GAK gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_010806359.2	XP_010804661.1	cyclin-G-associated kinase isoform X3
XM_024993151.1	XP_024848919.1	cyclin-G-associated kinase isoform X1
XM_005208283.2	XP_005208340.2	cyclin-G-associated kinase isoform X3
XM_010806358.2	XP_010804660.1	cyclin-G-associated kinase isoform X1
XM_015471751.1	XP_015327237.1	cyclin-G-associated kinase isoform X2
XM_010806358.3	XP_010804660.2	cyclin-G-associated kinase isoform X2
XM_024993152.1	XP_024848920.1	cyclin-G-associated kinase isoform X3
NM_001046084.2	NP_001039549.1	cyclin-G-associated kinase
XM_024993153.1	XP_024848921.1	cyclin-G-associated kinase isoform X3
XM_005208283.1	XP_005208340.1	cyclin-G-associated kinase isoform X2
XM_010806359.3	XP_010804661.2	cyclin-G-associated kinase isoform X4

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa235256	XM_024993151.1 Latest version!	Bos taurus cyclin G associated kinase (GAK), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa235257	XM_010806358.3 Latest version!	Bos taurus cyclin G associated kinase (GAK), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa235258	XM_024993153.1 Latest version!	Bos taurus cyclin G associated kinase (GAK), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa235258	XM_024993152.1 Latest version!	Bos taurus cyclin G associated kinase (GAK), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa235258	XM_005208283.2 Latest version!	Bos taurus cyclin G associated kinase (GAK), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa235259	XM_010806359.3 Latest version!	Bos taurus cyclin G associated kinase (GAK), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa40735	NM_001046084.2 Latest version!	Bos taurus cyclin G associated kinase (GAK), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa20662	XM_010806358.2 Latest version!	Bos taurus cyclin G associated kinase (GAK), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa20663	XM_005208283.1 Latest version!	Bos taurus cyclin G associated kinase (GAK), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa20663	XM_015471751.1 Latest version!	Bos taurus cyclin G associated kinase (GAK), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa47972	XM_010806359.2 Latest version!	Bos taurus cyclin G associated kinase (GAK), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00

GAK ( XM_024993151.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024848919.1 Bos taurus cyclin G associated kinase (GAK), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa235256
Clone ID Related Accession (Same CDS sequence)	XM_024993151.1
Accession Version	XM_024993151.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3663bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	cyclin-G-associated kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCTGC TGCAGTCCGC GCTCGACTTC CTGGCGGGCC CGGGCTCCCT GGGGGGCGCG 
GCCGGCCGCG ACCAGACGGA CTTCGTGGGG CAGACGGTGG AGATGGGCGA GCTGCGGCTG 
CGGGTGCGCC GGGTCCTGGC TGAAGGAGGG TTTGCATTTG TGTATGAAGC TCAAGATCTG 
GGAAGTGGCA GAGAGTATGC ATTGAAGAGA CTGCTGTCCA ACGAAGAGGA GAAGAACAGA 
GCCATCATTC AGGAAGTGTG TTTCATGAAA AAACTTTCAG GCCACCCAAA CATTGTCCAG 
TTTTGTTCTG CAGCATCGAT AGGAAAAGAG GAGTCGGACA CTGGGCAGGC GGAGTTCCTG 
CTGCTCATGG AGCTCTGCAG AGGGCAGCTG GTGGAATTTT TAAAAAAAGT TGAATCGAAA 
GGTCCGCTCT CGTGCGACAC CGTCCTGAAG ATCTTCTACC AGACGTGCCG GGCGGTGCAG 
CACATGCACA GACAGAAGCC GCCCATCATC CACAGGGACC TCAAGGTTGA AAACTTACTG 
CTTAGTAACC AGGGGACCAT CAAGCTGTGT GACTTCGGCA GTGCCACGAC CATCTCACAC 
TACCCAGACT ACAGCTGGAG CGCCCAGCGC CGGGCCCTGG TGGAGGAGGA GATCACCAGG 
AACACGACCC CCATGTACAG GACGCCGGAA ATTGTGGACT TGTACTCAAA CTTTCCGATT 
GGCGAGAAGC AGGACATCTG GGCCCTGGGC TGCATCCTGT ACCTGCTCTG CTTCGGGCAG 
CACCCCTTTG AGGACGGAGC GAAGCTTCGC ATCGTCAACG GGAAGTACGC AATCCCTGCC 
GACGACACGC GGTACTCCGT GTTCCACAGC CTCATCCGTT CCATGCTCAA GGTCAACCCG 
GAGGAGCGGC TGTCCGTCAC CGAGCTGGTC AACCAGCTCC AGGAGATCGC GGCCGCCCGC 
AACGTGAACC CCAAGTCACC CATCACTGAG CTCCTGGAGC AGAATGGAGG CTATGGGCAC 
GCGGCCCCTC CCGGCTCCAC GGGCAGCAGT CTGGTGGTCA CTGAGTACGA GCAGCCCTAT 
GGCGGGTTCC TGGACATTCT GCGTGGGGGC ACCGAGCGCC TCTTCACCAA CATCAAGGAC 
ACCTCCTCCA AAGTCATCCA GTCCGTTGCC AACTATGCGA AGGGAGATCT GGATATTTCT 
TACATCACGT CTAGAATCGC AGTCATGTCG TTCCCGGCGG AGGGCGTGGA GTCGGCCATC 
AAGAACAACA TTGAGGATGT ACGGCTGTTC CTGGACTCGA AGCACCCGGG CCGCTACGCC 
GTCTACAACC TGTCCCCGCG GACCTACCGG CCCTCGAAGT TCCACAACCG GGTCTCCGAC 
TGCGGCTGGG TGGCCCGGCG GGCCCCGAGC CTCCGCAGCC TGTACAGCCT CTGCAGGAAC 
ATGCACGCCT GGCTGCGCCG CGACCCTGGC AACGTCTGCG TGGTGCACTG CATGGACGGG 
AGGGCGGTGT CGGCCGTGGC CGTGTGCTCC TTCTTGTGCT TCTGCCGGCT CTTCAGCACC 
GCGGAGGCCG CTGTGTACAT GTTCAGCATG AAGCGGTGCC CACCGGGCAT CTGGCCATCC 
CACAAGAGGT ACATTGAGTA CATGTGTGAC ATGGTGGCCG AGGAGCCCGT CACGCCCCAC 
AGCAAGCCCA TCCTGGTGAA GGCCGTGGTC ATGACGCCTG TGCCGCTCTT CAGCAAGCAG 
AGGAGCGGCT GCAGGCCCTT CTGCGAGGTC TACGTGGGGG ATGAGCGCGT GACCACCACG 
TCCCAGGAAT ACGACAAGAT GAGAGACTTT AAAATTGAGG ATGGCAAAGC GGTCATTCCC 
CTGGGCATAA CAGTCCAGGG GGACGTGCTC ATCATCATCT ATCATGCGAG GTCCACCCTG 
GGAGGAAGGC TGCAGGCCAA GATGGCGTCC ATGAAGATGT TTCAGATCCA GTTTCACACA 
GGGTTCGTGC CTCGGAACGC AGCTACTGTG AAGTTTGCCA AGTATGATCT GGATGCTTGC 
GACATCCAGG AGAAGTACCC CGACTTGTTC CAAGTGAGCC TGGAGGTGGA GGTGGAGCCC 
AGAGACAGGC CCGGCCACGA GGCCCCGCCC TGGGAGGGTG CAGGCATGCG AGGGCTGAAC 
CCCAAGATCC TCTTCTCCAG CCGGGAGGAG CAGCAAGATG TCCTGTCCAA GTTTGAGGAT 
CGGGACTCCA GTGTGGGCCC GGAGGGTCAC CTGGCCGAGG AGGGTCCCTC TGCTGTGGCC 
GAGGACGGCT GTGTGAGTGA GCCATCGGAC GAGGAAGCCC CGACACCCTC TGCGGAGAGC 
GGGGACACAG GCGATGAGGA CGTGCCCGCC CCCGCCGCCC CGCAGGACGA CAGCGTGGAC 
CTTCTGGGGC TGCACGCGGA GGCGGGGCCT GTGCCCGCCC CCCAGGCCCC CGGCGGCCCC 
CCCAGCAATG CAGACCTGCT CGGCTGCCTG CTCGGGGCCC CGGGCCCCGC TCCGGAGGGT 
GCTGTGGGGG GCCTGCTCGA CGGGGATGCG GCCCTGGTCT TCAGCAGCCC AGCCCCCGCG 
CCTTGCACCC AGACAACTGC CCCGGCCGCT GACCCGTTCG GCCCGCTCCT GCTGCCGCCC 
GACGTGGACA CCCCTCCTAG CTCGCAGCCT GACCTCTTCG GGGAATTCCT CAACTCAGAC 
GCCCCGGCCG CCCAGCCCGC GCCCTTCCCC TCCACCCACA GCGCTCCGCC GCCAGCCTGC 
AGCACCGACT TCCTGCATCT TGGGGACCTG CCCGCCGAGC CCAGCAAGAT GACAGCCTCT 
GCCAGCCACC CCGACCTGCT GGGAGCCTGG GATGCCTGGG CTGAGGCGCC GGCCCCCGCG 
CTGGCTGCAG ACGGCACCTT CTTCTCTCCT GGAGACCAGC CAGCCCCTTC TGGCCTTCCG 
GCTGGCCAGA CCAAGTCTCA GAGCCAGGAC CCGTTTGCAG ACCTTGGCGA CCTCAGTGCC 
GGCCTCCAAG GCTCGCCTGC CGCAGCTGCT CCTGGGGGCT TTGGTCCCAA AGCAGCGCCC 
CCTCCCAAGG CCAGCAGCTC CTGGCAGACG AGTCGGCCCC CAGCCCAGGG CACCCCGTGG 
CCCCCCCAGG CCAAGCCGCC CCCCAAAGCC AGTGTACCGC CAAGGCCTAA CTACACCTCC 
AGCTTCAGTG TGATCGGTAG CCGGGAGGAG CGGGGGGTCC GCGCCCCCAG CTTTGGTCCA 
AAGCCGAGGG TGTCAGAGAA CGACTTTGAA GACCTGCTCT CCAACCAGGG CTTCTCCTCG 
CGGGCTGACA GGAAGGGGCC GCGGACCATC GCTGAGATGA GGAGGCAGGA CCAGGCCCGG 
GACTTGGATC CGCTGAAGCT GAAGCTTCTG GAGTGGACGG AGGGCAAGGA GAGGAACATC 
CGCGCCCTGC TGTCCACGCT GCACACGGCG CTGTGGGACG GCGAGAGCCG CTGGACGCCG 
GTGGGCATGG CCGACCTGGT GACGCCCGCA CAAGTGAAGA AGCACTACCG CCGTGCCGTG 
CTGGTGGTCC ACCCCGACAA GGCCCGGGGG CAGCCCTACG AGCAGTATGC CCGGATGATC 
TTCATGGAGC TCAACGATGC GTGGGCTGAG TTTGAGAGCC AGGGCTCCCG GCCACTCTTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024848919.1
CDS	114..3776
Translation	MSLLQSALDFLAGPGSLGGAAGRDQTDFVGQTVEMGELRLRVRRVLAEGGFAFVYEAQDLGSGREYALKRLLSNEEEKNRAIIQEVCFMKKLSGHPNIVQFCSAASIGKEESDTGQAEFLLLMELCRGQLVEFLKKVESKGPLSCDTVLKIFYQTCRAVQHMHRQKPPIIHRDLKVENLLLSNQGTIKLCDFGSATTISHYPDYSWSAQRRALVEEEITRNTTPMYRTPEIVDLYSNFPIGEKQDIWALGCILYLLCFGQHPFEDGAKLRIVNGKYAIPADDTRYSVFHSLIRSMLKVNPEERLSVTELVNQLQEIAAARNVNPKSPITELLEQNGGYGHAAPPGSTGSSLVVTEYEQPYGGFLDILRGGTERLFTNIKDTSSKVIQSVANYAKGDLDISYITSRIAVMSFPAEGVESAIKNNIEDVRLFLDSKHPGRYAVYNLSPRTYRPSKFHNRVSDCGWVARRAPSLRSLYSLCRNMHAWLRRDPGNVCVVHCMDGRAVSAVAVCSFLCFCRLFSTAEAAVYMFSMKRCPPGIWPSHKRYIEYMCDMVAEEPVTPHSKPILVKAVVMTPVPLFSKQRSGCRPFCEVYVGDERVTTTSQEYDKMRDFKIEDGKAVIPLGITVQGDVLIIIYHARSTLGGRLQAKMASMKMFQIQFHTGFVPRNAATVKFAKYDLDACDIQEKYPDLFQVSLEVEVEPRDRPGHEAPPWEGAGMRGLNPKILFSSREEQQDVLSKFEDRDSSVGPEGHLAEEGPSAVAEDGCVSEPSDEEAPTPSAESGDTGDEDVPAPAAPQDDSVDLLGLHAEAGPVPAPQAPGGPPSNADLLGCLLGAPGPAPEGAVGGLLDGDAALVFSSPAPAPCTQTTAPAADPFGPLLLPPDVDTPPSSQPDLFGEFLNSDAPAAQPAPFPSTHSAPPPACSTDFLHLGDLPAEPSKMTASASHPDLLGAWDAWAEAPAPALAADGTFFSPGDQPAPSGLPAGQTKSQSQDPFADLGDLSAGLQGSPAAAAPGGFGPKAAPPPKASSSWQTSRPPAQGTPWPPQAKPPPKASVPPRPNYTSSFSVIGSREERGVRAPSFGPKPRVSENDFEDLLSNQGFSSRADRKGPRTIAEMRRQDQARDLDPLKLKLLEWTEGKERNIRALLSTLHTALWDGESRWTPVGMADLVTPAQVKKHYRRAVLVVHPDKARGQPYEQYARMIFMELNDAWAEFESQGSRPLF

Target ORF information:

RefSeq Version	XM_024993151.1
Organism	Bos taurus(cattle)
Definition	Bos taurus cyclin G associated kinase (GAK), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024993151.1

ORF Insert Sequence:

ATGTCGCTGC TGCAGTCCGC GCTCGACTTC CTGGCGGGCC CGGGCTCCCT GGGGGGCGCG 
GCCGGCCGCG ACCAGACGGA CTTCGTGGGG CAGACGGTGG AGATGGGCGA GCTGCGGCTG 
CGGGTGCGCC GGGTCCTGGC TGAAGGAGGG TTTGCATTTG TGTATGAAGC TCAAGATCTG 
GGAAGTGGCA GAGAGTATGC ATTGAAGAGA CTGCTGTCCA ACGAAGAGGA GAAGAACAGA 
GCCATCATTC AGGAAGTGTG TTTCATGAAA AAACTTTCAG GCCACCCAAA CATTGTCCAG 
TTTTGTTCTG CAGCATCGAT AGGAAAAGAG GAGTCGGACA CTGGGCAGGC GGAGTTCCTG 
CTGCTCATGG AGCTCTGCAG AGGGCAGCTG GTGGAATTTT TAAAAAAAGT TGAATCGAAA 
GGTCCGCTCT CGTGCGACAC CGTCCTGAAG ATCTTCTACC AGACGTGCCG GGCGGTGCAG 
CACATGCACA GACAGAAGCC GCCCATCATC CACAGGGACC TCAAGGTTGA AAACTTACTG 
CTTAGTAACC AGGGGACCAT CAAGCTGTGT GACTTCGGCA GTGCCACGAC CATCTCACAC 
TACCCAGACT ACAGCTGGAG CGCCCAGCGC CGGGCCCTGG TGGAGGAGGA GATCACCAGG 
AACACGACCC CCATGTACAG GACGCCGGAA ATTGTGGACT TGTACTCAAA CTTTCCGATT 
GGCGAGAAGC AGGACATCTG GGCCCTGGGC TGCATCCTGT ACCTGCTCTG CTTCGGGCAG 
CACCCCTTTG AGGACGGAGC GAAGCTTCGC ATCGTCAACG GGAAGTACGC AATCCCTGCC 
GACGACACGC GGTACTCCGT GTTCCACAGC CTCATCCGTT CCATGCTCAA GGTCAACCCG 
GAGGAGCGGC TGTCCGTCAC CGAGCTGGTC AACCAGCTCC AGGAGATCGC GGCCGCCCGC 
AACGTGAACC CCAAGTCACC CATCACTGAG CTCCTGGAGC AGAATGGAGG CTATGGGCAC 
GCGGCCCCTC CCGGCTCCAC GGGCAGCAGT CTGGTGGTCA CTGAGTACGA GCAGCCCTAT 
GGCGGGTTCC TGGACATTCT GCGTGGGGGC ACCGAGCGCC TCTTCACCAA CATCAAGGAC 
ACCTCCTCCA AAGTCATCCA GTCCGTTGCC AACTATGCGA AGGGAGATCT GGATATTTCT 
TACATCACGT CTAGAATCGC AGTCATGTCG TTCCCGGCGG AGGGCGTGGA GTCGGCCATC 
AAGAACAACA TTGAGGATGT ACGGCTGTTC CTGGACTCGA AGCACCCGGG CCGCTACGCC 
GTCTACAACC TGTCCCCGCG GACCTACCGG CCCTCGAAGT TCCACAACCG GGTCTCCGAC 
TGCGGCTGGG TGGCCCGGCG GGCCCCGAGC CTCCGCAGCC TGTACAGCCT CTGCAGGAAC 
ATGCACGCCT GGCTGCGCCG CGACCCTGGC AACGTCTGCG TGGTGCACTG CATGGACGGG 
AGGGCGGTGT CGGCCGTGGC CGTGTGCTCC TTCTTGTGCT TCTGCCGGCT CTTCAGCACC 
GCGGAGGCCG CTGTGTACAT GTTCAGCATG AAGCGGTGCC CACCGGGCAT CTGGCCATCC 
CACAAGAGGT ACATTGAGTA CATGTGTGAC ATGGTGGCCG AGGAGCCCGT CACGCCCCAC 
AGCAAGCCCA TCCTGGTGAA GGCCGTGGTC ATGACGCCTG TGCCGCTCTT CAGCAAGCAG 
AGGAGCGGCT GCAGGCCCTT CTGCGAGGTC TACGTGGGGG ATGAGCGCGT GACCACCACG 
TCCCAGGAAT ACGACAAGAT GAGAGACTTT AAAATTGAGG ATGGCAAAGC GGTCATTCCC 
CTGGGCATAA CAGTCCAGGG GGACGTGCTC ATCATCATCT ATCATGCGAG GTCCACCCTG 
GGAGGAAGGC TGCAGGCCAA GATGGCGTCC ATGAAGATGT TTCAGATCCA GTTTCACACA 
GGGTTCGTGC CTCGGAACGC AGCTACTGTG AAGTTTGCCA AGTATGATCT GGATGCTTGC 
GACATCCAGG AGAAGTACCC CGACTTGTTC CAAGTGAGCC TGGAGGTGGA GGTGGAGCCC 
AGAGACAGGC CCGGCCACGA GGCCCCGCCC TGGGAGGGTG CAGGCATGCG AGGGCTGAAC 
CCCAAGATCC TCTTCTCCAG CCGGGAGGAG CAGCAAGATG TCCTGTCCAA GTTTGAGGAT 
CGGGACTCCA GTGTGGGCCC GGAGGGTCAC CTGGCCGAGG AGGGTCCCTC TGCTGTGGCC 
GAGGACGGCT GTGTGAGTGA GCCATCGGAC GAGGAAGCCC CGACACCCTC TGCGGAGAGC 
GGGGACACAG GCGATGAGGA CGTGCCCGCC CCCGCCGCCC CGCAGGACGA CAGCGTGGAC 
CTTCTGGGGC TGCACGCGGA GGCGGGGCCT GTGCCCGCCC CCCAGGCCCC CGGCGGCCCC 
CCCAGCAATG CAGACCTGCT CGGCTGCCTG CTCGGGGCCC CGGGCCCCGC TCCGGAGGGT 
GCTGTGGGGG GCCTGCTCGA CGGGGATGCG GCCCTGGTCT TCAGCAGCCC AGCCCCCGCG 
CCTTGCACCC AGACAACTGC CCCGGCCGCT GACCCGTTCG GCCCGCTCCT GCTGCCGCCC 
GACGTGGACA CCCCTCCTAG CTCGCAGCCT GACCTCTTCG GGGAATTCCT CAACTCAGAC 
GCCCCGGCCG CCCAGCCCGC GCCCTTCCCC TCCACCCACA GCGCTCCGCC GCCAGCCTGC 
AGCACCGACT TCCTGCATCT TGGGGACCTG CCCGCCGAGC CCAGCAAGAT GACAGCCTCT 
GCCAGCCACC CCGACCTGCT GGGAGCCTGG GATGCCTGGG CTGAGGCGCC GGCCCCCGCG 
CTGGCTGCAG ACGGCACCTT CTTCTCTCCT GGAGACCAGC CAGCCCCTTC TGGCCTTCCG 
GCTGGCCAGA CCAAGTCTCA GAGCCAGGAC CCGTTTGCAG ACCTTGGCGA CCTCAGTGCC 
GGCCTCCAAG GCTCGCCTGC CGCAGCTGCT CCTGGGGGCT TTGGTCCCAA AGCAGCGCCC 
CCTCCCAAGG CCAGCAGCTC CTGGCAGACG AGTCGGCCCC CAGCCCAGGG CACCCCGTGG 
CCCCCCCAGG CCAAGCCGCC CCCCAAAGCC AGTGTACCGC CAAGGCCTAA CTACACCTCC 
AGCTTCAGTG TGATCGGTAG CCGGGAGGAG CGGGGGGTCC GCGCCCCCAG CTTTGGTCCA 
AAGCCGAGGG TGTCAGAGAA CGACTTTGAA GACCTGCTCT CCAACCAGGG CTTCTCCTCG 
CGGGCTGACA GGAAGGGGCC GCGGACCATC GCTGAGATGA GGAGGCAGGA CCAGGCCCGG 
GACTTGGATC CGCTGAAGCT GAAGCTTCTG GAGTGGACGG AGGGCAAGGA GAGGAACATC 
CGCGCCCTGC TGTCCACGCT GCACACGGCG CTGTGGGACG GCGAGAGCCG CTGGACGCCG 
GTGGGCATGG CCGACCTGGT GACGCCCGCA CAAGTGAAGA AGCACTACCG CCGTGCCGTG 
CTGGTGGTCC ACCCCGACAA GGCCCGGGGG CAGCCCTACG AGCAGTATGC CCGGATGATC 
TTCATGGAGC TCAACGATGC GTGGGCTGAG TTTGAGAGCC AGGGCTCCCG GCCACTCTTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAK ( XM_010806358.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010804660.2 Bos taurus cyclin G associated kinase (GAK), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa235257
Clone ID Related Accession (Same CDS sequence)	XM_010806358.3
Accession Version	XM_010806358.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3543bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	cyclin-G-associated kinase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010806358.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAAAC TTTCAGGCCA CCCAAACATT GTCCAGTTTT GTTCTGCAGC ATCGATAGGA 
AAAGAGGAGT CGGACACTGG GCAGGCGGAG TTCCTGCTGC TCATGGAGCT CTGCAGAGGG 
CAGCTGGTGG AATTTTTAAA AAAAGTTGAA TCGAAAGGTC CGCTCTCGTG CGACACCGTC 
CTGAAGATCT TCTACCAGAC GTGCCGGGCG GTGCAGCACA TGCACAGACA GAAGCCGCCC 
ATCATCCACA GGGACCTCAA GGTTGAAAAC TTACTGCTTA GTAACCAGGG GACCATCAAG 
CTGTGTGACT TCGGCAGTGC CACGACCATC TCACACTACC CAGACTACAG CTGGAGCGCC 
CAGCGCCGGG CCCTGGTGGA GGAGGAGATC ACCAGGAACA CGACCCCCAT GTACAGGACG 
CCGGAAATTG TGGACTTGTA CTCAAACTTT CCGATTGGCG AGAAGCAGGA CATCTGGGCC 
CTGGGCTGCA TCCTGTACCT GCTCTGCTTC GGGCAGCACC CCTTTGAGGA CGGAGCGAAG 
CTTCGCATCG TCAACGGGAA GTACGCAATC CCTGCCGACG ACACGCGGTA CTCCGTGTTC 
CACAGCCTCA TCCGTTCCAT GCTCAAGGTC AACCCGGAGG AGCGGCTGTC CGTCACCGAG 
CTGGTCAACC AGCTCCAGGA GATCGCGGCC GCCCGCAACG TGAACCCCAA GTCACCCATC 
ACTGAGCTCC TGGAGCAGAA TGGAGGCTAT GGGCACGCGG CCCCTCCCGG CTCCACGGGC 
AGCAGTCTGG TGGTCACTGA GTACGAGCAG CCCTATGGCG GGTTCCTGGA CATTCTGCGT 
GGGGGCACCG AGCGCCTCTT CACCAACATC AAGGACACCT CCTCCAAAGT CATCCAGTCC 
GTTGCCAACT ATGCGAAGGG AGATCTGGAT ATTTCTTACA TCACGTCTAG AATCGCAGTC 
ATGTCGTTCC CGGCGGAGGG CGTGGAGTCG GCCATCAAGA ACAACATTGA GGATGTACGG 
CTGTTCCTGG ACTCGAAGCA CCCGGGCCGC TACGCCGTCT ACAACCTGTC CCCGCGGACC 
TACCGGCCCT CGAAGTTCCA CAACCGGGTC TCCGACTGCG GCTGGGTGGC CCGGCGGGCC 
CCGAGCCTCC GCAGCCTGTA CAGCCTCTGC AGGAACATGC ACGCCTGGCT GCGCCGCGAC 
CCTGGCAACG TCTGCGTGGT GCACTGCATG GACGGGAGGG CGGTGTCGGC CGTGGCCGTG 
TGCTCCTTCT TGTGCTTCTG CCGGCTCTTC AGCACCGCGG AGGCCGCTGT GTACATGTTC 
AGCATGAAGC GGTGCCCACC GGGCATCTGG CCATCCCACA AGAGGTACAT TGAGTACATG 
TGTGACATGG TGGCCGAGGA GCCCGTCACG CCCCACAGCA AGCCCATCCT GGTGAAGGCC 
GTGGTCATGA CGCCTGTGCC GCTCTTCAGC AAGCAGAGGA GCGGCTGCAG GCCCTTCTGC 
GAGGTCTACG TGGGGGATGA GCGCGTGACC ACCACGTCCC AGGAATACGA CAAGATGAGA 
GACTTTAAAA TTGAGGATGG CAAAGCGGTC ATTCCCCTGG GCATAACAGT CCAGGGGGAC 
GTGCTCATCA TCATCTATCA TGCGAGGTCC ACCCTGGGAG GAAGGCTGCA GGCCAAGATG 
GCGTCCATGA AGATGTTTCA GATCCAGTTT CACACAGGGT TCGTGCCTCG GAACGCAGCT 
ACTGTGAAGT TTGCCAAGTA TGATCTGGAT GCTTGCGACA TCCAGGAGAA GTACCCCGAC 
TTGTTCCAAG TGAGCCTGGA GGTGGAGGTG GAGCCCAGAG ACAGGCCCGG CCACGAGGCC 
CCGCCCTGGG AGGGTGCAGG CATGCGAGGG CTGAACCCCA AGATCCTCTT CTCCAGCCGG 
GAGGAGCAGC AAGATGTCCT GTCCAAGTTT GGGAAGCCAG AGCTCCCCAG GCAGCCTGGG 
TCCACCGCAC AGTACGACGC CGAGGCCGGG CCCGCGGATG CCGAGCCCCC CGAGTCAGAC 
TCGCCGCACA GCAGCGTGGA TGCTGACCAC TTCCTGCACA CGCTGGACTG GCGGGAGGAT 
CGGGACTCCA GTGTGGGCCC GGAGGGTCAC CTGGCCGAGG AGGGTCCCTC TGCTGTGGCC 
GAGGACGGCT GTGTGAGTGA GCCATCGGAC GAGGAAGCCC CGACACCCTC TGCGGAGAGC 
GGGGACACAG GCGATGAGGA CGTGCCCGCC CCCGCCGCCC CGCAGGACGA CAGCGTGGAC 
CTTCTGGGGC TGCACGCGGA GGCGGGGCCT GTGCCCGCCC CCCAGGCCCC CGGCGGCCCC 
CCCAGCAATG CAGACCTGCT CGGCTGCCTG CTCGGGGCCC CGGGCCCCGC TCCGGAGGGT 
GCTGTGGGGG GCCTGCTCGA CGGGGATGCG GCCCTGGTCT TCAGCAGCCC AGCCCCCGCG 
CCTTGCACCC AGACAACTGC CCCGGCCGCT GACCCGTTCG GCCCGCTCCT GCTGCCGCCC 
GACGTGGACA CCCCTCCTAG CTCGCAGCCT GACCTCTTCG GGGAATTCCT CAACTCAGAC 
GCCCCGGCCG CCCAGCCCGC GCCCTTCCCC TCCACCCACA GCGCTCCGCC GCCAGCCTGC 
AGCACCGACT TCCTGCATCT TGGGGACCTG CCCGCCGAGC CCAGCAAGAT GACAGCCTCT 
GCCAGCCACC CCGACCTGCT GGGAGCCTGG GATGCCTGGG CTGAGGCGCC GGCCCCCGCG 
CTGGCTGCAG ACGGCACCTT CTTCTCTCCT GGAGACCAGC CAGCCCCTTC TGGCCTTCCG 
GCTGGCCAGA CCAAGTCTCA GAGCCAGGAC CCGTTTGCAG ACCTTGGCGA CCTCAGTGCC 
GGCCTCCAAG GCTCGCCTGC CGCAGCTGCT CCTGGGGGCT TTGGTCCCAA AGCAGCGCCC 
CCTCCCAAGG CCAGCAGCTC CTGGCAGACG AGTCGGCCCC CAGCCCAGGG CACCCCGTGG 
CCCCCCCAGG CCAAGCCGCC CCCCAAAGCC AGTGTACCGC CAAGGCCTAA CTACACCTCC 
AGCTTCAGTG TGATCGGTAG CCGGGAGGAG CGGGGGGTCC GCGCCCCCAG CTTTGGTCCA 
AAGCCGAGGG TGTCAGAGAA CGACTTTGAA GACCTGCTCT CCAACCAGGG CTTCTCCTCG 
CGGGCTGACA GGAAGGGGCC GCGGACCATC GCTGAGATGA GGAGGCAGGA CCAGGCCCGG 
GACTTGGATC CGCTGAAGCT GAAGCTTCTG GAGTGGACGG AGGGCAAGGA GAGGAACATC 
CGCGCCCTGC TGTCCACGCT GCACACGGCG CTGTGGGACG GCGAGAGCCG CTGGACGCCG 
GTGGGCATGG CCGACCTGGT GACGCCCGCA CAAGTGAAGA AGCACTACCG CCGTGCCGTG 
CTGGTGGTCC ACCCCGACAA GGCCCGGGGG CAGCCCTACG AGCAGTATGC CCGGATGATC 
TTCATGGAGC TCAACGATGC GTGGGCTGAG TTTGAGAGCC AGGGCTCCCG GCCACTCTTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010804660.2
CDS	230..3772
Translation	MKKLSGHPNIVQFCSAASIGKEESDTGQAEFLLLMELCRGQLVEFLKKVESKGPLSCDTVLKIFYQTCRAVQHMHRQKPPIIHRDLKVENLLLSNQGTIKLCDFGSATTISHYPDYSWSAQRRALVEEEITRNTTPMYRTPEIVDLYSNFPIGEKQDIWALGCILYLLCFGQHPFEDGAKLRIVNGKYAIPADDTRYSVFHSLIRSMLKVNPEERLSVTELVNQLQEIAAARNVNPKSPITELLEQNGGYGHAAPPGSTGSSLVVTEYEQPYGGFLDILRGGTERLFTNIKDTSSKVIQSVANYAKGDLDISYITSRIAVMSFPAEGVESAIKNNIEDVRLFLDSKHPGRYAVYNLSPRTYRPSKFHNRVSDCGWVARRAPSLRSLYSLCRNMHAWLRRDPGNVCVVHCMDGRAVSAVAVCSFLCFCRLFSTAEAAVYMFSMKRCPPGIWPSHKRYIEYMCDMVAEEPVTPHSKPILVKAVVMTPVPLFSKQRSGCRPFCEVYVGDERVTTTSQEYDKMRDFKIEDGKAVIPLGITVQGDVLIIIYHARSTLGGRLQAKMASMKMFQIQFHTGFVPRNAATVKFAKYDLDACDIQEKYPDLFQVSLEVEVEPRDRPGHEAPPWEGAGMRGLNPKILFSSREEQQDVLSKFGKPELPRQPGSTAQYDAEAGPADAEPPESDSPHSSVDADHFLHTLDWREDRDSSVGPEGHLAEEGPSAVAEDGCVSEPSDEEAPTPSAESGDTGDEDVPAPAAPQDDSVDLLGLHAEAGPVPAPQAPGGPPSNADLLGCLLGAPGPAPEGAVGGLLDGDAALVFSSPAPAPCTQTTAPAADPFGPLLLPPDVDTPPSSQPDLFGEFLNSDAPAAQPAPFPSTHSAPPPACSTDFLHLGDLPAEPSKMTASASHPDLLGAWDAWAEAPAPALAADGTFFSPGDQPAPSGLPAGQTKSQSQDPFADLGDLSAGLQGSPAAAAPGGFGPKAAPPPKASSSWQTSRPPAQGTPWPPQAKPPPKASVPPRPNYTSSFSVIGSREERGVRAPSFGPKPRVSENDFEDLLSNQGFSSRADRKGPRTIAEMRRQDQARDLDPLKLKLLEWTEGKERNIRALLSTLHTALWDGESRWTPVGMADLVTPAQVKKHYRRAVLVVHPDKARGQPYEQYARMIFMELNDAWAEFESQGSRPLF

Target ORF information:

RefSeq Version	XM_010806358.3
Organism	Bos taurus(cattle)
Definition	Bos taurus cyclin G associated kinase (GAK), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010806358.3

ORF Insert Sequence:

ATGAAAAAAC TTTCAGGCCA CCCAAACATT GTCCAGTTTT GTTCTGCAGC ATCGATAGGA 
AAAGAGGAGT CGGACACTGG GCAGGCGGAG TTCCTGCTGC TCATGGAGCT CTGCAGAGGG 
CAGCTGGTGG AATTTTTAAA AAAAGTTGAA TCGAAAGGTC CGCTCTCGTG CGACACCGTC 
CTGAAGATCT TCTACCAGAC GTGCCGGGCG GTGCAGCACA TGCACAGACA GAAGCCGCCC 
ATCATCCACA GGGACCTCAA GGTTGAAAAC TTACTGCTTA GTAACCAGGG GACCATCAAG 
CTGTGTGACT TCGGCAGTGC CACGACCATC TCACACTACC CAGACTACAG CTGGAGCGCC 
CAGCGCCGGG CCCTGGTGGA GGAGGAGATC ACCAGGAACA CGACCCCCAT GTACAGGACG 
CCGGAAATTG TGGACTTGTA CTCAAACTTT CCGATTGGCG AGAAGCAGGA CATCTGGGCC 
CTGGGCTGCA TCCTGTACCT GCTCTGCTTC GGGCAGCACC CCTTTGAGGA CGGAGCGAAG 
CTTCGCATCG TCAACGGGAA GTACGCAATC CCTGCCGACG ACACGCGGTA CTCCGTGTTC 
CACAGCCTCA TCCGTTCCAT GCTCAAGGTC AACCCGGAGG AGCGGCTGTC CGTCACCGAG 
CTGGTCAACC AGCTCCAGGA GATCGCGGCC GCCCGCAACG TGAACCCCAA GTCACCCATC 
ACTGAGCTCC TGGAGCAGAA TGGAGGCTAT GGGCACGCGG CCCCTCCCGG CTCCACGGGC 
AGCAGTCTGG TGGTCACTGA GTACGAGCAG CCCTATGGCG GGTTCCTGGA CATTCTGCGT 
GGGGGCACCG AGCGCCTCTT CACCAACATC AAGGACACCT CCTCCAAAGT CATCCAGTCC 
GTTGCCAACT ATGCGAAGGG AGATCTGGAT ATTTCTTACA TCACGTCTAG AATCGCAGTC 
ATGTCGTTCC CGGCGGAGGG CGTGGAGTCG GCCATCAAGA ACAACATTGA GGATGTACGG 
CTGTTCCTGG ACTCGAAGCA CCCGGGCCGC TACGCCGTCT ACAACCTGTC CCCGCGGACC 
TACCGGCCCT CGAAGTTCCA CAACCGGGTC TCCGACTGCG GCTGGGTGGC CCGGCGGGCC 
CCGAGCCTCC GCAGCCTGTA CAGCCTCTGC AGGAACATGC ACGCCTGGCT GCGCCGCGAC 
CCTGGCAACG TCTGCGTGGT GCACTGCATG GACGGGAGGG CGGTGTCGGC CGTGGCCGTG 
TGCTCCTTCT TGTGCTTCTG CCGGCTCTTC AGCACCGCGG AGGCCGCTGT GTACATGTTC 
AGCATGAAGC GGTGCCCACC GGGCATCTGG CCATCCCACA AGAGGTACAT TGAGTACATG 
TGTGACATGG TGGCCGAGGA GCCCGTCACG CCCCACAGCA AGCCCATCCT GGTGAAGGCC 
GTGGTCATGA CGCCTGTGCC GCTCTTCAGC AAGCAGAGGA GCGGCTGCAG GCCCTTCTGC 
GAGGTCTACG TGGGGGATGA GCGCGTGACC ACCACGTCCC AGGAATACGA CAAGATGAGA 
GACTTTAAAA TTGAGGATGG CAAAGCGGTC ATTCCCCTGG GCATAACAGT CCAGGGGGAC 
GTGCTCATCA TCATCTATCA TGCGAGGTCC ACCCTGGGAG GAAGGCTGCA GGCCAAGATG 
GCGTCCATGA AGATGTTTCA GATCCAGTTT CACACAGGGT TCGTGCCTCG GAACGCAGCT 
ACTGTGAAGT TTGCCAAGTA TGATCTGGAT GCTTGCGACA TCCAGGAGAA GTACCCCGAC 
TTGTTCCAAG TGAGCCTGGA GGTGGAGGTG GAGCCCAGAG ACAGGCCCGG CCACGAGGCC 
CCGCCCTGGG AGGGTGCAGG CATGCGAGGG CTGAACCCCA AGATCCTCTT CTCCAGCCGG 
GAGGAGCAGC AAGATGTCCT GTCCAAGTTT GGGAAGCCAG AGCTCCCCAG GCAGCCTGGG 
TCCACCGCAC AGTACGACGC CGAGGCCGGG CCCGCGGATG CCGAGCCCCC CGAGTCAGAC 
TCGCCGCACA GCAGCGTGGA TGCTGACCAC TTCCTGCACA CGCTGGACTG GCGGGAGGAT 
CGGGACTCCA GTGTGGGCCC GGAGGGTCAC CTGGCCGAGG AGGGTCCCTC TGCTGTGGCC 
GAGGACGGCT GTGTGAGTGA GCCATCGGAC GAGGAAGCCC CGACACCCTC TGCGGAGAGC 
GGGGACACAG GCGATGAGGA CGTGCCCGCC CCCGCCGCCC CGCAGGACGA CAGCGTGGAC 
CTTCTGGGGC TGCACGCGGA GGCGGGGCCT GTGCCCGCCC CCCAGGCCCC CGGCGGCCCC 
CCCAGCAATG CAGACCTGCT CGGCTGCCTG CTCGGGGCCC CGGGCCCCGC TCCGGAGGGT 
GCTGTGGGGG GCCTGCTCGA CGGGGATGCG GCCCTGGTCT TCAGCAGCCC AGCCCCCGCG 
CCTTGCACCC AGACAACTGC CCCGGCCGCT GACCCGTTCG GCCCGCTCCT GCTGCCGCCC 
GACGTGGACA CCCCTCCTAG CTCGCAGCCT GACCTCTTCG GGGAATTCCT CAACTCAGAC 
GCCCCGGCCG CCCAGCCCGC GCCCTTCCCC TCCACCCACA GCGCTCCGCC GCCAGCCTGC 
AGCACCGACT TCCTGCATCT TGGGGACCTG CCCGCCGAGC CCAGCAAGAT GACAGCCTCT 
GCCAGCCACC CCGACCTGCT GGGAGCCTGG GATGCCTGGG CTGAGGCGCC GGCCCCCGCG 
CTGGCTGCAG ACGGCACCTT CTTCTCTCCT GGAGACCAGC CAGCCCCTTC TGGCCTTCCG 
GCTGGCCAGA CCAAGTCTCA GAGCCAGGAC CCGTTTGCAG ACCTTGGCGA CCTCAGTGCC 
GGCCTCCAAG GCTCGCCTGC CGCAGCTGCT CCTGGGGGCT TTGGTCCCAA AGCAGCGCCC 
CCTCCCAAGG CCAGCAGCTC CTGGCAGACG AGTCGGCCCC CAGCCCAGGG CACCCCGTGG 
CCCCCCCAGG CCAAGCCGCC CCCCAAAGCC AGTGTACCGC CAAGGCCTAA CTACACCTCC 
AGCTTCAGTG TGATCGGTAG CCGGGAGGAG CGGGGGGTCC GCGCCCCCAG CTTTGGTCCA 
AAGCCGAGGG TGTCAGAGAA CGACTTTGAA GACCTGCTCT CCAACCAGGG CTTCTCCTCG 
CGGGCTGACA GGAAGGGGCC GCGGACCATC GCTGAGATGA GGAGGCAGGA CCAGGCCCGG 
GACTTGGATC CGCTGAAGCT GAAGCTTCTG GAGTGGACGG AGGGCAAGGA GAGGAACATC 
CGCGCCCTGC TGTCCACGCT GCACACGGCG CTGTGGGACG GCGAGAGCCG CTGGACGCCG 
GTGGGCATGG CCGACCTGGT GACGCCCGCA CAAGTGAAGA AGCACTACCG CCGTGCCGTG 
CTGGTGGTCC ACCCCGACAA GGCCCGGGGG CAGCCCTACG AGCAGTATGC CCGGATGATC 
TTCATGGAGC TCAACGATGC GTGGGCTGAG TTTGAGAGCC AGGGCTCCCG GCCACTCTTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAK ( XM_005208283.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005208340.2 Bos taurus cyclin G associated kinase (GAK), transcript variant X3, mRNA.

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CloneID	OBa235258
Clone ID Related Accession (Same CDS sequence)	XM_005208283.2 , XM_024993152.1 , XM_024993153.1
Accession Version	XM_005208283.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3441bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	cyclin-G-associated kinase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005208283.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTCT GCAGAGGGCA GCTGGTGGAA TTTTTAAAAA AAGTTGAATC GAAAGGTCCG 
CTCTCGTGCG ACACCGTCCT GAAGATCTTC TACCAGACGT GCCGGGCGGT GCAGCACATG 
CACAGACAGA AGCCGCCCAT CATCCACAGG GACCTCAAGG TTGAAAACTT ACTGCTTAGT 
AACCAGGGGA CCATCAAGCT GTGTGACTTC GGCAGTGCCA CGACCATCTC ACACTACCCA 
GACTACAGCT GGAGCGCCCA GCGCCGGGCC CTGGTGGAGG AGGAGATCAC CAGGAACACG 
ACCCCCATGT ACAGGACGCC GGAAATTGTG GACTTGTACT CAAACTTTCC GATTGGCGAG 
AAGCAGGACA TCTGGGCCCT GGGCTGCATC CTGTACCTGC TCTGCTTCGG GCAGCACCCC 
TTTGAGGACG GAGCGAAGCT TCGCATCGTC AACGGGAAGT ACGCAATCCC TGCCGACGAC 
ACGCGGTACT CCGTGTTCCA CAGCCTCATC CGTTCCATGC TCAAGGTCAA CCCGGAGGAG 
CGGCTGTCCG TCACCGAGCT GGTCAACCAG CTCCAGGAGA TCGCGGCCGC CCGCAACGTG 
AACCCCAAGT CACCCATCAC TGAGCTCCTG GAGCAGAATG GAGGCTATGG GCACGCGGCC 
CCTCCCGGCT CCACGGGCAG CAGTCTGGTG GTCACTGAGT ACGAGCAGCC CTATGGCGGG 
TTCCTGGACA TTCTGCGTGG GGGCACCGAG CGCCTCTTCA CCAACATCAA GGACACCTCC 
TCCAAAGTCA TCCAGTCCGT TGCCAACTAT GCGAAGGGAG ATCTGGATAT TTCTTACATC 
ACGTCTAGAA TCGCAGTCAT GTCGTTCCCG GCGGAGGGCG TGGAGTCGGC CATCAAGAAC 
AACATTGAGG ATGTACGGCT GTTCCTGGAC TCGAAGCACC CGGGCCGCTA CGCCGTCTAC 
AACCTGTCCC CGCGGACCTA CCGGCCCTCG AAGTTCCACA ACCGGGTCTC CGACTGCGGC 
TGGGTGGCCC GGCGGGCCCC GAGCCTCCGC AGCCTGTACA GCCTCTGCAG GAACATGCAC 
GCCTGGCTGC GCCGCGACCC TGGCAACGTC TGCGTGGTGC ACTGCATGGA CGGGAGGGCG 
GTGTCGGCCG TGGCCGTGTG CTCCTTCTTG TGCTTCTGCC GGCTCTTCAG CACCGCGGAG 
GCCGCTGTGT ACATGTTCAG CATGAAGCGG TGCCCACCGG GCATCTGGCC ATCCCACAAG 
AGGTACATTG AGTACATGTG TGACATGGTG GCCGAGGAGC CCGTCACGCC CCACAGCAAG 
CCCATCCTGG TGAAGGCCGT GGTCATGACG CCTGTGCCGC TCTTCAGCAA GCAGAGGAGC 
GGCTGCAGGC CCTTCTGCGA GGTCTACGTG GGGGATGAGC GCGTGACCAC CACGTCCCAG 
GAATACGACA AGATGAGAGA CTTTAAAATT GAGGATGGCA AAGCGGTCAT TCCCCTGGGC 
ATAACAGTCC AGGGGGACGT GCTCATCATC ATCTATCATG CGAGGTCCAC CCTGGGAGGA 
AGGCTGCAGG CCAAGATGGC GTCCATGAAG ATGTTTCAGA TCCAGTTTCA CACAGGGTTC 
GTGCCTCGGA ACGCAGCTAC TGTGAAGTTT GCCAAGTATG ATCTGGATGC TTGCGACATC 
CAGGAGAAGT ACCCCGACTT GTTCCAAGTG AGCCTGGAGG TGGAGGTGGA GCCCAGAGAC 
AGGCCCGGCC ACGAGGCCCC GCCCTGGGAG GGTGCAGGCA TGCGAGGGCT GAACCCCAAG 
ATCCTCTTCT CCAGCCGGGA GGAGCAGCAA GATGTCCTGT CCAAGTTTGG GAAGCCAGAG 
CTCCCCAGGC AGCCTGGGTC CACCGCACAG TACGACGCCG AGGCCGGGCC CGCGGATGCC 
GAGCCCCCCG AGTCAGACTC GCCGCACAGC AGCGTGGATG CTGACCACTT CCTGCACACG 
CTGGACTGGC GGGAGGATCG GGACTCCAGT GTGGGCCCGG AGGGTCACCT GGCCGAGGAG 
GGTCCCTCTG CTGTGGCCGA GGACGGCTGT GTGAGTGAGC CATCGGACGA GGAAGCCCCG 
ACACCCTCTG CGGAGAGCGG GGACACAGGC GATGAGGACG TGCCCGCCCC CGCCGCCCCG 
CAGGACGACA GCGTGGACCT TCTGGGGCTG CACGCGGAGG CGGGGCCTGT GCCCGCCCCC 
CAGGCCCCCG GCGGCCCCCC CAGCAATGCA GACCTGCTCG GCTGCCTGCT CGGGGCCCCG 
GGCCCCGCTC CGGAGGGTGC TGTGGGGGGC CTGCTCGACG GGGATGCGGC CCTGGTCTTC 
AGCAGCCCAG CCCCCGCGCC TTGCACCCAG ACAACTGCCC CGGCCGCTGA CCCGTTCGGC 
CCGCTCCTGC TGCCGCCCGA CGTGGACACC CCTCCTAGCT CGCAGCCTGA CCTCTTCGGG 
GAATTCCTCA ACTCAGACGC CCCGGCCGCC CAGCCCGCGC CCTTCCCCTC CACCCACAGC 
GCTCCGCCGC CAGCCTGCAG CACCGACTTC CTGCATCTTG GGGACCTGCC CGCCGAGCCC 
AGCAAGATGA CAGCCTCTGC CAGCCACCCC GACCTGCTGG GAGCCTGGGA TGCCTGGGCT 
GAGGCGCCGG CCCCCGCGCT GGCTGCAGAC GGCACCTTCT TCTCTCCTGG AGACCAGCCA 
GCCCCTTCTG GCCTTCCGGC TGGCCAGACC AAGTCTCAGA GCCAGGACCC GTTTGCAGAC 
CTTGGCGACC TCAGTGCCGG CCTCCAAGGC TCGCCTGCCG CAGCTGCTCC TGGGGGCTTT 
GGTCCCAAAG CAGCGCCCCC TCCCAAGGCC AGCAGCTCCT GGCAGACGAG TCGGCCCCCA 
GCCCAGGGCA CCCCGTGGCC CCCCCAGGCC AAGCCGCCCC CCAAAGCCAG TGTACCGCCA 
AGGCCTAACT ACACCTCCAG CTTCAGTGTG ATCGGTAGCC GGGAGGAGCG GGGGGTCCGC 
GCCCCCAGCT TTGGTCCAAA GCCGAGGGTG TCAGAGAACG ACTTTGAAGA CCTGCTCTCC 
AACCAGGGCT TCTCCTCGCG GGCTGACAGG AAGGGGCCGC GGACCATCGC TGAGATGAGG 
AGGCAGGACC AGGCCCGGGA CTTGGATCCG CTGAAGCTGA AGCTTCTGGA GTGGACGGAG 
GGCAAGGAGA GGAACATCCG CGCCCTGCTG TCCACGCTGC ACACGGCGCT GTGGGACGGC 
GAGAGCCGCT GGACGCCGGT GGGCATGGCC GACCTGGTGA CGCCCGCACA AGTGAAGAAG 
CACTACCGCC GTGCCGTGCT GGTGGTCCAC CCCGACAAGG CCCGGGGGCA GCCCTACGAG 
CAGTATGCCC GGATGATCTT CATGGAGCTC AACGATGCGT GGGCTGAGTT TGAGAGCCAG 
GGCTCCCGGC CACTCTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005208340.2
CDS	726..4166
Translation	MELCRGQLVEFLKKVESKGPLSCDTVLKIFYQTCRAVQHMHRQKPPIIHRDLKVENLLLSNQGTIKLCDFGSATTISHYPDYSWSAQRRALVEEEITRNTTPMYRTPEIVDLYSNFPIGEKQDIWALGCILYLLCFGQHPFEDGAKLRIVNGKYAIPADDTRYSVFHSLIRSMLKVNPEERLSVTELVNQLQEIAAARNVNPKSPITELLEQNGGYGHAAPPGSTGSSLVVTEYEQPYGGFLDILRGGTERLFTNIKDTSSKVIQSVANYAKGDLDISYITSRIAVMSFPAEGVESAIKNNIEDVRLFLDSKHPGRYAVYNLSPRTYRPSKFHNRVSDCGWVARRAPSLRSLYSLCRNMHAWLRRDPGNVCVVHCMDGRAVSAVAVCSFLCFCRLFSTAEAAVYMFSMKRCPPGIWPSHKRYIEYMCDMVAEEPVTPHSKPILVKAVVMTPVPLFSKQRSGCRPFCEVYVGDERVTTTSQEYDKMRDFKIEDGKAVIPLGITVQGDVLIIIYHARSTLGGRLQAKMASMKMFQIQFHTGFVPRNAATVKFAKYDLDACDIQEKYPDLFQVSLEVEVEPRDRPGHEAPPWEGAGMRGLNPKILFSSREEQQDVLSKFGKPELPRQPGSTAQYDAEAGPADAEPPESDSPHSSVDADHFLHTLDWREDRDSSVGPEGHLAEEGPSAVAEDGCVSEPSDEEAPTPSAESGDTGDEDVPAPAAPQDDSVDLLGLHAEAGPVPAPQAPGGPPSNADLLGCLLGAPGPAPEGAVGGLLDGDAALVFSSPAPAPCTQTTAPAADPFGPLLLPPDVDTPPSSQPDLFGEFLNSDAPAAQPAPFPSTHSAPPPACSTDFLHLGDLPAEPSKMTASASHPDLLGAWDAWAEAPAPALAADGTFFSPGDQPAPSGLPAGQTKSQSQDPFADLGDLSAGLQGSPAAAAPGGFGPKAAPPPKASSSWQTSRPPAQGTPWPPQAKPPPKASVPPRPNYTSSFSVIGSREERGVRAPSFGPKPRVSENDFEDLLSNQGFSSRADRKGPRTIAEMRRQDQARDLDPLKLKLLEWTEGKERNIRALLSTLHTALWDGESRWTPVGMADLVTPAQVKKHYRRAVLVVHPDKARGQPYEQYARMIFMELNDAWAEFESQGSRPLF

Target ORF information:

RefSeq Version	XM_005208283.2
Organism	Bos taurus(cattle)
Definition	Bos taurus cyclin G associated kinase (GAK), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005208283.2

ORF Insert Sequence:

ATGGAGCTCT GCAGAGGGCA GCTGGTGGAA TTTTTAAAAA AAGTTGAATC GAAAGGTCCG 
CTCTCGTGCG ACACCGTCCT GAAGATCTTC TACCAGACGT GCCGGGCGGT GCAGCACATG 
CACAGACAGA AGCCGCCCAT CATCCACAGG GACCTCAAGG TTGAAAACTT ACTGCTTAGT 
AACCAGGGGA CCATCAAGCT GTGTGACTTC GGCAGTGCCA CGACCATCTC ACACTACCCA 
GACTACAGCT GGAGCGCCCA GCGCCGGGCC CTGGTGGAGG AGGAGATCAC CAGGAACACG 
ACCCCCATGT ACAGGACGCC GGAAATTGTG GACTTGTACT CAAACTTTCC GATTGGCGAG 
AAGCAGGACA TCTGGGCCCT GGGCTGCATC CTGTACCTGC TCTGCTTCGG GCAGCACCCC 
TTTGAGGACG GAGCGAAGCT TCGCATCGTC AACGGGAAGT ACGCAATCCC TGCCGACGAC 
ACGCGGTACT CCGTGTTCCA CAGCCTCATC CGTTCCATGC TCAAGGTCAA CCCGGAGGAG 
CGGCTGTCCG TCACCGAGCT GGTCAACCAG CTCCAGGAGA TCGCGGCCGC CCGCAACGTG 
AACCCCAAGT CACCCATCAC TGAGCTCCTG GAGCAGAATG GAGGCTATGG GCACGCGGCC 
CCTCCCGGCT CCACGGGCAG CAGTCTGGTG GTCACTGAGT ACGAGCAGCC CTATGGCGGG 
TTCCTGGACA TTCTGCGTGG GGGCACCGAG CGCCTCTTCA CCAACATCAA GGACACCTCC 
TCCAAAGTCA TCCAGTCCGT TGCCAACTAT GCGAAGGGAG ATCTGGATAT TTCTTACATC 
ACGTCTAGAA TCGCAGTCAT GTCGTTCCCG GCGGAGGGCG TGGAGTCGGC CATCAAGAAC 
AACATTGAGG ATGTACGGCT GTTCCTGGAC TCGAAGCACC CGGGCCGCTA CGCCGTCTAC 
AACCTGTCCC CGCGGACCTA CCGGCCCTCG AAGTTCCACA ACCGGGTCTC CGACTGCGGC 
TGGGTGGCCC GGCGGGCCCC GAGCCTCCGC AGCCTGTACA GCCTCTGCAG GAACATGCAC 
GCCTGGCTGC GCCGCGACCC TGGCAACGTC TGCGTGGTGC ACTGCATGGA CGGGAGGGCG 
GTGTCGGCCG TGGCCGTGTG CTCCTTCTTG TGCTTCTGCC GGCTCTTCAG CACCGCGGAG 
GCCGCTGTGT ACATGTTCAG CATGAAGCGG TGCCCACCGG GCATCTGGCC ATCCCACAAG 
AGGTACATTG AGTACATGTG TGACATGGTG GCCGAGGAGC CCGTCACGCC CCACAGCAAG 
CCCATCCTGG TGAAGGCCGT GGTCATGACG CCTGTGCCGC TCTTCAGCAA GCAGAGGAGC 
GGCTGCAGGC CCTTCTGCGA GGTCTACGTG GGGGATGAGC GCGTGACCAC CACGTCCCAG 
GAATACGACA AGATGAGAGA CTTTAAAATT GAGGATGGCA AAGCGGTCAT TCCCCTGGGC 
ATAACAGTCC AGGGGGACGT GCTCATCATC ATCTATCATG CGAGGTCCAC CCTGGGAGGA 
AGGCTGCAGG CCAAGATGGC GTCCATGAAG ATGTTTCAGA TCCAGTTTCA CACAGGGTTC 
GTGCCTCGGA ACGCAGCTAC TGTGAAGTTT GCCAAGTATG ATCTGGATGC TTGCGACATC 
CAGGAGAAGT ACCCCGACTT GTTCCAAGTG AGCCTGGAGG TGGAGGTGGA GCCCAGAGAC 
AGGCCCGGCC ACGAGGCCCC GCCCTGGGAG GGTGCAGGCA TGCGAGGGCT GAACCCCAAG 
ATCCTCTTCT CCAGCCGGGA GGAGCAGCAA GATGTCCTGT CCAAGTTTGG GAAGCCAGAG 
CTCCCCAGGC AGCCTGGGTC CACCGCACAG TACGACGCCG AGGCCGGGCC CGCGGATGCC 
GAGCCCCCCG AGTCAGACTC GCCGCACAGC AGCGTGGATG CTGACCACTT CCTGCACACG 
CTGGACTGGC GGGAGGATCG GGACTCCAGT GTGGGCCCGG AGGGTCACCT GGCCGAGGAG 
GGTCCCTCTG CTGTGGCCGA GGACGGCTGT GTGAGTGAGC CATCGGACGA GGAAGCCCCG 
ACACCCTCTG CGGAGAGCGG GGACACAGGC GATGAGGACG TGCCCGCCCC CGCCGCCCCG 
CAGGACGACA GCGTGGACCT TCTGGGGCTG CACGCGGAGG CGGGGCCTGT GCCCGCCCCC 
CAGGCCCCCG GCGGCCCCCC CAGCAATGCA GACCTGCTCG GCTGCCTGCT CGGGGCCCCG 
GGCCCCGCTC CGGAGGGTGC TGTGGGGGGC CTGCTCGACG GGGATGCGGC CCTGGTCTTC 
AGCAGCCCAG CCCCCGCGCC TTGCACCCAG ACAACTGCCC CGGCCGCTGA CCCGTTCGGC 
CCGCTCCTGC TGCCGCCCGA CGTGGACACC CCTCCTAGCT CGCAGCCTGA CCTCTTCGGG 
GAATTCCTCA ACTCAGACGC CCCGGCCGCC CAGCCCGCGC CCTTCCCCTC CACCCACAGC 
GCTCCGCCGC CAGCCTGCAG CACCGACTTC CTGCATCTTG GGGACCTGCC CGCCGAGCCC 
AGCAAGATGA CAGCCTCTGC CAGCCACCCC GACCTGCTGG GAGCCTGGGA TGCCTGGGCT 
GAGGCGCCGG CCCCCGCGCT GGCTGCAGAC GGCACCTTCT TCTCTCCTGG AGACCAGCCA 
GCCCCTTCTG GCCTTCCGGC TGGCCAGACC AAGTCTCAGA GCCAGGACCC GTTTGCAGAC 
CTTGGCGACC TCAGTGCCGG CCTCCAAGGC TCGCCTGCCG CAGCTGCTCC TGGGGGCTTT 
GGTCCCAAAG CAGCGCCCCC TCCCAAGGCC AGCAGCTCCT GGCAGACGAG TCGGCCCCCA 
GCCCAGGGCA CCCCGTGGCC CCCCCAGGCC AAGCCGCCCC CCAAAGCCAG TGTACCGCCA 
AGGCCTAACT ACACCTCCAG CTTCAGTGTG ATCGGTAGCC GGGAGGAGCG GGGGGTCCGC 
GCCCCCAGCT TTGGTCCAAA GCCGAGGGTG TCAGAGAACG ACTTTGAAGA CCTGCTCTCC 
AACCAGGGCT TCTCCTCGCG GGCTGACAGG AAGGGGCCGC GGACCATCGC TGAGATGAGG 
AGGCAGGACC AGGCCCGGGA CTTGGATCCG CTGAAGCTGA AGCTTCTGGA GTGGACGGAG 
GGCAAGGAGA GGAACATCCG CGCCCTGCTG TCCACGCTGC ACACGGCGCT GTGGGACGGC 
GAGAGCCGCT GGACGCCGGT GGGCATGGCC GACCTGGTGA CGCCCGCACA AGTGAAGAAG 
CACTACCGCC GTGCCGTGCT GGTGGTCCAC CCCGACAAGG CCCGGGGGCA GCCCTACGAG 
CAGTATGCCC GGATGATCTT CATGGAGCTC AACGATGCGT GGGCTGAGTT TGAGAGCCAG 
GGCTCCCGGC CACTCTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAK ( XM_024993152.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024848920.1 Bos taurus cyclin G associated kinase (GAK), transcript variant X4, mRNA.

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CloneID	OBa235258
Clone ID Related Accession (Same CDS sequence)	XM_005208283.2 , XM_024993152.1 , XM_024993153.1
Accession Version	XM_024993152.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3441bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	cyclin-G-associated kinase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTCT GCAGAGGGCA GCTGGTGGAA TTTTTAAAAA AAGTTGAATC GAAAGGTCCG 
CTCTCGTGCG ACACCGTCCT GAAGATCTTC TACCAGACGT GCCGGGCGGT GCAGCACATG 
CACAGACAGA AGCCGCCCAT CATCCACAGG GACCTCAAGG TTGAAAACTT ACTGCTTAGT 
AACCAGGGGA CCATCAAGCT GTGTGACTTC GGCAGTGCCA CGACCATCTC ACACTACCCA 
GACTACAGCT GGAGCGCCCA GCGCCGGGCC CTGGTGGAGG AGGAGATCAC CAGGAACACG 
ACCCCCATGT ACAGGACGCC GGAAATTGTG GACTTGTACT CAAACTTTCC GATTGGCGAG 
AAGCAGGACA TCTGGGCCCT GGGCTGCATC CTGTACCTGC TCTGCTTCGG GCAGCACCCC 
TTTGAGGACG GAGCGAAGCT TCGCATCGTC AACGGGAAGT ACGCAATCCC TGCCGACGAC 
ACGCGGTACT CCGTGTTCCA CAGCCTCATC CGTTCCATGC TCAAGGTCAA CCCGGAGGAG 
CGGCTGTCCG TCACCGAGCT GGTCAACCAG CTCCAGGAGA TCGCGGCCGC CCGCAACGTG 
AACCCCAAGT CACCCATCAC TGAGCTCCTG GAGCAGAATG GAGGCTATGG GCACGCGGCC 
CCTCCCGGCT CCACGGGCAG CAGTCTGGTG GTCACTGAGT ACGAGCAGCC CTATGGCGGG 
TTCCTGGACA TTCTGCGTGG GGGCACCGAG CGCCTCTTCA CCAACATCAA GGACACCTCC 
TCCAAAGTCA TCCAGTCCGT TGCCAACTAT GCGAAGGGAG ATCTGGATAT TTCTTACATC 
ACGTCTAGAA TCGCAGTCAT GTCGTTCCCG GCGGAGGGCG TGGAGTCGGC CATCAAGAAC 
AACATTGAGG ATGTACGGCT GTTCCTGGAC TCGAAGCACC CGGGCCGCTA CGCCGTCTAC 
AACCTGTCCC CGCGGACCTA CCGGCCCTCG AAGTTCCACA ACCGGGTCTC CGACTGCGGC 
TGGGTGGCCC GGCGGGCCCC GAGCCTCCGC AGCCTGTACA GCCTCTGCAG GAACATGCAC 
GCCTGGCTGC GCCGCGACCC TGGCAACGTC TGCGTGGTGC ACTGCATGGA CGGGAGGGCG 
GTGTCGGCCG TGGCCGTGTG CTCCTTCTTG TGCTTCTGCC GGCTCTTCAG CACCGCGGAG 
GCCGCTGTGT ACATGTTCAG CATGAAGCGG TGCCCACCGG GCATCTGGCC ATCCCACAAG 
AGGTACATTG AGTACATGTG TGACATGGTG GCCGAGGAGC CCGTCACGCC CCACAGCAAG 
CCCATCCTGG TGAAGGCCGT GGTCATGACG CCTGTGCCGC TCTTCAGCAA GCAGAGGAGC 
GGCTGCAGGC CCTTCTGCGA GGTCTACGTG GGGGATGAGC GCGTGACCAC CACGTCCCAG 
GAATACGACA AGATGAGAGA CTTTAAAATT GAGGATGGCA AAGCGGTCAT TCCCCTGGGC 
ATAACAGTCC AGGGGGACGT GCTCATCATC ATCTATCATG CGAGGTCCAC CCTGGGAGGA 
AGGCTGCAGG CCAAGATGGC GTCCATGAAG ATGTTTCAGA TCCAGTTTCA CACAGGGTTC 
GTGCCTCGGA ACGCAGCTAC TGTGAAGTTT GCCAAGTATG ATCTGGATGC TTGCGACATC 
CAGGAGAAGT ACCCCGACTT GTTCCAAGTG AGCCTGGAGG TGGAGGTGGA GCCCAGAGAC 
AGGCCCGGCC ACGAGGCCCC GCCCTGGGAG GGTGCAGGCA TGCGAGGGCT GAACCCCAAG 
ATCCTCTTCT CCAGCCGGGA GGAGCAGCAA GATGTCCTGT CCAAGTTTGG GAAGCCAGAG 
CTCCCCAGGC AGCCTGGGTC CACCGCACAG TACGACGCCG AGGCCGGGCC CGCGGATGCC 
GAGCCCCCCG AGTCAGACTC GCCGCACAGC AGCGTGGATG CTGACCACTT CCTGCACACG 
CTGGACTGGC GGGAGGATCG GGACTCCAGT GTGGGCCCGG AGGGTCACCT GGCCGAGGAG 
GGTCCCTCTG CTGTGGCCGA GGACGGCTGT GTGAGTGAGC CATCGGACGA GGAAGCCCCG 
ACACCCTCTG CGGAGAGCGG GGACACAGGC GATGAGGACG TGCCCGCCCC CGCCGCCCCG 
CAGGACGACA GCGTGGACCT TCTGGGGCTG CACGCGGAGG CGGGGCCTGT GCCCGCCCCC 
CAGGCCCCCG GCGGCCCCCC CAGCAATGCA GACCTGCTCG GCTGCCTGCT CGGGGCCCCG 
GGCCCCGCTC CGGAGGGTGC TGTGGGGGGC CTGCTCGACG GGGATGCGGC CCTGGTCTTC 
AGCAGCCCAG CCCCCGCGCC TTGCACCCAG ACAACTGCCC CGGCCGCTGA CCCGTTCGGC 
CCGCTCCTGC TGCCGCCCGA CGTGGACACC CCTCCTAGCT CGCAGCCTGA CCTCTTCGGG 
GAATTCCTCA ACTCAGACGC CCCGGCCGCC CAGCCCGCGC CCTTCCCCTC CACCCACAGC 
GCTCCGCCGC CAGCCTGCAG CACCGACTTC CTGCATCTTG GGGACCTGCC CGCCGAGCCC 
AGCAAGATGA CAGCCTCTGC CAGCCACCCC GACCTGCTGG GAGCCTGGGA TGCCTGGGCT 
GAGGCGCCGG CCCCCGCGCT GGCTGCAGAC GGCACCTTCT TCTCTCCTGG AGACCAGCCA 
GCCCCTTCTG GCCTTCCGGC TGGCCAGACC AAGTCTCAGA GCCAGGACCC GTTTGCAGAC 
CTTGGCGACC TCAGTGCCGG CCTCCAAGGC TCGCCTGCCG CAGCTGCTCC TGGGGGCTTT 
GGTCCCAAAG CAGCGCCCCC TCCCAAGGCC AGCAGCTCCT GGCAGACGAG TCGGCCCCCA 
GCCCAGGGCA CCCCGTGGCC CCCCCAGGCC AAGCCGCCCC CCAAAGCCAG TGTACCGCCA 
AGGCCTAACT ACACCTCCAG CTTCAGTGTG ATCGGTAGCC GGGAGGAGCG GGGGGTCCGC 
GCCCCCAGCT TTGGTCCAAA GCCGAGGGTG TCAGAGAACG ACTTTGAAGA CCTGCTCTCC 
AACCAGGGCT TCTCCTCGCG GGCTGACAGG AAGGGGCCGC GGACCATCGC TGAGATGAGG 
AGGCAGGACC AGGCCCGGGA CTTGGATCCG CTGAAGCTGA AGCTTCTGGA GTGGACGGAG 
GGCAAGGAGA GGAACATCCG CGCCCTGCTG TCCACGCTGC ACACGGCGCT GTGGGACGGC 
GAGAGCCGCT GGACGCCGGT GGGCATGGCC GACCTGGTGA CGCCCGCACA AGTGAAGAAG 
CACTACCGCC GTGCCGTGCT GGTGGTCCAC CCCGACAAGG CCCGGGGGCA GCCCTACGAG 
CAGTATGCCC GGATGATCTT CATGGAGCTC AACGATGCGT GGGCTGAGTT TGAGAGCCAG 
GGCTCCCGGC CACTCTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024848920.1
CDS	783..4223
Translation	MELCRGQLVEFLKKVESKGPLSCDTVLKIFYQTCRAVQHMHRQKPPIIHRDLKVENLLLSNQGTIKLCDFGSATTISHYPDYSWSAQRRALVEEEITRNTTPMYRTPEIVDLYSNFPIGEKQDIWALGCILYLLCFGQHPFEDGAKLRIVNGKYAIPADDTRYSVFHSLIRSMLKVNPEERLSVTELVNQLQEIAAARNVNPKSPITELLEQNGGYGHAAPPGSTGSSLVVTEYEQPYGGFLDILRGGTERLFTNIKDTSSKVIQSVANYAKGDLDISYITSRIAVMSFPAEGVESAIKNNIEDVRLFLDSKHPGRYAVYNLSPRTYRPSKFHNRVSDCGWVARRAPSLRSLYSLCRNMHAWLRRDPGNVCVVHCMDGRAVSAVAVCSFLCFCRLFSTAEAAVYMFSMKRCPPGIWPSHKRYIEYMCDMVAEEPVTPHSKPILVKAVVMTPVPLFSKQRSGCRPFCEVYVGDERVTTTSQEYDKMRDFKIEDGKAVIPLGITVQGDVLIIIYHARSTLGGRLQAKMASMKMFQIQFHTGFVPRNAATVKFAKYDLDACDIQEKYPDLFQVSLEVEVEPRDRPGHEAPPWEGAGMRGLNPKILFSSREEQQDVLSKFGKPELPRQPGSTAQYDAEAGPADAEPPESDSPHSSVDADHFLHTLDWREDRDSSVGPEGHLAEEGPSAVAEDGCVSEPSDEEAPTPSAESGDTGDEDVPAPAAPQDDSVDLLGLHAEAGPVPAPQAPGGPPSNADLLGCLLGAPGPAPEGAVGGLLDGDAALVFSSPAPAPCTQTTAPAADPFGPLLLPPDVDTPPSSQPDLFGEFLNSDAPAAQPAPFPSTHSAPPPACSTDFLHLGDLPAEPSKMTASASHPDLLGAWDAWAEAPAPALAADGTFFSPGDQPAPSGLPAGQTKSQSQDPFADLGDLSAGLQGSPAAAAPGGFGPKAAPPPKASSSWQTSRPPAQGTPWPPQAKPPPKASVPPRPNYTSSFSVIGSREERGVRAPSFGPKPRVSENDFEDLLSNQGFSSRADRKGPRTIAEMRRQDQARDLDPLKLKLLEWTEGKERNIRALLSTLHTALWDGESRWTPVGMADLVTPAQVKKHYRRAVLVVHPDKARGQPYEQYARMIFMELNDAWAEFESQGSRPLF

Target ORF information:

RefSeq Version	XM_024993152.1
Organism	Bos taurus(cattle)
Definition	Bos taurus cyclin G associated kinase (GAK), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024993152.1

ORF Insert Sequence:

ATGGAGCTCT GCAGAGGGCA GCTGGTGGAA TTTTTAAAAA AAGTTGAATC GAAAGGTCCG 
CTCTCGTGCG ACACCGTCCT GAAGATCTTC TACCAGACGT GCCGGGCGGT GCAGCACATG 
CACAGACAGA AGCCGCCCAT CATCCACAGG GACCTCAAGG TTGAAAACTT ACTGCTTAGT 
AACCAGGGGA CCATCAAGCT GTGTGACTTC GGCAGTGCCA CGACCATCTC ACACTACCCA 
GACTACAGCT GGAGCGCCCA GCGCCGGGCC CTGGTGGAGG AGGAGATCAC CAGGAACACG 
ACCCCCATGT ACAGGACGCC GGAAATTGTG GACTTGTACT CAAACTTTCC GATTGGCGAG 
AAGCAGGACA TCTGGGCCCT GGGCTGCATC CTGTACCTGC TCTGCTTCGG GCAGCACCCC 
TTTGAGGACG GAGCGAAGCT TCGCATCGTC AACGGGAAGT ACGCAATCCC TGCCGACGAC 
ACGCGGTACT CCGTGTTCCA CAGCCTCATC CGTTCCATGC TCAAGGTCAA CCCGGAGGAG 
CGGCTGTCCG TCACCGAGCT GGTCAACCAG CTCCAGGAGA TCGCGGCCGC CCGCAACGTG 
AACCCCAAGT CACCCATCAC TGAGCTCCTG GAGCAGAATG GAGGCTATGG GCACGCGGCC 
CCTCCCGGCT CCACGGGCAG CAGTCTGGTG GTCACTGAGT ACGAGCAGCC CTATGGCGGG 
TTCCTGGACA TTCTGCGTGG GGGCACCGAG CGCCTCTTCA CCAACATCAA GGACACCTCC 
TCCAAAGTCA TCCAGTCCGT TGCCAACTAT GCGAAGGGAG ATCTGGATAT TTCTTACATC 
ACGTCTAGAA TCGCAGTCAT GTCGTTCCCG GCGGAGGGCG TGGAGTCGGC CATCAAGAAC 
AACATTGAGG ATGTACGGCT GTTCCTGGAC TCGAAGCACC CGGGCCGCTA CGCCGTCTAC 
AACCTGTCCC CGCGGACCTA CCGGCCCTCG AAGTTCCACA ACCGGGTCTC CGACTGCGGC 
TGGGTGGCCC GGCGGGCCCC GAGCCTCCGC AGCCTGTACA GCCTCTGCAG GAACATGCAC 
GCCTGGCTGC GCCGCGACCC TGGCAACGTC TGCGTGGTGC ACTGCATGGA CGGGAGGGCG 
GTGTCGGCCG TGGCCGTGTG CTCCTTCTTG TGCTTCTGCC GGCTCTTCAG CACCGCGGAG 
GCCGCTGTGT ACATGTTCAG CATGAAGCGG TGCCCACCGG GCATCTGGCC ATCCCACAAG 
AGGTACATTG AGTACATGTG TGACATGGTG GCCGAGGAGC CCGTCACGCC CCACAGCAAG 
CCCATCCTGG TGAAGGCCGT GGTCATGACG CCTGTGCCGC TCTTCAGCAA GCAGAGGAGC 
GGCTGCAGGC CCTTCTGCGA GGTCTACGTG GGGGATGAGC GCGTGACCAC CACGTCCCAG 
GAATACGACA AGATGAGAGA CTTTAAAATT GAGGATGGCA AAGCGGTCAT TCCCCTGGGC 
ATAACAGTCC AGGGGGACGT GCTCATCATC ATCTATCATG CGAGGTCCAC CCTGGGAGGA 
AGGCTGCAGG CCAAGATGGC GTCCATGAAG ATGTTTCAGA TCCAGTTTCA CACAGGGTTC 
GTGCCTCGGA ACGCAGCTAC TGTGAAGTTT GCCAAGTATG ATCTGGATGC TTGCGACATC 
CAGGAGAAGT ACCCCGACTT GTTCCAAGTG AGCCTGGAGG TGGAGGTGGA GCCCAGAGAC 
AGGCCCGGCC ACGAGGCCCC GCCCTGGGAG GGTGCAGGCA TGCGAGGGCT GAACCCCAAG 
ATCCTCTTCT CCAGCCGGGA GGAGCAGCAA GATGTCCTGT CCAAGTTTGG GAAGCCAGAG 
CTCCCCAGGC AGCCTGGGTC CACCGCACAG TACGACGCCG AGGCCGGGCC CGCGGATGCC 
GAGCCCCCCG AGTCAGACTC GCCGCACAGC AGCGTGGATG CTGACCACTT CCTGCACACG 
CTGGACTGGC GGGAGGATCG GGACTCCAGT GTGGGCCCGG AGGGTCACCT GGCCGAGGAG 
GGTCCCTCTG CTGTGGCCGA GGACGGCTGT GTGAGTGAGC CATCGGACGA GGAAGCCCCG 
ACACCCTCTG CGGAGAGCGG GGACACAGGC GATGAGGACG TGCCCGCCCC CGCCGCCCCG 
CAGGACGACA GCGTGGACCT TCTGGGGCTG CACGCGGAGG CGGGGCCTGT GCCCGCCCCC 
CAGGCCCCCG GCGGCCCCCC CAGCAATGCA GACCTGCTCG GCTGCCTGCT CGGGGCCCCG 
GGCCCCGCTC CGGAGGGTGC TGTGGGGGGC CTGCTCGACG GGGATGCGGC CCTGGTCTTC 
AGCAGCCCAG CCCCCGCGCC TTGCACCCAG ACAACTGCCC CGGCCGCTGA CCCGTTCGGC 
CCGCTCCTGC TGCCGCCCGA CGTGGACACC CCTCCTAGCT CGCAGCCTGA CCTCTTCGGG 
GAATTCCTCA ACTCAGACGC CCCGGCCGCC CAGCCCGCGC CCTTCCCCTC CACCCACAGC 
GCTCCGCCGC CAGCCTGCAG CACCGACTTC CTGCATCTTG GGGACCTGCC CGCCGAGCCC 
AGCAAGATGA CAGCCTCTGC CAGCCACCCC GACCTGCTGG GAGCCTGGGA TGCCTGGGCT 
GAGGCGCCGG CCCCCGCGCT GGCTGCAGAC GGCACCTTCT TCTCTCCTGG AGACCAGCCA 
GCCCCTTCTG GCCTTCCGGC TGGCCAGACC AAGTCTCAGA GCCAGGACCC GTTTGCAGAC 
CTTGGCGACC TCAGTGCCGG CCTCCAAGGC TCGCCTGCCG CAGCTGCTCC TGGGGGCTTT 
GGTCCCAAAG CAGCGCCCCC TCCCAAGGCC AGCAGCTCCT GGCAGACGAG TCGGCCCCCA 
GCCCAGGGCA CCCCGTGGCC CCCCCAGGCC AAGCCGCCCC CCAAAGCCAG TGTACCGCCA 
AGGCCTAACT ACACCTCCAG CTTCAGTGTG ATCGGTAGCC GGGAGGAGCG GGGGGTCCGC 
GCCCCCAGCT TTGGTCCAAA GCCGAGGGTG TCAGAGAACG ACTTTGAAGA CCTGCTCTCC 
AACCAGGGCT TCTCCTCGCG GGCTGACAGG AAGGGGCCGC GGACCATCGC TGAGATGAGG 
AGGCAGGACC AGGCCCGGGA CTTGGATCCG CTGAAGCTGA AGCTTCTGGA GTGGACGGAG 
GGCAAGGAGA GGAACATCCG CGCCCTGCTG TCCACGCTGC ACACGGCGCT GTGGGACGGC 
GAGAGCCGCT GGACGCCGGT GGGCATGGCC GACCTGGTGA CGCCCGCACA AGTGAAGAAG 
CACTACCGCC GTGCCGTGCT GGTGGTCCAC CCCGACAAGG CCCGGGGGCA GCCCTACGAG 
CAGTATGCCC GGATGATCTT CATGGAGCTC AACGATGCGT GGGCTGAGTT TGAGAGCCAG 
GGCTCCCGGC CACTCTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAK ( XM_024993153.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024848921.1 Bos taurus cyclin G associated kinase (GAK), transcript variant X5, mRNA.

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CloneID	OBa235258
Clone ID Related Accession (Same CDS sequence)	XM_005208283.2 , XM_024993152.1 , XM_024993153.1
Accession Version	XM_024993153.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3441bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	cyclin-G-associated kinase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTCT GCAGAGGGCA GCTGGTGGAA TTTTTAAAAA AAGTTGAATC GAAAGGTCCG 
CTCTCGTGCG ACACCGTCCT GAAGATCTTC TACCAGACGT GCCGGGCGGT GCAGCACATG 
CACAGACAGA AGCCGCCCAT CATCCACAGG GACCTCAAGG TTGAAAACTT ACTGCTTAGT 
AACCAGGGGA CCATCAAGCT GTGTGACTTC GGCAGTGCCA CGACCATCTC ACACTACCCA 
GACTACAGCT GGAGCGCCCA GCGCCGGGCC CTGGTGGAGG AGGAGATCAC CAGGAACACG 
ACCCCCATGT ACAGGACGCC GGAAATTGTG GACTTGTACT CAAACTTTCC GATTGGCGAG 
AAGCAGGACA TCTGGGCCCT GGGCTGCATC CTGTACCTGC TCTGCTTCGG GCAGCACCCC 
TTTGAGGACG GAGCGAAGCT TCGCATCGTC AACGGGAAGT ACGCAATCCC TGCCGACGAC 
ACGCGGTACT CCGTGTTCCA CAGCCTCATC CGTTCCATGC TCAAGGTCAA CCCGGAGGAG 
CGGCTGTCCG TCACCGAGCT GGTCAACCAG CTCCAGGAGA TCGCGGCCGC CCGCAACGTG 
AACCCCAAGT CACCCATCAC TGAGCTCCTG GAGCAGAATG GAGGCTATGG GCACGCGGCC 
CCTCCCGGCT CCACGGGCAG CAGTCTGGTG GTCACTGAGT ACGAGCAGCC CTATGGCGGG 
TTCCTGGACA TTCTGCGTGG GGGCACCGAG CGCCTCTTCA CCAACATCAA GGACACCTCC 
TCCAAAGTCA TCCAGTCCGT TGCCAACTAT GCGAAGGGAG ATCTGGATAT TTCTTACATC 
ACGTCTAGAA TCGCAGTCAT GTCGTTCCCG GCGGAGGGCG TGGAGTCGGC CATCAAGAAC 
AACATTGAGG ATGTACGGCT GTTCCTGGAC TCGAAGCACC CGGGCCGCTA CGCCGTCTAC 
AACCTGTCCC CGCGGACCTA CCGGCCCTCG AAGTTCCACA ACCGGGTCTC CGACTGCGGC 
TGGGTGGCCC GGCGGGCCCC GAGCCTCCGC AGCCTGTACA GCCTCTGCAG GAACATGCAC 
GCCTGGCTGC GCCGCGACCC TGGCAACGTC TGCGTGGTGC ACTGCATGGA CGGGAGGGCG 
GTGTCGGCCG TGGCCGTGTG CTCCTTCTTG TGCTTCTGCC GGCTCTTCAG CACCGCGGAG 
GCCGCTGTGT ACATGTTCAG CATGAAGCGG TGCCCACCGG GCATCTGGCC ATCCCACAAG 
AGGTACATTG AGTACATGTG TGACATGGTG GCCGAGGAGC CCGTCACGCC CCACAGCAAG 
CCCATCCTGG TGAAGGCCGT GGTCATGACG CCTGTGCCGC TCTTCAGCAA GCAGAGGAGC 
GGCTGCAGGC CCTTCTGCGA GGTCTACGTG GGGGATGAGC GCGTGACCAC CACGTCCCAG 
GAATACGACA AGATGAGAGA CTTTAAAATT GAGGATGGCA AAGCGGTCAT TCCCCTGGGC 
ATAACAGTCC AGGGGGACGT GCTCATCATC ATCTATCATG CGAGGTCCAC CCTGGGAGGA 
AGGCTGCAGG CCAAGATGGC GTCCATGAAG ATGTTTCAGA TCCAGTTTCA CACAGGGTTC 
GTGCCTCGGA ACGCAGCTAC TGTGAAGTTT GCCAAGTATG ATCTGGATGC TTGCGACATC 
CAGGAGAAGT ACCCCGACTT GTTCCAAGTG AGCCTGGAGG TGGAGGTGGA GCCCAGAGAC 
AGGCCCGGCC ACGAGGCCCC GCCCTGGGAG GGTGCAGGCA TGCGAGGGCT GAACCCCAAG 
ATCCTCTTCT CCAGCCGGGA GGAGCAGCAA GATGTCCTGT CCAAGTTTGG GAAGCCAGAG 
CTCCCCAGGC AGCCTGGGTC CACCGCACAG TACGACGCCG AGGCCGGGCC CGCGGATGCC 
GAGCCCCCCG AGTCAGACTC GCCGCACAGC AGCGTGGATG CTGACCACTT CCTGCACACG 
CTGGACTGGC GGGAGGATCG GGACTCCAGT GTGGGCCCGG AGGGTCACCT GGCCGAGGAG 
GGTCCCTCTG CTGTGGCCGA GGACGGCTGT GTGAGTGAGC CATCGGACGA GGAAGCCCCG 
ACACCCTCTG CGGAGAGCGG GGACACAGGC GATGAGGACG TGCCCGCCCC CGCCGCCCCG 
CAGGACGACA GCGTGGACCT TCTGGGGCTG CACGCGGAGG CGGGGCCTGT GCCCGCCCCC 
CAGGCCCCCG GCGGCCCCCC CAGCAATGCA GACCTGCTCG GCTGCCTGCT CGGGGCCCCG 
GGCCCCGCTC CGGAGGGTGC TGTGGGGGGC CTGCTCGACG GGGATGCGGC CCTGGTCTTC 
AGCAGCCCAG CCCCCGCGCC TTGCACCCAG ACAACTGCCC CGGCCGCTGA CCCGTTCGGC 
CCGCTCCTGC TGCCGCCCGA CGTGGACACC CCTCCTAGCT CGCAGCCTGA CCTCTTCGGG 
GAATTCCTCA ACTCAGACGC CCCGGCCGCC CAGCCCGCGC CCTTCCCCTC CACCCACAGC 
GCTCCGCCGC CAGCCTGCAG CACCGACTTC CTGCATCTTG GGGACCTGCC CGCCGAGCCC 
AGCAAGATGA CAGCCTCTGC CAGCCACCCC GACCTGCTGG GAGCCTGGGA TGCCTGGGCT 
GAGGCGCCGG CCCCCGCGCT GGCTGCAGAC GGCACCTTCT TCTCTCCTGG AGACCAGCCA 
GCCCCTTCTG GCCTTCCGGC TGGCCAGACC AAGTCTCAGA GCCAGGACCC GTTTGCAGAC 
CTTGGCGACC TCAGTGCCGG CCTCCAAGGC TCGCCTGCCG CAGCTGCTCC TGGGGGCTTT 
GGTCCCAAAG CAGCGCCCCC TCCCAAGGCC AGCAGCTCCT GGCAGACGAG TCGGCCCCCA 
GCCCAGGGCA CCCCGTGGCC CCCCCAGGCC AAGCCGCCCC CCAAAGCCAG TGTACCGCCA 
AGGCCTAACT ACACCTCCAG CTTCAGTGTG ATCGGTAGCC GGGAGGAGCG GGGGGTCCGC 
GCCCCCAGCT TTGGTCCAAA GCCGAGGGTG TCAGAGAACG ACTTTGAAGA CCTGCTCTCC 
AACCAGGGCT TCTCCTCGCG GGCTGACAGG AAGGGGCCGC GGACCATCGC TGAGATGAGG 
AGGCAGGACC AGGCCCGGGA CTTGGATCCG CTGAAGCTGA AGCTTCTGGA GTGGACGGAG 
GGCAAGGAGA GGAACATCCG CGCCCTGCTG TCCACGCTGC ACACGGCGCT GTGGGACGGC 
GAGAGCCGCT GGACGCCGGT GGGCATGGCC GACCTGGTGA CGCCCGCACA AGTGAAGAAG 
CACTACCGCC GTGCCGTGCT GGTGGTCCAC CCCGACAAGG CCCGGGGGCA GCCCTACGAG 
CAGTATGCCC GGATGATCTT CATGGAGCTC AACGATGCGT GGGCTGAGTT TGAGAGCCAG 
GGCTCCCGGC CACTCTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024848921.1
CDS	786..4226
Translation	MELCRGQLVEFLKKVESKGPLSCDTVLKIFYQTCRAVQHMHRQKPPIIHRDLKVENLLLSNQGTIKLCDFGSATTISHYPDYSWSAQRRALVEEEITRNTTPMYRTPEIVDLYSNFPIGEKQDIWALGCILYLLCFGQHPFEDGAKLRIVNGKYAIPADDTRYSVFHSLIRSMLKVNPEERLSVTELVNQLQEIAAARNVNPKSPITELLEQNGGYGHAAPPGSTGSSLVVTEYEQPYGGFLDILRGGTERLFTNIKDTSSKVIQSVANYAKGDLDISYITSRIAVMSFPAEGVESAIKNNIEDVRLFLDSKHPGRYAVYNLSPRTYRPSKFHNRVSDCGWVARRAPSLRSLYSLCRNMHAWLRRDPGNVCVVHCMDGRAVSAVAVCSFLCFCRLFSTAEAAVYMFSMKRCPPGIWPSHKRYIEYMCDMVAEEPVTPHSKPILVKAVVMTPVPLFSKQRSGCRPFCEVYVGDERVTTTSQEYDKMRDFKIEDGKAVIPLGITVQGDVLIIIYHARSTLGGRLQAKMASMKMFQIQFHTGFVPRNAATVKFAKYDLDACDIQEKYPDLFQVSLEVEVEPRDRPGHEAPPWEGAGMRGLNPKILFSSREEQQDVLSKFGKPELPRQPGSTAQYDAEAGPADAEPPESDSPHSSVDADHFLHTLDWREDRDSSVGPEGHLAEEGPSAVAEDGCVSEPSDEEAPTPSAESGDTGDEDVPAPAAPQDDSVDLLGLHAEAGPVPAPQAPGGPPSNADLLGCLLGAPGPAPEGAVGGLLDGDAALVFSSPAPAPCTQTTAPAADPFGPLLLPPDVDTPPSSQPDLFGEFLNSDAPAAQPAPFPSTHSAPPPACSTDFLHLGDLPAEPSKMTASASHPDLLGAWDAWAEAPAPALAADGTFFSPGDQPAPSGLPAGQTKSQSQDPFADLGDLSAGLQGSPAAAAPGGFGPKAAPPPKASSSWQTSRPPAQGTPWPPQAKPPPKASVPPRPNYTSSFSVIGSREERGVRAPSFGPKPRVSENDFEDLLSNQGFSSRADRKGPRTIAEMRRQDQARDLDPLKLKLLEWTEGKERNIRALLSTLHTALWDGESRWTPVGMADLVTPAQVKKHYRRAVLVVHPDKARGQPYEQYARMIFMELNDAWAEFESQGSRPLF

Target ORF information:

RefSeq Version	XM_024993153.1
Organism	Bos taurus(cattle)
Definition	Bos taurus cyclin G associated kinase (GAK), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024993153.1

ORF Insert Sequence:

ATGGAGCTCT GCAGAGGGCA GCTGGTGGAA TTTTTAAAAA AAGTTGAATC GAAAGGTCCG 
CTCTCGTGCG ACACCGTCCT GAAGATCTTC TACCAGACGT GCCGGGCGGT GCAGCACATG 
CACAGACAGA AGCCGCCCAT CATCCACAGG GACCTCAAGG TTGAAAACTT ACTGCTTAGT 
AACCAGGGGA CCATCAAGCT GTGTGACTTC GGCAGTGCCA CGACCATCTC ACACTACCCA 
GACTACAGCT GGAGCGCCCA GCGCCGGGCC CTGGTGGAGG AGGAGATCAC CAGGAACACG 
ACCCCCATGT ACAGGACGCC GGAAATTGTG GACTTGTACT CAAACTTTCC GATTGGCGAG 
AAGCAGGACA TCTGGGCCCT GGGCTGCATC CTGTACCTGC TCTGCTTCGG GCAGCACCCC 
TTTGAGGACG GAGCGAAGCT TCGCATCGTC AACGGGAAGT ACGCAATCCC TGCCGACGAC 
ACGCGGTACT CCGTGTTCCA CAGCCTCATC CGTTCCATGC TCAAGGTCAA CCCGGAGGAG 
CGGCTGTCCG TCACCGAGCT GGTCAACCAG CTCCAGGAGA TCGCGGCCGC CCGCAACGTG 
AACCCCAAGT CACCCATCAC TGAGCTCCTG GAGCAGAATG GAGGCTATGG GCACGCGGCC 
CCTCCCGGCT CCACGGGCAG CAGTCTGGTG GTCACTGAGT ACGAGCAGCC CTATGGCGGG 
TTCCTGGACA TTCTGCGTGG GGGCACCGAG CGCCTCTTCA CCAACATCAA GGACACCTCC 
TCCAAAGTCA TCCAGTCCGT TGCCAACTAT GCGAAGGGAG ATCTGGATAT TTCTTACATC 
ACGTCTAGAA TCGCAGTCAT GTCGTTCCCG GCGGAGGGCG TGGAGTCGGC CATCAAGAAC 
AACATTGAGG ATGTACGGCT GTTCCTGGAC TCGAAGCACC CGGGCCGCTA CGCCGTCTAC 
AACCTGTCCC CGCGGACCTA CCGGCCCTCG AAGTTCCACA ACCGGGTCTC CGACTGCGGC 
TGGGTGGCCC GGCGGGCCCC GAGCCTCCGC AGCCTGTACA GCCTCTGCAG GAACATGCAC 
GCCTGGCTGC GCCGCGACCC TGGCAACGTC TGCGTGGTGC ACTGCATGGA CGGGAGGGCG 
GTGTCGGCCG TGGCCGTGTG CTCCTTCTTG TGCTTCTGCC GGCTCTTCAG CACCGCGGAG 
GCCGCTGTGT ACATGTTCAG CATGAAGCGG TGCCCACCGG GCATCTGGCC ATCCCACAAG 
AGGTACATTG AGTACATGTG TGACATGGTG GCCGAGGAGC CCGTCACGCC CCACAGCAAG 
CCCATCCTGG TGAAGGCCGT GGTCATGACG CCTGTGCCGC TCTTCAGCAA GCAGAGGAGC 
GGCTGCAGGC CCTTCTGCGA GGTCTACGTG GGGGATGAGC GCGTGACCAC CACGTCCCAG 
GAATACGACA AGATGAGAGA CTTTAAAATT GAGGATGGCA AAGCGGTCAT TCCCCTGGGC 
ATAACAGTCC AGGGGGACGT GCTCATCATC ATCTATCATG CGAGGTCCAC CCTGGGAGGA 
AGGCTGCAGG CCAAGATGGC GTCCATGAAG ATGTTTCAGA TCCAGTTTCA CACAGGGTTC 
GTGCCTCGGA ACGCAGCTAC TGTGAAGTTT GCCAAGTATG ATCTGGATGC TTGCGACATC 
CAGGAGAAGT ACCCCGACTT GTTCCAAGTG AGCCTGGAGG TGGAGGTGGA GCCCAGAGAC 
AGGCCCGGCC ACGAGGCCCC GCCCTGGGAG GGTGCAGGCA TGCGAGGGCT GAACCCCAAG 
ATCCTCTTCT CCAGCCGGGA GGAGCAGCAA GATGTCCTGT CCAAGTTTGG GAAGCCAGAG 
CTCCCCAGGC AGCCTGGGTC CACCGCACAG TACGACGCCG AGGCCGGGCC CGCGGATGCC 
GAGCCCCCCG AGTCAGACTC GCCGCACAGC AGCGTGGATG CTGACCACTT CCTGCACACG 
CTGGACTGGC GGGAGGATCG GGACTCCAGT GTGGGCCCGG AGGGTCACCT GGCCGAGGAG 
GGTCCCTCTG CTGTGGCCGA GGACGGCTGT GTGAGTGAGC CATCGGACGA GGAAGCCCCG 
ACACCCTCTG CGGAGAGCGG GGACACAGGC GATGAGGACG TGCCCGCCCC CGCCGCCCCG 
CAGGACGACA GCGTGGACCT TCTGGGGCTG CACGCGGAGG CGGGGCCTGT GCCCGCCCCC 
CAGGCCCCCG GCGGCCCCCC CAGCAATGCA GACCTGCTCG GCTGCCTGCT CGGGGCCCCG 
GGCCCCGCTC CGGAGGGTGC TGTGGGGGGC CTGCTCGACG GGGATGCGGC CCTGGTCTTC 
AGCAGCCCAG CCCCCGCGCC TTGCACCCAG ACAACTGCCC CGGCCGCTGA CCCGTTCGGC 
CCGCTCCTGC TGCCGCCCGA CGTGGACACC CCTCCTAGCT CGCAGCCTGA CCTCTTCGGG 
GAATTCCTCA ACTCAGACGC CCCGGCCGCC CAGCCCGCGC CCTTCCCCTC CACCCACAGC 
GCTCCGCCGC CAGCCTGCAG CACCGACTTC CTGCATCTTG GGGACCTGCC CGCCGAGCCC 
AGCAAGATGA CAGCCTCTGC CAGCCACCCC GACCTGCTGG GAGCCTGGGA TGCCTGGGCT 
GAGGCGCCGG CCCCCGCGCT GGCTGCAGAC GGCACCTTCT TCTCTCCTGG AGACCAGCCA 
GCCCCTTCTG GCCTTCCGGC TGGCCAGACC AAGTCTCAGA GCCAGGACCC GTTTGCAGAC 
CTTGGCGACC TCAGTGCCGG CCTCCAAGGC TCGCCTGCCG CAGCTGCTCC TGGGGGCTTT 
GGTCCCAAAG CAGCGCCCCC TCCCAAGGCC AGCAGCTCCT GGCAGACGAG TCGGCCCCCA 
GCCCAGGGCA CCCCGTGGCC CCCCCAGGCC AAGCCGCCCC CCAAAGCCAG TGTACCGCCA 
AGGCCTAACT ACACCTCCAG CTTCAGTGTG ATCGGTAGCC GGGAGGAGCG GGGGGTCCGC 
GCCCCCAGCT TTGGTCCAAA GCCGAGGGTG TCAGAGAACG ACTTTGAAGA CCTGCTCTCC 
AACCAGGGCT TCTCCTCGCG GGCTGACAGG AAGGGGCCGC GGACCATCGC TGAGATGAGG 
AGGCAGGACC AGGCCCGGGA CTTGGATCCG CTGAAGCTGA AGCTTCTGGA GTGGACGGAG 
GGCAAGGAGA GGAACATCCG CGCCCTGCTG TCCACGCTGC ACACGGCGCT GTGGGACGGC 
GAGAGCCGCT GGACGCCGGT GGGCATGGCC GACCTGGTGA CGCCCGCACA AGTGAAGAAG 
CACTACCGCC GTGCCGTGCT GGTGGTCCAC CCCGACAAGG CCCGGGGGCA GCCCTACGAG 
CAGTATGCCC GGATGATCTT CATGGAGCTC AACGATGCGT GGGCTGAGTT TGAGAGCCAG 
GGCTCCCGGC CACTCTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAK ( XM_010806359.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010804661.2 Bos taurus cyclin G associated kinase (GAK), transcript variant X6, mRNA.

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CloneID	OBa235259
Clone ID Related Accession (Same CDS sequence)	XM_010806359.3
Accession Version	XM_010806359.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3417bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	cyclin-G-associated kinase isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010806359.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCTGC TGCAGTCCGC GCTCGACTTC CTGGCGGGCC CGGGCTCCCT GGGGGGCGCG 
GCCGGCCGCG ACCAGACGGA CTTCGTGGGG CAGACGGTGG AGATGGGCGA GCTGCGGCTG 
CGGGTGCGCC GGGTCCTGGC TGAAGGAGGG TTTGCATTTG TGTATGAAGC TCAAGATCTG 
GGAAGTGGCA GAGAGTATGC ATTGAAGAGA CTGCTGTCCA ACGAAGAGGA GAAGAACAGA 
GCCATCATTC AGGAAGTGTG TTTCATGAAA AAACTTTCAG GCCACCCAAA CATTGTCCAG 
TTTTGTTCTG CAGCATCGAT AGGAAAAGAG GAGTCGGACA CTGGGCAGGC GGAGTTCCTG 
CTGCTCATGG AGCTCTGCAG AGGGCAGCTG GTGGAATTTT TAAAAAAAGT TGAATCGAAA 
GGTCCGCTCT CGTGCGACAC CGTCCTGAAG ATCTTCTACC AGACGTGCCG GGCGGTGCAG 
CACATGCACA GACAGAAGCC GCCCATCATC CACAGGGACC TCAAGGTTGA AAACTTACTG 
CTTAGTAACC AGGGGACCAT CAAGCTGTGT GACTTCGGCA GTGCCACGAC CATCTCACAC 
TACCCAGACT ACAGCTGGAG CGCCCAGCGC CGGGCCCTGG TGGAGGAGGA GATCACCAGG 
AACACGACCC CCATGTACAG GACGCCGGAA ATTGTGGACT TGTACTCAAA CTTTCCGATT 
GGCGAGAAGC AGGACATCTG GGCCCTGGGC TGCATCCTGT ACCTGCTCTG CTTCGGGCAG 
CACCCCTTTG AGGACGGAGC GAAGCTTCGC ATCGTCAACG GGAAGTACGC AATCCCTGCC 
GACGACACGC GGTACTCCGT GTTCCACAGC CTCATCCGTT CCATGCTCAA GGTCAACCCG 
GAGGAGCGGC TGTCCGTCAC CGAGCTGGTC AACCAGCTCC AGGAGATCGC GGCCGCCCGC 
AACGTGAACC CCAAGTCACC CATCACTGAG CTCCTGGAGC AGAATGGAGG CTATGGGCAC 
GCGGCCCCTC CCGGCTCCAC GGGCAGCAGT CTGGTGGTCA CTGAGTACGA GCAGCCCTAT 
GGCGGGTTCC TGGACATTCT GCGTGGGGGC ACCGAGCGCC TCTTCACCAA CATCAAGGAC 
ACCTCCTCCA AAGTCATCCA GTCCGTTGCC AACTATGCGA AGGGAGATCT GGATATTTCT 
TACATCACGT CTAGAATCGC AGTCATGTCG TTCCCGGCGG AGGGCGTGGA GTCGGCCATC 
AAGAACAACA TTGAGGATGT ACGGCTGTTC CTGGACTCGA AGCACCCGGG CCGCTACGCC 
GTCTACAACC TGTCCCCGCG GACCTACCGG CCCTCGAAGT TCCACAACCG GGTCTCCGAC 
TGCGGCTGGG TGGCCCGGCG GGCCCCGAGC CTCCGCAGCC TGTACAGCCT CTGCAGGAAC 
ATGCACGCCT GGCTGCGCCG CGACCCTGGC AACGTCTGCG TGGTGCACTG CATGGACGGG 
AGGGCGGTGT CGGCCGTGGC CGTGTGCTCC TTCTTGTGCT TCTGCCGGCT CTTCAGCACC 
GCGGAGGCCG CTGTGTACAT GTTCAGCATG AAGCGGTGCC CACCGGGCAT CTGGCCATCC 
CACAAGAGGT ACATTGAGTA CATGTGTGAC ATGGTGGCCG AGGAGCCCGT CACGCCCCAC 
AGCAAGCCCA TCCTGGTGAA GGCCGTGGTC ATGACGCCTG TGCCGCTCTT CAGCAAGCAG 
AGGAGCGGCT GCAGGCCCTT CTGCGAGGTC TACGTGGGGG ATGAGCGCGT GACCACCACG 
TCCCAGGAAT ACGACAAGAT GAGAGACTTT AAAATTGAGG ATGGCAAAGC GGTCATTCCC 
CTGGGCATAA CAGTCCAGGG GGACGTGCTC ATCATCATCT ATCATGCGAG GTCCACCCTG 
GGAGGAAGGC TGCAGGCCAA GATGGCGTCC ATGAAGATGT TTCAGATCCA GTTTCACACA 
GGGTTCGTGC CTCGGAACGC AGCTACTGTG AAGTTTGCCA AGTATGATCT GGATGCTTGC 
GACATCCAGG AGAAGTACCC CGACTTGTTC CAAGTGAGCC TGGAGGTGGA GGTGGAGCCC 
AGAGACAGGC CCGGCCACGA GGCCCCGCCC TGGGAGGGTG CAGGCATGCG AGGGCTGAAC 
CCCAAGATCC TCTTCTCCAG CCGGGAGGAG CAGCAAGATG TCCTGTCCAA GTTTGGGAAG 
CCAGAGCTCC CCAGGCAGCC TGGGTCCACC GCACAGTACG ACGCCGAGGC CGGGCCCGCG 
GATGCCGAGC CCCCCGAGTC AGACTCGCCG CACAGCAGCG TGGATGCTGA CCACTTCCTG 
CACACGCTGG ACTGGCGGGA GGATCGGGAC TCCAGTGTGG GCCCGGAGGG TCACCTGGCC 
GAGGAGGGTC CCTCTGCTGT GGCCGAGGAC GGCTGTGTGA GTGAGCCATC GGACGAGGAA 
GCCCCGACAC CCTCTGCGGA GAGCGGGGAC ACAGGCGATG AGGACGTGCC CGCCCCCGCC 
GCCCCGCAGG ACGACAGCGT GGACCTTCTG GGGCTGCACG CGGAGGCGGG GCCTGTGCCC 
GCCCCCCAGG CCCCCGGCGG CCCCCCCAGC AATGCAGACC TGCTCGGCTG CCTGCTCGGG 
GCCCCGGGCC CCGCTCCGGA GGGTGCTGTG GGGGGCCTGC TCGACGGGGA TGCGGCCCTG 
GTCTTCAGCA GCCCAGCCCC CGCGCCTTGC ACCCAGACAA CTGCCCCGGC CGCTGACCCG 
TTCGGCCCGC TCCTGCTGCC GCCCGACGTG GACACCCCTC CTAGCTCGCA GCCTGACCTC 
TTCGGGGAAT TCCTCAACTC AGACGCCCCG GCCGCCCAGC CCGCGCCCTT CCCCTCCACC 
CACAGCGCTC CGCCGCCAGC CTGCAGCACC GACTTCCTGC ATCTTGGGGA CCTGCCCGCC 
GAGCCCAGCA AGATGACAGC CTCTGCCAGC CACCCCGACC TGCTGGGAGC CTGGGATGCC 
TGGGCTGAGG CGCCGGCCCC CGCGCTGGCT GCAGACGGCA CCTTCTTCTC TCCTGGAGAC 
CAGCCAGCCC CTTCTGGCCT TCCGGCTGGC CAGACCAAGT CTCAGAGCCA GGACCCGTTT 
GCAGACCTTG GCGACCTCAG TGCCGGCCTC CAAGGGCCCA GCAACAGCAG CAGATGCCCC 
TCTGCCTTCT CTCAGGAGCT TGTTGCCAAG ACAACGCCAG GAGCTGCCAG AGCAGGCACA 
GGCACTGGCT CCTCTGCCAG CTCCATGGAG TGTGGGGGCC AGCTGGGGGC TGCCTCAGCC 
CAGGATGGTG GAACCTGCCT GCCCCGCTTG GAGTGTGGGG CCGGGGTGCA GAATTCACCA 
CCATCACTGT CCTTTCTGAG AAGCCCTCTA AGGACGTTGG TTCTTTGGAG GCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010804661.2
CDS	114..3530
Translation	MSLLQSALDFLAGPGSLGGAAGRDQTDFVGQTVEMGELRLRVRRVLAEGGFAFVYEAQDLGSGREYALKRLLSNEEEKNRAIIQEVCFMKKLSGHPNIVQFCSAASIGKEESDTGQAEFLLLMELCRGQLVEFLKKVESKGPLSCDTVLKIFYQTCRAVQHMHRQKPPIIHRDLKVENLLLSNQGTIKLCDFGSATTISHYPDYSWSAQRRALVEEEITRNTTPMYRTPEIVDLYSNFPIGEKQDIWALGCILYLLCFGQHPFEDGAKLRIVNGKYAIPADDTRYSVFHSLIRSMLKVNPEERLSVTELVNQLQEIAAARNVNPKSPITELLEQNGGYGHAAPPGSTGSSLVVTEYEQPYGGFLDILRGGTERLFTNIKDTSSKVIQSVANYAKGDLDISYITSRIAVMSFPAEGVESAIKNNIEDVRLFLDSKHPGRYAVYNLSPRTYRPSKFHNRVSDCGWVARRAPSLRSLYSLCRNMHAWLRRDPGNVCVVHCMDGRAVSAVAVCSFLCFCRLFSTAEAAVYMFSMKRCPPGIWPSHKRYIEYMCDMVAEEPVTPHSKPILVKAVVMTPVPLFSKQRSGCRPFCEVYVGDERVTTTSQEYDKMRDFKIEDGKAVIPLGITVQGDVLIIIYHARSTLGGRLQAKMASMKMFQIQFHTGFVPRNAATVKFAKYDLDACDIQEKYPDLFQVSLEVEVEPRDRPGHEAPPWEGAGMRGLNPKILFSSREEQQDVLSKFGKPELPRQPGSTAQYDAEAGPADAEPPESDSPHSSVDADHFLHTLDWREDRDSSVGPEGHLAEEGPSAVAEDGCVSEPSDEEAPTPSAESGDTGDEDVPAPAAPQDDSVDLLGLHAEAGPVPAPQAPGGPPSNADLLGCLLGAPGPAPEGAVGGLLDGDAALVFSSPAPAPCTQTTAPAADPFGPLLLPPDVDTPPSSQPDLFGEFLNSDAPAAQPAPFPSTHSAPPPACSTDFLHLGDLPAEPSKMTASASHPDLLGAWDAWAEAPAPALAADGTFFSPGDQPAPSGLPAGQTKSQSQDPFADLGDLSAGLQGPSNSSRCPSAFSQELVAKTTPGAARAGTGTGSSASSMECGGQLGAASAQDGGTCLPRLECGAGVQNSPPSLSFLRSPLRTLVLWRP

Target ORF information:

RefSeq Version	XM_010806359.3
Organism	Bos taurus(cattle)
Definition	Bos taurus cyclin G associated kinase (GAK), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010806359.3

ORF Insert Sequence:

ATGTCGCTGC TGCAGTCCGC GCTCGACTTC CTGGCGGGCC CGGGCTCCCT GGGGGGCGCG 
GCCGGCCGCG ACCAGACGGA CTTCGTGGGG CAGACGGTGG AGATGGGCGA GCTGCGGCTG 
CGGGTGCGCC GGGTCCTGGC TGAAGGAGGG TTTGCATTTG TGTATGAAGC TCAAGATCTG 
GGAAGTGGCA GAGAGTATGC ATTGAAGAGA CTGCTGTCCA ACGAAGAGGA GAAGAACAGA 
GCCATCATTC AGGAAGTGTG TTTCATGAAA AAACTTTCAG GCCACCCAAA CATTGTCCAG 
TTTTGTTCTG CAGCATCGAT AGGAAAAGAG GAGTCGGACA CTGGGCAGGC GGAGTTCCTG 
CTGCTCATGG AGCTCTGCAG AGGGCAGCTG GTGGAATTTT TAAAAAAAGT TGAATCGAAA 
GGTCCGCTCT CGTGCGACAC CGTCCTGAAG ATCTTCTACC AGACGTGCCG GGCGGTGCAG 
CACATGCACA GACAGAAGCC GCCCATCATC CACAGGGACC TCAAGGTTGA AAACTTACTG 
CTTAGTAACC AGGGGACCAT CAAGCTGTGT GACTTCGGCA GTGCCACGAC CATCTCACAC 
TACCCAGACT ACAGCTGGAG CGCCCAGCGC CGGGCCCTGG TGGAGGAGGA GATCACCAGG 
AACACGACCC CCATGTACAG GACGCCGGAA ATTGTGGACT TGTACTCAAA CTTTCCGATT 
GGCGAGAAGC AGGACATCTG GGCCCTGGGC TGCATCCTGT ACCTGCTCTG CTTCGGGCAG 
CACCCCTTTG AGGACGGAGC GAAGCTTCGC ATCGTCAACG GGAAGTACGC AATCCCTGCC 
GACGACACGC GGTACTCCGT GTTCCACAGC CTCATCCGTT CCATGCTCAA GGTCAACCCG 
GAGGAGCGGC TGTCCGTCAC CGAGCTGGTC AACCAGCTCC AGGAGATCGC GGCCGCCCGC 
AACGTGAACC CCAAGTCACC CATCACTGAG CTCCTGGAGC AGAATGGAGG CTATGGGCAC 
GCGGCCCCTC CCGGCTCCAC GGGCAGCAGT CTGGTGGTCA CTGAGTACGA GCAGCCCTAT 
GGCGGGTTCC TGGACATTCT GCGTGGGGGC ACCGAGCGCC TCTTCACCAA CATCAAGGAC 
ACCTCCTCCA AAGTCATCCA GTCCGTTGCC AACTATGCGA AGGGAGATCT GGATATTTCT 
TACATCACGT CTAGAATCGC AGTCATGTCG TTCCCGGCGG AGGGCGTGGA GTCGGCCATC 
AAGAACAACA TTGAGGATGT ACGGCTGTTC CTGGACTCGA AGCACCCGGG CCGCTACGCC 
GTCTACAACC TGTCCCCGCG GACCTACCGG CCCTCGAAGT TCCACAACCG GGTCTCCGAC 
TGCGGCTGGG TGGCCCGGCG GGCCCCGAGC CTCCGCAGCC TGTACAGCCT CTGCAGGAAC 
ATGCACGCCT GGCTGCGCCG CGACCCTGGC AACGTCTGCG TGGTGCACTG CATGGACGGG 
AGGGCGGTGT CGGCCGTGGC CGTGTGCTCC TTCTTGTGCT TCTGCCGGCT CTTCAGCACC 
GCGGAGGCCG CTGTGTACAT GTTCAGCATG AAGCGGTGCC CACCGGGCAT CTGGCCATCC 
CACAAGAGGT ACATTGAGTA CATGTGTGAC ATGGTGGCCG AGGAGCCCGT CACGCCCCAC 
AGCAAGCCCA TCCTGGTGAA GGCCGTGGTC ATGACGCCTG TGCCGCTCTT CAGCAAGCAG 
AGGAGCGGCT GCAGGCCCTT CTGCGAGGTC TACGTGGGGG ATGAGCGCGT GACCACCACG 
TCCCAGGAAT ACGACAAGAT GAGAGACTTT AAAATTGAGG ATGGCAAAGC GGTCATTCCC 
CTGGGCATAA CAGTCCAGGG GGACGTGCTC ATCATCATCT ATCATGCGAG GTCCACCCTG 
GGAGGAAGGC TGCAGGCCAA GATGGCGTCC ATGAAGATGT TTCAGATCCA GTTTCACACA 
GGGTTCGTGC CTCGGAACGC AGCTACTGTG AAGTTTGCCA AGTATGATCT GGATGCTTGC 
GACATCCAGG AGAAGTACCC CGACTTGTTC CAAGTGAGCC TGGAGGTGGA GGTGGAGCCC 
AGAGACAGGC CCGGCCACGA GGCCCCGCCC TGGGAGGGTG CAGGCATGCG AGGGCTGAAC 
CCCAAGATCC TCTTCTCCAG CCGGGAGGAG CAGCAAGATG TCCTGTCCAA GTTTGGGAAG 
CCAGAGCTCC CCAGGCAGCC TGGGTCCACC GCACAGTACG ACGCCGAGGC CGGGCCCGCG 
GATGCCGAGC CCCCCGAGTC AGACTCGCCG CACAGCAGCG TGGATGCTGA CCACTTCCTG 
CACACGCTGG ACTGGCGGGA GGATCGGGAC TCCAGTGTGG GCCCGGAGGG TCACCTGGCC 
GAGGAGGGTC CCTCTGCTGT GGCCGAGGAC GGCTGTGTGA GTGAGCCATC GGACGAGGAA 
GCCCCGACAC CCTCTGCGGA GAGCGGGGAC ACAGGCGATG AGGACGTGCC CGCCCCCGCC 
GCCCCGCAGG ACGACAGCGT GGACCTTCTG GGGCTGCACG CGGAGGCGGG GCCTGTGCCC 
GCCCCCCAGG CCCCCGGCGG CCCCCCCAGC AATGCAGACC TGCTCGGCTG CCTGCTCGGG 
GCCCCGGGCC CCGCTCCGGA GGGTGCTGTG GGGGGCCTGC TCGACGGGGA TGCGGCCCTG 
GTCTTCAGCA GCCCAGCCCC CGCGCCTTGC ACCCAGACAA CTGCCCCGGC CGCTGACCCG 
TTCGGCCCGC TCCTGCTGCC GCCCGACGTG GACACCCCTC CTAGCTCGCA GCCTGACCTC 
TTCGGGGAAT TCCTCAACTC AGACGCCCCG GCCGCCCAGC CCGCGCCCTT CCCCTCCACC 
CACAGCGCTC CGCCGCCAGC CTGCAGCACC GACTTCCTGC ATCTTGGGGA CCTGCCCGCC 
GAGCCCAGCA AGATGACAGC CTCTGCCAGC CACCCCGACC TGCTGGGAGC CTGGGATGCC 
TGGGCTGAGG CGCCGGCCCC CGCGCTGGCT GCAGACGGCA CCTTCTTCTC TCCTGGAGAC 
CAGCCAGCCC CTTCTGGCCT TCCGGCTGGC CAGACCAAGT CTCAGAGCCA GGACCCGTTT 
GCAGACCTTG GCGACCTCAG TGCCGGCCTC CAAGGGCCCA GCAACAGCAG CAGATGCCCC 
TCTGCCTTCT CTCAGGAGCT TGTTGCCAAG ACAACGCCAG GAGCTGCCAG AGCAGGCACA 
GGCACTGGCT CCTCTGCCAG CTCCATGGAG TGTGGGGGCC AGCTGGGGGC TGCCTCAGCC 
CAGGATGGTG GAACCTGCCT GCCCCGCTTG GAGTGTGGGG CCGGGGTGCA GAATTCACCA 
CCATCACTGT CCTTTCTGAG AAGCCCTCTA AGGACGTTGG TTCTTTGGAG GCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAK ( NM_001046084.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001039549.1 Bos taurus cyclin G associated kinase (GAK), mRNA.

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CloneID	OBa40735
Clone ID Related Accession (Same CDS sequence)	NM_001046084.2
Accession Version	NM_001046084.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3807bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-13
Organism	Bos taurus(cattle)
Product	cyclin-G-associated kinase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC105343.1. On Aug 31, 2012 this sequence version replaced NM_001046084.1. ##Evidence-Data-START## Transcript exon combination :: BC105343.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END##

ATGTCGCTGC TGCAGTCCGC GCTCGACTTC CTGGCGGGCC CGGGCTCCCT GGGGGGCGCG 
GCCGGCCGCG ACCAGACGGA CTTCGTGGGG CAGACGGTGG AGATGGGCGA GCTGCGGCTG 
CGGGTGCGCC GGGTCCTGGC TGAAGGAGGG TTTGCATTTG TGTATGAAGC TCAAGATCTG 
GGAAGTGGCA GAGAGTATGC ATTGAAGAGA CTGCTGTCCA ACGAAGAGGA GAAGAACAGA 
GCCATCATTC AGGAAGTGTG TTTCATGAAA AAACTTTCAG GCCACCCAAA CATTGTCCAG 
TTTTGTTCTG CAGCATCGAT AGGAAAAGAG GAGTCGGACA CTGGGCAGGC GGAGTTCCTG 
CTGCTCATGG AGCTCTGCAG AGGGCAGCTG GTGGAATTTT TAAAAAAAGT TGAATCGAAA 
GGTCCGCTCT CGTGCGACAC CGTCCTGAAG ATCTTCTACC AGACGTGCCG GGCGGTGCAG 
CACATGCACA GACAGAAGCC GCCCATCATC CACAGGGACC TCAAGGTTGA AAACTTACTG 
CTTAGTAACC AGGGGACCAT CAAGCTGTGT GACTTCGGCA GTGCCACGAC CATCTCACAC 
TACCCAGACT ACAGCTGGAG CGCCCAGCGC CGGGCCCTGG TGGAGGAGGA GATCACCAGG 
AACACGACCC CCATGTACAG GACGCCGGAA ATTGTGGACT TGTACTCAAA CTTTCCGATT 
GGCGAGAAGC AGGACATCTG GGCCCTGGGC TGCATCCTGT ACCTGCTCTG CTTCGGGCAG 
CACCCCTTTG AGGACGGAGC GAAGCTTCGC ATCGTCAACG GGAAGTACGC AATCCCTGCC 
GACGACACGC GGTACTCCGT GTTCCACAGC CTCATCCGTT CCATGCTCAA GGTCAACCCG 
GAGGAGCGGC TGTCCGTCAC CGAGCTGGTC AACCAGCTCC AGGAGATCGC GGCCGCCCGC 
AACGTGAACC CCAAGTCACC CATCACTGAG CTCCTGGAGC AGAATGGAGG CTATGGGCAC 
GCGGCCCCTC CCGGCTCCAC GGGCAGCAGT CTGGTGGTCA CTGAGTACGA GCAGCCCTAT 
GGCGGGTTCC TGGACATTCT GCGTGGGGGC ACCGAGCGCC TCTTCACCAA CATCAAGGAC 
ACCTCCTCCA AAGTCATCCA GTCCGTTGCC AACTATGCGA AGGGAGATCT GGATATTTCT 
TACATCACGT CTAGAATCGC AGTCATGTCG TTCCCGGCGG AGGGCGTGGA GTCGGCCATC 
AAGAACAACA TTGAGGATGT ACGGCTGTTC CTGGACTCGA AGCACCCGGG CCGCTACGCC 
GTCTACAACC TGTCCCCGCG GACCTACCGG CCCTCGAAGT TCCACAACCG GGTCTCCGAC 
TGCGGCTGGG TGGCCCGGCG GGCCCCGAGC CTCCGCAGCC TGTACAGCCT CTGCAGGAAC 
ATGCACGCCT GGCTGCGCCG CGACCCTGGC AACGTCTGCG TGGTGCACTG CATGGACGGG 
AGGGCGGTGT CGGCCGTGGC CGTGTGCTCC TTCTTGTGCT TCTGCCGGCT CTTCAGCACC 
GCGGAGGCCG CTGTGTACAT GTTCAGCATG AAGCGGTGCC CACCGGGCAT CTGGCCATCC 
CACAAGAGGT ACATTGAGTA CATGTGTGAC ATGGTGGCCG AGGAGCCCGT CACGCCCCAC 
AGCAAGCCCA TCCTGGTGAA GGCCGTGGTC ATGACGCCTG TGCCGCTCTT CAGCAAGCAG 
AGGAGCGGCT GCAGGCCCTT CTGCGAGGTC TACGTGGGGG ATGAGCGCGT GACCACCACG 
TCCCAGGAAT ACGACAAGAT GAGAGACTTT AAAATTGAGG ATGGCAAAGC GGTCATTCCC 
CTGGGCATAA CAGTCCAGGG GGACGTGCTC ATCATCATCT ATCATGCGAG GTCCACCCTG 
GGAGGAAGGC TGCAGGCCAA GATGGCGTCC ATGAAGATGT TTCAGATCCA GTTTCACACA 
GGGTTCGTGC CTCGGAACGC AGCTACTGTG AAGTTTGCCA AGTATGATCT GGATGCTTGC 
GACATCCAGG AGAAGTACCC CGACTTGTTC CAAGTGAGCC TGGAGGTGGA GGTGGAGCCC 
AGAGACAGGC CCGGCCACGA GGCCCCGCCC TGGGAGGGTG CAGGCATGCG AGGGCTGAAC 
CCCAAGATCC TCTTCTCCAG CCGGGAGGAG CAGCAAGATG TCCTGTCCAA GTTTGGGAAG 
CCAGAGCTCC CCAGGCAGCC TGGGTCCACC GCACAGTACG ACGCCGAGGC CGGGCCCGCG 
GATGCCGAGC CCCCCGAGTC AGACTCGCCG CACAGCAGCG TGGATGCTGA CCACTTCCTG 
CACACGCTGG ACTGGCGGGA GGATCGGGAC TCCAGTGTGG GCCCGGAGGG TCACCTGGCC 
GAGGAGGGTC CCTCTGCTGT GGCCGAGGAC GGCTGTGTGA GTGAGCCATC GGACGAGGAA 
GCCCCGACAC CCTCTGCGGA GAGCGGGGAC ACAGGCGATG AGGACGTGCC CGCCCCCGCC 
GCCCCGCAGG ACGACAGCGT GGACCTTCTG GGGCTGCACG CGGAGGCGGG GCCTGTGCCC 
GCCCCCCAGG CCCCCGGCGG CCCCCCCAGC AATGCAGACC TGCTCGGCTG CCTGCTCGGG 
GCCCCGGGCC CCGCTCCGGA GGGTGCTGTG GGGGGCCTGC TCGACGGGGA TGCGGCCCTG 
GTCTTCAGCA GCCCAGCCCC CGCGCCTTGC ACCCAGACAA CTGCCCCGGC CGCTGACCCG 
TTCGGCCCGC TCCTGCTGCC GCCCGACGTG GACACCCCTC CTAGCTCGCA GCCTGACCTC 
TTCGGGGAAT TCCTCAACTC AGACGCCCCG GCCGCCCAGC CCGCGCCCTT CCCCTCCACG 
CACAGCGCTC CGCCGCCAGC CTGCAGCACC GACTTCCTGC ATCTTGGGGA CCTGCCCGCC 
GAGCCCAGCA AGATGACAGC CTCTGCCAGC CACCCCGACC TGCTGGGAGC CTGGGATGCC 
TGGGCTGAGG CGCCGGCCCC CGCGCTGGCT GCAGACGGCA CCTTCTTCTC TCCTGGAGAC 
CAGCCAGCCC CTTCTGGCCT TCCGGCTGGC CAGACCAAGT CTCAGAGCCA GGACCCGTTT 
GCAGACCTTG GCGACCTCAG TGCCGGCCTC CAAGGCTCGC CTGCCGCAGC TGCTCCTGGG 
GGCTTTGGTC CCAAAGCAGC GCCCCCTCCC AAGGCCAGCA GCTCCTGGCA GACGAGTCGG 
CCCCCAGCCC AGGGCACCCC GTGGCCCCCC CAGGCCAAGC CGCCCCCCAA AGCCAGTGTA 
CCGCCAAGGC CTAACTACAC CTCCAGCTTC AGTGTGATCG GTAGCCGGGA GGAGCGGGGG 
GTCCGCGCCC CCAGCTTTGG TCCAAAGCCG AGGGTGTCAG AGAACGACTT TGAAGACCTG 
CTCTCCAACC AGGGCTTCTC CTCGCGGGCT GACAGGAAGG GGCCGCGGAC CATCGCTGAG 
ATGAGGAGGC AGGACCAGGC CCGGGACTCG GATCCGCTGA AGCTGAAGCT TCTGGAGTGG 
ACGGAGGGCA AGGAGAGGAA CATCCGCGCC CTGCTGTCCA CGCTGCACAC GGCGCTGTGG 
GATGGCGAGA GCCGCTGGAC GCCGGTGGGC ATGGCCGACC TGGTGACGCC CGCACAAGTG 
AAGAAGCACT ACCGCCGTGC CGTGCTGGTG GTCCACCCCG ACAAGGCCCG GGGGCAGCCC 
TACGAGCAGT ATGCCCGGAT GATCTTCATG GAGCTCAACG ATGCGTGGGC TGAGTTTGAG 
AGCCAGGGCT CCCGGCCACT CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001039549.1
CDS	118..3924
Translation	MSLLQSALDFLAGPGSLGGAAGRDQTDFVGQTVEMGELRLRVRRVLAEGGFAFVYEAQDLGSGREYALKRLLSNEEEKNRAIIQEVCFMKKLSGHPNIVQFCSAASIGKEESDTGQAEFLLLMELCRGQLVEFLKKVESKGPLSCDTVLKIFYQTCRAVQHMHRQKPPIIHRDLKVENLLLSNQGTIKLCDFGSATTISHYPDYSWSAQRRALVEEEITRNTTPMYRTPEIVDLYSNFPIGEKQDIWALGCILYLLCFGQHPFEDGAKLRIVNGKYAIPADDTRYSVFHSLIRSMLKVNPEERLSVTELVNQLQEIAAARNVNPKSPITELLEQNGGYGHAAPPGSTGSSLVVTEYEQPYGGFLDILRGGTERLFTNIKDTSSKVIQSVANYAKGDLDISYITSRIAVMSFPAEGVESAIKNNIEDVRLFLDSKHPGRYAVYNLSPRTYRPSKFHNRVSDCGWVARRAPSLRSLYSLCRNMHAWLRRDPGNVCVVHCMDGRAVSAVAVCSFLCFCRLFSTAEAAVYMFSMKRCPPGIWPSHKRYIEYMCDMVAEEPVTPHSKPILVKAVVMTPVPLFSKQRSGCRPFCEVYVGDERVTTTSQEYDKMRDFKIEDGKAVIPLGITVQGDVLIIIYHARSTLGGRLQAKMASMKMFQIQFHTGFVPRNAATVKFAKYDLDACDIQEKYPDLFQVSLEVEVEPRDRPGHEAPPWEGAGMRGLNPKILFSSREEQQDVLSKFGKPELPRQPGSTAQYDAEAGPADAEPPESDSPHSSVDADHFLHTLDWREDRDSSVGPEGHLAEEGPSAVAEDGCVSEPSDEEAPTPSAESGDTGDEDVPAPAAPQDDSVDLLGLHAEAGPVPAPQAPGGPPSNADLLGCLLGAPGPAPEGAVGGLLDGDAALVFSSPAPAPCTQTTAPAADPFGPLLLPPDVDTPPSSQPDLFGEFLNSDAPAAQPAPFPSTHSAPPPACSTDFLHLGDLPAEPSKMTASASHPDLLGAWDAWAEAPAPALAADGTFFSPGDQPAPSGLPAGQTKSQSQDPFADLGDLSAGLQGSPAAAAPGGFGPKAAPPPKASSSWQTSRPPAQGTPWPPQAKPPPKASVPPRPNYTSSFSVIGSREERGVRAPSFGPKPRVSENDFEDLLSNQGFSSRADRKGPRTIAEMRRQDQARDSDPLKLKLLEWTEGKERNIRALLSTLHTALWDGESRWTPVGMADLVTPAQVKKHYRRAVLVVHPDKARGQPYEQYARMIFMELNDAWAEFESQGSRPLF

Target ORF information:

RefSeq Version	NM_001046084.2
Organism	Bos taurus(cattle)
Definition	Bos taurus cyclin G associated kinase (GAK), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001046084.2

ORF Insert Sequence:

ATGTCGCTGC TGCAGTCCGC GCTCGACTTC CTGGCGGGCC CGGGCTCCCT GGGGGGCGCG 
GCCGGCCGCG ACCAGACGGA CTTCGTGGGG CAGACGGTGG AGATGGGCGA GCTGCGGCTG 
CGGGTGCGCC GGGTCCTGGC TGAAGGAGGG TTTGCATTTG TGTATGAAGC TCAAGATCTG 
GGAAGTGGCA GAGAGTATGC ATTGAAGAGA CTGCTGTCCA ACGAAGAGGA GAAGAACAGA 
GCCATCATTC AGGAAGTGTG TTTCATGAAA AAACTTTCAG GCCACCCAAA CATTGTCCAG 
TTTTGTTCTG CAGCATCGAT AGGAAAAGAG GAGTCGGACA CTGGGCAGGC GGAGTTCCTG 
CTGCTCATGG AGCTCTGCAG AGGGCAGCTG GTGGAATTTT TAAAAAAAGT TGAATCGAAA 
GGTCCGCTCT CGTGCGACAC CGTCCTGAAG ATCTTCTACC AGACGTGCCG GGCGGTGCAG 
CACATGCACA GACAGAAGCC GCCCATCATC CACAGGGACC TCAAGGTTGA AAACTTACTG 
CTTAGTAACC AGGGGACCAT CAAGCTGTGT GACTTCGGCA GTGCCACGAC CATCTCACAC 
TACCCAGACT ACAGCTGGAG CGCCCAGCGC CGGGCCCTGG TGGAGGAGGA GATCACCAGG 
AACACGACCC CCATGTACAG GACGCCGGAA ATTGTGGACT TGTACTCAAA CTTTCCGATT 
GGCGAGAAGC AGGACATCTG GGCCCTGGGC TGCATCCTGT ACCTGCTCTG CTTCGGGCAG 
CACCCCTTTG AGGACGGAGC GAAGCTTCGC ATCGTCAACG GGAAGTACGC AATCCCTGCC 
GACGACACGC GGTACTCCGT GTTCCACAGC CTCATCCGTT CCATGCTCAA GGTCAACCCG 
GAGGAGCGGC TGTCCGTCAC CGAGCTGGTC AACCAGCTCC AGGAGATCGC GGCCGCCCGC 
AACGTGAACC CCAAGTCACC CATCACTGAG CTCCTGGAGC AGAATGGAGG CTATGGGCAC 
GCGGCCCCTC CCGGCTCCAC GGGCAGCAGT CTGGTGGTCA CTGAGTACGA GCAGCCCTAT 
GGCGGGTTCC TGGACATTCT GCGTGGGGGC ACCGAGCGCC TCTTCACCAA CATCAAGGAC 
ACCTCCTCCA AAGTCATCCA GTCCGTTGCC AACTATGCGA AGGGAGATCT GGATATTTCT 
TACATCACGT CTAGAATCGC AGTCATGTCG TTCCCGGCGG AGGGCGTGGA GTCGGCCATC 
AAGAACAACA TTGAGGATGT ACGGCTGTTC CTGGACTCGA AGCACCCGGG CCGCTACGCC 
GTCTACAACC TGTCCCCGCG GACCTACCGG CCCTCGAAGT TCCACAACCG GGTCTCCGAC 
TGCGGCTGGG TGGCCCGGCG GGCCCCGAGC CTCCGCAGCC TGTACAGCCT CTGCAGGAAC 
ATGCACGCCT GGCTGCGCCG CGACCCTGGC AACGTCTGCG TGGTGCACTG CATGGACGGG 
AGGGCGGTGT CGGCCGTGGC CGTGTGCTCC TTCTTGTGCT TCTGCCGGCT CTTCAGCACC 
GCGGAGGCCG CTGTGTACAT GTTCAGCATG AAGCGGTGCC CACCGGGCAT CTGGCCATCC 
CACAAGAGGT ACATTGAGTA CATGTGTGAC ATGGTGGCCG AGGAGCCCGT CACGCCCCAC 
AGCAAGCCCA TCCTGGTGAA GGCCGTGGTC ATGACGCCTG TGCCGCTCTT CAGCAAGCAG 
AGGAGCGGCT GCAGGCCCTT CTGCGAGGTC TACGTGGGGG ATGAGCGCGT GACCACCACG 
TCCCAGGAAT ACGACAAGAT GAGAGACTTT AAAATTGAGG ATGGCAAAGC GGTCATTCCC 
CTGGGCATAA CAGTCCAGGG GGACGTGCTC ATCATCATCT ATCATGCGAG GTCCACCCTG 
GGAGGAAGGC TGCAGGCCAA GATGGCGTCC ATGAAGATGT TTCAGATCCA GTTTCACACA 
GGGTTCGTGC CTCGGAACGC AGCTACTGTG AAGTTTGCCA AGTATGATCT GGATGCTTGC 
GACATCCAGG AGAAGTACCC CGACTTGTTC CAAGTGAGCC TGGAGGTGGA GGTGGAGCCC 
AGAGACAGGC CCGGCCACGA GGCCCCGCCC TGGGAGGGTG CAGGCATGCG AGGGCTGAAC 
CCCAAGATCC TCTTCTCCAG CCGGGAGGAG CAGCAAGATG TCCTGTCCAA GTTTGGGAAG 
CCAGAGCTCC CCAGGCAGCC TGGGTCCACC GCACAGTACG ACGCCGAGGC CGGGCCCGCG 
GATGCCGAGC CCCCCGAGTC AGACTCGCCG CACAGCAGCG TGGATGCTGA CCACTTCCTG 
CACACGCTGG ACTGGCGGGA GGATCGGGAC TCCAGTGTGG GCCCGGAGGG TCACCTGGCC 
GAGGAGGGTC CCTCTGCTGT GGCCGAGGAC GGCTGTGTGA GTGAGCCATC GGACGAGGAA 
GCCCCGACAC CCTCTGCGGA GAGCGGGGAC ACAGGCGATG AGGACGTGCC CGCCCCCGCC 
GCCCCGCAGG ACGACAGCGT GGACCTTCTG GGGCTGCACG CGGAGGCGGG GCCTGTGCCC 
GCCCCCCAGG CCCCCGGCGG CCCCCCCAGC AATGCAGACC TGCTCGGCTG CCTGCTCGGG 
GCCCCGGGCC CCGCTCCGGA GGGTGCTGTG GGGGGCCTGC TCGACGGGGA TGCGGCCCTG 
GTCTTCAGCA GCCCAGCCCC CGCGCCTTGC ACCCAGACAA CTGCCCCGGC CGCTGACCCG 
TTCGGCCCGC TCCTGCTGCC GCCCGACGTG GACACCCCTC CTAGCTCGCA GCCTGACCTC 
TTCGGGGAAT TCCTCAACTC AGACGCCCCG GCCGCCCAGC CCGCGCCCTT CCCCTCCACG 
CACAGCGCTC CGCCGCCAGC CTGCAGCACC GACTTCCTGC ATCTTGGGGA CCTGCCCGCC 
GAGCCCAGCA AGATGACAGC CTCTGCCAGC CACCCCGACC TGCTGGGAGC CTGGGATGCC 
TGGGCTGAGG CGCCGGCCCC CGCGCTGGCT GCAGACGGCA CCTTCTTCTC TCCTGGAGAC 
CAGCCAGCCC CTTCTGGCCT TCCGGCTGGC CAGACCAAGT CTCAGAGCCA GGACCCGTTT 
GCAGACCTTG GCGACCTCAG TGCCGGCCTC CAAGGCTCGC CTGCCGCAGC TGCTCCTGGG 
GGCTTTGGTC CCAAAGCAGC GCCCCCTCCC AAGGCCAGCA GCTCCTGGCA GACGAGTCGG 
CCCCCAGCCC AGGGCACCCC GTGGCCCCCC CAGGCCAAGC CGCCCCCCAA AGCCAGTGTA 
CCGCCAAGGC CTAACTACAC CTCCAGCTTC AGTGTGATCG GTAGCCGGGA GGAGCGGGGG 
GTCCGCGCCC CCAGCTTTGG TCCAAAGCCG AGGGTGTCAG AGAACGACTT TGAAGACCTG 
CTCTCCAACC AGGGCTTCTC CTCGCGGGCT GACAGGAAGG GGCCGCGGAC CATCGCTGAG 
ATGAGGAGGC AGGACCAGGC CCGGGACTCG GATCCGCTGA AGCTGAAGCT TCTGGAGTGG 
ACGGAGGGCA AGGAGAGGAA CATCCGCGCC CTGCTGTCCA CGCTGCACAC GGCGCTGTGG 
GATGGCGAGA GCCGCTGGAC GCCGGTGGGC ATGGCCGACC TGGTGACGCC CGCACAAGTG 
AAGAAGCACT ACCGCCGTGC CGTGCTGGTG GTCCACCCCG ACAAGGCCCG GGGGCAGCCC 
TACGAGCAGT ATGCCCGGAT GATCTTCATG GAGCTCAACG ATGCGTGGGC TGAGTTTGAG 
AGCCAGGGCT CCCGGCCACT CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAK ( XM_010806358.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010804660.1 Bos taurus cyclin G associated kinase (GAK), transcript variant X1, mRNA.

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CloneID	OBa20662
Clone ID Related Accession (Same CDS sequence)	XM_010806358.2
Accession Version	XM_010806358.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3543bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	cyclin-G-associated kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103986.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010806358.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAAAC TTTCAGGCCA CCCAAACATT GTCCAGTTTT GTTCTGCAGC ATCGATAGGA 
AAAGAGGAGT CGGACACTGG GCAGGCGGAG TTCCTGCTGC TCATGGAGCT CTGCAGAGGG 
CAGCTGGTGG AATTTTTAAA AAAAGTTGAA TCGAAAGGTC CGCTCTCGTG CGACACCGTC 
CTGAAGATCT TCTACCAGAC GTGCCGGGCG GTGCAGCACA TGCACAGACA GAAGCCGCCC 
ATCATCCACA GGGACCTCAA GGTTGAAAAC TTACTGCTTA GTAACCAGGG GACCATCAAG 
CTGTGCGACT TCGGCAGTGC CACGACCATC TCACACTACC CAGACTACAG CTGGAGCGCC 
CAGCGCCGGG CCCTGGTGGA GGAGGAGATC ACCAGGAACA CGACCCCCAT GTACAGGACG 
CCGGAAATTG TGGACTTGTA CTCAAACTTT CCGATTGGCG AGAAGCAGGA CATCTGGGCC 
CTGGGCTGCA TCCTGTACCT GCTCTGCTTC GGGCAGCACC CCTTTGAGGA CGGAGCGAAG 
CTTCGCATCG TCAACGGGAA GTACGCAATC CCTGCCGACG ACACGCGGTA CTCCGTGTTC 
CACAGCCTCA TCCGTTCCAT GCTCAAGGTC AACCCGGAGG AGCGGCTGTC CGTCACCGAG 
CTGGTCAACC AGCTCCAGGA GATCGCGGCC GCCCGCAACG TGAACCCCAA GTCACCCATC 
ACTGAGCTCC TGGAGCAGAA TGGAGGCTAT GGGCACGCGG CCCCTCCCGG CTCCACGGGC 
AGCAGTCTGG TGGTCACTGA GTACGAGCAG CCCTATGGCG GGTTCCTGGA CATTCTGCGT 
GGGGGCACCG AGCGCCTCTT CACCAACATC AAGGACACCT CCTCCAAAGT CATCCAGTCC 
GTTGCCAACT ATGCGAAGGG AGATCTGGAT ATTTCTTACA TCACGTCTAG AATCGCAGTC 
ATGTCGTTCC CGGCGGAGGG CGTGGAGTCG GCCATCAAGA ACAACATTGA GGATGTACGG 
CTGTTCCTGG ACTCGAAGCA CCCGGGCCGC TACGCCGTCT ACAACCTGTC CCCGCGGACC 
TACCGGCCCT CGAAGTTCCA CAACCGGGTC TCCGACTGCG GCTGGGTGGC CCGGCGGGCC 
CCGAGCCTCC GCAGCCTGTA CAGCCTCTGC AGGAACATGC ACGCCTGGCT GCGCCGCGAC 
CCTGGCAACG TCTGCGTGGT GCACTGCATG GACGGGAGGG CGGTGTCGGC CGTGGCCGTG 
TGCTCCTTCT TGTGCTTCTG CCGGCTCTTC AGCACCGCGG AGGCCGCTGT GTACATGTTC 
AGCATGAAGC GGTGCCCACC GGGCATCTGG CCATCCCACA AGAGGTACAT TGAGTACATG 
TGTGACATGG TGGCCGAGGA GCCCGTCACG CCCCACAGCA AGCCCATCCT GGTGAAGGCC 
GTGGTCATGA CGCCTGTGCC GCTCTTCAGC AAGCAGAGGA GCGGCTGCAG GCCCTTCTGC 
GAGGTCTACG TGGGGGATGA GCGCGTGACC ACCACGTCCC AGGAATACGA CAAGATGAGA 
GACTTTAAAA TTGAGGATGG CAAAGCGGTC ATTCCCCTGG GCATAACAGT CCAGGGGGAC 
GTGCTCATCA TCATCTATCA TGCGAGGTCC ACCCTGGGAG GAAGGCTGCA GGCCAAGATG 
GCGTCCATGA AGATGTTTCA GATCCAGTTT CACACAGGGT TCGTGCCTCG GAACGCAGCT 
ACTGTGAAGT TTGCCAAGTA TGATCTGGAT GCTTGCGACA TCCAGGAGAA GTACCCCGAC 
TTGTTCCAAG TGAGCCTGGA GGTGGAGGTG GAGCCCAGAG ACAGGCCCGG CCACGAGGCC 
CCGCCCTGGG AGGGTGCAGG CATGCGAGGG CTGAACCCCA AGATCCTCTT CTCCAGCCGG 
GAGGAGCAGC AAGATGTCCT GTCCAAGTTT GGGAAGCCAG AGCTCCCCAG GCAGCCTGGG 
TCCACCGCAC AGTACGACGC CGAGGCCGGG CCCGCGGATG CCGAGCCCCC CGAGTCAGAC 
TCGCCGCACA GCAGCGTGGA TGCTGACCAC TTCCTGCACA CGCTGGACTG GCGGGAGGAT 
CGGGACTCCA GTGTGGGCCC GGAGGGTCAC CTGGCCGAGG AGGGTCCCTC TGCTGTGGCC 
GAGGACGGCT GTGTGAGTGA GCCATCGGAC GAGGAAGCCC CGACACCCTC TGCGGAGAGC 
GGGGACACAG GCGATGAGGA CGTGCCCGCC CCCGCCGCCC CGCAGGACGA CAGCGTGGAC 
CTTCTGGGGC TGCACGCGGA GGCGGGGCCT GTGCCCGCCC CCCAGGCCCC CGGCGGCCCC 
CCCAGCAATG CAGACCTGCT CGGCTGCCTG CTCGGGGCCC CGGGCCCCGC TCCGGAGGGT 
GCTGTGGGGG GCCTGCTCGA CGGGGATGCG GCCCTGCTCT TCAGCAGCCC AGCCCCCGCG 
CCTTGCACCC AGACAACTGC CCCGGCCGCT GACCCGTTCG GCCCGCTCCT GCTGCCGCCC 
GACGTGGACA CCCCTCCTAG CTCGCAGCCT GACCTCTTCG GGGAATTCCT CAACTCAGAC 
GCCCCGGCCG CCCAGCCCGC GCCCTTCCCC TCCACCCACA GCGCTCCGCC GCCAGCCTGC 
AGCACCGACT TCCTGCATCT TGGGGACCTG CCCGCCGAGC CCAGCAAGAT GACAGCCTCT 
GCCAGCCACC CCGACCTGCT GGGAGCCTGG GATGCCTGGG CTGAGGCGCC GGCCCCCGCG 
CTGGCTGCAG ACGGCACCTT CTTCTCTCCT GGAGACCAGC CAGCCCCTTC TGGCCTTCCG 
GCTGGCCAGA CCAAGTCTCA GAGCCAGGAC CCGTTTGCAG ACCTTGGCGA CCTCAGTGCC 
GGCCTCCAAG GCTCGCCTGC CGCAGCTGCT CCTGGGGGCT TTGGTCCCAA AGCAGCGCCC 
CCTCCCAAGG CCAGCAGCTC CTGGCAGACG AGTCGGCCCC CAGCCCAGGG CACCCCGTGG 
CCCCCCCAGG CCAAGCCGCC CCCCAAAGCC AGTGTACCGC CAAGGCCTAA CTACACCTCC 
AGCTTCAGTG TGATCGGTAG CCGGGAGGAG CGGGGGGTCC GCGCCCCCAG CTTTGGTCCA 
AAGCCGAGGG TGTCAGAAAA CGACTTTGAA GACCTGCTCT CCAACCAGGG CTTCTCCTCG 
CGGGCTGACA GGAAGGGGCC GCGGACCATC GCTGAGATGA GGAGGCAGGA CCAGGCCCGG 
GACTTGGATC CGCTGAAGCT GAAGCTTCTG GAGTGGACGG AGGGCAAGGA GAGGAACATC 
CGCGCCCTGC TGTCCACGCT GCACACGGCG CTGTGGGACG GCGAGAGCCG CTGGACGCCG 
GTGGGCATGG CCGACCTGGT GACGCCCGCA CAAGTGAAGA AGCACTACCG CCGTGCCGTG 
CTGGTGGTCC ACCCCGACAA GGCCCGGGGG CAGCCCTACG AGCAGTATGC CCGGATGATC 
TTCATGGAGC TCAACGATGC GTGGGCTGAG TTTGAGAGCC AGGGCTCCCG GCCACTCTTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010804660.1
CDS	290..3832
Translation	MKKLSGHPNIVQFCSAASIGKEESDTGQAEFLLLMELCRGQLVEFLKKVESKGPLSCDTVLKIFYQTCRAVQHMHRQKPPIIHRDLKVENLLLSNQGTIKLCDFGSATTISHYPDYSWSAQRRALVEEEITRNTTPMYRTPEIVDLYSNFPIGEKQDIWALGCILYLLCFGQHPFEDGAKLRIVNGKYAIPADDTRYSVFHSLIRSMLKVNPEERLSVTELVNQLQEIAAARNVNPKSPITELLEQNGGYGHAAPPGSTGSSLVVTEYEQPYGGFLDILRGGTERLFTNIKDTSSKVIQSVANYAKGDLDISYITSRIAVMSFPAEGVESAIKNNIEDVRLFLDSKHPGRYAVYNLSPRTYRPSKFHNRVSDCGWVARRAPSLRSLYSLCRNMHAWLRRDPGNVCVVHCMDGRAVSAVAVCSFLCFCRLFSTAEAAVYMFSMKRCPPGIWPSHKRYIEYMCDMVAEEPVTPHSKPILVKAVVMTPVPLFSKQRSGCRPFCEVYVGDERVTTTSQEYDKMRDFKIEDGKAVIPLGITVQGDVLIIIYHARSTLGGRLQAKMASMKMFQIQFHTGFVPRNAATVKFAKYDLDACDIQEKYPDLFQVSLEVEVEPRDRPGHEAPPWEGAGMRGLNPKILFSSREEQQDVLSKFGKPELPRQPGSTAQYDAEAGPADAEPPESDSPHSSVDADHFLHTLDWREDRDSSVGPEGHLAEEGPSAVAEDGCVSEPSDEEAPTPSAESGDTGDEDVPAPAAPQDDSVDLLGLHAEAGPVPAPQAPGGPPSNADLLGCLLGAPGPAPEGAVGGLLDGDAALLFSSPAPAPCTQTTAPAADPFGPLLLPPDVDTPPSSQPDLFGEFLNSDAPAAQPAPFPSTHSAPPPACSTDFLHLGDLPAEPSKMTASASHPDLLGAWDAWAEAPAPALAADGTFFSPGDQPAPSGLPAGQTKSQSQDPFADLGDLSAGLQGSPAAAAPGGFGPKAAPPPKASSSWQTSRPPAQGTPWPPQAKPPPKASVPPRPNYTSSFSVIGSREERGVRAPSFGPKPRVSENDFEDLLSNQGFSSRADRKGPRTIAEMRRQDQARDLDPLKLKLLEWTEGKERNIRALLSTLHTALWDGESRWTPVGMADLVTPAQVKKHYRRAVLVVHPDKARGQPYEQYARMIFMELNDAWAEFESQGSRPLF

Target ORF information:

RefSeq Version	XM_010806358.2
Organism	Bos taurus(cattle)
Definition	Bos taurus cyclin G associated kinase (GAK), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010806358.2

ORF Insert Sequence:

ATGAAAAAAC TTTCAGGCCA CCCAAACATT GTCCAGTTTT GTTCTGCAGC ATCGATAGGA 
AAAGAGGAGT CGGACACTGG GCAGGCGGAG TTCCTGCTGC TCATGGAGCT CTGCAGAGGG 
CAGCTGGTGG AATTTTTAAA AAAAGTTGAA TCGAAAGGTC CGCTCTCGTG CGACACCGTC 
CTGAAGATCT TCTACCAGAC GTGCCGGGCG GTGCAGCACA TGCACAGACA GAAGCCGCCC 
ATCATCCACA GGGACCTCAA GGTTGAAAAC TTACTGCTTA GTAACCAGGG GACCATCAAG 
CTGTGCGACT TCGGCAGTGC CACGACCATC TCACACTACC CAGACTACAG CTGGAGCGCC 
CAGCGCCGGG CCCTGGTGGA GGAGGAGATC ACCAGGAACA CGACCCCCAT GTACAGGACG 
CCGGAAATTG TGGACTTGTA CTCAAACTTT CCGATTGGCG AGAAGCAGGA CATCTGGGCC 
CTGGGCTGCA TCCTGTACCT GCTCTGCTTC GGGCAGCACC CCTTTGAGGA CGGAGCGAAG 
CTTCGCATCG TCAACGGGAA GTACGCAATC CCTGCCGACG ACACGCGGTA CTCCGTGTTC 
CACAGCCTCA TCCGTTCCAT GCTCAAGGTC AACCCGGAGG AGCGGCTGTC CGTCACCGAG 
CTGGTCAACC AGCTCCAGGA GATCGCGGCC GCCCGCAACG TGAACCCCAA GTCACCCATC 
ACTGAGCTCC TGGAGCAGAA TGGAGGCTAT GGGCACGCGG CCCCTCCCGG CTCCACGGGC 
AGCAGTCTGG TGGTCACTGA GTACGAGCAG CCCTATGGCG GGTTCCTGGA CATTCTGCGT 
GGGGGCACCG AGCGCCTCTT CACCAACATC AAGGACACCT CCTCCAAAGT CATCCAGTCC 
GTTGCCAACT ATGCGAAGGG AGATCTGGAT ATTTCTTACA TCACGTCTAG AATCGCAGTC 
ATGTCGTTCC CGGCGGAGGG CGTGGAGTCG GCCATCAAGA ACAACATTGA GGATGTACGG 
CTGTTCCTGG ACTCGAAGCA CCCGGGCCGC TACGCCGTCT ACAACCTGTC CCCGCGGACC 
TACCGGCCCT CGAAGTTCCA CAACCGGGTC TCCGACTGCG GCTGGGTGGC CCGGCGGGCC 
CCGAGCCTCC GCAGCCTGTA CAGCCTCTGC AGGAACATGC ACGCCTGGCT GCGCCGCGAC 
CCTGGCAACG TCTGCGTGGT GCACTGCATG GACGGGAGGG CGGTGTCGGC CGTGGCCGTG 
TGCTCCTTCT TGTGCTTCTG CCGGCTCTTC AGCACCGCGG AGGCCGCTGT GTACATGTTC 
AGCATGAAGC GGTGCCCACC GGGCATCTGG CCATCCCACA AGAGGTACAT TGAGTACATG 
TGTGACATGG TGGCCGAGGA GCCCGTCACG CCCCACAGCA AGCCCATCCT GGTGAAGGCC 
GTGGTCATGA CGCCTGTGCC GCTCTTCAGC AAGCAGAGGA GCGGCTGCAG GCCCTTCTGC 
GAGGTCTACG TGGGGGATGA GCGCGTGACC ACCACGTCCC AGGAATACGA CAAGATGAGA 
GACTTTAAAA TTGAGGATGG CAAAGCGGTC ATTCCCCTGG GCATAACAGT CCAGGGGGAC 
GTGCTCATCA TCATCTATCA TGCGAGGTCC ACCCTGGGAG GAAGGCTGCA GGCCAAGATG 
GCGTCCATGA AGATGTTTCA GATCCAGTTT CACACAGGGT TCGTGCCTCG GAACGCAGCT 
ACTGTGAAGT TTGCCAAGTA TGATCTGGAT GCTTGCGACA TCCAGGAGAA GTACCCCGAC 
TTGTTCCAAG TGAGCCTGGA GGTGGAGGTG GAGCCCAGAG ACAGGCCCGG CCACGAGGCC 
CCGCCCTGGG AGGGTGCAGG CATGCGAGGG CTGAACCCCA AGATCCTCTT CTCCAGCCGG 
GAGGAGCAGC AAGATGTCCT GTCCAAGTTT GGGAAGCCAG AGCTCCCCAG GCAGCCTGGG 
TCCACCGCAC AGTACGACGC CGAGGCCGGG CCCGCGGATG CCGAGCCCCC CGAGTCAGAC 
TCGCCGCACA GCAGCGTGGA TGCTGACCAC TTCCTGCACA CGCTGGACTG GCGGGAGGAT 
CGGGACTCCA GTGTGGGCCC GGAGGGTCAC CTGGCCGAGG AGGGTCCCTC TGCTGTGGCC 
GAGGACGGCT GTGTGAGTGA GCCATCGGAC GAGGAAGCCC CGACACCCTC TGCGGAGAGC 
GGGGACACAG GCGATGAGGA CGTGCCCGCC CCCGCCGCCC CGCAGGACGA CAGCGTGGAC 
CTTCTGGGGC TGCACGCGGA GGCGGGGCCT GTGCCCGCCC CCCAGGCCCC CGGCGGCCCC 
CCCAGCAATG CAGACCTGCT CGGCTGCCTG CTCGGGGCCC CGGGCCCCGC TCCGGAGGGT 
GCTGTGGGGG GCCTGCTCGA CGGGGATGCG GCCCTGCTCT TCAGCAGCCC AGCCCCCGCG 
CCTTGCACCC AGACAACTGC CCCGGCCGCT GACCCGTTCG GCCCGCTCCT GCTGCCGCCC 
GACGTGGACA CCCCTCCTAG CTCGCAGCCT GACCTCTTCG GGGAATTCCT CAACTCAGAC 
GCCCCGGCCG CCCAGCCCGC GCCCTTCCCC TCCACCCACA GCGCTCCGCC GCCAGCCTGC 
AGCACCGACT TCCTGCATCT TGGGGACCTG CCCGCCGAGC CCAGCAAGAT GACAGCCTCT 
GCCAGCCACC CCGACCTGCT GGGAGCCTGG GATGCCTGGG CTGAGGCGCC GGCCCCCGCG 
CTGGCTGCAG ACGGCACCTT CTTCTCTCCT GGAGACCAGC CAGCCCCTTC TGGCCTTCCG 
GCTGGCCAGA CCAAGTCTCA GAGCCAGGAC CCGTTTGCAG ACCTTGGCGA CCTCAGTGCC 
GGCCTCCAAG GCTCGCCTGC CGCAGCTGCT CCTGGGGGCT TTGGTCCCAA AGCAGCGCCC 
CCTCCCAAGG CCAGCAGCTC CTGGCAGACG AGTCGGCCCC CAGCCCAGGG CACCCCGTGG 
CCCCCCCAGG CCAAGCCGCC CCCCAAAGCC AGTGTACCGC CAAGGCCTAA CTACACCTCC 
AGCTTCAGTG TGATCGGTAG CCGGGAGGAG CGGGGGGTCC GCGCCCCCAG CTTTGGTCCA 
AAGCCGAGGG TGTCAGAAAA CGACTTTGAA GACCTGCTCT CCAACCAGGG CTTCTCCTCG 
CGGGCTGACA GGAAGGGGCC GCGGACCATC GCTGAGATGA GGAGGCAGGA CCAGGCCCGG 
GACTTGGATC CGCTGAAGCT GAAGCTTCTG GAGTGGACGG AGGGCAAGGA GAGGAACATC 
CGCGCCCTGC TGTCCACGCT GCACACGGCG CTGTGGGACG GCGAGAGCCG CTGGACGCCG 
GTGGGCATGG CCGACCTGGT GACGCCCGCA CAAGTGAAGA AGCACTACCG CCGTGCCGTG 
CTGGTGGTCC ACCCCGACAA GGCCCGGGGG CAGCCCTACG AGCAGTATGC CCGGATGATC 
TTCATGGAGC TCAACGATGC GTGGGCTGAG TTTGAGAGCC AGGGCTCCCG GCCACTCTTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAK ( XM_015471751.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015327237.1 Bos taurus cyclin G associated kinase (GAK), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa20663
Clone ID Related Accession (Same CDS sequence)	XM_015471751.1 , XM_005208283.1
Accession Version	XM_015471751.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3441bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	cyclin-G-associated kinase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103986.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTCT GCAGAGGGCA GCTGGTGGAA TTTTTAAAAA AAGTTGAATC GAAAGGTCCG 
CTCTCGTGCG ACACCGTCCT GAAGATCTTC TACCAGACGT GCCGGGCGGT GCAGCACATG 
CACAGACAGA AGCCGCCCAT CATCCACAGG GACCTCAAGG TTGAAAACTT ACTGCTTAGT 
AACCAGGGGA CCATCAAGCT GTGCGACTTC GGCAGTGCCA CGACCATCTC ACACTACCCA 
GACTACAGCT GGAGCGCCCA GCGCCGGGCC CTGGTGGAGG AGGAGATCAC CAGGAACACG 
ACCCCCATGT ACAGGACGCC GGAAATTGTG GACTTGTACT CAAACTTTCC GATTGGCGAG 
AAGCAGGACA TCTGGGCCCT GGGCTGCATC CTGTACCTGC TCTGCTTCGG GCAGCACCCC 
TTTGAGGACG GAGCGAAGCT TCGCATCGTC AACGGGAAGT ACGCAATCCC TGCCGACGAC 
ACGCGGTACT CCGTGTTCCA CAGCCTCATC CGTTCCATGC TCAAGGTCAA CCCGGAGGAG 
CGGCTGTCCG TCACCGAGCT GGTCAACCAG CTCCAGGAGA TCGCGGCCGC CCGCAACGTG 
AACCCCAAGT CACCCATCAC TGAGCTCCTG GAGCAGAATG GAGGCTATGG GCACGCGGCC 
CCTCCCGGCT CCACGGGCAG CAGTCTGGTG GTCACTGAGT ACGAGCAGCC CTATGGCGGG 
TTCCTGGACA TTCTGCGTGG GGGCACCGAG CGCCTCTTCA CCAACATCAA GGACACCTCC 
TCCAAAGTCA TCCAGTCCGT TGCCAACTAT GCGAAGGGAG ATCTGGATAT TTCTTACATC 
ACGTCTAGAA TCGCAGTCAT GTCGTTCCCG GCGGAGGGCG TGGAGTCGGC CATCAAGAAC 
AACATTGAGG ATGTACGGCT GTTCCTGGAC TCGAAGCACC CGGGCCGCTA CGCCGTCTAC 
AACCTGTCCC CGCGGACCTA CCGGCCCTCG AAGTTCCACA ACCGGGTCTC CGACTGCGGC 
TGGGTGGCCC GGCGGGCCCC GAGCCTCCGC AGCCTGTACA GCCTCTGCAG GAACATGCAC 
GCCTGGCTGC GCCGCGACCC TGGCAACGTC TGCGTGGTGC ACTGCATGGA CGGGAGGGCG 
GTGTCGGCCG TGGCCGTGTG CTCCTTCTTG TGCTTCTGCC GGCTCTTCAG CACCGCGGAG 
GCCGCTGTGT ACATGTTCAG CATGAAGCGG TGCCCACCGG GCATCTGGCC ATCCCACAAG 
AGGTACATTG AGTACATGTG TGACATGGTG GCCGAGGAGC CCGTCACGCC CCACAGCAAG 
CCCATCCTGG TGAAGGCCGT GGTCATGACG CCTGTGCCGC TCTTCAGCAA GCAGAGGAGC 
GGCTGCAGGC CCTTCTGCGA GGTCTACGTG GGGGATGAGC GCGTGACCAC CACGTCCCAG 
GAATACGACA AGATGAGAGA CTTTAAAATT GAGGATGGCA AAGCGGTCAT TCCCCTGGGC 
ATAACAGTCC AGGGGGACGT GCTCATCATC ATCTATCATG CGAGGTCCAC CCTGGGAGGA 
AGGCTGCAGG CCAAGATGGC GTCCATGAAG ATGTTTCAGA TCCAGTTTCA CACAGGGTTC 
GTGCCTCGGA ACGCAGCTAC TGTGAAGTTT GCCAAGTATG ATCTGGATGC TTGCGACATC 
CAGGAGAAGT ACCCCGACTT GTTCCAAGTG AGCCTGGAGG TGGAGGTGGA GCCCAGAGAC 
AGGCCCGGCC ACGAGGCCCC GCCCTGGGAG GGTGCAGGCA TGCGAGGGCT GAACCCCAAG 
ATCCTCTTCT CCAGCCGGGA GGAGCAGCAA GATGTCCTGT CCAAGTTTGG GAAGCCAGAG 
CTCCCCAGGC AGCCTGGGTC CACCGCACAG TACGACGCCG AGGCCGGGCC CGCGGATGCC 
GAGCCCCCCG AGTCAGACTC GCCGCACAGC AGCGTGGATG CTGACCACTT CCTGCACACG 
CTGGACTGGC GGGAGGATCG GGACTCCAGT GTGGGCCCGG AGGGTCACCT GGCCGAGGAG 
GGTCCCTCTG CTGTGGCCGA GGACGGCTGT GTGAGTGAGC CATCGGACGA GGAAGCCCCG 
ACACCCTCTG CGGAGAGCGG GGACACAGGC GATGAGGACG TGCCCGCCCC CGCCGCCCCG 
CAGGACGACA GCGTGGACCT TCTGGGGCTG CACGCGGAGG CGGGGCCTGT GCCCGCCCCC 
CAGGCCCCCG GCGGCCCCCC CAGCAATGCA GACCTGCTCG GCTGCCTGCT CGGGGCCCCG 
GGCCCCGCTC CGGAGGGTGC TGTGGGGGGC CTGCTCGACG GGGATGCGGC CCTGCTCTTC 
AGCAGCCCAG CCCCCGCGCC TTGCACCCAG ACAACTGCCC CGGCCGCTGA CCCGTTCGGC 
CCGCTCCTGC TGCCGCCCGA CGTGGACACC CCTCCTAGCT CGCAGCCTGA CCTCTTCGGG 
GAATTCCTCA ACTCAGACGC CCCGGCCGCC CAGCCCGCGC CCTTCCCCTC CACCCACAGC 
GCTCCGCCGC CAGCCTGCAG CACCGACTTC CTGCATCTTG GGGACCTGCC CGCCGAGCCC 
AGCAAGATGA CAGCCTCTGC CAGCCACCCC GACCTGCTGG GAGCCTGGGA TGCCTGGGCT 
GAGGCGCCGG CCCCCGCGCT GGCTGCAGAC GGCACCTTCT TCTCTCCTGG AGACCAGCCA 
GCCCCTTCTG GCCTTCCGGC TGGCCAGACC AAGTCTCAGA GCCAGGACCC GTTTGCAGAC 
CTTGGCGACC TCAGTGCCGG CCTCCAAGGC TCGCCTGCCG CAGCTGCTCC TGGGGGCTTT 
GGTCCCAAAG CAGCGCCCCC TCCCAAGGCC AGCAGCTCCT GGCAGACGAG TCGGCCCCCA 
GCCCAGGGCA CCCCGTGGCC CCCCCAGGCC AAGCCGCCCC CCAAAGCCAG TGTACCGCCA 
AGGCCTAACT ACACCTCCAG CTTCAGTGTG ATCGGTAGCC GGGAGGAGCG GGGGGTCCGC 
GCCCCCAGCT TTGGTCCAAA GCCGAGGGTG TCAGAAAACG ACTTTGAAGA CCTGCTCTCC 
AACCAGGGCT TCTCCTCGCG GGCTGACAGG AAGGGGCCGC GGACCATCGC TGAGATGAGG 
AGGCAGGACC AGGCCCGGGA CTTGGATCCG CTGAAGCTGA AGCTTCTGGA GTGGACGGAG 
GGCAAGGAGA GGAACATCCG CGCCCTGCTG TCCACGCTGC ACACGGCGCT GTGGGACGGC 
GAGAGCCGCT GGACGCCGGT GGGCATGGCC GACCTGGTGA CGCCCGCACA AGTGAAGAAG 
CACTACCGCC GTGCCGTGCT GGTGGTCCAC CCCGACAAGG CCCGGGGGCA GCCCTACGAG 
CAGTATGCCC GGATGATCTT CATGGAGCTC AACGATGCGT GGGCTGAGTT TGAGAGCCAG 
GGCTCCCGGC CACTCTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015327237.1
CDS	340..3780
Translation	MELCRGQLVEFLKKVESKGPLSCDTVLKIFYQTCRAVQHMHRQKPPIIHRDLKVENLLLSNQGTIKLCDFGSATTISHYPDYSWSAQRRALVEEEITRNTTPMYRTPEIVDLYSNFPIGEKQDIWALGCILYLLCFGQHPFEDGAKLRIVNGKYAIPADDTRYSVFHSLIRSMLKVNPEERLSVTELVNQLQEIAAARNVNPKSPITELLEQNGGYGHAAPPGSTGSSLVVTEYEQPYGGFLDILRGGTERLFTNIKDTSSKVIQSVANYAKGDLDISYITSRIAVMSFPAEGVESAIKNNIEDVRLFLDSKHPGRYAVYNLSPRTYRPSKFHNRVSDCGWVARRAPSLRSLYSLCRNMHAWLRRDPGNVCVVHCMDGRAVSAVAVCSFLCFCRLFSTAEAAVYMFSMKRCPPGIWPSHKRYIEYMCDMVAEEPVTPHSKPILVKAVVMTPVPLFSKQRSGCRPFCEVYVGDERVTTTSQEYDKMRDFKIEDGKAVIPLGITVQGDVLIIIYHARSTLGGRLQAKMASMKMFQIQFHTGFVPRNAATVKFAKYDLDACDIQEKYPDLFQVSLEVEVEPRDRPGHEAPPWEGAGMRGLNPKILFSSREEQQDVLSKFGKPELPRQPGSTAQYDAEAGPADAEPPESDSPHSSVDADHFLHTLDWREDRDSSVGPEGHLAEEGPSAVAEDGCVSEPSDEEAPTPSAESGDTGDEDVPAPAAPQDDSVDLLGLHAEAGPVPAPQAPGGPPSNADLLGCLLGAPGPAPEGAVGGLLDGDAALLFSSPAPAPCTQTTAPAADPFGPLLLPPDVDTPPSSQPDLFGEFLNSDAPAAQPAPFPSTHSAPPPACSTDFLHLGDLPAEPSKMTASASHPDLLGAWDAWAEAPAPALAADGTFFSPGDQPAPSGLPAGQTKSQSQDPFADLGDLSAGLQGSPAAAAPGGFGPKAAPPPKASSSWQTSRPPAQGTPWPPQAKPPPKASVPPRPNYTSSFSVIGSREERGVRAPSFGPKPRVSENDFEDLLSNQGFSSRADRKGPRTIAEMRRQDQARDLDPLKLKLLEWTEGKERNIRALLSTLHTALWDGESRWTPVGMADLVTPAQVKKHYRRAVLVVHPDKARGQPYEQYARMIFMELNDAWAEFESQGSRPLF

Target ORF information:

RefSeq Version	XM_015471751.1
Organism	Bos taurus(cattle)
Definition	Bos taurus cyclin G associated kinase (GAK), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015471751.1

ORF Insert Sequence:

ATGGAGCTCT GCAGAGGGCA GCTGGTGGAA TTTTTAAAAA AAGTTGAATC GAAAGGTCCG 
CTCTCGTGCG ACACCGTCCT GAAGATCTTC TACCAGACGT GCCGGGCGGT GCAGCACATG 
CACAGACAGA AGCCGCCCAT CATCCACAGG GACCTCAAGG TTGAAAACTT ACTGCTTAGT 
AACCAGGGGA CCATCAAGCT GTGCGACTTC GGCAGTGCCA CGACCATCTC ACACTACCCA 
GACTACAGCT GGAGCGCCCA GCGCCGGGCC CTGGTGGAGG AGGAGATCAC CAGGAACACG 
ACCCCCATGT ACAGGACGCC GGAAATTGTG GACTTGTACT CAAACTTTCC GATTGGCGAG 
AAGCAGGACA TCTGGGCCCT GGGCTGCATC CTGTACCTGC TCTGCTTCGG GCAGCACCCC 
TTTGAGGACG GAGCGAAGCT TCGCATCGTC AACGGGAAGT ACGCAATCCC TGCCGACGAC 
ACGCGGTACT CCGTGTTCCA CAGCCTCATC CGTTCCATGC TCAAGGTCAA CCCGGAGGAG 
CGGCTGTCCG TCACCGAGCT GGTCAACCAG CTCCAGGAGA TCGCGGCCGC CCGCAACGTG 
AACCCCAAGT CACCCATCAC TGAGCTCCTG GAGCAGAATG GAGGCTATGG GCACGCGGCC 
CCTCCCGGCT CCACGGGCAG CAGTCTGGTG GTCACTGAGT ACGAGCAGCC CTATGGCGGG 
TTCCTGGACA TTCTGCGTGG GGGCACCGAG CGCCTCTTCA CCAACATCAA GGACACCTCC 
TCCAAAGTCA TCCAGTCCGT TGCCAACTAT GCGAAGGGAG ATCTGGATAT TTCTTACATC 
ACGTCTAGAA TCGCAGTCAT GTCGTTCCCG GCGGAGGGCG TGGAGTCGGC CATCAAGAAC 
AACATTGAGG ATGTACGGCT GTTCCTGGAC TCGAAGCACC CGGGCCGCTA CGCCGTCTAC 
AACCTGTCCC CGCGGACCTA CCGGCCCTCG AAGTTCCACA ACCGGGTCTC CGACTGCGGC 
TGGGTGGCCC GGCGGGCCCC GAGCCTCCGC AGCCTGTACA GCCTCTGCAG GAACATGCAC 
GCCTGGCTGC GCCGCGACCC TGGCAACGTC TGCGTGGTGC ACTGCATGGA CGGGAGGGCG 
GTGTCGGCCG TGGCCGTGTG CTCCTTCTTG TGCTTCTGCC GGCTCTTCAG CACCGCGGAG 
GCCGCTGTGT ACATGTTCAG CATGAAGCGG TGCCCACCGG GCATCTGGCC ATCCCACAAG 
AGGTACATTG AGTACATGTG TGACATGGTG GCCGAGGAGC CCGTCACGCC CCACAGCAAG 
CCCATCCTGG TGAAGGCCGT GGTCATGACG CCTGTGCCGC TCTTCAGCAA GCAGAGGAGC 
GGCTGCAGGC CCTTCTGCGA GGTCTACGTG GGGGATGAGC GCGTGACCAC CACGTCCCAG 
GAATACGACA AGATGAGAGA CTTTAAAATT GAGGATGGCA AAGCGGTCAT TCCCCTGGGC 
ATAACAGTCC AGGGGGACGT GCTCATCATC ATCTATCATG CGAGGTCCAC CCTGGGAGGA 
AGGCTGCAGG CCAAGATGGC GTCCATGAAG ATGTTTCAGA TCCAGTTTCA CACAGGGTTC 
GTGCCTCGGA ACGCAGCTAC TGTGAAGTTT GCCAAGTATG ATCTGGATGC TTGCGACATC 
CAGGAGAAGT ACCCCGACTT GTTCCAAGTG AGCCTGGAGG TGGAGGTGGA GCCCAGAGAC 
AGGCCCGGCC ACGAGGCCCC GCCCTGGGAG GGTGCAGGCA TGCGAGGGCT GAACCCCAAG 
ATCCTCTTCT CCAGCCGGGA GGAGCAGCAA GATGTCCTGT CCAAGTTTGG GAAGCCAGAG 
CTCCCCAGGC AGCCTGGGTC CACCGCACAG TACGACGCCG AGGCCGGGCC CGCGGATGCC 
GAGCCCCCCG AGTCAGACTC GCCGCACAGC AGCGTGGATG CTGACCACTT CCTGCACACG 
CTGGACTGGC GGGAGGATCG GGACTCCAGT GTGGGCCCGG AGGGTCACCT GGCCGAGGAG 
GGTCCCTCTG CTGTGGCCGA GGACGGCTGT GTGAGTGAGC CATCGGACGA GGAAGCCCCG 
ACACCCTCTG CGGAGAGCGG GGACACAGGC GATGAGGACG TGCCCGCCCC CGCCGCCCCG 
CAGGACGACA GCGTGGACCT TCTGGGGCTG CACGCGGAGG CGGGGCCTGT GCCCGCCCCC 
CAGGCCCCCG GCGGCCCCCC CAGCAATGCA GACCTGCTCG GCTGCCTGCT CGGGGCCCCG 
GGCCCCGCTC CGGAGGGTGC TGTGGGGGGC CTGCTCGACG GGGATGCGGC CCTGCTCTTC 
AGCAGCCCAG CCCCCGCGCC TTGCACCCAG ACAACTGCCC CGGCCGCTGA CCCGTTCGGC 
CCGCTCCTGC TGCCGCCCGA CGTGGACACC CCTCCTAGCT CGCAGCCTGA CCTCTTCGGG 
GAATTCCTCA ACTCAGACGC CCCGGCCGCC CAGCCCGCGC CCTTCCCCTC CACCCACAGC 
GCTCCGCCGC CAGCCTGCAG CACCGACTTC CTGCATCTTG GGGACCTGCC CGCCGAGCCC 
AGCAAGATGA CAGCCTCTGC CAGCCACCCC GACCTGCTGG GAGCCTGGGA TGCCTGGGCT 
GAGGCGCCGG CCCCCGCGCT GGCTGCAGAC GGCACCTTCT TCTCTCCTGG AGACCAGCCA 
GCCCCTTCTG GCCTTCCGGC TGGCCAGACC AAGTCTCAGA GCCAGGACCC GTTTGCAGAC 
CTTGGCGACC TCAGTGCCGG CCTCCAAGGC TCGCCTGCCG CAGCTGCTCC TGGGGGCTTT 
GGTCCCAAAG CAGCGCCCCC TCCCAAGGCC AGCAGCTCCT GGCAGACGAG TCGGCCCCCA 
GCCCAGGGCA CCCCGTGGCC CCCCCAGGCC AAGCCGCCCC CCAAAGCCAG TGTACCGCCA 
AGGCCTAACT ACACCTCCAG CTTCAGTGTG ATCGGTAGCC GGGAGGAGCG GGGGGTCCGC 
GCCCCCAGCT TTGGTCCAAA GCCGAGGGTG TCAGAAAACG ACTTTGAAGA CCTGCTCTCC 
AACCAGGGCT TCTCCTCGCG GGCTGACAGG AAGGGGCCGC GGACCATCGC TGAGATGAGG 
AGGCAGGACC AGGCCCGGGA CTTGGATCCG CTGAAGCTGA AGCTTCTGGA GTGGACGGAG 
GGCAAGGAGA GGAACATCCG CGCCCTGCTG TCCACGCTGC ACACGGCGCT GTGGGACGGC 
GAGAGCCGCT GGACGCCGGT GGGCATGGCC GACCTGGTGA CGCCCGCACA AGTGAAGAAG 
CACTACCGCC GTGCCGTGCT GGTGGTCCAC CCCGACAAGG CCCGGGGGCA GCCCTACGAG 
CAGTATGCCC GGATGATCTT CATGGAGCTC AACGATGCGT GGGCTGAGTT TGAGAGCCAG 
GGCTCCCGGC CACTCTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAK ( XM_005208283.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005208340.1 Bos taurus cyclin G associated kinase (GAK), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa20663
Clone ID Related Accession (Same CDS sequence)	XM_015471751.1 , XM_005208283.1
Accession Version	XM_005208283.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3441bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	cyclin-G-associated kinase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103986.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTCT GCAGAGGGCA GCTGGTGGAA TTTTTAAAAA AAGTTGAATC GAAAGGTCCG 
CTCTCGTGCG ACACCGTCCT GAAGATCTTC TACCAGACGT GCCGGGCGGT GCAGCACATG 
CACAGACAGA AGCCGCCCAT CATCCACAGG GACCTCAAGG TTGAAAACTT ACTGCTTAGT 
AACCAGGGGA CCATCAAGCT GTGCGACTTC GGCAGTGCCA CGACCATCTC ACACTACCCA 
GACTACAGCT GGAGCGCCCA GCGCCGGGCC CTGGTGGAGG AGGAGATCAC CAGGAACACG 
ACCCCCATGT ACAGGACGCC GGAAATTGTG GACTTGTACT CAAACTTTCC GATTGGCGAG 
AAGCAGGACA TCTGGGCCCT GGGCTGCATC CTGTACCTGC TCTGCTTCGG GCAGCACCCC 
TTTGAGGACG GAGCGAAGCT TCGCATCGTC AACGGGAAGT ACGCAATCCC TGCCGACGAC 
ACGCGGTACT CCGTGTTCCA CAGCCTCATC CGTTCCATGC TCAAGGTCAA CCCGGAGGAG 
CGGCTGTCCG TCACCGAGCT GGTCAACCAG CTCCAGGAGA TCGCGGCCGC CCGCAACGTG 
AACCCCAAGT CACCCATCAC TGAGCTCCTG GAGCAGAATG GAGGCTATGG GCACGCGGCC 
CCTCCCGGCT CCACGGGCAG CAGTCTGGTG GTCACTGAGT ACGAGCAGCC CTATGGCGGG 
TTCCTGGACA TTCTGCGTGG GGGCACCGAG CGCCTCTTCA CCAACATCAA GGACACCTCC 
TCCAAAGTCA TCCAGTCCGT TGCCAACTAT GCGAAGGGAG ATCTGGATAT TTCTTACATC 
ACGTCTAGAA TCGCAGTCAT GTCGTTCCCG GCGGAGGGCG TGGAGTCGGC CATCAAGAAC 
AACATTGAGG ATGTACGGCT GTTCCTGGAC TCGAAGCACC CGGGCCGCTA CGCCGTCTAC 
AACCTGTCCC CGCGGACCTA CCGGCCCTCG AAGTTCCACA ACCGGGTCTC CGACTGCGGC 
TGGGTGGCCC GGCGGGCCCC GAGCCTCCGC AGCCTGTACA GCCTCTGCAG GAACATGCAC 
GCCTGGCTGC GCCGCGACCC TGGCAACGTC TGCGTGGTGC ACTGCATGGA CGGGAGGGCG 
GTGTCGGCCG TGGCCGTGTG CTCCTTCTTG TGCTTCTGCC GGCTCTTCAG CACCGCGGAG 
GCCGCTGTGT ACATGTTCAG CATGAAGCGG TGCCCACCGG GCATCTGGCC ATCCCACAAG 
AGGTACATTG AGTACATGTG TGACATGGTG GCCGAGGAGC CCGTCACGCC CCACAGCAAG 
CCCATCCTGG TGAAGGCCGT GGTCATGACG CCTGTGCCGC TCTTCAGCAA GCAGAGGAGC 
GGCTGCAGGC CCTTCTGCGA GGTCTACGTG GGGGATGAGC GCGTGACCAC CACGTCCCAG 
GAATACGACA AGATGAGAGA CTTTAAAATT GAGGATGGCA AAGCGGTCAT TCCCCTGGGC 
ATAACAGTCC AGGGGGACGT GCTCATCATC ATCTATCATG CGAGGTCCAC CCTGGGAGGA 
AGGCTGCAGG CCAAGATGGC GTCCATGAAG ATGTTTCAGA TCCAGTTTCA CACAGGGTTC 
GTGCCTCGGA ACGCAGCTAC TGTGAAGTTT GCCAAGTATG ATCTGGATGC TTGCGACATC 
CAGGAGAAGT ACCCCGACTT GTTCCAAGTG AGCCTGGAGG TGGAGGTGGA GCCCAGAGAC 
AGGCCCGGCC ACGAGGCCCC GCCCTGGGAG GGTGCAGGCA TGCGAGGGCT GAACCCCAAG 
ATCCTCTTCT CCAGCCGGGA GGAGCAGCAA GATGTCCTGT CCAAGTTTGG GAAGCCAGAG 
CTCCCCAGGC AGCCTGGGTC CACCGCACAG TACGACGCCG AGGCCGGGCC CGCGGATGCC 
GAGCCCCCCG AGTCAGACTC GCCGCACAGC AGCGTGGATG CTGACCACTT CCTGCACACG 
CTGGACTGGC GGGAGGATCG GGACTCCAGT GTGGGCCCGG AGGGTCACCT GGCCGAGGAG 
GGTCCCTCTG CTGTGGCCGA GGACGGCTGT GTGAGTGAGC CATCGGACGA GGAAGCCCCG 
ACACCCTCTG CGGAGAGCGG GGACACAGGC GATGAGGACG TGCCCGCCCC CGCCGCCCCG 
CAGGACGACA GCGTGGACCT TCTGGGGCTG CACGCGGAGG CGGGGCCTGT GCCCGCCCCC 
CAGGCCCCCG GCGGCCCCCC CAGCAATGCA GACCTGCTCG GCTGCCTGCT CGGGGCCCCG 
GGCCCCGCTC CGGAGGGTGC TGTGGGGGGC CTGCTCGACG GGGATGCGGC CCTGCTCTTC 
AGCAGCCCAG CCCCCGCGCC TTGCACCCAG ACAACTGCCC CGGCCGCTGA CCCGTTCGGC 
CCGCTCCTGC TGCCGCCCGA CGTGGACACC CCTCCTAGCT CGCAGCCTGA CCTCTTCGGG 
GAATTCCTCA ACTCAGACGC CCCGGCCGCC CAGCCCGCGC CCTTCCCCTC CACCCACAGC 
GCTCCGCCGC CAGCCTGCAG CACCGACTTC CTGCATCTTG GGGACCTGCC CGCCGAGCCC 
AGCAAGATGA CAGCCTCTGC CAGCCACCCC GACCTGCTGG GAGCCTGGGA TGCCTGGGCT 
GAGGCGCCGG CCCCCGCGCT GGCTGCAGAC GGCACCTTCT TCTCTCCTGG AGACCAGCCA 
GCCCCTTCTG GCCTTCCGGC TGGCCAGACC AAGTCTCAGA GCCAGGACCC GTTTGCAGAC 
CTTGGCGACC TCAGTGCCGG CCTCCAAGGC TCGCCTGCCG CAGCTGCTCC TGGGGGCTTT 
GGTCCCAAAG CAGCGCCCCC TCCCAAGGCC AGCAGCTCCT GGCAGACGAG TCGGCCCCCA 
GCCCAGGGCA CCCCGTGGCC CCCCCAGGCC AAGCCGCCCC CCAAAGCCAG TGTACCGCCA 
AGGCCTAACT ACACCTCCAG CTTCAGTGTG ATCGGTAGCC GGGAGGAGCG GGGGGTCCGC 
GCCCCCAGCT TTGGTCCAAA GCCGAGGGTG TCAGAAAACG ACTTTGAAGA CCTGCTCTCC 
AACCAGGGCT TCTCCTCGCG GGCTGACAGG AAGGGGCCGC GGACCATCGC TGAGATGAGG 
AGGCAGGACC AGGCCCGGGA CTTGGATCCG CTGAAGCTGA AGCTTCTGGA GTGGACGGAG 
GGCAAGGAGA GGAACATCCG CGCCCTGCTG TCCACGCTGC ACACGGCGCT GTGGGACGGC 
GAGAGCCGCT GGACGCCGGT GGGCATGGCC GACCTGGTGA CGCCCGCACA AGTGAAGAAG 
CACTACCGCC GTGCCGTGCT GGTGGTCCAC CCCGACAAGG CCCGGGGGCA GCCCTACGAG 
CAGTATGCCC GGATGATCTT CATGGAGCTC AACGATGCGT GGGCTGAGTT TGAGAGCCAG 
GGCTCCCGGC CACTCTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005208340.1
CDS	663..4103
Translation	MELCRGQLVEFLKKVESKGPLSCDTVLKIFYQTCRAVQHMHRQKPPIIHRDLKVENLLLSNQGTIKLCDFGSATTISHYPDYSWSAQRRALVEEEITRNTTPMYRTPEIVDLYSNFPIGEKQDIWALGCILYLLCFGQHPFEDGAKLRIVNGKYAIPADDTRYSVFHSLIRSMLKVNPEERLSVTELVNQLQEIAAARNVNPKSPITELLEQNGGYGHAAPPGSTGSSLVVTEYEQPYGGFLDILRGGTERLFTNIKDTSSKVIQSVANYAKGDLDISYITSRIAVMSFPAEGVESAIKNNIEDVRLFLDSKHPGRYAVYNLSPRTYRPSKFHNRVSDCGWVARRAPSLRSLYSLCRNMHAWLRRDPGNVCVVHCMDGRAVSAVAVCSFLCFCRLFSTAEAAVYMFSMKRCPPGIWPSHKRYIEYMCDMVAEEPVTPHSKPILVKAVVMTPVPLFSKQRSGCRPFCEVYVGDERVTTTSQEYDKMRDFKIEDGKAVIPLGITVQGDVLIIIYHARSTLGGRLQAKMASMKMFQIQFHTGFVPRNAATVKFAKYDLDACDIQEKYPDLFQVSLEVEVEPRDRPGHEAPPWEGAGMRGLNPKILFSSREEQQDVLSKFGKPELPRQPGSTAQYDAEAGPADAEPPESDSPHSSVDADHFLHTLDWREDRDSSVGPEGHLAEEGPSAVAEDGCVSEPSDEEAPTPSAESGDTGDEDVPAPAAPQDDSVDLLGLHAEAGPVPAPQAPGGPPSNADLLGCLLGAPGPAPEGAVGGLLDGDAALLFSSPAPAPCTQTTAPAADPFGPLLLPPDVDTPPSSQPDLFGEFLNSDAPAAQPAPFPSTHSAPPPACSTDFLHLGDLPAEPSKMTASASHPDLLGAWDAWAEAPAPALAADGTFFSPGDQPAPSGLPAGQTKSQSQDPFADLGDLSAGLQGSPAAAAPGGFGPKAAPPPKASSSWQTSRPPAQGTPWPPQAKPPPKASVPPRPNYTSSFSVIGSREERGVRAPSFGPKPRVSENDFEDLLSNQGFSSRADRKGPRTIAEMRRQDQARDLDPLKLKLLEWTEGKERNIRALLSTLHTALWDGESRWTPVGMADLVTPAQVKKHYRRAVLVVHPDKARGQPYEQYARMIFMELNDAWAEFESQGSRPLF

Target ORF information:

RefSeq Version	XM_005208283.1
Organism	Bos taurus(cattle)
Definition	Bos taurus cyclin G associated kinase (GAK), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005208283.1

ORF Insert Sequence:

ATGGAGCTCT GCAGAGGGCA GCTGGTGGAA TTTTTAAAAA AAGTTGAATC GAAAGGTCCG 
CTCTCGTGCG ACACCGTCCT GAAGATCTTC TACCAGACGT GCCGGGCGGT GCAGCACATG 
CACAGACAGA AGCCGCCCAT CATCCACAGG GACCTCAAGG TTGAAAACTT ACTGCTTAGT 
AACCAGGGGA CCATCAAGCT GTGCGACTTC GGCAGTGCCA CGACCATCTC ACACTACCCA 
GACTACAGCT GGAGCGCCCA GCGCCGGGCC CTGGTGGAGG AGGAGATCAC CAGGAACACG 
ACCCCCATGT ACAGGACGCC GGAAATTGTG GACTTGTACT CAAACTTTCC GATTGGCGAG 
AAGCAGGACA TCTGGGCCCT GGGCTGCATC CTGTACCTGC TCTGCTTCGG GCAGCACCCC 
TTTGAGGACG GAGCGAAGCT TCGCATCGTC AACGGGAAGT ACGCAATCCC TGCCGACGAC 
ACGCGGTACT CCGTGTTCCA CAGCCTCATC CGTTCCATGC TCAAGGTCAA CCCGGAGGAG 
CGGCTGTCCG TCACCGAGCT GGTCAACCAG CTCCAGGAGA TCGCGGCCGC CCGCAACGTG 
AACCCCAAGT CACCCATCAC TGAGCTCCTG GAGCAGAATG GAGGCTATGG GCACGCGGCC 
CCTCCCGGCT CCACGGGCAG CAGTCTGGTG GTCACTGAGT ACGAGCAGCC CTATGGCGGG 
TTCCTGGACA TTCTGCGTGG GGGCACCGAG CGCCTCTTCA CCAACATCAA GGACACCTCC 
TCCAAAGTCA TCCAGTCCGT TGCCAACTAT GCGAAGGGAG ATCTGGATAT TTCTTACATC 
ACGTCTAGAA TCGCAGTCAT GTCGTTCCCG GCGGAGGGCG TGGAGTCGGC CATCAAGAAC 
AACATTGAGG ATGTACGGCT GTTCCTGGAC TCGAAGCACC CGGGCCGCTA CGCCGTCTAC 
AACCTGTCCC CGCGGACCTA CCGGCCCTCG AAGTTCCACA ACCGGGTCTC CGACTGCGGC 
TGGGTGGCCC GGCGGGCCCC GAGCCTCCGC AGCCTGTACA GCCTCTGCAG GAACATGCAC 
GCCTGGCTGC GCCGCGACCC TGGCAACGTC TGCGTGGTGC ACTGCATGGA CGGGAGGGCG 
GTGTCGGCCG TGGCCGTGTG CTCCTTCTTG TGCTTCTGCC GGCTCTTCAG CACCGCGGAG 
GCCGCTGTGT ACATGTTCAG CATGAAGCGG TGCCCACCGG GCATCTGGCC ATCCCACAAG 
AGGTACATTG AGTACATGTG TGACATGGTG GCCGAGGAGC CCGTCACGCC CCACAGCAAG 
CCCATCCTGG TGAAGGCCGT GGTCATGACG CCTGTGCCGC TCTTCAGCAA GCAGAGGAGC 
GGCTGCAGGC CCTTCTGCGA GGTCTACGTG GGGGATGAGC GCGTGACCAC CACGTCCCAG 
GAATACGACA AGATGAGAGA CTTTAAAATT GAGGATGGCA AAGCGGTCAT TCCCCTGGGC 
ATAACAGTCC AGGGGGACGT GCTCATCATC ATCTATCATG CGAGGTCCAC CCTGGGAGGA 
AGGCTGCAGG CCAAGATGGC GTCCATGAAG ATGTTTCAGA TCCAGTTTCA CACAGGGTTC 
GTGCCTCGGA ACGCAGCTAC TGTGAAGTTT GCCAAGTATG ATCTGGATGC TTGCGACATC 
CAGGAGAAGT ACCCCGACTT GTTCCAAGTG AGCCTGGAGG TGGAGGTGGA GCCCAGAGAC 
AGGCCCGGCC ACGAGGCCCC GCCCTGGGAG GGTGCAGGCA TGCGAGGGCT GAACCCCAAG 
ATCCTCTTCT CCAGCCGGGA GGAGCAGCAA GATGTCCTGT CCAAGTTTGG GAAGCCAGAG 
CTCCCCAGGC AGCCTGGGTC CACCGCACAG TACGACGCCG AGGCCGGGCC CGCGGATGCC 
GAGCCCCCCG AGTCAGACTC GCCGCACAGC AGCGTGGATG CTGACCACTT CCTGCACACG 
CTGGACTGGC GGGAGGATCG GGACTCCAGT GTGGGCCCGG AGGGTCACCT GGCCGAGGAG 
GGTCCCTCTG CTGTGGCCGA GGACGGCTGT GTGAGTGAGC CATCGGACGA GGAAGCCCCG 
ACACCCTCTG CGGAGAGCGG GGACACAGGC GATGAGGACG TGCCCGCCCC CGCCGCCCCG 
CAGGACGACA GCGTGGACCT TCTGGGGCTG CACGCGGAGG CGGGGCCTGT GCCCGCCCCC 
CAGGCCCCCG GCGGCCCCCC CAGCAATGCA GACCTGCTCG GCTGCCTGCT CGGGGCCCCG 
GGCCCCGCTC CGGAGGGTGC TGTGGGGGGC CTGCTCGACG GGGATGCGGC CCTGCTCTTC 
AGCAGCCCAG CCCCCGCGCC TTGCACCCAG ACAACTGCCC CGGCCGCTGA CCCGTTCGGC 
CCGCTCCTGC TGCCGCCCGA CGTGGACACC CCTCCTAGCT CGCAGCCTGA CCTCTTCGGG 
GAATTCCTCA ACTCAGACGC CCCGGCCGCC CAGCCCGCGC CCTTCCCCTC CACCCACAGC 
GCTCCGCCGC CAGCCTGCAG CACCGACTTC CTGCATCTTG GGGACCTGCC CGCCGAGCCC 
AGCAAGATGA CAGCCTCTGC CAGCCACCCC GACCTGCTGG GAGCCTGGGA TGCCTGGGCT 
GAGGCGCCGG CCCCCGCGCT GGCTGCAGAC GGCACCTTCT TCTCTCCTGG AGACCAGCCA 
GCCCCTTCTG GCCTTCCGGC TGGCCAGACC AAGTCTCAGA GCCAGGACCC GTTTGCAGAC 
CTTGGCGACC TCAGTGCCGG CCTCCAAGGC TCGCCTGCCG CAGCTGCTCC TGGGGGCTTT 
GGTCCCAAAG CAGCGCCCCC TCCCAAGGCC AGCAGCTCCT GGCAGACGAG TCGGCCCCCA 
GCCCAGGGCA CCCCGTGGCC CCCCCAGGCC AAGCCGCCCC CCAAAGCCAG TGTACCGCCA 
AGGCCTAACT ACACCTCCAG CTTCAGTGTG ATCGGTAGCC GGGAGGAGCG GGGGGTCCGC 
GCCCCCAGCT TTGGTCCAAA GCCGAGGGTG TCAGAAAACG ACTTTGAAGA CCTGCTCTCC 
AACCAGGGCT TCTCCTCGCG GGCTGACAGG AAGGGGCCGC GGACCATCGC TGAGATGAGG 
AGGCAGGACC AGGCCCGGGA CTTGGATCCG CTGAAGCTGA AGCTTCTGGA GTGGACGGAG 
GGCAAGGAGA GGAACATCCG CGCCCTGCTG TCCACGCTGC ACACGGCGCT GTGGGACGGC 
GAGAGCCGCT GGACGCCGGT GGGCATGGCC GACCTGGTGA CGCCCGCACA AGTGAAGAAG 
CACTACCGCC GTGCCGTGCT GGTGGTCCAC CCCGACAAGG CCCGGGGGCA GCCCTACGAG 
CAGTATGCCC GGATGATCTT CATGGAGCTC AACGATGCGT GGGCTGAGTT TGAGAGCCAG 
GGCTCCCGGC CACTCTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAK ( XM_010806359.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010804661.1 Bos taurus cyclin G associated kinase (GAK), transcript variant X4, mRNA.

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CloneID	OBa47972
Clone ID Related Accession (Same CDS sequence)	XM_010806359.2
Accession Version	XM_010806359.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3417bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	cyclin-G-associated kinase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103986.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010806359.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCTGC TGCAGTCCGC GCTCGACTTC CTGGCGGGCC CGGGCTCCCT GGGGGGCGCG 
GCCGGCCGCG ACCAGACGGA CTTCGTGGGG CAGACGGTGG AGATGGGCGA GCTGCGGCTG 
CGGGTGCGCC GGGTCCTGGC TGAAGGAGGG TTTGCATTTG TGTATGAAGC TCAAGATCTG 
GGAAGTGGCA GAGAGTATGC ATTGAAGAGA CTGCTGTCCA ACGAAGAGGA GAAGAACAGA 
GCCATCATTC AGGAAGTGTG TTTCATGAAA AAACTTTCAG GCCACCCAAA CATTGTCCAG 
TTTTGTTCTG CAGCATCGAT AGGAAAAGAG GAGTCGGACA CTGGGCAGGC GGAGTTCCTG 
CTGCTCATGG AGCTCTGCAG AGGGCAGCTG GTGGAATTTT TAAAAAAAGT TGAATCGAAA 
GGTCCGCTCT CGTGCGACAC CGTCCTGAAG ATCTTCTACC AGACGTGCCG GGCGGTGCAG 
CACATGCACA GACAGAAGCC GCCCATCATC CACAGGGACC TCAAGGTTGA AAACTTACTG 
CTTAGTAACC AGGGGACCAT CAAGCTGTGC GACTTCGGCA GTGCCACGAC CATCTCACAC 
TACCCAGACT ACAGCTGGAG CGCCCAGCGC CGGGCCCTGG TGGAGGAGGA GATCACCAGG 
AACACGACCC CCATGTACAG GACGCCGGAA ATTGTGGACT TGTACTCAAA CTTTCCGATT 
GGCGAGAAGC AGGACATCTG GGCCCTGGGC TGCATCCTGT ACCTGCTCTG CTTCGGGCAG 
CACCCCTTTG AGGACGGAGC GAAGCTTCGC ATCGTCAACG GGAAGTACGC AATCCCTGCC 
GACGACACGC GGTACTCCGT GTTCCACAGC CTCATCCGTT CCATGCTCAA GGTCAACCCG 
GAGGAGCGGC TGTCCGTCAC CGAGCTGGTC AACCAGCTCC AGGAGATCGC GGCCGCCCGC 
AACGTGAACC CCAAGTCACC CATCACTGAG CTCCTGGAGC AGAATGGAGG CTATGGGCAC 
GCGGCCCCTC CCGGCTCCAC GGGCAGCAGT CTGGTGGTCA CTGAGTACGA GCAGCCCTAT 
GGCGGGTTCC TGGACATTCT GCGTGGGGGC ACCGAGCGCC TCTTCACCAA CATCAAGGAC 
ACCTCCTCCA AAGTCATCCA GTCCGTTGCC AACTATGCGA AGGGAGATCT GGATATTTCT 
TACATCACGT CTAGAATCGC AGTCATGTCG TTCCCGGCGG AGGGCGTGGA GTCGGCCATC 
AAGAACAACA TTGAGGATGT ACGGCTGTTC CTGGACTCGA AGCACCCGGG CCGCTACGCC 
GTCTACAACC TGTCCCCGCG GACCTACCGG CCCTCGAAGT TCCACAACCG GGTCTCCGAC 
TGCGGCTGGG TGGCCCGGCG GGCCCCGAGC CTCCGCAGCC TGTACAGCCT CTGCAGGAAC 
ATGCACGCCT GGCTGCGCCG CGACCCTGGC AACGTCTGCG TGGTGCACTG CATGGACGGG 
AGGGCGGTGT CGGCCGTGGC CGTGTGCTCC TTCTTGTGCT TCTGCCGGCT CTTCAGCACC 
GCGGAGGCCG CTGTGTACAT GTTCAGCATG AAGCGGTGCC CACCGGGCAT CTGGCCATCC 
CACAAGAGGT ACATTGAGTA CATGTGTGAC ATGGTGGCCG AGGAGCCCGT CACGCCCCAC 
AGCAAGCCCA TCCTGGTGAA GGCCGTGGTC ATGACGCCTG TGCCGCTCTT CAGCAAGCAG 
AGGAGCGGCT GCAGGCCCTT CTGCGAGGTC TACGTGGGGG ATGAGCGCGT GACCACCACG 
TCCCAGGAAT ACGACAAGAT GAGAGACTTT AAAATTGAGG ATGGCAAAGC GGTCATTCCC 
CTGGGCATAA CAGTCCAGGG GGACGTGCTC ATCATCATCT ATCATGCGAG GTCCACCCTG 
GGAGGAAGGC TGCAGGCCAA GATGGCGTCC ATGAAGATGT TTCAGATCCA GTTTCACACA 
GGGTTCGTGC CTCGGAACGC AGCTACTGTG AAGTTTGCCA AGTATGATCT GGATGCTTGC 
GACATCCAGG AGAAGTACCC CGACTTGTTC CAAGTGAGCC TGGAGGTGGA GGTGGAGCCC 
AGAGACAGGC CCGGCCACGA GGCCCCGCCC TGGGAGGGTG CAGGCATGCG AGGGCTGAAC 
CCCAAGATCC TCTTCTCCAG CCGGGAGGAG CAGCAAGATG TCCTGTCCAA GTTTGGGAAG 
CCAGAGCTCC CCAGGCAGCC TGGGTCCACC GCACAGTACG ACGCCGAGGC CGGGCCCGCG 
GATGCCGAGC CCCCCGAGTC AGACTCGCCG CACAGCAGCG TGGATGCTGA CCACTTCCTG 
CACACGCTGG ACTGGCGGGA GGATCGGGAC TCCAGTGTGG GCCCGGAGGG TCACCTGGCC 
GAGGAGGGTC CCTCTGCTGT GGCCGAGGAC GGCTGTGTGA GTGAGCCATC GGACGAGGAA 
GCCCCGACAC CCTCTGCGGA GAGCGGGGAC ACAGGCGATG AGGACGTGCC CGCCCCCGCC 
GCCCCGCAGG ACGACAGCGT GGACCTTCTG GGGCTGCACG CGGAGGCGGG GCCTGTGCCC 
GCCCCCCAGG CCCCCGGCGG CCCCCCCAGC AATGCAGACC TGCTCGGCTG CCTGCTCGGG 
GCCCCGGGCC CCGCTCCGGA GGGTGCTGTG GGGGGCCTGC TCGACGGGGA TGCGGCCCTG 
CTCTTCAGCA GCCCAGCCCC CGCGCCTTGC ACCCAGACAA CTGCCCCGGC CGCTGACCCG 
TTCGGCCCGC TCCTGCTGCC GCCCGACGTG GACACCCCTC CTAGCTCGCA GCCTGACCTC 
TTCGGGGAAT TCCTCAACTC AGACGCCCCG GCCGCCCAGC CCGCGCCCTT CCCCTCCACC 
CACAGCGCTC CGCCGCCAGC CTGCAGCACC GACTTCCTGC ATCTTGGGGA CCTGCCCGCC 
GAGCCCAGCA AGATGACAGC CTCTGCCAGC CACCCCGACC TGCTGGGAGC CTGGGATGCC 
TGGGCTGAGG CGCCGGCCCC CGCGCTGGCT GCAGACGGCA CCTTCTTCTC TCCTGGAGAC 
CAGCCAGCCC CTTCTGGCCT TCCGGCTGGC CAGACCAAGT CTCAGAGCCA GGACCCGTTT 
GCAGACCTTG GCGACCTCAG TGCCGGCCTC CAAGGGCCCA GCAACAGCAG CAGATGCCCT 
TCTGCCTTCT CTCAGGAGCT TGTTGCCAAG ACAACGCCAG GAGCTGCCAG AGCAGGCACA 
GGCACTGGCT CCTCTGCCAG CTCCATGGAG TGTGGGGGCC AGCTGGGGGC TGCCTCAGCC 
CAGGATGGTG GAACCTGCCT GCCCCGCTTG GAGTGTGGGG CCGGGGTGCA GAATTCACCA 
CCATCACTGT CCTTTCTGAG AAGCCCTCTA AGGACGTTGG TTCTTTGGAG GCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010804661.1
CDS	114..3530
Translation	MSLLQSALDFLAGPGSLGGAAGRDQTDFVGQTVEMGELRLRVRRVLAEGGFAFVYEAQDLGSGREYALKRLLSNEEEKNRAIIQEVCFMKKLSGHPNIVQFCSAASIGKEESDTGQAEFLLLMELCRGQLVEFLKKVESKGPLSCDTVLKIFYQTCRAVQHMHRQKPPIIHRDLKVENLLLSNQGTIKLCDFGSATTISHYPDYSWSAQRRALVEEEITRNTTPMYRTPEIVDLYSNFPIGEKQDIWALGCILYLLCFGQHPFEDGAKLRIVNGKYAIPADDTRYSVFHSLIRSMLKVNPEERLSVTELVNQLQEIAAARNVNPKSPITELLEQNGGYGHAAPPGSTGSSLVVTEYEQPYGGFLDILRGGTERLFTNIKDTSSKVIQSVANYAKGDLDISYITSRIAVMSFPAEGVESAIKNNIEDVRLFLDSKHPGRYAVYNLSPRTYRPSKFHNRVSDCGWVARRAPSLRSLYSLCRNMHAWLRRDPGNVCVVHCMDGRAVSAVAVCSFLCFCRLFSTAEAAVYMFSMKRCPPGIWPSHKRYIEYMCDMVAEEPVTPHSKPILVKAVVMTPVPLFSKQRSGCRPFCEVYVGDERVTTTSQEYDKMRDFKIEDGKAVIPLGITVQGDVLIIIYHARSTLGGRLQAKMASMKMFQIQFHTGFVPRNAATVKFAKYDLDACDIQEKYPDLFQVSLEVEVEPRDRPGHEAPPWEGAGMRGLNPKILFSSREEQQDVLSKFGKPELPRQPGSTAQYDAEAGPADAEPPESDSPHSSVDADHFLHTLDWREDRDSSVGPEGHLAEEGPSAVAEDGCVSEPSDEEAPTPSAESGDTGDEDVPAPAAPQDDSVDLLGLHAEAGPVPAPQAPGGPPSNADLLGCLLGAPGPAPEGAVGGLLDGDAALLFSSPAPAPCTQTTAPAADPFGPLLLPPDVDTPPSSQPDLFGEFLNSDAPAAQPAPFPSTHSAPPPACSTDFLHLGDLPAEPSKMTASASHPDLLGAWDAWAEAPAPALAADGTFFSPGDQPAPSGLPAGQTKSQSQDPFADLGDLSAGLQGPSNSSRCPSAFSQELVAKTTPGAARAGTGTGSSASSMECGGQLGAASAQDGGTCLPRLECGAGVQNSPPSLSFLRSPLRTLVLWRP

Target ORF information:

RefSeq Version	XM_010806359.2
Organism	Bos taurus(cattle)
Definition	Bos taurus cyclin G associated kinase (GAK), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010806359.2

ORF Insert Sequence:

ATGTCGCTGC TGCAGTCCGC GCTCGACTTC CTGGCGGGCC CGGGCTCCCT GGGGGGCGCG 
GCCGGCCGCG ACCAGACGGA CTTCGTGGGG CAGACGGTGG AGATGGGCGA GCTGCGGCTG 
CGGGTGCGCC GGGTCCTGGC TGAAGGAGGG TTTGCATTTG TGTATGAAGC TCAAGATCTG 
GGAAGTGGCA GAGAGTATGC ATTGAAGAGA CTGCTGTCCA ACGAAGAGGA GAAGAACAGA 
GCCATCATTC AGGAAGTGTG TTTCATGAAA AAACTTTCAG GCCACCCAAA CATTGTCCAG 
TTTTGTTCTG CAGCATCGAT AGGAAAAGAG GAGTCGGACA CTGGGCAGGC GGAGTTCCTG 
CTGCTCATGG AGCTCTGCAG AGGGCAGCTG GTGGAATTTT TAAAAAAAGT TGAATCGAAA 
GGTCCGCTCT CGTGCGACAC CGTCCTGAAG ATCTTCTACC AGACGTGCCG GGCGGTGCAG 
CACATGCACA GACAGAAGCC GCCCATCATC CACAGGGACC TCAAGGTTGA AAACTTACTG 
CTTAGTAACC AGGGGACCAT CAAGCTGTGC GACTTCGGCA GTGCCACGAC CATCTCACAC 
TACCCAGACT ACAGCTGGAG CGCCCAGCGC CGGGCCCTGG TGGAGGAGGA GATCACCAGG 
AACACGACCC CCATGTACAG GACGCCGGAA ATTGTGGACT TGTACTCAAA CTTTCCGATT 
GGCGAGAAGC AGGACATCTG GGCCCTGGGC TGCATCCTGT ACCTGCTCTG CTTCGGGCAG 
CACCCCTTTG AGGACGGAGC GAAGCTTCGC ATCGTCAACG GGAAGTACGC AATCCCTGCC 
GACGACACGC GGTACTCCGT GTTCCACAGC CTCATCCGTT CCATGCTCAA GGTCAACCCG 
GAGGAGCGGC TGTCCGTCAC CGAGCTGGTC AACCAGCTCC AGGAGATCGC GGCCGCCCGC 
AACGTGAACC CCAAGTCACC CATCACTGAG CTCCTGGAGC AGAATGGAGG CTATGGGCAC 
GCGGCCCCTC CCGGCTCCAC GGGCAGCAGT CTGGTGGTCA CTGAGTACGA GCAGCCCTAT 
GGCGGGTTCC TGGACATTCT GCGTGGGGGC ACCGAGCGCC TCTTCACCAA CATCAAGGAC 
ACCTCCTCCA AAGTCATCCA GTCCGTTGCC AACTATGCGA AGGGAGATCT GGATATTTCT 
TACATCACGT CTAGAATCGC AGTCATGTCG TTCCCGGCGG AGGGCGTGGA GTCGGCCATC 
AAGAACAACA TTGAGGATGT ACGGCTGTTC CTGGACTCGA AGCACCCGGG CCGCTACGCC 
GTCTACAACC TGTCCCCGCG GACCTACCGG CCCTCGAAGT TCCACAACCG GGTCTCCGAC 
TGCGGCTGGG TGGCCCGGCG GGCCCCGAGC CTCCGCAGCC TGTACAGCCT CTGCAGGAAC 
ATGCACGCCT GGCTGCGCCG CGACCCTGGC AACGTCTGCG TGGTGCACTG CATGGACGGG 
AGGGCGGTGT CGGCCGTGGC CGTGTGCTCC TTCTTGTGCT TCTGCCGGCT CTTCAGCACC 
GCGGAGGCCG CTGTGTACAT GTTCAGCATG AAGCGGTGCC CACCGGGCAT CTGGCCATCC 
CACAAGAGGT ACATTGAGTA CATGTGTGAC ATGGTGGCCG AGGAGCCCGT CACGCCCCAC 
AGCAAGCCCA TCCTGGTGAA GGCCGTGGTC ATGACGCCTG TGCCGCTCTT CAGCAAGCAG 
AGGAGCGGCT GCAGGCCCTT CTGCGAGGTC TACGTGGGGG ATGAGCGCGT GACCACCACG 
TCCCAGGAAT ACGACAAGAT GAGAGACTTT AAAATTGAGG ATGGCAAAGC GGTCATTCCC 
CTGGGCATAA CAGTCCAGGG GGACGTGCTC ATCATCATCT ATCATGCGAG GTCCACCCTG 
GGAGGAAGGC TGCAGGCCAA GATGGCGTCC ATGAAGATGT TTCAGATCCA GTTTCACACA 
GGGTTCGTGC CTCGGAACGC AGCTACTGTG AAGTTTGCCA AGTATGATCT GGATGCTTGC 
GACATCCAGG AGAAGTACCC CGACTTGTTC CAAGTGAGCC TGGAGGTGGA GGTGGAGCCC 
AGAGACAGGC CCGGCCACGA GGCCCCGCCC TGGGAGGGTG CAGGCATGCG AGGGCTGAAC 
CCCAAGATCC TCTTCTCCAG CCGGGAGGAG CAGCAAGATG TCCTGTCCAA GTTTGGGAAG 
CCAGAGCTCC CCAGGCAGCC TGGGTCCACC GCACAGTACG ACGCCGAGGC CGGGCCCGCG 
GATGCCGAGC CCCCCGAGTC AGACTCGCCG CACAGCAGCG TGGATGCTGA CCACTTCCTG 
CACACGCTGG ACTGGCGGGA GGATCGGGAC TCCAGTGTGG GCCCGGAGGG TCACCTGGCC 
GAGGAGGGTC CCTCTGCTGT GGCCGAGGAC GGCTGTGTGA GTGAGCCATC GGACGAGGAA 
GCCCCGACAC CCTCTGCGGA GAGCGGGGAC ACAGGCGATG AGGACGTGCC CGCCCCCGCC 
GCCCCGCAGG ACGACAGCGT GGACCTTCTG GGGCTGCACG CGGAGGCGGG GCCTGTGCCC 
GCCCCCCAGG CCCCCGGCGG CCCCCCCAGC AATGCAGACC TGCTCGGCTG CCTGCTCGGG 
GCCCCGGGCC CCGCTCCGGA GGGTGCTGTG GGGGGCCTGC TCGACGGGGA TGCGGCCCTG 
CTCTTCAGCA GCCCAGCCCC CGCGCCTTGC ACCCAGACAA CTGCCCCGGC CGCTGACCCG 
TTCGGCCCGC TCCTGCTGCC GCCCGACGTG GACACCCCTC CTAGCTCGCA GCCTGACCTC 
TTCGGGGAAT TCCTCAACTC AGACGCCCCG GCCGCCCAGC CCGCGCCCTT CCCCTCCACC 
CACAGCGCTC CGCCGCCAGC CTGCAGCACC GACTTCCTGC ATCTTGGGGA CCTGCCCGCC 
GAGCCCAGCA AGATGACAGC CTCTGCCAGC CACCCCGACC TGCTGGGAGC CTGGGATGCC 
TGGGCTGAGG CGCCGGCCCC CGCGCTGGCT GCAGACGGCA CCTTCTTCTC TCCTGGAGAC 
CAGCCAGCCC CTTCTGGCCT TCCGGCTGGC CAGACCAAGT CTCAGAGCCA GGACCCGTTT 
GCAGACCTTG GCGACCTCAG TGCCGGCCTC CAAGGGCCCA GCAACAGCAG CAGATGCCCT 
TCTGCCTTCT CTCAGGAGCT TGTTGCCAAG ACAACGCCAG GAGCTGCCAG AGCAGGCACA 
GGCACTGGCT CCTCTGCCAG CTCCATGGAG TGTGGGGGCC AGCTGGGGGC TGCCTCAGCC 
CAGGATGGTG GAACCTGCCT GCCCCGCTTG GAGTGTGGGG CCGGGGTGCA GAATTCACCA 
CCATCACTGT CCTTTCTGAG AAGCCCTCTA AGGACGTTGG TTCTTTGGAG GCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	GAK
Entrez Gene ID	511296
Full Name	cyclin G associated kinase
General protein information	Preferred Names cyclin G associated kinase Names cyclin-G-associated kinase
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Rattus norvegicus (Norway rat)	Gak	NP_112292.1
Homo sapiens (human)	GAK	NP_005246.2
Bos taurus (cattle)	GAK	NP_001039549.1
Danio rerio (zebrafish)	gak	XP_003198642.2
Pan troglodytes (chimpanzee)	GAK	XP_001140835.1
Mus musculus (house mouse)	Gak	NP_705797.1
Gallus gallus (chicken)	GAK	XP_424873.4
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003715	XP_310244.7
	GAK	XP_001094120.2
Canis lupus familiaris (dog)	GAK	XP_536279.4
Drosophila melanogaster (fruit fly)	aux	NP_001262243.1
Danio rerio (zebrafish)	LOC100151158	XP_001919224.1
Xenopus tropicalis (tropical clawed frog)	gak	NP_001016350.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

GAK cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

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Target ORF information:

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Target ORF information:

Target ORF information:

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ORF Insert Sequence:

Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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Target ORF information:

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ORF Insert Sequence:

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