTTTTCT TGGCATCCTG 
TGCAAGTGTC CTCTACAGAA TGAGTCTCAG GAGGAGTCCT ACAATGCCTA TCCTCTTCCA 
GCAGTCAAGG TCTCCATGGA CTGGCTAAGA CTCAGACCCA GGGTCTTTCA GGAGGCAGTG 
GTGGATGAAA GACAGTACAT TTGGCCCTGG TTGATTTCTC TTCTGAATAG TTTCCATCCC 
CATGAAGAGG ACCTCTCAAG TATTAGTGCG ACACCACTTC CAGAGGAGTT TGAATTACAA 
GGATTTTTGG CATTGAGACC TTCTTTCAGG AACTTGGATT TTTCCAAAGG TCACCAGGGT 
ATTACAGGGG ACAAAGAAGG CCAGCAACGA CGAATACGAC AGCAACGCTT GATCTCTATA 
GGCAAATGGA TTGCTGATAA TCAGCCAAGG CTGATTCAGT GTGAAAATGA GGTAGGGAAA 
TTGTTGTTTA TCACAGAAAT CCCAGAATTA ATACTGGAAG ACCCCAGTGA AGCCAAAGAG 
AACCTCATTC TGCAAGAAAC ATCTGTGATA GAGTCGCTGG CTGCAGATGG GAGCCCAGGG 
CTAAAATCAG TGCTATCTAC AAGCCGAAAT TTAAGCAACA ACTGTGACAC AGGAGAGAAG 
CCAGTGGTTA CCTTCAAAGA AAACATTAAG ACACGAGAAG TGAACAGAGA CCAAGGAAGA 
AGTTTTCCTC CCAAAGAGGT AAAATCCCAG ACAGAACTAA GAAAGACTCC AGTGTCTGAA 
GCCAGAAAAA CACCTGTAAC TCAAACCCCA ACTCAAGCAA GTAACTCCCA GTTCATCCCC 
ATTCATCACC CTGGAGCCTT CCCTCCTCTT CCCAGCAGGC CAGGGTTTCC GCCCCCAACA 
TATGTTATCC CCCCGCCTGT GGCATTTTCT ATGGGCTCAG GTTACACCTT CCCAGCTGGT 
GTTTCTGTCC CAGGAACCTT TCTTCAGCCT ACAGCTCACT CTCCAGCAGG AAACCAGGTG 
CAAGCTGGGA AACAGTCCCA CATTCCTTAC AGCCAGCAAC GGCCCTCTGG ACCAGGGCCA 
ATGAACCAGG GACCTCAACA ATCACAGCCA CCTTCCCAGC AACCCCTTAC ATCTTTACCA 
GCTCAGCCAA CAGCACAGTC TACAAGCCAG CTGCAGGTTC AAGCTCTAAC TCAGCAACAA 
CAATCCCCTA CAAAAGCTGT GCCGGCTTTG GGGAAAAGCC CGCCTCACCA CTCTGGATTC 
CAGCAGTATC AACAGGCAGA TGCCTCCAAA CAGCTGTGGA ATCCCCCTCA GGTTCAAGGC 
CCATTAGGGA AAATTATGCC TGTGAAACAG CCCTACTACC TTCAGACCCA AGACCCCATA 
AAACTGTTTG AGCCGTCATT GCAACCTCCT GTAATGCAGC AGCAGCCTCT AGAAAAAAAA 
ATGAAGCCTT TTCCCATGGA GCCATATAAC CATAATCCCT CAGAAGTCAA GGTCCCAGAA 
TTCTACTGGG ATTCTTCCTA CAGCATGGCT GATAACAGAT CTGTAATGGC ACAGCAAGCA 
AACATAGACC GCAGGGGCAA ACGGTCACCA GGAGTCTTCC GTCCAGAGCA GGATCCTGTA 
CCCAGAATGC CGTTTGAGGA CCCCAAGAGC TCCCCTCTGC TTCCTCCGGA CCTGTTAAAG 
AGTCTGGCTG CCTTGGAGGA AGAGGAAGAG CTGATTTTTT CTAACCCTCC TGATCTTTAC 
CCGGCTCTGC TGGGGCCTCT CGCCTCTCTT CCTGGACGAA GCCTTTTTAA ATCCTTATTG 
GAGAAGCCCT CAGAGCTCAT GTCACATTCA TCCTCTTTCC TGTCCCTCAC CGGATTCTCT 
CTCAATCAGG AAAGATACCC AAATAATAGT ATGTTCAATG AGGTATATGG GAAAAACCTG 
ACATCCAGCT CCAAAGCAGA ACTCAGTCCC TCAATGGCCC CCCAGGAAAC ATCTCTGTAT 
TCCCTTTTTG AAGGGACTCC GTGGTCTCCA TCACTTCCTG CCAGTTCAGA TCATTCAACA 
CCAGCCAGCC AGTCTCCTCA TTCCTCTAAC CCAAGCAGCC TACCCAGCTC TCCTCCAACA 
CACAACCATA ATTCTGTTCC ATTCTCCAAT TTTGGACCCA TTGGGACTCC AGATAACAGG 
GATAGAAGGA CTGCAGATCG GTGGAAAACT GATAAGCCAG CCATGGGTGG GTTTGGCATT 
GATTATCTCT CAGCAACGTC ATCCTCTGAG AGCAGTTGGC ATCAGGCCAG CACTCCGAGT 
GGCACCTGGA CAGGCCATGG CCCTTCCATG GAGGATTCCT CTGCTGTCCT CATGGAAAGC 
CTAAAGTCTA TCTGGTCCAG TTCCATGATG CATCCTGGAC CTTCTGCTCT GGAGCAGCTG 
TTAATGCAGC AGAAGCAGAA ACAGCAACGG GGACAAGGCA CCATGAACCC TCCACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Transcript-specific characteristics determine the contribution of endo- and exonucleolytic decay pathways during the degradation of nonsense-mediated decay substrates.
RNA (New York, N.Y.)23(8)1224-1236(2017 08)
Ottens F,Boehm V,Sibley CR,Ule J,Gehring NH

Transcriptome-wide identification of NMD-targeted human mRNAs reveals extensive redundancy between SMG6- and SMG7-mediated degradation pathways.
RNA (New York, N.Y.)23(2)189-201(2017 02)
Colombo M,Karousis ED,Bourquin J,Bruggmann R,M?hlemann O

Frameshift Mutations of SMG7 Essential for Nonsense-Mediated mRNA Decay in Gastric and Colorectal Cancers.
Pathology oncology research : POR23(1)221-222(2017 Jan)
Jo YS,Song SY,Kim MS,Yoo NJ,Lee SH

Decreased SMG7 expression associates with lupus-risk variants and elevated antinuclear antibody production.
Annals of the rheumatic diseases75(11)2007-2013(2016 Nov)
Deng Y,Zhao J,Sakurai D,Sestak AL,Osadchiy V,Langefeld CD,Kaufman KM,Kelly JA,James JA,Petri MA,Bae SC,Alarc?n-Riquelme ME,Alarc?n GS,Anaya JM,Criswell LA,Freedman BI,Kamen DL,Gilkeson GS,Jacob CO,Merrill JT,Gaffney PM,Sivils KM,Niewold TB,Ramsey-Goldman R,Reveille JD,Scofield RH,Stevens AM,Boackle SA,Vil? LM,Sohn II,Lee S,Chang DM,Song YW,Vyse TJ,Harley JB,Brown EE,Edberg JC,Kimberly RP,Cantor RM,Hahn BH,Grossman JM,Tsao BP

The host nonsense-mediated mRNA decay pathway restricts Mammalian RNA virus replication.
Cell host & microbe16(3)403-11(2014 Sep)
Balistreri G,Horvath P,Schweingruber C,Z?nd D,McInerney G,Merits A,M?hlemann O,Azzalin C,Helenius A

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SMG7 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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