aux cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	aux
Entrez Gene ID	40527
Full Name	auxilin
Synonyms	Aux,Auxillin/DNAJC6,CG1107,DNAJC6,Dmel\CG1107,GAK,anon-WO0118547.393,auxillin,dAux
General protein information	Preferred Names auxilin Names CG1107 gene product from transcript CG1107-RA CG1107-PA CG1107-PB CG1107-PC CG1107-PD aux-PA aux-PB aux-PC aux-PD auxillin
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 82A1-82A1

mRNA and Protein(s)

mRNA	Protein	Name
NM_164317.3	NP_730785.1	auxilin, isoform A
NM_001275314.1	NP_001262243.1	auxilin, isoform C
NM_141181.3	NP_649438.1	auxilin, isoform B
NM_001275315.1	NP_001262244.1	auxilin, isoform D

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Gak	NP_112292.1
Homo sapiens (human)	GAK	NP_005246.2
Bos taurus (cattle)	GAK	NP_001039549.1
Danio rerio (zebrafish)	gak	XP_003198642.2
Pan troglodytes (chimpanzee)	GAK	XP_001140835.1
Mus musculus (house mouse)	Gak	NP_705797.1
Gallus gallus (chicken)	GAK	XP_424873.4
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003715	XP_310244.7
	GAK	XP_001094120.2
Canis lupus familiaris (dog)	GAK	XP_536279.4
Drosophila melanogaster (fruit fly)	aux	NP_001262243.1
Danio rerio (zebrafish)	LOC100151158	XP_001919224.1
Xenopus tropicalis (tropical clawed frog)	gak	NP_001016350.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Drosophila Rab11 and auxilin mutants to test the ligand recycling hypothesis.
Title: The functions of auxilin and Rab11 in Drosophila suggest that the fundamental role of ligand endocytosis in notch signaling cells is not recycling.

Aux participates in forming these Golgi-derived clathrin-positive vesicles and therefore, has a role in the secretory pathway.
Title: Auxilin is required for formation of Golgi-derived clathrin-coated vesicles during Drosophila spermatogenesis.

ligand endocytosis is critical for Notch signaling and auxilin participates in Notch signaling by facilitating ligand internalization
Title: The clathrin-binding motif and the J-domain of Drosophila Auxilin are essential for facilitating Notch ligand endocytosis.

Auxilin, an endocytic factor that regulates Clathrin dynamics, is also essential for Notch signaling.
Title: Auxilin is essential for Delta signaling.

The data suggest that auxilin is an integral component of the Notch signaling pathway, participating in the ubiquitin-dependent endocytosis of Delta.
Title: Drosophila melanogaster auxilin regulates the internalization of Delta to control activity of the Notch signaling pathway.

The following aux gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the aux cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa03788	NM_164317.3 Latest version!	Drosophila melanogaster auxilin (aux), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa03788	NM_141181.3 Latest version!	Drosophila melanogaster auxilin (aux), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa07849	NM_001275314.1 Latest version!	Drosophila melanogaster auxilin (aux), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa07850	NM_001275315.1 Latest version!	Drosophila melanogaster auxilin (aux), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

aux ( NM_164317.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_730785.1 Drosophila melanogaster auxilin (aux), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa03788
Clone ID Related Accession (Same CDS sequence)	NM_164317.3 , NM_141181.3
Accession Version	NM_164317.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3498bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	auxilin, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_164317.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGCGAGT TCTTTAAGTC GCTCAACCTC AACTACTTCT CCTCTAGCGA GGTCAACGGA 
GCTGCAGGAA ACGGAGCACC AGAGGCCCTC GGTGGCAGGC TGGACAATGA CTTCGTGGGC 
CAGGTCGTTG AAGTCGCTGG ACACAGGCTG CGCATCAAGT GTGTCATCGC CGAAGGCGGA 
TACGCCTTCG TGTATGTAGC CCAAGATGTG CAAACTGGCA CAGAATACGC CCTCAAGCGT 
CTGATCGGGG CTGACATGCA AGCCTCCACC GCCATCATCA ACGAGATCAA CATCCACAAG 
CAGCTGTCGG GGCACGAGAA CATTGTCGCC TTTGTGGGTT CCAGTTATAC CGCCCCATCA 
ACTCAATTGG GAGCCCAGTA CTTGCTCCTC ACCGAGCTGT GTAAAGGCGG ATCTCTGGTG 
GACTGCTTCA GAACAAACAA TGCTCCATTC AATCCGACTT GTGTTCTGCG CATCTTCTAC 
CAAATGGCGC GGGCTGTGGC CAGCTTGCAC TCACAGTCAC CGCCGATAGC CCACCGAGAT 
ATAAAGATTG AGAACTTTCT CATTGGCAAC GACAAACAGA TCAAGCTGTG CGACTTTGGG 
TCCGCCAGCA CGGAGGTCCT GTCGCCCACG TTTGAGTGGA GCGCCAACCA GCGCAGCATG 
CTGGAGGACC AGCTAAACAC TGTTACTACT CCGATGTACC GATCCCCTGA GATGCTCGAC 
ACTTGGTCCA ACAACCCGAT TGGGCCCAAA GTGGACATAT GGGCTCTCGG ATGCATTCTC 
TACTTCCTGT GCTACCGCAA GCATCCCTAC GAGGATGGCG GAAAGCTGCG GATCATTAAC 
GCCAATTACA TGCTTCCGCC AGATCCTCAG TACCAGTGCT TCAGCGATAT AATCCGTGGC 
TGTTTAAAAG TGAACCCGTT CGAGCGCCTG GATATTACTA TGGTGCTGGA GGGCCTTGCA 
GCCCTCGCTG AGACACACAA TTGGTCGCTC AAAGGACCTC TAGACCTTCA TATAATGCCC 
ATCGAGTCCC TTCCGGCCGA AAGTCCTCTC AAAAGTTCTG CTTACTCTGA GGTTTACACC 
GAACCGCTTT CTGCCGCCAT ACCATCATCT TATAATGGAC ACGGCAGTCT TCTGTCGTCA 
CTGCGTGGTG GCGCTGGAAC GCTTTTAAAG AACATTAAAG ATACGTCCAC CAAAGTGATG 
CAAACAATGC AGCAATCTCT TGCTCGCAAC GATCTTGATA TAAGCCACAT TACCTCTCGC 
ATCCTGGTAA TGCCCTGCCC CTCTGACGGA TTCGAGTCGA CGTACAAGAC GAACAACATC 
GAAGACGTCC GACTATCTCT GGAGAGCCGA TTTGTCCCAC AAAAATTAAG CATTTACAAC 
TTTGGCCAAC GAACAGAGCC ACGGCTACCG CCACCCGTGC GAACAGTTGA GGCAGGTTCG 
GTTTATGGGT GCCCACAGGC ACATGCTCCT AATCTGCAGG GCCTTTTTAC CGTATCAGCC 
GACATGTACA ATTTTCTAAA TGCGGACCCC AAATCTGTCG TTATCGTGCA GACTGGAGAC 
TCGGGTGGAT GCACTGCTGC CACCGTTATA TGCGCGCTTC TTATGTATGC AGATTTGCTG 
CGGGAGCCGG AGGACGCGGT GCAAGTCTTC GCCGTTAAGC GGCATACCAT TAACCTCCGC 
CCCTCCGAGT TCCGTTACCT GTATTACTTC GGTGACATCC TGCGGCCCAC GCCCCTGCTG 
CCGCACTATA AGAACACAAC CCTTGTTTCA CTCAGCTGCC AGCCTGTGCC CAGAATGACC 
AAGGCACGCG ACGGCTGTAG GATCTATATG GAGGTTTATT GTAATGGAAA CTTGCTACTC 
AGCACTCTAC AGGACTATGA AAAAATGCGG TTGTATCAGG CCGGCCCCGG CAAAATAGTT 
CTGCCGATTA ACTTGACCGC ATGCGGTGAT GTGACAGTGG TATTATTTCA CGCCCGCAAA 
GGAATGGTGC GACCACAAGG CCTCAAGATA TGTCAGTTCC AATTCAACAC GGGATTCATC 
CCCGAACCAG AAACGTTGAT TACATTCACA AACCAGGACT TGGATGATCT GCCAGACCCT 
GAGCAGGTGA CACCTCGGTT CTGCGTTTCA CTTTCTCTGG CTGTTACTGA CTCGGAATCA 
CCTCCCAGCC ATAAGCCACC ATGGATGCCA GCCAAGCCCA AGCGGTCACC CGCCGCTTTG 
TTCAGCTCAG ATTTGGAGTA TGCAGAAATG CTCGACAATT TTGTAACCAA GCCTAGCACG 
CGTTCCTCAC CACCACCAGG TGCCCGCAAG AGTGATCGCG TCAGCTCTCC GCTCGTTTTG 
CCCGACGTCA CGGAGACCGC CCACGAACTG CCTTCGCCCA TGCCAGAGCC ATCTCCGCCC 
ATAGACTTGC TCAATTTAAA CCAGCAGCCC AGTGACGTAC CAGCAGCAGA TCCGTTAACC 
AGCGCTAAGC CCAGCACAGA TGCCAGCTTT GACTTGCTAG GGGCCTTCGG TGATGATGAC 
TCTACGGGCA TAGGATCTGC GCCAATTCCT GACATACTGC CACCACCGCC ACTGCAGCAG 
CCGCAACCAA AAATAAACAA TGACCCATTC GACATATTTG GATCTGTGGA CCAAGGTGGC 
GTGCCCAGCA TGAAGCCATC AGGCTTTCCA CCTTTCGTTG GCTCCTTACC AACTTTTGTT 
GCTCAGCCAG CGGCTACCAC CAATCCTTCA CCAACTCATC CGGCAAGGGC ATCTGCAGAT 
CCATTTGCGA ACATCGCCGA CCTGGCGACA GGGCTCAACT TAAACTTTAA CCGCAGCACG 
CTAAGTGGAA AGTCTCCCGT CAACACTAGT CCGCAGCCTA CTCAGTTCTC AAGCCCCACT 
CACAAGCCAT CGCCATCCTC GCAACCACAA GCAACGTTTA TGCATACACC GCCGACACCG 
CAGACTCTGC CCTCGACATC GTCTATCAGG ACGCCGACGC AGCCACAGGC GGTACCTGCT 
CAATCAAGAC CAGACTACAG TCGACTTCAC TTTGATTCTC AGAAAGCAGC CCAGCAACCG 
GGCACTGGCG GGTCCAAGAA CTCTGACATA TTTGCTGACA TTCTGGGCCA ACAAGGATAC 
TCCTTTGGCA GCAAAATGAA CCAGTGTCCA CGATCCATCA ATGAAATGCG CAAGGAAGAT 
TTGTTCAAGG ATATGGACCC GAAGAAAGTG CGGATTATGG AATGGACTGA TGGTAAGAAA 
AACAACATCC GTGCGCTTCT CTGCTCTATG CACACAGTGT TGTGGGACAA TGCCAAGTGG 
CAGCGCTGCG AGATGTCCAC CATGGTGACA CCGACCGAGG TTAAGAAAGC ATACCGTCGC 
GCCTGCCTAG CAGTGCATCC TGATAAGCAT AATGGAACAG AGAATGAAGA GATTGCCAAG 
CTTATATTTA TGGAACTAAA CAACGCATGG ACAGATTTTG AAAACGATGC CACGCAGCAA 
AATATGTTTA ATGCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_730785.1
CDS	256..3753
Translation	MGEFFKSLNLNYFSSSEVNGAAGNGAPEALGGRLDNDFVGQVVEVAGHRLRIKCVIAEGGYAFVYVAQDVQTGTEYALKRLIGADMQASTAIINEINIHKQLSGHENIVAFVGSSYTAPSTQLGAQYLLLTELCKGGSLVDCFRTNNAPFNPTCVLRIFYQMARAVASLHSQSPPIAHRDIKIENFLIGNDKQIKLCDFGSASTEVLSPTFEWSANQRSMLEDQLNTVTTPMYRSPEMLDTWSNNPIGPKVDIWALGCILYFLCYRKHPYEDGGKLRIINANYMLPPDPQYQCFSDIIRGCLKVNPFERLDITMVLEGLAALAETHNWSLKGPLDLHIMPIESLPAESPLKSSAYSEVYTEPLSAAIPSSYNGHGSLLSSLRGGAGTLLKNIKDTSTKVMQTMQQSLARNDLDISHITSRILVMPCPSDGFESTYKTNNIEDVRLSLESRFVPQKLSIYNFGQRTEPRLPPPVRTVEAGSVYGCPQAHAPNLQGLFTVSADMYNFLNADPKSVVIVQTGDSGGCTAATVICALLMYADLLREPEDAVQVFAVKRHTINLRPSEFRYLYYFGDILRPTPLLPHYKNTTLVSLSCQPVPRMTKARDGCRIYMEVYCNGNLLLSTLQDYEKMRLYQAGPGKIVLPINLTACGDVTVVLFHARKGMVRPQGLKICQFQFNTGFIPEPETLITFTNQDLDDLPDPEQVTPRFCVSLSLAVTDSESPPSHKPPWMPAKPKRSPAALFSSDLEYAEMLDNFVTKPSTRSSPPPGARKSDRVSSPLVLPDVTETAHELPSPMPEPSPPIDLLNLNQQPSDVPAADPLTSAKPSTDASFDLLGAFGDDDSTGIGSAPIPDILPPPPLQQPQPKINNDPFDIFGSVDQGGVPSMKPSGFPPFVGSLPTFVAQPAATTNPSPTHPARASADPFANIADLATGLNLNFNRSTLSGKSPVNTSPQPTQFSSPTHKPSPSSQPQATFMHTPPTPQTLPSTSSIRTPTQPQAVPAQSRPDYSRLHFDSQKAAQQPGTGGSKNSDIFADILGQQGYSFGSKMNQCPRSINEMRKEDLFKDMDPKKVRIMEWTDGKKNNIRALLCSMHTVLWDNAKWQRCEMSTMVTPTEVKKAYRRACLAVHPDKHNGTENEEIAKLIFMELNNAWTDFENDATQQNMFNA

Target ORF information:

RefSeq Version	NM_164317.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster auxilin (aux), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_164317.3

ORF Insert Sequence:

ATGGGCGAGT TCTTTAAGTC GCTCAACCTC AACTACTTCT CCTCTAGCGA GGTCAACGGA 
GCTGCAGGAA ACGGAGCACC AGAGGCCCTC GGTGGCAGGC TGGACAATGA CTTCGTGGGC 
CAGGTCGTTG AAGTCGCTGG ACACAGGCTG CGCATCAAGT GTGTCATCGC CGAAGGCGGA 
TACGCCTTCG TGTATGTAGC CCAAGATGTG CAAACTGGCA CAGAATACGC CCTCAAGCGT 
CTGATCGGGG CTGACATGCA AGCCTCCACC GCCATCATCA ACGAGATCAA CATCCACAAG 
CAGCTGTCGG GGCACGAGAA CATTGTCGCC TTTGTGGGTT CCAGTTATAC CGCCCCATCA 
ACTCAATTGG GAGCCCAGTA CTTGCTCCTC ACCGAGCTGT GTAAAGGCGG ATCTCTGGTG 
GACTGCTTCA GAACAAACAA TGCTCCATTC AATCCGACTT GTGTTCTGCG CATCTTCTAC 
CAAATGGCGC GGGCTGTGGC CAGCTTGCAC TCACAGTCAC CGCCGATAGC CCACCGAGAT 
ATAAAGATTG AGAACTTTCT CATTGGCAAC GACAAACAGA TCAAGCTGTG CGACTTTGGG 
TCCGCCAGCA CGGAGGTCCT GTCGCCCACG TTTGAGTGGA GCGCCAACCA GCGCAGCATG 
CTGGAGGACC AGCTAAACAC TGTTACTACT CCGATGTACC GATCCCCTGA GATGCTCGAC 
ACTTGGTCCA ACAACCCGAT TGGGCCCAAA GTGGACATAT GGGCTCTCGG ATGCATTCTC 
TACTTCCTGT GCTACCGCAA GCATCCCTAC GAGGATGGCG GAAAGCTGCG GATCATTAAC 
GCCAATTACA TGCTTCCGCC AGATCCTCAG TACCAGTGCT TCAGCGATAT AATCCGTGGC 
TGTTTAAAAG TGAACCCGTT CGAGCGCCTG GATATTACTA TGGTGCTGGA GGGCCTTGCA 
GCCCTCGCTG AGACACACAA TTGGTCGCTC AAAGGACCTC TAGACCTTCA TATAATGCCC 
ATCGAGTCCC TTCCGGCCGA AAGTCCTCTC AAAAGTTCTG CTTACTCTGA GGTTTACACC 
GAACCGCTTT CTGCCGCCAT ACCATCATCT TATAATGGAC ACGGCAGTCT TCTGTCGTCA 
CTGCGTGGTG GCGCTGGAAC GCTTTTAAAG AACATTAAAG ATACGTCCAC CAAAGTGATG 
CAAACAATGC AGCAATCTCT TGCTCGCAAC GATCTTGATA TAAGCCACAT TACCTCTCGC 
ATCCTGGTAA TGCCCTGCCC CTCTGACGGA TTCGAGTCGA CGTACAAGAC GAACAACATC 
GAAGACGTCC GACTATCTCT GGAGAGCCGA TTTGTCCCAC AAAAATTAAG CATTTACAAC 
TTTGGCCAAC GAACAGAGCC ACGGCTACCG CCACCCGTGC GAACAGTTGA GGCAGGTTCG 
GTTTATGGGT GCCCACAGGC ACATGCTCCT AATCTGCAGG GCCTTTTTAC CGTATCAGCC 
GACATGTACA ATTTTCTAAA TGCGGACCCC AAATCTGTCG TTATCGTGCA GACTGGAGAC 
TCGGGTGGAT GCACTGCTGC CACCGTTATA TGCGCGCTTC TTATGTATGC AGATTTGCTG 
CGGGAGCCGG AGGACGCGGT GCAAGTCTTC GCCGTTAAGC GGCATACCAT TAACCTCCGC 
CCCTCCGAGT TCCGTTACCT GTATTACTTC GGTGACATCC TGCGGCCCAC GCCCCTGCTG 
CCGCACTATA AGAACACAAC CCTTGTTTCA CTCAGCTGCC AGCCTGTGCC CAGAATGACC 
AAGGCACGCG ACGGCTGTAG GATCTATATG GAGGTTTATT GTAATGGAAA CTTGCTACTC 
AGCACTCTAC AGGACTATGA AAAAATGCGG TTGTATCAGG CCGGCCCCGG CAAAATAGTT 
CTGCCGATTA ACTTGACCGC ATGCGGTGAT GTGACAGTGG TATTATTTCA CGCCCGCAAA 
GGAATGGTGC GACCACAAGG CCTCAAGATA TGTCAGTTCC AATTCAACAC GGGATTCATC 
CCCGAACCAG AAACGTTGAT TACATTCACA AACCAGGACT TGGATGATCT GCCAGACCCT 
GAGCAGGTGA CACCTCGGTT CTGCGTTTCA CTTTCTCTGG CTGTTACTGA CTCGGAATCA 
CCTCCCAGCC ATAAGCCACC ATGGATGCCA GCCAAGCCCA AGCGGTCACC CGCCGCTTTG 
TTCAGCTCAG ATTTGGAGTA TGCAGAAATG CTCGACAATT TTGTAACCAA GCCTAGCACG 
CGTTCCTCAC CACCACCAGG TGCCCGCAAG AGTGATCGCG TCAGCTCTCC GCTCGTTTTG 
CCCGACGTCA CGGAGACCGC CCACGAACTG CCTTCGCCCA TGCCAGAGCC ATCTCCGCCC 
ATAGACTTGC TCAATTTAAA CCAGCAGCCC AGTGACGTAC CAGCAGCAGA TCCGTTAACC 
AGCGCTAAGC CCAGCACAGA TGCCAGCTTT GACTTGCTAG GGGCCTTCGG TGATGATGAC 
TCTACGGGCA TAGGATCTGC GCCAATTCCT GACATACTGC CACCACCGCC ACTGCAGCAG 
CCGCAACCAA AAATAAACAA TGACCCATTC GACATATTTG GATCTGTGGA CCAAGGTGGC 
GTGCCCAGCA TGAAGCCATC AGGCTTTCCA CCTTTCGTTG GCTCCTTACC AACTTTTGTT 
GCTCAGCCAG CGGCTACCAC CAATCCTTCA CCAACTCATC CGGCAAGGGC ATCTGCAGAT 
CCATTTGCGA ACATCGCCGA CCTGGCGACA GGGCTCAACT TAAACTTTAA CCGCAGCACG 
CTAAGTGGAA AGTCTCCCGT CAACACTAGT CCGCAGCCTA CTCAGTTCTC AAGCCCCACT 
CACAAGCCAT CGCCATCCTC GCAACCACAA GCAACGTTTA TGCATACACC GCCGACACCG 
CAGACTCTGC CCTCGACATC GTCTATCAGG ACGCCGACGC AGCCACAGGC GGTACCTGCT 
CAATCAAGAC CAGACTACAG TCGACTTCAC TTTGATTCTC AGAAAGCAGC CCAGCAACCG 
GGCACTGGCG GGTCCAAGAA CTCTGACATA TTTGCTGACA TTCTGGGCCA ACAAGGATAC 
TCCTTTGGCA GCAAAATGAA CCAGTGTCCA CGATCCATCA ATGAAATGCG CAAGGAAGAT 
TTGTTCAAGG ATATGGACCC GAAGAAAGTG CGGATTATGG AATGGACTGA TGGTAAGAAA 
AACAACATCC GTGCGCTTCT CTGCTCTATG CACACAGTGT TGTGGGACAA TGCCAAGTGG 
CAGCGCTGCG AGATGTCCAC CATGGTGACA CCGACCGAGG TTAAGAAAGC ATACCGTCGC 
GCCTGCCTAG CAGTGCATCC TGATAAGCAT AATGGAACAG AGAATGAAGA GATTGCCAAG 
CTTATATTTA TGGAACTAAA CAACGCATGG ACAGATTTTG AAAACGATGC CACGCAGCAA 
AATATGTTTA ATGCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

aux ( NM_141181.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_649438.1 Drosophila melanogaster auxilin (aux), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa03788
Clone ID Related Accession (Same CDS sequence)	NM_164317.3 , NM_141181.3
Accession Version	NM_141181.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3498bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	auxilin, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_141181.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGCGAGT TCTTTAAGTC GCTCAACCTC AACTACTTCT CCTCTAGCGA GGTCAACGGA 
GCTGCAGGAA ACGGAGCACC AGAGGCCCTC GGTGGCAGGC TGGACAATGA CTTCGTGGGC 
CAGGTCGTTG AAGTCGCTGG ACACAGGCTG CGCATCAAGT GTGTCATCGC CGAAGGCGGA 
TACGCCTTCG TGTATGTAGC CCAAGATGTG CAAACTGGCA CAGAATACGC CCTCAAGCGT 
CTGATCGGGG CTGACATGCA AGCCTCCACC GCCATCATCA ACGAGATCAA CATCCACAAG 
CAGCTGTCGG GGCACGAGAA CATTGTCGCC TTTGTGGGTT CCAGTTATAC CGCCCCATCA 
ACTCAATTGG GAGCCCAGTA CTTGCTCCTC ACCGAGCTGT GTAAAGGCGG ATCTCTGGTG 
GACTGCTTCA GAACAAACAA TGCTCCATTC AATCCGACTT GTGTTCTGCG CATCTTCTAC 
CAAATGGCGC GGGCTGTGGC CAGCTTGCAC TCACAGTCAC CGCCGATAGC CCACCGAGAT 
ATAAAGATTG AGAACTTTCT CATTGGCAAC GACAAACAGA TCAAGCTGTG CGACTTTGGG 
TCCGCCAGCA CGGAGGTCCT GTCGCCCACG TTTGAGTGGA GCGCCAACCA GCGCAGCATG 
CTGGAGGACC AGCTAAACAC TGTTACTACT CCGATGTACC GATCCCCTGA GATGCTCGAC 
ACTTGGTCCA ACAACCCGAT TGGGCCCAAA GTGGACATAT GGGCTCTCGG ATGCATTCTC 
TACTTCCTGT GCTACCGCAA GCATCCCTAC GAGGATGGCG GAAAGCTGCG GATCATTAAC 
GCCAATTACA TGCTTCCGCC AGATCCTCAG TACCAGTGCT TCAGCGATAT AATCCGTGGC 
TGTTTAAAAG TGAACCCGTT CGAGCGCCTG GATATTACTA TGGTGCTGGA GGGCCTTGCA 
GCCCTCGCTG AGACACACAA TTGGTCGCTC AAAGGACCTC TAGACCTTCA TATAATGCCC 
ATCGAGTCCC TTCCGGCCGA AAGTCCTCTC AAAAGTTCTG CTTACTCTGA GGTTTACACC 
GAACCGCTTT CTGCCGCCAT ACCATCATCT TATAATGGAC ACGGCAGTCT TCTGTCGTCA 
CTGCGTGGTG GCGCTGGAAC GCTTTTAAAG AACATTAAAG ATACGTCCAC CAAAGTGATG 
CAAACAATGC AGCAATCTCT TGCTCGCAAC GATCTTGATA TAAGCCACAT TACCTCTCGC 
ATCCTGGTAA TGCCCTGCCC CTCTGACGGA TTCGAGTCGA CGTACAAGAC GAACAACATC 
GAAGACGTCC GACTATCTCT GGAGAGCCGA TTTGTCCCAC AAAAATTAAG CATTTACAAC 
TTTGGCCAAC GAACAGAGCC ACGGCTACCG CCACCCGTGC GAACAGTTGA GGCAGGTTCG 
GTTTATGGGT GCCCACAGGC ACATGCTCCT AATCTGCAGG GCCTTTTTAC CGTATCAGCC 
GACATGTACA ATTTTCTAAA TGCGGACCCC AAATCTGTCG TTATCGTGCA GACTGGAGAC 
TCGGGTGGAT GCACTGCTGC CACCGTTATA TGCGCGCTTC TTATGTATGC AGATTTGCTG 
CGGGAGCCGG AGGACGCGGT GCAAGTCTTC GCCGTTAAGC GGCATACCAT TAACCTCCGC 
CCCTCCGAGT TCCGTTACCT GTATTACTTC GGTGACATCC TGCGGCCCAC GCCCCTGCTG 
CCGCACTATA AGAACACAAC CCTTGTTTCA CTCAGCTGCC AGCCTGTGCC CAGAATGACC 
AAGGCACGCG ACGGCTGTAG GATCTATATG GAGGTTTATT GTAATGGAAA CTTGCTACTC 
AGCACTCTAC AGGACTATGA AAAAATGCGG TTGTATCAGG CCGGCCCCGG CAAAATAGTT 
CTGCCGATTA ACTTGACCGC ATGCGGTGAT GTGACAGTGG TATTATTTCA CGCCCGCAAA 
GGAATGGTGC GACCACAAGG CCTCAAGATA TGTCAGTTCC AATTCAACAC GGGATTCATC 
CCCGAACCAG AAACGTTGAT TACATTCACA AACCAGGACT TGGATGATCT GCCAGACCCT 
GAGCAGGTGA CACCTCGGTT CTGCGTTTCA CTTTCTCTGG CTGTTACTGA CTCGGAATCA 
CCTCCCAGCC ATAAGCCACC ATGGATGCCA GCCAAGCCCA AGCGGTCACC CGCCGCTTTG 
TTCAGCTCAG ATTTGGAGTA TGCAGAAATG CTCGACAATT TTGTAACCAA GCCTAGCACG 
CGTTCCTCAC CACCACCAGG TGCCCGCAAG AGTGATCGCG TCAGCTCTCC GCTCGTTTTG 
CCCGACGTCA CGGAGACCGC CCACGAACTG CCTTCGCCCA TGCCAGAGCC ATCTCCGCCC 
ATAGACTTGC TCAATTTAAA CCAGCAGCCC AGTGACGTAC CAGCAGCAGA TCCGTTAACC 
AGCGCTAAGC CCAGCACAGA TGCCAGCTTT GACTTGCTAG GGGCCTTCGG TGATGATGAC 
TCTACGGGCA TAGGATCTGC GCCAATTCCT GACATACTGC CACCACCGCC ACTGCAGCAG 
CCGCAACCAA AAATAAACAA TGACCCATTC GACATATTTG GATCTGTGGA CCAAGGTGGC 
GTGCCCAGCA TGAAGCCATC AGGCTTTCCA CCTTTCGTTG GCTCCTTACC AACTTTTGTT 
GCTCAGCCAG CGGCTACCAC CAATCCTTCA CCAACTCATC CGGCAAGGGC ATCTGCAGAT 
CCATTTGCGA ACATCGCCGA CCTGGCGACA GGGCTCAACT TAAACTTTAA CCGCAGCACG 
CTAAGTGGAA AGTCTCCCGT CAACACTAGT CCGCAGCCTA CTCAGTTCTC AAGCCCCACT 
CACAAGCCAT CGCCATCCTC GCAACCACAA GCAACGTTTA TGCATACACC GCCGACACCG 
CAGACTCTGC CCTCGACATC GTCTATCAGG ACGCCGACGC AGCCACAGGC GGTACCTGCT 
CAATCAAGAC CAGACTACAG TCGACTTCAC TTTGATTCTC AGAAAGCAGC CCAGCAACCG 
GGCACTGGCG GGTCCAAGAA CTCTGACATA TTTGCTGACA TTCTGGGCCA ACAAGGATAC 
TCCTTTGGCA GCAAAATGAA CCAGTGTCCA CGATCCATCA ATGAAATGCG CAAGGAAGAT 
TTGTTCAAGG ATATGGACCC GAAGAAAGTG CGGATTATGG AATGGACTGA TGGTAAGAAA 
AACAACATCC GTGCGCTTCT CTGCTCTATG CACACAGTGT TGTGGGACAA TGCCAAGTGG 
CAGCGCTGCG AGATGTCCAC CATGGTGACA CCGACCGAGG TTAAGAAAGC ATACCGTCGC 
GCCTGCCTAG CAGTGCATCC TGATAAGCAT AATGGAACAG AGAATGAAGA GATTGCCAAG 
CTTATATTTA TGGAACTAAA CAACGCATGG ACAGATTTTG AAAACGATGC CACGCAGCAA 
AATATGTTTA ATGCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_649438.1
CDS	158..3655
Translation	MGEFFKSLNLNYFSSSEVNGAAGNGAPEALGGRLDNDFVGQVVEVAGHRLRIKCVIAEGGYAFVYVAQDVQTGTEYALKRLIGADMQASTAIINEINIHKQLSGHENIVAFVGSSYTAPSTQLGAQYLLLTELCKGGSLVDCFRTNNAPFNPTCVLRIFYQMARAVASLHSQSPPIAHRDIKIENFLIGNDKQIKLCDFGSASTEVLSPTFEWSANQRSMLEDQLNTVTTPMYRSPEMLDTWSNNPIGPKVDIWALGCILYFLCYRKHPYEDGGKLRIINANYMLPPDPQYQCFSDIIRGCLKVNPFERLDITMVLEGLAALAETHNWSLKGPLDLHIMPIESLPAESPLKSSAYSEVYTEPLSAAIPSSYNGHGSLLSSLRGGAGTLLKNIKDTSTKVMQTMQQSLARNDLDISHITSRILVMPCPSDGFESTYKTNNIEDVRLSLESRFVPQKLSIYNFGQRTEPRLPPPVRTVEAGSVYGCPQAHAPNLQGLFTVSADMYNFLNADPKSVVIVQTGDSGGCTAATVICALLMYADLLREPEDAVQVFAVKRHTINLRPSEFRYLYYFGDILRPTPLLPHYKNTTLVSLSCQPVPRMTKARDGCRIYMEVYCNGNLLLSTLQDYEKMRLYQAGPGKIVLPINLTACGDVTVVLFHARKGMVRPQGLKICQFQFNTGFIPEPETLITFTNQDLDDLPDPEQVTPRFCVSLSLAVTDSESPPSHKPPWMPAKPKRSPAALFSSDLEYAEMLDNFVTKPSTRSSPPPGARKSDRVSSPLVLPDVTETAHELPSPMPEPSPPIDLLNLNQQPSDVPAADPLTSAKPSTDASFDLLGAFGDDDSTGIGSAPIPDILPPPPLQQPQPKINNDPFDIFGSVDQGGVPSMKPSGFPPFVGSLPTFVAQPAATTNPSPTHPARASADPFANIADLATGLNLNFNRSTLSGKSPVNTSPQPTQFSSPTHKPSPSSQPQATFMHTPPTPQTLPSTSSIRTPTQPQAVPAQSRPDYSRLHFDSQKAAQQPGTGGSKNSDIFADILGQQGYSFGSKMNQCPRSINEMRKEDLFKDMDPKKVRIMEWTDGKKNNIRALLCSMHTVLWDNAKWQRCEMSTMVTPTEVKKAYRRACLAVHPDKHNGTENEEIAKLIFMELNNAWTDFENDATQQNMFNA

Target ORF information:

RefSeq Version	NM_141181.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster auxilin (aux), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_141181.3

ORF Insert Sequence:

ATGGGCGAGT TCTTTAAGTC GCTCAACCTC AACTACTTCT CCTCTAGCGA GGTCAACGGA 
GCTGCAGGAA ACGGAGCACC AGAGGCCCTC GGTGGCAGGC TGGACAATGA CTTCGTGGGC 
CAGGTCGTTG AAGTCGCTGG ACACAGGCTG CGCATCAAGT GTGTCATCGC CGAAGGCGGA 
TACGCCTTCG TGTATGTAGC CCAAGATGTG CAAACTGGCA CAGAATACGC CCTCAAGCGT 
CTGATCGGGG CTGACATGCA AGCCTCCACC GCCATCATCA ACGAGATCAA CATCCACAAG 
CAGCTGTCGG GGCACGAGAA CATTGTCGCC TTTGTGGGTT CCAGTTATAC CGCCCCATCA 
ACTCAATTGG GAGCCCAGTA CTTGCTCCTC ACCGAGCTGT GTAAAGGCGG ATCTCTGGTG 
GACTGCTTCA GAACAAACAA TGCTCCATTC AATCCGACTT GTGTTCTGCG CATCTTCTAC 
CAAATGGCGC GGGCTGTGGC CAGCTTGCAC TCACAGTCAC CGCCGATAGC CCACCGAGAT 
ATAAAGATTG AGAACTTTCT CATTGGCAAC GACAAACAGA TCAAGCTGTG CGACTTTGGG 
TCCGCCAGCA CGGAGGTCCT GTCGCCCACG TTTGAGTGGA GCGCCAACCA GCGCAGCATG 
CTGGAGGACC AGCTAAACAC TGTTACTACT CCGATGTACC GATCCCCTGA GATGCTCGAC 
ACTTGGTCCA ACAACCCGAT TGGGCCCAAA GTGGACATAT GGGCTCTCGG ATGCATTCTC 
TACTTCCTGT GCTACCGCAA GCATCCCTAC GAGGATGGCG GAAAGCTGCG GATCATTAAC 
GCCAATTACA TGCTTCCGCC AGATCCTCAG TACCAGTGCT TCAGCGATAT AATCCGTGGC 
TGTTTAAAAG TGAACCCGTT CGAGCGCCTG GATATTACTA TGGTGCTGGA GGGCCTTGCA 
GCCCTCGCTG AGACACACAA TTGGTCGCTC AAAGGACCTC TAGACCTTCA TATAATGCCC 
ATCGAGTCCC TTCCGGCCGA AAGTCCTCTC AAAAGTTCTG CTTACTCTGA GGTTTACACC 
GAACCGCTTT CTGCCGCCAT ACCATCATCT TATAATGGAC ACGGCAGTCT TCTGTCGTCA 
CTGCGTGGTG GCGCTGGAAC GCTTTTAAAG AACATTAAAG ATACGTCCAC CAAAGTGATG 
CAAACAATGC AGCAATCTCT TGCTCGCAAC GATCTTGATA TAAGCCACAT TACCTCTCGC 
ATCCTGGTAA TGCCCTGCCC CTCTGACGGA TTCGAGTCGA CGTACAAGAC GAACAACATC 
GAAGACGTCC GACTATCTCT GGAGAGCCGA TTTGTCCCAC AAAAATTAAG CATTTACAAC 
TTTGGCCAAC GAACAGAGCC ACGGCTACCG CCACCCGTGC GAACAGTTGA GGCAGGTTCG 
GTTTATGGGT GCCCACAGGC ACATGCTCCT AATCTGCAGG GCCTTTTTAC CGTATCAGCC 
GACATGTACA ATTTTCTAAA TGCGGACCCC AAATCTGTCG TTATCGTGCA GACTGGAGAC 
TCGGGTGGAT GCACTGCTGC CACCGTTATA TGCGCGCTTC TTATGTATGC AGATTTGCTG 
CGGGAGCCGG AGGACGCGGT GCAAGTCTTC GCCGTTAAGC GGCATACCAT TAACCTCCGC 
CCCTCCGAGT TCCGTTACCT GTATTACTTC GGTGACATCC TGCGGCCCAC GCCCCTGCTG 
CCGCACTATA AGAACACAAC CCTTGTTTCA CTCAGCTGCC AGCCTGTGCC CAGAATGACC 
AAGGCACGCG ACGGCTGTAG GATCTATATG GAGGTTTATT GTAATGGAAA CTTGCTACTC 
AGCACTCTAC AGGACTATGA AAAAATGCGG TTGTATCAGG CCGGCCCCGG CAAAATAGTT 
CTGCCGATTA ACTTGACCGC ATGCGGTGAT GTGACAGTGG TATTATTTCA CGCCCGCAAA 
GGAATGGTGC GACCACAAGG CCTCAAGATA TGTCAGTTCC AATTCAACAC GGGATTCATC 
CCCGAACCAG AAACGTTGAT TACATTCACA AACCAGGACT TGGATGATCT GCCAGACCCT 
GAGCAGGTGA CACCTCGGTT CTGCGTTTCA CTTTCTCTGG CTGTTACTGA CTCGGAATCA 
CCTCCCAGCC ATAAGCCACC ATGGATGCCA GCCAAGCCCA AGCGGTCACC CGCCGCTTTG 
TTCAGCTCAG ATTTGGAGTA TGCAGAAATG CTCGACAATT TTGTAACCAA GCCTAGCACG 
CGTTCCTCAC CACCACCAGG TGCCCGCAAG AGTGATCGCG TCAGCTCTCC GCTCGTTTTG 
CCCGACGTCA CGGAGACCGC CCACGAACTG CCTTCGCCCA TGCCAGAGCC ATCTCCGCCC 
ATAGACTTGC TCAATTTAAA CCAGCAGCCC AGTGACGTAC CAGCAGCAGA TCCGTTAACC 
AGCGCTAAGC CCAGCACAGA TGCCAGCTTT GACTTGCTAG GGGCCTTCGG TGATGATGAC 
TCTACGGGCA TAGGATCTGC GCCAATTCCT GACATACTGC CACCACCGCC ACTGCAGCAG 
CCGCAACCAA AAATAAACAA TGACCCATTC GACATATTTG GATCTGTGGA CCAAGGTGGC 
GTGCCCAGCA TGAAGCCATC AGGCTTTCCA CCTTTCGTTG GCTCCTTACC AACTTTTGTT 
GCTCAGCCAG CGGCTACCAC CAATCCTTCA CCAACTCATC CGGCAAGGGC ATCTGCAGAT 
CCATTTGCGA ACATCGCCGA CCTGGCGACA GGGCTCAACT TAAACTTTAA CCGCAGCACG 
CTAAGTGGAA AGTCTCCCGT CAACACTAGT CCGCAGCCTA CTCAGTTCTC AAGCCCCACT 
CACAAGCCAT CGCCATCCTC GCAACCACAA GCAACGTTTA TGCATACACC GCCGACACCG 
CAGACTCTGC CCTCGACATC GTCTATCAGG ACGCCGACGC AGCCACAGGC GGTACCTGCT 
CAATCAAGAC CAGACTACAG TCGACTTCAC TTTGATTCTC AGAAAGCAGC CCAGCAACCG 
GGCACTGGCG GGTCCAAGAA CTCTGACATA TTTGCTGACA TTCTGGGCCA ACAAGGATAC 
TCCTTTGGCA GCAAAATGAA CCAGTGTCCA CGATCCATCA ATGAAATGCG CAAGGAAGAT 
TTGTTCAAGG ATATGGACCC GAAGAAAGTG CGGATTATGG AATGGACTGA TGGTAAGAAA 
AACAACATCC GTGCGCTTCT CTGCTCTATG CACACAGTGT TGTGGGACAA TGCCAAGTGG 
CAGCGCTGCG AGATGTCCAC CATGGTGACA CCGACCGAGG TTAAGAAAGC ATACCGTCGC 
GCCTGCCTAG CAGTGCATCC TGATAAGCAT AATGGAACAG AGAATGAAGA GATTGCCAAG 
CTTATATTTA TGGAACTAAA CAACGCATGG ACAGATTTTG AAAACGATGC CACGCAGCAA 
AATATGTTTA ATGCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

aux ( NM_001275314.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262243.1 Drosophila melanogaster auxilin (aux), transcript variant C, mRNA.

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CloneID	OFa07849
Clone ID Related Accession (Same CDS sequence)	NM_001275314.1
Accession Version	NM_001275314.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3462bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	auxilin, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGCGAGT TCTTTAAGTC GCTCAACCTC AACTACTTCT CCTCTAGCGA GGTCAACGGA 
GCTGCAGGAA ACGGAGCACC AGAGGCCCTC GGTGGCAGGC TGGACAATGA CTTCGTGGGC 
CAGGTCGTTG AAGTCGCTGG ACACAGGCTG CGCATCAAGT GTGTCATCGC CGAAGGCGGA 
TACGCCTTCG TGTATGTAGC CCAAGATGTG CAAACTGGCA CAGAATACGC CCTCAAGCGT 
CTGATCGGGG CTGACATGCA AGCCTCCACC GCCATCATCA ACGAGATCAA CATCCACAAG 
CAGCTGTCGG GGCACGAGAA CATTGTCGCC TTTGTGGGTT CCAGTTATAC CGCCCCATCA 
ACTCAATTGG GAGCCCAGTA CTTGCTCCTC ACCGAGCTGT GTAAAGGCGG ATCTCTGGTG 
GACTGCTTCA GAACAAACAA TGCTCCATTC AATCCGACTT GTGTTCTGCG CATCTTCTAC 
CAAATGGCGC GGGCTGTGGC CAGCTTGCAC TCACAGTCAC CGCCGATAGC CCACCGAGAT 
ATAAAGATTG AGAACTTTCT CATTGGCAAC GACAAACAGA TCAAGCTGTG CGACTTTGGG 
TCCGCCAGCA CGGAGGTCCT GTCGCCCACG TTTGAGTGGA GCGCCAACCA GCGCAGCATG 
CTGGAGGACC AGCTAAACAC TGTTACTACT CCGATGTACC GATCCCCTGA GATGCTCGAC 
ACTTGGTCCA ACAACCCGAT TGGGCCCAAA GTGGACATAT GGGCTCTCGG ATGCATTCTC 
TACTTCCTGT GCTACCGCAA GCATCCCTAC GAGGATGGCG GAAAGCTGCG GATCATTAAC 
GCCAATTACA TGCTTCCGCC AGATCCTCAG TACCAGTGCT TCAGCGATAT AATCCGTGGC 
TGTTTAAAAG TGAACCCGTT CGAGCGCCTG GATATTACTA TGGTGCTGGA GGGCCTTGCA 
GCCCTCGCTG AGACACACAA TTGGTCGCTC AAAGGACCTC TAGACCTTCA TATAATGCCC 
ATCGAGTCCC TTCCGGCCGA AAGTCCTCTC AAAAGTTCTG CTTACTCTGA GGTTTACACC 
GAACCGCTTT CTGCCGCCAT ACCATCATCT TATAATGGAC ACGGCAGTCT TCTGTCGTCA 
CTGCGTGGTG GCGCTGGAAC GCTTTTAAAG AACATTAAAG ATACGTCCAC CAAAGTGATG 
CAAACAATGC AGCAATCTCT TGCTCGCAAC GATCTTGATA TAAGCCACAT TACCTCTCGC 
ATCCTGGTAA TGCCCTGCCC CTCTGACGGA TTCGAGTCGA CGTACAAGAC GAACAACATC 
GAAGACGTCC GACTATCTCT GGAGAGCCGA TTTGTCCCAC AAAAATTAAG CATTTACAAC 
TTTGGCCAAC GAACAGAGCC ACGGCTACCG CCACCCGTGC GAACAGTTGA GGCAGGTTCG 
GTTTATGGGT GCCCACAGGC ACATGCTCCT AATCTGCAGG GCCTTTTTAC CGTATCAGCC 
GACATGTACA ATTTTCTAAA TGCGGACCCC AAATCTGTCG TTATCGTGCA GACTGGAGAC 
TCGGGTGGAT GCACTGCTGC CACCGTTATA TGCGCGCTTC TTATGTATGC AGATTTGCTG 
CGGGAGCCGG AGGACGCGGT GCAAGTCTTC GCCGTTAAGC GGCATACCAT TAACCTCCGC 
CCCTCCGAGT TCCGTTACCT GTATTACTTC GGTGACATCC TGCGGCCCAC GCCCCTGCTG 
CCGCACTATA AGAACACAAC CCTTGTTTCA CTCAGCTGCC AGCCTGTGCC CAGAATGACC 
AAGGCACGCG ACGGCTGTAG GATCTATATG GAGGTTTATT GTAATGGAAA CTTGCTACTC 
AGCACTCTAC AGGACTATGA AAAAATGCGG TTGTATCAGG CCGGCCCCGG CAAAATAGTT 
CTGCCGATTA ACTTGACCGC ATGCGGTGAT GTGACAGTGG TATTATTTCA CGCCCGCAAA 
GGAATGGTGC GACCACAAGG CCTCAAGATA TGTCAGTTCC AATTCAACAC GGGATTCATC 
CCCGAACCAG AAACGTTGAT TACATTCACA AACCAGGACT TGGATGATCT GCCAGACCCT 
GAGCAGGTGA CACCTCGGTT CTGCGTTTCA CTTTCTCTGG CTGTTACTGA CTCGGAATCA 
CCTCCCAGCC ATAAGCCACC ATGGATGCCA GCCAAGCCCA AGCGGTCACC CGCCGCTTTG 
TTCAGCTCAG ATTTGGAGTA TGCAGAAATG CTCGACAATT TTGGTGCCCG CAAGAGTGAT 
CGCGTCAGCT CTCCGCTCGT TTTGCCCGAC GTCACGGAGA CCGCCCACGA ACTGCCTTCG 
CCCATGCCAG AGCCATCTCC GCCCATAGAC TTGCTCAATT TAAACCAGCA GCCCAGTGAC 
GTACCAGCAG CAGATCCGTT AACCAGCGCT AAGCCCAGCA CAGATGCCAG CTTTGACTTG 
CTAGGGGCCT TCGGTGATGA TGACTCTACG GGCATAGGAT CTGCGCCAAT TCCTGACATA 
CTGCCACCAC CGCCACTGCA GCAGCCGCAA CCAAAAATAA ACAATGACCC ATTCGACATA 
TTTGGATCTG TGGACCAAGG TGGCGTGCCC AGCATGAAGC CATCAGGCTT TCCACCTTTC 
GTTGGCTCCT TACCAACTTT TGTTGCTCAG CCAGCGGCTA CCACCAATCC TTCACCAACT 
CATCCGGCAA GGGCATCTGC AGATCCATTT GCGAACATCG CCGACCTGGC GACAGGGCTC 
AACTTAAACT TTAACCGCAG CACGCTAAGT GGAAAGTCTC CCGTCAACAC TAGTCCGCAG 
CCTACTCAGT TCTCAAGCCC CACTCACAAG CCATCGCCAT CCTCGCAACC ACAAGCAACG 
TTTATGCATA CACCGCCGAC ACCGCAGACT CTGCCCTCGA CATCGTCTAT CAGGACGCCG 
ACGCAGCCAC AGGCGGTACC TGCTCAATCA AGACCAGACT ACAGTCGACT TCACTTTGAT 
TCTCAGAAAG CAGCCCAGCA ACCGGGCACT GGCGGGTCCA AGAACTCTGA CATATTTGCT 
GACATTCTGG GCCAACAAGG ATACTCCTTT GGCAGCAAAA TGAACCAGTG TCCACGATCC 
ATCAATGAAA TGCGCAAGGA AGATTTGTTC AAGGATATGG ACCCGAAGAA AGTGCGGATT 
ATGGAATGGA CTGATGGTAA GAAAAACAAC ATCCGTGCGC TTCTCTGCTC TATGCACACA 
GTGTTGTGGG ACAATGCCAA GTGGCAGCGC TGCGAGATGT CCACCATGGT GACACCGACC 
GAGGTTAAGA AAGCATACCG TCGCGCCTGC CTAGCAGTGC ATCCTGATAA GCATAATGGA 
ACAGAGAATG AAGAGATTGC CAAGCTTATA TTTATGGAAC TAAACAACGC ATGGACAGAT 
TTTGAAAACG ATGCCACGCA GCAAAATATG TTTAATGCGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262243.1
CDS	158..3619
Translation	MGEFFKSLNLNYFSSSEVNGAAGNGAPEALGGRLDNDFVGQVVEVAGHRLRIKCVIAEGGYAFVYVAQDVQTGTEYALKRLIGADMQASTAIINEINIHKQLSGHENIVAFVGSSYTAPSTQLGAQYLLLTELCKGGSLVDCFRTNNAPFNPTCVLRIFYQMARAVASLHSQSPPIAHRDIKIENFLIGNDKQIKLCDFGSASTEVLSPTFEWSANQRSMLEDQLNTVTTPMYRSPEMLDTWSNNPIGPKVDIWALGCILYFLCYRKHPYEDGGKLRIINANYMLPPDPQYQCFSDIIRGCLKVNPFERLDITMVLEGLAALAETHNWSLKGPLDLHIMPIESLPAESPLKSSAYSEVYTEPLSAAIPSSYNGHGSLLSSLRGGAGTLLKNIKDTSTKVMQTMQQSLARNDLDISHITSRILVMPCPSDGFESTYKTNNIEDVRLSLESRFVPQKLSIYNFGQRTEPRLPPPVRTVEAGSVYGCPQAHAPNLQGLFTVSADMYNFLNADPKSVVIVQTGDSGGCTAATVICALLMYADLLREPEDAVQVFAVKRHTINLRPSEFRYLYYFGDILRPTPLLPHYKNTTLVSLSCQPVPRMTKARDGCRIYMEVYCNGNLLLSTLQDYEKMRLYQAGPGKIVLPINLTACGDVTVVLFHARKGMVRPQGLKICQFQFNTGFIPEPETLITFTNQDLDDLPDPEQVTPRFCVSLSLAVTDSESPPSHKPPWMPAKPKRSPAALFSSDLEYAEMLDNFGARKSDRVSSPLVLPDVTETAHELPSPMPEPSPPIDLLNLNQQPSDVPAADPLTSAKPSTDASFDLLGAFGDDDSTGIGSAPIPDILPPPPLQQPQPKINNDPFDIFGSVDQGGVPSMKPSGFPPFVGSLPTFVAQPAATTNPSPTHPARASADPFANIADLATGLNLNFNRSTLSGKSPVNTSPQPTQFSSPTHKPSPSSQPQATFMHTPPTPQTLPSTSSIRTPTQPQAVPAQSRPDYSRLHFDSQKAAQQPGTGGSKNSDIFADILGQQGYSFGSKMNQCPRSINEMRKEDLFKDMDPKKVRIMEWTDGKKNNIRALLCSMHTVLWDNAKWQRCEMSTMVTPTEVKKAYRRACLAVHPDKHNGTENEEIAKLIFMELNNAWTDFENDATQQNMFNA

Target ORF information:

RefSeq Version	NM_001275314.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster auxilin (aux), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275314.1

ORF Insert Sequence:

ATGGGCGAGT TCTTTAAGTC GCTCAACCTC AACTACTTCT CCTCTAGCGA GGTCAACGGA 
GCTGCAGGAA ACGGAGCACC AGAGGCCCTC GGTGGCAGGC TGGACAATGA CTTCGTGGGC 
CAGGTCGTTG AAGTCGCTGG ACACAGGCTG CGCATCAAGT GTGTCATCGC CGAAGGCGGA 
TACGCCTTCG TGTATGTAGC CCAAGATGTG CAAACTGGCA CAGAATACGC CCTCAAGCGT 
CTGATCGGGG CTGACATGCA AGCCTCCACC GCCATCATCA ACGAGATCAA CATCCACAAG 
CAGCTGTCGG GGCACGAGAA CATTGTCGCC TTTGTGGGTT CCAGTTATAC CGCCCCATCA 
ACTCAATTGG GAGCCCAGTA CTTGCTCCTC ACCGAGCTGT GTAAAGGCGG ATCTCTGGTG 
GACTGCTTCA GAACAAACAA TGCTCCATTC AATCCGACTT GTGTTCTGCG CATCTTCTAC 
CAAATGGCGC GGGCTGTGGC CAGCTTGCAC TCACAGTCAC CGCCGATAGC CCACCGAGAT 
ATAAAGATTG AGAACTTTCT CATTGGCAAC GACAAACAGA TCAAGCTGTG CGACTTTGGG 
TCCGCCAGCA CGGAGGTCCT GTCGCCCACG TTTGAGTGGA GCGCCAACCA GCGCAGCATG 
CTGGAGGACC AGCTAAACAC TGTTACTACT CCGATGTACC GATCCCCTGA GATGCTCGAC 
ACTTGGTCCA ACAACCCGAT TGGGCCCAAA GTGGACATAT GGGCTCTCGG ATGCATTCTC 
TACTTCCTGT GCTACCGCAA GCATCCCTAC GAGGATGGCG GAAAGCTGCG GATCATTAAC 
GCCAATTACA TGCTTCCGCC AGATCCTCAG TACCAGTGCT TCAGCGATAT AATCCGTGGC 
TGTTTAAAAG TGAACCCGTT CGAGCGCCTG GATATTACTA TGGTGCTGGA GGGCCTTGCA 
GCCCTCGCTG AGACACACAA TTGGTCGCTC AAAGGACCTC TAGACCTTCA TATAATGCCC 
ATCGAGTCCC TTCCGGCCGA AAGTCCTCTC AAAAGTTCTG CTTACTCTGA GGTTTACACC 
GAACCGCTTT CTGCCGCCAT ACCATCATCT TATAATGGAC ACGGCAGTCT TCTGTCGTCA 
CTGCGTGGTG GCGCTGGAAC GCTTTTAAAG AACATTAAAG ATACGTCCAC CAAAGTGATG 
CAAACAATGC AGCAATCTCT TGCTCGCAAC GATCTTGATA TAAGCCACAT TACCTCTCGC 
ATCCTGGTAA TGCCCTGCCC CTCTGACGGA TTCGAGTCGA CGTACAAGAC GAACAACATC 
GAAGACGTCC GACTATCTCT GGAGAGCCGA TTTGTCCCAC AAAAATTAAG CATTTACAAC 
TTTGGCCAAC GAACAGAGCC ACGGCTACCG CCACCCGTGC GAACAGTTGA GGCAGGTTCG 
GTTTATGGGT GCCCACAGGC ACATGCTCCT AATCTGCAGG GCCTTTTTAC CGTATCAGCC 
GACATGTACA ATTTTCTAAA TGCGGACCCC AAATCTGTCG TTATCGTGCA GACTGGAGAC 
TCGGGTGGAT GCACTGCTGC CACCGTTATA TGCGCGCTTC TTATGTATGC AGATTTGCTG 
CGGGAGCCGG AGGACGCGGT GCAAGTCTTC GCCGTTAAGC GGCATACCAT TAACCTCCGC 
CCCTCCGAGT TCCGTTACCT GTATTACTTC GGTGACATCC TGCGGCCCAC GCCCCTGCTG 
CCGCACTATA AGAACACAAC CCTTGTTTCA CTCAGCTGCC AGCCTGTGCC CAGAATGACC 
AAGGCACGCG ACGGCTGTAG GATCTATATG GAGGTTTATT GTAATGGAAA CTTGCTACTC 
AGCACTCTAC AGGACTATGA AAAAATGCGG TTGTATCAGG CCGGCCCCGG CAAAATAGTT 
CTGCCGATTA ACTTGACCGC ATGCGGTGAT GTGACAGTGG TATTATTTCA CGCCCGCAAA 
GGAATGGTGC GACCACAAGG CCTCAAGATA TGTCAGTTCC AATTCAACAC GGGATTCATC 
CCCGAACCAG AAACGTTGAT TACATTCACA AACCAGGACT TGGATGATCT GCCAGACCCT 
GAGCAGGTGA CACCTCGGTT CTGCGTTTCA CTTTCTCTGG CTGTTACTGA CTCGGAATCA 
CCTCCCAGCC ATAAGCCACC ATGGATGCCA GCCAAGCCCA AGCGGTCACC CGCCGCTTTG 
TTCAGCTCAG ATTTGGAGTA TGCAGAAATG CTCGACAATT TTGGTGCCCG CAAGAGTGAT 
CGCGTCAGCT CTCCGCTCGT TTTGCCCGAC GTCACGGAGA CCGCCCACGA ACTGCCTTCG 
CCCATGCCAG AGCCATCTCC GCCCATAGAC TTGCTCAATT TAAACCAGCA GCCCAGTGAC 
GTACCAGCAG CAGATCCGTT AACCAGCGCT AAGCCCAGCA CAGATGCCAG CTTTGACTTG 
CTAGGGGCCT TCGGTGATGA TGACTCTACG GGCATAGGAT CTGCGCCAAT TCCTGACATA 
CTGCCACCAC CGCCACTGCA GCAGCCGCAA CCAAAAATAA ACAATGACCC ATTCGACATA 
TTTGGATCTG TGGACCAAGG TGGCGTGCCC AGCATGAAGC CATCAGGCTT TCCACCTTTC 
GTTGGCTCCT TACCAACTTT TGTTGCTCAG CCAGCGGCTA CCACCAATCC TTCACCAACT 
CATCCGGCAA GGGCATCTGC AGATCCATTT GCGAACATCG CCGACCTGGC GACAGGGCTC 
AACTTAAACT TTAACCGCAG CACGCTAAGT GGAAAGTCTC CCGTCAACAC TAGTCCGCAG 
CCTACTCAGT TCTCAAGCCC CACTCACAAG CCATCGCCAT CCTCGCAACC ACAAGCAACG 
TTTATGCATA CACCGCCGAC ACCGCAGACT CTGCCCTCGA CATCGTCTAT CAGGACGCCG 
ACGCAGCCAC AGGCGGTACC TGCTCAATCA AGACCAGACT ACAGTCGACT TCACTTTGAT 
TCTCAGAAAG CAGCCCAGCA ACCGGGCACT GGCGGGTCCA AGAACTCTGA CATATTTGCT 
GACATTCTGG GCCAACAAGG ATACTCCTTT GGCAGCAAAA TGAACCAGTG TCCACGATCC 
ATCAATGAAA TGCGCAAGGA AGATTTGTTC AAGGATATGG ACCCGAAGAA AGTGCGGATT 
ATGGAATGGA CTGATGGTAA GAAAAACAAC ATCCGTGCGC TTCTCTGCTC TATGCACACA 
GTGTTGTGGG ACAATGCCAA GTGGCAGCGC TGCGAGATGT CCACCATGGT GACACCGACC 
GAGGTTAAGA AAGCATACCG TCGCGCCTGC CTAGCAGTGC ATCCTGATAA GCATAATGGA 
ACAGAGAATG AAGAGATTGC CAAGCTTATA TTTATGGAAC TAAACAACGC ATGGACAGAT 
TTTGAAAACG ATGCCACGCA GCAAAATATG TTTAATGCGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

aux ( NM_001275315.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262244.1 Drosophila melanogaster auxilin (aux), transcript variant D, mRNA.

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CloneID	OFa07850
Clone ID Related Accession (Same CDS sequence)	NM_001275315.1
Accession Version	NM_001275315.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3474bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	auxilin, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGCGAGT TCTTTAAGTC GCTCAACCTC AACTACTTCT CCTCTAGCGA GGTCAACGGA 
GCTGCAGGAA ACGGAGCACC AGAGGCCCTC GGTGGCAGGC TGGACAATGA CTTCGTGGGC 
CAGGTCGTTG AAGTCGCTGG ACACAGGCTG CGCATCAAGT GTGTCATCGC CGAAGCCCAA 
GATGTGCAAA CTGGCACAGA ATACGCCCTC AAGCGTCTGA TCGGGGCTGA CATGCAAGCC 
TCCACCGCCA TCATCAACGA GATCAACATC CACAAGCAGC TGTCGGGGCA CGAGAACATT 
GTCGCCTTTG TGGGTTCCAG TTATACCGCC CCATCAACTC AATTGGGAGC CCAGTACTTG 
CTCCTCACCG AGCTGTGTAA AGGCGGATCT CTGGTGGACT GCTTCAGAAC AAACAATGCT 
CCATTCAATC CGACTTGTGT TCTGCGCATC TTCTACCAAA TGGCGCGGGC TGTGGCCAGC 
TTGCACTCAC AGTCACCGCC GATAGCCCAC CGAGATATAA AGATTGAGAA CTTTCTCATT 
GGCAACGACA AACAGATCAA GCTGTGCGAC TTTGGGTCCG CCAGCACGGA GGTCCTGTCG 
CCCACGTTTG AGTGGAGCGC CAACCAGCGC AGCATGCTGG AGGACCAGCT AAACACTGTT 
ACTACTCCGA TGTACCGATC CCCTGAGATG CTCGACACTT GGTCCAACAA CCCGATTGGG 
CCCAAAGTGG ACATATGGGC TCTCGGATGC ATTCTCTACT TCCTGTGCTA CCGCAAGCAT 
CCCTACGAGG ATGGCGGAAA GCTGCGGATC ATTAACGCCA ATTACATGCT TCCGCCAGAT 
CCTCAGTACC AGTGCTTCAG CGATATAATC CGTGGCTGTT TAAAAGTGAA CCCGTTCGAG 
CGCCTGGATA TTACTATGGT GCTGGAGGGC CTTGCAGCCC TCGCTGAGAC ACACAATTGG 
TCGCTCAAAG GACCTCTAGA CCTTCATATA ATGCCCATCG AGTCCCTTCC GGCCGAAAGT 
CCTCTCAAAA GTTCTGCTTA CTCTGAGGTT TACACCGAAC CGCTTTCTGC CGCCATACCA 
TCATCTTATA ATGGACACGG CAGTCTTCTG TCGTCACTGC GTGGTGGCGC TGGAACGCTT 
TTAAAGAACA TTAAAGATAC GTCCACCAAA GTGATGCAAA CAATGCAGCA ATCTCTTGCT 
CGCAACGATC TTGATATAAG CCACATTACC TCTCGCATCC TGGTAATGCC CTGCCCCTCT 
GACGGATTCG AGTCGACGTA CAAGACGAAC AACATCGAAG ACGTCCGACT ATCTCTGGAG 
AGCCGATTTG TCCCACAAAA ATTAAGCATT TACAACTTTG GCCAACGAAC AGAGCCACGG 
CTACCGCCAC CCGTGCGAAC AGTTGAGGCA GGTTCGGTTT ATGGGTGCCC ACAGGCACAT 
GCTCCTAATC TGCAGGGCCT TTTTACCGTA TCAGCCGACA TGTACAATTT TCTAAATGCG 
GACCCCAAAT CTGTCGTTAT CGTGCAGACT GGAGACTCGG GTGGATGCAC TGCTGCCACC 
GTTATATGCG CGCTTCTTAT GTATGCAGAT TTGCTGCGGG AGCCGGAGGA CGCGGTGCAA 
GTCTTCGCCG TTAAGCGGCA TACCATTAAC CTCCGCCCCT CCGAGTTCCG TTACCTGTAT 
TACTTCGGTG ACATCCTGCG GCCCACGCCC CTGCTGCCGC ACTATAAGAA CACAACCCTT 
GTTTCACTCA GCTGCCAGCC TGTGCCCAGA ATGACCAAGG CACGCGACGG CTGTAGGATC 
TATATGGAGG TTTATTGTAA TGGAAACTTG CTACTCAGCA CTCTACAGGA CTATGAAAAA 
ATGCGGTTGT ATCAGGCCGG CCCCGGCAAA ATAGTTCTGC CGATTAACTT GACCGCATGC 
GGTGATGTGA CAGTGGTATT ATTTCACGCC CGCAAAGGAA TGGTGCGACC ACAAGGCCTC 
AAGATATGTC AGTTCCAATT CAACACGGGA TTCATCCCCG AACCAGAAAC GTTGATTACA 
TTCACAAACC AGGACTTGGA TGATCTGCCA GACCCTGAGC AGGTGACACC TCGGTTCTGC 
GTTTCACTTT CTCTGGCTGT TACTGACTCG GAATCACCTC CCAGCCATAA GCCACCATGG 
ATGCCAGCCA AGCCCAAGCG GTCACCCGCC GCTTTGTTCA GCTCAGATTT GGAGTATGCA 
GAAATGCTCG ACAATTTTGT AACCAAGCCT AGCACGCGTT CCTCACCACC ACCAGGTGCC 
CGCAAGAGTG ATCGCGTCAG CTCTCCGCTC GTTTTGCCCG ACGTCACGGA GACCGCCCAC 
GAACTGCCTT CGCCCATGCC AGAGCCATCT CCGCCCATAG ACTTGCTCAA TTTAAACCAG 
CAGCCCAGTG ACGTACCAGC AGCAGATCCG TTAACCAGCG CTAAGCCCAG CACAGATGCC 
AGCTTTGACT TGCTAGGGGC CTTCGGTGAT GATGACTCTA CGGGCATAGG ATCTGCGCCA 
ATTCCTGACA TACTGCCACC ACCGCCACTG CAGCAGCCGC AACCAAAAAT AAACAATGAC 
CCATTCGACA TATTTGGATC TGTGGACCAA GGTGGCGTGC CCAGCATGAA GCCATCAGGC 
TTTCCACCTT TCGTTGGCTC CTTACCAACT TTTGTTGCTC AGCCAGCGGC TACCACCAAT 
CCTTCACCAA CTCATCCGGC AAGGGCATCT GCAGATCCAT TTGCGAACAT CGCCGACCTG 
GCGACAGGGC TCAACTTAAA CTTTAACCGC AGCACGCTAA GTGGAAAGTC TCCCGTCAAC 
ACTAGTCCGC AGCCTACTCA GTTCTCAAGC CCCACTCACA AGCCATCGCC ATCCTCGCAA 
CCACAAGCAA CGTTTATGCA TACACCGCCG ACACCGCAGA CTCTGCCCTC GACATCGTCT 
ATCAGGACGC CGACGCAGCC ACAGGCGGTA CCTGCTCAAT CAAGACCAGA CTACAGTCGA 
CTTCACTTTG ATTCTCAGAA AGCAGCCCAG CAACCGGGCA CTGGCGGGTC CAAGAACTCT 
GACATATTTG CTGACATTCT GGGCCAACAA GGATACTCCT TTGGCAGCAA AATGAACCAG 
TGTCCACGAT CCATCAATGA AATGCGCAAG GAAGATTTGT TCAAGGATAT GGACCCGAAG 
AAAGTGCGGA TTATGGAATG GACTGATGGT AAGAAAAACA ACATCCGTGC GCTTCTCTGC 
TCTATGCACA CAGTGTTGTG GGACAATGCC AAGTGGCAGC GCTGCGAGAT GTCCACCATG 
GTGACACCGA CCGAGGTTAA GAAAGCATAC CGTCGCGCCT GCCTAGCAGT GCATCCTGAT 
AAGCATAATG GAACAGAGAA TGAAGAGATT GCCAAGCTTA TATTTATGGA ACTAAACAAC 
GCATGGACAG ATTTTGAAAA CGATGCCACG CAGCAAAATA TGTTTAATGC GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262244.1
CDS	158..3631
Translation	MGEFFKSLNLNYFSSSEVNGAAGNGAPEALGGRLDNDFVGQVVEVAGHRLRIKCVIAEAQDVQTGTEYALKRLIGADMQASTAIINEINIHKQLSGHENIVAFVGSSYTAPSTQLGAQYLLLTELCKGGSLVDCFRTNNAPFNPTCVLRIFYQMARAVASLHSQSPPIAHRDIKIENFLIGNDKQIKLCDFGSASTEVLSPTFEWSANQRSMLEDQLNTVTTPMYRSPEMLDTWSNNPIGPKVDIWALGCILYFLCYRKHPYEDGGKLRIINANYMLPPDPQYQCFSDIIRGCLKVNPFERLDITMVLEGLAALAETHNWSLKGPLDLHIMPIESLPAESPLKSSAYSEVYTEPLSAAIPSSYNGHGSLLSSLRGGAGTLLKNIKDTSTKVMQTMQQSLARNDLDISHITSRILVMPCPSDGFESTYKTNNIEDVRLSLESRFVPQKLSIYNFGQRTEPRLPPPVRTVEAGSVYGCPQAHAPNLQGLFTVSADMYNFLNADPKSVVIVQTGDSGGCTAATVICALLMYADLLREPEDAVQVFAVKRHTINLRPSEFRYLYYFGDILRPTPLLPHYKNTTLVSLSCQPVPRMTKARDGCRIYMEVYCNGNLLLSTLQDYEKMRLYQAGPGKIVLPINLTACGDVTVVLFHARKGMVRPQGLKICQFQFNTGFIPEPETLITFTNQDLDDLPDPEQVTPRFCVSLSLAVTDSESPPSHKPPWMPAKPKRSPAALFSSDLEYAEMLDNFVTKPSTRSSPPPGARKSDRVSSPLVLPDVTETAHELPSPMPEPSPPIDLLNLNQQPSDVPAADPLTSAKPSTDASFDLLGAFGDDDSTGIGSAPIPDILPPPPLQQPQPKINNDPFDIFGSVDQGGVPSMKPSGFPPFVGSLPTFVAQPAATTNPSPTHPARASADPFANIADLATGLNLNFNRSTLSGKSPVNTSPQPTQFSSPTHKPSPSSQPQATFMHTPPTPQTLPSTSSIRTPTQPQAVPAQSRPDYSRLHFDSQKAAQQPGTGGSKNSDIFADILGQQGYSFGSKMNQCPRSINEMRKEDLFKDMDPKKVRIMEWTDGKKNNIRALLCSMHTVLWDNAKWQRCEMSTMVTPTEVKKAYRRACLAVHPDKHNGTENEEIAKLIFMELNNAWTDFENDATQQNMFNA

Target ORF information:

RefSeq Version	NM_001275315.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster auxilin (aux), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275315.1

ORF Insert Sequence:

ATGGGCGAGT TCTTTAAGTC GCTCAACCTC AACTACTTCT CCTCTAGCGA GGTCAACGGA 
GCTGCAGGAA ACGGAGCACC AGAGGCCCTC GGTGGCAGGC TGGACAATGA CTTCGTGGGC 
CAGGTCGTTG AAGTCGCTGG ACACAGGCTG CGCATCAAGT GTGTCATCGC CGAAGCCCAA 
GATGTGCAAA CTGGCACAGA ATACGCCCTC AAGCGTCTGA TCGGGGCTGA CATGCAAGCC 
TCCACCGCCA TCATCAACGA GATCAACATC CACAAGCAGC TGTCGGGGCA CGAGAACATT 
GTCGCCTTTG TGGGTTCCAG TTATACCGCC CCATCAACTC AATTGGGAGC CCAGTACTTG 
CTCCTCACCG AGCTGTGTAA AGGCGGATCT CTGGTGGACT GCTTCAGAAC AAACAATGCT 
CCATTCAATC CGACTTGTGT TCTGCGCATC TTCTACCAAA TGGCGCGGGC TGTGGCCAGC 
TTGCACTCAC AGTCACCGCC GATAGCCCAC CGAGATATAA AGATTGAGAA CTTTCTCATT 
GGCAACGACA AACAGATCAA GCTGTGCGAC TTTGGGTCCG CCAGCACGGA GGTCCTGTCG 
CCCACGTTTG AGTGGAGCGC CAACCAGCGC AGCATGCTGG AGGACCAGCT AAACACTGTT 
ACTACTCCGA TGTACCGATC CCCTGAGATG CTCGACACTT GGTCCAACAA CCCGATTGGG 
CCCAAAGTGG ACATATGGGC TCTCGGATGC ATTCTCTACT TCCTGTGCTA CCGCAAGCAT 
CCCTACGAGG ATGGCGGAAA GCTGCGGATC ATTAACGCCA ATTACATGCT TCCGCCAGAT 
CCTCAGTACC AGTGCTTCAG CGATATAATC CGTGGCTGTT TAAAAGTGAA CCCGTTCGAG 
CGCCTGGATA TTACTATGGT GCTGGAGGGC CTTGCAGCCC TCGCTGAGAC ACACAATTGG 
TCGCTCAAAG GACCTCTAGA CCTTCATATA ATGCCCATCG AGTCCCTTCC GGCCGAAAGT 
CCTCTCAAAA GTTCTGCTTA CTCTGAGGTT TACACCGAAC CGCTTTCTGC CGCCATACCA 
TCATCTTATA ATGGACACGG CAGTCTTCTG TCGTCACTGC GTGGTGGCGC TGGAACGCTT 
TTAAAGAACA TTAAAGATAC GTCCACCAAA GTGATGCAAA CAATGCAGCA ATCTCTTGCT 
CGCAACGATC TTGATATAAG CCACATTACC TCTCGCATCC TGGTAATGCC CTGCCCCTCT 
GACGGATTCG AGTCGACGTA CAAGACGAAC AACATCGAAG ACGTCCGACT ATCTCTGGAG 
AGCCGATTTG TCCCACAAAA ATTAAGCATT TACAACTTTG GCCAACGAAC AGAGCCACGG 
CTACCGCCAC CCGTGCGAAC AGTTGAGGCA GGTTCGGTTT ATGGGTGCCC ACAGGCACAT 
GCTCCTAATC TGCAGGGCCT TTTTACCGTA TCAGCCGACA TGTACAATTT TCTAAATGCG 
GACCCCAAAT CTGTCGTTAT CGTGCAGACT GGAGACTCGG GTGGATGCAC TGCTGCCACC 
GTTATATGCG CGCTTCTTAT GTATGCAGAT TTGCTGCGGG AGCCGGAGGA CGCGGTGCAA 
GTCTTCGCCG TTAAGCGGCA TACCATTAAC CTCCGCCCCT CCGAGTTCCG TTACCTGTAT 
TACTTCGGTG ACATCCTGCG GCCCACGCCC CTGCTGCCGC ACTATAAGAA CACAACCCTT 
GTTTCACTCA GCTGCCAGCC TGTGCCCAGA ATGACCAAGG CACGCGACGG CTGTAGGATC 
TATATGGAGG TTTATTGTAA TGGAAACTTG CTACTCAGCA CTCTACAGGA CTATGAAAAA 
ATGCGGTTGT ATCAGGCCGG CCCCGGCAAA ATAGTTCTGC CGATTAACTT GACCGCATGC 
GGTGATGTGA CAGTGGTATT ATTTCACGCC CGCAAAGGAA TGGTGCGACC ACAAGGCCTC 
AAGATATGTC AGTTCCAATT CAACACGGGA TTCATCCCCG AACCAGAAAC GTTGATTACA 
TTCACAAACC AGGACTTGGA TGATCTGCCA GACCCTGAGC AGGTGACACC TCGGTTCTGC 
GTTTCACTTT CTCTGGCTGT TACTGACTCG GAATCACCTC CCAGCCATAA GCCACCATGG 
ATGCCAGCCA AGCCCAAGCG GTCACCCGCC GCTTTGTTCA GCTCAGATTT GGAGTATGCA 
GAAATGCTCG ACAATTTTGT AACCAAGCCT AGCACGCGTT CCTCACCACC ACCAGGTGCC 
CGCAAGAGTG ATCGCGTCAG CTCTCCGCTC GTTTTGCCCG ACGTCACGGA GACCGCCCAC 
GAACTGCCTT CGCCCATGCC AGAGCCATCT CCGCCCATAG ACTTGCTCAA TTTAAACCAG 
CAGCCCAGTG ACGTACCAGC AGCAGATCCG TTAACCAGCG CTAAGCCCAG CACAGATGCC 
AGCTTTGACT TGCTAGGGGC CTTCGGTGAT GATGACTCTA CGGGCATAGG ATCTGCGCCA 
ATTCCTGACA TACTGCCACC ACCGCCACTG CAGCAGCCGC AACCAAAAAT AAACAATGAC 
CCATTCGACA TATTTGGATC TGTGGACCAA GGTGGCGTGC CCAGCATGAA GCCATCAGGC 
TTTCCACCTT TCGTTGGCTC CTTACCAACT TTTGTTGCTC AGCCAGCGGC TACCACCAAT 
CCTTCACCAA CTCATCCGGC AAGGGCATCT GCAGATCCAT TTGCGAACAT CGCCGACCTG 
GCGACAGGGC TCAACTTAAA CTTTAACCGC AGCACGCTAA GTGGAAAGTC TCCCGTCAAC 
ACTAGTCCGC AGCCTACTCA GTTCTCAAGC CCCACTCACA AGCCATCGCC ATCCTCGCAA 
CCACAAGCAA CGTTTATGCA TACACCGCCG ACACCGCAGA CTCTGCCCTC GACATCGTCT 
ATCAGGACGC CGACGCAGCC ACAGGCGGTA CCTGCTCAAT CAAGACCAGA CTACAGTCGA 
CTTCACTTTG ATTCTCAGAA AGCAGCCCAG CAACCGGGCA CTGGCGGGTC CAAGAACTCT 
GACATATTTG CTGACATTCT GGGCCAACAA GGATACTCCT TTGGCAGCAA AATGAACCAG 
TGTCCACGAT CCATCAATGA AATGCGCAAG GAAGATTTGT TCAAGGATAT GGACCCGAAG 
AAAGTGCGGA TTATGGAATG GACTGATGGT AAGAAAAACA ACATCCGTGC GCTTCTCTGC 
TCTATGCACA CAGTGTTGTG GGACAATGCC AAGTGGCAGC GCTGCGAGAT GTCCACCATG 
GTGACACCGA CCGAGGTTAA GAAAGCATAC CGTCGCGCCT GCCTAGCAGT GCATCCTGAT 
AAGCATAATG GAACAGAGAA TGAAGAGATT GCCAAGCTTA TATTTATGGA ACTAAACAAC 
GCATGGACAG ATTTTGAAAA CGATGCCACG CAGCAAAATA TGTTTAATGC GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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aux cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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