GAK cDNA ORF clone, Bos taurus(cattle)

The following GAK gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GAK cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa235256 XM_024993151.1
Latest version!
Bos taurus cyclin G associated kinase (GAK), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa235257 XM_010806358.3
Latest version!
Bos taurus cyclin G associated kinase (GAK), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa235258 XM_024993153.1
Latest version!
Bos taurus cyclin G associated kinase (GAK), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa235258 XM_024993152.1
Latest version!
Bos taurus cyclin G associated kinase (GAK), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa235258 XM_005208283.2
Latest version!
Bos taurus cyclin G associated kinase (GAK), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa235259 XM_010806359.3
Latest version!
Bos taurus cyclin G associated kinase (GAK), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa40735 NM_001046084.2
Latest version!
Bos taurus cyclin G associated kinase (GAK), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa20662 XM_010806358.2
Latest version!
Bos taurus cyclin G associated kinase (GAK), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa20663 XM_005208283.1
Latest version!
Bos taurus cyclin G associated kinase (GAK), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OBa20663 XM_015471751.1
Latest version!
Bos taurus cyclin G associated kinase (GAK), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OBa47972 XM_010806359.2
Latest version!
Bos taurus cyclin G associated kinase (GAK), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa235256
    Clone ID Related Accession (Same CDS sequence) XM_024993151.1
    Accession Version XM_024993151.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3663bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product cyclin-G-associated kinase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGTCGCTGC TGCAGTCCGC GCTCGACTTC CTGGCGGGCC CGGGCTCCCT GGGGGGCGCG 
    GCCGGCCGCG ACCAGACGGA CTTCGTGGGG CAGACGGTGG AGATGGGCGA GCTGCGGCTG
    CGGGTGCGCC GGGTCCTGGC TGAAGGAGGG TTTGCATTTG TGTATGAAGC TCAAGATCTG
    GGAAGTGGCA GAGAGTATGC ATTGAAGAGA CTGCTGTCCA ACGAAGAGGA GAAGAACAGA
    GCCATCATTC AGGAAGTGTG TTTCATGAAA AAACTTTCAG GCCACCCAAA CATTGTCCAG
    TTTTGTTCTG CAGCATCGAT AGGAAAAGAG GAGTCGGACA CTGGGCAGGC GGAGTTCCTG
    CTGCTCATGG AGCTCTGCAG AGGGCAGCTG GTGGAATTTT TAAAAAAAGT TGAATCGAAA
    GGTCCGCTCT CGTGCGACAC CGTCCTGAAG ATCTTCTACC AGACGTGCCG GGCGGTGCAG
    CACATGCACA GACAGAAGCC GCCCATCATC CACAGGGACC TCAAGGTTGA AAACTTACTG
    CTTAGTAACC AGGGGACCAT CAAGCTGTGT GACTTCGGCA GTGCCACGAC CATCTCACAC
    TACCCAGACT ACAGCTGGAG CGCCCAGCGC CGGGCCCTGG TGGAGGAGGA GATCACCAGG
    AACACGACCC CCATGTACAG GACGCCGGAA ATTGTGGACT TGTACTCAAA CTTTCCGATT
    GGCGAGAAGC AGGACATCTG GGCCCTGGGC TGCATCCTGT ACCTGCTCTG CTTCGGGCAG
    CACCCCTTTG AGGACGGAGC GAAGCTTCGC ATCGTCAACG GGAAGTACGC AATCCCTGCC
    GACGACACGC GGTACTCCGT GTTCCACAGC CTCATCCGTT CCATGCTCAA GGTCAACCCG
    GAGGAGCGGC TGTCCGTCAC CGAGCTGGTC AACCAGCTCC AGGAGATCGC GGCCGCCCGC
    AACGTGAACC CCAAGTCACC CATCACTGAG CTCCTGGAGC AGAATGGAGG CTATGGGCAC
    GCGGCCCCTC CCGGCTCCAC GGGCAGCAGT CTGGTGGTCA CTGAGTACGA GCAGCCCTAT
    GGCGGGTTCC TGGACATTCT GCGTGGGGGC ACCGAGCGCC TCTTCACCAA CATCAAGGAC
    ACCTCCTCCA AAGTCATCCA GTCCGTTGCC AACTATGCGA AGGGAGATCT GGATATTTCT
    TACATCACGT CTAGAATCGC AGTCATGTCG TTCCCGGCGG AGGGCGTGGA GTCGGCCATC
    AAGAACAACA TTGAGGATGT ACGGCTGTTC CTGGACTCGA AGCACCCGGG CCGCTACGCC
    GTCTACAACC TGTCCCCGCG GACCTACCGG CCCTCGAAGT TCCACAACCG GGTCTCCGAC
    TGCGGCTGGG TGGCCCGGCG GGCCCCGAGC CTCCGCAGCC TGTACAGCCT CTGCAGGAAC
    ATGCACGCCT GGCTGCGCCG CGACCCTGGC AACGTCTGCG TGGTGCACTG CATGGACGGG
    AGGGCGGTGT CGGCCGTGGC CGTGTGCTCC TTCTTGTGCT TCTGCCGGCT CTTCAGCACC
    GCGGAGGCCG CTGTGTACAT GTTCAGCATG AAGCGGTGCC CACCGGGCAT CTGGCCATCC
    CACAAGAGGT ACATTGAGTA CATGTGTGAC ATGGTGGCCG AGGAGCCCGT CACGCCCCAC
    AGCAAGCCCA TCCTGGTGAA GGCCGTGGTC ATGACGCCTG TGCCGCTCTT CAGCAAGCAG
    AGGAGCGGCT GCAGGCCCTT CTGCGAGGTC TACGTGGGGG ATGAGCGCGT GACCACCACG
    TCCCAGGAAT ACGACAAGAT GAGAGACTTT AAAATTGAGG ATGGCAAAGC GGTCATTCCC
    CTGGGCATAA CAGTCCAGGG GGACGTGCTC ATCATCATCT ATCATGCGAG GTCCACCCTG
    GGAGGAAGGC TGCAGGCCAA GATGGCGTCC ATGAAGATGT TTCAGATCCA GTTTCACACA
    GGGTTCGTGC CTCGGAACGC AGCTACTGTG AAGTTTGCCA AGTATGATCT GGATGCTTGC
    GACATCCAGG AGAAGTACCC CGACTTGTTC CAAGTGAGCC TGGAGGTGGA GGTGGAGCCC
    AGAGACAGGC CCGGCCACGA GGCCCCGCCC TGGGAGGGTG CAGGCATGCG AGGGCTGAAC
    CCCAAGATCC TCTTCTCCAG CCGGGAGGAG CAGCAAGATG TCCTGTCCAA GTTTGAGGAT
    CGGGACTCCA GTGTGGGCCC GGAGGGTCAC CTGGCCGAGG AGGGTCCCTC TGCTGTGGCC
    GAGGACGGCT GTGTGAGTGA GCCATCGGAC GAGGAAGCCC CGACACCCTC TGCGGAGAGC
    GGGGACACAG GCGATGAGGA CGTGCCCGCC CCCGCCGCCC CGCAGGACGA CAGCGTGGAC
    CTTCTGGGGC TGCACGCGGA GGCGGGGCCT GTGCCCGCCC CCCAGGCCCC CGGCGGCCCC
    CCCAGCAATG CAGACCTGCT CGGCTGCCTG CTCGGGGCCC CGGGCCCCGC TCCGGAGGGT
    GCTGTGGGGG GCCTGCTCGA CGGGGATGCG GCCCTGGTCT TCAGCAGCCC AGCCCCCGCG
    CCTTGCACCC AGACAACTGC CCCGGCCGCT GACCCGTTCG GCCCGCTCCT GCTGCCGCCC
    GACGTGGACA CCCCTCCTAG CTCGCAGCCT GACCTCTTCG GGGAATTCCT CAACTCAGAC
    GCCCCGGCCG CCCAGCCCGC GCCCTTCCCC TCCACCCACA GCGCTCCGCC GCCAGCCTGC
    AGCACCGACT TCCTGCATCT TGGGGACCTG CCCGCCGAGC CCAGCAAGAT GACAGCCTCT
    GCCAGCCACC CCGACCTGCT GGGAGCCTGG GATGCCTGGG CTGAGGCGCC GGCCCCCGCG
    CTGGCTGCAG ACGGCACCTT CTTCTCTCCT GGAGACCAGC CAGCCCCTTC TGGCCTTCCG
    GCTGGCCAGA CCAAGTCTCA GAGCCAGGAC CCGTTTGCAG ACCTTGGCGA CCTCAGTGCC
    GGCCTCCAAG GCTCGCCTGC CGCAGCTGCT CCTGGGGGCT TTGGTCCCAA AGCAGCGCCC
    CCTCCCAAGG CCAGCAGCTC CTGGCAGACG AGTCGGCCCC CAGCCCAGGG CACCCCGTGG
    CCCCCCCAGG CCAAGCCGCC CCCCAAAGCC AGTGTACCGC CAAGGCCTAA CTACACCTCC
    AGCTTCAGTG TGATCGGTAG CCGGGAGGAG CGGGGGGTCC GCGCCCCCAG CTTTGGTCCA
    AAGCCGAGGG TGTCAGAGAA CGACTTTGAA GACCTGCTCT CCAACCAGGG CTTCTCCTCG
    CGGGCTGACA GGAAGGGGCC GCGGACCATC GCTGAGATGA GGAGGCAGGA CCAGGCCCGG
    GACTTGGATC CGCTGAAGCT GAAGCTTCTG GAGTGGACGG AGGGCAAGGA GAGGAACATC
    CGCGCCCTGC TGTCCACGCT GCACACGGCG CTGTGGGACG GCGAGAGCCG CTGGACGCCG
    GTGGGCATGG CCGACCTGGT GACGCCCGCA CAAGTGAAGA AGCACTACCG CCGTGCCGTG
    CTGGTGGTCC ACCCCGACAA GGCCCGGGGG CAGCCCTACG AGCAGTATGC CCGGATGATC
    TTCATGGAGC TCAACGATGC GTGGGCTGAG TTTGAGAGCC AGGGCTCCCG GCCACTCTTC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024848919.1
    CDS114..3776
    Translation

    Target ORF information:

    RefSeq Version XM_024993151.1
    Organism Bos taurus(cattle)
    Definition Bos taurus cyclin G associated kinase (GAK), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024993151.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGTCGCTGC TGCAGTCCGC GCTCGACTTC CTGGCGGGCC CGGGCTCCCT GGGGGGCGCG 
    GCCGGCCGCG ACCAGACGGA CTTCGTGGGG CAGACGGTGG AGATGGGCGA GCTGCGGCTG
    CGGGTGCGCC GGGTCCTGGC TGAAGGAGGG TTTGCATTTG TGTATGAAGC TCAAGATCTG
    GGAAGTGGCA GAGAGTATGC ATTGAAGAGA CTGCTGTCCA ACGAAGAGGA GAAGAACAGA
    GCCATCATTC AGGAAGTGTG TTTCATGAAA AAACTTTCAG GCCACCCAAA CATTGTCCAG
    TTTTGTTCTG CAGCATCGAT AGGAAAAGAG GAGTCGGACA CTGGGCAGGC GGAGTTCCTG
    CTGCTCATGG AGCTCTGCAG AGGGCAGCTG GTGGAATTTT TAAAAAAAGT TGAATCGAAA
    GGTCCGCTCT CGTGCGACAC CGTCCTGAAG ATCTTCTACC AGACGTGCCG GGCGGTGCAG
    CACATGCACA GACAGAAGCC GCCCATCATC CACAGGGACC TCAAGGTTGA AAACTTACTG
    CTTAGTAACC AGGGGACCAT CAAGCTGTGT GACTTCGGCA GTGCCACGAC CATCTCACAC
    TACCCAGACT ACAGCTGGAG CGCCCAGCGC CGGGCCCTGG TGGAGGAGGA GATCACCAGG
    AACACGACCC CCATGTACAG GACGCCGGAA ATTGTGGACT TGTACTCAAA CTTTCCGATT
    GGCGAGAAGC AGGACATCTG GGCCCTGGGC TGCATCCTGT ACCTGCTCTG CTTCGGGCAG
    CACCCCTTTG AGGACGGAGC GAAGCTTCGC ATCGTCAACG GGAAGTACGC AATCCCTGCC
    GACGACACGC GGTACTCCGT GTTCCACAGC CTCATCCGTT CCATGCTCAA GGTCAACCCG
    GAGGAGCGGC TGTCCGTCAC CGAGCTGGTC AACCAGCTCC AGGAGATCGC GGCCGCCCGC
    AACGTGAACC CCAAGTCACC CATCACTGAG CTCCTGGAGC AGAATGGAGG CTATGGGCAC
    GCGGCCCCTC CCGGCTCCAC GGGCAGCAGT CTGGTGGTCA CTGAGTACGA GCAGCCCTAT
    GGCGGGTTCC TGGACATTCT GCGTGGGGGC ACCGAGCGCC TCTTCACCAA CATCAAGGAC
    ACCTCCTCCA AAGTCATCCA GTCCGTTGCC AACTATGCGA AGGGAGATCT GGATATTTCT
    TACATCACGT CTAGAATCGC AGTCATGTCG TTCCCGGCGG AGGGCGTGGA GTCGGCCATC
    AAGAACAACA TTGAGGATGT ACGGCTGTTC CTGGACTCGA AGCACCCGGG CCGCTACGCC
    GTCTACAACC TGTCCCCGCG GACCTACCGG CCCTCGAAGT TCCACAACCG GGTCTCCGAC
    TGCGGCTGGG TGGCCCGGCG GGCCCCGAGC CTCCGCAGCC TGTACAGCCT CTGCAGGAAC
    ATGCACGCCT GGCTGCGCCG CGACCCTGGC AACGTCTGCG TGGTGCACTG CATGGACGGG
    AGGGCGGTGT CGGCCGTGGC CGTGTGCTCC TTCTTGTGCT TCTGCCGGCT CTTCAGCACC
    GCGGAGGCCG CTGTGTACAT GTTCAGCATG AAGCGGTGCC CACCGGGCAT CTGGCCATCC
    CACAAGAGGT ACATTGAGTA CATGTGTGAC ATGGTGGCCG AGGAGCCCGT CACGCCCCAC
    AGCAAGCCCA TCCTGGTGAA GGCCGTGGTC ATGACGCCTG TGCCGCTCTT CAGCAAGCAG
    AGGAGCGGCT GCAGGCCCTT CTGCGAGGTC TACGTGGGGG ATGAGCGCGT GACCACCACG
    TCCCAGGAAT ACGACAAGAT GAGAGACTTT AAAATTGAGG ATGGCAAAGC GGTCATTCCC
    CTGGGCATAA CAGTCCAGGG GGACGTGCTC ATCATCATCT ATCATGCGAG GTCCACCCTG
    GGAGGAAGGC TGCAGGCCAA GATGGCGTCC ATGAAGATGT TTCAGATCCA GTTTCACACA
    GGGTTCGTGC CTCGGAACGC AGCTACTGTG AAGTTTGCCA AGTATGATCT GGATGCTTGC
    GACATCCAGG AGAAGTACCC CGACTTGTTC CAAGTGAGCC TGGAGGTGGA GGTGGAGCCC
    AGAGACAGGC CCGGCCACGA GGCCCCGCCC TGGGAGGGTG CAGGCATGCG AGGGCTGAAC
    CCCAAGATCC TCTTCTCCAG CCGGGAGGAG CAGCAAGATG TCCTGTCCAA GTTTGAGGAT
    CGGGACTCCA GTGTGGGCCC GGAGGGTCAC CTGGCCGAGG AGGGTCCCTC TGCTGTGGCC
    GAGGACGGCT GTGTGAGTGA GCCATCGGAC GAGGAAGCCC CGACACCCTC TGCGGAGAGC
    GGGGACACAG GCGATGAGGA CGTGCCCGCC CCCGCCGCCC CGCAGGACGA CAGCGTGGAC
    CTTCTGGGGC TGCACGCGGA GGCGGGGCCT GTGCCCGCCC CCCAGGCCCC CGGCGGCCCC
    CCCAGCAATG CAGACCTGCT CGGCTGCCTG CTCGGGGCCC CGGGCCCCGC TCCGGAGGGT
    GCTGTGGGGG GCCTGCTCGA CGGGGATGCG GCCCTGGTCT TCAGCAGCCC AGCCCCCGCG
    CCTTGCACCC AGACAACTGC CCCGGCCGCT GACCCGTTCG GCCCGCTCCT GCTGCCGCCC
    GACGTGGACA CCCCTCCTAG CTCGCAGCCT GACCTCTTCG GGGAATTCCT CAACTCAGAC
    GCCCCGGCCG CCCAGCCCGC GCCCTTCCCC TCCACCCACA GCGCTCCGCC GCCAGCCTGC
    AGCACCGACT TCCTGCATCT TGGGGACCTG CCCGCCGAGC CCAGCAAGAT GACAGCCTCT
    GCCAGCCACC CCGACCTGCT GGGAGCCTGG GATGCCTGGG CTGAGGCGCC GGCCCCCGCG
    CTGGCTGCAG ACGGCACCTT CTTCTCTCCT GGAGACCAGC CAGCCCCTTC TGGCCTTCCG
    GCTGGCCAGA CCAAGTCTCA GAGCCAGGAC CCGTTTGCAG ACCTTGGCGA CCTCAGTGCC
    GGCCTCCAAG GCTCGCCTGC CGCAGCTGCT CCTGGGGGCT TTGGTCCCAA AGCAGCGCCC
    CCTCCCAAGG CCAGCAGCTC CTGGCAGACG AGTCGGCCCC CAGCCCAGGG CACCCCGTGG
    CCCCCCCAGG CCAAGCCGCC CCCCAAAGCC AGTGTACCGC CAAGGCCTAA CTACACCTCC
    AGCTTCAGTG TGATCGGTAG CCGGGAGGAG CGGGGGGTCC GCGCCCCCAG CTTTGGTCCA
    AAGCCGAGGG TGTCAGAGAA CGACTTTGAA GACCTGCTCT CCAACCAGGG CTTCTCCTCG
    CGGGCTGACA GGAAGGGGCC GCGGACCATC GCTGAGATGA GGAGGCAGGA CCAGGCCCGG
    GACTTGGATC CGCTGAAGCT GAAGCTTCTG GAGTGGACGG AGGGCAAGGA GAGGAACATC
    CGCGCCCTGC TGTCCACGCT GCACACGGCG CTGTGGGACG GCGAGAGCCG CTGGACGCCG
    GTGGGCATGG CCGACCTGGT GACGCCCGCA CAAGTGAAGA AGCACTACCG CCGTGCCGTG
    CTGGTGGTCC ACCCCGACAA GGCCCGGGGG CAGCCCTACG AGCAGTATGC CCGGATGATC
    TTCATGGAGC TCAACGATGC GTGGGCTGAG TTTGAGAGCC AGGGCTCCCG GCCACTCTTC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa235257
    Clone ID Related Accession (Same CDS sequence) XM_010806358.3
    Accession Version XM_010806358.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3543bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product cyclin-G-associated kinase isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010806358.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAAAAAAC TTTCAGGCCA CCCAAACATT GTCCAGTTTT GTTCTGCAGC ATCGATAGGA 
    AAAGAGGAGT CGGACACTGG GCAGGCGGAG TTCCTGCTGC TCATGGAGCT CTGCAGAGGG
    CAGCTGGTGG AATTTTTAAA AAAAGTTGAA TCGAAAGGTC CGCTCTCGTG CGACACCGTC
    CTGAAGATCT TCTACCAGAC GTGCCGGGCG GTGCAGCACA TGCACAGACA GAAGCCGCCC
    ATCATCCACA GGGACCTCAA GGTTGAAAAC TTACTGCTTA GTAACCAGGG GACCATCAAG
    CTGTGTGACT TCGGCAGTGC CACGACCATC TCACACTACC CAGACTACAG CTGGAGCGCC
    CAGCGCCGGG CCCTGGTGGA GGAGGAGATC ACCAGGAACA CGACCCCCAT GTACAGGACG
    CCGGAAATTG TGGACTTGTA CTCAAACTTT CCGATTGGCG AGAAGCAGGA CATCTGGGCC
    CTGGGCTGCA TCCTGTACCT GCTCTGCTTC GGGCAGCACC CCTTTGAGGA CGGAGCGAAG
    CTTCGCATCG TCAACGGGAA GTACGCAATC CCTGCCGACG ACACGCGGTA CTCCGTGTTC
    CACAGCCTCA TCCGTTCCAT GCTCAAGGTC AACCCGGAGG AGCGGCTGTC CGTCACCGAG
    CTGGTCAACC AGCTCCAGGA GATCGCGGCC GCCCGCAACG TGAACCCCAA GTCACCCATC
    ACTGAGCTCC TGGAGCAGAA TGGAGGCTAT GGGCACGCGG CCCCTCCCGG CTCCACGGGC
    AGCAGTCTGG TGGTCACTGA GTACGAGCAG CCCTATGGCG GGTTCCTGGA CATTCTGCGT
    GGGGGCACCG AGCGCCTCTT CACCAACATC AAGGACACCT CCTCCAAAGT CATCCAGTCC
    GTTGCCAACT ATGCGAAGGG AGATCTGGAT ATTTCTTACA TCACGTCTAG AATCGCAGTC
    ATGTCGTTCC CGGCGGAGGG CGTGGAGTCG GCCATCAAGA ACAACATTGA GGATGTACGG
    CTGTTCCTGG ACTCGAAGCA CCCGGGCCGC TACGCCGTCT ACAACCTGTC CCCGCGGACC
    TACCGGCCCT CGAAGTTCCA CAACCGGGTC TCCGACTGCG GCTGGGTGGC CCGGCGGGCC
    CCGAGCCTCC GCAGCCTGTA CAGCCTCTGC AGGAACATGC ACGCCTGGCT GCGCCGCGAC
    CCTGGCAACG TCTGCGTGGT GCACTGCATG GACGGGAGGG CGGTGTCGGC CGTGGCCGTG
    TGCTCCTTCT TGTGCTTCTG CCGGCTCTTC AGCACCGCGG AGGCCGCTGT GTACATGTTC
    AGCATGAAGC GGTGCCCACC GGGCATCTGG CCATCCCACA AGAGGTACAT TGAGTACATG
    TGTGACATGG TGGCCGAGGA GCCCGTCACG CCCCACAGCA AGCCCATCCT GGTGAAGGCC
    GTGGTCATGA CGCCTGTGCC GCTCTTCAGC AAGCAGAGGA GCGGCTGCAG GCCCTTCTGC
    GAGGTCTACG TGGGGGATGA GCGCGTGACC ACCACGTCCC AGGAATACGA CAAGATGAGA
    GACTTTAAAA TTGAGGATGG CAAAGCGGTC ATTCCCCTGG GCATAACAGT CCAGGGGGAC
    GTGCTCATCA TCATCTATCA TGCGAGGTCC ACCCTGGGAG GAAGGCTGCA GGCCAAGATG
    GCGTCCATGA AGATGTTTCA GATCCAGTTT CACACAGGGT TCGTGCCTCG GAACGCAGCT
    ACTGTGAAGT TTGCCAAGTA TGATCTGGAT GCTTGCGACA TCCAGGAGAA GTACCCCGAC
    TTGTTCCAAG TGAGCCTGGA GGTGGAGGTG GAGCCCAGAG ACAGGCCCGG CCACGAGGCC
    CCGCCCTGGG AGGGTGCAGG CATGCGAGGG CTGAACCCCA AGATCCTCTT CTCCAGCCGG
    GAGGAGCAGC AAGATGTCCT GTCCAAGTTT GGGAAGCCAG AGCTCCCCAG GCAGCCTGGG
    TCCACCGCAC AGTACGACGC CGAGGCCGGG CCCGCGGATG CCGAGCCCCC CGAGTCAGAC
    TCGCCGCACA GCAGCGTGGA TGCTGACCAC TTCCTGCACA CGCTGGACTG GCGGGAGGAT
    CGGGACTCCA GTGTGGGCCC GGAGGGTCAC CTGGCCGAGG AGGGTCCCTC TGCTGTGGCC
    GAGGACGGCT GTGTGAGTGA GCCATCGGAC GAGGAAGCCC CGACACCCTC TGCGGAGAGC
    GGGGACACAG GCGATGAGGA CGTGCCCGCC CCCGCCGCCC CGCAGGACGA CAGCGTGGAC
    CTTCTGGGGC TGCACGCGGA GGCGGGGCCT GTGCCCGCCC CCCAGGCCCC CGGCGGCCCC
    CCCAGCAATG CAGACCTGCT CGGCTGCCTG CTCGGGGCCC CGGGCCCCGC TCCGGAGGGT
    GCTGTGGGGG GCCTGCTCGA CGGGGATGCG GCCCTGGTCT TCAGCAGCCC AGCCCCCGCG
    CCTTGCACCC AGACAACTGC CCCGGCCGCT GACCCGTTCG GCCCGCTCCT GCTGCCGCCC
    GACGTGGACA CCCCTCCTAG CTCGCAGCCT GACCTCTTCG GGGAATTCCT CAACTCAGAC
    GCCCCGGCCG CCCAGCCCGC GCCCTTCCCC TCCACCCACA GCGCTCCGCC GCCAGCCTGC
    AGCACCGACT TCCTGCATCT TGGGGACCTG CCCGCCGAGC CCAGCAAGAT GACAGCCTCT
    GCCAGCCACC CCGACCTGCT GGGAGCCTGG GATGCCTGGG CTGAGGCGCC GGCCCCCGCG
    CTGGCTGCAG ACGGCACCTT CTTCTCTCCT GGAGACCAGC CAGCCCCTTC TGGCCTTCCG
    GCTGGCCAGA CCAAGTCTCA GAGCCAGGAC CCGTTTGCAG ACCTTGGCGA CCTCAGTGCC
    GGCCTCCAAG GCTCGCCTGC CGCAGCTGCT CCTGGGGGCT TTGGTCCCAA AGCAGCGCCC
    CCTCCCAAGG CCAGCAGCTC CTGGCAGACG AGTCGGCCCC CAGCCCAGGG CACCCCGTGG
    CCCCCCCAGG CCAAGCCGCC CCCCAAAGCC AGTGTACCGC CAAGGCCTAA CTACACCTCC
    AGCTTCAGTG TGATCGGTAG CCGGGAGGAG CGGGGGGTCC GCGCCCCCAG CTTTGGTCCA
    AAGCCGAGGG TGTCAGAGAA CGACTTTGAA GACCTGCTCT CCAACCAGGG CTTCTCCTCG
    CGGGCTGACA GGAAGGGGCC GCGGACCATC GCTGAGATGA GGAGGCAGGA CCAGGCCCGG
    GACTTGGATC CGCTGAAGCT GAAGCTTCTG GAGTGGACGG AGGGCAAGGA GAGGAACATC
    CGCGCCCTGC TGTCCACGCT GCACACGGCG CTGTGGGACG GCGAGAGCCG CTGGACGCCG
    GTGGGCATGG CCGACCTGGT GACGCCCGCA CAAGTGAAGA AGCACTACCG CCGTGCCGTG
    CTGGTGGTCC ACCCCGACAA GGCCCGGGGG CAGCCCTACG AGCAGTATGC CCGGATGATC
    TTCATGGAGC TCAACGATGC GTGGGCTGAG TTTGAGAGCC AGGGCTCCCG GCCACTCTTC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010804660.2
    CDS230..3772
    Translation

    Target ORF information:

    RefSeq Version XM_010806358.3
    Organism Bos taurus(cattle)
    Definition Bos taurus cyclin G associated kinase (GAK), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806358.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAAAAAAC TTTCAGGCCA CCCAAACATT GTCCAGTTTT GTTCTGCAGC ATCGATAGGA 
    AAAGAGGAGT CGGACACTGG GCAGGCGGAG TTCCTGCTGC TCATGGAGCT CTGCAGAGGG
    CAGCTGGTGG AATTTTTAAA AAAAGTTGAA TCGAAAGGTC CGCTCTCGTG CGACACCGTC
    CTGAAGATCT TCTACCAGAC GTGCCGGGCG GTGCAGCACA TGCACAGACA GAAGCCGCCC
    ATCATCCACA GGGACCTCAA GGTTGAAAAC TTACTGCTTA GTAACCAGGG GACCATCAAG
    CTGTGTGACT TCGGCAGTGC CACGACCATC TCACACTACC CAGACTACAG CTGGAGCGCC
    CAGCGCCGGG CCCTGGTGGA GGAGGAGATC ACCAGGAACA CGACCCCCAT GTACAGGACG
    CCGGAAATTG TGGACTTGTA CTCAAACTTT CCGATTGGCG AGAAGCAGGA CATCTGGGCC
    CTGGGCTGCA TCCTGTACCT GCTCTGCTTC GGGCAGCACC CCTTTGAGGA CGGAGCGAAG
    CTTCGCATCG TCAACGGGAA GTACGCAATC CCTGCCGACG ACACGCGGTA CTCCGTGTTC
    CACAGCCTCA TCCGTTCCAT GCTCAAGGTC AACCCGGAGG AGCGGCTGTC CGTCACCGAG
    CTGGTCAACC AGCTCCAGGA GATCGCGGCC GCCCGCAACG TGAACCCCAA GTCACCCATC
    ACTGAGCTCC TGGAGCAGAA TGGAGGCTAT GGGCACGCGG CCCCTCCCGG CTCCACGGGC
    AGCAGTCTGG TGGTCACTGA GTACGAGCAG CCCTATGGCG GGTTCCTGGA CATTCTGCGT
    GGGGGCACCG AGCGCCTCTT CACCAACATC AAGGACACCT CCTCCAAAGT CATCCAGTCC
    GTTGCCAACT ATGCGAAGGG AGATCTGGAT ATTTCTTACA TCACGTCTAG AATCGCAGTC
    ATGTCGTTCC CGGCGGAGGG CGTGGAGTCG GCCATCAAGA ACAACATTGA GGATGTACGG
    CTGTTCCTGG ACTCGAAGCA CCCGGGCCGC TACGCCGTCT ACAACCTGTC CCCGCGGACC
    TACCGGCCCT CGAAGTTCCA CAACCGGGTC TCCGACTGCG GCTGGGTGGC CCGGCGGGCC
    CCGAGCCTCC GCAGCCTGTA CAGCCTCTGC AGGAACATGC ACGCCTGGCT GCGCCGCGAC
    CCTGGCAACG TCTGCGTGGT GCACTGCATG GACGGGAGGG CGGTGTCGGC CGTGGCCGTG
    TGCTCCTTCT TGTGCTTCTG CCGGCTCTTC AGCACCGCGG AGGCCGCTGT GTACATGTTC
    AGCATGAAGC GGTGCCCACC GGGCATCTGG CCATCCCACA AGAGGTACAT TGAGTACATG
    TGTGACATGG TGGCCGAGGA GCCCGTCACG CCCCACAGCA AGCCCATCCT GGTGAAGGCC
    GTGGTCATGA CGCCTGTGCC GCTCTTCAGC AAGCAGAGGA GCGGCTGCAG GCCCTTCTGC
    GAGGTCTACG TGGGGGATGA GCGCGTGACC ACCACGTCCC AGGAATACGA CAAGATGAGA
    GACTTTAAAA TTGAGGATGG CAAAGCGGTC ATTCCCCTGG GCATAACAGT CCAGGGGGAC
    GTGCTCATCA TCATCTATCA TGCGAGGTCC ACCCTGGGAG GAAGGCTGCA GGCCAAGATG
    GCGTCCATGA AGATGTTTCA GATCCAGTTT CACACAGGGT TCGTGCCTCG GAACGCAGCT
    ACTGTGAAGT TTGCCAAGTA TGATCTGGAT GCTTGCGACA TCCAGGAGAA GTACCCCGAC
    TTGTTCCAAG TGAGCCTGGA GGTGGAGGTG GAGCCCAGAG ACAGGCCCGG CCACGAGGCC
    CCGCCCTGGG AGGGTGCAGG CATGCGAGGG CTGAACCCCA AGATCCTCTT CTCCAGCCGG
    GAGGAGCAGC AAGATGTCCT GTCCAAGTTT GGGAAGCCAG AGCTCCCCAG GCAGCCTGGG
    TCCACCGCAC AGTACGACGC CGAGGCCGGG CCCGCGGATG CCGAGCCCCC CGAGTCAGAC
    TCGCCGCACA GCAGCGTGGA TGCTGACCAC TTCCTGCACA CGCTGGACTG GCGGGAGGAT
    CGGGACTCCA GTGTGGGCCC GGAGGGTCAC CTGGCCGAGG AGGGTCCCTC TGCTGTGGCC
    GAGGACGGCT GTGTGAGTGA GCCATCGGAC GAGGAAGCCC CGACACCCTC TGCGGAGAGC
    GGGGACACAG GCGATGAGGA CGTGCCCGCC CCCGCCGCCC CGCAGGACGA CAGCGTGGAC
    CTTCTGGGGC TGCACGCGGA GGCGGGGCCT GTGCCCGCCC CCCAGGCCCC CGGCGGCCCC
    CCCAGCAATG CAGACCTGCT CGGCTGCCTG CTCGGGGCCC CGGGCCCCGC TCCGGAGGGT
    GCTGTGGGGG GCCTGCTCGA CGGGGATGCG GCCCTGGTCT TCAGCAGCCC AGCCCCCGCG
    CCTTGCACCC AGACAACTGC CCCGGCCGCT GACCCGTTCG GCCCGCTCCT GCTGCCGCCC
    GACGTGGACA CCCCTCCTAG CTCGCAGCCT GACCTCTTCG GGGAATTCCT CAACTCAGAC
    GCCCCGGCCG CCCAGCCCGC GCCCTTCCCC TCCACCCACA GCGCTCCGCC GCCAGCCTGC
    AGCACCGACT TCCTGCATCT TGGGGACCTG CCCGCCGAGC CCAGCAAGAT GACAGCCTCT
    GCCAGCCACC CCGACCTGCT GGGAGCCTGG GATGCCTGGG CTGAGGCGCC GGCCCCCGCG
    CTGGCTGCAG ACGGCACCTT CTTCTCTCCT GGAGACCAGC CAGCCCCTTC TGGCCTTCCG
    GCTGGCCAGA CCAAGTCTCA GAGCCAGGAC CCGTTTGCAG ACCTTGGCGA CCTCAGTGCC
    GGCCTCCAAG GCTCGCCTGC CGCAGCTGCT CCTGGGGGCT TTGGTCCCAA AGCAGCGCCC
    CCTCCCAAGG CCAGCAGCTC CTGGCAGACG AGTCGGCCCC CAGCCCAGGG CACCCCGTGG
    CCCCCCCAGG CCAAGCCGCC CCCCAAAGCC AGTGTACCGC CAAGGCCTAA CTACACCTCC
    AGCTTCAGTG TGATCGGTAG CCGGGAGGAG CGGGGGGTCC GCGCCCCCAG CTTTGGTCCA
    AAGCCGAGGG TGTCAGAGAA CGACTTTGAA GACCTGCTCT CCAACCAGGG CTTCTCCTCG
    CGGGCTGACA GGAAGGGGCC GCGGACCATC GCTGAGATGA GGAGGCAGGA CCAGGCCCGG
    GACTTGGATC CGCTGAAGCT GAAGCTTCTG GAGTGGACGG AGGGCAAGGA GAGGAACATC
    CGCGCCCTGC TGTCCACGCT GCACACGGCG CTGTGGGACG GCGAGAGCCG CTGGACGCCG
    GTGGGCATGG CCGACCTGGT GACGCCCGCA CAAGTGAAGA AGCACTACCG CCGTGCCGTG
    CTGGTGGTCC ACCCCGACAA GGCCCGGGGG CAGCCCTACG AGCAGTATGC CCGGATGATC
    TTCATGGAGC TCAACGATGC GTGGGCTGAG TTTGAGAGCC AGGGCTCCCG GCCACTCTTC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa235258
    Clone ID Related Accession (Same CDS sequence) XM_005208283.2 , XM_024993152.1 , XM_024993153.1
    Accession Version XM_005208283.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3441bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product cyclin-G-associated kinase isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005208283.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGAGCTCT GCAGAGGGCA GCTGGTGGAA TTTTTAAAAA AAGTTGAATC GAAAGGTCCG 
    CTCTCGTGCG ACACCGTCCT GAAGATCTTC TACCAGACGT GCCGGGCGGT GCAGCACATG
    CACAGACAGA AGCCGCCCAT CATCCACAGG GACCTCAAGG TTGAAAACTT ACTGCTTAGT
    AACCAGGGGA CCATCAAGCT GTGTGACTTC GGCAGTGCCA CGACCATCTC ACACTACCCA
    GACTACAGCT GGAGCGCCCA GCGCCGGGCC CTGGTGGAGG AGGAGATCAC CAGGAACACG
    ACCCCCATGT ACAGGACGCC GGAAATTGTG GACTTGTACT CAAACTTTCC GATTGGCGAG
    AAGCAGGACA TCTGGGCCCT GGGCTGCATC CTGTACCTGC TCTGCTTCGG GCAGCACCCC
    TTTGAGGACG GAGCGAAGCT TCGCATCGTC AACGGGAAGT ACGCAATCCC TGCCGACGAC
    ACGCGGTACT CCGTGTTCCA CAGCCTCATC CGTTCCATGC TCAAGGTCAA CCCGGAGGAG
    CGGCTGTCCG TCACCGAGCT GGTCAACCAG CTCCAGGAGA TCGCGGCCGC CCGCAACGTG
    AACCCCAAGT CACCCATCAC TGAGCTCCTG GAGCAGAATG GAGGCTATGG GCACGCGGCC
    CCTCCCGGCT CCACGGGCAG CAGTCTGGTG GTCACTGAGT ACGAGCAGCC CTATGGCGGG
    TTCCTGGACA TTCTGCGTGG GGGCACCGAG CGCCTCTTCA CCAACATCAA GGACACCTCC
    TCCAAAGTCA TCCAGTCCGT TGCCAACTAT GCGAAGGGAG ATCTGGATAT TTCTTACATC
    ACGTCTAGAA TCGCAGTCAT GTCGTTCCCG GCGGAGGGCG TGGAGTCGGC CATCAAGAAC
    AACATTGAGG ATGTACGGCT GTTCCTGGAC TCGAAGCACC CGGGCCGCTA CGCCGTCTAC
    AACCTGTCCC CGCGGACCTA CCGGCCCTCG AAGTTCCACA ACCGGGTCTC CGACTGCGGC
    TGGGTGGCCC GGCGGGCCCC GAGCCTCCGC AGCCTGTACA GCCTCTGCAG GAACATGCAC
    GCCTGGCTGC GCCGCGACCC TGGCAACGTC TGCGTGGTGC ACTGCATGGA CGGGAGGGCG
    GTGTCGGCCG TGGCCGTGTG CTCCTTCTTG TGCTTCTGCC GGCTCTTCAG CACCGCGGAG
    GCCGCTGTGT ACATGTTCAG CATGAAGCGG TGCCCACCGG GCATCTGGCC ATCCCACAAG
    AGGTACATTG AGTACATGTG TGACATGGTG GCCGAGGAGC CCGTCACGCC CCACAGCAAG
    CCCATCCTGG TGAAGGCCGT GGTCATGACG CCTGTGCCGC TCTTCAGCAA GCAGAGGAGC
    GGCTGCAGGC CCTTCTGCGA GGTCTACGTG GGGGATGAGC GCGTGACCAC CACGTCCCAG
    GAATACGACA AGATGAGAGA CTTTAAAATT GAGGATGGCA AAGCGGTCAT TCCCCTGGGC
    ATAACAGTCC AGGGGGACGT GCTCATCATC ATCTATCATG CGAGGTCCAC CCTGGGAGGA
    AGGCTGCAGG CCAAGATGGC GTCCATGAAG ATGTTTCAGA TCCAGTTTCA CACAGGGTTC
    GTGCCTCGGA ACGCAGCTAC TGTGAAGTTT GCCAAGTATG ATCTGGATGC TTGCGACATC
    CAGGAGAAGT ACCCCGACTT GTTCCAAGTG AGCCTGGAGG TGGAGGTGGA GCCCAGAGAC
    AGGCCCGGCC ACGAGGCCCC GCCCTGGGAG GGTGCAGGCA TGCGAGGGCT GAACCCCAAG
    ATCCTCTTCT CCAGCCGGGA GGAGCAGCAA GATGTCCTGT CCAAGTTTGG GAAGCCAGAG
    CTCCCCAGGC AGCCTGGGTC CACCGCACAG TACGACGCCG AGGCCGGGCC CGCGGATGCC
    GAGCCCCCCG AGTCAGACTC GCCGCACAGC AGCGTGGATG CTGACCACTT CCTGCACACG
    CTGGACTGGC GGGAGGATCG GGACTCCAGT GTGGGCCCGG AGGGTCACCT GGCCGAGGAG
    GGTCCCTCTG CTGTGGCCGA GGACGGCTGT GTGAGTGAGC CATCGGACGA GGAAGCCCCG
    ACACCCTCTG CGGAGAGCGG GGACACAGGC GATGAGGACG TGCCCGCCCC CGCCGCCCCG
    CAGGACGACA GCGTGGACCT TCTGGGGCTG CACGCGGAGG CGGGGCCTGT GCCCGCCCCC
    CAGGCCCCCG GCGGCCCCCC CAGCAATGCA GACCTGCTCG GCTGCCTGCT CGGGGCCCCG
    GGCCCCGCTC CGGAGGGTGC TGTGGGGGGC CTGCTCGACG GGGATGCGGC CCTGGTCTTC
    AGCAGCCCAG CCCCCGCGCC TTGCACCCAG ACAACTGCCC CGGCCGCTGA CCCGTTCGGC
    CCGCTCCTGC TGCCGCCCGA CGTGGACACC CCTCCTAGCT CGCAGCCTGA CCTCTTCGGG
    GAATTCCTCA ACTCAGACGC CCCGGCCGCC CAGCCCGCGC CCTTCCCCTC CACCCACAGC
    GCTCCGCCGC CAGCCTGCAG CACCGACTTC CTGCATCTTG GGGACCTGCC CGCCGAGCCC
    AGCAAGATGA CAGCCTCTGC CAGCCACCCC GACCTGCTGG GAGCCTGGGA TGCCTGGGCT
    GAGGCGCCGG CCCCCGCGCT GGCTGCAGAC GGCACCTTCT TCTCTCCTGG AGACCAGCCA
    GCCCCTTCTG GCCTTCCGGC TGGCCAGACC AAGTCTCAGA GCCAGGACCC GTTTGCAGAC
    CTTGGCGACC TCAGTGCCGG CCTCCAAGGC TCGCCTGCCG CAGCTGCTCC TGGGGGCTTT
    GGTCCCAAAG CAGCGCCCCC TCCCAAGGCC AGCAGCTCCT GGCAGACGAG TCGGCCCCCA
    GCCCAGGGCA CCCCGTGGCC CCCCCAGGCC AAGCCGCCCC CCAAAGCCAG TGTACCGCCA
    AGGCCTAACT ACACCTCCAG CTTCAGTGTG ATCGGTAGCC GGGAGGAGCG GGGGGTCCGC
    GCCCCCAGCT TTGGTCCAAA GCCGAGGGTG TCAGAGAACG ACTTTGAAGA CCTGCTCTCC
    AACCAGGGCT TCTCCTCGCG GGCTGACAGG AAGGGGCCGC GGACCATCGC TGAGATGAGG
    AGGCAGGACC AGGCCCGGGA CTTGGATCCG CTGAAGCTGA AGCTTCTGGA GTGGACGGAG
    GGCAAGGAGA GGAACATCCG CGCCCTGCTG TCCACGCTGC ACACGGCGCT GTGGGACGGC
    GAGAGCCGCT GGACGCCGGT GGGCATGGCC GACCTGGTGA CGCCCGCACA AGTGAAGAAG
    CACTACCGCC GTGCCGTGCT GGTGGTCCAC CCCGACAAGG CCCGGGGGCA GCCCTACGAG
    CAGTATGCCC GGATGATCTT CATGGAGCTC AACGATGCGT GGGCTGAGTT TGAGAGCCAG
    GGCTCCCGGC CACTCTTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005208340.2
    CDS726..4166
    Translation

    Target ORF information:

    RefSeq Version XM_005208283.2
    Organism Bos taurus(cattle)
    Definition Bos taurus cyclin G associated kinase (GAK), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005208283.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGAGCTCT GCAGAGGGCA GCTGGTGGAA TTTTTAAAAA AAGTTGAATC GAAAGGTCCG 
    CTCTCGTGCG ACACCGTCCT GAAGATCTTC TACCAGACGT GCCGGGCGGT GCAGCACATG
    CACAGACAGA AGCCGCCCAT CATCCACAGG GACCTCAAGG TTGAAAACTT ACTGCTTAGT
    AACCAGGGGA CCATCAAGCT GTGTGACTTC GGCAGTGCCA CGACCATCTC ACACTACCCA
    GACTACAGCT GGAGCGCCCA GCGCCGGGCC CTGGTGGAGG AGGAGATCAC CAGGAACACG
    ACCCCCATGT ACAGGACGCC GGAAATTGTG GACTTGTACT CAAACTTTCC GATTGGCGAG
    AAGCAGGACA TCTGGGCCCT GGGCTGCATC CTGTACCTGC TCTGCTTCGG GCAGCACCCC
    TTTGAGGACG GAGCGAAGCT TCGCATCGTC AACGGGAAGT ACGCAATCCC TGCCGACGAC
    ACGCGGTACT CCGTGTTCCA CAGCCTCATC CGTTCCATGC TCAAGGTCAA CCCGGAGGAG
    CGGCTGTCCG TCACCGAGCT GGTCAACCAG CTCCAGGAGA TCGCGGCCGC CCGCAACGTG
    AACCCCAAGT CACCCATCAC TGAGCTCCTG GAGCAGAATG GAGGCTATGG GCACGCGGCC
    CCTCCCGGCT CCACGGGCAG CAGTCTGGTG GTCACTGAGT ACGAGCAGCC CTATGGCGGG
    TTCCTGGACA TTCTGCGTGG GGGCACCGAG CGCCTCTTCA CCAACATCAA GGACACCTCC
    TCCAAAGTCA TCCAGTCCGT TGCCAACTAT GCGAAGGGAG ATCTGGATAT TTCTTACATC
    ACGTCTAGAA TCGCAGTCAT GTCGTTCCCG GCGGAGGGCG TGGAGTCGGC CATCAAGAAC
    AACATTGAGG ATGTACGGCT GTTCCTGGAC TCGAAGCACC CGGGCCGCTA CGCCGTCTAC
    AACCTGTCCC CGCGGACCTA CCGGCCCTCG AAGTTCCACA ACCGGGTCTC CGACTGCGGC
    TGGGTGGCCC GGCGGGCCCC GAGCCTCCGC AGCCTGTACA GCCTCTGCAG GAACATGCAC
    GCCTGGCTGC GCCGCGACCC TGGCAACGTC TGCGTGGTGC ACTGCATGGA CGGGAGGGCG
    GTGTCGGCCG TGGCCGTGTG CTCCTTCTTG TGCTTCTGCC GGCTCTTCAG CACCGCGGAG
    GCCGCTGTGT ACATGTTCAG CATGAAGCGG TGCCCACCGG GCATCTGGCC ATCCCACAAG
    AGGTACATTG AGTACATGTG TGACATGGTG GCCGAGGAGC CCGTCACGCC CCACAGCAAG
    CCCATCCTGG TGAAGGCCGT GGTCATGACG CCTGTGCCGC TCTTCAGCAA GCAGAGGAGC
    GGCTGCAGGC CCTTCTGCGA GGTCTACGTG GGGGATGAGC GCGTGACCAC CACGTCCCAG
    GAATACGACA AGATGAGAGA CTTTAAAATT GAGGATGGCA AAGCGGTCAT TCCCCTGGGC
    ATAACAGTCC AGGGGGACGT GCTCATCATC ATCTATCATG CGAGGTCCAC CCTGGGAGGA
    AGGCTGCAGG CCAAGATGGC GTCCATGAAG ATGTTTCAGA TCCAGTTTCA CACAGGGTTC
    GTGCCTCGGA ACGCAGCTAC TGTGAAGTTT GCCAAGTATG ATCTGGATGC TTGCGACATC
    CAGGAGAAGT ACCCCGACTT GTTCCAAGTG AGCCTGGAGG TGGAGGTGGA GCCCAGAGAC
    AGGCCCGGCC ACGAGGCCCC GCCCTGGGAG GGTGCAGGCA TGCGAGGGCT GAACCCCAAG
    ATCCTCTTCT CCAGCCGGGA GGAGCAGCAA GATGTCCTGT CCAAGTTTGG GAAGCCAGAG
    CTCCCCAGGC AGCCTGGGTC CACCGCACAG TACGACGCCG AGGCCGGGCC CGCGGATGCC
    GAGCCCCCCG AGTCAGACTC GCCGCACAGC AGCGTGGATG CTGACCACTT CCTGCACACG
    CTGGACTGGC GGGAGGATCG GGACTCCAGT GTGGGCCCGG AGGGTCACCT GGCCGAGGAG
    GGTCCCTCTG CTGTGGCCGA GGACGGCTGT GTGAGTGAGC CATCGGACGA GGAAGCCCCG
    ACACCCTCTG CGGAGAGCGG GGACACAGGC GATGAGGACG TGCCCGCCCC CGCCGCCCCG
    CAGGACGACA GCGTGGACCT TCTGGGGCTG CACGCGGAGG CGGGGCCTGT GCCCGCCCCC
    CAGGCCCCCG GCGGCCCCCC CAGCAATGCA GACCTGCTCG GCTGCCTGCT CGGGGCCCCG
    GGCCCCGCTC CGGAGGGTGC TGTGGGGGGC CTGCTCGACG GGGATGCGGC CCTGGTCTTC
    AGCAGCCCAG CCCCCGCGCC TTGCACCCAG ACAACTGCCC CGGCCGCTGA CCCGTTCGGC
    CCGCTCCTGC TGCCGCCCGA CGTGGACACC CCTCCTAGCT CGCAGCCTGA CCTCTTCGGG
    GAATTCCTCA ACTCAGACGC CCCGGCCGCC CAGCCCGCGC CCTTCCCCTC CACCCACAGC
    GCTCCGCCGC CAGCCTGCAG CACCGACTTC CTGCATCTTG GGGACCTGCC CGCCGAGCCC
    AGCAAGATGA CAGCCTCTGC CAGCCACCCC GACCTGCTGG GAGCCTGGGA TGCCTGGGCT
    GAGGCGCCGG CCCCCGCGCT GGCTGCAGAC GGCACCTTCT TCTCTCCTGG AGACCAGCCA
    GCCCCTTCTG GCCTTCCGGC TGGCCAGACC AAGTCTCAGA GCCAGGACCC GTTTGCAGAC
    CTTGGCGACC TCAGTGCCGG CCTCCAAGGC TCGCCTGCCG CAGCTGCTCC TGGGGGCTTT
    GGTCCCAAAG CAGCGCCCCC TCCCAAGGCC AGCAGCTCCT GGCAGACGAG TCGGCCCCCA
    GCCCAGGGCA CCCCGTGGCC CCCCCAGGCC AAGCCGCCCC CCAAAGCCAG TGTACCGCCA
    AGGCCTAACT ACACCTCCAG CTTCAGTGTG ATCGGTAGCC GGGAGGAGCG GGGGGTCCGC
    GCCCCCAGCT TTGGTCCAAA GCCGAGGGTG TCAGAGAACG ACTTTGAAGA CCTGCTCTCC
    AACCAGGGCT TCTCCTCGCG GGCTGACAGG AAGGGGCCGC GGACCATCGC TGAGATGAGG
    AGGCAGGACC AGGCCCGGGA CTTGGATCCG CTGAAGCTGA AGCTTCTGGA GTGGACGGAG
    GGCAAGGAGA GGAACATCCG CGCCCTGCTG TCCACGCTGC ACACGGCGCT GTGGGACGGC
    GAGAGCCGCT GGACGCCGGT GGGCATGGCC GACCTGGTGA CGCCCGCACA AGTGAAGAAG
    CACTACCGCC GTGCCGTGCT GGTGGTCCAC CCCGACAAGG CCCGGGGGCA GCCCTACGAG
    CAGTATGCCC GGATGATCTT CATGGAGCTC AACGATGCGT GGGCTGAGTT TGAGAGCCAG
    GGCTCCCGGC CACTCTTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa235258
    Clone ID Related Accession (Same CDS sequence) XM_005208283.2 , XM_024993152.1 , XM_024993153.1
    Accession Version XM_024993152.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3441bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product cyclin-G-associated kinase isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGAGCTCT GCAGAGGGCA GCTGGTGGAA TTTTTAAAAA AAGTTGAATC GAAAGGTCCG 
    CTCTCGTGCG ACACCGTCCT GAAGATCTTC TACCAGACGT GCCGGGCGGT GCAGCACATG
    CACAGACAGA AGCCGCCCAT CATCCACAGG GACCTCAAGG TTGAAAACTT ACTGCTTAGT
    AACCAGGGGA CCATCAAGCT GTGTGACTTC GGCAGTGCCA CGACCATCTC ACACTACCCA
    GACTACAGCT GGAGCGCCCA GCGCCGGGCC CTGGTGGAGG AGGAGATCAC CAGGAACACG
    ACCCCCATGT ACAGGACGCC GGAAATTGTG GACTTGTACT CAAACTTTCC GATTGGCGAG
    AAGCAGGACA TCTGGGCCCT GGGCTGCATC CTGTACCTGC TCTGCTTCGG GCAGCACCCC
    TTTGAGGACG GAGCGAAGCT TCGCATCGTC AACGGGAAGT ACGCAATCCC TGCCGACGAC
    ACGCGGTACT CCGTGTTCCA CAGCCTCATC CGTTCCATGC TCAAGGTCAA CCCGGAGGAG
    CGGCTGTCCG TCACCGAGCT GGTCAACCAG CTCCAGGAGA TCGCGGCCGC CCGCAACGTG
    AACCCCAAGT CACCCATCAC TGAGCTCCTG GAGCAGAATG GAGGCTATGG GCACGCGGCC
    CCTCCCGGCT CCACGGGCAG CAGTCTGGTG GTCACTGAGT ACGAGCAGCC CTATGGCGGG
    TTCCTGGACA TTCTGCGTGG GGGCACCGAG CGCCTCTTCA CCAACATCAA GGACACCTCC
    TCCAAAGTCA TCCAGTCCGT TGCCAACTAT GCGAAGGGAG ATCTGGATAT TTCTTACATC
    ACGTCTAGAA TCGCAGTCAT GTCGTTCCCG GCGGAGGGCG TGGAGTCGGC CATCAAGAAC
    AACATTGAGG ATGTACGGCT GTTCCTGGAC TCGAAGCACC CGGGCCGCTA CGCCGTCTAC
    AACCTGTCCC CGCGGACCTA CCGGCCCTCG AAGTTCCACA ACCGGGTCTC CGACTGCGGC
    TGGGTGGCCC GGCGGGCCCC GAGCCTCCGC AGCCTGTACA GCCTCTGCAG GAACATGCAC
    GCCTGGCTGC GCCGCGACCC TGGCAACGTC TGCGTGGTGC ACTGCATGGA CGGGAGGGCG
    GTGTCGGCCG TGGCCGTGTG CTCCTTCTTG TGCTTCTGCC GGCTCTTCAG CACCGCGGAG
    GCCGCTGTGT ACATGTTCAG CATGAAGCGG TGCCCACCGG GCATCTGGCC ATCCCACAAG
    AGGTACATTG AGTACATGTG TGACATGGTG GCCGAGGAGC CCGTCACGCC CCACAGCAAG
    CCCATCCTGG TGAAGGCCGT GGTCATGACG CCTGTGCCGC TCTTCAGCAA GCAGAGGAGC
    GGCTGCAGGC CCTTCTGCGA GGTCTACGTG GGGGATGAGC GCGTGACCAC CACGTCCCAG
    GAATACGACA AGATGAGAGA CTTTAAAATT GAGGATGGCA AAGCGGTCAT TCCCCTGGGC
    ATAACAGTCC AGGGGGACGT GCTCATCATC ATCTATCATG CGAGGTCCAC CCTGGGAGGA
    AGGCTGCAGG CCAAGATGGC GTCCATGAAG ATGTTTCAGA TCCAGTTTCA CACAGGGTTC
    GTGCCTCGGA ACGCAGCTAC TGTGAAGTTT GCCAAGTATG ATCTGGATGC TTGCGACATC
    CAGGAGAAGT ACCCCGACTT GTTCCAAGTG AGCCTGGAGG TGGAGGTGGA GCCCAGAGAC
    AGGCCCGGCC ACGAGGCCCC GCCCTGGGAG GGTGCAGGCA TGCGAGGGCT GAACCCCAAG
    ATCCTCTTCT CCAGCCGGGA GGAGCAGCAA GATGTCCTGT CCAAGTTTGG GAAGCCAGAG
    CTCCCCAGGC AGCCTGGGTC CACCGCACAG TACGACGCCG AGGCCGGGCC CGCGGATGCC
    GAGCCCCCCG AGTCAGACTC GCCGCACAGC AGCGTGGATG CTGACCACTT CCTGCACACG
    CTGGACTGGC GGGAGGATCG GGACTCCAGT GTGGGCCCGG AGGGTCACCT GGCCGAGGAG
    GGTCCCTCTG CTGTGGCCGA GGACGGCTGT GTGAGTGAGC CATCGGACGA GGAAGCCCCG
    ACACCCTCTG CGGAGAGCGG GGACACAGGC GATGAGGACG TGCCCGCCCC CGCCGCCCCG
    CAGGACGACA GCGTGGACCT TCTGGGGCTG CACGCGGAGG CGGGGCCTGT GCCCGCCCCC
    CAGGCCCCCG GCGGCCCCCC CAGCAATGCA GACCTGCTCG GCTGCCTGCT CGGGGCCCCG
    GGCCCCGCTC CGGAGGGTGC TGTGGGGGGC CTGCTCGACG GGGATGCGGC CCTGGTCTTC
    AGCAGCCCAG CCCCCGCGCC TTGCACCCAG ACAACTGCCC CGGCCGCTGA CCCGTTCGGC
    CCGCTCCTGC TGCCGCCCGA CGTGGACACC CCTCCTAGCT CGCAGCCTGA CCTCTTCGGG
    GAATTCCTCA ACTCAGACGC CCCGGCCGCC CAGCCCGCGC CCTTCCCCTC CACCCACAGC
    GCTCCGCCGC CAGCCTGCAG CACCGACTTC CTGCATCTTG GGGACCTGCC CGCCGAGCCC
    AGCAAGATGA CAGCCTCTGC CAGCCACCCC GACCTGCTGG GAGCCTGGGA TGCCTGGGCT
    GAGGCGCCGG CCCCCGCGCT GGCTGCAGAC GGCACCTTCT TCTCTCCTGG AGACCAGCCA
    GCCCCTTCTG GCCTTCCGGC TGGCCAGACC AAGTCTCAGA GCCAGGACCC GTTTGCAGAC
    CTTGGCGACC TCAGTGCCGG CCTCCAAGGC TCGCCTGCCG CAGCTGCTCC TGGGGGCTTT
    GGTCCCAAAG CAGCGCCCCC TCCCAAGGCC AGCAGCTCCT GGCAGACGAG TCGGCCCCCA
    GCCCAGGGCA CCCCGTGGCC CCCCCAGGCC AAGCCGCCCC CCAAAGCCAG TGTACCGCCA
    AGGCCTAACT ACACCTCCAG CTTCAGTGTG ATCGGTAGCC GGGAGGAGCG GGGGGTCCGC
    GCCCCCAGCT TTGGTCCAAA GCCGAGGGTG TCAGAGAACG ACTTTGAAGA CCTGCTCTCC
    AACCAGGGCT TCTCCTCGCG GGCTGACAGG AAGGGGCCGC GGACCATCGC TGAGATGAGG
    AGGCAGGACC AGGCCCGGGA CTTGGATCCG CTGAAGCTGA AGCTTCTGGA GTGGACGGAG
    GGCAAGGAGA GGAACATCCG CGCCCTGCTG TCCACGCTGC ACACGGCGCT GTGGGACGGC
    GAGAGCCGCT GGACGCCGGT GGGCATGGCC GACCTGGTGA CGCCCGCACA AGTGAAGAAG
    CACTACCGCC GTGCCGTGCT GGTGGTCCAC CCCGACAAGG CCCGGGGGCA GCCCTACGAG
    CAGTATGCCC GGATGATCTT CATGGAGCTC AACGATGCGT GGGCTGAGTT TGAGAGCCAG
    GGCTCCCGGC CACTCTTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024848920.1
    CDS783..4223
    Translation

    Target ORF information:

    RefSeq Version XM_024993152.1
    Organism Bos taurus(cattle)
    Definition Bos taurus cyclin G associated kinase (GAK), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024993152.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGAGCTCT GCAGAGGGCA GCTGGTGGAA TTTTTAAAAA AAGTTGAATC GAAAGGTCCG 
    CTCTCGTGCG ACACCGTCCT GAAGATCTTC TACCAGACGT GCCGGGCGGT GCAGCACATG
    CACAGACAGA AGCCGCCCAT CATCCACAGG GACCTCAAGG TTGAAAACTT ACTGCTTAGT
    AACCAGGGGA CCATCAAGCT GTGTGACTTC GGCAGTGCCA CGACCATCTC ACACTACCCA
    GACTACAGCT GGAGCGCCCA GCGCCGGGCC CTGGTGGAGG AGGAGATCAC CAGGAACACG
    ACCCCCATGT ACAGGACGCC GGAAATTGTG GACTTGTACT CAAACTTTCC GATTGGCGAG
    AAGCAGGACA TCTGGGCCCT GGGCTGCATC CTGTACCTGC TCTGCTTCGG GCAGCACCCC
    TTTGAGGACG GAGCGAAGCT TCGCATCGTC AACGGGAAGT ACGCAATCCC TGCCGACGAC
    ACGCGGTACT CCGTGTTCCA CAGCCTCATC CGTTCCATGC TCAAGGTCAA CCCGGAGGAG
    CGGCTGTCCG TCACCGAGCT GGTCAACCAG CTCCAGGAGA TCGCGGCCGC CCGCAACGTG
    AACCCCAAGT CACCCATCAC TGAGCTCCTG GAGCAGAATG GAGGCTATGG GCACGCGGCC
    CCTCCCGGCT CCACGGGCAG CAGTCTGGTG GTCACTGAGT ACGAGCAGCC CTATGGCGGG
    TTCCTGGACA TTCTGCGTGG GGGCACCGAG CGCCTCTTCA CCAACATCAA GGACACCTCC
    TCCAAAGTCA TCCAGTCCGT TGCCAACTAT GCGAAGGGAG ATCTGGATAT TTCTTACATC
    ACGTCTAGAA TCGCAGTCAT GTCGTTCCCG GCGGAGGGCG TGGAGTCGGC CATCAAGAAC
    AACATTGAGG ATGTACGGCT GTTCCTGGAC TCGAAGCACC CGGGCCGCTA CGCCGTCTAC
    AACCTGTCCC CGCGGACCTA CCGGCCCTCG AAGTTCCACA ACCGGGTCTC CGACTGCGGC
    TGGGTGGCCC GGCGGGCCCC GAGCCTCCGC AGCCTGTACA GCCTCTGCAG GAACATGCAC
    GCCTGGCTGC GCCGCGACCC TGGCAACGTC TGCGTGGTGC ACTGCATGGA CGGGAGGGCG
    GTGTCGGCCG TGGCCGTGTG CTCCTTCTTG TGCTTCTGCC GGCTCTTCAG CACCGCGGAG
    GCCGCTGTGT ACATGTTCAG CATGAAGCGG TGCCCACCGG GCATCTGGCC ATCCCACAAG
    AGGTACATTG AGTACATGTG TGACATGGTG GCCGAGGAGC CCGTCACGCC CCACAGCAAG
    CCCATCCTGG TGAAGGCCGT GGTCATGACG CCTGTGCCGC TCTTCAGCAA GCAGAGGAGC
    GGCTGCAGGC CCTTCTGCGA GGTCTACGTG GGGGATGAGC GCGTGACCAC CACGTCCCAG
    GAATACGACA AGATGAGAGA CTTTAAAATT GAGGATGGCA AAGCGGTCAT TCCCCTGGGC
    ATAACAGTCC AGGGGGACGT GCTCATCATC ATCTATCATG CGAGGTCCAC CCTGGGAGGA
    AGGCTGCAGG CCAAGATGGC GTCCATGAAG ATGTTTCAGA TCCAGTTTCA CACAGGGTTC
    GTGCCTCGGA ACGCAGCTAC TGTGAAGTTT GCCAAGTATG ATCTGGATGC TTGCGACATC
    CAGGAGAAGT ACCCCGACTT GTTCCAAGTG AGCCTGGAGG TGGAGGTGGA GCCCAGAGAC
    AGGCCCGGCC ACGAGGCCCC GCCCTGGGAG GGTGCAGGCA TGCGAGGGCT GAACCCCAAG
    ATCCTCTTCT CCAGCCGGGA GGAGCAGCAA GATGTCCTGT CCAAGTTTGG GAAGCCAGAG
    CTCCCCAGGC AGCCTGGGTC CACCGCACAG TACGACGCCG AGGCCGGGCC CGCGGATGCC
    GAGCCCCCCG AGTCAGACTC GCCGCACAGC AGCGTGGATG CTGACCACTT CCTGCACACG
    CTGGACTGGC GGGAGGATCG GGACTCCAGT GTGGGCCCGG AGGGTCACCT GGCCGAGGAG
    GGTCCCTCTG CTGTGGCCGA GGACGGCTGT GTGAGTGAGC CATCGGACGA GGAAGCCCCG
    ACACCCTCTG CGGAGAGCGG GGACACAGGC GATGAGGACG TGCCCGCCCC CGCCGCCCCG
    CAGGACGACA GCGTGGACCT TCTGGGGCTG CACGCGGAGG CGGGGCCTGT GCCCGCCCCC
    CAGGCCCCCG GCGGCCCCCC CAGCAATGCA GACCTGCTCG GCTGCCTGCT CGGGGCCCCG
    GGCCCCGCTC CGGAGGGTGC TGTGGGGGGC CTGCTCGACG GGGATGCGGC CCTGGTCTTC
    AGCAGCCCAG CCCCCGCGCC TTGCACCCAG ACAACTGCCC CGGCCGCTGA CCCGTTCGGC
    CCGCTCCTGC TGCCGCCCGA CGTGGACACC CCTCCTAGCT CGCAGCCTGA CCTCTTCGGG
    GAATTCCTCA ACTCAGACGC CCCGGCCGCC CAGCCCGCGC CCTTCCCCTC CACCCACAGC
    GCTCCGCCGC CAGCCTGCAG CACCGACTTC CTGCATCTTG GGGACCTGCC CGCCGAGCCC
    AGCAAGATGA CAGCCTCTGC CAGCCACCCC GACCTGCTGG GAGCCTGGGA TGCCTGGGCT
    GAGGCGCCGG CCCCCGCGCT GGCTGCAGAC GGCACCTTCT TCTCTCCTGG AGACCAGCCA
    GCCCCTTCTG GCCTTCCGGC TGGCCAGACC AAGTCTCAGA GCCAGGACCC GTTTGCAGAC
    CTTGGCGACC TCAGTGCCGG CCTCCAAGGC TCGCCTGCCG CAGCTGCTCC TGGGGGCTTT
    GGTCCCAAAG CAGCGCCCCC TCCCAAGGCC AGCAGCTCCT GGCAGACGAG TCGGCCCCCA
    GCCCAGGGCA CCCCGTGGCC CCCCCAGGCC AAGCCGCCCC CCAAAGCCAG TGTACCGCCA
    AGGCCTAACT ACACCTCCAG CTTCAGTGTG ATCGGTAGCC GGGAGGAGCG GGGGGTCCGC
    GCCCCCAGCT TTGGTCCAAA GCCGAGGGTG TCAGAGAACG ACTTTGAAGA CCTGCTCTCC
    AACCAGGGCT TCTCCTCGCG GGCTGACAGG AAGGGGCCGC GGACCATCGC TGAGATGAGG
    AGGCAGGACC AGGCCCGGGA CTTGGATCCG CTGAAGCTGA AGCTTCTGGA GTGGACGGAG
    GGCAAGGAGA GGAACATCCG CGCCCTGCTG TCCACGCTGC ACACGGCGCT GTGGGACGGC
    GAGAGCCGCT GGACGCCGGT GGGCATGGCC GACCTGGTGA CGCCCGCACA AGTGAAGAAG
    CACTACCGCC GTGCCGTGCT GGTGGTCCAC CCCGACAAGG CCCGGGGGCA GCCCTACGAG
    CAGTATGCCC GGATGATCTT CATGGAGCTC AACGATGCGT GGGCTGAGTT TGAGAGCCAG
    GGCTCCCGGC CACTCTTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa235258
    Clone ID Related Accession (Same CDS sequence) XM_005208283.2 , XM_024993152.1 , XM_024993153.1
    Accession Version XM_024993153.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3441bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product cyclin-G-associated kinase isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGAGCTCT GCAGAGGGCA GCTGGTGGAA TTTTTAAAAA AAGTTGAATC GAAAGGTCCG 
    CTCTCGTGCG ACACCGTCCT GAAGATCTTC TACCAGACGT GCCGGGCGGT GCAGCACATG
    CACAGACAGA AGCCGCCCAT CATCCACAGG GACCTCAAGG TTGAAAACTT ACTGCTTAGT
    AACCAGGGGA CCATCAAGCT GTGTGACTTC GGCAGTGCCA CGACCATCTC ACACTACCCA
    GACTACAGCT GGAGCGCCCA GCGCCGGGCC CTGGTGGAGG AGGAGATCAC CAGGAACACG
    ACCCCCATGT ACAGGACGCC GGAAATTGTG GACTTGTACT CAAACTTTCC GATTGGCGAG
    AAGCAGGACA TCTGGGCCCT GGGCTGCATC CTGTACCTGC TCTGCTTCGG GCAGCACCCC
    TTTGAGGACG GAGCGAAGCT TCGCATCGTC AACGGGAAGT ACGCAATCCC TGCCGACGAC
    ACGCGGTACT CCGTGTTCCA CAGCCTCATC CGTTCCATGC TCAAGGTCAA CCCGGAGGAG
    CGGCTGTCCG TCACCGAGCT GGTCAACCAG CTCCAGGAGA TCGCGGCCGC CCGCAACGTG
    AACCCCAAGT CACCCATCAC TGAGCTCCTG GAGCAGAATG GAGGCTATGG GCACGCGGCC
    CCTCCCGGCT CCACGGGCAG CAGTCTGGTG GTCACTGAGT ACGAGCAGCC CTATGGCGGG
    TTCCTGGACA TTCTGCGTGG GGGCACCGAG CGCCTCTTCA CCAACATCAA GGACACCTCC
    TCCAAAGTCA TCCAGTCCGT TGCCAACTAT GCGAAGGGAG ATCTGGATAT TTCTTACATC
    ACGTCTAGAA TCGCAGTCAT GTCGTTCCCG GCGGAGGGCG TGGAGTCGGC CATCAAGAAC
    AACATTGAGG ATGTACGGCT GTTCCTGGAC TCGAAGCACC CGGGCCGCTA CGCCGTCTAC
    AACCTGTCCC CGCGGACCTA CCGGCCCTCG AAGTTCCACA ACCGGGTCTC CGACTGCGGC
    TGGGTGGCCC GGCGGGCCCC GAGCCTCCGC AGCCTGTACA GCCTCTGCAG GAACATGCAC
    GCCTGGCTGC GCCGCGACCC TGGCAACGTC TGCGTGGTGC ACTGCATGGA CGGGAGGGCG
    GTGTCGGCCG TGGCCGTGTG CTCCTTCTTG TGCTTCTGCC GGCTCTTCAG CACCGCGGAG
    GCCGCTGTGT ACATGTTCAG CATGAAGCGG TGCCCACCGG GCATCTGGCC ATCCCACAAG
    AGGTACATTG AGTACATGTG TGACATGGTG GCCGAGGAGC CCGTCACGCC CCACAGCAAG
    CCCATCCTGG TGAAGGCCGT GGTCATGACG CCTGTGCCGC TCTTCAGCAA GCAGAGGAGC
    GGCTGCAGGC CCTTCTGCGA GGTCTACGTG GGGGATGAGC GCGTGACCAC CACGTCCCAG
    GAATACGACA AGATGAGAGA CTTTAAAATT GAGGATGGCA AAGCGGTCAT TCCCCTGGGC
    ATAACAGTCC AGGGGGACGT GCTCATCATC ATCTATCATG CGAGGTCCAC CCTGGGAGGA
    AGGCTGCAGG CCAAGATGGC GTCCATGAAG ATGTTTCAGA TCCAGTTTCA CACAGGGTTC
    GTGCCTCGGA ACGCAGCTAC TGTGAAGTTT GCCAAGTATG ATCTGGATGC TTGCGACATC
    CAGGAGAAGT ACCCCGACTT GTTCCAAGTG AGCCTGGAGG TGGAGGTGGA GCCCAGAGAC
    AGGCCCGGCC ACGAGGCCCC GCCCTGGGAG GGTGCAGGCA TGCGAGGGCT GAACCCCAAG
    ATCCTCTTCT CCAGCCGGGA GGAGCAGCAA GATGTCCTGT CCAAGTTTGG GAAGCCAGAG
    CTCCCCAGGC AGCCTGGGTC CACCGCACAG TACGACGCCG AGGCCGGGCC CGCGGATGCC
    GAGCCCCCCG AGTCAGACTC GCCGCACAGC AGCGTGGATG CTGACCACTT CCTGCACACG
    CTGGACTGGC GGGAGGATCG GGACTCCAGT GTGGGCCCGG AGGGTCACCT GGCCGAGGAG
    GGTCCCTCTG CTGTGGCCGA GGACGGCTGT GTGAGTGAGC CATCGGACGA GGAAGCCCCG
    ACACCCTCTG CGGAGAGCGG GGACACAGGC GATGAGGACG TGCCCGCCCC CGCCGCCCCG
    CAGGACGACA GCGTGGACCT TCTGGGGCTG CACGCGGAGG CGGGGCCTGT GCCCGCCCCC
    CAGGCCCCCG GCGGCCCCCC CAGCAATGCA GACCTGCTCG GCTGCCTGCT CGGGGCCCCG
    GGCCCCGCTC CGGAGGGTGC TGTGGGGGGC CTGCTCGACG GGGATGCGGC CCTGGTCTTC
    AGCAGCCCAG CCCCCGCGCC TTGCACCCAG ACAACTGCCC CGGCCGCTGA CCCGTTCGGC
    CCGCTCCTGC TGCCGCCCGA CGTGGACACC CCTCCTAGCT CGCAGCCTGA CCTCTTCGGG
    GAATTCCTCA ACTCAGACGC CCCGGCCGCC CAGCCCGCGC CCTTCCCCTC CACCCACAGC
    GCTCCGCCGC CAGCCTGCAG CACCGACTTC CTGCATCTTG GGGACCTGCC CGCCGAGCCC
    AGCAAGATGA CAGCCTCTGC CAGCCACCCC GACCTGCTGG GAGCCTGGGA TGCCTGGGCT
    GAGGCGCCGG CCCCCGCGCT GGCTGCAGAC GGCACCTTCT TCTCTCCTGG AGACCAGCCA
    GCCCCTTCTG GCCTTCCGGC TGGCCAGACC AAGTCTCAGA GCCAGGACCC GTTTGCAGAC
    CTTGGCGACC TCAGTGCCGG CCTCCAAGGC TCGCCTGCCG CAGCTGCTCC TGGGGGCTTT
    GGTCCCAAAG CAGCGCCCCC TCCCAAGGCC AGCAGCTCCT GGCAGACGAG TCGGCCCCCA
    GCCCAGGGCA CCCCGTGGCC CCCCCAGGCC AAGCCGCCCC CCAAAGCCAG TGTACCGCCA
    AGGCCTAACT ACACCTCCAG CTTCAGTGTG ATCGGTAGCC GGGAGGAGCG GGGGGTCCGC
    GCCCCCAGCT TTGGTCCAAA GCCGAGGGTG TCAGAGAACG ACTTTGAAGA CCTGCTCTCC
    AACCAGGGCT TCTCCTCGCG GGCTGACAGG AAGGGGCCGC GGACCATCGC TGAGATGAGG
    AGGCAGGACC AGGCCCGGGA CTTGGATCCG CTGAAGCTGA AGCTTCTGGA GTGGACGGAG
    GGCAAGGAGA GGAACATCCG CGCCCTGCTG TCCACGCTGC ACACGGCGCT GTGGGACGGC
    GAGAGCCGCT GGACGCCGGT GGGCATGGCC GACCTGGTGA CGCCCGCACA AGTGAAGAAG
    CACTACCGCC GTGCCGTGCT GGTGGTCCAC CCCGACAAGG CCCGGGGGCA GCCCTACGAG
    CAGTATGCCC GGATGATCTT CATGGAGCTC AACGATGCGT GGGCTGAGTT TGAGAGCCAG
    GGCTCCCGGC CACTCTTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024848921.1
    CDS786..4226
    Translation

    Target ORF information:

    RefSeq Version XM_024993153.1
    Organism Bos taurus(cattle)
    Definition Bos taurus cyclin G associated kinase (GAK), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024993153.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGAGCTCT GCAGAGGGCA GCTGGTGGAA TTTTTAAAAA AAGTTGAATC GAAAGGTCCG 
    CTCTCGTGCG ACACCGTCCT GAAGATCTTC TACCAGACGT GCCGGGCGGT GCAGCACATG
    CACAGACAGA AGCCGCCCAT CATCCACAGG GACCTCAAGG TTGAAAACTT ACTGCTTAGT
    AACCAGGGGA CCATCAAGCT GTGTGACTTC GGCAGTGCCA CGACCATCTC ACACTACCCA
    GACTACAGCT GGAGCGCCCA GCGCCGGGCC CTGGTGGAGG AGGAGATCAC CAGGAACACG
    ACCCCCATGT ACAGGACGCC GGAAATTGTG GACTTGTACT CAAACTTTCC GATTGGCGAG
    AAGCAGGACA TCTGGGCCCT GGGCTGCATC CTGTACCTGC TCTGCTTCGG GCAGCACCCC
    TTTGAGGACG GAGCGAAGCT TCGCATCGTC AACGGGAAGT ACGCAATCCC TGCCGACGAC
    ACGCGGTACT CCGTGTTCCA CAGCCTCATC CGTTCCATGC TCAAGGTCAA CCCGGAGGAG
    CGGCTGTCCG TCACCGAGCT GGTCAACCAG CTCCAGGAGA TCGCGGCCGC CCGCAACGTG
    AACCCCAAGT CACCCATCAC TGAGCTCCTG GAGCAGAATG GAGGCTATGG GCACGCGGCC
    CCTCCCGGCT CCACGGGCAG CAGTCTGGTG GTCACTGAGT ACGAGCAGCC CTATGGCGGG
    TTCCTGGACA TTCTGCGTGG GGGCACCGAG CGCCTCTTCA CCAACATCAA GGACACCTCC
    TCCAAAGTCA TCCAGTCCGT TGCCAACTAT GCGAAGGGAG ATCTGGATAT TTCTTACATC
    ACGTCTAGAA TCGCAGTCAT GTCGTTCCCG GCGGAGGGCG TGGAGTCGGC CATCAAGAAC
    AACATTGAGG ATGTACGGCT GTTCCTGGAC TCGAAGCACC CGGGCCGCTA CGCCGTCTAC
    AACCTGTCCC CGCGGACCTA CCGGCCCTCG AAGTTCCACA ACCGGGTCTC CGACTGCGGC
    TGGGTGGCCC GGCGGGCCCC GAGCCTCCGC AGCCTGTACA GCCTCTGCAG GAACATGCAC
    GCCTGGCTGC GCCGCGACCC TGGCAACGTC TGCGTGGTGC ACTGCATGGA CGGGAGGGCG
    GTGTCGGCCG TGGCCGTGTG CTCCTTCTTG TGCTTCTGCC GGCTCTTCAG CACCGCGGAG
    GCCGCTGTGT ACATGTTCAG CATGAAGCGG TGCCCACCGG GCATCTGGCC ATCCCACAAG
    AGGTACATTG AGTACATGTG TGACATGGTG GCCGAGGAGC CCGTCACGCC CCACAGCAAG
    CCCATCCTGG TGAAGGCCGT GGTCATGACG CCTGTGCCGC TCTTCAGCAA GCAGAGGAGC
    GGCTGCAGGC CCTTCTGCGA GGTCTACGTG GGGGATGAGC GCGTGACCAC CACGTCCCAG
    GAATACGACA AGATGAGAGA CTTTAAAATT GAGGATGGCA AAGCGGTCAT TCCCCTGGGC
    ATAACAGTCC AGGGGGACGT GCTCATCATC ATCTATCATG CGAGGTCCAC CCTGGGAGGA
    AGGCTGCAGG CCAAGATGGC GTCCATGAAG ATGTTTCAGA TCCAGTTTCA CACAGGGTTC
    GTGCCTCGGA ACGCAGCTAC TGTGAAGTTT GCCAAGTATG ATCTGGATGC TTGCGACATC
    CAGGAGAAGT ACCCCGACTT GTTCCAAGTG AGCCTGGAGG TGGAGGTGGA GCCCAGAGAC
    AGGCCCGGCC ACGAGGCCCC GCCCTGGGAG GGTGCAGGCA TGCGAGGGCT GAACCCCAAG
    ATCCTCTTCT CCAGCCGGGA GGAGCAGCAA GATGTCCTGT CCAAGTTTGG GAAGCCAGAG
    CTCCCCAGGC AGCCTGGGTC CACCGCACAG TACGACGCCG AGGCCGGGCC CGCGGATGCC
    GAGCCCCCCG AGTCAGACTC GCCGCACAGC AGCGTGGATG CTGACCACTT CCTGCACACG
    CTGGACTGGC GGGAGGATCG GGACTCCAGT GTGGGCCCGG AGGGTCACCT GGCCGAGGAG
    GGTCCCTCTG CTGTGGCCGA GGACGGCTGT GTGAGTGAGC CATCGGACGA GGAAGCCCCG
    ACACCCTCTG CGGAGAGCGG GGACACAGGC GATGAGGACG TGCCCGCCCC CGCCGCCCCG
    CAGGACGACA GCGTGGACCT TCTGGGGCTG CACGCGGAGG CGGGGCCTGT GCCCGCCCCC
    CAGGCCCCCG GCGGCCCCCC CAGCAATGCA GACCTGCTCG GCTGCCTGCT CGGGGCCCCG
    GGCCCCGCTC CGGAGGGTGC TGTGGGGGGC CTGCTCGACG GGGATGCGGC CCTGGTCTTC
    AGCAGCCCAG CCCCCGCGCC TTGCACCCAG ACAACTGCCC CGGCCGCTGA CCCGTTCGGC
    CCGCTCCTGC TGCCGCCCGA CGTGGACACC CCTCCTAGCT CGCAGCCTGA CCTCTTCGGG
    GAATTCCTCA ACTCAGACGC CCCGGCCGCC CAGCCCGCGC CCTTCCCCTC CACCCACAGC
    GCTCCGCCGC CAGCCTGCAG CACCGACTTC CTGCATCTTG GGGACCTGCC CGCCGAGCCC
    AGCAAGATGA CAGCCTCTGC CAGCCACCCC GACCTGCTGG GAGCCTGGGA TGCCTGGGCT
    GAGGCGCCGG CCCCCGCGCT GGCTGCAGAC GGCACCTTCT TCTCTCCTGG AGACCAGCCA
    GCCCCTTCTG GCCTTCCGGC TGGCCAGACC AAGTCTCAGA GCCAGGACCC GTTTGCAGAC
    CTTGGCGACC TCAGTGCCGG CCTCCAAGGC TCGCCTGCCG CAGCTGCTCC TGGGGGCTTT
    GGTCCCAAAG CAGCGCCCCC TCCCAAGGCC AGCAGCTCCT GGCAGACGAG TCGGCCCCCA
    GCCCAGGGCA CCCCGTGGCC CCCCCAGGCC AAGCCGCCCC CCAAAGCCAG TGTACCGCCA
    AGGCCTAACT ACACCTCCAG CTTCAGTGTG ATCGGTAGCC GGGAGGAGCG GGGGGTCCGC
    GCCCCCAGCT TTGGTCCAAA GCCGAGGGTG TCAGAGAACG ACTTTGAAGA CCTGCTCTCC
    AACCAGGGCT TCTCCTCGCG GGCTGACAGG AAGGGGCCGC GGACCATCGC TGAGATGAGG
    AGGCAGGACC AGGCCCGGGA CTTGGATCCG CTGAAGCTGA AGCTTCTGGA GTGGACGGAG
    GGCAAGGAGA GGAACATCCG CGCCCTGCTG TCCACGCTGC ACACGGCGCT GTGGGACGGC
    GAGAGCCGCT GGACGCCGGT GGGCATGGCC GACCTGGTGA CGCCCGCACA AGTGAAGAAG
    CACTACCGCC GTGCCGTGCT GGTGGTCCAC CCCGACAAGG CCCGGGGGCA GCCCTACGAG
    CAGTATGCCC GGATGATCTT CATGGAGCTC AACGATGCGT GGGCTGAGTT TGAGAGCCAG
    GGCTCCCGGC CACTCTTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa235259
    Clone ID Related Accession (Same CDS sequence) XM_010806359.3
    Accession Version XM_010806359.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3417bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product cyclin-G-associated kinase isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010806359.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGTCGCTGC TGCAGTCCGC GCTCGACTTC CTGGCGGGCC CGGGCTCCCT GGGGGGCGCG 
    GCCGGCCGCG ACCAGACGGA CTTCGTGGGG CAGACGGTGG AGATGGGCGA GCTGCGGCTG
    CGGGTGCGCC GGGTCCTGGC TGAAGGAGGG TTTGCATTTG TGTATGAAGC TCAAGATCTG
    GGAAGTGGCA GAGAGTATGC ATTGAAGAGA CTGCTGTCCA ACGAAGAGGA GAAGAACAGA
    GCCATCATTC AGGAAGTGTG TTTCATGAAA AAACTTTCAG GCCACCCAAA CATTGTCCAG
    TTTTGTTCTG CAGCATCGAT AGGAAAAGAG GAGTCGGACA CTGGGCAGGC GGAGTTCCTG
    CTGCTCATGG AGCTCTGCAG AGGGCAGCTG GTGGAATTTT TAAAAAAAGT TGAATCGAAA
    GGTCCGCTCT CGTGCGACAC CGTCCTGAAG ATCTTCTACC AGACGTGCCG GGCGGTGCAG
    CACATGCACA GACAGAAGCC GCCCATCATC CACAGGGACC TCAAGGTTGA AAACTTACTG
    CTTAGTAACC AGGGGACCAT CAAGCTGTGT GACTTCGGCA GTGCCACGAC CATCTCACAC
    TACCCAGACT ACAGCTGGAG CGCCCAGCGC CGGGCCCTGG TGGAGGAGGA GATCACCAGG
    AACACGACCC CCATGTACAG GACGCCGGAA ATTGTGGACT TGTACTCAAA CTTTCCGATT
    GGCGAGAAGC AGGACATCTG GGCCCTGGGC TGCATCCTGT ACCTGCTCTG CTTCGGGCAG
    CACCCCTTTG AGGACGGAGC GAAGCTTCGC ATCGTCAACG GGAAGTACGC AATCCCTGCC
    GACGACACGC GGTACTCCGT GTTCCACAGC CTCATCCGTT CCATGCTCAA GGTCAACCCG
    GAGGAGCGGC TGTCCGTCAC CGAGCTGGTC AACCAGCTCC AGGAGATCGC GGCCGCCCGC
    AACGTGAACC CCAAGTCACC CATCACTGAG CTCCTGGAGC AGAATGGAGG CTATGGGCAC
    GCGGCCCCTC CCGGCTCCAC GGGCAGCAGT CTGGTGGTCA CTGAGTACGA GCAGCCCTAT
    GGCGGGTTCC TGGACATTCT GCGTGGGGGC ACCGAGCGCC TCTTCACCAA CATCAAGGAC
    ACCTCCTCCA AAGTCATCCA GTCCGTTGCC AACTATGCGA AGGGAGATCT GGATATTTCT
    TACATCACGT CTAGAATCGC AGTCATGTCG TTCCCGGCGG AGGGCGTGGA GTCGGCCATC
    AAGAACAACA TTGAGGATGT ACGGCTGTTC CTGGACTCGA AGCACCCGGG CCGCTACGCC
    GTCTACAACC TGTCCCCGCG GACCTACCGG CCCTCGAAGT TCCACAACCG GGTCTCCGAC
    TGCGGCTGGG TGGCCCGGCG GGCCCCGAGC CTCCGCAGCC TGTACAGCCT CTGCAGGAAC
    ATGCACGCCT GGCTGCGCCG CGACCCTGGC AACGTCTGCG TGGTGCACTG CATGGACGGG
    AGGGCGGTGT CGGCCGTGGC CGTGTGCTCC TTCTTGTGCT TCTGCCGGCT CTTCAGCACC
    GCGGAGGCCG CTGTGTACAT GTTCAGCATG AAGCGGTGCC CACCGGGCAT CTGGCCATCC
    CACAAGAGGT ACATTGAGTA CATGTGTGAC ATGGTGGCCG AGGAGCCCGT CACGCCCCAC
    AGCAAGCCCA TCCTGGTGAA GGCCGTGGTC ATGACGCCTG TGCCGCTCTT CAGCAAGCAG
    AGGAGCGGCT GCAGGCCCTT CTGCGAGGTC TACGTGGGGG ATGAGCGCGT GACCACCACG
    TCCCAGGAAT ACGACAAGAT GAGAGACTTT AAAATTGAGG ATGGCAAAGC GGTCATTCCC
    CTGGGCATAA CAGTCCAGGG GGACGTGCTC ATCATCATCT ATCATGCGAG GTCCACCCTG
    GGAGGAAGGC TGCAGGCCAA GATGGCGTCC ATGAAGATGT TTCAGATCCA GTTTCACACA
    GGGTTCGTGC CTCGGAACGC AGCTACTGTG AAGTTTGCCA AGTATGATCT GGATGCTTGC
    GACATCCAGG AGAAGTACCC CGACTTGTTC CAAGTGAGCC TGGAGGTGGA GGTGGAGCCC
    AGAGACAGGC CCGGCCACGA GGCCCCGCCC TGGGAGGGTG CAGGCATGCG AGGGCTGAAC
    CCCAAGATCC TCTTCTCCAG CCGGGAGGAG CAGCAAGATG TCCTGTCCAA GTTTGGGAAG
    CCAGAGCTCC CCAGGCAGCC TGGGTCCACC GCACAGTACG ACGCCGAGGC CGGGCCCGCG
    GATGCCGAGC CCCCCGAGTC AGACTCGCCG CACAGCAGCG TGGATGCTGA CCACTTCCTG
    CACACGCTGG ACTGGCGGGA GGATCGGGAC TCCAGTGTGG GCCCGGAGGG TCACCTGGCC
    GAGGAGGGTC CCTCTGCTGT GGCCGAGGAC GGCTGTGTGA GTGAGCCATC GGACGAGGAA
    GCCCCGACAC CCTCTGCGGA GAGCGGGGAC ACAGGCGATG AGGACGTGCC CGCCCCCGCC
    GCCCCGCAGG ACGACAGCGT GGACCTTCTG GGGCTGCACG CGGAGGCGGG GCCTGTGCCC
    GCCCCCCAGG CCCCCGGCGG CCCCCCCAGC AATGCAGACC TGCTCGGCTG CCTGCTCGGG
    GCCCCGGGCC CCGCTCCGGA GGGTGCTGTG GGGGGCCTGC TCGACGGGGA TGCGGCCCTG
    GTCTTCAGCA GCCCAGCCCC CGCGCCTTGC ACCCAGACAA CTGCCCCGGC CGCTGACCCG
    TTCGGCCCGC TCCTGCTGCC GCCCGACGTG GACACCCCTC CTAGCTCGCA GCCTGACCTC
    TTCGGGGAAT TCCTCAACTC AGACGCCCCG GCCGCCCAGC CCGCGCCCTT CCCCTCCACC
    CACAGCGCTC CGCCGCCAGC CTGCAGCACC GACTTCCTGC ATCTTGGGGA CCTGCCCGCC
    GAGCCCAGCA AGATGACAGC CTCTGCCAGC CACCCCGACC TGCTGGGAGC CTGGGATGCC
    TGGGCTGAGG CGCCGGCCCC CGCGCTGGCT GCAGACGGCA CCTTCTTCTC TCCTGGAGAC
    CAGCCAGCCC CTTCTGGCCT TCCGGCTGGC CAGACCAAGT CTCAGAGCCA GGACCCGTTT
    GCAGACCTTG GCGACCTCAG TGCCGGCCTC CAAGGGCCCA GCAACAGCAG CAGATGCCCC
    TCTGCCTTCT CTCAGGAGCT TGTTGCCAAG ACAACGCCAG GAGCTGCCAG AGCAGGCACA
    GGCACTGGCT CCTCTGCCAG CTCCATGGAG TGTGGGGGCC AGCTGGGGGC TGCCTCAGCC
    CAGGATGGTG GAACCTGCCT GCCCCGCTTG GAGTGTGGGG CCGGGGTGCA GAATTCACCA
    CCATCACTGT CCTTTCTGAG AAGCCCTCTA AGGACGTTGG TTCTTTGGAG GCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010804661.2
    CDS114..3530
    Translation

    Target ORF information:

    RefSeq Version XM_010806359.3
    Organism Bos taurus(cattle)
    Definition Bos taurus cyclin G associated kinase (GAK), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806359.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGTCGCTGC TGCAGTCCGC GCTCGACTTC CTGGCGGGCC CGGGCTCCCT GGGGGGCGCG 
    GCCGGCCGCG ACCAGACGGA CTTCGTGGGG CAGACGGTGG AGATGGGCGA GCTGCGGCTG
    CGGGTGCGCC GGGTCCTGGC TGAAGGAGGG TTTGCATTTG TGTATGAAGC TCAAGATCTG
    GGAAGTGGCA GAGAGTATGC ATTGAAGAGA CTGCTGTCCA ACGAAGAGGA GAAGAACAGA
    GCCATCATTC AGGAAGTGTG TTTCATGAAA AAACTTTCAG GCCACCCAAA CATTGTCCAG
    TTTTGTTCTG CAGCATCGAT AGGAAAAGAG GAGTCGGACA CTGGGCAGGC GGAGTTCCTG
    CTGCTCATGG AGCTCTGCAG AGGGCAGCTG GTGGAATTTT TAAAAAAAGT TGAATCGAAA
    GGTCCGCTCT CGTGCGACAC CGTCCTGAAG ATCTTCTACC AGACGTGCCG GGCGGTGCAG
    CACATGCACA GACAGAAGCC GCCCATCATC CACAGGGACC TCAAGGTTGA AAACTTACTG
    CTTAGTAACC AGGGGACCAT CAAGCTGTGT GACTTCGGCA GTGCCACGAC CATCTCACAC
    TACCCAGACT ACAGCTGGAG CGCCCAGCGC CGGGCCCTGG TGGAGGAGGA GATCACCAGG
    AACACGACCC CCATGTACAG GACGCCGGAA ATTGTGGACT TGTACTCAAA CTTTCCGATT
    GGCGAGAAGC AGGACATCTG GGCCCTGGGC TGCATCCTGT ACCTGCTCTG CTTCGGGCAG
    CACCCCTTTG AGGACGGAGC GAAGCTTCGC ATCGTCAACG GGAAGTACGC AATCCCTGCC
    GACGACACGC GGTACTCCGT GTTCCACAGC CTCATCCGTT CCATGCTCAA GGTCAACCCG
    GAGGAGCGGC TGTCCGTCAC CGAGCTGGTC AACCAGCTCC AGGAGATCGC GGCCGCCCGC
    AACGTGAACC CCAAGTCACC CATCACTGAG CTCCTGGAGC AGAATGGAGG CTATGGGCAC
    GCGGCCCCTC CCGGCTCCAC GGGCAGCAGT CTGGTGGTCA CTGAGTACGA GCAGCCCTAT
    GGCGGGTTCC TGGACATTCT GCGTGGGGGC ACCGAGCGCC TCTTCACCAA CATCAAGGAC
    ACCTCCTCCA AAGTCATCCA GTCCGTTGCC AACTATGCGA AGGGAGATCT GGATATTTCT
    TACATCACGT CTAGAATCGC AGTCATGTCG TTCCCGGCGG AGGGCGTGGA GTCGGCCATC
    AAGAACAACA TTGAGGATGT ACGGCTGTTC CTGGACTCGA AGCACCCGGG CCGCTACGCC
    GTCTACAACC TGTCCCCGCG GACCTACCGG CCCTCGAAGT TCCACAACCG GGTCTCCGAC
    TGCGGCTGGG TGGCCCGGCG GGCCCCGAGC CTCCGCAGCC TGTACAGCCT CTGCAGGAAC
    ATGCACGCCT GGCTGCGCCG CGACCCTGGC AACGTCTGCG TGGTGCACTG CATGGACGGG
    AGGGCGGTGT CGGCCGTGGC CGTGTGCTCC TTCTTGTGCT TCTGCCGGCT CTTCAGCACC
    GCGGAGGCCG CTGTGTACAT GTTCAGCATG AAGCGGTGCC CACCGGGCAT CTGGCCATCC
    CACAAGAGGT ACATTGAGTA CATGTGTGAC ATGGTGGCCG AGGAGCCCGT CACGCCCCAC
    AGCAAGCCCA TCCTGGTGAA GGCCGTGGTC ATGACGCCTG TGCCGCTCTT CAGCAAGCAG
    AGGAGCGGCT GCAGGCCCTT CTGCGAGGTC TACGTGGGGG ATGAGCGCGT GACCACCACG
    TCCCAGGAAT ACGACAAGAT GAGAGACTTT AAAATTGAGG ATGGCAAAGC GGTCATTCCC
    CTGGGCATAA CAGTCCAGGG GGACGTGCTC ATCATCATCT ATCATGCGAG GTCCACCCTG
    GGAGGAAGGC TGCAGGCCAA GATGGCGTCC ATGAAGATGT TTCAGATCCA GTTTCACACA
    GGGTTCGTGC CTCGGAACGC AGCTACTGTG AAGTTTGCCA AGTATGATCT GGATGCTTGC
    GACATCCAGG AGAAGTACCC CGACTTGTTC CAAGTGAGCC TGGAGGTGGA GGTGGAGCCC
    AGAGACAGGC CCGGCCACGA GGCCCCGCCC TGGGAGGGTG CAGGCATGCG AGGGCTGAAC
    CCCAAGATCC TCTTCTCCAG CCGGGAGGAG CAGCAAGATG TCCTGTCCAA GTTTGGGAAG
    CCAGAGCTCC CCAGGCAGCC TGGGTCCACC GCACAGTACG ACGCCGAGGC CGGGCCCGCG
    GATGCCGAGC CCCCCGAGTC AGACTCGCCG CACAGCAGCG TGGATGCTGA CCACTTCCTG
    CACACGCTGG ACTGGCGGGA GGATCGGGAC TCCAGTGTGG GCCCGGAGGG TCACCTGGCC
    GAGGAGGGTC CCTCTGCTGT GGCCGAGGAC GGCTGTGTGA GTGAGCCATC GGACGAGGAA
    GCCCCGACAC CCTCTGCGGA GAGCGGGGAC ACAGGCGATG AGGACGTGCC CGCCCCCGCC
    GCCCCGCAGG ACGACAGCGT GGACCTTCTG GGGCTGCACG CGGAGGCGGG GCCTGTGCCC
    GCCCCCCAGG CCCCCGGCGG CCCCCCCAGC AATGCAGACC TGCTCGGCTG CCTGCTCGGG
    GCCCCGGGCC CCGCTCCGGA GGGTGCTGTG GGGGGCCTGC TCGACGGGGA TGCGGCCCTG
    GTCTTCAGCA GCCCAGCCCC CGCGCCTTGC ACCCAGACAA CTGCCCCGGC CGCTGACCCG
    TTCGGCCCGC TCCTGCTGCC GCCCGACGTG GACACCCCTC CTAGCTCGCA GCCTGACCTC
    TTCGGGGAAT TCCTCAACTC AGACGCCCCG GCCGCCCAGC CCGCGCCCTT CCCCTCCACC
    CACAGCGCTC CGCCGCCAGC CTGCAGCACC GACTTCCTGC ATCTTGGGGA CCTGCCCGCC
    GAGCCCAGCA AGATGACAGC CTCTGCCAGC CACCCCGACC TGCTGGGAGC CTGGGATGCC
    TGGGCTGAGG CGCCGGCCCC CGCGCTGGCT GCAGACGGCA CCTTCTTCTC TCCTGGAGAC
    CAGCCAGCCC CTTCTGGCCT TCCGGCTGGC CAGACCAAGT CTCAGAGCCA GGACCCGTTT
    GCAGACCTTG GCGACCTCAG TGCCGGCCTC CAAGGGCCCA GCAACAGCAG CAGATGCCCC
    TCTGCCTTCT CTCAGGAGCT TGTTGCCAAG ACAACGCCAG GAGCTGCCAG AGCAGGCACA
    GGCACTGGCT CCTCTGCCAG CTCCATGGAG TGTGGGGGCC AGCTGGGGGC TGCCTCAGCC
    CAGGATGGTG GAACCTGCCT GCCCCGCTTG GAGTGTGGGG CCGGGGTGCA GAATTCACCA
    CCATCACTGT CCTTTCTGAG AAGCCCTCTA AGGACGTTGG TTCTTTGGAG GCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa40735
    Clone ID Related Accession (Same CDS sequence) NM_001046084.2
    Accession Version NM_001046084.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3807bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-13
    Organism Bos taurus(cattle)
    Product cyclin-G-associated kinase
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC105343.1. On Aug 31, 2012 this sequence version replaced NM_001046084.1. ##Evidence-Data-START## Transcript exon combination :: BC105343.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGTCGCTGC TGCAGTCCGC GCTCGACTTC CTGGCGGGCC CGGGCTCCCT GGGGGGCGCG 
    GCCGGCCGCG ACCAGACGGA CTTCGTGGGG CAGACGGTGG AGATGGGCGA GCTGCGGCTG
    CGGGTGCGCC GGGTCCTGGC TGAAGGAGGG TTTGCATTTG TGTATGAAGC TCAAGATCTG
    GGAAGTGGCA GAGAGTATGC ATTGAAGAGA CTGCTGTCCA ACGAAGAGGA GAAGAACAGA
    GCCATCATTC AGGAAGTGTG TTTCATGAAA AAACTTTCAG GCCACCCAAA CATTGTCCAG
    TTTTGTTCTG CAGCATCGAT AGGAAAAGAG GAGTCGGACA CTGGGCAGGC GGAGTTCCTG
    CTGCTCATGG AGCTCTGCAG AGGGCAGCTG GTGGAATTTT TAAAAAAAGT TGAATCGAAA
    GGTCCGCTCT CGTGCGACAC CGTCCTGAAG ATCTTCTACC AGACGTGCCG GGCGGTGCAG
    CACATGCACA GACAGAAGCC GCCCATCATC CACAGGGACC TCAAGGTTGA AAACTTACTG
    CTTAGTAACC AGGGGACCAT CAAGCTGTGT GACTTCGGCA GTGCCACGAC CATCTCACAC
    TACCCAGACT ACAGCTGGAG CGCCCAGCGC CGGGCCCTGG TGGAGGAGGA GATCACCAGG
    AACACGACCC CCATGTACAG GACGCCGGAA ATTGTGGACT TGTACTCAAA CTTTCCGATT
    GGCGAGAAGC AGGACATCTG GGCCCTGGGC TGCATCCTGT ACCTGCTCTG CTTCGGGCAG
    CACCCCTTTG AGGACGGAGC GAAGCTTCGC ATCGTCAACG GGAAGTACGC AATCCCTGCC
    GACGACACGC GGTACTCCGT GTTCCACAGC CTCATCCGTT CCATGCTCAA GGTCAACCCG
    GAGGAGCGGC TGTCCGTCAC CGAGCTGGTC AACCAGCTCC AGGAGATCGC GGCCGCCCGC
    AACGTGAACC CCAAGTCACC CATCACTGAG CTCCTGGAGC AGAATGGAGG CTATGGGCAC
    GCGGCCCCTC CCGGCTCCAC GGGCAGCAGT CTGGTGGTCA CTGAGTACGA GCAGCCCTAT
    GGCGGGTTCC TGGACATTCT GCGTGGGGGC ACCGAGCGCC TCTTCACCAA CATCAAGGAC
    ACCTCCTCCA AAGTCATCCA GTCCGTTGCC AACTATGCGA AGGGAGATCT GGATATTTCT
    TACATCACGT CTAGAATCGC AGTCATGTCG TTCCCGGCGG AGGGCGTGGA GTCGGCCATC
    AAGAACAACA TTGAGGATGT ACGGCTGTTC CTGGACTCGA AGCACCCGGG CCGCTACGCC
    GTCTACAACC TGTCCCCGCG GACCTACCGG CCCTCGAAGT TCCACAACCG GGTCTCCGAC
    TGCGGCTGGG TGGCCCGGCG GGCCCCGAGC CTCCGCAGCC TGTACAGCCT CTGCAGGAAC
    ATGCACGCCT GGCTGCGCCG CGACCCTGGC AACGTCTGCG TGGTGCACTG CATGGACGGG
    AGGGCGGTGT CGGCCGTGGC CGTGTGCTCC TTCTTGTGCT TCTGCCGGCT CTTCAGCACC
    GCGGAGGCCG CTGTGTACAT GTTCAGCATG AAGCGGTGCC CACCGGGCAT CTGGCCATCC
    CACAAGAGGT ACATTGAGTA CATGTGTGAC ATGGTGGCCG AGGAGCCCGT CACGCCCCAC
    AGCAAGCCCA TCCTGGTGAA GGCCGTGGTC ATGACGCCTG TGCCGCTCTT CAGCAAGCAG
    AGGAGCGGCT GCAGGCCCTT CTGCGAGGTC TACGTGGGGG ATGAGCGCGT GACCACCACG
    TCCCAGGAAT ACGACAAGAT GAGAGACTTT AAAATTGAGG ATGGCAAAGC GGTCATTCCC
    CTGGGCATAA CAGTCCAGGG GGACGTGCTC ATCATCATCT ATCATGCGAG GTCCACCCTG
    GGAGGAAGGC TGCAGGCCAA GATGGCGTCC ATGAAGATGT TTCAGATCCA GTTTCACACA
    GGGTTCGTGC CTCGGAACGC AGCTACTGTG AAGTTTGCCA AGTATGATCT GGATGCTTGC
    GACATCCAGG AGAAGTACCC CGACTTGTTC CAAGTGAGCC TGGAGGTGGA GGTGGAGCCC
    AGAGACAGGC CCGGCCACGA GGCCCCGCCC TGGGAGGGTG CAGGCATGCG AGGGCTGAAC
    CCCAAGATCC TCTTCTCCAG CCGGGAGGAG CAGCAAGATG TCCTGTCCAA GTTTGGGAAG
    CCAGAGCTCC CCAGGCAGCC TGGGTCCACC GCACAGTACG ACGCCGAGGC CGGGCCCGCG
    GATGCCGAGC CCCCCGAGTC AGACTCGCCG CACAGCAGCG TGGATGCTGA CCACTTCCTG
    CACACGCTGG ACTGGCGGGA GGATCGGGAC TCCAGTGTGG GCCCGGAGGG TCACCTGGCC
    GAGGAGGGTC CCTCTGCTGT GGCCGAGGAC GGCTGTGTGA GTGAGCCATC GGACGAGGAA
    GCCCCGACAC CCTCTGCGGA GAGCGGGGAC ACAGGCGATG AGGACGTGCC CGCCCCCGCC
    GCCCCGCAGG ACGACAGCGT GGACCTTCTG GGGCTGCACG CGGAGGCGGG GCCTGTGCCC
    GCCCCCCAGG CCCCCGGCGG CCCCCCCAGC AATGCAGACC TGCTCGGCTG CCTGCTCGGG
    GCCCCGGGCC CCGCTCCGGA GGGTGCTGTG GGGGGCCTGC TCGACGGGGA TGCGGCCCTG
    GTCTTCAGCA GCCCAGCCCC CGCGCCTTGC ACCCAGACAA CTGCCCCGGC CGCTGACCCG
    TTCGGCCCGC TCCTGCTGCC GCCCGACGTG GACACCCCTC CTAGCTCGCA GCCTGACCTC
    TTCGGGGAAT TCCTCAACTC AGACGCCCCG GCCGCCCAGC CCGCGCCCTT CCCCTCCACG
    CACAGCGCTC CGCCGCCAGC CTGCAGCACC GACTTCCTGC ATCTTGGGGA CCTGCCCGCC
    GAGCCCAGCA AGATGACAGC CTCTGCCAGC CACCCCGACC TGCTGGGAGC CTGGGATGCC
    TGGGCTGAGG CGCCGGCCCC CGCGCTGGCT GCAGACGGCA CCTTCTTCTC TCCTGGAGAC
    CAGCCAGCCC CTTCTGGCCT TCCGGCTGGC CAGACCAAGT CTCAGAGCCA GGACCCGTTT
    GCAGACCTTG GCGACCTCAG TGCCGGCCTC CAAGGCTCGC CTGCCGCAGC TGCTCCTGGG
    GGCTTTGGTC CCAAAGCAGC GCCCCCTCCC AAGGCCAGCA GCTCCTGGCA GACGAGTCGG
    CCCCCAGCCC AGGGCACCCC GTGGCCCCCC CAGGCCAAGC CGCCCCCCAA AGCCAGTGTA
    CCGCCAAGGC CTAACTACAC CTCCAGCTTC AGTGTGATCG GTAGCCGGGA GGAGCGGGGG
    GTCCGCGCCC CCAGCTTTGG TCCAAAGCCG AGGGTGTCAG AGAACGACTT TGAAGACCTG
    CTCTCCAACC AGGGCTTCTC CTCGCGGGCT GACAGGAAGG GGCCGCGGAC CATCGCTGAG
    ATGAGGAGGC AGGACCAGGC CCGGGACTCG GATCCGCTGA AGCTGAAGCT TCTGGAGTGG
    ACGGAGGGCA AGGAGAGGAA CATCCGCGCC CTGCTGTCCA CGCTGCACAC GGCGCTGTGG
    GATGGCGAGA GCCGCTGGAC GCCGGTGGGC ATGGCCGACC TGGTGACGCC CGCACAAGTG
    AAGAAGCACT ACCGCCGTGC CGTGCTGGTG GTCCACCCCG ACAAGGCCCG GGGGCAGCCC
    TACGAGCAGT ATGCCCGGAT GATCTTCATG GAGCTCAACG ATGCGTGGGC TGAGTTTGAG
    AGCCAGGGCT CCCGGCCACT CTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001039549.1
    CDS118..3924
    Translation

    Target ORF information:

    RefSeq Version NM_001046084.2
    Organism Bos taurus(cattle)
    Definition Bos taurus cyclin G associated kinase (GAK), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001046084.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGTCGCTGC TGCAGTCCGC GCTCGACTTC CTGGCGGGCC CGGGCTCCCT GGGGGGCGCG 
    GCCGGCCGCG ACCAGACGGA CTTCGTGGGG CAGACGGTGG AGATGGGCGA GCTGCGGCTG
    CGGGTGCGCC GGGTCCTGGC TGAAGGAGGG TTTGCATTTG TGTATGAAGC TCAAGATCTG
    GGAAGTGGCA GAGAGTATGC ATTGAAGAGA CTGCTGTCCA ACGAAGAGGA GAAGAACAGA
    GCCATCATTC AGGAAGTGTG TTTCATGAAA AAACTTTCAG GCCACCCAAA CATTGTCCAG
    TTTTGTTCTG CAGCATCGAT AGGAAAAGAG GAGTCGGACA CTGGGCAGGC GGAGTTCCTG
    CTGCTCATGG AGCTCTGCAG AGGGCAGCTG GTGGAATTTT TAAAAAAAGT TGAATCGAAA
    GGTCCGCTCT CGTGCGACAC CGTCCTGAAG ATCTTCTACC AGACGTGCCG GGCGGTGCAG
    CACATGCACA GACAGAAGCC GCCCATCATC CACAGGGACC TCAAGGTTGA AAACTTACTG
    CTTAGTAACC AGGGGACCAT CAAGCTGTGT GACTTCGGCA GTGCCACGAC CATCTCACAC
    TACCCAGACT ACAGCTGGAG CGCCCAGCGC CGGGCCCTGG TGGAGGAGGA GATCACCAGG
    AACACGACCC CCATGTACAG GACGCCGGAA ATTGTGGACT TGTACTCAAA CTTTCCGATT
    GGCGAGAAGC AGGACATCTG GGCCCTGGGC TGCATCCTGT ACCTGCTCTG CTTCGGGCAG
    CACCCCTTTG AGGACGGAGC GAAGCTTCGC ATCGTCAACG GGAAGTACGC AATCCCTGCC
    GACGACACGC GGTACTCCGT GTTCCACAGC CTCATCCGTT CCATGCTCAA GGTCAACCCG
    GAGGAGCGGC TGTCCGTCAC CGAGCTGGTC AACCAGCTCC AGGAGATCGC GGCCGCCCGC
    AACGTGAACC CCAAGTCACC CATCACTGAG CTCCTGGAGC AGAATGGAGG CTATGGGCAC
    GCGGCCCCTC CCGGCTCCAC GGGCAGCAGT CTGGTGGTCA CTGAGTACGA GCAGCCCTAT
    GGCGGGTTCC TGGACATTCT GCGTGGGGGC ACCGAGCGCC TCTTCACCAA CATCAAGGAC
    ACCTCCTCCA AAGTCATCCA GTCCGTTGCC AACTATGCGA AGGGAGATCT GGATATTTCT
    TACATCACGT CTAGAATCGC AGTCATGTCG TTCCCGGCGG AGGGCGTGGA GTCGGCCATC
    AAGAACAACA TTGAGGATGT ACGGCTGTTC CTGGACTCGA AGCACCCGGG CCGCTACGCC
    GTCTACAACC TGTCCCCGCG GACCTACCGG CCCTCGAAGT TCCACAACCG GGTCTCCGAC
    TGCGGCTGGG TGGCCCGGCG GGCCCCGAGC CTCCGCAGCC TGTACAGCCT CTGCAGGAAC
    ATGCACGCCT GGCTGCGCCG CGACCCTGGC AACGTCTGCG TGGTGCACTG CATGGACGGG
    AGGGCGGTGT CGGCCGTGGC CGTGTGCTCC TTCTTGTGCT TCTGCCGGCT CTTCAGCACC
    GCGGAGGCCG CTGTGTACAT GTTCAGCATG AAGCGGTGCC CACCGGGCAT CTGGCCATCC
    CACAAGAGGT ACATTGAGTA CATGTGTGAC ATGGTGGCCG AGGAGCCCGT CACGCCCCAC
    AGCAAGCCCA TCCTGGTGAA GGCCGTGGTC ATGACGCCTG TGCCGCTCTT CAGCAAGCAG
    AGGAGCGGCT GCAGGCCCTT CTGCGAGGTC TACGTGGGGG ATGAGCGCGT GACCACCACG
    TCCCAGGAAT ACGACAAGAT GAGAGACTTT AAAATTGAGG ATGGCAAAGC GGTCATTCCC
    CTGGGCATAA CAGTCCAGGG GGACGTGCTC ATCATCATCT ATCATGCGAG GTCCACCCTG
    GGAGGAAGGC TGCAGGCCAA GATGGCGTCC ATGAAGATGT TTCAGATCCA GTTTCACACA
    GGGTTCGTGC CTCGGAACGC AGCTACTGTG AAGTTTGCCA AGTATGATCT GGATGCTTGC
    GACATCCAGG AGAAGTACCC CGACTTGTTC CAAGTGAGCC TGGAGGTGGA GGTGGAGCCC
    AGAGACAGGC CCGGCCACGA GGCCCCGCCC TGGGAGGGTG CAGGCATGCG AGGGCTGAAC
    CCCAAGATCC TCTTCTCCAG CCGGGAGGAG CAGCAAGATG TCCTGTCCAA GTTTGGGAAG
    CCAGAGCTCC CCAGGCAGCC TGGGTCCACC GCACAGTACG ACGCCGAGGC CGGGCCCGCG
    GATGCCGAGC CCCCCGAGTC AGACTCGCCG CACAGCAGCG TGGATGCTGA CCACTTCCTG
    CACACGCTGG ACTGGCGGGA GGATCGGGAC TCCAGTGTGG GCCCGGAGGG TCACCTGGCC
    GAGGAGGGTC CCTCTGCTGT GGCCGAGGAC GGCTGTGTGA GTGAGCCATC GGACGAGGAA
    GCCCCGACAC CCTCTGCGGA GAGCGGGGAC ACAGGCGATG AGGACGTGCC CGCCCCCGCC
    GCCCCGCAGG ACGACAGCGT GGACCTTCTG GGGCTGCACG CGGAGGCGGG GCCTGTGCCC
    GCCCCCCAGG CCCCCGGCGG CCCCCCCAGC AATGCAGACC TGCTCGGCTG CCTGCTCGGG
    GCCCCGGGCC CCGCTCCGGA GGGTGCTGTG GGGGGCCTGC TCGACGGGGA TGCGGCCCTG
    GTCTTCAGCA GCCCAGCCCC CGCGCCTTGC ACCCAGACAA CTGCCCCGGC CGCTGACCCG
    TTCGGCCCGC TCCTGCTGCC GCCCGACGTG GACACCCCTC CTAGCTCGCA GCCTGACCTC
    TTCGGGGAAT TCCTCAACTC AGACGCCCCG GCCGCCCAGC CCGCGCCCTT CCCCTCCACG
    CACAGCGCTC CGCCGCCAGC CTGCAGCACC GACTTCCTGC ATCTTGGGGA CCTGCCCGCC
    GAGCCCAGCA AGATGACAGC CTCTGCCAGC CACCCCGACC TGCTGGGAGC CTGGGATGCC
    TGGGCTGAGG CGCCGGCCCC CGCGCTGGCT GCAGACGGCA CCTTCTTCTC TCCTGGAGAC
    CAGCCAGCCC CTTCTGGCCT TCCGGCTGGC CAGACCAAGT CTCAGAGCCA GGACCCGTTT
    GCAGACCTTG GCGACCTCAG TGCCGGCCTC CAAGGCTCGC CTGCCGCAGC TGCTCCTGGG
    GGCTTTGGTC CCAAAGCAGC GCCCCCTCCC AAGGCCAGCA GCTCCTGGCA GACGAGTCGG
    CCCCCAGCCC AGGGCACCCC GTGGCCCCCC CAGGCCAAGC CGCCCCCCAA AGCCAGTGTA
    CCGCCAAGGC CTAACTACAC CTCCAGCTTC AGTGTGATCG GTAGCCGGGA GGAGCGGGGG
    GTCCGCGCCC CCAGCTTTGG TCCAAAGCCG AGGGTGTCAG AGAACGACTT TGAAGACCTG
    CTCTCCAACC AGGGCTTCTC CTCGCGGGCT GACAGGAAGG GGCCGCGGAC CATCGCTGAG
    ATGAGGAGGC AGGACCAGGC CCGGGACTCG GATCCGCTGA AGCTGAAGCT TCTGGAGTGG
    ACGGAGGGCA AGGAGAGGAA CATCCGCGCC CTGCTGTCCA CGCTGCACAC GGCGCTGTGG
    GATGGCGAGA GCCGCTGGAC GCCGGTGGGC ATGGCCGACC TGGTGACGCC CGCACAAGTG
    AAGAAGCACT ACCGCCGTGC CGTGCTGGTG GTCCACCCCG ACAAGGCCCG GGGGCAGCCC
    TACGAGCAGT ATGCCCGGAT GATCTTCATG GAGCTCAACG ATGCGTGGGC TGAGTTTGAG
    AGCCAGGGCT CCCGGCCACT CTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa20662
    Clone ID Related Accession (Same CDS sequence) XM_010806358.2
    Accession Version XM_010806358.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3543bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product cyclin-G-associated kinase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103986.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010806358.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAAAAAAC TTTCAGGCCA CCCAAACATT GTCCAGTTTT GTTCTGCAGC ATCGATAGGA 
    AAAGAGGAGT CGGACACTGG GCAGGCGGAG TTCCTGCTGC TCATGGAGCT CTGCAGAGGG
    CAGCTGGTGG AATTTTTAAA AAAAGTTGAA TCGAAAGGTC CGCTCTCGTG CGACACCGTC
    CTGAAGATCT TCTACCAGAC GTGCCGGGCG GTGCAGCACA TGCACAGACA GAAGCCGCCC
    ATCATCCACA GGGACCTCAA GGTTGAAAAC TTACTGCTTA GTAACCAGGG GACCATCAAG
    CTGTGCGACT TCGGCAGTGC CACGACCATC TCACACTACC CAGACTACAG CTGGAGCGCC
    CAGCGCCGGG CCCTGGTGGA GGAGGAGATC ACCAGGAACA CGACCCCCAT GTACAGGACG
    CCGGAAATTG TGGACTTGTA CTCAAACTTT CCGATTGGCG AGAAGCAGGA CATCTGGGCC
    CTGGGCTGCA TCCTGTACCT GCTCTGCTTC GGGCAGCACC CCTTTGAGGA CGGAGCGAAG
    CTTCGCATCG TCAACGGGAA GTACGCAATC CCTGCCGACG ACACGCGGTA CTCCGTGTTC
    CACAGCCTCA TCCGTTCCAT GCTCAAGGTC AACCCGGAGG AGCGGCTGTC CGTCACCGAG
    CTGGTCAACC AGCTCCAGGA GATCGCGGCC GCCCGCAACG TGAACCCCAA GTCACCCATC
    ACTGAGCTCC TGGAGCAGAA TGGAGGCTAT GGGCACGCGG CCCCTCCCGG CTCCACGGGC
    AGCAGTCTGG TGGTCACTGA GTACGAGCAG CCCTATGGCG GGTTCCTGGA CATTCTGCGT
    GGGGGCACCG AGCGCCTCTT CACCAACATC AAGGACACCT CCTCCAAAGT CATCCAGTCC
    GTTGCCAACT ATGCGAAGGG AGATCTGGAT ATTTCTTACA TCACGTCTAG AATCGCAGTC
    ATGTCGTTCC CGGCGGAGGG CGTGGAGTCG GCCATCAAGA ACAACATTGA GGATGTACGG
    CTGTTCCTGG ACTCGAAGCA CCCGGGCCGC TACGCCGTCT ACAACCTGTC CCCGCGGACC
    TACCGGCCCT CGAAGTTCCA CAACCGGGTC TCCGACTGCG GCTGGGTGGC CCGGCGGGCC
    CCGAGCCTCC GCAGCCTGTA CAGCCTCTGC AGGAACATGC ACGCCTGGCT GCGCCGCGAC
    CCTGGCAACG TCTGCGTGGT GCACTGCATG GACGGGAGGG CGGTGTCGGC CGTGGCCGTG
    TGCTCCTTCT TGTGCTTCTG CCGGCTCTTC AGCACCGCGG AGGCCGCTGT GTACATGTTC
    AGCATGAAGC GGTGCCCACC GGGCATCTGG CCATCCCACA AGAGGTACAT TGAGTACATG
    TGTGACATGG TGGCCGAGGA GCCCGTCACG CCCCACAGCA AGCCCATCCT GGTGAAGGCC
    GTGGTCATGA CGCCTGTGCC GCTCTTCAGC AAGCAGAGGA GCGGCTGCAG GCCCTTCTGC
    GAGGTCTACG TGGGGGATGA GCGCGTGACC ACCACGTCCC AGGAATACGA CAAGATGAGA
    GACTTTAAAA TTGAGGATGG CAAAGCGGTC ATTCCCCTGG GCATAACAGT CCAGGGGGAC
    GTGCTCATCA TCATCTATCA TGCGAGGTCC ACCCTGGGAG GAAGGCTGCA GGCCAAGATG
    GCGTCCATGA AGATGTTTCA GATCCAGTTT CACACAGGGT TCGTGCCTCG GAACGCAGCT
    ACTGTGAAGT TTGCCAAGTA TGATCTGGAT GCTTGCGACA TCCAGGAGAA GTACCCCGAC
    TTGTTCCAAG TGAGCCTGGA GGTGGAGGTG GAGCCCAGAG ACAGGCCCGG CCACGAGGCC
    CCGCCCTGGG AGGGTGCAGG CATGCGAGGG CTGAACCCCA AGATCCTCTT CTCCAGCCGG
    GAGGAGCAGC AAGATGTCCT GTCCAAGTTT GGGAAGCCAG AGCTCCCCAG GCAGCCTGGG
    TCCACCGCAC AGTACGACGC CGAGGCCGGG CCCGCGGATG CCGAGCCCCC CGAGTCAGAC
    TCGCCGCACA GCAGCGTGGA TGCTGACCAC TTCCTGCACA CGCTGGACTG GCGGGAGGAT
    CGGGACTCCA GTGTGGGCCC GGAGGGTCAC CTGGCCGAGG AGGGTCCCTC TGCTGTGGCC
    GAGGACGGCT GTGTGAGTGA GCCATCGGAC GAGGAAGCCC CGACACCCTC TGCGGAGAGC
    GGGGACACAG GCGATGAGGA CGTGCCCGCC CCCGCCGCCC CGCAGGACGA CAGCGTGGAC
    CTTCTGGGGC TGCACGCGGA GGCGGGGCCT GTGCCCGCCC CCCAGGCCCC CGGCGGCCCC
    CCCAGCAATG CAGACCTGCT CGGCTGCCTG CTCGGGGCCC CGGGCCCCGC TCCGGAGGGT
    GCTGTGGGGG GCCTGCTCGA CGGGGATGCG GCCCTGCTCT TCAGCAGCCC AGCCCCCGCG
    CCTTGCACCC AGACAACTGC CCCGGCCGCT GACCCGTTCG GCCCGCTCCT GCTGCCGCCC
    GACGTGGACA CCCCTCCTAG CTCGCAGCCT GACCTCTTCG GGGAATTCCT CAACTCAGAC
    GCCCCGGCCG CCCAGCCCGC GCCCTTCCCC TCCACCCACA GCGCTCCGCC GCCAGCCTGC
    AGCACCGACT TCCTGCATCT TGGGGACCTG CCCGCCGAGC CCAGCAAGAT GACAGCCTCT
    GCCAGCCACC CCGACCTGCT GGGAGCCTGG GATGCCTGGG CTGAGGCGCC GGCCCCCGCG
    CTGGCTGCAG ACGGCACCTT CTTCTCTCCT GGAGACCAGC CAGCCCCTTC TGGCCTTCCG
    GCTGGCCAGA CCAAGTCTCA GAGCCAGGAC CCGTTTGCAG ACCTTGGCGA CCTCAGTGCC
    GGCCTCCAAG GCTCGCCTGC CGCAGCTGCT CCTGGGGGCT TTGGTCCCAA AGCAGCGCCC
    CCTCCCAAGG CCAGCAGCTC CTGGCAGACG AGTCGGCCCC CAGCCCAGGG CACCCCGTGG
    CCCCCCCAGG CCAAGCCGCC CCCCAAAGCC AGTGTACCGC CAAGGCCTAA CTACACCTCC
    AGCTTCAGTG TGATCGGTAG CCGGGAGGAG CGGGGGGTCC GCGCCCCCAG CTTTGGTCCA
    AAGCCGAGGG TGTCAGAAAA CGACTTTGAA GACCTGCTCT CCAACCAGGG CTTCTCCTCG
    CGGGCTGACA GGAAGGGGCC GCGGACCATC GCTGAGATGA GGAGGCAGGA CCAGGCCCGG
    GACTTGGATC CGCTGAAGCT GAAGCTTCTG GAGTGGACGG AGGGCAAGGA GAGGAACATC
    CGCGCCCTGC TGTCCACGCT GCACACGGCG CTGTGGGACG GCGAGAGCCG CTGGACGCCG
    GTGGGCATGG CCGACCTGGT GACGCCCGCA CAAGTGAAGA AGCACTACCG CCGTGCCGTG
    CTGGTGGTCC ACCCCGACAA GGCCCGGGGG CAGCCCTACG AGCAGTATGC CCGGATGATC
    TTCATGGAGC TCAACGATGC GTGGGCTGAG TTTGAGAGCC AGGGCTCCCG GCCACTCTTC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010804660.1
    CDS290..3832
    Translation

    Target ORF information:

    RefSeq Version XM_010806358.2
    Organism Bos taurus(cattle)
    Definition Bos taurus cyclin G associated kinase (GAK), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806358.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAAAAAAC TTTCAGGCCA CCCAAACATT GTCCAGTTTT GTTCTGCAGC ATCGATAGGA 
    AAAGAGGAGT CGGACACTGG GCAGGCGGAG TTCCTGCTGC TCATGGAGCT CTGCAGAGGG
    CAGCTGGTGG AATTTTTAAA AAAAGTTGAA TCGAAAGGTC CGCTCTCGTG CGACACCGTC
    CTGAAGATCT TCTACCAGAC GTGCCGGGCG GTGCAGCACA TGCACAGACA GAAGCCGCCC
    ATCATCCACA GGGACCTCAA GGTTGAAAAC TTACTGCTTA GTAACCAGGG GACCATCAAG
    CTGTGCGACT TCGGCAGTGC CACGACCATC TCACACTACC CAGACTACAG CTGGAGCGCC
    CAGCGCCGGG CCCTGGTGGA GGAGGAGATC ACCAGGAACA CGACCCCCAT GTACAGGACG
    CCGGAAATTG TGGACTTGTA CTCAAACTTT CCGATTGGCG AGAAGCAGGA CATCTGGGCC
    CTGGGCTGCA TCCTGTACCT GCTCTGCTTC GGGCAGCACC CCTTTGAGGA CGGAGCGAAG
    CTTCGCATCG TCAACGGGAA GTACGCAATC CCTGCCGACG ACACGCGGTA CTCCGTGTTC
    CACAGCCTCA TCCGTTCCAT GCTCAAGGTC AACCCGGAGG AGCGGCTGTC CGTCACCGAG
    CTGGTCAACC AGCTCCAGGA GATCGCGGCC GCCCGCAACG TGAACCCCAA GTCACCCATC
    ACTGAGCTCC TGGAGCAGAA TGGAGGCTAT GGGCACGCGG CCCCTCCCGG CTCCACGGGC
    AGCAGTCTGG TGGTCACTGA GTACGAGCAG CCCTATGGCG GGTTCCTGGA CATTCTGCGT
    GGGGGCACCG AGCGCCTCTT CACCAACATC AAGGACACCT CCTCCAAAGT CATCCAGTCC
    GTTGCCAACT ATGCGAAGGG AGATCTGGAT ATTTCTTACA TCACGTCTAG AATCGCAGTC
    ATGTCGTTCC CGGCGGAGGG CGTGGAGTCG GCCATCAAGA ACAACATTGA GGATGTACGG
    CTGTTCCTGG ACTCGAAGCA CCCGGGCCGC TACGCCGTCT ACAACCTGTC CCCGCGGACC
    TACCGGCCCT CGAAGTTCCA CAACCGGGTC TCCGACTGCG GCTGGGTGGC CCGGCGGGCC
    CCGAGCCTCC GCAGCCTGTA CAGCCTCTGC AGGAACATGC ACGCCTGGCT GCGCCGCGAC
    CCTGGCAACG TCTGCGTGGT GCACTGCATG GACGGGAGGG CGGTGTCGGC CGTGGCCGTG
    TGCTCCTTCT TGTGCTTCTG CCGGCTCTTC AGCACCGCGG AGGCCGCTGT GTACATGTTC
    AGCATGAAGC GGTGCCCACC GGGCATCTGG CCATCCCACA AGAGGTACAT TGAGTACATG
    TGTGACATGG TGGCCGAGGA GCCCGTCACG CCCCACAGCA AGCCCATCCT GGTGAAGGCC
    GTGGTCATGA CGCCTGTGCC GCTCTTCAGC AAGCAGAGGA GCGGCTGCAG GCCCTTCTGC
    GAGGTCTACG TGGGGGATGA GCGCGTGACC ACCACGTCCC AGGAATACGA CAAGATGAGA
    GACTTTAAAA TTGAGGATGG CAAAGCGGTC ATTCCCCTGG GCATAACAGT CCAGGGGGAC
    GTGCTCATCA TCATCTATCA TGCGAGGTCC ACCCTGGGAG GAAGGCTGCA GGCCAAGATG
    GCGTCCATGA AGATGTTTCA GATCCAGTTT CACACAGGGT TCGTGCCTCG GAACGCAGCT
    ACTGTGAAGT TTGCCAAGTA TGATCTGGAT GCTTGCGACA TCCAGGAGAA GTACCCCGAC
    TTGTTCCAAG TGAGCCTGGA GGTGGAGGTG GAGCCCAGAG ACAGGCCCGG CCACGAGGCC
    CCGCCCTGGG AGGGTGCAGG CATGCGAGGG CTGAACCCCA AGATCCTCTT CTCCAGCCGG
    GAGGAGCAGC AAGATGTCCT GTCCAAGTTT GGGAAGCCAG AGCTCCCCAG GCAGCCTGGG
    TCCACCGCAC AGTACGACGC CGAGGCCGGG CCCGCGGATG CCGAGCCCCC CGAGTCAGAC
    TCGCCGCACA GCAGCGTGGA TGCTGACCAC TTCCTGCACA CGCTGGACTG GCGGGAGGAT
    CGGGACTCCA GTGTGGGCCC GGAGGGTCAC CTGGCCGAGG AGGGTCCCTC TGCTGTGGCC
    GAGGACGGCT GTGTGAGTGA GCCATCGGAC GAGGAAGCCC CGACACCCTC TGCGGAGAGC
    GGGGACACAG GCGATGAGGA CGTGCCCGCC CCCGCCGCCC CGCAGGACGA CAGCGTGGAC
    CTTCTGGGGC TGCACGCGGA GGCGGGGCCT GTGCCCGCCC CCCAGGCCCC CGGCGGCCCC
    CCCAGCAATG CAGACCTGCT CGGCTGCCTG CTCGGGGCCC CGGGCCCCGC TCCGGAGGGT
    GCTGTGGGGG GCCTGCTCGA CGGGGATGCG GCCCTGCTCT TCAGCAGCCC AGCCCCCGCG
    CCTTGCACCC AGACAACTGC CCCGGCCGCT GACCCGTTCG GCCCGCTCCT GCTGCCGCCC
    GACGTGGACA CCCCTCCTAG CTCGCAGCCT GACCTCTTCG GGGAATTCCT CAACTCAGAC
    GCCCCGGCCG CCCAGCCCGC GCCCTTCCCC TCCACCCACA GCGCTCCGCC GCCAGCCTGC
    AGCACCGACT TCCTGCATCT TGGGGACCTG CCCGCCGAGC CCAGCAAGAT GACAGCCTCT
    GCCAGCCACC CCGACCTGCT GGGAGCCTGG GATGCCTGGG CTGAGGCGCC GGCCCCCGCG
    CTGGCTGCAG ACGGCACCTT CTTCTCTCCT GGAGACCAGC CAGCCCCTTC TGGCCTTCCG
    GCTGGCCAGA CCAAGTCTCA GAGCCAGGAC CCGTTTGCAG ACCTTGGCGA CCTCAGTGCC
    GGCCTCCAAG GCTCGCCTGC CGCAGCTGCT CCTGGGGGCT TTGGTCCCAA AGCAGCGCCC
    CCTCCCAAGG CCAGCAGCTC CTGGCAGACG AGTCGGCCCC CAGCCCAGGG CACCCCGTGG
    CCCCCCCAGG CCAAGCCGCC CCCCAAAGCC AGTGTACCGC CAAGGCCTAA CTACACCTCC
    AGCTTCAGTG TGATCGGTAG CCGGGAGGAG CGGGGGGTCC GCGCCCCCAG CTTTGGTCCA
    AAGCCGAGGG TGTCAGAAAA CGACTTTGAA GACCTGCTCT CCAACCAGGG CTTCTCCTCG
    CGGGCTGACA GGAAGGGGCC GCGGACCATC GCTGAGATGA GGAGGCAGGA CCAGGCCCGG
    GACTTGGATC CGCTGAAGCT GAAGCTTCTG GAGTGGACGG AGGGCAAGGA GAGGAACATC
    CGCGCCCTGC TGTCCACGCT GCACACGGCG CTGTGGGACG GCGAGAGCCG CTGGACGCCG
    GTGGGCATGG CCGACCTGGT GACGCCCGCA CAAGTGAAGA AGCACTACCG CCGTGCCGTG
    CTGGTGGTCC ACCCCGACAA GGCCCGGGGG CAGCCCTACG AGCAGTATGC CCGGATGATC
    TTCATGGAGC TCAACGATGC GTGGGCTGAG TTTGAGAGCC AGGGCTCCCG GCCACTCTTC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa20663
    Clone ID Related Accession (Same CDS sequence) XM_015471751.1 , XM_005208283.1
    Accession Version XM_015471751.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3441bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product cyclin-G-associated kinase isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103986.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGAGCTCT GCAGAGGGCA GCTGGTGGAA TTTTTAAAAA AAGTTGAATC GAAAGGTCCG 
    CTCTCGTGCG ACACCGTCCT GAAGATCTTC TACCAGACGT GCCGGGCGGT GCAGCACATG
    CACAGACAGA AGCCGCCCAT CATCCACAGG GACCTCAAGG TTGAAAACTT ACTGCTTAGT
    AACCAGGGGA CCATCAAGCT GTGCGACTTC GGCAGTGCCA CGACCATCTC ACACTACCCA
    GACTACAGCT GGAGCGCCCA GCGCCGGGCC CTGGTGGAGG AGGAGATCAC CAGGAACACG
    ACCCCCATGT ACAGGACGCC GGAAATTGTG GACTTGTACT CAAACTTTCC GATTGGCGAG
    AAGCAGGACA TCTGGGCCCT GGGCTGCATC CTGTACCTGC TCTGCTTCGG GCAGCACCCC
    TTTGAGGACG GAGCGAAGCT TCGCATCGTC AACGGGAAGT ACGCAATCCC TGCCGACGAC
    ACGCGGTACT CCGTGTTCCA CAGCCTCATC CGTTCCATGC TCAAGGTCAA CCCGGAGGAG
    CGGCTGTCCG TCACCGAGCT GGTCAACCAG CTCCAGGAGA TCGCGGCCGC CCGCAACGTG
    AACCCCAAGT CACCCATCAC TGAGCTCCTG GAGCAGAATG GAGGCTATGG GCACGCGGCC
    CCTCCCGGCT CCACGGGCAG CAGTCTGGTG GTCACTGAGT ACGAGCAGCC CTATGGCGGG
    TTCCTGGACA TTCTGCGTGG GGGCACCGAG CGCCTCTTCA CCAACATCAA GGACACCTCC
    TCCAAAGTCA TCCAGTCCGT TGCCAACTAT GCGAAGGGAG ATCTGGATAT TTCTTACATC
    ACGTCTAGAA TCGCAGTCAT GTCGTTCCCG GCGGAGGGCG TGGAGTCGGC CATCAAGAAC
    AACATTGAGG ATGTACGGCT GTTCCTGGAC TCGAAGCACC CGGGCCGCTA CGCCGTCTAC
    AACCTGTCCC CGCGGACCTA CCGGCCCTCG AAGTTCCACA ACCGGGTCTC CGACTGCGGC
    TGGGTGGCCC GGCGGGCCCC GAGCCTCCGC AGCCTGTACA GCCTCTGCAG GAACATGCAC
    GCCTGGCTGC GCCGCGACCC TGGCAACGTC TGCGTGGTGC ACTGCATGGA CGGGAGGGCG
    GTGTCGGCCG TGGCCGTGTG CTCCTTCTTG TGCTTCTGCC GGCTCTTCAG CACCGCGGAG
    GCCGCTGTGT ACATGTTCAG CATGAAGCGG TGCCCACCGG GCATCTGGCC ATCCCACAAG
    AGGTACATTG AGTACATGTG TGACATGGTG GCCGAGGAGC CCGTCACGCC CCACAGCAAG
    CCCATCCTGG TGAAGGCCGT GGTCATGACG CCTGTGCCGC TCTTCAGCAA GCAGAGGAGC
    GGCTGCAGGC CCTTCTGCGA GGTCTACGTG GGGGATGAGC GCGTGACCAC CACGTCCCAG
    GAATACGACA AGATGAGAGA CTTTAAAATT GAGGATGGCA AAGCGGTCAT TCCCCTGGGC
    ATAACAGTCC AGGGGGACGT GCTCATCATC ATCTATCATG CGAGGTCCAC CCTGGGAGGA
    AGGCTGCAGG CCAAGATGGC GTCCATGAAG ATGTTTCAGA TCCAGTTTCA CACAGGGTTC
    GTGCCTCGGA ACGCAGCTAC TGTGAAGTTT GCCAAGTATG ATCTGGATGC TTGCGACATC
    CAGGAGAAGT ACCCCGACTT GTTCCAAGTG AGCCTGGAGG TGGAGGTGGA GCCCAGAGAC
    AGGCCCGGCC ACGAGGCCCC GCCCTGGGAG GGTGCAGGCA TGCGAGGGCT GAACCCCAAG
    ATCCTCTTCT CCAGCCGGGA GGAGCAGCAA GATGTCCTGT CCAAGTTTGG GAAGCCAGAG
    CTCCCCAGGC AGCCTGGGTC CACCGCACAG TACGACGCCG AGGCCGGGCC CGCGGATGCC
    GAGCCCCCCG AGTCAGACTC GCCGCACAGC AGCGTGGATG CTGACCACTT CCTGCACACG
    CTGGACTGGC GGGAGGATCG GGACTCCAGT GTGGGCCCGG AGGGTCACCT GGCCGAGGAG
    GGTCCCTCTG CTGTGGCCGA GGACGGCTGT GTGAGTGAGC CATCGGACGA GGAAGCCCCG
    ACACCCTCTG CGGAGAGCGG GGACACAGGC GATGAGGACG TGCCCGCCCC CGCCGCCCCG
    CAGGACGACA GCGTGGACCT TCTGGGGCTG CACGCGGAGG CGGGGCCTGT GCCCGCCCCC
    CAGGCCCCCG GCGGCCCCCC CAGCAATGCA GACCTGCTCG GCTGCCTGCT CGGGGCCCCG
    GGCCCCGCTC CGGAGGGTGC TGTGGGGGGC CTGCTCGACG GGGATGCGGC CCTGCTCTTC
    AGCAGCCCAG CCCCCGCGCC TTGCACCCAG ACAACTGCCC CGGCCGCTGA CCCGTTCGGC
    CCGCTCCTGC TGCCGCCCGA CGTGGACACC CCTCCTAGCT CGCAGCCTGA CCTCTTCGGG
    GAATTCCTCA ACTCAGACGC CCCGGCCGCC CAGCCCGCGC CCTTCCCCTC CACCCACAGC
    GCTCCGCCGC CAGCCTGCAG CACCGACTTC CTGCATCTTG GGGACCTGCC CGCCGAGCCC
    AGCAAGATGA CAGCCTCTGC CAGCCACCCC GACCTGCTGG GAGCCTGGGA TGCCTGGGCT
    GAGGCGCCGG CCCCCGCGCT GGCTGCAGAC GGCACCTTCT TCTCTCCTGG AGACCAGCCA
    GCCCCTTCTG GCCTTCCGGC TGGCCAGACC AAGTCTCAGA GCCAGGACCC GTTTGCAGAC
    CTTGGCGACC TCAGTGCCGG CCTCCAAGGC TCGCCTGCCG CAGCTGCTCC TGGGGGCTTT
    GGTCCCAAAG CAGCGCCCCC TCCCAAGGCC AGCAGCTCCT GGCAGACGAG TCGGCCCCCA
    GCCCAGGGCA CCCCGTGGCC CCCCCAGGCC AAGCCGCCCC CCAAAGCCAG TGTACCGCCA
    AGGCCTAACT ACACCTCCAG CTTCAGTGTG ATCGGTAGCC GGGAGGAGCG GGGGGTCCGC
    GCCCCCAGCT TTGGTCCAAA GCCGAGGGTG TCAGAAAACG ACTTTGAAGA CCTGCTCTCC
    AACCAGGGCT TCTCCTCGCG GGCTGACAGG AAGGGGCCGC GGACCATCGC TGAGATGAGG
    AGGCAGGACC AGGCCCGGGA CTTGGATCCG CTGAAGCTGA AGCTTCTGGA GTGGACGGAG
    GGCAAGGAGA GGAACATCCG CGCCCTGCTG TCCACGCTGC ACACGGCGCT GTGGGACGGC
    GAGAGCCGCT GGACGCCGGT GGGCATGGCC GACCTGGTGA CGCCCGCACA AGTGAAGAAG
    CACTACCGCC GTGCCGTGCT GGTGGTCCAC CCCGACAAGG CCCGGGGGCA GCCCTACGAG
    CAGTATGCCC GGATGATCTT CATGGAGCTC AACGATGCGT GGGCTGAGTT TGAGAGCCAG
    GGCTCCCGGC CACTCTTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015327237.1
    CDS340..3780
    Translation

    Target ORF information:

    RefSeq Version XM_015471751.1
    Organism Bos taurus(cattle)
    Definition Bos taurus cyclin G associated kinase (GAK), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471751.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGAGCTCT GCAGAGGGCA GCTGGTGGAA TTTTTAAAAA AAGTTGAATC GAAAGGTCCG 
    CTCTCGTGCG ACACCGTCCT GAAGATCTTC TACCAGACGT GCCGGGCGGT GCAGCACATG
    CACAGACAGA AGCCGCCCAT CATCCACAGG GACCTCAAGG TTGAAAACTT ACTGCTTAGT
    AACCAGGGGA CCATCAAGCT GTGCGACTTC GGCAGTGCCA CGACCATCTC ACACTACCCA
    GACTACAGCT GGAGCGCCCA GCGCCGGGCC CTGGTGGAGG AGGAGATCAC CAGGAACACG
    ACCCCCATGT ACAGGACGCC GGAAATTGTG GACTTGTACT CAAACTTTCC GATTGGCGAG
    AAGCAGGACA TCTGGGCCCT GGGCTGCATC CTGTACCTGC TCTGCTTCGG GCAGCACCCC
    TTTGAGGACG GAGCGAAGCT TCGCATCGTC AACGGGAAGT ACGCAATCCC TGCCGACGAC
    ACGCGGTACT CCGTGTTCCA CAGCCTCATC CGTTCCATGC TCAAGGTCAA CCCGGAGGAG
    CGGCTGTCCG TCACCGAGCT GGTCAACCAG CTCCAGGAGA TCGCGGCCGC CCGCAACGTG
    AACCCCAAGT CACCCATCAC TGAGCTCCTG GAGCAGAATG GAGGCTATGG GCACGCGGCC
    CCTCCCGGCT CCACGGGCAG CAGTCTGGTG GTCACTGAGT ACGAGCAGCC CTATGGCGGG
    TTCCTGGACA TTCTGCGTGG GGGCACCGAG CGCCTCTTCA CCAACATCAA GGACACCTCC
    TCCAAAGTCA TCCAGTCCGT TGCCAACTAT GCGAAGGGAG ATCTGGATAT TTCTTACATC
    ACGTCTAGAA TCGCAGTCAT GTCGTTCCCG GCGGAGGGCG TGGAGTCGGC CATCAAGAAC
    AACATTGAGG ATGTACGGCT GTTCCTGGAC TCGAAGCACC CGGGCCGCTA CGCCGTCTAC
    AACCTGTCCC CGCGGACCTA CCGGCCCTCG AAGTTCCACA ACCGGGTCTC CGACTGCGGC
    TGGGTGGCCC GGCGGGCCCC GAGCCTCCGC AGCCTGTACA GCCTCTGCAG GAACATGCAC
    GCCTGGCTGC GCCGCGACCC TGGCAACGTC TGCGTGGTGC ACTGCATGGA CGGGAGGGCG
    GTGTCGGCCG TGGCCGTGTG CTCCTTCTTG TGCTTCTGCC GGCTCTTCAG CACCGCGGAG
    GCCGCTGTGT ACATGTTCAG CATGAAGCGG TGCCCACCGG GCATCTGGCC ATCCCACAAG
    AGGTACATTG AGTACATGTG TGACATGGTG GCCGAGGAGC CCGTCACGCC CCACAGCAAG
    CCCATCCTGG TGAAGGCCGT GGTCATGACG CCTGTGCCGC TCTTCAGCAA GCAGAGGAGC
    GGCTGCAGGC CCTTCTGCGA GGTCTACGTG GGGGATGAGC GCGTGACCAC CACGTCCCAG
    GAATACGACA AGATGAGAGA CTTTAAAATT GAGGATGGCA AAGCGGTCAT TCCCCTGGGC
    ATAACAGTCC AGGGGGACGT GCTCATCATC ATCTATCATG CGAGGTCCAC CCTGGGAGGA
    AGGCTGCAGG CCAAGATGGC GTCCATGAAG ATGTTTCAGA TCCAGTTTCA CACAGGGTTC
    GTGCCTCGGA ACGCAGCTAC TGTGAAGTTT GCCAAGTATG ATCTGGATGC TTGCGACATC
    CAGGAGAAGT ACCCCGACTT GTTCCAAGTG AGCCTGGAGG TGGAGGTGGA GCCCAGAGAC
    AGGCCCGGCC ACGAGGCCCC GCCCTGGGAG GGTGCAGGCA TGCGAGGGCT GAACCCCAAG
    ATCCTCTTCT CCAGCCGGGA GGAGCAGCAA GATGTCCTGT CCAAGTTTGG GAAGCCAGAG
    CTCCCCAGGC AGCCTGGGTC CACCGCACAG TACGACGCCG AGGCCGGGCC CGCGGATGCC
    GAGCCCCCCG AGTCAGACTC GCCGCACAGC AGCGTGGATG CTGACCACTT CCTGCACACG
    CTGGACTGGC GGGAGGATCG GGACTCCAGT GTGGGCCCGG AGGGTCACCT GGCCGAGGAG
    GGTCCCTCTG CTGTGGCCGA GGACGGCTGT GTGAGTGAGC CATCGGACGA GGAAGCCCCG
    ACACCCTCTG CGGAGAGCGG GGACACAGGC GATGAGGACG TGCCCGCCCC CGCCGCCCCG
    CAGGACGACA GCGTGGACCT TCTGGGGCTG CACGCGGAGG CGGGGCCTGT GCCCGCCCCC
    CAGGCCCCCG GCGGCCCCCC CAGCAATGCA GACCTGCTCG GCTGCCTGCT CGGGGCCCCG
    GGCCCCGCTC CGGAGGGTGC TGTGGGGGGC CTGCTCGACG GGGATGCGGC CCTGCTCTTC
    AGCAGCCCAG CCCCCGCGCC TTGCACCCAG ACAACTGCCC CGGCCGCTGA CCCGTTCGGC
    CCGCTCCTGC TGCCGCCCGA CGTGGACACC CCTCCTAGCT CGCAGCCTGA CCTCTTCGGG
    GAATTCCTCA ACTCAGACGC CCCGGCCGCC CAGCCCGCGC CCTTCCCCTC CACCCACAGC
    GCTCCGCCGC CAGCCTGCAG CACCGACTTC CTGCATCTTG GGGACCTGCC CGCCGAGCCC
    AGCAAGATGA CAGCCTCTGC CAGCCACCCC GACCTGCTGG GAGCCTGGGA TGCCTGGGCT
    GAGGCGCCGG CCCCCGCGCT GGCTGCAGAC GGCACCTTCT TCTCTCCTGG AGACCAGCCA
    GCCCCTTCTG GCCTTCCGGC TGGCCAGACC AAGTCTCAGA GCCAGGACCC GTTTGCAGAC
    CTTGGCGACC TCAGTGCCGG CCTCCAAGGC TCGCCTGCCG CAGCTGCTCC TGGGGGCTTT
    GGTCCCAAAG CAGCGCCCCC TCCCAAGGCC AGCAGCTCCT GGCAGACGAG TCGGCCCCCA
    GCCCAGGGCA CCCCGTGGCC CCCCCAGGCC AAGCCGCCCC CCAAAGCCAG TGTACCGCCA
    AGGCCTAACT ACACCTCCAG CTTCAGTGTG ATCGGTAGCC GGGAGGAGCG GGGGGTCCGC
    GCCCCCAGCT TTGGTCCAAA GCCGAGGGTG TCAGAAAACG ACTTTGAAGA CCTGCTCTCC
    AACCAGGGCT TCTCCTCGCG GGCTGACAGG AAGGGGCCGC GGACCATCGC TGAGATGAGG
    AGGCAGGACC AGGCCCGGGA CTTGGATCCG CTGAAGCTGA AGCTTCTGGA GTGGACGGAG
    GGCAAGGAGA GGAACATCCG CGCCCTGCTG TCCACGCTGC ACACGGCGCT GTGGGACGGC
    GAGAGCCGCT GGACGCCGGT GGGCATGGCC GACCTGGTGA CGCCCGCACA AGTGAAGAAG
    CACTACCGCC GTGCCGTGCT GGTGGTCCAC CCCGACAAGG CCCGGGGGCA GCCCTACGAG
    CAGTATGCCC GGATGATCTT CATGGAGCTC AACGATGCGT GGGCTGAGTT TGAGAGCCAG
    GGCTCCCGGC CACTCTTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa20663
    Clone ID Related Accession (Same CDS sequence) XM_015471751.1 , XM_005208283.1
    Accession Version XM_005208283.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3441bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product cyclin-G-associated kinase isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103986.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGAGCTCT GCAGAGGGCA GCTGGTGGAA TTTTTAAAAA AAGTTGAATC GAAAGGTCCG 
    CTCTCGTGCG ACACCGTCCT GAAGATCTTC TACCAGACGT GCCGGGCGGT GCAGCACATG
    CACAGACAGA AGCCGCCCAT CATCCACAGG GACCTCAAGG TTGAAAACTT ACTGCTTAGT
    AACCAGGGGA CCATCAAGCT GTGCGACTTC GGCAGTGCCA CGACCATCTC ACACTACCCA
    GACTACAGCT GGAGCGCCCA GCGCCGGGCC CTGGTGGAGG AGGAGATCAC CAGGAACACG
    ACCCCCATGT ACAGGACGCC GGAAATTGTG GACTTGTACT CAAACTTTCC GATTGGCGAG
    AAGCAGGACA TCTGGGCCCT GGGCTGCATC CTGTACCTGC TCTGCTTCGG GCAGCACCCC
    TTTGAGGACG GAGCGAAGCT TCGCATCGTC AACGGGAAGT ACGCAATCCC TGCCGACGAC
    ACGCGGTACT CCGTGTTCCA CAGCCTCATC CGTTCCATGC TCAAGGTCAA CCCGGAGGAG
    CGGCTGTCCG TCACCGAGCT GGTCAACCAG CTCCAGGAGA TCGCGGCCGC CCGCAACGTG
    AACCCCAAGT CACCCATCAC TGAGCTCCTG GAGCAGAATG GAGGCTATGG GCACGCGGCC
    CCTCCCGGCT CCACGGGCAG CAGTCTGGTG GTCACTGAGT ACGAGCAGCC CTATGGCGGG
    TTCCTGGACA TTCTGCGTGG GGGCACCGAG CGCCTCTTCA CCAACATCAA GGACACCTCC
    TCCAAAGTCA TCCAGTCCGT TGCCAACTAT GCGAAGGGAG ATCTGGATAT TTCTTACATC
    ACGTCTAGAA TCGCAGTCAT GTCGTTCCCG GCGGAGGGCG TGGAGTCGGC CATCAAGAAC
    AACATTGAGG ATGTACGGCT GTTCCTGGAC TCGAAGCACC CGGGCCGCTA CGCCGTCTAC
    AACCTGTCCC CGCGGACCTA CCGGCCCTCG AAGTTCCACA ACCGGGTCTC CGACTGCGGC
    TGGGTGGCCC GGCGGGCCCC GAGCCTCCGC AGCCTGTACA GCCTCTGCAG GAACATGCAC
    GCCTGGCTGC GCCGCGACCC TGGCAACGTC TGCGTGGTGC ACTGCATGGA CGGGAGGGCG
    GTGTCGGCCG TGGCCGTGTG CTCCTTCTTG TGCTTCTGCC GGCTCTTCAG CACCGCGGAG
    GCCGCTGTGT ACATGTTCAG CATGAAGCGG TGCCCACCGG GCATCTGGCC ATCCCACAAG
    AGGTACATTG AGTACATGTG TGACATGGTG GCCGAGGAGC CCGTCACGCC CCACAGCAAG
    CCCATCCTGG TGAAGGCCGT GGTCATGACG CCTGTGCCGC TCTTCAGCAA GCAGAGGAGC
    GGCTGCAGGC CCTTCTGCGA GGTCTACGTG GGGGATGAGC GCGTGACCAC CACGTCCCAG
    GAATACGACA AGATGAGAGA CTTTAAAATT GAGGATGGCA AAGCGGTCAT TCCCCTGGGC
    ATAACAGTCC AGGGGGACGT GCTCATCATC ATCTATCATG CGAGGTCCAC CCTGGGAGGA
    AGGCTGCAGG CCAAGATGGC GTCCATGAAG ATGTTTCAGA TCCAGTTTCA CACAGGGTTC
    GTGCCTCGGA ACGCAGCTAC TGTGAAGTTT GCCAAGTATG ATCTGGATGC TTGCGACATC
    CAGGAGAAGT ACCCCGACTT GTTCCAAGTG AGCCTGGAGG TGGAGGTGGA GCCCAGAGAC
    AGGCCCGGCC ACGAGGCCCC GCCCTGGGAG GGTGCAGGCA TGCGAGGGCT GAACCCCAAG
    ATCCTCTTCT CCAGCCGGGA GGAGCAGCAA GATGTCCTGT CCAAGTTTGG GAAGCCAGAG
    CTCCCCAGGC AGCCTGGGTC CACCGCACAG TACGACGCCG AGGCCGGGCC CGCGGATGCC
    GAGCCCCCCG AGTCAGACTC GCCGCACAGC AGCGTGGATG CTGACCACTT CCTGCACACG
    CTGGACTGGC GGGAGGATCG GGACTCCAGT GTGGGCCCGG AGGGTCACCT GGCCGAGGAG
    GGTCCCTCTG CTGTGGCCGA GGACGGCTGT GTGAGTGAGC CATCGGACGA GGAAGCCCCG
    ACACCCTCTG CGGAGAGCGG GGACACAGGC GATGAGGACG TGCCCGCCCC CGCCGCCCCG
    CAGGACGACA GCGTGGACCT TCTGGGGCTG CACGCGGAGG CGGGGCCTGT GCCCGCCCCC
    CAGGCCCCCG GCGGCCCCCC CAGCAATGCA GACCTGCTCG GCTGCCTGCT CGGGGCCCCG
    GGCCCCGCTC CGGAGGGTGC TGTGGGGGGC CTGCTCGACG GGGATGCGGC CCTGCTCTTC
    AGCAGCCCAG CCCCCGCGCC TTGCACCCAG ACAACTGCCC CGGCCGCTGA CCCGTTCGGC
    CCGCTCCTGC TGCCGCCCGA CGTGGACACC CCTCCTAGCT CGCAGCCTGA CCTCTTCGGG
    GAATTCCTCA ACTCAGACGC CCCGGCCGCC CAGCCCGCGC CCTTCCCCTC CACCCACAGC
    GCTCCGCCGC CAGCCTGCAG CACCGACTTC CTGCATCTTG GGGACCTGCC CGCCGAGCCC
    AGCAAGATGA CAGCCTCTGC CAGCCACCCC GACCTGCTGG GAGCCTGGGA TGCCTGGGCT
    GAGGCGCCGG CCCCCGCGCT GGCTGCAGAC GGCACCTTCT TCTCTCCTGG AGACCAGCCA
    GCCCCTTCTG GCCTTCCGGC TGGCCAGACC AAGTCTCAGA GCCAGGACCC GTTTGCAGAC
    CTTGGCGACC TCAGTGCCGG CCTCCAAGGC TCGCCTGCCG CAGCTGCTCC TGGGGGCTTT
    GGTCCCAAAG CAGCGCCCCC TCCCAAGGCC AGCAGCTCCT GGCAGACGAG TCGGCCCCCA
    GCCCAGGGCA CCCCGTGGCC CCCCCAGGCC AAGCCGCCCC CCAAAGCCAG TGTACCGCCA
    AGGCCTAACT ACACCTCCAG CTTCAGTGTG ATCGGTAGCC GGGAGGAGCG GGGGGTCCGC
    GCCCCCAGCT TTGGTCCAAA GCCGAGGGTG TCAGAAAACG ACTTTGAAGA CCTGCTCTCC
    AACCAGGGCT TCTCCTCGCG GGCTGACAGG AAGGGGCCGC GGACCATCGC TGAGATGAGG
    AGGCAGGACC AGGCCCGGGA CTTGGATCCG CTGAAGCTGA AGCTTCTGGA GTGGACGGAG
    GGCAAGGAGA GGAACATCCG CGCCCTGCTG TCCACGCTGC ACACGGCGCT GTGGGACGGC
    GAGAGCCGCT GGACGCCGGT GGGCATGGCC GACCTGGTGA CGCCCGCACA AGTGAAGAAG
    CACTACCGCC GTGCCGTGCT GGTGGTCCAC CCCGACAAGG CCCGGGGGCA GCCCTACGAG
    CAGTATGCCC GGATGATCTT CATGGAGCTC AACGATGCGT GGGCTGAGTT TGAGAGCCAG
    GGCTCCCGGC CACTCTTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005208340.1
    CDS663..4103
    Translation

    Target ORF information:

    RefSeq Version XM_005208283.1
    Organism Bos taurus(cattle)
    Definition Bos taurus cyclin G associated kinase (GAK), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005208283.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGAGCTCT GCAGAGGGCA GCTGGTGGAA TTTTTAAAAA AAGTTGAATC GAAAGGTCCG 
    CTCTCGTGCG ACACCGTCCT GAAGATCTTC TACCAGACGT GCCGGGCGGT GCAGCACATG
    CACAGACAGA AGCCGCCCAT CATCCACAGG GACCTCAAGG TTGAAAACTT ACTGCTTAGT
    AACCAGGGGA CCATCAAGCT GTGCGACTTC GGCAGTGCCA CGACCATCTC ACACTACCCA
    GACTACAGCT GGAGCGCCCA GCGCCGGGCC CTGGTGGAGG AGGAGATCAC CAGGAACACG
    ACCCCCATGT ACAGGACGCC GGAAATTGTG GACTTGTACT CAAACTTTCC GATTGGCGAG
    AAGCAGGACA TCTGGGCCCT GGGCTGCATC CTGTACCTGC TCTGCTTCGG GCAGCACCCC
    TTTGAGGACG GAGCGAAGCT TCGCATCGTC AACGGGAAGT ACGCAATCCC TGCCGACGAC
    ACGCGGTACT CCGTGTTCCA CAGCCTCATC CGTTCCATGC TCAAGGTCAA CCCGGAGGAG
    CGGCTGTCCG TCACCGAGCT GGTCAACCAG CTCCAGGAGA TCGCGGCCGC CCGCAACGTG
    AACCCCAAGT CACCCATCAC TGAGCTCCTG GAGCAGAATG GAGGCTATGG GCACGCGGCC
    CCTCCCGGCT CCACGGGCAG CAGTCTGGTG GTCACTGAGT ACGAGCAGCC CTATGGCGGG
    TTCCTGGACA TTCTGCGTGG GGGCACCGAG CGCCTCTTCA CCAACATCAA GGACACCTCC
    TCCAAAGTCA TCCAGTCCGT TGCCAACTAT GCGAAGGGAG ATCTGGATAT TTCTTACATC
    ACGTCTAGAA TCGCAGTCAT GTCGTTCCCG GCGGAGGGCG TGGAGTCGGC CATCAAGAAC
    AACATTGAGG ATGTACGGCT GTTCCTGGAC TCGAAGCACC CGGGCCGCTA CGCCGTCTAC
    AACCTGTCCC CGCGGACCTA CCGGCCCTCG AAGTTCCACA ACCGGGTCTC CGACTGCGGC
    TGGGTGGCCC GGCGGGCCCC GAGCCTCCGC AGCCTGTACA GCCTCTGCAG GAACATGCAC
    GCCTGGCTGC GCCGCGACCC TGGCAACGTC TGCGTGGTGC ACTGCATGGA CGGGAGGGCG
    GTGTCGGCCG TGGCCGTGTG CTCCTTCTTG TGCTTCTGCC GGCTCTTCAG CACCGCGGAG
    GCCGCTGTGT ACATGTTCAG CATGAAGCGG TGCCCACCGG GCATCTGGCC ATCCCACAAG
    AGGTACATTG AGTACATGTG TGACATGGTG GCCGAGGAGC CCGTCACGCC CCACAGCAAG
    CCCATCCTGG TGAAGGCCGT GGTCATGACG CCTGTGCCGC TCTTCAGCAA GCAGAGGAGC
    GGCTGCAGGC CCTTCTGCGA GGTCTACGTG GGGGATGAGC GCGTGACCAC CACGTCCCAG
    GAATACGACA AGATGAGAGA CTTTAAAATT GAGGATGGCA AAGCGGTCAT TCCCCTGGGC
    ATAACAGTCC AGGGGGACGT GCTCATCATC ATCTATCATG CGAGGTCCAC CCTGGGAGGA
    AGGCTGCAGG CCAAGATGGC GTCCATGAAG ATGTTTCAGA TCCAGTTTCA CACAGGGTTC
    GTGCCTCGGA ACGCAGCTAC TGTGAAGTTT GCCAAGTATG ATCTGGATGC TTGCGACATC
    CAGGAGAAGT ACCCCGACTT GTTCCAAGTG AGCCTGGAGG TGGAGGTGGA GCCCAGAGAC
    AGGCCCGGCC ACGAGGCCCC GCCCTGGGAG GGTGCAGGCA TGCGAGGGCT GAACCCCAAG
    ATCCTCTTCT CCAGCCGGGA GGAGCAGCAA GATGTCCTGT CCAAGTTTGG GAAGCCAGAG
    CTCCCCAGGC AGCCTGGGTC CACCGCACAG TACGACGCCG AGGCCGGGCC CGCGGATGCC
    GAGCCCCCCG AGTCAGACTC GCCGCACAGC AGCGTGGATG CTGACCACTT CCTGCACACG
    CTGGACTGGC GGGAGGATCG GGACTCCAGT GTGGGCCCGG AGGGTCACCT GGCCGAGGAG
    GGTCCCTCTG CTGTGGCCGA GGACGGCTGT GTGAGTGAGC CATCGGACGA GGAAGCCCCG
    ACACCCTCTG CGGAGAGCGG GGACACAGGC GATGAGGACG TGCCCGCCCC CGCCGCCCCG
    CAGGACGACA GCGTGGACCT TCTGGGGCTG CACGCGGAGG CGGGGCCTGT GCCCGCCCCC
    CAGGCCCCCG GCGGCCCCCC CAGCAATGCA GACCTGCTCG GCTGCCTGCT CGGGGCCCCG
    GGCCCCGCTC CGGAGGGTGC TGTGGGGGGC CTGCTCGACG GGGATGCGGC CCTGCTCTTC
    AGCAGCCCAG CCCCCGCGCC TTGCACCCAG ACAACTGCCC CGGCCGCTGA CCCGTTCGGC
    CCGCTCCTGC TGCCGCCCGA CGTGGACACC CCTCCTAGCT CGCAGCCTGA CCTCTTCGGG
    GAATTCCTCA ACTCAGACGC CCCGGCCGCC CAGCCCGCGC CCTTCCCCTC CACCCACAGC
    GCTCCGCCGC CAGCCTGCAG CACCGACTTC CTGCATCTTG GGGACCTGCC CGCCGAGCCC
    AGCAAGATGA CAGCCTCTGC CAGCCACCCC GACCTGCTGG GAGCCTGGGA TGCCTGGGCT
    GAGGCGCCGG CCCCCGCGCT GGCTGCAGAC GGCACCTTCT TCTCTCCTGG AGACCAGCCA
    GCCCCTTCTG GCCTTCCGGC TGGCCAGACC AAGTCTCAGA GCCAGGACCC GTTTGCAGAC
    CTTGGCGACC TCAGTGCCGG CCTCCAAGGC TCGCCTGCCG CAGCTGCTCC TGGGGGCTTT
    GGTCCCAAAG CAGCGCCCCC TCCCAAGGCC AGCAGCTCCT GGCAGACGAG TCGGCCCCCA
    GCCCAGGGCA CCCCGTGGCC CCCCCAGGCC AAGCCGCCCC CCAAAGCCAG TGTACCGCCA
    AGGCCTAACT ACACCTCCAG CTTCAGTGTG ATCGGTAGCC GGGAGGAGCG GGGGGTCCGC
    GCCCCCAGCT TTGGTCCAAA GCCGAGGGTG TCAGAAAACG ACTTTGAAGA CCTGCTCTCC
    AACCAGGGCT TCTCCTCGCG GGCTGACAGG AAGGGGCCGC GGACCATCGC TGAGATGAGG
    AGGCAGGACC AGGCCCGGGA CTTGGATCCG CTGAAGCTGA AGCTTCTGGA GTGGACGGAG
    GGCAAGGAGA GGAACATCCG CGCCCTGCTG TCCACGCTGC ACACGGCGCT GTGGGACGGC
    GAGAGCCGCT GGACGCCGGT GGGCATGGCC GACCTGGTGA CGCCCGCACA AGTGAAGAAG
    CACTACCGCC GTGCCGTGCT GGTGGTCCAC CCCGACAAGG CCCGGGGGCA GCCCTACGAG
    CAGTATGCCC GGATGATCTT CATGGAGCTC AACGATGCGT GGGCTGAGTT TGAGAGCCAG
    GGCTCCCGGC CACTCTTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa47972
    Clone ID Related Accession (Same CDS sequence) XM_010806359.2
    Accession Version XM_010806359.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3417bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product cyclin-G-associated kinase isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103986.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010806359.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGTCGCTGC TGCAGTCCGC GCTCGACTTC CTGGCGGGCC CGGGCTCCCT GGGGGGCGCG 
    GCCGGCCGCG ACCAGACGGA CTTCGTGGGG CAGACGGTGG AGATGGGCGA GCTGCGGCTG
    CGGGTGCGCC GGGTCCTGGC TGAAGGAGGG TTTGCATTTG TGTATGAAGC TCAAGATCTG
    GGAAGTGGCA GAGAGTATGC ATTGAAGAGA CTGCTGTCCA ACGAAGAGGA GAAGAACAGA
    GCCATCATTC AGGAAGTGTG TTTCATGAAA AAACTTTCAG GCCACCCAAA CATTGTCCAG
    TTTTGTTCTG CAGCATCGAT AGGAAAAGAG GAGTCGGACA CTGGGCAGGC GGAGTTCCTG
    CTGCTCATGG AGCTCTGCAG AGGGCAGCTG GTGGAATTTT TAAAAAAAGT TGAATCGAAA
    GGTCCGCTCT CGTGCGACAC CGTCCTGAAG ATCTTCTACC AGACGTGCCG GGCGGTGCAG
    CACATGCACA GACAGAAGCC GCCCATCATC CACAGGGACC TCAAGGTTGA AAACTTACTG
    CTTAGTAACC AGGGGACCAT CAAGCTGTGC GACTTCGGCA GTGCCACGAC CATCTCACAC
    TACCCAGACT ACAGCTGGAG CGCCCAGCGC CGGGCCCTGG TGGAGGAGGA GATCACCAGG
    AACACGACCC CCATGTACAG GACGCCGGAA ATTGTGGACT TGTACTCAAA CTTTCCGATT
    GGCGAGAAGC AGGACATCTG GGCCCTGGGC TGCATCCTGT ACCTGCTCTG CTTCGGGCAG
    CACCCCTTTG AGGACGGAGC GAAGCTTCGC ATCGTCAACG GGAAGTACGC AATCCCTGCC
    GACGACACGC GGTACTCCGT GTTCCACAGC CTCATCCGTT CCATGCTCAA GGTCAACCCG
    GAGGAGCGGC TGTCCGTCAC CGAGCTGGTC AACCAGCTCC AGGAGATCGC GGCCGCCCGC
    AACGTGAACC CCAAGTCACC CATCACTGAG CTCCTGGAGC AGAATGGAGG CTATGGGCAC
    GCGGCCCCTC CCGGCTCCAC GGGCAGCAGT CTGGTGGTCA CTGAGTACGA GCAGCCCTAT
    GGCGGGTTCC TGGACATTCT GCGTGGGGGC ACCGAGCGCC TCTTCACCAA CATCAAGGAC
    ACCTCCTCCA AAGTCATCCA GTCCGTTGCC AACTATGCGA AGGGAGATCT GGATATTTCT
    TACATCACGT CTAGAATCGC AGTCATGTCG TTCCCGGCGG AGGGCGTGGA GTCGGCCATC
    AAGAACAACA TTGAGGATGT ACGGCTGTTC CTGGACTCGA AGCACCCGGG CCGCTACGCC
    GTCTACAACC TGTCCCCGCG GACCTACCGG CCCTCGAAGT TCCACAACCG GGTCTCCGAC
    TGCGGCTGGG TGGCCCGGCG GGCCCCGAGC CTCCGCAGCC TGTACAGCCT CTGCAGGAAC
    ATGCACGCCT GGCTGCGCCG CGACCCTGGC AACGTCTGCG TGGTGCACTG CATGGACGGG
    AGGGCGGTGT CGGCCGTGGC CGTGTGCTCC TTCTTGTGCT TCTGCCGGCT CTTCAGCACC
    GCGGAGGCCG CTGTGTACAT GTTCAGCATG AAGCGGTGCC CACCGGGCAT CTGGCCATCC
    CACAAGAGGT ACATTGAGTA CATGTGTGAC ATGGTGGCCG AGGAGCCCGT CACGCCCCAC
    AGCAAGCCCA TCCTGGTGAA GGCCGTGGTC ATGACGCCTG TGCCGCTCTT CAGCAAGCAG
    AGGAGCGGCT GCAGGCCCTT CTGCGAGGTC TACGTGGGGG ATGAGCGCGT GACCACCACG
    TCCCAGGAAT ACGACAAGAT GAGAGACTTT AAAATTGAGG ATGGCAAAGC GGTCATTCCC
    CTGGGCATAA CAGTCCAGGG GGACGTGCTC ATCATCATCT ATCATGCGAG GTCCACCCTG
    GGAGGAAGGC TGCAGGCCAA GATGGCGTCC ATGAAGATGT TTCAGATCCA GTTTCACACA
    GGGTTCGTGC CTCGGAACGC AGCTACTGTG AAGTTTGCCA AGTATGATCT GGATGCTTGC
    GACATCCAGG AGAAGTACCC CGACTTGTTC CAAGTGAGCC TGGAGGTGGA GGTGGAGCCC
    AGAGACAGGC CCGGCCACGA GGCCCCGCCC TGGGAGGGTG CAGGCATGCG AGGGCTGAAC
    CCCAAGATCC TCTTCTCCAG CCGGGAGGAG CAGCAAGATG TCCTGTCCAA GTTTGGGAAG
    CCAGAGCTCC CCAGGCAGCC TGGGTCCACC GCACAGTACG ACGCCGAGGC CGGGCCCGCG
    GATGCCGAGC CCCCCGAGTC AGACTCGCCG CACAGCAGCG TGGATGCTGA CCACTTCCTG
    CACACGCTGG ACTGGCGGGA GGATCGGGAC TCCAGTGTGG GCCCGGAGGG TCACCTGGCC
    GAGGAGGGTC CCTCTGCTGT GGCCGAGGAC GGCTGTGTGA GTGAGCCATC GGACGAGGAA
    GCCCCGACAC CCTCTGCGGA GAGCGGGGAC ACAGGCGATG AGGACGTGCC CGCCCCCGCC
    GCCCCGCAGG ACGACAGCGT GGACCTTCTG GGGCTGCACG CGGAGGCGGG GCCTGTGCCC
    GCCCCCCAGG CCCCCGGCGG CCCCCCCAGC AATGCAGACC TGCTCGGCTG CCTGCTCGGG
    GCCCCGGGCC CCGCTCCGGA GGGTGCTGTG GGGGGCCTGC TCGACGGGGA TGCGGCCCTG
    CTCTTCAGCA GCCCAGCCCC CGCGCCTTGC ACCCAGACAA CTGCCCCGGC CGCTGACCCG
    TTCGGCCCGC TCCTGCTGCC GCCCGACGTG GACACCCCTC CTAGCTCGCA GCCTGACCTC
    TTCGGGGAAT TCCTCAACTC AGACGCCCCG GCCGCCCAGC CCGCGCCCTT CCCCTCCACC
    CACAGCGCTC CGCCGCCAGC CTGCAGCACC GACTTCCTGC ATCTTGGGGA CCTGCCCGCC
    GAGCCCAGCA AGATGACAGC CTCTGCCAGC CACCCCGACC TGCTGGGAGC CTGGGATGCC
    TGGGCTGAGG CGCCGGCCCC CGCGCTGGCT GCAGACGGCA CCTTCTTCTC TCCTGGAGAC
    CAGCCAGCCC CTTCTGGCCT TCCGGCTGGC CAGACCAAGT CTCAGAGCCA GGACCCGTTT
    GCAGACCTTG GCGACCTCAG TGCCGGCCTC CAAGGGCCCA GCAACAGCAG CAGATGCCCT
    TCTGCCTTCT CTCAGGAGCT TGTTGCCAAG ACAACGCCAG GAGCTGCCAG AGCAGGCACA
    GGCACTGGCT CCTCTGCCAG CTCCATGGAG TGTGGGGGCC AGCTGGGGGC TGCCTCAGCC
    CAGGATGGTG GAACCTGCCT GCCCCGCTTG GAGTGTGGGG CCGGGGTGCA GAATTCACCA
    CCATCACTGT CCTTTCTGAG AAGCCCTCTA AGGACGTTGG TTCTTTGGAG GCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010804661.1
    CDS114..3530
    Translation

    Target ORF information:

    RefSeq Version XM_010806359.2
    Organism Bos taurus(cattle)
    Definition Bos taurus cyclin G associated kinase (GAK), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806359.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGTCGCTGC TGCAGTCCGC GCTCGACTTC CTGGCGGGCC CGGGCTCCCT GGGGGGCGCG 
    GCCGGCCGCG ACCAGACGGA CTTCGTGGGG CAGACGGTGG AGATGGGCGA GCTGCGGCTG
    CGGGTGCGCC GGGTCCTGGC TGAAGGAGGG TTTGCATTTG TGTATGAAGC TCAAGATCTG
    GGAAGTGGCA GAGAGTATGC ATTGAAGAGA CTGCTGTCCA ACGAAGAGGA GAAGAACAGA
    GCCATCATTC AGGAAGTGTG TTTCATGAAA AAACTTTCAG GCCACCCAAA CATTGTCCAG
    TTTTGTTCTG CAGCATCGAT AGGAAAAGAG GAGTCGGACA CTGGGCAGGC GGAGTTCCTG
    CTGCTCATGG AGCTCTGCAG AGGGCAGCTG GTGGAATTTT TAAAAAAAGT TGAATCGAAA
    GGTCCGCTCT CGTGCGACAC CGTCCTGAAG ATCTTCTACC AGACGTGCCG GGCGGTGCAG
    CACATGCACA GACAGAAGCC GCCCATCATC CACAGGGACC TCAAGGTTGA AAACTTACTG
    CTTAGTAACC AGGGGACCAT CAAGCTGTGC GACTTCGGCA GTGCCACGAC CATCTCACAC
    TACCCAGACT ACAGCTGGAG CGCCCAGCGC CGGGCCCTGG TGGAGGAGGA GATCACCAGG
    AACACGACCC CCATGTACAG GACGCCGGAA ATTGTGGACT TGTACTCAAA CTTTCCGATT
    GGCGAGAAGC AGGACATCTG GGCCCTGGGC TGCATCCTGT ACCTGCTCTG CTTCGGGCAG
    CACCCCTTTG AGGACGGAGC GAAGCTTCGC ATCGTCAACG GGAAGTACGC AATCCCTGCC
    GACGACACGC GGTACTCCGT GTTCCACAGC CTCATCCGTT CCATGCTCAA GGTCAACCCG
    GAGGAGCGGC TGTCCGTCAC CGAGCTGGTC AACCAGCTCC AGGAGATCGC GGCCGCCCGC
    AACGTGAACC CCAAGTCACC CATCACTGAG CTCCTGGAGC AGAATGGAGG CTATGGGCAC
    GCGGCCCCTC CCGGCTCCAC GGGCAGCAGT CTGGTGGTCA CTGAGTACGA GCAGCCCTAT
    GGCGGGTTCC TGGACATTCT GCGTGGGGGC ACCGAGCGCC TCTTCACCAA CATCAAGGAC
    ACCTCCTCCA AAGTCATCCA GTCCGTTGCC AACTATGCGA AGGGAGATCT GGATATTTCT
    TACATCACGT CTAGAATCGC AGTCATGTCG TTCCCGGCGG AGGGCGTGGA GTCGGCCATC
    AAGAACAACA TTGAGGATGT ACGGCTGTTC CTGGACTCGA AGCACCCGGG CCGCTACGCC
    GTCTACAACC TGTCCCCGCG GACCTACCGG CCCTCGAAGT TCCACAACCG GGTCTCCGAC
    TGCGGCTGGG TGGCCCGGCG GGCCCCGAGC CTCCGCAGCC TGTACAGCCT CTGCAGGAAC
    ATGCACGCCT GGCTGCGCCG CGACCCTGGC AACGTCTGCG TGGTGCACTG CATGGACGGG
    AGGGCGGTGT CGGCCGTGGC CGTGTGCTCC TTCTTGTGCT TCTGCCGGCT CTTCAGCACC
    GCGGAGGCCG CTGTGTACAT GTTCAGCATG AAGCGGTGCC CACCGGGCAT CTGGCCATCC
    CACAAGAGGT ACATTGAGTA CATGTGTGAC ATGGTGGCCG AGGAGCCCGT CACGCCCCAC
    AGCAAGCCCA TCCTGGTGAA GGCCGTGGTC ATGACGCCTG TGCCGCTCTT CAGCAAGCAG
    AGGAGCGGCT GCAGGCCCTT CTGCGAGGTC TACGTGGGGG ATGAGCGCGT GACCACCACG
    TCCCAGGAAT ACGACAAGAT GAGAGACTTT AAAATTGAGG ATGGCAAAGC GGTCATTCCC
    CTGGGCATAA CAGTCCAGGG GGACGTGCTC ATCATCATCT ATCATGCGAG GTCCACCCTG
    GGAGGAAGGC TGCAGGCCAA GATGGCGTCC ATGAAGATGT TTCAGATCCA GTTTCACACA
    GGGTTCGTGC CTCGGAACGC AGCTACTGTG AAGTTTGCCA AGTATGATCT GGATGCTTGC
    GACATCCAGG AGAAGTACCC CGACTTGTTC CAAGTGAGCC TGGAGGTGGA GGTGGAGCCC
    AGAGACAGGC CCGGCCACGA GGCCCCGCCC TGGGAGGGTG CAGGCATGCG AGGGCTGAAC
    CCCAAGATCC TCTTCTCCAG CCGGGAGGAG CAGCAAGATG TCCTGTCCAA GTTTGGGAAG
    CCAGAGCTCC CCAGGCAGCC TGGGTCCACC GCACAGTACG ACGCCGAGGC CGGGCCCGCG
    GATGCCGAGC CCCCCGAGTC AGACTCGCCG CACAGCAGCG TGGATGCTGA CCACTTCCTG
    CACACGCTGG ACTGGCGGGA GGATCGGGAC TCCAGTGTGG GCCCGGAGGG TCACCTGGCC
    GAGGAGGGTC CCTCTGCTGT GGCCGAGGAC GGCTGTGTGA GTGAGCCATC GGACGAGGAA
    GCCCCGACAC CCTCTGCGGA GAGCGGGGAC ACAGGCGATG AGGACGTGCC CGCCCCCGCC
    GCCCCGCAGG ACGACAGCGT GGACCTTCTG GGGCTGCACG CGGAGGCGGG GCCTGTGCCC
    GCCCCCCAGG CCCCCGGCGG CCCCCCCAGC AATGCAGACC TGCTCGGCTG CCTGCTCGGG
    GCCCCGGGCC CCGCTCCGGA GGGTGCTGTG GGGGGCCTGC TCGACGGGGA TGCGGCCCTG
    CTCTTCAGCA GCCCAGCCCC CGCGCCTTGC ACCCAGACAA CTGCCCCGGC CGCTGACCCG
    TTCGGCCCGC TCCTGCTGCC GCCCGACGTG GACACCCCTC CTAGCTCGCA GCCTGACCTC
    TTCGGGGAAT TCCTCAACTC AGACGCCCCG GCCGCCCAGC CCGCGCCCTT CCCCTCCACC
    CACAGCGCTC CGCCGCCAGC CTGCAGCACC GACTTCCTGC ATCTTGGGGA CCTGCCCGCC
    GAGCCCAGCA AGATGACAGC CTCTGCCAGC CACCCCGACC TGCTGGGAGC CTGGGATGCC
    TGGGCTGAGG CGCCGGCCCC CGCGCTGGCT GCAGACGGCA CCTTCTTCTC TCCTGGAGAC
    CAGCCAGCCC CTTCTGGCCT TCCGGCTGGC CAGACCAAGT CTCAGAGCCA GGACCCGTTT
    GCAGACCTTG GCGACCTCAG TGCCGGCCTC CAAGGGCCCA GCAACAGCAG CAGATGCCCT
    TCTGCCTTCT CTCAGGAGCT TGTTGCCAAG ACAACGCCAG GAGCTGCCAG AGCAGGCACA
    GGCACTGGCT CCTCTGCCAG CTCCATGGAG TGTGGGGGCC AGCTGGGGGC TGCCTCAGCC
    CAGGATGGTG GAACCTGCCT GCCCCGCTTG GAGTGTGGGG CCGGGGTGCA GAATTCACCA
    CCATCACTGT CCTTTCTGAG AAGCCCTCTA AGGACGTTGG TTCTTTGGAG GCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.