Smg7 gene cDNA ORF clone, Mus musculus(house mouse)

mRNA	Protein	Name
NM_001160257.1	NP_001153729.1	protein SMG7 isoform 1
NM_001160257.2	NP_001153729.1	protein SMG7 isoform 1
XM_006529450.4	XP_006529513.1	protein SMG7 isoform X1
NM_001160256.1	NP_001153728.1	protein SMG7 isoform 2
NM_001359257.1	NP_001346186.1	protein SMG7 isoform 4
XM_006529452.3	XP_006529515.1	protein SMG7 isoform X3
XM_006529451.3	XP_006529514.1	protein SMG7 isoform X2
XM_006529451.4	XP_006529514.1	protein SMG7 isoform X2
XM_030253292.1	XP_030109152.1	protein SMG7 isoform X4
XM_030253294.1	XP_030109154.1	protein SMG7 isoform X5
NM_001005507.2	NP_001005507.1	protein SMG7 isoform 3
XM_017319978.1	XP_017175467.1	protein SMG7 isoform X4
XM_017319979.1	XP_017175468.1	protein SMG7 isoform X5
XM_030253296.1	XP_030109156.1	protein SMG7 isoform X6
XM_006529450.3	XP_006529513.1	protein SMG7 isoform X1
XM_017319981.1	XP_017175470.1	protein SMG7 isoform X6
XM_030253290.1	XP_030109150.1	protein SMG7 isoform X3
XM_030253298.1	XP_030109158.1	protein SMG7 isoform X6

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu77091	NM_001359257.1 Latest version!	Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$391.30 $559.00
OMu77091	XM_017319981.1 Latest version!	Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$391.30 $559.00
OMu85124	XM_030253296.1 Latest version!	Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu85124	XM_030253298.1 Latest version!	Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu08004	NM_001160256.1 Latest version!	Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$559.30 $799.00
OMu10690	NM_001005507.2 Latest version!	Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$559.30 $799.00
OMu28138	XM_006529450.4 Latest version!	Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu28139	XM_006529451.4 Latest version!	Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu28140	XM_030253290.1 Latest version!	Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu28141	XM_030253292.1 Latest version!	Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu85123	XM_030253294.1 Latest version!	Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu08009	NM_001160257.2 Latest version!	Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu08009	XM_006529452.3 Latest version!	Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu28140	XM_017319978.1 Latest version!	Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu28141	XM_017319979.1 Latest version!	Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
View Old Accession Versions >>
OMu08009	NM_001160257.1	Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu28139	XM_006529451.3	Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu28138	XM_006529450.3	Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
Hide Old Accession Versions >>

Smg7 ( NM_001359257.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001346186.1 Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OMu77091
Clone ID Related Accession (Same CDS sequence)	NM_001359257.1 , XM_017319981.1
Accession Version	NM_001359257.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2412bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-22
Organism	Mus musculus(house mouse)
Product	protein SMG7 isoform 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC110038.25 and AC123613.16. On Dec 29, 2017 this sequence version replaced XM_017319981.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660817.199105.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164141 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTTTTC TCGGCATCCT GTGCAAATGT CCTCTCCAGA ATGATTCTCA GGAGTCCAAC 
AATGCCTACC CCCTTCCTGC AGTCAAGGTC TCCATGGACT GGCTAAGACT CCGACCTAGA 
GTCTTTCAAG AAGCAGTGGT GGATGAAAGA CAGTACATTT GGCCCTGGCT AATTTCTCTT 
CTAAATAGTT TCCATCCCCG TGAAGATGAT CTCTCAAATA CTAATGCCAC ACCACTTCCA 
GAGGAGTTTG AATTACAAGG ATTCTTGGCT TTAAGACCTT CTTTCAGGAA CTTGGATTTT 
TCCAAAGGCC ATCAGGGTAT TACAGGAGAC AAAGAGGGTC AACAACGACG AATACGACAG 
CAGCGTTTGA TCTCCATAGG GAAATGGATT GCTGATAACC AGCCACGGCT GATTCAGTGT 
GAAAATGAGG TAGGGAAATT GTTATTTATC ACAGAAATCC CAGAATTAAT ACTAGAAGAC 
CCCAGTGAAG CCAAAGAGAA CCTCATCCTA CAAGAAACAT CTGTGGTAGA GTCACTGGCT 
ACGGATGGGA GCCCAGGACT GAAATCAGTG CTGTCTACAG GCCGAAATCC AAGCAACAGC 
TGTGACTCAG GAGAGAAACC AGTGGTCACC TTTAAAGAGA ACATTAAGCC ACGAGAAGTG 
AACCAAGGAA GAAGCTTTCC TCCCAAAGAG GTAAAATCCC AGACAGAACT AAGAAAGACT 
CCAGTGTCTG AAGCCAGGAA AACGCCTGTC ACTCAAACTC CAAGTCAAAC GAGTAATTCT 
CAGTTCATCC CCATCCATCA CCCTGGAGCC TTCCCTCCTC TTCCCAGCCG ACCAGGATTC 
CCGCCCCCAA CATATGTTAT CCCCCCTCCT GTGGCATTTT CTATGGGCTC AGGTTACACC 
TTCCCAGCTG GTGTTTCTGT CCCAGGAACC TTTCTTCAGT CTACAGCTCA CTCTCCAGCA 
GGAAACCAGG TGCAAGCTGG GAAACAGTCC CACATTCCTT ACAGCCAGCA ACGGCCCTCT 
GGACCAGGGC CAATGAACCA GGGACCTCAA CAGTCACAGC CACCTTCCCA GCCACCCCTT 
ACATCTTTAC CAGCTCAGCC AACAGCACAG TCTACAAGCC AATTGCAGGT TCAAGCTCTA 
GCTCAGCAAC AGCAATCCCC TACAAAAGTC ATACCAGCTT TGGGGAAAAG CCCGCCTCAC 
CACTCTGGAT TCCAGCAGTA TCAACAGGCA GATGCCTCCA AACAGCTGTG GAATCCCCCT 
CAGGTTCAAA GCCCACTAGG GAAAATTATG CCTGTGAAAC AGTCCTACTA CCTTCAGACC 
CAAGACCCTA TAAAACTGTT TGAGCCGTCA TTGCAACCTC CTGTAATACA GCAGCAGCCT 
CTAGAGAAAA AAATGAAGCC TTTCCCCATG GAGCCATATA ACCATAATCC CTCAGAAGTC 
AAGGTCCCAG AGTTCTACTG GGATTCTTCC TACAGCATGG CCGATAACAG AGCAGTAATG 
GCTCAGCAAC CAAATATGGA CCGCAGGAGC AAACGGTCAC CTGGAGTCTT CCGTCCAGAA 
CAGGATCCTG TGCCCAGGAT GCCATTTGAG GACCCCAAGA GCTCCCCTCT GCTTCCTCCG 
GACCTGTTAA AGAGTCTGGC TGCCTTGGAG GAAGAGGAAG AGCTGATCTT TTCTAACCCT 
CCTGATCTTT ACCCAGCTCT GCTGGGTCCT CTCGCCTCTC TTCCTGGACG AAGCCTCTTT 
AAATCCTTAT TGGAGAAGCC TTCTGAGCTC ATGTCACATT CATCTTCTTT CCTGTCCCTC 
ACGGGGTTCT CTGTCAATCA GGAAAGATAT CCAAACAGCA GTATGTTCAA TGAAGTGTAT 
GGAAAAAACC TGACAACCAG CTCCAAGGCA GAACTGAACC CCTCAGTCGC CTCCCAGGAA 
ACATCACTGT ACTCCCTTTT TGAAGGGACC CCATGGTCTC CATCACTTCC TGCCAGTTCA 
GATCATTCGA CACCAGCCAG CCAGTCTCCT CATTCCTCTA ACCCAAGCAG CCTGCCCAGT 
TCTCCTCCAA CGCATAACCA TAATTCTGCT CCATTCTCTA ATTTTGGACC CATTGGGACT 
CCAGATAACA GGGATAGGCG GCCTGCAGAT AGGTGGAAAA CTGATAAACC AGCCATGGGT 
GGTTTTGGTG TTGATTATCT CTCAGCAACG TCATCATCTG AGAGCAGTTG GCATCAGGCT 
AGCACTCCGA GTGGCACCTG GACAGGCCAC GGCCCCTCCA TGGAGGATTC CTCTGCTGTC 
CTCATGGAAA GCCTAAAGTC TATCTGGTCC AGTTCCATGA TGCATCCTGG GCCCTCCGCT 
CTGGAGCAGT TGTTAATGCA GCAGAAGCAG AAACAGCAGC GGGGACAAGG TGCCATGAAC 
CCTCCACACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001346186.1
CDS	1217..3628
Translation	MSFLGILCKCPLQNDSQESNNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPREDDLSNTNATPLPEEFELQGFLALRPSFRNLDFSKGHQGITGDKEGQQRRIRQQRLISIGKWIADNQPRLIQCENEVGKLLFITEIPELILEDPSEAKENLILQETSVVESLATDGSPGLKSVLSTGRNPSNSCDSGEKPVVTFKENIKPREVNQGRSFPPKEVKSQTELRKTPVSEARKTPVTQTPSQTSNSQFIPIHHPGAFPPLPSRPGFPPPTYVIPPPVAFSMGSGYTFPAGVSVPGTFLQSTAHSPAGNQVQAGKQSHIPYSQQRPSGPGPMNQGPQQSQPPSQPPLTSLPAQPTAQSTSQLQVQALAQQQQSPTKVIPALGKSPPHHSGFQQYQQADASKQLWNPPQVQSPLGKIMPVKQSYYLQTQDPIKLFEPSLQPPVIQQQPLEKKMKPFPMEPYNHNPSEVKVPEFYWDSSYSMADNRAVMAQQPNMDRRSKRSPGVFRPEQDPVPRMPFEDPKSSPLLPPDLLKSLAALEEEEELIFSNPPDLYPALLGPLASLPGRSLFKSLLEKPSELMSHSSSFLSLTGFSVNQERYPNSSMFNEVYGKNLTTSSKAELNPSVASQETSLYSLFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSAPFSNFGPIGTPDNRDRRPADRWKTDKPAMGGFGVDYLSATSSSESSWHQASTPSGTWTGHGPSMEDSSAVLMESLKSIWSSSMMHPGPSALEQLLMQQKQKQQRGQGAMNPPH

Target ORF information:

RefSeq Version	NM_001359257.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001359257.1

ORF Insert Sequence:

ATGTCTTTTC TCGGCATCCT GTGCAAATGT CCTCTCCAGA ATGATTCTCA GGAGTCCAAC 
AATGCCTACC CCCTTCCTGC AGTCAAGGTC TCCATGGACT GGCTAAGACT CCGACCTAGA 
GTCTTTCAAG AAGCAGTGGT GGATGAAAGA CAGTACATTT GGCCCTGGCT AATTTCTCTT 
CTAAATAGTT TCCATCCCCG TGAAGATGAT CTCTCAAATA CTAATGCCAC ACCACTTCCA 
GAGGAGTTTG AATTACAAGG ATTCTTGGCT TTAAGACCTT CTTTCAGGAA CTTGGATTTT 
TCCAAAGGCC ATCAGGGTAT TACAGGAGAC AAAGAGGGTC AACAACGACG AATACGACAG 
CAGCGTTTGA TCTCCATAGG GAAATGGATT GCTGATAACC AGCCACGGCT GATTCAGTGT 
GAAAATGAGG TAGGGAAATT GTTATTTATC ACAGAAATCC CAGAATTAAT ACTAGAAGAC 
CCCAGTGAAG CCAAAGAGAA CCTCATCCTA CAAGAAACAT CTGTGGTAGA GTCACTGGCT 
ACGGATGGGA GCCCAGGACT GAAATCAGTG CTGTCTACAG GCCGAAATCC AAGCAACAGC 
TGTGACTCAG GAGAGAAACC AGTGGTCACC TTTAAAGAGA ACATTAAGCC ACGAGAAGTG 
AACCAAGGAA GAAGCTTTCC TCCCAAAGAG GTAAAATCCC AGACAGAACT AAGAAAGACT 
CCAGTGTCTG AAGCCAGGAA AACGCCTGTC ACTCAAACTC CAAGTCAAAC GAGTAATTCT 
CAGTTCATCC CCATCCATCA CCCTGGAGCC TTCCCTCCTC TTCCCAGCCG ACCAGGATTC 
CCGCCCCCAA CATATGTTAT CCCCCCTCCT GTGGCATTTT CTATGGGCTC AGGTTACACC 
TTCCCAGCTG GTGTTTCTGT CCCAGGAACC TTTCTTCAGT CTACAGCTCA CTCTCCAGCA 
GGAAACCAGG TGCAAGCTGG GAAACAGTCC CACATTCCTT ACAGCCAGCA ACGGCCCTCT 
GGACCAGGGC CAATGAACCA GGGACCTCAA CAGTCACAGC CACCTTCCCA GCCACCCCTT 
ACATCTTTAC CAGCTCAGCC AACAGCACAG TCTACAAGCC AATTGCAGGT TCAAGCTCTA 
GCTCAGCAAC AGCAATCCCC TACAAAAGTC ATACCAGCTT TGGGGAAAAG CCCGCCTCAC 
CACTCTGGAT TCCAGCAGTA TCAACAGGCA GATGCCTCCA AACAGCTGTG GAATCCCCCT 
CAGGTTCAAA GCCCACTAGG GAAAATTATG CCTGTGAAAC AGTCCTACTA CCTTCAGACC 
CAAGACCCTA TAAAACTGTT TGAGCCGTCA TTGCAACCTC CTGTAATACA GCAGCAGCCT 
CTAGAGAAAA AAATGAAGCC TTTCCCCATG GAGCCATATA ACCATAATCC CTCAGAAGTC 
AAGGTCCCAG AGTTCTACTG GGATTCTTCC TACAGCATGG CCGATAACAG AGCAGTAATG 
GCTCAGCAAC CAAATATGGA CCGCAGGAGC AAACGGTCAC CTGGAGTCTT CCGTCCAGAA 
CAGGATCCTG TGCCCAGGAT GCCATTTGAG GACCCCAAGA GCTCCCCTCT GCTTCCTCCG 
GACCTGTTAA AGAGTCTGGC TGCCTTGGAG GAAGAGGAAG AGCTGATCTT TTCTAACCCT 
CCTGATCTTT ACCCAGCTCT GCTGGGTCCT CTCGCCTCTC TTCCTGGACG AAGCCTCTTT 
AAATCCTTAT TGGAGAAGCC TTCTGAGCTC ATGTCACATT CATCTTCTTT CCTGTCCCTC 
ACGGGGTTCT CTGTCAATCA GGAAAGATAT CCAAACAGCA GTATGTTCAA TGAAGTGTAT 
GGAAAAAACC TGACAACCAG CTCCAAGGCA GAACTGAACC CCTCAGTCGC CTCCCAGGAA 
ACATCACTGT ACTCCCTTTT TGAAGGGACC CCATGGTCTC CATCACTTCC TGCCAGTTCA 
GATCATTCGA CACCAGCCAG CCAGTCTCCT CATTCCTCTA ACCCAAGCAG CCTGCCCAGT 
TCTCCTCCAA CGCATAACCA TAATTCTGCT CCATTCTCTA ATTTTGGACC CATTGGGACT 
CCAGATAACA GGGATAGGCG GCCTGCAGAT AGGTGGAAAA CTGATAAACC AGCCATGGGT 
GGTTTTGGTG TTGATTATCT CTCAGCAACG TCATCATCTG AGAGCAGTTG GCATCAGGCT 
AGCACTCCGA GTGGCACCTG GACAGGCCAC GGCCCCTCCA TGGAGGATTC CTCTGCTGTC 
CTCATGGAAA GCCTAAAGTC TATCTGGTCC AGTTCCATGA TGCATCCTGG GCCCTCCGCT 
CTGGAGCAGT TGTTAATGCA GCAGAAGCAG AAACAGCAGC GGGGACAAGG TGCCATGAAC 
CCTCCACACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Smg7 ( XM_017319981.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017175470.1 Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OMu77091
Clone ID Related Accession (Same CDS sequence)	NM_001359257.1 , XM_017319981.1
Accession Version	XM_017319981.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2412bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(House mouse)
Product	protein SMG7 isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTTTC TCGGCATCCT GTGCAAATGT CCTCTCCAGA ATGATTCTCA GGAGTCCAAC 
AATGCCTACC CCCTTCCTGC AGTCAAGGTC TCCATGGACT GGCTAAGACT CCGACCTAGA 
GTCTTTCAAG AAGCAGTGGT GGATGAAAGA CAGTACATTT GGCCCTGGCT AATTTCTCTT 
CTAAATAGTT TCCATCCCCG TGAAGATGAT CTCTCAAATA CTAATGCCAC ACCACTTCCA 
GAGGAGTTTG AATTACAAGG ATTCTTGGCT TTAAGACCTT CTTTCAGGAA CTTGGATTTT 
TCCAAAGGCC ATCAGGGTAT TACAGGAGAC AAAGAGGGTC AACAACGACG AATACGACAG 
CAGCGTTTGA TCTCCATAGG GAAATGGATT GCTGATAACC AGCCACGGCT GATTCAGTGT 
GAAAATGAGG TAGGGAAATT GTTATTTATC ACAGAAATCC CAGAATTAAT ACTAGAAGAC 
CCCAGTGAAG CCAAAGAGAA CCTCATCCTA CAAGAAACAT CTGTGGTAGA GTCACTGGCT 
ACGGATGGGA GCCCAGGACT GAAATCAGTG CTGTCTACAG GCCGAAATCC AAGCAACAGC 
TGTGACTCAG GAGAGAAACC AGTGGTCACC TTTAAAGAGA ACATTAAGCC ACGAGAAGTG 
AACCAAGGAA GAAGCTTTCC TCCCAAAGAG GTAAAATCCC AGACAGAACT AAGAAAGACT 
CCAGTGTCTG AAGCCAGGAA AACGCCTGTC ACTCAAACTC CAAGTCAAAC GAGTAATTCT 
CAGTTCATCC CCATCCATCA CCCTGGAGCC TTCCCTCCTC TTCCCAGCCG ACCAGGATTC 
CCGCCCCCAA CATATGTTAT CCCCCCTCCT GTGGCATTTT CTATGGGCTC AGGTTACACC 
TTCCCAGCTG GTGTTTCTGT CCCAGGAACC TTTCTTCAGT CTACAGCTCA CTCTCCAGCA 
GGAAACCAGG TGCAAGCTGG GAAACAGTCC CACATTCCTT ACAGCCAGCA ACGGCCCTCT 
GGACCAGGGC CAATGAACCA GGGACCTCAA CAGTCACAGC CACCTTCCCA GCCACCCCTT 
ACATCTTTAC CAGCTCAGCC AACAGCACAG TCTACAAGCC AATTGCAGGT TCAAGCTCTA 
GCTCAGCAAC AGCAATCCCC TACAAAAGTC ATACCAGCTT TGGGGAAAAG CCCGCCTCAC 
CACTCTGGAT TCCAGCAGTA TCAACAGGCA GATGCCTCCA AACAGCTGTG GAATCCCCCT 
CAGGTTCAAA GCCCACTAGG GAAAATTATG CCTGTGAAAC AGTCCTACTA CCTTCAGACC 
CAAGACCCTA TAAAACTGTT TGAGCCGTCA TTGCAACCTC CTGTAATACA GCAGCAGCCT 
CTAGAGAAAA AAATGAAGCC TTTCCCCATG GAGCCATATA ACCATAATCC CTCAGAAGTC 
AAGGTCCCAG AGTTCTACTG GGATTCTTCC TACAGCATGG CCGATAACAG AGCAGTAATG 
GCTCAGCAAC CAAATATGGA CCGCAGGAGC AAACGGTCAC CTGGAGTCTT CCGTCCAGAA 
CAGGATCCTG TGCCCAGGAT GCCATTTGAG GACCCCAAGA GCTCCCCTCT GCTTCCTCCG 
GACCTGTTAA AGAGTCTGGC TGCCTTGGAG GAAGAGGAAG AGCTGATCTT TTCTAACCCT 
CCTGATCTTT ACCCAGCTCT GCTGGGTCCT CTCGCCTCTC TTCCTGGACG AAGCCTCTTT 
AAATCCTTAT TGGAGAAGCC TTCTGAGCTC ATGTCACATT CATCTTCTTT CCTGTCCCTC 
ACGGGGTTCT CTGTCAATCA GGAAAGATAT CCAAACAGCA GTATGTTCAA TGAAGTGTAT 
GGAAAAAACC TGACAACCAG CTCCAAGGCA GAACTGAACC CCTCAGTCGC CTCCCAGGAA 
ACATCACTGT ACTCCCTTTT TGAAGGGACC CCATGGTCTC CATCACTTCC TGCCAGTTCA 
GATCATTCGA CACCAGCCAG CCAGTCTCCT CATTCCTCTA ACCCAAGCAG CCTGCCCAGT 
TCTCCTCCAA CGCATAACCA TAATTCTGCT CCATTCTCTA ATTTTGGACC CATTGGGACT 
CCAGATAACA GGGATAGGCG GCCTGCAGAT AGGTGGAAAA CTGATAAACC AGCCATGGGT 
GGTTTTGGTG TTGATTATCT CTCAGCAACG TCATCATCTG AGAGCAGTTG GCATCAGGCT 
AGCACTCCGA GTGGCACCTG GACAGGCCAC GGCCCCTCCA TGGAGGATTC CTCTGCTGTC 
CTCATGGAAA GCCTAAAGTC TATCTGGTCC AGTTCCATGA TGCATCCTGG GCCCTCCGCT 
CTGGAGCAGT TGTTAATGCA GCAGAAGCAG AAACAGCAGC GGGGACAAGG TGCCATGAAC 
CCTCCACACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017175470.1
CDS	1255..3666
Translation	MSFLGILCKCPLQNDSQESNNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPREDDLSNTNATPLPEEFELQGFLALRPSFRNLDFSKGHQGITGDKEGQQRRIRQQRLISIGKWIADNQPRLIQCENEVGKLLFITEIPELILEDPSEAKENLILQETSVVESLATDGSPGLKSVLSTGRNPSNSCDSGEKPVVTFKENIKPREVNQGRSFPPKEVKSQTELRKTPVSEARKTPVTQTPSQTSNSQFIPIHHPGAFPPLPSRPGFPPPTYVIPPPVAFSMGSGYTFPAGVSVPGTFLQSTAHSPAGNQVQAGKQSHIPYSQQRPSGPGPMNQGPQQSQPPSQPPLTSLPAQPTAQSTSQLQVQALAQQQQSPTKVIPALGKSPPHHSGFQQYQQADASKQLWNPPQVQSPLGKIMPVKQSYYLQTQDPIKLFEPSLQPPVIQQQPLEKKMKPFPMEPYNHNPSEVKVPEFYWDSSYSMADNRAVMAQQPNMDRRSKRSPGVFRPEQDPVPRMPFEDPKSSPLLPPDLLKSLAALEEEEELIFSNPPDLYPALLGPLASLPGRSLFKSLLEKPSELMSHSSSFLSLTGFSVNQERYPNSSMFNEVYGKNLTTSSKAELNPSVASQETSLYSLFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSAPFSNFGPIGTPDNRDRRPADRWKTDKPAMGGFGVDYLSATSSSESSWHQASTPSGTWTGHGPSMEDSSAVLMESLKSIWSSSMMHPGPSALEQLLMQQKQKQQRGQGAMNPPH

Target ORF information:

RefSeq Version	XM_017319981.1
Organism	Mus musculus(House mouse)
Definition	Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319981.1

ORF Insert Sequence:

ATGTCTTTTC TCGGCATCCT GTGCAAATGT CCTCTCCAGA ATGATTCTCA GGAGTCCAAC 
AATGCCTACC CCCTTCCTGC AGTCAAGGTC TCCATGGACT GGCTAAGACT CCGACCTAGA 
GTCTTTCAAG AAGCAGTGGT GGATGAAAGA CAGTACATTT GGCCCTGGCT AATTTCTCTT 
CTAAATAGTT TCCATCCCCG TGAAGATGAT CTCTCAAATA CTAATGCCAC ACCACTTCCA 
GAGGAGTTTG AATTACAAGG ATTCTTGGCT TTAAGACCTT CTTTCAGGAA CTTGGATTTT 
TCCAAAGGCC ATCAGGGTAT TACAGGAGAC AAAGAGGGTC AACAACGACG AATACGACAG 
CAGCGTTTGA TCTCCATAGG GAAATGGATT GCTGATAACC AGCCACGGCT GATTCAGTGT 
GAAAATGAGG TAGGGAAATT GTTATTTATC ACAGAAATCC CAGAATTAAT ACTAGAAGAC 
CCCAGTGAAG CCAAAGAGAA CCTCATCCTA CAAGAAACAT CTGTGGTAGA GTCACTGGCT 
ACGGATGGGA GCCCAGGACT GAAATCAGTG CTGTCTACAG GCCGAAATCC AAGCAACAGC 
TGTGACTCAG GAGAGAAACC AGTGGTCACC TTTAAAGAGA ACATTAAGCC ACGAGAAGTG 
AACCAAGGAA GAAGCTTTCC TCCCAAAGAG GTAAAATCCC AGACAGAACT AAGAAAGACT 
CCAGTGTCTG AAGCCAGGAA AACGCCTGTC ACTCAAACTC CAAGTCAAAC GAGTAATTCT 
CAGTTCATCC CCATCCATCA CCCTGGAGCC TTCCCTCCTC TTCCCAGCCG ACCAGGATTC 
CCGCCCCCAA CATATGTTAT CCCCCCTCCT GTGGCATTTT CTATGGGCTC AGGTTACACC 
TTCCCAGCTG GTGTTTCTGT CCCAGGAACC TTTCTTCAGT CTACAGCTCA CTCTCCAGCA 
GGAAACCAGG TGCAAGCTGG GAAACAGTCC CACATTCCTT ACAGCCAGCA ACGGCCCTCT 
GGACCAGGGC CAATGAACCA GGGACCTCAA CAGTCACAGC CACCTTCCCA GCCACCCCTT 
ACATCTTTAC CAGCTCAGCC AACAGCACAG TCTACAAGCC AATTGCAGGT TCAAGCTCTA 
GCTCAGCAAC AGCAATCCCC TACAAAAGTC ATACCAGCTT TGGGGAAAAG CCCGCCTCAC 
CACTCTGGAT TCCAGCAGTA TCAACAGGCA GATGCCTCCA AACAGCTGTG GAATCCCCCT 
CAGGTTCAAA GCCCACTAGG GAAAATTATG CCTGTGAAAC AGTCCTACTA CCTTCAGACC 
CAAGACCCTA TAAAACTGTT TGAGCCGTCA TTGCAACCTC CTGTAATACA GCAGCAGCCT 
CTAGAGAAAA AAATGAAGCC TTTCCCCATG GAGCCATATA ACCATAATCC CTCAGAAGTC 
AAGGTCCCAG AGTTCTACTG GGATTCTTCC TACAGCATGG CCGATAACAG AGCAGTAATG 
GCTCAGCAAC CAAATATGGA CCGCAGGAGC AAACGGTCAC CTGGAGTCTT CCGTCCAGAA 
CAGGATCCTG TGCCCAGGAT GCCATTTGAG GACCCCAAGA GCTCCCCTCT GCTTCCTCCG 
GACCTGTTAA AGAGTCTGGC TGCCTTGGAG GAAGAGGAAG AGCTGATCTT TTCTAACCCT 
CCTGATCTTT ACCCAGCTCT GCTGGGTCCT CTCGCCTCTC TTCCTGGACG AAGCCTCTTT 
AAATCCTTAT TGGAGAAGCC TTCTGAGCTC ATGTCACATT CATCTTCTTT CCTGTCCCTC 
ACGGGGTTCT CTGTCAATCA GGAAAGATAT CCAAACAGCA GTATGTTCAA TGAAGTGTAT 
GGAAAAAACC TGACAACCAG CTCCAAGGCA GAACTGAACC CCTCAGTCGC CTCCCAGGAA 
ACATCACTGT ACTCCCTTTT TGAAGGGACC CCATGGTCTC CATCACTTCC TGCCAGTTCA 
GATCATTCGA CACCAGCCAG CCAGTCTCCT CATTCCTCTA ACCCAAGCAG CCTGCCCAGT 
TCTCCTCCAA CGCATAACCA TAATTCTGCT CCATTCTCTA ATTTTGGACC CATTGGGACT 
CCAGATAACA GGGATAGGCG GCCTGCAGAT AGGTGGAAAA CTGATAAACC AGCCATGGGT 
GGTTTTGGTG TTGATTATCT CTCAGCAACG TCATCATCTG AGAGCAGTTG GCATCAGGCT 
AGCACTCCGA GTGGCACCTG GACAGGCCAC GGCCCCTCCA TGGAGGATTC CTCTGCTGTC 
CTCATGGAAA GCCTAAAGTC TATCTGGTCC AGTTCCATGA TGCATCCTGG GCCCTCCGCT 
CTGGAGCAGT TGTTAATGCA GCAGAAGCAG AAACAGCAGC GGGGACAAGG TGCCATGAAC 
CCTCCACACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Smg7 ( XM_030253296.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030109156.1 Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OMu85124
Clone ID Related Accession (Same CDS sequence)	XM_030253296.1 , XM_030253298.1
Accession Version	XM_030253296.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2550bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein SMG7 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTTTC TCGGCATCCT GTGCAAATGT CCTCTCCAGA ATGATTCTCA GGAGTCCAAC 
AATGCCTACC CCCTTCCTGC AGTCAAGGTC TCCATGGACT GGCTAAGACT CCGACCTAGA 
GTCTTTCAAG AAGCAGTGGT GGATGAAAGA CAGTACATTT GGCCCTGGCT AATTTCTCTT 
CTAAATAGTT TCCATCCCCG TGAAGATGAT CTCTCAAATA CTAATGCCAC ACCACTTCCA 
GAGGAGTTTG AATTACAAGG ATTCTTGGCT TTAAGACCTT CTTTCAGGAA CTTGGATTTT 
TCCAAAGGCC ATCAGGGTAT TACAGGAGAC AAAGAGGGTC AACAACGACG AATACGACAG 
CAGCGTTTGA TCTCCATAGG GAAATGGATT GCTGATAACC AGCCACGGCT GATTCAGTGT 
GAAAATGAGG TAGGGAAATT GTTATTTATC ACAGAAATCC CAGAATTAAT ACTAGAAGAC 
CCCAGTGAAG CCAAAGAGAA CCTCATCCTA CAAGAAACAT CTGTGGTAGA GTCACTGGCT 
ACGGATGGGA GCCCAGGACT GAAATCAGTG CTGTCTACAG GCCGAAATCC AAGCAACAGC 
TGTGACTCAG GAGAGAAACC AGTGGTCACC TTTAAAGAGA ACATTAAGCC ACGAGAAGTG 
AACCAAGGAA GAAGCTTTCC TCCCAAAGAG GTGAGAAGGG ACTGTAGCAA AGGCGTAACT 
GTAACTCAGG AGGACGGACA GAAGGACAGC AGCAAGAGGA GAGCTGAGAC CAAGAGATGC 
ACCTTAGGAA AGTTGCAGGA AACAGGAAAG CAGAGTGTGG CAGTGCAGGT AAAATCCCAG 
ACAGAACTAA GAAAGACTCC AGTGTCTGAA GCCAGGAAAA CGCCTGTCAC TCAAACTCCA 
AGTCAAACGA GTAATTCTCA GTTCATCCCC ATCCATCACC CTGGAGCCTT CCCTCCTCTT 
CCCAGCCGAC CAGGATTCCC GCCCCCAACA TATGTTATCC CCCCTCCTGT GGCATTTTCT 
ATGGGCTCAG GTTACACCTT CCCAGCTGGT GTTTCTGTCC CAGGAACCTT TCTTCAGTCT 
ACAGCTCACT CTCCAGCAGG AAACCAGGTG CAAGCTGGGA AACAGTCCCA CATTCCTTAC 
AGCCAGCAAC GGCCCTCTGG ACCAGGGCCA ATGAACCAGG GACCTCAACA GTCACAGCCA 
CCTTCCCAGC CACCCCTTAC ATCTTTACCA GCTCAGCCAA CAGCACAGTC TACAAGCCAA 
TTGCAGGTTC AAGCTCTAGC TCAGCAACAG CAATCCCCTA CAAAAGTCAT ACCAGCTTTG 
GGGAAAAGCC CGCCTCACCA CTCTGGATTC CAGCAGTATC AACAGGCAGA TGCCTCCAAA 
CAGCTGTGGA ATCCCCCTCA GGTTCAAAGC CCACTAGGGA AAATTATGCC TGTGAAACAG 
TCCTACTACC TTCAGACCCA AGACCCTATA AAACTGTTTG AGCCGTCATT GCAACCTCCT 
GTAATACAGC AGCAGCCTCT AGAGAAAAAA ATGAAGCCTT TCCCCATGGA GCCATATAAC 
CATAATCCCT CAGAAGTCAA GGTCCCAGAG TTCTACTGGG ATTCTTCCTA CAGCATGGCC 
GATAACAGAG CAGTAATGGC TCAGCAACCA AATATGGACC GCAGGAGCAA ACGGTCACCT 
GGAGTCTTCC GTCCAGAACA GGATCCTGTG CCCAGGATGC CATTTGAGGA CCCCAAGAGC 
TCCCCTCTGC TTCCTCCGGA CCTGTTAAAG AGTCTGGCTG CCTTGGAGGA AGAGGAAGAG 
CTGATCTTTT CTAACCCTCC TGATCTTTAC CCAGCTCTGC TGGGTCCTCT CGCCTCTCTT 
CCTGGACGAA GCCTCTTTAA ATCCTTATTG GAGAAGCCTT CTGAGCTCAT GTCACATTCA 
TCTTCTTTCC TGTCCCTCAC GGGGTTCTCT GTCAATCAGG AAAGATATCC AAACAGCAGT 
ATGTTCAATG AAGTGTATGG AAAAAACCTG ACAACCAGCT CCAAGGCAGA ACTGAACCCC 
TCAGTCGCCT CCCAGGAAAC ATCACTGTAC TCCCTTTTTG AAGGGACCCC ATGGTCTCCA 
TCACTTCCTG CCAGTTCAGA TCATTCGACA CCAGCCAGCC AGTCTCCTCA TTCCTCTAAC 
CCAAGCAGCC TGCCCAGTTC TCCTCCAACG CATAACCATA ATTCTGCTCC ATTCTCTAAT 
TTTGGACCCA TTGGGACTCC AGATAACAGG GATAGGCGGC CTGCAGATAG GTGGAAAACT 
GATAAACCAG CCATGGGTGG TTTTGGTGTT GATTATCTCT CAGCAACGTC ATCATCTGAG 
AGCAGTTGGC ATCAGGCTAG CACTCCGAGT GGCACCTGGA CAGGCCACGG CCCCTCCATG 
GAGGATTCCT CTGCTGTCCT CATGGAAAGC CTAAAGTCTA TCTGGTCCAG TTCCATGATG 
CATCCTGGGC CCTCCGCTCT GGAGCAGTTG TTAATGCAGC AGAAGCAGAA ACAGCAGCGG 
GGACAAGGTG CCATGAACCC TCCACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030109156.1
CDS	1255..3804
Translation	MSFLGILCKCPLQNDSQESNNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPREDDLSNTNATPLPEEFELQGFLALRPSFRNLDFSKGHQGITGDKEGQQRRIRQQRLISIGKWIADNQPRLIQCENEVGKLLFITEIPELILEDPSEAKENLILQETSVVESLATDGSPGLKSVLSTGRNPSNSCDSGEKPVVTFKENIKPREVNQGRSFPPKEVRRDCSKGVTVTQEDGQKDSSKRRAETKRCTLGKLQETGKQSVAVQVKSQTELRKTPVSEARKTPVTQTPSQTSNSQFIPIHHPGAFPPLPSRPGFPPPTYVIPPPVAFSMGSGYTFPAGVSVPGTFLQSTAHSPAGNQVQAGKQSHIPYSQQRPSGPGPMNQGPQQSQPPSQPPLTSLPAQPTAQSTSQLQVQALAQQQQSPTKVIPALGKSPPHHSGFQQYQQADASKQLWNPPQVQSPLGKIMPVKQSYYLQTQDPIKLFEPSLQPPVIQQQPLEKKMKPFPMEPYNHNPSEVKVPEFYWDSSYSMADNRAVMAQQPNMDRRSKRSPGVFRPEQDPVPRMPFEDPKSSPLLPPDLLKSLAALEEEEELIFSNPPDLYPALLGPLASLPGRSLFKSLLEKPSELMSHSSSFLSLTGFSVNQERYPNSSMFNEVYGKNLTTSSKAELNPSVASQETSLYSLFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSAPFSNFGPIGTPDNRDRRPADRWKTDKPAMGGFGVDYLSATSSSESSWHQASTPSGTWTGHGPSMEDSSAVLMESLKSIWSSSMMHPGPSALEQLLMQQKQKQQRGQGAMNPPH

Target ORF information:

RefSeq Version	XM_030253296.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030253296.1

ORF Insert Sequence:

ATGTCTTTTC TCGGCATCCT GTGCAAATGT CCTCTCCAGA ATGATTCTCA GGAGTCCAAC 
AATGCCTACC CCCTTCCTGC AGTCAAGGTC TCCATGGACT GGCTAAGACT CCGACCTAGA 
GTCTTTCAAG AAGCAGTGGT GGATGAAAGA CAGTACATTT GGCCCTGGCT AATTTCTCTT 
CTAAATAGTT TCCATCCCCG TGAAGATGAT CTCTCAAATA CTAATGCCAC ACCACTTCCA 
GAGGAGTTTG AATTACAAGG ATTCTTGGCT TTAAGACCTT CTTTCAGGAA CTTGGATTTT 
TCCAAAGGCC ATCAGGGTAT TACAGGAGAC AAAGAGGGTC AACAACGACG AATACGACAG 
CAGCGTTTGA TCTCCATAGG GAAATGGATT GCTGATAACC AGCCACGGCT GATTCAGTGT 
GAAAATGAGG TAGGGAAATT GTTATTTATC ACAGAAATCC CAGAATTAAT ACTAGAAGAC 
CCCAGTGAAG CCAAAGAGAA CCTCATCCTA CAAGAAACAT CTGTGGTAGA GTCACTGGCT 
ACGGATGGGA GCCCAGGACT GAAATCAGTG CTGTCTACAG GCCGAAATCC AAGCAACAGC 
TGTGACTCAG GAGAGAAACC AGTGGTCACC TTTAAAGAGA ACATTAAGCC ACGAGAAGTG 
AACCAAGGAA GAAGCTTTCC TCCCAAAGAG GTGAGAAGGG ACTGTAGCAA AGGCGTAACT 
GTAACTCAGG AGGACGGACA GAAGGACAGC AGCAAGAGGA GAGCTGAGAC CAAGAGATGC 
ACCTTAGGAA AGTTGCAGGA AACAGGAAAG CAGAGTGTGG CAGTGCAGGT AAAATCCCAG 
ACAGAACTAA GAAAGACTCC AGTGTCTGAA GCCAGGAAAA CGCCTGTCAC TCAAACTCCA 
AGTCAAACGA GTAATTCTCA GTTCATCCCC ATCCATCACC CTGGAGCCTT CCCTCCTCTT 
CCCAGCCGAC CAGGATTCCC GCCCCCAACA TATGTTATCC CCCCTCCTGT GGCATTTTCT 
ATGGGCTCAG GTTACACCTT CCCAGCTGGT GTTTCTGTCC CAGGAACCTT TCTTCAGTCT 
ACAGCTCACT CTCCAGCAGG AAACCAGGTG CAAGCTGGGA AACAGTCCCA CATTCCTTAC 
AGCCAGCAAC GGCCCTCTGG ACCAGGGCCA ATGAACCAGG GACCTCAACA GTCACAGCCA 
CCTTCCCAGC CACCCCTTAC ATCTTTACCA GCTCAGCCAA CAGCACAGTC TACAAGCCAA 
TTGCAGGTTC AAGCTCTAGC TCAGCAACAG CAATCCCCTA CAAAAGTCAT ACCAGCTTTG 
GGGAAAAGCC CGCCTCACCA CTCTGGATTC CAGCAGTATC AACAGGCAGA TGCCTCCAAA 
CAGCTGTGGA ATCCCCCTCA GGTTCAAAGC CCACTAGGGA AAATTATGCC TGTGAAACAG 
TCCTACTACC TTCAGACCCA AGACCCTATA AAACTGTTTG AGCCGTCATT GCAACCTCCT 
GTAATACAGC AGCAGCCTCT AGAGAAAAAA ATGAAGCCTT TCCCCATGGA GCCATATAAC 
CATAATCCCT CAGAAGTCAA GGTCCCAGAG TTCTACTGGG ATTCTTCCTA CAGCATGGCC 
GATAACAGAG CAGTAATGGC TCAGCAACCA AATATGGACC GCAGGAGCAA ACGGTCACCT 
GGAGTCTTCC GTCCAGAACA GGATCCTGTG CCCAGGATGC CATTTGAGGA CCCCAAGAGC 
TCCCCTCTGC TTCCTCCGGA CCTGTTAAAG AGTCTGGCTG CCTTGGAGGA AGAGGAAGAG 
CTGATCTTTT CTAACCCTCC TGATCTTTAC CCAGCTCTGC TGGGTCCTCT CGCCTCTCTT 
CCTGGACGAA GCCTCTTTAA ATCCTTATTG GAGAAGCCTT CTGAGCTCAT GTCACATTCA 
TCTTCTTTCC TGTCCCTCAC GGGGTTCTCT GTCAATCAGG AAAGATATCC AAACAGCAGT 
ATGTTCAATG AAGTGTATGG AAAAAACCTG ACAACCAGCT CCAAGGCAGA ACTGAACCCC 
TCAGTCGCCT CCCAGGAAAC ATCACTGTAC TCCCTTTTTG AAGGGACCCC ATGGTCTCCA 
TCACTTCCTG CCAGTTCAGA TCATTCGACA CCAGCCAGCC AGTCTCCTCA TTCCTCTAAC 
CCAAGCAGCC TGCCCAGTTC TCCTCCAACG CATAACCATA ATTCTGCTCC ATTCTCTAAT 
TTTGGACCCA TTGGGACTCC AGATAACAGG GATAGGCGGC CTGCAGATAG GTGGAAAACT 
GATAAACCAG CCATGGGTGG TTTTGGTGTT GATTATCTCT CAGCAACGTC ATCATCTGAG 
AGCAGTTGGC ATCAGGCTAG CACTCCGAGT GGCACCTGGA CAGGCCACGG CCCCTCCATG 
GAGGATTCCT CTGCTGTCCT CATGGAAAGC CTAAAGTCTA TCTGGTCCAG TTCCATGATG 
CATCCTGGGC CCTCCGCTCT GGAGCAGTTG TTAATGCAGC AGAAGCAGAA ACAGCAGCGG 
GGACAAGGTG CCATGAACCC TCCACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Smg7 ( XM_030253298.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030109158.1 Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OMu85124
Clone ID Related Accession (Same CDS sequence)	XM_030253296.1 , XM_030253298.1
Accession Version	XM_030253298.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2550bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein SMG7 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTTTC TCGGCATCCT GTGCAAATGT CCTCTCCAGA ATGATTCTCA GGAGTCCAAC 
AATGCCTACC CCCTTCCTGC AGTCAAGGTC TCCATGGACT GGCTAAGACT CCGACCTAGA 
GTCTTTCAAG AAGCAGTGGT GGATGAAAGA CAGTACATTT GGCCCTGGCT AATTTCTCTT 
CTAAATAGTT TCCATCCCCG TGAAGATGAT CTCTCAAATA CTAATGCCAC ACCACTTCCA 
GAGGAGTTTG AATTACAAGG ATTCTTGGCT TTAAGACCTT CTTTCAGGAA CTTGGATTTT 
TCCAAAGGCC ATCAGGGTAT TACAGGAGAC AAAGAGGGTC AACAACGACG AATACGACAG 
CAGCGTTTGA TCTCCATAGG GAAATGGATT GCTGATAACC AGCCACGGCT GATTCAGTGT 
GAAAATGAGG TAGGGAAATT GTTATTTATC ACAGAAATCC CAGAATTAAT ACTAGAAGAC 
CCCAGTGAAG CCAAAGAGAA CCTCATCCTA CAAGAAACAT CTGTGGTAGA GTCACTGGCT 
ACGGATGGGA GCCCAGGACT GAAATCAGTG CTGTCTACAG GCCGAAATCC AAGCAACAGC 
TGTGACTCAG GAGAGAAACC AGTGGTCACC TTTAAAGAGA ACATTAAGCC ACGAGAAGTG 
AACCAAGGAA GAAGCTTTCC TCCCAAAGAG GTGAGAAGGG ACTGTAGCAA AGGCGTAACT 
GTAACTCAGG AGGACGGACA GAAGGACAGC AGCAAGAGGA GAGCTGAGAC CAAGAGATGC 
ACCTTAGGAA AGTTGCAGGA AACAGGAAAG CAGAGTGTGG CAGTGCAGGT AAAATCCCAG 
ACAGAACTAA GAAAGACTCC AGTGTCTGAA GCCAGGAAAA CGCCTGTCAC TCAAACTCCA 
AGTCAAACGA GTAATTCTCA GTTCATCCCC ATCCATCACC CTGGAGCCTT CCCTCCTCTT 
CCCAGCCGAC CAGGATTCCC GCCCCCAACA TATGTTATCC CCCCTCCTGT GGCATTTTCT 
ATGGGCTCAG GTTACACCTT CCCAGCTGGT GTTTCTGTCC CAGGAACCTT TCTTCAGTCT 
ACAGCTCACT CTCCAGCAGG AAACCAGGTG CAAGCTGGGA AACAGTCCCA CATTCCTTAC 
AGCCAGCAAC GGCCCTCTGG ACCAGGGCCA ATGAACCAGG GACCTCAACA GTCACAGCCA 
CCTTCCCAGC CACCCCTTAC ATCTTTACCA GCTCAGCCAA CAGCACAGTC TACAAGCCAA 
TTGCAGGTTC AAGCTCTAGC TCAGCAACAG CAATCCCCTA CAAAAGTCAT ACCAGCTTTG 
GGGAAAAGCC CGCCTCACCA CTCTGGATTC CAGCAGTATC AACAGGCAGA TGCCTCCAAA 
CAGCTGTGGA ATCCCCCTCA GGTTCAAAGC CCACTAGGGA AAATTATGCC TGTGAAACAG 
TCCTACTACC TTCAGACCCA AGACCCTATA AAACTGTTTG AGCCGTCATT GCAACCTCCT 
GTAATACAGC AGCAGCCTCT AGAGAAAAAA ATGAAGCCTT TCCCCATGGA GCCATATAAC 
CATAATCCCT CAGAAGTCAA GGTCCCAGAG TTCTACTGGG ATTCTTCCTA CAGCATGGCC 
GATAACAGAG CAGTAATGGC TCAGCAACCA AATATGGACC GCAGGAGCAA ACGGTCACCT 
GGAGTCTTCC GTCCAGAACA GGATCCTGTG CCCAGGATGC CATTTGAGGA CCCCAAGAGC 
TCCCCTCTGC TTCCTCCGGA CCTGTTAAAG AGTCTGGCTG CCTTGGAGGA AGAGGAAGAG 
CTGATCTTTT CTAACCCTCC TGATCTTTAC CCAGCTCTGC TGGGTCCTCT CGCCTCTCTT 
CCTGGACGAA GCCTCTTTAA ATCCTTATTG GAGAAGCCTT CTGAGCTCAT GTCACATTCA 
TCTTCTTTCC TGTCCCTCAC GGGGTTCTCT GTCAATCAGG AAAGATATCC AAACAGCAGT 
ATGTTCAATG AAGTGTATGG AAAAAACCTG ACAACCAGCT CCAAGGCAGA ACTGAACCCC 
TCAGTCGCCT CCCAGGAAAC ATCACTGTAC TCCCTTTTTG AAGGGACCCC ATGGTCTCCA 
TCACTTCCTG CCAGTTCAGA TCATTCGACA CCAGCCAGCC AGTCTCCTCA TTCCTCTAAC 
CCAAGCAGCC TGCCCAGTTC TCCTCCAACG CATAACCATA ATTCTGCTCC ATTCTCTAAT 
TTTGGACCCA TTGGGACTCC AGATAACAGG GATAGGCGGC CTGCAGATAG GTGGAAAACT 
GATAAACCAG CCATGGGTGG TTTTGGTGTT GATTATCTCT CAGCAACGTC ATCATCTGAG 
AGCAGTTGGC ATCAGGCTAG CACTCCGAGT GGCACCTGGA CAGGCCACGG CCCCTCCATG 
GAGGATTCCT CTGCTGTCCT CATGGAAAGC CTAAAGTCTA TCTGGTCCAG TTCCATGATG 
CATCCTGGGC CCTCCGCTCT GGAGCAGTTG TTAATGCAGC AGAAGCAGAA ACAGCAGCGG 
GGACAAGGTG CCATGAACCC TCCACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030109158.1
CDS	242..2791
Translation	MSFLGILCKCPLQNDSQESNNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPREDDLSNTNATPLPEEFELQGFLALRPSFRNLDFSKGHQGITGDKEGQQRRIRQQRLISIGKWIADNQPRLIQCENEVGKLLFITEIPELILEDPSEAKENLILQETSVVESLATDGSPGLKSVLSTGRNPSNSCDSGEKPVVTFKENIKPREVNQGRSFPPKEVRRDCSKGVTVTQEDGQKDSSKRRAETKRCTLGKLQETGKQSVAVQVKSQTELRKTPVSEARKTPVTQTPSQTSNSQFIPIHHPGAFPPLPSRPGFPPPTYVIPPPVAFSMGSGYTFPAGVSVPGTFLQSTAHSPAGNQVQAGKQSHIPYSQQRPSGPGPMNQGPQQSQPPSQPPLTSLPAQPTAQSTSQLQVQALAQQQQSPTKVIPALGKSPPHHSGFQQYQQADASKQLWNPPQVQSPLGKIMPVKQSYYLQTQDPIKLFEPSLQPPVIQQQPLEKKMKPFPMEPYNHNPSEVKVPEFYWDSSYSMADNRAVMAQQPNMDRRSKRSPGVFRPEQDPVPRMPFEDPKSSPLLPPDLLKSLAALEEEEELIFSNPPDLYPALLGPLASLPGRSLFKSLLEKPSELMSHSSSFLSLTGFSVNQERYPNSSMFNEVYGKNLTTSSKAELNPSVASQETSLYSLFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSAPFSNFGPIGTPDNRDRRPADRWKTDKPAMGGFGVDYLSATSSSESSWHQASTPSGTWTGHGPSMEDSSAVLMESLKSIWSSSMMHPGPSALEQLLMQQKQKQQRGQGAMNPPH

Target ORF information:

RefSeq Version	XM_030253298.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030253298.1

ORF Insert Sequence:

ATGTCTTTTC TCGGCATCCT GTGCAAATGT CCTCTCCAGA ATGATTCTCA GGAGTCCAAC 
AATGCCTACC CCCTTCCTGC AGTCAAGGTC TCCATGGACT GGCTAAGACT CCGACCTAGA 
GTCTTTCAAG AAGCAGTGGT GGATGAAAGA CAGTACATTT GGCCCTGGCT AATTTCTCTT 
CTAAATAGTT TCCATCCCCG TGAAGATGAT CTCTCAAATA CTAATGCCAC ACCACTTCCA 
GAGGAGTTTG AATTACAAGG ATTCTTGGCT TTAAGACCTT CTTTCAGGAA CTTGGATTTT 
TCCAAAGGCC ATCAGGGTAT TACAGGAGAC AAAGAGGGTC AACAACGACG AATACGACAG 
CAGCGTTTGA TCTCCATAGG GAAATGGATT GCTGATAACC AGCCACGGCT GATTCAGTGT 
GAAAATGAGG TAGGGAAATT GTTATTTATC ACAGAAATCC CAGAATTAAT ACTAGAAGAC 
CCCAGTGAAG CCAAAGAGAA CCTCATCCTA CAAGAAACAT CTGTGGTAGA GTCACTGGCT 
ACGGATGGGA GCCCAGGACT GAAATCAGTG CTGTCTACAG GCCGAAATCC AAGCAACAGC 
TGTGACTCAG GAGAGAAACC AGTGGTCACC TTTAAAGAGA ACATTAAGCC ACGAGAAGTG 
AACCAAGGAA GAAGCTTTCC TCCCAAAGAG GTGAGAAGGG ACTGTAGCAA AGGCGTAACT 
GTAACTCAGG AGGACGGACA GAAGGACAGC AGCAAGAGGA GAGCTGAGAC CAAGAGATGC 
ACCTTAGGAA AGTTGCAGGA AACAGGAAAG CAGAGTGTGG CAGTGCAGGT AAAATCCCAG 
ACAGAACTAA GAAAGACTCC AGTGTCTGAA GCCAGGAAAA CGCCTGTCAC TCAAACTCCA 
AGTCAAACGA GTAATTCTCA GTTCATCCCC ATCCATCACC CTGGAGCCTT CCCTCCTCTT 
CCCAGCCGAC CAGGATTCCC GCCCCCAACA TATGTTATCC CCCCTCCTGT GGCATTTTCT 
ATGGGCTCAG GTTACACCTT CCCAGCTGGT GTTTCTGTCC CAGGAACCTT TCTTCAGTCT 
ACAGCTCACT CTCCAGCAGG AAACCAGGTG CAAGCTGGGA AACAGTCCCA CATTCCTTAC 
AGCCAGCAAC GGCCCTCTGG ACCAGGGCCA ATGAACCAGG GACCTCAACA GTCACAGCCA 
CCTTCCCAGC CACCCCTTAC ATCTTTACCA GCTCAGCCAA CAGCACAGTC TACAAGCCAA 
TTGCAGGTTC AAGCTCTAGC TCAGCAACAG CAATCCCCTA CAAAAGTCAT ACCAGCTTTG 
GGGAAAAGCC CGCCTCACCA CTCTGGATTC CAGCAGTATC AACAGGCAGA TGCCTCCAAA 
CAGCTGTGGA ATCCCCCTCA GGTTCAAAGC CCACTAGGGA AAATTATGCC TGTGAAACAG 
TCCTACTACC TTCAGACCCA AGACCCTATA AAACTGTTTG AGCCGTCATT GCAACCTCCT 
GTAATACAGC AGCAGCCTCT AGAGAAAAAA ATGAAGCCTT TCCCCATGGA GCCATATAAC 
CATAATCCCT CAGAAGTCAA GGTCCCAGAG TTCTACTGGG ATTCTTCCTA CAGCATGGCC 
GATAACAGAG CAGTAATGGC TCAGCAACCA AATATGGACC GCAGGAGCAA ACGGTCACCT 
GGAGTCTTCC GTCCAGAACA GGATCCTGTG CCCAGGATGC CATTTGAGGA CCCCAAGAGC 
TCCCCTCTGC TTCCTCCGGA CCTGTTAAAG AGTCTGGCTG CCTTGGAGGA AGAGGAAGAG 
CTGATCTTTT CTAACCCTCC TGATCTTTAC CCAGCTCTGC TGGGTCCTCT CGCCTCTCTT 
CCTGGACGAA GCCTCTTTAA ATCCTTATTG GAGAAGCCTT CTGAGCTCAT GTCACATTCA 
TCTTCTTTCC TGTCCCTCAC GGGGTTCTCT GTCAATCAGG AAAGATATCC AAACAGCAGT 
ATGTTCAATG AAGTGTATGG AAAAAACCTG ACAACCAGCT CCAAGGCAGA ACTGAACCCC 
TCAGTCGCCT CCCAGGAAAC ATCACTGTAC TCCCTTTTTG AAGGGACCCC ATGGTCTCCA 
TCACTTCCTG CCAGTTCAGA TCATTCGACA CCAGCCAGCC AGTCTCCTCA TTCCTCTAAC 
CCAAGCAGCC TGCCCAGTTC TCCTCCAACG CATAACCATA ATTCTGCTCC ATTCTCTAAT 
TTTGGACCCA TTGGGACTCC AGATAACAGG GATAGGCGGC CTGCAGATAG GTGGAAAACT 
GATAAACCAG CCATGGGTGG TTTTGGTGTT GATTATCTCT CAGCAACGTC ATCATCTGAG 
AGCAGTTGGC ATCAGGCTAG CACTCCGAGT GGCACCTGGA CAGGCCACGG CCCCTCCATG 
GAGGATTCCT CTGCTGTCCT CATGGAAAGC CTAAAGTCTA TCTGGTCCAG TTCCATGATG 
CATCCTGGGC CCTCCGCTCT GGAGCAGTTG TTAATGCAGC AGAAGCAGAA ACAGCAGCGG 
GGACAAGGTG CCATGAACCC TCCACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Smg7 ( NM_001160256.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001153728.1 Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant 2, mRNA.

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CloneID	OMu08004
Clone ID Related Accession (Same CDS sequence)	NM_001160256.1
Accession Version	NM_001160256.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3417bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Mus musculus(house mouse)
Product	protein SMG7 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC086651.1, BC082789.1 and AK129101.1. Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: BC086651.1, SRR1660815.17053.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCCTGC AGAGCGCGCA GTATCTCCGG CAGGCAGAAG TCCTGAAGGC TGAAATGACA 
GATTCTAAGC TGGGTCCGGC TGAGGTCTGG ACATCCAGGC AAGCTCTGCA GGACTTGTAC 
CAGAAAATGC TAGTTACTGA TCTGGAATAC GCTTTAGACA AGAAAGTAGA ACAGGATCTC 
TGGAATCATG CCTTTAAGAA TCAGATCACA ACACTGCAAG GCCAAGCCAA GAATCGAGCA 
AACCCAAATC GAAGTGAAGT CCAGGCAAAC CTTTCTCTGT TCCTAGAGGC AGCTAGCGGC 
TTCTATACTC AGTTATTACA GGAACTGTGT ACGGTGTTTA ATGTAGATTT GCCATGCCGT 
GTGAAGTCTT CCCAGTTGGG AATTATTAGC AATAAACAGA CGCACAGCAG CACCATAGTG 
AAGCCACAGT CTAGCTCCTG CTCCTACATT TGCCAACACT GCCTCGTCCA CCTTGGAGAC 
ATTGCTCGAT ACAGAAATCA GACTAGCCAG GCAGAGTCCT ACTATAGGCA TGCAGCTCAG 
CTTGTCCCCT CCAATGGTCA GCCTTACAAT CAGTTGGCTA TCTTAGCTTC TTCCAAAGGA 
GACCACCTGA CCACAATTTT CTACTACTGC AGAAGCATTG CTGTGAAATT CCCTTTCCCA 
GCTGCCTCCA CTAATCTACA AAAAGCACTT TCTAAAGCAC TGGAAAGCCG GGATGAGTTG 
AAAACCAAGT GGGGTGTTTC TGACTTCATC AAGGCCTTTA TTAAATTCCA CGGTCATGTA 
TACCTGAGTA AGAGTTTGGA AAAGCTGAGC CCTCTTCGAG AGAAGTTGGA AGAACAGTTT 
AAGAGACTGC TGTTCCAAAA GGCTTTCAAC TCTCAACAGT TAGTTCATGT CACTGTCATT 
AACCTGTTTC AACTTCATCA TCTTCGTGAC TTTAGCAATG AAACAGAGCA ACACAGTTAC 
AGCCAAGATG AACAGCTGTG TTGGACACAG TTGCTGGCCC TCTTTATGTC TTTTCTCGGC 
ATCCTGTGCA AATGTCCTCT CCAGAATGAT TCTCAGGAGT CCAACAATGC CTACCCCCTT 
CCTGCAGTCA AGGTCTCCAT GGACTGGCTA AGACTCCGAC CTAGAGTCTT TCAAGAAGCA 
GTGGTGGATG AAAGACAGTA CATTTGGCCC TGGCTAATTT CTCTTCTAAA TAGTTTCCAT 
CCCCGTGAAG ATGATCTCTC AAATACTAAT GCCACACCAC TTCCAGAGGA GTTTGAATTA 
CAAGGATTCT TGGCTTTAAG ACCTTCTTTC AGGAACTTGG ATTTTTCCAA AGGCCATCAG 
GGTATTACAG GAGACAAAGA GGGTCAACAA CGACGAATAC GACAGCAGCG TTTGATCTCC 
ATAGGGAAAT GGATTGCTGA TAACCAGCCA CGGCTGATTC AGTGTGAAAA TGAGGTAGGG 
AAATTGTTAT TTATCACAGA AATCCCAGAA TTAATACTAG AAGACCCCAG TGAAGCCAAA 
GAGAACCTCA TCCTACAAGA AACATCTGTG GTAGAGTCAC TGGCTACGGA TGGGAGCCCA 
GGACTGAAAT CAGTGCTGTC TACAGGCCGA AATCCAAGCA ACAGCTGTGA CTCAGGAGAG 
AAACCAGTGG TCACCTTTAA AGAGAACATT AAGCCACGAG AAGTGAACCA AGGAAGAAGC 
TTTCCTCCCA AAGAGGTAAA ATCCCAGACA GAACTAAGAA AGACTCCAGT GTCTGAAGCC 
AGGAAAACGC CTGTCACTCA AACTCCAAGT CAAACGAGTA ATTCTCAGTT CATCCCCATC 
CATCACCCTG GAGCCTTCCC TCCTCTTCCC AGCCGACCAG GATTCCCGCC CCCAACATAT 
GTTATCCCCC CTCCTGTGGC ATTTTCTATG GGCTCAGGTT ACACCTTCCC AGCTGGTGTT 
TCTGTCCCAG GAACCTTTCT TCAGTCTACA GCTCACTCTC CAGCAGGAAA CCAGGTGCAA 
GCTGGGAAAC AGTCCCACAT TCCTTACAGC CAGCAACGGC CCTCTGGACC AGGGCCAATG 
AACCAGGGAC CTCAACAGTC ACAGCCACCT TCCCAGCCAC CCCTTACATC TTTACCAGCT 
CAGCCAACAG CACAGTCTAC AAGCCAATTG CAGGTTCAAG CTCTAGCTCA GCAACAGCAA 
TCCCCTACAA AAGTCATACC AGCTTTGGGG AAAAGCCCGC CTCACCACTC TGGATTCCAG 
CAGTATCAAC AGGCAGATGC CTCCAAACAG CTGTGGAATC CCCCTCAGGT TCAAAGCCCA 
CTAGGGAAAA TTATGCCTGT GAAACAGTCC TACTACCTTC AGACCCAAGA CCCTATAAAA 
CTGTTTGAGC CGTCATTGCA ACCTCCTGTA ATACAGCAGC AGCCTCTAGA GAAAAAAATG 
AAGCCTTTCC CCATGGAGCC ATATAACCAT AATCCCTCAG AAGTCAAGGT CCCAGAGTTC 
TACTGGGATT CTTCCTACAG CATGGCCGAT AACAGAGCAG TAATGGCTCA GCAACCAAAT 
ATGGACCGCA GGAGCAAACG GTCACCTGGA GTCTTCCGTC CAGAACAGGA TCCTGTGCCC 
AGGATGCCAT TTGAGGACCC CAAGAGCTCC CCTCTGCTTC CTCCGGACCT GTTAAAGAGT 
CTGGCTGCCT TGGAGGAAGA GGAAGAGCTG ATCTTTTCTA ACCCTCCTGA TCTTTACCCA 
GCTCTGCTGG GTCCTCTCGC CTCTCTTCCT GGACGAAGCC TCTTTAAATC CTTATTGGAG 
AAGCCTTCTG AGCTCATGTC ACATTCATCT TCTTTCCTGT CCCTCACGGG GTTCTCTGTC 
AATCAGGAAA GATATCCAAA CAGCAGTATG TTCAATGAAG TGTATGGAAA AAACCTGACA 
ACCAGCTCCA AGGCAGAACT GAACCCCTCA GTCGCCTCCC AGGAAACATC ACTGTACTCC 
CTTTTTGAAG GGACCCCATG GTCTCCATCA CTTCCTGCCA GTTCAGATCA TTCGACACCA 
GCCAGCCAGT CTCCTCATTC CTCTAACCCA AGCAGCCTGC CCAGTTCTCC TCCAACGCAT 
AACCATAATT CTGCTCCATT CTCTAATTTT GGACCCATTG GGACTCCAGA TAACAGGGAT 
AGGCGGCCTG CAGATAGGTG GAAAACTGAT AAACCAGCCA TGGGTGGTTT TGGTGTTGAT 
TATCTCTCAG CAACGTCATC ATCTGAGAGC AGTTGGCATC AGGCTAGCAC TCCGAGTGGC 
ACCTGGACAG GCCACGGCCC CTCCATGGAG GATTCCTCTG CTGTCCTCAT GGAAAGCCTA 
AAGTCTATCT GGTCCAGTTC CATGATGCAT CCTGGGCCCT CCGCTCTGGA GCAGTTGTTA 
ATGCAGCAGA AGCAGAAACA GCAGCGGGGA CAAGGTGCCA TGAACCCTCC ACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001153728.1
CDS	110..3526
Translation	MSLQSAQYLRQAEVLKAEMTDSKLGPAEVWTSRQALQDLYQKMLVTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRANPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQTHSSTIVKPQSSSCSYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNLQKALSKALESRDELKTKWGVSDFIKAFIKFHGHVYLSKSLEKLSPLREKLEEQFKRLLFQKAFNSQQLVHVTVINLFQLHHLRDFSNETEQHSYSQDEQLCWTQLLALFMSFLGILCKCPLQNDSQESNNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPREDDLSNTNATPLPEEFELQGFLALRPSFRNLDFSKGHQGITGDKEGQQRRIRQQRLISIGKWIADNQPRLIQCENEVGKLLFITEIPELILEDPSEAKENLILQETSVVESLATDGSPGLKSVLSTGRNPSNSCDSGEKPVVTFKENIKPREVNQGRSFPPKEVKSQTELRKTPVSEARKTPVTQTPSQTSNSQFIPIHHPGAFPPLPSRPGFPPPTYVIPPPVAFSMGSGYTFPAGVSVPGTFLQSTAHSPAGNQVQAGKQSHIPYSQQRPSGPGPMNQGPQQSQPPSQPPLTSLPAQPTAQSTSQLQVQALAQQQQSPTKVIPALGKSPPHHSGFQQYQQADASKQLWNPPQVQSPLGKIMPVKQSYYLQTQDPIKLFEPSLQPPVIQQQPLEKKMKPFPMEPYNHNPSEVKVPEFYWDSSYSMADNRAVMAQQPNMDRRSKRSPGVFRPEQDPVPRMPFEDPKSSPLLPPDLLKSLAALEEEEELIFSNPPDLYPALLGPLASLPGRSLFKSLLEKPSELMSHSSSFLSLTGFSVNQERYPNSSMFNEVYGKNLTTSSKAELNPSVASQETSLYSLFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSAPFSNFGPIGTPDNRDRRPADRWKTDKPAMGGFGVDYLSATSSSESSWHQASTPSGTWTGHGPSMEDSSAVLMESLKSIWSSSMMHPGPSALEQLLMQQKQKQQRGQGAMNPPH

Target ORF information:

RefSeq Version	NM_001160256.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001160256.1

ORF Insert Sequence:

ATGAGCCTGC AGAGCGCGCA GTATCTCCGG CAGGCAGAAG TCCTGAAGGC TGAAATGACA 
GATTCTAAGC TGGGTCCGGC TGAGGTCTGG ACATCCAGGC AAGCTCTGCA GGACTTGTAC 
CAGAAAATGC TAGTTACTGA TCTGGAATAC GCTTTAGACA AGAAAGTAGA ACAGGATCTC 
TGGAATCATG CCTTTAAGAA TCAGATCACA ACACTGCAAG GCCAAGCCAA GAATCGAGCA 
AACCCAAATC GAAGTGAAGT CCAGGCAAAC CTTTCTCTGT TCCTAGAGGC AGCTAGCGGC 
TTCTATACTC AGTTATTACA GGAACTGTGT ACGGTGTTTA ATGTAGATTT GCCATGCCGT 
GTGAAGTCTT CCCAGTTGGG AATTATTAGC AATAAACAGA CGCACAGCAG CACCATAGTG 
AAGCCACAGT CTAGCTCCTG CTCCTACATT TGCCAACACT GCCTCGTCCA CCTTGGAGAC 
ATTGCTCGAT ACAGAAATCA GACTAGCCAG GCAGAGTCCT ACTATAGGCA TGCAGCTCAG 
CTTGTCCCCT CCAATGGTCA GCCTTACAAT CAGTTGGCTA TCTTAGCTTC TTCCAAAGGA 
GACCACCTGA CCACAATTTT CTACTACTGC AGAAGCATTG CTGTGAAATT CCCTTTCCCA 
GCTGCCTCCA CTAATCTACA AAAAGCACTT TCTAAAGCAC TGGAAAGCCG GGATGAGTTG 
AAAACCAAGT GGGGTGTTTC TGACTTCATC AAGGCCTTTA TTAAATTCCA CGGTCATGTA 
TACCTGAGTA AGAGTTTGGA AAAGCTGAGC CCTCTTCGAG AGAAGTTGGA AGAACAGTTT 
AAGAGACTGC TGTTCCAAAA GGCTTTCAAC TCTCAACAGT TAGTTCATGT CACTGTCATT 
AACCTGTTTC AACTTCATCA TCTTCGTGAC TTTAGCAATG AAACAGAGCA ACACAGTTAC 
AGCCAAGATG AACAGCTGTG TTGGACACAG TTGCTGGCCC TCTTTATGTC TTTTCTCGGC 
ATCCTGTGCA AATGTCCTCT CCAGAATGAT TCTCAGGAGT CCAACAATGC CTACCCCCTT 
CCTGCAGTCA AGGTCTCCAT GGACTGGCTA AGACTCCGAC CTAGAGTCTT TCAAGAAGCA 
GTGGTGGATG AAAGACAGTA CATTTGGCCC TGGCTAATTT CTCTTCTAAA TAGTTTCCAT 
CCCCGTGAAG ATGATCTCTC AAATACTAAT GCCACACCAC TTCCAGAGGA GTTTGAATTA 
CAAGGATTCT TGGCTTTAAG ACCTTCTTTC AGGAACTTGG ATTTTTCCAA AGGCCATCAG 
GGTATTACAG GAGACAAAGA GGGTCAACAA CGACGAATAC GACAGCAGCG TTTGATCTCC 
ATAGGGAAAT GGATTGCTGA TAACCAGCCA CGGCTGATTC AGTGTGAAAA TGAGGTAGGG 
AAATTGTTAT TTATCACAGA AATCCCAGAA TTAATACTAG AAGACCCCAG TGAAGCCAAA 
GAGAACCTCA TCCTACAAGA AACATCTGTG GTAGAGTCAC TGGCTACGGA TGGGAGCCCA 
GGACTGAAAT CAGTGCTGTC TACAGGCCGA AATCCAAGCA ACAGCTGTGA CTCAGGAGAG 
AAACCAGTGG TCACCTTTAA AGAGAACATT AAGCCACGAG AAGTGAACCA AGGAAGAAGC 
TTTCCTCCCA AAGAGGTAAA ATCCCAGACA GAACTAAGAA AGACTCCAGT GTCTGAAGCC 
AGGAAAACGC CTGTCACTCA AACTCCAAGT CAAACGAGTA ATTCTCAGTT CATCCCCATC 
CATCACCCTG GAGCCTTCCC TCCTCTTCCC AGCCGACCAG GATTCCCGCC CCCAACATAT 
GTTATCCCCC CTCCTGTGGC ATTTTCTATG GGCTCAGGTT ACACCTTCCC AGCTGGTGTT 
TCTGTCCCAG GAACCTTTCT TCAGTCTACA GCTCACTCTC CAGCAGGAAA CCAGGTGCAA 
GCTGGGAAAC AGTCCCACAT TCCTTACAGC CAGCAACGGC CCTCTGGACC AGGGCCAATG 
AACCAGGGAC CTCAACAGTC ACAGCCACCT TCCCAGCCAC CCCTTACATC TTTACCAGCT 
CAGCCAACAG CACAGTCTAC AAGCCAATTG CAGGTTCAAG CTCTAGCTCA GCAACAGCAA 
TCCCCTACAA AAGTCATACC AGCTTTGGGG AAAAGCCCGC CTCACCACTC TGGATTCCAG 
CAGTATCAAC AGGCAGATGC CTCCAAACAG CTGTGGAATC CCCCTCAGGT TCAAAGCCCA 
CTAGGGAAAA TTATGCCTGT GAAACAGTCC TACTACCTTC AGACCCAAGA CCCTATAAAA 
CTGTTTGAGC CGTCATTGCA ACCTCCTGTA ATACAGCAGC AGCCTCTAGA GAAAAAAATG 
AAGCCTTTCC CCATGGAGCC ATATAACCAT AATCCCTCAG AAGTCAAGGT CCCAGAGTTC 
TACTGGGATT CTTCCTACAG CATGGCCGAT AACAGAGCAG TAATGGCTCA GCAACCAAAT 
ATGGACCGCA GGAGCAAACG GTCACCTGGA GTCTTCCGTC CAGAACAGGA TCCTGTGCCC 
AGGATGCCAT TTGAGGACCC CAAGAGCTCC CCTCTGCTTC CTCCGGACCT GTTAAAGAGT 
CTGGCTGCCT TGGAGGAAGA GGAAGAGCTG ATCTTTTCTA ACCCTCCTGA TCTTTACCCA 
GCTCTGCTGG GTCCTCTCGC CTCTCTTCCT GGACGAAGCC TCTTTAAATC CTTATTGGAG 
AAGCCTTCTG AGCTCATGTC ACATTCATCT TCTTTCCTGT CCCTCACGGG GTTCTCTGTC 
AATCAGGAAA GATATCCAAA CAGCAGTATG TTCAATGAAG TGTATGGAAA AAACCTGACA 
ACCAGCTCCA AGGCAGAACT GAACCCCTCA GTCGCCTCCC AGGAAACATC ACTGTACTCC 
CTTTTTGAAG GGACCCCATG GTCTCCATCA CTTCCTGCCA GTTCAGATCA TTCGACACCA 
GCCAGCCAGT CTCCTCATTC CTCTAACCCA AGCAGCCTGC CCAGTTCTCC TCCAACGCAT 
AACCATAATT CTGCTCCATT CTCTAATTTT GGACCCATTG GGACTCCAGA TAACAGGGAT 
AGGCGGCCTG CAGATAGGTG GAAAACTGAT AAACCAGCCA TGGGTGGTTT TGGTGTTGAT 
TATCTCTCAG CAACGTCATC ATCTGAGAGC AGTTGGCATC AGGCTAGCAC TCCGAGTGGC 
ACCTGGACAG GCCACGGCCC CTCCATGGAG GATTCCTCTG CTGTCCTCAT GGAAAGCCTA 
AAGTCTATCT GGTCCAGTTC CATGATGCAT CCTGGGCCCT CCGCTCTGGA GCAGTTGTTA 
ATGCAGCAGA AGCAGAAACA GCAGCGGGGA CAAGGTGCCA TGAACCCTCC ACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Smg7 ( NM_001005507.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001005507.1 Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu10690
Clone ID Related Accession (Same CDS sequence)	NM_001005507.2
Accession Version	NM_001005507.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3405bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Mus musculus(house mouse)
Product	protein SMG7 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC086651.1, BC082789.1 and AK129101.1. On May 16, 2009 this sequence version replaced NM_001005507.1. Transcript Variant: This variant (3) differs in the 5' UTR and has several differences in the coding sequence compared to variant 1. The resulting isoform (3) has a distinct N-terminus and the protein is shorter compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: BC082789.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCCTGC AGAGCGCGCA GTATCTCCGG CAGGCAGAAG TCCTGAAGGC TGAAATGACA 
GATTCTAAGC TGGGTCCGGC TGAGGTCTGG ACATCCAGGC AAGCTCTGCA GGACTTGTAC 
CAGAAAATGC TAGTTACTGA TCTGGAATAC GCTTTAGACA AGAAAGTAGA ACAGGATCTC 
TGGAATCATG CCTTTAAGAA TCAGATCACA ACACTGCAAG GCCAAGCCAA GAATCGAGCA 
AACCCAAATC GAAGTGAAGT CCAGGCAAAC CTTTCTCTGT TCCTAGAGGC AGCTAGCGGC 
TTCTATACTC AGTTATTACA GGAACTGTGT ACGGTGTTTA ATGTAGATTT GCCATGCCGT 
GTGAAGTCTT CCCAGTTGGG AATTATTAGC AATAAACAGA CGCACAGCAG CACCATAGTG 
AAGCCACAGT CTAGCTCCTG CTCCTACATT TGCCAACACT GCCTCGTCCA CCTTGGAGAC 
ATTGCTCGAT ACAGAAATCA GACTAGCCAG GCAGAGTCCT ACTATAGGCA TGCAGCTCAG 
CTTGTCCCCT CCAATGGTCA GCCTTACAAT CAGTTGGCTA TCTTAGCTTC TTCCAAAGGA 
GACCACCTGA CCACAATTTT CTACTACTGC AGAAGCATTG CTGTGAAATT CCCTTTCCCA 
GCTGCCTCCA CTAATCTACA AAAAGCACTT TCTAAAGCAC TGGAAAGCCG GGATGAGTTG 
AAAACCAAGT GGGGTGTTTC TGACTTCATC AAGGCCTTTA TTAAATTCCA CGGTCATGTA 
TACCTGAGTA AGAGTTTGGA AAAGCTGAGC CCTCTTCGAG AGAAGTTGGA AGAACAGTTT 
AAGAGACTGC TGTTCCAAAA GGCTTTCAAC TCTCAACAGT TAGTTCATGT CACTGTCATT 
AACCTGTTTC AACTTCATCA TCTTCGTGAC TTTAGCAATG AAACAGAGCA ACACAGTTAC 
AGCCAAGATG AACAGCTGTG TTGGACACAG TTGCTGGCCC TCTTTATGTC TTTTCTCGGC 
ATCCTGTGCA AATGTCCTCT CCAGAATGAT TCTCAGGAGT CCAACAATGC CTACCCCCTT 
CCTGCAGTCA AGGTCTCCAT GGACTGGCTA AGACTCCGAC CTAGAGTCTT TCAAGAAGCA 
GTGGTGGATG AAAGACAGTA CATTTGGCCC TGGCTAATTT CTCTTCTAAA TAGTTTCCAT 
CCCCGTGAAG ATGATCTCTC AAATACTAAT GCCACACCAC TTCCAGAGGA GTTTGAATTA 
CAAGGATTCT TGGCTTTAAG ACCTTCTTTC AGGAACTTGG ATTTTTCCAA AGGCCATCAG 
GGTATTACAG GAGACAAAGA GGGTCAACAA CGACGAATAC GACAGCAGCG TTTGATCTCC 
ATAGGGAAAT GGATTGCTGA TAACCAGCCA CGGCTGATTC AGTGTGAAAA TGAGGTAGGG 
AAATTGTTAT TTATCACAGA AATCCCAGAA TTAATACTAG AAGACCCCAG TGAAGCCAAA 
GAGAACCTCA TCCTACAAGA AACATCTGTG GTAGAGTCAC TGGCTACGGA TGGGAGCCCA 
GGACTGAAAT CAGTGCTGTC TACAGGCCGA AATCCAAGCA ACAGCTGTGA CTCAGGAGAG 
AAACCAGTGG TCACCTTTAA AGAGAACATT AAGCCACGAG AAGTGAACCA AGGAAGAAGC 
TTTCCTCCCA AAGAGGTGAG AAGGGACTGT AGCAAAGGCG TAACTGTAAC TCAGGAGGAC 
GGACAGAAGG ACAGCAGCAA GAGGAGAGCT GAGACCAAGA GATGCACCTT AGGAAAGTTG 
CAGGAAACAG GAAAGCAGAG TGTGGCAGTG CAGGTAAAAT CCCAGACAGA ACTAAGAAAG 
ACTCCAGTGT CTGAAGCCAG GAAAACGCCT GTCACTCAAA CTCCAAGTCA AACGAGTAAT 
TCTCAGTTCA TCCCCATCCA TCACCCTGGA GCCTTCCCTC CTCTTCCCAG CCGACCAGGA 
TTCCCGCCCC CAACATATGT TATCCCCCCT CCTGTGGCAT TTTCTATGGG CTCAGGTTAC 
ACCTTCCCAG CTGGTGTTTC TGTCCCAGGA ACCTTTCTTC AGTCTACAGC TCACTCTCCA 
GCAGGAAACC AGGTGCAAGC TGGGAAACAG TCCCACATTC CTTACAGCCA GCAACGGCCC 
TCTGGACCAG GGCCAATGAA CCAGGGACCT CAACAGTCAC AGCCACCTTC CCAGCCACCC 
CTTACATCTT TACCAGCTCA GCCAACAGCA CAGTCTACAA GCCAATTGCA GGTTCAAGCT 
CTAGCTCAGC AACAGCAATC CCCTACAAAA GTCATACCAG CTTTGGGGAA AAGCCCGCCT 
CACCACTCTG GATTCCAGCA GTATCAACAG GCAGATGCCT CCAAACAGCT GTGGAATCCC 
CCTCAGGTTC AAAGCCCACT AGGGAAAATT ATGCCTGTGA AACAGTCCTA CTACCTTCAG 
ACCCAAGACC CTATAAAACT GTTTGAGCCG TCATTGCAAC CTCCTGTAAT ACAGCAGCAG 
CCTCTAGAGA AAAAAATGAA GCCTTTCCCC ATGGAGCCAT ATAACCATAA TCCCTCAGAA 
GTCAAGGTCC CAGAGTTCTA CTGGGATTCT TCCTACAGCA TGGCCGATAA CAGAGCAGTA 
ATGGCTCAGC AACCAAATAT GGACCGCAGG AGCAAACGGT CACCTGGAGT CTTCCGTCCA 
GAACAGGATC CTGTGCCCAG GATGCCATTT GAGAAATCCT TATTGGAGAA GCCTTCTGAG 
CTCATGTCAC ATTCATCTTC TTTCCTGTCC CTCACGGGGT TCTCTGTCAA TCAGGAAAGA 
TATCCAAACA GCAGTATGTT CAATGAAGTG TATGGAAAAA ACCTGACAAC CAGCTCCAAG 
GCAGAACTGA ACCCCTCAGT CGCCTCCCAG GAAACATCAC TGTACTCCCT TTTTGAAGGG 
ACCCCATGGT CTCCATCACT TCCTGCCAGT TCAGATCATT CGACACCAGC CAGCCAGTCT 
CCTCATTCCT CTAACCCAAG CAGCCTGCCC AGTTCTCCTC CAACGCATAA CCATAATTCT 
GCTCCATTCT CTAATTTTGG ACCCATTGGG ACTCCAGATA ACAGGGATAG GCGGCCTGCA 
GATAGGTGGA AAACTGATAA ACCAGCCATG GGTGGTTTTG GTGTTGATTA TCTCTCAGCA 
ACGTCATCAT CTGAGAGCAG TTGGCATCAG GCTAGCACTC CGAGTGGCAC CTGGACAGGC 
CACGGCCCCT CCATGGAGGA TTCCTCTGCT GTCCTCATGG AAAGCCTAAA GTCTATCTGG 
TCCAGTTCCA TGATGCATCC TGGGCCCTCC GCTCTGGAGC AGTTGTTAAT GCAGCAGAAG 
CAGAAACAGC AGCGGGGACA AGGTGCCATG AACCCTCCAC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001005507.1
CDS	110..3514
Translation	MSLQSAQYLRQAEVLKAEMTDSKLGPAEVWTSRQALQDLYQKMLVTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRANPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQTHSSTIVKPQSSSCSYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNLQKALSKALESRDELKTKWGVSDFIKAFIKFHGHVYLSKSLEKLSPLREKLEEQFKRLLFQKAFNSQQLVHVTVINLFQLHHLRDFSNETEQHSYSQDEQLCWTQLLALFMSFLGILCKCPLQNDSQESNNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPREDDLSNTNATPLPEEFELQGFLALRPSFRNLDFSKGHQGITGDKEGQQRRIRQQRLISIGKWIADNQPRLIQCENEVGKLLFITEIPELILEDPSEAKENLILQETSVVESLATDGSPGLKSVLSTGRNPSNSCDSGEKPVVTFKENIKPREVNQGRSFPPKEVRRDCSKGVTVTQEDGQKDSSKRRAETKRCTLGKLQETGKQSVAVQVKSQTELRKTPVSEARKTPVTQTPSQTSNSQFIPIHHPGAFPPLPSRPGFPPPTYVIPPPVAFSMGSGYTFPAGVSVPGTFLQSTAHSPAGNQVQAGKQSHIPYSQQRPSGPGPMNQGPQQSQPPSQPPLTSLPAQPTAQSTSQLQVQALAQQQQSPTKVIPALGKSPPHHSGFQQYQQADASKQLWNPPQVQSPLGKIMPVKQSYYLQTQDPIKLFEPSLQPPVIQQQPLEKKMKPFPMEPYNHNPSEVKVPEFYWDSSYSMADNRAVMAQQPNMDRRSKRSPGVFRPEQDPVPRMPFEKSLLEKPSELMSHSSSFLSLTGFSVNQERYPNSSMFNEVYGKNLTTSSKAELNPSVASQETSLYSLFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSAPFSNFGPIGTPDNRDRRPADRWKTDKPAMGGFGVDYLSATSSSESSWHQASTPSGTWTGHGPSMEDSSAVLMESLKSIWSSSMMHPGPSALEQLLMQQKQKQQRGQGAMNPPH

Target ORF information:

RefSeq Version	NM_001005507.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001005507.2

ORF Insert Sequence:

ATGAGCCTGC AGAGCGCGCA GTATCTCCGG CAGGCAGAAG TCCTGAAGGC TGAAATGACA 
GATTCTAAGC TGGGTCCGGC TGAGGTCTGG ACATCCAGGC AAGCTCTGCA GGACTTGTAC 
CAGAAAATGC TAGTTACTGA TCTGGAATAC GCTTTAGACA AGAAAGTAGA ACAGGATCTC 
TGGAATCATG CCTTTAAGAA TCAGATCACA ACACTGCAAG GCCAAGCCAA GAATCGAGCA 
AACCCAAATC GAAGTGAAGT CCAGGCAAAC CTTTCTCTGT TCCTAGAGGC AGCTAGCGGC 
TTCTATACTC AGTTATTACA GGAACTGTGT ACGGTGTTTA ATGTAGATTT GCCATGCCGT 
GTGAAGTCTT CCCAGTTGGG AATTATTAGC AATAAACAGA CGCACAGCAG CACCATAGTG 
AAGCCACAGT CTAGCTCCTG CTCCTACATT TGCCAACACT GCCTCGTCCA CCTTGGAGAC 
ATTGCTCGAT ACAGAAATCA GACTAGCCAG GCAGAGTCCT ACTATAGGCA TGCAGCTCAG 
CTTGTCCCCT CCAATGGTCA GCCTTACAAT CAGTTGGCTA TCTTAGCTTC TTCCAAAGGA 
GACCACCTGA CCACAATTTT CTACTACTGC AGAAGCATTG CTGTGAAATT CCCTTTCCCA 
GCTGCCTCCA CTAATCTACA AAAAGCACTT TCTAAAGCAC TGGAAAGCCG GGATGAGTTG 
AAAACCAAGT GGGGTGTTTC TGACTTCATC AAGGCCTTTA TTAAATTCCA CGGTCATGTA 
TACCTGAGTA AGAGTTTGGA AAAGCTGAGC CCTCTTCGAG AGAAGTTGGA AGAACAGTTT 
AAGAGACTGC TGTTCCAAAA GGCTTTCAAC TCTCAACAGT TAGTTCATGT CACTGTCATT 
AACCTGTTTC AACTTCATCA TCTTCGTGAC TTTAGCAATG AAACAGAGCA ACACAGTTAC 
AGCCAAGATG AACAGCTGTG TTGGACACAG TTGCTGGCCC TCTTTATGTC TTTTCTCGGC 
ATCCTGTGCA AATGTCCTCT CCAGAATGAT TCTCAGGAGT CCAACAATGC CTACCCCCTT 
CCTGCAGTCA AGGTCTCCAT GGACTGGCTA AGACTCCGAC CTAGAGTCTT TCAAGAAGCA 
GTGGTGGATG AAAGACAGTA CATTTGGCCC TGGCTAATTT CTCTTCTAAA TAGTTTCCAT 
CCCCGTGAAG ATGATCTCTC AAATACTAAT GCCACACCAC TTCCAGAGGA GTTTGAATTA 
CAAGGATTCT TGGCTTTAAG ACCTTCTTTC AGGAACTTGG ATTTTTCCAA AGGCCATCAG 
GGTATTACAG GAGACAAAGA GGGTCAACAA CGACGAATAC GACAGCAGCG TTTGATCTCC 
ATAGGGAAAT GGATTGCTGA TAACCAGCCA CGGCTGATTC AGTGTGAAAA TGAGGTAGGG 
AAATTGTTAT TTATCACAGA AATCCCAGAA TTAATACTAG AAGACCCCAG TGAAGCCAAA 
GAGAACCTCA TCCTACAAGA AACATCTGTG GTAGAGTCAC TGGCTACGGA TGGGAGCCCA 
GGACTGAAAT CAGTGCTGTC TACAGGCCGA AATCCAAGCA ACAGCTGTGA CTCAGGAGAG 
AAACCAGTGG TCACCTTTAA AGAGAACATT AAGCCACGAG AAGTGAACCA AGGAAGAAGC 
TTTCCTCCCA AAGAGGTGAG AAGGGACTGT AGCAAAGGCG TAACTGTAAC TCAGGAGGAC 
GGACAGAAGG ACAGCAGCAA GAGGAGAGCT GAGACCAAGA GATGCACCTT AGGAAAGTTG 
CAGGAAACAG GAAAGCAGAG TGTGGCAGTG CAGGTAAAAT CCCAGACAGA ACTAAGAAAG 
ACTCCAGTGT CTGAAGCCAG GAAAACGCCT GTCACTCAAA CTCCAAGTCA AACGAGTAAT 
TCTCAGTTCA TCCCCATCCA TCACCCTGGA GCCTTCCCTC CTCTTCCCAG CCGACCAGGA 
TTCCCGCCCC CAACATATGT TATCCCCCCT CCTGTGGCAT TTTCTATGGG CTCAGGTTAC 
ACCTTCCCAG CTGGTGTTTC TGTCCCAGGA ACCTTTCTTC AGTCTACAGC TCACTCTCCA 
GCAGGAAACC AGGTGCAAGC TGGGAAACAG TCCCACATTC CTTACAGCCA GCAACGGCCC 
TCTGGACCAG GGCCAATGAA CCAGGGACCT CAACAGTCAC AGCCACCTTC CCAGCCACCC 
CTTACATCTT TACCAGCTCA GCCAACAGCA CAGTCTACAA GCCAATTGCA GGTTCAAGCT 
CTAGCTCAGC AACAGCAATC CCCTACAAAA GTCATACCAG CTTTGGGGAA AAGCCCGCCT 
CACCACTCTG GATTCCAGCA GTATCAACAG GCAGATGCCT CCAAACAGCT GTGGAATCCC 
CCTCAGGTTC AAAGCCCACT AGGGAAAATT ATGCCTGTGA AACAGTCCTA CTACCTTCAG 
ACCCAAGACC CTATAAAACT GTTTGAGCCG TCATTGCAAC CTCCTGTAAT ACAGCAGCAG 
CCTCTAGAGA AAAAAATGAA GCCTTTCCCC ATGGAGCCAT ATAACCATAA TCCCTCAGAA 
GTCAAGGTCC CAGAGTTCTA CTGGGATTCT TCCTACAGCA TGGCCGATAA CAGAGCAGTA 
ATGGCTCAGC AACCAAATAT GGACCGCAGG AGCAAACGGT CACCTGGAGT CTTCCGTCCA 
GAACAGGATC CTGTGCCCAG GATGCCATTT GAGAAATCCT TATTGGAGAA GCCTTCTGAG 
CTCATGTCAC ATTCATCTTC TTTCCTGTCC CTCACGGGGT TCTCTGTCAA TCAGGAAAGA 
TATCCAAACA GCAGTATGTT CAATGAAGTG TATGGAAAAA ACCTGACAAC CAGCTCCAAG 
GCAGAACTGA ACCCCTCAGT CGCCTCCCAG GAAACATCAC TGTACTCCCT TTTTGAAGGG 
ACCCCATGGT CTCCATCACT TCCTGCCAGT TCAGATCATT CGACACCAGC CAGCCAGTCT 
CCTCATTCCT CTAACCCAAG CAGCCTGCCC AGTTCTCCTC CAACGCATAA CCATAATTCT 
GCTCCATTCT CTAATTTTGG ACCCATTGGG ACTCCAGATA ACAGGGATAG GCGGCCTGCA 
GATAGGTGGA AAACTGATAA ACCAGCCATG GGTGGTTTTG GTGTTGATTA TCTCTCAGCA 
ACGTCATCAT CTGAGAGCAG TTGGCATCAG GCTAGCACTC CGAGTGGCAC CTGGACAGGC 
CACGGCCCCT CCATGGAGGA TTCCTCTGCT GTCCTCATGG AAAGCCTAAA GTCTATCTGG 
TCCAGTTCCA TGATGCATCC TGGGCCCTCC GCTCTGGAGC AGTTGTTAAT GCAGCAGAAG 
CAGAAACAGC AGCGGGGACA AGGTGCCATG AACCCTCCAC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Smg7 ( XM_006529450.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529513.1 Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant X1, mRNA.

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CloneID	OMu28138
Clone ID Related Accession (Same CDS sequence)	XM_006529450.4 , XM_006529450.3
Accession Version	XM_006529450.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3579bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein SMG7 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006529450.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGACCG AAAACTTGAA ATCAGAGGAG CATCTGAAAT CAAGTAATAT TAGGCAGGCA 
GAAGTCCTGA AGGCTGAAAT GACAGATTCT AAGCTGGGTC CGGCTGAGGT CTGGACATCC 
AGGCAAGCTC TGCAGGACTT GTACCAGAAA ATGCTAGTTA CTGATCTGGA ATACGCTTTA 
GACAAGAAAG TAGAACAGGA TCTCTGGAAT CATGCCTTTA AGAATCAGAT CACAACACTG 
CAAGGCCAAG CCAAGAATCG AGCAAACCCA AATCGAAGTG AAGTCCAGGC AAACCTTTCT 
CTGTTCCTAG AGGCAGCTAG CGGCTTCTAT ACTCAGTTAT TACAGGAACT GTGTACGGTG 
TTTAATGTAG ATTTGCCATG CCGTGTGAAG TCTTCCCAGT TGGGAATTAT TAGCAATAAA 
CAGACGCACA GCAGCACCAT AGTGAAGCCA CAGTCTAGCT CCTGCTCCTA CATTTGCCAA 
CACTGCCTCG TCCACCTTGG AGACATTGCT CGATACAGAA ATCAGACTAG CCAGGCAGAG 
TCCTACTATA GGCATGCAGC TCAGCTTGTC CCCTCCAATG GTCAGCCTTA CAATCAGTTG 
GCTATCTTAG CTTCTTCCAA AGGAGACCAC CTGACCACAA TTTTCTACTA CTGCAGAAGC 
ATTGCTGTGA AATTCCCTTT CCCAGCTGCC TCCACTAATC TACAAAAAGC ACTTTCTAAA 
GCACTGGAAA GCCGGGATGA GTTGAAAACC AAGTGGGGTG TTTCTGACTT CATCAAGGCC 
TTTATTAAAT TCCACGGTCA TGTATACCTG AGTAAGAGTT TGGAAAAGCT GAGCCCTCTT 
CGAGAGAAGT TGGAAGAACA GTTTAAGAGA CTGCTGTTCC AAAAGGCTTT CAACTCTCAA 
CAGTTAGTTC ATGTCACTGT CATTAACCTG TTTCAACTTC ATCATCTTCG TGACTTTAGC 
AATGAAACAG AGCAACACAG TTACAGCCAA GATGAACAGC TGTGTTGGAC ACAGTTGCTG 
GCCCTCTTTA TGTCTTTTCT CGGCATCCTG TGCAAATGTC CTCTCCAGAA TGATTCTCAG 
GAGTCCAACA ATGCCTACCC CCTTCCTGCA GTCAAGGTCT CCATGGACTG GCTAAGACTC 
CGACCTAGAG TCTTTCAAGA AGCAGTGGTG GATGAAAGAC AGTACATTTG GCCCTGGCTA 
ATTTCTCTTC TAAATAGTTT CCATCCCCGT GAAGATGATC TCTCAAATAC TAATGCCACA 
CCACTTCCAG AGGAGTTTGA ATTACAAGGA TTCTTGGCTT TAAGACCTTC TTTCAGGAAC 
TTGGATTTTT CCAAAGGCCA TCAGGGTATT ACAGGAGACA AAGAGGGTCA ACAACGACGA 
ATACGACAGC AGCGTTTGAT CTCCATAGGG AAATGGATTG CTGATAACCA GCCACGGCTG 
ATTCAGTGTG AAAATGAGGT AGGGAAATTG TTATTTATCA CAGAAATCCC AGAATTAATA 
CTAGAAGACC CCAGTGAAGC CAAAGAGAAC CTCATCCTAC AAGAAACATC TGTGGTAGAG 
TCACTGGCTA CGGATGGGAG CCCAGGACTG AAATCAGTGC TGTCTACAGG CCGAAATCCA 
AGCAACAGCT GTGACTCAGG AGAGAAACCA GTGGTCACCT TTAAAGAGAA CATTAAGCCA 
CGAGAAGTGA ACCAAGGAAG AAGCTTTCCT CCCAAAGAGG TGAGAAGGGA CTGTAGCAAA 
GGCGTAACTG TAACTCAGGA GGACGGACAG AAGGACAGCA GCAAGAGGAG AGCTGAGACC 
AAGAGATGCA CCTTAGGAAA GTTGCAGGAA ACAGGAAAGC AGAGTGTGGC AGTGCAGGTA 
AAATCCCAGA CAGAACTAAG AAAGACTCCA GTGTCTGAAG CCAGGAAAAC GCCTGTCACT 
CAAACTCCAA GTCAAACGAG TAATTCTCAG TTCATCCCCA TCCATCACCC TGGAGCCTTC 
CCTCCTCTTC CCAGCCGACC AGGATTCCCG CCCCCAACAT ATGTTATCCC CCCTCCTGTG 
GCATTTTCTA TGGGCTCAGG TTACACCTTC CCAGCTGGTG TTTCTGTCCC AGGAACCTTT 
CTTCAGTCTA CAGCTCACTC TCCAGCAGGA AACCAGGTGC AAGCTGGGAA ACAGTCCCAC 
ATTCCTTACA GCCAGCAACG GCCCTCTGGA CCAGGGCCAA TGAACCAGGG ACCTCAACAG 
TCACAGCCAC CTTCCCAGCC ACCCCTTACA TCTTTACCAG CTCAGCCAAC AGCACAGTCT 
ACAAGCCAAT TGCAGGTTCA AGCTCTAGCT CAGCAACAGC AATCCCCTAC AAAAGTCATA 
CCAGCTTTGG GGAAAAGCCC GCCTCACCAC TCTGGATTCC AGCAGTATCA ACAGGCAGAT 
GCCTCCAAAC AGCTGTGGAA TCCCCCTCAG GTTCAAAGCC CACTAGGGAA AATTATGCCT 
GTGAAACAGT CCTACTACCT TCAGACCCAA GACCCTATAA AACTGTTTGA GCCGTCATTG 
CAACCTCCTG TAATACAGCA GCAGCCTCTA GAGAAAAAAA TGAAGCCTTT CCCCATGGAG 
CCATATAACC ATAATCCCTC AGAAGTCAAG GTCCCAGAGT TCTACTGGGA TTCTTCCTAC 
AGCATGGCCG ATAACAGAGC AGTAATGGCT CAGCAACCAA ATATGGACCG CAGGAGCAAA 
CGGTCACCTG GAGTCTTCCG TCCAGAACAG GATCCTGTGC CCAGGATGCC ATTTGAGGAC 
CCCAAGAGCT CCCCTCTGCT TCCTCCGGAC CTGTTAAAGA GTCTGGCTGC CTTGGAGGAA 
GAGGAAGAGC TGATCTTTTC TAACCCTCCT GATCTTTACC CAGCTCTGCT GGGTCCTCTC 
GCCTCTCTTC CTGGACGAAG CCTCTTTAAA TCCTTATTGG AGAAGCCTTC TGAGCTCATG 
TCACATTCAT CTTCTTTCCT GTCCCTCACG GGGTTCTCTG TCAATCAGGA AAGATATCCA 
AACAGCAGTA TGTTCAATGA AGTGTATGGA AAAAACCTGA CAACCAGCTC CAAGGCAGAA 
CTGAACCCCT CAGTCGCCTC CCAGGAAACA TCACTGTACT CCCTTTTTGA AGGGACCCCA 
TGGTCTCCAT CACTTCCTGC CAGTTCAGAT CATTCGACAC CAGCCAGCCA GTCTCCTCAT 
TCCTCTAACC CAAGCAGCCT GCCCAGTTCT CCTCCAACGC ATAACCATAA TTCTGCTCCA 
TTCTCTAATT TTGGACCCAT TGGGACTCCA GATAACAGGG ATAGGCGGCC TGCAGATAGG 
TGGAAAACTG ATAAACCAGC CATGGGTGGT TTTGGTGTTG ATTATCTCTC AGCAACGTCA 
TCATCTGAGA GCAGTTGGCA TCAGGCTAGC ACTCCGAGTG GCACCTGGAC AGGCCACGGC 
CCCTCCATGG AGGATTCCTC TGCTGTCCTC ATGGAAAGCC TAAAGTCTAT CTGGTCCAGT 
TCCATGATGC ATCCTGGGCC CTCCGCTCTG GAGCAGTTGT TAATGCAGCA GAAGCAGAAA 
CAGCAGCGGG GACAAGGTGC CATGAACCCT CCACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529513.1
CDS	707..4285
Translation	MRTENLKSEEHLKSSNIRQAEVLKAEMTDSKLGPAEVWTSRQALQDLYQKMLVTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRANPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQTHSSTIVKPQSSSCSYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNLQKALSKALESRDELKTKWGVSDFIKAFIKFHGHVYLSKSLEKLSPLREKLEEQFKRLLFQKAFNSQQLVHVTVINLFQLHHLRDFSNETEQHSYSQDEQLCWTQLLALFMSFLGILCKCPLQNDSQESNNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPREDDLSNTNATPLPEEFELQGFLALRPSFRNLDFSKGHQGITGDKEGQQRRIRQQRLISIGKWIADNQPRLIQCENEVGKLLFITEIPELILEDPSEAKENLILQETSVVESLATDGSPGLKSVLSTGRNPSNSCDSGEKPVVTFKENIKPREVNQGRSFPPKEVRRDCSKGVTVTQEDGQKDSSKRRAETKRCTLGKLQETGKQSVAVQVKSQTELRKTPVSEARKTPVTQTPSQTSNSQFIPIHHPGAFPPLPSRPGFPPPTYVIPPPVAFSMGSGYTFPAGVSVPGTFLQSTAHSPAGNQVQAGKQSHIPYSQQRPSGPGPMNQGPQQSQPPSQPPLTSLPAQPTAQSTSQLQVQALAQQQQSPTKVIPALGKSPPHHSGFQQYQQADASKQLWNPPQVQSPLGKIMPVKQSYYLQTQDPIKLFEPSLQPPVIQQQPLEKKMKPFPMEPYNHNPSEVKVPEFYWDSSYSMADNRAVMAQQPNMDRRSKRSPGVFRPEQDPVPRMPFEDPKSSPLLPPDLLKSLAALEEEEELIFSNPPDLYPALLGPLASLPGRSLFKSLLEKPSELMSHSSSFLSLTGFSVNQERYPNSSMFNEVYGKNLTTSSKAELNPSVASQETSLYSLFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSAPFSNFGPIGTPDNRDRRPADRWKTDKPAMGGFGVDYLSATSSSESSWHQASTPSGTWTGHGPSMEDSSAVLMESLKSIWSSSMMHPGPSALEQLLMQQKQKQQRGQGAMNPPH

Target ORF information:

RefSeq Version	XM_006529450.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006529450.4

ORF Insert Sequence:

ATGAGGACCG AAAACTTGAA ATCAGAGGAG CATCTGAAAT CAAGTAATAT TAGGCAGGCA 
GAAGTCCTGA AGGCTGAAAT GACAGATTCT AAGCTGGGTC CGGCTGAGGT CTGGACATCC 
AGGCAAGCTC TGCAGGACTT GTACCAGAAA ATGCTAGTTA CTGATCTGGA ATACGCTTTA 
GACAAGAAAG TAGAACAGGA TCTCTGGAAT CATGCCTTTA AGAATCAGAT CACAACACTG 
CAAGGCCAAG CCAAGAATCG AGCAAACCCA AATCGAAGTG AAGTCCAGGC AAACCTTTCT 
CTGTTCCTAG AGGCAGCTAG CGGCTTCTAT ACTCAGTTAT TACAGGAACT GTGTACGGTG 
TTTAATGTAG ATTTGCCATG CCGTGTGAAG TCTTCCCAGT TGGGAATTAT TAGCAATAAA 
CAGACGCACA GCAGCACCAT AGTGAAGCCA CAGTCTAGCT CCTGCTCCTA CATTTGCCAA 
CACTGCCTCG TCCACCTTGG AGACATTGCT CGATACAGAA ATCAGACTAG CCAGGCAGAG 
TCCTACTATA GGCATGCAGC TCAGCTTGTC CCCTCCAATG GTCAGCCTTA CAATCAGTTG 
GCTATCTTAG CTTCTTCCAA AGGAGACCAC CTGACCACAA TTTTCTACTA CTGCAGAAGC 
ATTGCTGTGA AATTCCCTTT CCCAGCTGCC TCCACTAATC TACAAAAAGC ACTTTCTAAA 
GCACTGGAAA GCCGGGATGA GTTGAAAACC AAGTGGGGTG TTTCTGACTT CATCAAGGCC 
TTTATTAAAT TCCACGGTCA TGTATACCTG AGTAAGAGTT TGGAAAAGCT GAGCCCTCTT 
CGAGAGAAGT TGGAAGAACA GTTTAAGAGA CTGCTGTTCC AAAAGGCTTT CAACTCTCAA 
CAGTTAGTTC ATGTCACTGT CATTAACCTG TTTCAACTTC ATCATCTTCG TGACTTTAGC 
AATGAAACAG AGCAACACAG TTACAGCCAA GATGAACAGC TGTGTTGGAC ACAGTTGCTG 
GCCCTCTTTA TGTCTTTTCT CGGCATCCTG TGCAAATGTC CTCTCCAGAA TGATTCTCAG 
GAGTCCAACA ATGCCTACCC CCTTCCTGCA GTCAAGGTCT CCATGGACTG GCTAAGACTC 
CGACCTAGAG TCTTTCAAGA AGCAGTGGTG GATGAAAGAC AGTACATTTG GCCCTGGCTA 
ATTTCTCTTC TAAATAGTTT CCATCCCCGT GAAGATGATC TCTCAAATAC TAATGCCACA 
CCACTTCCAG AGGAGTTTGA ATTACAAGGA TTCTTGGCTT TAAGACCTTC TTTCAGGAAC 
TTGGATTTTT CCAAAGGCCA TCAGGGTATT ACAGGAGACA AAGAGGGTCA ACAACGACGA 
ATACGACAGC AGCGTTTGAT CTCCATAGGG AAATGGATTG CTGATAACCA GCCACGGCTG 
ATTCAGTGTG AAAATGAGGT AGGGAAATTG TTATTTATCA CAGAAATCCC AGAATTAATA 
CTAGAAGACC CCAGTGAAGC CAAAGAGAAC CTCATCCTAC AAGAAACATC TGTGGTAGAG 
TCACTGGCTA CGGATGGGAG CCCAGGACTG AAATCAGTGC TGTCTACAGG CCGAAATCCA 
AGCAACAGCT GTGACTCAGG AGAGAAACCA GTGGTCACCT TTAAAGAGAA CATTAAGCCA 
CGAGAAGTGA ACCAAGGAAG AAGCTTTCCT CCCAAAGAGG TGAGAAGGGA CTGTAGCAAA 
GGCGTAACTG TAACTCAGGA GGACGGACAG AAGGACAGCA GCAAGAGGAG AGCTGAGACC 
AAGAGATGCA CCTTAGGAAA GTTGCAGGAA ACAGGAAAGC AGAGTGTGGC AGTGCAGGTA 
AAATCCCAGA CAGAACTAAG AAAGACTCCA GTGTCTGAAG CCAGGAAAAC GCCTGTCACT 
CAAACTCCAA GTCAAACGAG TAATTCTCAG TTCATCCCCA TCCATCACCC TGGAGCCTTC 
CCTCCTCTTC CCAGCCGACC AGGATTCCCG CCCCCAACAT ATGTTATCCC CCCTCCTGTG 
GCATTTTCTA TGGGCTCAGG TTACACCTTC CCAGCTGGTG TTTCTGTCCC AGGAACCTTT 
CTTCAGTCTA CAGCTCACTC TCCAGCAGGA AACCAGGTGC AAGCTGGGAA ACAGTCCCAC 
ATTCCTTACA GCCAGCAACG GCCCTCTGGA CCAGGGCCAA TGAACCAGGG ACCTCAACAG 
TCACAGCCAC CTTCCCAGCC ACCCCTTACA TCTTTACCAG CTCAGCCAAC AGCACAGTCT 
ACAAGCCAAT TGCAGGTTCA AGCTCTAGCT CAGCAACAGC AATCCCCTAC AAAAGTCATA 
CCAGCTTTGG GGAAAAGCCC GCCTCACCAC TCTGGATTCC AGCAGTATCA ACAGGCAGAT 
GCCTCCAAAC AGCTGTGGAA TCCCCCTCAG GTTCAAAGCC CACTAGGGAA AATTATGCCT 
GTGAAACAGT CCTACTACCT TCAGACCCAA GACCCTATAA AACTGTTTGA GCCGTCATTG 
CAACCTCCTG TAATACAGCA GCAGCCTCTA GAGAAAAAAA TGAAGCCTTT CCCCATGGAG 
CCATATAACC ATAATCCCTC AGAAGTCAAG GTCCCAGAGT TCTACTGGGA TTCTTCCTAC 
AGCATGGCCG ATAACAGAGC AGTAATGGCT CAGCAACCAA ATATGGACCG CAGGAGCAAA 
CGGTCACCTG GAGTCTTCCG TCCAGAACAG GATCCTGTGC CCAGGATGCC ATTTGAGGAC 
CCCAAGAGCT CCCCTCTGCT TCCTCCGGAC CTGTTAAAGA GTCTGGCTGC CTTGGAGGAA 
GAGGAAGAGC TGATCTTTTC TAACCCTCCT GATCTTTACC CAGCTCTGCT GGGTCCTCTC 
GCCTCTCTTC CTGGACGAAG CCTCTTTAAA TCCTTATTGG AGAAGCCTTC TGAGCTCATG 
TCACATTCAT CTTCTTTCCT GTCCCTCACG GGGTTCTCTG TCAATCAGGA AAGATATCCA 
AACAGCAGTA TGTTCAATGA AGTGTATGGA AAAAACCTGA CAACCAGCTC CAAGGCAGAA 
CTGAACCCCT CAGTCGCCTC CCAGGAAACA TCACTGTACT CCCTTTTTGA AGGGACCCCA 
TGGTCTCCAT CACTTCCTGC CAGTTCAGAT CATTCGACAC CAGCCAGCCA GTCTCCTCAT 
TCCTCTAACC CAAGCAGCCT GCCCAGTTCT CCTCCAACGC ATAACCATAA TTCTGCTCCA 
TTCTCTAATT TTGGACCCAT TGGGACTCCA GATAACAGGG ATAGGCGGCC TGCAGATAGG 
TGGAAAACTG ATAAACCAGC CATGGGTGGT TTTGGTGTTG ATTATCTCTC AGCAACGTCA 
TCATCTGAGA GCAGTTGGCA TCAGGCTAGC ACTCCGAGTG GCACCTGGAC AGGCCACGGC 
CCCTCCATGG AGGATTCCTC TGCTGTCCTC ATGGAAAGCC TAAAGTCTAT CTGGTCCAGT 
TCCATGATGC ATCCTGGGCC CTCCGCTCTG GAGCAGTTGT TAATGCAGCA GAAGCAGAAA 
CAGCAGCGGG GACAAGGTGC CATGAACCCT CCACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Smg7 ( XM_006529451.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529514.1 Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant X2, mRNA.

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CloneID	OMu28139
Clone ID Related Accession (Same CDS sequence)	XM_006529451.3 , XM_006529451.4
Accession Version	XM_006529451.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3555bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein SMG7 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006529451.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCTGC AGAGCGCGCA GTATCTCCGG CAGGCAGAAG TCCTGAAGGC TGAAATGACA 
GATTCTAAGC TGGGTCCGGC TGAGGTCTGG ACATCCAGGC AAGCTCTGCA GGACTTGTAC 
CAGAAAATGC TAGTTACTGA TCTGGAATAC GCTTTAGACA AGAAAGTAGA ACAGGATCTC 
TGGAATCATG CCTTTAAGAA TCAGATCACA ACACTGCAAG GCCAAGCCAA GAATCGAGCA 
AACCCAAATC GAAGTGAAGT CCAGGCAAAC CTTTCTCTGT TCCTAGAGGC AGCTAGCGGC 
TTCTATACTC AGTTATTACA GGAACTGTGT ACGGTGTTTA ATGTAGATTT GCCATGCCGT 
GTGAAGTCTT CCCAGTTGGG AATTATTAGC AATAAACAGA CGCACAGCAG CACCATAGTG 
AAGCCACAGT CTAGCTCCTG CTCCTACATT TGCCAACACT GCCTCGTCCA CCTTGGAGAC 
ATTGCTCGAT ACAGAAATCA GACTAGCCAG GCAGAGTCCT ACTATAGGCA TGCAGCTCAG 
CTTGTCCCCT CCAATGGTCA GCCTTACAAT CAGTTGGCTA TCTTAGCTTC TTCCAAAGGA 
GACCACCTGA CCACAATTTT CTACTACTGC AGAAGCATTG CTGTGAAATT CCCTTTCCCA 
GCTGCCTCCA CTAATCTACA AAAAGCACTT TCTAAAGCAC TGGAAAGCCG GGATGAGTTG 
AAAACCAAGT GGGGTGTTTC TGACTTCATC AAGGCCTTTA TTAAATTCCA CGGTCATGTA 
TACCTGAGTA AGAGTTTGGA AAAGCTGAGC CCTCTTCGAG AGAAGTTGGA AGAACAGTTT 
AAGAGACTGC TGTTCCAAAA GGCTTTCAAC TCTCAACAGT TAGTTCATGT CACTGTCATT 
AACCTGTTTC AACTTCATCA TCTTCGTGAC TTTAGCAATG AAACAGAGCA ACACAGTTAC 
AGCCAAGATG AACAGCTGTG TTGGACACAG TTGCTGGCCC TCTTTATGTC TTTTCTCGGC 
ATCCTGTGCA AATGTCCTCT CCAGAATGAT TCTCAGGAGT CCAACAATGC CTACCCCCTT 
CCTGCAGTCA AGGTCTCCAT GGACTGGCTA AGACTCCGAC CTAGAGTCTT TCAAGAAGCA 
GTGGTGGATG AAAGACAGTA CATTTGGCCC TGGCTAATTT CTCTTCTAAA TAGTTTCCAT 
CCCCGTGAAG ATGATCTCTC AAATACTAAT GCCACACCAC TTCCAGAGGA GTTTGAATTA 
CAAGGATTCT TGGCTTTAAG ACCTTCTTTC AGGAACTTGG ATTTTTCCAA AGGCCATCAG 
GGTATTACAG GAGACAAAGA GGGTCAACAA CGACGAATAC GACAGCAGCG TTTGATCTCC 
ATAGGGAAAT GGATTGCTGA TAACCAGCCA CGGCTGATTC AGTGTGAAAA TGAGGTAGGG 
AAATTGTTAT TTATCACAGA AATCCCAGAA TTAATACTAG AAGACCCCAG TGAAGCCAAA 
GAGAACCTCA TCCTACAAGA AACATCTGTG GTAGAGTCAC TGGCTACGGA TGGGAGCCCA 
GGACTGAAAT CAGTGCTGTC TACAGGCCGA AATCCAAGCA ACAGCTGTGA CTCAGGAGAG 
AAACCAGTGG TCACCTTTAA AGAGAACATT AAGCCACGAG AAGTGAACCA AGGAAGAAGC 
TTTCCTCCCA AAGAGGTGAG AAGGGACTGT AGCAAAGGCG TAACTGTAAC TCAGGAGGAC 
GGACAGAAGG ACAGCAGCAA GAGGAGAGCT GAGACCAAGA GATGCACCTT AGGAAAGTTG 
CAGGAAACAG GAAAGCAGAG TGTGGCAGTG CAGGTAAAAT CCCAGACAGA ACTAAGAAAG 
ACTCCAGTGT CTGAAGCCAG GAAAACGCCT GTCACTCAAA CTCCAAGTCA AACGAGTAAT 
TCTCAGTTCA TCCCCATCCA TCACCCTGGA GCCTTCCCTC CTCTTCCCAG CCGACCAGGA 
TTCCCGCCCC CAACATATGT TATCCCCCCT CCTGTGGCAT TTTCTATGGG CTCAGGTTAC 
ACCTTCCCAG CTGGTGTTTC TGTCCCAGGA ACCTTTCTTC AGTCTACAGC TCACTCTCCA 
GCAGGAAACC AGGTGCAAGC TGGGAAACAG TCCCACATTC CTTACAGCCA GCAACGGCCC 
TCTGGACCAG GGCCAATGAA CCAGGGACCT CAACAGTCAC AGCCACCTTC CCAGCCACCC 
CTTACATCTT TACCAGCTCA GCCAACAGCA CAGTCTACAA GCCAATTGCA GGTTCAAGCT 
CTAGCTCAGC AACAGCAATC CCCTACAAAA GTCATACCAG CTTTGGGGAA AAGCCCGCCT 
CACCACTCTG GATTCCAGCA GTATCAACAG GCAGATGCCT CCAAACAGCT GTGGAATCCC 
CCTCAGGTTC AAAGCCCACT AGGGAAAATT ATGCCTGTGA AACAGTCCTA CTACCTTCAG 
ACCCAAGACC CTATAAAACT GTTTGAGCCG TCATTGCAAC CTCCTGTAAT ACAGCAGCAG 
CCTCTAGAGA AAAAAATGAA GCCTTTCCCC ATGGAGCCAT ATAACCATAA TCCCTCAGAA 
GTCAAGGTCC CAGAGTTCTA CTGGGATTCT TCCTACAGCA TGGCCGATAA CAGAGCAGTA 
ATGGCTCAGC AACCAAATAT GGACCGCAGG AGCAAACGGT CACCTGGAGT CTTCCGTCCA 
GAACAGGATC CTGTGCCCAG GATGCCATTT GAGGACCCCA AGAGCTCCCC TCTGCTTCCT 
CCGGACCTGT TAAAGAGTCT GGCTGCCTTG GAGGAAGAGG AAGAGCTGAT CTTTTCTAAC 
CCTCCTGATC TTTACCCAGC TCTGCTGGGT CCTCTCGCCT CTCTTCCTGG ACGAAGCCTC 
TTTAAATCCT TATTGGAGAA GCCTTCTGAG CTCATGTCAC ATTCATCTTC TTTCCTGTCC 
CTCACGGGGT TCTCTGTCAA TCAGGAAAGA TATCCAAACA GCAGTATGTT CAATGAAGTG 
TATGGAAAAA ACCTGACAAC CAGCTCCAAG GCAGAACTGA ACCCCTCAGT CGCCTCCCAG 
GAAACATCAC TGTACTCCCT TTTTGAAGGG ACCCCATGGT CTCCATCACT TCCTGCCAGT 
TCAGATCATT CGACACCAGC CAGCCAGTCT CCTCATTCCT CTAACCCAAG CAGCCTGCCC 
AGTTCTCCTC CAACGCATAA CCATAATTCT GCTCCATTCT CTAATTTTGG ACCCATTGGG 
ACTCCAGATA ACAGGGATAG GCGGCCTGCA GATAGGTGGA AAACTGATAA ACCAGCCATG 
GGTGGTTTTG GTGTTGATTA TCTCTCAGCA ACGTCATCAT CTGAGAGCAG TTGGCATCAG 
GCTAGCACTC CGAGTGGCAC CTGGACAGGC CACGGCCCCT CCATGGAGGA TTCCTCTGCT 
GTCCTCATGG AAAGCCTAAA GTCTATCTGG TCCAGTTCCA TGATGCATCC TGGGCCCTCC 
GCTCTGGAGC AGTTGTTAAT GCAGCAGAAG CAGAAACAGC AGCGGGGACA AGGTGCCATG 
AACCCTCCAC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529514.1
CDS	594..4148
Translation	MSLQSAQYLRQAEVLKAEMTDSKLGPAEVWTSRQALQDLYQKMLVTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRANPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQTHSSTIVKPQSSSCSYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNLQKALSKALESRDELKTKWGVSDFIKAFIKFHGHVYLSKSLEKLSPLREKLEEQFKRLLFQKAFNSQQLVHVTVINLFQLHHLRDFSNETEQHSYSQDEQLCWTQLLALFMSFLGILCKCPLQNDSQESNNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPREDDLSNTNATPLPEEFELQGFLALRPSFRNLDFSKGHQGITGDKEGQQRRIRQQRLISIGKWIADNQPRLIQCENEVGKLLFITEIPELILEDPSEAKENLILQETSVVESLATDGSPGLKSVLSTGRNPSNSCDSGEKPVVTFKENIKPREVNQGRSFPPKEVRRDCSKGVTVTQEDGQKDSSKRRAETKRCTLGKLQETGKQSVAVQVKSQTELRKTPVSEARKTPVTQTPSQTSNSQFIPIHHPGAFPPLPSRPGFPPPTYVIPPPVAFSMGSGYTFPAGVSVPGTFLQSTAHSPAGNQVQAGKQSHIPYSQQRPSGPGPMNQGPQQSQPPSQPPLTSLPAQPTAQSTSQLQVQALAQQQQSPTKVIPALGKSPPHHSGFQQYQQADASKQLWNPPQVQSPLGKIMPVKQSYYLQTQDPIKLFEPSLQPPVIQQQPLEKKMKPFPMEPYNHNPSEVKVPEFYWDSSYSMADNRAVMAQQPNMDRRSKRSPGVFRPEQDPVPRMPFEDPKSSPLLPPDLLKSLAALEEEEELIFSNPPDLYPALLGPLASLPGRSLFKSLLEKPSELMSHSSSFLSLTGFSVNQERYPNSSMFNEVYGKNLTTSSKAELNPSVASQETSLYSLFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSAPFSNFGPIGTPDNRDRRPADRWKTDKPAMGGFGVDYLSATSSSESSWHQASTPSGTWTGHGPSMEDSSAVLMESLKSIWSSSMMHPGPSALEQLLMQQKQKQQRGQGAMNPPH

Target ORF information:

RefSeq Version	XM_006529451.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006529451.4

ORF Insert Sequence:

ATGAGCCTGC AGAGCGCGCA GTATCTCCGG CAGGCAGAAG TCCTGAAGGC TGAAATGACA 
GATTCTAAGC TGGGTCCGGC TGAGGTCTGG ACATCCAGGC AAGCTCTGCA GGACTTGTAC 
CAGAAAATGC TAGTTACTGA TCTGGAATAC GCTTTAGACA AGAAAGTAGA ACAGGATCTC 
TGGAATCATG CCTTTAAGAA TCAGATCACA ACACTGCAAG GCCAAGCCAA GAATCGAGCA 
AACCCAAATC GAAGTGAAGT CCAGGCAAAC CTTTCTCTGT TCCTAGAGGC AGCTAGCGGC 
TTCTATACTC AGTTATTACA GGAACTGTGT ACGGTGTTTA ATGTAGATTT GCCATGCCGT 
GTGAAGTCTT CCCAGTTGGG AATTATTAGC AATAAACAGA CGCACAGCAG CACCATAGTG 
AAGCCACAGT CTAGCTCCTG CTCCTACATT TGCCAACACT GCCTCGTCCA CCTTGGAGAC 
ATTGCTCGAT ACAGAAATCA GACTAGCCAG GCAGAGTCCT ACTATAGGCA TGCAGCTCAG 
CTTGTCCCCT CCAATGGTCA GCCTTACAAT CAGTTGGCTA TCTTAGCTTC TTCCAAAGGA 
GACCACCTGA CCACAATTTT CTACTACTGC AGAAGCATTG CTGTGAAATT CCCTTTCCCA 
GCTGCCTCCA CTAATCTACA AAAAGCACTT TCTAAAGCAC TGGAAAGCCG GGATGAGTTG 
AAAACCAAGT GGGGTGTTTC TGACTTCATC AAGGCCTTTA TTAAATTCCA CGGTCATGTA 
TACCTGAGTA AGAGTTTGGA AAAGCTGAGC CCTCTTCGAG AGAAGTTGGA AGAACAGTTT 
AAGAGACTGC TGTTCCAAAA GGCTTTCAAC TCTCAACAGT TAGTTCATGT CACTGTCATT 
AACCTGTTTC AACTTCATCA TCTTCGTGAC TTTAGCAATG AAACAGAGCA ACACAGTTAC 
AGCCAAGATG AACAGCTGTG TTGGACACAG TTGCTGGCCC TCTTTATGTC TTTTCTCGGC 
ATCCTGTGCA AATGTCCTCT CCAGAATGAT TCTCAGGAGT CCAACAATGC CTACCCCCTT 
CCTGCAGTCA AGGTCTCCAT GGACTGGCTA AGACTCCGAC CTAGAGTCTT TCAAGAAGCA 
GTGGTGGATG AAAGACAGTA CATTTGGCCC TGGCTAATTT CTCTTCTAAA TAGTTTCCAT 
CCCCGTGAAG ATGATCTCTC AAATACTAAT GCCACACCAC TTCCAGAGGA GTTTGAATTA 
CAAGGATTCT TGGCTTTAAG ACCTTCTTTC AGGAACTTGG ATTTTTCCAA AGGCCATCAG 
GGTATTACAG GAGACAAAGA GGGTCAACAA CGACGAATAC GACAGCAGCG TTTGATCTCC 
ATAGGGAAAT GGATTGCTGA TAACCAGCCA CGGCTGATTC AGTGTGAAAA TGAGGTAGGG 
AAATTGTTAT TTATCACAGA AATCCCAGAA TTAATACTAG AAGACCCCAG TGAAGCCAAA 
GAGAACCTCA TCCTACAAGA AACATCTGTG GTAGAGTCAC TGGCTACGGA TGGGAGCCCA 
GGACTGAAAT CAGTGCTGTC TACAGGCCGA AATCCAAGCA ACAGCTGTGA CTCAGGAGAG 
AAACCAGTGG TCACCTTTAA AGAGAACATT AAGCCACGAG AAGTGAACCA AGGAAGAAGC 
TTTCCTCCCA AAGAGGTGAG AAGGGACTGT AGCAAAGGCG TAACTGTAAC TCAGGAGGAC 
GGACAGAAGG ACAGCAGCAA GAGGAGAGCT GAGACCAAGA GATGCACCTT AGGAAAGTTG 
CAGGAAACAG GAAAGCAGAG TGTGGCAGTG CAGGTAAAAT CCCAGACAGA ACTAAGAAAG 
ACTCCAGTGT CTGAAGCCAG GAAAACGCCT GTCACTCAAA CTCCAAGTCA AACGAGTAAT 
TCTCAGTTCA TCCCCATCCA TCACCCTGGA GCCTTCCCTC CTCTTCCCAG CCGACCAGGA 
TTCCCGCCCC CAACATATGT TATCCCCCCT CCTGTGGCAT TTTCTATGGG CTCAGGTTAC 
ACCTTCCCAG CTGGTGTTTC TGTCCCAGGA ACCTTTCTTC AGTCTACAGC TCACTCTCCA 
GCAGGAAACC AGGTGCAAGC TGGGAAACAG TCCCACATTC CTTACAGCCA GCAACGGCCC 
TCTGGACCAG GGCCAATGAA CCAGGGACCT CAACAGTCAC AGCCACCTTC CCAGCCACCC 
CTTACATCTT TACCAGCTCA GCCAACAGCA CAGTCTACAA GCCAATTGCA GGTTCAAGCT 
CTAGCTCAGC AACAGCAATC CCCTACAAAA GTCATACCAG CTTTGGGGAA AAGCCCGCCT 
CACCACTCTG GATTCCAGCA GTATCAACAG GCAGATGCCT CCAAACAGCT GTGGAATCCC 
CCTCAGGTTC AAAGCCCACT AGGGAAAATT ATGCCTGTGA AACAGTCCTA CTACCTTCAG 
ACCCAAGACC CTATAAAACT GTTTGAGCCG TCATTGCAAC CTCCTGTAAT ACAGCAGCAG 
CCTCTAGAGA AAAAAATGAA GCCTTTCCCC ATGGAGCCAT ATAACCATAA TCCCTCAGAA 
GTCAAGGTCC CAGAGTTCTA CTGGGATTCT TCCTACAGCA TGGCCGATAA CAGAGCAGTA 
ATGGCTCAGC AACCAAATAT GGACCGCAGG AGCAAACGGT CACCTGGAGT CTTCCGTCCA 
GAACAGGATC CTGTGCCCAG GATGCCATTT GAGGACCCCA AGAGCTCCCC TCTGCTTCCT 
CCGGACCTGT TAAAGAGTCT GGCTGCCTTG GAGGAAGAGG AAGAGCTGAT CTTTTCTAAC 
CCTCCTGATC TTTACCCAGC TCTGCTGGGT CCTCTCGCCT CTCTTCCTGG ACGAAGCCTC 
TTTAAATCCT TATTGGAGAA GCCTTCTGAG CTCATGTCAC ATTCATCTTC TTTCCTGTCC 
CTCACGGGGT TCTCTGTCAA TCAGGAAAGA TATCCAAACA GCAGTATGTT CAATGAAGTG 
TATGGAAAAA ACCTGACAAC CAGCTCCAAG GCAGAACTGA ACCCCTCAGT CGCCTCCCAG 
GAAACATCAC TGTACTCCCT TTTTGAAGGG ACCCCATGGT CTCCATCACT TCCTGCCAGT 
TCAGATCATT CGACACCAGC CAGCCAGTCT CCTCATTCCT CTAACCCAAG CAGCCTGCCC 
AGTTCTCCTC CAACGCATAA CCATAATTCT GCTCCATTCT CTAATTTTGG ACCCATTGGG 
ACTCCAGATA ACAGGGATAG GCGGCCTGCA GATAGGTGGA AAACTGATAA ACCAGCCATG 
GGTGGTTTTG GTGTTGATTA TCTCTCAGCA ACGTCATCAT CTGAGAGCAG TTGGCATCAG 
GCTAGCACTC CGAGTGGCAC CTGGACAGGC CACGGCCCCT CCATGGAGGA TTCCTCTGCT 
GTCCTCATGG AAAGCCTAAA GTCTATCTGG TCCAGTTCCA TGATGCATCC TGGGCCCTCC 
GCTCTGGAGC AGTTGTTAAT GCAGCAGAAG CAGAAACAGC AGCGGGGACA AGGTGCCATG 
AACCCTCCAC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Smg7 ( XM_030253290.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030109150.1 Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant X3, mRNA.

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CloneID	OMu28140
Clone ID Related Accession (Same CDS sequence)	XM_017319978.1 , XM_030253290.1
Accession Version	XM_030253290.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3429bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein SMG7 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGACCG AAAACTTGAA ATCAGAGGAG CATCTGAAAT CAAGTAATAT TAGGCAGGCA 
GAAGTCCTGA AGGCTGAAAT GACAGATTCT AAGCTGGGTC CGGCTGAGGT CTGGACATCC 
AGGCAAGCTC TGCAGGACTT GTACCAGAAA ATGCTAGTTA CTGATCTGGA ATACGCTTTA 
GACAAGAAAG TAGAACAGGA TCTCTGGAAT CATGCCTTTA AGAATCAGAT CACAACACTG 
CAAGGCCAAG CCAAGAATCG AGCAAACCCA AATCGAAGTG AAGTCCAGGC AAACCTTTCT 
CTGTTCCTAG AGGCAGCTAG CGGCTTCTAT ACTCAGTTAT TACAGGAACT GTGTACGGTG 
TTTAATGTAG ATTTGCCATG CCGTGTGAAG TCTTCCCAGT TGGGAATTAT TAGCAATAAA 
CAGACGCACA GCAGCACCAT AGTGAAGCCA CAGTCTAGCT CCTGCTCCTA CATTTGCCAA 
CACTGCCTCG TCCACCTTGG AGACATTGCT CGATACAGAA ATCAGACTAG CCAGGCAGAG 
TCCTACTATA GGCATGCAGC TCAGCTTGTC CCCTCCAATG GTCAGCCTTA CAATCAGTTG 
GCTATCTTAG CTTCTTCCAA AGGAGACCAC CTGACCACAA TTTTCTACTA CTGCAGAAGC 
ATTGCTGTGA AATTCCCTTT CCCAGCTGCC TCCACTAATC TACAAAAAGC ACTTTCTAAA 
GCACTGGAAA GCCGGGATGA GTTGAAAACC AAGTGGGGTG TTTCTGACTT CATCAAGGCC 
TTTATTAAAT TCCACGGTCA TGTATACCTG AGTAAGAGTT TGGAAAAGCT GAGCCCTCTT 
CGAGAGAAGT TGGAAGAACA GTTTAAGAGA CTGCTGTTCC AAAAGGCTTT CAACTCTCAA 
CAGTTAGTTC ATGTCACTGT CATTAACCTG TTTCAACTTC ATCATCTTCG TGACTTTAGC 
AATGAAACAG AGCAACACAG TTACAGCCAA GATGAACAGC TGTGTTGGAC ACAGTTGCTG 
GCCCTCTTTA TGTCTTTTCT CGGCATCCTG TGCAAATGTC CTCTCCAGAA TGATTCTCAG 
GAGTCCAACA ATGCCTACCC CCTTCCTGCA GTCAAGGTCT CCATGGACTG GCTAAGACTC 
CGACCTAGAG TCTTTCAAGA AGCAGTGGTG GATGAAAGAC AGTACATTTG GCCCTGGCTA 
ATTTCTCTTC TAAATAGTTT CCATCCCCGT GAAGATGATC TCTCAAATAC TAATGCCACA 
CCACTTCCAG AGGAGTTTGA ATTACAAGGA TTCTTGGCTT TAAGACCTTC TTTCAGGAAC 
TTGGATTTTT CCAAAGGCCA TCAGGGTATT ACAGGAGACA AAGAGGGTCA ACAACGACGA 
ATACGACAGC AGCGTTTGAT CTCCATAGGG AAATGGATTG CTGATAACCA GCCACGGCTG 
ATTCAGTGTG AAAATGAGGT AGGGAAATTG TTATTTATCA CAGAAATCCC AGAATTAATA 
CTAGAAGACC CCAGTGAAGC CAAAGAGAAC CTCATCCTAC AAGAAACATC TGTGGTAGAG 
TCACTGGCTA CGGATGGGAG CCCAGGACTG AAATCAGTGC TGTCTACAGG CCGAAATCCA 
AGCAACAGCT GTGACTCAGG AGAGAAACCA GTGGTCACCT TTAAAGAGAA CATTAAGCCA 
CGAGAAGTGA ACCAAGGAAG AAGCTTTCCT CCCAAAGAGG TGAGAAGGGA CTGTAGCAAA 
GGCGTAACTG TAACTCAGGA GGACGGACAG AAGGACAGCA GCAAGAGGAG AGCTGAGACC 
AAGAGATGCA CCTTAGGAAA GTTGCAGGAA ACAGGAAAGC AGAGTGTGGC AGTGCAGGTA 
AAATCCCAGA CAGAACTAAG AAAGACTCCA GTGTCTGAAG CCAGGAAAAC GCCTGTCACT 
CAAACTCCAA GTCAAACGAG TAATTCTCAG TTCATCCCCA TCCATCACCC TGGAGCCTTC 
CCTCCTCTTC CCAGCCGACC AGGATTCCCG CCCCCAACAT ATGTTATCCC CCCTCCTGTG 
GCATTTTCTA TGGGCTCAGG TTACACCTTC CCAGCTGGTG TTTCTGTCCC AGGAACCTTT 
CTTCAGTCTA CAGCTCACTC TCCAGCAGGA AACCAGGTGC AAGCTGGGAA ACAGTCCCAC 
ATTCCTTACA GCCAGCAACG GCCCTCTGGA CCAGGGCCAA TGAACCAGGG ACCTCAACAG 
TCACAGCCAC CTTCCCAGCC ACCCCTTACA TCTTTACCAG CTCAGCCAAC AGCACAGTCT 
ACAAGCCAAT TGCAGGTTCA AGCTCTAGCT CAGCAACAGC AATCCCCTAC AAAAGTCATA 
CCAGCTTTGG GGAAAAGCCC GCCTCACCAC TCTGGATTCC AGCAGTATCA ACAGGCAGAT 
GCCTCCAAAC AGCTGTGGAA TCCCCCTCAG GTTCAAAGCC CACTAGGGAA AATTATGCCT 
GTGAAACAGT CCTACTACCT TCAGACCCAA GACCCTATAA AACTGTTTGA GCCGTCATTG 
CAACCTCCTG TAATACAGCA GCAGCCTCTA GAGAAAAAAA TGAAGCCTTT CCCCATGGAG 
CCATATAACC ATAATCCCTC AGAAGTCAAG GTCCCAGAGT TCTACTGGGA TTCTTCCTAC 
AGCATGGCCG ATAACAGAGC AGTAATGGCT CAGCAACCAA ATATGGACCG CAGGAGCAAA 
CGGTCACCTG GAGTCTTCCG TCCAGAACAG GATCCTGTGC CCAGGATGCC ATTTGAGAAA 
TCCTTATTGG AGAAGCCTTC TGAGCTCATG TCACATTCAT CTTCTTTCCT GTCCCTCACG 
GGGTTCTCTG TCAATCAGGA AAGATATCCA AACAGCAGTA TGTTCAATGA AGTGTATGGA 
AAAAACCTGA CAACCAGCTC CAAGGCAGAA CTGAACCCCT CAGTCGCCTC CCAGGAAACA 
TCACTGTACT CCCTTTTTGA AGGGACCCCA TGGTCTCCAT CACTTCCTGC CAGTTCAGAT 
CATTCGACAC CAGCCAGCCA GTCTCCTCAT TCCTCTAACC CAAGCAGCCT GCCCAGTTCT 
CCTCCAACGC ATAACCATAA TTCTGCTCCA TTCTCTAATT TTGGACCCAT TGGGACTCCA 
GATAACAGGG ATAGGCGGCC TGCAGATAGG TGGAAAACTG ATAAACCAGC CATGGGTGGT 
TTTGGTGTTG ATTATCTCTC AGCAACGTCA TCATCTGAGA GCAGTTGGCA TCAGGCTAGC 
ACTCCGAGTG GCACCTGGAC AGGCCACGGC CCCTCCATGG AGGATTCCTC TGCTGTCCTC 
ATGGAAAGCC TAAAGTCTAT CTGGTCCAGT TCCATGATGC ATCCTGGGCC CTCCGCTCTG 
GAGCAGTTGT TAATGCAGCA GAAGCAGAAA CAGCAGCGGG GACAAGGTGC CATGAACCCT 
CCACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030109150.1
CDS	706..4134
Translation	MRTENLKSEEHLKSSNIRQAEVLKAEMTDSKLGPAEVWTSRQALQDLYQKMLVTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRANPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQTHSSTIVKPQSSSCSYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNLQKALSKALESRDELKTKWGVSDFIKAFIKFHGHVYLSKSLEKLSPLREKLEEQFKRLLFQKAFNSQQLVHVTVINLFQLHHLRDFSNETEQHSYSQDEQLCWTQLLALFMSFLGILCKCPLQNDSQESNNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPREDDLSNTNATPLPEEFELQGFLALRPSFRNLDFSKGHQGITGDKEGQQRRIRQQRLISIGKWIADNQPRLIQCENEVGKLLFITEIPELILEDPSEAKENLILQETSVVESLATDGSPGLKSVLSTGRNPSNSCDSGEKPVVTFKENIKPREVNQGRSFPPKEVRRDCSKGVTVTQEDGQKDSSKRRAETKRCTLGKLQETGKQSVAVQVKSQTELRKTPVSEARKTPVTQTPSQTSNSQFIPIHHPGAFPPLPSRPGFPPPTYVIPPPVAFSMGSGYTFPAGVSVPGTFLQSTAHSPAGNQVQAGKQSHIPYSQQRPSGPGPMNQGPQQSQPPSQPPLTSLPAQPTAQSTSQLQVQALAQQQQSPTKVIPALGKSPPHHSGFQQYQQADASKQLWNPPQVQSPLGKIMPVKQSYYLQTQDPIKLFEPSLQPPVIQQQPLEKKMKPFPMEPYNHNPSEVKVPEFYWDSSYSMADNRAVMAQQPNMDRRSKRSPGVFRPEQDPVPRMPFEKSLLEKPSELMSHSSSFLSLTGFSVNQERYPNSSMFNEVYGKNLTTSSKAELNPSVASQETSLYSLFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSAPFSNFGPIGTPDNRDRRPADRWKTDKPAMGGFGVDYLSATSSSESSWHQASTPSGTWTGHGPSMEDSSAVLMESLKSIWSSSMMHPGPSALEQLLMQQKQKQQRGQGAMNPPH

Target ORF information:

RefSeq Version	XM_030253290.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030253290.1

ORF Insert Sequence:

ATGAGGACCG AAAACTTGAA ATCAGAGGAG CATCTGAAAT CAAGTAATAT TAGGCAGGCA 
GAAGTCCTGA AGGCTGAAAT GACAGATTCT AAGCTGGGTC CGGCTGAGGT CTGGACATCC 
AGGCAAGCTC TGCAGGACTT GTACCAGAAA ATGCTAGTTA CTGATCTGGA ATACGCTTTA 
GACAAGAAAG TAGAACAGGA TCTCTGGAAT CATGCCTTTA AGAATCAGAT CACAACACTG 
CAAGGCCAAG CCAAGAATCG AGCAAACCCA AATCGAAGTG AAGTCCAGGC AAACCTTTCT 
CTGTTCCTAG AGGCAGCTAG CGGCTTCTAT ACTCAGTTAT TACAGGAACT GTGTACGGTG 
TTTAATGTAG ATTTGCCATG CCGTGTGAAG TCTTCCCAGT TGGGAATTAT TAGCAATAAA 
CAGACGCACA GCAGCACCAT AGTGAAGCCA CAGTCTAGCT CCTGCTCCTA CATTTGCCAA 
CACTGCCTCG TCCACCTTGG AGACATTGCT CGATACAGAA ATCAGACTAG CCAGGCAGAG 
TCCTACTATA GGCATGCAGC TCAGCTTGTC CCCTCCAATG GTCAGCCTTA CAATCAGTTG 
GCTATCTTAG CTTCTTCCAA AGGAGACCAC CTGACCACAA TTTTCTACTA CTGCAGAAGC 
ATTGCTGTGA AATTCCCTTT CCCAGCTGCC TCCACTAATC TACAAAAAGC ACTTTCTAAA 
GCACTGGAAA GCCGGGATGA GTTGAAAACC AAGTGGGGTG TTTCTGACTT CATCAAGGCC 
TTTATTAAAT TCCACGGTCA TGTATACCTG AGTAAGAGTT TGGAAAAGCT GAGCCCTCTT 
CGAGAGAAGT TGGAAGAACA GTTTAAGAGA CTGCTGTTCC AAAAGGCTTT CAACTCTCAA 
CAGTTAGTTC ATGTCACTGT CATTAACCTG TTTCAACTTC ATCATCTTCG TGACTTTAGC 
AATGAAACAG AGCAACACAG TTACAGCCAA GATGAACAGC TGTGTTGGAC ACAGTTGCTG 
GCCCTCTTTA TGTCTTTTCT CGGCATCCTG TGCAAATGTC CTCTCCAGAA TGATTCTCAG 
GAGTCCAACA ATGCCTACCC CCTTCCTGCA GTCAAGGTCT CCATGGACTG GCTAAGACTC 
CGACCTAGAG TCTTTCAAGA AGCAGTGGTG GATGAAAGAC AGTACATTTG GCCCTGGCTA 
ATTTCTCTTC TAAATAGTTT CCATCCCCGT GAAGATGATC TCTCAAATAC TAATGCCACA 
CCACTTCCAG AGGAGTTTGA ATTACAAGGA TTCTTGGCTT TAAGACCTTC TTTCAGGAAC 
TTGGATTTTT CCAAAGGCCA TCAGGGTATT ACAGGAGACA AAGAGGGTCA ACAACGACGA 
ATACGACAGC AGCGTTTGAT CTCCATAGGG AAATGGATTG CTGATAACCA GCCACGGCTG 
ATTCAGTGTG AAAATGAGGT AGGGAAATTG TTATTTATCA CAGAAATCCC AGAATTAATA 
CTAGAAGACC CCAGTGAAGC CAAAGAGAAC CTCATCCTAC AAGAAACATC TGTGGTAGAG 
TCACTGGCTA CGGATGGGAG CCCAGGACTG AAATCAGTGC TGTCTACAGG CCGAAATCCA 
AGCAACAGCT GTGACTCAGG AGAGAAACCA GTGGTCACCT TTAAAGAGAA CATTAAGCCA 
CGAGAAGTGA ACCAAGGAAG AAGCTTTCCT CCCAAAGAGG TGAGAAGGGA CTGTAGCAAA 
GGCGTAACTG TAACTCAGGA GGACGGACAG AAGGACAGCA GCAAGAGGAG AGCTGAGACC 
AAGAGATGCA CCTTAGGAAA GTTGCAGGAA ACAGGAAAGC AGAGTGTGGC AGTGCAGGTA 
AAATCCCAGA CAGAACTAAG AAAGACTCCA GTGTCTGAAG CCAGGAAAAC GCCTGTCACT 
CAAACTCCAA GTCAAACGAG TAATTCTCAG TTCATCCCCA TCCATCACCC TGGAGCCTTC 
CCTCCTCTTC CCAGCCGACC AGGATTCCCG CCCCCAACAT ATGTTATCCC CCCTCCTGTG 
GCATTTTCTA TGGGCTCAGG TTACACCTTC CCAGCTGGTG TTTCTGTCCC AGGAACCTTT 
CTTCAGTCTA CAGCTCACTC TCCAGCAGGA AACCAGGTGC AAGCTGGGAA ACAGTCCCAC 
ATTCCTTACA GCCAGCAACG GCCCTCTGGA CCAGGGCCAA TGAACCAGGG ACCTCAACAG 
TCACAGCCAC CTTCCCAGCC ACCCCTTACA TCTTTACCAG CTCAGCCAAC AGCACAGTCT 
ACAAGCCAAT TGCAGGTTCA AGCTCTAGCT CAGCAACAGC AATCCCCTAC AAAAGTCATA 
CCAGCTTTGG GGAAAAGCCC GCCTCACCAC TCTGGATTCC AGCAGTATCA ACAGGCAGAT 
GCCTCCAAAC AGCTGTGGAA TCCCCCTCAG GTTCAAAGCC CACTAGGGAA AATTATGCCT 
GTGAAACAGT CCTACTACCT TCAGACCCAA GACCCTATAA AACTGTTTGA GCCGTCATTG 
CAACCTCCTG TAATACAGCA GCAGCCTCTA GAGAAAAAAA TGAAGCCTTT CCCCATGGAG 
CCATATAACC ATAATCCCTC AGAAGTCAAG GTCCCAGAGT TCTACTGGGA TTCTTCCTAC 
AGCATGGCCG ATAACAGAGC AGTAATGGCT CAGCAACCAA ATATGGACCG CAGGAGCAAA 
CGGTCACCTG GAGTCTTCCG TCCAGAACAG GATCCTGTGC CCAGGATGCC ATTTGAGAAA 
TCCTTATTGG AGAAGCCTTC TGAGCTCATG TCACATTCAT CTTCTTTCCT GTCCCTCACG 
GGGTTCTCTG TCAATCAGGA AAGATATCCA AACAGCAGTA TGTTCAATGA AGTGTATGGA 
AAAAACCTGA CAACCAGCTC CAAGGCAGAA CTGAACCCCT CAGTCGCCTC CCAGGAAACA 
TCACTGTACT CCCTTTTTGA AGGGACCCCA TGGTCTCCAT CACTTCCTGC CAGTTCAGAT 
CATTCGACAC CAGCCAGCCA GTCTCCTCAT TCCTCTAACC CAAGCAGCCT GCCCAGTTCT 
CCTCCAACGC ATAACCATAA TTCTGCTCCA TTCTCTAATT TTGGACCCAT TGGGACTCCA 
GATAACAGGG ATAGGCGGCC TGCAGATAGG TGGAAAACTG ATAAACCAGC CATGGGTGGT 
TTTGGTGTTG ATTATCTCTC AGCAACGTCA TCATCTGAGA GCAGTTGGCA TCAGGCTAGC 
ACTCCGAGTG GCACCTGGAC AGGCCACGGC CCCTCCATGG AGGATTCCTC TGCTGTCCTC 
ATGGAAAGCC TAAAGTCTAT CTGGTCCAGT TCCATGATGC ATCCTGGGCC CTCCGCTCTG 
GAGCAGTTGT TAATGCAGCA GAAGCAGAAA CAGCAGCGGG GACAAGGTGC CATGAACCCT 
CCACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Smg7 ( XM_030253292.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030109152.1 Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu28141
Clone ID Related Accession (Same CDS sequence)	XM_030253292.1 , XM_017319979.1
Accession Version	XM_030253292.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3291bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein SMG7 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGACCG AAAACTTGAA ATCAGAGGAG CATCTGAAAT CAAGTAATAT TAGGCAGGCA 
GAAGTCCTGA AGGCTGAAAT GACAGATTCT AAGCTGGGTC CGGCTGAGGT CTGGACATCC 
AGGCAAGCTC TGCAGGACTT GTACCAGAAA ATGCTAGTTA CTGATCTGGA ATACGCTTTA 
GACAAGAAAG TAGAACAGGA TCTCTGGAAT CATGCCTTTA AGAATCAGAT CACAACACTG 
CAAGGCCAAG CCAAGAATCG AGCAAACCCA AATCGAAGTG AAGTCCAGGC AAACCTTTCT 
CTGTTCCTAG AGGCAGCTAG CGGCTTCTAT ACTCAGTTAT TACAGGAACT GTGTACGGTG 
TTTAATGTAG ATTTGCCATG CCGTGTGAAG TCTTCCCAGT TGGGAATTAT TAGCAATAAA 
CAGACGCACA GCAGCACCAT AGTGAAGCCA CAGTCTAGCT CCTGCTCCTA CATTTGCCAA 
CACTGCCTCG TCCACCTTGG AGACATTGCT CGATACAGAA ATCAGACTAG CCAGGCAGAG 
TCCTACTATA GGCATGCAGC TCAGCTTGTC CCCTCCAATG GTCAGCCTTA CAATCAGTTG 
GCTATCTTAG CTTCTTCCAA AGGAGACCAC CTGACCACAA TTTTCTACTA CTGCAGAAGC 
ATTGCTGTGA AATTCCCTTT CCCAGCTGCC TCCACTAATC TACAAAAAGC ACTTTCTAAA 
GCACTGGAAA GCCGGGATGA GTTGAAAACC AAGTGGGGTG TTTCTGACTT CATCAAGGCC 
TTTATTAAAT TCCACGGTCA TGTATACCTG AGTAAGAGTT TGGAAAAGCT GAGCCCTCTT 
CGAGAGAAGT TGGAAGAACA GTTTAAGAGA CTGCTGTTCC AAAAGGCTTT CAACTCTCAA 
CAGTTAGTTC ATGTCACTGT CATTAACCTG TTTCAACTTC ATCATCTTCG TGACTTTAGC 
AATGAAACAG AGCAACACAG TTACAGCCAA GATGAACAGC TGTGTTGGAC ACAGTTGCTG 
GCCCTCTTTA TGTCTTTTCT CGGCATCCTG TGCAAATGTC CTCTCCAGAA TGATTCTCAG 
GAGTCCAACA ATGCCTACCC CCTTCCTGCA GTCAAGGTCT CCATGGACTG GCTAAGACTC 
CGACCTAGAG TCTTTCAAGA AGCAGTGGTG GATGAAAGAC AGTACATTTG GCCCTGGCTA 
ATTTCTCTTC TAAATAGTTT CCATCCCCGT GAAGATGATC TCTCAAATAC TAATGCCACA 
CCACTTCCAG AGGAGTTTGA ATTACAAGGA TTCTTGGCTT TAAGACCTTC TTTCAGGAAC 
TTGGATTTTT CCAAAGGCCA TCAGGGTATT ACAGGAGACA AAGAGGGTCA ACAACGACGA 
ATACGACAGC AGCGTTTGAT CTCCATAGGG AAATGGATTG CTGATAACCA GCCACGGCTG 
ATTCAGTGTG AAAATGAGGT AGGGAAATTG TTATTTATCA CAGAAATCCC AGAATTAATA 
CTAGAAGACC CCAGTGAAGC CAAAGAGAAC CTCATCCTAC AAGAAACATC TGTGGTAGAG 
TCACTGGCTA CGGATGGGAG CCCAGGACTG AAATCAGTGC TGTCTACAGG CCGAAATCCA 
AGCAACAGCT GTGACTCAGG AGAGAAACCA GTGGTCACCT TTAAAGAGAA CATTAAGCCA 
CGAGAAGTGA ACCAAGGAAG AAGCTTTCCT CCCAAAGAGG TAAAATCCCA GACAGAACTA 
AGAAAGACTC CAGTGTCTGA AGCCAGGAAA ACGCCTGTCA CTCAAACTCC AAGTCAAACG 
AGTAATTCTC AGTTCATCCC CATCCATCAC CCTGGAGCCT TCCCTCCTCT TCCCAGCCGA 
CCAGGATTCC CGCCCCCAAC ATATGTTATC CCCCCTCCTG TGGCATTTTC TATGGGCTCA 
GGTTACACCT TCCCAGCTGG TGTTTCTGTC CCAGGAACCT TTCTTCAGTC TACAGCTCAC 
TCTCCAGCAG GAAACCAGGT GCAAGCTGGG AAACAGTCCC ACATTCCTTA CAGCCAGCAA 
CGGCCCTCTG GACCAGGGCC AATGAACCAG GGACCTCAAC AGTCACAGCC ACCTTCCCAG 
CCACCCCTTA CATCTTTACC AGCTCAGCCA ACAGCACAGT CTACAAGCCA ATTGCAGGTT 
CAAGCTCTAG CTCAGCAACA GCAATCCCCT ACAAAAGTCA TACCAGCTTT GGGGAAAAGC 
CCGCCTCACC ACTCTGGATT CCAGCAGTAT CAACAGGCAG ATGCCTCCAA ACAGCTGTGG 
AATCCCCCTC AGGTTCAAAG CCCACTAGGG AAAATTATGC CTGTGAAACA GTCCTACTAC 
CTTCAGACCC AAGACCCTAT AAAACTGTTT GAGCCGTCAT TGCAACCTCC TGTAATACAG 
CAGCAGCCTC TAGAGAAAAA AATGAAGCCT TTCCCCATGG AGCCATATAA CCATAATCCC 
TCAGAAGTCA AGGTCCCAGA GTTCTACTGG GATTCTTCCT ACAGCATGGC CGATAACAGA 
GCAGTAATGG CTCAGCAACC AAATATGGAC CGCAGGAGCA AACGGTCACC TGGAGTCTTC 
CGTCCAGAAC AGGATCCTGT GCCCAGGATG CCATTTGAGA AATCCTTATT GGAGAAGCCT 
TCTGAGCTCA TGTCACATTC ATCTTCTTTC CTGTCCCTCA CGGGGTTCTC TGTCAATCAG 
GAAAGATATC CAAACAGCAG TATGTTCAAT GAAGTGTATG GAAAAAACCT GACAACCAGC 
TCCAAGGCAG AACTGAACCC CTCAGTCGCC TCCCAGGAAA CATCACTGTA CTCCCTTTTT 
GAAGGGACCC CATGGTCTCC ATCACTTCCT GCCAGTTCAG ATCATTCGAC ACCAGCCAGC 
CAGTCTCCTC ATTCCTCTAA CCCAAGCAGC CTGCCCAGTT CTCCTCCAAC GCATAACCAT 
AATTCTGCTC CATTCTCTAA TTTTGGACCC ATTGGGACTC CAGATAACAG GGATAGGCGG 
CCTGCAGATA GGTGGAAAAC TGATAAACCA GCCATGGGTG GTTTTGGTGT TGATTATCTC 
TCAGCAACGT CATCATCTGA GAGCAGTTGG CATCAGGCTA GCACTCCGAG TGGCACCTGG 
ACAGGCCACG GCCCCTCCAT GGAGGATTCC TCTGCTGTCC TCATGGAAAG CCTAAAGTCT 
ATCTGGTCCA GTTCCATGAT GCATCCTGGG CCCTCCGCTC TGGAGCAGTT GTTAATGCAG 
CAGAAGCAGA AACAGCAGCG GGGACAAGGT GCCATGAACC CTCCACACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030109152.1
CDS	705..3995
Translation	MRTENLKSEEHLKSSNIRQAEVLKAEMTDSKLGPAEVWTSRQALQDLYQKMLVTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRANPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQTHSSTIVKPQSSSCSYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNLQKALSKALESRDELKTKWGVSDFIKAFIKFHGHVYLSKSLEKLSPLREKLEEQFKRLLFQKAFNSQQLVHVTVINLFQLHHLRDFSNETEQHSYSQDEQLCWTQLLALFMSFLGILCKCPLQNDSQESNNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPREDDLSNTNATPLPEEFELQGFLALRPSFRNLDFSKGHQGITGDKEGQQRRIRQQRLISIGKWIADNQPRLIQCENEVGKLLFITEIPELILEDPSEAKENLILQETSVVESLATDGSPGLKSVLSTGRNPSNSCDSGEKPVVTFKENIKPREVNQGRSFPPKEVKSQTELRKTPVSEARKTPVTQTPSQTSNSQFIPIHHPGAFPPLPSRPGFPPPTYVIPPPVAFSMGSGYTFPAGVSVPGTFLQSTAHSPAGNQVQAGKQSHIPYSQQRPSGPGPMNQGPQQSQPPSQPPLTSLPAQPTAQSTSQLQVQALAQQQQSPTKVIPALGKSPPHHSGFQQYQQADASKQLWNPPQVQSPLGKIMPVKQSYYLQTQDPIKLFEPSLQPPVIQQQPLEKKMKPFPMEPYNHNPSEVKVPEFYWDSSYSMADNRAVMAQQPNMDRRSKRSPGVFRPEQDPVPRMPFEKSLLEKPSELMSHSSSFLSLTGFSVNQERYPNSSMFNEVYGKNLTTSSKAELNPSVASQETSLYSLFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSAPFSNFGPIGTPDNRDRRPADRWKTDKPAMGGFGVDYLSATSSSESSWHQASTPSGTWTGHGPSMEDSSAVLMESLKSIWSSSMMHPGPSALEQLLMQQKQKQQRGQGAMNPPH

Target ORF information:

RefSeq Version	XM_030253292.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030253292.1

ORF Insert Sequence:

ATGAGGACCG AAAACTTGAA ATCAGAGGAG CATCTGAAAT CAAGTAATAT TAGGCAGGCA 
GAAGTCCTGA AGGCTGAAAT GACAGATTCT AAGCTGGGTC CGGCTGAGGT CTGGACATCC 
AGGCAAGCTC TGCAGGACTT GTACCAGAAA ATGCTAGTTA CTGATCTGGA ATACGCTTTA 
GACAAGAAAG TAGAACAGGA TCTCTGGAAT CATGCCTTTA AGAATCAGAT CACAACACTG 
CAAGGCCAAG CCAAGAATCG AGCAAACCCA AATCGAAGTG AAGTCCAGGC AAACCTTTCT 
CTGTTCCTAG AGGCAGCTAG CGGCTTCTAT ACTCAGTTAT TACAGGAACT GTGTACGGTG 
TTTAATGTAG ATTTGCCATG CCGTGTGAAG TCTTCCCAGT TGGGAATTAT TAGCAATAAA 
CAGACGCACA GCAGCACCAT AGTGAAGCCA CAGTCTAGCT CCTGCTCCTA CATTTGCCAA 
CACTGCCTCG TCCACCTTGG AGACATTGCT CGATACAGAA ATCAGACTAG CCAGGCAGAG 
TCCTACTATA GGCATGCAGC TCAGCTTGTC CCCTCCAATG GTCAGCCTTA CAATCAGTTG 
GCTATCTTAG CTTCTTCCAA AGGAGACCAC CTGACCACAA TTTTCTACTA CTGCAGAAGC 
ATTGCTGTGA AATTCCCTTT CCCAGCTGCC TCCACTAATC TACAAAAAGC ACTTTCTAAA 
GCACTGGAAA GCCGGGATGA GTTGAAAACC AAGTGGGGTG TTTCTGACTT CATCAAGGCC 
TTTATTAAAT TCCACGGTCA TGTATACCTG AGTAAGAGTT TGGAAAAGCT GAGCCCTCTT 
CGAGAGAAGT TGGAAGAACA GTTTAAGAGA CTGCTGTTCC AAAAGGCTTT CAACTCTCAA 
CAGTTAGTTC ATGTCACTGT CATTAACCTG TTTCAACTTC ATCATCTTCG TGACTTTAGC 
AATGAAACAG AGCAACACAG TTACAGCCAA GATGAACAGC TGTGTTGGAC ACAGTTGCTG 
GCCCTCTTTA TGTCTTTTCT CGGCATCCTG TGCAAATGTC CTCTCCAGAA TGATTCTCAG 
GAGTCCAACA ATGCCTACCC CCTTCCTGCA GTCAAGGTCT CCATGGACTG GCTAAGACTC 
CGACCTAGAG TCTTTCAAGA AGCAGTGGTG GATGAAAGAC AGTACATTTG GCCCTGGCTA 
ATTTCTCTTC TAAATAGTTT CCATCCCCGT GAAGATGATC TCTCAAATAC TAATGCCACA 
CCACTTCCAG AGGAGTTTGA ATTACAAGGA TTCTTGGCTT TAAGACCTTC TTTCAGGAAC 
TTGGATTTTT CCAAAGGCCA TCAGGGTATT ACAGGAGACA AAGAGGGTCA ACAACGACGA 
ATACGACAGC AGCGTTTGAT CTCCATAGGG AAATGGATTG CTGATAACCA GCCACGGCTG 
ATTCAGTGTG AAAATGAGGT AGGGAAATTG TTATTTATCA CAGAAATCCC AGAATTAATA 
CTAGAAGACC CCAGTGAAGC CAAAGAGAAC CTCATCCTAC AAGAAACATC TGTGGTAGAG 
TCACTGGCTA CGGATGGGAG CCCAGGACTG AAATCAGTGC TGTCTACAGG CCGAAATCCA 
AGCAACAGCT GTGACTCAGG AGAGAAACCA GTGGTCACCT TTAAAGAGAA CATTAAGCCA 
CGAGAAGTGA ACCAAGGAAG AAGCTTTCCT CCCAAAGAGG TAAAATCCCA GACAGAACTA 
AGAAAGACTC CAGTGTCTGA AGCCAGGAAA ACGCCTGTCA CTCAAACTCC AAGTCAAACG 
AGTAATTCTC AGTTCATCCC CATCCATCAC CCTGGAGCCT TCCCTCCTCT TCCCAGCCGA 
CCAGGATTCC CGCCCCCAAC ATATGTTATC CCCCCTCCTG TGGCATTTTC TATGGGCTCA 
GGTTACACCT TCCCAGCTGG TGTTTCTGTC CCAGGAACCT TTCTTCAGTC TACAGCTCAC 
TCTCCAGCAG GAAACCAGGT GCAAGCTGGG AAACAGTCCC ACATTCCTTA CAGCCAGCAA 
CGGCCCTCTG GACCAGGGCC AATGAACCAG GGACCTCAAC AGTCACAGCC ACCTTCCCAG 
CCACCCCTTA CATCTTTACC AGCTCAGCCA ACAGCACAGT CTACAAGCCA ATTGCAGGTT 
CAAGCTCTAG CTCAGCAACA GCAATCCCCT ACAAAAGTCA TACCAGCTTT GGGGAAAAGC 
CCGCCTCACC ACTCTGGATT CCAGCAGTAT CAACAGGCAG ATGCCTCCAA ACAGCTGTGG 
AATCCCCCTC AGGTTCAAAG CCCACTAGGG AAAATTATGC CTGTGAAACA GTCCTACTAC 
CTTCAGACCC AAGACCCTAT AAAACTGTTT GAGCCGTCAT TGCAACCTCC TGTAATACAG 
CAGCAGCCTC TAGAGAAAAA AATGAAGCCT TTCCCCATGG AGCCATATAA CCATAATCCC 
TCAGAAGTCA AGGTCCCAGA GTTCTACTGG GATTCTTCCT ACAGCATGGC CGATAACAGA 
GCAGTAATGG CTCAGCAACC AAATATGGAC CGCAGGAGCA AACGGTCACC TGGAGTCTTC 
CGTCCAGAAC AGGATCCTGT GCCCAGGATG CCATTTGAGA AATCCTTATT GGAGAAGCCT 
TCTGAGCTCA TGTCACATTC ATCTTCTTTC CTGTCCCTCA CGGGGTTCTC TGTCAATCAG 
GAAAGATATC CAAACAGCAG TATGTTCAAT GAAGTGTATG GAAAAAACCT GACAACCAGC 
TCCAAGGCAG AACTGAACCC CTCAGTCGCC TCCCAGGAAA CATCACTGTA CTCCCTTTTT 
GAAGGGACCC CATGGTCTCC ATCACTTCCT GCCAGTTCAG ATCATTCGAC ACCAGCCAGC 
CAGTCTCCTC ATTCCTCTAA CCCAAGCAGC CTGCCCAGTT CTCCTCCAAC GCATAACCAT 
AATTCTGCTC CATTCTCTAA TTTTGGACCC ATTGGGACTC CAGATAACAG GGATAGGCGG 
CCTGCAGATA GGTGGAAAAC TGATAAACCA GCCATGGGTG GTTTTGGTGT TGATTATCTC 
TCAGCAACGT CATCATCTGA GAGCAGTTGG CATCAGGCTA GCACTCCGAG TGGCACCTGG 
ACAGGCCACG GCCCCTCCAT GGAGGATTCC TCTGCTGTCC TCATGGAAAG CCTAAAGTCT 
ATCTGGTCCA GTTCCATGAT GCATCCTGGG CCCTCCGCTC TGGAGCAGTT GTTAATGCAG 
CAGAAGCAGA AACAGCAGCG GGGACAAGGT GCCATGAACC CTCCACACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Smg7 ( XM_030253294.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030109154.1 Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OMu85123
Clone ID Related Accession (Same CDS sequence)	XM_030253294.1
Accession Version	XM_030253294.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3267bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein SMG7 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCTGC AGAGCGCGCA GTATCTCCGG CAGGCAGAAG TCCTGAAGGC TGAAATGACA 
GATTCTAAGC TGGGTCCGGC TGAGGTCTGG ACATCCAGGC AAGCTCTGCA GGACTTGTAC 
CAGAAAATGC TAGTTACTGA TCTGGAATAC GCTTTAGACA AGAAAGTAGA ACAGGATCTC 
TGGAATCATG CCTTTAAGAA TCAGATCACA ACACTGCAAG GCCAAGCCAA GAATCGAGCA 
AACCCAAATC GAAGTGAAGT CCAGGCAAAC CTTTCTCTGT TCCTAGAGGC AGCTAGCGGC 
TTCTATACTC AGTTATTACA GGAACTGTGT ACGGTGTTTA ATGTAGATTT GCCATGCCGT 
GTGAAGTCTT CCCAGTTGGG AATTATTAGC AATAAACAGA CGCACAGCAG CACCATAGTG 
AAGCCACAGT CTAGCTCCTG CTCCTACATT TGCCAACACT GCCTCGTCCA CCTTGGAGAC 
ATTGCTCGAT ACAGAAATCA GACTAGCCAG GCAGAGTCCT ACTATAGGCA TGCAGCTCAG 
CTTGTCCCCT CCAATGGTCA GCCTTACAAT CAGTTGGCTA TCTTAGCTTC TTCCAAAGGA 
GACCACCTGA CCACAATTTT CTACTACTGC AGAAGCATTG CTGTGAAATT CCCTTTCCCA 
GCTGCCTCCA CTAATCTACA AAAAGCACTT TCTAAAGCAC TGGAAAGCCG GGATGAGTTG 
AAAACCAAGT GGGGTGTTTC TGACTTCATC AAGGCCTTTA TTAAATTCCA CGGTCATGTA 
TACCTGAGTA AGAGTTTGGA AAAGCTGAGC CCTCTTCGAG AGAAGTTGGA AGAACAGTTT 
AAGAGACTGC TGTTCCAAAA GGCTTTCAAC TCTCAACAGT TAGTTCATGT CACTGTCATT 
AACCTGTTTC AACTTCATCA TCTTCGTGAC TTTAGCAATG AAACAGAGCA ACACAGTTAC 
AGCCAAGATG AACAGCTGTG TTGGACACAG TTGCTGGCCC TCTTTATGTC TTTTCTCGGC 
ATCCTGTGCA AATGTCCTCT CCAGAATGAT TCTCAGGAGT CCAACAATGC CTACCCCCTT 
CCTGCAGTCA AGGTCTCCAT GGACTGGCTA AGACTCCGAC CTAGAGTCTT TCAAGAAGCA 
GTGGTGGATG AAAGACAGTA CATTTGGCCC TGGCTAATTT CTCTTCTAAA TAGTTTCCAT 
CCCCGTGAAG ATGATCTCTC AAATACTAAT GCCACACCAC TTCCAGAGGA GTTTGAATTA 
CAAGGATTCT TGGCTTTAAG ACCTTCTTTC AGGAACTTGG ATTTTTCCAA AGGCCATCAG 
GGTATTACAG GAGACAAAGA GGGTCAACAA CGACGAATAC GACAGCAGCG TTTGATCTCC 
ATAGGGAAAT GGATTGCTGA TAACCAGCCA CGGCTGATTC AGTGTGAAAA TGAGGTAGGG 
AAATTGTTAT TTATCACAGA AATCCCAGAA TTAATACTAG AAGACCCCAG TGAAGCCAAA 
GAGAACCTCA TCCTACAAGA AACATCTGTG GTAGAGTCAC TGGCTACGGA TGGGAGCCCA 
GGACTGAAAT CAGTGCTGTC TACAGGCCGA AATCCAAGCA ACAGCTGTGA CTCAGGAGAG 
AAACCAGTGG TCACCTTTAA AGAGAACATT AAGCCACGAG AAGTGAACCA AGGAAGAAGC 
TTTCCTCCCA AAGAGGTAAA ATCCCAGACA GAACTAAGAA AGACTCCAGT GTCTGAAGCC 
AGGAAAACGC CTGTCACTCA AACTCCAAGT CAAACGAGTA ATTCTCAGTT CATCCCCATC 
CATCACCCTG GAGCCTTCCC TCCTCTTCCC AGCCGACCAG GATTCCCGCC CCCAACATAT 
GTTATCCCCC CTCCTGTGGC ATTTTCTATG GGCTCAGGTT ACACCTTCCC AGCTGGTGTT 
TCTGTCCCAG GAACCTTTCT TCAGTCTACA GCTCACTCTC CAGCAGGAAA CCAGGTGCAA 
GCTGGGAAAC AGTCCCACAT TCCTTACAGC CAGCAACGGC CCTCTGGACC AGGGCCAATG 
AACCAGGGAC CTCAACAGTC ACAGCCACCT TCCCAGCCAC CCCTTACATC TTTACCAGCT 
CAGCCAACAG CACAGTCTAC AAGCCAATTG CAGGTTCAAG CTCTAGCTCA GCAACAGCAA 
TCCCCTACAA AAGTCATACC AGCTTTGGGG AAAAGCCCGC CTCACCACTC TGGATTCCAG 
CAGTATCAAC AGGCAGATGC CTCCAAACAG CTGTGGAATC CCCCTCAGGT TCAAAGCCCA 
CTAGGGAAAA TTATGCCTGT GAAACAGTCC TACTACCTTC AGACCCAAGA CCCTATAAAA 
CTGTTTGAGC CGTCATTGCA ACCTCCTGTA ATACAGCAGC AGCCTCTAGA GAAAAAAATG 
AAGCCTTTCC CCATGGAGCC ATATAACCAT AATCCCTCAG AAGTCAAGGT CCCAGAGTTC 
TACTGGGATT CTTCCTACAG CATGGCCGAT AACAGAGCAG TAATGGCTCA GCAACCAAAT 
ATGGACCGCA GGAGCAAACG GTCACCTGGA GTCTTCCGTC CAGAACAGGA TCCTGTGCCC 
AGGATGCCAT TTGAGAAATC CTTATTGGAG AAGCCTTCTG AGCTCATGTC ACATTCATCT 
TCTTTCCTGT CCCTCACGGG GTTCTCTGTC AATCAGGAAA GATATCCAAA CAGCAGTATG 
TTCAATGAAG TGTATGGAAA AAACCTGACA ACCAGCTCCA AGGCAGAACT GAACCCCTCA 
GTCGCCTCCC AGGAAACATC ACTGTACTCC CTTTTTGAAG GGACCCCATG GTCTCCATCA 
CTTCCTGCCA GTTCAGATCA TTCGACACCA GCCAGCCAGT CTCCTCATTC CTCTAACCCA 
AGCAGCCTGC CCAGTTCTCC TCCAACGCAT AACCATAATT CTGCTCCATT CTCTAATTTT 
GGACCCATTG GGACTCCAGA TAACAGGGAT AGGCGGCCTG CAGATAGGTG GAAAACTGAT 
AAACCAGCCA TGGGTGGTTT TGGTGTTGAT TATCTCTCAG CAACGTCATC ATCTGAGAGC 
AGTTGGCATC AGGCTAGCAC TCCGAGTGGC ACCTGGACAG GCCACGGCCC CTCCATGGAG 
GATTCCTCTG CTGTCCTCAT GGAAAGCCTA AAGTCTATCT GGTCCAGTTC CATGATGCAT 
CCTGGGCCCT CCGCTCTGGA GCAGTTGTTA ATGCAGCAGA AGCAGAAACA GCAGCGGGGA 
CAAGGTGCCA TGAACCCTCC ACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030109154.1
CDS	592..3858
Translation	MSLQSAQYLRQAEVLKAEMTDSKLGPAEVWTSRQALQDLYQKMLVTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRANPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQTHSSTIVKPQSSSCSYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNLQKALSKALESRDELKTKWGVSDFIKAFIKFHGHVYLSKSLEKLSPLREKLEEQFKRLLFQKAFNSQQLVHVTVINLFQLHHLRDFSNETEQHSYSQDEQLCWTQLLALFMSFLGILCKCPLQNDSQESNNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPREDDLSNTNATPLPEEFELQGFLALRPSFRNLDFSKGHQGITGDKEGQQRRIRQQRLISIGKWIADNQPRLIQCENEVGKLLFITEIPELILEDPSEAKENLILQETSVVESLATDGSPGLKSVLSTGRNPSNSCDSGEKPVVTFKENIKPREVNQGRSFPPKEVKSQTELRKTPVSEARKTPVTQTPSQTSNSQFIPIHHPGAFPPLPSRPGFPPPTYVIPPPVAFSMGSGYTFPAGVSVPGTFLQSTAHSPAGNQVQAGKQSHIPYSQQRPSGPGPMNQGPQQSQPPSQPPLTSLPAQPTAQSTSQLQVQALAQQQQSPTKVIPALGKSPPHHSGFQQYQQADASKQLWNPPQVQSPLGKIMPVKQSYYLQTQDPIKLFEPSLQPPVIQQQPLEKKMKPFPMEPYNHNPSEVKVPEFYWDSSYSMADNRAVMAQQPNMDRRSKRSPGVFRPEQDPVPRMPFEKSLLEKPSELMSHSSSFLSLTGFSVNQERYPNSSMFNEVYGKNLTTSSKAELNPSVASQETSLYSLFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSAPFSNFGPIGTPDNRDRRPADRWKTDKPAMGGFGVDYLSATSSSESSWHQASTPSGTWTGHGPSMEDSSAVLMESLKSIWSSSMMHPGPSALEQLLMQQKQKQQRGQGAMNPPH

Target ORF information:

RefSeq Version	XM_030253294.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030253294.1

ORF Insert Sequence:

ATGAGCCTGC AGAGCGCGCA GTATCTCCGG CAGGCAGAAG TCCTGAAGGC TGAAATGACA 
GATTCTAAGC TGGGTCCGGC TGAGGTCTGG ACATCCAGGC AAGCTCTGCA GGACTTGTAC 
CAGAAAATGC TAGTTACTGA TCTGGAATAC GCTTTAGACA AGAAAGTAGA ACAGGATCTC 
TGGAATCATG CCTTTAAGAA TCAGATCACA ACACTGCAAG GCCAAGCCAA GAATCGAGCA 
AACCCAAATC GAAGTGAAGT CCAGGCAAAC CTTTCTCTGT TCCTAGAGGC AGCTAGCGGC 
TTCTATACTC AGTTATTACA GGAACTGTGT ACGGTGTTTA ATGTAGATTT GCCATGCCGT 
GTGAAGTCTT CCCAGTTGGG AATTATTAGC AATAAACAGA CGCACAGCAG CACCATAGTG 
AAGCCACAGT CTAGCTCCTG CTCCTACATT TGCCAACACT GCCTCGTCCA CCTTGGAGAC 
ATTGCTCGAT ACAGAAATCA GACTAGCCAG GCAGAGTCCT ACTATAGGCA TGCAGCTCAG 
CTTGTCCCCT CCAATGGTCA GCCTTACAAT CAGTTGGCTA TCTTAGCTTC TTCCAAAGGA 
GACCACCTGA CCACAATTTT CTACTACTGC AGAAGCATTG CTGTGAAATT CCCTTTCCCA 
GCTGCCTCCA CTAATCTACA AAAAGCACTT TCTAAAGCAC TGGAAAGCCG GGATGAGTTG 
AAAACCAAGT GGGGTGTTTC TGACTTCATC AAGGCCTTTA TTAAATTCCA CGGTCATGTA 
TACCTGAGTA AGAGTTTGGA AAAGCTGAGC CCTCTTCGAG AGAAGTTGGA AGAACAGTTT 
AAGAGACTGC TGTTCCAAAA GGCTTTCAAC TCTCAACAGT TAGTTCATGT CACTGTCATT 
AACCTGTTTC AACTTCATCA TCTTCGTGAC TTTAGCAATG AAACAGAGCA ACACAGTTAC 
AGCCAAGATG AACAGCTGTG TTGGACACAG TTGCTGGCCC TCTTTATGTC TTTTCTCGGC 
ATCCTGTGCA AATGTCCTCT CCAGAATGAT TCTCAGGAGT CCAACAATGC CTACCCCCTT 
CCTGCAGTCA AGGTCTCCAT GGACTGGCTA AGACTCCGAC CTAGAGTCTT TCAAGAAGCA 
GTGGTGGATG AAAGACAGTA CATTTGGCCC TGGCTAATTT CTCTTCTAAA TAGTTTCCAT 
CCCCGTGAAG ATGATCTCTC AAATACTAAT GCCACACCAC TTCCAGAGGA GTTTGAATTA 
CAAGGATTCT TGGCTTTAAG ACCTTCTTTC AGGAACTTGG ATTTTTCCAA AGGCCATCAG 
GGTATTACAG GAGACAAAGA GGGTCAACAA CGACGAATAC GACAGCAGCG TTTGATCTCC 
ATAGGGAAAT GGATTGCTGA TAACCAGCCA CGGCTGATTC AGTGTGAAAA TGAGGTAGGG 
AAATTGTTAT TTATCACAGA AATCCCAGAA TTAATACTAG AAGACCCCAG TGAAGCCAAA 
GAGAACCTCA TCCTACAAGA AACATCTGTG GTAGAGTCAC TGGCTACGGA TGGGAGCCCA 
GGACTGAAAT CAGTGCTGTC TACAGGCCGA AATCCAAGCA ACAGCTGTGA CTCAGGAGAG 
AAACCAGTGG TCACCTTTAA AGAGAACATT AAGCCACGAG AAGTGAACCA AGGAAGAAGC 
TTTCCTCCCA AAGAGGTAAA ATCCCAGACA GAACTAAGAA AGACTCCAGT GTCTGAAGCC 
AGGAAAACGC CTGTCACTCA AACTCCAAGT CAAACGAGTA ATTCTCAGTT CATCCCCATC 
CATCACCCTG GAGCCTTCCC TCCTCTTCCC AGCCGACCAG GATTCCCGCC CCCAACATAT 
GTTATCCCCC CTCCTGTGGC ATTTTCTATG GGCTCAGGTT ACACCTTCCC AGCTGGTGTT 
TCTGTCCCAG GAACCTTTCT TCAGTCTACA GCTCACTCTC CAGCAGGAAA CCAGGTGCAA 
GCTGGGAAAC AGTCCCACAT TCCTTACAGC CAGCAACGGC CCTCTGGACC AGGGCCAATG 
AACCAGGGAC CTCAACAGTC ACAGCCACCT TCCCAGCCAC CCCTTACATC TTTACCAGCT 
CAGCCAACAG CACAGTCTAC AAGCCAATTG CAGGTTCAAG CTCTAGCTCA GCAACAGCAA 
TCCCCTACAA AAGTCATACC AGCTTTGGGG AAAAGCCCGC CTCACCACTC TGGATTCCAG 
CAGTATCAAC AGGCAGATGC CTCCAAACAG CTGTGGAATC CCCCTCAGGT TCAAAGCCCA 
CTAGGGAAAA TTATGCCTGT GAAACAGTCC TACTACCTTC AGACCCAAGA CCCTATAAAA 
CTGTTTGAGC CGTCATTGCA ACCTCCTGTA ATACAGCAGC AGCCTCTAGA GAAAAAAATG 
AAGCCTTTCC CCATGGAGCC ATATAACCAT AATCCCTCAG AAGTCAAGGT CCCAGAGTTC 
TACTGGGATT CTTCCTACAG CATGGCCGAT AACAGAGCAG TAATGGCTCA GCAACCAAAT 
ATGGACCGCA GGAGCAAACG GTCACCTGGA GTCTTCCGTC CAGAACAGGA TCCTGTGCCC 
AGGATGCCAT TTGAGAAATC CTTATTGGAG AAGCCTTCTG AGCTCATGTC ACATTCATCT 
TCTTTCCTGT CCCTCACGGG GTTCTCTGTC AATCAGGAAA GATATCCAAA CAGCAGTATG 
TTCAATGAAG TGTATGGAAA AAACCTGACA ACCAGCTCCA AGGCAGAACT GAACCCCTCA 
GTCGCCTCCC AGGAAACATC ACTGTACTCC CTTTTTGAAG GGACCCCATG GTCTCCATCA 
CTTCCTGCCA GTTCAGATCA TTCGACACCA GCCAGCCAGT CTCCTCATTC CTCTAACCCA 
AGCAGCCTGC CCAGTTCTCC TCCAACGCAT AACCATAATT CTGCTCCATT CTCTAATTTT 
GGACCCATTG GGACTCCAGA TAACAGGGAT AGGCGGCCTG CAGATAGGTG GAAAACTGAT 
AAACCAGCCA TGGGTGGTTT TGGTGTTGAT TATCTCTCAG CAACGTCATC ATCTGAGAGC 
AGTTGGCATC AGGCTAGCAC TCCGAGTGGC ACCTGGACAG GCCACGGCCC CTCCATGGAG 
GATTCCTCTG CTGTCCTCAT GGAAAGCCTA AAGTCTATCT GGTCCAGTTC CATGATGCAT 
CCTGGGCCCT CCGCTCTGGA GCAGTTGTTA ATGCAGCAGA AGCAGAAACA GCAGCGGGGA 
CAAGGTGCCA TGAACCCTCC ACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Smg7 ( NM_001160257.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001153729.1 Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant 1, mRNA.

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CloneID	OMu08009
Clone ID Related Accession (Same CDS sequence)	NM_001160257.1 , NM_001160257.2 , XM_006529452.3
Accession Version	NM_001160257.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3441bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-16
Organism	Mus musculus(House mouse)
Product	protein SMG7 isoform 1
Comment	VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK129101.1, AC110038.25 and BC082789.1. Transcript Variant: This variant (1) represents the longest isoform (1). ##Evidence-Data-START## Transcript exon combination :: AK129101.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGGACCG AAAACTTGAA ATCAGAGGAG CATCTGAAAT CAAGTAATAT TAGGCAGGCA 
GAAGTCCTGA AGGCTGAAAT GACAGATTCT AAGCTGGGTC CGGCTGAGGT CTGGACATCC 
AGGCAAGCTC TGCAGGACTT GTACCAGAAA ATGCTAGTTA CTGATCTGGA ATACGCTTTA 
GACAAGAAAG TAGAACAGGA TCTCTGGAAT CATGCCTTTA AGAATCAGAT CACAACACTG 
CAAGGCCAAG CCAAGAATCG AGCAAACCCA AATCGAAGTG AAGTCCAGGC AAACCTTTCT 
CTGTTCCTAG AGGCAGCTAG CGGCTTCTAT ACTCAGTTAT TACAGGAACT GTGTACGGTG 
TTTAATGTAG ATTTGCCATG CCGTGTGAAG TCTTCCCAGT TGGGAATTAT TAGCAATAAA 
CAGACGCACA GCAGCACCAT AGTGAAGCCA CAGTCTAGCT CCTGCTCCTA CATTTGCCAA 
CACTGCCTCG TCCACCTTGG AGACATTGCT CGATACAGAA ATCAGACTAG CCAGGCAGAG 
TCCTACTATA GGCATGCAGC TCAGCTTGTC CCCTCCAATG GTCAGCCTTA CAATCAGTTG 
GCTATCTTAG CTTCTTCCAA AGGAGACCAC CTGACCACAA TTTTCTACTA CTGCAGAAGC 
ATTGCTGTGA AATTCCCTTT CCCAGCTGCC TCCACTAATC TACAAAAAGC ACTTTCTAAA 
GCACTGGAAA GCCGGGATGA GTTGAAAACC AAGTGGGGTG TTTCTGACTT CATCAAGGCC 
TTTATTAAAT TCCACGGTCA TGTATACCTG AGTAAGAGTT TGGAAAAGCT GAGCCCTCTT 
CGAGAGAAGT TGGAAGAACA GTTTAAGAGA CTGCTGTTCC AAAAGGCTTT CAACTCTCAA 
CAGTTAGTTC ATGTCACTGT CATTAACCTG TTTCAACTTC ATCATCTTCG TGACTTTAGC 
AATGAAACAG AGCAACACAG TTACAGCCAA GATGAACAGC TGTGTTGGAC ACAGTTGCTG 
GCCCTCTTTA TGTCTTTTCT CGGCATCCTG TGCAAATGTC CTCTCCAGAA TGATTCTCAG 
GAGTCCAACA ATGCCTACCC CCTTCCTGCA GTCAAGGTCT CCATGGACTG GCTAAGACTC 
CGACCTAGAG TCTTTCAAGA AGCAGTGGTG GATGAAAGAC AGTACATTTG GCCCTGGCTA 
ATTTCTCTTC TAAATAGTTT CCATCCCCGT GAAGATGATC TCTCAAATAC TAATGCCACA 
CCACTTCCAG AGGAGTTTGA ATTACAAGGA TTCTTGGCTT TAAGACCTTC TTTCAGGAAC 
TTGGATTTTT CCAAAGGCCA TCAGGGTATT ACAGGAGACA AAGAGGGTCA ACAACGACGA 
ATACGACAGC AGCGTTTGAT CTCCATAGGG AAATGGATTG CTGATAACCA GCCACGGCTG 
ATTCAGTGTG AAAATGAGGT AGGGAAATTG TTATTTATCA CAGAAATCCC AGAATTAATA 
CTAGAAGACC CCAGTGAAGC CAAAGAGAAC CTCATCCTAC AAGAAACATC TGTGGTAGAG 
TCACTGGCTA CGGATGGGAG CCCAGGACTG AAATCAGTGC TGTCTACAGG CCGAAATCCA 
AGCAACAGCT GTGACTCAGG AGAGAAACCA GTGGTCACCT TTAAAGAGAA CATTAAGCCA 
CGAGAAGTGA ACCAAGGAAG AAGCTTTCCT CCCAAAGAGG TAAAATCCCA GACAGAACTA 
AGAAAGACTC CAGTGTCTGA AGCCAGGAAA ACGCCTGTCA CTCAAACTCC AAGTCAAACG 
AGTAATTCTC AGTTCATCCC CATCCATCAC CCTGGAGCCT TCCCTCCTCT TCCCAGCCGA 
CCAGGATTCC CGCCCCCAAC ATATGTTATC CCCCCTCCTG TGGCATTTTC TATGGGCTCA 
GGTTACACCT TCCCAGCTGG TGTTTCTGTC CCAGGAACCT TTCTTCAGTC TACAGCTCAC 
TCTCCAGCAG GAAACCAGGT GCAAGCTGGG AAACAGTCCC ACATTCCTTA CAGCCAGCAA 
CGGCCCTCTG GACCAGGGCC AATGAACCAG GGACCTCAAC AGTCACAGCC ACCTTCCCAG 
CCACCCCTTA CATCTTTACC AGCTCAGCCA ACAGCACAGT CTACAAGCCA ATTGCAGGTT 
CAAGCTCTAG CTCAGCAACA GCAATCCCCT ACAAAAGTCA TACCAGCTTT GGGGAAAAGC 
CCGCCTCACC ACTCTGGATT CCAGCAGTAT CAACAGGCAG ATGCCTCCAA ACAGCTGTGG 
AATCCCCCTC AGGTTCAAAG CCCACTAGGG AAAATTATGC CTGTGAAACA GTCCTACTAC 
CTTCAGACCC AAGACCCTAT AAAACTGTTT GAGCCGTCAT TGCAACCTCC TGTAATACAG 
CAGCAGCCTC TAGAGAAAAA AATGAAGCCT TTCCCCATGG AGCCATATAA CCATAATCCC 
TCAGAAGTCA AGGTCCCAGA GTTCTACTGG GATTCTTCCT ACAGCATGGC CGATAACAGA 
GCAGTAATGG CTCAGCAACC AAATATGGAC CGCAGGAGCA AACGGTCACC TGGAGTCTTC 
CGTCCAGAAC AGGATCCTGT GCCCAGGATG CCATTTGAGG ACCCCAAGAG CTCCCCTCTG 
CTTCCTCCGG ACCTGTTAAA GAGTCTGGCT GCCTTGGAGG AAGAGGAAGA GCTGATCTTT 
TCTAACCCTC CTGATCTTTA CCCAGCTCTG CTGGGTCCTC TCGCCTCTCT TCCTGGACGA 
AGCCTCTTTA AATCCTTATT GGAGAAGCCT TCTGAGCTCA TGTCACATTC ATCTTCTTTC 
CTGTCCCTCA CGGGGTTCTC TGTCAATCAG GAAAGATATC CAAACAGCAG TATGTTCAAT 
GAAGTGTATG GAAAAAACCT GACAACCAGC TCCAAGGCAG AACTGAACCC CTCAGTCGCC 
TCCCAGGAAA CATCACTGTA CTCCCTTTTT GAAGGGACCC CATGGTCTCC ATCACTTCCT 
GCCAGTTCAG ATCATTCGAC ACCAGCCAGC CAGTCTCCTC ATTCCTCTAA CCCAAGCAGC 
CTGCCCAGTT CTCCTCCAAC GCATAACCAT AATTCTGCTC CATTCTCTAA TTTTGGACCC 
ATTGGGACTC CAGATAACAG GGATAGGCGG CCTGCAGATA GGTGGAAAAC TGATAAACCA 
GCCATGGGTG GTTTTGGTGT TGATTATCTC TCAGCAACGT CATCATCTGA GAGCAGTTGG 
CATCAGGCTA GCACTCCGAG TGGCACCTGG ACAGGCCACG GCCCCTCCAT GGAGGATTCC 
TCTGCTGTCC TCATGGAAAG CCTAAAGTCT ATCTGGTCCA GTTCCATGAT GCATCCTGGG 
CCCTCCGCTC TGGAGCAGTT GTTAATGCAG CAGAAGCAGA AACAGCAGCG GGGACAAGGT 
GCCATGAACC CTCCACACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001153729.1
CDS	76..3516
Translation	MRTENLKSEEHLKSSNIRQAEVLKAEMTDSKLGPAEVWTSRQALQDLYQKMLVTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRANPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQTHSSTIVKPQSSSCSYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNLQKALSKALESRDELKTKWGVSDFIKAFIKFHGHVYLSKSLEKLSPLREKLEEQFKRLLFQKAFNSQQLVHVTVINLFQLHHLRDFSNETEQHSYSQDEQLCWTQLLALFMSFLGILCKCPLQNDSQESNNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPREDDLSNTNATPLPEEFELQGFLALRPSFRNLDFSKGHQGITGDKEGQQRRIRQQRLISIGKWIADNQPRLIQCENEVGKLLFITEIPELILEDPSEAKENLILQETSVVESLATDGSPGLKSVLSTGRNPSNSCDSGEKPVVTFKENIKPREVNQGRSFPPKEVKSQTELRKTPVSEARKTPVTQTPSQTSNSQFIPIHHPGAFPPLPSRPGFPPPTYVIPPPVAFSMGSGYTFPAGVSVPGTFLQSTAHSPAGNQVQAGKQSHIPYSQQRPSGPGPMNQGPQQSQPPSQPPLTSLPAQPTAQSTSQLQVQALAQQQQSPTKVIPALGKSPPHHSGFQQYQQADASKQLWNPPQVQSPLGKIMPVKQSYYLQTQDPIKLFEPSLQPPVIQQQPLEKKMKPFPMEPYNHNPSEVKVPEFYWDSSYSMADNRAVMAQQPNMDRRSKRSPGVFRPEQDPVPRMPFEDPKSSPLLPPDLLKSLAALEEEEELIFSNPPDLYPALLGPLASLPGRSLFKSLLEKPSELMSHSSSFLSLTGFSVNQERYPNSSMFNEVYGKNLTTSSKAELNPSVASQETSLYSLFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSAPFSNFGPIGTPDNRDRRPADRWKTDKPAMGGFGVDYLSATSSSESSWHQASTPSGTWTGHGPSMEDSSAVLMESLKSIWSSSMMHPGPSALEQLLMQQKQKQQRGQGAMNPPH

Target ORF information:

RefSeq Version	NM_001160257.1
Organism	Mus musculus(House mouse)
Definition	Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001160257.1

ORF Insert Sequence:

ATGAGGACCG AAAACTTGAA ATCAGAGGAG CATCTGAAAT CAAGTAATAT TAGGCAGGCA 
GAAGTCCTGA AGGCTGAAAT GACAGATTCT AAGCTGGGTC CGGCTGAGGT CTGGACATCC 
AGGCAAGCTC TGCAGGACTT GTACCAGAAA ATGCTAGTTA CTGATCTGGA ATACGCTTTA 
GACAAGAAAG TAGAACAGGA TCTCTGGAAT CATGCCTTTA AGAATCAGAT CACAACACTG 
CAAGGCCAAG CCAAGAATCG AGCAAACCCA AATCGAAGTG AAGTCCAGGC AAACCTTTCT 
CTGTTCCTAG AGGCAGCTAG CGGCTTCTAT ACTCAGTTAT TACAGGAACT GTGTACGGTG 
TTTAATGTAG ATTTGCCATG CCGTGTGAAG TCTTCCCAGT TGGGAATTAT TAGCAATAAA 
CAGACGCACA GCAGCACCAT AGTGAAGCCA CAGTCTAGCT CCTGCTCCTA CATTTGCCAA 
CACTGCCTCG TCCACCTTGG AGACATTGCT CGATACAGAA ATCAGACTAG CCAGGCAGAG 
TCCTACTATA GGCATGCAGC TCAGCTTGTC CCCTCCAATG GTCAGCCTTA CAATCAGTTG 
GCTATCTTAG CTTCTTCCAA AGGAGACCAC CTGACCACAA TTTTCTACTA CTGCAGAAGC 
ATTGCTGTGA AATTCCCTTT CCCAGCTGCC TCCACTAATC TACAAAAAGC ACTTTCTAAA 
GCACTGGAAA GCCGGGATGA GTTGAAAACC AAGTGGGGTG TTTCTGACTT CATCAAGGCC 
TTTATTAAAT TCCACGGTCA TGTATACCTG AGTAAGAGTT TGGAAAAGCT GAGCCCTCTT 
CGAGAGAAGT TGGAAGAACA GTTTAAGAGA CTGCTGTTCC AAAAGGCTTT CAACTCTCAA 
CAGTTAGTTC ATGTCACTGT CATTAACCTG TTTCAACTTC ATCATCTTCG TGACTTTAGC 
AATGAAACAG AGCAACACAG TTACAGCCAA GATGAACAGC TGTGTTGGAC ACAGTTGCTG 
GCCCTCTTTA TGTCTTTTCT CGGCATCCTG TGCAAATGTC CTCTCCAGAA TGATTCTCAG 
GAGTCCAACA ATGCCTACCC CCTTCCTGCA GTCAAGGTCT CCATGGACTG GCTAAGACTC 
CGACCTAGAG TCTTTCAAGA AGCAGTGGTG GATGAAAGAC AGTACATTTG GCCCTGGCTA 
ATTTCTCTTC TAAATAGTTT CCATCCCCGT GAAGATGATC TCTCAAATAC TAATGCCACA 
CCACTTCCAG AGGAGTTTGA ATTACAAGGA TTCTTGGCTT TAAGACCTTC TTTCAGGAAC 
TTGGATTTTT CCAAAGGCCA TCAGGGTATT ACAGGAGACA AAGAGGGTCA ACAACGACGA 
ATACGACAGC AGCGTTTGAT CTCCATAGGG AAATGGATTG CTGATAACCA GCCACGGCTG 
ATTCAGTGTG AAAATGAGGT AGGGAAATTG TTATTTATCA CAGAAATCCC AGAATTAATA 
CTAGAAGACC CCAGTGAAGC CAAAGAGAAC CTCATCCTAC AAGAAACATC TGTGGTAGAG 
TCACTGGCTA CGGATGGGAG CCCAGGACTG AAATCAGTGC TGTCTACAGG CCGAAATCCA 
AGCAACAGCT GTGACTCAGG AGAGAAACCA GTGGTCACCT TTAAAGAGAA CATTAAGCCA 
CGAGAAGTGA ACCAAGGAAG AAGCTTTCCT CCCAAAGAGG TAAAATCCCA GACAGAACTA 
AGAAAGACTC CAGTGTCTGA AGCCAGGAAA ACGCCTGTCA CTCAAACTCC AAGTCAAACG 
AGTAATTCTC AGTTCATCCC CATCCATCAC CCTGGAGCCT TCCCTCCTCT TCCCAGCCGA 
CCAGGATTCC CGCCCCCAAC ATATGTTATC CCCCCTCCTG TGGCATTTTC TATGGGCTCA 
GGTTACACCT TCCCAGCTGG TGTTTCTGTC CCAGGAACCT TTCTTCAGTC TACAGCTCAC 
TCTCCAGCAG GAAACCAGGT GCAAGCTGGG AAACAGTCCC ACATTCCTTA CAGCCAGCAA 
CGGCCCTCTG GACCAGGGCC AATGAACCAG GGACCTCAAC AGTCACAGCC ACCTTCCCAG 
CCACCCCTTA CATCTTTACC AGCTCAGCCA ACAGCACAGT CTACAAGCCA ATTGCAGGTT 
CAAGCTCTAG CTCAGCAACA GCAATCCCCT ACAAAAGTCA TACCAGCTTT GGGGAAAAGC 
CCGCCTCACC ACTCTGGATT CCAGCAGTAT CAACAGGCAG ATGCCTCCAA ACAGCTGTGG 
AATCCCCCTC AGGTTCAAAG CCCACTAGGG AAAATTATGC CTGTGAAACA GTCCTACTAC 
CTTCAGACCC AAGACCCTAT AAAACTGTTT GAGCCGTCAT TGCAACCTCC TGTAATACAG 
CAGCAGCCTC TAGAGAAAAA AATGAAGCCT TTCCCCATGG AGCCATATAA CCATAATCCC 
TCAGAAGTCA AGGTCCCAGA GTTCTACTGG GATTCTTCCT ACAGCATGGC CGATAACAGA 
GCAGTAATGG CTCAGCAACC AAATATGGAC CGCAGGAGCA AACGGTCACC TGGAGTCTTC 
CGTCCAGAAC AGGATCCTGT GCCCAGGATG CCATTTGAGG ACCCCAAGAG CTCCCCTCTG 
CTTCCTCCGG ACCTGTTAAA GAGTCTGGCT GCCTTGGAGG AAGAGGAAGA GCTGATCTTT 
TCTAACCCTC CTGATCTTTA CCCAGCTCTG CTGGGTCCTC TCGCCTCTCT TCCTGGACGA 
AGCCTCTTTA AATCCTTATT GGAGAAGCCT TCTGAGCTCA TGTCACATTC ATCTTCTTTC 
CTGTCCCTCA CGGGGTTCTC TGTCAATCAG GAAAGATATC CAAACAGCAG TATGTTCAAT 
GAAGTGTATG GAAAAAACCT GACAACCAGC TCCAAGGCAG AACTGAACCC CTCAGTCGCC 
TCCCAGGAAA CATCACTGTA CTCCCTTTTT GAAGGGACCC CATGGTCTCC ATCACTTCCT 
GCCAGTTCAG ATCATTCGAC ACCAGCCAGC CAGTCTCCTC ATTCCTCTAA CCCAAGCAGC 
CTGCCCAGTT CTCCTCCAAC GCATAACCAT AATTCTGCTC CATTCTCTAA TTTTGGACCC 
ATTGGGACTC CAGATAACAG GGATAGGCGG CCTGCAGATA GGTGGAAAAC TGATAAACCA 
GCCATGGGTG GTTTTGGTGT TGATTATCTC TCAGCAACGT CATCATCTGA GAGCAGTTGG 
CATCAGGCTA GCACTCCGAG TGGCACCTGG ACAGGCCACG GCCCCTCCAT GGAGGATTCC 
TCTGCTGTCC TCATGGAAAG CCTAAAGTCT ATCTGGTCCA GTTCCATGAT GCATCCTGGG 
CCCTCCGCTC TGGAGCAGTT GTTAATGCAG CAGAAGCAGA AACAGCAGCG GGGACAAGGT 
GCCATGAACC CTCCACACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Smg7 ( NM_001160257.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001153729.1 Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant 1, mRNA.

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CloneID	OMu08009
Clone ID Related Accession (Same CDS sequence)	NM_001160257.1 , NM_001160257.2 , XM_006529452.3
Accession Version	NM_001160257.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3441bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-21
Organism	Mus musculus(house mouse)
Product	protein SMG7 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC110038.25, AK129101.1 and BC082789.1. On or before Dec 29, 2017 this sequence version replaced XM_006529452.3, NM_001160257.1. Transcript Variant: This variant (1) represents the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660813.209834.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGGACCG AAAACTTGAA ATCAGAGGAG CATCTGAAAT CAAGTAATAT TAGGCAGGCA 
GAAGTCCTGA AGGCTGAAAT GACAGATTCT AAGCTGGGTC CGGCTGAGGT CTGGACATCC 
AGGCAAGCTC TGCAGGACTT GTACCAGAAA ATGCTAGTTA CTGATCTGGA ATACGCTTTA 
GACAAGAAAG TAGAACAGGA TCTCTGGAAT CATGCCTTTA AGAATCAGAT CACAACACTG 
CAAGGCCAAG CCAAGAATCG AGCAAACCCA AATCGAAGTG AAGTCCAGGC AAACCTTTCT 
CTGTTCCTAG AGGCAGCTAG CGGCTTCTAT ACTCAGTTAT TACAGGAACT GTGTACGGTG 
TTTAATGTAG ATTTGCCATG CCGTGTGAAG TCTTCCCAGT TGGGAATTAT TAGCAATAAA 
CAGACGCACA GCAGCACCAT AGTGAAGCCA CAGTCTAGCT CCTGCTCCTA CATTTGCCAA 
CACTGCCTCG TCCACCTTGG AGACATTGCT CGATACAGAA ATCAGACTAG CCAGGCAGAG 
TCCTACTATA GGCATGCAGC TCAGCTTGTC CCCTCCAATG GTCAGCCTTA CAATCAGTTG 
GCTATCTTAG CTTCTTCCAA AGGAGACCAC CTGACCACAA TTTTCTACTA CTGCAGAAGC 
ATTGCTGTGA AATTCCCTTT CCCAGCTGCC TCCACTAATC TACAAAAAGC ACTTTCTAAA 
GCACTGGAAA GCCGGGATGA GTTGAAAACC AAGTGGGGTG TTTCTGACTT CATCAAGGCC 
TTTATTAAAT TCCACGGTCA TGTATACCTG AGTAAGAGTT TGGAAAAGCT GAGCCCTCTT 
CGAGAGAAGT TGGAAGAACA GTTTAAGAGA CTGCTGTTCC AAAAGGCTTT CAACTCTCAA 
CAGTTAGTTC ATGTCACTGT CATTAACCTG TTTCAACTTC ATCATCTTCG TGACTTTAGC 
AATGAAACAG AGCAACACAG TTACAGCCAA GATGAACAGC TGTGTTGGAC ACAGTTGCTG 
GCCCTCTTTA TGTCTTTTCT CGGCATCCTG TGCAAATGTC CTCTCCAGAA TGATTCTCAG 
GAGTCCAACA ATGCCTACCC CCTTCCTGCA GTCAAGGTCT CCATGGACTG GCTAAGACTC 
CGACCTAGAG TCTTTCAAGA AGCAGTGGTG GATGAAAGAC AGTACATTTG GCCCTGGCTA 
ATTTCTCTTC TAAATAGTTT CCATCCCCGT GAAGATGATC TCTCAAATAC TAATGCCACA 
CCACTTCCAG AGGAGTTTGA ATTACAAGGA TTCTTGGCTT TAAGACCTTC TTTCAGGAAC 
TTGGATTTTT CCAAAGGCCA TCAGGGTATT ACAGGAGACA AAGAGGGTCA ACAACGACGA 
ATACGACAGC AGCGTTTGAT CTCCATAGGG AAATGGATTG CTGATAACCA GCCACGGCTG 
ATTCAGTGTG AAAATGAGGT AGGGAAATTG TTATTTATCA CAGAAATCCC AGAATTAATA 
CTAGAAGACC CCAGTGAAGC CAAAGAGAAC CTCATCCTAC AAGAAACATC TGTGGTAGAG 
TCACTGGCTA CGGATGGGAG CCCAGGACTG AAATCAGTGC TGTCTACAGG CCGAAATCCA 
AGCAACAGCT GTGACTCAGG AGAGAAACCA GTGGTCACCT TTAAAGAGAA CATTAAGCCA 
CGAGAAGTGA ACCAAGGAAG AAGCTTTCCT CCCAAAGAGG TAAAATCCCA GACAGAACTA 
AGAAAGACTC CAGTGTCTGA AGCCAGGAAA ACGCCTGTCA CTCAAACTCC AAGTCAAACG 
AGTAATTCTC AGTTCATCCC CATCCATCAC CCTGGAGCCT TCCCTCCTCT TCCCAGCCGA 
CCAGGATTCC CGCCCCCAAC ATATGTTATC CCCCCTCCTG TGGCATTTTC TATGGGCTCA 
GGTTACACCT TCCCAGCTGG TGTTTCTGTC CCAGGAACCT TTCTTCAGTC TACAGCTCAC 
TCTCCAGCAG GAAACCAGGT GCAAGCTGGG AAACAGTCCC ACATTCCTTA CAGCCAGCAA 
CGGCCCTCTG GACCAGGGCC AATGAACCAG GGACCTCAAC AGTCACAGCC ACCTTCCCAG 
CCACCCCTTA CATCTTTACC AGCTCAGCCA ACAGCACAGT CTACAAGCCA ATTGCAGGTT 
CAAGCTCTAG CTCAGCAACA GCAATCCCCT ACAAAAGTCA TACCAGCTTT GGGGAAAAGC 
CCGCCTCACC ACTCTGGATT CCAGCAGTAT CAACAGGCAG ATGCCTCCAA ACAGCTGTGG 
AATCCCCCTC AGGTTCAAAG CCCACTAGGG AAAATTATGC CTGTGAAACA GTCCTACTAC 
CTTCAGACCC AAGACCCTAT AAAACTGTTT GAGCCGTCAT TGCAACCTCC TGTAATACAG 
CAGCAGCCTC TAGAGAAAAA AATGAAGCCT TTCCCCATGG AGCCATATAA CCATAATCCC 
TCAGAAGTCA AGGTCCCAGA GTTCTACTGG GATTCTTCCT ACAGCATGGC CGATAACAGA 
GCAGTAATGG CTCAGCAACC AAATATGGAC CGCAGGAGCA AACGGTCACC TGGAGTCTTC 
CGTCCAGAAC AGGATCCTGT GCCCAGGATG CCATTTGAGG ACCCCAAGAG CTCCCCTCTG 
CTTCCTCCGG ACCTGTTAAA GAGTCTGGCT GCCTTGGAGG AAGAGGAAGA GCTGATCTTT 
TCTAACCCTC CTGATCTTTA CCCAGCTCTG CTGGGTCCTC TCGCCTCTCT TCCTGGACGA 
AGCCTCTTTA AATCCTTATT GGAGAAGCCT TCTGAGCTCA TGTCACATTC ATCTTCTTTC 
CTGTCCCTCA CGGGGTTCTC TGTCAATCAG GAAAGATATC CAAACAGCAG TATGTTCAAT 
GAAGTGTATG GAAAAAACCT GACAACCAGC TCCAAGGCAG AACTGAACCC CTCAGTCGCC 
TCCCAGGAAA CATCACTGTA CTCCCTTTTT GAAGGGACCC CATGGTCTCC ATCACTTCCT 
GCCAGTTCAG ATCATTCGAC ACCAGCCAGC CAGTCTCCTC ATTCCTCTAA CCCAAGCAGC 
CTGCCCAGTT CTCCTCCAAC GCATAACCAT AATTCTGCTC CATTCTCTAA TTTTGGACCC 
ATTGGGACTC CAGATAACAG GGATAGGCGG CCTGCAGATA GGTGGAAAAC TGATAAACCA 
GCCATGGGTG GTTTTGGTGT TGATTATCTC TCAGCAACGT CATCATCTGA GAGCAGTTGG 
CATCAGGCTA GCACTCCGAG TGGCACCTGG ACAGGCCACG GCCCCTCCAT GGAGGATTCC 
TCTGCTGTCC TCATGGAAAG CCTAAAGTCT ATCTGGTCCA GTTCCATGAT GCATCCTGGG 
CCCTCCGCTC TGGAGCAGTT GTTAATGCAG CAGAAGCAGA AACAGCAGCG GGGACAAGGT 
GCCATGAACC CTCCACACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001153729.1
CDS	222..3662
Translation	MRTENLKSEEHLKSSNIRQAEVLKAEMTDSKLGPAEVWTSRQALQDLYQKMLVTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRANPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQTHSSTIVKPQSSSCSYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNLQKALSKALESRDELKTKWGVSDFIKAFIKFHGHVYLSKSLEKLSPLREKLEEQFKRLLFQKAFNSQQLVHVTVINLFQLHHLRDFSNETEQHSYSQDEQLCWTQLLALFMSFLGILCKCPLQNDSQESNNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPREDDLSNTNATPLPEEFELQGFLALRPSFRNLDFSKGHQGITGDKEGQQRRIRQQRLISIGKWIADNQPRLIQCENEVGKLLFITEIPELILEDPSEAKENLILQETSVVESLATDGSPGLKSVLSTGRNPSNSCDSGEKPVVTFKENIKPREVNQGRSFPPKEVKSQTELRKTPVSEARKTPVTQTPSQTSNSQFIPIHHPGAFPPLPSRPGFPPPTYVIPPPVAFSMGSGYTFPAGVSVPGTFLQSTAHSPAGNQVQAGKQSHIPYSQQRPSGPGPMNQGPQQSQPPSQPPLTSLPAQPTAQSTSQLQVQALAQQQQSPTKVIPALGKSPPHHSGFQQYQQADASKQLWNPPQVQSPLGKIMPVKQSYYLQTQDPIKLFEPSLQPPVIQQQPLEKKMKPFPMEPYNHNPSEVKVPEFYWDSSYSMADNRAVMAQQPNMDRRSKRSPGVFRPEQDPVPRMPFEDPKSSPLLPPDLLKSLAALEEEEELIFSNPPDLYPALLGPLASLPGRSLFKSLLEKPSELMSHSSSFLSLTGFSVNQERYPNSSMFNEVYGKNLTTSSKAELNPSVASQETSLYSLFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSAPFSNFGPIGTPDNRDRRPADRWKTDKPAMGGFGVDYLSATSSSESSWHQASTPSGTWTGHGPSMEDSSAVLMESLKSIWSSSMMHPGPSALEQLLMQQKQKQQRGQGAMNPPH

Target ORF information:

RefSeq Version	NM_001160257.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001160257.2

ORF Insert Sequence:

ATGAGGACCG AAAACTTGAA ATCAGAGGAG CATCTGAAAT CAAGTAATAT TAGGCAGGCA 
GAAGTCCTGA AGGCTGAAAT GACAGATTCT AAGCTGGGTC CGGCTGAGGT CTGGACATCC 
AGGCAAGCTC TGCAGGACTT GTACCAGAAA ATGCTAGTTA CTGATCTGGA ATACGCTTTA 
GACAAGAAAG TAGAACAGGA TCTCTGGAAT CATGCCTTTA AGAATCAGAT CACAACACTG 
CAAGGCCAAG CCAAGAATCG AGCAAACCCA AATCGAAGTG AAGTCCAGGC AAACCTTTCT 
CTGTTCCTAG AGGCAGCTAG CGGCTTCTAT ACTCAGTTAT TACAGGAACT GTGTACGGTG 
TTTAATGTAG ATTTGCCATG CCGTGTGAAG TCTTCCCAGT TGGGAATTAT TAGCAATAAA 
CAGACGCACA GCAGCACCAT AGTGAAGCCA CAGTCTAGCT CCTGCTCCTA CATTTGCCAA 
CACTGCCTCG TCCACCTTGG AGACATTGCT CGATACAGAA ATCAGACTAG CCAGGCAGAG 
TCCTACTATA GGCATGCAGC TCAGCTTGTC CCCTCCAATG GTCAGCCTTA CAATCAGTTG 
GCTATCTTAG CTTCTTCCAA AGGAGACCAC CTGACCACAA TTTTCTACTA CTGCAGAAGC 
ATTGCTGTGA AATTCCCTTT CCCAGCTGCC TCCACTAATC TACAAAAAGC ACTTTCTAAA 
GCACTGGAAA GCCGGGATGA GTTGAAAACC AAGTGGGGTG TTTCTGACTT CATCAAGGCC 
TTTATTAAAT TCCACGGTCA TGTATACCTG AGTAAGAGTT TGGAAAAGCT GAGCCCTCTT 
CGAGAGAAGT TGGAAGAACA GTTTAAGAGA CTGCTGTTCC AAAAGGCTTT CAACTCTCAA 
CAGTTAGTTC ATGTCACTGT CATTAACCTG TTTCAACTTC ATCATCTTCG TGACTTTAGC 
AATGAAACAG AGCAACACAG TTACAGCCAA GATGAACAGC TGTGTTGGAC ACAGTTGCTG 
GCCCTCTTTA TGTCTTTTCT CGGCATCCTG TGCAAATGTC CTCTCCAGAA TGATTCTCAG 
GAGTCCAACA ATGCCTACCC CCTTCCTGCA GTCAAGGTCT CCATGGACTG GCTAAGACTC 
CGACCTAGAG TCTTTCAAGA AGCAGTGGTG GATGAAAGAC AGTACATTTG GCCCTGGCTA 
ATTTCTCTTC TAAATAGTTT CCATCCCCGT GAAGATGATC TCTCAAATAC TAATGCCACA 
CCACTTCCAG AGGAGTTTGA ATTACAAGGA TTCTTGGCTT TAAGACCTTC TTTCAGGAAC 
TTGGATTTTT CCAAAGGCCA TCAGGGTATT ACAGGAGACA AAGAGGGTCA ACAACGACGA 
ATACGACAGC AGCGTTTGAT CTCCATAGGG AAATGGATTG CTGATAACCA GCCACGGCTG 
ATTCAGTGTG AAAATGAGGT AGGGAAATTG TTATTTATCA CAGAAATCCC AGAATTAATA 
CTAGAAGACC CCAGTGAAGC CAAAGAGAAC CTCATCCTAC AAGAAACATC TGTGGTAGAG 
TCACTGGCTA CGGATGGGAG CCCAGGACTG AAATCAGTGC TGTCTACAGG CCGAAATCCA 
AGCAACAGCT GTGACTCAGG AGAGAAACCA GTGGTCACCT TTAAAGAGAA CATTAAGCCA 
CGAGAAGTGA ACCAAGGAAG AAGCTTTCCT CCCAAAGAGG TAAAATCCCA GACAGAACTA 
AGAAAGACTC CAGTGTCTGA AGCCAGGAAA ACGCCTGTCA CTCAAACTCC AAGTCAAACG 
AGTAATTCTC AGTTCATCCC CATCCATCAC CCTGGAGCCT TCCCTCCTCT TCCCAGCCGA 
CCAGGATTCC CGCCCCCAAC ATATGTTATC CCCCCTCCTG TGGCATTTTC TATGGGCTCA 
GGTTACACCT TCCCAGCTGG TGTTTCTGTC CCAGGAACCT TTCTTCAGTC TACAGCTCAC 
TCTCCAGCAG GAAACCAGGT GCAAGCTGGG AAACAGTCCC ACATTCCTTA CAGCCAGCAA 
CGGCCCTCTG GACCAGGGCC AATGAACCAG GGACCTCAAC AGTCACAGCC ACCTTCCCAG 
CCACCCCTTA CATCTTTACC AGCTCAGCCA ACAGCACAGT CTACAAGCCA ATTGCAGGTT 
CAAGCTCTAG CTCAGCAACA GCAATCCCCT ACAAAAGTCA TACCAGCTTT GGGGAAAAGC 
CCGCCTCACC ACTCTGGATT CCAGCAGTAT CAACAGGCAG ATGCCTCCAA ACAGCTGTGG 
AATCCCCCTC AGGTTCAAAG CCCACTAGGG AAAATTATGC CTGTGAAACA GTCCTACTAC 
CTTCAGACCC AAGACCCTAT AAAACTGTTT GAGCCGTCAT TGCAACCTCC TGTAATACAG 
CAGCAGCCTC TAGAGAAAAA AATGAAGCCT TTCCCCATGG AGCCATATAA CCATAATCCC 
TCAGAAGTCA AGGTCCCAGA GTTCTACTGG GATTCTTCCT ACAGCATGGC CGATAACAGA 
GCAGTAATGG CTCAGCAACC AAATATGGAC CGCAGGAGCA AACGGTCACC TGGAGTCTTC 
CGTCCAGAAC AGGATCCTGT GCCCAGGATG CCATTTGAGG ACCCCAAGAG CTCCCCTCTG 
CTTCCTCCGG ACCTGTTAAA GAGTCTGGCT GCCTTGGAGG AAGAGGAAGA GCTGATCTTT 
TCTAACCCTC CTGATCTTTA CCCAGCTCTG CTGGGTCCTC TCGCCTCTCT TCCTGGACGA 
AGCCTCTTTA AATCCTTATT GGAGAAGCCT TCTGAGCTCA TGTCACATTC ATCTTCTTTC 
CTGTCCCTCA CGGGGTTCTC TGTCAATCAG GAAAGATATC CAAACAGCAG TATGTTCAAT 
GAAGTGTATG GAAAAAACCT GACAACCAGC TCCAAGGCAG AACTGAACCC CTCAGTCGCC 
TCCCAGGAAA CATCACTGTA CTCCCTTTTT GAAGGGACCC CATGGTCTCC ATCACTTCCT 
GCCAGTTCAG ATCATTCGAC ACCAGCCAGC CAGTCTCCTC ATTCCTCTAA CCCAAGCAGC 
CTGCCCAGTT CTCCTCCAAC GCATAACCAT AATTCTGCTC CATTCTCTAA TTTTGGACCC 
ATTGGGACTC CAGATAACAG GGATAGGCGG CCTGCAGATA GGTGGAAAAC TGATAAACCA 
GCCATGGGTG GTTTTGGTGT TGATTATCTC TCAGCAACGT CATCATCTGA GAGCAGTTGG 
CATCAGGCTA GCACTCCGAG TGGCACCTGG ACAGGCCACG GCCCCTCCAT GGAGGATTCC 
TCTGCTGTCC TCATGGAAAG CCTAAAGTCT ATCTGGTCCA GTTCCATGAT GCATCCTGGG 
CCCTCCGCTC TGGAGCAGTT GTTAATGCAG CAGAAGCAGA AACAGCAGCG GGGACAAGGT 
GCCATGAACC CTCCACACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Smg7 ( XM_006529452.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529515.1 Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant X3, mRNA.

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CloneID	OMu08009
Clone ID Related Accession (Same CDS sequence)	NM_001160257.1 , NM_001160257.2 , XM_006529452.3
Accession Version	XM_006529452.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3441bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(House mouse)
Product	protein SMG7 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006529452.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGACCG AAAACTTGAA ATCAGAGGAG CATCTGAAAT CAAGTAATAT TAGGCAGGCA 
GAAGTCCTGA AGGCTGAAAT GACAGATTCT AAGCTGGGTC CGGCTGAGGT CTGGACATCC 
AGGCAAGCTC TGCAGGACTT GTACCAGAAA ATGCTAGTTA CTGATCTGGA ATACGCTTTA 
GACAAGAAAG TAGAACAGGA TCTCTGGAAT CATGCCTTTA AGAATCAGAT CACAACACTG 
CAAGGCCAAG CCAAGAATCG AGCAAACCCA AATCGAAGTG AAGTCCAGGC AAACCTTTCT 
CTGTTCCTAG AGGCAGCTAG CGGCTTCTAT ACTCAGTTAT TACAGGAACT GTGTACGGTG 
TTTAATGTAG ATTTGCCATG CCGTGTGAAG TCTTCCCAGT TGGGAATTAT TAGCAATAAA 
CAGACGCACA GCAGCACCAT AGTGAAGCCA CAGTCTAGCT CCTGCTCCTA CATTTGCCAA 
CACTGCCTCG TCCACCTTGG AGACATTGCT CGATACAGAA ATCAGACTAG CCAGGCAGAG 
TCCTACTATA GGCATGCAGC TCAGCTTGTC CCCTCCAATG GTCAGCCTTA CAATCAGTTG 
GCTATCTTAG CTTCTTCCAA AGGAGACCAC CTGACCACAA TTTTCTACTA CTGCAGAAGC 
ATTGCTGTGA AATTCCCTTT CCCAGCTGCC TCCACTAATC TACAAAAAGC ACTTTCTAAA 
GCACTGGAAA GCCGGGATGA GTTGAAAACC AAGTGGGGTG TTTCTGACTT CATCAAGGCC 
TTTATTAAAT TCCACGGTCA TGTATACCTG AGTAAGAGTT TGGAAAAGCT GAGCCCTCTT 
CGAGAGAAGT TGGAAGAACA GTTTAAGAGA CTGCTGTTCC AAAAGGCTTT CAACTCTCAA 
CAGTTAGTTC ATGTCACTGT CATTAACCTG TTTCAACTTC ATCATCTTCG TGACTTTAGC 
AATGAAACAG AGCAACACAG TTACAGCCAA GATGAACAGC TGTGTTGGAC ACAGTTGCTG 
GCCCTCTTTA TGTCTTTTCT CGGCATCCTG TGCAAATGTC CTCTCCAGAA TGATTCTCAG 
GAGTCCAACA ATGCCTACCC CCTTCCTGCA GTCAAGGTCT CCATGGACTG GCTAAGACTC 
CGACCTAGAG TCTTTCAAGA AGCAGTGGTG GATGAAAGAC AGTACATTTG GCCCTGGCTA 
ATTTCTCTTC TAAATAGTTT CCATCCCCGT GAAGATGATC TCTCAAATAC TAATGCCACA 
CCACTTCCAG AGGAGTTTGA ATTACAAGGA TTCTTGGCTT TAAGACCTTC TTTCAGGAAC 
TTGGATTTTT CCAAAGGCCA TCAGGGTATT ACAGGAGACA AAGAGGGTCA ACAACGACGA 
ATACGACAGC AGCGTTTGAT CTCCATAGGG AAATGGATTG CTGATAACCA GCCACGGCTG 
ATTCAGTGTG AAAATGAGGT AGGGAAATTG TTATTTATCA CAGAAATCCC AGAATTAATA 
CTAGAAGACC CCAGTGAAGC CAAAGAGAAC CTCATCCTAC AAGAAACATC TGTGGTAGAG 
TCACTGGCTA CGGATGGGAG CCCAGGACTG AAATCAGTGC TGTCTACAGG CCGAAATCCA 
AGCAACAGCT GTGACTCAGG AGAGAAACCA GTGGTCACCT TTAAAGAGAA CATTAAGCCA 
CGAGAAGTGA ACCAAGGAAG AAGCTTTCCT CCCAAAGAGG TAAAATCCCA GACAGAACTA 
AGAAAGACTC CAGTGTCTGA AGCCAGGAAA ACGCCTGTCA CTCAAACTCC AAGTCAAACG 
AGTAATTCTC AGTTCATCCC CATCCATCAC CCTGGAGCCT TCCCTCCTCT TCCCAGCCGA 
CCAGGATTCC CGCCCCCAAC ATATGTTATC CCCCCTCCTG TGGCATTTTC TATGGGCTCA 
GGTTACACCT TCCCAGCTGG TGTTTCTGTC CCAGGAACCT TTCTTCAGTC TACAGCTCAC 
TCTCCAGCAG GAAACCAGGT GCAAGCTGGG AAACAGTCCC ACATTCCTTA CAGCCAGCAA 
CGGCCCTCTG GACCAGGGCC AATGAACCAG GGACCTCAAC AGTCACAGCC ACCTTCCCAG 
CCACCCCTTA CATCTTTACC AGCTCAGCCA ACAGCACAGT CTACAAGCCA ATTGCAGGTT 
CAAGCTCTAG CTCAGCAACA GCAATCCCCT ACAAAAGTCA TACCAGCTTT GGGGAAAAGC 
CCGCCTCACC ACTCTGGATT CCAGCAGTAT CAACAGGCAG ATGCCTCCAA ACAGCTGTGG 
AATCCCCCTC AGGTTCAAAG CCCACTAGGG AAAATTATGC CTGTGAAACA GTCCTACTAC 
CTTCAGACCC AAGACCCTAT AAAACTGTTT GAGCCGTCAT TGCAACCTCC TGTAATACAG 
CAGCAGCCTC TAGAGAAAAA AATGAAGCCT TTCCCCATGG AGCCATATAA CCATAATCCC 
TCAGAAGTCA AGGTCCCAGA GTTCTACTGG GATTCTTCCT ACAGCATGGC CGATAACAGA 
GCAGTAATGG CTCAGCAACC AAATATGGAC CGCAGGAGCA AACGGTCACC TGGAGTCTTC 
CGTCCAGAAC AGGATCCTGT GCCCAGGATG CCATTTGAGG ACCCCAAGAG CTCCCCTCTG 
CTTCCTCCGG ACCTGTTAAA GAGTCTGGCT GCCTTGGAGG AAGAGGAAGA GCTGATCTTT 
TCTAACCCTC CTGATCTTTA CCCAGCTCTG CTGGGTCCTC TCGCCTCTCT TCCTGGACGA 
AGCCTCTTTA AATCCTTATT GGAGAAGCCT TCTGAGCTCA TGTCACATTC ATCTTCTTTC 
CTGTCCCTCA CGGGGTTCTC TGTCAATCAG GAAAGATATC CAAACAGCAG TATGTTCAAT 
GAAGTGTATG GAAAAAACCT GACAACCAGC TCCAAGGCAG AACTGAACCC CTCAGTCGCC 
TCCCAGGAAA CATCACTGTA CTCCCTTTTT GAAGGGACCC CATGGTCTCC ATCACTTCCT 
GCCAGTTCAG ATCATTCGAC ACCAGCCAGC CAGTCTCCTC ATTCCTCTAA CCCAAGCAGC 
CTGCCCAGTT CTCCTCCAAC GCATAACCAT AATTCTGCTC CATTCTCTAA TTTTGGACCC 
ATTGGGACTC CAGATAACAG GGATAGGCGG CCTGCAGATA GGTGGAAAAC TGATAAACCA 
GCCATGGGTG GTTTTGGTGT TGATTATCTC TCAGCAACGT CATCATCTGA GAGCAGTTGG 
CATCAGGCTA GCACTCCGAG TGGCACCTGG ACAGGCCACG GCCCCTCCAT GGAGGATTCC 
TCTGCTGTCC TCATGGAAAG CCTAAAGTCT ATCTGGTCCA GTTCCATGAT GCATCCTGGG 
CCCTCCGCTC TGGAGCAGTT GTTAATGCAG CAGAAGCAGA AACAGCAGCG GGGACAAGGT 
GCCATGAACC CTCCACACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529515.1
CDS	261..3701
Translation	MRTENLKSEEHLKSSNIRQAEVLKAEMTDSKLGPAEVWTSRQALQDLYQKMLVTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRANPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQTHSSTIVKPQSSSCSYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNLQKALSKALESRDELKTKWGVSDFIKAFIKFHGHVYLSKSLEKLSPLREKLEEQFKRLLFQKAFNSQQLVHVTVINLFQLHHLRDFSNETEQHSYSQDEQLCWTQLLALFMSFLGILCKCPLQNDSQESNNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPREDDLSNTNATPLPEEFELQGFLALRPSFRNLDFSKGHQGITGDKEGQQRRIRQQRLISIGKWIADNQPRLIQCENEVGKLLFITEIPELILEDPSEAKENLILQETSVVESLATDGSPGLKSVLSTGRNPSNSCDSGEKPVVTFKENIKPREVNQGRSFPPKEVKSQTELRKTPVSEARKTPVTQTPSQTSNSQFIPIHHPGAFPPLPSRPGFPPPTYVIPPPVAFSMGSGYTFPAGVSVPGTFLQSTAHSPAGNQVQAGKQSHIPYSQQRPSGPGPMNQGPQQSQPPSQPPLTSLPAQPTAQSTSQLQVQALAQQQQSPTKVIPALGKSPPHHSGFQQYQQADASKQLWNPPQVQSPLGKIMPVKQSYYLQTQDPIKLFEPSLQPPVIQQQPLEKKMKPFPMEPYNHNPSEVKVPEFYWDSSYSMADNRAVMAQQPNMDRRSKRSPGVFRPEQDPVPRMPFEDPKSSPLLPPDLLKSLAALEEEEELIFSNPPDLYPALLGPLASLPGRSLFKSLLEKPSELMSHSSSFLSLTGFSVNQERYPNSSMFNEVYGKNLTTSSKAELNPSVASQETSLYSLFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSAPFSNFGPIGTPDNRDRRPADRWKTDKPAMGGFGVDYLSATSSSESSWHQASTPSGTWTGHGPSMEDSSAVLMESLKSIWSSSMMHPGPSALEQLLMQQKQKQQRGQGAMNPPH

Target ORF information:

RefSeq Version	XM_006529452.3
Organism	Mus musculus(House mouse)
Definition	Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006529452.3

ORF Insert Sequence:

ATGAGGACCG AAAACTTGAA ATCAGAGGAG CATCTGAAAT CAAGTAATAT TAGGCAGGCA 
GAAGTCCTGA AGGCTGAAAT GACAGATTCT AAGCTGGGTC CGGCTGAGGT CTGGACATCC 
AGGCAAGCTC TGCAGGACTT GTACCAGAAA ATGCTAGTTA CTGATCTGGA ATACGCTTTA 
GACAAGAAAG TAGAACAGGA TCTCTGGAAT CATGCCTTTA AGAATCAGAT CACAACACTG 
CAAGGCCAAG CCAAGAATCG AGCAAACCCA AATCGAAGTG AAGTCCAGGC AAACCTTTCT 
CTGTTCCTAG AGGCAGCTAG CGGCTTCTAT ACTCAGTTAT TACAGGAACT GTGTACGGTG 
TTTAATGTAG ATTTGCCATG CCGTGTGAAG TCTTCCCAGT TGGGAATTAT TAGCAATAAA 
CAGACGCACA GCAGCACCAT AGTGAAGCCA CAGTCTAGCT CCTGCTCCTA CATTTGCCAA 
CACTGCCTCG TCCACCTTGG AGACATTGCT CGATACAGAA ATCAGACTAG CCAGGCAGAG 
TCCTACTATA GGCATGCAGC TCAGCTTGTC CCCTCCAATG GTCAGCCTTA CAATCAGTTG 
GCTATCTTAG CTTCTTCCAA AGGAGACCAC CTGACCACAA TTTTCTACTA CTGCAGAAGC 
ATTGCTGTGA AATTCCCTTT CCCAGCTGCC TCCACTAATC TACAAAAAGC ACTTTCTAAA 
GCACTGGAAA GCCGGGATGA GTTGAAAACC AAGTGGGGTG TTTCTGACTT CATCAAGGCC 
TTTATTAAAT TCCACGGTCA TGTATACCTG AGTAAGAGTT TGGAAAAGCT GAGCCCTCTT 
CGAGAGAAGT TGGAAGAACA GTTTAAGAGA CTGCTGTTCC AAAAGGCTTT CAACTCTCAA 
CAGTTAGTTC ATGTCACTGT CATTAACCTG TTTCAACTTC ATCATCTTCG TGACTTTAGC 
AATGAAACAG AGCAACACAG TTACAGCCAA GATGAACAGC TGTGTTGGAC ACAGTTGCTG 
GCCCTCTTTA TGTCTTTTCT CGGCATCCTG TGCAAATGTC CTCTCCAGAA TGATTCTCAG 
GAGTCCAACA ATGCCTACCC CCTTCCTGCA GTCAAGGTCT CCATGGACTG GCTAAGACTC 
CGACCTAGAG TCTTTCAAGA AGCAGTGGTG GATGAAAGAC AGTACATTTG GCCCTGGCTA 
ATTTCTCTTC TAAATAGTTT CCATCCCCGT GAAGATGATC TCTCAAATAC TAATGCCACA 
CCACTTCCAG AGGAGTTTGA ATTACAAGGA TTCTTGGCTT TAAGACCTTC TTTCAGGAAC 
TTGGATTTTT CCAAAGGCCA TCAGGGTATT ACAGGAGACA AAGAGGGTCA ACAACGACGA 
ATACGACAGC AGCGTTTGAT CTCCATAGGG AAATGGATTG CTGATAACCA GCCACGGCTG 
ATTCAGTGTG AAAATGAGGT AGGGAAATTG TTATTTATCA CAGAAATCCC AGAATTAATA 
CTAGAAGACC CCAGTGAAGC CAAAGAGAAC CTCATCCTAC AAGAAACATC TGTGGTAGAG 
TCACTGGCTA CGGATGGGAG CCCAGGACTG AAATCAGTGC TGTCTACAGG CCGAAATCCA 
AGCAACAGCT GTGACTCAGG AGAGAAACCA GTGGTCACCT TTAAAGAGAA CATTAAGCCA 
CGAGAAGTGA ACCAAGGAAG AAGCTTTCCT CCCAAAGAGG TAAAATCCCA GACAGAACTA 
AGAAAGACTC CAGTGTCTGA AGCCAGGAAA ACGCCTGTCA CTCAAACTCC AAGTCAAACG 
AGTAATTCTC AGTTCATCCC CATCCATCAC CCTGGAGCCT TCCCTCCTCT TCCCAGCCGA 
CCAGGATTCC CGCCCCCAAC ATATGTTATC CCCCCTCCTG TGGCATTTTC TATGGGCTCA 
GGTTACACCT TCCCAGCTGG TGTTTCTGTC CCAGGAACCT TTCTTCAGTC TACAGCTCAC 
TCTCCAGCAG GAAACCAGGT GCAAGCTGGG AAACAGTCCC ACATTCCTTA CAGCCAGCAA 
CGGCCCTCTG GACCAGGGCC AATGAACCAG GGACCTCAAC AGTCACAGCC ACCTTCCCAG 
CCACCCCTTA CATCTTTACC AGCTCAGCCA ACAGCACAGT CTACAAGCCA ATTGCAGGTT 
CAAGCTCTAG CTCAGCAACA GCAATCCCCT ACAAAAGTCA TACCAGCTTT GGGGAAAAGC 
CCGCCTCACC ACTCTGGATT CCAGCAGTAT CAACAGGCAG ATGCCTCCAA ACAGCTGTGG 
AATCCCCCTC AGGTTCAAAG CCCACTAGGG AAAATTATGC CTGTGAAACA GTCCTACTAC 
CTTCAGACCC AAGACCCTAT AAAACTGTTT GAGCCGTCAT TGCAACCTCC TGTAATACAG 
CAGCAGCCTC TAGAGAAAAA AATGAAGCCT TTCCCCATGG AGCCATATAA CCATAATCCC 
TCAGAAGTCA AGGTCCCAGA GTTCTACTGG GATTCTTCCT ACAGCATGGC CGATAACAGA 
GCAGTAATGG CTCAGCAACC AAATATGGAC CGCAGGAGCA AACGGTCACC TGGAGTCTTC 
CGTCCAGAAC AGGATCCTGT GCCCAGGATG CCATTTGAGG ACCCCAAGAG CTCCCCTCTG 
CTTCCTCCGG ACCTGTTAAA GAGTCTGGCT GCCTTGGAGG AAGAGGAAGA GCTGATCTTT 
TCTAACCCTC CTGATCTTTA CCCAGCTCTG CTGGGTCCTC TCGCCTCTCT TCCTGGACGA 
AGCCTCTTTA AATCCTTATT GGAGAAGCCT TCTGAGCTCA TGTCACATTC ATCTTCTTTC 
CTGTCCCTCA CGGGGTTCTC TGTCAATCAG GAAAGATATC CAAACAGCAG TATGTTCAAT 
GAAGTGTATG GAAAAAACCT GACAACCAGC TCCAAGGCAG AACTGAACCC CTCAGTCGCC 
TCCCAGGAAA CATCACTGTA CTCCCTTTTT GAAGGGACCC CATGGTCTCC ATCACTTCCT 
GCCAGTTCAG ATCATTCGAC ACCAGCCAGC CAGTCTCCTC ATTCCTCTAA CCCAAGCAGC 
CTGCCCAGTT CTCCTCCAAC GCATAACCAT AATTCTGCTC CATTCTCTAA TTTTGGACCC 
ATTGGGACTC CAGATAACAG GGATAGGCGG CCTGCAGATA GGTGGAAAAC TGATAAACCA 
GCCATGGGTG GTTTTGGTGT TGATTATCTC TCAGCAACGT CATCATCTGA GAGCAGTTGG 
CATCAGGCTA GCACTCCGAG TGGCACCTGG ACAGGCCACG GCCCCTCCAT GGAGGATTCC 
TCTGCTGTCC TCATGGAAAG CCTAAAGTCT ATCTGGTCCA GTTCCATGAT GCATCCTGGG 
CCCTCCGCTC TGGAGCAGTT GTTAATGCAG CAGAAGCAGA AACAGCAGCG GGGACAAGGT 
GCCATGAACC CTCCACACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Smg7 ( XM_006529450.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529513.1 Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant X1, mRNA.

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CloneID	OMu28138
Clone ID Related Accession (Same CDS sequence)	XM_006529450.4 , XM_006529450.3
Accession Version	XM_006529450.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3579bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein SMG7 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006529450.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGACCG AAAACTTGAA ATCAGAGGAG CATCTGAAAT CAAGTAATAT TAGGCAGGCA 
GAAGTCCTGA AGGCTGAAAT GACAGATTCT AAGCTGGGTC CGGCTGAGGT CTGGACATCC 
AGGCAAGCTC TGCAGGACTT GTACCAGAAA ATGCTAGTTA CTGATCTGGA ATACGCTTTA 
GACAAGAAAG TAGAACAGGA TCTCTGGAAT CATGCCTTTA AGAATCAGAT CACAACACTG 
CAAGGCCAAG CCAAGAATCG AGCAAACCCA AATCGAAGTG AAGTCCAGGC AAACCTTTCT 
CTGTTCCTAG AGGCAGCTAG CGGCTTCTAT ACTCAGTTAT TACAGGAACT GTGTACGGTG 
TTTAATGTAG ATTTGCCATG CCGTGTGAAG TCTTCCCAGT TGGGAATTAT TAGCAATAAA 
CAGACGCACA GCAGCACCAT AGTGAAGCCA CAGTCTAGCT CCTGCTCCTA CATTTGCCAA 
CACTGCCTCG TCCACCTTGG AGACATTGCT CGATACAGAA ATCAGACTAG CCAGGCAGAG 
TCCTACTATA GGCATGCAGC TCAGCTTGTC CCCTCCAATG GTCAGCCTTA CAATCAGTTG 
GCTATCTTAG CTTCTTCCAA AGGAGACCAC CTGACCACAA TTTTCTACTA CTGCAGAAGC 
ATTGCTGTGA AATTCCCTTT CCCAGCTGCC TCCACTAATC TACAAAAAGC ACTTTCTAAA 
GCACTGGAAA GCCGGGATGA GTTGAAAACC AAGTGGGGTG TTTCTGACTT CATCAAGGCC 
TTTATTAAAT TCCACGGTCA TGTATACCTG AGTAAGAGTT TGGAAAAGCT GAGCCCTCTT 
CGAGAGAAGT TGGAAGAACA GTTTAAGAGA CTGCTGTTCC AAAAGGCTTT CAACTCTCAA 
CAGTTAGTTC ATGTCACTGT CATTAACCTG TTTCAACTTC ATCATCTTCG TGACTTTAGC 
AATGAAACAG AGCAACACAG TTACAGCCAA GATGAACAGC TGTGTTGGAC ACAGTTGCTG 
GCCCTCTTTA TGTCTTTTCT CGGCATCCTG TGCAAATGTC CTCTCCAGAA TGATTCTCAG 
GAGTCCAACA ATGCCTACCC CCTTCCTGCA GTCAAGGTCT CCATGGACTG GCTAAGACTC 
CGACCTAGAG TCTTTCAAGA AGCAGTGGTG GATGAAAGAC AGTACATTTG GCCCTGGCTA 
ATTTCTCTTC TAAATAGTTT CCATCCCCGT GAAGATGATC TCTCAAATAC TAATGCCACA 
CCACTTCCAG AGGAGTTTGA ATTACAAGGA TTCTTGGCTT TAAGACCTTC TTTCAGGAAC 
TTGGATTTTT CCAAAGGCCA TCAGGGTATT ACAGGAGACA AAGAGGGTCA ACAACGACGA 
ATACGACAGC AGCGTTTGAT CTCCATAGGG AAATGGATTG CTGATAACCA GCCACGGCTG 
ATTCAGTGTG AAAATGAGGT AGGGAAATTG TTATTTATCA CAGAAATCCC AGAATTAATA 
CTAGAAGACC CCAGTGAAGC CAAAGAGAAC CTCATCCTAC AAGAAACATC TGTGGTAGAG 
TCACTGGCTA CGGATGGGAG CCCAGGACTG AAATCAGTGC TGTCTACAGG CCGAAATCCA 
AGCAACAGCT GTGACTCAGG AGAGAAACCA GTGGTCACCT TTAAAGAGAA CATTAAGCCA 
CGAGAAGTGA ACCAAGGAAG AAGCTTTCCT CCCAAAGAGG TGAGAAGGGA CTGTAGCAAA 
GGCGTAACTG TAACTCAGGA GGACGGACAG AAGGACAGCA GCAAGAGGAG AGCTGAGACC 
AAGAGATGCA CCTTAGGAAA GTTGCAGGAA ACAGGAAAGC AGAGTGTGGC AGTGCAGGTA 
AAATCCCAGA CAGAACTAAG AAAGACTCCA GTGTCTGAAG CCAGGAAAAC GCCTGTCACT 
CAAACTCCAA GTCAAACGAG TAATTCTCAG TTCATCCCCA TCCATCACCC TGGAGCCTTC 
CCTCCTCTTC CCAGCCGACC AGGATTCCCG CCCCCAACAT ATGTTATCCC CCCTCCTGTG 
GCATTTTCTA TGGGCTCAGG TTACACCTTC CCAGCTGGTG TTTCTGTCCC AGGAACCTTT 
CTTCAGTCTA CAGCTCACTC TCCAGCAGGA AACCAGGTGC AAGCTGGGAA ACAGTCCCAC 
ATTCCTTACA GCCAGCAACG GCCCTCTGGA CCAGGGCCAA TGAACCAGGG ACCTCAACAG 
TCACAGCCAC CTTCCCAGCC ACCCCTTACA TCTTTACCAG CTCAGCCAAC AGCACAGTCT 
ACAAGCCAAT TGCAGGTTCA AGCTCTAGCT CAGCAACAGC AATCCCCTAC AAAAGTCATA 
CCAGCTTTGG GGAAAAGCCC GCCTCACCAC TCTGGATTCC AGCAGTATCA ACAGGCAGAT 
GCCTCCAAAC AGCTGTGGAA TCCCCCTCAG GTTCAAAGCC CACTAGGGAA AATTATGCCT 
GTGAAACAGT CCTACTACCT TCAGACCCAA GACCCTATAA AACTGTTTGA GCCGTCATTG 
CAACCTCCTG TAATACAGCA GCAGCCTCTA GAGAAAAAAA TGAAGCCTTT CCCCATGGAG 
CCATATAACC ATAATCCCTC AGAAGTCAAG GTCCCAGAGT TCTACTGGGA TTCTTCCTAC 
AGCATGGCCG ATAACAGAGC AGTAATGGCT CAGCAACCAA ATATGGACCG CAGGAGCAAA 
CGGTCACCTG GAGTCTTCCG TCCAGAACAG GATCCTGTGC CCAGGATGCC ATTTGAGGAC 
CCCAAGAGCT CCCCTCTGCT TCCTCCGGAC CTGTTAAAGA GTCTGGCTGC CTTGGAGGAA 
GAGGAAGAGC TGATCTTTTC TAACCCTCCT GATCTTTACC CAGCTCTGCT GGGTCCTCTC 
GCCTCTCTTC CTGGACGAAG CCTCTTTAAA TCCTTATTGG AGAAGCCTTC TGAGCTCATG 
TCACATTCAT CTTCTTTCCT GTCCCTCACG GGGTTCTCTG TCAATCAGGA AAGATATCCA 
AACAGCAGTA TGTTCAATGA AGTGTATGGA AAAAACCTGA CAACCAGCTC CAAGGCAGAA 
CTGAACCCCT CAGTCGCCTC CCAGGAAACA TCACTGTACT CCCTTTTTGA AGGGACCCCA 
TGGTCTCCAT CACTTCCTGC CAGTTCAGAT CATTCGACAC CAGCCAGCCA GTCTCCTCAT 
TCCTCTAACC CAAGCAGCCT GCCCAGTTCT CCTCCAACGC ATAACCATAA TTCTGCTCCA 
TTCTCTAATT TTGGACCCAT TGGGACTCCA GATAACAGGG ATAGGCGGCC TGCAGATAGG 
TGGAAAACTG ATAAACCAGC CATGGGTGGT TTTGGTGTTG ATTATCTCTC AGCAACGTCA 
TCATCTGAGA GCAGTTGGCA TCAGGCTAGC ACTCCGAGTG GCACCTGGAC AGGCCACGGC 
CCCTCCATGG AGGATTCCTC TGCTGTCCTC ATGGAAAGCC TAAAGTCTAT CTGGTCCAGT 
TCCATGATGC ATCCTGGGCC CTCCGCTCTG GAGCAGTTGT TAATGCAGCA GAAGCAGAAA 
CAGCAGCGGG GACAAGGTGC CATGAACCCT CCACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529513.1
CDS	261..3839
Translation	MRTENLKSEEHLKSSNIRQAEVLKAEMTDSKLGPAEVWTSRQALQDLYQKMLVTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRANPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQTHSSTIVKPQSSSCSYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNLQKALSKALESRDELKTKWGVSDFIKAFIKFHGHVYLSKSLEKLSPLREKLEEQFKRLLFQKAFNSQQLVHVTVINLFQLHHLRDFSNETEQHSYSQDEQLCWTQLLALFMSFLGILCKCPLQNDSQESNNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPREDDLSNTNATPLPEEFELQGFLALRPSFRNLDFSKGHQGITGDKEGQQRRIRQQRLISIGKWIADNQPRLIQCENEVGKLLFITEIPELILEDPSEAKENLILQETSVVESLATDGSPGLKSVLSTGRNPSNSCDSGEKPVVTFKENIKPREVNQGRSFPPKEVRRDCSKGVTVTQEDGQKDSSKRRAETKRCTLGKLQETGKQSVAVQVKSQTELRKTPVSEARKTPVTQTPSQTSNSQFIPIHHPGAFPPLPSRPGFPPPTYVIPPPVAFSMGSGYTFPAGVSVPGTFLQSTAHSPAGNQVQAGKQSHIPYSQQRPSGPGPMNQGPQQSQPPSQPPLTSLPAQPTAQSTSQLQVQALAQQQQSPTKVIPALGKSPPHHSGFQQYQQADASKQLWNPPQVQSPLGKIMPVKQSYYLQTQDPIKLFEPSLQPPVIQQQPLEKKMKPFPMEPYNHNPSEVKVPEFYWDSSYSMADNRAVMAQQPNMDRRSKRSPGVFRPEQDPVPRMPFEDPKSSPLLPPDLLKSLAALEEEEELIFSNPPDLYPALLGPLASLPGRSLFKSLLEKPSELMSHSSSFLSLTGFSVNQERYPNSSMFNEVYGKNLTTSSKAELNPSVASQETSLYSLFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSAPFSNFGPIGTPDNRDRRPADRWKTDKPAMGGFGVDYLSATSSSESSWHQASTPSGTWTGHGPSMEDSSAVLMESLKSIWSSSMMHPGPSALEQLLMQQKQKQQRGQGAMNPPH

Target ORF information:

RefSeq Version	XM_006529450.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006529450.3

ORF Insert Sequence:

ATGAGGACCG AAAACTTGAA ATCAGAGGAG CATCTGAAAT CAAGTAATAT TAGGCAGGCA 
GAAGTCCTGA AGGCTGAAAT GACAGATTCT AAGCTGGGTC CGGCTGAGGT CTGGACATCC 
AGGCAAGCTC TGCAGGACTT GTACCAGAAA ATGCTAGTTA CTGATCTGGA ATACGCTTTA 
GACAAGAAAG TAGAACAGGA TCTCTGGAAT CATGCCTTTA AGAATCAGAT CACAACACTG 
CAAGGCCAAG CCAAGAATCG AGCAAACCCA AATCGAAGTG AAGTCCAGGC AAACCTTTCT 
CTGTTCCTAG AGGCAGCTAG CGGCTTCTAT ACTCAGTTAT TACAGGAACT GTGTACGGTG 
TTTAATGTAG ATTTGCCATG CCGTGTGAAG TCTTCCCAGT TGGGAATTAT TAGCAATAAA 
CAGACGCACA GCAGCACCAT AGTGAAGCCA CAGTCTAGCT CCTGCTCCTA CATTTGCCAA 
CACTGCCTCG TCCACCTTGG AGACATTGCT CGATACAGAA ATCAGACTAG CCAGGCAGAG 
TCCTACTATA GGCATGCAGC TCAGCTTGTC CCCTCCAATG GTCAGCCTTA CAATCAGTTG 
GCTATCTTAG CTTCTTCCAA AGGAGACCAC CTGACCACAA TTTTCTACTA CTGCAGAAGC 
ATTGCTGTGA AATTCCCTTT CCCAGCTGCC TCCACTAATC TACAAAAAGC ACTTTCTAAA 
GCACTGGAAA GCCGGGATGA GTTGAAAACC AAGTGGGGTG TTTCTGACTT CATCAAGGCC 
TTTATTAAAT TCCACGGTCA TGTATACCTG AGTAAGAGTT TGGAAAAGCT GAGCCCTCTT 
CGAGAGAAGT TGGAAGAACA GTTTAAGAGA CTGCTGTTCC AAAAGGCTTT CAACTCTCAA 
CAGTTAGTTC ATGTCACTGT CATTAACCTG TTTCAACTTC ATCATCTTCG TGACTTTAGC 
AATGAAACAG AGCAACACAG TTACAGCCAA GATGAACAGC TGTGTTGGAC ACAGTTGCTG 
GCCCTCTTTA TGTCTTTTCT CGGCATCCTG TGCAAATGTC CTCTCCAGAA TGATTCTCAG 
GAGTCCAACA ATGCCTACCC CCTTCCTGCA GTCAAGGTCT CCATGGACTG GCTAAGACTC 
CGACCTAGAG TCTTTCAAGA AGCAGTGGTG GATGAAAGAC AGTACATTTG GCCCTGGCTA 
ATTTCTCTTC TAAATAGTTT CCATCCCCGT GAAGATGATC TCTCAAATAC TAATGCCACA 
CCACTTCCAG AGGAGTTTGA ATTACAAGGA TTCTTGGCTT TAAGACCTTC TTTCAGGAAC 
TTGGATTTTT CCAAAGGCCA TCAGGGTATT ACAGGAGACA AAGAGGGTCA ACAACGACGA 
ATACGACAGC AGCGTTTGAT CTCCATAGGG AAATGGATTG CTGATAACCA GCCACGGCTG 
ATTCAGTGTG AAAATGAGGT AGGGAAATTG TTATTTATCA CAGAAATCCC AGAATTAATA 
CTAGAAGACC CCAGTGAAGC CAAAGAGAAC CTCATCCTAC AAGAAACATC TGTGGTAGAG 
TCACTGGCTA CGGATGGGAG CCCAGGACTG AAATCAGTGC TGTCTACAGG CCGAAATCCA 
AGCAACAGCT GTGACTCAGG AGAGAAACCA GTGGTCACCT TTAAAGAGAA CATTAAGCCA 
CGAGAAGTGA ACCAAGGAAG AAGCTTTCCT CCCAAAGAGG TGAGAAGGGA CTGTAGCAAA 
GGCGTAACTG TAACTCAGGA GGACGGACAG AAGGACAGCA GCAAGAGGAG AGCTGAGACC 
AAGAGATGCA CCTTAGGAAA GTTGCAGGAA ACAGGAAAGC AGAGTGTGGC AGTGCAGGTA 
AAATCCCAGA CAGAACTAAG AAAGACTCCA GTGTCTGAAG CCAGGAAAAC GCCTGTCACT 
CAAACTCCAA GTCAAACGAG TAATTCTCAG TTCATCCCCA TCCATCACCC TGGAGCCTTC 
CCTCCTCTTC CCAGCCGACC AGGATTCCCG CCCCCAACAT ATGTTATCCC CCCTCCTGTG 
GCATTTTCTA TGGGCTCAGG TTACACCTTC CCAGCTGGTG TTTCTGTCCC AGGAACCTTT 
CTTCAGTCTA CAGCTCACTC TCCAGCAGGA AACCAGGTGC AAGCTGGGAA ACAGTCCCAC 
ATTCCTTACA GCCAGCAACG GCCCTCTGGA CCAGGGCCAA TGAACCAGGG ACCTCAACAG 
TCACAGCCAC CTTCCCAGCC ACCCCTTACA TCTTTACCAG CTCAGCCAAC AGCACAGTCT 
ACAAGCCAAT TGCAGGTTCA AGCTCTAGCT CAGCAACAGC AATCCCCTAC AAAAGTCATA 
CCAGCTTTGG GGAAAAGCCC GCCTCACCAC TCTGGATTCC AGCAGTATCA ACAGGCAGAT 
GCCTCCAAAC AGCTGTGGAA TCCCCCTCAG GTTCAAAGCC CACTAGGGAA AATTATGCCT 
GTGAAACAGT CCTACTACCT TCAGACCCAA GACCCTATAA AACTGTTTGA GCCGTCATTG 
CAACCTCCTG TAATACAGCA GCAGCCTCTA GAGAAAAAAA TGAAGCCTTT CCCCATGGAG 
CCATATAACC ATAATCCCTC AGAAGTCAAG GTCCCAGAGT TCTACTGGGA TTCTTCCTAC 
AGCATGGCCG ATAACAGAGC AGTAATGGCT CAGCAACCAA ATATGGACCG CAGGAGCAAA 
CGGTCACCTG GAGTCTTCCG TCCAGAACAG GATCCTGTGC CCAGGATGCC ATTTGAGGAC 
CCCAAGAGCT CCCCTCTGCT TCCTCCGGAC CTGTTAAAGA GTCTGGCTGC CTTGGAGGAA 
GAGGAAGAGC TGATCTTTTC TAACCCTCCT GATCTTTACC CAGCTCTGCT GGGTCCTCTC 
GCCTCTCTTC CTGGACGAAG CCTCTTTAAA TCCTTATTGG AGAAGCCTTC TGAGCTCATG 
TCACATTCAT CTTCTTTCCT GTCCCTCACG GGGTTCTCTG TCAATCAGGA AAGATATCCA 
AACAGCAGTA TGTTCAATGA AGTGTATGGA AAAAACCTGA CAACCAGCTC CAAGGCAGAA 
CTGAACCCCT CAGTCGCCTC CCAGGAAACA TCACTGTACT CCCTTTTTGA AGGGACCCCA 
TGGTCTCCAT CACTTCCTGC CAGTTCAGAT CATTCGACAC CAGCCAGCCA GTCTCCTCAT 
TCCTCTAACC CAAGCAGCCT GCCCAGTTCT CCTCCAACGC ATAACCATAA TTCTGCTCCA 
TTCTCTAATT TTGGACCCAT TGGGACTCCA GATAACAGGG ATAGGCGGCC TGCAGATAGG 
TGGAAAACTG ATAAACCAGC CATGGGTGGT TTTGGTGTTG ATTATCTCTC AGCAACGTCA 
TCATCTGAGA GCAGTTGGCA TCAGGCTAGC ACTCCGAGTG GCACCTGGAC AGGCCACGGC 
CCCTCCATGG AGGATTCCTC TGCTGTCCTC ATGGAAAGCC TAAAGTCTAT CTGGTCCAGT 
TCCATGATGC ATCCTGGGCC CTCCGCTCTG GAGCAGTTGT TAATGCAGCA GAAGCAGAAA 
CAGCAGCGGG GACAAGGTGC CATGAACCCT CCACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Smg7 ( XM_006529451.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529514.1 Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu28139
Clone ID Related Accession (Same CDS sequence)	XM_006529451.3 , XM_006529451.4
Accession Version	XM_006529451.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3555bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein SMG7 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006529451.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCTGC AGAGCGCGCA GTATCTCCGG CAGGCAGAAG TCCTGAAGGC TGAAATGACA 
GATTCTAAGC TGGGTCCGGC TGAGGTCTGG ACATCCAGGC AAGCTCTGCA GGACTTGTAC 
CAGAAAATGC TAGTTACTGA TCTGGAATAC GCTTTAGACA AGAAAGTAGA ACAGGATCTC 
TGGAATCATG CCTTTAAGAA TCAGATCACA ACACTGCAAG GCCAAGCCAA GAATCGAGCA 
AACCCAAATC GAAGTGAAGT CCAGGCAAAC CTTTCTCTGT TCCTAGAGGC AGCTAGCGGC 
TTCTATACTC AGTTATTACA GGAACTGTGT ACGGTGTTTA ATGTAGATTT GCCATGCCGT 
GTGAAGTCTT CCCAGTTGGG AATTATTAGC AATAAACAGA CGCACAGCAG CACCATAGTG 
AAGCCACAGT CTAGCTCCTG CTCCTACATT TGCCAACACT GCCTCGTCCA CCTTGGAGAC 
ATTGCTCGAT ACAGAAATCA GACTAGCCAG GCAGAGTCCT ACTATAGGCA TGCAGCTCAG 
CTTGTCCCCT CCAATGGTCA GCCTTACAAT CAGTTGGCTA TCTTAGCTTC TTCCAAAGGA 
GACCACCTGA CCACAATTTT CTACTACTGC AGAAGCATTG CTGTGAAATT CCCTTTCCCA 
GCTGCCTCCA CTAATCTACA AAAAGCACTT TCTAAAGCAC TGGAAAGCCG GGATGAGTTG 
AAAACCAAGT GGGGTGTTTC TGACTTCATC AAGGCCTTTA TTAAATTCCA CGGTCATGTA 
TACCTGAGTA AGAGTTTGGA AAAGCTGAGC CCTCTTCGAG AGAAGTTGGA AGAACAGTTT 
AAGAGACTGC TGTTCCAAAA GGCTTTCAAC TCTCAACAGT TAGTTCATGT CACTGTCATT 
AACCTGTTTC AACTTCATCA TCTTCGTGAC TTTAGCAATG AAACAGAGCA ACACAGTTAC 
AGCCAAGATG AACAGCTGTG TTGGACACAG TTGCTGGCCC TCTTTATGTC TTTTCTCGGC 
ATCCTGTGCA AATGTCCTCT CCAGAATGAT TCTCAGGAGT CCAACAATGC CTACCCCCTT 
CCTGCAGTCA AGGTCTCCAT GGACTGGCTA AGACTCCGAC CTAGAGTCTT TCAAGAAGCA 
GTGGTGGATG AAAGACAGTA CATTTGGCCC TGGCTAATTT CTCTTCTAAA TAGTTTCCAT 
CCCCGTGAAG ATGATCTCTC AAATACTAAT GCCACACCAC TTCCAGAGGA GTTTGAATTA 
CAAGGATTCT TGGCTTTAAG ACCTTCTTTC AGGAACTTGG ATTTTTCCAA AGGCCATCAG 
GGTATTACAG GAGACAAAGA GGGTCAACAA CGACGAATAC GACAGCAGCG TTTGATCTCC 
ATAGGGAAAT GGATTGCTGA TAACCAGCCA CGGCTGATTC AGTGTGAAAA TGAGGTAGGG 
AAATTGTTAT TTATCACAGA AATCCCAGAA TTAATACTAG AAGACCCCAG TGAAGCCAAA 
GAGAACCTCA TCCTACAAGA AACATCTGTG GTAGAGTCAC TGGCTACGGA TGGGAGCCCA 
GGACTGAAAT CAGTGCTGTC TACAGGCCGA AATCCAAGCA ACAGCTGTGA CTCAGGAGAG 
AAACCAGTGG TCACCTTTAA AGAGAACATT AAGCCACGAG AAGTGAACCA AGGAAGAAGC 
TTTCCTCCCA AAGAGGTGAG AAGGGACTGT AGCAAAGGCG TAACTGTAAC TCAGGAGGAC 
GGACAGAAGG ACAGCAGCAA GAGGAGAGCT GAGACCAAGA GATGCACCTT AGGAAAGTTG 
CAGGAAACAG GAAAGCAGAG TGTGGCAGTG CAGGTAAAAT CCCAGACAGA ACTAAGAAAG 
ACTCCAGTGT CTGAAGCCAG GAAAACGCCT GTCACTCAAA CTCCAAGTCA AACGAGTAAT 
TCTCAGTTCA TCCCCATCCA TCACCCTGGA GCCTTCCCTC CTCTTCCCAG CCGACCAGGA 
TTCCCGCCCC CAACATATGT TATCCCCCCT CCTGTGGCAT TTTCTATGGG CTCAGGTTAC 
ACCTTCCCAG CTGGTGTTTC TGTCCCAGGA ACCTTTCTTC AGTCTACAGC TCACTCTCCA 
GCAGGAAACC AGGTGCAAGC TGGGAAACAG TCCCACATTC CTTACAGCCA GCAACGGCCC 
TCTGGACCAG GGCCAATGAA CCAGGGACCT CAACAGTCAC AGCCACCTTC CCAGCCACCC 
CTTACATCTT TACCAGCTCA GCCAACAGCA CAGTCTACAA GCCAATTGCA GGTTCAAGCT 
CTAGCTCAGC AACAGCAATC CCCTACAAAA GTCATACCAG CTTTGGGGAA AAGCCCGCCT 
CACCACTCTG GATTCCAGCA GTATCAACAG GCAGATGCCT CCAAACAGCT GTGGAATCCC 
CCTCAGGTTC AAAGCCCACT AGGGAAAATT ATGCCTGTGA AACAGTCCTA CTACCTTCAG 
ACCCAAGACC CTATAAAACT GTTTGAGCCG TCATTGCAAC CTCCTGTAAT ACAGCAGCAG 
CCTCTAGAGA AAAAAATGAA GCCTTTCCCC ATGGAGCCAT ATAACCATAA TCCCTCAGAA 
GTCAAGGTCC CAGAGTTCTA CTGGGATTCT TCCTACAGCA TGGCCGATAA CAGAGCAGTA 
ATGGCTCAGC AACCAAATAT GGACCGCAGG AGCAAACGGT CACCTGGAGT CTTCCGTCCA 
GAACAGGATC CTGTGCCCAG GATGCCATTT GAGGACCCCA AGAGCTCCCC TCTGCTTCCT 
CCGGACCTGT TAAAGAGTCT GGCTGCCTTG GAGGAAGAGG AAGAGCTGAT CTTTTCTAAC 
CCTCCTGATC TTTACCCAGC TCTGCTGGGT CCTCTCGCCT CTCTTCCTGG ACGAAGCCTC 
TTTAAATCCT TATTGGAGAA GCCTTCTGAG CTCATGTCAC ATTCATCTTC TTTCCTGTCC 
CTCACGGGGT TCTCTGTCAA TCAGGAAAGA TATCCAAACA GCAGTATGTT CAATGAAGTG 
TATGGAAAAA ACCTGACAAC CAGCTCCAAG GCAGAACTGA ACCCCTCAGT CGCCTCCCAG 
GAAACATCAC TGTACTCCCT TTTTGAAGGG ACCCCATGGT CTCCATCACT TCCTGCCAGT 
TCAGATCATT CGACACCAGC CAGCCAGTCT CCTCATTCCT CTAACCCAAG CAGCCTGCCC 
AGTTCTCCTC CAACGCATAA CCATAATTCT GCTCCATTCT CTAATTTTGG ACCCATTGGG 
ACTCCAGATA ACAGGGATAG GCGGCCTGCA GATAGGTGGA AAACTGATAA ACCAGCCATG 
GGTGGTTTTG GTGTTGATTA TCTCTCAGCA ACGTCATCAT CTGAGAGCAG TTGGCATCAG 
GCTAGCACTC CGAGTGGCAC CTGGACAGGC CACGGCCCCT CCATGGAGGA TTCCTCTGCT 
GTCCTCATGG AAAGCCTAAA GTCTATCTGG TCCAGTTCCA TGATGCATCC TGGGCCCTCC 
GCTCTGGAGC AGTTGTTAAT GCAGCAGAAG CAGAAACAGC AGCGGGGACA AGGTGCCATG 
AACCCTCCAC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529514.1
CDS	148..3702
Translation	MSLQSAQYLRQAEVLKAEMTDSKLGPAEVWTSRQALQDLYQKMLVTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRANPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQTHSSTIVKPQSSSCSYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNLQKALSKALESRDELKTKWGVSDFIKAFIKFHGHVYLSKSLEKLSPLREKLEEQFKRLLFQKAFNSQQLVHVTVINLFQLHHLRDFSNETEQHSYSQDEQLCWTQLLALFMSFLGILCKCPLQNDSQESNNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPREDDLSNTNATPLPEEFELQGFLALRPSFRNLDFSKGHQGITGDKEGQQRRIRQQRLISIGKWIADNQPRLIQCENEVGKLLFITEIPELILEDPSEAKENLILQETSVVESLATDGSPGLKSVLSTGRNPSNSCDSGEKPVVTFKENIKPREVNQGRSFPPKEVRRDCSKGVTVTQEDGQKDSSKRRAETKRCTLGKLQETGKQSVAVQVKSQTELRKTPVSEARKTPVTQTPSQTSNSQFIPIHHPGAFPPLPSRPGFPPPTYVIPPPVAFSMGSGYTFPAGVSVPGTFLQSTAHSPAGNQVQAGKQSHIPYSQQRPSGPGPMNQGPQQSQPPSQPPLTSLPAQPTAQSTSQLQVQALAQQQQSPTKVIPALGKSPPHHSGFQQYQQADASKQLWNPPQVQSPLGKIMPVKQSYYLQTQDPIKLFEPSLQPPVIQQQPLEKKMKPFPMEPYNHNPSEVKVPEFYWDSSYSMADNRAVMAQQPNMDRRSKRSPGVFRPEQDPVPRMPFEDPKSSPLLPPDLLKSLAALEEEEELIFSNPPDLYPALLGPLASLPGRSLFKSLLEKPSELMSHSSSFLSLTGFSVNQERYPNSSMFNEVYGKNLTTSSKAELNPSVASQETSLYSLFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSAPFSNFGPIGTPDNRDRRPADRWKTDKPAMGGFGVDYLSATSSSESSWHQASTPSGTWTGHGPSMEDSSAVLMESLKSIWSSSMMHPGPSALEQLLMQQKQKQQRGQGAMNPPH

Target ORF information:

RefSeq Version	XM_006529451.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006529451.3

ORF Insert Sequence:

ATGAGCCTGC AGAGCGCGCA GTATCTCCGG CAGGCAGAAG TCCTGAAGGC TGAAATGACA 
GATTCTAAGC TGGGTCCGGC TGAGGTCTGG ACATCCAGGC AAGCTCTGCA GGACTTGTAC 
CAGAAAATGC TAGTTACTGA TCTGGAATAC GCTTTAGACA AGAAAGTAGA ACAGGATCTC 
TGGAATCATG CCTTTAAGAA TCAGATCACA ACACTGCAAG GCCAAGCCAA GAATCGAGCA 
AACCCAAATC GAAGTGAAGT CCAGGCAAAC CTTTCTCTGT TCCTAGAGGC AGCTAGCGGC 
TTCTATACTC AGTTATTACA GGAACTGTGT ACGGTGTTTA ATGTAGATTT GCCATGCCGT 
GTGAAGTCTT CCCAGTTGGG AATTATTAGC AATAAACAGA CGCACAGCAG CACCATAGTG 
AAGCCACAGT CTAGCTCCTG CTCCTACATT TGCCAACACT GCCTCGTCCA CCTTGGAGAC 
ATTGCTCGAT ACAGAAATCA GACTAGCCAG GCAGAGTCCT ACTATAGGCA TGCAGCTCAG 
CTTGTCCCCT CCAATGGTCA GCCTTACAAT CAGTTGGCTA TCTTAGCTTC TTCCAAAGGA 
GACCACCTGA CCACAATTTT CTACTACTGC AGAAGCATTG CTGTGAAATT CCCTTTCCCA 
GCTGCCTCCA CTAATCTACA AAAAGCACTT TCTAAAGCAC TGGAAAGCCG GGATGAGTTG 
AAAACCAAGT GGGGTGTTTC TGACTTCATC AAGGCCTTTA TTAAATTCCA CGGTCATGTA 
TACCTGAGTA AGAGTTTGGA AAAGCTGAGC CCTCTTCGAG AGAAGTTGGA AGAACAGTTT 
AAGAGACTGC TGTTCCAAAA GGCTTTCAAC TCTCAACAGT TAGTTCATGT CACTGTCATT 
AACCTGTTTC AACTTCATCA TCTTCGTGAC TTTAGCAATG AAACAGAGCA ACACAGTTAC 
AGCCAAGATG AACAGCTGTG TTGGACACAG TTGCTGGCCC TCTTTATGTC TTTTCTCGGC 
ATCCTGTGCA AATGTCCTCT CCAGAATGAT TCTCAGGAGT CCAACAATGC CTACCCCCTT 
CCTGCAGTCA AGGTCTCCAT GGACTGGCTA AGACTCCGAC CTAGAGTCTT TCAAGAAGCA 
GTGGTGGATG AAAGACAGTA CATTTGGCCC TGGCTAATTT CTCTTCTAAA TAGTTTCCAT 
CCCCGTGAAG ATGATCTCTC AAATACTAAT GCCACACCAC TTCCAGAGGA GTTTGAATTA 
CAAGGATTCT TGGCTTTAAG ACCTTCTTTC AGGAACTTGG ATTTTTCCAA AGGCCATCAG 
GGTATTACAG GAGACAAAGA GGGTCAACAA CGACGAATAC GACAGCAGCG TTTGATCTCC 
ATAGGGAAAT GGATTGCTGA TAACCAGCCA CGGCTGATTC AGTGTGAAAA TGAGGTAGGG 
AAATTGTTAT TTATCACAGA AATCCCAGAA TTAATACTAG AAGACCCCAG TGAAGCCAAA 
GAGAACCTCA TCCTACAAGA AACATCTGTG GTAGAGTCAC TGGCTACGGA TGGGAGCCCA 
GGACTGAAAT CAGTGCTGTC TACAGGCCGA AATCCAAGCA ACAGCTGTGA CTCAGGAGAG 
AAACCAGTGG TCACCTTTAA AGAGAACATT AAGCCACGAG AAGTGAACCA AGGAAGAAGC 
TTTCCTCCCA AAGAGGTGAG AAGGGACTGT AGCAAAGGCG TAACTGTAAC TCAGGAGGAC 
GGACAGAAGG ACAGCAGCAA GAGGAGAGCT GAGACCAAGA GATGCACCTT AGGAAAGTTG 
CAGGAAACAG GAAAGCAGAG TGTGGCAGTG CAGGTAAAAT CCCAGACAGA ACTAAGAAAG 
ACTCCAGTGT CTGAAGCCAG GAAAACGCCT GTCACTCAAA CTCCAAGTCA AACGAGTAAT 
TCTCAGTTCA TCCCCATCCA TCACCCTGGA GCCTTCCCTC CTCTTCCCAG CCGACCAGGA 
TTCCCGCCCC CAACATATGT TATCCCCCCT CCTGTGGCAT TTTCTATGGG CTCAGGTTAC 
ACCTTCCCAG CTGGTGTTTC TGTCCCAGGA ACCTTTCTTC AGTCTACAGC TCACTCTCCA 
GCAGGAAACC AGGTGCAAGC TGGGAAACAG TCCCACATTC CTTACAGCCA GCAACGGCCC 
TCTGGACCAG GGCCAATGAA CCAGGGACCT CAACAGTCAC AGCCACCTTC CCAGCCACCC 
CTTACATCTT TACCAGCTCA GCCAACAGCA CAGTCTACAA GCCAATTGCA GGTTCAAGCT 
CTAGCTCAGC AACAGCAATC CCCTACAAAA GTCATACCAG CTTTGGGGAA AAGCCCGCCT 
CACCACTCTG GATTCCAGCA GTATCAACAG GCAGATGCCT CCAAACAGCT GTGGAATCCC 
CCTCAGGTTC AAAGCCCACT AGGGAAAATT ATGCCTGTGA AACAGTCCTA CTACCTTCAG 
ACCCAAGACC CTATAAAACT GTTTGAGCCG TCATTGCAAC CTCCTGTAAT ACAGCAGCAG 
CCTCTAGAGA AAAAAATGAA GCCTTTCCCC ATGGAGCCAT ATAACCATAA TCCCTCAGAA 
GTCAAGGTCC CAGAGTTCTA CTGGGATTCT TCCTACAGCA TGGCCGATAA CAGAGCAGTA 
ATGGCTCAGC AACCAAATAT GGACCGCAGG AGCAAACGGT CACCTGGAGT CTTCCGTCCA 
GAACAGGATC CTGTGCCCAG GATGCCATTT GAGGACCCCA AGAGCTCCCC TCTGCTTCCT 
CCGGACCTGT TAAAGAGTCT GGCTGCCTTG GAGGAAGAGG AAGAGCTGAT CTTTTCTAAC 
CCTCCTGATC TTTACCCAGC TCTGCTGGGT CCTCTCGCCT CTCTTCCTGG ACGAAGCCTC 
TTTAAATCCT TATTGGAGAA GCCTTCTGAG CTCATGTCAC ATTCATCTTC TTTCCTGTCC 
CTCACGGGGT TCTCTGTCAA TCAGGAAAGA TATCCAAACA GCAGTATGTT CAATGAAGTG 
TATGGAAAAA ACCTGACAAC CAGCTCCAAG GCAGAACTGA ACCCCTCAGT CGCCTCCCAG 
GAAACATCAC TGTACTCCCT TTTTGAAGGG ACCCCATGGT CTCCATCACT TCCTGCCAGT 
TCAGATCATT CGACACCAGC CAGCCAGTCT CCTCATTCCT CTAACCCAAG CAGCCTGCCC 
AGTTCTCCTC CAACGCATAA CCATAATTCT GCTCCATTCT CTAATTTTGG ACCCATTGGG 
ACTCCAGATA ACAGGGATAG GCGGCCTGCA GATAGGTGGA AAACTGATAA ACCAGCCATG 
GGTGGTTTTG GTGTTGATTA TCTCTCAGCA ACGTCATCAT CTGAGAGCAG TTGGCATCAG 
GCTAGCACTC CGAGTGGCAC CTGGACAGGC CACGGCCCCT CCATGGAGGA TTCCTCTGCT 
GTCCTCATGG AAAGCCTAAA GTCTATCTGG TCCAGTTCCA TGATGCATCC TGGGCCCTCC 
GCTCTGGAGC AGTTGTTAAT GCAGCAGAAG CAGAAACAGC AGCGGGGACA AGGTGCCATG 
AACCCTCCAC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Smg7 ( XM_017319978.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017175467.1 Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant X4, mRNA.

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CloneID	OMu28140
Clone ID Related Accession (Same CDS sequence)	XM_017319978.1 , XM_030253290.1
Accession Version	XM_017319978.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3429bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein SMG7 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGACCG AAAACTTGAA ATCAGAGGAG CATCTGAAAT CAAGTAATAT TAGGCAGGCA 
GAAGTCCTGA AGGCTGAAAT GACAGATTCT AAGCTGGGTC CGGCTGAGGT CTGGACATCC 
AGGCAAGCTC TGCAGGACTT GTACCAGAAA ATGCTAGTTA CTGATCTGGA ATACGCTTTA 
GACAAGAAAG TAGAACAGGA TCTCTGGAAT CATGCCTTTA AGAATCAGAT CACAACACTG 
CAAGGCCAAG CCAAGAATCG AGCAAACCCA AATCGAAGTG AAGTCCAGGC AAACCTTTCT 
CTGTTCCTAG AGGCAGCTAG CGGCTTCTAT ACTCAGTTAT TACAGGAACT GTGTACGGTG 
TTTAATGTAG ATTTGCCATG CCGTGTGAAG TCTTCCCAGT TGGGAATTAT TAGCAATAAA 
CAGACGCACA GCAGCACCAT AGTGAAGCCA CAGTCTAGCT CCTGCTCCTA CATTTGCCAA 
CACTGCCTCG TCCACCTTGG AGACATTGCT CGATACAGAA ATCAGACTAG CCAGGCAGAG 
TCCTACTATA GGCATGCAGC TCAGCTTGTC CCCTCCAATG GTCAGCCTTA CAATCAGTTG 
GCTATCTTAG CTTCTTCCAA AGGAGACCAC CTGACCACAA TTTTCTACTA CTGCAGAAGC 
ATTGCTGTGA AATTCCCTTT CCCAGCTGCC TCCACTAATC TACAAAAAGC ACTTTCTAAA 
GCACTGGAAA GCCGGGATGA GTTGAAAACC AAGTGGGGTG TTTCTGACTT CATCAAGGCC 
TTTATTAAAT TCCACGGTCA TGTATACCTG AGTAAGAGTT TGGAAAAGCT GAGCCCTCTT 
CGAGAGAAGT TGGAAGAACA GTTTAAGAGA CTGCTGTTCC AAAAGGCTTT CAACTCTCAA 
CAGTTAGTTC ATGTCACTGT CATTAACCTG TTTCAACTTC ATCATCTTCG TGACTTTAGC 
AATGAAACAG AGCAACACAG TTACAGCCAA GATGAACAGC TGTGTTGGAC ACAGTTGCTG 
GCCCTCTTTA TGTCTTTTCT CGGCATCCTG TGCAAATGTC CTCTCCAGAA TGATTCTCAG 
GAGTCCAACA ATGCCTACCC CCTTCCTGCA GTCAAGGTCT CCATGGACTG GCTAAGACTC 
CGACCTAGAG TCTTTCAAGA AGCAGTGGTG GATGAAAGAC AGTACATTTG GCCCTGGCTA 
ATTTCTCTTC TAAATAGTTT CCATCCCCGT GAAGATGATC TCTCAAATAC TAATGCCACA 
CCACTTCCAG AGGAGTTTGA ATTACAAGGA TTCTTGGCTT TAAGACCTTC TTTCAGGAAC 
TTGGATTTTT CCAAAGGCCA TCAGGGTATT ACAGGAGACA AAGAGGGTCA ACAACGACGA 
ATACGACAGC AGCGTTTGAT CTCCATAGGG AAATGGATTG CTGATAACCA GCCACGGCTG 
ATTCAGTGTG AAAATGAGGT AGGGAAATTG TTATTTATCA CAGAAATCCC AGAATTAATA 
CTAGAAGACC CCAGTGAAGC CAAAGAGAAC CTCATCCTAC AAGAAACATC TGTGGTAGAG 
TCACTGGCTA CGGATGGGAG CCCAGGACTG AAATCAGTGC TGTCTACAGG CCGAAATCCA 
AGCAACAGCT GTGACTCAGG AGAGAAACCA GTGGTCACCT TTAAAGAGAA CATTAAGCCA 
CGAGAAGTGA ACCAAGGAAG AAGCTTTCCT CCCAAAGAGG TGAGAAGGGA CTGTAGCAAA 
GGCGTAACTG TAACTCAGGA GGACGGACAG AAGGACAGCA GCAAGAGGAG AGCTGAGACC 
AAGAGATGCA CCTTAGGAAA GTTGCAGGAA ACAGGAAAGC AGAGTGTGGC AGTGCAGGTA 
AAATCCCAGA CAGAACTAAG AAAGACTCCA GTGTCTGAAG CCAGGAAAAC GCCTGTCACT 
CAAACTCCAA GTCAAACGAG TAATTCTCAG TTCATCCCCA TCCATCACCC TGGAGCCTTC 
CCTCCTCTTC CCAGCCGACC AGGATTCCCG CCCCCAACAT ATGTTATCCC CCCTCCTGTG 
GCATTTTCTA TGGGCTCAGG TTACACCTTC CCAGCTGGTG TTTCTGTCCC AGGAACCTTT 
CTTCAGTCTA CAGCTCACTC TCCAGCAGGA AACCAGGTGC AAGCTGGGAA ACAGTCCCAC 
ATTCCTTACA GCCAGCAACG GCCCTCTGGA CCAGGGCCAA TGAACCAGGG ACCTCAACAG 
TCACAGCCAC CTTCCCAGCC ACCCCTTACA TCTTTACCAG CTCAGCCAAC AGCACAGTCT 
ACAAGCCAAT TGCAGGTTCA AGCTCTAGCT CAGCAACAGC AATCCCCTAC AAAAGTCATA 
CCAGCTTTGG GGAAAAGCCC GCCTCACCAC TCTGGATTCC AGCAGTATCA ACAGGCAGAT 
GCCTCCAAAC AGCTGTGGAA TCCCCCTCAG GTTCAAAGCC CACTAGGGAA AATTATGCCT 
GTGAAACAGT CCTACTACCT TCAGACCCAA GACCCTATAA AACTGTTTGA GCCGTCATTG 
CAACCTCCTG TAATACAGCA GCAGCCTCTA GAGAAAAAAA TGAAGCCTTT CCCCATGGAG 
CCATATAACC ATAATCCCTC AGAAGTCAAG GTCCCAGAGT TCTACTGGGA TTCTTCCTAC 
AGCATGGCCG ATAACAGAGC AGTAATGGCT CAGCAACCAA ATATGGACCG CAGGAGCAAA 
CGGTCACCTG GAGTCTTCCG TCCAGAACAG GATCCTGTGC CCAGGATGCC ATTTGAGAAA 
TCCTTATTGG AGAAGCCTTC TGAGCTCATG TCACATTCAT CTTCTTTCCT GTCCCTCACG 
GGGTTCTCTG TCAATCAGGA AAGATATCCA AACAGCAGTA TGTTCAATGA AGTGTATGGA 
AAAAACCTGA CAACCAGCTC CAAGGCAGAA CTGAACCCCT CAGTCGCCTC CCAGGAAACA 
TCACTGTACT CCCTTTTTGA AGGGACCCCA TGGTCTCCAT CACTTCCTGC CAGTTCAGAT 
CATTCGACAC CAGCCAGCCA GTCTCCTCAT TCCTCTAACC CAAGCAGCCT GCCCAGTTCT 
CCTCCAACGC ATAACCATAA TTCTGCTCCA TTCTCTAATT TTGGACCCAT TGGGACTCCA 
GATAACAGGG ATAGGCGGCC TGCAGATAGG TGGAAAACTG ATAAACCAGC CATGGGTGGT 
TTTGGTGTTG ATTATCTCTC AGCAACGTCA TCATCTGAGA GCAGTTGGCA TCAGGCTAGC 
ACTCCGAGTG GCACCTGGAC AGGCCACGGC CCCTCCATGG AGGATTCCTC TGCTGTCCTC 
ATGGAAAGCC TAAAGTCTAT CTGGTCCAGT TCCATGATGC ATCCTGGGCC CTCCGCTCTG 
GAGCAGTTGT TAATGCAGCA GAAGCAGAAA CAGCAGCGGG GACAAGGTGC CATGAACCCT 
CCACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017175467.1
CDS	85..3513
Translation	MRTENLKSEEHLKSSNIRQAEVLKAEMTDSKLGPAEVWTSRQALQDLYQKMLVTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRANPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQTHSSTIVKPQSSSCSYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNLQKALSKALESRDELKTKWGVSDFIKAFIKFHGHVYLSKSLEKLSPLREKLEEQFKRLLFQKAFNSQQLVHVTVINLFQLHHLRDFSNETEQHSYSQDEQLCWTQLLALFMSFLGILCKCPLQNDSQESNNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPREDDLSNTNATPLPEEFELQGFLALRPSFRNLDFSKGHQGITGDKEGQQRRIRQQRLISIGKWIADNQPRLIQCENEVGKLLFITEIPELILEDPSEAKENLILQETSVVESLATDGSPGLKSVLSTGRNPSNSCDSGEKPVVTFKENIKPREVNQGRSFPPKEVRRDCSKGVTVTQEDGQKDSSKRRAETKRCTLGKLQETGKQSVAVQVKSQTELRKTPVSEARKTPVTQTPSQTSNSQFIPIHHPGAFPPLPSRPGFPPPTYVIPPPVAFSMGSGYTFPAGVSVPGTFLQSTAHSPAGNQVQAGKQSHIPYSQQRPSGPGPMNQGPQQSQPPSQPPLTSLPAQPTAQSTSQLQVQALAQQQQSPTKVIPALGKSPPHHSGFQQYQQADASKQLWNPPQVQSPLGKIMPVKQSYYLQTQDPIKLFEPSLQPPVIQQQPLEKKMKPFPMEPYNHNPSEVKVPEFYWDSSYSMADNRAVMAQQPNMDRRSKRSPGVFRPEQDPVPRMPFEKSLLEKPSELMSHSSSFLSLTGFSVNQERYPNSSMFNEVYGKNLTTSSKAELNPSVASQETSLYSLFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSAPFSNFGPIGTPDNRDRRPADRWKTDKPAMGGFGVDYLSATSSSESSWHQASTPSGTWTGHGPSMEDSSAVLMESLKSIWSSSMMHPGPSALEQLLMQQKQKQQRGQGAMNPPH

Target ORF information:

RefSeq Version	XM_017319978.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319978.1

ORF Insert Sequence:

ATGAGGACCG AAAACTTGAA ATCAGAGGAG CATCTGAAAT CAAGTAATAT TAGGCAGGCA 
GAAGTCCTGA AGGCTGAAAT GACAGATTCT AAGCTGGGTC CGGCTGAGGT CTGGACATCC 
AGGCAAGCTC TGCAGGACTT GTACCAGAAA ATGCTAGTTA CTGATCTGGA ATACGCTTTA 
GACAAGAAAG TAGAACAGGA TCTCTGGAAT CATGCCTTTA AGAATCAGAT CACAACACTG 
CAAGGCCAAG CCAAGAATCG AGCAAACCCA AATCGAAGTG AAGTCCAGGC AAACCTTTCT 
CTGTTCCTAG AGGCAGCTAG CGGCTTCTAT ACTCAGTTAT TACAGGAACT GTGTACGGTG 
TTTAATGTAG ATTTGCCATG CCGTGTGAAG TCTTCCCAGT TGGGAATTAT TAGCAATAAA 
CAGACGCACA GCAGCACCAT AGTGAAGCCA CAGTCTAGCT CCTGCTCCTA CATTTGCCAA 
CACTGCCTCG TCCACCTTGG AGACATTGCT CGATACAGAA ATCAGACTAG CCAGGCAGAG 
TCCTACTATA GGCATGCAGC TCAGCTTGTC CCCTCCAATG GTCAGCCTTA CAATCAGTTG 
GCTATCTTAG CTTCTTCCAA AGGAGACCAC CTGACCACAA TTTTCTACTA CTGCAGAAGC 
ATTGCTGTGA AATTCCCTTT CCCAGCTGCC TCCACTAATC TACAAAAAGC ACTTTCTAAA 
GCACTGGAAA GCCGGGATGA GTTGAAAACC AAGTGGGGTG TTTCTGACTT CATCAAGGCC 
TTTATTAAAT TCCACGGTCA TGTATACCTG AGTAAGAGTT TGGAAAAGCT GAGCCCTCTT 
CGAGAGAAGT TGGAAGAACA GTTTAAGAGA CTGCTGTTCC AAAAGGCTTT CAACTCTCAA 
CAGTTAGTTC ATGTCACTGT CATTAACCTG TTTCAACTTC ATCATCTTCG TGACTTTAGC 
AATGAAACAG AGCAACACAG TTACAGCCAA GATGAACAGC TGTGTTGGAC ACAGTTGCTG 
GCCCTCTTTA TGTCTTTTCT CGGCATCCTG TGCAAATGTC CTCTCCAGAA TGATTCTCAG 
GAGTCCAACA ATGCCTACCC CCTTCCTGCA GTCAAGGTCT CCATGGACTG GCTAAGACTC 
CGACCTAGAG TCTTTCAAGA AGCAGTGGTG GATGAAAGAC AGTACATTTG GCCCTGGCTA 
ATTTCTCTTC TAAATAGTTT CCATCCCCGT GAAGATGATC TCTCAAATAC TAATGCCACA 
CCACTTCCAG AGGAGTTTGA ATTACAAGGA TTCTTGGCTT TAAGACCTTC TTTCAGGAAC 
TTGGATTTTT CCAAAGGCCA TCAGGGTATT ACAGGAGACA AAGAGGGTCA ACAACGACGA 
ATACGACAGC AGCGTTTGAT CTCCATAGGG AAATGGATTG CTGATAACCA GCCACGGCTG 
ATTCAGTGTG AAAATGAGGT AGGGAAATTG TTATTTATCA CAGAAATCCC AGAATTAATA 
CTAGAAGACC CCAGTGAAGC CAAAGAGAAC CTCATCCTAC AAGAAACATC TGTGGTAGAG 
TCACTGGCTA CGGATGGGAG CCCAGGACTG AAATCAGTGC TGTCTACAGG CCGAAATCCA 
AGCAACAGCT GTGACTCAGG AGAGAAACCA GTGGTCACCT TTAAAGAGAA CATTAAGCCA 
CGAGAAGTGA ACCAAGGAAG AAGCTTTCCT CCCAAAGAGG TGAGAAGGGA CTGTAGCAAA 
GGCGTAACTG TAACTCAGGA GGACGGACAG AAGGACAGCA GCAAGAGGAG AGCTGAGACC 
AAGAGATGCA CCTTAGGAAA GTTGCAGGAA ACAGGAAAGC AGAGTGTGGC AGTGCAGGTA 
AAATCCCAGA CAGAACTAAG AAAGACTCCA GTGTCTGAAG CCAGGAAAAC GCCTGTCACT 
CAAACTCCAA GTCAAACGAG TAATTCTCAG TTCATCCCCA TCCATCACCC TGGAGCCTTC 
CCTCCTCTTC CCAGCCGACC AGGATTCCCG CCCCCAACAT ATGTTATCCC CCCTCCTGTG 
GCATTTTCTA TGGGCTCAGG TTACACCTTC CCAGCTGGTG TTTCTGTCCC AGGAACCTTT 
CTTCAGTCTA CAGCTCACTC TCCAGCAGGA AACCAGGTGC AAGCTGGGAA ACAGTCCCAC 
ATTCCTTACA GCCAGCAACG GCCCTCTGGA CCAGGGCCAA TGAACCAGGG ACCTCAACAG 
TCACAGCCAC CTTCCCAGCC ACCCCTTACA TCTTTACCAG CTCAGCCAAC AGCACAGTCT 
ACAAGCCAAT TGCAGGTTCA AGCTCTAGCT CAGCAACAGC AATCCCCTAC AAAAGTCATA 
CCAGCTTTGG GGAAAAGCCC GCCTCACCAC TCTGGATTCC AGCAGTATCA ACAGGCAGAT 
GCCTCCAAAC AGCTGTGGAA TCCCCCTCAG GTTCAAAGCC CACTAGGGAA AATTATGCCT 
GTGAAACAGT CCTACTACCT TCAGACCCAA GACCCTATAA AACTGTTTGA GCCGTCATTG 
CAACCTCCTG TAATACAGCA GCAGCCTCTA GAGAAAAAAA TGAAGCCTTT CCCCATGGAG 
CCATATAACC ATAATCCCTC AGAAGTCAAG GTCCCAGAGT TCTACTGGGA TTCTTCCTAC 
AGCATGGCCG ATAACAGAGC AGTAATGGCT CAGCAACCAA ATATGGACCG CAGGAGCAAA 
CGGTCACCTG GAGTCTTCCG TCCAGAACAG GATCCTGTGC CCAGGATGCC ATTTGAGAAA 
TCCTTATTGG AGAAGCCTTC TGAGCTCATG TCACATTCAT CTTCTTTCCT GTCCCTCACG 
GGGTTCTCTG TCAATCAGGA AAGATATCCA AACAGCAGTA TGTTCAATGA AGTGTATGGA 
AAAAACCTGA CAACCAGCTC CAAGGCAGAA CTGAACCCCT CAGTCGCCTC CCAGGAAACA 
TCACTGTACT CCCTTTTTGA AGGGACCCCA TGGTCTCCAT CACTTCCTGC CAGTTCAGAT 
CATTCGACAC CAGCCAGCCA GTCTCCTCAT TCCTCTAACC CAAGCAGCCT GCCCAGTTCT 
CCTCCAACGC ATAACCATAA TTCTGCTCCA TTCTCTAATT TTGGACCCAT TGGGACTCCA 
GATAACAGGG ATAGGCGGCC TGCAGATAGG TGGAAAACTG ATAAACCAGC CATGGGTGGT 
TTTGGTGTTG ATTATCTCTC AGCAACGTCA TCATCTGAGA GCAGTTGGCA TCAGGCTAGC 
ACTCCGAGTG GCACCTGGAC AGGCCACGGC CCCTCCATGG AGGATTCCTC TGCTGTCCTC 
ATGGAAAGCC TAAAGTCTAT CTGGTCCAGT TCCATGATGC ATCCTGGGCC CTCCGCTCTG 
GAGCAGTTGT TAATGCAGCA GAAGCAGAAA CAGCAGCGGG GACAAGGTGC CATGAACCCT 
CCACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Smg7 ( XM_017319979.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017175468.1 Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant X5, mRNA.

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CloneID	OMu28141
Clone ID Related Accession (Same CDS sequence)	XM_030253292.1 , XM_017319979.1
Accession Version	XM_017319979.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3291bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein SMG7 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGACCG AAAACTTGAA ATCAGAGGAG CATCTGAAAT CAAGTAATAT TAGGCAGGCA 
GAAGTCCTGA AGGCTGAAAT GACAGATTCT AAGCTGGGTC CGGCTGAGGT CTGGACATCC 
AGGCAAGCTC TGCAGGACTT GTACCAGAAA ATGCTAGTTA CTGATCTGGA ATACGCTTTA 
GACAAGAAAG TAGAACAGGA TCTCTGGAAT CATGCCTTTA AGAATCAGAT CACAACACTG 
CAAGGCCAAG CCAAGAATCG AGCAAACCCA AATCGAAGTG AAGTCCAGGC AAACCTTTCT 
CTGTTCCTAG AGGCAGCTAG CGGCTTCTAT ACTCAGTTAT TACAGGAACT GTGTACGGTG 
TTTAATGTAG ATTTGCCATG CCGTGTGAAG TCTTCCCAGT TGGGAATTAT TAGCAATAAA 
CAGACGCACA GCAGCACCAT AGTGAAGCCA CAGTCTAGCT CCTGCTCCTA CATTTGCCAA 
CACTGCCTCG TCCACCTTGG AGACATTGCT CGATACAGAA ATCAGACTAG CCAGGCAGAG 
TCCTACTATA GGCATGCAGC TCAGCTTGTC CCCTCCAATG GTCAGCCTTA CAATCAGTTG 
GCTATCTTAG CTTCTTCCAA AGGAGACCAC CTGACCACAA TTTTCTACTA CTGCAGAAGC 
ATTGCTGTGA AATTCCCTTT CCCAGCTGCC TCCACTAATC TACAAAAAGC ACTTTCTAAA 
GCACTGGAAA GCCGGGATGA GTTGAAAACC AAGTGGGGTG TTTCTGACTT CATCAAGGCC 
TTTATTAAAT TCCACGGTCA TGTATACCTG AGTAAGAGTT TGGAAAAGCT GAGCCCTCTT 
CGAGAGAAGT TGGAAGAACA GTTTAAGAGA CTGCTGTTCC AAAAGGCTTT CAACTCTCAA 
CAGTTAGTTC ATGTCACTGT CATTAACCTG TTTCAACTTC ATCATCTTCG TGACTTTAGC 
AATGAAACAG AGCAACACAG TTACAGCCAA GATGAACAGC TGTGTTGGAC ACAGTTGCTG 
GCCCTCTTTA TGTCTTTTCT CGGCATCCTG TGCAAATGTC CTCTCCAGAA TGATTCTCAG 
GAGTCCAACA ATGCCTACCC CCTTCCTGCA GTCAAGGTCT CCATGGACTG GCTAAGACTC 
CGACCTAGAG TCTTTCAAGA AGCAGTGGTG GATGAAAGAC AGTACATTTG GCCCTGGCTA 
ATTTCTCTTC TAAATAGTTT CCATCCCCGT GAAGATGATC TCTCAAATAC TAATGCCACA 
CCACTTCCAG AGGAGTTTGA ATTACAAGGA TTCTTGGCTT TAAGACCTTC TTTCAGGAAC 
TTGGATTTTT CCAAAGGCCA TCAGGGTATT ACAGGAGACA AAGAGGGTCA ACAACGACGA 
ATACGACAGC AGCGTTTGAT CTCCATAGGG AAATGGATTG CTGATAACCA GCCACGGCTG 
ATTCAGTGTG AAAATGAGGT AGGGAAATTG TTATTTATCA CAGAAATCCC AGAATTAATA 
CTAGAAGACC CCAGTGAAGC CAAAGAGAAC CTCATCCTAC AAGAAACATC TGTGGTAGAG 
TCACTGGCTA CGGATGGGAG CCCAGGACTG AAATCAGTGC TGTCTACAGG CCGAAATCCA 
AGCAACAGCT GTGACTCAGG AGAGAAACCA GTGGTCACCT TTAAAGAGAA CATTAAGCCA 
CGAGAAGTGA ACCAAGGAAG AAGCTTTCCT CCCAAAGAGG TAAAATCCCA GACAGAACTA 
AGAAAGACTC CAGTGTCTGA AGCCAGGAAA ACGCCTGTCA CTCAAACTCC AAGTCAAACG 
AGTAATTCTC AGTTCATCCC CATCCATCAC CCTGGAGCCT TCCCTCCTCT TCCCAGCCGA 
CCAGGATTCC CGCCCCCAAC ATATGTTATC CCCCCTCCTG TGGCATTTTC TATGGGCTCA 
GGTTACACCT TCCCAGCTGG TGTTTCTGTC CCAGGAACCT TTCTTCAGTC TACAGCTCAC 
TCTCCAGCAG GAAACCAGGT GCAAGCTGGG AAACAGTCCC ACATTCCTTA CAGCCAGCAA 
CGGCCCTCTG GACCAGGGCC AATGAACCAG GGACCTCAAC AGTCACAGCC ACCTTCCCAG 
CCACCCCTTA CATCTTTACC AGCTCAGCCA ACAGCACAGT CTACAAGCCA ATTGCAGGTT 
CAAGCTCTAG CTCAGCAACA GCAATCCCCT ACAAAAGTCA TACCAGCTTT GGGGAAAAGC 
CCGCCTCACC ACTCTGGATT CCAGCAGTAT CAACAGGCAG ATGCCTCCAA ACAGCTGTGG 
AATCCCCCTC AGGTTCAAAG CCCACTAGGG AAAATTATGC CTGTGAAACA GTCCTACTAC 
CTTCAGACCC AAGACCCTAT AAAACTGTTT GAGCCGTCAT TGCAACCTCC TGTAATACAG 
CAGCAGCCTC TAGAGAAAAA AATGAAGCCT TTCCCCATGG AGCCATATAA CCATAATCCC 
TCAGAAGTCA AGGTCCCAGA GTTCTACTGG GATTCTTCCT ACAGCATGGC CGATAACAGA 
GCAGTAATGG CTCAGCAACC AAATATGGAC CGCAGGAGCA AACGGTCACC TGGAGTCTTC 
CGTCCAGAAC AGGATCCTGT GCCCAGGATG CCATTTGAGA AATCCTTATT GGAGAAGCCT 
TCTGAGCTCA TGTCACATTC ATCTTCTTTC CTGTCCCTCA CGGGGTTCTC TGTCAATCAG 
GAAAGATATC CAAACAGCAG TATGTTCAAT GAAGTGTATG GAAAAAACCT GACAACCAGC 
TCCAAGGCAG AACTGAACCC CTCAGTCGCC TCCCAGGAAA CATCACTGTA CTCCCTTTTT 
GAAGGGACCC CATGGTCTCC ATCACTTCCT GCCAGTTCAG ATCATTCGAC ACCAGCCAGC 
CAGTCTCCTC ATTCCTCTAA CCCAAGCAGC CTGCCCAGTT CTCCTCCAAC GCATAACCAT 
AATTCTGCTC CATTCTCTAA TTTTGGACCC ATTGGGACTC CAGATAACAG GGATAGGCGG 
CCTGCAGATA GGTGGAAAAC TGATAAACCA GCCATGGGTG GTTTTGGTGT TGATTATCTC 
TCAGCAACGT CATCATCTGA GAGCAGTTGG CATCAGGCTA GCACTCCGAG TGGCACCTGG 
ACAGGCCACG GCCCCTCCAT GGAGGATTCC TCTGCTGTCC TCATGGAAAG CCTAAAGTCT 
ATCTGGTCCA GTTCCATGAT GCATCCTGGG CCCTCCGCTC TGGAGCAGTT GTTAATGCAG 
CAGAAGCAGA AACAGCAGCG GGGACAAGGT GCCATGAACC CTCCACACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017175468.1
CDS	85..3375
Translation	MRTENLKSEEHLKSSNIRQAEVLKAEMTDSKLGPAEVWTSRQALQDLYQKMLVTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRANPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQTHSSTIVKPQSSSCSYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNLQKALSKALESRDELKTKWGVSDFIKAFIKFHGHVYLSKSLEKLSPLREKLEEQFKRLLFQKAFNSQQLVHVTVINLFQLHHLRDFSNETEQHSYSQDEQLCWTQLLALFMSFLGILCKCPLQNDSQESNNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPREDDLSNTNATPLPEEFELQGFLALRPSFRNLDFSKGHQGITGDKEGQQRRIRQQRLISIGKWIADNQPRLIQCENEVGKLLFITEIPELILEDPSEAKENLILQETSVVESLATDGSPGLKSVLSTGRNPSNSCDSGEKPVVTFKENIKPREVNQGRSFPPKEVKSQTELRKTPVSEARKTPVTQTPSQTSNSQFIPIHHPGAFPPLPSRPGFPPPTYVIPPPVAFSMGSGYTFPAGVSVPGTFLQSTAHSPAGNQVQAGKQSHIPYSQQRPSGPGPMNQGPQQSQPPSQPPLTSLPAQPTAQSTSQLQVQALAQQQQSPTKVIPALGKSPPHHSGFQQYQQADASKQLWNPPQVQSPLGKIMPVKQSYYLQTQDPIKLFEPSLQPPVIQQQPLEKKMKPFPMEPYNHNPSEVKVPEFYWDSSYSMADNRAVMAQQPNMDRRSKRSPGVFRPEQDPVPRMPFEKSLLEKPSELMSHSSSFLSLTGFSVNQERYPNSSMFNEVYGKNLTTSSKAELNPSVASQETSLYSLFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSAPFSNFGPIGTPDNRDRRPADRWKTDKPAMGGFGVDYLSATSSSESSWHQASTPSGTWTGHGPSMEDSSAVLMESLKSIWSSSMMHPGPSALEQLLMQQKQKQQRGQGAMNPPH

Target ORF information:

RefSeq Version	XM_017319979.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319979.1

ORF Insert Sequence:

ATGAGGACCG AAAACTTGAA ATCAGAGGAG CATCTGAAAT CAAGTAATAT TAGGCAGGCA 
GAAGTCCTGA AGGCTGAAAT GACAGATTCT AAGCTGGGTC CGGCTGAGGT CTGGACATCC 
AGGCAAGCTC TGCAGGACTT GTACCAGAAA ATGCTAGTTA CTGATCTGGA ATACGCTTTA 
GACAAGAAAG TAGAACAGGA TCTCTGGAAT CATGCCTTTA AGAATCAGAT CACAACACTG 
CAAGGCCAAG CCAAGAATCG AGCAAACCCA AATCGAAGTG AAGTCCAGGC AAACCTTTCT 
CTGTTCCTAG AGGCAGCTAG CGGCTTCTAT ACTCAGTTAT TACAGGAACT GTGTACGGTG 
TTTAATGTAG ATTTGCCATG CCGTGTGAAG TCTTCCCAGT TGGGAATTAT TAGCAATAAA 
CAGACGCACA GCAGCACCAT AGTGAAGCCA CAGTCTAGCT CCTGCTCCTA CATTTGCCAA 
CACTGCCTCG TCCACCTTGG AGACATTGCT CGATACAGAA ATCAGACTAG CCAGGCAGAG 
TCCTACTATA GGCATGCAGC TCAGCTTGTC CCCTCCAATG GTCAGCCTTA CAATCAGTTG 
GCTATCTTAG CTTCTTCCAA AGGAGACCAC CTGACCACAA TTTTCTACTA CTGCAGAAGC 
ATTGCTGTGA AATTCCCTTT CCCAGCTGCC TCCACTAATC TACAAAAAGC ACTTTCTAAA 
GCACTGGAAA GCCGGGATGA GTTGAAAACC AAGTGGGGTG TTTCTGACTT CATCAAGGCC 
TTTATTAAAT TCCACGGTCA TGTATACCTG AGTAAGAGTT TGGAAAAGCT GAGCCCTCTT 
CGAGAGAAGT TGGAAGAACA GTTTAAGAGA CTGCTGTTCC AAAAGGCTTT CAACTCTCAA 
CAGTTAGTTC ATGTCACTGT CATTAACCTG TTTCAACTTC ATCATCTTCG TGACTTTAGC 
AATGAAACAG AGCAACACAG TTACAGCCAA GATGAACAGC TGTGTTGGAC ACAGTTGCTG 
GCCCTCTTTA TGTCTTTTCT CGGCATCCTG TGCAAATGTC CTCTCCAGAA TGATTCTCAG 
GAGTCCAACA ATGCCTACCC CCTTCCTGCA GTCAAGGTCT CCATGGACTG GCTAAGACTC 
CGACCTAGAG TCTTTCAAGA AGCAGTGGTG GATGAAAGAC AGTACATTTG GCCCTGGCTA 
ATTTCTCTTC TAAATAGTTT CCATCCCCGT GAAGATGATC TCTCAAATAC TAATGCCACA 
CCACTTCCAG AGGAGTTTGA ATTACAAGGA TTCTTGGCTT TAAGACCTTC TTTCAGGAAC 
TTGGATTTTT CCAAAGGCCA TCAGGGTATT ACAGGAGACA AAGAGGGTCA ACAACGACGA 
ATACGACAGC AGCGTTTGAT CTCCATAGGG AAATGGATTG CTGATAACCA GCCACGGCTG 
ATTCAGTGTG AAAATGAGGT AGGGAAATTG TTATTTATCA CAGAAATCCC AGAATTAATA 
CTAGAAGACC CCAGTGAAGC CAAAGAGAAC CTCATCCTAC AAGAAACATC TGTGGTAGAG 
TCACTGGCTA CGGATGGGAG CCCAGGACTG AAATCAGTGC TGTCTACAGG CCGAAATCCA 
AGCAACAGCT GTGACTCAGG AGAGAAACCA GTGGTCACCT TTAAAGAGAA CATTAAGCCA 
CGAGAAGTGA ACCAAGGAAG AAGCTTTCCT CCCAAAGAGG TAAAATCCCA GACAGAACTA 
AGAAAGACTC CAGTGTCTGA AGCCAGGAAA ACGCCTGTCA CTCAAACTCC AAGTCAAACG 
AGTAATTCTC AGTTCATCCC CATCCATCAC CCTGGAGCCT TCCCTCCTCT TCCCAGCCGA 
CCAGGATTCC CGCCCCCAAC ATATGTTATC CCCCCTCCTG TGGCATTTTC TATGGGCTCA 
GGTTACACCT TCCCAGCTGG TGTTTCTGTC CCAGGAACCT TTCTTCAGTC TACAGCTCAC 
TCTCCAGCAG GAAACCAGGT GCAAGCTGGG AAACAGTCCC ACATTCCTTA CAGCCAGCAA 
CGGCCCTCTG GACCAGGGCC AATGAACCAG GGACCTCAAC AGTCACAGCC ACCTTCCCAG 
CCACCCCTTA CATCTTTACC AGCTCAGCCA ACAGCACAGT CTACAAGCCA ATTGCAGGTT 
CAAGCTCTAG CTCAGCAACA GCAATCCCCT ACAAAAGTCA TACCAGCTTT GGGGAAAAGC 
CCGCCTCACC ACTCTGGATT CCAGCAGTAT CAACAGGCAG ATGCCTCCAA ACAGCTGTGG 
AATCCCCCTC AGGTTCAAAG CCCACTAGGG AAAATTATGC CTGTGAAACA GTCCTACTAC 
CTTCAGACCC AAGACCCTAT AAAACTGTTT GAGCCGTCAT TGCAACCTCC TGTAATACAG 
CAGCAGCCTC TAGAGAAAAA AATGAAGCCT TTCCCCATGG AGCCATATAA CCATAATCCC 
TCAGAAGTCA AGGTCCCAGA GTTCTACTGG GATTCTTCCT ACAGCATGGC CGATAACAGA 
GCAGTAATGG CTCAGCAACC AAATATGGAC CGCAGGAGCA AACGGTCACC TGGAGTCTTC 
CGTCCAGAAC AGGATCCTGT GCCCAGGATG CCATTTGAGA AATCCTTATT GGAGAAGCCT 
TCTGAGCTCA TGTCACATTC ATCTTCTTTC CTGTCCCTCA CGGGGTTCTC TGTCAATCAG 
GAAAGATATC CAAACAGCAG TATGTTCAAT GAAGTGTATG GAAAAAACCT GACAACCAGC 
TCCAAGGCAG AACTGAACCC CTCAGTCGCC TCCCAGGAAA CATCACTGTA CTCCCTTTTT 
GAAGGGACCC CATGGTCTCC ATCACTTCCT GCCAGTTCAG ATCATTCGAC ACCAGCCAGC 
CAGTCTCCTC ATTCCTCTAA CCCAAGCAGC CTGCCCAGTT CTCCTCCAAC GCATAACCAT 
AATTCTGCTC CATTCTCTAA TTTTGGACCC ATTGGGACTC CAGATAACAG GGATAGGCGG 
CCTGCAGATA GGTGGAAAAC TGATAAACCA GCCATGGGTG GTTTTGGTGT TGATTATCTC 
TCAGCAACGT CATCATCTGA GAGCAGTTGG CATCAGGCTA GCACTCCGAG TGGCACCTGG 
ACAGGCCACG GCCCCTCCAT GGAGGATTCC TCTGCTGTCC TCATGGAAAG CCTAAAGTCT 
ATCTGGTCCA GTTCCATGAT GCATCCTGGG CCCTCCGCTC TGGAGCAGTT GTTAATGCAG 
CAGAAGCAGA AACAGCAGCG GGGACAAGGT GCCATGAACC CTCCACACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	Smg7
Entrez Gene ID	226517
Full Name	Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans)
Synonyms	9430023P16Rik,mKIAA0250
General protein information	Preferred Names Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) Names protein SMG7 EST1-like protein C
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1\|1 G3

	SMG7	XP_002802068.1
Mus musculus (house mouse)	Smg7	NP_001005507.1
Danio rerio (zebrafish)	smg7	NP_001038548.2
Bos taurus (cattle)	SMG7	NP_001180134.2
Xenopus tropicalis (tropical clawed frog)	smg7	NP_989258.1
Homo sapiens (human)	SMG7	NP_775179.1
Pan troglodytes (chimpanzee)	SMG7	XP_514048.3
Gallus gallus (chicken)	SMG7	XP_004943329.1
Canis lupus familiaris (dog)	SMG7	XP_537157.4
Rattus norvegicus (Norway rat)	Smg7	NP_001178478.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

Smg7 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

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