Gak gene cDNA ORF clone, Mus musculus(house mouse)

mRNA	Protein	Name
XM_006534907.2	XP_006534970.1	cyclin-G-associated kinase isoform X1
XM_006534905.3	XP_006534968.1	cyclin-G-associated kinase isoform X1
NM_001359923.1	NP_001346852.1	cyclin-G-associated kinase isoform 5
XM_006534905.2	XP_006534968.1	cyclin-G-associated kinase isoform X1
XM_017320853.1	XP_017176342.1	cyclin-G-associated kinase isoform X3
NM_001282052.1	NP_001268981.1	cyclin-G-associated kinase isoform 3
NM_001359922.1	NP_001346851.1	cyclin-G-associated kinase isoform 4
XM_006534907.3	XP_006534970.1	cyclin-G-associated kinase isoform X2
NM_001359924.1	NP_001346853.1	cyclin-G-associated kinase isoform 2
NM_001282051.1	NP_001268980.1	cyclin-G-associated kinase isoform 2
NM_153569.2	NP_705797.1	cyclin-G-associated kinase isoform 1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu83996	NM_001359923.1 Latest version!	Mus musculus cyclin G associated kinase (Gak), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu21899	NM_001359924.1 Latest version!	Mus musculus cyclin G associated kinase (Gak), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$559.30 $799.00
OMu21899	NM_001282051.1 Latest version!	Mus musculus cyclin G associated kinase (Gak), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$559.30 $799.00
OMu21899	XM_006534907.3 Latest version!	Mus musculus cyclin G associated kinase (Gak), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$559.30 $799.00
OMu21889	NM_001282052.1 Latest version!	Mus musculus cyclin G associated kinase (Gak), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$559.30 $799.00
OMu21914	NM_153569.2 Latest version!	Mus musculus cyclin G associated kinase (Gak), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$769.30 $1099.00
OMu81362	NM_001359922.1 Latest version!	Mus musculus cyclin G associated kinase (Gak), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu35450	XM_006534905.3 Latest version!	Mus musculus cyclin G associated kinase (Gak), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu81362	XM_017320853.1 Latest version!	Mus musculus cyclin G associated kinase (Gak), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OMu35450	XM_006534905.2	Mus musculus cyclin G associated kinase (Gak), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu21899	XM_006534907.2	Mus musculus cyclin G associated kinase (Gak), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$559.30 $799.00
Hide Old Accession Versions >>

Gak ( NM_001359923.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001346852.1 Mus musculus cyclin G associated kinase (Gak), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OMu83996
Clone ID Related Accession (Same CDS sequence)	NM_001359923.1
Accession Version	NM_001359923.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2706bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-12
Organism	Mus musculus(house mouse)
Product	cyclin-G-associated kinase isoform 5
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC161813.2 and AK051394.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AK051394.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCACAGGC AGAAACCACC CATCATCCAC AGAGATCTCA AGGTTGAAAA CTTACTGCTT 
AGTAACCAGG GGACCATTAA GCTGTGTGAC TTTGGCAGTG CCACAACCAT CTCCCATTAT 
CCTGACTACA GCTGGAGTGC CCAGAAGCGA GCCATGGTGG AGGAAGAGAT CACAAGGAAC 
ACCACACCCA TGTACAGAAC ACCAGAAATT GTAGACCTGT ATTCCAACTT CCCCATTGGC 
GAAAAGCAGG ATATCTGGGC ACTGGGCTGT ATCTTATACC TGCTGTGTTT CCGGCAGCAT 
CCTTTTGAGG ATGGAGCAAA ACTTCGGATA GTCAATGGGA AGTATTCCAT TCCTGTGAAT 
GACACTCGTT ACACAGTCTT CCATGACCTT ATTCGTGCCA TGCTAAAGGT CAACCCAGAG 
GAGAGGCTGT CCATTGCTGA AGTGGTCCGA CAACTGCAGG AAATTGCAGC AGCTAGGAAT 
GTGAACCCCA AAGCTCCCAT CACAGAGCTG CTGGAGCAGA ATGGTGGCTA TGGGAACTCA 
GGGCCTTCCC GAGCACAACC ACCTTGCGGG GGCACCGTGA ACAGCAGTGG AGTTTTGGCT 
CTGGCAGAGT ATGACCAGCC CTACGGTGGG TTTCTTGATA TTCTGCGGGG TGGGACCGAA 
CGGCTCTTCA CCAACCTCAA GGATACTTCC TCCAAGGTCA TCCAGTCTGT GGCTAACTAT 
GCAAAGGGTG ATCTGGACAT ATCTTACATC ACATCCAGGA TTGCAGTGAT GTCATTCCCA 
GCAGAAGGTG TGGAGTCTGC AATCAAAAAC AATATAGAGG ATGTGCGAAT GTTTCTGGAT 
GCTAAGCATC CAGGACATTA TGCTGTCTAC AACCTTTCTC CAAGGATATA CCGGGCTTCC 
AAGTTTCACA ACCGGGTCAC TGAGTGTGGC TGGGCAGTCA GACGGGCACC ACATCTCCAC 
AGTTTGTATA CTCTATGTAG GAGCATGCAT GCCTGGCTCC GGGAAGACCA CAGGAACGTC 
TGTGTTGTGC ATTGCATGGA TGGGAGAGCT GCATCTGCTG TGGCAGTCTG TGCATTCCTG 
TGCTTCTGCC GTCTCTTCAG CACTGCAGAG GCTGCTGTGT ACATGTTCAG CATGAAGCGC 
TGCCCACCAG GCATTTGGCC ATCCCACAAA AGGTACATTG AATATGTATG TGACATGGTG 
GCAGAGGAGC CCATCACGCC CCATAGCAAG CCAATGCTGG TGAAATCTGT TGTCATGACC 
CCCGTGCCAC TGTTCAGCAA GCAGAGGAAT GGCTGCCGAC CGTTCTGTGA GGTCTACGTT 
GGAGAGGAGC GTGTCACCAC CACGTCGCAG GAATATGACA GAATGAAGGA ATTTAAAATT 
GAGGATGGCA AGGCTGTCAT CCCCCTGGGC GTAACAGTTC AAGGAGACGT GCTCATCATC 
ATCTACCATG CCAGGGCCAC ACTGGGAGGG AGGCTGCAGG CTAAGATGGC GTCCATGAAA 
ATGTTCCAGA TCCAGTTCCA CACTGGGTTC GTGCCTCGAA ACGCAACCAC TGTGAAATTT 
GCAAAATATG ACCTGGATGC TTGTGATATT CAAGAGAAGT ACCCAGATCT GTTCCAGGTG 
AACCTGGAAG TGGAGGTAGA GCCTAGAGAC AGGCCCAGCC GAGAAGCTCC ACCTTGGGAG 
AACACCAGCC TAAGGGGGTT AAACCCCAAG ATCCTCTTTT CCAACAGGGA AGAGCAGCAG 
GACATTCTGT CTAAGTTTGG GAAGCCGGAG CTACCCCGGC AGCCGGGCTC CACAGCTCAG 
TATGATGCTG AGGCAGGGTC TCCGGAGGCT GAGATCACAG AGTCGGACTC ACCGCAGAGC 
AGCAGTACGG ACACCAACCA CTTTCTTCAC ACACTGGATT GGCAGGAGGA AAAAGAACCA 
GAGACTGGGT TAGACAATAC CTCTCCTAAG GAGAGTCAGT CTGTCCTGAT TGCAGACGGA 
GATGGAAGTG AAGTATCAGA TGAAGAAGAG GCTTCATTCC CCAGTGAGGA GAGGAAACCA 
GGAGCTGGAG AAGATACACC AAGGCTGGCT GCTGGGACCA AACAGCAAGA CTTAATATTT 
GATGTGGGCA TGCTGGCTGC CCCACAGGAG CCTGTACAGC CCGAAGAAGG TGTTGATCTC 
CTGGGGCTAC ACTCTGAGGG GGACTTAAGG CCTGCTGCCC CCTTACAGGC TTGTGGGGTC 
CCATCTAGCA ACACTGACCT GTTGAGCTGC CTTCTTGAAC CATCTGATGC TGCTCAAGTA 
GGGCCTCCTG GTGACCTGCT TGGTGGTGAG GCTCCACTGC TGCTAGCAAG CCCAGTTTCT 
CCTCTTGGGC TGCAGAACAA CCTACAAGGA AAAGTCCCTG ACACTGTGGA CCCATTTGAC 
CAGTTCCTGC TGTCATCCAA CTCAGACACC CAGCCCTGCT CCAAGCCTGA TCTCTTTGGA 
GAGTTTCTCA ACTCTGACTC TGTAGCTTCC TCAACTGCCT TCCCATCCAC CCACAGTGCC 
CCACCCCCAT CCTGTAGCAC TGCCTTCCTG CACCTAGGGG ATCTGCCAGC AGAGCCCAGC 
AAGGTAATAG CTTCATCCAG CCACCCAGAT CTGCTAGGAG GATGGGATAC GTGGGCTGAC 
ACAGCTACAC CTGGGCCAGC CTCCATTCCA GTACCAGAAG GTATGACCAC CAAACCCCAC 
TGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001346852.1
CDS	611..3316
Translation	MHRQKPPIIHRDLKVENLLLSNQGTIKLCDFGSATTISHYPDYSWSAQKRAMVEEEITRNTTPMYRTPEIVDLYSNFPIGEKQDIWALGCILYLLCFRQHPFEDGAKLRIVNGKYSIPVNDTRYTVFHDLIRAMLKVNPEERLSIAEVVRQLQEIAAARNVNPKAPITELLEQNGGYGNSGPSRAQPPCGGTVNSSGVLALAEYDQPYGGFLDILRGGTERLFTNLKDTSSKVIQSVANYAKGDLDISYITSRIAVMSFPAEGVESAIKNNIEDVRMFLDAKHPGHYAVYNLSPRIYRASKFHNRVTECGWAVRRAPHLHSLYTLCRSMHAWLREDHRNVCVVHCMDGRAASAVAVCAFLCFCRLFSTAEAAVYMFSMKRCPPGIWPSHKRYIEYVCDMVAEEPITPHSKPMLVKSVVMTPVPLFSKQRNGCRPFCEVYVGEERVTTTSQEYDRMKEFKIEDGKAVIPLGVTVQGDVLIIIYHARATLGGRLQAKMASMKMFQIQFHTGFVPRNATTVKFAKYDLDACDIQEKYPDLFQVNLEVEVEPRDRPSREAPPWENTSLRGLNPKILFSNREEQQDILSKFGKPELPRQPGSTAQYDAEAGSPEAEITESDSPQSSSTDTNHFLHTLDWQEEKEPETGLDNTSPKESQSVLIADGDGSEVSDEEEASFPSEERKPGAGEDTPRLAAGTKQQDLIFDVGMLAAPQEPVQPEEGVDLLGLHSEGDLRPAAPLQACGVPSSNTDLLSCLLEPSDAAQVGPPGDLLGGEAPLLLASPVSPLGLQNNLQGKVPDTVDPFDQFLLSSNSDTQPCSKPDLFGEFLNSDSVASSTAFPSTHSAPPPSCSTAFLHLGDLPAEPSKVIASSSHPDLLGGWDTWADTATPGPASIPVPEGMTTKPHC

Target ORF information:

RefSeq Version	NM_001359923.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus cyclin G associated kinase (Gak), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001359923.1

ORF Insert Sequence:

ATGCACAGGC AGAAACCACC CATCATCCAC AGAGATCTCA AGGTTGAAAA CTTACTGCTT 
AGTAACCAGG GGACCATTAA GCTGTGTGAC TTTGGCAGTG CCACAACCAT CTCCCATTAT 
CCTGACTACA GCTGGAGTGC CCAGAAGCGA GCCATGGTGG AGGAAGAGAT CACAAGGAAC 
ACCACACCCA TGTACAGAAC ACCAGAAATT GTAGACCTGT ATTCCAACTT CCCCATTGGC 
GAAAAGCAGG ATATCTGGGC ACTGGGCTGT ATCTTATACC TGCTGTGTTT CCGGCAGCAT 
CCTTTTGAGG ATGGAGCAAA ACTTCGGATA GTCAATGGGA AGTATTCCAT TCCTGTGAAT 
GACACTCGTT ACACAGTCTT CCATGACCTT ATTCGTGCCA TGCTAAAGGT CAACCCAGAG 
GAGAGGCTGT CCATTGCTGA AGTGGTCCGA CAACTGCAGG AAATTGCAGC AGCTAGGAAT 
GTGAACCCCA AAGCTCCCAT CACAGAGCTG CTGGAGCAGA ATGGTGGCTA TGGGAACTCA 
GGGCCTTCCC GAGCACAACC ACCTTGCGGG GGCACCGTGA ACAGCAGTGG AGTTTTGGCT 
CTGGCAGAGT ATGACCAGCC CTACGGTGGG TTTCTTGATA TTCTGCGGGG TGGGACCGAA 
CGGCTCTTCA CCAACCTCAA GGATACTTCC TCCAAGGTCA TCCAGTCTGT GGCTAACTAT 
GCAAAGGGTG ATCTGGACAT ATCTTACATC ACATCCAGGA TTGCAGTGAT GTCATTCCCA 
GCAGAAGGTG TGGAGTCTGC AATCAAAAAC AATATAGAGG ATGTGCGAAT GTTTCTGGAT 
GCTAAGCATC CAGGACATTA TGCTGTCTAC AACCTTTCTC CAAGGATATA CCGGGCTTCC 
AAGTTTCACA ACCGGGTCAC TGAGTGTGGC TGGGCAGTCA GACGGGCACC ACATCTCCAC 
AGTTTGTATA CTCTATGTAG GAGCATGCAT GCCTGGCTCC GGGAAGACCA CAGGAACGTC 
TGTGTTGTGC ATTGCATGGA TGGGAGAGCT GCATCTGCTG TGGCAGTCTG TGCATTCCTG 
TGCTTCTGCC GTCTCTTCAG CACTGCAGAG GCTGCTGTGT ACATGTTCAG CATGAAGCGC 
TGCCCACCAG GCATTTGGCC ATCCCACAAA AGGTACATTG AATATGTATG TGACATGGTG 
GCAGAGGAGC CCATCACGCC CCATAGCAAG CCAATGCTGG TGAAATCTGT TGTCATGACC 
CCCGTGCCAC TGTTCAGCAA GCAGAGGAAT GGCTGCCGAC CGTTCTGTGA GGTCTACGTT 
GGAGAGGAGC GTGTCACCAC CACGTCGCAG GAATATGACA GAATGAAGGA ATTTAAAATT 
GAGGATGGCA AGGCTGTCAT CCCCCTGGGC GTAACAGTTC AAGGAGACGT GCTCATCATC 
ATCTACCATG CCAGGGCCAC ACTGGGAGGG AGGCTGCAGG CTAAGATGGC GTCCATGAAA 
ATGTTCCAGA TCCAGTTCCA CACTGGGTTC GTGCCTCGAA ACGCAACCAC TGTGAAATTT 
GCAAAATATG ACCTGGATGC TTGTGATATT CAAGAGAAGT ACCCAGATCT GTTCCAGGTG 
AACCTGGAAG TGGAGGTAGA GCCTAGAGAC AGGCCCAGCC GAGAAGCTCC ACCTTGGGAG 
AACACCAGCC TAAGGGGGTT AAACCCCAAG ATCCTCTTTT CCAACAGGGA AGAGCAGCAG 
GACATTCTGT CTAAGTTTGG GAAGCCGGAG CTACCCCGGC AGCCGGGCTC CACAGCTCAG 
TATGATGCTG AGGCAGGGTC TCCGGAGGCT GAGATCACAG AGTCGGACTC ACCGCAGAGC 
AGCAGTACGG ACACCAACCA CTTTCTTCAC ACACTGGATT GGCAGGAGGA AAAAGAACCA 
GAGACTGGGT TAGACAATAC CTCTCCTAAG GAGAGTCAGT CTGTCCTGAT TGCAGACGGA 
GATGGAAGTG AAGTATCAGA TGAAGAAGAG GCTTCATTCC CCAGTGAGGA GAGGAAACCA 
GGAGCTGGAG AAGATACACC AAGGCTGGCT GCTGGGACCA AACAGCAAGA CTTAATATTT 
GATGTGGGCA TGCTGGCTGC CCCACAGGAG CCTGTACAGC CCGAAGAAGG TGTTGATCTC 
CTGGGGCTAC ACTCTGAGGG GGACTTAAGG CCTGCTGCCC CCTTACAGGC TTGTGGGGTC 
CCATCTAGCA ACACTGACCT GTTGAGCTGC CTTCTTGAAC CATCTGATGC TGCTCAAGTA 
GGGCCTCCTG GTGACCTGCT TGGTGGTGAG GCTCCACTGC TGCTAGCAAG CCCAGTTTCT 
CCTCTTGGGC TGCAGAACAA CCTACAAGGA AAAGTCCCTG ACACTGTGGA CCCATTTGAC 
CAGTTCCTGC TGTCATCCAA CTCAGACACC CAGCCCTGCT CCAAGCCTGA TCTCTTTGGA 
GAGTTTCTCA ACTCTGACTC TGTAGCTTCC TCAACTGCCT TCCCATCCAC CCACAGTGCC 
CCACCCCCAT CCTGTAGCAC TGCCTTCCTG CACCTAGGGG ATCTGCCAGC AGAGCCCAGC 
AAGGTAATAG CTTCATCCAG CCACCCAGAT CTGCTAGGAG GATGGGATAC GTGGGCTGAC 
ACAGCTACAC CTGGGCCAGC CTCCATTCCA GTACCAGAAG GTATGACCAC CAAACCCCAC 
TGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gak ( NM_001359924.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001346853.1 Mus musculus cyclin G associated kinase (Gak), transcript variant 6, mRNA.

Price & Availability↑

CloneID	OMu21899
Clone ID Related Accession (Same CDS sequence)	XM_006534907.2 , XM_006534907.3 , NM_001359924.1 , NM_001282051.1
Accession Version	NM_001359924.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3435bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-12
Organism	Mus musculus(house mouse)
Product	cyclin-G-associated kinase isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC161813.2, AK150112.1 and AC133896.7. On Jan 19, 2018 this sequence version replaced XM_006534907.3. Transcript Variant: This variant (6), as well as variant 2, encodes isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AK159767.1, BC065109.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCACAGGC AGAAACCACC CATCATCCAC AGAGATCTCA AGGTTGAAAA CTTACTGCTT 
AGTAACCAGG GGACCATTAA GCTGTGTGAC TTTGGCAGTG CCACAACCAT CTCCCATTAT 
CCTGACTACA GCTGGAGTGC CCAGAAGCGA GCCATGGTGG AGGAAGAGAT CACAAGGAAC 
ACCACACCCA TGTACAGAAC ACCAGAAATT GTAGACCTGT ATTCCAACTT CCCCATTGGC 
GAAAAGCAGG ATATCTGGGC ACTGGGCTGT ATCTTATACC TGCTGTGTTT CCGGCAGCAT 
CCTTTTGAGG ATGGAGCAAA ACTTCGGATA GTCAATGGGA AGTATTCCAT TCCTGTGAAT 
GACACTCGTT ACACAGTCTT CCATGACCTT ATTCGTGCCA TGCTAAAGGT CAACCCAGAG 
GAGAGGCTGT CCATTGCTGA AGTGGTCCGA CAACTGCAGG AAATTGCAGC AGCTAGGAAT 
GTGAACCCCA AAGCTCCCAT CACAGAGCTG CTGGAGCAGA ATGGTGGCTA TGGGAACTCA 
GGGCCTTCCC GAGCACAACC ACCTTGCGGG GGCACCGTGA ACAGCAGTGG AGTTTTGGCT 
CTGGCAGAGT ATGACCAGCC CTACGGTGGG TTTCTTGATA TTCTGCGGGG TGGGACCGAA 
CGGCTCTTCA CCAACCTCAA GGATACTTCC TCCAAGGTCA TCCAGTCTGT GGCTAACTAT 
GCAAAGGGTG ATCTGGACAT ATCTTACATC ACATCCAGGA TTGCAGTGAT GTCATTCCCA 
GCAGAAGGTG TGGAGTCTGC AATCAAAAAC AATATAGAGG ATGTGCGAAT GTTTCTGGAT 
GCTAAGCATC CAGGACATTA TGCTGTCTAC AACCTTTCTC CAAGGATATA CCGGGCTTCC 
AAGTTTCACA ACCGGGTCAC TGAGTGTGGC TGGGCAGTCA GACGGGCACC ACATCTCCAC 
AGTTTGTATA CTCTATGTAG GAGCATGCAT GCCTGGCTCC GGGAAGACCA CAGGAACGTC 
TGTGTTGTGC ATTGCATGGA TGGGAGAGCT GCATCTGCTG TGGCAGTCTG TGCATTCCTG 
TGCTTCTGCC GTCTCTTCAG CACTGCAGAG GCTGCTGTGT ACATGTTCAG CATGAAGCGC 
TGCCCACCAG GCATTTGGCC ATCCCACAAA AGGTACATTG AATATGTATG TGACATGGTG 
GCAGAGGAGC CCATCACGCC CCATAGCAAG CCAATGCTGG TGAAATCTGT TGTCATGACC 
CCCGTGCCAC TGTTCAGCAA GCAGAGGAAT GGCTGCCGAC CGTTCTGTGA GGTCTACGTT 
GGAGAGGAGC GTGTCACCAC CACGTCGCAG GAATATGACA GAATGAAGGA ATTTAAAATT 
GAGGATGGCA AGGCTGTCAT CCCCCTGGGC GTAACAGTTC AAGGAGACGT GCTCATCATC 
ATCTACCATG CCAGGGCCAC ACTGGGAGGG AGGCTGCAGG CTAAGATGGC GTCCATGAAA 
ATGTTCCAGA TCCAGTTCCA CACTGGGTTC GTGCCTCGAA ACGCAACCAC TGTGAAATTT 
GCAAAATATG ACCTGGATGC TTGTGATATT CAAGAGAAGT ACCCAGATCT GTTCCAGGTG 
AACCTGGAAG TGGAGGTAGA GCCTAGAGAC AGGCCCAGCC GAGAAGCTCC ACCTTGGGAG 
AACACCAGCC TAAGGGGGTT AAACCCCAAG ATCCTCTTTT CCAACAGGGA AGAGCAGCAG 
GACATTCTGT CTAAGTTTGG GAAGCCGGAG CTACCCCGGC AGCCGGGCTC CACAGCTCAG 
TATGATGCTG AGGCAGGGTC TCCGGAGGCT GAGATCACAG AGTCGGACTC ACCGCAGAGC 
AGCAGTACGG ACACCAACCA CTTTCTTCAC ACACTGGATT GGCAGGAGGA AAAAGAACCA 
GAGACTGGGT TAGACAATAC CTCTCCTAAG GAGAGTCAGT CTGTCCTGAT TGCAGACGGA 
GATGGAAGTG AAGTATCAGA TGAAGAAGAG GCTTCATTCC CCAGTGAGGA GAGGAAACCA 
GGAGCTGGAG AAGATACACC AAGGCTGGCT GCTGGGACCA AACAGCAAGA CTTAATATTT 
GATGTGGGCA TGCTGGCTGC CCCACAGGAG CCTGTACAGC CCGAAGAAGG TGTTGATCTC 
CTGGGGCTAC ACTCTGAGGG GGACTTAAGG CCTGCTGCCC CCTTACAGGC TTGTGGGGTC 
CCATCTAGCA ACACTGACCT GTTGAGCTGC CTTCTTGAAC CATCTGATGC TGCTCAAGTA 
GGGCCTCCTG GTGACCTGCT TGGTGGTGAG GCTCCACTGC TGCTAGCAAG CCCAGTTTCT 
CCTCTTGGGC TGCAGAACAA CCTACAAGGA AAAGTCCCTG ACACTGTGGA CCCATTTGAC 
CAGTTCCTGC TGTCATCCAA CTCAGACACC CAGCCCTGCT CCAAGCCTGA TCTCTTTGGA 
GAGTTTCTCA ACTCTGACTC TGTAGCTTCC TCAACTGCCT TCCCATCCAC CCACAGTGCC 
CCACCCCCAT CCTGTAGCAC TGCCTTCCTG CACCTAGGGG ATCTGCCAGC AGAGCCCAGC 
AAGGTAATAG CTTCATCCAG CCACCCAGAT CTGCTAGGAG GATGGGATAC GTGGGCTGAC 
ACAGCTACAC CTGGGCCAGC CTCCATTCCA GTACCAGAAG GTACCCTCTT CTCTTCTGCA 
GGTCACCCAG CCCCTCCTGG CCCCAACCCC AGCCAAACCA AGTCTCAGAA CCTTGACCCA 
TTTGCTGATC TCAGTGATCT CAGCTCTAGC CTCCAAGGCC TGCCTGCTGG ACTTCCTGCA 
GGGGGCTTTG TTGGCGCACC AGCCCCCACT CAAAAGAGCA ACAGCCCCTG GCAGGCAAAT 
CGTCCCACAG CCCCTGGAAC CTCATGGACC CCCCAGGCCA AGCCAGCCCC CAGAGCCTCT 
GAACAGCTAA GGTCTCACTT CAGTGTGATC GGGGCCCGAG AAGAGAGGGG CGTCCGTGTG 
CCCAGCTTTG CCCAAAAGCC AAAGGTCTCA GAAAATGATT TTGAAGATCT TCTGCCTAAT 
CAAGGCTTCT CTAAGTCTGA CAAGAAGGGG CCAAAGACCA TGGCAGAGAT GCGGAAACAG 
GAACTTGCCA GAGATACAGA CCCACTCAAA TTGAAGCTTT TGGACTGGAT TGAAGGCAAG 
GAGAGGAACA TTCGTGCACT GCTGTCCACT CTGCACACGG TATTGTGGGA TGGGGAGAGC 
CGCTGGACAC CTGTGAGTAT GGCTGACCTG GTGACTCCAG AGCAGGTGAA GAAGCAGTAC 
CGCCGTGCAG TGCTGGTAGT GCATCCTGAT AAGGCCACAG GGCAGCCGTA TGAACAGTAT 
GCCAAGATGA TCTTCATGGA GCTGAATGAT GCATGGTCTG AGTTTGAAAA CCAGGGCTCG 
AGGCCCCTCT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001346853.1
CDS	441..3875
Translation	MHRQKPPIIHRDLKVENLLLSNQGTIKLCDFGSATTISHYPDYSWSAQKRAMVEEEITRNTTPMYRTPEIVDLYSNFPIGEKQDIWALGCILYLLCFRQHPFEDGAKLRIVNGKYSIPVNDTRYTVFHDLIRAMLKVNPEERLSIAEVVRQLQEIAAARNVNPKAPITELLEQNGGYGNSGPSRAQPPCGGTVNSSGVLALAEYDQPYGGFLDILRGGTERLFTNLKDTSSKVIQSVANYAKGDLDISYITSRIAVMSFPAEGVESAIKNNIEDVRMFLDAKHPGHYAVYNLSPRIYRASKFHNRVTECGWAVRRAPHLHSLYTLCRSMHAWLREDHRNVCVVHCMDGRAASAVAVCAFLCFCRLFSTAEAAVYMFSMKRCPPGIWPSHKRYIEYVCDMVAEEPITPHSKPMLVKSVVMTPVPLFSKQRNGCRPFCEVYVGEERVTTTSQEYDRMKEFKIEDGKAVIPLGVTVQGDVLIIIYHARATLGGRLQAKMASMKMFQIQFHTGFVPRNATTVKFAKYDLDACDIQEKYPDLFQVNLEVEVEPRDRPSREAPPWENTSLRGLNPKILFSNREEQQDILSKFGKPELPRQPGSTAQYDAEAGSPEAEITESDSPQSSSTDTNHFLHTLDWQEEKEPETGLDNTSPKESQSVLIADGDGSEVSDEEEASFPSEERKPGAGEDTPRLAAGTKQQDLIFDVGMLAAPQEPVQPEEGVDLLGLHSEGDLRPAAPLQACGVPSSNTDLLSCLLEPSDAAQVGPPGDLLGGEAPLLLASPVSPLGLQNNLQGKVPDTVDPFDQFLLSSNSDTQPCSKPDLFGEFLNSDSVASSTAFPSTHSAPPPSCSTAFLHLGDLPAEPSKVIASSSHPDLLGGWDTWADTATPGPASIPVPEGTLFSSAGHPAPPGPNPSQTKSQNLDPFADLSDLSSSLQGLPAGLPAGGFVGAPAPTQKSNSPWQANRPTAPGTSWTPQAKPAPRASEQLRSHFSVIGAREERGVRVPSFAQKPKVSENDFEDLLPNQGFSKSDKKGPKTMAEMRKQELARDTDPLKLKLLDWIEGKERNIRALLSTLHTVLWDGESRWTPVSMADLVTPEQVKKQYRRAVLVVHPDKATGQPYEQYAKMIFMELNDAWSEFENQGSRPLF

Target ORF information:

RefSeq Version	NM_001359924.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus cyclin G associated kinase (Gak), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001359924.1

ORF Insert Sequence:

ATGCACAGGC AGAAACCACC CATCATCCAC AGAGATCTCA AGGTTGAAAA CTTACTGCTT 
AGTAACCAGG GGACCATTAA GCTGTGTGAC TTTGGCAGTG CCACAACCAT CTCCCATTAT 
CCTGACTACA GCTGGAGTGC CCAGAAGCGA GCCATGGTGG AGGAAGAGAT CACAAGGAAC 
ACCACACCCA TGTACAGAAC ACCAGAAATT GTAGACCTGT ATTCCAACTT CCCCATTGGC 
GAAAAGCAGG ATATCTGGGC ACTGGGCTGT ATCTTATACC TGCTGTGTTT CCGGCAGCAT 
CCTTTTGAGG ATGGAGCAAA ACTTCGGATA GTCAATGGGA AGTATTCCAT TCCTGTGAAT 
GACACTCGTT ACACAGTCTT CCATGACCTT ATTCGTGCCA TGCTAAAGGT CAACCCAGAG 
GAGAGGCTGT CCATTGCTGA AGTGGTCCGA CAACTGCAGG AAATTGCAGC AGCTAGGAAT 
GTGAACCCCA AAGCTCCCAT CACAGAGCTG CTGGAGCAGA ATGGTGGCTA TGGGAACTCA 
GGGCCTTCCC GAGCACAACC ACCTTGCGGG GGCACCGTGA ACAGCAGTGG AGTTTTGGCT 
CTGGCAGAGT ATGACCAGCC CTACGGTGGG TTTCTTGATA TTCTGCGGGG TGGGACCGAA 
CGGCTCTTCA CCAACCTCAA GGATACTTCC TCCAAGGTCA TCCAGTCTGT GGCTAACTAT 
GCAAAGGGTG ATCTGGACAT ATCTTACATC ACATCCAGGA TTGCAGTGAT GTCATTCCCA 
GCAGAAGGTG TGGAGTCTGC AATCAAAAAC AATATAGAGG ATGTGCGAAT GTTTCTGGAT 
GCTAAGCATC CAGGACATTA TGCTGTCTAC AACCTTTCTC CAAGGATATA CCGGGCTTCC 
AAGTTTCACA ACCGGGTCAC TGAGTGTGGC TGGGCAGTCA GACGGGCACC ACATCTCCAC 
AGTTTGTATA CTCTATGTAG GAGCATGCAT GCCTGGCTCC GGGAAGACCA CAGGAACGTC 
TGTGTTGTGC ATTGCATGGA TGGGAGAGCT GCATCTGCTG TGGCAGTCTG TGCATTCCTG 
TGCTTCTGCC GTCTCTTCAG CACTGCAGAG GCTGCTGTGT ACATGTTCAG CATGAAGCGC 
TGCCCACCAG GCATTTGGCC ATCCCACAAA AGGTACATTG AATATGTATG TGACATGGTG 
GCAGAGGAGC CCATCACGCC CCATAGCAAG CCAATGCTGG TGAAATCTGT TGTCATGACC 
CCCGTGCCAC TGTTCAGCAA GCAGAGGAAT GGCTGCCGAC CGTTCTGTGA GGTCTACGTT 
GGAGAGGAGC GTGTCACCAC CACGTCGCAG GAATATGACA GAATGAAGGA ATTTAAAATT 
GAGGATGGCA AGGCTGTCAT CCCCCTGGGC GTAACAGTTC AAGGAGACGT GCTCATCATC 
ATCTACCATG CCAGGGCCAC ACTGGGAGGG AGGCTGCAGG CTAAGATGGC GTCCATGAAA 
ATGTTCCAGA TCCAGTTCCA CACTGGGTTC GTGCCTCGAA ACGCAACCAC TGTGAAATTT 
GCAAAATATG ACCTGGATGC TTGTGATATT CAAGAGAAGT ACCCAGATCT GTTCCAGGTG 
AACCTGGAAG TGGAGGTAGA GCCTAGAGAC AGGCCCAGCC GAGAAGCTCC ACCTTGGGAG 
AACACCAGCC TAAGGGGGTT AAACCCCAAG ATCCTCTTTT CCAACAGGGA AGAGCAGCAG 
GACATTCTGT CTAAGTTTGG GAAGCCGGAG CTACCCCGGC AGCCGGGCTC CACAGCTCAG 
TATGATGCTG AGGCAGGGTC TCCGGAGGCT GAGATCACAG AGTCGGACTC ACCGCAGAGC 
AGCAGTACGG ACACCAACCA CTTTCTTCAC ACACTGGATT GGCAGGAGGA AAAAGAACCA 
GAGACTGGGT TAGACAATAC CTCTCCTAAG GAGAGTCAGT CTGTCCTGAT TGCAGACGGA 
GATGGAAGTG AAGTATCAGA TGAAGAAGAG GCTTCATTCC CCAGTGAGGA GAGGAAACCA 
GGAGCTGGAG AAGATACACC AAGGCTGGCT GCTGGGACCA AACAGCAAGA CTTAATATTT 
GATGTGGGCA TGCTGGCTGC CCCACAGGAG CCTGTACAGC CCGAAGAAGG TGTTGATCTC 
CTGGGGCTAC ACTCTGAGGG GGACTTAAGG CCTGCTGCCC CCTTACAGGC TTGTGGGGTC 
CCATCTAGCA ACACTGACCT GTTGAGCTGC CTTCTTGAAC CATCTGATGC TGCTCAAGTA 
GGGCCTCCTG GTGACCTGCT TGGTGGTGAG GCTCCACTGC TGCTAGCAAG CCCAGTTTCT 
CCTCTTGGGC TGCAGAACAA CCTACAAGGA AAAGTCCCTG ACACTGTGGA CCCATTTGAC 
CAGTTCCTGC TGTCATCCAA CTCAGACACC CAGCCCTGCT CCAAGCCTGA TCTCTTTGGA 
GAGTTTCTCA ACTCTGACTC TGTAGCTTCC TCAACTGCCT TCCCATCCAC CCACAGTGCC 
CCACCCCCAT CCTGTAGCAC TGCCTTCCTG CACCTAGGGG ATCTGCCAGC AGAGCCCAGC 
AAGGTAATAG CTTCATCCAG CCACCCAGAT CTGCTAGGAG GATGGGATAC GTGGGCTGAC 
ACAGCTACAC CTGGGCCAGC CTCCATTCCA GTACCAGAAG GTACCCTCTT CTCTTCTGCA 
GGTCACCCAG CCCCTCCTGG CCCCAACCCC AGCCAAACCA AGTCTCAGAA CCTTGACCCA 
TTTGCTGATC TCAGTGATCT CAGCTCTAGC CTCCAAGGCC TGCCTGCTGG ACTTCCTGCA 
GGGGGCTTTG TTGGCGCACC AGCCCCCACT CAAAAGAGCA ACAGCCCCTG GCAGGCAAAT 
CGTCCCACAG CCCCTGGAAC CTCATGGACC CCCCAGGCCA AGCCAGCCCC CAGAGCCTCT 
GAACAGCTAA GGTCTCACTT CAGTGTGATC GGGGCCCGAG AAGAGAGGGG CGTCCGTGTG 
CCCAGCTTTG CCCAAAAGCC AAAGGTCTCA GAAAATGATT TTGAAGATCT TCTGCCTAAT 
CAAGGCTTCT CTAAGTCTGA CAAGAAGGGG CCAAAGACCA TGGCAGAGAT GCGGAAACAG 
GAACTTGCCA GAGATACAGA CCCACTCAAA TTGAAGCTTT TGGACTGGAT TGAAGGCAAG 
GAGAGGAACA TTCGTGCACT GCTGTCCACT CTGCACACGG TATTGTGGGA TGGGGAGAGC 
CGCTGGACAC CTGTGAGTAT GGCTGACCTG GTGACTCCAG AGCAGGTGAA GAAGCAGTAC 
CGCCGTGCAG TGCTGGTAGT GCATCCTGAT AAGGCCACAG GGCAGCCGTA TGAACAGTAT 
GCCAAGATGA TCTTCATGGA GCTGAATGAT GCATGGTCTG AGTTTGAAAA CCAGGGCTCG 
AGGCCCCTCT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gak ( NM_001282051.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001268980.1 Mus musculus cyclin G associated kinase (Gak), transcript variant 2, mRNA.

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CloneID	OMu21899
Clone ID Related Accession (Same CDS sequence)	XM_006534907.2 , XM_006534907.3 , NM_001359924.1 , NM_001282051.1
Accession Version	NM_001282051.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3435bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-12
Organism	Mus musculus(house mouse)
Product	cyclin-G-associated kinase isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY312464.1, BC065109.1, AK166682.1, AI466057.1 and AC133896.7. Transcript Variant: This variant (2) differs in its 5' UTR and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus, compared to isoform 1. Variants 2 and 6 both encode the same isoform (2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC065109.1, SRR1660817.69011.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCACAGGC AGAAACCACC CATCATCCAC AGAGATCTCA AGGTTGAAAA CTTACTGCTT 
AGTAACCAGG GGACCATTAA GCTGTGTGAC TTTGGCAGTG CCACAACCAT CTCCCATTAT 
CCTGACTACA GCTGGAGTGC CCAGAAGCGA GCCATGGTGG AGGAAGAGAT CACAAGGAAC 
ACCACACCCA TGTACAGAAC ACCAGAAATT GTAGACCTGT ATTCCAACTT CCCCATTGGC 
GAAAAGCAGG ATATCTGGGC ACTGGGCTGT ATCTTATACC TGCTGTGTTT CCGGCAGCAT 
CCTTTTGAGG ATGGAGCAAA ACTTCGGATA GTCAATGGGA AGTATTCCAT TCCTGTGAAT 
GACACTCGTT ACACAGTCTT CCATGACCTT ATTCGTGCCA TGCTAAAGGT CAACCCAGAG 
GAGAGGCTGT CCATTGCTGA AGTGGTCCGA CAACTGCAGG AAATTGCAGC AGCTAGGAAT 
GTGAACCCCA AAGCTCCCAT CACAGAGCTG CTGGAGCAGA ATGGTGGCTA TGGGAACTCA 
GGGCCTTCCC GAGCACAACC ACCTTGCGGG GGCACCGTGA ACAGCAGTGG AGTTTTGGCT 
CTGGCAGAGT ATGACCAGCC CTACGGTGGG TTTCTTGATA TTCTGCGGGG TGGGACCGAA 
CGGCTCTTCA CCAACCTCAA GGATACTTCC TCCAAGGTCA TCCAGTCTGT GGCTAACTAT 
GCAAAGGGTG ATCTGGACAT ATCTTACATC ACATCCAGGA TTGCAGTGAT GTCATTCCCA 
GCAGAAGGTG TGGAGTCTGC AATCAAAAAC AATATAGAGG ATGTGCGAAT GTTTCTGGAT 
GCTAAGCATC CAGGACATTA TGCTGTCTAC AACCTTTCTC CAAGGATATA CCGGGCTTCC 
AAGTTTCACA ACCGGGTCAC TGAGTGTGGC TGGGCAGTCA GACGGGCACC ACATCTCCAC 
AGTTTGTATA CTCTATGTAG GAGCATGCAT GCCTGGCTCC GGGAAGACCA CAGGAACGTC 
TGTGTTGTGC ATTGCATGGA TGGGAGAGCT GCATCTGCTG TGGCAGTCTG TGCATTCCTG 
TGCTTCTGCC GTCTCTTCAG CACTGCAGAG GCTGCTGTGT ACATGTTCAG CATGAAGCGC 
TGCCCACCAG GCATTTGGCC ATCCCACAAA AGGTACATTG AATATGTATG TGACATGGTG 
GCAGAGGAGC CCATCACGCC CCATAGCAAG CCAATGCTGG TGAAATCTGT TGTCATGACC 
CCCGTGCCAC TGTTCAGCAA GCAGAGGAAT GGCTGCCGAC CGTTCTGTGA GGTCTACGTT 
GGAGAGGAGC GTGTCACCAC CACGTCGCAG GAATATGACA GAATGAAGGA ATTTAAAATT 
GAGGATGGCA AGGCTGTCAT CCCCCTGGGC GTAACAGTTC AAGGAGACGT GCTCATCATC 
ATCTACCATG CCAGGGCCAC ACTGGGAGGG AGGCTGCAGG CTAAGATGGC GTCCATGAAA 
ATGTTCCAGA TCCAGTTCCA CACTGGGTTC GTGCCTCGAA ACGCAACCAC TGTGAAATTT 
GCAAAATATG ACCTGGATGC TTGTGATATT CAAGAGAAGT ACCCAGATCT GTTCCAGGTG 
AACCTGGAAG TGGAGGTAGA GCCTAGAGAC AGGCCCAGCC GAGAAGCTCC ACCTTGGGAG 
AACACCAGCC TAAGGGGGTT AAACCCCAAG ATCCTCTTTT CCAACAGGGA AGAGCAGCAG 
GACATTCTGT CTAAGTTTGG GAAGCCGGAG CTACCCCGGC AGCCGGGCTC CACAGCTCAG 
TATGATGCTG AGGCAGGGTC TCCGGAGGCT GAGATCACAG AGTCGGACTC ACCGCAGAGC 
AGCAGTACGG ACACCAACCA CTTTCTTCAC ACACTGGATT GGCAGGAGGA AAAAGAACCA 
GAGACTGGGT TAGACAATAC CTCTCCTAAG GAGAGTCAGT CTGTCCTGAT TGCAGACGGA 
GATGGAAGTG AAGTATCAGA TGAAGAAGAG GCTTCATTCC CCAGTGAGGA GAGGAAACCA 
GGAGCTGGAG AAGATACACC AAGGCTGGCT GCTGGGACCA AACAGCAAGA CTTAATATTT 
GATGTGGGCA TGCTGGCTGC CCCACAGGAG CCTGTACAGC CCGAAGAAGG TGTTGATCTC 
CTGGGGCTAC ACTCTGAGGG GGACTTAAGG CCTGCTGCCC CCTTACAGGC TTGTGGGGTC 
CCATCTAGCA ACACTGACCT GTTGAGCTGC CTTCTTGAAC CATCTGATGC TGCTCAAGTA 
GGGCCTCCTG GTGACCTGCT TGGTGGTGAG GCTCCACTGC TGCTAGCAAG CCCAGTTTCT 
CCTCTTGGGC TGCAGAACAA CCTACAAGGA AAAGTCCCTG ACACTGTGGA CCCATTTGAC 
CAGTTCCTGC TGTCATCCAA CTCAGACACC CAGCCCTGCT CCAAGCCTGA TCTCTTTGGA 
GAGTTTCTCA ACTCTGACTC TGTAGCTTCC TCAACTGCCT TCCCATCCAC CCACAGTGCC 
CCACCCCCAT CCTGTAGCAC TGCCTTCCTG CACCTAGGGG ATCTGCCAGC AGAGCCCAGC 
AAGGTAATAG CTTCATCCAG CCACCCAGAT CTGCTAGGAG GATGGGATAC GTGGGCTGAC 
ACAGCTACAC CTGGGCCAGC CTCCATTCCA GTACCAGAAG GTACCCTCTT CTCTTCTGCA 
GGTCACCCAG CCCCTCCTGG CCCCAACCCC AGCCAAACCA AGTCTCAGAA CCTTGACCCA 
TTTGCTGATC TCAGTGATCT CAGCTCTAGC CTCCAAGGCC TGCCTGCTGG ACTTCCTGCA 
GGGGGCTTTG TTGGCGCACC AGCCCCCACT CAAAAGAGCA ACAGCCCCTG GCAGGCAAAT 
CGTCCCACAG CCCCTGGAAC CTCATGGACC CCCCAGGCCA AGCCAGCCCC CAGAGCCTCT 
GAACAGCTAA GGTCTCACTT CAGTGTGATC GGGGCCCGAG AAGAGAGGGG CGTCCGTGTG 
CCCAGCTTTG CCCAAAAGCC AAAGGTCTCA GAAAATGATT TTGAAGATCT TCTGCCTAAT 
CAAGGCTTCT CTAAGTCTGA CAAGAAGGGG CCAAAGACCA TGGCAGAGAT GCGGAAACAG 
GAACTTGCCA GAGATACAGA CCCACTCAAA TTGAAGCTTT TGGACTGGAT TGAAGGCAAG 
GAGAGGAACA TTCGTGCACT GCTGTCCACT CTGCACACGG TATTGTGGGA TGGGGAGAGC 
CGCTGGACAC CTGTGAGTAT GGCTGACCTG GTGACTCCAG AGCAGGTGAA GAAGCAGTAC 
CGCCGTGCAG TGCTGGTAGT GCATCCTGAT AAGGCCACAG GGCAGCCGTA TGAACAGTAT 
GCCAAGATGA TCTTCATGGA GCTGAATGAT GCATGGTCTG AGTTTGAAAA CCAGGGCTCG 
AGGCCCCTCT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001268980.1
CDS	611..4045
Translation	MHRQKPPIIHRDLKVENLLLSNQGTIKLCDFGSATTISHYPDYSWSAQKRAMVEEEITRNTTPMYRTPEIVDLYSNFPIGEKQDIWALGCILYLLCFRQHPFEDGAKLRIVNGKYSIPVNDTRYTVFHDLIRAMLKVNPEERLSIAEVVRQLQEIAAARNVNPKAPITELLEQNGGYGNSGPSRAQPPCGGTVNSSGVLALAEYDQPYGGFLDILRGGTERLFTNLKDTSSKVIQSVANYAKGDLDISYITSRIAVMSFPAEGVESAIKNNIEDVRMFLDAKHPGHYAVYNLSPRIYRASKFHNRVTECGWAVRRAPHLHSLYTLCRSMHAWLREDHRNVCVVHCMDGRAASAVAVCAFLCFCRLFSTAEAAVYMFSMKRCPPGIWPSHKRYIEYVCDMVAEEPITPHSKPMLVKSVVMTPVPLFSKQRNGCRPFCEVYVGEERVTTTSQEYDRMKEFKIEDGKAVIPLGVTVQGDVLIIIYHARATLGGRLQAKMASMKMFQIQFHTGFVPRNATTVKFAKYDLDACDIQEKYPDLFQVNLEVEVEPRDRPSREAPPWENTSLRGLNPKILFSNREEQQDILSKFGKPELPRQPGSTAQYDAEAGSPEAEITESDSPQSSSTDTNHFLHTLDWQEEKEPETGLDNTSPKESQSVLIADGDGSEVSDEEEASFPSEERKPGAGEDTPRLAAGTKQQDLIFDVGMLAAPQEPVQPEEGVDLLGLHSEGDLRPAAPLQACGVPSSNTDLLSCLLEPSDAAQVGPPGDLLGGEAPLLLASPVSPLGLQNNLQGKVPDTVDPFDQFLLSSNSDTQPCSKPDLFGEFLNSDSVASSTAFPSTHSAPPPSCSTAFLHLGDLPAEPSKVIASSSHPDLLGGWDTWADTATPGPASIPVPEGTLFSSAGHPAPPGPNPSQTKSQNLDPFADLSDLSSSLQGLPAGLPAGGFVGAPAPTQKSNSPWQANRPTAPGTSWTPQAKPAPRASEQLRSHFSVIGAREERGVRVPSFAQKPKVSENDFEDLLPNQGFSKSDKKGPKTMAEMRKQELARDTDPLKLKLLDWIEGKERNIRALLSTLHTVLWDGESRWTPVSMADLVTPEQVKKQYRRAVLVVHPDKATGQPYEQYAKMIFMELNDAWSEFENQGSRPLF

Target ORF information:

RefSeq Version	NM_001282051.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus cyclin G associated kinase (Gak), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001282051.1

ORF Insert Sequence:

ATGCACAGGC AGAAACCACC CATCATCCAC AGAGATCTCA AGGTTGAAAA CTTACTGCTT 
AGTAACCAGG GGACCATTAA GCTGTGTGAC TTTGGCAGTG CCACAACCAT CTCCCATTAT 
CCTGACTACA GCTGGAGTGC CCAGAAGCGA GCCATGGTGG AGGAAGAGAT CACAAGGAAC 
ACCACACCCA TGTACAGAAC ACCAGAAATT GTAGACCTGT ATTCCAACTT CCCCATTGGC 
GAAAAGCAGG ATATCTGGGC ACTGGGCTGT ATCTTATACC TGCTGTGTTT CCGGCAGCAT 
CCTTTTGAGG ATGGAGCAAA ACTTCGGATA GTCAATGGGA AGTATTCCAT TCCTGTGAAT 
GACACTCGTT ACACAGTCTT CCATGACCTT ATTCGTGCCA TGCTAAAGGT CAACCCAGAG 
GAGAGGCTGT CCATTGCTGA AGTGGTCCGA CAACTGCAGG AAATTGCAGC AGCTAGGAAT 
GTGAACCCCA AAGCTCCCAT CACAGAGCTG CTGGAGCAGA ATGGTGGCTA TGGGAACTCA 
GGGCCTTCCC GAGCACAACC ACCTTGCGGG GGCACCGTGA ACAGCAGTGG AGTTTTGGCT 
CTGGCAGAGT ATGACCAGCC CTACGGTGGG TTTCTTGATA TTCTGCGGGG TGGGACCGAA 
CGGCTCTTCA CCAACCTCAA GGATACTTCC TCCAAGGTCA TCCAGTCTGT GGCTAACTAT 
GCAAAGGGTG ATCTGGACAT ATCTTACATC ACATCCAGGA TTGCAGTGAT GTCATTCCCA 
GCAGAAGGTG TGGAGTCTGC AATCAAAAAC AATATAGAGG ATGTGCGAAT GTTTCTGGAT 
GCTAAGCATC CAGGACATTA TGCTGTCTAC AACCTTTCTC CAAGGATATA CCGGGCTTCC 
AAGTTTCACA ACCGGGTCAC TGAGTGTGGC TGGGCAGTCA GACGGGCACC ACATCTCCAC 
AGTTTGTATA CTCTATGTAG GAGCATGCAT GCCTGGCTCC GGGAAGACCA CAGGAACGTC 
TGTGTTGTGC ATTGCATGGA TGGGAGAGCT GCATCTGCTG TGGCAGTCTG TGCATTCCTG 
TGCTTCTGCC GTCTCTTCAG CACTGCAGAG GCTGCTGTGT ACATGTTCAG CATGAAGCGC 
TGCCCACCAG GCATTTGGCC ATCCCACAAA AGGTACATTG AATATGTATG TGACATGGTG 
GCAGAGGAGC CCATCACGCC CCATAGCAAG CCAATGCTGG TGAAATCTGT TGTCATGACC 
CCCGTGCCAC TGTTCAGCAA GCAGAGGAAT GGCTGCCGAC CGTTCTGTGA GGTCTACGTT 
GGAGAGGAGC GTGTCACCAC CACGTCGCAG GAATATGACA GAATGAAGGA ATTTAAAATT 
GAGGATGGCA AGGCTGTCAT CCCCCTGGGC GTAACAGTTC AAGGAGACGT GCTCATCATC 
ATCTACCATG CCAGGGCCAC ACTGGGAGGG AGGCTGCAGG CTAAGATGGC GTCCATGAAA 
ATGTTCCAGA TCCAGTTCCA CACTGGGTTC GTGCCTCGAA ACGCAACCAC TGTGAAATTT 
GCAAAATATG ACCTGGATGC TTGTGATATT CAAGAGAAGT ACCCAGATCT GTTCCAGGTG 
AACCTGGAAG TGGAGGTAGA GCCTAGAGAC AGGCCCAGCC GAGAAGCTCC ACCTTGGGAG 
AACACCAGCC TAAGGGGGTT AAACCCCAAG ATCCTCTTTT CCAACAGGGA AGAGCAGCAG 
GACATTCTGT CTAAGTTTGG GAAGCCGGAG CTACCCCGGC AGCCGGGCTC CACAGCTCAG 
TATGATGCTG AGGCAGGGTC TCCGGAGGCT GAGATCACAG AGTCGGACTC ACCGCAGAGC 
AGCAGTACGG ACACCAACCA CTTTCTTCAC ACACTGGATT GGCAGGAGGA AAAAGAACCA 
GAGACTGGGT TAGACAATAC CTCTCCTAAG GAGAGTCAGT CTGTCCTGAT TGCAGACGGA 
GATGGAAGTG AAGTATCAGA TGAAGAAGAG GCTTCATTCC CCAGTGAGGA GAGGAAACCA 
GGAGCTGGAG AAGATACACC AAGGCTGGCT GCTGGGACCA AACAGCAAGA CTTAATATTT 
GATGTGGGCA TGCTGGCTGC CCCACAGGAG CCTGTACAGC CCGAAGAAGG TGTTGATCTC 
CTGGGGCTAC ACTCTGAGGG GGACTTAAGG CCTGCTGCCC CCTTACAGGC TTGTGGGGTC 
CCATCTAGCA ACACTGACCT GTTGAGCTGC CTTCTTGAAC CATCTGATGC TGCTCAAGTA 
GGGCCTCCTG GTGACCTGCT TGGTGGTGAG GCTCCACTGC TGCTAGCAAG CCCAGTTTCT 
CCTCTTGGGC TGCAGAACAA CCTACAAGGA AAAGTCCCTG ACACTGTGGA CCCATTTGAC 
CAGTTCCTGC TGTCATCCAA CTCAGACACC CAGCCCTGCT CCAAGCCTGA TCTCTTTGGA 
GAGTTTCTCA ACTCTGACTC TGTAGCTTCC TCAACTGCCT TCCCATCCAC CCACAGTGCC 
CCACCCCCAT CCTGTAGCAC TGCCTTCCTG CACCTAGGGG ATCTGCCAGC AGAGCCCAGC 
AAGGTAATAG CTTCATCCAG CCACCCAGAT CTGCTAGGAG GATGGGATAC GTGGGCTGAC 
ACAGCTACAC CTGGGCCAGC CTCCATTCCA GTACCAGAAG GTACCCTCTT CTCTTCTGCA 
GGTCACCCAG CCCCTCCTGG CCCCAACCCC AGCCAAACCA AGTCTCAGAA CCTTGACCCA 
TTTGCTGATC TCAGTGATCT CAGCTCTAGC CTCCAAGGCC TGCCTGCTGG ACTTCCTGCA 
GGGGGCTTTG TTGGCGCACC AGCCCCCACT CAAAAGAGCA ACAGCCCCTG GCAGGCAAAT 
CGTCCCACAG CCCCTGGAAC CTCATGGACC CCCCAGGCCA AGCCAGCCCC CAGAGCCTCT 
GAACAGCTAA GGTCTCACTT CAGTGTGATC GGGGCCCGAG AAGAGAGGGG CGTCCGTGTG 
CCCAGCTTTG CCCAAAAGCC AAAGGTCTCA GAAAATGATT TTGAAGATCT TCTGCCTAAT 
CAAGGCTTCT CTAAGTCTGA CAAGAAGGGG CCAAAGACCA TGGCAGAGAT GCGGAAACAG 
GAACTTGCCA GAGATACAGA CCCACTCAAA TTGAAGCTTT TGGACTGGAT TGAAGGCAAG 
GAGAGGAACA TTCGTGCACT GCTGTCCACT CTGCACACGG TATTGTGGGA TGGGGAGAGC 
CGCTGGACAC CTGTGAGTAT GGCTGACCTG GTGACTCCAG AGCAGGTGAA GAAGCAGTAC 
CGCCGTGCAG TGCTGGTAGT GCATCCTGAT AAGGCCACAG GGCAGCCGTA TGAACAGTAT 
GCCAAGATGA TCTTCATGGA GCTGAATGAT GCATGGTCTG AGTTTGAAAA CCAGGGCTCG 
AGGCCCCTCT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gak ( XM_006534907.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006534970.1 Mus musculus cyclin G associated kinase (Gak), transcript variant X3, mRNA.

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CloneID	OMu21899
Clone ID Related Accession (Same CDS sequence)	XM_006534907.2 , XM_006534907.3 , NM_001359924.1 , NM_001282051.1
Accession Version	XM_006534907.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3435bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-08
Organism	Mus musculus(House mouse)
Product	cyclin-G-associated kinase isoform X1
Comment

ATGCACAGGC AGAAACCACC CATCATCCAC AGAGATCTCA AGGTTGAAAA CTTACTGCTT 
AGTAACCAGG GGACCATTAA GCTGTGTGAC TTTGGCAGTG CCACAACCAT CTCCCATTAT 
CCTGACTACA GCTGGAGTGC CCAGAAGCGA GCCATGGTGG AGGAAGAGAT CACAAGGAAC 
ACCACACCCA TGTACAGAAC ACCAGAAATT GTAGACCTGT ATTCCAACTT CCCCATTGGC 
GAAAAGCAGG ATATCTGGGC ACTGGGCTGT ATCTTATACC TGCTGTGTTT CCGGCAGCAT 
CCTTTTGAGG ATGGAGCAAA ACTTCGGATA GTCAATGGGA AGTATTCCAT TCCTGTGAAT 
GACACTCGTT ACACAGTCTT CCATGACCTT ATTCGTGCCA TGCTAAAGGT CAACCCAGAG 
GAGAGGCTGT CCATTGCTGA AGTGGTCCGA CAACTGCAGG AAATTGCAGC AGCTAGGAAT 
GTGAACCCCA AAGCTCCCAT CACAGAGCTG CTGGAGCAGA ATGGTGGCTA TGGGAACTCA 
GGGCCTTCCC GAGCACAACC ACCTTGCGGG GGCACCGTGA ACAGCAGTGG AGTTTTGGCT 
CTGGCAGAGT ATGACCAGCC CTACGGTGGG TTTCTTGATA TTCTGCGGGG TGGGACCGAA 
CGGCTCTTCA CCAACCTCAA GGATACTTCC TCCAAGGTCA TCCAGTCTGT GGCTAACTAT 
GCAAAGGGTG ATCTGGACAT ATCTTACATC ACATCCAGGA TTGCAGTGAT GTCATTCCCA 
GCAGAAGGTG TGGAGTCTGC AATCAAAAAC AATATAGAGG ATGTGCGAAT GTTTCTGGAT 
GCTAAGCATC CAGGACATTA TGCTGTCTAC AACCTTTCTC CAAGGATATA CCGGGCTTCC 
AAGTTTCACA ACCGGGTCAC TGAGTGTGGC TGGGCAGTCA GACGGGCACC ACATCTCCAC 
AGTTTGTATA CTCTATGTAG GAGCATGCAT GCCTGGCTCC GGGAAGACCA CAGGAACGTC 
TGTGTTGTGC ATTGCATGGA TGGGAGAGCT GCATCTGCTG TGGCAGTCTG TGCATTCCTG 
TGCTTCTGCC GTCTCTTCAG CACTGCAGAG GCTGCTGTGT ACATGTTCAG CATGAAGCGC 
TGCCCACCAG GCATTTGGCC ATCCCACAAA AGGTACATTG AATATGTATG TGACATGGTG 
GCAGAGGAGC CCATCACGCC CCATAGCAAG CCAATGCTGG TGAAATCTGT TGTCATGACC 
CCCGTGCCAC TGTTCAGCAA GCAGAGGAAT GGCTGCCGAC CGTTCTGTGA GGTCTACGTT 
GGAGAGGAGC GTGTCACCAC CACGTCGCAG GAATATGACA GAATGAAGGA ATTTAAAATT 
GAGGATGGCA AGGCTGTCAT CCCCCTGGGC GTAACAGTTC AAGGAGACGT GCTCATCATC 
ATCTACCATG CCAGGGCCAC ACTGGGAGGG AGGCTGCAGG CTAAGATGGC GTCCATGAAA 
ATGTTCCAGA TCCAGTTCCA CACTGGGTTC GTGCCTCGAA ACGCAACCAC TGTGAAATTT 
GCAAAATATG ACCTGGATGC TTGTGATATT CAAGAGAAGT ACCCAGATCT GTTCCAGGTG 
AACCTGGAAG TGGAGGTAGA GCCTAGAGAC AGGCCCAGCC GAGAAGCTCC ACCTTGGGAG 
AACACCAGCC TAAGGGGGTT AAACCCCAAG ATCCTCTTTT CCAACAGGGA AGAGCAGCAG 
GACATTCTGT CTAAGTTTGG GAAGCCGGAG CTACCCCGGC AGCCGGGCTC CACAGCTCAG 
TATGATGCTG AGGCAGGGTC TCCGGAGGCT GAGATCACAG AGTCGGACTC ACCGCAGAGC 
AGCAGTACGG ACACCAACCA CTTTCTTCAC ACACTGGATT GGCAGGAGGA AAAAGAACCA 
GAGACTGGGT TAGACAATAC CTCTCCTAAG GAGAGTCAGT CTGTCCTGAT TGCAGACGGA 
GATGGAAGTG AAGTATCAGA TGAAGAAGAG GCTTCATTCC CCAGTGAGGA GAGGAAACCA 
GGAGCTGGAG AAGATACACC AAGGCTGGCT GCTGGGACCA AACAGCAAGA CTTAATATTT 
GATGTGGGCA TGCTGGCTGC CCCACAGGAG CCTGTACAGC CCGAAGAAGG TGTTGATCTC 
CTGGGGCTAC ACTCTGAGGG GGACTTAAGG CCTGCTGCCC CCTTACAGGC TTGTGGGGTC 
CCATCTAGCA ACACTGACCT GTTGAGCTGC CTTCTTGAAC CATCTGATGC TGCTCAAGTA 
GGGCCTCCTG GTGACCTGCT TGGTGGTGAG GCTCCACTGC TGCTAGCAAG CCCAGTTTCT 
CCTCTTGGGC TGCAGAACAA CCTACAAGGA AAAGTCCCTG ACACTGTGGA CCCATTTGAC 
CAGTTCCTGC TGTCATCCAA CTCAGACACC CAGCCCTGCT CCAAGCCTGA TCTCTTTGGA 
GAGTTTCTCA ACTCTGACTC TGTAGCTTCC TCAACTGCCT TCCCATCCAC CCACAGTGCC 
CCACCCCCAT CCTGTAGCAC TGCCTTCCTG CACCTAGGGG ATCTGCCAGC AGAGCCCAGC 
AAGGTAATAG CTTCATCCAG CCACCCAGAT CTGCTAGGAG GATGGGATAC GTGGGCTGAC 
ACAGCTACAC CTGGGCCAGC CTCCATTCCA GTACCAGAAG GTACCCTCTT CTCTTCTGCA 
GGTCACCCAG CCCCTCCTGG CCCCAACCCC AGCCAAACCA AGTCTCAGAA CCTTGACCCA 
TTTGCTGATC TCAGTGATCT CAGCTCTAGC CTCCAAGGCC TGCCTGCTGG ACTTCCTGCA 
GGGGGCTTTG TTGGCGCACC AGCCCCCACT CAAAAGAGCA ACAGCCCCTG GCAGGCAAAT 
CGTCCCACAG CCCCTGGAAC CTCATGGACC CCCCAGGCCA AGCCAGCCCC CAGAGCCTCT 
GAACAGCTAA GGTCTCACTT CAGTGTGATC GGGGCCCGAG AAGAGAGGGG CGTCCGTGTG 
CCCAGCTTTG CCCAAAAGCC AAAGGTCTCA GAAAATGATT TTGAAGATCT TCTGCCTAAT 
CAAGGCTTCT CTAAGTCTGA CAAGAAGGGG CCAAAGACCA TGGCAGAGAT GCGGAAACAG 
GAACTTGCCA GAGATACAGA CCCACTCAAA TTGAAGCTTT TGGACTGGAT TGAAGGCAAG 
GAGAGGAACA TTCGTGCACT GCTGTCCACT CTGCACACGG TATTGTGGGA TGGGGAGAGC 
CGCTGGACAC CTGTGAGTAT GGCTGACCTG GTGACTCCAG AGCAGGTGAA GAAGCAGTAC 
CGCCGTGCAG TGCTGGTAGT GCATCCTGAT AAGGCCACAG GGCAGCCGTA TGAACAGTAT 
GCCAAGATGA TCTTCATGGA GCTGAATGAT GCATGGTCTG AGTTTGAAAA CCAGGGCTCG 
AGGCCCCTCT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_006534970.1

Translation

MHRQKPPIIHRDLKVENLLLSNQGTIKLCDFGSATTISHYPDYSWSAQKRAMVEEEITRNTTPMYRTPEIVDLYSNFPIGEKQDIWALGCILYLLCFRQHPFEDGAKLRIVNGKYSIPVNDTRYTVFHDLIRAMLKVNPEERLSIAEVVRQLQEIAAARNVNPKAPITELLEQNGGYGNSGPSRAQPPCGGTVNSSGVLALAEYDQPYGGFLDILRGGTERLFTNLKDTSSKVIQSVANYAKGDLDISYITSRIAVMSFPAEGVESAIKNNIEDVRMFLDAKHPGHYAVYNLSPRIYRASKFHNRVTECGWAVRRAPHLHSLYTLCRSMHAWLREDHRNVCVVHCMDGRAASAVAVCAFLCFCRLFSTAEAAVYMFSMKRCPPGIWPSHKRYIEYVCDMVAEEPITPHSKPMLVKSVVMTPVPLFSKQRNGCRPFCEVYVGEERVTTTSQEYDRMKEFKIEDGKAVIPLGVTVQGDVLIIIYHARATLGGRLQAKMASMKMFQIQFHTGFVPRNATTVKFAKYDLDACDIQEKYPDLFQVNLEVEVEPRDRPSREAPPWENTSLRGLNPKILFSNREEQQDILSKFGKPELPRQPGSTAQYDAEAGSPEAEITESDSPQSSSTDTNHFLHTLDWQEEKEPETGLDNTSPKESQSVLIADGDGSEVSDEEEASFPSEERKPGAGEDTPRLAAGTKQQDLIFDVGMLAAPQEPVQPEEGVDLLGLHSEGDLRPAAPLQACGVPSSNTDLLSCLLEPSDAAQVGPPGDLLGGEAPLLLASPVSPLGLQNNLQGKVPDTVDPFDQFLLSSNSDTQPCSKPDLFGEFLNSDSVASSTAFPSTHSAPPPSCSTAFLHLGDLPAEPSKVIASSSHPDLLGGWDTWADTATPGPASIPVPEGTLFSSAGHPAPPGPNPSQTKSQNLDPFADLSDLSSSLQGLPAGLPAGGFVGAPAPTQKSNSPWQANRPTAPGTSWTPQAKPAPRASEQLRSHFSVIGAREERGVRVPSFAQKPKVSENDFEDLLPNQGFSKSDKKGPKTMAEMRKQELARDTDPLKLKLLDWIEGKERNIRALLSTLHTVLWDGESRWTPVSMADLVTPEQVKKQYRRAVLVVHPDKATGQPYEQYAKMIFMELNDAWSEFENQGSRPLF

Target ORF information:

RefSeq Version	XM_006534907.2
Organism	Mus musculus(House mouse)
Definition	Mus musculus cyclin G associated kinase (Gak), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006534907.2

ORF Insert Sequence:

ATGCACAGGC AGAAACCACC CATCATCCAC AGAGATCTCA AGGTTGAAAA CTTACTGCTT 
AGTAACCAGG GGACCATTAA GCTGTGTGAC TTTGGCAGTG CCACAACCAT CTCCCATTAT 
CCTGACTACA GCTGGAGTGC CCAGAAGCGA GCCATGGTGG AGGAAGAGAT CACAAGGAAC 
ACCACACCCA TGTACAGAAC ACCAGAAATT GTAGACCTGT ATTCCAACTT CCCCATTGGC 
GAAAAGCAGG ATATCTGGGC ACTGGGCTGT ATCTTATACC TGCTGTGTTT CCGGCAGCAT 
CCTTTTGAGG ATGGAGCAAA ACTTCGGATA GTCAATGGGA AGTATTCCAT TCCTGTGAAT 
GACACTCGTT ACACAGTCTT CCATGACCTT ATTCGTGCCA TGCTAAAGGT CAACCCAGAG 
GAGAGGCTGT CCATTGCTGA AGTGGTCCGA CAACTGCAGG AAATTGCAGC AGCTAGGAAT 
GTGAACCCCA AAGCTCCCAT CACAGAGCTG CTGGAGCAGA ATGGTGGCTA TGGGAACTCA 
GGGCCTTCCC GAGCACAACC ACCTTGCGGG GGCACCGTGA ACAGCAGTGG AGTTTTGGCT 
CTGGCAGAGT ATGACCAGCC CTACGGTGGG TTTCTTGATA TTCTGCGGGG TGGGACCGAA 
CGGCTCTTCA CCAACCTCAA GGATACTTCC TCCAAGGTCA TCCAGTCTGT GGCTAACTAT 
GCAAAGGGTG ATCTGGACAT ATCTTACATC ACATCCAGGA TTGCAGTGAT GTCATTCCCA 
GCAGAAGGTG TGGAGTCTGC AATCAAAAAC AATATAGAGG ATGTGCGAAT GTTTCTGGAT 
GCTAAGCATC CAGGACATTA TGCTGTCTAC AACCTTTCTC CAAGGATATA CCGGGCTTCC 
AAGTTTCACA ACCGGGTCAC TGAGTGTGGC TGGGCAGTCA GACGGGCACC ACATCTCCAC 
AGTTTGTATA CTCTATGTAG GAGCATGCAT GCCTGGCTCC GGGAAGACCA CAGGAACGTC 
TGTGTTGTGC ATTGCATGGA TGGGAGAGCT GCATCTGCTG TGGCAGTCTG TGCATTCCTG 
TGCTTCTGCC GTCTCTTCAG CACTGCAGAG GCTGCTGTGT ACATGTTCAG CATGAAGCGC 
TGCCCACCAG GCATTTGGCC ATCCCACAAA AGGTACATTG AATATGTATG TGACATGGTG 
GCAGAGGAGC CCATCACGCC CCATAGCAAG CCAATGCTGG TGAAATCTGT TGTCATGACC 
CCCGTGCCAC TGTTCAGCAA GCAGAGGAAT GGCTGCCGAC CGTTCTGTGA GGTCTACGTT 
GGAGAGGAGC GTGTCACCAC CACGTCGCAG GAATATGACA GAATGAAGGA ATTTAAAATT 
GAGGATGGCA AGGCTGTCAT CCCCCTGGGC GTAACAGTTC AAGGAGACGT GCTCATCATC 
ATCTACCATG CCAGGGCCAC ACTGGGAGGG AGGCTGCAGG CTAAGATGGC GTCCATGAAA 
ATGTTCCAGA TCCAGTTCCA CACTGGGTTC GTGCCTCGAA ACGCAACCAC TGTGAAATTT 
GCAAAATATG ACCTGGATGC TTGTGATATT CAAGAGAAGT ACCCAGATCT GTTCCAGGTG 
AACCTGGAAG TGGAGGTAGA GCCTAGAGAC AGGCCCAGCC GAGAAGCTCC ACCTTGGGAG 
AACACCAGCC TAAGGGGGTT AAACCCCAAG ATCCTCTTTT CCAACAGGGA AGAGCAGCAG 
GACATTCTGT CTAAGTTTGG GAAGCCGGAG CTACCCCGGC AGCCGGGCTC CACAGCTCAG 
TATGATGCTG AGGCAGGGTC TCCGGAGGCT GAGATCACAG AGTCGGACTC ACCGCAGAGC 
AGCAGTACGG ACACCAACCA CTTTCTTCAC ACACTGGATT GGCAGGAGGA AAAAGAACCA 
GAGACTGGGT TAGACAATAC CTCTCCTAAG GAGAGTCAGT CTGTCCTGAT TGCAGACGGA 
GATGGAAGTG AAGTATCAGA TGAAGAAGAG GCTTCATTCC CCAGTGAGGA GAGGAAACCA 
GGAGCTGGAG AAGATACACC AAGGCTGGCT GCTGGGACCA AACAGCAAGA CTTAATATTT 
GATGTGGGCA TGCTGGCTGC CCCACAGGAG CCTGTACAGC CCGAAGAAGG TGTTGATCTC 
CTGGGGCTAC ACTCTGAGGG GGACTTAAGG CCTGCTGCCC CCTTACAGGC TTGTGGGGTC 
CCATCTAGCA ACACTGACCT GTTGAGCTGC CTTCTTGAAC CATCTGATGC TGCTCAAGTA 
GGGCCTCCTG GTGACCTGCT TGGTGGTGAG GCTCCACTGC TGCTAGCAAG CCCAGTTTCT 
CCTCTTGGGC TGCAGAACAA CCTACAAGGA AAAGTCCCTG ACACTGTGGA CCCATTTGAC 
CAGTTCCTGC TGTCATCCAA CTCAGACACC CAGCCCTGCT CCAAGCCTGA TCTCTTTGGA 
GAGTTTCTCA ACTCTGACTC TGTAGCTTCC TCAACTGCCT TCCCATCCAC CCACAGTGCC 
CCACCCCCAT CCTGTAGCAC TGCCTTCCTG CACCTAGGGG ATCTGCCAGC AGAGCCCAGC 
AAGGTAATAG CTTCATCCAG CCACCCAGAT CTGCTAGGAG GATGGGATAC GTGGGCTGAC 
ACAGCTACAC CTGGGCCAGC CTCCATTCCA GTACCAGAAG GTACCCTCTT CTCTTCTGCA 
GGTCACCCAG CCCCTCCTGG CCCCAACCCC AGCCAAACCA AGTCTCAGAA CCTTGACCCA 
TTTGCTGATC TCAGTGATCT CAGCTCTAGC CTCCAAGGCC TGCCTGCTGG ACTTCCTGCA 
GGGGGCTTTG TTGGCGCACC AGCCCCCACT CAAAAGAGCA ACAGCCCCTG GCAGGCAAAT 
CGTCCCACAG CCCCTGGAAC CTCATGGACC CCCCAGGCCA AGCCAGCCCC CAGAGCCTCT 
GAACAGCTAA GGTCTCACTT CAGTGTGATC GGGGCCCGAG AAGAGAGGGG CGTCCGTGTG 
CCCAGCTTTG CCCAAAAGCC AAAGGTCTCA GAAAATGATT TTGAAGATCT TCTGCCTAAT 
CAAGGCTTCT CTAAGTCTGA CAAGAAGGGG CCAAAGACCA TGGCAGAGAT GCGGAAACAG 
GAACTTGCCA GAGATACAGA CCCACTCAAA TTGAAGCTTT TGGACTGGAT TGAAGGCAAG 
GAGAGGAACA TTCGTGCACT GCTGTCCACT CTGCACACGG TATTGTGGGA TGGGGAGAGC 
CGCTGGACAC CTGTGAGTAT GGCTGACCTG GTGACTCCAG AGCAGGTGAA GAAGCAGTAC 
CGCCGTGCAG TGCTGGTAGT GCATCCTGAT AAGGCCACAG GGCAGCCGTA TGAACAGTAT 
GCCAAGATGA TCTTCATGGA GCTGAATGAT GCATGGTCTG AGTTTGAAAA CCAGGGCTCG 
AGGCCCCTCT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gak ( XM_006534907.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006534970.1 Mus musculus cyclin G associated kinase (Gak), transcript variant X3, mRNA.

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CloneID	OMu21899
Clone ID Related Accession (Same CDS sequence)	XM_006534907.2 , XM_006534907.3 , NM_001359924.1 , NM_001282051.1
Accession Version	XM_006534907.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3435bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	cyclin-G-associated kinase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006534907.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACAGGC AGAAACCACC CATCATCCAC AGAGATCTCA AGGTTGAAAA CTTACTGCTT 
AGTAACCAGG GGACCATTAA GCTGTGTGAC TTTGGCAGTG CCACAACCAT CTCCCATTAT 
CCTGACTACA GCTGGAGTGC CCAGAAGCGA GCCATGGTGG AGGAAGAGAT CACAAGGAAC 
ACCACACCCA TGTACAGAAC ACCAGAAATT GTAGACCTGT ATTCCAACTT CCCCATTGGC 
GAAAAGCAGG ATATCTGGGC ACTGGGCTGT ATCTTATACC TGCTGTGTTT CCGGCAGCAT 
CCTTTTGAGG ATGGAGCAAA ACTTCGGATA GTCAATGGGA AGTATTCCAT TCCTGTGAAT 
GACACTCGTT ACACAGTCTT CCATGACCTT ATTCGTGCCA TGCTAAAGGT CAACCCAGAG 
GAGAGGCTGT CCATTGCTGA AGTGGTCCGA CAACTGCAGG AAATTGCAGC AGCTAGGAAT 
GTGAACCCCA AAGCTCCCAT CACAGAGCTG CTGGAGCAGA ATGGTGGCTA TGGGAACTCA 
GGGCCTTCCC GAGCACAACC ACCTTGCGGG GGCACCGTGA ACAGCAGTGG AGTTTTGGCT 
CTGGCAGAGT ATGACCAGCC CTACGGTGGG TTTCTTGATA TTCTGCGGGG TGGGACCGAA 
CGGCTCTTCA CCAACCTCAA GGATACTTCC TCCAAGGTCA TCCAGTCTGT GGCTAACTAT 
GCAAAGGGTG ATCTGGACAT ATCTTACATC ACATCCAGGA TTGCAGTGAT GTCATTCCCA 
GCAGAAGGTG TGGAGTCTGC AATCAAAAAC AATATAGAGG ATGTGCGAAT GTTTCTGGAT 
GCTAAGCATC CAGGACATTA TGCTGTCTAC AACCTTTCTC CAAGGATATA CCGGGCTTCC 
AAGTTTCACA ACCGGGTCAC TGAGTGTGGC TGGGCAGTCA GACGGGCACC ACATCTCCAC 
AGTTTGTATA CTCTATGTAG GAGCATGCAT GCCTGGCTCC GGGAAGACCA CAGGAACGTC 
TGTGTTGTGC ATTGCATGGA TGGGAGAGCT GCATCTGCTG TGGCAGTCTG TGCATTCCTG 
TGCTTCTGCC GTCTCTTCAG CACTGCAGAG GCTGCTGTGT ACATGTTCAG CATGAAGCGC 
TGCCCACCAG GCATTTGGCC ATCCCACAAA AGGTACATTG AATATGTATG TGACATGGTG 
GCAGAGGAGC CCATCACGCC CCATAGCAAG CCAATGCTGG TGAAATCTGT TGTCATGACC 
CCCGTGCCAC TGTTCAGCAA GCAGAGGAAT GGCTGCCGAC CGTTCTGTGA GGTCTACGTT 
GGAGAGGAGC GTGTCACCAC CACGTCGCAG GAATATGACA GAATGAAGGA ATTTAAAATT 
GAGGATGGCA AGGCTGTCAT CCCCCTGGGC GTAACAGTTC AAGGAGACGT GCTCATCATC 
ATCTACCATG CCAGGGCCAC ACTGGGAGGG AGGCTGCAGG CTAAGATGGC GTCCATGAAA 
ATGTTCCAGA TCCAGTTCCA CACTGGGTTC GTGCCTCGAA ACGCAACCAC TGTGAAATTT 
GCAAAATATG ACCTGGATGC TTGTGATATT CAAGAGAAGT ACCCAGATCT GTTCCAGGTG 
AACCTGGAAG TGGAGGTAGA GCCTAGAGAC AGGCCCAGCC GAGAAGCTCC ACCTTGGGAG 
AACACCAGCC TAAGGGGGTT AAACCCCAAG ATCCTCTTTT CCAACAGGGA AGAGCAGCAG 
GACATTCTGT CTAAGTTTGG GAAGCCGGAG CTACCCCGGC AGCCGGGCTC CACAGCTCAG 
TATGATGCTG AGGCAGGGTC TCCGGAGGCT GAGATCACAG AGTCGGACTC ACCGCAGAGC 
AGCAGTACGG ACACCAACCA CTTTCTTCAC ACACTGGATT GGCAGGAGGA AAAAGAACCA 
GAGACTGGGT TAGACAATAC CTCTCCTAAG GAGAGTCAGT CTGTCCTGAT TGCAGACGGA 
GATGGAAGTG AAGTATCAGA TGAAGAAGAG GCTTCATTCC CCAGTGAGGA GAGGAAACCA 
GGAGCTGGAG AAGATACACC AAGGCTGGCT GCTGGGACCA AACAGCAAGA CTTAATATTT 
GATGTGGGCA TGCTGGCTGC CCCACAGGAG CCTGTACAGC CCGAAGAAGG TGTTGATCTC 
CTGGGGCTAC ACTCTGAGGG GGACTTAAGG CCTGCTGCCC CCTTACAGGC TTGTGGGGTC 
CCATCTAGCA ACACTGACCT GTTGAGCTGC CTTCTTGAAC CATCTGATGC TGCTCAAGTA 
GGGCCTCCTG GTGACCTGCT TGGTGGTGAG GCTCCACTGC TGCTAGCAAG CCCAGTTTCT 
CCTCTTGGGC TGCAGAACAA CCTACAAGGA AAAGTCCCTG ACACTGTGGA CCCATTTGAC 
CAGTTCCTGC TGTCATCCAA CTCAGACACC CAGCCCTGCT CCAAGCCTGA TCTCTTTGGA 
GAGTTTCTCA ACTCTGACTC TGTAGCTTCC TCAACTGCCT TCCCATCCAC CCACAGTGCC 
CCACCCCCAT CCTGTAGCAC TGCCTTCCTG CACCTAGGGG ATCTGCCAGC AGAGCCCAGC 
AAGGTAATAG CTTCATCCAG CCACCCAGAT CTGCTAGGAG GATGGGATAC GTGGGCTGAC 
ACAGCTACAC CTGGGCCAGC CTCCATTCCA GTACCAGAAG GTACCCTCTT CTCTTCTGCA 
GGTCACCCAG CCCCTCCTGG CCCCAACCCC AGCCAAACCA AGTCTCAGAA CCTTGACCCA 
TTTGCTGATC TCAGTGATCT CAGCTCTAGC CTCCAAGGCC TGCCTGCTGG ACTTCCTGCA 
GGGGGCTTTG TTGGCGCACC AGCCCCCACT CAAAAGAGCA ACAGCCCCTG GCAGGCAAAT 
CGTCCCACAG CCCCTGGAAC CTCATGGACC CCCCAGGCCA AGCCAGCCCC CAGAGCCTCT 
GAACAGCTAA GGTCTCACTT CAGTGTGATC GGGGCCCGAG AAGAGAGGGG CGTCCGTGTG 
CCCAGCTTTG CCCAAAAGCC AAAGGTCTCA GAAAATGATT TTGAAGATCT TCTGCCTAAT 
CAAGGCTTCT CTAAGTCTGA CAAGAAGGGG CCAAAGACCA TGGCAGAGAT GCGGAAACAG 
GAACTTGCCA GAGATACAGA CCCACTCAAA TTGAAGCTTT TGGACTGGAT TGAAGGCAAG 
GAGAGGAACA TTCGTGCACT GCTGTCCACT CTGCACACGG TATTGTGGGA TGGGGAGAGC 
CGCTGGACAC CTGTGAGTAT GGCTGACCTG GTGACTCCAG AGCAGGTGAA GAAGCAGTAC 
CGCCGTGCAG TGCTGGTAGT GCATCCTGAT AAGGCCACAG GGCAGCCGTA TGAACAGTAT 
GCCAAGATGA TCTTCATGGA GCTGAATGAT GCATGGTCTG AGTTTGAAAA CCAGGGCTCG 
AGGCCCCTCT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006534970.1
CDS	419..3853
Translation	MHRQKPPIIHRDLKVENLLLSNQGTIKLCDFGSATTISHYPDYSWSAQKRAMVEEEITRNTTPMYRTPEIVDLYSNFPIGEKQDIWALGCILYLLCFRQHPFEDGAKLRIVNGKYSIPVNDTRYTVFHDLIRAMLKVNPEERLSIAEVVRQLQEIAAARNVNPKAPITELLEQNGGYGNSGPSRAQPPCGGTVNSSGVLALAEYDQPYGGFLDILRGGTERLFTNLKDTSSKVIQSVANYAKGDLDISYITSRIAVMSFPAEGVESAIKNNIEDVRMFLDAKHPGHYAVYNLSPRIYRASKFHNRVTECGWAVRRAPHLHSLYTLCRSMHAWLREDHRNVCVVHCMDGRAASAVAVCAFLCFCRLFSTAEAAVYMFSMKRCPPGIWPSHKRYIEYVCDMVAEEPITPHSKPMLVKSVVMTPVPLFSKQRNGCRPFCEVYVGEERVTTTSQEYDRMKEFKIEDGKAVIPLGVTVQGDVLIIIYHARATLGGRLQAKMASMKMFQIQFHTGFVPRNATTVKFAKYDLDACDIQEKYPDLFQVNLEVEVEPRDRPSREAPPWENTSLRGLNPKILFSNREEQQDILSKFGKPELPRQPGSTAQYDAEAGSPEAEITESDSPQSSSTDTNHFLHTLDWQEEKEPETGLDNTSPKESQSVLIADGDGSEVSDEEEASFPSEERKPGAGEDTPRLAAGTKQQDLIFDVGMLAAPQEPVQPEEGVDLLGLHSEGDLRPAAPLQACGVPSSNTDLLSCLLEPSDAAQVGPPGDLLGGEAPLLLASPVSPLGLQNNLQGKVPDTVDPFDQFLLSSNSDTQPCSKPDLFGEFLNSDSVASSTAFPSTHSAPPPSCSTAFLHLGDLPAEPSKVIASSSHPDLLGGWDTWADTATPGPASIPVPEGTLFSSAGHPAPPGPNPSQTKSQNLDPFADLSDLSSSLQGLPAGLPAGGFVGAPAPTQKSNSPWQANRPTAPGTSWTPQAKPAPRASEQLRSHFSVIGAREERGVRVPSFAQKPKVSENDFEDLLPNQGFSKSDKKGPKTMAEMRKQELARDTDPLKLKLLDWIEGKERNIRALLSTLHTVLWDGESRWTPVSMADLVTPEQVKKQYRRAVLVVHPDKATGQPYEQYAKMIFMELNDAWSEFENQGSRPLF

Target ORF information:

RefSeq Version	XM_006534907.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus cyclin G associated kinase (Gak), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006534907.3

ORF Insert Sequence:

ATGCACAGGC AGAAACCACC CATCATCCAC AGAGATCTCA AGGTTGAAAA CTTACTGCTT 
AGTAACCAGG GGACCATTAA GCTGTGTGAC TTTGGCAGTG CCACAACCAT CTCCCATTAT 
CCTGACTACA GCTGGAGTGC CCAGAAGCGA GCCATGGTGG AGGAAGAGAT CACAAGGAAC 
ACCACACCCA TGTACAGAAC ACCAGAAATT GTAGACCTGT ATTCCAACTT CCCCATTGGC 
GAAAAGCAGG ATATCTGGGC ACTGGGCTGT ATCTTATACC TGCTGTGTTT CCGGCAGCAT 
CCTTTTGAGG ATGGAGCAAA ACTTCGGATA GTCAATGGGA AGTATTCCAT TCCTGTGAAT 
GACACTCGTT ACACAGTCTT CCATGACCTT ATTCGTGCCA TGCTAAAGGT CAACCCAGAG 
GAGAGGCTGT CCATTGCTGA AGTGGTCCGA CAACTGCAGG AAATTGCAGC AGCTAGGAAT 
GTGAACCCCA AAGCTCCCAT CACAGAGCTG CTGGAGCAGA ATGGTGGCTA TGGGAACTCA 
GGGCCTTCCC GAGCACAACC ACCTTGCGGG GGCACCGTGA ACAGCAGTGG AGTTTTGGCT 
CTGGCAGAGT ATGACCAGCC CTACGGTGGG TTTCTTGATA TTCTGCGGGG TGGGACCGAA 
CGGCTCTTCA CCAACCTCAA GGATACTTCC TCCAAGGTCA TCCAGTCTGT GGCTAACTAT 
GCAAAGGGTG ATCTGGACAT ATCTTACATC ACATCCAGGA TTGCAGTGAT GTCATTCCCA 
GCAGAAGGTG TGGAGTCTGC AATCAAAAAC AATATAGAGG ATGTGCGAAT GTTTCTGGAT 
GCTAAGCATC CAGGACATTA TGCTGTCTAC AACCTTTCTC CAAGGATATA CCGGGCTTCC 
AAGTTTCACA ACCGGGTCAC TGAGTGTGGC TGGGCAGTCA GACGGGCACC ACATCTCCAC 
AGTTTGTATA CTCTATGTAG GAGCATGCAT GCCTGGCTCC GGGAAGACCA CAGGAACGTC 
TGTGTTGTGC ATTGCATGGA TGGGAGAGCT GCATCTGCTG TGGCAGTCTG TGCATTCCTG 
TGCTTCTGCC GTCTCTTCAG CACTGCAGAG GCTGCTGTGT ACATGTTCAG CATGAAGCGC 
TGCCCACCAG GCATTTGGCC ATCCCACAAA AGGTACATTG AATATGTATG TGACATGGTG 
GCAGAGGAGC CCATCACGCC CCATAGCAAG CCAATGCTGG TGAAATCTGT TGTCATGACC 
CCCGTGCCAC TGTTCAGCAA GCAGAGGAAT GGCTGCCGAC CGTTCTGTGA GGTCTACGTT 
GGAGAGGAGC GTGTCACCAC CACGTCGCAG GAATATGACA GAATGAAGGA ATTTAAAATT 
GAGGATGGCA AGGCTGTCAT CCCCCTGGGC GTAACAGTTC AAGGAGACGT GCTCATCATC 
ATCTACCATG CCAGGGCCAC ACTGGGAGGG AGGCTGCAGG CTAAGATGGC GTCCATGAAA 
ATGTTCCAGA TCCAGTTCCA CACTGGGTTC GTGCCTCGAA ACGCAACCAC TGTGAAATTT 
GCAAAATATG ACCTGGATGC TTGTGATATT CAAGAGAAGT ACCCAGATCT GTTCCAGGTG 
AACCTGGAAG TGGAGGTAGA GCCTAGAGAC AGGCCCAGCC GAGAAGCTCC ACCTTGGGAG 
AACACCAGCC TAAGGGGGTT AAACCCCAAG ATCCTCTTTT CCAACAGGGA AGAGCAGCAG 
GACATTCTGT CTAAGTTTGG GAAGCCGGAG CTACCCCGGC AGCCGGGCTC CACAGCTCAG 
TATGATGCTG AGGCAGGGTC TCCGGAGGCT GAGATCACAG AGTCGGACTC ACCGCAGAGC 
AGCAGTACGG ACACCAACCA CTTTCTTCAC ACACTGGATT GGCAGGAGGA AAAAGAACCA 
GAGACTGGGT TAGACAATAC CTCTCCTAAG GAGAGTCAGT CTGTCCTGAT TGCAGACGGA 
GATGGAAGTG AAGTATCAGA TGAAGAAGAG GCTTCATTCC CCAGTGAGGA GAGGAAACCA 
GGAGCTGGAG AAGATACACC AAGGCTGGCT GCTGGGACCA AACAGCAAGA CTTAATATTT 
GATGTGGGCA TGCTGGCTGC CCCACAGGAG CCTGTACAGC CCGAAGAAGG TGTTGATCTC 
CTGGGGCTAC ACTCTGAGGG GGACTTAAGG CCTGCTGCCC CCTTACAGGC TTGTGGGGTC 
CCATCTAGCA ACACTGACCT GTTGAGCTGC CTTCTTGAAC CATCTGATGC TGCTCAAGTA 
GGGCCTCCTG GTGACCTGCT TGGTGGTGAG GCTCCACTGC TGCTAGCAAG CCCAGTTTCT 
CCTCTTGGGC TGCAGAACAA CCTACAAGGA AAAGTCCCTG ACACTGTGGA CCCATTTGAC 
CAGTTCCTGC TGTCATCCAA CTCAGACACC CAGCCCTGCT CCAAGCCTGA TCTCTTTGGA 
GAGTTTCTCA ACTCTGACTC TGTAGCTTCC TCAACTGCCT TCCCATCCAC CCACAGTGCC 
CCACCCCCAT CCTGTAGCAC TGCCTTCCTG CACCTAGGGG ATCTGCCAGC AGAGCCCAGC 
AAGGTAATAG CTTCATCCAG CCACCCAGAT CTGCTAGGAG GATGGGATAC GTGGGCTGAC 
ACAGCTACAC CTGGGCCAGC CTCCATTCCA GTACCAGAAG GTACCCTCTT CTCTTCTGCA 
GGTCACCCAG CCCCTCCTGG CCCCAACCCC AGCCAAACCA AGTCTCAGAA CCTTGACCCA 
TTTGCTGATC TCAGTGATCT CAGCTCTAGC CTCCAAGGCC TGCCTGCTGG ACTTCCTGCA 
GGGGGCTTTG TTGGCGCACC AGCCCCCACT CAAAAGAGCA ACAGCCCCTG GCAGGCAAAT 
CGTCCCACAG CCCCTGGAAC CTCATGGACC CCCCAGGCCA AGCCAGCCCC CAGAGCCTCT 
GAACAGCTAA GGTCTCACTT CAGTGTGATC GGGGCCCGAG AAGAGAGGGG CGTCCGTGTG 
CCCAGCTTTG CCCAAAAGCC AAAGGTCTCA GAAAATGATT TTGAAGATCT TCTGCCTAAT 
CAAGGCTTCT CTAAGTCTGA CAAGAAGGGG CCAAAGACCA TGGCAGAGAT GCGGAAACAG 
GAACTTGCCA GAGATACAGA CCCACTCAAA TTGAAGCTTT TGGACTGGAT TGAAGGCAAG 
GAGAGGAACA TTCGTGCACT GCTGTCCACT CTGCACACGG TATTGTGGGA TGGGGAGAGC 
CGCTGGACAC CTGTGAGTAT GGCTGACCTG GTGACTCCAG AGCAGGTGAA GAAGCAGTAC 
CGCCGTGCAG TGCTGGTAGT GCATCCTGAT AAGGCCACAG GGCAGCCGTA TGAACAGTAT 
GCCAAGATGA TCTTCATGGA GCTGAATGAT GCATGGTCTG AGTTTGAAAA CCAGGGCTCG 
AGGCCCCTCT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gak ( NM_001282052.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001268981.1 Mus musculus cyclin G associated kinase (Gak), transcript variant 3, mRNA.

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CloneID	OMu21889
Clone ID Related Accession (Same CDS sequence)	NM_001282052.1
Accession Version	NM_001282052.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3282bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-12
Organism	Mus musculus(house mouse)
Product	cyclin-G-associated kinase isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY312464.1, BC062975.1, AK166682.1, AI466057.1 and AC133896.7. Transcript Variant: This variant (3) differs in its 5' UTR and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (3) has a shorter N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK133581.1, AK161151.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGGAGG AAGAGATCAC AAGGAACACC ACACCCATGT ACAGAACACC AGAAATTGTA 
GACCTGTATT CCAACTTCCC CATTGGCGAA AAGCAGGATA TCTGGGCACT GGGCTGTATC 
TTATACCTGC TGTGTTTCCG GCAGCATCCT TTTGAGGATG GAGCAAAACT TCGGATAGTC 
AATGGGAAGT ATTCCATTCC TGTGAATGAC ACTCGTTACA CAGTCTTCCA TGACCTTATT 
CGTGCCATGC TAAAGGTCAA CCCAGAGGAG AGGCTGTCCA TTGCTGAAGT GGTCCGACAA 
CTGCAGGAAA TTGCAGCAGC TAGGAATGTG AACCCCAAAG CTCCCATCAC AGAGCTGCTG 
GAGCAGAATG GTGGCTATGG GAACTCAGGG CCTTCCCGAG CACAACCACC TTGCGGGGGC 
ACCGTGAACA GCAGTGGAGT TTTGGCTCTG GCAGAGTATG ACCAGCCCTA CGGTGGGTTT 
CTTGATATTC TGCGGGGTGG GACCGAACGG CTCTTCACCA ACCTCAAGGA TACTTCCTCC 
AAGGTCATCC AGTCTGTGGC TAACTATGCA AAGGGTGATC TGGACATATC TTACATCACA 
TCCAGGATTG CAGTGATGTC ATTCCCAGCA GAAGGTGTGG AGTCTGCAAT CAAAAACAAT 
ATAGAGGATG TGCGAATGTT TCTGGATGCT AAGCATCCAG GACATTATGC TGTCTACAAC 
CTTTCTCCAA GGATATACCG GGCTTCCAAG TTTCACAACC GGGTCACTGA GTGTGGCTGG 
GCAGTCAGAC GGGCACCACA TCTCCACAGT TTGTATACTC TATGTAGGAG CATGCATGCC 
TGGCTCCGGG AAGACCACAG GAACGTCTGT GTTGTGCATT GCATGGATGG GAGAGCTGCA 
TCTGCTGTGG CAGTCTGTGC ATTCCTGTGC TTCTGCCGTC TCTTCAGCAC TGCAGAGGCT 
GCTGTGTACA TGTTCAGCAT GAAGCGCTGC CCACCAGGCA TTTGGCCATC CCACAAAAGG 
TACATTGAAT ATGTATGTGA CATGGTGGCA GAGGAGCCCA TCACGCCCCA TAGCAAGCCA 
ATGCTGGTGA AATCTGTTGT CATGACCCCC GTGCCACTGT TCAGCAAGCA GAGGAATGGC 
TGCCGACCGT TCTGTGAGGT CTACGTTGGA GAGGAGCGTG TCACCACCAC GTCGCAGGAA 
TATGACAGAA TGAAGGAATT TAAAATTGAG GATGGCAAGG CTGTCATCCC CCTGGGCGTA 
ACAGTTCAAG GAGACGTGCT CATCATCATC TACCATGCCA GGGCCACACT GGGAGGGAGG 
CTGCAGGCTA AGATGGCGTC CATGAAAATG TTCCAGATCC AGTTCCACAC TGGGTTCGTG 
CCTCGAAACG CAACCACTGT GAAATTTGCA AAATATGACC TGGATGCTTG TGATATTCAA 
GAGAAGTACC CAGATCTGTT CCAGGTGAAC CTGGAAGTGG AGGTAGAGCC TAGAGACAGG 
CCCAGCCGAG AAGCTCCACC TTGGGAGAAC ACCAGCCTAA GGGGGTTAAA CCCCAAGATC 
CTCTTTTCCA ACAGGGAAGA GCAGCAGGAC ATTCTGTCTA AGTTTGGGAA GCCGGAGCTA 
CCCCGGCAGC CGGGCTCCAC AGCTCAGTAT GATGCTGAGG CAGGGTCTCC GGAGGCTGAG 
ATCACAGAGT CGGACTCACC GCAGAGCAGC AGTACGGACA CCAACCACTT TCTTCACACA 
CTGGATTGGC AGGAGGAAAA AGAACCAGAG ACTGGGTTAG ACAATACCTC TCCTAAGGAG 
AGTCAGTCTG TCCTGATTGC AGACGGAGAT GGAAGTGAAG TATCAGATGA AGAAGAGGCT 
TCATTCCCCA GTGAGGAGAG GAAACCAGGA GCTGGAGAAG ATACACCAAG GCTGGCTGCT 
GGGACCAAAC AGCAAGACTT AATATTTGAT GTGGGCATGC TGGCTGCCCC ACAGGAGCCT 
GTACAGCCCG AAGAAGGTGT TGATCTCCTG GGGCTACACT CTGAGGGGGA CTTAAGGCCT 
GCTGCCCCCT TACAGGCTTG TGGGGTCCCA TCTAGCAACA CTGACCTGTT GAGCTGCCTT 
CTTGAACCAT CTGATGCTGC TCAAGTAGGG CCTCCTGGTG ACCTGCTTGG TGGTGAGGCT 
CCACTGCTGC TAGCAAGCCC AGTTTCTCCT CTTGGGCTGC AGAACAACCT ACAAGGAAAA 
GTCCCTGACA CTGTGGACCC ATTTGACCAG TTCCTGCTGT CATCCAACTC AGACACCCAG 
CCCTGCTCCA AGCCTGATCT CTTTGGAGAG TTTCTCAACT CTGACTCTGT AGCTTCCTCA 
ACTGCCTTCC CATCCACCCA CAGTGCCCCA CCCCCATCCT GTAGCACTGC CTTCCTGCAC 
CTAGGGGATC TGCCAGCAGA GCCCAGCAAG GTAATAGCTT CATCCAGCCA CCCAGATCTG 
CTAGGAGGAT GGGATACGTG GGCTGACACA GCTACACCTG GGCCAGCCTC CATTCCAGTA 
CCAGAAGGTA CCCTCTTCTC TTCTGCAGGT CACCCAGCCC CTCCTGGCCC CAACCCCAGC 
CAAACCAAGT CTCAGAACCT TGACCCATTT GCTGATCTCA GTGATCTCAG CTCTAGCCTC 
CAAGGCCTGC CTGCTGGACT TCCTGCAGGG GGCTTTGTTG GCGCACCAGC CCCCACTCAA 
AAGAGCAACA GCCCCTGGCA GGCAAATCGT CCCACAGCCC CTGGAACCTC ATGGACCCCC 
CAGGCCAAGC CAGCCCCCAG AGCCTCTGAA CAGCTAAGGT CTCACTTCAG TGTGATCGGG 
GCCCGAGAAG AGAGGGGCGT CCGTGTGCCC AGCTTTGCCC AAAAGCCAAA GGTCTCAGAA 
AATGATTTTG AAGATCTTCT GCCTAATCAA GGCTTCTCTA AGTCTGACAA GAAGGGGCCA 
AAGACCATGG CAGAGATGCG GAAACAGGAA CTTGCCAGAG ATACAGACCC ACTCAAATTG 
AAGCTTTTGG ACTGGATTGA AGGCAAGGAG AGGAACATTC GTGCACTGCT GTCCACTCTG 
CACACGGTAT TGTGGGATGG GGAGAGCCGC TGGACACCTG TGAGTATGGC TGACCTGGTG 
ACTCCAGAGC AGGTGAAGAA GCAGTACCGC CGTGCAGTGC TGGTAGTGCA TCCTGATAAG 
GCCACAGGGC AGCCGTATGA ACAGTATGCC AAGATGATCT TCATGGAGCT GAATGATGCA 
TGGTCTGAGT TTGAAAACCA GGGCTCGAGG CCCCTCTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001268981.1
CDS	626..3907
Translation	MVEEEITRNTTPMYRTPEIVDLYSNFPIGEKQDIWALGCILYLLCFRQHPFEDGAKLRIVNGKYSIPVNDTRYTVFHDLIRAMLKVNPEERLSIAEVVRQLQEIAAARNVNPKAPITELLEQNGGYGNSGPSRAQPPCGGTVNSSGVLALAEYDQPYGGFLDILRGGTERLFTNLKDTSSKVIQSVANYAKGDLDISYITSRIAVMSFPAEGVESAIKNNIEDVRMFLDAKHPGHYAVYNLSPRIYRASKFHNRVTECGWAVRRAPHLHSLYTLCRSMHAWLREDHRNVCVVHCMDGRAASAVAVCAFLCFCRLFSTAEAAVYMFSMKRCPPGIWPSHKRYIEYVCDMVAEEPITPHSKPMLVKSVVMTPVPLFSKQRNGCRPFCEVYVGEERVTTTSQEYDRMKEFKIEDGKAVIPLGVTVQGDVLIIIYHARATLGGRLQAKMASMKMFQIQFHTGFVPRNATTVKFAKYDLDACDIQEKYPDLFQVNLEVEVEPRDRPSREAPPWENTSLRGLNPKILFSNREEQQDILSKFGKPELPRQPGSTAQYDAEAGSPEAEITESDSPQSSSTDTNHFLHTLDWQEEKEPETGLDNTSPKESQSVLIADGDGSEVSDEEEASFPSEERKPGAGEDTPRLAAGTKQQDLIFDVGMLAAPQEPVQPEEGVDLLGLHSEGDLRPAAPLQACGVPSSNTDLLSCLLEPSDAAQVGPPGDLLGGEAPLLLASPVSPLGLQNNLQGKVPDTVDPFDQFLLSSNSDTQPCSKPDLFGEFLNSDSVASSTAFPSTHSAPPPSCSTAFLHLGDLPAEPSKVIASSSHPDLLGGWDTWADTATPGPASIPVPEGTLFSSAGHPAPPGPNPSQTKSQNLDPFADLSDLSSSLQGLPAGLPAGGFVGAPAPTQKSNSPWQANRPTAPGTSWTPQAKPAPRASEQLRSHFSVIGAREERGVRVPSFAQKPKVSENDFEDLLPNQGFSKSDKKGPKTMAEMRKQELARDTDPLKLKLLDWIEGKERNIRALLSTLHTVLWDGESRWTPVSMADLVTPEQVKKQYRRAVLVVHPDKATGQPYEQYAKMIFMELNDAWSEFENQGSRPLF

Target ORF information:

RefSeq Version	NM_001282052.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus cyclin G associated kinase (Gak), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001282052.1

ORF Insert Sequence:

ATGGTGGAGG AAGAGATCAC AAGGAACACC ACACCCATGT ACAGAACACC AGAAATTGTA 
GACCTGTATT CCAACTTCCC CATTGGCGAA AAGCAGGATA TCTGGGCACT GGGCTGTATC 
TTATACCTGC TGTGTTTCCG GCAGCATCCT TTTGAGGATG GAGCAAAACT TCGGATAGTC 
AATGGGAAGT ATTCCATTCC TGTGAATGAC ACTCGTTACA CAGTCTTCCA TGACCTTATT 
CGTGCCATGC TAAAGGTCAA CCCAGAGGAG AGGCTGTCCA TTGCTGAAGT GGTCCGACAA 
CTGCAGGAAA TTGCAGCAGC TAGGAATGTG AACCCCAAAG CTCCCATCAC AGAGCTGCTG 
GAGCAGAATG GTGGCTATGG GAACTCAGGG CCTTCCCGAG CACAACCACC TTGCGGGGGC 
ACCGTGAACA GCAGTGGAGT TTTGGCTCTG GCAGAGTATG ACCAGCCCTA CGGTGGGTTT 
CTTGATATTC TGCGGGGTGG GACCGAACGG CTCTTCACCA ACCTCAAGGA TACTTCCTCC 
AAGGTCATCC AGTCTGTGGC TAACTATGCA AAGGGTGATC TGGACATATC TTACATCACA 
TCCAGGATTG CAGTGATGTC ATTCCCAGCA GAAGGTGTGG AGTCTGCAAT CAAAAACAAT 
ATAGAGGATG TGCGAATGTT TCTGGATGCT AAGCATCCAG GACATTATGC TGTCTACAAC 
CTTTCTCCAA GGATATACCG GGCTTCCAAG TTTCACAACC GGGTCACTGA GTGTGGCTGG 
GCAGTCAGAC GGGCACCACA TCTCCACAGT TTGTATACTC TATGTAGGAG CATGCATGCC 
TGGCTCCGGG AAGACCACAG GAACGTCTGT GTTGTGCATT GCATGGATGG GAGAGCTGCA 
TCTGCTGTGG CAGTCTGTGC ATTCCTGTGC TTCTGCCGTC TCTTCAGCAC TGCAGAGGCT 
GCTGTGTACA TGTTCAGCAT GAAGCGCTGC CCACCAGGCA TTTGGCCATC CCACAAAAGG 
TACATTGAAT ATGTATGTGA CATGGTGGCA GAGGAGCCCA TCACGCCCCA TAGCAAGCCA 
ATGCTGGTGA AATCTGTTGT CATGACCCCC GTGCCACTGT TCAGCAAGCA GAGGAATGGC 
TGCCGACCGT TCTGTGAGGT CTACGTTGGA GAGGAGCGTG TCACCACCAC GTCGCAGGAA 
TATGACAGAA TGAAGGAATT TAAAATTGAG GATGGCAAGG CTGTCATCCC CCTGGGCGTA 
ACAGTTCAAG GAGACGTGCT CATCATCATC TACCATGCCA GGGCCACACT GGGAGGGAGG 
CTGCAGGCTA AGATGGCGTC CATGAAAATG TTCCAGATCC AGTTCCACAC TGGGTTCGTG 
CCTCGAAACG CAACCACTGT GAAATTTGCA AAATATGACC TGGATGCTTG TGATATTCAA 
GAGAAGTACC CAGATCTGTT CCAGGTGAAC CTGGAAGTGG AGGTAGAGCC TAGAGACAGG 
CCCAGCCGAG AAGCTCCACC TTGGGAGAAC ACCAGCCTAA GGGGGTTAAA CCCCAAGATC 
CTCTTTTCCA ACAGGGAAGA GCAGCAGGAC ATTCTGTCTA AGTTTGGGAA GCCGGAGCTA 
CCCCGGCAGC CGGGCTCCAC AGCTCAGTAT GATGCTGAGG CAGGGTCTCC GGAGGCTGAG 
ATCACAGAGT CGGACTCACC GCAGAGCAGC AGTACGGACA CCAACCACTT TCTTCACACA 
CTGGATTGGC AGGAGGAAAA AGAACCAGAG ACTGGGTTAG ACAATACCTC TCCTAAGGAG 
AGTCAGTCTG TCCTGATTGC AGACGGAGAT GGAAGTGAAG TATCAGATGA AGAAGAGGCT 
TCATTCCCCA GTGAGGAGAG GAAACCAGGA GCTGGAGAAG ATACACCAAG GCTGGCTGCT 
GGGACCAAAC AGCAAGACTT AATATTTGAT GTGGGCATGC TGGCTGCCCC ACAGGAGCCT 
GTACAGCCCG AAGAAGGTGT TGATCTCCTG GGGCTACACT CTGAGGGGGA CTTAAGGCCT 
GCTGCCCCCT TACAGGCTTG TGGGGTCCCA TCTAGCAACA CTGACCTGTT GAGCTGCCTT 
CTTGAACCAT CTGATGCTGC TCAAGTAGGG CCTCCTGGTG ACCTGCTTGG TGGTGAGGCT 
CCACTGCTGC TAGCAAGCCC AGTTTCTCCT CTTGGGCTGC AGAACAACCT ACAAGGAAAA 
GTCCCTGACA CTGTGGACCC ATTTGACCAG TTCCTGCTGT CATCCAACTC AGACACCCAG 
CCCTGCTCCA AGCCTGATCT CTTTGGAGAG TTTCTCAACT CTGACTCTGT AGCTTCCTCA 
ACTGCCTTCC CATCCACCCA CAGTGCCCCA CCCCCATCCT GTAGCACTGC CTTCCTGCAC 
CTAGGGGATC TGCCAGCAGA GCCCAGCAAG GTAATAGCTT CATCCAGCCA CCCAGATCTG 
CTAGGAGGAT GGGATACGTG GGCTGACACA GCTACACCTG GGCCAGCCTC CATTCCAGTA 
CCAGAAGGTA CCCTCTTCTC TTCTGCAGGT CACCCAGCCC CTCCTGGCCC CAACCCCAGC 
CAAACCAAGT CTCAGAACCT TGACCCATTT GCTGATCTCA GTGATCTCAG CTCTAGCCTC 
CAAGGCCTGC CTGCTGGACT TCCTGCAGGG GGCTTTGTTG GCGCACCAGC CCCCACTCAA 
AAGAGCAACA GCCCCTGGCA GGCAAATCGT CCCACAGCCC CTGGAACCTC ATGGACCCCC 
CAGGCCAAGC CAGCCCCCAG AGCCTCTGAA CAGCTAAGGT CTCACTTCAG TGTGATCGGG 
GCCCGAGAAG AGAGGGGCGT CCGTGTGCCC AGCTTTGCCC AAAAGCCAAA GGTCTCAGAA 
AATGATTTTG AAGATCTTCT GCCTAATCAA GGCTTCTCTA AGTCTGACAA GAAGGGGCCA 
AAGACCATGG CAGAGATGCG GAAACAGGAA CTTGCCAGAG ATACAGACCC ACTCAAATTG 
AAGCTTTTGG ACTGGATTGA AGGCAAGGAG AGGAACATTC GTGCACTGCT GTCCACTCTG 
CACACGGTAT TGTGGGATGG GGAGAGCCGC TGGACACCTG TGAGTATGGC TGACCTGGTG 
ACTCCAGAGC AGGTGAAGAA GCAGTACCGC CGTGCAGTGC TGGTAGTGCA TCCTGATAAG 
GCCACAGGGC AGCCGTATGA ACAGTATGCC AAGATGATCT TCATGGAGCT GAATGATGCA 
TGGTCTGAGT TTGAAAACCA GGGCTCGAGG CCCCTCTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gak ( NM_153569.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_705797.1 Mus musculus cyclin G associated kinase (Gak), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu21914
Clone ID Related Accession (Same CDS sequence)	NM_153569.2
Accession Version	NM_153569.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3918bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	cyclin-G-associated kinase isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY312464.1, AK166682.1, AI466057.1 and AC133896.7. On Aug 15, 2013 this sequence version replaced NM_153569.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK146695.1, AK166682.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGCTGC TGCAGTCTGC GCTGGACTTC CTGGCGGGCC CTGGTTCTCT TGGCGGAGCT 
GCGGGCCGTG ATCAGAGTGA CTTCGTGGGG CAGACAGTGG AGCTGGGCGA GCTGCGTCTG 
CGGGTGCGGC GGGTCCTGGC CGAGGGAGGG TTTGCTTTTG TTTATGAAGC TCAAGATCTG 
GGAAGTGGCA GAGAGTATGC ATTAAAGCGA TTACTATCCA ATGAAGAGGA AAAGAACAGA 
GCCATCATTC AGGAAGTTTG TTTCTTGAAA AAACTTTCTG GCCACCCCAA TATTGTCCAG 
TTCTGCTCTG CAGCATCCAT AGGAAAAGAG GAGTCGGACA CTGGGCAGGC TGAGTTCCTC 
CTGCTTACGG AGCTTTGTAA AGGACAGCTG GTGGAGTTTC TCAAGAGAGT TGAATGTAAA 
GGTCCTCTGT CCTGCGACAG CATTCTGAAG ATCTTCTACC AGACATGCAG AGCAGTGCAG 
CACATGCACA GGCAGAAACC ACCCATCATC CACAGAGATC TCAAGGTTGA AAACTTACTG 
CTTAGTAACC AGGGGACCAT TAAGCTGTGT GACTTTGGCA GTGCCACAAC CATCTCCCAT 
TATCCTGACT ACAGCTGGAG TGCCCAGAAG CGAGCCATGG TGGAGGAAGA GATCACAAGG 
AACACCACAC CCATGTACAG AACACCAGAA ATTGTAGACC TGTATTCCAA CTTCCCCATT 
GGCGAAAAGC AGGATATCTG GGCACTGGGC TGTATCTTAT ACCTGCTGTG TTTCCGGCAG 
CATCCTTTTG AGGATGGAGC AAAACTTCGG ATAGTCAATG GGAAGTATTC CATTCCTGTG 
AATGACACTC GTTACACAGT CTTCCATGAC CTTATTCGTG CCATGCTAAA GGTCAACCCA 
GAGGAGAGGC TGTCCATTGC TGAAGTGGTC CGACAACTGC AGGAAATTGC AGCAGCTAGG 
AATGTGAACC CCAAAGCTCC CATCACAGAG CTGCTGGAGC AGAATGGTGG CTATGGGAAC 
TCAGGGCCTT CCCGAGCACA ACCACCTTGC GGGGGCACCG TGAACAGCAG TGGAGTTTTG 
GCTCTGGCAG AGTATGACCA GCCCTACGGT GGGTTTCTTG ATATTCTGCG GGGTGGGACC 
GAACGGCTCT TCACCAACCT CAAGGATACT TCCTCCAAGG TCATCCAGTC TGTGGCTAAC 
TATGCAAAGG GTGATCTGGA CATATCTTAC ATCACATCCA GGATTGCAGT GATGTCATTC 
CCAGCAGAAG GTGTGGAGTC TGCAATCAAA AACAATATAG AGGATGTGCG AATGTTTCTG 
GATGCTAAGC ATCCAGGACA TTATGCTGTC TACAACCTTT CTCCAAGGAT ATACCGGGCT 
TCCAAGTTTC ACAACCGGGT CACTGAGTGT GGCTGGGCAG TCAGACGGGC ACCACATCTC 
CACAGTTTGT ATACTCTATG TAGGAGCATG CATGCCTGGC TCCGGGAAGA CCACAGGAAC 
GTCTGTGTTG TGCATTGCAT GGATGGGAGA GCTGCATCTG CTGTGGCAGT CTGTGCATTC 
CTGTGCTTCT GCCGTCTCTT CAGCACTGCA GAGGCTGCTG TGTACATGTT CAGCATGAAG 
CGCTGCCCAC CAGGCATTTG GCCATCCCAC AAAAGGTACA TTGAATATGT ATGTGACATG 
GTGGCAGAGG AGCCCATCAC GCCCCATAGC AAGCCAATGC TGGTGAAATC TGTTGTCATG 
ACCCCCGTGC CACTGTTCAG CAAGCAGAGG AATGGCTGCC GACCGTTCTG TGAGGTCTAC 
GTTGGAGAGG AGCGTGTCAC CACCACGTCG CAGGAATATG ACAGAATGAA GGAATTTAAA 
ATTGAGGATG GCAAGGCTGT CATCCCCCTG GGCGTAACAG TTCAAGGAGA CGTGCTCATC 
ATCATCTACC ATGCCAGGGC CACACTGGGA GGGAGGCTGC AGGCTAAGAT GGCGTCCATG 
AAAATGTTCC AGATCCAGTT CCACACTGGG TTCGTGCCTC GAAACGCAAC CACTGTGAAA 
TTTGCAAAAT ATGACCTGGA TGCTTGTGAT ATTCAAGAGA AGTACCCAGA TCTGTTCCAG 
GTGAACCTGG AAGTGGAGGT AGAGCCTAGA GACAGGCCCA GCCGAGAAGC TCCACCTTGG 
GAGAACACCA GCCTAAGGGG GTTAAACCCC AAGATCCTCT TTTCCAACAG GGAAGAGCAG 
CAGGACATTC TGTCTAAGTT TGGGAAGCCG GAGCTACCCC GGCAGCCGGG CTCCACAGCT 
CAGTATGATG CTGAGGCAGG GTCTCCGGAG GCTGAGATCA CAGAGTCGGA CTCACCGCAG 
AGCAGCAGTA CGGACACCAA CCACTTTCTT CACACACTGG ATTGGCAGGA GGAAAAAGAA 
CCAGAGACTG GGTTAGACAA TACCTCTCCT AAGGAGAGTC AGTCTGTCCT GATTGCAGAC 
GGAGATGGAA GTGAAGTATC AGATGAAGAA GAGGCTTCAT TCCCCAGTGA GGAGAGGAAA 
CCAGGAGCTG GAGAAGATAC ACCAAGGCTG GCTGCTGGGA CCAAACAGCA AGACTTAATA 
TTTGATGTGG GCATGCTGGC TGCCCCACAG GAGCCTGTAC AGCCCGAAGA AGGTGTTGAT 
CTCCTGGGGC TACACTCTGA GGGGGACTTA AGGCCTGCTG CCCCCTTACA GGCTTGTGGG 
GTCCCATCTA GCAACACTGA CCTGTTGAGC TGCCTTCTTG AACCATCTGA TGCTGCTCAA 
GTAGGGCCTC CTGGTGACCT GCTTGGTGGT GAGGCTCCAC TGCTGCTAGC AAGCCCAGTT 
TCTCCTCTTG GGCTGCAGAA CAACCTACAA GGAAAAGTCC CTGACACTGT GGACCCATTT 
GACCAGTTCC TGCTGTCATC CAACTCAGAC ACCCAGCCCT GCTCCAAGCC TGATCTCTTT 
GGAGAGTTTC TCAACTCTGA CTCTGTAGCT TCCTCAACTG CCTTCCCATC CACCCACAGT 
GCCCCACCCC CATCCTGTAG CACTGCCTTC CTGCACCTAG GGGATCTGCC AGCAGAGCCC 
AGCAAGGTAA TAGCTTCATC CAGCCACCCA GATCTGCTAG GAGGATGGGA TACGTGGGCT 
GACACAGCTA CACCTGGGCC AGCCTCCATT CCAGTACCAG AAGGTACCCT CTTCTCTTCT 
GCAGGTCACC CAGCCCCTCC TGGCCCCAAC CCCAGCCAAA CCAAGTCTCA GAACCTTGAC 
CCATTTGCTG ATCTCAGTGA TCTCAGCTCT AGCCTCCAAG GCCTGCCTGC TGGACTTCCT 
GCAGGGGGCT TTGTTGGCGC ACCAGCCCCC ACTCAAAAGA GCAACAGCCC CTGGCAGGCA 
AATCGTCCCA CAGCCCCTGG AACCTCATGG ACCCCCCAGG CCAAGCCAGC CCCCAGAGCC 
TCTGAACAGC TAAGGTCTCA CTTCAGTGTG ATCGGGGCCC GAGAAGAGAG GGGCGTCCGT 
GTGCCCAGCT TTGCCCAAAA GCCAAAGGTC TCAGAAAATG ATTTTGAAGA TCTTCTGCCT 
AATCAAGGCT TCTCTAAGTC TGACAAGAAG GGGCCAAAGA CCATGGCAGA GATGCGGAAA 
CAGGAACTTG CCAGAGATAC AGACCCACTC AAATTGAAGC TTTTGGACTG GATTGAAGGC 
AAGGAGAGGA ACATTCGTGC ACTGCTGTCC ACTCTGCACA CGGTATTGTG GGATGGGGAG 
AGCCGCTGGA CACCTGTGAG TATGGCTGAC CTGGTGACTC CAGAGCAGGT GAAGAAGCAG 
TACCGCCGTG CAGTGCTGGT AGTGCATCCT GATAAGGCCA CAGGGCAGCC GTATGAACAG 
TATGCCAAGA TGATCTTCAT GGAGCTGAAT GATGCATGGT CTGAGTTTGA AAACCAGGGC 
TCGAGGCCCC TCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_705797.1
CDS	133..4050
Translation	MSLLQSALDFLAGPGSLGGAAGRDQSDFVGQTVELGELRLRVRRVLAEGGFAFVYEAQDLGSGREYALKRLLSNEEEKNRAIIQEVCFLKKLSGHPNIVQFCSAASIGKEESDTGQAEFLLLTELCKGQLVEFLKRVECKGPLSCDSILKIFYQTCRAVQHMHRQKPPIIHRDLKVENLLLSNQGTIKLCDFGSATTISHYPDYSWSAQKRAMVEEEITRNTTPMYRTPEIVDLYSNFPIGEKQDIWALGCILYLLCFRQHPFEDGAKLRIVNGKYSIPVNDTRYTVFHDLIRAMLKVNPEERLSIAEVVRQLQEIAAARNVNPKAPITELLEQNGGYGNSGPSRAQPPCGGTVNSSGVLALAEYDQPYGGFLDILRGGTERLFTNLKDTSSKVIQSVANYAKGDLDISYITSRIAVMSFPAEGVESAIKNNIEDVRMFLDAKHPGHYAVYNLSPRIYRASKFHNRVTECGWAVRRAPHLHSLYTLCRSMHAWLREDHRNVCVVHCMDGRAASAVAVCAFLCFCRLFSTAEAAVYMFSMKRCPPGIWPSHKRYIEYVCDMVAEEPITPHSKPMLVKSVVMTPVPLFSKQRNGCRPFCEVYVGEERVTTTSQEYDRMKEFKIEDGKAVIPLGVTVQGDVLIIIYHARATLGGRLQAKMASMKMFQIQFHTGFVPRNATTVKFAKYDLDACDIQEKYPDLFQVNLEVEVEPRDRPSREAPPWENTSLRGLNPKILFSNREEQQDILSKFGKPELPRQPGSTAQYDAEAGSPEAEITESDSPQSSSTDTNHFLHTLDWQEEKEPETGLDNTSPKESQSVLIADGDGSEVSDEEEASFPSEERKPGAGEDTPRLAAGTKQQDLIFDVGMLAAPQEPVQPEEGVDLLGLHSEGDLRPAAPLQACGVPSSNTDLLSCLLEPSDAAQVGPPGDLLGGEAPLLLASPVSPLGLQNNLQGKVPDTVDPFDQFLLSSNSDTQPCSKPDLFGEFLNSDSVASSTAFPSTHSAPPPSCSTAFLHLGDLPAEPSKVIASSSHPDLLGGWDTWADTATPGPASIPVPEGTLFSSAGHPAPPGPNPSQTKSQNLDPFADLSDLSSSLQGLPAGLPAGGFVGAPAPTQKSNSPWQANRPTAPGTSWTPQAKPAPRASEQLRSHFSVIGAREERGVRVPSFAQKPKVSENDFEDLLPNQGFSKSDKKGPKTMAEMRKQELARDTDPLKLKLLDWIEGKERNIRALLSTLHTVLWDGESRWTPVSMADLVTPEQVKKQYRRAVLVVHPDKATGQPYEQYAKMIFMELNDAWSEFENQGSRPLF

Target ORF information:

RefSeq Version	NM_153569.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus cyclin G associated kinase (Gak), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_153569.2

ORF Insert Sequence:

ATGTCGCTGC TGCAGTCTGC GCTGGACTTC CTGGCGGGCC CTGGTTCTCT TGGCGGAGCT 
GCGGGCCGTG ATCAGAGTGA CTTCGTGGGG CAGACAGTGG AGCTGGGCGA GCTGCGTCTG 
CGGGTGCGGC GGGTCCTGGC CGAGGGAGGG TTTGCTTTTG TTTATGAAGC TCAAGATCTG 
GGAAGTGGCA GAGAGTATGC ATTAAAGCGA TTACTATCCA ATGAAGAGGA AAAGAACAGA 
GCCATCATTC AGGAAGTTTG TTTCTTGAAA AAACTTTCTG GCCACCCCAA TATTGTCCAG 
TTCTGCTCTG CAGCATCCAT AGGAAAAGAG GAGTCGGACA CTGGGCAGGC TGAGTTCCTC 
CTGCTTACGG AGCTTTGTAA AGGACAGCTG GTGGAGTTTC TCAAGAGAGT TGAATGTAAA 
GGTCCTCTGT CCTGCGACAG CATTCTGAAG ATCTTCTACC AGACATGCAG AGCAGTGCAG 
CACATGCACA GGCAGAAACC ACCCATCATC CACAGAGATC TCAAGGTTGA AAACTTACTG 
CTTAGTAACC AGGGGACCAT TAAGCTGTGT GACTTTGGCA GTGCCACAAC CATCTCCCAT 
TATCCTGACT ACAGCTGGAG TGCCCAGAAG CGAGCCATGG TGGAGGAAGA GATCACAAGG 
AACACCACAC CCATGTACAG AACACCAGAA ATTGTAGACC TGTATTCCAA CTTCCCCATT 
GGCGAAAAGC AGGATATCTG GGCACTGGGC TGTATCTTAT ACCTGCTGTG TTTCCGGCAG 
CATCCTTTTG AGGATGGAGC AAAACTTCGG ATAGTCAATG GGAAGTATTC CATTCCTGTG 
AATGACACTC GTTACACAGT CTTCCATGAC CTTATTCGTG CCATGCTAAA GGTCAACCCA 
GAGGAGAGGC TGTCCATTGC TGAAGTGGTC CGACAACTGC AGGAAATTGC AGCAGCTAGG 
AATGTGAACC CCAAAGCTCC CATCACAGAG CTGCTGGAGC AGAATGGTGG CTATGGGAAC 
TCAGGGCCTT CCCGAGCACA ACCACCTTGC GGGGGCACCG TGAACAGCAG TGGAGTTTTG 
GCTCTGGCAG AGTATGACCA GCCCTACGGT GGGTTTCTTG ATATTCTGCG GGGTGGGACC 
GAACGGCTCT TCACCAACCT CAAGGATACT TCCTCCAAGG TCATCCAGTC TGTGGCTAAC 
TATGCAAAGG GTGATCTGGA CATATCTTAC ATCACATCCA GGATTGCAGT GATGTCATTC 
CCAGCAGAAG GTGTGGAGTC TGCAATCAAA AACAATATAG AGGATGTGCG AATGTTTCTG 
GATGCTAAGC ATCCAGGACA TTATGCTGTC TACAACCTTT CTCCAAGGAT ATACCGGGCT 
TCCAAGTTTC ACAACCGGGT CACTGAGTGT GGCTGGGCAG TCAGACGGGC ACCACATCTC 
CACAGTTTGT ATACTCTATG TAGGAGCATG CATGCCTGGC TCCGGGAAGA CCACAGGAAC 
GTCTGTGTTG TGCATTGCAT GGATGGGAGA GCTGCATCTG CTGTGGCAGT CTGTGCATTC 
CTGTGCTTCT GCCGTCTCTT CAGCACTGCA GAGGCTGCTG TGTACATGTT CAGCATGAAG 
CGCTGCCCAC CAGGCATTTG GCCATCCCAC AAAAGGTACA TTGAATATGT ATGTGACATG 
GTGGCAGAGG AGCCCATCAC GCCCCATAGC AAGCCAATGC TGGTGAAATC TGTTGTCATG 
ACCCCCGTGC CACTGTTCAG CAAGCAGAGG AATGGCTGCC GACCGTTCTG TGAGGTCTAC 
GTTGGAGAGG AGCGTGTCAC CACCACGTCG CAGGAATATG ACAGAATGAA GGAATTTAAA 
ATTGAGGATG GCAAGGCTGT CATCCCCCTG GGCGTAACAG TTCAAGGAGA CGTGCTCATC 
ATCATCTACC ATGCCAGGGC CACACTGGGA GGGAGGCTGC AGGCTAAGAT GGCGTCCATG 
AAAATGTTCC AGATCCAGTT CCACACTGGG TTCGTGCCTC GAAACGCAAC CACTGTGAAA 
TTTGCAAAAT ATGACCTGGA TGCTTGTGAT ATTCAAGAGA AGTACCCAGA TCTGTTCCAG 
GTGAACCTGG AAGTGGAGGT AGAGCCTAGA GACAGGCCCA GCCGAGAAGC TCCACCTTGG 
GAGAACACCA GCCTAAGGGG GTTAAACCCC AAGATCCTCT TTTCCAACAG GGAAGAGCAG 
CAGGACATTC TGTCTAAGTT TGGGAAGCCG GAGCTACCCC GGCAGCCGGG CTCCACAGCT 
CAGTATGATG CTGAGGCAGG GTCTCCGGAG GCTGAGATCA CAGAGTCGGA CTCACCGCAG 
AGCAGCAGTA CGGACACCAA CCACTTTCTT CACACACTGG ATTGGCAGGA GGAAAAAGAA 
CCAGAGACTG GGTTAGACAA TACCTCTCCT AAGGAGAGTC AGTCTGTCCT GATTGCAGAC 
GGAGATGGAA GTGAAGTATC AGATGAAGAA GAGGCTTCAT TCCCCAGTGA GGAGAGGAAA 
CCAGGAGCTG GAGAAGATAC ACCAAGGCTG GCTGCTGGGA CCAAACAGCA AGACTTAATA 
TTTGATGTGG GCATGCTGGC TGCCCCACAG GAGCCTGTAC AGCCCGAAGA AGGTGTTGAT 
CTCCTGGGGC TACACTCTGA GGGGGACTTA AGGCCTGCTG CCCCCTTACA GGCTTGTGGG 
GTCCCATCTA GCAACACTGA CCTGTTGAGC TGCCTTCTTG AACCATCTGA TGCTGCTCAA 
GTAGGGCCTC CTGGTGACCT GCTTGGTGGT GAGGCTCCAC TGCTGCTAGC AAGCCCAGTT 
TCTCCTCTTG GGCTGCAGAA CAACCTACAA GGAAAAGTCC CTGACACTGT GGACCCATTT 
GACCAGTTCC TGCTGTCATC CAACTCAGAC ACCCAGCCCT GCTCCAAGCC TGATCTCTTT 
GGAGAGTTTC TCAACTCTGA CTCTGTAGCT TCCTCAACTG CCTTCCCATC CACCCACAGT 
GCCCCACCCC CATCCTGTAG CACTGCCTTC CTGCACCTAG GGGATCTGCC AGCAGAGCCC 
AGCAAGGTAA TAGCTTCATC CAGCCACCCA GATCTGCTAG GAGGATGGGA TACGTGGGCT 
GACACAGCTA CACCTGGGCC AGCCTCCATT CCAGTACCAG AAGGTACCCT CTTCTCTTCT 
GCAGGTCACC CAGCCCCTCC TGGCCCCAAC CCCAGCCAAA CCAAGTCTCA GAACCTTGAC 
CCATTTGCTG ATCTCAGTGA TCTCAGCTCT AGCCTCCAAG GCCTGCCTGC TGGACTTCCT 
GCAGGGGGCT TTGTTGGCGC ACCAGCCCCC ACTCAAAAGA GCAACAGCCC CTGGCAGGCA 
AATCGTCCCA CAGCCCCTGG AACCTCATGG ACCCCCCAGG CCAAGCCAGC CCCCAGAGCC 
TCTGAACAGC TAAGGTCTCA CTTCAGTGTG ATCGGGGCCC GAGAAGAGAG GGGCGTCCGT 
GTGCCCAGCT TTGCCCAAAA GCCAAAGGTC TCAGAAAATG ATTTTGAAGA TCTTCTGCCT 
AATCAAGGCT TCTCTAAGTC TGACAAGAAG GGGCCAAAGA CCATGGCAGA GATGCGGAAA 
CAGGAACTTG CCAGAGATAC AGACCCACTC AAATTGAAGC TTTTGGACTG GATTGAAGGC 
AAGGAGAGGA ACATTCGTGC ACTGCTGTCC ACTCTGCACA CGGTATTGTG GGATGGGGAG 
AGCCGCTGGA CACCTGTGAG TATGGCTGAC CTGGTGACTC CAGAGCAGGT GAAGAAGCAG 
TACCGCCGTG CAGTGCTGGT AGTGCATCCT GATAAGGCCA CAGGGCAGCC GTATGAACAG 
TATGCCAAGA TGATCTTCAT GGAGCTGAAT GATGCATGGT CTGAGTTTGA AAACCAGGGC 
TCGAGGCCCC TCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gak ( NM_001359922.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001346851.1 Mus musculus cyclin G associated kinase (Gak), transcript variant 4, mRNA.

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CloneID	OMu81362
Clone ID Related Accession (Same CDS sequence)	XM_017320853.1 , NM_001359922.1
Accession Version	NM_001359922.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3288bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-12
Organism	Mus musculus(house mouse)
Product	cyclin-G-associated kinase isoform 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC161813.2 and AC133896.7. On Jan 19, 2018 this sequence version replaced XM_017320853.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660815.217894.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCACAGGC AGAAACCACC CATCATCCAC AGAGATCTCA AGGTTGAAAA CTTACTGCTT 
AGTAACCAGG GGACCATTAA GCTGTGTGAC TTTGGCAGTG CCACAACCAT CTCCCATTAT 
CCTGACTACA GCTGGAGTGC CCAGAAGCGA GCCATGGTGG AGGAAGAGAT CACAAGGAAC 
ACCACACCCA TGTACAGAAC ACCAGAAATT GTAGACCTGT ATTCCAACTT CCCCATTGGC 
GAAAAGCAGG ATATCTGGGC ACTGGGCTGT ATCTTATACC TGCTGTGTTT CCGGCAGCAT 
CCTTTTGAGG ATGGAGCAAA ACTTCGGATA GTCAATGGGA AGTATTCCAT TCCTGTGAAT 
GACACTCGTT ACACAGTCTT CCATGACCTT ATTCGTGCCA TGCTAAAGGT CAACCCAGAG 
GAGAGGCTGT CCATTGCTGA AGTGGTCCGA CAACTGCAGG AAATTGCAGC AGCTAGGAAT 
GTGAACCCCA AAGCTCCCAT CACAGAGCTG CTGGAGCAGA ATGGTGGCTA TGGGAACTCA 
GGGCCTTCCC GAGCACAACC ACCTTGCGGG GGCACCGTGA ACAGCAGTGG AGTTTTGGCT 
CTGGCAGAGT ATGACCAGCC CTACGGTGGG TTTCTTGATA TTCTGCGGGG TGGGACCGAA 
CGGCTCTTCA CCAACCTCAA GGATACTTCC TCCAAGGTCA TCCAGTCTGT GGCTAACTAT 
GCAAAGGGTG ATCTGGACAT ATCTTACATC ACATCCAGGA TTGCAGTGAT GTCATTCCCA 
GCAGAAGGTG TGGAGTCTGC AATCAAAAAC AATATAGAGG ATGTGCGAAT GTTTCTGGAT 
GCTAAGCATC CAGGACATTA TGCTGTCTAC AACCTTTCTC CAAGGATATA CCGGGCTTCC 
AAGTTTCACA ACCGGGTCAC TGAGTGTGGC TGGGCAGTCA GACGGGCACC ACATCTCCAC 
AGTTTGTATA CTCTATGTAG GAGCATGCAT GCCTGGCTCC GGGAAGACCA CAGGAACGTC 
TGTGTTGTGC ATTGCATGGA TGGGAGAGCT GCATCTGCTG TGGCAGTCTG TGCATTCCTG 
TGCTTCTGCC GTCTCTTCAG CACTGCAGAG GCTGCTGTGT ACATGTTCAG CATGAAGCGC 
TGCCCACCAG GCATTTGGCC ATCCCACAAA AGGTACATTG AATATGTATG TGACATGGTG 
GCAGAGGAGC CCATCACGCC CCATAGCAAG CCAATGCTGG TGAAATCTGT TGTCATGACC 
CCCGTGCCAC TGTTCAGCAA GCAGAGGAAT GGCTGCCGAC CGTTCTGTGA GGTCTACGTT 
GGAGAGGAGC GTGTCACCAC CACGTCGCAG GAATATGACA GAATGAAGGA ATTTAAAATT 
GAGGATGGCA AGGCTGTCAT CCCCCTGGGC GTAACAGTTC AAGGAGACGT GCTCATCATC 
ATCTACCATG CCAGGGCCAC ACTGGGAGGG AGGCTGCAGG CTAAGATGGC GTCCATGAAA 
ATGTTCCAGA TCCAGTTCCA CACTGGGTTC GTGCCTCGAA ACGCAACCAC TGTGAAATTT 
GCAAAATATG ACCTGGATGC TTGTGATATT CAAGAGAAGT ACCCAGATCT GTTCCAGGTG 
AACCTGGAAG TGGAGGTAGA GCCTAGAGAC AGGCCCAGCC GAGAAGCTCC ACCTTGGGAG 
AACACCAGCC TAAGGGGGTT AAACCCCAAG ATCCTCTTTT CCAACAGGGA AGAGCAGCAG 
GACATTCTGT CTAAGTTTGA GGAAAAAGAA CCAGAGACTG GGTTAGACAA TACCTCTCCT 
AAGGAGAGTC AGTCTGTCCT GATTGCAGAC GGAGATGGAA GTGAAGTATC AGATGAAGAA 
GAGGCTTCAT TCCCCAGTGA GGAGAGGAAA CCAGGAGCTG GAGAAGATAC ACCAAGGCTG 
GCTGCTGGGA CCAAACAGCA AGACTTAATA TTTGATGTGG GCATGCTGGC TGCCCCACAG 
GAGCCTGTAC AGCCCGAAGA AGGTGTTGAT CTCCTGGGGC TACACTCTGA GGGGGACTTA 
AGGCCTGCTG CCCCCTTACA GGCTTGTGGG GTCCCATCTA GCAACACTGA CCTGTTGAGC 
TGCCTTCTTG AACCATCTGA TGCTGCTCAA GTAGGGCCTC CTGGTGACCT GCTTGGTGGT 
GAGGCTCCAC TGCTGCTAGC AAGCCCAGTT TCTCCTCTTG GGCTGCAGAA CAACCTACAA 
GGAAAAGTCC CTGACACTGT GGACCCATTT GACCAGTTCC TGCTGTCATC CAACTCAGAC 
ACCCAGCCCT GCTCCAAGCC TGATCTCTTT GGAGAGTTTC TCAACTCTGA CTCTGTAGCT 
TCCTCAACTG CCTTCCCATC CACCCACAGT GCCCCACCCC CATCCTGTAG CACTGCCTTC 
CTGCACCTAG GGGATCTGCC AGCAGAGCCC AGCAAGGTAA TAGCTTCATC CAGCCACCCA 
GATCTGCTAG GAGGATGGGA TACGTGGGCT GACACAGCTA CACCTGGGCC AGCCTCCATT 
CCAGTACCAG AAGGTACCCT CTTCTCTTCT GCAGGTCACC CAGCCCCTCC TGGCCCCAAC 
CCCAGCCAAA CCAAGTCTCA GAACCTTGAC CCATTTGCTG ATCTCAGTGA TCTCAGCTCT 
AGCCTCCAAG GCCTGCCTGC TGGACTTCCT GCAGGGGGCT TTGTTGGCGC ACCAGCCCCC 
ACTCAAAAGA GCAACAGCCC CTGGCAGGCA AATCGTCCCA CAGCCCCTGG AACCTCATGG 
ACCCCCCAGG CCAAGCCAGC CCCCAGAGCC TCTGAACAGC TAAGGTCTCA CTTCAGTGTG 
ATCGGGGCCC GAGAAGAGAG GGGCGTCCGT GTGCCCAGCT TTGCCCAAAA GCCAAAGGTC 
TCAGAAAATG ATTTTGAAGA TCTTCTGCCT AATCAAGGCT TCTCTAAGTC TGACAAGAAG 
GGGCCAAAGA CCATGGCAGA GATGCGGAAA CAGGAACTTG CCAGAGATAC AGACCCACTC 
AAATTGAAGC TTTTGGACTG GATTGAAGGC AAGGAGAGGA ACATTCGTGC ACTGCTGTCC 
ACTCTGCACA CGGTATTGTG GGATGGGGAG AGCCGCTGGA CACCTGTGAG TATGGCTGAC 
CTGGTGACTC CAGAGCAGGT GAAGAAGCAG TACCGCCGTG CAGTGCTGGT AGTGCATCCT 
GATAAGGCCA CAGGGCAGCC GTATGAACAG TATGCCAAGA TGATCTTCAT GGAGCTGAAT 
GATGCATGGT CTGAGTTTGA AAACCAGGGC TCGAGGCCCC TCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001346851.1
CDS	611..3898
Translation	MHRQKPPIIHRDLKVENLLLSNQGTIKLCDFGSATTISHYPDYSWSAQKRAMVEEEITRNTTPMYRTPEIVDLYSNFPIGEKQDIWALGCILYLLCFRQHPFEDGAKLRIVNGKYSIPVNDTRYTVFHDLIRAMLKVNPEERLSIAEVVRQLQEIAAARNVNPKAPITELLEQNGGYGNSGPSRAQPPCGGTVNSSGVLALAEYDQPYGGFLDILRGGTERLFTNLKDTSSKVIQSVANYAKGDLDISYITSRIAVMSFPAEGVESAIKNNIEDVRMFLDAKHPGHYAVYNLSPRIYRASKFHNRVTECGWAVRRAPHLHSLYTLCRSMHAWLREDHRNVCVVHCMDGRAASAVAVCAFLCFCRLFSTAEAAVYMFSMKRCPPGIWPSHKRYIEYVCDMVAEEPITPHSKPMLVKSVVMTPVPLFSKQRNGCRPFCEVYVGEERVTTTSQEYDRMKEFKIEDGKAVIPLGVTVQGDVLIIIYHARATLGGRLQAKMASMKMFQIQFHTGFVPRNATTVKFAKYDLDACDIQEKYPDLFQVNLEVEVEPRDRPSREAPPWENTSLRGLNPKILFSNREEQQDILSKFEEKEPETGLDNTSPKESQSVLIADGDGSEVSDEEEASFPSEERKPGAGEDTPRLAAGTKQQDLIFDVGMLAAPQEPVQPEEGVDLLGLHSEGDLRPAAPLQACGVPSSNTDLLSCLLEPSDAAQVGPPGDLLGGEAPLLLASPVSPLGLQNNLQGKVPDTVDPFDQFLLSSNSDTQPCSKPDLFGEFLNSDSVASSTAFPSTHSAPPPSCSTAFLHLGDLPAEPSKVIASSSHPDLLGGWDTWADTATPGPASIPVPEGTLFSSAGHPAPPGPNPSQTKSQNLDPFADLSDLSSSLQGLPAGLPAGGFVGAPAPTQKSNSPWQANRPTAPGTSWTPQAKPAPRASEQLRSHFSVIGAREERGVRVPSFAQKPKVSENDFEDLLPNQGFSKSDKKGPKTMAEMRKQELARDTDPLKLKLLDWIEGKERNIRALLSTLHTVLWDGESRWTPVSMADLVTPEQVKKQYRRAVLVVHPDKATGQPYEQYAKMIFMELNDAWSEFENQGSRPLF

Target ORF information:

RefSeq Version	NM_001359922.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus cyclin G associated kinase (Gak), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001359922.1

ORF Insert Sequence:

ATGCACAGGC AGAAACCACC CATCATCCAC AGAGATCTCA AGGTTGAAAA CTTACTGCTT 
AGTAACCAGG GGACCATTAA GCTGTGTGAC TTTGGCAGTG CCACAACCAT CTCCCATTAT 
CCTGACTACA GCTGGAGTGC CCAGAAGCGA GCCATGGTGG AGGAAGAGAT CACAAGGAAC 
ACCACACCCA TGTACAGAAC ACCAGAAATT GTAGACCTGT ATTCCAACTT CCCCATTGGC 
GAAAAGCAGG ATATCTGGGC ACTGGGCTGT ATCTTATACC TGCTGTGTTT CCGGCAGCAT 
CCTTTTGAGG ATGGAGCAAA ACTTCGGATA GTCAATGGGA AGTATTCCAT TCCTGTGAAT 
GACACTCGTT ACACAGTCTT CCATGACCTT ATTCGTGCCA TGCTAAAGGT CAACCCAGAG 
GAGAGGCTGT CCATTGCTGA AGTGGTCCGA CAACTGCAGG AAATTGCAGC AGCTAGGAAT 
GTGAACCCCA AAGCTCCCAT CACAGAGCTG CTGGAGCAGA ATGGTGGCTA TGGGAACTCA 
GGGCCTTCCC GAGCACAACC ACCTTGCGGG GGCACCGTGA ACAGCAGTGG AGTTTTGGCT 
CTGGCAGAGT ATGACCAGCC CTACGGTGGG TTTCTTGATA TTCTGCGGGG TGGGACCGAA 
CGGCTCTTCA CCAACCTCAA GGATACTTCC TCCAAGGTCA TCCAGTCTGT GGCTAACTAT 
GCAAAGGGTG ATCTGGACAT ATCTTACATC ACATCCAGGA TTGCAGTGAT GTCATTCCCA 
GCAGAAGGTG TGGAGTCTGC AATCAAAAAC AATATAGAGG ATGTGCGAAT GTTTCTGGAT 
GCTAAGCATC CAGGACATTA TGCTGTCTAC AACCTTTCTC CAAGGATATA CCGGGCTTCC 
AAGTTTCACA ACCGGGTCAC TGAGTGTGGC TGGGCAGTCA GACGGGCACC ACATCTCCAC 
AGTTTGTATA CTCTATGTAG GAGCATGCAT GCCTGGCTCC GGGAAGACCA CAGGAACGTC 
TGTGTTGTGC ATTGCATGGA TGGGAGAGCT GCATCTGCTG TGGCAGTCTG TGCATTCCTG 
TGCTTCTGCC GTCTCTTCAG CACTGCAGAG GCTGCTGTGT ACATGTTCAG CATGAAGCGC 
TGCCCACCAG GCATTTGGCC ATCCCACAAA AGGTACATTG AATATGTATG TGACATGGTG 
GCAGAGGAGC CCATCACGCC CCATAGCAAG CCAATGCTGG TGAAATCTGT TGTCATGACC 
CCCGTGCCAC TGTTCAGCAA GCAGAGGAAT GGCTGCCGAC CGTTCTGTGA GGTCTACGTT 
GGAGAGGAGC GTGTCACCAC CACGTCGCAG GAATATGACA GAATGAAGGA ATTTAAAATT 
GAGGATGGCA AGGCTGTCAT CCCCCTGGGC GTAACAGTTC AAGGAGACGT GCTCATCATC 
ATCTACCATG CCAGGGCCAC ACTGGGAGGG AGGCTGCAGG CTAAGATGGC GTCCATGAAA 
ATGTTCCAGA TCCAGTTCCA CACTGGGTTC GTGCCTCGAA ACGCAACCAC TGTGAAATTT 
GCAAAATATG ACCTGGATGC TTGTGATATT CAAGAGAAGT ACCCAGATCT GTTCCAGGTG 
AACCTGGAAG TGGAGGTAGA GCCTAGAGAC AGGCCCAGCC GAGAAGCTCC ACCTTGGGAG 
AACACCAGCC TAAGGGGGTT AAACCCCAAG ATCCTCTTTT CCAACAGGGA AGAGCAGCAG 
GACATTCTGT CTAAGTTTGA GGAAAAAGAA CCAGAGACTG GGTTAGACAA TACCTCTCCT 
AAGGAGAGTC AGTCTGTCCT GATTGCAGAC GGAGATGGAA GTGAAGTATC AGATGAAGAA 
GAGGCTTCAT TCCCCAGTGA GGAGAGGAAA CCAGGAGCTG GAGAAGATAC ACCAAGGCTG 
GCTGCTGGGA CCAAACAGCA AGACTTAATA TTTGATGTGG GCATGCTGGC TGCCCCACAG 
GAGCCTGTAC AGCCCGAAGA AGGTGTTGAT CTCCTGGGGC TACACTCTGA GGGGGACTTA 
AGGCCTGCTG CCCCCTTACA GGCTTGTGGG GTCCCATCTA GCAACACTGA CCTGTTGAGC 
TGCCTTCTTG AACCATCTGA TGCTGCTCAA GTAGGGCCTC CTGGTGACCT GCTTGGTGGT 
GAGGCTCCAC TGCTGCTAGC AAGCCCAGTT TCTCCTCTTG GGCTGCAGAA CAACCTACAA 
GGAAAAGTCC CTGACACTGT GGACCCATTT GACCAGTTCC TGCTGTCATC CAACTCAGAC 
ACCCAGCCCT GCTCCAAGCC TGATCTCTTT GGAGAGTTTC TCAACTCTGA CTCTGTAGCT 
TCCTCAACTG CCTTCCCATC CACCCACAGT GCCCCACCCC CATCCTGTAG CACTGCCTTC 
CTGCACCTAG GGGATCTGCC AGCAGAGCCC AGCAAGGTAA TAGCTTCATC CAGCCACCCA 
GATCTGCTAG GAGGATGGGA TACGTGGGCT GACACAGCTA CACCTGGGCC AGCCTCCATT 
CCAGTACCAG AAGGTACCCT CTTCTCTTCT GCAGGTCACC CAGCCCCTCC TGGCCCCAAC 
CCCAGCCAAA CCAAGTCTCA GAACCTTGAC CCATTTGCTG ATCTCAGTGA TCTCAGCTCT 
AGCCTCCAAG GCCTGCCTGC TGGACTTCCT GCAGGGGGCT TTGTTGGCGC ACCAGCCCCC 
ACTCAAAAGA GCAACAGCCC CTGGCAGGCA AATCGTCCCA CAGCCCCTGG AACCTCATGG 
ACCCCCCAGG CCAAGCCAGC CCCCAGAGCC TCTGAACAGC TAAGGTCTCA CTTCAGTGTG 
ATCGGGGCCC GAGAAGAGAG GGGCGTCCGT GTGCCCAGCT TTGCCCAAAA GCCAAAGGTC 
TCAGAAAATG ATTTTGAAGA TCTTCTGCCT AATCAAGGCT TCTCTAAGTC TGACAAGAAG 
GGGCCAAAGA CCATGGCAGA GATGCGGAAA CAGGAACTTG CCAGAGATAC AGACCCACTC 
AAATTGAAGC TTTTGGACTG GATTGAAGGC AAGGAGAGGA ACATTCGTGC ACTGCTGTCC 
ACTCTGCACA CGGTATTGTG GGATGGGGAG AGCCGCTGGA CACCTGTGAG TATGGCTGAC 
CTGGTGACTC CAGAGCAGGT GAAGAAGCAG TACCGCCGTG CAGTGCTGGT AGTGCATCCT 
GATAAGGCCA CAGGGCAGCC GTATGAACAG TATGCCAAGA TGATCTTCAT GGAGCTGAAT 
GATGCATGGT CTGAGTTTGA AAACCAGGGC TCGAGGCCCC TCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gak ( XM_006534905.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006534968.1 Mus musculus cyclin G associated kinase (Gak), transcript variant X1, mRNA.

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CloneID	OMu35450
Clone ID Related Accession (Same CDS sequence)	XM_006534905.3 , XM_006534905.2
Accession Version	XM_006534905.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3771bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	cyclin-G-associated kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006534905.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCTGC TGCAGTCTGC GCTGGACTTC CTGGCGGGCC CTGGTTCTCT TGGCGGAGCT 
GCGGGCCGTG ATCAGAGTGA CTTCGTGGGG CAGACAGTGG AGCTGGGCGA GCTGCGTCTG 
CGGGTGCGGC GGGTCCTGGC CGAGGGAGGG TTTGCTTTTG TTTATGAAGC TCAAGATCTG 
GGAAGTGGCA GAGAGTATGC ATTAAAGCGA TTACTATCCA ATGAAGAGGA AAAGAACAGA 
GCCATCATTC AGGAAGTTTG TTTCTTGAAA AAACTTTCTG GCCACCCCAA TATTGTCCAG 
TTCTGCTCTG CAGCATCCAT AGGAAAAGAG GAGTCGGACA CTGGGCAGGC TGAGTTCCTC 
CTGCTTACGG AGCTTTGTAA AGGACAGCTG GTGGAGTTTC TCAAGAGAGT TGAATGTAAA 
GGTCCTCTGT CCTGCGACAG CATTCTGAAG ATCTTCTACC AGACATGCAG AGCAGTGCAG 
CACATGCACA GGCAGAAACC ACCCATCATC CACAGAGATC TCAAGGTTGA AAACTTACTG 
CTTAGTAACC AGGGGACCAT TAAGCTGTGT GACTTTGGCA GTGCCACAAC CATCTCCCAT 
TATCCTGACT ACAGCTGGAG TGCCCAGAAG CGAGCCATGG TGGAGGAAGA GATCACAAGG 
AACACCACAC CCATGTACAG AACACCAGAA ATTGTAGACC TGTATTCCAA CTTCCCCATT 
GGCGAAAAGC AGGATATCTG GGCACTGGGC TGTATCTTAT ACCTGCTGTG TTTCCGGCAG 
CATCCTTTTG AGGATGGAGC AAAACTTCGG ATAGTCAATG GGAAGTATTC CATTCCTGTG 
AATGACACTC GTTACACAGT CTTCCATGAC CTTATTCGTG CCATGCTAAA GGTCAACCCA 
GAGGAGAGGC TGTCCATTGC TGAAGTGGTC CGACAACTGC AGGAAATTGC AGCAGCTAGG 
AATGTGAACC CCAAAGCTCC CATCACAGAG CTGCTGGAGC AGAATGGTGG CTATGGGAAC 
TCAGGGCCTT CCCGAGCACA ACCACCTTGC GGGGGCACCG TGAACAGCAG TGGAGTTTTG 
GCTCTGGCAG AGTATGACCA GCCCTACGGT GGGTTTCTTG ATATTCTGCG GGGTGGGACC 
GAACGGCTCT TCACCAACCT CAAGGATACT TCCTCCAAGG TCATCCAGTC TGTGGCTAAC 
TATGCAAAGG GTGATCTGGA CATATCTTAC ATCACATCCA GGATTGCAGT GATGTCATTC 
CCAGCAGAAG GTGTGGAGTC TGCAATCAAA AACAATATAG AGGATGTGCG AATGTTTCTG 
GATGCTAAGC ATCCAGGACA TTATGCTGTC TACAACCTTT CTCCAAGGAT ATACCGGGCT 
TCCAAGTTTC ACAACCGGGT CACTGAGTGT GGCTGGGCAG TCAGACGGGC ACCACATCTC 
CACAGTTTGT ATACTCTATG TAGGAGCATG CATGCCTGGC TCCGGGAAGA CCACAGGAAC 
GTCTGTGTTG TGCATTGCAT GGATGGGAGA GCTGCATCTG CTGTGGCAGT CTGTGCATTC 
CTGTGCTTCT GCCGTCTCTT CAGCACTGCA GAGGCTGCTG TGTACATGTT CAGCATGAAG 
CGCTGCCCAC CAGGCATTTG GCCATCCCAC AAAAGGTACA TTGAATATGT ATGTGACATG 
GTGGCAGAGG AGCCCATCAC GCCCCATAGC AAGCCAATGC TGGTGAAATC TGTTGTCATG 
ACCCCCGTGC CACTGTTCAG CAAGCAGAGG AATGGCTGCC GACCGTTCTG TGAGGTCTAC 
GTTGGAGAGG AGCGTGTCAC CACCACGTCG CAGGAATATG ACAGAATGAA GGAATTTAAA 
ATTGAGGATG GCAAGGCTGT CATCCCCCTG GGCGTAACAG TTCAAGGAGA CGTGCTCATC 
ATCATCTACC ATGCCAGGGC CACACTGGGA GGGAGGCTGC AGGCTAAGAT GGCGTCCATG 
AAAATGTTCC AGATCCAGTT CCACACTGGG TTCGTGCCTC GAAACGCAAC CACTGTGAAA 
TTTGCAAAAT ATGACCTGGA TGCTTGTGAT ATTCAAGAGA AGTACCCAGA TCTGTTCCAG 
GTGAACCTGG AAGTGGAGGT AGAGCCTAGA GACAGGCCCA GCCGAGAAGC TCCACCTTGG 
GAGAACACCA GCCTAAGGGG GTTAAACCCC AAGATCCTCT TTTCCAACAG GGAAGAGCAG 
CAGGACATTC TGTCTAAGTT TGAGGAAAAA GAACCAGAGA CTGGGTTAGA CAATACCTCT 
CCTAAGGAGA GTCAGTCTGT CCTGATTGCA GACGGAGATG GAAGTGAAGT ATCAGATGAA 
GAAGAGGCTT CATTCCCCAG TGAGGAGAGG AAACCAGGAG CTGGAGAAGA TACACCAAGG 
CTGGCTGCTG GGACCAAACA GCAAGACTTA ATATTTGATG TGGGCATGCT GGCTGCCCCA 
CAGGAGCCTG TACAGCCCGA AGAAGGTGTT GATCTCCTGG GGCTACACTC TGAGGGGGAC 
TTAAGGCCTG CTGCCCCCTT ACAGGCTTGT GGGGTCCCAT CTAGCAACAC TGACCTGTTG 
AGCTGCCTTC TTGAACCATC TGATGCTGCT CAAGTAGGGC CTCCTGGTGA CCTGCTTGGT 
GGTGAGGCTC CACTGCTGCT AGCAAGCCCA GTTTCTCCTC TTGGGCTGCA GAACAACCTA 
CAAGGAAAAG TCCCTGACAC TGTGGACCCA TTTGACCAGT TCCTGCTGTC ATCCAACTCA 
GACACCCAGC CCTGCTCCAA GCCTGATCTC TTTGGAGAGT TTCTCAACTC TGACTCTGTA 
GCTTCCTCAA CTGCCTTCCC ATCCACCCAC AGTGCCCCAC CCCCATCCTG TAGCACTGCC 
TTCCTGCACC TAGGGGATCT GCCAGCAGAG CCCAGCAAGG TAATAGCTTC ATCCAGCCAC 
CCAGATCTGC TAGGAGGATG GGATACGTGG GCTGACACAG CTACACCTGG GCCAGCCTCC 
ATTCCAGTAC CAGAAGGTAC CCTCTTCTCT TCTGCAGGTC ACCCAGCCCC TCCTGGCCCC 
AACCCCAGCC AAACCAAGTC TCAGAACCTT GACCCATTTG CTGATCTCAG TGATCTCAGC 
TCTAGCCTCC AAGGCCTGCC TGCTGGACTT CCTGCAGGGG GCTTTGTTGG CGCACCAGCC 
CCCACTCAAA AGAGCAACAG CCCCTGGCAG GCAAATCGTC CCACAGCCCC TGGAACCTCA 
TGGACCCCCC AGGCCAAGCC AGCCCCCAGA GCCTCTGAAC AGCTAAGGTC TCACTTCAGT 
GTGATCGGGG CCCGAGAAGA GAGGGGCGTC CGTGTGCCCA GCTTTGCCCA AAAGCCAAAG 
GTCTCAGAAA ATGATTTTGA AGATCTTCTG CCTAATCAAG GCTTCTCTAA GTCTGACAAG 
AAGGGGCCAA AGACCATGGC AGAGATGCGG AAACAGGAAC TTGCCAGAGA TACAGACCCA 
CTCAAATTGA AGCTTTTGGA CTGGATTGAA GGCAAGGAGA GGAACATTCG TGCACTGCTG 
TCCACTCTGC ACACGGTATT GTGGGATGGG GAGAGCCGCT GGACACCTGT GAGTATGGCT 
GACCTGGTGA CTCCAGAGCA GGTGAAGAAG CAGTACCGCC GTGCAGTGCT GGTAGTGCAT 
CCTGATAAGG CCACAGGGCA GCCGTATGAA CAGTATGCCA AGATGATCTT CATGGAGCTG 
AATGATGCAT GGTCTGAGTT TGAAAACCAG GGCTCGAGGC CCCTCTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006534968.1
CDS	132..3902
Translation	MSLLQSALDFLAGPGSLGGAAGRDQSDFVGQTVELGELRLRVRRVLAEGGFAFVYEAQDLGSGREYALKRLLSNEEEKNRAIIQEVCFLKKLSGHPNIVQFCSAASIGKEESDTGQAEFLLLTELCKGQLVEFLKRVECKGPLSCDSILKIFYQTCRAVQHMHRQKPPIIHRDLKVENLLLSNQGTIKLCDFGSATTISHYPDYSWSAQKRAMVEEEITRNTTPMYRTPEIVDLYSNFPIGEKQDIWALGCILYLLCFRQHPFEDGAKLRIVNGKYSIPVNDTRYTVFHDLIRAMLKVNPEERLSIAEVVRQLQEIAAARNVNPKAPITELLEQNGGYGNSGPSRAQPPCGGTVNSSGVLALAEYDQPYGGFLDILRGGTERLFTNLKDTSSKVIQSVANYAKGDLDISYITSRIAVMSFPAEGVESAIKNNIEDVRMFLDAKHPGHYAVYNLSPRIYRASKFHNRVTECGWAVRRAPHLHSLYTLCRSMHAWLREDHRNVCVVHCMDGRAASAVAVCAFLCFCRLFSTAEAAVYMFSMKRCPPGIWPSHKRYIEYVCDMVAEEPITPHSKPMLVKSVVMTPVPLFSKQRNGCRPFCEVYVGEERVTTTSQEYDRMKEFKIEDGKAVIPLGVTVQGDVLIIIYHARATLGGRLQAKMASMKMFQIQFHTGFVPRNATTVKFAKYDLDACDIQEKYPDLFQVNLEVEVEPRDRPSREAPPWENTSLRGLNPKILFSNREEQQDILSKFEEKEPETGLDNTSPKESQSVLIADGDGSEVSDEEEASFPSEERKPGAGEDTPRLAAGTKQQDLIFDVGMLAAPQEPVQPEEGVDLLGLHSEGDLRPAAPLQACGVPSSNTDLLSCLLEPSDAAQVGPPGDLLGGEAPLLLASPVSPLGLQNNLQGKVPDTVDPFDQFLLSSNSDTQPCSKPDLFGEFLNSDSVASSTAFPSTHSAPPPSCSTAFLHLGDLPAEPSKVIASSSHPDLLGGWDTWADTATPGPASIPVPEGTLFSSAGHPAPPGPNPSQTKSQNLDPFADLSDLSSSLQGLPAGLPAGGFVGAPAPTQKSNSPWQANRPTAPGTSWTPQAKPAPRASEQLRSHFSVIGAREERGVRVPSFAQKPKVSENDFEDLLPNQGFSKSDKKGPKTMAEMRKQELARDTDPLKLKLLDWIEGKERNIRALLSTLHTVLWDGESRWTPVSMADLVTPEQVKKQYRRAVLVVHPDKATGQPYEQYAKMIFMELNDAWSEFENQGSRPLF

Target ORF information:

RefSeq Version	XM_006534905.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus cyclin G associated kinase (Gak), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006534905.3

ORF Insert Sequence:

ATGTCGCTGC TGCAGTCTGC GCTGGACTTC CTGGCGGGCC CTGGTTCTCT TGGCGGAGCT 
GCGGGCCGTG ATCAGAGTGA CTTCGTGGGG CAGACAGTGG AGCTGGGCGA GCTGCGTCTG 
CGGGTGCGGC GGGTCCTGGC CGAGGGAGGG TTTGCTTTTG TTTATGAAGC TCAAGATCTG 
GGAAGTGGCA GAGAGTATGC ATTAAAGCGA TTACTATCCA ATGAAGAGGA AAAGAACAGA 
GCCATCATTC AGGAAGTTTG TTTCTTGAAA AAACTTTCTG GCCACCCCAA TATTGTCCAG 
TTCTGCTCTG CAGCATCCAT AGGAAAAGAG GAGTCGGACA CTGGGCAGGC TGAGTTCCTC 
CTGCTTACGG AGCTTTGTAA AGGACAGCTG GTGGAGTTTC TCAAGAGAGT TGAATGTAAA 
GGTCCTCTGT CCTGCGACAG CATTCTGAAG ATCTTCTACC AGACATGCAG AGCAGTGCAG 
CACATGCACA GGCAGAAACC ACCCATCATC CACAGAGATC TCAAGGTTGA AAACTTACTG 
CTTAGTAACC AGGGGACCAT TAAGCTGTGT GACTTTGGCA GTGCCACAAC CATCTCCCAT 
TATCCTGACT ACAGCTGGAG TGCCCAGAAG CGAGCCATGG TGGAGGAAGA GATCACAAGG 
AACACCACAC CCATGTACAG AACACCAGAA ATTGTAGACC TGTATTCCAA CTTCCCCATT 
GGCGAAAAGC AGGATATCTG GGCACTGGGC TGTATCTTAT ACCTGCTGTG TTTCCGGCAG 
CATCCTTTTG AGGATGGAGC AAAACTTCGG ATAGTCAATG GGAAGTATTC CATTCCTGTG 
AATGACACTC GTTACACAGT CTTCCATGAC CTTATTCGTG CCATGCTAAA GGTCAACCCA 
GAGGAGAGGC TGTCCATTGC TGAAGTGGTC CGACAACTGC AGGAAATTGC AGCAGCTAGG 
AATGTGAACC CCAAAGCTCC CATCACAGAG CTGCTGGAGC AGAATGGTGG CTATGGGAAC 
TCAGGGCCTT CCCGAGCACA ACCACCTTGC GGGGGCACCG TGAACAGCAG TGGAGTTTTG 
GCTCTGGCAG AGTATGACCA GCCCTACGGT GGGTTTCTTG ATATTCTGCG GGGTGGGACC 
GAACGGCTCT TCACCAACCT CAAGGATACT TCCTCCAAGG TCATCCAGTC TGTGGCTAAC 
TATGCAAAGG GTGATCTGGA CATATCTTAC ATCACATCCA GGATTGCAGT GATGTCATTC 
CCAGCAGAAG GTGTGGAGTC TGCAATCAAA AACAATATAG AGGATGTGCG AATGTTTCTG 
GATGCTAAGC ATCCAGGACA TTATGCTGTC TACAACCTTT CTCCAAGGAT ATACCGGGCT 
TCCAAGTTTC ACAACCGGGT CACTGAGTGT GGCTGGGCAG TCAGACGGGC ACCACATCTC 
CACAGTTTGT ATACTCTATG TAGGAGCATG CATGCCTGGC TCCGGGAAGA CCACAGGAAC 
GTCTGTGTTG TGCATTGCAT GGATGGGAGA GCTGCATCTG CTGTGGCAGT CTGTGCATTC 
CTGTGCTTCT GCCGTCTCTT CAGCACTGCA GAGGCTGCTG TGTACATGTT CAGCATGAAG 
CGCTGCCCAC CAGGCATTTG GCCATCCCAC AAAAGGTACA TTGAATATGT ATGTGACATG 
GTGGCAGAGG AGCCCATCAC GCCCCATAGC AAGCCAATGC TGGTGAAATC TGTTGTCATG 
ACCCCCGTGC CACTGTTCAG CAAGCAGAGG AATGGCTGCC GACCGTTCTG TGAGGTCTAC 
GTTGGAGAGG AGCGTGTCAC CACCACGTCG CAGGAATATG ACAGAATGAA GGAATTTAAA 
ATTGAGGATG GCAAGGCTGT CATCCCCCTG GGCGTAACAG TTCAAGGAGA CGTGCTCATC 
ATCATCTACC ATGCCAGGGC CACACTGGGA GGGAGGCTGC AGGCTAAGAT GGCGTCCATG 
AAAATGTTCC AGATCCAGTT CCACACTGGG TTCGTGCCTC GAAACGCAAC CACTGTGAAA 
TTTGCAAAAT ATGACCTGGA TGCTTGTGAT ATTCAAGAGA AGTACCCAGA TCTGTTCCAG 
GTGAACCTGG AAGTGGAGGT AGAGCCTAGA GACAGGCCCA GCCGAGAAGC TCCACCTTGG 
GAGAACACCA GCCTAAGGGG GTTAAACCCC AAGATCCTCT TTTCCAACAG GGAAGAGCAG 
CAGGACATTC TGTCTAAGTT TGAGGAAAAA GAACCAGAGA CTGGGTTAGA CAATACCTCT 
CCTAAGGAGA GTCAGTCTGT CCTGATTGCA GACGGAGATG GAAGTGAAGT ATCAGATGAA 
GAAGAGGCTT CATTCCCCAG TGAGGAGAGG AAACCAGGAG CTGGAGAAGA TACACCAAGG 
CTGGCTGCTG GGACCAAACA GCAAGACTTA ATATTTGATG TGGGCATGCT GGCTGCCCCA 
CAGGAGCCTG TACAGCCCGA AGAAGGTGTT GATCTCCTGG GGCTACACTC TGAGGGGGAC 
TTAAGGCCTG CTGCCCCCTT ACAGGCTTGT GGGGTCCCAT CTAGCAACAC TGACCTGTTG 
AGCTGCCTTC TTGAACCATC TGATGCTGCT CAAGTAGGGC CTCCTGGTGA CCTGCTTGGT 
GGTGAGGCTC CACTGCTGCT AGCAAGCCCA GTTTCTCCTC TTGGGCTGCA GAACAACCTA 
CAAGGAAAAG TCCCTGACAC TGTGGACCCA TTTGACCAGT TCCTGCTGTC ATCCAACTCA 
GACACCCAGC CCTGCTCCAA GCCTGATCTC TTTGGAGAGT TTCTCAACTC TGACTCTGTA 
GCTTCCTCAA CTGCCTTCCC ATCCACCCAC AGTGCCCCAC CCCCATCCTG TAGCACTGCC 
TTCCTGCACC TAGGGGATCT GCCAGCAGAG CCCAGCAAGG TAATAGCTTC ATCCAGCCAC 
CCAGATCTGC TAGGAGGATG GGATACGTGG GCTGACACAG CTACACCTGG GCCAGCCTCC 
ATTCCAGTAC CAGAAGGTAC CCTCTTCTCT TCTGCAGGTC ACCCAGCCCC TCCTGGCCCC 
AACCCCAGCC AAACCAAGTC TCAGAACCTT GACCCATTTG CTGATCTCAG TGATCTCAGC 
TCTAGCCTCC AAGGCCTGCC TGCTGGACTT CCTGCAGGGG GCTTTGTTGG CGCACCAGCC 
CCCACTCAAA AGAGCAACAG CCCCTGGCAG GCAAATCGTC CCACAGCCCC TGGAACCTCA 
TGGACCCCCC AGGCCAAGCC AGCCCCCAGA GCCTCTGAAC AGCTAAGGTC TCACTTCAGT 
GTGATCGGGG CCCGAGAAGA GAGGGGCGTC CGTGTGCCCA GCTTTGCCCA AAAGCCAAAG 
GTCTCAGAAA ATGATTTTGA AGATCTTCTG CCTAATCAAG GCTTCTCTAA GTCTGACAAG 
AAGGGGCCAA AGACCATGGC AGAGATGCGG AAACAGGAAC TTGCCAGAGA TACAGACCCA 
CTCAAATTGA AGCTTTTGGA CTGGATTGAA GGCAAGGAGA GGAACATTCG TGCACTGCTG 
TCCACTCTGC ACACGGTATT GTGGGATGGG GAGAGCCGCT GGACACCTGT GAGTATGGCT 
GACCTGGTGA CTCCAGAGCA GGTGAAGAAG CAGTACCGCC GTGCAGTGCT GGTAGTGCAT 
CCTGATAAGG CCACAGGGCA GCCGTATGAA CAGTATGCCA AGATGATCTT CATGGAGCTG 
AATGATGCAT GGTCTGAGTT TGAAAACCAG GGCTCGAGGC CCCTCTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gak ( XM_017320853.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017176342.1 Mus musculus cyclin G associated kinase (Gak), transcript variant X4, mRNA.

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CloneID	OMu81362
Clone ID Related Accession (Same CDS sequence)	XM_017320853.1 , NM_001359922.1
Accession Version	XM_017320853.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3288bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	cyclin-G-associated kinase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACAGGC AGAAACCACC CATCATCCAC AGAGATCTCA AGGTTGAAAA CTTACTGCTT 
AGTAACCAGG GGACCATTAA GCTGTGTGAC TTTGGCAGTG CCACAACCAT CTCCCATTAT 
CCTGACTACA GCTGGAGTGC CCAGAAGCGA GCCATGGTGG AGGAAGAGAT CACAAGGAAC 
ACCACACCCA TGTACAGAAC ACCAGAAATT GTAGACCTGT ATTCCAACTT CCCCATTGGC 
GAAAAGCAGG ATATCTGGGC ACTGGGCTGT ATCTTATACC TGCTGTGTTT CCGGCAGCAT 
CCTTTTGAGG ATGGAGCAAA ACTTCGGATA GTCAATGGGA AGTATTCCAT TCCTGTGAAT 
GACACTCGTT ACACAGTCTT CCATGACCTT ATTCGTGCCA TGCTAAAGGT CAACCCAGAG 
GAGAGGCTGT CCATTGCTGA AGTGGTCCGA CAACTGCAGG AAATTGCAGC AGCTAGGAAT 
GTGAACCCCA AAGCTCCCAT CACAGAGCTG CTGGAGCAGA ATGGTGGCTA TGGGAACTCA 
GGGCCTTCCC GAGCACAACC ACCTTGCGGG GGCACCGTGA ACAGCAGTGG AGTTTTGGCT 
CTGGCAGAGT ATGACCAGCC CTACGGTGGG TTTCTTGATA TTCTGCGGGG TGGGACCGAA 
CGGCTCTTCA CCAACCTCAA GGATACTTCC TCCAAGGTCA TCCAGTCTGT GGCTAACTAT 
GCAAAGGGTG ATCTGGACAT ATCTTACATC ACATCCAGGA TTGCAGTGAT GTCATTCCCA 
GCAGAAGGTG TGGAGTCTGC AATCAAAAAC AATATAGAGG ATGTGCGAAT GTTTCTGGAT 
GCTAAGCATC CAGGACATTA TGCTGTCTAC AACCTTTCTC CAAGGATATA CCGGGCTTCC 
AAGTTTCACA ACCGGGTCAC TGAGTGTGGC TGGGCAGTCA GACGGGCACC ACATCTCCAC 
AGTTTGTATA CTCTATGTAG GAGCATGCAT GCCTGGCTCC GGGAAGACCA CAGGAACGTC 
TGTGTTGTGC ATTGCATGGA TGGGAGAGCT GCATCTGCTG TGGCAGTCTG TGCATTCCTG 
TGCTTCTGCC GTCTCTTCAG CACTGCAGAG GCTGCTGTGT ACATGTTCAG CATGAAGCGC 
TGCCCACCAG GCATTTGGCC ATCCCACAAA AGGTACATTG AATATGTATG TGACATGGTG 
GCAGAGGAGC CCATCACGCC CCATAGCAAG CCAATGCTGG TGAAATCTGT TGTCATGACC 
CCCGTGCCAC TGTTCAGCAA GCAGAGGAAT GGCTGCCGAC CGTTCTGTGA GGTCTACGTT 
GGAGAGGAGC GTGTCACCAC CACGTCGCAG GAATATGACA GAATGAAGGA ATTTAAAATT 
GAGGATGGCA AGGCTGTCAT CCCCCTGGGC GTAACAGTTC AAGGAGACGT GCTCATCATC 
ATCTACCATG CCAGGGCCAC ACTGGGAGGG AGGCTGCAGG CTAAGATGGC GTCCATGAAA 
ATGTTCCAGA TCCAGTTCCA CACTGGGTTC GTGCCTCGAA ACGCAACCAC TGTGAAATTT 
GCAAAATATG ACCTGGATGC TTGTGATATT CAAGAGAAGT ACCCAGATCT GTTCCAGGTG 
AACCTGGAAG TGGAGGTAGA GCCTAGAGAC AGGCCCAGCC GAGAAGCTCC ACCTTGGGAG 
AACACCAGCC TAAGGGGGTT AAACCCCAAG ATCCTCTTTT CCAACAGGGA AGAGCAGCAG 
GACATTCTGT CTAAGTTTGA GGAAAAAGAA CCAGAGACTG GGTTAGACAA TACCTCTCCT 
AAGGAGAGTC AGTCTGTCCT GATTGCAGAC GGAGATGGAA GTGAAGTATC AGATGAAGAA 
GAGGCTTCAT TCCCCAGTGA GGAGAGGAAA CCAGGAGCTG GAGAAGATAC ACCAAGGCTG 
GCTGCTGGGA CCAAACAGCA AGACTTAATA TTTGATGTGG GCATGCTGGC TGCCCCACAG 
GAGCCTGTAC AGCCCGAAGA AGGTGTTGAT CTCCTGGGGC TACACTCTGA GGGGGACTTA 
AGGCCTGCTG CCCCCTTACA GGCTTGTGGG GTCCCATCTA GCAACACTGA CCTGTTGAGC 
TGCCTTCTTG AACCATCTGA TGCTGCTCAA GTAGGGCCTC CTGGTGACCT GCTTGGTGGT 
GAGGCTCCAC TGCTGCTAGC AAGCCCAGTT TCTCCTCTTG GGCTGCAGAA CAACCTACAA 
GGAAAAGTCC CTGACACTGT GGACCCATTT GACCAGTTCC TGCTGTCATC CAACTCAGAC 
ACCCAGCCCT GCTCCAAGCC TGATCTCTTT GGAGAGTTTC TCAACTCTGA CTCTGTAGCT 
TCCTCAACTG CCTTCCCATC CACCCACAGT GCCCCACCCC CATCCTGTAG CACTGCCTTC 
CTGCACCTAG GGGATCTGCC AGCAGAGCCC AGCAAGGTAA TAGCTTCATC CAGCCACCCA 
GATCTGCTAG GAGGATGGGA TACGTGGGCT GACACAGCTA CACCTGGGCC AGCCTCCATT 
CCAGTACCAG AAGGTACCCT CTTCTCTTCT GCAGGTCACC CAGCCCCTCC TGGCCCCAAC 
CCCAGCCAAA CCAAGTCTCA GAACCTTGAC CCATTTGCTG ATCTCAGTGA TCTCAGCTCT 
AGCCTCCAAG GCCTGCCTGC TGGACTTCCT GCAGGGGGCT TTGTTGGCGC ACCAGCCCCC 
ACTCAAAAGA GCAACAGCCC CTGGCAGGCA AATCGTCCCA CAGCCCCTGG AACCTCATGG 
ACCCCCCAGG CCAAGCCAGC CCCCAGAGCC TCTGAACAGC TAAGGTCTCA CTTCAGTGTG 
ATCGGGGCCC GAGAAGAGAG GGGCGTCCGT GTGCCCAGCT TTGCCCAAAA GCCAAAGGTC 
TCAGAAAATG ATTTTGAAGA TCTTCTGCCT AATCAAGGCT TCTCTAAGTC TGACAAGAAG 
GGGCCAAAGA CCATGGCAGA GATGCGGAAA CAGGAACTTG CCAGAGATAC AGACCCACTC 
AAATTGAAGC TTTTGGACTG GATTGAAGGC AAGGAGAGGA ACATTCGTGC ACTGCTGTCC 
ACTCTGCACA CGGTATTGTG GGATGGGGAG AGCCGCTGGA CACCTGTGAG TATGGCTGAC 
CTGGTGACTC CAGAGCAGGT GAAGAAGCAG TACCGCCGTG CAGTGCTGGT AGTGCATCCT 
GATAAGGCCA CAGGGCAGCC GTATGAACAG TATGCCAAGA TGATCTTCAT GGAGCTGAAT 
GATGCATGGT CTGAGTTTGA AAACCAGGGC TCGAGGCCCC TCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017176342.1
CDS	497..3784
Translation	MHRQKPPIIHRDLKVENLLLSNQGTIKLCDFGSATTISHYPDYSWSAQKRAMVEEEITRNTTPMYRTPEIVDLYSNFPIGEKQDIWALGCILYLLCFRQHPFEDGAKLRIVNGKYSIPVNDTRYTVFHDLIRAMLKVNPEERLSIAEVVRQLQEIAAARNVNPKAPITELLEQNGGYGNSGPSRAQPPCGGTVNSSGVLALAEYDQPYGGFLDILRGGTERLFTNLKDTSSKVIQSVANYAKGDLDISYITSRIAVMSFPAEGVESAIKNNIEDVRMFLDAKHPGHYAVYNLSPRIYRASKFHNRVTECGWAVRRAPHLHSLYTLCRSMHAWLREDHRNVCVVHCMDGRAASAVAVCAFLCFCRLFSTAEAAVYMFSMKRCPPGIWPSHKRYIEYVCDMVAEEPITPHSKPMLVKSVVMTPVPLFSKQRNGCRPFCEVYVGEERVTTTSQEYDRMKEFKIEDGKAVIPLGVTVQGDVLIIIYHARATLGGRLQAKMASMKMFQIQFHTGFVPRNATTVKFAKYDLDACDIQEKYPDLFQVNLEVEVEPRDRPSREAPPWENTSLRGLNPKILFSNREEQQDILSKFEEKEPETGLDNTSPKESQSVLIADGDGSEVSDEEEASFPSEERKPGAGEDTPRLAAGTKQQDLIFDVGMLAAPQEPVQPEEGVDLLGLHSEGDLRPAAPLQACGVPSSNTDLLSCLLEPSDAAQVGPPGDLLGGEAPLLLASPVSPLGLQNNLQGKVPDTVDPFDQFLLSSNSDTQPCSKPDLFGEFLNSDSVASSTAFPSTHSAPPPSCSTAFLHLGDLPAEPSKVIASSSHPDLLGGWDTWADTATPGPASIPVPEGTLFSSAGHPAPPGPNPSQTKSQNLDPFADLSDLSSSLQGLPAGLPAGGFVGAPAPTQKSNSPWQANRPTAPGTSWTPQAKPAPRASEQLRSHFSVIGAREERGVRVPSFAQKPKVSENDFEDLLPNQGFSKSDKKGPKTMAEMRKQELARDTDPLKLKLLDWIEGKERNIRALLSTLHTVLWDGESRWTPVSMADLVTPEQVKKQYRRAVLVVHPDKATGQPYEQYAKMIFMELNDAWSEFENQGSRPLF

Target ORF information:

RefSeq Version	XM_017320853.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus cyclin G associated kinase (Gak), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017320853.1

ORF Insert Sequence:

ATGCACAGGC AGAAACCACC CATCATCCAC AGAGATCTCA AGGTTGAAAA CTTACTGCTT 
AGTAACCAGG GGACCATTAA GCTGTGTGAC TTTGGCAGTG CCACAACCAT CTCCCATTAT 
CCTGACTACA GCTGGAGTGC CCAGAAGCGA GCCATGGTGG AGGAAGAGAT CACAAGGAAC 
ACCACACCCA TGTACAGAAC ACCAGAAATT GTAGACCTGT ATTCCAACTT CCCCATTGGC 
GAAAAGCAGG ATATCTGGGC ACTGGGCTGT ATCTTATACC TGCTGTGTTT CCGGCAGCAT 
CCTTTTGAGG ATGGAGCAAA ACTTCGGATA GTCAATGGGA AGTATTCCAT TCCTGTGAAT 
GACACTCGTT ACACAGTCTT CCATGACCTT ATTCGTGCCA TGCTAAAGGT CAACCCAGAG 
GAGAGGCTGT CCATTGCTGA AGTGGTCCGA CAACTGCAGG AAATTGCAGC AGCTAGGAAT 
GTGAACCCCA AAGCTCCCAT CACAGAGCTG CTGGAGCAGA ATGGTGGCTA TGGGAACTCA 
GGGCCTTCCC GAGCACAACC ACCTTGCGGG GGCACCGTGA ACAGCAGTGG AGTTTTGGCT 
CTGGCAGAGT ATGACCAGCC CTACGGTGGG TTTCTTGATA TTCTGCGGGG TGGGACCGAA 
CGGCTCTTCA CCAACCTCAA GGATACTTCC TCCAAGGTCA TCCAGTCTGT GGCTAACTAT 
GCAAAGGGTG ATCTGGACAT ATCTTACATC ACATCCAGGA TTGCAGTGAT GTCATTCCCA 
GCAGAAGGTG TGGAGTCTGC AATCAAAAAC AATATAGAGG ATGTGCGAAT GTTTCTGGAT 
GCTAAGCATC CAGGACATTA TGCTGTCTAC AACCTTTCTC CAAGGATATA CCGGGCTTCC 
AAGTTTCACA ACCGGGTCAC TGAGTGTGGC TGGGCAGTCA GACGGGCACC ACATCTCCAC 
AGTTTGTATA CTCTATGTAG GAGCATGCAT GCCTGGCTCC GGGAAGACCA CAGGAACGTC 
TGTGTTGTGC ATTGCATGGA TGGGAGAGCT GCATCTGCTG TGGCAGTCTG TGCATTCCTG 
TGCTTCTGCC GTCTCTTCAG CACTGCAGAG GCTGCTGTGT ACATGTTCAG CATGAAGCGC 
TGCCCACCAG GCATTTGGCC ATCCCACAAA AGGTACATTG AATATGTATG TGACATGGTG 
GCAGAGGAGC CCATCACGCC CCATAGCAAG CCAATGCTGG TGAAATCTGT TGTCATGACC 
CCCGTGCCAC TGTTCAGCAA GCAGAGGAAT GGCTGCCGAC CGTTCTGTGA GGTCTACGTT 
GGAGAGGAGC GTGTCACCAC CACGTCGCAG GAATATGACA GAATGAAGGA ATTTAAAATT 
GAGGATGGCA AGGCTGTCAT CCCCCTGGGC GTAACAGTTC AAGGAGACGT GCTCATCATC 
ATCTACCATG CCAGGGCCAC ACTGGGAGGG AGGCTGCAGG CTAAGATGGC GTCCATGAAA 
ATGTTCCAGA TCCAGTTCCA CACTGGGTTC GTGCCTCGAA ACGCAACCAC TGTGAAATTT 
GCAAAATATG ACCTGGATGC TTGTGATATT CAAGAGAAGT ACCCAGATCT GTTCCAGGTG 
AACCTGGAAG TGGAGGTAGA GCCTAGAGAC AGGCCCAGCC GAGAAGCTCC ACCTTGGGAG 
AACACCAGCC TAAGGGGGTT AAACCCCAAG ATCCTCTTTT CCAACAGGGA AGAGCAGCAG 
GACATTCTGT CTAAGTTTGA GGAAAAAGAA CCAGAGACTG GGTTAGACAA TACCTCTCCT 
AAGGAGAGTC AGTCTGTCCT GATTGCAGAC GGAGATGGAA GTGAAGTATC AGATGAAGAA 
GAGGCTTCAT TCCCCAGTGA GGAGAGGAAA CCAGGAGCTG GAGAAGATAC ACCAAGGCTG 
GCTGCTGGGA CCAAACAGCA AGACTTAATA TTTGATGTGG GCATGCTGGC TGCCCCACAG 
GAGCCTGTAC AGCCCGAAGA AGGTGTTGAT CTCCTGGGGC TACACTCTGA GGGGGACTTA 
AGGCCTGCTG CCCCCTTACA GGCTTGTGGG GTCCCATCTA GCAACACTGA CCTGTTGAGC 
TGCCTTCTTG AACCATCTGA TGCTGCTCAA GTAGGGCCTC CTGGTGACCT GCTTGGTGGT 
GAGGCTCCAC TGCTGCTAGC AAGCCCAGTT TCTCCTCTTG GGCTGCAGAA CAACCTACAA 
GGAAAAGTCC CTGACACTGT GGACCCATTT GACCAGTTCC TGCTGTCATC CAACTCAGAC 
ACCCAGCCCT GCTCCAAGCC TGATCTCTTT GGAGAGTTTC TCAACTCTGA CTCTGTAGCT 
TCCTCAACTG CCTTCCCATC CACCCACAGT GCCCCACCCC CATCCTGTAG CACTGCCTTC 
CTGCACCTAG GGGATCTGCC AGCAGAGCCC AGCAAGGTAA TAGCTTCATC CAGCCACCCA 
GATCTGCTAG GAGGATGGGA TACGTGGGCT GACACAGCTA CACCTGGGCC AGCCTCCATT 
CCAGTACCAG AAGGTACCCT CTTCTCTTCT GCAGGTCACC CAGCCCCTCC TGGCCCCAAC 
CCCAGCCAAA CCAAGTCTCA GAACCTTGAC CCATTTGCTG ATCTCAGTGA TCTCAGCTCT 
AGCCTCCAAG GCCTGCCTGC TGGACTTCCT GCAGGGGGCT TTGTTGGCGC ACCAGCCCCC 
ACTCAAAAGA GCAACAGCCC CTGGCAGGCA AATCGTCCCA CAGCCCCTGG AACCTCATGG 
ACCCCCCAGG CCAAGCCAGC CCCCAGAGCC TCTGAACAGC TAAGGTCTCA CTTCAGTGTG 
ATCGGGGCCC GAGAAGAGAG GGGCGTCCGT GTGCCCAGCT TTGCCCAAAA GCCAAAGGTC 
TCAGAAAATG ATTTTGAAGA TCTTCTGCCT AATCAAGGCT TCTCTAAGTC TGACAAGAAG 
GGGCCAAAGA CCATGGCAGA GATGCGGAAA CAGGAACTTG CCAGAGATAC AGACCCACTC 
AAATTGAAGC TTTTGGACTG GATTGAAGGC AAGGAGAGGA ACATTCGTGC ACTGCTGTCC 
ACTCTGCACA CGGTATTGTG GGATGGGGAG AGCCGCTGGA CACCTGTGAG TATGGCTGAC 
CTGGTGACTC CAGAGCAGGT GAAGAAGCAG TACCGCCGTG CAGTGCTGGT AGTGCATCCT 
GATAAGGCCA CAGGGCAGCC GTATGAACAG TATGCCAAGA TGATCTTCAT GGAGCTGAAT 
GATGCATGGT CTGAGTTTGA AAACCAGGGC TCGAGGCCCC TCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gak ( XM_006534905.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006534968.1 Mus musculus cyclin G associated kinase (Gak), transcript variant X1, mRNA.

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CloneID	OMu35450
Clone ID Related Accession (Same CDS sequence)	XM_006534905.3 , XM_006534905.2
Accession Version	XM_006534905.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3771bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	cyclin-G-associated kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006534905.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCTGC TGCAGTCTGC GCTGGACTTC CTGGCGGGCC CTGGTTCTCT TGGCGGAGCT 
GCGGGCCGTG ATCAGAGTGA CTTCGTGGGG CAGACAGTGG AGCTGGGCGA GCTGCGTCTG 
CGGGTGCGGC GGGTCCTGGC CGAGGGAGGG TTTGCTTTTG TTTATGAAGC TCAAGATCTG 
GGAAGTGGCA GAGAGTATGC ATTAAAGCGA TTACTATCCA ATGAAGAGGA AAAGAACAGA 
GCCATCATTC AGGAAGTTTG TTTCTTGAAA AAACTTTCTG GCCACCCCAA TATTGTCCAG 
TTCTGCTCTG CAGCATCCAT AGGAAAAGAG GAGTCGGACA CTGGGCAGGC TGAGTTCCTC 
CTGCTTACGG AGCTTTGTAA AGGACAGCTG GTGGAGTTTC TCAAGAGAGT TGAATGTAAA 
GGTCCTCTGT CCTGCGACAG CATTCTGAAG ATCTTCTACC AGACATGCAG AGCAGTGCAG 
CACATGCACA GGCAGAAACC ACCCATCATC CACAGAGATC TCAAGGTTGA AAACTTACTG 
CTTAGTAACC AGGGGACCAT TAAGCTGTGT GACTTTGGCA GTGCCACAAC CATCTCCCAT 
TATCCTGACT ACAGCTGGAG TGCCCAGAAG CGAGCCATGG TGGAGGAAGA GATCACAAGG 
AACACCACAC CCATGTACAG AACACCAGAA ATTGTAGACC TGTATTCCAA CTTCCCCATT 
GGCGAAAAGC AGGATATCTG GGCACTGGGC TGTATCTTAT ACCTGCTGTG TTTCCGGCAG 
CATCCTTTTG AGGATGGAGC AAAACTTCGG ATAGTCAATG GGAAGTATTC CATTCCTGTG 
AATGACACTC GTTACACAGT CTTCCATGAC CTTATTCGTG CCATGCTAAA GGTCAACCCA 
GAGGAGAGGC TGTCCATTGC TGAAGTGGTC CGACAACTGC AGGAAATTGC AGCAGCTAGG 
AATGTGAACC CCAAAGCTCC CATCACAGAG CTGCTGGAGC AGAATGGTGG CTATGGGAAC 
TCAGGGCCTT CCCGAGCACA ACCACCTTGC GGGGGCACCG TGAACAGCAG TGGAGTTTTG 
GCTCTGGCAG AGTATGACCA GCCCTACGGT GGGTTTCTTG ATATTCTGCG GGGTGGGACC 
GAACGGCTCT TCACCAACCT CAAGGATACT TCCTCCAAGG TCATCCAGTC TGTGGCTAAC 
TATGCAAAGG GTGATCTGGA CATATCTTAC ATCACATCCA GGATTGCAGT GATGTCATTC 
CCAGCAGAAG GTGTGGAGTC TGCAATCAAA AACAATATAG AGGATGTGCG AATGTTTCTG 
GATGCTAAGC ATCCAGGACA TTATGCTGTC TACAACCTTT CTCCAAGGAT ATACCGGGCT 
TCCAAGTTTC ACAACCGGGT CACTGAGTGT GGCTGGGCAG TCAGACGGGC ACCACATCTC 
CACAGTTTGT ATACTCTATG TAGGAGCATG CATGCCTGGC TCCGGGAAGA CCACAGGAAC 
GTCTGTGTTG TGCATTGCAT GGATGGGAGA GCTGCATCTG CTGTGGCAGT CTGTGCATTC 
CTGTGCTTCT GCCGTCTCTT CAGCACTGCA GAGGCTGCTG TGTACATGTT CAGCATGAAG 
CGCTGCCCAC CAGGCATTTG GCCATCCCAC AAAAGGTACA TTGAATATGT ATGTGACATG 
GTGGCAGAGG AGCCCATCAC GCCCCATAGC AAGCCAATGC TGGTGAAATC TGTTGTCATG 
ACCCCCGTGC CACTGTTCAG CAAGCAGAGG AATGGCTGCC GACCGTTCTG TGAGGTCTAC 
GTTGGAGAGG AGCGTGTCAC CACCACGTCG CAGGAATATG ACAGAATGAA GGAATTTAAA 
ATTGAGGATG GCAAGGCTGT CATCCCCCTG GGCGTAACAG TTCAAGGAGA CGTGCTCATC 
ATCATCTACC ATGCCAGGGC CACACTGGGA GGGAGGCTGC AGGCTAAGAT GGCGTCCATG 
AAAATGTTCC AGATCCAGTT CCACACTGGG TTCGTGCCTC GAAACGCAAC CACTGTGAAA 
TTTGCAAAAT ATGACCTGGA TGCTTGTGAT ATTCAAGAGA AGTACCCAGA TCTGTTCCAG 
GTGAACCTGG AAGTGGAGGT AGAGCCTAGA GACAGGCCCA GCCGAGAAGC TCCACCTTGG 
GAGAACACCA GCCTAAGGGG GTTAAACCCC AAGATCCTCT TTTCCAACAG GGAAGAGCAG 
CAGGACATTC TGTCTAAGTT TGAGGAAAAA GAACCAGAGA CTGGGTTAGA CAATACCTCT 
CCTAAGGAGA GTCAGTCTGT CCTGATTGCA GACGGAGATG GAAGTGAAGT ATCAGATGAA 
GAAGAGGCTT CATTCCCCAG TGAGGAGAGG AAACCAGGAG CTGGAGAAGA TACACCAAGG 
CTGGCTGCTG GGACCAAACA GCAAGACTTA ATATTTGATG TGGGCATGCT GGCTGCCCCA 
CAGGAGCCTG TACAGCCCGA AGAAGGTGTT GATCTCCTGG GGCTACACTC TGAGGGGGAC 
TTAAGGCCTG CTGCCCCCTT ACAGGCTTGT GGGGTCCCAT CTAGCAACAC TGACCTGTTG 
AGCTGCCTTC TTGAACCATC TGATGCTGCT CAAGTAGGGC CTCCTGGTGA CCTGCTTGGT 
GGTGAGGCTC CACTGCTGCT AGCAAGCCCA GTTTCTCCTC TTGGGCTGCA GAACAACCTA 
CAAGGAAAAG TCCCTGACAC TGTGGACCCA TTTGACCAGT TCCTGCTGTC ATCCAACTCA 
GACACCCAGC CCTGCTCCAA GCCTGATCTC TTTGGAGAGT TTCTCAACTC TGACTCTGTA 
GCTTCCTCAA CTGCCTTCCC ATCCACCCAC AGTGCCCCAC CCCCATCCTG TAGCACTGCC 
TTCCTGCACC TAGGGGATCT GCCAGCAGAG CCCAGCAAGG TAATAGCTTC ATCCAGCCAC 
CCAGATCTGC TAGGAGGATG GGATACGTGG GCTGACACAG CTACACCTGG GCCAGCCTCC 
ATTCCAGTAC CAGAAGGTAC CCTCTTCTCT TCTGCAGGTC ACCCAGCCCC TCCTGGCCCC 
AACCCCAGCC AAACCAAGTC TCAGAACCTT GACCCATTTG CTGATCTCAG TGATCTCAGC 
TCTAGCCTCC AAGGCCTGCC TGCTGGACTT CCTGCAGGGG GCTTTGTTGG CGCACCAGCC 
CCCACTCAAA AGAGCAACAG CCCCTGGCAG GCAAATCGTC CCACAGCCCC TGGAACCTCA 
TGGACCCCCC AGGCCAAGCC AGCCCCCAGA GCCTCTGAAC AGCTAAGGTC TCACTTCAGT 
GTGATCGGGG CCCGAGAAGA GAGGGGCGTC CGTGTGCCCA GCTTTGCCCA AAAGCCAAAG 
GTCTCAGAAA ATGATTTTGA AGATCTTCTG CCTAATCAAG GCTTCTCTAA GTCTGACAAG 
AAGGGGCCAA AGACCATGGC AGAGATGCGG AAACAGGAAC TTGCCAGAGA TACAGACCCA 
CTCAAATTGA AGCTTTTGGA CTGGATTGAA GGCAAGGAGA GGAACATTCG TGCACTGCTG 
TCCACTCTGC ACACGGTATT GTGGGATGGG GAGAGCCGCT GGACACCTGT GAGTATGGCT 
GACCTGGTGA CTCCAGAGCA GGTGAAGAAG CAGTACCGCC GTGCAGTGCT GGTAGTGCAT 
CCTGATAAGG CCACAGGGCA GCCGTATGAA CAGTATGCCA AGATGATCTT CATGGAGCTG 
AATGATGCAT GGTCTGAGTT TGAAAACCAG GGCTCGAGGC CCCTCTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006534968.1
CDS	133..3903
Translation	MSLLQSALDFLAGPGSLGGAAGRDQSDFVGQTVELGELRLRVRRVLAEGGFAFVYEAQDLGSGREYALKRLLSNEEEKNRAIIQEVCFLKKLSGHPNIVQFCSAASIGKEESDTGQAEFLLLTELCKGQLVEFLKRVECKGPLSCDSILKIFYQTCRAVQHMHRQKPPIIHRDLKVENLLLSNQGTIKLCDFGSATTISHYPDYSWSAQKRAMVEEEITRNTTPMYRTPEIVDLYSNFPIGEKQDIWALGCILYLLCFRQHPFEDGAKLRIVNGKYSIPVNDTRYTVFHDLIRAMLKVNPEERLSIAEVVRQLQEIAAARNVNPKAPITELLEQNGGYGNSGPSRAQPPCGGTVNSSGVLALAEYDQPYGGFLDILRGGTERLFTNLKDTSSKVIQSVANYAKGDLDISYITSRIAVMSFPAEGVESAIKNNIEDVRMFLDAKHPGHYAVYNLSPRIYRASKFHNRVTECGWAVRRAPHLHSLYTLCRSMHAWLREDHRNVCVVHCMDGRAASAVAVCAFLCFCRLFSTAEAAVYMFSMKRCPPGIWPSHKRYIEYVCDMVAEEPITPHSKPMLVKSVVMTPVPLFSKQRNGCRPFCEVYVGEERVTTTSQEYDRMKEFKIEDGKAVIPLGVTVQGDVLIIIYHARATLGGRLQAKMASMKMFQIQFHTGFVPRNATTVKFAKYDLDACDIQEKYPDLFQVNLEVEVEPRDRPSREAPPWENTSLRGLNPKILFSNREEQQDILSKFEEKEPETGLDNTSPKESQSVLIADGDGSEVSDEEEASFPSEERKPGAGEDTPRLAAGTKQQDLIFDVGMLAAPQEPVQPEEGVDLLGLHSEGDLRPAAPLQACGVPSSNTDLLSCLLEPSDAAQVGPPGDLLGGEAPLLLASPVSPLGLQNNLQGKVPDTVDPFDQFLLSSNSDTQPCSKPDLFGEFLNSDSVASSTAFPSTHSAPPPSCSTAFLHLGDLPAEPSKVIASSSHPDLLGGWDTWADTATPGPASIPVPEGTLFSSAGHPAPPGPNPSQTKSQNLDPFADLSDLSSSLQGLPAGLPAGGFVGAPAPTQKSNSPWQANRPTAPGTSWTPQAKPAPRASEQLRSHFSVIGAREERGVRVPSFAQKPKVSENDFEDLLPNQGFSKSDKKGPKTMAEMRKQELARDTDPLKLKLLDWIEGKERNIRALLSTLHTVLWDGESRWTPVSMADLVTPEQVKKQYRRAVLVVHPDKATGQPYEQYAKMIFMELNDAWSEFENQGSRPLF

Target ORF information:

RefSeq Version	XM_006534905.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus cyclin G associated kinase (Gak), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006534905.2

ORF Insert Sequence:

ATGTCGCTGC TGCAGTCTGC GCTGGACTTC CTGGCGGGCC CTGGTTCTCT TGGCGGAGCT 
GCGGGCCGTG ATCAGAGTGA CTTCGTGGGG CAGACAGTGG AGCTGGGCGA GCTGCGTCTG 
CGGGTGCGGC GGGTCCTGGC CGAGGGAGGG TTTGCTTTTG TTTATGAAGC TCAAGATCTG 
GGAAGTGGCA GAGAGTATGC ATTAAAGCGA TTACTATCCA ATGAAGAGGA AAAGAACAGA 
GCCATCATTC AGGAAGTTTG TTTCTTGAAA AAACTTTCTG GCCACCCCAA TATTGTCCAG 
TTCTGCTCTG CAGCATCCAT AGGAAAAGAG GAGTCGGACA CTGGGCAGGC TGAGTTCCTC 
CTGCTTACGG AGCTTTGTAA AGGACAGCTG GTGGAGTTTC TCAAGAGAGT TGAATGTAAA 
GGTCCTCTGT CCTGCGACAG CATTCTGAAG ATCTTCTACC AGACATGCAG AGCAGTGCAG 
CACATGCACA GGCAGAAACC ACCCATCATC CACAGAGATC TCAAGGTTGA AAACTTACTG 
CTTAGTAACC AGGGGACCAT TAAGCTGTGT GACTTTGGCA GTGCCACAAC CATCTCCCAT 
TATCCTGACT ACAGCTGGAG TGCCCAGAAG CGAGCCATGG TGGAGGAAGA GATCACAAGG 
AACACCACAC CCATGTACAG AACACCAGAA ATTGTAGACC TGTATTCCAA CTTCCCCATT 
GGCGAAAAGC AGGATATCTG GGCACTGGGC TGTATCTTAT ACCTGCTGTG TTTCCGGCAG 
CATCCTTTTG AGGATGGAGC AAAACTTCGG ATAGTCAATG GGAAGTATTC CATTCCTGTG 
AATGACACTC GTTACACAGT CTTCCATGAC CTTATTCGTG CCATGCTAAA GGTCAACCCA 
GAGGAGAGGC TGTCCATTGC TGAAGTGGTC CGACAACTGC AGGAAATTGC AGCAGCTAGG 
AATGTGAACC CCAAAGCTCC CATCACAGAG CTGCTGGAGC AGAATGGTGG CTATGGGAAC 
TCAGGGCCTT CCCGAGCACA ACCACCTTGC GGGGGCACCG TGAACAGCAG TGGAGTTTTG 
GCTCTGGCAG AGTATGACCA GCCCTACGGT GGGTTTCTTG ATATTCTGCG GGGTGGGACC 
GAACGGCTCT TCACCAACCT CAAGGATACT TCCTCCAAGG TCATCCAGTC TGTGGCTAAC 
TATGCAAAGG GTGATCTGGA CATATCTTAC ATCACATCCA GGATTGCAGT GATGTCATTC 
CCAGCAGAAG GTGTGGAGTC TGCAATCAAA AACAATATAG AGGATGTGCG AATGTTTCTG 
GATGCTAAGC ATCCAGGACA TTATGCTGTC TACAACCTTT CTCCAAGGAT ATACCGGGCT 
TCCAAGTTTC ACAACCGGGT CACTGAGTGT GGCTGGGCAG TCAGACGGGC ACCACATCTC 
CACAGTTTGT ATACTCTATG TAGGAGCATG CATGCCTGGC TCCGGGAAGA CCACAGGAAC 
GTCTGTGTTG TGCATTGCAT GGATGGGAGA GCTGCATCTG CTGTGGCAGT CTGTGCATTC 
CTGTGCTTCT GCCGTCTCTT CAGCACTGCA GAGGCTGCTG TGTACATGTT CAGCATGAAG 
CGCTGCCCAC CAGGCATTTG GCCATCCCAC AAAAGGTACA TTGAATATGT ATGTGACATG 
GTGGCAGAGG AGCCCATCAC GCCCCATAGC AAGCCAATGC TGGTGAAATC TGTTGTCATG 
ACCCCCGTGC CACTGTTCAG CAAGCAGAGG AATGGCTGCC GACCGTTCTG TGAGGTCTAC 
GTTGGAGAGG AGCGTGTCAC CACCACGTCG CAGGAATATG ACAGAATGAA GGAATTTAAA 
ATTGAGGATG GCAAGGCTGT CATCCCCCTG GGCGTAACAG TTCAAGGAGA CGTGCTCATC 
ATCATCTACC ATGCCAGGGC CACACTGGGA GGGAGGCTGC AGGCTAAGAT GGCGTCCATG 
AAAATGTTCC AGATCCAGTT CCACACTGGG TTCGTGCCTC GAAACGCAAC CACTGTGAAA 
TTTGCAAAAT ATGACCTGGA TGCTTGTGAT ATTCAAGAGA AGTACCCAGA TCTGTTCCAG 
GTGAACCTGG AAGTGGAGGT AGAGCCTAGA GACAGGCCCA GCCGAGAAGC TCCACCTTGG 
GAGAACACCA GCCTAAGGGG GTTAAACCCC AAGATCCTCT TTTCCAACAG GGAAGAGCAG 
CAGGACATTC TGTCTAAGTT TGAGGAAAAA GAACCAGAGA CTGGGTTAGA CAATACCTCT 
CCTAAGGAGA GTCAGTCTGT CCTGATTGCA GACGGAGATG GAAGTGAAGT ATCAGATGAA 
GAAGAGGCTT CATTCCCCAG TGAGGAGAGG AAACCAGGAG CTGGAGAAGA TACACCAAGG 
CTGGCTGCTG GGACCAAACA GCAAGACTTA ATATTTGATG TGGGCATGCT GGCTGCCCCA 
CAGGAGCCTG TACAGCCCGA AGAAGGTGTT GATCTCCTGG GGCTACACTC TGAGGGGGAC 
TTAAGGCCTG CTGCCCCCTT ACAGGCTTGT GGGGTCCCAT CTAGCAACAC TGACCTGTTG 
AGCTGCCTTC TTGAACCATC TGATGCTGCT CAAGTAGGGC CTCCTGGTGA CCTGCTTGGT 
GGTGAGGCTC CACTGCTGCT AGCAAGCCCA GTTTCTCCTC TTGGGCTGCA GAACAACCTA 
CAAGGAAAAG TCCCTGACAC TGTGGACCCA TTTGACCAGT TCCTGCTGTC ATCCAACTCA 
GACACCCAGC CCTGCTCCAA GCCTGATCTC TTTGGAGAGT TTCTCAACTC TGACTCTGTA 
GCTTCCTCAA CTGCCTTCCC ATCCACCCAC AGTGCCCCAC CCCCATCCTG TAGCACTGCC 
TTCCTGCACC TAGGGGATCT GCCAGCAGAG CCCAGCAAGG TAATAGCTTC ATCCAGCCAC 
CCAGATCTGC TAGGAGGATG GGATACGTGG GCTGACACAG CTACACCTGG GCCAGCCTCC 
ATTCCAGTAC CAGAAGGTAC CCTCTTCTCT TCTGCAGGTC ACCCAGCCCC TCCTGGCCCC 
AACCCCAGCC AAACCAAGTC TCAGAACCTT GACCCATTTG CTGATCTCAG TGATCTCAGC 
TCTAGCCTCC AAGGCCTGCC TGCTGGACTT CCTGCAGGGG GCTTTGTTGG CGCACCAGCC 
CCCACTCAAA AGAGCAACAG CCCCTGGCAG GCAAATCGTC CCACAGCCCC TGGAACCTCA 
TGGACCCCCC AGGCCAAGCC AGCCCCCAGA GCCTCTGAAC AGCTAAGGTC TCACTTCAGT 
GTGATCGGGG CCCGAGAAGA GAGGGGCGTC CGTGTGCCCA GCTTTGCCCA AAAGCCAAAG 
GTCTCAGAAA ATGATTTTGA AGATCTTCTG CCTAATCAAG GCTTCTCTAA GTCTGACAAG 
AAGGGGCCAA AGACCATGGC AGAGATGCGG AAACAGGAAC TTGCCAGAGA TACAGACCCA 
CTCAAATTGA AGCTTTTGGA CTGGATTGAA GGCAAGGAGA GGAACATTCG TGCACTGCTG 
TCCACTCTGC ACACGGTATT GTGGGATGGG GAGAGCCGCT GGACACCTGT GAGTATGGCT 
GACCTGGTGA CTCCAGAGCA GGTGAAGAAG CAGTACCGCC GTGCAGTGCT GGTAGTGCAT 
CCTGATAAGG CCACAGGGCA GCCGTATGAA CAGTATGCCA AGATGATCTT CATGGAGCTG 
AATGATGCAT GGTCTGAGTT TGAAAACCAG GGCTCGAGGC CCCTCTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	Gak
Entrez Gene ID	231580
Full Name	cyclin G associated kinase
Synonyms	D130045N16Rik
General protein information	Preferred Names cyclin G associated kinase Names cyclin-G-associated kinase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5\|5 F

Rattus norvegicus (Norway rat)	Gak	NP_112292.1
Homo sapiens (human)	GAK	NP_005246.2
Bos taurus (cattle)	GAK	NP_001039549.1
Danio rerio (zebrafish)	gak	XP_003198642.2
Pan troglodytes (chimpanzee)	GAK	XP_001140835.1
Mus musculus (house mouse)	Gak	NP_705797.1
Gallus gallus (chicken)	GAK	XP_424873.4
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003715	XP_310244.7
	GAK	XP_001094120.2
Canis lupus familiaris (dog)	GAK	XP_536279.4
Drosophila melanogaster (fruit fly)	aux	NP_001262243.1
Danio rerio (zebrafish)	LOC100151158	XP_001919224.1
Xenopus tropicalis (tropical clawed frog)	gak	NP_001016350.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

Gak cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

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